LOCUS       DASWIY010000001         8193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1847777, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000001 DASWIY010000000
VERSION     DASWIY010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847777"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VGA59_00005"
     rRNA            <1..412
                     /locus_tag="VGA59_00005"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            526..638
                     /gene="rrf"
                     /locus_tag="VGA59_00010"
     rRNA            526..638
                     /gene="rrf"
                     /locus_tag="VGA59_00010"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(699..1622)
                     /locus_tag="VGA59_00015"
     CDS             complement(699..1622)
                     /locus_tag="VGA59_00015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9718087.1"
                     /translation="MTDLAELPPGLTVFERGWLSSNNVLFHGTGPTALVDSGYCTHAG
                     QTLALVRQALGGRSLDLLLNTHLHSDHCGGNAVLQCEYESLETRIPPGVAERVRNWDP
                     VALTYVPTGQQCPRFRIDALLEPGSEVLLGERHWQVHAAPGHDAHSVILFEPRSRNLI
                     SADALWENGFGVIFQELEGEDAFDDVAKTLDVIESLRPLAVIPGHGKVFTGVGQALAN
                     ARRRLDGFVRDPTKHAAHAAKVLLKFKLLELQRLQVTEFARWAAATPYFVLVHRLWFS
                     GVDLTTWIGQLADDLVRSGAAARDGDSIQNL"
     gene            complement(1657..3342)
                     /locus_tag="VGA59_00020"
     CDS             complement(1657..3342)
                     /locus_tag="VGA59_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9718088.1"
                     /translation="MEKYWLKSYSEGVPHEVNPEQYRSLTQLLEESFKKNASRPFSVC
                     MERWMTYGQLDELSAALGAWLQAQGLEPGARVAIMLPNIPQFAVTMAAVLRAGYTCVN
                     VNPLYTARELEHQLKDSGATAIVILENFAKTLEEVMDRTPVKHVVMASMGDLLGFWFG
                     TWITFAVRHLAKMVPPYKLPLSGDRTVTPFNRAIAQGTRLALKPSPQTLDSLAFLQYT
                     GGTTGLSKGAELTHRNIVAAILQAEAWFTPALRRVSDVSKTNSIAALPLYHIFALTLC
                     LLAIRWGSHLTLIPNPRDFGKFVDVLKKRPFHLFPAVNTLFNALLQHPQFKSVDFSQL
                     CLSQAGGMAASEGTAKHWQQVTGCPMVEGWGMSETCAIGTNNVVVSKDFTGTIGLPLP
                     SIDIAIKDDDGQSLALGEPGEICIKGPNVMRGYYRQPEENAKAFTADGYMRTGDIGTM
                     DKRGYIKIVDRKKDMILVSGFNVFPNELENVISLCPGVVECAAIGIPDEKQGEAIKVF
                     VVKNDPTLSEDDVAKYCRHNLTGYKMPKYIEFRDDLPKSNVGKILRRELRTAK"
     gene            complement(3390..4133)
                     /locus_tag="VGA59_00025"
     CDS             complement(3390..4133)
                     /locus_tag="VGA59_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718089.1"
                     /translation="METARKIRNAVLRVELLRQAGEDNPPLRAAVGEIKRVQSRRFAG
                     TYADLLAGGPYAAAAQFFLEELYSDKDYAERDAQFARIAGAIEKLFPEQVAATAVALA
                     GLHAMTEELDQAMALAWLAQVDDEMTAAQRYVAAWREVGRRPDREGQLRVVLGIGHQM
                     ARLTRTAGLRTMLRMMRGPGAAAGLGALQRFLECGFDTFGAMAKRSGGAEGFLEIIEA
                     RESALMSRLFDADLVACETELAHTLGQAP"
     gene            4271..4708
                     /locus_tag="VGA59_00030"
     CDS             4271..4708
                     /locus_tag="VGA59_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718090.1"
                     /translation="MALLQPGLRDGTLSASGRQVFLGVSRALLDGSLPAGAKALQRAL
                     EGLLGRIDGLVAALPPHAQAELSQLLALLASAGGRRALAGVGQPWESAQVAEIQQGLQ
                     SMRVSRITLRQQAYQALHDIVGGAYFSDLGTWSLLGYPGPAKI"
     gene            4719..6338
                     /locus_tag="VGA59_00035"
     CDS             4719..6338
                     /locus_tag="VGA59_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX9718091.1"
                     /translation="MSKLQDPIRDGLKRGWKVMGGPYAEVPPRIVCDVAVIGSGAGAG
                     ITAELLAKAGLKVVIVEEGPLKSSSDFNQHEAEAYPSLYQESAARKTEDKAINILQGR
                     CVGGSTTVNWTSSFRTPVSTLDYWREHFGLDAFANDALAPYFEQAERRLHIEPWLTPP
                     NENNDLLRRGAGKLGIAAAAISRNVKGCWNLGSCGMGCPTNAKQSMLVTTIPEALARG
                     ATLLVETRAERFEFGNGKVRALLCGPVKANGASASTNPADTTIVARHYVLAAGAINSP
                     AVLLRSDAPDPHGRLGLRTFLHPVVMSAGVMPQKVEGWSGAPQTIYTDHFLETQPIDG
                     PIGFKLEAPPIHPVIFASSTVGFGRQQAELLANFPNTHVLLALMRDGFHSDAPGGKVK
                     LRGDGTPVLDYPLSEFVMEGARRAFLAMAEIQFAAGARQVLPVHELARPYASWAEARQ
                     AIQGMAMKPLLAKVVSAHVMGGCGAAATEKQGVVRPDGVHWQLENLSVHDGSIFPTSV
                     GANPQLSIYGIANLLAQSLAKRLSGQDVILA"
     gene            6338..7255
                     /locus_tag="VGA59_00040"
     CDS             6338..7255
                     /locus_tag="VGA59_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9718092.1"
                     /translation="MRSHSTLARLLQAVIFLVLTCSLAWLGWHWSESPLLAVAGAVAI
                     AMIHSVVLAVEFVALRFVSRFDPAPRPTWRELALAWLSESLMALRVFCWRQPFRWREV
                     PDRLEAAPAGPAKRGAVFIHGFVCNRGFWTPWLKELSARGHPFAAVNLQPVFGSIDEY
                     VPIVEDAVQRVTGATGMAPVLICHSMGGLAARAWLRAMSADARVHHIITIGSPHHGTW
                     LARFSRFKNGRQMKMHSDWLGQLERSIEERRNANFTCWYSNCDNIAFPASTGALPGAD
                     NRLVRGAAHVDLGFHPEVMAESLASVSAG"
     gene            7354..8001
                     /locus_tag="VGA59_00045"
     CDS             7354..8001
                     /locus_tag="VGA59_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9718093.1"
                     /translation="MDMLARSEIGRVPAADGDTSLGCSLALLMDELVHGVLVATVGGR
                     LLHANQAAQHELGRGRVLAARRGVLRTCTPEDGKVLQDALAKVAEGKRSLIELTAAGG
                     AVVRLAVLPLKAETGPPNAALLFARESVCDALMLRFFAQSHALTATEEHVLGILCQGF
                     SAPQIAAQMAVAVSTIRSHVRSLCAKTHSSGVRQLVNRVAVLPPVAPPFWVRSVH"
BASE COUNT         1411 a         2604 c         2660 g         1518 t
ORIGIN      
        1 ttggcacctc gatgtcggct catctcatcc tggggctgta gccggtccca agggtatggc
       61 tgttcgccat ttaaagaggt acgtgagctg ggtttaaaac gtcgtgagac agtttggtcc
      121 ctatcttccg tgggcgctgc agatttgagg aagcctgctc ctagtacgag aggaccggag
      181 tggacgcacc tctggtgtat cggttgtcac gccagtggca ttgccgagta gctaagtgcg
      241 gaagagataa ccgctgaaag catctaagcg ggaaactcgt tccaagatga gatctgccgg
      301 ggccttgagc cccctgaaga gtcgttctag accaggacgt tgataggtca ggtgtggaag
      361 cgcagtaatg cgttaagcta actgatacta attgctcgtg cggcttgacc ctataacttt
      421 gatcagtcga tcaaggtgtt atgccaagtg acgcaatcga attgaagctg atttgctcta
      481 tgaattggtt gttttgaccc atggtcaaga cgacgacaag ttatgcctga tgaccatagc
      541 gaggtggtac cactccttcc catcccgaac aggacagtga aacgcctctg cgccgatgat
      601 agtgcggatt cccgtgtgaa agtaggacat cgtcaggctc ttacagcggc gaaaggccca
      661 gcagaaatgc tgggcctttt gttctttgcc gctcagtttc acagattctg aatcgagtcg
      721 ccatccctgg ctgcggcgcc cgagcggacc agatcgtccg cgagctgccc gatccatgtg
      781 gtgaggtcga cgcccgaaaa ccagagtcgg tgcacgagga cgaagtaggg cgtggcagcg
      841 gcccaccgcg cgaactcggt cacctgcaaa cgctgcaatt cgagcagctt gaacttgagc
      901 aggaccttcg cggcatgcgc cgcatgtttg gtgggatccc gcacaaaacc gtccaggcgg
      961 cgtcgcgcgt tggccagcgc ttgcccgacc ccggtgaaaa ctttgccgtg ccccgggatc
     1021 accgccagcg gccgcagcga ctcgatgaca tcgagcgttt tcgccacatc gtcgaaggca
     1081 tcttcgcctt ccagctcctg gaagatgact ccgaacccgt tttcccaaag cgcatcggcc
     1141 gagatcaggt tccgcgaacg gggctcgaac aggatcacgg aatgggcgtc atgcccgggt
     1201 gcggcatgga cctgccagtg ccgttcgccc aacagcacct cgctgccagg ctcgagcaag
     1261 gcgtcgatcc tgaagcgcgg gcactgctgc ccggtgggca cgtaggtcag cgccaccggg
     1321 tcccagttgc gcacgcgctc ggccacgccg ggcggaatgc gcgtttcgag gctctcgtac
     1381 tcgcactgca gcaccgcgtt gccgccgcaa tggtcgctat gcagatgcgt attgagcagc
     1441 agatcgagcg agcggccgcc cagagcctgg cgaaccaatg ccagcgtctg gccggcgtgc
     1501 gtgcaatagc cgctgtcgac cagggcggtg ggaccggtgc cgtggaacag aacgttgttg
     1561 ctcgacagcc atccacgctc gaaaacggtg aggccgggcg gaagttccgc aagatccgtc
     1621 atgcggaaag actaaccgaa aagccgcctc gatctgctat ttggcggtcc gcagctcccg
     1681 gcgcaggatc ttgcccacgt tgctcttggg caggtcgtcc cggaattcga tgtacttggg
     1741 catcttgtag ccggtgaggt tgtgccggca gtacttcgcg acgtcgtcct cggacagtgt
     1801 gggatcgttc ttgacgacga agaccttgat cgcctcaccc tgtttttcgt ccggaatacc
     1861 gattgcggcg cattcgacca cgccggggca cagcgaaatg acgttctcga gctcgttcgg
     1921 gaacacgttg aagccgctca ccaggatcat gtccttcttg cgatcgacaa tcttgatgta
     1981 gccgcgcttg tccatggtgc cgatatcgcc ggttcgcatg tagccgtcgg ccgtgaacgc
     2041 ctttgcgttc tcctccggct gcctgtaata gccgcgcatc acgttggggc ccttgatgca
     2101 gatctccccc ggctcgccca gggccaggga ttgcccatcg tcgtccttga tcgcgatatc
     2161 gatgctgggc agcggcaagc cgatcgtgcc ggtgaagtcc ttgctgacca cgacgttgtt
     2221 ggtgccgatc gcacaggtct cgctcatgcc ccagccctcg accatcgggc agcccgtgac
     2281 ctgctgccag tgtttcgcgg tgccctcgga cgcggccatg ccgccggcct gcgacaggca
     2341 cagctgcgaa aaatcgaccg acttgaactg aggatgctgc agcagcgcgt tgaacagggt
     2401 attgaccgcg gggaagaggt ggaacgggcg cttcttgagc acgtcgacga atttgccgaa
     2461 gtcgcgcgga ttcggaatca aggtcaggtg cgagccccag cgtatggcca gcaggcacaa
     2521 ggtgagcgcg aagatgtggt acaggggcag cgccgcgatg ctattggtct tactcacatc
     2581 cgagacgcgc ctgagcgccg gtgtgaacca ggcctcggcc tgcaggatgg cggccacgat
     2641 gttgcgatgg gtcagctccg cacccttgga caatccggtc gtcccgccgg tgtattgcag
     2701 aaatgccaat gagtcgagcg tctgcgggct cggcttcagc gccagcctgg tgccctgcgc
     2761 tatggcgcgg ttgaacggcg tcaccgtgcg atcgccggaa agcggcagct tgtagggggg
     2821 aaccatcttg gccagatgcc tcaccgcgaa ggtgatccag gtgccgaacc agaaccccag
     2881 caggtcgccc atggaagcca tcacgacgtg cttgaccggg gtgcgatcca tcacttcctc
     2941 gagcgtcttg gcgaagtttt ccaggatgac gatcgccgtc gcgcccgaat ccttgagctg
     3001 gtgttcgagt tcgcgcgccg tgtacagcgg gttgacgttc acgcaggtgt agcccgcgcg
     3061 caggacggcc gccatcgtaa cggcaaactg cgggatgttg ggcaacatga tggcgacgcg
     3121 cgctccgggc tccaggccct gggcctgcag ccaggcgccg agcgccgccg acagttcgtc
     3181 gagctgccca taagtcatcc accgttccat gcacaccgag aacggacgcg aggcgttctt
     3241 cttgaacgat tcctccagca gctgcgtcag cgatcggtat tgctccggat tgacctcgtg
     3301 gggcacgccc tcagagtagc tcttgagcca gtacttttcc atttcaagac tccttgggca
     3361 gcgattgtcg aaggagtcgg cgccgaaagt tagggggctt gtcctaaggt atgggccaat
     3421 tcggtctcgc acgcgacaag atccgcgtcg aacagccgcg acatgagagc cgactcgcgc
     3481 gcctcgatga tttccaggaa cccctcggct ccgccggaac gcttcgccat cgcgccgaag
     3541 gtgtcgaagc cgcattccag gaagcgttgc agcgcgccca atccggctgc tgccccgggt
     3601 ccgcgcatca tcctgagcat cgtgcgcagg cccgcggtgc gggtgagccg tgccatctgg
     3661 tgcccgatgc ccagtacgac gcgcagttgc ccttcgcgat ccggccgccg gcccacctca
     3721 cgccacgcgg cgacatagcg ctgggccgcg gtcatttcgt cgtccacctg cgccaaccag
     3781 gccaaggcca tcgcctgatc cagttcttcg gtcatcgcgt gcaatccggc cagcgcgacc
     3841 gcggtggctg cgacctgctc ggggaacagc ttttcgatcg cgcccgcgat gcgcgcgaat
     3901 tgcgcgtccc gctcggcata gtccttgtcg ctatagagtt cctcgaggaa gaactgcgcc
     3961 gcggccgcgt aaggaccgcc ggccagcaag tcggcgtagg tgccggcgaa acgccgcgac
     4021 tggactcgct tgatttcgcc gacagccgcg cgcagcggcg gattgtcttc cccggcttgc
     4081 ctcagcaact ccacccggag gactgcattg cggatttttc gtgcggtttc catgccaaca
     4141 ccgagtctat ctaggcgcgg gcctctacag atggcgtcgc cgcccatgcc atagtccaaa
     4201 gatgcaaaga aggaccttgc tgacactggg cgcgatttcc gccgcggcac tggcgctggg
     4261 cggtggcaca ttggcgctgc tgcaaccggg gctgcgagac ggcacgctgt cggcctcggg
     4321 acgccaggtt tttttgggcg tgagccgcgc gcttctcgat ggcagcctgc cggccggcgc
     4381 caaggcgctg caacgcgcgc tcgaagggct gcttggcagg atcgacggcc tggttgccgc
     4441 attgccgccg catgcccagg cggagctttc ccaattgctg gcgctgctgg cgagcgccgg
     4501 aggccggcgc gcgctggcgg gagtcggtca accctgggag agcgcgcagg tcgccgagat
     4561 tcaacaaggc ttgcagtcga tgcgggtctc ccgcatcacc ttgcgccagc aggcctacca
     4621 ggccttgcac gatatcgtcg gcggcgccta tttctccgat ctgggaacct ggagcctgtt
     4681 gggctatccc ggtccggcaa aaatttgaag gcgggccgat gagcaaattg caggacccca
     4741 tccgggacgg cttgaagcgc ggctggaagg tcatgggcgg cccctacgcc gaggtgccgc
     4801 ccaggatcgt ttgcgatgtc gccgtcatcg gatccggtgc cggtgccggc atcaccgccg
     4861 aactgctggc gaaagccggc ctgaaggtgg tgatcgtcga ggaaggaccg ctgaagagca
     4921 gcagcgattt caaccagcat gaagccgagg cttacccctc gctgtaccag gaaagtgcgg
     4981 cgcgcaagac cgaagacaag gcgatcaata tcctgcaggg gcggtgcgtg ggcggctcca
     5041 ccacggtcaa ctggaccagc tcgttccgca cgcccgtgtc cacgctcgac tactggcgcg
     5101 aacacttcgg gctcgatgct tttgccaacg atgcgctcgc gccgtacttc gagcaggccg
     5161 agcgccggct ccatatcgaa ccgtggctga caccgccgaa cgagaacaac gacctgttgc
     5221 gccgcggtgc cggcaagctc gggatcgccg cggcggccat ctcgcgcaac gtcaagggct
     5281 gctggaacct cggatcgtgc ggcatggggt gccccaccaa tgccaagcaa tcgatgctgg
     5341 tcaccaccat tcccgaggca ctggcgcgag gcgctacctt gctggtggaa acgcgggccg
     5401 agcgcttcga gttcggcaac ggcaaagtcc gggcgctgct ctgcggcccg gtcaaggcca
     5461 acggcgcctc cgcctccacg aacccggccg acacgaccat cgtggcgcgg cactatgtgc
     5521 tcgcggcagg ggccatcaat tcgccggccg tgctgctgcg atccgacgcg ccggacccgc
     5581 atgggcgctt gggcctgcgc accttcctgc atccggttgt gatgtcggcc ggcgtgatgc
     5641 cgcagaaggt cgaaggctgg tccggggcgc cgcaaacgat ctataccgac cattttctgg
     5701 aaacccagcc catcgacggt ccgatcggct tcaagctgga agcgccgccc attcatccgg
     5761 tgatcttcgc gtccagcacc gtgggctttg gccggcagca ggccgagctg ctcgcgaatt
     5821 tcccgaacac tcatgtcttg ctggccctga tgcgggatgg ctttcacagc gacgcacccg
     5881 gcggcaaggt caagctgcgc ggcgatggaa cgcccgtgct cgactatccg ctaagtgaat
     5941 tcgtgatgga gggcgcccgc agggccttcc tcgccatggc cgaaatccag ttcgcggccg
     6001 gagcccggca agtgctgccg gtgcacgaac tggcgcggcc gtatgcgtca tgggccgagg
     6061 ccaggcaggc gatccagggg atggccatga agccgctgct ggcgaaggtc gtcagcgccc
     6121 atgtcatggg cggctgcggc gctgccgcca ccgaaaagca gggggtggtg agacccgatg
     6181 gcgtgcattg gcagctcgaa aacctgtcgg tccacgacgg ctccattttt cccaccagcg
     6241 tcggcgccaa tccccagctg tcgatctacg ggattgccaa cctgcttgcc cagtcgctcg
     6301 caaaacgcct gagcgggcag gatgtaatac tcgcctgatg cgatcgcatt cgacgctggc
     6361 ccggctgctg caagccgtca tcttcctggt attgacctgt tcgctggcct ggctgggctg
     6421 gcattggagc gagtcgccgt tgctggccgt cgcgggtgcc gtcgcgattg cgatgatcca
     6481 ttccgtcgtc ctggcggtcg agttcgtggc gttgcgcttt gtcagccgct ttgaccctgc
     6541 tccccggccg acgtggcgtg aactcgcgct cgcctggctg agtgaatccc tcatggctct
     6601 gcgcgtgttc tgctggcgcc agcccttccg ctggcgcgag gtgccggacc ggctcgaagc
     6661 ggctcctgca gggccggcga agcgcggcgc ggtgttcatc catggtttcg tttgcaatcg
     6721 cggcttctgg acaccctggc tgaaggagtt gagcgcgcgc gggcaccctt tcgcggcggt
     6781 caaccttcag cccgtgttcg ggtccatcga cgagtacgtg ccgatcgtcg aggacgcggt
     6841 acagcgcgtc accggggcaa ccggcatggc gccggtcctc atctgccaca gcatgggggg
     6901 gctggccgcg cgcgcctggc tgcgcgcgat gtcggcggat gcccgcgtcc accacatcat
     6961 caccattggc tcgccacacc atggcacctg gctggcgcgc ttcagccgct tcaagaacgg
     7021 gcggcaaatg aaaatgcaca gcgactggtt ggggcagctg gagcgcagca tcgaggaaag
     7081 gcgcaacgcc aacttcacct gctggtattc caattgcgac aacatcgcgt ttccggcgtc
     7141 caccggcgcc ttgcccggcg ccgacaaccg gctggttcgg ggtgcggcgc atgtggacct
     7201 cggcttccac cctgaggtca tggccgaatc gctcgcgagt gtctcggccg gatagcggcc
     7261 ggtgatatct cacaggaaat gccggcctta ttaaccgccg gcacgtccgg cagattccta
     7321 tgctccgcta acgaacaagt tggggaaacg catatggaca tgcttgcgcg cagcgaaatc
     7381 ggccgggtcc cggccgcgga cggcgacacc tcgctgggtt gctccttggc cctgctgatg
     7441 gacgagttgg tgcacggcgt gctggtcgcc accgttggcg gccggctgct ccacgccaac
     7501 caggcggcgc agcacgaact ggggcgcggg cgcgtgctgg cggcacggcg cggcgtgctg
     7561 cgcacctgca cgccggagga cggcaaggtc ctgcaggatg cgctggccaa ggttgccgag
     7621 ggcaagcgca gcttgatcga attgaccgcc gccggggggg ccgtggtaag gctggcggtg
     7681 ctgccgctga aggcggagac ggggcctccg aatgcggccc tgctgtttgc ccgcgagtcg
     7741 gtgtgcgatg ccctgatgct gcgcttcttc gcccaaagcc acgcgctcac cgccaccgag
     7801 gagcatgtgc tcggcattct gtgccagggt ttctcggcac cgcagatcgc ggcccagatg
     7861 gcggtggcgg tttcgacgat ccgcagccac gtgcgcagcc tctgcgccaa gacccattcg
     7921 agcggggtgc gccagctcgt caaccgggtc gcggtcctgc ctccggtggc gccgccgttc
     7981 tgggttcgct cggtccattg agtttcgcgg ccgggctgcg attccccgtg tcgcccgtca
     8041 tgctagagtg cgcctggatt gaggggacgc acaccatgac catcccaggg gacgccgaaa
     8101 agctgatcgc tgccttgagc cccgctttgc ggaggctggc cctcaggggc acggtccgca
     8161 gctaccgcaa gaactccgtc atcatcaatg aag
//
LOCUS       DASWIY010000002         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2587168, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000002 DASWIY010000000
VERSION     DASWIY010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2587168"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VGA59_00050"
     CDS             <1..190
                     /locus_tag="VGA59_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9718094.1"
                     /translation="EAPVLAIQGEDDPYGTLRQIESIQPTSGPFAAQILPQCGHSPHR
                     DQPDRTRQCIVEFLASLP"
     gene            complement(192..914)
                     /locus_tag="VGA59_00055"
     CDS             complement(192..914)
                     /locus_tag="VGA59_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEX9718095.1"
                     /translation="MQLQDLLFSQGFGTRRVCAGLIQHGHVTVAGHTVTHADAEFIPL
                     GLRYSVDGVDWEYQEKAYLMLHKPTGTECSRKPSAYPSILTLLPPPLRQRPNKGAVPG
                     VQPVGRLDQDTTGLLLLTDDGQFIHRMSSPRRHVAKVYEVTARHAVEDRQLERLLQGV
                     TLEDDPRPVRAAACERAGDRQFRLTLTQGKYHQVKRMIAAVGNRVEALHRSRIGALVL
                     PADLSPGQWRWLGCADLAALDA"
     gene            1008..1439
                     /locus_tag="VGA59_00060"
     CDS             1008..1439
                     /locus_tag="VGA59_00060"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HEX9718096.1"
                     /translation="MRSRTKYFCLVVSLVLGPPASAEQWFSVSGPGADADGARVEVDL
                     DSVHARGQGGESVIRVTFDALQTHRAGFRFRSIIATAQFDCQRRIISLTSAAYYDGPA
                     GKGLRLGADSSGKQAGMPPALLDSIPATALRALVKATCTTS"
     gene            complement(1480..1662)
                     /locus_tag="VGA59_00065"
     CDS             complement(1480..1662)
                     /locus_tag="VGA59_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718097.1"
                     /translation="MHQSPQKPLARRRDKYPDFDLTDSQAERWLHRDNQPKARKYPSR
                     EESAREARWDKQNLGK"
     gene            complement(1811..2113)
                     /locus_tag="VGA59_00070"
     CDS             complement(1811..2113)
                     /locus_tag="VGA59_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718098.1"
                     /translation="MRKVFPCAALALLAGCSVVPPAAWTFDPARRAPKAALPVQELAV
                     LNDRVAQLQLERNGIRTRIAGESNVWERQRLYAQLHSVGMELSPLERRLAGVAAGR"
     gene            2357..>2910
                     /locus_tag="VGA59_00075"
     CDS             2357..>2910
                     /locus_tag="VGA59_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I9 family protein"
                     /protein_id="HEX9718099.1"
                     /translation="MRYFAVLGYLAFFACSIAAAPLPESSHPVPGPNAARPAHLVDRD
                     GDRVSDGLQATIAAARPGDLFPVIVTHSGPGNAAAARQAVGFFVLHREFRIINGFAAT
                     MTAAQIRALSQRAGVFRIEEDFPVSIKLDAARPDFGIDAVRTSFEVSGAGINGCIVDT
                     GVDPNHEQLNNVVIPFFDAINGRTL"
BASE COUNT          511 a          959 c          916 g          524 t
ORIGIN      
        1 cgaagcgcct gtattggcga tccagggcga ggacgacccc tatggaacgc tgcgccagat
       61 cgagagcatc cagccgacat cgggtccgtt cgccgcgcaa atattgccgc aatgcggaca
      121 ttcgccgcac cgcgaccagc ctgacagaac ccggcaatgc atcgtcgaat tcctggcgag
      181 cctgccttga atcaagcgtc cagcgccgcc aggtcggcgc agcctagcca gcgccattgg
      241 ccgggcgaca ggtccgcggg gagcaccagc gcgccgatcc gcgagcggtg cagcgcctcc
      301 acgcggttgc cgacggcggc gatcatccgc ttgacctggt ggtactttcc ctgcgtgagg
      361 gtcaggcgaa attgccggtc cccggcgcgt tcgcaggcag ccgcgcgcac cggccgcgga
      421 tcgtcctcga gcgtcacacc ctgcagcagg cgttcgagct gccggtcttc gaccgcgtgc
      481 ctggccgtca cttcgtagac cttggccaca tggcggcggg gcgaactcat gcgatggatg
      541 aactggccat cgtccgtcag caatagcagc ccggtcgtgt cctggtccag gcgtcccacc
      601 ggctgcacgc ccgggacggc acccttgttc ggccgctggc gcaagggcgg cggcaataag
      661 gtcaagatgc tgggataggc cgatggcttg cgcgagcact cggtgcccgt aggcttgtgc
      721 agcatcaggt aggctttttc ctggtattcc caatcgacac cgtccacgga atagcgtaaa
      781 cccagcggga tgaattccgc atcggcatga gtgaccgtgt ggcccgcgac cgtgacatgg
      841 ccgtgctgaa tgaggcctgc gcaaaccctt cgtgtcccaa aaccctggga aaacaggaga
      901 tcctgcaact gcatggagcg agtatctcaa gccgggcacg gcaaactgcc tcggcctcat
      961 gtgaaaatcc ggcacgcggg tgccacgcgg ccttccggcc cacctcaatg cgatcgcgaa
     1021 ccaagtactt ctgcctcgtt gtctcgttgg tcctgggccc tccagccagc gccgagcagt
     1081 ggtttagcgt atcgggcccc ggcgccgatg ccgacggcgc ccgggtcgag gtcgacctcg
     1141 actcggtcca cgcgcgcggc caggggggtg aaagcgtcat ccgggtcacc ttcgacgcgc
     1201 tccagacaca ccgcgcgggc tttcgcttcc gttcgatcat cgccaccgcg cagttcgact
     1261 gccagcgacg aatcatcagc ctgaccagcg ccgcgtatta cgacgggccg gccggcaagg
     1321 gcttacgcct gggcgcggac agctcaggca agcaagcggg catgccaccc gcactgctcg
     1381 acagtattcc ggcgacggct ctgagggcat tggtcaaggc tacttgcacg accagttaaa
     1441 cctcagccgc gtgacaagca cggccctgcc gcttcgccgc tatttgccca ggttctgctt
     1501 gtcccagcgc gcctcacgcg ccgactcctc gcgacttggg tatttccggg ccttgggttg
     1561 gttatcgcga tggagccagc gctcggcttg ggaatcggtg aggtcgaaat cgggatattt
     1621 gtcgcgacgc ctcgcaagag gcttttgcgg cgattgatgc atggccgtcc ttgaacgtgc
     1681 ttgggttgaa tgggcaagct gccgtcgccc gagcacaatc tagaacgtcc gtggcccggc
     1741 ggctgtagga cgggagcgca taggtgtagg acaaagcgaa gttgactgtc gagttcgcgc
     1801 cggccgcccg ctagcgcccg gcggcgacac ctgccaaccg gcgttccagc ggtgacaact
     1861 ccatcccaac gctgtgcaat tgcgcgtaaa gacgctggcg ctcccagacg ttgctctcgc
     1921 cggcgatccg cgtgcgaatt ccattgcgtt ccagctggag ctgggccaca cgatcgttga
     1981 gcacggcgag ctcctggacc ggcagcgccg ctttcggcgc ccgccgggcc ggatcgaatg
     2041 tccaggccgc gggcggcacg acggaacagc cggccagcaa ggccagcgcg gcacagggaa
     2101 agacttttcg catggcgacc actcccgaca acagggcttc atgttagccg cagccggcgg
     2161 cgtgcgggct gcatccagcg ccacattcat cggatgaaca tggcctcgtt cgctcctgcc
     2221 aacgcaccag ggtctgccat atacaccatc ggatgtattg agcgaatcac ttgtccggac
     2281 tatgtttcgg cacgtgtcaa ccagcgtgct gactcggctc gcacgcgccc gctgcggcgt
     2341 agggaggaat ccaaaaatgc gctatttcgc cgttctgggt tacctggcgt tttttgcctg
     2401 ctcgatcgcg gctgcgccac tgcctgaatc ctcccacccc gttccgggcc cgaatgccgc
     2461 gcgacccgcg cacctcgtcg accgtgacgg cgatcgggtt tcagacggct tgcaggcgac
     2521 catcgcggca gcgcgacccg gcgacctgtt ccccgtcatc gtcacccaca gcggcccggg
     2581 caatgcagcg gctgcgcgac aggcggtggg tttcttcgtg ctccatcgcg agttcagaat
     2641 tatcaacggc tttgccgcca ccatgacagc agcccagatc agggctttat cgcagcgagc
     2701 aggggtgttc aggatcgagg aagattttcc cgtttcgatc aaactcgacg ccgcccgccc
     2761 cgacttcggc atcgatgcgg tgcgcacgag cttcgaggtg agcggagcgg gcatcaatgg
     2821 gtgcatcgtc gataccggtg tcgacccgaa ccatgagcag ttgaataacg tcgtgattcc
     2881 gttttttgat gcgatcaatg gccggacgct
//
LOCUS       DASWIY010000003         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_369951, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000003 DASWIY010000000
VERSION     DASWIY010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369951"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1256
                     /locus_tag="VGA59_00080"
     CDS             204..1256
                     /locus_tag="VGA59_00080"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEX9718100.1"
                     /translation="MPFAHLPRIPLGRFPTPLEPLPRLSRLLGGPRLWAKRDDLTGPG
                     MGGNKTRKLEFLFGQARAARAQAVATFGGPQSNFARQMAAAASSLGLEAHCFYFVPRP
                     RRLEGNLLLAELAGARLHFIPFGQSAEPSMTLDQACRLVRWVTRLTPTCWGKRLYFMP
                     VGGHTAAGCAGYALAAAEIEDQLRERGVEHATLVTAAGTGGTLAGLMAGFHLRDSPHS
                     VLGIDVGNLWRGFPQAIAELASAVCALGGQPHRFAAADVPLIERAYVGRAYARPTAAG
                     LEALQLAARQEGMFLDPVYTGKAMAGLIDLIRQGRWSARDNVVFLHTGGGPAIFAKAA
                     SQDKNLSLAPRALRLP"
     gene            complement(1253..>1763)
                     /locus_tag="VGA59_00085"
     CDS             complement(1253..>1763)
                     /locus_tag="VGA59_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718101.1"
                     /translation="AWLAVQRDWRGAARLAALAVVPFGIFQLALWGWFGQLGIGSGGD
                     LSTPFEIIPFMGLWRIGAVNPPALALFAAIFGPMVVAPSVWGIAAALRRLWRRDYALA
                     VFVLAANAAIIPFTPFSTFREPLGLTRLATGLVMAVVLYGAHARSRRVLNYSYFWLAA
                     LALVLNDLV"
BASE COUNT          291 a          617 c          589 g          266 t
ORIGIN      
        1 gcacacttgc cccagttgcc accccaggca ctgcccctac ctgccccagt tgccacggcc
       61 gaaggccgcg attcgttgtt gcgctgcata ccatcgccgc attctgcgga ccacggcact
      121 tgcatccgtg gcaagtgtgc aactgccgtg gtggcgggca gtgcaagggt cacacgttac
      181 gttttacgcc ttacgctctc cccatgccct tcgctcacct cccgcgcatc cccctgggcc
      241 gcttccccac gccgctcgaa ccgctgccgc gcctgagccg cctgctgggc gggccgcgcc
      301 tgtgggcgaa gcgcgacgac ctgacgggtc cgggcatggg cggcaacaag acgcgcaagc
      361 tggagtttct gtttgggcag gcgcgcgccg cccgggcgca ggccgtcgcc acgttcggcg
      421 ggccgcagtc caacttcgcc cgccagatgg ccgccgccgc cagctcgctt ggcctggagg
      481 cgcactgctt ctacttcgtg ccccggccgc gccggttgga gggcaacctg ctgctggccg
      541 aactggccgg ggcgcgtctg cacttcatcc cattcggcca gagcgccgag ccctctatga
      601 cgctcgatca ggcctgccga ttggtgcgct gggtgacgag gttgactcca acgtgctggg
      661 gcaagcgcct ctatttcatg ccggtgggtg ggcacacggc ggccggctgc gccgggtatg
      721 cgctggcagc ggccgagatc gaagatcagc tgcgcgagcg gggtgtcgag cacgccacgc
      781 tggtgacggc cgccgggacg ggcggcacgt tggccgggtt gatggccggc tttcacctgc
      841 gggacagccc gcacagcgtg ttaggaattg acgtgggcaa tctttggcga ggcttccccc
      901 aggcgattgc ggaactggcg tcggcggtgt gcgcgctggg cggccagccg caccgttttg
      961 ccgccgccga tgtgccgctc atcgagcgcg cctacgtggg ccgggcctat gcccgcccga
     1021 cagccgccgg cctggaagca ctgcaactgg ccgcgcgcca ggagggaatg ttcctcgacc
     1081 cggtatacac gggcaaggcg atggccggat tgattgacct cattcgccag gggcggtgga
     1141 gcgcgaggga taatgtcgtg ttcctgcaca ccggcggcgg gccggcgatc tttgccaagg
     1201 ccgcgtcaca ggacaagaac ctgagtcttg ccccgcgtgc cttgcgccta ccctaaacca
     1261 gatcattgag cactagagca agcgccgcca gccagaagta agagtagttg agcacgcgcc
     1321 gcgaacgtgc gtgcgcgccg taaagtacca ccgccatcac cagtccggtg gccaaccggg
     1381 tcaggcccag cggctcacga aacgtagaga acggcgtgaa gggaatgatg gcggcgtttg
     1441 cagccagcac aaacactgcc agcgcgtagt cgcgccgcca caatcggcgc agcgcggccg
     1501 caatgcccca cacgctcggc gcgacgacca tcggcccgaa gatggcggcg aacagggcca
     1561 gcgccggcgg gttgaccgcg ccgatccgcc acaggcccat gaaggggata atctcgaacg
     1621 gcgtgctcaa atctcccccc gagccgatgc ccaactggcc gaaccacccc cacagggcaa
     1681 gctggaagat tccgaacggc acgacggcca gagccgccaa tcgggccgcg ccgcgccaat
     1741 cgcgctggac tgccagccag gcc
//
LOCUS       DASWIY010000004        14252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1109949, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000004 DASWIY010000000
VERSION     DASWIY010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 14252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14252
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109949"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2955
                     /locus_tag="VGA59_00090"
     CDS             <1..2955
                     /locus_tag="VGA59_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9718102.1"
                     /translation="DDPRAALRWSLTKLRPVVDDEHVHRLRADREHVSFQPSACEVDT
                     LKVQALLASGVEHAPLAALEQAAGLLQGEFLGGLDLPACYRFHQWCMAEREQHGALRR
                     RVLSTLVERLAQEPDQALPHARAMVAADPLAEGAHATLVRLLAAAGRYPEAEQHYAWA
                     RDLLRREISMADGGPLDQAIRRARRALRSPAAVAPPRATPGDDDRPPPEHEIPLVGRT
                     SERALIERALQAGPGTRRLLLFAGEPGIGKSRLLDHLAQAAGGAGHVVMRGRCFEAEM
                     VRPYGLWIDALRSLDASGMPAGTLAQAAPLLAGGENAAGGSREALFDAALGLVRGIAS
                     AQPLALLLDDLQWIDEGSAALMHYVVRQLDPAAPLVAAGAARSGEVDDNPWARSLLQS
                     LAREGRLWQAEVGPLELADAQMLLHAASAELDASDALRQSGGNPLYLLELSRAARRGG
                     QGTGRSLDTLIEQRLHALDEGARDLLAWAAAMGRELRPEPLATATGLPVAQVLSRLEL
                     FTRRGLLAPTGEGHFDFTHDLVRESVYRALSQPRRRAIHRQIAQALQAASTADPRLHG
                     EIVRHAALAGDAWATARACLAAGDHCLRVFANRDAAAVAERGLAWLEDVPHGTDRVVL
                     EIGLLRVRLLAVAARPGGTTMTALAHRLQRAIASAEALELHAAAAVGWDALSIWQQQA
                     SDIEGAQQATLSAEQATRRADAATHCRQLATSGRCLLDIEADMARGRALLDEAEALAA
                     GLQLKVMELAWGRGLLARAAGELDAAHAALERATAMAAAAENRWREYECMVWSAIIDW
                     ERQRYADVLEQTGSIAEVARRMGETQAPFAEALAALARLRRGDAGAATALAAALQALR
                     DIDDKAHLAYALNEAAALALDDGRDDEAAACAAEALAAAQAVRRPTEVAVASARLAAA
                     RRDAALLPQAPTGPTASARAAAALKDAAQRLTTLLPTPSRQTLAPHPPGDAPCHESSS
                     SAASTSLRPTSS"
     gene            2898..3146
                     /locus_tag="VGA59_00095"
     CDS             2898..3146
                     /locus_tag="VGA59_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX9718103.1"
                     /translation="MSRIVVERSFDIPQTDELMKEVADRERPCLAAYQATWKRSLLSS
                     DRKRMICEYEAADAETMRRIEREAGAQFDRVWVGDVVE"
     gene            complement(3165..4547)
                     /locus_tag="VGA59_00100"
     CDS             complement(3165..4547)
                     /locus_tag="VGA59_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9718104.1"
                     /translation="MTHETAIQTFKAGLRGQVFRQGDAGYDETRALYNGMVDKRPLLI
                     ARCADVADVIAAVQFGREQGLLTAVRGGGHNGPGLGSCDGGLVIDLSAMKGARVDPQE
                     RTVRVGPGCTQGDVDHAAHAFGLAVPAGIVSTTGVAGLTLGGGHGYLSRKYGLTVDNL
                     LEADVVLADGSFVTASASSHPDLYWALRGGGGNFGVVTSFLFRAHPVDQVYGGPMFWE
                     VKDARRIMQWYREFLPRSPRELGAFVGLKTVPSADPFPKEIQGKKICALISCYGGPVG
                     EGEELMKQVRKELPPPILDWMSAMPFTALQSLFDPLLPKGMQWYWKGDFVKELPDAAI
                     DVHLKYAAEAPSELSLMHLYPIDGAVHEVAPHETAWNCRDATWSMVIAGIDPQPSKAA
                     AVSAWARSYWEAVHPYTMGGAYVNFMMEEGEDRIRATYGENYERLAAVKHRYDPGNFF
                     RVNQNIRPAA"
     gene            complement(4658..5041)
                     /locus_tag="VGA59_00105"
     CDS             complement(4658..5041)
                     /locus_tag="VGA59_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718105.1"
                     /translation="MNRHFASFLTTTTTAAAVLAVAAIASGNAYADDITIDTTPFVST
                     KTRAEVRAEVMGQSMTSASSEWATQLNHVPQLNSGYTREQARAEYVAAREEVMARNAE
                     DSGSSYFAALPRGRDSAVITAGPAR"
     gene            complement(5284..5883)
                     /locus_tag="VGA59_00110"
     CDS             complement(5284..5883)
                     /locus_tag="VGA59_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9718106.1"
                     /translation="MASAYHEGMRRLQDRFDTRRLADRLAELRSRTSFTEDDRGFIQT
                     RRLFFFATADGEGHPDCSFKAGDPGFVRVTADNELAFPSYDGNGMFRSLGNVLVNPAV
                     ALLFIDFESPRRLRINGRASFDEGDPLLGQFTGAQLVVRVRAERIFPNCPRYIPRMAL
                     VEPSAYVPREGHTPPVPQWKNVPAFREVLPAGDPARDAS"
     gene            complement(5887..6216)
                     /locus_tag="VGA59_00115"
     CDS             complement(5887..6216)
                     /locus_tag="VGA59_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718107.1"
                     /translation="MDAKQLPLDGRRFDGIVLERGKTAGDADTLLFTGGRFHSSACDP
                     YGYGDGPYTANPSGDGIAFEAQTQNANGSRLLWHGVVRGPRLDATLTILRGGSTVGEK
                     WVLAGEC"
     gene            complement(6356..7660)
                     /gene="aceA"
                     /locus_tag="VGA59_00120"
     CDS             complement(6356..7660)
                     /gene="aceA"
                     /locus_tag="VGA59_00120"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX9718108.1"
                     /translation="MTREQQIAQLEKDWAENPRWKGIKRGYSAADVVKLRGSLQVEHT
                     LAKRGAEKLWNLINTEPFVATLGALTGNQAMQQVKAGLKAIYLSGWQVAGDANSNGEM
                     YPDQSLYSVDSVPKVVKKINATFTRADQIQWSEGKSAGDEDFVDYFAPIVADAEAGFG
                     GVLNAFELMKSMIEAGAAGVHFEDQLAAAKKCGHMGGKVLVPTREAVSKLVAARLAAD
                     VMGTPTILLARTDAEAADLVTTDVDENDKPFFTGERTVEGFFRSRKGVEQAISRGLAY
                     SEYADLIWCETGTPDLAFAKAFADAIHAKFPGKLLAYNCSPSFNWKKHLDDATIARFQ
                     KELGAMGYKFQFITLAGFHSLNYSMFNLAYGYARSNMTAFVEMQQAEFAAADKGFTAV
                     KHQREVGTGYFDAVATTIEAQASTAALKGSTEDEQFFEKKAA"
     gene            8129..9055
                     /locus_tag="VGA59_00125"
     CDS             8129..9055
                     /locus_tag="VGA59_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9718109.1"
                     /translation="MSPRADRLFAYACLALSMSLVGSYVALSKPLVAAIPVFLLAWLR
                     FGIGGLAMLHWLRKPSQEAPMTRQTKMLVFLESFLGNFLFSILMLFGVSLTTAVSAGV
                     IMAAIPAVVALLSWTFLRERLAPRIWAAIACAVLGIALLGLAQPEARTAGSATTIAGA
                     SAAWLGNLLVFGAVLCEASYAVIGKKLTGVLGPKRITSLINVWGFVLVTPFGLYTALR
                     FDFTGVQPGIWTLLVFYSLAASIWTVWLWMTGLKTVPAAQGGVFTVMLPVSAALVGVF
                     ALGERLGALQLLAFVLALAGVVLATVPVKARM"
     gene            complement(9039..9560)
                     /gene="rraA"
                     /locus_tag="VGA59_00130"
     CDS             complement(9039..9560)
                     /gene="rraA"
                     /locus_tag="VGA59_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HEX9718110.1"
                     /translation="MTIASFATCDLCDAHEEDQSGAFRVLPPVFRDFGAAGAFAGRVA
                     TVRCIEDNSRVREAVNSPGEGRVLVVDGGASVRRALLGGNLALAAARNGWAGIVVDGA
                     VRDAAELAACGIGVKALALMPMRSVKRGEGQRDLPVVVQGIAVRPGDWLYADADGIVV
                     SSEPAAQAPTSLL"
     gene            complement(9557..9718)
                     /locus_tag="VGA59_00135"
                     /pseudo
     CDS             complement(9557..9718)
                     /locus_tag="VGA59_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
     gene            9786..10091
                     /locus_tag="VGA59_00140"
     CDS             9786..10091
                     /locus_tag="VGA59_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9718111.1"
                     /translation="MLISLALGERPAWPPQPLAAAAWLYLVVFGSLIALGACLYHLAA
                     ASPAVATSYAFVNLLIAPYLGVFVAAETVTAGEWIACGVLLAGVFLILRGKTPQKPA"
     gene            10224..10658
                     /locus_tag="VGA59_00145"
     CDS             10224..10658
                     /locus_tag="VGA59_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEX9718112.1"
                     /translation="MATAKKTAAKKAPAKKAAAKKAPAKKAAAKKAPAKKAPAKKAVA
                     KKAAPAKKAAAKKAPAKKRTPNAAFMKALTPSPQLAAVVGPAPLPRTEVVSKLWAYIK
                     KNKLQDSVNKRMVNADAKLKEIFGKAQVSMFEMAGLIGKHVK"
     gene            complement(10905..12629)
                     /locus_tag="VGA59_00150"
     CDS             complement(10905..12629)
                     /locus_tag="VGA59_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9718113.1"
                     /translation="MSTAPAQIAGHLLVECLLAQGVTHVFGVPGESYLAVLDGFHAYR
                     ERIRFIVNRQEGGAAFMAEAHGKLTGRPGVCFVTRGPGATNASIGVHTAYQDSTPMVL
                     FVGDVASDQRDREAFQEVEYTSFFGPSTKGMAKRVERIDEPDRIPEYVARAFATAMNG
                     RPGPVVLVLPEDMLVRRTAVRPLPRVEPVQAWSDPGSLREVRELLLQATKPFVIAGGG
                     GWTVQAAQALQRFAENWKLPVGNAFRFQDTFDNHHPLYAGDVGIGVNPRLAARVKESD
                     LVLAVGPRLGEMTTGGYTLLQAPKARQKLVHIHAGAEELNRVYQADVAINATMNAAAR
                     SLEALAAPPEVPWSAWTEAAHADYEANLVPQPLAGLQGDTAAGVVNMPAIVAVISKHL
                     PADAVLTNGAGNFASWMHRYFKHHGLAKGFKTQLAPTVGAMGYGVPAGIAANLVSGRT
                     VLTMAGDGDFLMNGQELATAVQHGARTIVVLLNNGMYGTIRMHQERDYPRHVSGSDLA
                     NPDFAALARAYGYVGVRITRTAEFEPELVAALEREEGTLIEVMLEPEAITTRASLSAI
                     TAAALKNK"
     gene            12670..13320
                     /locus_tag="VGA59_00155"
     CDS             12670..13320
                     /locus_tag="VGA59_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9718114.1"
                     /translation="MTRDIVPATEERQRDADRSQAIILAAARDEFAEHGLGGARMDRI
                     AERAGLNKRLIYYYFEDKETLFQAVLEQAYRDIREQERKLRLLDLKPADAVRRLVEFT
                     WNYYLSHPEFLTLLNSANLHKARHLQASKRAREMNSPLIEMLAEILERGRKDGSFRGG
                     VDPLQLYVSIAGLAYFYLSNSHTLSAIFGRDLMSSKARNERLTHMCDVILGYVLRS"
     gene            13423..>14252
                     /locus_tag="VGA59_00160"
     CDS             13423..>14252
                     /locus_tag="VGA59_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9718115.1"
                     /translation="MPTLRLGIIMHGVTGRMGMNQHLIRSIVAIRAQGGVTLSSGDLC
                     VPDPILIGRNAEKIAALAKAHGIERWGTDLDRALANKDDTVFFDAGTTQMRPSLLAKA
                     IHAGKHVYCEKPIATNLNEAVEIARLAQASGLKHGAVQDKLFLPGLRKLDMLRRAGFF
                     GRMLAVRLEFGYWVFEGDLQPIQRPSWNYRKEDGGGMILDMMCHWRYVLDNLFGEVKS
                     VSCIGATHIPQRWDESGKAYVATADDAAFATAELVGHNGEPVVAQMNMSWATRVRRDD
                     L"
BASE COUNT         2299 a         4759 c         4766 g         2428 t
ORIGIN      
        1 gacgacccgc gcgccgcact gcgatggagc ctcacgaagc tgcgtcccgt cgtggacgac
       61 gagcacgtcc accggctgcg ggccgaccgc gaacacgttt cgttccaacc cagcgcttgc
      121 gaggtcgaca cgctgaaggt ccaggcgctg ttggcgagtg gggtggaaca cgcgccgctg
      181 gccgccctcg aacaggccgc cgggctgctg caaggcgaat ttctcggcgg cctcgacctg
      241 ccggcgtgct accgattcca ccaatggtgc atggccgagc gcgagcagca cggggccctg
      301 cgccggcgcg tcctgagcac gctggtggaa cggctcgcgc aggagcccga tcaggccctt
      361 ccgcacgccc gcgccatggt cgcggcggac ccgctcgcgg aaggggcgca cgccacgctc
      421 gtgcggctgc tggcggccgc cggccgctat cccgaggccg agcagcacta tgcctgggcg
      481 cgcgacctgc tgcggcgcga aatctccatg gccgatggcg ggccgctgga ccaggcgatc
      541 cgccgcgcgc gccgcgcatt gcgctcaccc gccgcggtgg cgcccccgcg agccacgccg
      601 ggggacgacg atcggccgcc gcccgagcac gagattcctc tcgtcggccg gacgagcgag
      661 cgggcgctga tcgagcgtgc gttgcaggcg ggaccgggga cgcggcgcct gctgctgttc
      721 gccggcgagc cgggcatcgg caagagccgg ctgctcgacc acctggcaca agcggccggc
      781 ggcgccggtc atgtcgtcat gcgcggtcgg tgtttcgagg cggagatggt caggccctac
      841 gggttgtgga ttgacgcgtt gcgcagcctc gatgcgagcg gcatgccggc cggcacgctg
      901 gcccaggccg caccgctgct ggccgggggc gagaacgctg caggcggcag ccgcgaagcg
      961 cttttcgatg ccgcgctcgg gttggtgcgc ggcatcgctt cggcgcagcc gctggcgctg
     1021 ctgcttgacg acctgcaatg gatcgatgaa ggttcggcgg cgctaatgca ttacgttgtg
     1081 cgccagctcg atcccgccgc gccgctggtc gccgccggcg cggcgcgctc tggcgaggta
     1141 gacgacaacc cgtgggccag atccttgctg cagtcgctgg cgagggaagg ccggttgtgg
     1201 caggccgagg tcggcccttt ggagctcgcg gacgcgcaga tgctgctgca cgctgcctcg
     1261 gccgagctgg acgcgtccga tgcgctgcga cagtccggcg gcaaccccct gtacctgctc
     1321 gaactgtcgc gtgcggcccg gcgcggaggc caggggaccg gccgcagcct cgacacattg
     1381 atcgagcaac ggttgcacgc actcgacgag ggcgcgcgtg acctgctggc atgggcggcc
     1441 gccatgggac gggaactgcg gcccgagccg ctcgcgaccg caacagggct gcccgtggcg
     1501 caagtgctgt cgcgcctgga actcttcacg cgccgcggac tgctcgcgcc caccggcgaa
     1561 ggccatttcg acttcacaca cgacctggtg cgggagagcg tttaccgggc tttgtcgcag
     1621 ccgcggcggc gcgcgatcca ccgccagatc gcgcaggcct tgcaggccgc gagcaccgcc
     1681 gacccgcggt tgcacggcga gatcgtacgg cacgccgcgc tcgcaggcga cgcatgggcc
     1741 acggcgcgcg cctgcctggc cgccggcgac cactgcctgc gggtgttcgc caaccgcgat
     1801 gcagctgccg tcgccgagcg cgggctggcc tggctggagg acgtgccgca cgggactgac
     1861 cgcgtcgtgc tggaaatcgg gctgttgcgc gtgcgcctgc tggccgtcgc ggccaggccg
     1921 ggcggcacca ccatgaccgc actggctcac cgcctgcagc gtgcgatcgc tagcgccgaa
     1981 gcgctggaac tgcacgcggc ggcagcggtc ggctgggatg cgctttctat ttggcaacag
     2041 caggccagcg acatcgaggg ggcgcaacaa gcgacgctgt cggcggagca ggcgacccgc
     2101 cgggccgatg cggcgacgca ctgccggcag ctggccacca gcggccgctg cctgctggac
     2161 atcgaggccg acatggcgcg ggggcgggcc ctgctggacg aagccgaggc gctggcggcc
     2221 gggctgcagc tgaaagtcat ggagctggcg tgggggcgcg gcctgctggc gcgcgccgcc
     2281 ggcgaactcg atgccgcgca cgcggcgctc gaacgcgcca ccgcgatggc cgccgcggcc
     2341 gagaaccgtt ggcgcgagta cgaatgcatg gtctggtccg cgatcatcga ctgggagcgg
     2401 cagcgctacg ccgatgtgct cgagcaaact gggagcattg ccgaggttgc gcgccgcatg
     2461 ggcgaaaccc aggcgccgtt cgccgaagcc ctggcggcgc tcgcccggct gcggcgcggc
     2521 gatgccgggg cggccacggc actggcggcc gcgctgcagg ccctgcgcga catcgacgac
     2581 aaggcgcacc tggcctatgc cctcaacgag gcggcggcgc tggcgctgga cgacggccgc
     2641 gacgacgaag cggcggcctg cgcggccgag gcactggctg ctgcgcaggc ggtgcggcgg
     2701 cccaccgaag tggccgtggc gagcgcccgg ctcgccgccg cccgccgcga cgcggcactt
     2761 ctgcctcaag cgccgaccgg gccgaccgca agcgcacgcg ccgcagcggc cctcaaggat
     2821 gcggcgcagc ggcttacaac gttgcttcca acgccatccc ggcagactct tgctcctcac
     2881 ccacctggag acgcaccatg tcacgaatcg tcgtcgagcg cagcttcgac atccctcaga
     2941 ccgacgagct catgaaagag gtcgccgacc gcgagcggcc ctgcctcgcc gcctaccagg
     3001 cgacgtggaa gcggtcgctg ctgtccagcg accgcaagcg catgatctgc gagtacgagg
     3061 ccgcggacgc agaaaccatg cgccgcatcg agcgcgaggc cggcgcgcag ttcgaccggg
     3121 tgtgggtcgg cgacgtggtg gaatgaccac gcgcgccgcg ccgtctacgc tgcgggccgg
     3181 atgttctggt tgacgcggaa gaaattgccc gggtcgtacc tgtgcttcac ggccgccagg
     3241 cgctcgtagt tctcgccgta tgtcgcgcgg atgcgatcct cgccttcctc catcatgaag
     3301 ttgacgtagg cgccgcccat cgtgtacggg tgcaccgcct cccagtaact cctggcccac
     3361 gccgaaaccg cggcggcctt cgagggctgc gggtcgatgc ccgcgatcac catcgaccag
     3421 gtggcatcac ggcagttcca ggcggtttca tgcggggcta cttcgtgcac cgcgccatcg
     3481 atcgggtaca ggtgcatgag ggacagctcg ctcggcgcct cggcggcgta cttcaggtgc
     3541 acgtcgatgg ccgcgtccgg cagttccttg acgaagtccc ccttccagta ccactgcatc
     3601 cccttgggca gcagcggatc gaacaggctc tgcagcgccg tgaagggcat cgcgctcatc
     3661 cagtcgagaa tgggcggcgg cagctccttg cgcacttgct tcatcagctc ctcgccctcg
     3721 cccaccgggc cgccgtagca ggagatgagg gcgcagatct tcttcccctg gatttccttg
     3781 gggaacgggt ccgccgaagg caccgtcttc aggccaacga aggcgccgag ctcccgcggc
     3841 gagcgcggca ggaactcgcg gtaccactgc atgatgcggc gcgcgtcctt gacctcccag
     3901 aacatcggac cgccatagac ctggtccacc ggatgcgcac ggaagaggaa gctggtcacg
     3961 acgccgaaat tgccgccgcc gccgcgcaag gcccagtaca ggtcggggtg ggaggaagcg
     4021 ctcgccgtga cgaaactgcc gtcggccagc acgacatcgg cctccagcag gttgtcgacc
     4081 gtcaggccgt acttgcgcga gaggtaaccg tggcccccgc ccagggtcag ccccgccacg
     4141 ccggtggtgg aaacgatccc ggccggcacg gccaggccga acgcgtgtgc ggcatggtcc
     4201 acatcgccct gggtgcagcc gggcccgacc cgcacggtcc gttcctgcgg atcgacacgc
     4261 gcgcccttca tggcactcag gtcgatcacc agcccgccat cgcaacttcc gaggcccggg
     4321 ccgttgtggc cgccgccgcg aaccgccgtc aacaggccct gttcccggcc gaattgcacg
     4381 gccgcgatga cgtcggccac gtcggcgcag cgcgcgatca gcagtgggcg cttgtcgacc
     4441 atcccgttgt aaagggcacg cgtttcgtca tagcccgcgt cgccctgccg gaagacctgg
     4501 ccgcgcaggc ccgccttgaa cgtctggatc gcggtttcat gggtcatgat gagctccttt
     4561 cgtcacagaa gatggttgag ttgcagctcg cttcagggtg gcgtgccttg cgccgcgatc
     4621 gcctgcggcg cggcacgccg gggaaagtgg atcggcttca gcgcgccggg ccggccgtga
     4681 tgacggcgga gtccctgccg cgcggcagcg ctgcgaaata ggacgagccg ctgtcttccg
     4741 cgttccgcgc catcacttcc tcccgggcgg cgacgtattc ggccctcgcc tgctcccgcg
     4801 tatacccgct gttgagctgg gggacgtgat tcaactgcgt cgcccattcg ctggaggcac
     4861 tcgtcatcga ttggcccatg acttcggcac gcacttcggc gcgggttttg gtgctcacga
     4921 acggtgtggt gtcgatggtg atgtcgtccg cgtaggcgtt gcccgaggca atcgccgcga
     4981 cggcgaggac ggcggcggcc gtggtggtgg tggtgaggaa ggatgcgaag tggcggttca
     5041 tggtgatctc cttgagcggg gttgaaatct gggaccggcg gctcgctcaa tgcccgtctg
     5101 tccaggttcg aggccccaag catccggtgt gcgcgtatcg ctttcgcgtc acctgcccat
     5161 gttccgtgtt gccgcttggt atgcgcggac taagccttgt aatcggcgcg tatccagcgg
     5221 cgatccgcgt cccgcatccg cgacgcgccc caggccaagc gactttttat ccattgcccc
     5281 gcttcaggac gcgtcgcggg ccgggtcgcc ggcaggcagc acctcgcgga aggccggaac
     5341 gtttttccac tgcggtaccg gcggcgtgtg gccctcgcgg ggcacatagg ctgatggctc
     5401 gacgagcgcc atgcgcggga tgtagcgcgg gcagttcggg aagatgcgct cggcgcgcac
     5461 gcgcaccacc agctgcgcac cggtgaactg accgagcagc gggtcgccct cgtcgaagga
     5521 tgcccggccg ttgatacgca gccgtctcgg gctttcgaaa tcgatgaaga gaagtgccac
     5581 cgccggattc accagcacgt tgcccagact gcggaacata ccgttgccgt cgtagctcgg
     5641 gaatgccagc tcgttgtcgg ccgtgacccg cacgaacccg ggatcgccgg ccttgaacga
     5701 gcagtccgga tgtccctcac catcggcggt ggcgaagaag aacagccgcc gggtttgaat
     5761 gaagccgcgg tcgtcctcgg tgaaggaagt gcgcgagcgc agttcagcca gcctgtccgc
     5821 cagccgccgc gtgtcgaagc ggtcttgcag gcgccgcatg ccttcgtgat aagcggatgc
     5881 catggctcag cactcccccg cgagcaccca tttttcgccg acggtgctgc cgccccgcag
     5941 gatggtgagc gttgcgtcca ggcgcgggcc gcgcacaacg ccgtgccaga gcagccggct
     6001 gccgttggca ttctgcgtct gtgcttcgaa ggcgatgccg tctcccgatg ggttcgcggt
     6061 gtacggaccg tcgccgtagc catacgggtc gcacgcgctc gaatggaagc ggccgccggt
     6121 gaacagcagg gtgtcggcgt cgccggcggt ctttccacgc tcgagcacaa taccgtcgaa
     6181 gcgccggccg tcgagtggca gttgcttggc atccatggca ggtctccttg tgaaccttgg
     6241 gagagacgaa gcctgacccc cctgcgttgg agcctgcgtt ggaatacgcg cgcaaccggc
     6301 gaaaaaaagc ccggtggccg aaaaccaccg ggctattggc tgatgagcct gtgtatcagg
     6361 cggccttctt ctcgaagaac tgctcgtcct ctgtggaacc cttgagcgcc gcggtcgatg
     6421 cctgggcttc gatggtcgtg gccaccgcat cgaagtaacc ggtgcccacc tcccgctggt
     6481 gcttcaccgc cgtgaagccc ttgtcggcgg ctgcgaactc ggcctgctgc atctcgacga
     6541 aagccgtcat gttgctgcgg gcatagccgt aggccaggtt gaacatcgag tagttcaggc
     6601 tgtggaaacc cgccagcgtg atgaactgga acttgtagcc catcgcgccc agttccttct
     6661 ggaatctggc gatggtcgcg tcgtccaggt gcttcttcca gttgaacgac ggcgagcagt
     6721 tgtaggcgag cagcttgccg gggaacttgg catggatcgc atcggcgaac gccttcgcga
     6781 acgccaggtc gggcgtgccc gtttcgcacc agatcaggtc ggcgtactcg gaatacgcca
     6841 ggccgcggct gatggcctgc tccacaccct tgcggctgcg aaagaaaccc tccaccgtgc
     6901 gctcgccggt gaagaaaggc ttgtcgttct cgtccacatc ggtggtgacc agatcggccg
     6961 cttcggcatc ggtgcgggcc agcaggatgg tgggcgtgcc catcacgtcg gcggcaagcc
     7021 gggcggcgac cagcttggac acggcttcac gcgtgggaac cagcaccttg ccgcccatat
     7081 ggccgcattt cttcgcggcg gccagctggt cttcgaagtg cacgccggcg gcgcccgctt
     7141 cgatcatcga cttcatcagt tcgaatgcgt tgagcacgcc gccgaagccg gcttccgcgt
     7201 cggccacgat cggcgcgaag tagtccacga aatcctcatc gcccgcgctc ttgccttcgc
     7261 tccactggat ctggtcggcg cgcgtgaaag tcgcgttgat cttcttgacc accttcggca
     7321 ccgagtccac cgagtacagc gactggtcgg ggtacatttc gccgttgctg ttggcgtcgc
     7381 cggccacttg ccagccggag aggtagatcg ccttcaggcc ggccttgacc tgctgcatgg
     7441 cctggttgcc ggtgagagcg cccagggtgg ccacgaacgg ctcggtattg atcaggttcc
     7501 acagcttttc ggcaccgcgc ttggccaggg tgtgctcaac ctggagcgag ccgcgcagct
     7561 tgaccacgtc ggcggcgctg tagccgcgct tgatgccttt ccaccgcgga ttttccgccc
     7621 agtctttttc gagttgggca atctgttgtt cgcgagtcag atgggtccag gatgtcatgg
     7681 ctttctctcc aaagttgatg tcgaaactgt tcggctgccg tgatccgaag tgcgccgccg
     7741 atgaatcaac tttagtcttg tacaagagtc ctgcagtctc ttatgtctta tacaagatag
     7801 aatattttct cctttaattt caattagtta tcttaatgat ttctcaatgt ggaacacaat
     7861 atcttgtatt gagaaatttt ggcgcactgc agcacgccct cataccacaa tgcggaacga
     7921 tatttacgcg gagtgaaaaa gagtccccgc cgccaggacc gagcacccgc ctctctcgcg
     7981 cggtccgggt ttgacctcgc tcacgcccag gtagcgctgt gcgggctcca gccggttgat
     8041 tgtcatggcg tgccgcccgg cgcagcgtgc cgcacacgac ggggcgtcgg gcatgatccg
     8101 gtgtcttcga agtaagagcc attctgcaat gagcccgcgc gccgatcgcc tgttcgccta
     8161 cgcctgcctc gcgctctcca tgtcgctggt gggcagctat gtcgccttgt ccaagccctt
     8221 ggtcgccgcc atcccggttt tcctgctggc gtggctgcga ttcggcatcg gcggcctggc
     8281 catgctgcat tggctgcgca aaccgtccca ggaagcgccg atgacgcggc agacgaagat
     8341 gctggtgttc ctcgagtcgt ttctggggaa tttcctgttc tccatcctca tgctgttcgg
     8401 cgtcagcctc accaccgcgg tgtcggcggg tgtaatcatg gccgccattc cggccgtggt
     8461 tgcgttgctg agctggacct tcctgcgcga gcggctagct ccacgaatct gggcggctat
     8521 cgcctgcgct gtcctgggca tcgcgctgct gggtctggcc cagcccgagg cgcgaacggc
     8581 cggcagcgcg accaccattg ccggcgcttc cgcggcctgg ctgggcaacc tgctggtatt
     8641 cggcgccgtg ctctgcgagg cctcctatgc cgtcatcggg aaaaagctca ccggtgtcct
     8701 gggccccaag cgcatcacgt ccctcatcaa tgtctggggc ttcgtgctgg tgactccttt
     8761 cggcctgtac acggctctgc gattcgactt caccggcgtg cagcccggca tctggacctt
     8821 gctcgtgttc tattcgctgg cagccagcat ctggaccgtc tggctctgga tgacgggcct
     8881 caagaccgtg cccgcggcgc aaggcggagt cttcaccgtg atgcttcccg tgtcggccgc
     8941 gctggtgggc gtgttcgcgc tgggcgaacg cctgggcgcg ctgcaactgc tggctttcgt
     9001 gctcgccctg gcaggggtgg tcctcgccac ggtgccggtc aaagcaagga tgtaggagcc
     9061 tgcgcggcag gctccgagct gaccacgatg ccgtccgcgt cggcatagag ccaatcgccc
     9121 ggccgcaccg cgataccctg gaccaccacg ggcaggtcgc gctggccttc gccgcgcttc
     9181 acggaccgca tcggcatcaa cgccagcgcc ttgacaccga ttccgcaggc ggcgagttcg
     9241 gccgcatcgc gcaccgcgcc gtcgaccacg atgcccgccc agccattccg ggccgcggcc
     9301 aaggcaagat tgccgcccaa cagcgcccgg cgcacggaag cgccgccgtc caccaccagc
     9361 acccggccct cgccggggga gttcacggcc tcgcggacgc gcgagttgtc ctcgatgcag
     9421 cggacggtgg ccacccggcc ggcgaaagcg ccggccgcgc cgaagtcgcg gaacacgggc
     9481 ggcaggaccc ggaatgcgcc cgactggtct tcttcatggg catcgcacag gtcgcaggtg
     9541 gcaaacgaag caatggtcat tttccggtga ctttcctgat cggtggcttg gtgttctccc
     9601 tgcgcgccgg cagcaccatc gcgttgtcgc gaggggcgtt gagcatgatg gaggtggagg
     9661 tttccgcaag gatacagaac ttcgtcacga ccgcatccag gtgttccacg tccacgaccc
     9721 ggtttccact ggcgccgggc ccggccgggt tcgccagcga aatgttgtgc ggtggcgcgg
     9781 tgttgatgct gatttctctt gcgttgggcg aacggcccgc ctggccgccg cagcctctgg
     9841 cagccgcggc ctggctctac ctggtggtgt tcggctcgct gatcgccttg ggcgcctgcc
     9901 tctaccacct ggccgcggca tcgcccgccg tggcgaccag ctatgccttc gtgaacctcc
     9961 tcattgcgcc ttatttgggc gtcttcgtgg ccgccgagac cgtcaccgcg ggcgaatgga
    10021 tcgcctgcgg cgtcctgctt gcgggcgttt ttctgatcct cagagggaaa acgccgcaaa
    10081 aacccgcgtg ataaaaacga cacgacagag cgaaaaaagc catttttcgc ttggaaacgc
    10141 gtttcttctc cagtagcatc cgcaatgcca gtgggaatgc agattcgcat tggtgattaa
    10201 tcaacttcca aaaggaaata atcatggcaa ctgcgaaaaa gactgcggcg aagaaagcac
    10261 cggcaaagaa ggccgccgcg aagaaggcgc cggcgaagaa ggccgccgcg aagaaggcgc
    10321 cggccaagaa ggctccggcc aagaaggctg tcgcgaagaa ggccgcaccg gcgaagaaag
    10381 ctgccgcgaa gaaggcgccg gccaagaagc gcaccccgaa cgccgcgttc atgaaggccc
    10441 tgacacccag cccgcaactg gcggctgtgg tcggtccggc gccgctgccg cgcaccgagg
    10501 tcgtgagcaa gctgtgggcc tacatcaaga agaacaagct gcaggactcc gtcaacaagc
    10561 gcatggtcaa tgccgacgcc aagctgaagg aaatcttcgg caaggcccag gtctccatgt
    10621 tcgagatggc cggcctgatt ggcaagcacg tcaaataaag cttcggagcg cggggaaacc
    10681 tccggagcgc ggggtgaggg gcccacgcca tgcgtgggga tcgaccagcg caggaggttc
    10741 tcgaaggagc cggctcgatg ccggcgactg agaccgactc ccgcaaacca gggccgccca
    10801 agcggccctt ttcttttggg gtctgtcatg ccggacaccg gatcgggccc ggcgcaggcc
    10861 tgatccggca tccatgaatc gcggagccca ggccgcaagc acgattattt gttcttcagc
    10921 gcggccgccg tgatcgcgct caggctggcc cgggtcgtga tcgcttcggg ctcgagcatg
    10981 acttcgatca gcgtgccctc ttcgcgctcc agcgccgcga ccagttccgg ttcgaattcc
    11041 gccgtgcggg taatgcgcac gccgacgtag ccataggcgc gtgccagcgc cgcgaagtcc
    11101 gggttggcca ggtcgctgcc gctgacgtgc cgcggatagt cacgctcctg gtgcatgcgg
    11161 atcgttccgt acatgccgtt gttgaggagt accacgattg tcctggcgcc gtgttgcacg
    11221 gcggtggcga gctcctggcc gttcatgagg aagtcgccat cgccggccat cgtcaggacc
    11281 gtccgtccac tgaccaggtt ggcggcgatg ccggccggaa cgccataccc catcgccccc
    11341 accgtcgggg ccagctgcgt cttgaatccc ttggccaatc cgtgatgctt gaaatagcgg
    11401 tgcatccagc tcgcgaagtt gccggctccg tttgtgagca cggcatcggc cggcaggtgc
    11461 ttgctgatca ccgcgacgat cgccggcatg ttgaccacgc cagcagccgt atccccctga
    11521 aggccggcga ggggctgcgg caccaggttg gcctcgtaat cggcgtgcgc ggcctcggtc
    11581 caggcggacc agggcacctc gggtggcgcc gcgagcgcct cgagcgagcg agcggccgcg
    11641 ttcatcgtcg cgttgatggc cacgtcggcc tggtacacgc ggttgagctc ctcggcgccg
    11701 gcgtggatat gcaccagttt ctgcctggcc ttcggtgcct gcagcagcgt gtagccgccg
    11761 gtggtcatct cgcccaggcg gggaccaacg gccaggacca ggtcgctttc cttgacgcgc
    11821 gccgccagcc tggggttcac gccgatgccg acatcgcccg catacagcgg gtggtgattg
    11881 tcgaacgtgt cctggaagcg gaaggcgttg cccaccggca gtttccagtt ctcggcgaag
    11941 cgctgcagcg cctgggccgc ctgcaccgtc cagccgccgc cgccggcgat cacgaatggc
    12001 tttgtcgctt gcagcagcag ttcgcgcact tcgcgcaagg agccgggatc gctccaggcc
    12061 tgcaccggtt ccacgcgcgg cagcgggcgg acagccgtgc gccgcaccag catgtcctcc
    12121 ggcagaacca gcacgaccgg cccgggcctg ccgttcatgg ccgtggcgaa ggcgcgcgcc
    12181 acgtattcgg gaatgcggtc cggctcgtcg atacgctcga cccgcttggc catgcccttg
    12241 gtgctggggc cgaagaagct ggtgtactcg acctcctgga aggcttcgcg atcgcgctgg
    12301 tcgctcgcca cgtcccccac gaacagtacc atcggagtcg agtcctggta cgccgtgtgc
    12361 acgccgatcg atgcgttcgt cgcaccggga ccgcgggtga cgaaacacac gcccggccgc
    12421 cctgtcagct tgccatgcgc ttcggccatg aacgccgcgc cgccttcctg gcgattgacg
    12481 atgaagcgga tccgttcgcg gtaagcatgg aagccatcga gtaccgccag atagctttct
    12541 ccgggaacgc cgaagacatg ggtgacacct tgggcgagca ggcattcaac caggagatgg
    12601 ccggcgattt gggcaggggc agtcgacatt cggcttgact tgattaacca atcggtgaat
    12661 tattacggca tgaccagaga catcgtcccc gccacggaag agcggcagcg cgacgccgac
    12721 cgctcccagg ccatcatctt agccgcggcg cgcgacgagt tcgcggagca cggactgggc
    12781 ggcgcgcgca tggaccggat cgcggagcgg gcgggactga acaagcgcct gatctactac
    12841 tacttcgagg acaaggaaac gctgttccag gccgtcctgg agcaggctta ccgcgacatc
    12901 cgcgaacagg agcgcaagct gcgcctgctc gacctcaaac ccgccgacgc ggtgcggcgg
    12961 ctggtcgagt tcacctggaa ttactatctc tcgcaccccg aattcctcac cctgctcaac
    13021 agcgcgaacc ttcacaaggc gcggcacctg caggcgtcca agagggcgcg ggagatgaac
    13081 tcgccgctga tagaaatgct tgcggagatt ctcgaaaggg gccgcaagga cggcagcttc
    13141 aggggcggcg tcgatccgct gcagctgtat gtttccatcg ccggcctggc gtatttctac
    13201 ctgtccaaca gccacacgct ttccgcgatc ttcggacgcg atctgatgtc ctccaaggcc
    13261 aggaacgaac gcctgaccca catgtgcgac gtgatcctgg gctacgtcct gaggagctga
    13321 caagccgggc cgcccgctgg ggcttgcgct tggcggtgcg cgccctataa ttcaccatat
    13381 agttaattgc gaccgctttt tttcgcaaga aacggagtga acatgccgac actcagactg
    13441 ggaatcatca tgcacggcgt gaccggacgc atgggcatga accagcacct gatccgctcc
    13501 atcgtcgcca ttcgcgccca gggtggcgtg acgctgtcca gcggcgactt gtgtgtgccc
    13561 gacccgatcc tgatcggccg caacgccgag aagatcgccg cgctggcaaa ggcccatggc
    13621 atcgagcgct ggggcaccga cctggatcgg gccctcgcca acaaggacga caccgtcttc
    13681 ttcgacgccg gtaccacgca gatgaggcct tcgttgctgg cgaaggccat ccacgcgggc
    13741 aaacacgtgt actgcgaaaa gccgatcgcc accaacctga acgaagcggt ggagatcgcc
    13801 cggctggcgc aggcgtcggg gctcaagcac ggcgccgtgc aggacaagct cttcctgccc
    13861 ggcctgcgca agctcgacat gctgcgccgc gcgggatttt tcggtcgcat gctcgcggtg
    13921 cgcctggagt tcggctactg ggtgttcgag ggcgacctgc agccgatcca gcgcccgagc
    13981 tggaactacc gcaaggagga cggcggcggc atgatcctgg acatgatgtg ccattggcgc
    14041 tatgtgctgg acaacctgtt cggcgaagtg aaatcggtct cctgcatagg cgccacgcac
    14101 attccgcagc gctgggacga gtccggcaag gcgtatgtcg cgaccgccga cgacgcggcc
    14161 tttgccactg ccgaactggt gggccacaac ggcgagcccg tggtggcgca gatgaacatg
    14221 tcctgggcca cgcgcgtgcg gcgcgacgac ct
//
LOCUS       DASWIY010000005        29016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1969, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000005 DASWIY010000000
VERSION     DASWIY010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 29016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29016
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /gene="acnA"
                     /locus_tag="VGA59_00165"
     CDS             <1..420
                     /gene="acnA"
                     /locus_tag="VGA59_00165"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484491.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX9718116.1"
                     /translation="AMKYMAEGRPTVVFAGEEYGTGSSRDWAAKGTQLLGIKAVIARS
                     FERIHRSNLVGMGVLPLQFNGGDTWQSLHLNGEELVDVIPDAQLRPQSDATLVVTRAD
                     GSRQEVPLTLRIDTPIEVDYYKHGGILPFVLRQLLAA"
     gene            491..1684
                     /locus_tag="VGA59_00170"
     CDS             491..1684
                     /locus_tag="VGA59_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9718117.1"
                     /translation="MTRQVLIAGGGMGGLAAAVACIRAGWDARLYEQAAQFSEVGAGI
                     QLGPNATRILDDWGLDGALSAVAAFPQRLRVRSALDGTQLGLMRLGSAFTKRYGAPYA
                     TVHRADLQALLLDAARRAGVYLKLSSPVTAVLPFGDAVGLRIGEGREVEGDALVGADG
                     LWSVVRPHVYGDGPPAFTGHLAYRALTAQRDLPEALRSADVTVWLGARLHVVAYPVRC
                     GEQLNVVAIVEGTVPGPAQDWDQCAVAAELQAAIAAQCTQLRDLVLAMPHWRLWALHD
                     RPPMGSADEMVRGRVVLVGDAAHPMRPYFAQGAGMAIEDAAELGRAFAMAAKAGVDVP
                     LALRRYALNRWERCARVQARSRRNGRIFHATGLMQWGRDLSLRLLGEHLLDQQWLYRG
                     PAEQH"
     gene            complement(1726..2595)
                     /gene="kynA"
                     /locus_tag="VGA59_00175"
     CDS             complement(1726..2595)
                     /gene="kynA"
                     /locus_tag="VGA59_00175"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HEX9718118.1"
                     /translation="MNNDKPAPTLPAAAEAIVHQENALHDFSQSMSYADYLQLDAILH
                     AQKPLSPDHNEMLFIIQHQTSELWMKLMLHELRAAIANVAGDELGNAFKMLARVSRIM
                     EQLVHAWDVLATMTPPEYSAIRPYLGTSSGFQSAQYRCIEFALGNKNAAMLKTHAHRP
                     DLLAQVQAAYEAPSLYDEALRLLSRRGLAIPPGYTQRDWTQPYVESAQVEQAWLVVYR
                     DPKHHWDLYQLGEELTDLEDAFRLWRFRHVTTVERVIGFKRGTGGTGGVSYLRKMLDV
                     VLFPEIWKLRTDL"
     gene            complement(2745..4061)
                     /gene="kynU"
                     /locus_tag="VGA59_00180"
     CDS             complement(2745..4061)
                     /gene="kynU"
                     /locus_tag="VGA59_00180"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEX9718119.1"
                     /translation="MTTLQDCRALDAQDPLRELRGLFTLPGGVIYLDGNSLGVLPKAT
                     AARVAQAVTEEWGQGLIRSWNSAGWFSLPQRLGDKIARLVGAQAGEVVATDSTSINLY
                     KVLSAALRHAAQADARRKVVVSERSNFPTDLYIAESLCKERGYSLRLVEPDGIEAALD
                     DGAAALMLTHVNYRTGAMHDMARLTAAAHRKGALAVWDLAHSAGAVPVDLHGAGADFA
                     VGCGYKYLNGGPGAPAFVWVHPRHTDRFEQPLAGWWSHAAPFEFAPGYRPAPGIARYL
                     CGTQPILSMVALECGLDTLLAAEPIGGMAALRRKSLALTDLFIQLVEERCAGHGLGLA
                     TPREQDRRGSQVCFTRLDPQQPRGSSGAYAIVQALIGRGVIGDYRAGDPPASAGEAGT
                     QDILRFGFTPLYLGFEDVWRAVEQLRQVIESGEWRRPEFNQKHAVT"
     gene            complement(4058..4684)
                     /gene="kynB"
                     /locus_tag="VGA59_00185"
     CDS             complement(4058..4684)
                     /gene="kynB"
                     /locus_tag="VGA59_00185"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HEX9718120.1"
                     /translation="MKKLWDISPPVAPGSPVFPGDSPYEQEWSATLTPGNPVNVSRIT
                     LSPHVGAHADAPLHYDPAGSSIGAVDLSPYLGRCRVIHAIAKGPLVRWVHLEHAAHEL
                     PARVLVRTYERMPADRWDPELAAYAPEAIEHLAALGVKLVGIDTASIDPADSKTLDSH
                     QAIRRLGLRVLENLVLDDVPEGDYELIALPLKLMTADASPVRAVLREL"
     gene            4892..5242
                     /locus_tag="VGA59_00190"
     CDS             4892..5242
                     /locus_tag="VGA59_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718121.1"
                     /translation="MELQTARQILDTLAQGIHPVTGEAMPDDSPYNAPAVIRALFAVS
                     QALSEKPARPKREPPPNAGKAWDPEEDARLDAAYAQGIDVKQLAQELGRSSWAVESRL
                     VKLGKLPARPGMRL"
     gene            complement(5266..6213)
                     /gene="argF"
                     /locus_tag="VGA59_00195"
     CDS             complement(5266..6213)
                     /gene="argF"
                     /locus_tag="VGA59_00195"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX9718122.1"
                     /translation="MTLQHYLQFSDLAADEYAWLFRRAAIIKKKFKAYEKYHPLADRT
                     LAMIFEKASTRTRVSFEAGMYQLGGSVVHLTTGDSQLGRAEPIEDSAKVISRMVDLVM
                     IRTFGQDKIERFAGHSRVPVINGLTNEFHPCQILADLFTLLEHRGTDSQGLPDPAFLK
                     GKTVAWVGDGNNMANTWLQAAELLDFRVHVSTPTGYEVDQTVAGIRSSDSYKVFKDPM
                     DACRGADLVTTDVWTSMGYEAENEARKKVFADWCVDTEMMGAAKPDALFMHCLPAHRG
                     EEVEAEVIDGPQSVVWDEAENRMHVQKALMEYLLLGRVG"
     gene            complement(6221..7414)
                     /locus_tag="VGA59_00200"
     CDS             complement(6221..7414)
                     /locus_tag="VGA59_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9718123.1"
                     /translation="MAFIEAASPHTMNTYGRLPIALSHGQGVRVWDVNGKEYLDALAG
                     IAVNTLGHNHPKLVPALRDQIGRIIHSCNYYHVPDQERLAATLAELSGLTNAFFCSTG
                     LEANEAAIKLARKFGHDKGIERPEIVVYENAFHGRSIATLSATGNEKVQQGFGPLVEG
                     FIRVPLNDMAALKQATEGNPNVVAVFFETIQGEGGINPMRIEYLQQVRQLCDQRDWLL
                     MIDEVQCGMGRTGKWFAHQWAGIKPDVMPLAKGLGSGVPVGAVVAGPKAADIFQPGNH
                     GTTFGGNPLAMRAGVETIRIMREDGLLDNAARVGAHLKGALARELGSLKGVTEIRGQG
                     LMLGIELARNCTVLTSRAAEAGLLISVTADNVIRLLPPLIMTEGEADEVAALLVPLVK
                     KFLAQ"
     gene            complement(7741..8043)
                     /gene="rpsT"
                     /locus_tag="VGA59_00205"
     CDS             complement(7741..8043)
                     /gene="rpsT"
                     /locus_tag="VGA59_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX9718124.1"
                     /translation="MASAKPKKKNPRLASGRKRARQDVKLNAANTSLRSKYRTAVKNV
                     EKAVAAGDKAKAAEAFVKAQSIVDMVADKQIFHKNKAARDKSRLSAKVKALAQAAA"
     gene            8247..9812
                     /gene="murJ"
                     /locus_tag="VGA59_00210"
     CDS             8247..9812
                     /gene="murJ"
                     /locus_tag="VGA59_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX9718125.1"
                     /translation="MTLFKAASTVSLLTLASRVTGLVRDLLMAATFGATALTDAFNVA
                     FRIPNLFRRLFGEGAFSQAFVPALAHSRERDGEEATHRLIDSVATVLAWALLATCVLG
                     VAGAPILVWALASGFNRHPQGFEAAVLMTRWMFPYIGFMSLVALSAGVLNTWKRFAVP
                     ASTPVLLNLSMIGAAWLGAPWFASMGIQPIYAMAGGVMLGGLLQLGVQIPVLHRLGLL
                     PRVGLGWTALRAAWADPGARQVARLMVPALLGVGVAQISILINTQIASWLAPGSVTWL
                     GYADRLMEFPTAMLGVALGVVLMPQLASARASNDPDRYSAMLDWGLRLVVLFAVPSSV
                     ALLLFAKPLVATLFHYGAFSDGDVSQVALALVGYGTGLVGLVAIKVLAPGFYASQDMR
                     TPVRIAIMVLVITQLLNIALVPLLKHAGLALSIGLGALINALSLLIGLIRRGSYRPAP
                     GWGVFALQVFAASALLAVFLMWAAGSFSWVAMRSHSLQRVGLMAAMLAASAAIYFVAI
                     WAAGLKLRQFVTR"
     gene            9871..10722
                     /locus_tag="VGA59_00215"
     CDS             9871..10722
                     /locus_tag="VGA59_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9718126.1"
                     /translation="MLLNLTAPTPLEYFSSLVQSDDHFPLLEAAACLAQDEYPELDVQ
                     QVLGDVDQLFSRLKRRLPADAAPLQRLRMLNQYFFRDLSFGGNVNDYCDPDNSYLNVV
                     LRTRRGMQISLAVLWIELAQGLGLHARGVCFPGHFLVKVNLPKGQVVIDPFTGQSLSR
                     EELAERLEPYKRRSGLVDEFEVPLGLYLQAAPARDIIARMLRNLKEIHRAQEDWQRLI
                     AVQDRLVILLPDAWAEYRDRGLAHAEQGHSALAVTDLETYLVHAQDALDIDVIADRVA
                     DLRRAKN"
     gene            complement(10841..11407)
                     /locus_tag="VGA59_00220"
     CDS             complement(10841..11407)
                     /locus_tag="VGA59_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX9718127.1"
                     /translation="MKPCLVLIDAQESFRHRPYFTERDLPGYLAAQNALIEACRKRGV
                     PIVRIFHVDGPKTAQNPFALESGLVRPLEGLAPFEAAATFHKSRHSALVGTGLDVWLT
                     QNGIGRLIISGIRTEQCCETTARHASDLGFQVDYVTDATLTFDMKHEDGTPLPAADIK
                     ARTAAVLKDRFARICSVPQVLDRLATPE"
     gene            complement(11502..12698)
                     /locus_tag="VGA59_00225"
     CDS             complement(11502..12698)
                     /locus_tag="VGA59_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9718128.1"
                     /translation="MNARARPTSRLPAGIWALGFVSLLMDVSSELIHSLLPVFMVTTL
                     GISVLVVGLIEGAAEATALVVKVFSGVLSDYWGRRKPLAVLGYALGAISKPLFALATT
                     SGLVLSARLIDRVGKGIRGAPRDALIADITPAANRGAAFGLRQALDTVGAFLGPSLAI
                     GLMLLWANDFRAVFWVAVIPGFLSVALLVFAVREPPRPAGERRGNPVRRENLRRLGRA
                     YWWVVGVGAVFTLARFSEAFLVLRALQGGLAMAWTPLVMIAMSLVYAIGAYPFGKLSD
                     AMSHRTLLAWGLAVLIAADLLLAAGGAGWLLWGGVGLWGLHMAMTQGLLAAMVAQAAP
                     ADLRGTAFGFFNLMSGLALLLASGLAGLLWDQWGAPVTFLAGAAFSAAALSVLALRPR
                     PSPKMS"
     gene            complement(12709..13740)
                     /gene="purM"
                     /locus_tag="VGA59_00230"
     CDS             complement(12709..13740)
                     /gene="purM"
                     /locus_tag="VGA59_00230"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX9718129.1"
                     /translation="MSQSPLSYKDAGVDIDAGDALVERIKPLARKTMREGVLAGIGGF
                     GALFEVPKRYREPVLVSGTDGVGTKLKLAFEWNMHDTVGIDLVAMSVNDVLVQGAEPL
                     FFLDYFACGKLDVETAAAVVGGIATGCELSGCALIGGETAEMPGMYPPGEYDLAGFAV
                     GAVEKSKILTGAGVTPGDVVLGLASSGAHSNGFSLVRKCIERAAGKLPATLDGKPFRQ
                     AIMEPTRLYVKSVLAALAAHPIKALAHITGGGLLENIPRVLPAGTAAHLKKGSWPRTE
                     LFAWLQQTAAISDHEMNRTFNNGIGMVVVIDAANAQAVAATLRIYGETVYEIGVIAAA
                     DAGAAVVLA"
     gene            13876..14946
                     /locus_tag="VGA59_00235"
     CDS             13876..14946
                     /locus_tag="VGA59_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9718130.1"
                     /translation="MQFTPTQKHAAAWLLIALLAALALWSLGRVLTPFVVAAVLAYAL
                     TPLVDRLDDLGRGRMPRFVAVAIVELLLIVTLVLLMLIVVPVLAKEAPLIREQLPFLF
                     DRLNAALAPWLEQLGITIALDLASLKAQLLKYLSANFEDMVGSLFSSLRVGGGVALIV
                     IGNAVLIPVALFYLLLDWKLLVHRVLELVPPRARPGVDSFATEADQVLGQYLRGQLLV
                     MVTMAIFYSTGLSLFGLDLALPIGVFTGLAMFVPYVGFGIGLVLAILAGILQFASIKA
                     LVMVAVVYGTGQVIEGFYITPRLVGRRIGLHPLLVIFALLAFAQLFGFVGVLVALPAS
                     AVLLVAIRRLRAGYIGSNLYQS"
     gene            14946..15620
                     /gene="hda"
                     /locus_tag="VGA59_00240"
     CDS             14946..15620
                     /gene="hda"
                     /locus_tag="VGA59_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEX9718131.1"
                     /translation="MKQIALDIGLARAPTLASFCAGPNEAALKHLQLWAGSPTRSPVP
                     TYLWGAAGSGKTHLLKAVAQQLREHGAAAGWLDAALLEPPDFNESWAVVLLDDVHLYT
                     AVQQHAAFNWFVNAQSLQRGVLAAGALPPADLKLREDLRTRLGWGHVFQLQVLSEPER
                     RAVLRQAADARGVFLGDDVMDFMLTRFSRDLGSLMQLLDQLDGYALQTKRAITIPLIR
                     SMLENE"
     gene            15617..16297
                     /locus_tag="VGA59_00245"
     CDS             15617..16297
                     /locus_tag="VGA59_00245"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9718132.1"
                     /translation="MSVTKTRLALFDLDHTLLPIDSDYAWGVFTQDIGWTDPVVFKER
                     NDEFFEHYKAGTLDIHDYVRFATEAVRLRGPAEAAVAHARFMQTVIRPAVRAEALDLV
                     RRHRDAGELPVIVTATNEFITRPIAGEFGVEELIAVQLERGPDGWITGEIVGVPSLRE
                     GKVERLGQWLAARGWDRGDVEATFYSDSMNDLPLLEAVDHPVATNPDARLRAVAVQRG
                     WRILDLFK"
     gene            16294..17889
                     /gene="pcnB"
                     /locus_tag="VGA59_00250"
     CDS             16294..17889
                     /gene="pcnB"
                     /locus_tag="VGA59_00250"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX9718133.1"
                     /translation="MIKKFIDKLLGTSLGGKGGKPSFGKRQEVGPQEHGIDPSLVDDR
                     ALNVVHTLKEAGFEAYIVGGAVRDLLVGLRPKDFDVATSATPEQVKGQFRRAFIIGRR
                     FRIVHVVFGRGREHEVIEVSTFRAYMDNVAAEQVAGNEKTSRSELAGMKHAVDSTGRV
                     LRDNVWGPQAEDATRRDFTINAMYYDPETQTVVDYHGGIKDAKKRTIRMIGDPLARYR
                     EDPVRIIRAVRFAAKLNPLGFSLEAKTAAPLVQCQALLADVPQSRLFDEMLKLLQTGH
                     SLACIEQLKALGMARGIYPLLDLVVERAEEPFVKAALQDTDRRVGENKAVAPSFLLAC
                     VLWSDVRDGWAQRLAQKHHPFPALQDAIDDVFNSRIGDVSGRGKLGADMREIWMMQPR
                     FEKRSGNTPFSLVEQPRFRAGFDFMRLRADVGEVDVVLADWWQEFSQAGDAMREDLVA
                     QLREEQHKGQRRVRTPRPAERAADKSPHAGGAAPEPVPEREAVEAREAGPSDAPRKRR
                     RRRRKPSGEGGAGPAAEPAEPLQ"
     gene            17916..18380
                     /gene="folK"
                     /locus_tag="VGA59_00255"
     CDS             17916..18380
                     /gene="folK"
                     /locus_tag="VGA59_00255"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX9718134.1"
                     /translation="MRDAVPAYVGLGANLGDAARTVREAIARIAELPQTAVTRQSSLY
                     RTAPVDAAGPDYINAVAEIFTRLTAPELLAQLKGLERAAGRERPYRNAPRTLDLDLLR
                     FGDAQIDSAVLQVPHPRMHARAFVLVPLAEIAPHLVAPAQLAAVADQRLSRL"
     gene            18384..20222
                     /locus_tag="VGA59_00260"
     CDS             18384..20222
                     /locus_tag="VGA59_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718135.1"
                     /translation="MKLFTLWLPKYSFSLAVMMAFLAIILTPLNQLGLSQYNIEKTLP
                     ENKIEFPGRNKNAFNVGDRLPIYRFNPEWEKPIGEVEVLAYDNGKWIAGFNPDTLRWP
                     MGNQGTIVRVDAQNVSLNVGQRVGVKAGDTWNIFDGRDNIGKIVIRTVHEFESVAQLV
                     QPLSNNSTLVGLQVSPYILATQVIVFDNLFIRILQITVLFTAFTLWVWSMFSETPANI
                     WFSLCQVLRDKISAAPTAVRFRFLVIIGVPICYGIGALVWNILCHINWYIHTAWLNQS
                     IEHYPAWGTTWMQILVAAAWYAVLFGMLRSPMSYLVERLRYKPLNFSWFPLGAKPYLI
                     WLLHLFVYYAFTNTLGQYAVANMQVMVNHAWPDFRAAEEPVISSVFLPWMDLFSANYS
                     SSKHLVDRFYELLVHMSTHAPVIADPVIGFLLLRLSMWTLTICVILCLYTYTVICILW
                     TRSAVRNIDFTFAGWVTAAVCYGPLLGDPLHQTIPSAHGLDPTYITDVWMWFVLVVES
                     LLNLLYMLSIFNLGLMFGMLVDKGVRTQGFYAVIRHPNYTLEALMFVVLGLMALSGPL
                     QWIAISVIFIKYWLRSEREDQFMSGSNRDYLTYKTQTPWKFIPGLY"
     gene            20227..20367
                     /locus_tag="VGA59_00265"
     CDS             20227..20367
                     /locus_tag="VGA59_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718136.1"
                     /translation="MVPAYAKLDFETIFRAQVADDAFVIGFHLPKDRPPFANLEAGEH
                     CE"
     gene            20480..20722
                     /gene="atpE"
                     /locus_tag="VGA59_00270"
     CDS             20480..20722
                     /gene="atpE"
                     /locus_tag="VGA59_00270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX9718137.1"
                     /translation="MSDAGLIAIACGLIVGLGAIGACLGIGTMGGRFIDGAVRQPELM
                     EPLQTKMFLLAGLIDANFLIGVGVAMMFVFANPFAK"
     gene            complement(20729..21205)
                     /locus_tag="VGA59_00275"
     CDS             complement(20729..21205)
                     /locus_tag="VGA59_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX9718138.1"
                     /translation="MALRPVVLPPQVPGSLWLGSMPGRFETWSAFESQARRAGLALVV
                     CLTPRSELAELSPAYHRSVAQGTVPFRWLNVPMPNFGVPEDPAAFRREVQQIAAALRQ
                     GDAVLLHCAAGLGRTGSTAACVLKALGLDSEEALQRVRDAGSNPQNARQSGVVDWF"
     gene            complement(21296..22573)
                     /locus_tag="VGA59_00280"
     CDS             complement(21296..22573)
                     /locus_tag="VGA59_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9718139.1"
                     /translation="MRRFLGALLVGMLAVLALVLLLNFWGEDKIHDAPPLTPPAGESV
                     ARGAYLVRVGNCRACHTERGGAAFAGGRAIETPFGAVYASNLTPHATGLGNWSASHFW
                     RAMHHGRSRDGRLLYPAFPYPHYTLVTRADADAIYDYLRSLAPVERANTPHSLRWPYS
                     TQTALAIWRALYFRPDAHEDHPGQSAEWNRGAYLVNGLGHCGACHTTRNVLGASSDMI
                     DLSGGLIPMQNWYAPSLASPAEAGVGDWDLEHTVRLLQAGVSPRGTVLGPMAEVVLQS
                     TQHLAPADLRAMAVYLKSLPQSKPHTQAAQGPRMISGEMAERGGKLYRDHCAQCHGDD
                     GEGVPGAYPALAGNRAVTLAVPANLVQVVLGGGFPPATAGNPRPFGMPPFATVLSDAQ
                     VAAVITHIRTSWGNRGTAVGEFTVSQQRGGSRL"
     gene            complement(22570..23340)
                     /locus_tag="VGA59_00285"
     CDS             complement(22570..23340)
                     /locus_tag="VGA59_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9718140.1"
                     /translation="MRRFNRTAARPAVRWWHSLDIVKSLIFLLVILAAACARARPVFE
                     DTMAQRTLACSACHGRQGRAGPDGYYPRIAGKPAGYLYNQLLNFRDGRRHYGLMTRLL
                     DPLSDAYLLEIAEHFAALDLPYPPPAPPTMPPATLQRGQTLARDGDAAKNIPACAQCH
                     GPALTGVLPNTPGLLGLSRDYLNSQLGAWRTGQRQAHAPDCMAAIARHLEPEDLTAVA
                     SWLAAQPLPASTRPAAALPRPPAIACGSAAKPAGRQQP"
     gene            complement(23340..23687)
                     /locus_tag="VGA59_00290"
     CDS             complement(23340..23687)
                     /locus_tag="VGA59_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9718141.1"
                     /translation="MTDDSGPAPLFSSDAPAARFDVRFEPGGPRFTASAGQPLLLAAE
                     QAGLQLPSSCRNGTCRTCMCRVLQGRVRYRIQWPGLLAEEKAEGWILLCIAHAESHLV
                     LSPPRNFRLPAGP"
     gene            23745..24260
                     /locus_tag="VGA59_00295"
     CDS             23745..24260
                     /locus_tag="VGA59_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9718142.1"
                     /translation="MSSYPLRPAVAGDAEQVAAVHASSSHAAYEAVAPEAHQSLPIER
                     RRAFWREAIEFGEPLVQVALEAGRVIGFAGYDRSRDPKSKATTGEIWAMYVQPSHWGR
                     GVGLALWDATREALVEEGCTEVTLWVPLCNDRALRFFELAGFKRELNTAKTAVLGGVK
                     LEEIRLRRGLD"
     gene            24280..25197
                     /locus_tag="VGA59_00300"
     CDS             24280..25197
                     /locus_tag="VGA59_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HEX9718143.1"
                     /translation="MASSLLALLDDIATILDDVSVLAKVAAKKTAGVLGDDLALNAQQ
                     VTGLAAERELPVVWAVAKGSLVNKAILVPAALAISAVAPWAVTPLLMVGGAFLCYEGF
                     EKLAHRFLHSRSYDEAHRQELEHALLNPDIDLRVVEKDKIKGAVRTDFILSAEIIAIT
                     LGTVADSAFAVQVGVLGGIAILMTIGVYGLVAGIVKLDDAGLYLSRKSQAIARSAGTA
                     ILSAAPWLMKGLSVAGTAAMFLVGGGILVHGVAPLHHAIERFAESAGAGFGALLPMLL
                     NALVGVVAGAVVLGVVTLAQRLARRRAAV"
     gene            25194..26360
                     /locus_tag="VGA59_00305"
     CDS             25194..26360
                     /locus_tag="VGA59_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9718144.1"
                     /translation="MSGPPQLHWTEGSGERSALWRSEAAVPPPKRVQVVDDTLSADAA
                     YRLASEGTALLWRSDFHNARQLLQAMARRADRKPRNPAAAPASPTEAFHLHRQAQGQR
                     SRILGRLLIALEGDYRIALPRAPDLRQACLEAWGPADGQPSVASLRELLGLVGAHEWR
                     KKGVEVPALGAPPGNRIHPHYGVFSPVRGEYLELVAKAPLPSKDLAFDIGTGTGVLAA
                     ILARRGVRRVVATDQDSRALACARENVARLGLQDKVEVRQADLFPPGTAPLVICNPPW
                     LPALPSSPLERAIYDEDSRMLRGFLAGLAAHLAPGGEGWLILSDLAEHLGLRSREDLM
                     GLIETSGLRVVARSDVRPGHPKTGDTRDALHAARAAEVTSLWRLVPLQTGQARL"
     gene            26454..26642
                     /locus_tag="VGA59_00310"
     CDS             26454..26642
                     /locus_tag="VGA59_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718145.1"
                     /translation="MPEPSQQPLPPAHPRPAAALVETTARWLDIDDDLALGCESADPS
                     LQIERWGQEVLPFPATSS"
     gene            26827..27108
                     /locus_tag="VGA59_00315"
     CDS             26827..27108
                     /locus_tag="VGA59_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718146.1"
                     /translation="MNATKKSLTTLAVAAALAGGVGLAIAQSTSTETPAAAGSSYGAT
                     QSSDTQSSGAGPSTAGTATLGGSTDSSSSTTMSNTDSTTEERVAQIDRN"
     gene            27190..27957
                     /locus_tag="VGA59_00320"
     CDS             27190..27957
                     /locus_tag="VGA59_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718147.1"
                     /translation="MNPLPTPRSASSEFLDSVQKGLLLGAAVLALVLPPNIGIVHRQA
                     YAPATHAPARMAPPPVRLADFAGRVPSPDVRHLANWAVQTRDHQNRSLVIVDKKGASI
                     YVLDPAGRLQGNAPVLLGSAVGDHTVPGIGDKPIDQVLPEERTTPAGRFVAEVGMNMR
                     GEDVVWVDYDSAVSLHRVLTTNPSERRLERLASPEVEDNRISYGCINLPRDFFETVLS
                     PAVRTAGAIIYVLPETRSVQQVFGSYDVEAATNLARR"
     gene            28166..28738
                     /locus_tag="VGA59_00325"
     CDS             28166..28738
                     /locus_tag="VGA59_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9718148.1"
                     /translation="MAEQIAAPDFGTHLASADARDVARLIFESADPAGLPFAIVDKKE
                     AQIYVFDAQGQLSGVSPALLGQARGDHSAPDVGRKAQTGSVPLNERTTPSGRFISRPG
                     RNLDGEHVVWVDYDAAFAIHRLRPGASQPQRVARLASATPHDNRASLGCVVVPETFYS
                     DVVQPLLGRTRAVIYVLPEERPARELFNAI"
BASE COUNT         4652 a         9439 c         9786 g         5139 t
ORIGIN      
        1 gcgatgaaat acatggccga gggccgtccg accgttgtct tcgccggcga ggagtacggc
       61 accggctcgt cgcgcgactg ggcagccaag ggcacgcagc tgctgggcat caaggccgtc
      121 atcgccagga gtttcgagcg cattcaccgc agcaacctgg taggcatggg cgtgttgccg
      181 ctgcagttca acggcggcga tacctggcag tcgctgcacc tcaatggcga ggagctggtc
      241 gacgtgatcc ccgatgcgca attgcggccg caaagcgatg cgacgctggt ggtcacgcgc
      301 gccgacggca gccgccagga agtcccgctg acactgcgca tcgatacgcc gatcgaggtc
      361 gactactaca agcacggcgg catccttcct ttcgtgctca ggcagctgtt ggccgcatga
      421 gcgcaaaagg cctgccgatg ggaatcgcca cgcgggcgca tcgcgcggtg tttcgtcgac
      481 tgctcgcggc gtgacgcggc aagtcctcat cgccgggggc ggcatgggtg gactggccgc
      541 ggccgtggcc tgcatccggg cgggctggga cgcgcggctg tacgagcagg ccgcgcagtt
      601 cagcgaggtc ggagccggca tccagctggg tcccaatgcg acgcgcattc tggacgactg
      661 gggcctggac ggggcgctgt ccgccgtggc ggcgttcccg cagcggctgc gcgtgcgcag
      721 cgcgctcgac ggtacgcagc tgggcctgat gcgcctgggc tcggcgttca ccaagcgcta
      781 cggcgcgcct tacgccacag tgcaccgtgc cgacttgcag gcgctgctgc tggatgcggc
      841 gcggcgagcc ggcgtctatc tcaagctctc gtcaccggtc accgcggtgc tgcctttcgg
      901 cgatgcggtc gggctgcgca tcggcgaagg ccgtgaagtg gaaggcgacg cgctggtcgg
      961 cgcggacggc ctttggagcg tggtgcgccc gcacgtttat ggcgacgggc cgccggcatt
     1021 caccggccac ctggcttatc gcgcgctcac tgcgcaacgc gacctgcccg aggccttgcg
     1081 cagcgcggat gtcacggtct ggctcggggc gcggctgcat gtggtggcct accccgtgcg
     1141 atgcggcgag cagctgaatg tggtggcgat cgtggagggc acggtgcccg gcccggccca
     1201 ggactgggac cagtgcgcgg tcgctgccga gctgcaagcc gcgatcgcag cgcaatgcac
     1261 ccagctgcgc gacctggtgc tcgcgatgcc gcactggcgg ctgtgggcac tgcacgatcg
     1321 cccgccgatg ggaagtgccg acgagatggt gcgcgggcgt gtcgtgctgg tgggggacgc
     1381 cgcgcatccg atgcgtccgt atttcgccca gggcgcgggc atggccatcg aggatgcggc
     1441 ggaactgggc cgcgcgtttg ccatggccgc gaaggccggc gtcgacgtgc cgctggcgct
     1501 gcgccgctat gcgctcaacc gctgggagcg ctgcgcgcgg gtgcaggccc gctcccggcg
     1561 caatggccgg atattccacg ccaccggcct gatgcagtgg ggccgcgacc tgtcgctgcg
     1621 gctgctgggc gaacacctgc tcgatcagca gtggctgtac cgcggcccgg ctgaacaaca
     1681 ttagccgcgc gcggtccccc ggcttgcctt tgcgttcaac gtggcctaca agtcggtgcg
     1741 cagcttccag atctccggaa acagcaccac gtccagcatc ttgcgcaggt agctcacgcc
     1801 gcccgtgcca ccggtcccgc gcttgaagcc gatcacgcgt tccacggtgg tgacatggcg
     1861 gaatcgccag aggcggaacg cgtcttcgag gtcggtgagc tcctcgccca gctggtacag
     1921 gtcccagtgg tgcttgggat cgcggtacac gacgagccag gcctgttcca cctgcgcgct
     1981 ttcgacgtag ggctgggtcc agtcgcgttg cgtgtacccc ggcggaatcg ccagcccgcg
     2041 ccgcgacagc aggcgcagcg cttcgtcgta aagcgacggc gcctcgtagg cggcctggac
     2101 ctgggccagc agatcgggcc ggtgcgcgtg ggtcttgagc atcgcggcgt tcttgttgcc
     2161 cagcgcgaac tcgatgcagc ggtactgcgc gctctggaaa ccgctcgaag tgccgaggta
     2221 ggggcggatg gcgctgtact ccgggggcgt catcgtggcc agcacgtccc acgcatggac
     2281 cagctgttcc atgatgcggc tgacccgcgc cagcatcttg aaggcgttgc cgagttcgtc
     2341 gcccgcgacg ttggcgatgg ccgcgcgcag ctcatgcagc atcagcttca tccacaactc
     2401 gctggtctgg tgctggatga tgaaaagcat ctcgttatgg tccggcgaga gcggcttctg
     2461 cgcgtgcagg atggcgtcca gctgcaggta gtccgcatag ctcatggact ggctgaagtc
     2521 gtgcagcgcg ttttcctggt gcacgatggc ttcggcggcg gcaggcagcg tcggggcggg
     2581 cttgtcgttg ttcacttcag tttcccatga agcagaactt cacccaggac ggatgaaagt
     2641 cgattctgca ggcgcgggcc accgcggaac cggctttgcc gggccgctgg tggcgccccc
     2701 tcgaggggga ggcgccgaag gcgcttcggg ggtgggcttt cacttcaggt caccgcgtgt
     2761 ttctggttga actcaggccg ccgccactcg cccgattcga tcacctgtcg cagctgttcc
     2821 accgctcgcc acacatcctc gaagcccaga tacagcggcg tgaagccgaa tcgcaggatg
     2881 tcctgtgttc ctgcctctcc cgcgcttgcc ggagggtctc cggcgcggta gtcgccgatc
     2941 acgccacgcc cgatgagcgc ctgcacgatc gcatacgcac ccgaactgcc gcggggctgc
     3001 tgcggatcga gccgcgtgaa gcagacctgc gagccgcgac ggtcctgctc gcgcggtgtc
     3061 gccaggccca ggccgtggcc ggcgcagcgc tcttccacca gctggatgaa caagtcggtc
     3121 aatgccaggg atttgcggcg cagcgcggcc atgccgccga tgggttcggc ggcgagcaaa
     3181 gtgtccaggc cgcattcgag cgcgaccatg ctcaggatcg gctgggtgcc gcaaaggtag
     3241 cgcgcaattc cgggcgcggg gcggtagccg ggcgcgaatt cgaacggcgc cgcatggctc
     3301 caccaacccg ccagcggctg ttcgaaccgg tccgtgtggc gcggatggac ccagacgaag
     3361 gccggcgcgc cggggccgcc gttgaggtat ttgtagccgc agccgaccgc gaagtcggcg
     3421 ccagcgccat gcaggtcgac cggtaccgcg cccgcgctgt gtgccaggtc ccagaccgcg
     3481 agcgcgccct tgcgatgcgc ggctgccgtg agacgggcca tgtcgtgcat cgcgcccgtg
     3541 cggtagttga catgggtgag catgagggcg gctgcgccat cgtcgagcgc cgcttcgatc
     3601 ccgtcgggtt cgaccagtcg cagcgagtag ccgcgttcct tgcaaagcga ttcggcgata
     3661 tacaggtcgg tcgggaaatt gctgcgctcg ctcacgacca ccttgcgccg cgcatccgcc
     3721 tgggcggcgt ggcgcaacgc ggcgctgagc accttgtaga ggttgatcga cgtgctgtcg
     3781 gtggccacca cctcgcccgc ctgcgcgccg accagccggg cgatcttgtc gcccaggcgc
     3841 tgcggcaggc tgaaccagcc ggcgctgttc caggagcgga tcaggccctg gccccattcc
     3901 tcggtgacgg cctgtgccac gcgcgccgcg gtcgctttgg gcaggacgcc cagcgaattg
     3961 ccgtccaggt agatcacccc cccgggcagc gtgaacaggc cgcgcaattc gcgcagcggg
     4021 tcttgcgcat ccagcgcacg gcagtcctgc agtgtcgtca tagttccctg agcaccgcgc
     4081 gtaccggcga tgcatcggcc gtcatcagtt tgagggggag ggcgatcagc tcgtaatcgc
     4141 cctcgggcac gtcgtcgagc acgaggtttt ccaacacgcg caacccgagg cgtcggatcg
     4201 cctggtggct gtccagcgtt ttgctgtccg ccgggtcgat gctcgccgtg tcgattccga
     4261 ccagcttgac gccaagcgcc gccaggtgct cgatggcttc gggcgcatag gccgcgagtt
     4321 cggggtccca acgatccgcc ggcattcgct cgtaggtgcg caccaggacc cgcgccggca
     4381 actcgtgcgc cgcgtgctcc agatgcaccc atcgcaccag cggtcccttg gcgatggcat
     4441 ggatcacgcg gcagcgaccg aggtaaggcg acagatccac cgcaccgatc gacgagccgg
     4501 ccgggtcgta gtgcagcggc gcatcggcat gcgcgcccac atgcggcgac agggtgatac
     4561 ggctgacatt gacgggatta ccgggtgtga gcgtcgccga ccattcctgc tcgtagggcg
     4621 agtcgccggg aaagaccggc gagccggggg ccaccggcgg ggaaatgtcc cagagctttt
     4681 tcatgcagaa aagtgtgcgc cgctgccaca agccgtggtg tcggtttatt ccaacaaacg
     4741 ccgcaagtcg ttgaagctta aagcaatcgc gcctgcgatg gggcccagcg tcaagtccgc
     4801 gtcggcagcc cgtcaacctg ggcctgggct ggcgcgttgc ggaggaactg tatgaaaata
     4861 cagttaaccc aacttctcat caggaggaac catggaactg caaaccgccc gccagatcct
     4921 cgacaccctg gcccagggca ttcacccggt cacgggcgag gcgatgcccg acgacagccc
     4981 ctacaacgct ccagcggtga tccgcgcgct gttcgcagtg tcgcaggcgc taagcgagaa
     5041 gcccgcgcgg ccgaagcgcg agccgccgcc caatgcgggc aaagcatggg atcctgaaga
     5101 agacgcccgg ctggacgcgg cttatgcgca gggtatcgac gtgaagcagt tggcgcagga
     5161 actgggccgc agctcctggg ccgtggagtc gcggctggtc aagctcggca agctgccggc
     5221 gcgccccggc atgcggcttt gagtgaaggg tggcctggcc gcccttcagc caaccctgcc
     5281 cagcaaaagg tactccatga gtgccttttg cacatgcatt ctgttttccg cctcgtccca
     5341 cacgactgac tgaggaccgt cgatcacctc ggcttcgacc tcctcgccgc gatgagccgg
     5401 caggcaatgc atgaacagcg cgtcgggttt ggcggcgccc atcatctcgg tgtcgacgca
     5461 ccagtcggca aagaccttct tgcgcgcctc gttctcggcc tcgtagccca tgctggtcca
     5521 gacgtcggtg gtgacgaggt ccgcgccgcg gcaggcatcc atcgggtcct tgaacacctt
     5581 gtagctgtcg ctgctgcgaa tgccggccac ggtctggtcc acctcatagc cggtcggcgt
     5641 gctcacatgc accctgaaat cgagcagctc ggctgcctgc agccaggtgt tggccatgtt
     5701 gttgccgtct cccacccagg ccacggtctt gcccttgagg aaggccgggt ccggcaggcc
     5761 ttgcgaatcg gtgccacggt gctccagcaa ggtgaacagg tcggccagga tctggcaggg
     5821 gtggaactcg ttggtgaggc cattgatgac cggcacccgc gaatgcccgg cgaagcgctc
     5881 gatcttgtcc tgtccgaagg tgcggatcat caccaggtcc accatgcggc tgatcacctt
     5941 ggcgctgtcc tcgatcggct cggcgcgccc gagctggctg tcgccggtgg tcaggtgcac
     6001 cacgctgccg cccagctggt acatgccggc ctcgaaactg acgcgggtgc gcgtggaggc
     6061 tttctcgaaa atcatcgcca gcgtgcggtc ggccagcggg tgatatttct cgtaagcctt
     6121 gaatttcttc ttgatgatgg ccgcgcgccg gaacagccag gcatattcgt cggccgccag
     6181 atcgctgaac tgaaggtaat gttggagggt caaggcggcc tcactgcgcg aggaacttct
     6241 tcaccagggg cactagcagc gccgccactt cgtcggcttc gccctcggtc atgatcagcg
     6301 ggggcagcag gcggatcacg ttgtcggcgg tcacgctgat cagcaaaccc gcttcggcgg
     6361 ctcggctggt gagaacggtg cagttcctgg ccagctcaat gcccagcatc aggccctgcc
     6421 cgcgaatttc ggtcacgccc ttgaggctcc caagctcgcg agccaatgcg cctttcaggt
     6481 gcgcgcccac cctggccgcg ttgtccagca ggccatcctc gcgcatgatg cggatcgttt
     6541 cgacgccggc ccgcatcgcc agcggattgc cgccgaaggt ggttccgtgg ttgcccggct
     6601 ggaaaatatc cgccgccttc ggcccggcca ccacggcgcc gaccggcacg cccgagccca
     6661 ggcccttggc cagcggcatc acgtcgggct tgatcccggc ccattggtgc gcgaaccatt
     6721 tgccggtgcg gcccatgccg cactgcactt cgtcgatcat cagcagccag tcccgctggt
     6781 cgcacagctg gcgcacctgc tgcaggtatt cgatgcgcat ggggttgatg ccgccttcgc
     6841 cctggatggt ctcgaagaag acggcgacca cgttcggatt gccctcggtg gcctgcttca
     6901 gtgccgccat gtcgttgagc ggtacgcgga tgaatccttc aacgaggggg ccgaagccct
     6961 gctgcacttt ctcgttgccg gtggccgaca gcgtggcgat gctgcggccg tggaacgcgt
     7021 tctcatagac cacgatctcc ggccgctcga tgcccttgtc gtggccgaac ttgcgcgcca
     7081 gcttgatcgc cgcctcgttg gcttccagtc cggtggagca gaagaacgcg ttggtcaggc
     7141 ccgacagctc ggccagcgtc gccgccagcc gttcctggtc gggcacgtgg taatagttgc
     7201 agctgtggat gatcctgccg atctggtcgc gcagggccgg caccagcttg gggtggttat
     7261 ggccaagggt attgaccgcg atccccgcga gcgcgtcgag gtactccttg ccgttgacgt
     7321 cccacacgcg caccccctgg ccatgcgaga gcgcgatcgg cagccggcca taggtgttca
     7381 tggtgtgggg cgaagccgcc tcgataaaag ccattgccat ctccataaaa gcaatcggct
     7441 caacgaattg agccgctgag gcccgatttt aggcgcgaag accgcgtgcg ctgcttgcca
     7501 attgcgcatc acagcgatgc gcggcacggc cgtccggggc cgatcaagtc ttgcatagga
     7561 taaacaacaa cctcccgatg gccggggcct gcccgttgcc cgagcccgcg cgcgaagcgg
     7621 tcgtggcctg aacgcgggct tgcgccaaca agaaagccgt cttgcgacgg ctttcttgct
     7681 ctttgctggc agcgcacccg tttcaaacgg cggccgaagc cgggctggtt gcctgatacg
     7741 tcaggcggcg gcttgggcca gggctttcac cttggccgaa aggcggctct tgtcgcgagc
     7801 ggccttgttc ttgtggaaga tctgcttgtc ggcgaccatg tcgacgatgc tttgcgcctt
     7861 gacgaaagct tcggccgcct tggccttgtc gccggcggcc acggccttct cgacgttctt
     7921 gacggcagtg cggtacttgg agcgcaggga cgtgttggct gcgttgagct tcacgtcctg
     7981 gcgagcgcgt ttacggcccg acgcgaggcg cgggttcttt ttcttgggtt tggctgatgc
     8041 cattgatgtg gttccttgtg tctgaggatg atgccagcaa agcctgccat tatatccccc
     8101 ggcgctgttg caggcgacgc cgggatggcg tgggcgcgaa gcaccgctcg cagccgcgag
     8161 aaagtcacgg gccagcggat ccgggcgctc aacagggcag tgggagacgg cggcactata
     8221 aactcggcac cgcaccagcc ccgccaatga cccttttcaa agccgcttcc accgtttccc
     8281 tgctgaccct ggcgtcccgt gtcaccggcc tggtgcgcga cctgctgatg gcggcgacgt
     8341 tcggggccac ggcgctgacc gatgcgttca acgtcgcctt ccgcataccc aacctgtttc
     8401 gccgcctctt cggcgaaggc gcgttcagcc aggccttcgt gccggccctg gcccattcca
     8461 gggaaaggga cggcgaggag gcaacgcacc gtctgatcga cagcgtcgcc accgtcctgg
     8521 catgggcgct gctggcgacc tgtgtcctgg gtgtcgcggg cgcgcccatt ctggtgtggg
     8581 cgctggccag cggcttcaac cggcacccgc aaggcttcga ggcggccgtg ctgatgacgc
     8641 gctggatgtt tccctacata ggcttcatgt cgctggtggc gctgtcggcc ggcgtgctca
     8701 acacctggaa gcgcttcgcg gtgccggcgt cgacgcccgt gctgctgaac ctctccatga
     8761 tcggggcggc ctggctgggc gcgccctggt tcgcctccat ggggatccag ccgatctatg
     8821 ccatggccgg cggcgtgatg ttgggcggcc tgttgcagtt gggcgtccag attccagtgc
     8881 tgcaccggct gggcctgctg ccgcgcgtgg gccttggatg gacggcgttg cgtgcggcct
     8941 gggccgaccc cggtgcgcgc caggtggcgc ggctgatggt gcccgcgctg ctgggcgtgg
     9001 gagtcgcgca gatttccatc ctcatcaata cgcagatcgc gtcctggctg gcgcccggaa
     9061 gcgtgacgtg gctgggctac gccgaccggc tgatggagtt tcccaccgcc atgctgggtg
     9121 tggccctggg cgtggtgctg atgccccagc tcgcgagcgc ccgggccagc aacgaccccg
     9181 accgctattc ggcgatgctc gactggggcc tgcggctggt cgtgttgttt gccgtgccct
     9241 cctcggtcgc gctgctgctg ttcgccaagc cgctggtggc caccttgttt cactatggtg
     9301 ctttcagcga cggcgacgtg tcccaggtgg cgctggcgct ggtgggctac ggcacgggcc
     9361 tggtcggctt ggtggccatc aaggtgctgg cgcccggttt ctacgcgagc caggacatga
     9421 ggacgccggt ccggatcgcg atcatggtgc tggtgatcac gcagctgctc aacattgccc
     9481 tggtgccgct gctcaagcac gccggcctgg ctttgtcgat cggcctgggc gccctgatca
     9541 acgcgctgag cttgctcatc ggcctgatca ggcggggcag ctaccggccg gcgccgggct
     9601 ggggtgtgtt cgcgctgcag gtcttcgcgg ccagcgcgct gctcgcggtt ttcctgatgt
     9661 gggcggcggg cagtttctcg tgggtcgcga tgcgttccca cagcttgcag cgcgtcggcc
     9721 tgatggctgc gatgctggcc gcctcggccg ccatttactt tgtggcgatc tgggccgcgg
     9781 ggttgaagct gcggcagttt gtcacgcgtt gacgccgcgc gcatcggacg ccggcttgac
     9841 ggttgcgggc ggctccatta caaagaactc atgctgctga atctgaccgc cccgacgccg
     9901 ctcgagtact tcagctccct ggtgcaaagc gacgatcatt tccccctgct ggaagcggcg
     9961 gcctgcctgg cccaggacga gtatccggag ctggacgtgc agcaggtgct gggcgatgtg
    10021 gaccagctgt tttcgcgcct gaagcgccgc ctgcccgccg atgcggcgcc gctgcagcgg
    10081 ttgcggatgc tcaaccagta cttctttcgc gacctgagct tcggcggcaa cgtcaacgac
    10141 tactgcgacc ccgacaacag ctacctcaac gtggtgctgc gcacccggcg cgggatgcag
    10201 atctcgctgg ccgtgctgtg gatcgaactg gcgcagggcc tgggcctgca tgcgcggggc
    10261 gtgtgcttcc ccggccattt cctggtcaag gtgaacctgc ccaagggcca ggtggtgatc
    10321 gatcccttca cgggccagtc gctgtcgcgc gaggaactgg ccgagcggct cgagccgtac
    10381 aagcggcgca gcggcctggt cgacgagttc gaggtgccgc tgggcctgta cctgcaggcg
    10441 gcgcccgcgc gcgacatcat tgcccgcatg ctgcgcaacc tgaaggagat ccatcgcgcc
    10501 caggaggatt ggcagcggct gatcgccgtg caggaccggc tggtcatcct gctgcccgat
    10561 gcgtgggccg aataccgcga ccgcggcctg gcccatgcgg agcagggcca cagcgccctg
    10621 gccgtcaccg acctggaaac ctacctggtc catgcgcagg atgcgctgga catcgacgtg
    10681 atcgccgacc gcgtggccga cctgcgccgg gccaagaatt gagcggcgag cacggccggt
    10741 ccggtcgtga cgccggcgct ggcgaggatg aggctgcccg gaagcagcac gaagaacagc
    10801 gggatcggcg gccggttcac atcagcggca gcaagcggcg ctactcgggt gtggcgaggc
    10861 ggtccagcac ctgcggcacc gagcagatcc tggcgaagcg gtccttgagc accgcggctg
    10921 ttcgtgcctt gatgtcggcc gccggcagcg gcgtcccgtc ctcgtgcttc atgtcgaaag
    10981 tcagcgtggc gtcggtcaca tagtcgacct ggaagcccag gtcggatgca tggcgcgccg
    11041 tggtctcgca gcattgctcg gtacggatgc cgctgatgat gagcctgccg atgccgttct
    11101 gggtgagcca cacatccagg ccggtgccga ccagcgcgct gtggcgagac ttgtggaagg
    11161 tagccgctgc ctcgaacggc gccaggccct ccagcgggcg caccaggccc gattcgaggg
    11221 cgaacgggtt ctgcgcggtc ttgggaccgt cgacatggaa gatgcgaacg atgggaacgc
    11281 cacgcttgcg gcaagcctcg atcaacgcgt tctgcgccgc aagatacccg ggcaggtcac
    11341 gctcggtgaa gtacgggcga tggcgaaagg attcctgggc gtcgatcaag acaagacatg
    11401 gcttcatggc gggcacctct tcaggtagcg tggtggaagt accgtatttt ctgccagcgg
    11461 cgcggaaccg gccattcatt gccggtccgc tttcgatcag ttcaggacat tttcggcgat
    11521 gggcgcgggc gcagcgccag gaccgacaag gccgctgcgc tgaaagccgc gccggccagg
    11581 aaggtgacgg gagcgcccca ttggtcccac agcaggccgg ccaggccact ggccagcagc
    11641 agggccagcc cgctcatgag gttgaagaag ccgaacgccg tgccgcgcag gtcggccggc
    11701 gccgcctgcg ccaccatcgc ggccagcagg ccctgcgtca tcgccatgtg cagcccccac
    11761 agccccacac cgccccaaag cagccagccg gcaccgccgg cagccagcaa gaggtcggcc
    11821 gcgatcagca cagccaggcc ccacgccagc agcgttcgat ggctcatcgc gtcggacagc
    11881 ttgccgaagg gataggcgcc aatggcgtac acgaggctca tcgcgatcat caccagcggg
    11941 gtccaggcca tcgccagccc gccttgcagc gcgcgcagca ccagaaaggc ctcggagaag
    12001 cgcgccagcg tgaacacggc gccgacgccg acgacccacc agtaagcacg gccgaggcgc
    12061 cgaagatttt cgcggcggac ggggttgccg cggcgctcgc cggccggccg gggcggttcg
    12121 cgcaccgcga acaccagcaa ggccaccgaa agaaaccccg ggatcaccgc cacccagaac
    12181 acggcccgga agtcgttggc ccacagcagc atcagcccga tcgcgagcga cggtcccaga
    12241 aaagcgccca cggtgtcgag cgcctggcgc aagccgaagg cggcgccgcg gtttgcggcg
    12301 ggcgtgatat cggcgatcag cgcgtcgcgc ggcgcgccgc ggatgccctt gcccacgcgg
    12361 tcgatcagcc gcgccgacag caccaggccc gaggtggtgg cgagcgcgaa cagcggcttg
    12421 gatatcgcgc ccagagcgta gcccagcacg gccagcggct tgcggcggcc ccagtagtcg
    12481 ctgagcacgc cggagaacac cttgacgacc agcgccgtcg cctcggccgc gccttcgatg
    12541 agtcccacca cgagcaccga gatacccaaa gtggtgacca tgaagaccgg cagcaggctg
    12601 tggatcagct cggacgaaac gtccatgagc aggctgacga agcccagggc ccagataccg
    12661 gcgggcaggc gcgaggtcgg ccgggcgcgt gcgttcattc gtgccgcttc aggcgagcac
    12721 gacggctgct cctgcgtccg ctgccgcgat aaccccgatc tcgtacacgg tttcgccgta
    12781 aatgcgcagc gtagcggcca ccgcctgcgc attcgcggcg tctatcacca cgaccatgcc
    12841 gatgccgttg ttgaaagtac ggttcatttc gtggtcggag atcgcggcgg tctgctgcag
    12901 ccaggcgaac agctcggtgc gcggccaact gcccttcttc aggtgcgcgg ccgtgccggc
    12961 gggcagcacg cgcggaatgt tttccagcag tccgccgccg gtgatatgcg cgagcgcctt
    13021 gatcgggtgg gccgccaggg cggccagcac gctcttcacg tacaggcgcg tcggctccat
    13081 gatggcttgg cggaacggct tgccgtccag ggtggcgggc agcttgccgg ccgcgcgttc
    13141 gatgcacttg cgcaccaggg aaaagccgtt ggaatgcgcg ccgctggaag ccaggcccag
    13201 caccacatcg cccggcgtca cgccggcccc ggtgaggatc ttcgatttct cgaccgcgcc
    13261 gaccgcgaaa ccggccaggt cgtattcgcc tggagggtac atgcccggca tttcggcggt
    13321 ttcgccgccg atcagcgcgc agcccgaaag ctcgcagccg gtggcgatgc cgccgactac
    13381 cgccgcggcc gtctcgacat cgagcttgcc gcaggcaaag tagtccagga agaacagggg
    13441 ctcggcgcct tgcaccagca catcgttcac gctcatcgcc accaggtcga tgccgaccgt
    13501 gtcgtgcatg ttccattcga aggccagctt gagcttggtg cccacgccgt cggtgccgct
    13561 gaccagcacc ggctcccggt agcgtttggg gacttcgaac agcgcgccga agccgccgat
    13621 gcccgccaag accccctcgc gcatggtttt tctggccagc ggcttgatgc gttcgaccag
    13681 cgcgtcgccg gcgtcgatgt ccacgccggc gtctttgtag gagagggggg attggctcat
    13741 gggattgggg gaagggaccg cgcagagctc ggcccgatag aatttggcta attttagctt
    13801 cggcacgcat ggccgccatt tgcgttgcgc gccggcgctg gaagcaaggc cttgccggcg
    13861 ctccccccat tcctcatgca attcacgccc acccagaaac atgcagcagc ctggctgctg
    13921 atcgcgctgc tggcggcgct ggcgctgtgg tcgctgggaa gggtactgac gccgttcgtg
    13981 gtggcggccg tgctggccta cgccctgacg cccctggtgg accggctcga cgatctcggc
    14041 cgcggccgca tgccgcgctt tgtcgccgtc gcgatcgtcg agctgctgct catcgtgacg
    14101 ctggttttgc tgatgctgat cgtggtgcct gtcctggcca aggaggcacc cctgatccgc
    14161 gagcaactcc ccttcctctt cgacaggctc aacgccgcgc tggcgccgtg gctggagcaa
    14221 ttgggcatca cgatcgccct cgacctggcc agcctcaagg cgcagctcct gaaatacctg
    14281 agcgccaatt tcgaggacat ggtgggttcg cttttttctt cgctcagggt gggaggcggc
    14341 gtcgcgctga tcgtgatcgg caatgcggtg ctgattcccg tggcgctgtt ctatctgctg
    14401 ctggactgga agctgctcgt gcatcgggtg ctcgaactgg tgcctccccg cgcgcggccg
    14461 ggcgtggact ccttcgccac tgaagccgac caggtgctgg gccagtatct gcgcgggcaa
    14521 ctgctggtca tggtgacgat ggcgattttc tacagcaccg ggctgtcgct gttcggcctg
    14581 gacctggccc tgccgatcgg cgtgttcacc ggcctggcca tgttcgttcc ctacgtgggt
    14641 ttcggcatcg gcctggtgct ggccatcctg gcgggcatcc tgcagttcgc ctcgatcaag
    14701 gcgctggtga tggtggcggt ggtgtatggg accggccagg tgatcgaagg cttctacatc
    14761 acgccgcgcc tggtgggacg ccgcatcggc ctgcacccgc ttctcgtgat tttcgctttg
    14821 ctcgcgttcg cccagctgtt cgggtttgtc ggcgtgctgg tggccttgcc cgccagcgcc
    14881 gttctgctgg tcgcgatccg ccggctgcgg gccggctaca tcggcagcaa cctgtaccaa
    14941 agctgatgaa gcagatcgcc ctcgacatcg gtttggcccg cgccccgacg ctggcgagtt
    15001 tctgcgccgg tcccaacgaa gccgccctca agcacctgca gctgtgggca ggcagcccga
    15061 cccgctcgcc cgtcccgacc tacctctggg gcgcggcggg cagcggcaag acccacctgc
    15121 tcaaggccgt ggcccagcag ctccgcgagc acggagcggc cgcgggctgg ctcgacgcgg
    15181 cgctcctgga gccgcccgat ttcaatgaaa gctgggccgt ggtgctgctg gacgacgtgc
    15241 acctgtacac cgccgtgcag cagcacgcgg ctttcaactg gttcgtcaat gcgcagtccc
    15301 tgcagcgcgg cgtgctcgcc gccggagcgc tgccgccggc cgacctcaag ctgcgcgagg
    15361 acctgcgcac gcggctgggc tggggccatg tcttccagct gcaggtgctg agcgagcccg
    15421 agcggcgcgc cgtgctgcgg caggcggccg acgcgcgcgg cgtgttcctg ggcgacgacg
    15481 tgatggactt catgctcacg cgcttctcgc gcgacctggg cagcctgatg cagctgctcg
    15541 accagctcga tggctatgcg ctgcaaacca aacgcgcgat taccattccg ttgatcagat
    15601 ccatgctgga gaacgagtga gcgtaaccaa gacgcgcctg gcgctgttcg acctcgacca
    15661 caccttgttg ccgatcgatt ccgattacgc gtggggcgtt ttcacgcagg acatcggctg
    15721 gaccgacccg gtggtattca aggagcgaaa cgacgagttc ttcgagcact acaaggccgg
    15781 cacgctggat atccatgact acgtgcgctt cgccaccgag gccgtgcgcc tgcgcggacc
    15841 cgccgaagcc gccgttgccc atgcgcggtt catgcagacg gtgatccggc cggccgtccg
    15901 cgccgaggcg ctggacctgg tgcgccggca ccgggacgcc ggcgagctgc cggtcatcgt
    15961 caccgcgacc aacgagttca tcacccggcc cattgccggc gagttcggcg tggaggaact
    16021 gattgccgtg cagctcgaac gcgggcccga cggctggatc accggcgaga tcgtgggtgt
    16081 gccttcactg cgcgagggca aggtcgagcg gctcgggcag tggcttgccg cgcggggatg
    16141 ggaccggggc gacgtggagg ccaccttcta ttccgactcc atgaacgact tgccgctgct
    16201 tgaagccgtc gatcatccgg tcgccaccaa cccggacgcg cggctgcgtg ccgtcgccgt
    16261 gcagcgcggc tggcgcatac ttgacctgtt caaatgatca aaaaattcat cgacaagctg
    16321 ctcgggacat cgctcggcgg caagggcggc aagcccagct tcggcaagcg ccaggaagtg
    16381 gggccccagg agcatggcat cgatccctcc ctggtggacg atcgggccct gaacgtggtg
    16441 cataccctca aggaagcggg cttcgaggcc tacatcgtcg gtggtgccgt gcgtgacctg
    16501 ctggtgggcc tgcgccccaa ggatttcgac gtcgccacca gcgccacgcc cgagcaggtc
    16561 aagggccagt tccggcgcgc cttcatcatc gggcggcgct tccgcatcgt gcacgtggtg
    16621 ttcggccgcg ggcgcgagca cgaggtgatc gaggtctcca cgttcagggc gtacatggac
    16681 aatgtcgccg ccgaacaggt ggccggcaac gagaaaacca gcaggagcga gctggcgggc
    16741 atgaagcacg ccgtggattc gaccggccgg gtgctgcgcg acaacgtgtg gggccctcag
    16801 gcggaggacg cgacccggcg cgacttcacc atcaacgcca tgtattacga cccggagacg
    16861 cagaccgtgg tggactacca cggcggcatc aaggatgcga agaagcgcac catccgcatg
    16921 atcggcgacc cgctggcgcg ttaccgtgaa gacccggtgc gcatcatccg cgccgtgcgc
    16981 ttcgccgcca agctcaaccc gctgggcttc agtctcgaag ccaagaccgc tgcgcccctg
    17041 gtgcaatgcc aggcgctgct ggccgacgtg ccgcaaagcc gcctgttcga cgagatgctc
    17101 aagctgctgc agaccggcca ttcgctcgcc tgcatcgagc agctcaaggc gctggggatg
    17161 gcgcggggca tctatccgct gctggacctg gtcgtcgagc gtgccgagga acccttcgtg
    17221 aaggccgcct tgcaggacac cgaccggcgg gttggagaaa acaaggcggt ggcgcccagt
    17281 ttcctgctgg cctgcgtgct ctggtccgac gtgcgcgacg gctgggcgca gcggctggcg
    17341 cagaagcacc acccctttcc ggcgctgcag gacgccatcg acgatgtctt caattcgcgc
    17401 atcggggatg tctcgggccg cggcaagctc ggcgccgaca tgcgcgagat ctggatgatg
    17461 cagccgcgtt tcgagaagcg cagcggcaat acgccgttca gcctggtcga gcagccgcgt
    17521 tttcgcgccg gcttcgattt catgcgcttg cgcgcggacg tcggcgaggt cgatgtggtg
    17581 ctggccgact ggtggcagga gttcagccag gccggcgacg cgatgcgcga agatctggtg
    17641 gcgcagttgc gcgaggagca gcacaagggc cagcggcggg tgcgcacgcc gcgtccggcc
    17701 gaaagggccg ccgacaagtc cccgcatgcc ggcggcgcag cgcccgagcc cgtgccggaa
    17761 cgcgaagcgg tcgaggcgcg ggaggccggc ccttccgacg ctccgagaaa gcgccgccgc
    17821 cgccgccgca agccttcggg cgagggcggc gccgggccgg cggccgagcc ggcggaacct
    17881 ctgcagtagg acggccggtg cgggctaccg cggtcatgcg cgatgctgtc cccgcgtacg
    17941 tgggcctggg ggcgaacctg ggagacgccg cgcggacggt gcgcgaggcg atcgcgcgaa
    18001 tcgccgagct gccgcagacc gccgtgacgc gccagtcttc gctgtaccgc acggcgccag
    18061 tcgatgccgc cgggccggac tacatcaacg ccgtggccga gatcttcacg cggctcacgg
    18121 cgcccgagct gctggcgcag ttgaaaggcc tcgagcgggc ggcggggcgc gagcgaccct
    18181 accgcaacgc gccgcgcacg ctggacctgg atctgcttcg cttcggcgac gctcaaatcg
    18241 acagcgcggt cttgcaggtg cctcatccgc gcatgcatgc gcgcgccttc gtcctggtgc
    18301 cgctggccga gatcgcgccg cacctcgttg cgcccgccca gctggcggca gtggcggacc
    18361 agcgcctgtc acgcctttag cccatgaaac tcttcactct ttggttaccc aaatacagtt
    18421 tttccctggc ggtaatgatg gcttttctcg ccatcatctt aacaccgtta aatcaactgg
    18481 gtttgagtca atacaatatc gaaaaaacac tacccgaaaa caaaattgag tttccaggta
    18541 ggaataaaaa cgcgtttaac gtcggggatc gcttaccgat ttatcgtttt aatcccgaat
    18601 gggaaaagcc gattggcgaa gttgaagtgt tggcttatga caacggcaaa tggatcgctg
    18661 gttttaaccc cgatacctta cgttggccca tgggaaatca gggtacgata gtcagagttg
    18721 atgcacaaaa cgtttcgctg aatgtcggac aacgtgtagg tgtaaaagct ggtgatacct
    18781 ggaatatatt cgatggccgc gataacatcg gcaaaattgt aatccgaacg gttcacgagt
    18841 tcgaaagcgt tgcgcagtta gtccagcctt tatcaaataa ttcaactctg gtggggctcc
    18901 aggtaagtcc gtatatcctc gctacccagg ttattgtgtt cgataacctt ttcataagaa
    18961 tattgcagat tacagtgcta ttcacggctt ttacgctctg ggtttggtcg atgttcagcg
    19021 agactccggc taatatctgg ttctctctct gccaagtgtt gagggacaag ataagcgcag
    19081 ctccaactgc agtgcgcttt cgttttttag tgatcattgg tgtgccgatt tgctacggca
    19141 taggtgcatt agtatggaat atactttgtc atataaattg gtatattcac acggcttggc
    19201 ttaatcagtc tatcgagcat tatccagcgt ggggtacgac gtggatgcaa atacttgtcg
    19261 cggctgcatg gtacgcggtt ttatttggca tgctgcgctc gccgatgagt tatctggtcg
    19321 agaggctccg ctataaaccg ctgaactttt cgtggtttcc cttgggtgca aaaccttacc
    19381 tgatttggtt actgcattta tttgtttact atgcctttac caacacgctt ggtcagtacg
    19441 ccgtcgcaaa tatgcaagtc atggtgaacc atgcttggcc agatttccgc gcggcagagg
    19501 agcctgtcat ctcatctgtg tttttgccgt ggatggattt attctctgct aattattcaa
    19561 gtagcaaaca tttagtcgac cgattttatg aattgctggt gcacatgagc acacacgcgc
    19621 cggtgattgc tgaccctgtt atcggatttt tgctgttacg tttgagcatg tggactttga
    19681 caatttgcgt gatcttgtgt ctttatacgt atacggtgat ttgcatactg tggacgcgta
    19741 gtgcggtgcg caatattgat tttacatttg cgggctgggt caccgctgcg gtttgttatg
    19801 gccccttgct aggagatcct ttacatcaga cgattcccag cgcacatggt ttggacccaa
    19861 cctatatcac cgatgtgtgg atgtggtttg ttttggttgt ggaatccctg ctgaatctgc
    19921 tctatatgct atcgatcttt aatttgggat tgatgtttgg catgttagtc gataaaggtg
    19981 tgcgcaccca aggattctac gctgttattc gccatccgaa ctatacgctg gaagcactga
    20041 tgtttgtggt gttgggttta atggcgcttt ctggtccgct gcagtggatt gctatctcgg
    20101 taatttttat aaagtattgg ctgcgttcag agcgcgagga tcaatttatg tcgggttcta
    20161 atcgcgatta tttaacttat aaaacacaaa caccttggaa atttattcca gggttatatt
    20221 aaacgaatgg tacctgctta cgccaagctc gatttcgaaa ccatcttccg ggctcaggta
    20281 gcggatgacg cttttgtgat cggcttccat ttgccgaaag acagacctcc atttgccaat
    20341 ttggaagcag gcgagcattg cgagtgagcc tggcggcaac cttaggttcc ctgccgcatt
    20401 acctcgcgcg taattgccgc gatgacgagg cgggctcaac atggagccat cggaagattc
    20461 atcgacaacg gaggttctca tgagcgacgc cggcctgatt gccatcgcct gcggcttgat
    20521 cgtcggcctg ggggcgatcg gcgcctgcct gggtatcggc accatgggag ggcgctttat
    20581 cgacggcgcg gtgcgccagc cggagctgat ggagccgttg cagacgaaga tgttcctgct
    20641 ggccggcctg atcgatgcca atttcctgat cggcgtcggc gtggcgatga tgttcgtttt
    20701 cgccaacccg ttcgcgaagt gatcagcgct agaaccagtc caccactccc gactgccggg
    20761 cgttctgcgg gttcgagccg gcatcgcgca cgcgctgcaa ggcctcttcg ctgtccagtc
    20821 ccagcgcctt gagcacgcag gccgcggtgc tgccggtgcg gcccaggccc gctgcgcaat
    20881 gcagcaggac ggcatcgccc tggcgcaggg cggcggcaat ctgctgcacc tcgcgccgga
    20941 aggccgccgg gtcttccggc acgccgaaat tgggcatcgg aacgttgagc caccggaagg
    21001 gcaccgtgcc ctgcgccacg gaccggtggt aggcgggcga gagttccgcc aattcgctgc
    21061 gcggcgtcag gcacaccacc agtgccagcc ccgcgcgccg ggcctgggat tcgaaggcgc
    21121 tccaggtttc gaagcgccca ggcatggacc ccagccagag cgagccgggt acctgcgggg
    21181 gcaagacgac gggacgaagc gccatggcgc gattgtgcct tggagcctgc gcggcagaga
    21241 aacgggcctg cgttgggtct gcaagggggt gaaggctaga ccccgcgcgg tcaggctaca
    21301 accggctgcc gccgcgctgc tggctgaccg tgaattcgcc cacggccgtg ccgcgattgc
    21361 cccatgaggt gcggatgtgg gtgatcaccg cggccacctg cgcgtcgctg agcaccgtcg
    21421 cgaagggcgg catgccgaag ggccgggggt ttcccgcggt ggcgggcgga aagccgccgc
    21481 cgagcaccac ctggaccagg ttggccggca ccgccagggt cactgcgcgg ttgccggcca
    21541 gcgccggata ggcacccggc acgccttcgc cgtcgtcacc atggcattgc gcgcaatggt
    21601 cgcgatagag ttttccaccg cgctcggcca tttccccgct gatcatcctc gggccctgcg
    21661 cagcctgggt gtgcggtttg ctctgcggca agctcttgag gtacacagcc atcgcgcgca
    21721 gatcggccgg cgccagatgt tgagtgcttt gcagcaccac ctcggccatg gggcccagca
    21781 ccgtcccgcg cggggacacg ccggcctgca gcagccgcac ggtgtgctcg aggtcccaat
    21841 cccccacgcc ggcctcggcg ggggacgcca gcgagggcgc gtaccagttc tgcatcggaa
    21901 tcaggccgcc ggaaaggtcg atcatgtcgc tgctcgcgcc cagcacattg cgcgtcgtat
    21961 ggcacgcgcc gcaatggccg agaccattga ccaggtacgc gccgcggttc cattccgccg
    22021 attgcccagg gtggtcttca tgcgcgtccg gccggaaata cagagcgcgc cagatggcca
    22081 acgcggtttg tgtgctgtac ggccaacgca agctgtgcgg cgtattggcg cgctcgaccg
    22141 gcgccaggct gcgcaggtaa tcgtagatcg cgtcggcgtc cgcccgggtg accagcgtgt
    22201 agtggggata gggaaaggcc gggtacagca ggcgtccgtc gcgcgagcgg ccgtggtgca
    22261 tggcccgcca gaaatgcgag gccgaccagt tgcccaggcc ggtcgcgtgc ggtgtgaggt
    22321 tgctcgcata cacggcgccg aacggcgttt cgattgcccg gcccccggca aaggcagcac
    22381 cgccgcgctc cgtgtggcag gccctgcaat tgcccacgcg caccaggtag gcgccgcgcg
    22441 ccacgctttc accggccggc ggggtcagcg gcggcgcgtc atggatcttg tcctcgcccc
    22501 agaagttcag gagcagcacc agcgccagca ccgcgagcat gccgaccagc agcgctccca
    22561 gaaaccgcct catggctgct gccttcccgc cggtttcgcc gcgctgccgc aggcgatggc
    22621 cggaggccgg ggcagcgccg ccgcgggccg ggtgctggcg ggcaggggtt gtgccgcgag
    22681 ccagctcgcc acggcggtga gatcctcggg ctcaagatgc cgcgcgatcg ccgccatgca
    22741 atcgggcgcg tgggcctggc gctggccggt cctccaggcg cccagctgcg aattgaggta
    22801 gtcgcgcgaa agcccgagca ggcccggcgt gttgggcagg acgccggtga gcgccgggcc
    22861 atggcattgc gcacaggccg ggatgttctt cgccgcatcg ccgtcccgcg ccagcgtctg
    22921 gcctcgctgc agcgtggcgg gcggcatagt gggtggggct ggcggcgggt acggcaggtc
    22981 cagcgcggcg aaatgctccg caatttcaag caggtaggca tcggacagcg gatccagcag
    23041 gcgggtcatc agcccgtaat gccggcggcc gtcgcggaaa ttgagcagct ggttgtacag
    23101 gtagccggcc ggcttgcccg cgatgcgcgg atagtagccg tcgggcccgg cgcggccttg
    23161 cctgccatgg cacgccgagc acgcgagcgt gcgctgagcc atggtgtcct cgaaaaccgg
    23221 cctggcccgc gcgcaggccg cggcaagaat gacgagcagg aagatcaggg acttcacgat
    23281 atccagacta tgccaccacc ggaccgccgg ccgcgcggca gtcctgttga atcgccggat
    23341 caggggccgg ccggcagccg gaagttgcgc ggcgggctca gtaccaggtg cgattccgcg
    23401 tgcgcgatgc acagcaggat ccagccctcg gccttttcct cggccagcag gcctggccat
    23461 tgaatgcggt agcggacccg cccctgaagg acgcggcaca tgcaggtgcg gcaagtgccg
    23521 ttgcggcagg aactgggcag ctgcaagccg gcttgctcgg ccgccagcag cagcggctgg
    23581 ccggcgctcg cggtaaagcg cggtccgccg ggctcgaagc ggacgtcaaa gcgcgccgca
    23641 ggcgcatcgc tggaaaacaa gggcgccggg ccgctgtcgt cggtcatggc ccatcataat
    23701 cagcgcctta gggcctgttc aggccccaga cccgcatttg acctatgtcc agctacccgt
    23761 tgcgtcctgc ggtggccggc gatgccgaac aggtggccgc cgttcacgcg agttcttccc
    23821 acgccgctta tgaagccgtc gcacccgagg cgcaccagag cctgccgatc gagcggcgcc
    23881 gcgctttctg gcgcgaggcc atcgagttcg gcgagcccct ggtccaggtg gcgctcgagg
    23941 ccggccgggt gatcggcttc gccggttacg accgctcgcg cgatcccaag tccaaagcga
    24001 cgacgggcga gatctgggcg atgtacgtgc agccctcgca ctggggccgc ggtgtgggac
    24061 tggcattgtg ggacgccacc cgggaggccc tggtggagga gggctgcacc gaggtcacgt
    24121 tgtgggtgcc gctgtgcaac gaccgcgcgc tgcgtttctt cgaactcgcc ggcttcaagc
    24181 gcgaactcaa caccgcgaag accgccgtcc tcggcggcgt caagctcgaa gaaattcggc
    24241 tgcggcgcgg gctcgactga cggcgcgcga ggacccatca tggcatcgag cctgctcgcg
    24301 ctgctggacg acatcgccac gatcctggac gacgtgtccg tgctcgccaa ggtggccgcc
    24361 aagaaaaccg cgggggtgct cggcgacgac ctggccctga acgcgcagca ggtcaccggg
    24421 ctggcggccg agcgcgagct gcccgtcgtg tgggcggtcg ccaagggctc gctcgtgaac
    24481 aaggcgatcc tggtgcccgc ggcgctggcg atcagcgccg tcgccccctg ggccgtcacg
    24541 ccgctgctca tggtgggcgg cgctttcctt tgctacgagg gcttcgagaa gctggcccac
    24601 aggttcctgc acagccgctc ctacgatgaa gcccaccgcc aggaactgga gcacgccctg
    24661 ctcaatcccg acatcgactt gcgggtcgtc gagaaggaca agatcaaggg ggccgtgcgt
    24721 accgatttca tcctctcggc cgagatcatc gcgatcacgc tgggcacggt cgcggacagc
    24781 gcgttcgcgg tccaggtcgg ggtgctcggc ggcatcgcca tcctgatgac catcggcgtc
    24841 tacggcctgg tcgccggcat cgtcaagctc gatgacgccg gcctttacct cagccgcaag
    24901 agccaggcga ttgcgcggtc ggcaggcaca gccatcctga gcgccgcccc gtggctgatg
    24961 aagggcttgt cggtcgcggg cacggcggcg atgttcctcg tgggcggtgg catcctggtg
    25021 cacggcgtgg cgccgctgca ccacgccatc gaacgtttcg ccgaaagtgc cggcgccggt
    25081 ttcggcgcgt tgctgccgat gctgctcaac gccctggtcg gtgtcgtcgc cggcgctgtg
    25141 gtgctgggcg tggttacgtt ggcgcaacgc ctggctcgcc gcagggccgc tgtgtgagcg
    25201 gcccgccgca actgcactgg acggaaggca gcggcgagcg cagcgccctc tggcgctcgg
    25261 aggcggcagt accgcctccc aagcgggtgc aagtggtgga cgataccttg tcagccgacg
    25321 ccgcctaccg cctcgccagc gaaggcacgg ccctgctgtg gcgcagcgac ttccataacg
    25381 cgcggcaact gctgcaggcg atggcccgcc gcgccgaccg caagccccgc aatccggccg
    25441 ccgcgcccgc gtcgcccacc gaggcgttcc acctgcaccg ccaggcccaa ggccagcgct
    25501 cacgcatact gggccgcttg ctgatcgcac tggaaggcga ttaccgcatc gccctgccgc
    25561 gcgcgcccga cctgcgccag gcttgcctcg aggcctgggg cccggccgac ggccagccct
    25621 ctgtggcttc gctgcgcgag ctgctggggc tggtcggggc gcacgaatgg cgcaagaagg
    25681 gggtcgaggt tccggcgctc ggtgcgccgc ccggcaaccg catccatccc cattacggcg
    25741 tgttttcccc ggtgcgcggc gaatacctcg aactggtggc caaggcgcct ttgccctcga
    25801 aggacctggc cttcgacatc ggcaccggca ccggcgttct cgcggcgatc ctggcccgcc
    25861 ggggtgtgcg gcgggtggtg gcaacggacc aggattcgcg cgcgctggca tgcgcccgcg
    25921 aaaacgtggc gcggctgggt ctgcaggaca aggtggaagt gcggcaggcc gacctttttc
    25981 cgccggggac cgcgccgctg gtgatttgca atccgccctg gttgccggca cttccgagct
    26041 caccgctgga gcgcgcgatc tatgacgaag acagccgcat gctgcgcggt tttctcgccg
    26101 gcctggcggc ccacctcgcg ccgggcggcg aaggctggct catcctgtcc gatctcgccg
    26161 agcacctggg cctgcgcagc cgcgaggact tgatggggct gatcgagacg tccggcctgc
    26221 gggtggtcgc tcgcagcgac gtgcggccgg gccaccccaa gaccggcgat acccgtgacg
    26281 ccctgcatgc ggcgcgcgcc gcggaagtca cttccttgtg gcggctggtg ccgttgcaga
    26341 ccggccaggc tcggctgtag gcaagctcct acgccattag gcgagctcgg cggatacgaa
    26401 gccggtggct ctgcggtaat gatggacgca tccccaacga accggagcgt gtcatgcccg
    26461 agccgtccca acaaccgctt ccccccgctc atccgcgccc ggccgccgcg ctggtggaga
    26521 cgaccgcacg ctggctcgac atcgacgacg acctcgcgct gggatgcgaa agcgccgacc
    26581 cctcactgca gatcgagcgc tggggccagg aggttcttcc cttccccgca acctcatcct
    26641 gacggtgagg acgcgtcgca tatcgtgaca gcgccggtcc tacaggcgct gccgtcatat
    26701 ccccactttc caggttgggg cccgggccta aggtgagaaa cagctggcag cgtgccggca
    26761 tttttcttcc cagtggcggc ggcaagttcg tccgtcgtgg gagtcccttt caaaggagtt
    26821 accgatatga atgcaaccaa gaaaagtctg accaccttgg ccgtggcggc cgccctggcc
    26881 ggcggcgtgg gcctggcgat tgcccagagc acgagcaccg aaacgccggc ggccgcgggc
    26941 agctcgtacg gcgccacgca gagcagcgac acgcaaagca gcggggctgg tcccagcaca
    27001 gcgggcaccg cgactctggg cggcagcacg gacagcagct cttccacgac gatgtccaac
    27061 accgacagca cgaccgagga gcgcgttgcg cagatcgacc ggaactaagc gccttaaggc
    27121 cccatcgaag ccgctgacgg cttacgatgg ggtttctttt ttggagaggg agtgtcagtc
    27181 cgaccgacaa tgaatccgtt gcccacgccc agatccgcca gttccgaatt cctggattcg
    27241 gtccagaaag gactgttgct gggcgcggcc gtgctcgcgc tggtgctgcc ccccaatatc
    27301 gggatcgtgc accggcaggc gtatgcgccc gcaacgcatg cgcccgcgcg catggcgccg
    27361 ccgccggtcc ggctcgccga tttcgcgggg agggtcccga gcccggatgt gcgccacctg
    27421 gccaactggg ccgtgcaaac gcgcgaccac cagaacaggt ccctcgtcat tgtcgacaag
    27481 aaaggcgcca gcatttacgt cctcgacccg gccggtcgcc tgcaagggaa cgcgccggtg
    27541 ctgctgggtt cggccgtggg cgaccatacc gtgcccggca tcggcgacaa gccgatcgac
    27601 caagtgcttc ccgaggagcg cacgaccccg gccggacgct tcgttgccga agtcggcatg
    27661 aacatgcgcg gcgaggacgt cgtctgggtc gattacgact ccgccgtgtc gttgcatcgc
    27721 gtgctcacca ccaacccctc ggagcggcgg ctagaacggc tggcctcgcc cgaggtggag
    27781 gacaaccgga tttcgtacgg ctgcatcaac ctgccgcgcg acttcttcga aaccgtactc
    27841 agccccgccg tgcgtacggc cggtgccatc atctacgtcc ttcccgagac acgctccgtg
    27901 caacaggttt tcggctccta cgacgtcgag gccgcgacga atcttgcgcg gcgttgaacc
    27961 tgcttgcaaa gcccctacaa gctgtcagtt aacgttacaa ggcttgtgtc cgggtaaccg
    28021 gagttggtag cattgcccct gctgagccgg gccggtgccc ggcggccgcg ctcgaagcgc
    28081 gaatgcatga ggggtcggga tgggtcgcag gggacgggct tgggcggctg ccgcgttgtg
    28141 gggaatcgtt ggggcgacgc cagcgttggc ggaacaaata gcggcgccgg atttcggcac
    28201 gcacctcgcg tcggcggatg cgcgcgacgt cgcgcggttg atattcgagt cggccgaccc
    28261 ggccggcctg ccgttcgcca tcgtcgacaa gaaggaagcg caaatctatg tcttcgatgc
    28321 gcagggtcag ctgtcgggcg tttcgcccgc gctgctgggg caagcccgcg gcgaccattc
    28381 ggctccggac gtcgggcgga aagcgcaaac gggcagcgtt ccccttaacg aacgaacgac
    28441 gccttccggc cgtttcatat cccggcccgg acgcaacctc gacggcgaac acgtcgtgtg
    28501 ggtcgattac gatgccgcct ttgcaattca ccgtcttcgg ccgggtgcca gccagccaca
    28561 gcgcgtggcg cggctggcct cggcgacgcc tcacgacaac cgggcgtcgc tgggttgtgt
    28621 ggtggtcccg gaaactttct acagcgatgt ggtgcaaccg ctgctggggc gtacccgcgc
    28681 ggtgatctac gtgctgcccg aagagcgtcc cgcgcgcgaa ctgttcaacg cgatctgagc
    28741 atgtcccgct caggttgtcg cggtgctgag cctgcgcgct cggggcaggc atcgtcttac
    28801 accgattcat ggtgagtgct gacgtatccg tttcggggag ctcatagcct tggtgcagct
    28861 ggcgatgagg ccggcgattt tcaccaccat tcaaggagtc tccgaaatga ccgcatcgac
    28921 cccgttcaga ggtgtgctcg ccgccctggc agcgtccaag gagcggacgg tgcgcgcaga
    28981 tcgcaactga tccaaggctg tcacactaaa aaaaac
//
LOCUS       DASWIY010000006         7073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1480191, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000006 DASWIY010000000
VERSION     DASWIY010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480191"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VGA59_00330"
     CDS             complement(<1..1117)
                     /locus_tag="VGA59_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding peptidase"
                     /protein_id="HEX9718149.1"
                     /translation="MKTFRCDHCGHALFFENVQCLQCGSDLAFLPDRLALCAVEPVPG
                     GQAGLWRRKTRHRPKGEARNYRLCHNHTEYQACNFAVQVADPNVLCVSCRQTRILPDL
                     SVPDNRERWYRIEAAKRRLFYTLAKLGLVAVFPPNGQRDGPIFEFLADVPGQQVLTGH
                     CEGVITLNIAEADDTERVQRRVALHEPYRTLLGHLRHESGHYYWDRLVRDNAHVDAFR
                     QMFGDESAGYNEALDAYYAAGGAPADWQARHVSAYATSHPWEDWAETWAHYLHMVDLL
                     ETAASFSTRLAVPGSDDEDVEEVANPFETGAGDFDQLVDQWIPLTLLLNSLNRSLGQD
                     DAYPFALTPGALQKLRFVHDLIQSHQRRSAPAAATSAC"
     gene            complement(1212..1526)
                     /locus_tag="VGA59_00335"
     CDS             complement(1212..1526)
                     /locus_tag="VGA59_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX9718150.1"
                     /translation="MKYARALAFAVLAGATIVTTGCAVIRGQETPGAYVDDVTITTAV
                     KAKFVEDKIVDAGAIKVQTLNGTVQLSGFAKSNAEKAKAEFIARNTKGVRQVQNSLLV
                     RP"
     gene            complement(1613..2068)
                     /locus_tag="VGA59_00340"
     CDS             complement(1613..2068)
                     /locus_tag="VGA59_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718151.1"
                     /translation="MSDAADRLARSRLAILDEIQPVERRPRRRGQEPRREREADGGQP
                     HDDAGRGGALGWFGRFRVAASTWWRHHPAQLGLELATPVLSAYAGRKPLQFLGIAAAV
                     GAVVVVTRPWRLISVTGLLLAIAKSSQLSTVLLSALSAADYQRDHEPRD"
     gene            complement(2136..2489)
                     /locus_tag="VGA59_00345"
     CDS             complement(2136..2489)
                     /locus_tag="VGA59_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718152.1"
                     /translation="MLIKRPELVIDHVAGYAALVQEEASTVGVQVAKRVIAWGVAVLG
                     LVVFLVLAGVAVMLGAMQGEFHWVLVAAPGAALLLSIGAWSVARQQLPAKAFTELKAQ
                     LDADAQALRSVGAQT"
     gene            complement(2530..2892)
                     /locus_tag="VGA59_00350"
     CDS             complement(2530..2892)
                     /locus_tag="VGA59_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718153.1"
                     /translation="MMTPTSKSLRAGASGYSDEALGAGAQAFESTKHLASQAIDKAGE
                     TVRDLRYGATDLARRGASTVSEATAAAQRQLGHYAQATGRYVSEEPVKSALIAAAIGA
                     AVAALVLAIRRNRRDDYY"
     gene            complement(2957..3121)
                     /locus_tag="VGA59_00355"
     CDS             complement(2957..3121)
                     /locus_tag="VGA59_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX9718154.1"
                     /translation="MLHYAIVFLVIALIAALFGFGGIAAGAVEIAKILFFVFVIMAVV
                     AFVISLGRKG"
     gene            complement(3314..4669)
                     /locus_tag="VGA59_00360"
     CDS             complement(3314..4669)
                     /locus_tag="VGA59_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX9718155.1"
                     /translation="MRWFPMPKKAVSLALALLAACVLIVINETGFDQSTDALDNIAEA
                     SHSRASLNRVLQHVLDAETGSRGYLLTGDPRYLEPYDAAVAAIGRQLDALRLIYTPSA
                     DDFTILAQLTRNVQRKLAEMDLSVRMRKQGNEDAWKFVLMTDVGKEHMDAIREEATKL
                     INSSAARMEISQVKVRRSLLLSRIGIATVTLAGLLAFYLYLRQTSALELAGERQQQML
                     QQERDLLERQVRERTATLAELATHLQQVREEERGHLARELHDELGALLTAAKLDVARL
                     KSRLGAQPSPEIAQRLQHLTEALNSGIALKRRIIEDLRPSSLANLGLTASLEILAREF
                     SERSGVEVMTSVESTELDESRQLTVYRLIQESLTNVGKYAEAKQVDISLRNYGNHVEV
                     DIKDDGKGFNVAEIRPLTHGLAGMRHRVEAAGGRLTVISAPAKGTRISAVLPKGSPVA
                     A"
     gene            4794..5426
                     /locus_tag="VGA59_00365"
     CDS             4794..5426
                     /locus_tag="VGA59_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9718156.1"
                     /translation="MIKVGIVDDHAIVRSGLRQFFSEQVDLRVVGEAASGREAIDLVR
                     TTELDVLVMDLSMPGQSGIDALGMIRAKAPDVGILILSGYPEEHYAMNLIRQGASGYL
                     NKECEPMEIVNAIRTIALGRRYISPAVAELLAQQLNRKEGGAPHEQLSEREFQVFLKL
                     AKGETAGDIAKALSLSVKTVSTYRTRLMEKMSLASNSDLTYYALKNKLID"
     gene            complement(5442..5831)
                     /locus_tag="VGA59_00370"
     CDS             complement(5442..5831)
                     /locus_tag="VGA59_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9718157.1"
                     /translation="MELRTYIIEDNATIRENLIATLEELSSVKALGWAETENEAKSWL
                     DRHGDEWDLAIVDLFLKQGSGLGVLEACQGRLGAKRVVVLSNYATADMRKRCAQLGAD
                     AVFDKSNEIDALIEYCIELCEAPQPDA"
     gene            6098..6292
                     /locus_tag="VGA59_00375"
     CDS             6098..6292
                     /locus_tag="VGA59_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718158.1"
                     /translation="MRLLIESAIIFFGTLMAAALVALSGPAIVDLVTGQATPSQAATQ
                     VLASSQPRDVEATSAPRPGR"
     gene            6302..>7073
                     /locus_tag="VGA59_00380"
     CDS             6302..>7073
                     /locus_tag="VGA59_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX9718159.1"
                     /translation="MDGTAATAGPRSRALWVKVLAGFMVLAAVLALLVAFFPWDLLRG
                     PLNRYVSEKTGRHFEITRKLDVKLGRTARIFADGLEFANPDWAQDPHLVKADGAEIRI
                     ELLPLLTRHIELPLVVLNKPRLSLQVEADGRRSWALGRDTSDPRNIPDIGALIVDQGS
                     MHYIATHHGADIHTDFAIQGPLKPPAATGSTTTTVATSTTAAAAASAASAASAATGAA
                     LPLSFKSKGTWKKEPFTAQGRTGNVLYLSAPLKHPFPME"
BASE COUNT         1175 a         2329 c         2318 g         1251 t
ORIGIN      
        1 cgcacgcgga cgtggcggcg gcaggcgcac tccggcgctg gtgcgactgg atcaggtcat
       61 gcacgaaacg cagtttctgc aaggccccgg gcgtcagggc gaaaggatac gcatcgtcct
      121 gtcccaggct gcggttcagg ctgttgagca acagcgtcag cgggatccac tgatccacca
      181 gctggtcgaa atcgccggcg ccggtttcga acgggttggc gacctcctcg acgtcctcgt
      241 cgtcgcttcc gggcacggcc aggcgtgtgc tgaacgacgc agcggtttcc agcaggtcca
      301 ccatgtgcag gtaatgggcc catgtttcgg cccagtcttc ccagggatgc gaggtcgcat
      361 aggcgctgac gtgccgggcc tgccagtcgg ccggcgcgcc gccggcggcg tagtacgcgt
      421 ccagcgcctc gttgtagccg gcgctctcat cgccgaacat ttgccggaaa gcgtccacgt
      481 gagcgttgtc ccgcaccagc cggtcccagt agtagtggcc cgactcgtgc ctcaggtggc
      541 ccagcagcgt gcggtaaggc tcatgcaggg cgacgcggcg ctgcacccgt tcggtatcgt
      601 cggcttcggc gatgttgagc gtgatcacgc cctcgcagtg gccggtcagc acctgctggc
      661 ccggcacgtc ggccaggaac tcgaagatcg gcccgtcgcg ctgtccgttc ggcggaaaca
      721 ccgcgaccag gccgagcttg gccagggtgt agaacagccg gcgcttggcc gcctcgatgc
      781 ggtaccagcg ctcgcggttg tccggcaccg acaggtccgg caggatgcgt gtctggcggc
      841 aggagacgca cagcacattc ggatcggcca cctgcaccgc gaagttgcag gcctggtatt
      901 cggtgtggtt gtggcacagc cggtagttcc gggcttcgcc tttggggcgg tgccgcgtct
      961 tgcggcgcca aagccccgcc tggccgccgg gaacaggctc caccgcgcac aatgccagcc
     1021 gatccggcag gaaggcgagg tcgctgccgc attgcaggca ttgcacgttc tcgaagaaca
     1081 gggcatggcc gcagtggtcg cagcggaagg tcttcatcgg gtcttggtct tccaaagaaa
     1141 aaaggcaggc ttggcagggc ctgccttgtt tgcaagccgg ctcgccgcgc cggcctttcc
     1201 cgccccgtgg cttacggccg cacgagcagg gagttttgca cctggcgcac acccttggtg
     1261 ttacgcgcta tgaattcggc cttggccttt tcggcgttgg acttggcgaa gcccgacagc
     1321 tgcacggtcc cattgagcgt ctgcaccttg atggcgcctg cgtccacgat cttgtcttcg
     1381 acgaatttcg ccttgaccgc ggtggtgatc gtgacgtcgt cgacataggc tcccggggtt
     1441 tcctggccac ggatcacggc gcagccggtg gtgacgatgg tggcacccgc caggacggcg
     1501 aatgcgaggg ctcgggcgta tttcatgact ttctccttga gttggttgga ttctcggtgc
     1561 cgaagcttcc caaaagctcc ccggatggcc gcaccgcacc atccggcttg cgctaatcgc
     1621 gcggttcgtg atccctctgg tagtccgccg ccgaaagcgc ggacagcaag acagtggaca
     1681 gctgcgacga cttcgcgatg gccagcagca ggcccgtgac cgaaatcagg cgccacgggc
     1741 gggtgaccac cacgaccgcg ccgaccgcgg ctgcgattcc cagaaattgc agcggcttcc
     1801 tgcccgcgta cgccgacagc acgggggtgg ccagttccag ccccagctgc gcaggatggt
     1861 ggcgccacca ggtcgaggcg gcgaccctga aacggccgaa ccatccgagc gcgccgcccc
     1921 ggcccgcgtc gtcgtgcggc tggccgccgt ccgcctctcg ttcccgccgc ggttcctgcc
     1981 cgcgacgccg cggccgccgc tcgacaggct ggatctcgtc caggatggcc aatcggctcc
     2041 gggccagtct gtcggcggca tcgctcatgc cgcggcctcc gccgggtgat ggttcggatg
     2101 cacactcggg ggtcggccgc ctgcgccctt cgcgctcatg tctgggcccc gaccgagcgc
     2161 aacgcctggg cgtcggcatc gagctgggct ttcagttcgg tgaacgcctt ggctggcagc
     2221 tgctgccgcg ccacgctcca ggctccaatg gacagcagca gggcggcgcc cggcgccgcg
     2281 acgaggaccc agtggaactc gccctgcatg gcaccgagca tgaccgccac ccctgccagg
     2341 accaggaaga cgaccaggcc gagcacggcc acgccccagg cgatgacccg cttcgcaacc
     2401 tgtacgccca ctgtggatgc ttcttcctgc accagcgccg catagcccgc gacatggtca
     2461 atgaccagtt ccgggcgctt gatcagcact gcaaatatgg gatgtaccat gccgtgcctt
     2521 ggcgcgggcc tagtagtagt cgtcgcgccg gttgcggcgg atcgcgagca ccagagcggc
     2581 cacggcggcc ccgatcgccg ctgcgatcag cgccgacttc accggctcct cggatacata
     2641 ccgtccggtc gcctgggcat agtggcccag ctggcgctgc gccgcggcgg tcgcttcgct
     2701 cacggtgctg gcgcccctgc gcgccaggtc ggtggcgcca tagcgcaggt cccgcaccgt
     2761 ctcgcccgct ttgtcgatgg cctggctggc caggtgcttg gtgctctcga acgcttgggc
     2821 gccggcgccg agcgcctcgt cggaataacc ggaagctcct gcgcggagcg atttgctggt
     2881 gggggtcatc atgtgaatcc tctcttggtg gttggcgcgg ggccggcggc cccgcgaaaa
     2941 aagccgtggc ggaacgctaa cccttgcgcc cgaggctgat gacgaatgcg acgacggcca
     3001 tgatcacgaa gacgaagaaa aggatcttcg cgatctcgac cgccccggcg gcgataccgc
     3061 cgaagccgaa cagggccgcg atcagcgcga tcaccaggaa cacaatggcg taatgcaaca
     3121 tgtcaatctc cttcgcggga cgcgcccggt tcaggcgcct cgcaagccgc gcaaaacgcc
     3181 tgcagcaagg ttaggccgcc acgtcagctg taggtgatca gcggcaggcg tgagtgtttg
     3241 tcggtagggg ggatggctga gtgtaggact gcgccgacgg ccgggaagga ggtttcggac
     3301 accggtccgg gattcatgcc gccaccgggc tgcccttggg caggaccgcg gaaatgcgcg
     3361 ttcccttggc cggcgcggag atcaccgtga ggcgtccgcc cgcggcttca acccggtgcc
     3421 gcatgcccgc caggccgtgg gtcaaggggc ggatctcggc gacgttgaaa cccttgccgt
     3481 cgtccttgat gtccacttcc acgtggttgc cgtaattgcg caggctgatg tccacctgct
     3541 tggcttccgc gtacttgccc acgttggtga gcgactcctg aatcagccgg tagacggtga
     3601 gctggcgcga ttcgtccaac tcggttgact ccacgctggt catcacctcg acgccggagc
     3661 gctcggaaaa ttcgcgcgcc aggatctcca gcgacgccgt caggcccaga ttggcgagcg
     3721 acgaaggccg caggtcctcg atgatgcggc gcttgagggc gatcccgctg ttcagggcct
     3781 cggtcaggtg ctgcaggcgc tgggcgattt cgggcgaggg ctgcgctccg agacgggatt
     3841 tcaggcgcgc gacgtccagc ttggccgcgg taagcaaggc gcccagctca tcgtgcagct
     3901 cgcgcgccag gtgacctcgt tcttcctcgc gcacctgctg caggtgagtg gcgagttcgg
     3961 ccagggtggc ggtgcgctcg cgcacctggc gctccagcag gtcacgctcc tgctgcagca
     4021 tctgctgctg ccgttccccg gcaagctcga gcgcgctggt ctggcgcagg taaaggtaga
     4081 aggcgagcag gccggccagg gtgacggtgg cgatgccgat gcgcgacagc agcaaggatc
     4141 ggcgcacctt gacctggctg atctccatcc gtgccgcgga gctgttgatg agcttcgtcg
     4201 cctcctcgcg gatggcgtcc atgtgctcct tgccgacgtc cgtcatcagg acgaacttcc
     4261 aggcatcctc gttgccctgc ttgcgcatgc gcaccgacag gtccatctcg gccagtttgc
     4321 gctggacgtt gcgggtgagc tgggccagga tggtgaaatc gtccgcgctg ggcgtgtaaa
     4381 tcagccgcag cgcgtccagt tgccggccga tggccgccac ggccgcgtcg tagggctcca
     4441 ggtagcgcgg gtcgccggtc agcaggtagc ctcgggagcc ggtttcggcg tccagcacat
     4501 gctgcagcac acggttgagg gaggctcggc tgtgggaagc ttcggcgatg ttgtcgaggg
     4561 catcggtcga ctggtcaaag cccgtttcgt tgataacgat caagacacag gcggccaaga
     4621 gggcaagcgc caggctgacc gccttcttgg gcattgggaa ccaacgcatg aatttcactc
     4681 caaaatgacc gggttgtgca taaaattcca gctaatcatg gctgaagggt aggacttttc
     4741 agcggaaagt tgggcacaat gggcttcaaa cttgatatcg aaagcgaaca gacatgatca
     4801 aagtcggcat tgtggatgac cacgcgatcg tgcggtcggg gctcaggcag ttcttttccg
     4861 aacaggttga tctgcgcgtg gtcggagaag cggccagtgg ccgcgaagcg atcgacctgg
     4921 tgcgcaccac cgaactcgac gttctcgtca tggatctgtc catgcccggg caaagcggca
     4981 tcgacgcgct gggcatgatc cgtgccaagg cgccggatgt cggcatcctc attctctcgg
     5041 gctatccgga agagcactac gcgatgaacc tgatccggca gggcgccagc ggctacctca
     5101 acaaggaatg cgagccgatg gagatcgtca acgcgatccg caccatcgcc ctgggccgcc
     5161 gctacatctc gcctgccgtc gccgaactgc tcgcccagca gctcaatcgc aaggagggag
     5221 gagcgcctca cgagcagctg tccgagcgcg agttccaggt gttcctcaaa cttgcgaaag
     5281 gcgaaactgc cggcgacatc gccaaggcgc tgtcgctgag cgtgaaaacc gtcagcacct
     5341 accgcacccg gctgatggag aaaatgagcc tggcatcgaa cagcgacctc acctattacg
     5401 cgttgaagaa caaactgatc gactgactgg cgcgggcgcg ttcaggcatc gggctgcggc
     5461 gcctcgcaca gctcgataca gtactcgatc agcgcatcga tctcgttcga tttgtcgaac
     5521 accgcgtcgg ctcccagctg cgcgcagcgt tttcgcatgt ccgccgtggc gtaattgctc
     5581 agcacaacca cccgcttggc gcccaagcgc ccctggcagg cttccagcac ccccagtcca
     5641 ctgccttgct tcaggaacag gtcgacgatg gccaggtccc attcgtcgcc gtgccggtcc
     5701 agccagcttt tcgcctcgtt ctcggtttcg gcccagccca gggccttgac cgacgacagt
     5761 tcttcgagtg tcgcgatcag gttttcccgg atcgtggcgt tgtcttcgat gatgtaggtt
     5821 ctcaattcca ccgtgagtcg attcaaggcg ggagcaggcg ccccgctgtt ttttttcagc
     5881 cccggccggg ctcgttgacg tgtatcggca tgtgtcccct accgccactc attctgccag
     5941 ccaagcgagt gccgcttcgt aggaagtttc ctacgcccag catgtaggca accgctgacc
     6001 gcagctcccg caagccactg accgcgcaac actttttcac ggcgcacact aggctgaaac
     6061 acagccggtg cgcgccggct tgtcaaggga tggcgcaatg agactgttga tcgaatcggc
     6121 cattattttt ttcggcaccc tcatggcggc ggcgctggtg gctctgagcg gccccgcgat
     6181 cgtggacctc gtgacagggc aagcgacgcc gtcgcaggca gcaacgcaag tgctggcttc
     6241 cagccagccg cgtgacgtcg aagctaccag tgcgccacgg ccgggccgtt gaggcgaccg
     6301 gatggacggc acggcagcga ccgccgggcc ccgcagccgc gcgctgtggg tgaaggtgct
     6361 tgccggtttc atggtccttg ccgccgtgct ggcgctgctg gtcgcgtttt tcccctggga
     6421 cttgttgcgc gggccgttga accgctacgt cagcgaaaag acggggcgcc atttcgagat
     6481 cacccgaaag ctggacgtca aactgggtcg caccgcccgc atcttcgccg acgggctcga
     6541 attcgcgaat cccgactggg cccaggaccc gcatctggtc aaggcggacg gggccgagat
     6601 ccgcatcgag ctgctgccgc tgctcacgcg ccacatcgag ctgccgctgg tcgtgttgaa
     6661 caagccccgg ctttccctgc aggtggaagc cgacggccgg cgcagttggg ccctcgggcg
     6721 cgatacctcc gacccgcgca atattcccga catcggcgcg ctgatcgtgg accagggcag
     6781 catgcattac atcgcgaccc atcacggcgc cgatatccat accgacttcg cgatccaggg
     6841 accgttgaag ccgccggccg cgaccggctc gaccaccacc acggtcgcaa ccagcaccac
     6901 cgccgcggcg gctgcatccg ctgcatccgc agcgtccgct gcgacggggg cggcgctgcc
     6961 cctgagtttc aagtccaagg gcacctggaa gaaggagccg ttcacggccc agggccgcac
     7021 cggcaacgtg ctttacctga gcgcgccgct gaagcaccca ttcccgatgg aag
//
LOCUS       DASWIY010000007         4013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2219575, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000007 DASWIY010000000
VERSION     DASWIY010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219575"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VGA59_00385"
     CDS             complement(<1..880)
                     /locus_tag="VGA59_00385"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9718160.1"
                     /translation="MQHLSESATQGSRPLPLAGVRVLDISQVMAGPYACMLLADLGAD
                     VIKIEPPGGGDQTRGSMGFKMKGPDSMGFLNMNRNKRSVALNLKSEGGRAVLLRLVKD
                     ADILVENYRPGAMKRLGLGYDDVKALNPRLVYTSISGFGQTGPWADRPGFDLMAQAMS
                     GVMSVTGYPGGPPVKAGVPVADIGCALFAIYATLAAYIGAKATGQGQYIDAALFDSAL
                     AFSVWDICDYWGTGKPPEPLGTANKMTAPYQAMAASDGYFVMGANNQKLWEKLCTLMD
                     RRELLQDERFSTISLRL"
     gene            complement(891..2177)
                     /locus_tag="VGA59_00390"
     CDS             complement(891..2177)
                     /locus_tag="VGA59_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9718161.1"
                     /translation="MAIMFTLFGSVLVLVLANVPIAVSLGIVAVIAMVASHGLGSLPN
                     MPLVIYNGATNFPLLAIPLFILAGAIMNASGISQRLIAFASALFGWVRGGLSHVSIGT
                     SLFFAEISGSAVADVAALGSILIPGMKAKGYPSAFAAASMSSAATLAVIIPPSIPMIL
                     YAVMAESSVVQLFVAGIVPGLIGGIGLMGMATFLARRYNLPREDRFSWARVRRTGREA
                     LWAFALPIIILGGIFGGIVTATEGAALAVVAALFVGLVVYRELDLKHLRAAVMEGGVQ
                     TAVVMLLVATSALLGTYLTEVQAPQALARAVSDFTSNKWVVLGLLNVLFLFLGMFLHS
                     AAAIILVVPIVMPLVRAVGIDPVHFGLIVTINLGIGQQTPPVASVLMVASSIAKCSVW
                     EVSKVNVWFIGVLVAVLMLVTYVPVTGLGLVQLFYR"
     gene            complement(2202..2750)
                     /locus_tag="VGA59_00395"
     CDS             complement(2202..2750)
                     /locus_tag="VGA59_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9718162.1"
                     /translation="MHDTMAPHAGSAMHRFRGRYGLFLEWVVMVLMVALAVEVTLGVL
                     FRAGGSSLVWYDEVASVLLAWLTFYGSALASVKRAHIACPEIVDQMPPGFRKAVNIFG
                     QVLVILFFTLLGWMGVAIMPALVGEALVSLPWIPMNVVQSVIPVSAVLILVGEVMHLI
                     DLAGSRHHAAAGSGAALADGLH"
     gene            complement(2761..3759)
                     /locus_tag="VGA59_00400"
     CDS             complement(2761..3759)
                     /locus_tag="VGA59_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9718163.1"
                     /translation="MIADSLKRIAALLLVVAGAAFALHGTAVAQPVELKLGHVGEPGS
                     LFQMSADEYAKRVNEKLAGKVKVVTYGSSQLGGDKEMIQKLKLGTIEMALPSTVMTSE
                     VDLFGIFELPYIVKDRAHMARIEKDVFWPTLAPETEKKGLKVLAVWENGYRHITNNKK
                     PVKVPEDLKGFKLRVPEGKWRVKMFQEYGANPSPMKFSELFTALQTGVMDGQENPFTQ
                     IYSAKLQEVQKYLSLSGHVYTPAYLLVGSRKYATLPADVRKVLEDTARETQAFVYATA
                     AKQETELLEKLKQAGMQVNEVDKNAFIAASKGIYDEFGKEVAGAKPLIDKAVALGK"
BASE COUNT          755 a         1352 c         1241 g          665 t
ORIGIN      
        1 cgagccgcag ggagatggtc gaaaagcgct cgtcctggag cagttcacgc cggtccatca
       61 acgtgcacag cttttcccac agcttctggt tgttcgcgcc catcacgaag tagccgtccg
      121 aagcggccat cgcctggtag ggcgcggtca tcttgttggc ggtgcccagt ggctccggcg
      181 gcttgccggt gccccagtag tcgcaaatgt cccacaccga aaaagccagc gccgaatcga
      241 acagcgcggc gtcgatgtac tggccctgcc ctgtcgcctt cgcgccgatg taggcggcga
      301 gcgtggcgta gatggcaaac agtgcacagc cgatgtcggc caccggcaca cccgccttga
      361 cagggggacc gcccgggtag cccgtcacgc tcatcacgcc ggacatcgcc tgcgccatca
      421 gatcgaagcc cggacggtcg gcccagggcc cggtctggcc gaaaccggag atgctggtgt
      481 aaaccaggcg cgggttcagt gccttcacgt cgtcgtagcc gagccccagg cgcttcatcg
      541 cgcccggacg gtagttctcg accaggatgt cggcatcctt caccagccgc agcagcacgg
      601 cgcgcccgcc ctcggacttg aggttcagcg cgacgctgcg cttgttgcgg ttcatgttca
      661 ggaagcccat gctgtcgggc cccttcatct tgaagcccat ggagccgcgc gtctggtcgc
      721 cgccgccggg aggctcgatc ttgatcacgt cggcgcccag gtccgccaac agcatgcagg
      781 cgtaagggcc ggccatcacc tggctgatgt ccagcacgcg cacgccggcc agcggcaagg
      841 gacggctgcc ctgcgttgcg ctttcggata agtgctgcat tcgtgaaccc tcagcgatag
      901 aagagctgca ccagccctag cccggtgaca ggcacgtagg tcaccaacat cagcacggcg
      961 accagcacgc cgatgaacca gacgttgacc ttgctcactt cccagaccga gcatttcgcg
     1021 atggagctgg caaccatcag cacgctcgcc acgggcggag tctgctggcc gatgcccagg
     1081 ttgatcgtca cgatcaggcc gaagtggacc ggatcgatgc cgacggcgcg caccagcggc
     1141 atcacgatgg gaaccaccag gatgatcgcc gcggccgaat gcaggaacat gccaaggaac
     1201 aggaacagca cgttcagcag ccccagcacc acccatttgt tggaagtgaa gtccgacacg
     1261 gcccgggcca gggcctgggg cgcctgcact tcagtgaggt acgtgcccag cagagcgctg
     1321 gtggcgacga gcaacatgac cacggcggtc tgcacgccgc cttccatcac ggccgcgcgc
     1381 aggtgcttca ggtccagctc gcgatagacg accaggccga cgaacagcgc ggccaccacc
     1441 gccagcgcgg ccccttccgt cgcagtcacg atcccgccga agatgccccc caggatgatg
     1501 atcggcaggg caaaggccca gagcgcctcg cgcccggtcc tgcgcacgcg tgcccacgag
     1561 aaccggtcct cgcgtggcag gttatagcgg cgcgccagga acgtcgccat gcccatcagg
     1621 ccgatgccgc cgatcaagcc cggcacgatc cccgccacga acagctgcac cacggagctt
     1681 tccgccatca ccgcatacag gatcatggga atcgacggcg ggatgatcac cgccagcgtc
     1741 gcggcggatg acatggatgc cgcggcgaac gcgctgggat aacccttggc cttcatgccg
     1801 gggatcaata tcgagccgag tgcggcgacg tcggccacgg ccgatcccga gatctcggcg
     1861 aagaacaggg acgtgccgat cgacacgtgc gacaacccgc cgcgcaccca gccgaacagc
     1921 gcggaggcaa aggcgatcag gcgctgcgag atgccggagg cgttcatgat cgcgcctgcg
     1981 aggatgaaca acgggatcgc cagcagcggg aaattggtcg cgccgttgta gatgaccagc
     2041 ggcatgttgg gcagcgaacc caggccgtgg ctggccacca tcgcgatcac ggcgacgatg
     2101 cccagcgaca cggcgatcgg cacgttggcg agcaccagca ccagcaccga gccgaacagg
     2161 gtgaacatga ttgccatgtg aagccttagg gcctgttcac gctagtgcag gccgtcggcc
     2221 agggccgcgc ccgaacccgc cgcggcgtgg tgccgcgatc ccgccaggtc gatcaggtgc
     2281 atcacctcac cgaccaggat gaggacggcg gaaaccggaa tgaccgactg gaccacgttc
     2341 atcggaatcc agggcaggct gacgagcgct tcgccgacca gcgcgggcat gatcgccacg
     2401 cccatccagc ccagcagggt gaagaacagg atcaccagca cctgcccgaa gatgttcacg
     2461 gccttccgaa agccaggcgg catctggtcc acgatctcag gacacgcgat gtgagcccgt
     2521 ttgacggacg caagcgccga tccgtagaac gtgagccagg ccagcagcac agaggccacc
     2581 tcgtcgtacc acaccaggga actgcccccg gcgcggaata ggacgccgag agtcacttcc
     2641 acggccagcg ccaccatcag gaccatcacc acccattcca ggaacaaacc gtagcggccc
     2701 cgaaaacggt gcatggcgga gccggcgtgc ggcgccatcg tgtcgtgcat gtcgtgctct
     2761 ctacttgccg agcgccacgg ccttgtcgat cagcggcttg gcgccggcga cttccttgcc
     2821 gaactcgtcg tagatgccct tgctggccgc gatgaaggcg ttcttgtcga cctcgttgac
     2881 ctgcatgccc gcttgcttga gcttttccag cagttcggtt tcctgctttg cggcggtcgc
     2941 gtagacgaac gcctgcgttt cgcgcgccgt gtcctcgagc accttgcgca cgtcggccgg
     3001 cagcgtggcg tacttgcgcg aaccgaccag caggtacgcg ggcgtgtaga catggcctga
     3061 cagcgaaagg tacttctgca cttcctgcag cttggccgaa tagatctgcg tgaacgggtt
     3121 ctcctgcccg tccatcacgc cggtctggag cgccgtgaac aattcggaga acttcatcgg
     3181 gctggggttg gcgccgtact cctggaacat cttcacccgc cactttcctt ccggcacgcg
     3241 cagcttgaag cccttgaggt cctcgggcac cttcaccggc ttcttgttgt tggtgatgtg
     3301 gcggtagccg ttctcccaca ccgccagcac tttcaggccc ttcttctcgg tttcgggcgc
     3361 cagggtaggc cagaacacgt ctttctcgat gcgcgccatg tgcgcgcgat ccttcacgat
     3421 gtaaggcagc tcgaagatgc cgaacaggtc cacctccgaa gtcatcacgg tggacggcag
     3481 ggccatttcg atggtgccca gcttgagctt ctggatcatc tccttgtcgc cgccgagctg
     3541 gctggagccg taggtcacga ccttgacctt gccggccagt ttttcgttga cccgcttcgc
     3601 gtactcgtcc gcgctcatct ggaacagcga tccgggttcg cccacgtgcc cgagcttgag
     3661 ttcgacgggc tgcgccacgg cggtgccgtg cagggcgaat gccgcgcctg cgacaaccag
     3721 cagcagcgcc gcaatacgtt ttagtgaatc agcgatcatg ttgtctcctt ctaaaaacca
     3781 accaccactg tcatcccgga ccccggatct agtccggggc gggcctgatc cgggatccac
     3841 gttggcgtgc caaacatgga ttgcgggtca agcccgcaat gacagagcct caggttcgac
     3901 ggaaaacata aggagacatc atgaaagctt tcaccattcc ctcgggccga atcgcgccta
     3961 agctgcgcgc tttcgcgctc gccgcgcttg cggcagccgg ccttgcgctc atc
//
LOCUS       DASWIY010000008         5389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3540, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000008 DASWIY010000000
VERSION     DASWIY010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3540"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..81
                     /locus_tag="VGA59_00405"
     CDS             <1..81
                     /locus_tag="VGA59_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically inducible protein C"
                     /protein_id="HEX9718164.1"
                     /translation="HDKYCSASVMLGKTAEITTSFELVEV"
     gene            complement(320..937)
                     /gene="coq7"
                     /locus_tag="VGA59_00410"
     CDS             complement(320..937)
                     /gene="coq7"
                     /locus_tag="VGA59_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEX9718165.1"
                     /translation="MDRLLGAADSALRTLFAKQHAARACPVVPADETRLDNQERKHAA
                     ALMRVNHVGEVCAQALYTAQALATRDPALRAHFERAAAEETDHLAWTADRLAELGDRP
                     SLLNPLWYAGAFGLGLLAGRLGDRLSLGFVVETERQVEAHLQSHLDRLPAADHASRAI
                     VVQMKDDEAAHAAQALQAGGVELPAPAKVLMRAAAKVMTTTAHYV"
     gene            1255..2307
                     /locus_tag="VGA59_00415"
     CDS             1255..2307
                     /locus_tag="VGA59_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX9718166.1"
                     /translation="MKKSLIALAVLATAGVASAQSSVTLFGIVDAAITVGRGNVADRT
                     QLTNSGYNSSRLGFRGTEDLGGGMSASFWLEAALQNDNGAGLGTNTNNQATGATTGNG
                     LTFGRRSTVSLAGGWGEVRLGRDYTPQFWNLTVFDPFGTNGVGTTQLLSSIITGVTAV
                     RASNSIGYFAPGKLGGFYGQVQYYMGENLQNGAATEDDGRGLGLRVGFANGPFNVALA
                     MSRTDYNAGDVQQANIAGSWNFGMAKLMGQFSRDKNDALNATGKGALIGALVPVGAGE
                     IRVAVSQYKVNVAGDPKTTKVALGYVHNLSKRTAVYTTIARVKNKGGAAQALGGSVTS
                     ANSSSTGFDLGVRHSF"
     gene            2491..2910
                     /locus_tag="VGA59_00420"
     CDS             2491..2910
                     /locus_tag="VGA59_00420"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEX9718167.1"
                     /translation="MASRESDDSPVRVVLVFEGDRARFSARDAMLSELVHALTGEEMP
                     YATLMYVWCNRRAPGTVMTSPRTDRIRTIVVESGPTQLDRWLDYERDIHSDYRRAFGE
                     APGALIGIGIMTDSDNTHTTAQAWYGPVRLGNSDAGR"
     gene            3028..4008
                     /locus_tag="VGA59_00425"
     CDS             3028..4008
                     /locus_tag="VGA59_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9718168.1"
                     /translation="MLAFVLRRLLQAVIVMVTVAFIAFMLFQYVGDPVVFLLGQDAKP
                     DQISQLRADLGLDQPFFVQFWHFLLNAAQGEFGLSLRQGAKVSRLIGERFPATLELSL
                     VAAVGAVVIGIPMGVYAALRRGSFMSQVFLTISLLGVSLPTFLIGILLILVFAVLLGW
                     FPSFGRGDVVSLGWWNTGLLTADGWHHIVLPAVTLAIFQLTLIMRLVRSEMLEVLRTD
                     YIKFARARGLPDRAIHFGHALKNTLVPVMTIIGLQLGTLIAFSIITETVFQWPGMGLL
                     FIQAVTFADIPVMAAYLCLIALIFVVINLVVDLLYFAVDPRLRVGSAGGH"
     gene            4008..5219
                     /locus_tag="VGA59_00430"
     CDS             4008..5219
                     /locus_tag="VGA59_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX9718169.1"
                     /translation="MLAPRLKVNGRAFAHIAQFHPELTALRRDLHAHPELGFEEIYTS
                     SRVKESLKLCRVDEIHTGIGRTGVVAAIKGQRTSSGRMVGLRADMDALTMTEQNDFAW
                     KSSKHGMMHGCGHDGHTAMLIGAARYLAETRNFDGTAVLVFQPGEEGFAGAEQMIEDG
                     LFQRFPVEAIYAMHNWPAMKPGTVGINPGPMMAAADRVTIEVTGRGGHGAHAYLATDP
                     VLVAAHIITAVQSIVSRNVRPIDGAVISLCAMHAGDPGAMSVIPDKATLVGTVRTFQP
                     EVQALIERRLAELCSAIALGLGATATVRYERIYPATINSVAEANFAADVAESLVGADH
                     VVRDMEPSMGAEDFSFMLQTKPGAYMRIGQGGEGSCFLHNGRYDFNDDILPIGSALHA
                     SLIEQSMPLST"
BASE COUNT          919 a         1763 c         1723 g          984 t
ORIGIN      
        1 cacgacaagt actgctcggc cagcgtcatg ctgggcaaga cggccgagat cacgacgagc
       61 ttcgaactcg tcgaagtcta gtgccgctgt ccgtgaactt gtcatcccgg gcttgaccaa
      121 gttgtcatcc cgggcttgac caagttgtca tcccgggctg ggctcgcgga acgcccgggg
      181 cgcgcagctg tccagtgcag cgagcccaat gccgggcctg cgctatgcac gtgccgaggc
      241 attgacgctc cggcccggcc ccgggatcca tggattgcgg atcgagtccg caatgacagg
      301 caagactcaa cgctcgacgc tacacgtaat gcgcggtggt tgtcatcacc ttggcggcgg
      361 cacgcatcag gaccttggcg ggcgccggca gttcgacccc gcctgcctgc agtgcctggg
      421 cggcatgcgc cgcctcgtcg tccttcatct gcaccacaat ggcgcgggag gcatggtcag
      481 ccgccggcaa acggtcaagg tgactttgca ggtgggcttc aacctgccgt tcggtttcga
      541 cgacgaagcc caggctgagg cgatcgccga gccgaccggc caggaggccc aggccaaaag
      601 cgcccgcata ccatagcgga ttaagcagtg aaggtcgatc gcccaattca gccaagcggt
      661 ccgccgtcca cgccaggtgg tccgtttcct cggccgccgc ccgctcgaaa tgggcgcgca
      721 gcgcgggatc gcgcgtggcc agggcctggg ccgtatacag tgcctgggcg cagacctccc
      781 ccacgtggtt cacacgcatc agcgcggcgg cgtgttttcg ctcctggttg tccagccggg
      841 tttcgtcggc cggcacgacg gggcaggcgc gagccgcgtg ttgcttcgcg aaaagggttc
      901 tgagggcgct gtcggcggcg cctagcaaac ggtccatggg ccctagttta cgtccgcgcg
      961 aggggtgcca cgggcggcgc tttcaagctg cgaattcagg gttggatttt cgctatccga
     1021 aacgatggcg ggtagtgttg cgcgggttcc actgggccaa ttgcgtgtgt tgtccgggtg
     1081 caacgaaacc gcctatgaaa ggctaaatac tttgcgaaag aaaggccgct ctggtgcaat
     1141 aacgacaact tcccaaaagg aggttggccc ggggatccgg cgggagcaca aaagtgcctg
     1201 catttcgtga ccccttcgca ccggagccct atgtttaacc ttggagaaac ttgcatgaaa
     1261 aagtccctga ttgctctggc cgtgctggca accgccggcg tggcttctgc ccaatcttcc
     1321 gtcacgctgt tcggtattgt cgacgcggcc attacggtcg gccgtggcaa cgttgcagac
     1381 aggacccagc tgaccaactc gggctacaac agctcgcgtc tcggcttccg tggcacggaa
     1441 gacctgggcg gcggcatgtc cgcctcgttc tggcttgaag ccgccttgca aaacgataac
     1501 ggcgcaggtc tcggcaccaa caccaacaac caggcaacgg gcgccaccac gggcaacggc
     1561 ctgacgttcg gtcgccgttc gacggtgagc ctggccggcg gctggggcga agtgcgcctg
     1621 ggtcgtgact acaccccgca attctggaac ctgaccgtgt tcgacccgtt cggcacgaac
     1681 ggcgtgggca ccacccagct gctgagctcc atcatcaccg gtgtgaccgc ggtgcgcgcc
     1741 tccaacagca tcggctactt cgcgccgggc aagctgggcg gcttctacgg acaggtccag
     1801 tactacatgg gtgagaacct gcagaacggc gcagccacgg aagacgatgg cagaggcctt
     1861 ggcctgcgcg ttggtttcgc caacggcccg ttcaacgtgg cgctggccat gagccgcacc
     1921 gactacaacg cgggtgacgt ccagcaggcc aacatcgctg gctcgtggaa cttcggcatg
     1981 gccaagctga tgggccagtt cagccgcgac aagaacgacg ccctgaacgc gaccggcaag
     2041 ggcgccctga tcggcgcgct ggttccggtt ggcgcgggcg aaatccgcgt ggcagtgtcg
     2101 cagtacaagg tcaacgtggc gggcgacccc aagaccacca aggtggctct gggctatgtg
     2161 cataacctgt ccaagcgcac ggctgtgtac accacgattg ctcgcgtgaa gaacaagggc
     2221 ggcgcagcgc aggccctggg cggttccgtc acctccgcga actccagctc gacgggcttc
     2281 gacctcggtg tccgtcacag cttctaaaga cctcggctgg ccgcgcgcca gcttaggctg
     2341 aagaaacttg aaaagccgcc ttcgggcggc ttttttatga gtgcgcccgg cagggcgcac
     2401 ccacttggag gtgaaagtcc tctactcgcc cggcaggggg aagagttagc ccaaggcaag
     2461 ggtttcgcgg gcgactgcga gtctgcggac atggcctcgc gggagagcga cgattccccg
     2521 gttcgcgtcg tgctggtctt cgagggtgac cgcgcccgct tttccgcgcg cgacgcgatg
     2581 ctctccgaac tggttcacgc gctgacgggg gaggagatgc cctacgcgac gctgatgtac
     2641 gtgtggtgca accggcgcgc acccggcacg gtgatgacca gcccgcgcac cgaccgcatc
     2701 cgcacgatcg tggtggagtc cggccccacg cagctcgacc gctggctgga ctacgagcgc
     2761 gatatccaca gcgactaccg gcgtgcgttc ggcgaggcac ccggtgcgct cataggaatc
     2821 ggcatcatga ccgacagcga caatacccac accactgcgc aggcctggta tgggcctgtc
     2881 cgcctgggca attcagacgc cggcagatag gcacggcgcc cgtcctgagc cgtgtcggcg
     2941 gccggccgat cctttaggcc tgaaggaaac ccctgtgtcg gcccgaccct tgctctgcta
     3001 gcatcccctc tgcatttttt gcgctcaatg cttgcctttg ttcttcgccg cctgctccag
     3061 gctgtgatcg tgatggtcac ggtcgcattc atcgcgttca tgctgttcca gtacgtgggt
     3121 gacccggtgg ttttcctgct cgggcaggac gccaaacccg accagatcag ccagttgcgc
     3181 gccgaccttg ggctggatca gccctttttc gtgcagttct ggcatttcct gctcaacgcg
     3241 gcccagggcg agttcgggct cagcctgcgc cagggcgcca aggtttcgcg gctgatcggg
     3301 gaacgatttc cggccacgct cgaactgtcc ttggtggcgg cggtcggagc cgtcgtcatc
     3361 ggcattccca tgggcgtgta cgcggccttg cggcgcggca gcttcatgag ccaggtgttc
     3421 ctcacgatct cactgctggg cgtgtcgttg cccacctttc tcatcggtat cctgctgatc
     3481 ctcgtgttcg ccgtgctgct gggctggttt cccagtttcg gccgcggcga cgtggtgtcg
     3541 ctgggctggt ggaacaccgg gttgctcacg gccgatggct ggcaccacat cgtgctgccg
     3601 gccgtgaccc ttgccatctt ccagctcacg ctgatcatgc ggctggtgcg ctctgaaatg
     3661 ctggaagtgc tgcgcaccga ctacatcaag ttcgcccgtg cccgcggatt gcccgatcgc
     3721 gccatccact tcggccatgc gctcaagaac acgctggtgc cggtcatgac gatcatcggc
     3781 ctgcaattgg gaacgctgat cgcgttttcg atcatcaccg agaccgtatt ccagtggccc
     3841 gggatgggtc tgctgttcat ccaggccgtg acctttgccg acatccccgt gatggccgcc
     3901 tacctgtgcc tgatcgcgct gatctttgtc gtgatcaacc tggtggtgga cctgctgtac
     3961 ttcgcggtcg atccccggtt gcgcgtgggc agcgcggggg gacattgatg ctcgcacccc
     4021 gcctgaaggt caacggccgc gcgttcgccc acatcgcgca gttccacccc gaactcacgg
     4081 cgctgcggcg cgacctgcat gcccaccccg aactcggctt cgaggagatc tatacctcga
     4141 gccgcgtgaa ggagtcgctc aaactgtgcc gcgtggacga gatccatacc ggcatcggca
     4201 ggacgggagt cgtcgcggcc atcaagggcc agcgcacttc gagcgggcgc atggtgggtc
     4261 tgcgcgccga tatggatgcg ctcaccatga ccgagcagaa cgacttcgcc tggaaatcgt
     4321 cgaagcacgg catgatgcac ggctgcggcc acgacggcca caccgccatg ctgatcgggg
     4381 cagcgcgtta cctcgccgaa acccgcaact tcgacggaac ggcggtactg gtgttccagc
     4441 ccggcgagga aggcttcgcc ggcgccgagc agatgatcga ggacgggctg ttccagcgct
     4501 ttcccgtcga ggccatctac gcgatgcaca actggccggc gatgaaaccc ggaaccgtgg
     4561 gcatcaatcc cggacccatg atggcggcgg ccgaccgcgt caccatcgaa gtcacgggcc
     4621 gtggcggtca cggcgcccat gcctacctgg cgaccgaccc cgtcctggtg gcggcccaca
     4681 tcatcaccgc ggtgcagagc atcgtatcgc gcaacgtgcg gccgatcgac ggcgccgtga
     4741 tcagcctgtg cgcgatgcat gccggcgacc cgggagcgat gagcgtcatc ccggacaagg
     4801 ccaccctggt cgggacggtg cgaaccttcc agcccgaagt ccaggcgctg atcgagcggc
     4861 gcctggccga actgtgcagc gcgatcgccc tgggcctggg cgcgacggcc acggtgcgtt
     4921 acgagcgcat ctatccggcc accatcaatt cagtggccga agccaacttc gcggccgacg
     4981 tggctgaaag cctggttggg gccgatcacg tggtgcgcga catggagccg agcatgggcg
     5041 ccgaagactt ttccttcatg ctgcagacca agccgggcgc ctacatgcga atcgggcagg
     5101 gcggcgaggg cagctgtttc ctgcacaacg gccgctacga tttcaacgac gacatccttc
     5161 cgatcggatc ggccctgcac gccagcctga tcgagcagtc catgccgctt tccacctgac
     5221 gacccccttt ttttgaggag accctgatga aattcaagcc cactctgctt tctgccgccg
     5281 tgttcgccgc tcttggcgtc atcagccttg cggccggtgc cgcgacgctg aaaatcgcga
     5341 accagggcga cgccctctcg ctggaccccc attcgctgaa cgagtcgct
//
LOCUS       DASWIY010000009         4012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_372627, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000009 DASWIY010000000
VERSION     DASWIY010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372627"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VGA59_00435"
     CDS             complement(<1..927)
                     /locus_tag="VGA59_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HEX9718170.1"
                     /translation="MAVFSTSGTATGAAAQQAFKKQLNKVYAFYTGGFITFVIVLAIL
                     EQFGLKKEWIGFIFLMATIGLYAGIGIMSRTTDAAEYYVAGRRVPAVYNGMATGADWM
                     SAASFIGMAGTLYLTGYGGLAFIMGWTGGYCLVALLLAPYLRKFGQFTIPDFLGERYG
                     GNLPRFIGMLAAILCSFTYVVAQIYGVGIITTRLTGVAFELGIFLGLGGILVCSFLGG
                     MRAVTWTQVAQYIILIMAYMIPVVWLAVKQTGVPVPQAIYGYQLDKVAKRGDQLRTDP
                     KELEVVAIFKARADEFDAKLKNIPTALATDRAA"
     gene            complement(931..1188)
                     /locus_tag="VGA59_00440"
     CDS             complement(931..1188)
                     /locus_tag="VGA59_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEX9718171.1"
                     /translation="MQLTEGHRLYWRKNLTITGILLVIWFVVTYVVGFFARDLNFSFF
                     GWPFSFWMGAQGSLIIYVVIIWYYARYMNKLDQEFGVAEEE"
     gene            complement(1293..1871)
                     /locus_tag="VGA59_00445"
     CDS             complement(1293..1871)
                     /locus_tag="VGA59_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9718172.1"
                     /translation="MMRGLRYRGWKWRAIALLFFGRQPAALSLFEAMLQEFPGDSYAL
                     ASKAHLQARLGRRRDALETYRLLVEIDAANGTHWFNYGFLLEELDEYGQAEAAFRRAT
                     ELSPKLDRAWYGLGLVLIRLQRFDEAIVALKKNTQLQPMSPYGWYQLARAHVDRHEPD
                     EALRIIRHLKRFEPKVAAQLERETGIFVPPAS"
     gene            complement(1868..2164)
                     /locus_tag="VGA59_00450"
     CDS             complement(1868..2164)
                     /locus_tag="VGA59_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718173.1"
                     /translation="MLTLVSIVKLLAEIALLALLGQGVLALLAGAKRDRNLFYQMLQV
                     MTRPFIAGARFITPKVVIDRHVPIVAFLLLFFIWLVATITKISICVQIGVEKCT"
     gene            2377..2925
                     /locus_tag="VGA59_00455"
     CDS             2377..2925
                     /locus_tag="VGA59_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718174.1"
                     /translation="MEPGAKVIQMLQKNLVYHKSAKGTEAIATRQHGLSPKLRSTLIL
                     IDGKRGFEELARLSQALGDTEQLLGQLLEQGFIEEVVGAPAAPAAAPTKSAAAPAKTA
                     AAPAAGRGLPLLDAQRYASKRLFAILGVSAEPVCLRIEAARNAQDFQAAIKLAEIMVS
                     DVRSGRVAEEFAAEMQEHMPGA"
     gene            complement(2966..>4012)
                     /locus_tag="VGA59_00460"
     CDS             complement(2966..>4012)
                     /locus_tag="VGA59_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HEX9718175.1"
                     /translation="REPSAARELWTRAMHESHDRSQPLGGLLPHSQPFAGDPDGTPAQ
                     RDLFDTSRRNFLALVFCLMVGTAGLPHLLTRFYTTPTVGEARSSVVWALFFIALLYLS
                     VPALAVLVKYEVMQGLVGSSFDALPSWILQWGKVDASLISVSDVNGDGIVQFGEIRLG
                     ADIIMLATPEIGGLPYVVSGLVAAGGLAAALSTADGLLLTISNAMVRDVYCEEIGHHV
                     TPEQRVILSKFALLAVALVAAMVASLKPADILPLVSASFSLAASAFVPAMVLGIFWPG
                     ATRAGAVAGMAAGLGITVYYMLVNAPSVRAILKLSPEPQLWLGIVPVSAGVFGVGLGL
                     AVALVVSLLTLRAD"
BASE COUNT          806 a         1315 c         1257 g          634 t
ORIGIN      
        1 ggccgccctg tccgtggcca gtgccgtcgg gatgttcttc agcttcgcat cgaactcatc
       61 ggcacgggcc ttgaagatcg ccacgacctc gagttctttc gggtccgtgc gcagttggtc
      121 cccgcgcttg gctaccttgt cgagctgata gccgtaaatg gcttgcggaa cgggcacgcc
      181 ggtctgcttc accgccagcc acaccacagg aatcatgtag gccatgatca agatgatgta
      241 ctgcgccacc tgggtccagg tcacggcgcg cataccgccc aggaacgagc agaccaggat
      301 gccgcccaaa cccagaaaaa tgcccagctc aaaggccacg cctgtgaggc gcgtcgtgat
      361 gatgcccacg ccatagatct gggccaccac ataggtgaac gagcacagga tcgcggcgag
      421 catgccgatg aaacgcggca agttgccgcc gtagcgctcg cccaggaagt ccggaatcgt
      481 gaactgcccg aacttgcgca ggtaaggcgc cagcagcagg gccaccaggc aatagccgcc
      541 ggtccagccc atgatgaagg caaggccgcc gtacccggtc aggtacaggg ttccggccat
      601 tccgatgaac gacgcagcgg acatccagtc cgcgcctgtc gccatgccgt tgtagacggc
      661 tggtacccgc cggcccgcga catagtattc ggccgcgtcc gtcgtgcggc tcatgatgcc
      721 gatgccggcg tacaggccga tcgtcgccat caggaagatg aagccgatcc attccttctt
      781 gagcccgaac tgctccagga tcgcaaggac gatgacgaag gtgataaagc ccccggtgta
      841 gaaggcatag accttgttca gctgcttttt gaaggcttgt tgcgccgcag ccccagtggc
      901 ggtgccgctt gttgagaata cagccatgac ttattcctcc tcggccacgc cgaattcctg
      961 gtccagtttg ttcatgtagc gcgcgtagta ccagatgatc acgacataga tgatcaacga
     1021 gccctgcgcc cccatccaga agctgaacgg ccagccgaaa aagctgaaat tcaggtcgcg
     1081 ggcaaaaaag cccaccacgt aagtcaccac gaaccagatc accagcagga tgccggtgat
     1141 ggtcaggttc ttccgccagt acaggcggtg tccttctgtc agttgcatgc tgaatgtctc
     1201 ctgttatgcg ccctcgtggg cctctctctg ccaccaaacg gcccgccggc gaccgccagc
     1261 ttgcaaccgg gccgtgcccg gtaaataccc cctcagctgg cgggcgggac aaaaatcccc
     1321 gtctcgcgtt ccagctgagc ggctactttc ggctcgaacc gtttgaggtg gcggatgatc
     1381 cgcagggcct cgtccggttc gtgccggtcg acatgagccc gcgcaagctg gtaccagccg
     1441 tacgggctca tgggctgcaa ttgggtgttt ttcttcagcg cgacgatggc ctcgtcgaag
     1501 cgctggagcc ggatgaggac caggcccagg ccgtaccagg cgcgatccag cttggggctg
     1561 agctcggtgg cgcggcggaa cgcggcctcg gcctgcccgt actcgtccag ctcctccagc
     1621 aggaagccgt agttgaacca gtgcgtgccg tttgcggcgt cgatctccac cagcaggcgg
     1681 tatgtctcca gcgcatcgcg acggcgcccg aggcgggcct gaaggtgtgc cttgctggcc
     1741 agcgcgtagc tgtcgccggg aaactcctgc agcatggctt cgaacagcga cagggccgcc
     1801 ggctggcgcc cgaagaacag cagcgcgatg gcgcgccact tccatccgcg gtagcgaagc
     1861 ccgcgcatca tgtgcacttc tccaccccga tctggacgca gatgctgatc ttggtgatgg
     1921 tggcgaccag ccagatgaaa aacagcagca ggaaggccac gatcgggaca tgccggtcga
     1981 tcaccacctt gggtgtgatg aaacgcgcgc cggcgatgaa aggccgggtc atgacctgca
     2041 gcatctggta gaacaggttc cgatcccgct tggccccggc cagcagcgcc agcacaccct
     2101 gccccagcaa ggccaggagg gcgatctcgg ccaagagctt gacgatgctg acgagagtga
     2161 gcaacctgac tcccacgtga tgaagggtgc atcgtttgca ggcgcgctta ctcgcccctt
     2221 acaaaagcat ccatgcgcga ggtaaacccc ctcagggatt acccggcgac cgcccaagcc
     2281 gggccgcgcg gggatgggta gtgttgcggc gctgcaattg aggtaaaact cccactcgac
     2341 gacaagcgtc tgatgtgctt acactaaact gacaaagtgg aaccgggggc taaagtgatt
     2401 cagatgcttc agaagaacct tgtctatcac aagtcggcca agggcaccga ggccatcgcg
     2461 acgcgccagc acgggctcag ccccaagttg cgctcgacac tgattctgat tgacggcaag
     2521 cgcggtttcg aggaactggc gcgcctgtcg caggcgctgg gcgacacgga acaactgctg
     2581 gggcaattgc tcgagcaggg ctttatcgag gaggtggttg gggcccccgc ggcgccggcg
     2641 gcagcaccaa cgaaatccgc cgcggcgccg gcgaagaccg cggcagcgcc cgcggccggg
     2701 cgggggcttc ccttgctcga cgcgcaacgt tacgcatcaa agcggctgtt cgccattctc
     2761 ggcgtcagcg ccgagccggt ttgcctgcgc atcgaggccg cgcgcaacgc ccaggacttt
     2821 caagccgcga tcaagctggc cgaaatcatg gtgagcgatg tccgcagcgg cagggtcgcg
     2881 gaagaattcg ccgctgaaat gcaggagcac atgcctggcg cctagccggg tccccgaccg
     2941 aggccggcaa gacggcaccg tccattcaat cggcacgcag ggtgagcagg ctgaccacca
     3001 gcgcgaccgc gagtcccagc cccacaccga atacgccagc cgaaacagga acgatgccca
     3061 gccagagctg cggctcgggc gacagcttga gaatcgcccg caccgaaggc gcgttgacga
     3121 gcatgtagta caccgtgatg cccagtcccg ccgccatccc ggccacggcg ccggcgcgcg
     3181 tggcaccggg ccagaagatt cccagcacca tcgccggcac gaaggcggaa gcggccaggg
     3241 aaaacgaggc cgagaccagc ggcaggatgt ccgccggctt gagcgaggcc accatggcgg
     3301 ccaccagcgc caccgccagc agggcgaact tggacaggat cacgcgctgc tcgggcgtga
     3361 catgatggcc gatctcctcg cagtacacat cgcgcaccat cgcattgctg atggtcagca
     3421 acagtccgtc ggcggtcgac agtgccgccg ccagcccgcc ggcggcgacc aggcccgaga
     3481 ccacatacgg gagcccgccg atctccggcg tcgcgagcat gatgatgtcg gcgccgagcc
     3541 gtatttcgcc gaattgcact atgccgtcgc cgttcacgtc gctgaccgaa atcagcgatg
     3601 catccacctt tccccattgc aggatccagg atggaagcgc gtcgaaactg cttcccacca
     3661 ggccctgcat gacttcgtac ttcaccagca ccgccagcgc cggcacgctc aggtacagca
     3721 aggcaatgaa aaacagcgcc cagaccaccg acgagcgggc ctcgccaacc gtgggcgtgg
     3781 tgtagaagcg ggtcagcaga tgcggcaggc ccgcagtccc gaccatgagg cagaaaacca
     3841 gcgccaggaa attacgccgc gacgtatcga aaaggtcgcg ttgcgccggc gtgccgtccg
     3901 gatcacccgc gaatggctgg ctgtgcggca gcaggccccc caggggctgc gacctgtcat
     3961 ggctctcgtg catggcgcgc gtccagagtt cgcgcgccgc cgacggctca cg
//
LOCUS       DASWIY010000010         9063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2222700, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000010 DASWIY010000000
VERSION     DASWIY010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9063
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222700"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..776)
                     /gene="purN"
                     /locus_tag="VGA59_00465"
     CDS             complement(195..776)
                     /gene="purN"
                     /locus_tag="VGA59_00465"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX9718176.1"
                     /translation="MKNIVILISGGGSNMAAIVRSAQREGWDRSRDARVAAVISNRAD
                     AGGLVFAREQGIATDVVDHKGHATRAAFDAELAAAIDRHQPTLVVLAGFMRILTPGFV
                     QRYQGRLLNIHPSLLPAFPGLHTHQRAIDAGCKVAGATVHQVTSELDYGPILAQAVVP
                     ILPGDTAATLAARVLTQEHLIYPRAIAELLPSL"
     gene            838..1503
                     /locus_tag="VGA59_00470"
     CDS             838..1503
                     /locus_tag="VGA59_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEX9718177.1"
                     /translation="MPLRPLTPIEARVLATLMEKARTVPDSYPLSLNTLVAGCNQKTS
                     REPLMNVTEAQAQEALDALKLLSLVFQSSGSRVARWEHNFQRGVGVPEQSAVLLGLLM
                     LRGPQTAGELRINCERWYRFADISSVEAFLEELQDRPEEKGGPLVVKLPRAPGMREQR
                     WAHLLSGPVEVGPEPAHAQPGEGQHGLEGRVEVLENEVARLRSSVQNLYEQLGLSQPG
                     QAE"
     gene            1545..2744
                     /locus_tag="VGA59_00475"
     CDS             1545..2744
                     /locus_tag="VGA59_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9718178.1"
                     /translation="MDLAFTPEELKFREDVRAWVRANLPKEISHKVHNSLRLTRDDMQ
                     GWSRILGKRGWLGYGWPKQFGGPGWTAVQKHLFEEECALAGAPRIVPFGPVMVAPVIM
                     AFGTPEQQQRFLPGIASGEVWWSQGYSEPGSGSDLASVKCRAERKGDKYIVNGQKTWT
                     TLAQYGDWIFCLVRTSTEGKPQTGISFLLIDMKSPGVSVRPIYMLDGEPEVNEVFFDN
                     VEVPAENLIGEENKGWTYAKHLLSHERTNIADVNRAKRELERLKRIAKAEGMYDPAEG
                     GDNARRFREEIARLEVDVVALEMLVLRVLSAEKSGKNSLDIAGLLKIKGSEIQQRYSE
                     LMMLAAGPYALPLIQEAMEAGWQGEYVGAAHCAPLASTYFNFRKTTIYGGSNEVQRNI
                     VAQTVLG"
     gene            2842..3957
                     /locus_tag="VGA59_00480"
     CDS             2842..3957
                     /locus_tag="VGA59_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9718179.1"
                     /translation="MDFDFTNDQEQLRDAVRKWVDKGYDFERRRAIVKAGGFDKDAYS
                     ELAQLGLTGLYVPEDQGGMGMGPVEGMVVMEELGRGIVLEPLAQTLIAGGILNGYADD
                     AVKSAWLPRIAGGEALVVLAQQERKARYRLDMCETSATRSGGAWAVTGAKSIVAAGDR
                     ADAFLVPATVDGKIALFLVERSAAGVTAKGYGTQEGGRAAELGFKSAPATLVTLDGLT
                     ALEHAVDIGIAASCAEAVGVLDKTMDVTVEYMNTRKQFGVTLSTFQALRHRVADMKMQ
                     LELARSMSYYASLKLNAPTEERRRALARAKYQLGVAMRFIGQQSVQLHGGIGVTDEYI
                     GSHYFKKLTQLELSFGDTLHQLGEVSNRMQDTAGVFA"
     gene            complement(4271..4828)
                     /locus_tag="VGA59_00485"
     CDS             complement(4271..4828)
                     /locus_tag="VGA59_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX9718180.1"
                     /translation="MKVLKLSAQGLPQSWISLEQAVLHYAADEVRWEVGAQVALFRGG
                     HNAMTGAQSQIAVNSIIGTKGVPNINPFDLRPGLTNGKLFARDRNVCAYCGHLYHEED
                     LTREHIIPFAQNGRDHWMNVVTACRTCNHRKSNRTPEQARMPLLYAPYIPSLWEDFIL
                     RNRRILADQMEFLMAHLPRSSRLHA"
     gene            5002..6024
                     /locus_tag="VGA59_00490"
     CDS             5002..6024
                     /locus_tag="VGA59_00490"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX9718181.1"
                     /translation="MQIFRGFHHPGIAPACALTIGNFDGVHRGHQAMLALLNNEARHR
                     NVPSCVLTFEPHPRDYFAAIARKPELAPARIATLRDKLTELARCGVDQCVVLSFDARL
                     SSQTPEAFIDNVLVRGLGVRYVLVGDDFRFGAKRAGDYTTLDAAGAARGFDVARMNSY
                     EVHGVRVSSSEVRDALGRGDMDGVTALLGRPYSISGHVVHGRKLGRQLGFKTVNLRFS
                     HWKPAAGGIFAVRVHGLEGPAQPDGADSSLPGVANLGIRPSLNPDDVNGGRVLLESHC
                     LEWPPSLGEEGAYGKIIRVELLHKLHDELKYESLDALKAGIARDSDQARAYFASTRRQ
                     TTRDRI"
     gene            6133..>9063
                     /locus_tag="VGA59_00495"
     CDS             6133..>9063
                     /locus_tag="VGA59_00495"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX9718182.1"
                     /translation="MTDKTDYRATLNLPDTPFPMRGDLPKREPGWVKEWEDKGLYKKL
                     RAARCEHEKFVLHDGPPYANGQIHMGHAVNKILKDMIVKARQLKGLDAAYIPGWDCHG
                     LPIENAIEKKYGRNLPRDEMQAKSRAFATEQIGVQMADFKRLGVLGDWDHPYRTMEFI
                     NEAEEIRAFKRVIERGFVYRGLKPVYWCFDCGSSLAEFEIEYADKKSQTLDVGFEADE
                     PQKVLAAFGLPSPQPSPASGRGSEPQLSAGRGSESQLSAGGGSNAHDSADRDSLPLPS
                     GERAGVRGTPTQPIYVVIWTTTAWTIPANQALNLNPDLEYSLVETGRGLLLLAASLVG
                     RCLERYELTGKVLATVKGDKLAGLNFQHPLHDADPGYRRLSPVYLADYATAEDGTGIV
                     HSSPAYGLDDFQSCVAHGMAYDDILNPVQGNGSYAPDFPLFGGLNIWKATPRIIEALR
                     AAGRLFATENIAHSYPHCWRHKTPVIYRAAAQWFIRMDEGDGVFTKDKAPKTLRQMAL
                     EAIEETNFFPENGKARLRDMITGRPDWCISRQRSWGVPLPFFLHKDTGELHPRTMEIL
                     DTAAEMVEQGGIEAWSKATAEAILGPEDAAHYTKSTDILEVWFDSGTTHTTVLKHSHA
                     GAGHESGPEADLYLEGHDQHRGWFHSSLLTACAMYGRAPYRGLLTHGFTVDSQGRKMS
                     KSQGNGVDPQETTKKLGAEIIRLWVAASDYSGDIAGDDKILARVVDAYRRIRNTLRFL
                     LANVSDFDPARDAVALDQMLEIDRYAITRAAQFQAEVLAHYEVYEFHPVVAKLQVYCS
                     EDLGAFYLDILKDRLYTTAPKSLARRSAQTALWNITHAMLRWMAPFLSFTAEEAWKLF
                     GDSESIFFETYAEIGGPDTELLDKWTRIRAARELVNKEIESLREAGKVGSSLQANVQL
                     TVPMATHGELWSALASLGDDLKFVFITSVMRVVAGDVMAVKVTPSEAVKCERCWHWRN
                     DVGQD"
BASE COUNT         1686 a         2906 c         3026 g         1445 t
ORIGIN      
        1 atgttcgaac ttgagggtga gcttgacgag ttcggcgcag gcgtcgagca gggctttggg
       61 atgcatggag cgattgtccc attgagcctt gccacggctg tgcagccgcg atagcgctgt
      121 catgccgggc tcgacccggc atccatgaat tcgggatcat ggattgcggc tcgaggccgc
      181 aatgacagaa cttgctacag actcggcagc agttccgcga tcgcgcgggg ataaatcagg
      241 tgttcctgcg tcagcacccg ggcggccagc gtggcggccg tgtcgccagg caggatgggc
      301 accacggcct gggccaggat gggcccgtag tcgagctcgg acgtcacctg gtgaactgtg
      361 gccccggcca ccttgcaacc ggcatcgatg gcccgctggt gcgtgtgcaa gcccggaaaa
      421 gcgggcagca gggaaggatg gatgttgagc aggcgcccct ggtagcgctg gacgaaaccc
      481 ggcgtgagga tgcgcatgaa acccgccagc acgaccaggg tcggctggtg ccggtcgatc
      541 gcggcggcca gttcggcgtc gaaagccgcg cgcgtggcat ggcccttgtg atccaccaca
      601 tccgtggcga tgccctgctc gcgcgcaaac accagcccgc ccgcgtcggc ccggttgctg
      661 atgaccgcgg cgacccgggc gtcgcggctc ctgtcccagc cctctcgctg ggccgatcgc
      721 acgatggcgg ccatgttgga gccgccgccg gagatcagaa tgacgatgtt tttcattttc
      781 ccaaaccctt gcggaacagg cacccgcgct gcccgcgact gatcgattat ccctgccatg
      841 cccctgcgcc ccctgacccc tatcgaagcc cgcgtgctgg ccacgctgat ggaaaaagcc
      901 cgcaccgtgc cggacagcta tccgctgtcg ctcaacacgc tcgtcgccgg ctgcaatcag
      961 aagaccagcc gcgagccctt gatgaacgtg accgaggccc aggcccagga ggcgctggat
     1021 gcgctcaagc tgctgagcct ggtgttccag tccagcggca gtcgggtggc gcgctgggag
     1081 cacaacttcc agcgcggcgt gggcgtgccg gagcagtcgg ctgtgctgct gggcctgctg
     1141 atgctgcgcg gcccgcagac ggccggggaa ctgcgcatca actgcgagcg ctggtatcgg
     1201 tttgccgaca tctcatcggt cgaggctttc ctggaagagc tgcaggacag gccggaagaa
     1261 aaaggcgggc cgctggtggt gaagctgccg cgcgcgccgg gcatgcgcga gcagcgctgg
     1321 gcccacttgc tgagcggacc ggtcgaggtg ggtccggagc cggcccacgc acagccgggc
     1381 gaaggacagc acggcctcga aggacgcgtc gaagtgctgg aaaacgaagt ggcccggctg
     1441 cgttcctctg tacaaaatct ctacgagcag ctcgggctgt cgcaacccgg acaggctgaa
     1501 tagcacgacc gtgctgataa tcggaccgaa ctggagacgc tctcatggat ttggctttta
     1561 cccctgaaga actgaagttc cgcgaagacg tgcgcgcctg ggtgcgcgcg aatttgccca
     1621 aggaaatttc ccacaaggtg cacaactcgc tgcgcctgac gcgcgacgac atgcagggct
     1681 ggtcgcgcat tctcggcaag cgaggctggc tgggctacgg atggcccaag cagttcggcg
     1741 ggcccggctg gaccgccgtg cagaaacacc tgttcgagga agaatgcgcg ctggccggcg
     1801 ccccccgcat cgtgcccttc ggcccggtga tggtcgcacc cgtgatcatg gcgttcggca
     1861 cgcccgagca gcagcagcgc ttcctgcccg gcatcgccag cggcgaggtg tggtggagcc
     1921 agggctacag cgagccgggc tcgggctcgg acctggcgtc ggtcaagtgc cgtgccgagc
     1981 gcaagggcga caagtacatc gtcaatggcc agaagacctg gaccacgctg gcgcagtacg
     2041 gcgactggat cttttgcctg gtgcgcacca gcaccgaggg caagccgcag accggcatct
     2101 ccttcctgct gatcgacatg aagtcgccgg gcgtgagcgt gcggccgatc tacatgctcg
     2161 atggcgagcc ggaggtgaac gaggtcttct tcgacaacgt cgaggtgccg gcggaaaacc
     2221 tgatcggcga ggagaacaag ggctggacct acgccaagca cctgctctcg cacgaacgca
     2281 ccaacatcgc ggacgtgaac cgcgccaagc gcgaactgga acgtctcaag cgcatcgcca
     2341 aggccgaggg catgtatgac ccggccgagg gcggtgacaa tgcgcggcgt ttccgtgagg
     2401 aaatcgcccg gctcgaagtg gacgtggtgg cactggaaat gctggtgctg cgcgtgctct
     2461 ccgccgagaa atcgggcaag aactcgctgg acatcgcggg cctgctcaag atcaaaggca
     2521 gtgaaatcca gcagcgctac agcgagctga tgatgctggc ggccgggcct tatgccctgc
     2581 ccctgatcca ggaagcgatg gaagcgggct ggcagggcga gtacgtcggc gccgcgcatt
     2641 gcgcgccgct ggcgtcgacc tacttcaact ttcgcaagac cacgatttac ggcggcagca
     2701 acgaggttca aaggaacatc gtggctcaga cggttcttgg ctgaagtgac ttcgtcatcc
     2761 cggacttgat ccgggatcca tggattgcgg gtcaagcccg caatgacaag tcacagccca
     2821 atgacagaaa caggagagca tatggatttc gatttcacca acgaccagga acagctgcgc
     2881 gacgcggtgc gcaaatgggt cgacaagggc tacgacttcg agcgccggcg cgcgatcgtg
     2941 aaggccggcg gcttcgacaa ggacgcatac agcgaactcg cgcaactcgg cctgacgggt
     3001 ctttatgtcc ccgaggacca gggcgggatg ggcatgggtc cggtcgaagg catggtggtg
     3061 atggaggaac tgggccgcgg catcgtgctg gagccgctgg cgcagaccct gatcgccggc
     3121 ggcatcctga acggctatgc ggacgatgcg gtcaagtcgg cctggctgcc caggattgcg
     3181 ggcggtgaag cgctggtggt gctggcccag caggagcgca aggcgcgcta tcggctcgac
     3241 atgtgcgaga ccagcgcaac ccgatcgggc ggcgcgtggg ccgtgacggg agccaagagc
     3301 atcgttgccg cgggcgatcg ggccgatgcg ttcctggtgc cggccacggt ggacggcaag
     3361 atcgcgctgt tcctggtcga acgcagcgcc gccggagtca ctgccaaggg ctacggcacg
     3421 caggaaggcg gccgcgccgc cgaattgggt ttcaagtccg caccggccac gctcgtgaca
     3481 ctcgacggcc tgacggcgct cgagcacgcg gtggacatcg gcattgccgc cagttgcgcg
     3541 gaagcggtag gcgttctgga caagaccatg gacgtcaccg tggaatacat gaacacgcgc
     3601 aagcaattcg gcgtgaccct gtccaccttc caggcgctgc gccaccgcgt ggccgacatg
     3661 aagatgcagc tggagctcgc gcgttcgatg agctactacg cgtcgctcaa gctcaatgcg
     3721 cccaccgagg agcggcgccg cgcgctcgcc cgtgcgaagt accagttggg cgtggctatg
     3781 cgcttcatcg gccagcaatc ggtgcagctg cacggcggca tcggcgtgac ggacgaatac
     3841 atcggcagcc actacttcaa gaagctcaca cagctggagc tgagtttcgg ggatacgctg
     3901 caccaacttg gcgaagtatc gaatcggatg caggacaccg cgggcgtgtt cgcctgaaag
     3961 tcagtcttcc ccgcggactg tcgtcccgaa gaggttgtca tcccgggctt gaccctaatg
     4021 gctgtcatcc cgggcttgac ccgggatcca cgttccttgg cgatcgcttg gccggcgtgg
     4081 catggattgc ggggccgggc ccgcaatgac aaatcacccg gcatccatga cttcctggat
     4141 tgcgggtcaa gcccgcaatg acacgcctgt atttgtcatg ccggactcga ttaaaacaat
     4201 tgtcatgccg gactcgatcc ggcatccatg actttgtgcc ttgtggcggg tcaggcccgc
     4261 aatgacaacg ctacgcgtgc agccgcgacg acctgggcag gtgcgccatc aggaactcca
     4321 tctggtcggc caggatgcgg cggttgcgca ggatgaagtc ttcccacagg ctggggatgt
     4381 agggcgcgta cagcagcggc atgcgcgcct gctcgggcgt gcggttgctc ttgcgatggt
     4441 tgcacgtgcg gcatgcggtc accacgttca tccagtggtc cctgccgttt tgagcgaacg
     4501 gaatgatgtg ttcgcgggtg aggtcttcct cgtggtagag gtgcccgcag taggcgcaga
     4561 cgttgcggtc gcgtgcgaac agcttgccgt tcgtgaggcc cggcctcagg tcgaaggggt
     4621 tgatgttggg cacgcccttg gtgccgatga tgctgttgac ggcgatctgg gactgcgcgc
     4681 cggtcatcgc gttgtggccg ccgcgaaaca atgccacctg cgcgcccacc tcccagcgca
     4741 cctcatcggc ggcatagtgc agcacggcct gctccagtga gatccacgac tggggcagtc
     4801 cttgggcgga cagcttcaga accttcaaaa cacgcctcca tcgacgaacg gcaaaaccag
     4861 gcagcgcaaa ggggcggcgc cctttggcgc tgtacagcac aatataccct gttttcgtgc
     4921 tcgatcgccc ccgcgaaaaa gcccgcggtg cttggcgggc cgggcgcccg acgctatcaa
     4981 aaagataccg acccagccgc aatgcagatt ttcagaggct tccaccatcc gggcatcgcc
     5041 cccgcctgcg cgctgaccat cggcaatttc gatggcgtgc accggggcca ccaggccatg
     5101 ctcgcgctgc tgaacaacga agcgcggcac cgcaacgtgc ccagctgcgt gctgaccttc
     5161 gagcctcatc cgcgcgatta ctttgcggcc atcgcgcgca agccggagct ggcgccggcc
     5221 cgcatcgcca cgctgcgcga caaactgacc gaactcgcgc gctgcggcgt ggaccagtgc
     5281 gtggtgcttt cgttcgacgc ccggctgtcg tcgcaaaccc ccgaggcttt catcgacaac
     5341 gtgctggtgc gcgggctggg cgtgcgctac gtgctggtcg gcgacgactt ccggttcggc
     5401 gccaagcggg ccggcgacta caccacgctc gatgcggccg gggcggcgcg ggggttcgac
     5461 gtggcccgca tgaacagcta tgaagtgcac ggcgtgcggg tttccagctc cgaggtccgc
     5521 gacgcgctgg gccgcggcga catggatggt gtcaccgcgc tgctggggcg gccctacagc
     5581 atcagcgggc acgtggtcca tggccgcaag ctggggcgcc agctgggatt caagacggtg
     5641 aacctgcgct tttcccactg gaagcccgcg gccggcggca tcttcgcggt gagggtgcac
     5701 ggcctggagg gcccggcgca gcccgatggc gccgacagct ccctgcccgg ggtggccaac
     5761 ctcggaatcc gcccctcgct gaaccccgac gacgtcaacg gcggccgggt gctgctggag
     5821 agccattgcc tggaatggcc gccgagcctg ggcgaggaag gggcctacgg taaaatcatc
     5881 cgcgtggaac tgctgcacaa attgcacgac gagctcaagt acgagagtct cgatgccctg
     5941 aaagccggca tcgcgaggga ctccgaccag gctcgtgcgt atttcgcgtc gactcgccgg
     6001 caaaccacgc gcgaccgaat ttgacgcggt ctgtcattgc gggccccgac ccgcaatcca
     6061 tctcccgggc cccgaatacc tggattccgg ctcaaggccg gaatgacaaa gtgacatccc
     6121 cgaggcatcg ccatgaccga caagaccgac taccgcgcca cgctcaacct gcccgacacg
     6181 ccgttcccga tgcgcgggga cctgcccaag cgcgagccgg gctgggtcaa ggagtgggaa
     6241 gacaagggcc tgtacaagaa gctgcgcgcc gcgcgctgcg aacacgagaa gttcgtgctg
     6301 cacgacggcc cgccctatgc caacggccag atccacatgg ggcacgcggt caacaagatc
     6361 ctgaaggaca tgatcgtcaa ggcgcggcag ctcaagggcc tggacgccgc ctacatcccg
     6421 gggtgggact gccacgggct gccgatcgag aacgcgatcg agaagaagta cgggcgtaac
     6481 cttccgcgcg acgagatgca ggcgaaaagc cgcgccttcg ccaccgagca gatcggcgtg
     6541 cagatggccg atttcaagcg gctgggggtg ctgggcgact gggaccatcc gtaccgcacc
     6601 atggaattca ttaacgaggc cgaggaaatc cgcgccttca agcgcgtgat cgagcgcggc
     6661 ttcgtctacc ggggcctgaa acccgtgtac tggtgcttcg attgcggctc gtcgctggcc
     6721 gaattcgaga tcgagtacgc cgacaagaaa tcgcagacgc tggatgtggg cttcgaggcg
     6781 gacgagccgc agaaggtgtt ggcggccttc gggctgccct caccccagcc ctctcccgca
     6841 agcgggagag ggagcgaacc ccaactctcc gctgggagag gaagcgaatc tcaactctcc
     6901 gctgggggag ggagcaatgc acacgatagc gccgacaggg attcgctccc tctcccctct
     6961 ggggagaggg caggggtgag gggcacaccc acccaaccca tctacgtcgt catctggacc
     7021 accacggcct ggaccatccc cgccaaccag gcgctcaacc tgaacccgga cctggagtac
     7081 tcgctggtgg aaaccgggcg gggcctgctg ctgctggcag cctcactggt cggcaggtgc
     7141 ctggagcgtt acgagctgac cggcaaggtg ctggccaccg tcaagggcga caagctggcg
     7201 ggcttgaact tccagcatcc gctccacgac gcggatcccg gctaccggcg cctctcgccc
     7261 gtgtacctgg ccgactatgc gacggcggag gacggcaccg gcatcgtgca ctcgtcaccg
     7321 gcttacggcc tggacgactt ccagtcgtgc gtggcccacg gcatggccta cgacgacatc
     7381 ctgaacccgg tccagggcaa cggctcctat gcgccggact tccccctctt cggcgggctg
     7441 aacatctgga aagccacgcc gcgcatcatc gaggcgctgc gcgcggccgg acgcctgttc
     7501 gccaccgaga acatcgccca tagctatccg cattgctggc gccacaagac gccggtgatc
     7561 taccgggcag cggcccagtg gttcatccgc atggacgaag gcgacggcgt gttcaccaag
     7621 gacaaggcgc ccaagacatt gcgccagatg gcgctggaag cgatcgagga aacgaacttc
     7681 ttcccggaga acggcaaggc gcggctgcgc gacatgatca ccgggcggcc cgactggtgc
     7741 atcagccgcc agcgcagctg gggcgtgccc ctgccctttt tcctgcacaa ggacacgggc
     7801 gagctgcatc cgcgcaccat ggaaatcctg gacaccgccg cggaaatggt cgagcaaggc
     7861 ggcatcgagg cctggagcaa ggccacggcc gaagccatcc tcggccccga ggacgcggcg
     7921 cactacacca agagcacgga catcctggag gtctggttcg actccggcac cacccatacc
     7981 acagtgctga agcactccca cgccggcgcc gggcatgaga gcgggccgga agccgacctg
     8041 tacctggaag gccatgacca gcatcgcggc tggttccatt cctcgctgct cacggcctgc
     8101 gcgatgtacg ggcgagcgcc ctaccgcggc ctgctcacgc acggcttcac ggtggacagc
     8161 caggggcgca agatgagcaa gtcgcagggc aacggcgtgg atccgcagga gaccaccaag
     8221 aagctgggcg cggagatcat ccgcctctgg gtcgcggcct cggactattc gggcgacatc
     8281 gccggcgacg acaagatcct ggcgcgcgtg gtcgatgcct accgccgcat ccgcaacacg
     8341 ctgcgcttcc tgctggccaa cgtgagcgat ttcgatccgg ccagggacgc cgtggcgctg
     8401 gaccagatgc tggagatcga ccgctatgcg atcactcgcg ccgcgcagtt ccaggccgag
     8461 gtactggccc actacgaggt atacgagttc catccggtgg tggccaagct gcaggtctat
     8521 tgctcggaag acctcggcgc tttctacctc gacatcctca aggaccggct ctacaccacg
     8581 gcgccgaaat cgctggcacg gcgcagcgcg cagacggcgc tgtggaacat cacgcacgcc
     8641 atgctgcgct ggatggcgcc gttcctcagc ttcacggccg aggaagcctg gaagctgttc
     8701 ggcgattccg aatcgatctt cttcgaaacc tatgccgaga tcggcggacc cgacaccgag
     8761 ctgctggata aatggacccg catccgggcc gcgcgcgagc tggtgaacaa ggagatcgaa
     8821 tcgctgcgtg aagccggcaa ggtcggctcg tcgctgcagg ccaacgtgca actgactgta
     8881 cccatggcca cccacggcga actgtggtcc gcgctggcca gcctcggaga cgacctgaaa
     8941 ttcgtcttca tcacctcggt catgcgggtg gtggcgggcg acgtgatggc cgtgaaggtc
     9001 accccgtccg aggcggtgaa gtgcgagcgc tgctggcact ggcgcaacga cgtcggccag
     9061 gat
//
LOCUS       DASWIY010000011         6938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1113423, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000011 DASWIY010000000
VERSION     DASWIY010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113423"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..819)
                     /locus_tag="VGA59_00500"
     CDS             complement(325..819)
                     /locus_tag="VGA59_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9718183.1"
                     /translation="MPTTRGAKDACDLLDADHKAVKKLFKEYDELTESKARGAVQKKK
                     DLAQQICHELTVHAQIEEEIFYPALREAIKEKDLIAEAAVEHQTAKDLISQIEGMAMR
                     EPDETFDAKVKVLGEYIEHHIKEEKNEIFPKARAARKLDLVAMRDELEARKEELMSET
                     HMMT"
     gene            1010..1996
                     /locus_tag="VGA59_00505"
     CDS             1010..1996
                     /locus_tag="VGA59_00505"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HEX9718184.1"
                     /translation="MLQLPTYDDVAAAARRLHGLAHVTPVLRSATADRLLKAQVFFKC
                     ENLQRMGAFKFRGAYNALAKFDPAQRKGGAIAFSSGNHAQAIALAARLLGIPATIVMP
                     LDAPQAKMDATRGYGGEVITYDRYTQDREALAAQLAAERGLTLVPPYDHPDVIAGQGT
                     AAKELFEQVGELDVLLVPLGGGGLLSGVALATRALSPQCKVYGVEPEAGNDGQQSLRK
                     GEIVHIATPNTLADGAQTQHLGRYTFEVIRRDVDDVLTAGDAQLVEAMRFFAERMKLV
                     VEPTGCLGFAAACHAGLPLEGLRVGVILSGGNVDLARYAQLIGAAGPGRGWA"
     gene            2024..3496
                     /locus_tag="VGA59_00510"
     CDS             2024..3496
                     /locus_tag="VGA59_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEX9718185.1"
                     /translation="MSFINLPLLAAAALLFLSLVLGIFSTRVGFPFLLVFLLAGALAG
                     EDGPGGFHFDDFGLSFWVGNIALAIILLDGGLRTSFATFRTGLKPAILLATVGVVATA
                     ALTGLAAMVLLDLDWRHGMLLGAIVGSTDAAAVFALLGRSGVKLNERVGATLEIESGM
                     NDPMAVYLTLAFIALALAPIPGGKAWMDMLSSFAAQFGWGAAIGIASGYAMAALVLKL
                     PDDGGGGGILALLVVAGGLGVFALAGLLGGSGFLAVYLFGLLVAYRAMSKLVSYLPAM
                     DGYAWLAQAGMFLLLGLLVTPSAMVKTALPAVGVAAVLMFVARPVSVWLCLAPFGFPA
                     REVWFISWVGLRGAVPIVLAVFPLMAGVPNAMLIFNVAFAVVLASLLLQGTTIRIAAH
                     VFRVALPDRSDERQLRALFGDFTLDAQAPIDSICSFYELPVPPTATAPVGQWLAENLR
                     REPVVGDALRLGPATLSVRAMEGSRITAVGLKLEPERRDA"
     gene            complement(3508..4191)
                     /locus_tag="VGA59_00515"
     CDS             complement(3508..4191)
                     /locus_tag="VGA59_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9718186.1"
                     /translation="MSRQTALATVSVRNLAKHYGEVPVFSHVSLEVAPGEFVAIVGES
                     GVGKSTLLNCMAGLDTWDEGSVLVGGEDLAALDDDSRALLRRHRIGFVFQAFHVLPHL
                     DVAQNVALPLMLLGEPDEGRVEWMLTAVGLAGLGARLPQQLSGGQLQRVAIARALVHR
                     PALLLADEPTGNLDPGTAAKVMDVLISQTREHGASLVLVTHSEAAAARADRVLLLTSE
                     GVAERTHAG"
     gene            complement(4188..5204)
                     /gene="miaA"
                     /locus_tag="VGA59_00520"
     CDS             complement(4188..5204)
                     /gene="miaA"
                     /locus_tag="VGA59_00520"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX9718187.1"
                     /translation="MAIRAGSEPRVLALAGPTASGKSTAALAIAQAHDAEIISVDSAL
                     VYRGMDIGTAKPSADERAAVPHHLIDIRDPLQAYSAAEFAADARRLVGEINARGRLAL
                     LVGGTMLYFKALFEGLDAMPAADAAVRAQLEDQAAREGWPALHAELARIDPPTAARLA
                     PNDSQRIQRALEVFRLSGQPLSSFHAGGNAGAADEWGSTPLFSLEPEDRGWLHARIAH
                     RFDAMLAAGFLDEVRALRSRGDLNSALPSMRCVGYRQAWEFLDAHEARGLDAHEAREL
                     GSQDAPPRPFPVAQLRDKAIFATRQLAKRQLTWLRGMPGRRVIACDAPDARARLLRQV
                     GGLL"
     gene            complement(5182..6414)
                     /locus_tag="VGA59_00525"
     CDS             complement(5182..6414)
                     /locus_tag="VGA59_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX9718188.1"
                     /translation="MPAAPQTVSNASSAMTPALIVLVLALLLGIQPITTDLYLPALPS
                     LAEGFGAPVSHAQLTLTTLLLAFGISQLAWGPLSDRFGRRPVLLWGLSAYTLASVGCA
                     LAASMPLLIVWRTVQGAAMGAAVMGARAIVRDLYTPEIGARVMSRGLTGLGVIACLSA
                     PLGGLLSDLWGWRYALTALAVFGALSLFVVALRFEETVPRKNPLALQPATLAATWLMI
                     LRHPTFLAWSALATASYAGLFTFLASSSFVFIKVLGLTRTQYGLVMFSMALAYILGTV
                     LCRRLLPRFGVRRSVAFAGVLTLTGGTLTGVLALAGLHNGWAIMLPFYLFMLAHGVHQ
                     PCGQSGAVGPFPQAAGAASALNGFLMMLAAFGVGGWIGTRLDGTVFALTNGIWFWSVC
                     IAAMAWTVVQRYGDPRRF"
     gene            complement(6450..>6938)
                     /locus_tag="VGA59_00530"
     CDS             complement(6450..>6938)
                     /locus_tag="VGA59_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9718189.1"
                     /translation="FGKSTPGLPSEEMAYEDARAAWDWLAAKHPQRPRYIFGHSLGGA
                     IAIDLAAQVRDEQGTIVEGTFTSIPDVASSMRWGWLPVGLLITQRFEAVKKVAQIGSP
                     LLVVHGENDNLINKELGRKLYEAAQGRKRFLLVEGGSHFSTMAIGAAKYREALADLFS
                     LR"
BASE COUNT         1117 a         2304 c         2318 g         1199 t
ORIGIN      
        1 ggcgcgcacc gcgctggaga actcgctcag ggccgaggcg ttgatcaaat gaaccgaaaa
       61 cgccagtgcc acgcccagca tcaccgccag aaccgcggcc gcattgcgcc acggatggtg
      121 gcgcagttcc tgcaaggaga aagtgcgaag cagggcgagc atgcgcccat tgtgcagccc
      181 cgagtgatag cggggatgac agatcctctg tcattgcggc cttgagccgc aatccacaaa
      241 gtcatggatc ccgggcgctg aaaccccgga cttgatccgg ggccgggatg acaaagtcgt
      301 cgattactcc ttggccgcag gccgttacgt catcatgtgc gtctcggaca tcagctcttc
      361 cttgcgtgcc tccagttcgt cgcgcatggc caccaggtcg agcttgcggg ctgcacgtgc
      421 ctttggaaag atttcgttct tctcttcctt gatgtgatgc tcgatgtatt cacccagcac
      481 cttgaccttg gcgtcgaacg tctcgtcggg ttcgcgcatg gccatgccct cgatctgcga
      541 gatcaggtcc ttggcggtct ggtgctcgac ggcggcttcc gcgatcaggt ccttttcctt
      601 gatcgcctcg cgtagggccg ggtagaagat ctcttcctcg atctgggcat gcaccgtcag
      661 ctcgtggcag atctgctgcg ccagatcctt ctttttctga accgcgccgc gcgccttcga
      721 ctcggtcagt tcgtcgtatt ccttgaacag tttcttgact gccttgtggt cggcatccag
      781 aagatcgcaa gcgtcctttg ctccgcgggt agttggcatt tcgttgctcc ttgggttgaa
      841 gacccccgat catccggagc gctgaggctg ctcgctgtag gagaacgtcg gcattgcctg
      901 aagaacgcca ccgacgcgcc cgcaggcaca tggttcgggg ctgcgcagcg gctatgcttg
      961 agcctgttca attcgtactc gcaccagctg tgtgaacagg tcctacgcta tgctccagct
     1021 tcccacctat gacgacgttg ccgccgccgc gcggcgcctg catggcttgg cccatgtcac
     1081 gccggtgctg cgttcggcca cggccgaccg gctgctcaag gcccaggtgt tcttcaagtg
     1141 cgagaacctc cagcgcatgg gcgccttcaa gttccgcggt gcctacaacg cgctcgcgaa
     1201 attcgacccg gcccagcgca agggcggcgc gatcgccttt tcctcgggca atcacgcaca
     1261 ggcgatcgcg ctcgcggccc gcctgctggg cataccggcc accatcgtga tgccgctcga
     1321 tgcgccccag gccaagatgg acgccacccg cggctatggc ggcgaggtga tcacctacga
     1381 ccgctacacg caggaccgcg aggcgctcgc ggcccaactc gcggccgagc gcggcctgac
     1441 gctcgtcccg ccttatgacc accccgatgt catcgccggc cagggcacgg cagccaagga
     1501 gttgttcgag caggtgggcg aactcgatgt tctgctggtg ccgctgggcg gcggcggcct
     1561 gctcagcgga gtggcgctgg caacgcgcgc gctgtcgccg cagtgcaagg tctacggcgt
     1621 cgagccggag gctggaaacg acggccagca atccctgcgc aagggcgaga tcgtccacat
     1681 cgccacgccc aacaccctgg ccgacggtgc gcagacgcag catctgggcc gctacacgtt
     1741 tgaagtgatc cgtcgcgacg tcgacgacgt tctcacggcc ggcgacgcgc agctggtcga
     1801 agccatgcgt ttctttgcgg agcgcatgaa gctggtggtg gagcccaccg gttgcctcgg
     1861 cttcgccgcg gcctgccacg cgggcctgcc cctggaaggc ctgcgagtgg gcgtgattct
     1921 cagcggtggc aacgtggacc tggcgcgtta cgcgcaactg atcggcgcag cgggtccggg
     1981 ccggggctgg gcataggcag ttcgtgggtt cgcaggcagc aggatgagct tcatcaatct
     2041 cccactactt gctgccgcgg cactgctgtt tctcagcctt gtccttggca tattctccac
     2101 gcgtgtcggc tttcctttcc tccttgtgtt tctcctcgcc ggcgcgctgg ccggggagga
     2161 cgggccaggt ggtttccatt tcgatgactt cggcctcagc ttctgggtgg gaaacatcgc
     2221 cttggcgatc atcctgctgg acggaggact gcgcacgtcc ttcgccacgt tcagaaccgg
     2281 cctgaagccc gccattcttc tggcgacggt cggcgtggtg gcgactgccg ccctgacggg
     2341 tctggctgcg atggttctgc tggaccttga ctggcggcac ggcatgctac tgggtgccat
     2401 cgtaggatcg accgacgcag cggccgtgtt tgccctgctc ggtcggtccg gcgtgaagct
     2461 gaacgaacgc gtgggagcaa cgttggagat cgagtcaggg atgaacgacc cgatggcggt
     2521 ctacctgacg ctggccttca ttgcccttgc attggcgccg attccaggcg gcaaggcatg
     2581 gatggacatg ctgtcgtctt ttgccgctca gttcggctgg ggcgctgcga tcggtattgc
     2641 gagtggatat gccatggccg cccttgtgct caagcttccg gacgacggcg gtggcggcgg
     2701 catccttgcg ctgctggtgg tcgccggcgg actgggcgtg tttgcgctcg ccggcctgct
     2761 cggcggatcc gggtttctgg cggtgtattt gtttggcctc ctggtcgcat atcgcgccat
     2821 gtccaaactc gtttcctatt tgccggctat ggacggctac gcatggctgg ctcaggcggg
     2881 catgttcctg ctgctgggcc tgctagtgac gccctcggca atggtgaaga cggcgctgcc
     2941 tgccgttggg gtcgctgcgg tcctgatgtt cgtcgctcgc cctgtcagcg tctggctctg
     3001 cctggctccc tttggattcc cggcacgcga ggtgtggttc atctcctggg tgggtctgcg
     3061 cggggctgtt ccgatcgtgc tggcggtatt cccattgatg gcgggagtcc ccaatgcgat
     3121 gctcattttc aacgtggcat tcgccgttgt gttggcgtct ctgctactgc aaggcaccac
     3181 catcaggatc gcggcccacg tcttcagggt ggctttgccc gaccgaagtg acgagcggca
     3241 gctgagagct ctgttcggtg acttcacact cgatgcgcag gccccgatcg attcgatttg
     3301 cagcttctac gagttgccgg ttcctccgac ggccactgcg cctgtcgggc agtggctggc
     3361 ggagaacttg cggcgcgaac ccgtcgttgg agatgccctt cgccttggac ctgcgacgtt
     3421 gagtgttcgg gcaatggaag gatcgagaat cacggctgtc gggctgaagc tcgaacccga
     3481 gcgacgtgac gcatgaggca atgccgctca ccctgcgtga gtccgctcgg ccacgccctc
     3541 gctcgtgagc agcagcaccc ggtcggcacg cgcggcagcg gcctcggaat gcgtgaccag
     3601 caccagcgac gcgccatgtt cgcgcgtctg cgagatcagc acatccatca ccttcgccgc
     3661 ggtgccggga tcgaggttgc cggtgggttc gtccgccagc aacagggccg ggcgatgtac
     3721 gagagcccgg gcgatcgcca cgcgttgcag ctggccgccg gaaagctgct ggggcaggcg
     3781 cgcccccagc ccggccaggc cgacggccgt cagcatccat tcgacgcggc cctcgtccgg
     3841 ctcgcccagc agcatcagcg gcagcgccac gttctgcgcc acgtccaggt gcggcagcac
     3901 gtggaaggcc tggaacacga aaccgatccg gtggcggcgc agcagggcgc ggctgtcgtc
     3961 atcgagcgcc gccaggtcct cgccgcccac gagcaccgag ccctcgtccc aggtgtcaag
     4021 gccggccatg cagttgagca gggtggactt gcccacaccc gattcgccga cgatggcgac
     4081 gaattcgccg ggggcgacct cgagcgatac atgcgagaac accgggacct cgccatagtg
     4141 cttggccagg ttgcgcaccg acaccgtggc gagagcggtc tgtcggctca taggagaccg
     4201 ccgacctgcc gcagcagtcg agcccgtgca tcgggcgcgt cgcaggcgat cacgcggcgg
     4261 ccgggcatgc cgcgcagcca ggtcaattgc cgtttcgcga gctggcgtgt ggcgaagatg
     4321 gctttgtcgc gcaactgtgc gacggggaac ggccgaggcg gcgcatcttg cgagccgagc
     4381 tcgcgcgctt cgtgcgcgtc cagcccgcgc gcctcgtgcg cgtccagaaa ttcccaggct
     4441 tgccgatagc ccacgcagcg catgctgggc aacgcggaat tcaggtcgcc gcgcgagcgt
     4501 aacgccctga cctcatcgag gaaacccgcc gcgagcatcg catcgaagcg atgggcgatg
     4561 cgcgcgtgca gccagccgcg atcctcgggt tccagtgaaa acaaaggcgt tgagccccac
     4621 tcgtccgcag cgcccgcatt cccgcccgca tgaaaactgg acagcggctg ccccgacaac
     4681 cggaacactt ccagcgcgcg ctggatgcgt tgcgagtcgt tgggcgccag ccgggccgcg
     4741 gtgggcgggt cgatgcgcgc cagctcggcg tgcagcgcgg gccagccttc ccgggcggcc
     4801 tggtcttcaa gctgcgcgcg cacagcggcg tcggccgccg gcatcgcgtc cagcccctcg
     4861 aacaatgcct tgaaatacag catggtgccg cccaccagca gcgccagcct gccccgcgca
     4921 ttgatctcgc cgaccagccg ccgggcgtcg gcggcgaact cggcggcgct gtaggcttgc
     4981 aggggatcgc ggatgtcgat caggtggtgg ggcaccgcgg cgcgctcgtc cgccgagggc
     5041 ttggccgtgc cgatgtccat gccgcggtac accagcgccg agtcgacact gatgatctcg
     5101 gcatcgtgtg cctgcgcgat ggcgagggcc gcagtgctct tgccggaggc cgtgggcccg
     5161 gccagcgcaa gcaccctggg ctcagaacct gcgcggatcg ccataccttt gcacgacggt
     5221 ccacgccatg gcggcgatgc acacgctcca gaaccagatc ccgttggtga gcgcgaagac
     5281 ggtgccgtcc aggcgagtgc cgatccagcc gcccaccccg aaagcggcaa gcatcatcag
     5341 aaagccgttg agtgccgacg ccgcccccgc cgcctgaggg aacggtccga cggcgccgct
     5401 ctgcccacag ggctggtgca cgccgtgcgc cagcatgaac aggtagaagg gcagcatgat
     5461 cgcccagccg ttgtgcaggc ccgccagtgc gagtacgccc gtcagtgttc cgccggtgag
     5521 cgtgagcacg cccgcgaaag ccacggagcg ccgcacgccg aagcgcggca gcaggcgacg
     5581 gcacaggaca gtgcccagaa tgtaggcaag cgccatcgag aacatcacca ggccgtattg
     5641 cgtgcgcgtc aggcccagca ccttgatgaa cacgaacgag gacgacgcga ggaaggtgaa
     5701 cagccccgcg tacgaagccg tggccagcgc cgaccaggcg aggaacgtcg gatggcgaag
     5761 aatcatgagc caggtcgccg ccagggttgc cggctgcagc gccagcgggt tcttgcgcgg
     5821 caccgtctcc tcgaagcgca gggccaccac gaacagcgac agcgcgccga aaacggcgag
     5881 ggccgtcagc gcgtagcgcc acccccagag gtcggacagc aggccgccca gcggtgcgga
     5941 caggcaggcg atcacgccca ggcccgtcag gcccctggac atcacgcgcg caccgatctc
     6001 cggcgtatac aggtcgcgca cgatggcacg ggcgcccatc accgccgcgc ccatggccgc
     6061 gccttgcacg gtgcgccaga cgatcagcag cggcatcgaa gcggccagcg cgcagccgac
     6121 ggaagccagg gtataggcgc tcaggcccca cagcaacacg ggccggcgcc cgaaccggtc
     6181 cgacagcgga ccccatgcca gctgcgagat gccgaatgcc agcaacaagg tcgtcagcgt
     6241 gagttgcgcg tgcgacacgg gggcgccgaa accttcggcg agcgacggca gcgcgggcag
     6301 gtacaggtcg gtggtaatgg gctggatgcc gagcagcagg gccagcacca gcacgatcag
     6361 cgccggcgtc atggcggagc tggcgttgga gacggtttgc ggcgcggcgg gcatccggcc
     6421 gagggtagca gaagccttag ggcgaagcct tatcgcaggc tgaacaaatc cgccagcgcc
     6481 tcccggtact tcgccgcgcc gatcgccatc gtgctgaagt gcgagcctcc ctccaccagc
     6541 aggaaacgct tgcggccctg cgcggcctcg tacaacttgc ggccgagctc tttattgatc
     6601 aggttgtcgt tctcgccgtg gacgaccagc agcggcgagc cgatctgcgc gactttcttg
     6661 acggcctcga agcgctgcgt gatcagcagg cccaccggca gccagcccca cctcatgctg
     6721 ctggccacgt cgggaatgga ggtgaacgtg ccttcgacaa tggtgccctg ttcgtcgcgc
     6781 acctgcgcgg ccaggtcgat ggcgatggcg ccacccagcg aatggccgaa gatgtagcgc
     6841 ggccgctgcg gatgcttcgc tgccagccag tcccaggcgg cgcgggcgtc ctcgtacgcc
     6901 atctcttccg aagggagccc gggtgtgctc ttgccgaa
//
LOCUS       DASWIY010000012        14018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1114491, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000012 DASWIY010000000
VERSION     DASWIY010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 14018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14018
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114491"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1061)
                     /locus_tag="VGA59_00535"
     CDS             complement(69..1061)
                     /locus_tag="VGA59_00535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEX9718190.1"
                     /translation="MAAIPQLLSLRVAEARPLNSLIRLLRLVAPDGSALPGFTAGAHL
                     RVQVTLPEGKTDWRHYSLIDFRAEPGATEAPREYVIAVRREDEGRGGSRFMHERVQPG
                     DVLTIETPKNEFALRPLDSRAVLIAGGIGITPLASMAAQRKAEGRAVRLHYAGRSRTL
                     MAFLPELQQLLGDDLKLHADDEAGAPLDVDALLAGCSADDQLYVCGPKVLLDAVLANT
                     QARGWTHERVHFELFTTPVAEAGDHSFEVALAQSGKTFTVPAGQTILDCLIEHGCDPL
                     FDCKRGECGVCSTAVIEGDIDHRDYFLSDAEKASGKVMQICISRARGARLVLDL"
     gene            complement(1061..2581)
                     /locus_tag="VGA59_00540"
     CDS             complement(1061..2581)
                     /locus_tag="VGA59_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HEX9718191.1"
                     /translation="MSAQPIKIAILAMGGEGGGVLADWIVQLGEANGYIAQTTSVPGV
                     AQRTGATIYYVELYPRAQAERDGGKPVLALMPLPGDVDVVLASELMEAGRGVQRGLVT
                     PDRTTLIASTHRVYSITEKTAMGDGRADPRSLLAHGESAAKRFVHFDMEQVAEENGSV
                     ISAVLFGALAGAGVLPFSRARFEATVERGGVGVKSSLRAFAAGFERAAQPIAAVPEAA
                     ATGDEPAPARQAPRDPQVRALLARVYDDFPPAARTILIEGLRRLIDYQDSAYAALYLD
                     RLARQGSRPAVDEGLLAETGRYLALWMSYEDTIRVADLKTRASRFDRVVAEVKVQGDQ
                     LLAINEFMHPRLEEICDTLPAALGRWLASSGAPRRLVERFTRKGRVVATSSLGGFLLL
                     YLVAGLKRWRRGTLRFAVENARIEQWLARIEATAKFNPQLALEIAQCQRLVKGYGDTH
                     ERGWANFAILMAAADQAGATMAPATLRELREAALADEHGDKLRALLARQPVVKEAA"
     gene            complement(2586..4733)
                     /locus_tag="VGA59_00545"
     CDS             complement(2586..4733)
                     /locus_tag="VGA59_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX9718192.1"
                     /translation="MAERSFVEEVKKLRLGTGEVFRGEGILAVTKALLESGVAYVAGY
                     QGAPISHLMDVLADAQDILGEYGIRFENSASEATAAATLAASVNYPLRGAVTFKSTVG
                     TNVASDALANLASGGVTGGALIVVGEDYGEGSSIMQERSHAFAMKSQIWLLDPRPNLP
                     SIVGAVKHGFALSEASNTPVMLQLRLRACHVHGEFVAADNKAPTFTLKDAMENPQRDV
                     SRIVLPPASFVHEQEKVRERWPAALRYIEQHGLNEFFGEGTSDIGIIVQGGSYNTLVR
                     VLERLGMADVYGNSRIPLYVMNVAYPVVDSEVLRFCEGKRAVLMVEEGQPNFIEQNIA
                     LVLRQAGLTAALHGKDLLPMAGEYTAAESLKGVRAFLERYGELLPVEPLPRAAQAPKV
                     IPLKALNDTVQGRPPGFCTGCPERPIFTAMKLVERELGPHHVSADIGCHLFSILPPFN
                     LGNTTMGYGLGAAGAAALNVPAGKRAVSVMGDGGFWHNGLTSGIANAVFNRSDNLTIV
                     VDNSYTSATGGQDIMSSTAKNPTRSTGHAIEAAVRGVGVKWVRTMRNTYDVAGMRDAL
                     RDALTSKTKGPKVLIAQSECMLNKQRREKPLQRKSVAEGKRVVRDRFGIDSDTCTGDH
                     SCIRLSGCPSLSIKPNPDPLRTDPVATVLDSCVGCGLCGEVSHAAVLCPSFYKAQIVS
                     NPSRWDRLRQRLRGAVIGRLQRREARARERFAF"
     gene            4872..5354
                     /locus_tag="VGA59_00550"
     CDS             4872..5354
                     /locus_tag="VGA59_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9718193.1"
                     /translation="MGKQDMATAAHFVDDYLPALLAQATQLISGEFHRVVKARGVTVS
                     EWRVLATLAGGEPLSIGRLAQITVTKQPTLTRVLDRMEARGEVRRIAHAEDRRVTLVG
                     ITPAGSRLVTGLIKLAREHERRVLEPFGLQRAADLKTTLRRMIELHKGLANDDAGDEE
                     "
     gene            complement(5373..6359)
                     /locus_tag="VGA59_00555"
     CDS             complement(5373..6359)
                     /locus_tag="VGA59_00555"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX9718194.1"
                     /translation="MKNFVAVGAGRMGRGIAIAFAYAGHRIALVDLKPRTREAWQKLC
                     DDARAEIAGSLHSLAQLGAMRADQIGAIAARVEFVDAAAAPQALAAADLVFEGVPETM
                     EAKRDAFEHLNRHCRGDAVLTSTTSSILVTQLAGLVRRPERFFNMHWLNPAYLIPVVE
                     LSTHPKTNAAAVARTREVMESIGKLPVVCGVAPGYIVPRLQALIMNEAARMVEEGVAS
                     PEEIDKATRFGLGLRFAALGVVEFIDFGGADILHHASREMSGSIDAARYTAPGVIGKM
                     VADNRLGLKTGGGFYDYSGRDVAAYRLDVLARTLGMLRHAGLWRPPAEDTLP"
     gene            complement(6364..7437)
                     /locus_tag="VGA59_00560"
     CDS             complement(6364..7437)
                     /locus_tag="VGA59_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HEX9718195.1"
                     /translation="MNIAILGGGHGCYAAAADLSEQGHQIRLWRRDAAALQSVVDSGG
                     IVLKDSAGAREVGIALATADIGQALKGAQLILIPSPATAQADIARQMAPHLSDGQVVF
                     LAPGSFGSYVMARLVRGAGSRSDVAWAETGTLPYLARKHGEREVNVTIRAVRLPTGVY
                     PERGAQHALTVIRQAYPSVHGCGDALSGALMNAGPIIHPPLIVMNAAPLQHFERWDIH
                     NEGTQPAVRAVTDRLDAERIAVRDALGYGAPHYPLADHYDNDRWMYGDAHKKLVKSGD
                     WREHIDLRTHRYITEDSELGLAFLASAARYAGVDAPLAHGLLAVVGGFLGRDLRRGPR
                     SFEALGLAQLSPQDLRQRLHDGE"
     gene            complement(7524..8582)
                     /locus_tag="VGA59_00565"
     CDS             complement(7524..8582)
                     /locus_tag="VGA59_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9718196.1"
                     /translation="MRRSRQDPTRESQENRFVKLDIFNHIFPRAYYERMLEVAPNGKD
                     MHKRVREIPCIVDLDERFRIMDSFGDYQQVICLGSPPIEVFGPPPLSTEMARLANDGM
                     AALVDKHPERFPAFIASLPMNDPQGLLRETERAIAQLGAVGVQVFTNVLGKPLTAPET
                     RPLFDLMAKLDRPIWLHPARGADFPDYKGEKKSHYEIWWTFGWPYETSAAMAHMVFEG
                     LFDAHPGLKVITHHMGGMIPYFEGRVGPGWDQLGSRTSDEDYTLVLKKLKKRPLDYFR
                     MFYADTALFGARAATEMGLKFFGVDHVVFSSDAPFDPEKGSAYTKWTIEIIDSLDITP
                     AQRHALYEGNARKLLKLG"
     gene            complement(8736..9725)
                     /gene="dusA"
                     /locus_tag="VGA59_00570"
     CDS             complement(8736..9725)
                     /gene="dusA"
                     /locus_tag="VGA59_00570"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEX9718197.1"
                     /translation="MDESVSRWRMSVAPMMDWTDRHCRYFHRLLSRHALLYTEMMTTG
                     ALIHGDVPRHLDFNPEEHPVALQLGGSDAAELAHCARLGEQWGYDEINLNCGCPSERV
                     QRGSFGACLMAEPKLVADCVKAMVDATRVPVTVKHRIGIDKSENYEFMRDFVGTVSEA
                     GCEVFLVHARNAWLKGLSPKENREVPPLRYEMVHRLKHDFPHLTIAVNGGITAAEQVA
                     GQLQRVDGVMIGREAYHNPWWIAQWDAQFFGESETGERTRESVETRMTEYMAREAAAR
                     GTPWSAIARHMLGLRNGLPGARRWRQTWSDHGLKDVHPRDVMALAHDAVARAA"
     gene            9943..10182
                     /locus_tag="VGA59_00575"
     CDS             9943..10182
                     /locus_tag="VGA59_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718198.1"
                     /translation="MATSNSSIGGDDGFELTSPGQRTGEGAGSVLPYLHDSLATKPGE
                     LFPAEHPKPAKSLAGRMRKALLDIRKPKKNQRGPR"
     gene            10474..10881
                     /locus_tag="VGA59_00580"
     CDS             10474..10881
                     /locus_tag="VGA59_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9718199.1"
                     /translation="MKHFDLEPSMLTLRGVFYPTGYMFIMFPTEKDARDAERALEEHG
                     YTGESSSLLTPEDIQQKIARTVGNADIPLPSAGTEADTVRHFTELAGKGHYALMIHAP
                     TAAETERIMELLKDFPISYGQKYRHLVIEDLVE"
     gene            complement(11109..11846)
                     /gene="phbB"
                     /locus_tag="VGA59_00585"
     CDS             complement(11109..11846)
                     /gene="phbB"
                     /locus_tag="VGA59_00585"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEX9718200.1"
                     /translation="MSQKIAYVTGGMGGIGTAICQRLHKEGFKVIAGCGPTRDFGKWL
                     GEQQAQGFTFYASVGNVGEWTSTVDAFSKAKAEHGSIDVLVNNAGITRDRMFLKMTRE
                     DWDAVIETNLNSMFNVTKQVVGDMVEKGWGRIVNISSVNGEKGQAGQTNYSAAKAGMH
                     GFTMALAQELATKGVTVNTVSPGYIGTDMVKAIRADVLEKIVATIPVKRLGEPSEIAS
                     IIAWLASEDGGYSTGADFSVNGGLHMG"
     gene            complement(11924..13105)
                     /locus_tag="VGA59_00590"
     CDS             complement(11924..13105)
                     /locus_tag="VGA59_00590"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9718201.1"
                     /translation="MEDIVIVAAARTGVGKFGGSLARIPAAELGAIVIKEAIARAKLD
                     PAQVGEVIMGQVLAAGAGQNPARQALIKAGIPKEVPGMTINAVCGSGLKAVMLAAQAI
                     AWGDSEIVVAGGQENMSAAPHLLMGSRDGQRMGDWKMIDSMIVDGLWDVYNQYHMGVT
                     AENVAKQYGIDRAAQDALALASQQKATAAQDAGKFKDEIVPVSIPQRKGDPLVFDTDE
                     YVNRKTNAEALAGLRAAFDKTGTVTAGNASGINDGAAAVVVMSAKKAAALGLKPLARI
                     VAFGTSGLDPATMGMGPVPASRKALQRAGWNVQDVNLFELNEAFAAQACAVNKELAID
                     PAKVNVNGGAIAIGHPIGASGCRILVTLLHEMQRREAKKGLAALCIGGGMGVSLAVER
                     P"
     gene            complement(13247..>14018)
                     /locus_tag="VGA59_00595"
     CDS             complement(13247..>14018)
                     /locus_tag="VGA59_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9718202.1"
                     /translation="LAVLAARGEEPLASATLLTSFLDFSDTGVLDLFIDEAFVKFRET
                     HLGRGGLLKGNELASTFSFLRPNDLVWNYVVGNYLKGETPPPFDLLYWNSDSTNLPGP
                     MYAWYLRNTYFENNLVKPGKCTVCGEKVDLRQVDLPVYIYGSREDHIVPIAGAYASTK
                     HLPGKRRFVMGASGHIAGVINPPSKNKRSYWTNDKLPKSHEEWLAGATEHPGSWWTDW
                     SRWLKGYAGKLIAAPKSYGRGKHKAIEPAPGRYVKARA"
BASE COUNT         2282 a         4714 c         4560 g         2462 t
ORIGIN      
        1 tggctgaacc agggcccgca ctgcctcagg gttgccgcgg taggtggtct tcatggtcgg
       61 aatcctgttc acaggtcgag caccagccgc gcgccacggg cgcgcgagat gcagatctgc
      121 atcaccttgc ccgaagcctt ctcggcgtcg ctcaggaaat agtcgcggtg atcgatatcg
      181 ccctcgatca ccgccgtgga acaaacgccg cattcgccgc gcttgcagtc gaacagcggg
      241 tcgcagccgt gctcgatcag gcagtccagg atggtctggc ccgcgggcac ggtgaaggtc
      301 ttgccgcttt gcgccagcgc cacctcgaac gagtggtcac cggcctcggc cacgggcgtg
      361 gtgaacagct cgaaatgcac gcgctcgtgg gtccagcccc gcgcctgcgt gttggccagc
      421 acagcgtcga gcagcacctt ggggccacag acatagagct gatcgtcggc ggagcagccg
      481 gccagcagcg cgtccacgtc cagcggcgcg ccggcttcgt cgtcggcgtg cagtttcagg
      541 tcgtcgccaa gcagctgctg cagttccggc aggaaggcca tcagcgtgcg gctgcggccg
      601 gcgtagtgca gccgcaccgc gcggccttcg gccttgcgct gcgccgccat gctcgccagc
      661 ggcgtgatgc cgatgccgcc ggcgatcagc accgcgcggc tgtccagcgg gcgcagcgca
      721 aattcgttct tcggcgtctc gatcgtgagg acgtcgcccg gttgtacgcg ctcgtgcatg
      781 aagcgcgagc cgccgcggcc ttcgtcctcg cggcgcaccg cgatcacgta ctcgcgcggc
      841 gcttcggtgg caccgggctc ggcccggaag tcgatcagcg aatagtggcg ccagtcggtc
      901 ttgccctccg gcagcgtcac ctgcacgcgc aggtgggcgc cggcggtgaa ccccggcagc
      961 gcgctgccgt ccggtgccac cagccgcaac agccggatca gcgaattcag cgggcgggcc
     1021 tcggccaccc gcagcgacag cagttggggg atcgcggcca tcacgcggcc tccttcacca
     1081 cgggctggcg cgccagcagc gcgcgcagct tgtcgccgtg ctcgtcggcc agcgctgcct
     1141 cgcgcaactc acgcagcgtc gcgggagcca tcgtcgcgcc ggcctggtcg gcggctgcca
     1201 tcaggatcgc gaaattggcc caaccgcgct cgtgcgtgtc gccataaccc ttgaccaggc
     1261 gctggcactg cgcgatttcc aatgcaagct gcggattgaa ctttgccgtg gcctcgatgc
     1321 gggcaagcca ctgctcgata cgcgcgttct cgaccgcaaa gcgcaaggta ccacgccgcc
     1381 agcgcttgag gccggccacc aggtacagca gcaggaagcc gcccagagag ctggtcgcca
     1441 ccacccggcc cttgcgcgtg aagcgctcga ccaggcgccg gggcgcgccc gagctcgcca
     1501 gccagcggcc cagcgcggcc ggcagcgtgt cgcagatttc ctccagccgc ggatgcatga
     1561 attcgttgat cgccagcagc tgatcgccct ggaccttcac ttccgcgacc acgcggtcga
     1621 aacggctggc gcgcgtcttc aggtcggcca cgcggatggt gtcctcgtag gacatccata
     1681 gcgccaggta acgcccggtc tcggcaagca agccctcgtc cactgcgggc cggctccctt
     1741 gccgcgcgag ccgatcgagg tacagcgcgg cgtaggccga gtcctggtag tcgatgaggc
     1801 ggcgcaagcc ttcgatgagg atggtgcgcg cagccggcgg aaagtcgtcg tacacgcgcg
     1861 ccagcaacgc gcgcacttgc gggtcgcgcg gcgcttgccg cgccggcgca ggttcatcgc
     1921 ccgtggcggc ggcttccggc acggcggcga tgggctgggc agcgcgctcg aagccggccg
     1981 cgaaggcccg caggctggac ttcacgccga cgccgccgcg ttccaccgtc gcctcgaacc
     2041 gggcccggct gaaaggcagc acgccggcgc cggccagcgc gccgaacagc acggcgctga
     2101 tcacgctgcc gttctcttcg gcgacctgct ccatatcgaa atgcacgaag cgcttggctg
     2161 cggactcgcc gtgggccagc aggctcctgg ggtcggcgcg gccgtcaccc atcgccgtct
     2221 tttcggtgat ggagtacacg cggtgggtcg atgcgatcag cgtcgtgcgg tcgggcgtta
     2281 ccagtccgcg ttgcaccccg cggccggctt ccatcaattc ggaggccagc accacgtcga
     2341 cgtcgcccgg cagcggcatc agcgccagca ccggcttgcc gccgtcgcgc tcggcctggg
     2401 cgcgcgggta aagctccacg taatagatgg tcgcgccggt gcgctgcgcg acgcccggca
     2461 ccgacgtggt ttgcgcgatg tagccgttgg cctcgccgag ttggacgatc cagtcggcca
     2521 gcacgccgcc gccctcgccg cccatggcga gaatcgcgat cttgatgggt tgcgcgctca
     2581 tctgctcaga atgcgaaacg ctcgcgcgcg cgcgcttccc ggcgctgcag ccggccgatc
     2641 actgcgccgc gcaggcgctg gcgcagccgg tcccagcggc tcgggttgct gacaatctgc
     2701 gccttgtaga acgaggggca cagcacggcg gcatgcgaga cctcgccgca caggccacag
     2761 ccgacgcagg agtcgagcac ggtggcgacc gggtcggtgc gcagcgggtc cggattgggc
     2821 ttgatcgaca gcgacgggca gccggacagg cgaatgcacg aatggtcacc ggtgcaggtg
     2881 tcggaatcga tgccgaagcg atcgcgcacc acgcgcttgc cctccgccac cgactttcgt
     2941 tgcagcggct tctcgcgccg ctgcttgttc agcatgcatt cggactgcgc gatcagcacc
     3001 ttggggccct tggtcttgct ggtcagtgcg tcacgcaacg catcgcgcat gcccgccacg
     3061 tcgtaggtgt tgcgcatagt ccgcacccac ttgacgccga cgccgcgcac ggcggcttcg
     3121 atggcgtggc cggtgctgcg ggtcgggttc ttcgcggtgg acgacatgat gtcctgaccg
     3181 ccggtggcgg aggtgtagct gttgtcgacc acgatggtga ggttgtcgct gcggttgaac
     3241 accgcgttgg cgatgccgct ggtcaagccg ttgtgccaga acccgccgtc ccccatcacg
     3301 ctgaccgccc gcttgcccgc cggcacgttg agcgcggcgg cgccggctgc gcccaggcca
     3361 tagcccatcg tggtgttgcc gaggttgaac ggcggcagga tggaaaacag gtggcagccg
     3421 atgtcggcgc tgacatggtg tggcccgagt tcccgctcga ccagcttcat cgcggtgaag
     3481 atcggccgtt cggggcagcc ggtgcaaaag cccggcgggc ggccctgcac ggtgtcgttc
     3541 agcgctttca acggaatgac tttgggcgcc tgcgcggcac gcggcagcgg ctcgacgggc
     3601 agcagctcgc cgtagcgttc gaggaaggcg cgcacgccct tcaacgactc ggccgccgtg
     3661 tattcgccgg ccatgggcaa caggtccttg ccgtgcagcg cggcagtcag gcccgcctgc
     3721 cgcagcacca gcgcgatgtt ctgttcgatg aagttgggct ggccctcctc caccatcagc
     3781 accgcgcgct tgccctcgca gaagcgcagc acttccgaat cgaccaccgg ataggccacg
     3841 ttcatcacat acagcgggat gcggctgttg ccgtacacgt ccgccatgcc cagccgctcc
     3901 agtacgcgca ccagcgtgtt gtagctgccg ccctggacga tgatgccgat gtcgcttgtg
     3961 ccctcgccaa aaaactcgtt gaggccatgc tgctcgatgt agcgcagcgc cgcaggccag
     4021 cgctcgcgta ctttctcctg ctcgtgcacg aagctcgccg gcggcagcac gatgcggctc
     4081 acgtcgcgct gcgggttctc catagcgtct ttcagggtga acgtgggcgc cttgttgtct
     4141 gcggccacga attcgccatg cacgtggcag gcacgaaggc gcagttgcag catcaccggc
     4201 gtattgctgg cttccgacag tgcaaagccg tgcttgaccg cgccgacgat gctgggcagg
     4261 ttgggccgcg ggtcgagcag ccagatctgc gacttcatcg cgaaggcatg gctgcgctcc
     4321 tgcatgatcg aggagccctc gccgtaatcc tcgcccacca cgatcagcgc cccgccggtg
     4381 acgccgcccg aggccaggtt ggccagcgcg tcggacgcca cgttggtacc gactgtcgac
     4441 ttgaatgtca ccgcgccgcg cagcgggtag ttgaccgagg ccgcgagcgt ggcggcagcc
     4501 gtcgcttcgc tggcgctgtt ctcgaagcgg atgccgtatt cgcccaggat gtcctgcgcg
     4561 tcggccagca cgtccatcag gtgggagatc ggagcgccct ggtagccggc cacgtaggcc
     4621 acgcccgatt ccagcagagc cttggtcacc gccagaatgc cctcgccgcg gaacacctca
     4681 ccggtgccca gcctgagctt cttgacttct tcgacgaacg aacgctctgc cattcacact
     4741 ccagcgacaa gggggcggag gttgacggag gtcaaacctg aacgccttca attactttag
     4801 accagaacct atgaaaagta atatatttcc gaccatagga cttgtcaagc ggggccgtgc
     4861 aaaatagccc catgggcaag caggacatgg ccacggccgc gcatttcgtc gacgactacc
     4921 tgccggcgct gctggcgcag gcgacacagc tgatctcggg cgagttccat cgcgttgtaa
     4981 aggccagggg cgtcaccgtc tccgaatggc gggtgctggc cacactggcc ggcggcgaac
     5041 cgctgagcat cggccgcctc gcccagatca cggtgaccaa gcagcccacg ctcacccgcg
     5101 tgctcgaccg tatggaggcg cgcggcgaag tcaggcgcat cgcgcacgcg gaagaccggc
     5161 gtgtcacgct ggtgggcatc acgcccgccg gcagccggct ggtgacgggg ctgatcaagc
     5221 tggcgcgcga gcatgagcgc cgcgtgctgg agcctttcgg cctccagcgg gcagcggatc
     5281 tcaagaccac gctgaggcgg atgatcgagc tgcacaaggg gctcgccaat gacgatgcgg
     5341 gcgacgagga gtgaccctct cgcgcggcgg ccttacggca gtgtgtcctc cgccggcggc
     5401 cgccacagcc ccgcgtgcct gagcatgccc agcgtgcggg ccagcacgtc cagccggtag
     5461 gcggccacgt cgcggccgct gtagtcgtag aaaccgccgc cggtcttcag tcccagccgg
     5521 ttgtccgcca ccatcttgcc gatcaccccg ggcgccgtat aacgcgcggc atcgatcgaa
     5581 cccgacatct cgcggctcgc atggtgcagg atgtcggcgc cgccgaagtc gatgaactcg
     5641 accaccccca gcgcggcgaa gcgcaggccc aggccaaagc gagtggcctt gtcgatctcc
     5701 tcggggctgg ccacgccctc ttccaccatg cgcgccgcct cgttcatgat gagtgcctgc
     5761 aggcgcggca cgatgtaacc gggcgccacg ccgcacacca ccggcagctt gccgatggat
     5821 tccatcacct cgcgggtgcg cgcaactgcc gcggcgttgg tcttcggatg cgtgctcagc
     5881 tccaccaccg ggatcaggta ggccggattg agccagtgca tgttgaagaa ccgctccggg
     5941 cggcgcacca gccccgccag ctgcgtcacc aggatgctgc tggtggtgga ggtgagaacg
     6001 gcgtcgccgc ggcaatggcg attgaggtgt tcgaaggcgt cacgcttggc ttccatcgtt
     6061 tcggggacgc cttcgaacac cagatccgcc gcggccagcg cctgcggtgc cgcggcggcg
     6121 tcgacgaatt caacgcgcgc ggcgatggca ccgatctggt cggcccgcat ggcgcccagc
     6181 tgggccaggc tgtggagact gcccgcgatc tcggcgcgcg catcgtcgca cagcttctgc
     6241 cacgcctcgc gcgtgcgcgg cttgaggtcg acgagtgcaa tgcgatggcc cgcgtaggcg
     6301 aacgcgatgg cgatgccgcg tcccatgcgg ccggcgccga cggcaacaaa attcttcatt
     6361 cgcctattcg ccgtcgtgca gccgctgccg caggtcttgc gggctgagct gggccaggcc
     6421 gagcgcttcg aagctgcgcg ggccacgccg caggtcgcgc ccgaggaagc caccgaccac
     6481 ggccagcaag ccatgcgcca gcggcgcgtc cacaccggcg tagcgcgcgg ccgaggccag
     6541 aaaggccagc cccagctcgc tgtcttccgt gatgtagcgg tgcgtgcgaa gatcgatgtg
     6601 ttcgcgccag tcgcccgact tcaccagctt cttgtgcgcg tcgccgtaca tccagcggtc
     6661 attgtcgtag tgatcggcca gcgggtagtg cggcgcgccg taacccagcg catcgcgcac
     6721 tgcgatgcgc tcggcatcga gccggtcggt caccgcgcgc acggccggct gcgtgccttc
     6781 attgtgaatg tcccagcgct cgaagtgctg cagcggcgcc gcattcatca cgatgagcgg
     6841 cggatggatg atggggccgg cgttcatcag ggcacccgac agtgcgtcgc cgcagccgtg
     6901 cacgctggga taggcctggc gaatcaccgt cagcgcgtgc tgcgcaccgc gctccgggta
     6961 cacgccggtg ggcagccgca cggcgcggat cgtcacgttc acctcgcgct cgccgtgctt
     7021 gcgcgcgaga taaggcagcg tgccggtttc ggcccacgcg acgtcggaac ggctgcccgc
     7081 tccgcgcacg aggcgcgcca tgacatagct gccgaagctg cccggtgcga ggaaaacaac
     7141 ctggccgtcg ctcaggtgcg gggccatctg ccgcgcgata tcggcttgcg ccgtcgccgg
     7201 cgaaggaatc aggatcagct gcgcgccttt gagcgcctgg ccgatatcgg ccgtggccaa
     7261 ggcaatgccg acttcacgcg cgccggcgct gtccttcagc acgatgccgc cgctgtcgac
     7321 gacgctctgc agggccgccg catcgcgccg ccacaaccgg atctggtggc cctgttcgga
     7381 caagtcggcg gcggcggcgt agcagccgtg gccgccgccc aggatggcga tattcatggg
     7441 gaatgcggtt cttcgagaaa ggctactaca ttacctttca tatattctga tgtcaattat
     7501 cgccgcggcc cgagcctcgc ctgctagccc agcttgagca gcttgcgcgc attgccctca
     7561 tagagggcat gccgctgcgc gggcgtgatg tccagcgaat cgatgatctc gatggtccac
     7621 ttcgtgtagg ccgaaccttt ttccggatcg aagggcgcgt cggacgagaa caccacatgg
     7681 tccacgccga agaacttcag ccccatctcg gtggccgcgc gtgcgccgaa caaagccgta
     7741 tcggcgtaga acatcctgaa atagtcgagc ggccgcttct tcagcttttt aagcacgagc
     7801 gtgtaatcct cgtcggaggt ccggctgccg agctggtccc agcccgggcc gacgcggccc
     7861 tcgaagtaag ggatcatgcc tcccatgtgg tgcgtgatca ccttgagacc gggatgcgca
     7921 tcgaacaatc cctcgaacac catgtgcgcc atcgcggcac tggtttcgta cggccagccg
     7981 aaggtccacc agatttcata gtggcttttc ttctcgcctt tgtagtccgg gaaatcggcg
     8041 ccgcgcgccg gatgcagcca gatcgggcgg tccagcttcg ccatcaggtc gaacagcggt
     8101 cgcgtttcgg gcgccgtcaa cggcttcccg agcacattgg tgaagacctg aacgcccacc
     8161 gcgcccaact gcgcgatggc gcgctcggtc tcccgcagca atccttgcgg atcgttcatc
     8221 ggcagcgaag cgatgaaggc gggaaaacgt tccgggtgct tgtcgacgag tgcggccatc
     8281 ccgtcgttcg ccagccgcgc catctcggtg gacagcggcg gcggcccgaa cacctcgatc
     8341 gggggcgagc ccaggcagat cacctgctgg tagtcgccga agctgtccat gatcctgaag
     8401 cgttcgtcga ggtcgacgat gcagggaatc tcgcggaccc gcttgtgcat gtctttgccg
     8461 ttcggtgcga cttcgagcat ccgctcgtaa taggcgcggg gaaagatgtg gttgaagatg
     8521 tcgagcttca cgaagcggtt ctcctgggac tcccgggtcg ggtcttgtcg ggaccttctc
     8581 atcgttgcac aggctcattc atcctacaag ggcttccggt ctttcctcaa gggctatgtg
     8641 agcgattggg gtcgtcgcac acgcctcgcg cgcggcaagc gcaagagcgt gagcgtgagc
     8701 gtggtcaagg cacgctgaag gctcggcccg cgcggctatg cggcccgcgc cacggcgtca
     8761 tgcgccaggg ccatcacatc gcgcgggtgc acatccttca ggccatggtc gctccaggtc
     8821 tgccgccagc ggcgcgcgcc cggcagcccg ttgcgcaagc ccagcatgtg tcgggcgatc
     8881 gcgctccagg gtgtaccgcg ggccgcggct tcgcgcgcca tgtactccgt catccgcgtt
     8941 tccaccgact cacgggtacg ctcgccggtc tcggactcgc caaagaattg cgcatcccat
     9001 tgcgcgatcc accaggggtt gtgatacgcc tcgcggccga tcatgacgcc atccacccgt
     9061 tgcagctgcc cggccacctg ctctgctgcc gtgatgccgc cgttcaccgc gatcgtcagg
     9121 tgcgggaagt cgtgcttgag ccggtgcacc atctcgtagc gcaagggcgg aacctcgcgg
     9181 ttctccttgg gcgacaaacc cttgagccag gcattgcggg catggacgag gaatacctcg
     9241 cagccggctt cgctcacggt tcccacgaag tcgcgcatga actcgtagtt ttcgctcttg
     9301 tcgatgccga tgcggtgctt caccgtgacc ggcacccggg tggcatcgac catggccttc
     9361 acgcaatccg ccaccagctt cggctcggcc atcaggcacg cgccgaagga gccgcgctgg
     9421 acccgctcgc tggggcagcc gcagttgagg ttgatctcgt cgtagcccca ttgctcgccc
     9481 agcctggcgc aatgcgccag ttcggccgcg tcgctgccgc cgagttgcag cgccaccgga
     9541 tgctcctcgg ggttgaagtc caggtggcgg ggcacatcgc catggatcag cgcgccggtg
     9601 gtcatcattt ccgtgtaaag aagcgcatga cggctgagca gccgatggaa ataacggcag
     9661 tggcgatcgg tccaatccat catcggagcg acgctcatgc gccatcggct gactgattca
     9721 tccataaagg cttatgtaac cgatcttgtg ttgggggata gtagcttgtg acccaggcag
     9781 cgtatcagct gtgaaccgcg ccacgtcaac cgtcaccggg caaaccggcc attcgaatag
     9841 gccgggcgcg gcgcggcgcg aggaacgagg atttgtaagc gggactcacg ttcggtttct
     9901 ccttcgtact gcaccaatgg atgagcttac gtaagatggc ccatggctac ctcaaactca
     9961 tccattggcg gcgatgacgg attcgagctg accagcccgg ggcaacgcac cggcgaaggt
    10021 gcgggcagcg tactgcctta tctgcacgat tcgctggcaa cgaagcccgg ggagctattt
    10081 ccggcagaac accccaaacc cgccaagtcc ctggccggcc ggatgcgcaa ggcgctcctg
    10141 gatatcagga agccgaagaa gaatcagcgc gggccgcgct gagaaacgcc gccccgctcg
    10201 ttcatgtcgt gggcggcgtg tacttgggcc gcctgccggc agcatgcaca caagtccact
    10261 gagtcagggc cggggccggg gccggcgctc gccgccgcgt cacgacgcca atggggtttc
    10321 tttcacgccc gcgtttgaca actccggggc cgggcatcat ccttcgcgat caggcgcggc
    10381 aaggcgtcct acagggcatt ggaacggcga cttacagccg agccgatgca tttgcgcacc
    10441 aatgagacgc caggttcatc cgaaggagcc gatatgaagc attttgatct cgaacccagc
    10501 atgctgaccc tgcgtggcgt gttctacccg accggctaca tgttcatcat gttccccacc
    10561 gagaaagatg cgcgggacgc cgagcgcgca ctcgaggagc atggctacac gggcgaatcg
    10621 agttcgttgc tgactcccga ggatatccag cagaagatcg cgcgcaccgt cggcaacgcc
    10681 gatatccccc ttccgtcggc cggcaccgaa gccgacacgg tgcgccattt caccgagctg
    10741 gccggcaagg ggcactacgc cctgatgatc catgcaccca ccgcggcgga aaccgaacgc
    10801 atcatggagt tgctcaagga tttcccgatt tcctacgggc agaagtaccg gcacctggtg
    10861 atcgaggatc tggtggaatg agaggctgct gagccagcgg cggaacaatg gagaggaccc
    10921 gctttgtcgg gccttgttcg tgcatcgccg agctggtgac aagatcgaga aggcccgctt
    10981 tggcgggcct tgtccgttgt ggttttaggc gctttgctgt ttcatcgggc catctaggcc
    11041 cgacgaaaca gtaagtcaat ttgggggtcg atccccgcac gcggggcccc tcgcccaccc
    11101 aaattgactt accccatatg caacccccca ttcacggaga agtccgcgcc ggtggaatag
    11161 ccgccatctt ccgaagccag ccaggcgatg atggacgcga tttcactcgg ctcgcccagt
    11221 cgcttgacgg ggatggtggc cacgatcttc tcgagcacat cggcccggat cgccttcacc
    11281 atgtcggtac cgatatagcc cgggctgacg gtgttgacgg tcacgccctt ggtcgccagc
    11341 tcctgcgcga gcgccatcgt gaagccgtgc atgccggcct tcgccgccga atagttggtt
    11401 tgcccagcct ggcctttctc gccgttcacc gagctgatgt tgacgatgcg gccccagcct
    11461 ttttccacca tgtcgcccac cacctgcttg gtgacgttga acatggagtt caggttggtt
    11521 tcgatcaccg cgtcccagtc ttcgcgcgtc atcttcagga acatgcggtc ccgggtgatg
    11581 cccgcgttat tgaccagcac gtcgatggaa ccgtgttcgg ctttggcctt gctgaaggcg
    11641 tcgacggtcg aggtccactc gcccacattg cccaccgatg cgtagaaggt gaagccctgg
    11701 gcctgctgct cgcccagcca cttgccgaag tcgcgcgtcg ggccgcaacc cgcgatgacc
    11761 ttgaagcctt ccttgtgcag gcgctggcag atcgccgtac cgatgccacc catgccaccg
    11821 gtcacatacg caattttctg actcattctt gtctccttgc aatttgctac aaacttgaga
    11881 gcgcttcaag cccgccagat gcgggctgcg ccattttcac gcgtcagggg cgttccaccg
    11941 cgagggaaac ccccatgccg ccgccgatac acagcgcggc cagccctttc ttggcttcgc
    12001 gccgctgcat ctcgtgcagc agcgtcacca ggatgcggca gccggatgca ccaatcgggt
    12061 gaccgatggc gatggcgcca ccgttcacgt tgactttcgc cgggtcgatc gcgagctcct
    12121 tgttgacggc gcaggcctgc gcggcgaaag cttcgttcag ctcgaacagg ttcacgtcct
    12181 gcacattcca gccggcacgt tgcaacgcct tgcgagaagc aggaaccggg cccatcccca
    12241 tggtggccgg gtccagcccg ctggtgccga aggcgacgat gcgcgccagc ggcttcaggc
    12301 ccagggcagc ggcctttttc gcgctcatga cgaccacggc cgcggcgccg tcgttgatgc
    12361 ccgaggcatt gccggcggtc acggtgccgg ttttgtcgaa ggcggcacgc aaaccggcca
    12421 gcgcttcggc gttggtcttc ctgttgacgt actcatccgt atcgaagacc agggggtcgc
    12481 ctttgcgctg cggaatggag accggcacga tctcgtcctt gaacttgccc gcatcctggg
    12541 cggccgtggc cttttgctga ctggccagcg ccagcgcgtc ctgtgcggcc cggtcgatgc
    12601 cgtactgctt ggccacgttc tcggccgtga cacccatgtg gtactggttg taaacgtccc
    12661 acagcccgtc gacgatcatc gaatcgatca tcttccagtc gcccatgcgc tggccgtcgc
    12721 gcgaacccat cagcaggtgc ggcgcggcgc tcatgttttc ctggccaccc gccaccacga
    12781 tctcgctgtc gccccaggcg atcgcctgag ccgccagcat cacggccttg aggcccgagc
    12841 cgcagaccgc gttgatggtc atgcccggca cttccttggg aatgccggcc ttgatcagcg
    12901 cctggcgcgc cgggttctgg ccggcgccgg ctgcgagcac ctggcccatg atgacctcgc
    12961 ccacctgggc cgggtcgagt ttcgcgcgcg caatcgcttc cttgatgacg atcgcgccga
    13021 gttcggcggc cgggatcctg gcgagcgagc cgccgaactt gccgacgccg gtgcgggcgg
    13081 cggcaacgat gacaatgtct tccatgtgtg ttctccagtt gcactcagct gtctgtcatt
    13141 gcggccccgg atcgagtccg gggttgcagc ttccgcaatc cgcggcggcg cgctgttcat
    13201 ccgctgcagg aatcccggat caagtccggg atgacaacct tttccatcag gcccgggcct
    13261 tgacatagcg gccgggcgcc ggctcgatgg ccttgtgctt gcccctgccg tagctcttgg
    13321 gcgcagcgat cagcttgccc gcgtaaccct tgagccagcg tgaccagtcg gtccaccagc
    13381 tgccggggtg ttcagtggcg cccgccagcc attcctcatg actcttgggc aacttgtcgt
    13441 tggtccaata cgagcgtttg ttcttcgaag gcggattgat cacgccggcg atgtggcccg
    13501 aggcgcccat gacgaagcgc ctcttgcccg gcaggtgttt cgtggatgca taggcgccgg
    13561 caatcggcac gatatggtct tcacgcgagc cgtagatgta gaccggcagg tcgacctggc
    13621 gcaggtccac tttctcgccg cagaccgtgc acttgcccgg cttgaccagg ttgttctcga
    13681 aataggtatt gcgcaggtac caggcataca tgggcccggg caggttggtc gagtcgctgt
    13741 tccagtagag caggtcgaag ggcggcggcg tttcaccctt gaggtaattg cccacgacgt
    13801 agttccacac caggtcgttg ggccgcagga aactgaaggt ggaggccagc tcgttgccct
    13861 tgaggaggcc gccgcggccc aggtgcgtct cgcgaaactt gacgaaggcc tcgtcgatga
    13921 aaagatccag cacgccggtg tcgctgaagt ccaggaaact cgtgagcagg gttgccgaag
    13981 ccagcggttc ttctccgcgc gccgcgagca ccgccagc
//
LOCUS       DASWIY010000013         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1485119, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000013 DASWIY010000000
VERSION     DASWIY010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485119"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VGA59_00600"
     CDS             <1..618
                     /locus_tag="VGA59_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9718203.1"
                     /translation="AALSPALRRLALRGTIRSYRKNSVIINEGEVGESLFVLLQGRVK
                     VYSNDSDGREITYNVVEQGDYFAEMWLDGGPRSASVMTLEPSVCSIVGRTALREHLAE
                     EPDFALDLVTQVIRRARAATETARNMALLDVYGRVIVTLESQHGPARPDAPVTLTQIT
                     HQNIANRVGASREMVSRLLKDLEKGGYIELGVKRITLLKKLPARW"
     gene            636..1691
                     /locus_tag="VGA59_00605"
     CDS             636..1691
                     /locus_tag="VGA59_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX9718204.1"
                     /translation="MDHRYGCVCFALPRKLLIHLAEQSDGPHRDLLNAQIDLSSQLRA
                     QRAIRSKARPAPKPEPQPLRRQVFDAQGRTFLPGKLLRDEDDPPTRDKDADEAYENVG
                     IAMQFYKSVLGRDSVDGRGKRIDASVHYGFRFANAMWTGEQMIVGDGDGQHVSSLSRS
                     LGIIAHEFSHGITQHIVRGGLGVVQVPGRPPELKGEAGALNESFSDVFASMIKQWHAG
                     EEVDRADWVLGEDILATGAGKAIRSLKDPGNRRLTWPQDDQIKNFKKYRPTDEAHKAS
                     GIANHAFYLAATSLGGKSWETLGAVWLKAFDRLRARATFLDAAHATMEVATALHGARS
                     ANHEAVKAAWKKVNVLV"
BASE COUNT          382 a          652 c          665 g          329 t
ORIGIN      
        1 gctgccttga gccccgcttt gcggaggctg gcgctcaggg gcacgatccg cagctaccgc
       61 aagaactccg tcatcatcaa tgaaggagag gtgggcgagt cgctcttcgt cctgctgcaa
      121 ggccgggtca aggtgtattc gaacgactcc gacggccgcg aaatcacgta caacgtggtc
      181 gagcagggcg attatttcgc ggagatgtgg ctggacggcg gcccccgctc agcctccgtc
      241 atgacgctgg agcccagcgt gtgttccatc gtcggccgca ccgcgttgcg cgagcacctg
      301 gccgaggaac ccgacttcgc gctggacctc gtgacccagg tgatccgccg cgcgcgggcg
      361 gcgacggaga ccgcgcgcaa catggcactg ctggacgtct acggccgggt gatcgtaacc
      421 ctggaaagcc agcacggccc cgcccggccc gacgctcccg tgacgctcac ccagatcacg
      481 caccagaaca tcgccaaccg ggtcggcgcc tcgcgcgaga tggtcagccg cctgctcaag
      541 gacctggaaa agggcggcta catcgaactg ggcgtcaagc gcatcacgct attgaagaag
      601 ctgcctgcgc gctggtagcc gagggaagcg caagaatgga ccatcgatac ggctgcgtct
      661 gcttcgcact gcccaggaag ctgctcattc acctggcgga gcagtcggat ggaccgcacc
      721 gcgacctgct caatgcccag atcgaccttt cttcccaact gcgggcgcag cgtgccatcc
      781 ggagcaaagc ccggcccgcg ccgaaaccgg aaccgcagcc gctgcgccgg caggtgttcg
      841 atgcccaagg ccgtactttt ctgccgggca agctgctgcg cgacgaggac gatccgccca
      901 cccgcgacaa ggatgccgac gaagcgtacg aaaacgtcgg catcgccatg cagttctaca
      961 agagcgtgct cggccgcgat tcggtggatg gccgcggcaa gcggatcgac gcttcggtgc
     1021 attacggctt tcgcttcgcc aacgcgatgt ggaccggcga gcagatgatc gtcggcgacg
     1081 gcgacggcca gcatgtcagc agtctgtccc gatcgctggg catcattgcc cacgagtttt
     1141 cgcatggcat cacacagcac atcgtcaggg gcggattggg cgtggtgcag gtcccgggcc
     1201 ggccgcccga gctgaagggt gaagccggcg ccttgaacga atccttttcg gatgtgttcg
     1261 ccagcatgat caagcagtgg catgccggcg aagaggtcga tcgcgcggac tgggtcctgg
     1321 gcgaagacat cctggcaacg ggggccggca aggccatccg gtcgctgaag gaccccggca
     1381 atcgcaggct gacgtggccc caagacgacc agatcaagaa cttcaagaaa tatcggccga
     1441 ctgacgaagc gcacaaggcc tcgggaatcg ccaaccacgc gttctacctg gcggccacca
     1501 gcctgggtgg caagtcatgg gagacgcttg gcgccgtatg gctcaaggcg ttcgaccgct
     1561 tgcgggcgcg cgccactttc ctggacgccg cgcatgccac gatggaggtt gccacggcgc
     1621 tacatggggc gcgatccgcg aatcatgaag ccgtcaaggc ggcatggaag aaggtgaacg
     1681 ttttagtctg atagccgagc agcgtactat tcgggcatgg aatgcccgtc ctgtcacacg
     1741 gcaaatcccg aatccaaccg cttttgtgaa aactgcggcc aagcgttgcc gtacaagtgc
     1801 gcggcctgtg ggttcgactg cgcgcctcag gcgagatttt gtggcggctg cggagcttcc
     1861 ctgctccggg ggcttgaggc gccacgcccc gccgcggtgc cttcggcgtc ggcctccggc
     1921 tggggcgagt tgaagcaggc caccgtcctc tttgccgaca tcgtcagttc gaccgagcag
     1981 atcgcccaac tcgatccgga agaagccatg gaccggctgg cgccggcg
//
LOCUS       DASWIY010000014         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1486290, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000014 DASWIY010000000
VERSION     DASWIY010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486290"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VGA59_00610"
     CDS             <1..190
                     /locus_tag="VGA59_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718205.1"
                     /translation="LPSQEIHAVFPSPKLVPTKVTGLIKWLQGKFGEAWWTGEVASSS
                     ARQVSRAAAAPLTAGTDG"
     gene            258..1493
                     /locus_tag="VGA59_00615"
     CDS             258..1493
                     /locus_tag="VGA59_00615"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX9718206.1"
                     /translation="MARTKAVLGAGARLSDYLSASLLARVYPAELVHAVLDEHQRNSQ
                     RIRSFPAVAGVYYCMALSLYPEAAYEEVFAVVAQGLAWAAGAAEPARVAKSSISQLRH
                     KIGAAPLASLMHRACVPLADERVHPEAFYAGLRLVAMDGSTFEVPDEADNVEHFGYPG
                     SRNGHAGYPHAQCAVLVECATHAILDASMGAYRDSEWQVCQPLLRSLKPGMLCLADRG
                     FKAYEYWRQAKQTGAQLLWRCRNDRQLPVHRELADGSYLSVIYPPGKGRRAGAAEAIV
                     VRVIEYALPGLPDAQPRYRLLTTLLDPAVAPALELAALYHERWQVEAVFDELKTHLLQ
                     RRRVLRSKTAELVRQEFCGWVLAHYAVRWLMHQGASGHRMRHAELSFASHVQMMRRTQ
                     PRSGAFPPRAPAAAPTLVR"
     gene            complement(1764..>1979)
                     /locus_tag="VGA59_00620"
     CDS             complement(1764..>1979)
                     /locus_tag="VGA59_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9718207.1"
                     /translation="MLGELIVHPEHQRRRVAAELLARVEARFPGVPLYIKALGEAKHF
                     FLACGYEVPRTEMTVLFKKPTTATFAS"
BASE COUNT          327 a          630 c          670 g          352 t
ORIGIN      
        1 gctgccttca caggagatcc acgcggtctt tccatcgccc aagctggtgc cgaccaaggt
       61 gaccgggctg atcaagtggc tgcaggggaa gttcggcgag gcatggtgga cgggtgaggt
      121 ggcgtcttct tcggcgagac aagtgtcgag ggctgccgcc gcaccgctga cggccggaac
      181 tgacggttag ggcccaatac tgttcgttta ggtagttttg tgccacattt gcacttgttc
      241 caccaacgga gcaagccatg gcgagaacca aggccgtgct gggtgcgggt gcccggctga
      301 gcgactatct cagcgccagc ctgctggcgc gtgtgtaccc ggccgagctg gtgcatgcgg
      361 tgctcgacga gcatcagcgc aatagccagc gtatccgcag cttcccggcg gtggccgggg
      421 tgtactactg catggcgctg agcctgtacc cggaggcggc ctacgaggag gtgttcgcgg
      481 tggtcgcaca gggcctggcg tgggcggccg gcgcggccga gccggcgcgc gtggccaagt
      541 cctcgatcag ccagttgcgc cacaagatcg gcgcggctcc cctggccagc ctgatgcatc
      601 gcgcttgtgt gccgctggcc gatgagcggg tccaccccga ggcgttctat gccgggctgc
      661 gcctggtggc catggacggc agcacgttcg aggttcccga tgaggccgac aacgttgagc
      721 atttcggcta ccccggcagc cgcaacgggc acgccggcta tccgcacgcg caatgcgccg
      781 tgctggtcga atgcgccacg cacgccatcc tggacgccag catgggcgcc taccgcgaca
      841 gcgagtggca ggtttgccaa ccgctgctgc gcagcctgaa gccgggcatg ctgtgcctgg
      901 ccgaccgcgg cttcaaggcc tacgagtact ggcgccaggc caagcagacg ggggcgcaat
      961 tgctgtggcg ctgcaggaac gaccggcagt tgccggtgca tcgtgaactg gccgatggct
     1021 cgtacctgag cgtgatctat ccgccgggca agggccggcg cgcgggcgcg gccgaggcca
     1081 tcgtggtgcg ggtgatcgag tacgcactgc cgggcctgcc cgatgcacag ccgcgctacc
     1141 ggctgctgac caccttgctc gatcccgcgg tggccccggc gctggagttg gcggcgctgt
     1201 atcacgaacg ctggcaggtc gaggccgtgt tcgacgagtt gaagacgcat ctgctgcagc
     1261 gccgacgcgt gttgcgcagc aagaccgccg agctggtgcg ccaggagttc tgcggctggg
     1321 tgctggcgca ctatgcggtg cgctggctca tgcaccaagg cgccagcggc catcgcatgc
     1381 gacacgccga gctgtcgttt gccagccacg tccagatgat gcgccgcaca cagccccgct
     1441 ccggggcctt tccccccagg gcgcccgcgg ctgcgccgac gctggttcgc tgagctgctc
     1501 caaaccagcg ccaagctgct ggccacgcgc accgtcggca aacgctcccc gcgtatggtc
     1561 aagcggcgca actccgtgta cgccgcgcac aatcgggcac tggccatccg ggtacccatc
     1621 gactgcacgc ctcgcgcgct tgacccaccc ccgttgagtg caaaccagtg gtttccgaag
     1681 atcaatgtca tctaagcgaa cagtattggg ttaggcgtcg cacgttgagt ggccgtgagc
     1741 atttgggttt gcaactcttg agattaagag gcgaatgttg ctgtggtggg cttcttgaac
     1801 agcactgtca tttccgttct aggcacctcg tagccgcaag caagaaagaa gtgcttcgcc
     1861 tcgcccagcg cttttatgta caagggtacg cctggaaacc gcgcttcgac gcgggcaagg
     1921 agttccgcgg caactcttcg tcgttgatgc tccggatgaa caatgagttc accgagcat
//
LOCUS       DASWIY010000015         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2965490, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000015 DASWIY010000000
VERSION     DASWIY010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2965490"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1276)
                     /locus_tag="VGA59_00625"
     CDS             complement(155..1276)
                     /locus_tag="VGA59_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEX9718208.1"
                     /translation="MADSILEHFGELPDPRRQAGRRHYLSDLLTIAICAVICGADDFE
                     SMAEFGQSKWEWFKAYLKLPHGIPSHDTFRRVIAALDPEAFERCFVSWMNALAGGSRA
                     QVIPIDGKTLRRSFNRASNKAAVHMVSAWAAENELVFGQLATDAKSNEITAIPRLLEL
                     LDLRGATVTIDAEGCQKNIARKIVDQGGDYVLALKGNQPTMFEEVQLFLDDAINRGDK
                     GTALEFHQSVEGDHGRIETRRLWWTPEVDWFQDRSQWAGLGSFAVVERERTAGGETTC
                     ERHYFISSHDQCGAEFMAHAIRSHWRVENSLHWCLDVSFGEDQCRVRIGHAPENLSRL
                     RRIALSLLKQETKVCKLGIKNKRLRAGWDNDYLLRVLQI"
     gene            1493..>1918
                     /locus_tag="VGA59_00630"
     CDS             1493..>1918
                     /locus_tag="VGA59_00630"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A-modifier LpxR family protein"
                     /protein_id="HEX9718209.1"
                     /translation="MRSAPRRAACLPALLCLLLAAAAPAWAWEPAALPPIGPLGSGDV
                     LPERCTLEPKRDRATCDLALHAISLTLENDAFPPLTTDRDYTGGMALDARFLHDIELE
                     GAGAGEASGTALRIRSVGYYGFGLGQAIYTPHDLGAGTVV"
BASE COUNT          292 a          671 c          607 g          348 t
ORIGIN      
        1 acggccatct tcgtggatat ctcgggctgc gagggcttcg tggacgctct gcgcacgcgc
       61 atcccacccc atcgcctgct cgacctcaac gcgctccagc ccccgccctg acgcgctccg
      121 cgcggcgcaa tcccggtgcc agggcgaacg cattctagat ttgcagcacg cgaagcaggt
      181 agtcgttgtc ccagcccgcc cgcaggcgct tgttcttgat gccgagcttg cagaccttgg
      241 tttcctgttt gagcaggctc agtgcaattc ggcgcaggcg ggacaggttt tccggggcgt
      301 ggccgatgcg cacgcggcat tggtcctcgc caaagctgac gtcaaggcac cagtgcaggc
      361 tgttttccac ccgccaatgg ctgcggatgg cgtgggccat gaattcggca ccgcattggt
      421 cgtggctgct gatgaagtag tggcgttcgc acgttgtttc cccgcctgcc gtgcgttcgc
      481 gctctaccac cgcgaagctg cccaggccgg cccactggga acggtcttgg aaccagtcca
      541 cttccggcgt ccaccagagg cggcgggttt cgatgcggcc gtgatcgccc tcgacgctct
      601 ggtgaaactc cagggccgtg cccttgtcgc cccggttgat cgcatcgtcc aggaatagct
      661 gcacctcctc gaacatcgtg ggctggttgc ctttcagggc cagcacgtaa tccccgcctt
      721 ggtcaacgat cttacgtgcg atgttcttct ggcagccctc ggcgtcgatg gtcacggtcg
      781 ctccgcgcag gtccagcaac tccagcagcc tggggatggc ggtgatctcg ttgctcttgg
      841 cgtccgtcgc gagttggccg aagaccaact cgttctcggc cgcccaggcg ctgaccatat
      901 gaactgcggc cttgttgctg gcccgattga acgagcggcg cagcgtcttg ccgtcaatcg
      961 ggatcacctg cgcgcgcgag ccgccggcca gtgcattcat ccacgagaca aaacagcgct
     1021 cgaacgcctc gggatccagc gcggcaatga cgcgccggaa cgtgtcgtgc gaggggatgc
     1081 cgtgcggcag cttgagatac gctttgaacc attcccactt cgactgtcca aattccgcca
     1141 tcgactcgaa gtcgtcggcg ccgcagatca ccgcgcaaat ggcaatggtg agcaagtcgc
     1201 tcaagtaatg tcgccgcccg gcctgccgtc gtggatcggg gagctcgccg aagtgctcca
     1261 ggatcgaatc tgccatccct ggtctccctg gctcctgggc gcttcatcgc gccaggacca
     1321 acggaccaga ttccgtcgcc gcggtccagc ctaaggccgc atcccgaatg gcaggctaga
     1381 atgcgtttgc cctgccctca ggcggcagca cccgcgcttt gctcagccgg gccgatcggg
     1441 gtagggccag agaacgattg ccgcgtctct ccgcgcccca gacggcccca ccatgcgctc
     1501 tgcaccgcgt cgcgcagcat gcctgcctgc cctgctgtgc ctgctgctgg ccgcagcggc
     1561 gccggcgtgg gcctgggaac cggccgcgct gccgcccatc ggcccgctgg gtagcggcga
     1621 cgtcctgccg gagcgctgca ccctggagcc gaaacgcgac cgcgccacct gcgacctggc
     1681 cctgcacgca atttcgctca cgctcgagaa cgatgcattc ccgccgctca ccacggaccg
     1741 cgactacacg ggcggcatgg cgctggacgc gcgcttcctg cacgacatcg agctggaggg
     1801 tgccggcgcc ggcgaggcgt ccggcacggc gttgcgcatc cgctcggtgg gctactacgg
     1861 gttcggactc gggcaggcca tctacacccc gcacgacctg ggggccggca ccgtggtg
//
LOCUS       DASWIY010000016         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_747612, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000016 DASWIY010000000
VERSION     DASWIY010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747612"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..614
                     /locus_tag="VGA59_00635"
     CDS             159..614
                     /locus_tag="VGA59_00635"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX9718210.1"
                     /translation="MKAKMQNNKDFLAGLLMIGIGGIAFYMALDYPFGSALRMGPGYF
                     PRVLAGIFIAFGIYVGLRGLRTNEKVEGVWGWKALALITVAFAAFGWLMDRVGLIPSL
                     VLLFFVSALAGHEFRLKEVIILATVMTLFAYGVFIYGLGLPYRLFWWDY"
     gene            617..>1812
                     /locus_tag="VGA59_00640"
     CDS             617..>1812
                     /locus_tag="VGA59_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX9718211.1"
                     /translation="MEIFNNLIFGFSVALSLQNLVYCFVGVFVGTAIGVLPGIGPLVT
                     IAMLLPITFNLPPIPALIMLSGIYYGSQYGGSTTSILMNLPGETASVVTCIDGYQMAR
                     QGRAGPALAIAAVSSFFAGCVGTLLIAMFGPPIAEMALKFGAPEYFALMLMALVAAAC
                     LSSGDMVKALAMVVLGLLLGIIGTDVNSGMARYSFGLPELTDGMGFTVVAVGLFAVSE
                     IVKNLESKEHGEVFTSKIKNIMPTFADLKASAMPILRGTAVGAFFGTLPGTGPAISSF
                     SSYMVEKKVAKDPSRFGKGAIEGVAGPEAANNSAAQTAFIPTLTLGIPGSGVMALMLG
                     ALTIQGIAPGPQVMTAKPDLFWGLIASMWIGNLMLVVLNLPLIGLWVSLLKVPYRLLF
                     PAIMAFS"
BASE COUNT          276 a          542 c          598 g          396 t
ORIGIN      
        1 aaaagggggg ttgccgcttg ggagacatac tgtgcctgtg tcctccgtgg gccgtgacgg
       61 gagggttcgc tggttccaaa cgaattgagt accaaggagg gggcgacgcc tttgtgcgct
      121 gattcagcgc cgggccgccc gatccgcacg gaggcgttat gaaggccaag atgcagaata
      181 acaaggattt cctggcgggc ctgctgatga tcggtattgg cggcatcgcg ttctacatgg
      241 cgctggatta tcccttcggc agtgcattgc gcatggggcc cgggtacttc ccgcgggtgc
      301 tcgccggcat cttcatcgcg ttcggaatct acgtcggcct gcgcggcctg cgcacgaacg
      361 agaaggtgga gggcgtttgg ggctggaagg cgctggcgct cattacggtg gcgttcgcgg
      421 cgttcggctg gctgatggac cgcgtggggt tgatcccgtc gctagtattg ttattctttg
      481 tgtccgcgct tgccggccat gagttcaggt tgaaggaagt gatcattctg gcaacggtca
      541 tgaccctgtt tgcgtacggg gtcttcatct acgggctcgg cctgccgtat cgccttttct
      601 ggtgggatta ttgaccatgg aaatcttcaa caacctgatc ttcggtttca gcgtcgcgct
      661 gtcgctgcaa aacctggtct attgcttcgt cggcgtattc gtcggcacgg caatcggcgt
      721 gctgcccggc atcggtccgc tggtcacgat cgccatgctg ctgccgatca ctttcaatct
      781 gccgccgata ccggcactca tcatgctgtc cggcatctat tacggctcgc agtacggcgg
      841 ctccaccacg tccatattga tgaacctgcc gggcgagacg gcctccgtgg tgacctgcat
      901 cgacggctac cagatggcgc gccaggggcg cgccgggccg gcgctggcga tcgcggcggt
      961 cagctccttt ttcgccggct gcgtgggcac gctgctgatc gcgatgttcg gcccgccgat
     1021 tgcggagatg gcgctcaagt tcggggcgcc ggaatacttc gcgctgatgc tgatggccct
     1081 ggtcgccgcc gcctgcctct cgagcggtga catggtgaag gcgctcgcca tggtggtttt
     1141 agggctgctg ctcggcatca tcggcaccga cgtcaactcc ggcatggcgc gctattcttt
     1201 cggcctgccg gagctgaccg atggcatggg tttcacggtg gtggcggtgg gcctgttcgc
     1261 cgtttccgaa atcgtgaaga acctcgaatc caaggaacat ggggaggtgt tcaccagcaa
     1321 gatcaagaac atcatgccga cttttgcgga cctcaaggcc tccgccatgc cgatcttgcg
     1381 cggcacggcg gtcggcgcct tcttcggcac gctgcccggc accgggcccg ccatctcttc
     1441 gttctcgtcg tacatggtgg agaagaaggt ggccaaggat ccgtcgcgct tcggcaaggg
     1501 ggcaatcgag ggcgtggcgg gccccgaggc ggccaacaac tctgcggcgc agactgcgtt
     1561 cattccgacg ctcaccctgg gcatcccggg cagcggggtc atggcgctga tgctgggcgc
     1621 gctcaccatc cagggcatcg cgccgggacc gcaggtgatg accgccaagc ccgacctctt
     1681 ctggggcctc atcgcgagca tgtggatcgg aaacttgatg ctggtggtcc tgaacctccc
     1741 gctgatcggc ctgtgggtga gcctgcttaa agtgccgtac cgcctgctct ttcccgccat
     1801 catggcgttc tc
//
LOCUS       DASWIY010000017         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1858675, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000017 DASWIY010000000
VERSION     DASWIY010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858675"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VGA59_00645"
     CDS             complement(<1..820)
                     /locus_tag="VGA59_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX9718212.1"
                     /translation="MSVVAVVGLGYVGLPLAVEFGKMGKTIGYDLSQTKVESYRRFID
                     PTGEVSSEDLRAAAQLAVTTDPVALAQADFIIIAVPTPVDSVHQPDLTALTGASESVG
                     RHMKRGATVVYESTVYPGATEEVCVPILEKASGMRWKRDFHVAYSPERINPGDKEHTL
                     TRIVKVVAGDTPDTLEKVAALYQSVVTAGVFKASSIKIAEAAKVIENTQRDLNIALMN
                     ELAILFDRIGIDTLEVLQAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKADMIGY"
     gene            complement(890..1738)
                     /locus_tag="VGA59_00650"
     CDS             complement(890..1738)
                     /locus_tag="VGA59_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system-associated ATPase"
                     /protein_id="HEX9718213.1"
                     /translation="MYEAFYHLQAKPFQLSPDPRFFFSSSGHRRAMAYLVYGVHQGEG
                     FIVITGEIGAGKTILANTLARKLASGSVVLAQVVSTQLNADDMVRMVAGTLGVPQDSS
                     KAVLLNRVEQFLLLCHRQGKRVLLIVDEAQNLPAASVEELRMLSNYVSATKPLLQTFL
                     LGQPEFRKTLQSPEMEQLRQRVIASCHLGPLDSAETEAYIVHRLQTVGWHGDPSFSRD
                     AFAAIHECTGGIPRKINILCDRLMLLGRLDEKHAFTGREVAQVIEELPQEYAPANLGV
                     SQKGGR"
     gene            complement(1745..>2028)
                     /locus_tag="VGA59_00655"
     CDS             complement(1745..>2028)
                     /locus_tag="VGA59_00655"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9718214.1"
                     /translation="AASNTIRQTGAGLAWNWRVTPWTTWNLGGSYGRNEFLDSDRVDH
                     LTYIRAGITRQFQPQLFGSLYYRRQQNDSNEIGSSYTENAGIATLQMRF"
BASE COUNT          383 a          625 c          600 g          420 t
ORIGIN      
        1 ggtagccgat catgtcggcc ttgtgcgtca ggtaataggg gtcaacgccg atgcaatgtc
       61 cgcccaccag gcccggccgg aacggcagaa aattccattt cgtgcctgcg gcctgcagca
      121 cctccagcgt atcgatgccg atccggtcga acagaatcgc gagctcgttc atcaacgcga
      181 tattgagatc acgctgagtg ttctcgatca ccttcgcggc ctcggcgatc ttgatgctcg
      241 acgccttgaa taccccagcg gtgacgacgc tctggtatag cgccgctacc ttctcgagcg
      301 tatccggagt gtcgcccgcc accaccttca cgattcgcgt gagcgtgtgc tccttgtcgc
      361 cgggattgat gcgttcgggc gaataagcga catggaaatc ccgcttccac cgcattcccg
      421 aggctttctc aaggatcgga acgcatacct cctcggtggc gccgggatag accgtcgatt
      481 catagacgac agtcgcgccg cgcttcatat gcctgccgac cgactcgctg gcgccggtga
      541 gtgcagtgag atcgggctgg tgcacggaat ctaccggcgt cggcaccgca ataatgatga
      601 aatcggcttg cgcgagtgcc accggatcgg tggtcacagc gagctgggca gcggcgcgca
      661 agtcctcgct cgacacttcg ccggtcggat cgatgaacct gcgatagctt tcaaccttgg
      721 tttgcgacag gtcatagccg atggttttgc ccatcttgcc gaactccacg gcgagcggca
      781 agcccacgta tcccaggcca acgacggcga ctacgctcat aatcccccac tccggttatt
      841 gcctaggggt ggcccaggag tatgggggca ccggcgccaa cggatggcat cagcgtcccc
      901 ctttctggct gacccccaaa ttcgcaggcg catattcctg cggcagctcc tcgatcactt
      961 gggccacttc cctgccggta aacgcgtgct tttcgtccaa gcggcccagg agcatcaagc
     1021 ggtcgcacag aatattgatc ttccttggga taccgccggt gcactcgtga atggccgcga
     1081 aggcgtcccg gctgaacgac gggtccccgt gccagcccac ggtctgcagc cggtgcacga
     1141 tataggcctc ggtctccgcg ctgtccaacg gccccaggtg gcaggaggca atgacccgtt
     1201 gcctgagttg ctccatttcg ggactctgca gcgttttgcg aaactccggc tggcccaaga
     1261 ggaaggtctg aaggaggggt ttggtggcgc tcacgtagtt cgacagcatc cggagttctt
     1321 cgacggatgc cgccggaaga ttttgcgcct cgtccacaat caagagcact cgcttccctt
     1381 gcctgtggca taggagcaga aactgctcca ctcgattgag caacaccgcc ttgctgctgt
     1441 cctggggcac gccgagtgtc ccagccacca tgcgcaccat gtcgtcggcg ttcaattgag
     1501 tgctcacgac ttgggccagg accacgctcc ccgatgcgag tttgcgggca agagtgtttg
     1561 cgagaattgt cttgccagcg ccgatttccc ccgtaatgac gatgaaacct tctccctgat
     1621 gcacgccata taccaggtaa gccatggcgc gacgatggcc gctgctggag aagaagaagc
     1681 gcgggtcggg gctcagctga aagggctttg cctggagatg atagaaagct tcgtacattg
     1741 agcgtcagaa tctcatctgg agggtggcta tcccggcgtt ttcagtgtag ctggatccga
     1801 tctcgttaga atcattctgt tgcctgcgat agtacaagga cccgaaaagc tgcggctgga
     1861 actgccgggt tattcccgcc cgaatatacg tgaggtggtc cacccggtcg ctgtcgagga
     1921 attcgttacg gccgtagctc ccacccagat tccaggtggt ccatggcgtc acgcgccaat
     1981 tccacgcaag gccggccccg gtctgccgga tcgtgttgct cgcggcga
//
LOCUS       DASWIY010000018         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1119124, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000018 DASWIY010000000
VERSION     DASWIY010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119124"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VGA59_00660"
     CDS             complement(<1..263)
                     /locus_tag="VGA59_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718215.1"
                     /translation="MPHAFAATLKTFKIASGKTGKFYSLPKLAERFPKVARLPVSMRI
                     VLESVLRNCDGFAVTEDDVRGLAAWQAESPAKQEIPLKPARVVL"
     gene            complement(449..1633)
                     /locus_tag="VGA59_00665"
     CDS             complement(449..1633)
                     /locus_tag="VGA59_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX9718216.1"
                     /translation="MARQFKILVLNQISANGLKRLPAENYVAAKDIADPDAVLVRSAD
                     MNKMEIPATVKAIGRAGAGTNNIPVKAMSARGVPVFNAPGANANAVKELVLAGMLMAA
                     RNLAPAQRFVEKLDPDSPDLDKTVEDGKKNFAGYELAGQTLGVIGLGKIGCLVADAAI
                     KLGMNVLGYDPEITVDAAWSLPAQVKKAGSVTDVLKNASFVSLHVPLVEATRDLVNEK
                     NLSVVKPGTVLLNFSREGVVNTSGVLAALDGGQLGWYVCDFPGGKILKHPKVIALPHL
                     GASTREAEENCAVMVADQLRDYLEHGNVANAVNFPNVNMDRESRYRVAIANANVPNML
                     GQISTAMANAGLNIHNMVNKSRGDMAYTLVDVDSPVGDPVITALRGIEGVLAVHYLPT
                     SG"
     gene            complement(1626..2708)
                     /gene="serC"
                     /locus_tag="VGA59_00670"
     CDS             complement(1626..2708)
                     /gene="serC"
                     /locus_tag="VGA59_00670"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX9718217.1"
                     /translation="MTRVFNFSPGPATLPEPVLRQAAEEMLDWHGSGMSVMEMSHRGK
                     EFISIHAEAESLLRELLGVPANYKVLFLQGGAIGENAIVPMNLIGKTGKADYISTGDW
                     SKKSIKEAKSYGKVNVAASGEASNFTAIPRQSEWKLDPDASYVHICSNETIGGVEYHW
                     TPDTGTVPLVADMSSNILSRPVDIGKYGLIYAGAQKNMGPSGVTVVIVRDDLIGHALP
                     ICPSAFNYKQQADNDSMYNTPPTYAIYIAGLVFKHVKQQGGLQAMAEHNKAKAGVLYD
                     YLDNSKFYRNPVAPDDRSLMNVPFKLKDDALDDAFLKGAQARGMIQLKGHRSVGGMRA
                     SIYNAMPIEGVKALVAYMKEFEVQHG"
BASE COUNT          502 a          957 c          940 g          601 t
ORIGIN      
        1 agaacgacac gggctggttt cagcgggatc tcctgtttgg ccggcgactc cgcctgccac
       61 gcggccaaac cgcgcacgtc gtcctcggtc acggcaaacc cgtcgcagtt gcgcagcacc
      121 gattccagca cgatgcgcat cgaaaccggc aggcgcgcga ccttcgggaa tcgctcggct
      181 aacttcggca gggagtaaaa cttgcccgtc ttgccagagg cgatcttgaa agtcttgagg
      241 gtggcggcaa aagcgtgggg catctcaacc tctgtgttgt tggttccaat gccattttgc
      301 cgccagaacc ctttgtcatg acgtcaccca cctctttgtc attgcggact cgatccgcaa
      361 tccatgaacc ctgcagtcat ggatcccggg tcgcggcccg ggatgacaat caccagcatg
      421 cgggggccgg gatgacaatc agttgcgatt agccggacgt cggcaggtag tgcacggcca
      481 gcacgccttc gattcccctg agcgctgtaa tgaccgggtc gccgacaggg ctgtccacgt
      541 ccaccagcgt ataggccatg tcgccgcgcg acttgttgac catgttgtgg atgttcaggc
      601 ccgcgttggc catcgcggtg gaaatctggc ccagcatgtt gggcacgttg gcattggcga
      661 tggccacgcg gtagcgtgac tcgcgatcca tgttcacgtt gggaaaattc acagcattcg
      721 cgacgttgcc gtgctccagg tagtcgcgca gctggtcggc caccatgacg gcgcagttct
      781 cctccgcctc gcgggtggac gctcccaggt gcggcagggc gatgaccttc ggatgcttca
      841 ggatcttgcc gccggggaaa tcgcagacat accagcccag ctgcccgccg tcgagggcgg
      901 cgagcacccc cgaggtgttc accacgcctt cgcgcgagaa attcagcaac accgttcccg
      961 gcttgaccac gctcaggttc ttctcgttga ccaggtcgcg cgtggcctcc accagcggca
     1021 cgtgcaggct gacgaagctc gcgttcttaa gcacgtcggt cacgctgccg gccttcttca
     1081 cctgggccgg caggctccag gccgcgtcca ccgtgatctc cgggtcatag cccaggacgt
     1141 tcatgcccag cttgatggcc gcgtccgcga ccaggcagcc gatcttgcca aggccgatga
     1201 cgccgagggt ctggccggca agttcgtagc cggcgaaatt cttcttgccg tcctcgacgg
     1261 tcttgtccag gtcgggcgag tccggatcga gcttttcgac gaaacgctgc gccggcgcca
     1321 ggttgcgcgc ggccatcagc atgcccgcca gcaccagctc cttgacggcg ttcgcgttcg
     1381 cacccggtgc gttgaatacc ggcacgccgc gcgcgctcat ggccttcacc ggaatattgt
     1441 tggtgccggc gccggcgcgg ccgatggcct tcaccgtcgc gggaatctcc atcttgttca
     1501 tgtcggcgga gcgcacgagc acggcatccg gatcggcgat gtccttggcc gcaacgtagt
     1561 tctccgccgg caggcgcttc aggccgttcg cggaaatctg gttcaacacc aggatcttga
     1621 attgcctagc catgctgcac ctcgaattcc ttcatgtagg ccaccagcgc cttgacgccc
     1681 tcgatcggca tggcgttgta gatcgacgcg cgcatgccgc ccaccgagcg atggcccttc
     1741 agctggatca tgcccctggc ctgcgcgccc ttgaggaacg cgtcgtccag tgcgtcgtcc
     1801 ttgagcttga acggcacgtt catgagcgag cgatcgtccg gcgccaccgg gttgcggtag
     1861 aacttgctgt tgtccaggta gtcgtagagc accccggcct tggccttgtt gtgttccgcc
     1921 atcgcctgca ggccgccctg ctgcttgacg tgcttgaaca ccaggccggc gatgtagatc
     1981 gcgtaagtcg gcggcgtgtt gtacatcgag tcgttgtccg cctgctgctt gtaattgaag
     2041 gccgacgggc agatcgggag cgcgtgtccg atcaggtcgt cgcgcacgat caccaccgtg
     2101 acgcccgagg ggcccatgtt cttttgcgcg ccggcgtaga tcaagccgta cttgccgatg
     2161 tcgacggggc gcgacaggat gttggacgac atgtcggcga ccaggggcac agtgccagtg
     2221 tcgggcgtcc agtggtattc gacgccgcca atggtttcat tcgagcagat gtgcacgtag
     2281 gacgcgtcgg ggtcgagctt ccattcactt tgcctgggga tggcggtgaa gttgcttgct
     2341 tcgcccgacg cggccacgtt gaccttgccg tagctcttgg cctccttgat cgatttcttc
     2401 gaccagtcgc ccgtgctgat gtagtcggcc ttgccggtct tgccgatcag gttcatgggc
     2461 acgatggcgt tctcgccgat cgcgccgccc tgcaggaaca gcaccttgta gttggcgggc
     2521 acgcccagca gctcgcgcag caggctctcc gcctcggcgt ggatcgagat gaattccttg
     2581 ccgcgatggc tcatctccat caccgacatg ccggacccgt gccagtcgag catttcctcg
     2641 gcggcctggc gcaggacggg ttcgggcagg gttgcgggac cggggctgaa attgaaaacg
     2701 cgtgtcatgg agtttctctc ttccgtgctg aacaaaaaac cggctatggc gcagcggtag
     2761 cgccaaagtt aaacagcata caagaaaggc gatgactccc acgcaatgcc gccgctcgca
     2821 gcgccggtaa agagcccctc cattgcgcta aatcaggtcc agccgacgcg cggcgcccgg
     2881 cgaaggccga accactcgag ttcgggccga ggtcaagccc gggatgacag cctggatcgc
     2941 ggcctgtcat cttgtgcgct tgcgggccac cggtcgatta cgcgcgaggt aataggagtt
//
LOCUS       DASWIY010000019         9229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2232197, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000019 DASWIY010000000
VERSION     DASWIY010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9229
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232197"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2232
                     /locus_tag="VGA59_00675"
     CDS             <1..2232
                     /locus_tag="VGA59_00675"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9718218.1"
                     /translation="SSDVDSRAVYAAWSTVPLSAGQVRVALPAAPIDAAHRKALIAAL
                     SAGGASLLFGLLLAALAARRVARPLHHLATQGPAGATGRVPVREIALLRDALLRARAE
                     DAAVRDSLQVKAHEFETLFNSSPIGMAFTQDPGCRVIWHNAAMDALIGPRGSHKAGTV
                     RVLHEGRELAPGQQPLQRAAALGETVAGMELEIAIDGRPSTFVIANAVPLHDAGGRPR
                     AAISALVDITQRKLVEERLLATDQQLRESQRLMELAQEAGHVGFFHYRFATDLLLWTP
                     GQCKLFGIDALPAGGLRQWYDRMDAADRDRVEREFLTARALHREKETLEYCVARPDGS
                     SVWLSSRIMLYYDGEGHASQMIGVTVDMTDQKQSERARAQLTERALAARREAEAASRA
                     KDELLTMLSHELRNPLGAISAATDVLDAAAAGSPTAVEAREIIGRQTRNLAHMMNDLL
                     DVGRVIAGKTPLAHDHVDLAAVVRRVRQTLAITHDADDHALRLELDEVWVNGDAVRIE
                     QVVTNLLTNAVKYTPRGGLIEVIVRREDDTAVLVVQDSGTGIAPALLPRVFDLFVQGE
                     RPLHRPAGGLGVGLTLVRRLVELHGGTVGVDSSGEGSRFTVRLPAVMAPASQDKDALP
                     LSRRRKVLVVEDNDDVLAALRSKLELDGHSVSTAADGIEGLTRLLKLRPEVSIVDIGL
                     PGLTGYEVARHARAAGYAGRMIALSGYGAESDAQAALVAGFDAYLVKPVDRDQLRASL
                     SAE"
     gene            2320..3660
                     /locus_tag="VGA59_00680"
     CDS             2320..3660
                     /locus_tag="VGA59_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9718219.1"
                     /translation="MGHALIVEDDVDSAQMMAALVATQGFTAATARSLQEARRKMALQ
                     QPDLVLLDLLLPDGSGMSLLDEQDLIGNSEVVLMTGHATVETTIQALRYGAADYLIKP
                     ISAVQLQSILSRVTRPAVLRAEVDTLNEDLRRTGRFGHLVGRSAPMMQVYEQIARVAG
                     TSVTVFVTGESGTGKELLARTVHDLSRRRARPLLAINCGAISPQLMESEIFGHERGSF
                     TGAERQHQGFFERAHGGTLFLDEITEMPPALQVKLLRVLETGTFTRVGSTTPRETDVR
                     VIAASNRDPLQALASGDLREDLFYRLNVFPVQLPPLRERPEDVPLLVMHFLQEIGQRE
                     GSFKRASPAALERLAQYRWPGNVRELRNVLQRAWVMTAGPEVGDQWLPRDVLADPVRA
                     TGALQIAVGTPLAAMERQMILATLEHFGHNKERTAAALGVSLKTLYNRLKEYGV"
     gene            3850..4074
                     /locus_tag="VGA59_00685"
     CDS             3850..4074
                     /locus_tag="VGA59_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEX9718220.1"
                     /translation="MALQDKPAQGNGSVTQRRSVRGFAAMDPQRQREIASLGGRAAHQ
                     SGHAHEFSSEEARAAGRKRHARDAAPTENR"
     gene            4157..4378
                     /locus_tag="VGA59_00690"
     CDS             4157..4378
                     /locus_tag="VGA59_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718221.1"
                     /translation="MQTAAPAHRYLIARKIAKNLGILREQDCFTAECRMIFSNLSRCW
                     TAKADQLAPQEDRPRGGIGMLRPAVLKGR"
     gene            complement(4391..4597)
                     /locus_tag="VGA59_00695"
     CDS             complement(4391..4597)
                     /locus_tag="VGA59_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718222.1"
                     /translation="MTSSKPTDMERGGRVRRTGHGAASVIPHLNVEFALELSHLSDEM
                     PEDAARDDDPARDASLGAASPGGD"
     gene            complement(5077..5256)
                     /locus_tag="VGA59_00700"
     CDS             complement(5077..5256)
                     /locus_tag="VGA59_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718223.1"
                     /translation="MLRTDHRPEELWLIRVALINARYALSVHGPTTLDFASEKAEIDA
                     ALMLIGFDVRATVRH"
     gene            5564..6232
                     /locus_tag="VGA59_00705"
     CDS             5564..6232
                     /locus_tag="VGA59_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9718224.1"
                     /translation="MAGQADKPVRVVVVDDDAHIRRVIAQELMADPRTLLVGQAGSLR
                     EGRRVVREQDFDVILVDLNLGDGRGYDLIAYVKEVRSSAEAVVVSVLEADEDAMRAFE
                     LGASGFLVKNSWFGGFVQSVLQVANGGASITPALARRLLLRLDRRSGLARFSPAGAQK
                     KLTERERAILRMIADGMTSTEIGEQLGISHMTVNTHVKNIYQKLQVRNRAQAVSCANT
                     WGFL"
     gene            6267..7073
                     /locus_tag="VGA59_00710"
     CDS             6267..7073
                     /locus_tag="VGA59_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9718225.1"
                     /translation="MTWISPKELFATSISLLLCRVLLELVPRIAESVRVPAAFTMLAD
                     FGIAMLLILGFGMQLVGDERAVRAERDRIAADLHDGLGFHLTTALSLAGDARKTDSDA
                     WLAVDLAIVELHSVIHFMHSESVPIVQTLGSVRYWLRKVVERQGLELVWQMHQDIPDN
                     ALAGVPAYHFIKIVQEALSNVLQHAGATMLIVQLSCPPPQGVLLLRIADNGRGIGAPG
                     SKAPGRGLDGMIRRARLLGGTLKIEPVPEGGTCIRLSVPLPCRTSDLARN"
     gene            complement(7107..7448)
                     /locus_tag="VGA59_00715"
     CDS             complement(7107..7448)
                     /locus_tag="VGA59_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718226.1"
                     /translation="MNTPAEFEHCIGDLPTGSAMSDAIDAVARLAEQAERMVGKGENE
                     LAMSEALSATFAYMVAYLHHLAIGHREPHEALSKAAAGIPELETAELDFLHTVSRSLR
                     CTRRSSEVTVH"
     gene            complement(7509..7769)
                     /locus_tag="VGA59_00720"
     CDS             complement(7509..7769)
                     /locus_tag="VGA59_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718227.1"
                     /translation="MNSASLSLSEELNVAHEVDTMDENASPTDPLQSDYEAALAEEQR
                     LWRAIHDPGSKATDRVVAYARWRAAVKRLKDLATPPCPGETE"
     gene            8756..>9229
                     /locus_tag="VGA59_00725"
     CDS             8756..>9229
                     /locus_tag="VGA59_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEX9718228.1"
                     /translation="MARAKHNTPIDLSVAHDLTVGLIERLSCRPGRDQDFLRDSKGNG
                     LRVRVTEAGAKAYVFEAKLNRRTIRRTIGDVRALSIDEARKEARRLQSTLDKGDDPRE
                     LDRQKAEADTAARAERRAKAMRETVTGLEAWKEYVAEGKVAGFTRRGKWSERHAQD"
BASE COUNT         1627 a         2984 c         3083 g         1535 t
ORIGIN      
        1 tccagcgacg tcgacagccg cgctgtctac gccgcctgga gtacggtgcc cctgtcggcc
       61 gggcaggtgc gcgtcgcatt gccggcagct cccatcgatg cggcgcaccg caaggccttg
      121 atcgccgcgc tgtccgccgg cggcgcgagc ctgctgttcg gtctgctgct cgccgcgctg
      181 gccgcgcgcc gggtggcgcg gcctttgcat catctcgcca cgcaagggcc ggccggcgcg
      241 accggccgcg tcccggtgcg cgaaatcgcg ctgctgcgcg acgcgctgtt gcgcgcccgg
      301 gccgaagacg ccgcggtgcg cgattcgctg caggtcaagg cccacgaatt cgaaacgctc
      361 ttcaacagca gcccgatcgg catggccttc acgcaggatc cgggctgccg cgtgatctgg
      421 cacaacgcgg cgatggacgc gctgatcggc ccgcgcggct cgcacaaggc cggcacggtg
      481 cgggtgctgc acgagggccg cgaactggcg cccggccagc agccgctgca gcgcgccgcc
      541 gcgctgggtg aaacggtggc cggcatggag ctggagatcg cgatcgacgg ccggccctcg
      601 actttcgtca ttgccaacgc ggtgccgctg cacgacgcgg gcgggcggcc gcgcgcagcg
      661 atcagcgcgc tggtggacat cacgcagcgc aagctggtgg aggagcgcct gctggccacc
      721 gaccagcaac tgcgcgaaag ccagcgcctg atggagctgg cgcaggaagc cggccacgtg
      781 ggcttcttcc actaccggtt cgcgaccgat cttctgttgt ggacgccggg gcagtgcaag
      841 ctgttcggca tcgacgcgct gccggcaggg ggcctgcgcc agtggtacga caggatggat
      901 gcggccgacc gcgaccgggt ggagcgcgaa ttcctcaccg cccgcgcgtt gcaccgggaa
      961 aaggaaaccc tggagtattg cgtggcgcgg cctgacggaa gctccgtctg gctgtccagc
     1021 cgcatcatgc tgtactacga cggcgagggc cacgcctcgc agatgatcgg cgtcaccgtg
     1081 gacatgaccg accagaagca gtcggaacgc gcgcgcgcgc agctgaccga acgcgcgctg
     1141 gccgcgcgtc gggaggctga agcggccagc cgcgccaagg acgaattgct gaccatgctc
     1201 agccacgaac tgcgcaatcc cctcggtgcg atctcggccg ccaccgacgt gctggacgcg
     1261 gccgcggccg gcagcccgac cgccgtcgag gcgcgcgaga tcatcgggcg gcagacacgc
     1321 aacctcgccc acatgatgaa cgacctgctg gacgtgggcc gggtcatcgc cggcaagacg
     1381 ccgctggcgc acgaccacgt cgacctcgcg gcggtcgtgc ggcgggtcag gcagacgctg
     1441 gcgatcacgc atgacgcgga cgatcatgcg ttgcggctgg agctcgacga ggtctgggtt
     1501 aacggcgacg ccgtgcggat cgagcaagtg gtgaccaacc tgttgaccaa cgccgtgaaa
     1561 tacacgccgc gaggcggcct gatcgaggtc atcgtgcggc gcgaggacga caccgccgtg
     1621 ctggtggtcc aggactcggg gaccggcatc gcgccggcgc tgctgcctcg cgtcttcgac
     1681 ctgttcgtgc agggcgagcg cccgctgcac cggccggccg gcggtctggg cgtcggcctg
     1741 accctggtgc gcaggctggt cgaattgcac ggtggcaccg tgggcgtgga tagttcgggc
     1801 gaaggcagcc gcttcacggt gcgcctgccg gcggtcatgg caccggcgtc gcaggacaag
     1861 gatgcgctgc cgctctcacg gcggcgcaag gtgctggtgg tcgaggacaa cgatgacgtg
     1921 ctggcggcgc tgcgttcgaa actggaactg gacggacaca gcgtcagtac cgccgccgac
     1981 ggcatcgagg gcctgacgcg gcttctcaag ctcaggccgg aggtttccat cgtggacatc
     2041 ggcctgcccg ggctgaccgg ctacgaggtg gcccgccatg cgcgggccgc cggttatgcg
     2101 ggacgaatga tcgcgctgtc cggatacggg gctgaaagcg atgcccaggc agccctggtg
     2161 gcgggcttcg atgcgtatct cgtcaagccg gtggaccgcg accagctgcg cgccagcctg
     2221 agcgccgagt gatgttattc aagatgttac agcgccactg tcgggataag cccaataatg
     2281 gaccgggccc cgttcgaggc ctgccaggga aactgaacaa tggggcatgc actgatcgtc
     2341 gaagacgatg tcgattccgc acagatgatg gcggccttgg tcgccacgca aggattcacc
     2401 gccgccaccg cccgttcgct gcaggaggca cggcgaaaaa tggccctgca gcaacccgac
     2461 ctggtgctgc tggatctgct gctgccggac ggcagcggca tgagcctgct cgatgagcag
     2521 gacctgatcg gcaattcgga ggtcgtgctg atgaccggcc acgcgaccgt ggagacgacg
     2581 atccaggccc tgcgctacgg tgccgccgac tacctcatca agcccatcag cgccgtgcag
     2641 ctgcaaagca ttctttcgcg cgtgacgcgt cccgccgtcc tgcgcgccga ggtcgacacc
     2701 ctgaacgaag acctgcgacg caccggccgt ttcggtcacc tggtgggcag gtcggcgccc
     2761 atgatgcagg tgtacgagca gatcgcgcgc gtggcgggca cctcggtcac ggtgttcgtc
     2821 accggcgaga gcggcaccgg caaggagctg ttggcccgca cggtgcacga tctgagccgc
     2881 cgccgcgcac ggccgctcct ggcgatcaac tgcggcgcga tctcgcccca gctgatggag
     2941 agcgagattt tcggccacga gcggggcagc ttcacgggcg cggaacgcca gcaccagggt
     3001 tttttcgagc gtgcccacgg cggtacgctg ttcctggacg agatcacgga aatgccgccg
     3061 gcgctgcagg tcaagctgct gcgcgtgctg gagacgggca ccttcacgcg cgtgggctcg
     3121 accacgccgc gggaaaccga tgtgcgcgtg atcgcggcca gcaaccgcga cccgctgcag
     3181 gccctggcca gtggcgattt gcgcgaggac ctgttctacc gcctcaacgt gttcccggtc
     3241 cagctgccgc cgctgcgcga gcgtcccgag gacgtgccgc tgctggtcat gcatttcctt
     3301 caggaaatcg gccagcgaga aggctcgttc aagcgtgcat cgcccgccgc cctggagcgg
     3361 ctggcgcaat accgctggcc cggcaacgtg cgcgagctgc gcaacgtgct ccagcgcgcc
     3421 tgggtgatga ccgccgggcc cgaagtgggc gaccagtggc tgccgcgcga tgtcctggcc
     3481 gatccggtgc gcgccaccgg cgccctccaa attgcagtgg gaacaccgct ggcggcgatg
     3541 gagcggcaga tgatcctggc caccctggag catttcggcc acaacaagga acgcacggcc
     3601 gccgccctgg gtgtcagcct gaagaccctc tacaaccggc tcaaggagta cggcgtctag
     3661 gagtatgggc gtgcccgggc cgccgccgcg ggggttcatc cttggtgcgc aagcagcccg
     3721 gctttgccgc aatatttacc gccgagcgag cccggattgg cgcaaaaggc ccaacaacga
     3781 tgcctgcacg gccgggcacg aaccttgcca cggtaagagt tctttcaacc cactcccgaa
     3841 aggacagcca tggctttaca ggataaaccc gcccagggca acggcagcgt gacccagcgc
     3901 aggagcgtgc gcggctttgc cgccatggac ccgcagcgcc agcgtgagat cgccagcctg
     3961 ggcggacggg ccgcgcacca gagcggccat gcccacgaat tcagcagcga agaagcgcgc
     4021 gccgcgggcc gcaagcgtca cgctcgcgac gcggccccga ccgaaaaccg ctgacacgtt
     4081 ttcaccggcc gtgcaacgcg gccgacccgg cccggtgtag gaaacagcgg gccgctcctg
     4141 cgtccagacc gcgttcatgc agaccgccgc cccggcgcat cgttacctga tcgcgcgaaa
     4201 gatcgccaag aacctgggca ttctgcggga acaggattgc ttcaccgcgg aatgccgcat
     4261 gatcttctcc aacctgtcgc ggtgctggac ggccaaggcc gatcagctgg ccccgcagga
     4321 ggaccggccg cgcggcggca tcggcatgct gcggccggcc gtgctcaagg ggcgctaggc
     4381 gaggtcgaaa tcagtcgccg ccggggctgg cggcaccgag gctggcgtcg cgcgccgggt
     4441 cgtcgtcccg ggccgcatcc tcgggcatct cgtcactcag gtgtgaaagc tcgagagcga
     4501 attcgacgtt gaggtgcgga atcacgctgg cggcgccatg tccggtgcga cgcacgcggc
     4561 cgccacgctc catatcggtg ggtttggaac tcgtcattta aggccgagca taaccagccc
     4621 gctcctcggc catgagaggc aaaatccctg cctgggcgta gtctttttcc tactgcacgc
     4681 ctctcggcca aaacgttgca aaggtgtgca aacatttcgc cgtggcaatc ggtaatccgg
     4741 gtccggggtc ggtcagcacc ccttccccct tcgcttgtcc tacgtccaag acagcaatcg
     4801 gcctaccagg accatcggca atcagcccaa gaatggggca tggtcaggcg gcgcaaaccc
     4861 cagcaagaaa aggcaaagcc cgcgggcaac gccgatcgcc cgaatagcgc tgatgacagc
     4921 agctcgggcc gaaactcgga acggcgttcc ggtgaaggcg cgcaaagcgc gcttgcgagg
     4981 atgaagcgcg tcgagcgcga tcggcagtgg tccaggccgg aggatgacgg gtccggggac
     5041 taaaggcgag cgatgttcaa cgcgccggcg cggccatcag tgcctgaccg tggccctgac
     5101 atcgaacccg atcagcatca aggcggcgtc gatctcggct ttttcgctcg cgaagtccaa
     5161 tgtggtgggg ccgtgcacgc tcaatgcgta gcgggcgttg atgagggcga cccggatcag
     5221 ccatagctct tccggccggt ggtcggtgcg cagcatgtcc atcgcataac tttcgcacgg
     5281 ctcgtcctgt cgagcttgtc ggctagtgcc gcaatcaggc gtcggctcgc tccggcctgc
     5341 acttcgcgcc ggccaaaagt cacgggacat ttgtcctatg ctcctcgcga gagttgcccc
     5401 ttgcgggtca tcgctgccgg cgcctacgct gggccgggcc caacagggag aagccatgaa
     5461 acctgcaacg gaaatggaac acgcgtctgg cagcgcagca actcgcggtg ctccggaccc
     5521 gggtgccgtc cgcgagatcc cagcccctgc ctggcctagc ttcatggcag gacaggccga
     5581 caagccggtg cgcgtggtgg tcgtcgacga cgatgcgcac atacgccggg tcatcgcgca
     5641 ggaactcatg gcggacccac ggaccctcct ggtcgggcag gccggttccc ttcgggaggg
     5701 caggcgagtc gtgcgagagc aggatttcga cgtcatcctc gtcgatctga acctgggaga
     5761 cgggcgtggc tatgacctga tcgcctatgt caaggaggtc cgcagcagcg cggaagcggt
     5821 tgtggtctcc gtgctggaag ccgacgagga cgccatgcgc gctttcgagc tgggcgcatc
     5881 gggcttcctc gtgaaaaact cgtggttcgg cggctttgtc cagtcggtgc tccaggtggc
     5941 caatggcggc gcgtcgatca cgccggctct cgcgcggcgg ctgctgctgc ggctggaccg
     6001 ccgcagcggg ctggcacggt tttcccccgc cggagcacag aagaaactga ctgagcgcga
     6061 gcgcgcgatc ctgcgcatga tcgccgacgg catgacgagc accgagatcg gagagcaact
     6121 cggcatcagc catatgacgg tcaataccca cgtcaagaac atctaccaga agcttcaagt
     6181 ccgaaacagg gcgcaggcag tcagttgcgc gaatacctgg ggcttcctct gagatcgacc
     6241 ggccttgctc gccaggagtg cgtcgcatga cgtggatctc gccgaaagag ttgttcgcca
     6301 cgagcatcag cctcctgctc tgccgcgtgc tgctggagct ggtgccacgc atcgccgagt
     6361 ccgtacgggt gccggcggcc ttcacgatgc tggctgactt cgggatcgcc atgttgctca
     6421 ttctcggttt cggcatgcag ctggtcgggg acgagcgcgc ggtgcgcgcg gagcgcgacc
     6481 ggatcgcggc cgacctgcac gatggactgg ggttccactt gaccaccgca ctgtcactgg
     6541 cgggagacgc acgcaagacc gattccgatg cctggctggc cgtggacctg gccatagtcg
     6601 agctgcattc ggtcatccac ttcatgcact cggaaagcgt gcccatcgtg cagaccctgg
     6661 gtagcgtccg ttactggctt cggaaggtcg tcgagcggca gggcctggag ctggtgtggc
     6721 agatgcacca ggatattccc gacaatgcgc tggccggggt gccggcgtac cacttcatca
     6781 agatcgtcca ggaagcattg agcaacgtgc tgcagcatgc gggcgcgacc atgctgatcg
     6841 tgcaactgag ttgcccgccg ccccaggggg tcctgctgct gcgaatcgcg gacaacggtc
     6901 gcggcatcgg cgcgccgggg tcgaaagccc cgggccgggg gctggatggc atgatccgcc
     6961 gcgcgcgcct gctcggcggc acgctgaaga tcgagcccgt cccggaaggc ggcacgtgca
     7021 tccgcctgtc ggtcccgctg ccatgcagga cgtccgacct ggcgaggaat tgacaaactg
     7081 gcggcgaatc gccgcgaagc cgccgatcag tgcaccgtca cctccgagct gcgtcgtgtg
     7141 cagcgcaagc tcctggagac tgtgtgaaga aaatccagtt cggcggtttc cagttctgga
     7201 atacccgctg cagctttcga aagcgcctcg tgcggctcac ggtggccgat ggccagatgg
     7261 tggaggtacg cgaccatgta cgcgaatgtg gcgctgagag cttccgacat cgcaagctcg
     7321 ttttcacctt tgccgaccat gcgctcggcc tgctcagcca gccgggctac ggcatcaatc
     7381 gcatcgctca tcgcgctgcc agtgggtaga tcaccgatgc aatgctcgaa ttcagccgga
     7441 gtgttcattg ttgcttcctc tctgttgtct ttcggcgcga gggttctcca acacggcgtt
     7501 ccccttggct actcggtctc tccgggacac ggtggggtgg caaggtcttt caagcgcttg
     7561 accgcggcgc gccagcgtgc atatgccaca acccgatcag tggccttcga tccgggatcg
     7621 tgaatcgccc gccacagccg ctgctcctcg gccaacgcag cctcgtaatc cgattgcagg
     7681 gggtcggtcg gagatgcatt ttcgtccatg gtgtcgacct catgcgcaac gttcaactcc
     7741 tcggacaaag aaaggcttgc ggaattcatg ctacttccag cgcctcttgc gtttcggctt
     7801 ggccatcgtc tccacgacgc aggatccatt tggtctagtt cggcatgccg actgaccagg
     7861 gacgggtcaa acaccggata tgcgactgaa actgagcctt tctggagtgg gccgggcggt
     7921 gaaaagcgtt aacggtcgcg ctgtcccgat ctggccctgt tccggctccc cctgctgcct
     7981 gcgctgcggg tgccgaagcc tattgaagag ttggcgcgca atcatgagaa ctccttgatt
     8041 agttaatgaa gagttagaca tcgatcaacg atctgcaatc tgcggctagc cgcgcaaaca
     8101 gtgcgctacg ccatgaagag cacagtgccg cacccgcagg gccactctgt cttgctgcct
     8161 tggcctcagc aatcgcttgc cctagctacc tatccacggt gaaaatcagt caagccatcc
     8221 gctcgagtgc gaggcactgc gttgcggctg ggtggcagta cacccgcaaa cctggcttgt
     8281 aagcaaacgg aacgcgcgta acgctatgtt tcgctggctt ccaccaaccc gaccgcgagc
     8341 gcatgaattc ctcaaactgc tcgcaaggcg atggcgagtc gctattcaca cggcccctca
     8401 ccgcctttgc ggcatggtgg tcatgggcgg gacgctgccg ctggcgtgga gacttcgcgg
     8461 tgcgggcgcg tgcttcaatt cttgccagag aacttgtagc gtcgaagcgt tactgctggc
     8521 ggagagggtc ggattcgaac cgacggtacg ggagaaccgt acaccggatt tcgagtccgg
     8581 ctcacgtacc atttgctgat gttgagtgat gtagatagaa tccatataaa tcaacacgtt
     8641 ggccgttttc cctctgaaaa gttgccgagt actgcgttga tctggattcg ccaaaatcct
     8701 gaatttttgg ctacatggtg gctacatgac ttgccaccgg attttttatc cgcaaatggc
     8761 acgtgcaaaa cacaacacac cgattgatct cagcgtggcc catgacctca cggtggggtt
     8821 gattgagcgc ttgtcctgcc gccccggcag agatcaggat ttcttgcgcg actccaaggg
     8881 taacggcctg cgggtacggg tgacggaggc cggcgcaaag gcctatgtgt tcgaagccaa
     8941 gctgaaccgc cgcaccatcc gccgcactat tggcgacgtc cgcgccttga gcattgacga
     9001 ggcccgcaaa gaggcccgcc ggctgcagtc cactctcgac aagggggacg atcctcgcga
     9061 actggaccgc cagaaggcag aggccgacac cgccgcgcgc gccgagcgcc gagccaaggc
     9121 gatgcgggag accgtcacgg ggctagaggc gtggaaagag tacgtagccg agggcaaggt
     9181 cgccggattc acccgccgcg gcaagtggag cgagcggcac gcgcaggac
//
LOCUS       DASWIY010000020         3800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1490401, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000020 DASWIY010000000
VERSION     DASWIY010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490401"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..383
                     /locus_tag="VGA59_00730"
     CDS             3..383
                     /locus_tag="VGA59_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9718229.1"
                     /translation="MFFAPVVRTRAVSPAFRSFDRSFERFVNDAFYTNATRGFNVEQD
                     DKAWTVTVDLPGVAREDLSVNIEGAIVRIETKAEAKRQFKAAYELPQDIDAGASGAKL
                     ENGVLTLTLAKQVPVSNARQIEVK"
     gene            540..1358
                     /locus_tag="VGA59_00735"
     CDS             540..1358
                     /locus_tag="VGA59_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9718230.1"
                     /translation="MPTGAINGVQLYWELSGDGGEPAILVHGSWGDHLNWAQVVPALS
                     TSLQVATYDRRGHSRSERLPGQGSIHHDVADLAALIEHLFGGPAHVIGNSFGAAIALN
                     LALQRPDMLRSLVVHEPPLFSLLEGDPAVQPALAAVNERAAAVVALLQAGQFEAGARQ
                     FVETIAFGPGAWDQLPQATRDIFVFNAATWLDEQQEPGWMTLDLKRLSAFSAPALLSF
                     GGQSPPFFPLVVKRIAQALPRARQNVFQQAGHVPHLSHPAEYVRVVQEFIQGTR"
     gene            1960..3303
                     /locus_tag="VGA59_00740"
     CDS             1960..3303
                     /locus_tag="VGA59_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9718231.1"
                     /translation="MAAIVMACQFLSAPALAAPKVEKPDLRFGFIKLTDMVPLAVAYE
                     KGYFEQEGLFVTLEAQANWKVLLDRVISGELDGAHMLSGQPLGATIGFGTKADVITAF
                     TMDLNGNACTVSNDVWAQMKKGLPMKDGKPVHPNKADALKPVIKSFRDKGTPFKMGMV
                     FPVSTHNYELRYWLAAGGIHPGYYAPDKGDVTGQIGADVLISVTPPPQMPSTLESGTI
                     HGYCVGEPWNQQAVFKGIGVPVVTDYDIRNNVSEKVFGVTKGWAEKNPKTHIAVVKAL
                     IRAGKWLDANNGGNRMEAVKIISKPNYVGADMAVLANSMTGTFEYEKGDKRPAKDFNV
                     FFRYNATYPYQSDAVWYLTQMRRWGQIPDAKPDSWYLDIARKVYRADIYQQAAKELIA
                     EGKMTAADFPDFSKETGFRPVAHTKFVDGIAFDGKKPNAYIDGFKIGLKGRDKPE"
     gene            3310..>3800
                     /locus_tag="VGA59_00745"
     CDS             3310..>3800
                     /locus_tag="VGA59_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718232.1"
                     /translation="MLNQTANFLDKTGLTWFVPFVRLAGGEDPRRQLREIILQIGIPV
                     LAFALFLVVWNALASGVQTSLGAIPGPAQVVSEAKSLIAEHHAEREKATAFHERQKAA
                     RAEALKADPNADTSIRPWTGKPTYFDQILTSLKTVFTGFLIATLVAVPVGILCGLSRT
                     VQAA"
BASE COUNT          742 a         1260 c         1169 g          629 t
ORIGIN      
        1 cgatgttttt cgcccccgtc gttcgcaccc gcgccgtttc gccggccttc cgctcgtttg
       61 accgcagttt cgagcgtttc gtcaacgatg ccttctacac caacgccacc cgcggcttca
      121 acgtggaaca ggacgacaag gcctggacgg tgacggtgga cctgccgggc gtcgcccgtg
      181 aagacctgtc ggtgaacatc gaaggcgcca tcgtgcgcat cgagaccaaa gccgaagcca
      241 agcgccaatt caaggccgcc tacgagctgc cgcaggacat cgacgccggg gccagcggcg
      301 ccaagctcga gaacggcgtg ctgacgctga cgctggccaa gcaggtgccg gtcagcaacg
      361 cgcgccagat cgaagtcaag taaggggaaa agaaagcccg ccggaattca cggcgggctt
      421 tttcctgctc gcgccgccgg ggcccctgat tcggccaccg tcgggctttc aacttcgcct
      481 tgaacagccg ctggcgctgt ctagactgag gcaggtccat caacaagtgg aggcctgcca
      541 tgcccaccgg cgcgatcaat ggtgtccaac tctactggga gctcagcggg gacggcggcg
      601 agcccgccat cctcgtgcac ggctcctggg gcgaccatct caactgggcg caggtggtcc
      661 ccgcgctctc aacgtccctg caggtcgcca cttacgaccg ccgcgggcat agccgcagcg
      721 agcggctgcc cggccagggc agcatccacc acgacgtcgc cgacctcgcg gcgctgatcg
      781 aacacctgtt cggcggccct gcacacgtca tcggcaactc gttcggcgcc gcgatcgcct
      841 tgaacctcgc cttgcagcgg ccggacatgc tccgcagcct ggtcgtgcac gaaccgccgc
      901 tgttcagcct gctcgagggc gaccctgcgg tccagcccgc gcttgccgcc gtgaacgagc
      961 gggccgccgc ggtggtcgcg ctgctgcagg ccggccagtt cgaagcgggc gcccgccagt
     1021 tcgtcgaaac catcgccttc ggcccgggcg cctgggacca gctgccccag gccacgcgcg
     1081 atatctttgt cttcaatgcc gcaacctggc tggacgagca gcaggagccc ggatggatga
     1141 cgctcgatct gaagcggctg tcggctttct ccgctccggc gctgctgagt ttcgggggcc
     1201 aaagcccgcc gttcttcccg ctggtggtga aacgcatcgc gcaagcgctg ccgcgcgcgc
     1261 gccagaacgt gttccagcag gccggccatg ttccgcactt gagtcacccg gctgagtacg
     1321 tccgcgtggt gcaggagttc atccagggta cgcgatgagg gggtcactct ccgacgcttc
     1381 gccgcggcaa tcaaaactgc ccggtcctgg atggccggat ctatcccgga gagcgacgct
     1441 caagtccggg atcacgactc tgccagcgcc cagcgcggct taccaatgca tcgcaccgat
     1501 ttagtgcgga tcgcggcgac aggcgccgtt gcggtgcgtg gccttggcag gtaactgccg
     1561 ggacacacgc acttttgcag gaacggacag ccattagggc cgccacaaga ataataccgc
     1621 tggcacggcc attgcttagt gcctccatca cctgccaggt gcggcccttc attcaacgcg
     1681 gaatgatcgg cgcacccggt agcggtcggc gctttgattc ccggcgcctg cgaccatgat
     1741 tcgagtgtaa acacaacttt tgcagcccaa cggcgggctg cgatggacat cgacgtccgc
     1801 acgaaaacga gcgcaccttg ctcggcttgc ttcgtgcgga cgtttttatt tggtgcgcgc
     1861 tggcgcagaa aagcattttc ataaaggagc atatcgatga aacgaaaatc cacccgacac
     1921 gccctgccgg caagagcagc aaaattagcg cgacccgcgt tggccgcaat cgtgatggcc
     1981 tgccagttcc tgagcgcccc ggcgctcgcg gcgcccaagg tggaaaagcc cgatctgagg
     2041 ttcggcttca tcaagctcac cgacatggtg ccgctggccg tagcgtatga gaaaggctac
     2101 ttcgagcagg aaggcctgtt cgtcacgctg gaagcgcaag ccaattggaa agtgctgctc
     2161 gaccgcgtga tttcgggcga actcgacggc gcgcacatgc tgtccggcca accgctgggc
     2221 gccaccatcg gcttcggcac caaggccgac gtcattacgg ctttcaccat ggacttgaac
     2281 ggcaacgcct gcaccgtttc caacgatgtc tgggcgcaga tgaagaaggg cctgccgatg
     2341 aaggacggca agcctgtgca tcccaacaag gcggatgccc tcaaaccggt catcaagagc
     2401 ttccgcgaca agggcacgcc attcaagatg ggcatggtgt tccccgtttc cacgcacaac
     2461 tacgaattgc gctactggct ggccgccggc ggcattcatc ccggctacta cgcgccggac
     2521 aaaggcgacg tcaccggcca gatcggcgcc gacgtcctga tctcggtcac accgccgccg
     2581 cagatgccct cgacgctgga atcgggcacc attcacggct attgcgtggg cgagccgtgg
     2641 aaccagcagg ccgtgttcaa aggcatcggc gtaccggtgg tcaccgacta cgacatccgc
     2701 aacaacgtat cggaaaaggt cttcggcgtc accaaggggt gggcggagaa aaatccgaag
     2761 acgcacatcg cggtcgtcaa ggcgctgatc cgcgccggca agtggctcga tgcgaacaac
     2821 ggcggcaatc gcatggaggc ggtgaagatc atctccaagc cgaactacgt gggcgccgac
     2881 atggccgttc tggccaacag catgaccggc accttcgaat acgagaaagg cgacaagcgc
     2941 ccggcgaagg acttcaacgt gttcttccgc tacaacgcca cttaccccta ccagtcggat
     3001 gccgtctggt acctgacgca gatgcgccgc tggggccaga tcccggacgc caagcccgac
     3061 agctggtacc tggacatcgc caggaaggtg tatcgcgccg acatctacca gcaggcggcc
     3121 aaggagctga tcgccgaagg caagatgacc gcggccgact ttcccgactt cagcaaggaa
     3181 acgggattcc gtccggtggc acacaccaag ttcgtcgatg gcatcgcgtt cgacggcaag
     3241 aagcccaacg cctatatcga cggcttcaag atcggcctga agggccggga caagccggag
     3301 tagaccgcga tgctcaacca aaccgccaat tttctggaca agacgggctt gacctggttc
     3361 gttccgtttg tccgcctcgc cggcggcgaa gacccgcgcc ggcagctgcg cgaaatcatc
     3421 ctgcagatcg gcatcccggt actggctttc gccctctttc tggtggtgtg gaatgcactg
     3481 gcatcgggcg tgcagaccag cctgggcgcc attcccggcc cggcgcaagt cgtgtcggag
     3541 gccaaaagcc tgatcgccga acaccacgcc gagcgggaaa aggcgacggc cttccacgaa
     3601 cgccagaaag cggcgagggc cgaggcactg aaggccgacc ccaatgccga taccagcata
     3661 cgcccgtgga ccggcaagcc gacctacttc gaccagatcc tcacgagcct gaagaccgtg
     3721 ttcaccggct tcctgatcgc cacgctggta gcggttccgg tgggcatatt gtgcggtttg
     3781 agccgcaccg tgcaggcagc
//
LOCUS       DASWIY010000021         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_385867, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000021 DASWIY010000000
VERSION     DASWIY010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385867"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1594)
                     /locus_tag="VGA59_00750"
     CDS             complement(236..1594)
                     /locus_tag="VGA59_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX9718233.1"
                     /translation="MSRFRPIDRQTEYLLPPSVQDWLPESHLARYVVDVVEGLDLSAL
                     ERAYAGRGSDAYHPAMLLSLLIYGYATGTHSSRKIERATYDSLAFRFIACDQHPDHDT
                     LANFRRRFGEQFADAFVQVLQVARENQFSRFGTVSLDGTKIHANASRHSALSYGHAEK
                     IEAQLKAEVQEMLALAEAADRSSVPEGVDLPEEIKRREDRLAAIAAAKAKIEARAKER
                     FEREQAEYEAKMAKRQAKEAATGKKPGGKPPTPPAPGPRADDQLNLTDEDSRIMKVAD
                     GGFEQCYNAQALVDTESMLVMVPHLTQAGNDKEQVEPMLARIGALPEGLNQPDQLLAD
                     TGFFSERNVQCCQAAGIEPLIAVGRDEHHPDWRSRFEEPAPLERPASHVEQMKHALKT
                     KVGRAAYALRKQTVEPVFGIIKSVMGFRQFLLRGVDNVRNEWTLVCLAWNLKRMAVLR
                     PQ"
BASE COUNT          265 a          591 c          581 g          354 t
ORIGIN      
        1 ttcgtggaac ttcccaagac tgctaccggg aagattcaga agtttcgctt gcgggagcag
       61 gccggcagcc gcgaggcaat cactcgactg gctggctaac ctagcagcct gtcgggcttg
      121 agcaccgccg gcgctgtgcc cgggggcttg atttcacaat gtgagaaacg gtcggcattt
      181 caggttccag tcagcggttt tctgcgcgca atcgcaatgc ccctgttctt ttttctcact
      241 gcggacgcaa tacggccatg cgcttcaagt tccacgccag gcacaccagg gtccattcgt
      301 tgcgcacgtt gtccaccccc cgcagcagga actggcggaa gcccatcacc gacttgatga
      361 tgccgaacac cggctccacg gtctgtttcc ggagcgcgta agccgcgcgc cccaccttgg
      421 tcttgagggc gtgcttcatc tgttcgacgt ggctggccgg ccgctcgagc ggtgccggtt
      481 cttcgaagcg gctgcgccaa tccggatggt gttcatcgcg cccgaccgcg atcagcggtt
      541 cgatcccggc ggcctggcag cactggacgt tgcgctcgct gaagaaaccg gtgtcggcca
      601 gcagttggtc gggctggttg agcccttcgg gcagggcacc gatgcgggcc agcatcggct
      661 cgacctgctc cttgtcgttg cccgcctggg tgaggtgggg caccatcacc agcatcgact
      721 ccgtatcgac cagcgcctgc gcgttgtagc actgctcgaa gccgccgtca gccaccttca
      781 tgatgcgcga gtcttcgtcg gtcagattga gctggtcgtc cgcgcgcgga ccaggcgcag
      841 gcggggtcgg cggcttgcca ccgggcttct tgccggtggc cgcttccttg gcctgacgct
      901 tggccatctt ggcttcgtac tcggcctgtt cgcgctcaaa gcgctccttg gcgcgcgctt
      961 cgatcttggc cttggcggcc gcgatggcgg ccagccggtc ttctcgccgc ttgatctcct
     1021 cgggcagatc cacaccctcg ggtacgctgc tccgatcggc cgcttcggcc agcgcgagca
     1081 tctcctgcac ttcggccttg agctgggctt cgatcttctc ggcgtgccca taggagagcg
     1141 cgctgtgtcg gctggcgttg gcgtggatct tggtgccgtc cagactgacc gtgccaaagc
     1201 gcgagaactg gttctcgcgg gcgacttgca gcacctgcac gaaggcatca gcgaactgtt
     1261 cgccgaaacg gcggcggaaa ttcgccagcg tgtcgtggtc cggatgctgg tcgcaggcga
     1321 tgaagcggaa ggccagcgag tcgtacgtgg cccgctcgat cttgcggctc gaatgtgtcc
     1381 ccgtcgcgta gccgtagatc agcagcgaca gcagcatggc cgggtggtag gcatcgctgc
     1441 cgcggcccgc ataggcgcgc tccagtgcag acagatccag cccttccacc acatcgacca
     1501 cgtagcgcgc caagtgcgat tcgggcagcc agtcctgcac cgacggcggc aacaggtact
     1561 ccgtttggcg gtcgatcggg cggaagcggc tcatgctctc gggttccagg ggcgacgcgt
     1621 cattttacag ggggatagtc cgacaggctg ctagggttcc ggttccccca gccgcgcccg
     1681 gatcacatcg tacttctcgc gcgccaccgg gtcgccctgc tgggccgcct gcaggtacca
     1741 gtgcgccgcc aggcgcggat cggctgcgac gccgtcaccg gcttcgtaca t
//
LOCUS       DASWIY010000022         6072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1865841, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000022 DASWIY010000000
VERSION     DASWIY010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865841"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VGA59_00755"
     CDS             complement(<1..415)
                     /locus_tag="VGA59_00755"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718234.1"
                     /translation="MFTIKATLIHGPYATDKWQGVFEIESESLLEDIHALIQSTLNFD
                     DDHLYEFFIARTESSRARLSFDDENGGLYDTSIDSLFPLPDKKNLYYLFDYGDHWLFK
                     ITKATKTAREPSRDEEYPRLIHESGTRPPQYPSDDE"
     gene            complement(769..1965)
                     /locus_tag="VGA59_00760"
     CDS             complement(769..1965)
                     /locus_tag="VGA59_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEX9718235.1"
                     /translation="MATSLAEVLRHFGPQYLRLHPLSTALARVWRAIVGCRTRALGGQ
                     RLRCDGCGVEQWRWHSCRNRHCPQCQSRDRDAWRAARLAELLDVPYCHLVFTLPHELN
                     ALAAVHARWVYDTLMQCTAATLSEFAANPRWLGGIGAFTLVLHTWTQDLRRHLHVHAL
                     MACGALLPGGDGGVGTWVGPKRSQRFLFPVHALSKVLRGKFLHALQQACLSGALPRDP
                     AVTTLARRQRLQALRRHDWVVYAKTPLAGPAAVLDYLARYTHRTAISNERLVAIAGDK
                     VLLRVRADNTGGKRTIAMDGVQFIGRFLQHVLPSGFKRIRHYGLLAPAAKTQRLASAR
                     QLLAMPAANPQAREDAQAFMRRVAAIEISCCPHCKTGHWRVIEQRLRDPAALAAIVPT
                     ACRGPP"
     gene            complement(1984..2853)
                     /locus_tag="VGA59_00765"
     CDS             complement(1984..2853)
                     /locus_tag="VGA59_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9718236.1"
                     /translation="MTSLRQRMLDALVLRGMASRTQESYIDAVSRLARHYKRSPDALS
                     AEEVRQYLLYLLRDRQRSRSTVNQYGCAFRFLYGTVLGLAGEEFYIPLGIAPQRLPEI
                     LSREEIARLFAATERLGPRTFLMLAYATGLRLSELLHLRTADIDSHADRMCIRVVQGK
                     GGKDRYVPLEADALQLLRAWWRSARPRLWLFAGRDESQPLTATSVQKWYQAARAQAGI
                     TKRGGIHTLRHCYATHMLETGADLYSLSQWLGHRHVSTTARYLHLARPDAPDGARRDP
                     LKLLAALPPIPTD"
     gene            3165..4703
                     /gene="istA"
                     /locus_tag="VGA59_00770"
     CDS             3165..4703
                     /gene="istA"
                     /locus_tag="VGA59_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX9718237.1"
                     /translation="MPTNRVTMRQIRETLRLHLQAGLSYSEVGRALKISKSVVGKYVS
                     LARVAGVGWDTAQTLSDEDLEARLYRPALPRGSHQLAPDFGVVHQELKRPGVTLMLLW
                     EEYATGNPLAYKYTSFCIKYREFALCQQRSMRQIHIAGEKLFVDYAGDTVPIVDAVTG
                     EITQAQIFVATLGASNYTYACATLRQTTADWIGAQVQTLEFIGGVPRLVVPDQTRSLI
                     KTPDRYDPEPNRTYEEFARHYGCAVLAARPAHPRDKPKVEGSVLLVQRWILARLRNRR
                     FFSLAELNRAIAELLVDLNNRPFKKLPGCRRSAFESLDAPALRPLPAERYAISRWKAA
                     KVNIDYHVEFEGHYYSVPHRLVGTKIDVRVTGKLLECFASNQRVAGHAVSTVPGGFTT
                     VPDHMPASHRAHLQWTPAKLIAWGQRIGVSTAAVVTWQLEHRPHPEQGYRACLGLLAL
                     VRRYSAERLEAACTRAMAIRAPNLRSVTNILKCGLDRQPSLFPATSSPAIEHENVRGP
                     DYYH"
     gene            4871..5617
                     /gene="istB"
                     /locus_tag="VGA59_00775"
     CDS             4871..5617
                     /gene="istB"
                     /locus_tag="VGA59_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX9718238.1"
                     /translation="MLNEHTLDQLRSLRLDGMVRAIEEQATSTAAAELSFDERLTLLV
                     QRELAWRDDRRVTRLLKAAKLKVSSACIEDINWRASRALDRSLIATLAGGDWLRHARN
                     LLITGATGSGKTWLACALAHQAARSGFSVLYVRAARLFDELQVAHGDGSFARRLMQLA
                     KLDLIVIDDFAISPIGAAERNDLLELLDDRIGTRSTLITSQLPVKAWHTYLNDPTLAD
                     AILDRVVHSSHKIELKGTRSMRDGDAAQAT"
     gene            complement(5852..>6072)
                     /locus_tag="VGA59_00780"
     CDS             complement(5852..>6072)
                     /locus_tag="VGA59_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718239.1"
                     /translation="HHSRMSTPQDQLIQGVECLRPLMEENGFQFSLGEAGKGSGGGFA
                     TGQFTSGDRALSAYFSDRGRPFQSDRGR"
BASE COUNT         1068 a         2049 c         1986 g          969 t
ORIGIN      
        1 cttcatcgtc ggacggatac tgcggtggcc gagtgcccga ctcatgaatt agcctggggt
       61 actcctcatc acggcttggt tcccgagcag tctttgtggc tttggtgatc ttgaaaagcc
      121 agtggtctcc gtagtcgaat aggtagtaga gattcttctt gtctggaaga gggaacaggc
      181 tatcaatgct tgtgtcatac agacccccgt tctcatcatc aaagcttagt ctggcgcggc
      241 tgctctcggt tcgggcgata aagaactcat agagatgatc gtcatcgaag ttcaaggtgc
      301 tctgaatcag tgcgtgaatg tcctcaagta gagactccga ctcgatttca aacactcctt
      361 gccacttgtc ggtcgcgtac ggtccgtgta tcaacgtcgc tttgattgtg aacaccacga
      421 tcgttcccct atgacgccta acgaacagcg tttatctggc gccgggccag tgccgatcga
      481 aggcacggcg gcggtcggcg acagataaac cttgttgaac ttgaccgccg cgatggcggt
      541 ggagtgggca gtgtaagttt cagctgccgg tactggcaac gccggactgc gtttgggcga
      601 tccgcgacgt gtcgacctgt ccgtgaggag agattgcaag tcgcgcgcga tcgatgatgc
      661 gacggggcgg agaggtccgc cgagccgact gtgcaagcat ggcgcaggag cgcctgggcc
      721 tttgcgtgag ccgaggcaag gcgatgttgc aagggatgcg cgtgcagatc acggcgggcc
      781 tcggcaggcc gtggggacga tggctgcaag ggccgctgga tctctcaggc gttgctcgat
      841 cacgcgccag tgtccagtct tgcagtgcgg gcagcagctg atctcgatgg cggccacgcg
      901 gcgcatgaag gcttgggcgt cctcgcgcgc ctgggggttg gcggccggca tcgccagcaa
      961 ctgccgagcc gatgccaggc gctgggtctt ggccgccggg gccagcaacc cgtagtggcg
     1021 gatgcgcttg aagcccgagg gcaacacatg ctgcaggaac cggccgatga actgcacgcc
     1081 gtccatggcg atggtgcgtt tgccgcccgt gttgtcggcg cgcacgcgca gcagcacctt
     1141 gtcgcccgcg atggccacca gtcgctcgtt gcttatggcc gtgcggtggg tgtagcgagc
     1201 caggtagtcc agcaccgccg ccggtccggc cagcggcgtc ttggcgtaca ccacccagtc
     1261 gtggcggcgt agcgcctgca gacgctggcg gcgcgcaagg gtcgtgacag ccggatcgcg
     1321 cggcagcgcg ccgctgaggc aggcttgttg cagcgcgtgc aggaacttgc ctcgcaagac
     1381 cttggacagc gcgtgtaccg ggaacaagaa gcgctgcgaa cgcttggggc caacccaggt
     1441 gccgacaccg ccgtcgccac ccggcagcaa cgccccgcag gccatcagtg catgcacatg
     1501 caggtggcgc cgcaggtcct gcgtccaggt gtgcagcacc agagtgaagg cgccgatgcc
     1561 gccgagccag cgcgggttgg cggcgaactc gctcagcgtt gcggccgtgc actgcatcag
     1621 cgtgtcgtag acccagcgcg catgcacggc ggccagcgca ttgagttcgt gcggcagcgt
     1681 gaagacgaga tggcagtacg gcacgtcgag caactcggcc agccgtgccg cgcgccaagc
     1741 gtcgcggtcc cggctctggc actgcgggca atggcggttg cggcacgagt gccagcgcca
     1801 ctgctccacg ccgcaaccgt cgcagcgcag gcgctggccg cccagcgcgc gggtgcggca
     1861 gccgacgatg gcgcgccaga cccgcgccag cgcagtgctc agcgggtgca agcgcaggta
     1921 ctgcggcccg aaatgccgca gtacctcggc cagcgaggtg gccacggtcg atcagtggcc
     1981 agatcagtca gtggggatcg gcggcagcgc ggccagcagc ttcaacggat cgcgccgggc
     2041 gccatcgggt gcgtcgggcc gggccaggtg caggtagcga gcggtggtgc tgacatggcg
     2101 atggccgagc cactgcgaca ggctgtacag gtccgctccg gtctccagca tgtgggtcgc
     2161 gtagcaatgc cgaagcgtat gaatgcctcc gcgcttggtg atgccggcct gggcgcgcgc
     2221 ggcctgatac cacttctgca cactcgtggc ggtgagcggt tgcgactcgt cacggccggc
     2281 gaatagccac agccgcggac gggcgctgcg ccaccaggcg cgcagcagtt gcagcgcgtc
     2341 ggcctccagg ggcacgtagc ggtccttgcc gcccttgccc tggacgacgc ggatgcacat
     2401 gcgatcggcg tggctgtcga tgtcggccgt gcgtagatga agcagttccg acaggcgcag
     2461 acccgtggcg taggccagca tgaggaaggt gcgaggtccg agccgctcgg tcgcggcaaa
     2521 cagccgcgcg atctcctcgc gcgagaggat ctcgggcagc cgctgcggcg cgatgcccag
     2581 cgggatgtag aactcctcgc cggccaatcc cagtacggtg ccgtacagga atcgaaacgc
     2641 gcagccgtat tgattcacgg tggagcgcga gcgctggcga tcgcgcagca ggtatagcag
     2701 gtactgccgt acctcctcgg cgctcagcgc atccggactg cgcttgtaat gcctcgccag
     2761 tcgggatacg gcatcgatgt aggactcctg ggtccgagat gccatgccgc gcaggaccag
     2821 cgcgtcgagc atgcgttgtc gaagtgacgt catcaccaac tcctcgatcg atacgcgggc
     2881 acatgcccgc aaccgtcaag gtacggcgat caacgcagcc gtcttcttcg agtcccaacc
     2941 accgcgtcag cggtttagtt caacgtgttc tagacatcag ctgcgtattt cagcccatcg
     3001 tggacggcat ttcaggctga tcgtggacgg ggtttcagcg tgatcgtgga cggcgtttca
     3061 gtctgatcgt ggacgccgtt tcagcgggat cgtggacgac gggggtggcg cgcaagtgat
     3121 tttctgaccg gcctcaggct caccgatttt tgcggtccga gccgatgcca accaacagag
     3181 tcacgatgcg acaaatccga gagacattgc gcctgcacct gcaggccggg ctgagctaca
     3241 gcgaggtcgg ccgcgcgctc aagatatcca agagcgtggt gggcaagtac gtctcgctgg
     3301 cccgggtggc cggcgtcggc tgggacacgg cccagaccct cagcgacgaa gaccttgagg
     3361 cccggctgta ccgcccggcg ctaccccgcg gtagccacca actcgccccc gacttcggcg
     3421 tcgtgcacca ggagctcaag cgtcccggcg tcaccctgat gctgctgtgg gaggagtacg
     3481 ccaccggcaa cccgctggcc tacaagtaca ccagcttctg catcaagtac cgcgagttcg
     3541 cgctgtgcca gcagcgctcg atgcgccaga tccacatcgc cggcgagaag ctgttcgtcg
     3601 actacgccgg cgacaccgtg cccatcgtcg acgccgtcac cggcgagatc acccaggccc
     3661 agatcttcgt ggccacgctg ggcgcatcga actacaccta cgcctgcgcc acgctgcgcc
     3721 agaccaccgc cgactggatc ggcgcgcagg tgcagacgct ggagttcatc ggcggcgtgc
     3781 cccgcctggt cgtgcccgac caaacccgct cgctcatcaa gacccccgac cgctacgacc
     3841 ccgagcccaa ccggacgtac gaggaattcg cccgtcacta cggctgcgcg gtgctggccg
     3901 cacgccctgc gcacccgcgc gacaagccca aggtcgaggg ctcggtttta ctggtgcagc
     3961 gctggatcct ggcccgcctg cgcaaccgcc gcttcttcag cctggccgag ttgaacaggg
     4021 caatagccga gctgctcgtc gacctcaaca accgcccctt caagaagctg cccggctgcc
     4081 ggcgcagtgc cttcgagtcg ctcgacgcac cggcgctgcg ccccctgccg gccgagcgct
     4141 acgccatcag ccgctggaag gcggccaagg tcaacatcga ctaccacgtc gagttcgagg
     4201 gccactacta cagcgtgccg caccggctgg tgggcaccaa gatcgacgtg cgcgtcaccg
     4261 gcaagctgct ggagtgcttc gcatccaacc agcgtgtggc cggccacgcc gtgagcaccg
     4321 tgcccggcgg cttcaccacg gtgccagacc acatgccggc gtcgcaccgt gcccacctgc
     4381 agtggacgcc ggccaagctc atcgcctggg gccagcgcat cggcgtgagc accgccgcgg
     4441 tagtgacctg gcagctcgag caccgcccgc atcccgagca aggctaccgc gcctgcctgg
     4501 gtctgctggc cctggtgcgc cgctactcgg ccgagcggct ggaggccgcc tgcacgcggg
     4561 ccatggccat ccgcgcaccg aacctgagga gcgtcaccaa catcctcaag tgcggcctgg
     4621 atcgccagcc ctcgctgttc cccgccacgt ccagcccggc catcgagcac gagaacgtgc
     4681 gcgggccgga ctactaccac tgacgccacc gaccccccag ccgtgccgct gcccaacgtg
     4741 gacggcggca ccgggggcgc tggaccggcg gcgtgcacgg cgcccagcag cacatcgcaa
     4801 cgggcctcgc gcaaccaccg ggctgacccc cgaacaccca caccgcaacc caccaaggaa
     4861 gacccacccc atgttgaacg aacacaccct ggaccaactg cgcagcctgc gcctggacgg
     4921 catggtccgc gccatcgaag aacaagccac cagcaccgcc gctgccgagc tgagcttcga
     4981 cgagcggctg acgctgctgg tgcagcgcga actcgcctgg cgtgatgacc gccgcgtgac
     5041 gcggcttctc aaggccgcca agctcaaggt cagctcggcc tgtatcgagg acatcaactg
     5101 gcgtgccagt cgcgcactgg accgcagcct catcgccacg ctggccggcg gcgactggct
     5161 gcgccatgcg cgcaatttgc tgatcaccgg cgccaccggc agcggcaaga cgtggctggc
     5221 gtgtgccctg gcgcaccaag ccgcacgcag cggcttctcg gtgctgtacg tgcgtgcggc
     5281 gcggctgttc gacgaactgc aggttgcgca cggcgacggc agcttcgcgc ggcggttgat
     5341 gcaactggcc aagcttgacc tgatcgtgat cgacgatttt gcgatctcgc ccatcggcgc
     5401 ggccgagcgc aacgatctgc tcgaactact ggacgaccgc atcggcacac gctcgacctt
     5461 gatcaccagc cagctgccgg tcaaggcctg gcacacctac ctcaacgacc cgacactggc
     5521 cgacgcaatc ctcgaccgcg tcgtgcactc aagccacaag atcgagctca agggcactcg
     5581 gtcgatgcgc gacggcgacg ctgctcaggc gacgtgaacc gggcatcgtg cacgtcctcc
     5641 aggccggcaa gaccggacat gagtacatgg cccggtcggg gcgcctgccg gcggccctgg
     5701 caggggcctg aaatctgaag atcaatccaa ccccaaaacc ggccccggca cgaccctgaa
     5761 ggcagagcgt ttcaggctga tcgtggacgg ttgggctaca ttcaaccctc gtgcttgcgc
     5821 gcctccccgg cgcaccctcg acgacgccgt ttcaccgtcc acgatcagac tgaaacgggc
     5881 gtccacgatc gctgaaatac gcactgagcg cgcgatcgcc gctggtgaac tgccccgtag
     5941 caaaccctcc gccggagcct ttgccggcct cgcccaatga gaactgaaag ccgttctctt
     6001 ccatcagcgg gcgcaggcac tccacgcctt ggatcagttg gtcttgcgga gtagacatgc
     6061 gcgagtggtg gg
//
LOCUS       DASWIY010000023         5438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3342509, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000023 DASWIY010000000
VERSION     DASWIY010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3342509"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /gene="rplU"
                     /locus_tag="VGA59_00785"
     CDS             <1..196
                     /gene="rplU"
                     /locus_tag="VGA59_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX9718240.1"
                     /translation="AVGNGSEIKIGTPLVSGATVTATVVAHGKHDKVHIFKMRRRKHY
                     QKRQGHRQQFTELEIGAIAG"
     gene            212..469
                     /gene="rpmA"
                     /locus_tag="VGA59_00790"
     CDS             212..469
                     /gene="rpmA"
                     /locus_tag="VGA59_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX9718241.1"
                     /translation="MAQKKGGGSTRNGRDSQPKMLGVKAFGGELISAGSIIVRQRGTR
                     FHPGTNVGVGKDHTLFALVDGHVAFATKGALNKQTVSVVPE"
     gene            584..1651
                     /gene="obgE"
                     /locus_tag="VGA59_00795"
     CDS             584..1651
                     /gene="obgE"
                     /locus_tag="VGA59_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX9718242.1"
                     /translation="MKFVDEAFIDVAAGDGGNGCVSFRHEKYKEFGGPNGGDGGRGGH
                     VYAVADPNLNTLVDYRFSRRHEARRGEHGMGSDMFGAAGDDIVLKMPVGTIVSDAETG
                     EVLYELLKPGEMITIAKGGDGGFGNMRFKSAINRAPRQKTPGWPGEKKNLKLELKVLA
                     DVGLLGMPNAGKSTLISAISNARPRIADYPFTTLHPNLGVVRVGPEQSFVVADLPGLI
                     EGASEGAGLGHQFLRHLQRTRLLLHLVDIAPFDGADPVAQAKAIVGELRKYDQALYEK
                     PRWLVLNKLDMVPADEREARVKDFVKRFKHKGPVFEISALTHEGCEALARGAFQHIKA
                     QQVAQQPAEYVDPRFAKDAAE"
     gene            1700..2836
                     /gene="proB"
                     /locus_tag="VGA59_00800"
     CDS             1700..2836
                     /gene="proB"
                     /locus_tag="VGA59_00800"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX9718243.1"
                     /translation="MTARKDSSLLRDARRVVVKVGSGLVTNDGRGLDEGAIGEWCRQL
                     AALARDGREVIMVSSGAIAEGMKRLGWTARPHQIHELQAAAAVGQMGLAQMYESKLRE
                     HGMGSAQVLLTHADLADRERYLNARSTLLTLLQLRVVPVINENDTVVNDEIKFGDNDT
                     LGALVANLVEADALVILTDQKGLYSADPRKDPSATFVHEGRAGDPALEVMAGGAGSSL
                     GKGGMITKILAAKRAAGSGASTVIAWGREPDCLLRLARGEAIGTLLVAPTHKNQARKR
                     WIADHLQLRGAVTVDDGAAAKVRADGKSLLPIGMTSVEGEFSRGDVIAVRDQAGAEIA
                     RGLANYASAEARLLCRKPSGAIEGLLGYVAEPEMIHRTNLVVTR"
     gene            complement(2896..3399)
                     /locus_tag="VGA59_00805"
     CDS             complement(2896..3399)
                     /locus_tag="VGA59_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEX9718244.1"
                     /translation="MHLSRVALSLILAASATHAQLAPVDPDWREAEAPPPPALKLEGL
                     IPIEMPRSALRFGVDPASVSLGSDGVVRYVVVATSDAGAVNALYEGLRCNSGEFKVYA
                     RHTPGSGWSVAKDSPWRSLHDGKTASHSLIIARHGACIGRGANRSAAQIVRDLRAPVD
                     TRFNQPQ"
     gene            complement(3389..4033)
                     /locus_tag="VGA59_00810"
     CDS             complement(3389..4033)
                     /locus_tag="VGA59_00810"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX9718245.1"
                     /translation="MLDRDGFRPNVGIILLNQKNQVFWGKRIRTHSWQFPQGGIDRGE
                     TPEQAMFRELHEEVGLKPEHVRIIARTRDWLRYEVPDRYIRRDARGHYKGQKQIWYLL
                     QLTGQDWNLNLRATNHPEFDAWRWNEYWVPLDVVVEFKRGVYEMALTELARFLPRHDH
                     RNRYLRSGMRARDSQEGAAETPPGPGFELPPGATFEPDPQAGLNPMPQENKHAP"
     gene            4126..>5438
                     /locus_tag="VGA59_00815"
     CDS             4126..>5438
                     /locus_tag="VGA59_00815"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX9718246.1"
                     /translation="MKASQFFISTLKEAPADAEVVSHQLMTRAGMIKKLGAGIYSYLP
                     MGLRVIRKVEAIVREEMNRAGAVELLMPVVQPAELWQESGRFQAYGPELLRLKDRHDR
                     DFIIQPTSEEVITDIARQELRSYRQLPRNLYHIQTKFRDERRPRFGVMRSREFIMKDA
                     YSFDRDAAGAKASYENMSRAYRRIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGE
                     DAIVYCPGSDYAANMEKAEALAPQRSRRAPDQPLVKTSTPGRSTCLQVAELLKVPLAT
                     TVKSLVLATDELDRQGEVVTTQVWLVLLRGDHDMNEIKVGKVPGLDGGFRFATGPEIA
                     DHFGTKPGYLGPIGPRKQVKIVADREVAVMADWICGANEEDFHFTGVNWGRDLPEPDA
                     VADLRNIVEGDPSPDGKGPLAIERGIEIGHIFVLGTKYSAPMNAT"
BASE COUNT         1011 a         1743 c         1838 g          846 t
ORIGIN      
        1 ggccgtcgga aacggcagcg agatcaagat cggcactccc ctggtgtccg gcgcgactgt
       61 gacagccact gtggtggcgc acggcaagca cgacaaggtc cacatcttca agatgcgccg
      121 tcgcaagcat tatcagaagc gccaagggca tcgccagcag ttcaccgaac tggaaatcgg
      181 cgcgatcgcc ggttaagcag gagcagcaga aatggcacag aaaaaaggcg gcggctctac
      241 gcgaaacggg cgggactccc agcccaagat gctcggcgtg aaggcattcg gcggcgaact
      301 gatcagcgcc ggctccatca tcgtgcgcca gcgcggcacc cggttccacc ccggcaccaa
      361 tgtcggcgtg ggcaaggacc acacgctgtt cgcgctggtg gacggccacg tggcgttcgc
      421 gaccaagggc gccctcaaca agcagactgt gagcgtggtg cctgagtgag gtaacctgca
      481 agtccagggc cccgaacaaa cgggacggaa aacaaagctc cactttcggg gctaaaacaa
      541 agccccgaat ttcggggctt tgtccattaa tgggttgaag acaatgaagt tcgtggacga
      601 agcctttatc gacgtcgccg ccggcgacgg ggggaacggc tgcgtctcgt tccgccatga
      661 gaagtacaag gaattcggcg gcccgaacgg cggcgacggc ggccgcggcg gccacgtcta
      721 tgcggtggcc gatcccaatc tcaacacgct ggtggactac cgtttttcgc gccggcacga
      781 ggccaggcgc ggcgagcacg gcatgggctc cgacatgttc ggcgcggccg gcgacgacat
      841 cgtgctgaaa atgccggtgg gcaccatcgt cagcgacgcc gagaccggcg aggtgctgta
      901 cgagctgctc aagcccggcg agatgatcac catcgccaag ggcggcgacg gcggcttcgg
      961 caatatgcgc ttcaagagcg ccatcaaccg ggctccgcgc cagaagacac ccggctggcc
     1021 gggcgaaaag aagaacctca agctcgaact caaggtgttg gccgacgttg gcttgctggg
     1081 catgccgaac gccgggaagt cgaccttgat ttcggcgatc tcgaatgcgc ggccgaggat
     1141 cgcggattac cccttcacca ccttgcaccc caacctgggc gtggtgcggg tgggcccgga
     1201 acagagcttc gtggtggcgg acctgcccgg cctcatcgag ggcgcttccg aaggagccgg
     1261 gctggggcac cagttcctgc ggcacctgca gcgcacccgt ttgctgcttc acttggtgga
     1321 tattgccccc ttcgatggcg cggaccccgt cgcgcaggcc aaggccatcg tgggagaact
     1381 gaggaaatac gaccaggctc tgtacgaaaa gccgcgctgg ctggtcctga acaagctgga
     1441 catggtgccc gcggacgagc gcgaagcccg ggtgaaggac ttcgtcaaac gcttcaagca
     1501 caaaggcccg gtgttcgaga tatcggcact gacgcacgag ggctgcgagg ccctggccag
     1561 gggggcgttc cagcacatca aggcgcagca ggtggcccag cagccggccg aatatgtcga
     1621 cccgcgcttc gcgaaagacg ccgcggagtg atttgctact aaaataatag cgcccgcctc
     1681 gatgcgggtc ccgctgaaaa tgactgcaag gaaagattcc agcttgctgc gtgatgcccg
     1741 ccgtgtcgtg gtcaaggtcg gctccggcct cgtcaccaac gacggccggg gcctggacga
     1801 gggcgccatc ggcgaatggt gccgccagct cgccgcgctg gcccgggatg gcagggaagt
     1861 catcatggtg tccagcggag cgattgcgga aggaatgaag cgcctgggct ggacggcgcg
     1921 gccgcaccag atccacgagc tgcaggccgc cgccgccgtg ggccagatgg gactcgcgca
     1981 gatgtacgaa tccaagctgc gagagcacgg catgggctcg gcccaggtgc tgctcaccca
     2041 cgccgacctg gctgaccgcg agcgctatct caacgcccgc tccaccctgc tgacgctgct
     2101 gcagttgcgg gtcgtccctg tgatcaacga gaacgacacg gtggtcaacg acgagatcaa
     2161 gttcggcgac aacgacacgc tgggcgcgct ggtcgccaac ctggtggagg ccgacgcact
     2221 ggtgatcctg accgaccaga aggggctgta cagcgccgat ccgcgcaagg acccttccgc
     2281 caccttcgtg cacgaggggc gggccggcga ccccgcgctc gaggtcatgg cgggcggcgc
     2341 cggctctagc ctgggcaaag gtggaatgat caccaagatc ctggcggcca agcgggcggc
     2401 gggctcgggg gcctccaccg tgatcgcctg gggccgcgaa ccggattgcc tgttgcggct
     2461 ggcgcgcggc gaggcgatcg gcaccttgct ggtggcgccg acccacaaga accaggcacg
     2521 caagcgctgg atcgccgatc acctgcagct gcgcggtgcc gtgacggtgg acgatggcgc
     2581 ggcggccaag gttcgcgccg acggcaagag cctgctgccg atcggcatga cctcggtcga
     2641 gggcgagttc tcgcgtggcg acgtgatcgc cgtgcgcgac caggccggcg ccgaaatcgc
     2701 gcgcggcctg gcgaactacg ccagcgccga ggcgcgcctg ttgtgccgca agccttcggg
     2761 cgcgatcgaa ggcctgctgg gctacgtggc cgagcccgag atgattcatc gcaccaacct
     2821 ggtcgtgacg cggtagacgc agcggggggg cccgcggcgc gcagacgggc aacgccgccg
     2881 gccgcccggg cgccattact gcggctggtt gaatcgcgtg tcgaccggcg cgcgcaggtc
     2941 gcgcacgatc tgcgcagcgg agcgattagc cccacggccg atgcatgccc catgccgcgc
     3001 gatgatcagg ctgtggctcg cggtcttccc atcgtgcagc gaccgccagg gcgagtcttt
     3061 cgcgaccgac catccgctgc ccggggtgtg gcgtgcgtac accttgaatt ccccggaatt
     3121 gcaccgcagc ccttcatata gggcgttcac cgcgccggcg tcgctggtgg cgaccacgac
     3181 atagcgaacg acgccatcgc tgccaagcga aaccgaagcc gggtccacgc cgaaacgaag
     3241 ggccgaccgc ggcatctcga tcgggatcag gccctcgagc ttgagggcgg gaggcggcgg
     3301 tgcttcggct tcacgccagt ccggatcgac gggcgcgagt tgagcgtgcg tggccgacgc
     3361 ggccagaatc agcgataacg caacacggct aaggtgcatg cttgttttcc tgcggcatcg
     3421 ggttcaggcc ggcctgcggg tcgggctcga aagtggcgcc cggcggcagc tcgaatccgg
     3481 ggccgggcgg tgtttccgct gcgccttcct gactgtctcg tgcgcgcatg ccgctgcgca
     3541 ggtaccggtt gcgatggtcg tggcgcggca ggaagcgcgc cagttccgtg agcgccattt
     3601 cgtagacgcc gcgcttgaat tccaccacca catccagcgg cacccagtac tcgttccagc
     3661 gccaggcgtc gaactcggga tggttggtcg cgcgcaggtt caggttccag tcctggcctg
     3721 tgagctgcag caggtaccag atctgtttct ggcccttgta gtggccgcgc gcgtcgcggc
     3781 gaatgtaccg gtcgggcacc tcatagcgca accagtcccg ggtacgggca atgatgcgca
     3841 catgctcggg cttcagcccg acttcttcgt gcaattcccg gaacatcgcc tgctccgggg
     3901 tttctccccg gtcgatgccg ccttgcggaa actgccagct gtgggtgcgt atgcgcttgc
     3961 cccagaacac ctggtttttc tggttgagca ggatgatgcc gacgttgggc cgaaagccgt
     4021 cccggtcaag cataatcaaa ccccaattct taaactgagt tcattatgca cggcgccatg
     4081 cgtgcaggca aagggctcag cggccagccg accgcaccgt ttccgatgaa agcctcccag
     4141 ttcttcatat ccaccctcaa ggaagcaccc gccgacgccg aagtcgtcag ccaccagctc
     4201 atgacccgcg ccggcatgat caagaagctg ggtgccggca tctacagcta cttgcccatg
     4261 ggcctgcgcg tgatccgcaa ggtggaggcc atcgtgcgcg aggaaatgaa ccgcgccggc
     4321 gcagtggagc tgctgatgcc ggtcgtgcag ccggccgagc tgtggcagga aagcggccgc
     4381 ttccaggcct atggacccga gttgctgcga ctgaaggacc ggcatgaccg ggacttcatc
     4441 attcagccga ccagtgagga ggtgatcacc gacattgccc ggcaggagtt gcgcagttac
     4501 aggcaattgc cccggaatct gtaccacatc cagaccaagt tccgcgacga gcgccggccg
     4561 cgcttcggcg tcatgcgcag ccgcgagttc atcatgaagg atgcctacag cttcgaccgc
     4621 gacgcggcag gcgccaaggc cagctacgag aacatgtcgc gcgcctaccg ccggatcttc
     4681 gatcgcttcg gcctgaccta ccgggcggtg gccgctgaca gcggcgcgat cggcggcgac
     4741 ctgagcgagg aattccaggt gatcgcggcc accggcgaag acgccatcgt ctattgcccg
     4801 ggcagcgact acgcggccaa catggagaag gccgaggcct tggcaccgca gagatcgcgt
     4861 cgcgcgccgg accagccgct ggtgaagacg tcgaccccgg gccgcagcac ttgcctccag
     4921 gtcgccgagt tgctgaaggt gccgctggcg acgacggtga agtcactggt gctggcgacc
     4981 gatgaactcg accgacaggg cgaagtggtc acgacgcagg tctggctggt gctgctgcgc
     5041 ggcgaccatg acatgaacga gatcaaggtc ggcaaggtgc cgggactgga tggcggcttt
     5101 cgcttcgcca ccgggcctga gatcgccgat cacttcggca ccaagccggg ctacctcggt
     5161 ccgatcggcc cgaggaagca ggtcaagatc gtcgcggacc gggaagtggc tgtaatggcc
     5221 gactggattt gcggcgcgaa cgaggaggac ttccacttca ccggcgtcaa ttggggccgc
     5281 gacttgcccg aacccgacgc cgtggcggac ctgcgcaaca tcgtcgaagg cgatccctca
     5341 cccgacggca aaggaccgct cgcgatcgag cgcgggatcg agatcggcca catattcgtg
     5401 ctcggcacca agtacagcgc gccgatgaac gcgacctt
//
LOCUS       DASWIY010000024        10911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2235738, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000024 DASWIY010000000
VERSION     DASWIY010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10911
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2235738"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2887
                     /locus_tag="VGA59_00820"
     CDS             <1..2887
                     /locus_tag="VGA59_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9718247.1"
                     /translation="GTWDDASGERRIHAFAPSRLVEGEGFLAAVSIDRSQVAQASFAT
                     LRNQLLALAVTLVVGLAVAWWVGGRVIVGPAKQILATVRRLEHGRLDARVPLPIGRQR
                     GEFSRIGAAFNLMAESLQMRQLDLETELGRSRSAYLVLDLVLNSMQESLLAVTSTGQF
                     LMFNDAAARLFPLNGPALLPQQWAEGFGFYHDDRTTPYHTDELPLVLSTRGESGRLQQ
                     MFVRNALVPEGRLLQCSWQPIRSESGISGGLVVFTDVTELQRLQSEQAAQFEQLCDTQ
                     RKLIEAQRIGRVGNWELDLQSGRLWWSDEVYELFGMRRETFDGSVNAFMDCVHPDDRA
                     VLKAARDSALGEAKVMNVEYRVVKSDGMAWIHEIAEARRNGGDEPVWFGGVVQDITAR
                     KKDEQALLDSERELHGYTLMLQRAAEAAQAITAHPSPENTMQEVADQACRVIGCHQAM
                     VRLAEDDDGSRMVTSVSLSGKYAPWREHETAPVSAGIDAMVRESRGPLRMTQAQLETD
                     PRWREFAGDTGVRAPASGLLALPLVSRSGLNVGLLVVLDKEQGEFTERDEYVAVELAQ
                     LASIAIENARLFTEIRELNAGLEARIAERTAELTRQEQLFRTLAEQAPEVIWNSDATG
                     RRLTFLNRAWYDLVGGTPQDWIGQSGMAAIHPDDRAEVAANGTRAGQTLSTLSGVRRV
                     RAKDGSYHTMSYKAAPVLDELGQVAFWVGIDTDITELKAIEQALRSSNQELQAFSYSV
                     SHDLRAPLGAIGGFSHALTLKLEGHPDERVRHYLARIQAAVEKMEQLIEALLSLAQVV
                     RAPLNYGPVDLGALARETLEGLQMQHPQRKVTVHVQDGLMAQGDARLLRVLMENLLGN
                     AWKFTSHREDALIEVGRLADSSVFSVRDNGVGFDMAYANKLFGAFQRLHTEAEFPGTG
                     IGLATVRRIVTRHQGRVWGESRVGEGATFYFALSETAPPAWLAGDSA"
     gene            complement(2884..4038)
                     /locus_tag="VGA59_00825"
     CDS             complement(2884..4038)
                     /locus_tag="VGA59_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9718248.1"
                     /translation="MNSLEQLLHYPLGDAMPAQGETMVVAQGQGSGLTPAVKWIRMAL
                     PFALDHINLWLLRDEIDGRPGWTVVDCCITRDESKSQWEQIFATQLEGLPVLRVIVTH
                     MHPDHIGLAHWLCKRWDAPLWISATDFNAAHTASHHTSGFGGEGAANFFAAHGLLDTE
                     AITKIRGRATYYPSMVPDIPPSFRRLQDGDRLRIGGRDWRCISGYGHAPEHIALYCEQ
                     ITLPPTAPAPPAPQGGAPDLGAARRSGGLLISGDMMLPRISTNVSVYYIEPESDPLRQ
                     FLESIDKFARLPPDTLTLPSHGKPFQGLHERIRQLHEHHRERLAEVEQACASAPQSAA
                     DMLPVLFKRKLDLHQTTFAMGESVAHLHALWFAGKVRRQRGDDGVYRFAS"
     gene            4156..4563
                     /locus_tag="VGA59_00830"
     CDS             4156..4563
                     /locus_tag="VGA59_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9718249.1"
                     /translation="MATTTYTIGDLAKEFDLTTRAMRFYEDMGLLQPERSGPGGRNRV
                     YSNRDRTRLKLTLRAKRLGLSLTEAREIIDMYDSPRDTGPQLRKFLTVLAQHRKQLEE
                     QMADLQANLDEVRVHEREARALLARAEKSRQAA"
     gene            4611..5072
                     /locus_tag="VGA59_00835"
     CDS             4611..5072
                     /locus_tag="VGA59_00835"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9718250.1"
                     /translation="MTAPRFEPKDPEYAARVYASFARQAAMATIGASLAAVEPGRVVI
                     ELPWAQALTQQHGFLHAGMVATGLDSACGYAAFSLMPADAAVLTIEYKINLLAPAQGQ
                     RFRMEGVVIKPGRTVSVAEGRAWAIDDGREKLIATMACTLMAVIGRAGLRH"
     gene            5507..6685
                     /locus_tag="VGA59_00840"
     CDS             5507..6685
                     /locus_tag="VGA59_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEX9718251.1"
                     /translation="MSFPGLNFQLGEDIDALRDAVRGFAQAEIAPRAAEIDRSDQFPM
                     DLWRKMGEIGVLGITVPEEYGGANMGYLAHMIAMEEISRASASVALSYGAHSNLCVNQ
                     IKRNGNEAQRRKYLPKLISGEHVGALAMSEAGAGSDVIGMKLKAEDKGGHYLLNGSKM
                     WITNGPDADTLVVYAKTEPELGARGVTAFLIEKGMKGFSIAQKLDKLGMRGSHTGELV
                     FQNVEVPAANVLGNLNGGAKVLMSGLDYERAVLAAGPIGIMQAVMDNVVPYIHDRKQF
                     GQSIGEFQMIQGKVADMYTVLQAGRAFLYTVGKNLDMLGAEHVRQVRKDCASVILWCA
                     EKATWMAGEGIQIFGGNGYINEYPLGRLWRDAKLYEIGAGTSEIRRMLIGRELFAETM
                     "
     gene            6948..7610
                     /gene="can"
                     /locus_tag="VGA59_00845"
     CDS             6948..7610
                     /gene="can"
                     /locus_tag="VGA59_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEX9718252.1"
                     /translation="MTSSSLNDLFVHNREWAARMERERPGFFSGLTQQQQPRYMWIGC
                     SDSRVPANQITGLEPGEVFVHRNVANVVVPTDLNCLSTIQFAVDQLKIRHLMVVGHYG
                     CGGVRAALEGVRIGLADNWLQHVKDVREKHSGLLDWVPQDRRLDALCELNVIEQVANV
                     AHTTVMQDAWQRGQRVTLHGWVYGLSDGLLKNLNISVENNEHLGPAYREAVTAVACAR
                     RP"
     gene            7625..9559
                     /gene="aceK"
                     /locus_tag="VGA59_00850"
     CDS             7625..9559
                     /gene="aceK"
                     /locus_tag="VGA59_00850"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEX9718253.1"
                     /translation="MFPQRLDSPLAYGIAKAMMDGFNRHYRLFRTESARAKHRFETAD
                     WHGQQRSQRERIEFYDLRVKEAVTRLEKEFKAGEQPMEVWQQVKLHYIGLLVDHHQPE
                     LAETFFNSVTTKILHRTHFHNDFIFVRPAVSTEYIENDEPAAMPTYRSYYPTRENLCD
                     TIIRIIDNFRLQHPFDDLPRDARYVLEAISARLQHVKLRANFQIQVLSSLFYRNKGAY
                     VVGKIINGFNEVPFALPILHNRSGRLVIDTCLFGEDDMQMLFSFARAYFMVDMEVPSA
                     YVQFLRSLMPRKPRAEIYNALGLAKQGKTLFYRDFLYHLKHSSDKFRIAPGIKGMVML
                     VFDLPSFPYVFKLIKDYYPPQKETTREQIKAKYLLVKQHDRVGRMADTLEYSEVAFAR
                     ERFEDELIEEIRTHAPSQLQISARRNEDGSDGHEEVIIKHLYIERRMIPLNIYLQEAF
                     DAWHDEGQDSLEAGHAHDMIERAVIEYGNAIKDLVSANIFPGDMLFKNFGVTRNGKVV
                     FYDYDEIEYITDCTFRKIPDARTEEEELSAEVWYRVGPKDVFPETFAPFLLGNPAVRE
                     VFMKHHADLLEASFWQSHKERIQAGYVHDVFPYEREKRFSRRYPPAAGGAPSQSPSAV
                     GKWPSRDTRPGRGEGRVTGT"
     gene            9556..10044
                     /locus_tag="VGA59_00855"
     CDS             9556..10044
                     /locus_tag="VGA59_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718254.1"
                     /translation="MNARDAWEYASFVVTALALPFAIAFFAWEQRKERDNEEEEGYQL
                     LSNAYNDFLKVVLANPDLQLRTNEPLSNPTPEQNERMLVIFDMLMSLFERAYLVAYKP
                     KMSETEARRWNSWDDYMREWCRREDFHNALPLLLRGEDPEFQSYLRRIAQEERGTLIQ
                     LS"
     gene            10057..>10911
                     /locus_tag="VGA59_00860"
     CDS             10057..>10911
                     /locus_tag="VGA59_00860"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX9718255.1"
                     /translation="MSDSIVIVGSARTPMGAFQGDFSALSAHDLGGAAIKAAVERAGI
                     SGDAVGEVLFGNCLMAGQGQAPARQAAFKGGLPKSAGAVTLSKMCGSGMKAAMFAHDM
                     LLAGTHEVMVTGGMESMTNAPYLMLKGRSGYRMGHDRIFDHMMLDGLEDAYEAGRSMG
                     TFGEDCAAKYKFTREAQDAFAIASVKRAKAATESGAFATEITPVTVKSRAGEVVVSID
                     EGPGKVKLDKIPTLKPAFKKDGTITAASSSSINDGAAAMVLMKESTAVRYGSKPIARI
                     VGHAMHSQE"
BASE COUNT         2005 a         3521 c         3641 g         1744 t
ORIGIN      
        1 ggggacttgg gacgatgcct cgggcgagcg ccgcattcac gcatttgcgc caagccggct
       61 ggtggagggc gagggctttc tcgcggccgt gagcatcgac cgcagccagg tggcgcaagc
      121 ttcgtttgcc accctgcgca accagctgct ggcactggcc gtcaccctgg tggttggact
      181 ggccgttgcc tggtgggtcg gcgggcgcgt gatcgtgggg ccggccaagc agatcctggc
      241 gaccgtgcgg cggctggagc acggccgcct ggacgcacgc gtacccttgc cgatcggccg
      301 gcagcgcggc gaattttccc ggatcggtgc cgcgttcaac ctcatggctg aatcactgca
      361 aatgcgccag ctcgatctcg agaccgagct gggccgcagc cgcagcgcct acctggtgct
      421 ggacctggtg ctcaacagca tgcaggagag cctgctggcc gtgaccagca ccggccaatt
      481 cctgatgttc aacgacgccg cggcgcggtt gttcccgctg aacggcccag ccttgctgcc
      541 gcaacagtgg gccgaaggct tcggtttcta ccatgacgac cgcacaacgc cgtaccacac
      601 cgacgagctg ccgctggttc tgtccacgcg cggcgaaagc ggacggctgc agcagatgtt
      661 cgtgcgcaac gcgctggtgc ccgaaggccg gctgctgcaa tgcagctggc agccgatccg
      721 cagcgagagc ggcatcagcg gcgggctggt cgtctttacc gacgtaaccg agttgcagcg
      781 gctgcagtcc gagcaggcgg cgcaattcga gcagctgtgc gacacccagc gcaaactgat
      841 cgaggcgcag cggatcggcc gcgtcggcaa ctgggagctg gacctgcaga gcggccggct
      901 ctggtggtcc gacgaggtgt acgagctgtt cggcatgcgg cgcgaaacat tcgacggcag
      961 cgtcaacgcg ttcatggatt gcgtgcatcc ggacgaccgg gcggtattga aggccgcgcg
     1021 cgattccgcc ctgggcgagg caaaggtgat gaacgtcgaa taccgcgtgg tcaaatcgga
     1081 cggcatggcc tggatccatg aaatcgccga ggcgcgacgc aacgggggcg atgagccggt
     1141 ctggttcggc ggcgtggtac aggacatcac cgcccgcaag aaggacgaac aggccctcct
     1201 ggacagcgag cgcgagctgc acggctacac gctgatgctg cagcgggcgg ccgaggcggc
     1261 ccaggccatc acagcccatc catcgcccga aaacaccatg caggaggttg ccgaccaggc
     1321 gtgccgggtc atcgggtgcc accaggcgat ggttcgcctc gcggaagacg acgacgggag
     1381 ccggatggtc acgtcggtgt cgctttccgg gaaatatgcc ccctggcgcg agcacgagac
     1441 ggcgccggtc agcgcgggca tcgacgcaat ggtgcgtgag tcgcgcggcc cgctgcgcat
     1501 gacccaggcg cagctcgaaa cggatccacg ctggcgcgaa tttgccggcg ataccggcgt
     1561 gcgggcgccc gccagcgggc tcctggcgct gccgctggtc agccgcagcg gtctcaacgt
     1621 cggcctgctg gtggtcctgg acaaggagca gggtgaattc accgagcgcg acgaatacgt
     1681 ggcggtggag ctggcgcagc tggcctcgat cgcgatcgag aacgccaggc tgttcaccga
     1741 gatccgcgaa ctcaacgccg gcctggaagc gcgcatcgcc gagcggacgg ccgaactgac
     1801 gcggcaggag cagctgttcc gcacgttggc ggaacaggca cccgaggtca tctggaatag
     1861 cgatgcgacc gggcgccggc tgactttcct caaccgcgcc tggtacgacc tggtgggcgg
     1921 cacgccgcag gactggatcg gccagagcgg gatggccgcg atccacccgg acgaccgcgc
     1981 cgaggttgcg gccaacggga cgcgagcggg ccagaccctg agtaccttaa gcggtgtgcg
     2041 ccgcgtcagg gccaaggacg gcagctacca cacgatgtcg tacaaggccg cgccggtgct
     2101 cgacgagctg ggccaggtcg cgttctgggt cggcatcgat acggacatca ctgaattgaa
     2161 ggcgatcgag caggcgctgc gcagctccaa ccaggaactc caggcgtttt cctattccgt
     2221 gtcgcatgac ctgcgcgctc ccctgggcgc gatcgggggc ttcagccacg cgctgacgct
     2281 caagctcgaa ggacatccgg atgagcgggt ccggcactac ctggcgcgca tccaggccgc
     2341 ggtcgagaaa atggagcaac tcatcgaggc cctgctgtcg ctggcccaag tggtccgcgc
     2401 gccgctgaat tacggcccgg tggacttggg cgcgctggcg cgcgaaacgc tggaggggct
     2461 gcagatgcag caccctcagc gcaaggtgac ggtgcacgtg caggatggcc tgatggcgca
     2521 gggcgacgcg cgcctgttgc gggtgctgat ggagaacctg ctgggcaacg cctggaaatt
     2581 cacttcccac cgcgaggatg ctctcatcga ggtaggccgg ctggccgaca gcagcgtttt
     2641 ttcggtgcgc gacaacggcg tgggcttcga catggcctat gccaacaagc tgttcggcgc
     2701 tttccagcgg ctgcataccg aggcggaatt cccgggcacc gggatcgggt tggccaccgt
     2761 gcggcgcatc gtcacgcggc accagggccg ggtctggggc gagtcgcggg tgggcgaggg
     2821 cgcaaccttc tatttcgccc tgtcggaaac agctcccccg gcctggctgg ccggcgacag
     2881 cgcttaggac gcgaagcggt agacgccgtc gtcgccgcgc tgcctgcgca ccttgccggc
     2941 gaaccacagc gcgtgcaggt gggcgaccga ttcacccatg gcgaaagtgg tctggtgcaa
     3001 atcgagcttg cgcttgaaca gcacgggcag catgtcggcg gccgattgcg gcgcgctggc
     3061 gcaggcctgt tccacctccg ccaaccgctc gcggtggtgc tcgtgcagct ggcggatgcg
     3121 ctcgtgcaag ccctggaacg gcttgccgtg cgatggcagg gtcagggtgt ccggcggcag
     3181 ccgagcgaac ttgtcgatcg attccaggaa ctggcgcagc gggtcggatt cgggttcgat
     3241 gtagtagacg ctgacgttgg tcgatatccg cggcagcatc atgtcgccgc tgatcagcag
     3301 cccacccgat cgacgggccg cccctagatc gggtgcgccc ccctgggggg caggcggcgc
     3361 aggcgccgtg gggggcaatg ttatttgctc gcaatacagg gcaatgtgtt cgggggcgtg
     3421 gccatagccg ctgatgcagc gccagtcgcg cccgccgatg cgtaaccggt cgccatcctg
     3481 gagccggcgg aagctgggcg gaatgtcggg caccatgctc gggtaatagg tggcgcggcc
     3541 gcgaatcttc gtgatcgctt cggtgtccag gagtccgtga gcggcgaaga aattggccgc
     3601 accttcgccg ccgaagccgc tggtgtggtg cgaggccgta tgggcggcgt tgaagtcggt
     3661 cgcgctgatc cacagcggcg catcccagcg cttgcacagc caatgggcca acccgatgtg
     3721 gtccgggtgc atatgggtga cgatcacgcg aagcaccggc aagccttcca gctgggtcgc
     3781 gaagatctgt tcccactgcg acttggattc atcccgggtg atgcagcagt cgaccaccgt
     3841 ccatccgggg cggccatcga tctcgtcgcg cagcagccac aggttgatgt ggtccagcgc
     3901 gaacggcagc gccatgcgga tccatttcac ggccggcgtg aggccgctgc cctggccttg
     3961 cgccacgacc atggtttcgc cctgggcggg catggcgtcg cccagcgggt aatgcagcag
     4021 ctgttcgagc gaattcatgg atgggctggg gcttggcggt cggtaaggat tgacgttgac
     4081 gtaaacgtca tatgtaatcc gcattgtagg gaccagtgca tgcccccact gtcccctgcc
     4141 gtacacgcaa actttatggc cacaaccact tacaccatcg gcgacctggc gaaagagttc
     4201 gacctcacga cccgggccat gcgcttctat gaggacatgg gtctgctgca gcccgagcgg
     4261 tcgggcccgg gtgggcgcaa ccgcgtctac agcaaccgcg accgcacgcg cctgaagctc
     4321 acgctgcgcg ccaagcggct cgggctttcg ctgaccgagg cgcgcgaaat catcgacatg
     4381 tacgacagcc cgcgcgatac cggaccgcag ctgagaaagt ttctgaccgt gctggcccag
     4441 caccgcaagc agctggaaga gcagatggcc gacctgcagg ccaacctgga cgaggtcagg
     4501 gtgcacgagc gggaggcgcg ggcgttgctg gcgcgtgccg agaaatcgcg gcaggccgcc
     4561 tgaccgggca taattgacgt ttacgtaaac gtcaactgaa ggccatcgcc atgaccgcac
     4621 cgcgcttcga gccgaaagac cccgaatacg ccgcccgcgt ttatgccagt ttcgcccggc
     4681 aggcggcgat ggccaccata ggggccagcc tggccgccgt cgagccgggc cgggtcgtga
     4741 tcgagctgcc gtgggcgcag gcgctgactc agcagcacgg attcctgcac gccggcatgg
     4801 tggcgaccgg actggattcg gcctgcggct acgccgcttt cagcctgatg ccggccgatg
     4861 ccgccgtgct gaccatcgaa tacaagatca acctgctcgc gccggcccag ggccagcgct
     4921 tccgcatgga aggggtggtg atcaagcccg gccgcactgt cagcgtcgcc gaaggcaggg
     4981 cctgggcgat cgacgacggc cgcgagaagc tcatcgccac gatggcctgc acgttgatgg
     5041 ccgtcatcgg ccgtgccggc ctgcgccact gagatgaaac ctgtcccaca tgctgctgtc
     5101 atcccgggct tgacccggga tccatgcggc gtcagcgccc tccaccggca tggattgcgg
     5161 gtcgagcccg caatgacaag ctctgcgttt catgcggcgc gggtcgcgcg gcgcgcggtg
     5221 gagcaactga gatgaaagcc cacccccgaa gcgcctgcgg cgatgccccc tcgggcttcg
     5281 ccccctcccc ctcaaggggg ccgagacctg cggctccaaa ccgacctgcg tcggtttgga
     5341 caggtcgagg aatggccgcg tctcgcggcc atgcggcctg caaggcccac cagcggaccg
     5401 gcaaagccgg ttccgcggtg gcccccgcac tgaccgcagg gtttcatgcg ctgcggctgg
     5461 cgcgaagcgc aatggactac tgacactaca tccaaaggag cctcacatga gctttccagg
     5521 cctgaacttc cagctcggcg aagacatcga cgcgttgcgc gacgccgtgc gcggctttgc
     5581 ccaagccgag attgcgccgc gcgcggccga gatcgaccgc agcgaccagt tcccgatgga
     5641 cctgtggcgc aagatgggcg agatcggcgt gctcgggatc accgtgcccg aggagtacgg
     5701 cggcgccaac atgggctacc tcgcccatat gatcgcgatg gaggaaatct cgcgcgcctc
     5761 ggcctcggtt gccctctcgt atggcgccca tagcaacctg tgcgtgaacc agatcaagcg
     5821 caacggcaac gaagcgcagc gccgcaagta ccttcccaag ctgatctcgg gcgagcatgt
     5881 cggtgccctg gccatgagcg aggcgggcgc cggcagcgac gtgatcggca tgaagctcaa
     5941 ggccgaggac aaagggggcc attacctcct gaacggcagc aagatgtgga tcacgaacgg
     6001 accggatgcc gacacgctcg tcgtctatgc caagaccgag ccggaactgg gcgcgcgcgg
     6061 cgtgacggcc ttcctgatcg aaaagggcat gaagggcttc tcgatcgcgc agaagctcga
     6121 caagctgggc atgcgcggct cgcataccgg cgagctggtg ttccagaacg tcgaagtgcc
     6181 cgccgccaat gtgctgggca acctcaacgg cggcgcgaaa gtcctgatga gcggcctgga
     6241 ctacgagcgc gcggtgcttg ccgccggccc gatcggcatc atgcaggccg tgatggacaa
     6301 cgtcgtgccg tacatccacg accgcaagca gttcggccaa agcatcggcg aattccagat
     6361 gatccagggc aaggtggccg atatgtacac cgtgctgcaa gcgggccgcg ctttcctgta
     6421 caccgtgggc aagaacctgg acatgctggg cgccgaacat gtgcggcagg tgcgcaagga
     6481 ctgcgcctcg gtgatcctgt ggtgcgccga gaaagcgacc tggatggccg gcgagggcat
     6541 ccagatcttc ggcggcaacg gctatatcaa cgagtatccg ctgggacggc tgtggcgcga
     6601 cgccaagctg tatgagatcg gcgcgggcac cagcgagatc cgccgcatgc tgatcggccg
     6661 cgagctgttc gccgaaacga tgtgattata tttggcatcc ctaaacatta cctgtcatcc
     6721 cgggcttgac ccgggatcca tggtggcgcg ccacggtgga ttgcggggcc gggccggagc
     6781 gtgatgcctc ggtacgtgca tagcgcaggc ccggcattgg gctcgctaca ctggacagct
     6841 gcgcgccccg ggcgttccgc gagcccagcc cgcaatgaca agttcagggg catccgcaat
     6901 ttcacgtagt tcccgacaag tagccccggc aagcgaaaat accgctcatg acgtcctcct
     6961 cgctcaacga cctgttcgtc cacaaccggg aatgggccgc ccgaatggag cgcgagcggc
     7021 ccggcttctt cagcggcttg acccagcagc aacagccccg ttacatgtgg atcggctgtt
     7081 ccgacagccg ggtgcccgcc aaccagatca ccggcctgga gccgggcgag gtgttcgtgc
     7141 accggaacgt ggccaacgtg gtggtaccca cggacctgaa ctgcctgtcg acgatccagt
     7201 tcgcggtcga ccagctcaag atccggcacc tgatggtggt cgggcactac ggctgcggcg
     7261 gcgtgcgcgc cgcgctggaa ggggtccgca tcggcctggc cgataactgg ctgcagcatg
     7321 tgaaggatgt ccgtgaaaaa cacagcgggt tgctggactg ggtgccgcag gaccgccggc
     7381 tcgatgcgct gtgcgagctg aacgtgatcg agcaggtcgc gaacgtggcg cataccaccg
     7441 tcatgcagga tgcctggcag cgcggccagc gcgtgacact gcatggctgg gtgtatggcc
     7501 tgtcggacgg gctgctcaag aacctgaaca tcagcgtcga gaacaatgaa cacctgggcc
     7561 ccgcctaccg cgaggcggtg acggccgtgg cctgcgcccg gcggccctag cgcgaggccc
     7621 cactttgttt ccccagcgcc tcgattcccc attggcctac ggcatcgcca aggcgatgat
     7681 ggacggcttc aaccggcatt accggctgtt ccgcaccgag tcggcgcggg ccaagcaccg
     7741 gttcgaaacg gccgactggc acggccagca gcgctcccag cgcgagcgca tcgagttcta
     7801 cgaccttcgc gtcaaggaag cggtcacccg cctggagaag gaattcaagg ccggcgagca
     7861 gccgatggaa gtctggcagc aggtgaagct gcactacatc gggctgctgg tggaccacca
     7921 ccagccggag ctggccgaaa ccttcttcaa ctcggtcacc accaagatcc tgcaccgcac
     7981 ccacttccac aacgacttca tcttcgtgcg gccggcggtg agcaccgaat acatcgagaa
     8041 cgacgagccg gccgccatgc cgacgtatcg ctcttattac ccgacgcgcg aaaacctgtg
     8101 cgacaccatt atccgcatca tcgacaactt ccggctgcag caccccttcg atgacctgcc
     8161 gcgcgacgcc cgctacgtgc tggaggccat cagcgcgcgg ctgcagcacg tcaagctgcg
     8221 ggccaatttc cagatccagg tgctgtcctc cctgttctat cgcaacaagg gcgcgtacgt
     8281 ggtcggcaag atcatcaacg gcttcaacga ggtgccgttc gcactgccga tcctgcacaa
     8341 ccgctccggc aggctggtca tcgacacctg cctgttcggc gaggacgaca tgcagatgct
     8401 gttttccttc gcccgcgcct acttcatggt cgacatggag gtgccctcgg cctacgtgca
     8461 gttcctgagg tcgctgatgc cgcgcaagcc ccgggccgag atctacaacg cgctcgggct
     8521 ggccaagcag ggcaagacgc ttttctaccg cgacttcctc taccacctga agcattccag
     8581 cgacaagttc cgcatcgcgc ccggtatcaa gggcatggtg atgctggtgt tcgacctgcc
     8641 gtcctttccc tatgtgttca agctcatcaa ggactattac ccgccgcaga aggagaccac
     8701 gcgcgagcag atcaaggcca agtacctgct ggtcaagcag cacgaccggg tgggccgcat
     8761 ggccgatacg ctggagtaca gcgaagtcgc cttcgcccgc gagcgcttcg aggacgagct
     8821 gatcgaggaa atccgcacgc atgcccccag ccagctccag atcagcgccc gccgcaatga
     8881 ggatggaagc gacggccacg aagaggtcat catcaagcac ctttacatcg agcgccggat
     8941 gatcccgctg aacatctacc tgcaggaagc cttcgacgcc tggcatgacg agggccagga
     9001 cagcctggag gccgggcatg cccacgacat gatcgagcgc gcggtgatcg aatacggcaa
     9061 cgccatcaag gacctggtgt ccgccaacat ctttcccggc gacatgctgt tcaagaactt
     9121 cggtgtcacg cgcaacggca aggtggtgtt ctacgactac gacgagatcg aatacatcac
     9181 ggactgcacc ttccgcaaga tcccggacgc gcgcaccgaa gaagaggagc tgtcggccga
     9241 agtctggtac cgggtcggcc ccaaggacgt ctttcccgaa accttcgccc cgttcctgct
     9301 gggcaacccg gccgtgcgcg aggtgttcat gaagcaccac gcggacctgc tggaggcttc
     9361 gttctggcag agccacaagg agcgcatcca ggccggctac gtgcatgacg tgtttcccta
     9421 cgaacgcgag aagcggtttt cccggcggta cccccccgcc gccggcggcg cgccatcgca
     9481 gagcccttcc gccgtgggga agtggccctc ccgggacacc cgtcccggcc gcggagaagg
     9541 cagggtgacg ggcacatgaa cgccagagac gcctgggagt acgcgagctt tgtcgttaca
     9601 gcgctcgcgc tgccgttcgc tattgccttc ttcgcctggg agcagcgcaa ggagcgcgac
     9661 aacgaagagg aagagggtta ccagctgctg tcgaacgcct acaacgactt cctcaaggtg
     9721 gtgctggcca acccggacct gcagctgcga accaacgagc cgctgtcgaa tccaacgccg
     9781 gagcaaaatg agcggatgct cgtgattttc gacatgctga tgtccctgtt cgagagggcc
     9841 tatcttgtgg cctacaagcc caagatgagc gaaacggagg cccggcgctg gaacagctgg
     9901 gacgactaca tgcgcgaatg gtgccgtcgg gaggatttcc acaacgccct gccgctgctt
     9961 ttgcgcggcg aggaccctga gttccagagt tatttacgcc gcattgccca agaggaacgc
    10021 ggcacattga ttcaactaag ctgaaaggag cttgccatgt ctgattccat cgttatcgtc
    10081 ggttccgccc gcacccccat gggcgctttc cagggcgatt tttccgcgct gtcggcgcac
    10141 gacctgggcg gcgccgcgat caaggccgcg gtcgagcgcg ccgggatttc cggcgacgcc
    10201 gtgggcgagg tgctgttcgg caactgcctg atggccggcc agggccaggc gccggcgcgc
    10261 caggcggcat tcaagggcgg cttgcccaag agcgcgggcg ccgtcacgct gtccaagatg
    10321 tgcggttcgg gcatgaaggc ggcgatgttc gcccacgaca tgctgctggc cggcacgcac
    10381 gaagtgatgg tgacgggcgg catggaaagc atgaccaacg ccccctacct catgctcaag
    10441 gggcgcagcg gctaccgcat gggccacgac cgcatcttcg accacatgat gctggacggg
    10501 ctggaagacg cctacgaggc cggccgttcc atgggcacct tcggcgagga ctgcgcggcc
    10561 aagtacaagt tcacgcgcga agcgcaggac gcgttcgcga tcgccagcgt caagcgcgcc
    10621 aaggccgcga ccgagtcggg tgcctttgcc accgagatca cgcccgtcac ggtgaagagc
    10681 cgcgccggcg aggtggtggt ttccatcgac gaaggcccgg gcaaggtcaa gctcgacaag
    10741 attcccacgc tcaagccggc gttcaagaag gacggcacca tcacggccgc gtccagctcc
    10801 agcatcaacg acggcgccgc ggcgatggtg ctgatgaagg agtccaccgc cgtgcgttac
    10861 ggcagcaagc cgatcgcccg catcgtgggc cacgccatgc actcgcagga g
//
LOCUS       DASWIY010000025        18375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_759212, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000025 DASWIY010000000
VERSION     DASWIY010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 18375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18375
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759212"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VGA59_00865"
     CDS             <1..356
                     /locus_tag="VGA59_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HEX9718256.1"
                     /translation="SDIVARMALVPRTLEARGLDATPLIQEKLRRVGTPAARRAVDIL
                     DILLRDEIGHVAIGNRWYRWLCQREGLDPVAHYGVLVERYEAPRLYPPFNEEARRQAG
                     FSTEELALMGRPGPG"
     gene            complement(426..2147)
                     /locus_tag="VGA59_00870"
     CDS             complement(426..2147)
                     /locus_tag="VGA59_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9718257.1"
                     /translation="MFQKSTITTRLLSGFALVFALLVVLVASGLAYFNSLTDRIDLLA
                     NNRLPQVIAAGQWEASVLRTARHMQAALLLTEPGERQQELRSIKDNRDEQAALHARVK
                     ELAAASEAQTLLQRIDRARRTYLQAEAEFVQQAEAGKLDEAKVVLLVKTRPAQAAYIG
                     GISKLGSFLSDEAREMSRTSAAAYRISIGAMILAALLCLAGAYAVTLMISRSLQKQLG
                     GEPAYARSVVEQVAGGDLTVEVALQKGDESSLLAAMREMISKLRSMVGETARGARSVA
                     DTSAQIAQGNLDLSQRTEEQASTLEEAASSMQELTSTVAQNAQHARQANELAAGASDT
                     ARKGGEVVDEVVNTMDQILDASRKIADIVGVIDAIAFQTNILALNAAVEAARAGEQGR
                     GFAVVATEVRNLAQRTTLAAKEIKALISDSNQKVQAGSSKVDAAGHTMVGVVMSVQKV
                     SNLIAEIAAASQEQSSRIGQVSSAVKQMDTVVQQNASLVEEAAAATESMKDQAGALLE
                     LVSRFRLGEGDESFSSTGASVFAAPRPAASPGPIRVRPRRGVPPQYIPPVGLDHDGRS
                     NRKPSLN"
     gene            complement(2164..3243)
                     /locus_tag="VGA59_00875"
     CDS             complement(2164..3243)
                     /locus_tag="VGA59_00875"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014722236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX9718258.1"
                     /translation="MIRVLVVDDSAVMRAFLGQVVASQPDMELAGVVSDAVLAIDRIR
                     RDAPDVITLDVEMPRMNGLDFLRQLMVARPLPVVMISSLTLKGADATIRALELGAVDY
                     FAKPASFEEFHGAAPEIAEKIRAAAQARVFPRRRSSAGRPAAAPRLAGLGRPGASGTI
                     IGIGASTGGVEGLRVMLTELPRDMPPILIAQHMLPGFTGAFARRLDDVCEMSVKEAED
                     GEEALAGVAYVAPGGRHLLLARRRAGYVLRLSDDPPLNRHRPSVDALFRSMAETAGAR
                     AIAVILTGMGSDGAQALLELRNAGARTLAQDEASSVVFGMPRQAIALGAAQEVLALGD
                     MPRRLKELCGHEPLPAAADLGSNGD"
     gene            complement(3240..3845)
                     /gene="cheD"
                     /locus_tag="VGA59_00880"
     CDS             complement(3240..3845)
                     /gene="cheD"
                     /locus_tag="VGA59_00880"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF010013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HEX9718259.1"
                     /translation="MEHPAPTRYFDREFQIASVKIQPGQYYAAGGEGSICTVVGSCVS
                     TCLWDPVRRIGGMNHFMLPGEPSGTASPWGLSARFGVYAMEVLINEMLHLGAERGRLV
                     AKVFGGAQVLQGFDTLDVGRMNSTFVLNFLQEEGIQLLAQDLLGTCPRKLHFFPASGK
                     VRLKKLHLQQYDVVQQQEREYLVRLGETAGGGEIEIFSAPP"
     gene            complement(3860..4678)
                     /locus_tag="VGA59_00885"
     CDS             complement(3860..4678)
                     /locus_tag="VGA59_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX9718260.1"
                     /translation="MSDEGSGLLGEQEFALACRLIGEYAGIKLSGHKRYMVHNRLTRR
                     LRACGLASFADYLRLVQNEQSGEREAFVNALTTNLTAFFREPHHFELLRSHAQLHRER
                     ERRPMRVWCCACSTGEEAWSIAMTLREAACPGDILATDIDTEALEAASAGVYPLERAS
                     AIAPERLRSHLLRGTGGNSGWVSVRPELRSMVRFMRFNLQLPQWPAMEPFDAIFCRNV
                     VIYFDQEAQKQLLARLAASLRPRGLLAVGHAESFPASHRSFRACGRTAYEYVPA"
     gene            complement(4681..6372)
                     /locus_tag="VGA59_00890"
     CDS             complement(4681..6372)
                     /locus_tag="VGA59_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9718261.1"
                     /translation="MKMKIQLAADSLLNMMFAGSLVGVIAVGALGYYALEESHERTVA
                     ALQSGRELGAAAALVRAADTAAHAQATEVDQLLAMRAPEAAGAAPRPGLMRKSDEVVR
                     KHMDDARQLLTKHGLDAARLEDAGSLQAKLTQQAELRLKHAAAGEQALPHAEKWADVA
                     PGVEAVVQRQTQQIAQDSADLTRNMGLIVAFVVLSGMAAAMVLRPMILRANVRPLISA
                     IKLVEQIAAGDLAAKPVGMDEPHTRRLARALEGMTDSLRTLALEVVRSARAVADTSGQ
                     IAHGNLDLSQRTEEQASTLEETASSMEELTSTVAHNADNAREASHLAVGASDVARKGG
                     EVVGQVVATMTDISQSSRKIGDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVV
                     ASEVRNLAQRSAAAAKEIKTLIGESVIKVEAGTKLVDSAGKTMDEIVASVKKVKDLIS
                     EIAAASQEQSAGIGQVNTAVAQMEQVVQQNASLVEEATAATESMKEQAASLLRMVERF
                     KVGEQAAAITALQPATLWPAAPAPIKVKAATALPPAYATAASHKAKAGNGEWEEF"
     gene            complement(6417..6932)
                     /locus_tag="VGA59_00895"
     CDS             complement(6417..6932)
                     /locus_tag="VGA59_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX9718262.1"
                     /translation="MQQQGYRTTSQPGDSRNAEARKSEFLTFRLGSESYGIEILKVQE
                     IRGYETPTSIANAPPFIKGVINLRGVIVPILDLRVKFRLPDTPYNEFTVVIILKVASR
                     VVGIVVDSVSDVVSLDAEAIRPTPEFASATFDTKYITGLATVDGGMLIMLDIERLLTG
                     ADMALVESAME"
     gene            complement(6949..9009)
                     /locus_tag="VGA59_00900"
     CDS             complement(6949..9009)
                     /locus_tag="VGA59_00900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX9718263.1"
                     /translation="MYNLTGALGDPNAYRAIFFEETQDHLANVESILLRLAPEQPCLD
                     DLNAIFRAVHSIKGSAAMLGCSDMAALTHLQESLLDLLRKEERPLEAGDVEAMLKAGD
                     AVRAQALCHRGVLDQPPDGSAVEAMLREQLARPRSDARQAASGPVVRRFAVRLAPLEE
                     PIAKSELDMMLGGLSEMGTVTDTVIENVPGGCVSFDVQLEGTAADLRSVLSLLVAPEL
                     ISIEKTGGGESATLAPAAFGAAAALHAHDEFFVDPEEFRRKRQALAQETGQAPDPGPE
                     TIGPAEPSNTDRPSAQAAEQIVMQVPDTGHIRVATEKIDLLVNLVGELVITEAMLARC
                     GRLDDSAAGGRLEGGLADLARHTRNLQEAVLAIRMLPISNAFSRFPRFAHELASRLGK
                     RVELQFEGETTEVDRGLIEKVSDPLTHLVRNAIDHGLETPEVREAAGKPAAGSLRLQA
                     RQRGGTIVIEVSDDGCGLNRERILAQAARCGIAIAPDAPDAQVWELVFAPGLSTAEEV
                     TDLSGRGVGMDVVRRNIKALGGTVDISSTAGRGTTFTVRVPLTLAIIDAMIVAAGDAT
                     YVLPLGAVMESLSVEPGEIHALPGQGDTLRVRDAYLPVLRLAEIFPPRNASEQPGGIA
                     VIVEADGTYAALLVDEVVGQQQVVVKSLEANFRKVPGLAGATVMGDGSVALILDVAHI
                     VRMS"
     gene            complement(9022..9384)
                     /locus_tag="VGA59_00905"
     CDS             complement(9022..9384)
                     /locus_tag="VGA59_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9718264.1"
                     /translation="MNKAILVVDDTRSMRKMVGAVLAGAGYDVAEAGDGAEALELARA
                     RQFDLVVTDHNMPRMDGVTLVRALRQLSNYDAVALIVLSTEVDPVLKQQGREAGATGW
                     MAKPFDPQRMLDIVAKFI"
     gene            complement(9386..10270)
                     /locus_tag="VGA59_00910"
     CDS             complement(9386..10270)
                     /locus_tag="VGA59_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9718265.1"
                     /translation="MNQNDQYLVDVIGFNDVERSMLSSIFSLAARRDPGFAQYEPGEA
                     GRKPDVYLVDADNPAAFGEFRALHRRARLPAVLVGAGPQGTNCLVIPRPLQWARLLQA
                     LDDTLAGGGQEPPVSTATAAPQTPQGLRRSLSGASLARSNSLREMPAAEATKRVLGDT
                     VLVVDDNATVRMFMQAKLAPFGFEVDIAETGEEAIGLSATQEYTCVFLDVVLPGIDGY
                     QVCKLIKSNKQAIKKTAVVMLTSRSSPFDKLRGSLAGCDEYLTKPLDEDRLLEVIAKF
                     LPSSRKHHHHHRERASRK"
     gene            complement(10267..11208)
                     /locus_tag="VGA59_00915"
     CDS             complement(10267..11208)
                     /locus_tag="VGA59_00915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9718266.1"
                     /translation="MSALAAVLIRPRSRSGPADHAASPRRQDAGALGLAHSLAGYRLE
                     AVLGRGRHSIVYLARQPQGAQIVALKVGRETRLEGMDAKQDFGGEFGVLSALAHSHVI
                     RVFDHGESDGEAYLAMEYAAGGRMAPYSAPVSAARMVTRLREAASALGWLHTNGWVHR
                     DVKPANLLLRADGSLALGDFGCACRRGNAGALPPGTVVGTPRYAAPEQSAGAPAQPTA
                     DVYSLGVCLYEMLTGKPLYPGRTLAELLGQHLLAAIPRLARDQAAWQPLLDAMLAKDP
                     AQRPADGRAVLALLERAGRVLWQPHGRGSSHESRNPS"
     gene            complement(11205..12845)
                     /locus_tag="VGA59_00920"
     CDS             complement(11205..12845)
                     /locus_tag="VGA59_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9718267.1"
                     /translation="MGGETSQDANAHFAPTDVAPLESLFGASSVSPYESARPEPEASA
                     EAEGTLATFMYARLRNFAVTCETMSASELTSFVNEVRRALSSAGLKLGGEIAQRRPDS
                     ILCVFSHKPEDRLPVHAKRALHAAILTVHEFVQLATRIAARPQSSALPPLSIAVGVHL
                     GAGEVVPRATNSHGMVHATGEAVEIARLLPIVAADLHWGVVTSLATRQAAGTRVESGR
                     HATFGLPDDTFLEVVEISGLVPRKGSVTPPSHYEALRNSLERNQQLARAAANAAAAAN
                     PQAVQGPGHVLIEGYRLLRKIGEGGNATVYLAQPMVGGPTQVLKVLRPDREGEMDLQR
                     FIQEFALLAQTDHPNVARIFRQDFSGGSGYIAMEYFPLGDLRSRMRQALDPGIALYYL
                     KQIAAGLEAIHQVGIVHRDLKPDNVMIRQDGMLAIADFGVAKQVSMEITDTGAGDIIG
                     TPYYLSPEQVLGKPVDARCDLYSLGIVAYEMFTGMKPYHAETARALLDLHVNAPVPAL
                     PAALQRFQPVLEKMMAKDPAQRYASATALLDALEELAQ"
     gene            complement(13201..14085)
                     /gene="xerD"
                     /locus_tag="VGA59_00925"
     CDS             complement(13201..14085)
                     /gene="xerD"
                     /locus_tag="VGA59_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX9718268.1"
                     /translation="MSNASIDAFIDALWLEDGLSRNTLAAYRRDLSLYAGWLAELGRT
                     IDSTGEGDLNGYFAARHAQSKATTANRRLTVFKRYFRWALRERLIAADPTLRLQSARQ
                     PLRVPKSLTESQVEALLDAPEVDKPLGLRDRTMLELMYASGLRVSELVSLKTFNVSMG
                     EGVLRVLGKGSKERLVPFGLVARDWITRYLTEARPVILGGQQSGDLFVTGRGEGMTRV
                     MFWMIVRKYARSAAITAPLSPHTLRHAFATHLLNHGADLRAVQMLLGHADISTTTIYT
                     HVARERLKQLHAAHHPRG"
     gene            complement(14207..15181)
                     /locus_tag="VGA59_00930"
     CDS             complement(14207..15181)
                     /locus_tag="VGA59_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugE"
                     /protein_id="HEX9718269.1"
                     /translation="MKQRRLWFVLPAAALALAAGPALAQGYPNKPIKLQVPFAPGGTT
                     DIIARVIADPLGKALGQPVIVENRAGGGGVIGALETVRSAPDGYLLGMATVSTTAANP
                     AINPRTPYNPTTDFTPIINIAATPNVIAVHPSFPAKTYLAFVAELKRSPGRYSFASSG
                     TGGIGHLQMELYKSLSGTFITHIPYRGAGPALNDTVAGQVPIIFDNLPSAQPFIKDGR
                     LVPMVVAAPQRLASLPNVPTFKEVGLEPVNRMAYYGILGPKGMPKEVVAKIHAATRKV
                     LEDPAVRKRIEETGSLIVGNTPQQFAEQIRAEYAVYKSVVESAKLKLE"
     gene            complement(15318..16415)
                     /gene="queG"
                     /locus_tag="VGA59_00935"
     CDS             complement(15318..16415)
                     /gene="queG"
                     /locus_tag="VGA59_00935"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX9718270.1"
                     /translation="MFNSGQIDGPRLVGQIREWALELGFSQIGVAGVDLSSAEPGLLA
                     WLSNGFHGEMAYMQTHGLKRARPAELVPGTASVITARMDYLPAATPEGWQAVEFDRLR
                     RPGEGAVSVYARGRDYHKVLRSRLRKLSDRIASRIGPFGHRVFTDSAPVLEAELAARS
                     GQGWRGKHTLVLNREAGSMFFLGEIYVDMKLPVSDPARGHCGTCSACIEVCPTQAIVA
                     PYRLDARRCISYLTIEHAGPIPEELRPLIGNRIYGCDDCQLICPWNKFARRSALPDFD
                     ERGGLTARQLVELFGWSEEEFLRHTQGGPIRRIGHERWLRNIAVAIGNALRVREDPLL
                     RAALQTRADHPSALVREHVAWASAQESTQQP"
     gene            16455..16922
                     /gene="tsaE"
                     /locus_tag="VGA59_00940"
     CDS             16455..16922
                     /gene="tsaE"
                     /locus_tag="VGA59_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX9718271.1"
                     /translation="MGAPLDAAVVVWRNEDDTRAFAAKLAAHPAIGGAFIELHGDLGA
                     GKTTLARHLLRALGVQGRVKSPTYTVLEPHEARGLAIWHFDFFRFNDPREWEDAGFRD
                     IFAGPGLKLAEWPEKAAGLPPRADLVVRIEAQADESRNVTLLAQTPLGRELLE"
     gene            16919..18229
                     /locus_tag="VGA59_00945"
     CDS             16919..18229
                     /locus_tag="VGA59_00945"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9718272.1"
                     /translation="MKRRELLRRGTLVLLLGTRQIARGATIVAVRVWPAPDYSRVTIE
                     SDRQLSSTQIVMANPPRLAVDIMGIDLIPELRELVAKVKADDPFIEGIRVGQQSPGVV
                     RLVVDLKQASVPQVFTLTPVAAYQHRLVFDFYPLKTVDPLEALIAERLKDQPVAPPTP
                     ASDPLGELIAQRLEGDRRSGVIASAEAAPRPAPQKTDRLIIIALDPGHGGEDPGAVGR
                     AGTREKDVVLQIAHRLRERINASVINGNPMRAFLTRDADFFVPLHVRVQKARRVQADL
                     FVSIHADAFLTPTARGASVFALSRGGASSTAARWIADRENRADLIGGVNVKARDAHVA
                     HALLDMSTTAQINDSLKLGSALLGEIGNVGRLHKGQVEQASFAVLKAPDIPSVLVETA
                     FISNPEEEARLRSDQYQEQLADALMRGIHRYFAKNPPLARSRAL"
BASE COUNT         2959 a         6318 c         6041 g         3057 t
ORIGIN      
        1 gcagcgacat cgtcgcgcgc atggcgctgg taccgcgcac gctggaagcg cgcggcctgg
       61 acgcgacgcc gctgatccag gagaaactgc gcagggtcgg cacgcccgcg gcgcgtcgcg
      121 cggtggacat cctggacatc ctcctgcgcg acgagatcgg ccatgtggcc atcggcaacc
      181 gctggtaccg ctggctgtgc cagcgcgaag ggctggaccc cgtggcgcac tacggtgtcc
      241 tggtcgagcg ctacgaggcg ccccggctgt atccgccgtt caacgaagaa gcgcgaagac
      301 aggcaggctt cagcaccgag gagcttgcct tgatgggaag acccgggccc ggctaaaccg
      361 cagcttcgcg ggcaaaggcg ctgctggcgg ccccgcggcg aaggccgtcg gcgcgagcac
      421 cgacactaat tcaagctggg cttgcggttc gaacgcccgt catggtccaa accgaccggc
      481 ggaatgtact ggggcgggac gcccctgcgg ggccgcaccc tgatggggcc ggggcttgcg
      541 gcaggacgcg gcgccgcgaa cactgacgca cccgtggaag aaaacgactc gtcgccctcg
      601 cccagacgga agcgcgacac cagttccagc agcgcgcccg cctggtcctt catcgactcc
      661 gtggcggcgg ccgcttcctc gacgagcgac gcgttctgct gcaccacggt gtccatctgc
      721 ttgaccgcgg agctcacctg cccgatcctg gaactctgct cctggctggc cgccgcgatc
      781 tcggcgatga gattgctgac cttctgcacc gacatgacca cgcccaccat cgtgtgcccc
      841 gcggcatcaa ccttgctgga gccggcctgg accttttgat tcgagtcgga gatcaaggcc
      901 ttgatctcct tggcggccag ggtggtgcgc tgggcaaggt tgcgcacttc ggtggccacc
      961 accgcgaagc cccgcccctg ttcacccgcg cgggcggctt ccaccgccgc attgagcgcc
     1021 aggatgttcg tctggaaggc gatggcgtcg atcacgccca cgatgtcggc aatcttcctg
     1081 gaggcgtcca gtatctggtc catcgtgttg acgacctcat cgacgacctc accgcccttg
     1141 cgcgccgtat cggatgcgcc cgccgcgagc tcattggcct ggcgggcatg ctgggcgttc
     1201 tgggcgacgg tggaggtcag ctcctgcatc gaactggctg cttcttccag ggtgctcgcc
     1261 tgctcttcgg tccgctgtga caagtccagg ttgccttgcg cgatctgggc actggtgtcg
     1321 gccacggatc gcgcgcccct cgctgtttct cccaccatcg accgcagctt cgagatcatt
     1381 tcacgcatgg cggccagcag actgctttca tcaccctttt gcaatgcgac ttccaccgtg
     1441 aggtccccgc cggccacttg ctcgaccacc gatcgcgcat aggccggctc cccgcccaat
     1501 tgcttttgca agctgcgcga gatcatgagc gtcacggcgt aggccccagc caggcagagc
     1561 aaggcagcaa ggatcatggc cccgatactg atgcggtacg cggcggccga tgtccgcgac
     1621 atctcgcgcg cttcatcgga gaggaatgag cccagcttgg aaatcccgcc gatgtacgcg
     1681 gcttgcgcgg gccgggtctt gaccagcagc acgaccttgg cttcgtccaa ctttcccgcc
     1741 tcggcctgct gcacgaattc cgcctcggct tgcaggtacg tcctgcgggc ccggtcgatc
     1801 cgctgcaata aggtctgggc ctcgctggcg gccgcgagtt ccttgacacg cgcgtgcagc
     1861 gccgcctgct cgtcgcggtt gtccttgatg gacctcagtt cctgttgccg ttcacccggt
     1921 tcggtgagaa gcagcgccgc ctgcatgtgc cgggcggttc tcagcaccga cgcttcccat
     1981 tgaccggcgg cgatcacctg cggcagccgg ttattggcca gcagatcgat ccggtcggtc
     2041 agcgaattga aataggccaa gcccgatgcc accagcacaa cgagcaaagc gaacacaagc
     2101 gcgaaaccgc tcagaaggcg cgtggtgatg gtggatttct ggaacatgaa atgtaacaat
     2161 ttgctaatct ccattagacc ccaaatccgc ggcggcgggc aggggctcgt gcccgcaaag
     2221 ctccttcagt cgcctcggca tgtcgcccag cgccaacacc tcttgcgcgg cgcccagcgc
     2281 aatcgcctgc ctgggcatgc cgaacaccac gctgctggcc tcgtcctggg ccagggttct
     2341 cgccccggcg ttgcgcagct ccagcagggc ttgggccccg tccgacccca tgcccgtgag
     2401 aatcacggca attgcgcggg cgccggccgt ctcggccatg gagcgaaaca gcgcgtcgac
     2461 cgaagggcga tgccggttca gtggcggatc gtcgctcaac cgcagcacgt accccgcccg
     2521 gcggcgcgcg agcagcaggt gccgcccgcc cggcgcgaca tacgcgacgc cggcaagcgc
     2581 ctcctcgccg tcttcggctt ccttgaccga catctcgcaa acgtcgtcca gccgccgcgc
     2641 aaacgccccg gtgaagcctg gaagcatgtg ttgggcaatc aggatcggcg gcatgtcgcg
     2701 gggcagctcg gtcaacatca cgcgcaatcc ctcgacgccg cccgtggatg caccgatgcc
     2761 gatgatggtt cccgacgcac cgggtcggcc gaggccggcc agcctcggcg ccgccgcggg
     2821 ccgcccggcg ctggatctgc gtcgcgggaa cacgcgcgcc tgcgccgcag cccggatctt
     2881 ctcggcgatc tcgggcgcgg cgccatggaa ctcctcgaac gaggccggct tggcgaaata
     2941 gtccaccgct cccagctcga gcgcccggat cgtcgcgtcg gcacctttga gcgtcagcga
     3001 ggaaatcatg accaccggca gcggccgcgc caccatgagc tggcgcagga aatccagccc
     3061 gttcatccgc ggcatctcga cgtcgagcgt gatgacgtcc ggcgcgtccc ggcgaatgcg
     3121 gtcgatggcg agcacggcgt cggaaacgac gcccgccaat tccatgtcgg gctgcgacgc
     3181 gaccacctgc ccgaggaagg cgcgcatcac ggcggagtcg tcgacaacca gcaccctgat
     3241 catggaggcg ccgagaagat ttcgatttcg ccgcccccgg cggtctcccc caggcgcacc
     3301 aggtactcgc gctcctgttg ctggacgacg tcgtactgct gcaggtgaag cttcttgagc
     3361 cgcaccttgc cgctggcggg aaagaagtgc agcttgcggg ggcaagtgcc cagcaggtcc
     3421 tgcgccagca gctggatgcc ctcctcctgc aggaagttca gcacgaaagt gctgttcatc
     3481 cggccgacat ccagggtatc gaacccctgc aggacctgcg cgcccccgaa gaccttggcc
     3541 accaggcggc cgcgctcggc gcccaggtga agcatctcgt tgatcagtac ctccatggca
     3601 tagacgccga atcgcgcgga gagcccccag ggtgacgccg ttcccgaggg ctcgcccggc
     3661 agcatgaaat gattcattcc gccgatgcgc cgcaccgggt cccacaggca ggtggaaacg
     3721 cacgagccaa cgacggtgca gatcgagccc tccccgccgg cggcgtagta ctggcccggc
     3781 tggatcttca ccgacgcgat ctggaactcg cggtcgaaat agcgggtcgg ggcgggatgt
     3841 tccatcgcgg cgcggtccgt caggccggga cgtactcgta cgcggtgcgc ccgcatgccc
     3901 ggaacgaccg gtgcgacgcg ggaaaacttt ccgcatggcc caccgccagc agtccgcgcg
     3961 ggcgcagcga cgcggccaat cgcgccagca gctgcttctg tgcctcctgg tcgaagtaga
     4021 tcaccacgtt acggcagaag atggcgtcga agggctccat cgccggccac tgcggcaact
     4081 gcaggttgaa ccgcatgaac cgcaccatcg agcgcagttc cgggcgaacg ctcacccagc
     4141 ccgagttgcc gccggtgccc cgcagcaagt gagagcgcag gcgctcgggc gcgatggcgc
     4201 tcgcccgctc gagggggtac acacccgcgc tggccgcctc cagcgcctcg gtgtcgatat
     4261 cggtggccag aatgtccccg gggcacgcgg cctcgcgcag cgtcatggca atggaccagg
     4321 cttcctcgcc ggtcgaacag gcgcagcacc acacgcgcat cgggcgccgc tcccgctcgc
     4381 gatgaagctg cgcatggctg cgcagcagct cgaagtggtg gggctcgcga aagaacgccg
     4441 tcaggttggt ggtcagggca ttgacgaatg cttcgcgctc gcccgactgc tcgttctgca
     4501 ccaggcgcag gtaatccgcg aagctcgcca ggccgcatgc gcgcaagcgc cgggtcaggc
     4561 gattgtggac catgtagcgc ttgtggccgc tgagcttgat gccggcgtat tcgccgatca
     4621 ggcggcaggc gagcgcgaac tcctgttcac ccagcagccc cgagccttca tcggacatgc
     4681 ctagaattct tcccattcac cgttgccggc ctttgccttg tgcgaagcgg ccgtggcata
     4741 cgcaggcggc agcgccgtgg cggccttcac cttgatcggc gccggcgcgg cgggccacag
     4801 ggtcgcgggc tgcaacgcgg tgatcgccgc ggcctgctcg cccaccttga aacgctcgac
     4861 catgcgcagc agcgatgcgg cctgctcctt catggattcc gtggccgcgg tcgcttcttc
     4921 caccagggat gcattctgct ggaccacctg ctccatctgc gccaccgcgg tgttgacctg
     4981 gccgatgccc gcgctttgct cctggcttgc cgccgcgatc tcggagatca ggtccttgac
     5041 cttcttcacc gaggcaacga tctcgtccat cgtcttgccc gcggagtcca ccagcttggt
     5101 gcccgcctcc accttgatca cggactcccc gatcagcgtc ttgatctcct tggccgccgc
     5161 ggcgctgcgc tgcgcgagat tgcgcacctc cgaagccacc accgcgaagc cgcgcccctg
     5221 ctcgccggcg cgcgcggctt ccaccgcggc attcagagcc aggatgttgg tctggaacgc
     5281 gatcccatcg atcacaccga tgatgtcgcc gatcttgcgc gacgactggg agatgtccgt
     5341 catcgtggcc accacctggc ccacgacctc gccgcccttg cgcgcgacgt ccgaggcgcc
     5401 cactgcaaga tggctggcct cgcgcgcgtt gtccgcgttg tgggcgactg tcgaagtgag
     5461 ctcctccatc gagctggccg tctcctccag cgtgctggcc tgctcctcgg tgcgctgcga
     5521 caggtccagg ttgccgtgcg cgatctggcc gctggtgtcg gccaccgccc tggcgctgcg
     5581 aacgacctcc agcgcgagcg tgcgcaggct gtcggtcatg ccttccagcg cacgggccaa
     5641 tctgcgggta tggggctcgt ccattcccac cggcttggca gccagatcgc ccgcggcgat
     5701 ctgctcgacc agcttgatcg ccgagatcag cgggcgcacg ttggcccgaa gaatcatcgg
     5761 gcgcaatacc atagcggccg ccatgcccga aaggacgacg aacgcaacga tcagccccat
     5821 attgcgcgtg aggtcagccg agtcctgcgc gatctgttgc gtttgccgct gcacaacggc
     5881 ttcgacgcct ggcgcgacat cggcccattt ttccgcgtgc ggcagcgcct gttcgcccgc
     5941 agcggcatgc ttcagccgca gttcggcctg ttgcgtcaac ttcgcctgaa ggctgccggc
     6001 atcttccagg cgcgccgcat cgaggccatg cttcgtgagc agctgcctgg catcgtccat
     6061 gtgcttgcgc acgacttcat cggacttgcg catcaggccg ggccgcgggg ccgcgccagc
     6121 ggcctcggga gcgcgcatcg ccaggagctg gtcgacttcc gtcgcctggg cgtgcgcggc
     6181 cgtgtcagcg gcgcggacca gggcggcggc cgcgccgagc tcgcggcccg actgcagcgc
     6241 ggccacggtc cgctcgtgcg actcctcgag ggcgtagtag ccgagagcgc cgaccgcgat
     6301 gaccccgacc agcgaccccg cgaacatcat gttgaggagc gaatcagcgg cgagttgaat
     6361 cttcatcttc atcgtcttct cctgttgcga cgccgttgtg ccggcgccgc gtggcttcat
     6421 tccattgcgg actcgaccag cgccatgtcg gcccccgtca gaagccgctc gatatccagc
     6481 atgatcagca tgccgccgtc gaccgtggcc aggccggtga tgtacttggt gtcaaaggtg
     6541 gcggaggcga attcgggcgt ggggcggatc gcctcggcat cgagcgacac cacatcggaa
     6601 accgaatcga cgacgatgcc gaccacgcgc gacgccactt tcaggatgat caccacggtg
     6661 aactcgttgt acggcgtatc cgggagtcgg aacttgacgc gcaggtccag gatggggacg
     6721 atgaccccgc gcaggttgat cacccctttg atgaacggcg gcgcattggc gatggaggtg
     6781 ggcgtttcat aaccgcggat ttcctgcacc ttcaggatct cgatgccgta gctttccgag
     6841 cccaggcgga aagtcaggaa ttcgctcttg cgagcctccg cgttgcgcga atctcccggc
     6901 tgtgaagtgg tgcggtatcc ctgctgctgc attgggtgtc tcctgtggct atgacatgcg
     6961 cacgatatgg gcgacgtcga ggatgagcgc gacggagccg tcccccatca cggtggcgcc
     7021 cgccaggccc ggcaccttgc ggaaattggc ttccaggctc ttgaccacca cctgttgctg
     7081 gccgaccact tcatcgacca ggagggcggc gtaggttcca tccgcctcaa caatgacggc
     7141 gatgccgccg ggctgctccg acgcattgcg gggcggaaag atctcggcaa ggcgcaacac
     7201 cggcaggtac gcatcgcgca cgcggagcgt atctccctgc cccggcagcg catggatctc
     7261 gccgggctcg acgctgagcg actccatcac tgcgcccagc ggcaggacat aggtcgcgtc
     7321 gccggctgcc acgatcatcg cgtcgatgat cgccagggtg aggggaaccc gcacggtgaa
     7381 cgtcgtaccc cgacccgcgg tggaggaaat gtccacggtg ccgcccagcg ccttgatgtt
     7441 gcgccgcacg acgtccatgc cgacgccgcg cccggacaaa tccgtcacct cttccgcggt
     7501 gctcaagccc ggcgcgaaga ccagttccca cacctgcgcg tccggtgcgt ccggcgcgat
     7561 cgcgatgccg cagcgcgcgg cctgcgccag gatgcgctcg cggttcaggc cgcagccgtc
     7621 gtcgctgacc tcgatgacga tggtgcctcc ccgctgcctg gcctgcagcc gcagcgaccc
     7681 cgcggcgggc ttgccggcgg cctcgcggac ctccggcgtc tccaggccgt gatcgatcgc
     7741 gttgcgcacc aggtgcgtca aagggtcgga gaccttctcg atcaggccgc gatcgacctc
     7801 ggtggtctcg ccctcgaact gcagctcgac gcgctttccg aggcggctcg cgagctcgtg
     7861 cgcgaatcgc ggaaagcgcg agaacgcgtt ggagatcggc agcatgcgga tcgcaagcac
     7921 cgcctcctgc aggttcctcg tgtggcgcgc cagatcggcc aggccgccct cgaggcgccc
     7981 gccggcggcg ctgtcgtcca gccggccgca gcgcgccagc atggcttcgg tgatcaccag
     8041 ttcgccgacg agattgacga ggagatcgat cttttcggtg gcgacccgga tgtgcccggt
     8101 gtccgggacc tgcatgacga tctgctcggc cgcctgggcc gacggacgat cggtattcga
     8161 tggttcggcg ggcccgatcg tctcgggacc gggatccggt gcttgcccgg tttcctgcgc
     8221 cagagcctgc ctctttcggc ggaactcttc cgggtcgacg aagaactcgt catgtgcgtg
     8281 cagcgcggcg gccgccccga atgccgcggg agcgagcgtc gcgctctcgc caccgccggt
     8341 cttttcgatc gagatcagct ccggtgccac caacagcgac aagacgctgc gcaagtcggc
     8401 ggccgttccc tcgagctgca catcgaaact gacgcagccg cccggaacgt tctcgatcac
     8461 cgtgtccgtg accgtcccca tctcggatag cccgcccagc atcatgtcca gttccgactt
     8521 ggcaatcggc tcctcgagcg gggcaaggcg aacggcgaaa cgccgaacga ccggtcccga
     8581 cgccgcttgc ctcgcgtcgc tgcgcgggcg ggcgagctgc tcgcgcagca tggcctccac
     8641 ggcgctgccg tccggcggct ggtcgagcac gccgcgatgg cacagggcct gcgcccgcac
     8701 ggcgtccccc gccttgagca tggcctccac atcgcccgcc tcgagcggcc gctcctcctt
     8761 tcgcagcagg tccagcaggc tttcctgcag gtgcgtgagg gccgccatgt cgctgcatcc
     8821 cagcatcgcg gcgctgccct tgatggaatg gacggcgcgg aagatcgcgt tcaggtcgtc
     8881 aagacagggt tgctcgggcg cgaggcgcag caggatagat tcgacattgg caaggtgatc
     8941 ctgcgtctcc tcgaaaaaga tcgcgcggta cgcgttgggg tctccgaggg cgcctgtcaa
     9001 gttgtacatc gaacctccgg ctcagatgaa cttcgccacg atatcgagca tccgctgcgg
     9061 gtcgaagggc ttggccatcc atccagtggc ccccgcctcc cgcccttgct gcttcaggac
     9121 gggatcgact tcggtcgaaa gcacgatcag ggcaaccgcg tcgtagttcg aaagctggcg
     9181 cagggctcgc accagcgtca caccgtccat tcgaggcatg ttgtggtcgg tgaccaccag
     9241 gtcgaattgc cgcgcccgcg ccagttccag cgcctcggcg ccgtcgcccg cctcggccac
     9301 gtcatagcct gccccggcca ggacggcgcc gaccatcttg cgcatcgagc gcgtgtcgtc
     9361 caccaccaga attgccttgt tcatgtcatt tgcgcgaagc gcgctcccgg tggtgatggt
     9421 ggtgcttgcg actgctgggc aggaacttgg cgatcacttc gagcagccgg tcctcgtcca
     9481 gcggcttggt caggtactcg tcgcagccgg cgagcgaacc ccgcaatttg tcgaagggcg
     9541 agctgcggct ggtcagcatg accacagccg tcttcttgat cgcctgcttg ttcgacttga
     9601 tcaacttgca gacctggtag ccgtcgatgc cgggcagcac cacgtccagg aacacgcagg
     9661 tgtattcctg ggtggcgctc aggccgatcg cctcctcacc ggtttcggcg atatccacct
     9721 cgaaaccgaa gggcgccagc ttggcctgca tgaacatgcg caccgtggcg ttgtcgtcga
     9781 cgaccagcac cgtgtcgccc agcactcgct tggtcgcctc ggcggcaggc atctcccgca
     9841 gtgagttgga gcgtgcgagc gaagcgccgg acaggctgcg gcgcaggccc tgcggtgtct
     9901 gcggcgccgc tgtcgcggtc gacacgggcg gctcctggcc gccgccggcg agcgtatcgt
     9961 ccagcgcctg cagcagcctc gcccactgca gcggccgcgg aatcaccagg caattcgtcc
    10021 cctgcggccc cgcgccgacc agtaccgccg gcaatcgcgc ccgccggtgc agcgcgcgga
    10081 attcgccgaa tgcagccgga ttgtcggcgt ccaccaggta cacgtccggc ttgcgtccgg
    10141 cttcaccggg ctcgtactgc gcaaagccgg ggtcgcgccg tgcggccagc gagaagatgc
    10201 tcgaaagcat cgatcgctcc acgtcattga agccgatgac gtcgacgaga tattggtcgt
    10261 tctggttcaa cttgggttcc tgctctcgtg cgatgaacct cggccgtgcg gctgccaaag
    10321 aacgcgccct gcgcgctcga gtagcgcaag gacggcccgg ccgtcggccg gacgttgcgc
    10381 agggtccttc gccagcatgg cgtccaacag aggctgccac gcggcctggt cccgcgccag
    10441 ccgtggtatc gctgccagca ggtgctggcc gagcaattcg gccaaggtcc ggccgggata
    10501 gagcggcttg ccggtcaaca tctcgtacag gcagacaccc aggctgtaga cgtccgccgt
    10561 gggttgcgcc ggtgcccccg cgctttgttc cggggccgca taacgcggcg ttcccaccac
    10621 ggtccccggc ggcaaggcac cggcgttgcc gcgccggcag gcgcagccga agtctcccaa
    10681 tgccaggctg ccgtccgccc gcagcagaag attggctggc ttcacgtccc ggtgcaccca
    10741 gccattcgtg tgcagccatc ccagggcgct ggccgcctcg cgcaagcgcg tgaccatgcg
    10801 agccgcgctg accggggcgc tatagggcgc catccggccg cccgcggcgt attccatcgc
    10861 gagataagcc tcgccgtccg actcgccatg gtcgaacacc cggatcacat gggagtgagc
    10921 gagcgccgac agcaccccga actccccacc gaagtcttgt ttcgcgtcca tgccttcgag
    10981 ccgcgtctcc cggccgacct tcagggcaac gatttgggcg ccctgaggct gacgcgccag
    11041 gtaaacgatg gaatggcggc cacgacccag cacggcttcg agccgatatc ccgccaggga
    11101 gtgcgcaagc ccgagtgccc ccgcatcctg tcgtcgtggg gacgcggcgt gatccgccgg
    11161 cccggagcgg ctccggggtc gtatgaggac ggcggcaagc gcgctcattg ggccagctcc
    11221 tccaatgcgt ccagcagcgc agtggccgac gcatagcgct gcgccgggtc cttggccatc
    11281 attttttcca gcacgggctg aaaccgctga agtgccgcgg gcagggccgg cacgggggcg
    11341 ttgacgtgca ggtccagcag tgcccgcgcc gtttcggcat ggtatggttt catcccggtg
    11401 aacatctcgt aggcgacgat gccgaggctg taaaggtcgc agcgcgcgtc caccggcttg
    11461 ccgagcacct gctccggact caggtagtac ggggtgccga tgatgtcgcc cgcgccggtg
    11521 tcggtgattt ccatcgaaac ctgcttggcc acgccgaaat ccgcgatcgc cagcattcca
    11581 tcctggcgga tcatcacgtt gtcgggcttc aggtcgcggt gcacgatgcc gacttgatgg
    11641 atggcttcga gcccggcggc gatttgcttc aggtagtaaa gggcgatgcc gggatccagg
    11701 gcctgtcgca tccggctgcg caagtcaccc agcggaaaat actccatggc gatgtacccg
    11761 ctgccccccg agaagtcctg acggaaaatg cgcgccacgt tcgggtggtc ggtctgggcc
    11821 agcagggcga attcctggat gaagcgctgc aggtccatct ccccttcgcg atccggccgc
    11881 agcaccttca acacctgggt cgggcctccg accattggct gagccaggta gacggtggca
    11941 ttgccgccct cgccgatctt gcgcagcagc cggtagcctt cgatcagcac gtgccccgga
    12001 ccctgcaccg cttgcgggtt ggctgccgcg gcggcattcg cggcggccct ggcaagctgc
    12061 tggttacgct cgagcgagtt gcgcagcgcc tcgtaatggc ttggcggcgt gaccgagcct
    12121 ttgcgcggga cgaggcccga gatctcgacc acctcgagga atgtgtcgtc gggcaggccg
    12181 aacgtggcat ggcggcccga ctccaccctg gtgcccgccg cctgccgcgt ggccaatgaa
    12241 gtgaccacgc cccaatgcaa gtccgccgca acgatgggaa gcaggcgggc gatctcgacg
    12301 gcttcgcccg tggcatgcac catgccgtgg ctattcgtcg cgcgcggtac cacctcgccg
    12361 gcgcccaggt gcacgccaac cgcgatggac agcggcggca gcgccgacga ctggggcctg
    12421 gccgcaatgc gcgtggcgag ctgcacgaac tcgtgcactg tcaggatcgc ggcatgcaga
    12481 gcgcgcttgg catgaaccgg gaggcgatct tccggcttgt gggaaaagac gcagaggatg
    12541 gagtcgggcc gccgctgggc gatctccccg cccagcttga gtcccgccga cgagagggct
    12601 cgccggacct cgttcacgaa cgaggtcagt tcggaagccg acatcgtctc gcatgtcacc
    12661 gcgaaattgc gcagccgcgc atacatgaat gtggcgagcg tgccctcagc ttccgcgctg
    12721 gcctcgggtt cggggcgggc ggactcatag ggcgaaaccg acgaggcgcc aaacaacgac
    12781 tcgagcgggg ccacgtcggt cggcgcgaag tgcgcgtttg cgtcctgcga tgtttccccg
    12841 cccatctttg catccacgag ggtcaagcag atgcgttcga gcccgcacgg caatggggag
    12901 gtgcgccgcg ggacggctcg acccctcgca tgcgatgcgc cgtgcgaggg caagcgcgtt
    12961 ttcgtgtgct gaatgccatg ccttttattg ctgagttcga ccctttgtag ccgagtccgg
    13021 cgctcatgtc acgccttgtt actcactcga ccatgcaaac gtagcccatc gcagacagat
    13081 aggtataaat aggtacaaac tctgctggcg atgccattgg aggtcagggg tgccacgtgg
    13141 gcaatccggc agcacaggcc gccgcggcgg ccatcgcgcg aaaacgagac ggacagaacc
    13201 ctacccgcgc ggatgatgcg cggcgtgcaa ttgtttcagc cgctcccgcg ccacgtgggt
    13261 gtagatcgtg gtggtcgata tgtcggcatg ccccagcagc atctgcacgg cgcgcaggtc
    13321 ggcgccgtgg ttgagcaggt gcgtggcgaa ggcatgacgc aaggtgtgcg gcgacagcgg
    13381 cgccgtgatg gcggcgctgc gcgcgtattt cctgacgatc atccagaaca tcacgcgggt
    13441 catgccctcg ccccggccgg tgacgaacag gtccccgctt tgctgcccgc ccaggatcac
    13501 aggccgcgcc tcggtcagat aacgggtgat ccagtcgcgc gccaccaggc cgaagggcac
    13561 cagccgctcc ttgctgcctt tgccgagtac tcgcagcacg ccctcgccca tgctgacgtt
    13621 gaaggtcttg agcgagacca gctcgctcac gcgcaggccg ctcgcgtaca tcagctccag
    13681 catggtgcgg tcgcgcaggc ccagcggctt gtccacctcc ggggcgtcca gcagggcctc
    13741 gacctgcgac tcggtcaggc tcttgggaac tcgcagcggc tgcctggcgg attgcaggcg
    13801 cagcgtgggg tcggccgcga tcaggcgttc ccgcagggcc cagcggaagt agcgcttgaa
    13861 cacggtgagc cggcgattgg cggtggtcgc cttgctttgg gcatggcgcg ccgcgaagta
    13921 gccgttgagg tcgccctcac cggtgctgtc gatggtgcgg cccagttcgg caagccagcc
    13981 ggcgtagagc gacaagtcgc gccggtaggc cgccagcgtg ttcctggaaa gcccatcctc
    14041 cagccacagc gcgtcgatga atgcgtcgat tgaagcattg ctcatggcgc gaaagatagc
    14101 aaaaaggaga cagccgcccg cgcacgtgca ggttcagccg cgccaaggac aagacgccag
    14161 ggcgcgctgc tcttgtcaag ccaccgtgga aggcggcctg ttcacctcat tccaacttta
    14221 attttgcgct ctccaccacg ctcttgtaca ccgcgtactc cgccctgatc tgctcggcga
    14281 actgctgcgg cgtattcccg acgatcagcg aaccggtttc ctcgatgcgc ttgcgcaccg
    14341 ccgggtcctc cagcaccttc ctggtcgccg cgtggatctt ggcgaccact tccttgggca
    14401 tgcccttggg cccaaggatg ccgtaataag ccatgcggtt caccggctcc aggcccactt
    14461 ccttgaaggt cgggacgttg ggcaggctcg ccaggcgctg cggcgctgcc accaccatcg
    14521 ggaccaggcg gccgtccttg atgaagggct gggccgacgg caggttgtcg aaaatgatcg
    14581 gtacctggcc ggccacggtg tcgttcaggg ccgggccggc gccgcggtag ggaatgtggg
    14641 tgatgaaggt tcccgacagg ctcttgtaca actccatctg caggtggccg atgccgcccg
    14701 tgcccgagga cgcgaacgaa tagcggcccg gcgaacgctt gagctcggcc acgaaggcca
    14761 ggtaggtctt ggcggggaag ctgggatgaa ccgcgatcac gttcggcgtg gccgcgatgt
    14821 tgatgatggg cgtgaagtcc gtcgtcgggt tgtagggcgt cctgggattg atggccgggt
    14881 tcgccgccgt ggtcgacacc gtcgccatcc ccagcaagta gccgtccggc gccgaccgca
    14941 ccgtctcgag cgcgccgatc acgccgccgc cgccggcccg gttttccacg atcaccggct
    15001 gccccagcgc cttgcccagc ggatcggcaa tcacccgggc gatgatgtcg gtcgtgccgc
    15061 ccggcgcgaa cggaacctgc agtttgatgg gcttgttcgg gtagccctgc gcaagcgcgg
    15121 ggccggcggc cagcgccaat gcggcagcgg gcaggacaaa ccaaagacgg cgttgtttca
    15181 ttgggagact ctccttgttg gggtcgaaaa acacgaggct gcggcatcct atcgtcggaa
    15241 tgcgcggcgc gatgttgtgg attccacata ggcgcttacc ctagagagtg ccggccttgc
    15301 tgagcttgcc gttcgcgcta gggctgctgc gttgactctt gcgctgacgc ccacgccacg
    15361 tgttcgcgga ccagggcgct cgggtgatcc gcgcgcgttt gcaatgccgc ccgcagcagc
    15421 ggatcctccc gcacccgcag cgcattcccg atcgccacgg cgatgttgcg cagccagcgt
    15481 tcatggccga tgcgccggat cggtccgccc tgcgtatggc gaagaaactc ctcctcgctc
    15541 cagccgaaca gctccacgag ctggcgggcc gtgaggccac cgcgctcgtc gaagtcgggc
    15601 agggcgctgc gccgcgcgaa cttgttccag gggcagatta gctggcaatc gtcgcagccg
    15661 tagatccggt tgccgatcaa gggccgcaac tcctcgggga tgggcccggc atgctcgatg
    15721 gtcaggtagg agatgcagcg ccgcgcatcg agccggtacg gcgccacgat cgcctgtgtg
    15781 gggcaaacct cgatgcacgc gctgcaggtg ccgcagtgac cgcgggccgg atcgctgacc
    15841 ggcagcttca tgtccacgta gatttcaccc aggaagaaca tggaaccggc ttcgcggttg
    15901 agcaccaggg tgtgcttgcc gcgccagccc tggccgctgc gtgccgccag ctcggcttcc
    15961 agcaccggcg cggaatcggt gaagacccgg tggccgaacg ggccgatccg cgaggcgatg
    16021 cggtcgctga gtttgcgcag ccgcgaacgc agcaccttgt ggtagtcccg gccccgcgca
    16081 tacaccgaca ccgcaccttc gcccggtcgc cgcagccggt cgaactcgac cgcttgccag
    16141 ccttcgggcg ttgcggccgg caggtaatcc atccgcgccg tgatcacgct ggccgtgccg
    16201 ggcacgagtt cggccggcct ggcccttttc aggccatggg tttgcatgta agccatttcg
    16261 ccgtgaaagc cgttggacag ccaggccagc aaaccaggtt cggcggatga aagatccaca
    16321 ccggccacgc caatttggga aaatcccagc tcaagggccc attcccggat ttgccccacc
    16381 agtcgaggac cgtcgatctg accactgttg aacatcaccc gcccattgta ggaatcgcgc
    16441 tcgcgcccgg tgcggtgggg gctcccctgg acgccgcggt ggtcgtctgg cgcaacgagg
    16501 acgatacccg cgccttcgcg gcgaagttgg cggcccaccc ggcgatcggc ggggccttca
    16561 tcgagctgca cggcgacctg ggcgccggca agaccacgct ggcgcggcac ctgctgcgcg
    16621 cgctgggagt ccaggggcgc gtgaaaagcc ccacctacac cgtgctcgaa ccgcacgaag
    16681 cgcgcggcct cgcgatctgg cacttcgact ttttccgctt caacgatccg cgcgaatggg
    16741 aagatgccgg ctttcgcgac atcttcgccg gaccgggcct gaaactggcg gagtggccgg
    16801 agaaagccgc cgggctgccg ccccgggccg acctggtggt gcgcatcgaa gcgcaggccg
    16861 acgaatcccg caacgtcacg ctcctggcgc agacgccgct gggccgggag ctgctggaat
    16921 gaaaaggcgc gagctgctgc gtcgcggcac gctggtcctg ctgctgggca cccggcagat
    16981 cgcccgcggc gcgaccatcg tcgccgtgcg cgtgtggccg gcgccggatt attcgcgcgt
    17041 cacgatcgag tcggaccggc agctcagttc cacccagatc gtcatggcca acccgccgcg
    17101 cctggcggtt gacatcatgg gcatcgacct catcccggag ctgcgcgagc tggtcgccaa
    17161 ggtcaaggcc gacgatccct tcatcgaggg cattcgcgta ggccagcagt cgccgggcgt
    17221 tgtacggctg gtggtcgacc tgaaacaggc ctcggtgccg caggtgttca cgctgacccc
    17281 ggtagccgct taccagcacc gcctcgtgtt cgatttctat ccgctcaaga cagtggaccc
    17341 gctggaagcg ctgatcgcgg agcggctcaa ggaccagccg gtggcgccgc cgacgcctgc
    17401 cagcgacccg ctgggggaat tgatcgccca gcggctggaa ggagacaggc gctcgggcgt
    17461 gattgccagc gccgaagccg cgccccggcc cgcgccccag aaaaccgacc gcctcatcat
    17521 catcgcgctg gaccccggcc acggcggcga agatccgggc gcggtggggc gagcaggtac
    17581 ccgcgaaaag gacgtcgtgc tgcagatcgc gcatcgcctg cgcgagcgca tcaatgccag
    17641 cgtgatcaac ggcaacccga tgcgcgcctt cctgacacgc gacgccgact tcttcgtgcc
    17701 gctgcacgtg cgcgtgcaga aggcgcggcg cgtgcaggcc gacctgttcg tgagcatcca
    17761 cgcggatgcc ttcctcacgc ccaccgcccg cggcgccagc gtgttcgcgc tcagccgggg
    17821 cggtgcgtcg agcaccgccg cgcgctggat cgccgacaga gaaaacaggg ccgacctgat
    17881 cggcggcgtg aacgtcaagg cgcgggacgc gcatgtggcg cacgcactgc tggacatgag
    17941 cacgaccgcg cagatcaacg acagcctcaa gctcgggagc gcattgctgg gcgagatcgg
    18001 caacgtgggc aggctgcaca agggccaggt ggaacaggcc agcttcgccg tgctcaaggc
    18061 gccggacatc cccagcgtgc tggtggagac cgcgttcatc agcaatccgg aggaagaggc
    18121 gcggctgcgc agcgatcagt accaggaaca gctggccgac gcactgatgc gcggcatcca
    18181 ccgctacttc gcgaaaaacc cgccgctggc ccgcagccgg gcgctgtgaa cgccagcgcc
    18241 tgagctgtgc cggacaaagc cgctcggcgg ttgcggcaag agccgacggt tcgcgcctcc
    18301 cttctcctac acccccgtgc agcgccgccg cctagagttg tgacacaggc aatgcaagca
    18361 gcgtgtttgc gctgc
//
LOCUS       DASWIY010000026         3796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_390027, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000026 DASWIY010000000
VERSION     DASWIY010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390027"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VGA59_00950"
     CDS             <1..668
                     /locus_tag="VGA59_00950"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HEX9718273.1"
                     /translation="GLRLYLIPHLALFDPAWLPNLQAFVDGGGWLMVGARTASKDLND
                     NVVPETLPGVLRPLVGASVEEYGRQNRPERRPLRMRVGDTELATELWYELLQPDAGTE
                     VVATWTTRHLAGGAAVTLRRHGRGGVMYVGTYLTSVVTEALLPMLGRLGAMPTPLSSW
                     PKIEVTERIGTGRTFRVVINHHDEPAQLPWPADAGEELLGHEVMGGELHLAPHDVALF
                     RMQ"
     gene            680..2248
                     /locus_tag="VGA59_00955"
     CDS             680..2248
                     /locus_tag="VGA59_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HEX9718274.1"
                     /translation="MNTSAWREIEIALTSARVYANPYTDVAVWADFTAPTGETWRRPA
                     FWDGGRSWKIRFAPPGVKGRWSWRSYSSSADAGLAGQQGDVVCEVEPGPASEHRFLRH
                     GFWRMSPRGRNLIHADGTPALLAADTAWALPWRATEEQCRIYAADRQVKGFNTALLMS
                     VQPDMRARGPRDRTADEGFDVGFEDLPDGRLNQLNPAYFQYLDRLLSILVEHEIVPVL
                     QPVFMGFGWKGLEVAGPVVPPAEYARYCRYLVARYGARPAIYLVGADGTGYEPQVAAG
                     GEEVECWDCYRQPTGIHYRPHADNRAHQEAVWLDFQWCQTGHFGEHVPERVADMWRNT
                     PVKAVANGEPTYEQPGAQAKGIGWWQGHEAWCNLCAGGTMGVAYGAASLWQWRLHPDE
                     PGHASFFLAEASGWREALEAEGSRYVGLVPQILEGLPITDMEPNWQVTLGRRGLLVPG
                     KLYIGYAAMGGLLHIVGDGVPRNFRVIDPRNGRIVREGTRESARTPIVDSGNEPRIYL
                     CYDEDWPQNGPRQS"
     gene            2523..3623
                     /locus_tag="VGA59_00960"
     CDS             2523..3623
                     /locus_tag="VGA59_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9718275.1"
                     /translation="MDFAFLCLACVLWRFKNQNLECRPIWQGQSGFTSGARWATMQSA
                     ASPRAMENRIERIEHQENLLLRVFVHSVRSCASHLHRDCEFLLVLRGQVLVQCGGLES
                     ILKADDVFFVPGGEVHLTHEATGSNLLVALQVDMDFAMQLDPDFPQRRFGLNHLARTR
                     PADPRVRTVRQIMAEIMWEMRLKRPAYRLQVESLVLRLLTLLVRQIESTLIQTVPEVR
                     AADEDALGQRLVRITAYLEAHSSEELSSADLAASEGVSVSYLARLFKERLGCTFSDYV
                     NMQRARNSLALLARKEESILDIALECGFPSVKSYNAVFKRIYGVPPSQWRRQQAGADI
                     PGVGSSAYHRHDPGLAYHLLKKYLPAGTSVVA"
BASE COUNT          659 a         1202 c         1264 g          671 t
ORIGIN      
        1 gcggcttgag gctgtatctg atccctcatc ttgcgctttt tgatccggcc tggctgccca
       61 acctgcaggc ctttgtcgac ggcggcgggt ggctgatggt aggcgcccgt acggcctcca
      121 aggacctcaa cgacaacgtc gtgcccgaaa ccctgccagg ggtcttgcgc ccgctggtgg
      181 gggcaagcgt ggaggaatac ggccggcaga accgccccga gcgccggccg ctgcggatgc
      241 gcgtgggcga caccgagctg gcgaccgagc tctggtatga gctgctgcag ccggacgccg
      301 gcaccgaggt cgtggcgacc tggacgacac ggcatttggc cggcggtgcc gcggtcacct
      361 tgcggcgcca tggtcggggt ggggtgatgt atgtcggcac gtatctcact tcggtcgtca
      421 cggaagccct gcttcccatg ctcggtagac tgggcgcgat gccgacgcca ctttcctcgt
      481 ggccaaagat cgaagtgacc gaacgcatag ggacagggcg gacgttccga gttgtcatca
      541 accaccacga cgagcctgcg cagctcccgt ggccggccga cgcgggggag gagctgcttg
      601 gacatgaggt tatgggtggc gaactgcatc ttgcgcccca tgatgtcgca cttttccgta
      661 tgcaatgacc cggctcacta tgaatacaag cgcctggcgg gaaatcgaaa tcgcgctcac
      721 ctcggcgcgc gtctacgcga acccctacac cgacgtcgcc gtgtgggccg atttcaccgc
      781 gcccacgggc gaaacgtggc gtcgaccggc cttctgggat ggcggacgca gctggaagat
      841 tcgcttcgct ccgccaggcg tgaagggccg ttggtcctgg cgaagctaca gctcgtccgc
      901 tgacgcgggt ctcgcgggcc agcagggcga cgttgtgtgc gaggttgaac ccgggccggc
      961 gagcgagcat cggttcctcc ggcacggctt ctggcgcatg tcaccccggg gacgaaacct
     1021 gatccacgcc gacgggacgc cggcactgtt ggcggccgac acggcttggg ccctcccctg
     1081 gcgcgcgacc gaggagcaat gccgcatcta cgcagccgac cggcaggtaa aaggtttcaa
     1141 caccgccttg cttatgtcgg tgcaacccga tatgcgcgct cgtgggccgc gcgaccgcac
     1201 cgccgacgag ggattcgacg tggggtttga agacctcccc gatgggcgcc tcaaccagct
     1261 caacccggcc tactttcagt acctggaccg gctcctctcc atcctcgttg agcacgagat
     1321 cgtcccggtc ctccagcccg tattcatggg gttcggctgg aaggggctgg aggtggccgg
     1381 gccggttgtg ccgcccgcgg agtatgcgcg ttactgtcgg tatctggtcg cgcgctacgg
     1441 agcgcgtccg gccatctatc tggtgggcgc cgatggcacg ggctacgagc cgcaggtcgc
     1501 ggccggcggc gaggaagtgg agtgctggga ctgctatcga cagccgaccg gcattcacta
     1561 ccggccgcac gcggacaatc gcgcgcatca ggaagccgtg tggctcgatt tccagtggtg
     1621 ccagacgggg cattttggcg agcacgtgcc cgagcgcgtg gctgacatgt ggcgcaacac
     1681 ccctgtcaag gcggtggcca acggcgagcc cacctacgaa cagcctgggg cacaagcgaa
     1741 aggtatcggc tggtggcagg gacacgaggc ctggtgcaat ctttgtgccg ggggaacaat
     1801 gggcgtggcc tacggcgcgg ccagcttgtg gcaatggcga ttgcaccccg acgagcccgg
     1861 gcacgcgtcc ttcttcctgg ctgaagcctc cggctggcgg gaagcccttg aggcggaggg
     1921 atcacgctac gtgggcctgg tcccgcaaat tctcgagggc ctgccgatta cggacatgga
     1981 gccgaactgg caggtcaccc tcggccgccg ggggctgttg gttcccggga agttgtacat
     2041 cggctatgcg gcaatgggtg gcctgctcca tatcgttggc gacggcgtcc cgcgaaactt
     2101 ccgcgtcatc gatccgcgca acgggaggat cgtgcgcgag gggacccgcg aatccgcccg
     2161 gacgcccatc gtcgattccg gcaacgagcc gcgcatctat ctctgctacg acgaggactg
     2221 gccgcagaat gggccgcgac aatcgtgaga tgagcgggcc gatttgcagg acggggcgaa
     2281 ccgcggttat cagcaataag atgccagcaa ggggacgtct aaaatgcctc gcgccaaggt
     2341 ggatgctggc ccccggacgg cgtattctgc actcgacgaa gagcgcagtc ggggcccagc
     2401 ctttgccgga ggcttcggcg ggcaggctgc ccgcggccac ctgttgcggc acgcagaatg
     2461 gcgggtgcca cggttctgga tgcccgatga gacgacctca tgctagtcca cacggagctt
     2521 gggtggattt tgccttcctt tgcctcgctt gcgtcctttg gcggtttaaa aaccagaacc
     2581 tggaatgccg gccgatttgg caagggcaat ccggctttac gtcgggcgca cgatgggcca
     2641 caatgcaaag tgcggcgtcc ccccgcgcca tggaaaaccg catcgaacgg atcgagcacc
     2701 aggaaaacct gctcttgcgg gtttttgtgc acagcgtgcg cagttgtgcc agccacctgc
     2761 accgcgattg cgaattcctg ctcgtcctgc gcggacaggt gttggtccag tgcggtggcc
     2821 tcgagtccat cctcaaggcg gacgacgtct tctttgtgcc cggaggcgag gtgcacctga
     2881 cgcatgaggc aacgggttcc aacctgctcg tcgcgctcca ggtggacatg gacttcgcga
     2941 tgcagctcga ccccgatttt ccccagcgtc gattcggatt gaaccacctc gcgcgcacgc
     3001 gccctgccga tccgcgggtg cgtacggtgc gccagatcat ggccgaaatc atgtgggaaa
     3061 tgcggctcaa acggccggcc tatcggctgc aagtcgagtc gctcgtcctg cgtctcctca
     3121 ccctcctggt gcgccagatc gaatcgacgc tcatccagac ggttccagag gtgcgtgcgg
     3181 cggatgagga tgcgctgggg cagcggcttg tccgaatcac tgcctatctc gaagcccaca
     3241 gcagcgagga gctttcgagt gccgacctgg ccgccagcga aggtgtgtcc gtcagttatc
     3301 tggcgcggct cttcaaggaa aggctcggat gcacgttcag cgactacgtc aacatgcagc
     3361 gggcgaggaa ctcgctcgcg ctgctggccc gcaaggagga gtcgatcctg gacatcgcac
     3421 tggaatgcgg atttcccagt gtgaaaagct acaacgccgt cttcaagcgc atctacggcg
     3481 tgccgccctc ccagtggcgc aggcaacagg cgggcgccga cattccgggc gtcggttcca
     3541 gcgcctacca ccggcacgat cccggtctcg cctaccatct gcttaagaag tacctgccgg
     3601 cggggacgag tgtggtcgcc tgagcacagg caattcggtc ccgtgacgga aagagcagac
     3661 atcttcgacg acgcggggtc gtcctgcccg ggtttacggc cacctcttcc catctgaccc
     3721 gtgcctgtga caccacgcaa agtgccgggg agagaacgcc cgatgtccaa aggaaaaact
     3781 gcccacctca gtcacg
//
LOCUS       DASWIY010000027        16777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3346096, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000027 DASWIY010000000
VERSION     DASWIY010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 16777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16777
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3346096"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..1092
                     /locus_tag="VGA59_00965"
     CDS             4..1092
                     /locus_tag="VGA59_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX9718276.1"
                     /translation="MILRDITERLQAEERNIRLAARLAGLLDSAMDAIITVDDRQRVV
                     LYNRAAEKIFGWTSEEAIGGPLEKLMPERFRGSHGAHVDRFDRTGTTSRRMGDGTVLY
                     GRRANGEEFPMEASISQLDTAEGKLFTVILRDISERIRTQEELSSFAAEAHTIREGEK
                     SRIARELHDELAQALTALKMDTIWLRDNAPAQPDKAVAKLAEMVSMLDTTVAATRRIA
                     ADLRPLLLDDLGLAPAIEWLAHTFTQRTGVPCAVSVDEELELPEPYATAVFRIVQESL
                     ANVGKHAQATEAGVVIARTGQAVMLEVRDNGQGFATAAPRKPHSLGLMGLRERAQLLK
                     GTVSIDSRPGKGTRVQVRIPVQEIGAAT"
     gene            1089..1733
                     /locus_tag="VGA59_00970"
     CDS             1089..1733
                     /locus_tag="VGA59_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9718277.1"
                     /translation="MIRIVIADDHAIVREGLKRIVSDAGDMLVAGEAADGTEVMKRVR
                     ELEFDILVLDLSMPGRSGMELIRMVRAEKPKLRILVLSMHQELQYAVRAIKSGASGYL
                     TKESAPAQLEQAIRKIAGGGAFITPEVAEQLALGAMPGSETHPHETLSDREFEVFRML
                     AAGVSVTEIAARLKLSVKTVSTHKANLMQKMGLQNASELIRYAIRHGLADSLEP"
     gene            complement(1745..2056)
                     /locus_tag="VGA59_00975"
     CDS             complement(1745..2056)
                     /locus_tag="VGA59_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718278.1"
                     /translation="MMVARWSIDAKFGYKQNVVDMMQRWLREVGPKAGIKPEQTRLLT
                     GSIGALEATVQSEHVIENLAELNQVWDRLAALPEHQKWGKELEPHVVSGTSRWEIFRL
                     L"
     gene            2261..3097
                     /locus_tag="VGA59_00980"
     CDS             2261..3097
                     /locus_tag="VGA59_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9718279.1"
                     /translation="MKESLTQLLVHLDASPGASQRLEVARAIAQSHGAAVTALYAVTP
                     ALLVVPFAPEAGPSVATTLREIDDERRAAARAALERSLATPGVHAAWAEVRDYPIVPA
                     FSRQALYADMLVLGQHDPAAPSPGVPSDFAQTVMAASGKPAIVLPYAGVPSAVGETAV
                     IAWKPTREAARAVSAAVPILQRARRVHVLSWMEEQEGIDGARLDLDGYLKLRGIEPVW
                     HLEATEPDAVGELLLTRAFDLDADLLVMGCYGHSRAREWVLGGASRTVLGSMTLPVLM
                     AH"
     gene            3290..3688
                     /locus_tag="VGA59_00985"
     CDS             3290..3688
                     /locus_tag="VGA59_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX9718280.1"
                     /translation="MPTRPKPDWILIANTTRARVLQQEPGNAMVVLESFVHPAGRGLR
                     TEPLIDARQKECTRFAHELAQFLEQEARQGHFGCLTLFASASFLEDLRASLGKVTTRL
                     LSGTHELDLTNVGVAELERRVSHELALSAH"
     gene            complement(3982..4272)
                     /locus_tag="VGA59_00990"
     CDS             complement(3982..4272)
                     /locus_tag="VGA59_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HEX9718281.1"
                     /translation="MPTLTNAAGRADILTRLKRAEGQLRGIQRMVEEGSPCLDVAQQF
                     SAVRKALDSTYVRMTMCMLEQELEAHLQDHGKPDTDLGPLMADVQKLLTRMA"
     gene            complement(4272..4718)
                     /locus_tag="VGA59_00995"
     CDS             complement(4272..4718)
                     /locus_tag="VGA59_00995"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9718282.1"
                     /translation="MAEDTIRVELTQQRDYSFTVRFGGEVPDLLVDEPAPLGGGKGPS
                     PKHLLGAAVGSCLSDSLLFALRKFKQKPEPITCSVETIVGRNAQGRLRVQSMKAVLTL
                     GVAGSSLEHLDRALDQFEAFCTVTQSVGEGIAIAVEVWDATGARLK"
     gene            complement(4727..4951)
                     /locus_tag="VGA59_01000"
     CDS             complement(4727..4951)
                     /locus_tag="VGA59_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX9718283.1"
                     /translation="MTVERYIHIFAGVFILVSLALGIDGSPLFVSHWALAFTAFVGAN
                     LFQFGFTKFCPLGFMLKKLGVPETTASCGN"
     gene            complement(4981..8322)
                     /locus_tag="VGA59_01005"
     CDS             complement(4981..8322)
                     /locus_tag="VGA59_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9718284.1"
                     /translation="MTQDQTSASSQPIQRLGVSGRIAAAFQDNAITPLLAIVALLLGL
                     FAVLVTPREEEPQINVTMANVLIAFPGASSADVQNMVARPAEQVLSQIAGIEHTYSVA
                     RPGLAVLTVQFKVGVPRTEALVRLYDVLNANQDWLPRDLGALPPIVKPKGIDDVPVLA
                     VTLWSRDVLAANDLERVARTLEAELKSVAGTREVQTIGGPGRAVHVWLDPVRMRDRGV
                     DVLGLKATLAAANLGMPSGAVLDASDATARMLTVETGEFLRSEEDIASLVVGVSQGKP
                     LYLREVARIETGARLPQRYVWMTPGAAFAAGDKDAAAAAQTGQVFPAVTITVTKKPGE
                     NAVDVASAVRQRVESLRNTVIPANIETTVTRDYGETAAEKANKLMQKLGFATASVIVL
                     VGLALGRREAAIVGAAVILTLTATLFASWAWGFTLNRVSLFALIFSIGILVDDAIVVV
                     ENIHRHQQLHPGRPLREIVPGAVDEVGGPTILATLTVIAALLPMAFVSGLMGPYMSPI
                     PINSSLGMAISLAIAFTVTPWLALKLMKPHHGHSHTPGHAGGLTGRVQTLFTRLLTPL
                     LSSARKRWLLLAGILAALALSVGLVVVQWVVLKMLPFDNKSEFQVVVEMPAGTPLENT
                     AAALHEMGGWLARQPEVRDLQGYAGTASPITFNGLVRQYYLRAEAEQGDLQVNLAGKH
                     QRTEQSHAIAQRLRPALEEIGARHQARVKIVEVPPGPPVMSPLVAEVYGPDEAGRQRL
                     TQRIAAAFSGTPDIVGVDSSLKEDAQQVHLTVRRQRAESLGIPVSAVAQTVHSALSGV
                     DAAYLHDGQSKYPVPVRLQLPLADQVGLDALLALPMRSASGRMVPLSELVQLDRGTID
                     KPLFTKDLKHVSYVFADVGLRTGSSARQGTPDSPLYGLFGIRAKLDAAALAGTGELGE
                     YWIRQPADPYRAYAIKWDGEWQITYETFRDMGAAYAVGLVLIYLLVVAQFRSYLTPLV
                     IMAPIPLTIIGVMPGHALLGAQFTATSMIGMIALAGIIVRNSILLVDFIELQVARGVP
                     FRGAVMNSAAVRAQPIALTGLAAMIGGFFILDDPIFNGLAVSLIFGILVSTLLTLVVI
                     PVLYYALYRQRFEAEEPPEGNVSAQ"
     gene            complement(8330..9424)
                     /locus_tag="VGA59_01010"
     CDS             complement(8330..9424)
                     /locus_tag="VGA59_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9718285.1"
                     /translation="MIHSSISLVTAVAGVLRLASLAGLLAAAAAWGGPAIVVPVVKAS
                     AQAVPVGFELDGVVEPVKQSTVAAQTSGRISQLAIKAGDRVRTGQLLAVIDDRESQAG
                     LQRSRAQTAQAEAELRNAQAQVQRTRELQAKGFVSKAALDTAEATFKSAQASRDQATA
                     GQRQSAIAQEFTRVTAPYDGWVLETLAQPGDLAVPGKPLLTLYAPLPLRAVVQVPASR
                     AAAARSAVEVEVQIPNGKGGAQWITPTVRAMLPAADPVSQTLEWRLELPAAAAASVAP
                     GQQVHVRFAASPQRRLVVPASAVLRRGELTAVYVASPDGAGFVLKAVRLGADHGAAGV
                     EILAGLNAADSVALDPVRASLAGARPSLAP"
     gene            complement(9556..10128)
                     /locus_tag="VGA59_01015"
     CDS             complement(9556..10128)
                     /locus_tag="VGA59_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX9718286.1"
                     /translation="MKFTTKIIAGAAAALSLALAGAVYAHPDGGSWGMGPGWGMGPGM
                     GHGWGMGAGMGPGAGHMGYGMRGWMGGADMEAVAAGRAAELKVLLKIAPAQETAWKAY
                     ETALRQQATTMQALRTQMHAQMQNQAPGNTEWTAQRDAMIKQHDASWAANTAALKDLY
                     AVLTPEQRVLADRGPMGIGGPRAGRNFPGR"
     gene            10356..11081
                     /locus_tag="VGA59_01020"
     CDS             10356..11081
                     /locus_tag="VGA59_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9718287.1"
                     /translation="MDRSDHILIVDDDAEIRSLLSRYLQKNGLRATAVADGRAMWQAL
                     DRGSFDLIVLDLMLPGDDGLTLCRTLRARSDMPVIMLTARGEETDRIVGLEMGADDYL
                     AKPFSARELLARIKVILRRARSLPPNLQPEEEREIHFGDWSLDTVHRHLVSPAGVVTP
                     IGGAEYRLLRIFLEHPNRVLSRDQLVEMTQGKEADALDRSIDVQVSRLRRRLGDGSRD
                     PGMIKTVRGEGYVLSVPVNKASS"
     gene            11081..12562
                     /locus_tag="VGA59_01025"
     CDS             11081..12562
                     /locus_tag="VGA59_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9718288.1"
                     /translation="MHAWTRWLPRSLFGRLMLVLASGLLLAQLLSAAINLAERDRLLL
                     RARGTQQAQRIADVVKLLDTLAPAERERIVSVLSVPPLVVSLHDAPRAGSGADAVAMH
                     ASMFAALLRNLLGEDREVRVEAVAAAEMPWPPGAGPGYGPGMMGGAGMVGPGMMGPGP
                     GMGPGPLHRGPVFRTEVQLRDGRWARFDSEVPQADAGLPWRLALTLAVLLGAVLVLSF
                     VAVRWVTRPLHVLAQAAEALGKDINRPALPEEGPLEIRQAARAFNTMQARLARYIHDR
                     TRILAAMSHDLKTPITRMRLRADLLEDEELRQRFEADLKEMEAMVTHTLEFMRGLGGN
                     EPRQPVDVMALLESLQSDNEAMGRTMRVEGQASAPYVAVVSLLKRCLGNLIDNAALYG
                     GGRMTVQVDDTLQVLTLRVLDEGPGLPESELEKVFEPFYRLEGSRSRETGGTGLGLTI
                     ARNIAQTYGGDITLRNRPEGGLEAVLTLPREPQAAPLPRAHVH"
     gene            12596..13792
                     /locus_tag="VGA59_01030"
     CDS             12596..13792
                     /locus_tag="VGA59_01030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9718289.1"
                     /translation="MRRWMRRGGLALVALLAAYAVVWLGAYALTDRSGWARAIAWMDS
                     DVDDQHRFPARPVANAPPRFDFKLPGTVERERLAAVWRDIEVESGGQKQMRGFEDFMR
                     DTDTLAFLVLRDDVLLYEGYFNGGSREVPVTSFSVAKSFVSALIGCAVADGRIHSVED
                     PVTRYLPELPARDARYQHIALRHLLSMSSGIRYVERGLPWSDDAITYYGPDLRAVAVS
                     SAIEGPPGQVFHYNNFHPLLLGLVLERTTGVPVARYLEDRLWKRLGMEAPASWSLDSE
                     RSGFEKMESGLNARAIDFAKFARLYLNRGNWQGEQVVPAAWVRESTRRDTATDPAERY
                     QYFWWVNTQVPGRHHFFAAGKHGQYLYVMPERNLVFVRFGRTDPHRRWPATFVRMADR
                     IDAIGR"
     gene            13828..14217
                     /locus_tag="VGA59_01035"
     CDS             13828..14217
                     /locus_tag="VGA59_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX9718290.1"
                     /translation="MSQYVFGKPVSMGFDAAVQRVTEELAKVGFGVLTDIDVQATMRK
                     KLGLEMPPYRILGACNPHMASKAIAAEPQIGALLPCNVVVRQDTQGGIHVEMMDPRAV
                     LQLVDNPDVPALAAEVRQKLEQALNAV"
     gene            complement(14335..14640)
                     /locus_tag="VGA59_01040"
     CDS             complement(14335..14640)
                     /locus_tag="VGA59_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718291.1"
                     /translation="MPQETLPEKPQRASQLLVLAALLLAVTFGIVFTSILAQELLTRY
                     RLVMQAHQQQEMRLMSPDEEKCPGLSRRVPYQHAHRDRLTVGYPAAPGSLPASGRWT"
     gene            complement(14794..15309)
                     /locus_tag="VGA59_01045"
     CDS             complement(14794..15309)
                     /locus_tag="VGA59_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX9718292.1"
                     /translation="MRRPPRFRVAAGLATALAALFAGASTAQQATFQARSITPEAALR
                     AARAALTACGKNGHQVAVAVTDRAGHPLVMLRDRHAGPHTPATATGKAYTALSFKLDT
                     TSFARVTQAGEASSGIRQLPGVVAIGGGWPIEAGGSLVGAIGVSGAPGGEADDACAKA
                     GIAEIRDDLEF"
     gene            complement(15311..>16777)
                     /gene="soxB"
                     /locus_tag="VGA59_01050"
     CDS             complement(15311..>16777)
                     /gene="soxB"
                     /locus_tag="VGA59_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HEX9718293.1"
                     /translation="VPAGSAEAYALTCLDFEQAASRYGKVGGFAHLATLVKRLKANRP
                     GALLLDGGDTWQGSATALWTKGQDMVDAAKLLGVDIMTGHWEFTLGMDRVKEIVEKDF
                     KGKVDFLAQNIKTTDFGDPVFKPYVIREINGVPCAVIGQAFPYTPIANPRYMVADWSF
                     GIQDDNMQKMVDEARAKGAQVVVVLSHNGMDVDLKMASRVTGIDAIMGGHTHDGMPVA
                     SVIANKGGKTLVTNAGSNGKFLAVMDFDVKDRKVRDFRYRLLPVFANMLPADKEMDAL
                     INRIRAPYESKLAEKLAVTEGTLYRRGNFNGSGDQLLLDALMDVQGAEIAFSPGFRWG
                     TSLPAGQPITREWLMDMTATTYSYATVAQMSGEAIKNVLEDVCDNLFNPDPYYQQGGD
                     MVRVGGLEYSCNPLGAMGKRIGDMRLNGKPIEPSRQYKVAGWAPVAEEARTAGNKPVW
                     ELVEQWLKARGGVVTPRKVNMPRLTGGVPNPGYAPPQA"
BASE COUNT         2682 a         5565 c         5684 g         2846 t
ORIGIN      
        1 acggtgatct tgcgcgacat caccgagcgg ctgcaggccg aggaacgaaa catccggctg
       61 gcggcgcggc tggccggcct gctcgactcg gcgatggacg cgatcatcac ggtggacgat
      121 cgccagcgcg tcgtgctgta caaccgcgcg gcggaaaaga tcttcggctg gaccagcgaa
      181 gaggcgatcg gtggcccgct ggagaagttg atgcccgagc gctttcgagg cagccacggg
      241 gcgcacgtgg atcgattcga ccggaccggc accacgtcgc gccgcatggg cgacggcacc
      301 gtgctgtacg ggcggcgcgc gaacggcgag gaatttccga tggaggcttc gatctcgcag
      361 ctggacaccg ccgaaggcaa gctgttcacg gtgatcctgc gcgacatcag cgaacgcatc
      421 cggacccagg aggaattgag cagcttcgcc gccgaggccc acaccatccg tgaaggcgag
      481 aagagccgca tcgcgcgcga gctgcacgac gaactggcgc aggcgctcac ggcgctcaag
      541 atggacacga tctggctgcg cgacaacgcg ccggcccagc cggacaaggc ggtcgccaag
      601 ctggccgaaa tggtcagcat gctggacacg accgtcgccg ccacgaggcg cattgccgcg
      661 gacctgcgtc cgctgctgct cgacgacctg ggcctggcgc cggcgatcga gtggctggcc
      721 cataccttca cgcagcgcac gggcgtgccc tgcgcggtgt cggtggacga ggagctggag
      781 ttgcccgagc cctatgcgac agcggtgttc cgcattgtcc aggagtcgct ggccaacgtg
      841 ggcaagcacg ctcaggcgac cgaagccggg gtggtcatcg cccgcaccgg ccaggccgtc
      901 atgctggaag tgcgcgacaa cggccagggc tttgccaccg cggccccgcg caagccgcat
      961 tcgctgggcc tgatgggcct gcgcgagcgg gcgcagctgc tcaagggcac tgtctccatc
     1021 gacagccggc cgggcaaggg cacccgggtg caggtccgca tcccggtgca ggagatcggg
     1081 gcagccacat gatcaggatc gtgatcgccg acgatcacgc catcgttcgc gaaggcctca
     1141 agcgcatcgt gtccgacgcg ggcgatatgc tcgtggccgg cgaggctgcc gacggcaccg
     1201 aggtcatgaa gcgggtgcgc gagctcgagt tcgacatcct ggtgctcgat ctttccatgc
     1261 ccgggcgcag cggcatggaa ctgatcagga tggttcgcgc ggaaaagccg aagctgcgca
     1321 tcctggttct gagcatgcac caggaactcc agtacgccgt gcgcgccatc aagtcgggcg
     1381 cgagcggcta cctcaccaag gaaagcgcgc ccgcccagct ggaacaggcg atccgcaaga
     1441 tcgccggcgg cggagccttt atcacgccgg aggtggctga gcagttggct ctgggcgcca
     1501 tgccgggcag cgaaacccat ccgcacgaaa cgctgtcgga tcgtgaattc gaggtcttcc
     1561 gtatgctggc cgcgggcgtg tcggtgaccg agatcgcggc ccggctcaag ctctcggtca
     1621 agaccgttag cacgcacaag gccaacctga tgcagaagat gggtttgcag aatgccagcg
     1681 aactgatccg ttacgccatc aggcacggcc tggccgacag cctcgagccc tgacccgtac
     1741 gaggctacag cagccggaag atttcccagc ggctggtgcc ggagaccacg tgtggctcca
     1801 gctccttgcc ccacttctgg tgttccggaa gcgccgccag ccgatcccag acctgattca
     1861 attcggccag gttttcaatc acgtgttcgg actgcacggt ggcttccagc gcgccgatcg
     1921 agccggtcag caagcgggtc tgttccggct tgatgccggc cttggggccg acctcgcgca
     1981 gccatcgctg catcatgtcc accacgttct gcttgtaccc gaacttcgcg tcgatgctcc
     2041 agcgtgcgac catcatgatt cgtctccttg aggaaagtct ctgtggatcc ggtcaaccgc
     2101 aacagtgcgc ccaggcagcg gtcacgccgg gcgcccatgc ggactggcat ttataggcgt
     2161 gtaccggccg ggtttcaagg tgggttgacg ccgctcaagg cggcacggaa attcgcggcc
     2221 tatttttgtt gcaccgcaat gtgtcgcaag gagcggaacc atgaaggaat cactcacgca
     2281 actgctggtc catctggacg cgtcgcccgg cgcctcgcag cgccttgaag tggcgcgtgc
     2341 gatcgcgcaa tcccatggtg cggcggtgac ggcgctgtac gccgtgacgc ccgcgctgct
     2401 ggtcgtgccc ttcgcgcccg aggcggggcc cagtgtcgcc accaccttgc gcgagatcga
     2461 tgacgagcgc cgtgcggccg cgcgagccgc gctcgagcgc tccttggcca cgcccggcgt
     2521 gcacgcggcg tgggctgaag tgcgggacta cccgatcgtg ccggcctttt cgcggcaggc
     2581 gttgtacgcc gacatgctgg tgctgggcca gcatgatcct gcggccccgt cccccggtgt
     2641 gccttccgat ttcgcgcaga ccgtgatggc cgcgagcggc aagccggcca tcgtgctgcc
     2701 ctatgctgga gtgccgtccg ccgtcggcga gaccgcagtc atcgcgtgga aacccacgcg
     2761 cgaggccgcc cgtgccgtga gcgccgccgt gcccatactc cagcgtgccc ggcgcgtgca
     2821 cgtcctttcg tggatggagg agcaagaggg cattgacggc gcccgcctgg acctggacgg
     2881 ctacctcaag ctgcgcggca tcgagccggt ctggcacctc gaggccacgg aacccgacgc
     2941 ggtcggcgag ctgctgctga cccgcgcctt cgatctcgac gccgacctgc tggtgatggg
     3001 ctgttatggc catagccgag ctcgcgagtg ggtgctcggc ggcgcgtccc gcacggtgct
     3061 gggttcaatg actctgccgg tgctgatggc gcattgacgg cgcggcgacg gcggcgcgta
     3121 tcgccgccgc ggccgatttc cgtttccgca ggcccgctac cgcctcctgc ggcatggccg
     3181 gggctccacc cagggcttca cggacgagtt tgacgtgtat caaggcagtc cggccggcgc
     3241 cgggctatcg tcctgaagga tcagtaaggc cgccctggcg ccaagatcga tgcccacaag
     3301 accaaaaccc gactggatac tgattgccaa taccacccgt gcacgggtcc tgcagcagga
     3361 gcccggaaat gcgatggtgg tcctggaaag tttcgtccat ccggccggcc gcggcctgcg
     3421 caccgaaccc ttgatagatg ccaggcagaa agaatgcacg cgcttcgcgc atgaattggc
     3481 ccagttcctg gaacaggagg cgcggcaagg gcacttcggc tgcctgaccc tcttcgcctc
     3541 ggcctccttc ctggaagacc tcagggccag cctgggcaaa gtcaccacga ggctattgtc
     3601 cggaacccac gaactggacc tcaccaatgt cggtgtggcg gagctggagc ggcgcgtttc
     3661 gcacgaactc gcgctgtcgg cgcactagag cggttcgcag gctttcgagg gcaaaaaaaa
     3721 cccgccggcc gttcacacgg gcggcgggct tgcggagact ccggatggcc gctagaacca
     3781 tacccggtct gatgtcgtct cccccatccc gacgattaga ggacgccgga acctcgcgtc
     3841 agggctgggc ccctccgcgg acttgcgtcg agaaagttga gcttcctgcg gcgcgatctc
     3901 actcacttta ggcggcgacc ggggccgcga ctttgatctg ggtcagtcga gtcctccacg
     3961 cgctacaggt ggtaaccgct atcacgccat gcgggtaagc agcttctgga cgtcggccat
     4021 caggggaccg aggtcggtgt ccggcttgcc gtggtcctgc aggtgcgctt ccagctcctg
     4081 ttcgagcatg cacatcgtca tccggacgta agtgctgtca agggccttgc gaacggccga
     4141 aaactgctgt gcgacgtcca ggcagggcga gccttcctcc accatgcgct ggattccgcg
     4201 cagctggccc tcggcgcgtt tcaggcgggt caggatgtcg gcgcggcccg ccgcgtttgt
     4261 gagcgtgggc atcatttcag tcgagctccg gtggcgtccc atacctccac tgcaatggcg
     4321 atgccttctc ccacactttg cgtgacggtg cagaaagcct cgaactggtc cagcgcgcga
     4381 tcgaggtgct ccagcgacga gcccgctacg cctagcgtca gcactgcttt catgctctgc
     4441 acgcgcaggc gtccctgcgc gttgcggccg acgatcgttt cgacgctgca ggtgatcggt
     4501 tcgggcttct gcttgaactt gcgcagcgcg aacagcaggg aatccgacag gcagcttcca
     4561 accgccgctc ccagcaagtg cttcggtgac ggacccttgc cgccaccgag cggggccggc
     4621 tcgtcgacca gcagatccgg cacctcgccg ccgaaccgca cggtgaagct gtagtcgcgc
     4681 tgctgcgtca attcgacgcg aatggtgtct tcagccatgg tgcttctcag ttgccgcagg
     4741 acgcggttgt ttccggcacg cccagcttct tgagcatgaa tccgagaggg cagaacttgg
     4801 tgaaaccgaa ctggaacagg ttcgcgccga cgaaggcagt gaaagcaagc gcccagtggc
     4861 tgacaaaaag cgggctgccg tcgatgccga gggcgagcga gacgaggatg aacacccccg
     4921 cgaaaatgtg gatgtagcgt tctacggtca tggtgcgaac tccaggttga tgaaagaaaa
     4981 tcactgcgcc gagacgttgc cttccggcgg ttcttcggct tcgaagcgct gtcggtacag
     5041 cgcgtaatac aggacgggaa tgaccaccag cgtgagcagg gtggacacca ggatgccgaa
     5101 gatcagcgaa acggccagcc cgttgaagat ggggtcgtcc aggatgaaga agccgccgat
     5161 catcgcggcc aggcccgtga gggcgatcgg ctgcgcacgc actgcggcgg aattcatcac
     5221 ggcgccgcgg aagggaacgc ccctggcgac ctgcagctcg atgaagtcca ccagcaggat
     5281 cgagttgcgt acgatgatgc cggccagcgc gatcatgccg atcatgctgg tggcggtgaa
     5341 ctgggcgccg agcaaggcgt ggcccggcat cacgccgatg atcgtgagcg ggatgggcgc
     5401 catgatcacc agcggcgtga gatagctgcg gaactgggcc accaccagca ggtagatcag
     5461 caccaggccg acggcgtacg ccgctcccat gtcgcggaat gtctcgtagg tgatctgcca
     5521 ctcgccgtcc cacttgatgg cgtaggcccg gtagggatcg gccggctggc ggatccagta
     5581 ctcgcccagc tcgccggtgc ccgccaaagc cgcggcgtcc agcttcgcgc ggatcccgaa
     5641 caggccgtac aggggtgaat cgggcgttcc ttgtcgtgca ctgctgcccg tgcgcaagcc
     5701 aacgtcggcg aacacgtaac tcacgtgctt caggtccttg gtgaagagcg gcttgtcgat
     5761 cgtgccgcgg tccagctgca ccagctccga gagcggaacc atgcgtcccg atgccgagcg
     5821 catcggcagg gccagcaagg catccaggcc gacctggtcc gcgagcggca attgcaggcg
     5881 caccggcacc gggtacttgc tctgcccgtc gtgcaagtag gccgcgtcga cacccgacag
     5941 cgccgaatgc acggtctgcg ccaccgccga caccggaatg ccgagcgact ccgcgcgctg
     6001 cctgcgaacc gtcaggtgaa cctgctgcgc atcttccttg agcgagctat ccacgcccac
     6061 gatatccggc gtgccgctga aagccgcggc gatgcgctgc gtaagccgtt ggcgcccggc
     6121 ttcgtcggga ccgtagacct cggccacgag cgggctcatg accggcggcc ccggcggaac
     6181 ttcaacgatc ttcacccggg cctggtggcg cgcgccgatt tcctcgaggg cagggcgcag
     6241 gcgttgcgcg atcgcatggc tttgctcggt acgctggtgc ttgccggcca ggttcacctg
     6301 cagatccccc tgttcggctt cggcgcgcag gtagtactgg cgcaccaggc cgttgaaggt
     6361 gatcgggctg gcggtgccgg catagccttg caggtcgcgc acttcgggct gccgcgcgag
     6421 ccacccgccc atttcgtgca gtgcggcggc cgtgttctcc agcggcgtgc ccgccggcat
     6481 ttccaccacc acctggaact cgctcttgtt gtcgaagggc agcatcttga gcaccaccca
     6541 ttggacgacg accagtccaa ccgacagcgc cagggcggcg aggattcccg ccagcaggag
     6601 ccagcgcttg cgcgcactcg acagcagcgg tgtgagcagc cgcgtgaaca gggtctgcac
     6661 gcgtcccgtc aagccacccg cgtgccccgg cgtgtggctg tggccgtgat ggggcttcat
     6721 cagcttcagc gccagccagg gcgtcacggt gaacgcgatc gccagcgaaa tcgccatgcc
     6781 caggctggag ttgatcggga tcgggctcat gtacgggccc atcaggcccg acacgaatgc
     6841 catgggcagc agcgccgcga tcacggtgag ggtcgccaga atcgtcgggc cgcccacttc
     6901 gtccaccgcg ccgggaacga tctcgcgcaa cgggcgtccg gggtgcaact gctggtggcg
     6961 atggatgttc tcgacgacga cgatggcgtc gtccaccagg atgccgatgg agaagatcag
     7021 cgcgaacagc gacacccggt tgagggtgaa cccccaggcc caggacgcga acagggtggc
     7081 ggtgagcgtg aggatcaccg ccgcgccgac gatggcggct tcgcgccggc ccagggccaa
     7141 acccaccagc acgatcacgg atgcggtcgc gaatccgagc ttctgcatca gcttgttggc
     7201 cttttccgcg gcggtctcgc cgtagtcgcg cgtcaccgtg gtctcgatgt tggccgggat
     7261 caccgtgttg cgcagcgact cgacgcgctg gcgcacggca ctggccacgt ccaccgcgtt
     7321 ctcgcccggt ttcttggtca ccgtgatcgt gacggcggga aagacctgtc ccgtctgggc
     7381 cgctgccgcg gcgtccttgt cgcccgcggc gaaagccgcg cccggtgtca tccagacata
     7441 acgctgcggc aggcgggcac cggtttcgat gcgcgcgacc tcgcgcaggt aaagcggctt
     7501 gccctggctg acgccgacca ccagcgaggc gatgtcctct tccgatcgca gaaactcgcc
     7561 ggtctccacg gtgagcatgc gggcggtcgc atcggaggcg tcgagcacgg cgcccgaggg
     7621 catgcccagg ttcgcggctg ccagcgtggc cttcaggccc agcacgtcga cgccgcggtc
     7681 gcgcatgcgc accgggtcga gccagacgtg cacggcgcgg cccggcccgc cgatggtctg
     7741 cacctcgcgc gttccggcca cgctcttgag ttcggcttcg agcgtgcgcg cgacccgctc
     7801 gaggtcgttc gctgcaagca catcgcggct ccacaacgtc acggcgagca cgggcacgtc
     7861 gtcgatgccc ttgggcttga cgatcggcgg cagcgcgccc aggtcgcgcg gcagccagtc
     7921 ctggttggcg ttgagcacgt cgtacaggcg caccagcgcc tcggtccgcg gcacgccgac
     7981 cttgaactgc accgtcagca cggccagccc ggggcgggcc acggaatagg tgtgctcaat
     8041 gccggcgatc tgcgagagca cctgttcggc cggccgcgcc accatgttct gcacgtcggc
     8101 gctgctcgct ccgggaaaag cgatcagcac gttggccatg gtcacgttga tctgcggctc
     8161 ttcctcgcgc ggcgtgacga gcacggcgaa gagccccagc agcagcgcca cgatcgccag
     8221 caagggcgtg atggcgttgt cctggaatgc cgcggcgatg cgcccggaaa cgcccagtcg
     8281 ctggatgggc tgagacgagg cggaggtctg gtcctgcgtc atatccgtct cagggtgcaa
     8341 gcgacgggcg cgcgccggca aggctggccc tgaccgggtc cagcgcgacg gagtcggcgg
     8401 cattcagtcc ggccaggatt tcaacccccg cagcgccgtg atcggctccc agtcgcaccg
     8461 ccttgagcac gaagccggcc ccgtcgggcg acgccacgta aacggccgtc aattcgcccc
     8521 gccgcagcac ggcgcttgcc ggtaccacca ggcgccgctg cgggctggcg gcaaaccgaa
     8581 cgtgcacctg ctggccaggc gccacgctgg ccgccgcggc cgcgggcagc tcgagacgcc
     8641 actccagcgt ttgcgacacg ggatcggccg caggcagcat cgccctcacc gtcggggtga
     8701 tccactgcgc gccgcctttg ccattgggaa tctgcacttc cacctcgacg gcggatcgtg
     8761 cggcggcggc gcgcgaagcc ggcacctgga ccacggcccg cagcggcaag ggtgcgtaca
     8821 gggtcagcag tggcttgccc ggcaccgcca ggtctccggg ctgggcgagg gtttccagca
     8881 cccacccgtc gtacggcgcc gtaacccgag tgaattcctg cgcgatcgcg gactggcgct
     8941 ggcccgccgt ggcctggtcg cgtgaggcct gcgctgactt gaaggtggcc tcggccgtgt
     9001 cgagcgccgc tttgctgacg aagcccttcg cctgcagctc ccgcgtacgc tgcacctggg
     9061 cctgcgcatt gcgcaactcg gcttcggcct gggccgtctg ggcccggctg cgttgcagcc
     9121 cggcctggct ctcgcggtcg tcgatgacgg cgagcagctg gccggtgcgc acccggtctc
     9181 ccgccttgat ggcgagctgg ctgatgcgcc cggatgtttg cgctgcgacg gtgctctgct
     9241 tgaccggttc aaccacgcca tcgagctcga acccgacggg gaccgcctgc gcactggctt
     9301 tcacgaccgg cacgacgatc gccggcccgc cccacgccgc cgcggcagcc agcagtccgg
     9361 ccagcgaagc gaggcgcagg actcccgcaa cggccgtgac gagtgaaatt gaagagtgga
     9421 tcatgtgatt ccggttgctc gatgcgatta tcatagcata ctcccaccag tatgcgtttg
     9481 tccttgattc gcatcaagca gcgccggtat cggccagcaa tttcctccct ggacgcttac
     9541 gtgtcgcgcg ccggttcagc gacccgggaa attccgcccc gcgcggggac ccccaattcc
     9601 catggggccg cggtcggcga ggacccgctg ctcgggcgtg agaacggcgt agagatcctt
     9661 cagtgccgcc gtgttggccg cccagctggc gtcgtgttgc ttgatcatgg cgtcgcgctg
     9721 cgcggtccac tcggtgttgc ccggcgcctg gttttgcatc tgcgcgtgca tctgggtgcg
     9781 cagggcctgc atcgtggtcg cctgttgcct gagcgcggtc tcgtacgctt tccacgccgt
     9841 ttcctgcgcg ggcgcgatct tcagcagcac tttcagttcg gccgcacggc cggcggccac
     9901 ggcctccatg tccgcgccgc ccatccagcc gcgcatccca taacccatgt ggcccgcgcc
     9961 cggtcccatg ccggctccca tgccccagcc gtgtcccatg cccgggccca tgccccatcc
    10021 gggccccatg ccccaggagc cgccgtcggg gtgggcatac acggcgccgg ccagcgccag
    10081 cgacaatgct gccgcggcgc ccgcgatgat cttggtggtg aatttcatag tgcgtttcct
    10141 gtcaatggtc ggttaaaacg gatcgctgcg tctggccgca tcgaccggtt ggaggcaagt
    10201 ccctcgcaac tgacttcatt tcagcgcgtc cttgtaagcg cggaatgtca agccggcgcc
    10261 tttttgtcgc gcactgcgac aggcggcgcg cgtgacatgc tgtgttactt tgcatggtgc
    10321 gcgccgtgcc gcggcgaagg ctgtaatacg aaccgatgga ccgatccgac catatcctga
    10381 tcgtcgatga cgacgccgaa atccgcagcc tgctcagccg ctacctgcag aagaacggcc
    10441 tgcgggcgac cgcggtcgcg gacgggcgcg ccatgtggca ggcattggac aggggcagct
    10501 tcgacctgat cgtgctggac ctgatgctcc ccggtgacga tggcctcacg ctgtgccgca
    10561 cgctgcgggc gcgctccgac atgccagtca tcatgctcac ggcccgcggc gaagaaaccg
    10621 accgcatcgt gggcctggag atgggcgcgg acgattacct cgccaagcct ttcagcgcgc
    10681 gcgaactgct ggcgcgcatc aaggtcatcc tgcggcgcgc gcgcagcctg ccgcccaatt
    10741 tgcaaccgga ggaagagcgc gagatccact tcggcgactg gagtctggac acggtgcatc
    10801 gccacctggt ttcgccggcg ggcgtggtga cgcccatcgg gggcgcggag taccgtctgc
    10861 tgcggatctt cctcgagcac ccgaaccgtg tgctcagccg cgaccagctg gtggagatga
    10921 cacagggcaa ggaggccgac gcgctggacc gcagcatcga cgtgcaggtc agccggctgc
    10981 ggcgccggct tggcgacggc tcgcgcgatc ccggcatgat caagaccgtg cgcggcgagg
    11041 gctacgtgtt gtccgtcccg gtgaacaagg cctcctcctg atgcatgcct ggacgcgctg
    11101 gctgccacgg tcgctgtttg ggcggctgat gctggtgctg gccagcggct tgctgctcgc
    11161 ccagctgctc agcgccgcga tcaacctggc tgagcgtgac cggctgctgc tgcgggcccg
    11221 cggcacgcag caggcgcagc gcatcgccga cgtggtcaag ttgctggaca cgctggcccc
    11281 ggcggagcgc gagcggatcg tcagcgtgct cagcgttccg cccctcgtcg tgtccttgca
    11341 cgacgccccg cgggcggggt cgggtgcgga cgcggtcgca atgcatgcaa gcatgtttgc
    11401 cgccctgctg cgcaacttgc tgggcgagga ccgcgaagtg cgcgtcgagg ccgtcgccgc
    11461 cgccgaaatg ccgtggccgc cgggcgctgg ccccggatac ggaccgggaa tgatgggggg
    11521 tgccggcatg gtggggcccg gaatgatggg accgggcccg ggcatgggcc cggggcccct
    11581 gcatcgcggc cccgtctttc gcacggaagt ccagctgcgc gacggccgct gggcgcgctt
    11641 cgacagcgag gtgccgcaag ccgatgccgg gctgccgtgg cggctcgcgc tgacgcttgc
    11701 cgtgctgctg ggcgccgtcc tcgtgctgtc gttcgtggcg gtacgctggg tcacacggcc
    11761 tctgcacgtg ctggcgcaag ccgccgaggc gttgggcaag gacatcaacc gtcccgcgct
    11821 gcccgaggag ggcccgctgg agatccggca ggccgcccgg gcgttcaaca ccatgcaggc
    11881 gcgcctggcc cgctatatcc atgaccgcac ccgcatcctc gcggcgatgt cgcacgacct
    11941 gaagacgccg atcacgcgca tgcggctgcg ggccgaccta ctggaggacg aagagctgcg
    12001 ccagcgcttc gaagcggacc tgaaggagat ggaggcgatg gtcacgcaca cgctggagtt
    12061 catgcgcggg ctgggtggca acgaaccgcg gcagccggtg gacgtgatgg cgttgctcga
    12121 gagcctgcag tccgacaacg aggcgatggg ccgcacgatg cgggtggaag gacaggccag
    12181 cgcgccgtat gtggcggtgg tatcgctgct caagcgctgc ctgggcaacc tcatcgacaa
    12241 tgccgcgctt tatggcggcg gccgcatgac ggtgcaggtc gacgacacgc tgcaggtgct
    12301 gaccctgcgc gtgctcgacg agggtccggg gctgccagaa tccgaattgg agaaggtctt
    12361 cgagccgttc taccggctcg agggctcgcg cagccgcgag accggtggca ccggcctggg
    12421 cctcacgatc gcgcgcaaca tcgcgcaaac gtacggcggt gacatcacgc tgcgcaatcg
    12481 cccggagggc ggactggagg cggtgctcac cttgccgcgg gagccgcagg cagcgccctt
    12541 gccgcgcgca catgttcatt agactcgagc atcgggaccc aatgaggtga cggccatgcg
    12601 acgctggatg cgaaggggag ggctggcgct cgtcgctctg ctggcggcgt acgcagtggt
    12661 gtggctcggc gcgtatgcgc tcacggaccg atccggctgg gcccgtgcga tcgcctggat
    12721 ggactccgac gtggacgacc agcaccgctt cccggcccgg ccggtcgcga acgcgccgcc
    12781 gcgcttcgat ttcaaactgc cgggcactgt cgagcgcgaa cgtctcgcag cggtgtggcg
    12841 cgacatcgag gtcgaatccg gcgggcaaaa acagatgcgc ggtttcgagg acttcatgcg
    12901 ggacacggat acgctcgcct tcctggtgtt gcgcgatgac gtgctgctgt acgagggcta
    12961 cttcaacggc gggtcgcgcg aagtgcctgt gacttcgttc tccgtcgcca aatcgtttgt
    13021 ctcggccctc atcggatgcg ccgtcgccga cgggcgcatt cacagcgttg aggatccggt
    13081 cactcgctac cttcccgaac tgccggcgcg ggatgcccgc taccagcaca tcgccctgcg
    13141 ccacctgctg agcatgtcgt cggggatccg ctacgtggag cgcggcttgc cgtggagcga
    13201 cgacgcgatc acgtattacg gccccgacct gcgcgcggtc gcggtctcca gcgctatcga
    13261 gggcccgccc ggccaggttt tccactacaa caacttccac cccctgctgc ttggcctggt
    13321 gctggagcga acgaccggcg ttccggttgc tcgctacctg gaggaccgtc tctggaagcg
    13381 gctgggcatg gaagcgccgg cttcctggag cctggacagc gagcgcagcg ggttcgaaaa
    13441 aatggaaagc ggactgaacg cgcgcgcgat cgatttcgcg aagttcgccc ggctttatct
    13501 gaaccgcggc aactggcagg gcgagcaggt cgtacccgcg gcctgggtgc gggagtcgac
    13561 ccgccgcgac acggcgacgg acccggccga gcgctatcaa tacttttggt gggtcaacac
    13621 acaggtgccg gggcgccacc acttcttcgc cgccggcaag catgggcagt acctgtacgt
    13681 gatgcccgag cgcaacctgg tgttcgtgcg cttcggccgc accgatccgc accggcgctg
    13741 gccggccacg ttcgtgcgga tggccgatcg tatcgatgcg atcgggcgct gaatttcatt
    13801 gcacattttt ccgttcaagg aggtgatatg agtcagtatg tattcgggaa gcctgtttcg
    13861 atgggcttcg acgcggcggt gcagcgggtc accgaggaac ttgcgaaagt cggcttcggc
    13921 gttctgaccg acatcgacgt acaggccacc atgcgcaaga agctggggct ggagatgccg
    13981 ccttaccgca tccttggcgc ttgcaacccg cacatggcca gcaaggcgat tgcggccgaa
    14041 ccgcagatcg gtgccttgct gccttgcaac gtggtggtca ggcaggacac gcagggcggg
    14101 atccacgtcg agatgatgga tccccgcgcg gtgcttcaac ttgtcgacaa cccggatgtg
    14161 cctgcgctgg cggccgaggt gcgacagaag ctggaacagg ccctcaacgc ggtttgaatc
    14221 gtccgggccg cgggcttcgg ttgaagcggt ctgaacgcga cgcccgcagt caggcgtcga
    14281 tgcgcccgag cacgctttgc gccgtttggc ggcgggcccg atcggctgcg atgctcatgt
    14341 ccacctgccg gatgccggca gactgccggg agcggccgga tagccgaccg tgagccggtc
    14401 acggtgggcg tgctgatacg gcacgcgtct tgacaggccc gggcactttt cctcgtccgg
    14461 gctcatcaga cgcatttcct gctgctgatg agcctgcatg accaagcggt accgtgtcag
    14521 cagttcctgc gcgaggatgg aggtgaagac gatcccgaac gtgacggcga gcagcagggc
    14581 tgcaagcacg agcagttggg acgcgcgttg cggtttttcc ggcaaagttt cttgtggcat
    14641 tccaacgcct ttctggtcgg gttagcccta ccttgcctga gtgcgtgcgc gcgtggcgct
    14701 ctgcagtgga ttatttcaaa ctgcaacgca cggctggggt cgcatgtgat caactcattc
    14761 gttgcgctgt ccccagacga acagcacttg gtgtcagaac tccaggtcgt cgcggatctc
    14821 ggcgatgccg gctttggcgc aggcatcgtc cgcttccccg ccaggggctc cggaaacgcc
    14881 gatggcgcca acgagtgacc cgcccgcttc gatcggccag ccgccgccga tggcgacgac
    14941 gcccggcagc tgccggatgc ccgaggacgc ttcgccggcc tgcgtcacgc gcgcaaacga
    15001 ggtcgtatcc agcttgaagc tcagcgcggt gtatgccttg cctgtcgccg tcgcgggggt
    15061 atgcggtccg gcatggcgat cgcgcagcat caccagggga tggccggcgc ggtcggtgac
    15121 ggccacggcg acctggtgcc cgttcttgcc gcacgcggtc aatgcggcgc gcgcggcacg
    15181 cagtgcggct tcgggtgtga tcgagcgcgc ctgaaaggtg gcctgctgtg ctgtcgatgc
    15241 gccagcgaac agggcggcga gtgcggtcgc cagaccggcg gcaacgcgaa atcggggcgg
    15301 gcgccgcatg ctatgcctgg ggcggggcgt aaccggggtt gggcacgccg ccggtgagcc
    15361 gcggcatgtt gaccttgcgc ggcgtgacga cgccgcccct ggctttgagc cattgctcga
    15421 ccaattccca caccggcttg ttgcccgccg tgcgcgcttc ctccgccacc ggtgcccagc
    15481 cggccacctt gtactggcgc gaaggctcga tcggtttgcc gttcagtcgc atgtcgccaa
    15541 tgcgcttgcc catcgcgccg agcgggttgc agctgtattc gagcccgccg acgcggacca
    15601 tgtcgccgcc ttgctggtaa tacgggtcgg ggttgaagag gttgtcgcag acgtcttcca
    15661 atacgttctt gatcgcctcg ccgctcatct gcgcgacggt ggcgtacgag tacgtcgttg
    15721 cggtcatgtc catcagccac tcgcgcgtga tcggctggcc tgccggcagc gacgtgcccc
    15781 agcggaatcc cggcgagaag gcgatctcgg cgccttgcac atccatcagg gcgtccagca
    15841 gcaactggtc gccgctgcca ttgaaattgc cgcggcggta cagcgtgcct tcggtgacgg
    15901 cgagtttttc ggccagcttc gattcgtagg gtgcgcggat gcggttgatc agcgcatcca
    15961 tttccttgtc cgccggcagc atgttggcga acacgggcag caggcggtaa cggaagtccc
    16021 tcactttccg atccttcacg tcgaaatcca tcacggcaag gaacttgccg ttcgagcccg
    16081 cgttcgtcac cagcgtcttg ccgcccttgt tggcgatgac gctggccacg ggcatgccat
    16141 cgtgcgtgtg gccgcccatg atcgcatcga tgccggtcac ccggcttgcc atcttcaggt
    16201 ccacatccat cccgttgtgg ctgagcacga caaccacttg cgcgcccttg gcgcgggctt
    16261 cgtccaccat cttctgcatg ttgtcgtcct ggatgccgaa gctccagtcc gccaccatgt
    16321 aacgcgggtt ggcgatgggc gtgtacggaa atgcctggcc gatgacggcg cacggcacgc
    16381 cgttgatctc gcggatgaca tacggcttga agaccgggtc gccgaagtcg gtggtcttga
    16441 tgttctgcgc caggaagtcg accttgccct tgaagtcctt ttcgacgatt tccttgacgc
    16501 ggtccatgcc gagcgtgaat tcccagtgcc cggtcatgat gtcgacgccg agtagcttgg
    16561 ccgcgtcgac catgtcctgg cctttcgtcc acaaagccgt ggccgagccc tgccacgtgt
    16621 cgccgccatc cagcaggagg gcgccgggac ggttcgcctt caggcgcttg accagcgtag
    16681 ccaggtgggc gaacccgccg accttgccgt aacgactggc cgcctgttcg aagtccaggc
    16741 aggtgagggc gtacgcctcg gcggagccgg ccggcac
//
LOCUS       DASWIY010000028         5716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_391866, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000028 DASWIY010000000
VERSION     DASWIY010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391866"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..824)
                     /locus_tag="VGA59_01055"
     CDS             complement(105..824)
                     /locus_tag="VGA59_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylmalonate decarboxylase"
                     /protein_id="HEX9718294.1"
                     /translation="MMRSSPCAGLIVPPAAGLVPQDGPMLYGDRVKFIARGLGIGAVS
                     PDGFNPVIDSILDKARELRGEGAEVISLMGTSISFYRGPEFTESLRAAMQEATGVPCT
                     TMSHAIVAALRRLGIQRVAVATSYIDELNSRLVDYLGTAGFTVTAIRGMSITGVKEVG
                     EVTTQALVDLSMSVYREDTSAQGVFISCGGLLTLDAIKQLEDRLGLPATASSPAGFWD
                     VVRLGGWDASSSGFGRLFEAG"
     gene            complement(821..1798)
                     /locus_tag="VGA59_01060"
     CDS             complement(821..1798)
                     /locus_tag="VGA59_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9718295.1"
                     /translation="MTFSSRALRRRTLLACALAVAAPLAAAQTYPTKPIRLLVGYAAG
                     GGVDAMARMLSARLPALLGQQVIVENRAGASGTIAADVVAKSAPDGYTLLVSESGLLI
                     ATYLQPRANLDPLKAFAPVAGLFLAPLMIIAGNTVPATTPKELIRLLKSNPGKYSYAT
                     SGVGTVHHLGFEMMKAQTGTFVLHIPYRGAAQIVPDVISGQVPLGVVSATAGMVQAKA
                     GRLRAVAMMSTAKLAGAENVPALADALPGFDMAPRLFLLAPAGTPAPVVEKLNDAVRT
                     VLASADLAQTAALQGAIPAYMASGPLTADLARESADWVKIIKAQKISVE"
     gene            complement(1834..2988)
                     /locus_tag="VGA59_01065"
     CDS             complement(1834..2988)
                     /locus_tag="VGA59_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX9718296.1"
                     /translation="MQISSVRATPLNLPVSVSVSGRTKSTSLSLCIVDVELDDGRIGT
                     GMTAITEEEVVGSIVNDIAGHALVGMDPLRHEQIWERLYWLLAPRGQSGYASHAIAAI
                     DLALWDLKGQILGQSCWRLLGGARSQVPVYATFGFAFFEREELADAARSWVDRGFRRL
                     KMTVGHHALQRRDEPRPLADVIAEDVKRLEAVRDAVGPDVQIYIDANCSLDYFHALSL
                     AQRIEHLGVTFFEEPVSQNDIPNLVKLRAKTSIPLAAGQNEGLASRFRDMLVAQAVDV
                     VQPNACISGGFTQCLRIAGMAAAFNTQFANGGAWPHHNMHLHAGLANGTLVEYHSVAV
                     ECCKLVFDDLPEPSNGVLTLPDTPGLGFKPNRERIAKLARRPGSRGAGKA"
     gene            3304..3453
                     /locus_tag="VGA59_01070"
     CDS             3304..3453
                     /locus_tag="VGA59_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718297.1"
                     /translation="MKILNLIHVMVAQAFYKWAMREINPLHPDLPQIVLRQKELDDKA
                     QRIFA"
     gene            complement(3582..3770)
                     /locus_tag="VGA59_01075"
     CDS             complement(3582..3770)
                     /locus_tag="VGA59_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718298.1"
                     /translation="MKALYLIIPFGLAIAGCTTYPVVVSAPAPAPAVVATVPALSDVD
                     GDGVADIYDRYPHNSLMR"
     gene            4090..4509
                     /locus_tag="VGA59_01080"
     CDS             4090..4509
                     /locus_tag="VGA59_01080"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9718299.1"
                     /translation="MTREVLYTHRVAFGDCDPARIVWFPNFFRWIDSASRHFFVECGV
                     PPWHETEETLGIIGTPLVDTHATFAKSASYGDELVIHTTIPEWRNKSFVQRYRVMRGE
                     DLIMECDEVRIFAARSLQDPKGIRAVPIPESIRRLCS"
     gene            complement(4511..>5716)
                     /locus_tag="VGA59_01085"
     CDS             complement(4511..>5716)
                     /locus_tag="VGA59_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9718300.1"
                     /translation="ESKMLLAVRGGGHNGGGLGVCEDGMVIDLSALRGIRVDPKAHTV
                     RVEGGCKWGDVDHATHAFGMATPAGIISTTGVGGLTLGGGIGHLSRRYGLTIDNLLAV
                     DVVLADGRFVTASANENEDLFWAVRGGGGNFGVVTSFEFRLHSLSTVVAGPTLWPLER
                     AAEVMKWYRDFIPKAPAELNGFFAFLTVPPGPPFPEHLHLKKMCGVVWCYAGGAEQAD
                     KALEPVRALNPALYGVGPMPYPMLQSAFDGLYPPGHQWYWRADFVNELPDEAIALHVE
                     HGSRMPTMQSSMHLYPIDGAVHRVGRKDTPFDYRDAKWASVIVGVDPDPANRELITTW
                     CKDYWEALHPYSAGGAYVNFMMDEGQERVQATYREHYPKLAAIKQKYDPGNLFRVNQN
                     IKPAGANAP"
BASE COUNT          999 a         1885 c         1828 g         1004 t
ORIGIN      
        1 ttgccttgct gtcaagaaac actgactcgc attctgtgaa gccagccaac attcccccgg
       61 gatccattcg gcaggcattg acccgacgcg aatggttgac ctcctcaacc ggcctcgaac
      121 aatcttccga acccgctcga gctcgcatcc cagccgccca gccgcaccac gtcccagaag
      181 ccggccggtg aactggccgt ggcgggaagg cccagccggt cctcgagctg cttgatcgcg
      241 tccagcgtga gaaggccgcc gcaggaaatg aaaacgccct gcgccgaggt gtcttcccgg
      301 tacaccgaca tcgacaagtc cacgagggct tgcgtcgtca cttcaccgac ctctttcacg
      361 ccggtaatcg acatcccgcg aatcgccgtc acggtgaatc ctgccgtgcc gaggtagtcc
      421 accagtcgcg agttcagttc gtcgatgtac gaagttgcca ccgccacccg ctggatgcca
      481 aggcgccgca gcgccgcgac gatggcatgg ctcatcgtcg tgcacggcac accggtggct
      541 tcctgcattg ctgcccgcaa cgactcggtg aactccgggc cccggtagaa actgatggaa
      601 gtgcccatga gcgagatcac ctcggctccc tcgccgcgca attcgcgggc cttgtccagg
      661 atgctgtcga tgacggggtt gaatccatcg ggtgagacgg cgccgatgcc gagcccgcgc
      721 gcgatgaatt tcacgcggtc gccgtagagc atgggcccgt cctgcggcac cagtccagcg
      781 gctggcggaa cgatcagccc ggcgcaggga gaagagcgca tcattccacc gatatcttct
      841 gtgctttgat gatcttcacc caatccgccg actcgcgcgc caggtcggcc gtcaagggtc
      901 cggaagccat gtacgcggga atcgcgccct gcagcgccgc ggtctgcgcg aggtcggcgc
      961 tggccagcac ggtgcgcacg gcgtcgttca gtttttccac cacgggcgcc ggcgtgccgg
     1021 ccggcgccag caggaagagc cgcggcgcca tgtcgaagcc cggcagcgcg tcggcaagcg
     1081 ccggaacgtt ttcggcaccg gcgagcttgg cggtgctcat catggccacg gcgcggagcc
     1141 tgccggcctt ggcctggacc atccccgctg tggcgctcac cacgccgagc gggacctggc
     1201 cgctgatcac gtccggcacg atctgcgccg cgccccgata ggggatatga aggacgaagg
     1261 tgccggtctg cgccttcatc atttcgaagc cgaggtgatg caccgtgcct accccggaag
     1321 tggcatagga gtacttgccg ggattggatt tcagcagccg gatcagttcc ttgggcgtgg
     1381 ttgcgggcac ggtgttgccc gcgatgatca tgagcggagc gagaaacaat ccggccaccg
     1441 gcgcgaaggc cttcaggggg tccaggttcg cacggggctg caggtaagtt gcgatcagca
     1501 ggccgctttc actcaccagc agcgtgtagc cgtccggcgc cgacttcgcc accacgtcgg
     1561 cagcgatcgt gcccgacgca ccggctcggt tttccacaat gacctgctgc cccagcagcg
     1621 cgggcagccg cgccgacagc atgcgggcca tcgcgtccac accgcctccg gccgcgtagc
     1681 cgaccagcaa gcggatcggc ttggtgggat aggtctgggc ggcggccagg ggcgctgcga
     1741 cggcgagcgc gcaggccagc agggttcggc gccgaagcgc acgggaagag aatgtcatgt
     1801 catgtctcct atctttgttg tcagtcacac ctatcatgcc ttgcctgcgc cgcgcgaacc
     1861 cggccggcgc gccagtttgg caatacgctc gcggttgggc ttgaagccca ggccgggcgt
     1921 gtcgggcaaa gtcagcacgc cgttgctggg ttctggcaag tcgtcgaaca ccagcttgca
     1981 gcactcgacg gcaacggagt ggtactcaac cagcgttcca ttggccaggc cggcgtgcag
     2041 gtgcatgttg tggtggggcc aggccccgcc gttggcgaac tgggtattga acgccgccgc
     2101 catccccgcg atgcgcaggc actgcgtgaa gccgcctgaa atgcaggcgt tgggttgaac
     2161 cacgtccacc gcctgcgcaa ccagcatgtc gcggaaccgg ctcgccaatc cttcgttctg
     2221 ccccgccgcg agcggaatgg aggtcttggc ccgcagcttg accaaattcg gaatgtcgtt
     2281 ctgcgacacc ggctcttcga agaaggtcac gcccaggtgc tcgatccgtt gcgccaggga
     2341 caatgcatgg aagtagtcga ggctgcagtt cgcgtcgatg tagatctgga cgtcgggtcc
     2401 caccgcatcg cgaaccgctt cgagccgctt gacgtcctcc gcgatcacgt cggccagcgg
     2461 cctgggctcg tcccgccgct ggagcgcgtg gtggcccacg gtcattttca gccgcctgaa
     2521 gccgcgatcg acccagcttc gggctgcgtc ggccagttcc tcccgctcga aaaaagcgaa
     2581 gccgaaggtg gcgtagacgg gcacctggga acgggcaccg ccgagcagcc gccagcagga
     2641 ctggcccagg atctggccct tcaggtccca cagcgcgaga tcgatggccg cgatggcgtg
     2701 gctggcgtag cccgactggc cgcggggcgc gagcagccag tagagccgct cccatatctg
     2761 ctcgtggcgc agcggatcca tgccgaccag cgcatggccg gcgatgtcgt tgacgatgga
     2821 gccgacaact tcttcctcgg tgatggccgt catgccggtg ccgatccggc catcgtcgag
     2881 ttcgacgtcg acgatgcaca gcgagagcga agtggatttg gtgcggcccg aaacgctcac
     2941 ggaaaccggg agattcagcg gcgtggcccg aacggatgag atctgcatga gggcgccctg
     3001 tcagctgggg ttcatgcggg cattatgggc gcccggaacg gcgtgttccg gccgaggcgg
     3061 gctggacgcc ttctctgctc cggaaggcaa ctatcttttc gagcgcttga gtatgtaata
     3121 gcgcgtactc agccaggagt cgagcaggcc cacggccctg cgaagcccgc gcccaccggg
     3181 gcgccccact cgacagtagt gctggacgtt ggctccgtcc gacaggccct gtcggttttt
     3241 tcgcccgaat ggacgagcca tgcgcctacg ttccgatacg cggtgttacg cagaatgact
     3301 tgaatgaaga tactcaacct catccatgtc atggtcgcgc aggccttcta caagtgggcg
     3361 atgcgtgaaa tcaaccccct gcatcccgac ctgccccaga tcgtgctgag gcaaaaggag
     3421 ctcgacgaca aggcccagcg catcttcgcc tgaggtgccc ctggcggggc ggcatcgtat
     3481 tgctcggcct cgtggtcgcg actggcatgc tgtcgcggtg gaggccgagg cgtagtggcc
     3541 gctcaggcgc ccgttgcggc accttggcct actcggctgc gtcaacgcat cagcgagttg
     3601 tgcgggtagc ggtcgtagat gtccgccacg ccgtcgccgt ccacgtcgga cagcgccggg
     3661 accgtggcta ccacggccgg cgccggagcc ggagccgaaa ccaccacggg gtaggtagta
     3721 cagccggcga tcgccaggcc gaacggaatg atcaggtaca acgctttcat gaggatctcc
     3781 ttaaacccac aaaaactgaa tgcgggataa ggaatagttt gagacgctgc ccgagattcg
     3841 tggcggcgat tcgtttctgc ggtgtctcga tgtcaagcag cgatacggtg cctctgcgct
     3901 atgccggaat ttgatatcac ttagtcatgt tatcttattg atttatgact cagcggcctt
     3961 gacacttgtg ttttcgcgag ccggaggcca gcgcttgtcc aaccccatca aggtgatcgc
     4021 gactcgaggc gtttcagtgc gatcatcaac gaacgcgaga tcgctgcgga ttgaccagga
     4081 ggcgaacgga tgaccaggga agtgctctat acccaccgcg tcgcattcgg cgactgcgac
     4141 cccgcgcgca tcgtctggtt tccgaatttc ttccgctgga tcgactcggc ttcgcgccac
     4201 ttcttcgtcg aatgcggcgt gccgccctgg cacgaaaccg aggaaacgct gggcatcatc
     4261 ggcacgcccc tggtggacac gcacgccacg ttcgccaaat cggccagcta cggcgacgag
     4321 ctggtgatcc acaccaccat tcccgagtgg cgcaacaaga gcttcgtgca gcgctaccgc
     4381 gtgatgcgcg gcgaagacct catcatggaa tgcgatgagg tgcgcatctt cgccgcgcgc
     4441 agccttcagg accccaaggg gatccgcgcc gtgccgatcc ccgagtcgat ccgccggctc
     4501 tgctcctgaa ctacggggcg ttcgcgccag cgggtttgat gttctggttg acgcggaaca
     4561 ggttacccgg gtcgtacttt tgcttgatgg cagcaagctt cggatagtgc tcgcggtaag
     4621 tggcctggac gcgctcctgc ccttcgtcca tcatgaagtt cacgtaggcg cctcccgccg
     4681 agtacgggtg cagtgcttcc cagtagtcct tgcaccaggt ggtgatgagc tcgcggttgg
     4741 ccggatccgg atcgaccccg acgatgaccg aggcccactt ggcgtcccgg tagtcgaagg
     4801 gcgtgtcctt gcgaccgaca cggtggaccg caccatcgat cgggtacagg tgcatcgagg
     4861 actgcatcgt cggcatcctg gatccatgtt ccacatgcag tgcgatcgcc tcatccggca
     4921 gctcgttcac gaagtcggcg cgccagtacc actggtgccc cggcggatac agcccgtcga
     4981 aagcgctttg cagcatggga tagggcattg gccctacgcc atacagcgcc ggattcaggg
     5041 ctcgcacagg ctccagtgcc ttgtccgcct gctccgcacc gcccgcgtag caccacacca
     5101 cgccgcacat cttcttcagg tgcaggtgct ccgggaacgg cggaccgggc ggcaccgtga
     5161 ggaaggcgaa gaagccgttg agctccgccg gcgccttcgg gatgaaatcg cggtaccact
     5221 tcatcacctc cgccgcgcgc tcgagcggcc acagcgtcgg gcccgccacc acggtgctga
     5281 gcgaatgcag cctgaactcg aaggaggtca cgacgccgaa gttgccgccg ccgccacgca
     5341 ccgcccagaa caggtcttcg ttctcgttcg cacttgccgt gacgaagcgc ccgtctgcca
     5401 ggacgacatc cacggccagc agattgtcga tggtcagccc gtaacggcgc gacaggtggc
     5461 cgatgccgcc gcccagggtg agcccgccca cgccggtggt cgagatgatt ccggcgggcg
     5521 tcgccatgcc gaacgcgtgc gtggcgtggt ccacatcgcc ccatttgcac ccgccctcga
     5581 cccgcacggt gtgcgccttg ggatcgaccc ggatgccgcg cagcgcggac aggtcgatca
     5641 ccatgccgtc ctcgcatacg cctaagcccc cgccgttgtg gccgccgccg cggacggcca
     5701 gcagcatctt gctctc
//
LOCUS       DASWIY010000029         9492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2239710, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000029 DASWIY010000000
VERSION     DASWIY010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9492
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2239710"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VGA59_01090"
     CDS             <1..222
                     /locus_tag="VGA59_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX9718301.1"
                     /translation="NRVIGQLRSALGKGFPIIGVGGVMSAQDAVSKIRAGADAVQIYT
                     GLIYRGPQLVSDAARALKALAAQPRPQNA"
     gene            complement(210..2321)
                     /locus_tag="VGA59_01095"
     CDS             complement(210..2321)
                     /locus_tag="VGA59_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9718302.1"
                     /translation="MRAAALFALALAAAGAAGAQTLPQVTVSATRSEAAPFDVPASVD
                     VIDGARLRAAGRPEMNLSESLAIVPGVTARDRQNHAQDLQLSIRGFGARSTFGVRGVR
                     LYVDGIPATMPDGQGQLSHIDLASAGRVEILRGPFSVLYGNSSGGVLQVFTEPGQGAP
                     TVAPSLAVGSDGLVRPGLRISGSTPALGYVLSANRFSTDGWREHSDAARGLANARLDI
                     QGAAGSEWTVVANAVALRADDPLGLTRAQFESAPRSADAAATSFDTRKTVRQSQLGLI
                     HEKRLAGGDRLRLLVYGGQRDTEQFQAIPTAPQASALHPGGVIGLERRYAGTDLRLTT
                     QTSLAQGPLEVVAGLSYDLMREQRRGWHNFSGATLGVLGALRRDEENRVANLDPYLQA
                     VWKFAPRWSLTAGVRRSTVRFSSSDRYVAGINGNDSGEVRYGATLPVVGLMVAWSPRL
                     RVYAALGRGFETPTFNELAYRPDGTAGLNFVLKPSRSDNLELGIKGRAPAGSRLRSEW
                     SAALFQTATAGEIVTQTNSGGRSTFQNAGATRRRGLELAWSAALAPAWQFQLSQTWLD
                     ARYRDAFAACSGTPCTVPTQSIPAGNRIPGTARSVSAAEIAWQPARGWRAGAELRRSS
                     RVYVNDTNSEAAPSFTVVALHAGYVFDFRGWALSASARVDNALDRRYAGSVIVNEGNG
                     RFFEPAPGRAYVLKLAGNYAF"
     gene            complement(2318..3445)
                     /locus_tag="VGA59_01100"
     CDS             complement(2318..3445)
                     /locus_tag="VGA59_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9718303.1"
                     /translation="MMDQVDAIVIGAGVVGLAVARALAQSGRETIVAEAQTAIGQGVS
                     SRNSEVIHAGLYYPPGSLKARLCVRGKQLLYELCATHGVNHRHCGKLVVANTDAEAAA
                     LRALQDRAVANGVPVQWLAAHEAQALEPGLRCIAALLSPTTGIVDSHGLMLALQGDLE
                     NAGGMVAVGSAVDGAVLGQGGQPHLIRMVDGTEVASRVLVNSASLHACALARRFAGLD
                     PQFVPREYFAKGNYFALPGKAPFSRLIYPAPDDAWLGVHLTLDLGGQAKFGPDLQWLD
                     VARAEDIDYKVDPARADGFYAEVRRYWPGLADGALQPSYSGVRPRIHGPAERAPDFRI
                     DGPRLHGVAGLVNLFGIESPGLTSALAIAEYLAALLRDMPP"
     gene            complement(3442..4293)
                     /locus_tag="VGA59_01105"
     CDS             complement(3442..4293)
                     /locus_tag="VGA59_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX9718304.1"
                     /translation="MRFPVHAVAGSTPLVLDSPHSGVNYPDDFGHACDPQALRQAEDT
                     HVEKLYDFAPALGAAWIEALFPRSYIDVNRNTTEVDLALLDGAWPDPVETDPKALAKV
                     RLGKGLVWRITDDGLPIYERRLSVRELRSRIERCWQPYHDAVRDAVAAAHARHGYSIH
                     LNCHSMPSVASAYSTEFPGLVHADFVIGDREGTTAAPELSRRICGFLESLGYGVAYNH
                     PYKGVELVRRYGDPGHHRHSVQVEINRKLYMDERTLELHEGFEPLKRHLRMLVEMLLR
                     NDPRQEL"
     gene            4476..4682
                     /locus_tag="VGA59_01110"
     CDS             4476..4682
                     /locus_tag="VGA59_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HEX9718305.1"
                     /translation="MNDEAFNLSIRKFLKMVGVNSQRDIEHAAAKAIAEGAIAGTGSF
                     PAKVRLEIPGLKLDVQFDGEIRLE"
     gene            4808..5647
                     /locus_tag="VGA59_01115"
     CDS             4808..5647
                     /locus_tag="VGA59_01115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9718306.1"
                     /translation="MPHDKLIEDRIGAVWAAPDATVLRRLREAQFVAHNIPLTQTICT
                     RPGQPLISESPRTVAIHESLERFVPGGPRGKTAVDLGCLEGGLSFELWRAGLTVLGVE
                     GRKENWERCLLVRDHFRAAGEMEFLLADVREYKPGRSFDVVVCSGLLYHLDDPAAYIA
                     ALGELTAEQGMLYLDTHVAPEDMDMAECEFRDFLSPLRSKAASGIQIRYREYAEDVKL
                     PESSVGNPVSLWMDGASHLELLYRAGFARVFELNGYFGAGETALKRRYARRYFVALKN
                     PAP"
     gene            complement(5692..5766)
                     /locus_tag="VGA59_01120"
     tRNA            complement(5692..5766)
                     /locus_tag="VGA59_01120"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5732..5734),aa:Val,seq:cac)
     gene            complement(5795..6511)
                     /gene="dnaQ"
                     /locus_tag="VGA59_01125"
     CDS             complement(5795..6511)
                     /gene="dnaQ"
                     /locus_tag="VGA59_01125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX9718307.1"
                     /translation="MRQIVLDTETTGLSAEGGDRIIEIGCVELLARKLSGNNRHFYLN
                     PGRDSHEDALKVHGISNEFLRDKPRFAGVADELLEYLAGAEIIIHNAAFDVGFLDMEL
                     ALLGKPAFTTCVGQVTDTLAMAKEMYPGKRNSLDALCDRLGVDNSGRTLHGALLDAEL
                     LADVYINLTRGQDALLIDIAAGDTVGGIAVRVDLTAFTLPVLLASEQESAAHEEVLKL
                     LDKASGGKTLWRIEQPGRMA"
     gene            6558..7313
                     /locus_tag="VGA59_01130"
     CDS             6558..7313
                     /locus_tag="VGA59_01130"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9718308.1"
                     /translation="MFDLAGKVAVVTGGNGGIGLGMARGLAQAGATVVLAGRDAAKSA
                     AAVQALAAEGHKADAIEADVTGEGDVARLFQALMLRHGRLDILVNNAGTNIRKAAHDM
                     SLDEWRNVMDTNLTSAFLCCRAARALMQEAGGGKIINIGSMLSIFGAPYAPAYGASKG
                     GVVQFTRSIATAWAADNIQANAVLPGWIDTELTRKAREQVPGLNERVLARTPAGRWGQ
                     PADLAGIAVFLASSASDFITGTAIPVDGGYSIS"
     gene            complement(7355..7693)
                     /gene="fdx"
                     /locus_tag="VGA59_01135"
     CDS             complement(7355..7693)
                     /gene="fdx"
                     /locus_tag="VGA59_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEX9718309.1"
                     /translation="MPTIKILPHPEYCPSGAEVTAPAGTSICEALLDNDIDIEHACDM
                     SCACTTCHVIVREGFNSLNEMDENEEDLLDRAWGLEPNSRLSCQAILAQKDLTVEIPK
                     YSINQAKEKH"
     gene            complement(7783..>9492)
                     /gene="hscA"
                     /locus_tag="VGA59_01140"
     CDS             complement(7783..>9492)
                     /gene="hscA"
                     /locus_tag="VGA59_01140"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEX9718310.1"
                     /translation="ILPSVVRYLENGGRQIGYDAQAAQAEDPENTIASVKRFMGRGLS
                     DIVNREKMPYRFVDKPGMVALQTRAGEKSPVEISAEILVTLRYRAEDTFNDDLYGAVI
                     TVPAYFDEAQRQATKDAAKLAGINVLRLINEPTAAAIAYGLDNASEGIYAVYDLGGGT
                     FDISILRLTKGVFEVIATGGDSALGGDDYDRALAEWMLAKAGVEATGASAKAAIKVQA
                     RAAKEQLSTSEVVTARIGLADVAGPEISVRRSNFEAVTADLTARTMVAVRKALRDAKL
                     ARDEVKGVVLVGGSTRMPQIRNAVAEFFGREPLTNLNPDEVVAIGAAIQANQLAGNNS
                     AGDLLLLDVIPLSLGIETMGGLVERIVPRNETIPTAKAQDFTTYQDGQTALALHVVQG
                     ERDLVQDCRSLARFELRGIPPMVAGAARIRVTFTVDADGLLSVGAKEQVSGVEARIDV
                     KPSYGLSDEQIARMLQESFTTAEQDMKARALAEAGVDAQRMLLATQSALDADGALLAP
                     EERAHIDSLMEQLRQVAAASHDAGAIEAATQALAKGTEAFAAERMNHSIQKALSGKNV
                     ETI"
BASE COUNT         1538 a         3192 c         3188 g         1574 t
ORIGIN      
        1 aaccgcgtga tcggccagct gcgttcggcg ctgggcaagg gattcccgat catcggtgtc
       61 ggaggcgtga tgagcgcgca agacgccgtc agcaagatcc gcgcgggtgc cgatgcggtc
      121 cagatctata ccgggctgat ctaccgcgga ccgcagctcg tgagcgacgc ggcgcgcgcg
      181 ctcaaggcgc tggcggcgca gcccaggcct cagaacgcgt agttgcccgc cagcttgagc
      241 acgtaagcgc gtcccggcgc cggttcgaaa aaccggccgt tcccctcgtt gacgatgacc
      301 gacccggcat agcgccggtc cagcgcgttg tcgacgcgcg cgctcgccga caaggcccag
      361 ccccggaagt cgaagacgta gcccgcatgc agcgccacca ccgtgaacga tggcgcggct
      421 tcgctgttgg tgtcgttgac gtaaacccgg ctggagcgcc gcagctcggc gccggcgcgc
      481 cagccgcgcg ccggctgcca cgcgatttcg gcggccgaca ccgatcgtgc ggtacccggg
      541 atgcggttgc ccgccggtat cgactgcgtg ggcaccgtgc aaggcgtgcc gctgcaggcg
      601 gcgaacgcgt cccggtagcg ggcatccagc caggtctgcg acagctggaa ctgccaggcc
      661 ggcgccagcg ccgcgctcca ggccagttcg aggccgcggc gccgggtggc gccggcattc
      721 tggaaggtac tgcggccgcc gctgttggtc tgcgtcacga tttcgcccgc ggtcgcggtc
      781 tggaacagcg ccgcgctcca ttcgctgcgc aggcggctgc ccgccggcgc gcggcccttg
      841 atgccgagtt ccaggttgtc gctgcgcgac ggctttaaca cgaagttcag gccggcggtg
      901 ccgtcgggcc ggtaagccag ttcgttgaag gtgggtgttt cgaagccgcg gcccagcgcc
      961 gcatacacgc gcagccgggg tgaccaggca accatcagtc ccacgaccgg cagcgtcgcg
     1021 ccatagcgga cttcgccgct gtcgttgcca ttgatgccgg cgacatagcg gtcgctcgac
     1081 gagaagcgca ccgtgctgcg ccgcacgccg gccgtcaggc tccagcgcgg cgcgaatttc
     1141 cacacggcct gcaggtacgg atcgaggttg gcgacccggt tctcctcgtc acgccgcaac
     1201 gcgcccagca cgcccagcgt ggcgccggag aagttgtgcc agccacggcg ctgctcccgc
     1261 atcaagtcgt aggacaggcc cgccacgact tcgagcggcc cttgcgccaa cgatgtctgc
     1321 gtggtcaagc gcaggtcggt gccggcatag cggcgctcca ggccgatcac gccaccgggg
     1381 tgcagcgcgc tggcctgggg ggcggtgggt atcgcctgga actgttcagt atcgcgctgg
     1441 ccgccgtaga ccagcaggcg caggcgatcg cctcccgcaa gccgcttctc atggatcaat
     1501 cccagctggc tttgcctgac ggtcttgcgc gtgtcgaacg acgtggccgc cgcatcggcg
     1561 ctgcgcggcg cggattcgaa ctgggcccgg gtgaggccga gcggatcgtc cgcccgcagc
     1621 gccacggcgt tcgccacgac ggtccattcg ctgcccgcgg cgccctggat gtccagccgt
     1681 gcattggcca ggccgcgcgc ggcgtcgctg tgctcgcgcc agccatcggt ggagaagcga
     1741 ttggcgctga gcacatagcc cagagccggg gtcgaaccgc tgatgcgcag gccgggccgc
     1801 accagaccgt cgctgccgac ggcgaggctg ggcgcgacgg tgggcgcgcc ctggcccggt
     1861 tcggtgaaga cctgcagcac cccgcccgac gagttgccgt acagcacgga gaaagggccg
     1921 cgcagaattt cgacgcgacc ggccgacgcc agatcgatat gggacagctg cccctggcca
     1981 tccggcatcg tggccggaat gccgtcgaca tagaggcgca ccccgcgcac gccgaatgtc
     2041 gagcgcgcgc caaagccgcg gatggacagc tggaggtcct gggcatggtt ctggcggtcg
     2101 cgcgcggtga cgccgggaac aatcgccagg ctttccgaca ggttcatctc gggccggccg
     2161 gcggcccgca gtcgcgcgcc gtcgatcacg tccaccgatg ccggcacgtc gaacggcgcg
     2221 gcttcgctgc gggttgcgct caccgtgact tgcggcagcg tctgcgcccc ggcagcgccc
     2281 gccgcggcca gtgccagcgc gaacagcgcc gccgccctca tggcggcatg tcccgcagca
     2341 gcgccgccaa atattcggca atcgccaagg cgctggtgag ccccggcgat tcgatcccga
     2401 acaggttgac caggcccgcc acgccatgca accgcggccc gtcgatgcgg aaatccggtg
     2461 cgcgctcggc cggcccgtga atcctgggcc gcacgccgct gtagctgggc tgcaaggccc
     2521 cgtccgcgag gccgggccag taccgccgca cctcggcata gaacccatcg gcgcgggcgg
     2581 gatcgacctt gtaatcgata tcctcggctc gcgcgacatc gagccattgc agatcgggcc
     2641 cgaacttcgc ctgcccgccc aggtccagcg tgaggtgcac gcccagccat gcatcgtccg
     2701 gcgccggata gatcaggcgg gaaaacggcg ccttgccggg cagcgcaaag tagttgcctt
     2761 tggcgaaata ttcgcgcggc acgaattgcg gatccagccc cgcaaaacgc cgcgccagag
     2821 cgcaagcatg cagcgacgcc gaattgacga gaacccggga tgccacttcg gtgccgtcca
     2881 ccatcctgat caggtgcggc tggccgccct gccccagcac tgcgccatcc acagccgaac
     2941 ccacggccac catgccgccg gcgttttcca ggtcgccttg cagcgccagc atcaatccat
     3001 ggctgtccac gattcccgtc gtgggtgaca acaaggcggc aatgcagcgc aagcccggtt
     3061 ccagcgcttg cgcctcatgc gctgccagcc actggactgg aacgccgttc gcgacggcgc
     3121 gatcctgcag tgcccgcagg gccgccgcct cggcatcggt gttggcgacg acgagcttgc
     3181 cgcaatggcg atgattgacg ccgtgcgtag cgcacagctc atagagcagt tgcttgccgc
     3241 gcacgcacag ccgtgccttg agcgaccccg gcggatagta gaggccggca tgaatgactt
     3301 cgctgttgcg cgaacttacg ccctgcccga tggccgtctg cgcttcggcc acgatggtct
     3361 cgcggccgct ttgcgccagt gcccgcgcaa cggccagccc gacgacgccg gccccgatga
     3421 cgatcgcgtc gacctggtcc atcacagttc ctggcgagga tcgttgcgca gcagcatctc
     3481 gaccaacatc cgcaggtggc gcttgagcgg ctcgaagcct tcatgaagct ccagcgtgcg
     3541 ctcatccatg tagagcttgc ggttgatctc cacctgcacg ctgtgcctgt gatgtccggg
     3601 atcgccgtag cggcgcacca gctccacgcc tttgtagggg tggttgtagg cgacgccgta
     3661 gcccagggat tcgaggaagc cgcagatcct gcgcgacagt tcgggggcag ccgtcgtgcc
     3721 ctcccggtcg ccgatcacga aatcggcgtg gacgagaccg gggaactcgg tcgaataggc
     3781 ggaagcgacg gacggcatcg aatggcaatt caggtggatg ctgtagccgt gccgggcatg
     3841 ggccgcggct acggcgtcac gcacggcatc atggtagggc tgccagcaac gctcgatgcg
     3901 ggaacgcagc tcccgcaccg acagccgccg ctcatagatc ggcaggccat cgtccgtgat
     3961 gcgccagacc aggcccttgc ccaaccgcac cttggccaat gccttggggt cggtctccac
     4021 cggatcgggc caggcgccgt cgagcaaggc gaggtcgacc tcggtggtgt tgcggttcac
     4081 gtcgatgtag ctgcgcggga acagcgcctc gatccaggcc gcacccaggg ccggggcaaa
     4141 gtcgtagagc ttttccacat gggtgtcctc ggcctggcgc agggcttgcg gatcgcaggc
     4201 atggccgaag tcgtccgggt aattcacgcc gctgtgcggt gaatcgagca cgagcggtgt
     4261 gctgcccgcg acggcatgga cgggaaaacg catggggcat tgtggcgcag cggcgagcgg
     4321 ccgggagcgc ccaactgccg ggcaaagcca ggcgtgaccg cgcccggtgt ggcgcgcccc
     4381 ccaacggatg atgggaagcg gatgataggg gtgccggtag cgccgttggg cgccgcaacc
     4441 ggcgcattcc gattaattgc gattggagat catcgatgaa cgacgaagca ttcaacctca
     4501 gcattcgaaa attcctgaaa atggttggcg tcaattccca gcgcgacatc gagcacgcgg
     4561 cggcgaaggc catcgccgag ggtgccatcg ccggcaccgg gagctttccc gccaaggtga
     4621 ggctggaaat cccgggtctg aagctggacg tccagttcga cggcgagatc aggctcgaat
     4681 gagatttgcg tcctgttcgg gaatcccgtg cgccccctag aatgtgcggg ttcgccgcga
     4741 ggtgggtcgg cggcgccgcc aagccaccgg aaagcggcct ctctgttact cacgatcgaa
     4801 gcggcccatg ccccacgaca aattgatcga agaccggatt ggcgccgtct gggccgcccc
     4861 tgatgcgact gtcctgcggc gcctgcgcga ggcgcaattc gtggcgcaca acattccgct
     4921 gacgcaaacg atttgcacgc gccccggcca acccctgatc tccgagtcgc cgcgcacggt
     4981 ggctatccac gagtcactgg agcgctttgt cccgggcggc ccgcgcggaa agaccgcggt
     5041 ggacctgggc tgtctggaag gcggcctgtc attcgaactg tggcgcgcgg ggctgactgt
     5101 gctcggcgtg gagggccgca aggaaaactg ggagcgctgc ctgctcgtgc gcgaccattt
     5161 ccgcgccgcg ggcgaaatgg aattcctgct ggctgacgtg cgcgagtaca agcccggcag
     5221 aagcttcgac gtggtcgtct gctccggcct gctctaccat ctggacgatc ccgcagccta
     5281 catcgccgcc ctgggcgagc tcaccgccga gcaaggcatg ctgtacctgg acacccatgt
     5341 cgcgccggaa gacatggaca tggccgaatg cgagttccgt gattttcttt cgccgctgcg
     5401 cagcaaggcc gcctcgggaa ttcagatccg ctatcgcgag tacgcggaag acgtgaagct
     5461 gcccgagtct tcggtcggca acccggtttc gctttggatg gatggtgcct cgcacctgga
     5521 gctgctgtac cgcgcgggct ttgcccgcgt attcgaattg aacggctact tcggcgcggg
     5581 cgaaacggcg ctcaagcgcc gatacgcgcg ccgctacttc gtggcgctga agaacccggc
     5641 gccttgagga aagacggccc cgatccgagg actcgtgcga aagcgtcgac ttggtgggcg
     5701 gtgcagggat tgaacctgcg acccctcccg tgtgagggga gtgctctacc gctgagctaa
     5761 ccgcccgttt ttcccattgc gggaaagcct cgaattatgc catacggccg ggctgctcga
     5821 tccgccacag ggtcttgccg ccgctggcct tatcgagcag tttcaggact tcttcatgcg
     5881 cggccgattc ctgctcgctg gccagcagca cgggcaaggt gaacgccgtg agatcgaccc
     5941 gcaccgcgat gccgccgacc gtgtcgccgg cggcgatgtc gatcagcagc gcgtcctggc
     6001 cgcgcgtgag gttgatgtac acgtcggcca gcaactcggc atcgagcaag gcaccgtgca
     6061 gggttcggcc ggaattgtcc acgcccaggc ggtcgcacag cgcgtcaagg ctgttgcgct
     6121 tgcccgggta catctccttg gccatggcca gcgtatcggt cacctggccc acgcaggtcg
     6181 tgaacgctgg cttgcccagc agcgccagtt ccatgtcgag gaagccgaca tcgaacgccg
     6241 cgttgtggat gatgatttcg gcgccggcaa ggtactccag cagctcgtcg gccacgcccg
     6301 cgaaccgggg cttgtcgcgc aggaactcgt tgctgatgcc gtgcaccttg agcgcgtctt
     6361 catggctgtc gcgcccgggg ttcaggtaga aatgcctgtt gttgccgctc agcttgcgcg
     6421 cgaggagttc cacgcagccg atctcgatga tgcggtcgcc gccttcagcg gaaaggcccg
     6481 tggtttcggt gtcaaggacg atctggcgca tggccggcat tatgcttgag cgtcacagca
     6541 gtcaaggagc ccgcatcatg ttcgacctcg ccggaaaagt tgccgtcgtc acgggaggca
     6601 atgggggaat cggccttggc atggcgcggg ggctggcgca ggccggggcc accgtggtgc
     6661 tcgccggccg cgacgccgcc aaatcggcgg ccgcggtaca ggccctggcg gccgaaggcc
     6721 acaaggcgga tgcgatcgag gccgacgtga ccggcgaggg cgacgttgcg cggttgttcc
     6781 aggcgctcat gctgcgccat ggccggctcg acatcctggt caacaacgcg ggaaccaaca
     6841 tccggaaagc cgcgcacgac atgagcctgg acgaatggcg caacgtcatg gacaccaacc
     6901 tgaccagcgc cttcctgtgc tgccgcgccg cgcgcgcgct gatgcaggaa gccggcggcg
     6961 gcaagatcat caacatcggc tccatgctgt cgatcttcgg ggcaccctac gcgcccgcct
     7021 acggcgccag caagggcggg gtcgtccagt tcacgcgctc gattgccacc gcttgggccg
     7081 cggacaacat ccaggcgaac gcggtgctac ccggatggat agacaccgag ctcacgcgca
     7141 aggcgcgcga acaggtcccg ggattgaacg agagggtgct ggcgcgcacg ccggcgggtc
     7201 gatggggaca gccggccgac ctggcaggca ttgccgtttt cctggcgagc agcgcctcgg
     7261 acttcatcac cggaaccgcg atcccggtcg atgggggcta ttcgatctcg taggccggca
     7321 acgagcgccc gctcttcacg caggccacct gcgcctaatg cttttccttg gcctggttga
     7381 tcgagtactt cgggatttca acggtcaagt ccttctgcgc caggatcgcc tggcaggaaa
     7441 gccgcgagtt gggctccagg ccccaggcgc gatcgagcag gtcctcctcg ttctcgtcca
     7501 tttcgttgag cgagttgaag ccttcgcgca cgatcacgtg gcaggtcgtg caggcgcagc
     7561 tcatgtcgca ggcgtgctcg atgtcgatgt cgttgtccag cagcgcctcg caaatcgagg
     7621 tgccggcggg cgccgtcact tcggcgccgc tggggcagta ctcggggtgc ggcaggatct
     7681 tgatcgtggg catttttcag tcagttgggg acagagctaa agacctgtcc cgcaattctc
     7741 agtcagttgg ggacagagct gaagatctgt cccacaagtt aatcatattg tctcgacgtt
     7801 cttgccggat agcgccttct ggatgctgtg gttcatgcgc tcggcggcaa aggcttccgt
     7861 gcccttggcc agcgcctgcg ttgccgcttc gatcgcaccg gcgtcatggc tggcggcagc
     7921 cacctggcgc aactgctcca tgagcgaatc gatgtgcgcc cgctcctcgg gcgccaggag
     7981 ggcgccgtcc gcatcgagcg cgctttgcgt agccagcaac atccgctggg catccacgcc
     8041 ggcctcggcc agcgcccgcg ccttcatgtc ctgctccgcg gtggtgaagc tctcctgcag
     8101 catcctggcg atctgctcgt ccgacaagcc ataggatggc ttgacgtcga tgcgcgcttc
     8161 gacgccgctg acctgctcct tcgcgccgac actgagcagc ccatcggcat cgaccgtgaa
     8221 cgtcacacgg atgcgcgcgg ccccggcaac catcggcgga ataccgcgca gctcgaaccg
     8281 cgccaggctg cggcagtcct gcaccaggtc gcgctcgccc tggaccacgt gcagggccag
     8341 cgcggtctgg ccgtcctggt aggtggtgaa gtcctgggcc ttcgccgtcg gaatggtttc
     8401 attgcgcggc acgatccgct cgaccaggcc gcccatggtc tcgatgccca gcgacagcgg
     8461 gatcacgtcc agcagcagca ggtcgcccgc cgaattgttg ccggcgagct ggttggcctg
     8521 gatggcggcg ccgatggcaa ccacttcgtc ggggttcagg ttggtgagcg gctcgcgccc
     8581 gaagaactcc gcgaccgcgt tgcggatctg cggcatgcgc gtcgacccgc cgaccagcac
     8641 cacgcccttc acttcgtccc gggccagctt cgcgtcgcgc aaggccttgc gcacggccac
     8701 catggtgcgg gcggtcagat cggcggtaac cgcctcgaaa ttgctgcgcc ggaccgaaat
     8761 ttcggggccg gcgacatcgg ccagcccgat ccgcgccgtg acgacctccg acgtggacag
     8821 ctgctccttg gcggcacgcg cctgcacctt gatcgctgcc ttggcgctcg cccccgtcgc
     8881 ctccacgccg gctttggcca gcatccattc ggccaacgcg cggtcgtagt cgtcgccgcc
     8941 cagggccgaa tcgccgccgg tcgcgatcac ctcgaacacg cccttggtca gccgcaggat
     9001 cgagatgtcg aaagtgccac cgcccaggtc atagaccgca tagatgcctt cgctggcatt
     9061 gtccaggccg taggcgatgg cggcggccgt gggctcgttg atcaaccgca gcacattgat
     9121 gccggccagt ttcgccgcat ccttggtggc ctggcgttgc gcctcgtcga aatacgcggg
     9181 aaccgtgatc acggcgccgt agaggtcgtc gttgaaggtg tcctcggcgc ggtaacgcag
     9241 ggtcaccagg atttcagcgc tgatttccac cggggacttt tccccggcgc gggtttgcag
     9301 cgccaccatg cccggcttgt ccacgaaccg atagggcatc ttttcgcggt tgacgatgtc
     9361 ggaaagaccc cggcccatga aacgcttgac cgaggcgatg gtgttctccg gatcctcggc
     9421 ctgcgcggcc tgggcgtcgt agccgatctg gcgcccgccg ttttccaggt agcgcaccac
     9481 cgagggcagg at
//
LOCUS       DASWIY010000030         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_392932, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000030 DASWIY010000000
VERSION     DASWIY010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392932"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..396
                     /locus_tag="VGA59_01145"
     CDS             103..396
                     /locus_tag="VGA59_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718311.1"
                     /translation="MANRKPPRYVPTLTEVVGTAGESKLAESAGISQEQVIQRVMQRI
                     DLTLERRLREAIAATVLEQTRSLAPMLREEIESVVRETVAQAFEQELAAGRQP"
     gene            566..1696
                     /locus_tag="VGA59_01150"
     CDS             566..1696
                     /locus_tag="VGA59_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9718312.1"
                     /translation="MQMNLKLTVAAAIAALASVAGAQDVQVVKIGHVGPVSGPQASYG
                     RDNENGARMAIEDLNAQNIVINKKKIKFELLAEDDAADPKQGTAAAQKLCDSKVAGVV
                     GHLNSGTTIPAAKVYNDCGIPMITPSATNPALTKPGYKTTFRLLANDLSLGAGLALYA
                     ADTLKLKTVAVIDDRTAYGQGVADIFKKTVQKKGMKVVDTQFTTDKAVDFMAILTSIK
                     GKNPDAIFFGGMDVQAGPMLRQMEQLGLTKQKYFGGDGICTQDVIKQSGGAKTLENVI
                     CAEGGASLEKMPGGVAWKKRYDAKYPGQFQVYAPYVYDAFFVMVDAMKRANSWDPKVY
                     TPFIGKANFKGITTTVQFEPNGELKNPAITLYTYKDGKKVAL"
     gene            1953..>3387
                     /locus_tag="VGA59_01155"
     CDS             1953..>3387
                     /locus_tag="VGA59_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX9718313.1"
                     /translation="MEVLAKLPAVDRRDNGELKQVLKALGAFRRGEVGLTLPTEWDGI
                     YGKIAAEFNELTSQAARTSQKLKAIDGGARPGLKQRRLADEKLTGFWQKDVAVVNSVL
                     EEVDVLSEHTRAFLGALNDLKKGNFVAQLPQDWTGVFGKVADAFNDVVGENLRMSHEL
                     ARLSRVVGKEGKLKERAFVPNASGFWRDSAESINSLIADLVHPTSEVARVIGAVAQGD
                     LSKSMALEADGRPLEGEFLRTAMIINKMVQQLGTFSAEVTRVAREVGTEGKLGGQADV
                     QGVAGTWKDLTDSVNFMASNLTAQVRNIAGVTKAVATGDLSKKITVDVKGEILELKNT
                     INTMVDQLRSFASEVTRVAREVGTEGKLAGRADVQGVAGTWKDLTESVNSMGSNLTAQ
                     VRNIAEVTKAVAAGDLSKKITVDVKGEILELKNTINSMVDQLRSFASEVTRVAREVGT
                     EGKLGGQADVQGVAGTWKDLTDSVNSMG"
BASE COUNT          700 a          984 c         1114 g          589 t
ORIGIN      
        1 gcaggaagac gatcgccagc agggacgccg gcggtggaag cattacgccg accgcggcta
       61 cgccatcaag ggcgaggacc ttggcgcgaa ggagaccggc tgatggccaa tcgcaagccg
      121 ccgcgctatg tgccgaccct gacggaagtg gtcgggactg ccggtgaatc caagcttgcc
      181 gaaagcgccg gcatttcgca ggagcaggtc atccaacggg tcatgcagcg gatcgacttg
      241 acgctcgagc gccgtctgcg ggaggcgatc gcggccacgg tgctcgagca gacccgctcg
      301 cttgcgccca tgctgcgcga ggaaatcgaa tcggtggtgc gggaaacggt cgcccaggcg
      361 ttcgagcagg aactggccgc gggccggcag ccgtgatgtc ggctgggtaa cggctctcca
      421 ttagccgccc tacgggccct gtaattgctc ttatctgggt ttacctgaca ttcccctatg
      481 gctcgggcct ggaaattgcg atatgttcgc ggccgttgag cctataaaaa ttctcaacct
      541 ttttccctac tctggaggta agtttatgca gatgaatttg aaattgacgg ttgcggccgc
      601 cattgccgct cttgcgagcg ttgcgggcgc gcaagacgtc caggtggtca agatcgggca
      661 tgtaggcccg gtctcgggtc cccaggcctc gtacggcagg gacaacgaga acggcgcgcg
      721 catggccatc gaggacctca acgcgcagaa tatcgttatc aacaagaaga agatcaagtt
      781 cgagctgctg gccgaagacg atgccgccga tccgaagcaa ggcaccgctg ccgcgcagaa
      841 actgtgtgac tccaaagtcg caggcgttgt gggccacctg aattcgggca ccaccatacc
      901 cgccgccaag gtctacaacg attgcggcat cccgatgatc accccgtcgg ccacgaaccc
      961 cgccctcacg aagccgggtt ataagactac cttccggctc ttggccaatg acctgtcgct
     1021 gggcgccggc ttggccctct atgccgcgga taccctgaag ctcaagacag tggccgtcat
     1081 cgacgaccgc acggcttatg gccaaggcgt tgccgacatc ttcaagaaaa cggtccaaaa
     1141 gaagggcatg aaggttgtgg atacacagtt cacgaccgac aaggccgttg acttcatggc
     1201 catcctcacc tccatcaagg gcaagaatcc cgatgccatc ttctttggcg gcatggacgt
     1261 gcaagccggc ccgatgctgc gccagatgga acagctcggc ctgaccaagc agaagtactt
     1321 cggcggcgac ggcatctgca cgcaggacgt gatcaagcag tccggtggcg ccaaaaccct
     1381 ggagaacgtg atctgcgccg agggcggcgc ttcgctcgag aagatgcccg gtggcgtggc
     1441 ctggaagaag cgttacgacg ccaagtaccc cggccagttc caagtgtacg caccgtatgt
     1501 ttacgacgcg tttttcgtga tggtcgacgc catgaagcgc gccaacagct gggacccgaa
     1561 ggtctatacg cccttcatcg gcaaggccaa cttcaagggc atcaccacca ccgtgcagtt
     1621 cgagcccaat ggtgaactga agaatcccgc catcaccctg tacacctaca aggacggcaa
     1681 gaaggtcgcg ctctagtaac tcagcgattg ccgccaatga gaagccgccg cgaggcggct
     1741 tttctcgttg gggagcgccg cggcttttcg ccaacgccac aatgtttgtc gtgttttgcc
     1801 gcttgttctt gtaggcgctt tcctacagcc ggtgtgccat agtgtttgca acgtgcggtt
     1861 tatgcggcgc ccggttgtcg ctcgccccgg aacacagtac gctgcttcga tttgttgctc
     1921 gacggacggg atgcttacgt atggggaaaa gcatggaagt actggcaaaa ctgcctgcag
     1981 tggatcggcg cgataacggc gagctgaaac aggtcttgaa ggcactgggc gcattccggc
     2041 ggggtgaagt cggcctcacg ctgccgaccg agtgggatgg catctacggc aagatcgccg
     2101 cggaattcaa cgaactcacg agccaggcgg cgcggacgtc gcagaaactc aaggccatcg
     2161 acggcggcgc gcgcccgggg ctcaagcaaa ggcggctggc cgacgagaag ctcactggct
     2221 tctggcagaa agacgtggcc gtggtcaatt ccgtgctcga agaagtggat gtattgtcgg
     2281 agcacactcg cgccttcctc ggagcgctga acgacctgaa gaagggcaat ttcgtagccc
     2341 agctgcccca ggactggacc ggcgtgttcg gcaaggtggc ggacgctttc aacgatgtcg
     2401 tgggcgagaa cctgcgcatg tcgcatgagc tggcgcggct gtcccgcgtt gtgggcaagg
     2461 agggcaagct caaggagcgc gcgttcgttc ccaacgccag cggtttctgg cgcgattccg
     2521 ccgaatccat caactcattg atcgccgacc tggtgcatcc gacgtccgaa gtggcgcggg
     2581 tgatcggcgc ggtggcccag ggcgacctgt ccaagagcat ggcgctggag gcggatggcc
     2641 ggccgctgga aggcgagttc ctgcgcaccg ccatgatcat caacaagatg gttcagcagc
     2701 tgggcacctt ctccgccgag gtgacgcgtg tggcgcggga agtgggtacc gaaggcaagc
     2761 tgggcggtca ggccgacgtg cagggcgtgg ccggcacctg gaaggatttg acggactcgg
     2821 tgaacttcat ggcatccaac ctcaccgccc aggtgcgcaa catcgcgggc gtgacgaagg
     2881 cggtggcgac gggtgacctg tccaagaaga tcacggtgga cgtgaagggc gaaatcctgg
     2941 agctgaagaa caccatcaac accatggtgg accagctgcg gtcgttcgct tccgaagtga
     3001 cgcgggtggc gcgggaagtg ggtaccgaag gcaagctggc cggccgcgca gacgtgcagg
     3061 gcgtggccgg cacctggaag gacttgaccg agtccgtgaa ctccatgggc tcgaacctca
     3121 cggcccaggt gcgcaacatc gcggaggtga ccaaggcggt ggcggccggt gacctgtcca
     3181 agaagatcac cgtggacgtg aagggcgaaa tcctggagct gaagaacacc atcaacagca
     3241 tggtggacca gttgcgctcg tttgcatcgg aagtgacgcg ggtggcgcgg gaagtgggta
     3301 ccgaaggcaa gctgggcggg caggccgacg tgcaaggcgt ggccggcacg tggaaggact
     3361 tgaccgactc ggtgaattcc atgggct
//
LOCUS       DASWIY010000031        14730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1496356, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000031 DASWIY010000000
VERSION     DASWIY010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 14730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14730
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496356"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..671)
                     /locus_tag="VGA59_01160"
     CDS             complement(156..671)
                     /locus_tag="VGA59_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9718314.1"
                     /translation="MPTKRSKPEEITPAGQLEEARLHYVLGYQLAQASIVTNLLFTQL
                     IGEPLALRRVEYTLLTLIAENPGGSHARLARALAVSSPNITTWIDKLEKRGFVKRRQS
                     ESDRRLQVLHATPKGLALAREATQRILKAETSALHALSPGERLILAELLHKVACARTE
                     LPNGVSTETTG"
     gene            931..2595
                     /locus_tag="VGA59_01165"
     CDS             931..2595
                     /locus_tag="VGA59_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEX9718315.1"
                     /translation="MVLTIAVTVSGCGDGGSSLPRLSAATPATFAGGCGDLPGKLSLA
                     NTTITTATSVPAGSLTVAGQAIPEHCLVTGRMFDRVSAVDGQAYAIGFEMRLPMQWNG
                     RFFHQGNGGIDGTVVTAVGAFGGGPLTNALLQRFAVLSSDAGHRAAQGPFFGIDPQAR
                     LDYGYQAVGKLTPMAKSLIQTAYGKGPDRSYLGGCSNGGRHAMVAASRYADQYDGILA
                     GAPGYNLPKAAIANIAGGQLYASVATNPADISTGFTTAERTLVANAVLSRCDALDGAT
                     DGLVQDVAACQSAFSLDRDVPTCTGARDGTCLSSAQKTAIGKIFSGPTTSSGALIYSS
                     FPYDGGLSGAGIPFWEFTAPLQLDSGAVGLIFKVPPESAEGFNGPAFALSANLDTLVA
                     QINATNSLYTESGMTFMTPPNPTNLSVLKNRGAKMLVYHGVSDPIFSVNDTETWYKGL
                     QSANGGDASEFARFFRVPGMGHCSGGPATDQFDVITPLVNWVEQGQEPAQVIATARGP
                     GNAAVANPEVPSSWAAGRTRPLCPYPKVARYAGSGSLEVASSFRCE"
     gene            2999..3955
                     /gene="dctP"
                     /locus_tag="VGA59_01170"
     CDS             2999..3955
                     /gene="dctP"
                     /locus_tag="VGA59_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9718316.1"
                     /translation="MTGAALAGASMMPAWAQAKPVLRFAAVFSDKDIRADMMRMLAKD
                     LEADFKVDLFLNSTLFKQGTELVALQRDNLELGNIGPQDFSKQIPAWSILTSAYLFRD
                     ANHLNAFFASDLGAQMKKMVEDQVKVKILGPTFFGTRQVGLKVKKKINTPADMAGIKL
                     RMPPGDAWQLLGRSLGANPTPMAYAETYTGLQTGAIDGQDNPLPNVQNMKFNEVMSQI
                     VLTSHLIGFDTLAINLKTWQAMSPAKQKSFQAAVDKALAWSTAEHLKREAELIAGFKA
                     QGLDVYTPNVNAFREYAQKVYLESPEAKTWPAGMLDKINAMK"
     gene            4025..4549
                     /locus_tag="VGA59_01175"
     CDS             4025..4549
                     /locus_tag="VGA59_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9718317.1"
                     /translation="MASKSLAGKIGAFSKRFAEVVAAGLMAAIFVSFIVQIVFRYVFN
                     FPVGWTSELSVAAWLWLVLWGAGFVLKEEEEIRFDLLSGGAGRRTRFVMAIVTGVAII
                     VLFAMSLPAAYSYVTFMKVESTSYLKIRFDWLFSIYLMFSVAVIVRYLWKLGQLLRGR
                     ISEPPEATKMSSAL"
     gene            4546..5847
                     /locus_tag="VGA59_01180"
     CDS             4546..5847
                     /locus_tag="VGA59_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9718318.1"
                     /translation="MNPASPFSLAVMALVALSLLGLPIGHAMIGGSILYLYLAGLDMG
                     TAAEQLLNGMYSSYLLLAIPLFIFAATIMSTGSILDRLLRFCNALVGRFPGGLAQVNV
                     VQSIVFASMSGSALADAAGSGKLMQALMTREGKYTKSFAAALTAVSAVIGPILPPSIP
                     LVLYALVSDASIGYLFIAGVFPGLLLAAVQLALIALMAKRRNFPVEAAVPLRELPGIA
                     REAFPALLMPVILLGFLYSGVTTPTEAAAVAAAYALLISTALYRSVSWPDLYQSLVSS
                     ARVTVSIGMLIAGALVFNYVITIENIPGTLSTLLKGFELSPLGFLLIANVVLLVLGCF
                     LEGSTIILVILPVLLPTAQALGVDPVHFGIVAVTNIMIGLVTPPYGLLLFMMVKIADV
                     PLKDLVIEVLPFLGVMFVALAVITLFPDFVLYLPRLVGYKG"
     gene            6007..6891
                     /locus_tag="VGA59_01185"
     CDS             6007..6891
                     /locus_tag="VGA59_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX9718319.1"
                     /translation="MANVIDREIHIDAINPLGLNGIEFIEYATSRPQAVGQALESMGF
                     RPIARHRSREVLLYRQGAMNVVINAHGNPSRGSLQLGDTPAIGAVAFRVQDAAMAYAR
                     ALDRGAWAVPTHVEVMELNIPAIHGVGSSRIYFVDRYREFSIWDVDFVPIPGVDQHPP
                     ALAGLHWFGLVQYIDYDRMADWCEFYHELFGFAELPDDERFGIMPNGKILASPCRTFF
                     LQLVEPPPGLDDVQCEEMLQRIGLGTPDVPAAVTALRGRGVEFVEGAHVHTDVRGALT
                     KPLAGMMFELVHDERAAS"
     gene            6888..7799
                     /locus_tag="VGA59_01190"
     CDS             6888..7799
                     /locus_tag="VGA59_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX9718320.1"
                     /translation="MTQRSGNFDDFGMATVTLAGPLEGKLKAIREAGFSQVMLEASDV
                     AGHPGGMDAALAAVRSSGLRVTGFQWLRDFEGLAGRQRDYKVEVAKAMLQMAHALGAP
                     MLLVASTTNSIAAQDLDAVARDLRKLAILAVPLGLKVAYEGMSWSRTVTDFVAAWDVV
                     CRADAPNLGLAIDSAHTLVSQTPLEELELIEPHRIFIVRLADFMEPQARSDEKSMMTR
                     TQLRVFPGEGLHSAAVADLVTRLAALGYSGDYSFEVFNADYQQLPPPLVAERARAASQ
                     WLGEDVLRRSVPLPSSMRLRQNSSPRD"
     gene            complement(7853..8293)
                     /locus_tag="VGA59_01195"
     CDS             complement(7853..8293)
                     /locus_tag="VGA59_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9718321.1"
                     /translation="MTERTVFQSMSQKHVLSLGPNASVWEAACVMTRASCGSVLVVDL
                     PGTLLGIVTERDLMTRVLAKALDPAKTKVCDVMTPNPLCVPPETLVSDAVVIMIERGF
                     RHLPVIGDGSKILGVFSVRDALPREIGNALSMAEFNEQVNDALG"
     gene            complement(8345..9400)
                     /locus_tag="VGA59_01200"
     CDS             complement(8345..9400)
                     /locus_tag="VGA59_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX9718322.1"
                     /translation="MTYIAKPRLRHPSLTANKVGYTRRDYEGRISTLCAGCGHDSISA
                     AIIEACWELDIEPHRVAKLSGIGCSSKTPDYFLGASHGFNTVHGRMPSVLTGASLANR
                     DLLYLGVSGDGDSASIGLGQFANAMRRGVCMAYIVENNGVYGLTKGQFSATADRGSKS
                     KKGVVNSDSPVDLVALALQLGATYVGRSFSGDKAQLVPLVKGAMAHGGAAFIDVISPC
                     VTFNNHGGSTKSYDYVRQHNEAVSRIDFITPRGEITAQYEPGAVIEVQQHDGSKLRLR
                     KLHQDYDPTDRYGAISYMQTHQARGEIVTGLLYVDPVATDLHMALNTSQKPLAALGAP
                     QLCPGSKALDAINAALR"
     gene            complement(9397..11247)
                     /locus_tag="VGA59_01205"
     CDS             complement(9397..11247)
                     /locus_tag="VGA59_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX9718323.1"
                     /translation="MKKIEAVNDFVIKFANVNGSGSASANELFAKAILRMGVPVSPRN
                     IFPSNIQGLPTWYEARVCEKGYAGRRGGVDMMVAMNPQTWDTDVAEIESGGYLFYDST
                     RPLPASRFREDVHVIGMPLTEITNAVYTDARQRQLFKNIIYVGALSVLLGIDADVFEK
                     LFAEQYKGKERLLDSNIRALHLGRDFVTEHLEYPLGIRVRRSDKVGDQIFVDGNSAAA
                     LGCVYGGATVAAWYPITPSSSVPEAFQKYCEKYRVDPGSGRYNYAIVQAEDELASIGM
                     VIGAGWNGARAFTATSGPGISLMTEFIGLAYFAEIPVTIIDVQRGGPSTGMPTRTQQS
                     DLIACAYASHGDTKHVLLFPQDPHECFEHAAAALDLADRLQTPVFLMTDLDIGMNQRL
                     AKPFVWDDARDYDRGKVMTAAELEAGKDFGRYKDVDGDGIPWRTLPGTHPTKGSFFTR
                     GTTRDPYARYSERGPDYTYNMERLLRKFKTAAQLVPQPVVRVAAQKTPLGVIYFGSTS
                     PAMHEALDVLEEEGVRVDAMRVRAFPFPESVKRFILDHEKVFVVEQNRDAQMRAMLIN
                     EMDIDPARLVRVLHYDGTPITARFIAHAIRERAQSAAPEAGGLPLYTELA"
     gene            complement(11244..13079)
                     /locus_tag="VGA59_01210"
     CDS             complement(11244..13079)
                     /locus_tag="VGA59_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9718324.1"
                     /translation="MHRTEISNPAYFHKVVDCQWACPSHTPVPEYIRLVAQGRYSDAY
                     MINWVSNVFPGILGRTCDRPCEPACRRSRVEENNGEHPEPVAICRLKRVAADMKDDVG
                     SRMPKVGPKNGKRVACIGAGPASLTVARDLAPLGYDVTVFDGEAKAGGFIRTQIPRFR
                     LPESVIDEETGYILNLGVHFRNNERVESMRDLMAQGYDAIFVGCGAPRGRNLQIPGRT
                     EAALHIHIGIDWLASVSFGHVKTIGKRVIVLGGGNTAMDCCRSARRLGGTDVKVIVRS
                     GFEEMKASPWEKEDAMHEGIPIINFHVPKAFVHKDGKLQGMTFEIVQAIYDDKGRRQL
                     VPTGDPDAFFECDDVLVAVGQENAFPWIERDSGIAFDEWGLPNLNKDTLQSTVPNVFF
                     GGDAAFGPKNIITAVAHGHEAAVSIDRLLNGEDIRVRPSPLTNLVSQKMGIHEWSYDN
                     DTSNDQRFKVPWAKAEKALASIKVEVELGFDVATAVKEAGRCLNCDVQTVFTESVCIE
                     CDACVDICPMDCISFTVNGEEADLRTRLKAPALNLAQALYVSGGLKTGRVMVKDEDVC
                     LHCGLCAERCPTGAWDMQKFLLKMTPAGPACRDKVAPAPRQGVPA"
     gene            complement(13340..13816)
                     /locus_tag="VGA59_01215"
     CDS             complement(13340..13816)
                     /locus_tag="VGA59_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9718325.1"
                     /translation="MKQRPFKVIGLQQIAIGGVSKSRLKRLWVDMFGLAVTGTFQSAR
                     ENVDEDICSLGAGAFKVEVDLMQPLDPDGKPAVHQTPLNHVGLWIDDLPKAVEWLTAQ
                     GVRFAPGGIRRGAAGFDICFVHPKGNEASPIGGEGVLIELVQAPPEVVKAFAALAG"
     gene            complement(13860..>14730)
                     /locus_tag="VGA59_01220"
     CDS             complement(13860..>14730)
                     /locus_tag="VGA59_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX9718326.1"
                     /translation="MIAKLVVHGEDRNDAIAKMREALNAFVIRGISSNIPFQAALLAH
                     PKFVSGDFNTGFIAEHYAKGFVAEDVPHEDPDFLVALAADVNRRTLQRAAGISGQMPG
                     HEFTVGEDFVVVVLDAAGRHRQQPASVRDFEAQSGSSAVETGGRRYEICSSEWHLGGT
                     RIRGTVNGKPFTAQVERGVGKNSLAIRIIHNGTRLDALVLTPRAAELHAVMPYKAPPD
                     MSRYVLSPMPGLLVDVAVQPGQKVQAGERVAVIEAMKMENVLFAAADGVVTKVLASKG
                     ESLVVDQPIVEFA"
BASE COUNT         2480 a         4738 c         4733 g         2779 t
ORIGIN      
        1 cctgcctaaa ggctggtcgc agggtatcca cgcgggctag cgtttccgac ccattgatgc
       61 gacgtcgtgc acgacgcctg acgctcacta tggccgtgca tgagcctcgc cgtcacgctc
      121 gctgcaagga ggctgtccgg ttgggcttta cacagctaac cagtggtttc ggtggagacg
      181 ccattcggca attcagtgcg ggcgcaggcc accttgtgca agagctcggc caggatgaga
      241 cgttcgcccg ggctcaacgc gtgcaacgcg cttgtctcgg ctttcaggat gcgctgcgtt
      301 gcttcacgcg ccagcgcaag cccttttggc gtcgcgtgca gaacctgcaa gcgccgatcg
      361 ctttcacttt gacggcgttt cacgaagccg cgcttttcaa gcttgtcgat ccaggtcgta
      421 atgttgggag aggaaaccgc cagtgcacgg gcaagacgcg cgtgcgagcc gcccggattc
      481 tctgcgatca gggtgaggag ggtgtactcc acccgccgca gtgccaaggg ttcgccgatc
      541 agttgcgtga acaggagatt cgtgacgatg gatgcctgtg cgagctggta cccgagaacg
      601 tagtggagcc tggcttcctc cagttggcca gccggtgtga tttcttctgg tttggatcgc
      661 ttggtgggca tgcggctcat tgtgatggat aggaccgtga aagcctgggg cgaactgatg
      721 aggctgccgc catgcgatcc cgcatatcgt taaggtaatt aatggtaatc aaatctctcc
      781 cacgggaaaa taccaataga gccgcgctat tagataagta gattaacgat tcgcttgttc
      841 tggctggcgc gcatgtcgcg cgctcccgac gagcggcttg ccaggagacg aacgatgagc
      901 ttcgcgcaga ataggcgccc actaagcgcc atggtcctca caattgcagt gacggtttcg
      961 gggtgcggtg atggcgggtc gagtcttcca cgactttctg cggccacgcc ggcgaccttt
     1021 gccggcggat gcggcgacct gccgggcaag ctatccctgg ccaacaccac cattaccacc
     1081 gccacgagcg tcccggcagg ctccctcacc gtggccggac aggcaatccc ggagcactgc
     1141 ctcgtcaccg gccgcatgtt cgatcgtgtc agcgcagtgg acgggcaggc ttacgctatc
     1201 ggcttcgaga tgcgactgcc catgcagtgg aacggacgct ttttccacca gggcaacggc
     1261 ggcatcgacg gcaccgtggt gacagccgtc ggtgcgttcg gcggtggacc gcttaccaat
     1321 gcgctgctgc agaggttcgc cgtcctgagc tcggacgccg ggcaccgcgc cgcccagggg
     1381 ccctttttcg gcatcgatcc acaggcgcgg ctggactacg gctaccaggc ggtgggcaag
     1441 ctgacgccga tggccaagtc actgatccag acggcatacg gcaagggccc ggaccgctcg
     1501 tatctcggag gatgttccaa cggcgggcgg cacgccatgg ttgcggcttc gcgctatgcc
     1561 gaccagtacg acggcatcct ggccggcgcg ccgggctaca acctgccaaa agcggcgatc
     1621 gccaacatcg cgggcggcca gctgtacgca tcggtggcga ccaatcccgc cgacatctcc
     1681 accggattca ccacggccga gcgcacgctc gtggccaacg ccgtcctgtc tcgatgcgac
     1741 gccctcgacg gcgcaacgga tggtctggtc caggacgtgg cggcctgcca gtccgcattc
     1801 agcctcgatc gggacgttcc cacctgcacg ggcgcgcgtg atggcacttg tctcagttcg
     1861 gcgcagaaga ccgccatcgg gaagatattc agcggcccga ccaccagcag cggtgcgttg
     1921 atctattcgt cctttcccta cgacggcgga ttgtccggcg ccggaatacc gttctgggaa
     1981 ttcactgcgc cgctgcagct ggactcaggc gcggtgggcc tgatcttcaa ggtgccgccg
     2041 gaaagcgcgg aaggcttcaa cggcccggcg tttgccctgt cggcaaacct ggatacgctg
     2101 gtggcgcaga tcaacgcgac caacagcctt tacacggagt ccggcatgac tttcatgacg
     2161 ccgccgaatc ccactaacct ttcagttctg aagaaccgcg gcgccaagat gctcgtctac
     2221 cacggcgtga gcgatccgat cttctccgtc aacgacacgg agacctggta caaggggctg
     2281 cagtcggcca acggcggcga tgcatcggaa ttcgcgcggt ttttccgcgt ccccgggatg
     2341 gggcattgct cgggtgggcc ggcgacggac cagttcgacg tgatcactcc acttgtcaat
     2401 tgggtggagc agggtcaaga gcccgcgcaa gttatcgcca ccgcgcgtgg ccccggcaac
     2461 gccgcagtgg cgaacccgga ggttccgtca agttgggctg ccggccgcac tcggccgctc
     2521 tgcccctatc ccaaggtggc tcgctatgcg gggagcggca gcctggaagt ggccagcagc
     2581 ttccgatgcg agtgatggcg caagctgcat cttctctgcg ttcagcgcgg gcggggtgct
     2641 ccccgcctgc ttggttcgac gtccggcaag cgccgccttg agtgaagacc tggcctcgta
     2701 aacacgttga atgaagagtc gtagcgctac tgctgtacag ggccggcggc agcgttcctc
     2761 gaccaccagc gcccggcctt tattgcagcc gtaaatgttg tgtattgcga cctacgcatg
     2821 gacgcaatgt cgagccgaca ctatactgcc gtcggcttaa ccactttgcc gaaaccagcc
     2881 tcagaatttt cgacggaatg aacctgtcca cagctgccgc cacttccacc gggactaaac
     2941 ccatcactca cgtcttagga gaatcatgaa ctctacgatg gatcgccgta cggcgcttat
     3001 gactggtgct gcccttgccg gggcgtcgat gatgccggcc tgggcgcagg ccaagccggt
     3061 cctgcgtttc gcagcggtgt tctcggacaa ggacatccgg gcggacatga tgcggatgct
     3121 cgccaaggac ctcgaagcgg acttcaaggt ggacctgttc ctcaactcga cgttgttcaa
     3181 gcagggtacc gaactggtgg cgctgcagcg cgacaacctg gagctcggca acatcgggcc
     3241 tcaggacttt tcgaagcaga tcccggcctg gtcgatcctt acctcggcgt acctgtttcg
     3301 cgatgccaac cacctgaacg cgttcttcgc gagcgacctg ggcgcacaga tgaagaagat
     3361 ggtcgaggac caggtcaagg tgaagatcct ggggccgacc ttcttcggta cccggcaggt
     3421 gggcctgaag gtgaagaaga agatcaacac gcccgccgat atggccggca tcaagctgcg
     3481 catgccgccc ggcgatgcgt ggcagctgct ggggcgttcg ctcggcgcca atccgacgcc
     3541 catggcttac gccgaaacct ataccggttt gcagaccggc gctatcgacg gccaggacaa
     3601 tcccctgccc aatgtgcaga acatgaagtt caacgaggtg atgtcgcaga tcgtgctcac
     3661 ctcgcacctc atcggcttcg acacgctcgc catcaacctg aaaacctggc aggccatgtc
     3721 gcccgccaag cagaagtcct tccaggccgc agtcgacaag gcactcgcct ggagcacggc
     3781 agaacatctg aaacgcgaag ccgagctgat cgcgggcttc aaggcccagg gtctggacgt
     3841 gtacacgcca aacgtcaacg cgttccgcga atatgcgcag aaggtttatc tggaatcccc
     3901 tgaagcgaag acctggcccg cgggcatgct cgacaagatc aatgccatga aataaggctc
     3961 gcagtcccgc tggtggcgcg cgctgccggc gggaatcatc cgggtccgac gcagcacacc
     4021 aacaatggcc tcgaaatccc tggcaggcaa gatcggggct ttttccaaac gattcgccga
     4081 agtcgtcgcc gccgggctga tggccgccat cttcgtttcc ttcatcgtcc agatcgtctt
     4141 ccggtacgta ttcaattttc cggtcggctg gacttccgag ctttcggtgg ccgcctggct
     4201 ttggctcgtc ctctggggcg ccggcttcgt tctcaaggaa gaggaagaga tccgctttga
     4261 cctcctatcg gggggcgcgg gacgccgcac gcgcttcgtg atggcaatcg tgaccggggt
     4321 tgccatcatc gtgctgtttg cgatgtcgct tcccgcggcg tattcctacg tcaccttcat
     4381 gaaggtcgaa agcacttcct atctcaagat tcgattcgac tggctgttct cgatctacct
     4441 gatgttttcg gtcgcggtaa tcgtccgcta tctctggaag ctcgggcagt tgctgcgggg
     4501 ccggatttcc gagccccctg aagcaaccaa gatgagctcc gccctgtgaa tccggccagc
     4561 cctttttcgc tcgccgtcat ggcgctcgtg gcgctcagcc tgctcgggtt gccaatcggt
     4621 cacgcgatga tcggcggctc gatcctctac ctctatctcg cgggtctgga catgggcacc
     4681 gcggccgagc aactgctcaa cggcatgtat tcgagctacc tgctgctggc gattccactg
     4741 ttcatctttg ccgcgacgat catgagcacg ggcagcatcc tggaccggct gctgcgcttc
     4801 tgcaacgcgc tcgtcgggcg ctttcccggc ggcctggcgc aggtcaatgt tgtgcagagc
     4861 atcgtcttcg ccagcatgtc gggttccgcg cttgcagatg ccgcgggctc cggcaagctg
     4921 atgcaggcgc tgatgacgag ggaaggaaaa tacacgaaga gcttcgccgc cgcgctgacc
     4981 gccgtgtccg ccgtcatcgg cccgatcctg ccgccgtcga ttccgctggt tctctacgcg
     5041 cttgtttccg acgcgtccat cggctacctt ttcattgcgg gcgttttccc tggtctgctg
     5101 ctggctgccg tgcaattggc cctgatcgcg ctcatggcaa aacggcggaa cttcccggtt
     5161 gaggcagcgg tgccgctgcg cgaactgccg ggcatcgccc gggaggcgtt tccggcgttg
     5221 ctgatgccgg tcatcctgct gggcttcctc tacagcggcg tgacgacacc gaccgaagcg
     5281 gctgccgtgg cggccgcgta cgccctgctg atttcgacgg cgctctaccg cagcgtcagc
     5341 tggcccgacc tctaccagtc gctcgtttcc agtgcgcgcg tcactgtgtc gatcggcatg
     5401 ctgatcgccg gtgcactggt cttcaactac gtgatcacca tcgagaacat tcccgggacg
     5461 ctcagcaccc tgctgaaggg gttcgagctg tcgccgcttg gtttcctgct gatcgccaac
     5521 gtcgtcctgc tggtcctggg ctgtttcctc gagggatcga cgatcatcct ggtgatcctt
     5581 cccgtactcc tgccgacggc ccaggcactc ggggttgatc ccgtgcactt tggcatcgtg
     5641 gccgttacga acatcatgat cggcctggtc accccaccct atggcctgct gctgttcatg
     5701 atggtcaaga tcgcggacgt gccgttgaag gacctggtca tcgaggtcct gcccttcctg
     5761 ggggtcatgt tcgtcgcgct cgcggtgatc acgctgttcc cggacttcgt gctctacctg
     5821 ccccggctcg tcggctacaa gggttaggca ttgatccacg cggccaccgg gtcgccaatg
     5881 ctcagcgagc tgataatggc gatgtttccg agcgggccgc gcaccggcag cggctttatg
     5941 gtttgcccgc gcacacgcac tttcagggtg ctggcctgat tggacgcctt gtatggagga
     6001 atacctgtgg cgaatgtgat agaccgagaa attcacatcg atgcgatcaa tccgctgggc
     6061 ctgaacggca tcgagttcat agagtacgca acttccaggc cccaggcggt ggggcaggcg
     6121 ctggagtcga tgggcttccg gccgatcgca agacaccggt cgcgcgaagt gctgctgtac
     6181 cggcaaggcg ccatgaacgt ggtgatcaat gcccacggca accccagccg gggcagcctt
     6241 caactggggg atacacccgc gatcggcgcc gtggcgttcc gggtccagga cgccgccatg
     6301 gcgtacgcgc gcgcgctgga ccgtggcgcc tgggccgttc cgacgcacgt cgaagtgatg
     6361 gaactcaata tcccggccat ccatggcgtg ggcagcagca ggatctattt tgtcgatcga
     6421 tatcgtgagt tctcgatctg ggatgtcgac ttcgtgccca tcccgggcgt ggaccagcac
     6481 ccgccggcat tggcgggctt gcattggttc ggcctcgtgc aatacatcga ctacgaccgc
     6541 atggccgact ggtgcgagtt ctatcacgag ctcttcgggt tcgccgagct gccggacgac
     6601 gaacgcttcg gcatcatgcc caatggcaag atcctggcca gcccctgtcg gacctttttt
     6661 ctgcaactgg tcgaaccccc gcccggcctc gacgatgtgc aatgcgagga gatgcttcag
     6721 cgcatcggcc tgggaacgcc cgatgtaccg gccgccgtca cggcgctgcg cgggcgcggc
     6781 gtggaatttg tcgagggcgc gcacgtccac accgatgttc gtggcgccct cacgaagccg
     6841 ttggcgggca tgatgttcga gctggtgcac gacgagcggg cagcgtcatg acccagcgca
     6901 gcggcaactt cgacgacttc ggcatggcca ccgtgaccct cgccgggccg ctggagggca
     6961 agctgaaggc aatccgcgag gccgggttca gccaggtcat gcttgaagcc agcgatgtgg
     7021 ccgggcaccc cggaggcatg gacgcggccc tggcggccgt gaggtcgagc gggctgcgcg
     7081 tcaccggatt ccaatggctt cgcgacttcg agggtctcgc cggccgccag cgcgattaca
     7141 aggtggaagt cgccaaggcc atgctgcaga tggcgcatgc gctgggcgcc ccgatgctgc
     7201 tcgtggcgtc gacgaccaac agcattgcgg cgcaggacct ggatgccgtc gcgcgcgatt
     7261 tgcgcaagct cgccatcctc gccgtgcctc tggggctgaa ggtcgcctac gaaggcatga
     7321 gctggagccg cacggtgacc gatttcgtcg cggcctggga cgtggtctgc cgggccgacg
     7381 cccccaatct ggggctggcc atcgacagcg ctcacacgct ggtcagccag acgccactgg
     7441 aggaactgga attgatcgag ccccacagga tcttcatcgt gcggctggcc gatttcatgg
     7501 aaccgcaggc gcgttccgac gagaagagca tgatgacccg gacgcagttg cgcgtcttcc
     7561 cgggtgaagg tctgcacagc gccgcggtgg ccgacctggt gacgaggctg gcggcactgg
     7621 gctacagcgg agattacagc ttcgaagttt tcaacgcgga ttaccagcaa ctgcccccgc
     7681 cgctggtggc ggaacgcgcc cgcgccgcct cgcagtggct gggcgaggac gtgctgcgcc
     7741 gttcagtccc gttgcccagc agcatgcggt tgcgccagaa ttccagcccc agggactgaa
     7801 gcgccggcca tcgttcgcca agcgccggtc aagaggatac ggccggctgg actcagccca
     7861 gcgcgtcgtt cacctgttca ttgaactccg ccatgctgag ggcgttgccg atttcgcgcg
     7921 gcagcgcgtc acgcaccgaa aagaccccca ggatcttcga tccatcgcca atgacgggca
     7981 agtgccggaa tccgcgctcg atcatgatga cgaccgcgtc ggagaccagt gtctccggcg
     8041 gcacgcacaa gggattgggc gtcatcacgt cgcacacctt ggtcttggcg ggatcgagcg
     8101 ccttggccag cacgcgcgtc atcaggtcgc gttcggtgac gatgcccagc aaggtgcccg
     8161 gcaggtccac gacgagcacg ctgccgcagc tggcgcgcgt catgacgcac gctgcctccc
     8221 agacactggc gttggggccc aggctgagaa catgtttctg ggacatcgat tggaaaacgg
     8281 tgcgttcagt catgatgcgc ccccttgcaa gtgaagttat ggaaccgctc ccgccgtatt
     8341 ccggctagcg cagggcggcg ttgatcgcgt cgagcgcctt ggaacccggg caaagctgcg
     8401 gggcgccgag cgcggccagc ggcttctggc tagtgttcag cgccatgtgc agatcggtcg
     8461 ccaccgggtc cacgtagagc agccccgtga cgatctcgcc gcgagcctgg tgcgtctgca
     8521 tgtagctgat ggcgccgtag cgatccgtcg ggtcgtagtc ctggtggagc ttgcgtaaac
     8581 gcagtttcga gccgtcatgc tgctgaactt cgatcaccgc gcccggctca tactgggccg
     8641 tgatttcgcc gcgcggcgtg atgaagtcga tgcggctcac cgcctcgttg tgctggcgca
     8701 cgtagtcgta gctcttggtg ctgccgccgt ggttgttgaa ggtcacgcag gggctgatca
     8761 cgtcgatgaa cgcggcgccg ccatgcgcca tggcgccctt gacgagcggc accagttgcg
     8821 ccttgtcgcc cgagaagctg cggcccacgt aggtggcgcc cagctgcagc gccaatgcga
     8881 cgaggtccac gggactgtcg ctgttgacga cgcccttctt gctcttggag ccgcggtcgg
     8941 ccgtggcgga aaactgtccc ttggtcagcc cgtagacccc gttgttttcc acgatgtagg
     9001 ccatgcagac gccgcggcgc atcgcgttgg cgaactggcc caggccgatc gacgcggagt
     9061 ccccgtcgcc ggagacgccc aggtagagca ggtcgcggtt cgccaggctg gcgccggtca
     9121 gcacggaggg catgcggcca tgcacggtgt tgaagccatg cgaggcgccc aggaagtaat
     9181 ccggcgtctt ggagctgcag ccgatgcccg agagcttggc gacgcggtgc ggctcgatgt
     9241 cgagttccca gcaggcctcg atgatggccg cggaaatgga atcgtgcccg caccccgcgc
     9301 acagcgtgga gatgcggcct tcgtaatcgc ggcgggtata gccgaccttg ttcgcggtca
     9361 gcgacggatg ccgcaggcgg ggtttggcga tgtaagtcat gcgagctccg tgtacagggg
     9421 caggccaccg gcttcgggcg cggccgattg cgcgcgttcg cggatggcat gggcgatgaa
     9481 acgggcggtg atcggggtgc cgtcgtagtg cagcacgcgc accagccgcg ccgggtcgat
     9541 gtccatctcg ttgatcagca tggcgcgcat ctgcgcgtcg cggttctgtt cgaccacgaa
     9601 caccttttca tggtccagga tgaagcgctt gaccgattcg gggaacggga aggcccgcac
     9661 cctcatcgca tccacccgca cgccctcttc ctccagcacg tccagggcct cgtgcatggc
     9721 cgggctggtg gagccgaagt agatcacgcc cagcggcgtc ttctgcgcgg cgacgcgcac
     9781 gaccggctgg ggcaccagct gcgccgcggt cttgaacttg cgcagcagac gctccatgtt
     9841 gtaggtgtag tccgggccgc gctcggagta gcgggcatag ggatcgcgcg tggtgccacg
     9901 ggtgaagaaa ctgcctttgg tcggatgcgt gcccggcagc gtgcgccagg gaatcccgtc
     9961 cccgtccacg tccttgtagc ggccgaagtc cttgccggcc tcgagctcgg ccgccgtcat
    10021 gaccttgccg cggtcatagt cgcgcgcgtc atcccacacg aacggcttgg cgaggcgctg
    10081 gttcatgccg atgtccaggt cggtcatcag gaagaccggc gtttgcaaac ggtccgcgag
    10141 atccagcgcg gcagccgcat gctcgaagca ctcgtgcggg tcctgcggga acagcagcac
    10201 gtgcttggtg tcgccatggg aggcatacgc gcaggcgatc aggtccgatt gctgcgtgcg
    10261 cgtgggcatg ccggtggacg ggccgccacg ctgcacgtcg atgatggtga ccggaatctc
    10321 ggcgaaatag gccaggccga tgaattcggt catcagcgat atgccgggtc cggaggtcgc
    10381 ggtgaaagcc cgcgcgccgt tccatcccgc cccgatcacc atgccgatgg aggccagctc
    10441 gtcttccgcc tgcacgatgg cgtagttgta acggccgctc cccggatcca cccggtactt
    10501 ctcgcagtac ttctggaacg cctcgggcac ggacgaggaa ggcgtgatgg gataccaggc
    10561 cgcgacagtg gccccgccat acacgcaacc cagcgccgcg gcactgttgc cgtccacgaa
    10621 gatctggtcg ccgaccttgt cggacctgcg cacgcggatg cccagcgggt actcgagatg
    10681 ctcggtcacg aagtcccggc ccaggtgcag ggcgcggata ttggagtcca gcagccgctc
    10741 cttgcccttg tactgctcgg cgaagagttt ctcgaacacg tcggcatcga tgcccagcag
    10801 caccgacagc gcgccgacat agatgatgtt cttgaacagc tggcgctggc gcgcgtcggt
    10861 gtacaccgcg ttggtgattt cggtgagcgg catgccgatc acgtgcacgt cttcccggaa
    10921 cctggaggcc ggcagcggcc gcgtgctgtc gtagaacagg tagccgccgg actcgatttc
    10981 ggcgacatcg gtgtcccagg tctgcgggtt catggcgacc atcatgtcca cccccccgcg
    11041 ccggccggcg tagccctttt cgcagacccg tgcctcgtac caggtgggca ggccctggat
    11101 gttgctgggg aagatattgc gtggactgac cggcacgccc atgcgcagga tcgccttggc
    11161 gaagagttca ttggcactgg ccgagccgga gccgttgacg ttggcgaact tgatgacgaa
    11221 atcgttgacc gcttcgatct tcttcatgcc ggcactccct ggcgcggcgc cggagcgacc
    11281 ttgtcccggc aagcgggccc cgcgggcgtc attttcagca ggaatttctg catgtcccag
    11341 gctcccgtcg ggcaccgctc cgcgcacagg ccgcagtgca ggcagacatc ttcatccttg
    11401 accatcaccc gccctgtttt caggccgccc gatacgtaca gcgcctgggc gagattgagc
    11461 gccggcgcct tcaggcgcgt tcgcagatcg gcttcctcgc cgttgaccgt gaagctgatg
    11521 cagtccatcg ggcagatgtc gacgcaggca tcgcattcga tgcagacgct ctcggtaaag
    11581 accgtctgca cgtcgcaatt caggcagcgc ccggcctcct tcacggccgt cgcgacgtcg
    11641 aaccccagtt cgacctccac cttgatgctg gccaaagcct tctcggcctt ggcccagggc
    11701 accttgaagc gctggtcgtt gctggtgtcg ttgtcgtagc tccactcgtg aatgcccatt
    11761 ttttgcgaca cgaggttcgt cagcggcgac ggccgcacac ggatgtcctc gccattgagc
    11821 aggcggtcga tcgacaccgc ggcctcgtgg ccatgcgcca cggccgtgat gatgttcttg
    11881 ggtccgaagg ccgcgtcgcc gccgaagaat acgttgggca cagtcgactg cagcgtgtcc
    11941 ttgttcaggt tgggcaggcc ccactcgtcg aaggcgatgc cgctgtcgcg ctcgatccag
    12001 gggaaggcat tctcctgccc cacggcgacc agcacgtcat cgcattcgaa gaacgcatcc
    12061 ggatcacccg tggggaccag ctggcgccgg cccttgtcgt cgtagatcgc ctgaacgatc
    12121 tcgaaggtca tgccctggag cttgccgtcc ttgtgcacga acgccttggg cacgtggaaa
    12181 ttgatgatgg ggatgccctc gtgcatcgcg tcctcttttt cccacgggga agctttcatt
    12241 tcctcgaagc cgctgcgcac gatgactttc acgtccgtgc cgcccagccg ccgcgccgag
    12301 cggcagcagt ccatcgccgt gttgccgccg cccagcacga tcacgcgctt gccgatggtc
    12361 ttgacgtgcc cgaaggacac cgaggccagc cagtcgatgc cgatgtggat gtgcagggcg
    12421 gcttccgtgc ggccgggaat ctgcaggttg cgcccgcgcg gtgcgccgca accgacgaag
    12481 atcgcgtcgt agccctgggc catgagatcc ctcatggact cgacgcgctc gttgttcctg
    12541 aagtggacgc ccaggttgag gatgtacccg gtttcctcgt cgatcaccga ctccggcaga
    12601 cggaaacgcg ggatctgggt gcggatgaag ccgccggcct tggcctcgcc gtcgaacacg
    12661 gtgacgtcat agccgagcgg ggcgaggtcg cgcgccactg tcagcgacgc cggcccggcg
    12721 cctatgcatg ccacgcgttt gccgttcttc ggcccgacct tgggcatgcg ggagccgacg
    12781 tcgtccttca tgtcggcggc cacgcgcttg aggcggcaga tcgccaccgg ctccggatgc
    12841 tcgccgttgt tctcctcgac gcggctgcgc cggcaggcgg gctcgcaggg acggtcgcag
    12901 gtgcggccca gaatgccggg gaaaacattc gacacccagt tgatcatgta ggcatcgctg
    12961 tagcggccct gagcgaccag gcgaatgtat tcaggcacgg gcgtgtggga cgggcaggcc
    13021 cattggcaat cgacgacctt gtggaaatag gctgggttgg aaatttccgt tcgatgcaaa
    13081 actgtctcct ggtgccggtt cgcctggaac cagcggcacc cctgttgggg aatcattgaa
    13141 ccgcgcccgc cttgccccgg gtcgggaagc cgcggacctg taaacagtct acgcgagcgc
    13201 ccaccagcag agctttcggg caatcccgaa gcgcgtcagt gtagcgtcag cagctgatcg
    13261 gagcggccct cctgcggcgc cggggcctgc cgtcgatact catcaattgg aaacggacct
    13321 ggcggctgga cagtcgcgct tagcctgcca gggcggcaaa cgccttgacc acctcgggcg
    13381 gggcctgcac cagttcgatc agcacgcctt caccgccgat gggcgaagcc tcgttgccct
    13441 tgggatggac gaaacagatg tcgaaacccg ctgcgccgcg cctgatgcct cccggcgcga
    13501 agcgcacgcc ctgggctgtc agccattcca ccgctttcgg caggtcgtcg atccaaaggc
    13561 ccacatggtt gagcggcgtc tggtgcacgg cgggtttacc gtcggggtcg agcggctgca
    13621 tcaggtccac ttcgaccttg aacgcgccgg cacccaggct gcagatgtcc tcgtccacgt
    13681 tctcgcgcgc actctggaac gtgcccgtga ccgccaggcc gaacatgtcg acccagagtc
    13741 gcttcaaccg gctcttggat acgccgccaa tggcgatctg ctgcagaccg atgaccttga
    13801 acggacgctg cttcatgaaa aggccctgaa gttgatatgt gtatcggccg gcgcactcat
    13861 caggcgaact cgacgatcgg ctgatccacc acgaggcttt cgcccttgct tgccagcacc
    13921 ttggtgacga cgccgtcggc ggcggcgaag agcacgttct ccatcttcat ggcctctatc
    13981 acggccacac gctcgccggc ctgcactttc tggcccggct gcaccgccac gtcgaccagc
    14041 agtccgggca tcggcgagag aacataccgg ctcatgtcgg gcggcgcctt gtagggcatc
    14101 acggcatgga gctccgcggc gcggggcgtg agcaccaggg catcgagccg ggtgccgtta
    14161 tggatgatgc ggatcgccag cgaattcttg cccacaccgc gttccacctg cgccgtgaac
    14221 ggcttgccgt tcaccgtgcc gcggatgcgg gtgccgccta ggtgccactc gctgctgcag
    14281 atctcgtaac gccgcccgcc ggtttccacg gcgctggagc ccgactgcgc ctcgaaatcc
    14341 cgcacgctgg ccggctgctg ccgatgccgg cccgcggcgt cgagcacgac cacgacaaag
    14401 tcctcgccca ccgtgaattc gtgtcccggc atttgcccgc tgatgccggc cgcccgctgc
    14461 aaggtgcggc ggttcacatc ggccgcgagc gccaccagga agtcggggtc ctcatgcggc
    14521 acgtcctcgg cgacgaatcc cttggcgtag tgctccgcga tgaatccggt gttgaaatcc
    14581 ccggagacga acttggggtg cgccagcaac gcggcctgga agggaatatt gctggagatg
    14641 ccgcgaatga cgaaggcgtt gagcgcctcg cgcatcttgg cgatggcgtc gttgcggtcc
    14701 tcgccgtgca cgacgagctt ggcgatcatc
//
LOCUS       DASWIY010000032         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_764499, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000032 DASWIY010000000
VERSION     DASWIY010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764499"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VGA59_01225"
     CDS             <1..414
                     /locus_tag="VGA59_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718327.1"
                     /translation="LPRLLARTNARHAPWLAVLVAAGAAAILTYIGKAEQLVVLLVFG
                     ATTCYLLILASHWRLRRVAPEMPRPYRSPAGRFIPALGMLLAALAFVACFLADIKWSM
                     LGAALLLLSMLYFRVTGPTRAINHIPINHIPKVTQ"
     gene            411..>1664
                     /locus_tag="VGA59_01230"
     CDS             411..>1664
                     /locus_tag="VGA59_01230"
                     /inference="COORDINATES: protein motif:HMM:NF005682.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX9718328.1"
                     /translation="MRKATPVDPRTTADWQAADNTRFLHPFTDYKQLHAEGARIQERA
                     SGIHVWDTDGNKIIDGLAGLGCVNIGYGRPELAHAAARQMERLSFSQSFFRTSNQPAI
                     ELAERLVSIAPKSLAHVFYSSSGSEANETCLKLVRRYWDLVGRPEKRFIIARASAYHG
                     STIATSVLSGLPDMHAGMGSMTLDGIERIDAPYWYVHNKGESIDAFGTRAAGWLEEKI
                     LALGADNVAAFFAEPMQGAGGAIIPPLTYWPEVQRICRKYDVLLSVDEVVTGFGRTGK
                     WFGSEYFGIADMDLMAVAKGITSAYIPLSAALVSDRIADVLIERGGEFWHGFTHSGHP
                     VACAVALENIRLLQDERIIEHAAAVTPQFAAHVAALADHPLVGDARSCGLFAGLQLVA
                     DKASRTMFDATMQVGDRCSREALRRG"
BASE COUNT          305 a          489 c          521 g          349 t
ORIGIN      
        1 ttgccccgat tgctggctcg aacgaatgcc cgccacgcgc cgtggctcgc ggtgttggtc
       61 gctgccggtg cggctgcgat cctgacgtac atcggcaaag ccgaacaatt ggtggtcctt
      121 ctggtcttcg gagcgactac ctgctatctg ctgattttgg cgtctcactg gcgactgcga
      181 cgtgtcgcgc ccgagatgcc gcgtccttac cgcagcccgg cggggcgctt cattccggcg
      241 cttggaatgc tgctcgcggc cctggctttt gtggcctgct ttctcgccga catcaaatgg
      301 tcgatgctgg gtgcggcatt gttgctgctc tctatgttgt actttcgcgt gaccggtccg
      361 actcgggcaa tcaatcacat tccgatcaat cacattccga aggtgacaca gtgagaaaag
      421 caaccccggt ggatccacgc actaccgcag attggcaggc cgcggacaac acccgcttct
      481 tgcacccgtt cacggactac aaacagctgc atgcggaagg tgctcgaatt caggagcgcg
      541 cctccggaat ccatgtctgg gacacagacg gcaataagat catcgacggc ctggcggggc
      601 tcggctgcgt gaacattggt tacgggcggc ccgagcttgc gcacgcagcc gcacgccaga
      661 tggagcggct gtcattcagc caatcgttct ttcgaacttc aaaccagccg gccatagagc
      721 tcgcagaacg actggtttcg atcgcgccga aatctctagc tcacgtcttc tattccagct
      781 cggggtccga agccaacgag acctgcctca agctcgtgcg ccgctactgg gatctggtcg
      841 gccgccccga gaagcgtttc attatcgcgc gcgcgagcgc gtatcacggc agcaccattg
      901 ccaccagcgt cctttcagga ttgccggaca tgcacgctgg catgggctcg atgacgctgg
      961 atgggataga gcgcatcgac gcgccttact ggtacgtgca caacaaaggt gaatcgatcg
     1021 acgcgttcgg tacgcgcgcg gcgggctggc tggaggaaaa aatcctggca ctgggtgccg
     1081 acaacgtcgc cgcctttttt gcagagccga tgcaaggtgc gggcggcgcc atcattccac
     1141 cgctgactta ctggccggag gtacagcgta tctgccgcaa gtacgatgtg ctgctgtcgg
     1201 tggacgaggt ggtgacggga ttcggtcgca caggaaagtg gtttggcagt gaatacttcg
     1261 ggattgcgga tatggacttg atggccgtgg ccaagggtat tacctcagcc tacatcccgc
     1321 tgtccgccgc tctggtgagc gatcgaatcg cggacgtgct gatcgagagg ggaggggaat
     1381 tctggcacgg tttcacgcac tcgggtcatc ctgtggcctg cgcagttgcg cttgaaaaca
     1441 ttcgcctgct acaggatgaa agaatcatcg agcatgccgc agccgttacg cctcagtttg
     1501 cggcacacgt ggcggcgctc gccgatcatc cgttggtcgg tgacgcacgc tcgtgcggct
     1561 tgttcgccgg gctgcaattg gttgccgaca aagcgagtcg tacgatgttc gatgctacga
     1621 tgcaggtcgg cgatcgctgc agtcgagagg cgctcaggcg cggg
//
LOCUS       DASWIY010000033        12848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3349339, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000033 DASWIY010000000
VERSION     DASWIY010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 12848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12848
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3349339"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VGA59_01235"
     CDS             complement(<1..572)
                     /locus_tag="VGA59_01235"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX9718329.1"
                     /translation="MQVVPHPDFMVFTGNANPDLAAEIAGHLGISLGAAHVGRFSDGE
                     VTVEISTNVRARDVFVVQSTCAPTNENLMELLIMVDALKRASAERISAVIPYFGYARQ
                     DRRPRSTRVPISAKVVANLLQTVGVARVLTMDLHADQIQGFFDIPVDNIYASPVLLGD
                     LRHKNYDDLIVVSPDVGGVVRARALAKQLDT"
     gene            complement(679..755)
                     /locus_tag="VGA59_01240"
     tRNA            complement(679..755)
                     /locus_tag="VGA59_01240"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(719..721),aa:Gln,seq:ttg)
     gene            complement(777..1685)
                     /gene="ispE"
                     /locus_tag="VGA59_01245"
     CDS             complement(777..1685)
                     /gene="ispE"
                     /locus_tag="VGA59_01245"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX9718330.1"
                     /translation="MKALYDVPAPAKLNLFLHVTGRRPDGLHLLQSVFMLIDWSDTLN
                     FELRTGRALSREDLGEPLPADDLVLRAARSLQAASGATAGAHIAIDKRVPAQAGLGGG
                     SSDAATCLLALNRLWRLGLTVPELARIGLALGADVPFFLHGRNAWVEGIGERLKPVVL
                     PAARFAVAKPPQGLSTGLVFNDLRLNRSDDTAIISGFAAKQYGESGRPSLEDFMENPF
                     GFGRNDLQPVAQKLCPGVTQALQWLGSQGLAGRMTGSGSGVFSQLPQGRSLQAAPAGW
                     QVRECGNLEAHPLVGWAPSDDSSVGG"
     gene            complement(1696..2190)
                     /locus_tag="VGA59_01250"
     CDS             complement(1696..2190)
                     /locus_tag="VGA59_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEX9718331.1"
                     /translation="MHRPWARWAIALAIAVAAGCASPPRTAGPTDALSGPWSGRLALE
                     VEENQPQSFSAGFELKGTATSGELALYNPLGATMAVLAWAPGSARLLSNGQAREFESV
                     ESLVAHATGAAIPVAALFDWLRGIDTPVPGWKADLSLLGQGRLRAQRLQPPPRADLRV
                     VLER"
     gene            complement(2193..3935)
                     /locus_tag="VGA59_01255"
     CDS             complement(2193..3935)
                     /locus_tag="VGA59_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9718332.1"
                     /translation="MKRFSLAAGALLLLALAQAACAQLPAAGDPDGAAATTPLDAELF
                     YQLLLGEINARGVEPAAGYSLILDAARRTNDPALYQRAVEVAFQSRSGDAALQAARAW
                     KQAFPESREANRYVLQILVALNRIADSAEPLQTELALAGPQDRESVLAAIPRSYSRVS
                     DKKLAAEVVEKALGEYLAQPATAAAAWTAVGRMRLLAGDNTGAVDAATRGQAANAQAE
                     APALLAMELMDPKLPQAEAIVRRYMAGQPLPELRMGYARALLDAQRYAEASQQLQLVT
                     AQKPDYPEAWLVQGTLQLQDNQDAAAEASLKRYLELAQSQRTGEERSRGQAQAYLSLA
                     RIAEKRKDYPLASAWLDKIENPQDLVAAQNRRASILARQGKLEQARQLLRSLPERNAD
                     DTRMKITAEVQLLREHKQWKAAYDLLAQATAKAPLELDPDLIYEQAMVAEKMGNLADM
                     ERLLRGVIANKPDYHHAYNALGYSLADRNMRLPEAKELIQKALTYAPEDPFISDSLAW
                     VEFRMGNRDEALRILSAAYKMRPDAEIAAHLGEVLWSLGQRERAQAIWKEGLLLNSES
                     ETLQETLKRLRVKL"
     gene            3975..4790
                     /gene="mutM"
                     /locus_tag="VGA59_01260"
     CDS             3975..4790
                     /gene="mutM"
                     /locus_tag="VGA59_01260"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX9718333.1"
                     /translation="MPELPEVEVTRLSLADRIAGARIEAVRVGKPLRWPLGLQADRLV
                     GRTVRGVRRRGKYLLMDLDEGLLLLHLGMSGSLSFAPRPPLAGAHDHFDLVTSRGILR
                     LNDPRRFGAVVYAAGENSPEASKLLGRLGVEPLSEAFGLDLFHAGLKRRHAPVKQVLL
                     AGDLVVGVGNIYASEALFLAGIRPTLRASRISRPRAERLRAAVREVLARAVAVGGSTL
                     RDFSNAHGEAGYFQLEAMVYDRAGEPCRICGTPVKSIRQGQRASYFCPNCQKS"
     gene            4840..6804
                     /locus_tag="VGA59_01265"
     CDS             4840..6804
                     /locus_tag="VGA59_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX9718334.1"
                     /translation="MGTSFNDQFDQHGVWRREFALRLKLLSEWLKDHELLDAAVEERL
                     RRLETQIRSEKVMVAFVAEFSRGKSELINAIFFAGYKRRIMPASAGRTTMCPTELGYD
                     VGVPPCLRLLPIETRLQPQPLMEWRMVPEKWIRVVLDVNDPAQLAKAFEKVAEVRHVT
                     KDEAKALGFWHDDQPDDNPLVGADGKVEVPRWRHALINIAHPLLKQGLVILDTPGLNA
                     IGAEPELTVNLIPQAHAVVFILAADTGVTKSDLAIWREHLITEADNIESRLVVLNKID
                     TMWDSLSTPAQVQAEIDRQRATSAEILGLPLEQVIPVSAQKGLVAKVTGDTALLQASE
                     LPVLERALGQGVMGQRQRILRSAVESGIGELRVEAARAIGIRRRDLAEQMVELRGLRG
                     KNIAVIRHMRSRIEQEQREFNVSGARIHAVRFVHLKLLHEVFVQLGTATLKVEMAELT
                     TALRQPGIKLGVRKAYAQTFDRLRAVLRKAQTTSAEIQSMLTGTFRQLNAEYGFSLQA
                     PREPELDRYVRDLDLVERSHNQYLGVTNVFRLAQPEFADRLVRALATRLRVVYESALG
                     EVELWNKSAAAQLDAQLRERRRTFTRRLEAIERIQQAASGLDDRIGEIGQQERALDDL
                     NAKLTELTDHLVNAPGSAGHQPAEPTLQRA"
     gene            6801..7829
                     /gene="mutY"
                     /locus_tag="VGA59_01270"
     CDS             6801..7829
                     /gene="mutY"
                     /locus_tag="VGA59_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX9718335.1"
                     /translation="MSFAGRIVRWQRTHGRNHLPWQNTRDAYRVWLSEIMLQQTQVAT
                     VLDYYARFIARFPDVRALAAAEVDEVLALWSGLGYYSRARNLHRCAREVVALHGGEFP
                     GNAQALRTLPGIGRSTAAAIASFCFGERVAILDGNVRRVLTRVLGFGADLASASHERQ
                     LWERAQALLPQRSARQTMPRYTQGLMDLGATICLARKPSCLLCPVRELCVAHREGAPE
                     NYPVKTRKLKRGAQSLWLLRAQAGDGSVWLEKRPAQGIWGGLHCLPVFESRDALQAAV
                     PAAARVRLQDETPFLHVLTHKDLHLHPVGAAISRTSMRGAQGGWFGSHEWAQLGLPAP
                     IRRLLARG"
     gene            complement(8028..8918)
                     /gene="rapZ"
                     /locus_tag="VGA59_01275"
     CDS             complement(8028..8918)
                     /gene="rapZ"
                     /locus_tag="VGA59_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX9718336.1"
                     /translation="MTLELVLITGMSGSGKSVALRALEDAGYYCVDNLPPELLLSFVA
                     LEQQASAPRVAIAMDVRSATSLPLVPQQLRELRRQGVLVRPLFLDATTETLVRRFSET
                     RRRHPLSGNRPGAQASDLAHDRQHALVDAIEIERELLADLREQAHVIDTSVIRPSQLQ
                     AHVKSLLSAPISQLTLVFESFAFKRGIPVDADFVFDVRMLPNPHYEPALRDLTGRDEA
                     VIAFLKHHADVEQMYSHIEHFLNHWLEPLARDHRSYVTVAIGCTGGQHRSVFLVEQLA
                     ASFGKRWITLKRHRELDPGS"
     gene            complement(8921..10585)
                     /gene="recN"
                     /locus_tag="VGA59_01280"
     CDS             complement(8921..10585)
                     /gene="recN"
                     /locus_tag="VGA59_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX9718337.1"
                     /translation="MPLKRIALRDFVIVRELDLDLESGFSVLTGETGAGKSILIDALQ
                     LALGARAEAAVVREGAARTDISAEFDLPPALGPWLEEAGFEAGDSLLLRRSIDNQGKS
                     RAWINGSPATATQLRELADQLVDIHGQHAWQSLTRADAVRGLLDGYAGVTTQPLQQLW
                     QRWRAAQKTLADARAAQDSLQRERERLAWQIGEVDKLAPGADEWDELNTSHTRLANAQ
                     SLLEAAQGALEALQAEEGGATGRLAQAQALLQGQEHVEPVFSELAQVLASSLAQAEDA
                     AHSLHAYLRKTELDPQRLSELDERMSLWMSLARRYKRTPPELPGLLASWKQELARLDA
                     AADLAVLEAAEKKEADAYMAEARALSKARTRAAPQLAKAVTHAMQGLGMQGGRFEVAV
                     HKAGQPAAGGLEEVAFLVAGHAGGTPRPVGKVASGGELSRIALAIAVTTSRLGTAQTL
                     IFDEVDAGIGGAVAETVGRLMKQLGRDRQVLAVTHLPQVAACADHHLLVARQSDALGP
                     SSTVAAVQGEHRVGEVARMLGGERLSGTTLAHAKEMLSGSGAGTPI"
     gene            complement(10616..11509)
                     /locus_tag="VGA59_01285"
     CDS             complement(10616..11509)
                     /locus_tag="VGA59_01285"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX9718338.1"
                     /translation="MKSQFRQVALIGKYHAAVSGSAAGSTRAALDDIAHFLHSQGCEV
                     VFERDTASSTGITGYTTLEVPAIGNQCDLILVVGGDGTMLGIGRQLARHGVPLIGINH
                     GRLGFITDIPLDHYRATLEPMLRGEYEEDHRSLMHARVMRDGHCVFDALAMNDVVVNR
                     GATSGMVELRVEVDGHFVANQRCDGLIVASPTGSTAYSLSAGGPLLHPSTPGWVLVPI
                     APHTLSNRPIVLPDSSEITIEVVSGRDASANFDMQSLASLLHGDRISVRRSQHRVRFL
                     HPRGWTYFDTLRKKLHWYEGN"
     gene            11550..12551
                     /gene="hrcA"
                     /locus_tag="VGA59_01290"
     CDS             11550..12551
                     /gene="hrcA"
                     /locus_tag="VGA59_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX9718339.1"
                     /translation="MLDDRAKLLLKALVERYIAEGQPVGSRTLSRASGLELSPATIRN
                     VMSDLEDLGLIASPHTSAGRIPTARGYRLFVDTMLTVQREELATPSLAADQPQKVIAN
                     AAHLLSNLSQFVGVVMAPRRTSVFRHIEFLRLSERRYLVIIVSPDGDVQNRVVFTEVD
                     YTQTELIEAANFLNAHYAGMAIEQVRERLKVEVESLRSEIAALMQTAVQASSEAMSEQ
                     DEVVISGERNLLAVSDFSSDMSHLRRAFELFEQKTQLMRLLDISSQAAGVRIFIGGES
                     QVVPFEELSIVSAPYKVDGQIVGTVGVIGPTRMPYDRMIQIVDITSKLVSNALSHHK"
     gene            12626..12702
                     /locus_tag="VGA59_01295"
     tRNA            12626..12702
                     /locus_tag="VGA59_01295"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12660..12662,aa:Ile,seq:cat)
BASE COUNT         2061 a         4402 c         4298 g         2087 t
ORIGIN      
        1 gtatcgagct gcttggccag cgcccgggcc cgcaccacgc cgcccacgtc gggcgacacg
       61 acgatcaggt cgtcgtagtt cttgtggcgc aggtcgccca gcagcaccgg cgaagcataa
      121 atgttgtcca ccggaatgtc gaaaaagccc tggatctggt cggcgtgcag gtccatggtc
      181 agcacgcggg ccacgcccac cgtctgcagc aggttggcca ccaccttggc cgagatcggc
      241 acgcgggtgg aacgcgggcg ccgatcctgg cgtgcgtagc cgaaatacgg aatcacggcg
      301 ctgatccgct cggccgaggc gcgcttgagc gcgtcgacca tgatcagcag ttccatcagg
      361 ttctcattgg tcggcgcgca ggtggactgc accacgaaga cgtcgcgcgc ccgcacgttg
      421 gtgcttatct cgacggtgac ttcgccatcg gaaaagcggc ctacgtgggc cgcgcccagc
      481 gaaataccca ggtggccggc aatctccgcc gcgaggtcgg gattggcgtt gcctgtgaac
      541 accatgaaat cgggatgtgg aacaacctgc atgaccggac ccagcgatta ataaacaagt
      601 cgtggggtca gaacccaatg tttcgggccg ttaagccata aagttcaggc caaaaattgg
      661 gttctgaccc caattaaatg gcaggggagg aaggactcga accctcgcat gctggaatca
      721 aaatccagtg ccttaaccaa cttggcgact cccctacacg ggttgacggc tgcatttcag
      781 ccgcccaccg atgaatcgtc gctgggtgcc catcccacca gaggatgagc ctccaaattc
      841 ccgcattccc tcacttgcca gccggccgga gcggcctgca agctccggcc ctgcggcagc
      901 tgcgaaaaca cgccactccc cgagcctgtc atgcgccccg ccaatccctg agagcccagc
      961 cattgaaggg cctgggtgac gccggggcaa agcttttgcg cgaccggctg caagtcgtta
     1021 cggccaaatc cgaagggatt ttccatgaaa tcctccaaag agggtcttcc ggattcgccg
     1081 tattgcttcg cagcaaagcc tgaaattata gcagtgtcat cgctgcgatt gagccgcagg
     1141 tcgttgaaga caagccccgt cgacagtccc tgcggcggct tcgccacggc aaaccgggcc
     1201 gccggcagca cgacgggctt cagccgctcg ccgattccct cgacccaggc attgcggccg
     1261 tgcagaaaaa acggaacgtc ggcacccagc gccagcccga tccgggccag ctcgggcacc
     1321 gtcaggccca ggcgccacag gcgattgagc gccagcaggc aggtggccgc gtcggaggat
     1381 ccgccgccca gtccggcctg ggccggcacg cgcttgtcga tggcgatgtg cgcccccgcg
     1441 gtcgcgcccg aggcggcctg caagctgcgc gcggcgcgca gcacgaggtc gtcggccggc
     1501 aagggctcgc cgaggtcctc gcggctgagc gcacggccgg tgcgcagttc gaaattgagt
     1561 gtgtccgacc agtcgatgag catgaacacg gactgcaaca gatgcagtcc atccggcctg
     1621 cggccggtga catgcaggaa caggttcagc ttggcgggcg cgggaacgtc gtacagcgcc
     1681 ttcatgcgcg gggccctaac gctccagcac cacccgcagg tcggcccgcg gcggcggctg
     1741 aagccgctgc gccctcagcc gcccttggcc cagcagcgag aggtcggcct tccatccggg
     1801 aaccggcgta tcgataccgc gcagccagtc gaacagcgcg gcgaccggga tggcggcgcc
     1861 ggtggcatgc gccaccagcg actcgaccga ctcgaactcg cgcgcctgtc cgttggacag
     1921 cagcctggcc gaccccgggg cccaggccag cacggccatc gtggcgccaa gcgggttata
     1981 cagggccagc tcgcccgacg tggcggtgcc cttgagttcg aagcccgccg aaaacgactg
     2041 gggctggttt tcttccacct ccagcgccag ccgtccgctc catggcccgg acagcgcatc
     2101 ggtgggaccg gctgtcctgg gagggcttgc gcatcccgcc gcgacagcga tggcgagcgc
     2161 gatcgcccaa cgggcccagg gcctgtgcat gctcatagct tgacgcgcag gcgcttgagc
     2221 gtttcctgca gtgtttcgct ttcgctgttg agcaagaggc cttccttcca gatggcctgc
     2281 gcgcgctcgc gctggcccag gctccagagc acttcaccga gatgggccgc gatctcggcg
     2341 tccggacgca tcttgtaagc ggcggacagg atgcgcagtg cctcgtcccg gttgcccatg
     2401 cggaactcga cccaggccag gctgtcgctg atgaacgggt cctcgggggc ataggtcaac
     2461 gccttctgga tgagctcctt ggcttcgggc aggcgcatgt tgcgatcggc cagcgaatag
     2521 cccagtgcgt tgtaggcgtg gtggtaatcc ggcttgttgg cgatcacccc ccgcagcagc
     2581 cgttccatgt cggccaggtt gcccatcttc tcggccacca tggcctgctc gtagatcagg
     2641 tccgggtcca gttccagggg cgctttggcc gtggcctgcg ccagcaggtc atacgcggcc
     2701 ttccactgct tgtgctcgcg cagcaactgg acctcggcgg tgatcttcat gcgcgtgtcg
     2761 tcggcgttgc gctccggcaa tgagcgcagc aactggcgcg cctgctccag cttgccctgc
     2821 cgcgccagga tcgaagcccg gcggttctgc gccgccacga gatcctgcgg gttttcgatc
     2881 ttgtccagcc aggcgctcgc cagcgggtag tccttgcgtt tctcggcgat gcgcgccagc
     2941 gacaggtagg cctgcgcctg gccgcggctg cgttcctcgc ccgtgcgctg gctttgcgcc
     3001 agctcgaggt agcgcttgag cgaggcctcg gctgcggcat cctggttgtc ctgcagctgc
     3061 agcgtgccct gcaccagcca ggcctccggg taatcgggct tctgcgccgt caccagctgc
     3121 agctgctggg acgcctcggc atagcgctgc gcgtcgagca gcgcgcgtgc atagcccatg
     3181 cgcaattccg gaagcggctg gcctgccatg tagcggcgca cgatggcttc ggcctggggc
     3241 agcttgggat ccatcagttc catggccagc agcgcgggag cttcggcctg cgcattggcg
     3301 gcctgcccgc gcgtcgccgc atccaccgcg ccggtgttgt cgccggccaa cagccgcatg
     3361 cgtccgaccg cggtccacgc cgcggcggcc gtcgccggct gcgccaggta ctcgcccagg
     3421 gccttttcga ccacctcggc ggcaagtttc ttgtcgctca cccgcgagta gctgcgcggg
     3481 atggcggcca gcaccgactc gcggtcctgc ggcccggcca gggcaagttc ggtctgcaac
     3541 ggctcggcgc tgtcggcgat gcggttgagc gccaccagga tctgcaacac gtagcggttg
     3601 gcctcgcgcg actcggggaa ggcctgtttc caggcccggg ccgcctgcag cgccgcgtcg
     3661 cccgagcggg actggaacgc aacctccacc gcgcgctggt agagcgccgg gtcattggta
     3721 cggcgggcgg catccaggat cagcgaatag ccggccgcgg gttccacccc ccgggcattg
     3781 atctcgccca gcagcagttg gtagaacagc tcggcgtcga gcggcgtggt ggccgcggcg
     3841 ccgtccgggt cgccggcggc gggcaactgc gcgcacgcgg cctgggccaa tgccagcagg
     3901 agcagcgcgc cggccgcaag ggaaaatcgc ttcatcgaac cataataatc caagccccgc
     3961 cgtcattgcg tcccatgccc gaactgcccg aagtcgaggt gacccgcctg agtcttgccg
     4021 accgtatcgc cggcgcacgc atcgaagcgg tgcgcgtggg caagccgctg cgctggccgc
     4081 tgggactgca ggccgaccgg ctggtcgggc ggacggtgcg cggcgtgcgg cggcgcggca
     4141 agtacctgtt gatggacctg gacgaaggcc tgctgctgtt gcacctgggc atgtcgggca
     4201 gcctgagctt cgcgccgcgc ccgccgctcg ccggtgcgca cgaccacttc gacctggtga
     4261 ccagccgcgg catcctgcgc ctgaacgatc cgcgccgctt cggcgccgtc gtctatgccg
     4321 ccggcgagaa ctcgcccgag gccagcaagc tcctgggccg cctgggtgtc gagcccttga
     4381 gcgaagcctt cggcctggac ctctttcacg ccggcctcaa gcgccgccat gcgccggtga
     4441 agcaggtgct gctggcgggc gacctcgtgg tcggcgtcgg caacatctat gcgtcggaag
     4501 cgctgttcct ggcgggcatc cggcccacgc tgcgggcctc gcgcatcagc cggccgcgtg
     4561 cggaacggct gcgcgccgcc gttcgggagg tgctggcgcg cgccgtcgcg gtgggcggca
     4621 gcacgctgcg cgatttctcc aacgcgcacg gcgaggccgg ctatttccag ctcgaagcga
     4681 tggtgtacga ccgcgccggc gagccgtgca ggatttgcgg aacccccgtg aaaagtatcc
     4741 gccagggcca gcgcgcgtcg tatttctgcc ccaattgcca gaaatcctga aggtcctgtt
     4801 gggctcggat gctatattgg caaacactcc ggggccacag tgggtacttc cttcaacgac
     4861 cagttcgacc agcacggcgt atggcggcgc gagttcgccc tgcgcctgaa gctgctgtcc
     4921 gaatggctga aggaccacga actgctcgat gcggccgtgg aggagcgctt gcggcgcctg
     4981 gagacgcaga tccgttccga aaaggtcatg gtggccttcg tcgccgaatt ctcccgcggc
     5041 aagtcggagt tgatcaacgc catcttcttt gccggctaca agcgccgcat catgcccgcc
     5101 agcgccggcc gcaccacgat gtgcccgacc gagctgggct acgacgtggg cgtgccgccg
     5161 tgcctgcgcc tgttgccgat cgaaacccgg ctgcagcccc agccgctgat ggaatggcgc
     5221 atggtccccg agaagtggat ccgcgtcgtt ctcgacgtca acgatcccgc gcagctcgcc
     5281 aaggccttcg aaaaggtcgc cgaagtgcgg catgtgacca aggacgaggc caaggcgctg
     5341 ggcttctggc acgacgacca gcccgacgac aacccgctcg tgggcgcgga cggcaaggtg
     5401 gaagtgcccc ggtggcgcca tgccctgatc aacatcgccc acccgctgct caagcagggc
     5461 ctggtgatcc tggacacgcc gggcttgaac gcgatcggcg ccgagcccga actgaccgtg
     5521 aacctgatcc cgcaggcgca cgcggtcgtg ttcatcctgg cggccgatac cggcgtcacc
     5581 aagtccgatc tcgccatctg gcgcgagcac ctgatcaccg aggccgacaa catcgagtcg
     5641 cgcctggtcg ttctgaacaa gatcgacacc atgtgggatt cgctgagcac gccggcccag
     5701 gtgcaggccg agatcgaccg gcagcgcgcc acctcggccg aaatcctggg cctgccgctg
     5761 gagcaggtga ttcccgtgtc ggcgcagaag ggcctggtgg ccaaggtcac cggcgatacc
     5821 gctttgctgc aggccagcga gctgcccgtg ctcgagcgcg cgctgggcca gggcgtgatg
     5881 ggccagcgcc agcggatcct gcgctccgcg gtggaaagcg gcatcggcga gctgcgggtg
     5941 gaagccgccc gcgcgatcgg cattcgccgg cgcgacctgg ccgagcagat ggtcgaactc
     6001 aggggcctgc gcggcaagaa catcgccgtc atcaggcata tgcgctcgcg catcgaacag
     6061 gagcagcggg aattcaatgt gagcggcgcg cgcattcatg ccgtccgctt cgtgcacctg
     6121 aaactgctgc acgaagtctt cgtccagctg ggcaccgcca cgctcaaggt cgaaatggcc
     6181 gaacttacga cggccctgcg ccagcccggg atcaagctgg gcgtgaggaa agcctatgcg
     6241 cagacattcg accgcctgcg ggcggtcctg cgcaaggccc agaccaccag cgccgagatc
     6301 cagtccatgc tgaccggaac cttccgccag ctcaatgccg aatacggctt ctcgctgcag
     6361 gccccgcgcg agccggaact ggaccgctac gtgcgcgacc tggacctggt ggagcgcagc
     6421 cacaaccagt acctgggcgt gaccaacgtg ttccggctgg cacagcccga gttcgcggac
     6481 cggctggtgc gcgccctcgc cacgcgactg cgcgtggtgt acgaatcggc gctgggcgaa
     6541 gtggagctgt ggaacaagtc ggcggccgcc cagctcgatg cgcagctgcg cgagcggcgc
     6601 cgcactttca cgcgccgcct ggaagcgatc gagcggatcc agcaggcggc cagcggcctg
     6661 gacgatcgca tcggtgagat cgggcagcag gagcgcgcgc tcgacgatct caacgccaag
     6721 ctcaccgagc tgacggatca cctggtcaac gctcccggca gcgccggcca ccagccggcc
     6781 gagcccacgc tgcaaagggc atgagcttcg ccggccgcat cgtgcgctgg cagcgcacgc
     6841 acggccgcaa ccacctgccg tggcagaaca cgcgcgacgc ctaccgggtc tggctgtccg
     6901 agatcatgct gcagcaaacg caggtggcga cggttctcga ctactacgcc cgcttcatcg
     6961 cccgctttcc ggatgtgagg gcgctggccg ccgccgaagt ggacgaagtg ctcgccctgt
     7021 ggagcgggct gggctattac agccgtgcgc gcaacctgca tcgctgtgcc cgcgaagtcg
     7081 tggcgctgca tggcggcgag tttccgggca atgcgcaggc gctgcggacc ctgccgggca
     7141 tcggccgctc caccgccgcg gccatcgcat cgttctgctt cggcgaacgc gtggccatcc
     7201 tggacggcaa tgtcaggcgc gtgctcaccc gcgtcctggg tttcggcgcc gaccttgcca
     7261 gcgcaagcca cgagcgccag ctatgggaac gggcgcaagc gctcctgccg cagcgctcgg
     7321 ccaggcaaac catgccgcgc tacacccagg gcctgatgga cctgggcgcc accatctgcc
     7381 tggcgcgcaa gccctcctgc ctgctctgcc cggtgcgcga gctgtgcgtg gcccaccgcg
     7441 aaggcgcacc ggaaaactac cccgtgaaga cccgcaagct caagcgcggc gcgcaatctc
     7501 tgtggctgct gcgggcacag gccggcgatg gttccgtgtg gctcgagaaa agacccgccc
     7561 aaggcatctg gggcggcctg cattgcctgc ccgtgtttga aagccgcgat gcgctgcagg
     7621 cggcggtgcc ggcggccgca agggtccgtc tgcaggacga gacgccgttt ctccacgtgc
     7681 tcacgcacaa ggatctgcac ctgcatccgg tcggggcggc gatttccagg acttcgatgc
     7741 gtggcgcgca agggggctgg ttcggcagtc acgagtgggc gcagctgggc ttgcccgcgc
     7801 cgatccgccg gttgcttgcc cgcggctaag aagtctgcgc ggcagaagcc ggtattaccc
     7861 cctctccctc tgggagaggg ctggggtgag ggcatgcgag cccggcatga cagcagccgg
     7921 ggaggcagct gctatgcgat atcccgaacg gcgccgccca gcaagcgcgt attgagcgtg
     7981 ccgccggaaa tcctcatttc gcccatccgt gctcccggct gcccccctca agagcccgga
     8041 tccagctcgc gatgccgctt gagcgtgatc cagcgcttgc cgaacgaggc cgcgagctgc
     8101 tccaccagga acaccgagcg gtgctgtcca cccgtgcatc cgatcgccac cgtcacgtag
     8161 ctgcggtggt cccgcgccag cggctcgagc cagtgattca ggaaatgctc gatgtggctg
     8221 tacatctgct ccacgtcggc atggtgcttg aggaaggcga tgacggcttc gtcgcggccg
     8281 gtcaggtcgc gcagcgccgg ttcgtaatgg gggttcggca gcatgcgcac gtcgaacacg
     8341 aaatcggcgt cgaccggaat gccgcgcttg aaggcgaacg attcgaacac cagcgtgagc
     8401 tggctgatcg gcgccgacag cagcgacttg acgtgggcct gcaactgcga tggccggatg
     8461 acgctggtgt cgatcacgtg ggcctgctcg cgcaggtccg ccagcagctc gcgctcgatt
     8521 tcgatcgcgt ccaccagcgc atgctggcgg tcatgcgcca ggtcgctggc ctgggccccg
     8581 ggccggttgc ccgacagcgg atggcggcgc cgggtctcgg aaaaccgccg caccagcgtt
     8641 tcggtggtgg cgtccaggaa cagcgggcgc accaggacgc cctgccgccg cagctcgcgc
     8701 agctgctgcg gcaccagcgg cagtgacgtg gcgctgcgca cgtccatggc gatcgccacg
     8761 cgcggcgcgc tggcctgctg ttccaaggcg acgaatgaca gcagcagctc gggcggcagg
     8821 ttgtccacgc agtaatagcc ggcgtcctcc agcgcgcgca atgccacgga cttgccggag
     8881 cccgacatgc cggtgatcag gacgagctcg agtgtcatgg ctatatgggt gtcccggcgc
     8941 cggagcccga gagcatctcc ttggcgtgcg ccagcgtcgt ccccgacagg cgttcgccgc
     9001 ccagcatgcg tgcgacctcg ccgacgcggt gttcgccctg cacggcggcc acggtactgg
     9061 acggccccag ggcgtcgctt tgcctggcca ccagcaggtg gtggtcggcg caggccgcga
     9121 cctgcggcag gtgggtcacg gcgagcacct gccggtcgcg ccccagctgc ttcatgagcc
     9181 ggccgacggt ttcggccacc gctccgccga taccggcatc cacctcatcg aagatcagcg
     9241 tctgcgccgt gccgagccgg ctggtcgtca cggcgatcgc cagggcgatg cgcgacagct
     9301 cgccgccgga agccaccttg cccaccggcc ggggcgtgcc gcccgcgtgg ccggccacca
     9361 ggaaagccac ttcttccagg ccgccggccg ccggctgtcc ggctttgtgc acggccactt
     9421 cgaagcgccc gccctgcatg cccaaccctt gcatcgcgtg cgtgacggcc ttggccagct
     9481 gcggtgcggc ccgggtcctg gccttcgaca gcgcccgggc ctcggccatg taggcatcgg
     9541 cctccttctt ttcggcggct tccagcacgg ccaggtcggc ggcggcgtcc agcctggcga
     9601 gttcctgctt ccaggacgcc agcagaccgg gcagctcggg cggcgtgcgc ttgtaacggc
     9661 gcgcgagcga catccacagc gacatccgct cgtccagttc cgacaggcgc tgcgggtcca
     9721 gttcggtctt gcgcaggtag gcatgcagcg aatgcgccgc gtcctcggcc tgggccaggc
     9781 tggaggccag cacctgcgcg agctccgaga acaccggctc gacatgttcc tggccttgca
     9841 gcagggcctg cgcctgcgcg agccgcccgg tggcgccgcc ttcttcggct tgcagcgcct
     9901 cgagcgcgcc ttgcgccgct tccagcaggg actgggcgtt ggccaggcgg gtatggctgg
     9961 tattgagttc gtcccattcg tcggcgcccg gcgcgagttt gtccacttcg ccgatctgcc
    10021 aggccagccg ctcgcgttcg cgctgcagcg aatcctgcgc cgcccgggcg tcggccagcg
    10081 tcttttgcgc cgcccgccag cgttgccaga gctgttgcag cggctgggtg gtcacgcccg
    10141 catagccgtc cagcaagccg cgcacggcat cggcccgcgt caggctttgc caggcgtgct
    10201 gcccgtggat gtccaccagc tggtccgcca attcgcgcag ttgcgtggcg gtggccgggc
    10261 tgccgttgat ccaggcgcgg ctcttgccct ggttgtcgat gctgcggcgc agcaacaagc
    10321 tgtccccggc ctcgaagccc gcttcttcca gccacggccc cagcgccggc ggcaggtcga
    10381 actcggcgct gatgtccgtg cgcgccgcgc cctcgcgcac cacggcggct tcggcgcgcg
    10441 cgcccagtgc cagctgcagc gcgtcgatga ggatggactt gccggcgccg gtttcgccgg
    10501 tcagcacgct gaagccgctt tccaggtcga ggtcgagctc gcgcacgatg acgaagtcgc
    10561 gcagggcgat gcgtttcagc ggcatgtcgg cctccggccg ggcgctgctc ccatttcagt
    10621 ttccctcata ccagtgcaat ttcttgcgca gcgtgtcgaa ataggtccag ccgcgcgggt
    10681 gcaggaagcg cacccggtgc tgcgaccgcc gcacgctgat gcggtcgccg tgcagcagcg
    10741 aggcgagcga ctgcatgtcg aaattggcgc tggcgtcgcg ccccgagacc acctcgatgg
    10801 tgatttccga cgagtccggc aggacgatcg gccggttgga cagcgtgtgc ggcgcgatcg
    10861 gcaccagcac ccagcccggc gtggacggat gcagcaacgg cccgccggcc gacagcgagt
    10921 aggcggtgga accggtgggc gacgcgacga tcaggccgtc gcagcgctgg ttggccacga
    10981 aatgcccatc gacttccacc cgcagctcga ccatgcccga ggtggcgccg cggttgacca
    11041 cgacgtcgtt catcgccagc gcgtcgaaca cgcaatggcc gtcgcgcatg acccgggcat
    11101 gcatcaggct gcgatggtcc tcctcgtact cgccgcgcag catcggctcc agcgtggcgc
    11161 ggtaatggtc caggggaatg tcggtgatga agcccagtcg cccgtggttg atgccgatca
    11221 ggggcacgcc gtgacgcgcc agctgccggc cgatgcccag catggtgccg tcgccgccca
    11281 ccaccaggat caggtcgcac tggttgccga tggccggaac ctccagggtg gtgtaccctg
    11341 taattcccgt actggatgcc gtatcgcgct cgaagacgac ttcgcagcct tgggagtgca
    11401 ggaagtgagc aatatcgtcc aaagcggctc gggtggagcc cgccgccgat cccgagaccg
    11461 cggcatggta cttgccgata agggccacct ggcggaattg ggatttcatt ggcaaattac
    11521 atcattaaat ttagataatc cggctaatca tgctcgatga ccgtgccaag ttgttgttga
    11581 aagccctggt cgagcgctac atcgccgagg ggcagccggt gggctcgcga accctgtcgc
    11641 gggcctcggg cctggagctg tcccccgcca ccatccgcaa cgtgatgtcc gacctggagg
    11701 acctgggcct gatcgccagt ccgcacacgt cggccgggcg cattcccacg gcgcgcggct
    11761 accggctgtt cgtcgacacc atgctcacgg tgcagcgcga agaactcgcc acgcccagcc
    11821 tggcggcgga ccagccgcag aaggtgattg ccaacgcggc gcacctgctg tccaacctgt
    11881 cgcagttcgt gggcgtggtg atggcgccgc gccgcacctc ggtgttccgg cacatcgaat
    11941 tcctgcgcct gtcggagcgg cgctacctgg tcatcatcgt ctcgcccgac ggcgacgtgc
    12001 agaaccgcgt ggtgttcacc gaagtggact acacgcagac cgaactcata gaggccgcca
    12061 atttcctgaa cgcgcactac gccggcatgg cgatcgagca ggtgcgcgag cgcctgaaag
    12121 tcgaggtgga gtcgctgcgc agcgaaatcg cggcgctgat gcagaccgcc gtgcaggcca
    12181 gctccgaggc catgagcgaa caggacgagg tggtgatctc cggcgagcgc aacctgctgg
    12241 ccgtgagcga cttttccagc gacatgagcc acctgcgccg cgccttcgag ctgttcgagc
    12301 agaagacgca gctgatgcgc ctgctggaca tatccagcca ggccgcgggc gtgcgcatct
    12361 tcatcggcgg cgagagccag gtcgtgccgt tcgaggagtt gtcgatcgtc agcgccccgt
    12421 acaaggtgga cgggcagatt gtcggcaccg tcggcgtgat cggcccgacc cgcatgccct
    12481 atgaccggat gatccagatc gtggacatca cgtccaagct ggtcagcaat gcgctgagcc
    12541 accacaagtg aatgccgcgt cgcggcagtc atcccggcgc gcaggccggg atcgatccat
    12601 atctccctac aatcgcagtt cggtcggggc gttagctcag ttggttagag cagaggactc
    12661 ataatccttt ggtcgttggt tcaaatccaa cacgccctac cagtgaaatc tttttcgatg
    12721 tagtcgtttt tcagttcacc ccgtttttca ccccgtatgg gcaagatcag caaggtcgcg
    12781 aatccggggc taccccagaa acggaaccct ctccccggcg cgctgcctaa acgccaagat
    12841 catcgctc
//
LOCUS       DASWIY010000034         3843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2606765, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000034 DASWIY010000000
VERSION     DASWIY010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2606765"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..893
                     /locus_tag="VGA59_01300"
     CDS             69..893
                     /locus_tag="VGA59_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9718340.1"
                     /translation="MNSAIQSAHRAAPQPFGEHLRHWRRHRRLSQLDLAQEAEISTRH
                     LSFVETGRSLPSREMVLRLAQRLDVPLRERNTLLVAAGYAPMYRERPLDDPALSAARQ
                     AVELILKCHEPYPALAVDRHWNLVAANSMLPHLLAGADPSLLQGQVNVLRLSLNPRGL
                     ASRIVNLAQWRSHLFERLRQQIHATGDARLAELLEELHGYPVPDGTDQLQLEGEHLGV
                     VMPFRFRSSFGVLSFISTTTIFGTPVDVTLQELAMETFFPADAVTGDVLRKLMSPA"
     gene            944..3154
                     /locus_tag="VGA59_01305"
     CDS             944..3154
                     /locus_tag="VGA59_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX9718341.1"
                     /translation="MSETIHHRICPLCEACCGLELKVSAGKVIGIRGHDADVFSAGYI
                     CPKGVALKDLHEDPDRLRTPLIKRNGRFVEASWDEAFAEIERRLPPVVAAHGRDAVAL
                     SLGNPAAHKMALLLYGPRLRKALGTRNLFSASTLDQMPKHLSSGLMFGHLFSIPVPDI
                     VRCDYLLMLGANPMASNGSLWTVPDFRGKAKAMRSRGGRLVVIDPRRTETAALADEHH
                     FIRPGADVFLLLGMVHALFDENLVRLGRLAPYLNGVDAVQSAVKPFAPEAVAERCGIE
                     PPAIRRLARELAAAPRAAVYGRIGTCTQEYGTLVSWLVDLLNALTGHLDEPGGAMFPK
                     AAAFARNTAGTPGTGSGVATGRYKSRVSGAPEVFGELPMTCLAEEIETPGTGRVRALV
                     TVASNPVLSSPNGPRLAAALDSLDFMVSMDIYLNETSRHADVILPAPSPLEDSHYDVA
                     FPQLSWRNHARYSAAVFDLPPGQLPEWATLLKLAAIVEGRGANADPAQLDDERFALDS
                     AKLFGEHAARVIEATAHLEGPERLLDVALRTGPYGDSFGRQPAGLNLAKVRAAPAGID
                     LGELAPRIPELLRTPSGKVELAPPALLADLQRPAQDLARRAPPMVIVGRREVRSNNSW
                     MHNLPLLAKGPERCTALVHPADAQRLGLADGAMARISSRAGTIEARVQVNGDMMPGVV
                     SLPHGWGHNLPGSRLGLAAQRPGVNLNQLMDEGWRDPFSGNAVLNGIPVELEPV"
     gene            complement(3151..>3843)
                     /locus_tag="VGA59_01310"
     CDS             complement(3151..>3843)
                     /locus_tag="VGA59_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HEX9718342.1"
                     /translation="WERLRNIEGAAQRVQEDTMRFARLMEGLGVDFMRSVMTLFAFLP
                     ILWTLSAKVTELPWLGPVSHSLVWVAIGFALAGTVLMAVVGIKLPGLEFQNQRVEAAY
                     RKELVYGEDDAGRAAPPVAKTLFADVRRNYFRLFFHYLYFDVVKWSYLQFGVLVPYIA
                     LGPTLVTGLITLGVMQQIVRAFGRVEQSFQYLVLSWTTVVELISVFKRLRAFEFQIRQ
                     GAGTPPLAAESA"
BASE COUNT          638 a         1324 c         1277 g          604 t
ORIGIN      
        1 cggtcgatta cgcgcaaggt aatgggagtt cgggctattt gcctgactca ttggactagc
       61 atggcgccat gaacagcgcg atccagtccg cccatcgagc cgccccgcag cctttcggcg
      121 agcacctgcg ccactggcgg cggcaccgtc ggctcagcca gctcgacctg gcccaggaag
      181 ccgagatctc gacccgccat ctcagctttg tcgaaaccgg ccgctccctg cccagccgcg
      241 agatggtgtt gcggctcgcg cagcggctgg acgtgccctt gcgggagcgc aacaccctgc
      301 tggtggccgc cggctacgcc cccatgtatc gagaacgccc gctcgacgat ccagccctgt
      361 ccgccgcccg ccaggcggtc gaactcatcc tcaagtgtca tgagccctat cccgcgctcg
      421 cggtggaccg gcactggaat ctggtggcgg ccaacagcat gctgccgcat ctgctggccg
      481 gcgccgaccc ctcgctcctg cagggacagg tcaacgtcct gcggctgagc ctgaatccgc
      541 gggggctggc gtcccggatc gtcaacctgg cccagtggcg cagccacctg ttcgagcgtt
      601 tgcgccagca gatccacgcg accggcgacg cccggctggc ggaactgctg gaggaattgc
      661 acggctaccc ggtgccggac ggaacggacc agctgcagtt ggagggcgag catctcgggg
      721 tcgtgatgcc gttccggttc cgcagcagct tcggcgtgct gagcttcatc agcacgacca
      781 cgatcttcgg cacgccggtg gacgtgacgc tgcaggagct cgcgatggag accttctttc
      841 cggctgacgc agtgacgggc gacgtgctgc gaaagctgat gtcgcccgcg tgatcgcctg
      901 gccgatgctt tccgtgcagg atggaccatc ccttggagac tccatgagcg aaaccattca
      961 tcaccggatc tgtcccctgt gcgaagcctg ctgcggcctt gagctgaaag taagcgcggg
     1021 aaaggtcatc ggcatccgcg gtcatgacgc ggacgtcttc agcgccggct acatctgccc
     1081 caagggcgtg gcgctcaagg acctgcacga agacccggat cgcctgcgca caccgctgat
     1141 caaacgcaac ggccgattcg tggaagcaag ctgggacgaa gccttcgccg agatcgagcg
     1201 ccgcctgcct ccggtcgtcg ccgcgcatgg ccgcgacgcc gtcgccttgt cgctgggcaa
     1261 tccggccgcg cacaagatgg cgctgctgct gtatggtccc cggttgagga aagcgctggg
     1321 cacccgcaac ctgttctccg cctcgaccct ggaccagatg cccaagcacc tgtccagcgg
     1381 gctgatgttc ggtcacctgt tcagcattcc ggtgcccgac atcgtgcgct gcgactacct
     1441 gttgatgctg ggcgccaacc cgatggccag caacggcagc ctgtggacgg ttccagattt
     1501 tcgcggcaag gcgaaggcca tgcgctcgcg tggcggcagg ctggttgtga tcgatccgag
     1561 gcggaccgaa acggcggcgc tggcggacga acaccatttc atccgtccgg gcgcggatgt
     1621 gttcctgctg ctcggcatgg ttcacgcgct gttcgacgag aacctggtgc ggctcggtcg
     1681 cctggcgccg tacctgaatg gcgtggatgc ggtgcagtcc gcggtcaagc cgtttgcgcc
     1741 cgaagcggtc gccgagcgct gcggtatcga gccaccggcg atcaggcgcc tggcacgcga
     1801 gctggcggcc gccccgcgtg cggcagtgta tggccgcatc ggcacctgca cccaggaata
     1861 cggcacgctg gtgagctggc tggtggacct gctgaacgcg ctcacggggc acctggacga
     1921 gcccggcggc gcgatgtttc ccaaggccgc ggcctttgcc cgcaacacgg cgggcacgcc
     1981 aggcaccggc agcggcgtcg ccaccggccg ctacaagagc cgcgtcagcg gcgcgccgga
     2041 agtcttcggc gaactgccca tgacctgcct ggccgaagag atcgagacgc cgggcaccgg
     2101 ccgggtgcgc gcgctggtca ctgtggccag caatccggtc ctctcgtccc cgaacggccc
     2161 gcgcctggcc gcggcgctgg attcactgga cttcatggtc agcatggaca tctacctgaa
     2221 cgaaacctcg cgccatgccg acgtgatcct gccggcgccg tcgccgctcg aggacagcca
     2281 ctacgacgtc gctttcccgc agctgtcgtg gcgcaatcac gcacgctaca gcgcggcggt
     2341 gttcgacctg ccgccgggcc agctgccgga atgggcaact ctgctcaaac tcgccgccat
     2401 cgtcgagggc cgcggcgcga acgcggaccc cgcgcagctc gacgacgaac ggttcgccct
     2461 cgattcggca aagttgtttg gcgaacacgc cgcccgggtg atcgaggcga ccgcgcacct
     2521 cgaagggccg gagcgcctgc tggatgtggc cctgcgcacc gggccatacg gcgacagctt
     2581 cggccgccaa ccggcgggcc tgaacctggc caaggtgcgg gcggcgccgg caggcatcga
     2641 cctgggcgag ctcgcgccgc gcattccgga gttgctgcgc acgccatccg gaaaggtcga
     2701 actggccccg cccgcgctgc tggcggacct gcagcggccg gcgcaggatc tggcgcggcg
     2761 ggcgccgccg atggtcatcg tcgggcggcg cgaggtgcgc tccaacaaca gctggatgca
     2821 caacctgccg ctgctggcga agggcccgga gcgttgtacc gccctcgtcc atccggcgga
     2881 tgcgcagcgc ctggggctgg ccgacggcgc gatggcgcgc atcagcagcc gtgccggcac
     2941 catcgaggcg cgagtccagg tgaacggcga catgatgccg ggcgtggtga gcctgccgca
     3001 cggctggggc cataacctgc ccggcagccg ccttggcctg gcggcgcagc ggcccggcgt
     3061 caacctcaac caattgatgg atgagggttg gcgcgatccc ttctcgggca atgcggtgtt
     3121 gaacggcatt cccgtcgagc tggagccggt ctaggcactt tcggccgcga gcggcggcgt
     3181 cccggcgccc tgacggatct gaaactcgaa ggcgcgcagc cgtttgaaca ccgagatcag
     3241 ctccaccacc gtggtccagc tgagcaccag gtactggaat gactgctcga cgcgaccgaa
     3301 cgcgcgaacg atctgctgca tcacgcccag cgtgatcagg cccgtcacca gtgtggggcc
     3361 cagcgcgatg tagggaacca gcacgccgaa ctgcaggtac gaccacttga cgacatcgaa
     3421 gtacaggtag tggaagaaca ggcggaagta atttcgccgc acgtcggcga acagcgtctt
     3481 cgccactggc ggcgccgcgc ggcccgcatc atcttctcca tagaccagtt ccttgcggta
     3541 ggcggcttcc acccgctggt tctggaattc caggcccggc agcttgatgc ccacgacggc
     3601 catcagcacc gtgcctgcaa gggcgaagcc gatcgcgacc cacaccagcg aatgcgacac
     3661 cggcccgagc cagggcagtt ccgtgacttt ggccgagagc gtccaaagga tgggaaggaa
     3721 ggcgaacagt gtcatcacac tgcgcatgaa atcgacgccc aggccctcca tgagcctggc
     3781 aaaccgcatc gtgtcttcct gcacccgctg tgccgcgccc tcgatgttgc gaagccgttc
     3841 cca
//
LOCUS       DASWIY010000035         4073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_24442, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000035 DASWIY010000000
VERSION     DASWIY010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24442"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1736)
                     /locus_tag="VGA59_01315"
     CDS             complement(201..1736)
                     /locus_tag="VGA59_01315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX9718343.1"
                     /translation="MKLTSFFRVAALLAQTAMLMYAPSSLAQAGGAPATHPSAQSRPI
                     PGRYIVVFKSGVDNPGAAAAGLMQGSGGQLHHTYTSAIKGFAASLPDAALQGIRNNPF
                     VDYIEQDQTVSLAQVSSPQNQAIWGLDRVDQADRPLDTQYNFSNTGAGVYAFILDTGL
                     RADHVEFAGRVRPGFNVIADGNGTNDCHGHGTHVAGTVGGSTWGVAKGVTVIPVRVLD
                     CTAYGLWSGVIAGIDWVANSSLRPAVANMSIGGTPSSSLDAALAGAVSKGVTMVVAAG
                     NSGADACNYSPSRAPSAITVGATTTTDARASYSNTGTCVDIFAPGTGVVSASSADTTS
                     SRIMSGTSMASPHVAGVAALLLQANPTASPAAITAAITSNATPNRLTTVGTGSPNLLV
                     YSAGSSSPVVQPTTQTVAFKSMVGNATRKGRNWRASAVVTVRDVNSSATVANATVNGG
                     FSPGGNASCVTGGSGSCTLTSGAIKLNAAQSTVLSGSGISGTLLNYDATQNAVSQIVI
                     SKP"
     gene            complement(2225..3316)
                     /gene="paaE"
                     /locus_tag="VGA59_01320"
     CDS             complement(2225..3316)
                     /gene="paaE"
                     /locus_tag="VGA59_01320"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEX9718344.1"
                     /translation="MAAHFHTLAVKRVTPDAAGSAAITLQVPAALREGFDFRPGQFVT
                     LRAAIAGQETRRSYSICSAHQRYVQSGEIDIGVKPVEEGVFSRWALEHLRAGETIEVM
                     APDGRFTPRVPGARHRVAFAAGSGITPVLSIMTSTLDSEPASRFTLVYGNQRVNTIMF
                     NEALQDLKDRYPARVSLIHLLSRQAQEVELLHGRIDDAKVAELLRTMLPAADIDEAFI
                     CGPEGMIDATQRALLAGGVPADRIHTERFFSTSQAPAKRAVPGDTPSAGVQTAAHRLD
                     VVLDGKTHQLAMGDDDRVLDVALEAGLDLPYSCKSGVCTTCRARLLEGEVEMEKNFTL
                     EQWEIDRGFVLTCQARPMTPRVVVSYDER"
     gene            complement(3327..3824)
                     /gene="paaD"
                     /locus_tag="VGA59_01325"
     CDS             complement(3327..3824)
                     /gene="paaD"
                     /locus_tag="VGA59_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX9718345.1"
                     /translation="MNAVQRAWQVLESVPDPEIPVVSIRELGILREVNERNGELEVVI
                     TPTYSGCPAMGQIEEDVRAALAANDLRGYVVTQLAPAWTTDWITDEARQKLRAFGISP
                     PHAAAQDDVVRFARRSAAVAQAVPCPHCGSANTTETSHFSSTACKALYKCLDCLEPFD
                     YFKPY"
     gene            complement(3821..>4073)
                     /locus_tag="VGA59_01330"
     CDS             complement(3821..>4073)
                     /locus_tag="VGA59_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX9718346.1"
                     /translation="TQEFWTVTSAEAEAVSSGTGADPCSLREAWDQEVDGALAEATLQ
                     RPRRQGHVPRGKEGLHSEHLGYLLAEMQGLARQHPGATW"
BASE COUNT          662 a         1275 c         1426 g          710 t
ORIGIN      
        1 ggtgctcgtg aggccgcggg tccaactgag atacgggttc gaggcaccaa gtacaaaccg
       61 acctctgtgc cggacgccgc agcccaatta cacatccgtt tcatgcgtcg cgggtcgcgc
      121 ggcgcgcggt ggagcaactg atatgcaacc tgctcaagcg cggggcgcag caagcaaggc
      181 gatgatcgct ctgcgcgatc tcagggcttg ctgatgacga tctggctgac ggcgttctgg
      241 gtcgcgtcgt aattcaggag cgtgcccgag atacccgagc cactcaaaac ggtgctttgg
      301 gcggcgttca gcttgatggc accgctggtc agcgtgcagc tgccactgcc gccggtcacg
      361 cagctggcat tgccaccggg cgagaagccg ccgttgacgg tggcgttcgc gaccgtggca
      421 ctactgttga cgtcacgcac cgtgaccacg gcggaagccc tccagttgcg gcctttcctg
      481 gtcgcattcc ccaccatgga cttgaaggca acagtctggg tcgtaggctg cacgacgggc
      541 gagctgctgc cagccgagta gaccagcagg ttcggcgaac cggtaccgac ggtcgtcagc
      601 cggttgggcg tggcattgga cgtgatggcg gccgtgatgg cggccggcga agccgttgga
      661 ttggcctgga gcagcagggc ggcgacgccg gcgacatgcg gcgatgccat ggacgtgccg
      721 ctcatgatcc tggaagacgt cgtgtcggcg ctcgaggccg acacgacgcc ggttccggga
      781 gcgaagatgt ccacacaggt gccggtattc gaataggacg cgcgcgcatc ggtggtggtg
      841 gttgcgccca ccgtgatggc gctgggcgcg cgggacgggg agtagttgca ggcgtcggcg
      901 ccgctgttgc ccgctgccac caccatggtc acgcccttgc tgacggcacc ggccagggcg
      961 gcgtccagcg aagaggacgg cgtgccgcca atcgacatgt tggccacggc ggggcgcaag
     1021 ctgctgttgg ccacccagtc gatgccggcg atcacgccgc tccagagtcc gtaagcggtg
     1081 cagtccagca cccgtaccgg aatcaccgta acgcccttgg ctacgcccca ggtggagccg
     1141 cccacggtcc ccgccacgtg ggtgccgtgg ccgtggcagt cgttggtgcc gttgccgtcg
     1201 gcaatcacgt tgaagccggg ccgcacacgg ccggcaaact ccacgtgatc ggcccgcagc
     1261 ccggtgtcca ggatgaacgc gtaaacgccg gcgccggtgt tgctgaagtt gtattgggtg
     1321 tccagcggac gatcggcctg gtcgacgcgg tccagccccc agatggcctg gttttgcggc
     1381 gatgagacct gggcgagtga cacggtctgg tcctgctcga tgtagtccac gaaggggttg
     1441 ttgcggatgc cctgcagcgc ggcgtccggc aagctggcgg caaagccctt gatcgcgctg
     1501 gtataggtgt ggtgcagctg cccgccactg ccttgcatca ggcccgcggc ggctgcgccg
     1561 gggttgtcca cgccgctctt gaacaccacg atgtagcgcc cgggtatcgg gcgcgactgg
     1621 gcggatggat gagttgccgg ggcgccgccg gcctgcgcca gcgagctggg cgcgtacatg
     1681 agcatcgccg tttgagcgag aagtgccgcg acgcggaaaa aagaagtcag tttcatgagg
     1741 atgggccgga agagccgctg ctgatgggcc ataggcccgg gggtgtaatt gatgagctgt
     1801 cagtggcacc agcccatccg tgcagccaat gctagtagga cagggctgac agcgacgtag
     1861 gaaaaaactg acaagacgta agcgaatgac ctcgttttta acaattgatg aggttgaaga
     1921 ttgggggctg cggttccatg ccgagctcgt ggttgggtgc attgctcagc agtaaaagcg
     1981 ggtcatctcc gcggctgagc ttgggcccga gcaaggactt ttgtcgcgtc gcgaacgcgc
     2041 cgcttcggcg cctgggagcg cgcaagaagt tacggcaggt tgcagctcga cagcgtcctg
     2101 gcgaaagctt tcgtttcgct caggacgggg gccgttgacg tgggtcaacg acagccgggc
     2161 gggacatatc gctggactgt gcggcgccgc agtccggtgt gcttcgttta gttagaagaa
     2221 gtgctcagcg ctcgtcgtag ctcaccacga cgcgcggtgt catcggccgg gcctggcagg
     2281 tcagcacgaa gccgcggtcg atttcccact gctcgagcgt gaagttcttt tccatctcca
     2341 cttcgccttc cagcagtcgc gcgcggcagg tggtacagac gccgctcttg catgagtagg
     2401 gcaggtccag cccggcttcg agggccacat cgagcacccg gtcgtcatcg cccatcgcga
     2461 gctggtgcgt cttgccgtcc agcaccacgt ccagccgatg ggcggcggtc tgtacgccgg
     2521 ctgacggcgt gtcgccgggc actgcccttt tcgcaggagc ttgcgaagtg gagaagaaac
     2581 gctctgtatg gatgcggtcg gccggcacgc caccggcgag cagggcgcgt tgcgtcgcgt
     2641 cgatcatgcc ctcggggccg cagatgaagg cctcgtcgat gtcggcagcc ggcagcatgg
     2701 tgcgcagcag ctcggcgacc ttggcgtcgt cgatgcggcc gtggagcagc tccacctcct
     2761 gcgcctggcg cgacaacagg tgaatcagcg agacccgcgc cgggtagcgg tccttcaggt
     2821 cctgcagggc ctcgttgaac atgatcgtgt tgacgcgctg gttgccgtag accagcgtga
     2881 agcgggatgc gggctcgctg tccagcgtgc tcgtcatgat cgaaagcacc ggcgtgattc
     2941 cggagccggc cgcgaaagcc acgcggtgcc gggcgccagg tacccgcggc gtgaagcggc
     3001 catccggtgc catcacctcg atcgtctcgc cggcgcgcag gtgctccagg gcccagcgcg
     3061 agaaaacccc ttcttccacc ggcttgacgc cgatatcgat ttcccccgat tgcacatagc
     3121 gctggtgcgc gctgcagatc gaatagctgc ggcgcgtctc ctggccggct atggcggcgc
     3181 gcagcgtcac gaactggccc ggccggaagt cgaagccttc gcgcagtgcc gccggcacct
     3241 gcagcgtgat cgcggccgaa ccggcggcgt cgggcgtaac gcgtttgacc gcgagggtat
     3301 ggaaatgcgc agccatcaga aagcgttcag tagggcttga agtagtcgaa gggctcgagg
     3361 caatccaggc acttgtagag ggccttgcac gctgtcgagc tgaagtggga agtttccgtc
     3421 gtattggccg agccgcaatg cgggcagggc accgcctggg cgacggcggc gctgcggcgc
     3481 gcgaaacgca ccacgtcgtc ctgggccgcg gcatgcggcg gcgagatgcc gaaggcccga
     3541 agtttctgtc ttgcctcgtc cgtgatccag tcggtggtcc aggcgggtgc cagctgcgtc
     3601 accacatacc cgcgcaggtc gttggccgcc agcgcggcgc gcacgtcttc ctcgatctgt
     3661 cccatggccg ggcagccgct gtaggtgggg gtgatcacca cttccagctc gccgttgcgt
     3721 tcgttcactt cgcgcaggat gccgagctcg cggatggaca cgaccggtat ctcggggtcg
     3781 ggcaccgact cgagcacctg ccaggcgcgc tgcaccgcat tcaccacgtc gccccgggat
     3841 gttggcgcgc caggccctgc atctcggcca gcaggtagcc caggtgctcg gaatgaagtc
     3901 cctccttgcc gcgcggaacg tggccttgcc gccggggccg ctgcaacgtg gcctcggcca
     3961 gtgctccatc gacttcctga tcccatgcct cgcgcaatga gcacgggtcc gcgccggtgc
     4021 cgctggaaac ggcctcggcc tcggccgacg tgacggtcca gaactcctgc gtg
//
LOCUS       DASWIY010000036         7291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1874911, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000036 DASWIY010000000
VERSION     DASWIY010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874911"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..98
                     /locus_tag="VGA59_01335"
     CDS             <1..98
                     /locus_tag="VGA59_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9718347.1"
                     /translation="AAVAEARGNVMQAAQALGISRATIYRKLGKK"
     gene            complement(281..1090)
                     /locus_tag="VGA59_01340"
     CDS             complement(281..1090)
                     /locus_tag="VGA59_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9718348.1"
                     /translation="MMSRAFRSGGLFVVGALLACAAWAQSITMASTTSTEQSGLFGHL
                     LPEFKKASGLDVKVVAVGTGQAIDMARRGDADVLFVHDRAAEEKFVAEGGAVQRFPVM
                     YNDFVLVGPKSDPAGTHGKDIVAALKKIAAAKAPFISRGDKSGTHAAELRYWNQAGLA
                     TAKGTGYKECGCGMGPALNIGSSANGYVLTDRGTWLSFKNRGDLLVLVEGDRRLFNQY
                     GVMVVNPAKYPHLKSADAQKFVDWITSPAGQSVIAGYKIGGEQLFFPNADR"
     gene            complement(1105..1806)
                     /locus_tag="VGA59_01345"
     CDS             complement(1105..1806)
                     /locus_tag="VGA59_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEX9718349.1"
                     /translation="MSGLIQLKAVDVRFGPVTALTDVDLGVGTGERLALIGANGSGKS
                     TLLRVLHGLVKPAAGSVLRDSATRQAMLFQRPYMLHTSVLHNVALPLWINGSRWKQAK
                     EQARLALGRVGLGEAALRNARALSGGQQQRLALARAWSLEPHVLFLDEPTSSLDPHGK
                     REVEALLAEFARGGMTLVFASHNLGQVKRLATRVVYLEQGRVLADLPVQEFFNGSRLV
                     ETSMEADLFVKGEMA"
     gene            complement(1803..2537)
                     /locus_tag="VGA59_01350"
     CDS             complement(1803..2537)
                     /locus_tag="VGA59_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9718350.1"
                     /translation="MNTFSDSAVTALALLAGFDPMLLSIVGRSLGVSGAACALSCALG
                     LPLGAWLGVARFRGRGALLTLLNTLLALPSVVVGLIVYLLLSRSGPLGFLGWLFSFKA
                     MVIAQTVLVLPVAAALTRQTVEDVDRAHGEQLQSMGAGPVLRSLLLALDERYALLTVV
                     IASFGRAIAEVGAVMIVGGNIDGFTRVMTTAIALETSKGDLPLALALGIVLLGVVLAL
                     NALIALVRRWRMVAIDQPLLLETAPS"
     gene            2670..3770
                     /locus_tag="VGA59_01355"
     CDS             2670..3770
                     /locus_tag="VGA59_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9718351.1"
                     /translation="MRKVQLSYALAPHGGSGTLSNALMDLLHAVREHGSISAAAKALG
                     LSYRHVWGELKRWEAELGHTLIVWDKGQPARLSEFGDKLLWAERQAQARLAPQIEALH
                     ADLERAFAVAFDDAAHVLTLFASHDDALAALREHCASNARLHLDIRFCGSVDAISALN
                     EGRCVMAGFHTPRLPGERTLAQRTYQPLLQPGRHKLIGFAQRTQGLIVATGNPLGLSG
                     LADVAGTGTRFINRALGTGTRVLLDELLARLKMSPAQLDGYDRTEPSHAAVAQAVASG
                     AADAGLGIEAAARSRGLGFVPLVQEDYYLVCLKAALEQDPVQALLQVLRSAAWQERVT
                     ALPGYQPERCGEVLSLRARLPWWDLPRKRIRK"
     gene            complement(3885..5138)
                     /locus_tag="VGA59_01360"
     CDS             complement(3885..5138)
                     /locus_tag="VGA59_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX9718352.1"
                     /translation="MPAVSPDTFPTSIDAPSMRHAGRELLSLALMDARNHSLHLLAHF
                     EKAALDVPLHLELELPAWLAGHIGWMAEYWIGRNPRRGLGPACPADTVRMASIEPMAD
                     RWFNPALVPHALRWQLELPQFEAIRAYLLETLESTLELLDKTPEDDESLFFYRAALFH
                     EDLRCEQLVTLAQTLELPLKLALPAGVQGRDAVLVPAGHWQLGSPAGGFVFDVEKWAH
                     DVDVPEFEIDAQPVSWGQYVEFVDDGGYDRPEVWLPQGWSWLQREAGQEGRRGPRYVE
                     QIGSASGAVMQTHFGKAARMAASQSVMHVSWWEADAWARWAGRRLPTEVEWEMAAHAA
                     ARRGFRWGDVREWTASTLRPWPGFSPDGWSLHTEFEAQPYFGRARVQRGASFAARSRM
                     KHPKFRGFALPERDDAFVGFRTCTL"
     gene            5329..5925
                     /locus_tag="VGA59_01365"
     CDS             5329..5925
                     /locus_tag="VGA59_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9718353.1"
                     /translation="MQALLKFSKAVDWLNAQVGKYVIWLILASTLISAVNAIVRKIFN
                     VSSNAYLEVQWYLFAAAFLLAAGYTLLHGEHVKIDVIYGKVSNRGQIWIDIFGFVAFL
                     TPMCFAILWYGIPFFLQGYRSGEVSSNAGGLIRWPVYLMIPLGFSLLLLQCWSELVKR
                     IAFLKGLIDDPRHKKTEKTAEEQLAEDIRALAEREGKV"
     gene            5952..>7291
                     /locus_tag="VGA59_01370"
     CDS             5952..>7291
                     /locus_tag="VGA59_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9718354.1"
                     /translation="MEFITGNLAPIMFLGLIVFLLIGFPVAFSLGACGLFFGFIGVEL
                     GVLPEALLQAMALRLFGIMQNDTLLAIPFFTLMGLILERSGMAEDLLDTIGQLFGPVR
                     GGLALAVILVGALLAATTGVVAASVISMGLISLPIMLRYGYDRRLASGAIAASGTLAQ
                     IIPPSLVLIILADQLGRSVGDMYKGAFIPGFILTGIYVSYVLMMALLKPNWAPALPPE
                     ARTFRQPDGSAGTPSLLAVTIISAAAAVTLAKNYAAVTSWVTGETVATVALDETIVVS
                     LCFGVLLAFVIAIINKLLHLGLLSRIAERVTFVLIPPLLLIFLVLGTIFLGIATPTEG
                     GAMGALGAFTMAIARRRLTFSLLRQALNATTKLACFVLFILIGATMFGLTFQGVDGPK
                     WVEHLLTGLPGGQLGFLIVVNLLIFVLAFFLDFFELSFIVVPLLAPVALTLGIDL"
BASE COUNT         1223 a         2483 c         2333 g         1252 t
ORIGIN      
        1 gcgccgccgt agcggaggcg cggggcaatg tgatgcaggc ggcgcaggcg cttggcatca
       61 gccgcgccac gatctaccgg aagctgggga agaaatagct ggcctcgcgg actccgcaac
      121 cagtaccggc gaacgcgaga aaggctgtca ttgcggactt gatccgcaat ccatcttctt
      181 ttgttcaacg cgcttgtcgg cgggcatgga tcccggatcg ggcctgcccc ggactcgatc
      241 cggggtccgg gatgacaaac aggaagacag acggcagtgg ctaccggtcg gcgttcggga
      301 agaacaactg ctcgccaccg atcttgtagc cggcgatgac cgactgcccc gccggcgaag
      361 tgatccagtc gacgaacttc tgcgcatcgg cggacttcag gtgcggatac ttcgccgggt
      421 tcaccaccat cacgccgtac tggttgaaca ggcgtctgtc gccttccacc aggaccaaga
      481 gatcgccccg gttcttgaag ctcagccagg tgccgcgatc ggtcagtacg tacccgttgg
      541 ccgaactgcc gatgttcagg gccgggccca tgccgcagcc gcattccttg tagcccgtgc
      601 ccttggccgt cgcgaggccc gcctggttcc agtagcgcag ctcggctgcg tgggtgccgc
      661 ttttgtcgcc gcgcgaaatg aacggcgcct tggcggcagc gatcttcttg agagccgcca
      721 cgatgtcctt gccgtgcgtg cccgccggat cgctcttggg gccgaccagc acgaagtcgt
      781 tgtacatcac gggaaagcgc tggacggccc caccttccgc aacaaacttt tcctcggccg
      841 cccggtcgtg cacgaacagc acgtcggcgt ctccgcggcg ggccatgtcg atcgcctggc
      901 cggtacccac ggcgacgacc ttgacatcga ggccgctggc tttcttgaac tccggcagca
      961 ggtggccgaa gaggccggac tgctcggtgg aagtagtgga agccatggtg atggactggg
     1021 cccaggccgc gcacgcgagc aaggcgccta caacgaacag gccgccggaa cgaaatgccc
     1081 gactcatcat cgatcgcgaa aaaatcatgc catttctccc ttgacgaaca gatcggcctc
     1141 catggaggtt tccaccagcc gcgacccgtt gaagaattcc tggacgggca agtcggccag
     1201 cacgcggccc tgttccaggt agaccacccg ggttgccagg cgcttgacct ggccgaggtt
     1261 gtggctggcg aacaccagcg tcatgccgcc ccgcgcgaat tcggccagca gcgcctcaac
     1321 ctcgcgcttg ccgtgcggat ccaggctcga agtgggttcg tccaggaaca gcacgtgcgg
     1381 ctcgaggctc caggcacggg ccagcgcgag ccgctgctgc tgcccgcccg aaagggcgcg
     1441 cgcgttgcgc agcgccgctt cgcccaggcc gacgcggccc aaggccagcc gggcctgctc
     1501 cttggcctgc ttccagcggc tgccgttgat ccacagcggc agtgccacgt tgtgcagcac
     1561 gctggtatgc aacatgtagg gccgctggaa cagcatcgcc tgtcgcgtgg cgctgtcgcg
     1621 cagtaccgag cccgccgccg gcttcacgag gccgtgcagc acccgcagca gcgtgctctt
     1681 gccactgccg ttagcgccga tgagcgccag ccgctcacct gtcccgacgc ccagatcgac
     1741 gtccgtcaac gcggtgaccg ggccgaaccg cacgtccacc gccttcagct gtatcaaccc
     1801 gctcatgagg gcgccgtctc cagcaagagg ggctggtcga tggcgaccat gcgccagcgc
     1861 cgcaccaggg cgatcagcgc attcagcgcg agcaccacgc ccagcaacac gatgcccagc
     1921 gccagggcca gtggcaggtc gcccttgctg gtctccagcg cgatggcggt ggtcatgacg
     1981 cgggtgaaac cgtcgatgtt gccgccgacg atcatcacgg cgccgacctc cgcgatggcg
     2041 cggccgaacg aagcgatcac taccgtcagc agcgcgtagc gctcatccaa ggccagcagc
     2101 aggctgcgca acaccgggcc ggcgcccatc gattgcagct gctcgccgtg cgcgcgatcg
     2161 acatcctcca ccgtctggcg cgtcagtgcc gcggccaccg gcagcaccag caccgtctgc
     2221 gcgatcacca tcgccttgaa cgaaaacagc cagcccaaaa atcccagcgg gccggagcgt
     2281 gacagcagca agtagacgat cagcccgacc acgaccgagg gcagcgccag caaagtgttg
     2341 agaagcgtca acagcgcgcc gcgcccgcgg aagcgggcca cgcccagcca ggcccccagc
     2401 ggcaggccaa gggcgcacga gagcgcgcaa gccgcgccgc tgacacccag cgagcgcccc
     2461 acgatcgaaa gcagcatggg atcgaaacct gcgagcagcg caagcgcggt gaccgcgctg
     2521 tcggaaaacg tgttcattcg gcattcatgc taagcggtcc ggagcggcgg cgcatgaggg
     2581 ctagcgagct atgaaccggc ttgcatagcc gcagtccggc gcgaaccacg cgggctgcgc
     2641 cttggcggac ggatgcatac tcagccaccg tgcgcaaggt ccaactctcc tacgccctgg
     2701 cgccccatgg cggctcggga accctcagca atgcgctgat ggacctgctg catgccgtgc
     2761 gcgagcacgg ttccatctcg gccgccgcca aggcgctggg cctgtcgtac cgccatgtct
     2821 ggggcgagct caagcgctgg gaggcggaac tcggccacac gctgatcgtc tgggacaagg
     2881 gccagccggc acgcctgtcg gagttcggcg acaagctgct gtgggccgag cgccaggcgc
     2941 aggcgcgcct ggcgccgcag atcgaggcgc tgcacgccga cctggaacgc gcgttcgccg
     3001 tcgctttcga cgatgccgcg catgtgctca cgctgttcgc cagccacgac gacgcgctgg
     3061 ccgccctgcg cgagcattgc gcatcgaatg cccgccttca cctggacatc cgtttttgcg
     3121 gcagtgtgga cgccatcagc gcgctgaacg agggtcgttg tgtgatggcg ggcttccaca
     3181 cgccgcggct gccgggcgaa cgcacgctcg cccaacgcac ctaccagccc ttgttgcagc
     3241 ctggccggca caagctcatc ggattcgcgc agcggaccca gggcttgatc gtcgcaacgg
     3301 gaaatccgct ggggctgtcg gggctcgctg acgtggcagg caccggaacc cgcttcatca
     3361 atcgagcgtt gggcaccggc acccgcgtcc tgctcgacga actgctggcc cgactaaaaa
     3421 tgagccccgc gcagctagac ggctatgaca ggaccgagcc gtcgcacgcg gcggtggcgc
     3481 aggccgtggc gtccggcgcg gccgacgctg gtctggggat cgaagcggcg gcgcgctcgc
     3541 gcggtcttgg tttcgtgccg ctcgtgcagg aggactacta cctcgtctgc ctcaaagccg
     3601 cgctggaaca ggatccggtg caggccttgc tgcaggtgct gcgcagcgcc gcctggcagg
     3661 aacgtgtgac ggcgctgccc ggctaccaac cggagcgctg cggcgaggtc ctgagcctgc
     3721 gcgcgcggct gccttggtgg gatttgccgc gcaagaggat caggaagtag cggcgctgcc
     3781 tgtcattact tcgcttgacc cgcaatccat gttggagcgc catttcattc gaagcatgga
     3841 tgccgggtca agcccggcat gacaagactc ttcttccagg ctcgctacaa cgtgcaagtg
     3901 cgaaagccca cgaaggcatc gtcgcgctcc ggcagcgcga agccacggaa tttcgggtgc
     3961 ttcatgcgcg agcgggcggc gaacgatgcg ccgcgctgga cccgtgcgcg gccgaagtac
     4021 ggttgcgcct cgaactccgt gtgcaggctc cagccgtcgg gcgagaaacc gggccagggc
     4081 cgcagcgtgc tggccgtcca ctcccgcacg tcgccccagc gaaagccgcg gcgggcggcc
     4141 gcgtgggccg ccatttccca ttccacttcc gtcggcaggc ggcggcccgc ccagcgggcc
     4201 cacgcgtccg cctcccacca gctgacgtgc atgacgctct ggctcgccgc catgcgcgcg
     4261 gccttgccga aatgcgtctg catcaccgcg ccgctggccg agccgatctg ctccacatag
     4321 cgcggcccgc gccgcccctc ctgaccggcc tcgcgttgca gccagctcca gccctgcggc
     4381 agccagacct cggggcggtc gtagccgccg tcgtcgacga actccacata ctggccccag
     4441 ctgaccggct gcgcatcgat ctcgaattcc ggcacgtcga cgtcatgcgc ccacttctcc
     4501 acgtcgaata cgaaaccgcc ggccggcgag cctagttgcc aatgacccgc gggcaccagc
     4561 acggcgtccc gtccctgcac ccccgcgggc aaagccagct tgagcggcag ctccagcgtt
     4621 tgcgccagcg tcaccagctg ctcgcaccgg aggtcttcat ggaacagcgc ggcccggtag
     4681 aagaacaggg attcgtcgtc ttcaggtgtc ttgtccagca gctccagcgt gctttccagc
     4741 gtttcgagca gataggcccg gatggcttcg aactgaggaa gctccagttg ccagcgcagg
     4801 gcatgcggca ccagcgcggg attgaaccag cggtcggcca tgggttcgat cgaagccatg
     4861 cgcaccgtgt cggcagggca ggccggtccc aggccgcgcc gcggattgcg gccgatccag
     4921 tattcggcca tccacccgat atggcccgcc agccaggccg gcagctccag ctcgagatgc
     4981 agcggcacgt ccagagccgc cttctcgaaa tgggcgagca ggtgcaagct gtggttgcgt
     5041 gcgtccatca gggccagcga caatagctcg cgtccggcgt gccgcatgga aggcgcgtcg
     5101 atcgaggtgg ggaacgtgtc gggcgagacg gcaggcatga gaacctaaag agcggacaag
     5161 ttcttcatta tcactttcgc gtacagtggc tggcgtcgtc aaaaagttgt cagcctgacc
     5221 tttatagtta accatagaat ttcttagtac aaaaccgggc gcgcaactga ccaacgcagt
     5281 gcgcgcccgg tgtcgcgtgg ccagtgaact tatttctccg gagacaacat gcaggcatta
     5341 ctcaagtttt ccaaggccgt cgactggctc aatgcgcagg tcggcaaata cgtgatctgg
     5401 ctgatcctgg cctcgactct catcagcgcg gtcaacgcca tcgtgcgcaa gattttcaac
     5461 gtcagctcga acgcgtacct ggaggtgcag tggtatctgt tcgcggcggc gttcctgctc
     5521 gcggcgggtt acaccctgct ccacggcgag catgtgaaga tcgacgtcat ctacggcaag
     5581 gtgtccaacc ggggccagat ctggatcgac attttcggtt tcgtcgcctt cctcacgccc
     5641 atgtgcttcg ccattctctg gtacggcatc cccttcttcc tgcagggcta ccggtcgggc
     5701 gaagtgtcgt ccaacgcggg gggactgatc cgctggcccg tctacctgat gatccccctg
     5761 ggcttttcgc tcctgctgtt gcaatgctgg tccgagctgg tcaagcgcat cgcattcctc
     5821 aagggcttga tcgacgatcc gaggcacaag aaaaccgaga agaccgccga ggaacaactg
     5881 gccgaagaca tccgggccct cgccgagcgc gaaggcaaag tctgaacaac aactagaacg
     5941 aggagcacaa gatggagttc atcaccggca acttggcccc gatcatgttc ttggggctga
     6001 tcgtcttcct gctgatcggg tttcctgtcg ccttcagcct gggcgcctgc ggcctgttct
     6061 tcggtttcat cggcgtggag ctcggcgtcc tgcccgaggc attgctgcag gccatggcac
     6121 tgcgcctgtt cggcatcatg cagaacgaca ccctgctggc aatcccgttc ttcacactga
     6181 tgggcctcat tctggagcga agcggcatgg ccgaggacct gctggacacc atcggccagc
     6241 tgttcggccc tgtgcgcggc gggctggcgc tggccgtgat cctggtcggt gcgctgctgg
     6301 cggcgaccac cggcgtggtg gccgcttccg tcatttccat gggcctgatc tcgctgccga
     6361 tcatgctgcg ctacggctac gaccggcgcc tggcctccgg cgccatcgcg gcctcgggca
     6421 cgctggccca gatcatcccg ccgtcgctgg tgctgatcat cctggccgac cagctgggcc
     6481 gcagcgtcgg cgatatgtac aagggggcgt tcatccccgg cttcatcctc accggcatct
     6541 atgtcagcta cgtgttgatg atggcgctgc tcaagccgaa ctgggccccc gcgctgccgc
     6601 cggaggcacg cacctttcgc cagcccgatg gttccgcggg gacgccatcg ctgctcgcgg
     6661 tgacgatcat ttccgctgcc gcggcggtga cgctggccaa gaactacgct gcggtgacga
     6721 gctgggtcac gggcgagacg gttgccaccg tcgccctgga cgagaccatc gtggtgtccc
     6781 tgtgcttcgg tgtgctcctc gcgttcgtga tcgcgatcat caacaagctt ctccacctcg
     6841 gcctcctgtc gcgcatcgcg gaacgggtga cctttgtcct gatcccgccg ttgctgctga
     6901 tcttcctggt gctgggcacg attttcctgg gcatcgccac gccgacggag ggcggtgcga
     6961 tgggtgcgct cggcgcattc accatggcga ttgcgcgcag gcgcctgacc ttctcgctgc
     7021 tgcggcaggc cctgaacgcc accaccaagc tggcctgctt cgtgctgttc atcctgatcg
     7081 gcgcgaccat gttcggcctt accttccagg gggtggacgg gccgaaatgg gtggaacacc
     7141 tgctcaccgg cttgccgggc ggccagctgg gcttcctgat cgtggtcaac ctgctgatct
     7201 tcgtgctggc cttcttcctg gacttcttcg agctctcctt catcgtggtg ccgctgctgg
     7261 cgcccgtggc cttgacgctc ggcatcgacc t
//
LOCUS       DASWIY010000037         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2607051, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000037 DASWIY010000000
VERSION     DASWIY010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2607051"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VGA59_01375"
     CDS             complement(<1..1034)
                     /locus_tag="VGA59_01375"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX9718355.1"
                     /translation="MRQGRLGIKAIATGTAAVLLTAAAALPALAQQKPPIMVPLLLCP
                     WGCGNTESSTILMNQMIRDGSPVTLLPQETPGYVYNIREMNQQKHWQKSAFGTEDVLI
                     QLAMKWGGTPELKEFLPVKVPVKFKLLYGEAWWGQGKFFVTYDPNIRSPADFKGKRIA
                     LGLRGQSDWGVFSRLFLEYGYGITPQNADIRHLTPAALTQQLIDGSADVAITAFGTEP
                     NLREWLIPAPLRQLEAAPKPLRYIGVSKEAIDKVNAKFGTTFLHITVPAGALPKLTQP
                     LSVAIVRGFMAVHPDFSEEAAYHIVTSVAKHAPKMRELHALWKIWSPELMVHGLSEEN
                     VHPGAKRAFV"
     gene            complement(1055..>1959)
                     /locus_tag="VGA59_01380"
     CDS             complement(1055..>1959)
                     /locus_tag="VGA59_01380"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9718356.1"
                     /translation="VDVQAILRTLPAFLVSFVVVCWLLIDYYSPSVAGFAGCIVALAL
                     CLFQGRYRPGARQLALALEGGLEMMAVLALLLIAIGPLGQAFLTTGLSGRLGSWLISV
                     LPESTLVLLIGAAVLSLILGMGLPTPVAYLVVALALVPFLQQLGLKPLLAHYFVFYFA
                     VYSALTPPVMVAVLAGAKLAGAGNWETAKESMKLAATTFIIPFAFVYRPQLLDFPNLG
                     WNVVPPLVEVLLIQWTSSIAFYGYFRRALTHVESAFFGAVTALGYWAMITEAPSSTVV
                     FLSATAAVMLAVAVRTPHPAFSRT"
BASE COUNT          359 a          634 c          648 g          318 t
ORIGIN      
        1 tcgacgaagg cccgcttcgc tcccgggtgg acattctctt ccgaaagccc gtgcaccatc
       61 agttccggcg accagatctt ccacaaggcg tgcagctccc tcatcttcgg cgcgtgcttg
      121 gccaccgagg tcacgatgtg ataggccgct tcctcggaaa agtcaggatg cacggccatg
      181 aagccgcgca cgatggcgac cgacagcggc tgggtgagct tcggcagcgc gccggccggg
      241 acggtgatgt ggaggaaagt ggtgccgaac ttggcgttga ccttgtcgat cgcctccttg
      301 gacacgccga tgtagcgcag cggcttcggc gcggcctcga gctggcgcag cggcgcgggg
      361 atcagccact cccgcaggtt gggctcggtg ccgaaagccg tgatcgcgac gtcggccgag
      421 ccgtcgatga gctgctgggt cagcgccgcc ggcgtgagat gccggatgtc ggcgttctgc
      481 ggcgtgatgc cgtagccgta ctcgaggaac agccgcgaaa acacgcccca gtcgctctgg
      541 ccgcgcagcc cgagggcgat gcgcttgccc ttgaagtcgg ccggcgaccg gatgttcggg
      601 tcgtaggtga cgaagaactt gccctggccc caccaggcct cgccgtagag cagcttgaac
      661 ttcacgggga cctttacggg caagaactcc ttcagctcgg gcgttccgcc ccatttcatc
      721 gcgagctgga tgagcacgtc ctcggtgccg aaggcgctct tttgccagtg cttctgctgg
      781 ttcatctcgc ggatgttgta cacgtagccg ggcgtttcct gcggcagcag ggtcacgggg
      841 ctgccgtcgc ggatcatctg gttcatcagg atggtgctgc tttcggtgtt cccgcagccc
      901 caggggcaca gcagcagcgg aaccatgatc ggcggcttct gctgtgcgag cgccggcagc
      961 gctgcggcgg cagtaagcag taccgccgcc gttcctgtcg cgattgcctt gattcccaag
     1021 cgtccttgtc tcatttgctc ctccttgcga gtggttacgt ccgtgaaaag gccggatgtg
     1081 gtgtgcggac cgcgaccgcc agcatgacgg cagcagtcgc gctcagaaac acgactgtcg
     1141 aggaaggcgc ctccgtgatc atcgcccagt agccgagggc cgtgactgcg ccaaaaaacg
     1201 cgctctccac gtgcgtaagg gctctccgga agtagccgta gaaggcaatc gagcttgtcc
     1261 attggatgag caggacttcg accagcggcg gcaccacgtt ccagcccagg ttcgggaagt
     1321 cgagcagctg cggccggtag acaaacgcga acgggatgat gaacgtcgtc gcggcgagct
     1381 tcatcgactc cttggccgtt tcccagttgc ccgcgcctgc cagcttggcg cccgccagca
     1441 ccgcgaccat gaccggcggc gtgagggcgg aatacacggc gaagtagaag acgaaatagt
     1501 gcgcgagcag cggcttcagt ccgagctgct gcagaaacgg caccagcgcg agtgccacca
     1561 ccaggtacgc caccggcgtg ggcagcccca tgcccaggat gagcgaaagc accgccgcgc
     1621 cgatcagcag taccagcgtg ctctcgggca gcacggagat gagccagctt ccaagtcttc
     1681 ccgaaaggcc ggtcgtcagg aacgcctggc cgagcgggcc gatcgcaatc agcagcagcg
     1741 caagcaccgc catcatctcc agccctccct cgagggccaa cgcgagctgc cgcgccccgg
     1801 gccggtacct gccctggaac aggcacagcg cgagggcgac gatgcagccg gcgaaccccg
     1861 ccaccgacgg cgagtagtag tcgatgagca gccagcagac gacgacgaag gacaccagga
     1921 aagcgggcag cgtgcgcaag atcgcctgga catcgacct
//
LOCUS       DASWIY010000038        18177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2607800, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000038 DASWIY010000000
VERSION     DASWIY010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 18177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18177
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2607800"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VGA59_01385"
     CDS             complement(<1..1091)
                     /locus_tag="VGA59_01385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9718357.1"
                     /translation="MKTIRLKAGKERSLLRRHPWIFDSAIARGGADTGETVRVESQDG
                     RFLAWAAYSPASKIRARVWTFEEGQRIDAAFLAAACQRSVQARSRFDIPSDGVRLVHG
                     ESDGLPGLIVDRYADTLVAQFLSAGAERWKPVLADALLRQTGLACLYERSDTGARSLE
                     GLAPAAGWLRGAPQGGPAPPTELTIREHDWRLTLDIAGGHKTGFYLDQRDSRGKFAAF
                     ARRLRFQRVLNCYCYTGGFTVAALAGMRAAGAAGAAGHVTSIDSSAPALERARSHVVL
                     NGFDPAQTDFIDADVNASLRRFIEEGRGFDAIVLDPPKLAPTAAHADRAARAYKDINR
                     LALKLLEPGGVLFTYSCSGAIGADLFHKIV"
     gene            1177..2892
                     /locus_tag="VGA59_01390"
     CDS             1177..2892
                     /locus_tag="VGA59_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX9718358.1"
                     /translation="MGFAECGRRFAAAALLLLAACMAMPALARTVLDLDTATQPVALL
                     DWGDAWTDPKGQAQAAQVAADDRIAWTPTRDGAIYPLTTGKALWFRFTVPPAPDAERW
                     YLEIPYPSVDGATLYTLDSAGQWLPQTAGDRLPVAQWPVPHRHPLLPVVVSAEEPRKY
                     LLRIENPHSFSAPLSFVSESYLSRHEQRTSLILGIYFGLVGLAVTLGVLSAISLRDEA
                     YAFYALSVGLMGLSQAAMTGIAGLHLWPQLPRWNDLSSMVLPVLAVGSMLWFFAAVVS
                     LPERSAILHRVATALALASLAVAAGIALVEPSHRFKLLVPYIVMASILGPAGIVWAAR
                     RGDRYALWLLVGTVPVIIGAAFPLARLSGLIPVSFWTMYGMQVGIAIELPVLLVILMV
                     RSQQRREHNRRIQGLDRIDPTTGLINGLVFEERLARMAARSRRLKYRSAVLLIDIVNI
                     EQIRRDFGARSAEELPLRVAGRLLAAAREIDGVARLSPLRFGMLVEGPLTAEEAASTG
                     PRLVARCLMPFQNKPLEWVAQVRVAQTLVPTERQEVQPVVERLGALLASVRPDNKRAV
                     FTLAG"
     gene            complement(2889..3812)
                     /locus_tag="VGA59_01395"
     CDS             complement(2889..3812)
                     /locus_tag="VGA59_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX9718359.1"
                     /translation="MDHLTERYLEHVRVEKRLAARTVELYALDLQKLSDHAAKAGVEL
                     THVQNGHVRRWVAQMHGAGRSGRGIALILSGWRGFYAWLGRQGLVASNPVQDVRAPKA
                     PKPLPKALSVDDSVQLATYLNTDGDPWLDARDAAMVELLYGCGLRVGELVGLDAAAST
                     TARGWIDLQACEAHVLGKGSKRRTVPVGRKAMEALIRWLAVRDKSGGEPQAALFVGRH
                     GTRLTAQAIWQRLKRRSLLAGLATPVHPHMLRHSFASHLLQSSGDLRAVQELLGHANI
                     TTTQVYTRLDFQHLAKAYDAAHPRAHRKPRS"
     gene            complement(3826..4518)
                     /locus_tag="VGA59_01400"
     CDS             complement(3826..4518)
                     /locus_tag="VGA59_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX9718360.1"
                     /translation="MKADGMNPITEDDIANYLANTPDFFARQAQLLASVQLTSPHGSR
                     AVSLQERQAEMLREKIKALEHRLMDMVRHGNENVIIADRLHHWACKLFLVQSGRALPA
                     TIAAELRHQFIVPQVAIKVWDVDSRYGAEPFAQGVSEDAKTFASSLTQPYCGVNSGFE
                     AVNWLDDTAMAMSLALIPLREGPVNSTGPAFGMLVLASPDAQRFQSGMGTEFLERIAE
                     LASAALSRLRAE"
     gene            complement(4515..5402)
                     /gene="dapF"
                     /locus_tag="VGA59_01405"
     CDS             complement(4515..5402)
                     /gene="dapF"
                     /locus_tag="VGA59_01405"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX9718361.1"
                     /translation="MRIRFTKMQGAGNDFIVLDETRGPLNLTPGQYRFLADRHFGVGA
                     DQVLSVRPSPTAGIDFEYVIHNADGGQVEQCGNGARCFMRFVRDKGHTSRDSVKVSTV
                     SGVIEPRLNPDGRVTVDMGPPVFDPVVIPFDDAGLSPQPDGQWEKWHLVLGTSADAAS
                     ISVAILSMGNPHAVLEVPDVDTAPVRTQGPLIENHPRFPNRVNVGFLQVVSRSRIRLR
                     VWERGAGETLACGTGACAAVVAGIRLGRLDGKVDVETHGGMLTVEWAGEGPQGAAPVL
                     MTGPAATVFEGEIEVPQSL"
     gene            5529..7163
                     /locus_tag="VGA59_01410"
     CDS             5529..7163
                     /locus_tag="VGA59_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9718362.1"
                     /translation="MVRPTQLLHAQRVPVAPRPAATVLLLRDTPQGLEVLMTRRSPSA
                     SFAPGAYVFPGGVIDAADGQAHDAAARRPTQDDLRLTQAVAAIRESFEELGILLACHA
                     DGRPASAQDIAAIDRHAPFVPQCRALGLTLAADQVYVLAHWITDLDLPRRFDVPFLVA
                     RMPAGQAPVADETEQFEPVWVQPADALARHAAGTFFMVFPTIRTLERLAKFDATGAVL
                     QACANEQPLWTSCPRAGLLKGAEARYMEHEMPYGELALVSPDGHIVHHLDWRTDESVP
                     LLRNVTRLTAPNPGFMTGPGTNSYLVGDPDTGYLAIDPGPNEPEHIQRLWRAAGGDIR
                     MIVCTHSHPDHSPGARPLQALCAGKPPVLGLPSAATARPHSEFRPDRALQDGETLVLE
                     GGGTRHSLKVIHTPGHAANHLCLVLLEDGLLFSGDHILNGSTTVVDPPDGDMTAYIDS
                     LDLLAAACRTHGIEFILPAHGHVLGFAAQAVAHLKAHRLKREAKIIAAMQARPDGALD
                     DWVELAYDDTPQRMWPVAKRSLLAHVQRIESLGLAP"
     gene            7177..7887
                     /locus_tag="VGA59_01415"
     CDS             7177..7887
                     /locus_tag="VGA59_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEX9718363.1"
                     /translation="MTEPVRLSKRVAALVPCSRREAEQYIEGGWVRVDGKVIEEPQFR
                     VADERIEIDPGARPAALEPVTLLLHKPAGIPYGQAQGLLVPAARSQDDRSEIRTVKRH
                     FAQLAPLVPLPPQASGLAVFSQDGRVVRKLTEDALAIEQELVAEVTGEAPPHGLGLLG
                     HGLAFEGRPLPPVKVSWQSETRLRFALKGIAPGLVPWMCEQVGLHVTALKRIRLGRLP
                     MAGLAPGQWRYLRPGERF"
     gene            7939..9201
                     /locus_tag="VGA59_01420"
     CDS             7939..9201
                     /locus_tag="VGA59_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HEX9718364.1"
                     /translation="MQNDHAAPKPAASARTIDTQGRDEGIQSFEIAFTGSGGEFFRVW
                     IVNLLLSIVTLGFYTPFARRRTAQYFYSHSLVADSPLEFTAQQRKMVFGFLLLVVLYL
                     AFRLAADTGQDTAVSLFLLAGAALAPYLWASAMRFRLSATRWRGVRLQFTAGWGEVYK
                     ASWPMFAIAGVWIVAMLAFTLFTGDVPVPIDDASSFEWPAVPPVLWALLVAALVVTLL
                     CIIRLEFNYKSLLVSKAYIGAQAGRWKPVYGDFVRIWLATLGVFIAGALLFALVVGLI
                     AGGSFLGAAGSKPAGPAATIVTMIAGVLAFFVGLLLASAPARAYRQARMFQLVWNNVG
                     VSRIARFKCKLRVSSFVLLRVKNMLLTLFTLGFYRPFAMVSEYRMKMESVTLHVKGGL
                     DQLVGQLVRQQGGLGDAVADAVGLDLVG"
     gene            9211..10314
                     /locus_tag="VGA59_01425"
     CDS             9211..10314
                     /locus_tag="VGA59_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX9718365.1"
                     /translation="MSEPIPARWFDGRSSRPQAVLVTLHSGPRGPSLHLHRLAQPAGE
                     PVRLEASQVGWPETWSAGRAPRSVVVDLRERGSLEIDDVRRWQAACASAGEQAPLARR
                     MQTRWTVFLVIALFAAAGLGAFYRWGTPWAATQITRQIPLAWETKFSARALRDLDEGW
                     LKPSRLPAERQRQLRERFDALARRIEPGMRRYGAYAPPLALSFRSGLGANAFALPGGT
                     IVITDALVEAAARQGLGDDALAGVLAHEIGHVMHRHTTRLVVEQGVLNVGLGLALGDV
                     SSLLSVGSSLLTGLAYRRGHETEADCFAAVLMQKARVPAAPMGDLLLGIEGAQAAHRK
                     VDTGAGGLASLLSSHPETALRAQKLKQGQVDGC"
     gene            complement(10395..10750)
                     /gene="rnpB"
                     /locus_tag="VGA59_01430"
     ncRNA           complement(10395..10750)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGA59_01430"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            10844..11761
                     /gene="cysK"
                     /locus_tag="VGA59_01435"
     CDS             10844..11761
                     /gene="cysK"
                     /locus_tag="VGA59_01435"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX9718366.1"
                     /translation="MKAANILETIGRTPHIRINRLFGAGHTVWVKSERSNPGGSIKDR
                     IALSMVEDAEQSGALKPGGTIIEPTSGNTGIGLAMVAAVKGYKLVLVMPDSMSIERRR
                     LMLAYGAAFELTPREKGMKGSIARAQELLAETPGGWIPQQFENPSNVEVHARTTAREI
                     LEDFPQGVDVLITGVGTGGHITGCARVLKSAWPKLKVFAVEPTASPVISGGQPSPHPI
                     QGIGAGFVPKNLDTSLLDGVIQVDAEPAREMARRAAREEGILVGISSGATLAAIAQKL
                     PDLPAGATVLGFNYDTGERYLSTEGFLPT"
     gene            11854..13533
                     /locus_tag="VGA59_01440"
     CDS             11854..13533
                     /locus_tag="VGA59_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9718367.1"
                     /translation="MIRLSEIKLTLAQAEQPQAALRAAAAHALRLHESDLGALQVFKR
                     SFDARKAELTAVYIVDVALADPAREAAVLAGFPGHPHIGPTPDMEWHPPVQVREALSE
                     RPVVVGFGPCGIFAALVLAQMGLRPIVLERGKKVRERTRDTWGLWRKRVLDPESNVQF
                     GEGGAGTFSDGKLYSQIKDPRHLGRKVMNEFVKAGAPEEILYVAHPHIGTFKLVKVVE
                     NMREQITALGGEIRFRHRLTDLLIEDGPDGKRLRGLTVLDQASGAVAEMRAEHVVLAL
                     GHSARDTFEMLHRRGVHMEAKPFSIGFRVEHPQSVIDRARWGRHAGHPLLGAADYKLV
                     HHANNGRSVYSFCMCPGGTVVAATTEAQRVVTNGMSQYSRNERNANAGMVVGIDPRDY
                     PGGPLAGVELQRQLESNAFVLGGGNYNAPGQLVGDFIAGRPSTQFGGVEPSYTPGVTL
                     GDLHGALPEYAIAALREALPAFGRKLRGFDMPDAVLTGVETRTSSPLKMTRGEDYQSV
                     NVRGLYPAGEGASYAGGILSAGVDGIKVGEALALDLVPSPANSSPLPLGEG"
     gene            complement(13597..14469)
                     /gene="htpX"
                     /locus_tag="VGA59_01445"
     CDS             complement(13597..14469)
                     /gene="htpX"
                     /locus_tag="VGA59_01445"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEX9718368.1"
                     /translation="MKRIVLFVLTNLAVVVVLGIVASLLGVNRYLTAQGLNLTSLLIF
                     SAIIGFGGAIISLLISKPVAKWSAGVQVIEQPRNQDEAWIVETVRRFANQAGIGMPEV
                     GIFQGEPNAFATGAFKNSALVAVSTGLLQGMTREEVEAVIGHEVAHVANGDMVTMTLI
                     QGVMNTFVVFLSRVISYAVDSFLSRGSSRSGPGIGYFVSTIVLDILLGFLAAIIVAWF
                     SRQREFRADAGSAQLLGRKQPMINALARLGGMVPGELPKSIQAFGITSGVGRLFSTHP
                     PIEERIAALQNAAV"
     gene            complement(14530..15339)
                     /locus_tag="VGA59_01450"
     CDS             complement(14530..15339)
                     /locus_tag="VGA59_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HEX9718369.1"
                     /translation="MSAAFLQQLAAVDVRHPWFAPYAGWLDAGRQAVQAGSSAAAALA
                     GLAPRESGVAVPEFIPAFNLTPGEAYEAFVARTGSVPTRDNLHDFFNGLAWLRFTRTK
                     RRLNELQAGEIARAGVGPRRGAVRDALTLFDENGALLAAPQPLWEALLARDWRRLFVQ
                     LRPLWSEAAVVVFGHALLEKLVSPRKDLTAHVWCANTAPVAGSPDRWLAEQLTAGRLS
                     EKPFTPLPLLGIPGWWAGNQDFSFYDDALVFRPAGRASQRAPARRSTASRT"
     gene            complement(15336..16100)
                     /locus_tag="VGA59_01455"
     CDS             complement(15336..16100)
                     /locus_tag="VGA59_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9718370.1"
                     /translation="MNESQRVALLIDADNVSADVIEQAIQRTLAEHGAIHVRRAYCNA
                     ETAVKQQALFKRLLVRPLVNLSAGKNSTDIALAVDAMDLVIAERPHVVVLVSSDSDFA
                     PLVIRLREKGCRVCGIGQRGKTGDETTAVYDAFTDIQHHGPRTSAPARARAPAPAPEP
                     APQPAGRAPRRARSRAEPTTPSVPEEVLRILDVVPELRGGARVELNQAAQLLRAGKLL
                     GKSASSLKLFRKHPELFVLSPDKRPSKVQFTPSQPA"
     gene            complement(16097..17128)
                     /gene="pyrC"
                     /locus_tag="VGA59_01460"
     CDS             complement(16097..17128)
                     /gene="pyrC"
                     /locus_tag="VGA59_01460"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX9718371.1"
                     /translation="MTQEITITRPDDWHVHVRDGEALKTVVPHTAAQFGRAIIMPNLR
                     PPVATAAQAMAYRERIREAVPPGMSFQPLMTLYLTDRLPPDEIVRAKEIGVVAVKLYP
                     AGATTNSDAGVTDLRNAYRTLEAMQREGLPLLVHGEVTDPAVDVFDREAVFLDTRLIP
                     LRRDFPELKVVIEHITTREAAQYVRDSDRFVAATITAHHLLYNRNAIFTGGIRPHYYC
                     LPVLKRETHRQALVEAATGGNPKYFLGTDSAPHPAHLKEHATGCAGCYTAHAAMELYA
                     EAFDDANALDRLEGFASLHGADFYGLPRNREKITLRRETWKPPESFAFGEATLKPLRG
                     GEPLSWRLA"
     gene            complement(17261..17998)
                     /locus_tag="VGA59_01465"
     CDS             complement(17261..17998)
                     /locus_tag="VGA59_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX9718372.1"
                     /translation="MIEIKNISKWYGPVQVLTDCSVNINKGDVVVVCGPSGSGKSTLI
                     KTVNALEPFQKGEIFVEGIPLHDPKTDLPKLRSRVGMVFQSFELFPHLSVTENLTIAQ
                     IKVLNRSPDEALARGLKMLDRVGLMAHKDKFPGQLSGGQQQRVAIARALSMDPIVMLF
                     DEPTSALDPEMVGEVLDVMVALAKEGMTMMVVTHEMGFARKVASRVIFIDVGGKILED
                     CTTDEFFGHPENRQPRTKDFLNKILAH"
BASE COUNT         2762 a         6250 c         6197 g         2968 t
ORIGIN      
        1 acgatcttgt ggaacaggtc ggcgccgatc gcgccggagc aggagtaggt gaacagcacg
       61 ccgcccggct ccagcagctt gagcgccagc cggttgatgt ctttgtacgc ccgggccgcg
      121 cgatcggcgt gggccgccgt gggcgccagc ttgggcgggt ccagcacgat ggcgtcgaag
      181 ccgcggcctt cctcgatgaa ccggcgcagc gacgcattga catcggcgtc gatgaagtcg
      241 gtttgcgcgg ggtcgaaccc gttcagcacg acatgggagc gggcccgctc cagcgccggc
      301 gcggacgaat cgatggaagt gacgtggccg gcggcgcccg ccgcacccgc agcgcgcatg
      361 ccggcgaggg ccgccacggt gaagccgccc gtgtagcaat agcagttaag cacgcgctgg
      421 aaccgaagcc gccgcgcgaa ggcggcaaat ttcccgcggc tgtcgcgctg gtccaggtag
      481 aaaccggtct tgtggccgcc ggcgatgtcc agcgtgagcc gccagtcgtg ttctcgaatg
      541 gtgagctcgg tcggcggcgc ggggccgccc tgcggcgcgc cgcgcagcca gcccgccgcg
      601 ggcgccaggc cttccagcga acgggcgccg gtgtcggagc gctcgtacag gcaggccagt
      661 cccgtttgcc tcaacagcgc atcggccagc accggcttcc agcgctccgc cccagcggag
      721 aggaattgcg ccaccagcgt gtcggcgtag cggtccacga tcagcccggg caggccgtcc
      781 gactccccgt gcaccagccg cacgccatcg cttggaatgt cgaagcgcga gcgcgcctgc
      841 accgaacgct ggcaggccgc ggcgaggaac gcggcgtcta tgcgctggcc ctcctcgaag
      901 gtccagaccc gcgcccggat cttcgacgcc ggactgtacg ccgcccacgc caggaaccgg
      961 ccgtcctgcg actccacccg cacggtttcg ccggtatcgg cgccgcccct ggcaatggcg
     1021 gaatcgaaaa tccacggatg ccggcgcagc agcgagcgct ccttgcctgc cttgagtcgg
     1081 atcgttttca tggcttgcat tgtccggcct ggcgccccgg ccttcgggcc aggcagccgc
     1141 cggggcacaa tcaggcgagt gaggaatatc aggaaaatgg gattcgcgga atgcggccgc
     1201 aggtttgcgg cagccgcgct gctgcttctg gccgcatgca tggcgatgcc cgcgctcgcc
     1261 cgcacggtgc tcgacctgga caccgccacg cagccggttg cgctgctcga ctggggcgac
     1321 gcctggacgg accccaaagg acaggcgcag gccgcccagg tggcggccga cgaccgcatc
     1381 gcgtggaccc cgacccggga tggcgccatc tatccgctga ccaccggcaa ggcgctgtgg
     1441 tttcgcttca cggttccgcc cgctcccgat gccgagcgct ggtacctgga gattccctat
     1501 ccgtcggtcg atggcgccac gctgtacacg ctcgacagcg ccggccagtg gctgcctcag
     1561 acggcgggcg accggttgcc ggtggcccag tggccggtgc cgcaccggca tccgctgctg
     1621 ccggtggtcg tgtccgcgga ggagccccgc aagtacctgc tgcgcatcga aaatccgcac
     1681 agcttcagcg cgccgctgag cttcgtcagc gaaagctacc tgagccgcca cgagcagcgc
     1741 acttccctga tcctgggcat ctacttcggc ctggtcgggc tggccgtgac gctcggtgtc
     1801 ctgagcgcga tttccctgcg cgacgaggcg tacgcctttt acgcgctgag cgtcggcctg
     1861 atgggcctga gccaggccgc gatgacgggc atcgccggcc tgcacctgtg gccgcagctg
     1921 ccccggtgga acgatttgtc ctcgatggtg ctgccggtgc tggccgtcgg ctcgatgctg
     1981 tggttctttg ccgcggtggt gtcgctgccc gagcggtcgg ccatcctgca ccgcgtggcc
     2041 acggcgctgg cgctggcgtc gctggcggtc gccgcgggca tcgccctcgt ggagccctcg
     2101 caccggttca agctgctggt gccctacatc gtcatggcgt ccatcctcgg cccggccggg
     2161 attgtctggg ccgcgcggcg cggcgaccgg tacgcgctgt ggctgctcgt gggcaccgtg
     2221 ccggtcatca tcggcgcggc ttttccgctg gcccgcctgt ccgggctgat cccggtgagc
     2281 ttctggacca tgtacggcat gcaggtcggc atcgccatcg agctgccggt cctgctggtg
     2341 atcctgatgg tgcgcagcca gcagcggcgc gagcacaacc ggcgcatcca gggcctggac
     2401 cgcatcgatc cgaccaccgg cctcatcaac ggcctggtat tcgaggaacg gctggcccga
     2461 atggccgcga gatcgcggcg gctgaagtac cgcagcgccg tgctcctgat cgatatcgtc
     2521 aacatcgagc agatccggcg cgacttcggc gctcgatcgg ccgaggagct gccgctgcgg
     2581 gtggcggggc gcctgctggc cgccgcccgc gagatcgacg gcgtggcgcg gctctcgccc
     2641 ctgcgattcg ggatgctggt ggaagggccg ctcacggcgg aagaagcggc ctcgaccggg
     2701 ccgcgcctgg tcgcccgttg cctgatgcct ttccagaaca agccgctcga gtgggtggcg
     2761 caggtgcgcg tggcccagac gctggtgcca acggaacgac aggaggtcca gccggtggtc
     2821 gagcggctgg gggccctgct ggcttcggtc cggccggaca acaagcgagc tgtgttcacc
     2881 ctggccggtt aggaccgggg cttgcggtgc gcgcgcgggt gcgccgcgtc gtaggctttg
     2941 gccagatgct ggaagtccag gcgggtgtaa acctgggtcg tcgtgatgtt cgcatgcccc
     3001 agcagctcct gcaccgcgcg caggtcgccg ctggattgga gcaggtggct ggcaaacgaa
     3061 tggcgcagca tgtgtggatg gaccggcgtc gccaggccgg ccagcaggct gcggcgcttc
     3121 aggcgctgcc agatggcctg ggccgtcagg cgggtgccgt ggcggccgac gaaaagggcc
     3181 gcttgcggct cgccgccgct cttgtcacgc actgccagcc aacgaatgag cgcttccatc
     3241 gccttgcgcc ccacgggcac cgtccgccgc ttgctgcctt tgcccagcac atgggcctcg
     3301 cacgcctgca agtcgatcca gccccgcgcc gtggtgctcg ccgccgcgtc cagtccgacc
     3361 agctcgccga cccgcaggcc gcagccgtac agcagttcga ccatcgcggc gtcgcgcgca
     3421 tccagccagg gatcgccatc ggtgttcagg taagtcgcca gctggaccga atcatccacg
     3481 ctcagagctt tgggcagcgg cttgggcgcc ttcggtgcgc gcacgtcctg gaccggattg
     3541 ctggccacca ggccctggcg gccgagccag gcgtagaagc cgcgccagcc ggaaaggatc
     3601 agcgcgatgc cgcggccgct gcggccggcc ccgtgcattt gcgccaccca gcggcgcaca
     3661 tggccgtttt gcacgtgcgt gagttccacc cctgccttgg ccgcatggtc cgaaagcttt
     3721 tgcaggtcca gcgcgtagag ttcgacggtg cgcgccgcga gccgcttctc gacccgaaca
     3781 tgctccaggt aacgctcggt caggtggtcc atgaccgtcc cggccctact ccgcgcgaag
     3841 cctcgacagc gcggcgctcg ccagctcggc gatgcgctcc aggaattcgg tgcccatccc
     3901 gctctggaag cgctgggcgt cgggcgaggc cagcaccagc atgccgaagg cgggcccggt
     3961 gctgttgacg gggccttcgc gcagcgggat caacgccagc gacatcgcca tcgccgtatc
     4021 gtccagccag ttcacggcct cgaagcccga gttcacgccg cagtacggct gggtcagcga
     4081 cgaagcgaag gtcttcgcgt cctcgctcac gccctgcgcg aagggctccg cgccataacg
     4141 gctgtccacg tcccagacct tgatcgcgac ctggggcacg atgaactggt ggcgcagctc
     4201 cgcggcgatc gtggcgggaa gagcgcgccc cgattgcacc aggaacagct tgcaagccca
     4261 gtggtgcagc cggtcggcga tgatcacgtt ctcgttgccg tggcgcacca tgtccatcag
     4321 gcggtgttcc agggccttga tcttttcgcg cagcatctcc gcctggcgct cctgcaggct
     4381 cacagcccgg ctgccgtgcg ggcttgtcag ctgcacggac gccagcagtt gcgcctggcg
     4441 ggcgaagaag tcgggcgtgt tggccaggta gttggcgatg tcgtcttcgg tgatcgggtt
     4501 catgccatcg gctttcatag tgattggggt acctcaatct cgccttcgaa caccgtcgcg
     4561 gccggtccgg tcatcaacac cggcgccgcc ccctgcgggc cttcgccggc ccattccacg
     4621 gtcaacattc cgccatgggt ctcgacgtcc accttgccgt ccagccgccc caggcggata
     4681 cccgccacca ccgcggcgca agccccggtg ccgcaggcca gcgtttcgcc cgcgccccgc
     4741 tcccacaccc gcagccggat gcgcgatcgg ctgaccacct gcaggaagcc cacgttcacg
     4801 cgattgggaa agcgcgggtg gttctcgatc agcggtccct gggtgcgcac cggcgcggtg
     4861 tccacgtccg gcacctccag caccgcatgc ggattgccca tcgacaggat cgccacggaa
     4921 atgctggcgg catccgcgct ggtgccaagc accaggtgcc acttttccca ttgcccgtcg
     4981 ggttgcggcg acaaaccggc gtcatcgaac gggatcacca ccgggtcgaa cacgggcggc
     5041 cccatgtcca ccgtgacgcg gccgtccggg ttcagccgcg gctcgatcac gccggaaacg
     5101 gtgctcactt tcacgctgtc gcgggaagtg tgtcccttgt cgcgcacgaa ccgcatgaag
     5161 cagcgcgcgc cgttgccgca ctgctcgacc tggccgccgt cggcgttgtg gatcacgtat
     5221 tcgaaatcga ttcccgccgt cggcgacggc cgcaccgaca ggacctggtc ggccccgaca
     5281 ccgaagtgcc ggtcggccag gaagcggtac tggcccggcg tgaggttcag cgggccgcgc
     5341 gtttcatcga gcacgatgaa atcgttgccg gccccctgca tcttggtgaa gcggatacgc
     5401 atgggcgcga ttatccgcgc agaggtccgc ctttcactcg gccttgacct tgttttcctt
     5461 caccccctgc gcccaggtgt cgatctgccg ggcagtagcc gattgcggcc cctgccgata
     5521 atggcgaaat ggtccgacct acccagcttc tccatgcgca gcgcgtaccc gtcgcgccgc
     5581 gccctgccgc caccgtcctg ctgctgcgcg atacgcccca aggactcgaa gtgctgatga
     5641 cgcggcgctc gccgtcggcc agcttcgcgc cgggcgccta tgtgtttccc gggggcgtca
     5701 tcgatgcggc ggatgggcaa gcccatgatg cggccgcgcg ccgaccgacg caggacgatc
     5761 tgcggctgac gcaggccgtc gcggccatcc gcgagagttt cgaggaactg ggtatcctgc
     5821 tggcgtgcca tgccgacggg cggcccgcca gcgcgcagga catcgcggcc atcgaccggc
     5881 acgctccgtt cgtcccgcaa tgccgcgcgc tcggcctgac gctggccgcc gaccaggtct
     5941 atgttctcgc gcactggatc acggacctcg acctgccgcg ccgcttcgac gtcccgttcc
     6001 tggtcgcgcg catgcccgcc ggccaggcgc ccgttgccga cgagaccgag cagttcgagc
     6061 cggtgtgggt gcagccggcc gacgcgctgg cccgtcacgc ggccggcacc ttcttcatgg
     6121 tcttcccgac catccgcacg ctggagcggt tggcgaaatt cgacgcgacc ggcgcggtgc
     6181 tgcaagcctg cgccaacgag cagccgctgt ggacgagttg tccgcgcgcc gggctgctca
     6241 agggggcgga agcgcgctac atggaacacg aaatgccgta cggcgagctc gcgctggtca
     6301 gccccgacgg ccacatcgtg caccacctgg actggcgcac cgacgagagc gtgcccctgc
     6361 tcaggaacgt gacgcggttg acggcgccca atcccggctt catgacggga cccggcacca
     6421 acagctacct ggtgggcgat ccggacacgg gttacctcgc gatcgatccc gggcccaacg
     6481 agccggaaca tatccagcgc ctctggcgcg cggcgggggg cgacatccgg atgatcgtct
     6541 gcacgcattc ccaccccgac cattcgcccg gcgcccggcc gctgcaggcg ctgtgcgcgg
     6601 gcaagccgcc ggtgctggga cttccttcgg ccgccacggc ccggccccac agcgaattca
     6661 ggcccgatcg ggcgctgcag gacggcgaaa ccctcgtgct ggagggcggc ggcacgcgcc
     6721 acagcctgaa ggtgatccat acgccgggcc acgcggccaa ccacctgtgc ctggtgttgc
     6781 tggaggacgg cctgctgttc tcgggcgacc atatcctcaa cggcagcacc accgtggtcg
     6841 atccgcccga cggcgacatg acggcctaca tcgattcgct ggacctgttg gccgccgcgt
     6901 gccgcaccca tggcatcgaa ttcatcctgc cggcgcacgg ccacgtgctg ggttttgccg
     6961 cgcaggccgt tgcgcacctg aaggcgcatc gcctgaagcg cgaggccaag atcattgcgg
     7021 cgatgcaggc ccgccccgat ggggcgctgg atgactgggt cgagctcgcc tacgacgaca
     7081 cgccgcagcg gatgtggccg gtggccaagc gctccctgct ggcccatgtg caacgcatcg
     7141 agtcgctcgg cctggcgcca tgagcgcgcc cagtccatga ctgaaccggt ccggctgtcc
     7201 aagcgcgtcg cggccctggt gccctgttcg cgccgggagg ccgagcaata catcgaaggc
     7261 ggctgggtgc gggtcgatgg aaaggtcatc gaggagcccc agttccgggt cgccgacgag
     7321 cggatcgaaa tcgatcccgg cgcgaggccg gccgcgctgg aaccggtcac gctgctgctg
     7381 cacaagcccg ccggcattcc gtacgggcag gcgcagggcc tgctcgttcc cgccgcgcgg
     7441 tcgcaggacg accgctcgga gatccgcacg gtgaagcggc atttcgcgca gctcgcgccg
     7501 ctggtgccgt tgccgccgca agcgagcggg ctggccgtgt tcagccagga cggccgggtg
     7561 gtgcgcaagc tcaccgagga cgccctggcg atcgagcagg agttggtcgc cgaggtcacc
     7621 ggagaagcgc cgccgcatgg cctgggcctg ctgggccacg gactggcgtt cgaaggccgg
     7681 ccgctgccgc cggtcaaggt gagctggcag agcgagaccc gcctgcgctt cgcgctcaag
     7741 ggcatcgctc ccggcctggt cccgtggatg tgcgaacagg tcggcctgca cgtcacggcg
     7801 ctgaaacgca tccgcctcgg ccggctgccg atggcgggcc tggcgccggg ccagtggcgt
     7861 tacctgaggc ccggcgagcg cttttagatt gcgaagaggc actgctaaac tgccaattcc
     7921 tggaggggga ggcaagaaat gcagaacgac catgccgcgc caaagccggc cgcgagcgcc
     7981 cgcaccatcg acacgcaggg gcgcgatgag ggtattcaat ccttcgagat cgcgttcacg
     8041 ggctccggcg gcgagttctt ccgggtctgg atcgtcaacc tcctgctgag catcgtcaca
     8101 ctgggctttt acacgccttt cgcgcggcgc cgcaccgcgc agtatttcta cagccactcg
     8161 ctggtggccg acagcccgct ggagttcacg gcgcagcagc gcaagatggt gttcggtttt
     8221 ctgctgctgg tggtgctcta cctggcattc aggctcgcgg ccgacaccgg gcaggacact
     8281 gccgtatcgc tgttcctgct ggccggcgcc gcgctcgcgc cctacctgtg ggccagcgcg
     8341 atgcgcttcc ggctgagtgc cacgcggtgg cgcggcgtgc ggctgcagtt caccgcgggc
     8401 tggggcgagg tctacaaggc gagctggccg atgttcgcca tcgccggagt ctggatcgtg
     8461 gcaatgctgg cgtttaccct cttcacgggc gacgtgccgg tgcccatcga cgatgcgtct
     8521 tccttcgagt ggccggccgt cccgcccgtg ctctgggcgc tgctggtcgc agccctggtc
     8581 gtgacactgc tgtgcatcat ccggctcgaa ttcaactaca agagcctgct cgtgtcgaag
     8641 gcctatatcg gcgcgcaggc agggcgctgg aagccggtgt acggcgattt cgtgcgcatc
     8701 tggctcgcga cgctgggggt atttatcgcg ggggcgttgc tgttcgcgct ggtggtcggc
     8761 ctgatcgcgg gcgggtcctt cctgggggcg gccggcagca agccggcagg cccggccgcg
     8821 accatcgtga cgatgatcgc cggcgtgctg gcctttttcg tcggattgtt gctggcctcg
     8881 gcgccggcgc gggcctatcg ccaggcgcgc atgttccagc tggtgtggaa caacgtgggg
     8941 gtcagccgca tcgcgcgctt caagtgcaag ctgcgcgtta gctcctttgt gctgctgcgc
     9001 gtgaagaaca tgttgctgac gctgttcaca ctcggcttct accggccctt tgccatggtg
     9061 agcgagtacc gcatgaaaat ggaatcggtg accttgcacg tcaagggcgg cctggaccag
     9121 ctggtcggcc agctggtgcg ccagcagggt ggcctgggcg atgcggtggc cgacgcggtc
     9181 ggcctggacc tggtcggcta gccacgcgcc atgagcgagc cgatcccggc gcgctggttc
     9241 gacgggcgca gcagccggcc gcaggccgtg ctggtgaccc tgcattcggg gccgcgcggg
     9301 ccttcactgc atttgcaccg gctggcacag ccggcaggcg agcccgtgcg actggaagcc
     9361 tcgcaggtcg gctggccgga aacgtggagc gccgggcgcg cgccgcgcag cgtggtggtg
     9421 gacttgcgcg aacgcggcag cctggaaatt gatgatgtgc ggcggtggca ggccgcttgc
     9481 gcgtccgccg gcgagcaagc gccgctggcg cggcgcatgc agacccgctg gaccgtgttt
     9541 ctcgtcatcg cgctgttcgc ggcggcggga ctgggggcct tctaccgctg gggcacgccc
     9601 tgggcggcga cgcaaatcac gcgccaaatc cccctggctt gggaaacgaa gttttcggca
     9661 cgggcactgc gggacctgga cgagggctgg ctcaagccga gcagattgcc ggccgagcgc
     9721 cagcgccagt tgcgcgagcg cttcgatgcg ctggcccggc ggatcgagcc cggcatgcgc
     9781 cgctatggcg cctacgcgcc gcctctggcc ttgtcgtttc gctccggcct gggcgccaac
     9841 gccttcgccc tgcccggcgg cacgatcgtg atcaccgatg ccctggtgga ggccgcggcc
     9901 aggcagggcc tgggcgacga cgcgctggcg ggcgtgctgg ctcacgagat cggccacgtg
     9961 atgcaccgtc acaccacgcg cctggtcgtg gagcaagggg tactcaacgt gggcctgggc
    10021 ctggcgctgg gcgatgtgtc cagcctgctt tcggtgggaa gctcccttct cacgggcctg
    10081 gcttaccgcc gcggccatga gacggaagcc gactgtttcg ccgccgtcct gatgcaaaag
    10141 gcccgtgtgc ccgccgcacc gatgggcgat ttgctgcttg gaatcgaagg cgcgcaagct
    10201 gcgcacagaa aggtcgacac cggcgccggc ggcttggcga gccttctcag cagccatccg
    10261 gaaacggccc tgcgggcgca aaagctcaag cagggccaag tcgacggctg ctagcggctg
    10321 ctcctgccgc gcacgctgcg cacgctgcgc acgctgcgca cgctgcgcgc gcatcggaca
    10381 gccgcgggaa ttaagtgtga agtccgccgt aagccggatt ctgtggcgtc cggttgcccg
    10441 gacgtgaccg ccattcctct gggctgcctg tcgccaggca gctcggtgct acctacccgt
    10501 gaacttccgg gggccccgga tggaaggacg cggcgcgagc cggaggtcac ttccgcgttc
    10561 acctacttgg tattgctgcg cgtagagatt gcccgtttca cccggcgtgc acgcacgccg
    10621 actcgtctct gttgctctga tcctcacctc gcggtggaca ggtgttacct gctacgctgc
    10681 cctgtgcagt ccggacgttc ctccagtgcc tggtttccca gattgcacca gcggcggtct
    10741 ggcgggcttc acggctgcat tatctccctg cggcatattc ttggcttgga actcgcataa
    10801 agcctgggtc agaatgcgtc tttgccaaac ccaggagacc gcaatgaaag cagcgaacat
    10861 tctcgagacc atcggccgca cgccccacat ccgcatcaac cgcctgttcg gggccggcca
    10921 cacggtgtgg gtcaaatccg agcgcagcaa ccccggcggt tcgatcaagg accgcatcgc
    10981 gctatccatg gtggaagacg ccgagcagtc gggcgccctc aagccgggcg gcacgatcat
    11041 cgagcccacc tcgggcaaca ccggcatcgg cctggccatg gtcgcggccg tcaagggcta
    11101 caagctcgtg ctcgtgatgc ccgacagcat gtcgatcgag cgccgccgcc tgatgctggc
    11161 ctatggcgct gccttcgaac tcaccccgcg cgagaagggc atgaagggtt cgatcgcgcg
    11221 ggcccaggag ctgctggccg aaacgccggg cggctggatt ccccagcagt tcgagaaccc
    11281 ctcgaacgtg gaagtgcatg cgcgcaccac ggcgcgcgag atcctggagg atttcccgca
    11341 gggcgtggat gtgctgatca ccggcgtcgg caccggcggg cacatcaccg gctgcgcccg
    11401 cgtgctcaag tccgcctggc ccaagctgaa ggtgttcgcc gtggaaccga cggcctctcc
    11461 cgtgatttcg ggtggacagc cttcgccgca ccccatccag ggcatcggcg cggggttcgt
    11521 gcccaagaac ctcgacacat cgctgctcga cggcgtgatc caggtcgatg ccgagcccgc
    11581 ccgcgagatg gcccggcgcg ccgcgcgcga agaaggcatc ctggtgggca tttcttccgg
    11641 tgcgacgctg gccgcgatcg cgcagaaact gccggacctg ccggccggcg ccacggtgct
    11701 gggcttcaac tacgacaccg gcgagcgcta cctctccacc gaaggtttcc tgcccacctg
    11761 agcggcccgg tcccgccgat gcgccgggcg cgccggcgcc gcccccgtat catgggcggc
    11821 ctgtctgctt gatcgacaca agccacggga accatgatcc gactctccga aatcaagctc
    11881 acgctggcgc aggccgagca gccgcaagcg gcgcttcggg ccgccgcggc acacgcgctg
    11941 cgcctgcacg aaagcgacct gggcgccctc caggtcttca aacgcagctt cgacgcgcgc
    12001 aaggccgaat tgacggcggt gtacatcgtg gacgtggcgc tggccgatcc cgcgcgcgaa
    12061 gcggcggtgc tcgctggatt tcccggccat ccccatatcg ggccgacacc cgacatggag
    12121 tggcatccac cggtgcaagt gcgcgaggcg ctttccgaac gcccggtggt cgtcggcttc
    12181 ggcccttgcg gcattttcgc ggccctggtg ctggcgcaga tggggctgcg gccgatcgtg
    12241 ctcgaacgcg gcaagaaggt gcgggagcgc acacgcgaca cctggggcct ttggcgcaag
    12301 cgggtgctcg acccggaaag caacgtgcag ttcggcgaag gcggcgccgg cactttctcc
    12361 gacggcaagc tgtacagcca gatcaaggac ccgcgccacc tgggccgcaa ggtgatgaac
    12421 gagttcgtca aggccggcgc gcccgaggag atcctttacg tcgcgcaccc gcacatcggc
    12481 accttcaagc tggtgaaggt cgtggaaaac atgcgcgaac agatcaccgc gctcggcggc
    12541 gagatccgct tccggcaccg cctgaccgac ctgctgatcg aggatggccc cgatggcaag
    12601 cgcttgcgcg gactcacggt gctggaccag gccagcggcg ccgtcgccga aatgcgcgcc
    12661 gagcatgtgg tgctggcgct gggccacagc gcgcgggaca cgttcgagat gctgcaccgg
    12721 cgcggcgtcc atatggaggc caagccgttc tcgatcggct ttcgcgtcga gcacccgcag
    12781 agcgtgatcg accgcgcccg ctggggccgc cacgccggcc atccgctgct gggtgcggcc
    12841 gactacaagc tggtgcacca cgcgaacaac ggccgctcgg tgtacagctt ctgcatgtgc
    12901 cccggcggca ccgtggtggc ggcgaccacg gaggcgcagc gcgtggtcac caacggcatg
    12961 agccagtatt cacgcaatga acgcaacgcg aacgcgggga tggtggtggg gatcgatccg
    13021 cgcgactatc cgggcggccc gctggcgggc gtcgagctgc agcgccaact cgaatcgaac
    13081 gccttcgtgc ttggcggcgg caactacaac gcgccgggcc agctggtcgg cgacttcatc
    13141 gccggccggc cctcgacgca gttcgggggg gtcgagccct cctacacacc cggcgtgacg
    13201 ctgggcgacc tgcatggcgc cttgcccgag tacgcgatcg ccgcactgcg cgaagccttg
    13261 cccgcgttcg ggcgcaagct caggggattc gacatgcccg atgcggtgtt gacgggtgtg
    13321 gaaacccgca cctcgtcgcc gctgaagatg acccggggcg aggattacca gagcgtgaac
    13381 gtgcgcggcc tgtacccggc cggcgaaggg gccagctacg ccggcgggat actttccgcc
    13441 ggcgtggacg ggatcaaggt cggggaggcg ctggcactcg atcttgtccc ctctcctgcc
    13501 aattcatccc ctctcccttt gggagagggt tagggtgagg gctggcgctt catgccctca
    13561 ccctcgccct ctcccgcagg gaggaggagc aatacatcac accgcggcat tctgcagcgc
    13621 cgcaatccgc tcctcgatcg ggggatgcgt ggagaacagc ctgcccacgc ccgaggtgat
    13681 gccgaaggcc tggatgctct tgggcagttc gcccgggacc atgccgccca ggcgggccag
    13741 cgcattgatc atcggctgct tgcggcccag caactgggcc gagccggcgt cggcgcgaaa
    13801 ctcgcgctgg cgcgagaacc aggccacgat gatcgccgcc aggaagccca gcaggatgtc
    13861 cagcacgatg gtgctcacga agtagccgat gccggggccc gatcgagagc tgccgcggct
    13921 caggaagctg tccacggcgt agctgatcac tcgcgacagg aacacgacga aggtgttcat
    13981 cacgccctgg atcagcgtca tcgtcaccat gtcgccattg gcgacgtggg cgacctcgtg
    14041 gccgatcacg gcctcgactt cctcgcgcgt catgccctgc agcaaaccgg tggagactgc
    14101 caccagggcg ctgttcttga acgcgccggt cgcgaacgca ttgggctcgc cctggaagat
    14161 gccgacttcg ggcatgccga tgccggcctg gttggcaaac ctgcggacgg tctcgacgat
    14221 ccacgcttca tcctggttgc gcggctgttc gatcacctgc acgccggcgc tccacttggc
    14281 caccggcttg gagatcagca acgaaatgat cgcgccgccg aagccgatga tcgccgagaa
    14341 gatcagcagc gaggtcaggt tcaggccctg cgccgtgaga tagcggttca cccccagcag
    14401 gctcgccacg atgcccagca ccaccacgac ggccaggttt gtgagcacga acagaacgat
    14461 acgtttcatg gaatctccga agaaattgac acaccaaccc gggcacagat gaggctcggc
    14521 gccgccaaat caagtgcgcg aagcggtgga cctcctggcc ggcgcccgct ggctcgcacg
    14581 cccggccggc cgaaatacga gggcgtcatc atagaaggaa aagtcctggt tgccggccca
    14641 ccagcccggt atgcccagca gtggcagggg cgtgaacggc ttttccgaca gccggccggc
    14701 cgtcagctgt tcggccagcc aacggtcggg cgagcccgcc accggcgcgg tgttggcgca
    14761 ccagacatgc gcggtgagat ccttccgggg gctcaccagt ttctccagca gcgcatggcc
    14821 gaacaccacc acagccgctt cgctccacag cgggcgcagc tgcacgaaca ggcgccgcca
    14881 atcgcgcgcc agcagcgcct cccacaacgg ttgcggcgcg gcgagcagcg ccccgttttc
    14941 atcgaacagg gtgagcgcgt cgcgcacggc gccgcgccgc ggaccgacgc cggcgcgcgc
    15001 gatctccccc gcttgcaact cgttgaggcg gcgcttggtc cgggtgaacc gcagccacgc
    15061 gaggccgttg aagaagtcgt ggagattgtc gcgagtggga acgctgccgg tgcgcgcaac
    15121 aaacgcttca tacgcctcgc ccggcgttaa attgaaagcg gggatgaact ccggaacggc
    15181 aacaccggac tcgcgcggtg cgagccccgc cagggccgcc gctgcgctcg aacccgcctg
    15241 gacggcttgc cgcccggcat ccagccaccc ggcataaggc gcgaaccacg ggtgccgcac
    15301 gtcgaccgcg gcgagttgct gcaggaacgc ggcactcacg ctggctggga cggcgtgaac
    15361 tgcaccttgc tcggccgctt gtccggactc agcacgaaca gctcgggatg cttcctgaac
    15421 agcttgaggg agctcgcgct cttgcccagc agtttgccgg cgcgcagcaa ttgcgccgcc
    15481 tggttcagtt ccaccctggc gccgccgcgc aactcgggca ccacgtccag aatgcgcagc
    15541 acttcttcgg gcacgctggg cgtggttggc tcagcgcgcg acctcgcgcg ccgtggcgca
    15601 cggccggccg gctggggcgc gggctccggt gcgggcgcgg gcgcacgcgc acgcgccggc
    15661 gcggacgtcc ttggtccgtg atgctggatg tcggtgaacg cgtcgtacac ggccgtggtc
    15721 tcatcgccgg tcttgccccg ttggccgatg ccgcagacgc ggcagccttt ttcgcgcaac
    15781 cggatcacca gcggcgcgaa atcggagtcg gacgacacca gcaccaccac gtgcggccgc
    15841 tcggcgatca cgaggtccat ggcatcgacc gccagcgcga tgtcggtgct gttcttgccg
    15901 gcggagaggt tcaccaacgg ccgcaccagc agcctcttga acagcgcctg ctgcttcacc
    15961 gccgtttcgg cgttgcagta ggcccgccgc acatggatgg cgccgtgctc cgccagtgtg
    16021 cgctggatgg cttgttcgat gacgtcggcc gagacgttgt cggcatcgat cagcaaggcg
    16081 acgcgttggg attcgttcat gccagcctcc agctcagggg ttcgccgcca cgcaggggct
    16141 tgagcgtcgc ctcgccgaag gcaaagcttt cgggcggctt ccacgtttcg cggcgcaggg
    16201 tgatcttttc ccggttgcgc ggcaggccgt agaaatcggc gccgtgcaag ctcgcaaagc
    16261 cttccagccg gtccagcgcg ttcgcatcgt cgaaggcctc ggcataaagc tccatcgccg
    16321 cgtgcgccgt gtagcagccg gcgcagcccg tggcgtgttc cttcaggtgc gccgggtgcg
    16381 gcgcgctgtc ggtgcccagg aagtacttcg ggttgccgcc ggtggccgct tcgaccagcg
    16441 cctggcggtg cgtttcgcgc ttgagcaccg gcaggcagta gtaatgcggc cggatgccgc
    16501 cggtgaagat cgcgttgcgg ttgtacagca ggtggtgggc ggtgatcgtg gcggccacga
    16561 accggtccga gtcgcgtaca tactgcgccg cttcgcgcgt ggtgatgtgc tcgatcacca
    16621 ccttcaactc cggaaagtcg cggcgcagcg ggatcagccg ggtgtcgagg aacaccgcct
    16681 cgcggtcgaa aacgtccacg gccgggtccg tcacctcgcc gtgcaccagc agcggcaggc
    16741 cctcccgctg catcgcttcg agtgtcctgt aggcgttgcg caggtcggtc acgccggcgt
    16801 cgctgttggt cgtggctccg gcggggtaaa gcttgacggc gacgactcca atttcctttg
    16861 cgcgcacgat ctcgtcgggc gggagcctgt ccgtgaggta cagcgtcatc aagggctgga
    16921 agctcatgcc gggcggcacc gcctcccgga tgcgctcgcg ataagccatc gcctgggcgg
    16981 cggtggcgac cggtggccgg aggttaggca tgatgatcgc gcggccgaac tgggcggccg
    17041 tgtgcggcac caccgtcttc agggcttccc cgtcgcgcac gtgcacgtgc cagtcgtccg
    17101 gccgggtgat cgtgatttcc tgggtcatga cgctattgtc gccggaccct tcggggtagc
    17161 cgcggaaccg gctttgccgg gccgctggct gcgcccccca gtgaggggga gagggcgaag
    17221 cccgaggggg catcggcgca gccgcttcgg ggggcgtcct tcaatgcgcc aggatcttgt
    17281 tcaggaaatc cttcgtccgc ggctggcggt tctcggggtg accgaagaac tcgtccgtgg
    17341 tgcagtcttc caggatcttg ccgccgacgt cgatgaagat cacgcggctg gctaccttgc
    17401 gggcgaaacc catctcatga gtgaccacca tcatggtcat gccttccttg gccagcgcca
    17461 ccatcacgtc cagcacctcg cccaccatct ccgggtccag cgccgaggtc ggctcgtcga
    17521 acagcatcac gatcgggtcc atcgacagtg cgcgcgcgat cgccacgcgc tgctgctggc
    17581 cgcccgacag ctggcccggg aacttgtcct tgtgcgccat cagccccacc cggtcgagca
    17641 ttttcagccc gcgcgcgagc gcctcgtcgg ggctgcggtt cagcaccttg atctgggcga
    17701 tggtcaggtt ctcggtcacc gaaagatgcg ggaacagctc gaaactctgg aacaccatgc
    17761 ccacccggct gcgcagtttg ggcagatcgg ttttcgggtc gtgcagcgga atgccttcca
    17821 cgaagatctc gcctttctgg aacggctcga gcgcgttcac ggtcttgatc agcgtcgatt
    17881 tgccggagcc cgaaggcccg cagaccacca ccacatcacc cttgttgatg ttcaccgagc
    17941 agtcggtgag cacctgcacc ggaccatacc acttggagat gttcttgatt tcgatcatgg
    18001 cactcattgg ttttctccag attcaattgc tgccgaaacg ccccaggtca tggagctgcg
    18061 gccatagtcc aaggggccac cgcggaaccg gctttgccgg gccgctggtg ggccttgcag
    18121 gccgcctggc cgcaagacgc ggccattcct cgacctgtcc aaaccgacgc aggtcgg
//
LOCUS       DASWIY010000039         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1876874, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000039 DASWIY010000000
VERSION     DASWIY010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876874"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VGA59_01470"
     CDS             complement(<1..1200)
                     /locus_tag="VGA59_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX9718373.1"
                     /translation="MAHANRENMANAIRALAMDAVQQANSGHPGAPMGMADIAVALWG
                     RHLKHNPGNPHWFDRDRFVLSNGHASMLIYALLHLTGYELPIGELKNFRQLHSKTAGH
                     PEVGVTPGVETTTGPLGQGIANAVGMALAEKLLATEFNRDGHTIVDHHTYVFMGDGCM
                     MEGISHEACALAGAWKLGKLIAVYDDNGISIDGEVAPWFVDDTPARFKAYGWNVIGPV
                     DGHDIQAVERAVSDARRGVDKPTLIVADTHIGRGSPNRANTAKAHGEALGLEEVKLTR
                     EAMGWPHEPFVVPREVYADWDARAQGAAAQAEWDKRFAAYGAAFPELAAEFSRRMKGE
                     LPKNFAQVTVDAAVAAHAKAETVASRKASQLALEAFTAALPEMIGGSADLTGSNLTNT
                     KSTPPLRF"
     gene            1387..1767
                     /locus_tag="VGA59_01475"
     CDS             1387..1767
                     /locus_tag="VGA59_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEX9718374.1"
                     /translation="MQGLHLTADLYQCRCDAAWLIDAQRLCDWCLEAVRAVGLQPVNQ
                     LFHSFPAAGDGAAGGVTAALLLAESHVCLHTWPEKRGVTLDVYVCNFGGDHSAKARGL
                     MFALINRFQPEWTEQRSLDRGDGE"
     gene            1984..>2206
                     /locus_tag="VGA59_01480"
     CDS             1984..>2206
                     /locus_tag="VGA59_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9718375.1"
                     /translation="MTMGSSEAMILAAGRGERMRPLTDQCPKPLLQVRGKPLIQYHTE
                     ALARAGFRELVVNTAWLGHQIENHLSGPAA"
BASE COUNT          340 a          727 c          750 g          389 t
ORIGIN      
        1 gaagcgcaac gggggcgtgc tcttggtatt ggtgaggttg gaaccggtca ggtcggccga
       61 gccgccgatc atttcgggca gggccgcggt gaacgcttcc agcgccagtt gcgaggcctt
      121 gcgcgaagcc acggtctcgg ccttggcatg cgcagccaca gctgcgtcca ccgtcacctg
      181 tgcgaagttc ttcggcagct cgcctttcat gcgacggctg aattcggcgg ccagctccgg
      241 aaaggcggca ccgtaggcgg cgaagcgctt gtcccactcc gcctgcgcgg ccgcgccctg
      301 ggccctcgcg tcccaatcgg catacacctc cctgggcacc acgaaaggct catgcggcca
      361 gcccatggcc tcgcgcgtga gcttgacctc ttccaggccg agcgcctcgc cgtgcgcctt
      421 ggcggtgttg gcgcgattcg ggctgcccct gccgatatgg gtgtccgcca cgatcagcgt
      481 gggcttgtcg acaccgcggc gtgcatcgga gaccgcgcgc tcgaccgcct ggatgtcgtg
      541 gccgtcgacc ggcccgatca cgttccagcc atacgccttg aagcgcgccg gcgtgtcgtc
      601 gacgaaccag ggcgccactt cgccatcgat cgagatgccg ttgtcgtcgt acacggcgat
      661 cagcttgccc agcttccagg cgcccgccaa ggcacaggct tcgtggctga tgccttccat
      721 catgcagccg tcgcccatga acacataggt gtggtggtcg acgatggtgt ggccgtcgcg
      781 gttgaactcg gtggccagca gcttttcggc cagtgccatg ccgacggcgt ttgcgatccc
      841 ctggcccagc ggtccggtgg tggtttcgac gccgggtgtg acaccgacct ccgggtgccc
      901 ggccgtcttg ctgtgcagct ggcggaaatt cttcagctcg ccgatcggca gctcgtagcc
      961 cgtgagatgc aacagcgcgt aaatcaacat cgaggcgtgg ccgttcgaga gcacgaaacg
     1021 gtcgcggtcg aaccagtgcg gattgcccgg gttgtgcttg agatgccgtc cccacagcgc
     1081 gacggcgata tcggccatgc ccatgggcgc accgggatgt ccggagttgg cttgctgaac
     1141 ggcatccatc gccagggccc ggatggcgtt cgccatgttc tcgcggttgg cgtgtgccat
     1201 aggtagatcg ttgaccatcg ggggcagctc cggggggtgg tatcagggga aacccgagat
     1261 tttacccgcc ggcacggcag ggcccggccg gccgcgcccg catcgaaacg gcaggacctt
     1321 cggcggcggc cccgaccggc tcctttttga cgcgcttcag cgcgatgatc gtctacagtc
     1381 atggccatgc aagggctcca tctcactgcc gacctgtacc agtgccgttg cgatgcggcg
     1441 tggctgatcg acgcgcagcg cttgtgcgat tggtgcctgg aggcggtgcg ggctgtcggc
     1501 ctgcagcccg tcaaccagct cttccactct ttcccggccg ccggcgacgg cgcggcgggt
     1561 ggtgtgacgg ccgcgctgct gctggccgag tcgcatgtct gcctgcacac ctggcccgaa
     1621 aaaaggggcg tgacgctgga cgtctacgtc tgcaatttcg gcggcgacca ttccgccaag
     1681 gcgcgcggcc tgatgttcgc cctgatcaac cggttccagc cggaatggac cgaacagcgg
     1741 tctttagacc ggggcgatgg agagtgaaaa cacgaagggc gcgggccccg cttgcggggt
     1801 cgaccagagt tttcactctc gagggggccg ggctcgatgc ccggctcgcg aagcaaacgg
     1861 tcgaattact gccgctctac gcacgaacgc gcccccgcat cgcggggtcg accagagttt
     1921 ccactctcga gggggccggg ctcgatgccc ggcccgcgaa gcaaacggta gcggagacac
     1981 ccgatgacca tgggtagctc cgaagccatg atcctggcgg ccgggcgggg cgagcgcatg
     2041 cggcccttga ccgaccagtg ccccaagccg ctgctgcagg tgcgcggcaa accgctgatc
     2101 cagtaccaca ccgaagctct ggctcgcgcg ggttttcgcg agctggtggt gaacacggcc
     2161 tggctggggc accagatcga aaatcatttg tccggcccgg cggcgc
//
LOCUS       DASWIY010000040        12151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_398226, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000040 DASWIY010000000
VERSION     DASWIY010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 12151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12151
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398226"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1828
                     /locus_tag="VGA59_01485"
     CDS             <1..1828
                     /locus_tag="VGA59_01485"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEX9718376.1"
                     /translation="RFAHLEHAITAQAGDTLFQSARRNGVRIVGACGGRGTCGTCAIH
                     VVEGQVERSPHAAPSREPGETRSGRKKWQRACQLRALSDCTVEVAPRSLARVVRADVA
                     SSTDVEPLPLDPLVRSLDLSVAPASLADPASDADRLRRALPGQDLRLDLGALRQLPGV
                     LRAGSWSLRAHLRRSELIGVAPAGSASLGLAVDLGTTNVAAFLIDLQSGQRLASLAIE
                     NPQVAWGADVISRVNHAVTHPGGGDELRDAAIIVLDALAHDLCQAVAAAPTDIVDVVV
                     CGNTAMQHLLLGLPVSQLGRAPFVAAVRQGMDVKARELGLAVCPGAYVHVAPAVGGFV
                     GGDHVTALLATQHLWAGDKTSLVMDIGTNTEISLIHQGQILSASCPSGPALEGGHISC
                     GMRAAEGAIERVGLAADGSLTLKVIGKKTPVGLCGSGVLDVMAALRRAGMVDGRGRLD
                     AGHPAVGERDGERVAELAEGVSFSQDDVRAVQLAKSAVRTGVELLLRQAGLEAGDIER
                     FIIAGAFGAYIAVQSGIDTGLFPALAPVRFVQVGNAAGLGVQRMLASGRARDEAGELA
                     ARCRYVELSTLAGFQKTFLQHIAFKCHPGLDPGSMDCGSSPQ"
     gene            1858..2745
                     /locus_tag="VGA59_01490"
     CDS             1858..2745
                     /locus_tag="VGA59_01490"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9718377.1"
                     /translation="MTTPQSFNITIIGERINPGFKSTKALFDNTDLPGIQALAVKQAE
                     AGASYLNVNIGARALTDAVFLTEAIQAIQAVVTLPLSFDVPSLQAHELCLQTYDQERA
                     GGALPIINSITEHRWGAMSLYKQRPFKVIVMASERVEDGVARNNKSAADIAGTAKRAA
                     LRLREDYGMALDDVIIDLSVSAIIADTEGLNRGTLEAVKLIGSDPELKGLHMMGGLSN
                     IGQQLPPKAADGSDLKHDLENAFLTLAVPHGFDTVLGTPWRGYAPLAEDNYVLKAYQN
                     FLEQTGSNALRAVRKLYKA"
     gene            2742..3956
                     /locus_tag="VGA59_01495"
     CDS             2742..3956
                     /locus_tag="VGA59_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9718378.1"
                     /translation="MNSPNSFALVVDEWFDGDERHPGRSTMKIVDGRLADVAAGDHAA
                     ALTGQGWPVERVAFLMPGLVDAHVHLFLDGATTDSKLRSEHLKQPLNALTEAGRRAAA
                     QALIHGVTTVRDGGDRHGINHRLRAEARRPGSRLAQVRSVGLGVKRAKRYGAFMATDV
                     GDDESIRGSVRDLARANDEIKLILTGIIDFDAGAVTDEPQFSLDEARSVVETAHAAGR
                     KVMTHCSGLKGLAVAAGAGVDSIEHGFFMDRATLALMRDKDLAWTPTFCPVHFQWARP
                     EAAGWSAQTVGHLRRILDAHAGHLLLAHQMGVRLLLGTDAGSMGVKHGVAVFEEIDRF
                     LEAGLPLAAVLRAATSQPRRHFADPHPVLVAGAVFEAVQFAASPFADAQVLRRPQRIW
                     APSMRAAQPSGH"
     gene            4048..5406
                     /locus_tag="VGA59_01500"
     CDS             4048..5406
                     /locus_tag="VGA59_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9718379.1"
                     /translation="MLAPLVRWLGSRRAEAVLHPFERAAKRFMFDCRECGQCVLSATG
                     MACPMNCPKQMRNGPCGGVRADGTCEVDPQMRCVWVEATEGTKRIAADHLAHPTPLLP
                     AIDTRNAGRSTWIRVIEGHPETAFQPPVGERRTAQSRLGPFETACRSGDFVVTVEVTP
                     PDSADPHALLERAARFSGLVDAINITDGAGGNCHMSSVAASAVLVAHGLPAVYQITCR
                     DRNRIAIQGDILGAAALGVQNILCLTGDDVSQGDHPQAKPVFDLDAVSLLHIARGMCD
                     QGQYASGRKLETPPDMFIGATANPFVPPYRDRVANLEKKIVAGARFIQTQFCFDVEMF
                     EDFMQEVRQRELQHHAKIIVGVGTLGSAKALAWMAAHVPGVHVPRGLLERIGGAKDQR
                     LEGKRVCIETIRALTRIEGVAGVHVMGHRNEDVLAEIIVESGIGRGRRADRRDIDSSA
                     SA"
     gene            complement(5450..6217)
                     /locus_tag="VGA59_01505"
     CDS             complement(5450..6217)
                     /locus_tag="VGA59_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9718380.1"
                     /translation="MTQSTSSKPAETAEAGASQAVKAQLRLREMILAGELPSGARIAE
                     LAIVEKLGVSRTPIRAALVRLEQEGLLQALPNGGFAVRTFSERDASDAIEMRGTVEGL
                     AARLAAERGAPGVVLAQARACLAQIDAVLSQPALDDEAFLRYVTLNQKFHNLLSEMAD
                     SAVIRRELERVVSLPFASPSAFVVMQANSPQARDMLVVAQDQHRQVLDAIDRREGARA
                     EAIMREHSRIAQRNLREALQGHDLDRMPGARLIRKRG"
     gene            6435..7478
                     /locus_tag="VGA59_01510"
     CDS             6435..7478
                     /locus_tag="VGA59_01510"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX9718381.1"
                     /translation="MFPKNTWYVACTPAEIDDKPLGRRICGEKIVFYRADEGKVAALE
                     DFCPHRGAQLSLGQVCEGKLVCGYHGLAMGCDGKTVSMPGQRVRGFPQIRSYPVMERY
                     GFVWVWPGEATLADASKIGHLEWADNPEWAYAGGLYHIHCDYRLMIDNLMDLTHETYV
                     HTTSIGQKEIDQVPVTTRVEGDTVVTSRFMENVMPPPFWSANLRGNNLADDVPVDRWQ
                     VCRFTPPSQVMIEVGVAHAGKGGYDADSKHKVHAIVVDFLTPETETSMWYFWGMARNF
                     NPRDKALTAQIRDGQGKVFAEDLAVLEAQQANLARYPDRKLLALNIDAGGVQSRRVID
                     RLIAQEQPQLKAA"
     gene            7478..8434
                     /locus_tag="VGA59_01515"
     CDS             7478..8434
                     /locus_tag="VGA59_01515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEX9718382.1"
                     /translation="MESLAVQVVRKAREAQDIVSFELARVGGAPLPAFSAGSHIDVQV
                     PGGLTRQYSLCNDSGEQHRYRIAVLRDAASRGGSVAMHDAVHEGDVIHISEPRNHFPL
                     HHAARSLLFAGGIGVTPLLCMAQRLATIGADFALHYCTRSPERTSFLDEIAASPFAGR
                     VQFHFDSGPPGQRLDLQALLASPDAGTRIYVCGPTGFIDHVIGSAKARGWNSEQIHLE
                     YFGAAPQDTSGDTAFDVKIASTGKTYQVAAGQSVVHALREHDVEILTSCEQGVCGTCI
                     TRVLEGEPDHRDMYFTEEERSKNDQFTPCCSRARSKALVLDL"
     gene            8523..9635
                     /locus_tag="VGA59_01520"
     CDS             8523..9635
                     /locus_tag="VGA59_01520"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX9718383.1"
                     /translation="MTPLPARYDHVGSFLRPKSLLEARERKAKGEITPEQLRQVEDKA
                     IGEIVKFQEDVGLKSITDGEFRRTYFHIDFLEQLGGVKTDIPVTIKKPDGTEELAPPV
                     MRVIDKVRHAKNIQLADFQYLKSQVSAGHTPKVCIPSPTMLHFRGGRAGISKEAYPEL
                     EPVFYDDVAKAYGDELRSLAAAGCTYVQLDDTNMAYLCDEKMREAARQRGDDPNELPH
                     RYAKFINKVVAQKPKGMLLAMHLCRGNFKSTHAATGNYEPVAEALLKEMDLDAYFMEY
                     DDDRSGDFRPLRYLPKGKTVVLGLVTTKFGELENKETLKRRIAEAAQYAPLEQLALSP
                     QCGFSSTVHGNKIAVEAQRSKLRLVIETAQEVWGEN"
     gene            9690..10652
                     /locus_tag="VGA59_01525"
     CDS             9690..10652
                     /locus_tag="VGA59_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEX9718384.1"
                     /translation="MAALGTASLPLAAWRPARSQPGRMQSWPLGTPRRTGIHDVAPAP
                     DGGVWLTAQASGHLGWFDPKAGRTELIALGSGSSPHGVIQGPDKAAWITDSGQNAIVR
                     VGWPDRKVQAFALPAAASRANLNTAAFDGDGDLWFTGQGGYVGKVALKTGQVSVKDAP
                     RGRGPYGICSTPGGDVWWCSLGGSFIARIDRRTGNSSVVEPPTRNQGARRVWSDSKGR
                     IWVSEWNSGNLSMHDPQAGTWRQWKVPGGNPQPYAVYVDERDVAWVSDFGGNAVFSFD
                     PRTEKFDRYGFPRDSANVRQIHGRPGEVWLPESGTEHITVIRKG"
     gene            complement(10667..11104)
                     /locus_tag="VGA59_01530"
     CDS             complement(10667..11104)
                     /locus_tag="VGA59_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718385.1"
                     /translation="MGVLLLCAASLASYAGNSEISRRTQEAGPTGGTLSVKNLVDALR
                     AAGGTVQSEGSVDQPFFAVPAKTINVDGEGVQVFEYPRAAAADAQAATVSRDGSQVGT
                     SKPFWIGAPHFYKKANLLVLYLGDDEKVLKLLQSVLGQQFAGR"
     gene            complement(11171..12040)
                     /locus_tag="VGA59_01535"
     CDS             complement(11171..12040)
                     /locus_tag="VGA59_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718386.1"
                     /translation="MNPASRFHSHAIRSIAIYAAVLIGATVSVSALANHSWGGYHWAR
                     TTTPFTLQLGNNLTSVDWRFSFHTASEDWNSPPESSTPIWTLAVTGGANRRCSMVKGT
                     TQVCNGKYGKNGWLGLATIYISGDHITQGSAKMNDTYLSAATKYNNPNERQHVMCQEI
                     AHTFGLDHQSEDGKSLNTCMDYFSNTGANATSTLSISPNTHDFEQLNFIYDEHLDSTT
                     TVASSAASAASTTPDLSDDPISWGTLMRQSADGRSSIYERYNRDGTHTLTHVYWTEET
                     ASACPSCDHRVYR"
BASE COUNT         2135 a         4011 c         3984 g         2021 t
ORIGIN      
        1 acgctttgcg catctggaac atgccattac cgcgcaggcc ggcgacaccc tgttccagag
       61 cgcacggcgc aacggggtgc ggattgtcgg tgcctgcggc ggacgcggca cctgcggcac
      121 gtgcgcgatc catgtcgtcg aaggccaggt cgagcgcagc ccgcacgcgg cgcccagccg
      181 cgagcccggc gagacgcgca gcgggcgcaa gaagtggcaa cgcgcctgtc agctgcgtgc
      241 cttgtccgac tgcacggtcg aagtcgcgcc gcgttcgctg gcaagggtgg tgcgcgccga
      301 cgtagcgagc agcaccgacg tcgagccgct gccgctggac ccgctggtga ggagcctgga
      361 cctgagcgtg gccccggcca gcctggccga tcccgcttct gatgccgacc ggctgcgccg
      421 cgccctgccc ggccaggatc tgcgcctgga tctgggcgcg ctgcggcaac tgcccggcgt
      481 gctgcgcgcg ggttcatggt cgctgcgcgc gcacctgcga cgaagtgaac tgatcggtgt
      541 ggcccccgcc gggagcgcca gcctgggcct ggcggtggac ctgggcacca ccaatgtggc
      601 ggccttcctg atcgatctgc aaagcggcca acgcctggcc agcctcgcca tcgagaaccc
      661 gcaagtggcc tggggcgcgg acgtgatcag ccgggtcaac cacgccgtca cccatcccgg
      721 cggcggcgac gaattgcgcg atgccgcgat catcgtcctc gatgcactgg cccacgacct
      781 gtgccaggcc gtggccgccg cgccaacgga catcgtggac gtggtggtct gcggcaacac
      841 ggcgatgcag catctgctgc tgggcctgcc ggtgtcccag ctcggccgcg cgccgttcgt
      901 cgcagcggtg cgccagggga tggacgtcaa ggcgcgcgag ctcggcctgg cggtctgccc
      961 gggcgcctat gtgcatgtcg cgcccgccgt cggcggcttt gtcggcggtg accatgtcac
     1021 ggccttgctg gccacgcaac acctctgggc cggcgacaag acgagcctgg tcatggatat
     1081 cggcaccaac accgagatct ccctgattca ccaggggcag atcctcagcg cctcctgtcc
     1141 ctcgggaccc gcgctggagg gcggccacat ctcctgcggc atgcgcgcgg ccgaaggcgc
     1201 catcgagcgc gtggggctgg cggcggacgg ctcgctcacg ctcaaggtga tcggcaagaa
     1261 gacacccgtg ggcctgtgcg gctccggcgt cctggacgtg atggccgcct tgcgccgcgc
     1321 ggggatggtc gatggccgcg gccgcctcga cgcaggccat cccgccgtgg gagagcgcga
     1381 cggcgagagg gtggccgagc tggccgaagg cgtgagtttc agccaggacg atgtgcgcgc
     1441 cgtgcaactg gccaagtcgg cggtccgcac cggcgtcgag ttgctgctgc gccaggctgg
     1501 attggaagcc ggcgacatcg aacggttcat catcgccggc gctttcggcg cctacatagc
     1561 cgtccaaagc gggatcgata ccggcttgtt cccggcgctg gcgccggtgc gctttgtcca
     1621 ggtcggcaac gccgcgggcc tgggtgtgca gcgcatgctg gcctcgggcc gggcgcggga
     1681 cgaagccggt gaactcgccg cgcgctgccg ctacgtcgag ctcagcaccc tggccgggtt
     1741 ccagaagact ttcctgcaac acatcgcctt caaatgtcat cccggacttg atccgggatc
     1801 catggattgc gggtcaagcc cgcaatgaca gaactcaaat tccgtaagga atacaccgtg
     1861 accacacccc agagtttcaa catcacgatc atcggcgagc gcatcaatcc gggcttcaag
     1921 agcacgaagg ccttgttcga caacacggac ctgccgggca tccaggcctt ggccgtcaaa
     1981 caggccgagg ccggggcgtc ttatctgaac gtcaacatcg gcgcccgtgc gctgaccgac
     2041 gcggtctttc tcaccgaagc catccaggcg atccaggccg tggtcacgct gccactgtcg
     2101 ttcgacgttc ccagcctgca agcccacgaa ctgtgcctgc agacctatga ccaggagcgc
     2161 gccggcgggg cccttcccat catcaattcg atcaccgagc accgctgggg cgcgatgagc
     2221 ctgtacaagc agcggccatt caaggtgatc gtgatggcgt ccgagcgggt cgaggacggc
     2281 gtggccagga acaacaagag cgcggccgac atcgcgggca cggccaaacg cgccgcgctg
     2341 cgcctgcgcg aggactacgg gatggctctg gacgacgtca tcatcgacct gtcggtgtcg
     2401 gccatcatcg ccgacacgga ggggctgaat cgcggcaccc tggaagcggt caagctgatc
     2461 ggcagcgatc ccgagttgaa ggggctgcac atgatgggtg gcctctcgaa tatcggccag
     2521 caattgccgc cgaaagccgc tgacggctcg gacctgaagc acgacctgga aaacgccttt
     2581 ctcacgctgg ccgtgccgca cggcttcgat accgtgctgg gcacgccctg gcgcggctac
     2641 gccccgctgg ccgaggacaa ctacgtgctc aaggcgtacc agaacttcct cgaacagacc
     2701 ggcagcaacg ccctgcgcgc ggtgcgcaag ttgtacaagg catgaactcg ccaaactctt
     2761 tcgcgctggt cgtcgacgaa tggttcgacg gcgacgagcg gcacccgggc cgcagcacga
     2821 tgaagatcgt ggacggccgg ctggcggatg tggcggccgg cgaccacgcc gctgcgctga
     2881 ccgggcaggg atggccggtg gagcgcgtcg cgttcctgat gccggggctg gtggatgcgc
     2941 atgtccacct gttcctcgac ggggcaacga cggattcgaa attgcgctcg gagcacctga
     3001 agcagccgct caacgcgctg actgaagcgg ggcgccgcgc cgcggcccag gcgctcatcc
     3061 atggcgtgac cacggtgcgc gacggcggcg accgccacgg catcaaccac cggctgcgcg
     3121 ctgaagcacg ccgtcccggc agcaggctgg cgcaggtgcg ctcggtcggc ctgggcgtca
     3181 agcgcgccaa acgctacggc gccttcatgg ccaccgatgt gggcgacgac gagagcatcc
     3241 gcggctcggt gcgtgacctg gctcgtgcca acgacgagat caagctgatc ctcaccggca
     3301 tcatcgactt cgatgccggc gcggtgaccg acgagcccca gttcagcctc gacgaggcgc
     3361 gcagcgtggt cgagaccgcg cacgcggctg gccgcaaggt gatgacccat tgcagcggcc
     3421 tcaagggcct cgcagtggcg gcgggcgccg gtgtcgattc gatcgagcac ggctttttca
     3481 tggatcgcgc cacgctggcg ctgatgcgcg acaaggacct cgcctggacg cccacgttct
     3541 gcccggtgca tttccagtgg gcccggcccg aggcggcggg ctggtcggcg cagaccgtgg
     3601 gtcatctgcg ccgcatcctc gatgcgcacg ccggacatct cttgctggca caccaaatgg
     3661 gcgtgcgcct gctgctgggc accgacgccg gcagcatggg agtgaagcat ggcgtggcgg
     3721 tgttcgagga gatcgaccgc tttctggaag cgggactgcc gttggcggcc gtgctgcgcg
     3781 cagccacttc ccagccgcgt cgccacttcg ccgatccgca cccggtgttg gtggcaggtg
     3841 cggttttcga agccgtgcaa tttgctgcat cacccttcgc cgatgcacaa gtgctgcgcc
     3901 ggccgcagcg aatctgggcg ccttcgatgc gcgccgcgca accgtcgggc cactgaaagc
     3961 acgcccatgt acagcgtccg cctctggtcc gtccggcatg cacgcggctt gcgccgcttg
     4021 tatgtgttct tctcgaggct cgcgcccatg ttggctccct tggtgcgatg gctgggcagc
     4081 cgccgtgccg aagcggtgtt gcacccgttc gagcgcgcgg ccaaacgctt catgttcgac
     4141 tgccgcgaat gtggccagtg cgtgctgtcg gccaccggta tggcctgccc catgaactgc
     4201 cccaagcaga tgcgcaacgg tccgtgcggt ggcgtgcgag ccgacggaac ttgcgaggtc
     4261 gacccgcaga tgcgctgcgt ctgggtcgaa gccaccgaag gcaccaaacg catcgccgcc
     4321 gaccacctgg cacacccgac gccgctgctg cctgccatcg atacccgcaa tgccggccgc
     4381 tccacctgga tccgggtcat cgagggccat cccgaaacgg cgtttcagcc gccggttggc
     4441 gaacgccgga cggcgcaatc gcggctcggt cctttcgaga cggcctgccg ctcgggggac
     4501 ttcgtcgtta cggtcgaggt gaccccaccc gactcggccg acccacacgc cctgctggaa
     4561 cgggccgcgc gattcagcgg cctggtcgac gccatcaaca tcaccgatgg cgcgggcggc
     4621 aactgccaca tgtcgagtgt ggcggcgtcg gccgttctgg tcgcccacgg cttgcccgcc
     4681 gtctaccaga tcacctgccg cgaccgcaac cgcatcgcca tccagggcga catcctcggt
     4741 gccgccgccc tgggcgtgca gaacatcttg tgtctcaccg gggatgacgt cagccagggc
     4801 gaccaccccc aggccaagcc ggtgttcgac ctcgatgccg tctcgctgct gcatattgcc
     4861 cgcggcatgt gcgaccaggg gcagtacgcg tcgggccgca agctggaaac gccgcccgac
     4921 atgttcatcg gagccacggc caatccgttt gtgccgccct accgcgatcg cgtggccaac
     4981 ctggagaaga agatcgttgc aggcgcgcga ttcatccaga cgcagttctg cttcgatgtc
     5041 gagatgttcg aagatttcat gcaggaggtg cgacagcgag agctgcagca ccacgccaag
     5101 atcattgtcg gcgtcggcac cttaggcagc gccaaggcgc tggcgtggat ggcagcgcat
     5161 gtgccgggcg tgcatgtgcc gcgcggcctg ctcgagcgca taggtggcgc caaggaccag
     5221 cgcctcgaag gcaagcgcgt ttgcatcgag accatccggg cgctcacccg gatcgaaggc
     5281 gttgccggcg ttcatgtgat gggtcatcga aacgaggacg tgctggcgga gatcatcgtt
     5341 gaatcgggga tagggcgtgg gaggcgcgct gaccggcggg acatcgattc gtcggcatcg
     5401 gcgtgatcgg gggcaacgag cgcctgtgct actcccgccg gccgcgcgtt tacccgcgct
     5461 tgcgaatcag ccgcgcgccg ggcatgcggt ccaggtcgtg gccttgcagc gcctcgcgca
     5521 ggttgcgctg ggcgatgcgg gagtgctcgc gcatgatggc ttcggcccgg gcgccctcgc
     5581 gtcggtcgat ggcgtccagc acctgccggt gctggtcctg cgccacgacg agcatgtcgc
     5641 gcgcctgcgg tgaattggcc tgcatcacga cgaaggccga gggcgaggcg aacggaaggc
     5701 tcaccacccg ctcgagttcg cggcggatca cggcgctgtc cgccatctcg ctgagcaggt
     5761 tatggaattt ctggttgagg gtgacatagc gcaggaaggc ttcgtcgtcg agcgcgggct
     5821 ggctcagcac ggcgtcgatc tgcgccaggc aggcgcgcgc ttgcgccaac acgactcccg
     5881 gtgcgccccg ctccgccgcc agccgcgctg ccagcccttc gaccgtgccg cgcatctcga
     5941 tcgcgtccga tgcatcgcgt tcggagaatg tgcgcaccgc gaagccgccg ttgggcagcg
     6001 cttgcagcaa gccctcctgc tccagccgca ccagggccgc ccggatgggg gtgcgcgaaa
     6061 cgcccagttt ttccacgatc gcgagctccg cgatgcgcgc cccgctcggc agttcgccgg
     6121 ccaggatcat ctcgcgcagg cgcaattgcg ccttgaccgc ctgcgaggcg cccgcctcgg
     6181 ccgtttcagc aggtttggaa gatgtcgatt gagtcatcat tgctttttat tgtatacacc
     6241 agtttgagaa caattgagta tttatggctc tatttcatct gcagtctcgc taaatcaggc
     6301 ttttataagc cctggaagcc gataaatttt ctgttagtgt atacattgat tggatttttt
     6361 ccgcataatt gccgtccgaa tcccggatcc gattggctcc ggcatcaccg aagttttccc
     6421 tcttcaggag caacatgttc cccaaaaaca cctggtacgt ggcgtgcaca cccgccgaaa
     6481 tcgacgacaa gccgttgggc cggcgcatct gcggcgagaa gatcgtgttc taccgcgctg
     6541 acgaaggcaa ggtcgccgcg ctggaagatt tctgcccgca tcgtggcgcg caactgtcgc
     6601 tgggacaggt gtgcgaaggc aagctcgtgt gtggctatca cggcctcgcg atgggctgcg
     6661 acggcaagac tgtctcgatg ccaggtcagc gcgtgcgggg ctttccgcag atccgcagtt
     6721 atcccgtgat ggaacgctac ggtttcgtct gggtctggcc gggcgaggca acactggccg
     6781 acgcgagcaa gatcggccac ctcgaatggg ccgacaaccc cgaatgggcc tacgcgggcg
     6841 ggctgtacca catccactgc gactaccgcc tgatgatcga caacctcatg gacctgaccc
     6901 atgagaccta tgtccacacc acgagcatcg gccagaagga aatcgaccag gtcccggtca
     6961 ccacccgcgt cgaaggcgac acggtggtca ccagccgctt catggaaaac gtgatgcctc
     7021 cgcccttctg gagcgccaat ctgcgcggca acaacctggc cgacgacgta cccgtggacc
     7081 gctggcaggt gtgccgcttc acgccaccca gccaggtcat gatcgaagtc ggcgtggcgc
     7141 acgccggcaa gggcggctac gacgccgatt cgaagcacaa ggtccacgcc atcgtggtgg
     7201 actttctcac gcccgaaacc gaaacctcga tgtggtactt ctggggcatg gcccgcaact
     7261 tcaatcccag ggacaaggcg ctcacggccc agatccgcga cggccagggc aaggtgttcg
     7321 ccgaggacct cgccgtactg gaagcccagc aggcgaacct cgcccggtac ccggatcgca
     7381 agctcctggc cctcaacatc gatgccggtg gcgtgcagtc gcgccgcgtc atcgaccggc
     7441 tgattgccca ggagcagcca caactgaagg cggcgtgatg gaaagcctgg ccgttcaggt
     7501 cgtgcgcaaa gcgcgggaag cgcaggacat cgtcagcttc gaactggcgc gcgtgggagg
     7561 cgcgccgttg ccggcgttca gcgccggctc gcacatcgac gtgcaggtgc ccggcgggct
     7621 gacgcgccag tattcgctgt gcaacgactc gggcgagcag catcgctacc gcatcgccgt
     7681 gctgcgcgat gccgcctcgc gcgggggttc cgtcgccatg cacgacgcgg tgcatgaagg
     7741 cgacgtcatt cacatcagcg agcccaggaa tcactttccg ctgcaccatg ccgcccgctc
     7801 gctgctgttt gcgggtggga tcggcgtcac gccgctgctg tgcatggcgc agcgtctggc
     7861 gacgatcggc gccgatttcg cgttgcacta ctgcacccgc tcgcccgagc gcacctcgtt
     7921 tctcgacgag atcgctgcat ccccctttgc cgggcgcgtc cagttccact tcgactcggg
     7981 acccccgggg cagcggctgg acctgcaagc gctgttggcg tcacccgacg ccggaacgcg
     8041 gatctacgta tgcgggccga cgggcttcat cgaccacgtg atcggcagcg cgaaggcgcg
     8101 gggctggaac agcgaacaga tccacctgga atactttggc gcggcgccgc aggacacctc
     8161 gggcgacacg gcgttcgacg tgaagatcgc cagcacgggc aagacctatc aggtcgctgc
     8221 ggggcagagt gtcgtccacg cactgcggga acacgacgtc gagatcctca cctcctgcga
     8281 gcagggcgtg tgcgggacct gcatcacccg cgtgctcgaa ggcgagcccg accaccgcga
     8341 catgtacttc accgaggaag agcgctcgaa gaacgatcag ttcaccccgt gctgctcgcg
     8401 cgcgcggagc aaggcgttgg tgcttgatct gtagattggc ttgttccctc tccccgacgg
     8461 ggagagggag caagccgccc agccccttct gtcctaccat agcaattcca ccaggagaca
     8521 ccatgacccc gctacccgcc cgctacgacc acgtcggcag cttcctgcgg cccaagtccc
     8581 tgctggaagc gcgcgaacgc aaagccaagg gcgagatcac gcccgagcag ttgcgccagg
     8641 tggaggacaa ggccatcggc gaaatcgtga aattccagga ggacgtcggc ctcaagagca
     8701 tcacggacgg cgagttccgc cgcacctatt tccacatcga cttcctcgag cagctcggcg
     8761 gggtgaagac cgacattccg gtgacgatca agaagcccga cggcaccgaa gaactggcgc
     8821 cgccggtgat gcgcgtcatc gacaaggtgc ggcacgccaa gaacatccag ctggcggact
     8881 tccagtactt gaagagccag gtgagcgccg gccacacgcc caaggtctgc atcccctcgc
     8941 ccaccatgct ccacttccga ggcgggcgcg ccggcatcag caaggaagcg tatcccgagc
     9001 tggaacccgt tttctacgac gacgtggcca aggcctacgg cgacgagctg cgctcgctgg
     9061 ctgccgccgg ctgcacctac gtgcagctgg acgacaccaa catggcctac ctgtgcgacg
     9121 agaagatgcg cgaggcggca cgccagcgcg gcgacgaccc gaacgagctg ccgcatcgtt
     9181 acgcgaaatt catcaacaag gtcgtggcgc aaaagcccaa aggcatgctg ctggccatgc
     9241 acctgtgccg cggcaacttc aagagcacgc acgccgccac cggcaactac gagccggtgg
     9301 ccgaggccct gctcaaggaa atggacctgg acgcgtactt catggaatac gacgacgacc
     9361 gctcgggcga cttccggccg ctgcgttacc tgcccaaggg caagacggtc gtgctgggcc
     9421 tggtgaccac caagttcggc gagctcgaaa ataaggaaac gctcaagcgc cgcattgccg
     9481 aagccgcgca gtacgcaccg ctggaacagc tggcgctgtc gccccaatgc ggcttctcca
     9541 gcaccgtgca cggcaacaag atcgccgttg aagcccagcg cagcaagctg cggctggtga
     9601 tcgagacggc gcaagaagtt tggggcgaga attagctccc gcaattcaca gcgaggtcgc
     9661 tcatgctttc gatttcgcga cgccgagcca tggccgcatt gggcacggca agcctgccgc
     9721 tggccgcctg gaggccggcc cggtcgcagc ccggcaggat gcagtcctgg ccgctgggca
     9781 ccccacgccg caccggcatc catgacgtgg cgcctgcgcc cgatgggggc gtctggctca
     9841 cggcccaggc gagcggccac ctgggctggt tcgaccccaa ggccggccgt actgaactga
     9901 tcgctctggg ctccggttcg agcccgcacg gagtgatcca gggacccgac aaggcggcgt
     9961 ggatcaccga cagcggacag aacgcgatcg tgcgggtcgg ctggccggac cgcaaggtgc
    10021 aggctttcgc gttgcccgcc gccgcttcgc gcgccaacct caacacggcg gcgttcgacg
    10081 gcgacggcga cctgtggttc acgggccaag ggggttacgt gggcaaggtc gcgctcaaga
    10141 ccggtcaggt cagcgtgaag gatgccccgc gcgggcgggg accctacggc atctgctcga
    10201 caccgggcgg cgacgtgtgg tggtgctcgc tgggcggttc gttcattgcg cgcatcgacc
    10261 ggcgcaccgg caactcctcc gtcgtcgaac cgcccacccg caaccagggc gcgcgccggg
    10321 tgtggagcga cagcaagggc cgcatctggg tcagcgaatg gaacagcggc aacctgtcca
    10381 tgcacgatcc ccaggccgga acctggcgcc agtggaaagt gccgggcggc aatccccagc
    10441 cctacgccgt gtatgtggat gagcgcgacg tggcgtgggt cagcgatttc ggcggcaacg
    10501 ccgtcttcag cttcgatccg cgcaccgaga agttcgatcg ctacggtttt ccgcgcgaca
    10561 gtgccaacgt gcggcagatt cacggtcgcc cgggcgaggt ctggctgccg gaaagcggca
    10621 ccgaacacat cacggtcatc cgcaagggtt gatgacagcg tcgttcctac cgtcccgcaa
    10681 attgctgccc gagcacactc tggagcagct tgagcacctt ttcgtcgtcg cctaaataca
    10741 gcaccaggag gttggccttc ttgtaaaagt gaggggcgcc tatccagaac ggcttgctgg
    10801 ttcccacctg gctgccgtcg cgcgacactg tcgcggcctg ggcgtcggct gccgcggcgc
    10861 gagggtattc aaaaacctgc acgccctcgc catcaacatt gatcgtcttc gccggaactg
    10921 caaagaaagg ctggtcaacc gagccttccg attgcacagt gccgccggcg gcacgcaggg
    10981 cgtcgaccag attcttgacg ctcagcgtcc cccccgtggg tccggcctct tgcgtacggc
    11041 gggaaatctc cgaattcccg gcgtatgaag cgagggacgc agcacacaag aggagcacgc
    11101 ccacgcgatg aagaaatgag caagacatca gcctcgccca atttcaaaca gggggcgatc
    11161 cggaacgcgg ctaacggtaa acccgatgat cacaactggg gcacgcggac gcggtttctt
    11221 cggtccaata aacgtgcgtc agcgtgtggg ttccgtcccg gttgtaccgc tcgtaaatcg
    11281 agctgcggcc atccgccgac tggcgcatca gggttcccca gctgatgggg tcatcgctca
    11341 ggtcaggcgt ggtcgacgcc gccgaagccg cactggaggc aacggtcgtt gtgctgtcga
    11401 gatgttcgtc gtagatgaag ttcagctgct cgaagtcgtg cgtgttcggg ctgatgctta
    11461 gcgtgctggt ggcattggcg ccggtattgg aaaaataatc catgcaagtg ttcagcgatt
    11521 tgccgtcctc ggactgatgg tcgaggccga acgtatgtgc gatttcctga cacatgacgt
    11581 gctgccgctc attgggattg ttgtatttgg tggcagcaga aagatacgtg tcgttcatct
    11641 tggcggaacc ctgcgtgatg tgatcaccgc tgatgtagat cgttgcaagg cccagccagc
    11701 cgtttttgcc atacttgcca ttgcacacct gggtcgttcc cttcaccatc gagcaacggc
    11761 gattggcacc gcccgtgacg gccaacgtcc agattggagt ggatgattcg ggggggctgt
    11821 tccaatcctc ggacgccgtg tgaaagctga aacgccaatc aaccgatgtc aggttgttac
    11881 cgagctgcaa cgtgaatggc gtagtggtcc gcgcccagtg ataaccgccc catgagtggt
    11941 ttgccagcgc tgagacggat accgtcgcgc ctataagcac ggctgcgtaa attgcgattg
    12001 agcggatagc atgggaatgg aatcgcgagg ctgggttcat gtcttttcct ctccaacttg
    12061 tcagctcacc gacgggcgaa cagctgaaac attaactcac gtctggctca ggaggccagg
    12121 cgtttacaac ctcttacgcg accggcacct a
//
LOCUS       DASWIY010000041         6321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2989395, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000041 DASWIY010000000
VERSION     DASWIY010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2989395"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VGA59_01540"
     CDS             complement(<1..367)
                     /locus_tag="VGA59_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9718387.1"
                     /translation="MCWARCRKPQRHRATYTRKQGTEQLHGFYDVHADCLVGRVRKRK
                     TARDIVACFAQLRACYPMQLRLYIVMDNLSANVRAANDFFPGHNMQAVYLPTNASWLN
                     AIESEFTALHAATLKNSDDP"
     gene            583..951
                     /locus_tag="VGA59_01545"
     CDS             583..951
                     /locus_tag="VGA59_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718388.1"
                     /translation="MLEQIAVQFGHGPVGEGQADIRGPLPGNPNQVLHLSALDSRWPS
                     GRIGRALEAPEPKPIEVGQAAVGRFFCTSDPSPSGQSAQASAHQSNELIAMGYPHRQL
                     PVMHLGFQSLRLATAEAPQL"
     gene            1121..1600
                     /locus_tag="VGA59_01550"
     CDS             1121..1600
                     /locus_tag="VGA59_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9718389.1"
                     /translation="MRGPKPPSLELSEPERDGLQVLVRRRTTPQQLALRARIVLAAAA
                     GSNNSQIARQFGLETDTVRHWRQRWLGLQAVALDDLSVADRLADAPRPGRPAEIGAEQ
                     VCQIVSLACEAPSQSERPISQWTGREIADEIVRREILPHISPRHAARLLKRGRCSRT"
     gene            1597..2331
                     /locus_tag="VGA59_01555"
     CDS             1597..2331
                     /locus_tag="VGA59_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9718390.1"
                     /translation="MIRYWLTPTPDTQFDAKAGDVCTLYQAAPQLAAQGECVVSTDEL
                     TGVQALERTQPGLPLAPGKVERREFEYVRHGTCSFMLNRDVVTGQMLAPSCGPTRTEA
                     DFARHVQKTVASDPHVTRWHFVVDNLNIHCSETLVRWVAEESDLSLDLGVKGQSGILK
                     NLASRAAFLSDPNHRIVFHYTPKHASWLNQIEIWLSILVRKLLRRGNFLSVADLQAKV
                     LAFIDYYNRTMAKPFRWTYQAKPLVA"
     gene            complement(2374..3981)
                     /locus_tag="VGA59_01560"
     CDS             complement(2374..3981)
                     /locus_tag="VGA59_01560"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9718391.1"
                     /translation="MTDYSSAFARLRDYLEAYVRAYRLPGLAVVVTDRDKVLQTFDFG
                     YADLAAQMPVAPETLFQIGSISKSFTAIALLQLHEAGRLDVNAPVTRYLPWFELPSRF
                     APITLHHLLTHTAGIICGQDVSPSAYAEARALRETEAGAEPGTFYLYSNSGYKVLGLV
                     LEEVLQQPYAEIIRERILTPLGMAGAEPVITNDTRQRTAVGYAPAYDDRPASRGAPLA
                     PASWLETDTGDGAIVATSTDMAAYARLLLNRGQGPQGRLLSEAGFGLLTQPAIRTEEA
                     PDEIFYGYGLDISQDEGHPIISHGGSMVGFVSAMLADMQAGLGIVLLSNGQRGVYRAG
                     DAALRLLRGGAVDDPDLGRRVENAGEYLGDFRSPESGKSFTVEAEGEELALRFDGGRA
                     PLERTSQPNEFHVLHPDFTLYLLRFEREGGQVVEAFHGPDWYAGGGYGGPRTFDAPAE
                     WRAIPGHYRAYNPWYSNFRVVLRKGALAVIYSHGEEEPLTLLADGSFRIGADERSPER
                     VRFGELRHGRAEEANLNGGLYYRTFTR"
     gene            complement(4283..4876)
                     /locus_tag="VGA59_01565"
     CDS             complement(4283..4876)
                     /locus_tag="VGA59_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX9718392.1"
                     /translation="MPDNLFALLSYVLISTFTPGPNNISAASLGVVHGYWRTLRYQAG
                     MAAGVWVMMLLAAWVSASLLSSFPAIEPALRWVGAAYVLYLAYSILKATYTFDGGQSK
                     PVGFGRGFLLQLLNPKLVVYGLTLFATFLGPITHSPALLAAAALLAGMAFAATSTWAL
                     FGTAIKVYQRRPRVAALVNTLLALLLVYTAIELSGIL"
     gene            complement(4924..5244)
                     /locus_tag="VGA59_01570"
     CDS             complement(4924..5244)
                     /locus_tag="VGA59_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9718393.1"
                     /translation="MWRARRYVEERYADGLRLQQLAEHVSLSPYYFLRVFRAEVGMPP
                     HAYLDSVRVRHAQRLIEAGEPLARVAADVGYSSQSHFTRSFKRIIGATPGQYARQPGR
                     APAA"
     gene            complement(5519..5935)
                     /locus_tag="VGA59_01575"
     CDS             complement(5519..5935)
                     /locus_tag="VGA59_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718394.1"
                     /translation="MGKRTILIALSVLLYYVVVCAAAVRVRCWWLDIPPRPLVYPGSI
                     LIEERPFLAGRSYHPLVTYYYSSAAAPLEILAFYEQQGRCDIGEETIRAIRRMCSGAA
                     KPFGEYLAYIPMDAKQNEGTPYVIEVRWSACGDDFE"
     gene            6046..>6321
                     /locus_tag="VGA59_01580"
     CDS             6046..>6321
                     /locus_tag="VGA59_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX9718395.1"
                     /translation="MSHILLTNDDGIGHPGLLALKRALDPLGKVTVVAPSRNYSAAGH
                     RKTLHKPLRIDETQMTDGSPAWVCSGAPSDCVALALLGFVKEKVDLVV"
BASE COUNT         1161 a         1981 c         2076 g         1103 t
ORIGIN      
        1 tgggatcgtc tgaattcttg agtgtggcgg catgcaaggc ggtgaactcc gactcgatgg
       61 cgttgagcca ggaagcgttg gtgggcaggt agacggcctg catgttgtgg ccaggaaaga
      121 aatcgttggc cgcacggacg ttggccgaca agttgtccat gacgatatag aggcggagct
      181 gcatcggata gcacgcccgg agctgggcga aacaggccac gatgtctcgc gcggtcttgc
      241 gcttgcgcac tcgcccgacc aagcagtcgg catggacatc gtagaatccg tgcagttgct
      301 ccgtgccctg ctttcgagta taggtcgccc ggtggcgctg gggttttcga caacgcgccc
      361 agcacattcc ggccaccggg cggagttcca aaggacccag ttgatcgtag cagatgacga
      421 ccccccgttt gggacgacag gcatagagcg ccagaattcg ctttttttgg gctcaaactc
      481 cgggtcgtcg gaatgcttcc aggtcttggt tcgctgccag gagatgtctt cccgatggag
      541 caagcggcgt agccactccg ggctgacggc aggaaacaac ttgtgcttga gcaaatagct
      601 gtgcagtttg gtcatggacc agttggtgaa gggcaggccg acatccgtgg gccgcttccg
      661 ggcaatccga accaggtgct gcacctgtcg gcgctggata gccgatggcc gtccggccgg
      721 attggaagag cgctcgaagc gccggaaccc aaaccgattg aagtcggcca ggcagcggta
      781 ggccgtttct tttgcacatc cgatccgtcg cccagcggcc aaagcgctca ggccagcgcg
      841 catcagagca atgagctgat agcgatgggc tacccacacc ggcaacttcc ggtcatgcat
      901 cttggctttc agtctttgcg tctcgccacg gcggaagcgc cgcagctgta accgctttgg
      961 catggctcca gctcctttgg ttggagccag agcatagcaa atctttgtcc tagtttgata
     1021 gttggaacgg cactagtaca gtacggccca aataggccgg tagtatactg tgcgatgtaa
     1081 gctggcccgc atcgggtggg tcggccgtaa aggaaagaag atgcgtggtc ccaagcctcc
     1141 cagccttgaa ctgagtgaac cggaacggga cggattgcag gtgttggtgc ggcggcgcac
     1201 cacaccgcag cagcttgccc tgcgcgcccg catcgtcctg gcggccgccg ccggatcgaa
     1261 caacagccag attgcgcgcc aattcggatt ggagaccgac accgtgcgcc actggcgcca
     1321 gcgttggttg ggcttgcagg cggtggccct ggacgatctg agcgtggccg accggctggc
     1381 ggatgcgccg cggccggggc gcccggccga gatcggagcc gagcaagtct gccagatcgt
     1441 gagcttggcc tgcgaggccc ccagccaatc cgagcgcccg atcagccagt ggaccggacg
     1501 cgagatcgcc gacgaaattg tcaggcgcga gatcctgccg cacatttcgc cgcgccatgc
     1561 cgcccgactg ctaaaaaggg ggcgctgcag ccgcacttga tccgttactg gttgaccccc
     1621 acccccgaca cgcagtttga cgccaaggct ggcgacgtgt gtacgctcta tcaggccgcc
     1681 ccgcagttgg cggcccaggg ggagtgcgtg gtgagcaccg atgaactgac cggcgtgcag
     1741 gcgctcgagc gcacacagcc cggcctgccc ttggcgccgg gcaaagtcga gcggcgcgag
     1801 ttcgaatacg ttcggcatgg gacctgttcg ttcatgctca atcgcgatgt ggtcaccggt
     1861 cagatgctgg ccccgtcctg cggcccaacc cgcacggaag ccgacttcgc gcggcatgtc
     1921 cagaagacgg tcgccagcga cccgcacgtg acccgctggc actttgtggt ggacaatctc
     1981 aacattcact gttcggagac gctggtgcgc tgggtggccg aagagtcgga cctgagcctc
     2041 gacctggggg tcaagggcca gtccggtatt ctgaagaact tggcttcgcg cgccgcgttt
     2101 ctgagcgatc ccaaccatcg gattgtcttt cactacacgc ccaagcacgc ctcctggctc
     2161 aaccagatcg aaatctggct cagcattctg gtgcgcaaac tgctgcgccg cggcaacttt
     2221 ctctccgtcg ccgatctgca agccaaagtg ctggccttca tcgactacta caatcgcacc
     2281 atggccaaac cgttccgatg gacttatcaa gccaagccgc tcgtggccta aaccgcgggc
     2341 ctatttcagc cgcggtgtac tagttatccc gtttcaccgg gtaaacgtgc ggtagtagag
     2401 cccgccgttc aggttggctt cttcggcgcg gccgtgccgc agctcgccaa agcgcacccg
     2461 ttcgggcgat cgctcgtccg cgccgatgcg aaacgaccca tcggccagca gggtcagcgg
     2521 ctcctcctcc ccgtgtgaat aaatcacggc cagcgcgccc ttgcgcagga ccacgcggaa
     2581 gtttgagtac cacgggttgt aagcgcgata atggccgggg atggcccgcc actcggcggg
     2641 cgcgtcgaag gtgcgtgggc cgccatagcc gccgcccgcg taccagtcgg ggccgtgaaa
     2701 ggcctcgacg acctggccgc cctcgcgctc gaagcgcaga agatacaggg tgaagtcggg
     2761 gtgcagtaca tgaaactcat tgggctggga ggtgcgttcg agcggcgcgc gcccgccgtc
     2821 gaagcgcagc gccagctcct cgccctcggc ctcgaccgtg aacgatttgc ccgactcggg
     2881 cgagcgaaaa tcgccgaggt attcgccggc gttctcgacg cggcggccca agtcgggatc
     2941 gtccaccgcc cctccgcgca gcaggcgcaa cgccgcatca cccgcccggt agacgccgcg
     3001 ttggccgttg ctcaacagca cgatgcccag cccggcctgc atatcggcca gcatggccga
     3061 cacgaagccc accatgctgc cgccgtggct gatgatgggg tgaccctcgt cctgcgagat
     3121 atccagcccg tagccgtaga agatttcgtc cggcgcttcc tccgtccgga tcgcgggctg
     3181 ggtcaacagg ccgaagcccg cctccgacaa caggcggccc tgcggcccct ggcctcgatt
     3241 gagcagcagg cgcgcgtagg cggccatgtc ggtggacgtg gcgacaatcg cgccgtcgcc
     3301 ggtgtcggtt tcgagccagg aggccggggc cagcggcgcg ccgcgcgagg cgggacggtc
     3361 gtcgtaggcc ggcgcatagc ccacggccgt gcgctggcgg gtgtcgttcg tgatgaccgg
     3421 ctcggcgccg gccatgccca gcggcgtcag gatgcgctcc cggatgatct cggcgtacgg
     3481 ctgctgcaga acctcctcca gcaccaggcc gagcactttg tagccgctat tggagtagag
     3541 atagaacgtg ccgggctcgg ccccggcctc ggtctcgcgc agggcgcgcg cctcggcgta
     3601 ggccgacggc gacacgtctt gcccgcagat gatcccggcg gtgtgggtga gcaggtggtg
     3661 cagggtgatg ggggcgaagc gcgacggcag ttcgaaccag ggcaggtagc gggtgacggg
     3721 cgcgttcaca tccagccggc ccgcctcgtg aagctggagc agggcgatgg cggtgaacga
     3781 tttgctgatc gagccgatct ggaacaaggt ctcgggcgcg accggcatct gggcggccag
     3841 gtcggcgtag ccaaaatcga aggtctgcaa taccttgtcg cggtcagtca ccaccacggc
     3901 caggccgggc aggcggtagg ctcggacgta ggcttcgagg tagtcgcgca ggcgggcgaa
     3961 ggcggaggag tagtcggtca tcggggccgg tgagttcttc tgtttctcag ccgtcgttgc
     4021 aggcgtcgag gcattagccg gttgatggcc gcacccaatt cgcccggctg aagcctcgac
     4081 tccattgcat tgtaggctga cgacggcgaa tgagcaacag gccatggcgg gacgccgatg
     4141 gcgagtcgct tatcgccgag gaggacgatt gggggtaggc gtggcctagg acaatagcag
     4201 cgggggtacg ttttgggggt gctgtctcag cgtagcacaa cgtttccgtg cgtcggttgc
     4261 aatatctatc gtgtgaaata gttcacaata tgccgctcag ttcgatggcc gtgtagacca
     4321 gcagcagcgc cagcaaggtg ttcaccagcg ccgccacgcg cggccggcgc tggtagacct
     4381 tgatggccgt gccgaacagg gcccaggtgg aggtggcggc gaaggccatc cccgccagaa
     4441 gcgcggcggc ggccaggagc gccggcgagt gcgtgatcgg gccgaggaag gtggcaaaga
     4501 gggtcaggcc gtagacgacc agtttggggt tgagcagttg gagcaggaag ccgcgcccga
     4561 agccgaccgg cttcgactgg ccgccgtcga aggtgtaggt ggccttcagt atcgaatacg
     4621 ccaggtagag aacatacgcc gcgccgaccc agcgcagggc cggctcgatg gccgggaagg
     4681 agctcaacag cgaagcggaa acccaggcgg ccagcagcat catcacccac accccggccg
     4741 ccatgcccgc ttgatagcgc aacgtgcgcc agtagccgtg gacgacgccc agcgaggcgg
     4801 ccgagatatt attcgggccg ggggtgaagg tggagatgag gacgtaagac agcaaggcga
     4861 atagattgtc aggcacgcct acctcgacaa cgcacggtat ggacttgcga ttgcagttgg
     4921 aagttacgcg gcgggcgcgc ggcccggctg gcgcgcatac tggcccggcg tcgcgccgat
     4981 gatgcgcttg aagctgcggg tgaaatggct ctggctgcta tagccgacgt cggcggccac
     5041 ccgcgccagc ggctcgccgg cttcgatcag gcgctgggcg tgccggacgc gcacgctgtc
     5101 gaggtaggcg tgcggcggca tgccgacctc ggcccgaaac acgcgcagga agtaatacgg
     5161 gctgaggctg acgtgctcgg ccaactgttg caggcggaga ccgtcggcat accgttcctc
     5221 gacatagcgc cgcgcgcgcc acaccgcccg gcgctcgtgg cccaggcgct actcgtcgaa
     5281 cctgtcgtcg gcgtgccccc gcccacagcg ccaccgccgg caaaatccgc ggccggccgg
     5341 ggccttgacc caccggttct ggtaccagcc cttcaatgat cggcttcaga aaaccctcga
     5401 tggattcgac cagttcggtg cgttcttgtt cgtctgtcat gcccccattc tgccatgagc
     5461 cgcgatttag ccggaatcaa tgacgcatgc gtcgccccta ccggacgctg attgtgtttt
     5521 actcgaaatc atcaccgcag gctgaccaac gaacttctat gacgtagggc gttccctcgt
     5581 tctgtttggc gtccatcgga atatatgcaa ggtattcgcc gaaaggcttc gccgcgccgc
     5641 tgcacattct acgaattgcc cgtatcgtct cttctccgat gtcgcatctt ccttgttgct
     5701 catagaacgc taagatttcc agcggcgctg ccgcggacga atagtagtag gtcaccagcg
     5761 gatgatacga gcgcccagct aagaacggcc gttcttcgat cagaatggaa cccggatata
     5821 ccaatggtct tggtggaatg tccaaccacc agcagcgaac tcgtaccgcc gccgcacaaa
     5881 ccacgacgta gtagagcagg acggacaggg cgataaggat agttctcttg cccatgtgaa
     5941 gaagtggttg ttagtgaccc ctcacccctc acccctcacc cctatccatc ccttgcaccc
     6001 cccgtcgctc cccattatag tagccaacga caatcagagg caatcatgag ccacattctg
     6061 cttaccaacg acgacggcat cggccatccc ggcctgctgg cgctcaagcg cgcgctcgac
     6121 ccgctgggca aagtcacggt ggtcgcgccc tcgcgcaact attcggcggc cgggcaccgc
     6181 aagacgctgc acaagccgct gcgcattgac gagacacaaa tgaccgacgg ctcaccggcc
     6241 tgggtgtgct cgggcgcgcc ctccgactgc gtggcgctgg cgctgctcgg tttcgttaaa
     6301 gagaaggtgg acctggtggt g
//
LOCUS       DASWIY010000042         3925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3352765, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000042 DASWIY010000000
VERSION     DASWIY010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3352765"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..>3925)
                     /locus_tag="VGA59_01585"
     CDS             complement(180..>3925)
                     /locus_tag="VGA59_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HEX9718396.1"
                     /translation="ILVAGLDFSRGSSNDGRAADWFFRQTEFVIRDGSVRWTDEMRSA
                     PPLALDQVDFVMRNSARGHALRLDATPPSAWGERFSVRGLFRQPLLSTRHGRWQEWEG
                     QAHADFSRVDVSQLRRYANFGFEVADGHGAVRAWADIARGQVTGGAADIVLADVSTTL
                     GAALAPLALRSVSGRIGGKRLDGGFEFETQRLQFETAEGQRWPGGNVFVLWTQAQGKQ
                     PARGEVRADKLDLFALGQIGARLPLGATTHAALQAYAPKGLVETVHAKWEGPLDALRK
                     YEARGRAVGLAVAARPAEAPNGSAARAGTPGFRGADVDFDLTQAGGKGRLRVRGGAVE
                     LPGVFEEPVIPIDALSADVQWQVKGDDISAAVSNLKFSNADAQGEGQVSWRTSEAARA
                     RFPGVLDLQASMSRANGARVHRYLPLGVGKEARDYVRESVVQGKATGAKFRVKGDLRD
                     FPFNDPRQGEFRITADVRDVTYAFVPRSVTKADVAWPALTELSGELVFERNGMQVKRA
                     VGRFAGAPGLKVMAEAQIPSWDKPTVAVTGDVRGPLPQALGIINDSPVSAMIDHALVK
                     ATAAGNADVRLQLALPVATLEKSKVQGSVTLAGNDIQITPDSPRLARSVGAVTFTDRS
                     FALVGTRARALGGEVRLEGGTRAAAAVPLPGSPDAGVILRAQGTATAEGLRQARELGF
                     VSRVARDASGSAAYHLTLAFRRGVPEISVTSNLQGLALNLPPPLAKPAEAVLPMRYEN
                     TLVRDAPAAAAGPQALRDQLSLELGRLVSVTYVRDLSGPEPRVIRGGIAVGVARDESD
                     PMPEQGVMANVNLASVNLDAWEEVLGRVAGGGPAAVSSPRTSPAGPSLGYLPTVMAVR
                     AKELTLEGRTLHNVVVGGSREGLIWRANVDATELNGYLEYRQPAGAGGGRLHGRLARL
                     AIAASAAKEVEALLRQQPDKLPALDVVVDDFELRGKKLGRLEIDAVNRGAGTVAREGG
                     VREWRLNKLNLSMPEATVSATGNWAALNAQAVPPGGPRPGPIPAGEPRRTVMNFKLDI
                     VDSGQLLARLGMKDMVRRGKGRMEGQVSWLGSPLTLDYPSMNGNFYVNVEAGQFLKAD
                     PGLAKLLGVLSLQSLPRRLALDFRDVFSEGFAFDFVRGDVTIEQGIAATNNLQMKGVN
                     AAVLMEGKADIARETQDVKVVVVPEINAGTASLVATVINPAIGLGTFLAQMFLRQPLM
                     RAATQEFQIDGTWADPRITRINRKAPADGRVDAANDPGTGRTN"
BASE COUNT          571 a         1415 c         1345 g          594 t
ORIGIN      
        1 gaacgcccgg ggcgcgcagc tgtccagtgc agcgagccca atgccgggcc tgcactatgc
       61 acgtgccgag gcatgacgct gcggcccggc cccgcaatcc atgccggtgg agggcgctga
      121 cgccgcatgg atcccgggtc aggcccgtga tgacagcagc atgtgggaca ggtttcatct
      181 cagttggtcc ttcccgtgcc cggatcgttc gcggcgtcga cccggccatc ggcgggcgcc
      241 ttccggttga tccgcgtgat gcgggggtcg gcccaggtgc cgtcgatctg gaattcctgg
      301 gtggccgcgc gcatcagggg ctggcgcagg aacatctgcg ccaggaaggt ccccagcccg
      361 atcgcggggt tgatcacggt ggccaccagc gaagccgtgc cggcgttgat ctccggaacg
      421 accaccacct tgacgtcctg cgtttcgcgc gcgatatcgg ccttgccttc catcagcacg
      481 gccgcgttca cgcccttcat ctggaggttg ttggtggccg ctatgccctg ctcgatggtg
      541 acatcgccgc gcacgaaatc gaaagcgaag ccctcgctga agacatcgcg gaaatccagc
      601 gccagccgcc gcggcagcga ttgcaggctg agcacgccca gcagcttggc cagcccgggg
      661 tcggccttga gaaactggcc ggcctcgacg ttgacataga aattgccgtt catcgacggg
      721 tagtccagcg tcagcggcga acccagccac gacacttgcc cttccatgcg tcccttgccg
      781 cgccgcacca tgtctttcat gcccaagcgc gccagtagct gccccgagtc gacgatgtcc
      841 agcttgaagt tcatgaccgt gcggcgtggc tcgccggcgg gaatggggcc tggccgggga
      901 ccccccggcg gcaccgcctg tgcattcagt gcggcccaat tcccggtggc gctgaccgtg
      961 gcctccggca tgctgaggtt gagcttgttc agccgccact cgcgcacacc gccctcgcgc
     1021 gccacggtgc cagcgccgcg gttgaccgcg tcgatctcga ggcgccccag cttcttgccg
     1081 cgcagctcga agtcatccac cacgacgtcc agcgcgggca gcttgtcggg ctgctggcgc
     1141 agcagcgcct cgacctcttt ggccgccgag gccgcgatgg ccaggcgcgc cagccgcccg
     1201 tgcagccgcc cgccgcccgc gccggcgggc tgccggtatt cgagatagcc gttgagttca
     1261 gtggcgtcga cgttggcgcg ccagatcagg ccttcccggg agccgccgac caccacgttg
     1321 tgcagcgttc gcccctccag ggtgagttcc ttcgcgcgca ccgccatcac tgtgggcagg
     1381 tagcccaatg aggggccggc gggcgaggtg cgcggggagg agacggctgc cggcccgccg
     1441 ccggcgaccc ggcccagcac ctcttcccat gcatccaggt tgacgctggc caggttgacg
     1501 ttggccatca cgccttgttc cggcatcgga tcggactcgt cccgggcgac gccgaccgcg
     1561 atgccgccac ggatcacgcg cggctcgggg ccggacaggt cgcgcacata ggtgacggaa
     1621 accaagcgcc ccagttcgag cgacagctgg tcgcgcaacg cctgcggccc ggccgcggcg
     1681 gccggtgcgt cccggaccaa cgtgttctcg tagcgcatcg gcagcaccgc ttcggccggt
     1741 ttggccagag gcggcggcag gttcagcgcc aggccctgca ggttgctggt caccgagatc
     1801 tcgggcacgc cgcgccggaa ggccagcgtg aggtggtaag cggcactgcc gctagcgtcc
     1861 ctggccacgc gcgagacgaa gcccagttcc ctggcctggc gcaggccttc ggcggtggcc
     1921 gtgccctgcg cgcgcaggat cacgccggcg tccggactgc ccggcagcgg caccgcggcg
     1981 gcggcccggg tgccgccttc cagccgcacc tcgccgccga gcgcgcgcgc gcgcgtcccc
     2041 accagggcaa agctgcggtc ggtgaaagta acggcgccca ccgatcgcgc gagccggggg
     2101 ctgtcgggcg tgatctggat atcgttgccg gccagggtga cgctaccctg gaccttggac
     2161 ttttcgaggg tggccacggg cagcgccagc tgcaggcgca cgtcggcgtt gccggcagcg
     2221 gtggccttaa ccagggcgtg gtcgatcatg gcggacaccg gtgaatcatt gatgatgccc
     2281 agcgcttgcg gcagcggccc acgcacatcc cccgtcacgg ccaccgtggg tttgtcccag
     2341 ctggggatct gggcctcggc catgactttc aggccgggcg caccggcgaa gcggccgacc
     2401 gcgcgcttga cctgcatgcc attgcgctcg aacaccagtt cacccgacag ttcagtcagg
     2461 gccggccagg ccacatccgc cttggtgacc gagcgcggca cgaaggcata ggtcacgtcg
     2521 cggacatcgg cggtgatgcg gaactcgccc tgccgcgggt cgttgaaggg gaagtcgcgc
     2581 aggtcgccct tgacgcggaa cttggcgccg gtcgccttcc cctggaccac cgattcgcgc
     2641 acgtagtcgc gcgcctcctt gccaacgccc agcggcaggt agcgatgcac cctggcgcca
     2701 ttggcccggc tcatacttgc ctgcaggtcg aggacgccgg gaaagcgggc gcgcgccgcc
     2761 tcgctggtgc gccagctgac ttggccctcg ccctgggcat cggcattgct gaatttcagg
     2821 ttggatacgg ccgccgagat gtcgtcgccc ttgacctgcc actgcacgtc ggccgaaagc
     2881 gcatcgatcg ggatcaccgg ttcctcgaac acgcccggca gttccacggc gccgccgcgc
     2941 acccgcagcc tgcccttgcc accggcctgc gtcaggtcga aatcgacgtc ggcaccccgg
     3001 aaacccggcg tgccggcgcg cgcagccgac ccgttcggcg cctcggcggg acgggccgcg
     3061 actgccagcc ccacggcgcg gccgcgagct tcgtacttgc gcaacgcatc gagcgggcct
     3121 tcccatttcg cgtggacggt ctcgaccagg cccttgggcg cataggcctg cagcgcggca
     3181 tgggtggtcg cacccagcgg cagccgcgcg ccgatctgcc cgagggcgaa caggtccagc
     3241 ttgtcggcgc ggacctcgcc tcgggccggc tgcttgccct gggcctgcgt ccacagcacg
     3301 aagacatttc cacccggcca gcgctggcct tcggctgttt cgaactgcag ccgctgggtc
     3361 tcgaactcga agccgccgtc caggcgcttg ccgccgatac gcccggatac cgagcgcagg
     3421 gccaggggcg ccagcgcggc gcccagcgtc gtgctgacat cggccagcac gatgtcggcc
     3481 gccccgccgg tcacctggcc tcgcgcgata tcggcccagg cccgcacggc gccatgaccg
     3541 tcggcgactt cgaagccgaa attggcgtag cggcgcagct gcgacacatc gacgcgggag
     3601 aagtcggcat gggcctgccc ctcccattcc tgccaccggc catgccgggt ggacaacagc
     3661 ggctggcgaa agaggccgcg cacgctgaat cgctcgcccc aggcggacgg cggcgtcgcg
     3721 tccagccgca aggcatggcc gcgcgcactg ttgcgcatga cgaagtcgac ctggtccagc
     3781 gcgagcggcg gggcgctgcg catttcatcg gtccagcgca ccgacccgtc gcggatcacg
     3841 aattcggttt gccggaagaa ccagtcggcg gcgcgcccgt cgttgctgct gccgcgggag
     3901 aagtccaggc cggccacgag gatat
//
LOCUS       DASWIY010000043         8202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1141932, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000043 DASWIY010000000
VERSION     DASWIY010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141932"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VGA59_01590"
     CDS             complement(<1..793)
                     /locus_tag="VGA59_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9718397.1"
                     /translation="MKSLIAEQVDSAPVPELIAATEQAMPHQANALARARAFAEPFIA
                     GETLDTGQNTLAHADAVAAILKAMGGSEAMQAAAYLVYACPHLTKPQEVIAKAFGENY
                     AALAVETTKLVRVQQQTRAAEDSARLVDDPKVQTENVRKMLLAFSRDLRVVLLRLASR
                     LQTLRWYAASKSVAPPSVAREALSVFAPLANRLGIWQLKWEMEDLAFRFLEAETYKKV
                     ARLLDEKRAEREEYVEHLRARLESELKAQGVQASVHGRPKHIYSIV"
     gene            complement(765..1700)
                     /locus_tag="VGA59_01595"
     CDS             complement(765..1700)
                     /locus_tag="VGA59_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9718398.1"
                     /translation="MPEPTLNYVTCPDPSGGHGMAYWQWGETPAGHVIVCVHGLSRQG
                     RDFDVLAQALLERAGRPLRIVCPDVAGRGRSDWLKDPQGYQVPTYVADMLALLASLHQ
                     QAAVTTLDWLGTSMGGLIGMAVAGTPQLPLPVPVRRLVLNDVGPAIQWQALQRIGTYL
                     GNTGRFETMEQAAAAMWAISTSFGPHTPQQWLALSQPMVRPLPEGGFTLHYDPAIAVP
                     FRSVTEESAAQGQAALWQLYDNVQAEVLVTRGANSDLLAHDTAVAMTQRGPKARLVEF
                     ESVGHAPTFVANNQIEAVASFLLRQVDEKPDSGAS"
     gene            1777..2547
                     /locus_tag="VGA59_01600"
     CDS             1777..2547
                     /locus_tag="VGA59_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX9718399.1"
                     /translation="MLKGKTALVTGSTSGIGLGIAKALARQGANIVLNGFGDVEGPKA
                     QVQSLGAKVAYHGADMSKPAEIEEMMKFAAAQFGRVDVLVNNAGIQHVAAVEDFPPGK
                     WDAIIAINLSSAFHATRLALPAMKAANWGRIINLASVHGLVASAQKAAYVAAKHGLVG
                     LTKVVALETATTGVTCNAICPGWVLTPLVQQQVDAKAAANGWSNEEATRQLLGEKEPS
                     LQFTTPEELGELAVFFCSPAANNVRGVAWNMDGGWAAQ"
     gene            complement(2550..3266)
                     /locus_tag="VGA59_01605"
     CDS             complement(2550..3266)
                     /locus_tag="VGA59_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797028.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX9718400.1"
                     /translation="MKNTSKAGRAIAAWVLATAAGVSAQGLPYPPPQNVLQLSATGTV
                     EVQQDLLSLSLTTTREGADPNAVQSQLKLALDAALSEARKSAQPGQLDVRTGNFALYP
                     RHTEGGRISTWQGTAELVLEGRDFARITQTAARIQTMTLGGVSFGLSREQRAKVEGDA
                     QAIAIDRFKAKAAELAKGFGFSGYSLREVAVNTNDQGFPRPVRALAMEARAAQADMPV
                     PVEAGKSTVMVTVSGTVQLK"
     gene            complement(3329..3550)
                     /locus_tag="VGA59_01610"
     CDS             complement(3329..3550)
                     /locus_tag="VGA59_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718401.1"
                     /translation="MKMYLPLIASLAAAVCHAQTSPSGHPTSGVRQVSVQQQPAPAPA
                     VTSRQLSVQERAELRRQLSDFSRRAGKGS"
     gene            3715..4446
                     /gene="ompR"
                     /locus_tag="VGA59_01615"
     CDS             3715..4446
                     /gene="ompR"
                     /locus_tag="VGA59_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEX9718402.1"
                     /translation="MTQAAARTDKILVVDDDARIRDLLRRYLTQEGFEVILAEDGKAL
                     NRLMLRETADLIVLDLMMPGEDGLSICRRLRAANDRTPIIMLTAKGEDVDRIVGLEVG
                     ADDYLGKPFNPRELLARVHAVLRRRPAQEAPGAPSSDNEVVTFGPFAFDLGARTLRKN
                     GEELPLTTGEFAMLKALVRHPRQPLSREKLAQLARGREFEPFDRSLDVQVSRLRKLVE
                     SDPAAPRYIQTVWGVGYVFVPDGAG"
     gene            4589..5965
                     /locus_tag="VGA59_01620"
     CDS             4589..5965
                     /locus_tag="VGA59_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9718403.1"
                     /translation="MRPRLGLSLFWRTFFLLSLLLIGSIVAWLQTFSALEFEPRAVQT
                     AQQIASLVNLSRAALVHSDSIARVSLIKTLAEQEGVRILPREPTDRYATFGPGDLDRG
                     ITDELTSRLGPGTVIAASVNGEQGLWIGFRIDTDQYWMLLDRARFARVGGKTWLIWLI
                     TAAGLSLAGAALIARLINRPLKQLSFAASRVRDGDFDASRLDEKAVTSEIREVNIGFN
                     RMAQQLAKIEQDRAVMLAGISHDLRTPLARLRLETEMSVADKDARDHMAADIDQLDAI
                     IDKFLDYARPDHAELKPVLLNDVIEACLYAVQDQGDMRINLDVSKGLLVLADEVELAR
                     VISNLLENARRYGKTPETGIAIVDITAKSRNEWVLLKVRDHGMGVAPEALPNLIKPFF
                     RGDAARTAATGAGLGLAIVDKTVQRMGGIFALANTTSGGLAAHIKLQKPKAPTAPAEI
                     EAKAEPAY"
     gene            complement(6090..6575)
                     /gene="ispF"
                     /locus_tag="VGA59_01625"
     CDS             complement(6090..6575)
                     /gene="ispF"
                     /locus_tag="VGA59_01625"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX9718404.1"
                     /translation="MTFRIGEGWDLHALAPGRKLIIGGVEIPFDRGLLGHSDADVLLH
                     AITDALLGAAGLGDIGRHFPDTDERFRDADSVLLLTEAARRVHEQGLRIGNVDSTVIA
                     QAPRLAPYVDAMRDRIAAALDLGPGLVNVKAKTAEKLGPVGQGLAIEARAVVLLTVRG
                     S"
     gene            complement(6628..7329)
                     /gene="ispD"
                     /locus_tag="VGA59_01630"
     CDS             complement(6628..7329)
                     /gene="ispD"
                     /locus_tag="VGA59_01630"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX9718405.1"
                     /translation="MKTSESKSHSRFFALVPCAGTGSRAGTNGPKQYERIAGQPMVWH
                     TLAALAAVRRISRTVVVVAPGDGFFERNPTSALVVACGGPTRAASVANGLYELTRVGA
                     SQQDWVLVHDAARCLITPKLIDSLIDACAHDEVGGLLARKLPDTLKREEGGRVAATLD
                     RDDKWLAQTPQMFRIGLLLHALERANGHLTDEAAAIEALGHRPLLVPGSIQNFKVTFP
                     DDFAMAEAVLKGRAR"
     gene            7396..>8202
                     /locus_tag="VGA59_01635"
     CDS             7396..>8202
                     /locus_tag="VGA59_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX9718406.1"
                     /translation="MDLPKLRLGKRYTLPRPPGSADALLLARVAGRAKAARQLTAIVT
                     ADATDAQRLIEEIAFFAPDLRCALFPDWETLPYDTFSPHQDLISERLATLWRISQGEA
                     DVVVVPATTVLYRVAPPSFLAGYTFQFQVKQKLEEARLKAQLTLAGYSHVTQVVSPGE
                     YAVRGGLIDLFPMGSTVPYRVDLFDDEIDSIRTFDPDSQRSLYPVPEVRLLPGREFPM
                     DDEARAKFRSRWRELLEGDPTKSRIYKDIGNGVATAGIEYYLPLFFDDTAT"
BASE COUNT         1369 a         2764 c         2681 g         1388 t
ORIGIN      
        1 tgacgatgct gtagatgtgc ttgggccggc catgcacgct ggcctgcaca ccctgggcct
       61 tgagttccga ttccaggcgc gcccgcaaat gctctacgta ctcctcccgc tcggcgcgct
      121 tctcgtccag cagccgggca accttcttgt aggtttccgc ttcgaggaag cggaacgcca
      181 ggtcctccat ctcccatttg agctgccaga tgcccagccg gttggccagc ggggcgaaca
      241 cgctgagcgc ttcgcgcgcc acgctgggcg gcgcgacgct tttcgaggcg gcgtaccagc
      301 gcagcgtctg cagccgcgac gccagccgca gcagcaccac ccgcaggtcg cgggagaacg
      361 ccagcagcat cttgcgcacg ttctcggtct gcaccttcgg gtcgtccacc agccgggccg
      421 agtcttccgc cgcgcgcgtc tgctgctgga cgcgaacgag tttggtggtc tcgaccgcca
      481 gcgccgcgta gttctcgccg aaggccttgg cgatcacctc ttgcggcttg gtcaggtgcg
      541 ggcaggcata gaccaggtag gctgccgcct gcatggcttc ggacccgccc atggccttca
      601 ggatggcggc cacggcgtcg gcatgcgcca gcgtgttctg cccggtgtcg agtgtttcgc
      661 ccgcgatgaa gggctccgcg aaggcacggg cgcgtgccag tgcgttggcc tggtgcggca
      721 tcgcctgctc ggtggcggcg atcagttcgg gaaccggggc ggagtcaact tgctccgcta
      781 tcaggctttt catcgacttg tctcagaagg aacgaggcca cggcctcgat ctggttgttc
      841 gcgacgaagg tgggggcatg tcccacgctt tcaaattcga caaggcgcgc tttcggtccg
      901 cgctgcgtca tggcgaccgc ggtgtcgtgc gccagcaggt ccgagttcgc gccccgcgtg
      961 accaggactt cagcctggac gttgtcgtac agctgccaga gcgccgcctg gccctgggcc
     1021 gccgactcct cggtcacgct gcgaaagggc accgcaatgg ccgggtcgta gtgcagcgtg
     1081 aagccgcctt cgggcaacgg cctgaccatg ggctgcgaga gggccagcca ctgctggggc
     1141 gtgtgcgggc caaagctggt cgaaatggcc cacatcgcgg cggcggcctg ctccatcgtt
     1201 tcgaaacggc cggtattgcc caggtacgtg cctatgcgct gcaaggcttg ccactggatc
     1261 gccggcccca catcgttgag cacgaggcgc cgcacgggca ccggcagcgg caactggggc
     1321 gtaccggcga ccgccatccc gatcaggccg cccatgctgg tgccgagcca gtcgagcgtc
     1381 gtgacggcgg cttgttgatg gaggctcgcc agcaacgcca gcatgtcggc cacgtaggtg
     1441 ggtacctggt agccctgtgg gtccttcagc cagtcgctgc ggcctcgccc ggccacgtcg
     1501 ggacacacga tgcgaagcgg gcggccggcc cgttccagca gcgcctgcgc cagcacgtcg
     1561 aagtcccggc cctggcgcga aagtccgtgc acgcagacga tcacatggcc cgccggcgtt
     1621 tcgccccact gccaataggc catgccgtgc ccgcccgaag gatcggggca cgttacgtag
     1681 ttcagcgtag gttcgggcat ggccgggtaa aaccccgata ctttgcgttt ttgtcgattg
     1741 tctcgtcacc atcctaattc ttttcggaga ctcatcatgc tcaaaggcaa aaccgctctt
     1801 gtcacgggtt ccaccagcgg catcggcctg ggtattgcca aggcgctggc ccgccagggc
     1861 gccaacatcg tgctcaatgg cttcggcgac gtcgaagggc cgaaggccca ggtccagtcc
     1921 ctgggcgcga aggtcgccta ccacggcgcc gacatgagca agcccgccga gatcgaggag
     1981 atgatgaagt tcgcggcggc gcagttcggc cgcgtcgacg tgctggtgaa caatgccggc
     2041 atccagcatg tcgcggcggt agaggacttt ccgcccggga aatgggatgc catcatcgcc
     2101 atcaatctct cgagcgcgtt ccatgccacg cgcctggcgt tgccggcgat gaaggcggcc
     2161 aactgggggc gcatcatcaa cctggcctcc gtccacggcc tggtggcctc cgcgcaaaag
     2221 gcggcctatg tcgccgccaa gcacggcctg gtggggttga ccaaggtcgt ggccctggaa
     2281 accgccacca cgggcgtcac ttgcaacgcg atctgccccg gctgggtgct gacgccgctg
     2341 gtgcagcagc aggtggatgc caaggcggcg gccaatggct ggtccaacga agaggccacc
     2401 cgccagctgc tcggcgagaa ggagccttcg ctccagttca ccaccccgga agagctgggc
     2461 gaactcgcgg tgttcttctg ttcgcccgcg gcgaacaatg tgcgaggggt cgcgtggaac
     2521 atggacggcg gttgggcggc ccagtagcgt tatttcaact gcaccgtccc agaaaccgtg
     2581 accatcaccg tgctcttccc ggcttccacc ggcacgggca tatcggcctg cgctgcccgc
     2641 gcctccatgg ccagcgcccg caccggccgc gggaaaccct ggtcattggt gttgaccgcc
     2701 acctcgcgca agctgtagcc cgagaagccg aagcccttgg cgagctcggc cgccttggcc
     2761 ttgaagcggt cgatcgcgat cgcctgcgca tcgccttcca ccttcgcccg ttgctcgcgg
     2821 ctgaggccga agctcacgcc gccgagggtc atcgtctgga tgcgcgccgc cgtctgggtg
     2881 atcctggcaa agtcccggcc ctcgagcacc agctcggccg tgccctgcca ggtgctgatc
     2941 ctgccgccct cggtgtggcg cgggtacagc gcgaagttgc cggtgcgcac gtcgagctga
     3001 cccggctgcg cggacttgcg cgcctcggac agggccgcat cgagcgccag cttgagctgg
     3061 ctttgcaccg cattggggtc cgcgccttcg cgtgtcgtgg tcaggctcag gctcagcagg
     3121 tcctgttgca cttccacagt ccctgtggcg gacagttgca ggacgttctg cggcggtggg
     3181 taaggcaggc cctgcgccga cacgcccgcc gcggtggcga gcacccaggc agcaatcgcg
     3241 cggccggctt tcgatgtatt tttcatggat gcagatctca cgaagacggg ccgccagcgt
     3301 acagcggaca aagaacgtca cgaaagtttc acgagccctt gccggcccgg cggctgaaat
     3361 cggacagctg gcggcgcagt tccgcccgct cctggaccga cagctggcgc gatgtgaccg
     3421 cgggtgccgg cgccggctgc tgctgcaccg acacttgccg cacgccggaa gtcgggtgac
     3481 ccgagggcga cgtctgcgcg tggcataccg ccgcggccag gctggcaatc agggggaggt
     3541 acatcttcat ggtcagcatt aaacgagtgc cgaatccatg ggttcacaca cgccaagtca
     3601 acgcttgcgc cgcagggcaa catttgtaac actgtgtcaa ggccagggga gaaacccgca
     3661 cgcccggctt caagcccctc aaaatcggct ctgttacata tccattggag caccatgacc
     3721 caagctgcag cccgtaccga caagatcctc gttgtcgacg atgacgctcg catccgcgac
     3781 ctgctgcgcc gctacctgac tcaggaggga ttcgaagtga tcctggccga ggacggcaag
     3841 gcgctgaacc gtctcatgct gagggaaacc gccgacctga tcgtgctgga cctgatgatg
     3901 ccgggcgaag acggactctc catctgccgg cgcctgcgcg cggccaatga ccgcacgccc
     3961 atcatcatgc tgacggccaa aggcgaggat gtcgaccgca ttgtcgggct ggaggtcggc
     4021 gcggacgact acctcggcaa gcccttcaat ccgcgcgaac tgctggcccg cgtccatgcc
     4081 gtgctgcggc gccgtccggc ccaggaggcg cccggcgccc cgtcgagcga caacgaagtc
     4141 gtcactttcg ggccttttgc cttcgacctg ggcgctcgga ccctgcgtaa aaacggtgag
     4201 gaactgccgc tgacaacggg cgaattcgcc atgctcaaag ccctggtgcg gcacccgcgc
     4261 cagccgctgt cgcgcgaaaa gctggcccag ctggcgcgcg ggcgcgagtt cgagcccttc
     4321 gaccgcagcc tggacgtgca ggtgtcgcgt ctgcgcaagc tggtggagtc ggacccggcc
     4381 gcaccgcgtt acatccagac agtgtggggt gtaggttacg tattcgttcc cgacggcgcc
     4441 ggttgattgc gctccccgca gctcgcaaac aataatcagg aggatggcat acacatgagc
     4501 aggggaccga tacttgattc gcgcagtcac cagaagcatc gacgtcaccg gcccggcgcc
     4561 gcttgaaaca gcgccggcgc cgctggacat gcggccgcgg cttggccttt cgctgttctg
     4621 gcgcaccttc ttcctgctct cgttgctgct catcggcagc atcgtggcct ggctgcagac
     4681 gttcagcgcg ctggagttcg agccgcgtgc ggtgcagacg gcccagcaga tcgcttcgct
     4741 ggtgaacctc agccgtgccg ccctggtgca ctcggattcg atcgcccgcg tttcgctcat
     4801 caagaccctg gccgagcagg aaggtgttcg catcctgccg cgcgaaccga ccgaccgcta
     4861 cgccacgttc ggccccggcg atctggaccg cggcataacg gacgagctga ccagccggct
     4921 gggacccggc accgtgatcg ccgcgagcgt caacggcgag caaggcctgt ggatcggctt
     4981 caggatcgat accgaccagt actggatgct gctggaccgt gcgcgttttg cccgagtcgg
     5041 cggcaagacc tggctcatct ggctgatcac cgccgccggg ctttcgctgg cgggcgcggc
     5101 attgatcgcg cggctgatca accgcccgct caagcagctt tccttcgcgg ccagccgcgt
     5161 gcgcgacggc gatttcgacg ccagccggct ggatgaaaaa gcggtgacca gcgaaattcg
     5221 cgaggtcaat atcggattca accggatggc gcagcagctc gccaagatcg aacaggaccg
     5281 cgccgtgatg ctggccggga tttcgcacga tctgcgcacg ccattggcgc gcctgcggct
     5341 ggaaaccgaa atgagcgtgg ccgacaagga cgcgcgcgat cacatggcgg ccgacatcga
     5401 ccagctcgac gccatcatcg acaagttcct cgactacgcc cggccggacc atgccgagct
     5461 caagccggtg ctgctcaacg acgtgatcga ggcctgcctc tacgccgtgc aggaccaggg
     5521 cgacatgcgc atcaacctcg acgtatccaa gggcctgctg gtgctggcgg acgaagtgga
     5581 gctggcgcgc gtcatttcca acctgctcga aaacgcccgg cgctacggca agacgcccga
     5641 aacgggaata gcgatcgtcg acatcacggc caagtcgcgc aacgaatggg tgctgctgaa
     5701 ggtccgcgac cacggcatgg gcgtcgcgcc cgaggccctg cccaacctga tcaaaccgtt
     5761 cttccggggc gacgccgcgc gcaccgccgc gacgggcgcc gggctgggcc tggccatcgt
     5821 cgacaagacc gtgcagcgca tgggaggcat cttcgcgctg gccaacacca cttcgggcgg
     5881 gctggccgcg catatcaagc tgcaaaagcc gaaagcaccg accgcgcccg ccgaaatcga
     5941 ggccaaggcg gagccggcgt actgatttgt catgccggac tcgggacctg atccgggggc
     6001 atccatgcct taagcgatca tggattgcgg atcggggctg ccccggacca gatccggggt
     6061 ccgcaatgac agcctgccca gagccgcgtt cacgacccgc ggaccgtcag cagcaccacc
     6121 gcccgcgctt cgatcgccag cccctgcccg accgggccca gcttctcggc ggtcttggcc
     6181 ttgacgttga ccaggccggg ccccaggtcc agcgccgccg cgatgcgatc gcgcatggca
     6241 tcgacgtagg gcgccagcct cggggcctgc gcgatcacgg tgctgtcgac gttgccgatg
     6301 cgcaggccct gttcgtgcac gcgccgcgcc gcctcggtga gcagcagcac ggagtcggca
     6361 tcgcgaaaac gctcgtcggt gtccggaaaa tgccggccga tatcgcccag gccggcagcg
     6421 cccagcagcg catcggtgat ggcgtgcagc aatacatcgg catcggaatg gccgagcaag
     6481 ccgcggtcga acggaatttc cacgccgccg atgatcagtt tgcggccggg cgccaaggca
     6541 tgcaggtccc agccttcgcc gatgcgaaat gtcatcgtga tgcccgccgc gccttgtggc
     6601 gcatcagatg aagagcgtgg tcctcgctca cctcgcccgg cccttcagca cggcctcggc
     6661 catcgcgaag tcatcgggga aggtgacctt gaaattctgg atgctcccgg gcaccagcag
     6721 cgggcggtgc ccgagcgcct cgatcgcggc cgcttcgtcg gtgagatggc cattcgctcg
     6781 ttccagagcg tgcaacaaca ggccgatgcg gaacatctgc ggcgtctgcg caagccactt
     6841 gtcgtcgcga tcgagggtcg cggcgacgcg gccgccctct tcgcgcttga gggtgtcggg
     6901 cagtttgcgc gcgagcaggc ctcccacttc atcgtgcgcg caggcgtcga tgagcgagtc
     6961 gatgagtttg ggcgtgatca ggcatcgcgc cgcgtcgtgc accagcaccc agtcctgctg
     7021 actggctccc acgcgcgtga gctcgtacag gccgttggcg acgctggccg cgcgggtcgg
     7081 gcccccgcac gccaccacca gagccgaagt gggattgcgc tcgaagaagc cgtcgcctgg
     7141 ggccaccacc accactgtcc tggagatgcg ccgcaccgcc gcgagggccg ccagggtgtg
     7201 ccacaccatg ggctgtcccg cgatccgctc gtattgcttg gggccattgg tgccggcgcg
     7261 gctgccggtt ccggcacaag ggacaagcgc gaagaaacgg gaatgtgatt tgctttcgct
     7321 cgttttcata ctgtccattc tagaatcgag tctcacaccc caccctgttc aggcgcgggg
     7381 tgttttgtat tgtttatgga tttacccaaa cttagattag gcaaacggta cacccttccg
     7441 cgcccgcccg gctccgccga tgccctgctg ctggcccgcg tggccgggcg cgcgaaggct
     7501 gcaaggcagc tgacggccat cgtcaccgcc gacgctacgg atgcgcaacg gttgatcgag
     7561 gagatcgcgt ttttcgcgcc ggatctgcgc tgcgcgcttt tcccggactg ggaaacgctg
     7621 ccttacgaca ccttttcgcc tcaccaggac ctgatcagcg aacgtttggc cacgctctgg
     7681 cgcatttcgc agggcgaagc cgatgtggtg gtggtgcccg caaccacggt gctgtaccgc
     7741 gtggcgccgc ccagcttcct ggcgggctat accttccaat tccaggtcaa gcagaagctc
     7801 gaagaagcca ggctcaaggc ccagctgacg ctggcaggct acagccatgt gacccaggtg
     7861 gtcagcccgg gcgagtacgc ggtgcgtggc ggattgatcg acctcttccc catgggctcg
     7921 acggtgccgt atcgcgtgga cctgttcgat gacgagatcg actcgatccg caccttcgac
     7981 cctgacagcc agcgcagcct gtacccggtg cccgaggtca ggctgctgcc gggccgcgag
     8041 ttcccgatgg atgacgaggc gcgtgcgaaa ttccgcagcc gatggcgcga gctgctggag
     8101 ggcgacccga ccaagagccg catctacaag gacatcggca acggcgtggc caccgccggc
     8161 atcgagtact accttccgct gttcttcgat gacacggcga cc
//
LOCUS       DASWIY010000044        13747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2249949, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000044 DASWIY010000000
VERSION     DASWIY010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2249949"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..182)
                     /locus_tag="VGA59_01640"
                     /pseudo
     CDS             complement(75..182)
                     /locus_tag="VGA59_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465411.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            424..987
                     /locus_tag="VGA59_01645"
     CDS             424..987
                     /locus_tag="VGA59_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718407.1"
                     /translation="MIVQTALSLAEEAGSWDAVHVHAIARAAGISLEELRRHFGDKDA
                     IAEGFFDIADEALLSVCKEPGWGEQETRERLYRAVMSWLDALAPHRSLAVGMLGYKFH
                     PEHLHLQARGVARISRTVQWIREVSMLPSIGWRRDLEEAVLTTIYLTTFSYWLTDTSP
                     GAERTRGFLRRLLAGAEKAALWLGSSA"
     gene            1199..1639
                     /locus_tag="VGA59_01650"
     CDS             1199..1639
                     /locus_tag="VGA59_01650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX9718408.1"
                     /translation="MKKSISGLLPVAALALVAGAAIAATPAAAMPGATETLPGGIVVK
                     HAKVGTGASPVAADTVQVHYRGRLADGTEFDSSYKRGKPASFPLGRVIPCWTQGLQKM
                     KVGGSATLTCPPDTAYGSRGAGGAVPPNATLTFEVELLAIDKKS"
     gene            complement(1707..1955)
                     /locus_tag="VGA59_01655"
     CDS             complement(1707..1955)
                     /locus_tag="VGA59_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HEX9718409.1"
                     /translation="MNEPTQLPPLPDRLSVDPRSPHHVAAVFEHGVGIRLNGNERKDV
                     EEYCLSEGWVKVPAGKTLDRKGNPLLIKLKGKVEAFYR"
     gene            complement(1988..2740)
                     /gene="fnr"
                     /locus_tag="VGA59_01660"
     CDS             complement(1988..2740)
                     /gene="fnr"
                     /locus_tag="VGA59_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEX9718410.1"
                     /translation="MPATQVAVPASAAPVVQLKTQCSTCHLRELCLPCGLNGVDLTRL
                     DDMTFTRRKIKAGQTLYREGDRFQYIYAVRSGTLKSSLLLSDGREQVSGFHIAGELVG
                     LDGAAQGHHASAAMALEDTELCAIPFAHLCDLAAGTTGMQHVITRLMSREILREHSLM
                     MLLGSMSAEERLAAFLLNLSQRYAARGYSEREFHLRMSRAEIGSYLGLKLETVSRTFT
                     SFQQQRLLEVDKRHIRITDLEGLTRRLDVRVH"
     gene            complement(2815..3180)
                     /locus_tag="VGA59_01665"
     CDS             complement(2815..3180)
                     /locus_tag="VGA59_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9718411.1"
                     /translation="MKVFIAEDSAIVRDRVAAMLSEREISVVGHGETPQACIDGILAA
                     HPDVVVLDVRLDGGTGLQVLRAVHQAEPGIGFVVFSNNAGAAYRKRYLGEGAQRFLDK
                     TTEMDQLVAAVENISTQSH"
     gene            complement(3830..4243)
                     /locus_tag="VGA59_01670"
     CDS             complement(3830..4243)
                     /locus_tag="VGA59_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HEX9718412.1"
                     /translation="MDTIQIRLVQDSFKSVAPIADQAATLFYGRLFELDPQLRKLFKS
                     DSKEQGRKLMTMIGMAVRGLDNIGALVPVVENLGRRHVAYGVMPGHFETVGAALLWTL
                     KQGLGSAFTSEVESAWAETYTLLANTMKEAATAQV"
     gene            complement(4739..5149)
                     /locus_tag="VGA59_01675"
     CDS             complement(4739..5149)
                     /locus_tag="VGA59_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718413.1"
                     /translation="MSALRKVSIVEARETWTPVVIIGSGRKASEGKAEPAAAPRPRLF
                     RNMALFLSAPFIGLLYAILLPFVGLAMLAWFAAKALYASAKVRKALRFGKRMSLIAAA
                     PLVGLVYLIVLPFAGLGMLAWFGATMLMAPAPAR"
     gene            complement(5646..6287)
                     /locus_tag="VGA59_01680"
     CDS             complement(5646..6287)
                     /locus_tag="VGA59_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX9718414.1"
                     /translation="MLPHTPLLLAFVGASLVLALTPGPAVVYIVARTLAQGRACGFAS
                     VLGVALGNLCNAVGAALGLAALFAVSSAAFTLVKWAGAAYLVYLGIRMWRAQPAMAHA
                     PPQVPVKPLRRVLRDGFLVALLNPKTTLFFAAFLPQFIDAHAGPLMIQTLALGGVFVG
                     IACCTDLVYVLTASLVASRLKQAARHAVWGNRLAGTSFIGLGVLAAMGTRPPR"
     gene            6591..6821
                     /locus_tag="VGA59_01685"
     CDS             6591..6821
                     /locus_tag="VGA59_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718415.1"
                     /translation="MFEPSTNQFQLCFQSLFDSGRGYAFPCDPQGRVDLDHLSERMRT
                     NYLFARAMVGRDLAVPAVEPGFQAAGGRVCSG"
     gene            6983..7159
                     /locus_tag="VGA59_01690"
     CDS             6983..7159
                     /locus_tag="VGA59_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718416.1"
                     /translation="MSATQDPAGSPVDPDDSPDGQVPPIPDEDGPHDVPDDKVIEKTL
                     PTTPMQDKGAGGPA"
     gene            7383..7622
                     /locus_tag="VGA59_01695"
     CDS             7383..7622
                     /locus_tag="VGA59_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX9718417.1"
                     /translation="MKTLATSICCAALVALTVPAWADIGRDEAAAAAQRVSGARVLSV
                     EKSESARRPVWRVKVVTAKGEVKVILIDAASGQAL"
     gene            7634..8305
                     /locus_tag="VGA59_01700"
     CDS             7634..8305
                     /locus_tag="VGA59_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9718418.1"
                     /translation="MRLLLIEDDAALRLGLARRLEADGYRVDQAGDGADGLFQAQEYP
                     VDLAIVDLGLPKLSGLSIVQRLRQAGSTTPILILTARGSWQDKVAGLEAGADDYLVKP
                     FEYPELAARVKALLRRSMKAASDVLKIGPITIDFPAQFARLNDAAMDLTTYEYRVLEY
                     LVRARPRVVSKQELSDYLYPHDEDRDSNVLEVLVGRLRRKLDPEGTLAPIETLRGRGY
                     RFTLE"
     gene            8302..9633
                     /locus_tag="VGA59_01705"
     CDS             8302..9633
                     /locus_tag="VGA59_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9718419.1"
                     /translation="MSLDSIRARLILGATLVLVAFMAVAGLAVQRAHEDSVRAAHFAR
                     LQSTVYVLLAGAELDAAGALVMPAAFPEPRLSLPGSGLYANIVNINRGEEWQSASTLG
                     VAPPFQRSAAVGQWRHDTAAGPAGAFFIVAYGVKWSGPAADAPLVLSVAEDKAGFDRE
                     AGAFKRTLWQWLGAAGVLLLASQILFLEWGLSPLQRVAREIRRVENGEQAKVEGRYPT
                     EISALTGNLNTLIEQERVRRTRYKEALSFLAHSLKNPLAVLRTALEGEPAQLPGTVAQ
                     QVARMDDIVGHQLSRAAASGAARFVPLLPLAPVAGRIRDSLAKIHAGKGLAFSVECPA
                     DLSWRIDEGDAFELLGNVMDNAAKWAGQRVTARIWREGGRLRIQVDDDGPGFSDTQSV
                     LQIHVRGDERVPGHGVGLAVVNELVASHHGELNLARSDLGGARVDITLPGA"
     gene            9713..10135
                     /locus_tag="VGA59_01710"
     CDS             9713..10135
                     /locus_tag="VGA59_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718420.1"
                     /translation="MKPLSIKFLSAAALALVSLGVASAAQARSDVYFSIGVQSPGMYV
                     RPAPVYVQPRPAYVAPIPAYAEYRPVYVTPRVEYRYDDEWRNRQGWRHGQARRHARLY
                     GPYGDLDRDGIRNQVDRDRDGDGARNRFDRFPDNPYRY"
     gene            10669..11064
                     /locus_tag="VGA59_01715"
     CDS             10669..11064
                     /locus_tag="VGA59_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718421.1"
                     /translation="MNRTLASALAITTTTTAAAVLAVAAIASGNAYADDITIDNTPFV
                     SSKSRAEVRAEVMEQSKALGSASSEWAMQQNEPARPNSAYTRKAAQDEYIASRNEVNA
                     LNAEDSGSSYFATLPRTADTGVIMAGPAR"
     gene            11153..11848
                     /locus_tag="VGA59_01720"
     CDS             11153..11848
                     /locus_tag="VGA59_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9718422.1"
                     /translation="MMRIRLLIACKDERRGLTLSGHFTQEPSNGIAGHTTQLATLAGA
                     IAAGRPDVLVLEHALCAEQPANRVVPALRQLSPATRILLLCEACSHELLVSFVGLGVS
                     GCLLTSDHPSLLAKAVRSVYAGDTWFARPALFQAIQTLVGAASVPRTPFEEGPLTSRE
                     EEIFRLIGRGLTNKEIARQLDISGHTVKTHLHHIYAKLHQSGRYKAVLSQRAGRGGVR
                     GREAGMRGLTGHP"
     gene            complement(11903..12682)
                     /locus_tag="VGA59_01725"
     CDS             complement(11903..12682)
                     /locus_tag="VGA59_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9718423.1"
                     /translation="MSTRANQIDIPVDGKHIAGTLVGPDTMVPGVMLVHGWDGSQEQY
                     IARAHEIATLGCICLTFDLRGHVRHHAQRETVSREDNLQDVLAAYDVLVGHPAVDPGA
                     IAIVGSSYGGYLAAVVSALRPVRWLALRVPAIYKDADWNLPKNQLGGDELVAYRHGTV
                     SSGENRALAACAAFRGDVLIVESEHDSMVPHPVIQNYMAACAQAQSLTYRMISGADHA
                     LSEKEWQQTYTSLLVNWMSEMVLGARAGKTGTGVDAGSNPA"
     gene            complement(12666..>13747)
                     /locus_tag="VGA59_01730"
     CDS             complement(12666..>13747)
                     /locus_tag="VGA59_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HEX9718424.1"
                     /translation="PGRHDHASEHERISLVEFARRLAALRGGDFGGWYEPSRPYPGHV
                     YFVPSCTLTGEEAAALGIHGPDDLFGGVVPHAFVATKAISHPLVAADAAALAGWNPDF
                     GASVGDAVLAGYTAFTPDDARLAGLRLLANGPLRIKPVRATGGHGQSVARDPAEFQRL
                     LDGMDPGEVILHGLVLEEDLSEVNTFSIGQVKVADLTASYFGIQRLTRNNQGEEVFGG
                     SDLTVVRGDMDALLALEPARAVRRAVELARRYDAAVHACYAGFFASRKNYDIVVGRDA
                     AGRPRSGVLEQSWRVGGATGPELAALEVFRREPGRQRVRAAGVEIYGDSAPPPPHAIV
                     HFHGTDPHVGRLTKYTVVEPDVHTR"
BASE COUNT         2402 a         4558 c         4505 g         2282 t
ORIGIN      
        1 atcctgatca agctccgttg ccccgataac gcccggcggc tcgccttcgt gtcgcagggg
       61 cacgatcaga gtcttcacga cttggttgcg acatgcaggc ggctggattg gcatcgatgc
      121 agcgccaggt aatactcgag cgcccggcca ttggcggcat aggccacaga ttcgattcgc
      181 agcggtgatc gcgttgagcc ctgttctcca tgacggcaga gctgtcaaag gcaaggcttg
      241 cggtcgactt gcctccaagc ttgcgccgcc tgcaaaaagc cattgaagag gtccccttgc
      301 acgtcgcgcg ccggaagcac acacgatgac gccccagcgg taccatgcag cttccagagc
      361 catccaccca tggacaaatc ggctgcacca gcgcaggacg gggccccgcc cactgtcaag
      421 cgcatgattg tccaaacggc gctgagtctc gccgaggagg ccggaagctg ggacgcggtc
      481 catgtccacg ccatcgcccg cgcggcggga atcagtctcg aggaactgcg gcggcacttt
      541 ggcgacaagg acgcgatcgc cgaaggtttt ttcgacatcg ccgacgaggc cctgctgtcc
      601 gtgtgcaaag aacccggctg gggcgaacag gaaactcgcg aacgcctgta ccgggcggtg
      661 atgagctggc tcgacgcgct cgcgccgcac cgcagcctgg cagtgggcat gctgggctac
      721 aagtttcatc cggagcacct gcatctgcag gcgcgcggcg tcgcgcgcat cagccgtacg
      781 gtgcagtgga tcagggaggt gtccatgctg ccctccatcg gctggcgccg ggatctggaa
      841 gaggcggtgc tgacgaccat ctacctgacc acgttctcat actggctgac cgacacgtcg
      901 cccggggccg aacgcacgcg cggctttctc cggcgcctgc tggccggggc ggaaaaagcg
      961 gcgctgtggc tcgggtcgag cgcctgaagc cctggctctg cccactcacc cggctggccc
     1021 cgggctggcc gtttcgccga gcggatcttc cgcgggcacc agcaccgcca gcaaatcgga
     1081 agtcgtgcgc aggatgaggt cggcgcccgt ccggcgcagt tcccccgaca aacgcggccg
     1141 ggccaaagct tgcgtatgat gctcagccca cactccttac cttacctgga acctaccgat
     1201 gaagaaatcc atctcgggcc tgctgcctgt cgcggcactc gccctggttg ccggcgccgc
     1261 catcgcagcc acccccgccg cggccatgcc cggcgccacc gaaacgctgc ccggcggcat
     1321 cgtcgtgaaa cacgccaagg tgggcaccgg cgccagtccg gtggctgcgg acaccgtcca
     1381 ggttcactac cgcggcaggc tggcggacgg caccgagttc gacagttcct acaagcgcgg
     1441 caagccggcc agttttcccc tgggccgcgt catcccctgc tggacgcagg gcctgcagaa
     1501 gatgaaggtc ggcggctccg cgacgctgac ctgcccgccg gacaccgcgt atggctcgcg
     1561 cggagccggc ggggcggttc cgcccaacgc aacgctgacg ttcgaggtgg aactcctggc
     1621 catcgacaag aagtcgtgaa cggggcccat ggatcgcatt gcgggcgatc cgtcagccct
     1681 gcccggcggc ggcacaggtg atgcgttcat cgatagaacg cttcgacctt gcccttcaac
     1741 ttgatcagca gcgggttccc cttgcgatcc agcgttttcc cggcgggaac cttgacccag
     1801 ccctcgctga ggcagtactc ctcgacatcc ttgcgttcat tgccgttgag ccggatgcca
     1861 acgccatgct cgaacacggc ggcaacatga tggggactgc gcgggtcgac cgacaggcgg
     1921 tcgggcagcg gcggcaattg agtgggttcg ttcatgccgc ctattgtcca cgccggtgga
     1981 cccggcctca atgcacgcgc acgtcgagac ggcgcgtcag gccttccaga tcggtgatgc
     2041 ggatgtggcg cttgtccact tccagcaggc gctgctgctg gaacgacgtg aaggttcggc
     2101 tgacggtctc cagcttcaag cccagataac tgccgatctc ggcacggctc atgcgcaggt
     2161 ggaactcgcg ctcggagtag ccgcgcgcgg catagcgctg cgacaggttg agcaggaaag
     2221 cggccagccg ctcttcggcg ctcatgctgc ccaggagcat catcaggctg tgctcgcgca
     2281 ggatctcccg gctcatcaga cgcgtgatca cgtgctgcat gccggtggtg ccggccgcca
     2341 ggtcgcacaa gtgggcaaac gggatcgcgc agagttcggt gtcttccagt gccatcgccg
     2401 cgctcgcgtg atgtccttgt gccgcgccgt cgagtcccac cagctcaccc gcgatgtgaa
     2461 aaccgctcac ctgctcgcgg ccatcggaca gcagcaggct ggacttcagg gtgccggagc
     2521 gcacggcgta gatgtactgg aagcggtcgc cttcccggta cagggtctgc cccgccttga
     2581 tcttgcggcg ggtgaacgtc atgtcgtcca gccgggtcag atccacgcca ttcaggccgc
     2641 agggaaggca tagctcacgc aggtgacagg tggagcactg ggtcttcagc tgcacgacgg
     2701 gagcggccga ggcggggact gccacttgcg tggcgggcat gaagcggact cggtgaacgg
     2761 cggtttcaag tgcggcagcg gtcatggcat ttctctttgg tgtggacgcg ggagtcaatg
     2821 ggattgtgtc gagatgtttt cgacggcggc gaccagctgg tccatttccg tcgtcttgtc
     2881 cagaaagcgc tgggcccctt cgcccaggta gcgcttgcgg taggccgcac cggcgttgtt
     2941 gctgaacacg acaaagccga ttccgggttc cgcctggtgc acggcccgca gcacctgcag
     3001 gccggtgcct ccgtcgagcc ggacatccag caccaccacg tcggggtggg cggccagaat
     3061 gccgtcgata caggcttgcg gcgtttcgcc gtggccaaca acgcttattt cacgctccga
     3121 cagcattgcg gcgacacggt cgcgaacgat cgccgagtcc tcggcgataa agactttcac
     3181 gggaacatgg ctgcctgtca tggccatgac tgtcgcagtc ttcgcccgcg gcgaacatcg
     3241 gcgggggacc aatctcaccc tcggacttgg cctgaacgcg aggctcctgc gttcctggga
     3301 gcctcggaat attccgagtg ccgggagttg cgctgttgcc ggcgccagct ggcggtagcg
     3361 gacctgcgcg acaggggcga acccaatcgc gcaacccgtt caaagccttt gcggccctgg
     3421 aaatgtcgcc tatcatgaaa tggcaaggct ggccgcaagc cggcagtcac gaggggttgc
     3481 gcctttcagg ggcgagcccg cggacccatc aggagttgca cgcaatggac accagtcatc
     3541 agacagcaga gtacgaggcc gcacttgccg aggagcggct tttgtggaca cagctcgagg
     3601 ggccgctaac ggacgtcaat gagcgcgtta aggcgtttgc gcgctggagc gcggcggcag
     3661 agcgcgccag ggccatgtcc atcagaatgc atgaccaggc aacagtcgcc gagccacagt
     3721 agctctcgcg ccgacgcctt tgccgacgag gctgcgccgc ggacgcacct ccagcatgtc
     3781 cgcatccggc cttgcgcagc ctgatcagca tgggggcgcc tgccctcggc tagacctgtg
     3841 cggtagcggc ctctttcatg gtgtttgcca gcagggtgta cgtttccgcc caagccgatt
     3901 cgacctccga ggtgaaagcc gaccccaggc cttgcttcaa cgtccacaac agggcggcac
     3961 caacggtctc gaagtggccg ggcattaccc cataggcgac gtgccggcgg cccagattct
     4021 ccacgaccgg caccagcgcg ccaatattgt cgagtccgcg cacggccatg ccgatcatcg
     4081 tcatcaactt gcgtccctgc tccttggaat cgctcttgaa cagctttcga agttgcgggt
     4141 caagctcgaa caggcgcccg taaaacaggg tggccgcttg atcggcaata ggcgccaccg
     4201 atttgaatga atcctgcacc aatcgaattt gaatcgtgtc catggtttct ctcactggtc
     4261 gagttgcaat tcagacggtt ctctgcaacc gtccggccgg caggttcggc gcctttcgcg
     4321 ccggggtgcg aggggggagt cctggggaac tcgcttcctc gggttcgagg atgcaagcat
     4381 cgggtgcgcg cgtatcgcgc cggcgtcacc tgtcagcgcc cgtgttgccg tcttgtaatc
     4441 ggcgtgtatc gcgcgggcat gcgcatgccg gtgcatgaac ggctgccggc gcgccgcgaa
     4501 cgcgatttgc cagcccggga tgccgaactc cccgccgcgc tccagcgctc gtgcgtacga
     4561 agattagggg cctgttcaga ctgtttttgc actggtaaaa cagtctgaac aggccctaac
     4621 cacgatgcgc cgccgggggc atgcgccgct ggaaatcagg tactgcttgt tcgagaggcg
     4681 ggcccggccg attcaactgg ccgcttgccc gccgttctcg cgaagccccg ggcgccaact
     4741 acctggcagg tgccggggcc atcagcatcg tggcaccgaa ccaggccagc attcccaacc
     4801 cggcgaacgg caacacgatc aggtacacca ggcccacaag aggagccgcg gcgatcagcg
     4861 acatccgctt gccgaagcgc agcgccttgc gcaccttggc cgatgcgtac aaggcttttg
     4921 cggcgaacca ggccagcatg gccaggccaa cgaagggcag caggatcgcg tagagcagtc
     4981 cgatgaacgg cgccgacagg aacagcgcca tgttcctgaa caggcggggc cggggggccg
     5041 cggccggctc ggcttttccc tcgctcgcct tgcggccaga cccgatgatg acgaccggag
     5101 tccaggtttc acgggcctcg acgattgata ctttgcgaag tgcgctcatg atgacctcct
     5161 acaaaaaagc ggttcgatcc cgcaaaccgg ccggtactcg atcaggaccc tggctttggt
     5221 gttcatgtcg aagctcggtg ggtttgtgct ctggactacg catcagtcgt gccagcatcg
     5281 cgcccgaatg aagttcgcgt ggtggatcaa tgacttaggc gcgcttggcg ctccacgcga
     5341 gcggcggaac cgctgttttt ctccggccga tcgaggctcg aagtacaccg ggcccatgca
     5401 tcccggccgg ccgaaagaaa aaaaccgttt gaattcaggc aattgcagcg ttttggcggt
     5461 gtcggaggtt tctatacacc tgtgccggca tcggcgccaa aggcggtcaa gccgttgcgc
     5521 gctcaaccta tcacatgcgc gaccggcaca cgcggattgc agcgcagcgc tctctcgcgc
     5581 aatctcgccc cctgatctcc atcaggccgc gccccggcat cttgatgcga tgccccagca
     5641 ccggctcagc gcggggggcg cgtgcccatg gccgcgagca cacccaagcc gatgaaggat
     5701 gtgcccgcga gccgattgcc ccagacggcg tgccgtgccg cctgcttcag gcgcgatgcg
     5761 acgaggcttg ccgtcagcac gtagacaagg tcggtgcaac aggcgatacc gacgaagacg
     5821 ccgccgagcg ccagcgtctg gatcatcagg gggccggcgt gcgcgtcgat gaattgcggc
     5881 agaaaggcag cgaagaacaa ggtggttttc gggttcagca gcgccaccag gaagccgtcg
     5941 cgcaagacgc gccgcaaggg ctttaccggc acctgcggtg gcgcgtgcgc catggccggc
     6001 tgcgcccgcc acatccggat gccgagatag accaggtaag cggcgccggc ccatttcacg
     6061 agggtgaaag cggccgatga cacggcgaac aaggcggcca gcccgagtgc ggcgcccacg
     6121 gcattgcaga ggttgcccag tgccacgccc agcaccgagg cgaaaccgca ggcgcgaccc
     6181 tgcgccaggg tgcgggccac gatgtagacg acggcggggc cgggcgtgag agccagcacc
     6241 aggctggcgc ccacgaaagc caggagcaaa ggcgtgtggg gaagcatagg aaatcccgat
     6301 cggcaagtat cgcccgcgcc attgtccagg aagtctgctt ccccgtagcc atgcgtgaca
     6361 gtcttcacgg gccctttact gcaggacgtc ctcgtcgctg ccgtgcgttc cgttgaagcg
     6421 gtgtgccccg caaaacgctt gcggttcgac ttcgggagac ttgcatgaag ttaaagaaat
     6481 ggcctagccg ggacgcggtg aatgcttagg ggtgcggcgc gatcctcgcc gaaacttcat
     6541 tccatggaat cggtgcatct cgcgcctgaa ctcccaggag agaactgaaa atgttcgagc
     6601 cctcaacgaa tcaattccag ctgtgcttcc agtccttgtt cgacagcggg cgcgggtacg
     6661 ccttcccgtg cgatccccag ggccgggtcg atctggacca cctcagcgaa cggatgcgca
     6721 ccaactacct gttcgcccgg gccatggtcg gccgtgacct ggccgttccc gccgttgagc
     6781 cagggtttca ggctgctggt ggtcgcgttt gcagtggctg ataccactaa agaccgtggt
     6841 ttccgttctg gcgcggctga tcagccagcc aactcgcgat ctgcttaagc aactgtccta
     6901 cgtcaacagc ggatgaactg ccatgtcctg accctcctgg gcggcgatgc tgggcacatg
     6961 tgagccactc aggagaaacc acatgagcgc aactcaggac ccggcgggca gcccggtcga
     7021 tcccgacgat tcgccggacg ggcaagttcc accgattccg gatgaagacg gtccgcacga
     7081 cgtgccggac gacaaggtca tcgagaagac attgcccacc acgccgatgc aggataaagg
     7141 cgccggcggc ccggcgtaac tgcgcgcgca ccgccggggg caagtggtca ccgggcggct
     7201 cgaccggctg ctcgcgctat tgggccgccg gctcccattc agcttgtgtt catgcggcgg
     7261 cgggcaagat gcggccatac cagcaccagg acagctccat gaaacggcgc ccaccttcag
     7321 gtgcgggttt cgatcgaccg ggtggtgcgg tagtagcatc gccgttgaaa aaccggaaca
     7381 ccatgaaaac actcgccaca tccatctgct gcgccgccct cgtggcgctc acggtccctg
     7441 cctgggcgga tatcggccgt gacgaggcgg ccgcggccgc acagcgggtg agcggcgctc
     7501 gcgtgctgtc cgtggagaag tccgaatccg cccgccgccc ggtgtggcgg gtcaaggtgg
     7561 tcaccgcgaa aggcgaggtc aaggtgatcc tgatcgacgc ggccagcgga caagccctct
     7621 agggacgtcc agcgtgcgcc tgctgctgat tgaagacgat gccgccctgc gcctgggcct
     7681 cgcgcgccgg ctcgaggccg acggctatcg ggtcgaccag gcgggggacg gcgccgacgg
     7741 cctgttccag gcgcaggagt acccggtcga cctggccatc gtcgacctgg gcttgcccaa
     7801 gctgagtggt ttgagcatcg tgcagcggct gcggcaagcc ggcagcacga cgccgatcct
     7861 gatcctgacg gcccgcggca gctggcagga caaagtcgcc gggctggagg cgggcgccga
     7921 cgactacctg gtcaagccct tcgagtatcc ggagctcgcc gcgcgcgtga aagcattgct
     7981 gcgccgctcg atgaaggccg cgtccgacgt gctcaagatc gggccgatca cgatcgattt
     8041 cccggcgcag tttgcccggc tgaacgacgc ggcgatggac ctgacgacgt acgaataccg
     8101 cgtgctggaa tacctcgtgc gtgcacgccc ccgggtggtg agcaagcagg agctttcgga
     8161 ctacctttac ccgcacgatg aagaccggga cagcaatgtg ctggaagtgc tggtcgggcg
     8221 cttgcgccgg aaactggacc ccgagggaac cctggcgccg atcgaaacgc tgcgcggacg
     8281 gggttaccgc ttcaccctcg aatgagtctc gactcgattc gcgcccgcct gatcctgggg
     8341 gccacgctgg tgctggtggc cttcatggcc gtggcgggac tggcggtaca acgtgcgcac
     8401 gaggacagcg tgcgggcggc ccatttcgca cgcttgcaga gcaccgttta cgtgttgctg
     8461 gccggagccg aactcgacgc ggccggcgcc ctggtcatgc ccgcggcctt tccggagccg
     8521 cgcctgtcgc tgccgggctc cggcttgtac gcgaacatcg tcaacatcaa ccgcggcgag
     8581 gaatggcaat ccgcgtcgac cctcggcgtg gcgccgccct tccagcgaag cgccgccgtc
     8641 ggccagtggc gccatgacac cgccgccggt ccggccggcg ccttcttcat cgtcgcctat
     8701 ggcgtcaagt ggtcgggccc cgccgcggat gcgccgctgg tgctgtccgt ggccgaggac
     8761 aaggcgggct tcgaccgtga ggccggcgcc ttcaagcgca cgttgtggca gtggctgggc
     8821 gccgccggcg tgttgctgct ggcgtcccag atacttttcc tggagtgggg gctgtcgccg
     8881 ctgcaacggg ttgcgcgcga gatccgccgt gtggagaacg gcgagcaggc caaggtggag
     8941 ggccgctatc ccaccgagat ttcggcgctc acgggcaact tgaatacctt gatcgagcag
     9001 gagcgcgtgc ggcggacgcg ctacaaggaa gcgctcagtt tcctggccca cagcctcaag
     9061 aacccgctgg ccgtgctgcg cacggcgctg gaaggcgagc cggcgcagct gcccggcacc
     9121 gtggcgcagc aggttgcgcg aatggatgac atcgtcgggc accagctgag ccgggcagca
     9181 gcgagcggcg ccgcgcggtt cgtacccctg ctcccccttg cgccggtagc cggccgcatc
     9241 cgcgactcgc tggcgaagat ccatgccggc aaaggcctgg ccttctcggt cgagtgcccc
     9301 gccgacctga gctggcgtat cgatgaagga gatgcgttcg agctgctcgg caacgtgatg
     9361 gacaacgccg ccaaatgggc cgggcagcgc gtgacggccc gcatctggcg cgagggtggc
     9421 cgcctgcgaa tccaggtcga tgacgacggg ccgggtttta gcgacacgca atcggtgctg
     9481 cagatccatg ttcgcggcga cgagcgcgtt cccggccatg gcgtcgggct cgccgtggtc
     9541 aacgagctgg tggccagcca ccacggcgaa ttgaacctgg cacggtccga cctgggcggc
     9601 gcccgggtgg acatcacgtt gccgggcgcc tgattttctt tttttgttca ggctgtgttc
     9661 atgcaggccg gcgcaacatg cgttccatgc caacccaccc aaggagaaac gcatgaaacc
     9721 cctttccatc aagttcctct cggccgccgc gctggcgctg gtctccctcg gcgtggcttc
     9781 ggccgcgcag gcccgcagcg acgtgtactt ctcgatcggc gtgcagtcgc ccgggatgta
     9841 cgtgcggccg gcccctgtct atgtgcagcc ccggcctgcc tacgtcgctc ccattccggc
     9901 gtatgccgaa taccggccgg tgtacgtgac gccgcgcgtc gagtatcgtt acgacgacga
     9961 gtggcgcaac cgccagggat ggcgccacgg acaggcgcgc cgccatgcgc gcctctacgg
    10021 cccttacggt gacctcgacc gcgacggcat caggaaccag gtcgaccgcg accgcgatgg
    10081 cgacggcgcc cgcaaccgct tcgaccgctt tccggacaat ccgtaccgct attgatgcga
    10141 ctgagcgaaa agcccggcag gaatcgccgg gctttttcgt tttgggggcc cggcaacttg
    10201 cgcggtccat acgtcattcg ccgttcatca caaattcacg ggatggcggc cccccggagc
    10261 ttgccgtgct ttgcctgccg gacatccacc ctaacgagtc acccccgtga ccgggggcgg
    10321 acctgtgtct tgggtgaact gcttcacaga aaagccgcgc ccgaattcac ctgaatcttc
    10381 aggaagaaat caccggcgcg cgcatcgcgt gtccgtcgcg ggatcaggct gcgccttttc
    10441 ggtgacgcac gtcacacgcc aagacgcgac ctcgatgcat gaatgcggga taggcaatgc
    10501 ggcaggcggc aagcaccatc actgcactcg ttcgacattc ttcatcttca cttgtcacga
    10561 gcccctgctc ccacggtgct tcgctcaggc ggctcaccgg ggccggacag aagggcattg
    10621 agcggcccgc tatgtcccgg atcgcaacct cgctcaaagg agatcgacat gaaccgcact
    10681 ctcgcatccg ccctcgcgat cacaaccacg accacggccg ctgccgtcct cgccgtcgcg
    10741 gcaattgcct cgggcaacgc ctacgcggac gacatcacca tcgacaacac accgttcgtc
    10801 agcagcaaga gccgcgccga agtgcgggcc gaagtcatgg agcaatccaa ggcgctggga
    10861 agcgcctcca gcgaatgggc gatgcaacag aacgagccgg cccggcccaa ctccgcctac
    10921 acgcgcaagg ccgcgcaaga cgaatacatc gcttcgcgaa acgaagtcaa cgcgctgaac
    10981 gccgaagaca gcggctcgtc ctatttcgcg acgctgccgc ggaccgcgga caccggcgtc
    11041 atcatggccg gcccggcgcg ctgaaagcga tccaggtcgc gcgctgcgtg ccctcgccag
    11101 ccgcgcgacc aggttgtcgt caacgcgagg caatgccggg gaagggaggc ccatgatgcg
    11161 gatcagactg ctgatagcgt gcaaggacga gcgccggggc ctcaccttga gcgggcattt
    11221 cacgcaagaa ccatcgaacg gcattgccgg ccatacaacg caactcgcga cgcttgcggg
    11281 ggcgatcgcg gcggggcggc cggacgtcct cgttctggaa catgccctgt gcgccgaaca
    11341 gcctgcgaac cgggtcgtac ccgcgctgcg gcaattgagc cccgcaaccc ggatcctgtt
    11401 gctctgcgaa gcctgctcgc acgagctgct ggtctctttc gtcgggctgg gcgtcagcgg
    11461 ctgcctgctc acatcggacc acccgtccct gctcgcgaag gccgtgcgct cggtatacgc
    11521 cggagacaca tggttcgctc gcccggccct gttccaggcc attcagacac tcgttggcgc
    11581 agcgtccgta ccgcggacac ctttcgagga agggccactc acaagccgcg aagaggagat
    11641 cttccgtctc atcggccggg gcctgaccaa caaggagatc gcgcgccaac tggacatcag
    11701 cggccacacg gtgaagacgc accttcacca catctatgcc aagcttcacc agagcggacg
    11761 ctacaaggcc gtgctttcgc aacgtgccgg gcggggcgga gtgcgcggca gagaagctgg
    11821 catgcggggc ttgaccgggc atccatgaat tcggggtcat ggattgcggc tcgaggccgc
    11881 aatgacaaat caggagagct tgtcatgcgg ggtttgaccc ggcatccacg cccgtgccgg
    11941 tcttgccggc gcgcgcgccg agcaccattt cactcatcca gttgacgagc agtgaagtgt
    12001 aggtctgctg ccactccttc tcggacaggg catgatcggc gcccgaaatc atccggtagg
    12061 tcaacgactg ggcttgcgcg caggcggcca tgtagttctg gatcaccgga tgcggcacca
    12121 tcgaatcgtg ctccgattcc acgatcagca cgtcgccgcg gaacgccgcg caggcggcga
    12181 gcgcgcggtt ttccccggat gacaccgtgc cgtggcggta ggcgaccagc tcgtcgccgc
    12241 cgagctggtt cttcgggagg ttccagtcgg catccttgta gatcgccggt acccgcagcg
    12301 cgagccagcg caccggccgc agcgcactca cgaccgccgc caggtagccg ccgtagctgc
    12361 tgccgacgat cgcaatcgca cccgggtcga ccgccgggtg gcccaccagc acgtcgtagg
    12421 cggcgagcac gtcttgaagg ttgtcttcac ggctgacggt ctcgcgctgg gcgtgatggc
    12481 gcacatggcc gcgcaggtcg aaagtcaggc agatgcagcc aagcgtcgcg atctcgtggg
    12541 cgcgggcgat gtactgctcc tggctgccgt cccagccgtg caccagcatc accccgggca
    12601 ccatcgtgtc gggcccgacc agcgtgccgg cgatgtgctt gccgtcgacc gggatatcga
    12661 tctgattagc gcgtgtggac atccggctcg accaccgtgt actttgtcaa gcgaccgacg
    12721 tgcggatcgg tgccgtggaa atggacgatc gcatgcggcg gcggcggcgc actgtcgccg
    12781 tagatctcga ctccggccgc gcgcacccgc tggcgcccgg gttccctgcg aaatacctcg
    12841 agcgcggcga gttcgggacc ggtcgcgcct ccgacacgcc aggattgttc cagcacgccc
    12901 gagcgcgggc ggcccgccgc atcgcgcccg accacgatgt cgtagttctt gcgggaggca
    12961 aagaaaccgg cgtagcaagc gtgcacggct gcgtcatagc gccgcgccag ttcgaccgcg
    13021 cggcgaaccg cgcgcgccgg ctcgagcgcc agcagcgcgt ccatgtcgcc gcgcacgacg
    13081 gtcaggtcgg agccgccgaa aacctcttcc ccctggttgt tgcgggtcag gcgctgaatg
    13141 ccgaagtagc tcgccgtgag atcggccacc ttgacctggc cgatgctgaa ggtgttgacc
    13201 tcgctgaggt cttcctcgag caccagcccg tgcaagatga cttcgcccgg atccatcccg
    13261 tctagcaggc gctgaaattc ggcgggatcg cgtgcaaccg actgaccgtg gccgcccgtc
    13321 gcgcgcaccg gcttgatgcg cagcggcccg ttcgcgagca gccgcagacc ggctagtcgc
    13381 gcatcgtccg gggtaaaggc cgtgtagccg gcgagcaccg catcgccgac actggcccca
    13441 aagtccgggt tccatccggc cagggccgcg gcgtcggcgg ccacgagcgg atggctgatc
    13501 gccttggtgg ccacaaaggc atggggtacg acaccgccga acagatcgtc cggtccatga
    13561 atgcccaggg ccgcggcttc ctcgccggtc aaggtgcagc tcggcacgaa gtaaacgtgt
    13621 cccgggtagg gcctcgacgg ctcgtaccag cccccgaagt caccccccct cagggcagcc
    13681 agccggcggg cgaactcgac cagcgagatg cgttcatgtt cgctcgcgtg gtcgtgccgg
    13741 ccggggg
//
LOCUS       DASWIY010000045        10184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2613164, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000045 DASWIY010000000
VERSION     DASWIY010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10184
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2613164"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..590
                     /gene="nuoE"
                     /locus_tag="VGA59_01735"
     CDS             81..590
                     /gene="nuoE"
                     /locus_tag="VGA59_01735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX9718425.1"
                     /translation="MADTSQYQISEATRALFDKEVAKYPADQKQSAVMACLAILQRER
                     GWISPETEQEVADYLGMPPIAVHEVTTFYNMYNGKPVGKYKLNVCTNLPCQLRDGARA
                     LRYLEHRLGIAMGETTRDGLFTLQQCECLGACADSPVLLVNDQTMCSFMSDDKLDQLV
                     DGLKKAEQE"
     gene            587..1981
                     /gene="nuoF"
                     /locus_tag="VGA59_01740"
     CDS             587..1981
                     /gene="nuoF"
                     /locus_tag="VGA59_01740"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX9718426.1"
                     /translation="MNAQQVLSQFQATGVQTCFHGRHINPQIYAGLDGTNWRLKDYEA
                     REGYAALRKMLGIGGGEAMTPDQVVATVKESALRGRGGAGFPTGLKWSFMPKQYTGPK
                     YVVCNSDEGEPGTCKDRDLLQFNPHMVIEGMIIAAYAMGTQVGYNYIHGEIFQTYQRF
                     EEALEEARAAGYLGDNILGSGFGFQLHACHGWGAYICGEETALLESVEGKKGQPRFKP
                     PFPASFGLYGKPTTVNNTETFAAVPWIIRNGGKAYVECGKPNNGGTKIYSMSGDVELP
                     GNYEVPMGTPFGTLLELAGGVRKGRRLKAVIPGGSSSPVLPADLMMACTMDYDSIAKA
                     GSMLGSGAVIVMDDTRCMVESLKRLSYFYMHESCGQCTPCREGTGWLWRVVDRIHNGH
                     GKPSDLDLLDNVADNIQGRTICALGDAAAMPVRAMLKHFRHEFEAMIAEAQRKTLTPT
                     LSQGERVSTEARSA"
     gene            1986..4196
                     /gene="nuoG"
                     /locus_tag="VGA59_01745"
     CDS             1986..4196
                     /gene="nuoG"
                     /locus_tag="VGA59_01745"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX9718427.1"
                     /translation="MIEIELDGQNVGVPEGSMIMHAAEKTGTYIPHFCYHKKLSIAAN
                     CRMCLVEVEKAPKPMPACATPVTKGMIVHTKSDKALKAQQSVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGATASRYDEEKRVVLHKDVGPLISMEEMSRCIHCTRCVRFGQEV
                     AGVMELGMIHRGEHSEITTVLGDTVDSEVSGNMIDLCPVGALTSKPFRYQARNWELSR
                     RKSVSPHDSTGANLIVQVKSNKVMRVLPLENEEVNECWLADRDRFSYEALNGDERLTR
                     PMLKQGGEWKIVDWQTALEYVANGLKQIKAEHGAKSIGALVSPHSTVEELCLAGSLVR
                     GLGSENIDYRLRNAQFAKAEGVRWLGTSIASLSQLQRALVIGSNLRKDHPLFAVRVRA
                     AVRKGAAVSLIHDVANDWAMSIATSVIAPAANWAQALADVAAAVAAEKGAAAPAQGRA
                     TDAAKSIAKSLLGGERKAILLGNAAAHHANASTLLALANWIGEQTGATVGYLTEAANT
                     VGAQLAGALPGAGGLNAGQMLNGALKAAILLNNEPMFDSAAGTKAPAALGQAEMCVTL
                     SPFKANLEFSDVLLPITPFTETSGTFVNAEGRVQSFHAVVKPLGEARPAWKVLRVLAN
                     LLGLPGFEFESAQEVLVAARGAQDAQAALVQGGKLGNATSAAIDLSSAAGNPVTAAIY
                     ELDSIVRRAPSLQLTADARAARVQMEAGAEAPALGARVPSGGSSVPASVGALSS"
     gene            4193..5269
                     /gene="nuoH"
                     /locus_tag="VGA59_01750"
     CDS             4193..5269
                     /gene="nuoH"
                     /locus_tag="VGA59_01750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX9718428.1"
                     /translation="MIDQIYGFGNGLINAGWWTGAAWPTTWALIKIVVLVAPLMGAVA
                     YLTLWERKAISFTQIRIGPNRIGPSGLLQPIADAVKLMTKEIILPTMANKGLFYLGPI
                     MTIMPALVAWVVIPFGPEVAVANVNAGLLLLMAITSMEVYGVIIAGWASNSKYAFLGA
                     LRASAQMVSYEIAMGFCLVVVLMVAGSMNMTDIVMGQGKGIGVSAGLNFLSWNWLPLL
                     PIFFVYFISGLAECNRHPFDVVEGESEIVAGHMIEYSGMSFGLFFLAEYANMWLISIL
                     AVTMFLGGWLPPFEFLGFIPGWIWLGIKTFILVTMFLWVRASFPRFRYDQIMRLGWKI
                     FIPVTLVWLVVVGAWMQTPFSIWK"
     gene            5290..5814
                     /gene="nuoI"
                     /locus_tag="VGA59_01755"
     CDS             5290..5814
                     /gene="nuoI"
                     /locus_tag="VGA59_01755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX9718429.1"
                     /translation="MSTQAYAPQAAPGFSLKDFLSSFMLIELFKGLAVTGRYAFKRKI
                     TIQFPEEKTPLSPRFRGLHALRRYENGEERCIACKLCEAVCPAMAITIESDQRADGTR
                     RTTRYDIDLTKCIFCGFCEESCPVDSIVETSILEYHGEKRSDLYYTKDMLLAVGDRYE
                     AEIAAMKAADAKYR"
     gene            5846..6499
                     /locus_tag="VGA59_01760"
     CDS             5846..6499
                     /locus_tag="VGA59_01760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX9718430.1"
                     /translation="MDIRTGFFYLFSAILLFAAFRVITARNPVYAVLFLVLAFFQAAA
                     VWILLRAEFLAITLVLVYVGAVMVLFLFVVMMLDINVDSLRAGFWKHFPLAATIGVVI
                     ALEMAAVLMGGFRMGEEPRSVPVQAAVQGAAQYSNTKELGKLLYSQYLYPLQIAAVLL
                     LVAIIAAIALTLRQRKDTKKTNPSEQVRVRARDRLRIVQIAPTRVAAPGGAAAEPKP"
     gene            6496..6804
                     /gene="nuoK"
                     /locus_tag="VGA59_01765"
     CDS             6496..6804
                     /gene="nuoK"
                     /locus_tag="VGA59_01765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX9718431.1"
                     /translation="MTLTLGHFLSLGAMLFALAVIGIFLNRRNLIVLLMAIELMLLAV
                     NMNFVAFSHYLGDMHGQVFVFFILTVAAAESAIGLAILVLLFRNKSNINVDELNTLKG
                     "
     gene            6824..8866
                     /gene="nuoL"
                     /locus_tag="VGA59_01770"
     CDS             6824..8866
                     /gene="nuoL"
                     /locus_tag="VGA59_01770"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX9718432.1"
                     /translation="MSQTLNASTLLAVPLAPLAGALLAGIFGTTFGGNWIGRRLSHTL
                     CILGVFVAFVISAMTLKSVALDGARFNGTIYEWMVIGGLKMEIGFLIDGLTAMMMCVV
                     SFVSLMVHIYTIGYMEEDDGYNRFFAYISMFTFSMLMLVMSNNFLQLFFGWEAVGLMS
                     YLLIGFWYNRPTAIFANLKAFIVNRVGDFGFILGIGLIAAYAGTLSYVDAFAKGGELA
                     RLALPGTSWALITVICICLFIGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTA
                     GIFMVARMSPLFELSDVALSFVMTIGAITALFMGFLGIIQNDIKRVVAYSTLSQLGYM
                     TVALGASAYSVGVFHLMTHAFFKALLFLAAGSVIIGMHHNQDIRWMGGVRKYMPITWI
                     TALLGSLALIGTPLFAGFYSKDSIIEAVHFSTLPGAGFAYFAVMAGVFVTAFYSFRMY
                     FLVFHGKERFDQNPDAHHGHGGHGHHEEKHVPHESPWVVTAPLVLLAFPSVVIGFLTI
                     EPMLFGNLLKDSIIVDAARHPAMAALAKEFHGPVQMALHGLTTLPFWLALAGVVSAWY
                     LYLMNPALPAKIKRTLQPLYTVLENKYYMDWFNENVLARGARLLGVGLWKGGDEGVID
                     GAMVNGSARGVVWVAGIVRWFQTGYIYHYAFGMLLGVILLMSYFVSWPMLVEWLKR"
     gene            8886..>10184
                     /locus_tag="VGA59_01775"
     CDS             8886..>10184
                     /locus_tag="VGA59_01775"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX9718433.1"
                     /translation="MGLLSLAIWTPIFFGAVLLALGRDDQARAVRWFALIGAIASLLV
                     TLPLYPAFDTASSAMQFVEKAPWIERFNVFYHVGLDGISFWFVLLTAFINLVVIISAW
                     ESITERVNQYMGAFLILSGFMIGVFCALDGVLFYVFFEATLIPMYLIIGIWGGPNKIY
                     AAFKFFLYTLLGSLLMLVALVYLYTKSGGSFDIATWHKLPLSLSAQTLLFFAFFAAFA
                     VKVPMWPVHTWLPDVHVEAPTGGSAVLAAIMLKLGAYGFLRFSMPIAPDASHEWAWLI
                     IGLSLIAVVYVGLVAMVQQDMKKLVAYSSVAHMGFVTLGFFIFNDIGVAGGIVQMIAH
                     GFVSAAMFLCIGVLYDRVHSRQIASYGGVVNTMPNFSAFAMLFAMANCGLPATAGFVG
                     EWMVILGAVRANFMLGLAAASALIFGAAYTLWMYKRVYLGP"
BASE COUNT         1758 a         3272 c         3262 g         1892 t
ORIGIN      
        1 ctcccccaca agggggcgcc accagcggcc cggcaaagcc ggttccgcgg tggccctgga
       61 aaaagccgtg gagaaccaaa atggctgaca catctcagta ccaaatttct gaagctacga
      121 gggccctctt cgacaaggaa gtggccaagt accccgccga ccagaagcag tccgccgtga
      181 tggcctgtct ggcgatcctg cagcgggagc ggggctggat cagccccgaa accgaacagg
      241 aagtggccga ctacctgggc atgccgccga tcgcggtgca cgaggtcacc actttctaca
      301 acatgtacaa cggcaagccg gtgggcaagt acaagctcaa cgtctgcacc aacctgccgt
      361 gccagctgcg cgacggcgcc agggccttgc gctacctgga gcaccggttg ggcatcgcga
      421 tgggcgagac cacgcgggat ggcctgttca ccctgcagca gtgcgagtgc ctgggcgcct
      481 gcgcggattc gccggtgttg ctggtgaacg accagacgat gtgcagcttc atgagcgacg
      541 acaagctcga ccagctggtc gacggcctga agaaggcgga gcaggaatga acgcgcaaca
      601 agtgctttcg cagttccagg ccacgggggt ccagacctgt ttccatggac gccacatcaa
      661 tccccagatc tacgcgggcc tggacggcac caactggcgg ctgaaggatt acgaggcgcg
      721 cgagggctac gccgcgctgc gcaagatgtt gggcataggc ggcggcgagg cgatgacgcc
      781 cgaccaggtc gtcgccaccg tcaaggagtc tgccttgcgc ggccgcggcg gcgccggctt
      841 ccccacgggc ctgaagtgga gcttcatgcc caagcagtac accgggccga aatatgtggt
      901 gtgcaattcg gacgaaggcg agccgggcac ctgcaaggac agggacctgc tgcagttcaa
      961 cccgcacatg gtgatcgagg gcatgatcat cgcggcctac gcgatgggca cgcaggtcgg
     1021 ctacaactac atccacggcg agatcttcca gacctaccag cggttcgagg aagcgctgga
     1081 agaggcgagg gcggccggct acctgggcga caacatcctg ggttcgggtt tcggcttcca
     1141 gctgcatgcc tgtcacggct ggggcgccta catctgcggc gaggaaaccg cgctactcga
     1201 atccgtcgaa ggcaagaagg gccagccgcg cttcaagccg ccgttcccgg cgagcttcgg
     1261 cctgtacggc aagccgacca cggtcaacaa caccgagacc ttcgctgccg tgccctggat
     1321 catccgcaat ggcgggaagg cctatgtcga atgcggcaag ccgaacaacg gcggcaccaa
     1381 gatctactcg atgtcgggcg acgtggaact gccgggcaac tacgaagtgc cgatgggcac
     1441 gccgttcggc acgctgctgg aactggccgg cggggtacgc aagggacgca ggctcaaggc
     1501 cgtgatcccc ggcggttcgt cgtcgcccgt gctgcccgcc gacctcatga tggcgtgcac
     1561 gatggactac gactccatcg ccaaggccgg ttccatgctc ggatccggcg ccgtgatcgt
     1621 gatggacgac acccgctgca tggtcgaatc gctcaagcgc ctgtcgtact tctacatgca
     1681 cgagtcctgc ggccaatgca ccccctgccg cgagggcacg ggctggctct ggcgcgtggt
     1741 cgaccgcatc cacaacgggc acggcaagcc cagcgacctc gacctgctcg ataacgtcgc
     1801 cgacaacatc cagggccgca ccatctgcgc cctgggcgat gcggcggcca tgcccgtgcg
     1861 cgcgatgctg aagcatttcc gccacgagtt cgaggccatg atcgccgaag cccaaaggaa
     1921 gaccctcacc ccgaccctct cccaagggga gagggtgagt acggaagcgc ggagcgctta
     1981 atcacatgat tgaaatcgaa ctagacggtc agaacgtggg agtccccgaa ggcagcatga
     2041 tcatgcatgc ggccgaaaaa acgggcacct acatccctca cttctgctac cacaagaagc
     2101 tatccatcgc ggccaactgc cgcatgtgcc tggtcgaggt ggaaaaggcg cccaagccga
     2161 tgccggcctg cgccacgcct gtcaccaagg gcatgatcgt ccacaccaaa agcgacaagg
     2221 ccctgaaggc ccagcagtcg gtcatggagt tcctgctgat caaccatccg ctggactgcc
     2281 cgatctgcga ccagggcggc gagtgccagc tgcaggacct ggccgtgggc tacggcgcaa
     2341 cggcatcgcg ctacgacgag gaaaagcgcg tcgtgctgca caaggacgtg ggccccctga
     2401 tctcgatgga ggagatgagc cgctgcatcc actgcacgcg ctgcgtgcgt ttcggccagg
     2461 aagtggccgg ggtgatggag ctgggcatga tccaccgggg cgagcactcc gaaatcacca
     2521 ccgtgctcgg cgacaccgtg gactccgagg tttcgggcaa catgatcgac ctgtgcccgg
     2581 tcggcgccct gacgagcaag cccttccgct accaggcccg caactgggag ctgtcgcgcc
     2641 gcaagtccgt gagcccccac gattccaccg gcgccaacct gatcgtccag gtcaagagca
     2701 acaaggtcat gcgcgtcctg cccctcgaaa acgaggaggt caacgaatgc tggctggccg
     2761 accgcgaccg cttctcgtac gaagcgctca acggcgacga gcgcctgacc cgccccatgc
     2821 tcaagcaggg cggcgaatgg aagatcgtgg actggcagac cgcgctggaa tacgtggcca
     2881 atggcctgaa acagatcaag gccgagcatg gcgcgaaaag catcggcgcg ctcgtcagcc
     2941 cgcacagcac cgtggaagag ctgtgtctcg ccggttccct ggtacgcggt ctcggcagcg
     3001 agaacatcga ttaccgcttg cgcaatgcgc agttcgccaa ggctgaaggg gtccgctggc
     3061 tgggcacctc catcgcttcg ttgtcgcagc tgcagcgcgc gctggtgatc ggttcgaacc
     3121 tgcgcaagga ccatccgctg ttcgccgtgc gcgtgcgcgc ggcggtgcgc aagggcgcgg
     3181 ccgtgtcgtt gatccatgac gtcgccaacg attgggcgat gtcgattgcc accagcgtca
     3241 tcgcgcccgc cgccaattgg gcgcaggcgc tggccgacgt ggccgctgcc gtggcggccg
     3301 agaagggcgc cgcggcgccg gcccaaggca gggccaccga cgccgcgaag tcgatcgcga
     3361 agtcgctgct cggcggcgaa cgcaaggcga tcctcctggg caatgccgct gcgcatcacg
     3421 ccaatgcctc gacgctgctg gcgctggcga actggatcgg tgagcagacc ggtgcgaccg
     3481 tgggctatct gaccgaggcg gccaatacgg ttggcgcaca gctggccggc gcgctgcccg
     3541 gtgccggcgg cctgaatgcg ggccagatgc tgaacggcgc cctgaaggcc gcgatcctgc
     3601 tgaacaacga acccatgttc gactccgctg cgggcaccaa ggctccagcc gcgctcggcc
     3661 aggccgaaat gtgcgtgacc ctgagcccgt tcaaggccaa cctggaattc agcgacgtgc
     3721 tgttgcccat cacgccattc acggaaacgt cgggcacgtt cgtcaatgcc gagggccggg
     3781 tgcaaagctt ccatgcggtg gtcaagcctc tgggcgaagc gcggcccgcc tggaaggtgt
     3841 tgcgcgtgct ggccaacctg ctgggcctgc cgggcttcga gttcgagtcc gcgcaggaag
     3901 tcctggtggc ggctcgcggc gcgcaggatg cgcaggccgc cctcgtacaa ggcggcaagc
     3961 tgggcaacgc gacgtccgcc gccatcgacc tgtcgagcgc ggcaggcaat ccggtgaccg
     4021 ccgcgatcta cgagctcgac agcatcgtcc ggcgcgcgcc gtcgctgcag ctgacggccg
     4081 atgcgcgtgc ggcaagagtg caaatggagg ctggcgccga agcgccggcc ctgggcgcga
     4141 gagtcccgtc ggggggcagc tcggtgccgg caagcgtggg agccctgtca tcatgatcga
     4201 ccagatctac ggcttcggaa acggcctgat caacgccggc tggtggaccg gggctgcgtg
     4261 gcccacgacc tgggcgctga tcaagatcgt cgtcctcgtg gcgccgctga tgggcgccgt
     4321 cgcctacctc acgctctggg agcgcaaggc gatcagcttc acgcagatcc gcatcggccc
     4381 caaccgcatc ggcccctccg gactgctgca gcccatcgcg gacgcggtga agctgatgac
     4441 caaggaaatc atcctgccca cgatggccaa caagggcctg ttctacctgg gcccgatcat
     4501 gaccatcatg ccggcgctgg tggcttgggt cgtgatcccc ttcggtcccg aggtcgccgt
     4561 ggccaacgtc aatgccggcc tgctgctgct gatggcgatc acctccatgg aggtgtatgg
     4621 cgtgatcatc gccggctggg cgtccaactc gaagtacgcc ttcctcggcg cgctgcgcgc
     4681 ctcggcccag atggtgagct acgagatcgc gatgggcttc tgcctggtgg tcgtgctgat
     4741 ggtcgccggc agcatgaaca tgacggacat cgtcatgggc cagggcaagg gaataggcgt
     4801 gagcgcgggc ctgaatttcc tgtcgtggaa ctggctgccc ctgttgccga tcttctttgt
     4861 ctacttcatc tcgggcctgg ccgaatgcaa ccgccacccg ttcgacgtgg tcgaaggcga
     4921 atcggaaatc gtcgcgggcc acatgatcga gtactcgggc atgagcttcg gcctgttctt
     4981 cctggccgag tacgccaata tgtggctgat ctcgatcctc gcggtgacca tgttcctggg
     5041 cggctggctg cctccgttcg agttcctcgg cttcatcccg gggtggatct ggctgggcat
     5101 caagaccttc atcctcgtca ccatgttcct gtgggttcgt gcctcgttcc cgcgcttccg
     5161 ctacgaccag atcatgcgcc tgggctggaa gatcttcatt ccggtcacgc tggtctggct
     5221 ggtggtggtg ggcgcctgga tgcagacccc gttcagtatc tggaagtaga aacgaagaag
     5281 tatcccgcca tgtctaccca agcatacgca ccgcaagccg cccccggctt ctcgctgaag
     5341 gacttcctgt ccagcttcat gctgatcgag ctgttcaagg gcctcgccgt caccggcaga
     5401 tacgctttca agcgcaagat cacgatccag ttccccgagg agaagacgcc gctgtccccg
     5461 cgcttccgcg gcctgcacgc gctgcgccgc tacgagaacg gcgaagagcg ttgcatcgcg
     5521 tgcaagttgt gcgaggccgt gtgcccggcg atggcgatca cgatcgagtc cgaccagcgc
     5581 gccgacggca cgcgccgcac gacccgctac gacattgacc tgacgaaatg catcttctgc
     5641 ggcttctgcg aggaaagctg cccggtggat tcgatcgtgg agacctcgat cctggagtac
     5701 cacggcgaaa agcgcagcga cctgtactac accaaggaca tgttgctggc cgtcggcgac
     5761 cgctacgagg ccgagattgc cgcaatgaag gcggccgacg ccaaataccg ctgagccgcg
     5821 gcgaagaccg aaccaaagaa cgaccatgga tatccgaacc ggctttttct acctgttctc
     5881 cgcaatattg ctgttcgcgg cgtttcgtgt gatcacggcc cgcaatccgg tgtatgcggt
     5941 gctgttcctc gtgctggcgt ttttccaggc tgcggccgtg tggatcctgc tgcgggccga
     6001 gttcctcgcc atcacgctgg tgctggtgta tgtgggggcc gtgatggtgc tgttcctgtt
     6061 cgtcgtgatg atgctggaca tcaacgtcga cagcttgcgg gccggattct ggaagcactt
     6121 tcccctggcg gccaccatcg gcgtggtgat tgcgctggag atggcggctg tgctgatggg
     6181 cggcttccgg atgggagagg agccccggtc ggtgccggtg caggcggcgg tgcagggcgc
     6241 ggcccagtac tccaacacca aggaactggg caagctgctg tacagccagt acctgtaccc
     6301 gctgcagatc gccgccgtgc tcctgttggt ggcgatcatc gcggccatcg cattgacact
     6361 gcgccagcgc aaggacacca agaagaccaa tccctccgaa caggtgcgag tcagggcgcg
     6421 agatcgcctg cgcatcgtcc agatcgcccc cacccgcgtg gccgctcccg gcggcgccgc
     6481 cgcggagccc aaaccatgac actcactctc ggacattttc tttcgctcgg cgccatgctg
     6541 ttcgcgctgg cggtgatcgg catcttcctg aaccgccgca acctgatcgt gctgctgatg
     6601 gccatcgagc tgatgctgct cgcggtgaac atgaacttcg tcgccttctc ccattacctg
     6661 ggcgacatgc acgggcaggt gttcgtgttc ttcatcctga cggtggcggc ggcggagtcg
     6721 gccatcgggc tggcgatcct ggtcctgctg ttccgcaaca agtccaacat caacgtcgac
     6781 gaactcaaca cgctgaaggg ctgatttcgg cgccaacaca accatgagcc aaaccctcaa
     6841 cgcttccacc ctgctcgccg ttccgctggc gcccctggcc ggcgccctgc tggcggggat
     6901 cttcggcacc accttcggcg gcaactggat cggccggcgc ctgtcgcaca ccctgtgcat
     6961 cctgggcgtg ttcgtggcct tcgtgatttc ggcgatgacg ctcaagagcg tggcgctgga
     7021 cggcgcccgc ttcaacggga ccatctacga gtggatggtg atcggcggcc tgaagatgga
     7081 aatcggcttc ctcatcgacg gcctcacggc catgatgatg tgcgtggtga gcttcgtgtc
     7141 gctgatggtc cacatctaca ccatcggcta tatggaagag gacgacggct acaaccgctt
     7201 cttcgcctac atctcgatgt tcaccttctc gatgctgatg ctcgtgatga gcaacaactt
     7261 cctgcagctg ttcttcggtt gggaggcggt gggcctgatg tcctacctgc tgatcggttt
     7321 ctggtacaac cgcccgaccg ccatcttcgc caacctgaag gccttcatag tcaaccgcgt
     7381 cggcgacttc ggcttcatcc tgggcatcgg gctcatcgcc gcctacgccg gcaccctgag
     7441 ctacgtggac gcgttcgcca agggcggcga actcgccagg ctcgccttgc ccggaaccag
     7501 ctgggcgctg atcacggtga tctgcatctg cctgttcatc ggcgccatgg gcaagagcgc
     7561 ccagttcccg ctgcacgtct ggctgcccga ctccatggaa ggcccgacgc cgatctcggc
     7621 cctgatccac gccgcgacga tggtgacggc gggcatcttc atggtggcgc gcatgtcgcc
     7681 gttgttcgag ctgagcgatg tggcgctgtc gttcgtgatg acgatcggcg cgatcacggc
     7741 cctgttcatg ggctttctgg gcatcatcca gaacgatatc aagcgggtgg tggcgtactc
     7801 gacgctttcc cagctcggct acatgaccgt ggcgctcggc gcgtcggctt actcggtggg
     7861 tgtgttccac ctgatgaccc acgccttctt caaggccctg ctgttcctgg cagccggctc
     7921 cgtgatcatc ggcatgcacc acaaccagga catccgctgg atgggcggcg tgcgcaagta
     7981 catgcccatc acctggatca ctgcgctgct ggggtcgctg gcgctgattg gcacaccgct
     8041 gttcgcgggc ttctactcga aggacagcat catcgaggcc gtgcacttca gcacgctgcc
     8101 gggcgccggc ttcgcgtact ttgcggtgat ggcgggcgtt ttcgtgacgg ccttctactc
     8161 gttccgcatg tatttcctgg tgttccacgg caaggagcgc ttcgaccaga atcccgacgc
     8221 ccatcacggg cacggcgggc atggccacca cgaagagaaa catgtgccgc acgagtcgcc
     8281 ctgggtggtg acggcgccat tggtgctgct ggcctttccg tccgtggtga tcggcttcct
     8341 cacgatcgag ccgatgctgt tcggcaacct gctcaaggat tcgatcatcg tcgatgccgc
     8401 caggcatccc gccatggcgg ccctggcgaa agaattccac gggccggtgc agatggcttt
     8461 gcacggcctg actaccttgc ccttctggct ggcgctggcc ggcgtggtgt cggcctggta
     8521 cctgtacctc atgaacccgg ccctgccggc gaagatcaag agaaccttgc agccgctgta
     8581 caccgtcctc gagaacaagt actacatgga ctggttcaac gagaacgtgc tggcccgcgg
     8641 cgcgcggctc ctgggcgtgg gcctgtggaa gggcggcgac gagggcgtga tcgacggcgc
     8701 gatggtcaac ggcagcgcgc gtggtgtggt gtgggtcgcc ggtatcgtga ggtggttcca
     8761 gaccggctac atctatcact acgcgttcgg gatgttgctg ggcgtgatct tgctgatgag
     8821 ttacttcgtc agttggccga tgttggtcga atggctcaaa cgataaggca ggaataggaa
     8881 agaaaatggg actcttgagc ctcgccatct ggacaccgat cttcttcggc gccgtcctgc
     8941 tcgcgctggg tcgcgatgat caagcgcgcg cggtgcgctg gttcgcgttg atcggcgcca
     9001 tcgccagcct gctggtcaca ctgccgctgt accccgcctt cgacacagcg tcctcggcga
     9061 tgcagttcgt cgaaaaggcg ccctggatcg agcgcttcaa cgtcttctat cacgtcggcc
     9121 tcgacggcat ctcgttctgg ttcgtgttgc tgacggcctt catcaacctg gtcgtgatca
     9181 tctcggcatg ggaatcgatc accgagcgcg tcaaccagta catgggtgca ttcctgatcc
     9241 tgtcggggtt catgatcggc gtgttctgcg cgctcgacgg cgtcctgttc tacgtattct
     9301 tcgaggcgac gctgatcccg atgtacctca tcatcggcat ctggggcggc cccaacaaga
     9361 tctacgctgc cttcaagttc ttcctgtaca cgcttctggg ctcgctgctg atgctggtcg
     9421 cgctggtgta cctgtacacc aagtcggggg gcagcttcga catcgccacc tggcacaagc
     9481 tgcccctgag cctctcggcg cagacgctgc tgttcttcgc cttcttcgcc gctttcgcag
     9541 tcaaggttcc gatgtggccc gtccacacct ggctgcccga cgtgcacgtt gaggcgccca
     9601 ccggcggctc ggcggtgctg gcggccatca tgctgaagct gggcgcttac ggtttcctgc
     9661 ggttctccat gccgatcgcg cccgacgcat ctcatgaatg ggcctggctg atcatcggcc
     9721 tgtccctcat cgccgtcgtg tacgtgggac tggtggccat ggtgcagcag gacatgaaga
     9781 agctggtcgc ctattcgtcc gtggcgcaca tgggcttcgt cacgctgggc ttcttcatct
     9841 tcaacgacat cggcgtggcc ggcggcatcg tccagatgat cgcgcacggc ttcgtgtcgg
     9901 ccgccatgtt cctttgcatc ggcgtgctgt acgaccgggt ccattcgcgc cagatcgcca
     9961 gctatggcgg ggtggtgaac accatgccca acttctcggc gttcgcgatg ctgttcgcca
    10021 tggccaactg cggccttccg gcgacggccg ggttcgtggg cgaatggatg gtgatcctgg
    10081 gggcggtcag ggccaacttc atgctggggc tggccgccgc gtcggcgctg atcttcggcg
    10141 cggcgtacac gctgtggatg tacaagcggg tttacctcgg gccg
//
LOCUS       DASWIY010000046        20803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1505318, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000046 DASWIY010000000
VERSION     DASWIY010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 20803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20803
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505318"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3039)
                     /locus_tag="VGA59_01780"
     CDS             complement(<1..3039)
                     /locus_tag="VGA59_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX9718434.1"
                     /translation="MAQRISIRGARTHNLKNIDLDIPRNQLVVITGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLQLMDKPDVDVIEGLSPAISIEQKATSHNPRSTVGTV
                     TEIHDYLRLLFARAGTPYCPNHDIPLQSQTVSQMVDAVLALPAESRLMVLAPVARDRK
                     GEFLDVFAEMQARGYVRFRVDGQTYEFDALPRLKKAEKHDIDVVIDRLKVRPDPGSGP
                     GAGSQQRLAESFEAALRLADGRAIAVEMDSGREHWFNAKFACPICNHSITELEPRLFS
                     FNSPVGACPTCDGLGHREFFDPARVVAFPSLSLASGAIKGWDRRNAYYFALLESLARH
                     YKFSLEVPFEELAPPVQQAILHGSGDEEIRFSYTLDSGSTSPGRPKTANAPSGGSEAW
                     GGRSSAGRRITKKHTFEGVIPNRERRYRETDSVMVREDLARYRSLQPCPDCRGTRLRS
                     EARHVKIGEGGEARAIYEIGHATLRESFEYFDGLKLRGAKAEIAEKVIREIGLRLKFL
                     NDVGLNYLSLDRSAETLSGGEAQRIRLASQIGSGLTGVMYVLDEPSIGLHQRDNDRLI
                     GTLRHLRDIGNSVIVVEHDEDMIRAADHLIDMGLGAGIHGGRVMAQGSYEDIKATAAS
                     LTGQYLSGKKSIPVPARRTRWLPVLDLIPAEMRKSSRFPPSDAALRRAAREAEHLATR
                     GVVQEIRVLGASGNNLKNINAAFPVGLFTCVTGVSGSGKSTLVNDTLYAAVARTLYRA
                     HEEPAPHEAVEGIEHFDKVINVDQSPIGRTPRSNPATYTGVFTPIRELMAEVSMAKER
                     GYGPGRFSFNVAGGRCEACQGDGVVKVEMHFLPDVYVACDVCHGQRYNRETLEVQWKG
                     RNIAQILDMTVEDAHLFLKPVPTIARKLQTLLDVGLSYIKLGQAATTLSGGEAQRVKL
                     ALELSKRDTGRTLYILDEPTTGLHFADIALLLRVLHQLRDAGNTIVVIEHNLDVIKTA
                     DWIVDLGPEGGDGGGRIVAQGTPQQVAQHPDSHTGRYLRQA"
     gene            3364..3831
                     /locus_tag="VGA59_01785"
     CDS             3364..3831
                     /locus_tag="VGA59_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718435.1"
                     /translation="MKNTLSACLLSLASLAPLTAAAIEPLVRFEDGIGVQPLRSGPAP
                     NVVFGVNPGGLPWVISRLSADVSTDGRIRVDGRGLLFAGGDNIGRTGGQSVRARLICA
                     GVYHDSDLVPLDARGDFTIGGFLNPFPPSPCATPVLLIVNAGGNWFAAGIPKP"
     gene            4315..4974
                     /locus_tag="VGA59_01790"
     CDS             4315..4974
                     /locus_tag="VGA59_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX9718436.1"
                     /translation="MPRFTVLKPNDLQVLEQFFLAWTRSLIRVLVPGANVLVASNPLL
                     SHLVSGALTRGGLERRGEIVRLVMTMRGGDRPKAAHVEFSDVSVMPRSMWEPWLVFRK
                     PLDGRVQDNLRRWGTGGFRRVSGDKPFGDVIESAPTRAAERQYAPHPSLKPQAFLRKV
                     VRGVLPLGNGMVLDPFAGSGSTLAAAEAVGYESVGVERDESYFELAKRALPELAKLKV
                     A"
     gene            5182..6387
                     /locus_tag="VGA59_01795"
     CDS             5182..6387
                     /locus_tag="VGA59_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9718437.1"
                     /translation="MTATERRASGSLATIFAARMLGLFLVLPVFALEASKYPGGDDPA
                     QVGLAMGIYGLTQGILQIPFGLASDRLGRKRVIIFGLLVFAAGSFLAAAADSLTWLII
                     ARSLQGAGAVSAAVTALLADQTRDQVRTKAMAIVGASIGLMFALSLVAAPLLAARIGL
                     AGLFVVTGVLAMACIAVVAWWTPAEPLQHKNIPRGKLSEVLNHAGLLRLDVGVFVLHA
                     VQLAMWVAVPALLVQAGLPKAQHWYIYLPAVLGSFVIMGALFPLERRGYLRAVFLGAV
                     AMVMLVQIGLLLAAGRPGIGVLGLLLFVFFCGFNVLEASQPSMVSRIAPSHARGGALG
                     VYNTLQSLGFFAGGAVGGWLTKNVGAQGLFAACAGLMLLWLAVAWPMRAPRAERSAEE
                     VLADEAQAT"
     gene            6465..6953
                     /gene="ssb"
                     /locus_tag="VGA59_01800"
     CDS             6465..6953
                     /gene="ssb"
                     /locus_tag="VGA59_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX9718438.1"
                     /translation="MASVNKVIIVGNLGRDPEMRTFPSGDRVANVTVATTDKWKDKQT
                     GELKEATEWHRVVFNGRLAEIAGEYLRKGSQVYVEGSLRTRKWTDKDGIEKYTTEIRA
                     DQMQMLGSRHGMGGPGADEEDPGGARRAAPASRPAAPAAQRPAASKPSTGFDDMDDDI
                     PF"
     gene            complement(7072..7479)
                     /locus_tag="VGA59_01805"
     CDS             complement(7072..7479)
                     /locus_tag="VGA59_01805"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEX9718439.1"
                     /translation="MTLLVWDAKYCVNIREIDRQHRKLFDLLNDLYEAMQEGLGKDLI
                     GEALGNLVDYTAYHFIWEEKLFRQHGYAEEAAHRAGHARLTTQLNAMKQKFDDGQTNV
                     TMDMLRYLRDWLNDHVTGEDRKYAPFLIGKGVS"
     gene            complement(7670..8776)
                     /gene="mnmA"
                     /locus_tag="VGA59_01810"
     CDS             complement(7670..8776)
                     /gene="mnmA"
                     /locus_tag="VGA59_01810"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX9718440.1"
                     /translation="MNRKPRVVVGLSGGVDSAVTAHLLKAQGWDVVGIFMKNWEDDDD
                     SEYCSSNEDFVDAAAVADVIGIEIEHVNFAGEYKDRVFAEFLREYQAGRTPNPDVLCN
                     AEIKFKAFLDHAMRLGAEKIATGHYARVRERGGMFELLKGLDETKDQSYFLHRLNQAQ
                     LGKTLFPVGELRKTEVRRIAQEIGLPNAKKKDSTGICFIGERPFREFLNRYISKEPGP
                     VKDERGRVIGRHQGLSFYTLGQRQGLGIGGIKEKGAQRGGGDHAPWYVARKDIDRNTL
                     WVVQGHDHPWLLSSVLTADDASWVSGRVPAPGAYAAKTRYRQADAACRLHSAANGAFQ
                     LDFSEPQWAVTPGQSAVLYNCEICLGGGVIAAAH"
     gene            8850..8954
                     /locus_tag="VGA59_01815"
     CDS             8850..8954
                     /locus_tag="VGA59_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9718441.1"
                     /translation="MSPDVLVVGGGNAALCAALMAREAGASVLPDWPY"
     gene            complement(8986..9465)
                     /locus_tag="VGA59_01820"
     CDS             complement(8986..9465)
                     /locus_tag="VGA59_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9718442.1"
                     /translation="MDTRWKPSVTVAAVVERDGRFLLVEEETAQGLMLNNPAGHLDAG
                     ESPADGCAREALEETAHGFRPTALLGIYLCRTRRESTGEDVTYLRLAFCGELGAHDPS
                     RALDRGIVRTLWLTPEEIRASVHRHRSPLVLRCVEDYLAGIRHPLSMVYTDPSVAGE"
     gene            complement(9518..10240)
                     /locus_tag="VGA59_01825"
     CDS             complement(9518..10240)
                     /locus_tag="VGA59_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718443.1"
                     /translation="MAHLASLSSPATGARASNVNYLGAARCRRKFELFYPDGFQDETY
                     QVAERSYKERAHLEWQTELGQGAFRKLLARGEFRRIADIAVRIESRTNLLFSFEKMAL
                     RDAVKTAAGARLFATELYAFLWGRGSPQRKFEDWVQAVADLPRRRTRVLTWPLVTVFG
                     FIARPEKYLFLKPNATRKAARSYGFDFEYRPQPSWSVYQCLLTFAAIIRRDLDRKPGF
                     KARDMIDVQSFIWVQGSQEYEP"
     gene            complement(10358..11503)
                     /locus_tag="VGA59_01830"
     CDS             complement(10358..11503)
                     /locus_tag="VGA59_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9718444.1"
                     /translation="MPLAFVLSLSAALHLYIGARIVPAIPGHFAAALVGLMLIASSVL
                     VPTGFFARRLAKPRVAATLTWAGFVFIGLFSSLLVLTVLRDALLLAGSILNVAIASAL
                     PIDALRADSAIAVPLLGIAATFLGYVNARRTAAVVNLEVPVPGLPAALHGFTIVQITD
                     IHVGPTIGYRYVEAIVEAVNRLRPDVVAITGDLVDGSVAELASQVAPLARLVSRHGVY
                     FVTGNHECYSGVDAWLAQFRRLGMTVLHNEHVVISHGGAKLVLAGVPDYSGGHFDSAH
                     RSDPQAALAGAPADAAVKILLAHQPRSAAAAERAGFDLQLSGHTHGGQFLPWTFLVRL
                     QQPFTAGLHRIGRLRVYVSRGTGYWGPPKRLGAPSEISEVRLTRPTP"
     gene            11772..13424
                     /locus_tag="VGA59_01835"
     CDS             11772..13424
                     /locus_tag="VGA59_01835"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX9718445.1"
                     /translation="MLIDVPAETTAGETRVAVTPETAKKSLRIGVPKETAAGEKRVAT
                     VPEVVEKLVKLGFSVAVESGAGDAANCGDDAYRTAGAEIAATAAELWAKSDIVFKVRA
                     PSAAEVGLMHEGQTLISFIWPAQNPELMQQLAARKVTVLAIDSLPRTLSRAQKMDALT
                     SQAGVAGYRAVVEAANAFGRFFNGQITAAGKVQPAKVFIAGAGVAGLAAIGTAASLGA
                     IVRANDTRAEVADQVVSLGGEFVKVDYEEEGSGGGGYAKVMSEGFQKAQREMYAKQAK
                     EVDIIITTALIPGKPAPRLITAEMVRSMKPGSVIVDMAAEQGGNCELTEPGQAVVKHG
                     VTIVGYTDLVSRLAKQSSTLYATNLFRLSEELCKTKDGIIDVNMEDEAIRGLTVIKQG
                     NITWPPPAPKVAAAPAAKPAAMPAKAPKHGEASAPASATRTAVMFGIAALAFWFVGAN
                     APAAFLGHFTVFVLACFVGYMVVWNVTPALHTPLMSVTNAISSIIAIGALVQIAPPLV
                     GGGAARPEQWIHWLAWVGIALTAINMFGGFAVTQRMLQMFRK"
     gene            13437..14852
                     /gene="pntB"
                     /locus_tag="VGA59_01840"
     CDS             13437..14852
                     /gene="pntB"
                     /locus_tag="VGA59_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEX9718446.1"
                     /translation="MGATLATASYIGATILFILSLGGLSNPESSRRGNLYGMIGMTIA
                     VLATVSGPQVTSAGYAWIIGAMLIGGSIGLYASRIVRMTQMPELVALMHSLVGLAAML
                     VGFANYIDPAASAEFTGAAKSIHEMEIYIGVLIGAVTFTGSVIAFGKLSGKIGGKTLL
                     LPGRHWLNLAGLLVVIWFGREFINAHSIADGMIPLIVMTVIALLFGIHMVMAIGGADM
                     PVVISMLNSYSGWAAAATGFMLSNDLLIVVGALVGSSGAILSYIMCRAMNRNFISVIA
                     GGFGSEGGAPAPAGGVQPAGEVNPVLAAETAELLRESKNVIIVPGYGMAVAQAQHTVY
                     EITKVLREKGVNVRFGIHPVAGRMPGHMNVLLAEAKVPYDIVLEMDELNEDFPNTDVA
                     MIIGANDIVNPAAQEDPSSPIAGMPVLEVWKAKTSIVMKRSMATGYAGVDNPLFYKDN
                     NRMLFGDAKKMLDEVLAALKA"
     gene            15134..16810
                     /locus_tag="VGA59_01845"
     CDS             15134..16810
                     /locus_tag="VGA59_01845"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX9718447.1"
                     /translation="MSDRPWLKSYSPGVPADIDPSQYESLVQLMEESFKQYASRPAYS
                     FMGKEVTFGQTDSLSRALAAYLQSLGLARGDRVAIMMPNVPQYPVAVAAILRAGFVVV
                     NVNPLYTPRELEHQLKDSGSKAIVIIENFASTLEQCIANTPVKHVILCSMGDQLGLLK
                     GALVNYVVRNVKKMVPAFNLPGAVRFNEAIALGTRGSFKKPEIKADDVAVLQYTGGTT
                     GVSKGAVLLHRNIIANVLQSQVWNQPVMDRVPANEQSTSVCALPLYHIFAFTVGMMLS
                     MRTGGKLILIPNPRDLPAVLKELSKHTFHSFPAVNTLFNGLANHPDFDKVNWKNLKVS
                     VGGGMAVQGAVAKLWLEKTGCPICEGYGLSETSPSASCNPVTSKEYTGTIGVPLSSTY
                     MKLLDDEGHEVAPGQPGEIAIKGPQVMAGYWQRPDETAKVMTPDGFFKSGDIGVVDER
                     GYFKIVDRKKDMVLVSGFNVYPNEVEEVVAGLDGVLECAVVGVADDKTGEAVKLVIVK
                     KNPDLTEAQVREFCKANLTGYKQPKVIEFRTDLPKTPVGKILRRELRDKK"
     gene            complement(16917..17084)
                     /locus_tag="VGA59_01850"
     CDS             complement(16917..17084)
                     /locus_tag="VGA59_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718448.1"
                     /translation="MRLTLIESYRPARKLSKIKKAVHRFGPAAALQRKSAAFGRAYLS
                     QLVEKRVAFLE"
     gene            17120..17833
                     /locus_tag="VGA59_01855"
     CDS             17120..17833
                     /locus_tag="VGA59_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718449.1"
                     /translation="MTNMTSAAAANLTGAASQTRPWALAAGLCWATSALAAGGHHALD
                     DAVILEPGNCQVETWLSRSDDRQRLLHVGSGCRVGPVEVGVAAEHARQSGASDTGYGL
                     QAKWATELLPGFNAGVSLSTGWQAHVRPRYQGSTVSGLFSWFPRDDLAFHLNLGRDFV
                     HRDEDQDRSGVSVEWTARPGWSITAERYLEAQTHFVRAGVRWAISEAWSVDLSRAHRL
                     RGPGESNWTLGATWQFPRP"
     gene            complement(17834..18883)
                     /gene="mtnA"
                     /locus_tag="VGA59_01860"
     CDS             complement(17834..18883)
                     /gene="mtnA"
                     /locus_tag="VGA59_01860"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX9718450.1"
                     /translation="MSSSQGNGVQTLRWCNGQLEMIDQRVLPAEFRYVRYDSAEAVAH
                     GIRDMVVRGAPAIGCAAAYGIALEALRQQDAPASDFTVLMERAFAVLAASRPTAVNLF
                     WALRRMRMRWQSIAAQPAASQAQALLDEAHEIFAEDIRVNRAMGEHGAALLADGARVL
                     THCNAGALATAGHGTALGVIRSAVEAGKRISVVAGETRPFLQGARLTAWEMVQEGIPV
                     TLITDNMSGFLMSRGEIDAVIVGTDRVAANGDVANKIGTYMVAVLAHRHRIPFYVACP
                     TSTIDMSIAEGAAIPIEERASEEVTGFRDYRWAADGVSVRNPAFDVTPAELVTALITE
                     RGVVHQPNRERLARL"
     gene            complement(18880..19554)
                     /locus_tag="VGA59_01865"
     CDS             complement(18880..19554)
                     /locus_tag="VGA59_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX9718451.1"
                     /translation="MQDEMEGEAKLRRAMVQAALELAGRGLNRGTSGNVGVRCGDRIL
                     VTPSGVPARELTPQGMVLLAANGAVIGAGRPSSEWRFHRDILEWRPDVNAIVHVHSPF
                     ATSLACLGLEIPPFHYMIAAAGGTTIRCAPYALFGTQELSDRALAALQDRRACLLANH
                     GMIATGRDLDHAVALCIEVESLCEQYWRARQIAEPCRLSDAQMAEVMEKFKTYGQHAQ
                     GEQAEP"
     gene            complement(19544..20446)
                     /locus_tag="VGA59_01870"
     CDS             complement(19544..20446)
                     /locus_tag="VGA59_01870"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX9718452.1"
                     /translation="MNSRIAVAPVIGVLGGSGVYDIDGLSNKRWAKVASPFGEPSDEL
                     LYGDLNGQPMVFLPRHGRGHRIAPSEINFRANIDALKRSGVTDLISVSAVGSLREELP
                     PGTFVIVDQFIDRTFARNKSFFGTGLVAHVSLAHPVCRRLGDHLQAAAREAGIPVVRG
                     GTYLVMEGPQFSSLAESELYRTWGCHVIGMTNMPEAKLAREAEMCYATVAMVTDFDCW
                     HPDHDAVTVNQIIQVLLANAEKARSLVRHVAPHIAADGGAPACVCRHALEHALITAPD
                     VRDPALVARLDAVAGRVLGVKNAG"
     gene            complement(20588..>20803)
                     /locus_tag="VGA59_01875"
     CDS             complement(20588..>20803)
                     /locus_tag="VGA59_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX9718453.1"
                     /translation="FININQNKIIKIFSVAAVALLPPTLIASIYGMNFKFMPELDWQA
                     GYPFALALMLASALGPMWYFRRRGWLK"
BASE COUNT         3513 a         6788 c         6854 g         3648 t
ORIGIN      
        1 cgcttggcgc aggtagcggc cggtgtggct gtccgggtgc tgcgccacct gctggggcgt
       61 accctgggcg acgatgcgcc cgccgccgtc gccgccctcg gggccgaggt ccacgatcca
      121 gtcggcggtc ttgatcacgt ccaggttgtg ctcgatcacc acgatggtgt tgccggcgtc
      181 gcgcagctgg tgcagcacgc gcagcagcag cgcgatatcg gcgaagtgca gcccggtggt
      241 cggctcgtcc aggatgtaga gcgtgcggcc ggtgtcgcgc ttggacagct ccagtgccag
      301 cttcacacgt tgcgcctcgc cgcctgagag cgtcgtcgcg gcctgcccga gcttgatgta
      361 cgacaggccc acgtccaata gggtttgcag cttgcgggcg atggtaggaa ccggcttcaa
      421 gaacaggtgc gcgtcctcca ccgtcatgtc caggatctgc gcgatgttgc gccccttcca
      481 ctgcacctcc agcgtttcgc ggttgtagcg ctggccatgg cagacgtcgc atgccacata
      541 gacgtcgggc aggaagtgca tctccacctt gacgacgccg tcgccctggc aggcctcgca
      601 gcgcccgccc gccacgttga acgagaaacg ccctgggcca taaccgcgct ccttggccat
      661 ggacacctcg gccatcagct cccggatcgg cgtgaagacc cccgtgtagg tggccgggtt
      721 gctgcgtggc gtgcggccga tcggcgactg gtccacgttg atcaccttgt cgaagtgctc
      781 gatgccttcc acggcttcat gcggcgccgg ctcctcgtgg gcacggtaaa gcgtgcgcgc
      841 caccgccgcg tagagcgtgt cgttgaccag agtcgatttg cccgagcccg agacgccggt
      901 gacgcaggtg aacaggccga ccggaaacgc ggcgttgatg ttcttgaggt tattcccgct
      961 ggcgccaagg acgcgtatct cctggaccac gccgcgggtc gcgagatgtt cggcctcgcg
     1021 tgccgcgcgg cgcagggctg cgtcgctggg cggaaaacgc gaggatttcc tcatctcagc
     1081 cgggatcagg tccagcaccg gtaaccagcg cgtgcggcgc gcgggtaccg ggatgctctt
     1141 cttgccggag aggtactggc ccgtcaggga cgcggccgtc gccttgatgt cctcgtagct
     1201 gccttgcgcc atgacgcggc cgccgtggat gccggcgccc aggcccatgt cgatcaggtg
     1261 gtcggcagcc cggatcatgt cttcgtcgtg ctccaccacg atcacgctgt tgccgatgtc
     1321 gcgcaggtgc cgcaaggtgc cgatcaggcg gtcgttgtcg cgctggtgca ggccgatgct
     1381 cggttcgtcc agcacgtaca tcacgccggt caggcccgat ccgatctgcg aggccaggcg
     1441 gatgcgctgc gcctcgccgc ccgacagtgt ctcggcgctg cggtccaggc tcaggtagtt
     1501 cagtcccacg tcgttgagga atttcagcct gaggccgatc tcgcggatca ccttctccgc
     1561 gatctcggcc ttggcgccgc gcagtttgag gccatcgaaa tactcgaagc tctcgcgcaa
     1621 ggtggcgtgg ccgatctcgt agatggcgcg ggcctcgccg ccttcgccga tcttgacgtg
     1681 cctggcctcg ctgcgcaggc gcgtgccgcg gcagtcgggg cagggctgca ggctgcggta
     1741 gcgagccaga tcctcgcgca ccatgaccga atcggtttcc cggtagcggc gctccctgtt
     1801 gggaatgacg ccctcgaagg tgtgcttctt ggtgatcctt cgccccgcgg aacttcttcc
     1861 cccccacgct tcgctgcccc ccgagggggc gttcgccgtc ttggggcggc ccggcgacgt
     1921 cgagcccgag tccagcgtgt agctgaaccg gatttcttca tcgcccgagc catgcaggat
     1981 cgcttgttgc accggcgggg ccagttcctc gaacgggacc tcgaggctga acttgtagtg
     2041 ccgcgccagg ctctcgagca gcgcgaagta ataggcgttg cgccggtccc agcccttgat
     2101 ggcgccgctc gccagcgaca ggctgggaaa ggcgacgact cgcgccgggt cgaagaattc
     2161 cctgtggccc aggccgtcgc aggtcgggca ggcgcccacc ggcgagttga acgagaacag
     2221 gcgcggctcc agttcggtga tcgaatggtt gcagatcggg caggcgaact tcgcgttgaa
     2281 ccagtgctcc cggccgctgt ccatctccac cgcgatggca cggccgtcgg ccagccgcag
     2341 cgccgcctcg aaactctcgg ccaggcgctg ctgcgagcct gccccgggcc ccgacccggg
     2401 gtcggggcgc accttgagcc ggtcgatcac cacgtcgatg tcgtgcttct cggccttctt
     2461 cagcctgggc agcgcgtcga attcataagt ctggccgtcc acccggaagc gcacgtagcc
     2521 gcgcgcctgc atctcggcga acacgtccag gaactccccc ttgcggtcgc gcgccaccgg
     2581 cgccagcacc atgagcctgc tttcggccgg caatgcgagc accgcatcca ccatctgcga
     2641 cacggtttgc gattgcagcg ggatgtcatg gttcgggcag taaggcgtgc cagcccgcgc
     2701 gaacagcagc cgcaggtagt catggatttc agtgaccgtg ccgaccgtgg agcgcgggtt
     2761 gtgagaggta gccttctgct cgatcgagat cgccggcgac aggccttcga tcacgtccac
     2821 gtcgggcttg tccatcagct gcaggaactg ccgtgcatag gccgagaggc tttccacata
     2881 gcgccgctgg ccttcggcgt acaaggtgtc gaaggcgagc gaggacttgc ccgaacccga
     2941 caggccggtg atcaccacca gctggttgcg cgggatatcc aggtcgatgt tcttgaggtt
     3001 gtgggtgcgc gccccgcgga tgctgatccg ctgcgccatt gcggcggccg ggtacttgcc
     3061 gctgtcgcgc gacttggcgc tttcgagggg ctcgagggac aggccttcgg tcgaggagtt
     3121 caatgtcggg tgcgccaagg gaaaccggac atgatagccc ggcggcgatt tttccgaccg
     3181 cccggcacag tcctcgcttc actgccgacc cggcgcggct cggaggatcg caatggacga
     3241 gcagaaagct tgtcggtcct attttttcgc gtccgggaat aaacggggat ggcgggtgtt
     3301 catgcggatg tcggtcacga cccgccgcaa cgcttccagc ccgtccattc acaggagacc
     3361 ccgatgaaaa ataccctgtc agcttgcctg ctgtctctcg cctcgttggc gccgctcacc
     3421 gcggccgcga tagaaccgtt ggtccgcttc gaggacggca tcggcgtgca gccgctgcgg
     3481 tcgggcccgg cgccgaacgt ggtgttcggc gtgaatccgg gcggcctgcc gtgggtgatc
     3541 tcccggctga gcgccgacgt gagcaccgac ggcaggatcc gggtggacgg ccgtggcctg
     3601 ctgttcgcgg ggggcgacaa catcggcagg accggcggac aaagcgtgcg cgcgcggctg
     3661 atctgcgccg gcgtgtacca cgactcggac ctggtgccgc tcgacgcccg cggcgatttc
     3721 acgatcggcg gcttcctgaa tccctttccg ccttctccct gcgcgacgcc ggtcctgctg
     3781 atcgtcaacg ccggcggcaa ctggttcgcc gccggtattc ccaagccgta ggccaaggca
     3841 cctccctgcc ccgggcacgt ggggcgtgcg tgcgggcgct gccgaagtga gcgcgaatcg
     3901 agcgtttgcg cgcatgcagg acaatctggg ttttcccggg ccactgccgc ccgctaccaa
     3961 aataaatata gcttggccct tctcaaaaga cgtctagacg tcctcaacac tcccacactg
     4021 ttcgcacgta cggaccgtgc gcaatacctg ctgagcggat ttgacatggg cccggcagtg
     4081 gcaggtccga tgcaagcccc ggtgcctcgt ttcgattttg gtcgggcttc tctttttcgt
     4141 gaggattgcc ttgcgtggct cgacaagcga cccgccaatt caatccacgc ggttgtgacc
     4201 gatcctcctt atggactcct ggagtattcc gatgcggaac aggtaaagct gagatcaggc
     4261 cgcggcgggg tgtggcgaat tccgcccagc ttcgatgggt ccaggagagc acccttgccc
     4321 cgatttaccg ttctcaagcc caatgatctc caagttctag agcaattctt cctcgcttgg
     4381 actcgtagct taattcgggt gcttgtgcct ggagccaatg tgctggtcgc gagcaatccc
     4441 ttactttccc atctcgtgtc cggtgcgctg actcggggcg ggcttgagcg cagaggggaa
     4501 attgttcgcc ttgtcatgac gatgcgcggc ggtgatcgcc cgaaggctgc ccacgttgag
     4561 ttcagcgatg tgagcgtgat gccccgatcg atgtgggaac cctggctggt gtttcgcaag
     4621 ccgctcgatg gtcgggtcca ggataatttg cgcaggtggg gaacaggagg attccgccgc
     4681 gtttcaggtg acaaaccgtt tggagacgtt attgagtcgg cccccactcg cgcagccgag
     4741 cgccagtacg ctcctcatcc aagcctgaag ccgcaagcct tcttgcgcaa agtcgtccgc
     4801 ggagtgttgc ctttgggcaa tggaatggtg ctggatccct tcgccgggtc gggctcaact
     4861 cttgccgccg ccgaggcagt gggttacgaa agcgtggggg tggagcggga tgagtcctac
     4921 tttgagcttg ccaagcgcgc cctacccgaa cttgcaaaac tgaaggttgc ctgaccgccg
     4981 tgcattgtca aaggcggtaa atccagttct gccgcagttt tgcaattcct tgacatggcc
     5041 ggctcacttc ttccgccttt cggggcgctt ctgagcgaat gccggacaat cccaagtttg
     5101 ccggggtcgc ctttcgggcg gcccggttcc tgctcgcgaa cgcccgtgaa tacaacccct
     5161 atttccatcg actccggccg catgacggcc accgagcgcc gcgccagcgg ctcgctcgcc
     5221 accatctttg ccgcacggat gctgggcctg ttcctggtgc tgccggtgtt cgcgctggag
     5281 gcgtccaaat atcccggcgg cgacgacccg gcgcaggtcg ggctggcgat gggcatctac
     5341 ggcctgaccc agggcatcct gcaaatcccg ttcggcctgg cttcggaccg gctgggccgc
     5401 aagcgcgtga tcatcttcgg cctgctggtg ttcgcggccg gcagtttcct cgcggccgcg
     5461 gccgacagcc tgacctggct catcatcgcg cgctcgctgc aaggcgcggg tgcggtgtcg
     5521 gcggcggtga cggcgctgct ggcggaccag acccgcgacc aggtgcgcac caaggccatg
     5581 gccattgtcg gcgccagcat cgggttgatg ttcgcgctgt cactggtggc cgcaccgctg
     5641 ctggcggcgc ggattggcct ggccggcctg ttcgtggtca ccggggtgct ggccatggcc
     5701 tgcatcgccg tcgtcgcctg gtggacgccg gccgagccgt tgcagcacaa gaacattccg
     5761 cgtggcaagc tgtccgaagt gctgaaccat gccggcctgc tgcggctgga cgtgggcgtg
     5821 ttcgtgctgc acgcggtcca gctggcgatg tgggtcgcag tccccgccct gctggtccag
     5881 gcggggctgc ccaaggcgca gcactggtat atataccttc cggcggtgct ggggtccttc
     5941 gtcatcatgg gggccctgtt cccgctggag cggcgcggct acctgcgtgc cgtgtttctc
     6001 ggcgcggtgg cgatggtgat gctggtgcag atcgggctgc tgctggcggc gggccggccg
     6061 ggcatcggcg tgctgggcct gctgctgttc gtattcttct gcggcttcaa tgtgctggag
     6121 gcgtcccagc ccagcatggt gtcgcggatc gccccctctc atgcccgcgg cggggccctg
     6181 ggcgtctaca acacgctgca atccctgggt ttcttcgcgg gcggggcggt cggcggatgg
     6241 ttgaccaaga acgttggagc acaaggcctg tttgccgcct gcgccgggtt gatgttgctc
     6301 tggctggcgg tggcctggcc tatgagggcg cccagggcgg aaagatcggc cgaggaagtg
     6361 ctggccgatg aggcccaggc gacataattg gcgtttatcg aggccggcgg ctgacgccgc
     6421 ggctcatcgt cggtcacaac ccaccaagtg gaggagcaaa ttcaatggca tccgtcaaca
     6481 aagtcatcat cgtgggcaat ctcgggcgcg acccggaaat gcgcaccttt cccagcgggg
     6541 accgcgtcgc caacgtcacg gttgccacca ccgacaagtg gaaggacaag cagaccggcg
     6601 agttgaagga agcgaccgaa tggcatcgtg tcgtgttcaa tggccggctg gccgagatcg
     6661 caggggagta cctgcgaaag ggctcccagg tctacgtcga aggcagcttg cgcacgcgca
     6721 agtggaccga caaggacggc atcgagaagt acacaaccga aatccgcgcc gaccagatgc
     6781 agatgctggg cagccgccac ggcatgggcg gcccgggggc cgatgaagaa gacccgggtg
     6841 gcgcccgccg cgccgcgccc gcgtcgcgcc cggccgcacc ggcggcgcag cgccccgccg
     6901 cgtccaagcc gtcgaccggc ttcgacgaca tggacgacga tattccgttc taagccgaaa
     6961 aagccgctca ttggttgatt gcggcccgct ttctcctgcc cgaaagcggg atgcatcgcg
     7021 gatggcgccg gcgcagcccc gggcgcgtgc gtggcggcgg ctcgtcgcgc cttaactcac
     7081 gcctttgccg atgaggaacg gcgcgtactt gcggtcttcg cccgtgacgt gatcgttgag
     7141 ccagtcacgc agatacctga gcatgtccat ggtcacgttc gtctggccgt cgtcgaattt
     7201 ctgcttcatc gcgttcaact gcgtggtgag cctggcgtgc cctgcgcggt gagcggcctc
     7261 ctcggcgtag ccatgctggc ggaaaagctt ctcctcccag atgaagtggt acgcggtgta
     7321 gtcgaccagg ttcccaagcg cctcgccgat gaggtccttg ccgagccctt cctgcatggc
     7381 ttcgtacagg tcgttcagca agtcgaacag cttccggtgc tgccggtcga tctcacggat
     7441 gttcacgcag tacttcgcgt cccaaaccag aagtgtcatc ggttggttcc cttcagagga
     7501 gcaacataac ggctcgagcc agggccggac ttgatccagc gcaacacgcg cttggagcct
     7561 gtggctttca aagccgcgat caaaggagtg tttgcggccc gattcgaaag gaccgcattg
     7621 catcgatcat ggcctgggcg gccgacctgc cggcccgagc cgctgccggt cagtgcgctg
     7681 ccgcgatgac gcctccgccc aggcagatct cgcagttgta gagtacggcc gactgtcccg
     7741 gcgtgaccgc ccattggggc tcgctgaagt cgagctggaa cgcgccgttg gccgcgctgt
     7801 gcagcctgca ggccgcgtct gcctgccggt accgggtctt ggcggcatag gcgccggggg
     7861 ccggcacccg gccggaaacc cagctcgcgt cgtccgccgt cagcaccgat gacagcagcc
     7921 aggggtggtc atggccttgc actacccaga gggtgttcct gtcaatgtcc ttgcgcgcca
     7981 cgtaccaggg cgcgtggtcg ccgccgccgc gctgcgcgcc cttctccttg atgccgccta
     8041 tgcccaggcc ctggcgctgg ccgagggtgt agaaggaaag gccctggtgc cggccgatca
     8101 cccggccgcg ctcatccttc acgggcccgg gttccttcga gatgtagcgg ttgaggaatt
     8161 cgcggaaggg ccgctcgccg atgaagcaga tgccggtgga atccttcttc ttcgcgttgg
     8221 gcagtccgat ctcctgggcg atgcgccgca cttccgtctt gcgcagctcg cccacgggga
     8281 acagcgtctt gcccagctgg gcctggttca ggcggtgcag gaaatagctc tggtccttgg
     8341 tctcgtccag gcctttcagg agctcgaaca tgccgccccg ttcccgcacc ctggcgtagt
     8401 ggccggtcgc gatcttttcc gccccgagcc gcatcgcgtg atccaggaat gccttgaact
     8461 tgatctcggc attgcacaga acgtcggggt tgggcgtgcg cccggcctgg tattcgcgca
     8521 ggaactcggc gaaaacgcgg tccttgtatt cgcccgcgaa attcacatgc tcgatctcga
     8581 tcccgatcac gtcggccacg gcggcggcgt cgacgaagtc ctcgttggac gagcagtatt
     8641 cgctgtcgtc gtcatcttcc cagttcttca tgaagatgcc gacgacatcc cagccctggg
     8701 ctttcagcag gtgcgcggtc accgcggagt ccacgccccc gctcaacccc acgacgaccc
     8761 tcggcttgcg attcatttgt gcgattattc cagggcgccc acatccttat ggtcacaggc
     8821 ccacttcccc gtcgccgccg cgccggcgca tgtcaccgga tgtcctggtg gtcggcggcg
     8881 gcaatgccgc gctgtgcgcc gcgttgatgg cgcgggaggc cggtgccagc gtcctgccgg
     8941 actggcctta ttaatttggc gccgctgccg ggcgttcgca gagagtcatt ccccggcgac
     9001 gctggggtcg gtgtagacca tcgacagcgg gtgccggatg ccggccaggt agtcctccac
     9061 gcagcgcaac accagcgggc tgcgatggcg gtggacgctg gcgcggatct cctcgggcgt
     9121 gagccacaag gtcctgacga tgccccggtc gagcgcacgg gacgggtcgt gcgcgcccag
     9181 ttctccgcaa aacgccagcc tcaggtaggt gacgtcctcg ccggtcgatt cgcgccgcgt
     9241 ccggcacaga tagatgccga gcagcgcggt gggacggaag ccatgcgccg tttcctccag
     9301 cgcctcgcgc gcgcagccgt cggccggcga ctcgcccgca tcgaggtggc cggccggatt
     9361 gttcagcatc aggccttggg cggtctcttc ctcgaccagc aggaaacggc cgtcgcgttc
     9421 gacgaccgcc gccaccgtga cgctgggttt ccatcgggta tccatcgcat cgattatggc
     9481 cggggccctc gcatcggtgc accgccctgc cgctgcctca aggctcgtac tcttgcgagc
     9541 cctggaccca gatgaaggat tgcacgtcga tcatgtcgcg ggccttgaag cccggcttgc
     9601 ggtccaggtc gcggcggatg atggccgcga aggtcagcag gcactggtag acgctccacg
     9661 acggctgcgg ccggtactcg aaatcgaaac cgtagctgcg cgccgccttg cgcgttgcgt
     9721 tgggcttcag gaacaggtat ttctccggcc gcgcgatgaa gccgaacacc gtcaccagcg
     9781 gccaggtcag cacgcgcgtc cggcgccggg gcaggtcggc cacggcctgc acccaatcct
     9841 cgaatttgcg ttgtggcgac cctcgacccc agagaaacgc gtacagctcc gtggcgaaca
     9901 gccgcgcacc ggcggcagtc ttcaccgcgt cgcgcagcgc catcttctcg aacgagaaca
     9961 gcaggttggt gcgcgactcg atgcgcacgg cgatgtcggc gatccggcgg aactcgcccc
    10021 gggccagtag cttgcgaaag gcgccctgcc cgagttcggt ctgccactcg aggtgggccc
    10081 gttccttgta ggaccgctcg gcgacctggt aggtctcgtc ctggaaaccg tccggataga
    10141 acagctcgaa tttacggcgg cagcgagccg ctccgaggta gttgacgttg ctggcgcgcg
    10201 cgcccgtggc gggggaagaa agcgaagcta ggtgtgccat gcaggcagtt tttggcgtgc
    10261 cccaacctca gccgtgtcgg acgacaccct gcgtccatgt aggaatcgga tggcgcagtg
    10321 catttctccc atccggtcga gtcgcccatg ccccaaatta gggcgtggga cgggtcaacc
    10381 gcacttcact gatctcggac ggggcgccca gacgtttggg gggaccccag taaccggtgc
    10441 cacggctgac atagacccgt aatcgcccga tgcgatgtag cccggccgtg aacggctgct
    10501 gcaatctcac caggaaggtc cacggcagga actggccacc gtgggtatgg cccgacagct
    10561 gcagatcgaa cccggcccgc tcggccgccg cagcgctgcg aggctggtgg gccagcagga
    10621 tcttaacggc cgcgtcggcc ggcgcgcccg ccaatgccgc ctgcgggtcg ctgcggtggg
    10681 cgctgtcgaa gtggccgccg ctgtagtcgg gcaccccggc cagcaccagc ttggcgccgc
    10741 catgcgagat cacgacatgc tcgttatgca gcaccgtcat gcccaggcgc cggaattgcg
    10801 ccagccaggc gtcgacgccg gagtagcact cgtggttgcc ggtgacgaaa tagacgccgt
    10861 gcctggaaac cagccgggcg agcggcgcga cctggcttgc gagctcggcc acggagccgt
    10921 ccaccaggtc gcccgtgatc gccacgacat cgggcctgag gcgattcacg gcctcgacga
    10981 tggcttcgac atagcggtag ccgatggtgg gcccgacgtg gatatcagtg atctggacga
    11041 tggtgaagcc gtgcagggcc gccggcaggc cgggcacagg tacttccagg ttcaccactg
    11101 cggcagtgcg ccgggcattc acgtacccga gaaaagtcgc cgcaatgccg agcagaggca
    11161 cggcgatggc gctgtcggcg cgcagggcat cgatgggcag cgcagatgct atagcgacat
    11221 tcaagatcga tcccgcaagc agcaaagcgt cgcgtagcac cgtgagcacc agcaacgacg
    11281 agaacagccc aatgaacacg aaccctgccc aggtcaatgt cgcggcaacg cggggtttcg
    11341 cgaggcgccg cgcgaaaaaa ccggtcggaa cgagcaccga cgaggcaatc agcatgagcc
    11401 caaccagcgc ggcggcgaaa tgaccgggga tggccggaac gatgcgcgcg ccgatgtaca
    11461 ggtgcagcgc ggccgacaaa ctgagcacaa aggccagggg catgggtgcc aactggtgcc
    11521 ggattcgcca attgaaagcg gctagggcct gaaagcggtt agagccgctc cttggcgcgg
    11581 tcgggagacc ggctatcgga attttctcat ttgcgggctg aaactgcgat gaatcaaacg
    11641 aaacccgcgc actttcgatt aacctccgcg cgccagatga tagaaggtct tttcacattg
    11701 cggaacgcgg atttcgttta agctcgcggc cgcaagtccc acaaccgttt taccaacgag
    11761 gagagtcgtc catgctgata gacgttcccg ccgaaaccac ggcgggtgaa acccgtgttg
    11821 ccgtcacacc cgagacggcc aagaaatctt tgcgtatcgg agttccgaag gaaacggcgg
    11881 cgggcgagaa gcgcgtcgcg accgtacccg aggtggtgga aaaactcgtc aagctggggt
    11941 tctcggtagc cgtggaaagc ggcgcgggcg acgctgcgaa ctgcggcgac gacgcctacc
    12001 gcactgccgg cgccgagatc gcggcaacgg ctgccgaact ctgggcaaag tcggacatcg
    12061 tcttcaaggt acgcgctccg tccgctgcag aagtcggcct gatgcacgag ggccagaccc
    12121 tgatcagctt catctggccg gcgcagaacc ctgaactgat gcagcagctc gcggcgcgca
    12181 aggtcaccgt gctggccatc gactcgctgc cgcggacgct ctcgcgcgcc cagaagatgg
    12241 acgctttgac ctcccaggcc ggcgtcgccg gctaccgcgc ggtcgtcgag gccgccaacg
    12301 ccttcggccg cttcttcaac ggccagatca cggccgcggg caaggttcag ccagccaagg
    12361 ttttcatcgc cggtgccggc gtcgccggcc tggcggcgat cggcacggct gccagcctcg
    12421 gcgccatcgt gcgcgccaac gacacccgtg ccgaagtggc cgaccaggtc gtgtccctgg
    12481 gcggtgaatt cgtcaaggtc gattacgagg aagaagggtc cggcggcggc ggctacgcca
    12541 aggtgatgag cgagggcttc cagaaggccc agcgcgagat gtacgccaag caggccaagg
    12601 aggtggacat tatcatcacc accgccttga tccccggcaa gcccgctccc cggctgatca
    12661 ctgcggagat ggtgcggtcg atgaagccgg gcagcgtgat cgtcgacatg gccgcggaac
    12721 aaggcggcaa ctgcgagttg accgagcccg gccaggcggt ggtgaagcac ggcgtgacca
    12781 tcgtcggcta caccgacctg gtcagccgtc tggccaagca gtcgagcacg ctctatgcga
    12841 cgaacctgtt ccgcctgtcc gaggagctgt gcaagaccaa ggacgggatc atcgacgtca
    12901 acatggaaga cgaagccatc cgcggtctca cggtgatcaa gcaaggcaac atcacctggc
    12961 cgccgcccgc gcccaaagtc gcggccgcgc ccgcggccaa gccggcggcg atgccggcca
    13021 aggcgcccaa gcatggcgag gcgagcgcac cggcttcagc cactcgcacc gcggtcatgt
    13081 tcggcatcgc cgcgctggcc ttctggttcg tcggcgcgaa cgcacccgcg gctttcctcg
    13141 gccacttcac ggtgttcgtg ctggcctgct tcgtcggcta catggtggtg tggaacgtca
    13201 cccccgccct gcatacgccg ctaatgagcg tcaccaatgc gatcagcagc atcatcgcca
    13261 tcggcgccct ggtgcagatc gcgccgccgc tggtgggcgg tggcgcggcg cggccggagc
    13321 agtggatcca ctggctggcg tgggtgggca tcgcgcttac cgcaatcaac atgttcggcg
    13381 gcttcgccgt cacgcagcgc atgctgcaga tgttccgcaa atagggaaag cgaatcatgg
    13441 gcgcaacgct tgcaactgcc tcctatatcg gggcgacaat actcttcatc ctcagcctcg
    13501 gcggcctctc gaatccggag agctccaggc gcggcaatct ctacggcatg atcggcatga
    13561 ccatcgccgt gctcgcgacg gtctccgggc cgcaggtcac ctcggccggc tatgcctgga
    13621 tcatcggcgc gatgttgatc ggcggcagca tcggcctgta cgcctcgcgc atcgtgagga
    13681 tgacgcagat gccggaactg gtggcgctga tgcacagcct ggtcggcctc gccgcgatgc
    13741 tggtcggctt tgccaattac atcgatccgg cggcctcggc ggaattcacc ggcgcggcga
    13801 agtcgattca cgaaatggaa atctacatcg gcgtcctgat cggtgcggtg accttcaccg
    13861 gttcggtgat cgccttcggc aagctgtccg ggaagatcgg cggcaagacc ttgctgctgc
    13921 cggggcgcca ctggttgaac ctcgccggcc tgctcgtggt gatctggttc gggcgcgaat
    13981 tcatcaacgc tcactcgatc gccgacggca tgataccgct gatcgtgatg accgtgatcg
    14041 cgctgctgtt cggcatccac atggtgatgg cgatcggcgg cgccgacatg ccggtggtta
    14101 tttcgatgct caacagctac tccggctggg cggcggcggc gaccggcttc atgctctcga
    14161 acgacctctt gatcgtcgtc ggcgcgctgg tcggttccag cggcgcgatt ctgtcttaca
    14221 tcatgtgccg ggcgatgaac cgcaacttca tcagcgtgat cgcgggtgga ttcggctccg
    14281 agggtggtgc gccggcgcct gcgggcggcg tacagccggc cggcgaggtc aatccggtcc
    14341 ttgccgcgga aacagcggaa ctgctgcgcg aatccaagaa cgtgatcatc gtgccgggct
    14401 acggcatggc ggtagcgcag gcgcagcaca ctgtctatga gatcaccaaa gtgctgcgcg
    14461 aaaagggtgt caacgtgcgc ttcggcatcc acccggtggc ggggcgcatg cccggtcaca
    14521 tgaacgtgct gctggcagag gccaaggtgc cttacgacat cgttctggaa atggacgagt
    14581 tgaacgaaga ctttcccaat accgacgtcg ccatgatcat cggcgccaac gatatcgtga
    14641 acccagctgc ccaggaagac ccgtcgagcc cgatcgccgg catgccggtg ctcgaagtgt
    14701 ggaaggccaa gacctcgatc gtgatgaagc gcagcatggc caccggctat gcgggcgtgg
    14761 acaatccgct gttctacaag gacaacaacc gcatgctttt cggcgacgcc aagaagatgc
    14821 tggacgaagt gctggccgcg ctcaaggcgt gagattgggg cccgcagcag aaggattccc
    14881 aagcgctgag attgtcgaag agatgggcaa ggcgatcgaa taacgcgcct gtcgcgcaag
    14941 cgactcccag gaacgcggcc cacgggccgc gttttgcttt tgcgccaccg catgttcgcc
    15001 ttggtttaga gggcctactc tcgaaaaccc tagggaaaac acaatgccgg gccgcatgag
    15061 ggcgcgacac aatccgccag cctgacgaaa accgcatgga ctggagaaga gaataatgag
    15121 cgacatgagc gaactgagcg accgcccctg gctgaagtcc tattcccccg gcgtccccgc
    15181 cgatatcgat ccctcccagt acgagtcgct cgtgcagctg atggaggaaa gcttcaagca
    15241 gtacgccagc cgcccggcct acagcttcat gggcaaggag gtcactttcg gccagaccga
    15301 ctccctcagc cgcgcgcttg ccgcctatct gcagagcctg ggcctcgcca ggggagaccg
    15361 tgtggcgatc atgatgccca acgtgccgca gtacccggtg gcggtggccg ccatcctgcg
    15421 tgccggtttc gtggtggtca acgtcaaccc gctgtacacc ccgcgcgaac tcgaacacca
    15481 gctcaaggac tccggctcca aggcgatcgt catcatcgag aacttcgcca gcacgctcga
    15541 gcagtgcatc gccaacacgc cggtcaagca cgtcatcctg tgctcgatgg gcgaccagct
    15601 cggcctgctg aagggcgcgc tggtcaacta cgtggtgcgc aacgtcaaga agatggtgcc
    15661 ggccttcaac ctgccgggag cggtgcgctt caacgaggcg atcgccctgg gcacgcgcgg
    15721 ctcgttcaag aagccggaga tcaaggccga cgacgtcgcc gtcctgcagt acaccggcgg
    15781 caccacgggc gtttccaagg gtgccgtgct cctgcaccgc aacatcatcg ccaacgtgct
    15841 gcagtcccag gtgtggaacc agccggtgat ggacagggtg ccggccaacg agcaatcgac
    15901 cagcgtctgc gccctgccgc tgtatcacat cttcgccttc acggtgggca tgatgctgtc
    15961 gatgcgcacc ggcggcaagc tgatcctgat ccccaatccg cgcgacctgc cggcggtgct
    16021 caaggaactg tccaagcaca ctttccacag cttcccggcc gtgaacacgc tgttcaacgg
    16081 cctggccaac catcccgatt tcgacaaggt caactggaag aatctcaagg tgtccgtggg
    16141 tggcggcatg gcggtgcagg gggcggtcgc caagctctgg ctggagaaaa ccggctgccc
    16201 gatctgcgag ggttatggcc tgtcggaaac ctcgccctcg gccagctgca atccggtcac
    16261 cagcaaggag tacaccggca ccatcggcgt gcccctgtcc agcacttaca tgaagctgct
    16321 ggacgacgag ggccacgagg ttgcgccggg ccagcccggc gagatcgcga tcaagggccc
    16381 gcaggtgatg gcgggctatt ggcagcgtcc cgacgagacc gccaaggtca tgacgcccga
    16441 cggtttcttc aagagcggcg acatcggcgt cgtggatgaa cgcggttact tcaagatcgt
    16501 cgatcgcaag aaggacatgg tgctggtcag cggcttcaac gtctatccga acgaagtcga
    16561 ggaagtggtc gcgggcctgg acggggtgct cgaatgcgcg gtggtcggcg tggccgacga
    16621 caagaccggc gaagcggtga agctcgtgat cgtcaagaag aaccccgacc tcaccgaagc
    16681 ccaggtgcgc gagttctgca aggcgaacct gaccggctac aaacagccga aggtgatcga
    16741 gttccgcacc gacctgccca agacacccgt gggcaagatc ctgcgtcgtg aattgcgcga
    16801 caagaagtga accggggctt gtcattgcac gaaggttttg cacaaggaag tgatcgactt
    16861 caagctcagc ccattcgggc gtcataacta ctacggcgtg gatatcaagg agtgattcac
    16921 tcgaggaacg ccacgcgttt ttcgaccagt tgagataagt aagcgcggcc aaacgccgcg
    16981 ctttttcttt gcaacgccgc cgctgggccg aatcgatgca ccgctttctt gatcttcgac
    17041 aattttcttg ctggacggta gctctcaatg agagtgagtc gcattacagt tgccttgact
    17101 catcaaaagg aaaagcaacg tgacgaacat gacctcggca gcagccgcca atttgaccgg
    17161 cgccgcatcg cagacacgtc cctgggcctt ggcggccggc ctttgttggg ccacttcggc
    17221 cctcgcggcc ggtggccacc atgccctgga cgacgccgtc atcctcgagc cgggcaattg
    17281 ccaggtggag acctggttga gccgttcgga cgaccgccag cggctgctgc acgtcggtag
    17341 cggctgccgc gtcggtccgg tggaagtggg agtggcggcg gaacatgccc gccagtcagg
    17401 cgcgagcgac accggctacg ggctgcaggc gaaatgggcc accgaattgc tgcccgggtt
    17461 caacgccggc gtctcgctgt ccaccggatg gcaggcccat gtacggcccc gctaccaggg
    17521 cagcacagtg tccggcctgt tcagctggtt tccccgcgac gacctggcct tccatttgaa
    17581 cctgggccgt gacttcgtcc atcgcgacga agaccaggac cgctcgggcg tctcggtgga
    17641 atggacggcc cgccccggct ggtccatcac cgccgagcgc tatcttgaag cgcaaacgca
    17701 cttcgtgcgc gccggggtgc gctgggcgat cagcgaggcc tggagtgtcg acttgagccg
    17761 cgcccaccgg ctgcgcggtc ccggcgagtc caactggacg ctcggcgcga cctggcagtt
    17821 cccccggcct tgatcagagt ctcgccagcc gttcgcggtt cggctggtgg acgacgccgc
    17881 gctcggtgat gagcgcggtc accagctcgg ccggtgtgac atcgaaggcc gggttgcgca
    17941 ccgagacgcc gtccgccgcc caccggtagt cgcgaaagcc ggtgacctct tcgctcgcgc
    18001 gttcctcgat gggaatcgcc gcgccctcgg caatggacat gtcgatggtg gaggtggggc
    18061 aggccacgta gaaggggatg cgatgtcgat gcgcgagcac cgccaccatg tacgtgccga
    18121 tcttgttggc gacgtcgcca ttggccgcga cacgatccgt gccgacgatc accgcatcga
    18181 tctcgccgcg gctcatgagg aatccggaca tgttgtcggt aatgagcgtg accggaatgc
    18241 cttcctgcac catctcccac gccgtcaggc gcgcaccctg cagaaacggg cgggtttcgc
    18301 cggcgacgac cgagatgcgc tttcccgcct ccacggctga gcgaatcacg ccgagtgccg
    18361 tgccgtggcc ggccgtggcc agcgcgccgg cgttgcagtg cgtgagcaca cgcgcgccgt
    18421 cggccaggag ggccgcaccg tgttcgccca tcgcacgatt gacccggatg tcttcggcga
    18481 aaatctcatg ggcctcgtcc agcagcgcct gcgcctgcga cgccgcaggc tgcgcagcga
    18541 tcgattgcca gcgcatgcgc atgcgcctga gtgcccagaa caggttgacg gcggtgggcc
    18601 ggctcgccgc cagcacggcg aaggcgcgct ccatcagaac ggtgaagtca gaagccggcg
    18661 catcttgctg gcgcaacgct tccaacgcga tcccgtacgc cgccgcgcag ccgatggcgg
    18721 gtgcgccacg cacgaccatg tcgcgaattc cgtgtgccac cgcctcggcg ctgtcatacc
    18781 gcacgtacct gaactcggcc ggcaggacac gctggtcgat catttccagc tgcccgttgc
    18841 accagcgcag cgtttgcaca ccgttccctt gcgacgaact catggttcgg cctgctcccc
    18901 ttgcgcatgt tggccgtagg tcttgaactt ctccatcacc tcggccatct gggcatccga
    18961 cagacgacac ggttcggcga tttgccgggc gcgccagtac tgctcgcaca ggctctccac
    19021 ttcgatgcag agcgcgacgg catggtccag atcgcgcccg gtggcgatca tcccgtggtt
    19081 cgccaacagg caggcgcgcc gatcctggag tgcggccagc gcacggtcgg acagctcctg
    19141 cgtgccgaac aaggcgtagg gcgcgcagcg gatcgtggtg ccgcccgccg cggcgatcat
    19201 gtagtgaaag ggcgggatct ccaggccgag acaggccagc gaggtggcga agggcgaatg
    19261 cacgtgcacg atcgcattaa cgtccggccg ccactcgagg atgtcgcgat ggaaacgcca
    19321 ttcgctggag gggcgtcccg ccccgatgac ggcaccgttg gccgcgagca gcaccatgcc
    19381 ctgcggggtc agttcacgcg cgggcacgcc cgacggcgtg acgagaatgc gatccccgca
    19441 gcgcacgccc acgttgcccg aggtgccgcg attcaggccg cgcccggcaa gctcgagcgc
    19501 ggcctggacc atggcgcggc gcagcttggc ttcgccctcc atttcatcct gcattcttga
    19561 ccccaaggac acggccggcc accgcgtcga gccgcgcgac gagcgccggg tcccgcacat
    19621 cgggcgccgt gatcaaggcg tgctccagtg cgtggcgaca cacgcaagcc ggcgccccgc
    19681 cgtccgccgc gatgtggggt gccacgtgcc gcacaagcga gcgcgccttc tcggcgttgg
    19741 ccagcagcac ctggatgatc tggttgaccg tcaccgcatc gtggtccgga tgccagcagt
    19801 cgaagtccgt gaccatcgcg accgtggcgt agcacatctc cgcttcgcgc gcgagcttgg
    19861 cctccggcat gttggtcatg ccgatcacgt ggcagcccca ggtgcgatat agctccgact
    19921 cggccagcga cgaaaactgc ggcccttcca tcaccaggta agtgccgccg cgcacaaccg
    19981 gtatacccgc ctcgcgggcc gccgcctgga ggtgatcgcc caggcgccgg cacaccgggt
    20041 gcgcgagcga cacatgggcc accaggccgg tgccgaaaaa gctcttgttg cgcgcgaagg
    20101 tgcggtcgat gaactgatcg acgatgacga aggtgccggg cggcaactcc tcacgcaggg
    20161 aacccaccgc gctgaccgag atcaggtcgg tgacgccgct gcgcttgagc gcatcgatgt
    20221 tggcgcggaa gttgatctcg ctgggcgcaa tgcgatggcc gcggccatgc cgcgggagga
    20281 acaccatggg ctggccgttc aggtcgccgt acagcaactc gtcggacggc tcgccgaacg
    20341 gcgaggccac cttggcccag cgcttgttcg acaggccgtc gatgtcgtag accccgctgc
    20401 cccccagaac gccgatcacc ggcgcgaccg caattcgcga attcacctca gtcctccttc
    20461 gcgccgcgcg ccacccgcag cgtctgagtt cgcgcggcca gtccagggcc accgcggaac
    20521 cggctttgcc gggccgctgg tggcgccccc ttgaggggga ggcgccgacg gcgcttcggg
    20581 ggtggtttca tttcaaccac ccccgcctgc gaaagtacca catcggcccc agcgccgagg
    20641 ccagcatcaa cgccagggcg aacggatagc cggcctgcca gtccagctcg ggcatgaact
    20701 tgaagttcat gccgtagatg ctggcgatta gcgtcggcgg aagcagtgca acggcggcca
    20761 cggagaagat cttgatgatc ttgttctggt tgatgttgat gaa
//
LOCUS       DASWIY010000047         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2991645, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000047 DASWIY010000000
VERSION     DASWIY010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2991645"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..846
                     /locus_tag="VGA59_01880"
     CDS             61..846
                     /locus_tag="VGA59_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9718454.1"
                     /translation="MKHLELRYDAGVATVTLNRPEVRNAFNDEVIAELTAVFAELDDR
                     AEVRCIVLAGNGTAFCAGADLNWMKRMAGYTRDENLADAAALANMLRVIYRCPKPTIA
                     RVQGDVYAGGMGLVAACDIAVSVDSAQYCLSEVKLGLIPATISPYVIRAMGERAAHRY
                     FLTAERFSAAEALRIGFVHEVVAAGALDAKVAELAQALVQAGPAALKACKALVQDIGG
                     RNITQLLIDRTVQGIADIRSSDEGREGIQSFLGKRKPGWLARG"
     gene            850..1482
                     /locus_tag="VGA59_01885"
     CDS             850..1482
                     /locus_tag="VGA59_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX9718455.1"
                     /translation="MDAWRHLIDTPQLLALAAALGWASGVRLYATVFLVGLAGYMGWI
                     DLPQGLRVLQHPGVLAAAGFMAFTEFFADKIPFVDSAWDMVHTVIRIPAGAALAAGAL
                     GADSQAMGWIAAILGGSLAATSHTAKLTTRAAVNSSPEPFSNVAVSLLEDGFVVFMLW
                     LSATHPVIFAIALALTLILAVVLMVVLFKFLRAVVLRLREFFGARSIRSP"
BASE COUNT          278 a          566 c          636 g          321 t
ORIGIN      
        1 ccgagccgaa gttcggcatc ttccggatgt gagcgaggcc cccttttcga aaggcaatcg
       61 atgaaacacc tggaactgcg ctatgacgcc ggcgtggcca ccgtcacgct gaaccggccc
      121 gaggtgcgca acgccttcaa cgacgaggtc atcgccgagc tgacggccgt gttcgcggag
      181 ctggacgacc gcgccgaggt gcgctgcatc gtgctggccg gcaacggcac ggccttctgc
      241 gccggcgccg acctgaactg gatgaagcgc atggccggct acacgcgcga cgaaaacctg
      301 gccgacgccg cggcgctggc caacatgctg cgcgtgatct atcgctgccc caagccgacc
      361 atcgcgcgcg tccagggcga cgtgtatgca ggcggcatgg ggctggtggc ggcctgcgac
      421 atcgcggtgt cggtcgattc cgcgcagtac tgcctgagcg aagtgaaact gggactgatc
      481 ccggccacca tcagcccgta cgtgatccgg gcgatgggcg agcgcgcggc ccatcggtat
      541 ttcctcacgg ccgagcgctt cagcgccgcc gaggcgctgc gcatcgggtt cgtgcacgaa
      601 gtcgttgccg ccggcgcact cgatgcgaag gtcgccgagc tggcgcaagc gctggtgcag
      661 gccgggccgg ccgcgctcaa ggcctgcaag gcgctggtgc aggacatcgg cggtcgcaac
      721 atcacgcagc tgctgatcga tcgcacggtg cagggcatcg cggacatccg ctcgagcgac
      781 gagggacgcg aagggatcca gtcctttctg ggcaagcgca agcccggctg gctggcgcgg
      841 ggctgagcca tggacgcctg gcgccacctg atcgacacgc cccagctgtt ggccctggcg
      901 gcggcgctgg gctgggccag cggcgtgcgg ctgtacgcga cggtgttcct cgtgggcctg
      961 gcgggctaca tgggctggat cgacctgccg caggggctgc gggtgctgca gcatccgggc
     1021 gtgctggcgg ccgccggttt catggccttc accgaattct ttgccgacaa gattccgttc
     1081 gtggattcgg catgggacat ggtccacacc gtgatccgca ttcccgccgg agcggccctg
     1141 gcggccggtg cgctcggcgc cgacagccag gccatgggct ggatcgcggc gatcctgggc
     1201 ggcagcctgg cggcgaccag ccacaccgcg aagctgacca cgcgcgcggc cgtgaacagc
     1261 tcgcccgagc cgttctcgaa cgtcgctgtg tcgctgctcg aggacggttt cgtcgtcttc
     1321 atgctgtggc tgtcggccac ccatcccgtc attttcgcca tcgcgctggc gttgactctg
     1381 attctggccg tcgtgctgat ggttgtgctg ttcaagttcc tgcgcgcggt ggtgctgcga
     1441 ttgcgcgagt tcttcggtgc caggagcatc cgttcgccgt aaggggctgg gggttagggt
     1501 gtcattgcgg gcttgacccg caatccatga attcatggat cccggatcga gtccgggatg
     1561 acagccctgg tgtttgatgt cgaagggtgc cagatgatgt cattgcgggc tgggctcgca
     1621 atccatgaat tcatggatcc cggatcgggc ctgccccgga ccccggatca ggtccggggc
     1681 aggcctgatc cggggtccgg gatgacagcc agggataatt ttggaaggtg atgatgttta
     1741 acaagatact gattgcaaat agaggggaga tagcctgccg cgtcgccgcg accgcgcgcc
     1801 g
//
LOCUS       DASWIY010000048         3363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2991874, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000048 DASWIY010000000
VERSION     DASWIY010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2991874"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VGA59_01890"
     CDS             complement(<1..814)
                     /locus_tag="VGA59_01890"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX9718456.1"
                     /translation="MAVAKPLPKIATGIAGFDDLSRGGLPRRRTTLLKGGPGSGKTVF
                     ALQCLVNAARQRQDCGLFVAFEESSRQILANAATFDWGLPALTKGKLFFLDARLSPEV
                     VHSGEFDLRGMLAMLKAKKDELGAEWIVFDGIDVLLTLLRDPAAEMREIYRIRDWLAD
                     NGLSAIITAKTEGGLSDAVTYDFMQFMVDCVVQLARRLEQGVSLHRLQITKYRGSDFV
                     AGEFPVGFGASGMEVAPPEPAEIRHLASTVRISTGFERLDKMLGGGLLRGSST"
     gene            complement(942..1082)
                     /locus_tag="VGA59_01895"
     CDS             complement(942..1082)
                     /locus_tag="VGA59_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEX9718457.1"
                     /translation="MNITLSLAPLISLVAGILILVVPRMLNYIVAAYLIVVGIIGIFR
                     LG"
     gene            1241..1756
                     /locus_tag="VGA59_01900"
     CDS             1241..1756
                     /locus_tag="VGA59_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718458.1"
                     /translation="MRPFPRRILQILAAMFALNAVAVAADAEGERGSRFNLHVYGFSY
                     HPDRKGTRESGLNNELNLGIGLNYTLRENERHVSFVEFGGFRDSGNHTATVAGVGYQY
                     KFGKRWRLGGALLTVLSPTYNDGRLAFAVLPMVTYDFGPVKLNAMYAPRYRGYNEFAV
                     FGIYFSLPFGR"
     gene            1856..2005
                     /locus_tag="VGA59_01905"
     CDS             1856..2005
                     /locus_tag="VGA59_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX9718459.1"
                     /translation="MLFTIAVVLVVLWFLGLVTANTLGGLIHALLLIAVIILLVQVIS
                     GRKVL"
     gene            complement(2060..2797)
                     /locus_tag="VGA59_01910"
     CDS             complement(2060..2797)
                     /locus_tag="VGA59_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9718460.1"
                     /translation="MQQANESSQNLLLAALPAADFERLAGHLERIPMRLGDMLYEPDE
                     QLQHAYFPVSSIVSLHYVMATGASAAVAGVGNEGVVGISLFMGGNTTPSSAVVQTAGV
                     AYRLERRWLKQEFERSGALHALLLRYTQALMTQMTQTAVCNRHHSVEQQLCRWLLVTL
                     DRIPSGQIVMTQELVASMLGVRREGVTDAAGRLQEAGVIRYRRGHITVLERAGLETRA
                     CECYEVVRKEFARLLADVRHRQGARIA"
     gene            complement(2805..>3363)
                     /locus_tag="VGA59_01915"
     CDS             complement(2805..>3363)
                     /locus_tag="VGA59_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9718461.1"
                     /translation="VITAQPGHKLRALLAGARDFISKPFDLAEVRTRIRNTVEVRLLY
                     GKLANANQALERSVEERTAELRESEARYRSLTELASDWYWEQDENGTFTKVSGPVLEM
                     LGLRVEALADNVRALHQDGWNDAERAALQAIIESRQPFLDFVYSRVNADGTQQKYQVS
                     GEPMFNQACRFIGYRGIGVEIFAKL"
BASE COUNT          550 a         1114 c         1109 g          590 t
ORIGIN      
        1 gcgtgctgct gccgcgcaac aggccgccgc ccagcatctt gtcgaggcgc tcgaaaccgg
       61 tcgagatgcg cacggtcgag gccaggtgcc ggatttcggc cggctcgggc ggcgccacct
      121 ccatccccga cgcgccgaag cccacgggga actcgcccgc cacgaaatcc gagccgcggt
      181 atttcgtgat ctgcagccgg tgcagcgaaa ccccttgctc caggcgccgt gcgagctgca
      241 ccacgcaatc gaccatgaat tgcatgaaat cgtaggtcac ggcatccgac agtccgcctt
      301 cggtcttcgc agtgatgatg gcgctcaacc cgttgtccgc cagccaatcg cggatgcggt
      361 agatctcgcg catttccgcg gccgggtcgc gcagcagcgt caacaggacg tcgatgccgt
      421 cgaacacgat ccactcggcg cccagctcgt ccttcttcgc cttgagcatg gcgagcatgc
      481 cgcgcaggtc gaattccccc gagtggacca cctcgggcga caggcgcgcg tcgaggaaga
      541 agagcttgcc cttcgtcagg gcgggcaggc cccagtcgaa ggtcgcggca ttggcgagga
      601 tctggcggct gctctcctcg aacgcgacga acaggccgca atcctgtcgc tggcgcgcgg
      661 cgttgaccag gcattgcagc gcgaacacgg tcttgcccga gcccgggccg cccttgagga
      721 gcgtggtgcg ccggcgcggc agcccgcccc ggctcaggtc gtcgaaaccc gcgatgccgg
      781 tggcgatctt gggcaagggc ttggcaactg ccacccgtca catactaccc ttgtatcggg
      841 tgctcatgtc cggtaccgga cgtacggcaa gccctgcggc acgcgcctgg ggggggcgac
      901 aagtgcggtg acacccattg ccacccgcgg agggtgcccc ctcagccgag gcggaagatg
      961 ccgatgatgc cgacgacgat gaggtaggcc gcaacgatgt agttcagcat gcgcggcacc
     1021 accaggatca gaattccggc caccagcgat atcagcggcg ccaggctcag agtgatgttc
     1081 atgcggtttc ctttgtgtag gagtgcaaag ggcgttggtg cgcgatgctc gccaatgcac
     1141 gggcctacgt atgttcgcca gcgcactggg gaaatgactg attgtgcctg cgccgcagag
     1201 ggcacacact cgcggcccct tggtgagcaa gggggtgccc atgagaccct tccctcgccg
     1261 gatcctgcag attctcgccg cgatgttcgc gttgaacgcg gtcgcggtcg cggcggacgc
     1321 cgaaggcgaa cgcgggtcca ggttcaatct gcacgtctac ggtttttcgt accaccccga
     1381 ccggaaaggc acgcgcgaaa gtggcctgaa caacgagctc aatctgggca tcggcctcaa
     1441 ctacacgctg cgcgagaatg agcgccacgt cagtttcgtg gaattcggcg gtttccggga
     1501 ctcgggcaac cacacggcga cggtcgccgg ggtcggctat cagtacaagt tcggcaagcg
     1561 ctggcgactt ggcggcgcgc tgctgaccgt gctcagcccg acctacaacg acggccggct
     1621 cgcttttgcc gtgctgccga tggtcactta cgatttcggc cccgtgaagc tgaacgcgat
     1681 gtacgcgccc cgctaccgcg ggtacaacga gttcgccgtg ttcggcattt atttcagcct
     1741 gccgttcggc cgctaggcgc atcgccgcgg acccggcgcc cgtatgtcgg ccagcgcacg
     1801 gaacagcgcc ggggccaggg gcaccatcga cgcatcgcgt caatggaatg caggcatgct
     1861 attcaccatc gccgtcgtgc tggtcgtact gtggttcctg gggctggtca ctgccaatac
     1921 gctgggcggc ctcatccacg cgctgctgct gatcgccgtc atcattcttc tggtgcaggt
     1981 catttccggg cgcaaggtgc tgtgaacagc gtgtcctagg ccgctgcgcc ggccggtcac
     2041 gagggcgaag aggaccgttc taggcgatgc gggcgccttg ccggtggcgc acgtcggcca
     2101 gcagccgggc gaactccttc ctgaccacct catagcactc gcaggcgcgt gtttccaggc
     2161 cggcgcgctc cagcaccgtg atgtggccgc ggcggtagcg gatgacgccg gcctcctgca
     2221 gcctgccggc ggcatccgtg acgccttcgc gccgcacacc gagcatgctt gcgaccagtt
     2281 cctgcgtcat gacgatctgg cccgagggga tgcgatcgag ggtcaccagc agccagcggc
     2341 acaattgctg ctccacggaa tggtgccggt tgcacacggc ggtttgcgtc atctgcgtca
     2401 tcagcgcctg ggtatagcgc agcagcagtg catgcagggc gccggagcgc tcgaactcct
     2461 gcttcagcca gcggcgctcc aggcggtacg cgacacccgc ggtctgcacc acggccgagc
     2521 tgggcgtggt gtttccgccc atgaacagcg aaattcccac caccccttcg ttgcccacgc
     2581 ccgccaccgc ggcagacgcg ccggttgcca tgacgtagtg cagcgagacg atggacgaga
     2641 ccgggaaata ggcatgttgc agctgctcgt cgggctcgta cagcatgtcg ccgagtcgca
     2701 tcggaatgcg ttccagatgc ccggcgaggc gctcgaaatc cgccgcgggc agtgcggcga
     2761 gcagcaggtt ctggctcgat tcgttggctt gctgcatgac agtcctacag cttcgcgaag
     2821 atttcgacgc cgatgccgcg gtagccgatg aagcggcacg cctggttgaa catcggctcc
     2881 ccgctcacct ggtatttttg ctgcgtgccg tcggcattga cgcggctgta gacgaaatcg
     2941 aggaacggct gccgggattc gatgatggcc tgcagcgccg cgcgctccgc gtcgttccag
     3001 ccgtcctggt ggagggcgcg cacattgtcc gccagcgcct cgacccgcag cccgagcatc
     3061 tccaggaccg ggccggatac cttggtgaat gttccgttct cgtcctgctc ccagtaccag
     3121 tccgaggcaa gctccgtcag gctgcggtag cgcgcctcgc tttcgcgcag ttcggcggtg
     3181 cgctcctcga ccgagcgctc cagcgcctga ttggcgttgg cgagtttgcc gtagagcagc
     3241 cgcacctcca ccgtgttgcg gatgcgcgtc ctgacctcgg ccaggtcgaa cggcttgctg
     3301 atgaaatccc gggcgccggc gagcagcgcg cgcagcttgt ggccgggctg ggcggtgatg
     3361 acg
//
LOCUS       DASWIY010000049        10535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2993786, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000049 DASWIY010000000
VERSION     DASWIY010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10535
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2993786"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VGA59_01920"
     CDS             complement(<1..399)
                     /locus_tag="VGA59_01920"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9718462.1"
                     /translation="MLNRLQATTLILGALAGLFALPGGAVAQAFPIKPVRMIVPYPPG
                     GGTDGLARVLVERMSEHLGQRVIIENIGGGGGTVGSATAAKAAPDGYTIMMGTLGTHS
                     IAPTLYPKLGYNVARDFKPIANFAYLTNYVV"
     gene            complement(435..1046)
                     /locus_tag="VGA59_01925"
     CDS             complement(435..1046)
                     /locus_tag="VGA59_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9718463.1"
                     /translation="MKPMFCEEACRIVAGIAKDDVVVITMSTMKIFPGVAPQANYVSC
                     VPLMGGAASLGLGIAVARPDVRVWVLDGDASLLMELGSLVTVAEAMPKNLVHLLFNNK
                     VQYGGTANLASPRAGAVDFCAMARAAGYPMAVEIDSAANMAATLSALPRDAGPVFGCV
                     RVEPDPGYYTATTPQREIPDNQITRMGDEARRVRQVLQTSRGH"
     gene            complement(1069..2202)
                     /locus_tag="VGA59_01930"
     CDS             complement(1069..2202)
                     /locus_tag="VGA59_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX9718464.1"
                     /translation="MAKIQSIQVAVARVPLDKVTSFATRTVMARDYALVKVRSEAGHE
                     GIGFCYVGSSGGPLAIAAVQELLAPLLLGQNSTRTEGLWQEMYRESVLQGRAGTVMRG
                     ISILDNALWDLNARAAGLPLSQYLGCWTTDAVPAYASGGYYLDGKTPDHLADEVRGYV
                     AAGFKAVKIKVGRHSPREEEARVRAAREAIGPDIHLMLDANNAWADLPTALRYMERFE
                     PYDPYFIEEPFSPDDIDNHARLAERTKILVATGEIEVGRWRFKELLDKGAAQILQTDA
                     AVCGGITEWRRIASMADAYGITICPHWFHDLHVHLVAAAPNARYVEYFLDDQVLNFKR
                     LINRQLEMRDGNLQVPTGAGLGFEFDEDAVKRYALGDGWVNCR"
     gene            complement(2237..3211)
                     /locus_tag="VGA59_01935"
     CDS             complement(2237..3211)
                     /locus_tag="VGA59_01935"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9718465.1"
                     /translation="MSRTRRFLLAVAWGILGLSHGVAAWAQEFPSKPVHVLVGFAAGG
                     PTDILARLVGERLSQMWQQPVVVENRAGAGGMIALEAVTRAPADGYTVGVLNLNHVVA
                     QELLAKPSFSIEKDLVPVIGLARQGNVLVVNPSIPARNTAELISYVKSMPGKVSYASG
                     GNGSPAHLAGELFKLTAGVDMTHVPYKGAAPGLQDVAAGHVALMFAAAPPALQLIKAG
                     KLRALAVTSDTRMAQFPDLPTLAESGIAVDVRDWQGLVVPAGTPGAIVSKLNADVLKV
                     LGSPDFRARVLSLGGEVAGGTPADFAAQIRSETLKWRKVVKEAKISGN"
     gene            3354..4094
                     /locus_tag="VGA59_01940"
     CDS             3354..4094
                     /locus_tag="VGA59_01940"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9718466.1"
                     /translation="MSTNSRLARYRVIADELAAEIRAGTYPLGSNLPVEHDLAERFRT
                     SRQTVREALRVLADKSLIVRRAGFGTTVINTGSRALFVLSLGNLGQLLSYPEGVVRRH
                     VSSGPYVADEATAALLGCEVGTPWVRIRAIRFEAGHDEPLCWVDIHLAPQFASVAKLR
                     GAERLPVVEQIEKLFGQTVESAEVDFSVSRVPAEIAAELGVEAGSPALTVVRRYLGSD
                     GDPLEITISTHPENRYTHRMKLRKARST"
     gene            4190..4438
                     /locus_tag="VGA59_01945"
     CDS             4190..4438
                     /locus_tag="VGA59_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9718467.1"
                     /translation="MITAAQLRAARALLGMDQRALAEASGLSLPTIQRMEASEDVIRG
                     QVESLMKLIAALEGAGVELINQGAVSAGGGRGVRLKSR"
     gene            4471..6483
                     /gene="hyfB"
                     /locus_tag="VGA59_01950"
     CDS             4471..6483
                     /gene="hyfB"
                     /locus_tag="VGA59_01950"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEX9718468.1"
                     /translation="MALTVLLSCVGVLLGTGVAAVVGARKSVTTLFVYSIALTVSTVA
                     LAVAVAVLATDASSIHTAELPIGLPWLGAHFRLDGLSALFLVVVNLGSFTASLYGLGY
                     GRHEPAPGRVLPFVPVFLAGMNLVVLADDAFTFLLTWEFMSVSSWALVMAHHREGDNA
                     RAGYIYLLMASFGTLALLLAFGLLAGAQGGYAFDTVRTAAPSPGLSAAVLALALIGAG
                     SKAGLVPLHVWLPLAHPAAPSHVSALMSGVMTKVAVYGFVRIVFDLLGPPLWWWGAVV
                     LTLGGISAVLGVLYALMEHDLKRLLAYHTVENIGIIFIGLGLALSFQANDMRQPAALA
                     LTAALFHVFNHSVFKSLLFFGAGAVLRATGERDMERLGGLIQRMPLTAIAFLAGSVAI
                     SALPPFNGFVSEWLTFQAVLLSPRLPQWLLKFTVPAVGAMLALSAALAAGCFVKAFGA
                     TFLGRPRTPAAEHAQETDAFSLSAMFLLVLVCLLAGILPGYFIDALAPVTQLLLGAQL
                     PPQEQLKWLTIVPIAESRSSYNGLLLFVLIAAAGWLAAYVIHRWASHALRRSAAWDCG
                     FPEARPVTQYTAGSFAQPIRRVFGTLAFHAREEVTIPGPGQMQPAQLRVHMRDRVWDA
                     FYAPIEGMVGFAADRLNQVQSWTIRAYLTMVFTSVVLLLMVLAVWH"
     gene            6474..7430
                     /locus_tag="VGA59_01955"
     CDS             6474..7430
                     /locus_tag="VGA59_01955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX9718469.1"
                     /translation="MALIHDALVQLVQLLLVLAIAPLLTGYIRKVKAYLLRRRGPPVL
                     QPYRDLLKLIRKEAVLAENASWIFRTAPYAIFATTLIAAALVPTFATGLLFSPAADLI
                     AIVALLGTARFALALAGLDVGTSFGGIGSSREMMIASLAEPAMLMIVFTLSLLAGSTQ
                     LSTVAVFVQGPSIGLRVSLAMALIALIMVAIAENSRIPVDNPATHLELTMIHEAMILE
                     YSGRHLAVIEAAASLKLLLYFSLVSCMFVPWGIATADGDPAAFAIGLVSYITKLAMGG
                     FLLAVFETATAKMRVFRVSDFLGAALMLGLLATLLLFVSQSA"
     gene            7427..8092
                     /locus_tag="VGA59_01960"
     CDS             7427..8092
                     /locus_tag="VGA59_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718470.1"
                     /translation="MMDRLALDVAHLFAGGLVLVSFMLLYQDRMFGLLNTFALHALVV
                     SLSVAWQAHIQSAPHLYITAAIALVVKAIIIPVALHRIIVRLGIHRTIDTVGGIGQTM
                     LAGIGLVALSVMVMLPITAEAGTLTREDLAFSLSVILLGLLMMISRRNAVSQIIGFMS
                     IENGLILAATAAQGMPLVVEISVAFSIIIALIVVGIFLFRIRERFDTMDVHALERFRG
                     GQA"
     gene            8089..9537
                     /locus_tag="VGA59_01965"
     CDS             8089..9537
                     /locus_tag="VGA59_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HEX9718471.1"
                     /translation="MNDPGPLLLNAVLLVPVFGAALLALFPDDHLTGRLNALTSLLTF
                     LASTSLFFFRPETGSFLIVDDVNVVFIVLTTFVGFTTSVFSSTYIAHELETGRLTPLY
                     LRFYHAMYQTLMFAMNLALLANNMGLMWVAIELATLTTVLMVGLYRTPAAIEAAWKYF
                     ILGSVGIALALFGTLLVYLAARSVVGEGLDAMAWNVLVANAAAFDPHLLNLAFVFLLL
                     GYGTKVGLVPLHAWLPDAHAEGPTPISAVLSGLLLNVALFAVLRFKMIMSGNPGALAP
                     GPLMVTLGLLSVLFSAFMLYRRHDIKRMFAYSSIEHMGIITFAFGMGGPLANFAGLLH
                     MTYHSLTKSAIFFCVGHIAQIKGTQRMSDIRGLTVSHPVLGWGLVLGVITIVGLPPTG
                     IFMTEFLIVTSTFARQPVLAVVLGFGLLVGLGALLLRLNALAFGEAYGKDAPSQASFV
                     PMFAHLALVAAAGIFLPSALVAWFQHVATLLG"
     gene            9543..>10535
                     /locus_tag="VGA59_01970"
     CDS             9543..>10535
                     /locus_tag="VGA59_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX9718472.1"
                     /translation="MTTLRGLIEQGRAIAAHRPWPRALVDIDTWLHAINLLEKGQCTL
                     LGLWGEKKFVHVALIDAEGKDPGILSFECDEQHFPSIGRQHAPAIRLERSLRDLSGLE
                     PEGLPDTRGWLDHGRWPVSRPLGRIAQAAGGFKPQPYQFLAAHGESLHEIPVGPVHAG
                     IIEPGHFRFTASGETIVRLEERLGYVHKGIESLMAGQTIERAAILAGRVSGDSTVAYA
                     LAFARAVEAAQDIEVPVRAVWLRALMAELERLANHFGDIGAICNDAAFAMLHAQCALL
                     REQVLRASDTCFGHRLMMDRVLPGGVRGDLTPECARTLTQLLAHFRKDFPELVEL"
BASE COUNT         1631 a         3451 c         3355 g         2098 t
ORIGIN      
        1 gaccacatag ttggtcaggt aggcaaagtt cgcgatcggc ttgaagtcgc gagcgacgtt
       61 gtacccgagc ttcgggtaga gggttggcgc gatggagtgg gtgcccagcg tccccatcat
      121 gatcgtgtat ccgtccggtg ccgccttggc cgcagtcgcg ctgcccacgg tcccgccgcc
      181 cccgccgatg ttctcgatga tgacgcgctg ccccaagtgc tcgctcattc gctcgacaag
      241 tacgcgcgcc aggccgtcgg tgccgcctcc gggcgggtac ggaacgatca tgcgcaccgg
      301 cttgatcggg aatgcctggg ccaccgcgcc gccgggcagt gcaaacaggc ccgccagggc
      361 tcccagtatc agggtcgtgg cttgtaatct gttgagcatg gggtctcctc tgtctctcgt
      421 catctcttct gtgttcagtg gccgcggctg gtttgcagca cctgccggac acgacgcgcc
      481 tcgtcgccca tccgggtgat ctggttatcg gggatctcgc gctgcggcgt ggtggcggtg
      541 taatagccgg ggtcaggttc cacgcgcacg caaccgaaca ccggccccgc gtccctcggc
      601 aaggcggaga gggtcgccgc catgttcgca gcggagtcga tctcaacggc catggggtag
      661 cctgcggccc gcgccatggc gcagaaatcc actgcgcctg cgcgcggact cgcgagattc
      721 gcggtgccgc cgtactgcac cttgttgttg aacaggaggt ggacaaggtt tttcggcatc
      781 gcttccgcca ccgtgaccag tgagcccagt tccatcagca ggctcgcatc gccgtcgagc
      841 acccacaccc gcacgtcggg ccgtgccacg gcaatgccca ggcccaggga ggccgctccg
      901 cccatgagcg gcacacagct cacgtaattg gcctgcggcg ccacgcccgg aaagatcttc
      961 atcgtggaca tcgtgatgac cacgacgtcg tccttggcga tgccggcaac gatccggcac
     1021 gcttcttcgc aaaacatggg tttcatggaa ggctttctgt tttcgccact agcggcagtt
     1081 cacccagccg tcgccgagcg cgtagcgctt gaccgcgtct tcgtcgaact cgaaaccgag
     1141 cccggcgccg gtgggcacct gcaggttgcc atcgcgcatt tcgagctggc gattgatgag
     1201 gcgcttgaag ttcaggacct gatcgtcgag aaagtattcg acgtatcgcg cgttcggcgc
     1261 ggctgccacc agatggacgt gaaggtcgtg gaaccagtgc gggcaaatcg tgatcccgta
     1321 ggcgtcggcc atgctcgcga tacgccgcca ctccgtgatg ccgccgcaca ccgccgcgtc
     1381 cgtttgcagg atttgcgcag cgcccttgtc cagcagttcc ttgaagcgcc agcggccaac
     1441 ctcgatctcg cccgtcgcga ccaggatctt cgtgcgctcg gcgagccggg cgtggttgtc
     1501 gatgtcgtcg gggctgaacg gctcctcgat gaagtagggg tcatacggct cgaagcgctc
     1561 catgtagcgc agcgccgtcg gcaggtcggc ccaggcattg ttggcgtcca gcatcaggtg
     1621 gatgtcgggc ccgatagcct cacgcgccgc ccgcacccgc gcctcttctt cgcgcgggga
     1681 atggcggccg accttgatct tcaccgcctt gaacccggca gcgacgtagc cccggacctc
     1741 gtccgcaaga tgatccggcg tcttgccgtc caggtagtat ccgccgctcg cgtacgccgg
     1801 aaccgcgtcg gtggtccagc aacccagata ttgggaaaga ggcagtcccg cggcgcgggc
     1861 attcaagtcc cacaacgcgt tgtccaggat gctgatgccg cgcatgaccg tgccggcccg
     1921 cccctgcagg accgactcgc ggtacatctc ctgccacagc ccctcggtgc gggtcgaatt
     1981 ctgccccagc aagaggggag cgagaagctc ctgcaccgct gcaatggcga gcggcccccc
     2041 actgctgccg acataacaga agccgatgcc ttcatggcct gcttcggagc gcaccttgac
     2101 cagggcatag tcgcgcgcca tcacggtacg cgtggcgaac gaggtgactt tgtcgagggg
     2161 aacgcgggca accgcgacct gaatggattg gatcttggcc ataaggggga aaagggttgg
     2221 gagaaatgag cgagcgctag ttgccggaaa tttttgcttc cttgaccacc ttgcgccatt
     2281 tcagggtctc gctcctgatt tgagcggcga agtccgctgg cgtgccaccg gcaacctcac
     2341 cgcccaagct gagcacacgg gcccggaaat ccggcgaacc cagaaccttg agcacgtcgg
     2401 cgttcagctt gctgactatg gctcccggtg tgcctgccgg aacgacaagc ccctgccagt
     2461 cacgcacgtc cacggcgatg ccggattccg ccagcgtcgg cagatcggga aactgggcca
     2521 tccttgtgtc gctggtgacg gccagggcgc gcagcttgcc cgccttgatc agttgaagtg
     2581 ctggcggtgc agccgcgaac atcagcgcga cgtgcccggc cgccacatcc tgcagcccgg
     2641 gcgccgcgcc cttgtagggg acgtgggtca tgtcgactcc ggccgtaagc ttgaagagct
     2701 cgcccgccag atgcgcgggc gagccgttgc cgccggacgc atagctcacc ttccccggca
     2761 tcgacttgac gtacgagatc agttccgccg tgttgcgcgc gggaatcgat gggttgacga
     2821 ccagcacgtt tccttgccgg gcaaggccaa tgaccggaac gagatctttt tcaatgctga
     2881 aggagggctt ggccagcagc tcctgggcga cgacatggtt gagattcaag acccccaccg
     2941 tgtagccgtc ggccggtgcg cgtgtcacag cctccagtgc aatcatcccg cccgccccgg
     3001 cgcggttctc caccaccaca ggctgctgcc acatctgcga caggcgctca ccgacgagcc
     3061 gcgccaggat gtccgtgggg ccgccggcgg caaacccgac gagcacgtgc accggcttgg
     3121 acggaaactc ctgggcccac gcggcaacgc cgtgggacaa acccagaatg ccccacgcca
     3181 cggcaagaag aaaacgacgg gtacggctca tgatgcactt cctttcgagc ggcaacttta
     3241 tcagactttc tgtatttgta catacatata tgctggcatc ctcctctgag attagaagcg
     3301 atgtaaggaa atgaacgtac atttacgggt tagatcaaag cgggatcgca ggtatgagta
     3361 cgaattcgag gctggcacgc tatcgcgtca ttgcagatga actcgccgcg gagattcggg
     3421 cgggcacata cccgctcggc tcgaacttgc ccgtggagca cgacctggca gagcgcttcc
     3481 gcaccagcag gcagacggtg cgcgaggcgt tgcgcgttct cgccgacaag agcttgatcg
     3541 tgagacgtgc cggattcggc accacagtca tcaacactgg cagccgcgcg ctttttgtcc
     3601 tgtcgctggg aaatctcggc cagttgctca gctatccgga aggcgtggtc cgccggcacg
     3661 tgagcagtgg accctatgtc gccgacgagg cgacagcggc tctcctgggc tgcgaagtgg
     3721 gcacaccttg ggttcgaatc cgggccatcc gtttcgaggc cggtcacgat gaaccgctct
     3781 gctgggtcga tattcacctg gcgccgcaat tcgcttcggt ggccaagctt cgcggcgcgg
     3841 agcgcctccc tgtggtcgag cagatcgaaa aactgtttgg ccaaacggtc gagagcgcag
     3901 aggtggactt ttcggtcagc cgggtgccgg ccgaaatcgc cgcagaactc ggcgtcgaag
     3961 ctgggagccc tgccctcacg gttgtgcgcc gttacctggg ctccgatggc gacccgctgg
     4021 aaatcacgat cagcacgcat cccgaaaacc ggtacaccca ccggatgaag ctgcgaaagg
     4081 cgcgatccac ctgatcccgg accggtcgat acgctcggct tgaactattc gtgttggcgt
     4141 tctaagattg atttgtgagg gttcatacgt cattagtcat gcaacgaaca tgattaccgc
     4201 cgctcaactc cgggctgccc gggccttgct gggaatggac cagcgtgccc tggccgaagc
     4261 ctccggactg tcgttgccga cgatccagcg gatggaagcg agcgaagacg tgatccgggg
     4321 ccaggtggag tccctgatga agctgattgc cgcgctcgaa ggggcgggcg tcgagctcat
     4381 caaccaggga gcagtcagcg ccggcggcgg gcgcggggtt cgattgaagt cgcggtgatg
     4441 tttcgctgaa gcgcgagggg caacgagacg atggctttga cagtgcttct gtcatgcgtt
     4501 ggagttctgc tcggcaccgg ggtggcagcc gtcgtgggtg cccgcaagag cgtgaccacg
     4561 ctcttcgtct attcgatcgc cctcaccgtc agcaccgttg cattggcggt cgccgttgcg
     4621 gtgctggcga cggatgcgag ctcgatccat accgctgaac tgccgatcgg tttgccgtgg
     4681 ctcggcgcgc atttccgcct ggatggcctc agcgccctgt ttctcgttgt cgtgaatctc
     4741 ggcagcttca ccgccagcct gtatggcctc ggctatggcc ggcacgaacc ggcccccggg
     4801 cgcgtgctgc cgttcgtgcc ggtctttctt gcgggcatga atctggtcgt cctcgccgac
     4861 gacgcgttca ccttcctgct cacctgggaa ttcatgtccg tgtcctcgtg ggcgctggtc
     4921 atggcgcacc accgcgaagg cgataacgca agggcagggt acatctacct gctgatggca
     4981 agcttcggca cgctggcgtt gctgcttgct ttcggtctgc tggccggcgc gcaaggtggt
     5041 tacgcgttcg atacggtgcg aacggcagcg ccttcgcccg gcctgtccgc cgcggtgctc
     5101 gccctggccc tgatcggcgc cggatcgaaa gccggcctcg tgcccttgca cgtgtggctg
     5161 ccccttgccc atccggccgc accgagccat gtatcggctc tgatgagcgg cgtgatgaca
     5221 aaggtggccg tctacggctt cgtgcgcatc gtgttcgacc tgctcggccc gccgctctgg
     5281 tggtggggcg ccgtggtgct cacgctgggc gggatcagcg ccgtgctcgg tgtgctgtac
     5341 gcgctgatgg aacacgacct caagcgcctc ctcgcctacc acacggtgga gaacatcggc
     5401 atcatcttca tcgggcttgg actcgcgctg tccttccagg ccaacgacat gcgccagccg
     5461 gcggcgctcg cgctcacggc ggccttgttt cacgtgttca atcactccgt tttcaagagt
     5521 ctgctgttct tcggcgctgg cgccgtactc agggccaccg gcgaacgcga catggagcgc
     5581 ctcggcggcc tcatccagcg catgccgctc accgccattg ccttcctcgc gggctccgtc
     5641 gccatttcgg cgcttccgcc gttcaatggt ttcgtgtccg aatggctgac cttccaggcg
     5701 gttctcctga gtcccaggct cccgcagtgg ctgctgaagt tcacggtgcc ggccgtgggc
     5761 gccatgctgg ccctgtccgc tgcgctcgcc gcgggctgct tcgtgaaagc cttcggcgca
     5821 accttcctcg ggcggccccg aacccccgcg gccgagcacg cgcaagagac cgacgcgttc
     5881 tccctgtccg cgatgttcct gctggtgctg gtgtgccttc tcgccggcat cctgccgggc
     5941 tatttcatcg atgcgctggc gccggtgacg cagctgctgc tcggcgcgca actgccgccg
     6001 caggagcaac tgaagtggct cacgatcgtc ccgatcgccg aaagccggag ctcgtataac
     6061 gggctgctgc tgttcgtcct gatcgcggcc gccggctggc tggctgcgta tgtcatccac
     6121 cgctgggctt cgcatgcgct gcggcgctcg gcggcctggg attgtggatt tcccgaagcg
     6181 cggccggtga cgcaatacac ggcgggcagc tttgcccagc cgatccgccg cgtgttcggc
     6241 acgcttgcct tccatgcccg cgaagaagtc accatcccgg ggccgggaca gatgcagccg
     6301 gcgcagcttc gtgtgcacat gcgcgaccgc gtgtgggacg ccttttacgc gcccatcgaa
     6361 ggcatggttg gctttgccgc cgaccggctc aaccaggtcc agtcctggac gattcgcgcg
     6421 tacctgacga tggtgttcac cagcgtcgtg ctgctgttga tggtgctggc ggtatggcat
     6481 tgatccatga cgcactggtt caattggtgc agctgctgct ggtgctggcc atcgcgcccc
     6541 tgctcaccgg ctatatccgc aaggtcaagg cctacctgct gcggcgccgg gggcctcccg
     6601 tgttgcagcc ctaccgcgac ctgctcaagc tgattcgcaa ggaggcggtg ctggcggaga
     6661 acgcctcctg gatattccgc acggcgcctt atgcgatctt cgccaccacc ttgatcgcgg
     6721 cggccctggt gcccaccttc gccacgggac tcctgttcag tccggcggcg gacctcatcg
     6781 ccatcgtggc gcttctcggt accgcgcgct tcgccctggc gctggcgggg ctcgacgtgg
     6841 gaacgagttt cggcggcatc ggctccagcc gcgagatgat gatcgcctcg ctggccgagc
     6901 cggcgatgct gatgatcgtc ttcacgctgt cgctgctggc cggctcgacc cagctgtcga
     6961 ccgttgctgt tttcgtgcag ggaccctcga tcgggctgcg cgtatccctc gccatggcgc
     7021 tgatcgccct gatcatggtt gccatcgccg agaactcgcg catcccggtg gacaatccgg
     7081 cgacccatct cgaactgacc atgatccacg aggccatgat cctcgagtat tcgggacggc
     7141 accttgccgt gatcgaagcc gccgcctcgc tcaagctcct gctttatttc tcgctcgtct
     7201 cctgcatgtt cgtgccctgg ggaattgcga ccgctgatgg cgatcccgcg gccttcgcga
     7261 tcggcctggt cagctatatc accaagctgg cgatgggcgg attcctgctg gcggtgttcg
     7321 agacggcgac ggcgaagatg cgggtttttc gcgtgtcgga ttttctgggt gcggcgctca
     7381 tgctgggcct gctggcgacc ctgcttctct tcgtgtcgca gagcgcatga tggaccggct
     7441 tgcacttgac gttgcccacc tgtttgccgg cgggctcgtg ctcgtcagtt tcatgctgct
     7501 gtaccaggac cgcatgttcg gcctgctcaa taccttcgcg ctgcacgcgc tcgtggtctc
     7561 gctctcggtc gcgtggcagg cgcacatcca gtcggcgccg catctgtaca tcaccgccgc
     7621 catcgcgctg gtcgtcaagg ccatcatcat tccggtggcg ctgcaccgca tcatcgtgcg
     7681 cctcggaatc catcgcacga tcgatacggt gggcgggatc gggcagacca tgctggccgg
     7741 catcggactg gtggccttgt cggtcatggt gatgctgccc atcaccgccg aagccgggac
     7801 gctgacgcgc gaggacctgg ccttctcact gtcggtcatc ctgctcgggc tgctgatgat
     7861 gatttcgcgc cgcaacgccg tgagccagat catcggcttc atgtcgatcg aaaacggcct
     7921 catcctggcc gcgaccgccg cccagggcat gccgctcgtg gtggagatca gcgtcgcgtt
     7981 ctcgatcatc atcgcgctga tcgtcgtcgg cattttcctg ttccgcatcc gcgagcgctt
     8041 cgacaccatg gacgtgcacg cgcttgagcg cttccgcgga ggccaggcat gaacgatccg
     8101 ggcccgctgc tgctcaatgc cgtcctgctg gtgcccgtct tcggcgccgc gctgctggcg
     8161 ctgttcccgg acgatcatct caccggccgt ctcaacgccc tcacttcact gctcacgttt
     8221 ctcgcctcga cctcgctgtt tttcttccgg cccgaaacgg gttcgttcct catcgtcgat
     8281 gacgtgaacg tggtgttcat cgtgctcacc acgttcgtcg ggttcaccac cagcgtgttc
     8341 agctccacct acattgcgca cgagctcgaa acggggcgcc tcacaccgct ctatctgcgc
     8401 ttctatcacg ccatgtacca gacgctcatg ttcgccatga acctcgcgct gctggcgaac
     8461 aacatgggct tgatgtgggt cgccatcgaa ctcgcgacgc tcaccaccgt gctgatggtg
     8521 ggcctctacc gcacgccggc ggcgatcgaa gcggcatgga agtatttcat cctgggcagc
     8581 gtcggcatcg cgctggccct gttcggcacg ctcctggtct atctcgcggc acgctcggtc
     8641 gtcggggagg ggctcgacgc aatggcctgg aacgtgctgg tggcgaatgc cgccgccttc
     8701 gatccgcatc tgctcaacct ggccttcgtg ttcctgctgc tgggttatgg cacgaaggtc
     8761 ggcctcgtgc ccctgcacgc gtggctgccc gacgcgcacg cggaagggcc gacgcccatc
     8821 tccgccgtgc tgtcggggct gctgctcaat gtcgcgctgt tcgcggtgtt gcgcttcaag
     8881 atgatcatgt ccggcaatcc cggcgccctc gcgcccggcc cgctgatggt cacgctcggc
     8941 ctgctgtcgg tgctgttctc ggctttcatg ttgtaccggc gccatgacat caagcgcatg
     9001 ttcgcctact cgtccatcga acacatgggg atcatcacct tcgccttcgg gatgggcggg
     9061 ccgctggcca acttcgccgg gttgctgcac atgacctatc acagcctgac caagtcggcg
     9121 attttctttt gcgtcggtca cattgcgcag atcaagggca cgcaaaggat gtccgatatc
     9181 cgcggactca cggtttcaca tccggttctc ggctgggggc tggtgctggg cgtgatcacc
     9241 atcgtgggcc tgccgcccac cggcatcttc atgaccgaat tcctgatcgt tacttcgact
     9301 tttgcgcggc aaccggtgct ggccgtggtg ctggggttcg gcttgctcgt gggcctgggc
     9361 gcgctgctgc tgcggctgaa tgctctcgcg ttcggcgaag cgtatggcaa ggacgcgccg
     9421 tcgcaggctt cgttcgtgcc gatgtttgcg catctggcgc tggttgccgc cgccggaata
     9481 tttcttccgt cggcgctggt ggcgtggttc cagcacgtcg ctaccttgct tgggtagcgg
     9541 ccatgacgac gctgcgcgga ctcatcgagc aaggccgggc catcgccgcg caccggccgt
     9601 ggccacgcgc gctcgttgac atcgacacct ggctgcatgc gatcaacctg ctggaaaagg
     9661 ggcaatgcac gctgctcggg ctgtggggag agaagaaatt cgtgcatgtc gcactcatcg
     9721 acgccgaagg caaggacccc gggattctca gcttcgagtg cgatgagcaa cacttcccgt
     9781 cgataggacg gcagcatgcg cccgccatcc gcctggagcg atccctgcga gacctgtcgg
     9841 ggttggagcc cgagggattg cccgataccc gtggctggct ggaccacggc aggtggcccg
     9901 tgagcaggcc gttgggacgt atagcgcagg ctgccggcgg attcaaaccc cagccctatc
     9961 agtttctggc ggcgcacggc gagagcctgc atgagattcc cgtgggtccg gtgcacgcgg
    10021 gcatcatcga gcccggtcac ttccggttca cggcaagcgg cgaaaccatc gtgcggttgg
    10081 aggaacggct cggctatgtg cacaaaggca tcgagtcgct gatggccggg cagacgatcg
    10141 aacgcgcggc gatcctggca gggcgcgttt caggcgacag caccgtcgcc tacgccctgg
    10201 cttttgcccg tgccgtcgaa gcggcgcagg acatcgaggt tcctgtgcgc gccgtctggc
    10261 tgcgtgcgct gatggcggaa ctggagcggc tggccaatca cttcggcgat ataggcgcca
    10321 tctgcaacga cgccgcgttc gccatgctgc atgcgcagtg cgcgctgctg cgcgaacagg
    10381 tgctgcgcgc gtcggacact tgtttcggcc accggctgat gatggaccgc gtcttgcccg
    10441 gcggtgtgcg cggcgacctg actccggaat gcgcgcggac cctgacccaa ctgcttgccc
    10501 attttcgcaa ggacttcccc gagctggtcg agctc
//
LOCUS       DASWIY010000050         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_31827, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000050 DASWIY010000000
VERSION     DASWIY010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31827"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VGA59_01975"
     CDS             complement(<1..855)
                     /locus_tag="VGA59_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9718473.1"
                     /translation="MSNALRPFLHLLLCGALAAASQAFAAGASLVVCTEASPEGFDIV
                     QYTAATTADATAETVFERLIGFAPGSTELIPALAERWEVSADGLSYTLQLRRGVKFHR
                     TAWFTPSREFNADDVLWSLQRQLDPQHPWHKLSVRGFPYAEAMAMAQLIERIDRLDDH
                     RVRFVLKHPEAPFLANLAMGFASIYSAEYAAQLLAQGKSEQLNRLPVGTGPFVFERYA
                     KDAQVRFRGNPDHWAGAPALERLIFAITPEPNVRQQRLRAGDCQIALYPRPVDIPALK
                     ADPALQVLE"
     gene            complement(1047..1373)
                     /locus_tag="VGA59_01980"
                     /pseudo
     CDS             complement(1047..1373)
                     /locus_tag="VGA59_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234010.1"
                     /note="DppABCDF is involved in the transport of
                     dipeptides; also binds heme and mediates chemotaxis to
                     dipeptides; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(1536..>2131)
                     /locus_tag="VGA59_01985"
     CDS             complement(1536..>2131)
                     /locus_tag="VGA59_01985"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX9718474.1"
                     /translation="REIAHDLMHVTLYSEAQDSDPAALAAQTSKTLNASLTQARKVKG
                     VSVAMGSRHSYPVYDDKGQQISAWRERAELRLESADFSALSKLTGEMLRGLKMGGMNF
                     SIADATRKTHEDALLKEAVAAFKARAQLATDALGGSGYKLVNLNLNSGGFQPPMPLRM
                     SAMKGATMMDAAVTPDIEAGTSQVTINADGVIEVQMP"
BASE COUNT          338 a          641 c          752 g          400 t
ORIGIN      
        1 ctcaagcacc tgcaacgccg ggtcggcctt gagcgcggga atgtccaccg ggcgcgggta
       61 cagggcgatc tggcagtcgc cggccctgag ccgctgctgg cgcacattgg gctcgggggt
      121 gatagcgaag atcagccgct cgagcgccgg cgcgccggcc cagtggtccg ggttgccgcg
      181 aaagcgcacc tgggcgtcct tggcgtagcg ctcgaagacg aagggcccgg tgccgaccgg
      241 caggcggttg agttgttcgc tcttgccctg ggcgagcagt tgcgcggcgt actcggcgga
      301 atagatggag gcgaagccca tggccagatt ggccaggaag ggcgcctcgg ggtgcttgag
      361 gacgaagcgc acgcggtgat cgtcgagcct gtcgatgcgt tcgatcagtt gcgccatggc
      421 catggcttcg gcatagggga agccgcgcac ggacagcttg tgccaggggt gctgcggatc
      481 gagctggcgc tgcaagctcc agagcacgtc gtcggcattg aattctcggc tcggggtgaa
      541 ccaggcggtg cggtgaaatt tcaccccccg gcgcagctgc agggtgtagc tgaggccatc
      601 ggcagagact tcccagcgct cggccagcgc ggggatcagc tcggtgctgc cgggggcgaa
      661 gccgatcagg cgttcgaaga cggtttccgc cgtggcatcg gcagtggtcg ccgcggtgta
      721 ctggacgatg tcgaagcctt ccgggctggc ctcggtgcac accaccaggc tggcgccggc
      781 ggcaaaagcc tggctggccg cggcgagggc gccacagagc agcaggtgga gaaacgggcg
      841 cagggcattg gacatcttcg ctgttcccaa actacggcgg tgcgaatcgg gcgtgacggg
      901 ataggccacg gctgacacga aacccggacc ggcagataac cagcccgggt gttcactcag
      961 aggttgtgaa catatcgcaa cgccgcagcg gacgtttgca ggcggccgca gtaggcgagc
     1021 gtttcttcac aaccgctcag gacgggttac tcgacgctca ctccgtagaa ggcgttgagc
     1081 gcgaagggac cgatcttgaa gtcctttacg ctcgtgcgca tcggttgata cacggtggag
     1141 tgggcgatcg gggtgatcgg cacttcgcgc ttgagaatca cctgggcctg ctggtacagc
     1201 gcggtgcgct cggccacgtc ggtggtgcgc ttggccgcct tgatcagctt gtcgtactcc
     1261 gcgaagcacc acttggagaa gttgttgccg tccaccgcat cgcagccgta cagggtgccc
     1321 agccagttgt ccgggtcgcc gttgtcgccg ctccagccga tcagcatggc atccttgtgg
     1381 agacctgaaa atatgcaggg catgtgccgc aggcaagggg catgacccat tcagcgtagc
     1441 aactgcgggc gagttgtacg tgactgtcgc atttaggcaa ttttcgtgga taaggctggc
     1501 aatgcgccgc tatctggcga caacggcgct gcctctcagg gcatctgcac ttcgatcacg
     1561 ccatcggcat tgatcgtcac ctggctggtg ccggcttcga tgtccggcgt caccgcggca
     1621 tccatcatcg tcgcgccttt catcgcgctc atgcgcagcg gcatcggtgg ctgaaagccg
     1681 ccgctgttca ggttcaggtt gaccagcttg tagccgctgc cgcccagggc gtcggtggcc
     1741 agttgcgcac gggccttgaa cgccgccacg gcctccttga gcagggcgtc ctcgtgggtc
     1801 ttgcgcgtgg cgtcggcaat gctgaagttc atcccgccca tcttcagccc tcgcagcatc
     1861 tcgccggtga gcttggacag ggcgctgaaa tcggcgcttt ccaggcgcag ttcggcacgc
     1921 tcgcgccagg cgctgatctg ctggcccttg tcatcgtaga ctgggtaact gtggcggctg
     1981 cccatggcca cgctgacgcc cttgaccttg cgcgcctggg taaggctggc gttgagggtc
     2041 ttgctggtct gcgcggcgag ggcggccggg tcgctgtctt gcgcctcgct gtagagggtg
     2101 acgtgcatca ggtcgtgggc gatttcccgg t
//
LOCUS       DASWIY010000051         5236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_404037, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000051 DASWIY010000000
VERSION     DASWIY010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404037"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VGA59_01990"
     CDS             <1..677
                     /locus_tag="VGA59_01990"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9718475.1"
                     /translation="DRPSTLAVNETRTRRCKEGARRGVTSKETTMVQTLLDRYCRLLA
                     MIMAACLAVMVVMVFGNVVLRYGFNSGIPVSEELSRWLFLWLVFLGAAIAVREHAHMG
                     SELLVERMPAWLQRPTLVLGQLLMLWVTWLMFSGSLEQTRINWDAEAPVTGASMAWVY
                     MTGVVFAVSSGAILLADLWFIVRGELTPMQVLAAHRASEQAQAEAKAGRAALAPATGD
                     VERSAR"
     gene            674..1954
                     /locus_tag="VGA59_01995"
     CDS             674..1954
                     /locus_tag="VGA59_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9718476.1"
                     /translation="MTIVVFLAALIGAVALGIPIAYSLLLSGVALMVHLGNFDSQILA
                     QNVIDGANSFPLLAVPFFMLAGEIMNVGGLSRRIVDLALKLVGHVRGGLGYVTIIAAC
                     LMASLSGSAVADTAALTALLLPMMVRAGHQKARSGGLIASAGIIAPIIPPSIPFVIFG
                     VTANVSIGKLFLAGIVPGMMMGIAIAAAWWLSARSEAVKTPPRANRAETLKALRESAW
                     AMVLPVIVLVGLKFGIFTPTEAAVVAAVYALFVATVIYRELRLDQLYDVFVGAAKTTS
                     IIMFMVAAAAVSAWLIAVANLPAQLIELLQPFLNSPTLLLLMLMSVLLLVGMVMDLTP
                     TILILAPVMVPVVKAAGIDPIYFGVLFVLNGVIGLVTPPVGTVLNVVAGVGRMKIDQV
                     AAGVVPFIIADVVVLGLLVMFPQLVLVPLNFLYR"
     gene            2037..3041
                     /locus_tag="VGA59_02000"
     CDS             2037..3041
                     /locus_tag="VGA59_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9718477.1"
                     /translation="MKRLILKTLVAALAVTAVGFASAQDIKARTLKFATQGSKGHPSV
                     FGMEKFAELVTAKSGGKIKVNLFISGVLGSDQSNISSIQGGTLDMAVMNTGILASVAK
                     ELAIFDFPFLFANEKESDALVDGPVGQKLHGYLQPKGLVGLSYWELGYRHITNSKRPL
                     TKVEDIEGLKLRVIPNPINVAWVKALGANPTPLPFPELYAALEQKAIDGQENPVSVIA
                     SSKFWEVQKHMALTNHQYNPQSVIFSKKIWDTLSAAEKKIISDAAHEATKFQRQQTRA
                     AVGVNLDLLKKSGMNVTELPPAEMAKLREKMKPVIAQFSASVGQGTLNEVTAELAKMR
                     "
     gene            complement(3092..3745)
                     /gene="rsxB"
                     /locus_tag="VGA59_02005"
     CDS             complement(3092..3745)
                     /gene="rsxB"
                     /locus_tag="VGA59_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HEX9718478.1"
                     /translation="MSELAARLHAALPQTQCTRCGYPDCAGYAQAIADGKAEINQCPP
                     GGAQGIARLAAITGRPPLPLNPVNGAEGPRTVAVIDEAWCIGCTLCLDACPTDAIVGS
                     NKLMHTVIERYCTGCELCVPVCPVDCIGMENVTGERTGWKAWSAAQAALALERYQFRQ
                     ERLEREEKEQRDRLGAKALNKLADLPAQTHDSEDAQADRKRAVIEAALARARARRQG"
     gene            complement(3747..5102)
                     /gene="phaZ"
                     /locus_tag="VGA59_02010"
     CDS             complement(3747..5102)
                     /gene="phaZ"
                     /locus_tag="VGA59_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX9718479.1"
                     /translation="MLYQIYEAQRSMMEPFADFAQAASKLYSNPLSPFGQNPFAQRVS
                     ASYDLMYRLWKDYEKPQFGIRTVNVDGTDVAIHERVEIDKPFCELRRFKRFTDNPATL
                     TKLKGQPAVLIVAPLSGHYATLLRDTVRTMLKDHKVYITDWKNARTVPLEQGEFHLDD
                     YVHYVEEFIRHLQGIYGNCHVISVCQPTVPVLAAVSLMASRGEVTPMTMTMMGGPIDA
                     RKSPTAVNNLAMNKSYEWFENNVIYRVPTSFPGAGRRVYAGFLQHTGFVAMNPDRHMS
                     SHYDYFKHLIAGDDASAEAHRTFYDEYNAVLDMDADYYLETIRVVFQEFALVNGTWDV
                     ISDDGDLERVRPEDITETAHFTIEGELDDISGAGQTEAAHDLCYNVPKSRQKHLEVKG
                     AGHYGIFSGRRWRDAVYPQVRDFILQFNEGPRVPVNAGRRSSANGPAAARKSGQQRSI
                     A"
BASE COUNT          879 a         1637 c         1687 g         1033 t
ORIGIN      
        1 gggatcggcc gtccacattg gccgtcaacg agacacgaac tcgccgctgc aaggagggtg
       61 cgcggcgcgg tgtgacttca aaggagacaa cgatggtgca gacattgctc gaccggtatt
      121 gccggctgct ggcgatgatc atggccgctt gcctggcggt gatggtcgtc atggtcttcg
      181 gcaatgtcgt gcttcgatac ggattcaatt cgggcattcc cgtttcggag gagctgtccc
      241 gatggctgtt tctgtggctc gtgtttctgg gcgcggcgat cgcggttcgc gaacatgcgc
      301 acatgggcag cgaactgctc gtcgagcgca tgcccgcctg gctgcagaga ccgacgctcg
      361 tgctgggcca gctgttgatg ctgtgggtca cgtggctgat gttctccggc agcctggagc
      421 agacccgcat caactgggat gccgaggcgc cggtcacggg cgcttcgatg gcctgggtct
      481 acatgacagg ggtcgtgttc gcggtttcga gcggggcgat cctgctcgcc gacctgtggt
      541 tcatcgtgcg cggtgaactg accccgatgc aagtgcttgc cgcccaccgt gccagcgagc
      601 aggcgcaagc cgaggcgaaa gccggtagag ccgcgctggc tcccgcaacg ggtgatgtcg
      661 aacggagcgc gagatgacca tcgtcgtctt cctggccgca ttgatcggcg ccgtcgctct
      721 cggcattccc atcgcctatt cgctgctgtt gtcgggtgtt gccctgatgg ttcaccttgg
      781 caatttcgac tcgcagatcc tggcgcagaa cgtgatcgac ggcgccaaca gcttcccgct
      841 gctggccgtt ccgttcttca tgctggccgg cgagatcatg aacgtaggcg gtttgtcccg
      901 caggatcgtc gatctcgcgc tgaagctcgt gggccacgtg cgcgggggac tcggctacgt
      961 caccatcatc gcggcctgcc tgatggcctc gctgtcgggc tcggccgtgg ccgataccgc
     1021 cgccctgact gcgctgctgc tgccgatgat ggtgcgtgcc gggcaccaga aagcccgttc
     1081 cgggggcctc attgcatcgg caggcatcat cgcgccgatc attcccccat cgattccctt
     1141 cgtcatcttc ggggtcaccg ccaacgtttc catcggcaag ctattcctgg ccggcatcgt
     1201 gcccggcatg atgatgggca tcgccatcgc ggcggcatgg tggctttctg cgcgcagtga
     1261 agccgtgaag acacccccca gggcgaaccg cgccgagacc ctgaaggcgc ttcgcgaatc
     1321 cgcctgggcg atggtgctgc cggtgatcgt gctggtgggc ctgaaattcg gcatcttcac
     1381 gccaaccgag gcggcggtag tcgccgcggt ctacgcgctg tttgtcgcaa ccgtgatcta
     1441 tcgggagctc aggctggacc agctctacga cgtatttgtc ggcgcggcga agacaacctc
     1501 gatcatcatg ttcatggtcg cggctgccgc cgtcagcgcg tggttgatcg cggtcgccaa
     1561 cctgcccgcg caattgatcg aattgctgca gccgttcctg aacagcccga cgctgctgct
     1621 cctgatgctg atgtcggtgc tcttgctggt cggcatggtg atggacttga cgcccacgat
     1681 cctgatcctc gcgccggtga tggtgccggt ggtcaaggcc gccggcatcg atcccatcta
     1741 tttcggggtg ctgttcgtcc tgaacggcgt catcggcctg gtcacgcccc cggtgggcac
     1801 ggtgctcaac gttgtcgcgg gcgtgggcag gatgaagatc gaccaggttg ccgcaggcgt
     1861 ggtgccgttc atcatcgccg acgttgtcgt gctggggctt ctcgtgatgt tcccgcagtt
     1921 ggtcctcgtg ccgctgaatt tcctttatcg ctgaaatatt cccgatccgc tgcggcgcca
     1981 gggcacgccg ccgcagcgat cactttgcat gcgtccctga cccgtggaga aactctatga
     2041 aacgtctgat cctcaagacc ctggttgccg ccctggcggt gacggccgtc ggcttcgcgt
     2101 ccgctcagga catcaaggcg cgcacgctca agttcgcgac ccagggatcg aagggccacc
     2161 cctccgtttt cggcatggaa aagttcgcgg aactcgtcac cgccaagtcg ggcggcaaga
     2221 tcaaggtgaa tctgttcatc agcggcgtgc tcggcagcga ccagtcgaac atttcttcca
     2281 tccagggcgg gacgctggac atggcggtca tgaacaccgg catactggcc agcgtggcga
     2341 aggagctcgc catcttcgac ttcccgttcc tgttcgccaa tgagaaggaa tcggatgccc
     2401 tcgtcgacgg ccccgtgggc cagaagctcc atgggtacct gcagcccaaa gggctggtgg
     2461 ggctttcgta ttgggagctg ggctatcgcc acatcaccaa cagcaagcgc cccctgacca
     2521 aggtggagga catcgaaggc ctcaagctgc gcgtgattcc caacccgatc aacgtggctt
     2581 gggtgaaggc gcttggcgcc aatccgaccc ccttgccctt cccggaactc tatgctgcgc
     2641 tggagcagaa ggccatcgat ggccaggaga atccggtcag cgtgatcgcc tcgagcaagt
     2701 tctgggaagt gcaaaagcac atggcactga ccaaccacca gtacaacccg cagtccgtca
     2761 tcttcagcaa gaagatctgg gacaccctca gcgccgccga aaagaagatc atcagtgacg
     2821 cggcccacga agccacgaag ttccagcgcc agcagacgcg agcggctgtg ggcgtcaacc
     2881 tcgacctgct caagaaaagc ggcatgaacg tgaccgaact gcctccggcc gagatggcca
     2941 agttgcgcga aaaaatgaag ccggtgatcg cccagttcag tgcgagcgtc ggccagggaa
     3001 ccttgaacga ggtcacggcc gagctcgcga agatgcgcta gttggtggag cgaaagcgcc
     3061 gcgccgggag cccagaaacg ggggaagcgg ttcagccttg tcgtcgcgcc cgggcccgtg
     3121 ccagggccgc ctcgatcacc gcgcgcttgc gatcggcttg cgcatcctct gaatcgtgag
     3181 tttgcgccgg caggtccgcc agcttgttca gtgcctttgc tcccagccgg tcgcgctgct
     3241 ctttctcctc gcgctccagc cgctcctgcc ggaactggta gcgctcgagg gccagcgccg
     3301 cctgcgccgc cgaccaggct ttccaaccgg tgcgttcccc ggtgacgttc tccatgccga
     3361 tgcaatcgac cgggcagacc gggacgcaca gctcgcagcc cgtgcaatag cgctcgatca
     3421 ccgtatgcat cagcttgttg ctgcccacga tcgcatcggt gggacaagcg tcgaggcaaa
     3481 gcgtgcagcc tatgcaccag gcttcgtcga tgacagccac ggtgcgcggg ccctcggcgc
     3541 cgttgacggg gttcagcggg agaggcgggc gtccggtgat cgcggccagc cgcgcaatgc
     3601 cctgggcacc gccgggcggg cattgattga tttcggcctt gccgtccgct atggcctgcg
     3661 cgtagcccgc gcaatcggga taaccgcagc gggtgcactg ggtctggggc agggcggcgt
     3721 gcagccgcgc ggccagctcg ctcacctcag gcgattgacc gctgctggcc ggacttcctc
     3781 gcagccgccg gcccattggc gctcgagcgt ctgcccgcgt tgacgggcac cctcgggcct
     3841 tcgttgaact ggaggatgaa gtcgcgcacc tgcgggtaca cggcgtcgcg ccaacggcgg
     3901 ccgctgaaga tgccgtagtg accggcaccc ttgacttcca gatgcttctg gcgcgacttg
     3961 ggcacgttgt agcaaaggtc gtgggcggct tcggtctggc cggcgccgga gatgtcgtcc
     4021 agctcgccct cgatcgtgaa atgcgccgtc tcggtgatgt cctccggtcg cacgcgctcg
     4081 agatcgccgt cgtcgctgat cacgtcccag gtcccattga ccagggcgaa ctcctggaac
     4141 accacccgga tggtctccag gtagtagtcc gcgtccatgt cgagcacggc gttgtactca
     4201 tcgtagaacg tgcgatgcgc ctcggcgctc gcgtcgtcgc cggcgatcag gtgcttaaag
     4261 tagtcgtaat ggctcgacat gtggcggtcg gggttcatcg ccacgaagcc cgtgtgctgc
     4321 aggaaaccgg cgtagacacg ccgccccgcg cccgggaagc tggtcggcac ccggtagatc
     4381 acgttgttct cgaaccactc gtagctcttg ttcatcgcca ggttgttcac ggcggtgggc
     4441 gacttgcggg cgtcgatggg gccgcccatc atcgtcatcg tcatcggcgt gacctcgccg
     4501 cgcgaggcca taagcgacac cgcggccagc acgggaaccg tgggctggca gacgctgatc
     4561 acatggcaat ttccgtagat gccctgcagg tggcgtatga actcttccac gtaatgaacg
     4621 taatcgtcca ggtggaactc gccctgttcc agcggcaccg tgcgggcgtt cttccagtcg
     4681 gtgatgtaga ccttgtggtc cttgagcatg gtgcgcacgg tatcgcgcag cagcgtggcg
     4741 taatggccgg acagcggcgc cacgatcagc acggccggct ggcccttgag cttcgtcagc
     4801 gtggcggggt tgtcggtgaa gcgcttgaag cggcgcaact cgcagaaagg cttgtcgatt
     4861 tccacccgct cgtggatggc cacgtccgtg ccgtcgacgt tgacggtcct gatgccgaat
     4921 tgcggctttt catagtcctt ccacaaccgg tacatcaggt cgtagctggc cgacacgcgc
     4981 tgggcgaagg ggttctggcc gaatggcgat agcggattgc tgtacagctt ggaagctgcc
     5041 tgggcaaagt ccgcgaacgg ttccatcatg gaacgctggg cttcgtagat ctggtaaagc
     5101 atgggttcta atgtccatcc gttgttgcag tgcaatatat cacctggtaa caaacttggg
     5161 gtcgcgtaaa accaccataa gggaacggaa tttttcaggt cgtaagttcc acgtcacgaa
     5221 tgtcgcgcat tcacgc
//
LOCUS       DASWIY010000052         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_404363, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000052 DASWIY010000000
VERSION     DASWIY010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404363"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VGA59_02015"
     CDS             <1..1355
                     /locus_tag="VGA59_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX9718480.1"
                     /translation="EDPIEIQLGGINQVQVNIKAGLTFAATLRSILRQDPDVILVGEI
                     RDLETAEIAFHASMTGHLVFSTLHTNSTTATVARLLDLGVDPYLIGSSINLILAQRLM
                     RKICTKCRESYKPDPKLVDRLRLQEGEIPSYYHGAGCDACGQTGYSGRVGVFEMLRMT
                     PTVKELINRKASELELRKAALSAGTLLLLQDALDKIRQGITTIEELLRVIQIQEDEVV
                     RCPNCNSMINLDFSTCPYCLHSLRVVCGACGQELKGEWRICPYCNTKVTKMTLVGERE
                     HKAPFLLPGAVVESEAASDEGPRATAPPPPATADGQEAAAGTAPPLKAPRILVVDDDD
                     TIRRLVVKTLERMSSKPEVFTASDGGEGLMQVEKVKPDLVVLDIMMPGMSGFDVCQKL
                     RSNIKTAFIPILMLTGNTDEESRTQGFLVGTDDYMHKPFSVPELHARVSRLLRRTYGV
                     "
     gene            1377..>1855
                     /locus_tag="VGA59_02020"
     CDS             1377..>1855
                     /locus_tag="VGA59_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX9718481.1"
                     /translation="MIKDFYRLLGLPRGAPLDEIKSAYRRMAAQYHPDKVAGLGPEVQ
                     DVAQAKMLELNEAMALLADPAKRAEYDEVLDLIPERPMAPPAPSSSEPLPTGTEPGEP
                     KGGEAAPDADSESSYAMPKRASAAAPVSSVSRDLFLEQQGRELKVGLQGLTMQWRESA
                     "
BASE COUNT          352 a          587 c          611 g          305 t
ORIGIN      
        1 tggaggaccc gatcgagatc cagctgggcg gcatcaacca ggtgcaggtc aacatcaaag
       61 ccgggttgac ctttgcggcg acgctgcgct ccatcctgcg ccaggacccg gacgtcatcc
      121 tggtgggtga gatccgcgac ctggaaacgg ccgagatcgc cttccacgcc tccatgaccg
      181 gccacctggt gttttcaacc ctgcacacca actccaccac ggcgacagtg gcgcggctgc
      241 tggatttggg cgtcgacccc tacctgatcg gatcgtccat caacctcatc ttggcgcaac
      301 gcctgatgcg caagatctgc accaagtgcc gggagagcta caagcccgat cccaagctgg
      361 tggaccggct gcggcttcag gagggcgaga tcccgagcta ctaccacggg gccgggtgcg
      421 atgcctgcgg gcagaccggc tactcaggcc gcgttggcgt tttcgagatg ttgcggatga
      481 cgccgacggt gaaggagctg atcaaccgca aggcgtcgga gctggagctg cgcaaagcgg
      541 cgctttcggc gggcactctc ctgctgctcc aggacgcgct ggacaagatc cgccagggaa
      601 tcaccaccat cgaggaattg ctgcgcgtaa ttcaaattca ggaggacgaa gtcgtccgct
      661 gccccaattg caacagcatg atcaacctgg atttttccac ctgtccttac tgcttgcact
      721 cgctccgggt ggtttgcggc gcctgcgggc aggagctgaa gggcgagtgg cgcatctgcc
      781 cctactgcaa caccaaggtc accaagatga cgctggtggg cgagcgagag cacaaggcgc
      841 cgttcttgtt accgggagcc gtagtcgaga gcgaagcggc ttctgacgaa ggtccgcgtg
      901 ccaccgcccc gcccccgccc gccacggctg acggccagga ggcggcggcc ggcacagccc
      961 cgcctctgaa agctccgcgc atcctggtgg tggacgatga cgacaccatc cggcgcctgg
     1021 tggtgaagac cctggagcgg atgtcgtcca agcccgaggt cttcacggcc tctgacggcg
     1081 gcgagggctt gatgcaggtg gaaaaggtga agcccgacct ggtggtgctc gacatcatga
     1141 tgccgggcat gtcgggcttc gacgtctgcc agaagctgcg cagcaacatc aagacggcct
     1201 tcatccccat cctgatgctg acggggaaca ccgacgagga gagccgcacg cagggcttcc
     1261 tggtggggac cgacgactac atgcacaagc cgttttccgt gcccgagctg catgcgcgcg
     1321 tgtcgcggct gctgcgcagg acctacggcg tctgacggtc cgcggctgag ttcagcatga
     1381 tcaaagactt ctaccggctc ctcggactgc cccgcggggc gccgctggac gaaatcaagt
     1441 cagcctaccg tcgcatggcg gcgcaatacc accccgacaa agtcgcgggc ctggggcccg
     1501 aggtccagga cgtggcccaa gccaaaatgc tggagttgaa tgaggcgatg gccctcctgg
     1561 ctgacccggc caagcgtgcg gagtatgacg aagttctgga cttgatacct gaacggccaa
     1621 tggcgccgcc tgcgccttca agctcggagc cgttgccaac ggggacggaa cccggcgagc
     1681 caaagggtgg tgaggcggcg ccggacgccg attcggaatc gtcgtacgca atgcccaagc
     1741 gggcgtcggc ggcggcgccc gtctccagcg tgagccgcga tcttttcctc gaacagcagg
     1801 gccgcgagct gaaggtcggt ttgcagggat tgacgatgca gtggcgcgaa agcgc
//
LOCUS       DASWIY010000053         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1511432, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000053 DASWIY010000000
VERSION     DASWIY010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511432"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VGA59_02025"
     CDS             <1..260
                     /locus_tag="VGA59_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX9718482.1"
                     /translation="LTRELFEGAIEPLLERVRASVTQLLGDAGVGVAQVDTLFFTGGS
                     SGIPALRQSVAAMLPNARQVEGNTFGSIGSGLAIEAQKRYG"
     gene            complement(432..2765)
                     /locus_tag="VGA59_02030"
     CDS             complement(432..2765)
                     /locus_tag="VGA59_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HEX9718483.1"
                     /translation="MHAAKPLFDYPKYWAECFGPAPFLPMSREEMDQLGWDSCDIIIV
                     TGDAYVDHPSFGMAIIGRLLESQGFRVGIIAQPDWSSKDDFMKLGEPNLFFGVAAGNM
                     DSMINRYTADKKIRSDDAYTPGGLAGKRPDRASLVYSQRCKEAYKHVPIVLGGIEASL
                     RRIAHYDYWQDKVRHSILIDACADILLYGNAERAIVEVAQRLSYGHKIEEISDIRGTA
                     FIRRDTPAGWMEIDSSRIDRPGKVDKIINPYVNTQDTQACAIEQEKGPIEDPNEAKVV
                     QLLAHPRLERDKTVIRLPSFEKVRADSVLYAHANRVLHLETNPGNARALVQKHGEVDV
                     WFNPPPIPMTTEEMDYVFGMPYTRVPHPAYGGAKIPAYEMIRFSINIMRGCFGGCTFC
                     SITEHEGRIIQNRSQESIIREIEEIRDKVPGFTGVISDLGGPTANMYRIACKSPEIES
                     ACRKPSCVFPGICENLNTDHSALIQLYRSARALPGVKKILIASGLRYDLAVESPEYVK
                     ELVTHHVGGYLKIAPEHTEEGPLNKMMKPGIGSYDKFKRMFEKYSKEAGKEQYLIPYF
                     IAAHPGTSDEDMMNLALWLKSSGFRADQVQAFYPSPMATATAMYHSGKNPLRKVTYKS
                     DGVPIVKSEAQRRLHKAFLRYHDPKGWPMLREALERMGRSDLIGPGKHQLIPLHQPAS
                     DSYQSARRKNSTPAGSKKVGGAAAARSGGDKTRPLLTQHTGLPPRDSGAGAGNPWDKR
                     EQAKAAAQARNQQAAKERADAAKGKGRKPPRKPVAPR"
BASE COUNT          430 a          859 c          959 g          557 t
ORIGIN      
        1 agctgacccg cgaattgttc gagggcgcca tcgagccgct gctcgagcgt gtgcgcgcca
       61 gcgtcaccca gttgctcggc gatgccgggg tgggcgtggc gcaggtcgat acgctgttct
      121 ttaccggcgg ctccagcggc atcccggcct tgcgccagag cgtggcggcg atgctgccca
      181 acgcccgcca ggtggaaggc aataccttcg gcagcatcgg cagcggcctg gccatcgagg
      241 cgcagaagcg ttacggctga tagcgtcgcc tgtagcccgg ataagcgcag cgcaatccgg
      301 gagcgattca ggccatccac cctgcccggc taaacagcgc aaaagctcgc ggctaaagcc
      361 cctcccacgg gatttacagt gtccgtggga ggggctttag ccgcgacagg ctgcgaccgc
      421 ttacaagcca atcagcgcgg tgctacgggc ttccttggcg gcttcctgcc tttgcccttg
      481 gcggcatccg cgcgttcctt ggcggcctgc tggttgcgcg cctgggccgc ggccttggct
      541 tgctcgcgct tgtcccaggg gttgcccgcg cccgcgccgc tgtcgcgcgg cggcagaccg
      601 gtgtgctggg tcagcagcgg ccgggtcttg tcgccgccac tgcgcgctgc ggcggcaccg
      661 ccgaccttct tgctgccggc cggggtggag ttcttgcgcc gcgcgctctg gtagctgtcg
      721 ctggccggct gatgcagcgg gatcaactgg tgcttgcccg gcccgatcag gtcgctacgg
      781 cccatgcgtt ccagcgcctc gcgcagcatc ggccagccct tgggatcgtg gtaacgcagg
      841 aaggccttgt gcaggcgacg ctgcgcctcg ctcttgacga taggcacgcc gtcgctcttg
      901 taggtgacct tgcgcagcgg gttcttgccc gagtggtaca tggcggtggc ggtggccatc
      961 ggcgacgggt agaaggcctg cacctggtcg gcgcgaaagc cgctgctttt cagccacagg
     1021 gcgaggttca tcatgtcctc gtcactggtg ccggggtggg cggcgatgaa gtaggggatc
     1081 aggtactgct ctttgcccgc ctctttcgag tacttctcga acatgcgctt gaacttgtcg
     1141 tagctgccga tgcccggctt catcatcttg ttcagcgggc cttcttcggt gtgctccggg
     1201 gcgatcttca ggtagccgcc gacatggtgg gtgaccagtt ccttgacgta ctccggcgac
     1261 tccacggcga ggtcgtagcg caaccccgag gcgatcagga tcttcttcac ccccggcagc
     1321 gcgcgggccg agcggtacag ctggatcagc gccgagtggt cggtgttgag gttctcgcag
     1381 atgccgggga acacgcagga cggcttgcgg caggcggact cgatctccgg gctcttgcag
     1441 gcgatgcggt acatattggc ggtcggcccg ccgaggtcgg aaatcacccc ggtgaaaccc
     1501 ggcaccttgt cgcggatttc ttcgatctcg cggatgatcg actcttgcga gcggttctgg
     1561 atgatccggc cttcgtgctc ggtgatcgaa cagaaggtac agccgccgaa gcagccacgc
     1621 atgatattga tcgagaagcg gatcatctcg taggccggga tcttcgcccc gccataggcc
     1681 gggtgcggca cccgcgtata gggcatgccg aacacgtagt ccatctcttc ggtggtcatg
     1741 gggatgggtg gcgggttgaa ccagacatcc acctcgccgt gcttctgcac cagggcgcgg
     1801 gcgttgcccg ggttggtttc caggtgcagc acgcggttgg cgtgggcgta gagcaccgaa
     1861 tcggcgcgga ccttctcgaa cgagggcagg cggattacgg tcttgtcgcg ctccaggcgc
     1921 ggatgcgcca gcagctgcac caccttggcc tcgttggggt cctcgatcgg ccccttttcc
     1981 tgctcgatgg cgcaggcctg ggtgtcctgg gtgttgacgt aggggttgat gatcttgtcg
     2041 accttgcccg gccggtcgat gcggctggag tcgatctcca tccagccggc gggcgtgtcg
     2101 cggcgaatga aggcggtgcc gcggatatcg ctgatctctt cgatcttgtg gccgtaggac
     2161 aggcgctggg ccacttcgac gatggcccgc tcggcgttgc cgtagagcag gatatcggcg
     2221 caggcatcga tcaggatcga gtggcgcacc ttgtcctgcc agtaatcgta gtgggcgata
     2281 cgccgcagcg aggcttcgat gccgccgagc acgatcggca cgtgcttgta ggcttccttg
     2341 caacgctggc tgtagaccag gctggcgcga tccgggcgct tgccggccag accgccaggc
     2401 gtgtaggcat cgtcggaacg gattttcttg tcggcggtgt aacggttgat catcgaatcc
     2461 atgttgccgg ccgcgacgcc gaagaacaga ttgggctcgc cgagcttcat gaaatcgtct
     2521 ttgctgctcc agtccggctg ggcgatgatg cccacgcgaa agccctggga ctcgagcagc
     2581 cggccgatga tcgccatgcc gaacgacgga tggtcgacat aggcatcgcc ggtgacgatg
     2641 atgatgtcgc agctgtccca gccgagctga tccatctctt cccggctcat cggcaggaaa
     2701 ggcgccgggc cgaagcactc agcccagtat tttggatagt cgaataacgg cttggctgcg
     2761 tgcatgaaaa tgaccgagat gtcaggatta aacggtcgcg gaata
//
LOCUS       DASWIY010000054         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2997561, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000054 DASWIY010000000
VERSION     DASWIY010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2997561"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VGA59_02035"
     CDS             <1..343
                     /locus_tag="VGA59_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718484.1"
                     /translation="ETWKRLPEFATLELDIIQVKGKKIGLHVYALMGDPELAKRPDFV
                     KAKETVDTMLAAYRSQQWDKAEALIKELRVIGEPFHLDYLCELYEGRIAAYRIDPPPA
                     DWDGVFIATSK"
     gene            complement(381..1385)
                     /locus_tag="VGA59_02040"
     CDS             complement(381..1385)
                     /locus_tag="VGA59_02040"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEX9718485.1"
                     /translation="MGTYYDQLNESEREVIGRLHSAGRSIREIARTLARSASTISREI
                     RRNSRPTKRWYGGYVAARAQVLCERRRNRGRTHKLARQPDLRNLVRDSLAMGWSPEQI
                     AGRLALEHGRTIVSHESIYRYVYHRSSQKDYWHRLLPRAKHRRGRLGRRGGSPVDHIR
                     ERVPLSARPAEAADRRQPGYWEADLMLFRRYGQAVLVTHERASRFTLITRQPNKAAQP
                     VLDSLLARFTRLPAKLRRSITFDNSTEFAFHHRLKATLGLQTFFCDPHAPWQKGGIEN
                     AIGRLRRPLPRKSNLATISQEDLDELTTQYNNTPRQCLGFRTPAEVFSSFLNCVALET
                     "
     gene            complement(1523..>1726)
                     /locus_tag="VGA59_02045"
     CDS             complement(1523..>1726)
                     /locus_tag="VGA59_02045"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808490.1"
                     /note="NADPH-dependent; catalyzes the reduction of
                     7-cyano-7-deazaguanine to 7-aminomethyl-7-deazaguanine in
                     queuosine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEX9718486.1"
                     /translation="NIGTFHEDCTLRIAKDLIKVLQPRWLRIAGYWYPRGGMPIDVFY
                     QSAPPPEGLWLPDQGVAPYRGRG"
BASE COUNT          297 a          511 c          592 g          326 t
ORIGIN      
        1 cgagacgtgg aagcgcctgc cggaattcgc cacgctggag ctcgacatca tccaggtgaa
       61 gggcaagaag atcgggttgc acgtctacgc attgatgggc gatccggagc tcgccaagcg
      121 gcccgacttc gtcaaggcca aggagaccgt ggacaccatg ctcgcggcct atcgcagcca
      181 gcagtgggac aaggccgagg cgctcatcaa ggagctccgc gtcatcggcg agcccttcca
      241 cctcgattac ctgtgcgagc tctacgaggg ccggatcgcc gcctaccgca tcgatccgcc
      301 gcccgccgac tgggacggcg tgttcatcgc gaccagcaag tagattcagc cgtcattccc
      361 gcgcaagcgg gaagatggtc tcacgtttca agtgcaacac agttgaggaa gcttgagaag
      421 acctctgccg gggtcctgaa gccgagacat tgtcgtggcg tgttgttgta ctgggtggtg
      481 agctcatcga ggtcctcctg agagattgtt gcgaggttgg attttctggg cagcggccga
      541 cgcaggcggc cgatggcatt ttcgatgccg cctttctgcc atggcgcatg gggatcgcag
      601 aagaaggtct gcaggccgag ggtggccttc agccggtggt ggaatgcgaa ctcggtgctg
      661 ttgtcgaaag tgatggagcg acggagcttg gccggtaatc tggtgaagcg ggcaagcaga
      721 ctgtcgagca ccggctgtgc cgccttgttg ggctggcggg tgatcagagt gaagcgggag
      781 gcccgctcat gggtgacgag cacggcctgg ccgtagcggc ggaacagcat gaggtcggct
      841 tcccaatagc cgggctggcg gcgatcggcg gcttccgcgg gcctggcgct gagcggcacg
      901 cgctcgcgga tatgatcgac cgggctgccg ccacggcgtc cgagccggcc gcggcgatgc
      961 ttggcgcgcg gcagcagccg gtgccagtaa tccttctggc tgctgcggtg atagacatag
     1021 cggtagatgg actcgtggct gacgatcgtg cgaccgtgct caagcgccag ccggccggcg
     1081 atctgttcag gcgaccatcc catcgcaagg ctgtcgcgga ccagattgcg caggtccggt
     1141 tggcgcgcca gcttgtgggt tcggccgcgg ttgcgtcttc gctcgcacag aacctgggca
     1201 cgtgccgcca cgtagccgcc gtaccagcgc ttggtgggac ggctgttgcg ccggatctcc
     1261 cggctgattg tgctggccga tcgcgccaaa gtccgtgcga tctcacgaat ggaccggccg
     1321 gctgaatgca gacgcccgat cacctctcgc tcactctcgt tgagctggtc ataataggtt
     1381 cccatcgcaa caccagttta taggtgttgc acttgctccg tgattctggc gggtccaggg
     1441 gcaagcgacg tgccgatggc tctggattcc cgcttgcgcg ggaatgacga cgcgtgaatg
     1501 ttcgttgact ttatccctga ctttatcccc ggccgcgata gggcgccacc ccttgatcgg
     1561 gcagccacag cccttcgggc ggcggggcgg actggtagaa cacgtcgatc ggcatgccgc
     1621 cgcgcggata ccaatagccg gcaatgcgca gccagcgcgg ctgcaggacc ttgatcagat
     1681 ccttggcgat gcgcagcgtg caatcctcgt ggaaggtgcc gatatt
//
LOCUS       DASWIY010000055         4382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1512192, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000055 DASWIY010000000
VERSION     DASWIY010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512192"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VGA59_02050"
     CDS             complement(<1..1207)
                     /locus_tag="VGA59_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX9718487.1"
                     /translation="MKPPTKTPVSDDLAQLVGQSVNAIDTPALVVDLDAMKRNLGRMA
                     EFARKHDVRWRPHAKMHKSVALAKLQMLAGAVGVCVQKTAEAEIMAAGGVHDLYITNE
                     VVAAPKLARVAALAHRVTAENGQLAIAVDSVEGVGRLAQAMNETRSNRGAAAVIAAVI
                     DVFVEIDVGQGRCGVPPGQAAVALVHEIRKHPALRFAGLQAYHGKAQHLRSAQQRREA
                     IAIAVDDVVYTRKLIEAEGVPVDLVTGAGTGSMVCEAASGVFGELQAGSFLFMDADYA
                     DNERDAAQPQFEHALFVKTQVVSSRDDHAVCDAGHKSHAIDSGLPKVHVFDEESELDY
                     FNGGDEHGILRPAGGSRRVPAIGRMLWLVPGHCDPTVNLYDFMIGVAGGLRRGTVERI
                     IRVDARGAAT"
     gene            complement(1176..1820)
                     /locus_tag="VGA59_02055"
     CDS             complement(1176..1820)
                     /locus_tag="VGA59_02055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9718488.1"
                     /translation="MEATRIIVVRHGETAWNVDARIQGQLDVGLNETGRWQARRVGEA
                     LAGEAITAVYSSDLGRAHQTAQTIAEVTGTPVVAEEGLRERSFGMFEGKTFTEIHETW
                     PDHAQNWRKRIPEWAPPEGGESLLQLRERVTRTMQSLAARHRGEQIVVVAHGGVLDTL
                     YRVATGQDVNSPRTWELPNGAINRLLWTPEGFTLVGWSDTQHLDDEAADENTSF"
     gene            complement(1876..2484)
                     /locus_tag="VGA59_02060"
     CDS             complement(1876..2484)
                     /locus_tag="VGA59_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718489.1"
                     /translation="MRILRKNPSILRAAGWMVLALVLLSGCATSYLLDNDVQSFSGLT
                     ALPAQPSYRFERLPSQQSPAQAQLEAMADPALHKAGLRRDDANPHYSIQVSARLRRVL
                     SPWASPWDGWGGWGWGAGFGHSHGFGHMGLFGRMESPWYQREVDVIVRELPSNRVVFE
                     SHAANDGPWLDNTAVLPAMFEAALHGFPNPPAGPRRVNIQIG"
     gene            2573..3349
                     /gene="ttcA"
                     /locus_tag="VGA59_02065"
                     /pseudo
     CDS             2573..3349
                     /gene="ttcA"
                     /locus_tag="VGA59_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461304.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
     gene            complement(3578..4336)
                     /locus_tag="VGA59_02070"
     CDS             complement(3578..4336)
                     /locus_tag="VGA59_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9718490.1"
                     /translation="MPYVTLHGDGPVSIVTLNRPDRLNAIGTALLGDLQTALTQAQTD
                     PGTRAIVLTGNGRAFCAGDDLKEFGEQSESPESIAIYADRIQQITRTIMFGDKLVIGA
                     VHGYAVGGGFEWVLNCDMVVAADDLVCFFPEMSLGHFVTGGVTHLLPLAVGYQKAMEL
                     IVLGERQKATDLLRLGIVNEVVPAQQTLARALELANKVAERSPYALGRLKRVMTSEIS
                     SQLSGALDLERRACAALFAESDTAARIEKFAKEL"
BASE COUNT          738 a         1461 c         1402 g          781 t
ORIGIN      
        1 aggtcgcggc gcctctcgcg tcaaccctga tgatgcgctc caccgttccc ctgcgcaggc
       61 cgccggcgac tccgatcatg aagtcgtaga ggttcaccgt cgggtcgcag tggccgggga
      121 cgagccacag catccgcccg atggcgggca cccggcggct gcctcctgcc ggccgcagga
      181 tgccatgctc gtccccgccg ttgaagtaat ccagctcgct ttcctcatcg aagacgtgga
      241 ccttgggcaa gccggagtcg atcgcatggc tcttgtggcc ggcatcgcac acggcgtgat
      301 cgtcgcgcga gctgacgacc tgggtcttga cgaacagcgc gtgctcgaac tgcggttgtg
      361 ccgcatcgcg ctcgttgtcc gcgtagtcgg cgtccatgaa caggaacgac cccgcctgca
      421 gctcgccgaa caccccactg gcggcttcgc acaccatgct gccggtgccc gccccggtga
      481 ccaggtcgac cggcacgcct tcggcttcga tcagcttgcg cgtatagacc acgtcgtcca
      541 ccgcgatggc gatcgcctcg cgccgttgct gcgcgctgcg caggtgctgg gccttgccgt
      601 gataggcctg caagccggcg aaccgcagtg ccgggtgctt gcggatctcg tgcaccaggg
      661 ccacggccgc ttgcccgggg ggcacgccgc atcggccctg gccgacgtcg atttcgacga
      721 agacgtcgat cacggcagcg atcacggcag cggcgccccg attgctgcgc gtttcgttca
      781 tcgcctgcgc cagccggccc acaccctcca cgctgtccac cgcaatcgcc agctggccgt
      841 tttcggcggt gacccggtgc gccagcgcgg ccacccgtgc cagtttgggg gcggcgacga
      901 cctcgttggt gatgtaaagg tcgtggacgc cgccggccgc catgatttcg gcttcggccg
      961 tcttctgcac gcacacgccc acggcgccgg ccagcatctg gagcttggcc agcgccacgc
     1021 tcttgtgcat cttggcgtgg ggccgccagc ggacgtcgtg tttcctggcg aactcggcca
     1081 tgcggcccag gttgcgcttc atcgcgtcca ggtcgacgac cagggcgggt gtgtctatgg
     1141 cgttgacact ctggccgacc agttgggcca ggtcatcaga aactggtgtt ttcgtcggcg
     1201 gcttcatcgt ccagatgttg agtatcggac cagcctacca gagtaaagcc ttccggcgtc
     1261 cacagcagcc gattgatggc gccgttgggc aactcccagg tccggggcga attgacatcc
     1321 tgcccggtcg cgacccggta caaggtgtcc agcacgccgc cgtgggccac caccacgatc
     1381 tgctcgcctc gatggcgcgc cgccagcgat tgcatggtgc gcgtgacgcg ctcgcgcagc
     1441 tggagcagcg actcgccgcc ttcgggcggc gcccattcgg gaatgcgttt gcgccagttc
     1501 tgcgcgtggt cgggccaggt ctcgtggatc tcggtgaaag tcttgccctc gaacatcccg
     1561 aagctgcgct cgcgcaggcc ttcttcggcc accacgggcg tgccggtgac ttcggcgatc
     1621 gtctgcgccg tctggtgggc gcggccgaga tcgctcgagt aaacggcggt aatcgcttcg
     1681 ccggccagcg cttcgccgac ccgccgcgcc tgccatcggc cggtctcgtt caagccgacg
     1741 tccaactggc cctggatgcg cgcatccaca ttccaggcgg tctcgccatg gcgcacgaca
     1801 atgattcgcg tagcttccat ctacgcgcat tttattgggg gcttggagca atttctctta
     1861 aagcgtagcc agttgttatc cgatctggat attcacccgg cgcggcccgg ccggcgggtt
     1921 ggggaaaccg tgcagagcag cttcgaacat ggccggcaac accgccgtat tgtcgagcca
     1981 gggcccgtca ttggcggcgt ggctctcgaa aacgacgcga ttggacggca actcgcgcac
     2041 gatgacatcg acttcccgct ggtaccacgg ggattccatg cgcccgaaaa gacccatgtg
     2101 gccgaatccg tgactgtggc cgaacccggc gccccagccc cagccgcccc agccgtccca
     2161 gggactggcc cagggcgaca gcacccgccg cagccgggcg gacacctgga tgctgtagtg
     2221 gggattggca tcgtcacggc gcagcccggc cttgtgcagg gcggggtcgg ccatggcttc
     2281 cagctgcgcc tgggccggcg attgctgcga aggcagccgc tcgaagcggt agctgggttg
     2341 tgccggcaag gcagtgaggc cggagaacga ctggacatcg ttgtccagga ggtaactggt
     2401 ggcgcagccg gacaacagca cgagggccag caccatccag cccgccgcgc gcagtattga
     2461 tgggtttttg cgcaatatcc gcatgcaggt gagaccctcg aggccgtcct gtgttccagg
     2521 caatcctaga attgcaaacc ccgtcgcaaa agctgtccgc atgaatccaa ccgattacaa
     2581 caagctccgc aagcgcatcc gccgggaggc cggccgggcc gtcggcgact tcgagatgat
     2641 cgccgatggc gacctcgtga tggcctgcct ctccggaggc gccgacagct acaccatgct
     2701 cgacaccttg ctctacctca aggaggtggc gcccatcaag ttcgacgtga tcgccgtcaa
     2761 cctggaccag aagcagcccg gatttcctga acatgtcctg ccgcggtacc tcgcctcagt
     2821 gggcgtgcct ttcaggattg tcgagcagga cacctactcc gtcgtcaagg agaagatccc
     2881 cgacggcaag accacctgtt ccttgtgctc gaggttgcgg cgcggggtcc tgtaccggac
     2941 ggcgcgggaa atcggtgcga acaagatcgc actgggccat caccgggagg acatcctcga
     3001 gaccctgctg ctgaacatgt tctacggcgg cacgctgaag gcgatggcgc cgaagctgct
     3061 gtccgacgac ggccacaacg tggtcatcag gccgctggtg tactgcaagg agaaagacat
     3121 caaggcgtac gccgggcaaa tggcttttcc gatcattccg tgcgacctgt gcggctcgca
     3181 accgaatcta cagcgcaagg ccatgaaaga tctgctcgcc acctgggaca agcagttccc
     3241 gggccgagtg gacaacatgt tgcgggcgct ggcgaacgtg atcccttccc acttgcttga
     3301 ccggaaactg gccggcttcg gctcggcccg gctccagccg ggcgaagagc ccatggccga
     3361 atggctttcg gcgcaacctg agggtcgcct gcaacagccc atcgcgctgc aatgggctga
     3421 tctggcagag gatgttttgt tgcgcagcta ggagaggagc cgggagagag accttgcccc
     3481 agggacggga cgcgcagccg gaactgcgct ggcatccgct gcggggcgag ggacccccac
     3541 gggcggcaaa gccgcgtttt cgcctgaaga cgctgaccta caactccttt gcgaacttct
     3601 cgatgcgcgc cgctgtgtcc gactccgcaa acagcgcggc acacgcgcgc cgttcaaggt
     3661 cgagtgcccc agagagctgg ctactgatct cgctggtcat gacgcgcttg aggcgaccca
     3721 aggcatacgg cgagcgctcc gcgaccttgt tggcgagttc cagtgcccgc gccagcgtct
     3781 gctgcgctgg gacaacttca ttcacgattc ccaggcggag caggtccgtc gccttctgcc
     3841 gctcgccaag gacgatgagc tccatcgcct tttgataccc gacggcaagc ggcagcaggt
     3901 gggtgacacc gccggtcacg aagtgcccaa gggacatctc ggggaagaag cacaccagat
     3961 cgtccgcagc caccaccatg tcgcagttca agacccactc gaagccgccg cctacggcat
     4021 atccatgcac ggcaccgatg accagcttgt cgccgaacat gatcgtgcgg gtgatttgct
     4081 ggatgcggtc tgcatagatc gctatcgact cggggctttc cgactgctcg ccgaactcct
     4141 tgaggtcgtc gcccgcgcag aatgcgcgac cattgccggt gagaacgatc gcgcgcgtcc
     4201 cggggtcggt ctgcgcctgg gtgagggccg tctgaaggtc gccgagcagg gcggtgccga
     4261 tcgcattcag ccggtcgggc cggttcaagg tcacgatgga cacgggccca tcgccgtgga
     4321 gcgtcacata tggcattgtc atctctcctc tatcgtttcg cactcatctt ttcgcggtcc
     4381 ca
//
LOCUS       DASWIY010000056         4304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3358895, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000056 DASWIY010000000
VERSION     DASWIY010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3358895"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1045)
                     /locus_tag="VGA59_02075"
     CDS             complement(68..1045)
                     /locus_tag="VGA59_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9718491.1"
                     /translation="MITSLRSVLARFGAVVAVTALAASAALAAFPEKPVKLVVPFSPG
                     GGTDAIARTLGVGMAQALGQPVIVDNKPGAGTIIGTDFVAKSPADGYTLVVATFAHAV
                     NPTLMPKLPFDTDKAFDPVILLARGPNVLVVRADSPYKSVADVLAAARATPGKLTFAS
                     QGNGTSAHLAGEMFGNLAKVKTTHIPYKGAGPAMTDLLGGQVDMMFATAAAATPQIAS
                     GKLRALGVTTPKRSPAMKDVPAIAETVPGYAVESWYGLFAPAGTPADVIAKLHAAAKK
                     ATDSADFRKKIEPEGLTVSTGAPAELDTYVRAEQARWGKVVKESNLKID"
     gene            1211..2035
                     /locus_tag="VGA59_02080"
     CDS             1211..2035
                     /locus_tag="VGA59_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9718492.1"
                     /translation="MSASSDPRPAYSAAKTLASEKPGRSPANRSLERGIEILRSFRPG
                     SELLGNAELAERTGLSRSTVSRLSQTLVGTGFLQIDPRSRAYRLAPAVLSLAHAMRTG
                     STALAIAAPRMRAAAEAHRINVGLAAPDRDEMVYLESIRYNRRPSLRTVVSGQRVPME
                     LTSLGRAYLASATEAQRKTLLAHFRRVRRAQWAELGPQLMAAIRSVEEKGYCAASWQA
                     EVVAVATPLAFQGSRYALNASLSTSEPVAAAVRELAPTLLALKALIQRDLDAAQEA"
     gene            complement(2115..2330)
                     /locus_tag="VGA59_02085"
     CDS             complement(2115..2330)
                     /locus_tag="VGA59_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718493.1"
                     /translation="MLNCKEVTELCSQEMERPLRLGERLSLGTHLMMCTGCTNFRRQM
                     KALRLVAHTYAEGGAAAAPPADERRAK"
     gene            complement(2324..2953)
                     /locus_tag="VGA59_02090"
     CDS             complement(2324..2953)
                     /locus_tag="VGA59_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9718494.1"
                     /translation="MSRLPVASPGPADTEGPGISAEALIALRTDMFRFAQLQLRNRET
                     AEDMVQEAIESALRNAASFAGHSSLKTWVFAILKNRIIDHLRQAGRTVPISSLVEDGE
                     DWQERLETLFNERGGWRDSARPTAWPNPEESMQNKQFWVVFEACLDHLPANTGRVFMM
                     REFLGLESDEICAQLSISTSNCHVILHRARLKLRACLGNGWARMGDATC"
     gene            3135..4133
                     /gene="arsS"
                     /locus_tag="VGA59_02095"
     CDS             3135..4133
                     /gene="arsS"
                     /locus_tag="VGA59_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463910.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEX9718495.1"
                     /translation="MHATLPLLVRTDFPVLHRRVVDTLQVNLGYRCNQSCVHCHVNAG
                     PNRTEEMSGGTVDAVIAFLEASPRVRTLDLTGGAPELNRHFRRLVIAARALGVRVIDR
                     CNLTILEEPGQEGLAEFLAAHGVEIVASMPCYLEDNVDRQRGKGTFNASIRGLQRLNA
                     LGYATAGMGLALNLVYNPQGASLPPPQEALERDYKQHLAERFGVVFNQLFTLANMPIQ
                     RFGSMLVSKGQFNSYMSLLRGAHREDNLEQVMCRSLVSVDWLGYLYDCDFNQQLGLPI
                     RDAGKPRVHLAEITASALEGQPIRVANHCFGCTAGQGSSCGGALEAATASAPGCGI"
BASE COUNT          683 a         1467 c         1407 g          747 t
ORIGIN      
        1 atgccgctct cgacttgaag ctcgatcagc gagtagttca tggtgctgtc cgtggccgcg
       61 tcggccttca gtcgatcttg agattgcttt ccttgacgac cttgccccag cgggcctgtt
      121 cggcccggac gtaggtgtcc agttccgccg gagcgccggt gctgaccgtg aggccttccg
      181 gctcgatctt cttgcggaaa tccgcgctgt ccgtggcctt cttcgcagcg gcatggagct
      241 tcgcgatgac gtccgccggc gtgcccgcgg gagcgaacag gccataccag ctttccaccg
      301 cgtagcccgg gacggtttcg gcgatggccg gcacatcctt catcgcgggc gatcgcttcg
      361 gggtggtgac acccagcgcg cgcagcttgc cgctcgcgat ctgcggcgtg gcggccgccg
      421 cggtggcgaa catcatgtcc acctggccac cgagcaggtc ggtcatggcc ggacccgcgc
      481 ccttgtacgg gatgtgggtc gtcttcacct tcgccaggtt gccgaacatc tcgccggcga
      541 ggtgcgccga ggtcccgttc ccctgcgagg cgaaggtcag cttgccaggc gtcgcccggg
      601 ccgcagccaa gacgtccgca accgacttgt aggggctgtc tgcgcgcacg accagcacgt
      661 tgggcccgcg ggccagcagg atcaccggat cgaacgcctt gtcggtgtcg aacggcagct
      721 tcggcatcag ggtgggattc acggcgtggg caaaggtcgc cacgaccagg gtgtagccgt
      781 cggccgggct cttggcgacg aagtcggtgc cgatgatggt tcccgcaccg ggcttgttgt
      841 cgacgatcac gggctggccg agcgcctgcg ccatgcccac gcccagggta cgggcgatcg
      901 catcggtgcc gccaccgggc gagaagggga cgaccagctt caccggcttc tctggaaatg
      961 cggcaagcgc ggcggatgcg gccaaagcgg tgaccgcgac gacggcgccg aagcgcgcga
     1021 ggacggaacg gagggaagtg atcatggcgt tgtctcctga ctgcatggca atgtctcgtg
     1081 gggccggcgg tggcaacacg caccgccatc ggaatgcaaa tcttaggcat tgccgaccgg
     1141 attggcaccc caccaattcc aaccagtgga attcgataca ctgggcccca cctgtgtctc
     1201 aggacgtgca atgagtgctt cttcggatcc gaggcctgcg tactcagcag ccaaaacgct
     1261 cgcgagcgag aagccggggc gttcccccgc gaaccggtcc ctggagcggg gcatcgagat
     1321 cctgcgctcg ttccgccccg gctcggagct gctgggcaac gcggagctcg ccgagaggac
     1381 cggcctttcc cggtccaccg tcagccggct gagccagacg ctggtcggca ccggcttcct
     1441 gcagatcgac ccgcgcagcc gcgcatatcg gctcgctccc gcggtcctca gcctggccca
     1501 tgccatgcgc accggctcca cggccctcgc gattgcggcg ccgcgcatgc gcgccgccgc
     1561 cgaggcccac cggatcaacg tgggcctggc cgcacccgac cgggacgaga tggtttacct
     1621 ggagtccatc cgctacaacc gccgcccttc gctgcgcacg gtggtgtcgg ggcagcgggt
     1681 gccgatggaa ctgacttcgc tcggacgcgc gtacctggcg agcgcgaccg aggcgcagcg
     1741 caagacgctg ctcgcccact tccggcgcgt ccgccgcgcc cagtgggccg agctggggcc
     1801 gcagctcatg gcggcgatcc gcagcgtcga ggagaaaggc tactgcgcgg cctcctggca
     1861 ggccgaggtc gttgccgtgg cgaccccgct ggcattccag ggatcgcgct acgccttgaa
     1921 cgccagcttg tccacgtccg agccggtcgc ggcagcggtg cgtgagctcg cgcccacgct
     1981 gctcgcgctg aaggccctca ttcagcgcga cctggatgcc gcacaggagg cttgatacga
     2041 ccctgctgaa cgcacgatct ctcccacatt ggcgttcgtg gcacccgcga atgaccggaa
     2101 cgcccgataa gtcgtcattt cgcgcggcgc tcgtctgccg gtggcgccgc ggcggcgccg
     2161 ccctcggcgt aggtgtgcgc aaccaaccgc agcgccttca tctgcctgcg gaaattggtg
     2221 cacccggtgc acatcatcag gtgcgtcccc agcgacaagc gttcgcccag gcgcaatggc
     2281 cgctccattt cctgcgagca aagctcggtg acttccttgc agttcaacat gtcgcgtccc
     2341 ccatgcgagc ccaaccgttt ccaaggcaag cccgaagttt cagccgggcc ctgtgcagga
     2401 tgacgtggca attgctggtc gagatggaca actgagcgca gatttcgtcg gactccaggc
     2461 ccaggaactc gcgcatcatg aacacccgcc cggtgttggc cggcagatgg tcgaggcaag
     2521 cctcgaacac cacccagaat tgtttgtttt gcatggattc ctcaggattc ggccaggcgg
     2581 tggggcgcgc gctgtcccgc cagcctcccc gctcattgaa cagtgtttcg aggcgctcct
     2641 gccaatcctc cccgtcttcg accaggctcg atatcggcac cgtgcgcccg gcctgacgga
     2701 ggtggtcgat gatgcggttt ttcaggatgg cgaacaccca ggtcttgagc gaggagtgtc
     2761 ccgcgaaaga ggccgcgttg cgcagcgccg actcgatcgc ctcctggacc atgtcctcgg
     2821 cggtttcgcg gttgcgcaac tgcagttgcg caaacctgaa catgtccgtc cgaagcgcga
     2881 tcagcgcctc ggcgctgatg cctgggccct cggtgtcagc gggcccaggc gaagcgacgg
     2941 gcagcctcga cattcggtct cctcgataat tggttgactg tcgttgcgcg ctgcgcggcg
     3001 acaggaatgg ctggacgaaa ttctgctcca tgtcccttgg acgggcgccc ccccggtttc
     3061 ttacacgggt caaggcggcg tgtaaggaat cggtcggcgg cacgtccaac cagccatcgc
     3121 accaaggatc cgccatgcat gccaccctgc ccttgctcgt ccgaacggac tttcccgtcc
     3181 tgcaccgccg tgtcgtcgac accctccagg tcaaccttgg ctaccgctgc aaccaaagct
     3241 gcgtgcactg ccatgtcaac gccggcccca accgcaccga ggagatgtcg ggcggcaccg
     3301 tcgatgcggt catcgccttc ctcgaggcca gtccccgggt ccggaccctg gatctgacgg
     3361 ggggcgcgcc cgagctgaac cggcatttcc ggcggctggt catcgcggcg cgcgcgctgg
     3421 gcgtgcgggt gatcgaccgc tgcaatctca ccatcctgga ggagccgggc caggaagggc
     3481 tggcggagtt cctggccgca cacggggtgg agatcgtggc ctcgatgccc tgttatctgg
     3541 aagacaacgt cgaccggcaa cgtggcaagg gcaccttcaa cgccagcatc agggggcttc
     3601 agcggctcaa cgcccttgga tacgcaacgg ccggcatggg actggcgctc aatctggtct
     3661 acaaccccca gggcgccagt ctgcccccgc cccaggaggc gctggagcgg gactacaagc
     3721 agcaccttgc cgagcgcttc ggtgttgtct tcaaccagtt gttcacgctt gccaacatgc
     3781 cgatccagcg ctttggatcg atgctggtct ccaagggcca attcaacagc tacatgtccc
     3841 tgctgcgcgg cgcgcaccgg gaagacaacc tcgagcaggt gatgtgccgc agcctggtca
     3901 gcgtggactg gctgggctat ctgtacgact gcgatttcaa ccagcagctc gggctgccga
     3961 tccgcgatgc cggcaagccg cgggtgcacc tggccgaaat cacggccagc gcgctcgaag
     4021 gccagccgat tcgcgtggcc aatcactgct tcggctgcac cgccggccaa ggctccagct
     4081 gtggcggcgc cctggaggcc gccacggcgt ccgcaccggg ctgtggcatc tgatcgggcg
     4141 tcaattgccg cggtatcgcc tcagccgccc gggagccacg gctcggtctt ccggtgcgct
     4201 tcgatgccga tcgctggcgc gccaactttc tggcgcaatg gcccctcgag gagagcgaat
     4261 cgctttgagg aagtggcggc ctgtcgtctc cttcacgatg ctgc
//
LOCUS       DASWIY010000057        16345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1150101, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000057 DASWIY010000000
VERSION     DASWIY010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 16345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16345
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150101"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1138)
                     /gene="pdxA"
                     /locus_tag="VGA59_02100"
     CDS             complement(137..1138)
                     /gene="pdxA"
                     /locus_tag="VGA59_02100"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX9718496.1"
                     /translation="MERPIVLITMGDPTGVGPEVIVKSVNQPAVQSRLRPLVIGDTRR
                     LRKAVELTGVRLAVHTIHRPEEATFEPDSIDCLDLGIVADELAFGRLSPVAGEAAYRF
                     IAKAVEVAQSGFAHAICTAPLNKEALHAAGHRYPGHTELLAHLSGTPEVSMLLVAPGL
                     RVIHVTTHIGLLDAVARIEPGLVERTIRRGNDLLVRAGIASPRIAVCGINPHAGESGL
                     FGRGEEAGKIAPAVQACVRSGLDVQGPLPADTLFYRARRGDFDLIVAMYHDQGHGPVK
                     VLGLEAGVNVTVGLPFVRTSVDHGTAFDIAGTGRADAGSMVAALELAAEFCPRGTSA"
     gene            complement(1151..2395)
                     /locus_tag="VGA59_02105"
     CDS             complement(1151..2395)
                     /locus_tag="VGA59_02105"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEX9718497.1"
                     /translation="MTIRSCCIVADDLSGAADCAASFARVTGPVPVFLGTTAEKAACL
                     AIDTDSRAMEPAAAVATTSAAFEQIGRNGAAYELVYKKIDSTLRGHVGAELVAAMGAA
                     PQFAGVVVAPSFPEQGRSLIGGKLFVQARPADNLGHSGDLMAMLAAARLRPALLGRPQ
                     QDCPQLIRGIDDALAAGARAVVVDASEQDDLARLAEALRSPRSRPLLVAGSAGLARAL
                     AAHVEPGPMSATHVRARRFLAGPVITVVGSFSKAAAAQVQQVDSSGDAQVIRLDAGQW
                     LADRHAFLRQTALGGARESLRVGSNVLFAIGGEVAQGMPRALVRAMARLTAPLLQHAA
                     ACVLTGGDTARAMFDELGIQRVVVSGEFEPGIAMARVVRDATPGFVLKAGGFGDALAL
                     QRIIRRFGHPRHRPLRRQPAPC"
     gene            complement(2536..3282)
                     /locus_tag="VGA59_02110"
     CDS             complement(2536..3282)
                     /locus_tag="VGA59_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX9718498.1"
                     /translation="MIKMALQDVDGVQSLSFDLVNRKLTAVHSAPVAQVLQHLEPLGL
                     GARLVESASTSADGEDEIDPPDNAAEARALWLLLSINAAMFVVEIVVGFLAQSTGLIA
                     DSLDMFADAAVYGLSLYAVGRAAAMKIRAAHLAGWLQLALALMALGEVVRRFIIGSEP
                     VSGLMMGMGLLALIANVTCLVLISRKRNAGAHMKASYIFSANDVIANAGVILAGFLVA
                     VTGSHYPDLVIGTVVGLIVLNGARRILRLK"
     gene            3275..3505
                     /locus_tag="VGA59_02115"
     CDS             3275..3505
                     /locus_tag="VGA59_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718499.1"
                     /translation="MIMLSSDGQSILGTAKVLSNLVAVFIGSADYSAQKTRIDNAGVR
                     GHREVFKEMGTWRRAQWRRPQPSRPPGCRAAR"
     gene            complement(3514..3777)
                     /locus_tag="VGA59_02120"
     CDS             complement(3514..3777)
                     /locus_tag="VGA59_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX9718500.1"
                     /translation="MSVYMVERSLKGISMEQLGAAQQRAIRTAQEMTAEGTPIRYIRS
                     TFVPDSGQCMCLFDGSDTEQVKTLNQKANIPFDKVVPALDLTP"
     gene            3909..4799
                     /locus_tag="VGA59_02125"
     CDS             3909..4799
                     /locus_tag="VGA59_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9718501.1"
                     /translation="MELQQIRYFLSIHSHGSFSRAASECAVSQPALTASIKKLENEIG
                     GPLFYREGKRLLMTPLGRLVRPALEQVVAGTQSARDIAQNFKLLRQAPLRLGVLGTIG
                     PTLLSRVFARFHRNYPGVELTVQEGSNPELMQQLEVGEIDLAVTSAPEPLADNFRCET
                     LFTEPYVVAFAPGNPLGRFEKVRLADVDGEPYVDRLSCELREAVMALTRQSGVNLYAT
                     FRSQREDWVESMVLAGLGFAFMPLHSVRSSQLQHRPLVEPKVERKVMAVDVRGRQRPE
                     VAELLLEEMRRFEWQAPAAA"
     gene            complement(4813..5460)
                     /locus_tag="VGA59_02130"
     CDS             complement(4813..5460)
                     /locus_tag="VGA59_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX9718502.1"
                     /translation="MCSHYQAIKERERYFRKFGVYPPPHDYVEDMWPKYAGTFIRRPP
                     EAASGDEAVAAREARLGRWGLVPWGTRDAAKQLKLSTFNARVESVAGSFTFGRAWRRA
                     QHCIVPADAIYEPDWRTGKHIPTRIARAGGEPLGIAGLWDRWESPTGPMLSFTMLTIN
                     ADEHAFMGRYHRPDDEKRMVVILREEDYDAWLTAPAQRAMDFMRQCPPADLVATG"
     gene            complement(5544..6821)
                     /locus_tag="VGA59_02135"
     CDS             complement(5544..6821)
                     /locus_tag="VGA59_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718503.1"
                     /translation="MSQSIVDVDALVDDLRMATAYASRVGLLQEAGLLDSLKAAEDTL
                     RAEGKPDMHVLTVALNNVVRVIAPLTMADLRFGRDPLSPANQKQGRLLQLCLTALALG
                     VLLLIGYFMQSLHEEQEAIQTLNQIQELRPQQKLTAVRKIAQFDQPLERPNTSYETYH
                     QKVIELMQINTKLMLSYNNAVAAAMIPLFPSPAAFARSFSSAPSMAALAPAEPPPPSD
                     GATGAAGVKPASIEAEANTANSPNGERTLDPSQLICVEETNGTIRLPLDALKYPSWMR
                     SVLSDSLSDFCFQLKVLSPTGQGTLLNESLNQLTFLPVIKEKVSLRLNWFLPFFSGVL
                     GSIILVMRNVASVRTPAVEWFPILMRLSLGGVAGVVIGWFGALAVPGLESKTALSVPF
                     ALAFLTGYSIELLFNLLDRMIRTVGDPAATRRA"
     gene            complement(7090..7251)
                     /locus_tag="VGA59_02140"
     CDS             complement(7090..7251)
                     /locus_tag="VGA59_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718504.1"
                     /translation="MTTRLKQAQDELRINLSTQQMPSLLPAQRQNLQLRADALTNEIA
                     DLRRKLRAP"
     gene            7643..8905
                     /locus_tag="VGA59_02145"
     CDS             7643..8905
                     /locus_tag="VGA59_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX9718505.1"
                     /translation="MNQALWIALSIGIVVLLGIAIWSIRRHRDPVLQVECESSIDALV
                     PSLAGLTLGTAVAGNSVEVLENGAFYDVLVERIRAAEKSVNFETFLWEEGVLGQRVAN
                     ALSDRARAGMPVRVLLDAVGSSKAGRAVTRQMRDAGCKLEFFHPRKLRNLGVINDRDH
                     RKIVIIDGREAFVGGHCIKDEWLGDAEDGEHYSDVSLRLHGPIVHSLQGAFSENWGGQ
                     TGELFVGDALFPLLEPAGDVLIHAVYAKPESSAPAVKILHHTAICLARKRIWIQNPYF
                     IPEPEAIVAFGKAVKRGVDVRVLMPSTSGSDNPMVQHAGHRNFEKLMECGVRLFEYPH
                     TLLHQKVMTVDGVWSAVGSSNFDDRSFESNDEITLAVLDTAVAQQLDAIFEKYVGRAH
                     EIELEAWRKRGVWHKLKDNAFYLLNEVL"
     gene            complement(8911..9528)
                     /locus_tag="VGA59_02150"
     CDS             complement(8911..9528)
                     /locus_tag="VGA59_02150"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX9718506.1"
                     /translation="MSIVDSLMAFLQSLQGVPAYALLFGLLLASGFGLPINEDILLLV
                     AAALTLKDVMDPLPLIALAWCGLLAADTLVFHWGRLFGVRLLRHRFMARWVPEARLAA
                     MRSTMLRYGPGYIFVVRFMPGMRTALYFAAGSLRMPYRYMLLYDGAAALVELPVLVYG
                     VRYVGGRWEEILALIQRFQGFLLSGVALVVLIVWLYSRARSKNGR"
     gene            complement(9688..10083)
                     /locus_tag="VGA59_02155"
     CDS             complement(9688..10083)
                     /locus_tag="VGA59_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX9718507.1"
                     /translation="MPRSILDEAHIHPAIRDKVANHQQWILNEVGSAVESNDVVVVGM
                     GMNPWPAKARQALDAAGQPYKYLEYGNYLNFWRERNALKMWTGWPTYPMIFVKGTLVG
                     GFTDLRALMDSGELAKMLGPKSRARKARR"
     gene            10237..11151
                     /locus_tag="VGA59_02160"
     CDS             10237..11151
                     /locus_tag="VGA59_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718508.1"
                     /translation="MAQVIEKKPPGRNSARRVLLGTALALACLAGGVYADSYTLWING
                     RNGSGAAGNYLDFSYWGPAGVAAGVNKRSVNWDGYNRISTTNAKIRDALDCYCTGNNW
                     CYIAAYSAGDLQIGYALSLYGGSKRRVKDATPNASGVCGNAGGRQTGWNIRWAAVAAG
                     AAGGTELANLGSWAVSDPLTSDLKTSTARAMYDHNQTRAKWFYMFAGAKGALYSSILP
                     GQDDEVIAYHSAGGVSGSGGGSYCNPSDWFCNDLTLGSEPNQGGQPKWAYHSVFFRDD
                     AEAFDHYTRNNWEGIVGRMRADIETYAQ"
     gene            11210..12394
                     /locus_tag="VGA59_02165"
     CDS             11210..12394
                     /locus_tag="VGA59_02165"
                     /inference="COORDINATES: protein motif:HMM:NF041940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HEX9718509.1"
                     /translation="MALAAAAVVAGLLGWQWRAADAEPKAGGNGGSGQVSVDTAALGS
                     AGLAGGTGGPLSAAGLAERRRQLALWQQRYTRAEQVYSSYRDATRYPPESRPINEHPD
                     QVRPFEPIAENMPLRDASGKPAKGLSIRTTQERVFLGGAESVKFTIEAVDGSGRTVPL
                     VIERSAAQSMPDTAALITLIRAEVPFTDNGMAPDDAAGDGRYSARLVPAAQGFAGHSG
                     TIRVLVEISADGQKGIVPFDVVYVPAVPATWVGVREALEEGSLNFYLMAQVRTPGRYV
                     ASARVYDANGAPFALLQFNDEVSAGPAEFKLTLAGVLVHDKNPAFPVRLVDVEGFLLK
                     PDTFPDREMMPRRSGEAHVSRRYDVNSFSSAEWQSEERERYLDAYGRDIEQARREIAR
                     LR"
     gene            complement(12471..12695)
                     /locus_tag="VGA59_02170"
     CDS             complement(12471..12695)
                     /locus_tag="VGA59_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718510.1"
                     /translation="MNSHWQDQLLQLVDHYGQQCAQLAAERARGTYEHPPADRAAAWA
                     AILSHLAHAPRVPSPDELAVGSREQRGQPA"
     gene            12760..13041
                     /locus_tag="VGA59_02175"
     CDS             12760..13041
                     /locus_tag="VGA59_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718511.1"
                     /translation="MNTMMYVSYCQKTASLGYRGAMDIHVEYEPGLAGEPEPAVVWFG
                     SRRLTVHAILDRWYGSGRSWWKLQTDDGSYVLRRDDDSGNWELAAVPRS"
     gene            complement(13038..14852)
                     /locus_tag="VGA59_02180"
     CDS             complement(13038..14852)
                     /locus_tag="VGA59_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9718512.1"
                     /translation="MPAESVSTGALPAPPAEKRRLVGEAATLLGRAAAPERGHLLRAV
                     AWLTLAAALEALGPAIGKLLIDSYLLPRNAQVLEMGGLLLGALVAGMAASWLRYGQLV
                     RLAGVAMRSVQRVREEVYRHVLRLPMAFFDRAITGQLVSRVTNDTEAVKMLYVQVLFV
                     LLDSLIVLAGTTVAMLWLDWRLMLVVAGLLPVVALIVALYQRLSGPAVVRNRELRSDL
                     NAQMAESIAGMQVLQASNATGRFREHFAGLNEAHYHSRWRELRANAWLLRPLLDFLNV
                     ALLALVIGVFGLRSHGGALGAVEVGILYAFMNYIARIVEPLTQITMQFSLLQQALVSA
                     GRVQALLNETGTQTAADAGRVTQGAISVERLDFAYQSGHPVLHELDLQIAAGSFHGIV
                     GHTGSGKTTLLSLLLRFYQAPAGTIRIDGTPVEAIGEDHFRASIGVVPQEPFLLAATA
                     LENIAMGRMLTRDQVEAAARAAHIHDFLAGLEQGYDTPLGEGGARLSVGQKQLLAIAR
                     ALAGEPRILFLDEATSHIDSETEQVVQRALAELRGRVTIVAIAHRLSTVRAADRIVVL
                     NHGRIAELGTHAELMAKEGGLYQRLYLLQQLEDEGEGD"
     gene            complement(14842..>16345)
                     /locus_tag="VGA59_02185"
     CDS             complement(14842..>16345)
                     /locus_tag="VGA59_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX9718513.1"
                     /translation="LYAAAYRLGVELRERLYARLTVQGAAFYQHRRTGNLMALGTNDI
                     DAVEMAAGEAMLAAFDGTLTLVLVVAAMSLGVHAGLAAAALLPFPLMALGFWWISRHV
                     HEASRRSLDRFGDLNNHVQETLAGVRTVRALGLQARSEAQFSGLATGAAQASFDAQRW
                     EAAYEPALGFTLSAATVLTLGVGGWLVWQGELTVGQLTSFSLYLAQLIWPMFAAGWVL
                     SLVQRGKAAWGRLQPVLDTPLSVDDHGSTRQVTPGPLVAQGLRFHYPGQGRPALEDVS
                     FELHPGRTLGLVGPTGAGKSTVLRLLLRHHAPEAGSLRWNGLDLQDYTLDALRAAIAW
                     VPQEAFLFSASVAENMALARPQASRDQIVQAAKLAAIHDDILRLPHGYDTPVGERGVT
                     LSGGQRQRVAIARALLTDAPLLLLDDTLSAVDTDTEARILAHLRQARAGRTTIIVSHR
                     LSAVADADRVLVLREGRVVEQGAHAALLAENGWYARQWRYQQLEATLDAG"
BASE COUNT         2842 a         5453 c         5401 g         2649 t
ORIGIN      
        1 cgtcgccggc ttcgtggcgc cctatctcgg actgcccaaa acccagtggg aagtcgtgaa
       61 cgtccctccg atgcgcgtat tcacctgggt caagccgggc gctggcccat gacgccgcgg
      121 ctgcgcgaat cgcgagctag gctgaagtgc cgcggggaca gaattccgcg gccagttcaa
      181 gtgcggcgac catgcttccg gcatcggccc gacccgtgcc cgcgatgtcg aacgcggtgc
      241 catgatcgac tgaagtgcgc acgaacggca ggccgaccgt gacgttcacg ccggcctcca
      301 gcccgagcac cttcacgggg ccatggccct ggtcgtggta catcgcgacg atcaggtcga
      361 agtcgccccg cctggcgcgg tagaacaagg tgtccgcggg cagcggcccc tgtacgtcga
      421 ggccgctgcg cacgcaggcc tgaacggccg gcgcgatctt gccggcttcc tcgccgcggc
      481 caaacaggcc gctctcgccg gcatggggat tgatcccgca caccgcgatg cgcggactgg
      541 cgatgccggc gcgcaccagc aaatcattgc cgcggcggat ggtgcgctcc accaggcccg
      601 gctcgatgcg ggcaaccgcg tccagcaggc cgatgtgcgt ggtgacgtgg atcacgcgca
      661 gcccgggggc gaccagcagc atcgacactt ccggcgtgcc gctcaggtgc gccagcagtt
      721 cggtgtggcc gggatagcgg tgacccgccg cgtgcagcgc ttccttgttc aacggcgccg
      781 tgcagatggc gtgcgcaaac ccgctctgcg ccacctccac ggctttggcg atgaagcgat
      841 aggcggcctc gccggcgaca ggcgagagcc gcccgaatgc aagctcatcc gcaacgatgc
      901 ccaggtccag gcaatcgatc gaatccggct cgaaggtcgc ctcttcgggc cggtggatcg
      961 tgtgcacggc cagccgcacg ccggtcaact cgacggcttt gcgcaggcgc cgggtgtcgc
     1021 cgatgaccag cggacgaagc cggctttgca cggccggctg gttcacgctc ttgacgatga
     1081 cttcaggtcc gacaccggtg gggtcgccca tggtgatgag gacgatgggt cgctccatgc
     1141 tcgtgctttc tcaacaaggc gccggctggc gccgaagggg tcggtggcgc gggtggccga
     1201 agcgccggat aatgcgctgc agtgccagcg cgtcgccgaa gccgcctgcc ttgaggacga
     1261 acccgggcgt ggcatcgcgc acgaccctcg ccatcgctat ccccggttcg aattctcccg
     1321 agacgaccac gcgctggatg cccagctcgt cgaacatggc gcgcgcggta tccccgccgg
     1381 tcagcacgca ggcggcggcg tgttgcagca gcggcgccgt cagtcgggcc atcgcccgca
     1441 ccagcgcgcg cggcatcccc tgcgcgactt cgccgccgat ggcgaacagc acgttgctgc
     1501 ccacgcgcag gctttcgcgg gcgccgccca gcgcggtctg ccgcaggaac gcgtgccggt
     1561 cggccagcca ctggccggca tccagacgaa tcacctgcgc gtcgcccgag gaatcgactt
     1621 gctgcacctg cgcggccgca gctttggaaa agctgcccac gacggtgatc acggggccgg
     1681 caaggaaccg ccgtgcccgc acgtgtgttg cgctcatcgg gcctggctcg acatgcgcgg
     1741 ccagcgcgcg cgccaggccg gcggaacccg cgaccagcag ggggcggctt cgaggtgaac
     1801 gcagcgcctc ggccagacgc gcgagatcgt cctgctccga cgcatccacc acgaccgcgc
     1861 gtgcgcccgc tgcgagcgca tcgtcgatcc cgcggatgag ctgcgggcaa tcctgttgcg
     1921 gccgccccag caaggccggc cgcaaccggg ccgcggccag cattgccatc aagtcgccgg
     1981 agtgccccag gttgtcggcg gggcgggctt gaacaaaaag cttgccgccg atgagcgagc
     2041 ggccttgctc tggaaatgaa ggcgccacga caacgccggc aaactgcggc gccgcgccca
     2101 tggccgcaac cagttccgcg ccaacatgcc cgcgcagggt ggagtcgatc ttcttgtaga
     2161 cgagctcata cgcggcgccg ttgcgcccga tctgctcgaa cgcggcgctc gtcgttgcca
     2221 ccgccgcagc cggctccatg gcgcggctgt cggtatcgat ggcgaggcag gcggcttttt
     2281 ccgccgttgt gcccaggaat accggcaccg gcccggtgac gcgagcgaac gacgccgcgc
     2341 agtcggcggc gccggacagg tcgtcggcaa cgatgcagca ggaacgaatg gtcatcagac
     2401 aaccgaggcg tttgcccgac tatagcgcgc gcctagtcgc gggccgagcc gctggctgac
     2461 ttcgggaacc cgctttctgt accatgtgat caagcattgc cgtgcgttgc ccctgcccga
     2521 tggcgggcaa gctcgtcatt tgagtcgcag gatgcgccgg gcaccgttca gaacgatcag
     2581 gcccactacc gtcccgatga cgaggtcagg gtagtgggat ccggtcaccg ccacaaggaa
     2641 tccggcaagg atcacgcctg cgttggcgat cacgtcattg gccgagaaga tgtagctcgc
     2701 cttcatgtgt gcgccggcgt tcctcttcct ggagatgaga accaggcagg tcacgttggc
     2761 gatcagtgcc agcaggccca tccccatcat gagaccggaa accggttcgc tgccgatgat
     2821 gaagcgacgt acgacctcgc cgagggccat aagggcaagc gccagttgca gccatcccgc
     2881 caggtgagcc gcccggattt tcatggctgc cgcgcggccg acggcgtaga gggagagacc
     2941 gtagaccgcg gcgtcggcga acatgtcgag cgagtctgcg atgaggcccg tcgattgagc
     3001 caggaagccc accacgatct cgacgacgaa catggcggcg ttgatggata gcagcaacca
     3061 cagcgccctc gcctcggccg cgttgtccgg cgggtcgatc tcgtcttcac catcggcgct
     3121 ggtacttgcg gattcgacga gcctggcgcc caggcccagc ggctccaggt gttgcagcac
     3181 ctgcgccacc ggcgcgctat gtaccgcggt gagcttgcga ttgaccaggt cgaacgacag
     3241 tgactgcacc ccatcgacat cctgcagcgc catcttgatc atgctctcct ccgatgggca
     3301 atccatcctg gggacggcga aggtgctttc gaatcttgtt gcggtgttca tcggttctgc
     3361 ggactatagc gcgcagaaaa cccgcatcga caatgccggc gtgcgcgggc atcgggaagt
     3421 cttcaaggaa atgggaactt ggcggcgggc ccaatggcgc cgaccacagc cgagccggcc
     3481 gcccggctgc cgggcggcgc ggtagcgcac ccgtcagggc gtcaggtcca gcgccggcac
     3541 caccttgtcg aaggggatgt tcgccttctg gttaagcgtt ttcacctgct cggtatcgct
     3601 gccgtcgaac aggcacatgc actggccgct gtccggtacg aacgtcgagc ggatgtaccg
     3661 gatcggtgtg ccctcggcgg tcatttcctg cgccgtgcga atggcgcgct gctgagctgc
     3721 gccgagttgc tccatcgaga tccccttgag cgagcgctca accatataga ccgacatgtg
     3781 gctctccttt gaaaatgggt ggacgggtaa gcagtctagg aacgaaattc gctctcggga
     3841 agtggttgtg gcttatcgat cgataagccg tggcgtgcta tcgtttccat ccagtggcag
     3901 gagggtccat ggagctgcag cagattcgct atttcttgtc gatccattcg cacggaagct
     3961 tctcccgcgc cgccagcgaa tgcgcggtgt cgcagcccgc gctcaccgct tcgatcaaga
     4021 agctggaaaa cgaaatcggc ggccccctgt tctatcgtga gggaaagcgc ttgctcatga
     4081 caccgctggg ccggctggtg cggcccgcgc tggagcaggt cgtcgcgggc acgcagagcg
     4141 cgcgcgacat cgcgcagaat ttcaagctgc tgcgccaggc gccattgcgc ctgggcgtcc
     4201 tgggcaccat cggccccacg ctgctgtcgc gcgtttttgc ccggttccac aggaattacc
     4261 ccggcgtgga gctcacggtg caggaaggca gcaacccgga gctgatgcaa cagctggaag
     4321 tgggagaaat cgatcttgcg gtgacgagcg cgcccgagcc gctggcggac aacttccgct
     4381 gcgaaacgct tttcacggaa ccgtacgtcg tcgcgtttgc cccgggcaac ccgctcggcc
     4441 gcttcgaaaa ggtgcgcctg gccgacgtcg acggcgagcc gtatgtggac cgcttgtcgt
     4501 gcgagctgcg tgaagcggtg atggccttga cgcggcagtc cggcgtgaat ctctacgcca
     4561 ctttccgcag ccagcgggag gactgggtcg agtccatggt gctggccggg ctggggttcg
     4621 ccttcatgcc cctgcattcg gtgcgttcca gccagttgca gcacaggccg ctcgtcgagc
     4681 ccaaggtcga gcgcaaggtc atggcggtgg acgttcgtgg caggcagcgg ccggaggtgg
     4741 cggaactgct gctcgaggag atgcgccgct tcgagtggca ggcccccgcg gcggcttagg
     4801 accgcacgcc ggtcatccgg tcgcgaccag atcagccggc gggcactgcc gcatgaaatc
     4861 catggcgcgc tgcgccggcg cggtgagcca cgcgtcgtaa tcctcctcgc gcaggatcac
     4921 gaccattcgc ttctcgtcgt ccggccggtg gtagcggccc atgaacgcgt gctcgtcggc
     4981 gttgatcgtc agcatggtga agctgagcat cgggccggtg ggcgattccc agcgatccca
     5041 caggccggca atgcccagcg gctcgccgcc ggctcgggcg atgcgcgtgg ggatgtgctt
     5101 tccggtgcgc cagtcgggct cgtagatggc gtccgcgggg acgatgcagt gctgggccct
     5161 gcgccaagcg cggccgaacg tgaacgagcc ggcgacgctc tccacccgtg cattgaaggt
     5221 ggagagcttc aactgcttcg cggcgtccct ggtgccccag ggaaccagtc cccagcggcc
     5281 caaccgggcc tcgcgcgcag ccaccgcttc gtcgcccgag gcggcttcgg gcgggcggcg
     5341 gatgaatgtg cccgcgtact tgggccacat gtcctcgacg taatcatgcg gcggcggata
     5401 aacgccgaac ttgcggaaat agcgctcgcg ttccttgatg gcctggtaat ggctgcacat
     5461 ggccctattt tgcagggccg tcccgcgcga tgggatctcc ctttgaccgc agctgaggaa
     5521 tgcccccact ggccggccgt ccttcaagcg cgcctggttg ccgccgggtc gcccaccgtg
     5581 cgaatcatcc ggtccagcag gttgaacaat aactcgatgc tgtaccccgt caggaaagcc
     5641 agagcgaacg gcaccgacag tgcggtcttg gattcgagcc caggcaccgc cagtgccccg
     5701 aaccagccga tgacaacacc cgccactccg ccgagcgaca gccgcatcag gatcggaaac
     5761 cactccacgg ccggggtgcg cacgctcgcg acattgcgca tcacgaggat gatcgagcca
     5821 agcacaccgg aaaaaaacgg caggaaccag ttcagccgca gcgatacctt ctccttgatc
     5881 accggcaaga atgtcagctg gttaagggat tcattcagca gcgtgccctg gccggttgga
     5941 gacaagacct tcaactggaa gcagaagtcg ctcagggaat cgctgagcac gctcctcatc
     6001 cacgaagggt acttgagcgc atcgagcggc aaccggatgg tgccgttggt ctcctcgaca
     6061 cagatcaact gcgatgggtc cagggttctt tcgccgttgg gcgaattggc cgtattcgct
     6121 tcagcctcga tcgacgccgg tttgacaccg gccgcaccgg ttgcgccgtc cgatgggggc
     6181 ggaggttccg caggcgccaa tgcggccatg ctcggcgcgg aggaaaacga gcgtgcgaaa
     6241 gcagcgggtg atgggaaaag cggaatcatt gccgcggcca ccgcattgtt gtacgagagc
     6301 atgagcttcg tattgatctg cataagctcg atcaccttct ggtggtacgt ctcatacgac
     6361 gtattgggcc gttccagcgg ctggtcgaac tgcgcgattt ttcgcactgc cgtcaacttc
     6421 tgctggggtc gaagctcctg gatctgattg agtgtctgga tcgcttcctg ctcttcgtgc
     6481 aaggactgca tgaaatagcc gatcagcagc aagacaccca gggccaatgc cgtcaggcaa
     6541 agctggagca accggccctg cttctggttc gccggactca gcgggtcgcg cccgaagcga
     6601 agatccgcca tcgtcaaggg agcgatcaca cgcaccacgt tgttcaacgc gacggtgagc
     6661 acgtgcatat ccggcttgcc ttcagcgcga agagtatctt ccgccgcttt caggctgtcc
     6721 agcaggccgg cctcttgcaa cagacctacg cgagacgcgt acgcggtggc cattctcagg
     6781 tcatcgacca gggcgtctac gtcaacaatc gactgggaca tggtgtacct ccggctagcc
     6841 caactggccc ctcacccacc cgtaaggcgt ggggcatgag catggggttc ccgctttgcc
     6901 gggcgggctg gcccagcacc agaactggtg ggtgaaacaa atcgaacccg gcggatgcgg
     6961 gggtacttgt gccagggcgc cgcgaacatg cagcagtgga ctggcgatca ggatcaacat
     7021 cagcacggtg cgtagcagct tcatcttgaa ctccggttgg cgcgatgccc gggccgttcg
     7081 atcgcgactt cagggcgcgc gcagttttct cctcaaatcg gcaatttcat tggtgagcgc
     7141 gtcggccctg agttgaaggt tttggcgctg cgccggcagc aggctcggca tctgctgcgt
     7201 cgaaagattg atccttaact cgtcttgcgc ctgcttcagg cgcgtcgtca tggccgcatg
     7261 ctgcgcactt gctgcgggcg tcgccacggg agccgcccca ccgcttgcat gcggcgcggg
     7321 tggacgctcc gttgccgcgg ccaatgcgag cagcgtcgtc agcgtccatt gcgtaggtgt
     7381 catatgccgt tcgcaccgga atgcgatgcg aatcaatcta gcgggttttt cgggatggcg
     7441 cctcctccag ttgaaggaga ggaccggctc gttcgcggcg ccgggctcgg ttgcccggcc
     7501 tgtcgagcag tcggccgggc aagccagacc acatccgggc gggacgcccc tgtttgtcct
     7561 gactcgcatt accatgcgct gacggtggcg attcgccagg tcccgatctc gcgacctccg
     7621 gccgcaaggg agaccacaag aaatgaatca agctctctgg attgcgctgt ccatcggcat
     7681 cgtggtgctg ctgggcatcg cgatctggtc gatccggcgc caccgtgacc cggtcctgca
     7741 ggtggaatgc gagtcgtcca tcgacgcgct ggtcccctcc cttgccggtt tgacactcgg
     7801 cacggccgtg gccgggaatt ccgtcgaggt gctggaaaac ggcgctttct acgacgtgct
     7861 ggtggaacgc atccgcgcgg ccgaaaaatc ggtgaacttc gaaacattcc tctgggagga
     7921 aggcgtgctg ggccagcgcg ttgcgaacgc cctgtcggac cgggcccgcg cgggcatgcc
     7981 cgtgcgcgtt ctgctggacg cagtcggctc gagcaaggcg ggccgggccg tcacgcgcca
     8041 gatgagggac gccggatgca agctggagtt cttccacccg agaaagctgc gcaacctggg
     8101 cgtgatcaac gaccgcgatc accgcaagat cgtgatcatc gacggccgcg aggcgttcgt
     8161 cggcggccat tgcatcaagg acgagtggct gggcgacgcg gaagacggcg agcactattc
     8221 cgacgtgagc ctgcgcctgc atggccccat cgtgcacagc ttgcagggtg ccttcagcga
     8281 aaactggggc gggcaaaccg gggagctgtt cgtcggcgat gcactgttcc ccctgctgga
     8341 gccggccggc gacgtcctga tccacgcggt gtacgccaag cccgaaagtt cggcgcctgc
     8401 cgtgaagatc ctgcaccaca cggccatctg cctggcccgc aagcgcatct ggatccagaa
     8461 cccctatttc atccccgagc ccgaggccat cgtcgctttc ggcaaagccg tcaagcgcgg
     8521 cgtggacgtg cgcgtgctga tgccctccac cagcggctcc gacaacccga tggtgcagca
     8581 tgccggccac cgcaacttcg agaaactcat ggagtgcggg gtgcggctgt tcgagtaccc
     8641 gcacacgctg ttgcaccaga aggtgatgac cgtcgacggg gtatggagcg ccgtcggctc
     8701 cagcaatttc gacgaccgtt cgttcgaatc gaacgacgag atcacgctgg ccgtcctcga
     8761 caccgcggtc gcgcagcagc tggacgcgat cttcgagaag tacgtgggca gagcgcacga
     8821 gatcgagctg gaggcctggc gcaagcgcgg cgtctggcac aagctgaagg acaacgcgtt
     8881 ttatctgctg aacgaagttc tttgacgcga tcaccttcca ttcttcgacc tcgcgcggct
     8941 gtacagccac acgatcagaa cgaccagcgc gacgcccgac agcaggaagc cctggaagcg
     9001 ctgaatcagc gccaggattt cttcccagcg cccgcccaca tagcgcacgc cgtacaccag
     9061 cacgggcagc tccaccagcg ccgccgcgcc gtcgtacagg agcatgtacc ggtaaggcat
     9121 cctgagcgaa cccgcggcaa agtacaaagc ggtgcgcatc cccggcatga agcgaacgac
     9181 gaagatgtag ccgggcccgt agcgcagcat ggtgctgcgc atggccgcca gccgtgcctc
     9241 gggcacccag cgtgccatga accggtggcg cagcaggcgc acgccgaaca ggcgccccca
     9301 atggaacacc agcgtgtcgg cggcgagcag accacaccag gcgagcgcga tcaagggcag
     9361 cgggtccatc acgtccttga gcgtgagcgc cgccgcgacc agcagcagga tgtcttcgtt
     9421 gatcggcagc ccgaacccgg aagccaacag gaggccgaac agcagcgcat aggccggaac
     9481 gccttgcagc gactgcagga atgccatcag ggaatccacg atgctcacgg ctcatttccg
     9541 gcagcgcggc cgaagagctc tgtcattgcg gccgaagtca tggatgccgg atcaagcctg
     9601 ccccgggctt gatccggggt ccggcatgac aagtcagttt ggttgtcatt gcggacccga
     9661 tccgcaatcc acgtccgcca aagaccctta ccgacgcgcc ttcctggccc ggcttttggg
     9721 gccgagcatc ttcgccaact cgccgctgtc catcagcgcc cgcagatcgg tgaatccgcc
     9781 caccagcgtg cctttgacga agatcatcgg ataggtcggc catcccgtcc acatcttcag
     9841 ggcgttgcgc tcccgccaga agttgagata gttgccgtat tcgaggtact tgtagggctg
     9901 gccggccgcg tccagcgcct gccgcgcctt cgccggccac gggttcatgc ccatccccac
     9961 cacgacaacg tcgttgcttt cgacggcgct tccgacttcg ttcaggatcc actgttgatg
    10021 gttcgcgacc ttgtcgcgaa tggccgggtg gatgtgggcc tcatcgagga ttgagcgtgg
    10081 catgcgaagc tcccaaaact ggtctcgaca gtagcacagg gtcgggaatc ctcgccactg
    10141 cgaaccagct caagctcttc acgggtttgt ccccgatcgt tccgcgaact gtgcaggaaa
    10201 gaatttgcct ccccaagctg tcttatggag gaccccatgg cgcaggttat cgaaaaaaaa
    10261 ccccccgggc gaaactccgc cagacgagtg ctcctgggga cggcgctggc gctggcctgc
    10321 ctggcgggcg gggtctatgc cgacagctac acactgtgga tcaacggccg caacggcagc
    10381 ggcgccgcgg gcaattacct ggacttcagc tactggggtc cggcaggggt cgcggcgggc
    10441 gtcaacaagc ggtccgtgaa ctgggacggc tacaaccgca tctccaccac caatgccaag
    10501 attcgcgatg cgctggattg ctactgcacc ggcaacaact ggtgctacat cgccgcgtac
    10561 agtgcgggcg acctgcagat cggctatgcg ctctcgcttt acggcggcag caaacgccgc
    10621 gtaaaagacg ccacgcccaa cgcctcgggc gtgtgcggca acgcaggcgg ccggcagacg
    10681 ggatggaaca tcaggtgggc cgccgttgcc gccggcgccg ccgggggcac ggagctcgcg
    10741 aatctcggca gctgggccgt gtcggacccg ctcacctccg atctcaagac ctccaccgct
    10801 cgcgccatgt acgaccacaa ccagacgcgc gccaagtggt tctacatgtt cgccggtgcc
    10861 aagggagccc tgtactcctc catactgccg ggccaggacg acgaggtcat cgcctatcac
    10921 tcggcgggcg gcgtctccgg cagcggcggt gggagctatt gcaacccttc cgactggttc
    10981 tgcaacgacc tcaccttggg atcggaaccg aaccagggtg ggcagcccaa atgggcttac
    11041 cactcggtct ttttccgcga cgatgcggaa gcattcgacc actacacccg aaacaactgg
    11101 gaagggatag tgggaagaat gcgcgccgac atcgaaacct acgcccagtg agcctgtagg
    11161 cgtccgacaa aggccagcca tgcgcccgcc ccgttccagg cgatgggtcg tggcccttgc
    11221 cgcggccgcc gtggtggcag gattgctggg ctggcagtgg cgggctgcgg acgcggaacc
    11281 gaaagccggc ggcaacggcg gctcgggcca ggtttcggtc gacacagccg ctttgggcag
    11341 tgcggggctg gcgggtggca ccggcggccc actcagcgcc gcgggtttgg cagagcgccg
    11401 gcggcaactg gcgctctggc agcaacgcta cacgcgcgcg gagcaggtct acagcagcta
    11461 tcgggatgcc acccgttatc cccccgaatc acgcccgatc aatgagcatc cggaccaggt
    11521 gcgcccgttc gagccgattg cggagaatat gccgctgcgc gatgcatccg gcaaacccgc
    11581 gaaagggctg agcatccgca ccacgcagga aagggtattc ctgggcggag ccgaatcggt
    11641 caagttcacc atcgaggccg tggacgggag cggccgaacc gtgccactgg tgatcgagcg
    11701 atcggcagcc caaagcatgc ccgacacggc ggcgctgatc acgctcatcc gtgccgaggt
    11761 tccattcacc gacaacggca tggcaccgga cgacgcggcg ggcgacggcc ggtacagcgc
    11821 gcggctggtt ccggcggcgc aggggttcgc cggccattcg gggacgatcc gcgtgctcgt
    11881 cgagatcagc gccgacgggc aaaagggcat cgtcccattc gacgtagtct acgtgcccgc
    11941 ggtacctgcc acctgggtgg gtgtgcgcga ggcgctggaa gaaggctcgc tgaatttcta
    12001 tctcatggcc caggtgagaa cgcccgggcg ctatgtcgcc agcgcacggg tgtacgacgc
    12061 caacggcgct ccgttcgcct tgctgcaatt caacgatgag gtatcggccg gcccggccga
    12121 attcaagtta acgctggccg gtgtgctggt tcacgacaag aatcctgctt ttcccgtgcg
    12181 gctggtcgat gtggagggtt tcctgctaaa gccggacaca ttccccgacc gcgagatgat
    12241 gccgcgccgc tccggtgaag cgcatgtcag ccggcgctac gacgtgaaca gcttttcgag
    12301 cgccgagtgg caaagcgaag agcgggagcg ctatctcgac gcgtacggcc gggatatcga
    12361 gcaggcgcgc cgggagatcg ccaggctgcg gtagcggtcg aaactgccac tcgagcagtg
    12421 gctgatgagc gcccagccgg cgcccggccc gtagcggttc cgcgatccgg tcaggcgggc
    12481 tgaccgcgct gctcccggct acccaccgcc agctcatcgg gcgatggaac ccgtggcgcg
    12541 tgggccaggt gcgacagaat ggcggcccag gcagcggcac ggtcggccgg cggatgctcg
    12601 tacgtccccc gagcgcgctc cgccgcgagc tgggcgcact gctggccata gtggtccacc
    12661 agctgcagga gttggtcttg ccaatgagaa ttcatttcag gtgccctttc cgagtctcgc
    12721 cgacgagcag cacatcgtcg actgttctcc cggatcgcga tgaacaccat gatgtacgtc
    12781 agttattgcc aaaaaaccgc ttcgctgggc tatcgtggcg ccatggacat ccatgtcgaa
    12841 tacgaaccag ggcttgcggg tgagccggag ccggcggtcg tgtggttcgg cagccggcgg
    12901 ctgacagtgc atgccatcct ggatcgctgg tatggcagcg gccgcagctg gtggaagctt
    12961 cagacggacg acggatccta cgtgctgcgc cgggacgacg acagcggcaa ctgggaactg
    13021 gcggcggtgc cccgcagtta gtccccctca ccctcgtctt ccagctgctg caacaggtag
    13081 agccgctggt agaggccgcc ttccttcgcc atcagctcgg catgcgtgcc gagttcggcg
    13141 atgcggccat ggttcagaac gacgatgcga tcggcggcgc gcaccgtcga caggcgatgc
    13201 gcgatggcga cgatggtgac gcggccgcgc agctccgcca gggcccgctg caccacttgt
    13261 tcggtttcgg agtcgatatg cgaggtggcc tcgtcgagga acaggatgcg cggctcgccc
    13321 gccagggccc gcgcgatcgc gagcagttgc ttctgcccca ccgacaggcg tgcgccgcct
    13381 tcgcccagag gcgtgtcgta gccctgctcc agcccggcaa gaaaatcgtg aatgtgcgcg
    13441 gcgcgggcgg cagcttcgac ctggtcacgg gtgagcatgc ggcccatcgc gatgttctcc
    13501 agcgccgtgg ccgccagcag gaacggctct tgcggcacca cgccgatgct cgcgcggaag
    13561 tggtcctccc cgatggcctc gaccggcgtg ccgtcgatgc ggatcgtgcc cgccggcgct
    13621 tgatagaagc gcagcagcag cgacagcagc gtcgtcttgc cgctgccggt gtgcccgacg
    13681 atgccgtgaa aacttccggc ggcgatctgc aggtccagct cgtgcaggac gggatgtccg
    13741 ctctggtagg cgaagtccag ccgctcgacg gagatggcgc cctgcgtcac gcgccccgcg
    13801 tcggccgccg tttgcgtccc ggtttcgttc agcagcgcct gcacccgccc ggccgacacc
    13861 agggcctgct gcagcagcga gaactgcatc gtgatctggg tcagcggctc cacgatccgg
    13921 gcgatgtaat tcatgaaggc atagaggatg cccacttcca cggcgcccaa cgcacccccg
    13981 tggctgcgca ggccgaacac cccgatcacc agcgcgagca acgccacgtt aaggaagtcc
    14041 agcagcggac gcaatagcca ggcattggcg cgcagctccc gccatcggga gtggtaatgg
    14101 gcctcgttga gcccggcaaa gtgctcacgg aaacggcccg tcgcgttgct ggcctgcagc
    14161 acctgcatcc cggcgatgct ctcggccatc tgcgcattga ggtcgctgcg cagctcgcgg
    14221 ttgcgcacca cggccggccc ggacagccgc tggtagagcg cgacgatcag cgccaccacc
    14281 ggcaacagcc ctgccaccac cagcatcagg cgccagtcca gccacagcat tgcgaccgtc
    14341 gtgcccgcga gcacgatcag gctgtcgagc agaacgaaca ggacctggac gtacagcatc
    14401 ttcaccgctt cggtgtcgtt ggtcacgcgg ctgaccagct ggcccgtgat cgcacggtcg
    14461 aaaaaagcca tgggcaggcg cagcacgtgc cgataaacct cctcgcggac gcgctgcacg
    14521 gagcgcatcg ccacaccggc cagccgcacc agctgcccgt agcgcaacca gctcgccgcc
    14581 atgccggcca cgagcgcgcc gagcagcagg ccgcccatct ccagaacctg cgcgttgcgc
    14641 ggcaacaggt aactgtcgat cagcagcttg ccgatcgccg gcccgagcgc ctccagtgcg
    14701 gccgccagcg tcagccacgc cacggcacgc agcaggtggc cccgctccgg cgccgcggcg
    14761 cgtcccagca gggtggccgc ttcgcccacc agcctgcgct tttcagcggg tggggctgga
    14821 agggcgccgg tactcaccga ttcagccggc atcgagggtc gcctccagtt gctggtagcg
    14881 ccactggcgc gcataccagc cgttctcggc cagcagtgcc gcatgggcgc cctgctccac
    14941 cacgcggcct tcgcgcagca cgaggaccct gtcggcgtcg gccaccgcgc tgagccggtg
    15001 actcacgatg atcgtggtgc ggcccgcgcg cgcttgccgc aggtgtgcaa ggatgcgcgc
    15061 ttcggtgtcg gtgtccaccg cggatagcgt gtcgtccagc aacagcagcg gcgcgtccgt
    15121 gagcagcgca cgcgcgatcg ccacccgctg gcgctggcct cccgacaagg tgacgccccg
    15181 ctcgcccacg ggcgtgtcgt agccgtgcgg caggcgcagg atgtcgtcgt ggatggcagc
    15241 gagcttcgcc gcctgcacga tctggtcccg cgacgcctgg ggccgcgcga gcgccatgtt
    15301 ctccgccacc gaagcggaaa acaggaaggc ctcctgcggc acccacgcga tggcggcgcg
    15361 caacgcgtcc agggtgtagt cctgcaggtc gaggccattc catcgcaggc tgccggcctc
    15421 cggcgcgtga tgacgcagca gcagccgcag cacggtggat ttgccggcgc cggtcgggcc
    15481 gaccaggccc agcgtgcgtc ccgggtgaag ctcgaaggac acgtcttcca gcgccggccg
    15541 tccctgcccc gggtaatgga agcgcaggcc ttgcgcgacc agcggccccg gcgtcacctg
    15601 ccgggtgctg ccgtgatcgt cgacgctcag gggcgtgtcc agcaccggct gcaagcggcc
    15661 ccacgcggcc ttgccccgct gcaccagcga cagcacccag ccggccgcga acatgggcca
    15721 gatcagctgg gccaggtaca ggctgaagct ggtgagctgg cccaccgtca gctccccctg
    15781 ccagaccagc cagccgccaa cccccagcgt gaggacggtt gcggcgctga gcgtgaagcc
    15841 cagcgcgggc tcgtaggcgg cttcccagcg ctgcgcatcg aagctggcct gggcggcgcc
    15901 ggtggccagc ccggagaatt gcgcttcgct gcgggcctgc aggcccaggg cgcgcaccgt
    15961 gcgcacgcct gccagcgtct cctgcacgtg attgttgagg tcgccgaagc ggtcgaggga
    16021 acgccgcgaa gcctcatgca cgtggcgcga gatccaccag aaacccaggg ccatcagcgg
    16081 aaacggcagc agggcggccg ccgcgagccc cgcgtgcacc cccagcgaca tcgcggccac
    16141 gaccagcacc agcgtcaacg tgccgtcgaa cgcggccaac atggcttcgc cggccgccat
    16201 ctccaccgcg tcgatgtcgt tggtgcccag cgccatcagg ttgccggtgc gccggtgctg
    16261 gtagaaggcg gctccttgca ccgtcagccg cgcatagagc cgctcgcgca actcgacgcc
    16321 aagccggtaa gccgccgcat aaagc
//
LOCUS       DASWIY010000058         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1891222, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000058 DASWIY010000000
VERSION     DASWIY010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891222"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VGA59_02190"
     CDS             <1..959
                     /locus_tag="VGA59_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718514.1"
                     /translation="AATTRSMERSMGRPLVRLAVATPVAFALVVPLLMLLPDLLRGPL
                     AEVSGDFGPFWLDNVREWRSAWQLVSPSFDPAAFWFVLPVVGTLVSAGRVTRHAGCRQ
                     LAWLAVAMFAALALLLSGWHVRALAFAHLFCALPIAWLLQKALTHPRGLITLRGILVY
                     LALGPLTTLFLPVASRTATAQGAESCSIRAIAGDLGELQRAASGRLLIAAPLDAGPEL
                     LYRTPHAVLGVPYHRNIAGDTALIALLSSTDDAAAKDIATKHLVDLVLVCATGPEIPA
                     YGAIARGALAVRLAGGAAPGWLMPVTLERSGNFRVYRVEESN"
     gene            1057..>2007
                     /locus_tag="VGA59_02195"
     CDS             1057..>2007
                     /locus_tag="VGA59_02195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX9718515.1"
                     /translation="MKRVAIHIATRTFPRFLLVSYLALLVGCLATADMTRVIGLDFMA
                     FWAASYLALAGEPAAAYDAARQMAVHVIAIGRSQQVAPWHYPPTYQLLILPLALLPYV
                     AAWGGWVSGTLAAYAAVIRQLAPRRPESLLLIFTFPVVLGNALLGQNGLLTSALLGAA
                     LLALPARPVLAGVLIGVLSIKPHLGLLIPIALIFGRQWRAFASAAATTLVLAACSVAV
                     LGIEPWIAFLNDIPTVRPLIDAGQVSRPNMPSVYATLISLGIAHDAAAVVHGIQAILV
                     IGAVAWTWNRAPMPLAGAVLACGAVMVPPYVFNYDLALLAI"
BASE COUNT          256 a          677 c          655 g          419 t
ORIGIN      
        1 tggcggcaac gacacgctcg atggaacgct cgatgggccg cccgctggtc cggctggccg
       61 tcgcgacacc ggtcgcgttc gcgcttgtcg tgccgctgct catgcttctg ccggacctgc
      121 tgcgcggtcc tttggcggag gtttccggcg atttcggccc gttctggctc gataacgtca
      181 gggaatggcg ttccgcctgg caactcgtct cgccgagctt cgatcccgca gccttttggt
      241 ttgtgctgcc ggtcgtcgga accctggttt cggcaggacg ggtgacgcgt cacgcgggct
      301 gtcgacagct cgcctggctg gcggtggcaa tgttcgcggc tcttgcgctg ctcctgtccg
      361 gttggcatgt ccgcgccctg gcgtttgccc atctgttctg cgccctgccc atcgcgtggc
      421 tgctgcagaa ggcgctgacg catccgcggg gcctgatcac gcttcgaggc atcctcgtct
      481 acctggcgct cggccccctg acgaccctgt tcctgcccgt cgcgtcgcgg acggcgacgg
      541 cgcagggcgc ggagtcgtgc agcattcgcg cgatcgccgg tgatcttggc gagctgcaac
      601 gtgctgcttc cggtcggctg ctgatagcgg cacccctcga tgccgggccc gagcttctct
      661 atcgcacgcc gcatgcggtt ctcggcgttc cgtatcatcg caacatcgcc ggcgacacgg
      721 ctttgatcgc attgctgtcc agtaccgacg atgcggcggc caaggacatt gcaacaaagc
      781 accttgtgga tctggtgctg gtctgcgcga ccgggcccga gatacctgcg tacggcgcca
      841 ttgcgcgcgg cgccctcgcc gtgcgcctgg caggcggggc tgcgccgggc tggctgatgc
      901 ccgtgaccct ggagcgctcc ggaaacttca gggtgtatcg cgtcgaggag agcaactgat
      961 ggtgcgacgc acgccggctc gcggtccgca cgcggcctcg cgcgggcgcc tcaccaaaga
     1021 gttgaaaagc tcccgacaat ttgtctataa tgcctcatga agcgtgtggc gattcatatt
     1081 gcgacgcgaa ccttcccgcg gttcctcctg gtcagctatc tggcccttct cgtcggctgt
     1141 ctcgccacgg cggacatgac tcgggtcatc gggctggatt tcatggcatt ttgggctgcc
     1201 tcctatctgg ccctggctgg cgagccggcg gcggcctatg acgccgcacg ccagatggcg
     1261 gtccatgtta ttgcaatcgg ccgtagccaa caggtagcgc cttggcatta cccgccgacc
     1321 taccagcttc tgatcctgcc gttggcattg ttgccttatg tcgcagcgtg ggggggctgg
     1381 gtgtcgggca cgctggcggc ctatgccgcc gttattcgac agctcgcccc acggcgtccc
     1441 gaatccctgt tgctgatttt caccttcccg gtcgtcctgg ggaacgcgct gctcggccag
     1501 aacggtctgc tgacgagcgc gctgctgggc gcggcgctgc tcgcgctgcc cgcccggccg
     1561 gtgctggccg gcgtgctcat cggcgtcctg agcatcaagc cgcatctcgg tcttctcatc
     1621 ccgatcgcgt tgatcttcgg gcggcaatgg cgggccttcg ccagcgccgc ggccaccacc
     1681 cttgtgcttg cggcatgcag cgtcgcggtg cttggcatcg agccatggat cgccttcctc
     1741 aacgacatac cgacggtacg gccactgatc gacgccggac aggtatcgcg tccgaacatg
     1801 ccgagcgtgt acgcaacgct gataagcctg ggcattgcgc atgatgctgc cgccgtcgtg
     1861 cacggcattc aggcgatcct ggtgatcggg gccgtcgctt ggacgtggaa tcgggcgccc
     1921 atgccacttg ccggcgccgt gctggcctgc ggcgcggtca tggtgccgcc ctatgtgttc
     1981 aactacgacc tcgcgctgct cgccatc
//
LOCUS       DASWIY010000059        19001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_406131, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000059 DASWIY010000000
VERSION     DASWIY010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 19001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19001
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406131"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VGA59_02200"
     CDS             complement(<1..240)
                     /locus_tag="VGA59_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718516.1"
                     /translation="MRNHQLLVSVLVALLPAAGMAAGDTVPCPDKKAGQWYRFAKTDL
                     YNKTTELLTKIDSVEGDRVIHNGGTFIADKMDNWHK"
     gene            958..3153
                     /locus_tag="VGA59_02205"
     CDS             958..3153
                     /locus_tag="VGA59_02205"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEX9718517.1"
                     /translation="MANLLKLRLGVSDDPTAFQPSLNDCLEAMLQQAELLMSDVLNGL
                     TAAAAPSGARRTPGFDDPGKQLAIQDLAANAKAVCATYRAELTRIVYEGGGKEQVHTE
                     LLRFEDLRLFEDDQLDQSIEVARALQEASLAVDDVLPALDALISTLLGWRTIQPGLNP
                     LRPDSFVRALQSCLALHVPDAAAREALIVPAAGLLGANMRKLYRELSDLLRSCGVEPA
                     VPLGGRVQRGGGASGTPVLDSVSRTLLTLDRLRKLLAGDFDAQVPKKDFLLTVPASMA
                     MLQDMKQVDELVQKLERRPKPPAPTVEPDMLAQAPVAAEAAPRLGHQLGEEVVRLMFD
                     NLALDQRLLPEFKRQLKAIEPAVLKLAQQDSRFFSDRTHPARQFLDRITQRSLAFASD
                     QEAGWRRFLATVEDAVRWLDSKVVDAETFGELMDHLQDSWVDHDQVVRQRREEAARAL
                     LHAEQRNLLAQKLAAEFGQALEGLDVADFVGDFLKGSWAQVVAEAQLSCADGSDDPYG
                     YRAMVDDMIWSVQKSTAMRGRARRLVQMIPGLLAKLREGLNRIEYPPELTARFFNNLI
                     TIHRAAVHEGRDAISKAAADAVEAQESEFSDSMQEAAALWLDSREATESGFLAEEELS
                     AERAASPAAMVEEPAGPASAGEMRTGTWVELMVMGEWVRAQLTWASPHATLFMFTSLA
                     GSAHSMSRRTLDRLRAQGKIKVVAERNVVDEALDQVAKAALQNSLERKT"
     gene            3346..5007
                     /gene="ettA"
                     /locus_tag="VGA59_02210"
     CDS             3346..5007
                     /gene="ettA"
                     /locus_tag="VGA59_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX9718518.1"
                     /translation="MAQYVFTMNRLGKIVPPKRHILKDISLSFFPGAKIGVIGLNGSG
                     KSTLLRIMAGVDKEYEGEATPMPGLSIGYLPQEPKLNPGHTVRESIEEAMGEVLAAKA
                     KLEEVYAAYADADADFDALAAEQARLEAIIATAGTDSEHQLEIAADALRLPPWEARIE
                     VLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVEWLEVYLKRFPGTVVAITHDR
                     YFLDNAAEWILELDRGVGIPWKGNYSTWLEQKEARLEAEQKSEDAHTRAMKKELEWVR
                     QNPKARQAKSKSRLARFEELSDYEYQKRNETQEIFIPVADRLGNEVIEFTGVSKSFGD
                     RVLIDNLSFKVPAGAIVGIIGPNGAGKSTLFKLVAGKEKPDSGEVKIGSTVKMAFVDQ
                     HRDELENQKTVWEDISGGLDILNVGKFQMASRAYAGRFNFNGGDQQKRVGTLSGGERG
                     RLHLAKTLIAGGNVLMLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLDRIAT
                     HILAAEGDSQWTFFDGNYQEYEADKRRRLGEEGAKPHRMRYKALK"
     gene            5230..7443
                     /locus_tag="VGA59_02215"
     CDS             5230..7443
                     /locus_tag="VGA59_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEX9718519.1"
                     /translation="MSAQSNTAYQAQYRACMKEAAAQARTLMQRLVRRAGITMPQHAA
                     MARDEQERKLLAEATRTLMKHEHALCESYPQALLAEFAEALSGGTRKSGALSFEALEL
                     MGDDQMQESVDLMRLQQMVLATVEAELADLNALVCAVQGMASVQAGRNPLRPEVYVRS
                     LHKVVMQSPVPPAVRSRWMDGLGEALGPELAGVYRELCTLLRSQGVAAAGFNVIPSPE
                     SKGASQGAGATGDAASQASTLLNVRELRRLLSGELDEPAVAAQAPSVPPPADFAVTVP
                     AAFEALRELKQVDQVMKRLQERTPAQPQPAQPRQESRGAGQALGQEVVNLMVENIAND
                     PRLLPPVQQAVRDLEPALLRLALSDPRFFNDRKHPARRLLEQMTQRSLAWQSVDAPGF
                     AGFLESLQEAVDVLLSTQVGGAEPFDFALKSLEEAWGDQQQRDRRHREQAVRALLKAE
                     QRNLLAERIARDLRARADVSAAPREINAFVLGPWSQVMAQARLSDESGAADPGAFGAV
                     VADLVWSAQPRVAGGNAAKLGKLVPELVEKLRHGLATIDYPAPATRRFLDHLAAVHRQ
                     ALKAGEQPSRPVPLSTSMSREELEAMFGDEEGGPGPWLAPTEAQHSGFMQTHQVSATK
                     PVFQPTQPGFSETQPFAAELAPTLPDADVRPGAWVEMLMDGGWSRYQVTWASPHATLF
                     MFADRNGKTHSMTRRLLDKMLRSDTLRTISGQPVVDGALDAVAQTALRNSVDIKL"
     gene            7731..8396
                     /locus_tag="VGA59_02220"
     CDS             7731..8396
                     /locus_tag="VGA59_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718520.1"
                     /translation="MNWYNLGLAGGIKNISEGAWQLAWEVSKAGTGLDHSNAIFKKAG
                     PDSRLVLYFTPSSQLLAESFGAKPCAKPSPAGLSLVAGSERAWQIHFGRVCGLRRGIE
                     AFHAFQRLLRRKRMSGRGSGDSPLNRHIRKLFAQANATPQAGTSMLLALASWAEENLS
                     EGQLHELQPGMDALRRAELEDPSSLDALLEETDQQIEQAQSLHDAAWVLLREVADWMP
                     TAR"
     gene            8446..9489
                     /locus_tag="VGA59_02225"
     CDS             8446..9489
                     /locus_tag="VGA59_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718521.1"
                     /translation="MFNPAFQIASPPLRMGLVGFADERHLCNLLGTRTRLRWVSSPAA
                     EADALWVNGEHAQPLRNHMVRVLSAKAGAPATILNLKEISRPVAFTYPLGNGYFSPPN
                     VFDPQSAESVADILRTFEAQLQRLCVELALGREIAARRYELRSPAFHLSLRGKLVGVV
                     NISGELGLSPQLTAVDMTEVRWSGRPAAACVIPPEFRLTSMAHVMWQYVGRSDENLLP
                     SRYGKGLIYYRRMPQVSARMIRDSHLVLISELSAMPQSLEQLQASTGMGQKQVSETLA
                     ALYFAGSITTDPRRAAQGKPWTRPADGPASDLNSSVPAEDPILVRPSHRRRGGGAPDT
                     VPLPALLQAQYDE"
     gene            9479..10411
                     /locus_tag="VGA59_02230"
     CDS             9479..10411
                     /locus_tag="VGA59_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718522.1"
                     /translation="MMNELCRTEAANPQASSSASAQSASMLAAWVGQLLEDVRRTHVA
                     RFQQLRPKLQSDAFAWDNALLTHSVQGWLAASRNLDFQLLRRRGFWARLTRGAEGAAR
                     EFGRTYEGVLSAAAKARQEFEMLSRDYRAHTSSERRLIVELDIESRALNREIDQGADW
                     LVELSCTIEKADELRGFSTRAMALSGELKRLRAASTLGREITLLGQNVLERRTALLEM
                     LKFDLHGFDKVWVQRLSNIRKKAVDRQVPLPALETAWEVHCELLTRLERTSAACLALQ
                     MEEQAMARRLAMLRGCLEGPATSVPAPLPSAFNF"
     gene            complement(10421..11764)
                     /locus_tag="VGA59_02235"
     CDS             complement(10421..11764)
                     /locus_tag="VGA59_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX9718523.1"
                     /translation="MPKAIRYTLLSIRELLISAGPFAVLAVGLLVLAYLWLDPAPPKR
                     VTLATGPAQSAYEEFGKRYRTALAADGIEVVLLPSQGSSDNLRLLREGKADVGFVQGG
                     SNERATTEESGLESLGSLFVEPVWLFYREASARKAGSPIAALTQLEGLRLNVGTAGSG
                     VPRLMDKLFEANKIDERRISTSELEQTPATVAFLAGELDAIVFASAPESLMVQMLLQT
                     PGVKLMDFAQSEAYSRRFPFLTPVVLPRGVVDLARDLPPADLRLVATTTALLTHEKTH
                     PALLQLFAQAARDIHGPAGWFNRAGAFPALEHSEYPVSREAERAIKGGQPFLQRYLPF
                     WLANLVERMWLALGIIIAILLPLSRIVPPLYEFRIRSRVFRWYGQLRAIEAQMQAEPG
                     NPAQLARELDALEDKVGRISVPLSYADELYALRNNINLVRRKLEENQPSARLAAS"
     gene            complement(11793..12815)
                     /locus_tag="VGA59_02240"
     CDS             complement(11793..12815)
                     /locus_tag="VGA59_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX9718524.1"
                     /translation="MARSASSPPRPSRRRAGGAITDVAGIEVGHFTDTRRPTGCTVVI
                     AREGAVAGVDVRGAAPGTRETDLLAPANLIDRVHGILLAGGAAWGLDAATGVVQWLEE
                     QGVGFPVGPARVPIVPGAVLFDLLVGDASIRPDAAAGYRACELASAKAPAEGNVGAGA
                     GAAVGKVFGMERGMKGGIGTASVTVDGITVGALVACNAMGDVIDPDTGQVIAGARTRN
                     GRSLMDTRRALLRGEPHKPLLAGTNTTIGVIATDAVITKVQACRLATVGHDGLARSIN
                     PVHTMSDGDTLFALGTGRAGRSPGMMLLGTMAAEAVALAVVRAVRAAQGLALGKLHLP
                     SAADLA"
     gene            complement(12861..13208)
                     /locus_tag="VGA59_02245"
     CDS             complement(12861..13208)
                     /locus_tag="VGA59_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEX9718525.1"
                     /translation="MSHEKYEEYQECIAACHACAVACNHCAACCLQEPDVKAMARCIG
                     LDTDCAQICHLAAAFMAGGSDHAQAACALCAEICRACGDECARHDMDHCQQCAEACRR
                     CEQECRKMAAVAA"
     gene            13552..13971
                     /locus_tag="VGA59_02250"
     CDS             13552..13971
                     /locus_tag="VGA59_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718526.1"
                     /translation="MHTKRLRRLAIHVLLAWLFVLGTGFANACVVQEQLRPSAHSAIH
                     GGAAASHTTIDCQTGHDHASHAVNPPCEQFSIERSALSQTAKQQSSSANDLWLASAPL
                     PSFVFLAMPEPPGKIRNKQERSSATIPIPIAFLRLTL"
     gene            14218..15444
                     /locus_tag="VGA59_02255"
     CDS             14218..15444
                     /locus_tag="VGA59_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9718527.1"
                     /translation="MALSAPLASAAQLGEDVQSLLEHAKARNPELALMRAEAEAAAQR
                     VQPAGALPDPVLRVELMNINNYGNDAGFNLLPSKVGETKYTLMQMLPYWGKRDLRRDV
                     AAADVQQAAARTSAAWTELAMKIKTSYARYYLAAGNERLTREILDLMARVEQIAQARY
                     AGGLVQQQDAIRAQLEQTTMRSELIMLESDKRQVRARLNALLARDAAAPMAEPRALRP
                     LPAVGALEAPALAQRARANNPQLAAEAARLRGAEKNRELTRRNRYPDFTVGLSPTQMG
                     SRITQWGVMVEVNIPLQQTTRRSQEAEAEAMVSAARSRSEALANQLVGDLGEQLAMLD
                     AARSTEALVTNQSLPQSELSLRSAMAAYENGKLDFTALLEAQRQIRKTRQDNLKAQAE
                     AQMRLAEIERIVGEDL"
     gene            15441..17060
                     /locus_tag="VGA59_02260"
     CDS             15441..17060
                     /locus_tag="VGA59_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9718528.1"
                     /translation="MKTSNLVLALLVVGGVGVGGYWLGGRTHASSHAAAGGTVLPEAL
                     SGANAAVPAKKTPKILYYRNPMGLADTSPTPKKDQMGMDYIPVYEGGDEGADQQGGAN
                     QIRISTQKIQKLGVRTEAAGLRALGKTIRAAGRVEPNERRVYSVSPKFEGYVERLHVN
                     VTGQPVAKGQPLFEVYSPELVSAQREYAIAAQGVQAMGAAGGEALAGMKQLAESSLAR
                     LRNWDLSPEQIAALVKTGEAKRTITFRSPASGIVTEKKALQGMRFMPGESLYQVTDLS
                     SVWVLADVFEQDIGLVKTGSNASVKINAYPDKVFEGKITYIYPTLKPETRTVPVRIEL
                     ANPGHLLKLAMYSQVELQVGAKGPVLTIPDSAVIDSGTRRIVLVQLQEGRFEPREVKL
                     GAHSDKYVEVKDGVKEGEQVVVAANFLIDAESNLKAAISGLGASQPEAGNAPAAAAPA
                     ARKVVGHKAQGTIDGIDLKAGTVSLNHGAIASLKWPAMTMEFKVANASLLQGLKVGAP
                     VSFEFVEHHAGDWVITSITSAAAKTAATSGR"
     gene            17075..>19001
                     /locus_tag="VGA59_02265"
     CDS             17075..>19001
                     /locus_tag="VGA59_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX9718529.1"
                     /translation="MLNKIIQWSGRNPFLVLLATLFIVIAGVVAVVKTPLDALPDLSD
                     VQVIVYTEYPGQAPQVVEDQVTYPLTSAMLAVPKSKVVRGFSFFGASFVYVIFEDGTD
                     IYWARTRVLEYLNFAAGRLPKGVTPALGPDATGVGWVYQYVLLAKNKSLAELRTIQDW
                     YVRYQLTKAPGVAEVASIGGFVQTYQVTVDPVKLRAYGIPLAKVSQVIRDSNRDVGGR
                     VVEMAETEYMVRGKGYLRGLEDIELLVVKSMGGTPVLIRDIARVELAPDERRGLTELN
                     GEGEVVSGIAMARYGQNALEVISNLKAKVEEISGGLPEGVTIQAVYDRSELIYRAINT
                     LTRTLIEESLIVAAVCIIFLLHVRSALVAILMLPVGVLIAFIAMRTLGMNSNLMSLGG
                     IAIAIGAMVDAAIVMIENAHKHIERLKPDHTVKDRADAMLNACREVGPALFFSLLIIT
                     VSFLPVFTLESQEGRLFSPLAYTKTFSMAGAALLSVTLVPVLMMMFIRGRIMPEAKNP
                     VNRFLIWIYRPIIAWVMRWKKLTILVAVIAMAVSIYPASKLGSEFMPTLNEGTLLYMP
                     ASLPGMSITKAAEVMQTQDKIIKSFPEVISVYGKAGRANTATDPAPVEMFETVINLKP
                     QEQWRPGMTTDKLIAEMD"
BASE COUNT         3387 a         6186 c         6332 g         3096 t
ORIGIN      
        1 cttgtgccag ttgtccatct tgtccgcgat gaatgttccg ccattgtgga tgacgcggtc
       61 accctccacg ctgtcgatct tcgtcagcag ctctgtcgtt ttgttgtata ggtccgtctt
      121 tgcgaagcgg taccactggc ctgccttctt gtctgggcac ggcacggtgt ctccggccgc
      181 catacctgcc gcgggcaaca gcgccaccag aacggatacg agcagttgat gatttctcat
      241 gaccggcctc cttcaatgca agcagatgat cccaaccgac aatctgtgca ccgcagtctc
      301 ctgatgccag caggacattc gccatcgacc gctgacgatg gatgcgagag tagtcctggg
      361 cgcggcgatt ctgggggcgg cgccttgact tcgatcaggg ttcggttgct tgcacgggag
      421 ggttaacccg tgagcagtga ttcaacgccg aaacgctcat caccctgccc accgccattg
      481 cactggctgc cgtgcggccg tcgcttgcac aggcacagcg cgtggctcgc atcggtaatg
      541 tgactggcgt ggtaaatctg acgggcgatc tggtcggcaa gcatatcgag ctggcgcgtc
      601 gccgggactc tcgcccacag gcggcgatgc tgccggatcg tcagcctcgg ccgtcattgg
      661 cctttttctt ggcacacgac tgtggttcgg cgcggcaccg gatggtcgcg ggacgcctgg
      721 gtgtccgccc gggtgtccgt tttgccccat ttccccagcc gatccagcgg gcgccgggat
      781 tgtgtctcaa atttgccgca tcgttacggc cgcttacacc cccctgcagg tgttgagttc
      841 gagtcgcgca aggctgggaa agccatcctt gttgctcgcc tggccgggca acgccgggaa
      901 aagactggcg ccggtcgagc tgccggacag ataatttggt ttgccttcca taaaaccatg
      961 gccaacctgc tgaagcttcg actcggcgtt tccgacgacc ccacggcgtt ccagccgtcg
     1021 ctcaatgact gcctggaggc catgctccag caggccgagt tgctgatgag cgacgtgctc
     1081 aatggcctca cggccgcggc cgcgccatcc ggcgcacgcc gcacccccgg cttcgatgac
     1141 cctggcaagc aactggcgat ccaggacctg gccgccaatg cgaaagccgt ctgcgccact
     1201 tatcgcgctg aactgacgcg catcgtctat gagggcggcg gcaaggaaca agtgcatacc
     1261 gaactgctgc gcttcgaaga cctgcgtctg ttcgaggacg atcagctcga ccagagcatc
     1321 gaagtggcac gcgccctgca ggaagcctcg ctggccgtgg atgacgtgct gccggcgctg
     1381 gacgcgctga tcagcacctt gctcggctgg cgcacgatcc agcccggcct gaaccccttg
     1441 cggcccgaca gcttcgtgcg agcgctgcag tcctgcctgg cgctgcatgt gcccgacgca
     1501 gccgcgcgcg aagcgctgat cgtgccggcc gccggcttgc tgggcgccaa catgcgcaag
     1561 ctctaccgcg agctgtcgga cctgctgcgc tcctgcggcg tggagccggc ggtacctctc
     1621 ggcggcaggg tgcaaagggg cggcggcgcc agtggcacgc ccgtgctcga ttcggtgtcc
     1681 aggaccttgc tgacgctgga ccggctgcgc aagctcctgg ccggcgactt cgatgcgcaa
     1741 gtgcccaaga aggatttcct gctcacggtg ccggcgtcca tggcgatgct gcaggacatg
     1801 aagcaggtcg acgagctggt gcagaagctg gagcggcggc ccaaaccacc ggcgcccacc
     1861 gtcgagccgg acatgctggc acaggcgccc gtggctgcgg aagccgcgcc gcgactgggc
     1921 caccagctgg gcgaggaggt ggtgcgcctg atgttcgaca acctggcgct ggaccagcgg
     1981 ttgctgcccg agttcaagcg ccagctcaag gccatcgagc cggccgtgct gaagctggcg
     2041 cagcaggact cccgtttttt cagcgaccgg acccatccgg cgcgccagtt cctcgaccgc
     2101 atcacccagc gcagcctcgc gttcgcgtcc gaccaggaag cggggtggcg ccgctttctc
     2161 gccacggtcg aggatgcggt ccgctggctc gacagcaagg tggtcgacgc agagaccttc
     2221 ggcgaattga tggaccacct gcaggacagt tgggtcgatc acgaccaggt ggtgcgccag
     2281 cgccgcgagg aagccgcgcg cgccctgttg cacgccgagc agcgcaacct gctggcgcag
     2341 aagctggccg ccgagttcgg ccaagcgctg gaagggctgg acgtcgcgga cttcgtgggc
     2401 gatttcctca agggctcctg ggcgcaggtg gtggccgagg cccagctcag ttgcgcggat
     2461 ggctcggacg atccctacgg ctatcgcgcg atggtcgacg acatgatctg gagcgtgcag
     2521 aagagcacgg cgatgcgcgg ccgcgcccgc cgcctggtgc agatgattcc gggcctgctc
     2581 gccaagctgc gcgagggcct gaatcgcatc gaatacccgc cggaactcac ggcccgtttc
     2641 ttcaacaacc tcatcaccat ccatcgcgcg gcggtgcatg aaggccgcga cgccatcagc
     2701 aaggcggcgg ccgacgccgt ggaagcccag gaatcggaat tcagcgacag catgcaggaa
     2761 gccgccgccc tgtggctgga cagccgggag gccaccgaat cgggctttct cgcggaggag
     2821 gaactctcgg ccgagcgtgc tgcaagtccg gcggcgatgg tcgaggagcc ggcgggcccc
     2881 gcttcagccg gcgaaatgcg cacaggcacc tgggtcgagc tcatggtcat gggcgagtgg
     2941 gtgcgcgcgc agctcacctg ggccagtccg cacgccaccc ttttcatgtt cacgtcgctg
     3001 gccggttcgg ctcactccat gtcccgccgc acgctggacc ggcttcgcgc ccagggcaag
     3061 atcaaggtgg tggccgagcg caacgtcgtc gacgaggccc tcgaccaggt ggccaaggcc
     3121 gcactgcaaa acagcctcga gcgcaagacc tgaggtcccg ttctctctgc ttcggggaga
     3181 gggcaggttc tgttccctct ccctctggga gagggctagg gtgagggctc atttgggacc
     3241 cggaccctca cccctgccat ctcccggagg gagaggggga aatacatcac attcccttcg
     3301 cgcgataatc gacagtttgc acagcactct cgaagcgata tcacaatggc ccagtacgtc
     3361 tttacaatga atcgcctcgg caagatcgtt ccgccgaagc gtcacatcct caaggacatc
     3421 tcgctctcat tctttccggg ggccaagatc ggcgtgatcg gcctgaacgg ctccggaaag
     3481 tcgacgctgc tgcggatcat ggccggcgtc gacaaggagt acgaaggcga ggcgacgccg
     3541 atgccgggcc tgagcatcgg ctacctgccg caggagccca agctcaaccc cgggcacacg
     3601 gtgcgcgaga gtatcgagga agccatgggc gaggtgctgg ccgccaaggc caagctcgag
     3661 gaagtctacg cggcgtacgc cgacgccgac gccgatttcg atgcgctggc cgcggagcag
     3721 gcgcgcctgg aagcgatcat cgcgacggcc ggcacggact ccgagcacca gctcgagatc
     3781 gccgcggacg cattgcgcct gccgccgtgg gaagccagga tcgaagtact ttcgggcggc
     3841 gagaagcgcc gcgtcgcgct gtgccgtctg ctgctgtcca agcccgacat gctgctgctg
     3901 gacgagccca ccaaccacct ggacgccgaa agcgtggagt ggctggaggt gtatctcaag
     3961 cgcttcccgg ggaccgtggt ggcgatcacc catgaccgct acttcctcga taacgccgcc
     4021 gagtggatcc tcgagctcga ccggggtgtc ggcattccgt ggaagggcaa ctacagcacc
     4081 tggctggagc agaaggaggc ccgcctggag gcggagcaga aatccgagga cgcccacacc
     4141 agggccatga agaaggagct ggaatgggtg cgccagaacc cgaaggcgcg ccaggccaag
     4201 agcaagtcgc gccttgcccg cttcgaggag ctgtccgact acgagtacca gaagcgcaac
     4261 gagacgcagg aaatcttcat tccggtcgcc gatcggctgg gcaacgaggt gatcgagttc
     4321 acgggcgtca gcaaatcctt cggcgaccgc gtcctgatcg ataacctgag cttcaaggtg
     4381 cccgccggcg ccatcgtcgg catcatcggc cccaacggcg cgggcaagtc gacgctgttc
     4441 aagctggtgg ccggcaagga gaaaccggac agcggggaag tcaagatcgg ctccaccgtg
     4501 aagatggcgt tcgtggacca gcatcgggac gaactggaga accagaagac cgtgtgggaa
     4561 gacatctcgg gcggcctgga catcctcaac gtcggtaagt tccagatggc cagccgtgcc
     4621 tatgcgggcc gcttcaactt caacggcggc gaccagcaaa agcgggtggg cacgctttcg
     4681 ggcggcgagc gcggccggct gcacctggcc aagaccctga tcgccggggg caacgtgttg
     4741 atgctggacg aaccctccaa cgacctcgac gtggaaacct tgcgcgcgct cgaagacgcg
     4801 ctgctggaat tcgcgggcag cgtcatggtg atcagccacg accggtggtt cctggaccgc
     4861 atcgcgaccc atatcctggc cgccgagggc gacagccagt ggaccttctt cgacggaaac
     4921 taccaggaat acgaagccga caagcgcagg cgcctgggag aagagggcgc caagccgcac
     4981 cgaatgcgct acaaggctct taaatgactc aggcttgcgc ctgagtcgtt aagtgctgtc
     5041 aatgcaacgg accgcgcgag ggcggccgct gatttccagc gggtcagcgt gccgctggcg
     5101 cggcccctga atttccggcg attagggccc gttggcccta aactgtggtt gtcacagcag
     5161 gtcgtaggct gggacctatg atggtccgga gttcccggcc gtcacgaccc atgcacaggg
     5221 gagtcatcca tgtcggccca atccaacacg gcttaccagg cccagtaccg tgcctgcatg
     5281 aaggaggcgg cggcccaggc caggacgttg atgcagcggc tcgtgcgccg cgcgggcatc
     5341 acgatgcccc agcatgccgc catggcgcgc gacgagcaag agcgcaagct cctcgccgaa
     5401 gcgacccgta ccctcatgaa acacgagcat gcgctgtgcg aatcctatcc gcaggccctg
     5461 ctggcggagt tcgcggaggc gctttcgggc ggcacccgca agtccggcgc tctgagcttc
     5521 gaggcgctgg aactgatggg cgacgatcag atgcaggaaa gcgtggacct catgcggctg
     5581 cagcagatgg tgctggcgac cgtggaagcc gagctcgccg acctgaatgc gctggtctgc
     5641 gccgtgcaag gcatggcgag cgttcaggcc ggccgcaacc cgttgcgccc ggaggtgtat
     5701 gtgcgcagcc tgcacaaggt ggtcatgcaa agccccgtgc ccccggccgt gcgctcgcgc
     5761 tggatggacg gcctgggcga ggccctgggg ccggaactgg cgggcgtgta ccgggagctg
     5821 tgcacgctgc tgcgctcgca aggcgtggcc gcagcgggct tcaacgtcat tccgtcgccc
     5881 gagagcaagg gggcgtcgca gggcgcgggc gcgacagggg acgccgcatc gcaggcgagt
     5941 accttgctca acgtccggga acttcggcgg ctgctctcgg gtgaactgga tgagccggcc
     6001 gtcgcggcgc aggctcccag cgtgccgcca cccgccgact tcgccgtcac ggtgcccgcc
     6061 gcgttcgagg cattgcgcga gttgaagcag gtcgaccagg tgatgaaacg cctgcaggag
     6121 cgcacgccgg cgcagccgca gcccgcccag ccacggcagg aatcgcgcgg cgccggccag
     6181 gcgctggggc aggaagtggt caacctgatg gtcgagaaca tcgcgaacga cccgcggctg
     6241 ttgccgccgg tgcagcaggc cgtgcgcgac ctcgagcccg ccctgctgcg cctggcgctg
     6301 agcgatccgc gcttcttcaa cgaccgcaag catccggccc gccggctgct ggagcagatg
     6361 acgcaacgca gcctggcctg gcagtcggtc gatgcgccgg gcttcgccgg attcctcgaa
     6421 tcgctgcagg aggcggtgga cgtcttgctg tccacgcagg tgggcggggc cgagccgttc
     6481 gacttcgcat tgaaatcgct ggaagaggcc tggggcgacc agcagcagcg tgaccggcgc
     6541 catcgcgagc aggcggtgcg cgcgctcctg aaggccgagc agcgcaacct gctggctgaa
     6601 aggatcgccc gcgatttgcg ggcacgggcc gacgtgtcgg cggcgccgcg cgagatcaac
     6661 gccttcgtgc tcggcccctg gtcgcaggtc atggcccagg cgcgcctgtc ggatgaaagc
     6721 ggcgcggcgg atccgggggc cttcggggcc gtggtggccg acctggtctg gagcgcccag
     6781 ccacgcgtgg ccgggggcaa tgccgccaaa ttgggaaagc tggtgccgga actggtggag
     6841 aaacttcgcc acggactggc caccatcgac tacccggcgc cggcaacccg gcgcttcctg
     6901 gatcacctcg cggcggtcca ccggcaggcg ctgaaggcgg gcgagcagcc gagccggccg
     6961 gtgcccttgt ccaccagcat gtcgcgagaa gagctggaag ccatgttcgg cgatgaagag
     7021 ggcggcccgg gcccctggct ggcccccacg gaagcccagc attccggctt catgcagacg
     7081 caccaggtca gcgccaccaa gccggtgttc cagcccacgc agccggggtt cagcgagacg
     7141 cagcccttcg ccgccgaact ggcgcccacc ctgcccgacg cggatgtgcg gccgggtgct
     7201 tgggtcgaaa tgctgatgga cggcggctgg tcgcgctacc aggtgacctg ggccagcccg
     7261 cacgccacgc tgttcatgtt cgccgaccgc aatggcaaga cgcattccat gaccaggcgc
     7321 ctgctggaca agatgctgcg cagcgatacg ctgcgcacga tttccggcca gccggtagtg
     7381 gacggcgccc tcgacgcggt ggcgcagacc gcgttgcgca acagcgtgga catcaagctc
     7441 tgactgcttt gcgcaatcga agccgaggat catcctcagt ggtgcgccgg gctgacgcga
     7501 gctgtcaccc tcggcaggca cccaggaggc agctcagcag ctggatcacc ggtccagcgt
     7561 tagcttccct gctccaatgt cctgcgaatc cgtgacaaat caggcattag tgttacagga
     7621 tgtactagga caaaaactcc cccgagatag ggagcaagag cccatgcctc agccgcagtt
     7681 gaccgcaaga ctgagggcct catcaatggc gtgagccgcg gagaggtttt atgaactggt
     7741 acaacttggg cctcgccgga ggcatcaaga acatcagcga gggcgcgtgg cagttggcct
     7801 gggaagtttc gaaagcgggg acgggccttg accacagcaa cgccatcttc aagaaagccg
     7861 gccccgacag ccgtctggtc ctttacttca ctccatcctc ccaattgctc gcggagtcat
     7921 tcggcgccaa accgtgcgcg aaaccgtcgc cggcgggact gagcctggtc gcgggttcgg
     7981 aacgcgcctg gcaaattcac ttcggccggg tgtgcggcct gcgacgcgga atagaagctt
     8041 tccacgcatt ccaacgcttg ctccggcgca agcggatgag cgggcggggc tccggcgatt
     8101 cgccgctgaa ccggcacatt cggaagttgt tcgcgcaggc caatgcgact ccccaggcgg
     8161 ggacgtcgat gctgctggcg ctggcctcat gggccgagga aaacctgtcc gaagggcaat
     8221 tgcatgagct gcagccgggt atggatgccc tgcggcgggc tgagctcgaa gacccgtcca
     8281 gcctcgatgc cctgctggag gaaacggacc agcagatcga gcaggcgcag agcctgcatg
     8341 acgcggcgtg ggtgttgctg cgcgaagtgg ccgactggat gcccacggcg cggtagcaca
     8401 tggcaagacg tgcagccgct cgaccaacct ggccatagcc gacatatgtt caatcccgcc
     8461 tttcagattg cctcaccccc tttgcgcatg ggtctggtgg gattcgcgga cgagcggcac
     8521 ctgtgcaatc tgctgggtac gcggacccgc ctgcgctggg tcagttcacc cgctgccgag
     8581 gccgacgcgc tctgggtcaa cggcgaacat gcgcagccgt tgcgcaatca catggtgcgg
     8641 gttctctccg ccaaggcggg cgcgccggcc acgattctca atttgaagga gatcagccgc
     8701 ccggtggcgt ttacgtatcc cttgggaaac ggctacttca gcccaccgaa tgtgttcgac
     8761 ccgcagtcgg cggagagcgt cgcggacatt ctgcgtacgt tcgaggcgca gctgcagcgg
     8821 ttgtgcgttg aactggctct gggccgggaa atcgcggccc ggcgttacga gctgcgctca
     8881 ccggccttcc acctgagcct gcggggcaag ctggtcggtg tcgtgaacat cagcggcgaa
     8941 ctgggtctgt cgccccaact gacggcagtc gacatgacgg aggtgcgttg gagcggccgg
     9001 cccgcggcgg catgcgtgat cccgcccgag ttcaggctga cgtccatggc gcacgtcatg
     9061 tggcaatacg tcggaagatc cgacgagaac ctgctgccgt cgcgctacgg caaaggcctt
     9121 atctactacc gccggatgcc tcaggtgtct gcccgcatga tccgggattc gcatctggtc
     9181 ctgatctcgg aattgagcgc gatgccgcag tcactcgaac agctccaggc cagcactggc
     9241 atgggacaga agcaggtttc ggaaacgctg gcggcgcttt acttcgccgg ctccatcacc
     9301 actgatccga gaagagctgc gcaaggcaag ccttggacac gtccggccga tggaccggcg
     9361 agcgacctca attcgtccgt tcccgccgag gatcccatcc tggtgcgtcc aagccaccgc
     9421 cggcggggcg gcggtgcgcc ggacacggtg ccgctgccgg cgttgctgca ggcccaatat
     9481 gatgaatgag ctttgccgca cggaggcagc caatccgcaa gcctcgagct cggcgagcgc
     9541 ccagtcggcc agcatgttgg ccgcatgggt gggtcagcta ttggaagacg ttcgacgcac
     9601 tcacgtagcg cgattccagc agttgcggcc caagctgcag tccgatgcgt ttgcatggga
     9661 taacgccttg ctgacgcatt ctgtccaagg atggttggcg gcttcgcgca acctggactt
     9721 ccagttgctg cggcgtcgcg ggttctgggc tcgcttgacg cgtggcgctg aaggagccgc
     9781 gcgggaattc ggcaggacct acgagggcgt gttgagcgca gcggcaaagg cgaggcagga
     9841 attcgaaatg ctgtcgcgtg attaccgagc tcacacgtcg agcgagcgcc ggctcatcgt
     9901 cgaactcgac atcgaatccc gggctttgaa tcgagagatc gatcagggtg ccgattggct
     9961 ggtggagctg tcttgcacga tcgagaaagc agacgagttg cgcgggtttt caacgcgcgc
    10021 catggccctt tcgggagaac tcaagcgact tcgcgcggcg agcactttgg ggagggaaat
    10081 caccttgttg ggccagaacg tgctggaacg ccgcaccgcg ttgctggaga tgttgaagtt
    10141 cgacctgcat gggttcgaca aggtatgggt tcaacggctc tccaacatcc gcaagaaagc
    10201 ggtcgatcgg caggttccgc ttccggccct tgaaactgcg tgggaagtgc attgcgagct
    10261 gttgacccgg ctcgaacgca ccagtgcggc gtgcctggcc cttcaaatgg aagaacaggc
    10321 gatggcgcgc cggctggcga tgttgcgtgg gtgtctggaa gggccggcca cttcagtgcc
    10381 tgcgcctttg ccgagcgcgt tcaacttcta aggtcgcgtc tcaggaggcc gccagtcttg
    10441 cggacggctg gttttcttcg agcttgcgcc gcaccagatt gatgttgttg cgcagcgcat
    10501 acaactcgtc ggcgtacgac agcggcacgc tgatgcggcc caccttgtcc tcgagcgcgt
    10561 ccagctcccg ggcgagctgt gccggattgc ccggctcggc ttgcatctgc gcctcgatgg
    10621 cccgcagctg gccgtaccag cggaacacgc gcgagcggat acggaactcg tacagcggcg
    10681 gcacgatgcg cgacagcggc agcaggatcg cgatgatgat gcccagcgcc agccacatgc
    10741 gctcgaccag gttggccagc cagaacggca ggtagcgctg caggaagggc tggccgccct
    10801 tgatcgcccg ctcggcttcc cgcgataccg ggtattcgct gtgctccagc gcgggaaagg
    10861 cgccggcccg gttgaaccat ccggccgggc catgaatatc gcgcgccgcc tgcgcgaaca
    10921 gctgcagcag cgccggatgc gtcttctcgt gcgtgagcag ggccgtcgtg gttgccacca
    10981 ggcgcaggtc ggccggcggc aggtcgcgcg ccagatccac cacgccgcgc ggcagtacca
    11041 ccggcgtgag gaacggaaag cgccgcgaat acgcttcgct ctgggcgaag tccatcagct
    11101 tgacgcccgg cgtctgcagc agcatctgga ccatgaggga ttcgggcgcg gaggcgaaca
    11161 cgatcgcgtc gagctcgccc gcgaggaacg cgaccgtcgc gggcgtttgt tccagttccg
    11221 aggtcgagat gcgccgctcg tcgatcttgt tcgcctcgaa cagcttgtcc atcaggcgcg
    11281 gcacgccgct gccggcggtg cccacgttca gccttaatcc ctcgagttgc gtcagcgcgg
    11341 cgatagggct gcctgccttg cgcgccgagg cttcacggta aaacagccac accggctcca
    11401 cgaacaggct gcccagcgat tcgaggccgc tttcctcggt ggtggcgcgt tcgttgctgc
    11461 cgccctgcac gaagccgacg tcggccttgc cttcgcgcag caggcgcagg ttgtccgacg
    11521 agccctggct gggcagcagc accacctcga tgccgtcggc ggcgagcgcc gtccggtagc
    11581 gcttgccgaa ttcctcgtag gcgctttgcg ccgggccggt ggccagcgtc acgcgcttgg
    11641 ggggtgcggg gtccagccac aggtaggcca ggaccagcag ccccacggcc agcacggcga
    11701 acggcccggc ggaaatcagc agctcgcgga tcgacagcag ggtgtaccgg atcgccttgg
    11761 gcatcgggag ctcgatcggg ggcttccccg cttcacgcca ggtcggcggc cgagggcaaa
    11821 tgcagtttgc ccagggccag gccctgcgcg gcgcgcaccg cccgcaccac ggccagcgca
    11881 acggcttccg cggccatggt ccccagcagc atcatgccgg gcgacctgcc cgcccgcccg
    11941 gtgcccagcg cgaacagcgt atcgccgtcg gacatggtgt ggaccggatt gatgctgcgc
    12001 gccaggccgt cgtggcccac cgtcgccagc cggcaggcct ggactttggt gatcacggca
    12061 tccgtcgcga tcacgccaat ggtggtgttg gtgccggcca gcagcggctt gtgcggctcg
    12121 ccgcgcagca aggcgcgccg cgtgtccatc aaggacctgc cattgcgcgt tcgcgcaccg
    12181 gcgatcacct gtcccgtgtc cggatcgatc acgtcgccca tggcgttgca ggcgacgagc
    12241 gcgcccacgg tgatgccatc gaccgtgacg gaagcggtgc cgatgccgcc tttcatgccg
    12301 cgctccatgc cgaagacctt gccgacggcc gcgcccgcgc ccgcgccgac attgccttcg
    12361 gcgggtgcct tggccgacgc cagctcgcag gcgcggtagc cggccgccgc gtccggccgg
    12421 atggatgcat cacccacgag caggtcgaac aggacggcgc ccggcacgat gggcacccgc
    12481 gccggtccca ccgggaagcc cacaccttgc tcttccagcc attggacgac gccagtggcg
    12541 gcatccaggc cccaggcggc gccgcccgcc agcaggatgc cgtgcacgcg atcgatcagg
    12601 ttggcgggcg cgagcaggtc ggtttcgcgc gtgcccggcg ccgcgccgcg cacgtccacg
    12661 ccggcgaccg caccctctcg cgcgatgacg acggtgcagc cggtgggccg gcgggtgtcg
    12721 gtgaagtggc cgacctcgat gcccgcgaca tcggtgatcg caccgccggc tcggcgacgc
    12781 gaagggcggg gcggggatga agcggaacgg gccatggaaa tcgattatcc atgggcccga
    12841 ccggcggacc gcggccggtc tcaggccgcc actgctgcca tcttgcggca ctcctgctcg
    12901 cagcggcggc aggcttcggc gcattgctgg caatggtcca tgtcgtggcg cgcgcattcg
    12961 tcgccgcaag ccctgcaaat ttcggcgcac aaggcacacg cggcctgggc atggtcgctg
    13021 ccgcccgcca tgaaagccgc ggccagatgg cagatctggg cgcagtccgt gtccagaccg
    13081 atgcagcgcg ccatggcttt cacgtccggc tcctgcaggc agcaggccgc gcagtggttg
    13141 caggcgaccg cgcacgcatg gcaggcggcg atgcactcct ggtattcctc gtatttttcg
    13201 tgggacatgt cgttctcctg ggttccgcgg gcggagtcgg caggctcgat cccggtcgaa
    13261 accatattac gcccatcgca agcaggactc accgccgcgt gccgcgcatg acagacttgt
    13321 aatctgaggg tcatcgtctg gctgcgtgcg atcagtacac tgccgacatg ccgcaaacct
    13381 tgcctccggc ctggtcagac ctgccgcgac ccgactctcg tggggtcggc gcaggtcctg
    13441 gccggcgggt ggccatggag ccgaggccag ttgatccaag tcaactggcg cactcagccg
    13501 aaaagtatcc tcctggcatg tctttagcgc gcgcagcttc ccgctaaccc gatgcacacc
    13561 aaacgtcttc gtcgactggc aatccacgtg ctgctggcct ggctgttcgt cctgggcacc
    13621 ggctttgcca atgcatgcgt cgtccaggag caactgcgcc catccgcaca ttccgcgatc
    13681 cacggcgggg cggcggcgag ccacacgaca atcgactgcc agaccgggca tgaccacgcg
    13741 tcccatgcag tcaatccgcc ttgcgagcag ttcagcatcg agcgctccgc cttgtcgcaa
    13801 acagccaaac agcagagcag ctcggcgaac gatctctggc tggcatccgc accgctccca
    13861 tcgtttgttt tcctggccat gcctgagccg cccggcaaga tcaggaacaa gcaggagcgc
    13921 tcttctgcga ccatcccgat ccccatcgct ttcctgcgcc tgactttgta gcttggcgca
    13981 gccagccggt ccgcgagacg cctcgcgggc ggcgagcgac tcgactggcg cggggcaacc
    14041 tccccttgcg caaaaaaagc catcgttcca acgcggctgc cgagcaggcg tggcgaaaga
    14101 ttgcccaaga aaaatccacc aggagttact ccttttctag cgatgaagcc aattcgaaca
    14161 atacctgcgc aactgcccga ctgctggaga ttgccgctca tttcgctgat cagcgttgtg
    14221 gcactgagtg ccccgctggc cagtgccgcc caactcggcg aagacgtgca aagcctgctg
    14281 gagcatgcaa aggcccggaa cccggagctg gccctgatgc gcgcggaggc cgaggcggcc
    14341 gcgcagcgcg tgcagcccgc cggcgccttg cccgatccgg tgctgcgggt ggagctgatg
    14401 aacatcaaca actacggcaa cgacgcggga ttcaacctgc ttccgagcaa agtgggcgaa
    14461 acgaagtaca cgctcatgca gatgctgccg tactggggca agcgcgacct gcgccgcgat
    14521 gtcgccgccg ccgacgtgca gcaggccgcg gcgcgcacgt ccgcggcctg gaccgaactc
    14581 gcaatgaaga tcaagacgag ctacgcacgc tattacctgg cggccggcaa cgagcggctg
    14641 acgcgcgaga tcctggacct gatggcgcgg gtggagcaaa ttgcgcaggc gcgctacgcg
    14701 ggtggactgg tgcagcagca ggatgccatc cgggcccagc tcgaacagac caccatgcgc
    14761 tcggagctga tcatgctgga gagcgacaag cggcaggtac gcgcgcgcct gaacgccctg
    14821 ctggcgcgcg acgcggccgc gccaatggcc gagccgcgag cgctgcggcc gctgccggct
    14881 gtgggcgcac tggaggcgcc ggcgcttgcg cagcgggcgc gtgccaacaa tccccagctg
    14941 gcggctgaag cggcccgcct acgcggcgcc gaaaagaacc gcgaactcac ccgccgcaat
    15001 cgctacccgg acttcaccgt cggactgtcg cccacgcaga tgggatcgcg catcacgcag
    15061 tggggcgtga tggtcgaagt gaacattccg ctgcagcaga ccacgcgccg ctcgcaggag
    15121 gcggaggcgg aggctatggt cagcgccgcc cgttcacgtt ccgaggcgct ggccaatcag
    15181 ctggtgggcg acctgggcga acagctggcc atgctcgacg ccgcgcgaag caccgaagcc
    15241 ctggtcacga accagtcgct gccgcagtcg gaactgagcc tgcgctcggc gatggccgcc
    15301 tacgagaacg ggaagctcga cttcaccgcg ctcctcgagg cgcagcggca aatacgcaag
    15361 acccgtcagg acaacctgaa ggcccaggcc gaggcgcaga tgcgcctggc cgagattgaa
    15421 agaatcgtag gagaagacct gtgaaaacat cgaacctcgt gttggccctc ctcgtcgtgg
    15481 gtggtgtggg agtgggcggc tactggctgg gcggacggac ccatgcgagc tcgcatgcgg
    15541 cagcgggcgg tacggtattg cccgaggcgc tctcgggcgc gaacgctgcc gtgcccgcca
    15601 agaagacacc caagatcctc tactatcgca atcccatggg tctggcggat acgtccccca
    15661 cgccgaaaaa agaccagatg ggcatggact acattcccgt ctatgagggc ggagacgagg
    15721 gcgccgacca gcaaggcggc gccaaccaga tccgcatcag cacacagaaa atccagaagc
    15781 tgggtgtgag gaccgaagct gccggcctgc gggcgctcgg caagaccatc cgcgctgcgg
    15841 ggcgggtcga gcccaacgag cggcgggtct attccgtttc gccgaaattc gagggttatg
    15901 tggaacgcct gcacgtcaac gtgacaggcc agccggtggc aaagggccag ccgctgttcg
    15961 aggtctacag tcccgaactc gtgtcggccc agcgcgagta cgccatcgcg gcccagggtg
    16021 tgcaggccat gggtgccgcg ggcggtgaag cgctggccgg aatgaagcag ctcgccgagt
    16081 cgagcctggc acggctgcgc aactgggacc tgtcgccgga acagatcgcg gccctggtga
    16141 agaccggtga ggcgaagcgc accatcacct tccgctcacc cgcctcgggc atcgtcaccg
    16201 agaaaaaggc gctccagggc atgcgtttca tgccgggcga atcgctgtac caggtgacgg
    16261 atctgtcgtc ggtctgggtg ctggcggacg tcttcgagca ggacatcgga ctggtcaaga
    16321 ccggcagcaa cgccagcgtg aagatcaacg cctacccgga caaggtcttc gagggcaaga
    16381 tcacctacat ctacccgacc ctgaagccgg agacgcgcac cgtgcccgtg cgcatcgagc
    16441 tggccaatcc gggccacctg ctgaagttgg cgatgtactc ccaggtcgaa ctccaggtgg
    16501 gcgccaaggg acccgtcctc actattcccg attcggccgt gatcgacagc ggcacgcgcc
    16561 ggatcgtcct ggtccagctc caggagggcc gcttcgagcc gcgggaagtc aagctcgggg
    16621 cgcatagcga caagtatgtg gaagtgaagg atggtgtgaa ggaaggcgag caggtcgtcg
    16681 tcgcggcgaa cttccttatc gacgcggaaa gcaacctgaa ggccgcgatc agcgggctcg
    16741 gcgccagcca acccgaggcg gggaacgcac cggccgccgc cgcgccggcc gcgcgcaagg
    16801 tggtcggaca caaagcgcag gggaccatcg acggaatcga cctcaaggcc gggaccgtca
    16861 gcctgaatca cggcgccatt gcgtccttga aatggccggc gatgaccatg gagttcaagg
    16921 tcgccaatgc ctcgctgctg cagggcctga aagtgggcgc gccggtttcg ttcgagttcg
    16981 tggagcacca cgcgggtgat tgggtgatta catcgatcac gtccgcggca gcgaagactg
    17041 cggcgacctc cggccgctga tcgcggataa cgccatgctg aacaaaatca tccagtggtc
    17101 gggcaggaat cccttcctgg tcctgctcgc caccctcttc atcgtgatcg ccggcgtcgt
    17161 cgccgtggtc aagacgccgc tcgatgcgct gcccgacctg tccgatgtac aagtcatcgt
    17221 ctacacggaa tatcccggcc aggccccgca ggtggtcgag gaccaggtga cttatccgct
    17281 gacctcggcg atgctcgccg tgcccaaatc caaggtggtg cggggattct cctttttcgg
    17341 cgcgtccttc gtgtacgtca ttttcgagga cggcaccgac atctactggg cgcgcacccg
    17401 cgtgctggag tacctgaact tcgccgcggg ccgcctgccc aaaggcgtga cgccggccct
    17461 cggtccggac gccacaggtg tcggctgggt ttaccagtac gtactgctcg ccaaaaacaa
    17521 gtcactggca gaactgcgca ccatccagga ctggtacgtg cgctaccagc tcaccaaggc
    17581 gcccggcgtc gccgaggtgg cgtcgatcgg tggcttcgtg cagacctatc aggtcacggt
    17641 cgatccggtc aagctgcgcg cctacggcat accgctggcc aaggtgtcgc aggtgatccg
    17701 cgactccaat cgcgacgtgg gaggccgcgt ggtggagatg gccgagaccg agtacatggt
    17761 gcgcggcaaa ggctatctgc ggggcctgga agacatcgag ctgctggtgg tcaagagcat
    17821 gggcggaacg ccggtactga tccgcgacat cgcgcgggtc gaactcgcac ccgatgagcg
    17881 ccgcggcctc acggagctga atggcgaagg cgaggtcgtc tccggcatcg cgatggcccg
    17941 ctatggccag aacgcgctgg aagtcatcag caacctgaag gccaaggtcg aggagatctc
    18001 gggcggcctg cctgaagggg tgacgatcca ggccgtctat gaccgttcgg aattgatcta
    18061 tcgcgcaatc aacactctca cgcgcaccct gatcgaggaa agcctgatcg tggcggccgt
    18121 gtgcatcatc ttcctgctgc acgtgcgttc ggcactggtg gcaatcctga tgctgcccgt
    18181 aggcgtgctg atcgccttca tcgccatgcg cacgctgggg atgaattcga acctgatgag
    18241 cttgggcggt atcgccatcg cgatcggtgc catggtcgac gcggcaatcg tgatgatcga
    18301 gaacgcccac aagcacatcg agcggctcaa acccgaccac acggtgaagg accgtgccga
    18361 cgcgatgctt aacgcctgcc gggaagtcgg tccggcactg tttttctcgc tgctcatcat
    18421 cacggtgtcg ttcctgccgg ttttcacgct cgagtcgcag gaggggcgcc ttttctcgcc
    18481 gctcgcctac accaaaacat tctcgatggc cggcgcggcg ctgctgtcgg tcaccctggt
    18541 gccggtgctc atgatgatgt tcatacgcgg ccgcatcatg ccggaggcca agaacccggt
    18601 gaaccgtttt ctcatctgga tttaccgccc catcatcgcg tgggtgatgc gctggaagaa
    18661 gctcaccatc ctcgtcgccg tcatcgccat ggccgtctcg atctacccgg cctcaaaact
    18721 gggcagcgag ttcatgccca cgctgaacga aggcacgctg ctgtacatgc cggcttcgct
    18781 gccgggtatg tcgatcacca aggcggctga agtcatgcag acgcaggaca agatcatcaa
    18841 gagctttccc gaggtgatct cggtgtacgg aaaagccggc cgcgccaata ccgccaccga
    18901 tcccgcaccg gtcgagatgt tcgaaacggt gatcaacctc aagccgcaag agcagtggcg
    18961 ccccggcatg accacggaca agctgattgc cgagatggac a
//
LOCUS       DASWIY010000060         5625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3360170, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000060 DASWIY010000000
VERSION     DASWIY010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3360170"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /gene="dtd"
                     /locus_tag="VGA59_02270"
     CDS             <1..274
                     /gene="dtd"
                     /locus_tag="VGA59_02270"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX9718530.1"
                     /translation="DEAGKMNRSVQDIGGGLLLVSQFTLAADTSGGNRPSFTNAAAPE
                     EGRRLYDYFVAQARAAHPVVQTGEFAAHMQVHLVNDGPVTIPLRVT"
     gene            complement(350..847)
                     /gene="ybeY"
                     /locus_tag="VGA59_02275"
     CDS             complement(350..847)
                     /gene="ybeY"
                     /locus_tag="VGA59_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX9718531.1"
                     /translation="MPSKPARKASGRRDSGSLPKPDLILQFGGFPGASAHRAVLKRAA
                     VERWIAHALAGPAEIAVRIVGEPEARELNLQYRRKDYATNVLTFDYARHPVVMADLVL
                     CGPVVEREAREQRKTLQAHYAHLLIHGTLHAQGYEHETGVRDALEMESLEILLLGSLG
                     FENPY"
     gene            complement(837..1781)
                     /locus_tag="VGA59_02280"
     CDS             complement(837..1781)
                     /locus_tag="VGA59_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX9718532.1"
                     /translation="MILKHTFSPPNNTRLAHMCGPTDEHLRTVEIALQVSIAHRQEQF
                     KIEGPKAKALRALEVLQALYEIAGRPISEEKVQLMVASESAMSEDEEGAVVLHTRRTD
                     LKARSPNQSVYLDNIAHHDITFGIGPAGTGKTYLAVACAVDALERSAVQRIVLTRPAV
                     EAGEKLGFLPGDLAQKVDPYLRPLYDALYDLMGFDKVAKAFERNALEVAPLAFMRGRT
                     LNNAFVILDEAQNTTPEQMKMFLTRIGFGAKAVVTGDISQIDLPKGQLSGLIEAERIL
                     KRVKGIAHTRFTSADVMRHPLVARIVDAYDNARKSDAK"
     gene            1832..2407
                     /gene="ruvA"
                     /locus_tag="VGA59_02285"
     CDS             1832..2407
                     /gene="ruvA"
                     /locus_tag="VGA59_02285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX9718533.1"
                     /translation="MIGKLTGTLGDKNPPQVLVDCNGVGYEVNVPMSTFYDLPAVGEK
                     VTLLTHFVVREDAQVLFGFGSAAEREAFRQLIKISGVGPRTALSVLSGMSVADIAQAV
                     TAQDSGRLVKVPGIGKKTAERLLLELKGKFGPDLGPGAGGASSDDQADILQALVALGY
                     SDKEAAASLKALPKDVGVSEGIKLALKSLAK"
     gene            2416..3477
                     /gene="ruvB"
                     /locus_tag="VGA59_02290"
     CDS             2416..3477
                     /gene="ruvB"
                     /locus_tag="VGA59_02290"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX9718534.1"
                     /translation="MTIQTDDFELPPPQRVISAAPASPIEEAIERALRPKLLQEYVGQ
                     AKTREQLEIFIGAARMRGEALDHVLLFGPPGLGKTTLSHIIAHELGVNLRQTSGPVLE
                     KPKDLAALLTNLEKNDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSIKL
                     DLQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYTPEELARIVKRSAGLLKAPMDERG
                     GIEIARRSRGTPRIANRLLRRVRDYADVKGTGQITQEIAHKALAMLDVDPQGFDVMDR
                     KLLECVIHRFDGGPVGLDNIAASIGEERDTIEDVIEPYLIQQGYLQRTPRGRVATLAA
                     FRHLGVAPPRSAGELFADS"
     gene            complement(3681..5450)
                     /locus_tag="VGA59_02295"
     CDS             complement(3681..5450)
                     /locus_tag="VGA59_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HEX9718535.1"
                     /translation="MSPSQVIVFATPVFLALIAIEFAWGWARKRNTYRLNDAINSISL
                     GMLSETSNVLTQLLRVGIYGAVYTTFSIVGIDAAREFWTTWYGWLLALLFYDFCYYWR
                     HRAGHECAIFWAAHVVHHQSQDYNLSTALRQSSSNFLLDWMFYLPMAVAGVPPLVFAV
                     VALVDLLYQFWVHTEHVPKLGWFDRWFCSPSNHRVHHAVNDGYLDRNYGGVLIVWDRL
                     FGSFREEDEKCVYGTRSPLNSWDPLWSNAEVYWSLAKDSWHARSWLDKLRVWFKAPGW
                     RPADVASRFPKPAFDIGKMERYHPPVSRAVAWFGAIQFVLLLNGVALFLWHADGMPLA
                     QSAVWLAALSAGLWAVGAVVQGRLTILEVLLVEAAAVATATGAQDLMQWHLVFKPLVM
                     LIAIFYVADPLHLRRTPTGFDALLMGGLAFSLVGDCFLMFPGYFIPGLVSFLVAHLFY
                     IALFKQGLPWFPNRRALLATLALGAAMYAFLYNGLNPVLKIAVAAYVVVIGLMAAQAI
                     GRATALRDRASLAVAAGAGFFMLSDSLLATHKFAFPLPMAQFWVLATYYVAQLLIACN
                     AVPVIAGSACPDAAPNRALSLRA"
     gene            complement(5483..>5625)
                     /locus_tag="VGA59_02300"
     CDS             complement(5483..>5625)
                     /locus_tag="VGA59_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX9718536.1"
                     /translation="GRMLWLVPGHCDPTVNLYDFMIGVAGGLRRGTVERIIRVDARGA
                     AT"
BASE COUNT         1021 a         1835 c         1869 g          900 t
ORIGIN      
        1 cgacgaggcc ggcaagatga atcgcagtgt gcaggacatc ggcggcgggc tgctgctggt
       61 gagccagttc acgctggcgg cggatacctc gggcgggaat cgccccagct tcaccaatgc
      121 cgcggccccc gaggaaggcc ggcgcctgta cgactacttc gttgcgcagg cccgcgcagc
      181 tcaccccgtg gtccaaacag gcgagttcgc ggcgcacatg caggtgcacc tggtgaacga
      241 tgggccggtg acgattcctc tacgtgtcac atgacttgtc attgcggact cgatccgcaa
      301 tccatggatc ccaggtcaag cctggtcaag cccgggatga caagcgcatt caatacgggt
      361 tttcgaaacc caacgaaccc agcagcagga tctccagcga ctccatctcc agcgcgtcgc
      421 gtacgccggt ttcatgctca tagccctgcg catgcagcgt gccgtgaatg agcaagtggg
      481 cgtagtgggc ttgcagcgtc ttgcgctgtt cccgcgcttc ccgttcgacc accgggccgc
      541 acaggacgag atcggccatc acgacggggt ggcgcgcgta gtcgaatgtc aggacgttgg
      601 tggcgtagtc cttgcggcgg tactgcaggt tgagttcccg cgcctcgggc tcgccgacga
      661 tgcgcaccgc gatttccgcg ggaccggcaa gcgcatgcgc tatccagcgt tccaccgcgg
      721 cgcgcttcag taccgcgcgg tgcgcgcttg caccggggaa accgccgaac tgcaagatca
      781 ggtccggctt cggcaacgac ccggaatccc gccggccgga cgctttgcgg gccggcttac
      841 ttggcatcgc tctttctggc gttgtcgtac gcatccacga tgcgcgccac caggggatgg
      901 cgcatgacgt cggcgctggt gaaccgggta tgggcgatgc ccttgacgcg cttgaggatg
      961 cgctcggcct cgatgaggcc cgacaactgg cccttgggca gatcgatctg gctgatgtcg
     1021 ccggtcacca ccgccttggc gccaaagccg atgcgggtaa ggaacatctt catctgttcg
     1081 ggcgtggtgt tctgcgcttc gtccaggatg acgaaagcat tgttcagcgt gcggccgcgc
     1141 atgaaggcca gcggcgcgac ctccagcgca ttgcgctcga aggccttcgc caccttgtcg
     1201 aaacccatca ggtcgtagag cgcgtcgtac aacggccgca ggtacggatc gaccttctgc
     1261 gccaggtcgc ccggcaggaa gcccagcttc tcgccggctt cgaccgccgg ccgggtgagc
     1321 acgatgcgct gcaccgcgct gcgctccagc gcgtcgacgg cacaagccac ggcgagatag
     1381 gtcttgccgg tgccggccgg gccgatgccg aaggtgatgt cgtggtgcgc gatgttgtcc
     1441 aggtagacgc tctggttcgg gctgcgcgcc ttcaggtcgg tgcgccgcgt atggagcacc
     1501 acggcgcctt cctcgtcttc gctcatcgcg ctctcgctgg ccaccatcag ctgcactttt
     1561 tcttccgaaa tcggccggcc cgcgatctcg tacagtgcct gcaatacctc cagcgcccgc
     1621 agtgccttgg ccttcggtcc ctcgatcttg aactgctcct gccggtgcgc gatgctcacc
     1681 tgcagggcga tctcgacggt tcgcaggtgt tcgtcggtgg gtccgcacat atgggccagc
     1741 cgcgtgttgt tcggtggcga gaaggtgtgt ttgagaatca agtcgataat tccggtgaaa
     1801 cccgaaacgg ccaccagggc ccaggacccc aatgataggc aaattgaccg gcacgctagg
     1861 cgacaagaac ccaccccagg tgctggtgga ctgcaacggc gtcggctatg aggtgaacgt
     1921 gccgatgagc accttctacg acttgcccgc ggtcggcgag aaagtgaccc tcctcacgca
     1981 cttcgtcgtg cgcgaggacg cgcaggtgct gttcggcttt ggctccgccg ccgaacgcga
     2041 ggcctttcgg caactcatca agatttccgg cgtggggccg cgcacggcct tgtcggtcct
     2101 ctcgggcatg agcgtggccg acatcgccca ggcggtgacg gcccaggatt ccggccggct
     2161 ggtcaaggta ccgggtatcg gcaagaagac ggccgagcgc ctgctgctgg agctgaaagg
     2221 caagttcggc cccgatctgg ggccgggggc cggcggcgcc tcctcggatg accaggccga
     2281 tatcctgcag gcgctggtgg ccctcggcta cagcgacaag gaggcggccg cttcgctcaa
     2341 ggcgctgccc aaggatgtgg gcgtgagcga gggcatcaaa ctggcgctga agtcgctggc
     2401 caagtaacgg gcatcatgac gatccagacc gacgacttcg aactgccgcc gccccagcgg
     2461 gtgatctccg ccgcgcctgc gtccccgatc gaggaggcga tcgaacgggc cttgcgcccc
     2521 aagctgttgc aggaatacgt gggccaggcc aagacgcgcg aacagctgga gatcttcatc
     2581 ggcgcggcgc gcatgcgggg ggaagcgttg gaccatgtgc tgctgttcgg cccgccgggg
     2641 ctgggcaaga ccacgctgtc ccacatcatc gcgcacgagc tgggcgtgaa cctgcgccag
     2701 acgtcagggc cggtgctgga aaagcccaaa gacctggcgg ccctgctgac caacctcgag
     2761 aagaacgacg tcctcttcat cgacgagatc caccggctgt cgccggtggt ggaggaaatc
     2821 ctttaccccg cgctggagga ctaccagatc gacatcatga tcggcgaggg cccggccgcc
     2881 cggtcgatca agctggacct gcagccgttc acgctggtgg gcgcgacaac tcgcgccggc
     2941 atgctgacca acccgctgcg cgaccgcttc ggcatcgtcg cgcggctgga gttctatacc
     3001 cccgaggaac tggcgcgcat cgtcaagcgc agcgccggcc tgctcaaggc gccgatggac
     3061 gagcggggcg gcatcgagat cgcgcggcgc tcgcgcggaa cgccgcggat cgcgaaccgg
     3121 ctattgcgcc gcgtgcgcga ctatgccgac gtcaagggca cgggccagat cacgcaggag
     3181 atcgcgcaca aggcactggc gatgctggac gtggacccgc aaggcttcga cgtgatggac
     3241 cggaagctcc tggagtgcgt gatccaccgg ttcgacggcg gcccggtggg actggacaac
     3301 atcgccgcca gcatcggcga ggaacgcgac acgatcgagg acgtgatcga gccctacctg
     3361 atccagcagg gctacctgca gcgcaccccg cgtgggcgcg tggcgacgct ggccgccttc
     3421 cgtcacctgg gggtggcgcc gccaagaagc gcgggcgaac tgttcgcgga ctcctgagct
     3481 tcagaaattg tcatcccggg cttgatccgg gatccagtgc ggcgcctgca aagtaccggc
     3541 gcctgcaatg aacgctgtcg ctggattgcg ggtcaggccc gcaatgacag aaattgcggg
     3601 gctcgaccga gcctgccccg gacccgatcc gggggcatcc agtacggcgc ctgcacggaa
     3661 cgcagtccct ggattgcggg tcacgcccgc aatgacaatg ctctattcgg ggccgcatcg
     3721 ggacaggcac tgcccgcaat gacaggtacc gcgttgcagg cgatcaatag ctgcgccacg
     3781 tagtaggtgg ccaacaccca gaactgcgcc atcggcagcg gaaaggcgaa cttgtgggtg
     3841 gccagcagcg aatcgctcag catgaagaag cccgcacctg ccgccaccgc caacgacgcc
     3901 ctgtcgcgca gcgccgtcgc gcgcccgatc gcctgcgcgg ccatgaggcc gatcacgacc
     3961 acgtaagccg cgacggcgat cttcagcacc gggttcagtc cgttgtagag gaaggcgtac
     4021 atcgcggcgc ccagagccag cgtcgcgagc agggcgcgcc ggttcgggaa ccagggcagg
     4081 ccctgcttga acagggcgat atagaacagg tgcgcgacca ggaaggacac caatccggga
     4141 atgaagtagc cggggaacat caggaagcag tcgcccacca gcgaaaacgc caggcctccc
     4201 atcagcagcg cgtcgaaccc ggtcggcgtg cggcgcaggt gcagcgggtc ggccacgtag
     4261 aaaatcgcga tcagcatcac cagcggcttg aagaccagat gccattgcat caggtcctgc
     4321 gcgccggtgg ccgtggcaac ggctgcggct tcgaccagca gcacttcgag gatcgtcagc
     4381 cgtccctgca ccaccgcgcc cacggcccac aggccggccg aaagcgcggc cagccagacg
     4441 gcggactggg cgagcggcat cccgtccgcg tgccacagga acagcgccac gccgttcaac
     4501 agcagcacga actggatcgc accgaaccac gccaccgccc gactcaccgg cgggtggtag
     4561 cgctccatct tgccgatgtc gaacgccggc ttgggaaagc gcgacgccac gtcggccggc
     4621 cgccagcccg gagccttgaa ccagacacgc agcttgtcga gccagctgcg tgcgtgccac
     4681 gagtctttgg ccagcgacca gtagacctcg gcgttggacc agagcggatc ccagctgttc
     4741 agggggctgc gcgtgccgta aacgcatttc tcgtcttctt ccctgaagct gccgaacagg
     4801 cggtcccaga cgatcagcac gccgccgtag ttgcggtcca ggtacccgtc gttcaccgcg
     4861 tggtgcacgc ggtggttgga gggcgagcag aaccagcggt cgaaccagcc cagcttgggc
     4921 acatgctcgg tgtgcaccca gaactggtag agcaggtcga cgagggcgac gacggcgaac
     4981 accagtggcg gcacgccggc caccgccatc ggcaggtaga acatccagtc gagcaggaag
     5041 ttcgaactgc tttgccgcag ggcggtcgac aggttgtagt cctggctctg gtggtgcacg
     5101 acgtgcgccg cccagaagat cgcgcattcg tggcccgcgc gatgacgcca gtagtagcag
     5161 aagtcgtaga acagcagtgc cagcagccag ccgtaccagg tggtccagaa ttcacgcgcc
     5221 gcgtcgatgc caacgatgga aaaagtggtg tagaccgcgc catagatgcc cacacgcagc
     5281 agctgcgtga ggacgttgct ggtttcgctg agcatgccca agctgatgct gttgattgcg
     5341 tcgttgaggc gatacgtgtt gcgcttgcgc gcccagcccc aggcgaactc gatcgcgatc
     5401 agcgccagga agaccggcgt ggcgaagacg atgacctggc ttggcgacat gacgccattc
     5461 tggctatttg cgggacagga cgctaggtcg cggcgcctct cgcgtcaacc ctgatgatgc
     5521 gctccaccgt tcccctgcgc aggccgccgg cgactccgat catgaagtcg tagaggttca
     5581 ccgtcgggtc gcagtggccg gggacgagcc acagcatccg cccga
//
LOCUS       DASWIY010000061        18427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1513914, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000061 DASWIY010000000
VERSION     DASWIY010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 18427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18427
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513914"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VGA59_02305"
     CDS             complement(<1..734)
                     /locus_tag="VGA59_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX9718537.1"
                     /translation="MYETGKASVLADDDTFSAQVSVEARAIALLKAGALQNAILTSAN
                     FSIIATDEKGIIQLFNVGAERMLGYRAAEVVNKISPSDIHDPQEVRERAEALTLELGT
                     TIAPGFEALAFKASREIEDIYELTYICKDGSRFPAIVSITALRDDYGALIGYLLIGTD
                     NSVRKRVESELNEAMAAADKANRAKSEFLSSMSHELRTPLNAILGFAQLLETGSPAPT
                     VTQKRSIEQILKAGWYLLELINEILDL"
     gene            complement(1175..1924)
                     /locus_tag="VGA59_02310"
     CDS             complement(1175..1924)
                     /locus_tag="VGA59_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9718538.1"
                     /translation="MPVPHSPNQNHLLAALPAAEFARLAPHLELVPMPLGQAIYESGG
                     RLEHVYFPTTSIVSLLYVLEDGASAEIAVVGFEGILGISIFMGGDTTPSRAVVRSAGH
                     GYRLKAELLRQEFNRAGPVLRLLLRYTQALITQMTQTAVCNRHHSVEQQLCRALLLSL
                     DRLSGSSLTMTQELIANMLGVRREGVTEAAGHLQRAGLIRYSRGHIDVLDRPGLEKAV
                     CECYAVVKVEFDRLLSDIPRGDPFQLVGRRA"
     gene            complement(1985..2719)
                     /locus_tag="VGA59_02315"
     CDS             complement(1985..2719)
                     /locus_tag="VGA59_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX9718539.1"
                     /translation="MAYQLHYWPGIQGRGEFVRLALEAAGADYVDVARGDEASGQGLP
                     AMAHYLEDESVARPPYACPFLVDGKRIIGQTAAILMYLGPRLGLVGRSEADQLWTHQI
                     QLTIADFVTEVHDMHHPIGSSLYYEDQKHEALRRAVDFRNARLPKFLAWFEAVLQRNP
                     GAAGMPHMVGGRLSYADLSLFQLVEGLRYALPATAGKALKRTPLVVQLHSGVAGHKRV
                     AAYLASERRLPFNQEGIFRHYPELDG"
     gene            complement(2777..3592)
                     /gene="xth"
                     /locus_tag="VGA59_02320"
     CDS             complement(2777..3592)
                     /gene="xth"
                     /locus_tag="VGA59_02320"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX9718540.1"
                     /translation="MKIATFNVNGIKTRLPNLLQWLEREHPDAACLQELKALDGMFPH
                     EALREAGYRSVWRGQRSWNGVAIVARGTEPQEIRRELPGDPTDDHSRYLEAAVDGVLV
                     ACLYLPNGNPQPGPKFNYKLAWFERLIEHAAGLHGCGHPVVMAGDYNVVPTDFDIYNP
                     RSWLKDALLQPESRECYQRLLKQGWIDAIRHLHPDEAVFTFWDYFRKHWDRDAGLRID
                     HLLLNATLAPCLVQAGVDRWVRGQEHASDHAPTWVELDLSKLGKKPAARRKAG"
     gene            3759..4007
                     /locus_tag="VGA59_02325"
     CDS             3759..4007
                     /locus_tag="VGA59_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718541.1"
                     /translation="MANRFTDQAANADVLKDDGQIHMPHEIGADGTPRDRSDRTPDTR
                     GGEAKADQSKAEPKEGEAKPGNDINAAGFVKDKDAAKP"
     gene            complement(4025..4249)
                     /locus_tag="VGA59_02330"
     CDS             complement(4025..4249)
                     /locus_tag="VGA59_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718542.1"
                     /translation="MTSRRDTSPTRAKRGERPTSSVPASNDIGRNSASELVESPLAPG
                     HDPLDPHDFADGGGSGGAEAFRRSRVMPDK"
     gene            4359..4841
                     /locus_tag="VGA59_02335"
     CDS             4359..4841
                     /locus_tag="VGA59_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718543.1"
                     /translation="MTRILATLLFAAAASTALSAPAPDPDPLSSSECAAAREELDQAI
                     GEPAPNRRAQPERLARARRQAALACLGPQRGSRERSGAPQPAQAVPPAVIGVRPAQPP
                     PAIAAPQPPLAIPRAAVITTCDPAGCWDSEGRRLNNMGPLLMGPRGLCTVQGGVLNCP
                     "
     gene            4973..5275
                     /locus_tag="VGA59_02340"
     CDS             4973..5275
                     /locus_tag="VGA59_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718544.1"
                     /translation="MRKLIALLAVAGLSGCVAYGVPYGSAEVYYGNSPYYSGSPYYGN
                     AAPYVYGSGGYTYTPRYRYRDRDRDRDGVPNRYDRDRDGDGVTNRRDAYPNNPRRY"
     gene            5349..5927
                     /locus_tag="VGA59_02345"
     CDS             5349..5927
                     /locus_tag="VGA59_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718545.1"
                     /translation="MLKKFLFALVVLAAAASAVQAQSSPAKKDLVAKILKVQQPGIET
                     TARGLVEQPALELMENAASALPERVAKDRQDAVAKEIQGDVRKYMDDTFPLVRDRAVK
                     LAPGTIGALLEEKFTEDELKQIVSIMESPVYNKFQQMGDAMQKVLIDKVVADTRGMVE
                     PKVRSLEQSIAKRLGVAPAPAAQRAPSKPAAK"
     gene            5964..6785
                     /locus_tag="VGA59_02350"
     CDS             5964..6785
                     /locus_tag="VGA59_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HEX9718546.1"
                     /translation="MKTGSKVVLSIAALAAAAGVFWLVRPPAPERTAAPAPAPAAPAA
                     PASAPAAPPEPAIAQPIEPPVTQAPLEPSEIGPALTELFGRKAVMSFLQIDEFPRRFV
                     ATVDNLGRSHAPPMLWPVNPTPDRFTVVQGEGAMAIADENSQRYTPLLLLAETVDVPR
                     AVDLYVRMYPLLQRTYEELGFPRRQFNDRLIAVIDQLLATPEPKRPLNVELTEVKGPI
                     PSVRPWVRYTFADPELESLSAGQKILLRVGAVNGRRLKAKLAEIREELVRRGTRR"
     gene            6794..7999
                     /locus_tag="VGA59_02355"
     CDS             6794..7999
                     /locus_tag="VGA59_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9718547.1"
                     /translation="MLELLPAATGALAWPVVLALAWVAGELGHRWLNLPRISLYGLIG
                     FIFGHAQLGLLPATSDGLGVLVANIAFGLILFEFGYRINVRWLRTNPWLGATGVLETL
                     ATFVAVYALARWWGSPNLTALLLASLSMSTSPAALMRVINEQRSSGQVTERILHLAAL
                     NCVLAVFAFKVIVGFWTFESSGNLGLAFFNSLVVLAVSGALGAMFGVGVPGLLRLTGR
                     MSQDGTVAFAIAVILLVGLTHAFKFSPLVAALTFGLVARNRRLTLTQTQRNFGALGDL
                     LAVVLFAYVATRIEWPRVATGLGLGLALVATRLATKVAAVAALARASGISSRKGGLTG
                     IALMPMSVLVVLLLEEHTRHLGIDLVDQLAPLAAATLLLELIGPVATQQALRLAGEAP
                     QAREHGTGR"
     gene            7999..9135
                     /locus_tag="VGA59_02360"
     CDS             7999..9135
                     /locus_tag="VGA59_02360"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX9718548.1"
                     /translation="MPLEHFATSESLTLGVELELQLVSLSDFDLMDASPDMLDLLARR
                     RFPGNVVPEITESMIEISTDVHREHAGLLGQLHEIRDALVAAGDTLNVGICGGGTHPF
                     QLWSERKIFSKPRFKEVSALYGYLAKQFTIFGQHVHVGCTSGDQAMYLLHALNRYVPH
                     FIALSASSPYVQRNDTLFNSARLNSVFAFPLSGRAPFTLRWSEFENDYFAQMERTGVV
                     KSMKDFYWDIRPKPEYGTIELRVCDTPLTVDRAAALACYLQALCRSLLAGSEPAPAES
                     DYLVYNYNRFQACRFGLDGTIVHPRTYEALPLREDILTTLRKIGDHAEACGATAAVDH
                     LYETIKGGSDASLLRREFEQSGSAEGMVNAAIRAFRRGGSGPAR"
     gene            complement(9168..11312)
                     /locus_tag="VGA59_02365"
     CDS             complement(9168..11312)
                     /locus_tag="VGA59_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9718549.1"
                     /translation="MSDLSPSSSPTPEAAAGGERPASNLWLTLKKGAARLQDWFVIQS
                     VFVKAMIIIVAVLIPSTALYSYNLRQKQAAVAEQLKTMTPGELPAGGVSVFNEKLPVV
                     FGTGSVLSFLAGNPIERITVIYRPLMWNVSERFILVESQGKQYAYYPSDMETKIFADK
                     AVTAGWGAKLTFVPRADLDKDSVAMLQRLDQRFEGARPQSEKDGWKAGVSGVLSAALT
                     LGLLGFLAFHLKGQFKSLKFLEPSHVHGSIDDLVGMDDIKAEVAQIKDQYQRRAEYAE
                     YGISKPFNVMFSGPAGTGKTKLASYLAKELNLPILFHSAANLETGFVAGGSNTLSRIM
                     AMAKRRKRCIVFLDEAQDLFMKRGGHRKFDDDTQNTLLSVLDGVRSKSEAEIIWIVAS
                     NFNSESMQMDEAMLRRFQMKVDFRMPNKEERLAIIGHYLAQRADKVLPDLDLRHLVEV
                     TEGRSPADLETIVNQAGIAAVQTGDMIGAETLMRAAERVMVGNVNANTTKERERDRRI
                     IAIHEWGHFLVDFELERRRAESDWDAIQADMKTIKISLKANPRNNALGFVFHKQASNL
                     LKTKGDIEHDVRVLLGGMANEELFFGEEGTTNGAHNDITRVTKLLHHAVAEMGMYRKT
                     RLNFGALSNDGHGASRNLDEETRGIMEAQSERLYGETKALLARLTPLTEHLAGKLMDK
                     GEMSLAEALFEIRSFEAACHEFSSRAGLANAA"
     gene            complement(11838..12182)
                     /locus_tag="VGA59_02370"
     CDS             complement(11838..12182)
                     /locus_tag="VGA59_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718550.1"
                     /translation="MRFDVEGRWRNGDALKLAYMVKAAVARMRQDHVLIDLRRVATPP
                     DAEGKFLICDRLRRALTHKTKVVLVATTELVDTDDVPDGLSMCPDIAMFDAESDALDW
                     LGVDEAEKIPRG"
     gene            complement(12446..13381)
                     /locus_tag="VGA59_02375"
     CDS             complement(12446..13381)
                     /locus_tag="VGA59_02375"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9718551.1"
                     /translation="MQITAQAGGVYVIAPTPFRPDGAVDLASIDRLAAFYAASGVAGT
                     TVLGQLGEAPKLEQAEALQVASRFIQRSATPTIVGVSAPGFAAMRALARDAMDAGAAG
                     VMIAPPNTLRTDDQIVGYYAQAVAAIGPDVPFVIQDYPLTFTVVMTPSVIRRIVNDNP
                     SCVMLKHEDWPGLEKISTLREWENKGEMRRISILCGNGGLFLDFEVERGADGAMTGYC
                     FPDMLVELVRLSAQGRRDQAHDLFDAHLPLLRYEQQPGAGLAVRKYVLMRRGLLASEA
                     QRKPASAMSPRAREEVEYLLARLARVDARARLPAA"
     gene            complement(13598..14191)
                     /locus_tag="VGA59_02380"
     CDS             complement(13598..14191)
                     /locus_tag="VGA59_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718552.1"
                     /translation="MEIALWIKIAYTVQLAVILPVYAVVWGWRNFLWFSDIALIATGV
                     ALWLDSALLASMMALAVLLPELLWSASYFGRLLFGLRVTDLAGYMFDARKPRFVRALS
                     LFHVVLPAVLIWMLYELGYDPRALAGQTLLAWVVLPLTYAVTTPAHENINWVRGPDRF
                     QRRVHPLVWLAFLMLAFPIGLYLPTHFALQAVFAHAG"
     gene            complement(14327..16693)
                     /locus_tag="VGA59_02385"
     CDS             complement(14327..16693)
                     /locus_tag="VGA59_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9718553.1"
                     /translation="MRRPARTLLTGPLFDRARKALPALSATEREALAAGDVWWDAALF
                     SGHPDWNELLSLPPPRLSEEERAFLDGPVQQLCALVDDWRVSEEGDLPQEAWAFLKRH
                     KFFGMIIPKRYGGLGFSAYAHSEVVKRVSTRSITAGVTVMVPNSLGPGELLLRFGTDA
                     QKEHFLPRLADGREIPCFALTSEEAGSDAAAMRDRGVVCEGVHEGRRVLGLRVTWAKR
                     YITLAPVATLLGLAFKAEDPDHLLGGEEDLGITLALIPTRTRGVQVGRRHLPAMQAFQ
                     NGPTSGTDVFVPLDWVIGGRERIGQGWPMLMSALAAGRGISLPSLSAASACLAAAATG
                     AYAFVREQFGVPIGKFEGVRECLGRIAGIAYELEAVRRFTCSALAQGSEPAIVSAIVK
                     AHATWRMRVAVNDAMDVHGGKAIMDGPLNYLAGAYRALPVAITVEGANVLTRSLIIFG
                     QGAVRCHPHLLDEMLALQEPDEHEAREHFADAAAQHLRHAGATLVRAFVRSWSAGRVG
                     PAPHAGRGAPETRYYRQLARHCATLALASEVALLTLGGALKRRESLSARLGDMLSELV
                     FASAVLKRFADDGRPGDDVVLVQWCCDSALSRIETALDGFIRNFPNRPLAALLRVVTL
                     PWGVRQRGPTDRVTHACAELLMQHNAARERITHGVFRGCRRDGIERVERAFVLAHEVA
                     PLVRKGADAGHGRDWAAAVAAGVLTAGEAAQLRAAQDAARAAIQVDDFAPDELFRAHP
                     HPSPLPQAGEGVAPPSAGEAVVAPSPPQGESEQGGRVGVRGISEEAPH"
     gene            complement(16699..16779)
                     /locus_tag="VGA59_02390"
                     /pseudo
     CDS             complement(16699..16779)
                     /locus_tag="VGA59_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor"
     gene            complement(16943..>18427)
                     /locus_tag="VGA59_02395"
     CDS             complement(16943..>18427)
                     /locus_tag="VGA59_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9718554.1"
                     /translation="PDEDQGFYIAAVILPDGASLQRTDKVVNDVLQVIKSNPNNLDAI
                     SFAGFDFLGGGFRNNAATIFVTQKHWDERKVPVQALVGELFMKTAGLKEALVLAFNPP
                     PIFGLGNAGGFEFYIQNRGEGGSAKLSEVTQKFQGAVAQSKLLAGVQTLWRASTPQLY
                     VDVDRSRAKALGVPVDSVFDTLAATLGSHYVNDFNKYGRTWQVLMSADPSYRRQPDDI
                     GRMWVRSDKGEMVPVSAIADVRFSAGPESLDRFNNLPAVKLFGQGAPGVSSGQAIAEV
                     ERIAAATLPADFSYDWGGASFQEKRSSGTSGIALALGAVMVFLILSAQYEKWSLPLSV
                     LLALPFGTFGALAAVWLRGMTNDVYFQIGLVTLLGLAAKNAILIVEYASLKASEGMSA
                     PAAALEAARLRFRPIVMTSLAFILGVLPLAVSTGAGAGARVSVGTGVMGGMLAATFLA
                     IFFVPWFFRVLVDRRLREKRSVDELRQEARAAHEQATHGQPAWR"
BASE COUNT         3055 a         6214 c         6063 g         3095 t
ORIGIN      
        1 aggtcgagaa tctcgttgat cagctccagc aggtaccagc cggccttgag aatctgctcg
       61 atgctgcgct tctgggtcac ggtcggagcc ggcgagcccg tttccagcag ctgggcgaaa
      121 cccaggatgg cgttgagcgg cgtgcgcaat tcatggctca tgctggagag aaactccgat
      181 ttcgcgcgat tggccttgtc cgccgcggcc atggcctcgt tcagctcgga ctcgacgcgc
      241 ttgcgcaccg agttgtcggt cccgatcagg aggtagccga tgagggcgcc atagtcgtcc
      301 cgcagggccg tgatcgacac gatggcggga aaccggctgc cgtccttgca gatgtaagtc
      361 agctcgtaaa tatcctcgat ctcgcgcgaa gccttgaaag ccagcgcctc gaagcccggc
      421 gcgatggtgg ttcccagctc cagggtcaag gcctcggcac gctccctgac ttcctgcggg
      481 tcgtggatgt cgctcgggct gatcttgttc acgacctcgg cggcgcggta gcccagcatg
      541 cgttcggcgc cgacgttgaa cagctggatg atgcctttct cgtcggtggc gatgatcgag
      601 aaattggcgc tggtcaggat cgcgttctgc agcgcgcccg ccttcagaag tgcaatcgcg
      661 cgcgcctcga cgctgacctg cgcgctgaac gtgtcatcgt cggcaaggac ggaagctttt
      721 cctgtttcgt acatggcttt tttccgttgt gaacaacaaa cgcaccgcgg taagaagcgg
      781 gtgtcgggcc ggccatggaa ggtaacggcc tcgaaggatg ggaaacgtgc gcgcctggga
      841 ctttggacag tagcagtgtt ttgggtccgc gtatgtgcgc caggcaactt ataagcaggc
      901 gttggcggga ttcatgccgc gagagacccg gctggagtcc gggcgccaga ggaactggag
      961 ttcaggctcc agaggaactg gagttcaggc tccagagtaa ctggagtccg ggcgccgaag
     1021 gaactgtcat gccgggcttg acccggcatc catgaatgcg ggggtcatgg attgcggctc
     1081 gaggccgcaa tgacaaaagc gctgtcatgc cgggcttgac ggagcctgcc ccgggcttga
     1141 tccggggtcc ggcatgacag ccgaatggag acggctaagc gcgtcggccc acgagctgga
     1201 acggatcgcc gcgtggaatg tccgacagca ggcggtcgaa ctccaccttc accaccgcgt
     1261 agcactcgca caccgccttt tcgaggcccg ggcggtccag cacgtcgatg tggccgcggc
     1321 tgtagcggat caagccggcg cgctgcagat ggcccgcggc ctcggtcacg ccctcgcggc
     1381 gcacaccgag catgttggcg atcaactctt gcgtcatggt caatgaacta ccggacaggc
     1441 ggtccaggct caacaacaat gcgcggcaaa gctgctgttc gaccgaatgg tgccggttgc
     1501 ataccgccgt ctgagtcatc tgggtgatga gggcctgggt ataacgcagc aacaggcgca
     1561 gcaccgggcc ggcgcggttg aattcctgcc gcagcagttc cgccttcagc cggtaaccgt
     1621 ggcccgcgct gcgcaccacg gcgcggctgg gcgtggtgtc gccgcccatg aagatcgaaa
     1681 tgcccaggat tccttcgaag ccgaccacgg cgatttccgc cgacgcgccg tcctccagca
     1741 cgtaaagcag cgacacgatg gacgtcgtgg ggaaatacac atgctccagc cggccgccgg
     1801 actcgtagat ggcttgccca agcggcatgg gaaccagttc cagatgaggc gccagccgcg
     1861 cgaattcggc agcgggcagg gctgcgagga ggtgattctg gttcggacta tggggaacag
     1921 gcatcggcaa gctctaactg gagcattgtc cctcaatacg gccatccttg cggcgacaag
     1981 gggcctaacc gtccagttcc ggatagtgcc ggaatattcc ttcctgattg aacggcaacc
     2041 gtcgctcgct ggccaggtag gcagccacgc gcttgtgccc ggcgacgccg ctgtgcaatt
     2101 gcaccaccag cggcgttctc ttcagcgctt ttccggctgt ggccggcaag gcgtagcgca
     2161 gcccttcgac cagctggaac agcgacaggt cggcataact cagccggccg ccgaccatgt
     2221 gcggcatgcc ggctgcgcct ggattgcgtt gcagcaccgc ctcgaaccag gccaggaact
     2281 tcggcagccg ggcgttgcgg aagtccaccg cgcggcgcag ggcttcgtgc ttctgatcct
     2341 cgtaatagag gctgctgccg atcgggtggt gcatatcgtg cacctcggtc acgaagtcgg
     2401 caatggtcag ctggatctgg tgcgtccaga gctggtccgc ctcgctcctg cccaccagcc
     2461 ccagccgggg ccccaggtac atcaggatgg ccgcggtctg cccgatgatg cgcttgccgt
     2521 cgaccaggaa ggggcaggcg tacggcgggc gcgccaccga ctcgtcctcg aggtaatgcg
     2581 ccatcgcagg caatccctgc ccgctcgcct cgtcgccgcg cgcgacatcc acatagtcgg
     2641 cgccggccgc ctccagcgcc agccggacaa attcaccgcg gccctggatg ccgggccagt
     2701 aatgaagttg ataggccatt cacttctcct tcacgcacgt ggcgatcata gaaccgcctc
     2761 ggcgaggcat gaggattcag ccggccttgc gcctggccgc cggtttcttg cccagcttcg
     2821 acagatccag ctcgacccac gtcggcgcat ggtcgctcgc gtgctcctgg ccgcgcaccc
     2881 agcggtccac gcccgcctgc accaggcacg gcgcgagcgt ggcattgagc agcaggtggt
     2941 cgatgcgcag gcccgcatcc cggtcccagt gcttgcggaa gtagtcccag aacgtgaaga
     3001 ctgcctcgtc gggatgcaga tggcggatgg cgtcgatcca gccctgcttg agcagccgct
     3061 ggtagcactc gcggctttcc ggctgaagca gcgcatcctt gagccaggag cgcgggttgt
     3121 agatatcgaa gtcggtcggc acgacgttgt aatcgccggc catcaccacc gggtggccgc
     3181 agccgtgcag gccggccgca tgctcgatga gccgctcgaa ccacgcgagc ttgtaattga
     3241 atttcggccc cggctgcgga ttgccgttgg gcaggtagag gcaggccacg aggaccccgt
     3301 cgaccgccgc ctccaggtag cggctatggt cgtcggtcgg gtctcccggc agttcacggc
     3361 ggatctcctg cggttcggtg ccgcgcgcca cgatcgccac gccgttccag gagcgctggc
     3421 ctctccacac cgaccgataa cccgcctcgc gcagcgcctc gtgcggaaac atcccgtcca
     3481 gggccttgag ttcctgcagg caggcggcgt cgggatgctc gcgctccagc cactgcagca
     3541 ggttgggcag ccgcgtcttg atgccgttga cgttgaaggt ggcgatcttc atgcgcgcat
     3601 catctcgcaa gccgggccga cacggcgcgc ccgcggccat gaggtgaccg ggctgcgccg
     3661 attcgcataa ggcgctgtcc tacagccgag aacgaagccg gcgcgcatag tgtccactaa
     3721 ccaccgcggt tctacagttt ctgcagggag acacatacat ggccaaccga ttcaccgacc
     3781 aagccgccaa tgccgacgtc ctgaaggacg acggccagat ccacatgccg cacgagatcg
     3841 gcgccgatgg cacgccgcgc gaccgttccg atcgcacgcc ggacacccgg ggcggtgaag
     3901 cgaaagcgga ccagtccaag gccgaaccca aagagggtga agccaagccc ggcaacgaca
     3961 tcaacgctgc cggcttcgtc aaggacaagg acgctgccaa gccctgaggc agggccggcc
     4021 cgggtcactt gtcgggcatg actctggagc gccggaacgc ctcggcgccc ccgctgcccc
     4081 caccatccgc gaagtcgtgc ggatccaggg gatcgtggcc gggcgccagc ggcgactcga
     4141 ccagttcgct ggcactgttt cgcccgatgt cattgctcgc cggcaccgac gaggtcggcc
     4201 gttcgccgcg cttggcgcgg gtcgggctgg tatcgcgacg ggaagtcatg gcgttctcct
     4261 cgcatgggaa tccccaggtt aggcaaggca tcgcgggcac cctgtcggac aaagccgtga
     4321 gatagaaccg aattcgcgcg gacccgtcca accttgcaat gacccgaatc ctcgctaccc
     4381 tgctgttcgc ggcggcagcc tcgaccgcgc tgtccgcgcc agccccggac ccggatcctc
     4441 tgagctccag cgagtgcgcg gcggcgcgcg aggagctgga tcaagcgatc ggcgagccgg
     4501 ccccgaatcg gcgggcgcag ccggagcggc tggcgcgcgc ccgccggcag gccgccctcg
     4561 cctgcctggg cccgcagcgc ggcagccgcg aacgctcggg cgcgccccaa ccggcgcagg
     4621 ccgtgccgcc tgccgtcatc ggggtgcggc ccgcgcagcc accaccggcg atcgcggcgc
     4681 cgcagccgcc cctggcgatc ccgcgcgccg ccgtcatcac cacctgcgat ccggccggat
     4741 gctgggacag cgaaggccgg cggctgaaca atatgggccc cctcctgatg gggccacgcg
     4801 ggctttgcac tgtccagggc ggggtgctca attgcccgtg aaccgtcgcc ggctcgtgcg
     4861 gggcgttaac gcgcggacac agattcgcac acggcggaca caggccggac acccgatgcg
     4921 cgcccaatga agctgtcggg cttcgcagtt tgcccgtgac aaggaggttc ccgtgagaaa
     4981 gctcatcgca cttctggccg tggcgggtct gtctggttgc gtggcctatg gcgtgcctta
     5041 cggctcggcc gaggtgtact acggcaattc gccctattac agcggcagcc cctactacgg
     5101 caacgccgcc ccgtatgtct acggcagcgg aggctatacg tatacgcccc ggtaccggta
     5161 ccgcgaccgc gaccgcgacc gcgacggcgt ccccaaccgg tatgaccggg accgcgacgg
     5221 cgacggtgtt acgaatcgcc gggatgcgta tccgaacaac cccaggcggt actgagtaac
     5281 gtaacagcag ggcgatcgac cgcaagtccc ggccctgtcc ggctggccgc gggcgtttaa
     5341 gatcgcccat gctcaagaaa ttcctctttg cccttgtcgt cctggccgcg gccgcctcag
     5401 cggtgcaggc ccagtcctcg cccgccaaga aagatctggt cgccaagatc cttaaagtac
     5461 agcagccggg catcgaaacc acggcgcgcg gcctggtcga acagccggct ctcgaattga
     5521 tggaaaacgc ggccagcgcc ctgcccgaac gtgttgccaa ggaccggcag gatgcggtgg
     5581 ccaaggaaat ccagggcgat gtccgcaaat acatggacga cacgtttccg ctcgttcggg
     5641 accgcgccgt gaagctcgca cccggcacca tcggcgccct gctggaggag aagttcacgg
     5701 aagacgaact caagcagatc gtgtccatca tggaatcgcc cgtttacaat aaattccagc
     5761 agatgggcga cgccatgcaa aaagtgctga tcgacaaggt cgtggccgac acgcgcggca
     5821 tggtcgagcc gaaagtccgg tcccttgagc agtccatcgc aaaacggctc ggcgtcgcgc
     5881 cggcgccggc ggcgcagcgg gccccctcca aaccggctgc caagtagagc aagagcaggc
     5941 gtcccggagc gggtgatctc aggatgaaaa caggttcgaa ggtcgtgttg tccatagcgg
     6001 cgctggcagc cgcggcaggc gtgttctggc tggtccggcc gcctgctccc gagcgcaccg
     6061 cggcgccggc accggcgccg gccgcgcccg cagccccggc gagcgccccg gcagccccgc
     6121 cggaacccgc catcgcgcaa ccgatcgagc cgccggtcac acaggcgccc ctggagccgt
     6181 cggagatcgg cccggccctg accgagctgt tcgggcgcaa ggccgtgatg tccttcctgc
     6241 agatcgacga gtttccgcgc cgcttcgtcg cgaccgtcga caacctcggg cggtctcatg
     6301 cgccgccgat gctgtggccg gtcaacccca cgcccgaccg cttcaccgtc gtgcaagggg
     6361 agggcgccat ggcgatcgcc gatgaaaaca gccagcgcta cacaccgctg ctgctgctgg
     6421 cggaaaccgt ggacgtgccc cgggccgtgg acctgtacgt tcgtatgtac cccctgctgc
     6481 aacgcacgta tgaggagctt gggtttccac gccgccaatt caatgaccgc ctgatcgccg
     6541 ttatcgacca attgctggcg acgcccgagc cgaagcggcc cctgaacgtc gaactcaccg
     6601 aggtcaaggg tccgattccg tcggtacggc cctgggtgcg ctacacgttc gccgacccgg
     6661 aactcgagtc gctgtcggcc gggcagaaga tcctgctgcg ggtgggcgcg gtgaacgggc
     6721 gccgcctcaa ggccaagctg gccgagatcc gggaagagct cgtgcggcgt ggcaccaggc
     6781 gctagcgcac gggatgctgg aacttcttcc cgcagccacc ggcgctctgg cctggccggt
     6841 ggtcctggca ctggcctggg tggcgggcga gctcggccat cgctggctga accttccgcg
     6901 catcagcctg tatggcctga tcggcttcat tttcgggcat gcgcaactgg gcctgcttcc
     6961 cgctaccagc gatggcctgg gcgtcctggt agcgaacatc gctttcgggc tgatcctgtt
     7021 cgagttcggc taccgcatca atgtgcggtg gctgcgcacc aacccctggc tcggcgcgac
     7081 aggggtcctg gagacactgg ccacctttgt cgcggtgtac gcgctggcgc gctggtgggg
     7141 ctcgcccaac ctgacagcgc tgctgctggc ctcgctgtcg atgtccacat ccccggccgc
     7201 gctgatgcgc gtgatcaacg agcagcgcag ttccggccag gtcaccgaac ggatcctgca
     7261 cctggccgcg ctcaattgcg tgctggcggt tttcgccttc aaggtcatcg tcggcttctg
     7321 gaccttcgag agctcgggca acctcgggct ggcattcttc aacagcctgg tggtgttggc
     7381 ggtctccggc gccttgggtg ccatgttcgg ggtgggagtg cccgggctgc tgcgactgac
     7441 cgggcgcatg agccaggatg gcacggtggc gttcgcgatc gcggtgatcc tgctggtggg
     7501 gctgacccac gcgttcaagt tctccccgct ggtggcggcg ctcaccttcg gcctggtcgc
     7561 ccgcaaccgg cgcctcacgc tgacccagac gcagcgcaat ttcggcgccc tgggcgatct
     7621 gctggccgtg gtcctgttcg cctacgtggc cacccgcatc gaatggccgc gggtggcgac
     7681 cgggctcggc ctcgggctcg cgctggtggc cacgcggctt gccaccaaag tggctgccgt
     7741 cgcggcgctg gcgcgtgcca gcggcatctc ctcgcgcaag ggcggcctca caggcattgc
     7801 gctgatgccg atgtcggtgc tggtggtgct gctgctggag gagcacacgc gccatcttgg
     7861 catcgacctg gtggaccagc tggctccgct ggccgcggcg acgctgttgc tcgagctcat
     7921 cggcccggtc gccacacagc aggccttgcg cctcgcgggg gaagcgccgc aggcacggga
     7981 gcacgggacg ggacgctgat gccgctcgaa cacttcgcca cctccgagtc cctcactttg
     8041 ggagtcgagc tggagctcca gctggtaagc ctgagcgact tcgacctcat ggacgccagc
     8101 ccggacatgc tcgacctcct cgcgcggcgg cgctttccgg gcaacgtggt gcccgagatc
     8161 accgaaagca tgatcgagat ctcgaccgac gtgcacagag aacacgccgg cctgcttggc
     8221 cagctgcacg agatccggga tgcgctggtc gccgcgggcg acacgctgaa cgtgggcatc
     8281 tgcggcggcg gcacccatcc gttccagcta tggtccgaac gcaagatctt ctcgaagccg
     8341 agattcaagg aagtgtccgc gctgtacggc tacctcgcca agcagttcac gatcttcggc
     8401 cagcatgtcc acgtcggctg cacctcgggc gaccaggcga tgtacctgct gcacgccctg
     8461 aaccgctatg tgccgcactt catcgccctg tcggcgtcct cgccttacgt gcagcgcaac
     8521 gacacgctgt tcaattccgc gcggctgaac tcggtgttcg cctttccctt gagcggccgc
     8581 gcgccgttca cgctgcgctg gagcgagttc gagaacgact acttcgccca gatggaaagg
     8641 acgggcgtcg tcaagagcat gaaggacttc tactgggaca tccggcccaa gcccgagtac
     8701 ggaacgatcg agctgcgcgt gtgcgacacg cccttgacgg tggaccgcgc ggcggcgctg
     8761 gcctgttacc tgcaggcact ttgccggtca ttgctggccg gcagcgagcc ggcgccggcg
     8821 gaaagcgatt accttgtcta caactacaac cgcttccagg cctgccgctt cgggctggac
     8881 ggcaccatcg tgcatcccag gacatacgag gcgctgccgc tgcgcgagga catcctgacc
     8941 accttgcgca agatcgggga ccacgccgaa gcctgcgggg ccacggccgc cgtcgaccac
     9001 ctctacgaga cgatcaaagg cggtagcgac gcgagcctcc tgcgccggga gttcgagcaa
     9061 agcggcagcg cggagggaat ggtgaacgcc gccattcgcg ccttccgccg aggcggcagc
     9121 gggccggcac ggtagcccgc cggtctccgt ccagcagcag ggccggttca ggccgcgttg
     9181 gcgaggccgg cgcggctgct gaattcgtgg caggcggcct cgaagctgcg gatctcgaac
     9241 agcgcttcgg ccaggctcat ctcgcccttg tccatcagtt tgccggccag gtgttcggtg
     9301 agcggtgtca gccgcgccag cagcgccttg gtttcgccat agaggcgctc gctttgcgct
     9361 tccatgatgc cgcgcgtttc ctcgtcgagg ttgcggctgg caccgtggcc gtcgttcgac
     9421 agcgcgccga agttcagccg ggtcttgcgg tacatgccca tctccgccac ggcgtggtgc
     9481 agcagcttgg tcacgcgggt gatgtcgtta tgggcgccgt tggtcgtgcc ttcctcgcca
     9541 aagaacagtt cctcgttggc catgccgccc agcagcacgc gcacgtcgtg ctcgatgtcg
     9601 cccttggtct tgagcaggtt gctggcctgc ttgtggaaca cgaagcccag cgcgttgttg
     9661 cgcgggttgg ccttgagcga gatcttgatg gtcttcatgt cggcctggat cgcgtcccag
     9721 tcgctttcgg cccgcctgcg ctccagctcg aagtccacca ggaaatggcc ccattcgtgg
     9781 atggcgatga tgcgccgatc gcgctcgcgc tccttcgtgg tgttggcgtt gacgttgccg
     9841 accatcaccc gctcggccgc ccgcatcaag gtctcggcgc cgatcatgtc gccggtctgc
     9901 acggccgcga tgccggcctg gttgacgatg gtctccaggt ccgccgggct gcggccttcg
     9961 gtcacttcga ccagatggcg caggtccagg tcgggcagca ccttgtcggc gcgttgcgcg
    10021 aggtagtggc cgatgatcgc cagccgctct tccttgttgg gcatgcggaa gtcgaccttc
    10081 atctggaaac ggcgcagcat ggcctcgtcc atctgcatgg attcgctgtt gaagttgctg
    10141 gcgactatcc agatgatctc ggcctcgctc ttgctgcgca cgccgtccag cacggacagc
    10201 agggtgttct gcgtgtcgtc gtcgaacttg cggtggccgc cgcgcttcat gaacaggtcc
    10261 tgcgcctcgt ccaggaacac gatgcagcgc ttgcggcgct tggccatggc catgatgcgg
    10321 ctgagcgtgt tggagccgcc ggccacgaag ccggtttcca ggttggccgc cgagtggaac
    10381 aggatcggca ggttaagctc cttggccagg tagcttgcca gcttggtctt gccggtgccg
    10441 gccgggccgc tgaacatcac gttgaacggt ttgctgatgc cgtactcggc gtattcggca
    10501 cggcgctggt actggtcctt gatctgggcc acttcggcct tgatgtcgtc catgcccacc
    10561 aggtcgtcga tggatccgtg cacatgcgac ggttcgagga acttgagcga tttgaactgg
    10621 cctttgaggt ggaacgcgag gaagcccagc agccccagcg tcagcgcggc cgacagcacg
    10681 ccgctcacgc cggccttcca gccgtccttt tcggactgcg gacgcgcacc ttcgaaccgc
    10741 tggtccaggc gttggagcat ggcgacgcta tccttgtcca ggtcggcgcg cggcacgaag
    10801 gtcagtttgg cgccccagcc ggcggtgacg gccttgtccg cgaagatctt ggtttccatg
    10861 tcgctcgggt agtaggcgta ctgcttgccc tgcgattcga ccaggatgaa gcgctcggac
    10921 acgttccaca tcagcggccg gtagatgacc gtgatacgtt cgatagggtt acccgcgagg
    10981 aagctcagca ccgagccggt gccgaatacg acgggcagct tctcgttgaa caccgacacg
    11041 ccgccggccg gcaactcgcc gggtgtcatc gtcttgagct gctcggccac ggcggcctgc
    11101 ttctgcctga ggttgtacga atacagggcc gtcgatggaa tgagcaccgc cacgatgatg
    11161 atcatggcct tgacgaacac ggattgaatg acgaaccagt cctgcagtcg agccgcgccc
    11221 tttttcaagg tcagccacaa gttgctcgcg ggccgctcgc ctccggcggc ggcttcggga
    11281 gtgggcgatg agctgggcga taaatccgac atggttgtcc ttcgtgaccg aacgcctcgg
    11341 tcccgtttca ggcgaaacgg gtctctagca atactgaaaa attcggggag aagctcgtcc
    11401 ggcagggctg ccgcgggcct ggattgttgt tgcttttgtg ccgcttacac gttcgatcgc
    11461 cgggctgcgc ctcttgtcag cgcaatccta cagactcccg aatggatagg ctagcgctag
    11521 gcttatcgag gacattgcgg tcccgttgct cgtgacaaat tcacgcgctc cgcggcgggt
    11581 tgcgtgttgg ttgtgagaca tcatggtgcg ttccgttcag agtgggcaaa caggcttgaa
    11641 ctgaacgtta aggcgaccgc gtttggcgcc ttttgtgatt tgtcatttca aatcgcatca
    11701 aaatgcaact tcatgtattt tgtacgacgg caacgaccag gcgcaagctg gcccaggaac
    11761 caaggccgtg ctggcgactc caacgtaacg gccgggtttt cttcaagttt cttcccccgg
    11821 ctcttcaatc cctcggctca accccgaggg attttttctg cctcatccac gccgagccag
    11881 tccagggcgt cgctttccgc gtcgaacatg gcgatatcgg gacacatgga aagcccgtcg
    11941 ggcacgtcgt cggtgtcgac cagttcagtc gtcgccacca gcacgacctt ggtcttgtgc
    12001 gtcaacgccc gccgtagccg gtcgcagatg aggaacttgc cttccgcgtc gggcggcgtg
    12061 gccacgcggc gcaggtcgat cagcacgtgg tcctgccgca tccgcgcgac ggccgccttg
    12121 accatgtaag ccagcttcag tgcatcgccg ttgcgccagc ggccctcgac atcgaagcgc
    12181 atatgaacac tgtcatgacg ggaagcaact acgagattca tggcctttgc gccggtttgt
    12241 ggacggctaa gagtgccaca gccatttgta cgggctcgga tacaagcgca tcagtagtag
    12301 ccaagcgtga ctgccgcgct tgggcctgtt cacaggccct gatcctggtc gatgggactg
    12361 gccggcagcc aggcccatgc tggccgcacg cccggttgcc ccggcgcacc ggcgcctccc
    12421 ccgattgctt gcccatccga agatgtcagg cggccgggag cctggccctc gcgtccacgc
    12481 gcgcgagccg cgccagcagg tactccactt cctccctggc cctgggcgac atggccgacg
    12541 ccggcttgcg ctgcgcctcg gatgccaaca ggccgcggcg catcagcaca tatttgcgca
    12601 ccgccaggcc cgccccgggc tgctgttcgt agcgcagcag cggcaggtgc gcatcgaaca
    12661 ggtcgtgcgc ctggtcccgt cggccctgtg ccgacagccg caccagctcg acgagcatgt
    12721 ccggaaagca gtagcccgtc atcgcaccgt cggcgccgcg ctcgacctcg aaatcgagga
    12781 acaggccgcc gttgccgcac aggatcgaga tccgccgcat ctcgcccttg ttctcccact
    12841 cccgcaaggt ggagatcttt tccaggccgg gccagtcttc gtgcttgagc atcacgcatg
    12901 aagggttgtc gttgacgatg cggcggatga cggacggggt catcacgacg gtgaacgtca
    12961 gcgggtagtc ctggatcacg aagggaacgt cgggcccgat ggcggcgacc gcctgtgcgt
    13021 aatagccgac gatctggtcg tccgttcgca gcgtgttcgg gggcgcgatc atcacgccgg
    13081 cggcgcccgc atccatcgca tcgcgcgcca gcgcccgcat cgccgcgaag cccggcgcgg
    13141 agacacccac gatggtgggt gtggccgatc gctggatgaa gcgcgaggcg acttgcagcg
    13201 cctcggcctg ctcgagcttc ggggcttcgc ccagctggcc cagcaccgtg gtgccggcaa
    13261 cgccgctcgc ggcgtaaaag gccgccagcc ggtcgatgga ggccaggtcg acggccccgt
    13321 ccggacggaa gggcgtgggc gcgatgacat agacgccgcc ggcttgcgcg gtaatttgca
    13381 tggggacctc tctcgggaaa aacgcaacgg ctggcttgcg ctcgatttac gggtgccggc
    13441 cggctcgcga agccgctatc ttgcccggtt tcgcgctggc cggcgcaacg ggtcggacgg
    13501 ctgggggccc gagccattcc acgtcagtcg cccctggagt ccgtggcccg ggcgtcttta
    13561 ccggaagggc tacgacctgt tcgtgttatg agcgtgctta cccggcgtgc gcaaagacgg
    13621 cctgcaacgc gaagtgcgtg ggcagataca ggccgatcgg aaacgccagc atcaggaacg
    13681 cgagccagac gagcggatgg acgcgccgct gaaacctgtc cggcccgcgc acccagttga
    13741 tgttctcgtg cgccggcgtc gtcaccgcat aggtgagcgg cagcacgacc caggcgagca
    13801 gggtctggcc ggccagtgcg cgcgggtcgt agcccagctc gtacagcatc cagatcagca
    13861 cagccgggag cacaacgtgg aacagcgaca gtgcgcgcac gaagcgcggc ttgcgtgcat
    13921 cgaacatgta gccggcgaga tcggtgacgc gcagcccgaa cagcaggcgg ccgaaatagc
    13981 tggcgctcca caagagctcg ggcaacagca ccgccagcgc catcatgctc gcgagcaagg
    14041 cgctgtcgag ccacagcgcg acgccggtgg cgatcagcgc gatgtcggaa aaccagagaa
    14101 agtttcgcca gccccacacg acggcgtaaa cgggcaggat caccgcgagc tgcacggtgt
    14161 acgcgatctt gatccacagg gcgatctcca ctcgcgtcgc ctcggctatg ccacgaacac
    14221 cgtcttgata ttcacgaact ccttgtcgtc cacctcggcg cggagcatct cctcataagt
    14281 ctcgagcttc tcgccggtcg caggatcgat ggattcgatg accatgtcag tgcggagcct
    14341 cctctgaaat ccccctcacc ccaacccggc ccccttgctc gctctccccc tgcgggggcg
    14401 agggagccac aactgcttcc ccggcagaag gcggagctac tccctctccc gcttgcggga
    14461 gagggctggg gtgagggtgc gcccggaaca actcgtccgg cgcgaagtcg tcgacctgga
    14521 tcgccgcgcg cgcggcgtcc tgcgctgccc gcagctgcgc cgcttcgccc gcggtgagca
    14581 cacccgccgc cacggctgcg gcccagtccc ggccatggcc ggcatcggcc cccttgcgca
    14641 ccagaggcgc gacctcgtgc gccaggacga aggcgcgctc gacacgttcg atgccgtcgc
    14701 gccggcagcc gcggaagacg ccatgagtga tgcgctcgcg cgcggcgttg tgctgcatca
    14761 gcagctcggc gcaggcatgg gtcacccggt ccgtcgggcc acgctggcgc acgccccagg
    14821 gcaacgtcac cacccggagc aaggccgcga gcggccggtt cggaaagtta cggatgaatc
    14881 cgtcgagcgc cgtttcgatg cgtgacaggg cactgtcgca gcaccactgc acgagcacga
    14941 catcgtcgcc cgggcggccg tcgtcggcga aacgcttcag caccgcgctc gcgaagacca
    15001 actcggacag catgtcgccc agccgcgccg acagcgactc gcggcgcttc agcgctccgc
    15061 ccagcgtgag cagcgccacc tcgctggcca gggcgagggt cgcgcagtgg cgcgcgagct
    15121 gtcggtagta ccgcgtctcg ggcgcaccgc gccccgcgtg aggcgccgga ccgacccggc
    15181 ccgcggacca gctgcgcacg aaagcgcgaa ccagggtcgc ccccgcgtgg cgcaggtgct
    15241 gtgcagccgc atcggcgaaa tgctcgcgag cctcgtgctc gtcgggctcc tgcagcgcaa
    15301 gcatctcatc gagcagatgc gggtggcatc gcaccgcgcc ctggccgaag atgatcaggc
    15361 tgcgcgtcag tacgttggcg ccttccacgg tgatcgcgac cggcagcgcc cggtaggcac
    15421 cggccagata gttgagcggt ccgtccatga tggccttgcc gccatgcacg tccatcgcgt
    15481 cattgaccgc cacgcgcatg cgccatgtcg catgggcctt cacgatggcc gacacgatcg
    15541 ccggctcgct cccctgggcc agtgcgctgc aggtgaagcg ccgtaccgct tccagctcgt
    15601 acgcgatgcc cgcgatgcgg cccagacact cgcgaacacc ctcgaacttg ccgatcggca
    15661 cgccgaactg ctcgcgcacg aacgcatagg cgcccgtggc ggcggccgcg aggcaggcgc
    15721 tggcggctga cagcgagggc agcgaaatgc cgcgccccgc ggccagcgcg ctcatcagca
    15781 tcggccagcc ctggccgatt cgctcgcgcc cgccgattac ccagtccagc ggcacaaaga
    15841 catcggtgcc ggacgtcggg ccgttctgga acgcctgcat cgcgggcaga tggcggcgcc
    15901 cgacctgcac gcctcgcgtt cgcgtcggaa tgagcgccag cgtgatgccg agatcctcct
    15961 cgccgcccag cagatggtcg gggtcctcgg ccttgaaggc gagcccgagc agcgtggcca
    16021 ccggagccag cgtgatgtag cgcttggccc aggtgacgcg caggccgagc acacgccgtc
    16081 cctcgtgcac gccctcgcag acgacgccgc ggtcgcgcat cgcggcggcg tccgaaccgg
    16141 cctcttcgct ggtgagcgcg aagcagggaa tctcgcggcc gtcggccagc cgcggaagga
    16201 agtgctcttt ctgcgcatcg gtgccgaagc gcagcagcag ctcgcccggt ccgagcgagt
    16261 tgggcaccat gacggtgacg ccggcggtga tcgagcgcgt cgaaacgcgc ttcaccacct
    16321 cggagtgagc gtaggcggaa aagcccagtc caccgtagcg cttgggaata atcatgccga
    16381 agaacttatg acgcttcagg aaggcccagg cctcctgcgg cagatcgccc tcttcgctca
    16441 cgcgccaatc gtcgaccagg gcacagagct gctgtaccgg gccgtcgagg aatgcgcgct
    16501 cctcctcgct caagcgcggc ggcggcagcg acagcagttc attccagtcg ggatggcccg
    16561 aaaagagcgc cgcgtcccac cagacgtcgc cggcggcaag cgcctcgcgc tcggtggcgg
    16621 acagcgcagg cagggccttg cgggcccgat cgaacaacgg gcccgtgagc agcgtgcggg
    16681 cgggtctgcg catcatccga attcgcccgc ctgcgtggtc ggcgccttgc catcgcgctt
    16741 gtcgcgccgg gctcgctcga tggtctttct ctcgggcatg gtgcatctcc acgggtggat
    16801 ccccatggta ggacgagccg gggcgcaagg gggttcgtcg ttgcgcgtca gttacattcg
    16861 cagcggggcc gtcccagccc agtcacttgt ccagcggcag gcgccatgcg ctcggcagcg
    16921 gtacggcagg gctcggcgcc agctatcgcc aagccggttg cccatgcgtg gcctgctcat
    16981 gcgcggcgcg cgcctcctgc cgcagctcgt caacgctgcg cttctcgcgc aggcgccggt
    17041 ccaccagcac gcggaagaac cagggcacga agaagatcgc caggaaggtg gcggccagca
    17101 tgccgcccat cacgccggtc cccaccgaaa cgcgcgcccc ggcgccggcg ccggtcgaga
    17161 ccgcgagcgg cagcacgccg aggatgaagg cgagcgaggt catcacgatc ggccggaacc
    17221 gcaggcgtgc cgcctccagt gccgccgcgg gcgcgctcat accttcactc gccttgagcg
    17281 atgcgtactc gacgatcagg atcgcgttct tggccgcgag cccgagcagc gtcaccagtc
    17341 cgatctggaa atacacgtcg ttggtcatgc cgcgcagcca caccgccgcc agcgcgccga
    17401 aggtgccgaa aggcaaggcc agcagcaccg aaaggggcag cgaccatttc tcgtattgcg
    17461 ccgacaggat caggaacacc atcaccgcac ccagcgccag cgcgatcccc gaagtgccgc
    17521 tggagcgctt ctcctggaac gaggcgccgc cccagtcgta gctgaaatcg gccggcaagg
    17581 tcgccgcggc gatgcgctcc acctcggcaa tcgcctggcc cgagctgacg cctggcgcgc
    17641 cctgcccgaa cagcttgacc gccggcaggt tgttgaaccg gtccagcgat tcgggaccgg
    17701 cggaaaagcg cacgtcggcg atggcggaca ccggcaccat ctcgcccttg tccgagcgca
    17761 cccacatccg gccgatgtcg tcgggctggc gccgatacga gggatccgcc gacatcagca
    17821 cctgccaggt gcggccgtac ttgttgaagt cgttcacgta gtgggaaccc agcgtggccg
    17881 ccagcgtgtc gaagacgctg tcaaccggca cgccgagcgc cttcgcacgc gagcggtcca
    17941 cgtccacata cagctgcggc gtcgaagcgc gccacaaggt ctgcacgcct gcgagcaatt
    18001 tcgattgcgc caccgcgccc tggaacttct gcgtgacctc ggacaatttc gccgagccgc
    18061 cctcgccgcg gttctggatg tagaactcga agccgccggc gtttcccagg ccgaagatag
    18121 gcggcgggtt gaaggccagc accagcgcct ccttgagtcc cgccgtcttc atgaacagct
    18181 ctcccaccag cgcctgcacc ggcaccttgc gctcgtccca gtgtttctgc gtcacgaaga
    18241 tggtggcggc gttgttgcga aagcccccgc ccaggaagtc gaagcccgcg aacgagatcg
    18301 cgtccaggtt gttggggttg gacttgatca cctgcagcac gtcgttcacc accttgtcgg
    18361 tgcgctgcag cgacgcgccg tcgggcagga tcaccgcggc gatgtagaag ccctggtctt
    18421 cgtcggg
//
LOCUS       DASWIY010000062         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1894162, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000062 DASWIY010000000
VERSION     DASWIY010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894162"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..235
                     /locus_tag="VGA59_02400"
                     /pseudo
     CDS             68..235
                     /locus_tag="VGA59_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009932860.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(608..1984)
                     /locus_tag="VGA59_02405"
     CDS             complement(608..1984)
                     /locus_tag="VGA59_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HEX9718555.1"
                     /translation="MQIMQSMGRGLSSVSARYMPDPMIFAIVLTFITFILGIALTDSG
                     PMQMIIHWYGGFWSLLTFAMQMALILMTGYALALAPGLRDGIRWLARVPKTGGQAVFV
                     TALVATIFTWFHWGLGLIVAALFALTLAREAHKSGIKVHYPLLGAAAYTGQMVWHIGP
                     STSAGLVSATPGHFLEKVIGIVPITETAFTPYALLLSALLVFFVVPITMWLMTPKDSE
                     SQGLASYAPDKLEGAALPHEDSEVTAAKPGTRTPADWLENSRVIAGLIALMGIIYTIY
                     YFATKGLDLNLNIFNFAFLMVGLALHGTVIRYIRAIGQGAAGAAGIILQFPFYGGIAG
                     MIGASGLAKIMAGGLLALSTTENFPVVAWLTAGFVNIFVPSGGGEWAVIGEVISRTAM
                     ALDVPIGKAIIAYGAGDMWTNMLQPFWAIPLLGIMGLRARDIMGYTVGLMIIAAPFIY
                     IGLRYFPY"
BASE COUNT          463 a          790 c          814 g          443 t
ORIGIN      
        1 cgctgcgacc ggcgcccgaa ccgctcgctt ctcgcggcac cggcgcgaag gaattttcta
       61 tcctttcgag atcgtcacgg cgcgcggcct ggtgccgttc ctcgacctcg cctaccaggg
      121 cttcggcgat agcctcgaag ccgacgccca cgccgcgcgt cttttcgccg gcgccatgac
      181 gccggtgttc ctgtcgagct cgttctcgaa gtcgtactcg ctctacggcg agcgctagcg
      241 gccattgacg gatggcgcga taaactcacc ggtgacccaa ggaacttcat taggggtcat
      301 gactttgtgg aactgctcgc gtgggcgata ggaaagttca aggggctaaa gcaatttact
      361 tcagcggagg cgatcgaacg actctttgtg ctcattgccc cgagagcccc tggcttggcg
      421 ctgctattcc cttgaagaac ctcttcgcgt tcacgcaaac gtagggaaaa cgtagcgcca
      481 tccccgggtt tccccggggt ttggcgacta aggacgggtc cagaaacaac tagttggcgc
      541 ttctacctcc ccctgctaat ccgcaggtcc caggttcgag ccctggtcgg ggagccatcg
      601 cgttcgctca gtacgggaag tagcgcagcc cgatgtagat gaacggggct gcaatgatca
      661 tcaggccgac ggtgtagccc atgatgtcgc gggcgcgcag tcccatgatg cccagcagcg
      721 ggatggccca gaagggctgc agcatgttgg tccacatgtc gccggcgccg taggcgatga
      781 ttgccttgcc gatgggcacg tccagcgcca tggcagtgcg gctgatgact tcgccgatca
      841 ctgcccactc gccgccgccc gagggaacaa aaatgttcac gaagccggcc gtcagccagg
      901 cgacgacggg gaagttttcc gtcgtcgaca gggcgagcag cccgcctgcc atgattttgg
      961 cgaggccgga ggcaccgatc atgcccgcga tgccgccgta gaacgggaat tgcaggatga
     1021 tgcccgccgc tcccgccgcg ccctgcccga tggcgcggat gtaccggatc accgtgccgt
     1081 gcagcgccag gcccaccatc aggaaggcga agttgaagat gttgagattc aggtccagtc
     1141 ccttggtggc gaagtagtag atcgtgtaga tgatccccat gagagcgatg aggcccgcga
     1201 tgacgcggct gttctccagc cagtcggcgg gagtgcgcgt gcccggcttc gccgccgtga
     1261 cctcggaatc ttcgtgcggc agcgccgccc cttcgagctt gtccggggcg tagctcgcca
     1321 ggccctgcga ctccgagtcc ttgggcgtca tcagccacat ggtgatcggc accacgaaga
     1381 agaccagcag tgcgctcagg agcagggcgt agggcgtgaa tgcagtctcc gtgatcggca
     1441 cgatgccgat cactttctcc aggaagtggc ccggcgtggc cgagaccagg cctgccgagg
     1501 tcgagggccc gatgtgccag accatctggc ctgtgtacgc ggcggcgccc agcagcgggt
     1561 agtgcacctt gatgccgctc ttgtgcgcct cgcgcgcaag cgtgagggca aaaagggcgg
     1621 ccacgatcag gccgaggccc cagtggaacc aggtgaagat cgtggcaacc agcgccgtga
     1681 cgaataccgc ctggccgccg gtcttcggca cccgcgccag ccacctgatg ccgtcgcgca
     1741 gacccggagc gagcgccaaa gcgtagcccg tcatgagaat cagggccatc tgcatggcaa
     1801 aggtgagaag gctccagaag ccaccgtacc agtggatgat catctgcatc gggccgctgt
     1861 cggtgagggc gatgccgagg ataaaggtga tgaaagtcag cacgatggcg aaaatcatcg
     1921 ggtcaggcat gtagcgggct gacaccgacg acaagccgcg gcccatggat tgcatgattt
     1981 gcatggatcc tcccctctgg tgcttcgcgg gcggccttgt gggcccttct tatggtgcag
     2041 gcgaaacgta acgcatatcg caggggttct caagtaccgg agctccggca cgccatcgcc
     2101 gtcgtgctcg gacccggtca acgtggtgat tcacgagcat ttcggcgaga acctgccctg
     2161 cggcaccacg gtccaggtga gcggtcttga aggccccggg cgtctaccgg cacccggggt
     2221 tttcgccagt cgtgacgatc gaaaggccgc cgaagctggt cctcatcgcc ggccagacgc
     2281 cttcggacga gaactaccgc tgcgtggcgc ccggcgacct gcgccggcag tacctttttt
     2341 atttgccata agaccgcttg cgtgcggcaa gcccaagcca ttgctgctcg accgcggcct
     2401 cgagctgctc gccatgctga aggaagacat gagcagcatg ggggccgaag acatccacca
     2461 gctgcagcgc acgtgggagc tgcaccaccg ggtcctggcc tcggagtcgg
//
LOCUS       DASWIY010000063         2938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2624441, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000063 DASWIY010000000
VERSION     DASWIY010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2624441"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..701)
                     /locus_tag="VGA59_02410"
     CDS             complement(90..701)
                     /locus_tag="VGA59_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9718556.1"
                     /translation="MNSKRGHAKVYLVGCLAGLLTIALFVVAVVWAAERSGRPDYQRY
                     DLSRPWFDGKPWSWMLDMVNQPSIKPQQVGTIQQFPVNSVPRSGVEPEVPNVSNASGL
                     LRDQIPLNPMQATPASLANGRKIFNIYCAACHGQDGKGQTPVAAKGMPAVPIDALRLA
                     FSEPHLYNKIRYGGPIMPAYGFQTTHAERWNIVNYLKSADFGK"
     gene            complement(691..1218)
                     /locus_tag="VGA59_02415"
     CDS             complement(691..1218)
                     /locus_tag="VGA59_02415"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX9718557.1"
                     /translation="MRAPFKGIWASFAHVDEAAAVMQRLHTEGRKYSVLSPCPRHEFY
                     DAMGRPQSWLPWITLLFGGLGIFFGYGLPTWTALDWVLPVSQKPIVGIPAFTIIGFEL
                     MVLLGGISTAVAIVVLGHLELFRQRLPSSPAFKSYNRFSNDRFGVVVSCEEGDAAALE
                     QLMREHSAEEVVREF"
     gene            complement(1223..2638)
                     /gene="nrfD"
                     /locus_tag="VGA59_02420"
     CDS             complement(1223..2638)
                     /gene="nrfD"
                     /locus_tag="VGA59_02420"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX9718558.1"
                     /translation="MSAITYADVNADVLRTLERPKPAYFLLLIGFATALCIGVLSWAI
                     QIDVGMGLGGISHPVGWGVYITNFVFWIGIGHAGTLISAVFFLTRAPWRAPVYRAAEA
                     MTVFAVMTAGLFPLIHVGRVWFAYWLIPYPNQRMLWVNFKSPLLWDVFAVSTYLTISV
                     TFFIIGLVPDLASMRDSARGLRKLIYMSLAAGWNYSYQQWMHYARGYLILAAFATPLV
                     FSVHSIVSWDFAMSLLPGWHTTIFAPYFVAGAIFSGLAMVLTIVIPLRTVFDLKKYIT
                     DHVLQSLARVILFTSVIVGYAYATEFFIAYYSGVEFERALFYFRIFGQPGDGAPFFHK
                     GEMGFPQIAFFLMVFCNVISPLPLWRWKIRNNAVALFIISILINIGMWFERFNIVVSS
                     LQRDFNPANWGPYWPTPVEFGITLGSFGFFFTFFLLFVRAMPSLAIAEVKESLSPPYK
                     HANHDHVPWREYATERTEDKD"
     gene            complement(2640..>2938)
                     /locus_tag="VGA59_02425"
     CDS             complement(2640..>2938)
                     /locus_tag="VGA59_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HEX9718559.1"
                     /translation="KCTFCVQRINRARQTANAEGRNLRDGEVTPACVQTCPTKALTFG
                     NLADSNSEVSKKALRQRNQREQRVRQYEVFPELKQEPAITYLRKVTFEPIHQEA"
BASE COUNT          585 a          919 c          943 g          491 t
ORIGIN      
        1 acccagcgcc accagcagcc acagcagcat gcgccagccg gatgccaact gcaggttgga
       61 ttgctccaaa atcgctttca tcgagctcct cacttaccga agtctgcgct cttcaaatag
      121 ttcacgatgt tccagcgttc cgcgtgcgtg gtctggaagc cgtatgccgg catgatcggc
      181 ccgccgtagc ggattttgtt gtacaagtga ggctcggaga acgcgagccg cagcgcatcg
      241 atcggcaccg cgggcatgcc cttggcggcc acgggcgtct gccccttgcc atcctggccg
      301 tggcaggcgg cgcagtaaat gttgaatatc ttgcgcccat tggcgaggga ggcaggtgtg
      361 gcttgcatgg ggttgagcgg aatctggtcg cgcagcagtc cggaggcgtt gctcacgttg
      421 ggcacctcgg gctccacgcc gctgcgcggg acggaattga cagggaactg ttggatggtg
      481 cccacctgct gcggcttgat actgggctgg ttgaccatgt ccagcatcca cgaccacggc
      541 ttgccatcga accagggccg tgagaggtcg tagcgctggt aatccgggcg cccgctgcgc
      601 tcggcagccc acaccacggc caccacgaac agggcgatcg tcagcagtcc ggccaggcat
      661 ccgacgaggt agaccttggc atggcctcgc ttagaattca cgcaccacct cctcggcgga
      721 atgctcgcgc atcagctgtt ccagggctgc agcatcgcct tcctcgcagc tcaccaccac
      781 cccgaagcga tcgttggaaa agcggttgta gctcttgaac gccggcgagg agggcagccg
      841 ctgccgaaac aactccaggt gccccagcac cacgatcgcc acggccgtcg agatgccgcc
      901 cagcagcacc atcagctcga agccgatgat cgtgaaggcc ggaatgccca cgatcggctt
      961 ctggctgacc ggcaacaccc agtccagcgc cgtccaggtg ggcaggccat agccgaagaa
     1021 gatgcccagg ccgccgaaca gcagcgtgat ccagggcagc cagctctgtg gacgtcccat
     1081 cgcgtcgtag aactcgtggc gggggcaggg cgaaagcacg ctgtacttgc ggccttccgt
     1141 gtgcagccgc tgcatgacgg cggctgcctc gtccacatgg gcgaacgatg cccagatgcc
     1201 cttgaatggt gcgcgcatcg gtctagtcct tgtcttcggt ccgttcggtt gcgtattccc
     1261 gccacggcac gtggtcgtgg ttggcgtgct tgtacggcgg cgagagcgac tccttcacct
     1321 cggcgatggc cagcgagggc atggcccgca cgaagagcag gaagaacgtg aagaagaagc
     1381 cgaagctgcc cagcgtaatg ccgaactcaa cgggcgtcgg ccaatacggg ccccagttgg
     1441 ccggattgaa gtcgcgctgc agcgagctga ccacgatgtt gaagcgttcg aaccacatcc
     1501 cgatgttgat gaggatggaa atgatgaaca gtgcgacggc attgttgcgg attttccagc
     1561 gccacagcgg gaggggcgag atcacgttgc agaacaccat caggaagaag gcgatctgcg
     1621 ggaagcccat ctctcccttg tggaagaacg gggcgccgtc tcccggctgc ccgaagatgc
     1681 ggaagtagaa cagggcgcgc tcgaattcca cgccgctgta ataggcaatg aagaactcgg
     1741 tggcgtacgc gtaacccaca atgacggagg tgaacaggat cacccgcgcc aggctctgca
     1801 gcacgtggtc ggtgatgtac ttcttcagat cgaatacggt gcgcagcgga atgacgatgg
     1861 tgagcaccat tgccagcccg gaaaagatgg ccccagccac gaagtacggc gcgaagatcg
     1921 tcgtgtgcca gcccgggagc agggacatgg cgaagtccca ggacacgatg gagtgcaccg
     1981 agaacaccag cggcgtggcg aaggcagcca ggatcaggta gccgcgcgca tagtgcatcc
     2041 actgctggta ggagtaattc cagcccgccg ccaggctcat gtagatcagc ttgcgcaggc
     2101 cccgggcgct gtcgcgcatc gaggccagat ccggcaccag gccgatgatg aagaacgtca
     2161 ccgagatggt gaggtacgtg cttaccgcga acacgtccca cagcagaggc gacttgaagt
     2221 tcacccacag catgcgctga ttgggatacg gaatcagcca gtaggcgaac cacacgcgcc
     2281 cgacgtgaat cagcgggaac aggcccgccg tcatcacggc gaacaccgtc atcgcctcgg
     2341 ccgcgcgata caccggggcg cgccagggcg cccgggtgag gaagaacacc gcggagatca
     2401 gcgtgccggc gtgcccgatc ccgatccaga acacgaagtt ggtgatgtat acgccccagc
     2461 cgaccggatg gctgatcccg ccgagcccca tccccacgtc gatctggatc gcccagctca
     2521 gcaccccgat gcacagggcc gtggcgaatc cgatcagcag caggaagtac gccggcttcg
     2581 gacgctccag agtcctcagg acgtccgcat tgacatcggc ataagtgatc gcagacaact
     2641 cacgcctcct ggtggatggg ttcgaaggtc accttgcgca ggtacgtgat cgccggctcc
     2701 tgcttgagct ccgggaacac ctcgtattgc cgcacccgct gttcccgctg gttgcgctgg
     2761 cgcagggcct tcttcgagac ttcgctgttg gaatcggcca ggttgccgaa cgtgagcgcc
     2821 ttggtggggc aggtctgcac gcaggccgga gtcacctcgc cgtcgcgcag gttgcggccc
     2881 tcggcattgg cggtctgccg cgcccggttg atgcgctgca cgcagaacgt gcatttct
//
LOCUS       DASWIY010000064         5029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3003044, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000064 DASWIY010000000
VERSION     DASWIY010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3003044"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VGA59_02430"
     CDS             complement(<1..444)
                     /locus_tag="VGA59_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX9718560.1"
                     /translation="MAGVQAFYATHFALPLPPGHRFPMGKYQMLRDRLAAELPAVELM
                     QALPASDGELALAHSPAYIRAISDGSVDPKVMREIGFPWSPPMAERARRSVGATIAAC
                     RNALRDGISANIAGGTHHSYADKGGGFCVFNDAAVASRLMQAEHAR"
     gene            646..1194
                     /locus_tag="VGA59_02435"
     CDS             646..1194
                     /locus_tag="VGA59_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX9718561.1"
                     /translation="MLTAEQVLASQKATIETLFGLTGKAFEGVEKLVELNVTASKAAL
                     ADAAGTTQAILGVKDAQELLALQASLFQPLAEKTAAYSRHLYDIATGAGAEFGRTFEA
                     QAAETQKKFLAVVDNAAKNAPAGSETAVAVFKSAVAAGNNALESVQKAVKQATEVAEA
                     NFNAVANQAVNAAKTTTAAKKR"
     gene            complement(1437..2366)
                     /locus_tag="VGA59_02440"
     CDS             complement(1437..2366)
                     /locus_tag="VGA59_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9718562.1"
                     /translation="MIKQTRIIRNCSYAGTWLIALGIAALLAACASAPPQGEPVAAPP
                     QPIAKRPPRIGLALGGGAARGFAHVGVIQVLEEAGVRPDLVAGTSAGSLVAALYASGR
                     NGRQLQQVAETMEEAAFTDWTLPIFSRGVLRGEALARYVNAHVGGKPIEQMPIALGIV
                     ATDLNSGLGVLFQRGDIGTAVRASSAVPALFLPVKIGNQEYVDGGLVSPTPVRYVRQM
                     GAELVIAVDISSAPEGNQAGDPIEILLQTFAIMGKSINSYELRDADVVVRPALAGVGG
                     ADVAARRRAIDAGRAAMQRLLPQLKAAIEAKSK"
     gene            2410..3450
                     /locus_tag="VGA59_02445"
     CDS             2410..3450
                     /locus_tag="VGA59_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9718563.1"
                     /translation="MLIKALGSASILLALSAGASAQEQVLNLYSARHYSTDEALYANF
                     TRATGIKISRVDADDAGILSRLKAEGSASPADVILLVDAARLHKGEVDGLFQPIKSKL
                     LEDAIPVQYRGKPTPEGTPWFGFSTRARVIVYDKQKVKREDVDTYEELADPKNKGKVC
                     IRSGSHPYNLSLFGAVTEHLGEQKAEAWLKGVVANLARDPKGGDTDQIKAVGSGECQI
                     AVSNSYYIARLIRSTKPEDQALMQRVSVVFPNQSSWGTHLNIAGGAVARHARNPANAI
                     RFLEYLASPEAQNYFANGNNEWPVARGVKVANPALAAMGGGDFKSETIPLAAVAANQV
                     KVQQMLDRVGFR"
     gene            3701..4459
                     /locus_tag="VGA59_02450"
     CDS             3701..4459
                     /locus_tag="VGA59_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX9718564.1"
                     /translation="MEIKGKVFIVTGGASGLGEGTARMLAANGGKVVIADMQAEKGEA
                     IAKEIGGVFVKCDVSQEADGQAVVAKAASLGKLMGLVNCAGIAPAEKTVGKNGAHSLP
                     VFSKTIMVNLVGSFNMIRLAAEAMQKNEPESTTERGCMISTASVAAYDGQIGQAAYSA
                     SKGGIVGMTLPIARDLARNGIRNMTIAPGIFGTPMMFGMPKDVQDALAASVPFPSRLG
                     TPEDYAKLARHIFENEMLNGEVIRLDGAIRLAPR"
     gene            4510..>5029
                     /locus_tag="VGA59_02455"
     CDS             4510..>5029
                     /locus_tag="VGA59_02455"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX9718565.1"
                     /translation="MKLKLTLLATSALLAACATAPSNFSYAPPLLPEAPSGYAEKPGW
                     ATTRFAVAAANPLATDAGYQVLRAGGSAIDAAIAVQMVLTLVEPQSSGIGGGAFLLHF
                     NGKEVEAFDGRETAPAATDEKLFLGADGKPLAFYDAVVGGRSVGVPGTVRMLEMAHRQ
                     YGKLPWAKLFEPA"
BASE COUNT          948 a         1684 c         1575 g          822 t
ORIGIN      
        1 acgtgcgtgc tcggcctgca tcaggcgcga ggctaccgcg gcgtcgttga aaacgcaaaa
       61 acccccgccc ttgtcggcgt acgaatgatg cgtgccgcct gcgatgttgg ccgagatacc
      121 gtccctcaag gcattccggc acgcggcaat ggtcgcgccg accgagcgcc gggcacgttc
      181 ggccatgggc gggctccagg ggaaaccgat ctcacgcatg accttggggt cgaccgagcc
      241 gtcggagatc gcgcgaatgt aggccgggct gtgagccaac gccagctcgc cgtcactggc
      301 cggcaaggct tgcatgagtt caaccgcagg cagctccgcc gccagccggt cgcgcagcat
      361 ctggtacttg cccatcggga accggtgccc gggcggcagg ggcagcgcga agtgggtggc
      421 atagaaggct tgcaccccgg ccattgtgtc gcgcctgtgc aaccgggccg ttctagagga
      481 ttctttctag attgttgcga tgcaacaaaa aagtacttgc aataggccgg catgcctcta
      541 tactttgatc catgctgcac tgcaacaaaa cgcaggccct ccccggccga ggttcagagc
      601 agttatcgat cctgatccgc accttaacta actggagaat ctcatatgtt gaccgctgag
      661 caagtcctgg cctcccaaaa agccaccatc gaaaccctgt ttggcctgac cggcaaggcc
      721 ttcgaaggtg ttgaaaagct ggtcgagctg aacgtaaccg cctccaaggc ggccctggcc
      781 gatgccgccg gcaccacgca agccatcttg ggcgtcaagg acgcgcaaga gctgctggcc
      841 ctgcaagctt cgctgttcca gcccttggcc gagaagaccg ccgcctacag ccgccacctc
      901 tacgacatcg cgaccggcgc cggcgccgag ttcggcagga ccttcgaagc gcaagctgcc
      961 gaaacccaga agaaattcct ggccgtggtg gacaacgccg ccaagaacgc gccggccggc
     1021 tccgaaaccg ctgtcgccgt gttcaagagc gccgtggctg ccggcaacaa cgccctggaa
     1081 tccgtgcaga aggccgtgaa gcaggcgacc gaagtcgctg aggcgaattt caacgccgtc
     1141 gccaatcaag ccgtgaacgc cgccaagacc accaccgccg cgaagaagcg ctgagtctcg
     1201 tatcggaggt cccgcctccg agtcgattga ggcgggaact ctacaaatag cggagcatct
     1261 atccgacact cttgtggtga acaggagttc ctcaggttgt ctcctcgggt cctttcgaca
     1321 ccctcacggg ttcagggatc cttagcccca gtcgcaagac tggggctttt ttattgcgcc
     1381 ccatgggggc gagggagcga agcgacgctc gccccagtcg caagactggg gcttttttat
     1441 ttcgactttg cctcgatcgc cgccttgagt tgcggcagca gccgctgcat ggccgcgcgg
     1501 ccggcatcga tggcccggcg ccgggcggcg acgtcggcgc cgcccacgcc ggccagcgcc
     1561 ggccgcacca ccacatcggc atcgcgcagt tcatagctgt tgatgctctt gcccatgatc
     1621 gcgaaggtct gcagcaggat ctcgatcgga tcgccggcct ggttgccctc gggcgcgctg
     1681 gagatgtcca cggcgatcac cagctcggcg cccatctggc gtacatagcg caccggtgtc
     1741 ggcgacacga ggcctccatc aacatattcc tggttgccga tcttcaccgg caggaacagc
     1801 gcgggcaccg cgctggaagc ccgcaccgcc gtgccgatat cgccgcgctg gaacaacacc
     1861 ccgaggccgc tgttcaggtc ggtcgcgacg atgcccaacg caatgggcat ctgctcgatc
     1921 ggtttgccgc cgacatgggc gttgacgtaa cgcgccaggg cttcgccccg cagcacgccc
     1981 cggctgaaga tcgggagcgt ccagtcggtg aaggcggcct cctccatggt ctcggcgacc
     2041 tgctgcaatt gccggccatt gcgcccgctt gcgtacagcg cggcgaccag gctgccggcg
     2101 gaagtgcccg ccacgaggtc gggccgcacg cccgcctcct ccaggacctg gatcacgccc
     2161 acatgggcga acccgcgggc ggcgccaccg cccagcgcca gcccgatcct gggtggccgc
     2221 ttggcgatcg gctggggcgg cgccgcaacg ggttcgccct gcgggggagc cgaagcgcaa
     2281 gccgcaagca acgcggcaat gcccagggcg atcagccatg taccggcgta actacaattg
     2341 cgaatgattc gtgtttgctt tatcattgca ccttcattgt tcaacaacat cgctaggaaa
     2401 ctattcccca tgctgatcaa agctcttggc tctgcctcca ttttgctggc gctgtccgcc
     2461 ggcgcttccg cccaggagca ggtgctcaac ctgtattcgg cccgccacta ttccacggac
     2521 gaggcgctgt acgccaactt caccagggcg acgggcatca agatcagtcg tgtcgatgcc
     2581 gacgacgccg gtattctctc ccggctcaag gccgagggca gcgcctcgcc ggccgatgtg
     2641 attttgctgg tggacgccgc ccgcctgcac aagggcgagg tcgacggcct gttccagccc
     2701 atcaagtcca agttgctgga ggacgccatc ccggtgcaat accgcggcaa gcccacgccc
     2761 gaaggcacgc cctggttcgg cttctccacc cgtgcccgcg tgatcgtgta cgacaagcag
     2821 aaggtcaagc gcgaagatgt ggacacctat gaagaactcg ccgatccgaa gaacaagggc
     2881 aaagtctgca tccgttccgg ttcgcacccg tacaacctgt cgctgttcgg cgccgtgacc
     2941 gagcatctgg gcgagcagaa ggccgaggcc tggctcaagg gcgtggtcgc caacctggcg
     3001 cgcgatccca agggcggcga caccgaccag atcaaggccg tcggatccgg cgaatgccag
     3061 atcgcggtga gcaacagcta ttacatcgcc cgcctgatcc gctcgaccaa gcccgaggac
     3121 caggccttga tgcagcgcgt cagcgtggtt ttcccgaacc agagttcctg gggcacgcac
     3181 ttgaatatcg ccggcggcgc cgtggccagg catgccagga atcccgccaa cgcgatcagg
     3241 tttctggagt acctggccag cccggaagcg cagaactatt tcgccaacgg caacaacgaa
     3301 tggccggtgg cccgcggcgt gaaggtcgcc aaccccgccc tggccgccat gggaggcgga
     3361 gacttcaaaa gcgagaccat tccgctggcc gccgtcgccg cgaatcaggt caaggtgcag
     3421 cagatgctgg accgcgtcgg gttcagatag gaacaaaggg gacgcgaaga gccgctttgt
     3481 gcccggctct ttcgacggga cctgaaggcc ggtgggcgac atgcaagaga ccaactcggc
     3541 cggcatcgct cgaggtgaaa gcccgccccc ttccaggggg tcgggctttc atcttccggc
     3601 aagttgacgc gccgccaggt cgcgtcgaac gcgccgaatt atcgcggcgc ttcgcgtccg
     3661 cgataatgac gtttacgtaa acgtcaaaca ggagcactcc atggaaatca aaggcaaggt
     3721 cttcatcgtc accggcgggg cctccggcct cggcgaaggc acggcgcgca tgctggccgc
     3781 caatggcggc aaggtggtca tcgccgacat gcaggctgaa aagggcgaag ccatcgcgaa
     3841 ggaaatcggc ggcgtcttcg tcaagtgcga cgtcagccag gaagcagacg gccaggccgt
     3901 ggtggccaag gcggcgtcgc ttggcaagct gatggggctt gtcaactgcg ccggcatcgc
     3961 cccggccgag aaaaccgtag gcaagaacgg cgcgcattcg cttcctgtgt tctcgaagac
     4021 gatcatggtg aacctggtcg gcagcttcaa catgatccgg ctcgccgccg aggccatgca
     4081 aaagaacgag ccggagagca ccaccgaacg cggctgcatg atttccacgg cttcggtggc
     4141 cgcgtacgac ggacagatcg gccaggccgc ctacagtgcc tccaagggcg gcattgtcgg
     4201 catgaccttg cctatcgcgc gcgacctcgc acgcaacggc atccgcaaca tgaccatcgc
     4261 gcccggcatc ttcggcaccc cgatgatgtt cggcatgccc aaggacgtgc aggacgccct
     4321 ggccgccagc gtgcctttcc cttcccgcct cggcacgccg gaggattacg ccaagctggc
     4381 ccgccacatc ttcgagaatg aaatgctcaa cggcgaggtg atccggctcg acggcgcgat
     4441 ccgactggcg ccccgctgat ccgcggtaat ccggattaat atgcatctcc ctcgaccagg
     4501 gagacgcgaa tgaaactcaa gctgacgctg ttggccacct cggcgctgct ggccgcttgc
     4561 gccacggcgc cttccaattt cagctacgcc cctcccctgc tcccggaggc cccatcgggc
     4621 tacgccgaaa agcccggctg ggcgaccacg cgcttcgcgg ttgcggccgc caacccgctg
     4681 gcgaccgacg ccggctacca ggtgctcagg gccggcggat cggcgatcga cgcggcgatc
     4741 gctgtgcaga tggttctgac gctggtcgaa ccccagtcca gcgggatagg cggcggcgcg
     4801 ttcctcttgc acttcaatgg caaggaagtg gaagccttcg atggccgcga gaccgcaccg
     4861 gccgccactg acgagaaact attcctgggc gccgacggca agcccctcgc gttctacgac
     4921 gcggtggtgg gcggccgttc ggtcggcgtg cccgggacgg tgcgcatgct cgagatggcc
     4981 caccggcaat acggcaagct gccctgggcc aagctgttcg agcccgcca
//
LOCUS       DASWIY010000065         6833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3366736, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000065 DASWIY010000000
VERSION     DASWIY010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3366736"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /gene="atpG"
                     /locus_tag="VGA59_02460"
     CDS             <1..866
                     /gene="atpG"
                     /locus_tag="VGA59_02460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX9718566.1"
                     /translation="AVGKEIRGKIKSVENTKKITKAMEMVAASKMRKAQERMRHARPY
                     SDKVRNIAANLGKANPEYTHPFMKVNDAKAVGFIVVSTDKGLCGGMNTNVLRAVTAKL
                     RDTQAAGNAIQAVAIGGKGFGFLNRIGAPVVSHVTQLGDTPHLEKLIGPVKVLLDQYA
                     EGKVSAVYLSYTRFINTMKQEPVVERILPLTDESFKADEGQHGWDYIYEPDAQSVIDE
                     LLVRYVEALVYQAVAENMASEQSARMVAMKSATDNAGNVIAELTLIYNKTRQAAITKE
                     LSEIVAGAAAV"
     gene            910..2337
                     /gene="atpD"
                     /locus_tag="VGA59_02465"
     CDS             910..2337
                     /gene="atpD"
                     /locus_tag="VGA59_02465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX9718567.1"
                     /translation="MAQANPQTNGNVQGKIVQCIGAVVDVEFPRDKMPKVYDALKMEG
                     TALTLEVQQQLGDGIVRTIALGSSDGLRRGNMVYNTNASITVPVGKATLGRIMDVLGN
                     PIDERGPIDTTLMASIHRKAPTYDELSPSQELLETGIKVIDLICPFAKGGKVGLFGGA
                     GVGKTVNMMELINNIAKAHSGLSVFAGVGERTREGNDFYHEMMDSGVVNKDNLGESKV
                     AMVYGQMNEPPGNRLRVGLTGLTIAESFRDEGRDVLFFVDNIYRFTLAGTEVSALLGR
                     MPSAVGYQPTLAEEMGRLQERITSTKVGSITSIQAVYVPADDLTDPSPATTFAHLDAT
                     VVLSRDIASLGIYPAVDPLDSTSRQVDPNVVGEDHYNTTRAVQGTLQRYKELRDIIAI
                     LGMDELSPEDKLAVARARKIQRFLSQPFHVAEVFTGTEGKYVPLSETIRGFKMIVNGE
                     CDHVPEQAFYMVGGIDEAFEKAKKV"
     gene            2456..2872
                     /locus_tag="VGA59_02470"
     CDS             2456..2872
                     /locus_tag="VGA59_02470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX9718568.1"
                     /translation="MNTIHVDVVSAEESIFSGEARFVALPGEAGELGIYPRHTPLITR
                     IKPGSVRIEKADGSEEFVFVAGGVLEVQPNCVTVLSDTAIRGKDLDEEKANEAKAAAE
                     EALKNAKSDIDLAKAQSELAVMAAQIAALRKYRQKK"
     gene            complement(3233..3760)
                     /locus_tag="VGA59_02475"
     CDS             complement(3233..3760)
                     /locus_tag="VGA59_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX9718569.1"
                     /translation="MADKRGWPKRFARMLRHLWLDESDTRKAIPSELAQRLARRVAAS
                     EQRHSGEVRLYVEAGLPISYLWRDATPRQRALAMFGKLRVWDTELNNGVLIYLLLAEH
                     AIEIVADRGLSSKVPPQEWQAIVARMGTAFHEGRFEDGLTQALEEVSALLVAHFPAQA
                     GAVNANELPDEPVLR"
     gene            complement(3760..4656)
                     /locus_tag="VGA59_02480"
     CDS             complement(3760..4656)
                     /locus_tag="VGA59_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX9718570.1"
                     /translation="MRKAVLILWALLAWATLAWAQNLQPVPPLSAHVIDQTGTLDAIQ
                     LKGLEDKLAAFEASKGSQIAFLMVATTQPEDIASYANRVGNEWKIGRKGVGDGLLLVV
                     AKDDRKVRIEVAKTLEGAIPDLAAKHIIDEAITPNFRKGDFAGGLHAAADRIIARVTG
                     EALPEPEQPAQRGGGGEGFNWMDLAIFLFIAVPVVARVLRGALGKKLGALATGAGVGT
                     IALLVTSSIVVAAIAAFVALVVSMASGALPAMGSGRRGGWGGGPWTGGGGGGWSGGGG
                     GGGWGGSGGGGDFGGGGASGEW"
     gene            complement(4941..5531)
                     /locus_tag="VGA59_02485"
     CDS             complement(4941..5531)
                     /locus_tag="VGA59_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX9718571.1"
                     /translation="MKRLLLMILAALTLTGCGYNDFQRLDEQTKSAWSEVLNQYQRRA
                     DLVPNLVNTVKGEASFEQDTLSKVVEARAKATSMQVTPETINNPEAFAKFQQAQGELS
                     SALSRLMVVVERYPDLKANQSFRELRVQLEGTENRITVARNRYIQAVQQYNVLARSFP
                     TNLTAMVFNYDPKPTFTVQNEAQISVPPQVDFGAKK"
     gene            5577..6320
                     /locus_tag="VGA59_02490"
     CDS             5577..6320
                     /locus_tag="VGA59_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718572.1"
                     /translation="MRSGPIQVLQAAQRPGSPERKPRLLIAGATGALGNEVLRRLVGL
                     GRFESTQVLAREPITPALRGVTATVVSSGSPSQWMPTSAEAGVILFDPPRLFHDRERA
                     LWTPGPDQLPDVAQWMRRSGVQTLAVVLPHAQGTLPEALKRGLASLDEQAVAALGFER
                     LLLIRSARKPGAVNTRELLPRLAHWMLSVARFMVPSSEQPVRASKVAELVAVALQLAP
                     PGIHIAAPELVWRAAQGDLRETARQWLHF"
     gene            6352..6801
                     /locus_tag="VGA59_02495"
     CDS             6352..6801
                     /locus_tag="VGA59_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9718573.1"
                     /translation="MQKSKLQAANLEGNADDVEAAFYDALQNGDIDRLMACWADEDDI
                     VCIHPGGPRLVGPVAIRAAFESMFTNGSIRAWPERARKIQAVASAVHNVLERIEVLTP
                     EGPGQAWVIATNVYHRTAQGWRMVAHHASPGTDSDIQEVSGDPQVLH"
BASE COUNT         1284 a         2258 c         2185 g         1106 t
ORIGIN      
        1 tggctgtagg taaagagatt cgcggcaaga tcaagtcggt ggagaacacc aagaagatca
       61 ccaaggcgat ggaaatggtg gccgcgtcca agatgcgcaa ggcgcaggag cgcatgcgcc
      121 atgcccgccc gtacagtgac aaggtgcgca acatcgcggc caacctcggc aaggccaacc
      181 ccgagtacac gcatccgttc atgaaagtga acgacgccaa ggccgtcggc ttcatcgtgg
      241 tcagcaccga caagggcctg tgcggcggca tgaacaccaa cgtactgcga gccgtgacgg
      301 ccaagttgcg ggacacgcag gccgccggga acgccatcca ggccgtggcg atcggcggca
      361 agggcttcgg cttcctcaat cgcatcggcg ccccggtggt ctcgcacgtc acgcagttgg
      421 gcgacacgcc gcacctggaa aagttgatcg gcccggtgaa ggtgctgctg gaccagtacg
      481 ccgagggcaa ggtcagcgcc gtctacctga gttacacccg tttcatcaac acgatgaagc
      541 aggagccggt ggtggaacgg attctgccgc tgacggacga atcgttcaag gccgacgagg
      601 gccagcacgg ctgggactac atctacgagc cggatgcgca gagcgtcatc gacgagctgc
      661 tcgtgcgcta cgtcgaagcg ctggtctacc aggccgtggc cgagaacatg gcgtccgaac
      721 agtcggcgcg gatggtggcg atgaaatccg ccaccgacaa cgccggcaac gtgattgccg
      781 aactcacgct gatctacaac aagacaaggc aagccgcgat cacgaaagag ctttcggaga
      841 tcgtggcggg cgccgcggcg gtctgacgcc gcgcgccgac accgttcaat ttttattgga
      901 gcaacaaaga tggctcaagc gaatccccag acgaacggca acgttcaggg aaagattgtt
      961 caatgcatcg gcgccgtggt ggacgtggag ttcccgcgcg acaagatgcc gaaggtatat
     1021 gacgcgctga agatggaagg cacggccctt acgctggaag tgcagcagca gctcggggac
     1081 ggcatcgtgc gcacgatcgc gctgggttcc tccgacgggc tgcgccgcgg caacatggtc
     1141 tacaacacga atgcctcgat cacggtgccg gtcggcaagg ccaccctggg ccgcatcatg
     1201 gacgtgctgg gcaacccgat cgacgagcgc ggcccgatcg acacgacgct gatggcttcg
     1261 atccaccgca aggcgcccac gtatgacgag ctgtcgccgt cgcaggaact gctggaaaca
     1321 ggcatcaagg tgatcgacct gatctgcccg ttcgccaagg gcggcaaggt gggcctgttc
     1381 ggcggcgccg gcgtcggcaa gaccgtgaac atgatggaac tgatcaacaa catcgccaag
     1441 gcgcactcgg gcctgtcggt gttcgccggc gtgggcgagc gcacccgcga aggcaacgac
     1501 ttctatcacg agatgatgga ttcgggcgtc gtcaacaagg acaacctggg cgagtcgaaa
     1561 gtggccatgg tgtacgggca gatgaacgag ccgccgggca accgcctgcg cgtgggcttg
     1621 accggcctga ccatcgccga atcgttccgt gacgaaggcc gcgacgtgct gttcttcgtg
     1681 gacaacatct accgcttcac gctggccggc accgaagtgt cggcgctgtt ggggcgcatg
     1741 ccgtcggccg tgggttacca gccgacgctg gccgaggaaa tgggccgcct gcaggagcgc
     1801 atcacctcca ccaaggtggg ctcgatcacg tcgatccagg ccgtgtatgt gccggccgac
     1861 gatttgaccg acccgtcgcc cgccaccact ttcgctcacc tggacgccac ggtggtgttg
     1921 agccgcgaca ttgcatcgct gggcatctac cccgcggtgg acccgctgga ctccaccagc
     1981 cgccaggtcg acccgaacgt tgtcggggag gaccactaca acacgacccg cgcggtgcag
     2041 ggcacgctgc agcgctacaa ggaactgcgc gacatcatcg cgatcctggg catggacgaa
     2101 ctctcgcccg aagacaagct ggccgtggcg cgtgcgcgca agatccagcg cttcctgtcg
     2161 cagccgttcc acgtggccga agtgttcacc ggcaccgagg gcaagtacgt gcccctgtct
     2221 gaaaccatcc gcggcttcaa gatgatcgtc aacggggaat gcgaccacgt gccggagcag
     2281 gcgttctaca tggtcggcgg catcgacgaa gcgttcgaga aagcgaagaa agtttgacca
     2341 cccccgaagc ggcacacttc gtgtcccgcc tccccctcaa ggggacaaca ccagcagccc
     2401 ggcaaagccg gttctgcggt gtttcgcgaa aaagccaggt tccaggagat ttggaatgaa
     2461 cacaattcac gtcgatgtcg tcagtgccga ggagtcgatc ttctcgggcg aggcgcgttt
     2521 cgtggccttg cccggcgagg ccggcgaact gggcatctac ccgcgccata cgccgctgat
     2581 cacgcgtatc aagcccggct cggtgcgcat cgagaaggcc gacggcagcg aggagttcgt
     2641 cttcgtcgcc ggcggcgtgc tcgaagtgca gcccaactgc gtcacggtgc tgtccgatac
     2701 cgccattcgc ggcaaggacc tcgatgaaga aaaagccaat gaagcaaagg ccgccgccga
     2761 ggaagcgctg aaaaacgcca agagcgacat cgacctggcc aaggcccagt ccgagctcgc
     2821 cgtcatggcg gcgcagatcg ccgcgctgcg gaagtaccgg caaaagaagt aactaccttg
     2881 gggacagatc tttagctctg tccccaactg atcaagcttt gtgggacaga ccagaggagc
     2941 ggagcgatct gctctgtccc cgaccgatca agagcgggac agtccggaaa agcggagcga
     3001 tctgtcgccg actgaataca caaagtttga gcccggacaa aaagaagtca cgctgtaccc
     3061 tcgtcactgc agaatcctcg cgggacggag caaaatagcc gggaccaaca atacgaagca
     3121 agcggccctc gccgcacctg cgggcaaccg ccctcaaaac ccttgtgtgc aaacgcaggg
     3181 gtttttgttt ttggcttcaa tccgctgaat cgggcggcgc gcgcaagcct gcctatcgga
     3241 gcaccggctc gtccggcagt tcgttggcat tgaccgcgcc ggcctgcgcg gggaaatgcg
     3301 ccaccagcag ggccgagact tcttccagcg cctgcgtcag tccgtcctcg aagcggcctt
     3361 cgtggaaggc ggtacccatc cgggcgacga tggcctgcca ctcctgcggc ggcaccttgc
     3421 tgctcaggcc gcggtcggcc acgatctcga tcgcgtgctc ggccagcagc aggtagatca
     3481 gcacgccgtt gttcagctcg gtatcccaca cgcgcagttt gccgaacatc gccagtgcgc
     3541 gttggcgtgg cgtggcatcg cgccacaggt agctgatcgg caggccggcc tcgacgtaca
     3601 ggcgaacctc gccgctatgc cgttgctcgc tcgccgccac gcggcgggcg agccgctgcg
     3661 ccaactcgga cgggatggcc ttgcgggtgt ccgattcgtc cagccagaga tggcgcagca
     3721 tgcgcgcgaa tcttttcggc cagccccgtt tgtctgccat caccattccc ccgaagcgcc
     3781 gccgccgccg aaatcgccgc cgccgcccga accgccccag ccgccgccac cgccaccgcc
     3841 actccaccca ccgccgccgc ccccggtcca tggcccgccg ccccagccgc cgcggcggcc
     3901 gcttcccatg gctggaagtg cgccggaggc catggaaacc accagtgcga cgaaggcggc
     3961 gatggcagcg accacgatgc tggacgtgac cagcaaggcg atcgttccga cgccggcgcc
     4021 ggtggccagg gcgcccagtt ttttgcccag tgcacccctg agcacccgtg ccacgaccgg
     4081 caccgcgatg aaaaggaaaa tggccaggtc catccagttg aagccctcgc caccgccgcc
     4141 gcgctgtgcc ggctgctcgg gctcgggcag cgcctcgccg gtcacgcgcg cgatgatccg
     4201 atcggccgcg gcatgcaagc cgcccgcgaa gtcgcctttg cggaagttgg gcgtgatggc
     4261 ctcgtcgatg atgtgcttgg ccgccaggtc gggaatcgcg ccttccaggg tcttggccac
     4321 ttcgatacgc accttgcggt cgtccttggc cacgaccagc agcaggccgt caccgacgcc
     4381 tttgcgcccg atcttccact cgttgccaac gcggttggcg taactggcga tgtcctcggg
     4441 ctgcgtggtc gccaccatca ggaaggcgat ctgcgagccc ttgctcgcct cgaacgcggc
     4501 gagcttgtcc tccaggcctt tgagctggat cgcgtccagc gtgccggtct ggtcgatcac
     4561 gtgggccgaa agcggcggca ccggctgcag gttctgggcc caggccagcg tggcccaggc
     4621 caaaagcgcc cacaggatca ggacagcctt cctcacagcc tgtcatccca cgcttgagcc
     4681 gcgatccata gtggctcacg cagagcgccg ctccagtgac atggatgccg ggtcaagccc
     4741 ggcatgacaa ctctcatcaa atctccggac agcagtgggc ttgaccccgc actgacaaga
     4801 taccagtcgc cccgggcttg actccgcact gacaagatcc ctgtcgtctc gggcttgacc
     4861 ctcaatgaca agatccctgt cctcccgggc ttgacccggg atccatgaat ttcggatcga
     4921 gcccgcaccg gcggacatcg ctacttcttg gccccgaagt ccacctgcgg cggcaccgaa
     4981 atctgcgcct cgttctgcac ggtgaacgtc ggcttgggat cgtaattgaa caccatcgcc
     5041 gtgaggttgg tggggaagct gcgcgcgagc acgttgtact gctgcaccgc ctgaatatag
     5101 cggttgcgcg ccaccgtgat gcggttctcg gtgccttcca gctgcacgcg cagctcacgg
     5161 aaactctgat tggccttcag gtccggatag cgttccacca cgaccatcag gcggctcagc
     5221 gcgctggaca gctcaccctg cgcctgctgg aacttggcga acgcctcggg gttgttgatg
     5281 gtttccggcg tgacctgcat ggaagtggcc ttggcgcgcg cctcgaccac cttgctcagc
     5341 gtgtcctgct cgaagctggc ctcgcccttg accgtgttga ccaggttggg caccaggtcg
     5401 gcgcgccgct ggtactggtt gagtacttcg ctccaggccg acttggtctg ctcgtccagc
     5461 cgctggaaat cgttgtagcc gcagcccgtg agcgtcaggg ccgccaggat catgaggagc
     5521 aagcgtttca ctatcttctt cccttcggtg agagaggcaa cggtagcata gatgggatgc
     5581 gttccggccc gattcaagtg ctgcaggcgg cccagcgacc gggctccccc gagcgcaagc
     5641 cgcgcctgct catcgccggc gcgaccggcg cgctgggcaa cgaagtgctg cgccgcctgg
     5701 tcggcttggg ccggttcgaa agcacccagg tgctggctcg agagcccatc actcccgcgc
     5761 tgcgcggcgt cacggccacc gtggtgagtt ccggctcgcc ctcgcagtgg atgcccactt
     5821 cggccgaggc cggcgtcatc ctgttcgacc cgccgcgcct gttccatgac cgggagcgcg
     5881 cactctggac accgggtccg gaccagttgc cggacgtggc gcagtggatg cgccgcagcg
     5941 gcgtgcagac gctggcggtg gtgctgccgc atgcgcaggg aacgctgccg gaagctctca
     6001 agcgtggcct ggccagcctc gacgagcagg ctgtcgcggc gctcggtttc gagcgcctgc
     6061 tgctcatccg ctcggcgcgc aagccaggcg cagtgaatac gcgtgagctt cttcccaggc
     6121 tcgcgcactg gatgctctcg gttgccaggt tcatggtgcc cagcagtgaa cagccggtgc
     6181 gggcgtcgaa agtggccgaa ctggtggcag tggccctgca gctggcgccg cccggcatcc
     6241 acatcgcggc gcccgaactg gtgtggcgcg ccgcccaagg tgacctgcgc gagactgcgc
     6301 ggcagtggct gcacttctga atcaaggcaa aatcgcagca cgaaccgttt catgcaaaaa
     6361 agcaaactcc aggccgccaa tctcgaaggc aacgcagacg atgtcgaagc cgcgttctac
     6421 gacgcgctgc aaaatggcga catcgaccgg ttgatggcct gctgggccga cgaggacgac
     6481 atcgtctgca tccatcccgg cggcccgcgc ctggtggggc ccgtggcgat ccgtgcggcc
     6541 ttcgaatcca tgttcaccaa cggcagcata cgcgcctggc ccgagcgcgc acgcaagatt
     6601 caggcggtgg ccagcgcagt gcacaacgtg ctcgagcgca tcgaggtgct gacgccagag
     6661 ggtccgggcc aggcctgggt catcgccacc aacgtctacc acaggacggc gcaaggatgg
     6721 cgcatggtcg cgcaccacgc cagccccgga accgacagcg acatccagga ggtctccggc
     6781 gatccgcagg tgctgcattg aaatgaaagc tcccccccga agcgccttcg gcg
//
LOCUS       DASWIY010000066        14523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1897569, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000066 DASWIY010000000
VERSION     DASWIY010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 14523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14523
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897569"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VGA59_02500"
     CDS             complement(<1..478)
                     /locus_tag="VGA59_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9718574.1"
                     /translation="MACVASMALAQGKSYPGIGRDATPKEIGAWDIDVRPDFKGLPAG
                     SGSVAKGQDVWEAKCASCHGVFGESNQVFNPLVGGTSADDIKTGRVASLKRPDYPGRT
                     TMMKLSSISTLWDYINRAMPWAQPKSLSVEEVYAVTAYLLNLADVVPADFTLSDKNI"
     gene            complement(582..1925)
                     /gene="soxC"
                     /locus_tag="VGA59_02505"
     CDS             complement(582..1925)
                     /gene="soxC"
                     /locus_tag="VGA59_02505"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEX9718575.1"
                     /translation="MNAGRLRKAPENFLNRQGITEVAAQARSGRRDFLRGAFAAAAAG
                     MALPAARAQNEAPADGDPNILNLPDHSRGLGQAVATDGYGKPSKFESNVHRRRSPGLT
                     QTTQASVSFAPLQSLFGIVTPSGLHFERHHQGWWDIDPSKHRFMVNGMVKTAKVFTMD
                     ELMRLPSVSRFHFIECGANTGMEWGNVAVPTAQYTHGMLSCSEFTGVPLITILEQCGA
                     DLAKGKFVLAEGGDGSSMTRTIPMSLIRSGEVLVAYGQNGEMLRPENGYPLRLVVPGV
                     QGVSWVKYLRRVEVGDQPYGAKDETIHYVDLMPDGQHRQYTSIQECKSVVTTPSGGQV
                     LLDKGFYNVTGLAWSGRGKVKRVDVSVDGGRNWRTARLEGPVMSKCLTRFSIDWVWDG
                     KPAIIQSRATDETGYVQPTYRQLRAVRGTRSIYHNNAIQSWLVEQGGEVKNVQVG"
     gene            complement(1948..2250)
                     /locus_tag="VGA59_02510"
     CDS             complement(1948..2250)
                     /locus_tag="VGA59_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9718576.1"
                     /translation="MKVLILAAAVLAAAPVFAGKELATKNACLACHATDKKLVGPAYQ
                     DVAKKYAGQKDAEATLAKSIKAGGSGKWGPVPMPAQAALSDADAKTLAAWVLGGAK"
     gene            complement(2410..3447)
                     /gene="fba"
                     /locus_tag="VGA59_02515"
     CDS             complement(2410..3447)
                     /gene="fba"
                     /locus_tag="VGA59_02515"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953000.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX9718577.1"
                     /translation="MALISLRQMLDHAAEHGYGVPAFNVNNLEQIQAIMEAAQETGSP
                     VILQASAGARKYAGEAYLRHMVLAAIESHPDLPVVLHQDHGATPAVCQQSIRSGFSSV
                     MMDGSLREDAKTPANYEYNVEVTRKVCEMAHAVGVSVEGELGCLGSLETGEAGEEDGV
                     GAVGKLPADMLLTDPVQARDFVAKTGVDALAIAIGTSHGAYKFSRKPTGDILAIDRIA
                     SIHAQIPDTHLVMHGSSSVPLEWLQIIRHYGGEIKETYGVPVEEIRRGIAGGVRKINI
                     DTDIRLAMTGAMRKAFALDRAEFDPRKALIAARNAARGICKARFEAFGCAGQATRIKP
                     VALEQMIRRYA"
     gene            complement(3608..3730)
                     /locus_tag="VGA59_02520"
                     /pseudo
     CDS             complement(3608..3730)
                     /locus_tag="VGA59_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3744..4628)
                     /locus_tag="VGA59_02525"
     CDS             complement(3744..4628)
                     /locus_tag="VGA59_02525"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEX9718578.1"
                     /translation="MSERHPIIAITGSSGAGTTSVTRTFENIFRREKVNAAIVEGDSF
                     HRYDREEMKARQAEAEHAGDRHFSHFGPENNLFAELAQLFRSYGETGQGLRRKYLHDA
                     DEAAPHRQEPGTFTPWENLPEGTELLFYEGLHGAVVTPEVNIAKYPDLLIGVVPVINL
                     EWIQKLWRDKSKRGYSTEAVTDTILRRMPDYVNYICPQFTHTHVNFQRVPCVDTSNPF
                     IAREIPAPDESFVVIRFAQPKGIDFQYLLSMIHDSFMSRANTIVVPGGKMELAMQLIF
                     TPFIWRMVERRKRALGTV"
     gene            complement(4663..5751)
                     /locus_tag="VGA59_02530"
     CDS             complement(4663..5751)
                     /locus_tag="VGA59_02530"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX9718579.1"
                     /translation="MPLSNRLTLTQYLIDHRRRFPAATGDFNALILDVALACKAIAKA
                     VACGELGGTLGRQPGENGICVNVQGEQQMKLDVLANEIFLRMNEWGGHLAGMASEELE
                     QPYAIPGRYPRGKYLLLFDPLDGSSNIDVNLSVGSIFSVLRAPPEAMRGELEEAHFLQ
                     PGRQQVAAGYALYGPVTMLILTVGDGVAGFTLDPVLGEFMLTHPQLQVPAETQEFAIN
                     ASNRRFWEPPVQRYVEECQAGRDGPRGKDFNMRWIASLVAEAHRILMRGGVFLYPRDN
                     KDPARAGRLRLLYEANPVGFIIEQAGGRASTARQSVLGVQPDSLHQRVGLVFGSRSEV
                     ERIERYHAEPGVHDIAAPLFAERSLFTA"
     gene            complement(5782..6732)
                     /gene="cbbX"
                     /locus_tag="VGA59_02535"
     CDS             complement(5782..6732)
                     /gene="cbbX"
                     /locus_tag="VGA59_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HEX9718580.1"
                     /translation="MNQPLHLIPGMRAQAPAAVTFPAEATAAKSVADVLAESQVEAVL
                     HELDRDLVGLAPVKARIRDIAALLVIDKLRLNLGLQAQAPSLHMSFTGNPGTGKTTVA
                     MRMAQILHRLGYVRKGHLVAVTRDDLVGQYIGHTAPKTKEVLKRAMGGVLFIDEAYYL
                     YRPENERDYGQEAIEILLQAMENQRDDLVVILAGYQDRMETFFRSNPGMSSRVAHHIA
                     FPDYQPDELMAIADKMLHAQNYALGEGARDALDRYVGLRIGQPHFANARSIRNALDRA
                     RLRQASRLFGDRDRELTARDLTTIEAGDILASRVFANAAA"
     gene            complement(6768..7202)
                     /locus_tag="VGA59_02540"
     CDS             complement(6768..7202)
                     /locus_tag="VGA59_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HEX9718581.1"
                     /translation="MMTNPTGRLTQGQFSFLPDLTDAEIAMQIQWGLDKGYAWSVEYT
                     DDPHPRNTYWDMFGMPMFDLRDAAGVLAEVKECRKAFPHHYIRLMAFDSTRGVESIAM
                     SFLVNRPASEPGFFLERQEGQGRSTRYATRGYALGKPEMERY"
     gene            complement(7223..8677)
                     /locus_tag="VGA59_02545"
     CDS             complement(7223..8677)
                     /locus_tag="VGA59_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HEX9718582.1"
                     /translation="MGERQITDAKTRYSAGVLKYRQMGYWMPDYVPKDTDTICLFRIT
                     PQEGVDPEEAAAAVAGESSTATWTVVWTDRLTACDSYRAKAYRVEAVPNNPGQYFAYV
                     AYDLILFEEGSIANMTASLIGNVFSFKPLKAARLEDIRIPVAYVKTFKGPPTGLVAER
                     ERLDKFGRPLLGATTKPKLGLSARNYGRVIYEGLKGGLDFMKDDENINSQPFMHWRDR
                     FLYVMEAVNKAQAATGEVKGSFLNVTAGTMEAMYERAEFAKNLGSIVVMVDLVVGWTA
                     IQSMAEWCRRNDMLMHMHRAGHGTYTRQKNHGVSFRVIAKWLRLAGCDHLHTGTAVGK
                     LEGDPLTVQGYYNVCRDSVTKTDLPRGLYFDMDWADTRKVMPVASGGIHAGQMHQLIE
                     LFGDDVILQFGGGTIGHPQGIQAGAVANRVALETMVKARNEGRDILQEGPEILRRAAQ
                     SCGPLRAALDTWGDITFNYESTDSSDYVATPALA"
     gene            8769..9716
                     /locus_tag="VGA59_02550"
     CDS             8769..9716
                     /locus_tag="VGA59_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9718583.1"
                     /translation="MKNATFRQLRVFSEVARHLSFAKAAQVLHLSPPAVTMQIKELEG
                     HVGLPLFDRSGRSVTLTTPGEYMLVYARRMLSTLKDAEDAAARLKQVEAGTLTIGMVG
                     TAKYFLPRLLAQFRQEHEGVEIRLVEGNRSQLVRMLQANDVDIAVMGRPPKELATRAE
                     PFAAHPHVFVAPVGHPLLGEEGGTTGVESLRPWDFIVREEGSGTRAALEKFLQEAHLE
                     PRVIMEMGSNETIKQAVMAGMGISFLSLHTLQLELDNGLLALLDVAGTPVVRAWNVVH
                     TLSKVLSPAAEAFRYFILERGEAYLAQHFAGHLRLLHAG"
     gene            complement(9746..10174)
                     /locus_tag="VGA59_02555"
     CDS             complement(9746..10174)
                     /locus_tag="VGA59_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX9718584.1"
                     /translation="MNRAVRFLAIVTGSLLVWGAALAQEKVVYHVDNSELQATKGLRN
                     VRNHLDVAPDTRIVVVTHADGVDFLMEGARDKKNPNIDYGSLVSALKARGVRFEVCEI
                     TLRNRNLKKEQFILDAEFTPSGVVRIAQLQSRDKFAYIKP"
     gene            complement(10315..11271)
                     /locus_tag="VGA59_02560"
     CDS             complement(10315..11271)
                     /locus_tag="VGA59_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9718585.1"
                     /translation="MRHSGVLIRVLAWLAVACHAMPAWAQPAAAAVVLRQVSASAWYV
                     QGESALGSPANRNFISNAGIVVTPAGVVVIDALGSPQLARELMAEVRRSTGKAVTHVI
                     LTHYHADHIYGLQEFKRAGARIVAHEAALEYLNSETAQARLEVSRKDLAPWIDAQTQL
                     VPADEWIAGPRELVVGGVRLLLQPVGPAHTPEDTVVFLPQEKVLFAGDLVFRGRIPFV
                     GQADSGHWIKALNTVLGFDAAVVVPGHGPMSTSAREDMELTRDYLGYLRETMGRAARD
                     MDPFEDAYRRTDWSRFEHLPLFRVANRMNAYNTYLLMEREGN"
     gene            complement(11429..11773)
                     /locus_tag="VGA59_02565"
     CDS             complement(11429..11773)
                     /locus_tag="VGA59_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9718586.1"
                     /translation="MPKRSNNSAPTDSAVESDEVFELAAEIFRVMSAPMRLKIIASLC
                     NGEKNVSELLAEIDTTQPNMSQHLNTLYQAGVLGKRRDGVQIYYRIVNDRVVTLCRAV
                     CTQIAIESDVAR"
     gene            complement(11885..13024)
                     /locus_tag="VGA59_02570"
     CDS             complement(11885..13024)
                     /locus_tag="VGA59_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX9718587.1"
                     /translation="MTSVEVALLTQQVLWASFFVSMAFGAVVQRTGFCTMGAVSDVVT
                     MGDWTRMRQWGLAAGVAVVGFGLLAWAGLVSPDKTLYASNRWTWLSALAGGGIFGFGM
                     VLASGCASKALVRVGGGNLKAVVVVVVLGVAAFATLKGLTAVWRTETVDRVALDFQAQ
                     ASLPAWAAQAFAAKASAAGLALSLVIGGALVAWALAGREFLRGTNVLAGAGVGAGVVA
                     MWWISGHVGHIAEHPETLQETFLATNSTRAEALSFVSPIAYTLDWLMFYSDRNKSLSV
                     GIVSVFGVIAGSLSVSLAQRSFRWEGFGGTDDLAHHLGGAVLMGVGGVTAMGCSIGQG
                     VSGISTLSLTSFVAVGAMIAGAAAALRYQVWRLEREAGATAARTP"
     gene            13463..13849
                     /locus_tag="VGA59_02575"
     CDS             13463..13849
                     /locus_tag="VGA59_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9718588.1"
                     /translation="MPQSNSPVRVFLADDSALIRERVAAMLAARAMAIVGHAETPQGS
                     IDGILAARPDVVVLDVQLEGGSGLQVLRAIRQSDPDIAFVVFSNNSGPAYRKRYLGEG
                     ALRFLDKSTEFDQLVPAVETASQHAN"
     gene            13874..>14523
                     /locus_tag="VGA59_02580"
     CDS             13874..>14523
                     /locus_tag="VGA59_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9718589.1"
                     /translation="MSAATLEPNVSRIRIMSAAPSVQAQPATAATTQCSTCHLRDLCL
                     PSGMAAPDVDRLDNMKFARRKVKAGQTLYREGDRFQYIYAVRTGTLKSSLMLADGREQ
                     VSGFHIAGELVGLDGAAQGHHASATMALEDTDICAIPYAHLTDLAASVSGMQHVISRL
                     MSREILREHSLMMLLGSMNADERLAAFLLNLSQRYAARGYSEREFHLRMSRAEIGS"
BASE COUNT         2351 a         4760 c         4809 g         2603 t
ORIGIN      
        1 ggatgttctt gtccgacagc gtgaagtcgg caggcaccac gtcggccagg ttgagcagat
       61 aggccgtgac cgcgtagacc tcctcgacgc tgagcgactt tggttgcgcc cacggcatcg
      121 cgcggttgat gtaatcccac aacgtggaga tgctggacag cttcatcatc gtggtgcggc
      181 ccgggtagtc gggccgcttc aggctggcca cgcggccggt cttgatgtcg tcggcgctgg
      241 tgccaccgac gagcggattg aacacctggt tcgactcgcc gaagacgccg tggcatgagg
      301 cgcacttggc ttcccacacg tcctgtccct tcgcgacgct gccggagccg gcgggcaggc
      361 ccttgaaatc ggggcgcacg tcgatatccc acgcaccgat ttctttcgga gtcgcatcgc
      421 ggccaatgcc cgggtacgac ttgccttgcg ccaaggccat gctcgcgaca caagccataa
      481 cggcggcgat cgctttcggg gccaccgcgg aaccggcttt gccgggccgc tggtggcgcc
      541 cccttgaggg ggaggcggcc gcaggccgct tcgggggggg gctatcccac ctggacattc
      601 ttcacctcgc cgccttgttc gaccagccag gactggatgg cgttgttgtg atagatcgat
      661 cgcgtaccgc gcactgcgcg cagttgccgg taggtcggct gcacatagcc ggtttcgtcg
      721 gtggcgcggc tctggatgat ggccggcttg ccgtcccaca cccagtcgat cgagaaacgc
      781 gtgaggcact tgctcatcac agggccttcc agccgggcgg tgcgccagtt gcggccgccg
      841 tccacggaca cgtcgacccg cttgaccttg ccgcgtcccg accatgcgag cccggtcacg
      901 ttgtagaagc ccttgtccag cagcacctgg ccgcccgacg gcgtggtgac cacgctcttg
      961 cattcctgga tgctggtgta ctggcggtgc tgcccgtcgg gcatcaggtc cacgtaatgg
     1021 atcgtctcgt ccttcgcccc gtagggctgg tcgcccactt ccacgcggcg caggtacttg
     1081 acccagctca cgccctgcac gccgggcacc acgagccgca ggggatagcc gttttcgggc
     1141 cgcagcatct cgccgttctg gccgtaggcc accagcactt cgcccgagcg gatcaggctc
     1201 atgggaatgg tgcgcgtcat ggacgagccg tcgccgcctt cggcgagcac gaacttgcct
     1261 ttggccaggt ccgcgccgca ctgctccagg atggtgatca gcggcacgcc ggtgaactcg
     1321 ctgcacgaca gcatgccgtg cgtgtactgg gccgtgggca cggccacgtt gccccattcc
     1381 atgccggtgt tcgcgccgca ttcgataaag tggaagcgcg acaccgaggg caatcgcatc
     1441 agctcgtcca tggtgaacac cttggccgtc ttcaccatgc cgttgaccat gaagcgatgc
     1501 ttggacggat cgatgtccca ccagccctgg tggtggcgct cgaaatgcag gccgctgggc
     1561 gtcacgatgc cgaacagcga ttgcagcggc gcgaaggaga ccgaagcctg cgtcgtctgc
     1621 gtcaggccag ggctgcggcg gcggtgcaca ttcgattcga atttggatgg tttgccgtag
     1681 ccgtctgtgg ccactgcctg gcccagtccc ctggaatggt ccggcaggtt gaggatgttc
     1741 gggtcgccgt cggccggcgc ctcgttctgc gcgcgcgcgg cgggcagcgc catccccgcg
     1801 gcggctgcgg cgaaggcgcc gcgcagaaaa tcgcgccggc cgctgcgtgc ctgcgcggct
     1861 acctcggtaa tgccttggcg gttcaggaag ttttcaggcg ctttgcgcag ccggccggcg
     1921 ttcatggcgt tccttctgtc tctgcgatca cttcgctccg cccagcaccc aggccgcgag
     1981 cgtcttggcg tctgcatcgc tgagtgccgc ctgggccggc atcggcaccg ggccccactt
     2041 gccggatccg cccgccttga tgctcttggc gagcgtggcc tcggcgtcct tctggcccgc
     2101 gtacttcttg gccacgtcct ggtaggcggg gcccaccagt ttcttgtcgg tagcatggca
     2161 cgccagacag gcgttcttcg tggccagttc cttgccggca aataccgggg cggcggccag
     2221 cacggcggcg gcgaggatca gaaccttcat ttcggaaatt ttcctaaatc agaacacgcg
     2281 aatataaaga gcggcgaata tatcatgaac gcctgcggcc tgcagggctc gcctgcgctt
     2341 tttcgtcggg ggaaacccgg atcgcgcgcc gggtgaaaaa cccgggaaga cgtgccgggc
     2401 aagaaccgat caggcgtagc gccggatcat ctgctcgagc gcaaccggct tgatacgcgt
     2461 ggcctgcccg gcgcaaccga aagcttcgaa gcgcgccttg cagatgccgc gggctgcgtt
     2521 gcgggccgcg atcagtgcct tgcgcggatc gaactccgcg cgatccagcg cgaaggcctt
     2581 gcgcatggcc ccggtcatcg ccagccggat gtccgtgtcg atgttgatct tgcgcacgcc
     2641 gccggcgatg ccgcgccgga tttcctccac cggcacgccg taggtctcct tgatttctcc
     2701 accgtagtgc cgaatgatct gcagccactc cagcggtacg ctggaactgc cgtgcatcac
     2761 cagatgcgta tcgggaatct gcgcgtggat ggaggcgatg cggtcgatgg cgaggatgtc
     2821 gcccgtcggc ttgcgcgaaa acttgtaggc gccatgactg gtgccgatgg cgattgccag
     2881 cgcgtccacg ccggtcttcg ccacaaagtc gcgcgcctgc accggatcgg tcagaagcat
     2941 gtccgcgggc agcttgccca ccgcgccgac cccgtcctcc tcgccggcct ctccggtttc
     3001 cagcgagccg aggcaaccca gttcgccttc gacggatacg ccgacggcgt gtgccatttc
     3061 gcagactttg cgcgtcacct cgacgttgta ctcataattg gccggcgttt tggcgtcctc
     3121 gcgcagcgag ccgtccatca tcacgctgga gaagcccgag cggatcgact gctggcacac
     3181 ggccggggtg gcgccatggt cctggtgcaa cacgaccggg aggtcaggat gcgattcgat
     3241 ggcggcgagc accatgtgcc gcaggtaggc ctcgcccgcg tacttgcgcg cgccggcgga
     3301 agcctgcagg atcacagggc tgcccgtttc ctgcgcggcc tccatgatgg cctggatctg
     3361 ttccaggttg ttgacgttga aagccggcac gccgtagccg tgctcagcgg cgtggtcgag
     3421 catctggcgc agtgatatca aggccatggg aaatgttctc ctgtgattga aattcgaccg
     3481 ttcccgccaa gcagcgcgcg caggtcgccg atcagccgat cgggcgcctg gcgcgcgatc
     3541 ggctggccct ggttgtagcc ataggtcacg ccccacacgg ctatgccggc gttgcgcgcg
     3601 gcggataggc gatgtcggcc atgcccatgg gggcgccggg atggcctgaa ctggcgcgct
     3661 cgacggcatc gacggcgagc atgcgcagcg cgtccgccat gcggcgctgg aggtcgaagg
     3721 tgtggttcat cctgtgttcc ctttcagacg gtgccgagcg cgcgcttgcg gcgctccacc
     3781 attcgccaga tgaacggcgt gaagatcagc tgcatggcca gttccatctt tccgccgggc
     3841 accacgatcg tgttggcgcg cgacatgaag gagtcgtgga tcatgctgag caggtactgg
     3901 aagtcgatgc ccttgggctg ggcgaagcgg atcaccacga agctctcgtc gggagcggga
     3961 atctcgcgcg ctatgaaggg attggacgta tcgacgcaag gcacccgctg gaaattcacg
     4021 tgcgtatggg tgaactgcgg gcagatgtag ttcacgtagt cgggcatgcg gcgcaggatg
     4081 gtgtcggtca cggcttcggt cgaatacccg cgcttggact tgtcgcgcca caatttctgg
     4141 atccattcca ggttgatcac cgggaccacg ccaatcagca ggtcgggata cttcgcgatg
     4201 ttcacctccg gcgtgaccac cgctccgtgc aggccttcgt agaacaggag ctcggtgccc
     4261 tcgggcaggt tctcccatgg cgtgaaggtg ccgggctcct ggcggtgcgg cgcggcttcg
     4321 tcggcgtcgt gcaggtactt gcggcgcaga ccctgcccgg tttcgccgta gctgcgaaac
     4381 agctgcgcga gctccgcgaa gaggttgttt tccggcccga aatggctgaa gtggcggtct
     4441 cccgcgtgct cggcctcggc ctggcgggcc ttcatctctt cgcggtcgta gcggtggaaa
     4501 ctgtcgccct cgacgatggc ggcgttcacc ttctcccgcc ggaagatgtt ctcgaaggtg
     4561 cgtgtcaccg aagtggtgcc ggcgccggac gagccggtga tggcgatgat gggatggcgt
     4621 tccgacatgg gtggctcctt atggtcaagt ccgtgccccg gctcaggccg tgaacaggct
     4681 gcgttcggcg aacaggggcg cggcgatgtc gtgcacgccc ggctcggcgt gatagcgctc
     4741 gattcgctcc acctcgctgc gcgatccgaa cacgaggccc acgcgctggt gcagcgaatc
     4801 cggctgcacg cccagcacgc tctggcgggc ggtgctggcg cgaccgccgg cctgctcgat
     4861 gatgaaaccg accgggttgg cttcgtacag cagccgcagc cggccggcgc gagccgggtc
     4921 cttgttgtcg cgcgggtaca ggaacacgcc accgcgcatg aggatgcggt gcgcctccgc
     4981 gaccagcgag gcgatccacc gcatgttgaa gtccttgccg cgtggaccgt cgcggccggc
     5041 ctggcactct tccacgtagc gctgcaccgg cggctcccag aagcggcggt tggaggcgtt
     5101 gatggcgaac tcctgcgtct cggccggcac ctgcaactga gggtgggtca gcatgaactc
     5161 gcccagcacg gggtcgaggg tgaatcccgc gacgccgtcg cctaccgtga ggatcagcat
     5221 ggtgacggga ccgtaaagcg cgtagccggc cgcgacctgt tgccggcccg gctgcaggaa
     5281 atgggcttcc tccagttcgc cgcgcatcgc ttcgggcggc gcgcgcagca cggagaagat
     5341 gctgcccacc gagaggttca cgtcgatgtt gctggaaccg tccagcggat cgaacagcag
     5401 caggtatttc ccgcgcggat accggccagg gatggcgtag ggctgctcga gttcctcgct
     5461 ggccatgccg gcgaggtggc cgccccattc gttcatgcgc aggaagatct cgttggcgag
     5521 cacgtcgagc ttcatctgct gctcgccctg cacgttgacg caaatgccgt tctcgcccgg
     5581 ctgccgaccg agcgtgccgc ccagctcgcc gcaggcgacc gccttcgcga tggctttgca
     5641 ggcgagggcc acgtcgagga tcagcgcgtt gaagtctccg gtcgctgccg gaaagcggcg
     5701 ccggtggtcg atcaggtatt gggtgagggt gagccggttg gacaagggca tggcgatcct
     5761 tcgtgaagtt tgaaacgttg ctcaggccgc ggcattcgcg aacacccggc tggcgaggat
     5821 gtcgccggcc tcgatggtgg tgaggtcgcg cgccgtgagt tcgcgatcgc ggtccccgaa
     5881 caggcgactg gcctggcgca ggcgcgcgcg gtccagcgcg tttctgatgc tgcgggcgtt
     5941 ggcgaagtgg ggctgcccta tgcgcagtcc gacgtaacgg tcgagcgcgt cgcgcgcgcc
     6001 ttcgccaagc gcgtagttct gcgcgtgcag catcttgtcc gcgatggcca tcagctcatc
     6061 cggctggtaa tccgggaacg cgatgtgatg cgccacccgg gaactcatgc cggggttgct
     6121 gcggaagaac gtctccatcc ggtcctggta gccggccagg atcaccacca ggtcgtcgcg
     6181 ctggttttcc atggcctgca gcaggatctc gatggcctcc tgcccgtagt cgcgctcgtt
     6241 ctccggccgg tagaggtagt acgcctcgtc gatgaacagc acgccgccca tcgcccgctt
     6301 gagcacctct ttcgtcttgg gcgccgtgtg gccgatgtac tggcccacca ggtcgtcgcg
     6361 ggtgaccgcc accaggtgcc ctttgcgcac gtagcccagg cggtgcagga tctgggccat
     6421 gcgcatcgcc accgtcgtct tgccggtgcc tgggttgccg gtgaagctca tgtgcaggct
     6481 gggcgcctgg gcctgcaggc ccagattcag ccgcagcttg tcgatcacca gcagcgcggc
     6541 gatatcgcgg atgcgcgcct tgaccggcgc cagtccgacg aggtcgcggt cgagttcgtg
     6601 caacactgcc tcgacctgcg attcggccag cacgtccgcg actgatttcg ccgccgtcgc
     6661 ctctgccggg aatgtgacgg cagcgggggc ctgcgcccgc atgccgggga tcaggtgaag
     6721 aggttggttc attgcgttgg cgctcgtggg ctagccgcgg ctcgtcctca gtaccgctcc
     6781 atctcgggct tgcccaaggc atagccgcgt gtcgcatagc gcgtcgagcg tccctggccc
     6841 tcttgccgct cgagaaagaa gcccggctcc gacgcgggcc ggttcaccag gaagctcatg
     6901 gcaatcgact ccacgccgcg cgtcgaatcg aacgccatca ggcggatgta gtggtggggg
     6961 aacgccttgc ggcattcctt cacttccgcc agcacgcctg cagcgtctcg caggtcgaac
     7021 atcggcatgc cgaacatgtc ccagtaggtg ttgcgcggat gcgggtcgtc cgtgtactcc
     7081 acgctccagg cgtagccctt gtccaggccc cactggatct gcattgcgat ttcggcatcg
     7141 gtgaggtcgg gcaggaaact gaattggccc tgggtcaggc ggccggtcgg gttggtcatc
     7201 atgttgcgtg ctctctttcg gttcaggcga gcgcgggcgt ggccacgtaa tcgctgctgt
     7261 cggtggactc gtaattgaaa gtgatgtcgc cccaggtatc gagcgcggcg cgcagcggtc
     7321 cgcagctttg cgcggctcgc cgcaggatct ccgggccttc ctgcaggatg tccctgcctt
     7381 cattgcgcgc cttcaccatc gtttccaggg ccacgcgatt ggcgaccgcg ccggcctgga
     7441 tgccttgcgg gtggccgatc gtgccgccgc cgaactgcag gatcacgtcg tcgccgaaca
     7501 gctcgatgag ctggtgcatc tgcccggcgt ggatgccgcc ggaggccacc ggcatcacct
     7561 tgcgggtgtc ggcccagtcc atgtcgaaat agaggccgcg cggcaggtct gtcttcgtga
     7621 cgctgtcgcg gcagacgttg taatagccct gcacggtgag cgggtcgcct tcgagcttgc
     7681 ccacggcagt gccggtatgc aggtggtcgc atcccgccag ccgcagccat ttggcgatca
     7741 cccggaagct cacgccgtgg ttcttctgcc gggtgtacgt gccgtgaccc gcccgatgca
     7801 tgtgcatcag catgtcgttc ctgcggcacc actcggccat cgactggatg gcggtccagc
     7861 cgacgacgag gtccaccatc accacgatcg agcccaggtt cttggcgaac tccgcccgct
     7921 cgtacatggc ttccatcgtt ccggcggtga cgttcaggaa ggaacccttg acttcgccgg
     7981 tggcggcctg cgccttgttc accgcttcca tcacgtacag gaaacggtcg cgccaatgca
     8041 tgaacggctg cgagttgatg ttctcgtcgt ccttcatgaa atccagcccg cccttcaggc
     8101 cttcgtagat cacgcggcca tagttgcgcg cggacaggcc gagcttgggc ttggtcgtgg
     8161 ccccgagcag ggggcggccg aacttgtcca ggcgttcgcg ctcggccacc aggcccgtgg
     8221 ggggcccctt gaaggtcttg acgtaggcca ccgggatgcg gatgtcttcc aggcgcgccg
     8281 ctttcagcgg cttgaaggaa aagacgttgc cgatcaggct ggccgtcatg ttggcgatgg
     8341 agccttcctc gaacaggatc aggtcatagg cgacgtaggc gaaatactgg cccgggttgt
     8401 tgggaacggc ctcgacccgg tacgccttcg cgcggtagct gtcgcaggcc gtgagccggt
     8461 cggtccacac gacggtccag gtggccgtgg acgactcgcc cgcgacggct gccgccgcct
     8521 cctcgggatc gaccccttcc tggggcgtga tgcggaacag gcaaatggtg tcggtgtcct
     8581 tggggacgta atccggcatc cagtaaccca tctgccggta cttgaggacg cccgcgctgt
     8641 atcgcgtctt ggcatcggtg atctggcgct cgcccatcga tagctcctgg tgtgttcgag
     8701 tgccggacta taagcagcaa cttaaataag gtaaattcag actttattta ctctcagtta
     8761 agcaattcat gaagaacgcc acgtttcgcc agctgcgcgt gttcagtgaa gtggcgcgcc
     8821 acctgagctt tgccaaagcc gcgcaagtac tgcacctgtc accgcccgcc gtcaccatgc
     8881 agatcaagga actggaaggg cacgtgggcc tgcctttgtt cgaccgcagc ggccgcagcg
     8941 tgaccctgac caccccgggc gagtacatgc tggtctacgc gcgccggatg ctttccacgc
     9001 tcaaggatgc cgaggacgcg gcggcgcgat tgaagcaagt cgaggcaggg accctgacca
     9061 tcggcatggt cggcacggcg aaatacttcc tgccgcgcct gctggcccag ttccgccagg
     9121 agcacgaggg ggtggagatc cggctcgtcg aaggcaaccg cagccagctg gtgcggatgc
     9181 tgcaggccaa cgatgtcgac atcgccgtga tgggccggcc gcccaaggaa ctggcgaccc
     9241 gcgccgaacc gttcgcggcg catccgcacg tgttcgtcgc gcctgtcggg catccgttgc
     9301 tcggcgagga gggcggcacg accggcgtcg agtcattgcg cccctgggac ttcatcgtcc
     9361 gcgaagaggg ttccggcacc cgcgccgcgc tcgaaaagtt tctgcaggag gcgcacctcg
     9421 agccgcgcgt catcatggag atgggaagca acgagaccat caagcaggcg gtcatggccg
     9481 gcatggggat cagcttcctc tcgctgcaca ccctgcaact ggagctggac aacggcctgc
     9541 ttgcactgct cgacgtcgcc ggcacgccgg tcgtacgggc atggaacgtc gtccacacgc
     9601 tttccaaggt gctgtcaccc gcggccgagg cgtttcgcta cttcatcctg gaacgcgggg
     9661 aagcctacct cgcgcagcac ttcgccggcc acctgcgcct gctgcatgca ggctgagcgg
     9721 tgcagccgat cgaacgcgcg acggatcagg gtttgatgta cgcgaacttg tcccgcgact
     9781 gcagctgcgc gatccgaacg actccggacg gcgtgaactc ggcatcgaga atgaactgct
     9841 ccttcttgag gttgcggttg cgcaacgtga tctcgcacac ctcgaaccgg acgccgcggg
     9901 ccttcagcgc actgaccagc gaaccgtagt cgatgttcgg gttctttttg tcgcgggcgc
     9961 cctccatcag gaaatccacg ccgtcggcat gtgtgacgac aacgatcctg gtatcggggg
    10021 ccacgtccag atgattgcgg acgttgcgca gccccttggt ggcctgcagt tcggagttgt
    10081 cgacgtgata gaccaccttt tcctgggcca gggccgcgcc ccagacgagc aggctgccgg
    10141 tgacgatggc aagaaatctg acggcgcggt tcatcgcgag tctccttgtg tgggtgaaat
    10201 ccaggctgac gggcatgatt cgccctggac accattaaat catttgatgc agatcaaacg
    10261 atatagggtt ttcgctatcg agggcgagcc cggccatcgc gccagcgcgc agggtcagtt
    10321 gccttcacgc tccatgagca ggtaggtgtt gtacgcgttc atccggttgg caacgcggaa
    10381 cagcggcaga tgctcgaaac gcgaccaatc cgtgcgccgg taggcgtcct cgaacggatc
    10441 catgtcgcgc gcggcccggc ccatcgtttc gcgcaggtaa cccaggtagt cgcgggtcag
    10501 ctccatgtct tcgcgcgccg aagtggacat cgggccatgg ccggggacca ccacggcggc
    10561 atcgaagccg agcaccgtgt tcaacgcctt gatccagtgc ccgctgtcgg cctggccgac
    10621 gaagggaatg cgtccgcgaa acacgaggtc gcccgcgaac agcaccttct cctgcggcag
    10681 gaacaccacc gtgtcttcgg gcgtgtgcgc cgggccgacc ggctgcagca gcaggcgcac
    10741 gccgccgacg accagttcgc gcggcccggc tatccattcg tcggccggca ccagctgcgt
    10801 ctgcgcgtcg atccatggtg cgaggtcctt gcgggacact tcgaggcgtg cctgtgcggt
    10861 ctcggagttc aggtactcga gcgcggcctc gtgggcgacg atgcgcgcgc ctgcgcgctt
    10921 gaactcctgc agcccataga tgtggtcggc gtggtaatgg gtgaggatca catgcgtcac
    10981 ggcctttccg gtgctgcgcc gcacctcggc catcagctcg cgcgcgagct gcggcgagcc
    11041 gagcgcgtcg atgacgacca cccccgcggg cgtgacgacg ataccggcat tggagatgaa
    11101 attgcgattg gccgggctgc ccagcgccga ctcgccctgc acgtaccacg ccgacgccga
    11161 cacctgccgc aggacgaccg cggcggccgc cggctgcgcc caggcaggca tggcatggca
    11221 ggcgaccgcg agccacgcga gtacgcgaat caggactccg gagtgacgca tgcgtgtggc
    11281 cttcctcgtt gttgccgttc gacactcgac aatattagcg aattcttaac cagagcctta
    11341 tattcgaaag gagcgaatta ttcgccggcg cggcggtggc ggcatggggg gaaacccggg
    11401 gccggacagc cgcgggggcc gattgccgct agcgcgcgac gtcgctctcg atcgcgatct
    11461 gcgtgcaaac ggcacggcag agggtcacga cgcggtcgtt gacgatgcgg tagtagatct
    11521 gcacgccgtc gcgccgcttg cccagcacgc cggcctggta cagcgtgttc aggtgctggg
    11581 acatattggg ctgcgtggtg tcgatctccg ccagcagttc gctcacgttc ttctcgccgt
    11641 tgcagaggct ggcgatgatc ttcaggcgca tcggcgccga catcacgcgg aaaatttcgg
    11701 cggcgagctc gaagacctca tccgattcga ctgccgagtc cgtgggtgcg gagttgttgg
    11761 agcgcttggg catggaatgc gtctgtcgtt gtcagggtat tgcgcgagtt tatcgccagg
    11821 caagaatata agccttaatc caacgttgca ggtgagggcg cgccctagga ggcaccctag
    11881 gagtctaggg agtccgtgcc gccgttgctc cggcctcgcg ctcgagccgc cacacctggt
    11941 acctcagggc cgcggcggcg cccgcgatca tggcgcccac ggcgacgaag ctcgtcaagc
    12001 tgagcgtgga gataccggac acgccttgcc cgatgctgca gcccatggcg gtcacgccgc
    12061 ctactcccat caggacagca cccccgagat ggtgggccag gtcgtcggtg ccgccgaagc
    12121 cctcccagcg gaagctgcgc tgcgccagcg aaaccgacag cgaacccgcg atgacgccga
    12181 acacggacac gatgcccacg ctcagtgact tgttgcggtc gctgtagaac atcagccagt
    12241 ccagcgtgta ggcgatcggc gagacgaaac tgagcgcttc ggcgcgtgtg gaattggtgg
    12301 cgaggaaggt ctcctgcaat gtttccggat gctcggcgat gtggccgacg tgcccgctga
    12361 tccaccacat ggccacgacg ccggccccca cgccggcgcc tgccagcaca ttggtgccgc
    12421 gcaggaactc tcgcccggcc agcgcccagg ccaccagcgc gccgccgatc acgagcgaca
    12481 gcgccaggcc ggcggcggag gcctttgccg cgaacgcctg cgccgcccag gcgggaaggc
    12541 tggcctgggc ttggaagtcg agggcgacgc ggtccaccgt ttccgttcgc cagaccgcgg
    12601 tcaagccctt caaggtggcg aaggcggcga cgccgagcac cacgaccacg accaccgcct
    12661 tcaggttgcc gccgcctacc cgcaccaggg ctttgctggc gcagccggag gcaaggacca
    12721 tgccgaagcc gaaaatgccg ccgcccgcca gcgccgacag ccaggtccag cgattcgagg
    12781 cgtagagcgt cttgtccggg ctgaccagtc cggcccaggc cagcaagccg aaaccgacca
    12841 cggcgacgcc ggcggccagg ccccactggc gcatgcgggt ccagtcgccc atggtcacga
    12901 catcgctgac ggcccccatg gtgcaaaaac cggtgcgctg tacgacggcg ccgaaggcca
    12961 tggatacgaa aaacgatgcc cagagaacct gttgggtgag caacgccact tcgacactcg
    13021 tcatggcgcg attctaggcg ggcggcccgc gcgcttcctg cggccggaag gagcggccca
    13081 gggcctgttc acactgtttt tgccagatgc gttgtgatca gaaggggtca gccgctaggc
    13141 gcggaacacg gcctgggttg cacacccagc cgtgttccgt aacaacgcgg atggcccctt
    13201 ctgatcgcaa cccgcaggga gcggggtgat aaccgcgcca ctccgcgttg cgctcctagg
    13261 caaggcggcc agccttgccg tcgtcccgcg ccttgattgg cgcggttctc accccactcg
    13321 cactggcaaa aacagtgtga acaggcccta ggacgattcc taggctgcct aggcatccgc
    13381 gaaggcgcga accggactcc gccgaggctt ttttcagccg cgtccgatac ctcaccgggc
    13441 cgataaccgc cacagtacac gcatgccaca gagcaactcc cccgtcagag tgtttctggc
    13501 cgatgattcg gccctgatcc gcgagcgcgt cgccgcgatg ctcgccgcgc gcgccatggc
    13561 tatcgtcggc catgccgaaa cgcctcaggg ttccatcgac ggcatcctgg ccgcgcgccc
    13621 ggacgtggtg gttctcgatg tccagctcga gggcggctcg ggcctgcagg tcctgcgggc
    13681 gatccgccag tccgaccccg acatcgcgtt cgttgttttc agcaacaact ccggccccgc
    13741 ctaccgcaag cgctatctgg gcgagggggc gctgcgcttt ctcgacaaga gcacggagtt
    13801 cgatcagctc gtcccggcgg tcgaaactgc atcgcaacac gcgaactgaa tcaaagcatc
    13861 cgaggaacaa tccatgtccg ccgccacgct cgaacccaac gtcagccgca tccgcatcat
    13921 gtccgccgcg ccctccgtgc aggcgcagcc cgccaccgcg gcgacaaccc agtgctcgac
    13981 ctgccacctg cgcgacctgt gcctgcccag cggcatggcg gcccccgacg tggatcgcct
    14041 ggacaacatg aagttcgcgc ggcgcaaggt caaggccggc cagacgctct accgcgaagg
    14101 cgaccgcttc cagtacatct atgcggtgcg taccggtacg ctgaagtcca gcctgatgct
    14161 ggccgacggc cgtgagcagg tgagcggctt tcacatcgcg ggcgaactgg tgggcctgga
    14221 cggagcggcg cagggccatc acgccagcgc caccatggcg ctggaagaca ccgacatctg
    14281 cgccattcct tacgcgcacc tcacggacct ggccgcgtcg gtcagcggca tgcagcatgt
    14341 gatcagccgc ttgatgagcc gcgagatcct gcgcgagcac agcctgatga tgctcctggg
    14401 cagcatgaac gccgacgagc gcctggccgc cttcctgctg aacctgtcgc agcgctatgc
    14461 ggcgcgcggc tactccgagc gcgagttcca cctgcgcatg agccgcgccg agatcggcag
    14521 cta
//
LOCUS       DASWIY010000067         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1897633, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000067 DASWIY010000000
VERSION     DASWIY010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897633"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VGA59_02585"
     CDS             <1..1416
                     /locus_tag="VGA59_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9718590.1"
                     /translation="TTRKFGGTGLGLAICKRLAELMGGEVGVESEPGKGSTFWFSARL
                     GIGATRGQLIPIPDLRGRRALVVDDNDHARAVIMDMLEGMTFLASEVASGPAAVDEVR
                     KAATEGRPYEVVYLDWRMPGMDGMETARRIRSLGLASPPLFLMVTAYGREEVLKEAEG
                     SGIQNVLNKPVSASILFDSTMNVLGARGGEPSRADSARGVADHRLAALRGARILLVED
                     NDINQQVARELLEDVGLAVEVADNGQVALDMARKAPYDLVFMDMQMPVMDGLTATREM
                     RKLQRLSSLPIVAMTANAMEQDRRKCMDAGMNDYLIKPIDPQDMRSILLRWVRPRRAA
                     AAPTPARQQAPADGEVSEDIPGLDTRLGLSRMMGKKSLYLAMLRRYAAGQRTVAQEMR
                     QALAAGDRATAERVAHTTKAVSGNIGATLVQDRAAALELAIRDSSGAAEIERLLEELE
                     APLHELLAVLASHLPAEAGHA"
     gene            1537..2169
                     /locus_tag="VGA59_02590"
     CDS             1537..2169
                     /locus_tag="VGA59_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718591.1"
                     /translation="MSQAHAGVQAAEIRHPLHAAGLTDLVNRLAQELAEARRALAAAT
                     QKLGSTSRTLEGRTQELTEARAALALLLATLDSTTDGILAMGYFGRAMHYNTRFIEMW
                     RIPADKLATLNDGALMAIQMSQVKDPAGFLADAQARKAHSDDEHRTTIEMTDGRIFEC
                     HVMPQRVRGKRVGSVTSFRDVTEQERLGRMISALEAELPREVAEARASSY"
     gene            complement(2286..3044)
                     /locus_tag="VGA59_02595"
     CDS             complement(2286..3044)
                     /locus_tag="VGA59_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9718592.1"
                     /translation="MNFPLITDPFFYAVAIPAVLLMGISKSGFGTGFGSLAVPLMALA
                     VTVPQAAAIMMPILFLMDVMGLAAFHRHFDYKLIRFLLPLGLVGTVIGTLSFKLLDAR
                     LVAGIVGVFTLIFLAQRLLFPPRPDSPHPPRWLGAILTVTSGFTSFVSHAGGPPVSAY
                     VIPMRLPPLVFTATMAAFFFAVNLSKWIPYAWLGLLDLRNMTTSLALLPFAPIGVVAG
                     VRLAHRIRPLLFYRLIYAGMFLTGCKLVWDGFVH"
BASE COUNT          595 a          997 c         1078 g          433 t
ORIGIN      
        1 accacgcgca agttcggcgg caccgggctg ggcctggcga tctgcaagcg gctggccgag
       61 ctcatgggcg gcgaggtggg cgttgaaagc gaacccggca agggcagcac cttctggttc
      121 agcgcccggc tgggcatagg cgccacccgg gggcaactga tccccatccc cgacctgcgc
      181 ggccggcgcg ccctggtggt ggacgacaac gaccacgcgc gcgccgtgat catggacatg
      241 ctcgagggca tgacgttcct ggccagcgaa gtcgcttcgg gaccggcggc cgtcgatgaa
      301 gtgcgcaagg ctgcgacaga aggccggcct tacgaagtgg tctatctcga ctggcgcatg
      361 ccgggcatgg acggaatgga aacggcgcgc cgcatccggt cgctggggct cgcgtcccca
      421 ccgctgttcc tgatggtgac tgcgtacggc cgcgaggagg ttctcaagga agccgaaggc
      481 tcgggcatcc agaacgtgct gaacaagccc gtgagcgcgt ccatcctgtt cgacagcacc
      541 atgaacgtgc tgggcgcgcg ggggggcgag ccgtcgaggg cggactcggc gcggggcgtg
      601 gccgaccacc gtttggcggc cctgcgaggt gcgcgcatcc tgctggtcga ggacaacgac
      661 atcaaccagc aggtcgcgcg ggaactgctg gaagacgtgg gcctcgcggt cgaggttgcc
      721 gacaacgggc aggtcgctct ggacatggcc cgcaaggcgc cttacgacct ggtcttcatg
      781 gacatgcaga tgccggtgat ggatggcctc accgcgacgc gcgagatgcg aaagctgcag
      841 cggctctcga gcctgcccat cgtggccatg acggccaacg ccatggagca ggaccgccgc
      901 aaatgcatgg acgccggcat gaacgactac ctgatcaagc ccatcgatcc gcaggacatg
      961 cggagcatcc tgttgcgatg ggtgcgcccg cgccgggcgg ccgccgcgcc gacgccggcc
     1021 aggcagcagg cgcccgcgga cggcgaagtt tcagaagaca tcccgggtct ggacacgagg
     1081 ctgggcctga gccgcatgat gggcaagaag tcgctgtacc tggccatgct gcgccgttac
     1141 gcggcgggcc agcggaccgt cgcgcaggag atgcgccagg cgctcgccgc cggtgaccgg
     1201 gcgacggccg aacgggtcgc gcacaccacc aaggcggtgt cgggcaacat aggcgcaact
     1261 ctggtgcagg accgggcggc cgccctggaa ttggcgatcc gggacagcag cggggcagcc
     1321 gagatcgaac ggctgctgga ggaactcgag gcgcccctgc acgaactgct ggcagtgctc
     1381 gccagccacc ttccagcgga ggcggggcat gcgtgatggc cgggccgggg cgattcacct
     1441 agcgcgcggt aagaaatccg cctaccgcat gcgagttaca gcatggtttg atgacgcaat
     1501 ggcgtcatgg cgctcgaatt acagagaggt ttcgagatga gtcaagcaca cgccggtgtc
     1561 caagcagcgg agatacggca tccgctgcac gccgccggcc tcaccgacct ggtcaatcgg
     1621 ctggcacagg aactggccga agcgcggcgg gcgctggccg ccgccaccca gaaactgggc
     1681 tccaccagcc gcacgctcga aggcaggaca caggaattga cggaggcgcg cgccgcgctg
     1741 gccctgctgc tggcgacgct ggactccacc acggatggca tcctggcgat gggctatttc
     1801 ggccgggcca tgcactacaa cacgcgcttc atcgagatgt ggcgcatccc ggcggacaag
     1861 ctcgcgaccc tcaacgacgg cgcgctgatg gctatccaga tgtcgcaggt caaggacccc
     1921 gcgggtttcc tggcggatgc gcaagcgcgc aaggcccatt ccgacgacga gcaccgcacg
     1981 accatcgaaa tgactgatgg acgcatcttc gagtgccacg tgatgcccca gcgcgtgcgc
     2041 ggcaaacgcg tgggctcggt gaccagtttc cgcgacgtga ccgaacagga gcgtctgggg
     2101 cgcatgatct cggcgctcga ggccgaactg ccgcgcgagg tcgccgaggc cagggcctct
     2161 agctactagt ccactgagtc aggcaaatag ccgttacaaa ccgctgccag gtgcccaacc
     2221 taggctgcgg tttgcgccta gattgcggcc cttctgacgc tcacgggggg cgctatttgc
     2281 ctgactcagt ggacgaaccc gtcccacacc agcttgcagc cggtcaggaa catgcctgcg
     2341 tagatcagcc ggtagaacag cagcggccgg atgcggtgcg ccaatctcac gccggccacg
     2401 acgccgatcg gcgcgaacgg cagcagggcc agcgatgtgg tcatgttgcg cagatccagc
     2461 agtcccagcc acgcataggg aatccatttg ctgaggttca ccgcgaagaa aaacgccgcc
     2521 atcgtggcgg tgaacaccag cggcggcagg cgcatcggga tcacgtaggc gctgaccggc
     2581 ggtccccctg cgtgcgacac gaagctggtg aagcccgagg tgaccgtgag gatggcgccc
     2641 agccaccggg gcgggtgggg actgtccggc cgcggcggaa agagcaggcg ctgcgccagg
     2701 aagatcagcg tgaacacccc gacgatgccc gccaccagcc tggcatcgag cagcttgaag
     2761 gaaagcgtgc cgatcacggt gcccaccagc ccgagcggca gcaggaaacg gatcagcttg
     2821 tagtcgaaat gcctgtgaaa cgccgccagc cccatcacat ccatcaggaa caggatcggc
     2881 atcatgatgg cggcggcctg cggcaccgtc acggccagcg ccatcaaggg cacagccagc
     2941 gagccaaagc cggtaccgaa gccgctcttg ctgatgccca tgagaagcac cgccggaatg
     3001 gccacagcgt agaagaaagg gtcggtgatc agcgggaagt tcaagggcgg tccgggcatt
     3061 ctgcggcgct tttggcgcga cgctattttc gccgcaggcc atc
//
LOCUS       DASWIY010000068         7778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1521896, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000068 DASWIY010000000
VERSION     DASWIY010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521896"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1653)
                     /locus_tag="VGA59_02600"
     CDS             complement(229..1653)
                     /locus_tag="VGA59_02600"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9718593.1"
                     /translation="MHCALHELSAPALIEAYRRRELSPVEVIRSVLGHIERWEHHLHA
                     LYLLRPELAMQQARASEARWLHGAPLGVLDGVPVTLKENIATLGDPIPLGTAACELVP
                     STADAPPAARIREAGGVMVAKTTMPDYGMLSSGLSSFHKLARNPWDLGVTPGGSSAGA
                     GAAAAAGYGPLHMGTDIGGSLRLPAGWCGIFTLKPSLGRIPIAPPYMGRAAGPMTRSV
                     ENAAWFMQVLSLPDTRDSMSLPFQDIAWGEFDEGVDKLKGLRIGLLLEAGCGLAVEPE
                     IRAAVEHAARLFERAGAVVAPMHPFMTQAMLDGMDHFWRLRSHVDMKNLAPQRKAKVL
                     PYIQQWADSAAGMDGEAAFKAYSQFHETRVATVNACRAFDFVISPTSPVPAFPAALPS
                     PTNDPLRPLEHIGFTVPFNMSEQPAASINCGYTAQGLPIGLQIVGQRFDDLGVLQVAR
                     AFELIRDDQRPWPQPPSEALHHVD"
     gene            complement(1657..3180)
                     /locus_tag="VGA59_02605"
     CDS             complement(1657..3180)
                     /locus_tag="VGA59_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9718594.1"
                     /translation="MMKRRTVLTHSATVAALAAVPLAVSQSALAQGKKDSIVLAMALE
                     PSPGLDPTGGAASSIAEVTLYNIYETLTKINSDGSVTPLLAEKWEISPDLKTYTFKLR
                     KGVKFQNGEPFNAQAVKFSFDRAGGEKSTNKDKRTFANLSTQVVDDSTVVILAKEIDP
                     DFPFLMGQATAIIVEPKSADTNATKPVGTGPYKLESWAKGSSITLSKWDGYRNAANVK
                     IRKATFRFISDPAAQAAALLSGDVDAFPRIGTRVVPQFKGNKRFQVILGGSRAKTILA
                     INSKKKPLDDVRVRRAILSAIDRKAVIDGAADGFGTPIGSHYVPGAEGFVDTTGVNPY
                     NPEKAKQLLAEAGVKTPLELTMTLPPPSYARQGGEVIVSQLAKVGITVKTQNVEWAQW
                     LSNTYGGPHNYDLTIVSHVEPFDLGNYAKPDYYWGYQSKAFSDLYGKIKTTANPKERA
                     KLLGDAQKLLAADAANGFLFQPQFPIVARKEVKGLWKDMPIFVNDLAALSWAPGSSA"
     gene            complement(3249..4121)
                     /locus_tag="VGA59_02610"
     CDS             complement(3249..4121)
                     /locus_tag="VGA59_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9718595.1"
                     /translation="MTTPMNAPLLQVENLVREYTMPRERLFEPPHKVHALNGVSFTIE
                     AGRSMGIVGESGSGKSTIARLVMALDKPTSGSVKLLGRDLHALPREQLRQARRDIQMV
                     FQDPYGSLDPRQTVERIVTEPLAAQGDTARGEQHRRASDALTSVGLRANDLGKYPHEF
                     SGGQRQRIAIARALITRPRLIVADEPVSALDVSVQAQVLNLMQDLQAEFGITYMLISH
                     DLAVVRHLCDDVAVLWQGRIVEQGPPERLFRAAEHPYTRALLEAVPKAEPAKRVRHRQ
                     AQQNPNATADQTRG"
     gene            complement(4386..5381)
                     /locus_tag="VGA59_02615"
     CDS             complement(4386..5381)
                     /locus_tag="VGA59_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9718596.1"
                     /translation="MPLLEVENLHVRLQTHRGPAYAVRDVSFSLERGDTLGLVGESGC
                     GKTMAVMALMGLLPENAEITGSIRFDGQELTTRTDAQMCEIRGNRIGMIFQEPMTALN
                     PVHSIGRQVAEPLRLHRGLSADAARKEAIALLDRVGIPDAARRIDAYPHQFSGGQRQR
                     VTIAMALACGPDLLIADEPTTALDVTIQQQILELISDLVAERGMALMLISHDLGVIAQ
                     NVARTLVMYGGSVVESGPTGAVFAHRMHPYTLGLFAARPGLQSQKGKKLATIAGSVPE
                     LVDLPPGCPFAGRCRFTIAECHVTVPPPVMIEPGHHARCIRLEAVAAAKREGVAA"
     gene            complement(5387..6262)
                     /locus_tag="VGA59_02620"
     CDS             complement(5387..6262)
                     /locus_tag="VGA59_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9718597.1"
                     /translation="MSTIPVATPPLLHGPGFWKRALGHRSFVIGAVLSVLLVLAAMLS
                     LVWTPHSPYEVDMGAKLLPPDARHWLGTDPYGRDVASLLLVGARASIMVGVIAVSIGL
                     VIGTAFGLLAAARRGWVEETIMRLADFGFAFPAILSAIMLTAVFGAGMVNAIIAIGIY
                     NIPTFARITRASANAVWSREFVLAARACGKGSFSITMEHVLPNILSVLIVQATIRFAI
                     AILAEAALSYLGLGTQPPQPSWGRMLSEAQSLLFQAPLLAVFPGVAIALAVLGLNLLG
                     DGLRDLFDPRLARKR"
     gene            complement(6405..7355)
                     /locus_tag="VGA59_02625"
     CDS             complement(6405..7355)
                     /locus_tag="VGA59_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9718598.1"
                     /translation="MSLFLFKRLITLIATLVGASLVVFLVLEILPGNAAQMLMGPDAS
                     PEAVKALAIKLGIDRPPIERYWNWISGMLVGDLGLSYAYSTPVAELVLERMTLTVPLA
                     LMAMTITSVLALVAGIYAAARHNKLGDVGVMGLSQIGIAIPNFWFAILLILLFSVHLG
                     WFSAGGFPGWDEGILPGVKALLLPAISLAVVQSAILARITRSAVLEVLREDFVRTARA
                     KGVSRRGAMWGHVLRNAMIPVITVMGLQFAELLAGTIVVENVFYLPGLGRLIFQSISN
                     RDLIVVRNCVMLLATMVVIVNFAVDLLYAVIDPRVKASDI"
     gene            7401..7703
                     /locus_tag="VGA59_02630"
     CDS             7401..7703
                     /locus_tag="VGA59_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEX9718599.1"
                     /translation="MALMITDECINCDVCEPECPNEAIYLGPEIYEIDPARCTECVGH
                     FDEPQCVQVCPVACIPVNPAHVESRETLWQKYQRLAEQARQAVTAPVSAGPAAKNP"
BASE COUNT         1286 a         2533 c         2614 g         1345 t
ORIGIN      
        1 cgatcataag gggccatccg cgttgttacg gaacacggct gggtgtgcaa cccaggccgt
       61 gttccgcgcc tagcggctga ccccttctga tcgcaacgca tctggcaaaa atagtgtgaa
      121 caggccctag gcgccacccg caaagtgtga aagcgaaggg tcggtgcgcg ggccaccgcg
      181 gaaccggctt tgccgggctg ccggcgcttc gggggcgggc tttcggattc agtccacatg
      241 gtgcagcgct tcggagggcg gctgcggcca tggccgctgg tcatcgcgta tcagttcgaa
      301 agcgcgcgcg acctgcagca ctcccaggtc gtcgaagcgc tggccgacga tctgcaaccc
      361 gatcggcagg ccctgcgccg tatagccgca gttgatcgac gcggcgggct gctccgacat
      421 gttgaaaggc acggtgaagc cgatgtgttc cagcggccgc agcgggtcgt tggtgggcga
      481 gggcagtgcc gccgggaacg ccggcaccgg cgaggtcggc gagatgacga aatcgaacgc
      541 gcggcaggca ttcaccgtgg caacgcgggt ctcgtggaac tggctgtatg ccttgaaggc
      601 ggcctcgccg tccatgcccg cggcgctgtc cgcccactgc tggatgtaag gaagcacctt
      661 ggccttcctt tgcggcgcca ggttcttcat gtcgacatgg gagcgcagcc gccagaaatg
      721 atccatgccg tcgagcatgg cctgggtcat gaaaggatgc atgggcgcca ccacggcgcc
      781 cgcccgttcg aacagcctcg ccgcgtgctc caccgccgcg cgaatctccg gctctactgc
      841 caggccgcag ccggcttcca gcagcaagcc gatacgcaag cccttgagct tgtccacgcc
      901 ctcgtcgaac tcgccccagg cgatgtcctg aaacggcagg ctcatgctgt cgcgcgtatc
      961 gggcagcgac agcacctgca tgaaccaggc tgcgttctcg acgctgcgcg tcatcggccc
     1021 ggcggcccgg cccatgtaag gtggtgcgat cgggatgcgg ccgaggcttg gcttgagtgt
     1081 gaagatgccg caccagccgg cgggcagccg cagcgagccg ccgatgtcgg tgcccatgtg
     1141 cagtggcccg tagccggcgg ccgcggccgc gccggcgccg gcgctggagc cgccgggcgt
     1201 cacccccagg tcccaggggt tgcgcgccag cttgtggaag ctcgacagcc ccgaagagag
     1261 catgccgtag tcgggcatgg tggtcttggc gaccatcaca ccgcccgctt cgcgaatgcg
     1321 tgccgccggc ggcgcgtcgg ccgtggaagg caccagctcg cacgccgcgg ttccgagcgg
     1381 tatcggatcg cccagcgtcg cgatgttttc cttgagggtg accggcacgc cgtcgagcac
     1441 gcccagcggc gcgccgtgaa gccagcgcgc ctcgctcgct cgcgcctgct gcatcgcgag
     1501 ttcgggccgc agcaggtaga gcgcgtggag gtggtgctcc caccgctcga tgtggccgag
     1561 caccgaccgg atgacttcga ccggcgagag ctcgcgccgt cggtaggcct cgatcagcgc
     1621 cggcgccgag agctcatgca acgcgcagtg catccgtcaa gcagacgagc ccggggccca
     1681 ggacagcgcg gcaaggtcgt tcacgaagat cggcatgtcc ttccacaggc ccttcacttc
     1741 cttcctggcg acgatcggga actggggctg gaagaggaaa ccattggccg cgtcggcagc
     1801 cagcagcttc tgggcatcgc ccagaagctt ggcgcgctct ttcggattgg ccgtggtctt
     1861 gatcttgccg tacaggtcgc tgaaggcctt cgactggtag ccccagtagt aatccggctt
     1921 ggcgtagttg ccgaggtcga agggctccac gtgcgacacg atggtgaggt cgtagttgtg
     1981 cgggccgcca tatgtgttcg acagccactg cgcccactcg acgttctgcg ttttcaccgt
     2041 gatgccgacc ttcgccagct gggacacgat gacctcgccg ccctggcgtg cataggaggg
     2101 cggcggcaac gtcatggtga gctccagcgg cgttttcacg ccggcttcgg ccagcagctg
     2161 cttggctttc tccgggttgt aggggttgac gccggtggtg tccacgaagc cttccgctcc
     2221 cggcacgtag tggctgccga tgggcgtgcc gaatccgtcc gctgcgccat cgatcacggc
     2281 cttgcggtcg atcgcgctga ggatggcgcg gcgcacacgc acgtcatcga gtggtttctt
     2341 cttgctgttg atggctagaa tggttttcgc gcgcgagccg cccaggatca cctggaaccg
     2401 tttgttgccc ttgaattgcg gaaccacgcg ggtgccaatg cgcgggaaag catcgacgtc
     2461 gccggacagc agtgccgcgg cctgagccgc cgggtcggag atgaagcgga aagtcgcttt
     2521 cctgatcttc acgttcgcgg cgttgcgata tccgtcccat ttgctcaggg tgatggacga
     2581 gcctttggcc cagctctcga gcttgtacgg gccggtcccc acgggtttgg tggcgttggt
     2641 gtcggcgctc ttgggttcca cgatgatggc ggtggcctgg cccatcaaga atgggaaatc
     2701 ggggtcgatc tccttggcca gaatcaccac cgtggagtcg tcgaccacct gggtcgagag
     2761 attggcgaag gtgcgtttgt ccttgttggt gctcttttcg ccgcccgcac ggtcgaacga
     2821 gaatttgacc gcctgcgcat tgaagggctc gccattctgg aacttcacgc ccttgcgcag
     2881 cttgaaggta taggtcttga ggtcgggcga gatttcccat ttttctgcca gcaggggcgt
     2941 gacgctgccg tcgctgttta tcttcgtcag cgtttcataa atgttgtaaa gggtgacctc
     3001 ggcgatcgaa gaggcggcgc ccccagtcgg atcgagtccg gggctgggct ccagggccat
     3061 cgccagcacg atcgagtcct tcttcccctg ggccagcgcg ctttgcgaaa ccgccagggg
     3121 cacggcggcc agggcggcca cggtcgccga atgcgtgagt acggtacgtc gcttcatcat
     3181 gatttttgac tccagactaa aaaacaccaa cgaaagttcg aaagcccctt atcggggcgc
     3241 atttagcatc atccccgagt ttgatcggcc gtggcattag ggttttgctg ggcctgccgg
     3301 tggcgcacgc gctttgcggg ttcggccttt ggcaccgcct ccagcagggc acgcgtgtag
     3361 ggatgttcgg cggcgcggaa caggcgctcg gggggtcctt gctccacgat gcgcccctgc
     3421 cagagcaccg ccacgtcgtc gcacaggtga cgcaccacgg ccaggtcgtg actgatcagc
     3481 atgtaggtga tgccgaattc cgcctgcagg tcctgcataa ggttgagcac ctgagcctgc
     3541 accgacacgt ccagtgcgct gaccggttcg tcggccacga tcaggcgcgg gcgtgtgatg
     3601 agggcccgtg cgatcgcgat gcgctggcgc tggccgccgg aaaactcgtg cgggtatttg
     3661 cccaggtcgt tggcgcgcag gccgaccgaa gtcagcgcgt cggacgcccg ccggtgttgc
     3721 tcaccccgtg ccgtgtcgcc ttgcgcggcc aggggctcgg tcacgatgcg ttccacagtc
     3781 tggcgcgggt cgagcgaacc gtagggatcc tggaacacca tctggatgtc gcggcgcgcc
     3841 tggcgcagtt gctcgcgcgg cagcgcatgc aggtcgcggc ccaacagctt gacgctgccc
     3901 gacgtgggct tgtccagtgc catcaccagg cgcgcgattg tcgacttgcc cgaacccgat
     3961 tcgccgacga tccccatgct gcggccggcc tcgatcgtga agctcacgcc gttcaaggcg
     4021 tggaccttgt gcggcggctc gaacagccgc tcccgcggca tcgtgtattc gcgcaccagg
     4081 ttttccactt gcagcagcgg cgcgttcatg ggtgttgtca ttgcgggcgt attcattggc
     4141 ctgtcattgc gggcgcattc atgagcctgc cattgttgct ggcgcattca ttagcttgtc
     4201 attgcgggct gggctcgcgg aacgcccggg gcgcgcagct gtccagtgca gcgagcccaa
     4261 tgccgggcct gcgcgatgca cgtaccgagg catgacgctc cggcccggcc ccgcaatcca
     4321 tggatgccgg atcgtagtcc ggtatgacag atcctcatat cgtagtccgg catgacaagc
     4381 gtcaatcatg ccgcgacccc ttcccgtttt gccgccgcca cggcctccag ccgtatgcag
     4441 cgcgcgtggt gccccggctc gatcatgacc gggggcggga ccgtgacatg gcattcggca
     4501 atggtgaagc ggcagcgccc cgcgaaggga caccccggtg gtagatcgac cagttccggc
     4561 acgctgccgg cgatcgtcgc gagtttcttc cctttctgcg actgcaggcc cggtcgggcc
     4621 gcgaacaggc ccagcgtata aggatgcatg cgatgcgcga acaccgcgcc ggtcgggccg
     4681 ctctccacga cgctgccgcc atacatgacc agcgtccggg ccacgttctg cgcgatcacg
     4741 cccaggtcgt gcgagatcag catcaaagcc atgccgcgct cggccaccag gtcgctgatg
     4801 agttcaagga tctgctgctg gatggtcacg tccagcgcgg tggtcggttc gtcggcgatc
     4861 agcagatcgg ggccgcaggc cagcgccatg gcaatcgtga cgcgctggcg ctggccgccc
     4921 gagaactggt gcggatacgc gtcgatgcgg cgggcggcat cgggtatccc cacacggtcc
     4981 agcagcgcga tcgcctcctt gcgggccgcg tcggcggaca agccccggtg cagccgcagc
     5041 ggctcggcca cctgacggcc gatgctgtgc accggattca gggccgtcat cggttcctgg
     5101 aagatcatcc cgatgcggtt gccgcggatt tcgcacatct gcgcatcggt cctggtcgtg
     5161 agctcctggc cgtcgaagcg gatgcttccc gtgatctcgg cgttctccgg caacaatccc
     5221 atgagcgcca tcacggccat cgtcttgccg cagccggact cgccgaccag gcccagggtg
     5281 tcgccgcgct cgagcgagaa gctcacgtcg cgaacggcat aggccgggcc gcggtgcgtt
     5341 tgcagccgca cgtggaggtt ctcgacttcg agcagtggca acgggcttac ctcttcctcg
     5401 ccaggcgtgg atcgaacaag tcgcgcaggc cgtcgcccag cagattcagg ccgagcacgg
     5461 ccagcgcgat cgccacgccg gggaagacgg ccagcagcgg cgcctggaac aggagcgact
     5521 gggcttcgct gagcatgcgg ccccaggacg gctgcggggg ctgcgttccc aggcccaggt
     5581 acgacagcgc cgcttccgcc aggatggcaa tggcgaagcg gatcgtggcc tgcacgatca
     5641 gcaccgacag gatgttgggc agcacgtgct ccatcgtgat gctgaacgag cccttgccgc
     5701 aggcgcgcgc tgccagcacg aactcgcgcg accagacggc gttggccgaa gcgcgcgtga
     5761 tgcgcgcgaa ggtcgggatg ttgtagatgc cgatcgcgat gatcgcgttg accatgccgg
     5821 cgccgaacac ggctgtgagc atgatcgccg agaggatggc cggaaaggcg aagccgaagt
     5881 ccgccagccg catgatggtc tcttccaccc agccgcgccg cgccgcggcc agaaggccga
     5941 aggccgtgcc gatgaccagg ccgatgctca ccgcgatgac gcccaccatg atggacgcgc
     6001 gcgcgcccac cagcaacagc gatgccacgt cccggccgta cgggtcggtc cccagccagt
     6061 ggcgggcgtc gggtggcagc agcttggcgc ccatgtcgac ctcgtagggc gagtgcggcg
     6121 tccacacgag cgacagcatg gcggccagca ccagcagcac gctcaggacg gcgccgatca
     6181 cgaagctgcg gtggcccagc gcgcgcttcc agaagcccgg cccgtgcagc agcggcggcg
     6241 tggcgacagg tatcgtgctc atatgagtga tccattgcgc tgcgcgccac ccgcgacgca
     6301 tgaattcgcg cggccagtgc gggggccacc gcggaaccgg ctttgccggg ccgctggtgg
     6361 cgcccccttg agggggaggc gccgaaggcg cttcgggggt gggttcatat atcgctggct
     6421 ttcacgcgcg ggtcgatcac ggcgtacagc aggtcgacgg cgaagttcac gatcacgacc
     6481 atcgtcgcaa gaagcatgac gcaattgcgc accacgatca ggtcgcggtt ggagatcgac
     6541 tggaagatca gtcgccccag gcccggcagg tagaacacgt tctcgaccac gatggtgccg
     6601 gcgagcagtt cggcgaactg caggcccatc accgtgatga ccgggatcat cgcgttgcgc
     6661 agcacatggc cccacatcgc cccgcgccgg gatacgccct tggcgcgtgc cgtgcgcacg
     6721 aaatcctcgc gcagcacctc cagcaccgcc gagcgcgtga tccgcgccag gatcgccgac
     6781 tgcaccacgg cgagcgagat cgccggcagc agcaaggcct tgacacccgg caggattcct
     6841 tcgtcccagc ccgggaagcc cccggccgag aaccagccca ggtgtaccga aaaaagcagg
     6901 atcagcagga tggcgaacca gaagttcggg atggcgatgc cgatctgcga caagcccatg
     6961 accccgacgt cgccgagctt gttgtggcgc gccgcggcgt agataccggc caccagggcc
     7021 aataccgagg tgatggtcat ggccatcagc gccagcggga ccgtgagcgt catgcgctcc
     7081 aggacgagtt cggccaccgg cgtgctgtag gcgtagctca ggccgaggtc ccccaccagc
     7141 atgccgctga tccagttcca gtagcgctcg atcggcgggc ggtcgattcc cagcttgatg
     7201 gcgagggcct tgacggcttc cggcgaagcg tcggggccca tcagcatctg ggccgcattc
     7261 ccgggcagga tttcaagcac caggaacacc acgagcgatg cccctaccag cgtcgcaatc
     7321 agggtgatca gccgcttgaa caggaatagg ctcatgaagc cgtcgataat gccagaagtg
     7381 aagcgcggga gacccttggc atggcattga tgattaccga cgagtgcatc aactgcgatg
     7441 tctgcgagcc cgagtgcccg aacgaggcca tttacctggg cccggaaatc tacgagatcg
     7501 atccggcccg gtgcacggaa tgcgtcggcc acttcgacga gccgcagtgc gtccaggtct
     7561 gccctgtcgc gtgcattccc gtcaacccgg cacatgtgga aagccgcgag acgctgtggc
     7621 agaaatatca gcggctcgcc gagcaggccc ggcaggcggt cactgcgcca gtgtccgccg
     7681 gcccggccgc caagaacccc taggctgccc gtcaagcggc cttcttcttc gctttcttct
     7741 ttttcttcgt ctgggtctgg cccggcttct tcggcgat
//
LOCUS       DASWIY010000069         5721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1523706, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000069 DASWIY010000000
VERSION     DASWIY010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523706"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VGA59_02635"
     CDS             <1..1169
                     /locus_tag="VGA59_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX9718600.1"
                     /translation="GVFKASTVFFPNVAAMRSRDWKQKIGYTYGLHGTPTTFTLEERI
                     AALEGGKHCILVPSGLAAIANVSMALLKSGDEVLIPDNSYGPTKALAEGELKNWGIAH
                     QFYDPMKPDDLRARIGPKTRLVWLEAPGSVTMEFPPLAQLAAVCRQRGVTSVLDNTWG
                     AGIAFNGFDLGEGEPRVSDSGADIVVQALTKYPSGGGDVLMGSIVTRDDKLHLQLKLT
                     HMRVGWGIGGNDVEALLRSLPTLPLRYRAQDTAARTLAAWLKSRPEIARLLHPAFEGS
                     AGYEHWRALCGADGQGDGLAAGGAAAGLFSVVFDPRYSVAQVDAFCDALKLFKLGYSW
                     GGPVSLVVPYDIASMRSAALGTWPHKGVLVRFSVGLEAVEDLRADLEQALGAVA"
     gene            1236..1412
                     /locus_tag="VGA59_02640"
     CDS             1236..1412
                     /locus_tag="VGA59_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718601.1"
                     /translation="MATPNYGYEKRQKELAKKRKKEDKLKAKAERKVGGPADSDSSQD
                     DAGSGNQPPAPTQR"
     gene            complement(1557..2615)
                     /gene="mnmH"
                     /locus_tag="VGA59_02645"
     CDS             complement(1557..2615)
                     /gene="mnmH"
                     /locus_tag="VGA59_02645"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEX9718602.1"
                     /translation="MSLHKVSAAEAIARLDEFSTILDARSEAEYCLDHLPGALNWPSL
                     NNEERQLIGTIYTQVSPFEAQKRGAALVAANIARHIEREVIDKPRSWQPLAYCWRGGK
                     RSGALALVLDQIGFRVGVLEGGYKAFRSAILAALPQLAARLDYRVVCGPTGAGKTRLL
                     HALAQAGAQVLDLEALASHRSSVLGMIPGQPQPTPKRFDTLVWDQLRRFDPARPVFVE
                     SESRKVGNLAVPDALIERMRVSPCLRLELSDDERVALLLEDYDFFVKDQALFCDRLAA
                     LTELRGKAVVQGWQAQVAAGNVENVVRELLLKHYDPGYASSIARNFSGYAKAKTIAPA
                     DRSAAAMAELAGAILREM"
     gene            complement(2612..3283)
                     /locus_tag="VGA59_02650"
     CDS             complement(2612..3283)
                     /locus_tag="VGA59_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX9718603.1"
                     /translation="MFAGRWSRRHFIAAALAGGYGLPAAAAAAGTILVVGDSLSAEYG
                     LKRGTGWVALLEKRLAAEKIEARVVNASISGDTTSGGRSRLGALLAQHKPDVVVIELG
                     GNDALRGLPLDMTQDNLAHMTRSAQQAGARVLLVGMQMPPNYGADYANRFARLYENVA
                     KAQKAALVPFLLKGVGDAAEPARFFQADRIHPREEAQPLILANVWPELRKLLGANGRV
                     RAARP"
     gene            3282..3953
                     /locus_tag="VGA59_02655"
     CDS             3282..3953
                     /locus_tag="VGA59_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9718604.1"
                     /translation="MSDPIISVEHVHKSVTDSTGTLDILRDIDFTLAPRETAAIVGAS
                     GSGKSTLLSIIAGLDTPTRGTVRLAGCDLFSIDEDERAALRAHKVGFVFQSFQLLGNL
                     TALENVMLPLELAGRKDSRKAAGEMMERVGLAARLGHYPKVLSGGEQQRVALARAFVV
                     QPAVLLADEPTGSLDFATGETVMELMFDLNREQGTTLVLVTHDTAIATRCERRITIEA
                     GRIAG"
     gene            4116..4850
                     /gene="rlmB"
                     /locus_tag="VGA59_02660"
     CDS             4116..4850
                     /gene="rlmB"
                     /locus_tag="VGA59_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX9718605.1"
                     /translation="MSSPKVLFGFHAVGVRLKTAPQSIIEIYVDPTRRDARMRQFLDR
                     VNEAGARVIEADGLRLAKLAGSHGHQGVAARVQAVPAAHSLDDLLDQVEGPPLLLVLD
                     GVTDPHNLGACLRVADGAGVHAVIAPKDHAAGINATVAKVASGAAETVPYFMVTNLAR
                     TLGELKERSIWCIGASDDAPKTLYQAELKGPVALVLGAEGTGMRQLTRKTCDELVSIP
                     MRGAVESLNVSVASGVCLYEALRQRS"
     gene            4847..5563
                     /locus_tag="VGA59_02665"
     CDS             4847..5563
                     /locus_tag="VGA59_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX9718606.1"
                     /translation="MNEQVRAWVRAARHIAVLTGAGMSAESGVPTFRDAQTGLWAQFD
                     PQQLATEEAFRSNPSRVWDWYAFRREMIAKVEPNAGHRALAAFAHRHPGRLTLITQNV
                     DGLHQRAGSPGVLALHGNIFEDKWLDAPKACCNADAAAAGSPPYCDRCGNMLRPDVVW
                     FGEALPLQALAAAEEAASACDLMLVVGTSGVVYPAAGLARTAAGKVVIINPEPTELDD
                     AVDAVLRGKAAQLLPQLLED"
BASE COUNT          949 a         1931 c         1947 g          894 t
ORIGIN      
        1 cgggggtctt caaggcgtcc acggtctttt ttccgaacgt cgcagcaatg cgctcgcgcg
       61 actggaaaca aaagatcggc tacacctacg gcctgcatgg aacgcccacc accttcacgc
      121 tggaggagcg catcgcggcg ctggagggcg gcaagcactg catcctggtg cccagcgggc
      181 tggcggccat cgccaatgtc agcatggcat tgctcaagag cggcgacgag gtcctgatcc
      241 ccgacaactc ctacgggccg accaaggcac tggccgaagg cgaattgaag aactggggca
      301 tcgcacacca gttttacgac ccgatgaagc cggacgatct gcgcgcgcgc atcggcccca
      361 agacccggct ggtgtggctg gaggcaccgg gctccgtgac gatggaattc cccccgctgg
      421 cgcagctggc cgccgtctgc cgccagcgcg gcgtgaccag cgtcctggac aacacctggg
      481 gcgccggcat cgcgttcaat gggttcgacc tgggagaggg cgaaccgcgc gtgtcggaca
      541 gcggggccga catcgtggtg caggcgctga ccaagtaccc cagcggcggc ggcgacgtgc
      601 ttatgggcag catcgtcacg agggacgaca agctgcacct gcaactcaag ctcacccaca
      661 tgcgcgtggg ctggggcatc ggtggcaacg atgtcgaggc cctgctgcgc tcgctgccga
      721 ccctgccgct gcgctatcgc gcgcaggata cggcggcccg cacgctggcg gcctggctca
      781 agtcgcggcc ggaaatcgcg cgcctgctgc atccggcatt cgaaggctcg gccggctacg
      841 agcattggcg cgcgctttgc ggtgcggacg gccagggaga cggcctggcg gccggcgggg
      901 ccgcggcggg cctgttctcg gtggtgttcg acccgcgcta cagcgtcgcc caggtggacg
      961 ctttctgcga cgcattgaag ctgttcaagc tcggctattc ctggggcggg ccggtcagcc
     1021 tggtggtgcc gtacgacatc gcgtccatgc gcagcgccgc gttgggcacc tggccccaca
     1081 aaggcgtgct ggtgcgcttt tcggtcgggc tggaggccgt ggaggacctg cgcgccgacc
     1141 tggagcaggc actgggcgcg gtggcttaga ccggaactac ccgggagggg gtttatcgct
     1201 tacattggcc ccattgacag agaaacggaa cgcctgtggc tacaccaaat tacggatacg
     1261 agaaacggca gaaagaactg gccaagaagc gcaagaagga agacaagctc aaggccaaag
     1321 ccgaacgcaa ggtgggcggt cccgccgaca gcgattcttc gcaggacgat gccggaagcg
     1381 gtaaccagcc gccagccccg acccagcgct gaactgtatt gctcccctcg cacggggctg
     1441 tatttctccc tcttcctctg ggagagggca atacagccgc atgccctcac cctaaccctc
     1501 tccctgaggg agaggggata gatccaccct ctcccagagg agaggggata gaaacatcac
     1561 atctcccgca ggatcgcacc cgccagctcg gccatagccg ccgccgaccg gtcggctggg
     1621 gcgatggttt tcgccttcgc atagccactg aagttgcgtg cgatcgatga ggcgtagccc
     1681 gggtcatagt gcttgagcag cagctcgcgc accacgtttt cgacgttgcc cgccgccacc
     1741 tgtgcctgcc agccctgcac caccgctttc ccccgcaact cggtcagcgc cgccagccgg
     1801 tcgcagaaca gcgcctggtc cttcacgaag aaatcgtagt cttccagcaa gagggcgacc
     1861 cgctcgtcat cggacagctc cagccgcagg caggggctga cccgcatccg ctcgatcagg
     1921 gcgtcgggta ccgccaggtt gcccactttc ctgctttcgc tttccacgaa caccggccga
     1981 gccgggtcga accggcgcag ctggtcccac accagcgtgt cgaagcgctt tggggttggc
     2041 tgcggctggc cggggatcat gcccagcacc gagctgcggt ggctcgccag cgcctccagg
     2101 tccagcacct gggcgccggc ctgggccagg gcgtgcaaca ggcgtgtctt gcccgcgccg
     2161 gtggggccgc agaccacgcg gtagtcgagc cgcgcagcca gctgcggcag cgccgcgagg
     2221 atggcactgc ggaacgcctt gtagccgccc tcgagcacgc cgacccgaaa gccgatctgg
     2281 tccagcacca gggccagcgc cccgctgcgc ttgccgccgc gccagcagta ggccagcggc
     2341 tgccagctgc ggggcttgtc gatcacctcg cgctcgatgt ggcgcgcgat gttggccgct
     2401 accagcgccg caccgcgctt ttgcgcctcg aacgggctga cctgcgtata gatcgtgccg
     2461 atgagctggc gctcctcgtt gttcagcgac ggccagttca gggcgcccgg caggtggtcc
     2521 aggcaatatt cggcttcgct gcgcgcatcg aggatggtgc tgaactcatc gagccgggca
     2581 atggcctcgg cggcagagac cttgtgcaga ctcacgggcg ggcggcccgg acccggccgt
     2641 tggcgccgag cagtttgcgc aactccggcc agacgttggc caggatcagg ggctgcgcct
     2701 cctcccgggg atgaatgcgg tcggcctgga agaagcgcgc cggctcggcg gcatcgccca
     2761 cgccctttag caggaagggc accagggctg ccttctgggc cttcgccacg ttttcataca
     2821 gacgggcgaa gcggttcgcg tagtcggcgc cgtagttggg cggcatctgc atgcccacca
     2881 gcagcacgcg ggcgccggct tgctgcgccg atcgcgtcat gtgcgcaagg ttgtcctggg
     2941 tcatgtccag cggcaagccg cgcaaggcgt cgtttccgcc caactcgatc accacgacat
     3001 ccggcttgtg ctgggcgagc agggcgccca ggcgcgagcg cccgccggac gtggtgtcgc
     3061 cgctgatgct ggcattgacc acccgggctt cgatcttttc cgcggccagg cgcttttcca
     3121 gcagcgccac ccatccggtg ccgcgcttca ggccatactc ggcgctcagg gagtcgccga
     3181 cgaccaggat tgtgccggct gcggcggccg ccgccggcag cccataacct ccagctaatg
     3241 cagccgcgat aaagtggcgg cggctccaac gtccagcaaa catgtcagat cccatcattt
     3301 cagtcgagca cgtccacaaa tcggtcaccg actcgacggg cacgctcgac attttgcgcg
     3361 atatcgattt caccctggcg cccagggaga ccgccgccat cgtgggagcc tcgggttcgg
     3421 gcaagagcac gctgctgtcg atcatcgctg gcctggacac gcccacccgc ggcaccgtgc
     3481 ggctcgccgg ctgcgacctg ttcagcatcg acgaggacga gcgcgccgcc ttgcgggcgc
     3541 acaaggtcgg ctttgtgttc cagagctttc agctgctggg caatctcacc gccctggaaa
     3601 acgtgatgct gcccctggaa ctggccggcc gcaaggattc gcgcaaggcc gccggcgaga
     3661 tgatggagcg ggtgggcctg gcggcgcgcc tgggccacta cccgaaggtg ctgtccggcg
     3721 gcgagcagca gcgggtggcc ctggcgcgcg ccttcgtcgt gcagcccgcg gtgctgctgg
     3781 ccgacgagcc caccggcagc ctcgactttg cgaccggcga aaccgtgatg gagctgatgt
     3841 tcgacctgaa tcgggagcag ggcacgacgc tggtgctggt gacgcacgac accgccatcg
     3901 ccacgcgctg cgagcggcgc atcacgatcg aggcggggcg catcgctggg tgaggctgcg
     3961 cccgctccgt tgtcatgccg ggctcgaccc ggcatccatg acttcgggag tcatggattg
     4021 cggctcaagg ccgcaatgac agtttgttgt catgccgggc gtgacccggc atccaggact
     4081 taaggggcca ccgggggcca cgggataatc ccccgatgtc atcacccaaa gtcctgttcg
     4141 gattccatgc cgtgggcgtg cgcctgaaga ccgcgccgca gtcgatcatc gagatctacg
     4201 tggatccgac ccgcagggat gcgcggatgc ggcaattcct cgaccgcgtc aacgaagccg
     4261 gcgcgcgggt catcgaagcc gacggcctgc gcctggccaa gctggccggc agccatggcc
     4321 accagggcgt ggcggcgcgg gtccaggccg tgcccgcggc gcactccctg gacgacctgc
     4381 tggaccaggt cgagggcccg ccgctgctgc tggtactgga cggcgtcacc gatccccaca
     4441 accttggcgc ctgcctgcgc gtggccgacg gcgccggcgt ccatgcggtg atcgccccga
     4501 aggaccatgc cgccggcatc aacgccacgg tcgccaaagt ggccagcggc gcggccgaaa
     4561 ccgtacccta cttcatggtc accaacctgg cgcgcaccct gggcgagctg aaggaaagaa
     4621 gcatctggtg cataggcgcc agcgacgacg cgcccaagac cttgtaccag gcggaactga
     4681 agggccccgt ggcgctggtg ctcggcgcgg aaggcaccgg catgcgccag ttgacgcgca
     4741 agacttgcga cgaactggtg agcatcccga tgcgcggggc cgtggagagc ctgaacgtgt
     4801 cggtggccag cggcgtgtgc ctgtacgagg cgctgcggca aagatcatga acgagcaggt
     4861 tcgcgcgtgg gtgcgtgccg cccggcacat cgcggtactg accggcgccg ggatgagcgc
     4921 ggagtcgggc gtgccgacct tccgcgatgc ccagaccggc ctgtgggcgc aattcgatcc
     4981 gcagcagctc gccacggagg aggcgtttcg ctccaacccc tcgcgcgtgt gggactggta
     5041 tgccttccgg cgcgagatga tcgccaaggt cgaacccaac gccggccaca gggcgctcgc
     5101 tgccttcgcg caccgccatc ccggccgcct gacgctgatc acgcagaacg tcgacggcct
     5161 gcaccagcgc gccggcagtc cgggcgtgct ggccctgcac ggcaacatct tcgaggacaa
     5221 atggctcgat gcgcccaagg cctgctgcaa cgccgacgcc gccgctgcgg gcagcccgcc
     5281 gtactgtgac cgctgcggca acatgctgcg gcccgatgtc gtgtggttcg gtgaggcatt
     5341 gccgctgcag gcattggcgg cggccgaaga ggcggcctcg gcgtgcgacc tgatgctggt
     5401 ggtcggaacg tcgggcgtgg tctatcccgc ggccggcctg gcccgtacgg cggccggcaa
     5461 ggtggtcatc atcaaccccg agccgaccga actcgatgac gcggtagatg ccgtgctgcg
     5521 cggcaaggcg gcgcaactgc tgccccaact tctggaggat tgagacatgc gttattcgtt
     5581 aattgccgtt gcgaccgcgg ccgtcgccct gttgatcgcg tcctgcacgc aggagcagca
     5641 gaacaagctg ggccgcgaca tccagaactg gaccggcacc aatggcgtgc tggaggtgta
     5701 cagcggcgac aaggtcgtgc g
//
LOCUS       DASWIY010000070         7837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2268814, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000070 DASWIY010000000
VERSION     DASWIY010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2268814"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..368)
                     /locus_tag="VGA59_02670"
     CDS             complement(147..368)
                     /locus_tag="VGA59_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX9718607.1"
                     /translation="MGIEWLKPGAFFGSILYALIGVVFFWLSFLAIDKLTPYNLWEEL
                     VDKQNVALGIVVGAMVLGISIIVAAAVHG"
     gene            complement(405..1901)
                     /locus_tag="VGA59_02675"
     CDS             complement(405..1901)
                     /locus_tag="VGA59_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEX9718608.1"
                     /translation="MATESSQRSYRAACPGCGAPVEFRSAQSAYAVCSFCRSTVVRHG
                     ETLSRLGRMAELFEDHSPLQLLATGHWQDKAFTLVGRLQYRAPSGAWTEWIALFDDGT
                     EGVLGEDNGAYVFSVPAAIARELPPASQFRVGATTAIDGNPFEVASNEQTVLVSAQGE
                     LPKLPAVGRPFAMVALRSAAGEVLSIDYGEQPPQLTRGRSVRLEDLRLAGLREESAKQ
                     EGARQFNCPHCGAPVDVALASSKSIACRSCHSLIDMSQGIGGELHHAQQEAPEEPLIP
                     LGTVGRLQGAQWQVVGYQHRVGHEPGDEDENFGWDEYLLYNRQRGFIFLVDTQEGWSV
                     VKPTTGAPKLSAGEQTATYLGTRYELKYSYEAQTTYVAGEFYWPVERGQKTFNRDFAK
                     GPNLLSMERTPREVTWSSGTTIASDAVAKAFRLEQKKELLKRSDAGPVSAAGGRIGCG
                     TIIMMFILILILLGVIRSCMDGGRGGSGFAPRTGGGSFGGYSGGGGHK"
     gene            complement(2012..3061)
                     /locus_tag="VGA59_02680"
     CDS             complement(2012..3061)
                     /locus_tag="VGA59_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX9718609.1"
                     /translation="MALMDFIKKQFIDIIQWTEDGEGTLAWRFPMRDMEIQYGASLTV
                     RESQMAVFVDEGRVADVFGPGMYKLTTQTIPVLTYLKNWDKLFESPFKSDVYFFSTRQ
                     QVDRRWGTPQPITIRDKEFGAVRLRAFGNYTYRIADPKVFHTSISGTRDIYTVDDLDG
                     QLRGMFLQHISDAIAESKIPFLDLAANQIEFAAALATELLAPFAAVGLKLEAVTVQNL
                     SLPEELQKVLDQRIGMGMVGGDMGRFMQYQTAQAIPEMAKSAGSGGGSLAGDAMGLGA
                     GIAMGQVLAQNLQQGLKAGSDQAAAQAAAAQGAAVRPEDVMATLEKLGELKSKGILTQ
                     EEFDAKKAELLKKLV"
     gene            3119..3367
                     /locus_tag="VGA59_02685"
                     /pseudo
     CDS             3119..3367
                     /locus_tag="VGA59_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(3529..4074)
                     /locus_tag="VGA59_02690"
     CDS             complement(3529..4074)
                     /locus_tag="VGA59_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718610.1"
                     /translation="MRSLTLFALIALFGAGTAAGADFTIRRLAPSAFPQLPPSIRQTL
                     EQRDCLVPQLWEGWNSKPHNVIRGAFLRKKQIDWAVLCSIAGRSTILVFPRGASQPAA
                     ELAAADDENFVQEVMPGRPGFSRAIGAVGRKHILEFYEAYGGPKPPPMDHQGINDAFV
                     EKASGVHYFYKGEWLELSGAD"
     gene            4220..6532
                     /locus_tag="VGA59_02695"
     CDS             4220..6532
                     /locus_tag="VGA59_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX9718611.1"
                     /translation="MPPTNAVNTDDKRAQLRQAALEYHEFPTPGKIAIHATKQLLNQH
                     DLALAYSPGVASPCEEIVKDPNAAFKYTSRGNLVAVITNGTAVLGLGDIGPLAAKPVM
                     EGKGVLFKKFAGIDVFDIEINEKHDLDKLVDVIAALEPTFGGINLEDIKAPDCFYVER
                     KLRERMKIPVFHDDQHGTAIVVGAAIINGLKVVGKDIKKVKLVTSGAGAAALACLGLL
                     LKLGMPRQNIFVTDLAGVVHAGRKELMDEDKIQFAQETPARTLGEIIEGADIFLGLSA
                     GGVLKPEMVKKMAASPLILALANPNPEITPEDVKAVRADAIMATGRTDYPNQVNNVLC
                     FPYIFRGALDSGATTITVEMEIAAVHAMADLAQAEQSDVVAAAYAGQQLAFGPEYLIP
                     KPFDPRLMMKIAPAVAQAAADSGVALRPVKDMDAYRDKLQSFVYASGTAMKPIFAAAK
                     NAPKKRVAFCEGEEERVLRACQIVVDEGIVRPTLIGRPKIIAERIEKFGLRLKEELDY
                     DIANVEQDYRYRDFWQTYHRMAERKGVTAQMAKIEMRRRLTLIGSMLLHKDEVDGMIC
                     GTWGTTPIHLHYVDQVIGRRPGVATYACMNGLLLPGRQVFLVDTHVNYDPTAAQLAEI
                     TVLAAEEMMRFGLKPKAALLSHSNFGSSNQPSAVKMRQTLDLLRVQAPWLEVDGEMHG
                     DVALDGAQRDLLMPHSTLTGDANLLVLPNMDAANISYNLLKTAAGGNISIGPVLLGAA
                     KPVHILTASATVRRIVNMAALTVADANVAR"
     gene            6775..7119
                     /locus_tag="VGA59_02700"
     CDS             6775..7119
                     /locus_tag="VGA59_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HEX9718612.1"
                     /translation="MATPLAQARWLPDRPATSQPATVQVAELPRQGRETYELIRQGGP
                     FPYGKDGAVFGNRERLLPAEKRGFYREYTVTTPGSRDRGARRIVCGGPPRTPYACYYT
                     ADHYASFRKIVE"
     gene            7169..7600
                     /locus_tag="VGA59_02705"
     CDS             7169..7600
                     /locus_tag="VGA59_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEX9718613.1"
                     /translation="MDAPLRRESSNDTPLKGVRTNIVQSIRAFRVQELQDAARALGQH
                     FLYANLANGQSKQEVLDLIAQQFMLPAHFGKNFDALYDCMTDPVHKSGPQPGFIVVLE
                     QIPANAKFDKEAREQLLDIFRDTADYWADRKIPFRCFYSFL"
     gene            7579..7788
                     /locus_tag="VGA59_02710"
     CDS             7579..7788
                     /locus_tag="VGA59_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718614.1"
                     /translation="MFLFFSVARSAHTSQAERANEAQGEATGISVAPPAESGEKMPTD
                     KLVDVSPLALRMSSPFNAGYWLAAA"
BASE COUNT         1433 a         2569 c         2515 g         1320 t
ORIGIN      
        1 ccgcatggcc gcgagacgcg gccattcctc gacctgtcca aaccgacgca ggtcggtttg
       61 gagccgcagg tctcggcccc cttgaggggg agagggcgaa gcccgagggg gcatcggccg
      121 caggccgctt cgggggggag tcatttctac ccgtgcacgg cggccgccac gatgatggag
      181 atgcccagca ccatcgcgcc caccacgatg cccagcgcga cgttctgctt gtccaccagc
      241 tcttcccaca agttgtatgg cgtcagcttg tcgatggcga ggaagctcaa ccagaagaac
      301 acgacgccga tcagcgcgta gaggatggag ccgaagaagg ccccgggttt cagccactca
      361 atgcccatgt tgctctccag aaaaaatcat gccgaccagg aacgctactt gtggccgccg
      421 ccaccggaat acccaccgaa cgagccgcct ccggtgcgcg gggcgaagcc cgagccgcca
      481 cgcccgccgt ccatgcagga cctgatcacc cccagcagga tcaggatgag gatgaacatc
      541 atgatgatgg tgccgcagcc gatgcgcccg ccggcggcgc tgacaggccc cgcgtcgctg
      601 cgtttgagca gctccttctt ttgctccagc cggaaagcct tggccacggc gtcgctggct
      661 atcgtggtgc cgctggacca ggtcacttcc ctgggcgtgc gctccatcga gagcaggttg
      721 ggacctttcg cgaaatcgcg attgaaggtc ttctggccgc gctccaccgg ccagtagaac
      781 tcgccggcca cataggtggt ttgcgcctcg tagctgtatt tcagctcgta cctggtgccc
      841 aggtaggtcg cggtttgttc gccagccgaa agcttcggcg cgcctgtcgt gggcttgacg
      901 acgctccagc cctcctgggt gtcgaccagg aagataaagc cgcgctggcg gttgtacagc
      961 aggtattcgt cccagccgaa attctcgtct tcgtcaccgg gctcatggcc gacgcgatgc
     1021 tggtacccca ccacctgcca ctgcgcaccc tgcagccggc cgaccgtgcc caacgggatc
     1081 agcggttcct ccggcgcctc ctgctgcgcg tggtgcagct cgccgccgat tccctggctc
     1141 atgtcgatca ggctgtggca cgagcggcag gcgatgctct tgctggatgc cagcgccaca
     1201 tccaccggcg cgccgcaatg agggcagttg aactggcgag cgccttcctg cttcgcgctc
     1261 tcctcgcgca ggccggccag ccgcaggtcc tccagccgca ccgagcggcc acgggtgagc
     1321 tgcgggggct gctcgccgta gtcgatgctc agcacttcgc ctgccgcgct gcgcagcgcc
     1381 accatggcaa agggccggcc caccgccggc agcttgggca actcgccctg ggccgagacc
     1441 agcacggtct gctcgttgga ggcgacctcg aacggattgc cgtcgatcgc ggtggtcgcg
     1501 ccgacccgga actgggacgc gggcggcagc tcgcgcgcta tggcggcggg aacggagaac
     1561 acataagcgc cgttgtcttc gcccagcaca ccctccgtgc cgtcgtcgaa cagcgcgatc
     1621 cactcggtcc aggcgccgct gggggcccgg tattgcagcc gcccgaccag cgtgaaggcc
     1681 ttgtcctgcc agtggccggt tgccagcagc tgcagcgggc tgtggtcctc gaacagctcg
     1741 gccatccggc ccaggcgcga gagcgtttcg ccatggcgca ccacggtgct gcggcagaag
     1801 ctgcacaccg cataggcgga ctgcgcactg cgaaattcca ccggcgcgcc gcagccgggg
     1861 caggcggccc ggtaagagcg ttgggaggat tcggtggcca ctgggctgct gggttgtcat
     1921 tgcggccttg agccgcaatc cagacggttg gtgcgccaac tatccggtct caaccccgga
     1981 cttgatccgg ggccgggatg acagccgttt ttcagaccag cttcttgagg agttcggctt
     2041 tcttcgcgtc gaactcctcc tgcgtcagga tgcccttgga cttgagttcg ccgagctttt
     2101 ccagcgtggc catcacgtcc tcgggcctca cggccgcccc ctgcgcggcc gcggcctggg
     2161 cggcagcctg gtccgagccg gccttgagcc cttgctgcag gttctgcgcc agcacctgcc
     2221 ccatcgcgat gccggcgccc aggcccatcg catcgccggc caggctgccg ccacccgagc
     2281 cggcgctctt ggccatttcg ggaatcgcct gcgcggtctg gtattgcatg aacctgccca
     2341 tgtcgccgcc gaccatgccc atgccgatgc gctggtccag caccttttgc agttcctcgg
     2401 gcagcgacag gttctgcacc gtgacggctt cgagcttcag gccgacggcc gcgaagggcg
     2461 ccaggagttc cgtcgcgagc gccgccgcga actcgatctg gttggccgcc aggtccagga
     2521 agggaatctt gctttccgcg atggcgtccg agatgtgctg caggaacatg ccgcgcagct
     2581 ggccgtccag gtcgtccacg gtgtagatgt cgcgcgtgcc ggagatgctg gtgtgaaaca
     2641 ccttcgggtc cgcgatgcgg taggtgtagt tgccgaaagc gcgcagccgc acggcgccga
     2701 actccttgtc gcggatcgtg atcggctgcg gcgtgcccca gcggcggtcg acctgctggc
     2761 gcgtgctgaa gaagtagacg tcgctcttga agggagactc gaacagcttg tcccagttct
     2821 tcaggtaggt gagcacggga atggtctggg tcgtcagctt gtacatgccc gggccgaaca
     2881 catcggccac ccggccctcg tcgacgaaca cggccatctg cgactcacgt accgtgagcg
     2941 acgcgccgta ctggatttcc atgtcgcgca tcgggaagcg ccaggccagg gtgccttcgc
     3001 cgtcttccgt ccactggatg atgtcaatga actgtttctt gatgaagtcc atcagtgcca
     3061 tgaacgctcc tcgggttggg tgtcggcgca tgataaagcc cgacctgccg gctgtatagg
     3121 caaccccgaa agcctccatt catgctcgca cggcgccggg cgcgccatga gccgcggcga
     3181 agccaggcgc cgcttcacgc tggccgatca ccgcgccgcg accgagggag tggagtgccg
     3241 caaggacaag gacgtgatcg acgagacacc ggccgcctac aaggacattg ccgccgtgat
     3301 gcgggcgcaa agcgacctgg tggaagtggt gcacacgctc aagcaggtcg tgtgcgtgaa
     3361 gggctgagcg cggattccgg ccgcctgggc cgcaggaacc cctccatcac cagccacccg
     3421 gacgcaatgg tgggcagaag cgccgcgccc gccagccgca accgacgagg gacgctcgtg
     3481 ccggcgaccc gtctcttttg ggcgagctga tggacaacgc caggagcttc aatcggcgcc
     3541 cgaaagctca agccattcgc ccttgtagaa gtaatggacg cccgaagctt tctccacgaa
     3601 tgcatcattg attccttgat gatccatcgg gggtggtttc gggccgccgt acgcttcgta
     3661 aaactcgagg atgtgtttgc gccccaccgc accgatcgcg cgcgaaaacc cgggccttcc
     3721 cggcatgact tcctgcacaa aattttcgtc gtcggccgcg gccagttcgg cggcaggctg
     3781 cgaagcgcct cgcgggaaca ccagaatcgt ggatcttccg gcgatcgagc agagcaccgc
     3841 ccaatcgatc tgctttttcc tgaggaaagc cccccgaatg acgttgtgcg gcttcgaatt
     3901 ccagccttcc cagagttgtg gaacaaggca gtccctctgc tccagcgtct gacggatgga
     3961 gggcggcagc tgagggaagg cgcttggcgc aaggcgcctg atcgtgaagt cggcgcctgc
     4021 agcggtgccc gcgccgaata gcgcaatcag tgcaaacagg gtgaggctac gcatggacgc
     4081 atcagttata ggcgatgccc gccgttcgct acgcatcatt gcgtactagg gttttcccgc
     4141 gcggggctac aatcagaggc tccccagaca acggccctgc ctgcaatcgc gtgggcagcg
     4201 ccccaggaga cattcagcca tgccccccac gaacgcggtg aataccgacg acaagcgcgc
     4261 ccaattgcgc caggccgctc tcgaatacca cgagttcccc acccccggaa agatcgcgat
     4321 ccacgcgacc aagcagctgc tcaaccagca cgacctcgcg ctggcctatt cgccgggcgt
     4381 ggcctcgccc tgcgaggaaa tcgtcaagga cccgaacgcg gccttcaagt acaccagccg
     4441 cggcaacctg gtggccgtca tcaccaacgg caccgccgtg ctgggcctgg gcgacatcgg
     4501 ccccctggcc gccaagccgg tgatggaagg caagggcgtg ctgttcaaga agttcgccgg
     4561 tatcgacgtt ttcgacatcg agatcaacga gaagcacgac ctcgacaagc tggtggacgt
     4621 gatcgcggcg ctggagccca ccttcggcgg catcaatctc gaggacatca aggcgcccga
     4681 ctgcttctac gtggaacgca agctgcgcga gcgcatgaag attcccgtgt tccacgacga
     4741 ccagcacggc acggccattg tcgtgggcgc ggcgatcatc aacggcctga aggtcgtggg
     4801 caaggacatc aagaaggtca agctcgtcac ttcgggtgcc ggcgccgccg cgctggcgtg
     4861 cctgggcctg ctgctgaaat tgggcatgcc gcgccagaac attttcgtca ccgacctcgc
     4921 cggcgtggtc catgcaggcc gcaaggagct gatggacgag gacaagatcc agtttgcgca
     4981 ggaaacgccg gcgcgcacgc tgggcgaaat catcgagggc gccgatattt tcctggggct
     5041 gtcggccggc ggcgtgctca agcccgagat ggtcaagaag atggcggcca gcccgctgat
     5101 cctggcgctg gccaacccga atccggaaat cacgccggag gacgtcaagg ccgtgcgcgc
     5161 ggacgcgatc atggccaccg gccgcaccga ttacccgaac caggtcaaca acgtcctgtg
     5221 cttcccctat atctttcgcg gcgcgctcga ctcgggcgcg accaccatca ccgtcgagat
     5281 ggagatcgcg gccgtccatg cgatggccga tctggcccag gccgagcaga gcgacgtggt
     5341 ggctgcggcc tacgcgggcc agcagctggc gttcggcccg gaatacctga ttcccaaacc
     5401 gttcgatccg cgcctgatga tgaagatcgc gccggccgtg gcccaggcgg cggccgacag
     5461 cggcgtggcg ctgcgaccgg tcaaggacat ggatgcctac cgcgacaagc tgcaaagctt
     5521 cgtctatgcc tcgggcacgg ccatgaagcc catcttcgcg gcggcgaaga acgccccgaa
     5581 gaagcgggtg gccttctgcg aaggcgaaga agagcgggtg ctgcgcgcct gccagatcgt
     5641 ggtggacgag ggcatcgtgc gtccgacgct gatcgggcgg ccgaaaatca tcgccgagcg
     5701 catcgagaag ttcggcctgc gcctgaagga ggagctcgat tacgacatcg ccaacgtcga
     5761 gcaggattac cgctaccgcg acttctggca gacctaccac cgcatggcgg aacgcaaagg
     5821 cgtgacggcg cagatggcca agatcgagat gcgccggcga ctcacgctga tcggttcgat
     5881 gctgctgcac aaggacgagg tggatgggat gatctgcggc acctggggca cgacgccgat
     5941 ccatctgcac tatgtcgacc aagtgatcgg ccggcgcccg ggcgtcgcca cctatgcgtg
     6001 catgaacggg ctgttgctgc ccggccgcca ggtgttcctg gtcgacaccc acgtcaacta
     6061 cgaccccacc gccgcgcagc tggccgagat caccgtgctg gcggccgagg agatgatgcg
     6121 cttcggcctc aagccgaagg ccgcgctgct gtcccattcc aacttcggca gcagcaacca
     6181 gcccagcgcc gtgaagatgc gccagacgct ggacctgctg cgcgtgcagg cgccctggct
     6241 ggaagtggac ggcgagatgc acggtgatgt ggcgctggac ggcgcgcagc gcgacctgct
     6301 catgccgcac agcaccctga ccggcgacgc caacctgttg gtgctgccca acatggacgc
     6361 agccaatatt tcttacaacc tgctcaagac cgcggccggc ggcaatatct cgatcgggcc
     6421 ggtcctgctg ggcgcggcca agccggtgca tatcctcacc gcaagcgcga ccgtgcggcg
     6481 catcgtcaac atggcggcat taaccgtcgc cgacgccaac gtggcaagat aaggccgttt
     6541 tagacagctg gcactaactt attgaccagg tcaggacccg gcaatagcca tgccggctgc
     6601 ccaaaaattg agcagccgct tgccttttgg gaccagataa gtcacactac cgccttgaaa
     6661 ttttccgggt ttacccggag tgtggcgtcc cccaaaggtc accgaagaag gtccgtcatg
     6721 gcgcgcatcg cggctgccaa gtttgcgctt acttgctatt tgttggcagc agcgatggcg
     6781 acgcccctgg cgcaagccag gtggttgccg gaccgccccg ccacgagcca gccggccacc
     6841 gtccaggttg ccgagttgcc caggcagggc cgggagacct atgaactgat ccgccagggc
     6901 ggaccgttcc cgtatggcaa ggacggcgcg gtgttcggca accgggagcg cttgctgccg
     6961 gctgaaaagc gcgggtttta ccgcgaatac accgtgacca caccaggatc acgcgaccgg
     7021 ggtgcccggc gcatcgtgtg cggcggtcct cccaggactc cgtacgcctg ttattacacc
     7081 gctgaccatt acgccagttt caggaagatt gtggagtgag ccctaaatga agcgggacct
     7141 gtttgtgact atggaaagag aggcggagat ggatgcacca cttcgtcggg aaagcagcaa
     7201 cgacacgccg ctcaagggcg tgcgcaccaa catcgtgcag tcgatccggg ccttcagggt
     7261 ccaggaactg caggacgccg cgcgcgcgct ggggcagcat ttcctctatg ccaatctggc
     7321 caacggccag agcaagcagg aggtgctgga cctgatcgcg cagcagttca tgctgccggc
     7381 gcatttcggc aagaacttcg acgcgctgta cgactgcatg accgacccgg tccacaagtc
     7441 gggcccgcag cccggcttca tcgtggtgct ggagcagatt ccggccaacg ccaagttcga
     7501 caaggaagcc cgcgagcagc tgctcgacat tttccgggat actgccgact actgggcgga
     7561 tcgaaaaata ccgttcagat gtttctattc ttttctgtag cccgttctgc acacaccagc
     7621 caagcagaac gggcgaacga ggctcaggga gaggcgaccg ggatcagcgt tgcgcctccc
     7681 gcggaaagcg gcgagaaaat gccgaccgac aaattggtgg acgtatcgcc gctggcgctg
     7741 cgcatgagca gccccttcaa cgcgggatat tggttggcag cagcttagga agggctgtca
     7801 tcccgggctt gacccgggat ccatgaattc ggagttc
//
LOCUS       DASWIY010000071         5982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3008876, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000071 DASWIY010000000
VERSION     DASWIY010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3008876"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VGA59_02715"
     CDS             <1..351
                     /locus_tag="VGA59_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEX9718615.1"
                     /translation="SLMRDAFLSWDEEFVLDITVRYWQRALKAGLPVDPDFGEFWRAV
                     EWMGLQRHLKVAGIFARLTLRDGKPRYLADTPRFIAYIRATAGRYRELAPLLRLIDEV
                     EGTQAVSGYAFGRI"
     gene            362..1099
                     /locus_tag="VGA59_02720"
     CDS             362..1099
                     /locus_tag="VGA59_02720"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX9718616.1"
                     /translation="MPRFHCAAPLVTGQSLDLPAAAARHVQVLRLQPGARITLFNGAG
                     GEFEAVVEHMGRSEVRVQVAQHHAVEREARREVHLVVGMPANERMDWLVEKATELGAA
                     SIQPLVAQRSVLKLKGERAGRKQAHWQGIAVAACEQCGRNRVPAVREVLGLDEWLAGV
                     SRPSIDGQASHARAVRLVLSLASEARPLPAAASGDAPVSLLSGPEGGFAAAEEAAALA
                     AGYARVTLGPRVLRAETAPLAALSVLA"
     gene            1168..2337
                     /locus_tag="VGA59_02725"
     CDS             1168..2337
                     /locus_tag="VGA59_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9718617.1"
                     /translation="MKKNLGLLAACQGLYLTNNVTFIAINGLVGLALAPLGWMATLPV
                     MAYVVGGALSTKAVAITQERFGRKASFQAGLAVALASALLCAYAAVSRNFWLLCFATL
                     VAGYYNANAILYRFAAAELATPAAREKAVSLVLAGGLLGAVIGPNLAARTRDLSSVPF
                     VGAYLALAGVALLAMLLMLFIDFPPTPVRTSSHDGKPLAEIMRQPVFIVAAISGALGY
                     GVMNLLMAATPIAMQQYSLPFSDAAFVLQWHVIGMFAPGFFTGNLIKRFGPLPVMGVG
                     VALNLACILVALSGVEVHQFLVSLFLLGVGWNFLFTGSTTLSLSAYGPQERDRAQGAL
                     NFFVFATMAVSSLASGVLVTTQGWTLLNYGSLVPVALTGAALLWLGRKTARQATA"
     gene            complement(2249..3160)
                     /locus_tag="VGA59_02730"
     CDS             complement(2249..3160)
                     /locus_tag="VGA59_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9718618.1"
                     /translation="MSREVSESTLSTFTASDGDNIALQDWPLPEGVTLRGVVLIVHGL
                     GEHAGRYGHVASRLNEWGFAVRGYDQYGHGESDGVRGGLPTTARLMDDLADVIESTRA
                     RMEPGLPLILLGHSMGGLVTACHVALRGVPVEGLVLSSPALDAGLNSLQRLLLATMPR
                     LMPNLTVGNGLDADFISHDPDVVAAYKADRAVHDRISGRLARFIADGGPLVLAAASEW
                     KVPTLLMYAGADKLVHPRGSRAFAAAAPSEVVTVRCFDDLFHEIFNELDAEPVFETLR
                     QWLDERFSAPATAGPLPSRRPEPASRN"
     gene            complement(3241..4713)
                     /locus_tag="VGA59_02735"
     CDS             complement(3241..4713)
                     /locus_tag="VGA59_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX9718619.1"
                     /translation="MNAPLHREIPAGVLDPAAALSQVTEKWDRDIVRQLTEYIAIPAK
                     SPAFDPDWVRHGYIETVVRNAAAWVEAQKVEGLSLEIVRLPGRTPVLFFEIAATRTAS
                     SQTVLMYGHLDKQPEFTGWRSDLGPWTPKYEDGKLYGRGGADDGYAVYASIAAVQALK
                     AQQVRHPRIVGLIETCEESGSYDLLPYIDALRPRLGEVALVICLDSGAGNYDQLWLTT
                     SLRGMASGTLKVEILAEGVHSGDASGLVPSSFRIMRQVLDRLEDSKTGRLLPASFHCE
                     VPPERLAQAKATAAILGEEVYKRFPWAHYDCGGSTAFALPTTTDPLQALINRTWIPTL
                     SVTAADGFPSLQDAGNVLRPYTAFKLSLRLPPLVDATGAVRQLKALLEDHAPYQAKVT
                     FEAGSSAAGWSAPDTAPWFEQALNQASQAHFGAACGYIGQGGTIPLMNMLSTGFPRSQ
                     MMVCGVLGPRSNAHGPNEFLDVPYAKKLTAAVAQVVAQLP"
     gene            4796..5611
                     /locus_tag="VGA59_02740"
     CDS             4796..5611
                     /locus_tag="VGA59_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9718620.1"
                     /translation="MSISARDPSESGESRPLHVEAGEHRRIHVGVGGWTYEPWRANFF
                     PAGLPHSQELAYASRRLTAIEVNGTYYSTFKPPTFAKWRDETPDGFVFSLKANRFATN
                     RKLLATAAESIERFVGSGITELGDKLGPLVWQFMPTKQFEPEDFEAFLALLPKSVEGR
                     ALRHVLDVRHESFRSPDYLPLARRYGCATVHTDSDKFPAIADAESDLAYIRLMRSAAD
                     CETGYPPEVLGQWAQGAQAWTGAAPAREVFIFLINGAKERAPAAAMELIQRLS"
     gene            5761..5952
                     /locus_tag="VGA59_02745"
     CDS             5761..5952
                     /locus_tag="VGA59_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEX9718621.1"
                     /translation="MAYVFETVPADRGLIDLDDPREVNWWSKQFGCSEEQLRQAVEKV
                     GRSAAKVEQLLDGHDILSV"
BASE COUNT          976 a         1981 c         2024 g         1001 t
ORIGIN      
        1 agcctgatgc gcgatgcctt cctcagctgg gacgaggaat tcgtgctcga tatcaccgtg
       61 cgctactggc agcgcgcgct caaagccggc ctgccggtcg acccggattt cggcgagttc
      121 tggcgcgcgg tggaatggat gggcctgcag cgccacctca aggtggccgg tatcttcgcg
      181 cggctgaccc tgcgcgacgg caagcccagg tacctggccg acacgccgcg gttcatcgcc
      241 tacatccgcg cgacggccgg ccgttaccgc gagctcgcgc ccttgctacg gctgatcgac
      301 gaggtcgaag gcacgcaggc cgtctccggc tatgcgttcg gccggatctg accggcagcg
      361 gatgccgcgc tttcactgcg ccgcgccgct ggtcacgggc caatcgctgg acttgcccgc
      421 ggcggcggcc cggcatgtgc aggttctgcg cctgcagccg ggcgcgcgta tcaccctgtt
      481 caacggcgcc ggcggcgaat tcgaggcggt ggtcgagcac atgggacgca gcgaggtgcg
      541 ggtacaggtg gcacagcatc acgccgtgga acgggaagcg cggcgcgaag tgcatctggt
      601 ggtgggaatg cccgccaacg aacggatgga ctggctggtg gagaaagcga ccgaactggg
      661 ggcggccagc atccagccgc tggtggcgca gcgcagcgtg ctgaagctca agggcgaacg
      721 ggccggcagg aagcaggcgc actggcaggg cattgccgtc gccgcctgcg agcaatgcgg
      781 gcgcaaccgc gtgccggccg tgcgcgaagt actgggattg gatgagtggc tggccggcgt
      841 gtccaggccg tcgatcgatg ggcaggcaag ccatgctcgc gccgtgcgat tggtgctttc
      901 gctggcgagc gaggcgcgcc cgctgccggc cgccgcgtcc ggcgatgcac cggtcagcct
      961 gctgtccggg cccgaaggtg gtttcgcggc cgccgaagaa gcagcagcgc tggcggcggg
     1021 ctacgcccgc gtcactttgg ggccgcgtgt gctgcgcgcc gagaccgcgc cgctggcggc
     1081 gctgagcgtg ctggcctgac gggccgcatc cgccggcagc ggcaagcagt cgattgaaga
     1141 atgccgcaga atgacgccca gccagtcatg aaaaaaaatc tgggcctgtt ggccgcctgc
     1201 caggggctct atctcaccaa caacgtcacc ttcatcgcca tcaatggcct ggtggggctg
     1261 gccctcgctc cgctgggatg gatggcgacg ctgccggtga tggcctacgt ggtgggcggc
     1321 gccttgtcca ccaaagcggt cgcaataacg caggagcgct tcgggcgcaa ggcctcgttc
     1381 caggcgggcc tggcagtcgc gctggcgtcc gcgctgctgt gcgcctatgc ggccgtcagc
     1441 cggaatttct ggctgctgtg cttcgcgaca ctggtcgccg gctattacaa cgccaacgcc
     1501 atcctgtacc gcttcgcggc cgccgagctg gccacgcccg cggcgcgcga gaaggccgtg
     1561 tcgctcgtgc tggccggcgg actgctgggc gcggtgatcg gtcccaatct tgcggcgcgc
     1621 acccgcgacc tttccagcgt gccattcgtg ggcgcttacc tcgcgctggc cggcgtggcg
     1681 ctgctggcga tgcttctgat gctgttcatc gacttcccgc caacgccggt gcgcacctca
     1741 tcgcatgacg gaaaaccgct cgccgagatc atgcgccagc cggtcttcat cgtggcggcc
     1801 atctcgggcg ccttgggcta cggcgtgatg aacctgctga tggccgcgac accgatcgcg
     1861 atgcagcagt acagcctgcc gttctccgat gcggctttcg tgctgcagtg gcatgtgatc
     1921 ggcatgttcg cccccggctt cttcaccggc aacctgatca agcggttcgg gcccctgccc
     1981 gtgatgggtg tcggtgtggc cctgaacctc gcctgcatcc tggtggcgct gtcgggcgtg
     2041 gaggtgcacc agttcctggt gtcgctgttc ctgctgggcg tcggctggaa cttccttttc
     2101 accggcagca ccacgctgtc gctgtcggcc tacggcccgc aggaaaggga ccgcgcccag
     2161 ggggcgctga acttcttcgt gttcgccacg atggccgtga gttcgctcgc gtccggggtg
     2221 ctggtcacca cccagggctg gacgctgctc aattacggct cgctggttcc ggtcgccttg
     2281 acgggagcgg ccctgctgtg gctggggcgg aaaaccgctc gtcaagccac tgcctgagcg
     2341 tttcgaacac gggctcggca tcgagctcgt tgaagatttc gtggaagagg tcgtcgaagc
     2401 aacgcacggt caccacttcc gaaggtgccg cggccgcgaa cgcccggctg ccgcggggat
     2461 ggaccagctt gtcggcaccc gcatacatca gcaatgtcgg caccttccac tccgaagcgg
     2521 cggccagcac cagcggcccg ccgtcggcga tgaaccgcgc caggcgcccc gatatgcgat
     2581 cgtgcactgc acggtccgcc ttgtatgcgg ccaccacgtc ggggtcgtgc gagatgaaat
     2641 cggcgtcgag tccattgccc accgtgaggt tgggcatgag gcgcggcatg gtggcgagca
     2701 gcaatctctg caaagagttc agtcccgcgt ccagtgccgg cgacgaaagc accaggcctt
     2761 cgaccggaac cccgcgcaac gccacgtggc aagcagtcac caagcccccc atgctgtgcc
     2821 ccagcaggat gagcggcagg ccgggttcca tccgggcgcg cgtgctctcg atcacgtcgg
     2881 ccaggtcgtc catcaaccgc gccgtcgttg gcagcccgcc gcgcacgccg tccgattcgc
     2941 cgtggccata ctggtcgtag ccgcgcacgg cgaagcccca ttcgttcaac cgggaagcca
     3001 cgtggccata gcgcccggcg tgctcaccca atccatgcac gatcagcacg accccgcgca
     3061 gggtcacgcc ttcaggcagt ggccagtcct gcaaggcgat gttgtcgccg tcactggcgg
     3121 tgaaggtgga cagggtggat tcgctgactt cccggctcat agcggaccga tgatggggcg
     3181 ggtaactcaa cagcgcaatg gatcgcgccc aaacctgtgc ggtagttcac aaattccgtt
     3241 ctacggcagc tgcgcgacga cctgggcgac ggcggccgtc agcttctttg catacggcac
     3301 atccaggaat tcgttggggc cgtgcgcgtt gctcctggga ccgagcacgc cgcagaccat
     3361 catctgcgaa cgcgggaaac cggtgctcag catgttcatc agcggaatcg tcccgccctg
     3421 cccgatgtag ccgcaggccg cgccgaagtg cgcctgcgat gcctggttca gcgcctgctc
     3481 gaaccagggc gcggtgtccg gcgcgctcca gccggcggcc gagctgccgg cttcgaaggt
     3541 gaccttggcc tggtagggcg cgtggtcctc gagcagggcc ttgagttggc gcaccgcccc
     3601 ggttgcgtcc accagcggcg gcagccgcag cgacagcttg aaggcggtgt acggccgcag
     3661 cacgttgccc gcgtcctgca gcgaaggaaa gccgtcggca gcggtgaccg ataacgtcgg
     3721 tatccacgtg cggttgatca acgcctgcaa cggatcggtg gtggtgggca gcgcgaacgc
     3781 ggtggagccg ccgcagtcgt aatgcgccca tggaaagcgc ttgtacacct cctcgccgag
     3841 gatcgccgcc gtggccttcg cctgcgccag ccgctcgggc ggaacctcgc aatggaagct
     3901 ggcgggaagc agccggccgg tcttgctgtc ctccagccgg tccagcacct gccgcatgat
     3961 gcgaaagctc gaaggcacca ggcccgacgc gtcgccggaa tgaacaccct cggccaggat
     4021 ctccaccttg agcgtgccgc tggccatgcc gcgcaacgag gtcgtgagcc agagctggtc
     4081 gtagttgccg gcgcccgaat ccaggcagat caccagcgcc acctcgccca accgcgggcg
     4141 cagcgcgtcg atgtagggca gcagatcgta tgaacccgat tcctcgcagg tctcgatcag
     4201 acccacgatg cgcggatgcc gcacctgctg cgccttgagc gcctgcacgg cggcgatgct
     4261 ggcgtatacc gcatagccgt catcggcgcc gccgcgccca tagagctttc cgtcctcgta
     4321 cttcggcgtc cagggaccga ggtcgctgcg ccagccggtg aattccggct gcttgtccag
     4381 gtggccgtac atcaggacgg tctggctgga cgcagtgcgg gtcgccgcga tttcgaagaa
     4441 cagcaccggg gtgcgcccgg gcagtcgcac gatctcgagg gacaggcctt cgaccttctg
     4501 cgcttcgacc caggccgcgg cattgcgcac caccgtttcg atgtagccgt gccggaccca
     4561 atcggggtcg aaagccggcg acttcgccgg gatcgcgatg tactcggtta gctggcggac
     4621 gatgtcgcga tcccacttct cggtgacttg ggacagggcg gcggcggggt cgagcacgcc
     4681 agcgggaatc tcacggtgaa gcggagcgtt catgtcagta gtgctggcgg ggaaaagcgc
     4741 cactgtagcg ccactgaata ttgcccgcgt aaagtatgtg tattggaggc aactgatgag
     4801 catctccgca cgcgatccca gcgaatccgg cgaaagccgt ccgttgcacg ttgaagccgg
     4861 cgagcatcgt cgtatccacg tcggcgtggg cggctggacc tacgagccgt ggcgcgccaa
     4921 cttctttccg gcgggactgc cccacagcca ggagctcgcc tatgccagcc gccggctcac
     4981 ggcgatcgag gtcaacggca cttactacag cactttcaag ccgcccacat tcgccaaatg
     5041 gcgcgacgaa acgccggatg gtttcgtgtt ctcgctcaag gccaaccggt tcgccaccaa
     5101 ccgcaagctg ctggccacgg cggccgagtc gatcgagcgc ttcgtcggca gcggcatcac
     5161 cgagctgggc gacaagctcg ggccgctggt ctggcagttc atgccgacca agcagttcga
     5221 gccggaggat ttcgaggcct tcctcgcgtt attgcccaag agcgtcgaag gccgcgcgct
     5281 gcgccacgtg ctcgacgtgc ggcacgagag ctttcgctcg cccgattacc tgcccctggc
     5341 gcgccgctat ggctgcgcca cggtgcatac cgactccgac aagttccccg cgatcgccga
     5401 cgcggagagc gacctcgcgt acatccggct catgcgcagc gccgccgact gcgagaccgg
     5461 ctatccgccc gaagtgctcg gccagtgggc acaaggcgcg caggcctgga ccggcgccgc
     5521 accggcgcgc gaggtcttta tcttcctcat caatggcgcg aaagaacgcg cgcccgcggc
     5581 ggcgatggag ctcatccagc ggctatcctg atgccgcggc gcgacggccg cccggatgct
     5641 atcaaaatca tagctgaaag taggacccgg attacacctt gtttcgctct cggcggtcag
     5701 gccatgccgc gccaggcggg tcaaatcatg attgccaaaa caagaaggga gacaagagtc
     5761 atggcctatg tgttcgaaac cgtacccgca gatcggggcc tgatcgacct tgatgacccg
     5821 cgcgaagtca actggtggag caagcaattc ggttgcagcg aggagcagct caggcaagcg
     5881 gtggaaaagg tggggcgttc ggcggcgaag gtcgagcagt tgctcgacgg tcacgacatc
     5941 ctttcggttt gagtcggcgc gccgagcgtg gcgcggctcc aa
//
LOCUS       DASWIY010000072        12328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1524154, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000072 DASWIY010000000
VERSION     DASWIY010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 12328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12328
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524154"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2624
                     /gene="rpoB"
                     /locus_tag="VGA59_02750"
     CDS             <1..2624
                     /gene="rpoB"
                     /locus_tag="VGA59_02750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX9718622.1"
                     /translation="HDLINSKPISAALKEFFGASQLSQFMDQNNPLSEITHKRRVSAL
                     GPGGLTRERAGFEVRDVHVTHYGRVCPIETPEGPNIGLINSLALYARLNEYGFIETPY
                     RRVIDGKVTMQIDYLSAIEEGKYVIAQANAALDKEGRLTGDLVSAREAGESVLVGADR
                     IQYMDVSPAQIVSVAASLVPFLEHDDANRALMGANMQRQAVPVLRPEKAFVGTGIERV
                     SAIDSGTVVTATRGGVVDYVDATRIVVRVHDAEAQAGEVGVDIYNLIKYQRSNQNTNI
                     HQRPIVKRGDTIAKGDVIADGASTDLGELALGQNMLVAFMPWNGYNFEDSILISERVV
                     ADDRYTSIHIEELVVMARDTKLGAEEITRDIPNLSEQQLNRLDESGIIYVGAEVQPGD
                     TLVGKVTPKGETTLTPEEKLLRAIFGEKASDVKDTSLRVDQGSQGTVIDVQVFTREGI
                     QRDKRAQQIIDDELKRFRLDLNDQLRIVEADAFDRIEKLLNGRVANGGPQKLAKGTRI
                     DKAYLASVEKFHWFDIRPAEDEIASQLESIKNSIEQQRHSFDLAFEEKRKKLTQGDEL
                     PAGVLKMVKVYLAVKRRLQPGDKMAGRHGNKGVVSKIVPVEDMPYMADGTPCDIVLNP
                     LGVPSRMNVGQVLEVHLGWAAKGIGQRIGDMLQAEAKQAELRAFLDQLYNGSGRKEDV
                     VGLADEDVLEMAENLAKGVPFATPVFDGASEEEIRGMLKLAYPEEVAKLKGLTATRTQ
                     AMLYDGRSGDQFERPVTVGYMHVLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGG
                     QRFGEMEVWALEAYGAAYTLQEMLTVKSDDVQGRTKVYESIVKGEHAIEAGMPESFNV
                     LVKEIRSLGIDMELERS"
     gene            2645..6874
                     /gene="rpoC"
                     /locus_tag="VGA59_02755"
     CDS             2645..6874
                     /gene="rpoC"
                     /locus_tag="VGA59_02755"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX9718623.1"
                     /translation="MKSLLDLFKQFTPDEHFDAIRIGMASPEKIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIDLAAPCAHIWFLKSLPSRLGLVLDMTLRDIERVLYFEAYVVTDPGMTPLKKFSIM
                     SEDDYEAKRKEYGDEYVAKMGAEGIKDLLEGIDIEVEIEKLRGDLTGSEVKVKKNAKR
                     LKVLEAFKKSGIKPGWMVMEVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNSR
                     LRRLLELKAPEIIARNEKRMLQEAVDSLLDNGRRGKAMTGANKRALKSLADMIKGKSG
                     RFRQNLLGKRVDYSGRSVIVVGPTLKLHQCGLPKLMALELFKPFIFSRLEAMGIATTI
                     KAAKKEVESGTPVVWDILEEVIKEHPVMLNRAPTLHRLGIQAFEPILIEGKAIQLHPL
                     VCAAFNADFDGDQMAVHVPLSVEAQMEARTLMLASNNVLFPANGEPSIVPSQDVVLGL
                     YYTTRERINAKGEGLIFADTGEVQRALDAGEAELTAKVTVRLTEWNRDKDTNEFVPST
                     SLVDTTVGRALLSEILPKGLPFSNLNKALKKKEISKLINASFRKCGLKETVVFADKLL
                     QNGFRLATKAGISIAVDDMLVPPQKPEILSRAEKEVKEIEQQYVSGLVTAGERYNKVV
                     DIWGKAGDEVSKVMMAQLAKERVVDRHGKEVDQESFNSIYMMADSGARGSAAQIRQLA
                     GMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRR
                     LVDVTQDLVVTEVDCETRDGSLMRAIVEGGEVIESLRDRILGRVAADDVLHPETRETI
                     AAAGTMLDEDLIEELEGAGVDEVKVRTALTCATRFGICAKCYGRDLGRGGLVNVGEAV
                     GVIAAQSIGEPGTQLTMRTFHIGGAASRAAIASHVEAKSNGVIGFNATMRYVTNSKGE
                     LVVIARSGEIVIHDEHGRERERHKVPYGAILAVKADQQIKAGGVLASWDPLTRPIITE
                     FAGKVKFENVEEGLTVAKQVDEVTGLSTLVVIKFDPKRSAKVIRPQVKLIDAAGNEVK
                     IPGTDHSVTIGFPVGALIQVRDGQDVAGGEVLARIPIEGQKTRDITGGLPRVAELFEA
                     RSPKDKGVLAEITGTVSFGKETKGKIRMQITDPEGKVWEELVPKEKNILVHEGQVVNK
                     GESVVDGPADPQDILRLLGAEELARYIVDEVQDVYRLQGVKINDKHIEVIVRQMLRRV
                     VVDNVGDAAYIAGEQVERSEILDTCDALRAEGKIPPTWSNLLLGITKASLSTDSFISA
                     ASFQETTRVLTEAAIMGKRDELRGLKENVIVGRLIPAGTGMAYHQARKAKDQMDEAER
                     RAIAEAEAAELASATEGATAPEASSEGAATE"
     gene            6955..7533
                     /locus_tag="VGA59_02760"
     CDS             6955..7533
                     /locus_tag="VGA59_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX9718624.1"
                     /translation="MPSSVVLAAVAAVLLFWAVGAYNRLMRLRGEAKTAFASLEAELS
                     KQVALVGECLPAPEEATQPAALDGGGSFWAALHGAAAQFAASLAAARTRPLEPEGIAA
                     LSAAQDVLAMAWERAERDDAHDLAGARLPETLTARRAQLALQTHAAIDQFDRAVCRYN
                     EAIAQFPAVLLAWLFGFKPGRSVRPIATAARP"
     gene            7545..8933
                     /gene="rsmB"
                     /locus_tag="VGA59_02765"
     CDS             7545..8933
                     /gene="rsmB"
                     /locus_tag="VGA59_02765"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX9718625.1"
                     /translation="MAGAVKGHDAAARGGPASVPLWRLLQASADVVAAVRAGQSATAA
                     LQRVEGQLRAGAQALAFQALRYLGRAEALRALLARRAPPPQADALLCTALALCWRDAG
                     APYEVFTLVDQAVEAAKRNARTKGQANFINACLRRLLRERGALVAATDRDPVALWNHP
                     LWWIERLKADHPRHWQRILEANNSQAPMTLRVNARKSTPARYLAALAGAEIAAAPVGE
                     HGVMLAHPRPVHEVPGFDEGVVSVQDAAAQLAAPLLLQGMGAGRTAPLRILDACAAPG
                     GKTAHLLELADADVTALDVDPVRCERIHETLERLGLKARVIAADAGQPSAWRDGRPFD
                     AILLDAPCTASGIVRRHPDVRWLRRESDIAQLAAVQARLLAVLWPLLAAGGRLLYCTC
                     SVFRAEGELQAQTFLAHNTDAVLQDGPGHLMPQTGAKANGVPDNLEGDHDGFYYALFE
                     KRTVLESPAQGL"
     gene            8968..9519
                     /locus_tag="VGA59_02770"
     CDS             8968..9519
                     /locus_tag="VGA59_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEX9718626.1"
                     /translation="MTLASPALAQPPTTEIAQLRVVRSDEGILLSAHVTFELPAVIDD
                     ALDKGIPMFFVAEAALFRDRWYWYDKQVAIVARHMRLSYQPLTRRWRLVVSHTPIGSS
                     GLALGQTFDTREEAMAAVQRISHWKIADSADVDPDARYNVDFRFRLDLSQLPRPFQIG
                     ALGHADWNITAARNQRLGFEILK"
     gene            9558..11804
                     /locus_tag="VGA59_02775"
     CDS             9558..11804
                     /locus_tag="VGA59_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9718627.1"
                     /translation="MRSSAAFRWAVLVAAAVIVAIAIVLLFLLTQATGNRELYERNYG
                     RLFIVNVVVAALLLLVLVWVAWRLLSRVRRGKFGSRLLVKLAAIFALVGFLPGVLIYV
                     VSYQFVSRSIESWFDVKVEGALDAGLNLGRATLDTLANDLANKTRAAAVQLAETPDAS
                     AALALERLREQLPANDVILWSGTGQLIASAGQSRFQLNPERPTPQQLRNVRSQRSLAL
                     IEGLEEAGLHPVSHARIKALAVVSHPSLGLLAEPRILQVTQSLPPTLMANAIAVQEAN
                     REYQERALAREGLRRMYIGTLTLSLFLAVFGAVLLAVVLGNQLARPLLLLAAGVREVA
                     AGDLSPKPVLQGKDELGGLTRSFAEMTQQLADARAAVDKSMGQVSAARANLQTILDNL
                     TAGVIVLDAQGNIQSSNPGATRILRAPLAAYEGLPLASVEGLQAFGASVQQQFDEYMN
                     ERKQHGLDHWQQSFELNAAQPGSPQHAVTLVARGAEMPADQPGKAARLLVFDDISEIV
                     SAQRAQAWGEVARRLAHEIKNPLTPIQLSAERLEMKLAGKVGATEQAIVTKSVHTIVD
                     QVDAMKRLVNEFRDYARLPAAQLKPVDLNALVSDVLQLYAREGGERGDESARVPVRME
                     LDPDCPKIMGDAQQLRQVLHNLLQNAQDATEGAALREVTVKTQWNPQARRARLIVQDT
                     GAGFPDHILKRAFEPYVTTKSKGTGLGLAVVKKIADEHGARIDLKNRVEDGVVLGAQV
                     SLSFAVAA"
     gene            11844..>12328
                     /locus_tag="VGA59_02780"
     CDS             11844..>12328
                     /locus_tag="VGA59_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9718628.1"
                     /translation="MANILVVDDELGIRDLLSEILNDEGHSVELAENAAQARAARGRS
                     RPDLVLLDIWMPDTDGVTLLKEWSTTGMLSMPVIMMSGHATIETAVEATKIGAQSFLE
                     KPITMQKLLKAVENGLSRNGGPAPRKPGAGSLVAPIIELTVEAAMTGGQSLPAAVDMG
                     P"
BASE COUNT         2255 a         4094 c         4139 g         1840 t
ORIGIN      
        1 cgcacgacct gatcaactcc aagccgattt ccgcggcgct caaggagttc ttcggcgcga
       61 gccagctgtc gcagttcatg gaccagaaca acccgctgtc cgagatcacg cacaagcgcc
      121 gcgtctcggc ccttggcccg ggcggcctga cgcgcgagcg cgccggcttc gaagtgcgcg
      181 acgtgcacgt cacgcactac ggccgcgtgt gcccgatcga gacgccggaa ggtccgaaca
      241 tcggcctgat caactcgctg gccctgtacg cccgcctgaa cgaatacggc ttcatcgaga
      301 cgccgtaccg ccgggtgatc gacggcaagg tgacgatgca gatcgattac ctctcggcca
      361 tcgaggaagg caagtacgtg atcgcccagg ccaacgcggc gctggacaag gaaggccgcc
      421 tgaccggcga cctggtgtcc gctcgcgaag ccggcgaatc ggtgctggtc ggcgccgacc
      481 gcatccagta catggacgtg tcgcccgcgc agatcgtgtc ggtggccgcc tcgctggtgc
      541 cgttcctcga gcacgacgac gcgaaccgcg cgctgatggg cgccaacatg cagcgccagg
      601 cggtgcccgt gctgcgccct gaaaaggcat tcgtcggcac cggcatcgag cgcgtctccg
      661 cgatcgactc gggaaccgtc gtcaccgcga cccgcggcgg cgtggtcgat tacgtcgacg
      721 cgacccgcat cgtggtgcgc gtgcacgacg ccgaagccca ggccggcgaa gtcggcgtgg
      781 acatctacaa cctgatcaag taccagcgtt ccaaccagaa caccaacatc caccagcgcc
      841 cgatcgtcaa gcgcggcgac accatcgcca agggcgacgt gatcgccgat ggcgcatcga
      901 ccgacctggg cgaactggcc ctgggccaga acatgctggt ggcgttcatg ccctggaacg
      961 gctacaactt cgaggactcg atcctgattt ccgaacgtgt ggtggccgac gaccgctaca
     1021 cctcgatcca cattgaggaa ctggtggtga tggcccgcga caccaagctg ggtgccgagg
     1081 agatcacgcg cgacatcccg aacctgtcgg agcagcagct caaccgcctg gacgagtccg
     1141 gcatcatcta cgtcggcgcc gaagtgcagc ccggcgacac gctggtcggc aaagtgacgc
     1201 ccaagggcga aaccacgctc acgccggaag aaaaactgct gcgcgcgatc ttcggcgaga
     1261 aggcttccga cgtgaaggac acgtcgctgc gcgtggacca gggctcgcaa ggcaccgtga
     1321 tcgacgtgca ggtcttcacc cgtgaaggca tccagcgcga caagcgcgcc cagcagatca
     1381 tcgacgacga gctcaagcgc ttccgcctcg acttgaacga ccagctgcgc atcgtcgaag
     1441 ccgacgcgtt cgaccggatc gagaaactgc tgaacggccg ggtcgccaac ggcggcccgc
     1501 agaagctcgc caagggcacc aggatcgaca aggcttacct ggcgtcggtg gagaagttcc
     1561 actggttcga catccgtccg gccgaagacg aaatcgcctc gcaactcgag tcgatcaaga
     1621 actcgatcga gcagcagcgt cacagcttcg acctcgcctt cgaggaaaag cgaaagaagc
     1681 tgacgcaggg cgacgagttg ccggcgggcg tgctgaagat ggtcaaggtc tacctcgctg
     1741 tcaagcgccg cctgcagccg ggcgacaaga tggccggccg tcacggcaac aagggcgtgg
     1801 tttcaaagat cgttccggtc gaggacatgc cctacatggc cgacggcacg ccttgcgaca
     1861 tcgtgctcaa cccgctgggc gtgccctcgc gcatgaacgt gggccaggtg ctggaagtgc
     1921 acctgggctg ggccgccaag ggcatcggcc agcgcatcgg cgacatgctg caggccgagg
     1981 ccaagcaggc cgagctgcgc gcgttcctcg accagttgta caacggctcc ggccgcaagg
     2041 aagacgtggt ggggctggcc gatgaagacg tgctggagat ggcggagaac ctggccaagg
     2101 gcgtgccgtt cgccaccccg gtgttcgacg gcgcctcgga agaggaaatc cgcggcatgc
     2161 tcaagctcgc ctatccggaa gaggtcgcca agctcaaggg cctcaccgcg acccgtaccc
     2221 aggcgatgct gtacgacggc cgctcgggcg accagttcga gcgtccggtc accgtgggct
     2281 acatgcacgt gctcaagctc caccacctgg tggacgacaa gatgcatgca cgttcgaccg
     2341 gcccctacag cctggtcacg cagcagccgc tgggcggcaa ggcccagttc ggcgggcagc
     2401 gtttcggcga gatggaagtg tgggcgctgg aagcctacgg cgccgcctac acgctgcagg
     2461 aaatgctgac cgtcaagtcg gacgacgtac agggccgcac caaggtctac gaaagcatcg
     2521 tcaagggcga gcacgccatc gaagccggca tgccggaatc gttcaatgtc ctggtcaagg
     2581 aaatccgttc cttgggcatc gacatggaac tggaacgtag ctaatcgcag gaaggtcaca
     2641 ctttatgaaa tcattactcg acctgttcaa gcagttcacg ccggacgagc atttcgatgc
     2701 catccgcatc ggcatggcct cgcccgagaa gatccgttcc tggtctttcg gcgaagtcaa
     2761 gaagccggaa accatcaact accgcacctt caagcccgag cgcgacggcc tgttctgcgc
     2821 caagatcttc ggccccatca aggactacga gtgcctgtgc ggcaagtaca agcgactgaa
     2881 gcaccgcggc gtgatctgcg agaagtgcgg cgttgaagtc acccagacca aggtgcgccg
     2941 cgaacgcatg ggccacatcg acctggccgc accctgcgcc catatctggt tcctgaagtc
     3001 gctgccgtcg cgcctgggcc tggtgctgga catgacgctg cgggacatcg agcgcgtgct
     3061 gtatttcgaa gcctatgtgg tgaccgaccc cggcatgacc ccgctgaaga agttcagcat
     3121 catgtccgag gacgactacg aggccaagcg caaggaatac ggtgacgagt acgtcgccaa
     3181 gatgggcgcc gaaggcatca aagatctgct cgaaggcatc gatatcgaag tcgagatcga
     3241 aaagctgcgc ggcgacctga ccggttcgga agtcaaggtc aagaagaacg ccaaacggct
     3301 gaaggtgctg gaagccttca agaagtccgg catcaagccc ggctggatgg tgatggaagt
     3361 gctgcccgtg ctgccgccgg acctgcgtcc gctggtgccg ctggacggcg gccgcttcgc
     3421 gacctccgac ctgaacgacc tgtaccgccg cgtgatcaac cgcaacagcc gcctgcgccg
     3481 cctgctggag ctgaaggctc ccgagatcat cgcacgcaac gaaaagcgga tgctgcagga
     3541 agccgtggac agcctgctgg acaacggccg ccgcggcaag gcgatgacgg gcgccaacaa
     3601 gcgcgccctg aagtcgctgg ccgacatgat caagggcaag agcgggcgct tccgccagaa
     3661 cctgctgggc aagcgcgtcg actactcggg ccgctcggtc atcgtggtcg gcccgacgct
     3721 caagctgcac caatgcggcc tgcccaagct gatggcgctc gagctgttca aacccttcat
     3781 cttctcgcgc ctggaagcca tgggcatcgc caccaccatc aaggcggcga agaaggaagt
     3841 ggaatcgggc acgccggtgg tgtgggacat cctcgaagag gtgatcaagg aacacccggt
     3901 gatgctgaac cgcgccccga cgctgcaccg cctgggcatc caggccttcg agccgatcct
     3961 gatcgaaggc aaggcgatcc agttgcaccc gctggtctgc gccgccttca acgccgactt
     4021 cgacggcgac cagatggccg tgcacgtgcc gctgtcggtg gaagcgcaga tggaagcccg
     4081 caccctgatg ctggcctcca acaacgtgct gttccccgcc aacggcgaac cgtccatcgt
     4141 gccgtcgcag gacgtggtgc tgggcctgta ctacacgacg cgcgagcgca tcaacgccaa
     4201 gggcgagggc ctgatcttcg ccgacaccgg tgaagtgcaa cgcgcgctgg acgcgggcga
     4261 ggccgagctc accgccaagg tcacggtgcg cctcacggag tggaaccggg acaaggatac
     4321 caacgaattc gtgccgtcca cttcgctggt ggacaccacc gtcggacgcg cgctgctgtc
     4381 ggaaatcctg cccaagggcc tgccgttctc caacctgaac aaggcgctca agaagaagga
     4441 aatctccaag ctgatcaacg cgtcgttccg caagtgcggc ctgaaggaaa ccgtggtgtt
     4501 cgccgacaag ctgctgcaaa acggcttccg gctggcgacc aaggccggca tctcgatcgc
     4561 ggtggacgac atgctggtgc cgccgcaaaa gcccgagatc ctgagccgcg ccgagaagga
     4621 agtcaaggaa atcgagcagc aatacgtctc gggcctggtc acggccggcg agcgctacaa
     4681 caaggtggtg gacatctggg gcaaggccgg cgacgaagtc tccaaggtca tgatggccca
     4741 gctggccaag gaacgggtgg tggaccgcca cggcaaggaa gtggaccagg agtccttcaa
     4801 ctccatctac atgatggccg actccggcgc ccgcggctcg gccgcccaga tccgccagct
     4861 ggcgggcatg cgcggcctga tggccaagcc ggacggctcg atcatcgaga cgccgatcac
     4921 ggccaacttc cgcgaaggcc tgaacgtgct gcagtacttc atctccaccc acggcgcccg
     4981 caagggcctg gcggatacgg cgttgaagac cgcgaactcg ggctacctga cgcggcgcct
     5041 ggtcgacgtg acccaggacc tggtcgtcac cgaagtggac tgcgaaaccc gcgatggctc
     5101 gctgatgcgc gcgatcgtgg agggcggtga agtgatcgag tcgctgcgcg atcggatcct
     5161 gggccgcgtc gcggccgatg acgtgctgca cccggaaacg cgtgaaacca tcgccgccgc
     5221 cggcaccatg ctggacgagg acctgatcga ggagctcgaa ggcgcgggcg tcgatgaagt
     5281 gaaggtccgc acggcgctga cctgcgcgac gcgattcggc atctgcgcca agtgctacgg
     5341 ccgcgacctc ggccgcggcg gcctggtcaa tgtcggcgag gcggtcggcg tgatcgccgc
     5401 ccagtcgatc ggcgagcccg gaacgcagct gacgatgcgc accttccaca tcggtggtgc
     5461 cgcctcgcgc gccgcgatcg cctcccacgt cgaagcgaag tccaacggcg tgatcggctt
     5521 caacgcgacg atgcgctacg tcaccaacag caagggcgag ctggtcgtga ttgcccgctc
     5581 cggcgagatc gtcatccacg acgagcacgg aagagaacgc gagcgccaca aggtgccgta
     5641 cggcgcgatc ctggccgtga aggccgacca gcagatcaag gccggcggcg tgctcgccag
     5701 ctgggatccg ctgacgcgcc cgatcatcac ggaattcgcc ggcaaggtga agttcgagaa
     5761 cgtcgaggaa ggcctcaccg tcgccaagca ggtcgatgaa gtcaccggcc tgtcgacgct
     5821 ggtggtgatc aagttcgacc ccaagcgctc ggccaaggtg atccgcccgc aggtcaagct
     5881 gatcgacgcc gccggcaacg aggtgaagat ccccggcacc gaccactcgg tgacgatcgg
     5941 tttcccggtc ggcgccctga tccaggtgcg cgacggccag gacgtggccg gcggcgaagt
     6001 gctggcccgc atcccgatcg aaggccagaa gacgcgcgac atcacgggcg gcttgccgcg
     6061 cgtggccgag ctgttcgaag cgcgttcgcc caaggacaag ggcgtgctgg ccgagatcac
     6121 cggcaccgtg tcgttcggca aggaaaccaa gggcaagata cgcatgcaga tcacggaccc
     6181 ggagggcaag gtctgggagg agctggtgcc caaggaaaag aacatcctgg tgcacgaggg
     6241 ccaggttgtg aacaagggcg aatccgtggt ggacggcccg gccgatccgc aggacatcct
     6301 gcgcctgctg ggcgccgagg aactggcgcg ctacatcgtc gacgaggtgc aggacgtgta
     6361 ccgcctgcag ggcgtgaaga tcaacgacaa gcacatcgag gtgatcgttc gccagatgct
     6421 gcgccgggtc gtggtcgaca acgtcggtga cgctgcctac atcgcgggcg aacaggtgga
     6481 gcgttcggaa atcctggata cctgcgacgc attgcgcgcc gaaggcaaga tcccgccgac
     6541 ctggagcaac ctgctgctgg gcatcaccaa ggcctcgttg tcaaccgact cgttcatctc
     6601 cgcggcttcc ttccaggaaa ccacgcgggt gctgaccgaa gcggcgatca tgggcaagcg
     6661 cgacgagctg cgtggcctga aggagaacgt catcgtcggg cggctcatcc ccgcgggcac
     6721 cggcatggcc taccaccagg cacgcaaggc caaggaccag atggacgagg ccgagcgccg
     6781 cgccattgcc gaggccgagg cggcggaact cgccagcgcg accgaaggcg ccaccgcgcc
     6841 agaggccagc agcgaaggcg cggccaccga gtaaaccggc ctttcaccgt aaactagcgc
     6901 cccagcccat tcgcgggttg gggcgctttt ttttgttggg ggttgccgcc ggctatgccg
     6961 tcttccgtcg ttctcgcagc cgtcgccgcg gtcctgctgt tctgggccgt gggcgcctac
     7021 aaccgcctga tgcggcttcg cggcgaagcc aagacggcct tcgccagcct ggaggccgag
     7081 ctgtccaagc aggtcgcgct ggtgggcgag tgcctgccgg caccggagga ggccacccag
     7141 cccgccgcgc tcgatggagg gggctctttc tgggccgcac tgcatggcgc ggcggcgcaa
     7201 ttcgcggcgt cgctggccgc ggcgcgcacg cgcccgctgg agccggaggg aatcgccgcc
     7261 ctgagcgccg cgcaggacgt cctggcgatg gcctgggaac gggccgagcg tgacgacgcc
     7321 catgacctgg ccggagcgcg actgcccgaa accctgaccg cgcggcgtgc ccagctggcg
     7381 ctgcagacgc acgcggcaat cgaccagttc gaccgcgccg tgtgccgcta caacgaagcc
     7441 atcgcgcaat tcccggctgt gctgctggca tggctgttcg gcttcaagcc cggacgcagc
     7501 gtgcggccca tcgccaccgc cgcccggcca taagcggcca cgcgatggcc ggcgcggtca
     7561 agggtcatga cgccgcggcg cgtggcggtc ccgcctcggt cccgctgtgg cgattgctcc
     7621 aggcgagcgc ggacgtcgtg gccgcggtgc gcgccggcca atcggccacc gccgcgctcc
     7681 agagggtcga agggcagctg cgggccggcg cgcaagccct ggcattccag gccctgcgct
     7741 acctgggccg tgccgaagcc ctgcgcgcat tgctcgcgcg ccgcgcgccg ccgccgcagg
     7801 ccgatgcgct gctgtgtacc gcactggcct tgtgctggcg cgacgccggc gcaccgtacg
     7861 aggtcttcac gctggtggat caggccgtcg aggccgccaa gcgaaatgcc aggaccaagg
     7921 gccaggccaa tttcatcaat gcgtgcctgc gccgtttgtt gcgggagcgc ggcgcgctgg
     7981 tcgcagcgac cgaccgcgat ccggtggcgc tgtggaacca cccgctctgg tggatcgagc
     8041 gattgaaagc ggaccacccg cggcactggc agcgcattct agaagccaac aacagccagg
     8101 cgccgatgac attgcgcgtc aacgcgagga aatccacgcc ggcccggtac ctggcggcgc
     8161 tggccggcgc ggaaatcgcc gccgcgccgg tgggcgagca tggcgtgatg ctcgcccacc
     8221 cgcgacctgt gcacgaagtg ccgggcttcg acgaaggcgt ggtatcggtg caggacgcgg
     8281 ctgcccaatt ggcggcaccg ctgttgctgc aaggcatggg cgcgggacgg acggcgccgc
     8341 tgcgcatcct cgacgcctgc gccgcgcccg gcggcaagac agcgcacctg ctggaactcg
     8401 ccgatgccga cgtcaccgcg ctggacgtgg atccggtccg ttgcgagcgc atccatgaga
     8461 ccctggagcg cctcggcttg aaagcccgcg tgatcgccgc cgacgccggc cagccttccg
     8521 catggcggga cggcagaccg ttcgacgcga tcctgctcga cgcgccctgc accgcctcgg
     8581 ggatcgtgcg tcgccacccc gatgtgcggt ggctgcgccg tgaaagcgac atcgcccagc
     8641 tggccgcggt gcaggcgcgc ctgctcgccg tgctgtggcc gctgctggcg gcgggcgggc
     8701 gcctgctcta ctgcacctgt tcggtgtttc gcgctgaggg cgagctgcag gcgcaaacgt
     8761 ttcttgcgca caacacagac gccgttttgc aggatgggcc gggccattta atgccccaaa
     8821 cgggagcaaa ggccaatggc gtcccggaca atctggaagg tgatcacgac ggtttctatt
     8881 acgcgctgtt tgaaaaacgc acagtgctgg agtctcctgc gcagggcctt tgaccgcgct
     8941 gcctggggcg ttctggcctg ttgcgtgatg acgctcgcct cacctgcatt ggctcagccg
     9001 cccactaccg aaatcgcgca gctgcgggtg gtgcgcagcg acgaaggtat cctgctgtcg
     9061 gcgcatgtca ctttcgagct gccggcggtg atagacgatg cgctcgacaa gggcattccg
     9121 atgtttttcg tggccgaagc cgcgctgttc cgcgaccgct ggtactggta cgacaagcag
     9181 gtcgccatcg tcgcgcgcca tatgcgcctg tcgtaccagc cgctgacgcg ccgctggcgc
     9241 ctggttgtgt cccacacccc catcggcagc tcgggcctgg cgctggggca gaccttcgac
     9301 acgcgcgagg aggcgatggc cgcggtgcag cgcatttcgc actggaagat cgccgattcc
     9361 gccgacgtgg accccgacgc gcgctataac gtcgacttcc gcttccggct cgacttgtcg
     9421 cagctgccgc ggccgttcca gatcggcgcg ctcggccatg ccgactggaa catcacggcc
     9481 gcccgcaacc agcgcctggg cttcgagatc ctgaagtgag cacccagcct tcccccgaga
     9541 catcgtcccg gtacccgatg cgcagttcgg ccgcattccg ctgggcggtg ctcgtggccg
     9601 cagcggtcat cgtggccatc gccatcgtcc tgctgttcct gctgacccag gcgacgggta
     9661 accgcgagct gtacgagcgc aactacgggc ggctgttcat cgtcaacgtc gtggtggccg
     9721 ccctgctgct gctggtcctc gtctgggtgg cctggcggct gctgtcgcgc gtgcgccgcg
     9781 gcaagttcgg cagccgtctg ctggtcaagc tggccgcgat ttttgcgctg gtcggcttcc
     9841 tgccgggcgt gctgatctac gtcgtgtcct accagttcgt ctcgcgttcc atcgaaagct
     9901 ggttcgacgt gaaagtggag ggcgcgctcg acgcgggact gaacctcggc cgcgccacgc
     9961 tggacacctt ggccaacgac ctggccaaca agacccgggc cgcggcggtg caacttgccg
    10021 agacgcccga cgcgtcggcc gcgctggcgc tggagcggct gcgcgagcag ctgcccgcca
    10081 acgacgtcat cctgtggagc ggcacggggc agctgatcgc cagcgccggc cagtcgcggt
    10141 tccagctcaa tcccgaacgg cccacgccgc agcagctgcg caacgtgcgg tcacagcgct
    10201 cgctggcctt gatcgagggg ctggaggagg ccggcctgca cccggtgagc cacgcgcgca
    10261 tcaaggcgct ggccgtggta tcccatccca gtcttggcct tctggccgag ccgcgcatcc
    10321 tccaggtgac ccagagcctg ccgcccacgc tgatggccaa tgcgatcgcg gtgcaggagg
    10381 ccaaccgcga gtaccaggag cgcgcgctgg cccgcgaagg cctgcggcgg atgtacatag
    10441 gcaccttgac gctgagtctt ttcctggcgg tgttcggcgc cgtgctgctg gccgtggtgc
    10501 tggggaacca gctcgcgcgg ccgctgctgt tgctggccgc gggtgtgcgc gaggtggccg
    10561 ccggcgatct ctcgcccaaa cctgtccttc agggcaagga cgagctcggc ggcctgacgc
    10621 ggtctttcgc ggagatgacg cagcagctgg cggacgcgcg cgccgccgtc gacaagagca
    10681 tgggccaggt cagcgccgcc cgcgcgaacc tgcagaccat tctggacaac ctcaccgccg
    10741 gcgtgatcgt gctggacgcg cagggcaata tccagtcgtc caacccgggc gcaacgcgca
    10801 tcctgcgcgc accgctggcg gcgtacgagg gcctgccgct ggcgagcgtc gaagggctcc
    10861 aggccttcgg cgcttcggtg cagcagcagt tcgacgagta catgaacgag cgcaagcagc
    10921 acggcctgga ccactggcag caatcgttcg agctcaacgc cgcccagccc ggctcgccgc
    10981 agcacgccgt cacgctggtc gcccgcggcg cggaaatgcc ggcggaccag ccgggcaagg
    11041 cggcacggct gctggtgttc gacgacatct cggagatcgt ttcggcgcaa cgcgcccagg
    11101 cctggggcga ggtggcgcgc cggctggccc acgagatcaa gaacccgttg acgccgatcc
    11161 agttgtcggc cgagcggctg gagatgaagc tggccggcaa agtgggcgcg accgagcagg
    11221 cgatcgtcac aaaatcggtg cacaccatcg tggaccaggt cgacgccatg aagcggttgg
    11281 tcaacgaatt ccgcgactat gcgcggctgc ccgcggcgca gctcaagccg gtggacctca
    11341 atgcgctggt aagcgacgtg ctgcagctgt atgcgcgcga gggcggcgag cgcggcgacg
    11401 aatcggcgcg tgtgccggtg cgcatggagc tcgatccgga ttgcccgaag atcatgggcg
    11461 acgcgcagca gctgcgccag gtgctgcata acctgctgca aaacgcccag gacgccacgg
    11521 aaggcgcggc gctgcgcgaa gtgacggtga agacccagtg gaacccgcag gcccggcgtg
    11581 cccgcctgat cgtgcaggac accggcgccg gttttccgga tcacatcctc aagcgcgcct
    11641 tcgagcccta tgtcaccacc aagtccaagg gcaccgggct gggcctggcc gtggtcaaga
    11701 aaatcgccga cgaacacggc gcgcggatcg acctgaaaaa ccgggttgag gacggtgtcg
    11761 tcctgggcgc ccaagtatcg ctatcattcg cagttgcggc ttagcaacac ccactcccgc
    11821 cgcagggggt agcgctggaa aacatggcaa acattctcgt ggtagacgac gagctgggca
    11881 tccgggacct gctctcggaa atcctcaacg acgagggcca cagcgtcgag ctcgcggaaa
    11941 acgcggctca ggcgcgcgcc gcgcgcggcc gctcccggcc cgacctggtg ctgctggaca
    12001 tctggatgcc cgacaccgat ggggtgacct tgctcaagga gtggtccacc acgggcatgc
    12061 tcagcatgcc ggtcatcatg atgagcgggc atgccaccat cgaaaccgct gtggaagcca
    12121 ccaagatcgg cgcgcaatcg ttcctggaaa agccgatcac catgcagaag ctgctgaagg
    12181 ccgtggaaaa cggcctttcg cgcaacgggg ggccggcgcc gcgcaagccc ggcgcgggta
    12241 gcctggtcgc gcccatcatc gaactcaccg tggaggcggc aatgaccggc gggcagagcc
    12301 tgcccgcggc cgtggacatg ggcccgca
//
LOCUS       DASWIY010000073         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_47002, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000073 DASWIY010000000
VERSION     DASWIY010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47002"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VGA59_02785"
     CDS             complement(<1..241)
                     /locus_tag="VGA59_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEX9718629.1"
                     /translation="MATPDQLLQALAAVIDPNTGKDFVSTKSVKNLQVHEGEVSFDVE
                     VGYPAKSQIPELRKALIAAAKGVPGVNNVSANVTSR"
     gene            392..>2380
                     /gene="metG"
                     /locus_tag="VGA59_02790"
     CDS             392..>2380
                     /gene="metG"
                     /locus_tag="VGA59_02790"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX9718630.1"
                     /translation="MPPQRKLFVTTALPYANGKFHIGHIMEYIQADIWVRFQRMQGHM
                     VHFVGADDAHGAPIMIAAEKAGKTPQAFVAEIAAGRREYLDGFHIQFDNWHSTESAEN
                     TELAQDIYRRLKANGLIAARTIEQFYDPVKGMFLPDRYIKGECPSCHSKDQYGDSCEV
                     CSSVYSPTDLIEPYSTLTGARPELRSSEHFFFKLSDPKVVDFLKGWTQDGKLQPEMAR
                     KASEWFEAGMNDWDISRDAPYFGIEIPDAPGKYFYVWLDAPIGYLASLKNHFDRGQAR
                     NHWHPASRTDANFDDFMADPAVEQVHFIGKDIVYFHTLFWPAMLEFSGRKTPSQVNVH
                     GFITVSGEKMSKSRGTGIDPLKYLSLGMNPEWLRYYIAAKLNGKVEDVDFNPDDFVAR
                     VNADLIGKYVNIASRAVKFLEGGKLVQSHVERLEPARLVDTVKDLYEAREYGKALREI
                     MAAADAVNHYFDGAAPWKLAKEGGRVEAAEVCSQCIEMFKVLTACLKPVLPELALHAE
                     AFLKCLPLDWQNAALPLGEGHQVGEYKHLMQRVDTKQLDALFEPPSEEPSPQPSPASG
                     RGSVPGGEELAPAIGIEDFAKVDLRIAKIVDCESVEGSNKLLRLTLDVGEGRHRNVFA
                     GIASAYQPSELIGKMTVMVANLAPRKMKFGISEGMVLAA"
BASE COUNT          465 a          758 c          756 g          401 t
ORIGIN      
        1 tccggctggt gacgttggcg gacacgttgt tgacgcccgg cactcccttg gcggccgcga
       61 tcagcgcctt gcgcagttcc gggatctggc tcttggccgg atagccgacc tcgacgtcga
      121 atgaaacctc gccctcgtgg acctgcaggt tcttgaccga cttggtgctg acgaagtcct
      181 tgccggtatt gggatcgatg acggccgcga gggcctggag taattggtct ggggtagcca
      241 tgggtagtgg attttcctgt tcgtcatgca gtgtaacgag tcggtcctcc gcgctatccc
      301 gacaaccgat ggggtcttgc cccgcgacgc cccgactgca gggcttggta cagcgcagct
      361 aaaattactg gttcccctgg aaaaggccct catgccccca cagcgcaagc tcttcgtcac
      421 caccgccttg ccgtacgcca acggcaagtt ccacatcggc cacatcatgg aatacatcca
      481 ggccgacatc tgggtgcggt tccagcgaat gcagggacac atggtgcatt tcgtcggagc
      541 ggacgatgca catggcgcgc ccatcatgat cgcggccgaa aaggccggca agacacccca
      601 ggccttcgtg gccgagatcg cggccgggcg ccgggaatac ctggacggct tccacatcca
      661 gttcgacaac tggcactcga ccgaaagcgc ggagaacacc gagctggcgc aggacatcta
      721 ccggcgcctg aaggccaacg gcctgatcgc ggcacgcacc atcgagcagt tctacgaccc
      781 cgtcaagggc atgttcctgc ccgaccgcta catcaagggc gagtgcccga gctgccactc
      841 caaggaccag tacggagact cctgcgaggt gtgcagcagc gtctattcgc ccaccgacct
      901 gatcgagccc tactcgacgc ttaccggtgc gcggcccgag ctgcgcagct cggaacattt
      961 cttcttcaag ctgtccgacc ccaaggtcgt cgactttctc aaaggctgga cgcaagacgg
     1021 caaactgcag cccgagatgg cgcgcaaggc cagcgaatgg ttcgaagcgg gcatgaacga
     1081 ctgggacatc agccgcgacg cgccttactt cggcatcgag attccggatg cgccgggcaa
     1141 gtatttctac gtctggctgg acgcgcccat cggttacctg gccagcctga agaaccactt
     1201 cgaccggggc caggcccgga accactggca ccccgcctcg cgcaccgacg ccaacttcga
     1261 tgacttcatg gccgacccgg cggtggagca ggtgcacttc atcggcaagg acatcgtcta
     1321 tttccacacg ctgttctggc ccgcgatgct ggagttctcc ggccgcaaga cgcccagcca
     1381 ggtgaacgtg cacggcttca tcaccgtgag cggcgagaag atgagcaaga gccgcggcac
     1441 cggcatcgat ccgctgaaat acctgtcgct gggcatgaac ccggagtggc tgcgctatta
     1501 catcgcagcc aagctcaacg gcaaggtgga ggacgtggac ttcaaccccg atgatttcgt
     1561 cgcgcgtgtc aatgcggacc tgatcggaaa gtacgtcaac atcgccagcc gcgccgtcaa
     1621 gttcctcgaa ggcggcaagc tggtgcaaag ccacgtggag cggctcgagc ccgcgcggct
     1681 ggtggacacg gtgaaggacc tgtacgaagc ccgcgaatac ggcaaggcgc tgcgcgagat
     1741 catggcggcc gcggacgcgg tgaaccatta tttcgacggc gcggcaccct ggaagctggc
     1801 caaggaaggc ggacgggtcg aggcggccga agtctgctcc caatgcatcg agatgttcaa
     1861 ggtgctcacc gcctgcctga agccggtgct gcccgagctc gcgttgcatg ccgaggcttt
     1921 cctcaagtgc ctgccgctcg actggcagaa tgcggcgctg ccgctgggcg agggccacca
     1981 ggtcggtgaa tacaagcacc tgatgcagcg ggtggatacg aagcagctcg acgcgctgtt
     2041 cgaaccgccg tcggaagagc cctcacccca gccctctccc gcgagcggga gagggagtgt
     2101 tcccggcggc gaagagctcg cgccggccat cggcatcgag gatttcgcga aggtcgacct
     2161 gcgcatcgcg aagatcgtcg actgcgagag cgtggaaggg tcgaacaagc tgctgcgcct
     2221 gacgctcgac gtgggcgaag ggcgccaccg caacgtgttc gccggcatcg cctcggcgta
     2281 tcagccctcg gagttgatcg gcaagatgac ggtgatggtc gcgaatctcg cgccgcgcaa
     2341 gatgaagttc ggcatcagcg aaggcatggt gctggccgcc
//
LOCUS       DASWIY010000074        12793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1524824, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000074 DASWIY010000000
VERSION     DASWIY010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 12793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12793
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524824"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VGA59_02795"
     CDS             <1..679
                     /locus_tag="VGA59_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9718631.1"
                     /translation="LPPEFAGRADDYVAEVLRMLPVLHGEGLVDAVDVFCERIAFSVA
                     QTEAVFAAARELGLPVKLHAEQLSDSGGAQLAARYGALSCDHLEWLSEAGAAAMARSG
                     SVAVLLPGAFYFLRDTRVPPVELLRRHGVPMAVATDCNPGSSPCTSPLLMLNMACTLF
                     RLTPQEALAGVTRNAARALGLRDRGMLAAGLRADFVLWDAQRPAQLSYTMGFNPRQQT
                     VFGGRSL"
     gene            676..1476
                     /gene="hutG"
                     /locus_tag="VGA59_02800"
     CDS             676..1476
                     /gene="hutG"
                     /locus_tag="VGA59_02800"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HEX9718632.1"
                     /translation="MTFILNRGTAPLLVSMPHTGTDIPQALREDFVPRALAVEDTDWH
                     LARVYNCLPALGASVLRPRYSRYVIDLNRPPDDMPMYPGASNTELCPTRFFTGEPLYP
                     DGRDPSAQERQRRLDTYWRPYHQALREELDRIRAEHGFALLWDAHSIRSEIPWLFDGV
                     LPDLNIGTAGGASAHASITQAVADAAARYADVSHIVNGRFKGGYITRHYGDPASHVHA
                     VQLEMCQNLYMKEKPPYAYQEALAAGIQPVLADMMAAALAACGGLYGR"
     gene            1466..2827
                     /locus_tag="VGA59_02805"
     CDS             1466..2827
                     /locus_tag="VGA59_02805"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEX9718633.1"
                     /translation="MGAEQQLYLAPAAWVNGAWAQDVLLTAGADGQWSSIAPNAPADA
                     QRGARRLDGPVVPGLVNGHSHAFQRAMAGLTEHSGAAEDDFWSWRDRMYSAANRITPD
                     QLEAIAAFLYCELLRSGYTHVCEFHYLHNGPEGRPYADPREMSLCLMRAAQRTGIGLT
                     LLPTLYMRSGFGAKTLREDQCRFASTPDSVLRIAELVARQGGRVAAGVAVHSLRAVDE
                     VALRELAAVATGMPVHIHIAEQRREVDDCIEHHGLRPVEWLLAKVALDASWNLVHSTH
                     CTQAELEGLRNRRASIVLCPSTEANLGDGIFDLPAWMGQSGSWAIGSDSHVTRSWQEE
                     LRLLEYSQRLGLRQRNVAARAALCESSAAALFEGALEGGSAAAGRPLGGLMAGQRADF
                     LVVDATSSALAGIPPDHLLDALVFSSPAPAFSEVFVAGQAVPAADPAVRAGFVRAMKE
                     LWT"
     gene            2997..3350
                     /locus_tag="VGA59_02810"
     CDS             2997..3350
                     /locus_tag="VGA59_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9718634.1"
                     /translation="MKREDMVETAAIDLDVMRQGAQGACALMKVLANPDRLLLLCQIA
                     DGERNVGELEEALGIGQPTLSQQLTVLRSESLVATRRDGKNIYYRLASPRAMAIMQAL
                     YAQFCASAKPKRSRQ"
     gene            3347..3778
                     /locus_tag="VGA59_02815"
     CDS             3347..3778
                     /locus_tag="VGA59_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX9718635.1"
                     /translation="MTIDWNHFTPWASLAGGLLIGLASTLFVLLNGRVLGISGILGGL
                     VKPRTGDALWRIAFIAGLLAAPTLWLAFAQPVAPRIEATGAMLAAAGLLVGWGTRYGS
                     GCTSGHGVCGLSRLSRRSLAATVTFMAAGFATVFLVRHVFA"
     gene            3789..4223
                     /locus_tag="VGA59_02820"
     CDS             3789..4223
                     /locus_tag="VGA59_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEX9718636.1"
                     /translation="MNRLIEFIAGLVFGIGLILSGMTDPGKVIGFLDLAGTWDPSLAL
                     VMAGAISVGVFGFGLARKRSTTLLGGPMQLPNTDVIDSRLLIGSAVFGAGWGLAGFCP
                     GPAVVATGAGHWQALVFTLAMLAGMAVHELSARPAPPALVRE"
     gene            4230..4361
                     /locus_tag="VGA59_02825"
     CDS             4230..4361
                     /locus_tag="VGA59_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718637.1"
                     /translation="MPPALLAPGLGVLWSGLAGPRLQQGFALLSMGVACALLARIAV"
     gene            4452..5684
                     /locus_tag="VGA59_02830"
     CDS             4452..5684
                     /locus_tag="VGA59_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9718638.1"
                     /translation="MSTMTASAPPATSLRQDAGVIGLVGLAHMISHYGQLLLAPLFPW
                     LKDAFGASYTELGFLMTLFFVVSCSVQAVSGFLVDRYGPRPILFGGLALLATAAFGYA
                     LSRGYWTMAIFSVLAGVGNGVFHPVNYTLLNRNVSPPRLGHAYSFHGITGSLGWALAP
                     AMLVPLAIVFSWRTALMAAGALALAVLGVMWLYRARLALPASAPRHAAAAHAEGTFEF
                     LRIPAVWMCFAFFFFYAVVLSVIQAFAPEAARQLHGVPVALAAMCLTVYMVCSAGGMV
                     RGGFLASDPARCERVVGMGFGVAALVALVIGVGTVPALAVPALFGAMGFASGIAGPSR
                     DLLVKRSTPENATGRVYGIVYSGLDIGQAISPLIFGALMDHQQYRGVWLGLVFMQAVL
                     ITSAFNVRRVRRTALTTA"
     gene            5719..7347
                     /locus_tag="VGA59_02835"
     CDS             5719..7347
                     /locus_tag="VGA59_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9718639.1"
                     /translation="MYASYFGLKYPPFSIAPDPRYLFMSERHREALAHLLYGLDGGGG
                     FVLLSGEIGTGKTTVCRLFLEQVPANCNVAYIFNPKLTAAELLQSVCDEFHIPVRQKD
                     GLPPTVKDYLDPLNAFLLQAHAAGRNNVLIIDEAQNLSAEVLEQLRLLTNLETSERKL
                     LQIILIGQPELRSMLARPELEQLAQRVIARYHLDALTPAETAQYVQHRCEVAGLSRPL
                     PFERKALRRVHQLARGVPRRINLLCDRALLGAFANSQAGVDRATIDKAAAEVFDTPAR
                     APVAWRRAAAILGLGLAAGAGLFATANHLTQDAALPPLAIQPAPAPAEEQAVAAPVAE
                     VDARQSPATAAPPPALLHDRDEAWRELALSWNAEAGEGEPCKALQKDQLHCFSKNLNL
                     ALIRELGRPGVLTLDAGTGAPTYAVLTALTRDSATLRAGGTEQTVTLAALAARWQGDF
                     ATLWRVPPGYTARIGSGLSGETLEWIAAGLAAVDGSTVPSGRRAPDAAVLKTQLRSFQ
                     LAQGMVPDGQVGPMTFMQLNRVAGVEEPRLRTEP"
     gene            7514..8377
                     /locus_tag="VGA59_02840"
     CDS             7514..8377
                     /locus_tag="VGA59_02840"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HEX9718640.1"
                     /translation="MSYILDALRKADAQRERDPARGIHAQPVRASIGERRSRDANRIG
                     FWAATAAVLAALASVGGYLYRDSGAPPAPTEVAVAAPPVTIVPPAAPPAAPPAAEPAA
                     AVLPPPVVVAPPPVVRAPEATAAPPVAARRPITKQVQGVPFEPTVARKGSRHSPAGAP
                     VATRAPPAPVPAQPVPDPAPSVATVAPRVAAVVSPAATVAPRVASVAPPAVAPSPVAE
                     PVAGGLPADAPKLAISGGVYSANVAQRMLIVNGQVFNEGSEVGAGVVLEEIRPKAAVL
                     RFRGSRYTVAY"
     gene            complement(8414..9121)
                     /locus_tag="VGA59_02845"
     CDS             complement(8414..9121)
                     /locus_tag="VGA59_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718641.1"
                     /translation="MNTSSRLLRVFRFCLLLAVAALESLAAAAPGVEPLDTSAANLLV
                     VLRESRGENKQDESLEPFGTLRATLGDGRQIELETSWYQYLGDMHIRLVFDGGQTMQS
                     ASPDDLARLSLSPQDALELAIANLRRVYGAPAVQPWSGNLMQVHGDAADLNSSYFLDR
                     EFWRALQYRHPEGLVVAVPRRGGLVYAPASDEAAVTNLRFSAAALYAGGPGARLSSAL
                     YLFKDGRWSVFQPPQAH"
     gene            9344..9901
                     /locus_tag="VGA59_02850"
     CDS             9344..9901
                     /locus_tag="VGA59_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX9718642.1"
                     /translation="MALQSPFFNKREPDAYSQRNNPNPNSAPATTTPAQQPANAPIAA
                     TAAANSAPASTGQEGESKLTVGPNIKLKGVEITDCDTLVVEGSVEATMDSRVIQISER
                     GSFKGSADIDIAEIRGQFDGNLTARQKLVIYATGKVTGRVRYGKVVIEEGGQLSGEVE
                     FGTATSRSASAGGPRPGLQVATAKV"
     gene            complement(9929..10831)
                     /locus_tag="VGA59_02855"
     CDS             complement(9929..10831)
                     /locus_tag="VGA59_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX9718643.1"
                     /translation="MRDFSQDHRWLSINTATVRKSRGVDLPLPRIIEACARRGIRAIS
                     PWRDQVAAAGLDTISRLVRTHGMELSGYCRGGMFPAVDAQGLRAARDDNRRAVDEACE
                     LRAACLVLVVGGLPGALAGMPAHKDIALARSQVRDGIGELLDYARSMHMPLAIEPLHP
                     MYAAERACINTLEQALDLCDELDPGPSGGEPRTATAALGVAADVYHVWWDPKLKAQIE
                     RAGKRRLLAFHVCDWLTPTTDLLNDRGMMGDGVIDIPRIRGWLEGQGFGGYSEVEIFS
                     SNNWWRRDHGEVLDTCIERHRRAV"
     gene            complement(10908..12095)
                     /locus_tag="VGA59_02860"
     CDS             complement(10908..12095)
                     /locus_tag="VGA59_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9718644.1"
                     /translation="MALSLQLPSTGGDLFQYTLRGAEPVKPAAGVRFNRIAYSAAHVV
                     ADPRAAIDPWLQCSIDWDATIAYRRRLWSLGLGVAEAMDTAQRGMGLDWPMSLELIRR
                     SLDAASDMPGALVASGCGTDQLVPETVKSVDEVIAAYEEQMAAIEKLGGRLIVMASRA
                     LARVARSPADYERAYDRILSQAAQPVILHWLGDMFDPALAGYWGTSDVDAAMDTALGI
                     IAAHSGKIDGIKISLLDKDKEIAMRRRLPPGVRMYTGDDFNYAELIAGDGVGSQPTHR
                     QSDALLGIFDAIAPAASAALAELAHGNPEKFHAILGPTVPLSRHIFAAPTRFYKTGVV
                     FMAWLNGHQNHFIMVGGQQSTRSLPHLAELFRLADAANLLEQPELAVKRMKTLLALHG
                     IDS"
     gene            complement(12108..>12793)
                     /locus_tag="VGA59_02865"
     CDS             complement(12108..>12793)
                     /locus_tag="VGA59_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9718645.1"
                     /translation="GRMLAVRLEFGYWVFEGDLQPIQRPSWNYRKEDGGGMILDMMCH
                     WRYVLDNLFGEVKAVSCIGATHIPQRWDESGKAYVATADDAAYATAELVGHNGEPVVA
                     QMNMSWATRVRRDDLVTFHVDGTHGSAVAGLTECRAQSRVTTPRPVWNPDEKQPMNFF
                     DQWQEVPDTQVYDNGFKIQWEHFIRHVVENEPYRWTLAEGAKGVQLVEAALQSWKERR
                     WVDVPALGI"
BASE COUNT         1945 a         4284 c         4347 g         2217 t
ORIGIN      
        1 cctgccgccg gagttcgccg ggcgggccga cgactacgtc gccgaagtgc tgcgtatgct
       61 gcccgtcctt cacggggaag gcctggtgga cgctgtcgac gtattctgcg aacggatcgc
      121 gttcagcgtg gcccagaccg aagcggtttt cgcggccgcg cgcgaactgg ggctgccggt
      181 caagttgcac gcggagcagc tcagcgacag cggcggagcg caactggccg ctcgctacgg
      241 agccctcagc tgcgatcatc tcgagtggct cagcgaggcg ggggcggctg cgatggcgcg
      301 gagcggctcg gtggcggtgt tgctgcccgg cgctttttat ttcctgcgcg acacccgcgt
      361 accgccggtg gagctgctgc ggcggcacgg cgtgccgatg gcggtggcta ccgattgcaa
      421 cccgggcagt tcgccttgta cctcgccgct gctgatgctg aacatggcgt gcacgctgtt
      481 caggctgaca ccgcaggaag ccttggccgg cgtgacccgc aacgccgccc gcgcgctggg
      541 cctgcgcgat cgcggcatgc tcgcggccgg cctgcgcgcc gacttcgtgc tgtgggacgc
      601 acagcggccg gcgcaactga gttacacgat gggattcaat ccacgacagc agacagtttt
      661 cgggggacgc tccttatgac cttcatcctg aaccgcggca cggcgccgct gctggtcagc
      721 atgccccaca ccgggaccga cataccgcag gcgttgcgcg aggatttcgt gccgcgcgcg
      781 ctcgccgttg aagacacgga ctggcatctg gcgcgcgtgt acaactgcct gccggcgttg
      841 ggcgccagcg tgctgcgtcc gcgttactcg cgctacgtga tcgacctgaa ccggccgccc
      901 gacgacatgc ccatgtatcc tggcgcgtcc aacaccgagc tgtgccccac gcggttcttc
      961 accggcgagc cgctgtatcc cgacgggcgc gacccttcgg cgcaagagcg gcagcgccgg
     1021 ctcgacacct attggcggcc gtatcaccag gcattgcgcg aagagctcga tcgcatcagg
     1081 gccgagcacg gcttcgcgtt gttgtgggac gcccacagca ttcgctcgga aattccgtgg
     1141 ctgttcgacg gcgtcctgcc cgacctgaac atcggaacag ccggtggcgc aagcgcgcac
     1201 gcgtcgatca cgcaggcggt ggcggatgca gcggcccgct acgccgacgt gagccacatc
     1261 gtcaatggcc gcttcaaggg cggctacatc acgcgccact acggcgatcc ggccagccat
     1321 gtgcatgcgg tgcagctgga gatgtgccag aacctgtaca tgaaagagaa accgccttat
     1381 gcgtatcagg aagcgctggc cgccgggatc cagccggtgc tggcggacat gatggccgcc
     1441 gcgctcgcgg cctgcggggg cttgtatggg cgctgagcag cagctctacc tcgcccctgc
     1501 ggcctgggtc aacggcgctt gggcgcagga tgtgctgctg acggccggcg ccgatggcca
     1561 gtggagcagc attgcgccca atgcgccggc cgacgcgcag cggggcgccc gccgcctgga
     1621 cgggccggtg gtgcccggcc tcgtcaatgg gcacagccac gccttccagc gtgctatggc
     1681 cgggctcacc gaacacagtg gtgccgccga ggacgatttc tggagctggc gcgaccgcat
     1741 gtacagcgcg gcgaaccgca tcaccccgga ccagctggag gcgatcgcgg ccttcctcta
     1801 ttgcgaactg ctgcgctctg gctacacgca tgtctgcgag ttccactacc tgcacaacgg
     1861 ccccgaaggt cggccgtacg ccgacccgcg ggagatgtcg ctgtgcctga tgcgggcggc
     1921 gcagcgcacg ggcatcggcc tgaccctgct gccgacgctc tacatgcgct cggggtttgg
     1981 cgcgaagact ttgcgcgaag accagtgccg ttttgcttcg actccggact cggtgttgcg
     2041 cattgccgag cttgtggcac gccagggcgg gcgggtggcg gcgggcgtcg ccgtgcattc
     2101 attgcgtgcc gtggatgagg tggcgctgcg tgaactggcg gcggtggcca ccggcatgcc
     2161 ggtgcatatc catatcgccg agcaaaggcg agaagtggac gactgcatcg agcaccacgg
     2221 ccttcggccg gtcgaatggc tgctggccaa ggtggcgttg gatgcgagct ggaatctcgt
     2281 tcattccacg cactgcaccc aagccgagct ggaaggcctg cgcaacaggc gcgcatccat
     2341 cgtgctgtgc ccgagtaccg aagcgaacct gggcgacggc atcttcgacc tgccggcctg
     2401 gatgggccag agcggaagct gggcgatcgg ttccgacagc catgtcacgc gcagctggca
     2461 ggaagaattg cgcctgctcg aatactcgca gcgcctcggc ctgcgccagc gcaatgtggc
     2521 cgcgcgggcc gccttgtgcg agagcagtgc cgccgcgctg ttcgagggtg ccttggaagg
     2581 cggtagcgcg gcagcgggcc ggccactggg gggcctgatg gcgggccagc gcgccgattt
     2641 tctggtggtg gatgccacga gcagtgcatt ggcgggcata ccgccggacc atctgctgga
     2701 cgccctcgtc ttctccagcc cggcgcccgc gttcagcgag gtcttcgtcg cggggcaagc
     2761 ggtgcccgca gctgatcccg cggtacgcgc gggcttcgtg cgcgcgatga aagagctctg
     2821 gacctgacag ggacctggaa cgccggtcgg tcctggggtc gcggcagacg gcacccccgc
     2881 ctttgccgcc atggctgccc caaacgctgc agctcgactg cgcagccttt gatgagaatc
     2941 aatatattga caaacatatt gtgattcggc aaactgaggt gcgctggatc aaagacatga
     3001 aacgcgaaga catggttgaa accgccgcga tcgacctgga cgtcatgcgc cagggcgcgc
     3061 agggggcatg cgcgttgatg aaagtgctgg ccaaccccga ccggctgctg ctgctgtgcc
     3121 agattgccga cggcgagcgc aacgtgggcg aactggaaga agcgctgggc atcgggcagc
     3181 ccacgctgtc ccagcaactc accgtgctgc gcagcgaatc gctggtcgcg acgcgacgcg
     3241 acggcaagaa catctattac cggctagcca gtccgcgggc catggccatc atgcaggcgc
     3301 tgtatgcgca gttctgcgca agcgccaaac ccaaaaggag ccgccaatga cgatcgattg
     3361 gaaccacttc acgccctggg cctcgctggc cggaggcctc ttgatcgggc tcgcctcgac
     3421 gctgtttgtc ctgctcaacg ggcgggtgct gggtatcagc ggcatcctgg ggggcctggt
     3481 gaagccgcgt acgggcgacg cgctctggcg catcgcattc atcgcgggct tgctcgcggc
     3541 tccgacgctg tggctggcgt ttgcgcagcc cgtggcaccg cgcatcgagg cgaccggtgc
     3601 catgctcgcg gccgccggcc tgctggtggg ctgggggacg cgctatggtt cgggctgcac
     3661 cagcggccat ggggtatgcg gcctctcgcg gctgtcgcgg cgctcgctcg cggcgactgt
     3721 gacgttcatg gccgccggct tcgccaccgt gttcctcgtt cgccatgttt tcgcctgaag
     3781 gagccgccat gaaccgattg atcgaattca tcgccggcct cgtcttcggc atcggcctca
     3841 tcctctcggg catgaccgat cccggcaagg tgatcggttt cctggatctt gccggaacct
     3901 gggacccgtc gctcgccctg gtgatggcgg gcgcgatttc cgtgggggtg ttcgggttcg
     3961 gcctggcccg caagcgcagc acgaccttgc tgggtggccc catgcagttg cccaacaccg
     4021 acgtcatcga ttcgcgcctg ctgatcggca gcgccgtgtt cggcgcgggc tggggcttgg
     4081 cgggcttttg tcccggtccg gcggtggtcg cgaccggggc tgggcattgg caggccctgg
     4141 tgttcacgct ggcgatgctc gccggcatgg ctgtgcatga gctgagcgcg aggccggcac
     4201 cgcctgcgct ggtgcgggag tagccccgcg tgccgcctgc tttgctggct cctggcctcg
     4261 gcgtgctgtg gtcgggcctg gcggggccgc gtttgcagca gggcttcgcg ttgctctcga
     4321 tgggtgttgc ctgcgccttg ttggcgagga tcgcggtcta gcttagctac tttccgtcga
     4381 agcgtcgcct tgatgacttg cgcgggaaac ggctcggcag aaattctccg cgccgcttag
     4441 gatcggggct tatgagcaca atgaccgcga gcgcgccgcc cgccaccagc ttgcgccagg
     4501 atgcgggcgt gatcggcctc gtcggcctgg cccacatgat cagccactac ggacagctct
     4561 tgctcgcgcc gctgtttccc tggctgaagg atgccttcgg tgccagctac acggagctgg
     4621 gtttcctgat gacgctgttc ttcgtggtgt cctgttcggt tcaggcggtc tcgggtttcc
     4681 tggtggatcg gtatgggccg cgtccgatcc tgttcggcgg gctggcgctg ctggccacgg
     4741 cagctttcgg ctacgcgctc agccgcggct attggacgat ggccattttc tcggtgctcg
     4801 ccggcgtggg aaacggcgtc ttccatccgg tgaactacac gctgctcaac cgcaacgtca
     4861 gcccgccccg gctgggccac gcgtacagct ttcacggcat taccggcagc ctcggctggg
     4921 ctctggcgcc cgcgatgctg gtgcctctgg ccatcgtctt ttcctggcgt accgccttga
     4981 tggccgccgg agcgttggcg ctcgcggtgc tgggggtgat gtggctgtac cgggcccgcc
     5041 ttgcgttgcc ggccagcgct cccaggcatg cggcagcggc ccacgcggag ggcactttcg
     5101 aatttctgcg gatccccgcc gtgtggatgt gcttcgcttt tttctttttc tatgccgtgg
     5161 tgctgagcgt cattcaggcg tttgcaccgg aagccgcgcg gcagttgcac ggcgtgccgg
     5221 tggctctggc cgccatgtgc cttacggttt atatggtgtg cagcgccggc ggcatggtac
     5281 ggggtggctt tctggcctca gacccggcgc gctgcgagcg ggtcgtcggc atgggcttcg
     5341 gggtcgcggc gctggttgcg ctggtgatcg gcgtgggtac cgtgcccgca ctggcggttc
     5401 ccgcgctgtt cggcgcgatg ggtttcgcga gcggcatcgc cgggccttcg cgcgacttgc
     5461 tggtcaagcg ctccaccccg gagaacgcga ccggccgcgt ttacggcatc gtctattccg
     5521 gcctggacat cggccaggcc atttcgcccc tgattttcgg ggccctgatg gaccaccagc
     5581 agtaccgggg cgtctggctg gggctggtgt tcatgcaggc ggtgctgatc accagtgcgt
     5641 tcaatgtgcg acgggtgcgg cgtacggcgc tgacgaccgc ctgaagcgcc gcccgcgcga
     5701 ggagttatcc ttgcggccat gtacgcgtcc tatttcggcc tgaagtaccc gccgttttcg
     5761 atcgcaccgg acccccgcta cctgttcatg agcgagcgcc accgcgaagc gctggcgcac
     5821 ctgctttacg ggctggatgg cggcggggga tttgtactgc tgtcgggcga gatcggcacc
     5881 ggcaagacca cggtctgccg gctgttcctg gaacaggtgc cggccaactg caacgtcgcc
     5941 tacatcttca accccaagct cacggccgcg gaactgctgc agtccgtctg cgacgagttc
     6001 catattccgg tgcggcaaaa ggacggtctg ccgcccacgg tcaaggacta cctggacccg
     6061 ctcaacgcgt tcctgctcca ggcgcatgcg gccggacgga acaacgtcct gatcatcgac
     6121 gaggcgcaga acctgtcggc cgaggtgctg gagcagctgc gcctgctcac caacctggaa
     6181 acctccgagc gcaagctgct gcagatcatc ctgatcggcc agcccgagct gcgcagcatg
     6241 ctggcccggc ccgaactcga acaactcgcc cagcgggtga tcgcgcgtta ccacctcgac
     6301 gccctgacgc cggcggagac cgcgcagtat gtccagcacc gctgcgaagt cgccggcctg
     6361 agccggccgc tgccgttcga gcgcaaggcc ttgcggcgcg tccaccagct cgcgcgtggc
     6421 gtgccgcggc gcatcaacct gctgtgcgac cgcgcattgc tgggcgcgtt cgcaaacagc
     6481 caggcggggg tggaccgcgc gaccatcgac aaggccgccg ccgaggtgtt cgacacgccg
     6541 gcgagagccc cggtcgcgtg gcgccgcgcc gcagccatcc tgggtctcgg gctggcggcc
     6601 ggtgccgggc ttttcgcgac cgccaatcat ctgacgcagg acgcggcctt gcccccgctt
     6661 gcgatccagc cggcccccgc gcctgctgag gagcaagcgg tggccgcgcc cgtggccgag
     6721 gttgacgctc gccaatcccc ggcaacagcc gcgccgccgc cggccctgct gcacgaccgg
     6781 gacgaggcat ggcgcgaact cgcgctcagc tggaacgcgg aagcgggcga gggcgagcca
     6841 tgcaaggcgt tgcaaaaaga tcaattgcac tgcttcagca agaatctcaa cctggcgctg
     6901 atccgcgagc tgggccggcc cggcgtcctg acgctggacg caggcaccgg cgcgccgacc
     6961 tatgccgtcc tcaccgcctt gacgcgcgac agcgcgacct tgcgcgccgg cggcaccgag
     7021 cagacggtga ccctggccgc gctggccgcc cgttggcagg gcgatttcgc cacgctgtgg
     7081 cgggtgccgc cgggctatac ggcgcgaatc gggagcggcc tcagtggcga aaccctggaa
     7141 tggatcgccg ccggcctggc cgctgtcgac ggctcgaccg ttccgtccgg gcggcgcgcg
     7201 ccggacgcgg cggtgctgaa aacgcagctg cgctcgtttc agctggccca gggcatggtc
     7261 ccggatggcc aggtgggccc gatgactttc atgcagctca atcgcgtggc cggcgttgaa
     7321 gagccgcgcc tgcgcacgga accttagtgt cctgtcccgc aaaataaggt gatgaaatga
     7381 aattgtcctt ttggctgtca ggcaaggcgc gaggaggcga cattgccgca gcttcggcaa
     7441 ctacgagtaa cgccgcatgg cggccaggtg gacgatttca tatcgccttt gtttgcggga
     7501 caggacacta gccatgtctt atatcctcga cgccctccga aaagccgatg cacagcgcga
     7561 acgcgacccc gcgcgtggaa ttcacgccca gccggtgcgc gcgtcgatcg gcgaacggcg
     7621 aagccgcgac gcgaatcgca tcgggttctg ggcagcaacc gcggcggtgc ttgcggcgct
     7681 ggcgtcggtt ggggggtatc tgtaccgcga cagtggtgcg ccgccggcgc ccaccgaagt
     7741 cgctgtggcg gcgcctcccg tgacgatcgt tcccccggca gctcccccgg cagctccccc
     7801 agccgcggag cctgccgctg ccgtgttgcc gccgccggtc gtggtcgcgc caccgcctgt
     7861 cgtcagggca cccgaagcca ccgccgcgcc cccagtcgcg gcgcgtcgcc ccatcaccaa
     7921 gcaggttcaa ggagtgcctt tcgaacccac ggtggcccgt aaggggtcca ggcattcgcc
     7981 tgcgggcgct cctgtagcga cgcgcgcgcc gccggcgcct gtccccgcgc agccggtacc
     8041 cgaccccgcc ccatcggtgg cgaccgtggc tccacgggtg gcggccgtgg tttcaccggc
     8101 ggcgaccgtg gctccacggg tcgcgtccgt ggctccaccg gcggtggctc catcgcctgt
     8161 cgcggagccc gttgcggggg gcctgccggc agacgctccc aagctggcga tcagcggagg
     8221 ggtgtactcc gccaatgtgg ctcagcgcat gctcatcgtc aacggccagg tcttcaatga
     8281 aggcagcgag gtcggagccg gcgtcgtgct cgaggaaatc aggcccaagg ccgcggtgct
     8341 caggttccgc ggttcgcgtt acacggtcgc ctattgagca ttgcccgcaa gagcgcgccg
     8401 cctagggcct gtgctaatgc gcctgcggcg gctggaatac cgaccagcgg ccatccttga
     8461 acagataaag agccgaagat aggcgcgcgc ccgggccgcc cgcgtagagc gcggccgcac
     8521 tgaagcgcag gttggtgacc gccgcttcgt ccgaagccgg cgcgtagacc aggccgccgc
     8581 ggcgcgggac cgccaccacc agcccttccg gatgccggta ctgcagtgcg cgccagaact
     8641 cgcgatcgag aaagtagctg ctattgaggt cggccgcgtc tccgtgcacc tgcatcaggt
     8701 tgccgctcca cggttggacg gcgggagctc cgtagacccg ccgcaggttt gcgatggcca
     8761 gctccagtgc gtcctgcgga ctcaggctca ggcgtgccag gtcatcgggc gaggcgcttt
     8821 gcatcgtctg tccgccgtcg aacaccagcc ggatgtgcat gtcgcccagg tactggtacc
     8881 agctggtttc cagctcgatc tggcggccgt cgcccaaagt tgcacgcagc gtgccgaaag
     8941 gctccagcga ctcgtcctgc ttgttctcgc ctcgcgattc gcgcagcact accagcaggt
     9001 tcgcggcact tgtgtcgagt ggctcgacgc ccggcgcggc ggctgcaagc gactcgagcg
     9061 cggcgacagc caatagcagg cagaacctga agacccggag gagtcggctg cttgtgttca
     9121 tcgtgccctg cgtcctttgc ttggattccg caatcgtgcg tcacagctga ggcagccgca
     9181 acagtcattt acgcaaaccg ggtaatatgt ctcgaacgac gggggcacgc gtcggtacca
     9241 ggccacgaac ttcgttaccg atcttgctca agacggtgga catgtaaggg cagtcatccg
     9301 tagcatcggc agctctttca acgccaccca gaggaagtac atcatggcac tgcaatctcc
     9361 cttcttcaac aagcgcgaac ccgacgcgta cagccagcgg aacaatccca accccaacag
     9421 cgcccccgcc acgaccaccc cggcgcagca gccggctaac gcaccgattg ccgccaccgc
     9481 cgccgccaac agcgcgcccg cttcgaccgg ccaggaaggc gaaagcaaac tgaccgtcgg
     9541 gccgaacatc aagctcaagg gcgtcgaaat cacggattgc gacacgctgg tggtggaagg
     9601 ctccgtggag gcgaccatgg actcgcgcgt gatccagatt tccgagcgcg gctcgttcaa
     9661 gggctcggcc gacatcgaca tcgccgagat ccgcggccag ttcgacggta acctgacggc
     9721 gcgccagaaa ctcgtgatct acgccaccgg caaggtgacg ggccgggtgc gctacggcaa
     9781 ggtggtgatc gaagagggcg gccagctgtc cggtgaagtc gaattcggca ctgccaccag
     9841 ccggtccgct tccgccggcg gtccgcgccc cggtctgcag gtagcaacgg caaaggtctg
     9901 acgctccagg gcgggggttc ccgcccgctc acaccgcgcg gcggtgcctc tcgatgcagg
     9961 tgtccagcac ctcgccgtga tcgcgccgcc accagttgtt gctcgagaat atctcgactt
    10021 cgctgtagcc gccgaaaccc tggccctcga gccagccgcg gatgcgcgga atatcgatca
    10081 cgccatcgcc catcatgccc cggtcgttga gcaggtcggt ggtcggcgtg agccagtcgc
    10141 agacgtgaaa ggccagcagc cggcgcttgc cggcgcgctc gatctgcgcc ttcagcttgg
    10201 ggtcccacca cacgtggtag acgtcggcgg cgacgcctaa cgccgccgta gctgttcgcg
    10261 gctcgccgcc tgatgggccc gggtccagtt catcgcatag gtccagtgcc tgctcgaggg
    10321 tgttgatgca cgcgcgctcg gccgcgtaca tcgggtgcag cggctcgatc gccaggggca
    10381 tgtgcatgga ccgggcgtag tccagcagct cgccgattcc atcgcgcacc tggctgcgtg
    10441 ccagggcgat gtccttgtgc gcgggcatgc ccgccagcgc gccgggcagt ccccccacca
    10501 ccaggaccag gcaggccgct cgcaattcgc aggcttcgtc gacagctcgc cggttatcgt
    10561 cgcgcgccgc gcgcaggccc tgggcatcga ccgccgggaa catcccgcct cggcaatagc
    10621 ccgagagttc catgccatga gtcctgacga gccgggaaat tgtgtcaagc cccgcagccg
    10681 ccacctggtc gcgccagggg gagatcgcac ggatgccccg gcgcgcgcag gcttcgatga
    10741 tccgcggcaa cggcaggtcg acgccgcggc ttttgcgcac ggtggcggtg ttgatggaga
    10801 gccagcggtg atcctgggag aagtcgcgca tggttcctgg cggtcattcc cggttcttgg
    10861 ccgtcatccc gggctcgacc cggtcaagcc cgcaatggca actgttttca tgaatctatc
    10921 ccgtgcagcg ccagcaacgt cttcatacgt tttaccgcaa gctccggttg ctccagcagg
    10981 ttggccgcgt cggccaggcg gaacagttcg gccaggtgcg gcagagaacg cgtgctttgc
    11041 tggccgccga ccatgatgaa gtggttctgg tggccgttga gccaggccat gaacaccacg
    11101 ccggtcttgt aaaagcgcgt cggcgccgcg aagatgtggc gcgacagcgg cacggtgggg
    11161 ccgaggatgg cgtggaattt ctccgggttg ccgtgcgcca gttcggccag cgccgcgctc
    11221 gccgcgggcg caatggcgtc gaagatgccc agcagcgcgt cgctctggcg atgggtgggc
    11281 tgtgagccga caccgtcgcc ggcgatcagt tcggcgtaat tgaagtcgtc gccggtgtac
    11341 atgcgaacgc ccggcggcag gcggcgtctc atggcgattt ccttgtcctt gtccagcagc
    11401 gatatcttga tgccgtcgat cttgccggaa tgcgcggcga tgatgcccag cgccgtgtcc
    11461 atggcggcat ccacgtcgct cgtgccccag taaccggcca gcgcggggtc gaacatgtcg
    11521 ccgagccagt gcaggatgac gggttgcgcc gcctggctga ggatgcggtc gtaagcgcgc
    11581 tcgtagtcgg ccgggcttct tgcgacgcgc gccagcgcgc ggctggccat gacgatgagc
    11641 ctgccaccca gcttttcgat cgccgccatc tgttcctcgt aagccgcgat gacctcatcg
    11701 acggacttca cggtctccgg caccaattgg tcggtgccgc agcccgaggc gacgagcgcg
    11761 ccgggcatgt cgctggctgc gtccagcgag cggcggatca gttccagcga catcggccag
    11821 tccagcccca tgccgcgttg ggcggtgtcc atggcctcgg ccacgcccag gcccagcgac
    11881 cacaggcgcc ggcgataggc gatggtggcg tcccagtcga tcgagcattg cagccaggga
    11941 tcgatcgccg cccgcggatc ggccacgaca tgcgcggccg agtaggcgat gcgattgaat
    12001 cggaccccgg cggcgggctt caccggttcg gcgccgcgca gcgtgtactg gaacaggtcg
    12061 ccgccggtcg agggcaattg cagtgaaaga gccatggcag ccccccctta gatgcctaag
    12121 gcgggcacgt cgacccagcg ccgctctttc cagctttgca gcgccgcctc gaccagttgc
    12181 acgcccttgg cgccttccgc gagcgtccag cgatagggtt cgttctccac gacgtggcgg
    12241 atgaaatgct cccactggat cttgaagccg ttgtcgtaca cctgtgtatc cggcacttcc
    12301 tgccactgat cgaagaagtt catcggttgc ttctcgtcgg gattccagac cggcctgggt
    12361 gtggtcacgc gggattgcgc acggcactcg gtcaggcccg cgacggcgga gccatgcgtg
    12421 ccgtccacat ggaaagtcac caggtcgtcg cgccgcacgc gcgtggccca ggacatgttc
    12481 atctgcgcca cgaccggttc gccgttgtgg ccgaccagtt cggcggtggc ataggccgcg
    12541 tcgtcggcgg tcgccacata agccttgccg gactcgtccc agcgctgcgg gatgtgcgtg
    12601 gcgcctatgc acgagacagc cttcacttcc ccgaacaggt tgtccagcac atagcgccag
    12661 tggcacatca tgtccaggat catgccgccg ccgtcttcct tgcggtagtt ccagctcggg
    12721 cgctggatcg gctgcaggtc gccctcgaac acccagtagc cgaactccag gcgcaccgcg
    12781 agcatgcgac cga
//
LOCUS       DASWIY010000075         9112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2633592, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000075 DASWIY010000000
VERSION     DASWIY010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9112
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2633592"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..3329
                     /locus_tag="VGA59_02870"
     CDS             270..3329
                     /locus_tag="VGA59_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9718646.1"
                     /translation="MPRPAALPSASASGWGELKQATVLFADIVSSTEQIAQLDPEEAM
                     DRLAPAVSLMCDAVERFGGTVIRTLGDGIMALFGVPKALEGHARLACESALHMQAAFA
                     RNPQDLSIRVGLHSGLVASDPQAHDSGKGGGAHGLTIHLASRVVGVAEPGGVAITADC
                     HSLVRGSVDVESLGHPALKGIPEAVEIFRLVVLKAAFASQHFHQAKLSPFRGRDKELA
                     LLQHAQGLAEAGNAQVIGVCGGPGTGKSRLCYEFAQWCRARGRPVFEVRAQLYGSATP
                     LQPVLELMRIFFFGISETDSPAAARARIAARLDLLGSGEPRDQALLSDFLGVPDPGDS
                     MAPPLNAKARRTRLLAIMSALVKQNADEASVVLIEDLHWLDEASEEFVAALVKAVAAT
                     RTMLVLNYRSSYRSPWVGIGNFREIEVGELSAADTDALVRELISHRREFQDISQLIVR
                     RSGGNPFFAEELVRSLAERGVLSGDPAHSNNGIGTVDRALPATVQAIIGARIDRLSGS
                     EKSLLQICAIIGKEIPLAILERVAGPMRGEIERALDALCHVELIQPQPASGGRRFAFQ
                     HPLIQEVAYSTQLKARRSAVHASVASAMEDHYADQLDEYSALISHHYEAAGHAPSAAE
                     YSARAAKWLSSTDSAQAIKHWRKVRALLENQPRNQQSDRLKAMACSQISLLGWREGLS
                     LQEVQPFIEEAMQLADKVDGRLVQFLLMIEGRMTQASGGPADWYIEKVAQALAMADAQ
                     SDLGRIATLNAALSQAYGWAGLLDKALAANDVVLAHADKIESFDHDFMGFNVEHWMLG
                     MRGRLLIRLGRFDEAHEHLRRLLALGDALVDPVLLQIVHYTFVDLACCNGDAPLAQEH
                     AAIVSEIADRHRNPYMRVMALSGRAMALSVVGDFDAAAVTLSEAVETIRGSNVSLELE
                     PEVLANLAECHRRLGNLELALLLAKEAIDVSRQRSTRLAECRALISHAALLVDGRQTT
                     QLAEARALLDAAAELIGVTGARIYDALLERERKRLEELVGEEKSLTP"
     gene            complement(3315..3956)
                     /locus_tag="VGA59_02875"
     CDS             complement(3315..3956)
                     /locus_tag="VGA59_02875"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9718647.1"
                     /translation="MDKSRTRETTLGEEVERYYSDQLRRYGATPLGVGWTCAPTQDLR
                     FVQLARLFDGARGFSLNDVGCGYGALLGYLRKRHKGIRLDYLGLDLSQAMVDAAKRKW
                     RKVPGAAFALAGNSYRMADYCVASGIFNVKLNQDRRTWEDFVAGTLVEMYENSRLGMA
                     VNFLREDGALEEVPELYRCRADGWRDFGRQLGAAVEINGDYGMSEFTLLLRRQ"
     gene            complement(3973..4491)
                     /locus_tag="VGA59_02880"
     CDS             complement(3973..4491)
                     /locus_tag="VGA59_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718648.1"
                     /translation="MGLFMKEHDVHVENLLGELNVRFGPTPNKKSHFGGIAEMAALQK
                     EFKIFKKGRSFRTSVSVLNIGATNAEARNRLLDYFGNLSKHDSNVAGQNGDVAIVNAI
                     VRNLSSKNPLPVYFTYHDMRAEKGNTRVLITDKSRPVPFFQQDYLTISVPTKAHATAK
                     KTAKAAPKAPKA"
     gene            complement(4780..5925)
                     /gene="hppD"
                     /locus_tag="VGA59_02885"
     CDS             complement(4780..5925)
                     /gene="hppD"
                     /locus_tag="VGA59_02885"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX9718649.1"
                     /translation="MNARLPEQAPARTDAWENPMGTDGFEFIEYAAPDPKAMGELFER
                     MGFKPIARHRHKNVLLYRQGGINFILNAEPDSFAQRFARLHGPSVCAIAFRVKDAKAA
                     YGRAVSLGAWGYAGQAGPGELNIPAIKGIGDSIIYLIDRWRGKNGAKEGDIGNIGFFD
                     VDFEPISKDSGAELNPVGIGLTYIDHLTHNVHRGRMEEWAGFYERLFNFREVRYFDIE
                     GQATGVKSKAMTSPCGKIRIPINEEGNEKSGQIQEYLDRYHGEGIQHIAMGSADLYET
                     VDALQMSGVKLLTTSETYYELLPKRIPGLRENIAELQQRNILVDGKPDELLLQIFSEN
                     QLGPIFFEFIQRKGNEGFGEGNFKALFETMELDQMRRGVLDKKQPAA"
     gene            6196..6675
                     /locus_tag="VGA59_02890"
     CDS             6196..6675
                     /locus_tag="VGA59_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9718650.1"
                     /translation="MESHALDKLDKAILRCLQQNGRETYDVIGAQVGLSSSAVLRRVK
                     RLEEAGVIDRYVALVKPEAVGLGLTAYLNVRLEKHTESHKRNPMDLFRASVQTWPEVV
                     ECAALTGEMDYLLRVVVQDMAHYRRFIMDTLLKHPSVQDCKTSFVLDRVKATTAMPL"
     gene            6832..7488
                     /locus_tag="VGA59_02895"
     CDS             6832..7488
                     /locus_tag="VGA59_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9718651.1"
                     /translation="MTESEELTKTPVNVPIRSIGPSHRDRITAHLMALDEHDRYLRFG
                     YAATDEQVQRYVASLDFERDEIFGVYNRRLELIAMAHLAFSVDPALRACAEFGVSVVK
                     KARGRGLGARLFDRAVMHARNEGVEMLFIHALSENTAMLKIARNSGATLERAGSETDA
                     YLRLPPATLDSRMSEILAEQLAQTDYRLKAQAKSFWDFISGVQEVRQGVRDARHKSGG
                     "
     gene            7621..8499
                     /locus_tag="VGA59_02900"
     CDS             7621..8499
                     /locus_tag="VGA59_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEX9718652.1"
                     /translation="MSDPHPARASAEKEDRRTFLQKIAEFIHPGPDSKAELIETLADA
                     EDNDIIGAESRVMLEGVIRMAELTAGDVMVAAPRMDLLNIDSPYDEILEIVIDTAHSR
                     FPVYEGDRENIIGILLAKDLLKLQRAPELNIRALLRPAVFVPETKGLNDLLREFRGNR
                     NHLAIVIDEFGRVAGLITIEDVLEQIVGEIEDEFDIDEDEGDIFGLADRTYRVSGDTA
                     VERVGEAFGVTLEPTDADQEFDTIGGLIAHEMGHVPKRGEHYTLGGLNFVVMHTKGGA
                     VRWFKVSPTKDDDHTG"
     gene            8616..>9112
                     /locus_tag="VGA59_02905"
     CDS             8616..>9112
                     /locus_tag="VGA59_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX9718653.1"
                     /translation="MFLLALALLAGLLQAASIAAPWSGQPAWWLQLLSLAVLAWLAQQ
                     APSWRRAGLLGWTFATAWLAGTFWWLFISMHVYGGLPAPLAAFAVLALAAFLATYYGA
                     ACAVFALVRGSTRLPGALAFAALWLLAELLRVTWFTGFPWGAGGYAHVDGPLSFLAPQ
                     VGVHG"
BASE COUNT         1587 a         2849 c         2989 g         1687 t
ORIGIN      
        1 ggcgctacat ggggcgcgat ccgcgaatca tgaagccgtc aaggcggcat ggaagaaggt
       61 gaacgtttta gtctgatagc cgagcagcgt actattcggg catggaatgc ccgtcctgtc
      121 acacggcaaa tcccgaaacc aaccgcttct gtgaaaactg cggccaagcg ctgccgtaca
      181 agtgcgcggc ctgtgggttc gactgcgcgc ctcaggcgag attttgtggc ggctgcggag
      241 cttccctgct ccgggggccc tcgtttgagg tgccacgccc cgccgcgctg ccttcggcgt
      301 cggcctcggg ctggggcgag ttgaagcagg ccaccgtcct ctttgccgac atcgtcagtt
      361 cgaccgagca gatcgcccaa ctcgatccgg aagaagccat ggaccggctg gcgccggcgg
      421 tctcgctcat gtgcgatgcc gtcgagcggt tcggcggcac ggtgatccgc acgctgggcg
      481 acgggatcat ggccctgttc ggcgtgccca aggcgctgga aggtcatgcc aggttggcgt
      541 gcgagtccgc gctgcacatg caggcggcgt tcgcccgcaa tccgcaagac cttagcatcc
      601 gggtcggttt gcactcgggc ctggtggcct ccgacccgca ggcgcatgac agcggcaagg
      661 ggggcggcgc gcatgggctg acgattcacc tggccagtcg cgtcgtgggt gtcgccgagc
      721 ccggcggcgt ggcgatcacc gcggattgcc attcgctggt gcgaggttcc gtcgatgtcg
      781 agtcgctcgg ccaccccgcg ctcaagggca tcccggaagc agtggagata ttccggctgg
      841 tcgtcttgaa ggcggctttc gccagccagc atttccacca ggccaaactg tcgccgttcc
      901 gtggccggga caaagaactg gctttgctcc agcacgccca aggcctggcc gaagccggca
      961 acgcgcaggt cataggcgtt tgcggtggac ccggcacagg gaaaagccgg ctttgctatg
     1021 agttcgcgca atggtgccgt gcgcgtggca ggccggtgtt cgaggtccgc gcacagctct
     1081 atgggagcgc gacgccgctg cagcccgtcc tggagctgat gcgcatcttc ttcttcggga
     1141 tatccgagac ggattcgccg gccgcggcgc gcgccaggat agcggcgagg ctcgatctgc
     1201 tggggtccgg cgagccgaga gaccaggcgc ttctgagcga tttcctgggc gtgccggatc
     1261 ccggcgattc catggcgccg ccgctgaacg cgaaggcgag gcgcacgcgc ctgctcgcga
     1321 tcatgagcgc attggtcaaa cagaatgcgg atgaagcatc ggtcgtactg atcgaggacc
     1381 tgcactggct cgacgaggcc agcgaggagt tcgtcgccgc gctggtgaaa gccgtggccg
     1441 cgacgcggac catgctggtc ctgaactacc gttcgtccta ccggtcgccc tgggtcggga
     1501 tcggcaactt ccgggaaatc gaggtcggcg agttgagcgc cgccgatacc gatgcactgg
     1561 tgcgcgagct gatcagccac cggcgcgagt tccaggacat ttcccagttg atcgtcaggc
     1621 gcagcggcgg caatccgttc ttcgccgagg aattggtccg atcgctcgcc gaacgcggcg
     1681 tgctgtcggg tgacccggcc cattcgaaca acggcatcgg caccgtggat cgcgcgctgc
     1741 ccgcgaccgt gcaggccatc atcggcgcgc ggatcgatcg tctgagcggt tccgaaaaat
     1801 cgctgctcca gatatgcgcc atcatcggca aggaaatccc gctggcgatc ctggagcgcg
     1861 tcgcaggacc gatgcgcgga gaaatcgagc gcgcgctcga cgcgctgtgt cacgtggaac
     1921 tgatccagcc gcagcccgcc tccggagggc ggcgctttgc cttccagcat ccgctgatcc
     1981 aggaggtcgc ctatagcacg cagctcaagg ccaggcgctc ggcagtccac gcttcggtgg
     2041 ccagtgccat ggaggaccac tacgcagacc agctcgatga gtattcggcc ctcatctccc
     2101 atcattacga ggctgcgggc cacgcgccga gcgcggcgga gtactcggcg cgtgcggcga
     2161 agtggttgag ttcaaccgat tcggcgcagg ccatcaagca ctggcgcaag gtgagggctc
     2221 tgttggagaa tcagccgcga aaccagcaga gcgaccgcct taaagccatg gcatgcagcc
     2281 agatttcgct gctcggctgg cgggaaggct tgagccttca ggaagtccag cccttcatcg
     2341 aagaggcgat gcaacttgcg gacaaggtgg acggccggct ggttcagttc ctgctcatga
     2401 tcgagggtcg tatgacgcag gccagcggtg gccccgcgga ctggtacatc gagaaagttg
     2461 cacaggcgct cgccatggcc gatgcccagt cggacctggg gaggatcgcc acgctcaacg
     2521 ccgccctgag ccaggcctat ggctgggccg ggctccttga caaggcgctg gctgccaacg
     2581 acgtcgtgct tgctcacgcg gacaagatcg aaagttttga ccatgatttc atgggcttca
     2641 acgtcgagca ttggatgctg ggcatgcgcg ggcgcttgtt gatccggctg ggacgtttcg
     2701 atgaagctca cgaacacctt cgccggttgc tggcccttgg tgacgcgctg gtcgacccag
     2761 tgctcctgca gatcgtgcac tatacgtttg tcgacctggc gtgttgcaat ggggacgccc
     2821 ctctcgcgca ggagcatgcc gcgatcgtga gcgagatcgc ggacaggcac cgcaacccgt
     2881 acatgagggt catggctcta agcggccgcg ccatggcact cagtgtcgtg ggggacttcg
     2941 acgcagccgc ggtcaccctt tccgaggcag tcgaaacgat acgcggttcc aacgtgtcgc
     3001 tcgaactcga acctgaggtc ctggccaatc tggccgagtg ccacagacgg cttggcaacc
     3061 ttgaactggc gctgctcctg gccaaggagg ctatcgacgt gtcaaggcag aggagcaccc
     3121 gactggctga atgccgggcg ctcatcagcc atgccgcgct actggtcgac gggcgccaga
     3181 cgacacagct ggccgaggcg cgggcgctgc tcgacgccgc agccgagctc atcggcgtga
     3241 cgggtgcgag gatctatgac gctcttctcg agagggagcg aaagcggctg gaggaattgg
     3301 tcggcgagga aaaatcactg acgccttagc agcagcgtga attcggacat tccatagtcg
     3361 ccgtttatct ccacggcggc gcccagctgt ctgccaaaat ccctccaacc atccgcgcgg
     3421 caccggtaca gctcgggcac ttcctccaag gcgccgtcct cccgcaggaa gttcaccgcc
     3481 attcccagcc ggctgttttc atacatttcg acgagcgtcc cggcgacgaa gtcctcccag
     3541 gttcggcggt cctggttcag cttgacgttg aagatgccgc tggcaacgca gtagtccgcc
     3601 atgcggtagc tgttgcccgc aagagcgaac gcggctccgg gcaccttcct ccactttcgt
     3661 ttcgccgcgt cgaccatggc ttgggaaagg tccaggccca ggtagtcgag tcggatgcct
     3721 ttgtgccgct tgcgcagata accgaggagc gcgccatagc cgcagccgac atcattgagg
     3781 gaaaatcccc gggcgccatc gaacagcctg gcgagttgga cgaagcgtag atcctgtgta
     3841 ggtgcgcaag tccaaccgac gccgagcgga gtggccccgt accggcgcag ctgatcactg
     3901 taatagcgct cgacttcctc gcccagcgtc gtttcccgcg tgcggctttt gtccacctcg
     3961 accccgcggg tctcaggcct tcggggcctt gggagcggct ttcgctgttt tctttgccgt
     4021 ggcgtgggcc ttggtcggaa cggagatcgt caggtagtcc tgctggaaga agggcacggg
     4081 ccggctcttg tcggtgataa gaacgcgggt gttgcccttt tccgctcgca tgtcgtggta
     4141 cgtgaaatac acgggcagcg ggttcttgga cgacaggttg cggacaatcg cgttgacgat
     4201 ggcgacatcg ccgttctgtc ccgccacgtt ggaatcatgc ttgctcaggt taccgaagta
     4261 atcgagcagc cgattgcgtg cctcggcgtt ggtagcgccg atattcagca ccgacacgct
     4321 tgtcctgaat gagcgaccct tcttgaaaat cttgaactcc ttttggagcg cggccatttc
     4381 cgcgatgccc ccgaagtggc ttttcttgtt gggcgtcggg ccgaatcgca cgttgagttc
     4441 tccgaggaga ttttcgacgt gcacatcgtg ttctttcatg aaaagaccca tggctttcac
     4501 tcctgtgtgt ttactggtaa ggcggcgtta catccgtaag gatttacgcc ggggcagtgt
     4561 aaacacaaag tccggatctt gagaagagcg aggagggcta tctcctccac attgaggagt
     4621 gagagggcgg atgggcgggt gagagatggt gcgccgcggt gccgacgctc ggcgccaagg
     4681 agcgcattcc ccgcttcgac gagatcaacg agggcctgca ccaggctact cgctgtcaag
     4741 ccggggatgg caactcgggg gccaagcccg cagtgactat caggctgcgg gctgcttctt
     4801 gtccagcacg ccgcggcgca tctggtccag ctccatcgtc tcgaacagcg ccttgaagtt
     4861 gccttcgccg aagccttcgt tgcccttgcg ctggatgaac tcgaagaaga tcgggcccag
     4921 ctggttctcg ctgaagatct gcagcagcag ttcgtcgggc ttgccatcga cgaggatgtt
     4981 gcgctgctgc agctcggcga tgttctcgcg cagccccggg atgcgcttgg gcagcagctc
     5041 gtaataggtc tcgctggtgg tgagcagctt cacgccgctc atctgcagcg cgtccacggt
     5101 ttcgtagagg tcggccgagc ccatcgcgat gtgctggatg ccctcgccgt ggtagcggtc
     5161 caggtactcc tggatctggc cggacttctc gttgccttcc tcgttgatcg ggatgcggat
     5221 cttgccgcag gggctggtca tcgccttgct cttgacgccc gtcgcctggc cctcgatgtc
     5281 gaagtagcgc acctcgcgga agttgaacag gcgttcgtag aagcccgccc attcctccat
     5341 gcggccgcgg tgcacgttgt gcgtgaggtg gtcgatgtag gtcaggccga tgcccacggg
     5401 gttgagttcc gcgcccgagt ccttcgagat gggctcgaaa tccacgtcga agaagccgat
     5461 gttgccgatg tcgccttcct tcgcgccgtt cttgccgcgc cagcggtcga ttaaatagat
     5521 gatcgagtcg ccgatgccct tgatggcggg gatgttcaac tcgccggggc cggcctggcc
     5581 cgcatagccc caggcgccca acgagaccgc gcgcccgtac gcggccttgg catccttcac
     5641 ccggaaggcg atggcacaca cgctgggccc gtgcagccgt gcaaaacgct gcgcgaagga
     5701 gtcgggctcg gcgttgagga tgaagttgat gccgccctgg cggtacagca gcacgttctt
     5761 gtggcggtgc ctggcgatgg gcttgaagcc catgcgctcg aacaattcgc ccatggcttt
     5821 gggatctggc gcggcgtatt cgatgaattc gaagccgtcg gtgcccatgg ggttctccca
     5881 ggcgtcggtc ctggcggggg cttgttcagg cagtcttgcg ttcatggggt gtctccagcg
     5941 gcgggcagca tcttgcttcg cactgtggaa ccaatgatgg tcaattttcc gccgaaggag
     6001 cgccccgcac gcttctcgct tgcaagaaaa ctgcgttctt ggagaaagtt acgccgcggc
     6061 gctcagcgct gagcttggcg agccgcgcaa ggcacgcagc gccagggccg catcgatgcg
     6121 cagctgctcg tgcggcgcga agagttgttc gcgcagctgc tcggattggc attcgagccg
     6181 taaaattgcg ttgccatgga gagccacgca ctcgacaagc tcgacaaagc catcctgcgc
     6241 tgcctgcagc agaatggccg cgagacctac gacgtgatcg gtgcgcaggt cggcctctcg
     6301 tccagcgccg tgctgcggcg ggtcaagcgt ctggaggaag ccggcgtgat cgaccggtac
     6361 gtcgccctcg tcaagccgga ggcggtgggg ctcgggctga ccgcgtacct caacgtgcgg
     6421 ctggaaaagc ataccgagag ccacaagcgc aacccgatgg atctgtttcg cgccagcgtg
     6481 caaacctggc ccgaggtggt cgaatgtgct gcgctgacgg gcgagatgga ctacctgctg
     6541 cgggtcgtgg tgcaggacat ggcccactac aggcgcttca tcatggacac gctgctcaag
     6601 cacccgagcg tgcaagattg caagaccagc ttcgtgctcg accgcgtcaa ggcgacgacg
     6661 gccatgccgt tgtaggataa aaggacaaca acctgctcgt caataccgca aactcgaact
     6721 caggtgttga ttttcggcga cctccgcaag aactaggtag aactacctta ccgtagcggg
     6781 taacccacat tccggcttgg gtttgcccgt ggaagccctt aaattgaaga catgactgaa
     6841 agcgaagaac tcaccaagac cccggtgaac gttcctatcc gttccatcgg gcccagccac
     6901 cgcgatcgca ttaccgctca tctgatggcg ctcgacgaac acgaccgcta cctgcgtttc
     6961 ggttacgccg caaccgatga gcaagtccag cgctacgttg caagcctgga tttcgagcgc
     7021 gatgaaattt ttggcgttta caaccgcagg ctcgagttga ttgcgatggc gcacctggcg
     7081 ttctctgtcg acccggccct cagggcatgc gctgaattcg gcgtgtccgt ggtgaaaaaa
     7141 gcgcgcggcc gcggcttggg cgcccgcctg ttcgaccgcg ccgtcatgca tgcgcgcaac
     7201 gagggtgtgg agatgctgtt catccacgcg ctgagcgaaa acacggcgat gctcaagatc
     7261 gcccgcaatt ccggcgccac gctcgagcgc gcaggcagtg aaacggacgc ctacctgcgc
     7321 ctgccgcccg cgacactgga cagtcggatg agtgaaatcc tcgcagagca actcgcccag
     7381 accgactatc gcctgaaagc gcaggccaag agcttctggg acttcatttc cggtgttcag
     7441 gaagtgcgcc agggggtgcg cgacgcccgg cacaagtccg gcggctgagc tggaaagagg
     7501 cggctgagcc gggggcaagc ccctcgggac aatgccgcca caagtggcaa aacgtggtcg
     7561 ccacattctt ccgctatcct attggtcccc caactaccgc acgtcctgca acccaaggct
     7621 gtgtccgacc cgcaccccgc gcgcgcttcc gccgagaagg aagacaggcg cactttcctg
     7681 caaaaaatcg ccgagttcat ccatcccgga ccggactcca aggccgaact gatagaaacg
     7741 ctggcggacg ccgaggacaa cgacatcatc ggcgccgaat cccgggtgat gctcgaggga
     7801 gtcatccgca tggcggagct gaccgcgggc gacgtgatgg tggcggcccc gcgcatggac
     7861 ctgctcaaca tcgactcgcc ctacgacgag atcctggaaa tcgtgatcga tacggcgcac
     7921 tcgcgctttc cggtctacga aggcgaccgc gaaaacatca tcggcatcct gctggcgaag
     7981 gatctgctga agctgcagcg cgcgcccgag ctgaacatcc gggcgctgct gcggcccgcc
     8041 gtgttcgtgc cggaaaccaa gggcctgaac gacctgctgc gcgaattccg cggcaaccgc
     8101 aaccacctgg cgatcgtgat cgatgagttc ggccgcgtgg ccggcctgat caccatcgag
     8161 gacgtgctcg agcagatcgt cggcgagatc gaggacgagt tcgacatcga cgaggacgag
     8221 ggcgacatct tcggcctggc cgaccgcacc taccgtgtca gcggcgacac cgccgtggag
     8281 cgggtcggcg aagccttcgg cgtcaccctg gagcccaccg acgccgacca ggaattcgac
     8341 accatcggcg ggctgatcgc gcacgaaatg ggccatgtgc ccaagcgcgg cgagcactac
     8401 acgctgggcg ggctgaattt cgtggtcatg cacaccaagg gcggcgcggt gcgctggttc
     8461 aaggtgtcgc cgaccaagga cgacgaccat acgggataaa tgaactcccc cccgaagcgg
     8521 cctgcggccg atgccccctc gggcttcgcc ctctcccccc aaggggggcg ccaccagcgg
     8581 cccggcaaag ccggttccgc ggtggccccc gattgatgtt tctcctggca ctagcgctgc
     8641 tggccgggct gctgcaggcc gcgtcgatcg ctgcgccgtg gagcggccag ccggcgtggt
     8701 ggctgcagct gctgtcgctg gcggtgctgg cctggctggc gcagcaggcg ccctcgtggc
     8761 ggcgcgcagg cttgctaggc tggaccttcg cgacggcgtg gctggcgggc accttctggt
     8821 ggctgttcat ctccatgcat gtctacggcg ggctgcccgc gccgctggcc gccttcgcgg
     8881 tgctggcgct ggctgcgttc ctggcgacct actacggcgc cgcgtgcgcc gttttcgcgc
     8941 tggtccgcgg gagtactcgc ctgccggggg ctctggcgtt cgcggcgttg tggctgctgg
     9001 ccgaactgct gcgcgtcacc tggttcaccg gctttccctg gggtgccgga ggctacgcgc
     9061 atgtggacgg gcccctgtcc tttctggccc cccaggtggg cgtgcacggg at
//
LOCUS       DASWIY010000076         6090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3371897, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000076 DASWIY010000000
VERSION     DASWIY010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3371897"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..3332
                     /locus_tag="VGA59_02910"
     CDS             81..3332
                     /locus_tag="VGA59_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9718654.1"
                     /translation="MSLAQRRTPNAQPPKWFTRISLRNPVFATMVMLAIVVLGLFSLQ
                     RLKVDQFPNIDFPVVVVTAEYPGASPEIVESEVTKKIEEGVNSIAGINALTSRSYENQ
                     SVVIIEFQLHIDGRKAAEDVREKMAAIRPTFRTEVKEPRVLRFDPSARAIWSVAVLPD
                     AAKGKAMNAVELTNWADQVLKKRLENVRGVGSVTLVGGTKREINVYLHPAAMEALGIT
                     ADQVVAAVRNENQDLPVGAIRSLAQERVVQIDARMERPEDFGRIIVARKGSGSAGQAG
                     AGAPVRVDQVARISDGAQEIDSLALYNGGRTLLLTVQKSQDENTIEVVDGLKKTVAEM
                     AAQLPAGVRLEQITDNSRPIRVSVENVRRTLIEGGLLTVLIVFLFLNSWRSTVITGLT
                     LPISIIGTFLFMNMFGFTINMITLMALSLCVGLLIDDAIVVRENIVRHVQMGKSPYQG
                     ALDGTQEIGLAVLATTFSIVAVFLPIGFMGGIIGKFFHEFGVTIVAAVLISMFVSFTL
                     DPMLSSVWHDPEIEAHGKPKANPNLYDKTIGRVTGWFDHATDWAADAYQAILRWSLVH
                     KLATVLLAVAVFATSVVMVPLLGTEFVPKADFSETSLNFYTPVGSSLEATEAKARQVE
                     AIMREFPEVKYTLSTINTGNAQGKIYASIYVRLVDRKERTRSADQMSGVLRERLKQVP
                     GATVTHAGLLDPVGGNKQIEFSLQGQDLRELERLSRLVIDKVRDVPGLVDLDSSVKPD
                     KPVIDIEVKRDAASDLGLSVAQIAGSLRTLVAGQTVGNWRAPDDQTYDVNVRLSPDAR
                     NAPRDLERLPFATGSNADGSTRIVRLNQVATVKEATGPNQINRRDLTREVAINANTFN
                     RSAGEVSNDIRARLDTIAFPPGYRYSFSGSTKNMAESFGYAVSALAMGVIFIYMILAS
                     QFKSFLQPMALMTSLPLTLIGVVLALLMFGSTLSIFSIIGIVMLMGLVTKNAILLVDF
                     AIRMREQGQSRTDALLHAARVRLRPILMTTLAMIFGMVPLAFALTEGSEQRAPMGQAV
                     IGGVITSSLLTLVVVPVVYCYLDDLANWLRRRPRASAGGGAQAVPASKIDHLP"
     gene            3357..4046
                     /locus_tag="VGA59_02915"
     CDS             3357..4046
                     /locus_tag="VGA59_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX9718655.1"
                     /translation="MNAPANLEQARYKMIEQQIRPWNVLDSQVLELLSVVRREDFAPL
                     AHKALAFVDMKIPLLAPADEAMQHGHCMLEPKVEARLLQDLHVGPKDKVLEVGAGSGY
                     MAALLAHRAQRVITLEINPQLAQMARSNLQGAGIGNVEVRQADGVKGLPGEAPFDVIV
                     LSGSVAEVPHALLEQLKIGGRLAAIVGFEPVMRATFITRTGDASYATTQPWDTVAPRL
                     ANFPEPSKFHF"
     gene            4109..4453
                     /locus_tag="VGA59_02920"
     CDS             4109..4453
                     /locus_tag="VGA59_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9718656.1"
                     /translation="MIDQVRPADLAAWCQDQAAGPGPAVVLDVREPWELQTASVKPDG
                     FEVVAIPMNEVPARLGELDPARPLACLCHHGTRSQRVASFLAQHGFGAVANIAGGIEA
                     WSRERDPAVPRY"
     gene            4481..5815
                     /locus_tag="VGA59_02925"
     CDS             4481..5815
                     /locus_tag="VGA59_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX9718657.1"
                     /translation="MKSLRPYMRFLPLSVALGAALALPARAQNLLELYESARAYDATW
                     QSAKAQYDANLFRAEQARAGILPSANLGAGVTRSNFENTVPVTDRSFTTQSATISAAQ
                     PLYRPANFATWEQGKRQVELAQAQLSTASQDLIIRVSQAYFDVLAAQDTLTFVRTQKA
                     AVDEQLASAKRNFEVGTTTITDTREAQARFDLVVAQEIAAENDLRVKKLALDALVGKP
                     DVQPQPLAVPVIVPAPQPPDVNAWVQQGEENSPAIQQAKTAVEIAQLETEKAKAGHKP
                     TLDLTASYNITRNPSGSTLAAVGNRTNSASIGVSFNLPLFAGFATQNRIRETLSLEEK
                     AQADLESTRRTVAQATRTAFFGVVSGQGQVKALEAAEASSQSALEANRLGYQVGVRIN
                     IDVLNSQSQLFQTKRDLAQARYNVLLGQLRLRQANGTLTPEDLTRLNALVVK"
     gene            5905..>6090
                     /locus_tag="VGA59_02930"
     CDS             5905..>6090
                     /locus_tag="VGA59_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015472933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX9718658.1"
                     /translation="MTITSISSREFNQDASGAKKAARRGPVFITDRGRPAHVLLSIED
                     YQKLAGGGMTLLDAIAQP"
BASE COUNT         1103 a         2072 c         1951 g          964 t
ORIGIN      
        1 cggccctcac cccagccctc tcccgcaagc gggagaggga gcaactaccc gggctccggc
       61 caaatccccc agcccgtgac ctgtcgctcg ctcaacgccg gacgcccaac gctcaacctc
      121 ccaagtggtt cacccgcatc agcctgagga acccggtctt cgcgaccatg gtgatgctcg
      181 ccatcgtcgt gctggggctg ttttccctgc agcgcctgaa ggtcgaccag tttcccaaca
      241 tcgacttccc ggtggtcgtg gtgacggccg agtatcccgg cgcatcgccc gagatcgtcg
      301 aaagcgaagt caccaagaag atcgaggaag gcgtcaactc gatcgcgggc atcaacgccc
      361 tcacctcgcg cagctacgaa aaccagtcgg tcgtcatcat cgagttccag ctccacatcg
      421 acgggcgcaa ggcggccgag gacgtgcgcg agaagatggc ggctatccgt ccgaccttcc
      481 gcaccgaggt gaaggagccg cgggtcctgc ggttcgaccc gtcggcccgc gcgatctggt
      541 cggtggccgt gctgcccgat gccgcaaaag gcaaggcgat gaacgccgtg gagctcacca
      601 actgggccga ccaggtgctc aagaaacggc tggagaacgt gcgcggcgtg ggctcggtca
      661 cgctggtggg cggcaccaag cgcgaaatca acgtctacct ccacccggcc gcgatggaag
      721 cgctgggcat cacggccgac caggttgtcg cggccgtgcg caacgagaac caggacttgc
      781 cggtcggcgc gatccgctcg ctcgcgcagg agcgcgtggt gcagatcgac gcgcgcatgg
      841 agcgtccgga ggatttcgga cggatcatcg tcgcgcgcaa gggatcagga agcgccggtc
      901 aggccggcgc gggcgcgccg gtgcgcgtgg accaggtggc ccgcatctcc gacggcgcgc
      961 aggagatcga cagcctggct ctgtacaacg gcgggcgcac gctgctgctc acggtgcaga
     1021 agtcgcagga cgagaacacc atcgaggttg tggacggcct gaaaaagacc gtggcggaga
     1081 tggcggcgca gctgccggcc ggcgttcggc tggagcagat cacggacaac tcgcgcccga
     1141 tccgcgtttc cgtggaaaac gtccgccgca cgctgatcga gggcgggttg ctgacggtgc
     1201 tgatcgtgtt cctgttcctc aattcctggc gctcgacggt gatcaccggc ctcacgctgc
     1261 cgatctcgat catcggcact ttcctgttca tgaacatgtt cggcttcacc atcaacatga
     1321 tcacgctgat ggcgctgtcg ctgtgcgtgg gcctgctgat cgacgacgcg atcgtggtgc
     1381 gggagaacat cgtgcgccac gtgcagatgg gcaagagccc ctaccagggg gcgctggacg
     1441 gcacgcagga aatcggcctg gcggtgctgg cgaccacatt ctccatcgtc gcggtgttcc
     1501 tgccgatcgg cttcatgggc ggcatcatcg gcaagttctt ccacgagttc ggcgtgacca
     1561 tcgtcgcggc ggtgctgatc tcgatgttcg tgagcttcac gctcgatccg atgctgtcgt
     1621 cggtctggca cgacccggaa atcgaggccc atggcaaacc caaggccaac ccgaaccttt
     1681 acgacaagac catcggccgt gtgaccggct ggttcgacca cgccacggat tgggcggccg
     1741 acgcctacca ggccatcctg cgctggtcgc tggtgcacaa gctggccacc gtgctgctcg
     1801 ccgtcgcggt gttcgcgacg agcgtggtca tggtgccgct gctgggcacg gagttcgtgc
     1861 ccaaggcgga cttctccgaa acctcgctca acttctacac gccggtcggc tcctcgctgg
     1921 aagccaccga agccaaggcc cggcaggtcg aggcgatcat gcgcgagttt cccgaggtga
     1981 agtacacgct ctcgaccatc aacaccggca acgcgcaggg caagatctac gcatcgatct
     2041 acgtgcgcct ggtcgaccgc aaggagcgca cgcgcagcgc cgaccagatg tccggcgtgc
     2101 tgcgcgagcg gctcaagcag gtgccgggcg ccaccgtcac ccacgccggg ttgctcgacc
     2161 cggtgggcgg caacaagcag atcgagtttt cattgcaggg gcaggacctg agggagctgg
     2221 agcgcctgtc ccgcctcgtc atcgacaagg tccgcgacgt ccccggcctg gtggacctgg
     2281 attccagcgt caagcccgac aagccggtga tcgacatcga ggtcaagcgc gatgccgcgt
     2341 ccgacctggg cctgtcggtg gcacagatcg ccggctcgct gcgcacgctg gtggccggcc
     2401 agacggtggg caactggcgc gcgcccgacg accagaccta cgacgtgaac gtccgcctgt
     2461 cgcccgacgc gcgcaacgcc ccgcgggacc tggagcgctt gcccttcgcc accggcagca
     2521 atgccgacgg ctccacgcgc atcgtgcggc tgaaccaggt tgccaccgtg aaggaagcga
     2581 cggggcccaa ccagatcaac cggcgcgacc tcacgcgcga ggtcgccatc aacgccaaca
     2641 ccttcaaccg ctcggccggc gaagtgtcca acgacattcg cgcgcgcctg gacaccatcg
     2701 ccttcccgcc cggctaccgc tattcgttca gcggctccac caagaacatg gccgaatcgt
     2761 tcggctacgc ggtgtcggcc ctggcgatgg gcgtgatctt catctacatg atcctggcca
     2821 gccagttcaa gagcttcctg cagcccatgg cgctgatgac atccctgccc ctcacgctga
     2881 tcggcgtggt gctggccctg ctgatgttcg gctccacgct gtcgatcttt tccatcatcg
     2941 gcatcgtcat gctgatgggc ctggtcacca agaacgctat cctgctggtg gacttcgcga
     3001 tccgcatgcg cgagcagggc cagtcccgca ccgatgcgct gctgcatgcc gcccgggtgc
     3061 ggctgcgccc catcctgatg accacgctgg ccatgatttt cggcatggtg ccgctggcct
     3121 tcgcgctgac ggagggatcg gagcagcgcg cgccgatggg ccaggccgtg atcggcggcg
     3181 tgatcacctc ttccctgctg acactggtgg tggtgccggt cgtctactgt tacctggacg
     3241 acctggcgaa ctggctgcgg cgccgcccgc gtgcttccgc cggtggcggc gcccaggcgg
     3301 tgcccgcctc taaaatcgac catttgccct gatatcaatc ctacggagaa accgcaatga
     3361 acgctcccgc caacctcgaa caagcccggt acaagatgat cgagcagcag atccgtccct
     3421 ggaacgtgct cgactcccag gtcctggagc tgctgtccgt ggtccgccgt gaagacttcg
     3481 cgcccctggc gcacaaggcc ctggctttcg tcgacatgaa gattccgctg ctggcgccgg
     3541 ccgacgaggc gatgcagcat ggccattgca tgctggagcc caaggtcgag gcgcgcctgc
     3601 tgcaggacct gcacgtcggg ccgaaagaca aggtgctgga agtcggcgcc ggctccggct
     3661 acatggcggc gctcctggcc catcgcgccc agcgcgtcat cacgctggag atcaacccgc
     3721 aactggccca gatggcccgt tcgaacctgc aaggtgcggg catcggcaat gtcgaagtgc
     3781 gccaggccga cggcgtcaag ggcttgccag gggaagcccc tttcgacgtg atcgtgctga
     3841 gcggctcggt cgccgaggtg ccgcatgccc ttctcgaaca gctcaagatc ggtggccggc
     3901 tggccgccat cgtcggcttc gagccggtga tgcgcgcgac gttcatcacg cgcaccggcg
     3961 atgcttccta cgcgacgacg cagccgtggg acacggtcgc accgcggctg gccaatttcc
     4021 ccgagccgtc caagttccac ttctgatact ttgcgggaca gatcaagaat tgcgcagcaa
     4081 tattgctctg tcctcaactg attaagcgat gatcgaccag gtgcgcccag ccgatctcgc
     4141 cgcatggtgc caggaccagg cggcgggccc ggggccggcc gtcgtgctgg acgtgcgcga
     4201 gccctgggag cttcagaccg ccagcgtcaa gcccgatggc ttcgaggtcg tggcgatccc
     4261 gatgaacgag gtccccgcgc ggctgggcga gctcgacccg gcgcggcccc tcgcgtgcct
     4321 gtgccatcac ggcacgcgca gccagcgcgt cgcctcgttt ctcgcgcagc atggtttcgg
     4381 ggcagtggcc aacatcgcgg gcggcataga ggcctggtcg cgcgaacggg acccggccgt
     4441 gccccgctat tgagaacaac caacgaagaa aaggaccacc atgaaatcgt tgcggccgta
     4501 tatgcggttc ctgcccctgt cggtggcgct cggcgcggca ttggccctgc ccgcgcgcgc
     4561 acagaacctg ctcgaactgt acgagtcggc gcgcgcctat gacgccacct ggcagtcggc
     4621 caaggcccag tacgacgcca acctgttccg cgccgagcag gcccgggccg gcatcctgcc
     4681 gtcggccaac ctgggagcgg gcgtcacgcg ttcgaatttc gagaacaccg tcccggtcac
     4741 cgaccgcagc ttcacgaccc agagcgccac catcagcgcc gcgcagccgc tgtaccggcc
     4801 ggcgaatttc gccacctggg agcagggcaa gcgccaggtc gagctcgcgc aagcccagct
     4861 gtcgacggcc agccaggacc tgatcatccg cgtaagccag gcctatttcg atgtgctggc
     4921 ggcgcaggac acgctcactt tcgtgcggac gcaaaaggcc gccgttgacg aacagctggc
     4981 gtccgccaag cgcaatttcg aggtcggcac aacgaccatc accgacacgc gggaggcgca
     5041 ggcgcgcttc gacctggtgg tcgcccagga aatcgcggcc gagaacgacc tgcgcgtgaa
     5101 gaagctcgca ctcgatgcgc tggtcggcaa gcccgacgtg caaccgcagc cgctggcggt
     5161 gcccgtcatc gttccggcgc cgcagccgcc cgacgtgaat gcgtgggtcc agcagggcga
     5221 agagaacagc ccggcgatcc agcaggcgaa aaccgcggtg gagatcgcgc agctcgaaac
     5281 cgaaaaggca aaagccggcc acaagccgac cctcgacctc acggccagct acaacatcac
     5341 gcgcaacccc agcggaagca ccctcgccgc cgtcggcaat cgcaccaact cggcaagcat
     5401 cggcgtttcc ttcaacctgc cgctgttcgc cggcttcgcg acccaaaacc gcatccgcga
     5461 aaccttgtcg ctggaagaaa aggcccaggc cgacctggaa agcacgcgcc gcaccgtggc
     5521 gcaggcgacc cgcaccgcct tcttcggcgt cgtctcgggc cagggccagg tgaaagcact
     5581 ggaggcagcg gaagcgtcca gccaaagcgc gctggaagcc aaccgcctgg gctaccaggt
     5641 aggcgtgcgc atcaacatcg acgtgctcaa ttcgcaaagc cagctgttcc agaccaagcg
     5701 cgacctggcg caggcccgct acaacgtgct gctgggccag ctcaggctgc ggcaagccaa
     5761 cggcacgctg acgccggaag atttgacgcg gttgaatgcg ctggtggtga agtagccttc
     5821 tttggacgag cgggggttga caagacagtc gacaggcgaa aagtagtaac atatctacat
     5881 ttcaaagtta gatagggcct cgccgtgacg atcacctcca tttccagccg agagttcaac
     5941 caggatgcga gcggggccaa aaaggccgca cggcgcggcc ccgtgttcat caccgatcgc
     6001 ggccgccccg cgcatgtgct gctgtcgatc gaggactacc agaaactggc gggcggcggc
     6061 atgaccttgc tggatgccat cgcccagccg
//
LOCUS       DASWIY010000077         9054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3010239, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000077 DASWIY010000000
VERSION     DASWIY010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9054
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3010239"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="VGA59_02935"
     CDS             complement(<1..1545)
                     /locus_tag="VGA59_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9718659.1"
                     /translation="MHLARRRIYPRRRIRVVSGLSQLINPGPHQTLASELRRAAHDSA
                     DRPFLRMRHREWTFKEFDGLTDACAAGFSHLAITLQENVSLLLPNCIEFAIAWFGLTK
                     IGAVAAPVNTSFRGAVLASAFNLVDSRVLVVHQDLLPALLEILAELPNIREVVVVGEA
                     LVELEKSGVRWTRWQDLAEDSTASYEPREVPFSALTLLLYTSGTTGRSKAAMISHRYV
                     LEQARLTVQGLKLRQDDVLYCPYPMFHLDSAVMTIAPALVLRCVAAIGERFSVSKYWD
                     EVRALGATVFDFMGATLTMLWKQPPSPKDRDHRARLGWGVPMPAWAEAFEERFGCQLV
                     ELYGSTEVGSMIFNPPDQPRRVGSCGRPIGPFDVALLDEDGFEVGPGVPGELVIRPKA
                     PSMIMDGYYGMPEETLQAFKNLWFHTGDVLKRDVDGYLYFVGRCKDMIRRRGENISAA
                     EVEMVIEEHPDILECAVYGVPSEMTEEEVMVCAVTREGRSLSPQDLTDYCAQRMASFM
                     VPRFVRF"
     gene            complement(1650..2018)
                     /locus_tag="VGA59_02940"
     CDS             complement(1650..2018)
                     /locus_tag="VGA59_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX9718660.1"
                     /translation="MTKAGTQILSLEGLRFDANLGILDHEKSGPQPILVDAQLNLGTQ
                     PLLPRDDDIMHVLDYRKVRRIIIDECTAEHVNLLESLIGKLANRLMQLPGVIGVRVRI
                     VKLEIFDDCEVAIRVEAGLW"
     gene            complement(2099..2890)
                     /locus_tag="VGA59_02945"
     CDS             complement(2099..2890)
                     /locus_tag="VGA59_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9718661.1"
                     /translation="MSDSPRLRTALVTGSATRLGREVALALAAAGWQVAVHFRASAAQ
                     AAQTVADCLRLAPTGVQVDSFQADLDDESQARALLPRVVQRFGAVDAVVNNASMFEHD
                     SIGSFGYDALLAHVRTNTGAPILLAHALHQHVCARTGPARAADAVVVNMLDQKLWNQN
                     PDFLSYTLSKGALEAATTMLAIALAPQLRVVGVAPGLTITSQFISQEKFDALHQLSPL
                     GRSSTPQDVVAAVKFALDNSSITGTTLLVDGGQHLMKFDRDFSLM"
     gene            2900..4009
                     /locus_tag="VGA59_02950"
     CDS             2900..4009
                     /locus_tag="VGA59_02950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9718662.1"
                     /translation="MTEVTTEATSLTRALRALVDEAIRRAGGWLPFDRFMALALYAPG
                     LGYYAHGSRKFGQLPSSGSDFVTAPEMTPLFGQALAAQLADALDATGTDEVWEFGAGS
                     GALAAQLLRALGKRVRRYTIVDLSGSLRARQREALGGFAGTVEWAGELPNALRGVVIG
                     NEVLDAMPVKLLARVKGVWHERGVTADGPAGAFDWADRPTELRPPVDIAGPHDYLTEI
                     HRQAEAFMRTLADRLAQGAAFFIDYGFTEAEYYHPQRHMGTLMCHRGHLADSDPLPDL
                     GLKDITAHVNFSGLALAAQEAGLPTLGYTTQARFLMNCGLLPLMEQAPLAERVAAHRL
                     LAEHEMGELFKVIGFHRGAFWDAVGFRDGDRTHTL"
     gene            4051..4245
                     /locus_tag="VGA59_02955"
     CDS             4051..4245
                     /locus_tag="VGA59_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX9718663.1"
                     /translation="MIRWIIVIFLALILISWFSPLLQRMGFGKLPGDLHFKAFGREWF
                     IPLTTTILLSLVAGAISKFV"
     gene            4256..5179
                     /locus_tag="VGA59_02960"
     CDS             4256..5179
                     /locus_tag="VGA59_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX9718664.1"
                     /translation="MKACIDIGGTKVAVSLNSGAGLELVARRSEPTAKTGANDALARQ
                     VIRMVDESCAELGIAPDRVARAGVSSCGPFVLRGGLVEVAAPNICGGIAGPARGLPND
                     WETALLEAPLRRRFGQVRVENDCIAALEAERRWGALQAFDHCAYVTWSTGIGVGLCVD
                     ARVLRGKNGNAGHAGHMFVSDNDDALCGCGNIGDVEALVAGNSIERRFGADAATLLQR
                     AREGDAQAEAIVDELCRVMGRALYNLIATLDLQRISLGGSVFWNNSGYLLPRLQAQVA
                     GKFPALTAGCELVPAGLADRVGDYAALALVV"
     gene            complement(5365..5772)
                     /locus_tag="VGA59_02965"
     CDS             complement(5365..5772)
                     /locus_tag="VGA59_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718665.1"
                     /translation="MDFLRGNHQNRRPADGIRRVGFRKWYERELLSSHAYMILAILSF
                     VAMAASFEAFNGGTMSEKLMNVLFVIVSAGIGLWALRRYLFLLMRAEEVANQARCGDC
                     GEYGRFRVVAEDRSHQETQVRCSKCEHLWTICT"
     gene            complement(5811..7058)
                     /locus_tag="VGA59_02970"
     CDS             complement(5811..7058)
                     /locus_tag="VGA59_02970"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEX9718666.1"
                     /translation="MQTYMVGGAVRDALLGLPVNDHDWVVVGATPHEMIADGYLPVGR
                     DFPVFLHPRTREEYALARTERKTARGYHGFTFHAEPNVTLEQDLARRDLTINAMAQDE
                     NGRLIDPFGGRADLARRVLRHVTTAFREDPVRILRVARFAARFADFVVAPQTVALIRD
                     MVQAGEVDALVAERVWQELARGLMEHKPSRMFEVLRESGALARLLPEVDRLWGVPQRA
                     EYHPEVDTGVHLMMVLDMSARLKAPIAVRFACLTHDLGKGTTPAEILPKHIGHEERGA
                     RLLSKLCERLRVPTECRELADVVAREHGNIHRSGEFGAAALVRLLERCDAFRKPQRFD
                     LVLLACECDARGRLGLEEKPFAARQRLLAALAAAQAVATHEIAAQAQQQEIEGPAIGE
                     MIHKARVQAVAKQVLGQRDSLLP"
     gene            complement(7120..7881)
                     /locus_tag="VGA59_02975"
     CDS             complement(7120..7881)
                     /locus_tag="VGA59_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9718667.1"
                     /translation="MLKLYIGNKNYSSWSMRSWVLLKQAGIQFEEVMVRFDSLDADSA
                     FKRTLKDLSPTGRVPVLVDDGFVVWDTLAIAEYVAEKFPDRQLWPRDAKARARARSIC
                     AEMHSGFTGLRGHCPMNVEASLPQIGALAWRDRPAVRADVQRIVSMWTALLEEHKGPM
                     SNAGPPQGASAPSGGRETRSSVGGQFLFGEFSVADAYFAPICVRLKNYALPVPGHITD
                     YIRRVCALPGVKAWIEEALAEQDFLDFEEPYRTGR"
     gene            complement(7979..8491)
                     /locus_tag="VGA59_02980"
     CDS             complement(7979..8491)
                     /locus_tag="VGA59_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX9718668.1"
                     /translation="MAWWHAVIETIASEFSDLPDVPQFTRLVLRLLLAALLGGLLGLQ
                     RERHGKEAGVRTHMLVALGAAMFVLVPQQAGMEAADLSRVMQGVIAGIGFLCAGTILK
                     SGDDQVRGLTTAAGIWLTAAIGMAAGVGRETTAVLSTMLALAILALEGPIRRLMRGRG
                     AQGAEADREH"
     gene            complement(8501..>9054)
                     /locus_tag="VGA59_02985"
     CDS             complement(8501..>9054)
                     /locus_tag="VGA59_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9718669.1"
                     /translation="EQRFPMPFRESVVKRSQEIGLDPAYVYGLIRQESRFIMDARSNV
                     GASGLMQVMPATARWTARKIGLANFAPHQLSDRETNIAIGTGYLKLVLDDFDGSMPLA
                     AAAYNAGPGRPRSWRNGAVIEAAAWAENVPFGETRDYVKKVLANTTIYAAILSGQPQS
                     LKARLGMVGPRDASLPEPNGELP"
BASE COUNT         1529 a         2998 c         3008 g         1519 t
ORIGIN      
        1 gaatctgacg aatcgaggca ccatgaagct cgccatccgt tgggcgcagt agtccgtcag
       61 atcttgcggc gaaagcgagc ggccctcgcg cgtgacggcg cacaccatca cttcttcttc
      121 tgtcatttcg ctgggcacgc cgtagacagc gcactcgaga atgtcagggt gttcctcgat
      181 caccatttcg acttctgccg cggagatgtt ctctccgcgg cggcgaatca tgtccttgca
      241 gcgtccgacg aagtacaggt agccatccac atcccttttc agaacgtcac ccgtgtggaa
      301 ccagaggttc ttgaacgctt gcagcgtctc ctcgggcatt ccgtagtaac cgtccatgat
      361 catggacggc gctttgggcc ggatcaccag ctcgccaggt acgcccggac cgacttcgaa
      421 gccgtcctca tcaaggagcg ccacgtcgaa aggaccgatg ggcctcccgc aggagccaac
      481 gcggcgaggc tggtccggcg gattgaaaat catgctgccg acttccgtcg agccgtaaag
      541 ctccaccagc tggcagccga atcgctcttc gaacgcctcg gcccaggccg gcatgggcac
      601 gccccacccg agccgggcgc gatggtctct gtccttgggc gagggaggct gcttccacaa
      661 catcgtgagc gttgcaccca tgaagtcgaa cacggtcgcg cccaaagcgc ggacctcatc
      721 ccagtatttc gaaacgctga agcgctcacc aatcgcggcc acgcaacgca gaaccagcgc
      781 cggggcgatc gtcatcacgg ccgaatccag gtggaacatc ggataggggc agtacagaac
      841 gtcgtcctgc cgcaacttca gcccttgcac ggtcaggcgc gcctgctcga gaacgtaacg
      901 atgggagatc attgccgcct tggagcggcc ggtggtaccc gaggtataga gcagtagggt
      961 caacgcggag aacggaactt cgcggggctc gtatgaagcc gtgctgtctt cggcgagatc
     1021 ctgccatcgt gtccagcgga cgccggactt ctcgagttcg accagtgctt caccgacaac
     1081 caccacttcg cggatgttgg gaagttccgc caggatttcc agaagcgcag gcaagagatc
     1141 ctgatgaaca accaagacgc gactgtccac cagattgaag gcgctggcca gcactgcgcc
     1201 gcggaacgag gtgttcaccg gggcagccac tgcgccgatc ttcgttagac cgaaccacgc
     1261 gattgcaaat tcgatgcaat tcggaagcag caggctgacg ttctcctgga gcgtgatcgc
     1321 caggtgcgaa aaaccggcag cgcacgcgtc cgtgagtcca tcgaactcct tgaaggtcca
     1381 ttcgcggtgc cgcattctca gaaaaggcct gtcagccgaa tcatgcgctg cacgcctgag
     1441 ttcgcttgcg agggtctgat gagggccagg attgatcaat tgcgatagtc cggaaacaac
     1501 tcgaatacgt ctgcggggat aaatcctacg tcgtgcaagg tgcatgccaa ggcgaagcgc
     1561 cgctggcagt gatgggcatc aaagagggat ggacgcgtgc gtgcgggggg aagtgtggcc
     1621 acatagcgtg cgttctcgac agcaagccgc taccagagcc cggcttccac ccggatcgcg
     1681 acttcgcagt cgtcgaagat ttccagcttg acgatgcgca cccgcacgcc gatcacgccg
     1741 ggcagctgca tgagccggtt cgccagtttg ccgatcaggc tttcaagcag gttcacgtgc
     1801 tcggccgtgc actcgtcgat gatgatccgg cgtaccttgc gatagtccag cacatgcatg
     1861 atgtcgtcgt cgcgcggcag cagcggctgc gtgcccaggt tcagctgggc atcgaccagg
     1921 atcggctgcg ggcccgattt ttcgtggtcc aggatgccca ggttagcgtc gaaacgcagc
     1981 ccttcgagcg agaggatctg ggtgccggct tttgtcatga gcgttgtgtc gttagcattc
     2041 tgatctgtta gggtcagagc acaattgctg cgcaattcgt gatctgaccc gcaaggcctc
     2101 acattagcga aaaatcgcgg tcgaacttca tcaggtgctg gccgccatcc accaacaggg
     2161 tggtgccggt gatcgacgaa ttgtccagcg cgaatttcac ggccgccacg acgtcctgcg
     2221 gcgtcgacga gcgccccagc ggcgagagct ggtgcaacgc atcgaatttt tcctgcgaaa
     2281 tgaattggct ggtgatcgtc agcccgggcg cgacgcccac gacgcgcagc tgcggggcca
     2341 gcgcgatggc cagcatggtg gtggccgctt ccagcgcgcc cttggacagc gtatagctca
     2401 ggaagtcggg attctggttc cacagcttct ggtccagcat gttcaccacg acggcgtcgg
     2461 cggcgcgggc cggcccggtc ctggcacaga catgctggtg caatgcatgg gccagcagga
     2521 tgggcgcgcc ggtattggtc cgcacgtggg ccagcaaggc gtcgtagccg aaacttccga
     2581 tactgtcgtg ttcgaacatc gaagcgttat tgacgacagc atcgacggcg ccgaagcgct
     2641 ggacgacgcg gggcagcaga gcgcgcgcct gggactcgtc gtccaggtcg gcctggaagc
     2701 tgtcgacttg cacgccggtg ggcgcgagcc gcaggcagtc ggccacggtc tgcgccgcct
     2761 gggctgccga tgcgcggaaa tgcacggcca cctgccagcc ggccgccgca agcgccagcg
     2821 cgacctcacg gccgagccgt gtggcggagc cggtgaccag cgcggtgcgc aaacggggcg
     2881 aatcgctcat tcggggacaa tgacggaagt gacgactgaa gcaacgagtt taacgagggc
     2941 cctgcgggcc ctggtggacg aagcgattcg ccgcgccggc ggctggctgc ctttcgaccg
     3001 attcatggcg ctggcgctgt atgcgccggg cctgggttac tacgcccatg gttcgcgcaa
     3061 gttcggccag ctgccctctt cgggcagcga tttcgtgacg gcgccggaaa tgacgccgct
     3121 gttcggccag gcgctggccg cgcagctggc cgatgcgctg gacgccacgg gcacagatga
     3181 agtctgggaa ttcggcgccg gctccggggc cctggcggcg cagctgctgc gggcgctcgg
     3241 aaagcgggtc cgccgctaca ccatcgtcga cctttccggc agcctgcgcg cgcgccagcg
     3301 cgaggccctc ggcggcttcg cgggcacggt ggaatgggcc ggagagctgc ccaatgccct
     3361 acgaggtgtc gtgatcggca atgaggtgct cgacgccatg ccggtcaagc tgctggcgcg
     3421 tgtcaagggg gtatggcacg agcgtggtgt cacggcggac gggccggccg gcgcgttcga
     3481 ctgggcggac cggcccaccg aactgcgccc gccggtcgat atcgcgggac cgcatgacta
     3541 tctgaccgag atccatcgcc aggccgaagc cttcatgcgc acgctggcgg accgcctggc
     3601 gcagggcgcg gccttcttca tcgactacgg attcaccgaa gccgagtact accatccgca
     3661 acgccacatg ggcaccctga tgtgccaccg cggccatctg gcggacagcg atcccctgcc
     3721 cgacctgggc ctgaaggaca tcacggccca tgtgaatttc agcgggctgg cgctcgcggc
     3781 ccaggaggcc ggtttgccca ccctcggtta caccacgcag gcgcggtttc tgatgaactg
     3841 cgggctgttg cccctcatgg agcaggcgcc gctggccgag cgggtggcgg cccaccggct
     3901 gctggccgag cacgaaatgg gcgagctgtt caaggtcatc ggcttccacc ggggagcgtt
     3961 ctgggatgcg gtggggtttc gcgacggcga ccgcacccac accctttgac cgcggcctac
     4021 actcgggagt ctgccgtcgc accgattgcc atgattcgct ggatcatcgt cattttcctg
     4081 gccctgatac tcatcagctg gttctcaccg ctgctgcagc gcatggggtt cggcaagttg
     4141 ccgggagact tgcacttcaa ggcgttcggg cgcgaatggt tcattcctct cacaaccacc
     4201 atcctcctga gcctggtggc cggggcaatt tcgaagttcg tctaggacgc ccgggatgaa
     4261 agcctgtatc gacatcggcg gtaccaaggt ggccgtgagc ctgaacagcg gtgccgggct
     4321 cgagctggtc gcgcggcgca gcgagccgac cgcgaagacc ggagcgaacg atgcgctggc
     4381 acgccaggtc atccggatgg tggacgagtc ttgcgccgaa ctcgggatcg cgccggaccg
     4441 ggtcgctcgc gccggggttt cctcctgcgg gcccttcgtc ctgcgcggcg gcctggtcga
     4501 ggtggcggcc cccaacatct gcggcggcat tgccggcccg gcgcggggct tgcccaacga
     4561 ctgggaaacg gcgttgctgg aagccccgct gcgccggcgc tttggccagg tgcgggtgga
     4621 aaacgactgc atcgccgcgc tcgaagccga gcgccgctgg ggcgcgctgc aggcattcga
     4681 tcactgtgcc tacgtcacct ggagcaccgg catcggcgtg ggactgtgcg tggacgcccg
     4741 cgtgctgcgc ggcaagaacg gaaacgcggg ccatgccggc cacatgttcg tttccgacaa
     4801 cgacgacgcg ctgtgcggct gcggcaacat cggcgatgtg gaggcgctgg tggcgggcaa
     4861 ttcgatagag cggcgattcg gcgctgacgc ggccaccttg ctgcagcgcg cccgcgaggg
     4921 cgacgcgcaa gccgaggcca ttgtggacga actgtgccgg gtgatgggcc gcgcgctgta
     4981 caacctgatc gccacgctcg atctgcagcg catcagcctc ggcggcagcg tgttctggaa
     5041 caacagcggc tacctgctgc cgcggctgca ggcgcaagtg gcgggaaaat tcccggcgct
     5101 cacggcgggc tgcgagctgg tgccggccgg tttggccgac cgagtgggag actacgcggc
     5161 gctggcgctc gtggtctgaa tccgcggccg cgcgaaacgg atgctgtcag gcatctcagt
     5221 tgctccaccg cgctgcgcgc gacccgcgac gcatgaaatc gcgcggtcaa cgcgggggcc
     5281 accgcggaac cggcttcgcc gggccgctgt tggcgccccc ttaagggggc ggcgccgaag
     5341 gcgcttcggg ggtgggcttt cacctcaggt gcagatggtc cacaggtgtt cgcacttgct
     5401 gcagcgcacc tgcgtctcct gatggctgcg gtcttcagcc accacgcgaa aacgaccgta
     5461 ctccccacag tcgccgcagc gggcctggtt ggccacctct tcggcgcgca tcagcaggaa
     5521 caggtagcgc ctgagcgccc acaagccgat gccggcagac acgatgacga aaagcacgtt
     5581 catcagcttt tcggacatgg tgccgccgtt gaaggcctcg aaactggcgg ccatcgcgac
     5641 gaagctcaag atcgcgagga tcatataggc gtggctcgat agcagctcgc gctcatacca
     5701 cttgcgaaaa cccacgcgcc gaatgccgtc cgccgggcga cggttctggt ggttgccgcg
     5761 caggaagtcc atgccgtcat catcccaaaa atccgcggcc gcggcacgca tcagggtaac
     5821 aagctgtccc tttggccgag cacctgcttc gccaccgcct gcacgcgcgc cttgtggatc
     5881 atctccccga tcgccgggcc ctcgatctct tgctgctgag cctgggccgc gatctcgtgg
     5941 gtggcgaccg cctgcgccgc ggccagcgcc gccagcagcc gctgcctcgc ggcaaatggc
     6001 ttttcctcca gccccagccg gccgcgcgcg tcgcattcgc aggccagcag cacgagatcg
     6061 aaccgctgcg gcttgcggaa ggcgtcgcaa cgctcgagca gtcgaaccag cgcggccgcg
     6121 ccgaattcgc cactgcgatg aatgttgccg tgctcccgcg ccaccacgtc ggccagctca
     6181 cggcactccg tgggcacgcg caggcgctcg cacaatttgc tgagcagccg ggcgccgcgc
     6241 tcttcgtgcc cgatgtgctt gggcaggatc tccgcgggcg tggttccctt gcccagatcg
     6301 tgcgtgagac aggcgaagcg caccgcgatc ggcgccttga gccgcgcgct catgtccagc
     6361 accatcatca ggtgcacgcc ggtgtccacc tcggggtggt actcggcgcg ctgcggaacg
     6421 ccccacagcc ggtcgacctc gggcagcagc ctggccagcg cgccggattc gcgcagcacc
     6481 tcgaacatgc gcgatggctt gtgctccatc aggccgcgcg cgagttcctg ccagacgcgt
     6541 tcggccacca gggcatcgac ctcgccggct tgcaccatgt cgcgtatcag cgccacggtc
     6601 tgcggcgcca cgacgaaatc cgcgaatcgc gccgcgaacc gtgccactcg caggatgcgc
     6661 accgggtcct cgcggaaggc ggtggtgaca tggcgcagga cgcggcgcgc caggtcggcc
     6721 cggcccccga acggatcgat cagccggccg ttctcgtcct gcgccatcgc gttgatcgtg
     6781 agatcgcgcc gggccaggtc ctgttccagc gtgacgttgg gctcggcgtg gaaggtgaat
     6841 ccgtgatagc cgcgcgcggt cttgcgctcg gtgcgcgcca gggcgtattc ctcgcgcgtg
     6901 cgcggatgca ggaacaccgg gaaatccctg cccaccggca ggtagccgtc ggcgatcatc
     6961 tcgtgcggcg tggcgccgac gaccacccag tcgtggtcgt tgaccggcag gcccaacagg
     7021 gcgtcccgca ccgcgccgcc gaccatgtac gtttgcatgg cggcagtgta cggtccctcc
     7081 aggcccgggg tgtgaacggc ccagcctggc cagcaccgtt cagcgtcctg tccggtaggg
     7141 ctcctcgaaa tcgaggaagt cctgttccgc cagggcctcc tcgatccatg ccttcacgcc
     7201 gggcaacgcg cacacgcgcc ggatgtagtc cgtgatgtgg cccggcaccg gcagcgcata
     7261 gtttttcagg cgcacgcaaa tcggtgcgaa ataagcgtcg gcgaccgaga actcgccgaa
     7321 aaggaactgg ccccccacgc tcgacctcgt ctccctgccc cccgaggggg cgctcgcacc
     7381 ttggggcggc ccggcgttgc tcatcggccc cttgtgttcc tccagcaaag cggtccacat
     7441 ggagacgatg cgttgcacat cggcgcgcac cgccggccgg tcgcgccagg ccaacgcgcc
     7501 gatctggggc aacgatgcct cgacgttcat cgggcaatgg ccgcgcaggc cggtaaaacc
     7561 cgaatgcatt tcggcgcaga tgctgcgggc gcgggcccgt gctttcgcat cacgaggcca
     7621 cagctgccgg tccggaaact tctcggcgac gtattcggcg atggccagcg tgtcccagac
     7681 cacgaacccg tcgtcgacca gcaccggcac ccggccggtc gggctgagat ccttgagggt
     7741 gcgcttgaag gccgaatcgg cgtcgagcga atcgaagcgg accatcacct cctcgaactg
     7801 gatgcctgcc tgcttgagca gcacccagct tcgcatggac caggacgaat agttcttgtt
     7861 gccgatgtag agtttgagca tggaggtctc ctggaggtcg ccgagccccg catgctaccg
     7921 cccccgccgg ccggattgat gcgcaaaaat gcggccattg atgtcgcgtc gggcaggttc
     7981 agtgctcgcg atccgcctcc gccccctgcg cgccgcgccc gcgcatcaag cgccgaatgg
     8041 gcccttcgag ggcgaggatg gccagcgcga gcatcgtgct gagcactgcg gtggtctcac
     8101 ggcccacgcc ggcggccatg ccgatcgccg ccgtcaacca gatgcctgcg gccgtggtca
     8161 agccgcgtac ctggtcgtca cccgacttga gtatggtgcc ggcgcagaga aacccgatcc
     8221 cggcgatgac gccctgcata acgcggctca ggtcggcggc ctccatgccg gcttgctgcg
     8281 gaaccaggac gaacatggcc gctcccaggg ccaccagcat gtgggtgcgc acgcccgcct
     8341 ccttgccgtg gcgttcgcgc tgcagcccca gcaagccgcc cagcagcgcg gccagcagca
     8401 gccgcaacac cagccgcgtg aactgtggca cgtccggcaa gtcggagaac tccgatgcaa
     8461 tcgtttcgat gaccgcgtgc caccatgcca cggcgagtcc tcagggcagc tcgccgttag
     8521 gctcgggcag gctggcgtcg cgcgggccga ccatgcccag gcgcgccttg agcgactgcg
     8581 gctggccgct gaggatggcg gcgtagatcg tggtgttggc cagcactttc ttcacgtagt
     8641 cgcgcgtttc gccgaatggc acgttctcgg cccaggccgc cgcttcgatc acggcgccgt
     8701 tgcgccagct gcgcggccgg cccggtccgg cgttgtaggc ggcagcggcc agcggcatgg
     8761 agccgtcgaa gtcatcgagc accagcttca ggtagccggt gccgatcgcg atgttggttt
     8821 cgcggtccga gagctggtgc ggcgcgaagt tggccagccc gatcttgcgc gccgtccagc
     8881 gggccgtggc cggcatcacc tgcatcaggc ccgaggcgcc cacgttggaa cgcgcatcca
     8941 tgatgaagcg gctttcctgg cggatcaatc cgtacacgta ggccgggtcg agcccgatct
     9001 cctggctgcg cttgaccacc gattcgcgga acggcatggg gaagcgctgc tcga
//
LOCUS       DASWIY010000078         6165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1158209, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000078 DASWIY010000000
VERSION     DASWIY010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158209"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..936)
                     /gene="rsmA"
                     /locus_tag="VGA59_02990"
     CDS             complement(166..936)
                     /gene="rsmA"
                     /locus_tag="VGA59_02990"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX9718670.1"
                     /translation="MKHIPRKRFGQHFLTHSGVIDAIVRAIDPRPGQAMVEIGPGLAA
                     LTQPLAERLGKLAVIELDRDLAARLRGHSQLAVTEADVLKVDFSQLARSLGVPKIRVV
                     GNLPYNISTPILFHLLDFVNSIEDQHFMLQKEVIDRMVARPATSDYGRLSVMLQWRYA
                     MENVLLVPPEAFEPPPRVDSAVVRMVPLAQPAAVDPALLSQLVQVAFSQRRKLLRHTL
                     GKWLEQRNFSGAFDTQRRAEEVPVEEYLALVAAVSTQA"
     gene            complement(1074..2447)
                     /locus_tag="VGA59_02995"
     CDS             complement(1074..2447)
                     /locus_tag="VGA59_02995"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9718671.1"
                     /translation="MYQRLYAPWLACLALAVALPAASQGLRATPQPGAARPAPRAADT
                     SPRPADYIVAVVNSEPITNNEVRSRMIRFEQQLAQQGQPVPPRAQLARQVVERLISEK
                     AQLQLARETGIKVEESTVDQAEQNVARQNLIEVPELRRRLAADGIALSQFREDLRNQL
                     LLTRLRERELEPRVKVTDLDVERYLREQQAGGGDAAPVELNLAQILVAVPENAPEAQI
                     AGLRAKAERVQQRARAGEDFAKLAREFSDSPGAAASGGQVGLRSADRYPPLFVQAIRA
                     LPDGGVADLVRSGAGFHVIKVIEKRSGGVPGIHVVQSHARHILLRPGRQLSEAAARQR
                     LADFKKRIEAGQADFAQLAREFSQDASAANGGDLGWANPGLFVPEFEDAMNALAPGQI
                     AEPLLSRFGVHLIQLLERRQATLTPRERREMARQLVREKKLDEAYAQWAQDVRGRAYV
                     ELREPPQ"
     gene            complement(2465..4768)
                     /locus_tag="VGA59_03000"
     CDS             complement(2465..4768)
                     /locus_tag="VGA59_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041836136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEX9718672.1"
                     /translation="MAWAVLNTGAARAQEQPPLSLKPSPALREDIPDPVRERLPTYLS
                     GERVFGRTDLETVIEGQAQLRRGDTVIRADRLEYQHPDDLARARGNVRINRAGDVFEG
                     PLLELKVDAFEGFFNQPRYRFLRNDAYGEADRVDFIDDKRAIIRNATYTTCQRKPGPS
                     WMPDWILRAASIRIDTEEEVGQASDAVLSFMGLPILPVPELSFPLSERRKSGLLPPTI
                     GLDSLNGAELTIPYYWNIAPNRDATFYPALMSKRGIDLGGEFRYLERDYAGQLRANYM
                     PNDRLRQKGRWGYAFRHDAAVGAGLPTGPLGLNLNLNRVSDDNYWRDFSGTTASLTQR
                     LLANDGNLTWARGDFSVAARALKWQTLQDVTSPIVPPYDRLPQVVARHSRANLPGGLA
                     TYVEADYTRFEGDRMLTGQPNAHRSFTLAQISRPWQAPGWFVIPKLQLHASQYQFDAA
                     LTNGARSATRTVPTFSLDSGLVFERDTSYFGRAFRQTLEPRAFYVHTPFRDQSLLPNY
                     DSGANDFNFATIYTENAFGGHDRIADNNLLTLGASTRLLDPDTGAEVSRFGIAQRLRF
                     KDQRVTLPGQAPVSERLSDLLFGASVNWSPKWSIDSTLQYNPKTRRSIRSTIGGRYSP
                     GNYRVVSAAYRLQRGSSEQLDIGWQWPINDLWGDKGQNLGPGRGEGEGRWYSVGRLNF
                     SLQDKRLVDALVGLEYDAGCWLGRIVFERLQSSTSTSNKRILFQLEFVGFSRLGSSAL
                     QALKENIPRYQYLREQVTIPSRFSNYD"
     gene            4891..6060
                     /locus_tag="VGA59_03005"
     CDS             4891..6060
                     /locus_tag="VGA59_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX9718673.1"
                     /translation="MSELANPSLPPATPVAWPDPQREAAFMRWLDGIAGPHGLLPASL
                     RPASADASFRRYLRIDVAPTLDTDVSSLPPEGAVSPRGGPAAKRHGGSRIIMDAPPDK
                     EDCRPFAKVSRLMGEAGLNVPRVLEWQEASGFLLLDDLGTQTMIEAIAPADAASNRPL
                     YLRALDALVAWQAASRPGVLPAYDHALLARELALFPDWYLERHRGVKVEGRLRETLDA
                     MFKLIIERNLAAPSVFVHRDFMPRNLMIPGDEAEPRLGVLDFQDAVQGPVTYDIASLM
                     RDAFLSWDEEFVLDITVRYWQRALKAGLPVDPDFGEFYRAVEWMGLQRHLKGAGIFAR
                     LTLRDGKPRYLADTPRFIAYIRATAGRYRELAPLLRLIDEVEGTQAVSGYAFGRI"
     gene            6071..>6165
                     /locus_tag="VGA59_03010"
     CDS             6071..>6165
                     /locus_tag="VGA59_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX9718674.1"
                     /translation="MPRFHCAAPLVTGQSLHLPAGAARHVQVLRL"
BASE COUNT          934 a         2078 c         2107 g         1046 t
ORIGIN      
        1 gcatgagcag ccccttcaac gcggggtatt ggttggcagc agcttagtaa gggctgtcat
       61 ccctggcttg acccggtatc catgaattcg gagttctgag tcatggattg cgggtcaagc
      121 ccgcaatgac aaagccatga agcccgtgca tagccacggg ctttttcacg cctgcgtcga
      181 aacagcagct accaaggcaa gatattcctc gaccggcacc tcttccgcgc ggcgctgcgt
      241 atcgaaggcg ccgctgaagt tcctttgctc cagccacttg cccagcgtgt gccgcagcag
      301 cttgcggcgc tggctgaagg cgacctgcac cagttgcgac aacagcgccg ggtccaccgc
      361 ggcgggctgc gccagcggca ccatgcgcac cacggcactg tcgacgcggg gcggcggctc
      421 gaatgcctcg ggcggaacca acagcacgtt ttccatcgcg tagcgccact gcagcatcac
      481 cgacaggcgt ccgtagtcgc tggtcgcggg cctggcgacc atgcggtcta tcacttcctt
      541 ttgcagcatg aagtgctggt cctcgatgct gttcacgaaa tccagcaggt ggaacaggat
      601 cggcgtggag atgttgtagg gcaggtttcc caccaccctg attttcggga cgcccagcga
      661 gcgcgccagc tgggagaagt ccaccttgag cacgtcggcc tcagtcacgg ccagctggct
      721 gtggccgcgc agccgcgccg cgagatcgcg atccagttcg atgaccgcca gcttgccgag
      781 ccgctccgcc aggggctgcg tcagcgccgc cagtccgggg ccgatttcca ccatcgcctg
      841 gccgggccgc ggatcgatcg cgcgcacgat cgcgtcgatc acgccggagt gggtcaggaa
      901 atgctggccg aagcgcttgc gggggatgtg tttcatctgc gctcgtcatt gcggactcga
      961 tccgcaatcc atgtggtccg tatcaaaggc cggcgagggg catggatccc ggatcgggcc
     1021 tgccccggac ttgatccggg gtccgggatg acacgctagt tatgcggcaa aggctattgc
     1081 ggcggttccc gcaactccac gtaggcgcgg ccgcgcacat cctgcgccca ttgggcgtaa
     1141 gcctcgtcga gctttttctc gcgcaccagc tggcgcgcca tctcgcgccg ctcgcgcggc
     1201 gtcagcgtcg cctggcggcg ctcgagcagc tggatcaggt gcacgccgaa ccgcgagagc
     1261 aggggctccg cgatctggcc gggcgccagg gcgttcatcg cgtcctcgaa ttccggcacg
     1321 aacagcccgg ggttggccca gcccaggtcg ccgccgttgg ccgcgctcgc gtcctgcgag
     1381 aactcgcgcg ccaactgcgc gaagtcggcc tggccggcct cgatgcgctt cttgaaatcg
     1441 gccaatcgct gccgcgccgc ggcttcgctg agctggcgac ccgggcgcaa caggatgtga
     1501 cgggcgtggc tttgcacgac atggataccg ggcacgccgc cgctgcgctt ttcgatcacc
     1561 ttgatgacgt gaaagccggc gcccgatcgc accaggtcgg cgacaccgcc gtcgggcagc
     1621 gcccggattg cctgcacgaa cagcggcggg tagcggtcgg cgctgcgcag gccgacctgg
     1681 ccgccgctgg cggcggcgcc cggcgagtcc gaaaactccc gcgccagctt ggcgaagtcc
     1741 tcgccagccc gcgcgcgttg ctgcacacgc tcggccttgg cgcgcaaacc cgcgatttga
     1801 gcctcgggcg cgttctcggg cacggccacg agaatctggg ccaggttcag ctcgaccggg
     1861 gcggcgtcgc cgccgccggc ctgctgctcg cgcaggtacc gctccacatc gaggtcggtc
     1921 acctttaccc gcggctcgag ctcgcgctcg cgcaggcgcg tgagcagcag ctggttgcgc
     1981 aggtcctcgc ggaactgcga cagcgcgatg ccatccgcgg ccagccggcg gcgcagctcc
     2041 ggcacctcga tcaggttttg ccgcgccacg ttctgctcgg cctggtccac cgtggattcc
     2101 tccactttga tgccggtctc gcgcgccagc tgaagctgtg ccttttcgct gatcagccgc
     2161 tccaccacct ggcgggccag ctgcgcgcgt ggcggcacgg gctggccctg ctgggcgagc
     2221 tgctgctcga aacgaatcat cctcgagcgc acctcgttgt tggtgatggg ctccgagttg
     2281 acgaccgcca cgatgtagtc ggccggccgc ggactcgtgt cggcggcacg aggagccggc
     2341 cgcgccgcgc ccggctgcgg ggttgcacgc aggccctgcg aggcggccgg cagggccaca
     2401 gccagggcga gacaggccag ccacggggca tagaggcgtt gatacatggt gctcactcgt
     2461 cttgctagtc gtaattgctg aagcggctgg ggatcgtcac ctgctcgcga aggtattgat
     2521 aacgggggat attctccttg agcgcctgca gggcgctcga gcccagccgc gaaaagccca
     2581 cgaattcgag ctggaacagg atgcgcttgt tcgatgtcga cgtgctgctt tgcagccgtt
     2641 cgaacacgat acggcccagc cagcagcccg cgtcgtactc cagccccacc aatgcgtcga
     2701 ccagccgctt gtcctgcagg ctgaaattca gccgccccac actgtaccag cggccttcac
     2761 cctcgccccg gccggggccc agattctggc ccttgtcgcc ccagaggtcg ttgatgggcc
     2821 attgccagcc gatgtcgagc tgctcgctcg atccgcgctg cagccggtaa gccgcgctga
     2881 cgacgcggta attgcccggg ctgtagcgcc caccgatcgt ggagcggatg gaacgccggg
     2941 tcttggggtt gtattgcaag gtggaatcga tgctccactt cggggaccag ttgaccgaag
     3001 cgccgaacag cagatccgac aaacgctcgc tgacaggcgc ctgccccggc agtgtcacgc
     3061 gctggtcctt gaagcgcagc cgctgggcga tgccgaaacg gcttacctcg gcgcccgtgt
     3121 cggggtcgag cagccgcgta ctggcgccca aggtgagcag gttgttgtcc gcgatccggt
     3181 catggccgcc gaaagcgttc tcggtgtaga tggtggcgaa gttgaagtcg ttggcgcccg
     3241 agtcgtaatt gggcagcagg ctctggtcgc gaaacggcgt gtgcacgtag aaggcgcgcg
     3301 gctccagggt ctggcggaag gcgcgcccga agtagctcgt gtcgcgctcg aataccaggc
     3361 cgctgtccag gctgaaagtc ggcacggtgc gggtggccga ccgcgctccg ttggtcaggg
     3421 ccgcgtcgaa ctggtactgg ctggcatgca gctgcaactt gggaatgacg aaccagcccg
     3481 gcgcctgcca cggccggctg atttgcgcca gcgtgaagct gcggtgcgcg ttgggctggc
     3541 cggtcagcat gcggtccccc tcgaagcgcg tgtagtcggc ctcgacatag gttgccaggc
     3601 cgccgggcag gttggcgcgg gaatggcgcg ccaccacctg gggcaggcgg tcatagggcg
     3661 gcacgatggg cgacgtcaca tcctgaaggg tctgccactt caaggcccgg gcggcgacag
     3721 aaaagtcgcc ccgcgcccag gtcaggttgc cgtcgttggc cagcaggcgc tgcgtgagcg
     3781 acgccgtggt gcccgagaag tcgcgccagt agttgtcgtc gctcacccgg ttgaggttga
     3841 ggttcagtcc gagcggtccg gtcggcaggc cggcgcccac tgcggcatcg tgcctgaacg
     3901 cgtagcccca gcggcccttc tggcgcagcc ggtcattggg catgtagttg gcccgcagct
     3961 gcccggcata gtcgcgctcc aggtagcgga actcgccgcc caggtcgatc ccgcgcttgc
     4021 tcatcaatgc gggatagaac gtggcatcgc ggttgggcgc gatgttccag taatagggaa
     4081 tcgtcagttc ggcgccgttc aggctgtcca gcccgatggt gggcggcagc aggcccgact
     4141 tgcgcctttc cgacagcgga aagctgagct cgggcaccgg caggatcggc agccccatga
     4201 agctcagcac cgcgtcgctg gcctgtccga cttcttcctc ggtgtcgatg cggatgctcg
     4261 ctgcgcgcag gatccagtcg ggcatccagc tcgggcccgg cttgcgctgg caggtggtat
     4321 aggtggcgtt gcggatgatc gcccgcttgt cgtcgatgaa atcgacccgg tcggcctcgc
     4381 cgtaggcgtc gttcctcagg aaccggtagc gcggctggtt gaagaaacct tcgaacgcat
     4441 cgaccttgag ttcgagcaac gggccttcga acacatcgcc cgcccggttg atgcgcacgt
     4501 tgccgcgcgc cctggcgagg tcatcggggt gctggtactc cagccggtcg gcgcggatca
     4561 cggtatcgcc gcggcgcaac tgcgcctgcc cttcgatcac ggtttccagg tcggtccggc
     4621 cgaagacgcg ctcgcccgac aggtaggtgg gcagccgctc gcgcaccgga tcgggaatgt
     4681 cctcacgcag cgcggggctg ggcttgagcg acagcggcgg ctgctcctgg gcgcgggccg
     4741 cgccggtgtt cagcacggcc caggccatga gcgccaccgg tgtcagggca aagcgggcgc
     4801 gaaacgcctt cgaattcaag tgcatcaagg tagttcttgc ggcggcatgg cgcccggccg
     4861 gcaggactgg atttgtagaa tggattatcc atgagcgaac tcgcgaaccc gtccctgccg
     4921 cccgccacgc ccgtcgcgtg gcccgatccc caacgcgaag ccgctttcat gcgctggctg
     4981 gacggcatcg cgggcccgca tggcctgctg cccgcgagcc tgcggccggc ctcggccgat
     5041 gcgagcttcc ggcgctacct gcgcatcgac gtggccccca cgctcgacac ggacgtgtca
     5101 tcgctgcccc ccgagggggc tgtttcgcct aggggcggcc cggcggcgaa acgtcatggc
     5161 ggcagccgca tcatcatgga cgcgccgccc gacaaggagg actgccgtcc gttcgcgaag
     5221 gtctcgcggc tgatgggcga ggccggcctg aacgtgccgc gcgtcctcga gtggcaggaa
     5281 gcgtccggct tcctgctgct ggacgacctg ggcacgcaga ccatgatcga agcgatcgcg
     5341 ccggccgacg ccgcgtccaa ccggccgctc tacctgcgcg ccctcgacgc cctggtggcg
     5401 tggcaggccg cgtcgcgacc gggcgttctg cccgcctacg accatgcttt gctggcccgt
     5461 gaactggcgc tctttcccga ctggtacctg gagcggcacc gcggggtgaa ggtcgagggc
     5521 cggctgcgcg aaacgctcga tgccatgttc aagctgatca tcgagcgcaa cctggccgcg
     5581 ccgtccgtct tcgtgcaccg cgatttcatg ccgcgcaacc tgatgattcc cggcgacgag
     5641 gccgaaccgc gcctgggcgt gctggatttc caggatgcgg tgcagggacc ggtcacctac
     5701 gacatcgcca gcctgatgcg cgatgccttc ctcagctggg acgaggaatt cgtgctcgat
     5761 atcaccgtgc gctactggca gcgcgcgctc aaggccggcc tgccggtcga cccggatttc
     5821 ggcgagttct accgcgcggt ggaatggatg ggcctgcagc gccacctcaa gggggccggt
     5881 atcttcgcgc ggctgaccct gcgcgacggc aagcccaggt acctggccga cacgccgcgg
     5941 ttcatcgcct acatccgcgc gacagccggc cgctaccgcg agctcgcgcc cttgctgcgg
     6001 ctgatcgacg aggtcgaagg cacgcaggcc gtctccggct atgcgttcgg ccggatctga
     6061 ccggcagcgg atgccgcgct ttcactgcgc cgcgccgctg gtcaccggcc aatcgctgca
     6121 cttgcccgcg ggggcggccc ggcatgtgca ggttctgcgc ctgca
//
LOCUS       DASWIY010000079         4894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_49228, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000079 DASWIY010000000
VERSION     DASWIY010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49228"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VGA59_03015"
     CDS             <1..140
                     /locus_tag="VGA59_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HEX9718675.1"
                     /translation="RLALTFEIIYGHAFKPSPRVGLSDRSAVSLEDMRAMLQSGKTPR
                     G"
     gene            235..720
                     /locus_tag="VGA59_03020"
     CDS             235..720
                     /locus_tag="VGA59_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEX9718676.1"
                     /translation="MPNPVFRFATVHGQNIRWFLKRNCSVTPVQLGWLYGSLCVVSLG
                     IGTFFWYQGAVLVLPFAALELAAVGAAFLAYARHATDGETISLEGRQLVVELESAGHL
                     QRAEFDRDWVRVEPGVSDRSLVELSARGRRVNVGRYLRPELRPVLAQEIRRALRDAQR
                     R"
     gene            800..1960
                     /gene="coxB"
                     /locus_tag="VGA59_03025"
     CDS             800..1960
                     /gene="coxB"
                     /locus_tag="VGA59_03025"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX9718677.1"
                     /translation="MTSISNKLAALLLALGAFSPAAHAVNDLPGGPAVRQLNLHAAAS
                     RIAVEQAWLHWFMLITSTVIFIAVFSVMFYSIWKHRKSVGHKAANFHESVTVEMIWTV
                     IPFIIVIGMALPATKVVVAAKDTSNADLTIKATGYQWKWGYDYLNGEGEGLSFLSALD
                     RTHREMSNEGAKGEMPDDYLLKVDNPLVVPVNKKIRIITTANDVIHAWGVPALGVKQD
                     AIPGFVRDTWFRSEKTGDFYGQCYELCGKEHAYMPIHVKVLSAQEYSGWVDTEKKKLA
                     ARQDDPNKVWALDDLVKRGEKVYAANCAACHQATGMGAGPIKPLDRSPLVLDPDKTKQ
                     IHIVLNGQAAMPSWKQLSDTDLAAVISYTKNSWSNKTGQIVQPAEVLAQRGK"
     gene            1995..3653
                     /gene="ctaD"
                     /locus_tag="VGA59_03030"
     CDS             1995..3653
                     /gene="ctaD"
                     /locus_tag="VGA59_03030"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX9718678.1"
                     /translation="MSAVLEHPDHAPGDHHDHAPAGWRRWVYATNHKDIGTMYLLFAF
                     TMLMVGGVLALMIRAELFQPGLQLVNPELFNQLTTMHGLIMVFGAIMPGFVGFANWMI
                     PMQIGAADMAFARMNNFSFWLLIPAAVMIVTSFFMPGGAPAAGWTLYAPLTLQMGPSM
                     DMGIFALHIMGASSIMGSINIIVTILNMRAPGMTLMKMPMFAWTWLITAYLLIAVMPV
                     LAGAITMTLTDRHFGTTFFNPAGGGDPVMYQHIFWFFGHPEVYIMILPAFGIISQVVP
                     AFSRKRLFGYASMVYATSSIAILSFIVWAHHMFTTGMPVTGQLFFMYATMLIAVPTAV
                     KIFNWIATMWQGSMTLETPMLFAIGFIFVFTIGGFTGLILAMAPIDILVQDTYYVVAH
                     FHYVLVAGSLFAMFAGFYYWAPKWTGVMYNEAMGKWHFWLSIVTFNITFFPMHFLGLA
                     GMPRRYADYPMQFADFNALASIGGFGFGLAQVFFFFAVVLPVMRGKGAKAPQRPWNDP
                     DGKGAEGLEWEVPSPAPFHTFETPPRLNITATKVLDARVQPEHH"
     gene            3668..3784
                     /locus_tag="VGA59_03035"
     CDS             3668..3784
                     /locus_tag="VGA59_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HEX9718679.1"
                     /translation="MATTREQRKSNIRMALILASVAAVFFAGIVVKMAWLAR"
     gene            3795..4400
                     /locus_tag="VGA59_03040"
     CDS             3795..4400
                     /locus_tag="VGA59_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX9718680.1"
                     /translation="MSIRGENSRMVGKLAVVAAGMFAFGYALIPIYQRICEVTGINIL
                     SLSERRVPGGASTGGDVKLGNSQVDTSRTITVEFDANVRGPWEFKPGQRSVQVHPGEL
                     ATVMYEFQNVQNRTMAAQAIPSYAPRQAAAHFNKLECFCFQQYTLAPGEKRQWPVAFV
                     IDPKLSKDVKTITLSYTFFEVGGKMPPPPGATVAAGMEPGK"
     gene            4397..4612
                     /locus_tag="VGA59_03045"
     CDS             4397..4612
                     /locus_tag="VGA59_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HEX9718681.1"
                     /translation="MSPNTPPATARRGSFLRTVKAVAWSFLGIRKNSEYQEDLGKLNP
                     FHIIAVALVGVALLVAGLIALVSWVAK"
     gene            4641..>4894
                     /locus_tag="VGA59_03050"
     CDS             4641..>4894
                     /locus_tag="VGA59_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX9718682.1"
                     /translation="MSTSAPGTKPYYFVPQPSRHPAFAAFGLFWIILGAQQWINGAGW
                     GRYLLFFGFAWLFGVLFQWFRDTIRESEGGRYGHKIDLSFR"
BASE COUNT          871 a         1579 c         1537 g          907 t
ORIGIN      
        1 gccggctggc gctgacgttc gagatcatct acggccacgc cttcaagccg agccccaggg
       61 tggggctcag cgaccgcagt gccgtctcgc tcgaggacat gcgggccatg ctgcaatcgg
      121 gcaagacgcc ccgcggctga cctgcttgct tattgtccaa aatcaatgca gcgcgggccc
      181 agatgtggca agcgcggccg cgctacaatc cccggttgat tttcgagcgt caccgtgccg
      241 aatccggtat ttcgtttcgc caccgtccat ggccagaaca tccgctggtt cctgaagcgc
      301 aactgctcgg tcacgccggt ccaattgggc tggctctatg gctcgctgtg cgtcgtttcg
      361 ctcggaattg gtacgttttt ctggtaccag ggggctgtgc tggtccttcc gtttgcggcg
      421 ctcgagctgg ccgcggtcgg cgccgcgttc ctggcgtatg cgcggcatgc gaccgatggc
      481 gagacgattt cgctggaggg acggcaactg gtggtcgagc tggagagcgc gggccacctt
      541 cagcgggccg agttcgatcg cgactgggtt cgggtcgaac ccggcgtgag cgaccgctca
      601 ctggtcgagt tgtcggcacg gggccggcgg gtgaacgtgg ggcgttacct gcgacccgag
      661 ctcaggccgg tgctggcgca ggagatcaga agggctttgc gcgacgcgca gcgccgttga
      721 ttttggcagg gcagttcgtg gactccgtcc cgaagggggc ggcgggaatt tgattgagga
      781 ttcaaggcta gtgaacacca tgacgagcat ttcgaacaag ttggccgcgc tgctgctcgc
      841 cctcggcgcg ttcagcccgg cggcccatgc ggtcaatgac ctgcccggcg gccctgccgt
      901 gcgccagctc aaccttcatg ccgcggcctc gcgcatcgcc gtggagcagg cctggctgca
      961 ctggttcatg ctcatcacca gcacggtgat cttcatcgct gttttcagcg tgatgttcta
     1021 ttcgatctgg aagcaccgca aatcggtcgg ccacaaggcc gccaatttcc acgagtcggt
     1081 caccgtcgag atgatctgga ccgtcatccc cttcatcatc gtcatcggga tggccttgcc
     1141 cgccaccaag gtggtggtcg ctgccaagga cacgagcaac gccgacctga caatcaaggc
     1201 caccggctac cagtggaagt ggggctacga ctacctcaac ggcgaagggg agggcctgag
     1261 cttcctgtcc gccctggaca gaacgcaccg cgaaatgtcg aacgaaggag ccaagggcga
     1321 aatgcccgac gactacctgc tcaaggtcga caacccgctg gtggtgcccg tcaacaagaa
     1381 aatccgcatc atcaccaccg ccaacgacgt catccacgcc tggggcgtgc ccgcgctggg
     1441 cgtcaagcag gatgcgatac ccggcttcgt gcgcgacacc tggtttcgca gcgaaaagac
     1501 cggtgacttc tacggacagt gctacgagct gtgcggcaag gagcatgcct acatgccgat
     1561 ccacgtgaag gtgctgtcgg cgcaggaata cagcggctgg gtcgacaccg aaaagaagaa
     1621 actcgccgcc aggcaggacg acccgaacaa ggtctgggcg ctggacgacc tggtcaagcg
     1681 cggcgaaaag gtgtacgccg cgaattgcgc ggcctgccac caggcaaccg gcatgggcgc
     1741 cgggccgatc aagccgctgg accgctcgcc gctggtgctg gaccccgaca agaccaagca
     1801 gatccacatc gtcctgaacg gccaggcagc catgccgtca tggaagcagc tgtcggatac
     1861 cgacctggcg gcggtgatca gctacaccaa gaacagctgg tcgaacaaga ccgggcagat
     1921 cgtgcagccc gccgaagtcc tggcccagcg cggcaagtaa acgcgcggcg agaagaaaga
     1981 agcaaggaaa ccagatgagc gccgtactcg agcatcccga ccacgccccg ggcgaccacc
     2041 acgatcacgc gcctgcagga tggcgccgct gggtctacgc caccaaccac aaggacatcg
     2101 gcacgatgta cctgctgttc gccttcacca tgctgatggt gggcggtgtg ctggccctca
     2161 tgattcgcgc cgaactgttc cagcccgggc tgcagctggt caatcccgag ctgttcaacc
     2221 agctcaccac catgcacggc ctgatcatgg tgttcggcgc gatcatgccg ggcttcgtcg
     2281 gattcgcgaa ctggatgatc ccgatgcaga tcggcgcggc cgacatggcg ttcgcgcgga
     2341 tgaacaactt cagcttctgg ctgctgatcc cggccgcggt catgatcgtc acctccttct
     2401 tcatgcccgg cggggcgccc gccgcgggct ggacgctgta tgcgccgctg acccttcaga
     2461 tgggcccgtc catggacatg ggtatcttcg ccctgcacat catgggcgcg tcctccatca
     2521 tgggatcgat caacatcatc gtcaccatcc tgaacatgcg cgctccgggc atgacgctga
     2581 tgaagatgcc gatgttcgcc tggacctggc tgatcacggc ctacctgctg atcgcggtga
     2641 tgccggtgct ggccggcgcc atcaccatga cgctcaccga ccgccatttc ggcaccacct
     2701 tcttcaatcc cgccggcggc ggcgatccgg tgatgtacca gcacatcttc tggttcttcg
     2761 gccaccccga ggtctacatc atgatcctgc cggccttcgg catcatcagc caggtggtcc
     2821 cggcgttctc gcgcaagcgc ctgttcggct acgcctccat ggtctacgcg acctcgtcga
     2881 tcgcgatcct gtcgttcatc gtgtgggccc atcacatgtt caccaccggc atgccggtca
     2941 ccggccagct gttcttcatg tacgccacga tgctgatcgc ggtgcccacg gccgtgaaga
     3001 tattcaactg gatcgccacc atgtggcaag gttccatgac gctggagacg ccgatgctgt
     3061 tcgccatcgg attcatcttc gttttcacca tcgggggttt caccggcctg atcctggcga
     3121 tggcgcctat cgacatcctg gtgcaggaca cttattacgt ggttgcccat ttccattacg
     3181 tgctggtggc cggctcgctg ttcgccatgt tcgcgggctt ctactactgg gcgccgaaat
     3241 ggaccggggt catgtacaac gaggccatgg gcaagtggca cttctggctc agcatcgtga
     3301 ccttcaacat cacgttcttc ccgatgcact tcctgggcct ggccggcatg ccccgccgct
     3361 acgccgacta cccgatgcag ttcgccgact tcaacgcgct tgcgtccatc ggcgggttcg
     3421 gattcgggct ggcgcaggta ttcttcttct tcgccgtcgt cctgcccgtg atgcgcggca
     3481 agggtgcgaa agcgccgcag cgtccgtgga acgatcccga cggcaagggc gcggaaggcc
     3541 tggaatggga agtgccctcg ccggccccct tccacacttt cgagacgccg ccgcgcctga
     3601 acatcaccgc gacgaaggtg ctggacgcgc gcgtgcagcc cgaacatcat tgaggcgggg
     3661 ccggaccatg gcgacgaccc gcgagcaacg caaaagcaac atccggatgg cgctgatcct
     3721 ggcgtcggtg gcggcggtgt tcttcgccgg catcgtcgtc aagatggctt ggctggcccg
     3781 gtgaccccgg catcatgagt atccgcggcg aaaattcgag gatggtgggc aagctggccg
     3841 tggtggcggc cggcatgttc gccttcggct acgcgctgat cccgatctac cagcgcatct
     3901 gcgaggtgac cggcatcaac atcctgtcgc tgtcggagcg gcgggttccc ggcggcgcaa
     3961 gcaccggcgg cgacgtgaag ctgggcaaca gccaggtgga taccagtcgc accatcacgg
     4021 tggagttcga cgccaacgtg cgcggtccgt gggagttcaa gccgggccag cgttccgtgc
     4081 aggtgcaccc gggcgaactg gccaccgtga tgtacgaatt ccagaacgtg cagaaccgca
     4141 ccatggccgc gcaggcgata ccgagctacg cgccgcgcca ggccgccgcg cacttcaaca
     4201 agctcgagtg tttctgcttc cagcagtaca cgctggcgcc tggcgaaaag cggcagtggc
     4261 cggtcgcctt cgtgatcgat ccgaagctgt cgaaggacgt gaagaccatc acgctgtcat
     4321 acacgttttt cgaagtcggc ggcaaaatgc cgccgccgcc cggcgccact gtcgcggccg
     4381 ggatggagcc gggcaaatga gcccgaatac gccgcccgcg acagcgcgcc ggggctcttt
     4441 cctgcgaacg gtcaaggccg tggcctggtc tttcctgggc atccgcaaga acagcgagta
     4501 ccaggaagac ctgggcaagc tcaacccgtt ccatatcatc gccgtggccc tggtgggcgt
     4561 ggccttgttg gtggccggcc tgatcgccct ggtcagctgg gtggcgaaat gaatgcaaga
     4621 aatctggatc aggagatgag atgagtacaa gcgcacccgg taccaagcct tattacttcg
     4681 tgccacagcc ttcgcgccat ccggcgttcg ccgctttcgg gctgttctgg atcattctcg
     4741 gcgcgcagca gtggatcaac ggcgccggct ggggccggta cctgctcttc ttcggcttcg
     4801 cctggctgtt cggcgtcctg ttccagtggt ttcgcgacac catccgcgag agcgaaggcg
     4861 ggcgctacgg ccacaagatc gacctctcgt tccg
//
LOCUS       DASWIY010000080         9343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1158980, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000080 DASWIY010000000
VERSION     DASWIY010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158980"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1463)
                     /locus_tag="VGA59_03055"
     CDS             complement(105..1463)
                     /locus_tag="VGA59_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX9718683.1"
                     /translation="MATELHPALAAIRKSGADKVKVACSDIDGVLRGKYLHKDKFEGA
                     ADGGFGFCDVVFGWDCGDVTYDNAQVTGWQHGFPDALARLDLDTHRRVPWDGGVDFFL
                     GEFINTDGSPSPICPRQTLKRVLRRAEKLGFQVMTGMEYEWFNFRETPQSWAAKNGVR
                     PETLTPGMFGYSLLRVSQNREFFNALMDEMLAFGVPIEGLHTETGPGACEAAISFSEA
                     LEQADRAVLFKTGAKEIGVRFGIMPSFMAKWSQQYPGCSGHIHQSLTDGKTNLFFDTK
                     SSRKMSPLFESYLAGQVACLMEFAPMFWPTINSYKRLVDGFWAPVKPTWGMDNRTASF
                     RVIAGSPKGTRLETRCPGADINPYLAMAAVIAAGLHGVEKGLKLTTAPITGTNEGAEN
                     IPRAPRTLIETTRNFSNSTIARDFLGDTFVEHFAATREWEWRQWLDGVTDWELKRYFE
                     II"
     gene            complement(1397..1723)
                     /locus_tag="VGA59_03060"
     CDS             complement(1397..1723)
                     /locus_tag="VGA59_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718684.1"
                     /translation="MLRDWGSWDSDPRYEFQAFYSPARQCGFILYPGKLTRLETFRVG
                     CIGAIGPNEMEQAVQAVALALENMGIAPGEPAIWITAERKTTRWQPNSTPRSPRSANP
                     APTRSR"
     gene            1754..1951
                     /locus_tag="VGA59_03065"
     CDS             1754..1951
                     /locus_tag="VGA59_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718685.1"
                     /translation="MNAALHPTIALLQSSSLTPVVQQEIERAIPQGEHAPGSKLIEAN
                     LAGQMGVSRGIKLHVEAKRYR"
     gene            1948..2793
                     /locus_tag="VGA59_03070"
     CDS             1948..2793
                     /locus_tag="VGA59_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9718686.1"
                     /translation="MTLTWPVVAAVLFGALLHAGWNALVKSSSDKALDTAVIHLLGSL
                     VALPIALVVGWPPAASWPFILTSVVIHIGYYIALTGAYKHGDLGLTYPLMRGTAPLLV
                     ALSAAATLGETLSPLSWAGVLGVSCGVLALGLSRHALDSPRAVGFALANAVVIAVYTV
                     VDALGVRASGHALQYVVALFALDGWPFALMVFARRGGATVWPYARVRWPVASLAAAAS
                     LGSYGIALWAMTRAPVATVAALRETSVLFAALLGTWFLKEAFTLRRAVGTAAIVAGVM
                     ALRMG"
     gene            complement(2801..3019)
                     /locus_tag="VGA59_03075"
     CDS             complement(2801..3019)
                     /locus_tag="VGA59_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718687.1"
                     /translation="MEPKDTKDKDRKAPREEFEVAYYTKELGRSLQRELLQRRLTDLS
                     DGSITAASHGEEPSPESNYVSPREDRAG"
     gene            3265..5319
                     /gene="fusA"
                     /locus_tag="VGA59_03080"
     CDS             3265..5319
                     /gene="fusA"
                     /locus_tag="VGA59_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX9718688.1"
                     /translation="MPTTPAEIAALRTLALVGPATSGKTSLAEALLWKAGAIGAAGSV
                     ERGSTVSDHDPLEKRAQRSLNASIVHFKHRGVLAHVIDTPGAPDFLGQSLPPLEAVET
                     AAVVISATTGIEPMAVRMMQWAAQRERDRLIIVNKIDAQGVNLQALVAQIQATWGREC
                     LPVNLPAEGGARVVDCFYSMAKDVPATDFSSVEHAHRALVEQVVEVDAAFVERYLNDG
                     DVDPQELHAPLEQALREGHLIPICFVSARTGAGVPELLDVIARLLPDPTEANPPAFLL
                     GEGADATPLRAEPDPARHVLGHVFKITQDPYVGKMGVLRVHQGTLTTGSQLYVGDARR
                     PFKVGHLFMLQGKEFTEVQRALPGDICAIAKVEELHFDAVLHDSPDDDRIHLKPLDFP
                     VPVHGLAIEPKRRGDEQRLHDILQKLVSEDPCLRLEHVAVTNESVVYGLGELHLRTLL
                     DRLTEVHRCEVNTRPPRIAYRETITAPAEGHHRHKKQTGGAGQFGEVFLRIEPLPRGA
                     GFEFVDQVKGGAIPYNFMPAVEKGVRLALEAGVVAGYPVVDLRVIVYDGKHHTVDSKE
                     IAFIQAGKKAVQAAVQSARPSVLEPIVNVEIMAPEENIGDITGDLASRRGQVSGTTSA
                     TGALAVVAQAPLSELASYQSRLNALTGGQGRYTIEFSHYEPVPPTIQAQLASQYKVKD
                     EG"
     gene            5410..5886
                     /locus_tag="VGA59_03085"
     CDS             5410..5886
                     /locus_tag="VGA59_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9718689.1"
                     /translation="MKIEKDTVVTLRFKVAEANGKLIEESRDPMVYLHGGYENTLPKI
                     EAALDSREPGYEVTLQLKPEDAFGTRDESLVRTIPKSEFPPGVKVGGQLEGHTDEGAP
                     HVFHVMKIKGDKVLLDGNHPLAGRELRFILKVTGVRAATHEEVTHGHVHGAHGHHH"
     gene            5899..6477
                     /locus_tag="VGA59_03090"
     CDS             5899..6477
                     /locus_tag="VGA59_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX9718690.1"
                     /translation="MKFKMAPNSLFAVLLRSPWWISMLVAVLFVAAALALLPEQYRVL
                     GATGGLPFAAIGLIALWQQMRLPSARRSEEILGAVATMNWPEFSALLEEGFTKQGYRV
                     ERMQGAADFALSRQGRTTLVAARRWKAARIGEESLQALYGAAQSQGAGNCLYVTLGEL
                     SANAQLFAKRNNVQLMQRQALAYLLKDVTARR"
     gene            complement(6492..6995)
                     /locus_tag="VGA59_03095"
     CDS             complement(6492..6995)
                     /locus_tag="VGA59_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718691.1"
                     /translation="MNRGWLHVLLFALPALLVSGIAALALFGAAAGALWLFAFGDNPW
                     PQSAETALGLGFAGAWLSLWLGLLLLAFLRGKRLTAAGALPQRDVLAAAGATMLALAI
                     IVLHQWRVGNLGPASDGERCSTFCQRKGYSGSSMPPRNTGLATCGCVDSQGRESLTVP
                     IGETHPR"
     gene            complement(7075..8073)
                     /gene="gap"
                     /locus_tag="VGA59_03100"
     CDS             complement(7075..8073)
                     /gene="gap"
                     /locus_tag="VGA59_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX9718692.1"
                     /translation="MTIKIGINGFGRIGRMVFRAAVQNFKDIEVVAINDLLEPDYLAY
                     MLQYDSVHGRFKGEVRVDGSTLIVDGKKIRLTAVKDPAELKWGEVGADIVVEATGLFL
                     TKETGQKHITAGAKKVIMSAPSKDDTPMFVFGVNDKTYAGQAIISNASCTTNCLAPLA
                     KVLHDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VIPELNKKLTGMSFRVPTSNVSVVDLTVELLKDASYKEICAEFKAQSEGALKGILGYT
                     EDKVVATDFRGDPRTSIFDADAGIALDSTFVKLVSWYDNEWGYSNKILEMARVITK"
     gene            complement(8289..>9343)
                     /locus_tag="VGA59_03105"
     CDS             complement(8289..>9343)
                     /locus_tag="VGA59_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9718693.1"
                     /translation="DQVLHDHKRRMAEEKPRWATRKASQEALQVFTAAIPELIGGSAD
                     LTGSNLTNTKSTPPLRFDIAGDVKLADGPQAADAPRRIGRHINYGVREFGMAAVMNGI
                     TLHGGYIPYGGTFLTFSDYSRNAIRMAALMKKRVIHVFTHDSIGLGEDGPTHQSIEHA
                     ASLRLIPNLDVWRPCDTAETAVAWAVALQNANRPTALLLSRQNLPYAPKAGLEDISKG
                     AYVLAEAAEVGLARKPQAVIIATGSEVQLALKAQELLAAQQIAVRVVSMPSTTAFDRQ
                     SLAYKSDVLPAGVPRIAVEMGATDYWWKYGCAAVVGIDRYGESAPASVLFKHFGFTPE
                     NLADTVRAVLQRKVSA"
BASE COUNT         1544 a         3049 c         3116 g         1634 t
ORIGIN      
        1 accgacgcag gtcggtttgg agccgcaggt ctcggccccc ttgaggggga gagggcgaag
       61 cccgaggggg catcgccgaa ggcgcttcgg gggtggggtt tcattcagat gatctcgaag
      121 tatcgcttca gttcccagtc cgtgacgccg tcgagccact ggcgccattc ccactcgcgg
      181 gtggcggcga aatgttcaac gaaggtgtct cccaggaaat cccgcgcaat ggtcgagttg
      241 ctgaagttgc gggtggtttc gatcagggtg cgcggggccc gggggatgtt ttccgcaccc
      301 tcgttggttc cggtgatcgg cgcagtggtg agcttcagcc ctttctccac cccatgaagg
      361 ccggccgcga tcacggcggc catggcaagg tagggattga tgtccgcgcc cgggcagcgc
      421 gtctccagcc gggtgccttt cgggctgccg gcgatcacgc gaaaactcgc cgtgcggttg
      481 tccatgcccc aggtcggctt gaccggcgcc cagaagccat cgaccagccg cttgtagctg
      541 ttgatggtgg gccagaacat cggcgcgaat tccatcaggc aggctacctg cccggccagg
      601 tagctctcga acagcgggct catcttgcgc gagcttttcg tgtcgaaaaa caggttggtc
      661 ttgccgtccg tcaggctctg gtggatgtgg ccgctgcagc ccgggtactg ctggctccac
      721 ttggccatga agctgggcat gatgccgaag cgcacgccta tctccttggc cccggtcttg
      781 aacaggacgg cgcggtcggc ctgctccagc gcctccgaga acgagatcgc cgcctcacac
      841 gcgccggggc cggtctcggt gtgcagaccc tcgatcggca cgccgaaggc gagcatttcg
      901 tccatcagcg cattgaagaa ctcgcggttc tggctcacgc gcaacagcga gtagccgaac
      961 atgcccggcg tgagcgtctc gggcctcacg ccattcttgg ccgcccagct ctgcggcgtc
     1021 tcgcggaaat tgaaccactc gtactccatg cccgtcatga cctggaagcc gagtttttcc
     1081 gcgcgccgca ggacccgctt gagggtctgg cgcgggcaga tcggactggg actgccgtcg
     1141 gtattgatga attcgccgag gaagaaatcg acgccgccat cccagggaac ccggcggtgc
     1201 gtgtccaggt ccagccgggc cagggcgtcg gggaagccat gctgccagcc ggtgacctgc
     1261 gcgttgtcgt aggtgacgtc gccgcagtcc cagccgaaga ccacatcgca gaagccgaag
     1321 ccgccatcgg ccgcgccctc gaacttgtcc ttgtgcaggt acttgccgcg cagcacgccg
     1381 tcgatgtcgc tgcaggctac cttgaccttg tcggcgccgg atttgcggat cgcggcgagc
     1441 gcggggtgga gttcggttgc catcgggttg tcttcctctc agccgtgatc cagatcgcgg
     1501 gttcgcccgg ggcgatcccc atgttctcca gcgccagcgc caccgcctgc acggcctgct
     1561 ccatttcatt cgggccgatg gcgccgatac agcctacgcg aaatgtctcc agccgtgtga
     1621 gcttgccggg atacaggatg aaaccacact gccgcgccgg cgagtagaag gcctggaatt
     1681 cgtagcgcgg atcgctgtcc cacgaccccc agtcgcgcag catcgcgagc ttggtgctgc
     1741 tctaatggac accatgaatg cggcccttca tcccaccatc gcactgctgc agagcagctc
     1801 gctgaccccc gtggtgcagc aggagatcga gcgtgccatc ccgcagggcg agcacgcgcc
     1861 gggcagcaaa ttgatcgaag cgaacctggc cgggcagatg ggcgtctcgc gcgggatcaa
     1921 gctgcatgtg gaggcgaagc gctaccgatg acgctgacct ggccggtggt cgcggcggtg
     1981 ctgttcggcg cgctgctgca cgccggctgg aatgccctgg tcaagtccag cagcgacaag
     2041 gcgctggaca ccgccgtgat ccacctgctg ggctcgctgg tggcgctgcc catcgccctg
     2101 gtcgtgggct ggccgccggc ggcttcctgg cccttcatcc tgacttcggt ggtgatccac
     2161 atcggctact acatcgccct caccggcgcc tacaagcacg gcgacctggg cctgacctac
     2221 ccgctgatgc gcggcacggc gccgttgctg gtggcgctgt cggccgcggc gaccctgggc
     2281 gagacgctgt cgccgctgtc ctgggccggc gtgctgggcg tgagctgcgg cgtgctggcc
     2341 ctgggcctgt cgcgccacgc cctggattcg ccgcgcgcgg tcggctttgc gctggccaac
     2401 gcggtcgtga tcgccgtcta cacggtcgtc gacgcgctgg gcgtgcgcgc tagcggccat
     2461 gccctgcagt acgtggtcgc gctgttcgcg ctggatggct ggcccttcgc gctgatggtg
     2521 ttcgcgcgcc gcggcggggc cacggtctgg ccctatgccc gcgtccgctg gccggtggcg
     2581 agcctcgccg ccgcggcgtc gctgggttcg tacggcatcg cgctatgggc catgacccgc
     2641 gcgccggtcg ccaccgtggc cgcactgcgc gaaacgtcgg tgctgttcgc cgctttgctg
     2701 ggaacctggt tcctgaaaga ggccttcacc ttgcggcgcg cagtcggcac cgcggccatc
     2761 gtcgcgggcg tgatggcctt gcggatgggg tgacggcacc tcaaccggcc cggtcttccc
     2821 gaggcgagac gtagttcgac tccggggacg gctcctcgcc atggctggct gccgtgatgc
     2881 tgccgtcgct caaatcggtc aaccgccgct gcagaagctc gcgttgaagc gaccgtccca
     2941 gctctttcgt gtagtaggcc acctcgaatt cttcccgggg agcctttcga tctttgtcct
     3001 tcgtgtcctt cggttccatc aaggcagaat agcaagcgag cactcacgtg gtgtaggacg
     3061 gcatccatcc tggctaagga cggcagccta cggctgtaag agctcggata cattgggcag
     3121 ggcaggaaca agccctttcc tcgcttaaca ttgcacatac tttctgaccc tgaggagagg
     3181 ttatgagccc ccacgttcac cttcgttccg cgcccgccga ggccgcgggt gccgccccag
     3241 aggcggctcg tcgggaggga tgacatgcca acaacacctg cggaaatcgc cgcgcttcga
     3301 acattggccc tggtggggcc cgccacctcc ggcaagacca gcctcgcgga ggcactgcta
     3361 tggaaggccg gcgccatcgg cgcggccggc agcgtggagc gcggctccac ggtcagcgac
     3421 cacgaccccc tggaaaagcg cgcgcaacgg tcgctcaacg cctcgatcgt ccacttcaaa
     3481 cacaggggcg ttcttgcgca cgtgatcgac acgcccgggg cgcccgactt cctgggccag
     3541 tcgctgccac cgctggaggc ggtcgaaacg gcagctgtcg tgatcagcgc caccaccggc
     3601 atcgagccca tggccgttcg catgatgcag tgggcggccc agcgcgaacg cgaccgactg
     3661 atcatcgtga acaagatcga tgcccagggc gtgaacctgc aggcgctggt ggcgcagatc
     3721 caggcgactt ggggacgcga atgcctgccc gtcaacctgc cggccgaagg cggcgcgcgc
     3781 gtggtggatt gcttctacag catggcgaag gacgtccccg ccaccgactt ttcttcggtc
     3841 gagcacgcgc accgggccct cgtcgagcag gtcgtggagg tcgacgccgc cttcgtcgag
     3901 cgctacctca acgatggcga cgtcgatccg caagagctgc atgcgccgct ggagcaggcg
     3961 ctgcgcgaag gccacctgat cccgatctgc ttcgtgtccg cgcgcaccgg agcgggcgtt
     4021 cctgaattgc tggacgtgat cgcgcggctg ctgcccgatc cgaccgaagc caacccgccg
     4081 gccttcctgc tgggcgaggg cgccgacgcg acgccgctgc gcgccgagcc cgatccggcc
     4141 cgccacgtgc tgggccatgt cttcaagatc acccaggacc cttacgtggg caagatgggg
     4201 gtgctgcgcg tccaccaggg cacgctgacg acgggcagcc agctctacgt cggcgacgcg
     4261 cgccggccgt tcaaggtcgg gcatctgttc atgctgcagg gcaaggaatt cacggaggtg
     4321 cagcgcgcgc tgccgggcga catctgtgcg atcgccaagg tggaagagct gcacttcgac
     4381 gccgtgttgc acgactcgcc cgacgacgac cgcatccacc tcaagccgct ggacttcccg
     4441 gtgccggtgc acgggctggc gatcgagccc aagcggcggg gcgacgaaca gcggctgcac
     4501 gacatcctgc agaagctggt cagcgaagat ccgtgcctga ggctggagca tgtggccgtc
     4561 accaacgaaa gcgtggtgta cggcctgggc gagctgcacc tgcgcaccct gctggaccgg
     4621 ctgaccgagg tgcaccgctg cgaggtcaac acccggccgc cgcgcatcgc ttaccgcgaa
     4681 accatcacgg cgccggccga aggccatcac cggcacaaga aacagaccgg cggcgccgga
     4741 cagttcggcg aagtgttcct gcggatcgag ccgctgccgc gcggcgccgg cttcgagttc
     4801 gtggaccagg tcaagggcgg cgcgatcccg tacaacttca tgccggccgt ggaaaaaggt
     4861 gtgcgcctgg cgctggaggc gggcgtggtg gcgggctacc cggtggtgga cctgcgcgtg
     4921 atcgtgtatg acggcaagca ccacaccgtg gacagcaagg aaatcgcgtt catccaggcc
     4981 ggcaagaaag cggtgcaggc ggccgtgcag tcggcgcgac caagcgtgct ggagccgatc
     5041 gtgaacgtcg agatcatggc gccggaagag aacatcggcg acatcaccgg cgacctggcg
     5101 tcgcgccgcg gccaggtcag cgggaccact tcggccaccg gcgcattggc cgtggtggcg
     5161 caggcgccgc tctcggaact ggccagctac cagtcgcgcc tgaatgcgct gaccggcggc
     5221 cagggccgct acaccatcga gttcagccac tacgagccgg tgccgccgac gatacaggcg
     5281 caactggcgt cgcagtacaa ggtgaaggac gagggctgac gggcggagca acgacatgag
     5341 aagcggccct cgcggccgct ttttcttgcc ggttcgccgc ctcgataagc ttcgcggcag
     5401 aatcgcggca tgaaaatcga aaaagataca gtggtcacgc tgcgcttcaa ggttgccgaa
     5461 gccaacggca agctcatcga ggagagccgc gatcccatgg tttacctgca cggcggctac
     5521 gagaacacgc tgccgaagat cgaggccgcg ctggacagcc gcgagccggg ctacgaggtg
     5581 acgctgcagc tcaaaccgga agacgctttc ggcacccgcg acgaatcgct ggtgcgcacc
     5641 attcccaaga gtgagtttcc gcccggggtg aaggtgggcg gccagctgga aggccacacc
     5701 gacgagggcg cgccgcatgt cttccatgtc atgaagatca agggcgacaa ggtgttgctc
     5761 gacggcaatc atccgctggc gggcagggaa ctgcgcttca tcctgaaggt gaccggcgtg
     5821 cgggcggcga cccacgaaga ggtcacgcac ggccacgtgc acggggcgca cggtcatcat
     5881 cattgagcgg aagctgccat gaagttcaag atggctccca attccctgtt cgcggtgctg
     5941 ctgcgctcac cgtggtggat cagcatgctg gtcgccgtat tgttcgtggc ggcggctttg
     6001 gcgttgctgc cggagcaata ccgcgtgttg ggcgccacgg gcggcttgcc gtttgcggcc
     6061 attggcctga tcgccctttg gcagcaaatg cgcctgccca gcgcccgccg gtcagaggaa
     6121 atactcggcg cggtggccac catgaactgg ccggagtttt ccgccctgct ggaagaggga
     6181 ttcacgaaac agggctatcg cgtcgaacgc atgcagggcg cggccgactt cgcgctctcg
     6241 cgccagggcc gtaccaccct ggtcgccgcc cggcgctgga aggctgcgcg catcggcgag
     6301 gagtcactgc aagcgcttta tggcgccgcg caatcgcaag gcgcgggcaa ttgcctctac
     6361 gtaactctgg gagagctgag tgctaatgcg cagctatttg ccaagcgcaa caatgttcag
     6421 ctcatgcagc ggcaggcgct ggcctacctg ttgaaggacg tgacagcccg acgctgacgc
     6481 gatgccccgc ttcaacgagg gtgcgtctcg ccgatcggca cggtgagcga ttcgcgtccc
     6541 tggctgtcca cgcagccgca ggtcgccagt cccgtattcc tcggcggcat gctgctgccg
     6601 gagtacccct tgcgctggca gaaggtgctg catcgttcgc cgtcggaagc cggaccgagg
     6661 tttcccacgc gccattgatg cagcacgatg atggccagag cgagcatcgt cgcaccggcc
     6721 gctgcgagca catcgcgttg cggcagcgcg ccggcggcgg tcagtctctt gcctctgagg
     6781 aacgccagca ggagcaggcc gagccagagc gacagccagg cgccggcgaa cccaaggcca
     6841 agcgcagttt ccgcggattg cggccacggg ttgtcgccga atgcgaacag ccacaacgcc
     6901 ccggccgccg cgccgaacag ggcgagcgcg gcaatgcccg agaccagcag cgccggaagg
     6961 gcgaacagca gaacatggag ccagccgcgg ttcatgcccg atctttgccc aagccctgcc
     7021 gcaaagcaac ggccaaaaaa acgcgcctga cggcgcgttt ttcttgagca agccttactt
     7081 cgtgatcacc cgcgccatct ccagaatctt gttggagtag ccccattcgt tgtcgtacca
     7141 ggacaccagc ttgacgaaag tgctgtccag ggcgatgccg gcgtcggcgt cgaagatcga
     7201 ggtgcgcgga tcgccgcgga agtcggtggc caccaccttg tcctcggtgt agcccaggat
     7261 gcccttgagc gcgccttcgc tttgcgcctt gaactcggca cagatttcct tgtagctggc
     7321 atccttgagc agttccaccg tcaggtcgac caccgacacg ttcgacgtgg gcacccggaa
     7381 gctcatgccg gtgagtttct tgttcagctc ggggatcacc acgcccacgg ccttggccgc
     7441 gccggtgctg gacggaatga tgttttccag gatgccgcgg ccgccgcgcc aatccttgtt
     7501 gctcgggccg tccaccgtct tctgggtggc ggtggcggcg tgcaccgtgg tcatcaagcc
     7561 gcgcttgatg ccccacttgt cgtgcagcac cttggccagc ggcgccaggc agttggtggt
     7621 gcaggacgcg ttggagatga tcgcctggcc ggcataggtt ttgtcgttca cgccgaacac
     7681 gaacatcggc gtgtcgtcct tcgagggcgc cgacatgatg accttcttcg cgccggcagt
     7741 aatgtgcttc tggccggttt ccttggtcag gaacaggccc gtggcctcga cgacgatgtc
     7801 ggcaccgacc tcgccccact tgagttccgc cgggtccttc acggccgtga gccggatctt
     7861 cttgccgtcg acgatcaggg tactgccgtc gacgcgcacc tcacccttga agcggccatg
     7921 cacgctgtcg tactgcagca tgtaggccag gtagtcgggc tcgagcaggt cgttgatggc
     7981 gacaacctcg atgtccttga aattctgcac ggcggcgcga aacaccatgc gcccgatgcg
     8041 gccgaaaccg ttgatgccga tcttgatggt cattggggac tcctgatagt gaagaatttg
     8101 aaacggatgt cattgcgggc ccgatccgca atccatgttg gagagtcgtg acgcaacttc
     8161 attcgatacc tggatcccgg gtcaagcccg ggatgacgcc tttggcgcga cggatgacag
     8221 gactttgcga acagtatcca ccacattgtg gctccggcat aacaagtgca ttctgcacag
     8281 ttgttagcct atgccgaaac cttgcgttgc aataccgcgc ggacggtatc ggcaaggttt
     8341 tccggcgtga aaccgaagtg cttgaacagc acactggccg gcgcggactc gccatacctg
     8401 tcgatgccga ccacggccgc gcagccgtat ttccaccagt agtcggtcgc ccccatctcc
     8461 acggcaatgc gcggcacgcc cgcgggcagg acatcgctct tgtaggcaag gctctgccgg
     8521 tcgaaagcgg tcgtgctggg catggacacc acacgtactg cgatctgctg cgccgccagc
     8581 agttcctggg ccttgagcgc cagctgcacc tccgagccgg tggcgatgat cacggcctgc
     8641 ggcttcctgg ccaatcccac ctcggccgcc tcggccagca cataggcccc cttgctgatg
     8701 tcttccagcc cggccttggg cgcatagggc aggttctggc gcgacagcaa cagcgccgtg
     8761 ggccggttcg cgttctgcaa cgcgacggcc caggcgactg ccgtctcggc cgtatcgcac
     8821 gggcgccaga cgtccaggtt ggggatcagg cgcaggctgg cggcgtgctc gatcgactgg
     8881 tgggtggggc cgtcctcgcc caggccgatg gagtcatgcg tgaacacatg gatcacgcgc
     8941 tttttcatga gcgcggccat gcgaatcgca ttgcggctgt agtcgctgaa cgtgaggaag
     9001 gtgccgccgt aagggatata gccgccgtgc agggtgatgc cgttcatcac ggcggccatg
     9061 ccgaactcgc gcacgccgta gttgatgtgg cggccgatgc ggcgcggcgc gtcggctgct
     9121 tgcgggccgt ccgccagctt gacgtcgccg gcgatgtcga agcgcaacgg gggcgtgctc
     9181 ttggtattgg tgaggttgga gccggtcagg tcggccgagc cgccgatcag ctccgggatc
     9241 gccgcggtga acacctgcag cgcttcttgg ctcgccttgc gggtcgccca gcgcggcttt
     9301 tcctccgcca tgcgccgctt gtggtcgtgc aggacctggt cca
//
LOCUS       DASWIY010000081         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1159323, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000081 DASWIY010000000
VERSION     DASWIY010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159323"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VGA59_03110"
     CDS             <1..956
                     /locus_tag="VGA59_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX9718694.1"
                     /translation="DQGGELLYLARAERLLLDGERVSGIECLGMDERSVAPNGRRIRV
                     KARHYVLAGGGINTPGILLRSKAPDPHRRTGRRTYLHLVNFSAAQFEQVINPFYGAPQ
                     SIYSDHFQWDDGVTGRLSYKLEVPPLQPALAATLLGRFGIDNALRMEQLPNTNVMLAL
                     LRDGFHPDSAEGRVELRGDGSPVLDYQMTDYSWDGVRRAFHSMAEIQFAAGAGAVLPL
                     HTDAGYVKTLGEARRLIDSLSLEIYRTRLGSAHVMGGCAMGENPRQAVCDSLGRHHQL
                     DNLSIHGGSLFPTSIGANPQLSIYGLTAQLASQLAERLKHA"
     gene            1068..1547
                     /gene="coaD"
                     /locus_tag="VGA59_03115"
     CDS             1068..1547
                     /gene="coaD"
                     /locus_tag="VGA59_03115"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX9718695.1"
                     /translation="MNRVLYPGTFDPITKGHGDLVERAARLFDSVIIAVAASPKKNPL
                     FPLEQRVALAREVTKHLPNVEVMGFSTLLAHFAQEQGANVLLRGLRAVSDFEYEFQLA
                     NMNRQLAPDVESLFLTPSEKYSYISSTLVREIAALGGDIGKFVHPAVADALHERFKR"
     gene            1761..>2001
                     /locus_tag="VGA59_03120"
     CDS             1761..>2001
                     /locus_tag="VGA59_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEX9718696.1"
                     /translation="MSLIITDDCINCDVCEPECPNGAISQGEEIYVIDPNLCTECVGH
                     YDEPQCQQVCPVDCIPLDANHVESKDELMEKYLIIT"
BASE COUNT          371 a          701 c          597 g          332 t
ORIGIN      
        1 tggaccaggg cggcgagctg ctctacctgg cccgcgccga acgcctgctg ctcgacggcg
       61 agcgggtcag cggcatcgag tgcctgggca tggacgaacg cagcgtcgcg cccaacggcc
      121 gcaggatccg ggtcaaggcc aggcattacg tgctggccgg cggcggcatc aacaccccgg
      181 gcatcctcct gcgctcgaag gcgccggacc cgcaccggcg caccggcagg cgcacctacc
      241 tgcacctggt gaacttctcc gccgcgcagt tcgagcaggt gatcaacccc ttctacggcg
      301 cgccgcagtc gatctattcc gatcacttcc agtgggacga cggcgtcacc ggacgcctgt
      361 cctacaagct ggaagtgccg ccgctgcaac cggcgctggc cgccaccctg ctcggccgct
      421 tcggcatcga caatgccctg cgcatggaac agctgcccaa caccaatgtg atgctggcgc
      481 tgctgcgcga cggcttccac ccggacagcg ccgaaggccg cgtggaactg cgcggcgacg
      541 gcagcccggt actcgactac cagatgaccg attacagctg ggatggcgtg cgccgcgcct
      601 tccacagcat ggccgagatc cagttcgccg ccggagccgg ggcggtgttg ccgctgcata
      661 ccgacgccgg ctacgtgaag actctgggtg aagccaggcg cctgatcgac agcctgagcc
      721 tggaaatcta ccgcacccgc ctgggcagcg cccacgtgat gggcggttgc gccatgggcg
      781 aaaacccgcg ccaggcggtc tgcgacagcc tcggccgcca tcatcagctg gataacctgt
      841 cgatccacgg cggctcgctg ttccccacca gcatcggcgc caacccgcaa ttgtcgatct
      901 atggcctgac cgcgcaactg gcgagccaac tggccgagcg cctcaagcac gcctgatgga
      961 agggtatgca cgagccccgc cgggctcgtg cggcacacgc ttgacttggc cgcagttcat
     1021 cgctgcgcta ccatccgact ccccaacgga tccgccagga cgtcacgatg aatcgagtgt
     1081 tataccccgg caccttcgac cccatcacca aaggccatgg cgatctggtc gaacgcgccg
     1141 cgcgcctgtt cgacagcgtg atcatcgcgg tggccgccag cccgaagaag aacccgctgt
     1201 tccccctcga gcaacgggtc gcactggccc gcgaagtgac caagcacctg ccgaatgtcg
     1261 aggtgatggg cttctccacc ttgctcgcgc acttcgccca ggagcagggc gccaatgtgc
     1321 ttctgcgcgg cctgcgcgcg gtctccgact tcgaatacga gttccagctg gccaacatga
     1381 accgccaact ggcgccggac gtggagagcc tgtttctcac tccgtcggaa aaatactcct
     1441 atatttcctc gaccctggtg cgcgagatcg ccgcgctggg tggtgatatc ggcaaattcg
     1501 tccatcctgc cgtggccgac gccctgcacg agcgtttcaa gcgctgagcg accacccttg
     1561 taggagcacg ccatgcatgc gatcggtatc gcgggcagcg ccggttcgca ggcatggcct
     1621 gctcctacag cccagatgac ctgttcccga cccgcccaca catggcgccc gcttcaacaa
     1681 tccggcacaa ttcgtccatt gttgtctacc tattgtttga aaatgtcgta gccgaaggct
     1741 gcgacaggag cgtttagtcc atgtccctga tcatcaccga cgactgcatc aactgcgacg
     1801 tctgcgagcc cgagtgtccg aacggcgcga tctcccaggg cgaggagatc tacgtgatcg
     1861 accccaacct gtgcaccgaa tgcgtcggcc actacgacga gccgcagtgc cagcaagtct
     1921 gccccgtgga ttgcatcccg ctcgatgcga accatgtcga gagcaaggac gaactgatgg
     1981 agaagtacct gatcatcacc g
//
LOCUS       DASWIY010000082        46693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1906025, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000082 DASWIY010000000
VERSION     DASWIY010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 46693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46693
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906025"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..446)
                     /locus_tag="VGA59_03125"
     CDS             complement(195..446)
                     /locus_tag="VGA59_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HEX9718697.1"
                     /translation="MIEITLAADAWKDIDPGTEALVDKWLVGEGDTVRAGQPIANVVI
                     VKTNQEIVAPADGRIEKILVKSEETFARGKPIALLKEST"
     gene            complement(443..1483)
                     /locus_tag="VGA59_03130"
     CDS             complement(443..1483)
                     /locus_tag="VGA59_03130"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX9718698.1"
                     /translation="MTATRKLTMAQAISEGIAQEMERDPNVFVMGEDIGKYGGIFGAT
                     GGLLDKFGKDRIMDTPISETAFMGTATGAAAAGLRPIVELMFVDFFGVCMDQIYNHLA
                     KNTYMAGGNIRLPVVLTTAIGGGYNDAAQHSQCLYATFAHMPGIKVVVPSNAYDAKGL
                     MIQAIRDDNPVMFMYHKGIMGLPWMAYFEGSSNEVPEAAYTIEFGKAKVAREGKDVTI
                     VTISQMVQKALIAAAELARDGVSAEVLDLRTLVPLDKAAVLESVAKTGRLLIADEDYL
                     SFGLSGEISAVVAENLDTLTLKAPIKRLAVPNVPIPYSRPLEQFVIPQVGSIVDAVQK
                     VMKARVAERLAA"
     gene            complement(1514..2521)
                     /locus_tag="VGA59_03135"
     CDS             complement(1514..2521)
                     /locus_tag="VGA59_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX9718699.1"
                     /translation="MQANKEKLLWMYETMLEIRDYEETMANVYLEGKLPPHIQKGLAF
                     DIGSGPVPGEMHLAAGQEPVAVGVCAHLKKEDTVVGTHRPHHFAIAKGVPLDKMTAEI
                     FGKVTGLGKGKGGHMHLFDPEHRFSCSGIIGASLPPACGAALAAKKMGKDWVAVAFFG
                     EGAANQGSFHESLNLAALWQLPVIFVCEDNKWAISVPKSESTCVEWVADRASAYGIPG
                     VLVPNNDALEVYEAVAPLIERARGGAGPGLIEVQTDRYLGHFQGDPETYRPKDEVADL
                     RKNDPIPKLAGVLKSLGMMTEGEQGAILARAKKRVADAFAFARESAYPQAQAALDDVF
                     V"
     gene            complement(2690..3820)
                     /locus_tag="VGA59_03140"
     CDS             complement(2690..3820)
                     /locus_tag="VGA59_03140"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin dehydrogenase
                     dihydrolipoyllysine-residue acetyltransferase subunit"
                     /protein_id="HEX9718700.1"
                     /translation="MDDSARAGITPIVMPKWGLSMKEGTVMSWLVEEGAEISVGAPIL
                     EVETDKIANAVEAPDPGTLRRKVAQDGETLPVKALLGVMAGPGVSDADIDAYVAAYIT
                     PAESEGEDAEAAPAYQFADVDGLRIRYARKGAGSGVPVLFIHGFGGDLDNWLFNLDAV
                     GEKSPVIALDLPGHGQSTVRLPGTGIGALAGFVARFMDVLGVEQAHLVGHSMGAAIAA
                     QMALDHPRRVASVALIAAAGLGPEINAGFTEDFVAAPSRRELKRVLEQLFAKPELVRR
                     QMVDDVLKYKRLDGVPELLGALSRDMFGGDRQAEQPGLRLEETGKPVLVIWGREDRII
                     PAHHAGSAPPGATVEVLDGAGHMVQMERANDVNRLLKHHVGV"
     gene            complement(3820..4836)
                     /locus_tag="VGA59_03145"
     CDS             complement(3820..4836)
                     /locus_tag="VGA59_03145"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX9718701.1"
                     /translation="MARKISMKQAINEALDQEMARDPNVICMGEDIVGGAGGDGEMDS
                     WGGVLGVTKGLYAKHGDRLMDTPLSESAYIGAAIGAAACGMRPVAELMFIDFMGVCFD
                     QIYNQAAKFRYMFGGKAETPVVIRAMVGAGFRAAAQHSQMLTPVFTHIPGLKVVCPST
                     PYDTKGLLIQSIRDNDPVIFCEHKNLYGFEGEVPEASYTIPFGEANVVREGRHCSIVT
                     YGLMVHRALEAAATLAKEGVEVEIVDLRTLSPLDLDTVLESVTKTGRLVCVDEANPRC
                     SIAADISAQVAMQAFGALKAAIEMVTPPHAPVPFSPTLEDLYIPSGAQVADAVRKTLK
                     GGKH"
     gene            complement(4852..5838)
                     /locus_tag="VGA59_03150"
     CDS             complement(4852..5838)
                     /locus_tag="VGA59_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX9718702.1"
                     /translation="MAGKNPFPLTRDELLKCYRTMRTIREFEERLHIDFGRGDIPGFV
                     HLYAGEEASAAGIMIHLGDGDRIASTHRGHGHCISKGVDVVAMMKEIYGRKGGACNGK
                     GGSMHIADLSKGMMGANGIVGAGAPLACGAALAAKFRGKGEVAISFVGDGASNQGTFL
                     ESLNLAAMWNLPAIFVVENNGYAETTSRDYAVAVDSYVDRAAGFGLPGITVDGTDFFA
                     VHEAAGEIIKRARAGGGPALLECKMVRFYGHFEGDAQTYRGPGELEDIRANKDCIKKF
                     AAAVTGAGVVSAAELKAIGDEVLAVIEDAVKQAKAAPLPTAADLTTDVYVTY"
     gene            complement(5853..6908)
                     /locus_tag="VGA59_03155"
     CDS             complement(5853..6908)
                     /locus_tag="VGA59_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX9718703.1"
                     /translation="MQADHSNATPCIGVIANPVSARDIRRIVAYAGNLQIADRVNIVL
                     SVLTVAGAMGVRRVLMMPDNGGIRAMLMRSLKREGNLHHAFPEVEFLDMDPTSTVDDT
                     FLAARMMHAAGVKAIIVLGGDGTHRAAVRECADTPIAGLSTGTNNAFPEMRAPTVTAM
                     AVGLYATGKLSAEQALAPNKLLEVSINEGQRHDIALVDAVISTDRYIGARALWKTESL
                     AAIYLAFADPQAIGLSAIGGLLHPLGRREAGGLALQLGGDPATRRLALRAPIAPGMLR
                     EVGIAHWEPMPADQPFPVALQAGVVALDGEREMAFSAGDHVRVTLRENAFTTVDVARC
                     MHTAAREGLFRLSSSTL"
     gene            complement(7201..9141)
                     /locus_tag="VGA59_03160"
     CDS             complement(7201..9141)
                     /locus_tag="VGA59_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX9718704.1"
                     /translation="MQLQQREHIATVMQVAGDDILAPETLAHDEIIRASWQRCVHEHR
                     LDPTRPREAVILPGERLREHQDQMEEFLQIARHGLETLYRKVAGMGYAVLLTDARGVT
                     VDFIGDLQFDHSLRRAGLYLGADWNEVSAGTCGVGTCISTGHALTVHQGDHFDATHIA
                     LTCTAAPVYGAQGQLKAVLDISALTSPLPKASQHLALQLVKVYAHHVENASFLHQFRH
                     DWVLRLSAAPQFLDMNPEYLLAVDATGRVVGHNRRAQLLLEADGHAPILGRPFAQLFN
                     ASFDDLGRFVQVRPSDQRAITLAGSARLLFMLASPPSARWTPPPGQPSERKVPAALAA
                     LSGGDPLLERQIERAARLINSPVSMLLTGETGTGKEYFAKALHESSERRSRPFVAVNC
                     AAIPETLIESELFGHLSGSFSGAAPKGKRGLIQEADGGTLFLDEIGDMPRALQPRLLR
                     VLAEHEVLPVGATQPVAVNIRVIAATHWDLEALVREGRFRDDLYYRLNGARIALAPLR
                     ERRDLDWMIDRMLRQGFTGEPPQLAPEVRAWMRAHRWPGNLRELRNVIEYARAVCSDG
                     YIRLGDLPDELGAAMAPRAARPHHAKPGAEALLLMQYLRAASWNVTAVARQLGVSRMT
                     LYRRMKRFGIVSPNHLDDAPPQ"
     gene            9939..10547
                     /locus_tag="VGA59_03165"
     CDS             9939..10547
                     /locus_tag="VGA59_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9718705.1"
                     /translation="MRNHACRLVAAIGLTLLSPSAFSALEDAQSIFGKAVMYAFFLFV
                     IVAAAVVYFMRFQDKRRTPLHKVFEQGEAIHSVRPDALVSECASRMTSEKIGALVVMD
                     GERLIGIFTERDALSRVLAQGLDPGRTKISDVMTRDPYCIPPTTTVGEAMRLVTTRRF
                     RHLPIVENNKVLAVISSGDLTHWLVKDQIGEVQELVDLAARS"
     gene            10740..10985
                     /locus_tag="VGA59_03170"
     CDS             10740..10985
                     /locus_tag="VGA59_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718706.1"
                     /translation="MNEINEKVSMWAKLHKRWEKVESDLRSVKTLPAREVGVNISAME
                     VELRALRAKVDTAFNEASGALRDKSAAKTRDDHRELL"
     gene            11193..11939
                     /gene="aat"
                     /locus_tag="VGA59_03175"
     CDS             11193..11939
                     /gene="aat"
                     /locus_tag="VGA59_03175"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX9718707.1"
                     /translation="MNLPWLDPGEPFPEASEAWDTSQPAPGLLAAGGALDVDSLHRAY
                     SAGIFPWFSEGQPILWWSTDPRMVLFTREFKLHRSLRKTLARFVADPRCQLRIDTNFA
                     EVIHACSLSERPGQSGTWIVPQMVKAYEAFHRAGFAHSVETWVGGRLMGGLYCVAIGR
                     AVFGESMFTRMPDASKIALAGLTAFCRHHGIAMIDCQQNTQHLASLGAREIPRADFVA
                     QVSQNTKKAPAPWRFDPVYWNELLPPNPSA"
     gene            11936..12679
                     /locus_tag="VGA59_03180"
     CDS             11936..12679
                     /locus_tag="VGA59_03180"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX9718708.1"
                     /translation="MTHLKDLPLQTLQFYATAPYPCSYLPGRQARSQVATPSHLIHND
                     AYSDLVVNGFRRSGMFTYRPYCDGCRACVPLRVLSQQFRPDRSQRRAWVRHQNLQARV
                     LKLCFVPEHYHLYLRYQTGRHAGGGMDHDSIDQYTQFLLQSRVNSRLVEFRETRADGS
                     AGALRMVSILDVLNDGISAVYTFYEPEGAASYGTYSVLWQIEQARRMRLPHVYLGYWI
                     GQSPKMNYKSRFIPHEILVEGHWIRGTGG"
     gene            12758..13555
                     /locus_tag="VGA59_03185"
     CDS             12758..13555
                     /locus_tag="VGA59_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9718709.1"
                     /translation="MRKIDPGAHATPTIQVIERMFLLIDVLASHEDSVSLKEISEKTG
                     LHPSTTHRILNDLATGRFVDRPEAGSYRLGMRLLELGNLVKGRLNVRDAALTPMRELH
                     KLIQQPVNLSMRQGDEIVYIERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLSADDPQ
                     RVRAYATRTGLAGHTKNSITQLPILERELSKARQYGIARDNEELELGVRCMAAGIYDD
                     QSKLIAGLSISAPADRLDEGWLTKLQATANEISAALGFKTPPQRAAA"
     gene            complement(13631..14686)
                     /gene="pbpG"
                     /locus_tag="VGA59_03190"
     CDS             complement(13631..14686)
                     /gene="pbpG"
                     /locus_tag="VGA59_03190"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEX9718710.1"
                     /translation="MHHKAPSVMSKVLNRLLHSVAVAALVIGLAPAVADAAPRKAKAS
                     SVKKIHSSAGKRVVRSFAGKRKSVMRMAIVPARPSYGKLAGLHSVQDPLDLKSSVALV
                     IDQDTSEVLFSKNDSAVLPIASLTKLMTGLIISDAKLPMDEAIEITQDDVDTEKGSRS
                     RLRVGSKLTRGELLHLALMSSENRAAHALGRTYPGGMAAFVSVMNAKAQMLGMKDTRY
                     VEPTGLSSQNQSSAHDLATLVNTTYEDPVLREYSTSPGHQVAVGKRTLNFNNTNRLVK
                     NPDWDIGLQKTGYISEAGQCLVLQAKIAGRKLIMVFLDSAGKLSRIGDAERVRRWVES
                     NPLVPPAANAVFHQVKN"
     gene            complement(14802..16340)
                     /locus_tag="VGA59_03195"
     CDS             complement(14802..16340)
                     /locus_tag="VGA59_03195"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX9718711.1"
                     /translation="MADKLIIFDTTLRDGEQSPGASMTKDEKLRIARQLERLKVDVIE
                     AGFAASSNGDFEAVQAIAKGIRESTICSLSRANDRDISRAAEALKEAQRARIHTFIAT
                     SPLHMEKKLRMTPEQVLEQAKQSVRFARNLCDDIEFSPEDGYRSEMDFLCRVLEVAIA
                     EGATTINVPDTVGYAVPELYGLFIKTLRERIPNSDKAIWSVHCHNDLGMAVANSLAGV
                     KIGGARQVECTINGLGERAGNCSLEEVVMAVKTRRDYFGLDVGIDTRHIVAASRMVSQ
                     TTGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVGWSANKIVLGKLSG
                     RNAFKQRLQELGVALDSEADVNTAFTRFKELADRKSEIFDEDILALVSDESVTHEQEQ
                     YHFVSLAQHSETGERPHAVIVFTVEGKEVRGESDGNGPVDASLKAIESHVRSGAEMVL
                     YSVNAISGSTESQGEVTVRLQNSGRVVNGVGADPDIVVASAKAYLSALNKLQNKSDRV
                     AAQG"
     gene            complement(16403..18097)
                     /gene="leuA"
                     /locus_tag="VGA59_03200"
     CDS             complement(16403..18097)
                     /gene="leuA"
                     /locus_tag="VGA59_03200"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX9718712.1"
                     /translation="MLKNPASKYRAFAPVRLTDRTWPDAVLTRAPIWCSVDLRDGNQA
                     LIEPMDVARKLRLFEMLVDIGFKEIEVGFPSASQTEFDFIRKLIEDDLIPADVTIQVL
                     TQAREPLIRRTFESLRGAKSVIVHLYNATAPVMRKVVLGLDEDGIVELATSRARLFRE
                     LAAQQPQTRWTFEYSPEMFSGTELAFSKRVVDAVTEVWQPTPQARCIVNLPSTVEHST
                     PNIFADMVEWMHRNLARREAIVLSVHPHNDRGTGTAAGEFAVMAGADRLEGCLFGNGE
                     RTGNLDLVNVALNLYSQGIAPGLDFSDIDEIRRTVEHCSQMPIHPRHPYVGDLVYTSF
                     SGSHQDAIKKAFAGRKADGDVWDMPYLPLDPKDLGRSYEAVIRVNSQSGKGGISYLLE
                     SEYGLELPRRLQIEFSRVVQAVMDESGRELTARDLFELFEREYGIRSIAAPQHLVLEE
                     AQGRDGKLVKLAAEVELSGRRLNIQGEGNGPIDAFVQALASACGETVRVLDYHEHAIG
                     AGANARAVAYLELRIGARTLFGVGMDSNIVSASFKAIVSGLGRTRAGSTSEAALSS"
     gene            complement(18300..19118)
                     /gene="pssA"
                     /locus_tag="VGA59_03205"
     CDS             complement(18300..19118)
                     /gene="pssA"
                     /locus_tag="VGA59_03205"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX9718713.1"
                     /translation="MHDGNDSQSAPFTEGVVVRKRRKGIYILPNLFTLAALFGGFYAV
                     VMAMNGRFDLAAVGVFSAMVLDSLDGRVARMTNTQSAFGEQMDSLSDMVSFGAAPALI
                     AYEWGLKSLGRWGWIAAFVYCACAALRLARFNVNTGVVDKRYFQGLPSPAAAALVAGF
                     IWVMNDLEVKGSEVAWPMFACALYAGLTMVTNVPFYSFKDVQMKKSVPFVVIVLIALG
                     IAVINIHPPIVLFGLFVVYGLSGYVLYGWRKAKGQQASVISISTEEPDERGLHK"
     gene            complement(19230..20246)
                     /gene="ilvC"
                     /locus_tag="VGA59_03210"
     CDS             complement(19230..20246)
                     /gene="ilvC"
                     /locus_tag="VGA59_03210"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX9718714.1"
                     /translation="MKVYYDKDCDLSLIKGKTVAIIGYGSQGHAHAQNLNDSGVKVVV
                     GLRKGGASWGKVEKAGLKVAEVAEAVRSADVVMILLPDEQIAAVYQKDIEPHIRQGAS
                     LVFAHGFNVHYGFVTPRADLDVWMVAPKAPGHTVRHTYTQGGGVPQLVAVHQDKSGRA
                     RDLALSYAMANGGGKAGIIETSFREETETDLFGEQAVLCGGTVELIKAGFETLVEAGY
                     APEMAYFECLHELKLIVDMIYEGGIANMNYSISNNAEYGEYVTGPKIITDATKQAMKQ
                     CLKDIQSGEYAKSFILENRAGAPVLLSRRRLTAEHQIETVGETLRAMMPWIKKNKLVD
                     QSRN"
     gene            complement(20286..20777)
                     /gene="ilvN"
                     /locus_tag="VGA59_03215"
     CDS             complement(20286..20777)
                     /gene="ilvN"
                     /locus_tag="VGA59_03215"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX9718715.1"
                     /translation="MKHIIAVLLENEPGALSRVVGLFSARGYNIESLTVAPTEDPSLS
                     RMTIQTTGSDDVIEQITKHLNRLIEVVKVVDLTEGAYTERELMMVKVRAVGKEREEMK
                     RMADIFRGRIIDVTEKSYTIELTGNQGKNDAFLEAIDRSAILETVRTGSSGIGRGERV
                     LRV"
     gene            complement(20938..22740)
                     /locus_tag="VGA59_03220"
     CDS             complement(20938..22740)
                     /locus_tag="VGA59_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HEX9718716.1"
                     /translation="MEISKAEIASAAAANAASAGTSTTPGQSAELRGAEILVKALQAE
                     NVKFVWGYPGGAVLHIYDAFYKQDTIQHVLVRHEQAAVHAADGYARATGDVGVALVTS
                     GPGVTNAVTGIATAYMDSIPMVIITGQVPTHAIGMDAFQECDAVGITRPVVKHNFLVK
                     DVRQMAEVMRKAFHIARSGRPGPVVVDIPKDVSFNKTAYTGYTDKVEMRSYNPVRKGH
                     GGQIRKALQLLLAARRPYIYTGGGVVLGNATAELRTLVDMLGYPCTNTLMGLGSYPAS
                     DRKFLGMLGMHGTVEANNAMQNCDVLLAVGARFDDRVIGNPKHFGQNERKIIHVDIDP
                     SSISKRVRVDIPIVGDVKEVLGELVAMLKESGTRPDADALGAWWDTIEGWRKRDCLRY
                     DRKNTEVIKPQAVIETLWNMTKDADVYITSDVGQHQMWVAQYYRFNEPRRWINSGGLG
                     TMGVGIPYAMGIKMAKPASEVYCVTGDGSVQMCIQELSTCLQYNTPIKIISLNNRYLG
                     MVRQWQEIDYEGRYSHSYMDALPNFVKLAEAYGHVGMLIEHPKDVEPALREARKLKDR
                     TVFMDFRTDPTENVFPMVKAGKGITEMLLGSEDL"
     gene            23026..23592
                     /locus_tag="VGA59_03225"
     CDS             23026..23592
                     /locus_tag="VGA59_03225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9718717.1"
                     /translation="MATERELSDFLKSVEKRAFKRSIYHVRDEEAALDIVQDSMMKLA
                     EHYGDKPPAELPMLFQRILSNCTLDWFRRQKTRNALFSNMSDFESSTDDGDFDLLETY
                     AFINDSQQVESAEDATRRGQILREIESQIQELPVRQREAFLMRYWEDMDVAETAAAMG
                     CSEGSVKTHCSRAVQALSKALKAKGIQL"
     gene            23589..24017
                     /locus_tag="VGA59_03230"
     CDS             23589..24017
                     /locus_tag="VGA59_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HEX9718718.1"
                     /translation="MNDKALIDPQQYQDRFGRQVAARLSAGMAELPYGITERLRAARA
                     QAVARRKPASLRRAFSIFASGGAATLTFGEDGASLWNRVAAVLPLVVLAAGLVLIHAI
                     QDERRASEVAEVDAALLTDDLPPSAYADPGFAQFLRFSKE"
     gene            24130..24786
                     /locus_tag="VGA59_03235"
     CDS             24130..24786
                     /locus_tag="VGA59_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEX9718719.1"
                     /translation="MPATVLAQPQPQAVGAASAAASAPLPAAGTAASRAGSPRATASA
                     ARTNNNKRVTKPLWNELTPAQQQALAPLSGKWNTVSEAQKRKWLALSQNFPGMSGAEQ
                     AKLHSRMSEWAALTPQQRTQARLNFGETKQLSPDDKKAKWEAYQALPPEEKRKLAASA
                     PKPPATAAAVKPVPPEKLADVPKKPAAKTPRIATSPGQVDHNTLLPQPSPKRGDGRAN
                     "
     gene            24861..25343
                     /locus_tag="VGA59_03240"
     CDS             24861..25343
                     /locus_tag="VGA59_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX9718720.1"
                     /translation="MAPGLARRMACWLYEGILLFAVVFLAGWLFSTLGQVRDAMDARR
                     PLLQAFLFVVFAIYFSWFWARGQTLAMKTWNIRIVDRTGRPVSQLRALLRYAWGWLWF
                     LPPLALVATLKPGGGTSAVVVLGWVAVWALLSRFHPQRQFWHDEFAGTRLVSSVPLSR
                     "
     gene            25371..25784
                     /locus_tag="VGA59_03245"
     CDS             25371..25784
                     /locus_tag="VGA59_03245"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX9718721.1"
                     /translation="MRAMSSPPDALTPANQQKLRRGLSRVWHAAGYSLAGLRAGWGET
                     AFRQEAVASVALLPVAFWLGRTWVEAALLAGSVLIVMIVELLNTGIETAIDRIGPEWH
                     DLSKRAKDMGSAAVLLSLILCSGIWLAALYQRFAP"
     gene            25781..26368
                     /locus_tag="VGA59_03250"
     CDS             25781..26368
                     /locus_tag="VGA59_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX9718722.1"
                     /translation="MTRAFSLCVYCGSRTGAAAKFAAAAAAVGRWIGEHGGQLVYGGG
                     NNGLMGIMADATLGAGGRVVGIIPRVLVEKEWAKLDCTELHVVDNMHDRKRMMAERAD
                     AFLALPGGIGTFEELFEVWTWRQLGYHDKPVGMLNMDGYYEPLLDFLRTSVQYGFMSD
                     WQMELVRVGSDIETLLPALIQAAGLTSGKVNLEEI"
     gene            complement(26410..26748)
                     /locus_tag="VGA59_03255"
     CDS             complement(26410..26748)
                     /locus_tag="VGA59_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX9718723.1"
                     /translation="MKQITAVVKPFKLEEVREALAECGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEVVVKDDDVDRCMDAIVKAARTGKIGDGKIFITSVERVVRI
                     RTGEIDESAV"
     gene            complement(26937..28601)
                     /locus_tag="VGA59_03260"
     CDS             complement(26937..28601)
                     /locus_tag="VGA59_03260"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX9718724.1"
                     /translation="MALKLCIAQLNFVVGDTAGNAQRIIAAARTAYAQGARLVLTPEL
                     SICGYPAEDLLLRPAFIAACDDAVKTVALELAGLKGLFVVVGHPTGGDIRSKSVAVQR
                     RFNAASLLSEGRVLETYAKRELPNYQVFDEYRYFTRGKGTCVFHVDGVNVGLLICEDA
                     WFDEPAQLARESGAELLAVINASPFHVGKGGERVARMAERARAVGLPLVYAHLVGGQD
                     EVVFDGASFALNAEGTLVGRAPGFQEDLFSVQTERVRGRLELMAPIVHERSPEADLWD
                     ALVLGVRDYIGKNGFPGVLLGLSGGIDSALVLAIAVDALGRDKVRTVMMPSPYTADIS
                     WIDARDMAARLGVRYDEISIVPEFEAFKASLAAEFRGLPEDATEENIQARIRGVLLMA
                     LSNKFGAIVLTTGNKSEMATGYCTLYGDMAGGFAVIKDLLKTTVFRLARWRNIHDPYG
                     AGISPIPERIITRPPSAELRPGQVDQDSLPPYEILDAILERYMENDQGVEEIIAAGFD
                     RAVVERVARLIRVNEYKRRQAPVGIRVTHRSFGKDWRYPITGKFTA"
     gene            28600..29790
                     /locus_tag="VGA59_03265"
     CDS             28600..29790
                     /locus_tag="VGA59_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9718725.1"
                     /translation="MTAVKHNSNDYVTRVLSSPLELDPAQWNALLARQRAPSPFMRHE
                     YLAAMECSKSAEPATGWTPRFITLWREEQLEAACALYLKDHSYGEYVFDWAWASAYEE
                     HGLAYYPKAVIAVPFTPVPGARLLARDAAARQALIRAVLGWCGREKLSSLHLLFADDE
                     DVAACAAAGLMLRHTVQFHWMNNSWRDFEAFLASLAHDKRKKIRQERRKVADAGVVFR
                     WSEGRDIDKADWDFFYHCYERTYREHGNAPYLTRDFFRRMALDMPENWLLFMAERGQR
                     PIAASLIALGKQPGPQPPHDGKAAYGRYWGALERVDCLHFEACYYQPLQWCIEHGYDR
                     FEGGAQGEHKMARALMPVKTTSVHWLAHPSFAYAVDRFLQREGQGIGSYLEHLERRNP
                     FRQR"
     gene            29874..31439
                     /locus_tag="VGA59_03270"
     CDS             29874..31439
                     /locus_tag="VGA59_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9718726.1"
                     /translation="MKSLSGLDATFLYLETPETPMHVGSLNLYELPKGFKGSFHKAVR
                     QHIAKRAHLAPIFSRRLAFMPFDLGHPVWVETDRLDLDFHIRKVPGKRLTVRQVEAAA
                     ARLHGQLIDREHPLWEFYVFDDIEAPAAMGIQGKLVGFYSKIHHAALDGKGGTVLANA
                     ILDLSATPREVAPADPSRKGPRAGDLKIGEMIGAVFSNSLAQYAKLARSLPAAATSIG
                     GAVARQSVGGGEKGITSLRPKSPIKLAPRTIFNVGITRQRVFATASLPFAESKAMAKA
                     AGGTFNDIVLWICSTALRSYLARHATLPKKPLVAAMPVSLREESNKELNNQASITVVD
                     LGTHLADPIKRMNAIIASTAKVKEALAKLKSVLPTDYPSLLSPWLMGGAGKALFRTYG
                     KGGIAGRLPAVANLAISNVPGPQVPLYLAGARMVTFHPLSIVLHGLALNITVQTYAGR
                     VDFGIIADKQAVPHVQDLANALETAFEEAKGLFLPGATPMPANPSTTARKPRRKTATA
                     SSKRQSRLSSAHA"
     gene            31432..32217
                     /locus_tag="VGA59_03275"
     CDS             31432..32217
                     /locus_tag="VGA59_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9718727.1"
                     /translation="MRSSTPNHDLPAPSLALLALEFRAPLEFGCVLPAWPALQRAPAG
                     DGHTVLVFPGLSASDATTFPLRGYLNSLGYQTRGWSQGFNFGPRAGVLEAAGRGLEQA
                     CDTSGRKVSLIGWSLGGVYARELAKAMPDHVRCVITLGSPFAGPPKSTNAWRIYELTS
                     GRDIEREHGRFDLPTAPPHPTTSIYSRSDGIVAWRGSIQQPHHAHTENVEVVASHIGL
                     GLNPSTWWAVADRLAQPEGQWRPFERSGLFGLKGLIYPDPNRD"
     gene            complement(32432..32971)
                     /gene="ppa"
                     /locus_tag="VGA59_03280"
     CDS             complement(32432..32971)
                     /gene="ppa"
                     /locus_tag="VGA59_03280"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX9718728.1"
                     /translation="MSLDQVTPGKHVPDAFNVIIEIPMNADPVKYEVDKETGAIFVDR
                     FMSTSMHYPTNYGYVPKTLSGDGDPVDVLVITPVPLIPGVVVTCRPLGILMMEDEAGQ
                     DGKVLAVPTDKILAIYSGWQQPEDLNPLRLKTIAHFFEHYKDLEPGKWVKILGWEGPD
                     AARKEIMDGIASYQREHGA"
     gene            33214..35379
                     /locus_tag="VGA59_03285"
     CDS             33214..35379
                     /locus_tag="VGA59_03285"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX9718729.1"
                     /translation="MTGNSALILALVCGLAAVVYGFWARGWILSQDPGNPRMQEIAAA
                     IQAGAGAYLARQYKTIGMAGVVLAVLIGVFLDTTTAIGFIIGALLSGACGFIGMNVSV
                     RANVRTAQAATRGIGPALDVAFRGGAITGMLVVGLGLLGVAGFYWFLVGNGVLTPDRK
                     LSSLLNPLIGFAFGASLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVI
                     ADNVGDNVGDCAGMAADLFETYAVTLIATMVLGSLLVAGYPTNAVLYPLALGGVSIIA
                     SIIGTFFVKASPGMKNVMPALYKGLAIAGVLSLIAFYFVTVWLMPDNAITATGTQMRL
                     FGACAVGLVLTGMLVWITEFYTGTQYSPVRHIAEASTTGHGTNIIAGLGVSMRSTAWP
                     VLFVCMAILVSFWLAGLYGIAVAATSMLSMAGIVVALDAYGPITDNAGGIAEMADLPS
                     SVRDITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHKLESYGSSISFDLSDPM
                     VIVGLFIGGLIPYLFGAMAMEAVGRAAGAVVVEVRRQFRDIKGIMEGTAKPEYGVAVD
                     MLTKAAIKEMVIPSLLPVVVPIVVGLLLGPKALGGLLMGTIVTGLFVAISMCTGGGAW
                     DNAKKYIEDGNHGGKGSEAHKAAVTGDTVGDPYKDTAGPAINPLIKIINIVALLIVPL
                     VVQFHGVQAPATKAEAPAAVIAAGTTPVHAGAVTAAPAK"
     gene            complement(35469..36068)
                     /locus_tag="VGA59_03290"
     CDS             complement(35469..36068)
                     /locus_tag="VGA59_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9718730.1"
                     /translation="MVETETRTEVREHLHRLLDGMARAVASKGYADTTIADIVREASV
                     SRRTFYQHFSTKAECLIALYEAASRNALKVLRDSIDPQQEWQTQVERALTAYLGCMAQ
                     NPVLMRTLFIEILGLGTEGLAARRRVNRQIADFMLSVVNREQGHRRTTPLSPGMAMAV
                     VGGINELILQYIEQDRVARLPELVEPSSQLLRAVIETGA"
     gene            36173..36997
                     /locus_tag="VGA59_03295"
     CDS             36173..36997
                     /locus_tag="VGA59_03295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX9718731.1"
                     /translation="MTNLVVRRLLIDMEAPIARHWCGGDAFRTALFNALSMSFPVGEQ
                     FFIDAVRNGFKGLPADKQERYRAEVQGFVGQEATHRRLHGLYNEHLERQGLVNDWAPR
                     AARRLKQLEGLDPRHALAITAANEHFTAIMASWMLHNPDLLGGEDPRLATLWLWHSCE
                     ESEHKSTAFDLYQALDGNHEWRIAWFRRVTRFFLADTLRQTVNNLRRDGTLWKWSTWK
                     SAASYLFGKRGLIRMTYRPWRDYLRRDFHPSQQDAGASQRWLAEHRGEYVAVGGSA"
     gene            37039..38472
                     /locus_tag="VGA59_03300"
     CDS             37039..38472
                     /locus_tag="VGA59_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9718732.1"
                     /translation="MQLHYIANTAQASFSGRTIPVIDPSDGQAFDELQRGNAEDITAA
                     VRAARRCYEAVWSKLSAAERGRLLMRLSNKIAEHAEELATLEQRDCGKPTKQAHADAV
                     ALVRYFEFYAGACDKLHGDTLPYLDGYSVFTWREPHGVTGHIIPWNYPMQIFGRSVGG
                     ALAAGNVCVVKPSEDACLSLIRVAQLAAEVGFPAGAINIVTGYGHEVGDALARHEGID
                     HISFTGSPKVGTVIQQVAAERHCPVTLELGGKSPQVIFADADLDAALPMVINAIVQNA
                     GQTCSAGSRLLVEQAIYEPFLERLGKAFENLRVGPATMDLDVGPLIRASQQQRVWDFL
                     SDAHVAGIPTVAQGQVVEEAPETGYYQAPILLRDVPVDHRVAQEEVFGPVLAAMSFKD
                     EDQAVQMANATRFGLVAGVWTRDGARQLRMAKRVRSGQVFINNYGAGGGVELPFGGVK
                     SSGYGREKGFEALYGFTTLKTVAIRHG"
     gene            complement(38545..39138)
                     /locus_tag="VGA59_03305"
     CDS             complement(38545..39138)
                     /locus_tag="VGA59_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718733.1"
                     /translation="MTKTILALALAFLMAACTSIAKVEGDQVVNGKLVVNVSDAWNKV
                     TDPWEAGPYDMWTQEGIPLDHLRLWAGIRSGKALVAKPTVLFRAPGEKDARYPTFTAG
                     LPPDKLVSLFEALYANEGVVNVTRVEPTVFAGAKGVRFEFTLARRADDVTLKGVGWVA
                     VHKDELYAAMFAAPRLAFFEKLLPMAEAVVKTARIRG"
     gene            complement(39135..40328)
                     /locus_tag="VGA59_03310"
     CDS             complement(39135..40328)
                     /locus_tag="VGA59_03310"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX9718734.1"
                     /translation="MNAPDARRGFLKLACRHCLGFGALLSGLPAVAQDAAPSGSFKVP
                     GRFSRPAVDTEEGGLWNLMDREEAKLRRSPLAIRDPALGKYLSDVTCRLTDGHCPDIR
                     VHVMRTPMFNASMAPNGMMQVWSGLLLRMENEAQLAAIVGHELGHYLERHQLEQLRDA
                     KDKAMLAQIVGLIGGIAGAVGKIGILATQFAFSRQHETRADRLGMRLMKGAGYEGRQA
                     PLVWDNLLGELKITGGENVGERSAMFATHPPAGNRRDDLLELAGDAGGATAAAEYQKA
                     IAPLRYGWLQDEVKRGQYEESLVLFDRLLTGKPSDAEVLYARGEVYRLRGDKDDLPRS
                     LDDLARATAVETPPVEAFRSLGLVHKQRSDAPAAVQAFEKYLALSPDAPDAGLLKAYL
                     SELKP"
     gene            complement(40368..41072)
                     /locus_tag="VGA59_03315"
     CDS             complement(40368..41072)
                     /locus_tag="VGA59_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718735.1"
                     /translation="MPKLFRSSIVLAVVLFAGALQAQESDPKHLAPGFHARPAGSKLV
                     IVPADMELFSISAGGVLEPKADWTEAAQRNFKAALAVRRDQLGANAVELQDAELDEFS
                     ELNALQRAVAEAVFVHHTGMGMKLPTKNERLDWSLGEAVKPLKARTGADYALFTWIRD
                     SYASDERKATMIAMALLGAISFGGEQIGYASLVDLNSGRVVWFNDLRRMSGDMRDEKA
                     AMETVETLLEGFPVLQ"
     gene            41335..42096
                     /locus_tag="VGA59_03320"
     CDS             41335..42096
                     /locus_tag="VGA59_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9718736.1"
                     /translation="MRVQDKSIIVTGSGGGIGEGIARRLAAEGANVIVNDINAAMGEK
                     VVAQIKAAGGIASFFAADVTKSADVKALVQAAVTRHGKLDVMVNNAGWTHRNRPALEV
                     SEEEFDKCFAVNMKSIYLSTIHAVPALRANKGGSFINIASTAGVRPRPGLTWYNGSKG
                     AVITTSKSLAAELGPDNIRVNCINPVFNPDTGLSAQFAGGPVDEARRAKFLATIPLGR
                     FSTALDVANAALYLASDEAAFISGVCIEVDGARCV"
     gene            42150..43286
                     /gene="moaA"
                     /locus_tag="VGA59_03325"
     CDS             42150..43286
                     /gene="moaA"
                     /locus_tag="VGA59_03325"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX9718737.1"
                     /translation="MSQRFIPLIDQRFAAVAARVPAQSIPATGLLADSLGRPLRDLRI
                     SVTDRCNFRCSYCMPKEVFDKNHPYLPHSALLTFEEITRIARQFAAHGVRKIRLTGGE
                     PLLRKDIESLIEQLAALRTVDGEPLDLTLTTNGSLLARKAKALKAAGLQRVTVSLDGL
                     DDAVFRRMNDVDFPVAEVLQGIAAAHEAGLGPIKINMVVKRGTNEHEILPMARHFKGT
                     GIVLRFIEYMDVGATNGWRMDEVLPSAEVVKMIQAQLPLVQLEPSAPGETAERWRYAD
                     GGGEIGVISSVTQAFCGDCNRARLSTEGQLYLCLFATQGHDLRSLVRGTGANAAGSAS
                     DEQLASAIARIWQGRADRYSQLRGTMAPDTDTGGRRVEMSYIGG"
     gene            43427..44041
                     /gene="mobA"
                     /locus_tag="VGA59_03330"
     CDS             43427..44041
                     /gene="mobA"
                     /locus_tag="VGA59_03330"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEX9718738.1"
                     /translation="MIDPSLITGVILAGGRGSRMGGVDKGLQNFNGMPMALHTLMRLA
                     PQVGEVMINANRNLGAYESFGVPVWPDVLADYAGPLAGFLTAIERCETPWLVTVPCDT
                     PLFPQDLVARLARAAEEQDTEIAMAAAREEDGQIRAQPVFCLLRVNLLESLVRFTHGG
                     GRKIDAWTSQHKTVVVPFDQPGDEPRAFFNANTLAELHLLEQAK"
     gene            44038..45306
                     /gene="glp"
                     /locus_tag="VGA59_03335"
     CDS             44038..45306
                     /gene="glp"
                     /locus_tag="VGA59_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX9718739.1"
                     /translation="MKTLQEIAAQLQGYDPQALSAQAVNDFLARLVEPVGGIEHVDVF
                     DALDRVLAQDIVSPISVPPHDNSAMDGYAFDGAQLKATELLTLEVAGTAFAGKAWQGS
                     VAPGQCVKIMTGAIMPAGTDTVVPQEFIEDKGDGRISVPPKLLQPGDNRRLRGEDLHE
                     GQPALARGARLTPAACGLIASLGLKAVAVYRRLRVAYFSTGDEILSLGEVPREGAVYD
                     SNRYTVFGLLTRLGCEVIDMGVVRDDPALLEAAFREAAAKADAIVTSGGVSVGEADYT
                     KAMMKKLGDVAFWKIAMRPGRPMAVGRIGPAILFGLPGNPVAVMVTFLAFVRPALLRM
                     MGCSSAQPPQLKARSEEPIRKKAGRTEYQRGVVTTAANGSLQVRTTGNQGSGVLSSMV
                     QANGLIVLHHAQGNVAAGDPVDVMMFEGVI"
     gene            complement(45303..46415)
                     /locus_tag="VGA59_03340"
     CDS             complement(45303..46415)
                     /locus_tag="VGA59_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX9718740.1"
                     /translation="MAATLWIGTRKGLFIAHRGDSGWRLGKPHFAGEPVTQVLVDPYD
                     GAWYAALRLGHFGIKLRKSLDRGASWQEMAAPAFPPKPTEGPWKDDATPWTVDQVWAL
                     EASPDGRLWAGCLPAGLFSSRDGGASWQLVESLWDRPERKDWIGGGYDYAGIHSILVD
                     PRDPSHVTVAISCGGVWQTRDGGASWTNTSAGMVADFMPPERREDPNIQDAHRIDQCL
                     AQPDVLWCQHHGGLYRSTNAGLKWEAIAAPQPSGFGFPVAAHPQDPQCAWFVPAHSDA
                     QRMPADGRLVVNETRDGGASFVSHGEGLPQHDAYHLIYRHALVAGKEGRTLAMGSTTG
                     GLWVSEDAGVSWRCLSRDLPPIAVLRIGGDGVQSRP"
     gene            complement(46418..>46693)
                     /locus_tag="VGA59_03345"
     CDS             complement(46418..>46693)
                     /locus_tag="VGA59_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX9718741.1"
                     /translation="TNMPTVEFAPALTRHVACRPQHVQAATLRDALNAAFLAAPALRG
                     YVLDEQGAVRKHVAVFVNARMIESRSDLDVAIGPGDKVMVIQALTGG"
BASE COUNT         7859 a        15508 c        15026 g         8300 t
ORIGIN      
        1 gaccccgcca ccggcctgtc gctcaagcag ttcgacccgg ccattgacgc gttggccgat
       61 gacgtcgcgc agtgaacgct gcgcattgcc gagcacgacc gcaggggatt ggtgcgtcca
      121 gacacgaaaa cacggcgctc catcgagcgc cgcgagctgt gcgatgttcc acgcttgttc
      181 ttcagcggcg gtgcttacgt tgactccttc agcagggcga tcggcttgcc tcgggcgaag
      241 gtttcctcgc tcttgaccag gatcttctcg atgcggccgt cggccggagc gacaatttcc
      301 tggttggtct tgacgatgac gacgttggcg atgggctgac cggcgcggac cgtatcgccc
      361 tcgccgacca gccatttgtc caccagtgct tccgttcccg gatcgatgtc tttccacgca
      421 tcggcggcca gggtgatctc gatcatgcag caagtctttc ggcaacacga gctttcatca
      481 ccttctgtac ggcatcgaca atgctgccca cttgcgggat gacaaactgc tcaagcggcc
      541 gactgtaggg aatcggcacg ttgggcaccg cgagccgttt gatcggagct ttcagcgtca
      601 atgtgtccag gttctccgcg acgaccgccg agatttcgcc gctcaatcca aagctcaggt
      661 aatcctcgtc ggcgatcagc aggcgcccgg tctttgcgac cgactcgagc accgcggcct
      721 tgtcgagcgg caccagggtg cgcagatcga ggacttccgc gctgaccccg tcgcgcgcca
      781 gttcagcggc cgcgatcagc gccttttgca ccatctggct gatggtgacg atggtgacat
      841 ccttcccctc acgcgcaacc ttggccttgc cgaactcgat ggtgtacgcc gcttccggca
      901 cctcgttgct gctgccctcg aaatacgcca tccagggcag gcccatgatg cccttgtggt
      961 acatgaacat gaccggattg tcatcgcgga tggcctggat catcaggccc ttcgcgtcat
     1021 aggcgttgga cggcaccacg accttgatac ccggcatgtg ggcgaaggtc gcgtacaggc
     1081 attgcgagtg ctgggccgcg tcgttgtagc cgccgccgat cgccgtggtc agcaccaccg
     1141 gcagccggat gttgccgcca gccatgtagg tgtttttcgc aaggtggttg tagatctggt
     1201 ccatgcagac gccgaaaaag tcgacgaaca tcaattcgac gatcggccgc aggccggcgg
     1261 cggcggcgcc ggtcgccgtg cccatgaaag cggtttccga gatcggggta tccatgatgc
     1321 gatccttgcc gaacttgtcg agcaggccgc cggttgcgcc gaagatgcca ccatacttgc
     1381 cgatgtcctc gcccatcacg aatacattgg ggtcgcgttc catttcctgg gcgattccct
     1441 cggagatcgc ctgtgccatc gtgagtttgc gcgttgcagt catgttctgc tcctcgattc
     1501 ggtcggttgg ggttcagacg aacacatcat cgagcgcagc ctgcgcttgc gggtaggcgc
     1561 tctcgcgggc gaaggcgaag gcgtcggcga cgcgcttctt ggcgcgcgcc aggatggcgc
     1621 cttgttcgcc ttccgtcatc attcccaggc ttttcagaac gcccgccagt ttcggaatcg
     1681 gatcgttctt gcgcaggtcg gcgacttcgt ccttgggacg gtaggtttcc gggtcgccct
     1741 gaaaatggcc cagatagcga tccgtctgca cttcgatcag gcccgggcct gcaccgccgc
     1801 gcgcgcgctc gatcagaggg gcgaccgcct cgtacacttc cagtgcgtcg ttgttcggca
     1861 ccagaacgcc gggaatgccg taggccgagg cgcggtcggc gacccattcc acgcaagtgg
     1921 attccgactt cggcaccgag atcgcccatt tgttgtcttc gcaaacgaag atcaccggca
     1981 actgccaaag cgcggcaagg ttcagcgatt cgtggaacga gccctgattg gctgcacctt
     2041 cgccgaagaa ggccacggcg acccagtcct tgcccatctt tttggccgcc agtgcggcgc
     2101 cgcaagcggg aggcaggctg gcgccgatga tcccgctgca gctgaaccgg tgctccggat
     2161 cgaacaggtg catgtggccg cccttgccct tgccaagacc cgtgaccttg ccgaagatct
     2221 cggccgtcat cttgtccaga ggcacgcctt tggcgatggc aaagtgatgc ggccgatggg
     2281 tgccgaccac ggtatcttcc ttcttgagat gggcgcaaac gcctaccgcg acaggctcct
     2341 ggccggcagc aaggtgcatc tcgccgggaa ccggtccgga cccgatgtcg aacgccagtc
     2401 ctttctggat atgaggaggc agcttgcctt cgaggtagac attggccatc gtctcttcgt
     2461 agtcacgaat ctccagcatg gtttcataca tccaaagcaa cttttccttg tttgcctgca
     2521 tgtcgatctc cttaaggtga tggtttacct gaaaccgggg agccaggact tgcgccccgc
     2581 gaatgctgtt gtcaagtcga gtgctctccg ggccacgtcc gccaaccgcg gcgcatgccc
     2641 cggcacggca ctgcgatcac cggccgcggg cacgtcgggg gtggtggcgt cagactccga
     2701 catggtgctt gagcagccgg ttcacgtcgt tggcgcgttc catctgcacc atgtggccgg
     2761 cgccatcgag cacctccacg gtggcgcctg gcggcgcgct gccggcatgg tgcgcgggga
     2821 tgatgcggtc ttcgcggccc cagatcacca gcaccggctt gccggtctcc tccagccgca
     2881 accccggctg ctcggcctgc cggtcgccgc cgaacatgtc gcggctgagc gcgccgagca
     2941 gctcaggcac accatcgaga cgcttgtact tcagcacgtc gtcgaccatc tggcgacgca
     3001 ccagctccgg cttggcaaag agctgctcca gcacccgctt cagctcgcgc cgcgacggcg
     3061 ccgccacgaa gtcttcggtg aagccggcat tgatctccgg cccgaggccg gcggctgcga
     3121 tcagcgcgac cgacgcgaca cgccgcggat gatccagcgc catctgcgcg gcgatcgccg
     3181 cgcccatcga gtggccaacg agatgcgcct gctcgacccc gagcacgtcc atgaagcggg
     3241 cgacgaagcc ggccagcgcc ccgatgccgg tgcccgggag ccgaaccgtc gattgcccgt
     3301 ggccgggcag gtcgagcgcg atcaccggtg acttctcgcc cacggcatcg aggttgaaca
     3361 gccagttgtc gaggtcgccg ccgaagccgt ggatgaacag caccgggacg ccactgccgg
     3421 cccccttgcg cgcgtagcgg atgcgcaggc cgtcgacgtc ggcgaactgg taagccggcg
     3481 cggcctccgc atcctcgccc tcgctctccg ctggcgtgat gtaggccgcc acataggcat
     3541 cgatgtcggc gtcgctgacc ccgggcccgg ccatcacgcc gagcagcgcc ttcaccggca
     3601 gcgtctcgcc gtcctgcgcc accttgcggc gcaaggtacc cgggtcgggc gcctccaccg
     3661 cgttggcgat cttgtccgtc tcgacttcga ggatcggggc gccaacgctg atttcggcgc
     3721 cttcctcgac cagccaggac atcacggtcc cctccttcat ggacaagccc cacttgggca
     3781 tgacgatggg cgtaatgcca gccctcgctg aatcgtccat cagtgctttc ctcccttgag
     3841 cgtcttgcgc actgcatcgg ccacctgcgc accgcttggg atgtagaggt cttccagcgt
     3901 gggcgaaaac ggcacgggcg catgcggcgg cgtgaccatt tcgatagccg ccttcagcgc
     3961 gccgaaggcc tgcatggcga cctgggcgga gatgtcggcg gcgatgctgc agcgcgggtt
     4021 ggcttcatcg acgcatacca ggcgtccggt cttcgtgacg ctttcgagca ccgtgtccag
     4081 gtccagcggg gagagcgtgc gcagatcgac gatctcgacc tcgacgcctt ccttggccag
     4141 cgtggcggcg gcttccagcg cgcgatgcac catgaggcca taggtgacga tcgagcagtg
     4201 ccggccctcg cgcaccacgt tcgcctcgcc gaagggaatg gtgtacgagg cctcgggcac
     4261 ctcgccctcg aagccgtaca ggttcttgtg ctcgcagaag atcaccgggt cgttgtcgcg
     4321 aatcgactgg atcagcagcc ccttggtgtc gtagggcgtg ctcggacaca ccaccttcag
     4381 ccccgggatg tgggtgaaca ccggcgtgag catctggctg tgctgcgcgg cggcgcggaa
     4441 gcccgcgccg accatcgcac ggatcaccac cggggtttcg gccttgccgc cgaacatgta
     4501 tctgaacttg gcggcctggt tatagatctg gtcgaagcac acgcccatga agtcgatgaa
     4561 catgagttcc gccaccgggc gcatgccgca ggccgcggca ccgatcgccg cgccgatata
     4621 ggcgctctcc gacagcggcg tgtccatcag ccggtcgcca tgcttggcat acaggccctt
     4681 ggtgaccccg agcacgccac cccaggagtc catctcgccg tcgccgccgg cgccgccgac
     4741 aatgtcttca cccatgcaga tcacgttcgg gtcgcgcgcc atctcctggt cgagcgcctc
     4801 gttgatcgcc tgcttcatgc tgatctttct ggccatgaga gtctccttcg attagtaggt
     4861 cacgtagacg tcggtcgtga gatcggccgc ggtgggcagc ggcgcggcct tggcctgctt
     4921 gaccgcgtcc tcgatgacgg cgagtacctc gtcgccgatg gccttcagct cggcggccga
     4981 gaccacgcca gccccggtga cggccgcggc gaatttcttg atgcagtcct tgttggcgcg
     5041 gatgtcttcc agctcgcccg ggccgcggta ggtctgcgca tcgccctcga agtggccgta
     5101 gaagcgcacc atcttgcact ccagcagcgc ggggccgccg cctgcgcgcg cgcgcttgat
     5161 gatctcgccg gccgcctcgt gcacggcgaa gaaatcggtg ccgtccacgg taatgcccgg
     5221 caggccgaag ccggcggcgc gatcgacgta ggagtcgacc gcgacggcgt agtcgcgcga
     5281 tgtcgtttcg gcgtagccgt tgttctcgac gacgaagatc gccggcaggt tccacatggc
     5341 ggcgaggttc aggctttcca ggaaggtgcc ctggttgctg gcgccgtcgc cgacgaagct
     5401 gatcgccact tcgcccttgc cgcggaactt ggcggccagt gccgcgccac acgccagcgg
     5461 cgcgccggcg ccgacaatgc cgttggcgcc catcatgccc ttgctgaggt cggcgatgtg
     5521 catcgacccg cccttgccgt tgcacgcgcc gcccttgcgg ccgtagatct ccttcatcat
     5581 cgcgaccaca tccacgccct tgctgatgca gtggccgtgc ccccgatggg tgctggcgat
     5641 gcggtcgcca tcgcccagat ggatcatgat gccggcggca gatgcttcct cgccggcgta
     5701 aaggtgcaca aagccgggga tgtcgccgcg gccgaagtcg atgtgcagcc gctcctcgaa
     5761 ctcgcggatc gtgcgcatcg tgcgatagca tttcagcaac tcgtcgcggg tcaagggaaa
     5821 ggggttcttg cctgccatgg tgggtatctc ctctaaaggg tggatgacga aaggcggaac
     5881 aggccctcgc gtgcggcggt gtgcatgcaa cgggctacgt ccaccgtggt aaaggcgttc
     5941 tcgcgcagcg tgacgcgcac gtgatcgccg gcgctgaagg ccatctcgcg ctcgccgtcg
     6001 agcgcaacca cgccggcctg cagcgccacg ggaaagggct gatccgccgg catgggctcc
     6061 cagtgcgcga tgcccacctc gcgcagcatg cccggcgcaa tcggtgcgcg cagcgccagc
     6121 ctgcgggtcg ccgggtcgcc gccgagttgc agcgcgaggc cgccggcctc gcgccggccc
     6181 agcgggtgca gcagcccgcc gatggccgac agcccgatcg cctgcgggtc ggcgaaagcc
     6241 aggtaaatgg cggccaggct ctcggtcttc cacagcgcgc gcgcgccaat gtagcggtcg
     6301 gtcgagatga cggcatcgac gagcgcgatg tcgtggcgct ggccttcgtt gatcgagacc
     6361 tccagcaatt tgttcggcgc cagcgcctgc tcggcgctca gcttgcccgt cgcgtagagg
     6421 ccgaccgcca tcgccgtgac cgtcggcgcg cgcatctcgg ggaaggcgtt gttggtgccg
     6481 gtcgagagcc cggcaatggg cgtgtccgcg cattcgcgca ctgcggcgcg gtgcgtgccg
     6541 tcacctccga gcacgatgat cgcctttacg ccggcggcgt gcatcatgcg tgcggcgagg
     6601 aaggtgtcgt ccaccgtcga ggtcggatcc atgtcgagaa actcgacctc ggggaaggcg
     6661 tggtgcagat tgccttcgcg cttcaggctg cgcatcagca tggcgcggat accgccgtta
     6721 tcgggcatca tcagtacgcg ccgcacaccc atcgcgcctg ccacggtgag cacgctcagc
     6781 acgatgttca cacgatcggc aatctgcaga ttgccggcat aggcgacgat gcggcggatg
     6841 tcacgcgccg acaccgggtt ggcaatgacg ccgatgcatg gtgtggcatt cgagtgatcg
     6901 gcctgcatgc cctgtctcgc attggatacg ggtacgagca acagcaagtt gcatgcccgc
     6961 agcaaatcgg cgcgcaccgc ggacgtgcct gtgcgtcaag ggtgcttgat gcgaccgtga
     7021 tgacgcggtg tcggcgggtt tcgcggcagc gcatcgccca aacccgtgca gcggtcacac
     7081 tgagacaggt gtcacggggt gcaggtgtga cagctttgcc gagctttcgc cctcacgctc
     7141 gcagggcagg cgccttccgc ccagtctcag ctggcgcagg tcggcgtgga accgcaagcc
     7201 tcattggggt ggcgcatcgt ccaggtgatt tggcgacaca atgccaaagc gcttcatgcg
     7261 ccggtacagc gtcatgcgcg aaacgccgag ttggcgggcc accgcagtga cattccagct
     7321 cgccgcgcgc agatactgca tgagcagcag cgcttcggcc cctggcttgg cgtggtgcgg
     7381 gcgcgccgcg cgcggggcca tcgcggcacc cagttcgtcc ggcaggtcgc ccagccggat
     7441 atagccgtcg ctgcacaccg cgcgcgcgta ctcgatcacg ttgcgcagct cgcgcaggtt
     7501 acccggccag cggtgtgctc gcatccaggc gcgcacttcc ggcgccagct gcggcggctc
     7561 gccggtgaag ccctggcgca gcatccggtc gatcatccag tcgaggtcac gccgttcgcg
     7621 cagcggcgcc agcgcgattc gcgcgccgtt gaggcggtag tacaggtcgt cgcggaagcg
     7681 cccctcgcgg accagtgcct cgagatccca gtgcgtcgcg gcgatcaccc ggatgttcac
     7741 cgccaccggc tgcgtcgcgc cgaccggcag cacctcgtgc tcggccagca cgcgcaacag
     7801 gcgcggctgc aaggcgcgcg gcatgtcccc gatctcgtcg aggaacaggg tgccgccgtc
     7861 ggcctcctga atgagcccgc gcttgccctt cggtgccgcg ccggagaagc tccccgacag
     7921 gtggccgaac agctcgcttt cgatcagcgt ctccggaatt gccgcgcagt tcaccgcaac
     7981 aaagggccgg ctgcgccgct cgctgctctc gtgcagggcc ttggcgaagt attccttgcc
     8041 ggtgcccgtc tcgcccgtca gcaacatgct cacgggcgaa ttgatcagcc gcgccgcgcg
     8101 ctcgatctgc cgctcgagca gcgggtcgcc gccggacagc gcggcgagcg ccgcgggcac
     8161 cttgcgttcg ctgggctggc ccggcggcgg cgtccagcgc gccgacgggg gcgatgccag
     8221 catgaacagc agccgcgcac tgccggccag cgtgatggca cgctggtccg aggggcgaac
     8281 ctgcacgaaa cggccgaggt cgtcgaacga cgcgttgaag agctgcgcga acggccgtcc
     8341 gagtatcggc gcgtgcccgt cggcttcgag cagcaattgc gcgcgccggt tgtggccgac
     8401 gacgcggccc gtcgcgtcca ccgcgagcag gtactccggg ttcatgtcca ggaactgcgg
     8461 cgcggcactc agccgcagga cccaatcgtg cctgaactgg tgcaggaagc tcgcgttctc
     8521 gacatggtgg gcgtatacct tgaccagctg caaggcgaga tgctggctcg ccttgggcag
     8581 gggcgaggtg agggccgaga tgtcgagcac cgccttgagt tgcccctgcg cgccatacac
     8641 cggcgcggcg gtgcaggtga gcgctatgtg cgtcgcgtcg aaatggtcgc cctggtgcac
     8701 ggtcagcgcg tgaccggtgc tgatgcaggt gcctacgccg caggtgccgg cactgacttc
     8761 gttccagtcg gcgccgaggt acaggccggc gcggcgcagg ctgtggtcga actgcagatc
     8821 gccgatgaag tcgaccgtca cgccgcgcgc atcggtgagc agcaccgcgt atcccatgcc
     8881 cgcgaccttc cggtacagcg tctccagccc gtggcgcgcg atctggagga actcctccat
     8941 ctgatcctgg tgctcgcgca ggcgttcgcc cggcaggatg acggcttccc gcggccgcgt
     9001 ggggtcgagc cggtgctcgt gcacgcagcg ctgccaggag gcgcggatga tctcgtcgtg
     9061 cgccagcgtc tccggcgcca gtatgtcgtc accggcgacc tgcatcaccg ttgcgatgtg
     9121 ctcgcgctgc tgcagctgca tgatgggcct cctcgtcagg ggagcgcacc gcggcgccgg
     9181 gtttgcttcg cccgctcgcc gcactgcctc cggccaatcg ggactctact cctgctcgcg
     9241 cgctgtggca cgtcggccgc tgtgtacccg ggttagctgg acaattgacg atgccgcgca
     9301 ctgctgcgct ggccgagcct gtggaggcgc gcgcctcgtt caaggcgcgt cggacgtaga
     9361 ctgaagtggc gcgaacgagc gccacgagac ggtgtaccgc gcctgctcct tcctgccgct
     9421 gatcggtctg cttactgcgt tcttgccgaa cgtggagcgg cgccggcggc cggtctaata
     9481 tccagtaccg accccgctgg aaaccgcgcc gaatctaggc ccaattccgc gaatttgctg
     9541 aattatttta gacagcgcgc accccaaaac ggagcaacgg cgcggcccgg cggccaattg
     9601 ccaaccccgc ctgttaatcc gtaggtccct ggttcgagcc caggtcgagg agccaaaata
     9661 ggtacggaag tgggcaccct cgcggtgccc atttcttttt ttccgccgcc gccgccatcc
     9721 tcttggggtc ggacaccatg taggaaaatt tctggtcgag cttctcgaac cacgtggttg
     9781 tcattccggc accgcggctg gcgcatctca gcagtccgat caacggcgtt ggcgtcgcca
     9841 cctggccaag aacccaaaac cgcgtttcag gtgctatatt ttgagacagg tcagcggaag
     9901 gcgggagacc agtcgtagcc aatcctcgag gaggaacgat gagaaatcat gcttgcagac
     9961 tggttgctgc catcggcctc acactgcttt cgccctcggc attttctgcg ctggaggacg
    10021 cgcagtcgat atttggcaaa gccgtgatgt acgcgttctt tctgttcgtg attgttgcgg
    10081 cagccgtcgt ctatttcatg cgattccaag acaagcgccg gacgccactg cataaggtat
    10141 tcgagcaggg agaagcgatc cactcggtcc gacccgatgc cctggtcagc gagtgcgcgt
    10201 ccaggatgac ctcggagaag atcggtgccc tggtcgtcat ggatggcgaa cgactgatag
    10261 gcatcttcac ggaacgcgat gcactgagca gagtgttggc gcaaggcctc gatccgggcc
    10321 gcacgaaaat ctccgacgtg atgaccaggg atccctattg catcccgccg acaacgaccg
    10381 tcggcgaagc gatgcggttg gtcaccacgc ggcggttccg gcacctgccc attgtggaaa
    10441 acaacaaggt tctcgccgtc atatcgagcg gcgacctgac ccattggctt gtcaaggacc
    10501 agataggcga ggttcaggaa cttgtcgacc tcgccgcccg ctcctgaccc ccaccggctt
    10561 ttcggttagc atctttgctt gacgaccgtc aagagagctt ttccttattc tggttctaat
    10621 ggagcctgct tttagctttt tggctcgtcc taagaccgct catgctttgt cagccgtgcc
    10681 ttcgtacggt gaaaaggcat gcctctgact tggttagttc tttcgatttg gagttgccca
    10741 tgaacgaaat caacgagaag gtgtcgatgt gggccaagct gcacaaacgg tgggagaagg
    10801 tggagtcgga cttgcgctcc gtcaagacgc tgcccgcccg cgaggtcggc gtgaacatat
    10861 ccgccatgga agtcgagttg agggcgttga gggccaaggt cgacaccgcg ttcaatgaag
    10921 cctccggcgc gctgcgcgac aaaagcgccg caaaaacgcg ggatgaccac cgagagctgc
    10981 tgtaagcagc gttgacgcga ggcagtcgaa aacacggcga tgccttccag ccaatgactc
    11041 cgggaggcag cggccgtgct gatctcaacg agcggcaaag cgagcctcgc gagacctgac
    11101 cgcaactgaa ggcctgcaac ggcggttccg atcgcggaca ccgcacaata gcggtgtgca
    11161 atacggtccg atatgacgtt gtcacgcccg cgatgaatct tccctggctc gatcccggcg
    11221 aacccttccc cgaagcgagc gaagcgtggg acacatcgca gccggcgccc ggcctgctcg
    11281 cggccggagg tgccctcgat gtcgacagcc tgcaccgtgc gtactcggcc gggatctttc
    11341 cctggttcag cgaaggccaa cccatcctgt ggtggagcac ggatccgcgc atggtgctgt
    11401 tcacccgcga gttcaagctg cacaggtcgc tgcgcaagac gctggcacgg ttcgtcgccg
    11461 atccgcgttg ccagttgcgc atcgacacga atttcgccga agtgatccac gcctgctcgc
    11521 tgagcgaacg ccccgggcag tccggcacct ggatcgtgcc gcagatggtc aaggcctatg
    11581 aggcattcca tcgcgccggt ttcgcgcaca gcgtggaaac ctgggtcggc ggacggctga
    11641 tgggtggtct ctactgcgtt gccatcggcc gggccgtttt cggcgaatcg atgttcacgc
    11701 gcatgcccga cgcatccaag atcgccctgg ccggcctgac tgcgttctgc cggcatcatg
    11761 gcattgcgat gatcgactgc cagcagaaca cccagcatct tgcgtcgctg ggtgcgcgcg
    11821 aaattccgcg tgccgatttt gtggcgcaag tctcacaaaa cacgaagaaa gcccctgcgc
    11881 catggcgctt cgaccccgta tactggaacg aacttctgcc gccgaatccc tcagcgtgac
    11941 acacctcaag gatcttccgc tacagaccct gcagttctat gcgacggcac cgtacccgtg
    12001 cagctacctg ccgggcaggc aggcccgatc gcaggtggcc actcccagcc acctgatcca
    12061 taacgacgca tactcggacc tcgtcgtcaa cggcttccgc cgcagcggca tgttcaccta
    12121 ccggccctac tgcgacggtt gccgggcctg cgtgcccttg cgggtactca gccagcagtt
    12181 ccgtcccgac cgcagccagc gccgcgcctg ggtacgccac cagaacctgc aggcaagggt
    12241 gctcaagctg tgcttcgtgc ccgagcacta tcacctgtac ctgcgttacc agaccggccg
    12301 ccatgcgggg ggcggcatgg accacgacag catcgatcaa tacacccagt tcctgttgca
    12361 aagccgagtc aattcgcgcc tggtggagtt tcgggaaacc cgggcggacg gttcggccgg
    12421 cgccctgcgg atggtgtcca tcctcgacgt gctgaacgac ggcatctcgg ccgtctacac
    12481 gttctatgag ccggaggggg ccgcgagcta cggaacctat agtgtcctgt ggcagatcga
    12541 gcaggcgcgg cgaatgcggc tgccgcatgt ttacctgggc tactggatcg gccaaagccc
    12601 caagatgaac tacaagtccc ggttcatccc gcacgagatc ctcgtcgaag gccattggat
    12661 tcgaggtacc ggcggctgat cctgctgcgt tacttccagc cgcaggttgg gatttcacat
    12721 gataaaatgc aggctttaat tcagccgacc ttcccccatg agaaaaatcg acccgggcgc
    12781 gcacgccacg cccaccatcc aggtgatcga acgcatgttc ctgctcatcg acgtgctggc
    12841 ctcgcacgag gactccgtgt cgctcaagga gatcagcgaa aaaaccgggc tgcacccgtc
    12901 caccacccat cgcatactca acgacctggc cactggccgg ttcgtggatc ggcccgaggc
    12961 cggcagctat cggctcggca tgcgcctgct ggaactcgga aacctcgtca aaggccgcct
    13021 gaacgttcgc gatgccgcgt tgacgccgat gcgcgagctg cacaagctga tccagcaacc
    13081 ggtcaacctg agcatgcgcc aaggcgacga gatcgtctat atcgagcgcg cctacagcga
    13141 gcgctcgggc atgcaggtgg tacgcgccat cggcggccgt gcacccctgc acctgacctc
    13201 tgtcggcaag ctgttcctgt cagccgacga tccgcagcgc gttcgcgcct acgccacgcg
    13261 caccggactc gcgggtcaca ccaagaacag catcacgcag ttgcccatcc tcgagcgcga
    13321 gttgtcgaag gcgcgccagt acggcatcgc cagagacaac gaggagctgg agctgggcgt
    13381 acgttgcatg gccgcgggca tctatgacga tcagagcaaa ctgatcgcgg gcctgtcgat
    13441 ttccgcgccc gccgaccggc tggacgaagg ctggttgacg aagctgcaag caacggccaa
    13501 cgagatctcc gcggcgctcg gcttcaagac gccgccccaa cgcgccgccg cgtaagcggc
    13561 gccaaaagga cacggcccca tcggggccgt gttttgtctg gagcgaagcg gttaaatagc
    13621 ggttaaccgg ttaatttttg acctggtgaa agaccgcgtt ggcggctggc ggcaccagcg
    13681 ggttcgattc cacccagcgg cgcacccgtt cggcgtcgcc gatgcggctg agcttgccgg
    13741 ccgagtccag gaacaccatg atgagcttgc gcccggcgat cttggcctgc agcacgaggc
    13801 actggcccgc ctcggagatg tagcccgtct tctgcagccc gatgtcccag tcggggttct
    13861 tcaccagccg gttggtattg ttgaagttga gtgtccgctt gccgacggcg acctggtggc
    13921 cgggcgaagt gctgtattcg cgcagcaccg gatcctcata tgtcgtattc accagcgtcg
    13981 ccaggtcatg ggcgctggac tggttctgac tggacaggcc ggtaggctcg acgtagcggg
    14041 tgtccttcat ccccagcatc tgcgccttgg cgttcatcac cgaaacgaag gcagccatgc
    14101 cgccaggata agtgcggccc agcgcatgcg ccgcgcgatt ttcgctggac atcagcgcca
    14161 gatgcagcaa ctcgccgcgg gtcagcttgc tgccgacgcg caggcgcgag cggctgcctt
    14221 tctcggtgtc cacgtcgtcc tgcgtgatct cgatcgcctc gtccatgggg agcttggcat
    14281 cactgatgat cagaccggtc atgagtttgg tcagcgacgc gatcggcagc acggccgaat
    14341 cgttcttgct gaacagcacc tcgctggtgt cctggtcgat caccagggcc acgctggatt
    14401 tgaggtcgag cgggtcctgc accgaatgca ggccggccag cttgccgtag gaggggcgcg
    14461 cgggcacgat tgccattcgc atcacggact tgcgcttgcc ggcgaacgat cgaacgactc
    14521 gtttgccggc gcttgaatgg attttcttga cggagctcgc cttggccttg cgtggcgccg
    14581 cgtcggcgac ggccggcgcc agcccgatca cgagagcggc cacggcaacc gaatggagga
    14641 gacgattcaa tactttgctc atcacagacg gtgctttgtg gtgcatcggg actccagaaa
    14701 cgctacaagg gcacacgaag tttagtcaga aagaaaaagt tgcgcaagat caatgactta
    14761 tgtaactttc ctaactcaat acagtaatta tgcggtaggc gctatccttg tgcggccact
    14821 ctgtccgatt tattttgtaa cttattcagt gcactcagat acgcttttgc cgaagccacc
    14881 acgatgtcgg ggtccgcgcc caccccattg acgactcgcc cggagttctg cagccgcacc
    14941 gtgacctccc cttggctttc ggtcgagccg ctgatcgcat tgaccgaata gagcaccatc
    15001 tcggccccgc tcctgacgtg cgattcgatt gccttgaggg aggcgtcgac cggaccgttg
    15061 ccatccgatt cgccccgcac ctcctttccc tcgaccgtga aaacgatgac ggcgtggggc
    15121 cgttcgcccg tttcgctgtg ctgggccagc gagacgaagt ggtattgctc ctgctcgtgc
    15181 gtcacgctct catcgctgac gagcgccagg atgtcctcgt cgaagatctc gctcttgcgg
    15241 tcggcgagtt ccttgaacct ggtgaaggcc gtgttgacgt cggcctcgct gtccagcgcc
    15301 acgcccagct cctgcagccg ctgcttgaag gcattgcggc ccgatagctt gcccaggacg
    15361 atcttgttgg cgctccagcc cacgtcctcg gcgcgcatga tctcgtaggt gtcgcgggcc
    15421 ttcagcacgc cgtcctggtg gatgccgctg gcgtgggcga atgcgttcgc gccgaccacc
    15481 gccttgttgg gctgcaccac gaagccggtg gtctggctga ccatgcggct ggccgcgacg
    15541 atgtgccggg tgtcgatgcc gacgtccagg ccgaaatagt cgcgccgcgt cttcacggcc
    15601 atgacgacct cttccagcga gcagttgccg gcgcgctcgc ccagcccgtt gatcgtgcac
    15661 tccacctgtc gcgccccgcc gatcttcacg cccgccagtg aattggcgac cgccatgccc
    15721 aggtcgttgt ggcagtgaac cgaccagatg gccttgtccg aattgggaat gcgctcgcgc
    15781 agcgtcttga tgaacaagcc gtacagctcg ggcacggcgt aacccacggt atcgggcacg
    15841 ttgatcgtgg tggcgccctc ggcaatcgcg acctccagga ccctgcacag gaaatccatc
    15901 tcgctgcggt acccgtcctc gggcgagaac tcgatgtcgt cgcacagatt gcgggcaaag
    15961 cgtaccgact gcttcgcctg ctccagcacc tgctcgggcg tcatgcgcag cttcttctcc
    16021 atgtgcagcg gcgaggtcgc gatgaaggtg tggatacggg cgcgctgggc ttccttcagc
    16081 gcctcggccg cccgcgagat gtcacggtcg ttggcgcgcg acagcgagca gatggtcgat
    16141 tccctgatgc ccttggcgat ggcctggacg gcctcgaagt cgccgttgga gctcgcggca
    16201 aagccggcct cgatcacgtc caccttcagg cgctcgagct ggcgcgcgat gcgcaacttc
    16261 tcgtccttgg tcatcgacgc gccgggcgac tgctcgccgt cgcgaagggt ggtatcgaaa
    16321 ataatcagtt tgtctgccac ttgaatcgct ccttacctgg tgagtcgggg actgaagctg
    16381 gagatccgtc ccgcaagaaa acctagctgg acagagctgc ctcgctcgtg ctgcccgccc
    16441 gcgtccggcc caggcccgaa acaatggcct tgaacgaggc ggaaacaata ttggagtcca
    16501 tgcccacccc gaacagcgtt cgcgctccga tgcgcagctc gaggtaagcc acggcccggg
    16561 cattggcgcc cgcgccgatc gcgtgctcat ggtagtcgag cacgcggacg gtctcgccgc
    16621 aagccgaagc cagcgcttgg acaaaagcgt cgatcggacc gttgccttcg ccctggatat
    16681 tcaggcgccg ccccgacagt tccacctcgg ccgccagctt gaccagcttg ccgtcccttc
    16741 cctgcgcctc ttccagcacc aggtgctgcg gcgcggcgat gcttcgaatg ccgtactcgc
    16801 gctcgaacaa ttcgaacaga tcccgggccg tcagttccct gcccgactcg tccatcacgg
    16861 cctgcacgac ccggctgaac tcgatctgca gacggcgcgg caactccagg ccgtactcgc
    16921 tctcgagcag gtacgagatg ccgcccttgc ccgactggct gttgacgcgg atgacggcct
    16981 cgtagctgcg gccgaggtcc ttgggatcga gaggcaggta gggcatgtcc cagacgtcgc
    17041 cgtccgcctt gcggccggcg aaagctttct tgatggcgtc ctggtgggag ccggaaaacg
    17101 atgtgtagac cagatcgccc acgtaggggt gccgtgggtg aatcggcatc tggctgcaat
    17161 gctcgaccgt gcggcggatt tcgtcgatgt cggagaagtc caggcccgga gcgatgccct
    17221 gcgaatagag gttcaacgcc acattgacca ggtcgaggtt gccggttcgc tcgccgttgc
    17281 cgaagaggca gccttcgagg cggtccgcgc cggccatcac cgcgaactcg cccgcggcgg
    17341 ttccggtgcc ccggtcgtta tgcggatgaa ccgacagcac gatggcttcg cggcgcgcca
    17401 gattgcgatg catccattcg accatgtccg cgaaaatgtt gggggtggag tgctccaccg
    17461 tcgaaggcag gttgacgatg cacctggcct ggggcgtggg ctgccagacc tcggtcacgg
    17521 catcgaccac ccgcttggag aaagccagct ccgtaccgga aaacatctcc ggtgagtact
    17581 cgaaggtcca gcgcgtttgc ggctgctggg cggcgagctc ccggaacagt cgcgcccgac
    17641 tcgtcgccag ctcgacaatc ccgtcctcgt ccaggcccag caccaccttg cgcatgaccg
    17701 gcgcggtggc gttgtacagg tgcacgatga cgcttttcgc tccccgcagc gactcgaacg
    17761 tacgccggat caggggctcg cgcgcctggg tcagcacctg gatggtgacg tcggcgggaa
    17821 tcaggtcatc ctcgatcagc ttgcggatga aatcgaactc ggtctgcgaa gcggacggga
    17881 atcccacctc gatttccttg aagccgatgt ccaccagcat ctcgaacagg cgcagcttgc
    17941 gcgccacgtc catcggctcg atcagcgcct gattgccgtc tcgcaggtcc acgctgcacc
    18001 agatgggcgc gcgggtcagc acggcgtcgg gccaggtccg gtcggtcaaa cgcaccggcg
    18061 cgaaggcgcg gtacttgctg gcgggattct tcaacatgat cgtttctcgt gggtttcggg
    18121 tgaaccagca gcctcaaaga aaaacggccc gctgctggtg catacgggcc gttgtcaggt
    18181 gaacgcgtgc gctaccgtct ccgcccgtgg ggagatagca gtagcgccag cgaaattcgg
    18241 ttcatggatg ggaatgtagc acaagcggcg ttggggggcg cacgacggga cccaggaccc
    18301 tacttatgca gcccgcgctc gtcgggctct tcggtcgaaa tactgatcac gctggcttgc
    18361 tggcctttgg ccttgcgcca gccatacagg acataaccgc tcaagccgta gacgacaaac
    18421 aggccgaaca ggacgatcgg gggatggatg ttgatgaccg ctatgcccag ggcgatcagg
    18481 acgatcacca cgaacgggac gcttttcttc atctgcacgt ccttgaagct gtagaacggc
    18541 acattggtca ccatggtcag gccggcgtac agcgcgcagg cgaacatcgg ccaggccacc
    18601 tcggagccct tgacctccag gtcgttcatg acccagatga acccggcgac cagggccgcc
    18661 gcggccgggg acggcagccc ctggaaatag cgcttgtcga ccacgccggt gttgacgttg
    18721 aagcgcgcca gccgcaaggc cgcgcaggcg cagtacacaa aggcggcgat ccagccccag
    18781 cgacccaggc tcttcaggcc ccactcgtag gcaatcagcg ccggcgccgc tccgaaggac
    18841 accatgtcgg acaacgagtc catctgctct ccgaacgcgc tctgcgtgtt ggtcatgcgg
    18901 gccacacggc catccaggct gtcgagcacc atggcactga acacgcccac cgccgccagg
    18961 tcgaagcggc cgttcatcgc catcaccacc gcatagaagc ctccgaacag ggccgccagc
    19021 gtgaacaggt tcggcaggat gtagatgccc ttgcggcgct tgcgcaccac gacaccctca
    19081 gtgaagggag cggactggga gtcgttaccg tcgtgcataa gacccgaagt gtaaggtgtc
    19141 attgcgggcc tcgacgggaa gatcgctggc tcacgaggtg tggatcccgg gtcacgcccg
    19201 ggcatgacag ctcgcgagcc ggcgcaacgt caattgcgcg attgatccac cagcttgttc
    19261 ttcttgatcc agggcatcat ggcgcgcagc gtctcgccca cggtctcgat ctggtgttcg
    19321 gccgtcaggc ggcggcgcga cagcagcacc ggcgcgccgg cgcggttctc caggatgaag
    19381 ctcttggcgt actcgcccga ctggatgtct ttcaggcact gcttcatcgc ctgcttggtg
    19441 gcatcggtga tgatcttggg cccggtcacg tattcgccgt attcagcgtt gttggagatc
    19501 gagtaattca tgttggcgat gccgccttca tagatcatgt ccacgatcag cttgagttcg
    19561 tgcaggcact cgaagtacgc catctcgggt gcgtagccgg cttccacaag cgtctcgaaa
    19621 cccgccttga tcagctcgac agtgccgccg cacagcacgg cctgctcgcc gaacaggtcg
    19681 gtctcggtct cttcccggaa gctggtttcg atgatgccgg ccttgccgcc gccattggcc
    19741 atggcatagg agagtgccag gtcacgggca cggccgcttt tgtcctgatg caccgcgacc
    19801 agctgcggca cgcctccgcc ctgcgtatag gtatggcgca cggtgtggcc cggcgccttc
    19861 ggcgccacca tccagacgtc caggtcggcg cgcggggtga cgaagccata gtgcacgttg
    19921 aagccgtgcg cgaacaccag cgaagcaccc tgcctgatgt gcggctcgat atctttctgg
    19981 tacacggcgg cgatctgctc gtcgggcagg agaatcatga ccacgtcggc cgaacgcacg
    20041 gcttcggcaa cttcggcgac cttgaggccg gccttttcca ccttgcccca ggacgcgccg
    20101 cccttgcgca ggccgaccac gaccttgacg ccgctgtcgt tcaggttctg ggcatgcgcg
    20161 tggccttggg agccgtagcc gatgatggcg accgtcttgc ccttgatcag gctcaggtca
    20221 cagtccttgt cgtaataaac cttcatggtt cttttctcca gttaatgcgt gatgttggcg
    20281 ttggttcaaa cccgcaggac ccgctcgccc cggccgatgc cgctggagcc ggtgcgcacc
    20341 gtttccagga tggcgctgcg gtcgatggct tccaggaaag cgtcgttctt gccctggttg
    20401 cccgtcagct cgatggtgta gcttttttcg gtcacatcga tgatgcggcc gcggaagatg
    20461 tcggccatgc gcttcatttc ctcgcgctcc ttgccgacgg cccgcacctt caccatcatg
    20521 agctcgcgct cggtataggc gccctcggtg agatcgacaa ccttgacgac ttcgatcagg
    20581 cggttcaagt gcttggtgat ctgctcgatc acgtcgtccg agcccgtcgt ctggatcgtc
    20641 atgcgcgaca gcgacgggtc ttccgtcggc gccaccgtga gcgattcgat gttgtagccg
    20701 cgggccgaga acaggccgac cacgcgcgag agagcaccgg gctcgttttc cagcaatacc
    20761 gcgatgatgt gtttcatgac tgatttcctc ttttcggtgc cctccccgtc cggcggtaac
    20821 cgggcggctt ggcagccaat agattcttcg gggttgtcat cccggacttg atccgggatc
    20881 cacgttggcg cgccagcatg gattgcgggt caagcccgca atgacaggct cttgtgttta
    20941 aaggtcttcg gatcccagca gcatctccgt gatgcccttg cccgccttca ccatcgggaa
    21001 cacgttctcg gtgggatcgg tgcggaagtc catgaacacc gtgcggtcct tcagtttgcg
    21061 cgcctcgcgc agcgccggtt ccacgtcctt ggggtgctcg atcagcatgc ccacatgccc
    21121 ataggcctcg gccagcttca cgaagttggg cagcgcatcc atgtagctgt ggctgtaacg
    21181 gccctcatag tcgatctcct gccactgacg caccatgccc aggtagcggt tattgagcga
    21241 aatgatcttg atgggcgtgt tgtactgcag gcaggtggaa agctcctgga tgcacatctg
    21301 gacggaaccg tcgccggtga cgcaatagac ctcgctcgcc ggcttggcca tcttgatgcc
    21361 catcgcgtaa ggaatgccca cgcccatggt gcccaggccg cccgagttga tccatcgccg
    21421 cggctcgttg aaccggtaat actgcgccac ccacatctgg tgctggccca cgtccgaggt
    21481 gatgtagacg tcggcgtcct tggtcatgtt ccacagcgtt tcgatcaccg cctggggctt
    21541 gatgacctcc gtgttcttgc ggtcgtagcg caggcagtcg cgcttgcgcc agccctcgat
    21601 cgtgtcccac caggcgccca gggcgtcggc atccggcctg gtgcccgatt ccttgagcat
    21661 ggcgaccagt tcgcccagca cttccttgac gtcgccgacg atcgggatgt ccaccctcac
    21721 gcgcttggag atgctggacg ggtcgatgtc cacgtggatg atcttgcgct cgttctggcc
    21781 gaagtgcttc gggttgccga tgacccggtc gtcgaaacgc gcgcccacgg ccagcagcac
    21841 gtcgcagttc tgcatggcat tgttcgcctc gacggtgccg tgcatgccca gcatccccag
    21901 gaacttgcgg tcgctcgccg ggtacgagcc caggcccatc agggtgttgg tgcaagggta
    21961 gcccagcata tccacgagcg tgcgcagttc ggccgtggcg ttgcccagga cgacgccgcc
    22021 gccggtgtag atgtagggcc gcctggcggc cagtagcaac tgcagcgcct tgcggatctg
    22081 tccgccgtgg cctttgcgca ccgggttgta ggagcgcatt tccaccttgt ccgtgtagcc
    22141 ggtataggcg gtcttgttga acgagacgtc cttcgggatg tcgaccacca ccgggcccgg
    22201 ccggccgctg cgggcgatgt ggaaggcttt tctcatgact tcggccatct gccgaacgtc
    22261 cttgaccagg aaattgtgct tgacgacggg ccgcgtgatg cccacggcat cgcactcctg
    22321 gaacgcatcc atcccgatgg cgtgcgtcgg cacctggccg gtgatgatca ccatcgggat
    22381 gctgtccatg taggccgtgg cgatgcccgt aaccgcgttc gtcacgccgg ggccggaagt
    22441 gaccagcgcc acgccgacgt cgcccgtggc ccgtgcgtag ccgtccgccg cgtgaaccgc
    22501 cgcctgctcg tggcgcacca gcacgtgctg gatggtgtcc tgcttgtaga aagcgtcgta
    22561 gatgtgcagt accgcgccgc cgggatagcc ccagacgaac ttgacgttct cggcctggag
    22621 tgccttgacg aggatttcgg cgccacgcag ttccgcgctc tggccgggcg tggtggaagt
    22681 accggcggat gccgcgttgg ccgcggcggc cgacgcgatt tcggcttttg agatttccat
    22741 cgatgaacct ttgcgaattt ctctgacgaa agaccttggg tgcccctgcc acacactctc
    22801 gtgaagctgt gtcggaactt agaacccggg accatgggcg ggcctatcgc gccgccggat
    22861 cagggctcgt ttgccttgtg acttgcaggc cgcattatcg cactgcaaca cggcaacccg
    22921 gcaatagggc ggacctcccg atgcgataat ttccggctga cggcgctgag gggcgccggg
    22981 cgcgcgtcaa aacgaagcac agcaagccgt ccaggttcag ataccttggc caccgaacga
    23041 gaactatcag acttcctcaa gagcgtcgaa aaacgggcct tcaaacgctc gatctatcac
    23101 gtgcgcgacg aggaagccgc cctggatatc gtgcaggaca gcatgatgaa actggccgag
    23161 cattacggcg acaagccgcc ggctgaactg ccgatgctgt tccagcgcat cctgtccaac
    23221 tgcacgctcg actggttcag gcgccagaag acgcgcaacg ccctgttctc caacatgagc
    23281 gatttcgaat cctcgaccga cgacggggat ttcgacttgc ttgaaactta tgccttcatc
    23341 aacgactccc agcaggtcga aagcgccgag gatgcgacgc ggcggggcca gatactgcgc
    23401 gaaatcgaat cgcagatcca ggaactgccg gtccgtcaac gtgaagcctt cctgatgcgt
    23461 tattgggagg atatggacgt ggccgaaacg gcggcggcca tgggctgctc cgaaggcagc
    23521 gtcaagacgc actgctcgcg cgcggtccag gccctcagca aggcactgaa ggcaaaggga
    23581 atacaactat gaacgacaag gcactcatcg acccccagca gtatcaggac cggtttggcc
    23641 ggcaggtcgc tgcgcgtctg tcggccggga tggccgagct gccctacggc atcaccgagc
    23701 ggctgcgtgc cgcccgtgcc caggccgtgg ccaggcgcaa acccgcgagc ctgcgcagag
    23761 ccttctctat tttcgcctcc ggcggcgcgg caacactgac cttcggcgag gacggcgcga
    23821 gcctgtggaa ccgggtcgcg gcggtgttgc ccctggtcgt gctggccgcc ggcctggtcc
    23881 tgatccatgc gatccaggat gagcgccgcg ccagcgaagt cgccgaagtc gatgccgcgc
    23941 tcctgaccga cgacttgccg ccgtcggctt atgccgaccc cggtttcgcc cagttcctca
    24001 ggttcagcaa ggaatagcgc agcgccggcg atgcctctgt tcgctcccgc cttgttgccg
    24061 tcgagacctt tcgccggggg tctgaccctt gtcctgcgtc ttgcagcggt atgcctcccg
    24121 ctgtccggct tgcctgccac tgtcctggcg cagccgcaac cgcaggccgt tggcgccgcg
    24181 tcggcggccg cgtcggcgcc cctcccggcc gccggcacgg cggcaagccg cgccggctcc
    24241 cccagggcca cggcatcggc cgcgagaaca aacaacaaca agcgcgtcac caagccgctg
    24301 tggaacgaat tgacgcccgc ccaacagcaa gcgctggcgc cgctgtccgg caaatggaat
    24361 acggtcagcg aggcgcaaaa acgaaagtgg ctggcgctgt cgcagaactt tcccggtatg
    24421 tcgggggccg agcaggccaa gctgcacagc cgcatgagcg aatgggctgc cctgacaccc
    24481 cagcagcgca cccaggcgcg cctgaatttc ggcgagacga agcagttgtc ccccgacgac
    24541 aagaaggcca agtgggaggc ctaccaggcc ctgccgcccg aggaaaagcg caaactcgcg
    24601 gccagcgccc ccaaaccccc cgccacagcg gccgccgtca agccggttcc gcctgaaaag
    24661 ctggccgacg tacccaaaaa gccggccgcc aagacgcccc gcatcgcgac atcccctggc
    24721 caggtggatc acaacacgct gttgccgcag ccgtcgccca agcgcgggga cggccgggcc
    24781 aactgacagt ggcggcgccc gtggcggcag agatcaccgg aagtaccccg gccggaccac
    24841 aactgcctcc cgagaggttg atggcacccg gcttggcgcg gcgcatggcg tgctggctct
    24901 acgaggggat cctgcttttc gcggtggtgt tcctggccgg ctggctgttc agcacgctgg
    24961 ggcaggtgcg cgacgccatg gacgcacgcc ggcccctgct gcaggcgttc ctgttcgtcg
    25021 tcttcgctat ttatttcagc tggttctggg ccaggggcca gaccctggca atgaaaacct
    25081 ggaacatccg gatcgtcgac cggaccgggc gtcccgtcag ccagctgcgc gcactgttgc
    25141 gctacgcctg gggctggctc tggttcctgc cgccgcttgc gctggtggca acgctaaagc
    25201 cgggtggcgg gacgagcgcc gtcgtcgtac tgggctgggt ggccgtgtgg gccttgctga
    25261 gccgctttca tccgcagcga cagttctggc acgacgaatt cgcaggcacc cggctggtga
    25321 gttccgtgcc actgagccgt tgagagcggc cgtcactttc ctcagcgaca atgcgggcca
    25381 tgtcctcgcc tcccgacgcg ctcacacccg cgaaccagca gaaactgcgc cgcggcctga
    25441 gccgcgtctg gcatgcggcg ggctactccc ttgccggcct tcgggccggc tggggcgaga
    25501 ccgcgtttcg gcaggaggcg gtcgcgtccg tcgcgttgct tcctgtggcc ttctggctcg
    25561 gtcgcacatg ggttgaggcc gctctcctcg ccggttcggt gctgatcgtg atgatcgtcg
    25621 aactgctcaa caccggcatc gagaccgcta tcgaccgcat cggccctgaa tggcacgatt
    25681 tgtccaagcg cgccaaggac atgggcagtg ccgccgtact cctgagcctg atcctttgct
    25741 cgggcatctg gctcgcggcg ctgtaccaga gattcgcgcc atgacgcggg cattttcgct
    25801 atgcgtgtat tgcggatcgc gcaccggcgc ggcggcaaag ttcgcggcgg ccgccgccgc
    25861 ggtgggccga tggatcggcg agcatggcgg ccagctggtg tatggcgggg gcaacaatgg
    25921 cctgatgggc atcatggccg acgccacact cggggcgggc ggccgggtgg tcgggatcat
    25981 cccgcgtgtg ctggtcgaaa aggaatgggc caagctcgac tgcaccgaac tgcatgtggt
    26041 ggacaacatg cacgaccgca aacgcatgat ggccgagcgg gccgacgcat tcctcgccct
    26101 gcccggcggc atcggcacct tcgaagagct gttcgaggtc tggacctggc ggcagctggg
    26161 ctaccacgac aagcccgtgg gcatgctcaa catggatggt tattacgaac cgctgctgga
    26221 ctttctccgg acaagcgtgc agtacggctt catgagcgac tggcagatgg aactggttcg
    26281 tgtgggcagc gacatcgaga ctttgctgcc cgcgctgatc caggccgcgg gcctgacctc
    26341 cgggaaagtg aatctcgagg aaatctgaag accgtggcga gccgcaagcg cgcccagcgc
    26401 cttcaggcct taaaccgcgg actcgtcgat ctcgccggtg cggatgcgaa cgacgcgctc
    26461 gacgctggtg atgaagatct tgccgtcacc gatcttgccg gtgcgcgccg ccttgacgat
    26521 ggcatccatg cagcggtcga cgtcgtcgtc cttgaccacc acctccactt tcaccttcgg
    26581 caggaagtcg accacgtatt ccgcgccccg gtacagctcg gtatggccct tctggcgccc
    26641 gaagcccttg acttccgtca cggtgaggcc ggtaacgccg cattcggcca aagcctcgcg
    26701 cacctcctcg agcttgaacg gtttgacgac ggcagtgatc tgtttcatgg gttcctctta
    26761 atcggtcgag gacagagctg acggtctgtc ccgcgatgta tcttaagctg tcgaggacag
    26821 agctgaaggt ctgtcccgcg aagtatctta agctgtcgag gacagagctg aagatctgtc
    26881 ccgcgaagta tcctaaagtg tcgaggacag agctgaagat ctgtcccgcg aagtatctaa
    26941 gctgtgaatt taccggtaat aggatagcgc caatccttgc cgaagcttcg gtgcgtgacc
    27001 cggatgccca ccggggcctg ccggcgcttg tactcgttga cgcggatcag gcgcgccacg
    27061 cgctccacga cggcccggtc gaatccggcg gcgatgatct cctccacacc ctggtcgttc
    27121 tccatatagc gctccaggat cgcatccagg atctcatacg gcggcaagct gtcctggtcg
    27181 acctggccgg gccgcagctc ggcgctgggc gggcgcgtga tgatgcgctc ggggatcggg
    27241 ctgatgcccg cgccgtaggg gtcgtggatg ttgcgccagc gcgccagcct gaagacggtg
    27301 gtcttgagca ggtccttgat caccgcgaag ccaccggcca tgtcgccgta gagggtgcag
    27361 tagccggttg ccatttcgct cttgttgccg gtggtcagca cgattgcgcc gaatttgttg
    27421 gacagcgcca tcagcagcac gccgcggatg cgggcctgga tgttctcctc ggtcgcgtcc
    27481 tcgggcaacc ccctgaactc ggccgcgagc gaggccttga aggcttcgaa ctccggcacg
    27541 atggagatct cgtcgtagcg caccccgaga cgcgccgcca tgtcgcgcgc atcgatccag
    27601 gaaatgtcgg cggtgtaggg cgagggcatc atcaccgtgc gtaccttgtc gcgcccgagc
    27661 gcatccacgg cgatcgccag caccagggcc gagtcgatgc cgcccgacag gcccagcagc
    27721 acaccgggga aaccgttctt gccgatgtag tcgcgcacgc ccagcaccag cgcatcccac
    27781 aagtcggctt cggggctgcg ctcatgcacg atgggcgcca tcagttccag gcggccccgc
    27841 acccgctcgg tctgcacgct gaagaggtcc tcctggaatc ccggcgcgcg tccaaccagc
    27901 gtaccttccg cgttcagcgc gaaagaggcg ccgtcgaaca caacctcgtc ctgccctccg
    27961 accagatgcg catagacgag cggcaggccg acggcgcgcg ctcgctcggc cattcgcgcc
    28021 acccgttcgc cgcccttgcc cacatggaaa ggcgaggcgt tgatcacggc cagcagctcg
    28081 gccccggatt ccctggccag ctgggccggc tcgtcgaacc aggcgtcctc gcagatcagc
    28141 agccccacgt tgacgccgtc gacgtggaac acgcaggttc cctttccgcg cgtgaaatag
    28201 cggtactcgt cgaacacctg gtaattcggc agttcgcgct tggcataggt ttccagcacg
    28261 cgcccctcgc tcagcaggct ggccgcgttg aagcgccgct gcaccgccac cgacttgctg
    28321 cggatgtcgc cgccggtggg gtgacccacc acgacgaaca ggcctttcaa tccggccagt
    28381 tcgagggcca ccgttttcac ggcatcatcg caggcggcga tgaaagccgg ccgcaggagc
    28441 aggtcctcgg cggggtagcc gcagatcgac aattcgggcg tgagcaccag acgcgcgccc
    28501 tgggcatagg cggtccgggc cgcggcgatg atcctttgcg cgttgccggc ggtgtcgccc
    28561 accacgaagt tgagctgggc aatacagagt ttgagagcca tgacagcagt gaaacacaac
    28621 tcgaacgatt atgtcacccg ggtactgagt tcgccgctcg agctcgaccc ggcgcaatgg
    28681 aatgcgctcc tggcgcggca gcgggcgccc agccctttca tgcggcacga atacctggcc
    28741 gcgatggagt gcagcaagag cgccgagccg gccaccggct ggacaccccg cttcatcacg
    28801 ctgtggcggg aggaacagct ggaggcggcc tgcgcgctgt acctgaagga tcactcgtac
    28861 ggcgaatacg tattcgactg ggcctgggcc agcgcctacg aagagcatgg cctggcgtat
    28921 taccccaaag ccgtgattgc ggtcccgttc acgccggttc ccggcgcacg ccttctcgcg
    28981 cgcgacgcgg cggcgcggca ggcgctgatc agggccgtgc tcggctggtg cggccgggag
    29041 aagctctcgt cgctgcacct gctttttgcc gatgacgagg atgtggccgc ctgcgcggcc
    29101 gccggcctca tgttgcgcca cactgtccag ttccactgga tgaataactc ctggcgggac
    29161 ttcgaggcct tcctggccag cctggcgcac gacaagcgca agaagattcg ccaggagcgg
    29221 cgcaaagtgg ccgacgctgg cgtcgtgttt cgctggtccg aaggccgtga catcgataag
    29281 gccgactggg atttcttcta ccactgctac gagcgcacct accgcgagca cggcaacgcg
    29341 ccctacctca cacgcgactt cttccgccgc atggcgctgg acatgcccga gaactggctg
    29401 ctgttcatgg ccgagcgcgg ccaaaggccg attgccgcca gcctgatcgc actgggaaag
    29461 cagcccgggc cccagccgcc gcacgacggg aaagcggcgt acggccgcta ttggggcgcg
    29521 ctcgagcggg tggattgcct gcatttcgag gcctgctatt accagcccct gcagtggtgc
    29581 atcgagcacg gctacgaccg cttcgaaggc ggcgcgcagg gcgaacacaa gatggcgcgg
    29641 gccctgatgc ccgtcaagac gaccagcgtc cattggctgg cgcatccttc attcgcctac
    29701 gcggtcgatc gcttcctgca gcgcgagggc cagggcattg gcagctacct ggaacacctg
    29761 gagcgccgca atccgttcag gcagcgttga gtgttgcccc tagccagtgg tcccgtcagc
    29821 gaccgccggc cggcgcgcca tgggttaacg tccgtcctcg gagacacgac accatgaaat
    29881 ccctgagcgg cctggacgcc acgttcctgt acctggagac gcccgagacc ccgatgcacg
    29941 tcggctcgct gaacctgtac gagctgccca agggcttcaa gggcagcttc cataaagccg
    30001 tgcgccagca tatcgccaag cgggcgcacc tggcgcccat cttcagccgg cgcctggcct
    30061 tcatgccttt cgacctcggc catccggtct gggtggaaac cgatcggctg gacctggact
    30121 ttcacatccg caaggtgccc ggcaagcggc tcaccgtcag gcaggtcgag gcggccgcgg
    30181 ccaggctgca cggccagctg atcgatcgcg aacacccgct gtgggagttc tacgttttcg
    30241 acgacatcga ggcgcccgcg gccatgggga tccagggcaa gctggtgggc ttctattcca
    30301 agatccacca tgcggcgctc gacggcaagg gcggcaccgt gctggccaac gcgatactgg
    30361 atctcagcgc caccccgcgc gaggtcgcgc cggccgaccc gtcgcgcaag ggacccaggg
    30421 ccggagacct gaagatcggc gagatgatcg gagcggtctt ctccaactcg ctggcgcaat
    30481 acgccaagct ggcccggtcg ctgccggctg ccgccacttc gatcggcggc gccgtggccc
    30541 ggcagtcggt cggcggcggc gagaagggca tcaccagcct gcggcccaag tcgccgatca
    30601 agctggcgcc caggacgatt ttcaacgtcg gcatcacccg ccagcgtgtg ttcgcgacgg
    30661 ccagcttgcc ctttgccgaa tcgaaggcca tggccaaggc ggcgggaggc accttcaacg
    30721 atatcgtgct gtggatctgc tccaccgcgc tgcgcagcta cctggccagg cacgccacgc
    30781 tgccgaagaa gccgctggtc gccgccatgc cggtcagcct gcgcgaggag agcaacaagg
    30841 agctgaacaa ccaggcctcg atcaccgtcg tggatctggg aacccacctg gccgatccca
    30901 tcaagcgcat gaacgcgatc atcgcgtcca cggccaaggt caaggaggcg ctcgccaaac
    30961 tcaaatcggt gctgccgacc gattacccct ccctgctgtc gccgtggctg atgggcgggg
    31021 cgggcaaggc cctgttcagg acctacggca agggcggcat cgccggcagg ctgcccgcgg
    31081 tagcgaacct ggcgatcagc aatgtcccgg gcccccaagt gccgttgtac ctggccggcg
    31141 cgcgcatggt gacgttccat ccgctgtcca tcgtgctgca tggcctggca ctgaacatca
    31201 ccgtgcagac ctatgccggc cgggtcgatt tcggcatcat cgccgacaag caggcggtgc
    31261 cgcatgtgca ggacctggcc aatgcgctgg agaccgcgtt cgaggaggcc aaaggcctgt
    31321 tcctgcccgg cgcaacgccc atgcccgcga acccatcgac gacggcgcgc aagccgcgcc
    31381 gcaaaaccgc caccgcgagc tcgaagcgcc agtcgcgact atcctcggcc catgcgtagt
    31441 tccacaccca atcacgacct tcctgccccc agcctggccc tgctcgcgct cgaattcagg
    31501 gcgccgctgg agttcggctg cgttctgccc gcctggcccg cgttgcagcg cgcgcccgcg
    31561 ggcgacgggc acaccgtcct ggtgttcccc gggctttccg ccagcgacgc cacgaccttt
    31621 ccgctgcgcg gttacctcaa ctcgctcggt taccagaccc gcggctggag ccagggcttc
    31681 aatttcggtc cgcgggccgg cgtgctcgag gccgccggac gcgggctcga gcaggcctgc
    31741 gatacgagcg gccgcaaggt cagcctgatc ggctggagcc tgggtggcgt ctatgcgcgt
    31801 gagctggcca aggcgatgcc ggaccatgtc cgctgcgtga tcaccttggg ttcaccgttc
    31861 gccggaccac ccaagtcgac caacgcctgg cgcatctacg agttgaccag cggccgcgac
    31921 atcgagcgcg agcacggccg cttcgacctg ccgacggcgc cgccgcatcc gaccaccagc
    31981 atctattcgc gcagcgacgg catcgtggcg tggcgcggta gcatccaaca gccgcaccac
    32041 gctcacaccg agaacgtgga ggtcgtggcc agccacatcg gccttggcct caacccgagc
    32101 acctggtggg ccgttgccga ccgcctggcc cagcccgaag ggcagtggcg cccgttcgag
    32161 cgcagcggcc tgttcggctt gaagggattg atctaccccg acccgaaccg cgattgagtg
    32221 gttccattct tgtcatgccg ggctcgaccc gccatccatg acttcctgga ttgcggcgcg
    32281 gggccggagc ggccatgcct cggcacgtgc atagcgtagg cccggcattg ggctcgctgc
    32341 actggacagc tgcgcgcccc gggcgttccg cgagcgccgg gccggagcgt gatgcctcgg
    32401 cacgtgcatg gcgcaggcct gcaatcaata actatgctcc atgctcccgc tggtagctcg
    32461 cgatgccgtc catgatttcc ttgcgggcgg cgtccggccc ttcccaaccg aggatcttga
    32521 cccacttgcc gggctccagg tccttgtagt gctcgaagaa gtgcgcaatc gtcttcaggc
    32581 gcagcgggtt caggtcctcc ggctgctgcc agccgctgta gatcgcgagg atcttgtcgg
    32641 tgggcaccgc cagcaccttg ccgtcctggc cggcttcgtc ttccatcatc aggatgccca
    32701 agggcctgca ggtcaccacc acgcccggga tcaagggcac cggtgtgatc accagcacgt
    32761 ccacgggatc gccgtcgccg ctgagggtct tggggacata gccgtagttg gtggggtaat
    32821 gcatggacgt gctcatgaag cggtccacga agatggcgcc cgtttccttg tccacctcgt
    32881 acttcacggg gtcggcgttc atcgggattt cgatgatcac attgaaggcg tcgggaacgt
    32941 gcttgccggg ggtgacttgg tcaagtgaca ttgttttcgt tacgtcaatg aaaggtgaaa
    33001 taaatcccgg tattaaccct gattttactt tcaccagtct tgcttgccca tcagcggcga
    33061 atcggcatag ggtctgccct tatatactcc tgcggttggc caatcgtctc cgccccgcct
    33121 gaagggggct acgaggaagc aacgcagcgg aggcaccgct cgcggggcgc ctctagcacg
    33181 ttactcaatc gaaacaacgt aggagaatca aaaatgacag gcaattcggc gctgatactg
    33241 gcgctcgtct gcggcctcgc ggccgtggtg tacgggttct gggcccgcgg atggattctt
    33301 tcccaggacc ccggcaatcc ccggatgcag gagatcgcgg ccgccatcca ggccggcgcg
    33361 ggggcgtacc tggcgcgcca gtacaagacc atcggcatgg cgggcgtggt gctcgccgtc
    33421 ctgatcggcg tcttcctcga cacgaccacg gccatcggct tcatcatcgg cgccctgctg
    33481 tcgggtgcct gcggctttat cgggatgaac gtctcggtgc gggccaacgt gcgcaccgcg
    33541 caggcagcca ctcgcggcat cggcccggcg ctcgacgtcg cgttccgcgg cggcgccatc
    33601 accggcatgc tggtggtggg cctgggcctg ctgggcgtgg ccgggttcta ctggttcctg
    33661 gtcggcaacg gcgtgctgac gccggaccgc aagctgtcct cgctgctcaa tccgctgatc
    33721 ggcttcgcat tcggtgcctc gctgatctcg atcttcgcgc ggctgggcgg cggcatcttc
    33781 accaagggcg ccgacgtggg agccgacctg gtgggcaagg tggaagccgg catccccgag
    33841 gacgaccccc gcaacccggc ggtgatcgcc gacaacgtgg gcgacaacgt cggcgactgc
    33901 gccggcatgg ctgccgacct gttcgagacc tacgccgtta cgctgatcgc cacgatggtg
    33961 ctgggttccc tgctggtcgc cggttatccg accaatgcgg tgctgtatcc gctggcgctg
    34021 ggcggcgtgt cgatcatcgc ctccatcatc ggcaccttct tcgtgaaggc ctcgccgggc
    34081 atgaagaacg tgatgcctgc cctgtacaag ggcctggcga tcgcaggcgt gctgtcgctg
    34141 atcgccttct actttgtgac ggtgtggctg atgcccgaca acgcgatcac cgcgaccggc
    34201 acccagatgc gcctgttcgg cgcctgcgcc gtcggcctgg tcctcacggg catgctggtc
    34261 tggatcaccg agttctacac cggcacccag tactcgccgg tgcgccacat cgccgaggcg
    34321 tccaccacgg gccacggcac caacatcatc gccggcctgg gcgtctcgat gcgttccacc
    34381 gcctggccgg tgctgttcgt ctgcatggcg atcctggttt ccttctggct cgccggcctg
    34441 tacggcatcg cggtcgccgc gacctccatg ctgagcatgg cgggcatcgt ggtggccctg
    34501 gacgcctatg gcccgatcac cgacaacgcc ggcggcatcg ccgaaatggc ggacctgcct
    34561 tcgagcgtgc gcgacatcac cgaccccctg gacgccgtgg gcaacacgac caaggccgtg
    34621 accaagggct atgccatcgg ctccgccggc ctggccgcgc tggtgctgtt cgccgactac
    34681 acccacaaac tggaaagcta tggcagttcg atcagcttcg acctgtcgga cccgatggtg
    34741 atcgtgggcc tgttcatcgg cggcctgatc ccctacctgt tcggcgccat ggccatggaa
    34801 gccgtgggcc gcgccgcggg cgcggtggtg gtggaagtgc gtcggcagtt ccgcgacatc
    34861 aagggcatca tggaaggcac ggccaagcct gaatacggtg ttgccgtgga catgctgacc
    34921 aaggcggcga tcaaggaaat ggtgatcccg tcgctgctgc ccgtggtcgt gccgatcgtg
    34981 gtcggcctct tgctgggccc gaaggccctg ggcggcctgc tgatgggaac cattgtgacc
    35041 ggcctgttcg tggcgatctc catgtgcacc ggcggcggag cctgggacaa cgccaagaag
    35101 tacatcgagg acggcaacca tggcggcaag ggttcggaag cgcacaaggc ggccgtcacc
    35161 ggcgacacgg tgggcgaccc ctacaaggac acggccggcc ccgccatcaa cccgctgatc
    35221 aagatcatca acatcgtggc cctgctcatc gtgccgctgg tggtgcagtt ccacggagtc
    35281 caggcgccgg ccaccaaggc tgaagcaccg gccgcggtga tcgctgccgg caccacgccc
    35341 gttcacgccg gcgccgtgac ggccgcacct gccaaatagt cgttccgcgc cgagagaaat
    35401 gaggcccgcg tcgcgggcct tttttcttgc cccgccggag cccgatgccg gattcgccgg
    35461 atgcgccttc aggcgccggt ctcgatgacg gcgcgaagca gctggctgga aggctccacc
    35521 agttccggca gccgcgccac acggtcctgc tcgatgtatt gcaggatcag ctcgttgatg
    35581 ccgcccacca ccgccatggc cattccgggc gacagtggcg tcgtgcgccg gtggccttgc
    35641 tcgcgattca ccacgctgag catgaagtcg gcgatctgcc ggttgacacg ccgccttgcc
    35701 gccagccctt cggtcccgag acccagaatt tcgatgaaca gcgtgcgcat cagcaccggg
    35761 ttttgcgcca tgcagcccag gtacgcggtc agcgcgcgct cgacttgtgt ctgccattcc
    35821 tgctgcggat cgatcgaatc gcgcagcacc ttcagcgcat tgcggctggc ggcctcatac
    35881 agcgcgatca ggcactcggc cttggtcgag aagtgctggt agaaggtgcg gcgcgagacg
    35941 ctggcttcgc gcacgatgtc ggcgatcgtc gtgtccgcgt aacccttgga cgcgacggcg
    36001 cgcgccatgc cgtcgagcag ccgatgcagg tgttcgcgga cttcggttcg ggtttccgtt
    36061 tcaaccatgg cgcctatttt cagcctgttg ccagccggga cttgaaaatg ccggtcaacg
    36121 gacgatgatt tcggcggtac catgaagtac cagccttgac cgcgtgcttc ccatgaccaa
    36181 cctcgtcgtc cgccgcctcc tgatcgacat ggaagccccc attgcccgtc actggtgcgg
    36241 cggtgatgcc tttcgcaccg ccctgttcaa cgccttgtcc atgagtttcc cggtgggcga
    36301 gcaattcttc atcgatgcgg tgcgcaacgg cttcaagggc ctgcccgcgg acaagcagga
    36361 gcgctaccgc gccgaagtcc agggcttcgt cggccaggag gccacccacc gcaggctgca
    36421 cggcctttac aacgagcacc tggaaagaca gggcctggtc aacgactggg ctccgcgggc
    36481 ggcgcggcgc ctgaagcagc tggaagggct ggatccgcgc cacgcgttgg cgatcacggc
    36541 cgccaatgag catttcacgg ccatcatggc gagctggatg cttcacaatc ccgacctgct
    36601 cggcggggaa gacccgcgcc tggccacgct gtggctgtgg catagctgcg aggagtccga
    36661 gcacaagagc acggcgttcg atctctacca ggcactggac ggcaaccacg agtggcgtat
    36721 cgcctggttt cgccgcgtca cgcggttctt tctcgcggat accctgcgcc agacggtgaa
    36781 caacctgcgc cgggacggca cgctctggaa atggagcacg tggaaaagcg cggcttctta
    36841 cctgttcggc aagcgcggac tgatacggat gacctacagg ccctggcgcg actatctgcg
    36901 gcgcgatttc catcccagcc agcaggacgc cggcgcgtcg cagcggtggc tcgccgagca
    36961 tcgcggcgag tacgtggcgg tcggcggttc cgcctaggcg cgggggaagc ctgcgcgtct
    37021 ttcaagcaga atgttggcat gcaactccat tacatcgcca acacggctca agcctctttc
    37081 tccggccgca cgatccccgt gatcgatccg tccgacgggc aggccttcga cgaactgcaa
    37141 cgcggcaatg ccgaggacat cacggccgca gtgcgcgcgg cgcgccggtg ttacgaggcc
    37201 gtctggagca agctcagcgc cgccgagcgc ggccgcctgc tgatgcggct gtcgaacaag
    37261 atcgccgagc atgccgaaga gctcgccacg ctggagcagc gcgactgcgg caagcccacc
    37321 aagcaggcgc acgccgatgc ggtggcgctg gtgcgctatt tcgagttcta cgccggtgcc
    37381 tgcgacaagc tgcacggcga caccctgccc tacctggacg gctacagcgt gttcacctgg
    37441 cgcgagccgc acggcgtgac cggccacatc attccgtgga actacccgat gcagatcttc
    37501 ggccgcagcg tgggcggcgc gttggcggcg ggcaacgtgt gcgtggtcaa accctccgag
    37561 gacgcctgcc tctcgctgat ccgggtcgcg cagctcgcgg ccgaggtcgg ctttcccgcg
    37621 ggcgccatca acatcgtcac cggctacggc catgaagtgg gcgacgccct ggcgcggcac
    37681 gaaggtatcg accacatcag tttcaccggc agcccgaaag tcggaacggt gatccagcag
    37741 gtcgcggccg agcgccactg cccggtcacg ctggagctgg gcggcaagag cccgcaggtc
    37801 atcttcgccg acgcggacct ggacgcggcc cttcccatgg tcatcaatgc gatcgtgcag
    37861 aacgcgggcc agacctgctc ggccggctcg cggctgctgg tggagcaggc gatctacgag
    37921 ccgttcctgg aacggctggg caaggccttc gaaaacctgc gcgtcggccc cgccaccatg
    37981 gacctcgacg tcggcccttt gattcgcgcc agccagcaac agcgcgtctg ggacttcctg
    38041 tccgacgcgc acgtggccgg catcccaacc gtggcgcaag gccaggtggt ggaggaagct
    38101 ccggagaccg ggtactacca ggcgcccatc ctgttgcgtg atgtgcccgt cgatcaccgg
    38161 gtggcgcagg aggaggtttt cggccccgta ctcgcggcca tgagcttcaa ggacgaggac
    38221 caggcggtcc agatggccaa tgccaccagg ttcgggctcg ttgccggcgt gtggacacgc
    38281 gacggcgcgc gtcagcttcg catggccaag cgcgtcagga gcggccaggt cttcatcaac
    38341 aactacggcg cgggcggcgg cgtggagctg cctttcggcg gcgtcaagtc atcgggctac
    38401 ggccgcgaaa aaggattcga ggccctgtac ggcttcacca cgctgaagac ggtggcgatc
    38461 cggcacgggt gacggtttgt cattgcgggc cgcaatccag gaagtcatgg atgccgggtc
    38521 gagcccggca tgacaggccc atagtcaacc cctgatccgc gctgtcttca ccacggcctc
    38581 ggccatgggc agcagcttct cgaagaaagc cagccgcggc gcggcgaaca tcgcggcata
    38641 cagctcgtcc ttgtgcacgg ccacccaacc gacgcccttg agcgtcacat cgtcggcacg
    38701 ccgggccagg gtgaattcga agcgcacgcc ctttgcgccc gcgaacacgg tgggctcgac
    38761 gcgggtcacg ttgaccacgc cttcgttcgc atagagcgct tcgaacaagc tgaccagctt
    38821 gtccggcggc aagccggccg tgaaagtcgg atagcgggcg tccttctcgc ccggcgcacg
    38881 gaacagcacc gtcggcttgg cgaccagcgc cttgcccgac cggataccgg cccacaggcg
    38941 caggtggtcc agcggaatgc cttcctgcgt ccacatgtcg tagggcccgg cttcccacgg
    39001 atccgtcacc ttgttccagg catcggacac gttgaccacc agcttgccat tgacgacctg
    39061 gtcgccttcg accttggcaa tggaagtgca tgcggccatc agaaaggcca gcgccagcgc
    39121 gagaatcgtc tttgtcatgg cttcaattcg gaaagatagg ctttgagcaa gccggcatcc
    39181 ggcgcatccg gcgacagggc caggtatttt tcgaacgctt gtaccgccgc cggcgcatcg
    39241 gaacgctgct tgtggaccag gcccagcgaa cggaaggctt caaccggggg cgtctccacg
    39301 gccgtcgcgc gcgccaggtc gtccagcgaa cggggcaggt cgtccttgtc tccgcgcagc
    39361 cggtacacct cgccccgggc gtaaagaacc tccgcgtccg agggcttgcc ggtcagcagc
    39421 cggtcgaaca gcaccaggct ttcctcgtac tggccgcgct tgacttcgtc ctgcagccag
    39481 ccgtagcgca gcggcgcgat cgccttctgg tactcggccg cggcggtggc gccgcccgcg
    39541 tcgcccgcca gctcgagcag gtcgtcgcgg cggttgcccg cgggcggatg ggtggcgaac
    39601 atcgcgctgc gctcgccgac gttctctccg cccgtgatct tgagttcgcc aagcaggttg
    39661 tcccagacca ggggcgcctg gcggccctcg tagccggctc ccttcatgag gcgcatgccg
    39721 agccgatccg ccctcgtctc atgctggcgc gagaaagcga actgcgtcgc caggatgccg
    39781 atcttgccga ccgcgccggc aatgcccccg atgaggccga cgatctgggc gagcatcgcc
    39841 ttgtccttgg catcgcgcag ttgctccagc tgatgccgct ccaggtaatg gcccagctcg
    39901 tggccgacga tggcggccag ctgggcttcg ttttccatgc gcagcagcag cccgctccat
    39961 acctgcatca tgccgttggg cgccatgctc gcgttgaaca tcggcgtgcg catcacgtga
    40021 acgcggatgt cggggcaatg gccgtcggtc agccggcagg tcacgtccga caggtacttg
    40081 cccagggcgg ggtcccggat cgccaggggg ctgcggcgca gcttggcctc ttcgcgatcc
    40141 atcaggttcc acagaccgcc ctcttccgta tccaccgccg gccggctgaa gcggcccggc
    40201 accttgaagc tgcccgacgg cgccgcgtcc tgcgccacgg ccggcaggcc ggacagcaac
    40261 gcgccgaatc caaggcaatg cctgcaggcc aacttgagaa agccccggcg ggcgtcaggc
    40321 gcgttcattg cgttcccctt tgccgccgcg gcggttccga gcggcgctca ttgcaacacc
    40381 gggaagccct cgagcagggt ctccaccgtt tccatggcgg ccttctcgtc gcgcatgtca
    40441 cccgacatgc ggcgcaggtc gttgaaccac acgacgcgcc cgctgttcag gtccaccagg
    40501 gatgcatagc caatctgctc gccgccgaag ctgatcgccc cgagcagcgc catggcgatc
    40561 atcgtggcct tgcgctcgtc actggcgtag ctgtcgcgga tccaggtgaa cagcgcatag
    40621 tcggcgccgg tcctggcttt cagcggcttt acggcctcgc ccagcgacca gtccaggcgc
    40681 tcgttcttgg tcggcagctt catgcccatg ccggtgtggt gaacgaaaac ggcttccgcc
    40741 acggcccgtt gcagggcatt gagttcggag aattcgtcca gctcggcgtc ctgcagttcc
    40801 acggcgttcg cacccagctg gtcgcgccgg acggccagcg ccgccttgaa attcctctgg
    40861 gctgcctcgg tccagtcggc cttgggttcc agcacgccgc cggcgctgat ggaaaacagc
    40921 tccatgtcgg ccggaacgat cacgagtttg gacccggcag ggcgggcgtg aaatccgggc
    40981 gcgaggtgct tgggatcgga ctcctgcgcc tggagggcgc cggcgaacag cacgacggcc
    41041 agaacaatac tgcttcgaaa caacttgggc atggtacttc agcctctggg cctgcattct
    41101 ggcgcgccgg ccgatttgac gaacccggct aaacccgaat ccgaaatcag caacaatggc
    41161 tggcttgagc gcgcgcaagg aaatgggttc ggcgctgggc ggttcgcttg cagatgggcc
    41221 gccgcaacgg ccggatttca cgaaggcgtt ctcttcaagc aactcatcag ttacggccgg
    41281 gtccggcttc cggcaacgcc gcagccactt tcgttcgaac aaggagttat cgcgatgcgc
    41341 gtccaggaca aatccatcat cgtcaccggc tcgggcggcg gcatcggcga aggcatcgcc
    41401 aggcggctgg ccgccgaggg cgcgaatgtc atcgtcaacg acatcaacgc ggccatgggt
    41461 gaaaaagtcg tcgcgcagat caaggccgcg ggcggcatcg cgtccttttt cgccgccgac
    41521 gtcacgaagt ccgccgacgt gaaggcgctg gtgcaggccg ccgtgacgcg ccacggcaag
    41581 ctcgacgtga tggtcaacaa cgcgggctgg acccatcgca accggccggc gctggaggtg
    41641 agcgaggagg agttcgacaa gtgcttcgcg gtgaacatga agagcatcta cctgtcgacg
    41701 atccatgccg tgcccgcgct gcgcgcgaac aagggcggca gtttcatcaa catcgcctcc
    41761 accgcagggg ttcgcccgcg gcccgggctg acctggtaca acggctccaa gggcgccgtg
    41821 atcaccacca gcaagtcgct cgcggccgag ctcggtcccg acaacatccg tgtgaactgc
    41881 atcaacccgg tgttcaaccc cgataccggc ctgtcggcgc aattcgcggg cggccccgtg
    41941 gatgaggccc gccgcgccaa gttcctggcg accattccgc tcggccgttt ctcgacggcg
    42001 ctggatgtgg ccaacgccgc gctttacctg gccagcgacg aagccgcttt catctcgggg
    42061 gtttgcatcg aggtggacgg cgccagatgc gtctagggcc acccgacccc aaagtattcc
    42121 gggattagtt cctgcgcgac aatcggggta tgagccaacg cttcattccg ttgatcgacc
    42181 agcgcttcgc ggcggtggcc gcccgcgttc ccgcgcaatc aattcccgcg accggcctgc
    42241 tcgccgacag cctgggccgg ccgctgcgcg acctgcgcat cagcgtcacg gaccgctgca
    42301 acttccgctg cagctactgc atgcccaagg aagtgttcga caagaaccac ccgtacctgc
    42361 cgcattcggc cctgttgacc ttcgaggaaa tcacccggat agccaggcag ttcgcggccc
    42421 atggggtgcg caagatccgc ttgaccggcg gcgagccgct gctgcgaaag gacatcgagt
    42481 cgctgatcga gcagctggct gcgctgcgca cggtggacgg tgagcccctg gacctgacct
    42541 tgaccacgaa tggctcgctg ctggcccgaa aggccaaggc cctcaaggcg gcggggctgc
    42601 agcgtgtcac ggtcagcctc gacgggctcg acgacgcggt gttccggcgc atgaacgacg
    42661 tcgattttcc ggtggccgaa gtcctgcagg gtatagctgc ggcgcatgaa gcgggactcg
    42721 ggccgatcaa gatcaacatg gtggtcaagc gcggcaccaa cgagcatgag atcctgccga
    42781 tggcacgcca tttcaagggc accggcatcg tgctgcggtt catcgaatac atggacgtgg
    42841 gcgcgaccaa cggctggcgc atggacgagg tgctgccctc ggccgaggtc gtgaaaatga
    42901 tccaggccca gctgccgctg gttcagctgg agcccagcgc cccaggcgaa accgccgagc
    42961 gttggcgcta cgccgacggc ggcggcgaga tcggcgtgat cagcagcgtc acgcaggcgt
    43021 tctgcggcga ctgcaaccgc gcgcggctgt ccaccgaggg ccagctgtac ctgtgcctgt
    43081 tcgcgaccca gggccatgat ctgcggtcgc tggtgcgcgg caccggcgcg aacgccgccg
    43141 gtagtgccag cgacgagcaa ctggcgtccg cgatagcccg catctggcag ggccgggccg
    43201 accgctattc gcagctgcgc gggaccatgg cgcccgatac cgacaccggc gggcgccggg
    43261 tcgagatgag ctacatcgga ggctgaatga acccaccccc gaagcggcct ccggccgcct
    43321 ccccctcaag ggggcgccac cagcggcccg gcaaagccgg atccgcggtg gcccgcgaat
    43381 tgcgcaacgt agcgcaacgg gatcttcgca gcgcctgtag aactccatga ttgatccctc
    43441 gctgataacc ggtgtgatcc tcgccggcgg ccgcggatcg cgcatgggcg gcgtcgacaa
    43501 aggcttgcag aatttcaacg gcatgcccat ggcgctgcac acgctgatgc ggctggcgcc
    43561 gcaagtcggc gaggtgatga tcaacgccaa ccgcaacctc ggcgcgtatg aatcgttcgg
    43621 cgtgccggtc tggcccgacg tcctggccga ctatgccggg ccgctcgccg gttttctcac
    43681 agcgatcgag cgctgcgaaa cgccatggct cgtcacggtg ccgtgcgaca cgccgctgtt
    43741 cccccaggac ctggtcgccc ggctcgcgcg ggccgccgag gaacaggata ccgagatcgc
    43801 gatggccgcc gcgcgcgagg aagacggcca gatccgcgcc cagcccgtgt tctgcctgtt
    43861 gcgcgtgaac ctgctggaaa gcctggtcag attcacgcac ggcggcggcc gcaagatcga
    43921 cgcctggacc tcgcagcaca agacagtggt ggtgccgttc gaccagcctg gcgatgagcc
    43981 cagggccttc ttcaacgcca acacgctcgc cgagctgcac ctgctcgaac aggcgaaatg
    44041 aagacgctcc aggagatcgc ggcgcaactg cagggctacg acccgcaagc gctgagcgct
    44101 caggccgtca atgatttcct ggcgcggctg gtcgaacccg ttggcggcat cgagcacgtc
    44161 gatgtgttcg acgcgctgga cagggtgctg gcacaagaca tcgtttctcc catcagcgtc
    44221 ccgccccacg acaactcggc gatggacggc tatgccttcg acggcgcgca gctcaaggcc
    44281 accgaattgc tgaccctgga ggtcgcggga acggcatttg cgggcaaggc ctggcaaggc
    44341 tcggtggccc cgggccagtg cgtgaagatc atgaccggcg cgatcatgcc cgccggcacg
    44401 gacaccgtgg tgccgcagga attcatcgag gacaagggcg acggccggat ttccgtgccg
    44461 cccaagctgc tgcagcctgg cgacaaccgg cggctcaggg gcgaggacct gcacgaggga
    44521 caaccggctt tggcgcgcgg tgcgcggttg acgcctgctg cttgtggctt gatcgccagc
    44581 ttggggctga aggcagttgc cgtctaccgc cggttgcgtg tagcgtattt ttccacgggt
    44641 gacgagatcc tgagcctggg cgaagtgccg cgcgaaggcg ccgtgtacga cagcaaccgc
    44701 tacaccgtct ttggcctgct gactcggctg ggctgcgaag tcatcgacat gggcgtcgtt
    44761 cgcgacgatc cggcgctgct ggaagcggct ttccgcgagg cggccgcgaa agccgacgcc
    44821 atcgtcacca gcggcggcgt cagcgtcggc gaggcggact acacgaaggc gatgatgaag
    44881 aagctgggcg acgtggcatt ctggaagatc gccatgcggc ccggccggcc gatggccgtg
    44941 ggccgcatcg ggccggcgat cctgttcggc ttgccgggca atccggtggc cgtgatggtg
    45001 accttcctgg ccttcgtgcg tccggccctg ctgcgcatga tgggctgctc gagcgcgcag
    45061 ccgccgcagc tgaaggcccg cagtgaagaa ccgatccgca agaaagccgg ccgtaccgaa
    45121 taccagcgcg gcgtcgtcac tacggccgcc aacggctcat tgcaggtgcg caccacgggc
    45181 aaccagggat cgggggtgct cagctccatg gtccaggcca acgggctgat cgtgctgcat
    45241 cacgcgcagg gcaacgtggc tgccggagac ccggtcgacg tgatgatgtt cgaaggtgtg
    45301 atctagggcc tgctctggac gccgtctccg ccgatccgca gcacggcgat cggcggcagg
    45361 tcgcgcgaca ggcatcgcca ggacacgccc gcgtcctcgc tgacccacag gccaccggtg
    45421 gtcgagccca tcgccagcgt gcggccttct tttccggcca ccagcgcatg gcggtagatc
    45481 aggtgatagg cgtcgtgctg cggcaggcct tcgccatggc tcacgaagga agcgccgcca
    45541 tcgcgcgttt cattgaccac caatcgtcca tccgccggca tgcgctgcgc gtccgaatgg
    45601 gccggcacga accaggcgca ttgcgggtcc tgcggatgag ccgcgacggg aaagccgaag
    45661 ccgctgggtt gcggcgccgc gatggcttcc cacttcaggc cagcattcgt ggagcggtaa
    45721 aggccgccat ggtgctggca ccacaggaca tcgggctgcg cgaggcattg gtcgatgcgg
    45781 tgcgcgtcct ggatgttggg atcttcgcgc cgttccgggg gcatgaagtc cgccaccatg
    45841 cccgccgatg tattcgtcca gctggcgccg ccatcgcgcg tttgccagac gccgccgcaa
    45901 gagatcgcga ccgtgacatg agatggatcg cgcggatcga ccaggatgga gtggatgccg
    45961 gcgtagtcgt aaccaccgcc aatccagtcc ttgcgctcgg gccggtccca caggctttcg
    46021 accagctgcc agctggcacc accgtcgcgc gacgagaaca gaccggcagg caggcagcct
    46081 gcccacagcc ggccatcggg agaagcttcc agggcccaga cctggtccac cgtccagggt
    46141 gtcgcgtcgt ccttccatgg cccttccgtc ggcttgggcg ggaacgccgg cgcggccatt
    46201 tcctgccaac tggcgccgcg gtccaggctc ttgcgcagct tgatgccgaa gtgacccagc
    46261 cgcagggccg cataccaggc gccgtcgtac gggtcgacca ggacctgcgt gaccggctca
    46321 ccggcgaaat ggggcttgcc gagacgccag cccgagtcgc cgcggtgggc gatgaacaat
    46381 cccttgcggg tgccgatcca gagcgtggct gccatcgtta acctcctgtg agggcctgga
    46441 tgaccatgac cttgtcgccg ggcccgatcg caacgtccag gtcgctgcgg ctctcgatca
    46501 tccgcgcatt gacgaaaacc gctacgtgct ttcgcacggc tccctgctcg tcgagcacat
    46561 agccgcgcag agccggagcg gccaggaacg cggcgttcag ggcgtcccgc aatgtggcgg
    46621 cctggacatg ctgcggccgg caagcgacgt gccgtgtcag cgcgggtgcg aactcgacgg
    46681 tgggcatatt tgt
//
LOCUS       DASWIY010000083         1982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2637601, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000083 DASWIY010000000
VERSION     DASWIY010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2637601"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VGA59_03350"
     CDS             complement(<1..546)
                     /locus_tag="VGA59_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9718742.1"
                     /translation="MGSDNGQGAGRRQGAGPLQGLKVLDLTEHMAGPFCTMILADMGA
                     EVLKLERPGKGDSSRAMGDGSERNPYFRYINRNKKGLTLDYKQPAGREVFLRLAQSVD
                     VLVENYRPTVMQRAGLGYEALREVNPRLIYAQLSGFGYDGPYAGRGGFDLIAQGMGGI
                     MHVTGEPGGPPTSVGLPICDLG"
     gene            784..1974
                     /locus_tag="VGA59_03355"
     CDS             784..1974
                     /locus_tag="VGA59_03355"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX9718743.1"
                     /translation="MGAALVNSVSQAMHIMARRDDQSVFSDVTWQQESREAVANVLHG
                     RMAARVAAHLEDCRAQGIADRRNGSYTRHVLTTLGDLELEVPRTRTFNPSGLLAAYAR
                     RAPEIDRLILAAFLLGLSTRKVGQTLLRMLGESVSASTVSQVAKQIDAEVKAFHRRRL
                     KDPYRVLMFDGVVLARKSGAGALRRPVLVALGIRPDGRKEVIDFRLAKGESQAAWEAF
                     LNDLYRRGLTGQAARLVVSDGGAGLLAALPLVYPHLPVQRCWAHKVRNVLNKVKKGDR
                     DAVKRDLHRIMNAKGLPGARSAARRFAQRWQDAYPKAVACLRGDLDELLVHLEVFRDA
                     DWRKAARTTNAIERCFVEIRRRTRPMGVMADRASCERIVYAVFTGINQAQGTASPFPL
                     TQNS"
BASE COUNT          301 a          681 c          692 g          308 t
ORIGIN      
        1 gcccagatcg cagatcggca gacccaccga ggtcggcggg ccgccgggct cgccggtcac
       61 gtgcatgatg ccgcccatgc cctgcgcgat gaggtcgaat ccgccgcgcc cggcgtaggg
      121 cccgtcgtag ccgaagccgg agagctgggc gtagatcagg cgcgggttga cctcgcgcag
      181 ggcctcgtag cccagcccgg cgcgctgcat cacggtgggg cggtagttct ccaccagcac
      241 gtccacggac tgcgccagcc tgaggaagac ttcgcgcccg gcgggctgct tgtaatccag
      301 cgtgaggccc ttcttgttgc ggttgatgta gcggaagtac gggttgcgct cgctgccgtc
      361 gcccatggcc cgcgaggagt cccccttgcc cgggcgctcc agcttgagca cctccgcgcc
      421 catgtccgcc aggatcatgg tgcagaacgg ccccgccatg tgctcggtca ggtccagcac
      481 cttcaggccc tgcaaggggc cggcgccctg gcggcggcct gcaccctgtc cgttgtcgct
      541 gcccacggcc ctcctctgtc ctctcggtgt gaagtccctc gcgccccggc cggcgggggc
      601 tccgcgccct cggccgctgc gattcggccg cgcgtgcgcg gctccctctt agtctaacgc
      661 cgcccggccg cgttggaaac ctgcccgcgg ggggaggtga cgtacgaggt cttgtgtcag
      721 gggatgaaaa cgggggaact ccttgggtgt acgcagtgaa ccttcgacca aggagaaccc
      781 ccgatgggag cagcactggt gaacagcgtg tcccaggcca tgcatatcat ggcgaggcgg
      841 gacgaccaaa gcgtgttcag cgatgtgacc tggcagcagg agagccgcga ggcggtggcg
      901 aacgttctgc acgggcgcat ggcggcccgg gtggcagcgc acctggagga ctgtcgcgcg
      961 caaggcatcg cggaccggcg caacggcagc tacacccgcc acgtgctcac gacgctgggc
     1021 gacctggaac tggaggtgcc gcgcacgcgc accttcaatc cgtccgggct gctggccgcc
     1081 tatgccaggc gcgccccgga gatcgaccgg ctgatcctgg ccgccttcct gctgggcctg
     1141 tccacgcgca aggtgggcca gacgctgctg cgcatgctgg gcgagtcggt aagtgcatcc
     1201 acggtgagcc aggtcgccaa gcagatcgat gcagaggtca aggcctttca caggcgccgc
     1261 ttgaaggacc cgtatcgcgt gctgatgttc gacggggtgg tgctggcccg caagagcggc
     1321 gccggggccc tgcgccgtcc ggtgctggtg gcgcttggca ttcgccccga cggccgcaag
     1381 gaggtgatcg acttccgttt ggccaagggc gaatcgcagg cggcctggga ggccttcttg
     1441 aacgacctgt accgccgcgg gctcaccggc caggcggcgc ggctcgtggt cagcgatggc
     1501 ggtgccggcc tgttggccgc cctgccgctg gtctacccgc acctgcccgt gcagcgctgc
     1561 tgggcgcaca aggtgcgcaa cgtgctgaac aaggtgaaaa aaggcgatcg cgacgcggtc
     1621 aagcgcgacc tgcaccgcat catgaacgcc aagggtctgc ccggcgcacg cagcgcagcg
     1681 cgccgcttcg cccagcgctg gcaggatgcc tatcccaagg ctgtagcctg cctgcgcggc
     1741 gatctggacg agctgctggt gcacctggag gtgttccggg atgcggactg gcgcaaggcc
     1801 gcccgcacca ccaatgccat cgagcgctgc ttcgtggaga tccgccgccg cacccggccc
     1861 atgggcgtca tggccgacag ggccagttgc gagcgcatcg tctacgccgt gttcacaggc
     1921 atcaaccagg cccagggaac ggcctctccg ttccccctga cacaaaactc ttgacgtgac
     1981 cg
//
LOCUS       DASWIY010000084         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1161461, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000084 DASWIY010000000
VERSION     DASWIY010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161461"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="VGA59_03360"
     CDS             <1..163
                     /locus_tag="VGA59_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX9718744.1"
                     /translation="RPPAAPRDPFFDQPYQPPVTEAPPAWEAAVKPATRASANIKPKR
                     KVAALFKSE"
     gene            complement(328..1158)
                     /locus_tag="VGA59_03365"
     CDS             complement(328..1158)
                     /locus_tag="VGA59_03365"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEX9718745.1"
                     /translation="MALYLIGDVQGCDAALGRLLEKIEFSASRDTLYMLGDLVNRGPE
                     SGAVLRRLMAFGDAACCLLGNHDLSLLAVAHGRRAPHGNDTMDKVLLARDRGELLEWL
                     RWQRMAIRAHGLLMVHGGVLPQWDVAQTLMLAAEVEATLRGPDLVDFLSQMYGNKPAQ
                     WDDSLKGADRLRVIVNALTRLRFCTPEGVMDLKAPGGPRDAPPGYLPWFDVPGRKTAD
                     ATIAFGHWSTLGFLQRPDIISLDTGCVWGGALSALRLGAPGARHELIQVKCEQVRVPG
                     "
     gene            1183..2502
                     /locus_tag="VGA59_03370"
     CDS             1183..2502
                     /locus_tag="VGA59_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX9718746.1"
                     /translation="MEVLLIALLTLLNGVFAMSELALASSRKARLVLLAEDGDAGAAA
                     ALKLMDQPTRFLSTVQVGITSIGVLNGIIGEAAFSDGLANWLQILGLGPRASAYTATG
                     IVVALITYITIIFGELVPKRIGQLYPEAVSRLLAQPMTWLARVAGPFVKLLSASTQGV
                     LKLLRIDTTQPRGMTEEEIAHSLEEGVDAGVIEQHERQMVANVFHLDNRPLTSMMVPR
                     SDVQWLDASLGIAQALREIGEGGAHSWYPVCRGSLDNVLGVVSVARLLALGPDPAGTV
                     EQHATPALFVPETLTGMELLEQFRARSGRMVFVVDEWGVVQGLMTPHDLLEAITGELQ
                     PVAQAEAWATRREDGSWLLDGQMPVAELKARLDIRELPEEERGRYNTLAGLLMSVSGR
                     LPATGERIGCGDWMFEVIDLDGKRIDKVLALPLQADAQLRARRGTAA"
     gene            2522..3268
                     /locus_tag="VGA59_03375"
     CDS             2522..3268
                     /locus_tag="VGA59_03375"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEX9718747.1"
                     /translation="MSDAVYYEKPVLLDRDRHRKLRVGASQDFGFAGKANSLYLAGVE
                     FAEACKEYAIVFSRAVNGKIAPVAMLGLRGRENLFVDDDGRWTGTYVPAFVRRYPFVL
                     AELSGESMGVCIDEAFKGLGDSRGQALFDDQGNNTVFLQHALDFLNRFQQEYRRTEIF
                     CERLERAGLLKQMDARADLVDGRTFTVRGLLVVDEPKLMALPDATVLSLFRAGELHLV
                     SMHLVSLANMRQLVDRMARRGIPPPATTPG"
     gene            complement(3265..>3451)
                     /locus_tag="VGA59_03380"
     CDS             complement(3265..>3451)
                     /locus_tag="VGA59_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718748.1"
                     /translation="VFRALSVMSTRPINRHWILAHSKLRAEQVDKLLQRLVEEGAVEV
                     IDGSKFAVGADPHRGLH"
BASE COUNT          569 a         1154 c         1166 g          562 t
ORIGIN      
        1 ccgtccgccg gccgcgcctc gtgacccgtt cttcgaccaa ccctaccagc cgcccgtcac
       61 cgaggcgccg ccggcctggg aagcggccgt caagcccgcc acccgcgcct cggccaacat
      121 caagccaaag cgcaaggtcg cggcattgtt caagagcgag tgaagcttgc agtggctgtc
      181 atcccgggcg agcccgcaat gaccatatca ttgtcatccc ggacttgatc cgggatccat
      241 gacttcagaa ttcatggatt gcgggtcaag cccgcaatga caggggctgg tagattgggg
      301 gtcgagcgcg caatgacaat gtcattgtca tcccggcacc cgcacctgct cgcatttcac
      361 ctggatcagt tcatgacggg cgccgggcgc gcccagccgc agcgcgctca acgcaccgcc
      421 ccatacacaa cccgtgtcca gcgaaatgat gtcgggccgc tgcaggaagc ccagcgtcga
      481 ccagtgcccg aaggcgatcg tcgcgtcggc ggtcttgcgg cccggcacgt cgaaccaggg
      541 caggtagccg ggcggcgcat cgcgcgggcc gcccggggcc ttgaggtcca tcacgccttc
      601 gggcgtgcaa aaacgcagcc gcgtcagggc attgacgatc acgcgcagcc ggtccgcgcc
      661 cttgagcgag tcgtcccatt gcgccggctt gttgccgtac atctgcgaca ggaaatcgac
      721 cagatcgggt ccgcgcagcg tcgcctccac ctcggcggcg agcatcagcg tctgcgccac
      781 gtcccattgc ggcagcaccc cgccgtgcac catcagcagg ccgtgggcgc ggatggccat
      841 gcgctgccag cgtagccact ccagcagctc gccgcggtcg cgggccagca gcaccttgtc
      901 catggtgtcg ttgccgtgcg gcgcccggcg cccatgcgcg acggcgagca ggctcaggtc
      961 gtggttgccc agcaggcagc aggccgcgtc gccgaaagcc atgagccggc gcagcaccgc
     1021 gcccgactcc ggcccccgat tgaccaggtc gcccagcatg tacagcgtgt ccctgctggc
     1081 cgagaattcg attttttcga gcaggcggcc cagggcggcg tcgcagccct gtacgtcacc
     1141 gatcaagtaa agtgccatga tgtccgattc tcgctctgat tgatggaagt cctgctgatc
     1201 gcgctgctga cgctgctgaa cggcgtcttt gccatgtcgg agttggcgct ggcctccagc
     1261 cgaaaagccc ggctggtcct gctggcggaa gacggagatg cgggcgccgc ggccgccctc
     1321 aagctgatgg accagcccac ccggtttctg tccacggtgc aggtaggcat cacgtccatc
     1381 ggcgtgctca atggcatcat cggcgaggca gccttcagcg atgggctcgc gaattggctc
     1441 cagatcctgg gcctgggtcc gcgtgcctcg gcttacacag ccaccggaat cgtcgtggcg
     1501 ctgatcacct atataacgat catcttcggc gaactggtac ccaagcggat cggccagctc
     1561 tatcctgaag ccgtgtcgcg cctgctggcg cagccgatga cctggttggc tcgggtggcg
     1621 ggacccttcg tgaagctgct gtccgcgtcg acccagggcg tgctcaagtt gctgcggatc
     1681 gacaccacgc agccgcgcgg catgaccgaa gaggaaatcg cccacagcct ggaagagggc
     1741 gtggacgccg gcgtgatcga gcagcacgag cgccagatgg tggcgaacgt tttccacctg
     1801 gacaaccggc ccctcacgtc catgatggtg ccccgctcgg acgtccaatg gctggatgcc
     1861 agcctgggca tcgcgcaggc gctgcgcgag atcggcgaag gtggcgccca ttcctggtac
     1921 ccggtatgcc gcggctcgct cgacaacgtg ctgggcgtgg tgagcgtggc caggctgctg
     1981 gcgctggggc ccgacccggc gggcaccgtg gagcagcacg cgacgccggc gctgttcgtg
     2041 cccgagacgc tcaccggcat ggagctgctc gaacagttcc gtgcgcggtc cggccgcatg
     2101 gtgttcgtcg tcgatgaatg gggcgtggtg caggggctga tgacgccgca cgacctgctg
     2161 gaagccatca ccggcgagct gcagccggtg gcccaggccg aagcatgggc cacccgccgc
     2221 gaagacggct cctggctgct cgatggacag atgccggtgg ccgaactcaa ggcgcggctg
     2281 gacatccgcg aactgcccga ggaggaacgc ggccgctaca acacgttggc gggcctcttg
     2341 atgtcggtct cgggccgcct gcccgctacc ggagagcgca tcggctgcgg cgactggatg
     2401 ttcgaggtga tcgatctcga cggcaagcgc atcgacaagg tgctggccct gccgctgcaa
     2461 gcggatgcgc agctgcgcgc gcggcgcgga accgccgcct gaacacaggg gaacgatcgc
     2521 gatgtcggat gccgtgtact acgagaagcc ggtgctgctg gaccgcgaca ggcaccgcaa
     2581 gctgcgcgtc ggcgccagcc aggacttcgg tttcgccggc aaggccaatt cgctctacct
     2641 ggcgggcgtg gagttcgccg aggcgtgcaa ggaatatgcc atcgtcttct cgcgcgcggt
     2701 caacggcaag atcgcgcccg tggccatgct cggcttgcgc ggccgcgaaa acctgttcgt
     2761 ggacgacgac gggcgctgga cgggaaccta cgttcccgcg ttcgtgcggc gttatccgtt
     2821 cgtgctggcg gaactgtcgg gcgagagcat gggggtgtgc atcgacgagg ctttcaaggg
     2881 cctgggcgac agccgcggcc aggctctgtt cgacgaccag ggcaacaaca cggttttcct
     2941 gcagcacgcg ctggacttcc tgaaccgctt ccagcaggag taccgccgca ccgaaatctt
     3001 ctgcgaacgg ctggaacgcg cgggtttgct caagcagatg gacgccaggg ccgacctggt
     3061 ggacggccgc accttcaccg tcaggggcct gctggtcgtg gacgagccga agctgatggc
     3121 cttgcccgac gcgacggtgc tgtcgctgtt ccgggcgggc gagctgcacc tggtgtccat
     3181 gcacctggtg tcgctggcca acatgaggca actggtggac cggatggcgc ggcgcgggat
     3241 cccgccgccc gccactacgc ccggctagtg caggccgcgg tgcgggtccg cgccgaccgc
     3301 gaatttggac ccgtcgatca cttcgacggc gccctcttcc accaggcgct gcaagagctt
     3361 gtccacctgc tcagcccgca gtttcgaatg ggcgaggatc cagtgccggt tgatcggccg
     3421 ggtgctcatt accgacagcg cgcgaaagac a
//
LOCUS       DASWIY010000085         4647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2638575, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000085 DASWIY010000000
VERSION     DASWIY010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2638575"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VGA59_03385"
     CDS             complement(<1..186)
                     /locus_tag="VGA59_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9718749.1"
                     /translation="MQVKANGISIEVEDTGSDGAQAGRPAVLLIMGLGMQLVAWPPAF
                     VQALVDQGLRVVRFDNRD"
     gene            complement(195..1049)
                     /locus_tag="VGA59_03390"
     CDS             complement(195..1049)
                     /locus_tag="VGA59_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX9718750.1"
                     /translation="MTQAQQITPELRQWIVEQAQAGHGPETVLKSMLASGWDEDVAVQ
                     ALEATLRAYLEHQAVQQGLPPAVPVPDPNLDESPLYLDAGDRQVCVLQAMYNPRVVVF
                     GGLLSDGECEALIELAKPRLARSLTVETKTGGEEINADRTSNGMFFQRGENELIRRIE
                     ARIARLLNWPEENGEGLQVLHYRPGTQYKPHYDYFDPGEPGTPTILKRGGQRVGTLVM
                     YLAEPDKGGGTTFPDVHLEVAPKRGNAVFFSYERPHPSTRTLHGGAPVLAGEKWIATK
                     WLRERRFL"
     gene            complement(1216..1455)
                     /locus_tag="VGA59_03395"
     CDS             complement(1216..1455)
                     /locus_tag="VGA59_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718751.1"
                     /translation="MAPAVAVALLVALAARIVMPRKSAPRSWWAQALVNSAVGVLVLA
                     AGLWHFGVDGKMATYAALVVAVASCQWVGSRSWRA"
     gene            1555..2814
                     /gene="hemA"
                     /locus_tag="VGA59_03400"
     CDS             1555..2814
                     /gene="hemA"
                     /locus_tag="VGA59_03400"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX9718752.1"
                     /translation="MAVWALGINHTTAPLDLRGRFAYAIDQIEPTLKALRASLSRRPE
                     AAILSTCNRTEIYCAGEHGDMDNTLDWLAHSGGVSSTLLRSHAYTLHDGMAARHAFRV
                     ASGLDSMVLGEPQILGQMKDAVRVADQAGALGTTLNQLFQRSFAVAKEVRSSTEIGAH
                     SISMAAAAVRLAGQLFEDMSKIRVLFVGAGEMVELAATHFAARNPKGIAIANRTLERG
                     ERLASRFGGEVMRLADLPARLHEFDAVISCTASTLPIIGLGAVERALKARRHRPIFMV
                     DLAVPRDIEPEVKDLEDVYLYTVDDLAGVVQTGQANRQAAVAQAEAIIDAGVQSFLHW
                     MDQRRSVPLILQLNAQADEWRAFELARARKMLAKGEDVDAVLEALSKGLTQKMLHGAM
                     AELHAGDAEARQRASSAIQHFFLRKER"
     gene            2920..4038
                     /gene="prfA"
                     /locus_tag="VGA59_03405"
     CDS             2920..4038
                     /gene="prfA"
                     /locus_tag="VGA59_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX9718753.1"
                     /translation="MKTFLRQQLERYPVRLKELDFFLSQPDVVGNMERFRALTREHAE
                     VSEVAGFYERFRQREAALAQAREMLEDPEMAGMAREEIADIEAELPLLEDELQRLLLP
                     KDPDDVRNTFIEIRAGTGGDESALFAGDLLRMYTRYAERQGWRTEIVSVSEGEVGGYK
                     EVVIRIAGEGPGAGARLGPFGKLRFESGGHRVQRVPATETQGRIHTSACTVAALAEPD
                     EAQAVQINPADLRIDTYRASGAGGQHVNKTDSAVRITHLPTGIVAECQDDRSQHRNKA
                     KAMQVLAARIGEKERSERAAREAATRKGLIGSGDRSDRIRTYNFPQGRLTDHRINLTL
                     YKLQFIMEGDLDEVIDALLLARKAEQLEELEFSQGAGR"
     gene            4035..4349
                     /locus_tag="VGA59_03410"
                     /pseudo
     CDS             4035..4349
                     /locus_tag="VGA59_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902236.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
     gene            complement(4534..>4647)
                     /locus_tag="VGA59_03415"
     CDS             complement(4534..>4647)
                     /locus_tag="VGA59_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX9718754.1"
                     /translation="PYIPSLWEDFILRNRRILADQMEFLMAHLPRSSRLHA"
BASE COUNT          788 a         1589 c         1557 g          713 t
ORIGIN      
        1 gtcccggttg tcgaagcgca ccacccgcaa gccctggtcc accagggctt gcacgaacgc
       61 cggcggccag gcgacgagct gcatgcccag gcccatgatc agcaggaccg ccggccggcc
      121 ggcctgtgca ccgtccgagc ctgtgtcctc gacttcgatg ctgatgccgt tggctttgac
      181 ttgcacgccg cccctcacaa gaagcgccgc tcgcgcagcc acttggtggc gatccacttc
      241 tcgcccgcca gcaccggggc gccgccatgc agagtccggg tggacggatg gggacgttcg
      301 tagctgaaaa acacggcgtt gccgcgcttg ggcgccacct ccaggtggac gtcggggaag
      361 gtcgtgccgc cgcccttgtc cggttcggcg aggtacatca ccaaggtgcc cacacgctgg
      421 ccgccgcgct tgaggatggt gggcgtgccc ggctcgccgg ggtcgaaata gtcgtaatgc
      481 ggcttgtatt gggtgcccgg ccggtagtgg agcacctgca atccttcgcc gttttcctcg
      541 ggccagttga gcagccgggc gatgcgcgcc tcgatccggc ggatcagctc gttttcgccg
      601 cgctggaaaa acatgccgtt gctggtgcgg tccgcgttga tctcttcgcc cccggttttc
      661 gtctccacgg tcagcgatcg cgccagccgc ggcttggcca gttcgatcaa tgcctcgcat
      721 tccccgtccg acaacagccc gccgaatacc acgacgcgcg ggttgtacat cgcctgcagc
      781 acgcagacct ggcggtcgcc cgcgtccagg tacagcggcg actcgtccag gttagggtcg
      841 ggcaccggga cggccggagg caggccctgc tgcaccgcct ggtgctccag gtaggcgcgc
      901 agggtcgcct ccagcgcttg cacggccacg tcctcgtccc aaccggaggc cagcatggac
      961 ttgagcacgg tttcgggacc gtggccggcc tgtgcctgct ccacgatcca ttgccgcagt
     1021 tccggagtga tctgctgtgc ctgtgtcatg ttccttcttc ggttccgcgt ctgaacaccc
     1081 tccggtccgg cctcgaagcc cggggatcga tcgagccatc gagcgacttg gcgggcgacg
     1141 gcaggaacag catggcggcc attatgctta gaccggtgcg ctccatggat actcgaggga
     1201 cccggccgcc ccacgtcagg cgcgccagga ccggctgccc acccattggc agctcgccac
     1261 cgccaccacc agggccgcat aagtggccat cttgccatca acgccgaaat gccacagccc
     1321 ggcagccagc accagcacgc cgaccgccga attgacgagc gcctgcgccc accaggaccg
     1381 cggggcggac ttgcgcggca tgacaatccg ggccgccaaa gcgaccagca aggccacggc
     1441 aacggccggt gccatgaagc tcagaaggtg gaggaaaagg tccagggggc ccatcttgag
     1501 ggacttgata aatagcaagt gcttgaaatc ccggcaaatt ttataatgcc gcccatggca
     1561 gtctgggctt tgggcatcaa ccacacgacc gcgccgctcg atttgcgcgg ccgcttcgcc
     1621 tatgccatcg accagatcga gcccacgctg aaggccttgc gcgcgtcact gtcgcgccgc
     1681 cccgaggccg ccatcctgtc gacctgcaac cgcacggaaa tctattgcgc gggcgagcac
     1741 ggcgatatgg acaacaccct ggactggctg gcgcattccg gcggggtctc gtccacgctg
     1801 cttcgctccc atgcctatac cctgcacgat ggaatggccg cgcgccatgc gttccgtgtg
     1861 gccagcgggc tggactcgat ggtgctgggc gaaccccaga tcctcggaca gatgaaggac
     1921 gcggttcgcg tcgccgacca ggccggcgcg ctgggcacga cgctcaacca gctgttccag
     1981 cgttccttcg ccgtggccaa ggaagtgcgc agttccaccg agatcggagc ccattccatc
     2041 agcatggccg ccgcggcggt acgcctggcc ggccagctgt tcgaggacat gagcaagatc
     2101 cgcgtgctgt tcgtcggtgc cggcgaaatg gtcgaactgg ccgccaccca ctttgccgcc
     2161 cgcaacccca agggcatcgc catcgccaac cgcacgctgg aacgcggcga acggctggct
     2221 tcgcgcttcg gcggcgaggt gatgcggctg gcggacttgc cggcgcgcct gcacgaattc
     2281 gatgccgtca tcagctgcac cgccagcacc ctgccgatca tcggcctggg ggcggtggaa
     2341 cgcgcgctca aggcccgcag gcaccggccg attttcatgg tcgacctggc cgtgccgcgt
     2401 gacatcgagc ccgaggtcaa ggacctggag gacgtgtatc tctacacggt ggatgacctg
     2461 gccggcgtgg tccagaccgg ccaggccaac cggcaagccg ccgtggccca ggccgaggcc
     2521 atcatcgacg ccggcgtgca gagcttcctg cactggatgg accagcgccg cagtgtgccg
     2581 ttgatcctgc agctcaacgc gcaggccgac gagtggcgcg ccttcgagct ggcccgggcg
     2641 cgcaagatgc tggccaaggg cgaggacgtc gacgcggtgc tggaggccct gtccaagggg
     2701 ctgacccaga agatgctgca tggcgccatg gccgagctgc atgcgggcga tgccgaggca
     2761 cgccagcgcg ccagctcggc gatccagcat ttcttcctgc gcaaagagcg ttagcggcgc
     2821 gggccgagcc gccgctacgc gtgtcattgc gggcatgacc cgcaatccac ccccgaatgc
     2881 atggatgccg gatccagtcc ggcatgacaa ccgatactca tgaaaacttt cctgcgccaa
     2941 caactcgaac gctatccggt ccgcctcaag gagctggact tcttcctgtc gcagccggac
     3001 gtggtcggca acatggagcg ctttcgggcg ctgacgcgcg agcatgccga ggtcagcgag
     3061 gtggcggggt tctacgagcg cttcaggcag cgggaagccg ccctggcaca ggcgcgggaa
     3121 atgctggaag accccgagat ggccgggatg gcccgggagg aaatcgcgga catcgaggcc
     3181 gaactgcccc tgctggagga cgagctgcaa cgcctgctgc tgcccaagga cccggacgat
     3241 gtgcgcaaca ccttcatcga aatccgggcc ggcacgggcg gggacgaatc ggcgctgttc
     3301 gccggtgact tgttgcgcat gtacacccgc tacgccgagc gccagggctg gcgcaccgag
     3361 atcgtgagcg tgagcgaagg cgaagtgggc ggctacaagg aagtcgtgat ccgcatcgcc
     3421 ggcgagggcc cgggcgctgg cgcccgatta ggccccttcg gcaagctccg cttcgagtcc
     3481 ggcggccatc gcgtgcagcg cgtgccggcg acggaaacgc aggggcgcat ccacaccagt
     3541 gcctgcacgg tggccgcgct ggccgaaccc gatgaggcgc aagcggtcca gatcaatccg
     3601 gcggacctgc gcatcgacac ctatcgcgcc agcggagccg gcggccagca cgtgaacaag
     3661 accgactcgg ccgttcgcat cacccacctg cccaccggca tcgtggccga atgccaggac
     3721 gatcgctcgc agcaccgcaa caaggccaag gccatgcaag tgctggcggc ccgcatcggg
     3781 gaaaaggagc gctccgagcg agccgcgcgg gaggccgcca cccgcaaggg cctgatcggc
     3841 agtggcgacc gcagcgaccg gatccgcacc tacaacttcc cgcaggggcg cctcaccgac
     3901 catcggatca acctgaccct gtacaagctg caattcatca tggaagggga cctcgacgag
     3961 gtgatcgacg cgctgctgct ggctcgcaag gccgagcagc tggaagagct ggaattcagc
     4021 cagggagccg gccggtgacg ctcgccgatg ccctggcggc ggctgccgcc cgcggcctcg
     4081 cgcggctgga cgcgcagttg ctgctgcttc atgcgctggg ccgcccggcc tccgaccgcg
     4141 cctggttgct ggcccacgac acggacaccc ttgccgaccc ggcacgtgcg gcttacctgg
     4201 cgctgtgcga gcgccgggcc ggcgccgagc cgctggctta catcgtcggc cacaaggagt
     4261 tcttcgggat ggacctgtgc gtcgacaaac gggtcctggt gccgcggccg gagaccgaaa
     4321 cgatcgtcga gtgggcgctt gaaatccttg gctgtcatcc cggccccgga tcaagtccgg
     4381 ggtttcagcg cccgggatcc aggctgggga ttgcggggcc ggaccggagc gtgatgcctc
     4441 ggcacgtgca tagcgtaggc ccggcattgg gctcgctgca ctggacagct gcgcgacccg
     4501 ggcgttccgc gagcccggcc cgcaatgaca acgctacgcg tgcagccgcg acgacctggg
     4561 caggtgcgcc atcaggaact ccatctggtc ggccaggatg cggcggttgc gcaggatgaa
     4621 gtcttcccac aggctgggga tgtaggg
//
LOCUS       DASWIY010000086         9016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1163175, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000086 DASWIY010000000
VERSION     DASWIY010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9016
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163175"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1114)
                     /locus_tag="VGA59_03420"
     CDS             complement(236..1114)
                     /locus_tag="VGA59_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9718755.1"
                     /translation="MKPLGRLWVCVALLCLPWAGAAHAADYSGPLFDAHLHYNDEAQS
                     PHPLSDVLARMQRNGVKAIIANSRPNDGTKALALAPQTRDAGVTVVPFVRLYRNRADY
                     DNWFRDESIYEMVQGELARGTAAGPFRGLGEFHLYDSTNANGPVAQKLMALAEEKQLA
                     VLAHVDDAAIDLLMANTPSKGRNVRLIWAHTGIGGTPPARVDQLLARYPLLMGELSYR
                     PGLTCEGGKLCPEWRELLLKYPTRFMIGSDTWVNQRWLYYDELMNAYRAWLGDLPAES
                     ARRIAWDNGAGLFGFK"
     gene            complement(1347..2030)
                     /locus_tag="VGA59_03425"
     CDS             complement(1347..2030)
                     /locus_tag="VGA59_03425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9718756.1"
                     /translation="MGNFVDLKAADGFAFPAYVAQPAGKARAGIVVLQEIFGVNSHIR
                     SVADGYAAEGYLAVAPSTFHRVKPGVDIGYSPDDISAGSALKAAVELLPAPGVMQDIE
                     AAIGHAAAAGKVGIVGYCWGGLLTWRAACMLDGLAAAVPYYGGGMTTPDEVVRAPKAP
                     VLAHFGDKDRWISPTSVEAFKKAHPEVEVRIYHADHGFNCDQRASYDPAAAKQARELT
                     LAFFAKHLR"
     gene            2184..2930
                     /locus_tag="VGA59_03430"
     CDS             2184..2930
                     /locus_tag="VGA59_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9718757.1"
                     /translation="MGQVLLVTGGGRGIGAATALLGARQGFAVAVNYTANAPAADDVV
                     RQIRAGGGTAFSVQADVAREDRVMAMFEKVDAELGRITALVNNAGVVDATARVDEMSF
                     ARLRRMFDVNVIGSFLCAREAVRRMSTRHGGNGGAIVNVSSAAARLGSPGQYVDYAAA
                     KGAIDAFTLGLAKEVAAEGIRVNAVRPGLIDTGIHASGGLPDRVRQLAHEVPMQRGGT
                     PAEVAEAIVWLLSPAASYTTMSLLDVSGGR"
     gene            2930..3418
                     /locus_tag="VGA59_03435"
     CDS             2930..3418
                     /locus_tag="VGA59_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9718758.1"
                     /translation="MSLIKYYWEDLQTGSVRDLGTVTLSAEEIKDFAVQFDPQPFHMD
                     EAAGRRSIFGNLCASGWHTCALAMKLTVENFLHEAASMGSPGVENLRWLKPVYPGDTL
                     RLQQTIVESRALRTRADTGLVRSVWDMFNQNGEKVMRMEGYGMFRRRHPATPEEIAGL
                     EA"
     gene            3422..4054
                     /locus_tag="VGA59_03440"
     CDS             3422..4054
                     /locus_tag="VGA59_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX9718759.1"
                     /translation="MDFKPLVTLLAVVNPLAIVPFFIHYTQGFSREQRRHTVVTAAFS
                     AFLVIAASALLGLRILEFFGISLASFQVGGGMLLLISAMNMLNAQPAEAKPLTNDLAD
                     GAQKAAMGASIAVVPLTIPLLTGPATISTVVIYAEQAQSLLQISALIGYGVVIGVATA
                     VCFALAEPIARFLGKTGINVMTRLMGLILAALAVEVMALGLLKLFPALGH"
     gene            complement(4098..4649)
                     /locus_tag="VGA59_03445"
     CDS             complement(4098..4649)
                     /locus_tag="VGA59_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9718760.1"
                     /translation="MAKQVVEEQLQSRGLELVGPCERLSLRSELLELSALLRDFTPAE
                     ADLLGASMVLVRAQPGQLLIAEGEASDWMMLLLRGTVDVGKRKIGAAVDEQEPGDIAR
                     LAVLKEGAVVGEMSMLDGEPRYASCWALSEVEAAVLSRYAVARLISAHPAVGAKLLVK
                     LTQLLAQRLRNTSSQLVKALQKE"
     gene            complement(4659..5303)
                     /locus_tag="VGA59_03450"
     CDS             complement(4659..5303)
                     /locus_tag="VGA59_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX9718761.1"
                     /translation="MTRPLAAAFNAMLLVAGLAGCAGLVPDKPARATLYDFGPGATAM
                     PAAGRQPSQPSLVLADIGASGALDGSAVLYRLGYADPNQLRPYAHARWSAPPPQLIRQ
                     RLRQQLARERVVLDPGESAALARNGGVAPRVLRLDLEEFSQLFESQAESWGLLRLRAT
                     LLDNTPAGEKLLAQRSFVVRQAAPTPDAPGGVRALGAATDAAAEEIAQWLAQLR"
     gene            complement(5300..6259)
                     /locus_tag="VGA59_03455"
     CDS             complement(5300..6259)
                     /locus_tag="VGA59_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9718762.1"
                     /translation="MENKAHAIAAGIFVVAVTVLLVALAAWLTRDTGARDVYEISTRE
                     TVTGLQPQAPVRYRGVDVGKVASIGFDPKATGNVLLRLEIDREAPVTSDTFATLSFQG
                     ITGLAFIQLDDTGKPAPRLVGDNGDNPPRIPLKPGLLTKLADKGEVILDQVEQVATRF
                     NRLLGDENQRRISNALANLDTAATDASRLAQRLDSTVAKRVDPALAEASTTLRAVRKS
                     ADAVEKTAADFSDTARRLNDKDGPMDRLAEGSQALSHAADSFNAATLPRINRVTEDTS
                     RAVRLLSRTVTTINDNPQSLIFGAGPVAPGPGEPGFTPPGAAK"
     gene            complement(6272..7048)
                     /locus_tag="VGA59_03460"
     CDS             complement(6272..7048)
                     /locus_tag="VGA59_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9718763.1"
                     /translation="MVEIRKLWTVFTTPEKRFVVHQDLDLTVQRGEVLSLVGGSGSGK
                     TVLLRQILGLETPTQGEVYVLGQPVGQMGRKGAASRIGMLFQHGALFSAFSVLENIAF
                     PLRELKTLPAALVREAAMVKLQMVGLAPEDAAKMPADLSGGMIKRAALARALVMDPPL
                     LLLDEPTAGLDPDSSDAFVSLLRSLHRDLELTVIMVTHDLDTLFELSTRIAVLADKRV
                     IVAATPKEVIAFQHPFIHDFFLGERGQRAMELLREYPFAV"
     gene            complement(7095..8213)
                     /locus_tag="VGA59_03465"
     CDS             complement(7095..8213)
                     /locus_tag="VGA59_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9718764.1"
                     /translation="MPETTPRIEQQPAPEGARLRLLGQWTAAQFAQPRLLKSLRASLE
                     ATTVQQAWDLRQAERLDHVGAQLLWDRWGREWPRQLELLPAQRAVLERVSRFAVTPPP
                     RKKATWMGSYLALGEAVLRAGDHVKSFIGLIGQLTLNLLRLARAPQEGPWRDFSGHLY
                     RIGATALPITALVGFLIGVVLAYLTSQQLKQFGADAYIVNILGIALIRELGPMLAAIL
                     IAGRSGSAITAQIGVMRVTEELDAMRVMGISHSFRLVMPRAMALAVAMPLISVWTTLC
                     ALAGGMLAADVSLGITPTFFMTALPEAVSLSTLILASAKSVVFGVLIALIACHFGLRV
                     QPNTQSLGEGTTASVVASITAVILVDALFAVLFKDVGL"
     gene            8471..>9016
                     /locus_tag="VGA59_03470"
     CDS             8471..>9016
                     /locus_tag="VGA59_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9718765.1"
                     /translation="MSERATIPELLVEQRGPALWLTIQREERRNALSHEVLSQMTRAI
                     DAAQADRALRAIVVTGAGTRAFCAGADLQSAQAFTTDYSEPYASLALLLRRAKASNLP
                     LIARVNGACMAGGMGLLAMCDMAVAAGHAVFGLPEVKVGVFPAQVLSVLQHLIPRRKL
                     AELCLTGEPLTAAQALEYGLVN"
BASE COUNT         1413 a         3109 c         3066 g         1428 t
ORIGIN      
        1 atacagttat ccgctgctga tggccgtcac catcgcgggc tgtgtcctcc tgtaccgcaa
       61 gctcaggcgc gcggggtggg tatgacggaa agccgccacc cggccggacg ggagaatgaa
      121 acggctcacc gagccggcga actccggcaa gcctcgacag gagaagaagc cctagacgtc
      181 agggtcgccc gtcttgagcg ggggggccgg tttccgacaa ggttgtccgc ccgcttcact
      241 tgaagccgaa caggccggcg ccgttgtccc acgcgatccg gcgcgcgctc tcggccggca
      301 ggtcgccgag ccaggcgcgg taggcgttca tcagctcgtc gtagtacagc cagcgctggt
      361 tgacccaggt gtccgatccg atcatgaagc gggtcgggta tttcagcagc agttcgcgcc
      421 actcggggca gagcttgccg ccctcgcagg tcagccccgg ccggtacgac aactcgccca
      481 tcagcagcgg atacctggcc agcagctggt cgacccgcgc cggcggcgtg ccgccgatcc
      541 cggtatgggc ccagatcagg cgcacgttcc ggcccttgga tggcgtgttc gccatgagca
      601 ggtcgatcgc cgcgtcgtcg acgtgcgcca gcaccgccag ctgtttctcc tcggccagcg
      661 ccatcagttt ttgcgcgacc ggcccattgg cattggtgct gtcgtacagg tggaactcgc
      721 ccaggccccg gaacggcccc gcggcggtgc cccgcgcgag ttcgccctgc accatctcgt
      781 aaatcgattc gtcgcggaac cagttgtcgt aatcggcgcg gttgcgatac aggcgcacga
      841 acggcaccac cgtcacgccg gcatcccggg tttgcggcgc caaggccagg gccttggtgc
      901 cgtcgttggg gcgcgagttg gcgatgatgg ccttgacgcc gttgcgctgc atccgtgcca
      961 gcacgtcgga cagcggatgc ggcgattgcg cttcatcgtt gtagtgcagg tgcgcgtcga
     1021 acagcggccc gctgtagtcg gccgcgtggg ccgctccggc ccatggaagg cacaacagcg
     1081 cgacgcacac ccagagcctc cccagcggct tcatggaaat tcgcgccgcg cacagcgtac
     1141 gcaatcgcgc ggccagtgcg ggggccaccg cggatccggc tttgccggtc cgctggtggg
     1201 ccttgcaggc cttatgaacc cggcgcggcc gagcccaggg ccaccgcgcg gatccggctt
     1261 tgccggtccg ctggtggcgc ccccttgagg gggagagggc gaagcccgag ggggcatcgg
     1321 ccgcaggccg cttcgggggt gggctatcac ctcaggtgct tggcgaagaa tgccagggtc
     1381 agctcgcgtg cctgcttcgc cgctgccggg tcgtacgatg cgcgctggtc gcagttgaag
     1441 ccgtggtcgg cgtggtaaat gcggacctcc acttcgggat gggctttctt gaacgcttcc
     1501 acgctggtcg gcgagatcca gcggtccttg tccccgaaat gggccagcac aggcgccttg
     1561 ggtgcgcgca cgacctcgtc cggcgtcgtc atcccgccgc cgtagtacgg caccgcggcc
     1621 gccaatccat cgagcatgca ggccgcgcgc cacgtgagca ggccgcccca gcaatagccg
     1681 acgatgccga ccttgcccgc tgccgcggcg tgaccgatgg ccgcttcgat gtcctgcatg
     1741 acgccgggcg cgggcagcaa ttcgaccgcg gccttgagtg cggagccggc gctgatgtcg
     1801 tcgggcgagt agccgatatc cacccccggc ttgacccggt ggaacgtgga cggcgcgacg
     1861 gccaggtaac cttcggccgc gtagccatcg gccaccgatc ggatatgcga attgacgccg
     1921 aagatttcct gcagcaccac gatgcccgcc ctggcttttc cggccggctg cgccacgtag
     1981 gcgggaaagg caaagccgtc ggcggccttg agatcaacga aattgcccat ctgcatctcc
     2041 ttgaattgtc gctgttcgaa gcccgtaccg gatcgtcggg catagtccag aagacccggc
     2101 gcagcctgcg gctcatcttt gctgtaatgg cggtcggcga gcatgcaagc ttaccgccgg
     2161 atttttgtgc gaggagacgg attttgggac aggtactact ggttaccgga ggcggccgcg
     2221 gcatcggggc cgcgactgcg ctgctgggcg cccggcaggg ctttgccgtc gcggtcaact
     2281 acaccgccaa tgcgccggcg gccgacgatg tggtgcgcca gatccgcgcg ggcggcggca
     2341 cggccttctc ggtgcaggcg gacgtggcgc gggaagaccg cgtcatggcg atgttcgaga
     2401 aggtcgacgc cgagctcggg cggatcacgg cactcgtcaa taacgccggg gtcgtggacg
     2461 cgacggcccg agtggacgag atgagcttcg cgcgcctgcg gcgcatgttc gacgtcaacg
     2521 tgatcggctc gttcctgtgc gcccgcgagg cggtccgacg catgagcacg cgccacggcg
     2581 gcaatggcgg cgccatcgtc aacgtgtcca gcgccgccgc gcggctgggc tcgcccggcc
     2641 agtacgtgga ctatgccgcg gccaagggcg cgatcgacgc cttcaccctc ggcctggcga
     2701 aggaagtggc ggccgaggga atccgcgtca acgccgtgcg gccgggtctg atcgacaccg
     2761 gcatccatgc ctccggcggc ttgcccgacc gcgtgcggca actggcccac gaggttccga
     2821 tgcagcgcgg cggcacgccc gcggaagtgg ccgaggccat cgtctggctg ctctcgcccg
     2881 cggcaagtta caccaccatg tcgctgctcg acgtctccgg aggccgctga tgagcttgat
     2941 caagtattac tgggaggacc tgcagaccgg ctcggtgcgc gatctcggga cggtgacgct
     3001 gtcggccgag gagatcaagg atttcgccgt gcagttcgat ccgcagccct tccatatgga
     3061 cgaggcagcc gggcgccgtt cgatcttcgg caatctctgc gccagcggct ggcatacctg
     3121 cgccctggcg atgaagctca cggtggagaa tttcctgcac gaagcggcca gcatggggtc
     3181 tccgggcgtg gagaacctgc gctggctcaa gcccgtctat ccgggcgaca cgcttcgcct
     3241 gcaacaaacc atcgtcgaat cgcgcgcgct gcgaacgcgc gccgataccg ggctggtccg
     3301 ctcggtctgg gatatgttca accagaacgg cgaaaaagtg atgcggatgg agggctatgg
     3361 catgttccgc cgccgccatc cggccacgcc ggaagaaatc gcgggcctcg aggcctaacg
     3421 catggacttc aagcccctgg tgaccttgct cgcggtggtg aatccgctgg ccatcgtgcc
     3481 gttcttcatc cactacacgc agggattttc gcgggagcag cggcgccaca cggtcgtcac
     3541 ggcggccttc agcgccttcc tggtgatcgc cgccagcgcg ctgctgggcc tcaggatcct
     3601 ggagttcttc ggcatctcgc tggcgagctt tcaggtcggc ggcggcatgc tgctgctgat
     3661 cagcgccatg aacatgctca acgcgcagcc cgccgaggcc aagccgctca cgaacgacct
     3721 ggccgacggc gcgcaaaaag ccgcgatggg cgccagcatc gcggtcgttc cgctgacgat
     3781 tccgctgctc accgggccgg cgacgatctc cacggtggtg atctacgccg agcaggcgca
     3841 gagcttgctg cagatttcgg cgctgatcgg ctacggcgtg gtgatcggcg tggccacggc
     3901 cgtgtgcttc gccctggccg aaccgatcgc ccgcttcctg ggcaagacgg gcatcaatgt
     3961 gatgacgcgg ctgatgggcc tgatcctggc ggcgctggcg gtggaagtga tggccctcgg
     4021 actgctcaag ctgtttcccg cgctgggaca ttaaggagcc ttcagaggac agggtcttca
     4081 ggcctgtcct cgcgcactca ttctttttgc agcgccttga ccagctggct gctggtgttg
     4141 cgcaggcgct gtgccagcag ctgcgtgagc ttgacgagca gcttggcgcc cacggcgggg
     4201 tgcgccgaga tcaggcgcgc cacggcgtac cggctgagca cggcggcctc gacttcgctc
     4261 agggcccagc aactcgcgta gcgcggctcg ccgtcgagca tggacatttc gcccaccacc
     4321 gcgccttcct tgagcaccgc cagccgcgcg atgtcgcccg gctcctgctc gtccacggcg
     4381 gcgccgatct tgcgcttgcc cacgtccacg gtcccgcgca gcaacaacat catccagtcg
     4441 ctggcctcgc cctcggcgat cagcagctgg ccgggctgcg cccgcaccag caccatgctg
     4501 gctcccagaa ggtccgcctc ggccggggtg aagtcccgca gcagtgccga aagctccagc
     4561 agctccgatc gcagcgacag ccgctcacaa ggcccgacca gctccagacc ccgggattgc
     4621 agctgctctt cgactacctg tttcgccatg acggtcccct atcgcagctg ggccagccac
     4681 tgcgcgatct cctcggccgc ggcgtcggtg gccgcaccca gcgcacgcac tccgcccggc
     4741 gcatccggcg tgggcgcggc ctgacgcacg acgaaactgc gctgggccag cagtttttcg
     4801 ccggccggcg tgttgtccag cagcgtcgcg cgcagccgca gcaagcccca gctctcggcc
     4861 tgcgattcga acagctgcga gaactcttcc aggtcgaggc gcagcacacg cggcgccacg
     4921 cccccgttgc gcgccagggc cgcgctctcg ccggggtcga gcacgacgcg ctcgcgcgcg
     4981 agttgctggc gaagccgctg gcggatcagc tgcggcggcg gcgcgctcca gcgggcgtgg
     5041 gcgtagggcc gaagctggtt cgggtcggcg tagcccaggc gatagagcac ggccgaaccg
     5101 tccagagcgc cggaagcgcc gatgtcggcc agcaccagcg aaggctggct cggctgccgg
     5161 cccgcggccg gcatcgccgt cgcgcccggc ccgaagtcgt acagggtggc acgcgccggc
     5221 ttgtcgggca caaggccggc gcagcctgcc agccccgcca cgagcagcat cgcattgaac
     5281 gcggcagcca gcggtcgggt catttggccg ctcccggggg cgtgaagccc ggctcgcccg
     5341 gcccgggcgc caccggcccg gcgccaaaga tcagcgattg cgggttgtcg ttgatcgtcg
     5401 tcacggtgcg actgagcagg cgcacggcgc gcgacgtgtc ctcggtgacg cggttgatgc
     5461 gcggcagggt cgcggcgttg aacgagtccg cggcgtgcga cagcgcctgc gagccttcgg
     5521 ccagccggtc catcgggccg tccttgtcat tcaggcggcg tgccgtgtcg ctgaagtccg
     5581 cggcggtctt ttcgacggcg tccgcgctct tgcgcaccgc gcgcagcgtc gtcgacgctt
     5641 cggccagcgc gggatcgacc cgcttggcga ccgtcgaatc gaggcgctgt gccagacggc
     5701 tcgcatcggt ggccgcggtg tcgagatttg ccagcgcgtt gctgatgcgg cgctggttct
     5761 cgtcgccgag aagccggttg aagcgcgtcg ccacctgctc cacctggtcc aggatcacct
     5821 cgcccttgtc cgcgagtttg gtcagcaggc cgggcttgag cggaatacgc ggcgggttgt
     5881 cgccgttgtc accgaccagg cggggcgccg gctttccggt gtcgtcgagt tgaataaacg
     5941 ccaggcccgt gatgccctgg aaactgagcg tcgcgaaggt gtcgctggtc accggcgcct
     6001 cccggtcgat ctccaggcgc agcagcacgt tcccggtcgc cttgggatcg aagccgatcg
     6061 aggcaacctt gcccacgtcg acgccgcgat agcgcaccgg cgcctgcggc tgcagccccg
     6121 tgaccgtttc gcgcgtggaa atctcataga cgtcgcgcgc gcccgtgtcg cgcgtgagcc
     6181 aggccgccag cgcgaccagc agcacggtga ccgccacgac gaagatgccg gcggctatcg
     6241 catgagcctt gttttccatg gagcgtcctt tctatacggc aaacggatac tcgcgcaaca
     6301 gttccatcgc gcgctggccc cgctcgccga gaaaaaaatc gtggatgaat gggtgctgga
     6361 acgcgatcac ttccttgggc gttgccgcca cgatcacccg cttgtccgcc agcaccgcga
     6421 tgcgcgtgga cagctcgaac agcgtgtcca ggtcgtgcgt gaccatgatc accgtcagct
     6481 ccaggtcgcg gtgcagcgaa cgcagcagcg acacgaaagc atccgagctg tcggggtcca
     6541 gccccgcggt cggctcatcc agcagcagca gcggcgggtc catgaccagg gcgcgggcca
     6601 gtgccgcgcg cttgatcatg ccgcccgaca ggtcggccgg catcttggcg gcgtcctcgg
     6661 gcgccaggcc gaccatttgc agcttcacca tcgccgcctc gcgcaccagt gcggccggca
     6721 aggtcttgag ttcgcgcagc gggaatgcga tgttttccag cacgctgaag gcggagaaca
     6781 gcgcgccatg ctggaacagc atgccgatgc ggctggccgc gcccttgcgc cccatctgtc
     6841 ccaccggctg tcccagcacg tacacctcgc cctgggtcgg ggtttccagg cccaggatct
     6901 ggcgcaacag caccgtcttg ccactgccgg agccgcccac cagcgacagc acctcgccgc
     6961 gctgcaccgt caggtccagg tcctggtgga ccacgaagcg tttctcgggc gtggtgaata
     7021 ccgtccacag cttgcggatc tccaccatcg gcgcaccgcc ggcgggcagg gggatgtcgt
     7081 tcaggtccac ggcttcaaag ccccacgtcc ttgaacagca cggcgaacag cgcatcgacc
     7141 aggatcaccg ccgtgatcga ggcaaccacc gaggccgtcg tgccttcgcc caggctctgg
     7201 gtgttgggct gcacgcgcaa gccgaagtgg caggcgatca gggcgatcaa caccccgaac
     7261 accaccgact tggcgctggc gaggatcagg gtgctcaggc tcacggcctc gggcagggcc
     7321 gtcatgaaaa aggtcggcgt gatgcccagg gacacgtcgg ccgccagcat gcccccggcc
     7381 agcgcgcaca aggtggtcca cacgctgatc agcggcatgg ccacggcgag cgccatcgcg
     7441 cgcggcatca cgaggcggaa actgtgggaa atacccatca cccgcatggc gtcgagttcc
     7501 tcggtgaccc gcatcacgcc gatctgcgcc gtgatcgccg agcccgaacg gccggcgatc
     7561 aggatcgccg ccagcatggg cccgagctcg cgtatcagcg cgatgcccag aatgttgacg
     7621 atataggcgt ccgcgccgaa ctgcttgagc tgctgcgagg tcaggtaggc cagcaccacc
     7681 ccgatcagga accccaccag ggccgtgatc ggcaaggcag tggcgccaat gcggtaaagg
     7741 tggccggaga aatcccgcca ggggccttcc tgcggcgcgc gcgccagccg cagcaggttc
     7801 agcgtgagct gcccgatcag gccgatgaac gatttcacgt gatcgccggc acgcagcacg
     7861 gcctcgccca gcgcgaggta ggaccccatc caggtcgcct tcttgcgcgg cgggggcgtc
     7921 accgcaaaac gggagacgcg ctccagcacc gcccgctgcg cgggcagcaa ttccagctgc
     7981 cgcggccatt cgcggcccca gcggtcccac agcagctggg cgcccacgtg gtccaaccgc
     8041 tcggcctggc gcaagtccca ggcctgttgc accgtggtgg cctccaggct ggcccgcagc
     8101 gacttcagga gccggggctg cgcgaactgc gccgccgtcc actggcccag cagccgcagg
     8161 cgagcccctt ccggcgcagg ctgctgctcg atgcgaggcg tggtttccgg catgtaagcg
     8221 gttgtcccag gagctgagcc tgccatcgta actgcacggc cgtgcaggcg ctgtaggaat
     8281 cagcctgcgc gggggcgcgg cagcgacaag gcgacagggt cgccgcgggt ataaagtgcc
     8341 gaacgacaag gagacaagtc atggagcgaa tggcgagggg ccccagcttg ctggggatcg
     8401 acacgcgtaa ggacttgggt gtggcgactc ccccgcctta ccacaacctc acgacagctc
     8461 ggagccacac atgagcgagc gcgccacgat ccccgaattg ctggtggaac agcgcggccc
     8521 ggcgctgtgg ctcaccatcc agcgcgaaga gcgccgcaac gcgctcagcc acgaggtcct
     8581 ttcgcagatg acccgggcca tcgacgccgc ccaggccgat cgtgcgctgc gcgcgattgt
     8641 ggtgaccggc gccggcaccc gggccttctg cgccggggcc gacctgcaat cggcgcaggc
     8701 tttcaccacc gattactccg agccttacgc ttccctggcg ctgctgctgc ggcgggccaa
     8761 ggccagcaac ctgccgctca tcgcgcgcgt caacggcgcc tgcatggccg gcggcatggg
     8821 cctgctggcg atgtgcgata tggccgtggc agccggccat gcggtgttcg gcctgcccga
     8881 ggtcaaggtc ggggtgttcc cggcccaggt cctgtcggtg ctgcagcacc tgattccgcg
     8941 gcgcaagctc gccgagctat gcctgaccgg cgagccgctg accgcggccc aagccctcga
     9001 gtacggcctg gtcaac
//
LOCUS       DASWIY010000087         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_55755, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000087 DASWIY010000000
VERSION     DASWIY010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55755"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VGA59_03475"
     CDS             complement(<1..862)
                     /locus_tag="VGA59_03475"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="catalyzes the transfer of a segment of a
                     1,4-alpha-D-glucan chain to a primary hydroxy group in a
                     similar glucan chain; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEX9718766.1"
                     /translation="MNSSSLLSDTLIDALLSARLHDPFRLLGVHHTPEGWRLRVFQPA
                     ADAVWLRLPTGVAPLERVHRAGLFEWCGTQPPPAPHVLGVSEGGKVRETHDPYAFAPV
                     LSGHDLYLFNEGRLRQAWRALGSHVEERSGVTGTRFAVWAPNAERVSVVGDFNRWDGR
                     MHPMAVHGSSGVWELFIPDLRGGELYKYEIRNRATGEVMLKTDPYAREFERRPATAAR
                     VRAEGAYPWSDAGWMEHRRNWDWQHAPMSIYEVHLGSWRRAPESGDRWLSYREIAPLL
                     AEHVAALGFTH"
     gene            1085..>2046
                     /gene="glgC"
                     /locus_tag="VGA59_03480"
     CDS             1085..>2046
                     /gene="glgC"
                     /locus_tag="VGA59_03480"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX9718767.1"
                     /translation="MGQDSSSNLARGPAGPDVRFISQLTSSTYGLILAGGRGSRLMQL
                     TDWRAKPAVPFGGKFRIIDFPLSNCVNSGIRRIGVATQYKSHSLIRHLQRGWSFLDGR
                     LKEFIDLLPASQRMQEDTWYQGTADAVYQNLDILKTNNPEYILILAGDHVYKMDYGRM
                     LAYHVSRQADVTVGCIDVPVEDARAFGVMGVDDDLRVRSFLEKPPSPPSIPGQPGRAL
                     ASMGIYVFNAKFLYEQLARDADEPKSNHDFGKDIIPHLVPRYRVYAHSFFDSCVGMND
                     NRAYWRDVGTVDAYWEANVDLIHVTPELNLYDKVWPIWTYQEQLP"
BASE COUNT          339 a          710 c          680 g          317 t
ORIGIN      
        1 cgtgcgtgaa cccgagcgcc gcgacgtgct ccgccagcag cggcgcgatc tcgcggtagc
       61 tcagccagcg gtcgccactc tcgggcgcgc gccgccacga tccgaggtgc acctcgtaga
      121 tgctcatcgg tgcatgctgc cagtcccagt tgcgacgatg ctccatccag cccgcgtcgc
      181 tccacgggta tgcgccctcc gcccgcacgc gcgccgccgt ggcgggcctg cgctcgaact
      241 cgcgcgcata ggggtcggtc ttcaacatca cttcgccggt tgcgcggttg cggatctcgt
      301 acttgtacag ttccccgccg cgcaggtcgg ggatgaacag ctcccagacg ccgctggagc
      361 cgtgcaccgc catgggatgc atgcgtccgt cccagcggtt gaagtcgccg accacgctga
      421 cccgctccgc gttcggcgcc cagacggcaa agcgcgtgcc cgtaacgccg ctgcgctcct
      481 ccacgtggct gcccagggcg cgccaggcct gccgcagccg cccctcgttg aacaggtaca
      541 gatcatgccc gctcagcacc ggcgcgaaag cataggggtc gtgggtttcg cgcaccttgc
      601 cgccctcgct gacgcccagg acgtggggcg ccggcggggg ttgtgtaccg caccactcga
      661 acaggccggc ccggtggacg cgctccagcg gcgcgacgcc ggtcggcaac cgcagccaca
      721 ccgcatcggc ggccggctgg aacacgcgca ggcgccagcc ctcgggggtg tgatgcaccc
      781 cgagcagccg gaaagggtcg tgcagacggg ccgacagcag ggcgtcgatg agcgtgtcgg
      841 agagcaggga agaggagttc atgccagggt gggtggtgcg ggccgctgat gttggttgtt
      901 tgggggctgg gtagccgggg ccggacaccg cacgtattat aggcggcggg tgcccggttt
      961 cccagatcta cctggctgtt ccgggcgaga tcgaccgcag ctggacctgc gccggagcgc
     1021 cctcggatga acagccgctg gccgccgcga cacgaacgga ccgcatcgag caaggacgac
     1081 gaccatggga caggattctt cgagcaacct agcgcgtggg ccggccggcc cggacgtgcg
     1141 cttcatcagc cagctgacca gcagcaccta cgggctgatt ctggcggggg gtcgcggcag
     1201 ccggctgatg cagctcaccg actggcgcgc gaagccggcg gtgcccttcg gcggcaagtt
     1261 ccgcatcatc gacttccctt tgtccaactg tgtgaactcc ggcatccgcc gcatcggcgt
     1321 ggccacccag tacaaatcgc acagcctcat ccgccatctg cagcgcggct ggagcttcct
     1381 ggacgggcgc ctgaaggagt tcatcgacct gctgccggcc tcgcaacgca tgcaggaaga
     1441 cacctggtat cagggcaccg ccgatgcggt gtatcagaac ctggacatcc tcaagaccaa
     1501 caacccggag tacatcctga tcctggccgg cgaccacgtc tacaagatgg actacgggcg
     1561 catgctcgcc taccacgttt cccggcaggc ggacgtcacc gtgggatgca tcgacgtgcc
     1621 ggtggaggat gcgcgcgcct tcggcgtcat gggcgtggac gatgatctgc gcgtgcgcag
     1681 ctttctggag aagccgccga gcccgccttc cattccgggc cagcccggcc gggcgctcgc
     1741 cagcatgggc atctacgtgt tcaacgccaa gttcctctac gagcagctgg cgcgcgacgc
     1801 cgacgagccc aagtccaacc acgacttcgg caaggacatc atcccgcacc tggtgccgcg
     1861 ttatcgcgtg tacgcgcaca gcttcttcga cagctgcgtc gggatgaacg acaaccgcgc
     1921 ctactggcgc gacgtgggca cggtggacgc ctactgggag gcgaacgtgg atctcatcca
     1981 cgtcaccccc gagctcaacc tgtacgacaa ggtgtggccg atctggacct accaggagca
     2041 gctgcc
//
LOCUS       DASWIY010000088         6353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_56989, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000088 DASWIY010000000
VERSION     DASWIY010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56989"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /gene="ccoO"
                     /locus_tag="VGA59_03485"
     CDS             <1..600
                     /gene="ccoO"
                     /locus_tag="VGA59_03485"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEX9718768.1"
                     /translation="FSHEKIETSNFLMIVLILLVVAVGGLVEIVPLFFQKTTTQAIEG
                     VKPYTALQLAGRDVYLREGCYNCHSQMIRPFRAETMRYGHYSVAGEFVYDHPFQWGSK
                     RTGPDLHRVGGKYSDEWHRIHLNNPRDVVPESNMPAYPWLEKGVVDAAGMAGRMKALR
                     MVGVPYSDEEIAKAAADVKDKTDMDALVTYLQAMGRALK"
     gene            864..1001
                     /locus_tag="VGA59_03490"
     CDS             864..1001
                     /locus_tag="VGA59_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEX9718769.1"
                     /translation="MDITTLRIIATLASFGTFLGIAWWAYARHNRDRFDEAARIPFEQ
                     D"
     gene            1015..1932
                     /gene="ccoP"
                     /locus_tag="VGA59_03495"
     CDS             1015..1932
                     /gene="ccoP"
                     /locus_tag="VGA59_03495"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEX9718770.1"
                     /translation="MSDFTANFWSVYVAGFTVVSIIACLLLLWITARSKVVPNADNTT
                     GHVWDGDLRESNNPMPRWWMWLFVITIVFGLLYLVAYPGLGSYPGELKWTTSGEYAAE
                     VEQANKELAPMYAQFTAKKAEELAGDANAMAVGERLFMNNCAQCHGSDARGSKGFPSL
                     ADADWLHGGATGKIAETITQGRRGQMPPMAAAVGTPEDVKNVANYVLSLSRSPHDSVR
                     AQLGKAKFTACAACHGMDGKGNPALGAPNLADDIWLHGWGEQAIVAMVNTGKINEMPA
                     QAGKLTEAQIHVLAAYVWGLSNKPAPGKL"
     gene            1943..3376
                     /gene="ccoG"
                     /locus_tag="VGA59_03500"
     CDS             1943..3376
                     /gene="ccoG"
                     /locus_tag="VGA59_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEX9718771.1"
                     /translation="MQSTAARKVIPIAAAAAEAEEGESLYEAQKKIYPRAVRGLFARW
                     RWGLVFLTQLVFYGLPWLHWGERQAVLFDLAARRFYLLGYVLYPQDFIYLTGLLVICA
                     LSLFLFTAVAGRLWCGFACPQTVYTEIFLWIERKLEGDRNARMRLDAAPMSARKFLKK
                     GGKHFLWMALAIWTGFTFVGYFTPIRTLWAQSFTLSFGPWEWFWVNFYGLATYGNAGF
                     LREQVCKYMCPYARFQSAMFDQDTLIVSYDTGRGEPRGSRSRKADPAALSLGACVDCS
                     LCVHVCPTGIDIRQGLQYECIGCAACVDVCDGVMDKMNYPRGLIRFTTQNGMAHGWSR
                     SQIWRHVLRPRVLVYGAVLVALSAALLGSLVVRTPLKVDVVRDRAALSRIVAGGKLEN
                     VYRLQIMNATEQPQRYRISAEGLEGLALASDAVVAVGPAESRWVAVRLQVPYGAAAPG
                     SHPIHFNISAVSGDAQVSEKSAFLVPR"
     gene            3399..3650
                     /locus_tag="VGA59_03505"
     CDS             3399..3650
                     /locus_tag="VGA59_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein FixH"
                     /protein_id="HEX9718772.1"
                     /translation="MKQEQPGAAWWKHGYVWLVIAGPAAVVVAGLATVWLAARSPDPV
                     LAQERHRGVQIGKSTPDNRALMPAMQGRNHAATPTPARQ"
     gene            3685..3966
                     /locus_tag="VGA59_03510"
     CDS             3685..3966
                     /locus_tag="VGA59_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718773.1"
                     /translation="MWNWKLMWVAWPAFLSACALELVVFAMVDPQDLQWFGQPVALSR
                     QGVYTVSFFVFWAISGLSNALTALLGTPPAVVNGCPIAPGERPDGCPQQ"
     gene            complement(3911..4189)
                     /locus_tag="VGA59_03515"
     CDS             complement(3911..4189)
                     /locus_tag="VGA59_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718774.1"
                     /translation="MKARLNAIALRRAALALEIHVQRTAQRETLAVLRNDIAFAGLGL
                     VVGRVLVRRPWLRALALGALAAVAAGRLAPAIAAGSRPAVRRGQSDIR"
     gene            complement(4186..4551)
                     /locus_tag="VGA59_03520"
     CDS             complement(4186..4551)
                     /locus_tag="VGA59_03520"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX9718775.1"
                     /translation="MALGQSLSRLGANSLQMARQRLELASLDVEEELLRIGGLLAGAL
                     VTALMLALALAGAAAAFVIYFWEAARVAASLGVTGFFALAGALMVWRLARALRDKPRF
                     LAGTLAELDRDRQQWRDRA"
     gene            complement(4555..4875)
                     /locus_tag="VGA59_03525"
     CDS             complement(4555..4875)
                     /locus_tag="VGA59_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEX9718776.1"
                     /translation="MNDAPRSPDNGQKRLAEDFKAVVKDAEELLRHAARDAGDSYNEA
                     RARLEQSLKAARAELEVLEKAMLDGTRRAARATDGYVHDHPWEAVGIAAGVGLLLGML
                     ISRR"
     gene            5085..5735
                     /locus_tag="VGA59_03530"
     CDS             5085..5735
                     /locus_tag="VGA59_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX9718777.1"
                     /translation="MTDLFTDRATAGRALADRLQAYAGRDDVVVLALPRGGVPVAWEV
                     ARALNAPLDLLIVRKLGVPRQPELAMGAIASAGAMHVDPKIVSAYGVSARQLDEIIAA
                     ESLELIRRHALYCGKREPVPVEGRTVIVVDDGVATGASMNVALKALRSAKPAWIVAAV
                     PVAPPEAQELIGTAADEFVCVLSPPDFRSVGQYYRNFLQTGDDEVRELLARSLSAR"
     gene            5934..>6353
                     /locus_tag="VGA59_03535"
     CDS             5934..>6353
                     /locus_tag="VGA59_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9718778.1"
                     /translation="MPLSPVHILDSTTSARGDAANAAALLAGLLDSAMDAIITVDEKQ
                     TIVLYNRAAEKIFGYPRHEMLRQPLEKLIPGRFRANHAAHVEHFGATGVTSRRMGGLR
                     VVYGLRATGEEFPMDASISQLETPQGKLYTVILRDITE"
BASE COUNT         1041 a         2098 c         2145 g         1069 t
ORIGIN      
        1 ttctcgcacg aaaagatcga gaccagcaac ttcctgatga tcgtgctgat cctgctggtg
       61 gtcgccgtag gcggcctggt cgagatcgtc ccgctgttct tccagaagac gacgacccag
      121 gccatcgaag gcgtcaagcc ctataccgcg ctgcagctgg ccggccgcga cgtctacctg
      181 cgcgagggct gctacaactg ccactcgcag atgatccggc cgttccgcgc cgagacgatg
      241 cgctacggcc attattcggt ggcgggcgaa ttcgtctatg accatccatt ccagtggggc
      301 agcaagcgca ccggtccgga cctgcaccgc gtgggcggca agtacagcga cgaatggcac
      361 cgcatccacc tgaacaatcc gcgtgacgtg gtgcccgagt cgaacatgcc ggcctatcca
      421 tggctggaaa agggcgtcgt ggacgcagcc ggcatggccg ggcgaatgaa ggccttgcgc
      481 atggtcggcg tgccttacag cgacgaggag atcgccaagg cggccgccga cgtcaaggac
      541 aagaccgata tggacgcgct cgtcacctac ctgcaggcga tgggccgcgc cctgaaatga
      601 cccacccccg aagcgccttc ggcgatgccc cctcgggctt cgccctctcc ccctcgaggg
      661 ggccgacacc tgcggctcca aaccgacctg cgtcggtttg gacaggtcga ggaatggccg
      721 cgtctcgcgg ccatgcgggc ctgcaaggcc caccagcggc ccggcaaagc cggttccgcg
      781 gtggcccccg cattgaccgc agggtttcat gcgccgtggg tggcccaagc gcgatggatc
      841 actgactagg aaaagcaatg gatatggaca tcactaccct gcgcatcatc gccacgctcg
      901 cttccttcgg cactttcctg ggcattgcct ggtgggccta cgcgcgccac aaccgggacc
      961 gcttcgacga agccgcccgc attcccttcg aacaagactg acgcggagcc tgccatgagc
     1021 gatttcactg caaatttctg gtcggtctat gtggccggct tcacggtggt cagcatcatc
     1081 gcctgcctgc tgctgttgtg gatcacggca cgcagtaagg tcgtgcccaa tgccgacaac
     1141 accaccgggc atgtctggga cggcgacctg cgcgagtcga acaacccgat gccgcgctgg
     1201 tggatgtggc tgttcgtcat cacgatcgtg ttcggcctgc tgtacctggt ggcctatccc
     1261 ggcctgggca gctatccggg cgagctgaaa tggaccacga gcggcgagta cgccgcggag
     1321 gtcgagcagg ccaacaagga gctggcaccg atgtacgcgc agttcacggc caagaaggcc
     1381 gaggaactgg ccggcgacgc caacgcgatg gccgtcggcg agcggctgtt catgaacaat
     1441 tgcgcccagt gccacgggtc cgacgcgcgc ggcagcaagg gctttcccag cctggccgac
     1501 gcggactggc tgcacggcgg cgcgaccggg aagatcgccg agacgatcac gcagggccgc
     1561 cgcgggcaga tgccgccgat ggccgccgcc gtcggcacgc ccgaagacgt gaagaatgtc
     1621 gccaactatg tgctgagctt gtccaggagc ccgcacgatt cggtccgggc ccagctgggc
     1681 aaggcgaaat tcaccgcctg cgcggcctgc cacggcatgg atgggaaggg caatccggct
     1741 ctgggtgcgc ccaacctcgc ggacgacatc tggttgcacg gttggggcga gcaggccatc
     1801 gtcgccatgg tcaacaccgg caagatcaat gaaatgccgg cgcaggccgg caagctgacc
     1861 gaggcacaga tccacgtgct ggcggcctac gtctggggcc tatccaacaa gcccgcaccc
     1921 ggcaagctct gaggtgtcgg cagtgcagtc gacggccgct cgcaaggtga tccccatcgc
     1981 cgcagccgcg gccgaggccg aggagggcga atccctctac gaggcgcaga agaagatcta
     2041 tccgcgagcg gtgcggggcc tgttcgcgcg ctggcgctgg ggcctggtgt tcctgacgca
     2101 actcgtgttc tacggcctgc cctggctgca ctggggggag cgccaggccg tcctgttcga
     2161 cctggccgcg cggcgcttct acctcctggg ctatgtgctg tatccgcagg acttcatcta
     2221 cctcaccggg ctgctggtga tctgcgcgct gtcgctgttc ctgttcacgg cggtggcggg
     2281 acggctctgg tgcgggttcg cctgtccgca gacggtctac accgaaatct tcctgtggat
     2341 cgagcgcaag ctggagggcg accgcaacgc gcgcatgcgc ctggatgcgg cgccgatgtc
     2401 ggcgcgcaag ttcctgaaaa agggcggcaa gcacttcctg tggatggcgc tcgccatctg
     2461 gaccggcttc accttcgtcg gctacttcac gccgatacgg acgctgtggg cccagtcctt
     2521 cacgctgtcc ttcggcccct gggagtggtt ctgggtcaat ttctacgggc ttgcgacgta
     2581 tgggaacgcc ggcttcctgc gcgagcaggt atgcaagtac atgtgcccgt acgcgcgctt
     2641 ccagagcgcc atgttcgacc aggacacgct gatcgtcagc tacgacacgg gtcgcgggga
     2701 gccgcgtggc tcgcgttcgc gaaaggccga ccccgccgcg ctttcgctgg gcgcctgcgt
     2761 cgactgcagt ctgtgcgtcc acgtctgtcc caccggcatc gacatccgac aaggcctgca
     2821 gtacgagtgc atcggctgcg ccgcctgcgt cgacgtgtgc gacggcgtga tggacaagat
     2881 gaactacccg cgcgggctga tccgcttcac cacgcagaac ggcatggccc atggctggag
     2941 ccgctcgcag atctggcgcc acgtgctgcg cccgcgggtg ctggtctatg gcgccgtgct
     3001 ggtcgccttg tccgccgcgc tgctgggcag cctggtggtt cgcacgccgc tgaaggtgga
     3061 cgtggtccgg gaccgcgccg cgctctcgcg catcgttgcc gggggcaagc tggagaacgt
     3121 gtaccggctg cagatcatga acgccaccga gcaaccgcag cgctaccgta tatcggccga
     3181 ggggctggaa gggctggcgc tggcttcgga cgcggtggtc gccgtcgggc cggcggaatc
     3241 gcgctgggtc gccgtcaggc tgcaggtgcc ttatggcgcg gccgcacccg gctcgcatcc
     3301 gatccacttc aatatcagcg ccgtctcggg cgacgcgcag gtcagcgaga agtcggcctt
     3361 cctggtgccg agatagaaac tgagcgagag gaacaaggat gaagcaggaa caacccggcg
     3421 ctgcctggtg gaagcacggc tatgtatggc tggtgatcgc gggtccggcg gccgtggtcg
     3481 tggccgggct cgcaaccgtc tggctcgccg cgcgcagtcc cgacccggtg ttggcgcaag
     3541 aacgtcaccg cggtgtgcag atcggcaaga gcacgccgga caaccgcgcg ctgatgccgg
     3601 cgatgcaggg ccgcaaccac gcggcgacac ccacacctgc ccgccaataa tgatgtgaac
     3661 aggacccgat ccaaggagac agccatgtgg aactggaaac tgatgtgggt cgcgtggccg
     3721 gcgttcctct cggcctgcgc gctggaactg gtcgtgttcg cgatggtgga cccccaggac
     3781 ctgcaatggt ttggccagcc ggtggcgctg tcccgccagg gcgtctatac ggtgagtttc
     3841 ttcgttttct gggcgatcag cggcttgtcg aacgcactca cggcgctgct cggcacgccg
     3901 ccggcggtgg tcaacggatg tccgattgcc cccggcgaac ggccggacgg ctgcccgcag
     3961 caatagcggg ggccaagcgc ccggccgcga cagcggcgag ggcgccgagc gcgagagcgc
     4021 gcagccaggg acgccggacc aggacccggc cgacgaccag gcccagcccg gcgaacgcga
     4081 tgtcgtttcg caggacggcc agcgtttcgc gctgcgccgt gcgctgcaca tggatctcga
     4141 gggcgagcgc ggcgcgccgc agcgcgatcg cgttcagccg cgccttcacg ccctgtcacg
     4201 ccactgttgg cggtccctgt cgagctcggc gagggtgccg gccaggaagc gcggcttgtc
     4261 gcgcagggcg cgcgccaggc gccagaccat gagagcgccg gccagcgcga agaacccggt
     4321 tacgcccaag gaggcggcca cccgggcggc ttcccagaag taaatcacga aagccgccgc
     4381 ggcgccggcg agcgccagcg ccagcatgag cgcggtcacc agcgcgccgg cgaggagtcc
     4441 accgatgcgc agcaattcct cttcgacgtc gagcgaggcg agttcgagcc gctgccgggc
     4501 catctgcagc gaattggccc ccagccggct caaggactga cccagggcca tcggctatct
     4561 gcgcgagatg agcatgccca gcagaaggcc cacgccggcg gctataccga ccgcctccca
     4621 cggatggtcg tgcacatagc cgtcggtcgc gcgggcggca cgccgcgtgc cgtccagcat
     4681 ggctttttcc agtacttcga gctcggcgcg ggcggccttg aggctctgtt caagccgcgc
     4741 acgcgcttcg ttgtaactgt cgccggcgtc gcgcgcggca tgccggagca gttcttcggc
     4801 gtccttgact acggccttga aatcctcggc gagacgcttc tggccgttat cgggggaccg
     4861 cggtgcatcg ttcatggtgt cgctcctgtg ctgataccag tctagtcacg ggtcccgcga
     4921 acgaggttga tcgtcgtcaa gctatgctgc gagctctcac tcttgccgcg gcacgcacac
     4981 ctctgacgca gatcaccagg tccaggagcg acggttgatg tgcgtcaagc ctgcttttgg
     5041 cgggctgccc taacgtggct cacttgcaca gcaggagtgt tcccatgacc gatctcttca
     5101 ccgaccgcgc cacggctggc cgcgcgttgg ctgaccggtt gcaggcttat gccggccgcg
     5161 acgacgtcgt ggtgctggcc ttgccgcgcg gcggagtccc ggttgcgtgg gaagtggcgc
     5221 gcgcgctgaa tgcaccgctg gacctgctga tcgtgcgcaa gctcggtgtg ccgcggcagc
     5281 ccgagctggc catgggcgcg atcgcgagcg ccggcgcgat gcatgtcgat ccgaagatcg
     5341 tgtccgccta tggcgtgagc gcgcgccagc tggatgaaat catcgccgcc gagagccttg
     5401 aattgattcg gcgccacgcc ctgtattgcg gcaagcgcga accggtgccg gtcgaaggcc
     5461 gcacggtgat cgtcgtggac gacggcgtgg cgaccggcgc ctcgatgaac gtggcgctca
     5521 aggccttgcg cagcgcgaaa ccggcctgga tcgttgccgc cgttccggtg gcgccgcccg
     5581 aggcgcagga gctcataggc accgcggcgg acgagttcgt ctgcgtgctg agtccgccgg
     5641 atttccgcag cgtcggacag tactaccgca atttcctcca gaccggcgac gacgaggtcc
     5701 gcgagctgct ggcgcgctct ctttcggccc gatagccggt catgcatttc ctccccagcg
     5761 ccagctcggc tggcgtgcgt gctgtgggcc tgcgcctcgc ttgcctagcg catcggggcc
     5821 ttcccagacc gcgggtggat cggccggaac tatcccgcgc gattaagtaa tttttctact
     5881 tcagtttaac ttgctgtaga ttgggggcta ttttgcgcat gtcatactga gccgtgcccc
     5941 tttcccctgt ccacattctt gacagcacca cgagcgcgcg cggtgacgcc gcgaacgcgg
     6001 cggcgctcct ggccggactg ctcgattccg cgatggatgc catcatcacg gtcgacgaga
     6061 agcagacgat cgtcctgtac aaccgcgccg ccgagaaaat tttcggctat ccgcgccacg
     6121 agatgctgcg gcagccgctc gaaaagctca ttcccgggcg tttccgggcg aaccacgccg
     6181 cacacgtcga gcacttcggc gcgacggggg tgacgtcgcg ccggatgggc gggctgaggg
     6241 tcgtttacgg cctgcgcgcc accggggagg aattcccgat ggacgcgtcg atttcccagc
     6301 tggagacgcc ccaaggcaag ctgtatacgg tgatcttgcg cgacatcacc gag
//
LOCUS       DASWIY010000089         6081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2643567, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000089 DASWIY010000000
VERSION     DASWIY010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2643567"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..770
                     /locus_tag="VGA59_03540"
     CDS             12..770
                     /locus_tag="VGA59_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX9718779.1"
                     /translation="MTARSFFRYAVTLLMLLSGAVVAQEPGGREILDRVEQILWGNTV
                     QGDYEMTISTPRWQRMLGLRVWIERPKRSFVRIVSPAKEAGIGSLRLGSEMWNYLPNV
                     ERVIKIPPSMMLQPWMGSDFTNDDLVKQSSLLEDYTHKVLATVNVEAQAMYQVEAIAK
                     PEAAVVWGRILYWVRKSDFVPFKQEFYDERGELVRTMNYSDVRSMGGRRLPTRWEMRP
                     QGKPGNVTTIVLKNAVYDEPIAAEVFSQRNLQKP"
     gene            826..1512
                     /locus_tag="VGA59_03545"
     CDS             826..1512
                     /locus_tag="VGA59_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9718780.1"
                     /translation="MNTAVRLQGVSRIYRQGNVAVNALTQIDLEIRKGDFAVLVGPSG
                     SGKTTLLNLIGGLDTPSEGRIEVDGTEIGRMSRAQLSEVRLHKIGFVFQEFNLIPVLS
                     ALENVEFVMLLQGVGEAQRRERASALLTELGLDGMEHRRPNELSGGQQQRVAVARAIA
                     AEPIIVLPDEPTANLDSKAGAALMDLMRRLNEEKGVTFVFSTHDAMVVERARRVIHLR
                     DGRVESDERR"
     gene            1509..4091
                     /locus_tag="VGA59_03550"
     CDS             1509..4091
                     /locus_tag="VGA59_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9718781.1"
                     /translation="MSTPVRSLRSSAEAATGALRSRGAGTGRSILWMLAWRNVLRNRR
                     RSLITIASIALGVAALTFLWGFVDGMNRQMVENTTRYFAGDVQVHLKGYHGDPSLDLA
                     IADATPLLDAVSADPAVAAASLRLEGKALASRGDKSRGVMVVGIDPASEPRVTDLFKS
                     VTLGTALDGRAGGVLIGEKLAESLGVKVGEEIVLVGQAYDGSVASSRLPVRGVFRTQI
                     DELDGYLAVAPLAVVRDFLAAPGGATAIALRLRDRGALVAAQASLSQRVGARFEVVGW
                     PTLLPMVAVSVRYHEVMGYVVLTIFFVVVAAGVANPVLMAVLERTREFGIMLAIGTSQ
                     ARLLRLVLFEAMLLGAFGLLFGNAVGLALTAIFGTSGINLSAFEAGLRTMPGLADIVY
                     PVLQLQRSLMISMLVFGITAVVALYPALKAARLDPVAAIRGVAGNIGTQVASRYGKIA
                     RWPVFMLIAGRNLLRNPRRSAITAGGAAFAIVAYVFMYGYFDGFSEQLIDNATRYVTG
                     HAQVERPGFRRDLAAELAVGNPKPLLEALRRVPNVEAAAPRVQAQALASSATKSEGIM
                     LYGVDPVAERRLTFIHRAIVQGSALEGGNDRDIVLGRKLAEKLGLRLGEKVIVMAPAA
                     NGELGTAAYRIRGIFATESSSFDTAMAFVTLPAAQTLLALGDRVSTVNLRLAERDRLD
                     ATMAQLRAASIEAGLAFVPWPEMLPQVNQMVGLIRTIRAIVLGIFLVVVALAVMNTVF
                     MAVAERTREFGVMMALGTPPGAIVRMVLYESVALMAVASVIGYGLGIALVSWFGRNGL
                     DMSSLFRDYDAIPGLTGIAYPRVVMSSIVLPGVALFAGSVLVSLLPAMRAARLDPSKA
                     IRHA"
     gene            4084..5235
                     /locus_tag="VGA59_03555"
     CDS             4084..5235
                     /locus_tag="VGA59_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718782.1"
                     /translation="MRRAVGLAVLGLWSAWAGAAEEAGPGTAAWKFSGYYKNLLTRSE
                     TVFPAGAPYTADLNRLRLKIEGQPAANLAIDLQYDNEVLLGSYLRAAQFELQKALPRQ
                     TYWRAQSTYEDRSDFHGRHRLYRAVLTWSQGATDIRIGRQRIAWGTGRFFSPLDILNP
                     VNPITLERGERIGVDALLVEYKIDALSRVGAVYAPQHQSRWSSAAALWHGNHRGVDYS
                     VVAGRFGQQDVVGADFAGQIGQAGIRAEATHTRRPTGPGYTRVLVGVDYAFANTLTLS
                     GELYRDGSGARDPPVYDFPALFSGRVQNLGRRYAAAHAGYDITPLLKANVDLVTNLGD
                     RSFYFSPSLTWSIRPDIDATLGVQVFRGRAGTEYGAFKDGAFAQLVLFF"
     gene            complement(5272..5724)
                     /locus_tag="VGA59_03560"
     CDS             complement(5272..5724)
                     /locus_tag="VGA59_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718783.1"
                     /translation="MHRRKLTGFLLVHVLGAAALLGTNAFAAELAAQSSESDGVTIAV
                     KPVGISAGAAAWSFEVSLSTHTKDLNDDLVRTTYIVNRAGKKKEAPTGWKGDAAGGHH
                     RKGVLSFKAITPPPTALELRIQRVGEKAPRVFRWDLNCLCNDPKMHTS"
     gene            complement(5748..5891)
                     /locus_tag="VGA59_03565"
     CDS             complement(5748..5891)
                     /locus_tag="VGA59_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718784.1"
                     /translation="MKLMKGMHKKGDQHAPETSAREEAEPVAGMRADEGKPQYRVEDL
                     GKV"
BASE COUNT          992 a         1917 c         2078 g         1094 t
ORIGIN      
        1 agaggttagg aatgactgca cgttcatttt tccggtatgc cgtgacgctg ctcatgctac
       61 tgagcggcgc ggtggtggcg caggagccgg gcgggcgcga aatcctcgac cgtgtcgagc
      121 agatcctgtg gggcaacacc gtgcagggcg actacgagat gacgatcagc acgccacggt
      181 ggcagcgcat gctcgggctg cgggtgtgga tcgagcggcc caagcgctcg ttcgtgcgca
      241 tcgtgtcgcc ggccaaggag gcgggcatcg gttccctgcg gctgggttcc gaaatgtgga
      301 actacctgcc caatgtcgag cgtgtgatca agatcccgcc atcgatgatg ctgcagccct
      361 ggatgggctc ggacttcacc aacgacgacc tggtcaagca gagcagcctc ctggaggact
      421 acacgcacaa ggtgctggcc acggtcaacg tcgaagccca ggccatgtac caggtggaag
      481 ccattgccaa gcccgaggcg gcggtggtct ggggccgcat cctctattgg gtgaggaaaa
      541 gcgacttcgt gccgttcaag caggagttct atgacgagcg cggtgaactc gtgcgtacga
      601 tgaattattc cgacgtgcgc agcatgggtg gccggcggct gcccacgcgc tgggagatgc
      661 ggcctcaggg caaaccgggc aacgtcacca ccatcgtgct gaagaacgcg gtctacgacg
      721 agccgatcgc cgccgaagtg ttcagccagc gcaacctgca aaagccatga ggcgatgggc
      781 cgttcccgag acgaataccg cagcgcccag cgcggaggtt gctgaatgaa caccgctgtc
      841 agactgcaag gtgtgagccg catctaccgc caggggaacg tggcggtgaa tgccttgacg
      901 cagatcgacc tggaaatccg caagggcgac ttcgcggtcc tggtcggtcc ctcgggttcg
      961 ggcaagacaa cgctgttgaa cctgatcggc gggctggata cgcccagcga aggacgcatc
     1021 gaggtcgacg gcaccgagat cgggcgtatg agccgcgccc agttgtccga agtgcggctg
     1081 cacaagatcg gcttcgtgtt ccaggaattc aacctgatcc cggtgttgtc ggccctggag
     1141 aacgtcgaat tcgtgatgct gctgcagggc gtcggcgagg cgcagcggcg cgaacgcgcg
     1201 tctgcgctgc tgacagagct tgggctggac ggcatggagc atcgccggcc gaacgagctg
     1261 tcgggcggcc agcagcagcg ggtggcggtg gcgcgcgcga tcgccgccga gccgatcatc
     1321 gtgctgccag acgagccgac cgccaacctc gactccaaag ccggcgcggc gctgatggac
     1381 ctgatgcggc gcctgaacga ggaaaagggc gtgaccttcg tgttctcgac ccatgacgcc
     1441 atggtggtcg agcgggcccg acgcgtgatc cacttgcgtg atggtcgcgt cgaatccgat
     1501 gagcgcagat gagcacaccc gtacgctcac tgcgttcgtc cgccgaggcg gcgaccggtg
     1561 ccctgcgtag tcgcggcgcg ggcactggcc gctcgatcct gtggatgctg gcctggcgca
     1621 acgtgctgcg caacaggcgg cgctcgctga tcaccatcgc atcgatcgcc ctgggcgtgg
     1681 cggcgttgac cttcctgtgg ggcttcgtcg acggcatgaa ccggcagatg gtggagaaca
     1741 ccacgcgcta ctttgccggc gacgtgcagg tgcacctgaa gggctaccac ggcgacccga
     1801 gcctggacct cgccatcgcc gacgcgacgc cgttgctgga cgcggtgagc gccgatcctg
     1861 cagtggccgc ggcgagcctg cgcctcgaag gtaaagccct ggccagccgc ggcgacaagt
     1921 cgcgcggcgt gatggtggta ggtatcgacc ccgcgtccga accgcgcgtc accgatctgt
     1981 tcaagtcggt gacgctcggc accgccctcg acggccgcgc cggcggcgtg ttgatcggcg
     2041 agaagctggc cgagtcgctg ggggtgaagg tgggcgagga gatcgtgctg gtcggccagg
     2101 cctatgacgg ctcggtggcc agcagccgcc tgccggtgcg cggggtgttc cgcacccaga
     2161 tcgatgaact cgatggctat ctggcggtgg cgccgctcgc cgtcgtgcgc gattttctgg
     2221 ctgcccccgg tggtgcgacg gcgattgcct tgcgcctgcg cgatcgcggg gcgctggtcg
     2281 cggcccaggc gagcctgtcg cagcgtgtcg gcgcacgctt cgaggtggtt ggctggccga
     2341 ccctgctgcc catggtggcc gtgagcgtgc gttaccacga agtgatgggc tatgtcgtgc
     2401 tcacgatctt cttcgtggtg gtcgccgccg gtgtcgccaa ccccgtgctc atggcggtgc
     2461 tcgagcgcac gcgcgagttc ggcatcatgc tggcgatcgg caccagccag gcgcggctgc
     2521 tgcggctggt cctgttcgag gcaatgctgc tcggcgcctt cgggttgctg ttcggcaatg
     2581 ctgtcgggct cgccctgacg gccatcttcg gcacgtccgg tatcaatctc agcgcgttcg
     2641 aggccggctt gcgcacaatg cccggactgg cggacatcgt ctacccggtc ctgcagcttc
     2701 agcgcagcct gatgatttca atgctggtat tcgggatcac cgccgtcgtg gcgttgtacc
     2761 cggcactcaa ggcggcacgc ctggacccgg tagccgccat ccggggcgtg gcgggaaata
     2821 tcggcaccca agtcgcgtcg cggtacggca aaatcgcccg ctggcccgtg ttcatgctga
     2881 tcgccgggcg caacctgctg cgcaacccgc ggcgcagcgc catcaccgcg ggcggcgcgg
     2941 ctttcgcgat cgtcgcctat gttttcatgt acggctactt cgacggcttc agcgaacagc
     3001 tcatcgacaa cgccacgcgc tatgtcaccg gtcatgcgca agtcgagcgt ccgggcttcc
     3061 ggcgcgatct cgcggccgaa ctggcggtgg gcaacccgaa gccgctgctg gaagcgctgc
     3121 gccgggttcc gaacgtcgag gcggccgcgc cgcgcgtgca ggcccaggcg ctggcgagca
     3181 gcgcgaccaa gtcggagggc atcatgctgt atggcgtgga ccccgtggca gagcgccggc
     3241 tgactttcat tcaccgcgcc atcgtgcaag gttcggcgct ggagggaggc aacgaccgcg
     3301 atatcgtgct cggccgcaag ctggctgaaa aactgggttt gcggctgggc gaaaaggtca
     3361 tcgtcatggc gccggccgcc aatggagaat tgggcacggc ggcctaccgc attcgcggca
     3421 tcttcgccac cgagagcagt tcgttcgaca ccgcgatggc gttcgtcacg ctgccggccg
     3481 cgcagaccct gctggcgctg ggcgaccggg tatccaccgt caacctgcgg ctggccgagc
     3541 gggaccggct cgacgctacg atggcgcagt tgcgggcggc cagcatcgag gcggggctcg
     3601 cattcgtacc gtggccggaa atgttgccgc aggtgaacca gatggtgggc ctgatccgca
     3661 ccatccgcgc gatcgtgctg ggcatcttcc tcgtcgtggt ggcgctggcg gtgatgaata
     3721 cggtgttcat ggctgtggcg gagcgcacgc gtgaattcgg cgtgatgatg gcgctgggca
     3781 cgccgccggg cgccatcgtg cggatggttc tgtacgaatc ggtcgcgctg atggctgtcg
     3841 cttcggtgat cggctatggc ctgggaatcg cgctggtatc ctggttcggg cgcaacgggc
     3901 tcgacatgtc cagcctgttc agggactacg acgccatacc gggcctgacc ggcatcgcct
     3961 atcccagggt tgtgatgagc agcattgtct tgcccggcgt ggcgctgttc gcgggcagcg
     4021 tgctggtctc gctgcttccc gcaatgcgtg cggcaaggct cgacccatcg aaagcgatac
     4081 gtcatgcgta gggccgtggg cctggccgtg cttgggctgt ggtccgcgtg ggcaggggcg
     4141 gcagaggaag ccgggccggg gacagcggcg tggaagttct cgggctacta caagaacctg
     4201 ttgacgcgat ccgaaaccgt cttcccggcc ggcgcgccgt ataccgcgga cctgaaccgg
     4261 ctgcggctga aaatcgaagg ccagcccgcc gccaacctgg cgatcgacct gcagtacgac
     4321 aacgaagtgc tgctcggaag ctacctgcgc gccgcgcagt tcgagttgca gaaagcactg
     4381 ccgcggcaga cctactggcg cgcgcagtcc acctatgaag accgcagcga tttccacggg
     4441 cggcatcgcc tctaccgggc cgtgctcacc tggtcgcagg gcgcaaccga tattcgcatc
     4501 gggcgccagc gcatcgcctg gggcaccggc cgcttcttca gccctctcga tatcctcaac
     4561 ccggtcaacc ccatcacgct cgagcgcggt gaacggatcg gggtcgacgc gctgctcgtg
     4621 gagtacaaga tcgatgcgct gtcacgggtg ggcgcggtgt atgcgccgca gcaccagtcc
     4681 aggtggtcga gcgcagcggc gttgtggcat ggcaaccatc gaggtgtcga ctactcggtg
     4741 gtggccggac gtttcgggca gcaggatgtc gtgggtgccg acttcgcggg gcagatcggc
     4801 caggccggaa ttcgcgcgga agcgacgcac acccgccgcc ccaccgggcc aggctacacg
     4861 cgggtcctcg tcggtgtgga ttacgccttc gccaatacgt tgaccttgag cggtgagttg
     4921 taccgggatg gatcgggcgc gcgcgatccg ccggtctatg acttccccgc gctgttcagc
     4981 ggtcgggtac agaacctggg gcggcgatat gccgccgccc atgccggcta cgacatcacg
     5041 ccgctgctga aggcgaacgt cgacctggtg accaacctcg gcgacagaag cttctatttc
     5101 tcgccgagcc tgacctggtc gatccgcccc gatatcgacg cgacgctggg tgtgcaggtt
     5161 tttcgcggac gcgccggcac cgagtatggc gccttcaaag acggcgcttt tgcccagctg
     5221 gtcctgtttt tctgagagcg gcggcgccgg cctgagccgc ggtggccggg gtcaggacgt
     5281 gtgcatcttc gggtcgttgc aaaggcagtt caagtcccaa cggaacacgc gtggcgcctt
     5341 ctcgccgacg cgctggatgc gcaattcgag cgccgtgggc ggcggcgtaa tagccttgaa
     5401 gcttagaaca cccttgcgat ggtggccgcc tgcagcatcg cctttccagc cggtgggggc
     5461 ttcttttttc ttgcctgcgc gattcacgat gtatgtggtg cgcaccaggt cgtcgttcag
     5521 atccttggta tgagtgctga gcgaaacctc gaaagaccag gcagcggctc cggccgaaat
     5581 gcccacgggc ttgacggcaa tcgtcacgcc gtcgctctcg ctgctttgcg ccgccagctc
     5641 cgcggcgaag gcgttggtgc caagaagcgc cgccgcgccc aagacatgaa caagcaggaa
     5701 tccggtcagc ttgcgacggt gcattcggca tgctccttgg gggacgttca gacctttccc
     5761 agatcctcta cccggtactg tggcttgcct tcgtccgccc gcatcccggc cacgggttcc
     5821 gcctcttccc gggcgctcgt ttccggcgcg tgctgatctc ctttcttgtg cattcccttc
     5881 atcaatttca tgtcagccag gaaaccaatc ccgaaccctg cggctggcgc gatatatgtc
     5941 cagggcccgc ccaaagactg gcctacggcc aaaccaccaa acagacctac gcaacaaagc
     6001 atgtttgctt ctcctctttc aaccgttcaa ggaagattgg aaaccactca agggtggaca
     6061 cggccggtga atttgccggc t
//
LOCUS       DASWIY010000090         1769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1912732, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000090 DASWIY010000000
VERSION     DASWIY010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912732"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..552
                     /locus_tag="VGA59_03570"
     CDS             262..552
                     /locus_tag="VGA59_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718785.1"
                     /translation="MQMQKKHLWMILACCLIPLAVLAAVWLLRIPPNIILFFGLILLC
                     PLLHWLMMRGMLGHNHGAADAPNHAQGHVQYEPHPPADKLAEPKLTGASEQP"
     gene            589..1308
                     /locus_tag="VGA59_03575"
     CDS             589..1308
                     /locus_tag="VGA59_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX9718786.1"
                     /translation="MLTYATLKQQPKRLLALTGLARREFEEGLPVFAQALAEAEAQAK
                     PKPKKRQRAPGGGRKPGLRTVEDKLLFILVYTKTYPLQVVQGELFDMSQSSANAWIHM
                     LLPVLTATLDALGVMPARDGAQAAQHERRQGEPRDLTIDGVERRRQRPKDPKIQAIHY
                     SGKKKGHFDKNVLVVNTRSKRVSFLSPTLPGSVHDKALADHVDIQYPNATTLRSDLGF
                     YGYRPKVREHLQPKKSPAART"
     gene            1578..>1769
                     /locus_tag="VGA59_03580"
     CDS             1578..>1769
                     /locus_tag="VGA59_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718787.1"
                     /translation="MLEIEPVRPETEALTIRANLALLDVPADGDIALDLAGRRLGEAW
                     DPACGCTVDGLEVEFESAIL"
BASE COUNT          359 a          528 c          568 g          314 t
ORIGIN      
        1 attcagcctc gaaatggcgc ccgcaggggc gccgtttctg cgttgaggcc ggatgagtca
       61 tctctgttgt cgtaggcggc ttcccgccac gtgcccacgc cgtcgctgag ccccacgtgg
      121 taagccgaac ggcgtgacgg agatcacgcc aaacggcgct tggccgacct cagcagcccg
      181 cataagctgg agcgtgggtg cctgtgacta tggttctatg ttggcggcac ggcgcggcac
      241 aggccagaac cggaagattc catgcaaatg cagaagaaac acttgtggat gatactggcc
      301 tgctgcctga tcccgctcgc cgtcctggcc gcagtctggc tcttaagaat tccaccgaac
      361 atcatcctgt ttttcgggct gatcctcttg tgcccactgc tgcattggct gatgatgcgg
      421 ggcatgctgg gacacaacca cggtgcggcg gacgccccca atcatgctca ggggcacgtt
      481 caatacgaac cccacccgcc agcggacaaa ctggccgagc ccaagctcac tggcgcttcg
      541 gaacagccgt aacaatagac tctatcggaa ataggataag cttgggatgt gcttacctat
      601 gcgaccctca agcaacaacc gaagcgattg ttggccctga ctggactggc gcggcgggaa
      661 ttcgaggaag gcctgcctgt cttcgcacag gcgctggcgg aggcagaggc ccaagccaaa
      721 ccgaagccga aaaagcgcca gcgcgcgccg ggcggtgggc gcaaaccggg cttgcggacg
      781 gtggaagaca agttgctgtt catcctggtt tacaccaaga cctacccgct gcaggtggtg
      841 caaggcgagt tgtttgacat gagccaatcg agcgcgaatg cgtggatcca tatgctcttg
      901 ccggtgttga ccgccacgct ggacgcactg ggggtgatgc ccgcacgcga cggtgctcag
      961 gcggcccagc acgaacggcg ccagggcgag ccgcgcgacc tgaccatcga cggggtggag
     1021 cgccggcggc agcggcccaa agacccgaaa atccaggcga ttcattacag tggcaagaag
     1081 aagggccatt tcgacaagaa tgtgctggtg gtcaacacgc gtagcaagcg ggtgagtttt
     1141 ctgagcccaa ccttgccggg ctcggtgcat gataaggccc tggccgacca cgttgacatt
     1201 cagtatccga atgcgacgac cctgcgcagc gatttggggt tctatggcta ccgacccaag
     1261 gtgcgtgagc atctccaacc gaaaaaaagc ccagcggcgc ggacctgacg ccgggccagc
     1321 aacggcacaa tcgccatttg gcgcgaacac gggtgcgggt cgaacatgcg gtggcgggcg
     1381 tgaagatcag tcgcattgcc aaagaccact tccgtaacac ggctgacggg ctctccgatg
     1441 tcgtcatggc gattgcgtgc ggcctgcaca atctgcgccg cgctcgtcgg gccaggcgcc
     1501 atcgcggctc ggatgcttat ttccgataga gtctattata tagttggatg gtacaattct
     1561 gagggcacat cggcggtgtg ctcgaaattg agcccgtacg tccagagacc gaggccctta
     1621 ccatacgggc gaacctggcg ctgctggatg tgcccgcgga cggcgacatc gcgctcgacc
     1681 tggcgggccg gcgactgggc gaggcctggg acccggcgtg cggttgcacc gtggacggcc
     1741 tggaggtcga attcgaatcg gccatcctg
//
LOCUS       DASWIY010000091         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3017658, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000091 DASWIY010000000
VERSION     DASWIY010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3017658"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1541)
                     /locus_tag="VGA59_03585"
     CDS             complement(141..1541)
                     /locus_tag="VGA59_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX9718788.1"
                     /translation="MFTLDSKLPTPLVTQIVEGFRRMVEDGQLRAGTKLPSIRQFAHA
                     HEVSVYTVVDAYDRLVALGFFASRPHSGFFVRGRATGALAQTSIRGNYTFDSMWYMRR
                     IFENRALRSKPGCGWLPNDYLFSDGLKRSLRNVAADEEIDLGGYGEPKGFPPLRQLVR
                     DLLAEHEIVTTAEQILLTHGSSQALDLAARRLVRAGDAVLVDEPGYANLLSSLRFLRA
                     RLIGAPRTPQGWDLDVLEARVKEHRPKVFFTHPRLQSPTASTAQASHLHRVLQLAEKY
                     DFVIVENDIYVDLDPETRPSLASLDQLQRVIYISSFSKTISPNIRVGYLAAQADLLED
                     LAQMKMLSGLTSSEFTERLAYGALLDGRWRKHLKSVRERLAQAHQLSANTLLELGFEL
                     FCEPKAGMFLWARHPQLPSGAELSYKAAEHDIMLGPGHLFYTDLAPSPWLRFNVAFCT
                     DGAVFSFLEKEALPAG"
     gene            1715..3007
                     /gene="gabT"
                     /locus_tag="VGA59_03590"
     CDS             1715..3007
                     /gene="gabT"
                     /locus_tag="VGA59_03590"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX9718789.1"
                     /translation="MQRELHLTNAALMARRQAAVPRGVGHAHAIFAARAENAEAYDVE
                     GRRYIDFAGGIAVLNTGHRHPAVVEAVKAQLDLYTHTCFQVLPYEPYVALAERLNALA
                     PGDFPKKTLFLTTGAEAVENAIKIARAATGRPGVIAFGGGYHGRTLLTLSLTGKVAPY
                     KTGFGPFPGEIFHAAFPDPARGVSVDDSMASIEAIFRNDIEAARVAAIIVEPVQGEGG
                     FNVAPPELFRRLRELCDAKGILLIADEIQTGAGRTGTWFAVEQMGVAPDLITLAKSMA
                     GGFPISAVIGRASVMDAPAPGGLGGTYAGSPLGCAAALAVLDVFERENLLQRAHEVGL
                     RLVSALQKLAQRHPCICAVRGLGAMVAFEFSKNGDPNQPDAELAKRIMAEAAERGLIL
                     LTCGTWGNVIRILVPLTASDAVLDEGLSILGDCLNHCA"
     gene            3017..>3243
                     /locus_tag="VGA59_03595"
     CDS             3017..>3243
                     /locus_tag="VGA59_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9718790.1"
                     /translation="MSHATLAAVEPSHRESAGRAPSLTLLQPPLVSFSGVNKTYDGTA
                     LVVRDLNLEIRRGEFLTLLGPSGSGKTTTLM"
BASE COUNT          546 a         1105 c         1057 g          535 t
ORIGIN      
        1 cgccagcggt ttgccgtggc gaaaggtgtt gcgtgcgctg gcgaggcatg gatcccgggt
       61 cgagcccggg atgacaaatc cctctgccgg gatgacaaat ccctctgcct ggatgacaag
      121 tccctctgcc gcgcggacac ctaccccgcc ggcaacgctt ccttctccag gaacgaaaac
      181 accgcgccat ccgtgcagaa ggcgacgttg aaccgcaacc aggggctggg cgccaggtcg
      241 gtgtaaaaca ggtggcccgg gcccagcatg atgtcgtgct ccgccgcttt gtacgacagc
      301 tcggccccac tggggagttg cggatgcctg gcccagagaa acatgccggc cttgggctcg
      361 caaaacagct cgaatccaag ttccagcagt gtgttcgccg acagctggtg ggcctgggcc
      421 aggcgctcgc gaacgctctt gaggtgcttg cgccagcgcc catcgagcag ggcgccataa
      481 gccagccgct ccgtgaattc cgatgacgtg aggcccgaca gcatcttcat ctgcgccagg
      541 tcttccagca ggtcggcttg cgccgccagg tagccgaccc ggatgttggg cgaaatggtc
      601 ttggaaaagc tgctgatgta gatgacgcgc tgcagctggt ccaggctggc cagcgacggc
      661 ctcgtctcgg gatcgagatc gacatagatg tcgttttcca cgatcacgaa gtcgtacttc
      721 tccgcgagct ggagcacgcg gtgcaggtgc gatgcctgcg ccgtggaggc ggtggggctc
      781 tgcagccgcg gatgcgtgaa gaacaccttg ggccggtgct ccttgacccg cgcttccaga
      841 acgtccaggt cccagccctg cggtgtgcga ggggcgccga tcagccgggc gcgcaggaat
      901 cgcagcgacg acagcaggtt ggcgtagccc ggttcgtcca ccagcaccgc gtcgcccgcg
      961 cgcaccagcc tgcgcgcggc caggtccagc gcctggctgg agccgtgggt gagcaggatc
     1021 tgctccgcgg tggtgacgat ttcatgttcg gcaagcaggt cgcgcaccag ctggcgcaat
     1081 ggcggaaagc ctttgggctc gccgtagccg cccaggtcga tctcctcgtc ggccgccacg
     1141 ttgcgcaagc tgcgcttgag gccgtccgaa aacagatagt cgttgggcag ccagccgcag
     1201 cccggcttgc tgcgcagcgc ccggttctcg aagatgcgcc gcatgtacca catcgaatcg
     1261 aacgtgtagt tgccgcggat gctggtctgg gccagcgcgc ccgtcgcgcg gccgcgcaca
     1321 aaaaatccgg aatgcgggcg cgaagcgaag aatccgagcg ccaccagccg gtcgtaggcg
     1381 tcgaccaccg tgtacacgct gacctcgtgc gcgtgggcga actgccgtat cgagggcagc
     1441 ttggtcccgg cccgcaactg gccgtcctcc accatccggc ggaacccttc cacgatctgg
     1501 gtcacaagcg gcgtcggcaa tttgctgtcg agggtgaaca tcggcggcgc gaggtaacgc
     1561 gcggtgtaag ggtttatatg tactggtcgc ccggcctgca cagtgcatcg gctgtggctg
     1621 gcgatctgta tatgttagct ctgggccttg cctcctacag ttgccgccaa cccggacgag
     1681 aaaggcttgt ccgcgcaaga ccgaaaggac cgccatgcaa cgagagctcc acctgaccaa
     1741 tgccgccctg atggcgcgcc gccaggccgc cgtgccgcgc ggcgtcggcc atgcccacgc
     1801 catcttcgcc gcccgtgcag aaaacgccga ggcgtacgac gtggaaggaa ggcgctacat
     1861 cgatttcgcc ggcggcatcg ccgttctcaa taccgggcac cggcatccgg ccgtggtcga
     1921 ggcggtcaag gcccagctgg acctctacac gcacacctgc ttccaagtgc tgccctacga
     1981 gccctatgtg gcactggccg agcgcctcaa cgcgctggca cccggcgact ttcccaagaa
     2041 gaccctgttt ctcaccaccg gcgccgaagc cgtggaaaac gcgatcaaga tcgcccgcgc
     2101 cgcgacgggc cgccccgggg tgatcgcctt cggcggcggt taccacggcc gcaccctgct
     2161 gaccttgagc ttgacgggta aggtcgcgcc gtacaagacc ggcttcgggc cgttccccgg
     2221 tgagatcttc catgccgcct ttcccgaccc ggcacgcggc gtcagcgtcg acgattccat
     2281 ggcctccatc gaagccatct tccgcaacga catcgaggcg gcgcgcgtcg cggccatcat
     2341 cgtggaaccg gtgcagggcg aaggcggctt caacgtggcg ccgccggagc tgttccggcg
     2401 cctgcgcgag ctgtgcgatg ccaagggcat cttgctgatc gccgacgaga tccagaccgg
     2461 cgccggccgc accgggacct ggttcgccgt ggagcagatg ggcgtggcgc cggacctgat
     2521 cacgctcgcc aagtcgatgg ccgggggctt cccgatctcc gccgtcatcg gccgggcgtc
     2581 cgtcatggat gcacccgcac cgggcggcct gggcggaacc tacgccggca gtccgctggg
     2641 ctgtgccgcc gcgctcgcgg tgctcgacgt cttcgaacgc gagaacctgc tgcagcgcgc
     2701 ccacgaggtc ggcttgcggc tggtgtccgc gctgcaaaag ctggcgcaac gccatccctg
     2761 catctgcgcc gtacgcgggc tgggcgccat ggtggcgttc gaattctcga aaaatgggga
     2821 tccgaaccag cccgatgccg aactcgccaa gcgcatcatg gccgaagcag ccgagcgcgg
     2881 cctcatcctg ctgacctgcg gcacctgggg caacgtcatc cgcatcctcg tgcccttgac
     2941 cgccagcgat gccgtgctcg acgaggggct cagcatcctg ggcgattgcc tgaaccactg
     3001 cgcctgaatg cccgccatgt cacacgccac cctcgccgcg gtcgaaccca gtcatcgcga
     3061 atctgcaggg cgtgcgccat cgctcacgct cttgcagccg cccctggtga gcttcagtgg
     3121 cgtcaacaag acctacgatg gcaccgccct ggtggtgcgc gacctgaacc tggagatccg
     3181 gcgcggcgaa ttcctgacac tgctcggccc ctctggttcg ggcaagacga ccacgctgat
     3241 gat
//
LOCUS       DASWIY010000092         4146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3017701, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000092 DASWIY010000000
VERSION     DASWIY010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3017701"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VGA59_03600"
     CDS             complement(<1..593)
                     /locus_tag="VGA59_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9718791.1"
                     /translation="MTDAAPPPPRALGLTVAAAQLALALSWTVYMIFLPALAAQAGLR
                     PSAVILLLMLDQLVFVLADYACGVASDRVLALQARLGPWLVGLTLLSAAAFVSLPVLA
                     PEGSPALFIAVTLLWTATSSVLRAPPMNLIGRHAQRPAQPAMVALAMLGLGLAAALAP
                     WLALVLKGQDARLPFVVASLGVCAAALAMAAAEQRVRR"
     gene            960..1847
                     /locus_tag="VGA59_03605"
     CDS             960..1847
                     /locus_tag="VGA59_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9718792.1"
                     /translation="METTTRPGTPLRHRMLDDMRMRKFAEHTQDGYIRAVRKLAAFLG
                     RSPDTASTEDLRRFQLHLVDAGTGPVTINATITGLKFFFDITLGRPEVMAKMQHVRVP
                     RKLPVILSPEEVGRLIAAAPNLKHQAAMSVAYGAGLRVSEIVSLKVSDIDGQRMTLRV
                     EQGKGRKDRYAMLSPVLLERLRAWWRLGHAQGKILPDGWLFPGMDPTDHVTARQLNRA
                     VHLAAATAGLDKRVTPHVLRHSFATHLLEQKVDIRVIQVLLGHKRLETTALYAAVATD
                     LLRSVISPLDHTSDKPQPE"
     gene            1863..3044
                     /locus_tag="VGA59_03610"
     CDS             1863..3044
                     /locus_tag="VGA59_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEX9718793.1"
                     /translation="MQGRPALEVADIFRAHGPAWRDAQRGHLSLAQLKVMSAITHCRT
                     AALGGHVLRCDRCGLDQVSYNSCRNRHCPKCQSSAAKRWLDARQADLLPVEYYHVVFT
                     LPAPIADIAYQNKAALYGLLFDVAAETLLRIAADPKHLGASIGATLVLHTWGSALTHH
                     PHVHGIVPGGGLAPDGTRWVACRPGFFLPVRVLSRLFRRRFLEELQRLHEGGKLKFFG
                     EHAALADAAAFKAWLAPLRKCEWVVYAKRPFAGPQAVLAYLSRYTHRVAISNSRLLAM
                     DDLGVTFRWKDYRAKGKTRHKAMTLSPQEFMRRFLLHVLPGGFHRIRHYGLLANSNRR
                     DNLALARELLHVTPPPPDESAGEASIAPAPTFVCAHCGQAMVVLQVFLRDCSIRAPPA
                     S"
     gene            complement(3811..4131)
                     /locus_tag="VGA59_03615"
     CDS             complement(3811..4131)
                     /locus_tag="VGA59_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718794.1"
                     /translation="MSAQLPLAVASRPPRGLYVTALTWSFTLFNSVRLLAYLPTIWAI
                     YASGDSQQHSWFTWLTWAGANATMAAWLFEESGQRVTRAMMVNLCNAVMCLATLVLVL
                     GYRP"
BASE COUNT          679 a         1451 c         1396 g          620 t
ORIGIN      
        1 cggcgcaccc gctgctcggc cgccgccatg gccagcgccg cagcgcacac gcccaggctg
       61 gccacgacga agggcagccg cgcgtcctgg cccttgagca cgagcgccag ccagggcgcc
      121 agcgcggccg ccaggcccag ccccagcatc gccagcgcca ccatcgccgg ttgtgccggc
      181 cgctgggcat ggcggccgat caggttcatc ggcggtgcgc gcagcaccga cgaggtcgcc
      241 gtccacagca gcgtgacggc gatgaagagc gccggcgagc cctcgggcgc cagcaccggc
      301 agcgacacga aggccgccgc cgacagcagc gtgagcccga ccagccaggg ccccaaccgc
      361 gcctgcagcg cgagcacgcg gtccgaggcc acaccgcagg cgtagtcggc cagcacgaac
      421 accagctggt cgagcatcaa cagcaggatc accgccgacg gccgcaggcc ggcctgtgcg
      481 gccagcgccg gcaggaagat catgtaaacc gtccagctca gcgccagcgc cagctgggcc
      541 gccgccacgg tgaggccgag cgcacgggga ggcggtgggg cggcgtccgt catggccgcg
      601 cagcataggc cctggcggcg ggcccgagac gtcgccggca ccgggatgac cgcaatgtgg
      661 ccgagaccgt ggatacaagg tgctgccacg gccgaacgga accgttgcgg agctgccggc
      721 cgcgaccccg gatgtcggtt cacagactgg gtgcagccgt tcggatttgg cgggcgaatg
      781 gcggccattg cccaaagctg ccggccagcg tttgccggtc gagcgaccgg ttcactccgt
      841 gggactaaac cgctcgcgcg gtaggcgctc cggccaggcc ggtggtggtg cgttgaggct
      901 gcggctctgc gatgttggtg atcgaggcaa tccgcctcgt tcatcgactg gagcccacca
      961 tggagaccac cactcgccca ggcacgccgc tgcgccatcg catgctcgac gacatgcgca
     1021 tgcgcaagtt tgccgagcac acgcaggacg gctacatccg tgccgttcgc aagctggctg
     1081 cattcctggg ccgatcgccg gacaccgcca gcactgaaga tctccggcgc tttcaactgc
     1141 acttggtgga cgccggcacc ggtccggtga cgatcaacgc caccatcacg gggctgaagt
     1201 tcttcttcga catcacgctc ggccgacctg aggtgatggc caagatgcag catgtgcgcg
     1261 tgccgcgcaa gctgcccgtg atcctcagcc ccgaggaggt gggccggctg atcgcggcgg
     1321 cgccgaacct caagcaccag gcggccatgt cggtggccta cggcgccggg ctgcgcgtca
     1381 gcgagattgt gtcgctgaag gtgagcgaca tcgacggcca gcgcatgacc ttgcgcgtgg
     1441 aacagggcaa gggtcgcaag gatcgctacg ccatgctcag cccggtgctg ctggaacggc
     1501 tgcgggcctg gtggcggctg ggccatgccc agggcaagat cctgcccgac gggtggctgt
     1561 tccccggcat ggatccgacc gaccacgtga cggcgcgtca actcaaccgg gccgtgcatc
     1621 tggctgcggc tacggccggc ctggacaagc gggtgacgcc gcacgtcctg cgccacagct
     1681 tcgcgacgca cctgctggag cagaaggtcg acatccgcgt catccaggtg ctgctgggcc
     1741 acaagcggct ggagaccaca gccctttatg ccgcggtggc caccgacttg ctgcgcagcg
     1801 tgatcagccc gctggatcac acgtcggaca agcctcagcc tgagtaggcc gccgagatcg
     1861 cggtgcaagg gcggcctgcc ctggaggtcg ccgacatctt ccgcgcccac ggacccgctt
     1921 ggcgtgacgc ccagcgcggt cacctgagcc tggctcagct gaaggtgatg tcggccatca
     1981 cccactgccg cacggcggca ctgggtggcc acgtgctgcg ctgcgacagg tgcggtctgg
     2041 accaggtctc gtacaactcg tgtcgcaacc ggcactgccc gaagtgccag agcagcgcag
     2101 ccaagcgctg gctggatgcg cggcaggccg atctgctgcc agtggagtac taccacgtgg
     2161 tcttcacgct gccggcgccg atcgctgaca tcgcgtacca gaacaaggcg gcgctgtacg
     2221 ggttgctgtt cgacgtcgcc gccgagacgc tgctcaggat cgcggccgat cccaagcact
     2281 tgggcgccag catcggcgcc acgctggtgc tgcacacctg gggctcggcg ctgacccacc
     2341 atccgcatgt gcatggcatc gtgccgggcg gcgggctggc gccagacggt acgcgctggg
     2401 ttgcctgccg gcccggcttc ttcctgccgg tacgcgtgct ctcaaggctg ttccggcgcc
     2461 gtttcctgga ggaactgcag cggctgcacg agggtggcaa gctcaagttc ttcggcgaac
     2521 acgcagcgct ggccgacgct gcggccttca aggcctggct cgcgccgctg cgcaagtgcg
     2581 agtgggtggt ctacgccaag cggccgttcg cgggaccgca ggcggtgctg gcctacctgt
     2641 cgcgctacac gcaccgcgtg gcgatctcca acagccggct gctcgccatg gacgacctcg
     2701 gcgtcacctt ccgctggaag gactaccggg ccaagggcaa gacgcgccac aaggcgatga
     2761 cgctgagccc gcaggagttc atgcgtcgct tcctgctgca tgtcctgccg ggcggcttcc
     2821 accggatccg gcactacggc ctgctggcca acagcaaccg acgcgacaac ctggcgctgg
     2881 cgcgcgagtt gttgcacgtg acaccgccac cgcccgacga gtcggccggc gaggcgtcga
     2941 tcgcgccggc gccgaccttc gtctgtgccc actgcggcca ggcgatggtc gtgctgcaag
     3001 tcttcttgcg cgactgctca attcgggcgc cgcccgcatc atgaccgagc tccatcaccg
     3061 ccgaacaact cgacgccgaa ctctgcgtcc ggtggatggc tgcgtgcgct gctcgatcgc
     3121 cgctcttgga tgccccgcta ccggcggctg tcgcatcgca gaggcgcacc gcaagccgcc
     3181 tgcgttcgtc agcgcggtct cgaccatgcg tcgcgtgaag ctggctcgcg acgccagcgc
     3241 agaccaatcg ccatagccgc tgcgctcgac gaccaccgtg gcgttcgccg cggtttcctc
     3301 cctcgaggct tatgcaacac ctgcccggac gcagtgcggg cactcgacca cttgcgcgcg
     3361 agggcaggtg tcgcacaagc cttaacatga ggagccggtc gccgccggcg ggagtactca
     3421 cggtgtctgc catcaagccg acgttcggtt cggctgccgg cgctagcccg agcggccgct
     3481 gtatgcggga cagctgaccc cgggttgcga ccgatggggc tcgctgcttc aacgactgct
     3541 tccctgttgt gcgaactcgc agtaccgacc cgctgcagtc gttcggcgac ttgagaaccg
     3601 gtcgttcacc ccgggaacca acattggcag gacgcaaagt gaggcgatca ctacgggtcc
     3661 gcctatgctt ccccggcgca ttggcaagac ctcgccaaga ccaagctgac tttgcgctgc
     3721 ggggccgaag ggcttgggtc caatttcagc cagcgattgc cgatcaccag attcccttgg
     3781 tacggctcgg cgagagtccg atagtgcgaa tcaagggcga tagcccagga cgagcaccag
     3841 cgtggccagg cacatgaccg cgttgcacag attgaccatc atggcccgcg tcacccgctg
     3901 cccgctttcc tcgaacagcc acgccgccat cgtcgcgttg gcgccggccc aggtcagcca
     3961 ggtgaaccag gagtgctgtt gcgagtcgcc gctggcgtag atcgcccaga tcgtcggcaa
     4021 gtaggccagt aaccgcaccg agttgaacaa cgtgaacgac cacgtcagtg cggtgacgta
     4081 gaggccgcgc ggcggccggc tggcaaccgc gagcggcaat tgcgcgctca cggcctagcc
     4141 tccccc
//
LOCUS       DASWIY010000093        10708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3017956, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000093 DASWIY010000000
VERSION     DASWIY010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10708
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3017956"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VGA59_03620"
     CDS             <1..455
                     /locus_tag="VGA59_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718795.1"
                     /translation="GRRRRRFGGGISLQRVVVVVSLVDGVTVLSVALVPLPGPPIAPL
                     PVPVAGPPMEPELVLPDMSLLVVPGVVVPMPLLLDVEVSPLGAGVVVDELDDDDVSGV
                     GASSCLVQAPSETAAISARAAQEVREAFIGKLLMFESIKGSITALSAL"
     gene            complement(752..2074)
                     /locus_tag="VGA59_03625"
     CDS             complement(752..2074)
                     /locus_tag="VGA59_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX9718796.1"
                     /translation="MDFAAWSIVVGLLLIVMALSSTVLERLPLSTAMLYLVAGLAVSP
                     LGMHLLHADPHKNTVLLERLTEVVVLVSLFTAGLKLSPGLGDRRWLLPLRLALTSMVI
                     TVLGVALAAWLILDWPVGACILLGAILAPTDPVLASDVQVNRPGDRDRLRFALTGEGG
                     LNDGTAFPFVMLGLGLLGLHEIGSFGWRWVAVDLVWASLAGPAVGALLGLAVGRLVLY
                     LRREHKEAVGLDDFLAMGLIALSYGVAQLINAYGFLAVFAAGVALRRLEQSQAPRESS
                     RRAVEEAHAHPDKTLAQAVAVDPQHAPAFMAQALLGFNQQLERIGEVAVVVTIGAMLW
                     AVQWHAAAWWLVPLLFLLIRPLAVALGLFRSNSSTWQRWLIGWFGIRGVGSLYYLMFA
                     INHGVSPQLADPLIALALAVVVTSIIVHGISVTPLMAAYERAMKHRRR"
     gene            complement(2150..2986)
                     /locus_tag="VGA59_03630"
     CDS             complement(2150..2986)
                     /locus_tag="VGA59_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9718797.1"
                     /translation="MARVNNTAYALFSLGVLATAGFLYTAAIGWLWFRQERLLFEPTT
                     LPPDHVLATDPDVHELWIDVPGARLSAAHLRLPDPRGVILFLHGNSGNLTDCLVDLDA
                     FRQVNFDVVMFDYRGYGKSTGCIESEEQLRADVRAVWAEFARQYEGKRVVISGQSLGT
                     GLAAGLAAELCEDGRTPDLTLLVSPYSSGRALADELYPWVPRQVLRYPLHCVDHAVRL
                     QGPLILIHGENDELIGIHHSEALRTAVPNAQLLRIEGGGHNDLHKSPIFRKALAGALG
                     CL"
     gene            complement(3228..4052)
                     /locus_tag="VGA59_03635"
     CDS             complement(3228..4052)
                     /locus_tag="VGA59_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX9718798.1"
                     /translation="MTSSQHDKARLFSALHAAPGAFLIANAWDAGSARILAGLGFAAL
                     ATSSGASAGVLGRRDGRVTRDEALAHCRAIVAATDLPVSADLEKGFGDAPEAVAETIR
                     LAAQAGLVGGSIEDSTGNPDQPFYGMTQAVERVAAAVEAARSLPFPFTLTARAENFIR
                     GNPDLDDTIARLQAFERAGADVLFAPGLPDLAAVRAVCGAVNKPFNFMVAIRGKAFTV
                     PELEAAGVRRISLATSLYRVAMTGLVDAAREAKEQGTFTYLERSMTTAQLNGFMSG"
     gene            4172..5176
                     /locus_tag="VGA59_03640"
     CDS             4172..5176
                     /locus_tag="VGA59_03640"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HEX9718799.1"
                     /translation="MSQHQDSFPAYLISNTPAGVSADFTAFTDADLDPGDVTVRVQYA
                     SINFKDALAATGKGRIIRRFPCVGGIDASGVVQASGNPRFKPGDGVIVHSHGFGVSHH
                     GGYAARARVPADWVNHLPPGMSALEAVTLGVAGFTAALAIDLMELNGLAPGKGKVLVN
                     GATGGVASVSIDLLAQRGYQVVAVTGKPAEAPYLRTLGACEVIASADLPAGTKPLEPA
                     LWQGAVDSLGGSPLGALTRSMQRDGVIASIGNAAGLDFSTSVMPFILRGVRLIGVNSD
                     NEPAVREQVWKRLGTDLRPKHLAQIARVLPWAELPAAIEAVAGGKARGRTVIDVGTAK
                     "
     gene            5301..5510
                     /locus_tag="VGA59_03645"
     CDS             5301..5510
                     /locus_tag="VGA59_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEX9718800.1"
                     /translation="MDMESQEIKPVRSRRGFAAMDQQRQREIAAMGGRSAHASGHAHE
                     FTTEEARAAGKKRHQRTRENDSPSA"
     gene            complement(5540..6307)
                     /locus_tag="VGA59_03650"
     CDS             complement(5540..6307)
                     /locus_tag="VGA59_03650"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX9718801.1"
                     /translation="MIILSGAPNFRDVGGCAAAGGLMVRQGQLFRSDHLAALTAEEFA
                     GVLSTGVLRVCDLRGVAERASAPCALRNVTVHSLPIEPTIVQKLNDLLAAGHRLSRAD
                     VVALMQETYRGFVRGNTHRFAQLFSHLLESNEPTVFHCTAGKDRTGFAAALILRSVGV
                     SPEDVMRDYLLTNERLKPQPAARYGLAPEVVSVLRRVQPEFLNAAFEAVDEDYGGLEG
                     YFREGLGLREAQRERLGELYLEPRRGEAAAPPRGRSS"
     gene            complement(6526..6876)
                     /locus_tag="VGA59_03655"
     CDS             complement(6526..6876)
                     /locus_tag="VGA59_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEX9718802.1"
                     /translation="MDGALESLTHALRRFAAERDWDQFHSPKNLASALAVEAAELLEH
                     FQWLTEEQSRNLAEDKRIAVGHELADVFLYLLQIADKLQIDLVAAAERKLEVNAANYP
                     AAQARGTSKKYSEL"
     gene            7025..7981
                     /locus_tag="VGA59_03660"
     CDS             7025..7981
                     /locus_tag="VGA59_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HEX9718803.1"
                     /translation="MDYVPLPDNASRQLIDSTTIFDEFMRVQAQARQYAGGMYWKQQG
                     DYAYLVKTLPDNRQRRVGPRSPDTEKIYAEFTTRKRELEARMRSLRAALKDAERLNKA
                     LKVGRVPSLVVSVLQAIEDAGLGQHFTVVGTHALYAYEAAAGVRIVQGALATQDVDLL
                     WDARKRVRFMTDLAKLDTSVLGVLHQADATFQRKEGQNETAINAQGFEVDFLRRQPEG
                     DDPHPFRFSGDDGDLWPVQAVRASVLTNAPRFEHVIVAATGRMTLMRTIAPESFVEFK
                     RWMAEKVQKRPAPKRQRDLRQAEIVQRMIDEGLLMTRQGEPG"
     gene            complement(8104..8289)
                     /locus_tag="VGA59_03665"
     CDS             complement(8104..8289)
                     /locus_tag="VGA59_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718804.1"
                     /translation="MAPRSSKSSSTSKGSSSRAQAASKGGKSGMQKSERASSGRGSSK
                     LSSQGGEKKSSGSKSSK"
     gene            8583..10604
                     /locus_tag="VGA59_03670"
     CDS             8583..10604
                     /locus_tag="VGA59_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEX9718805.1"
                     /translation="MTEETIATSKSGGKKVERVLNARRDTLDFRDRMFEPTLVEVPSH
                     IKLDTYRYHKIPVLDQGTEGACTGFGLATVANYLLTLRKVEPDPVPVSPRMLYEMARR
                     YDEWPGENYSGSSARGAMKGWQKHGVCAEQQWKYQPGTRQAGGLTQARTADALRRPLG
                     AYQRVNHKDLVAMHTALAEVGILFATATVHQGWTQVDAKGVIPYPAPLSGGHAFAIVA
                     YDRHGFWIQNSWGDTWGADGFAQISYDDWLVNGTDVWVARLGAPVELRLNQSASIAHS
                     AAAGRSAAYSFSDLRPHVVSLGNDGALRPGGDYGTSPAELQQIFESDMPTAMAKWPEK
                     HVLLYAHGGLVDERSAIQRLADYRTAMLESHIYPISFIWHSDYWSTITNILQDAMRRR
                     RPEGVLGTAKDFLLDRLDDTLEIVARSLTGKASWDEMKENAQAASSPRGGACMALDHL
                     ARLRKRMPFKLHVVGHSAGSIFHAPLVRLLTAPAGPIASGYLAGKRGYGIGLDTCTLW
                     APACTAALFKEAYLPAVRDRSIGRLALFALDDKTEQDDHCARIYNKSLLYLVSRAFEE
                     STFGKGTPLLGMEKWLAADPVLRKIFIDGTADLVFSPNNDREGTTTAARAAAHGAFDD
                     DQATLKATLARILGSSGKAADEENAPDFEFHRSGSSMRRQREQLERQSA"
BASE COUNT         1879 a         3626 c         3425 g         1778 t
ORIGIN      
        1 gggggaggcg ccgaaggcgc ttcgggggtg ggatttcact tcagcgggtg gtggtggtcg
       61 tgtcgctggt ggatggggtc acggtgctgt ccgtggcgct cgtgccgctg cccgggccgc
      121 ccatcgcgcc gctgccggtg ccggtcgcag gcccgcccat ggagccggag ctggtgctgc
      181 ccgacatgtc gctgctcgtg gtgccgggcg tggtggtgcc catgccgctg ctgctggacg
      241 tggaggtgtc gccgctggga gccggcgtgg tggtggacga acttgatgat gacgacgtgt
      301 cgggtgtcgg cgcttcatcc tgcttggtgc aggcacccag cgaaacggcg gcaatcagcg
      361 ccagggcggc gcaggaagtc agggaggctt tcatcgggaa actccttatg ttcgaaagta
      421 tcaagggcag catcactgcc ctatcggcac tctaggcagg cacgccgccc agtttgtcgg
      481 ctgctgccgc ggatgtgtgt aggacagtca cagcggcagg gcttccgttt gaacactgtc
      541 gggcgcacgc gccgcgccca ccgtggcgca cgcgcggcgg aacgcggcaa gagatgggca
      601 aggcgcaggg aagctcagag ctcagcgttt gctgcccgtt tcgttaccgg tcttgccagt
      661 ctgtttcagt gcgctttcag ccgctgtacc gcggccgttg ttcccgcttt cgggggtgcc
      721 cgtgtcctcg cagagtcgac tcctccaatc ctcatcgccg cctgtgcttc atcgcccgct
      781 catacgccgc catcaggggg gtgaccgaaa ttccatggac gatgatggaa gtcaccacca
      841 cagccagcgc cagcgcgatc aacgggtcgg ccaactgggg cgacaccccg tggttgattg
      901 cgaacatcag gtagtacaac gagcccacgc cgcggattcc gaaccagccg atcagccaac
      961 gctgccaggt cgaactgttt gagcggaaca ggcccagcgc cacggccagt ggccggatca
     1021 acaaaaacag cagcgggacc agccaccagg cggcggcgtg ccactggacc gcccacagca
     1081 tggcgccgat ggtgaccacc acggccacct cgccgatgcg ttcgagctgc tggttgaagc
     1141 ccagcaacgc ctgtgccatg aacgcgggtg catgttgtgg atcgacagcc accgcttggg
     1201 caagggtctt gtcggggtgc gcgtgggctt cctccaccgc gcgccggctc gactcccgcg
     1261 gggcttgcga ttgctccaga cgccgcaggg ccactccggc cgcgaacacg gccaggaagc
     1321 cataggcatt gatcagctgg gcaacaccat acgacagggc aatcaatccc atggcgagga
     1381 aatcgtccag acccaccgcc tccttgtgct cgcggcgcag gtacagaacc agccggccca
     1441 cggccaggcc cagcaaggcg cccacggcgg ggccggcaag agacgcccac acgagatcga
     1501 ccgccaccca gcgccagccg aagctgccga tttcgtgcag acccagcagc cccagcccga
     1561 gcatgacaaa gggaaaagcc gtgccgtcgt tcaggccccc ttcccccgtg agcgcgaagc
     1621 gcagccggtc gcggtcaccc gggcggttca cctgcacgtc ggatgccagc accggatccg
     1681 tcggcgccag gatcgcgccg agcaggatgc acgcgcccac cggccagtcc aggatcagcc
     1741 aggctgccag ggccacgccc agcacggtga tcaccatcga cgtcagggcc agccgaagcg
     1801 gcaacaacca tcggcgatct cccaggccgg ggctgagttt gagccccgcc gtgaagagcg
     1861 acaccagcac caccacctcg gtgaggcgct ccagcagtac cgtgtttttg tgcgggtcgg
     1921 cgtgcagcag gtgcatcccc agcgggctca cggccaggcc cgccaccagg tagagcatgg
     1981 cggtcgacag gggcaggcgt tccagcacgg tgctgctgag cgccatgacg atcagcagta
     2041 atccgacgac gatcgaccag gcggcgaaat ccatgccgcc attgttgggg tggatcgccc
     2101 ttgcgccgtg tgaggaattg ccgcgacttg gcgcgcggga agccgcgttc taaaggcagc
     2161 ccagcgcccc ggccagcgct ttgcggaaga tgggggactt gtgcaagtcg ttgtggccgc
     2221 cgccttcgat gcgcagcagc tgcgcattcg gcaccgcggt gcgcaacgcc tcgctgtgat
     2281 ggatgccgat cagctcgtcg ttctcgccgt ggatcaaaat caatggccct tgcagacgca
     2341 ccgcatggtc cacgcagtgc agcgggtaac gcaacacttg ccgcgggacc cagggataga
     2401 gttcgtccgc cagcgcccgc ccgctgctgt agggcgacac cagcaaggtc aggtcgggcg
     2461 tgcgtccgtc ctcgcacaac tcggcggcga ggcccgcggc caaccccgtg cccagcgact
     2521 ggccggagat caccacccgc ttgccttcgt actgcctggc aaactcggcc cacacggcgc
     2581 ggacatcggc gcgcaattgc tcttcgctct cgatgcagcc ggtactcttg ccgtacccgc
     2641 ggtagtcgaa catcaccaca tcgaaattca cctgccggaa agcatccagg tccaccaggc
     2701 agtccgtgag gttgcccgaa ttgccgtgca ggaacaggat cacgccgcgc gggtcgggca
     2761 agcgcaaatg cgcggcggac aggcgcgcgc cgggcacgtc gatccacaac tcgtgcacgt
     2821 ccgggtcggt tgccagaaca tggtccggcg gcagcgtcgt cggctcgaac agcagccgct
     2881 cctgcctgaa ccagagccac ccgatggccg ccgtgtacag gaagccggca gtcgcgagca
     2941 ctcccagcga gaacaatgca taggcggtgt tgttgacccg ggccatgtta tttccccctg
     3001 cgggccgggt cgctcggacc gttgcggtcc gactggatct cgcgcacttc ctggggcatg
     3061 gcgtactcct tttgcggctc ggtctcggtt tcggtcgcaa cctgacttgt cccatatgct
     3121 gcttgcgccc ctgcctcagc cgtgtaggcg ctgatctgcg cattgcgtag gaactgagcg
     3181 ttaaacgttg tggcccgctg gccatccgtc gccctgcacg gccggcctca ccccgacatg
     3241 aacccattca actgcgcagt ggtcatggag cgttcgaggt atgtaaacgt cccctgctcc
     3301 ttcgcctcgc gcgccgcgtc caccaggcca gtcatggcca cgcggtacag cgacgtggcc
     3361 aggctgatgc gccgcacgcc cgccgcttcc agttcgggca ccgtgaaggc tttgcccctg
     3421 attgccacca tgaaattgaa cggcttgttc acggcgccgc acaccgcgcg cactgccgcc
     3481 aggtcgggca ggcccggggc aaagagcaca tcggctcccg ccctctcgaa tgcctgcagg
     3541 cgcgcgatgg tgtcgtccag atccggattg ccgcgaatga agttttcggc gcgcgccgtc
     3601 aaggtgaagg ggaatgggag cgagcgcgcc gcctccaccg cggccgccac gcgctccacg
     3661 gcctgcgtca tgccgtagaa cggctggtcg gggttgccgg tggagtcttc gatcgagccg
     3721 cccaccagcc cggcctgcgc cgccagccgg atcgtttccg cgacggcttc tggtgcatcg
     3781 ccgaagccct tctccaggtc ggccgatacg ggcagatcgg tcgctgcgac gatcgcgcgg
     3841 caatgcgcca gcgcctcgtc ccgcgtcacc ctgccgtcac gacgccccag tacgccagcc
     3901 gacgcaccgc tggaagtcgc cagcgcggca aaccccaggc ccgccaggat acgcgccgag
     3961 ccggcgtccc aggcattggc gatcagaaaa gcgccagggg ccgcgtgcag ggcgctgaaa
     4021 aggcgggctt tgtcgtgctg ggatgaagtc atcgcggtct ccttgctgga acaccccact
     4081 ttatcgaatc cgccttcggc gcccaccggc gaaggagcgc ccgcccaaag ccgcgccaca
     4141 atcgaagtct gcacatccct ccggaacttc catgagccaa caccaagact ctttccccgc
     4201 ctacctgatc tccaacaccc ccgccggcgt cagcgccgac ttcaccgctt tcaccgatgc
     4261 cgacctcgat ccgggcgacg tcaccgtgcg cgtgcaatac gccagcatca acttcaagga
     4321 cgcgctcgcg gccaccggca agggaaggat catccgccgc tttccctgtg tgggcggcat
     4381 cgacgcctcg ggcgtggtcc aggcctccgg caatccgcga ttcaagccgg gcgacggcgt
     4441 gatcgtgcac agccatggct tcggcgtctc gcaccacggc ggctacgcgg cccgggctcg
     4501 cgtgccggcc gactgggtga accatttgcc gccgggcatg agcgcgctgg aggccgtcac
     4561 cctgggcgtg gccggcttca cggcggccct ggcgatcgac ctgatggagc tcaacggact
     4621 ggcgcccggc aagggcaagg tgctggtgaa cggcgcgacc ggcggcgtgg cgagcgtgag
     4681 catcgacctg ctcgcgcaac gcggctacca ggtggtggcc gtgaccggca agccggccga
     4741 agcgccgtac ctgcggacgc tgggcgcctg cgaggtgatc gccagcgccg acctccccgc
     4801 cggtaccaag ccgctggagc cggcactgtg gcagggtgcc gtcgattcgc tgggcggctc
     4861 accgctgggc gccttgacgc gcagcatgca gcgcgatggc gtcatcgcca gcatcggcaa
     4921 tgccgccggc cttgacttct ccaccagcgt catgcccttc atcctgcgcg gcgtgcgcct
     4981 gatcggcgtc aattccgaca acgagccggc ggtgcgcgag caggtctgga agcggctggg
     5041 aaccgatctg cgcccgaagc acctggccca gatcgcccgc gtcctgccgt gggccgaatt
     5101 gccggccgcg atcgaggcgg tggccggcgg gaaggcgcgc gggcgcaccg tgatcgacgt
     5161 gggcaccgcc aagtgaatac ccctactact gtcgtgtagg acaacatcca attttgcggt
     5221 cacgccgccc acgcgaagct ctgccgcaac ataatttcca ggcgttcatc aacccgttga
     5281 attacttcac aggagacgtc atggatatgg aatctcaaga gatcaagccc gtccgctcca
     5341 ggcgcggttt tgccgccatg gaccagcaac gccagcgcga aatcgcggcg atgggcggcc
     5401 gttccgctca tgccagcggt catgcgcacg agttcacgac cgaggaagcg cgtgccgcag
     5461 gcaagaagcg gcaccagcgc acccgcgaaa acgactcacc gtcggcctga cggttgcgcc
     5521 gcggcgcccg ggtcgttcct caactggaac gaccacgcgg cggcgcggcg gcttctcccc
     5581 ttcgcggctc caggtaaagc tcacccaggc gctccctctg tgcctcgcgc aggcccagcc
     5641 cttcgcggaa gtacccttcg agcccgccgt agtcctcgtc caccgcctcg aaggcggcgt
     5701 tcaggaattc cggctgcacc cgccgcagta ccgacaccac ctccggcgcc agcccatacc
     5761 gtgcggctgg ttggggcttc aggcgctcgt tcgtcagcag gtagtcgcgc atcacgtcct
     5821 cgggcgacac gcccacgctg cgcaggatca gcgccgccgc gaatcccgtg cggtccttgc
     5881 cggcggtgca atggaacacc gtgggctcgt tggattcgag caggtgcgag aacagctggg
     5941 caaagcgatg cgtgttgccg cgcacgaagc cgcggtaggt ctcctgcatc agcgccacca
     6001 cgtccgcccg cgacagcctg tggccggcgg cgagcaggtc gttgagcttc tggacgatgg
     6061 tgggttcgat cggcagggaa tggacggtga cgttccgaag ggcgcagggg gccgaggcgc
     6121 gttcggcgac gccgcgcagg tcgcagaccc tgagcacgcc ggtggacagg acgccggcaa
     6181 actcttcggc tgtcaaggcc gccagatgat ccgagcgaaa aagctgccct tgacgaacca
     6241 tcaagccgcc ggccgccgca cagcctccga catcccggaa attgggcgca ccactgagga
     6301 tgatcatgac aaaacgtcct gatccgtcat cccgggccgt cctgatccgt catcacgggc
     6361 tgtcctgatc catcatcccg ggccgtcctg atctgtcatc ccgggctgtc ctgatctgtc
     6421 atcccgggct gtcctgatcc gtcgttccag gctgtcctga actgtcatcc cgggcttgac
     6481 ccgggatcca tggattggcc agctcgcaat gacaggcgac gcccatcaca gctcgctgta
     6541 cttcttgctg gtgccgcggg cttgcgccgc cggataattc gcggcattca cttcgagctt
     6601 gcgttcggcg gcagccacca ggtcgatctg gagcttgtcc gcgatctgca ggagatacag
     6661 aaatacgtcg gcaagctcgt gaccgaccgc gatccgcttg tcttcggcca ggttcctgct
     6721 ctgttcttcc gtgagccact ggaaatgctc cagcaactcc gcggcttcca cggcgagcgc
     6781 ggaagccagg ttcttcggtg agtggaattg gtcccagtcg cgctcggcgg cgaatcttcg
     6841 cagcgcatgg gtcagggatt cgagagctcc gtccattcaa ttcttctcga ttgcgttcct
     6901 ctgaacattg tgcccggtcc cgacctgtca tgttggcccc ccgtgagccc tcacctcaac
     6961 catataaggt attgattttc aataattagc cgtataaact tcaaatttaa taaaactacg
     7021 gccaatggat tacgtcccgc tcccggacaa cgcctcccgc cagctcatcg actctacaac
     7081 catctttgac gagttcatgc gcgtgcaggc acaggcgcgc cagtatgcgg gcggcatgta
     7141 ctggaagcag caaggtgact acgcgtacct cgtcaaaacg ctgcctgaca atcgccagcg
     7201 ccgcgtcggc ccgcgctcgc cggacaccga gaagatttac gcggagttca ccacccgcaa
     7261 gcgcgaactc gaagcccgga tgaggtccct gcgggcggcg ctgaaagatg cggagcgtct
     7321 gaacaaggcg ttgaaagtgg gccgcgtccc ttccctcgtc gtgtccgtgt tacaggccat
     7381 tgaggacgca ggactgggcc agcatttcac ggtggtaggt acccacgcgc tgtacgctta
     7441 cgaggccgcc gcgggcgtgc gcatcgtgca aggcgcgttg gcgacgcagg acgtggatct
     7501 cttgtgggat gcccgaaaac gggtgcgctt catgaccgac ctggcaaagc tggacacgtc
     7561 ggtgcttggc gtgttgcacc aagccgacgc cacgttccag cgcaaggagg ggcaaaacga
     7621 gacggccatc aatgcccaag gcttcgaggt ggactttctc aggcgtcagc ccgagggtga
     7681 cgatcctcat ccctttcgct tctcaggtga tgatggcgat ctgtggcccg tgcaggccgt
     7741 gcgggcgtct gtgctgacca atgcaccacg gttcgaacac gtaatcgtcg ccgccaccgg
     7801 ccgtatgacg ctgatgcgca ccatcgcgcc cgagtcgttt gtggagttca aacgctggat
     7861 ggccgagaaa gtgcagaagc gtcccgcccc caaacgccag cgcgacctgc gccaggcaga
     7921 gatcgtgcaa aggatgatcg atgaaggttt gctgatgacg cggcagggag agccaggatg
     7981 atcgaagcct ctgaccgatc aaagagatgc ctggcccgct tacgccggcc aggcatctta
     8041 cttttcgcag cgatggatcc cgggccgggc cggagcgcgc acggagtcag ggctagaaag
     8101 accctacttg ctgctcttgc tgccgctgga cttcttctcg ccaccctggc tgctgagctt
     8161 gctgctgcca cggccggagc tggcgcgttc gcttttctgc ataccggact tgccgccctt
     8221 gctcgcggcc tgggcacggc tgctgctgcc tttgctggta gacgacgatt ttgaactgcg
     8281 cggtgccata gatatctcct ttaatagtgc tgtgcggcca gcgcgctcgc gcaaagccac
     8341 aggctgactc tagaagcgac ttcgccactt tctgtcggtt gtgtttgcac attcatgtag
     8401 gacatccctt gtgcgacgcg ctcgcccgcg cggccggggc ccgacgctcc tccccacttt
     8461 ggaggatgac cgaaccgcgt cgtgctggca cgatggacgc gccgctgaaa gccagtcgag
     8521 cactacgaga ccatgtccct tccgcctccc cactcttttc agccctcacc gggagctaca
     8581 ccatgaccga agaaaccatt gcaaccagca agagtggcgg caagaaagtc gagcgcgtat
     8641 tgaacgcacg ccgcgacacc ctggattttc gcgaccggat gttcgaaccc acgcttgtgg
     8701 aggtgccgtc gcacatcaag ctggacacat accgctacca caagattcct gtcctcgacc
     8761 agggaaccga gggcgcctgc accggattcg gactggccac cgtggcgaat tacctgctga
     8821 cgctgcgcaa ggtggaaccc gatccggtcc ccgtcagccc gcgcatgctt tatgagatgg
     8881 cgcgccgcta cgatgaatgg ccgggcgaga actacagcgg ctccagcgcg cgcggcgcca
     8941 tgaagggctg gcaaaagcac ggggtatgcg ccgagcagca atggaaatac cagccgggaa
     9001 ccaggcaggc cggaggcctg acccaggcgc gcaccgccga cgcgctgcgc cgcccgctcg
     9061 gggcctacca gcgcgtgaac cacaaggatc tggtcgcaat gcatacggca ctggccgaag
     9121 tgggcatcct cttcgccacg gcgaccgtgc accaaggctg gacacaggtc gacgcgaagg
     9181 gcgtcatccc ttatcccgcg ccgctatcgg gcgggcacgc gtttgccatc gtcgcctacg
     9241 accggcacgg cttctggatc cagaactcct ggggcgacac ctggggcgcg gacggctttg
     9301 cccagatttc ctacgacgac tggctcgtca atggcaccga cgtctgggtg gcacggctgg
     9361 gtgcgccggt ggagctgcgg ctcaaccagt cggcatcgat cgcgcattcg gccgcggccg
     9421 gccgctcggc ggcgtactcg ttcagcgacc tgcggcccca tgtcgtgagc ctgggcaacg
     9481 acggcgcgct gcgcccgggc ggcgactacg gcacttcgcc cgccgaactg cagcagatct
     9541 tcgagtccga catgcccacg gccatggcca agtggcccga aaaacacgtc ctgctctatg
     9601 cgcacggcgg actggtcgac gagcgcagcg cgatccagcg cctggccgac taccgcaccg
     9661 cgatgctgga atcgcatatt tatccgatca gcttcatctg gcacagcgac tactggtcga
     9721 ccatcaccaa catcctgcag gacgcgatgc gccggcgtcg gcccgaaggc gtgctgggca
     9781 ccgccaagga ctttctgctc gaccggctgg atgacacgct ggagatcgtg gcgcgatcgc
     9841 tcacgggcaa ggcgtcctgg gacgagatga aggagaacgc gcaagccgcc agctcgccca
     9901 ggggcggcgc ctgcatggcg ctcgatcacc tggcccggct gcgcaagcgc atgccgttca
     9961 agctgcacgt ggtgggccat agcgccggtt cgatcttcca cgccccgctg gtgcgcctgc
    10021 ttacggcccc cgccgggccg atcgcgtcgg gctacctcgc gggcaagcga ggctacggca
    10081 tcggcctgga cacctgcacg ctctgggcgc cggcctgcac cgcggcgctg ttcaaggagg
    10141 cgtacctgcc ggcggtgcgc gaccgcagca tcggccgcct cgccttgttc gccctggacg
    10201 acaagaccga gcaggacgac cattgcgcgc ggatctacaa caagtcgctg ctgtacctcg
    10261 tctcccgtgc cttcgaggag agcacgttcg gcaaaggcac gccgctgctg ggaatggaaa
    10321 aatggctggc agccgacccg gtgctgcgca agatcttcat tgacggcacg gccgatctcg
    10381 tgttctctcc gaacaatgac cgtgaaggca cgacgaccgc ggcgcgtgcg gcggcccatg
    10441 gggcattcga cgacgatcag gccacgctga aagccacatt ggcgcgcatc ctcggcagca
    10501 gcggcaaggc ggcggacgag gaaaacgctc ccgacttcga attccatcgc tcgggttcgt
    10561 cgatgcgccg gcagcgcgaa cagctcgagc ggcaatcggc ttagctaagc aacttcctgg
    10621 ccagcagggc gggcgaatag acggtgacgc cgtcccaggt cttgccgtag ccggccccag
    10681 tgtggatccc gggtcaagcc cgggatga
//
LOCUS       DASWIY010000094         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_424636, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000094 DASWIY010000000
VERSION     DASWIY010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424636"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..468
                     /gene="rbfA"
                     /locus_tag="VGA59_03675"
     CDS             100..468
                     /gene="rbfA"
                     /locus_tag="VGA59_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX9718806.1"
                     /translation="MPKRTATPNRAFKVADQIQRDLTELIARELKDPRVGMVTIQAVE
                     VTPDYAHAKIFFSVLVGDPHECEEALNQAAGFLRNGLFKRLHIHTVPTLHFQFDRTTE
                     RAADMNALIAKAVSSRAKED"
     gene            526..1617
                     /gene="truB"
                     /locus_tag="VGA59_03680"
     CDS             526..1617
                     /gene="truB"
                     /locus_tag="VGA59_03680"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX9718807.1"
                     /translation="MKKLESAPHRSRIKRHPVHGVLLLDKPLGLSSNNALQKCKWLLR
                     AEKAGHTGTLDPLATGVLPICFGAATKFSRLQLDADKTYEAVVRLGVKTSTGDAEGEI
                     IEEREVPELTPARLAEICRQFTGPLRQVPPMHSALKKDGKALYEYARAGEHVERDARD
                     VVVRELDLAMAGTQSANSSLILRAKVSKGTYIRTLGEDIGEALGCGAHLTSLRRVATG
                     HFDISQCITLQALEAMGEEERLACLKPVVSLLPDHTRVTLDAENAGRFLSGLRRRGKW
                     PDRDRVAVYGPGPSPQPSPASGRGSIAEDRKEPPLADGRGSLHPLPLAGEGRGEGCLL
                     GTARIKGGELIPVRLLSPQEISQIMERNQ"
     gene            1614..3446
                     /gene="typA"
                     /locus_tag="VGA59_03685"
     CDS             1614..3446
                     /gene="typA"
                     /locus_tag="VGA59_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX9718808.1"
                     /translation="MSNKQIRNIAIIAHVDHGKTTLVDMLLRQSGTFRANEKVTERVM
                     DSNDLEKERGITILAKNCAVSWQGTHINIVDTPGHADFGGEVERALSMVDGVLLLIDA
                     VEGPMPQTRFVTKKALALGLKPIVVVNKVDRPGSRPDYVINAAFDLFDKLGATEEQLD
                     FPVVYASGLNGWASMEEGAPGEQWGKDLAPLFDTILTHVPAHEGESDAPLQLQISSLD
                     YSTYVGRIGVGRVNQGTIKVNQQVAVYEGVEATPAWAKVQQILKFDGLERVPAAEAGP
                     GDIVLISGIEQVGIGVTLTDPANPVPLPMLKIDEPTLTMNFCVNNSPLAGREGKYVTS
                     RQLWDRLEKELQANVALRVRDTDEEGIYEVSGRGELHLTILLENMRREGYELAVSKPR
                     VVFKEIKGERHEPIELLTVDIEEGHQGGVMQALGERKGDLVNMEPDGRGRVRLEYRIP
                     ARGLIGFQNEFLNLTRGTGLASNIFDGYEAYKGEIASRKNGVLISMDDGEIVTYALGK
                     LDDRGRMFVKAGDPVYEGMVVGIHSRENDLVVNAVRMKQLTNFRVSGKEEAIKITPPI
                     QLTLEYAVEFIEDDELVEITPKSIRLRKRHLKEHDRKRALRIVA"
     gene            3532..>3750
                     /locus_tag="VGA59_03690"
     CDS             3532..>3750
                     /locus_tag="VGA59_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9718809.1"
                     /translation="MKLTHTRAVFLMVGVTMMWSIAGVVTRQLEHARSFEITFWRSFF
                     TVASLMVILPLFQGRQVFARIRDGGPALW"
BASE COUNT          754 a         1161 c         1234 g          601 t
ORIGIN      
        1 aaatcaagga aatcgcacgt acgttgtgag ttgagcgtta tgcgtcgagc gtgacgtcca
       61 tcgaacgctc gacgctcatc gctcaacctc ttctgaacca tgcccaagcg cactgccacc
      121 cccaaccgcg ccttcaaggt cgccgatcag atccagcgcg atctgacgga gctgatcgcg
      181 cgcgagctga aggatccgcg cgtgggcatg gtcacgatcc aggccgtgga ggtcacgccc
      241 gactacgcgc acgcgaagat cttcttcagc gtcctcgtgg gcgacccgca cgaatgcgag
      301 gaggccctga accaggcggc ggggttcctg cgcaatggcc tgttcaagcg cctgcacatt
      361 cacacggtgc ccacgctgca tttccagttc gaccgcacga ccgaacgggc cgcggacatg
      421 aacgccctga tcgccaaggc ggtctcctcg cgggccaaag aagactaaac aattggggtc
      481 agattccaat tctcgttggg tctgcgccct gcgagctgcc tggccatgaa gaaattggaa
      541 tctgcacccc atcgttcgag gataaaacgc cacccggtgc acggggtgct gctgctcgac
      601 aagccgctgg gtctgtccag caacaacgca ttgcagaagt gcaaatggct gctgcgggcc
      661 gaaaaggcgg gacacacggg aacgctcgat ccgctggcga ccggggtgct gcccatttgc
      721 ttcggggcgg cgaccaagtt tagccgactg cagctcgatg ccgacaagac gtacgaggcg
      781 gtcgtgcggc tgggcgtgaa gacatcgacg ggtgacgccg agggcgaaat catcgaggaa
      841 cgtgaggtgc ccgagctcac gccggcgcgg ctggcggaaa tctgcaggca gttcaccggt
      901 ccgctgcgcc aggtgccgcc catgcacagc gcgctgaaga aggacggcaa ggccctgtac
      961 gagtacgcgc gtgcgggcga gcacgtcgag cgggacgcgc gcgatgtggt ggtgcgggag
     1021 ctggacctgg caatggccgg cacacagtcc gcgaattcct ccctcatcct gcgcgccaag
     1081 gtcagcaagg ggacctacat ccgcacgctg ggtgaagaca tcggcgaggc gctgggttgc
     1141 ggagcgcacc tcacctcgct gcgccgcgtc gccacgggcc acttcgacat ttcgcagtgc
     1201 atcacccttc aggcactgga agccatgggc gaggaagagc gcctggcgtg cctgaagccc
     1261 gttgtttcgc tgttgcctga tcacacgcgg gtcacgctgg acgcggagaa tgcggggcgg
     1321 tttttaagcg ggcttcgccg gcgcgggaag tggcctgatc gcgatcgggt cgcggtctat
     1381 ggcccaggac cctcacccca accctctccc gcaagcggga gagggagtat tgcagaggac
     1441 aggaaggaac cccctctggc agatggcaga ggaagtcttc accctctccc gcttgcggga
     1501 gagggccggg gtgagggctg cctcctcggc acggcccgca tcaagggcgg cgaactgatc
     1561 ccggtgcggc tgctgagccc gcaggagatt tcacagatta tggaaagaaa ccaatgagca
     1621 acaagcaaat ccgcaacata gcgatcatcg cccacgtgga ccacggcaag accacgctgg
     1681 tcgacatgct gctgcgccag tcgggcacgt tccgcgccaa cgagaaggtc accgaacggg
     1741 tgatggacag caacgacctc gaaaaagagc gcggaatcac catcctggcc aagaactgcg
     1801 ccgtgagctg gcagggcacg cacatcaaca tcgtggacac tcccggccac gccgacttcg
     1861 gcggtgaggt ggagcgggcg ttgtcgatgg tcgacggcgt gctgctgctg atcgacgccg
     1921 tggaaggccc gatgccgcag acccgcttcg tgaccaagaa agcgctggcg ctgggcctga
     1981 agcccatcgt cgtggtcaac aaggtggacc ggcccggctc tcggcccgac tacgtcatca
     2041 acgcggcgtt cgacctgttc gacaagctcg gcgcgaccga agagcagctg gatttccccg
     2101 tcgtctatgc ctcgggtctg aacggttggg cctcgatgga ggagggcgcg cccggcgagc
     2161 agtggggcaa ggacctggcg ccgctgttcg acaccatcct gacccatgtg ccggcccacg
     2221 agggcgagtc cgacgccccg ctgcaactgc agatttcatc gctcgattac tccacctacg
     2281 tcggacgcat cggggtgggc cgagtcaacc agggcacgat caaggtgaac cagcaggtcg
     2341 ccgtttacga aggtgtcgag gccacgccgg cgtgggccaa ggtgcagcag atcctgaagt
     2401 tcgacgggct cgagcgtgtg ccggcagccg aagcaggccc cggcgacatc gtgctgatca
     2461 gcggcatcga acaggtgggg atcggcgtga ccctcaccga cccggccaac ccggtaccgc
     2521 ttccgatgct caagatcgac gagccgacgc tcacgatgaa cttctgcgtc aacaactcgc
     2581 cgctggcggg ccgcgaaggc aagtacgtga ccagccggca gctttgggac cggctcgaaa
     2641 aggaactcca ggccaatgtc gcgctgcgcg tgcgggacac cgacgaggag ggtatctacg
     2701 aggtttccgg ccgcggcgag ctgcacctca cgatcctgct ggagaacatg cgccgcgaag
     2761 gctacgaact ggccgtttcc aagccgcgtg tggtgttcaa ggaaataaag ggcgaacgcc
     2821 acgagccgat cgaactgctg acggtggaca tcgaggaagg ccaccagggc ggcgtgatgc
     2881 aggccttggg cgagcgcaag ggcgacctgg tgaacatgga gccggacggc cgtggccggg
     2941 tgcgcctcga ataccgcatt cccgcgcgcg ggctgatcgg tttccagaac gaattcctca
     3001 acctgacgcg aggcacgggc ctggccagca acatcttcga cggctacgaa gcctacaagg
     3061 gcgagatcgc cagccgcaag aacggcgtgc tgatcagcat ggatgacggc gagatcgtga
     3121 cctatgcgct gggcaaactc gacgaccggg gccgcatgtt cgtgaaggcc ggcgatccgg
     3181 tgtacgaggg catggtcgtg ggcatccaca gccgcgaaaa cgacctcgtc gtcaatgccg
     3241 tgcgcatgaa gcagctgacc aatttccgcg tcagcggcaa ggaagaggcc atcaagatca
     3301 cgccgccgat ccagctgacg ctggaatacg cggtggagtt catcgaagac gacgagctgg
     3361 tggagatcac gcccaagtcg atccgcctgc gcaagcgcca cctgaaggag catgaccgca
     3421 agcgcgcgct gcggatcgtc gcctgataat tgtcattgcg ggctcgaccc gcaatccatg
     3481 acttcagaat tcatggatcc cggatcaagt ccgggatgac aggcacttct catgaaactc
     3541 acgcacacga gggcggtctt cctgatggtc ggcgtgacca tgatgtggtc gatcgcaggg
     3601 gtcgttaccc ggcaactgga gcacgcgcgc agtttcgaga tcaccttctg gcgcagtttc
     3661 ttcaccgtgg catcgctcat ggtgatcctg cccctgttcc agggccggca ggtttttgcg
     3721 cgcattcgcg atggcgggcc cgcgttgtgg
//
LOCUS       DASWIY010000095         6264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3018060, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000095 DASWIY010000000
VERSION     DASWIY010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3018060"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VGA59_03695"
     CDS             complement(<1..228)
                     /locus_tag="VGA59_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9718810.1"
                     /translation="MMKATPNFRTLDLNLLRVFDQVMAERNLTRAARNLAMTQPAVSN
                     ALNRLRDAVGDKLVARSGYGVEPTPRALALWP"
     gene            341..781
                     /locus_tag="VGA59_03700"
     CDS             341..781
                     /locus_tag="VGA59_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718811.1"
                     /translation="MTSFAHVDYPTEHAGVVRAEQAAQSLKKAVRGFDGNRAGASFLL
                     AAIVAALVVVASQVIDTWTEGHLLAAWIVMWAVVFAALALFAAPAKRAAALLRVGFQR
                     WNAARKQRAEDDQLWRLALTDARVMADISRAMTEGATREIKAYY"
     gene            796..987
                     /locus_tag="VGA59_03705"
     CDS             796..987
                     /locus_tag="VGA59_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718812.1"
                     /translation="MTTLVQPGLLSQVLSHIPSRVLKALDAWSYRVALRRAERRRLAH
                     DARARRAAAIVAQYRNWSH"
     gene            complement(1166..3034)
                     /locus_tag="VGA59_03710"
     CDS             complement(1166..3034)
                     /locus_tag="VGA59_03710"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX9718813.1"
                     /translation="MNSPVMQGLDLNCPVYVKNARLIAWVADMAALCKPDSIHWCDGS
                     DEEYRRLCRKLVDAGTFSKLDPGKRPNSFLARSDPSDVARVEDRTFICSQRKDDAGPT
                     NNWVAPAEMRATLQPLFDGCMRGRTMYVVPFSMGPLGSPIAHIGIELSDSAYVAVNMK
                     IMTRMGKAVYGALGVAGAFVPCVHTVGAPLESGLPDVPWPCNMTKYIVHYPETREIWS
                     YGSGYGGNALLGKKCFALRIASAMGRKAAETNGLGWLAEHMLILGVTTPEGRKYHVAA
                     AFPSACGKTNFAMLIPPRGFDGWKVTTIGDDIAWIKPGPDGRLYAINPEAGYFGVAPG
                     TNNETNPNCMATLDKDVIFTNVALTDDGDVWWEGMGPPPAHAIDWRGLDWTPRIAAET
                     GAKAAHPNARFTVAAVNNPALDPSWDDAKGVPIDAFVFGGRRSTTVPLVTEARDWVEG
                     VYMAATMGSETTAAIVGQVGVVRRDPFAMLPFTGYNMSDYFQHWLDLGRKLAASGRAL
                     PRMYTTNWFRKGADGKFVWPGYGDNMRVLKWMIDRIEGKAPGRENAFGLSPAYEDLNW
                     TGLDFGREQFETVMSIDRADWEKELRLHAELFRQLEYHLPRELIESKARLERRLAE"
     gene            3310..4077
                     /gene="modA"
                     /locus_tag="VGA59_03715"
     CDS             3310..4077
                     /gene="modA"
                     /locus_tag="VGA59_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9718814.1"
                     /translation="MRKRCFLGALALGPAALAALGTRALAPVRVAAASDLKFALAELA
                     RQYQSQAGSSVQLSLGSSGQFVQQIRQGLPVDLYMSADEDFVFQLADAGLTRDRGALY
                     ALGRIAAVVAAASPLVLDPALEGLRAAGAGIRHFAIANPEHAPYGRAAREALERLGLW
                     QWFRPKLVLGENIAQATQFVTSGAAQAGITALSLALAPEVARLARHVVLPDRLHAPLR
                     QRMVLLKSASAQASEFYAFLQTQAARAILRRYGFAFD"
     gene            4121..4789
                     /gene="modB"
                     /locus_tag="VGA59_03720"
     CDS             4121..4789
                     /gene="modB"
                     /locus_tag="VGA59_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX9718815.1"
                     /translation="MDWQATRVSLLLAVLTAVLLLPPGIALARWLAFSSWRGKPLAEA
                     CLMLPLLLPPTVIGFYLLLALGQGSPLGAWLARQLDLRLVFSFEGLLLASVLINLPFM
                     VQPIQRAFAAIPNSLREAAWVSGLSNWRTFVRIELPLAWPGLLAGVALTAAHTLGEFG
                     VVLMVGGSIPGQTRTLSIAIYDRVQAFDTAAAHVMALALVASSVVAVALVFAADRRRP
                     LQER"
     gene            4790..5890
                     /locus_tag="VGA59_03725"
     CDS             4790..5890
                     /locus_tag="VGA59_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9718816.1"
                     /translation="MLQVELRNATPMRLDASFDCAPGELVALVGPSGSGKTSLLRAVA
                     GLLKSRTLRGRVAVGQQAWFDSERALDLPPQQRHAGFVFQHYALFPHLSAIENVTLAA
                     GPATAADQFRALFERLGLAGLERRRPAQLSGGQQQRVALARALAREPKVLLLDEPFSA
                     VDAPARQALYRELAALRQTLSTPMVLVTHDLGEARRLADRVVILDAGRSLQTGTPAHV
                     FASPRNARVAQLIGIQNHFRGRFHKREPGWAALEWGQPCGIELRVLDKNRIPDGATVT
                     WVIAGERIDLLPDGAADGNTLRCKILEQLALGETSLCTLQPERLTEERVTLSLSTAQL
                     RSLGADAAGCLRLVIAPPAIHIMPQKDGSAGN"
     gene            complement(5912..6079)
                     /locus_tag="VGA59_03730"
     CDS             complement(5912..6079)
                     /locus_tag="VGA59_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718817.1"
                     /translation="MSSTEAEAPISHGAPPEPHEPHDPVESIIGAIPLVLPVMGGVLM
                     FLLAFIAVYMA"
BASE COUNT          954 a         2081 c         2138 g         1091 t
ORIGIN      
        1 gggccacagc gccagcgcgc gcggggtcgg ctcgacgccg taaccgctgc gcgcgaccag
       61 cttgtcgccc accgcgtcgc gcagccggtt cagcgcgtta ctcacggccg gctgggtcat
      121 tgccaggttg cgtgccgccc gcgtgaggtt gcgctcggcc atcacctggt cgaacacccg
      181 cagcaggttc aggtccaggg tccggaagtt gggggtggct ttcatcatta ccgtgaatga
      241 tagccatcac caatctaaat tggaaatata tcagtactaa ccctagtatt cgactacgca
      301 ctctggcaat ccagccacag cgtctagaag gggaaaaatg atgacaagct ttgcccatgt
      361 cgactatccg accgaacacg cgggtgtggt tcgcgccgag caggcggcac aaagcctgaa
      421 aaaagccgta cgcggcttcg acggtaaccg tgccggcgcc agcttcctgc tcgcagccat
      481 cgtcgctgcc ctggtggtgg tcgccagcca ggtgatcgac acctggaccg aaggccatct
      541 gctggccgcc tggatcgtga tgtgggcggt ggtattcgcc gcgctggcac tgttcgccgc
      601 gccggccaaa cgcgccgccg ccctactgcg ggtcggcttc cagcgctgga acgccgcgcg
      661 caagcagcgc gcggaagacg accagctctg gcggcttgcg ctgaccgacg cgcgcgtcat
      721 ggccgacatc agccgggcga tgaccgaagg cgcgacgcgc gagatcaagg cctactacta
      781 agacggagcg aaaaaatgac cacgctagtt caacccggct tgctgagcca agtcctgagc
      841 cacatcccat cgcgggtgct gaaggcgctc gatgcctggt cctaccgggt cgcgctgagg
      901 cgggccgagc ggcgacgcct cgcccacgac gcgcgagcac gcagggccgc tgccatcgtc
      961 gcgcagtacc ggaactggag ccattgaatc cgcccgtccg aagcggaata caactcggcg
     1021 cggtcggcgt agtcccggca atagtcggcc gcccgggcaa acagttcttc agccgcgcgc
     1081 gataagtcgg tagcctcggt gattaggcaa gcgtggcacc cctccgtgag cccccggcgc
     1141 gccgttgtgt ccacccctgc ggatcctatt cagcgagtcg cctttcgagt ctcgccttgg
     1201 attcgatcag ctcgcgcggg aggtggtatt ccagctgccg gaacagctcg gcgtgaagcc
     1261 gcagctcttt ttcccagtcg gccctgtcga tgctcatcac ggtttcgaac tgctcgcggc
     1321 cgaaatccag gccggtccag ttcaggtcct cgtaagccgg gctgaggccg aaggcgttct
     1381 cccggcccgg cgccttgccc tcgatgcggt cgatcatcca cttgagcacc cgcatgttgt
     1441 cgccgtagcc gggccagacg aacttgccgt ccgcgccttt gcggaaccag ttggtcgtgt
     1501 acatccgcgg cagcgccctg ccggaggcgg cgagcttgcg gccgaggtcc agccagtgct
     1561 ggaagtaatc cgacatgttg tagccggtga acggcagcat cgcgaacggg tcgcgccgca
     1621 ccacgccgac ctggcccacg atggcggcgg tggtttccga gcccatggtg gcggccatgt
     1681 acacgccctc cacccagtcg cgcgcttccg tcaccagcgg cacggtggtg gagcggcggc
     1741 cgccgaagac gaaggcatcg atcggcacgc ccttggcgtc gtcccaggac ggatcgagcg
     1801 cgggattgtt gacggccgcc acggtgaagc gggcgttcgg atgcgcggcc ttggcgccgg
     1861 tttcggcggc gatccgcggc gtccagtcca agccccgcca gtcgatcgca tgcgcgggcg
     1921 gcgggcccat gccttcccac cagacgtcgc cgtcgtcggt cagcgcgacg ttggtgaaga
     1981 tcacgtcctt gtccagggtg gccatgcagt tgggattggt ctcgttgttg gtgccgggcg
     2041 cgacgccgaa ataacccgct tccggattga tcgcgtacag cctgccatcc ggtcccggct
     2101 tgatccaggc gatgtcgtcg ccgatggtgg tgaccttcca gccgtcgaag ccacgcggcg
     2161 ggatcagcat ggcgaagttg gtcttgccgc aagccgaggg gaaagccgcc gccacgtggt
     2221 acttcctgcc ctcgggggtg gtgacgccca ggatcagcat gtgctcggcc agccagccca
     2281 ggccattggt ttcggcggct ttccggccca tggcggaggc gatgcgcaag gcgaaacact
     2341 tcttgcccag cagggcgttg ccgccatagc ccgacccgta ggaccagatc tcgcgggttt
     2401 cgggatagtg gacgatgtac ttggtcatgt tgcagggcca gggcacgtcc ggcagtccgc
     2461 tttccagcgg cgcgcccacg gtgtgcacgc agggcacgaa agcgccggcc actcccagcg
     2521 cgccgtacac cgctttgccc atgcgcgtca tgatcttcat gttgacggcg acataggcgc
     2581 tgtcggacag ctcgatcccg atgtgggcga tcggcgagcc cagcgggccc atggagaaag
     2641 gcaccacgta catggtgcgc ccgcgcatgc agccgtcgaa caggggctgc aaggtggccc
     2701 tcatttccgc gggtgccacc cagttgttgg tgggaccggc gtcatccttg cgctgcgagc
     2761 agatgaaggt gcggtcctcc acgcgggcca cgtcgctcgg gtcggatcgg gccaggaaag
     2821 agttgggccg tttccccgga tcgagcttgc tgaaggtgcc cgcgtcgacc agtttgcggc
     2881 acagccggcg gtattcttcg tcgctgccgt cgcaccagtg gatgctgtcg ggcttgcaca
     2941 gcgccgccat gtcggccacc caggcgatca gtcttgcgtt cttgacgtag accgggcagt
     3001 tcaaatccag tccctgcatt accggagaat tcatccaaag ctcctaaagt tggaccctct
     3061 gttcaacaag gcgggcgctc gggtggtgtg agcgcgcaac ttattgaaat tgggccttga
     3121 tacaggcgcc gggcgggttg cggggtcggc aacgtggtgg gcgtgaacgg gtatttcaca
     3181 tccgcgggtc ggctgggagg ccaattctag ggattgggga ccgcgcgcgg cttccgaccg
     3241 ggtgaataac aatgcaaaac atgcataggg ttatgcgacg gaatttttac tgtgccgatg
     3301 gggatttgcg tgcgtaaacg ctgctttctc ggcgcccttg ccctgggtcc tgccgcactg
     3361 gcggcgctgg gcacgcgtgc gctcgcgccg gtgcgcgtcg ccgcggcgag cgacctgaaa
     3421 ttcgccctcg cggaacttgc ccggcaatac cagagccagg cgggcagcag cgtccagctc
     3481 agcctggggt cgtcgggcca gttcgtccag caaatccggc aggggcttcc ggtggacctc
     3541 tatatgtcgg cagacgagga cttcgttttc cagctggccg acgccgggtt gactcgcgac
     3601 cgcggcgccc tgtacgcgct cggccgcatc gccgcggtgg tcgccgctgc ctcgccgctg
     3661 gtactcgacc cggcgttgga gggcttgcgc gccgccggcg ccggcatcag gcatttcgcc
     3721 atcgccaatc ccgagcacgc gccctatggc cgggctgcgc gcgaagccct ggagcggctt
     3781 gggctgtggc aatggttcag gccgaagctg gtcctgggcg agaacattgc gcaggcgacc
     3841 cagttcgtca ccagcggcgc ggcgcaggcc ggtatcacgg ccttgtcgct cgcgctggcg
     3901 ccggaagtgg cccggctggc gcgccatgtg gtgctgcccg atcggctgca tgccccgctg
     3961 cgccagcgaa tggtgttgct gaaatccgcc agtgcgcagg cgagcgagtt ctatgctttc
     4021 ctgcagacgc aggccgcccg cgccatcctg cggcgctacg gcttcgcgtt cgactagggc
     4081 cggcggcggc agcgcctcgc cacgcgtcac aatcgcggcc atggattggc aagccacccg
     4141 cgtatcgctg ctcctggccg tgctgacggc cgtgctgctg ttgccgccgg gcatcgcgct
     4201 ggcacgctgg ctggcattca gttcctggcg cggcaagccg ctggccgagg cctgcctgat
     4261 gctgccgctg ctgctgccgc cgacggtgat cggcttttat ctgctgctgg cgctcggcca
     4321 gggctccccg ctgggcgcct ggctggcgcg ccagctcgac ctgcggctgg tgttcagctt
     4381 cgaaggcctg ctgctggcca gtgtgctgat caacctgccg ttcatggtcc agcccatcca
     4441 gcgcgcgttt gccgccattc ccaactccct gcgcgaagcg gcctgggtgt ccggcctgag
     4501 caactggcgc accttcgtgc ggatcgaatt gccgctggcc tggccgggcc tgctggccgg
     4561 cgtggcgctc accgccgcgc atacgctcgg cgagttcggc gtggtgctga tggtgggcgg
     4621 cagtattccg gggcagaccc gcacgctcag tatcgcgatc tacgaccgcg tgcaggcgtt
     4681 cgacacggcg gccgcgcacg tgatggccct ggcgctggtg gccagctcgg tcgtcgcggt
     4741 ggcgctggtg ttcgcggctg accggcggcg gccgctgcag gaacgctaga tgctgcaagt
     4801 cgagctgcgc aacgccacgc ccatgcggct ggacgcaagt ttcgactgcg ctcccggcga
     4861 gctggtggcg ctggtcggtc cctcgggcag cggcaagacc agcctgctgc gggccgtggc
     4921 cggcctgctg aaaagcagga ccttgcgcgg ccgcgtcgcg gtcggccagc aggcctggtt
     4981 cgacagtgag cgcgcgctcg atctgccgcc ccagcaacgc cacgccggtt tcgtgttcca
     5041 gcattacgcg ctgttcccgc acttgagcgc gatcgaaaat gtcaccctcg ccgccgggcc
     5101 cgcaacggcg gcggatcaat tccgggccct gttcgagcgc ctgggcctgg ccggcctgga
     5161 gcggcgccgg ccggcccagt tgtccggcgg gcaacagcag cgcgtggcgc tggcccgggc
     5221 gctggcgcgc gagcccaagg tgctgctgct ggacgaaccc ttctcggccg tggatgcccc
     5281 ggcccgccag gctctttacc gggagctcgc ggcgctgcgg cagaccctgt ccacgcccat
     5341 ggtgctggtc acccacgatc tgggcgaggc gcggcggctg gccgaccggg tggtgatcct
     5401 ggatgccggt cgcagcttgc agaccggcac cccggcccat gttttcgcca gtccgcgcaa
     5461 cgcccgggtg gctcaactga tcggcatcca gaatcacttc cgcggccgtt tccacaagcg
     5521 cgagcccggc tgggccgcgc tggaatgggg ccagccctgc ggcatcgaac tgcgcgtgct
     5581 cgacaagaac aggattccgg acggcgcaac ggtcacctgg gtcatcgccg gtgaacggat
     5641 cgacctgctg cccgacggtg cagccgatgg gaacaccttg cgctgcaaga tcctggaaca
     5701 gctggctctg ggggaaacca gcctttgcac gctgcagccg gaacggctga ccgaggagcg
     5761 cgtgacgctc agcctctcca cggcccagct gcgctcactg ggcgcggacg cggcgggctg
     5821 tttgcggctg gtcatcgccc cgccggcgat tcacatcatg ccgcagaagg acggcagtgc
     5881 cgggaattga acgcgaacgc cgggcgccgc ctcaggccat gtaaacggcg atgaaagcga
     5941 gcaggaacat cagcacgccg cccatgacgg gaagcaccag cggaatggcc ccgatgatgc
     6001 tctcgaccgg atcatgcggc tcgtgcggct cgggcggggc gccgtggctg atgggcgctt
     6061 cggcttcggt gctggacatt gggttacctc tggggaatcg tgatcgggtc aatcctgaat
     6121 tttagccgct cgccggcggc gatccatcac ggcagcgcgt cagtgacgct ggcgcggcga
     6181 ctgcagcaac ccgatgttca tcattgctcc tgggcttgga aaccgccccc gtgcagcgcg
     6241 tcgaggacgg tctttatgtg ctcg
//
LOCUS       DASWIY010000096        13511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2281881, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000096 DASWIY010000000
VERSION     DASWIY010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 13511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13511
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281881"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /gene="metK"
                     /locus_tag="VGA59_03735"
     CDS             <1..958
                     /gene="metK"
                     /locus_tag="VGA59_03735"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX9718818.1"
                     /translation="RIGYDNTEYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDYLNSG
                     AGDQGLMFGYACDETPELMPAPIYYAHRLMERQAQVRKDGRLPFLRPDAKSQVTMRYV
                     DGKPHSIDTVVLSSQHSPEQSDGKHMLPSFIEAVIEEIVKPVLPAAWLKETRYLVNPT
                     GRFVIGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPSKVDRSAAYAARYVAKNV
                     VAAGLARQCQIQVAYAIGVAKPMNVTVYTEGTGLIPDDQIASLVHDHFDLRPKGIIQM
                     LDLLRPIYEKTAAYGHFGREEPEFTWERVDKAQALRAAAGLR"
     gene            complement(965..1870)
                     /locus_tag="VGA59_03740"
     CDS             complement(965..1870)
                     /locus_tag="VGA59_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718819.1"
                     /translation="MKIPWCRLAPAAAALLRLGLVFVLGFTGVSALAGSGELRTRYVE
                     LREQLRNNNFHRPLHIDSTQAGDTIRGDVYAVLDHSFREVSGALKDPADWCNILILPF
                     NTKYCHAVGSSAAANLLVRIGRRSDQPVEHAFRLDFVLRPVAATAGYFESRLDAGRGP
                     VGTHDYRIVVSAVPLDAGRTFMHLRYSYGYGFAGRLAMQAYLVTAGANKVGFTVTGRD
                     GNGEPLLIGGVRGAVERNVMRYYLAIDAHLASLAAPPEQRLGKRIATWFDATERYPRQ
                     LHEMDRASYTAMKRAESERQQAMIE"
     gene            complement(1867..2316)
                     /locus_tag="VGA59_03745"
     CDS             complement(1867..2316)
                     /locus_tag="VGA59_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718820.1"
                     /translation="MKYSLRKTPSHLHLAYKYGEAGDGLLGRNFTLEVEGRVLTLGID
                     LTPNFHTRNKSAASYLDAVNLVQNHHKLRYLQCTDNLLRTRLIRAWEQVENPKLRMCL
                     ELDQRGRFLYAVQPHSLFMGGIQFDVERVLEEDETPDRAHALELHHA"
     gene            2621..3691
                     /locus_tag="VGA59_03750"
     CDS             2621..3691
                     /locus_tag="VGA59_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEX9718821.1"
                     /translation="MAAEDKDLNRDPITDEPGSHPVGTGIGATGGAVAGAAAGTLGGP
                     VGVAVGGVIGAVVGGLAGKAAAEAVNPTAEEAHWREHYTREPYYEPGRGFDDYAPAYR
                     LGLSGRTRYEGDWDTAESRLASEWESARGGSSLSWPQAQPASRAAWDRIDASRPMQTD
                     WDRMSEEERAAMTHGRTDSRMSSGASDRPSGAAAVMGEMQSAGDSSGDRDDVIDALND
                     LVETCKDGEYGFKACAEQARRADLKSVFMQRADDCRGAAQELAEQIRGLGGKPEDGGS
                     AVGAVERGWVAVRAKLSTYEDLAVLEECERGEDNAVARYRKALRKPLPAGIKLIVERQ
                     LQGAQRNHDQIKTLRDQLRASA"
     gene            3859..6432
                     /gene="ligD"
                     /locus_tag="VGA59_03755"
     CDS             3859..6432
                     /gene="ligD"
                     /locus_tag="VGA59_03755"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX9718822.1"
                     /translation="MDERSPDALKTYRRKRDFDITPEPAAGGAANEQERAFVIQKHWA
                     SRLHYDLRLELEGTMKSWAVPKGPSFDPADKRMAVHVEDHPISYNTFEGTIPPGQYGA
                     GKVIVWDKGVWLALEDPHKGYREGKLKFELRGHKLRGQWTLVRIKNRADERQEPWLLI
                     KEKDGYARPALEFSVVDEMPDSVKRLADRPPRGATAARAGAATAQGASGPPSGARKAP
                     LPQALSPQLATLGDRPPANAGHWIYEIKFDGYRMLTRAQDGQVKLVTRNGHDWTRKLP
                     QLARALESMALPDGWYDGEIIMPGERVPADFQALQGAFDTARTAHVVYYLFDIPYCAG
                     YDLRDVPLLERRAVLQRVVQRKPHENVRFSAVFDAAPQDVLASACRLGLEGVIAKRKD
                     SAYVPRRSSDWVKLKCGLRQEFVIGGYTDPKGSRTGIGSLLLGVHDKSGSLRYAGNVG
                     TGFNEETLSDLRRRLDAIAAGSSPFASDAATPRNAHWVKPELVCEVAFGDWTREGRIR
                     HSVFRGVRTDKAPAAITREEPRHGAPVDRPAKGAKTAVRTAGMTRDKQAEATAVELPS
                     SLRVSNPDRVIDAASGTTKIALVRYYALVAPLMMEHLKGRPVALVRAPTGIGGEMFFQ
                     KHAERHQMPGVEQLDPAFYPGHPPMLEVATARGLLSAAQMNVIEFHTWNAVKSAIGKP
                     DRMTFDLDPGEGVKWLQVQQAAQLVRGFLAELGLAAFLKTSGGKGLHVVVPIRRQLDW
                     DSVKDFSQAIVRHLARAIPQRFVAKSGAANRVGKIFIDYLRNGFGATTVSAWSARARP
                     GLGISVPVRWDELDGLRGGDHWTVGTVHTRLDQGNAPWDDYEAGRKGLAAAMRALGFE
                     R"
     gene            complement(6466..7122)
                     /locus_tag="VGA59_03760"
     CDS             complement(6466..7122)
                     /locus_tag="VGA59_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718823.1"
                     /translation="MPNARLAEAARYTLLRRLAPSMRHHLVVNLQPIGMIYEVMERRL
                     RAPEPDLGEVHDSAQKINGFARAALNSCLDVVSWLAPEDGAVSTVDEGVRECLGLLAT
                     SLTFRGYSLRNDVGSVPGEIGRSAIRNVLTAAIIHMTDETPPVAGLVLSAQASPAALG
                     LTLTLEPTEGNKGFATETPYRPLAWADLEALAAAEGVGLTREGRQRVGLSIPWAGARG
                     "
     gene            8045..8260
                     /locus_tag="VGA59_03765"
     CDS             8045..8260
                     /locus_tag="VGA59_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718824.1"
                     /translation="MLEFVRSLRRRRDFWTRLPAFALAFLVAEIFYKFKSFSVECAAF
                     LVTWLIIDVIVDQLAKRLEPPKGRSLQ"
     gene            8413..9117
                     /locus_tag="VGA59_03770"
     CDS             8413..9117
                     /locus_tag="VGA59_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718825.1"
                     /translation="MNTPVWPKAFPAGIADPSGQFALVRQSNGRLMAVRLHDGNVVWR
                     QRHDVALQPLLVDSRQAVALSTSHAQVFAFSLHTAAAGAEWVSARLPWPEWATDANAQ
                     SVQSDCDAIWLGQDMGLRWELRLLYRGGAKPGPARPMGQTATGTCRVDLRSGGISSLP
                     TWPEGPGLLGWEPSQDPMVVAQCRINDREYRLTTRHEGSTVITSISARDVLTGHVFWE
                     VPLDEVVPRGPPPLRQ"
     gene            9179..11629
                     /locus_tag="VGA59_03775"
     CDS             9179..11629
                     /locus_tag="VGA59_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718826.1"
                     /translation="MAITFQDVAQKVGVSVELVELLCATRGTTLAGLVNIPEGGLRRA
                     IRRLTYPDLARARHEHLLKIARDDDGRIPPNAVDQALTELMALRKRLPQRAMVAGLPV
                     AKEVTSLELLGAIGPVVPMAAGLAPNRWRWLGPGNVGGRTRAIAIHPVDSQRMLAASA
                     GGGVWFTQDGGARWDPVDDFMANLAVCCLAVDPKDPNQVYAGTGEGFGNGDALRGAGI
                     FRIVGGNQWKQLSATQHFPAVNRIAVSADGKVMLAATPEGLQRSVDAPRARWRKVLAA
                     PLADVKFHPANSKLAIAGGLEDGQAYFSKNKGRTWQLGTHATAWSGRVELAYARKNPN
                     VVYASVQGDTGELWRSDDGGKTYQRRRALDPDGQPAQFLGDQGWYGNTVWAGDPGDEN
                     LVLVGGINLWRSVDGGDTLAEISTWWDSRSVHADHHCIVSHPGYDGTMNRTVFFGHDG
                     GISKATDIRVPGSEAQPPYVQGWVELNNNYGVTQFYGGAVHSATGRIVAGTQDNGSLC
                     LDPGEGTDQWHSWFGGDGGWCASDPTDPQVFYGEYVFLNIHRNMDGCATSDQQGDRYI
                     SGQFWDATRRDWAWKPVPFQISDARTQDALFIAPFVLDSRQPDRLLGGGLSLWETKDA
                     KAPNTPTSGPRWSRIKPSTGRFISAIAISPANSDVVWVGHQDGAVFVTTTGTSGSPAW
                     QRCPDTGPGGLTPKRYCTRIVPHPTDLKTAYVTFGGFNHDNVWQTQDSGSTWAPVSGN
                     LPAAPVYSLAIHPRRLNFIYVGTEVGIFGSEDSGVSWSPTNEGPANVSVNELFWNGQT
                     LVCASHGRGMFSIDLKGV"
     gene            11739..12788
                     /locus_tag="VGA59_03780"
     CDS             11739..12788
                     /locus_tag="VGA59_03780"
                     /inference="COORDINATES: protein motif:HMM:NF026913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEX9718827.1"
                     /translation="MKQPDTHAIERTVVTQKNNISRDFLDTVLDDLGERPDLLPTVFR
                     REVQLAAGLASNARSAKKAAPELIRAARSAALMFVHAWHRNKSTFIPLDARLDVELQG
                     EVDPSFVSAPAWIEAMWCALACGDVFAQQWLATVPPSRLAPAGVQHGRYAFALGEFLR
                     SLVTRDGRHGEWLVQATEASDDDDGDPLLEATRDWADGIDFPALRAAFHLLDEDQAGF
                     EQALTELHEQHKRYWGNGENRLAVDGLLSLRGCALLRLGAQLKIKVGFASGYMPPAVW
                     QAPAVPHPVRCPYCVVPLAAGARECGPCGRAIQDAPIELAAAATAGPRTPCKSCAYPL
                     HKLAVLCPQCRTARR"
     gene            complement(12798..>13511)
                     /locus_tag="VGA59_03785"
     CDS             complement(12798..>13511)
                     /locus_tag="VGA59_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718828.1"
                     /translation="MRLNPETGRLKDPTLVDDPVPDIRALKENRQAVPVDDLKLSKAD
                     KQRMKDLLAKRDKARADRDKALGKGDEEAAAGHARDVNEASRQLGEEHAKAYMKEKYP
                     DFEQLYPSDPTKPSRAGDFDQVWVKYGKNKRGKKVVVQVIVIEAKGGAGTLGTRKAGG
                     LVVQQGTGAYFESIIASMEKGTPEMNRIAAMLKALKPSEIEYKLVIAPIEMTRDAKPR
                     SQVLAVEVADFNLAKTLKP"
BASE COUNT         2311 a         4375 c         4523 g         2302 t
ORIGIN      
        1 gcgcatcggc tacgacaaca ccgaatacgg catcgactac aagggttgcg ccgtgctggt
       61 ggcctacgac aagcagtcga acgacatcgc gcagggcgtg gaccatgcga gcgacgacta
      121 cctgaacagc ggcgcgggcg accagggcct gatgttcggc tacgcctgcg acgagacgcc
      181 cgagctgatg cccgcgccga tctactacgc gcatcggctg atggagcgcc aggcccaggt
      241 tcgcaaggac gggcggctgc ctttcctgcg cccggacgcc aagagccagg tgaccatgcg
      301 ctatgtcgac ggcaagcccc acagcatcga caccgtggta ctttccagcc agcacagccc
      361 ggagcagagc gacggcaagc acatgctgcc ctcgttcatc gaggccgtga tcgaggagat
      421 cgtcaagccg gtcctgccgg ccgcatggct gaaggaaacg cgctacctgg tcaaccccac
      481 cggccgcttc gtgatcggcg gtccgcaggg cgattgcggc ctgaccgggc gcaagatcat
      541 tgtcgacacc tacggcggtg cctgcccgca cggcggcggc gcgttctcgg gcaaggaccc
      601 gagcaaggtc gaccgctcgg ccgcttacgc cgcgcgttac gtcgcgaaga atgtggtggc
      661 ggcgggcctg gcgcgccagt gccagatcca ggtcgcctac gccatcggcg tcgccaagcc
      721 gatgaacgtg accgtgtaca cggagggaac gggcctcatt cccgacgacc agatcgcgtc
      781 cctcgtgcac gaccatttcg acctgcggcc caagggcatc atccagatgc tggacctgct
      841 gcgcccgatc tacgagaaga ccgccgccta cggccatttc ggccgcgagg agcccgagtt
      901 cacgtgggaa cgggttgata aagctcaggc gctgcgtgca gcggcaggcc tcaggtaaac
      961 gggcctattc gatcatcgcc tgctgccgct cggactccgc acgcttcatc gccgtgtagc
     1021 tggcacggtc catttcgtgc aactggcgcg gatagcgctc ggtcgcgtcg aaccaggtcg
     1081 cgatcctttt gcccagccgc tgctcgggcg gcgcggcaag cgacgccagg tgcgcgtcga
     1141 tggccaggta gtagcgcatc acgttgcgct cgacggcacc gcgaaccccg ccgatcaaca
     1201 ggggctcgcc gttgccgtcc ctgcccgtca cggtgaaccc gaccttgttg gcacccgccg
     1261 tcaccaggta ggcctgcatg gccaggcgtc ccgcgaaacc gtagccgtac gaatacctca
     1321 ggtgcatgaa ggtacgtccc gcatccagcg gcacggcgga cacgacgatg cggtagtcgt
     1381 gcgtgcccac cggtcccctg ccggcgtcga gccggctctc gaaatagccg gcggtggccg
     1441 cgacgggccg caacacgaag tccaggcgga acgcatgctc gaccggctgg tccgatctgc
     1501 ggccgatgcg caccagcaga ttcgccgcgg cgctgctccc gacggcatgg cagtacttgg
     1561 tgttgaaggg caggatcagg atgttgcacc agtccgcggg gtccttcagg gcaccgctga
     1621 cctcgcggaa cgaatggtcc agtacggcgt aaacgtcgcc cctgatcgtg tcgccggcct
     1681 gcgtggagtc gatgtgcagc gggcggtgaa agttgttgtt gcgcagctgc tcacgcagct
     1741 cgacgtacct ggtccgcagc tcgccgctgc ctgccagcgc gctcacgccc gtgaaaccca
     1801 ggacgaacac cagcccgagc cgcagcaggg cagcggccgc cggcgcgagc ctgcaccagg
     1861 gtatcttcat gcgtgatgca actccagcgc atgcgcgcgg tcaggggtct cgtcttcctc
     1921 cagtacccgc tccacgtcga actggatgcc gcccatgaaa agagaatgcg gctgcaccgc
     1981 gtagaggaag cggccacgct ggtccagctc caggcacatg cgcagcttcg ggttttcgac
     2041 ctgctcccac gccctgatca gccgcgtgcg cagaaggttg tcggtgcatt gcaggtaccg
     2101 gagcttgtgg tggttctgca cgaggttgac cgcgtcgagg tacgaggcgg cacttttgtt
     2161 gcgggtatgg aaattcggcg tgagatcgat gcccagcgtc agcacgcggc cttcgacctc
     2221 cagcgtgaag ttccggccca gcagcccgtc gcccgcctcg ccgtacttgt aggccaggtg
     2281 caaatgcgag ggtgtctttc gcaacgagta tttcatgacc ttcccgacat tgccgcggcg
     2341 gcaggcggcc ttcctgcagg ttgccttcga cgtggaaccc ggactgtagg acaacgcagc
     2401 atttgcctgt ggtccggccc gcccgcgcat ggcgccgggg tcggtgggcc cttctcctac
     2461 aggcactctc ccgtttgact gattgccccg tcaccaggca gaccgaacat gaccgggagt
     2521 gcacatgacg acgccttcgg cgcgcgtggc gcgaacagcg ggcgtgccgc cgcaagcaag
     2581 gcgcctcgcc aatccccatt catcgatcaa ggagatacac atggcagctg aagacaaaga
     2641 cctcaatcgc gacccgatca ccgatgagcc gggttcgcat cccgtcggca ccggcatcgg
     2701 tgccaccgga ggcgccgtgg caggcgcggc agccggaacc ctcggcggtc cggtgggcgt
     2761 tgctgtgggc ggcgtgatcg gcgccgtggt gggcggtctg gccggcaagg cggcagccga
     2821 agccgtcaat ccgacggccg aggaagccca ctggcgcgag cactacaccc gcgagccgta
     2881 ctacgaaccc ggccgcggtt tcgacgacta tgcgccggcc tatcgcctgg ggctgagcgg
     2941 ccgcacccgg tacgagggcg actgggacac cgcggagtcc aggctcgcat cggaatggga
     3001 atcggctcgt ggcggatcga gcctgagctg gccgcaggcc cagccggcca gccgcgcggc
     3061 ctgggacagg atagatgcca gccggccgat gcagaccgat tgggatcgca tgagcgagga
     3121 agaacgcgcg gccatgaccc acgggcgcac ggacagccgc atgagctccg gcgcgtccga
     3181 ccgcccgagc ggcgccgcgg ccgtgatggg cgagatgcag agcgccggcg acagcagcgg
     3241 cgatcgggac gatgtcatcg atgcgttgaa cgacctggtc gagacctgca aggacggcga
     3301 gtacggcttc aaggcctgcg ccgagcaggc caggcgggcg gacctgaagt cggtgttcat
     3361 gcagcgcgcg gacgattgcc gcggcgctgc gcaggaactt gccgagcaga tccgcggcct
     3421 cggcggcaag cccgaggacg gcggcagcgc cgtgggcgcg gtcgaacgcg gctgggtggc
     3481 cgtcagagcc aagctgtcaa cctacgagga cctcgcggtg ctggaagaat gcgagcgcgg
     3541 tgaggacaac gcagtggcgc gctatcgcaa agccttgagg aagccgctgc ccgccggcat
     3601 caagctgatc gtggaacgcc agttgcaagg ggcgcaacgc aaccacgacc agatcaagac
     3661 gctgcgcgac cagctccgcg cgtcggcctg acagcgcggc gtgccaatgg caatggccgg
     3721 ggccgcgacg gcgacggctg gctccaggcc ggcgttcggg ccggcctttt tttgctcggc
     3781 ccacgacaca aaggcggctt gtcctacacg ggccgggccg tgcctttttt atggtgaacg
     3841 cagaaaggcc gcgacgccat ggacgagcgt tcccccgacg ccctgaaaac ctaccgccgg
     3901 aagcgcgact tcgacatcac gccggagccg gctgccggcg gcgcggccaa cgagcaagag
     3961 cgggctttcg tcatccagaa gcactgggcc tcacgcctgc actacgacct gcgccttgaa
     4021 ctggaaggca ccatgaaaag ttgggccgtt cccaagggac cgagcttcga tccggccgac
     4081 aagcggatgg cggtccatgt ggaggaccac ccgatctcgt acaacacctt cgaaggcacg
     4141 ataccgccgg ggcaatacgg cgcgggcaag gtcatcgtct gggacaaggg cgtctggctt
     4201 gcgctggagg atccgcacaa gggctaccgc gagggcaagc tcaagttcga gttgcgcggc
     4261 cacaagttgc gcgggcagtg gacgctggta cgcatcaaga accgggccga cgaacggcag
     4321 gaaccctggt tgttgatcaa ggaaaaggac ggctacgccc gacccgcctt ggaattcagc
     4381 gttgtggacg aaatgcccga cagtgtgaaa cggctggcgg accggccgcc gcggggcgcc
     4441 acggcggcgc gggccggggc cgcgacggcc caaggcgcct cgggcccccc atcgggcgcg
     4501 cgcaaggcgc cgctgcccca ggcgctgtcg ccccagctgg ccacgctggg ggacaggccg
     4561 cccgccaatg ccggtcactg gatctacgag atcaagttcg acggctaccg catgctgacc
     4621 cgtgcgcagg acgggcaggt caagctggtc acccgcaacg gccacgactg gacccgcaaa
     4681 ctgccgcagc tcgcgcgcgc gctggagtcg atggcgttgc cggacggctg gtacgacgga
     4741 gaaatcatca tgccgggcga gcgcgtgccg gccgatttcc aggcgctgca gggcgccttc
     4801 gacaccgcgc gcacggcgca cgtcgtctat tacctgttcg acattcccta ttgcgccggg
     4861 tacgacctgc gcgacgtgcc gctgctggag cgccgcgccg tactgcagcg ggtggtccag
     4921 cgcaagccgc acgagaacgt ccggttcagc gcggtattcg acgcggcacc ccaggacgtg
     4981 ctggcctcgg cttgccgcct cggcctggag ggcgtgatcg cgaagcgcaa ggattcggcc
     5041 tacgtgccgc ggcgctcgtc cgactgggtc aagctcaagt gcgggctgcg ccaggagttc
     5101 gtcatcggcg gctacaccga cccgaaaggc tcgcgcaccg gcatcggctc gctgctgctg
     5161 ggcgtgcacg acaaaagcgg aagtctccgg tatgccggca acgtggggac cggtttcaat
     5221 gaagagaccc tgagcgacct gcgccggcgg ctcgacgcga tcgcggcggg ctccagcccg
     5281 ttcgcgtccg atgccgccac ccccaggaac gcacactggg tcaagcccga gctggtttgc
     5341 gaagtggctt tcggcgattg gacccgcgag ggacggatcc ggcattcggt gttccgtggc
     5401 gtgcgcaccg acaaggcacc cgctgccatc acccgggagg agccgcgcca cggcgcgccc
     5461 gtggacaggc cggccaaggg cgcgaagacg gcggtcagga cagccggcat gacccgcgac
     5521 aagcaagccg aggcgacggc tgtcgaactt ccctcgtcgc ttcgcgtcag caatccggac
     5581 cgggtgatcg acgccgcctc cggcaccacc aagatagcac tcgtgcgcta ttacgcgctg
     5641 gtggcgccgc tgatgatgga gcacctgaaa ggccggccgg tggcgctggt gcgcgcgccc
     5701 acgggcatcg gcggcgagat gtttttccag aaacacgccg agcgccacca gatgcccggc
     5761 gtggagcagc tcgacccggc gttctatccc ggccatccgc ccatgctgga ggtcgccacg
     5821 gcccgcggcc tgttgtcggc cgcccagatg aacgtgatcg agttccacac ctggaacgcg
     5881 gtgaaaagcg ccatcggcaa gcccgatcgg atgaccttcg acctcgaccc gggggaaggc
     5941 gtcaagtggc tgcaggtgca gcaggcggcg caactggttc gcggctttct cgccgagctg
     6001 ggcctcgcgg cattcctcaa gaccagcggc ggcaagggct tgcatgtggt cgtgccgatc
     6061 cgcaggcagc tcgactggga cagcgtcaag gatttctcgc aggcgatcgt gcggcacctg
     6121 gcgcgcgcca tcccccagcg cttcgtcgcc aagagcggcg cggccaaccg cgtcggcaag
     6181 atcttcatcg attacctgcg caatggcttt ggcgccacca cggtgtcggc ctggtccgcg
     6241 cgcgcgcggc cgggcctcgg gatttcggtg ccggtgcgct gggacgagct cgacggactg
     6301 cgcggcgggg atcactggac cgtcggcacc gtccataccc ggctcgacca gggcaacgcg
     6361 ccgtgggacg actacgaggc gggccgcaag gggcttgccg cggcgatgcg ggcgctgggt
     6421 ttcgaacgtt gaacgagggc cgccgcgggc gcgccggaag tcgcttcagc cgcgcgcgcc
     6481 ggcccaggga atcgacaaac ccacgcgttg acggccctcg cgcgtcagcc ccaccccctc
     6541 ggccgcagcg agcgcttcga ggtcggccca tgcaagcggg cgatacgggg tctccgtggc
     6601 gaaccccttg ttgccctcgg tcggctccaa agtgagcgtg agtccgagtg cggccgggct
     6661 tgcctgcgcc gacagcacga ggccggcgac cggcggggtc tcatcggtca tgtggatgat
     6721 ggctgcggtg aggacgttgc ggatcgccga gcggccgatt tcgccgggca cggatcccac
     6781 gtcgttgcgc agcgagtagc cccggaatgt caggctggtg gccagcagcc cgaggcattc
     6841 gcgcacgcct tcgtccaccg tgctcaccgc cccgtcttcc ggggccagcc agctgaccac
     6901 gtccaggcac gagttgagcg ccgcccgggc aaagccattg atcttctggg cgctgtcgtg
     6961 gacttcgccc aggtcgggct cgggcgcacg caggcgccgt tccatgactt cgtagatcat
     7021 cccgatcggc tgcaggttca ccaccaggtg atgccgcatc gagggcgcca gccgccgcag
     7081 caatgtgtag cgcgcggctt cagccagtcg ggcgttcggc aacgccgccg ccggctctcg
     7141 cgcagaatgg gctgtggggg tcattgccgg aagggtattt cacagcgtcg cggcttgtca
     7201 attggcgaga tggagcatcg gagcgccgga atgcggccgc aaatgcgggt tccagccggc
     7261 ggttgggttc cccagcgctg tcgagcagct ctcctcatct gttattcctt cgcgatgtcg
     7321 cgatgtggcc tcaggcgtga ggctcacact tcgcacatcg ggcgtgactt tggctgcttg
     7381 ccagttgctc aaggcgtggg gagcgggcca ttcaattcgc aggggtcctc gctgacggcg
     7441 gcaataacgg tgctcatatc cgccataagg gcgctaaaaa agcgccgtca tgctgatgct
     7501 tttcagatgc gttcgccgtc atcgtcgtga cggtagctga agaacaccag gtcacgttca
     7561 ggcatggcag tcgccggggt cagtggaaca aggcgtgggc ctcgcctggg aagtccgcgg
     7621 gcggctgctc gccagccttt cacgtcttct cgacgccttc ggcttccgcg cgcgaagaga
     7681 cgatgccgac gatgccgaac cccatgcgcc cggcgatcgg atagacgcgt gtacgcacgc
     7741 ggccccatga gcagttcctc ccagttacaa ggggcgcttt gtcacacgcc gggaaggctc
     7801 cccggctgta ccgtcccgca ggaaatcaat tgcggcggtt tgcgcctttc cggttatccg
     7861 gcgctgcgtc attgcactcg caggcggcag gtctgctctc gggcgcgact ggcgccgtgc
     7921 agaaatcaca ggaaccgagt gctcagcggt acatgttcaa tacgctggcc gggtgtactc
     7981 ttttattcga ttcggataca ttcggatcga ctcgcaggtg gcaccatcgc aatgggaggt
     8041 atacatgctt gagttcgtgc gcagccttcg tcgaaggcgc gatttctgga cgcggctgcc
     8101 cgctttcgcc ctggcttttc tggtcgccga aatcttctac aaattcaaaa gcttctcggt
     8161 tgaatgtgcg gccttcctgg tcacttggct catcatcgat gtgatcgtcg atcaattggc
     8221 caagcgcctg gagccaccca agggccgaag tcttcagtag cgagcgtggg ccaggaacct
     8281 ggacgcggtc gcgcatcgga caagcgtgta accatacggc gctgccgtgg cctcaccccg
     8341 ctgcccccgc gctgcgagtt cttgatcgac gtcacagaat ttcgcccgtt ccacccatca
     8401 ccgcgttgcc tcatgaacac tccagtgtgg cctaaggcgt ttccggcggg gatcgcggat
     8461 ccttccggtc aatttgcgct cgttcggcaa agtaacggtc gattgatggc agtgcggctt
     8521 catgacggga acgtggtgtg gcgccagcgc catgatgttg cgctgcagcc tttgttggtg
     8581 gattcccggc aagcggttgc tttgagtact tctcatgcgc aggtgttcgc tttcagtctg
     8641 cacactgctg cggcaggagc ggaatgggtc agcgctcgat tgccatggcc cgagtgggcc
     8701 accgatgcca atgcgcagtc ggttcagtcc gattgcgacg cgatatggct cggccaggac
     8761 atgggtttgc gttgggagct gcgtctgctc tatcgtggcg gcgcaaagcc ggggccggca
     8821 cggccaatgg ggcaaacagc caccgggaca tgccgggtcg acctgaggtc gggtgggatt
     8881 tcgtcgcttc caacttggcc cgagggcccc ggcctgctgg gctgggaacc atcacaggac
     8941 cccatggttg ttgcccagtg ccggatcaat gatcgcgaat atcggctgac cacgaggcac
     9001 gaaggctcca cggttattac ctccatttca gcgcgtgacg tccttaccgg gcatgtattt
     9061 tgggaagtgc ctctcgatga agtcgtcccc agggggccgc cgccactcag gcaatgatga
     9121 tccggcaagt tggcgctgga agcatagagg gttcgtcaaa ggaaaaaagg agaagaagat
     9181 ggcgattact tttcaggatg tagcccagaa agtgggggta agtgtcgagc tggtggagtt
     9241 gttgtgtgcc acgagaggca ccacgcttgc tggcttggtg aacattccgg aagggggcct
     9301 tcgccgggcg attcgccgtc tcacctatcc tgacctggct cgagcgcgcc acgagcactt
     9361 gctgaagatc gcacgggacg acgatggccg catccctccc aatgcagtcg atcaggcgct
     9421 caccgagctg atggccttgc gcaaacgcct gccacagcgg gcgatggtgg cggggctgcc
     9481 tgtggcaaag gaggtgacgt cgcttgagct tctgggagcc atcggccctg tcgtacccat
     9541 ggcagccgga ttggcgccca accgctggcg ctggctcggt cccggaaacg tgggcgggcg
     9601 cacgcgcgcg atcgctattc accccgtcga ttcgcagcgc atgcttgccg cgagcgcggg
     9661 agggggcgtg tggttcaccc aggatggcgg cgcgcgatgg gatcccgtcg acgatttcat
     9721 ggccaacctc gcggtgtgct gcctggccgt cgaccccaaa gaccccaacc aggtctatgc
     9781 gggaacggga gaaggcttcg gtaacggaga cgcgcttcgt ggggccggca tctttcgcat
     9841 cgtcggcggc aatcagtgga agcagctttc ggcaacccaa cactttccgg ctgtcaacag
     9901 aatcgccgtg tcggccgatg gaaaagtcat gctcgcggca acacctgaag gcttgcagcg
     9961 aagtgtggac gcgccgcgcg caagatggag gaaggtcctg gccgcccccc tggcagacgt
    10021 gaagtttcat ccggcgaatt ccaagctggc gattgcaggt ggtcttgagg atgggcaggc
    10081 atatttctcc aagaataagg gtcgaacctg gcagctcggc acacacgcca ccgcctggag
    10141 cgggcgggtg gagcttgctt atgcccgaaa gaaccccaac gttgtgtacg cttccgtgca
    10201 aggggacacg ggcgagcttt ggcgatcgga tgatggcggc aagacgtatc agcggcgccg
    10261 tgccctggac ccggacggcc aacccgcgca gtttctcggc gatcaaggct ggtatggaaa
    10321 tacggtctgg gccggcgacc cgggagacga aaacctcgtc ctagtcggag gcatcaattt
    10381 gtggcgctcc gttgacgggg gcgatacatt ggctgagatc agcacctggt gggacagccg
    10441 gtcggtgcac gccgatcacc attgcatagt ctctcacccg ggatacgatg gcaccatgaa
    10501 ccgtaccgtc ttttttggac atgacggcgg gatttccaag gccacggaca tccgcgtacc
    10561 gggctccgag gcacagccgc cctacgtgca gggatgggtc gagctgaaca acaactacgg
    10621 agtcacccag ttctatggcg gcgctgtgca ttccgcgacc gggcggatcg tcgcgggcac
    10681 tcaggacaat ggctctctct gtctcgatcc cggcgaagga accgatcaat ggcacagctg
    10741 gttcggaggc gacggtggtt ggtgcgcctc cgatccgacc gaccctcagg ttttctacgg
    10801 cgagtacgtg ttcctgaata tccatcggaa catggatgga tgtgcgacca gcgatcaaca
    10861 gggtgaccgg tacatcagcg ggcagttctg ggacgcgacc cgaagggact gggcgtggaa
    10921 gccggtgccg ttccagattt cggatgcacg aactcaggat gcgcttttca tagctccctt
    10981 tgtgctggat tcgcgccagc ccgaccgact gctcggcggc ggcctgtccc tgtgggagac
    11041 caaggatgca aaggcgccga atactccgac gagcggaccg cggtggagcc gcatcaagcc
    11101 cagtaccggc cgtttcatca gcgcgatcgc catctcacca gcgaactccg acgtggtttg
    11161 ggttgggcat caggacggcg ccgtgttcgt gaccacgacc ggcacgagcg gctctccggc
    11221 ctggcagcgt tgtcccgata ccggtcctgg cggcttgacg cccaagcgct attgcacgcg
    11281 aattgtcccg catcccaccg atctcaagac cgcctacgtg accttcgggg gattcaacca
    11341 cgataacgtg tggcagaccc aagactcggg ctctacatgg gcacctgtca gtggaaatct
    11401 acccgcggct ccggtttact cgcttgccat acatccgcgc cggctcaatt tcatctatgt
    11461 cggaacggaa gttggcattt tcggcagtga agattcaggg gtgtcctggt cgccgaccaa
    11521 cgaggggcct gcaaacgtat cggtcaatga attgttctgg aacggccaga ctctggtctg
    11581 tgcttctcac ggacgcggca tgttttcgat cgacctgaaa ggtgtttaag tcccgcccga
    11641 agagcgaggt tcgggtgagg tttgcgtggg ccacgcgctg gggcccgcgg ctatactgag
    11701 agcgcgactg aaccttggac atacctgcag ggagctccgt gaagcagcca gacacacacg
    11761 ccatcgagcg cactgtcgtc acgcagaaga acaatatctc gcgggatttc ctggacaccg
    11821 tgctcgacga cctcggcgag cgccccgacc tgctgccgac cgtgttccgg cgcgaagtgc
    11881 agctggcggc cgggctggcc agcaatgcgc gcagcgcgaa gaaggccgcg cctgaactga
    11941 tccgcgcggc gcgcagcgcg gctttgatgt tcgtgcacgc ctggcatcgc aacaagagca
    12001 ctttcatccc gctcgacgcc aggctggacg tcgaactgca gggcgaggtg gatccgagtt
    12061 tcgtcagcgc gccggcctgg atcgaggcga tgtggtgcgc gctggcttgc ggcgacgtct
    12121 ttgcgcagca gtggctggcc acggtcccac ccagccggct ggcgccggcc ggcgtccagc
    12181 atggccgcta cgcctttgcg ctgggcgaat tcctgcgctc gctggtcacg cgcgatggcc
    12241 gccacggcga atggctggtg caggccaccg aagcctccga cgacgacgac ggcgacccgc
    12301 tcctggaggc cacgcgcgat tgggccgacg gcatcgactt tcccgcgctg cgtgccgcct
    12361 tccatctgct cgacgaggac caggccggat tcgagcaggc gctcaccgag ctgcacgagc
    12421 aacacaagcg ctactggggc aatggggaaa accggctcgc cgtcgacggc ctgctgtcgc
    12481 tgcgcggctg cgcgctgctg cgcctgggcg cccaattgaa gatcaaggtg ggcttcgcca
    12541 gcggctacat gccacccgcc gtgtggcagg cgccggccgt gccgcacccc gtgcgctgcc
    12601 cgtattgcgt cgtaccgctg gcggccggcg cacgcgagtg cggcccctgt gggcgcgcta
    12661 tccaggacgc accgatcgaa ctggctgctg ccgcgacggc cggtccgcgc acgccctgca
    12721 agagctgcgc ctatccgctg cacaagctcg cggtgctgtg tccgcagtgc aggacggcgc
    12781 ggcgctagca cgccccttca gggcttcaag gtcttggcca ggttgaagtc cgccacctcc
    12841 accgccagca cctgactcct gggcttggcg tcgcgcgtca tctcgatcgg ggcgatcacc
    12901 agcttgtatt cgatttcgct gggcttgagc gccttcagca tggccgcgat cctgttcatt
    12961 tcgggcgtgc ccttttccat cgacgcgatg atgctttcga aataggcgcc ggtgccctgc
    13021 tgcaccacca ggccgccggc cttgcgcgtg cccagcgtgc ccgcaccgcc cttggcctcg
    13081 atcacgatca cctgcaccac gaccttcttg ccgcgcttgt tcttgccgta cttgacccag
    13141 acctggtcga agtcgcccgc gcgcgaaggc ttggtcgggt cgctggggta gagctgctcg
    13201 aagtccggat atttttcctt catgtaagcc ttggcgtgct cttcgccgag ttggcgcgag
    13261 gcctcgttca cgtcgcgcgc atgcccggcg gccgcttcct cgtcgccctt gcccagggcc
    13321 ttgtcgcggt cggcgcgcgc cttgtcgcgc ttggccagca ggtccttcat gcgctgcttg
    13381 tcggccttgg aaagcttcag gtcgtcaacc ggcacggctt gcctgttttc cttcagggcc
    13441 ctgatgtcgg gcaccgggtc gtccaccagc gtgggatcct tgaggcggcc ggtctcggga
    13501 ttcagccgca t
//
LOCUS       DASWIY010000097         4088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1914276, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000097 DASWIY010000000
VERSION     DASWIY010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914276"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VGA59_03790"
     CDS             <1..385
                     /locus_tag="VGA59_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX9718829.1"
                     /translation="MASGSGLPVRVHGAFVSDDEVHRVVTYLKQQGEPNYIEGLLEGG
                     TVDEDGDLLGGDGAASGEKDPMYDQAVEVVLKNRRASISLVQRHLKIGYNRAARLVED
                     MEKAGLVSAMSGSGQREILVPNRGE"
     gene            680..1312
                     /gene="lolA"
                     /locus_tag="VGA59_03795"
     CDS             680..1312
                     /gene="lolA"
                     /locus_tag="VGA59_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEX9718830.1"
                     /translation="MNRLIATLFFAACAVSAQASGLDSLENFVKSVKSGRAQFTQVVT
                     GPARDGQAARSKTSSGTFEFSRPNRFRFHYNKPFEQSIVADGQTLWLHDVDLNQVTAR
                     KQSQVLGSTPAALIAAAPDLATLRKDFNLEAAGEKDGLQWVQATPKVKEGQLHSVKVG
                     FRGNDLAALEILDSFGQRSVLTFTRMELNAAVSPELFQFKPPKGVDVVRQ"
     gene            complement(1344..2192)
                     /locus_tag="VGA59_03800"
     CDS             complement(1344..2192)
                     /locus_tag="VGA59_03800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9718831.1"
                     /translation="MANDYRIRPMKRDEIEIALDWAAAEGWNPGLNDASCFHAADPQG
                     FLLGLQGDEPVATISVVKYGADFGFLGLYIVKPGFRGRGLGLALWQAGMSSLAGRNVG
                     LDGVPAQQDNYRKSGFALAWRNIRQEGVGGGEAPADYRLVPLAAVPFGILHAYDQRFF
                     PADRSAFLRCWIGQPGSAAIGFAERERLKGYGVLRPCREGYKIGPLFADDPAIAESLF
                     LALKSRAGAGDKIYLDTPEANPQALALARRHSMKVVFETARMYTKGAPPLPMHQLYGV
                     TTFELG"
     gene            complement(2209..2580)
                     /locus_tag="VGA59_03805"
     CDS             complement(2209..2580)
                     /locus_tag="VGA59_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718832.1"
                     /translation="MDASLWSTFSALLGALVGGGISYALNRQLFANQLKLAQEQHRTE
                     FMAETTARHFLSHKGFTDRSFETLRQHLGGFDDDELRKILVRAGAMRIYRDDGSEWWR
                     LLSRMEEFIERKDLDRITREI"
     gene            2688..4007
                     /locus_tag="VGA59_03810"
     CDS             2688..4007
                     /locus_tag="VGA59_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX9718833.1"
                     /translation="MSKTVAIAHQPLAERLRPKTLGEVIGQQHLLGEGMPLRIAFESG
                     QPHSCILWGPPGTGKTTIARLMADAFDAQFITISAVLGGVKDIRDAVEQAQIAQGTAL
                     ENASGAAGQRTIVFVDEVHRFNKSQQDAFLPHVESGLFTFIGATTENPSFEVNSALLS
                     RAAVYVLQPLTEADLERIVAKARSIDAVPPVEKAALDRLVAYADGDARRLLNTLETLA
                     IAANKEKREEVTDEWVLKVLGERMRRYDKGGEQFYDTISALHKSVRGSDPDASLYWFM
                     RMLDGGADPRYMARRLIRMATEDIGLADPRALRMALDAAETYERLGTPEGELALAQCV
                     IYLAVAPKSNAVYKAFNEAKALIASDGTRPVPLHLRNAPTQLMKDLDYGKGYRYAHDE
                     EGGFAAGENYFPEGMTPPQFYRPVDRGLEMRIAEKLIELKKKNHQQR"
BASE COUNT          755 a         1328 c         1349 g          656 t
ORIGIN      
        1 catggccagc gggtcggggc tgccggtgcg tgtgcacggc gcgttcgtca gcgacgacga
       61 ggtgcatcgt gtggtcactt acctgaagca gcagggcgag cccaattaca tcgagggctt
      121 gctcgaaggc ggcacggtgg acgaggatgg cgacctgctg ggcggcgacg gcgcggcatc
      181 gggcgagaag gacccgatgt acgaccaggc cgtggaggtg gtcctgaaaa accgcagggc
      241 gagcatttcc ctggtccagc gtcacctgaa aattggttac aaccgcgcgg cgcggttggt
      301 ggaagatatg gagaaggcgg gcttggtcag cgccatgagc ggcagcggcc agcgcgaaat
      361 cctggtgccg aaccggggcg aataacgacc ggtaacgaac ttcgggctgc cagcccaacc
      421 aaaccactgt catgccggac tcgatccggc atccatggat tgcgggtcaa gcccgcaatg
      481 acaaacgggg ctgttatccc gggcttgacc cgggatccat gactcctgaa ttcatggatt
      541 gcgggtcgag tccgcaatga caaacggggc tgtcatcccg ggcttgaccc gggatccatg
      601 actcctgaat tcatggattg cgggtcgagc ccgcaatgac aatactcatg gcgcaatgac
      661 acgatcaaca gggagtccca tgaatagact gattgccaca cttttcttcg ccgcctgcgc
      721 cgtgtcggcc caggcctccg gcctcgacag cctggaaaac ttcgtcaagt ccgtcaagag
      781 cgggcgggcg caattcacgc aggtggtgac cgggccggcc cgggacggac aggccgcgcg
      841 cagcaagacc tcgtcgggca cgttcgagtt ctcgcggccc aaccgcttcc gcttccacta
      901 caacaagccg ttcgagcagt ccatcgtcgc cgacggccag accctctggc tgcacgacgt
      961 ggacctgaac caggtcaccg cgcgcaagca gtcgcaggtg ctggggtcca cgccggcggc
     1021 cctgatcgcc gccgccccgg acctcgccac gttgcgcaag gatttcaacc tggaagcggc
     1081 cggcgagaag gacgggctgc agtgggtgca agccacgccc aaggtcaagg aaggccagct
     1141 gcacagcgtg aaggtgggat ttcgcggcaa tgacctcgcc gcgctggaaa tcctggacag
     1201 cttcggccag cgctcggtgc tgacgttcac caggatggag ctcaacgcgg ccgtgtcgcc
     1261 cgagctgttc cagttcaagc cgcccaaggg cgtggacgtg gtgcgccaat agcgcgtgcc
     1321 gcccgcgcgt tcccgcgttt ctctcaaccc agctcgaagg tggtcacgcc atagagctgg
     1381 tgcatcggca gcggcggcgc ccccttcgtg tacatgcgtg ctgtctcgaa cacgaccttc
     1441 atcgagtggc ggcgcgccag ggccagcgcc tgcgggttgg cttcgggcgt atcgaggtag
     1501 atcttgtcgc ccgcgccggc acgtgacttg agcgccagga acaggctttc ggcgatggcg
     1561 ggatcgtcgg caaagagcgg cccgatcttg tacccctcgc gacacggccg cagcaccccg
     1621 tagcccttga gccgttccct ttccgcaaag ccgatggccg cgctgccggg ctgcccgatc
     1681 cagcacctca gaaaagcgct tcgatcggcg gggaagaagc gctggtcgta cgcgtgcaag
     1741 attccaaagg gcacggccgc gagcggcaca aggcggtaat cggcgggcgc ctcgccgccg
     1801 cccacgcctt cctgccggat gttgcgccag gccagcgcaa agcccgactt gcggtagttg
     1861 tcctgctgcg ccggaacgcc gtcgagtccc acgttgcggc cggccagcga ggacatgccg
     1921 gcctgccaga gcgccaagcc caggccgcgg ccacgaaagc cgggtttgac aatgtagagg
     1981 ccgagaaagc cgaagtcggc gccgtacttc acgaccgaaa tggtggccac tggctcgtcg
     2041 ccctgcaagc ccagcaggaa accctgcgga tccgccgcgt ggaagcagga agcatcgttc
     2101 aatcccggat tccatccttc ggcggccgcc cagtccagcg cgatttcgat ttcatcgcgt
     2161 ttcatggggc ggatgcggta gtcgtttgcc atggcgacct ccttgcggct agatctcgcg
     2221 cgtgatcctg tccaagtcct tgcgctcgat gaattcttcc atccgcgaga gcagccgcca
     2281 ccattcgctg ccgtcgtcgc ggtagatgcg catcgcaccg gcgcgcacca ggatcttgcg
     2341 cagctcgtcg tcgtcgaagc cgcccaggtg ctggcgcagc gtttcgaacg agcggtcggt
     2401 gaaacccttg tggctgagga aatgccgtgc ggttgtttcc gccatgaact cggtgcggtg
     2461 ctgctcctgc gccagcttca gctggttggc gaacagctgg cggttgagtg cgtagctgat
     2521 gccgccgccc acgagcgcgc cgagcagcgc cgagaaggtc gaccagagcg aagcgtccat
     2581 ggtggccgca ctatacaagg ccttgtcatg ccgggctgac ctggcatcca cgaatgcatg
     2641 gattgcgggg caagcccgca atgaccgcag gtttctatac tgtgccggtg agcaagaccg
     2701 tcgccatcgc gcaccagccc ctggccgaac gcctgcgccc caagaccctg ggcgaggtga
     2761 tcggccagca gcacctgctg ggggagggca tgccgctgcg catcgccttc gagtcgggcc
     2821 agccgcacag ctgcatcctc tggggcccgc cgggaaccgg caagaccacc atcgcgcggt
     2881 tgatggccga cgcgttcgac gcgcagttca tcaccatcag cgccgtgctc ggcggtgtca
     2941 aggacatccg cgacgcggtg gaacaggccc agatcgccca gggcacggcg ctggaaaacg
     3001 cctccggcgc ggcgggacag cgcaccatcg tgtttgtcga tgaagtgcat cgcttcaaca
     3061 agagccagca ggacgccttc ctgccgcatg tggaatcggg cctgttcacg ttcataggcg
     3121 cgaccacgga gaacccctcg ttcgaagtca attccgccct gctgtcgcgc gcggctgtct
     3181 atgtgctgca gccgctcacc gaggccgacc tggaacggat cgtggccaag gcgcgatcga
     3241 tcgacgcggt accgcccgtg gaaaaggccg cgctggaccg gctcgtcgcc tatgccgacg
     3301 gcgacgcgcg gcggctgctc aacacgctcg agacattggc catcgccgcg aacaaggaaa
     3361 agcgcgagga ggtcacggac gagtgggtgc tcaaggtgct gggcgagcgc atgcggcgct
     3421 acgacaaggg cggcgagcag ttctacgaca cgatcagcgc gctgcacaag tcggtgcgcg
     3481 gctccgaccc ggatgcttcg ctctactggt tcatgcgcat gctcgacggt ggcgccgatc
     3541 cgcgctatat ggcacggcgc ctgatccgca tggccaccga ggacatcggc ctggccgacc
     3601 cgcgcgcctt gcgcatggcg ctcgacgcgg ccgagacgta cgaacggctg ggcacccccg
     3661 agggtgagct ggcgctggcg cagtgcgtga tctaccttgc ggtggcgccc aagtccaacg
     3721 cggtctacaa ggccttcaac gaggccaagg cgctgatcgc cagcgacggc acccggccgg
     3781 tgcccctgca cctgcgcaac gcgcccacgc agctgatgaa ggacctggac tatggcaagg
     3841 gctaccgcta cgcgcacgac gaagagggcg gcttcgcggc cggcgagaat tattttccgg
     3901 aagggatgac gccgccgcag ttctatcggc cggtggaccg tgggctggaa atgcgcatcg
     3961 ccgaaaagtt gatcgagctc aagaagaaaa accatcagca acgctgatga ccccggtaaa
     4021 acgccattcg gacggcgccc ggcagggtaa acccccgcca tcggcacaat attgctgtca
     4081 ggtcgctt
//
LOCUS       DASWIY010000098         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1538032, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000098 DASWIY010000000
VERSION     DASWIY010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538032"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /gene="tgt"
                     /locus_tag="VGA59_03815"
     CDS             complement(<1..948)
                     /gene="tgt"
                     /locus_tag="VGA59_03815"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX9718834.1"
                     /translation="MLHFELLKTEGRARRGRLTLNHGVVETPIFMPVGTYGTVKGVTP
                     RSLEEIGAQIILGNTFHLWMRPGLDVVKQFGGLHRFECWSRPILTDSGGFQVWSLGEM
                     RKIGEEGVKFASPVNGDKLFLTPEGSMQIQTLLASDIVMQFDECTPYDTRGHVTTEAE
                     ARFSMELSRRWAQRCQAEFARLANPNALFGIVQGGMYEHLREESVAALVEMDFPGYGI
                     GGVSVGEPKEEMLRIMAHTPHRLPAGKPRYLMGVGTPEDLVEGVAQGVDMFDCVMPTR
                     NARNGHLFTRFGDLKIRNARHRADERPLDETCSCYTCKGS"
     gene            complement(978..2060)
                     /gene="queA"
                     /locus_tag="VGA59_03820"
     CDS             complement(978..2060)
                     /gene="queA"
                     /locus_tag="VGA59_03820"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX9718835.1"
                     /translation="MRTFTLGDFDFALPPELIAQHPAAERSASRLLDGTGPEPMDRIF
                     RELPALLEAGDLLVFNDTKVVKARLFGEKPTGGKVELLIERVLADREVVAHMKVSKKP
                     PAGTVLRMQGGFTATLNGRWPDDEGPLFRFTLSGEPHGLMERHGHVPLPPYIEHADTA
                     EDVRRYQTVFARHPGAVAAPTAALHFDEALLAQLDARGVRRASVTLHVGAGTFQPVKT
                     ENLSEHRMHSEWYAMPPATQQAIADVRERGGRVVAVGTTTVRTLESWAQTGQASGDTS
                     IFITPGFQFRLVDRLVTNFHLPRSTLLMLVSAFAGYDSLMNLYRHAVAQRYRFFSYGD
                     AMLLEKFAHPRSDLRLTSGPAKPDPR"
     gene            2176..>3947
                     /locus_tag="VGA59_03825"
     CDS             2176..>3947
                     /locus_tag="VGA59_03825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX9718836.1"
                     /translation="MPDTKSPSEPRIPPGRPTVSDSPARADAGSRRPAGPQAALIKLG
                     LERDIDLALHLPLRYEDETRLVKLCDAREGDTAQIEATVTACEIVYRPRRQLMATVDD
                     GSDTCVLRFFSFYPSQQKALAVGTRLRVRGELKGGFLGWQMVHPSFKPAGGELPGALT
                     PVYSTVAALPQAYLRKAVLGGLARADLLDTFPPGVTQALRPAAWDLREALSFLHQPAP
                     DVPLGTLEDRSHPAWQRLKAEELLAQQLSQLESKRQRATLRSPAMAARPQGLHEQLLA
                     ALPFRLTAAQRRVGEEIARDLARSVPMHRLLQGDVGSGKTVVAALAAAIAIDAGWQCA
                     LMAPTEILAEQHFRKLIGWLEPLLAPQGLRVAWLTGSQKKRERTEMLSLVAGGEAALV
                     VGTHAVIQDQVQFRNLGLAIIDEQHRFGVAQRLALRAKMDHAAEPFPSRGRVGEGGST
                     DSPGEGRAQREPHLLMMSATPIPRTLAMSYYADLDVSTIDELPPGRTPVVTKLIADSR
                     REEVAERIRAQVARGRQVYWVCPLIEESEALDLTNATATHEALSAALPGVMVGLLHSR
                     MPAPEKKAVMSLFTGGQMGVLVATT"
BASE COUNT          627 a         1330 c         1356 g          634 t
ORIGIN      
        1 gctgcccttg caggtgtagc agctgcaggt ttcatccagc gggcgctcgt cggcgcggtg
       61 cctggcgttg cggatcttca ggtcgccgaa gcgcgtgaac aggtggccgt tgcgcgcgtt
      121 gcgcgtcggc attacgcagt cgaacatgtc cacgccctgc gccacgccct cgaccaggtc
      181 ctcgggcgtt ccgacgccca tcaggtagcg gggcttgccg gccggcaatc gatgcggcgt
      241 gtgggccatg atgcgcagca tctcctcctt gggttccccg acgctgacgc cgccgatccc
      301 gtaaccggga aagtccattt cgaccagcgc cgccaccgac tcctcgcgca ggtgctcgta
      361 catgccgcct tgcacgatgc cgaacaaggc gttgggattg gccagccgcg cgaactcggc
      421 ctggcagcgc tgcgcccagc gccggctcaa ctccatggaa aagcgggctt cggcttcggt
      481 ggtgacatgg cccctggtgt cgtagggcgt gcactcgtcg aactgcatca cgatgtcgct
      541 ggccagcagc gtctggatct gcatcgaacc ctcgggcgtg aggaacagct tgtcgccgtt
      601 cacgggcgac gcgaacttca cgccttcctc gccgatcttg cgcatctcgc ccaggctcca
      661 gacctggaag ccgccggagt cggtcaggat cggccggctc cagcactcga accggtgcag
      721 cccgccgaac tgcttcacca catccagccc cggccgcatc cacagatgga aagtgttgcc
      781 caggatgatc tgcgcgccga tctcttccag cgaccgcggg gtgacgcctt tcaccgtgcc
      841 gtaggtgccc accggcatga agatcggtgt ctcgaccacg ccgtggttca gggtcaggcg
      901 gccgcggcgt gcgcggcctt cggtcttgag cagttcgaag tgcagcatcg gagtcgaagc
      961 gattgtcttt gcgggggtca ccgcggatcc ggctttgccg ggccgctggt gagccggagg
     1021 tcgcttcggg ggtgggcaaa tttctcgagc agcatcgcgt caccgtagct gaaaaagcga
     1081 tagcgttgcg ccacggcgtg gcggtacagg ttcatgaggg aatcgtagcc cgcaaacgcg
     1141 ctcaccagca tcagcaatgt ggagcggggc aggtggaagt tggtgaccag gcggtccacg
     1201 aggcggaact ggaagccggg cgtgatgaag atgctggtgt cgccgctcgc ctggccggtc
     1261 tgggcccagg actcaagggt gcgaaccgtg gtggtgccga cggccaccac gcgaccgcct
     1321 cgttcgcgca catcggcaat cgcctgctgc gtagcgggcg gcatcgcgta ccactcgctg
     1381 tgcattctgt gctcggacag gttttcggtc ttgaccggct ggaacgtgcc ggcacccaca
     1441 tgcagggtga cgctggcgcg ccggacgcct cgcgcgtcga gttgcgcgag cagtgcctcg
     1501 tcgaagtgca gtgccgcggt cggcgctgcc accgcgccgg gatgcctggc gaaaacggtc
     1561 tggtagcgcc ggacatcttc cgccgtgtcg gcatgctcga tgtagggcgg cagcggtaca
     1621 tggccgtgcc gctccatcaa gccgtgcggc tcgccgctga gcgtgaagcg gaacagcggg
     1681 ccctcgtcgt cgggccagcg gccgttcaag gtggcggtaa agccgccctg catgcgcagc
     1741 acggttccgg ccggtggttt cttgctcacc ttcatgtgcg cgacgacctc ccggtccgcc
     1801 agcacccgtt cgatcagcag ttccactttg ccgccggtcg gcttctcgcc gaacaggcgg
     1861 gctttcacga ccttcgtgtc gttgaacacc agcaggtcgc cggcttcgag cagcgccggc
     1921 agctcgcgaa aaatacgatc catcggctcc ggccccgtcc catcgagcag gcgcgaagcg
     1981 ctgcgctcgg cggcgggatg ctgggcgatc agttcgggcg gaagagcgaa atcgaaatcg
     2041 ccgagggtga aggtacgcat gcttgagggc gtcagcgccg cgcgccgccg cgagggccag
     2101 gatgggaatg catcgcgacc gcgatgcacg agacccgttc caggttgggc gaagagagac
     2161 aggcagaatt gtcccatgcc cgacaccaag tcgccgtccg agcctcggat accaccgggc
     2221 cgccccacgg tttccgattc ccctgccagg gcggacgccg gatcgcggcg gcccgcgggc
     2281 ccccaggccg ccctgatcaa gctcggtctc gagcgcgata tcgacctggc gctgcatctg
     2341 cccctgcgct acgaggacga aacgcggctg gtgaagctgt gcgacgcccg cgaaggcgac
     2401 acggcgcaga tcgaagccac ggtgaccgcc tgcgagatcg tctaccggcc gcgccggcaa
     2461 ctgatggcca ctgtggacga cggcagcgac acctgcgtgc tgcgcttttt cagcttctac
     2521 ccttcccagc aaaaagcctt ggccgtcggc acccgcttgc gcgttcgcgg cgagctcaag
     2581 ggaggctttc tcggctggca gatggtccat ccttcgttca agcccgcggg cggagagctg
     2641 ccaggcgcgc tgactccggt gtattccacc gtcgcggcgc tgccccaggc ctacctgcgc
     2701 aaggcggtgc tgggagggtt ggcgcgggcc gatctcctgg acacctttcc tcccggtgtc
     2761 acccaagccc tgcggcccgc ggcgtgggac ctgcgcgagg ccctgtcgtt cctgcaccag
     2821 ccggcgccgg acgtgccgtt gggcacactg gaagaccgct cccatcccgc ctggcaacgc
     2881 ctgaaagccg aagagctgct ggcgcagcag ctgtcgcagc tggaaagcaa gcgccagcgc
     2941 gcgacactgc gctcacccgc catggcggcg cggccacagg ggctgcacga gcaactgctg
     3001 gcggcgctgc cgttccggct cacggcggcc cagcggcgcg tgggcgagga aattgcgcgg
     3061 gacctcgcgc gcagcgtccc catgcaccgc ctcctgcaag gcgatgtcgg ttccggcaag
     3121 accgtggtgg ctgcgctcgc ggcggccatc gccatcgacg ccggctggca atgtgcgctg
     3181 atggcgccca ccgaaatcct ggcggagcag catttccgca agctgatcgg ctggctggaa
     3241 ccgctgttgg cgccgcaggg gctgcgtgtg gcctggctca cgggcagcca gaaaaagagg
     3301 gaacgcaccg aaatgctgtc cctggtcgcc ggcggcgagg ccgcgctggt ggtgggcacc
     3361 cacgccgtga tccaggacca ggtgcagttc aggaatctcg ggctggccat catcgacgag
     3421 cagcaccgct tcggggtcgc ccagaggctc gcgttgcggg ccaagatgga ccatgctgcg
     3481 gaacccttcc cctcgagagg aagggtcggg gaggggggga gcacggactc acccggtgag
     3541 ggtcgcgcgc agcgcgaacc tcacctgctg atgatgagcg ccacgcccat cccgcgcacg
     3601 ctcgcgatga gctattacgc cgacctcgac gtctccacca tcgacgagct gccgccgggc
     3661 cgcacgccgg tcgtgaccaa actgatcgcg gacagccggc gcgaggaggt ggcggagcgt
     3721 atccgcgcgc aggtggcccg gggccgccag gtgtactggg tctgcccgct gatcgaggaa
     3781 agcgaagcgc tggacctgac caatgcgacg gccacgcacg aggcgctgag cgccgccctg
     3841 ccaggcgtca tggtcggcct gctgcattcg cgcatgccgg cgccggaaaa aaaggccgtt
     3901 atgtcgctct tcaccggcgg ccagatgggc gtgctcgtcg ccaccac
//
LOCUS       DASWIY010000099         4129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1166110, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000099 DASWIY010000000
VERSION     DASWIY010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166110"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VGA59_03830"
     CDS             <1..633
                     /locus_tag="VGA59_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX9718837.1"
                     /translation="RILDIGVVRLKSDTEVTAFEPVFESAARLGALHAVVNGDEPDHA
                     ILAILLARLCEVARPYALTMNLEPTPWTGVRTLAQAVKVVQAADQSEARVLVDTIHVD
                     RSGGTPSDISAVPSALIAYAQVCDAYGPRPTAFDTMIFQARNERRFPGEGNLDLRSML
                     RALPTGIPWSLEAPTRTLARTLSALERAKRGRAAVESLLASGECSHCDVK"
     gene            779..2551
                     /locus_tag="VGA59_03835"
     CDS             779..2551
                     /locus_tag="VGA59_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9718838.1"
                     /translation="MKDLISHQLVKYLEDRGVQHIFGLCGHTNIAVLAALEKSSIKFV
                     NTRHEQVAAHAADGYARARRQTAVVLSHLGPGLTNAATGIANASLDSIPLVVIAGDVP
                     SHYYGKHPHQEVNLHSDASQSEIYRPFVKRVWRVDRPDLFPEIIEKAFQLAETGRPGP
                     VLVSVPMDIFSKEVDVALFERLKLHTKKLHKPSIDDETATRIVQMLAAAKSPLIHVGG
                     GVVLADATAELAEFVTHMGVPVSHSLMGKGVLPDDHPMTLGMTGFWGTKFINDKCRNA
                     DVVFGLGTRFAEADCSSWESEYTFSFPPSKLIHIDIDPSEIGRNYPVAIGAVADLKQA
                     LTVLNRVAKKLYPKGRSNPALEQQIAANREQFQAGNRAHERSDAFPLRPERILADVRA
                     VLPRDAFITTDVGWNKNGVGQQFPVYEPGSILTPGGFATMGFGAPAAIGAKIAFPDRV
                     VVALVGDGGFGQNPAMLATAVQEDVAVVWIIMNNCAFGTIAGLEKAHFGTTFGTEFHK
                     DGKPYLVDYAAIARAYGAEGVQITSAGEFKPALERAIASKRPFVIDVAMQNAPVPTDG
                     HWNIMDIYSPGKKVHHVDTSAPRT"
     gene            2605..4035
                     /locus_tag="VGA59_03840"
     CDS             2605..4035
                     /locus_tag="VGA59_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9718839.1"
                     /translation="MSAPELVKTAINEEQKAELDAAFERARKALAIIETYDQARVDRL
                     CQAVAWAVSNKKTFTDLVTMGIAESGLGDAESRMNKRMKIRGVLRDALRQKSVGIIEE
                     LPEKGIAKYGKPAGVIACIVPTTNPDLTPAGNAIYAIKARDVVIFSPHPRAKKTSFET
                     VRLMREALEREGAPPDILQCLTKVNIPMSQALMARADLVIATGGQPMVRAAYSSGTPA
                     YGVGAGNATMIIDETANIEEAARNTRLSKTSDFGSGCSADGNLVIQEGIFDAMLAQLV
                     KEGGYLASEPEKALLRKAMWDDEGHRLPDTVAIAPQKLAAAAGFQIPEDRKFIIVRGD
                     GIGKAHPFSGEKLTTLLAVYKYGTFDQALDMMRSIYEVGGKGHSCGIYSFDQDHVHRL
                     AMAAPVSRIMVRQPQSKANAGAFNNGMPMTSSLGCGTWGGNVVSENVHLKHYMNTTWV
                     SLPIKEDRPSDEELFGSFYDARLEAA"
BASE COUNT          790 a         1351 c         1316 g          672 t
ORIGIN      
        1 cgtatcctcg atatcggcgt ggtgcggcta aaaagcgaca ccgaggtgac cgccttcgag
       61 cccgtcttcg aatccgccgc gcggttaggc gctcttcacg ccgtagtcaa cggtgacgaa
      121 ccggatcacg ccatcctcgc gatcctgctt gcccggttgt gcgaggtggc acggccgtat
      181 gcgctgacca tgaatctgga acccactccg tggacaggtg tcaggacact ggcgcaggcc
      241 gtgaaagtgg tgcaggctgc ggatcagtcc gaggctcgcg tgctcgtcga tacgatccat
      301 gtggatcgca gcggcggcac gccttcggac atcagtgcag ttccctcggc attgatcgct
      361 tatgcgcaag tgtgcgatgc gtacggcccg cggccgacgg ccttcgatac gatgatcttc
      421 caggcgcgca acgagcgccg cttccccggt gaaggcaacc tggatctgcg cagcatgctg
      481 cgcgcattgc cgactgggat cccatggagc ctggaagcgc ccactcgaac actggcgcgg
      541 accctctcgg cgctcgagcg cgcgaagcgc ggccgggccg ccgtggagtc gctgctcgcc
      601 tcaggcgaat gctcccattg cgacgtgaaa tagtaattat tgcgctcatg gcatggacga
      661 catggagaag cgcgcgtatc ttcatgccag atatcggtgg atggcaatcc gcgcccggcg
      721 tcgcttgaaa gttaatcgaa tgtactcaaa ggcattcgcg ctcatgtttg gagaccccat
      781 gaaagacctg atttcccacc agctggtcaa gtacctcgag gaccgaggcg tacaacacat
      841 cttcggcctt tgcggccaca cgaacatcgc ggtgcttgcc gcgcttgaaa aaagctcgat
      901 caagttcgtc aatacccgcc acgaacaggt tgccgcgcat gcggctgatg gctacgcgcg
      961 cgccaggcgg cagaccgccg tcgtgctgag ccacctgggc ccgggcctca ccaacgcggc
     1021 cactggcatc gccaacgcct cgctggattc cattcccctg gtggtgatcg cgggcgacgt
     1081 gccgagccac tattacggca agcatccgca ccaggaggtc aacctgcatt ccgatgcgtc
     1141 gcaatcggag atctaccggc ctttcgtgaa gcgcgtctgg cgcgtagacc ggcccgacct
     1201 gttccccgag atcatcgaga aagccttcca gctggccgag accggccggc ccggcccggt
     1261 gctggtgtcg gtgccgatgg acatcttctc caaggaagtg gacgttgcgt tgttcgaacg
     1321 cctgaagctg cacacgaaga agctgcacaa gccgtccatt gacgatgaaa cggccacgcg
     1381 catcgtgcag atgctggcgg cggcgaagag ccccttgatc catgtcgggg gtggcgtcgt
     1441 cctcgcggat gccaccgctg aactcgccga attcgtcacc cacatgggcg tgcccgtgtc
     1501 gcacagcctg atgggcaagg gtgtgctgcc ggacgatcat cccatgacgc tgggcatgac
     1561 gggcttctgg gggacgaaat tcatcaacga caagtgccgc aacgcggacg tggtcttcgg
     1621 cctgggaacg cgttttgcgg aagccgactg cagttcctgg gaatcggaat acaccttcag
     1681 ctttccgccc agcaagctga tccacatcga catcgacccg agtgaaatcg gccgcaacta
     1741 tccggtcgcg ataggcgccg tggccgacct gaagcaggcc ctcacggtgc tcaaccgggt
     1801 ggcgaagaag ctctacccga agggccgcag caacccggcg ctcgagcagc agatcgccgc
     1861 caaccgggag cagttccagg ccggcaaccg cgcccacgag cgcagcgacg ccttcccgtt
     1921 gcgacccgag cgcatcctgg ccgacgtgcg cgcggtgctg ccgcgcgacg cgttcatcac
     1981 caccgacgtc ggctggaaca agaacggcgt cggccagcag ttcccggtgt acgagccggg
     2041 cagcatcctg acccccggcg ggtttgccac catgggcttc ggtgcccccg cggccatcgg
     2101 cgcgaagatc gcatttcccg accgggtcgt ggtggccctg gtgggtgacg gcggcttcgg
     2161 ccaaaacccc gcgatgctcg ccacggcggt gcaggaagac gttgccgtgg tgtggatcat
     2221 catgaacaac tgcgctttcg gcaccatcgc tggcctggag aaggcgcact tcggcaccac
     2281 cttcggcacc gaattccaca aggacggcaa gccctacctg gtggactacg cggccatcgc
     2341 tcgtgcctac ggcgccgagg gcgtgcagat cacctcggcc ggcgagttca aaccggcgct
     2401 ggagcgcgcg atcgcgtcca agcggccctt cgtcatcgac gtcgcgatgc aaaacgcccc
     2461 cgtgcccacc gacgggcact ggaacatcat ggacatctat tcgcccggca agaaagtcca
     2521 tcacgtggac accagcgcgc cgcgcacttg aagcaaccgg ccgcctggcc gccgtacgaa
     2581 ccctacccct tttggagaat agacatgagt gcccctgaac tggtgaagac agccatcaac
     2641 gaagaacaga aagccgagct cgatgccgcc ttcgagcgcg cgcgcaaggc cctggccatc
     2701 atcgagacct acgaccaagc ccgggtggac cggctgtgcc aggccgtggc ctgggcggtc
     2761 tccaacaaga agacgttcac ggatctggtc acgatgggga ttgccgagag cggcctgggc
     2821 gacgcggaaa gccgcatgaa caagcgcatg aagatccgag gcgtgctgcg cgatgcgctg
     2881 cgccagaaga gcgtgggcat catcgaggag ctaccggaaa agggcatcgc caagtacggc
     2941 aagcccgcgg gcgtcattgc ctgcatcgtg cccaccacca accccgacct gacccctgcc
     3001 ggcaacgcga tctatgcgat caaggcgcgc gatgtcgtca tcttctcgcc ccatccgcgg
     3061 gccaagaaga cctcgttcga gacggtgcgc ctgatgcgcg aggcgctcga gcgcgagggt
     3121 gcgccgccgg acatcctgca atgcctgacc aaggtgaaca tcccgatgtc gcaggcgctg
     3181 atggcgcgtg ccgacctggt gatcgcaacc ggcgggcagc cgatggtgcg cgccgcctac
     3241 agctcgggca cgccggccta cggtgtgggc gcgggcaacg ccacgatgat catcgacgag
     3301 accgccaaca tcgaggaagc ggcgcgcaac acccgcctga gcaagacgtc cgatttcggc
     3361 tcgggctgct cggccgacgg caacctcgtc atccaggagg gcatcttcga tgcgatgctc
     3421 gcgcagctcg tcaaggaggg cggctacctg gccagcgagc ccgagaaggc cttgttgcga
     3481 aaggcgatgt gggatgacga aggccatcgc ctgcccgaca cggtcgccat tgcgccgcaa
     3541 aagctcgcgg ccgccgccgg gttccagatt ccagaggatc gcaagttcat catcgttcgc
     3601 ggcgacggca tcggcaaggc gcatcccttt tcgggcgaga agctcacgac gctgctggcg
     3661 gtgtacaagt acggcacctt cgaccaggcg ctggacatga tgcgctcgat ctacgaggtg
     3721 ggcggcaagg gccactcctg cgggatctac tcgttcgacc aggaccatgt ccaccggttg
     3781 gcgatggccg cgccggtcag ccgcatcatg gtgcgccagc cgcaatcgaa agcgaacgcc
     3841 ggcgcattca acaacggcat gccaatgacg tcgagcctgg gctgcggcac ctggggcggg
     3901 aatgtggtgt cggaaaacgt tcacctgaag cactacatga acaccacctg ggtgtcgctg
     3961 ccgatcaagg aagaccgccc atccgacgag gagctgttcg gctcgttcta cgacgcgagg
     4021 ctggaggccg cctgaaccgg cccggcccca tgagcgcagc gctggcgcgt gtccaacagt
     4081 gggagttgcc caggctgggt gccttctgga tggtcgttgc cggcctgct
//
LOCUS       DASWIY010000100        10563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_426324, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000100 DASWIY010000000
VERSION     DASWIY010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10563
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426324"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /gene="minE"
                     /locus_tag="VGA59_03845"
     CDS             complement(<1..248)
                     /gene="minE"
                     /locus_tag="VGA59_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEX9718840.1"
                     /translation="MGSFLSFLLGEKKKTASVAKERLQIILAHERSGRNAGQPDYLPA
                     LQRELVAVISKYIKIGPQDIKVNVDRQDNLEVLEVKIE"
     gene            complement(252..1067)
                     /gene="minD"
                     /locus_tag="VGA59_03850"
     CDS             complement(252..1067)
                     /gene="minD"
                     /locus_tag="VGA59_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEX9718841.1"
                     /translation="MARIVVVTSGKGGVGKTTTSASFAAGMAMAGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEANLHQALIKDKQCDNLSVLAASQTRDKEALTKDGV
                     EKVLNDLGGMGFDYIVCDSPAGIETGALMAMHFADEALVVTNPEVSSVRDSDRILGML
                     ASKTRRAIEGKEPVKEHLLITRYNPNRVDQGQMLSLEDIKDILRIKLIGVIPESETVL
                     QASNQGLPAVHMKGSEVSEAYKDVVDRFLGKDTPMRFVDPQKGGFLKRLFGGK"
     gene            complement(1088..1855)
                     /gene="minC"
                     /locus_tag="VGA59_03855"
     CDS             complement(1088..1855)
                     /gene="minC"
                     /locus_tag="VGA59_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEX9718842.1"
                     /translation="MTVALAGRAPATFEIKSANLPLVALLLKSSDLRALTAELAARFG
                     DIPDFFDDDPLVIDLSPLQERGDTALEFEALLALLRSYRMLPVAVRGGSAAQMAAALQ
                     AGLAAAPEAVAAAPEPQRTRKAPTAPAPELVPVSALVVDKPLRSGQQIYARGRDLVML
                     SMVNAGAEVIADGHIHVYAPLRGKAIAGARGNTEARIIALCLEPELISIAGVYRTSEV
                     PLPADVLSQPTQVRLVGGAEGKLVMEPLVPSPKGRGN"
     gene            2047..2517
                     /locus_tag="VGA59_03860"
     CDS             2047..2517
                     /locus_tag="VGA59_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718843.1"
                     /translation="MKMHHTRYYWIAVALAVAAFLLTLNEAHGQSAGAAAAFEGRPAM
                     AGAQAGQGAQAGPPQGGIGVQGTDVAERGLRLRKPSQLDDVRQARRDDSGIGADGSND
                     DVTARKEVAPPRDRSLAKDERSAVTKSKRAAKRTIKRARYGVSEIDSAAAATPR"
     gene            2599..2955
                     /locus_tag="VGA59_03865"
     CDS             2599..2955
                     /locus_tag="VGA59_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9718844.1"
                     /translation="MQDTKLTNVVRAYFGAYETENREIAETLLSDDFTFTSPNDDAID
                     KATYFERCWPNSDARHDQSIESIFVEGDKAFVTYHCATAGGKSFRNTEFLTFRGERIA
                     SVNVYFGAAYQDGVFV"
     gene            complement(3142..3843)
                     /locus_tag="VGA59_03870"
     CDS             complement(3142..3843)
                     /locus_tag="VGA59_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HEX9718845.1"
                     /translation="MEARLASETDVAGFREEARALLAHQVPPEAVHWHAEPAQNSDQF
                     ADPAAPGDSRPRGVPKAASAIVPASFLRLCEIVVLHSDPNRFALLYRLLWRLVHEPGL
                     RHNPVDPDMQHAHQMGQAVRRDMHKMKAFLRFRTMRDSQGNPMQLGWFDPAHHIVEAV
                     APWFARRSPQTRWAILTPQRSVEWDTHQLHFGPGLKRSDAPLPDADDAAWLACYQHVL
                     GEHRRAADAQGSVPT"
     gene            complement(3877..4899)
                     /locus_tag="VGA59_03875"
     CDS             complement(3877..4899)
                     /locus_tag="VGA59_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX9718846.1"
                     /translation="MTSRSLPRNALVKPRPRRAAPGHAGPHGDHPWWHWIKRALSLLF
                     FSAVAVLLVRYARTIDWGDVLDSVRNTPGPALLAAVVLAAASHLLYSCFDLFGRRYTG
                     HELPTGTTMAVTFVSYAFNLCIGAMVGGVGFRYRLYSRLGLGKGVITRVVTLSMLTNW
                     LGYMLLAGLLFLVWPLELPPSWKMGNHGLQWLGAALVLGAIGYVLACVRAGGRVWTVR
                     GHEIFLPPWRMAVQQLAISCVNWSLMGGVVYVLLQQKVAYTDVLTVLLIGAIAGVVAH
                     VPAGLGIFEFVFLALLSHQVSEGRLLAALLGYRAIYYIVPLMAASLLYLAMEAHARQR
                     RKRAWP"
     gene            complement(4903..6177)
                     /gene="clsB"
                     /locus_tag="VGA59_03880"
     CDS             complement(4903..6177)
                     /gene="clsB"
                     /locus_tag="VGA59_03880"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEX9718847.1"
                     /translation="MNDASRPLAAGAGSPVQSRWINGNAIQLLENGEEYFPRVFARIA
                     EAREEIVLETFILFEDKVGLQLHGALLAAARRGVQVDVTIDGFGSLDLSDDFIGSLVD
                     AGVRIHVFDPGKRLLGWRVNLLRRMHRKIVVIDRTIAFVGGINYSADHLADFGPAAKQ
                     DYAVEIRGPLVAEIHRFTHTALAQGQRYQRARQWWRWRRRLRTSPDNLPAVGSAAAMF
                     VVRDNGDHPSDIEHHYRIAIRDARERVIIANAYFFPGYRFIRELRHAARRGVDVRLIL
                     QGAPDMPIVRTAATMLYRHLLDAGVRIYEYCERPLHGKVALVDREWATVGSSNLDPLS
                     LALNLEANVIIRDRDFNEHLYERLDLMIQRSCRQIEVNLLEPLSGWHLVRSFLVYHLT
                     RRYPGWATWLPRHVPRLADRLRHAYSSRGGKE"
     gene            complement(6174..6941)
                     /locus_tag="VGA59_03885"
     CDS             complement(6174..6941)
                     /locus_tag="VGA59_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX9718848.1"
                     /translation="MTDTVAIAPPLTVMTVNTHKGFTTFNRKFILPELRDAVRKVGAD
                     IVFLQEVLGTHEAYGRKVNGWPDGPHYEFLADSIWPEFAYGRNMVYPKGHHGNAVMSK
                     FPIVHYQNHDVSIAGPEKRGLLHCVVTMPDRNLEVHAICVHLGLAEAHRQQQLELLCE
                     IVHTEVPPDAPLIVAGDFNDWRRRAHELLWREAGLKEVFVAAYGESAKTFPARFPMLS
                     LDRIYVRNASVHLPVVLPRRPWSHLSDHAPLAAEIHL"
     gene            7449..7991
                     /locus_tag="VGA59_03890"
     CDS             7449..7991
                     /locus_tag="VGA59_03890"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX9718849.1"
                     /translation="MNIKRLLIPAVAAALSLGACAADLRPAGAFVQGGLADHRTYSVT
                     AGLVWPWSWRRDFGTAQVSGITEAYVSHWSGRGATERQSFTQLGLVPLLRLRMDGGRS
                     PWFLEGGIGISVMDRKFRTQDKEFSTAFNFVDVIGVGRSIGADRRQELSLRISHISNA
                     GITKPNPGEEFLQLRYAMMF"
     gene            complement(8004..9074)
                     /locus_tag="VGA59_03895"
     CDS             complement(8004..9074)
                     /locus_tag="VGA59_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX9718850.1"
                     /translation="MTTVRKGQAPAPMARDAFHERFMQAFFDPAYQRESTALARLEEI
                     AWDAYRQGNKAPLTRKAGPGFADPEYELSVEWRETRDQLIAAQQEWSQPGTPSRVLLV
                     CGAARNDGTCPGEISKTFRLVQIAREVLQSEAMEVELLDLSRLTSDFDLHIHPCKGCV
                     STAMPLCHWPCSCYPNHALRQTGDWMNEIYRMWVRAHGVILMTPVYWYEAPSPLKLMI
                     DRLVCADGGNPDPTSTHGKKPDEAKALELKGWDYPKHLAGRTYGVVVHGDVAGAGGVR
                     RALCDWLDWMGLIAAGSRARLDRYIGYYQPYALSHDALDADAGLQEEVRNVARAVAAA
                     VGELRAGRLRAPERRLKDPRPK"
     gene            complement(9184..9672)
                     /locus_tag="VGA59_03900"
     CDS             complement(9184..9672)
                     /locus_tag="VGA59_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX9718851.1"
                     /translation="MKQPHKHLIVAGLLATLAFATSAQTPPAAPGAAPTERAYQHHGR
                     FDPARMQERVARRQAALKQKLQVTPAQEAAWSAYTAAMKPPADFRRPERGEFRQLTTP
                     ERIDRIRTMHAARAAEMDRRGEATKTFYAALSPEQKKVFDDATLRRGHGRHHGGRWHR
                     AA"
     gene            9917..10285
                     /locus_tag="VGA59_03905"
     CDS             9917..10285
                     /locus_tag="VGA59_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718852.1"
                     /translation="MKYPILMRGVALVAALGAGSVLAQARPSATDSGLTPPVVTDVGP
                     APAHERESSGAIILENSMVRAQRRHAFEHAASRTGVATVGRGVLRATMQAQREADLAQ
                     AREQEAVEMYRRGASGLTEK"
BASE COUNT         1708 a         3463 c         3632 g         1760 t
ORIGIN      
        1 aactcgatct tgacttccag cacctccagg ttgtcctggc gatcgacgtt gaccttgatg
       61 tcctgagggc cgatcttgat gtacttggag atcacggcga ccagttcgcg ctgcagcgcc
      121 ggcaggtagt cgggctggcc cgcgttgcgg ccgctgcgct cgtgcgccag gatgatctgc
      181 agccgctcct tggcgacgct ggcggtcttc ttcttttcgc ccagcaggaa ggaaaggaat
      241 gagcccatgc ttcacttgcc cccgaacagg cgcttgagga agccgccctt ttgcggatcg
      301 acgaaacgca tcggcgtgtc cttgccgaga aagcggtcga ccacgtcctt gtaggcttcc
      361 gagacctcgc tgcccttcat gtgcaccgcc ggcaggccct ggttggaggc ctgcagcacg
      421 gtttcgcttt ccgggatcac gccgatcagc ttgatgcgca ggatgtcctt gatgtcctcc
      481 agcgacagca tctggccctg gtccacgcgg ttcgggttgt agcgggtgat gagcaggtgt
      541 tccttgaccg gctccttgcc ctcgatggca cgccgcgtct tgctggcgag catgcccagg
      601 atgcggtcgg agtcgcgcac cgacgacacc tcggggttgg tgaccaccaa cgcctcgtcg
      661 gcgaaatgca tggccatcag cgcgccggtt tcgatgccgg cgggcgagtc gcagacgatg
      721 tagtcgaagc ccatgccgcc caggtcgttc aagacctttt ccacgccgtc cttggtcagc
      781 gcctccttgt cacgggtctg tgaggccgcc agcaccgaca ggttgtcgca ctgcttgtcc
      841 ttgatcaggg cctggtgcag gttggcctcg ccctggatca cgttgatcag gtcgtagacc
      901 acgcggcgct cgcagcccat gatcaggtcg aggttgcgca ggccgacgtc gaagtcgatc
      961 acggccgtct tgtggcccgc catggccatg cccgcggcaa agctggcgct ggtggtggtc
     1021 ttgccgaccc cgcccttgcc tgaagtcacg acgacgattc ttgccatagt gtgggttcct
     1081 ttatgaatta attccccctg cctttgggag agggaacaag aggctccatc accagcttgc
     1141 cttcggcgcc gcccaccagc cgcacctggg tcggttggga aaggacgtcg gcgggcaagg
     1201 gcacctcgct ggtgcggtac acgccggcga tggagatgag ttcgggttcg aggcacagcg
     1261 cgatgatgcg ggcctcggtg ttgccgcgcg cgccggcgat ggccttgccg cgcaacggcg
     1321 catagacatg gatgtggcca tcggcgatga cctcggcgcc ggcattcacc atgctgagca
     1381 tgaccaggtc gcggccgcgc gcatagatct gctggccgga gcgcagcggc ttgtcgacca
     1441 cgagcgccga caccggcacg agctcaggcg cgggagccgt cggggccttg cgggtacgct
     1501 ggggttccgg tgcggccgcc accgcttcgg gtgccgcggc caggcccgcc tgcagcgccg
     1561 ccgccatctg cgcggcgctg ccgccgcgca ccgccaccgg cagcatgcgg tagctgcgca
     1621 gcagcgccag cagcgcttcg aattcgagcg ccgtgtcgcc gcgctcctgc agcggcgaca
     1681 gatcgatcac cagcggatcg tcgtcgaaaa aatcggggat gtcgccgaag cgggccgcaa
     1741 gctccgcggt cagcgcacgc aggtcggacg acttgagcag cagcgccacc agcggcaggt
     1801 tggcgctctt gatttcgaaa gtggcaggtg cgcgtccggc gagggcgacg gtcattgaaa
     1861 cttattgtgg gtggcgaatg gctgctcgat attgcgagcc ctgtgcaaaa aaggccgcgc
     1921 tcaaaaagcg gcgaatctta acagccatat gccagagttg tcctacagat tctgttgcca
     1981 aatgcacctg cccgtggccc gcaatgccgt cacactggat atgcggcacc atttctagga
     2041 gttgccatga agatgcacca cacgcggtac tactggatcg cagtcgcctt ggctgtggcc
     2101 gccttcttgc tgacgttgaa cgaagcgcat ggtcagagcg ccggcgcggc ggcggcgttc
     2161 gagggcaggc ctgcgatggc cggtgcgcaa gccggccagg gcgcgcaggc cggcccgccg
     2221 caaggcggca tcggcgtgca gggcaccgac gtggccgagc gcggcctgag gctgcgcaaa
     2281 ccttcgcagc tcgacgacgt gcggcaggcc cggcgcgatg acagcgggat cggcgcggac
     2341 ggctcgaatg acgacgtgac cgcgcgcaag gaagtcgcac cccctcgcga ccgcagcctg
     2401 gccaaggacg aacgcagcgc cgtcacgaaa agcaaacgcg cggccaagcg caccatcaag
     2461 cgcgcgcgtt acggcgtgag cgaaatcgac agcgccgcag ccgcgacgcc ccgctaggcg
     2521 cttcggcttt cacgcgccgc cacaggggcg atagggctgg ctcgcaggcg ccgtaagcgc
     2581 aagcggttac catcgcccat gcaagacacc aaactgacga acgtcgtgcg cgcctatttc
     2641 ggcgcctacg agaccgagaa tcgcgaaatc gccgagaccc tgctctcgga cgacttcacc
     2701 ttcaccagcc ccaacgacga cgccatcgac aaggcgacat atttcgagcg ctgctggccc
     2761 aacagcgatg ccaggcatga ccagagcatc gagagcatct tcgtcgaggg cgacaaggcc
     2821 ttcgtcacct accattgcgc cacggccggc ggcaagtcgt tccgcaacac cgaattcctc
     2881 accttccgcg gcgagcgcat cgccagcgtt aacgtgtact tcggcgcggc ctaccaggac
     2941 ggcgtgttcg tctgagaccc ggttgcgggg ccgccagggg tttgtcatcc cgggcttgac
     3001 ccgggatcca gtgccctgcg gaatgaccgt cgccaaagtc atggatgccg gatcgggcct
     3061 gccccggact tgatccgggg tccggcatga caaccccttt cgacacgaac agcaggaaag
     3121 cttgttcccg cggctggtcc ttcatgtcgg cacgcttccc tgcgcatcgg cggccctgcg
     3181 gtgctcgccc agcacgtgct ggtagcatgc cagccaggcc gcgtcgtcgg catcgggcag
     3241 cggtgcgtcg gagcgcttga ggccgggccc gaagtgcagc tggtgggtgt cccattcgac
     3301 cgagcgctgg ggcgtgagaa tggcccagcg cgtctgcggc gagcgccggg cgaaccaggg
     3361 agccactgcc tcgacgatgt gatgggccgg atcgaaccag ccgagctgca tcggattgcc
     3421 ctggctgtcg cgcatggtgc gaaagcgcag gaaggctttc atcttgtgca tgtcgcggcg
     3481 caccgcctgg cccatctggt gcgcatgctg catgtcggga tcgaccgggt tgtggcgcag
     3541 cccgggctcg tgcaccagcc gccagagcag ccggtacagc agcgcgaagc ggttcgggtc
     3601 gctgtgcagc accacgatct cgcacagccg caggaacgag gccggcacga tggccgaggc
     3661 ggccttgggc acgccgcgcg ggcggctgtc gcccggcgcg gccgggtccg cgaactggtc
     3721 gctgttttgc gcgggctcgg catgccagtg cacggcttcg ggcggcacct gatgcgccag
     3781 cagcgcgcgt gcctcttcgc ggaagcccgc gacgtcggtt tcgctggcca accgcgcttc
     3841 cacttcagtt ccctttcgcc ggttcggcgg gcgctgtcat ggccaggccc ttttcctgcg
     3901 ctgcctggcg tgggcctcca tcgccaggta gagcagcgat gccgccatca gcggcacgat
     3961 gtagtagatc gcgcggtagc ctagcagcgc ggccagcagg cggccttccg aaacctggtg
     4021 cgacagcagc gccaggaaaa cgaattcgaa gatgcccagg ccggccggca cgtgggcgac
     4081 cacgcccgcg atcgctccga tcagcagcac cgtgagcaca tcggtgtaag ccaccttctg
     4141 ctgcagcagg acatagacta cgccgcccat caggctccag ttgacgcagg agatcgccag
     4201 ttgctgcacg gccatgcgcc aggggggcag gaagatctcg tggccgcgca cggtccagac
     4261 ccggccgccg gcacgcacgc aggccaggac atagccgatg gccccgagca cgagcgccgc
     4321 gcccagccac tgcaggccgt gattgcccat cttccagctc ggcggcaact ccagcggcca
     4381 gaccaggaag agcagtccgg caagcagcat gtagcccagc caattggtga gcatgctcaa
     4441 tgtcacgacg cgcgtgatca cgcccttgcc aagccccagg cgcgagtaca gccggtagcg
     4501 gaacccgacg ccaccgacca tggcgcctat gcacaggttg aacgcatagc tgacgaacgt
     4561 caccgccatg gtggtgccgg tgggcagctc gtgcccggta tagcgccgcc cgaacaggtc
     4621 gaagcagctg tacagcaaat ggctggccgc cgccaggacg acggctgcca gcaacgccgg
     4681 ccccggcgtg ttgcgcaccg agtccagcac gtctccccag tcgatggtgc gtgcgtagcg
     4741 caccagcagc acggcgacgg ccgagaagaa cagcagcgac agcgcgcgct tgatccagtg
     4801 ccaccaggga tggtcgccat gcggtccggc atggcccggc gcggcgcggc gcggccgtgg
     4861 cttgaccagc gcgttgcgag gcaaggaccg ggaggtcacg gttcactcct tgccgccgcg
     4921 gctggaatac gcgtggcgca gccggtcggc cagccgcggc acatgccgtg gcagccaggt
     4981 agcccagccg ggatagcggc gcgtcaggtg atagaccagg aagctgcgca ccaggtgcca
     5041 gccgctcagg ggctcgagca ggttgacctc gatttgtcgg cagctgcgct ggatcatgag
     5101 atccagccgc tcgtacaggt gctcgttgaa gtcgcggtcg cggatgatga cgttggcctc
     5161 caggttcagc gccaggctca acgggtcgag gttgctggag cccacggtcg cccattcgcg
     5221 gtcgaccagc gcgaccttgc cgtgcagggg ccgctcgcag tactcgtaga tgcgcacgcc
     5281 ggcgtccagc agatggcggt agagcatggt cgccgcggtc ctgacgatcg gcatgtccgg
     5341 cgcgccctgc aggatcaggc gcacgtcgac gccgcggcgg gccgcatggc gcagctccct
     5401 gatgaagcgg tagccgggga agaaataggc gttggcgatg atgacgcgct cgcgcgcgtc
     5461 gcggatggcg atgcggtagt ggtgctcgat gtcgctcggg tggtcgccgt tgtcgcgcac
     5521 cacgaacatg gcggcggcgc ttccgaccgc gggcaggttg tcggggctgg tgcgcaggcg
     5581 gcggcgccag cgccaccact ggcgggcgcg ctggtagcgc tggccctgcg ccagcgccgt
     5641 gtgggtgaag cggtggatct cggccaccag cgggccgcgg atctccaccg cgtagtcctg
     5701 cttggccgcg ggcccgaagt cggccaggtg gtcggccgaa tagttgatgc cgccgacgaa
     5761 ggcgatggtg cggtcgatca ccacgatctt gcggtgcatg cgccgcagca ggttgacgcg
     5821 ccatcccagc agccgcttgc cggggtcgaa cacatggatg cgcacgcccg catcgaccag
     5881 cgagccgatg aagtcgtccg acaggtccaa cgagccgaag ccgtcgatgg tgacgtccac
     5941 ctgcaccccg cgccgcgccg ccgccagcag cgccccgtgc aattgcagcc ccaccttgtc
     6001 ctcgaacagg atgaaggttt ccagcacgat ttcctcccgg gcctcggcga tgcgggcgaa
     6061 cacgcgcggg aaatactcct cgccgttttc gagcagctgg atggcgttgc cgtttatcca
     6121 gcgcgactgc acggggctcc cggcgccggc ggccaagggt cggctggcgt cgttcatagg
     6181 tggatctccg cggccagcgg ggcgtgatcc gacaggtgcg accagggccg gcgcggcagc
     6241 accaccggca gatggaccga ggcgttgcgc acatagatgc ggtccagcga cagcatcgga
     6301 aagcgggccg gaaaggtctt ggcggattcg ccgtaagccg cgacaaacac ttccttgagc
     6361 ccggcctcgc gccacaacag ctcgtgcgcg cggcggcgcc agtcgttgaa gtcgccggcc
     6421 acgatcagcg gcgcgtccgg cggcacttcg gtgtgcacga tctcgcacag cagctcgagc
     6481 tgctgctggc gatgcgcttc cgccaggccc aggtgcacgc agatcgcatg cacttccagg
     6541 tttcggtcgg gcatggtcac cacgcagtgc agcaggccgc gcttttcggg gccggcgatg
     6601 gagacgtcgt ggttctggta gtgcacgatc ggaaacttgg acatcacggc attgccatga
     6661 tgtcccttgg gatagaccat gttgcggccg tatgcgaact ccggccagat gctgtcggcc
     6721 aggaactcgt agtggggccc atcgggccaa ccgttcacct tcctgccgta ggcctcgtgc
     6781 gtgcccagca cttcctgcag gaacacgata tcggcgccca ccttgcgcac cgcgtcgcgc
     6841 agctcgggca ggatgaattt tctgttgaaa gtggtgaagc ccttgtgggt gttgaccgtc
     6901 atcacggtca acggcggggc gatggccact gtgtcggtca tgggtatccg gggaagggtt
     6961 gcgaaaaggg caatgtttct tgtactgctg cgttgaatac ccaggtgtag gacagcaggc
     7021 agacttgttg tcccggccgg cgcggatgcc gctgcgtcac gcggggcgcg tcgtgtcgat
     7081 gttgcgggtg gtgccgacca gcgtggcgca caggctttcc tcgccgctct cgctcaccgc
     7141 gaacacgttc cgcggcgcac acggtgagca gtttgccggc atcgatgacg cggccgcgtg
     7201 cacgcagggc aagcttcatc cggattcctt tgcgtggtgg cacaagctta ggaagcttcg
     7261 cgcggcgcga gggttgcgca ggtgactcgc gcgccggcgc ccaggcgact gctgtaggat
     7321 gacaacccgc cggcgggcag gagacggctg acggaacctg acagcgcatt tccatttttg
     7381 cgcctcaggc gttaccgggc gggcgcaagt ccacacggcg gcgccgctgt tggcgacact
     7441 gcaagcccat gaatatcaaa cggctcttga tcccggcagt tgcggccgcg ctgtcgctgg
     7501 gcgcctgcgc ggccgacctg cggccggccg gcgcgttcgt ccagggcggc ctggccgacc
     7561 accggaccta cagcgttact gccgggctgg tctggccctg gtcgtggcgg cgcgacttcg
     7621 gcaccgcgca ggtgagcggc atcaccgaag cctatgtgag ccattggagc ggacgcggcg
     7681 ccaccgagcg gcagtcgttc acccagcttg gtctggtgcc gctgctgcgg ttgcgcatgg
     7741 atggcggccg ctcgccctgg ttcctggagg gcgggatcgg gatctcggtg atggaccgga
     7801 aattccgcac ccaggacaag gaattcagca ccgcgttcaa cttcgtggat gtcatcggtg
     7861 tgggccgcag catcggcgcc gatcgccgcc aggaactgag ccttcgtatc agccacatct
     7921 ccaacgccgg catcacgaag cccaaccccg gcgaggaatt cctgcagctg cggtacgcga
     7981 tgatgttctg agcgccggtg ggcctacttg gggcgcgggt ccttgagccg gcgctccggg
     8041 gcgcgcaggc gtccggcgcg cagttcgccc accgccgcgg ccaccgcccg cgccacgttg
     8101 cgcacctctt cctgcagccc ggcgtcggca tcgagcgcgt cgtggctgag cgcgtaaggc
     8161 tggtaatagc cgatgtagcg atccagccgc gcgcggctgc cggcagcgat caatcccatc
     8221 cagtccagcc agtcgcacag cgcgcgccgc acgccgcccg cgcccgcgac gtcgccatgc
     8281 acgaccacgc cgtaggtgcg gcccgccagg tgcttgggat agtcccagcc cttgagttcc
     8341 agcgccttgg cctcatcggg tttcttgccg tgggtgctgg tgggatcggg attgccgccg
     8401 tcggcgcaga ccaggcggtc gatcatcagc ttgagcggac tgggggcttc ataccagtac
     8461 accggggtca tcaagatgac gccgtgcgcg cgcacccaca tccggtagat ctcgttcatc
     8521 cagtcgccgg tctggcgcag cgcgtggttg ggatagcagc tgcagggcca gtggcacagc
     8581 ggcatggccg tcgagacaca tcccttgcac ggatggatgt gaaggtcgaa gtcggacgtg
     8641 agccggctca agtccagcag ttcgacttcc atcgcttcgc tctgcagcac ctcgcgcgcg
     8701 atctgcacca gccggaaggt cttggatatt tcgccggggc aggtgccgtc gttgcgcgcc
     8761 gcgccgcata ccagcagcac gcgtgagggc gtgcccggct gcgaccactc ctgctgcgcg
     8821 gcgatgagct ggtcgcgcgt ctcgcgccac tcgaccgaga gctcgtactc ggggtcggca
     8881 aaaccggggc cggccttgcg cgtgagcggt gccttgttgc cctgccggta agcgtcccag
     8941 gcgatctctt ccaggcgcgc cagagctgtg ctttcgcgct ggtaggccgg gtcgaaaaag
     9001 gcttgcatga agcgctcgtg aaacgcgtcg cgcgccatgg gggccggcgc ctggcctttg
     9061 cgaacggtgg tcatcaaacc agtgtaggct gctgtcgcgg cacttggggc tcagccactg
     9121 agcgctggcg tcgatgtcct acagatggcg tcacgggcgt ccgagcttcg agctgcccga
     9181 agctcaggcc gcgcgatgcc agcgtccgcc gtggtgccgg ccgtggccgc ggcgcagggt
     9241 tgcgtcgtcg aagaccttct tctgctcggg cgaaagcgcc gcatagaagg tcttggtggc
     9301 ctcgccgcgc ctgtccattt cggcggcgcg cgcggcatgc atcgtccgga tacgatcgat
     9361 ccgctcggga gtggtgagtt ggcggaattc gccgcgctcg ggccgcctga aatcagccgg
     9421 cggtttcatc gccgccgtgt aggcgctcca tgcagcttcc tgcgccggag tgacttgcag
     9481 tttctgcttc aacgcggcct gccgcctggc cacgcgctcc tgcatccggg ccggatcgaa
     9541 gcggccatgg tgttgatacg ccctctccgt gggcgcggcg cccggcgctg ccggcggcgt
     9601 ttgcgcgctg gtggcgaagg ccagggtggc gagcaggccg gcgacgatca ggtgcttgtg
     9661 cggttgtttc atagcccttg cttcctttcc ttgcttgaag ggcctgacgc ccacgacggg
     9721 ctacaggttg ggctcgagtg tgaaacgctc atgtatagcc gccgtcgccg cgaagtagct
     9781 gatgtgtaaa gcctggatcg ggctgaacgc ggactgtggg agtgctccta ccgcaaaaac
     9841 gcaagaccgg ccacatccgg cggccgagca ggcgcgcacc atcacggtga cgaacgcttg
     9901 gatcaaggag catctcatga agtaccccat cttgatgcgc ggcgttgccc tggtggccgc
     9961 gctgggggcg ggcagcgtgc tcgcccaggc gaggccgtcg gccaccgaca gcgggctcac
    10021 gccgccggtc gttaccgatg tcggcccggc accggcgcat gagcgcgaga gttcaggcgc
    10081 gatcatcctc gagaactcga tggtgcgggc gcagcgccgg cacgcattcg aacacgccgc
    10141 gtcgcgcacg ggcgtcgcaa cggtcggccg cggcgtgctg cgcgccacga tgcaggcgca
    10201 gcgggaggcc gatctggcgc aggcgcggga acaagaggcg gtggagatgt accgccgcgg
    10261 cgccagcggc ttgacggaga agtagagatt gtcattgcgg gctgggctcg cgtaacgccc
    10321 tgggcgcgca gctgtccccg caatccatga attcagaaga catggatccc ggatcgagtc
    10381 cgggatgacc ccctttcttg gcgaggaccc ggggacgacc accatctcag ttgctccacc
    10441 gcgcgccgcg cgacccgcga cgcatgaaac ggatgtgtaa ttgggctgcg gcgtccggca
    10501 cagaggtcgg tttgtacttg gtgcctcgaa cccgtatctc agttggaccc gcggcctcac
    10561 gag
//
LOCUS       DASWIY010000101         5851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_426370, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000101 DASWIY010000000
VERSION     DASWIY010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426370"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VGA59_03910"
     CDS             complement(<1..928)
                     /locus_tag="VGA59_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX9718853.1"
                     /translation="MSCHWMRRALLALASASALLVVACGSGTIESQLQPSRIVAFGDG
                     FSDVGQGGSRYTVNDGSVNIWAHQVAASFGLPLTPVSAGGTSYATGNVRISSKPDAAG
                     SSATPTVTEQIDQFLAGEPIGANDLLIIGGGISDIVAEAANVNSAVQSGDQMVANVKQ
                     AGRDLGAQVRRLVQAGAAHVVVVGTYDLGRSPWATSTSQASLLSEASGRFNDQLLLSI
                     VDLGANVLYVDAALLFNLMISSPPSYDLTNVADPVCASVDPGPGIGIGAGQVNSALCT
                     TGTLVAGADYTRYLFADRVYPTPQGHRKFGEYA"
     gene            complement(975..1970)
                     /locus_tag="VGA59_03915"
     CDS             complement(975..1970)
                     /locus_tag="VGA59_03915"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX9718854.1"
                     /translation="MILAGASPAAIEQAAQALADGELVAFPTETVYGLGADAGSDRAV
                     AKIFEAKGRPSDHPLIVHVADESAVAMFASDVPDFAHRLMQAFWPGPLTLILPRRSGV
                     ASAAAGGQDSIGLRCPAHPVAHALLRACGRANPPVSGLAAPSANKFGRVSPTTAEHVR
                     DEFGEGLLVLDGGPCRVGIESTIVDCTRGSPVLLRPGVLTQGEVEAGCGQPLRAPGEL
                     EDLEPRASGTLQSHYAPNAAVRLMDAQALQTALDLLGSDAAGIAIYSRAIMRTPSARV
                     LRRRMPDDAAETARQLFAVLREFDAQGVRLIWVETPPEGPQWEGVRDRLQRASAA"
     gene            complement(1985..3142)
                     /locus_tag="VGA59_03920"
     CDS             complement(1985..3142)
                     /locus_tag="VGA59_03920"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX9718855.1"
                     /translation="MTAILPGATLGVMGGGQLGRMFVHAAQRMGYFTAVLDPDPASPA
                     GLVSHYHIQTNYLDQQGLAQLMQRSAAITTEFENVPAPALVTLGAHRPVAPGAEAVAI
                     AQDRAQEKAHFARCGVPCAPYTPIETPEHLAAAKAELLPGVLKTSRLGYDGKGQVQVR
                     KRDELQGAWDDLKRVPCVLEKLLPLAAEFSVIVARGHDGQMVHLPPQLNRHRGGILAV
                     TEVYPQSVPAPQARHAVTAAKAVAEELGYVGVLCIEFFVLADGSLVVNEMAPRPHNSG
                     HYSMDACDVSQFELQVRALAGLPLPQPRQHSPAIMLNLMGELWLAGGESERTPPWDQL
                     LALAGTHLHLYGKLSPRKGRKMGHVTITHPTVEAVRATAMKAADLLGIEPF"
     gene            complement(3139..3666)
                     /gene="purE"
                     /locus_tag="VGA59_03925"
     CDS             complement(3139..3666)
                     /gene="purE"
                     /locus_tag="VGA59_03925"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX9718856.1"
                     /translation="MSAIQVGVVMGSASDWETLQHAVAILQEFGIAHEARVLSAHRMP
                     DDMFAYAQSAGARGLKAIIAGAGGAAHLPGMLAAKTTVPVLGVPVAGKHLQGVDSLHS
                     IVQMPKGIPVATFAIGNAGAANAALFAVAMLANEDATLRARLEAFRARQTEEARGMSL
                     PVGLPAPPPGMGKGQ"
     gene            3793..4692
                     /gene="trxA"
                     /locus_tag="VGA59_03930"
     CDS             3793..4692
                     /gene="trxA"
                     /locus_tag="VGA59_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9718857.1"
                     /translation="MIDVTLENFEAEVIAASMSQPVLVDFWAPWCAPCKVIGPVLEKL
                     EADYAGRFKLVKIDSDQEQQLAGAFGIRSIPTCVLLVNGQPVDGFMGALPEGKVREFL
                     EKHVPPAAPQTEEEPVEEEAAGQADDAAPLEKLQHAVATDPANDEARSDYVKALLLAG
                     RTDDAKVAFAPVIGKTSTVRRFDSLQRFMDAIDCAAQPDAPARAGELDARIAANKRDF
                     EARFGKARLLMARQRWTEAMDELLEILMRDKAWNEDLARKTYIAILDIIEPPRPKVAE
                     GQIPPDDPTVATYRRRLSSVVLS"
     gene            complement(4744..5649)
                     /locus_tag="VGA59_03935"
     CDS             complement(4744..5649)
                     /locus_tag="VGA59_03935"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9718858.1"
                     /translation="MTALHTSALTSLPLLARGKVRDNYAVGNDRILMVASDRLSAFDV
                     IMGEPIPGKGEILTQMALFWFGKLGHLCPNHLTGEAPESVVTPAEVPQVAGRSMLVKR
                     LKPIPVEAVVRGYLAGSGWKEYQDSQAICGVRLPPGLKNAGKLPQPIFTPAAKAAAGQ
                     HDENISYERVVALIGPELAARIRDISIAVYKEAAAMALAKGMIIADTKFEFGLDDAGT
                     LVLMDEVLTPDSSRYWPLEGYDEAFRTGVNPPSFDKQFVRDWLEQVRINGKPWDKTPP
                     APRLPKDVIDRTAAKYREALQRLAA"
BASE COUNT          934 a         2003 c         1990 g          924 t
ORIGIN      
        1 aggcgtattc gccgaacttg cgatgtccct gcggggtcgg gtagacgcgg tccgcgaaca
       61 ggtagcgggt gtagtcggcg cctgccacca gggtgccggt ggtgcacaaa gccgagttca
      121 cctggccggc gccgatgccg atgccggggc cggggtccac cgaagcgcag accgggtcgg
      181 ccacgttcgt caggtcgtag gacggggggc ttgaaatcat caggttgaat agcagcgcgg
      241 catccacgta cagcacgttg gcgcccaggt ccacgatcga caacagcagc tgatcgttga
      301 acctgccgct ggcctccgac aacaggctgg cctggctggt cgacgtagcc cagggcgacc
      361 ggcccaagtc ataggtgccg accaccacca cgtgcgcggc gcccgcctgg accagccggc
      421 gcacctgagc gcccagatcg cgcccggcct gtttcacgtt cgccaccatc tggtcgccgg
      481 actggactgc cgagttcacg ttggcggctt cagcgacgat gtcgctgatg ccgccaccga
      541 tgatcagcaa gtcgttggcg ccgatgggtt cgccggccag gaactggtcg atctgctcgg
      601 tgaccgtcgg ggtggcgctg ctgccggccg catcgggctt gctgctgatg cggacattac
      661 cggttgcgta cgaggtgccg cccgcggaaa ccggcgtcag gggcaggccg aagctggcgg
      721 ccacctggtg tgcccagata ttgacgctgc cgtcgttgac cgtgtagcgc gagccgcctt
      781 gccccacatc gctgaagccg tcgccgaatg cgacgatgcg cgagggctgc agctgcgact
      841 cgatggtgcc cgaaccgcag gcgacgacca acagcgccga ggcggacgcc agcgccagca
      901 gggcgcggcg catccagtga catgacatga aattctccga taagactggg gacgtagttt
      961 aacgaggccg cctgtcacgc cgcggacgcc ctttgcaggc gatcgcgcac gccctcccac
     1021 tgggggccct cgggcggcgt ctccacccag atcagccgga ctccctgggc atcgaactcg
     1081 cgcagcacgg caaacagctg gcgcgcggtc tccgccgcgt cgtcgggcat tcgccggcgc
     1141 agcacgcgag ccgaaggcgt gcgcatgatg gcgcgcgagt agatggcgat gccggcggcg
     1201 tcggatccga gcaggtccag cgcggtctgc agcgcctgcg cgtccatcag cctgaccgcg
     1261 gcgttgggcg cgtaatgcga ttgaagcgtg ccggaagcac gcggctccag gtcctcgagc
     1321 tccccggggg cgcgcagcgg ctgaccgcag cccgcctcga cctcgccctg tgtcagcacg
     1381 ccggggcgca gcagcacggg gctgccccgg gtgcagtcca cgatggtcga ttcgatgccg
     1441 acccggcagg ggccgccatc gagcaccagc agcccctcgc cgaattcgtc ccgcacatgt
     1501 tccgcggtgg taggactgac gcggccgaac ttgttggcgc tgggtgccgc caaccccgac
     1561 accggcggat tcgcccggcc gcaggcccgc agcagcgcat gcgccaccgg atgggccgga
     1621 cagcgcaagc cgatcgagtc ctgtccgccg gcggcggccg acgccacgcc gctgcgccgc
     1681 ggcaggatca gcgtcagcgg tcccggccag aatgcctgca tcagccggtg cgcgaagtcg
     1741 ggcacatcgg acgcgaacat cgccacggcc gattcatcgg ccacgtgcac gatcagcggg
     1801 tgatcgctgg ggcggccctt ggcctcgaag attttcgcca cggcccggtc gctgccggca
     1861 tcggcgccca gcccgtaaac ggtttcggtc gggaaggcca ccagctcgcc gtccgccagc
     1921 gcctgggcgg cctgctcgat cgccgcgggg gaagcgccgg ccaggatcat gggtcgggcc
     1981 ggcgtcagaa cggctcgatg cccaacaggt cggccgcctt catggccgtg gcgcgcaccg
     2041 cctccaccgt ggggtgggtg atcgtgacgt ggcccatctt gcggcccttg cgcgggctga
     2101 gcttgccgta caggtgcagg tgtgtaccgg caagcgccag caattgatcc cacgggggtg
     2161 tgcgctcgct ctcgccgccg gccaaccaca gctcgcccat caggttgagc atgatggccg
     2221 ggctgtgctg acgcggctgc ggcaacggca agcccgcgag cgcgcggacc tgcagctcga
     2281 actgcgaaac gtcgcaggcg tccatgctgt agtggccgct gttgtgcggc cggggcgcca
     2341 tctcgttgac caccagcgag ccatcggcca gcacgaagaa ctcgatgcac aaaacgccca
     2401 cgtagcccag ttcctccgcg acggccttgg ccgcggtcac cgcgtggcgg gcctggggcg
     2461 ccggaacgct ctgcggatag acctcggtca ccgcgaggat gccgccgcgg tgccggttca
     2521 actgcggcgg caggtgcacc atctgtccgt cgtggcccct ggccacgatc accgaaaatt
     2581 ccgctgccag cggcagcaat ttttcgagca cgcaaggcac tcgcttgaga tcgtcccatg
     2641 cgccttgcag ctcatcgcgc tttctcacct gcacctggcc tttgccgtca tagcccaggc
     2701 gggaggtctt gagcacgccc ggcagcagct cggccttcgc cgccgccaga tgctcgggcg
     2761 tttcgatcgg cgtgtacggc gcgcaaggga cgccgcagcg cgcgaaatgg gctttttcct
     2821 gggcccggtc ctgcgcgatg gccaccgcct cggcgccggg cgccacaggc cggtgcgcgc
     2881 ccagcgtcac cagcgccggc gccggcacgt tctcgaactc ggtcgtgatc gccgcgctgc
     2941 gctgcatcag ttgcgcaagt ccctgctggt cgaggtagtt ggtctggatg tggtagtggc
     3001 tcaccagccc ggccgggctg gccggatcgg gatccagcac cgcggtgaaa tagcccatgc
     3061 gttgggctgc atggacgaac atgcgtccca gctggccacc gcccatcacg ccgagcgtgg
     3121 cgcccggcag gatggccgtc attgcccttt ccccatgccg gggggcgggg ctggaaggcc
     3181 caccggcaga ctcatgccgc gtgcctcctc ggtctggcgg gcccggaacg cttccagcct
     3241 ggcccgcaac gtggcgtctt cgttggccag catggccacc gcgaacagcg ccgcgttggc
     3301 ggcgccggcg ttgccgatag cgaaggtggc aaccggaatg cccttgggca tctggacgat
     3361 gctgtgcagc gaatccacgc cctgcaggtg cttgccggcc accggtacgc ccagcacggg
     3421 caccgtcgtc ttggccgcca gcatgcccgg caggtgggcg gcgccacccg cgccggcaat
     3481 gatggccttc aggccgcgcg cgccggcgga ctgggcgtag gcgaacatat cgtcgggcat
     3541 ccggtgggcc gatagcacgc gggcctcatg ggcgatgccg aattcctgga gaatcgcaac
     3601 tgcgtgctgc aaagtctccc agtcgctggc cgaacccatc accacgccaa cctgtatagc
     3661 gctcatgccg cctcctgccg agccgcctca aaggcggtta gccccatcgg ggggcaggga
     3721 acgtcatggg catggaggtt catagaattc ctaaaggttg aattttaagg ttccaaccaa
     3781 agctgcctgc tcatgatcga cgttaccctg gaaaactttg aagccgaggt catcgccgcc
     3841 tcgatgtcgc aaccggtgct ggtggacttc tgggcgccct ggtgcgcgcc ctgcaaggtc
     3901 atcggacccg tgctggaaaa gctggaggcc gattacgccg gccgcttcaa gctggtcaag
     3961 atcgactccg accaggaaca gcagctggcg ggcgccttcg gcatccgcag catcccgacc
     4021 tgcgtcctgc tggtgaacgg ccagcccgtg gacggcttca tgggcgcgct gcctgaaggc
     4081 aaggtgaggg aattcctcga gaagcacgtg ccgccggccg cgccgcagac cgaagaggaa
     4141 ccggtcgagg aagaggcagc gggccaagcg gacgacgcgg ccccgctgga aaagctccag
     4201 catgcggtgg ccaccgaccc ggcgaacgac gaagcccgct ccgactacgt caaggccttg
     4261 ctgctggcgg gccgcaccga tgatgccaag gtggcgttcg cccccgtgat cggcaagaca
     4321 tccaccgtgc ggcgcttcga ttccctgcag agattcatgg atgccatcga ttgcgcggcg
     4381 cagccggacg cgccggcccg ggcgggcgaa ctcgatgccc gcatcgccgc caacaaacgc
     4441 gacttcgagg cacggttcgg caaggcccgg ctgctcatgg cgcggcagcg ctggaccgaa
     4501 gcgatggacg agctgctgga aatcctgatg cgcgacaagg cctggaacga ggatctggcg
     4561 cgcaagacct acatcgccat cctcgacatc atcgagccgc cccgccccaa ggtggcggag
     4621 ggccagatcc cgccggacga ccccacggtg gcgacctatc gccgccgcct gtccagtgtg
     4681 gtgctgagct gacgcatcaa agcgcaacgg gtgtggccgt cgcttgcggc aaggtctctt
     4741 ttctcaggcc gcgagccgct gcagcgcctc gcggtatttc gccgccgtcc tgtcgatcac
     4801 atccttcggc aggcgcggcg ccggcggcgt cttgtcccag ggcttgccgt tgatgcgcac
     4861 ctgctccagc cagtcgcgca cgaactgctt gtcgaagctc ggcggattga cgccggttcg
     4921 aaaggcctcg tcgtagcctt ccagcggcca gtagcgcgaa gagtccgggg tgagcacctc
     4981 gtccatcagg accagggtgc cggcatcgtc cagtccgaac tcgaacttgg tgtcggcaat
     5041 gatcatcccc ttggccaggg ccatcgcggc agcctccttg tagaccgcga tgctgatgtc
     5101 tcggatccgg gccgccagct cgggcccgat cagtgccacc acccgctcgt agctgatgtt
     5161 ctcgtcgtgt tgaccggccg cggccttggc tgcgggcgtg aagatcggct gcggcagctt
     5221 gccggcgttc ttcaatcccg gcggcagcct cacgccgcag atggcctgcg agtcctggta
     5281 ctccttccag ccgctgcccg cgagatagcc gcgcacgacg gcctccaccg gaatcggctt
     5341 gagccgcttg accagcatcg agcggcccgc gacctgcggg acctcggccg gcgtcacgac
     5401 gctctcgggt gcctcgccgg tcaggtggtt ggggcacagg tggcccagct tgccgaacca
     5461 gaacagcgcc atctgcgtga ggatttcgcc cttgcccggg atcggttcgc ccatgatcac
     5521 gtcgaaggcc gacagccggt cgctggcgac catcaggatg cggtcgttgc cgacggcata
     5581 gttgtcgcgc accttgccgc gcgcgagcag cggcagggat gtgagagcgg aggtgtgcag
     5641 ggcggtcatg ggcgaggcct ggaaagcaaa agggcccgct gaagatcaac gggccccttt
     5701 attatcccgt catctcagtt gctccaccgc gcgccgcgcg acccgcgacg catgaaacgg
     5761 atgtgtaatt gggctgcggc gtccggcaca gaggtcggtt tgtacttggt gcctcgaacc
     5821 cgtatctcag ttggacccgc ggcctcacga g
//
LOCUS       DASWIY010000102         3731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1541378, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000102 DASWIY010000000
VERSION     DASWIY010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541378"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VGA59_03940"
     CDS             <1..419
                     /locus_tag="VGA59_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX9718859.1"
                     /translation="CPWLIARFIDPEAEFLYVPPKAVLDTATSKGAIPYDVPDVQFAH
                     DGALCSFDAFLKTYRLTDDPALARLAVIVRGADTARLELAPQAAGLLAVSLGLSRTFK
                     DDHEMLKHGMVVYDALYAWCKEGQDETHTWKPDAYR"
     gene            456..1667
                     /locus_tag="VGA59_03945"
     CDS             456..1667
                     /locus_tag="VGA59_03945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9718860.1"
                     /translation="MGTLTSLASLCAVGFLARASYALARTPVLALFAVALGAGPEAAG
                     LAVGISTVTGILFKMPAGVISDVVGRSRMLLLGLCVFAFVPFAYLWIESYPALVAVRF
                     LHGFATAIYGPVSMAVVVAAAGAKRAEGLAWFSSITIIGNLIGAPLGGWLLTAQAGGG
                     SATLAHFHVVYAVVAALGSGALVLALWALRGRLSDVPANGQGTVVAAWKRFQTGVREI
                     LLDRRVLLTSNMEGVQNLSVGALEAFLPVYAVVVCGFSPLLAGLLWAVQAVTTLAAKP
                     LMGRWSDQRGRHGLLFWGMFLCAVPFALIPWTREFNLLLVLSAVFGLGEAVVTSSAAA
                     LVADLCGESELGAAMGAFGTLFDVGHAAGPILAGMLLVLSGDQDFRPAFAIIAALLVL
                     AALAFRWGVPK"
     gene            1748..2341
                     /locus_tag="VGA59_03950"
     CDS             1748..2341
                     /locus_tag="VGA59_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX9718861.1"
                     /translation="MSYVMKPLPCDPSKLTGLSEKLIVSHYENNYGGAVKRLNTICDQ
                     LASLDPTSAPVFVINGLKREQLIAMNSMILHELYFDGLGARGDPGGALSDAIKRDFGG
                     FERWRSEFVAMGKALGGGSGWVLLTYAHRDGQLINQWAADHTHVVAGATPILALDMYE
                     HSYHMDYGAKAAAYVDAYMENINWTNVAKLHGRCVQR"
     gene            complement(2380..3474)
                     /locus_tag="VGA59_03955"
     CDS             complement(2380..3474)
                     /locus_tag="VGA59_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX9718862.1"
                     /translation="MRRFRTIGDGSGFGRDDTLYPEELQLALRNRGMPLEGLRYPITP
                     TGIHYLLVHYDIPEIDASAWRLTVSGLVTTPLSLTLDDIRKRPRRTLAVTMECAGNGR
                     ALSSPRRISQPWLVEAIGTAEWTGMPLKGVLEEAGLARAAIEVIFTGRDEGVEGNVVQ
                     RYQRSLAVSEALRDEVLLAYEMNGQPIEPQHGFPLRLVVPGWYGMTSVKWLDRIDATA
                     EPFQGYQMTRVYRYARSEEESGEPVTLIRVRALMIPPGIPDFMTRTRLVRAGRVILSG
                     RAWAGRLAVARVEVSADGGATWSDARFGDPVSPHAWNEWIFEWNARPGSYTLCVRATD
                     SSGNVQPAEQEWNFGGYGNNSVQRVDAIVE"
     gene            3528..>3731
                     /locus_tag="VGA59_03960"
     CDS             3528..>3731
                     /locus_tag="VGA59_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX9718863.1"
                     /translation="MDPGSAVSVPSAKSRTDPFPSPQAAGLLAVSLGLSRNFRDDHEM
                     LKHGMVMYDALYAWCKEGQDETHT"
BASE COUNT          588 a         1209 c         1197 g          737 t
ORIGIN      
        1 cctgcccgtg gctcatcgcg cgcttcatag accccgaagc cgagtttctt tacgttccgc
       61 cgaaagcagt cctcgatacg gcgacgtcga agggggcgat tccttatgac gtccccgacg
      121 tgcagttcgc gcacgatggc gcgctgtgca gcttcgatgc tttcctcaag acctaccggc
      181 tcacggatga ccccgcactg gcccggctcg cggtgattgt gcgcggcgca gacacggcgc
      241 gacttgaact ggcgccgcag gcagcgggat tgctcgccgt ctcactgggc ctctcacgga
      301 ctttcaagga cgaccacgaa atgctcaagc acgggatggt ggtgtacgac gcgctctacg
      361 cctggtgcaa ggagggacag gatgaaacgc acacctggaa gcccgacgcc tatcgttgat
      421 gcccgcaggt attcctaccc gttacgtccc ccgtcatggg tacgctgacc tcactggcca
      481 gtctgtgcgc cgtgggcttc ctggcacggg cctcctatgc gctggcgcgc acgccggtgc
      541 tggcgctgtt cgccgtggcc ttgggagcgg ggccagaggc ggcgggactg gccgttggta
      601 tttccaccgt taccggcatc ttgttcaaga tgcccgcggg cgtgatctcg gacgtggtgg
      661 gccgctcgcg catgctgctg ctgggcctgt gcgtgttcgc cttcgtgccc tttgcctacc
      721 tgtggatcga gagctatccg gccctggtcg ccgtacggtt tctgcacggt tttgccaccg
      781 ccatctatgg tcccgtttcc atggccgtgg ttgttgccgc cgccggtgcc aagcgcgcag
      841 agggtctggc ctggttctcg tccatcacca tcatcggcaa cctgatcggc gcccctcttg
      901 ggggctggct gctcaccgcg caggcgggcg gtggcagtgc cacgctcgcc cactttcacg
      961 tcgtctacgc ggtggtggcc gcactgggca gcggcgcgct ggtcctggcg ctatgggcgc
     1021 tgcggggccg gctgagcgac gtgcctgcca atgggcaagg caccgtggtt gcggcctgga
     1081 agcgttttca gaccggcgtg cgggaaatcc tgctggaccg gcgcgtgctg ctcaccagca
     1141 acatggaggg cgtgcagaat ctttcggtcg gcgcgctgga ggcttttctc cccgtctacg
     1201 cggtggtggt gtgcggcttt tcgccgctcc tggcgggcct gctctgggcc gtgcaggccg
     1261 tgacgacgct ggccgccaag cccctgatgg gccgctggtc cgaccagcgc gggcggcacg
     1321 ggctgctgtt ctggggcatg ttcctgtgcg cggttccttt cgccctcatc ccctggacgc
     1381 gggagttcaa cctgctgctc gtgctcagcg cggtgttcgg cctgggcgaa gcggtcgtca
     1441 cgtcgtccgc ggcggcgctg gtggccgacc tgtgcggaga gagcgagctg ggcgcggcga
     1501 tgggcgcctt cggcacgctg ttcgatgtcg gacatgccgc aggaccgatc ctggccggca
     1561 tgctgctggt gttgtcgggt gatcaggact ttcgccccgc tttcgccatc atcgccgcgc
     1621 tgctggtgct ggcggcgctt gcattccggt ggggcgtgcc gaaatagccg gcgcgattct
     1681 cgacctggca cgatcgtgac gtattatttc ggcgttcgtt gaatatacag cgtctgcggg
     1741 aggtgatatg agctacgtca tgaaaccgtt gccctgcgat ccctcgaaac tgaccgggct
     1801 gtccgaaaag ctgatcgtga gccactacga gaacaactac ggcggtgcgg tcaagcggct
     1861 caacacgatt tgcgatcagc tcgccagcct cgatccgacg agcgcgccgg tgttcgtgat
     1921 caacggcctc aagcgcgagc agctgatcgc gatgaactcc atgatcctcc acgagcttta
     1981 cttcgatggt ctgggcgccc gcggtgatcc gggaggcgcc ttgtcggatg cgatcaagcg
     2041 ggatttcggc ggtttcgagc gctggcgcag cgaattcgtg gccatgggca aagcgctcgg
     2101 aggtggatcg ggctgggtgc tgctcactta cgcccaccgc gacggacaac taatcaatca
     2161 gtgggcggcc gatcacacgc atgtcgtggc gggcgcaaca ccgatcctcg cgctcgacat
     2221 gtatgagcat tcgtaccaca tggactacgg cgccaaggct gccgcttacg ttgacgccta
     2281 catggaaaac atcaactgga cgaacgtggc gaagctccac gggcgatgcg tccaaagata
     2341 atcttctgac actaaagaaa tggggtcgac cccatgtctt cactcgacga tcgcatccac
     2401 ccgctgtacg ctgttgttgc cgtagccacc gaagttccat tcctgctccg ccggctgcac
     2461 gtttccggac gaatcggtcg cccgcacgca cagcgtgtag ctgccaggcc gcgcgttcca
     2521 ttcaaaaatc cattcgttcc aggcgtgagg cgacaccggg tcgccaaagc gggcgtctga
     2581 ccaggtcgcc ccgccgtccg cgctgacctc gacacgtgcc acggcgaggc ggccggccca
     2641 cgcccggccg gagaggatca ctcggccggc gcgaaccagc cgcgtccgcg tcatgaagtc
     2701 cgggatcccc gggggaatca tgagcgcgcg taccctgatg agcgttaccg gctctccgga
     2761 ctcttcctcc gagcgggcgt accggtagac acgcgtcatc tggtatccct ggaaaggctc
     2821 ggccgtcgcg tcgatccggt ccaaccattt cacgctcgtc attccgtacc agcctggcac
     2881 gaccaggcgc agcggaaatc cgtgctgcgg ctcaatcggc tgtccgttca tttcataggc
     2941 cagcaggact tcatcgcgga gcgcctcgct caccgcgagg ctgcgctggt agcgttgcac
     3001 cacgttgccc tccacgccct catcccgccc ggtgaaaatc acttcaatcg ccgcacgggc
     3061 gagtcccgct tcctccaaaa cacccttgag cggcataccg gtccattcgg ccgtcccgat
     3121 ggcctccacc agccacggct gactgatccg gcgcggcgac gacagcgcgc ggccgttgcc
     3181 tgcgcattcc atcgtgaccg ccagcgtgcg cctcggccgc ttgcggatat catcgagcgt
     3241 cagactgagt ggcgtggtga ccaggccgct gaccgtgagt cgccatgcgc tcgcatcgat
     3301 ttccgggatg tcgtagtgga cgagtaggta atggatgccg gtcggcgtga tcgggtagcg
     3361 caacccttcg agcggcattc cccggttgcg cagcgcaagc tgcagctcct cagggtagag
     3421 agtatcgtcc cgtccaaacc cgctgccgtc gccgatggtg cggaagcgcc tcactgtgat
     3481 tttgcgtcct cagtggttca ctgcacgggg tcagttcttg actttgcatc gatccaggat
     3541 cagctgtctc ggtcccaagt gcaaagtcaa gaactgaccc ctttccttca ccgcaggccg
     3601 cggggctgct cgcggtgtca ctgggactgt cccggaattt cagggacgat cacgagatgc
     3661 tcaagcacgg catggtgatg tacgacgccc tctacgcctg gtgcaaggaa ggtcaggatg
     3721 aaacgcacac g
//
LOCUS       DASWIY010000103        19567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1920422, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000103 DASWIY010000000
VERSION     DASWIY010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 19567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19567
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920422"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VGA59_03965"
     CDS             <1..468
                     /locus_tag="VGA59_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HEX9718864.1"
                     /translation="LFQALEKGVLDGTEFPLPTVDEQLGFSKVAKNYYMPGWHQPSTN
                     QFLYVNMAAWTKLKPQTKAQIETTCTAGVTMAIAKAEALQGQTLTKFEKDGVKARQFN
                     KVMLDAFAKATKEVMAEESAKDPLFKKVYDSMVAFQQKNQAWHHLGYLPRDHK"
     gene            563..1138
                     /locus_tag="VGA59_03970"
     CDS             563..1138
                     /locus_tag="VGA59_03970"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9718865.1"
                     /translation="MESHLHEVEELIAGHSGIGLPVTGFSHAVDRVIEWVGEGASILW
                     TILVLVIVFQVFLRYALGLGSIMLEETQWHLYAVGFLLGLSFTEIRERNVRIDGLAEG
                     LQRRTRLWIELIGIAFFLLSFSLLVIWFAVPYVITSWELSEVSAAPGGLPYRWALKSF
                     LITAFALLALAGASRLTRVWAALFGQRAIPS"
     gene            1163..2533
                     /locus_tag="VGA59_03975"
     CDS             1163..2533
                     /locus_tag="VGA59_03975"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9718866.1"
                     /translation="MPFYEILSIALMVTFILALFTGYPVAWLLSGISLLFATIAITLT
                     TQFGVDTFLMTSWVKFSGIIDRFDAIMSNWVLVSLPMFVYMGLMLDRSGVADIMMRNF
                     VKVFGGIRGGLGLTVIVIGILLAASTGIVGASVVLLAMLSMPIMLQHQYSKAIASGIV
                     AASGTLGILIPPSIMLVLMADQVSVSVGDLFMGALVPGVLLGVLYMIFVMAVGIFRPD
                     LVPPRHADAEKLTLRQVGIAFLSTIPTFGLILVVLGSIFFGIATPTEASGLGAFGAML
                     LAAANRKLSWSVIRQVGIDTTLTTCFIFAIFIGATVFAYVLRMIGGDELITEFFKGTG
                     LGPTGLVLLVLAVVFVAGFFLDWFEIVLIILPLLAPVIKLSGIDMTWFLILVALMLQT
                     SFLTPPVGFSLFYLKGVVPKGVTIKDIYVGVIPFVLLQLAMVGLCLWFPQIILWLPKS
                     MYGSGG"
     gene            2724..3491
                     /locus_tag="VGA59_03980"
     CDS             2724..3491
                     /locus_tag="VGA59_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9718867.1"
                     /translation="MNELFSLAGRTALVTGGSRGIGRMIAKGFLSQGARVYISSRKAA
                     ACEDTARELSSLGPCFALPADVSTVEGARGLAAAYASKESALDILVNNAGAAWGESFD
                     TFPEKGWDKVMDLNLKSPFFLTQALHEALRTAGKARPAKVINIASIDGVSVNPLETYS
                     YAASKAGLIHLTKRMALRLVQDNIVVSGIAPGAFATEMNRTARDHGDEVARHIPAGRI
                     GTEEDMAGVAIYLASRAGDYVVGQTIVVDGGVTNARG"
     gene            complement(3617..4513)
                     /locus_tag="VGA59_03985"
     CDS             complement(3617..4513)
                     /locus_tag="VGA59_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX9718868.1"
                     /translation="MAVVICGSLAFDTIMSFEGRFAEQILPDQLHILNVSFLVPALRR
                     DFGGCAGNIAYSLKLLGGQPLPMATVGSDGYGYVARMHSLGISTEFVREVDETYTAQA
                     MIMTDRDNNQITAFHPGAMMHAHVTRIEARADIRLGIISPDGRDAMIEHAQQFKLAGI
                     PFVFDPGQGLPMFDGRELAAFVDASTWVTVNDYEGKMLSERTGWSSAEISRRVQGLVV
                     TLGGEGCEVWVDGEKTLVPPVKPSDIVDPTGCGDAWRGALLYGLEQGWPLLRCAQLGN
                     QVGAIKIASRGPQNYTLMSLRA"
     gene            complement(4533..5528)
                     /locus_tag="VGA59_03990"
     CDS             complement(4533..5528)
                     /locus_tag="VGA59_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HEX9718869.1"
                     /translation="MSLITRCPACGTLFKVVPDQLRISEGWVRCGHCAEVFDASAHLQ
                     GDAAAAGSDADPHAPRPANAPTAPAPPGAATVESEAFSSSFNTEIGEGVAFDDPDSTQ
                     IEAEALALREHPLDQPFELRRRDESEDEPSRPSGAEWAPDADTDSEDDREGDAGLHEV
                     SFVRKAGRNERWSGPRVRAALALAVLALAMLLALQIAVHDRDRLAAAAPALRPWLASL
                     CGPLNCRVGPPRHIDAIAIDSSSFNKLRGDAYRLNVTLRNQAGVEVAMPALELTLTDG
                     QDQALVRRVLMPSELGPGASVIAAASEWSGSLALAVAANGAGARIAGYRLLAFYP"
     gene            complement(5568..6449)
                     /gene="prmA"
                     /locus_tag="VGA59_03995"
     CDS             complement(5568..6449)
                     /gene="prmA"
                     /locus_tag="VGA59_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX9718870.1"
                     /translation="MFELGLLCPEDRVETVSEALDALDALSVSVEDADARTDAEQALF
                     GEPGMPAPKEGWQRSRVMALFATEAAAQEASRLLQVQDFFAGCKLLGITPVPDQDWVR
                     LTQSQFAPVEITPEFWIVPTWHEPPGQARQVIRLDPGLAFGTGTHPTTRMCLRWIAAH
                     PPLGQRVLDYGCGSGILAIGAARFGASQVDAVDIDPAAVASTLANARLNGLQINAGLP
                     DQAGGQYELVLANILATPLKILAPLLSSLVAPGGFLVLAGILERQAEELEAAYAPHGC
                     LRVSDSEDGWVLMTAAF"
     gene            complement(6478..7827)
                     /gene="accC"
                     /locus_tag="VGA59_04000"
     CDS             complement(6478..7827)
                     /gene="accC"
                     /locus_tag="VGA59_04000"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9718871.1"
                     /translation="MFKKILVANRGEIALRIQRACRELGVKAVMVYSEADREAKYVKL
                     AEEAVCIGPAPSAQSYLNMPAIISAAEVTDAEAIHPGYGFLSENADFAERVEKSGFQF
                     IGPTPESIRIMGDKVSAKQAMIKAGVPCVPGSEGELPDDPVQIRRMARSVGYPVIIKA
                     AGGGGGRGMRVVHTEAALINAVQMTKAEAGAAFGNPAVYLEKYLQNPRHIEIQVLADK
                     HKNAVFLGERDCSMQRRHQKIIEEAPAPGIPRKLIDKIGERCVTACKKIGYRGAGTFE
                     FLYENGEFYFIEMNTRVQVEHPVTELISGVDVVKTQIMVAAGEKLPFSQRDIVLRGHA
                     IECRVNAEDPYKFVPSPGRITMWHAPGGPGVRVDSHVYTNYFVPPNYDSMIAKIIVHG
                     DTREQALARMRTALLETVVEGINTNIALHRELLVDAKFMDGGTNIHYLEEWLSHHKR"
     gene            complement(8016..8468)
                     /gene="accB"
                     /locus_tag="VGA59_04005"
     CDS             complement(8016..8468)
                     /gene="accB"
                     /locus_tag="VGA59_04005"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX9718872.1"
                     /translation="MDLRKLKTLIDLVSESNVSELEITEAEGKVRIVKGGGQAPPMPA
                     MPANAAGALAASGLAAAPAASVAVAEVPAGHVVKSPMVGTFYRSASPGAKPFVEIGSQ
                     VKEGETICIIEAMKILNEIEADKSGTISQMLCENGQAVEYGQPLFVIV"
     gene            complement(8616..9170)
                     /locus_tag="VGA59_04010"
     CDS             complement(8616..9170)
                     /locus_tag="VGA59_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9718873.1"
                     /translation="MNPNSSQPPLTVGRRRLLYAGVAGVAAIAGAGLAWWKWRPGTTA
                     QDAAAALWPLQFETPAGSTLAMGTLRGKPLLVNFWATWCPPCIEELPLLEAFYRENAS
                     KSWQVVGLAIDQPSAVRTFLQRTPVSFPVGLAGLGGSELSKALGNIAGALPYTVVISG
                     SGEVVQRRMGRVTAPDLAQWAQLG"
     gene            complement(9167..9760)
                     /locus_tag="VGA59_04015"
     CDS             complement(9167..9760)
                     /locus_tag="VGA59_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718874.1"
                     /translation="MDSTKAEIAAAAARMVVEEGLEYGPAKRRAVRQLGLNARSALPD
                     NEAVEDSVREYIGLFCADTQPAELAALRKLALTWMERLAQFRPYLGGSVWHGTATRLS
                     DIYIQLFCDDPKSAEMALIDKCVDYEARTITGFNGEPVEALSLSSVSRELGEAIGVHL
                     MIYDHDDVRGALKRDARGRIPRGDMAAVRALLDNGQA"
     gene            9826..10188
                     /locus_tag="VGA59_04020"
     CDS             9826..10188
                     /locus_tag="VGA59_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718875.1"
                     /translation="MRSTMMREPLSRNHLLERAGKLFAGNELASLVGGAAVPEALIRA
                     FDRHGIWILQGWVMTETSPVCTTAATSPGTAKASAKSRCAGLSSPAATTPSTRRSSPT
                     TAGLRTGDVACLDTHWAL"
     gene            10268..10483
                     /locus_tag="VGA59_04025"
     CDS             10268..10483
                     /locus_tag="VGA59_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718876.1"
                     /translation="MAHPAAAEAAVIAVPDEKWGERPWYSGQGKTQARANSTRTCFDT
                     VSRTGSCPSDTNSSTPCRARRPASSGR"
     gene            10536..12023
                     /gene="mpl"
                     /locus_tag="VGA59_04030"
     CDS             10536..12023
                     /gene="mpl"
                     /locus_tag="VGA59_04030"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX9718877.1"
                     /translation="MHIHILGICGTFMGGLAALARESGHEVTGCDAGVYPPMSEQLRA
                     LGIELVEGYGADQVALKPDVWVVGNVVSRARQADGTPRYPLMEAILDAGLPYTSGPQW
                     LAEQVLQGRHVLAVAGTHGKTTTAAMLAWVLEHAGLQPGFLVGGVPANFGISARLGRA
                     VPAAAAASPPSLFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFDDLAAI
                     ERQFHHLVRTVPSTGRVIVNGLEESLARVLHMGCWSELRSFGAAVSDFSAQGEPHSFD
                     VLERGRPVAHLEWELSGVHNQLNALAAIAAAQHVGVAPEAAARSLREFLNVKRRMEVR
                     GIVNGITIYDDFAHHPTAMRTTVDGLRRKVGACEIAPDDAAAGARILAVFEPRSNTMK
                     LGAMKAQLPWSLEQADLSFCHSGGLGWNAAEVLAPMGAKARVADTIDELVRQVTEAAR
                     PGDHILCMSNGGFGDVHAKLLHSLEKSSPEGLVTPEPEGFADSVD"
     gene            complement(12020..13027)
                     /locus_tag="VGA59_04035"
     CDS             complement(12020..13027)
                     /locus_tag="VGA59_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718878.1"
                     /translation="MTGFAPQQRAVLEASLVRPPGLPRWRIGAFGDADAWWVNGAKVR
                     VLPDGNLKVAAGLPTERALNLDLSDIDRPVAFAAPLASADFDPRYTFDPVSEPSIHAV
                     LLQFERWLWLVRAQFVLGAQIIRRGPELRHGIYHVSHRGKLLAVLDFQHGKAAIAPGL
                     YPVDLWQAQWDKRPIGAGGLPERFVHLTPGQLAWTYVRRTDRDVLPARYRTRTIYYRH
                     VPGVPLRWLRDSQLLLLRELSAEPGTLEALGRRTGLPVSQTEHDLACLYYAGSITTTG
                     PKAAAPVAARHDSQPNSVGPGFDSLLSTNGHADDQYGQTAPVLLEHKRMAPLADAAEN
                     S"
     gene            complement(13266..14270)
                     /locus_tag="VGA59_04040"
     CDS             complement(13266..14270)
                     /locus_tag="VGA59_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718879.1"
                     /translation="MELPLLRLGLAGFSAEQDDQLNLLLQQVSTSNWQIGRFADADAW
                     WVNGARVQMLLDSTIRIGSGEPGGRSLHLDLRGVDRPVAFALPLASPQFDPLCTFQAQ
                     SLPSVAAVLEKFEAWVRPLIAQFYLAARILEQESVLGPAVHHVTADGVLIGVVNMRGE
                     TGVLPTAGPIDFENAMWERRPASAGAIPEHFARSSLAQLMWQFAVRTNRDILPARYRT
                     GLLYFRRPPRLPPRLMTDSHLLLLRELACAPGDFTALQQRTGLGGQALARDLAALYFV
                     GAITSNPKRATRVPVRAAADSAHSHPNSVPSGMDAEPPAALPRLAFADLTAPAPIGTH
                     "
     gene            complement(14362..15486)
                     /locus_tag="VGA59_04045"
     CDS             complement(14362..15486)
                     /locus_tag="VGA59_04045"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX9718880.1"
                     /translation="MNAKATAASNTWYAHPVDKTSQTDDERIKDITVLPPPEHLIRFF
                     PIRGTDVESLITGTRRNIHNIMAAKDDRLLVVIGPCSIHDPGAALDYARRLMEARTRF
                     KVTLEIVMRVYFEKPRTTVGWKGLINDPYLDESFRIDEGLRIARQLLIEINRLGLPAG
                     SEFLDVISPQYIGDLISWGAIGARTTESQVHRELASGLSAPIGFKNGTDGNIRIATDA
                     IQAAARGHHFLSVHKNGQVAIVQTNGNRDCHVILRGGKTPNYDAPSVAAACKDLEAAR
                     LPTTLMVDCSHANSSKQHDRQIDVARDVAGQIAKGSHSVFGVMVESHLNAGAQKFTAG
                     KDDPATLEYGKSITDACLGWGESLQLLELLSEAVKLRRSR"
     gene            complement(15715..16269)
                     /locus_tag="VGA59_04050"
     CDS             complement(15715..16269)
                     /locus_tag="VGA59_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718881.1"
                     /translation="MTSNSRLSIDPSTHLPSIHTFGELGHFLRASVADEDSRQFSDSM
                     AELAVQIEAIVPSLRRNRVRSTIAPLLVDLLTVVRAHRNLVVNLGLAWRGLYEYAAYL
                     QALNNFRVLIGQWLLDGGPDEAELLLSSEDFELVAWRTLGEGMLLIDMYERLVSEPPV
                     QSTATAGNEPQVERAIQWWKKLRL"
     gene            complement(16304..17764)
                     /gene="tldD"
                     /locus_tag="VGA59_04055"
     CDS             complement(16304..17764)
                     /gene="tldD"
                     /locus_tag="VGA59_04055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX9718882.1"
                     /translation="MISREPTLERLATAQRLLLEPFGLDETHLLRALAEITSHRVDDA
                     DLYFQYTRSEGWSLEEGIVKTGSFSIDQGVGVRAVSGEKTAFAYSDDISEASLLDAAR
                     TVRTISALSRTGRAKVGAKKIAGGRSLYQGLDPIATLDSTAKVRLLERAEKLARSKDP
                     RVAQVMAGLASEYDVIMVARADGTLAADIRPLVRLSITVIAEQNGRREVGSGGGGGRF
                     GLAYFDDERIESYVNDAVNAALTNLDSRPAPAGEMTVVLGPGWPGILLHEAIGHGLEG
                     DFNRKGSSAFSGRVGQRVAAKGVTVLDDGTIADRRGSLNVDDEGNTSQRNVLIEDGIL
                     RGYLQDALNARLMGVKSTGNGRRESYAHIPMPRMTNTYMLGGTREPGEILASVKKGLY
                     ATNFGGGQVDITSGKFVFSASEAFWVENGKILYPVKGATIVGNGPDALTRVTMIGNDM
                     KLDTGVGTCGKEGQSVPVGVGQPTLRIDGLTVGGTA"
     gene            complement(17895..18773)
                     /locus_tag="VGA59_04060"
     CDS             complement(17895..18773)
                     /locus_tag="VGA59_04060"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9718883.1"
                     /translation="MKPAIGVVGVGNMGGAMAARLLEQDWPVRVCDIDGAKVRALQAL
                     GAEGRADAAQAAAGCAALIVCVVDAAQTREVLFGRGGAADAMEPGQAVLLCPTIAPRD
                     VEDFARHLAGRGIAAIDAPMSGGPARARDGSMSLMIACEDAAFERHRALIGALSSKVF
                     RIGSRPGDAARTKLVNNLLAGINLAGAAEAMAMAQRLGLDLGRTLDVIEQSSGQSWIG
                     SDRMRRAIAGDYEPRAHVTLLEKDTRLAVEAAAATGFTGPLGIAARDAFARAAQAGLA
                     DLDDAALFKLLSQRPD"
     gene            complement(18781..>19567)
                     /locus_tag="VGA59_04065"
     CDS             complement(18781..>19567)
                     /locus_tag="VGA59_04065"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX9718884.1"
                     /translation="TPLMLAWWFQKREGQLRALDFLVAGVLLAIPVGLIVKQPDLGTS
                     VLVLAAGLSVIFFAGLSWKLILPPVVLGLTAAILLIVFEPQVCAEGVRWPVLHDYQQQ
                     RICTLLDPRKDPLGKGFHIIQGMIAIGSGGVLGKGFMQGTQTHLEFIPERTTDFIFAA
                     FSEEFGLLGNLFLIAGFVFLVLRGLAIALEASTVFSRLLAGAVTMIFFTYAFVNMGMV
                     SGIVPVVGVPLPFISYGGTAMATLGMGLGILMSISKAKRLVQS"
BASE COUNT         3262 a         6485 c         6343 g         3477 t
ORIGIN      
        1 ctgttccagg cgctggaaaa gggcgtgctc gacggcaccg agtttccgct gccaacggtg
       61 gatgaacagc tcggcttttc caaggttgcc aagaattact acatgcccgg ctggcaccaa
      121 ccgtccacga accagttcct gtacgtgaac atggctgcct ggaccaagct caagccgcaa
      181 accaaggccc agatcgagac cacctgcacc gccggcgtga cgatggcaat cgccaaggcg
      241 gaagcgctgc agggtcaaac cttgaccaag ttcgaaaagg acggcgtcaa ggcccgacag
      301 ttcaataagg tcatgctgga tgcgttcgcc aaggcgacaa aggaagtcat ggctgaagag
      361 tcggccaaag accctctgtt caagaaggtt tacgatagca tggtcgcgtt ccagcaaaag
      421 aatcaggctt ggcaccacct cggctacctg ccgcgtgacc ataagtaatc gctgaatcgc
      481 acgacaataa gaagcgtctc tcaagccgtt ccggggagca cgagctcccc ggaacggctc
      541 attttgcgcc aggagaagag tgatggaatc gcatttgcat gaggtcgaag agctgatcgc
      601 cggacattcc ggcatcgggc tgccggtcac cggtttttcg catgcggtcg accgggtgat
      661 cgaatgggtc ggcgaggggg cttccatcct ttggacgatc ctcgtcctgg tcatcgtctt
      721 ccaggtcttt ctgcgctacg ccctggggct gggctccatc atgctcgaag aaacccaatg
      781 gcatctgtat gccgtcggct tcctgctcgg actttcattc accgaaattc gcgagcgcaa
      841 cgttcgcatc gatggcctgg ccgagggctt gcagcgaagg acgcggctgt ggattgaact
      901 catcggcatc gccttcttcc tgctcagttt ttcgctgctc gtgatctggt tcgccgtgcc
      961 ctacgtgatc acttcgtggg agctgagcga ggtgtcggcg gcgccgggcg ggttgcctta
     1021 tcgctgggcg ctgaagtcct tcctgatcac ggcattcgca ctgctcgcgc ttgcgggggc
     1081 cagcaggctc acgcgggtat gggccgccct gttcggacaa cgggccatcc cctcgtaacg
     1141 caaaccttcc ctggcgccgc tcatgccttt ctacgaaatc ctctccatag cgctgatggt
     1201 gaccttcatc ctcgcgctgt ttaccgggta cccggttgcc tggctgctga gcggcatctc
     1261 cctgctgttc gccacgatcg caatcaccct gaccacccag ttcggggtcg acacattcct
     1321 gatgaccagc tgggtcaagt tctccggaat catcgaccgc ttcgacgcga tcatgtcgaa
     1381 ctgggtgctg gtatcgctgc cgatgttcgt ctacatgggc ctgatgctgg accgctcggg
     1441 cgtggccgac atcatgatgc gcaacttcgt caaggtgttc ggcggcattc gcggcgggct
     1501 gggcctgacg gtgatcgtga tcggcatcct gctcgccgct tcgaccggca tcgtcggcgc
     1561 ctcggtggtg ttgctggcca tgttgtcgat gcccatcatg ctgcagcatc aatattccaa
     1621 agccatagcc tccggcatcg ttgccgcgtc gggcacattg ggaatcctga tcccgccatc
     1681 catcatgctg gtgctgatgg ccgaccaggt ctcggtctcc gtcggcgacc tgttcatggg
     1741 agcgctggtc cccggcgtgt tgctgggcgt gctgtacatg atcttcgtga tggccgtcgg
     1801 catcttccgg cccgacctgg tgccgccgcg ccatgccgat gccgagaagc tgaccctgcg
     1861 gcaggtcggc atcgctttcc tgtccacgat tccgaccttc ggcctgattc tggtggtgct
     1921 gggctccatc ttcttcggga tcgccacgcc gaccgaagcc tccggtttgg gcgctttcgg
     1981 cgcaatgctg ctcgcagccg ccaacagaaa actcagctgg tcggtgatac gccaggtcgg
     2041 gatagacacc acgcttacca cctgcttcat ctttgccatc ttcataggcg caacggtgtt
     2101 tgcctatgtt ctgcgcatga tcggtggcga tgaactgatc accgagtttt tcaaggggac
     2161 gggattgggg cccacggggc tggtgttgct cgtgctggcg gtcgtttttg tcgccggctt
     2221 ctttctcgac tggttcgaga tcgtgctgat catcctgccc ctgctggcgc cagtcatcaa
     2281 gctgagcggg atcgacatga cctggttcct gattcttgtc gcgttgatgc tgcagacctc
     2341 gttcctgacg ccgcccgtcg gtttctcgct tttctatctg aagggcgtgg tgccgaaggg
     2401 ggtcacgatc aaggacatct acgtcggcgt catccccttc gtcctgctgc agctcgcgat
     2461 ggtggggctg tgtctgtggt tcccgcagat catcctgtgg ctcccgaaaa gcatgtacgg
     2521 ctcaggcggt tgaccttgcg agggcgccga taacccgggc ggccgcgaac tgcggcgctg
     2581 gagcggactc cggacccgcg ccaggaacat tccggtgctg gcggggagcg gcagggatgc
     2641 ccgtcctggg cgccgtgtca tccaggtgat tggcctggcc cgcgactcgc gctaacctgc
     2701 gcggtttcca gcaaggaacc gcaatgaacg aactcttttc cctggcgggc cgcacggccc
     2761 tggtgacggg cggctcgcga ggcatcggcc gcatgatcgc caagggcttc ctcagccagg
     2821 gcgcccgcgt ctacatctcc tcgcgcaagg ctgcggcctg cgaagacacg gcccgggagc
     2881 tttcgtccct tggcccatgc tttgccctgc cggccgatgt gtccacggtc gagggcgccc
     2941 ggggcctcgc ggccgcctat gcgtctaagg aaagcgcgct cgacatcctg gtgaacaacg
     3001 cgggcgcggc ctggggcgaa tccttcgaca ccttcccgga aaagggctgg gacaaggtga
     3061 tggacctgaa cctgaagtcg cccttcttcc tcacgcaagc gctgcacgag gcgctgcgca
     3121 cggcgggcaa agcccggccg gccaaggtga tcaacatcgc ctcgatcgac ggtgtttcgg
     3181 tcaatccgct ggagacctat tcgtacgcgg ccagcaaggc cggcctgatc cacctcacga
     3241 agcgaatggc gctgcggctg gtccaggaca acatcgtggt gagcggcatc gcgccaggcg
     3301 cgtttgccac cgagatgaac cgcacggcgc gcgaccacgg cgatgaggtc gcacgccaca
     3361 ttccagcagg ccgcatcggc accgaggagg acatggccgg ggtggccatc tacctggctt
     3421 cgcgcgccgg agattacgtg gtgggtcaga ccatcgtggt cgacggcggc gtgaccaacg
     3481 cgcggggttg acgaggctgt catcccgggc tcgacccgcc gctgtccgga gatttgatga
     3541 gagttgtcat gccgggcttg acccggcatc catgtcactg gaccggcgct ctgcgtgagc
     3601 cactatggat tgcgggtcaa gcccgcaatg acatcagcgt gtagttctgc ggcccgcggc
     3661 tggcgatctt gatcgcgccg acctggttgc ccagttgcgc gcaacgcaac agcggccaac
     3721 cctgttccag tccatagagc agcgcgccgc gccacgcgtc cccacaaccc gtgggatcga
     3781 cgatgtcgct cggtttgacc gggggcacaa gcgttttttc gccgtcgacc catacttcgc
     3841 agccctcgcc gcccagcgtc accaccaggc cctggacgcg ccgcgagatc tcggcgctgc
     3901 tccagccggt ccgctcgctg agcattttcc cttcgtagtc gttcaccgtg acccacgtgg
     3961 acgcgtcgac gaatgcagcg agttccctgc cgtcgaacat cggcagaccc tggcccgggt
     4021 cgaacacgaa ggggatgccc gccagcttga actgctgcgc atgctcgatc atcgcgtcgc
     4081 gcccgtccgg cgagatgatg cccagcctga tgtcggcccg tgcctcgatg cgcgtgacgt
     4141 gcgcgtgcat catggcgccg gggtggaaag cggtgatctg gttgttgtcc cggtcggtca
     4201 tgatcatggc ctgcgcggtg taggtctcgt ccacttcacg cacgaattcg gtgctgattc
     4261 ccaggctgtg catgcgtgcc acgtagccgt agccgtcgct gcccaccgtg gccatcggca
     4321 gcggctggcc gcccagcagc ttgaggctgt acgcgatgtt cccggcgcag ccgccgaagt
     4381 cgcgccgcaa ggcaggcacc aggaacgaga cgttgaggat gtgcagctgg tcgggcagga
     4441 tctgctcggc gaagcgccct tcgaaactca tgatggtgtc gaacgccaga gagccacaaa
     4501 tcacaacggc catgaatgat ctcgggtgaa ggtcagggat agaaagccag cagccggtaa
     4561 cccgcgatgc gggcgcccgc gccattggcg gcaacggcca gcgccagcga gccggaccat
     4621 tcggacgctg ccgcgatcac gctcgcgcca ggcccgagtt cagacggcat gaggacccgg
     4681 cgcaccaggg cctggtcctg gccgtcggtg agcgtcagtt ccagcgccgg catcgccact
     4741 tcgacgccgg cctggtttct cagggtgacg ttgagccggt aggcgtcgcc gcgcagcttg
     4801 ttgaacgatg aactgtcgat ggcgatcgca tcgatgtggc gcggcggccc gaccctgcag
     4861 ttcagcggcc cgcacaggct tgccagccac gggcgcagcg cgggcgcggc cgcggccagt
     4921 cgatcgcggt catggacggc gatctgcagt gccaggagca tggccagcgc gagcaccgcg
     4981 agcgccagcg cggcccgcac gcgtgggccg gaccagcgct cgtttcgccc ggccttgcgg
     5041 acgaaggaaa cctcgtgcag cccggcgtcg ccctcccggt cgtcctcgga atcggtatcc
     5101 gcgtccggcg cccactcggc tcctgatgga cgcgacggct cgtcctcgct ttcatcgcgc
     5161 cgccgaagct cgaacggctg atcgagcggg tgctccctca gcgccagcgc ttcggcctcg
     5221 atctgggtcg aatccgggtc gtcaaaggca acaccttcgc cgatttcagt gttgaacgaa
     5281 gacgaaaaag cctcggactc gacggtagcc gcccccgggg gcgcgggcgc ggttggcgca
     5341 ttcgccggac gaggtgcgtg ggggtccgcg tcgcttccgg ccgcggccgc gtcgccctgc
     5401 aggtgcgcgc tggcgtcgaa tacttcggca cagtggccgc agcgcaccca gccctccgag
     5461 atcctgagct ggtcgggaac gactttgaac agggtcccgc aagcggggca gcgcgtgatc
     5521 aggctcatca gccggtgatt gtagccagcg cctggcgccg tccgcggcta gaaagcggcc
     5581 gtcatcagca cccagccatc ctcggagtcg ctcacccgca ggcagccatg gggtgcataa
     5641 gccgcctcga gttcctccgc ctggcgctcc aggatgccgg ccagcaccag gaagccgccc
     5701 ggcgccacca gcgaactcag cagaggtgcc agtatcttca gcggcgtggc caggatattc
     5761 gccagcacca gctcatattg cccgccggcc tggtccggca agccggcgtt gatttgcagg
     5821 ccattgagcc gcgcgttggc cagggtggat gccaccgcgg ccggatcgat gtccaccgcg
     5881 tccacttgcg aggcgccgaa gcgcgccgca ccgatggcca gaatcccgga tccgcagccg
     5941 taatcgagca cgcgctggcc caacggagga tgcgcggcga tccagcgcag gcacatccgg
     6001 gtggtcgggt gggtgcccgt gccgaaggcc aggccggggt cgagacggat cacctgacgc
     6061 gcctggccgg gcggctcgtg ccaggtcggc acgatccaga attccggcgt gatttccacc
     6121 ggcgcaaact gggattgggt cagccgcacc caatcctgat cgggcaccgg cgtgatgccc
     6181 agcagcttgc agccggcaaa gaagtcctgc acctgcagca ggcgcgacgc ttcctgcgcg
     6241 gctgcctcgg tggcgaacag cgccatcacg cgcgagcgct gccagccctc cttgggcgcc
     6301 ggcatgccgg gttcgccgaa gagtgcctgc tcggcgtcgg tccgcgcatc ggcgtcctcc
     6361 accgacacgc tcagcgcgtc gagcgcatcc agcgcttcgc tgaccgtctc gacgcggtcc
     6421 tcgggacaaa gcaggcccag ttcgaacatc atggggagca gccgcgccga gcgctggcta
     6481 gcgcttgtgg tgcgacagcc actcttccag gtaatggatg ttggtgccac cgtccatgaa
     6541 cttggcgtcc accagcaact cccggtgcag ggcgatgttg gtgttgatcc cttcgaccac
     6601 ggtctccagc agcgccgtgc gcatgcgcgc cagcgcctgc tcccgggtgt ccccgtgcac
     6661 gatgatcttg gcgatcatcg aatcgtagtt gggcggcacg aaatagttgg tgtacacgtg
     6721 cgaatcgacg cgcacgcccg ggccgcccgg cgcatgccac atggtgatgc gccccggcga
     6781 gggcacgaac ttgtacggat cctcggcgtt cacccggcat tcgatggcat ggccacgcag
     6841 cacgatatcg cgctggctga aaggcagctt ctcgccggcg gccaccatga tctgggtctt
     6901 gaccacgtcg acgccgctga tcagttcggt gaccgggtgc tcgacctgca cccgcgtgtt
     6961 catctcgatg aaatagaact cgccgttctc gtacaggaat tcgaaagtgc cggcgccccg
     7021 gtagccgatc ttcttgcagg cggtcacgca gcgctccccg atcttgtcga tcagcttgcg
     7081 cgggatgccc ggcgccggcg cctcttcgat gattttctgg tggcggcgtt gcatggaaca
     7141 gtcgcgctcg cccaggaaga ccgcgttctt gtgcttgtcg gccaggacct ggatctcgat
     7201 gtggcgcggg ttctgcaggt acttctcgag atagaccgcg gggttgccga acgcggcgcc
     7261 ggcctcggcc ttggtcatct gcacggcatt gatcagcgcc gcctcggtgt gaaccaccct
     7321 catcccgcgc ccgccgccgc cgccggccgc cttgatgatc acgggatagc ccacagaccg
     7381 ggccatgcgg cggatctgca ccgggtcatc cggcaattcg ccttccgagc cgggcacgca
     7441 gggcaccccc gccttgatca tggcctgctt ggccgagacc ttgtcgccca tgatgcggat
     7501 ggattcgggc gtgggcccga tgaactggaa gccgcttttt tccacccgct cggcaaagtc
     7561 ggcattctcg gacaggaagc cgtagccggg gtggatggct tccgcgtccg tcacttcggc
     7621 cgctgaaatg atggcgggca tgttgaggta gctctgggcc gaaggcgccg gccctatgca
     7681 caccgcctcc tcggccagct tcacgtattt cgcttcccgg tcggcctcgg aatacaccat
     7741 aaccgccttg accccgagtt cgcggcaggc gcgctggatc ctcagggcga tttcgccgcg
     7801 attggccact agtatttttt tgaacattgt tgtttcacgg ctcaatcgcc ctcagcgggc
     7861 tgcgcccgcc ccgcgatttg gtgtgcgcat cacatgctgc gcatatgagc gcacccccgc
     7921 gcctgcggcg cgaacgcccc taagcatgtc tcacgcgccc aaggcgcgac cgccgcggtt
     7981 ggcaataaac attcttcctg gacatgaacc ggcgctcaga caatcacgaa cagcggttgc
     8041 ccgtactcca cggcctggcc gttctcgcac agcatctggc tgatggtgcc gctcttgtcg
     8101 gcctcgatct cgttgagtat cttcatcgcc tcgatgatgc agatcgtttc gccttccttg
     8161 acctggctgc cgatttccac gaagggtttg gcccccgggc tggccgagcg atagaaggtg
     8221 ccgaccatcg gcgacttgac gacgtggccg gccggaactt cggccacggc gaccgatgcg
     8281 gcgggcgcgg ccgcgagtcc gctcgccgca agcgcccctg ctgcattggc cggcatcgca
     8341 ggcatcgggg gggcttggcc gccgcccttg acgatacgca ccttgccttc cgcctcggta
     8401 atctccagtt cggaaacatt cgattcggac acgaggtcga tcaacgtctt cagtttgcgc
     8461 aaatccatgt gctctccaag gcccgaacga agggaagagg aatttacagc aaaattcgct
     8521 tacttcccgt ttcgtcgccg atttttagtt tattttcagt aaatgacttg acagcgcggc
     8581 gcggttcggc gctggcgcga gcgcatgcga ggcctctatc ccagctgggc ccactgggcc
     8641 aggtcggggg ctgtgacccg tcccattcta cgttgcacga cttccccgga tcccgagatc
     8701 accaccgtat agggcagggc gcccgcgata ttgcccaggg ctttggacag ctcggagcct
     8761 cccaagccgg ccaatcctac cggaaagctg accggtgtcc gttgcaggaa agtgcgcacg
     8821 gcgctgggct ggtctatggc gagaccaacg acttgccagc ttttagatgc attttccctg
     8881 tagaaagctt ccagaagcgg caactcctcg atgcacggcg gacaccaggt ggcccagaag
     8941 ttgaccagca ggggtttgcc gcgcagcgtc cccattgcca gtgtcgaccc cgcgggcgtc
     9001 tcgaactgca gcggccaaag cgccgcagcg gcgtcttgcg cggtcgtccc gggccgccat
     9061 ttccaccacg ccaggcccgc tccggcaatc gcggcaacgc cggcgacgcc cgcatagagc
     9121 agacggcgcc ggccgacggt caaaggtggc tgcgagctat tcgggttcat gcctgcccat
     9181 tgtccagcaa ggcgcgcaca gccgccatgt cgccgcgcgg gatgcgaccc ctggcgtccc
     9241 gcttgagtgc gccgcgcacg tcgtcgtggt cgtagatcat caaatgcaca cctatggcct
     9301 cgcccagctc gcggctcacg ctcgataggc tcagcgcttc caccggttcc ccgttgaagc
     9361 ccgtaatggt acgagcctcg tagtcgacgc acttgtctat cagcgccatc tcggccgatt
     9421 tggggtcgtc gcaaaacagc tggatgtaga tatccgacag ccgggtagcc gtgccgtgcc
     9481 acacagagcc gcccaggtag gggcgaaact gcgccagccg ttccatccag gtcagcgcca
     9541 gcttgcgcag cgccgcgagc tcagccggtt gcgtatcggc gcaaaacagt ccgatgtact
     9601 cgcgaaccga gtcttccacg gcttcgttgt ccggcaaagc gctgcgggcg ttcaacccga
     9661 gctgcctgac cgcgcgccgc ttggcgggcc cgtattcgag cccctcttcc accaccatgc
     9721 gcgccgccgc cgccgcaatt tccgctttcg tgctgtccat gcgggacatt ctgcattgat
     9781 tgggaatggc cgggccctta gagtgatttc aaccccggag gcaccatgcg gagcaccatg
     9841 atgagagagc cgctctcgcg caaccacctg ctcgaacgtg caggcaagct gttcgcaggg
     9901 aacgaactcg cctccctcgt tggcggcgcg gccgtgccgg aggcgctgat ccgcgcgttc
     9961 gaccggcatg gaatctggat ccttcaaggc tgggtcatga cggaaacctc gccggtatgc
    10021 acgacggcgg cgaccagccc tgggacggca aaagcgtcgg cgaaatccag gtgcgcgggc
    10081 ctttcatcac cggcagctac cacgccgtcg acccggagaa gttcaccgac gacggctggg
    10141 ctgcgcaccg gggatgtggc gtgcctggac acgcactggg ctttgtgaag atcacggatc
    10201 ggaccaagga cctgatcaag tccggtggca agtggatcac agttccgtgg gcctggagaa
    10261 tgccatcatg gctcaccccg cggcggcgga agccgcggtg atcgctgtcc ccgatgaaaa
    10321 gtggggcgag cggccgtggt actcaggcca gggcaaaacg caagcgcggg ccaattcaac
    10381 gcgcacctgc ttcgacacgg tttcgcgaac tggcagttgc cccagcgata cgaattcatc
    10441 gacgccgtgc cgcgcacgtc gaccggcaag ttctggaaga tgaaactgcg cgagcgcttc
    10501 ccgaaatagg ctcggagcgc cccgataatc gacgcatgca cattcacatc ctcggcattt
    10561 gcggcacatt catggggggg ctggccgcac tcgcgcgcga gtccggccac gaggtcaccg
    10621 gctgcgacgc gggagtctat ccgcccatga gcgagcagct ccgggccctg ggcatcgagc
    10681 tcgtcgaagg ttacggcgcc gaccaggtcg ccctcaagcc cgacgtctgg gtcgtgggca
    10741 acgtggtctc acgcgcgcgg caggccgacg ggacgccgcg ctacccgctg atggaagcca
    10801 tcctcgatgc gggcctgccc tataccagcg gcccgcagtg gctggccgag caggtcttgc
    10861 agggccggca cgtgctggcc gtggccggca cgcacggcaa gacgacgacc gccgcgatgc
    10921 tggcgtgggt gctggaacac gcgggcctgc agcccggctt cctggtcggc ggcgtaccgg
    10981 ccaacttcgg aatttcggcg cggctcggcc gagccgtccc tgccgctgcg gctgcctcgc
    11041 cgccgtcgct cttcgtcatc gaggccgacg aatacgacac cgcgttcttc gacaagcgca
    11101 gcaagttcgt tcactaccgt ccgcgcaccg ccgtcctgaa caacctggag ttcgaccatg
    11161 cggacatctt cgacgacctg gcggccatcg agcggcagtt ccatcacctg gtgcgcactg
    11221 tgccttccac cggccgcgtc attgtcaacg gcctggaaga gagcctggcg cgcgtgctgc
    11281 acatgggctg ctggagcgag ctgcgcagct tcggcgccgc ggtgagcgac ttctcggccc
    11341 agggcgaacc ccatagtttc gatgtgctcg aacggggccg accggttgca cacctcgaat
    11401 gggaactctc cggcgtgcac aaccagctca atgcacttgc agcgatcgcg gctgcgcaac
    11461 acgtgggcgt cgcgccggaa gccgcggcgc ggtccctgcg cgagttcctg aacgtcaagc
    11521 gccgcatgga agtgcgcggc atcgtgaatg gcatcacgat ttacgacgat tttgcgcacc
    11581 acccgaccgc gatgcgcacc acggtggacg gactgcggcg caaggtcggc gcctgcgaaa
    11641 tcgcgcctga tgacgccgca gccggtgccc ggatcctggc ggtattcgag ccgcgcagca
    11701 acaccatgaa actgggcgcg atgaaggcgc agctcccctg gagcctggag caggccgacc
    11761 tgtcgttctg ccacagcggc ggcctcggct ggaacgcggc cgaagtgctg gccccgatgg
    11821 gcgccaaggc gcgggtggcc gataccatcg acgaactggt gcgccaggtc accgaggcgg
    11881 cgagaccggg cgaccacatc ctgtgcatga gcaatggtgg cttcggcgac gtgcacgcca
    11941 agctcttgca ttcactggaa aagtcctcgc ccgaagggct tgtcacgccg gagccggaag
    12001 ggtttgctga ctcagtggac taagagttct ctgccgcgtc cgccagcggc gccatgcgct
    12061 tgtgctccag caggactggc gcggtctgac cgtactggtc gtccgcatgc ccgtttgtgc
    12121 tcagtagcga gtcgaatcct ggcccgacgg aattcggctg gctgtcgtgc cgcgccgcca
    12181 caggcgcggc cgccttgggc cccgtcgtcg taatcgagcc cgcgtagtag aggcaggcca
    12241 ggtcgtgttc cgtttgactg accggcaagc cggtacgccg gcccaaggcc tccagcgtac
    12301 ccggttcagc cgacagctct cgcagcaaca gcagttgcga atcgcgcagc cagcgtagcg
    12361 gcacaccggg cacatgacgg tagtaaattg tccgggtgcg gtagcgtgcc ggcagcacgt
    12421 cgcggtcggt gcggcgcaca taagtccagg ccagctggcc tggagtcaga tggacaaatc
    12481 tttccggcag gccgcctgcg cctatgggcc gcttgtccca ttgggcctgc cacaggtcca
    12541 cgggatacaa cccgggcgcg atagcggcct tgccgtgctg gaagtccagc acggcaagca
    12601 gcttgcccct gtggctcaca tggtagatgc cgtggcgcaa ttccgggccg cgccggatga
    12661 tttgggcgcc caggacgaac tgggcgcgca ccagccacag ccagcgttcg aactgcagga
    12721 gcacggcgtg gatgctgggt tcggataccg ggtcgaacgt gtatcgcgga tcgaagtccg
    12781 cgctggccag gggcgcggca aaagccaccg gcctgtcgat gtcgctcaag tcgaggttca
    12841 gggcacgctc cgtcggcagg ccggctgcga ctttcaggtt gccgtcgggc aagacgcgga
    12901 ccttggctcc gttcacccac cacgcgtcgg catcgccgaa tgcgccgatg cgccagcggg
    12961 gcagacccgg gggccgtacc agcgaggctt cgagaaccgc cctttgctgc ggagcaaaac
    13021 cggtcatgcc cagccaaagg atgggctgct ctgccgcatc gtttgcgtgc gtcgctgggg
    13081 gcatagcggg tacttccttc tttcttgtgt cctgcccagt aactcagggc ttcgacacgt
    13141 tcattttgta acaaagtcgg acgcttgtcg tcccccattt gtgggatggc agcagtgaat
    13201 ggccgggacg gcgccgcggc cgccgctgca gcagtcgcgg cgctcgccac tcgccgccgg
    13261 ctagcctagt gcgtgcctat gggtgccggc gcggtcaggt cggcaaaggc caggcgcggc
    13321 agggccgccg gcggttccgc gtccatgccc gaggggacgc tatttgggtg gctgtgggcc
    13381 gaatcggcag cggctcggac cggcacgcgc gtggcgcgct tggggttgct cgtgatggcg
    13441 ccgacgaagt aaagcgcggc cagatcccgc gccagcgctt ggccccccag cccggtacgc
    13501 tgctgcaagg ccgtgaagtc gcccggagcg cacgccagtt cccggagcag caacaagtgc
    13561 gaatcggtca tcagccgcgg cggcagccgc ggcggccgcc ggaaatacag caagcccgtc
    13621 cggtagcgcg caggcaggat gtcgcggttg gtccgcaccg caaattgcca catcagctgc
    13681 gccaggctgc tgcgcgcgaa atgctcgggg atcgcgccgg ccgacgcggg acggcgctcc
    13741 cacatcgcgt tttcgaagtc gatcggaccg gcggtgggca gcacgccggt ttcgccccgc
    13801 atgttgacca cgccgatcaa caccccgtcc gcggtgacat ggtggacggc ggggccgagc
    13861 acactttcct gctcgaggat tcgcgcagcc agatagaact gggcgatcaa tggcctcacc
    13921 caggcttcga atttttccag gacggcggca acgctcggca gggattgcgc ctggaacgtg
    13981 cacaaggggt cgaactgcgg cgacgccagc gggagcgcaa aggccacggg acggtcaacg
    14041 ccccgcaggt ccagatgcag cgaccggcct cccggctcgc cggagccgat ccggatcgtg
    14101 ctgtcgagca acatctgtac gcgggccccg ttgacccacc acgcatcggc gtcggcaaac
    14161 cttccgattt gccaattgct cgtcgacacc tgttgcagca ggagattgag ttggtcgtcc
    14221 tgctcggcgg aaaacccggc aagccccaag cgtaacaagg gaagttccat gaatcttgcc
    14281 tccgcgccgg ctgaagaccg ggcgcacgga ggcaaatata gacgccttgt tactttatgt
    14341 aaacagtgcg ttacatattt ctcaccgcga tcgccgcagc ttgacagcct ccgacaaaag
    14401 ttccaacagc tgaagcgatt caccccagcc caggcaggca tcggtgatgc tcttgccata
    14461 ttcgagggtc gccggatcat ccttgccggc cgtgaacttc tgcgcgcccg cattcaggtg
    14521 gctttccacc atcacgccga acacggagtg cgaacctttg gcgatctgtc ccgcgacgtc
    14581 gcgcgccaca tcgatctgcc tgtcatgctg cttgctgctg ttggcatggc tgcagtcgac
    14641 catcagcgtc gtcggcagcc tggccgcttc caggtccttg caggcggccg ccacgctggg
    14701 ggcgtcgtaa ttgggcgtct tgccgccgcg caggatcacg tggcaatccc ggttgccgtt
    14761 ggtctgcacg atggcgacct gcccgttctt gtgcaccgac aggaaatggt ggccccgcgc
    14821 ggccgcctgg atcgcgtcgg tggcgatacg gatgttgccg tccgtgccgt tcttgaaacc
    14881 gatcggcgcc gacaggcccg acgccagttc ccggtgcacc tggctctcgg tggtgcgggc
    14941 cccgatcgcg ccccaggaaa tcaggtcgcc gatgtactgc ggggaaatca cgtccaggaa
    15001 ttcactgccc gccggcagcc ccagccggtt gatttcgatc agcagctggc gcgcgatacg
    15061 cagcccctcg tcgatgcgga agctctcgtc caggtagggg tcgttgatca ggcccttcca
    15121 gcccaccgtc gtgcggggct tctcgaaata gacgcgcatc acgatttcca gcgtgacctt
    15181 gaacctggtg cgtgcctcca tcagccggcg cgcatagtcc agcgccgcgc ccggatcgtg
    15241 gatcgaacag gggccgatga ccaccagcag ccggtcgtcc tttgcggcca tgatgttgtg
    15301 gatgttcctg cgggtaccgg tgatcagcga ttcgacgtcc gtgccgcgga tcgggaaaaa
    15361 ccggatcagg tgttcgggag ggggcagcac ggtgatgtcc ttgatgcgtt cgtcgtcggt
    15421 ctggctggtc ttgtcgacgg gatgcgcata ccaggtattg ctggcggcgg tggctttggc
    15481 gttcattcgt gggctcctct cgggttcaaa agaatcggga caaaaaaaac cgccggggtg
    15541 tccggcggtt ttttggggag ttcgttcgcg ttgggtctgg gtacgctcag atctctctac
    15601 cgccgggggc gcttgagaac cagaagtagc caaaaaaata cgcgcgagtc gtatgcatga
    15661 ggtgcaatgt agcacattcg gccgggcatc cgaccgtgca gcgaagcgct tgtctcacag
    15721 ccgcaacttc ttccaccact ggattgcccg ctccacctgc ggctcattgc ctgcggtggc
    15781 ggtggattgg accggcggct cgctcaccaa ccgctcgtac atgtcgatca gcagcatgcc
    15841 ctcgcccagc gtcctccacg ccacgagctc gaagtcctcc gagctgagca acagctcggc
    15901 ttcatccggc ccgccgtcga gcagccattg gccgatcagg acgcggaaat tgttcaaggc
    15961 ctgcaagtag gcggcgtact cgtaaaggcc gcgccacgcc aggcccaggt tgaccaccag
    16021 gttgcgatga gcgcgcacca ccgtgagcaa gtcgaccagc aagggggcga tggtgctgcg
    16081 tacgcgattg cgccgcaggc tcggcacgat ggcttcgatt tgcaccgcga gctcagccat
    16141 gctgtccgag aattggcggc tgtcctcgtc ggccaccgag gcccgcagga aatggccgag
    16201 ctcgccgaat gtgtgaatgc ttggcaggtg cgtggacgga tcgatgctga ggcgcgaatt
    16261 ggaagtcatg tcgttgtggt tccttcgagg cgctgcggcg cgatcaagcc gtcccgccca
    16321 cggtcaggcc gtcgatgcga agggtcggct ggccgacgcc caccggcacg ctctggcctt
    16381 ccttgccgca cgtgcccacg ccggtgtcca gcttcatgtc gttgccgatc atggtgacgc
    16441 gcgtgagcgc gtccggacca ttgccgacga tggtcgcgcc cttgacgggg tacaggatct
    16501 tgccgttttc gacccagaat gcctcgctgg cggagaacac gaacttgccg gaggtgatgt
    16561 cgacctggcc gccgccgaag ttggtggcgt agaggccctt cttgacgctg gccaggatct
    16621 cccccggttc gcgcgtgccg ccaagcatgt aggtgttggt catgcgcggc atggggatat
    16681 gggcatagct ttcgcggcgg ccgttgccgg tggacttgac gcccatcagg cgggcattga
    16741 gcgcgtcctg gagatagccg cgcaggatgc cgtcctcgat cagcacgttg cgctggctgg
    16801 tgttgccttc gtcgtcgaca ttgagcgagc cgcgccggtc ggcgatcgtg ccgtcgtcca
    16861 gcacggtgac gcccttggcc gccacgcgct ggccgacgcg gccggaaaac gcgctcgagc
    16921 ccttgcggtt gaagtccccc tccaggccat ggccgatggc ttcgtgcaac aggatgccgg
    16981 gccagccggg gcccagcacc acggtcattt cgcccgccgg cgccggccgc gagtccaggt
    17041 tcgtcagcgc cgcgttcacg gcgtcgttca catacgactc gatgcgctcg tcgtcgaagt
    17101 aggccaggcc gaaacgcccg ccgccgccgc ccgaacccac ttcgcgccgc ccgttctgct
    17161 cggcgatcac ggtgatcgac agccgcacca gcggccgaat gtcggcggcc agcgtcccgt
    17221 cggcgcgggc gaccatgatc acgtcgtact cgctggccag cccggccatg acctgggcca
    17281 cacgcggatc cttggagcgc gcgagctttt ccgcccgctc cagcagccgg accttggcgg
    17341 tgctgtcgag cgtcgcgatc gggtcgagcc cctggtagag agaacgcccg cccgcgattt
    17401 tcttcgcgcc caccttggcg cggccggtgc gcgacagcgc cgagatcgtc cgaacggtgc
    17461 gggccgcgtc cagcagcgaa gcctcggaga tgtcgtcgga ataggcaaaa gccgttttct
    17521 cgccgctcac ggcgcgcacg cccacgccct ggtcgatgct gaagctgccg gtcttgacga
    17581 tgccctcttc caggctccag ccttcgctgc gggtgtactg gaaataaagg tcggcatcgt
    17641 ccacgcggtg cgaggtgatt tcggccagag cgcgcaacag atgggtttca tccaggccga
    17701 agggttcgag cagcaggcgc tgggccgtgg ccaggcgctc gagagtgggt tcgcgggaaa
    17761 tcatcggccc attctagacg tgcatcgccg gccctgcgcg ggtgtatcgg cgacgcgcag
    17821 gggctttatc ggtagcgcgg cggcgattcg ccggtccgct tcggtgacca ggacttgtgc
    17881 gtggcaaggc cgtcctaatc cggtcgctgc gacagcagct tgaacagtgc cgcatcgtcg
    17941 agatcggcca gcccggcctg ggcagcccgg gcaaatgcat cgcgggcggc aatacccagc
    18001 ggcccggtga agccggtggc ggccgcagcc tccaccgcca gccgggtatc cttttccaac
    18061 agggtcacat gcgcgcgcgg ttcatagtcg ccggcgatgg cgcggcgcat gcggtccgag
    18121 ccgatccagc tctggccgct ggactgctcg atcacatcga gcgtgcgtcc cagatccagt
    18181 cccaagcgct gcgccatcgc catcgcctcg gctgcccccg ccaggttgat gccggccagc
    18241 aggttgttga ccagcttggt tcgggcagcg tcgccgggac gcgaaccgat gcgaaagacc
    18301 ttgctgctca aggccccgat caaggcgcga tggcgttcga aggccgcgtc ctcgcaggcg
    18361 atcatcaggc tcatcgatcc gtcccgcgcg cgtgccggcc cgcccgacat cggcgcgtcg
    18421 atagcggcga tgccgcgccc cgccaagtgc cgggcgaaat cctcgacgtc ccgcggcgcg
    18481 atggtcgggc agagcagcac ggcctggccc ggctccatcg catccgcggc gccgccgcgg
    18541 ccgaacagca cttcccgggt ctgggccgcg tcaaccacgc acacgatcag cgccgcgcag
    18601 ccggcggcgg cttgcgcggc atcggctcgt ccctcggcgc ccagggcttg aagggcccgg
    18661 accttggcgc cgtcgatgtc gcagacccga accggccaat cctgctcgag caggcgcgcg
    18721 gccatggcgc cgcccatgtt gccgacgccc accacgccga tcgcgggttt catgcggcct
    18781 tcacgactgc accagccgct tggccttgga gatcgacatc aggatgccca ggcccatgcc
    18841 cagggtcgcc atcgccgtgc cgccatagct gatgaagggc aagggcacgc ccacgaccgg
    18901 cacgatgccg ctgaccatgc ccatgttgac gaaagcgtag gtgaagaaaa tcatggtcac
    18961 cgcgcccgcc agcaggcgcg agaacaccgt ggacgcctcc agcgcgatgg ccagcccgcg
    19021 cagcaccagg aacacgaagc cggcgatcag gaacaggttc ccgagcaggc cgaactcctc
    19081 cgagaacgcc gcgaagatga agtcggtggt gcgttccggg atgaattcca gatgagtctg
    19141 cgtgccctgc atgaagccct tgcccagcac gcccccggag ccgatggcga tcatgccctg
    19201 gataatgtgg aagcccttgc ccagcggatc ctttcggggg tccagcaggg tacagatgcg
    19261 ctgctgctgg taatcgtgga gcaccggcca gcgcacgccc tcggcgcaca cctgcggctc
    19321 gaacacgatc aggagaatag cggccgtgag gcccagcacc accggcggca agatcagttt
    19381 ccaggacagc cccgcgaaaa aaatcacgga caggcccgcc gcgagcacca gcaccgaggt
    19441 gcccaggtcg ggctgcttca cgatcaggcc cacgggaatg gccagcagca cgcccgcgac
    19501 gaggaagtcc agcgcccgca gctgcccctc gcgtttctgg aaccaccagg ccagcatcag
    19561 cggcgtg
//
LOCUS       DASWIY010000104        10004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3026546, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000104 DASWIY010000000
VERSION     DASWIY010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10004
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3026546"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VGA59_04070"
     CDS             complement(<1..681)
                     /locus_tag="VGA59_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9718885.1"
                     /translation="MKLKTAIALAALAIGAGQASAQNANQGVSKNEIVLGSIQDLSGP
                     IAGFGKQVRLGMLLRVEEINEQGGINGRKIKLIVEDSAYDPKKAVLAAQKLVNQDKIF
                     AMVGHIGTAQNMAAMPVQFEKNVINFFPVTAAREMYEPFHRLKYSFAATYYEQMRTAV
                     PKLVKDKGAKKVCTMYQDDEFGLEVMRGAEAGLKTIGMELAEKTSYKRGATDFSSQVA
                     RLKSSGCDM"
     gene            complement(1000..2856)
                     /locus_tag="VGA59_04075"
     CDS             complement(1000..2856)
                     /locus_tag="VGA59_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9718886.1"
                     /translation="MSNLWDLSHIQPETRVVLEGDTIPAMFWNGVEKRGSQIWMRQKK
                     LGIWRAMTWSQTGEAVREIAGGLMSLGFARGDTASILSNTVVEWVLADVAILSCGGVS
                     NGIYPTDAASQVHYLCEDSRTSVLFVEDDEQLDKALEVREQLPGLKKIVVFDMEGLRD
                     LNDPGVISLDHLRALGRDYLAAHPDELMQRVKSCKPQDLAILVYTSGTTGKPKGAMHS
                     QHGLVYTVRGYNTLVSQDEKDERMCFLPLCHIAERLGGEYFAMYTGAVLNFVENPETV
                     PENVREISPTVFTAVPRVWEKFYSGVMIGLKEASPLQQAVYSWGIGVGTQIAEKVMAG
                     RPVDGFLKFKFTLARWLALNNVRKLIGIHRSRFLVTGAAPISPELVKWYMALGVPMLE
                     VWGMTESCGAATGVPAMRIKPGSIGPAAAYNEVKLDPATSEILVRGDNVFMGYLNQPE
                     KTAETIDAEGWLHTGDVGLVDEDGFFRITDRMKDIIITAGGKNITPSELENELKFSPY
                     ITDAVVIGDKKAYLTVIIMIDQENVEKFAQDHDVPFSNYASLTKAPQVQELIQHEIDR
                     VNAKFARVEQIKKFFLLDTQLSAEDEELTPTMKLKRKLVQQKYAPQIEAMYR"
     gene            complement(2986..3783)
                     /locus_tag="VGA59_04080"
     CDS             complement(2986..3783)
                     /locus_tag="VGA59_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9718887.1"
                     /translation="MTHNPPAVSDTPVLKLLNVESAYGPIRAIRGVSLQVRRGEIATV
                     LGSNGAGKTTILKTISGIIDPRKGSIEFKGDSITAKDPAHIVQQGLSHVPEGREVFPL
                     LTVHDNLVMGAYTRHDRDGIARDMEAVYGYFPILKERSAQDAGLLSGGQQQMLAISRA
                     IMAAPDLILLDEPSLGLSPKLTREIFEIVVRINRERGTTILLVEQNANMALNASDYGY
                     VLENGRIVMEDTCARLREKEDIKEFYLGMKEAGVRGERRWKKKKNWR"
     gene            complement(3893..4789)
                     /locus_tag="VGA59_04085"
     CDS             complement(3893..4789)
                     /locus_tag="VGA59_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9718888.1"
                     /translation="MIDIETIRAAPGEAQQRTTSTLAQAQDLAGPGPADVLLSAKNLS
                     VRFGGVLAVNNVSFDIKPGEVFTLIGPNGAGKTTVFNLISRIYTPTAGSIEFEGKMLT
                     EQPPHKIASLGIARTFQNIELFEHATVLHNMLIGRHTHRNTGLWSEIFFTAKVRRAEI
                     DAREKVEKIIDFLDLQHYRDSMVAGLPYGVRKVVELARALCTEPKLLLLDEPSSGLNV
                     EETEDMAFWIQDIKNDLGITVLMVEHDMTLVSKVSDRVLAMNQGEVLAMGTPREVQNH
                     PGVIEAYLGSVDDMSSLRRGAV"
     gene            complement(4937..6013)
                     /locus_tag="VGA59_04090"
     CDS             complement(4937..6013)
                     /locus_tag="VGA59_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9718889.1"
                     /translation="MRFIFKTDYAQDIKLAKHGGHVFWYVLLLLLLLAAPWLFAEYWL
                     AQLTFVLIYSIVGLGLMLLAGFTGLFSIGHAAFLGVGAYTQAVFVNMGVPFPIALALA
                     GLLSAAVGVIVGLPALRVKGIYLGIATLAFGFIVEEVLARWESVTGGNSGIHVKHPDL
                     FGLKVDSATGFYFLCLGVAVVSTLGILNLLRSSTGRAFVAIRDSEISAQSMGIHLARY
                     KTLSFALSAALAGIGGALYAHKLTFISPDQFNILQSIDLLLMVVIGGLGSVHGAFLGA
                     IFLISMPQVISLTKDYLPPVIGQAPGLQGLVYGTVLIAFVLFEPLGLYGRWLKVRTYL
                     QLFPFYRKGLFKRQKSFQKSDRLR"
     gene            complement(6059..6955)
                     /locus_tag="VGA59_04095"
     CDS             complement(6059..6955)
                     /locus_tag="VGA59_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9718890.1"
                     /translation="MQFIQLVISGISQGCIYGLIALGFVLIYKATETVSFAQGELMML
                     GAFGGLASMTFLGFPFWLAVISAIAAMAVFGMLLERVVIRPILGQPAFSIVMLTIGIG
                     YVARGVITMIPAIGTETHTLPVPYKDMVWNVGGAAGGQGALVLNIEQMVVIGATGMLC
                     MLLFAVFRYSKVGIAMQASSQNQLAAYYMGIPVKRLNGLAWGLAAAVAAVAGLLLAPI
                     TFVHANMGFIGLKAFPAAVVGGFGSLPGAIVGGLIIGIVESLSGFYLPQGFKDVAAYV
                     VVLVMLMVKPSGLFGEKLRKKV"
     gene            7076..7903
                     /locus_tag="VGA59_04100"
     CDS             7076..7903
                     /locus_tag="VGA59_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9718891.1"
                     /translation="MEASVRVEKLGPVTLVTLNRPEVRNAVDAPTARALYEAFLGFDA
                     DAQARVAVFHGAHGHFCAGWDLQAGAQLARENSGAGVLGELDFTVDEPPSAGKGNTTA
                     PLAPMGPSRLLLSKPVIAAVSGAAVAGGMELALWCDMRVMEEDAYFGIYCRRFGVPLI
                     DGGTVRLPRLIGMGHAMDLILTGRKVEAAEALQMGLANRVVPRGAAREAALELARQLA
                     KFPQATMQSDRMNAYEQWDLPLSRALHLEWERSKHRIADALEGAARFAGGEGRHGKF"
     gene            complement(7985..8413)
                     /locus_tag="VGA59_04105"
     CDS             complement(7985..8413)
                     /locus_tag="VGA59_04105"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9718892.1"
                     /translation="MSANSSDPKGHTGLPGVALPLDKELVLKVIPMPADVNANGDVFG
                     GWVMAQVDLAGAVLPARYAKGRMATVAVKEFIFKQPVRVGDILSFYSEVTRIGRTSIT
                     VKVEVYAERFRTQGQYLKVTEASVTYVAIDEAGQPRPLPK"
     gene            8460..>10004
                     /locus_tag="VGA59_04110"
     CDS             8460..>10004
                     /locus_tag="VGA59_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX9718893.1"
                     /translation="MRGYGRHASAIPFEPGSTVAQPRAHTDWATLKRLFPYLWQYKWR
                     AAAALVFMVGAKLANVGVPVLLKNLVDTLNFKPGDPAALLVVPAGLLLAYGLLRLSTT
                     LFTELRELVFAKATQGAARSIALHTFQHLHALSLRFHLERQTGGMTRDIERGVRGIES
                     LISYSLYSIVPTIIEVTLVLSILAVKFDAWFAWITLTALALYIVFSVAVTEWRTKFRR
                     EANEFDSAAHTKAVDSLLNYETVKYFNNEGFEARRYDESLEKLRKARLKSQTTLSMLN
                     TGQQLIIAVGLVTMLWRATQGVVDGRMTLGDLVMVNAFMIQLYIPLNFLGVLYREIKQ
                     SLTDLDKMFVLMEKEREVADKPGAQPLASNDAMVRFENVGFAYEPARPILHHVSFEIP
                     AGKTVAVVGPSGSGKSTLARLMYRFYDVQQGRITIGGQDIREVTQASVRQAIGIVPQD
                     TVLFNDTVEYNIAYGRPGASREEVEEAARAARIHSFIESTPRGYQTMVGERGLKLSGG
                     EKQRVAI"
BASE COUNT         1728 a         3198 c         3240 g         1838 t
ORIGIN      
        1 catgtcgcaa cccgaggact tgagcctggc gacctgcgac gagaagtcgg tggcgccgcg
       61 cttgtagctc gtcttctcgg ccagctccat gccgatggtc ttcagtccgg cctcggcgcc
      121 gcgcatcacc tccaggccga attcgtcgtc ctggtacatg gtgcacacct tcttggcgcc
      181 cttgtccttg accagcttgg gcacggccgt gcgcatctgc tcgtagtagg tggcggcaaa
      241 ggagtacttc aggcgatgga agggctcgta catttcgcgc gccgcggtga ccgggaagaa
      301 gttgatcacg ttcttctcga actgcaccgg catggccgcc atgttctgcg ccgtgccgat
      361 gtggcccacc atggcgaaga tcttgtcctg gttcaccagc ttctgcgccg ccagcacggc
      421 cttcttcggg tcgtacgcgg agtcttcgac gatgagcttg atcttgcggc cgttgatgcc
      481 gccctgctcg ttgatttcct ccacccgcaa cagcatgccc aaccgcacct gcttgccgaa
      541 acccgcgatc ggcccggaca ggtcctggat ggagcccagc acgatctcgt tcttgctcac
      601 gccctggttg gcgttctggg cgctagcctg gccggcgccg attgccagcg cggcaagggc
      661 tatggcggtt ttcagcttca ttgttcgtct cctgttcaag gtaggggttg accgcctgca
      721 ttcttcccgg cctgcgggga gtgtcaagct ggcgtattca cttacgaggc aacccgaccg
      781 tggacaaaga aaacggccgg cggctttggg gtgtcatccc ggactcgatc ctcccgtttg
      841 tcatcccgga ttcgatcctc ccgtttgtca tcccggactc gatccgggat ccatgaagtc
      901 agcagtcatg gattgcgggt caagcccgca atgacaatct caacgggtca acccccaatg
      961 acaatctggg cgagtcaggc cctcaatggc cgcagggtcc tagcgataca tcgcctcgat
     1021 ctgcggcgcg tatttctgct gcaccagctt gcgcttcaat ttcatcgtcg gcgtgagctc
     1081 ttcgtcctcg gcggacagct gcgtgtccag caggaagaat ttcttgatct gctccacccg
     1141 cgcgaacttc gcgttgacgc ggtcgatctc gtgctggatc agctcctgca cctggggcgc
     1201 cttcgtgagg ctcgcatagt tgctgaaggg cacgtcgtgg tcctgggcga atttctccac
     1261 gttctcctgg tcgatcatga tgatcaccgt gaggtaggcc ttcttgtcgc cgatcactac
     1321 cgcgtcggtg atgtagggcg agaacttgag ttcgttttcc agctcgctgg gcgtgatgtt
     1381 cttgcccccc gccgtgatga tgatgtcctt catccggtcg gtgatccgga agaagccgtc
     1441 ctcgtccacc agcccgacgt cgccggtgtg aagccagccc tcggcatcga tggtttcggc
     1501 ggtcttttcc ggctggttca ggtagcccat gaacacgttg tcgccgcgca ccaggatttc
     1561 gctggtggcg gggtcgagct tgacctcgtt gtaggcggcg gccggcccga tcgagccggg
     1621 cttgatgcgc atggccggca cgcccgtggc ggcgccgcag gactcggtca tgccccacac
     1681 ttccagcata ggcacgccca gcgccatgta ccacttgacc agttccggcg agatcggcgc
     1741 cgcgccggtt accaggaagc gcgaacggtg gatgccgatc agcttgcgca cgttgttcag
     1801 cgcgagccag cgcgccagcg tgaacttgaa cttgaggaaa ccgtccaccg gccggcccgc
     1861 catcaccttc tctgcgatct gcgtgccgac gccgatcccc caggaataga cggcctgctg
     1921 cagcgggctg gcttccttca gcccgatcat cacgccggaa tagaactttt cccacacgcg
     1981 cggcacggcg gtgaacacgg tgggcgaaat ctcgcgcacg ttctcgggca ccgtttccgg
     2041 gttctccacg aaattgagga cggcgccggt gtacatcgcg aagtattcgc cgcccaggcg
     2101 ctcggcgatg tggcacagcg gcaggaagca catgcgctcg tctttttcgt cctgggacac
     2161 cagcgtgttg tagccgcgca ccgtgtagac caggccatgc tggctgtgca tcgcgccctt
     2221 gggcttgccg gtcgtgccgg aggtatagac caggatcgcc aggtcttgcg gcttgcagga
     2281 ctttacacgc tgcatcagtt cgtcgggatg ggcggccagg tagtcgcgac ccagcgcccg
     2341 cagatggtcc aggctgatca cgccgggatc attaaggtcg cgcaggcctt ccatgtcgaa
     2401 cacgacgatc ttcttcaggc ccggcagctg ctcgcgcacc tccaatgcct tgtccagctg
     2461 ttcgtcgtct tcgacgaaca ggacgctggt gcgcgagtct tcgcacaggt aatgcacctg
     2521 cgaggccgcg tcggtggggt agatgccgtt ggaaacgccg ccgcaactga ggatggccac
     2581 gtccgccagc acccattcga cgacggtgtt ggacaggatg gaagcggtgt cgccgcgcgc
     2641 gaagcccagg ctcatgagcc caccggcgat ctcgcgcacg gcctcgccgg tctgcgacca
     2701 tgtcatggcg cgccagatgc ccagcttttt ctgtcgcatc cagatctgcg agccgcgctt
     2761 ttccacgccg ttccagaaca tggcggggat ggtgtcgcct tcgagcacga cacgggtttc
     2821 gggctggatg tgggagagat cccaaagatt actcattcga agctccacgg ctgcaacgcg
     2881 ccggcgaaat caacacgggg acgccgcgga accggctttg ccgggccgcc ggcgtcgccc
     2941 cccagtgggg ggaggcggcc gcaggccgct tcggggggcg tcttttcatc tccagttctt
     3001 cttcttcttc cagcgccgct cgccgcgcac gccggcttcc ttcatgccca ggtagaactc
     3061 cttgatgtct tccttctcgc gcaggcgcgc gcaggtgtct tccatcacga tgcggccgtt
     3121 ctccaggaca tagccatagt cggaggcgtt gagcgccatg ttggcgttct gttccaccag
     3181 caggatcgtg gtgccgcgtt cgcggttgat ccgcaccacg atttcgaaga tctcgcgcgt
     3241 gagcttggga ctcaggccca ggctgggctc gtccagcagg atcaggtcgg gcgcggccat
     3301 gatggcgcgc gagatcgcca gcatctgctg ctggccgccc gagagcaggc cggcgtcctg
     3361 agcggagcgt tccttcagga tcggaaaata accgtacacc gcctccatgt cgcgggcgat
     3421 gccgtcgcgg tcgtggcggg tgtaggcgcc catcaccagg ttgtcgtgca ccgtcagcaa
     3481 cgggaacacc tcgcggcctt cgggcacatg cgacaggccc tgctgcacga tgtgcgccgg
     3541 gtccttggcg gtgatgctgt cgcccttgaa ctcgatgctg cccttgcgcg gatcgatgat
     3601 gcccgagatg gtcttgagga tggtggtctt gccggcgccg ttggagccga gcacggtggc
     3661 gatctcgccc cggcgcacct gcaggctgac gccgcggatg gcgcggatcg ggccgtacgc
     3721 ggactcgacg ttcagcagct tgagcacggg cgtgtcgctc acggcgggcg ggttgtgagt
     3781 catgactgca ttcctccctc tccctctggg agagggcagg ggtgagggcg ggcgcggctc
     3841 gatgccctca ccctaaccct ctcccggagg gagaggggac aagacagttc agtcataccg
     3901 cacccctcct gaggctgctc atgtcgtcga cagatcccag ataggcttcg atcacacccg
     3961 ggtggttctg cacttcacgc ggcgtgccca tggccagcac ctcgccctgg ttcatcgcca
     4021 gcacgcggtc ggacaccttg gacaccagcg tcatgtcgtg ttccaccatc agcaccgtga
     4081 tgcccaggtc gttcttgatg tcctggatcc agaacgccat gtcctcggtt tcttccacgt
     4141 tcaggcccga cgagggctca tcgagcagca gcagcttggg ctccgtgcac agggcgcgcg
     4201 ccagctcaac caccttgcgc acgccgtagg gcaggccggc caccatagag tcgcggtaat
     4261 gctgcaggtc gaggaaatcg atgatcttct ccaccttttc gcgcgcgtcg atctcggcgc
     4321 ggcggacctt tgcggtgaag aaaatctcgc tccacaggcc ggtgttgcga tgggtatgcc
     4381 gaccgatcag catgttgtgc agcaccgtgg cgtgttcgaa cagttcgatg ttctggaagg
     4441 ttcgggcgat gcccagcgag gcgatcttgt gcggtggctg ctcggtgagc atcttgcctt
     4501 cgaactcgat ggagccggca gtcggcgtgt agatgcggct gatcaggttg aagaccgtgg
     4561 tcttgccggc gccgttgggg ccgatcaggg tgaaaacctc gcccggcttg atgtcgaagc
     4621 tgacgttgtt gacggccagc acaccgccga agcgcacgct caagttcttg gccgagagca
     4681 ggacatcggc tggccccggc cctgccaggt cctgagcctg cgcaagggtg gaggttgttc
     4741 gctgctgggc ctcgcctggc gccgcgcgaa tggtttcaat atcaatcatg tcgttttcca
     4801 tcgcgctgcc gcatcaccgg cgccgcatcc atcggatcag gccattcgtg ggccaccgcg
     4861 gatccggctt tgccgggccg ctggtggcgc ccccttgagg gggaggcggc cgaaggccgc
     4921 ttcgggggtg ggcttatcat ctgagtcggt ccgatttctg gaaggacttc tgccgtttga
     4981 acaggccctt gcggtagaag gggaagagtt gcaggtaggt gcgcactttc agccagcgac
     5041 cgtacaggcc cagcggctcg aacagcacga acgcgatcag caccgtgccg tagaccagcc
     5101 cctgcaggcc cggggcctgc ccgatcacgg gcggcaggta gtccttggtc agggagatca
     5161 cctgcggcat ggagatcagg aagatcgctc ccaggaaggc gccgtgcacg gatccgaggc
     5221 cgccgatcac caccatcagc agcaggtcga tcgactgcag gatattgaac tggtcgggcg
     5281 agatgaaggt cagcttgtgc gcgtacagcg cgccgccgat gccggccagc gcggccgaca
     5341 gcgcgaacga cagcgtcttg tagcgcgcca ggtggatgcc catgctttgc gcggagattt
     5401 ccgagtcgcg gatggcgacg aatgcgcgcc cggtggacga gcgcagcagg ttcaggatgc
     5461 ccagcgtgga cacgaccgcg acacccaagc aaaggaaata aaaaccggtg gcggagtcga
     5521 ctttcagtcc gaacaggtcg gggtgcttga cgtggatgcc cgagttgccg ccggtgaccg
     5581 attcccagcg cgccagcact tcctcgacga tgaagccgaa ggccagcgtc gcgatgccca
     5641 ggtagatgcc tttcacccgc agcgccggca agccgacgat gacgccgacg gccgccgaaa
     5701 gcaggcccgc cagcgccagc gcgatgggga acggcacccc catgttgacg aacacggcct
     5761 gggtgtaggc gccgacgccg agaaaggccg cgtgcccgat cgagaacagg ccggtgaagc
     5821 cggccagcag catcaggccc aggcccacga tcgagtagat cagcacgaag gtcagctgcg
     5881 ccagccaata ctcggcgaac agccacggcg cggcgagcag cagcagcaac agcagcacgt
     5941 accagaagac atggccgccg tgcttggcca gcttgatgtc ctgggcgtaa tcggttttga
     6001 agatgaagcg cattgttagt cggttgggga cagagctgaa gatctgtccc gcaaggtctc
     6061 atactttctt tcttagcttc tcgccgaaca ggccgctggg tttcaccatc agcatgacca
     6121 gcacgacgac ataggcggcg acgtccttga agccctgcgg caggtagaag cccgagagcg
     6181 attcgacgat gccgatgatc agcccgccga cgatggcgcc cggcaggctg ccgaagccgc
     6241 cgaccaccgc cgcggggaag gccttcagcc cgatgaatcc catgttggcg tgaacgaacg
     6301 tgatgggcgc gagcagcagg ccggccaccg cggccacggc cgcggccagg ccccaggcca
     6361 ggccgttgag ccgtttcacg gggatgccca tgtagtaagc ggccagctgg ttctgcgacg
     6421 aggcctgcat cgcgatgccg accttgctgt acctgaacac ggcgaacagc agcatgcaca
     6481 acatgccggt tgcgccgatc accaccatct gctcgatgtt cagcaccaga gcgccttgtc
     6541 cgccggcggc gccgccgaca ttccacacca tgtccttgta ggggacggga agcgtgtggg
     6601 tttcggtgcc gatcgccggg atcatggtga tgacgccgcg tgccacatag ccgatgccga
     6661 tggtcagcat cacgatcgag aaggcgggct gtcccaggat cggccgtatc acgacacgct
     6721 cgagcaacat gccgaacacg gccatggcgg cgatggcgct gatcacggcc agccagaacg
     6781 ggaagccgag gaaggtcatc gacgccaggc cgccgaacgc ccccagcatc atgagctcgc
     6841 cctgggcgaa actgactgtc tcggtggctt tgtagatgag cacgaagccc agcgcgatca
     6901 ggccatagat gcatccctgc gagatgccgc tgatcaccag ttgaatgaat tgcaccttct
     6961 ctccatgact tgaggcccgg ttataacttt caggggtctg catcatgtag agggttggcc
     7021 cgaatccgtg tcgcgaagtg gaccgggagc acccgtttca ggtgtaatcc cctaaatgga
     7081 agcaagcgtc cgcgtcgaga aactggggcc cgtcacgctg gtgacgctca accgtccgga
     7141 agtgcgcaat gccgtggacg cgcccacggc gcgcgccttg tacgaggctt ttctcggctt
     7201 cgatgcagac gcgcaggcgc gagtcgccgt gttccacggt gcgcacggcc atttctgcgc
     7261 cgggtgggac ttgcaggccg gggcgcaact ggcgcgtgaa aacagcggtg ccggcgtgct
     7321 gggcgaactc gacttcacag tggatgagcc accgtcggcc gggaagggca acacaacagc
     7381 accgctggct cccatgggcc cctcgcggct gctgctgtcc aagcccgtca tcgccgcggt
     7441 gagcggcgcg gcggtcgcgg gcggcatgga gctggccctc tggtgcgaca tgcgcgtgat
     7501 ggaggaggac gcctatttcg gcatttactg ccgccgcttc ggcgtgccgc tgatcgacgg
     7561 cggcacggtg cggctgcccc ggctgatcgg catgggacac gcgatggacc tcatcctcac
     7621 cggccgcaag gtcgaggcgg ccgaggcgct gcagatgggc ttggccaatc gcgtcgtgcc
     7681 caggggcgcg gcgcgcgagg cggcgcttga actggcgcgg cagctggcga agttcccgca
     7741 ggccaccatg cagtcggacc ggatgaatgc ctatgagcaa tgggacctgc ccttgtcccg
     7801 ggcgctgcac ctggagtggg agcgcagcaa gcaccgcatc gccgatgccc tggaaggcgc
     7861 ggctcgcttc gccggcggcg aagggcggca cgggaagttc tagcgccgcc tgctgcccac
     7921 gggcagagag cgtggatccc ggatccagtc cgggatgaca tctatttgga gtccgggatg
     7981 acgtctattt cggcagcggc cgcggctggc cggcctcgtc gatcgccacg taggtgaccg
     8041 acgcttccgt caccttgagg tattgcccct gcgtgcggaa gcgctcggca tagacctcca
     8101 ccttcaccgt gatcgaggtg cggccgatgc gcgtgacctc ggaatagaac gagaggatgt
     8161 cgcccacccg caccggctgc ttgaagatga attccttcac ggccacggtc gccatgcggc
     8221 ctttggcgta gcgcgccggg agcaccgcgc cggccaggtc cacctgggcc atcacccagc
     8281 cgccgaagac gtcgccgttg gcgttgacgt ccgccggcat ggggatcacc ttgaggacca
     8341 attccttgtc caggggcagt gccaccccgg gcagaccggt gtgacccttc gggtcgcttg
     8401 aattagcgga catgggcaca atctctctct tcagtaacac caactcccga ttgtccccga
     8461 tgcgcggtta cggccgtcac gcttctgcca tcccattcga acccggctcg accgtggcgc
     8521 agccgcgcgc gcacaccgac tgggccacgc tcaagcggct gtttccctac ctgtggcagt
     8581 acaagtggcg ggcagcggcg gcgctggtct tcatggtggg cgccaagctg gccaacgtcg
     8641 gcgtgccggt gctgctgaag aacctggtcg acacgttgaa cttcaagccg ggcgatccgg
     8701 cggccctgct ggtggtgccc gccggactgc tgctggctta cggcctgctg cggctttcga
     8761 ccacgctgtt caccgaattg cgcgagctgg tgttcgccaa agcaacgcag ggggcggccc
     8821 gcagcatcgc cctgcatact ttccagcacc tgcatgcctt gagcctgcgc ttccacctgg
     8881 agcgacagac cggcggcatg acgcgagaca tcgaacgcgg cgtgcgcggc atcgaatccc
     8941 tgatctcgta ttcgctctac agcatcgtgc ccaccattat cgaggtgacg ctggtgctgt
     9001 cgatcctggc ggtgaagttc gacgcctggt ttgcctggat cacgctcacg gcgctggcgc
     9061 tgtacatcgt tttctccgtt gccgtgaccg aatggcgtac caagttccgc cgcgaagcca
     9121 acgagttcga ttcggctgcc cacaccaagg cggtcgactc gctgctgaac tacgaaaccg
     9181 tcaagtactt caacaacgag ggcttcgagg cccgccgtta tgacgagagc ctggagaagc
     9241 tgcgcaaggc ccgcctgaag agccagacca cgctgtcgat gctcaacacc gggcagcaat
     9301 tgatcatcgc cgtgggcctg gtcacgatgc tgtggcgcgc aacgcagggc gtggtcgatg
     9361 gccgcatgac gctgggcgac ctggtgatgg tcaacgcgtt catgatccag ctgtacatcc
     9421 cgctgaattt cctgggcgtg ctgtaccgcg agatcaagca gagcctcacc gacctggaca
     9481 agatgttcgt gctgatggaa aaagagcgcg aggtggcgga caagcccggc gcgcagccgc
     9541 tggcaagcaa cgacgccatg gtccgcttcg aaaacgtcgg cttcgcctac gagccggcac
     9601 gccccatcct gcaccacgtg agcttcgaga tcccggccgg caagaccgtg gccgtggtcg
     9661 gcccatccgg ttcgggcaag tcgacgctgg cgcgcctgat gtaccgcttt tacgacgtgc
     9721 agcaaggccg aatcacgatc ggcggccagg acatccgtga agtcacgcag gcgagcgtgc
     9781 ggcaggccat cggcatcgtg ccgcaggaca cagtgctttt caacgacacg gtggaataca
     9841 acatcgccta tgggcgcccc ggcgcgagcc gcgaggaagt ggaagaagcc gcgcgcgccg
     9901 cccgcatcca cagcttcatc gaatccacgc ccaggggata ccagacgatg gtgggcgagc
     9961 gcggcctgaa gctttccggc ggcgagaagc agcgcgtggc gatc
//
LOCUS       DASWIY010000105        10035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3027755, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000105 DASWIY010000000
VERSION     DASWIY010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10035
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3027755"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..421)
                     /locus_tag="VGA59_04115"
     CDS             complement(188..421)
                     /locus_tag="VGA59_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9718894.1"
                     /translation="MDIKVLGTGCTNCKSTIALIEQIAKAKGVSVTLNKVEELRDIMG
                     YGVMSTPGVVIDGKVVHAGGVPSREKIEQWLAA"
     gene            complement(452..1504)
                     /locus_tag="VGA59_04120"
     CDS             complement(452..1504)
                     /locus_tag="VGA59_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX9718895.1"
                     /translation="MTELTLSQRTLQLPRQHPRVFLALAALAWWALYQALIPASEALV
                     AALPVDRDTHLGGALQFFFYDTPKVLLLLTAVVFVMGMVNSYFTPERTRALLAGKTEG
                     IANVMAASLGIVTPFCSCSAVPLFIGFVQAGVPLGVTFSFLISTPMVNEVALALLFGM
                     FGWKIALLYMGLGLTVAIVAGWVIGRLKMEPYLEDWVRDMPRIQAAAGEARMTLGERI
                     DAGMASVREIVGKVWPYILVGIAIGGAIHGWVPQDFMASFMGKDAWWSVPLAVAIGVP
                     MYSNAAGVIPIVQALLVKGAALGTVLAFMMSVIALSLPEMIILRKVLKVRLIATFVGV
                     VATGILIVGFVFNTVL"
     gene            complement(1581..2156)
                     /locus_tag="VGA59_04125"
     CDS             complement(1581..2156)
                     /locus_tag="VGA59_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9718896.1"
                     /translation="MNSDLPPPTARFACVAAAWQAHEGELRGYLRHRLSDTDAADDVL
                     QDVFVKAMRQGQGFCALDSPRAWLFQVARNALVDRLRTTHRHEPLTDELANFIPLDTQ
                     PVAPVDALADCLSRTLGELPAEDAAILRACDLEGQTQRAFAEAKGLSLPAVKSRLLRA
                     RQRLRERMSQACQVRFEADGSVASHVPRPPP"
     gene            complement(2162..3931)
                     /gene="arsA"
                     /locus_tag="VGA59_04130"
     CDS             complement(2162..3931)
                     /gene="arsA"
                     /locus_tag="VGA59_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HEX9718897.1"
                     /translation="MKFLDQPPRFLFFTGKGGVGKTSIACASAIRLAEAGQRVLLVST
                     DPASNVGQVFGIAIGNRVTAIPGVARLSALEIDPQAAAQAHRDRVVGPVRGVLPDTVV
                     KSIEEQLSGACTTEIAAFDEFTELLTNQALLADYEHVVFDTAPTGHTIRLLQLPGAWS
                     GFLDAGKGDASCLGPLAGLEKQRTQYKDAVDALADPGRTRLVLVARAQQATLREVART
                     HDELAAIGLSQQYLVINGVLPRSEASQDPLAAAIFEREQAALAAIPDVLRPLPTDHVP
                     LKPFNLVGLDALQQLLDALERPAAELTDEPTELAAPTLASLIDEIALDGHGLVMLMGK
                     GGVGKTTLAAAVAVALADRGLPVHLTTSDPAAHLSETLMGARVNLTVSRIEPQVETER
                     YRQHVLETKGAQLDDQGRALLEEDLRSPCTEEIAVFQAFSRIIREAGRKFVVMDTAPT
                     GHTLLLLDATGAYDREIQRQMGGKGIHFTTPMMQLQDSKQTKVLLVTLAETTPVLEAA
                     NLQADLRRAGIEPWAWIVNQSVAAAQPRSPLLRQRAYNERAEIDAVAGMHARRHAVVP
                     LLLHEPVGVDRLLELSDRVAAVA"
     gene            complement(3957..4385)
                     /gene="arsD"
                     /locus_tag="VGA59_04135"
     CDS             complement(3957..4385)
                     /gene="arsD"
                     /locus_tag="VGA59_04135"
                     /inference="COORDINATES: protein motif:HMM:NF018637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HEX9718898.1"
                     /translation="MKTIQVFDPALCCSTGVCGVEVDQALVSFSADVDWAQGQGAKIE
                     RFNLAQQPMAFAEHAAVKGALERSGQDALPLIVMDGKVALSGRYPTRDELAQWLGLSQ
                     GTNETSAGAKLAAIAVASVPGKACCGPAAGTADKSASKCC"
     gene            complement(4399..5490)
                     /gene="arsB"
                     /locus_tag="VGA59_04140"
     CDS             complement(4399..5490)
                     /gene="arsB"
                     /locus_tag="VGA59_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX9718899.1"
                     /translation="MSTSTLHAPTAAPAPLNVFERYLTLWVFLCIVAGIVLGQLLPGP
                     IQAIGRMEFAQVNIPVGVLIWVMIIPMLLKVDFGALSQVKGHWRGIGVTLFINWAVKP
                     FSMALLGWIFIRQVFAPYLPAEQLDSYIAGLILLAAAPCTAMVFVWSRLTKGDPMFTL
                     SQVALNDSIMVFAFAPIVALLLGLSSITVPWDTLLTSVGLYIVIPVILSQWWRKSLLR
                     RGQAAFDAAMHKIGPWSVAALLATLVLLFGFQGKAILDQPLIIAMLAVPILIQVFFNS
                     GLAYWLNRRVGESHRVACPSALIGASNFFELAVAAAISLFGFQSGAALATVVGVLIEV
                     PVMLLVVRIVNASKPWYERGAAVRARVQR"
     gene            complement(5502..6008)
                     /locus_tag="VGA59_04145"
     CDS             complement(5502..6008)
                     /locus_tag="VGA59_04145"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX9718900.1"
                     /translation="MTVHVLILCTHNSARSVLAEGMLNHWAAKLGKDVRAHSAGSAPS
                     GRINPLAIEALSAAGIATDGYRSKSWDEFSAEGAPPLSIVITVCDSAAAETCPVFFGG
                     SGGQPVKVHWSYPDPSNAEGGDEGKRRAFELTRQAIGYRMLQLLALPLETRDRAVLQT
                     ALAEIGRN"
     gene            complement(6022..6366)
                     /locus_tag="VGA59_04150"
     CDS             complement(6022..6366)
                     /locus_tag="VGA59_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9718901.1"
                     /translation="MDEYRAVVSLAALAQGMRLRIFRTLIGAGPDGMTPGALALMLGV
                     NSSTLSFHLKELLHSGLVSQERDGRNLIYRPALPHMTELVDYLTAHCCDATGGQCDLP
                     KKKTRRAASSAV"
     gene            complement(6495..7013)
                     /gene="mobB"
                     /locus_tag="VGA59_04155"
     CDS             complement(6495..7013)
                     /gene="mobB"
                     /locus_tag="VGA59_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX9718902.1"
                     /translation="MKIFGIAGHSGMGKTTLLERLVPEIASLGLVVSLIKHSHKNIDI
                     DRPGKDSYRLRESGCKEVLLLGNDRWALMHELRGAVEPSLDYLLDRMQHCDLVLIEGF
                     KHGEFPKVEVWRAELEKPTLWPQWPGIVAIASDGPAAVPPQAVDGPAITRLDLSDISA
                     IAAFVLSCAVAR"
     gene            complement(7098..7724)
                     /locus_tag="VGA59_04160"
     CDS             complement(7098..7724)
                     /locus_tag="VGA59_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEX9718903.1"
                     /translation="MSIPSTDGSIFRNGGSPGFLRRFWARLNRPSRFSFLAVSTIFFV
                     GGVLFWGGFHTGVDMTNTLEFCTTCHEMRDTVYQEYKQSVHYSNRAGIRAICSDCHVP
                     REWSHMMVRKMQASLELWGKVTGTIDTRDKFEARRMQLALHEWERLQESNSRECRNCH
                     AFDAMSPGLQKPEPYKIHMQAREEGKTCIDCHKGLAHKLPKGYNILNQ"
     gene            complement(8001..8648)
                     /locus_tag="VGA59_04165"
     CDS             complement(8001..8648)
                     /locus_tag="VGA59_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9718904.1"
                     /translation="MKIEVLVADDHAIIRDGLKKILADTGDLAVAGEAANGNAALEKV
                     RERDWGLVVLDMSMPGRSGLELIKMMKAERPRLPILVFSMHQEEQFAVRAIRAGASGY
                     LSKEGDSDMLLPAMRRVASGGAFVSDKVAQLLATDVSKGGDCPAHTLLSDREYGIFSR
                     IVRGASLTEIADELCLSIKTVSTHKSHILAKMNLAGQVDLVRYAIEHNLLDPARE"
     gene            complement(8645..>10035)
                     /locus_tag="VGA59_04170"
     CDS             complement(8645..>10035)
                     /locus_tag="VGA59_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX9718905.1"
                     /translation="VPHRENLIGEYAPELGNRTLPAPGDEVRMVDHATGDGGRQAFAL
                     GRTGRFPLLVGVPNDVELVLAAWRENSVVIVLGAILVCAFVVTAAGLLVRGVNRQAEL
                     ARALRDANDRYHQTIDSVMDAIIAVDASHNIMLFNPAAERMFGRAKQDVIGQPLAQLV
                     PERVREAHILHMDRFMCTKGESRAMATNVEITGLRSDGTEFPIESTISQTLIEGKPQL
                     TAVLRDITRRRRNEENLQEMNRQLRSLSASLQDVREQERTRIAMELHDDLGQQLTGLK
                     LELSWLGGRLKEGRQATPDEVGAMRRMLDAAIASVRRIATELRPLILDDLGFGEAVAW
                     QIAEFAKRSGLDITLDLQAQALIKDDVLATALFRIVQESLTNVVRHAGATKVQVRLVR
                     DEDDLVLTVNDNGSGMAAGPRTGSGIGLVSMRERATSLGGQLNIAGVPGTGTTIQVTL
                     PLGRTAQEKETA"
BASE COUNT         1799 a         3310 c         3201 g         1725 t
ORIGIN      
        1 acgtgatcac gaaagtcatc aggaacacca tcatcgcgcc catcaggagc gagaagatca
       61 ggtgaaagcg ctgtgcggga agtttactca tgacaaagac atggggatga gacgatcagg
      121 ccgagaatct gacatggcca agcggcccgt tgtcatcagc aggcgacctg ggtctcatcg
      181 cacgctccta ggcggccagc cattgttcga tcttctcgcg actgggcacg ccacccgcgt
      241 gcaccacctt gccgtcgatc acgacgcccg gcgtgctcat gacgccgtag cccatgatat
      301 cgcgcagctc ctcaaccttg ttcagcgtga cactgacgcc cttggccttg gcgatctgtt
      361 cgatcagcgc gatcgtgctc ttgcagttgg tgcaaccagt gccgagtacc ttgatgtcca
      421 ttaaaagctc cttggagggt tgagaagatg atcacagcac cgtgttgaaa acgaagccca
      481 cgatcagaat gccggtggcg accacgccaa cgaaggtggc gatcaaacgc accttgagga
      541 ccttgcgcag gatgatcatc tcgggcagcg acagcgcgat cacgctcatc atgaaagcca
      601 gcacggtgcc cagcgccgcg cccttgacca gcagcgcctg cacgatcggg atcacacccg
      661 cagcattgct gtacatcggg acaccgatcg ccacggccag cggcaccgac caccaggcgt
      721 ccttgcccat gaacgaggcc atgaagtcct gcggcaccca gccgtggatg gccccgccga
      781 tggcaatgcc gaccaggatg tagggccaga ccttgccgac gatctcgcgc acactggcca
      841 tcccggcatc tatgcgctcg ccaagtgtca tccgcgcttc accggccgcg gcctggatcc
      901 tgggcatgtc gcgcacccag tcttccagat agggttccat cttcaggcgg ccgatgaccc
      961 atcctgcgac gatcgcgacc gtaaggccca ggcccatgta aagcaaggcg atcttccagc
     1021 cgaacatgcc gaacagcagc gccagggcga cttcgttgac cataggtgtc gatatgagga
     1081 atgagaaggt cacgcccagc ggaacgcccg cctggacgaa tccgatgaac agcggcacgg
     1141 ctgagcacga gcaaaacggt gtcacgatgc ccaggctggc tgccatgaca ttggcgatgc
     1201 cttcagtctt gccggccagc agcgcccggg tgcgctcggg tgtgaagtag gaattcacca
     1261 tgcccatcac gaacaccacg gcagtgagta acagcagcac cttgggcgtg tcgtagaaga
     1321 agaactgcag tgcaccgccg aggtgggtgt cgcggtccac cggcagggcc gccaccagcg
     1381 cctcggacgc cggaatcagc gcttggtaca gcgcccacca agccagcgcc gcgagcgcca
     1441 ggaagacccg tggatgctgc cgaggcagct gcagggtccg ttgcgatagg gtcagttcgg
     1501 tcatccgggt gtctccactt gagaagacga acagacccgc aaaaggatgc acacctgccc
     1561 tgggaaatgg atcccgatcc ttacggcggt ggccgcggca catgagaggc gacgctgcca
     1621 tcggcctcga aacgcacctg acacgcttgc gacatccgct cgcgcagccg ctgccgcgcc
     1681 cgcagcaggc gcgacttgac ggccggcagg ctcaaccctt tggcctcggc aaaggcgcgc
     1741 tgggtctgcc cctccaggtc gcaggcgcgc aggatcgcgg cgtcctcggc cggcagctcg
     1801 ccgagcgtgc gcgacaggca gtcggccaga gcgtcgacgg gggcaaccgg ctgcgtgtcc
     1861 agcggaatga agttggccag ttcgtcggtc aagggctcgt gccggtgcgt cgtgcgcaac
     1921 cggtccacca gcgcattgcg cgccacctgg aacagccagg cgcgcgggct gtccagcgcg
     1981 caaaagccct ggccctggcg catcgccttc acaaacacat cctgcagcac atcgtcggcg
     2041 gcatcggtgt ccgacaaccg gtgccgcaga tagccgcgca actcgccctc atgagcctgc
     2101 caggcggcgg ccacacaggc gaacctggcg gtcggaggtg gcaggtcgct gttcatgccg
     2161 ttcaggccac agccgccacg cggtccgaca gttccagcag gcggtccacg cccaccggtt
     2221 catgaagcaa tagcggaacc actgcatggc ggcgcgcatg catgccagcg acggcatcga
     2281 tctcggcgcg ctcgttgtac gcacgctggc gcagcagcgg agagcgcggc tgggccgccg
     2341 cgacgctttg gttgacgatc caggcccagg gctcgatgcc cgcgcgccgc aggtcggcct
     2401 gcaggttggc cgcttcgagc accggtgtgg tctccgccag cgtgaccagc agcaccttgg
     2461 tctgcttcga gtcctgcagt tgcatcatcg gcgtcgtgaa gtggatgccc ttgccgccca
     2521 tctggcgctg gatctcccgg tcgtaggcac cagtcgcgtc cagcagcaat agggtgtgcc
     2581 ccgtgggcgc cgtatccatc acgacgaact tgcggcccgc ctcgcggatg atgcgcgaga
     2641 aggcctggaa cacggcaatc tcctcggtgc agggggaacg caggtcttct tccagcagcg
     2701 ccctgccctg gtcgtcgagc tgagctcctt tggtttccag cacatgctgg cggtagcgct
     2761 cggtctcgac ctgcggttcg atgcggctca cggtgagatt cacacgtgcg cccatcagcg
     2821 tttcgctcag gtgcgccgct gggtcggagg tggtcaggtg gaccggcaag ccgcggtcgg
     2881 ccagcgccac ggcgaccgcg gctgccaacg tggtcttgcc gacgccgcct ttgcccatca
     2941 gcatcaccag gccatgaccg tccagcgcga tctcgtcgat cagcgacgcg agggtgggtg
     3001 cggccagctc ggtcggctcg tccgtaagct cggcggcggg gcgctccaat gcatcgagca
     3061 actgttgcag cgcatcgagc cccaccaagt tgaacggttt gagcggcacg tggtctgtcg
     3121 gcaagggccg cagcacgtcc gggatcgccg ccaacgcggc ttgctcgcgc tcgaaaatcg
     3181 ctgcggccag cgggtcctgc gaggcctcgc tgcgcggcag aacgccattg atgacgaggt
     3241 attgctgcga gaggccaatc gcggcgagct cgtcatgcgt gcgcgccact tcgcgcaacg
     3301 tcgcctgctg ggcgcgcgcc accagcacca ggcgcgtgcg ccctgggtcg gccagagcgt
     3361 ccaccgcgtc cttgtactgc gtgcgctgct tttccaggcc ggccagcggg ccaaggcagg
     3421 aggcgtcgcc cttgccggcg tcgaggaagc cgctccaagc ccccggcagt tgcagcaggc
     3481 ggatggtgtg gccggtgggt gccgtgtcga agacgacgtg ctcatagtcg gccagcaggg
     3541 cctggttggt gagcagttcg gtgaactcgt cgaacgccgc gatctcggtc gtgcaggcgc
     3601 ccgagagctg ttcctcaatg ctcttgacga cggtgtcagg cagtacgccg cgcaccgggc
     3661 cgacgacgcg gtcgcggtgg gcttgcgcgg cggcctgcgg gtcgatttcc agggccgaca
     3721 ggcgcgccac tccgggaatg gcggtgactc gattgccgat ggcgatgccg aagacctgcc
     3781 ccacgttaga cgccgggtcg gtgctgacca gcaggacccg ctggccggct tcggctagcc
     3841 ggatggcgct ggcgcaggca atcgaggtct tgcccacgcc gcccttgccg gtgaagaaca
     3901 ggaaacgtgg tggctggtcg aggaatttca tggggcactc cgccggcgcc cgccggtcag
     3961 cagcacttgg atgccgactt gtcagctgta ccggcggccg ggccgcaaca agccttgccc
     4021 ggaacgcttg ccacggcgat ggcggccaac ttggcgccgg cacttgtctc gttggttccc
     4081 tgcgacagcc cgagccattg cgccagctca tcgcgcgtgg gatagcggcc cgacagagcg
     4141 acctttccgt ccattacgat caacggcagc gcgtcctggc cgcttcgctc cagcgcgcct
     4201 ttgaccgccg catgctcggc gaaagccatc ggctgctgcg ccagattgaa ccgctcgatt
     4261 ttcgcgcctt ggccttgggc ccagtccacg tccgccgaga agctgaccag cgcctggtcc
     4321 acttcgactc cacacacacc agtgctgcag cacagcgccg ggtcgaacac ctggatcgtt
     4381 ttcatgtaca aactccaatc atcgttgaac tcttgctctc accgctgcac cgcgctcgta
     4441 ccagggcttg ctggcattga cgatacgcac caccagcagc atcaccggca cctcgatcag
     4501 cacgcccacc acggtggcca gcgccgcgcc cgactggaag ccgaagagac tgatcgctgc
     4561 cgccaccgcc agctcgaaga agttgctggc gccgatcagc gccgacgggc aggccactct
     4621 gtgcgattcg ccgacgcggc ggttgagcca gtaggccaag cccgagttga agaagacctg
     4681 gatcaggatc ggtacggcaa gcatcgcaat gatcagcggc tgatcgagga tcgccttgcc
     4741 ctggaaacca aacagcagca ccagcgtggc gagcaacgcc gccaccgacc acggaccgat
     4801 cttgtgcatc gcggcgtcga aggcggcctg cccacgcctg agcagcgact tgcgccacca
     4861 ctgagacaga atcaccggaa tcacgatgta caggcccacc gatgtgagca aggtgtccca
     4921 cggaaccgtg atcgacgaca gccccaacag gagcgcgacg atgggtgcga acgcgaacac
     4981 catgatgctg tcgttcagcg ccacctgcga cagcgtgaac atcgggtcgc ctttggtcag
     5041 ccggctccaa acgaacacca tcgccgtgca cggcgcggca gccagcagga tcaggcccgc
     5101 gatgtagctg tcgagctgct cagccggcag gtagggcgca aagacctggc ggataaagat
     5161 ccagcccagc agcgccatcg aaaacggctt gaccgcccag ttgatgaaca gcgtcacgcc
     5221 gatgccccgc cagtggccct tgacctggga gagcgcgccg aagtccacct tcagcaacat
     5281 tgggatgatc atcacccaga tcagcacgcc caccggaatg ttgacctgcg cgaattccat
     5341 gcggccgatc gcctggatgg gccccggcag caactggccc agcacgatgc cggcgacgat
     5401 gcacagaaag acccacagcg tcaggtagcg ctcgaagacg ttcaacggcg ctggcgcggc
     5461 ggtgggggcg tgcagtgtgc ttgtgctcat cgtggcgttc ctcagttgcg gccgatctcg
     5521 gccagtgcgg tttgcagaac ggcgcggtcc cgggtttcca acggcagggc cagcagttgc
     5581 agcatgcggt agccgatcgc ctgacgcgtg agctcgaacg cgcggcgttt gccttcgtcg
     5641 cctccttcag cattggaagg atcgggatag ctccaatgga ccttgaccgg ctggccgccg
     5701 ctgccgccaa agaagaccgg acaggtttcg gcagccgcgc tgtcgcacac ggtgatcaca
     5761 atcgacagcg gcggagcacc ttcggcgctg aattcatccc agctcttgct gcggtagccg
     5821 tcggtggcga tgccggcggc gctcagcgcc tcgatggcca agggattgat ccggccgctg
     5881 ggcgcgctgc ccgcgctgtg ggcgcgaacg tccttgccga gcttggccgc ccaatggttg
     5941 agcatccctt cggccagcac gctgcgagcc gagttgtgcg tgcagagaat gagaacgtgg
     6001 actgtcatgg gcttggaaaa attaaacagc actcgaagcg gcacgcctgg tcttcttctt
     6061 gggcaaatcg cactgaccgc cggtcgcatc acagcaatga gcagtcaggt aatccaccag
     6121 ctccgtcatg tgcggcagcg ctggccggta gatgaggttt cgcccatcac gctcctgcga
     6181 aacaagcccc gaatgcagca gttccttcag atggaatgag agcgtcgaag agttcacgcc
     6241 caacatgagc gccaaagctc cgggggtcat tccgtccggc cctgccccga tcaatgtgcg
     6301 gaagatgcgc aaccgcatgc cttgcgccag ggcagccagg gaaacaacag cgcgatattc
     6361 atccatattt ccaatcctat caaactatta ggccaagcag tgcgtccgga ttgccgcgac
     6421 ggtgaagtgg gctggatgcc cagggacgcc gaaagtgaga attccaggat tggggtaacc
     6481 acacggggca cacatcatcg cgccaccgcg catgacagga cgaacgcagc aatcgccgag
     6541 atatccgaca aatcgagtcg ggttatcgct ggtccgtcga cggcttgcgg cggcacggcc
     6601 gccggcccat cgctggcgat tgccacgatg cccggccact ggggccacaa cgtcggcttc
     6661 tccaattcag cccgccagac ttcgaccttt ggaaactcac catgcttgaa gccctcgatc
     6721 agaaccaggt cgcaatgctg catgcgatcc agcagatagt ccagcgatgg ctccacggcg
     6781 ccgcgcaatt catgcatcag cgcccaccgg tcattgccaa gaagcagtac ttccttgcag
     6841 ccggattcgc gcagccgata tgagtccttg cccggccgat cgatatcgat gttcttgtgg
     6901 ctgtgcttga tgagcgacac caccaggccg agcgaagcga tttcggggac cagccgctcc
     6961 agaagcgttg tcttgcccat gccggaatgg ccggcgatgc cgaatatttt catgacagga
     7021 gaatgactgc gaatacgcta ttttgcccgc tgcctgcaat ggagtccgcc ggaaattttt
     7081 cgaaggctcg gcgcgagtca ctggttcaag atgttgtagc ccttgggcag cttgtgcgcg
     7141 aggcccttgt ggcaatcgat gcatgttttt ccctcttccc gggcctgcat gtgtatcttg
     7201 tagggctcag gcttctgcaa tcctgggctc atcgcatcga acgcgtggca attgcggcac
     7261 tcgcgcgagt tgctctcctg cagccgctcc cattcgtgca gggccaactg cattcgcctg
     7321 gcctcgaatt tgtccctcgt gtcgatggtg ccggtgacct tgccccagag ttcgaggctc
     7381 gcctgcatct tgcgcaccat catgtgcgac cattccctgg ggacatggca gtccgagcag
     7441 atcgcgcgaa tgccggcacg gttgctgtag tggacactct gcttgtattc ctggtagacc
     7501 gtgtcgcgca tctcgtggca ggtcgtgcag aattccagcg tgttcgtcat gtccacgccg
     7561 gtgtggaacc caccccagaa gaggacgccg ccgacaaaga aaatggtcga tacggccagg
     7621 aacgagaacc tgctcggccg gttcagacgc gcccagaacc gccgcagaaa tccgggactg
     7681 ccgccgttcc tgaaaattga cccatcggtc gaaggtatgg acatgtttca gggcagccgc
     7741 gatccgaacg gctgcgtccg gcaatgcggc ggccggcgac acttcacgcc gcaatccctg
     7801 tcgcgactga ctggttcatc tgtccccctc gcgaggcgcc ggcacggacc gggccatgat
     7861 cgggtgatat tgatcggaac ggcagcgcct gaatatcggc cggcgctcaa agtggccatc
     7921 ggacatttcc gatggcgggc ggggagcgga cccgcggatg cccgcgcgat ccgcagcccg
     7981 caggcagagg aggctgcggc ctactcgcgg gccggatcga gcaggttgtg ctcgatggcg
     8041 tagcgcacca ggtcgacctg gccggccagg ttcatcttcg ccaggatgtg ggacttgtgg
     8101 gtgctcacgg tcttgatgct caggcaaagt tcgtcggcga tctcggtcag gctggcgccg
     8161 cgcacgatgc ggctgaagat gccgtactcg cggtcgctca ggagcgtgtg ggccggacag
     8221 tccccgcctt tggagacgtc ggtggccagc aactgcgcga ccttgtcgct gacgaaggcg
     8281 cctcccgagg ccaccctgcg catcgccggc agcagcatgt cgctgtcgcc ttccttggac
     8341 agatagccgg atgccccggc gcgtatcgcc cggacggcga actgctcttc ctggtgcatg
     8401 ctgaaaacga ggatgggcag cctgggccgc tccgccttca tcatcttgat cagctccagc
     8461 ccgctgcgcc cgggcatcga catgtccagg acgaccagcc cccagtcgcg ctcgcggacc
     8521 ttctccagcg ccgcattgcc attggcggcc tcgccggcca cggccaagtc gccggtgtcc
     8581 gccaggattt ttttcaggcc atcccggatg atggcgtggt cgtccgcgac cagtacttcg
     8641 atcttcatgc ggtttccttt tcctgtgccg tccttccgag cggcaaggtc acttggatgg
     8701 tggtgccggt gcccgggacg ccggcgatgt tgagctgccc gccgagcgag gtggcgcgct
     8761 cgcgcatgct gaccaggcca atgccgctgc ccgtcctggg gccggcggcc atgcccgatc
     8821 cattgtcgtt cacggtcagc accaggtcgt cttcgtcgcg gacaaggcgt acttgcacct
     8881 tggtggctcc ggcatggcgc acgacattgg tcaacgactc ctgcacgata cggaacaacg
     8941 cggtggccag cacgtcgtcc ttgatcagcg cctgcgcttg caggtcgagc gtgatgtcca
     9001 gcccgctgcg cttggcgaat tccgcgatct gccaggccac tgcttcgccg aaccccaggt
     9061 cgtcgaggat cagcggccgc agctcggtgg cgatgcggcg caccgacgcg atcgccgcgt
     9121 cgagcatgcg gcgcatggcg cccacctcgt ccggcgtggc ttgccggccc tctttcaggc
     9181 gtccgcccag ccaggaaagc tccagcttga gcccggtcag ctgctgcccc aggtcgtcgt
     9241 gcagttccat cgcgatgcgg gtccgctcct gttcgcgcac gtcctgcagc gaggccgaca
     9301 ggctgcgcag ctgccggttc atctcctgca ggttttcctc gttccggcgg cgccgggtga
     9361 tgtcgcgcag aacggccgtc aactggggct tgccctcgat cagggtctgc gagatcgtcg
     9421 actcgatcgg gaactcggtg ccgtccgagc gcagcccggt gatttcgacg ttcgttgcca
     9481 tggcgcggga ttcgcctttg gtgcacatga accggtccat gtgcaggatg tgagcctcgc
     9541 gcacgcgctc agggaccagc tgggccaggg gctggccgat cacgtcctgc ttggcccggc
     9601 cgaacatgcg ctcggccgcc gggttgaaca gcatgatgtt gtgggacgcg tccaccgcga
     9661 tgatggcatc catgaccgaa tcgatcgtct gatggtagcg atcgttggcg tcgcgcagcg
     9721 cccgcgcgag ctccgcctgg cggttgacgc cgcgcaccag caacccggcg gcggtgacca
     9781 cgaaggcgca gaccaggatc gcgcccagga cgatgacgac ggagttctcg cgccaggcgg
     9841 ccagcaccag ttccacgtca ttgggaaccc ccaccagcaa aggaaagcgc cccgtccgcc
     9901 cgagggcgaa agcctgccgg ccaccatcgc cggtggcatg gtcgaccatg cggacttcgt
     9961 cgccgggggc gggcagcgtc cggttgccga gctccggcgc atattcgccg atcaggttct
    10021 cccggtgcgg cacgc
//
LOCUS       DASWIY010000106         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2291996, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000106 DASWIY010000000
VERSION     DASWIY010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291996"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VGA59_04175"
     CDS             <1..1432
                     /locus_tag="VGA59_04175"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEX9718906.1"
                     /translation="RIPHHGLGLSVDVYQPDLFELADELHGRGLDCGYLEIFKAAPAA
                     LEAVRRRLPDVLLAYHAEGLWVTQPHPERLSPFHAELDGTAEQLRILGSHWLNHECAA
                     KQMAGYAFGTYLPALFTAASAEVTADNIAQAQQRLDDGHQGTGPLFLLETPPLTYVGF
                     GDLSMAEYFRLVTDRTPCGVVLDIGHLWTVYRYTGEWRRRSLTSFLTELLDAFPLERV
                     VEVHMAGLAPHPCVPSEAGQAGEPPLWIDAHGAPIPEVLSDMLAQVLEHDGLTHLKGV
                     ALEVDTKPIGMIVEEFARAHARFGPKIAHALRDERDERDLRDSNPAIPAFPASLALPA
                     SLASLAEQYDCYAQIVTGRATDGLPVPVLGQDASALPLYVEHYLPYEILEWGGVVRDM
                     FPETCRNLDRAGIPLDGFVQFWFREPRESSESGQAYDFFLLKITRFIEFIREELPAAS
                     GTVEREAESLRSGYEAACAQVGSETR"
     gene            1429..2640
                     /locus_tag="VGA59_04180"
     CDS             1429..2640
                     /locus_tag="VGA59_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX9718907.1"
                     /translation="MTPHAGFWAQLPHPIIGLAPMDGVTDAPFRFMVATQGRPDVIFT
                     EFTSVMDVCRGPAHMLSSLIYSEAERPVVAQLYGKDPAVFYQAAHIVCELGFDGLDIN
                     MGCPSRNVASSGSGAGLIRTPDLAHEIMRATRQGIADWAGGQSLAGAGLKDSRVDMIR
                     AMTRRHGGPPAARRVIPLSVKTRIGYDSIIVERWIEHLLGEQPVAISVHGRTLAQMYR
                     GEADWSAIERAAQLARQTGTLLLGNGDVRSLQDLVLRVRQHGVHGVLVGRGALGAPWL
                     FRHKETARAAVSDPARTVERESPALEERFLVMLDHARQYEALLDRQCFPRMRKHLGWY
                     CKGFPSAAAMRAAMVRASSSEDVARLVSDYQAEQKARHQIQPHVEPRAQQPDDSAHRT
                     MPALAAAAPVA"
     gene            2802..>3210
                     /locus_tag="VGA59_04185"
     CDS             2802..>3210
                     /locus_tag="VGA59_04185"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX9718908.1"
                     /translation="MPPLILASTSPRRKELLALLQVPFEMVEPNYHEWTLAEVPPEAQ
                     ARTFALEKARSCMWRHPDSLVVGSDTLIALGTMNLGKPADRAQANAMLRRLRGREHRI
                     CSAVAVCGGARQIEDVALDIVQVWMKPFSDADLE"
BASE COUNT          521 a         1080 c         1064 g          545 t
ORIGIN      
        1 ccgcatcccc caccacgggc tgggtctgtc cgtggacgtc taccagcccg acctgttcga
       61 gttggcggat gaactacacg ggcgcgggct ggactgcggc tatctcgaaa ttttcaaggc
      121 ggctccggcc gcgctggaag cggtccgccg gcgcctgccc gacgtcctgc tcgcgtacca
      181 cgccgaaggg ctctgggtca cgcagccgca tcccgagagg ctctcgccgt ttcacgccga
      241 gctggacggc acggccgagc agctcaggat cctcggcagc cattggttga accatgaatg
      301 cgcggccaag cagatggccg gctatgcctt cggcacctat ctgccggcat tattcacggc
      361 ggccagcgcc gaggtcacgg cggacaacat cgcgcaggcc cagcagcgtc tggatgacgg
      421 ccaccagggg acaggcccgc tgtttttatt ggaaacgccg ccgctcacct atgtgggctt
      481 cggcgacctg tccatggcgg agtatttccg cctggtgacc gaccgcacgc cctgcggggt
      541 ggtgctggat atcggccacc tctggaccgt ctatcgctac accggcgaat ggcggagacg
      601 ttcgttgact tccttcctga ccgagctgct ggacgccttt ccgctggagc gcgtcgtcga
      661 ggtccacatg gccgggctgg cgccgcatcc gtgcgtacca tcagaggccg gtcaggccgg
      721 agagccaccg ctgtggatcg acgcgcatgg cgcgccgatt ccggaggtgc tctccgacat
      781 gttggcgcag gtcctcgagc atgatggatt gacccatctg aagggcgtgg cgctggaagt
      841 cgacacgaag ccgatcggca tgatcgtgga ggagttcgcg cgggcccatg cgcgattcgg
      901 tcccaaaatc gcgcatgcgc tgcgcgacga gcgggacgag cgcgacttgc gcgactcgaa
      961 ccccgccatt ccagcctttc ctgcgagtct cgcgcttccc gctagtctcg cgagtctcgc
     1021 cgagcaatat gattgctacg cgcaaattgt tacggggcgg gcgacggacg gcctgcctgt
     1081 gcccgttctt ggccaggatg cctcggcgct gccgctctat gttgagcact acctgcccta
     1141 cgagatcctg gaatggggcg gcgtggtgcg ggacatgttc ccggaaacct gccgaaatct
     1201 cgatcgagca gggatcccgc tggatggctt cgttcaattc tggttccgcg agccgcgcga
     1261 gtcgagtgaa tccggccagg cctatgactt tttcctgctg aagatcaccc gcttcatcga
     1321 gttcattcgc gaagaattac cggccgcatc cggcacggtc gagcgggaag ccgaatcgtt
     1381 gcgcagcggg tatgaagcgg cctgcgcgca ggtcgggagc gaaacgcgat gacgcctcac
     1441 gccggcttct gggcccaatt gcctcatccg atcatcggcc tcgcgcccat ggacggcgtc
     1501 accgatgcgc cgttccgctt catggtggcc acgcagggcc ggccggacgt catcttcacc
     1561 gagttcacca gcgtgatgga cgtctgccgg ggccccgcac acatgttgtc gtcgctcatc
     1621 tacagcgaag cggagcgtcc ggtcgtggcc cagctctacg gcaaggatcc ggccgtcttc
     1681 taccaggcag cgcacatcgt ctgcgagctg ggattcgacg ggctggacat caacatgggc
     1741 tgtccctcgc gcaacgtcgc ctcctccggc tccggcgccg ggctcatccg cacgcccgac
     1801 ctggcgcatg agatcatgcg ggcgacgcgg cagggcatcg ccgattgggc gggcgggcaa
     1861 tcgctcgccg gcgcgggact gaaggactcg cgcgtcgaca tgatccgcgc catgacccgg
     1921 cggcacggcg gcccgccggc cgcgcgccgg gtcatcccgc tctcggtcaa gacgcgcatc
     1981 ggctacgact cgatcatcgt cgagcgctgg atcgaacatc tgctggggga gcagccggtc
     2041 gccatttcgg tgcacgggcg cacgctggcg cagatgtacc ggggcgaggc cgactggtcg
     2101 gccatcgagc gggccgcgca attggcgcgg cagaccggta cgctcctgct cggcaacggc
     2161 gacgtgcgga gtctgcaaga tctcgtcctg cgggtccggc agcacggcgt gcacggggtg
     2221 ctggtcggcc gtggcgccct gggggcgccc tggctgttcc gccacaagga gacggcccgc
     2281 gccgcggtga gcgatcccgc gcgcacggtc gaacgggagt cgccggcgct cgaagaacga
     2341 ttcctcgtca tgctggacca cgcgcggcag tacgaggcgc tgctggaccg gcagtgtttt
     2401 ccccgcatgc gcaagcacct gggctggtac tgcaaggggt tccccagcgc cgccgcgatg
     2461 cgggccgcca tggtgcgggc ctccagcagc gaggacgtcg cgcgcctcgt atccgactac
     2521 caggccgagc agaaggcccg gcatcagatc cagcctcacg ttgagcctcg tgcgcaacag
     2581 ccggatgatt ccgcgcaccg caccatgccc gcgcttgcag ccgcagcgcc cgtcgcctag
     2641 cccacattac tcatgaacgc ttctactgca accaccgaca cgccccacgg cagagtgttg
     2701 agcctgttat gcccgttaat aacggtgaaa cgggccgaac gggctcaacg gctcaacaag
     2761 tgggcggttt cgtcacgaac cgccatgaat aatgtgggct aatgcccccg cttatcctcg
     2821 cctccacctc gccgcgccgc aaagagctcc tggccctctt gcaggttccc ttcgagatgg
     2881 tcgaaccgaa ttaccacgaa tggactcttg ccgaggtgcc gccggaggcc caggcgcgga
     2941 ccttcgcgct ggagaaggcc cgctcgtgca tgtggcgcca tcccgacagc ctggtagtgg
     3001 gaagcgacac gctgatcgcc ttggggacga tgaatctcgg caagccggcc gatcgcgcgc
     3061 aggccaacgc catgttgcgg cggctccgtg gccgcgagca ccgcatctgc agcgcggtcg
     3121 cggtctgtgg cggggcgcgc cagatcgagg acgtggccct ggacatcgtg caagtctgga
     3181 tgaagccgtt cagcgacgcc gacctggagg
//
LOCUS       DASWIY010000107         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3029213, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000107 DASWIY010000000
VERSION     DASWIY010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3029213"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VGA59_04190"
     CDS             complement(<1..841)
                     /locus_tag="VGA59_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HEX9718909.1"
                     /translation="MLDLLITNATLPDGRTGMSVAVQGGRITEVTPGLRAPAQQTVDA
                     AGHLLTSPFCDPHFHMDSALTWGNPRVNESGTLLEGIAIWGELKPTLKAEDIIQRALT
                     YCDWAVARGLLAIRSHVDTSDPSLLPVEALLEVKRRVAPYIDLQLVAFPQDGVLRTPG
                     GVDNLKRALDLGVDIVGGIPHFERTMAEGAASVKLLCEIAAGRGKLVDMHCDETDDPM
                     SRHIETLAFEAQRLGLQGRVTGSHCTSMHSMDNYYVSKLVPLIAESGVSIVANPLINI
                     TLQG"
     gene            complement(1127..>1714)
                     /locus_tag="VGA59_04195"
     CDS             complement(1127..>1714)
                     /locus_tag="VGA59_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX9718910.1"
                     /translation="LDGTWHIWIGYDHILFLLALLLPSVLIHRRKRWEAVSRPGPAIR
                     EVVKVVTAFTLAHSITLSLAALQVISLPSRLVESAIAASVVLAALNNLRGTIEKRRWV
                     VAFVFGLIHGFGFASVLTDLGLPQQALVLALVGFNVGVEIGQLAIVAVFLPLAFGLRR
                     TGFYRVGVLKAGSTGVALLAGWWFVQRAFDIQSPF"
BASE COUNT          298 a          549 c          600 g          267 t
ORIGIN      
        1 tgccctgcag cgtgatgttg atcagcgggt tggcgacgat gctcacgccg ctctcggcga
       61 tcagcgggac gagcttgctc acgtagtagt tgtccatgct gtgcatggag gtgcagtgcg
      121 agcccgtcac tctgccctgc aggcccaagc gctgggcctc gaaggccagg gtctcgatgt
      181 ggcgcgacat gggatcgtcg gtttcgtcgc agtgcatgtc caccagcttg ccgcgcccgg
      241 ccgcgatctc gcacagcagc ttcacgctgg cggcgccttc cgccatggtg cgctcgaagt
      301 gcgggatgcc gccgacgatg tccacgccca ggtccagcgc gcgcttgagg ttgtccacgc
      361 cgcccggggt gcgcagcacg ccgtcttgcg ggaaggcgac cagttgcagg tcgatataag
      421 gcgcgacccg gcgcttgact tccagcagcg cctccacggg aagcaggctg gggtcgctgg
      481 tgtccacatg gctgcggatc gccaacaggc ccctggccac cgcccaatcg caataggtga
      541 gggcgcgttg gatgatgtcc tcggccttga gggtgggttt gagctcgccc cagatcgcga
      601 tgccctccag cagcgtgccg ctttcgttga cgcgtggatt gccccacgtc agtgcggagt
      661 ccatatggaa atgcggatcg cagaagggcg acgtgaggag gtgcccggcg gcgtcgacgg
      721 tttgctgcgc gggggcgcgg aggccggggg tcacctcggt gatgcgaccg ccttgtacgg
      781 cgaccgacat gccggtgcgg ccgtcgggaa gggtggcgtt ggtgatgagg aggtcgagca
      841 tgggcagatt ctctgtcatg ccgggctggg ctcgcggaac gcccggggcg cgcagctgtc
      901 cagtgcagcg agcccaatgc cgggcctacg ctatgcacgt gccgaggcat cgcgctccgg
      961 cccggcccgg catccacgtc ggctgggcgc agcgatggat tgcgggtcaa gcccgcaata
     1021 acagccgctt gggcgccgcg atggattgcg ggtcaggccc gcaatgacaa ggccagggtc
     1081 aagccgcaat gacaaaacca ggttcaagcc cgcgatgaca gcctttctag aacgggctct
     1141 ggatgtcgaa ggcccgctgg acgaaccacc agccggccag gagcgcgaca cctgtcgagc
     1201 cggccttgag tacgccgacc cggtagaacc cggtgcggcg caagccgaac gccagcggca
     1261 ggaacacggc gacgatcgcc agctggccga tttccacgcc gacgttgaag ccgaccagtg
     1321 ccagcaccag ggcctgctga ggcaagccga ggtccgtgag cacggacgcg aagccgaagc
     1381 catggatcag cccgaagacg aaggccacga cccaccggcg cttttcgatg gtgccgcgca
     1441 ggttgttcag agccgccagc acgaccgagg cggcgatcgc cgactccacc agccgcgagg
     1501 gcaggctgat tacctgcaag gcggccaggc tcagcgtgat cgaatgcgcc agcgtgaacg
     1561 cggtcaccac tttgacgact tccctgatcg ccggtccggg gcgcgatacg gcttcccacc
     1621 gctttcgccg atggatcagc acggacggca gcagcagcgc cagcaggaac aggatatggt
     1681 cgtagccgat ccagatatgc caggtgccgt ccag
//
LOCUS       DASWIY010000108        11541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1547650, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000108 DASWIY010000000
VERSION     DASWIY010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547650"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="VGA59_04200"
     CDS             complement(<1..1895)
                     /locus_tag="VGA59_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9718911.1"
                     /translation="MSAGLNLAQQEAVNYMHGPCLVLAGAGSGKTRVITHKLGRLIQA
                     GLQANRIAAITFTNKAAAEMRERAKSLIGRDAKEVLICTFHALGVRMLRRDGAALGLK
                     PTFSILDSDDVTGILKDAGGTTDAATARQWQWAISLWKNLGLNAAQAEAQAKDDNEKV
                     TAHIMARYEERLAAYQSVDFDDLIGLPLKLLQQHPEVREKWQAQMGHVLVDEYQDTNA
                     TQYEVLKLLVGERGRFTAVGDDDQSIYGWRGATLDNLKRLPLDFPNLKVVKLEQNYRS
                     TGAILRAANNVIGPNPKLFPKTLFSELGEGEPVRIVDADNEAHEAERVVARIQSTRAN
                     GQSQEWKDFAVLYRANHQAKVFEQALRKVQIPYKVSGGQSFFDRAEIKDLCAWLRLVV
                     NNDDDPAFLRAVTSPKRGIGHQTLQALGVFAGKYRLSLFEALFSSSLGSVLAGRAVAG
                     LHEFGRYVNDLEFRARRTTGAEDARVFLTDWLKDIGYEKHLYDSEENEKLAASRWTNV
                     VEFCDWMAQRCGGQAGDAAGVSIASEKKTLLEVAQTISLLSTISEREQDQNVVTLSTL
                     HAAKGLEWPHVMLVGVTEGLLPFKLGDDDNPTGRADGPMSEGILQRLQEERRLMYVGI
                     TRAQRSLA"
     gene            2002..2967
                     /locus_tag="VGA59_04205"
     CDS             2002..2967
                     /locus_tag="VGA59_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX9718912.1"
                     /translation="MLDILLVTFPFFVLVLFGYIAARRRMLPFDAISGLNTFVLYFAL
                     PCMLYRFGAGTPIAQLLDAVAFSVYLLCALTLVALVVWASHNSRIGWNDASFGALVAA
                     FPNTGFMGVPLLVALLGAAAAGPAILTIMIDLVITSSLCIALSRLDGADEHGVEKAAK
                     SALRGVAMNPMPWAIVLGGVASALQLEPVKPLSQTIGLLADSASPVALFTIGAVLARS
                     QFVAAQEEHDPMPLRDFVPVAVTKLVVHPLLVWGAGSLAIALGLPLDRFALAVMVLVA
                     ALPSASNVALLAERFGADTGRIARIILLTTAAAFLTFSAAVALVK"
     gene            3145..4062
                     /locus_tag="VGA59_04210"
     CDS             3145..4062
                     /locus_tag="VGA59_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX9718913.1"
                     /translation="MARPPEPSSLTIEFLLGIDGGGSGTRVVLARRDGPVLGRGEAGP
                     SALGQGIAQAWRNVERACRAAFDAARLPVPPWDRCAAGAGLSGISNRPWRDAFVAANI
                     GFARLEAETDSFVMLLGAHRGKPGAILAVGTGSVAEALRPDGSRHTVGGWGFPAGDEG
                     SGAWLGLHAVRHAQCALDGRVNAGPLARRVWALCGDERDTLQDWCDGAGQFAYAQLAP
                     AVFECEGTDPAAASLLLEATAALETMALAVDPKGRLPLAVCGSIGLRLAPRLAPAVRS
                     RLVPAAQGPAAGALMLIRQAAHAEMEELV"
     gene            4059..5174
                     /gene="nagA"
                     /locus_tag="VGA59_04215"
     CDS             4059..5174
                     /gene="nagA"
                     /locus_tag="VGA59_04215"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX9718914.1"
                     /translation="MSLTLSGHILGPQGFVAGTMELDGSGRVRRITGAPVAASALRDS
                     GQPLVLPGFIDLHVHGGGGRDIMEGSGAASRVAQCHAAHGTTALLATTMTAPMADLET
                     AFTGLRSSCDERPARGARVLGVHLEGPYINPGKLGAQPDFARPVSMEELARLDAIAPI
                     RLITLAPEMPGNMEQVKALSEAGYHVQLGHTLGSYEDGVQALALGARGFTHLFNAMTP
                     LHHRAPGMVGAALAHARYAEIIPDLLHVHAGALRVALRSIPCLYCVTDSTAATGMPDG
                     DYRLGRQTVTKCLGGVRLADGTLAGSTLTMDQALRNLVDELGLSLAEASMRLSTHAAD
                     YLGLADRGRLAAGAWADVVVLDRDLQLTDVFVEGVST"
     gene            5183..6193
                     /locus_tag="VGA59_04220"
     CDS             5183..6193
                     /locus_tag="VGA59_04220"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX9718915.1"
                     /translation="MLVEACEAPAAVAQQLAQGSSAYRDLGALLRSQPPTSLLTVARG
                     SSDYAANYVGYLIMARMGRLVTSLPMSLVTLYQSKIACRGLASFAFSQSGQSPDLVEP
                     TRYFREGGAMTSAFVNDPASPLARAAQWVFPLHAGVESSVAATKSVIAQMCAGARLVA
                     AWQDDGALQTALSALPQVLSRAAGADWSAALDVLKDADRLLVIGRGLGLPVALEAALK
                     FKETCAVQAEAFSGAEVQHGPMALIEEGYPLLVFAPRGPAQPGLLALADAMRSRGARV
                     LLAAPAGTPRTSLPIAETASAELDPISMLQSFYPMVEALSRSRGLDPDQPRHLSKVTR
                     TQ"
     gene            6282..6992
                     /locus_tag="VGA59_04225"
     CDS             6282..6992
                     /locus_tag="VGA59_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HEX9718916.1"
                     /translation="MKALLVTCMLAAAPFVWASDKTAPRAPAATAVTGEVLEIQNVES
                     YTYLRLKTAQGETWAAVTTAPVKKGAKVTIENATVMNNFESKALKKTFPSVVFGTLAG
                     AQGVAGAAAGAAGPHSGAGKPKETAADVHVPRATGANARTVAEIMTKPAALKDKAVLV
                     RGKVVKYNPGIMGKNWLHLRDGSGTAAAGTNDVLVTTTEQAKVGDIVTAKGVLRTDRD
                     FGAGYSYKAIVEEATLQR"
     gene            complement(7039..7257)
                     /locus_tag="VGA59_04230"
     CDS             complement(7039..7257)
                     /locus_tag="VGA59_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718917.1"
                     /translation="MSTDRDSYKSLQAEYTAAVAQERAEWKVASDASLGAVERVLAYA
                     RWLAAAERVKALSIRLGDHAPPAWPCRP"
     gene            complement(7455..8498)
                     /gene="murQ"
                     /locus_tag="VGA59_04235"
     CDS             complement(7455..8498)
                     /gene="murQ"
                     /locus_tag="VGA59_04235"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX9718918.1"
                     /translation="MSDEKRRSPGQDRHRSNVHLEERAAMKLELSQPAAKPARKPMTP
                     AKPLDARLTEQRNPRSTRIDQLSTIGIVDLINAEDRMVAAAVGEERQQIARAIDLVVD
                     CLRKGGRLVYVGAGTSGRLGVLDASEMPPTYRTEPEMVQGIIAGGTDALLRAQEGAED
                     HPEDGAAAIDEKNIGENDFVLGIAASGTTPYVHGALKRARERGARTGFLLCTYPSERL
                     IASHDVVIAPLVGPEVITGSTRMKAGTATKIILNTISTAAMVKTGKVYGNLMVDLQVT
                     CQKLQDRGERILMETLGVKREEASRLLDASGGHVKTAIVISKLSLGAEEARRRLEEVG
                     GSIADLVGNLRIG"
     gene            complement(8662..10494)
                     /locus_tag="VGA59_04240"
                     /pseudo
     CDS             complement(8662..10494)
                     /locus_tag="VGA59_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
     gene            10563..>11541
                     /locus_tag="VGA59_04245"
     CDS             10563..>11541
                     /locus_tag="VGA59_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX9718919.1"
                     /translation="MPSLVPLVELLRGGTLECVHFGAVAVSDAQGKLVASAGDPHWVS
                     FSRSTLKPLQALPFVQGGGPGQLGFSREQVAMMCASHSGEPMHVAQVEAMLDKAGLSY
                     RALQCGCHMPFFAELGAWPPPAPGSYDERHHNCSGKHSGFLACCVQHGWPVANYLAPA
                     HPLQQAIRRDVARAVGLAAEDVKMGIDGCSAPNYAMPLVNLARGYARLAGGAADGEFG
                     ASFAQLADAMTAHPELVSGTGRNDLAFMRAGRGDWISKGGAEGMHALASRSRRQALVV
                     KVADGNKIAVQAATVAVLDQLGWLDARQREELRPWRAEAIANARGTQVGE"
BASE COUNT         1644 a         3966 c         4001 g         1930 t
ORIGIN      
        1 gccagcgagc gctgggcccg ggtgatgccc acgtacatca ggcggcgctc ttcctgcagg
       61 cgctgcagga tgccttcgga catcggccca tcggcgcgcc ctgtggggtt gtcgtcgtcg
      121 ccgagcttga acggcagcag cccttcggtc acgcccacca gcatgacgtg cggccattcc
      181 aggcccttgg ccgcatgcag ggtggacagc gtgaccacgt tctggtcctg ttcgcgctcg
      241 ctgatcgtgg acagcagcga gatggtctgc gccacctcga gcaatgtctt tttctcgctg
      301 gcgatgctga caccggccgc atcgcccgcc tggcccccgc agcgctgcgc catccagtcg
      361 cagaactcca ccacgttggt ccagcgcgag gcggccagtt tctcgttttc ctcgctgtcg
      421 tacaggtgct tttcgtagcc gatgtccttg agccagtcgg taaggaagac gcgcgcatcc
      481 tccgcgccgg tggtgcggcg ggcgcggaac tccaggtcgt tcacatagcg gccgaactcg
      541 tgcagtcccg ccacggcgcg gccggccagc acggagccga gcgaggagga gaacagggcc
      601 tcgaacaggc tgagccggta cttgccggcg aacacgccca gcgcctgcag ggtctggtgg
      661 ccgatgccgc gcttgggcga ggtcacggcg cgcagaaagg ctggatcgtc gtcgttgttg
      721 acgaccaggc gcagccaggc gcacaggtcc ttgatctcgg cgcgatcgaa aaagctctgg
      781 ccgcccgaaa ccttgtacgg gatctggacc ttgcgcaagg cctgctcgaa taccttggcc
      841 tggtggttgg cgcggtagag caccgcgaaa tccttccact cctggctctg gccgttggcg
      901 cgcgtcgact ggatgcgcgc cacgacgcgc tcggcttcgt gcgcctcgtt gtcggcgtcg
      961 acgatgcgta ccggctcgcc ctcgcccagt tcggagaaca gggtcttggg gaacagcttg
     1021 gggttgggcc cgatcacgtt gttggccgcg cgcagaatcg caccggtgga gcggtagttc
     1081 tgctccagct tcacgacctt caggttcggg aagtccagcg gcaggcgctt caggttgtcc
     1141 agcgtggccc cgcgccagcc gtagatcgac tggtcgtcgt cgcccacggc ggtgaaacgc
     1201 ccgcgctcgc ccaccaggag cttgagcacc tcgtattgcg tcgcgttggt gtcctggtat
     1261 tcgtccacga gcacatggcc catctgcgct tgccatttct cgcgcacctc ggggtgctgc
     1321 tgcagcagtt tgaggggcag gccgatcagg tcgtcgaagt ccacgctctg gtaggccgcg
     1381 agccgctcct catagcgcgc catgatgtgc gccgtcacct tttcgttgtc gtctttcgct
     1441 tgggcctcgg cctgcgccgc attgagtccc aggttcttcc agaggctgat cgcccactgc
     1501 cactggcgcg cggtggccgc gtcggtggtg ccgcccgcgt ccttcaggat gccggtcacg
     1561 tcatcgctgt cgaggatcga gaacgtgggt ttgagcccca gtgccgcgcc gtcccggcgc
     1621 aacatgcgca cccccagggc atggaaggtg cagatcagga cttcctttgc gtcgcggccg
     1681 atcaggctct tggcccgctc gcgcatttcg gccgcggcct tgttggtgaa ggtgatggcg
     1741 gcgatgcggt tggcttgaag gccggcctgg atcagccgcc ccagcttgtg cgtgatcacc
     1801 cgcgtcttgc cggagccggc gccggccagc accaggcagg gcccatgcat gtaattgact
     1861 gcttcttgct gggcgaggtt caggccggcg gacatctggg aaagtggggg agggactgcg
     1921 gaggaagcgg cgaatcatac cggccggcgc cgtcaggaag atgaaaatcg cggcccgggc
     1981 agttgcgcgg agaatacgcc cgtgcttgac atcctgctcg tcacctttcc cttcttcgtg
     2041 ctggtgctgt ttggctatat cgcggcgcgg cgccgcatgc tgccctttga cgcgatttca
     2101 ggactcaaca ccttcgtgct ctatttcgcg ctgccctgca tgttgtaccg cttcggcgcc
     2161 ggcacgccga ttgcgcagct gctggatgcg gtggcgttct cggtgtacct gctgtgtgcg
     2221 ttgacgctgg tcgcgctggt cgtctgggcc agccacaact cgcgcatcgg ctggaacgac
     2281 gcatcgttcg gcgcgctggt ggcggcgttt cccaacaccg gtttcatggg cgtgccgctg
     2341 ctggtggccc tgctcggcgc ggcggctgcc ggcccggcca tcctcaccat catgatcgac
     2401 ctggtgatta ccagttcgtt gtgcattgcg ctgtcgcgcc tggacggcgc cgacgaacac
     2461 ggcgtcgaaa aggccgcgaa gagcgcgcta cgcggcgtgg ccatgaaccc catgccctgg
     2521 gccatcgtgc tgggcggcgt ggcctcggcg ctgcaactgg agccggtgaa gccgctctcg
     2581 caaacgatcg gcctgctggc cgattcggct tcgcccgtgg ccttgttcac gatcggcgcg
     2641 gtgctggcgc gctcgcagtt cgtcgccgcg caggaagaac acgacccgat gccgctgcgc
     2701 gacttcgtgc ccgtcgcagt caccaagctg gtcgtgcacc cgcttttggt atggggcgcg
     2761 ggcagcctgg ccatcgcgct gggcctgccg ctggaccggt tcgcgcttgc ggtgatggtg
     2821 ctggtcgcgg cgctgccttc ggccagcaac gtggccctcc tcgccgagcg gttcggcgcc
     2881 gacaccggac ggatcgcgcg catcatcctg ctgaccacgg ccgcggcctt cctgaccttc
     2941 tcggcggcgg tggcgctggt caaatgatgc actcgcccaa tcagcggaac aggcccggtc
     3001 tctggccagc gccagcccgc tgtcggcgta agccgcgcga gggcgtggga cttgtcccac
     3061 ggtgctgcca aaaacgcgct gcaactcccc gccgggaccc ggctccaaca tggatgaaca
     3121 gctttcgcca tggatgggcc tggtatggcg cgcccgcctg aaccgtcctc actgaccatc
     3181 gaattcctgc tgggcatcga cggcgggggc agcggcacgc gcgtcgtgct ggcccggcgc
     3241 gacggccccg tgctggggcg tggcgaggcc gggccgtcgg cgctggggca ggggatcgcc
     3301 caggcctggc gcaacgtcga acgggcctgc cgcgccgcat tcgacgccgc ccgcctgccc
     3361 gtgccgccct gggaccgctg cgcagccggc gccggcctgt ccggtatcag caaccggccc
     3421 tggcgcgacg ccttcgtggc cgccaacatc ggctttgccc gcctggaagc cgagaccgac
     3481 tccttcgtca tgctgctggg cgcccatcgc ggcaagcccg gtgccatcct ggccgtgggg
     3541 acgggcagtg tcgccgaggc gctgcgaccg gacggcagcc ggcacaccgt cggcggctgg
     3601 ggatttccgg ccggcgacga aggcagcggc gcgtggctgg gcttgcacgc ggtgcgccac
     3661 gcgcagtgcg cgctggacgg acgcgtcaac gccgggccgc tggcgcgccg tgtctgggcg
     3721 ctttgcggcg acgagcgcga tacgctgcag gactggtgcg acggggctgg ccagtttgcc
     3781 tatgcccagc tcgcgcccgc ggtgttcgag tgcgagggca ccgaccccgc cgccgcatcg
     3841 cttctgctcg aagcaacagc cgcgctggaa acgatggccc tggcggtgga tccgaaaggc
     3901 cgcctgccgc tggccgtctg tggcagcata ggactgcgcc tggcgccgcg tctcgcgccc
     3961 gccgtgcgaa gccggctggt accggcggca cagggccccg cggcaggggc cttgatgctg
     4021 atccggcagg ccgcccacgc tgaaatggaa gaactggtgt gagtctcaca ttgagcggcc
     4081 acatcctggg gccgcaaggg ttcgtcgcgg gaacgatgga actcgacggc tcggggcgcg
     4141 tgcgtcggat cacgggcgcg ccggtggccg cgagcgcctt gcgcgattca ggccagccgc
     4201 tggtgctgcc cggctttatc gacctgcatg tgcacggcgg tggcgggcgc gacatcatgg
     4261 agggcagcgg cgcggcgtcc cgggtcgcgc agtgccacgc cgcccacggc acgacggcac
     4321 tgttggccac caccatgacg gcgcccatgg ccgacctgga aaccgcgttc accggcctgc
     4381 gctccagctg tgacgagcgc ccggcgcgcg gcgcccgggt gctgggcgtg cacctggagg
     4441 ggccttacat caatcccggc aagctgggcg cgcagcccga tttcgcgcgg ccggtgtcca
     4501 tggaggaact ggcgcggctg gacgcgatcg cgccgatccg gctgatcacg ctcgcaccgg
     4561 aaatgccggg caacatggaa caggtcaagg cgctcagcga ggcgggctac cacgtccagc
     4621 tgggccacac gctgggcagc tacgaggatg gcgtccaggc gctggcactc ggtgcgcgcg
     4681 ggttcacgca cctgttcaac gccatgacgc cgctgcacca ccgcgcgccc ggcatggtgg
     4741 gcgccgcgct ggcgcacgcg cggtatgcgg agatcattcc ggacctgctg cacgtccacg
     4801 cgggcgcgct gcgcgtggcg ttgcggtcga ttccctgcct gtactgcgtc accgactcga
     4861 cggccgccac cggcatgccc gatggcgact accggctggg ccgccagacg gtgaccaagt
     4921 gcctgggcgg cgtgaggctc gccgacggca cgctggccgg ttccacgctg accatggacc
     4981 aggcgctgcg caacctggtc gatgagctgg gcctgtccct ggccgaagcg tccatgcgcc
     5041 tgtccaccca cgcggccgac tacctcggtc tggccgaccg cgggcggctg gcggcgggcg
     5101 cctgggccga cgtggtggtg ctggaccgcg acctgcaact gacggacgtg ttcgtcgaag
     5161 gagtgtcgac gtgagttcgc gcatgctggt tgaagcgtgc gaggccccgg ccgccgtggc
     5221 gcaacagctg gcccagggca gctcggccta ccgcgacctg ggagccttgc tgcgctccca
     5281 gccgcccacg tcgctgctca cggtggcccg cggcagctcg gactatgcgg cgaattacgt
     5341 cggatatctg atcatggccc gcatggggcg gctggtgact tcgctgccga tgtcgctggt
     5401 cacgctgtac cagtcgaaga tcgcctgccg cggcctcgct tcgttcgcct tttcgcagtc
     5461 gggacagagc ccggacctgg tcgagccgac gcgctatttc cgcgagggcg gcgcgatgac
     5521 ctcggccttc gtcaacgacc cggcctcgcc gctcgcgcgc gcggcgcaat gggtgttccc
     5581 cttgcatgcc ggcgtcgagt ccagcgtggc ggcgaccaag agcgtcattg cgcagatgtg
     5641 cgccggtgcc aggctggtgg cggcgtggca ggacgatggg gccctgcaaa cggcgctgtc
     5701 cgccttgccg caggtgttgt cccgggcggc gggcgcggac tggtcggccg ccctggacgt
     5761 cttgaaggat gccgaccggc tgctcgtgat cggacgcggg ctgggcctgc cggtggcgct
     5821 ggaagccgcg ctcaagttca aggaaacctg cgccgtccag gccgaggcat tctcgggcgc
     5881 cgaggtccag cacggcccca tggcgctgat cgaggaaggc tacccgctgc tcgtgttcgc
     5941 gccgcgcggg ccggcccagc ccgggctgct ggcgctcgcc gacgccatgc gttcgcgcgg
     6001 cgcgcgggtg ctgctcgcgg cgccggcggg cacaccccgt accagcctgc cgatcgcgga
     6061 aaccgcgtcg gccgagctcg acccgatctc catgctccag agcttctatc ccatggtcga
     6121 ggcgctgtcg cgctcacgcg ggctcgatcc cgaccagccg cgccacttga gcaaggtgac
     6181 ccgcacgcaa tgaatcgcaa gcgagtcctt gcggtgggtc agttcggtgc gtgggcgttg
     6241 ctgtatcctt gatcgcacag gaaaaaagga gtcgttgaac catgaaagcc ttgctggtga
     6301 cctgcatgct ggctgcagcc ccattcgtat gggcgagcga caagaccgcc ccccgagccc
     6361 cggcagccac cgctgtcacg ggcgaggttc tcgagatcca gaacgtcgag agctatacct
     6421 acctgcgcct gaagaccgct cagggcgaaa cctgggccgc tgtcaccacg gcgcccgtga
     6481 agaagggcgc caaggtcacg atcgaaaacg cgaccgtcat gaacaatttc gagagcaaag
     6541 cgctcaagaa gacgttcccg tcggtggttt tcggaacatt ggccggcgcg caaggcgtgg
     6601 cgggagccgc tgcaggcgcg gcggggcccc actcgggggc cggcaagccg aaggaaacag
     6661 ccgctgacgt ccacgttccc cgggccaccg gtgccaatgc ccgtacggtc gccgagatca
     6721 tgacgaaacc cgccgcattg aaggacaagg cggtgctggt gcgcggcaag gtcgtgaagt
     6781 acaaccccgg aatcatgggc aagaactggc ttcacctgcg cgacgggtca ggcaccgccg
     6841 ccgccgggac gaacgacgtg ctcgtcacca cgaccgagca agcgaaggtc ggcgatatcg
     6901 tgacggccaa gggcgtgctg cgcaccgacc gggacttcgg tgcgggctat tcgtacaagg
     6961 cgatcgtcga ggaagcgacg ctgcagcggt gatccctagt cagctgcgga cagagctaaa
     7021 ggtctgtccc gcaaggcttc agggccggca cggccaggcc gggggcgcat gatcgcccag
     7081 cctgatcgac agggctttga cgcgttcggc tgccgccagc cagcgtgcgt aggccaggac
     7141 gcgctcaacc gcacccaagc tggcgtcgct cgcgaccttc cactcggcgc gttcctgcgc
     7201 caccgcagcc gtgtactcgg cctgcagcga cttgtaggaa tcacggtcag tgctcatgtc
     7261 ggggcgatcc tacttgctgg aggcggtcct gtctgtgatt tattcctcgg caagccattg
     7321 ttcaactcgc cgcgatcagc cgaatcaaac cgaggttcta ctttcgcgtg tagatctggt
     7381 cgaaactgcc accgtcggtg atgtgggctt tgtcggcctt tgcccaaatg gcgagccctt
     7441 tccctggggg aggatcagcc gatccgcagg ttgccgacca ggtcggcgat cgagccgccc
     7501 acttcctcca ggcggcggcg cgcttcttcc gcgcccagcg acagcttcga aatgacgatg
     7561 gcggtcttca catgcccgcc cgacgcgtcc agcagccggc tggcttcctc gcgcttgacg
     7621 ccgagcgtct ccatcaggat gcgctcaccg cggtcctgca gcttctggca ggtgacctgc
     7681 agatcgacca tcaggtttcc gtagaccttg ccggtcttca ccatggccgc ggtgctgatg
     7741 gtgttcagga tgatcttggt ggcggtgccc gccttcatcc gggtcgagcc ggtgatgacc
     7801 tcggggccca ccagcggagc gatcaccacg tcgtgcgacg cgatgagccg ctcggacgga
     7861 taggtgcaga gaagaaagcc ggtacgggcg ccgcgttcgc gcgcgcgttt cagcgcgccg
     7921 tgcacgtagg gagtggtccc cgaagcggcg atgcccagga cgaaatcgtt ctcgccgatg
     7981 ttcttctcgt cgatggccgc cgcgccgtct tccggatggt cctccgcgcc ctcctgagcg
     8041 cgcagcagcg cgtcggtgcc gcccgcgatg atgccctgca ccatctccgg ctcggtgcgg
     8101 tacgtgggag gcatctccga cgcgtcgagc acgccgagcc ggccggaagt gccggcgccg
     8161 acatagacca ggcggccgcc cttgcgcaag cagtccacca ccaggtcgat ggcgcgcgcg
     8221 atctgctgcc gttcctcgcc caccgcggcg gcaaccatgc ggtcctccgc gttgatgagg
     8281 tcgacgattc cgatcgtgga cagctggtcg atgcgggtgc tacgcggatt gcgctgctcc
     8341 gtcaggcgag cgtccagggg cttggcaggg gtcatgggct tgcgggcggg cttggccgcg
     8401 ggttgcgaga gctccagctt cattgccgca cgctcctcca gatgcacgtt gctgcggtgc
     8461 cggtcctggc ccggcgacct ccttttctcg tccgacatgg gatgctccaa tactagaggt
     8521 accttgccat cgtcctccca cgatgccgac ttcattgtag gaccggcgtt gatttacctg
     8581 tacggtacat gcgcgacctg gacagggccg cgcaaggttg actgacaacc agccgatcat
     8641 caccggctga gtgattgccg gctaccagct atccacgtac cccgtcgccg gcggctcctt
     8701 gtgggtcgtc gccgccagag cgtgcaccag ccagctgcgc gtggcggccg gatcgatcac
     8761 ggcgtcgatc tccagcgtgg cggccatgtt catggcctgg ccgttttcgt actgccgggc
     8821 caccagttcg cggtacagcg cgtcgcgctg ctcgccttcc ggcgccgcct ccagttcctt
     8881 gcgatagccg agcttgacgg cgccctccag gcccatcgcg ccgaactcgc cggtcggcca
     8941 tgacaccatc gagtccggcg catggaagcc gcccgcggcc atcgccatcg cgcccaagcc
     9001 atagcccttg cgcagcacaa cgccgaagaa cggcacccgc aggtgggccg ccgcgacgaa
     9061 catgcggctg acgtgccgca cctgcgcttg ctgctcgatg tcgggcccga ccatgaaacc
     9121 cggcgtgtcg atcaggctca ccagcggcag gccgtgcacg ctgcaaagct gcatgaaacg
     9181 cgccgccttg tcggcggcgt cggcgtcgat ggctccgccc agatgcagcg ggttgttggc
     9241 cagcaggccg atcgggcggc cttcgatccg cgccagcgcc gtgtggatgc ccttcccgaa
     9301 accggtgcga agttccagca ccgagccggt gtcggccagg ccgctcatcg ccgcgcgggt
     9361 gtcgtagacg cgcagccggt tttcaggcac gacgtcgcgc aggctgtgcg gcgccggcgc
     9421 gcaaaacgat gtggccgctc cctggaagaa ggacaggtaa tgccgtgccg cgccgacggc
     9481 cgcggcctcg tcctccacca gcacgtcgat gacgccgttg ccgtgctgca cggcgctcgg
     9541 gccgatctgc tccggcttga aaacacccag gcctccgcct tcgaccatgg ccggcccgcc
     9601 catgccgatg ttgctggccc gggtggcgat gatcacgtcg ctgcacccca gcagcgccgc
     9661 gttgccggcg aagcagcggc ccgagacgat gcccaccacc ggcgccacgc cgttcatgcg
     9721 tgcgtagccg gcgaatgtgg cgacgtgcag gccggccacc acgggcatgt cggtatcccc
     9781 gggccgcccg cccccgcctt cggcgaacag caccaccggc aaccggttct tcagcgcgat
     9841 gccgagcatg cggtcggtct tctggtggtt gcgcatgccc tgcgtgccgg ccagcacggt
     9901 ggcgtcgtag gccatcacca cggcgcgcga gcgctccggc ccgaacagtt cgccgttgat
     9961 gctgccgatg cccgtgacca tgccgtccgc cggcgtgttg cgcctgaggt cttcctcgct
    10021 gcggcggctg cgctgcgccg ccacggccag cgcgccgtat tcgatgaaag tgccggggtc
    10081 gcacaggtcg gcgatgttct cgcgcgcggt gcgctggccg agctggtggc gccgcgcgac
    10141 ggcgtcgggg cgggcggcat cgagcacgag cgcgtggcga tcgatcaccc gctgcaggtc
    10201 ggggcggtgc gcctggccgc ccggtgccgc attgtcagcg cctttttgcg ccgctgccgc
    10261 cggtgccggg gcggccgcgc ccgccgggat cggctgcgtg accatcagca cgtcgtcctt
    10321 gtccaccgtg tcgccggcgg cgaagaacag ttcgcgcacc tggcccggcg cgccggcgcg
    10381 gacctcatgc tccatcttca tcgcttccag gatcaccacc acgtcgccgg cgcgcacgct
    10441 gtcgccggga ccgaccagcc attgcacgag ctgggcctgc agcggagcgc ggagttgggt
    10501 catccggcga ttgtggatca gctccaggca caggccgcgt caaccgggtg acaatgcggg
    10561 gcatgccttc gctggtgccc ctggtcgaac tgctgcgcgg cggcacgctg gagtgcgtgc
    10621 acttcggcgc ggtggctgtt tccgacgccc agggcaagct cgtcgcgagc gcgggcgatc
    10681 cgcactgggt cagcttctcg cgctccacgc tcaagccgct gcaggcgctg ccgttcgtgc
    10741 agggcggcgg tcccgggcag cttggctttt cgcgcgagca ggtggcgatg atgtgcgcca
    10801 gtcacagcgg cgagcccatg catgtggccc aggtcgaagc catgctcgac aaggcgggcc
    10861 tgagctacag ggcgctccag tgcggatgcc atatgccctt ctttgccgag ctcggcgcct
    10921 ggccgccgcc ggcgccgggc agctacgacg agcgccatca caactgcagc ggcaagcatt
    10981 ccggctttct ggcgtgttgc gtgcagcacg gctggcctgt agcaaactac cttgcgcccg
    11041 cgcatcccct gcagcaggcg atccggcgcg atgtcgcgcg agccgtgggg cttgcggccg
    11101 aggacgtgaa gatgggcatc gacggctgct cggcgcccaa ctacgccatg cccctcgtga
    11161 atctcgcgcg cggctatgcg cgcctggccg gcggcgcggc ggacggcgag ttcggcgcga
    11221 gcttcgcgca attggcggac gcgatgacgg cccatccgga gctggtctcc ggcaccggcc
    11281 gcaacgacct ggccttcatg cgtgccggcc gcggcgactg gatctccaag ggcggcgccg
    11341 agggcatgca cgccctggcc agccggagcc gccgccaggc cctggtcgtg aaggtcgccg
    11401 atggcaacaa gatcgcggtg caagccgcca ccgtggcagt gctggaccag ctcggctggt
    11461 tggacgcgcg ccagcgcgag gagctgcggc cgtggcgcgc cgaagcgatc gcgaacgccc
    11521 gaggcacgca ggtcggcgag c
//
LOCUS       DASWIY010000109         4500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2655412, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000109 DASWIY010000000
VERSION     DASWIY010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2655412"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VGA59_04250"
     CDS             <1..547
                     /locus_tag="VGA59_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9718920.1"
                     /translation="PTAAVLDAQSTRSSPQGGEHGFDAGKKVKGRKRNLVVDTLGLLL
                     AVTVTAASVQDRDAAHAVVGAAKEKYPSMQTLFVDGGYAGQCAQALRTENSLTVEVVR
                     HPANGNGGSWHHPGQGQLFPIKADTNGFVVLPKRWVVERTHDWNERARRLIMHHDRLA
                     RVSEAWVWLAEARLLLRRLAA"
     gene            835..2241
                     /locus_tag="VGA59_04255"
     CDS             835..2241
                     /locus_tag="VGA59_04255"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HEX9718921.1"
                     /translation="MPANAPAAVYQLKLRLRHTKPAIWRRIRLAGNTTLEDLHYIAQI
                     SMGWSNSHLHMFVTDSGQCFGSPEQDMDDLLEMLDERGMTVAELLRQPNQRLTYEYDF
                     GDGWEHEVLLEKILPPLDEEPLPRCVHALGKCPPEDVGGIGGFYTFLQAMRDPRHPEH
                     EDVLAWWGEPEFDPDEVDVDAINQRLQTPDEIVQQWEVDEGALDDFHGLNPDRMHQLL
                     YTPFDSPQCLQWRESEHAAQAPILRMAKVLLSTLADKEVKLTPKGNLPVALVKAMLAA
                     VDEQQPLLGFPHPHERRYAEENVMPVHATRLLVQLAGLTKTQKGKLSLKKTSTTDVTR
                     ERWGKVYLKMLKAMMLAFNWAYLDGYAGLRGVQTTSPFTLWLIHRHGGDWQPESFYSG
                     AILQAFPMLMAQASNESHAIAGETLEQLISWRTFKLYQWFGLIELREQASECRGFPYR
                     GPLSLRATKLFDAVLAFA"
     gene            complement(2422..3405)
                     /locus_tag="VGA59_04260"
     CDS             complement(2422..3405)
                     /locus_tag="VGA59_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX9718922.1"
                     /translation="MQGTTALRLGRNLAIVLALAGLSGWLVFVPSAQEPPYEFVGAWG
                     EPGSAPGQFNDPTGIAVAGDELFVADSRNGRIQVFDFAGTFKRQFGTPGSGAGQLGRP
                     MNLAIHAGELYVAEYYHDRIQVFGLDGTPRRIIGTSGSGPGEFDAPGGVAVAANGELW
                     VADVYNQRVQQLQADGRFVRQWGSTGRIGIRAGEFNYPTDVAIGPAGAVYVADGYNDR
                     IQAFDRQGAFVRKWGGPFASNIFGPFNGWFATVTAVAVDRQGNLFAADFYNHRIQKFS
                     ADGTFLTSFGSQGRGPGQFEHAMAVTVADDGTLFAVDFANSRVHKWRPAGP"
     gene            complement(3413..4294)
                     /locus_tag="VGA59_04265"
     CDS             complement(3413..4294)
                     /locus_tag="VGA59_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX9718923.1"
                     /translation="MQLAVSGLCLVAGATWGAPITFNTALPVHEGGYVWREQFLFMHS
                     ADDPTSARRDLQVTGLVSVLGYGVTRDFALLGILPWFDKRLGMRPDGQRLSRSQAGIG
                     DLTLLGRYTAYQYDAPGRTLRIAPFLGVKAPTGEDDARDRLGRLPPPLQPGSGSWDAL
                     GGAVLTYQTLAFQLDSQLSYRANRAANGFQAGNLWQVDGSLQYRLWPRCLGAGVPAFL
                     YGVLEVNLLHAAKDRSGGVSDANSGGSTLFISPGLQYVTRKWILEAGVQIPVRQQLNG
                     SALETDYVFTSGFRVNF"
     gene            complement(4303..>4500)
                     /locus_tag="VGA59_04270"
     CDS             complement(4303..>4500)
                     /locus_tag="VGA59_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718924.1"
                     /translation="KLNAIAGVEHLETRIEDGSVRVTMADGMSLDEAAAREAVTAAGF
                     SLRGFERLQSAAPGTPEGDAQ"
BASE COUNT          829 a         1466 c         1465 g          740 t
ORIGIN      
        1 gcccacggcg gccgtgctgg atgcccaatc cacgcgcagc tcgccgcaag gcggtgaaca
       61 cggcttcgat gccggcaaga aggtcaaggg gcgcaagcgc aacctcgtgg ttgacaccct
      121 gggcttgctc ctggcggtga cggtcactgc ggccagcgtg caggaccgcg atgccgctca
      181 cgccgttgtg ggcgctgcca aggaaaagta cccctcgatg caaacgctct ttgtcgatgg
      241 cggctatgcc ggacagtgcg cgcaagcgtt gcgcaccgag aacagtctca ccgtcgaagt
      301 cgtccgccat ccggccaatg gaaacggcgg ctcctggcat cacccaggcc agggccagct
      361 gttccccatc aaggccgata ccaacggctt cgtcgtgctc cccaaacgct gggtcgtcga
      421 acgaactcac gactggaacg agcgggcgcg cagactcatc atgcatcacg accgtctcgc
      481 gcgcgtctcg gaagcctggg tctggcttgc agaggcccgg cttctcctgc gccggctggc
      541 ggcctgatta ttttgtctac accctctcag aggttgtgaa aataccgaac gcaacgccgc
      601 ccgagccgcc gaatttgaaa caatcgcagc agtaggcgcg agcttattca caagctctca
      661 gcatcagcat cagcatcagc atcagcatca gcatcagcat cagcatcagc agcacgccga
      721 agccggtgga atgccagtgg cggcaagcgc attgcaaaac cggaggtcat ccaggcggtc
      781 ttgccctacc atttacaacc tgctccacct ctcccaggtc accaggattc ttgaatgcca
      841 gccaatgctc ccgccgccgt ctaccagctc aagctcaggc tccgtcatac caagcccgcc
      901 atctggcggc gaatacggct cgccggcaac accaccctcg aggacctgca ttacattgcg
      961 cagatcagca tggggtggag caacagccac ctgcatatgt tcgtcaccga cagcggccag
     1021 tgcttcggca gcccggagca ggatatggac gacctgctgg aaatgctgga tgaacgcggg
     1081 atgacggtgg cagagctgct gcgccagccc aatcaacgcc tgacctatga atacgatttt
     1141 ggcgatggct gggagcatga ggtgctgctg gaaaaaatcc tgccaccgct ggatgaggag
     1201 ccactgccca ggtgtgtgca tgcgctgggc aagtgtccgc cggaagatgt gggcggcatt
     1261 ggcggtttct acacctttct gcaggcgatg cgcgatcccc gtcaccccga gcatgaggat
     1321 gtcctggcct ggtgggggga gccggagttt gatccggacg aggtggacgt ggacgccatc
     1381 aaccaacgac tgcagacgcc cgacgagatc gtgcagcagt gggaggtcga cgaaggcgcg
     1441 ctggacgatt tccacgggct gaacccggac cgaatgcatc agctgctcta cacgccgttc
     1501 gacagcccgc agtgtctgca atggcgggaa agcgagcacg cggcgcaggc cccgatcctg
     1561 cgcatggcga aagtgctgct gagcacactg gccgacaagg aggtcaaact gacccccaag
     1621 ggcaacctgc cggtggctct ggtgaaggcc atgctggcgg ccgtggacga acagcaaccg
     1681 ctgctgggct ttccgcatcc gcacgagcgc cgatacgccg aagagaacgt catgccggtg
     1741 catgcgaccc gcttgctggt gcaactggcg ggcctgacga aaacccagaa gggcaagttg
     1801 tccctgaaga agaccagcac caccgacgtc acccgggagc gttggggcaa ggtgtacctg
     1861 aagatgctca aggccatgat gctggccttc aactgggcgt atctggacgg ctacgccgga
     1921 ctgcgaggcg tgcagaccac gtcgcccttc accctgtggc tgatccatcg ccatggcggc
     1981 gactggcagc cggaatcgtt ttatagcggg gcaatactcc aggcctttcc catgctcatg
     2041 gcgcaagcga gtaatgagtc ccatgccatc gccggcgaaa ccctggagca gctcattagc
     2101 tggcgcacct tcaagctgta ccagtggttt ggcctgatcg aactccgcga acaagcctcc
     2161 gaatgccggg gcttccccta cagggggccg ctgagcctgc gcgcgaccaa gctgttcgac
     2221 gcggtgctgg cgttcgcctg agggggtgtc gatcattcgg ggcatgcgtc gatgctcgtc
     2281 gaacaccgcc tgtcctctat cgggttcggc caggccctgg cgccgatcca aacgcaatgg
     2341 cgagcctggc cgaaactgag gcgagcgccg atccttcgcg tgggctcgcc ggcgggctcg
     2401 cgcatttccc acttccagcc gtcaaggccc cgcaggccgc cacttgtgca cccggctatt
     2461 ggcgaaatcg accgcgaaca gcgtgccatc gtccgctact gttactgcca tggcgtgttc
     2521 gaactggcct gggccacggc cctggctgcc gaaactggtg agaaaggtcc cgtccgcgga
     2581 aaacttctga atccggtggt tgtagaagtc ggcggcgaac agattgccct gtcgatcgac
     2641 ggccacagcg gtcacggtgg cgaaccagcc gttgaacggg ccgaagatgt tgctggcgaa
     2701 ggggccgccc cacttgcgca cgaaagcgcc ctggcggtcg aaggcctgga tgcggtcgtt
     2761 gtagccgtcg gcgacgtaga ctgcccccgc ggggccgatg gccacgtccg tggggtagtt
     2821 gaactcaccg gcgcggatgc cgatccggcc ggtgctgccc cactgccgga cgaagcggcc
     2881 atcggcctgc agttgctgaa cgcgctggtt gtagacatcc gcgacccaca actcgccatt
     2941 ggccgcgacc gccacgccgc cgggggcatc gaactcgccg gggccgctgc ccgaggtacc
     3001 gataatgcgc ctgggcgtgc cgtccaggcc gaacacctgg atacggtcgt ggtagtattc
     3061 ggccacatag agttcgccgg catggatcgc caggttcatc ggccggccga gttgcccggc
     3121 tccgctgccg ggcgtgccga actggcgctt gaaggtgccg gcgaaatcga agacctggat
     3181 acgcccgttg cgcgagtcgg cgacgaacaa ctcgtcgccg gcgacggcga ttccggtcgg
     3241 gtcgttgaac tgcccgggcg cgctgcccgg ctcaccccag gcgccgacga actcataagg
     3301 cggttcctgg gccgagggca cgaagaccag ccagccggac aggccggcga gcgccaggac
     3361 gatggcgaga ttgcggccca gtctgagggc ggtagtgcct tgcatggccg cctcagaagt
     3421 tgacgcgaaa gccgctggtg aagacgtaat cggtctccag cgcgctgccg ttgagctgct
     3481 ggcgcacggg aatctgcacg ccggcctcga ggatccattt gcgggtcacg tattgcagtc
     3541 cggggctgat gaacagcgtg cttccccccg aattcgcatc ggagacccca ccgctgcgat
     3601 ccttggccgc gtgcagcagg ttgacctcga gcacgccata gaggaaggcc ggaacccctg
     3661 cccccaggca gcgtggccat aggcggtact gcagcgagcc gtcgacttgc cagaggttgc
     3721 cggcctgaaa gccgttggct gcgcggttgg ccctgtagct cagctggctg tcgagttgga
     3781 aagccagggt ctggtaggtg agcaccgcgc cgcccagcgc atcccaggat ccggaacccg
     3841 gctgcagcgg cggtggcaga cgtcccagac ggtcacgggc gtcatcctcg ccggtcggcg
     3901 ccttcacccc gagaaagggc gcgatacgca aggtgcgacc gggggcgtcg tactggtagg
     3961 cggtatagcg gcccagcagg gtgagatcgc cgatccccgc ttggctgcgg ctgagcctct
     4021 ggccgtccgg gcgcatgccc aggcgcttgt cgaaccaggg caggatgcct aacagcgcga
     4081 aatcccgggt cacgccatag cccagcaccg agaccaagcc ggtcacctgc aggtcacgcc
     4141 tggcggaggt gggatcgtcg gcgctgtgca tgaagaggaa ctgctcgcgc cacacgtagc
     4201 cgccctcatg caccggcaac gccgtgttga aggtgatggg cgcgccccag gtggcgccgg
     4261 ccaccaggca caggccgctt acggctagct gcatggctgt cttcattgcg cgtccccctc
     4321 cggcgtgcca ggcgcggcgg actgcagccg ctcgaacccg cgcaggctga aaccggcggc
     4381 tgttaccgcc tcgcgcgccg cggcctcatc gaggctcatg ccgtccgcca tggtgacccg
     4441 cacgctgcca tcttcgatgc gcgtctcgag gtgctcgacg ccggcgatcg cgttgagctt
//
LOCUS       DASWIY010000110         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2656912, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000110 DASWIY010000000
VERSION     DASWIY010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2656912"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VGA59_04275"
     CDS             <1..285
                     /locus_tag="VGA59_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9718925.1"
                     /translation="AIRQVPGVWDCAVVGLPDERFGERPAAFIVAARDCDLDGPALTA
                     AVQRHCEEYLGRFKRPTTVQLLAALPRTPVGKLLRRRLREIATASQEQSQ"
     gene            282..1031
                     /locus_tag="VGA59_04280"
     CDS             282..1031
                     /locus_tag="VGA59_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX9718926.1"
                     /translation="MKILVPVKRVVDFNVKVRVKSDNTGVDIANVKMSMNPFDEIAVE
                     EAVRLKEKGVATEIIAVSCGPTQCQETLRTAMAIGADRAILVETTEELQPLSVAKILK
                     ALVDKEQPGLVILGKQAIDDDCNQVGQMLAALADLPQATFASKVEIADGQAKVTREVD
                     GGMETVSMGLPAVITADLRLNEPRYVTLPNIMKAKKKQLETFKPADLGVEIRPHLKTL
                     KVTEPPKRAAGVKVPDVATLVAKLKNEAKVI"
     gene            1188..2120
                     /locus_tag="VGA59_04285"
     CDS             1188..2120
                     /locus_tag="VGA59_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX9718927.1"
                     /translation="MTSLVVAEHNNASLKGATFNTVTAAAQCGGDVHVLVAGHNAGEA
                     AKAASQIAGVAKVIHAEGEHLAHGLAENMAAQVLALASAYSHILFPATASGKNIAPRV
                     AAKLDVGQISDVTRVDAADTFERPIYAGNAISVVQSLDEKKVITVRTTGFDPAAATGG
                     SAPIETVTATADSGKAKFLGNELAKSDRPELTAAKVIVSGGRAMGSSEKFNEVLTPLA
                     DKLGAALGASRAAVDAGYAPNDWQVGQTGKIVAPQLYIAAGISGAIQHLAGMKDSKVI
                     VAINKDPEAPIFSVADYGLEADLFVAVPELIKAL"
     gene            2261..>2775
                     /locus_tag="VGA59_04290"
     CDS             2261..>2775
                     /locus_tag="VGA59_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9718928.1"
                     /translation="MTYKAPLKDMLFDIRHLAGIDEIAKLPGFEEAGFETAQAVLEEC
                     ARFNEGVVAPLNVDGDRNPSSWKDGQVTTTPGFKEAYRQYTEGGWQGLQHPTEFGGQG
                     LPKTIGAACGEILNSANLSFALCPLLTDGATEALLTAGSEELNATYLANLISGKWTGT
                     MNLTEPQAGSDL"
BASE COUNT          546 a          915 c          918 g          396 t
ORIGIN      
        1 gccattcgcc aggtgcccgg cgtgtgggac tgcgctgtgg tgggtttgcc ggacgagcgc
       61 ttcggcgagc gccccgcggc tttcatcgtg gccgcgcggg actgtgacct cgacggcccc
      121 gcgctcaccg ccgccgtgca gcgccactgt gaagagtatc tcgggcgttt caagcgcccc
      181 accaccgtcc agcttctcgc tgcccttcct cgcacgcctg tcggcaagtt gttgcgccgc
      241 cggctgcgcg agatcgccac cgcctcccag gagcaatcac aatgaaaatc cttgtccccg
      301 tcaaacgggt ggtcgacttc aacgtgaagg tccgagtcaa gtcggacaac acgggggtgg
      361 acatcgccaa cgtcaagatg agcatgaacc cctttgacga gatcgccgtg gaggaggcgg
      421 tgcgcctgaa ggaaaagggc gtggccaccg agatcatcgc cgtgtcctgc ggccccacgc
      481 aatgccagga aacgctgcgc acggccatgg ccatcggagc ggaccgcgcc atcctggtgg
      541 aaaccaccga ggagctgcag ccgctgtcgg tggccaagat cctgaaggcg ctggtggaca
      601 aggagcagcc cggtctggtg atcctgggca agcaggccat cgatgacgac tgcaaccagg
      661 tcggccagat gctcgctgcc ctggccgacc tgccgcaggc cacgttcgcc tccaaggtgg
      721 agattgccga cggccaggcc aaggtcacgc gcgaagtgga tggcggcatg gagacggttt
      781 ccatgggcct gccggccgtc atcacggccg acctgcgcct caatgagccg cgctacgtga
      841 cgctgcccaa catcatgaag gccaagaaaa agcagctgga gaccttcaag ccggcggacc
      901 tgggcgttga gatccgcccg cacctgaaga cgctcaaagt gacggagccg cccaagcgcg
      961 ccgccggcgt caaggtgccc gacgtggcca cgctggtggc caagctgaaa aacgaagcaa
     1021 aggtgattta gcaagggctg tcatcccgga ccccggatca agtccggggc aggcccgatc
     1081 cgggatccat gctcgcgccg ccatggattg cgggtgctga aaccccggac ttgatccggg
     1141 gccgcaatga cagcatcgcc cagacagcca aagatacgga gaacctcatg acatccctcg
     1201 tagtagcaga acacaacaac gcctccctca agggagccac tttcaacacc gtcacagccg
     1261 cggctcagtg cggcggcgac gtgcacgtgc tggtggccgg ccacaacgcc ggcgaagcgg
     1321 ccaaggccgc cagccagatc gcgggcgttg ccaaggtgat ccacgccgaa ggcgagcacc
     1381 tcgcgcacgg gctggccgag aacatggccg cgcaggtgct ggcgctggcc agtgcctaca
     1441 gccacatcct gtttccggcc accgccagcg gcaagaacat cgccccgcgc gtggccgcca
     1501 agctggacgt ggggcagatc tcggacgtga cgcgcgtgga cgccgccgac accttcgagc
     1561 ggccgatcta tgcgggcaac gcgatctcgg tggtgcagag cctggacgaa aagaaggtga
     1621 tcaccgtgcg caccaccggg ttcgatccgg ccgcagccac gggcggctcg gcgcccatcg
     1681 aaaccgtcac cgccactgcc gacagcggca aggccaagtt cctgggcaac gaactcgcga
     1741 agtccgaccg gcccgagctg acggccgcca aggtcatcgt gtccggcggc cgcgccatgg
     1801 gctcgagcga aaagttcaac gaagtgctga cgcccctggc tgacaagctg ggcgccgccc
     1861 tgggcgcgag ccgcgccgcg gtggatgcgg gctatgcgcc caacgactgg caggtgggcc
     1921 agaccggcaa gatcgtggcg ccgcagctgt acatcgccgc gggcatctcc ggggcgatcc
     1981 agcacttggc cggcatgaag gactccaagg tgatcgtggc gatcaacaag gatccggagg
     2041 cgccgatctt ctcggtggcc gactatgggc tggaggcgga tctgttcgtc gccgtgccgg
     2101 aattgatcaa ggcgttgtag aaaccggtgt cattgcgggc ttgacccgca atccatgtgg
     2161 gcgcgccgac atggatgccg gatcgagtcc ggcatgacaa cccgtaggag acatggatgc
     2221 cggatcgagt ccggcatgac aagagccaca ggagaacccc atgacgtaca aagcccccct
     2281 caaagacatg ctgttcgaca tccggcacct ggcgggtatc gacgagatcg cgaaactgcc
     2341 gggtttcgag gaggcgggct tcgagaccgc gcaggccgtg ctcgaggaat gcgcccggtt
     2401 caacgagggc gtggtggcgc cgctgaacgt cgatggcgac cgcaatccct ccagctggaa
     2461 agacgggcag gtcacgacca cccccgggtt caaggaagct taccggcagt acacggaagg
     2521 cggctggcag ggcctgcagc atcccaccga gttcggcggc cagggcctgc ccaagacgat
     2581 aggcgcggcc tgcggtgaga tcctcaactc cgcgaacctg agcttcgcct tgtgcccgct
     2641 gctgaccgat ggcgcgaccg aagccctgct gacggccggc tcggaggaac tgaatgccac
     2701 ttacctcgcc aacctgatca gcggcaagtg gaccggcacc atgaacctga ccgagccgca
     2761 ggccggctcg gacct
//
LOCUS       DASWIY010000111         6161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_64160, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000111 DASWIY010000000
VERSION     DASWIY010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64160"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VGA59_04295"
     CDS             complement(<1..225)
                     /locus_tag="VGA59_04295"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX9718929.1"
                     /translation="MYKLVLVRHGESTWNLENRFTGWTDVDLTPTGIEQAKTCGRLLR
                     DEGWEFDLCYTSVLKRATRTLWHCLDEMDRT"
     gene            332..739
                     /locus_tag="VGA59_04300"
     CDS             332..739
                     /locus_tag="VGA59_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9718930.1"
                     /translation="MKFLIDNWMLISVAFASGAMLLWPVVQGGAAGGLSAAGAVQLIN
                     RERAIVVDVSESEEFAAGHVGGARNVPVGQLEQRLPEVVKNKGVPLILVCPSGIRAKR
                     ALAVAKKLGYDKAQVLAGGLKAWKDANLPVEKA"
     gene            751..1014
                     /gene="grxC"
                     /locus_tag="VGA59_04305"
     CDS             751..1014
                     /gene="grxC"
                     /locus_tag="VGA59_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX9718931.1"
                     /translation="MNPVKMYTTAVCPFCIRAKQFLLAKGVKIIEEIRVDMQPQERMR
                     MVQITGRRTVPQIFIGETHVGGCDDLMALDSRGGLKPLLEGAA"
     gene            1260..1730
                     /gene="secB"
                     /locus_tag="VGA59_04310"
     CDS             1260..1730
                     /gene="secB"
                     /locus_tag="VGA59_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX9718932.1"
                     /translation="MADQQQDPVFLIQRVYLKEASLEQPNSPGILLDQEQPAVDIQLA
                     VEASQAADGVYEVCVTATVTTKIKEKTVFLVEAKQAGIFEIRNLPPEQMSPVMGIACP
                     QIVYPYLRGNVADIITRAGFPPVHLAEINFQAMFEQQQAEQQQAAEPSRIITTA"
     gene            1770..2774
                     /locus_tag="VGA59_04315"
     CDS             1770..2774
                     /locus_tag="VGA59_04315"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX9718933.1"
                     /translation="MKILVFGAGAWGTALAVSAAPRHEVALWARDPAQASALAATREN
                     LRYLPGVALPAGVAVRADPAERLVDLARASELAIIATPMAGLRQLLCSLRGHLGPVAW
                     LCKGFEAPVAGGYGLLGHEIKAEVAPGLKAGILSGPSFAQEVARGQPTALVAASEHGE
                     VRDALVAAFHGDTLRVYANDDLPGVEVGGAVKNVLAIATGLCDGLNLGLNARAALITR
                     GLAEMTRLGVALGARPETFMGLSGLGDLVLTATGDLSRNRKVGLLLAQGQSAQQAVDS
                     LGHVAEGVYSARTVVLRAQHLGVDMPISRAVVALLDGRLRPAEAVSELMGRGPVAEAA
                     "
     gene            complement(2793..3122)
                     /locus_tag="VGA59_04320"
     CDS             complement(2793..3122)
                     /locus_tag="VGA59_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9718934.1"
                     /translation="MNSAYAETEPSRSEVDALTGPAVVEFGTPWCGYCRAAQPLIAGA
                     FASHPQVRHFKIEDGSGLPLGRSFGIKLWPTLVFLRDGKEVTRLVRPSGLDQVRQAME
                     RIDPAPA"
     gene            3245..3940
                     /locus_tag="VGA59_04325"
     CDS             3245..3940
                     /locus_tag="VGA59_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718935.1"
                     /translation="MNNKETRMRHSRVLMAASAAATVGLLVACAYSGAGEGGNPMSFF
                     ITSANPGKGADLGGLAGADRHCQALAAAVGAGGRTWHAYLSTAATASSPAVNARDRIG
                     RGPWRNVKGVVVAANVDELHGANKLNKQNSLTEKGGIIPGKGEPGIAHDILTGSTPDG
                     RAPAPGRDATCSNWTSSTEGGAIVGHHDRDGINPDPVANVSWNSSHPTPGCSPGALKR
                     VGGAGLLYCFAAN"
     gene            4011..4874
                     /gene="nadC"
                     /locus_tag="VGA59_04330"
     CDS             4011..4874
                     /gene="nadC"
                     /locus_tag="VGA59_04330"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX9718936.1"
                     /translation="MARTFDFSPEALAEVAGADAARALAEDVGPGDLTAGLVDPARRA
                     RARVLAREPAVICGAPWVEATLRQVEPGAHIHWLVPEGMRTLADQVVLEIEGSARGLL
                     TAERTALNFLQLLSAVATKTAAYVEAVRGTRAQIVDTRKTLPGLRLAQKYAVRTGGGT
                     NHRIGLYDAVLIKENHIAAAGGVKQVLAAAAQAAARAAFVEIEVETLAQLEEALAAGA
                     KMILLDNMELPLLREAVRINDARAMLEISGGVTLESVRALAETGVDRISIGALTKDVK
                     ATDFSMRLKEI"
     gene            4877..5995
                     /gene="nadA"
                     /locus_tag="VGA59_04335"
     CDS             4877..5995
                     /gene="nadA"
                     /locus_tag="VGA59_04335"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX9718937.1"
                     /translation="MSAVIPIYDVEYEHPLTGNTCDTKHGWARVPVEPSAAERAELKA
                     RIRRLLKERNAVMVSHYYVHPDLQDLAEETGGIVSDSLEMARFGRDHAARTLVVSGVK
                     FMGETAKIVSPEKRVLMPDLDATCSLDLGCPAGEFSAFCDQHPDRTVVVYANTSAAVK
                     ARSDWLVTSSCALDIVRALTEKGHKILWAPDKHLGTYIQRETGADMVSWNGSCIVHDE
                     FKAFELEALKKDYPRAKVLVHPESPADVVALADAVGSTSAILKAAREMDATEFIVATD
                     NGMMHKLRTLNPGKVFIEAPTAGNSATCKSCAHCPWMAMNGLAGLAHVLETGTNEVHV
                     DPALGQRARVPIDRMLAFTAALKAGQPAGSLVPNIGAA"
BASE COUNT         1058 a         2030 c         2110 g          963 t
ORIGIN      
        1 ggtgcggtcc atctcgtcca ggcagtgcca caaggtgcgg gtggcgcgtt tcaggacgct
       61 cgtgtagcaa agatcgaatt cccacccctc gtccctgagc agccggccgc aggtcttggc
      121 ctgctcgatc ccggtcggcg tcaggtccac gtcggtccag ccggtgaacc ggttttcgag
      181 gttccaggtc gattcgccgt ggcggacaag cacgagtttg tacatgggga aggccttgga
      241 atgcgtggtg caaaacccgg cattttagaa tggtgggttg gccggcgccc attgcaagcc
      301 ggccggccag gctctcacta aaaggaaaga cgtgaaattt ctcatcgata actggatgct
      361 gatctcggtg gccttcgcct cgggcgccat gctgctgtgg cccgtggtcc agggcggcgc
      421 cgctggcggc ttgtccgcgg ccggtgccgt gcagctcatc aaccgcgagc gcgcgatcgt
      481 ggtggacgtg agcgaatccg aggaattcgc cgccggccac gtgggcggcg ccaggaacgt
      541 gccggtgggg cagctggagc agcgcctgcc ggaagtggtc aagaacaagg gcgtgccgct
      601 catcctggtg tgcccttctg gcatacgtgc gaaaagggcg cttgccgtgg cgaaaaaact
      661 cggctacgac aaggcccaag tgctggccgg cgggctgaaa gcctggaaag acgctaacct
      721 tcctgtcgag aaagcctgaa cagcgagacc atgaacccag tcaagatgta caccacggcc
      781 gtttgcccct tctgcattcg cgccaagcaa ttcctcctgg ccaagggcgt caagatcatc
      841 gaagagatcc gggtcgacat gcagccccag gagcgcatga ggatggtgca gatcaccggc
      901 cggcgtacgg tgccgcagat cttcatcggc gaaacccatg tgggcggctg cgacgacctg
      961 atggccctgg acagccgggg cggcctgaag cctctgctgg agggcgcggc ctgaagtaaa
     1021 agcccacccc cgaagcgcct tacggccgac gtgccactgc cagaggcagt ggctcttcgg
     1081 gccgtccaaa cctgcgcagg caggcttgga gccgcggccc tcagccccct caaggggacg
     1141 ccaccagcgg cccggcaaag ccggttccgc ggtggccccc gcacggaccg cgcgataatt
     1201 caccctgacc cgaatcgggc ccgcttcggc gggcttttct attgacccga aagatcccca
     1261 tggccgacca gcaacaagac cccgtattcc tgatccagcg cgtgtacctc aaggaggcct
     1321 cgctcgagca acccaattcc cccggcatcc tgctggacca ggagcagccc gcggtggaca
     1381 tccagctggc ggtggaggct tcgcaggccg ccgatggtgt gtacgaggta tgcgtcacgg
     1441 ccaccgtgac gaccaagatc aaggaaaaga ccgtgttcct ggtggaggcc aagcaggccg
     1501 gtattttcga gatccgcaac ctgccgccgg aacagatgag cccggtcatg ggcatcgcct
     1561 gcccgcagat cgtctacccg tatctgcgcg gcaacgtggc ggacatcatc acccgcgccg
     1621 gatttccgcc ggtgcacctg gccgaaatca atttccaggc catgttcgag cagcagcagg
     1681 ccgagcagca gcaggcggcc gagccctcgc gcatcatcac cacggcttga cgccgaggcc
     1741 agggccgacg cgtcctggtc gacgccatta tgaaaatcct ggtattcgga gccggcgctt
     1801 ggggcacggc gctggccgtg agtgcggccc cccggcatga ggtggcgctg tgggcccgcg
     1861 accccgcgca ggcgagcgcg ttggcggcga cgcgcgagaa cctgcgttac ctgcccggcg
     1921 ttgccttgcc cgccggggtt gcggttcgcg ccgaccccgc cgaaaggctg gtcgacctgg
     1981 cccgggccag cgagctggcg atcatcgcca ccccgatggc agggctgcgc cagctgctgt
     2041 gctccttgcg cggccacctc gggcccgtcg cctggctgtg caagggattc gaagcgccgg
     2101 tggcgggcgg ctacggactg ctcggccatg aaatcaaggc cgaggtcgcc cccggcctga
     2161 aagccggcat cctcagcggg ccgagcttcg cccaggaagt ggcgcggggt cagccgacgg
     2221 cgctggtggc cgccagcgaa cacggcgagg tgcgcgacgc cctggtggcg gctttccacg
     2281 gcgataccct gcgggtctac gccaatgacg acctgccggg cgtcgaggtc ggcggtgccg
     2341 tgaagaacgt gctggcgatc gcaacgggcc tgtgcgacgg cctgaacctc ggactgaacg
     2401 cgcgggccgc gctcatcacg cgcgggctgg cggagatgac gaggctgggg gtcgcgctgg
     2461 gggcgcggcc cgaaaccttc atgggtcttt ccggcctggg cgacctggta ctgaccgcca
     2521 ccggcgatct cagccgcaac cgtaaagtgg gattgctgct ggcgcagggc caatcggctc
     2581 agcaggcggt ggattccctg ggccatgtgg ccgagggcgt gtacagtgcg cggacggtcg
     2641 ttttgcgcgc gcagcacctg ggcgtggaca tgcccatctc gcgcgccgtt gtcgcgctgc
     2701 tcgacggcag gctccgaccg gccgaagccg tttcggaact gatggggcgc gggccggtgg
     2761 ccgaggcggc ctgagcgccg ctggacatgc cctcaggcgg gcgcaggatc gatccgctcc
     2821 atcgcctggc ggacctggtc gaggccgctc gggcgcacca gccgggtcac ttccttgccg
     2881 tcgcgcagga ataccagtgt cggccagagc ttgatcccga aggagcggcc cagtggcaga
     2941 ccgctcccgt cctcgatctt gaagtggcgc acctgtggat gggaggcgaa agcccccgcg
     3001 atcaatggct gcgccgcgcg gcagtaaccg caccagggcg ttccgaattc cacgacagcc
     3061 ggcccggtca gggcatccac ctctgagcgt gatggttcag tttcggcata agcactgttc
     3121 atgagcgaca ttgtcccaaa tgagcggccc gcctcccgta ggccggggcc cccggggcgg
     3181 gtcgtcctga tcctggggcc tgttctcttg acaacgcgat gcgccgggat agtctgccag
     3241 ctccatgaac aacaaggaga caaggatgcg ccattcccga gttctgatgg ctgcctcggc
     3301 cgcggctacg gttggcctgc tggtcgcatg cgcctattcc ggcgctggcg aaggcggcaa
     3361 tccgatgagc ttcttcatca ccagcgccaa tcctggcaag ggggctgacc tcggcggatt
     3421 ggcgggcgcc gaccggcact gccaggcgct ggccgcggcc gtgggcgccg gaggccggac
     3481 gtggcacgcc tacctgagca cggcggccac cgcgtcgagc ccggctgtca atgcacgtga
     3541 ccggatcggc cgcggtccct ggcgcaacgt caaaggcgtc gtggttgcgg cgaatgtgga
     3601 tgagctgcac ggtgccaata agctgaacaa gcagaactcg ctgacggaaa agggcgggat
     3661 cattcccggc aaaggggaac cgggaatcgc tcatgacatc ctgaccggct cgaccccgga
     3721 cggccgggct ccggcccccg gccgcgatgc gacgtgcagc aactggacca gcagcactga
     3781 aggtggcgcc atcgtcgggc accacgaccg cgacggcatc aaccccgatc cggtggcgaa
     3841 cgtttcctgg aactcgtctc atccaacgcc gggctgcagc cctggggcgc tcaaaagggt
     3901 aggcggcgcc gggttgctgt actgcttcgc cgccaattga acggacgccg gctgcggcct
     3961 ctgtctgcgc gatatctccc gggttcgggg atatcgctat gcttgcctcc atggcaagaa
     4021 ctttcgattt ctcaccggaa gccctggctg aagtggccgg cgcggacgcg gcgcgggcgc
     4081 tggccgagga cgtgggcccg ggcgacctga ccgcgggcct ggtcgatccg gcccgccgtg
     4141 cccgggcccg ggttctggct cgggagcccg ccgtcatttg cggagcgccc tgggtcgagg
     4201 caaccctgcg ccaggtggaa cccggcgccc acatccactg gctggtcccc gagggcatgc
     4261 gcacactggc cgaccaggtg gtgctggaga tcgaaggctc ggcccgcggc ctgctcacgg
     4321 cggaacgcac cgccctcaat ttcctgcaac tgctcagtgc ggttgcgacg aagacggccg
     4381 cctatgtcga ggccgtgcgc ggcacgcgcg cgcagatcgt tgacacccgc aagacgcttc
     4441 cgggcctgcg cctggcgcag aaatacgccg tgcgcacggg cggcggcacc aaccaccgca
     4501 tcggcctgta cgacgccgtg ctgatcaagg aaaaccatat cgccgccgcc ggtggcgtca
     4561 agcaggtgct cgcggcggcg gcgcaggcgg ccgcgcgggc cgcgttcgtc gagatcgagg
     4621 tcgaaacgct ggcccagctg gaagaggcgc tggcggccgg cgcgaagatg atcctgctcg
     4681 acaacatgga gctgccgctg ctgcgcgaag ccgtccgcat caacgacgcc cgcgccatgc
     4741 tcgagatttc cggcggcgtc acgctcgaga gcgtgcgcgc ccttgcagaa accggtgtgg
     4801 accgcatctc cattggagcc ctcaccaagg acgtgaaggc gacggacttc tccatgcgtc
     4861 tcaaggaaat ctgaagatga gcgccgtgat ccccatctac gacgtcgagt acgagcaccc
     4921 gctgaccggc aatacctgcg ataccaagca tggctgggcc cgcgtgccgg tcgagccgtc
     4981 cgccgccgaa cgcgccgaac tcaaggccag gatccgccgc ctgctcaagg agcgcaacgc
     5041 ggtgatggtg tcgcactact acgtgcaccc cgacctgcag gacctggccg aagagaccgg
     5101 cggcatcgtc agcgattcgc tggagatggc gcggttcggg cgcgaccacg ccgcccggac
     5161 cctggtggtg tccggcgtga agttcatggg cgaaaccgcg aagatcgtgt cgcccgaaaa
     5221 acgcgtgctg atgcccgacc tggatgcgac ctgttcgctc gatctgggct gtccggcggg
     5281 cgagttctcg gccttttgcg accagcatcc cgaccgcacg gtggtggtct atgccaatac
     5341 cagcgcggcg gtgaaagcga ggtcggactg gctggtcacg tccagctgcg cgctggatat
     5401 cgttcgggcg ctgacggaga aaggccacaa gatcctgtgg gcgcccgaca agcatctggg
     5461 aacgtacatc cagcgggaaa ccggcgccga catggtgtcc tggaacggct cgtgcatcgt
     5521 gcacgacgaa ttcaaggcct tcgagctcga agcgctcaag aaggactacc cccgggccaa
     5581 ggtgctggtg caccccgagt cgccggcgga cgtggtcgcg ctggcggatg ccgttggatc
     5641 gacttcggcg atcctcaagg ccgcgcgcga gatggacgcc acggagttca tcgtcgccac
     5701 cgacaacggc atgatgcaca agctgcgcac gctcaacccc ggcaaggtgt tcatcgaagc
     5761 gccgacggcc ggcaacagcg cgacctgcaa gagctgcgcg cattgcccgt ggatggcgat
     5821 gaatggtctg gcgggcctgg cccatgtgct ggaaacgggc acgaacgaag tccacgtcga
     5881 tcccgccctg gggcagcgcg cgcgcgtgcc gatcgaccgc atgctcgcgt tcaccgccgc
     5941 gctcaaggcc gggcaaccgg ccgggtcgct ggtgccgaac atcggcgcgg cctgaaatgt
     6001 tgtcatccgg accccggatc aagtccgggg caggcctgat ccggcatcca tgcgttcggc
     6061 tgagatggat tgcggcacag ggccgcaatg acaatccttc acggaaccag gatcgtcggc
     6121 ttcgagtccg ggtccatctc gggatagtcg cggctgaaat g
//
LOCUS       DASWIY010000112         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3032459, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000112 DASWIY010000000
VERSION     DASWIY010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3032459"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VGA59_04340"
     CDS             <1..496
                     /locus_tag="VGA59_04340"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX9718938.1"
                     /translation="EMGSWVMTRERMRIPEGNCEFAINMSNQRHWAPPMQRYIAECLA
                     GATGPCGVDFNMRWTGSMVADIYRILKRGGIFLYPWDGRDPKKPGKLRLMYEANPMGF
                     IIEQAGGKAFDGHSRILDIEPTALHQRVGVAMGDRTEVERVVRYHREHESAARSDRVA
                     YPAK"
     gene            499..894
                     /gene="crcB"
                     /locus_tag="VGA59_04345"
     CDS             499..894
                     /gene="crcB"
                     /locus_tag="VGA59_04345"
                     /inference="COORDINATES: protein motif:HMM:NF010799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX9718939.1"
                     /translation="MTALHVLAVAIGGALGGTLRFWVSGVVARRFGERFPWGTLVVNV
                     SGAALIGAIAGLVLAPGSHSIGQPTVWAVLVVGILGSYTTVSSFSLQTLALLRSGEPG
                     RAAANVAASVGLCPAAAAASFTLVIWMGT"
     gene            931..1311
                     /gene="crcB"
                     /locus_tag="VGA59_04350"
     CDS             931..1311
                     /gene="crcB"
                     /locus_tag="VGA59_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX9718940.1"
                     /translation="MAAGSALGALARYLCSVAAVALLGPAFPWGTLTVNVLGSFLIGL
                     YASLSEPDGRLLASPAQRQFVMAGFCGGFTTFSIFSLETLLLVDRQEFHLAAIYVTVS
                     VALWLASVWIGYRIGSKMNRLKRS"
     gene            1316..1660
                     /locus_tag="VGA59_04355"
     CDS             1316..1660
                     /locus_tag="VGA59_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX9718941.1"
                     /translation="MQAYTEAVLLRMFIGESDRYDGQPLYEAIVLKARELGIAGATVL
                     RGPMGYGRSSRLHTTKILRLSDDLPLIIEIVDTKDKIDVLLEQAEPMLGSGLVTLENV
                     RVVRYGHDAGLN"
     gene            2010..>3278
                     /locus_tag="VGA59_04360"
     CDS             2010..>3278
                     /locus_tag="VGA59_04360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX9718942.1"
                     /translation="MPALSTYNLTGDPYIDGLLGHFKWAVTSFTYSSPGSASYYGTNY
                     GNGEPTDNFGTLNTTQRSMVDAAMSMYASVTGLSFSEITETNSQHADLRFAMSDKPST
                     AWAYYPSTAEEGGDAWFNNSSGWYDNPVKGNYAHSTFLHEIGHALGLEHPQDGNVMPL
                     DRDSLEYTVMSYRSFIGGSLTGGYTNETWGYPQSLMMYDIAALQHLYGPNYGTNGGNS
                     TYSWSPTTGEMFINGVGQGAPGANRIFLTVWDGGGTDTYDFSSYTTDLSVDLRPGEWT
                     TTSSAQLARLHYNGSEVATGNIANALLYNGDPSSLIENARGGSGNDVIVGNAAANTLE
                     GLDGNDEIWGGNGFDLLYGGDGRDRLFGGNANDRLFGGSGHDGLFGGNGHDVLSGGHG
                     YDHLYGGFGYDHLYGGYGNDRLAGGYGNDRL"
BASE COUNT          586 a         1020 c         1034 g          638 t
ORIGIN      
        1 ggagatgggg tcatgggtga tgacccgtga gcgcatgcgc atccccgagg gcaattgcga
       61 gttcgccatc aacatgtcga accagcgcca ttgggcgccc cccatgcagc gctatatcgc
      121 cgagtgcctt gccggcgcga ccggcccgtg cggggtcgat ttcaacatgc ggtggacggg
      181 ctcgatggtc gccgacatct accgcatcct caagcgcggc ggcatcttcc tctacccgtg
      241 ggacgggcgc gatccgaaga agcccggcaa gctgcgcctg atgtatgaag caaatcccat
      301 ggggttcatc atcgagcagg ccggaggcaa ggccttcgac ggccacagcc gcatcctcga
      361 catcgagccg acggccctgc accagcgggt gggggtcgcg atgggcgacc gcaccgaggt
      421 ggaacgggtt gttcggtacc atcgtgagca tgaatccgcg gcccgttccg atcgggtggc
      481 ctacccggcc aaatgagcat gacggcgctt catgtgctcg ctgtcgcaat cggcggcgcg
      541 ctcggcggga ccttgcgctt ctgggtctcg ggcgtcgttg cccgccgctt cggagagagg
      601 tttccatggg ggaccctcgt ggtgaacgtc tccggtgcgg cgttgatcgg cgcgatcgcg
      661 gggctcgttc tggcgcctgg gagccattcc atcggccagc ccaccgtttg ggcggtgctc
      721 gtcgtcggca ttctcggcag ctacacgacg gtatcctcgt tctcgcttca gacgctggcc
      781 ctgcttcgat ccggcgagcc gggcagagcc gccgcaaatg tggccgcatc ggtcggcttg
      841 tgtccggcgg ccgccgcggc atcctttaca cttgtcatct ggatgggaac ctgaacagtg
      901 gcgggggagc accgacgcct ttatgccgcc gtggctgcgg gcagcgcact cggcgcgctc
      961 gcgcgttacc tgtgcagcgt cgcggcggtg gcgctgctcg gaccggcgtt tccctggggc
     1021 acgctgacgg tgaacgtgct gggctcgttc ctgatcggcc tctatgcttc cctgagcgag
     1081 cccgatgggc ggctgctggc aagccccgct cagcgtcagt tcgtgatggc gggcttttgc
     1141 ggcggtttca ccactttttc gatcttcagc ctggagacgc tgctgctcgt cgatcgacag
     1201 gagtttcatc tggccgccat ctatgtcacc gtctcggtcg ccctgtggct cgcctcggta
     1261 tggatcggct atcgcatcgg cagcaagatg aaccgtctga agcggagttg aactcatgca
     1321 agcctatacc gaagctgtgc tgctgcggat gttcatcggc gaaagcgacc gttacgacgg
     1381 ccaaccgctc tacgaggcga tcgtgctgaa ggcgcgcgag ctgggtattg cgggagccac
     1441 cgttctgcgc ggccccatgg gctacggccg ctcgagccgc ctgcacacca ccaagatcct
     1501 gcggctgtcc gacgacctgc cgctgatcat cgagatcgtc gacaccaagg acaagatcga
     1561 tgttctgctg gaacaggccg aaccgatgct cggcagcggt ctggtgacgc tggagaacgt
     1621 gcgggtggtg cgctacgggc atgatgcggg cctgaactga cttgtcaggc ttcacatcgc
     1681 gcttggtact actataccac ctgtcgtgcg cggccacgtt ttggttcaca taatcgactt
     1741 ttttgtcggg gatgctggcg ctcgtttgat tcacaaattc tgcaattcat atcaaggtgg
     1801 ccgcttcgtg ttttggtttt cgtaaaccat atgaactagc cttcccagcc tattgtcatt
     1861 cgcccagatg tcaggcaaca ggccgaattg aggtagaaac gggaaagact acattgcggg
     1921 tcagcagttc ggctcagcca atcttcctga tgcggtcgat tgacctgagt gcagctcagc
     1981 cgccctcgtg aaccggaacg gatcgataca tgcctgcctt atcgacctac aacctcaccg
     2041 gagatcccta tatcgatggc ctcctgggcc acttcaaatg ggccgtaacc agcttcacgt
     2101 acagttctcc cggcagcgcc tcctattacg ggacgaacta cgggaacggc gagcccactg
     2161 ataacttcgg gaccctgaac accacccaga ggtcgatggt cgatgcggca atgtcgatgt
     2221 acgcctccgt caccggcctg agcttttccg aaatcaccga gaccaacagc cagcatgcgg
     2281 acctgcgctt tgccatgtcg gacaagccgt ccacggcctg ggcgtactat cccagcaccg
     2341 ccgaagaggg cggcgacgcc tggttcaaca actcgagcgg ctggtacgac aatccggtca
     2401 agggaaacta cgcgcattcg accttcctgc acgagatcgg tcacgcgctc ggtctggagc
     2461 acccgcaaga cggcaacgtg atgccgctcg accgcgattc gctggaatac acggtgatga
     2521 gctaccgctc gttcatcgga ggttcgctca ccggggggta caccaacgag acctggggct
     2581 atccccagtc gctcatgatg tacgacatcg cggcgctgca gcatctgtac ggaccgaact
     2641 acggcaccaa cggcggcaac tcgacatact cctggagccc cacgacgggc gagatgttca
     2701 tcaacggcgt tggacagggc gcgcccggag cgaaccggat ctttctcacg gtgtgggacg
     2761 gcggcggcac cgacacctat gacttttcca gctacacgac ggacctcagc gtggacctgc
     2821 ggccgggcga gtggacgacg acctcgtcgg cgcagctggc caggctccat tacaacggct
     2881 cggaagtcgc gacaggcaac atcgccaacg ccctgctgta caacggcgac ccaagctccc
     2941 tgatcgagaa cgccaggggc gggtccggca acgacgtcat cgtcgggaac gctgcggcca
     3001 acacgcttga ggggctcgac ggcaacgatg aaatttgggg cggtaacggc ttcgacttgc
     3061 tgtatggcgg cgacggtagg gatcgtctgt tcggcggtaa cgcgaacgat cgcctgttcg
     3121 gtggcagtgg ccatgatggc ctgttcggcg gcaatggcca tgatgtcctg tccggcggcc
     3181 atgggtatga ccacctgtac ggcggcttcg gttatgacca cctgtacggc ggctacggca
     3241 atgatcgcct tgccggcggc tatggcaacg atcgcctg
//
LOCUS       DASWIY010000113         9968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3032483, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000113 DASWIY010000000
VERSION     DASWIY010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9968
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3032483"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VGA59_04365"
     CDS             <1..759
                     /locus_tag="VGA59_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9718943.1"
                     /translation="SGIATLTLNRPDKRNAISDDMRTEFVDALERIGADKAIRALVVT
                     GAGKGFCAGGDIAGMERRMNAPAGEIAFNGWHRQQRVHHAQALLHTMPKPVIAAVNGA
                     ASGLGADTALACDFIIASERASFTWSYIHRGIVPDGGGMYFLPRRVGLPRAKDLIFTG
                     RKVDADEALALGIVDRKTTADRLVADAQAWAAELTKGSATALALGKTILNQAFELSAQ
                     QVFAQGSQAQGICYTSTEHRESVMAFLAKSTPTK"
     gene            765..2855
                     /locus_tag="VGA59_04370"
     CDS             765..2855
                     /locus_tag="VGA59_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX9718944.1"
                     /translation="MDAISRLLKPRSVAVVGASGDASKTAGRPVSYLVKHSFAGEIYP
                     VNPKADRIGKLACYPDVASLPAVPDVGIVLLGAERAHLAVRDLAARGCTAAIVLASGY
                     TETGEEGARRQKQLMEAAGSMRILGPNTIGLVNLTDNIVLSATGALEMDHFPVGSIGV
                     VSQSGGILGSLLSRAAARGIGLSKLVSTSNEVDLELADFIDALVDDAATKVIALYIET
                     VRNPAKFRAAALKAARAGKPIVAFKIGRSEAGAKAAVSHTGALAGADRMYDALFRQTG
                     VIRAQGFADLLDIPAALATGRVLRGNRVAILTSTGGAGTLVSDSLGVSGFDTPAPDAQ
                     TAAALRALQTGDHAALDRNPIDVTLAGLQPDLLRGAIKALLASPSYDALGIIVGSSGL
                     AMPELMADAIQDCLPLSDKPVIAYVSPHAPEAAALLTQRGVPAFAAAESCSAALAGML
                     QASRWKAPAVEAASESGIALDDLPAGSLDEAQAKQLFARFGIPCPREMVVETPAEGER
                     AARELGGRVVLKILSGEITHKSDVGGVAVNLAPEQVSARLSTMADEVETKAGVRPRRF
                     LVQEMVTGGTELILGMRRDPLGTAILLGMGGVAAELLKDTSMRLLPPEGGLSRDEALS
                     MAQELKTWPLLDGFRGRPKADVEALVSAIVGFSRMAAGLGERLVEAEINPVFVLPRGQ
                     GVRAADGVVVLRHK"
     gene            complement(2897..3244)
                     /locus_tag="VGA59_04375"
     CDS             complement(2897..3244)
                     /locus_tag="VGA59_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718945.1"
                     /translation="MLKTAVVLFALTAMGGLIMAFIRFSGSERPPSWLAMGHGLLAGS
                     GLTLVLYDAFTAGLPTLAWLGAGVLVVAAAGGAFLNLAYHAQLKALPKGIVVLHALLA
                     VAGFGLLALAAFR"
     gene            complement(3903..4061)
                     /locus_tag="VGA59_04380"
     CDS             complement(3903..4061)
                     /locus_tag="VGA59_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEX9718946.1"
                     /translation="MSLGTILLIVLVLMLIGVIPTWSHSSSWGYGPSGILGVVLIIVV
                     VLLLMGRL"
     gene            4291..4497
                     /locus_tag="VGA59_04385"
     CDS             4291..4497
                     /locus_tag="VGA59_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX9718947.1"
                     /translation="MNKDQVKGEIKEVAGKVQEKAGQVIGSTDQQIKGLAKQVEGKTQ
                     KAVGDVKEVVKEAVKDVTKDVAPK"
     gene            complement(4661..6316)
                     /locus_tag="VGA59_04390"
     CDS             complement(4661..6316)
                     /locus_tag="VGA59_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9718948.1"
                     /translation="MLQGFTGWPQAAAQRYREAGLWEGLTVSEMVARTARSQPDKVAV
                     VYGARRITYAELIAESRRLALGLIELGLKPLDRAVMQLPNIPEFVTTYLALNWIGVIP
                     VTALRAHRHAEVRHFIRASEATAYFISDTVGGFDYRPMAAEMSADFPFLRHVVVVGQA
                     GAGQTALDSHVAPAMHEEALASQLNPVRPAADEVSTMLLSGGTTSMSKLIPRTHNDYV
                     LNARLCAQAGGFDGDTVFMAVLPLGHNYNLASPGLLGAFYSGGTLVIATSTDTEEIFR
                     TVEREGVTVIAAVVPLISNWLNSEVPKRCDLSSLRVVQNGGARLAPELRARLRKEFEC
                     TPQEVYGTAEGLINMTRLDDPDELLLESSGAPVCDFDEIKVLDDAGEEVPDGEPGELV
                     VRGPYTINGYYNAPEKNAEAFTADGFYRMGDIVRKRGRYVYTEGRRKDMINRGGEKIS
                     CDEIENLIFGLPQVKQVSLVAMPDPVFGEKACACVVLKPGTRLAFDELIAHLRAQHIA
                     SFKLPERLEVMPSFPISPVGKILKRELRDMIASKVEAERASRG"
     gene            complement(6349..7482)
                     /locus_tag="VGA59_04395"
     CDS             complement(6349..7482)
                     /locus_tag="VGA59_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9718949.1"
                     /translation="MKIRIVGAGPAGLYFAALMKQHDPSHEITLFDRSARNATWGFGV
                     VFSDRALEFLRADDEALYRYLTPHLETWPDITIVHNDTRIPIAGNGFASIGRLELLTL
                     LYAYVEKLGLKINFETEVTSLEQLGDADLVVAANGAFSSIRGENEAKFGTTTQWRGNK
                     FIWYGTSKAFDSLTLTFRETDAGVFCAHHYRYRPDMSTFLVEVEDATWQRAGFKDMSP
                     EDTIKHCERVFARDLQGHPIISNNSYWRNFPVIWNEHWSFDNVVLLGDALRTAHFSIG
                     SGTRLAMEDSVALFKAFKECGADVPAALARFQETRLPPMKKILDAANSSIQWYEQMDK
                     LVATLSPVEFAYSYMTRSGRVDHAEVRRRDAKLALAYEALHPV"
     gene            7629..8537
                     /locus_tag="VGA59_04400"
     CDS             7629..8537
                     /locus_tag="VGA59_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9718950.1"
                     /translation="MELQDIDLNLLLVFDHMLKHKRVSDVAENLGLSQPAISNALARL
                     RRLLGDELFLRTPRGMEPTPFASELAEPVAFAMGALHTALNHRLLFDPATSARTFTIA
                     MTDIGEIYFTPPLMDLLSRTAPGVTISTVRNTAVNLRDEMEAGRVDLAIGLLPQLKTG
                     IFQQRLFMQRYVCIFGREHPLARKRSVSLKDFCSAEHVLVKAAGTGHGRADDVIAAQG
                     IARRVRLTVPHFVAVGHILRSTQMIATVPERLAQSIAEPFALVWRPHPVALPEVAINM
                     VWHAKFHRDPGNQWLRATLFGKFADA"
     gene            complement(8625..>9968)
                     /locus_tag="VGA59_04405"
     CDS             complement(8625..>9968)
                     /locus_tag="VGA59_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718951.1"
                     /translation="LPFSLAAARQAFRLGAYEDVLRHVDPLLAHRPAHPEALALRAEA
                     TDALGRPGTLAAYDAAADVAPEEQAHELRAKRALAQVKMSDPTGALKYLRNVHPTTVA
                     GRLAEALAYSGAAALGFGDPAEGTRRAAEVRRLALETGDEGSLVIASWAQAAAAHARG
                     ELHGSVWADLKETSHLPQLATRVFDGHLCITQRFLYGSRPYAEVIAFADALAAEAQRL
                     GAARGHAFAVTLRGEARLLSGRLDAAEQDLVAGGRMHRAIGGATGEALSLQRRAELAL
                     YRGRPDQARGLLDEALDVARQSDVGFHLLDRIYGTRIALAADPDAALGVLEEAQASVR
                     GPLETCPGCRITFAIPATIAAARAHEMDLAASHERQSDYLASVVMKLPAWYASLHEAR
                     AHIALAAGKLAAAREGFAAAAQGFHACGHTLDEARCHGLARRGSRAWQQELSNFG"
BASE COUNT         1683 a         3327 c         3278 g         1680 t
ORIGIN      
        1 agcggcatcg cgacgctcac cctcaaccgc ccggacaagc gtaacgccat cagcgacgat
       61 atgcgcaccg agttcgtcga tgccctcgag cgcatcggcg ccgacaaggc gatccgcgcg
      121 ctggtcgtga ccggcgccgg caagggcttc tgcgcgggcg gcgacatcgc cggcatggag
      181 cgccgcatga acgcgccggc cggagagatc gcgttcaatg gctggcaccg ccagcagcgt
      241 gtgcaccacg cgcaggccct gctgcacacg atgcccaagc ccgtgatcgc ggccgtcaac
      301 ggcgcggctt ccggcctggg cgcggacacc gcgctggcat gcgacttcat catcgccagc
      361 gagcgggcca gcttcacctg gtcctacatc caccgcggga tcgtcccgga tggcggcggc
      421 atgtatttcc ttccgcgccg cgtggggctg cccagggcca aggacctgat cttcaccggc
      481 cgcaaggtgg acgccgatga ggcgctggcg ctgggcatcg tcgaccgcaa gacgacggcc
      541 gaccgcctgg tggccgacgc acaggcttgg gcggcagaac tgacaaaggg ctcggccacc
      601 gcgctcgcgc tgggcaagac catcctgaac caggccttcg agctgtcggc gcaacaggtg
      661 ttcgcgcagg gcagccaggc gcagggcatc tgctacacga gcaccgaaca ccgcgagtcg
      721 gtgatggcct tcctggctaa aagcaccccc acgaagtgac acgcatggac gcgatctccc
      781 gactcctcaa gccgcgcagc gttgccgtcg tcggcgcctc cggcgatgcc tccaagaccg
      841 ccggccggcc ggtgtcgtac ctggtgaagc actcgttcgc gggtgagatc tatccggtca
      901 acccgaaggc ggaccgcatt ggcaagctgg cctgctaccc cgatgtggct tcactgccgg
      961 ccgtgccgga tgtcggcatc gtcctgctgg gcgccgagcg cgcccatctt gcggtgcgcg
     1021 acctggccgc gcgcgggtgc acggccgcca tcgtgctggc cagcggctac accgaaaccg
     1081 gtgaggaggg cgcgcggcgg cagaagcagc tgatggaagc ggccggctcc atgcgcatcc
     1141 tcggcccgaa caccatcggc ctggtgaacc tgaccgacaa catcgtcctg tccgccaccg
     1201 gcgcgctgga gatggaccat ttcccggtgg gctccatcgg cgtggtctcg caaagcggcg
     1261 gcatcctcgg ctcattgctg tcgcgcgcgg cggcgcgcgg catcggattg tcgaaactgg
     1321 tttcgaccag caacgaggtc gacctggaac tggcggattt catcgacgcg ctcgtggacg
     1381 acgccgccac caaggtgatc gcgctctaca tcgagacggt acgcaatccc gcgaagttcc
     1441 gggccgccgc cctgaaggcc gcgcgggccg gcaagcccat cgttgccttc aagatcggcc
     1501 gctcggaagc gggcgcgaag gccgccgtgt cgcacaccgg cgcgctggcg ggagccgacc
     1561 ggatgtacga cgcgctgttc cggcagaccg gcgtgatccg tgcgcagggt ttcgccgacc
     1621 tgctcgacat ccccgcggcg cttgcgaccg gccgcgtgct gcgtggcaac cgtgtcgcca
     1681 tcctcacctc cacgggcggc gccggcacgc tggtctccga cagccttggt gtctccggct
     1741 tcgacacgcc ggcgccggat gcgcagacgg cggcggcgct gcgggccctg caaaccggcg
     1801 atcacgcggc gctggatcgc aaccccatcg acgtgacgct cgccggcctg cagccggacc
     1861 tgctgcgcgg cgcgatcaag gcgctgctgg ccagtccaag ctacgacgcg ctcggcatca
     1921 tcgtcgggtc ttcgggcctg gccatgccgg aactcatggc ggatgcgatc caggattgcc
     1981 tgcccctttc ggacaagccg gtgatcgcct acgtgagccc gcatgcgccg gaggcggccg
     2041 ccctgctgac gcaacgcggc gtgccggcct tcgctgctgc ggaaagctgc agcgcggcgc
     2101 tcgcgggcat gctgcaggcg agccgctgga aggcgcctgc cgtcgaggcc gcatccgaaa
     2161 gcggcatcgc actggatgac ctgcccgcgg ggtcgctgga cgaggcgcag gcgaagcagc
     2221 tgttcgcccg cttcggcatc ccctgtcccc gcgagatggt tgtcgagacg ccggcggaag
     2281 gggagcgagc cgcacgcgaa ctgggcggcc gcgtggtgct gaagattctc tccggtgaga
     2341 tcacgcacaa gagcgacgtg ggcggagtcg cggtgaacct ggcgcccgag caggtgagtg
     2401 cacgcctgtc caccatggcc gacgaagtcg agaccaaggc aggcgtgcgt ccgcgccgat
     2461 tcctggtgca ggagatggtg accggcggaa ccgaactgat cctgggcatg cgtcgcgatc
     2521 ccctgggcac cgccatcttg ctggggatgg gcggcgtcgc ggcggaactg ttaaaggaca
     2581 cgagcatgcg cttgctgcct cctgaaggcg gcctgagccg ggacgaggcg ctttcaatgg
     2641 cccaggagct gaaaacatgg cccctgctcg atggcttccg gggccgtccc aaggccgatg
     2701 tggaggccct ggtgtcggcc attgtgggtt tctcgcgcat ggcggcgggc ctcggcgagc
     2761 ggctcgtgga agcggagatc aaccccgttt tcgtattgcc gcgaggacaa ggcgtccgag
     2821 cggccgacgg cgtcgttgtg cttcgccata agtgacgcgg gcggcgcaag ggaactgggc
     2881 gttcggccga ggtacgtcac ctgaatgcag ccagggccag cagaccaaac ccggcgaccg
     2941 cgagcagggc atgcaagacc acgatgccct tgggcagcgc cttcagttgg gcgtgatagg
     3001 ccagattgag aaatgccccg cctgccgcgg cgacgacaag caccccggct cccagccagg
     3061 ccagggtggg aaggcctgcc gtgaaggcgt catacagcac cagcgtgagg ccgctgccgg
     3121 cgagcaaccc gtgtcccatg gccagccagg acggcggccg ttcgctgccg gagaaacgga
     3181 tgaaagccat gatgaggccg cccattgcgg tcagtgcaaa tagaacaacg gcggtcttga
     3241 gcatgctggt ctcccgattc ttcgaagtgg cggctcctcc ccatcttcag cccgtgcctc
     3301 gcagcggcct gtaggctcag tgcctgaaac gtcgtcggag gcgcagccgt gatcgcttcg
     3361 gggtggcccg aaatagaatc tcgctggcta ctgcgcacgg cactttcccc ggatcaaaat
     3421 cgccgccgcg actcgtcaat gtctgactga atgggcgcga tatctgcgca aacttcaaaa
     3481 ggcctgctct tgaccaaagc agtcattgca ctcgaagctc cctaggccgg tagggagggc
     3541 gcttgcagct ttccacgaac ctggttggcc tcggccatag cagtctggaa gagccggtcg
     3601 gcaatcaccc gcgctattgc cactgaggct tcaagctctg cggggtccgg ggattcagga
     3661 tcaggcaccg ctcttcttct gcgtgccaac gccgtctcca aagttagcaa tttgtccgca
     3721 gctttttgcc acgcggggaa ggtagggaca acagggaaaa gagcagcgcc gtcacgacca
     3781 cggccgcgct ggtgctgccg cccagtttcc tcatgttttc ctcctggttg acggtgcgcg
     3841 ccaacgacca cgggtctcgc tcgcctcgct catttcgttg ggcttgtcgg cgaatcggca
     3901 agctacagcc tgcccatcag aaggagtacg accacgatga tcagcaccac cccaagaatg
     3961 ccgctcggtc cgtagcccca gctgctgctg tgcgaccaag tcggaatgac cccgattagc
     4021 atcagtacca aaacaatcag cagtatggtt cccaggctca tgacgtttct ccttgaaggt
     4081 ccagattcgc tgcatcaact atcgaacgcg gggggtgttt gtccatgatt tacgtcaatg
     4141 aagggccgtg cgccggaccc ttgatccagc gcatggaagt tccaggcgcg tgacctactt
     4201 tttgacggag tccgcggcga attgccggga ctcggccatc ggggtggccc acgcgccact
     4261 catcttttcc attgcctcga aggaaatgtc atgaataaag atcaagtcaa gggtgagatc
     4321 aaggaggtcg ccggcaaggt gcaggaaaag gccgggcagg tgattggaag cactgatcag
     4381 cagatcaagg gtctcgccaa acaggtggaa ggcaagacgc agaaggccgt cggcgatgtg
     4441 aaggaagtcg tcaaggaagc cgtgaaggat gtgacgaagg acgtcgcgcc caagtgagcg
     4501 tccgctggca ccggcatctg cggacgtcga gcgtcccgga cgccggtgtt ttctgttgtt
     4561 tgttctcgtg gcccgcgcgc acgtagatcc gcttctaatc cgcgccggtc gcaccggccc
     4621 tctcgaccga atccgcggtt ggtcaccctc tgttgacgaa tcaaccccgc gatgcccgct
     4681 ccgcctcgac cttggaagcg atcatgtcgc gcagctcgcg cttgaggatc ttgcccaccg
     4741 ggctgatcgg gaacgagggc atgacctcca gccgctcggg cagcttgaaa gaggcgatgt
     4801 gttgcgcacg cagatgggcg atcagctcgt cgaacgccag ccgggtgccc ggcttgagca
     4861 ccacgcaagc gcaggccttt tcgccgaaca ccgggtccgg catcgcgacc aggctgacct
     4921 gcttgacctg cggcaggccg aaaatcaggt tctcgatttc atcgcagctg atcttctcgc
     4981 caccgcggtt gatcatgtcc ttgcggcggc cttccgtata cacatagcgg ccgcgcttgc
     5041 ggacgatgtc gcccatgcgg tagaagccgt cggcggtaaa cgcctcggcg ttcttttccg
     5101 gcgcgttgta gtagccgttg atggtgtagg ggccccgcac caccagctcg cccggctcac
     5161 cgtcggggac ttcttcgccg gcgtcgtcga ggaccttgat ctcgtcgaag tcgcataccg
     5221 gcgcccccga gctttccagc agcaactcgt ccggatcgtc cagcctggtc atgttgatca
     5281 ggccctccgc ggtgccgtag acctcctgcg gtgtgcactc gaattccttg cgcagacgcg
     5341 cgcgcaactc gggcgccagc cgggcgccac cgttttgcac cacacgcagc gaggacaggt
     5401 cgcagcgctt gggcacctcg ctgttgagcc agtttgaaat cagcggtacc accgcggcga
     5461 tcaccgtcac cccttcgcgc tccaccgtcc ggaaaatttc ctcggtgtcg gtgctggtcg
     5521 cgatcaccag ggtgccgccg ctgtagaacg ctccgagcaa gcccggcgag gccaggttgt
     5581 agttgtggcc cagcggcagc acggccatga aaaccgtgtc gccatcgaag ccgccggctt
     5641 gcgcgcacaa gcgggcgttc aggacatagt cattgtgggt gcgcgggatc agcttggaca
     5701 tcgacgtggt gccgccggac agcagcatgg tcgagacctc gtcggctgcg gggcgcaccg
     5761 gattcagctg cgacgccagt gcctcctcgt gcatcgccgg agcgacgtgg gaatccagcg
     5821 cggtttgccc ggcgcccgcc tgcccgacga caacgacatg gcgcaggaac gggaagtcag
     5881 cggacatctc ggccgccatc ggccggtaat cgaaaccccc aaccgtgtcg gaaatgaaat
     5941 aggccgtcgc ctccgaagcg cggatgaagt gccgcacctc ggcatggcga tgcgcgcgca
     6001 aggccgtcac gggaattacg ccgatccagt tcaaggcaag ataggtcgtg acgaactccg
     6061 ggatgttggg caactgcatc acggcgcggt ccagcggctt cagcccgagc tcgatcaggc
     6121 ccagcgccag tcgcctggac tcggcgatca gctctgcata ggtgatccgg cgcgctccgt
     6181 acaccacggc caccttgtcc ggctggctgc gcgcggtgcg tgccaccatc tcggaaactg
     6241 tgagaccctc ccacaatccg gcctcacggt aacgctgcgc cgctgcttgc ggccagcctg
     6301 taaatccctg caacatggct cgctcctcct gaacgtgtgt tcgattcctc agaccgggtg
     6361 caaggcttcg taggccagcg ccagcttcgc atcgcgtcgc ctgacttccg cgtgatcgac
     6421 gcgcccgctg cgggtcatgt agctgtaggc gaactcgacc gggctcagcg tcgcgaccag
     6481 cttgtccatc tgttcgtacc actggatgct gctgttggcg gcatccagta tcttcttcat
     6541 cggtggcagc cgagtctcct ggaagcgcgc gagcgccgcc gggacatccg cgccgcattc
     6601 cttgaacgcc ttgaacaagg ctacggagtc ctccatggca agccgggtac cggaaccgat
     6661 ggagaagtgc gctgtccgca gcgcatcgcc cagcaacacg acgttatcga aactccagtg
     6721 ctcgttccag atgaccggga agttgcgcca gtaggagttg ttggaaatga tggggtgccc
     6781 ctggaggtcc ctcgcaaaca cccgctcgca gtgcttgatg gtgtcttcgg ggctcatgtc
     6841 cttgaacccg gcacgctgcc atgtcgcgtc ttccacctcg accaggaatg tgctcatgtc
     6901 gggccgatag cggtagtggt gggcacagaa aacgccggca tcggtttcgc ggaacgtcaa
     6961 cgtcaggctg tcgaatgcct tgctcgtgcc gtaccagatg aacttgttgc cgcgccactg
     7021 cgtggtagtg ccgaacttcg cctcgttctc gccgcggatc gatgagaagg ccccgttggc
     7081 ggctacgaca aggtcggcgt cgccgagttg ctccaggctc gtgacctctg tttcgaagtt
     7141 gatcttcaag cccagcttct cgacatacgc gtagagcagg gtcagcaact ccagccggcc
     7201 gatcgatgcg aagccgttgc ctgcaatcgg gatccgggtg tcgttgtgca cgatggtgat
     7261 gtcgggccag gtctccaggt gaggcgtcag gtagcgatac agcgcctcgt cgtccgctcg
     7321 aaggaactcg agcgcgcgat ccgagaacac aaccccgaat ccccaggtcg cgttgcgggc
     7381 gctgcgatcg aacagcgtga tctcgtggga cggatcatgc tgcttcatga gcgccgcgaa
     7441 gtaaaggccc gcgggcccag cgcctacgat cctgattttc atgcggccgc ctccatgaaa
     7501 ccagggtttc ctgctgtccg gcgaacagcg acgggtcgcc ttggaaagcg gatgatcggg
     7561 cagcgccctg tcatttgtca atagaatctc atgaatgatc taaatcactg ccgtgaatat
     7621 tgcaccttat ggaactgcaa gacatagacc ttaacctgct gctggtgttc gaccacatgc
     7681 tcaagcacaa gcgggtttcc gacgtggccg agaatctcgg cctctcgcag ccggcgatca
     7741 gcaacgcctt ggcccgcctg cggcggctat tgggcgacga gctgttcctg cgaacgccgc
     7801 gcggcatgga gcccactccc ttcgcgtccg aactggcgga gcccgtggcc ttcgccatgg
     7861 gggcgctcca cacggcgttg aaccaccggc tcctgttcga ccccgccacc agtgcgcgca
     7921 cattcacgat tgccatgacg gatattggcg agatctactt cacgccgccc ttgatggatc
     7981 tgctctcgcg cacggctccc ggcgtgacga tcagtacggt tcgcaacacg gccgtgaacc
     8041 tgcgcgacga gatggaggcg ggccgcgtcg acctggccat agggctgctc ccgcaattga
     8101 agaccggaat cttccagcag cgcctgttca tgcagcgcta cgtctgcatc ttcgggcggg
     8161 agcacccgct cgcgcgcaag cgcagtgtct cgctgaagga tttttgcagc gcggaacacg
     8221 tgctggtgaa ggcggcgggc accggtcacg gcagggccga cgatgtgatt gcggcgcagg
     8281 gaatcgcccg ccgggtccgg ttgacagtcc cgcacttcgt ggcggtcggc catatcctgc
     8341 ggtcgacgca gatgatcgcc acggttccgg agcgcctggc gcaaagcatt gcagaaccct
     8401 tcgcgctcgt gtggcggccc cacccggttg cgctgcccga ggttgccatc aacatggtct
     8461 ggcacgccaa attccatcgg gacccgggca accagtggct gcgcgcaacc ctgttcggca
     8521 agtttgcgga cgcttgaatg tgcggccacc gcagcgcccc gctgcgtcag gtccgcggcc
     8581 agatcaactg gaactcgatc tcctctttca ccaccaggca gcgcttaacc gaagttcgac
     8641 agttcctgct gccacgcccg cgaaccccgc cttgcaagcc cgtggcagcg ggcctcgtcc
     8701 agcgtatggc cacaggcatg gaagccttgc gccgcggcgg cgaaaccctc gcgcgcggcc
     8761 gccagcttgc ccgcggccag cgcgatgtgc gcccgcgcct cgtgcagcga ggcgtaccac
     8821 gcgggcagct tcatcaccac actggccagg tagtcggact gccgctcgtg gctggcggcc
     8881 aggtccattt cgtgcgcccg cgcggctgcg atggttgcgg ggatcgcgaa ggtgatgcgg
     8941 caaccggggc aggtctccag cgggccgcgc acgctcgctt gcgcctcttc cagcacgccc
     9001 agcgcggcat ccgggtccgc cgcgagggcg atgcgcgtgc cgtagatgcg gtccagcagg
     9061 tgaaagccga catcggactg gcgcgccacg tccagcgctt cgtccaggag gccgcgcgcc
     9121 tggtccggcc ggccgcggta caaggcgagt tcggcgcggc gctgcagcga cagcgcttcg
     9181 cccgtcgcgc cgccgatggc gcggtgcatc cgcccgccgg ccaccaggtc ctgctccgcc
     9241 gcgtcgagcc gcccgctcag cagcctggcc tcgccacgca gtgtcaccgc gaaggcgtgg
     9301 ccgcgcgccg cgcccaggcg ttgcgcctca gcggccagcg catcggcgaa ggcgatcact
     9361 tcggcgtagg gacgcgagcc gtaaaggaag cgctgcgtga tgcacaaatg accgtcgaac
     9421 acgcgcgttg ccagctgcgg caggtggctg gtctccttca ggtcggccca cacgctgccg
     9481 tgcagctcgc cgcgcgcatg cgcggccgcc gcctgcgccc acgaggcgat caccagcgag
     9541 ccttcgtcgc cggtctcgag cgcgaggcga cgaacttcgg ccgcgcggcg cgtgccttcc
     9601 gcggggtcgc cgaagcccag cgccgcggcg ccgctgtagg ccagcgcctc ggccaggcgg
     9661 cccgcgacgg tggtcggatg cacgttccgc aaatacttca gcgcgcccgt cgggtcgctc
     9721 atcttcacct gcgcgagcgc gcgcttggct cgcagctcgt gcgcctgttc ctcgggtgcg
     9781 acgtcggcgg cggcgtcgta ggcggcgagt gtgccggggc ggccgagcgc gtccgtggcc
     9841 tccgcgcgca gcgccagcgc ttccggatgc gccgggcggt gcgcgagcag cgggtcgacg
     9901 tggcgcagca cgtcctcgta ggcgccgagg cggaacgcct ggcgcgccgc ggccagtgag
     9961 aaaggcaa
//
LOCUS       DASWIY010000114         8335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2658491, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000114 DASWIY010000000
VERSION     DASWIY010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2658491"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VGA59_04410"
     CDS             <1..427
                     /locus_tag="VGA59_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX9718952.1"
                     /translation="AELFSIVSGFDAAYAAYNAHQAGMERFWTLKYLLQEGLEELVGT
                     VFKEGFVRADDLPLVLPVMGGADLPRGARVRLKLGEIDLITLDVKGTFVERLDVAPAP
                     LDALEEEAEDEPVAGPIAIAVDVNDTEETSAATIDSPAS"
     gene            430..1278
                     /locus_tag="VGA59_04415"
     CDS             430..1278
                     /locus_tag="VGA59_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX9718953.1"
                     /translation="MRSFSTLQIALGASFAVHAALLTVRIVDPEGFNRVFEDSPLEVI
                     LVNTKTHEKADKARAIAQTSLAGGGDAAKGRATSPLPPSALTELGDSAEEAHKKIEAM
                     QEQQMVLLAQIKKMLAALPPPDPKQPSSNPQAQAREDKRRQLVKMLAEIERRITEENA
                     RPKKRYISPSTREEVYAVYYDGLRRKIEDKGTHNFPEVAGKKLYGELTMVVTVNFDGK
                     VLGTEVAETSGSLALDRRARTIARNAGPFGRFNNAMRRKADQIVVVSRFKFTRDETLE
                     TRLTDR"
     gene            1275..2105
                     /gene="aroE"
                     /locus_tag="VGA59_04420"
     CDS             1275..2105
                     /gene="aroE"
                     /locus_tag="VGA59_04420"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX9718954.1"
                     /translation="MTDQYCVIGNPVEHSKSPWIHARFAQLTGQDLRYEKRLVERDAF
                     ARSVQAFRAEGGKGCNVTVPFKFEAASLTARQSDRARLAQACNTLRFDGPDVFADNTD
                     GVGLVSDITRNAGIDPAGRDVLLIGAGGAAAGVLGPLIEARPRRIVVANRTQAKAVLL
                     IERHAALAAQHGVRLEAPALHEVRDGFHVVINATASSMIGAAVPVPPSVLRPGALACD
                     LMYGSAAQGFVEWAQGHGAKARDGLGMLVEQAAEAFLIWRGVRPPSAQVLAELRATLA
                     "
     gene            2113..2856
                     /locus_tag="VGA59_04425"
     CDS             2113..2856
                     /locus_tag="VGA59_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX9718955.1"
                     /translation="MKALFRWVVLVVVAAAALQLFFLSRIALMATVDPQSTSFQRSEA
                     WRIATRKGKLRWRQRWMPYEEISSHLKRAVIASEDGSFTNHDGVDWEALEKAWQRNTQ
                     AEERAARQRPRPAQLAPRPARPPKVVGGSTITQQLAKNLLLSGERTLLRKGQEVVLAF
                     ALERMLSKERILEVYLNSVEWGAGVFGAEAAARHYYRKGAAQLSPYEAARLAVMLPRP
                     KYFEKVPNSGYLASRASTIVARMRDAELP"
     gene            2898..3509
                     /locus_tag="VGA59_04430"
     CDS             2898..3509
                     /locus_tag="VGA59_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9718956.1"
                     /translation="MLAKLMSLLLLGLVRVLTGAQARWYGCPPKAEQRIYFANHQSHA
                     DLVMIWAALPEELRSITRPIAAKDYWTKTPFREWITTAVFNAIYVDRARTGDQDPLEP
                     LIEALEHGDSVIIFPEGTRGNAEEPQPFKAGLFNLAQKFPQAVLVPAWIDNIQRVMPK
                     GEVVPVPILCSVTFGAPIRLEPQEERRDFLDRARQAVIALRDV"
     gene            3506..4492
                     /locus_tag="VGA59_04435"
     CDS             3506..4492
                     /locus_tag="VGA59_04435"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX9718957.1"
                     /translation="MNQFLRGLTPSQQVATLFIIVFGILFLVSVTAFLLTFREQADGH
                     DEAWHAELKNFRRLLRTSWFMVVVFWIAWAMGDTIATVLFALIAFFALREFITLSPTR
                     RGDHRSLVLAFFVVLPVQFWLVRTEHFNLFTVFIPVYVFLAIPVASALAGDTQRFLER
                     NAKLQWGIMVCVYGMSHVPALLLLDFRNYQGKGAFLVFFLVFVVQTCMLVQHVVTRRL
                     KRSPSAPSVSKSFNWYSWMLGMGVGSFAGGLLSFITPFKPGQAFAMAFVACVAGSMGH
                     LVMKALKRDRGVTAWGQRGISVTGANGLLDRVDALCFAAPIFFHSARWYFGA"
     gene            4512..5060
                     /gene="ruvC"
                     /locus_tag="VGA59_04440"
     CDS             4512..5060
                     /gene="ruvC"
                     /locus_tag="VGA59_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX9718958.1"
                     /translation="MRILGIDPGLQTTGFGVIDVAGPALAYVASGTITTSHLDRGNLP
                     ARLKVLFDGIGEVCERYRPGVAAVEIVFVNVNPQATLLLGQARGACVTALVARDLAVA
                     EYTALQMKLAVAGHGKAAKAQVQEMVKRLLKLPGLPGKDAADALGLAITHAHAGAAMD
                     RIAQATATARKSSGMYKGGRTY"
     gene            complement(5071..5247)
                     /locus_tag="VGA59_04445"
     CDS             complement(5071..5247)
                     /locus_tag="VGA59_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718959.1"
                     /translation="MLIFFRWAMLVLLLTAGVLFAFYAGTGQARFRRYGLFILMGTLA
                     AAFLFFGVLILEQL"
     gene            complement(5252..6175)
                     /gene="lpxC"
                     /locus_tag="VGA59_04450"
     CDS             complement(5252..6175)
                     /gene="lpxC"
                     /locus_tag="VGA59_04450"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX9718960.1"
                     /translation="MLQQRTLKSLSKAVGVGLHSGQRVELTLRPAQPDTGIVFRRVDL
                     PLPVDIPVSALAVTDTRLASTISNGGAKVHTVEHLMSACCGLGLDNLYVDITAEEVPI
                     LDGSSASFVFLLQSAGIELQKAPRRFIRVLRPIEVREGEGDNLKWARLEPYHGFKLSF
                     EIDFAHRVVDSTGQRVEFDLSSGSYARDIARARTFGFTKDVEMMRAKGLGLGAGMDNA
                     VVMDDYRVLNSDGLRYDDEFVKHKILDAMGDLYVIGKPLLAAYSAFRSGHALNNKLLR
                     ELLRQQGAYEVVTFEDERQAPAGFAQPLRAW"
     gene            complement(6294..7517)
                     /gene="ftsZ"
                     /locus_tag="VGA59_04455"
     CDS             complement(6294..7517)
                     /gene="ftsZ"
                     /locus_tag="VGA59_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX9718961.1"
                     /translation="MSIEMIEVEEFHLGTQIKVIGVGGGGGNAVEHMINRAVQGVEFI
                     CANTDAQALSRSAAHKSIQLGQSGLGAGSKPEKGREAAELAVDDIRTAINGAHMLFIT
                     AGMGGGTGTGAAPVIARIAKEMGILTVGVVTKPFDWEGGRRMTNADQGLAELEANVDS
                     LIVVLNEKLLEVLGDDITQDEAFAHANDVLKNAVGGIAEIINVPGHVNVDFEDVRTVM
                     GEPGKAMMGTAVASGPDRARIAAEQAVACPLLEGIDLSGAKGVLVLITAAKGSLKLSE
                     SKLAMNTIRAYASADAHVIYGTAYDDDLGDDVRVTVVATGLSRHGQRRTAPPLQVLRT
                     GTDNVSFNVPTINTVVGGPGAAQPAASQPDYGSMAVPSVWRTNRTQAAAKVDALSSGG
                     MDDFEIPAFLRKQAD"
     gene            complement(7750..8335)
                     /locus_tag="VGA59_04460"
                     /pseudo
     CDS             complement(7750..8335)
                     /locus_tag="VGA59_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
BASE COUNT         1353 a         2833 c         2743 g         1406 t
ORIGIN      
        1 cgcggagctg ttctccatcg tttcaggctt cgacgccgca tatgccgcgt acaacgctca
       61 tcaggcgggc atggaacgct tctggacgct caagtacctc cttcaggagg ggctggagga
      121 actcgtgggc accgtgttca aggagggctt cgtgcgcgcc gacgatcttc ccctggtgct
      181 gcccgtgatg ggcggcgccg acctgccgcg cggcgcgcgg gtgcgcctga agctcggtga
      241 gatcgacctg atcacgctgg atgtcaaagg cacgttcgtc gaacgcctgg atgtggcgcc
      301 ggcgccactc gatgcgctgg aggaggaggc ggaagacgag ccggtggccg gaccgattgc
      361 catcgccgtc gatgtcaacg acactgagga aacttccgcc gccaccatcg acagtccggc
      421 ttcgtgaacc tgcgctcgtt cagtaccctg cagatcgcgc ttggcgcctc ctttgccgtt
      481 catgcggcgc tgctgacggt gcgcatcgtc gaccccgagg gcttcaaccg cgtgttcgag
      541 gactcgccgc tggaagtgat cctggtcaac accaagacgc acgaaaaggc cgacaaggcc
      601 cgtgccatcg cacagacctc gctggcgggg ggcggcgacg ccgccaaagg ccgggccacg
      661 tccccgctgc cgccttcggc gctgaccgag ctgggcgact cggccgaaga ggcgcacaag
      721 aagatagaag ccatgcagga gcagcagatg gtgctgctgg cgcagatcaa gaaaatgctg
      781 gcggccttgc cgccgcccga tcccaagcag cccagcagca atccgcaagc gcaggcgcgc
      841 gaagacaagc gccggcagct ggtgaagatg ctggccgaga tcgagcggcg catcaccgag
      901 gaaaacgcgc ggcccaagaa gcgctatatc agcccttcca cccgtgagga ggtgtacgcg
      961 gtttactacg acggcctgcg ccgcaagatc gaggacaagg gaacccacaa cttccccgag
     1021 gtcgccggca agaagctcta tggcgaactc accatggtcg tgaccgtcaa cttcgacggc
     1081 aaggtgctgg gcacggaagt ggcggaaacc tcgggcagcc tggcgctgga ccgccgtgcc
     1141 aggaccatcg cccgcaatgc ggggccgttc ggccgcttca acaacgccat gcggcgcaag
     1201 gccgaccaga tcgtggtggt gtcgcgcttc aagttcaccc gcgacgaaac cctggaaacc
     1261 aggctgaccg accgatgacc gaccagtatt gcgtgatcgg caatccggtg gagcacagca
     1321 agtcgccgtg gatccacgcg cgtttcgcgc agctcacggg ccaggacctg cgttacgaaa
     1381 agcggctggt cgagcgcgat gcattcgcgc gcagtgtcca ggcgttccgc gccgaaggcg
     1441 gcaagggctg caacgtcacc gtccccttca agttcgaggc ggcctcgctg accgcccgcc
     1501 aatccgaccg cgcgcggctc gcccaggcct gcaacacgct gcgcttcgac gggcccgatg
     1561 tctttgccga caacaccgac ggcgtcgggc tggtgagcga catcacgcgc aacgccggaa
     1621 tcgatcccgc cggccgcgac gtcctgctga taggcgccgg cggcgccgcc gcgggagtgc
     1681 tgggacccct gatcgaggcc cggccgcggc ggatcgtcgt ggccaaccgc acgcaagcca
     1741 aggcggtgct gctgatcgag cggcatgcgg cgctggccgc gcaacacggc gtccgtctcg
     1801 aagcgcccgc gctgcacgag gtgcgcgatg ggtttcacgt ggtgatcaac gccacggcca
     1861 gcagcatgat cggcgccgcc gtcccggtcc cgcccagcgt gctgcggccc ggcgcgctgg
     1921 cctgcgacct gatgtacggc tccgccgcac agggcttcgt cgagtgggcc cagggtcacg
     1981 gcgccaaggc gcgcgacggc ctgggcatgc tggtggaaca agcggccgag gccttcctga
     2041 tctggcgcgg cgtgcggccg ccgtcggcgc aggtgctggc cgaacttcgc gcaaccctgg
     2101 cctaggcatc cggtgaaggc actctttcga tgggtggttc tggtggtcgt cgcggcggcg
     2161 gccctgcagc ttttcttcct gtctcggatc gccttgatgg ccactgtcga cccgcagtcc
     2221 accagcttcc agcgctccga agcgtggcgc attgccacgc gcaagggcaa gctgcgctgg
     2281 cgccagcggt ggatgcccta cgaggaaatt tccagccacc tcaaacgggc cgtgatcgcc
     2341 tcggaagatg gcagcttcac gaaccacgac ggcgtggact gggaggcact ggagaaggcc
     2401 tggcagcgca atacccaggc cgaggaacgg gcggccaggc aacggccgcg gccggcgcag
     2461 ttggcgcccc gccccgccag gccgcccaag gtcgtgggcg gctccaccat cacccagcaa
     2521 ctggccaaga acctgttgct atccggcgag cgcacgctgc tgcgcaaggg gcaggaggtc
     2581 gtgctggcgt tcgccctgga gcggatgctt tccaaggaaa ggatcctgga ggtctacctt
     2641 aacagcgtcg aatggggcgc gggggtattc ggtgccgagg ccgccgcccg ccactactac
     2701 cgcaagggcg ctgcgcagct ttcgccgtac gaagcggcgc gcctggcggt catgctgccg
     2761 cgtcccaagt atttcgagaa agtccccaac tccggttatc tggccagccg cgcttcgacc
     2821 atcgtcgcgc gcatgcggga tgcggagctg ccgtagccgg tgccgctcgg gtgccggtcc
     2881 ggcgcatatg atccgccatg cttgcgaaac tgatgagctt gttgctgctg gggctggtgc
     2941 gcgtgctgac cggcgcgcag gcgcgctggt acggctgccc acccaaggcc gaacagagga
     3001 tctatttcgc caaccaccag agtcatgccg acctggtgat gatctgggcc gcgctgcccg
     3061 aggaactgcg cagcatcacc cggccgatcg ccgccaagga ctactggacc aaaacgccgt
     3121 ttcgcgaatg gatcaccacc gcggtcttca acgcgatcta cgtcgaccgg gcgcgcaccg
     3181 gggatcagga tcccctggag ccattgatcg aggcgctgga gcacggcgac tcggtcatca
     3241 tttttcccga gggcacccgc ggcaacgcgg aggagcctca gccgttcaag gccggcttgt
     3301 tcaacctggc gcagaaattc ccgcaggcgg tgctggtgcc ggcctggatc gacaacatcc
     3361 agcgcgtcat gcccaagggc gaagtggtcc cggtgccgat cctgtgctcc gtgaccttcg
     3421 gcgcgccgat ccggctggag ccgcaggaag agcgccgcga cttcctcgac cgggcgcggc
     3481 aggccgtgat cgcgctgcgt gacgtatgaa ccagttcctg cgcggcctca cccccagcca
     3541 gcaggtcgcg acgctgttca tcattgtctt cggcatcctg ttcctggtca gcgtcaccgc
     3601 cttcctgctc acgttccgcg aacaggccga cggccacgac gaagcctggc acgcggaact
     3661 gaagaatttt cgaaggctgc tgcgcacttc ctggttcatg gtggtggtgt tctggatcgc
     3721 ctgggccatg ggcgacacca tcgccaccgt gctgttcgcg ttgatcgcct tcttcgcctt
     3781 gcgcgagttc atcacgctct cgccgacccg gcgcggcgac caccgcagcc tggtgctggc
     3841 tttcttcgtg gtgctgccgg tccagttctg gctggtgcgc accgaacatt tcaacctgtt
     3901 cacggtgttc atcccggtct acgtattcct ggcgattccg gtggcgagcg cgctggccgg
     3961 cgacacgcag cgcttcctgg agcgcaacgc caaattgcaa tggggcatca tggtctgcgt
     4021 gtacggcatg agccacgtgc cggcactgct gctgctggac ttccgcaact accagggcaa
     4081 gggcgccttc ctggtcttct tcctggtctt cgtggtgcag acctgcatgc tggtgcagca
     4141 cgtcgtcacc cgccgcctga agcgctcgcc cagcgcgccc agcgtgagca agagcttcaa
     4201 ctggtacagc tggatgctgg ggatgggcgt gggcagcttc gccggcggcc tgctgtcctt
     4261 catcacgccg ttcaagcccg gccaggcatt cgccatggcg ttcgtggcct gcgtggcggg
     4321 atcgatgggg cacctggtga tgaaggcctt gaaacgcgac cgtggcgtca ccgcctgggg
     4381 ccagcgcggc atctccgtga cgggcgccaa cggcctgctc gaccgggtgg acgccctgtg
     4441 cttcgcggcg cccatcttct tccactcggc gcgctggtac ttcggcgcct agggctagca
     4501 ccatgaagtc catccgcatc ctcggcatcg atcccggcct gcagaccacc ggcttcggcg
     4561 tgatcgacgt ggcgggcccg gcgcttgcct acgtcgccag cggcacgatc accaccagcc
     4621 acctggaccg gggcaacctg ccggcccggc tgaaagtgct gttcgacggc atcggcgaag
     4681 tatgtgaacg ctaccggccc ggcgtggccg ccgtcgaaat cgtcttcgtc aacgtcaatc
     4741 cgcaggccac gctcttgctg ggccaggcgc gcggcgcctg cgtcacggcg ctggtcgccc
     4801 gcgacctggc cgtggccgag tacaccgcgc tgcagatgaa gctggccgtc gcgggccatg
     4861 gcaaagccgc caaagcccag gtccaggaga tggtcaagcg actgctcaag ctgcccggcc
     4921 tgccaggcaa ggacgcggcc gatgccctgg gcctggcgat cacccatgcg cacgccggcg
     4981 cggcgatgga tcgcatcgcg caggcaaccg cgaccgcgcg caagagctcc ggcatgtaca
     5041 agggcggccg gacctactga agcgggaccg ctacagctgc tccaggatca gcactccgaa
     5101 gaacaggaac gccgctgcca gggtccccat caggatgaaa aggccgtagc gcctgaagcg
     5161 ggcctgcccc gtgccggcgt aaaacgcgaa caggacgcct gccgtgagca gcagcacgag
     5221 catcgcccag cggaaaaaaa tcagcatcgc ttcaccaggc ccgcaatggc tgggcgaagc
     5281 cggcgggcgc ctgccgttcg tcctcgaagg tgacgacctc atacgcgcct tgctggcgca
     5341 gcagctcgcg caacagtttg ttgttcagcg catgcccgga gcggaaagcg ctgtaggccg
     5401 ccagcagcgg cttgccgatg acgtaaaggt cgcccatggc gtcgaggatc ttgtgcttga
     5461 cgaactcgtc gtcgtagcga aggccgtcgc tattgagcac gcggtagtcg tccatcacga
     5521 cggcgttgtc catgccggcg cccagcccga ggcccttggc gcgcatcatc tccacgtcct
     5581 tggtgaaacc gaaggttcgc gcgcgcgcga tgtcgcgcgc gtacgagccc gagctcaggt
     5641 cgaactccac gcgctgcccg gtggaatcca ccacgcggtg cgcgaaatcg atttcgaaac
     5701 tcagcttgaa gccgtgatag ggctccaggc gcgcccactt caggttgtcg ccttcgccct
     5761 cgcggacctc gatggggcgc agcacccgga tgaagcggcg cggcgccttc tgcagctcga
     5821 tgccggcgct ttgcagcagg aacacgaaag acgccgagga cccgtcgagg atgggcactt
     5881 cctcggccgt gatgtccacg tacaggttgt ccagtccgag tccgcaacag gccgacatca
     5941 ggtgctccac cgtgtgcacc ttggcgccgc cgttggaaat ggtggacgcc aggcgcgtgt
     6001 ccgtcaccgc gagcgccgat acggggatgt ccaccggaag cggcaggtcg acgcgccgga
     6061 acacgatgcc cgtgtccggc tgggccggcc gcagcgtcag ctccacccgc tggccgctgt
     6121 gcaggccgac acccaccgct ttgctcagtg acttgagggt tcgttgctgg agcatggcct
     6181 tgattttact tgggggcagg cagacccgac aaaaagaaaa gggtgggccg cttgcaagac
     6241 ccacccaaag tacttcccct ggagaactct tatctctggc ccgcgtattc cagtcagtcg
     6301 gcctgcttgc gcaggaacgc cgggatctcg aagtcgtcca tgccgccact ggacagggcg
     6361 tcgaccttgg cggccgcctg cgtccggttg gtgcgccaca cgctcggcac ggccatgctg
     6421 ccgtagtcgg gttgcgacgc cgccggctgc gcggccccgg ggccgccgac cacggtgttg
     6481 atcgtcggca cgttgaagga cacgttgtcg gtgccggtgc gcagcacctg cagcggcggg
     6541 gccgtgcggc gctgcccgtg gcgcgacagg ccggtcgcca ccaccgtcac gcgcacgtcg
     6601 tcccccaggt cgtcgtcgta agcggtgccg tagatcacgt gcgcgtcggc cgatgcgtag
     6661 gcgcggatcg tgttcatcgc cagcttggac tccgacagct tgagcgagcc cttggccgcg
     6721 gtgatcagca ccagcacgcc cttggcgccg gacaggtcga tgccttcgag cagcgggcag
     6781 gccaccgcct gttcagcggc gatgcgcgcg cggtccgggc cggaggccac ggccgttccc
     6841 atcatcgcct tgccgggctc acccatcacg gtgcgcacgt cctcgaagtc gacgttcaca
     6901 tggccgggca cattgatgat ctcggcgatg ccgcctacgg cgttcttcag cacgtcgttg
     6961 gcgtgggcga aagcctcgtc ctgggtgatg tcgtcgccca gcacttcgag cagcttctcg
     7021 ttgagcacca cgatcagcga atcgacgttg gcctccagct ccgccaggcc ctggtcggca
     7081 ttggtcatgc gccgcccgcc ttcccagtcg aagggcttgg tgaccacacc gacggtcagg
     7141 atgcccatct ccttggcgat ccgcgcgatc accggcgcgg cgccggtgcc ggtgccgccg
     7201 cccatgccgg ccgtgatgaa cagcatgtgc gcgccgttga tggccgtgcg gatgtcgtcc
     7261 accgccagtt ccgcggcctc ccggcccttt tccggcttgc tgccggcgcc caggccgctc
     7321 tggcccagct ggatcgactt gtgcgcggcg ctgcgcgaca gcgcctgcgc gtccgtgttg
     7381 gcgcagatga actccacgcc ctgcaccgcg cggttgatca tgtgttccac cgcgttgccg
     7441 ccgccgccgc cgacgccgat caccttgatc tgggtgccga ggtggaattc ttcgacttca
     7501 atcatttcga tgctcatttg gtactcctga atctgtttgc agttgccgtt gaatagattg
     7561 ttctgtcgtc tcatgaaagt gatgtcggcg gatcggtaca tcgccatcgc tcctttctcc
     7621 cgcctttggg cgaggctcgc gcatgccagg cagccatcgt tcgggggcca ccgcggaacc
     7681 ggctttgccg ggccgctggt gacgcccccc tgggggggag gcggccgcag gccgcttcgg
     7741 ggggggtcca aagttcccca cgatgaaatc cttgaagcgg ccgaaagccg tcttcatcga
     7801 accgtttttc tgcgccacct tgaagccgcg cagccgcgcc atgcgcgcct cttcgagcag
     7861 acccatcacc gtggcggcgc gcgcctgcgc gaccatgtcc gacaacgcgc cggtgtactt
     7921 gggcacgccc cttcgcacgg gcttgaggaa gatgtcctcg cccagctcca ccatgcccgg
     7981 catgacggcg ctgccgccgg tgatgacgat gccggaggac agcaggtctt cgaaaccgct
     8041 gcggcggatc tctgtctgca ccagtgaata gagctcctcc acgcgcggtt cgatcacttc
     8101 cgccagcgtc tggcgcgaca acatgcgcgc actgcgctcg cccacacccg gcacttcgat
     8161 cggcgcatcg ttcgccagct ggcgcagcgc gcaaccgtat ttgatcttga tgtcctcggc
     8221 gtccttggtc ggcgtgcgca gagccatcgc aatgtcgttg gtgatctggt cgcccgcgat
     8281 ggggatcacc gccgtgtggc ggatcgcgcc gccggtgaag atcgccacat cggtg
//
LOCUS       DASWIY010000115         6599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1929011, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000115 DASWIY010000000
VERSION     DASWIY010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929011"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGA59_04465"
     CDS             <1..235
                     /locus_tag="VGA59_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEX9718962.1"
                     /translation="LVSFTRSGVKGELKVTREKFVLDARLGLLLGAFKERIESAIVRN
                     LDELLAHESPAHAFEKAITRKAAPKKAAARKKA"
     gene            239..499
                     /locus_tag="VGA59_04470"
     CDS             239..499
                     /locus_tag="VGA59_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9718963.1"
                     /translation="MAGAGYNLARFDLVSIRLAVACVQSGSLTAAAREAHLALAAASR
                     RIRELEGFGWTWKSRCPMPSLRPSGKVVPTSGFSSKARIPRG"
     gene            415..942
                     /locus_tag="VGA59_04475"
     CDS             415..942
                     /locus_tag="VGA59_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9718964.1"
                     /translation="MSDAVVAAVREGRADLGVFVEGPDTTGLNARPFRTDELVLVLPK
                     NHRLAGRTPLAFTDALDEDWISLNTGAAMLQKQQQAALAANKPFRLRMQVRSFDAVCH
                     LVASGLGLAMLPKAAALPIVGAMKLDWRPLADTWSRRRLLVATGTPTDAAVEALAAFL
                     LEPSQTSKPRPSRKQ"
     gene            977..2224
                     /locus_tag="VGA59_04480"
     CDS             977..2224
                     /locus_tag="VGA59_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9718965.1"
                     /translation="METSQPTAGPLAGLKILELGQLIAGPFAAKTLADFGADVVKVEP
                     PPPDPERPETGGGDPLRKWRLLKDGTSVWWQVQSRNKRSIALDLKDPEAQDIVRRLAQ
                     SADVLIENFRPGALEAWGLGPDELLALNPRLIVLRISGYGQTGPYRDRPGFGVVAEAM
                     SGLRHLTAEPGRVPVRVGVSIGDTLAALHGVIGILVALHHRHHCGRGQVIDVALYEAV
                     FNCMESLLPEYSAFGAVRGPAGSALPGIAPSNAYQCKDGGYALIAGNGDSIFKRLMGV
                     IDRSDLAADPALADNAGRVARVAEIDEAIGAWARQRTVDEVLAALDAASVPAGRIYTV
                     ADIAADPQYRARGMLDEIRMDDGSALAVPGIVPKLSATPGAHRRNAPRLGQDTDDVLR
                     ELGLTAEQIDGLKRRGIIARSGS"
     gene            2221..3156
                     /locus_tag="VGA59_04485"
     CDS             2221..3156
                     /locus_tag="VGA59_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX9718966.1"
                     /translation="MKRVYFNEVVTRDGFQMEPEFVPTDAKIALLDALSECGFAKIEV
                     TSFTSARAIPMLRDAEEVMGRIRRVPGVEYTVLVPNPRGAERALAARADELNLVMSTS
                     ETHNLANLRMPREKSLAALSEVIRLVDGRTPVNVSLSTCFGCPMEGEVPQDEVLRWAG
                     HFAALGVRGLTICDTTGMAHPVQVSRMVEALQRRFGGLQLTLHFHNTRGMGLANVLAA
                     AQGGIDRFDGSLGGLGGCPYAPGASGNISSEDAVHMLDAMGCETGIDVERLLAVARGL
                     PQVVGHEVPGQLAKAGLITHLHPAPPIPKQLKESA"
     gene            3153..3545
                     /locus_tag="VGA59_04490"
     CDS             3153..3545
                     /locus_tag="VGA59_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9718967.1"
                     /translation="MIDHLDHLVLTTSHEAQCVEFYTEVLGMSLEAFVGGTPPVERRA
                     FKFGNQKINLHVQGREFEPKAHLPVPGALDLCFMASIPLARVIGRLEQHGWPIVEGPV
                     LRTGATRKIRSIYVRDPDLNLIEISEPA"
     gene            3619..4341
                     /locus_tag="VGA59_04495"
     CDS             3619..4341
                     /locus_tag="VGA59_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9718968.1"
                     /translation="MTQRPFLAFACAHLTDERLYVAQLAALSDRYDSRVFVFRDQDSL
                     AGMAEELLANTPGRFTLIGLSLGGYVAFEVIRRQLPRLERLALLDTTALSDQPARHAG
                     RLADIAKVREGGIEALIPELPGRWLLPAHAQRPDLVALMGDMARSVGARGQINQQTAM
                     VGRPDSHRDLERLAVPTLVMCGRQDPVTPVADHQAMAARVPGARLEFIEDCGHLSAIE
                     QPEAVTRVLADWLDSTEPPENA"
     gene            complement(4350..4604)
                     /locus_tag="VGA59_04500"
     CDS             complement(4350..4604)
                     /locus_tag="VGA59_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HEX9718969.1"
                     /translation="MAELKAAFESAVAGSKNLSERPDNATLLKLYALYKQATAGDNTE
                     KKPGFGDMVGRAKWDAWNGLKGTSNDDAMQQYIDLVGSLS"
     gene            4747..6312
                     /locus_tag="VGA59_04505"
     CDS             4747..6312
                     /locus_tag="VGA59_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9718970.1"
                     /translation="MVTRIVAQQVAREATRKVVDSVVPVARRAGKTARVVRNNASLAA
                     GGMLGLAGERMTKVDTAWLRMDSPSNLMMIMGVWTLKPGIKYQDLCRRVQERLLEYDR
                     FRQRVREDAAGATWVEDAGFDIERHVVREKLPRRPRGEEQAALQQRVGELAMQPLDRE
                     HPLWQVHLVENYQGGSALIVRIHHCIADGIALISVTMSLVDGGAPPPERKRRQARAGA
                     EDWIADTLIKPFTDMTVRALGAAGEGAVRSLGLLRDPQKGLAGSLDLAKLAYQVLTDA
                     AALALMPDDSKTRLKGKPGREKRVAWCEPIPLEEVKAVGKALNCSINDVLLSCVAGSI
                     GEYLKSLGDDVAGQEIRAMIPVNLRPPLEQAHKLGNRFGLVPLVLPIGLDNPVERVYE
                     VRRRMGALKGSTQPLLAFGLLAVAGLLIKPAQEAMLSLFGKKTTAVMTNVPGPREKLK
                     FLGSTLEQSMFWVPQSGDIGLGVSILSYGGGVQFGVITDSTLCPDPQKIIDEFEPEFA
                     KLSLVTLMLPWGE"
BASE COUNT         1124 a         2123 c         2265 g         1087 t
ORIGIN      
        1 cctcgtgagc ttcacgcgct cgggcgtgaa aggagagctc aaggtcacca gggagaagtt
       61 cgtgctggac gcccgcctcg ggctgctgct gggcgcgttc aaggaacgga tcgaatccgc
      121 gatcgtcagg aacctggacg agctgctggc gcatgagtcg ccggcgcatg ctttcgagaa
      181 ggccattacc aggaaagcgg cgccgaaaaa ggcagccgcc aggaaaaagg cctgagcggt
      241 ggctggggcc ggctacaacc tggcccggtt cgacctggtt tcgatccggc tggcggtggc
      301 ctgcgtccag agcggcagcc tcacggcggc ggcgcgcgaa gcgcacctcg cactggcggc
      361 ggccagccgg cgcatccgcg agctggaagg gttcgggtgg acctggaaga gcaggtgtcc
      421 gatgccgtcg ttgcggccgt ccgggaaggt cgtgccgacc tcggggtttt cgtcgaaggc
      481 ccggatacca cggggctgaa tgcgcgaccg tttcgcaccg acgagctggt gctggttctg
      541 ccgaagaacc accgcctggc cgggcgcacg ccgctggctt tcaccgatgc cctggacgag
      601 gactggatca gcctcaacac cggcgcggcc atgctgcaaa agcagcagca ggcggcgttg
      661 gccgcgaaca agccctttcg cctgcgcatg caggtgcgca gcttcgatgc ggtctgccat
      721 ctggtcgcgt ccgggctggg cctggcgatg ttgccgaaag ccgccgcgtt gcccatcgtc
      781 ggcgcgatga aactcgattg gcgtccgctg gcagacacgt ggagccggcg ccgactgctc
      841 gttgccacag gtacgccaac cgatgcggcc gtggaggcgc tggctgcctt cctcctggag
      901 ccttcgcaaa cttcgaagcc tcgcccgtcc aggaagcaat agacgcatcg ccgggccggg
      961 cgcaaactgc cggcccatgg agacatcaca acccacggcg gggccgctgg ccggcctcaa
     1021 gatcctcgaa ttgggccagt tgatcgcggg accgttcgcg gccaagacgc tggctgattt
     1081 cggggccgac gtcgtcaagg tcgagccgcc gccgcccgac cccgagcggc cagagaccgg
     1141 cgggggcgac cccttgcgca aatggcgcct gctcaaggat ggcacctcgg tctggtggca
     1201 ggtccagtcg cgcaacaagc gctcgattgc gctggacctg aaggatcccg aagcgcagga
     1261 catcgtgcgc cggctggcgc agtccgccga cgtattgatc gagaacttcc gacccggcgc
     1321 gctggaagcc tgggggctcg gccccgatga attgctagcg ctcaacccgc gcctgatcgt
     1381 tctgcgcatc agcggctatg gccagaccgg gccctaccgc gaccggccgg gattcggtgt
     1441 ggtcgcggaa gcgatgagcg gcttgcggca tctcacggcc gagccggggc gcgtgccggt
     1501 gcgtgtgggc gtgagtattg gcgacacgct cgccgcgttg cacggcgtca tcgggatcct
     1561 ggtggcgctg catcaccggc accactgcgg acgaggccag gtcatagacg tcgccttgta
     1621 cgaggcggtc ttcaactgca tggaaagcct gttgcccgag tacagtgcat tcggcgccgt
     1681 gcgcggaccc gccggcagcg ccttgccggg aatcgcgccg agcaatgcct accagtgcaa
     1741 ggacggcggc tacgcgctga tcgcgggcaa cggtgacagc atcttcaaac gcttgatggg
     1801 cgtgatcgac cggtccgacc tcgcagccga tccggcgctg gccgacaacg cgggccgagt
     1861 agcgcgtgtg gccgagatcg acgaagccat cggggcatgg gctcgccaac gcacggtgga
     1921 cgaggtgctg gccgcactgg atgccgcttc cgtgccggcc ggccgcatct acaccgtggc
     1981 cgacattgcg gccgacccgc aataccgggc ccgcggcatg ctggacgaaa tacggatgga
     2041 tgacggcagt gcgctggcgg tgcccggcat cgttcccaag ctctccgcca cgccgggcgc
     2101 acaccggcgc aatgcgccgc ggctgggaca ggatacagac gacgtgttgc gcgaactcgg
     2161 cctgactgcc gaacagatcg acggcctgaa gcgccgcggc atcatcgcga gaagcggctc
     2221 atgaagcgcg tctatttcaa tgaagtggtc acgcgcgacg gtttccagat ggagcccgag
     2281 ttcgttccga ccgatgccaa gattgcactg ctcgatgcgt tgagcgagtg tggcttcgcc
     2341 aagatcgagg tcacctcgtt cacctctgcc cgggcgatcc ccatgctgcg ggacgccgag
     2401 gaggtgatgg gccgtatccg ccgtgtgccc ggcgtcgagt acacggtgct ggtgcccaac
     2461 ccgcgcggcg ccgaacgtgc gctggcagcg cgcgccgacg aactcaacct ggtgatgtcg
     2521 acctcggaaa cgcacaacct ggcgaacctg cgcatgccgc gcgagaaaag cctcgccgcc
     2581 ttgtcggaag tgatccggct cgtggacgga cgcacgccgg tcaacgtgtc gctatccacc
     2641 tgcttcggct gtcccatgga aggcgaagtg cctcaggacg aggtgctgcg gtgggccggg
     2701 cacttcgccg cgctcggtgt gcgcggcctg accatctgcg acacgacggg catggcgcac
     2761 ccggtccagg tcagccgcat ggtcgaggca ttgcagcggc gcttcggcgg attgcagttg
     2821 acattgcact tccacaacac acgcggcatg gggttggcga atgtgctggc ggccgcgcag
     2881 ggcggcatcg accgcttcga cggttccctc ggcgggctgg gcggctgccc ttacgccccc
     2941 ggtgccagcg gcaatatcag cagcgaagac gcggtgcata tgctcgatgc gatgggctgc
     3001 gagacgggca tcgatgtcga gcggctgctg gccgtggcgc gcggcctgcc gcaagtggtg
     3061 ggccacgaag tgccgggcca gttggccaag gcggggctca tcacgcatct gcacccggcg
     3121 ccacccatcc cgaagcagct caaggagtcc gcatgatcga ccatcttgac catctggtgc
     3181 tgaccacttc gcacgaggcg caatgcgtcg agttctacac cgaagtgctg ggcatgtcac
     3241 tcgaagcctt tgtcgggggc acacctcccg tggagcgccg ggcattcaag ttcggcaacc
     3301 agaaaatcaa ccttcatgtg caggggcgcg agttcgagcc caaggcacac ctgccggtgc
     3361 ccggtgccct ggacctgtgc ttcatggcgt cgattccgct ggcgcgggtg atcgggcggc
     3421 tggagcagca cggctggccg attgtcgaag gcccggtact gcgcaccggt gcgacccgga
     3481 agatccgctc gatctacgtg cgtgaccccg acttgaacct gatcgagatt tcagagcccg
     3541 cctgagcagg cgaatcgcgg gcgctcgaaa cgcgggcgtt tcgcgtgaga tcattgacgg
     3601 ccaaactgga gtattcgcat gacgcaacgc cccttcctcg cattcgcttg tgcgcacctg
     3661 accgacgagc gactctatgt ggcgcagctt gcggcgctca gcgaccgcta cgacagccgg
     3721 gtcttcgtgt ttcgcgacca ggactcgctg gccggcatgg ccgaggagct gctcgcgaat
     3781 acgccgggcc ggttcacgct gatcggcctg tcgctgggcg gctatgtcgc cttcgaggtc
     3841 atccgccggc aactgcctcg gctggagcga ctcgcgctgc tcgataccac ggcgctgtcg
     3901 gaccagcccg ctcgacacgc cgggcggctg gccgacatcg ccaaggtgcg cgaaggcggc
     3961 atcgaagcgc tgatcccgga gttgcccggg cgctggctct tgcccgctca tgcccagcgc
     4021 cccgatctgg ttgccctcat gggggacatg gcgcgcagtg tcggcgcgcg cgggcagatc
     4081 aaccagcaga cggcgatggt gggacgtccc gattcccatc gggacctgga aagactggcc
     4141 gtgcccacct tggtgatgtg cgggcggcaa gacccggtga cgcccgtggc cgatcaccag
     4201 gcgatggctg cacgcgtgcc gggtgcgcgg ctcgaattca tcgaggattg cgggcatctc
     4261 tcagcgatcg agcagccgga ggcagtcacc cgcgtgctgg ccgattggct ggattcgacg
     4321 gaaccgcctg agaacgcgtg aacggactgc tagctgaggg agcccaccag gtcgatgtac
     4381 tgctgcatgg catcgtcgtt cgacgtgccc ttcaacccat tccacgcgtc ccacttcgcg
     4441 cggcccacca tatcgccgaa gcccggtttc ttctcggtgt tgtcgccggc cgtggcctgc
     4501 ttgtacagcg cgtagagctt cagcagcgtg gcgttgtcgg gacgctcgga caggttcttg
     4561 gacccggcga cggcggattc gaacgcggct ttcagttcgg ccatgaatgc tccttgagat
     4621 cttggcggtg gggaagggca tctacctttt tgcggtatct gcgggtaact tcgcagtatc
     4681 ttacggccag ccgaccgccc agaatagggt ccgctgccga ataacgccac gccctccgga
     4741 gacttcatgg ttactcgaat agttgcccaa caagttgcgc gcgaagcgac gcgcaaggtc
     4801 gtcgattcgg tggtgccggt cgcccggcgc gccggcaaga cggcgcgggt ggttcgcaac
     4861 aacgcgtcgc tggcggccgg cggcatgctg ggcctggcgg gcgagcgcat gacgaaggtc
     4921 gacaccgcct ggctgcgcat ggattcgccc agcaacctga tgatgatcat gggggtgtgg
     4981 accctcaagc ccggcatcaa gtaccaggac ctttgccggc gcgtccagga gcggctgctc
     5041 gaatacgacc gcttccgcca gcgtgtgcgg gaagatgccg ccggtgcgac ctgggtggaa
     5101 gacgccgggt tcgacatcga gcggcatgtg gtgcgcgaga agctgcccag gcgtcccagg
     5161 ggcgaggaac aggcggcgct gcagcagcgc gtgggagaac tcgccatgca gccgctggat
     5221 cgcgagcacc cgctgtggca ggtccacctg gtcgagaact accagggggg cagcgcgctc
     5281 atcgtgcgca tccaccactg catcgcggac ggcatcgccc tgatctcggt cacgatgtcg
     5341 ctggtcgatg ggggcgcgcc gccgcccgag cgcaagcgca ggcaggcccg cgcgggagcc
     5401 gaggactgga tcgcggacac gctgatcaag ccgtttaccg acatgaccgt cagagccctc
     5461 ggtgcggccg gtgaaggcgc cgtccgctcc ctgggtctgc tgcgcgatcc gcagaaaggc
     5521 ctggccggat cgctcgatct ggccaagctg gcctaccagg tgctcaccga tgcggccgcc
     5581 ctggccctca tgcccgacga ctcaaagacc cgcctgaagg gaaaacccgg ccgcgagaag
     5641 cgcgtggcat ggtgcgagcc aattccgctg gaagaagtca aagccgtggg caaggcgctc
     5701 aattgctcga tcaacgacgt gcttctcagc tgcgtggccg gctccatcgg cgaatacctc
     5761 aagtcgctgg gggacgatgt cgcgggccag gagatccgcg ccatgatccc cgtcaacctg
     5821 cggccgccgc tggagcaggc ccacaagctg ggcaaccggt tcgggctggt gccgctggtg
     5881 ctgccgatcg ggctggacaa cccggtcgag cgcgtgtacg aagtgcggcg gcgcatgggc
     5941 gccctgaaag gcagcacgca gccgctgctg gccttcggct tgctcgcggt ggccggcctg
     6001 ctgatcaagc cggcgcagga ggccatgctc agcctgttcg gcaagaaaac gacggcggtg
     6061 atgaccaacg tgccgggccc gcgcgagaag ctgaaattcc tgggatcgac cctggagcag
     6121 agcatgttct gggtgccgca gtccggcgat atcggactcg gggtgtcgat cctgagctat
     6181 ggcggcggcg tgcagttcgg tgtgatcacc gacagcacgc tgtgccccga cccgcagaag
     6241 atcatcgacg agttcgagcc ggaattcgcg aagctatcgc tggtcacgtt gatgctgccg
     6301 tggggagaat agggctgtca ttgcggactt gatccgcaat ccatcgcaac gacgcaaaag
     6361 cattggccgg cggggcatgg atcccggatc aagtccggga tgacaaacca ctgtgactgt
     6421 cattgcgggc ttgacccgca atccatgaat tcagaagaca tggattccgg atcaagtccg
     6481 ggatgacagg cctgccctca atcttccttc gtccgcagcg tcaagaactc cgcctcgtac
     6541 tccttggcca gcgacagctt ctgcggctcg gtcagcacct tgccggcttc ctggaagaa
//
LOCUS       DASWIY010000116         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_438401, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000116 DASWIY010000000
VERSION     DASWIY010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438401"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VGA59_04510"
     CDS             <1..269
                     /locus_tag="VGA59_04510"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX9718971.1"
                     /translation="REQVPDLKRRGRALKAEAANLQSVFRQLKSERQRQSLSLQDIAD
                     RSGIDTAHLSRLENARFPNVTMDTLLRYADALNKQVIVALADKG"
     gene            complement(306..1796)
                     /locus_tag="VGA59_04515"
     CDS             complement(306..1796)
                     /locus_tag="VGA59_04515"
                     /inference="COORDINATES: protein motif:HMM:NF033572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /protein_id="HEX9718972.1"
                     /translation="MIMAQEQAILKAREVFNTMVAVVGGAAGQPKPLHEAERRLWDLG
                     LAMMRQMLEGYVAGFGQGDLGPTLEHKGRSWRRLDQPHTRRYVSVFGPLQIDRRVYGT
                     RETQKHEMVPLDAMLSLPEGDFSYLLQQWDQGFCVQNSYSQSRQSVQQILGIGQSVRS
                     LEHMSRAMAADADPFLEAQALPPAEQEGEVLVVTADGKGVPMCRVDGEGAPHGRLSKG
                     QKKQKKREACVGAVYSVDRFERTAEDVMDEMSRQQKQAQRPRPQHKRLRAELTRPDEQ
                     GREVRGKQRTFAWLAEQVAARNPGGEKPVVCLMDGAAALWAMAAAYLNGVTCVLDLLH
                     VMEYIWKAAHCFHPEASVQAHRFASERLKRILEGDVGRVVGGLRQMRTRAGKRLSKAK
                     RQTLETVIGYLDRNRDYMRYDAYLAAGYPIASGVVEGACRHLVADRMELSGMRWWIPG
                     AQAMLHLRAIHLNGDWDDFQRYRIEQATNRLYPDRNRVIDRWKRAA"
     gene            complement(1985..>2202)
                     /locus_tag="VGA59_04520"
     CDS             complement(1985..>2202)
                     /locus_tag="VGA59_04520"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9718973.1"
                     /translation="DYHINVFYSDEDGSYVADIPDLKYCSAFGDTPEEAMKQVLIAKE
                     LWLESARDSGKPVPEPRYRPVIYQGAG"
BASE COUNT          369 a          772 c          634 g          427 t
ORIGIN      
        1 atcgcgagca ggttccggac ctcaagcgtc gcggccgggc cctcaaagcc gaagcggcga
       61 acctgcaatc ggtcttccgc caactcaaat ccgagcgcca gcgccagagc ctgtcgcttc
      121 aggacatcgc cgatcgcagc ggcatcgaca ccgcccacct ctcacgcctc gaaaacgccc
      181 gcttccccaa cgtcaccatg gacactctgc tacgctacgc cgacgcgctg aacaaacagg
      241 tcatcgtggc gctggcggat aagggctgac ttgtgagaat ttgggtgtaa ctcgccagcc
      301 gcgagctatg cggcgcgttt ccatcggtca atcacacgat ttcggtccgg gtagagcctg
      361 ttcgtcgcct gctcgatgcg gtagcgttgg aagtcatccc aatcgccgtt gaggtggatc
      421 gcacgcaggt gcagcatcgc ctgggcgccg ggtatccacc agcgcatgcc cgacagttcc
      481 atccgatcgg cgaccaggtg gcggcatgcg ccttccacga cgccgctggc aatcggatag
      541 ccggcggcaa gatacgcgtc gtacctcatg taatctcggt tgcggtccag gtagccaatc
      601 accgtctcca aggtctggcg tttggctttg ctcagccgct tgcccgcccg ggtgcgcatc
      661 tggcgcaacc cgcccaccac gcgtccgacg tcgccctcca ggatacgctt gagccgttcg
      721 ctggcgaagc gatgcgcttg gacgcttgct tcgggatgga agcagtgggc ggccttccag
      781 atgtattcca tcacgtgcag caggtccagg acgcaggtca cgccgttcag atacgccgcc
      841 gccatcgccc acaacgccgc ggcgccgtcc atcaggcaga ccaccggttt ctcaccgccg
      901 gggtttcgcg ccgcgacttg ctcagccagc caggcgaatg tccgctgctt gccgcggacc
      961 tcccggccct gctcgtccgg ccgcgtcaac tccgcacgca gccgcttgtg ttgcggcctc
     1021 ggccgctggg cctgcttctg ctgccggctc atttcgtcca tgacgtcctc ggccgtgcgc
     1081 tcgaagcgat cgacgctgta gaccgccccc acgcacgcct cacgcttctt ctgcttcttc
     1141 tggcccttgc tcagtcggcc atggggcgcg ccctcgccat cgacgcggca catcggcaca
     1201 cccttgccgt cagcggtcac caccaggacc tcgccctcct gttcagccgg cggcaatgcc
     1261 tgcgcttcca ggaacggatc ggcgtcggcc gccatcgccc ggctcatgtg ctccaggctg
     1321 cgcaccgact ggccgatgcc caggatctgc tgcacactct ggcgcgactg gctgtaggag
     1381 ttctgcacgc agaacccctg gtcccattgc tgcaacagat aagagaagtc gccctcgggc
     1441 agactcagca tcgcgtccag cgggaccatt tcgtgcttct gcgtctcgcg cgtcccgtac
     1501 acccggcggt cgatctgcaa cgggccgaac accgacacgt accgtcgcgt gtgcggctga
     1561 tccagtcggc gccatgagcg tcctttatgc tccagcgtcg gccccagatc gccctgaccg
     1621 aacccggcga cgtagccttc gagcatctgc cgcatcatcg ccaagcccag gtcccacagc
     1681 cgacgctccg cttcgtgcag cggcttgggc tggccggcgg ccccgcccac caccgcgacc
     1741 atcgtgttga aaacctcacg cgccttgaga attgcctgct cctgtgccat gatcatgatg
     1801 aggcctccat gcctggggtg aaacgtggac ggtgactacc cacgttttat ctcaggcggc
     1861 ctctttttca agccgacatc aacgtaagtc atgtattttc aatgacctgc ctggcgcggg
     1921 cggcgaatta cacccttttc atttgaccgg cggagtcggc gcgcatgcct cgcgagaagc
     1981 cggattaacc cgcgccctgg tagatcaccg gccggtagcg aggctcggga acgggcttgc
     2041 ctgaatctcg ggcggattcc aaccaaagct ctttcgcgat gagcacttgc ttcatcgctt
     2101 cttcgggtgt gtcgccgaag gccgagcaat acttcagatc aggaatgtcc gctacgtaac
     2161 tgccgtcttc gtcgctgtag aagacattga tgtgatagtc tt
//
LOCUS       DASWIY010000117         4820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1552950, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000117 DASWIY010000000
VERSION     DASWIY010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552950"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VGA59_04525"
     CDS             <1..346
                     /locus_tag="VGA59_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEX9718974.1"
                     /translation="AGGEPASARIAMQQQSDDSFEALVTPAAGVGAWAAYWTVTEHGH
                     SSKVRAGENAGEFLQHDFVVRQYVPAGEYRSAARIILRAVASQPFHPRRVNLVVFDPK
                     SGKPLQALSLGC"
     gene            complement(374..922)
                     /locus_tag="VGA59_04530"
     CDS             complement(374..922)
                     /locus_tag="VGA59_04530"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX9718975.1"
                     /translation="MSRASGFRVFDTAIGSCGIAWDDRGQIVGTQLPQKDEDGTRACM
                     RRRFPGVPETDPLPEVQDVMRRIRALLEGHREALLDVPLDMTGVPAFHQRVYEITRAI
                     PPGRTLTYGEVAARLGEPGAARAVGRALGHNPFAPVVPCHRVLAAATGPGGFSAEGGV
                     ATKLRMLQIEKAQLGSEPGLFD"
     gene            complement(944..1903)
                     /locus_tag="VGA59_04535"
     CDS             complement(944..1903)
                     /locus_tag="VGA59_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9718976.1"
                     /translation="MPDLILHHYPSSPFSEKIRLVLGYKKLAWKSVIIPAIMPKPDVV
                     ALTGGYRKTPFLQVGADVYCDSALICDVLEHVQGEPALYPPHLKGVSRVFAQWADTTL
                     FWAAMAYNLQPKGAAYVFGRGATPSETAKAFAEDRKAMASNFVRLRPADATCAYRSYL
                     RRIAHMAEEHDFLFGTDPCVADFATYHSLWYTRAQVPVLAEILAATPAVAEWMDLMAA
                     IGHGVMDPFDAADAITVAAAAEPMPPGQNLLIDSAFQDDHGIALGSRVTITAESFGPE
                     PTEGVLLAATRTHYTLQREDPRAGMMHVHFPRVGYVLRKAEGA"
     gene            complement(1941..3584)
                     /locus_tag="VGA59_04540"
     CDS             complement(1941..3584)
                     /locus_tag="VGA59_04540"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEX9718977.1"
                     /translation="MSELTHEVFNQAEPLVDYNLFEGNRALRDALKFNAPQLDSGPLA
                     VLGAKLGSQEMQAHARLANVHTPQLRSHDRVGRRIDLVEFHPSYHALMAAATAAGLHG
                     TPWTSKAASPHVLRAASFMLFTELEPSTLCPISMTYAVTPALRRNEAVYADWGRGLAS
                     RAYDPAMKLWRDKPGLTMGMGMTEKQGGSDVRSNTSEAVPDGQDSWGQRYRVTGHKWF
                     LSAPMCDAFLVLARTPGGLSCLFLPRVLPDGSINAMRIQRLKDKLGNKANASSEVEFH
                     EATAWLVGEEDRGVALILEMGTITRMDCSLGTSGLMRQALSIALNHTAQREAFGKRLI
                     EQPLMRNVLADLALESEAATALALRVARAFDNSSDAHEAVMARVLTPIAKFWICKRGS
                     HFAQEAMECLGGNGYVEENGEGVMARVYREMPVNSIWEGSGNIMALDLLRALKKADGG
                     AALAHELAPARGGHPALDKLVAALPARVEEMATEIEARRLCQDVALAVQASLLLQTAP
                     TAVFGAFCDSRLAGNWGHTFGTLAAGTDFDAIVTRAMPR"
     gene            complement(3686..4702)
                     /locus_tag="VGA59_04545"
     CDS             complement(3686..4702)
                     /locus_tag="VGA59_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX9718978.1"
                     /translation="MPQNKQFLLDNRPQGEATAGNFKLVTGETPPLQEGQVLVRHHYL
                     SLDPYMRGRMNDAKSYAAPQPLGKVMQGGTAGEVVESRSPKYQPGDKVVGSGGWQEYS
                     VVDADQPGALRKVDTTQVPLSHYLGAVGMPGVTAWYGLAKIIEPKAGETVVVSAATGA
                     VGSAFGALAKARGCRAVGIAGGPDKCKYAVVELGFDACIDYKEHKDAMSLSKALKEAC
                     PGGIDGYFENVGGMVLDAVMLRMNAFARIALCGMIAGYDGKPVPMSYPQLLLTNRVKL
                     QGFIVSEHMEVWPEARKELGALVAAGKLRPRESVAQGIESAPEAFLGLLKGKNFGKQL
                     VKLA"
BASE COUNT          754 a         1617 c         1604 g          845 t
ORIGIN      
        1 ggccggcggc gagccggcaa gcgctcgcat cgcgatgcaa cagcagtccg atgacagttt
       61 tgaggcgctg gtgactccgg cagcgggcgt cggtgcctgg gccgcctact ggaccgtaac
      121 cgagcacggc cacagcagca aggtcagggc cggagaaaac gcgggcgaat tcctgcagca
      181 tgacttcgtg gtgcggcagt atgtgccggc gggtgagtat cgcagcgcgg ccaggatcat
      241 cttgcgcgcc gttgcatcgc agccattcca cccgcgccgg gtgaacctcg tggtgttcga
      301 tccgaagtcg ggcaagccct tgcaggcgct cagcctgggc tgttgaagcg ggcgcagggc
      361 gcggccacgt cctctagtcg aacaaccccg gctcggaccc gagctgcgcc ttttcgatct
      421 ggagcatgcg cagtttggtg gccacgcctc cttccgcgga gaagccgccg ggtccggtgg
      481 cggccgccaa cacgcgatgg caaggcacca ccggcgcgaa cgggttgtgg cccagcgccc
      541 gtcccacggc gcgcgcggca ccgggctcgc ccagccgcgc cgccacctca ccataggtca
      601 gggtgcgacc cggcgggatg gcgcgcgtga tctcgtagac gcgctggtgg aaggcgggta
      661 caccggtcat gtcgagcggg acatcgagca gcgcttcgcg atggccttcc agcagcgcgc
      721 gaatccgcct catgacgtcc tgcacttccg gcaatggatc cgtttccggc acgccaggga
      781 agcgccttcg catgcaggcg cgagtgccgt cctcatcctt ttgcggcaac tgcgtgccca
      841 cgatctgccc gcggtcgtcc caggcgatgc cgcaactgcc gatggcggtg tcgaaaacgc
      901 ggaaacccga tgcgcgtgac atcagccggc gctctttccg cgctcacgcg ccttcggctt
      961 ttcgaaggac gtatccgacg cgaggaaaat gcacgtgcat catgccggcg cgggggtctt
     1021 cgcgctgcag cgtatagtgc gtgcgcgttg cggcgagcag cacgccctcc gtcggctcgg
     1081 gcccgaaact ctctgccgtg atggtgacac ggctgcccag ggcaatgcca tggtcgtcct
     1141 ggaaggcgct gtcgatcagc aggttctgcc ccggcggcat gggctcggca gccgccgcca
     1201 ccgtgatggc atcggccgca tcgaacgggt ccatgacacc gtgcccgatg gctgccatga
     1261 ggtccatcca ttcggccact gccggcgtgg cggccaggat ctccgcgagc accggcacct
     1321 gggcgcgcgt gtaccagagc gagtggtacg tcgcgaaatc cgcgacgcaa ggatcggtgc
     1381 cgaacaggaa gtcgtgctcc tccgccatgt gggcgatgcg ccgcagatag gagcggtacg
     1441 cgcaggttgc gtccgccggc cgcagccgca cgaagttgct ggccatcgcc ttgcggtctt
     1501 ccgcgaaggc tttggccgtt tcggacggtg ttgcgccgcg gccgaacaca taggccgcgc
     1561 ccttgggctg caggttgtag gccatggcgg cccagaacag cgtggtgtcg gcccattgcg
     1621 cgaaaacccg cgacacgccc ttgaggtgcg gcgggtacaa cgcgggctcg ccctgcacgt
     1681 gctccagcac gtcgcagatg agcgcgctgt cgcaatagac gtcggcgccg acctgcagga
     1741 acggtgtctt gcggtagccc cccgtcagcg ccacgacatc gggcttgggc atgatcgccg
     1801 ggatgatcac cgacttccat gccagtttct tgtaacccag caccagccgg attttttcgg
     1861 aaaacggcga gctggggtaa tggtgcagga tcaggtcggg catgggcgtc gctccttggt
     1921 cgttgcgtcc cgcgaatcgg tcagcgcggc atcgctcgcg tcacgatggc gtcgaagtcg
     1981 gtgcccgcgg ccagggttcc gaaggtgtgg ccccagttgc ccgccaggcg cgaatcgcag
     2041 aacgcgccga acaccgccgt tggcgcggtc tgcagcagca gcgacgcctg caccgccagg
     2101 gccacgtcct ggcacagccg ccgcgcctcg atctcggtgg ccatctcctc gacgcgcgcc
     2161 ggcagtgccg cgaccagttt gtccagggcc ggatggccgc cgcgcgccgg cgccagctcg
     2221 tgcgccaggg ccgcaccgcc gtcggccttc ttcagcgccc gaagcagatc gagcgccatg
     2281 atgttgcccg agccctccca gatcgaattc acgggcatct cgcggtagac gcgggccatg
     2341 acgccctctc cgttctcttc gacgtagccg ttgccgccca ggcattccat cgcttcctgg
     2401 gcgaaatggc tgccgcgttt gcagatccag aatttcgcga tcggggtgag cacgcgcgcc
     2461 atcaccgctt catgcgcatc gctcgaattg tcgaaggcgc gggccacgcg caacgccagc
     2521 gccgtggcgg cttcggattc cagggcgagg tcggccagca cgttgcgcat cagcggctgc
     2581 tcgatcaggc gcttgccgaa ggcctcgcgc tgtgccgtgt gattcagcgc gatcgacaag
     2641 gcctggcgca tcaggccgct ggtgcccagc gagcagtcca tgcgggtgat ggtgcccatc
     2701 tccaggatca gcgcaacgcc gcggtcttcc tccccgacca gccaggcggt cgcttcgtgg
     2761 aattcgacct cggagctcgc gttggccttg ttgcccagct tgtccttcag gcgctggata
     2821 cgcatggcat tgatcgagcc gtcgggcagc acgcgcggca ggaacaggca gctcaggccg
     2881 cccggtgtgc gggccagcac gaggaaggca tcgcacatgg gcgccgacag gaaccatttg
     2941 tggcctgtca cgcgatagcg ttgcccccag ctgtcctggc cgtcgggcac ggcttccgag
     3001 gtattggacc ggacgtccga tccgccctgc ttctccgtca tgcccatgcc catggtgagg
     3061 cccggcttgt cgcgccacag tttcatggca gggtcgtagg cgcggctggc cagcccgcgg
     3121 ccccagtcgg cgtacacggc ttcattcctg cgcaacgcgg gcgtgaccgc ataagtcatc
     3181 gaaatcgggc acagcgtcga cggctccagc tcggtgaaca gcatgaacga ggcggcgcgc
     3241 aggacatgcg gcgacgcggc cttgctggtc cagggggttc cgtgcaggcc ggctgccgtc
     3301 gccgccgcca tgagggcgtg gtagctggga tggaattcga ccaggtcgat gcggcgcccg
     3361 acgcggtcgt ggctgcgcag ctgcggcgta tggacgttgg ccagcctggc atgggcctgc
     3421 atctcctggc tgcccagctt ggcgccgaga acggccagcg gcccgctgtc gagctgtggc
     3481 gcattgaatt tcaaggcatc gcgcaacgcg cggttgccct cgaacaggtt gtagtcaacc
     3541 agcggctcgg cctggttgaa cacttcgtgg gtcagctcag acatggtggc tcctcgacgc
     3601 gccaggcaat agtgtggcac tgccgggcac gttttggtgt tccaggcggt aatccgggtc
     3661 cactccggat ggccaccgcg ccaactcagg caagcttgac cagctgcttg ccgaagttct
     3721 tgcccttcag tagccccagg aaggcctcgg gcgccgactc gatgccctgc gcaaccgatt
     3781 cgcgcgggcg cagcttgccg gcggcgacga gcgcgccgag ttcctttcgc gcttccggcc
     3841 agacttccat gtgctcgctg acgataaagc cctggagctt gacccggttc gtcaggagca
     3901 actggggata ggacattggc accggcttgc cgtcgtagcc ggcgatcatg ccgcacaggg
     3961 cgatgcgagc gaaggcattc atgcgcagca tcacggcatc cagcaccatg ccgcccacgt
     4021 tctcgaagta gccgtcgatg ccgcccgggc aggcctcctt cagtgccttc gacaaggaca
     4081 tggcgtcctt gtgctccttg tagtcgatgc aggcatcgaa gccgagctcc accaccgcgt
     4141 acttgcattt atcgggtcca cccgcgatgc ctaccgcccg gcagccgcgc gccttggcga
     4201 gcgcaccgaa cgcgctcccc accgcgccgg tggccgcact gaccaccacg gtctcgcccg
     4261 ccttcggctc gatgatcttc gccaggccat accaggccgt gacgcccggc attcccaccg
     4321 cgcccaggta atgcgacagc ggcacttgcg tcgtgtcgac cttgcgcagc gcaccgggct
     4381 ggtcggcatc gaccacgctg tactcttgcc agccgccgct gccgaccacc ttgtcgcccg
     4441 gctggtattt gggcgagcgg ctttccacca cttcgcccgc cgtaccgcct tgcatgacct
     4501 tgcccagcgg ctggggtgcg gcatagctct tggcatcgtt catgcggccg cgcatatacg
     4561 ggtcaaggct gaggtagtga tgtcgaacca gcacctggcc ctcctgcaga ggcggcgttt
     4621 cgccagtgac cagcttgaag ttgccggcgg tggcctcgcc ctgggggcgg ttgtcgagaa
     4681 ggaattgctt gttttggggc atagggctct ttctagtcag ttgaggacag agcaaaattg
     4741 ctacgcaatt cttgatctgt cccgcaaggt ctcagtctgt ggaaatgatg ttcaatggga
     4801 ggacgctctt tgcgcccgtg
//
LOCUS       DASWIY010000118        15566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1930548, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000118 DASWIY010000000
VERSION     DASWIY010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 15566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15566
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1930548"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..513)
                     /locus_tag="VGA59_04550"
     CDS             complement(79..513)
                     /locus_tag="VGA59_04550"
                     /inference="COORDINATES: protein motif:HMM:NF027482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HEX9718979.1"
                     /translation="MSPLFVRFFVALAIALCASGAARAAPLSDSDTRQVQGVISAQLE
                     AFAQDDAERAFDTATPQVRQAIGSSGRFLAMVRGAYPMVYRPASVTFHKAEVDHGSVL
                     QLVEIRDEDAKSWLALFALEQQPDATWRISGCVVAENRWRST"
     gene            complement(795..1676)
                     /locus_tag="VGA59_04555"
     CDS             complement(795..1676)
                     /locus_tag="VGA59_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9718980.1"
                     /translation="MDLHEKSSGTPVRAAASVVMLRDGPGGLEVFLLKRHDLSEVLAG
                     AYVFPGGKVDPQDAAFDVQAHLDAPAAALQAALNEPELDELGAAALYVAALREAFEET
                     GVLYAQGAGARQVQLACRLLREGRAFDEVLAHMSLRLEVSRLQPWSRWITPLVGVTRK
                     RFDTRFFLASVPAGQHPRHDDHEAIDSMWLTPRAALRQYWDGRIELAPPQIMGLAHLA
                     RHASMAAALSQARLRPPPIVMPEPFELDGERLVCYPGDERHPVRERALPGPTRLHYRN
                     RRFEPAGGYDALFAEPG"
     gene            1940..2314
                     /locus_tag="VGA59_04560"
     CDS             1940..2314
                     /locus_tag="VGA59_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718981.1"
                     /translation="MSTLTKIAGVLSGISAVAVIGVAVAQSEPPNPLIPHHAIAAGQQ
                     STHITPMGETGVLAPLGELRTATITREAEVAVVAPAPAPVVEAPAPVVAAPEPPATVV
                     MAAAPEPMQPEPEPMRPRQDRN"
     gene            complement(2404..3387)
                     /locus_tag="VGA59_04565"
     CDS             complement(2404..3387)
                     /locus_tag="VGA59_04565"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HEX9718982.1"
                     /translation="MSGPKGAARFGAGICCAVLLAWLAGCAQLVPQTMGLRTAWPEGV
                     PRHVELTAVPFFPQDEYQCGPAALATVLSHGGVAITPEPLVSQVYLPSRHGSLQLEML
                     ATPRRYGRVSYQLAPRYSDLLREVAAGNPVVVLQDVGPMFTQWHYAVVNGFDYPSGTL
                     WLRSGKNERQEMPFTYFERTWVKGGHWGMVVMPPDRIPVTATEQGWMSAVLAMARVND
                     GNAATIAYAAVLKRWPDNLPAAIGLANQHHKRGALAEAVAVLRQARQRHPQSMIVMNN
                     LAQALSDQGRHAEALAQIDKAADPKSPFAADIRATRQLILQRMGQRKAGNR"
     gene            complement(3404..3754)
                     /locus_tag="VGA59_04570"
     CDS             complement(3404..3754)
                     /locus_tag="VGA59_04570"
                     /inference="COORDINATES: protein motif:HMM:NF042483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEX9718983.1"
                     /translation="MKKMTCRALVVSLLALSFQTANAGMISADQAAAANAAQTDRALV
                     MSVLDRAETTAQLQAQGIDPAMARERVAAMTDQEVQVLAQDMQTAPAGALSSAGWLAV
                     ILIAAAVWYFAFRK"
     gene            complement(4216..4467)
                     /locus_tag="VGA59_04575"
     CDS             complement(4216..4467)
                     /locus_tag="VGA59_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6587 family protein"
                     /protein_id="HEX9718984.1"
                     /translation="MVQEIIVGLIVIASAIYTFWLLAPAGVRRAGAAALAALARRFGL
                     DAQASRQLQARLAEHGGCGDCKTCNHCATLRRPPPSRTR"
     gene            complement(4469..6337)
                     /locus_tag="VGA59_04580"
     CDS             complement(4469..6337)
                     /locus_tag="VGA59_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEX9718985.1"
                     /translation="MNAMTLDLNVALLGNPNCGKTALFNLLTGSRQKVANYAGVTVER
                     KEGLLRTASGRRVRVLDLPGAYSLNPLSVDEAITRDVVTGARGDVDAPELLVCVTDAT
                     NLKLNLRLVLEARKLGLPIVVALNMSDVAHRRGIKIDVEALQRELGVPVVETVGVRHE
                     GAKALAEHLDSHARVAQPPRGAWTPPSGQDVLETQQQVRRILKAAVTEPAVDTRLDDA
                     IDRVVLHPLWGLPILATVLFLMFQAVFSWAQWPMEAVKSGMEGIGALLQSVMAEGLLR
                     SLLVDGVIAGAGGVLVFLPQILILFLFILALEDSGYLPRAAFLLDRVMGSVGLSGRSF
                     IPLLSSFACAIPGVMATRTISSWRDRIVTIMIAPLMTCSARLPVYALLIGAFIPQREV
                     AGLFNLQGLVLFALYLAGILSAMVVAWGFKHLRRNVRQAPLLMELPSYRMPNLRGLAR
                     GLLERASIFIKRVGSIILALMIVLWFISTFPAPPAGATGPAIQYSLAGMIGQWLEPLF
                     RPIGFNWQICIALIPGLAAREVAVGALGTVYALSAAGEEVAEQLAPMIGASWSVATAL
                     SLLAWYVFAPQCISTLAAVKRETNSWRYPLMMAAYLFALAYLASFITYRIAVAIGG"
     gene            complement(6341..6592)
                     /locus_tag="VGA59_04585"
     CDS             complement(6341..6592)
                     /locus_tag="VGA59_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEX9718986.1"
                     /translation="MTAVLLSSLRAGAKATVAEVSPGKTHEDRELALRLIEIGFLPGE
                     SVRVIARGQPGDEPIAVRLGHTTFALRKFEAAFVSVQAA"
     gene            complement(6750..7112)
                     /locus_tag="VGA59_04590"
     CDS             complement(6750..7112)
                     /locus_tag="VGA59_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX9718987.1"
                     /translation="MQVQVNTSNGIENKEPLERWADDYLNAELGRFKQEITCVEVQLS
                     DENKARSGASDKRCMMEARLNGHEPLAVSHHAETQDLAFRGATQKLVRKLEHTLGKLD
                     RHEHRDRGTIRKDVPITE"
     gene            7266..7934
                     /locus_tag="VGA59_04595"
     CDS             7266..7934
                     /locus_tag="VGA59_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9718988.1"
                     /translation="MAALEFIETETGPQPGATIIILHGLGADGNDFVPIARELDLAEA
                     GPVRFVFPHAPVIPVTINNGYRMRAWYDILGMDLASREDEAGLRKSMAAVEDLLEREE
                     ERGMPAHRIVMAGFSQGCAMALLTGLRHKERLAGMVGMSGYLPLARTTGSERTDANAL
                     TPIFMGHGRHDDVVDIARGTASCDALRGLGYEVEWHDYPMGHSVCMEEIEDLNAWLLK
                     VLAP"
     gene            complement(8015..8485)
                     /locus_tag="VGA59_04600"
     CDS             complement(8015..8485)
                     /locus_tag="VGA59_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9718989.1"
                     /translation="MAVTVNIDLGYEFDVKAKASEVFEVLSDVPTSVSHFPKVDKLTD
                     MGDGVFRWEMEKVGTAQVNIQTVYASKYVGNKSKGTVVWTPVEGVGNALVGGHWKIKD
                     KKKSTGIELKIEGEITLPLPGLMKMVVAPVVESEFEKLVDKYVANLIKRFGGEA"
     gene            complement(8543..8884)
                     /locus_tag="VGA59_04605"
     CDS             complement(8543..8884)
                     /locus_tag="VGA59_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718990.1"
                     /translation="MDTSTFSAAATGILANFGNTAHHLIGVFREGGERLAATLEQRWT
                     AALKESSPQLTAETRRNAARAQHAFSASYAKGLAFTADGAEVVIDTLVGAAVAAVERA
                     TAFQQARTQDA"
     gene            9101..10654
                     /gene="gshA"
                     /locus_tag="VGA59_04610"
     CDS             9101..10654
                     /gene="gshA"
                     /locus_tag="VGA59_04610"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX9718991.1"
                     /translation="MSNLHARLAALSPESLIGIRCGIEKESLRATPDGMLALTPHPQP
                     LGSALTHPHITTDYSEAQLELITGVHATPESCLEELLQIHQYTYRALGDEMLWVSSMP
                     CNLPADENIPIARYGSSNVGRAKSVYRMGLAHRYGRRMQTISGIHYNWSFPGLSSEQY
                     FALIRNFRRHAFLLLYLFGASPAVCSSFVAGRQHELRPLGEGTLFMPHGTSLRMGRLG
                     YQSDAQASLAVSYNGLDGYAASLQEALTKPYPPYEAVGIMNPGGEYNQLATGLLQIEN
                     EFYGTIRPKRVIYPGERPLHALRERGVEYVEVRLLDLNPFVPIGITSRTTRFLDTFLL
                     HCLLEASPPDTPQEIAALARNQHKTAAFGREPGLLLERGESQAPLAQWGAELMESFRP
                     IAAAQDAAHQSTDYGDALRAAQALLHEPDLLPSARVLAVMAQDFGNSFSAFTRAQSLQ
                     SREKLLALPFSGAQQARFAALSQKSVQDQKAIEAADSMPFEVYRQQYISAERLGLSRS
                     AIAPALAAV"
     gene            10983..11237
                     /locus_tag="VGA59_04615"
     CDS             10983..11237
                     /locus_tag="VGA59_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718992.1"
                     /translation="MRQKDNITVGRFLISPLTKLLENGWYASSVSIRSGSGSATHDRV
                     LRLTRLFRTKLAAVRYATAEGLQWIGHRNTSVQQAVLGAL"
     gene            11258..11509
                     /gene="infA"
                     /locus_tag="VGA59_04620"
     CDS             11258..11509
                     /gene="infA"
                     /locus_tag="VGA59_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX9718993.1"
                     /translation="MAKEELIEMKGHVDEVLPDSRFRVTLENGHQVICYTGGKMRKHH
                     IRIIAGDNVSLEMSPYDLTKGRITFRHIAGRSGPSPARR"
     gene            11643..12209
                     /locus_tag="VGA59_04625"
     CDS             11643..12209
                     /locus_tag="VGA59_04625"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEX9718994.1"
                     /translation="MTILFKQSQRVLALLVAAIFAAGPALAVQGEGRGNKHEEKAEKH
                     AQKQAGKAEKHAQKQVEKAEKRADKADKHRAKREREDVKVGAYFNDQHREAARAYYHQ
                     HYSNAKNCPPGLAKKNNGCMPPGQAKKWAVGQPIPRGVAVYTVPRAVLVQLPPPPYGY
                     RYARIGNDIVLVRTQNQLIADIIVGLLG"
     gene            12223..12882
                     /locus_tag="VGA59_04630"
     CDS             12223..12882
                     /locus_tag="VGA59_04630"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9718995.1"
                     /translation="MAGPVDPEHIARITLAHYEQRARAFWEGTRGHDVSQNTDALLRH
                     IQAAPPFELLDLGCGPGRDLKAFRDMGHRAIGLEGAASFAAMARGYSGCEVWQQNLLA
                     LDLPAARFDGIFANAVLFHVPSAQLARVLRELRASLKPGGVLFSSNPRGQGEEGWTAD
                     RYGVYHDLAGWRRYMVDAGFVELEHYYRPPGLPLEQQPWLASLWRNQAQALDSQRRSR
                     R"
     gene            complement(12840..14108)
                     /locus_tag="VGA59_04635"
     CDS             complement(12840..14108)
                     /locus_tag="VGA59_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9718996.1"
                     /translation="MARIPKRPAGLLDDVRGGVRLVIDGVAGVTGVVEAMHQRIARVS
                     PPLGVVPDAPTRGITGLVYRSIRGTTGLVGKALDAALAGVQAMLEASLRDLPRDEAMP
                     RRDAVVAAINGVVGDHLERTGNPLAIRLALRFGGASPGSAGLPAAGPRVLLLIHGLCM
                     NDSQWTRGGHDHGQALARDVGCTPVYARYNSGRHVSANARELADALEQLLRRWPVPVE
                     SMAIIGHSMGGLVARSAMHQAAQAGMHWPSKVRQLVFLGTPHHGAPLERGGNWLHRGL
                     GISPYLAPFTRLSGLRSEGITDLRHGNLLEQDWAANRFETRDTRAIVPLPQGVACYAV
                     ASTLGQKGVGARASTPPLASSARALGEAWLGDGLVPLASALGRHAKASRELHLPSSHV
                     WIGRGINHLDLLGSAAVYRRLRRWLSSAWA"
     gene            complement(14119..14259)
                     /locus_tag="VGA59_04640"
     CDS             complement(14119..14259)
                     /locus_tag="VGA59_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718997.1"
                     /translation="MAKGDQRSNKMAKKPKKDTSPPKQGTSDRPTPPVTAVIPRGKPK
                     TK"
     gene            complement(14317..15084)
                     /locus_tag="VGA59_04645"
     CDS             complement(14317..15084)
                     /locus_tag="VGA59_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEX9718998.1"
                     /translation="MSAEFSNLSLEPPFVRQERGTRSLHFTVGEIQSSMRLDRPDELQ
                     LDYTRTMMGFLLLNRQPRHIAMIGLGGGSLVKFCHGCLPTARMTVVENNPKVIALRGE
                     FEVPDDDDRLSVLEDDGAAFVRRLRGDIDVLLVDGFDHTGQPAQLCSQSFYDHCFGAL
                     AAGGVMVANLHIDHPEHDLFTARISRSFKGNAMQVLATEKANCIVFAGRRLPVTLHAL
                     RSPAWAGELEPRARRQLRGEFAHIGWNACPLGGPRST"
     gene            15253..>15566
                     /locus_tag="VGA59_04650"
     CDS             15253..>15566
                     /locus_tag="VGA59_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9718999.1"
                     /translation="MKSTAIACAIAAASLGFGSLSYAQGYNYGERDGRSHDRRGYAAQ
                     DRRGEHRDSRQAWRSGQYPQRQYDQRYRQPQYSYRHDQPRYVQRGEYSQYGQRGQWGA
                     RAP"
BASE COUNT         2688 a         5223 c         5130 g         2525 t
ORIGIN      
        1 gcgccgatcg caactgattc caaggctgtc acactcaaaa aaaccgggca ctgcccggtt
       61 tttttgagcg cgccgcggtc atgtggatcg ccagcggttc tcggcaacga cgcatccgct
      121 gatgcgccac gtggcgtcgg gctgctgctc cagcgcgaac agtgcgagcc aggacttggc
      181 gtcctcgtcc ctgatctcca ccagctgcag cacgctgccg tggtccacct ccgctttgtg
      241 gaacgtgacg gaggcgggcc ggtacaccat cgggtaggcg ccgcgcacca tcgccaggaa
      301 gcggcccgag ctgccgatgg cttgccgcac ctgtggcgtg gccgtgtcga aagcccgttc
      361 cgcgtcgtcc tgcgcgaaag cctccagctg ggccgagatg acgccctgca cctgccgggt
      421 gtcggagtcc gacagcgggg cggccctggc tgcgcccgat gcgcacaacg cgatggcgag
      481 cgccacgaaa aagcgaacga agagcgggct cattgggcgt ctccccggat gtctgggcgg
      541 acccagggta cctcggtggg cgcggcgcga agtgtaggcc gaagacgccg cgggccgtcg
      601 gacgcggcag cgttcgcaag cgggatgcag ccgggctcac tggacaatgt tcttgcattt
      661 cgcgcaagga gcgaaagccc gatacctgtc ttaatggcgc gagcccggcc ggcacgtcag
      721 agtgtggcca gcgtcctgtc gccgtcaagc aagccggctg ctcgaccgct tgtaggcagt
      781 ctccggcaag gccgctaccc gggctcggca aaaagcgcat cgtaaccacc cgcgggctcg
      841 aagcggcgat tgcggtaatg caggcgcgtc gggccgggca gcgcgcgctc gcgcaccggg
      901 tgccgctcgt cgccgggata gcagaccagg cgctcaccat cgagctcgaa cggctcaggc
      961 atgacgattg gcggcgggcg cagccgtgct tgcgacaaag cggccgccat cgaggcgtgg
     1021 cgcgccagat gggccaggcc catgatctgc ggcggcgcca gttcgatccg gccgtcccag
     1081 tactgccgca gcgccgcgcg gggcgtgagc cacatgctgt cgatggcctc gtggtcgtcg
     1141 tggcgcggat gctggcccgc cggtacgctc gccaggaaga agcgcgtatc gaagcgtttg
     1201 cgcgtgacgc ccaccagcgg cgtgatccaa cgcgaccacg gctgcagccg gctgacctcg
     1261 agccggagcg acatgtgcgc cagcacctcg tcgaacgcgc ggccttcgcg cagcaggcgg
     1321 caggcgagtt ggacctgacg cgcacccgca ccctgggcgt agagcacgcc ggtttcctcg
     1381 aaggcttcgc gcagtgccgc gacatagagc gccgcggcac caagctcatc gagttccggc
     1441 tcattgagcg ccgcttgcaa ggcggccgcc ggcgcgtcca gatgcgcctg gacgtcgaac
     1501 gccgcgtctt gcgggtcgac cttgccgcct ggaaacacat aggcgccggc caacacctcc
     1561 gacaggtcat ggcgtttgag caggaacacc tcgagcccgc cgggtccgtc gcgcagcatc
     1621 acgacgctcg cggctgcccg caccggggtg ccggaggatt tttcgtgcag gtccatgcgt
     1681 caaggctagc agagccgttg actctttgcc gatcacctgc cggacaacct gaacaaagcg
     1741 ttgcctaatt ccggcagggc tgggcttgac acgttgttcg cgaagacgcc acgtcagtgc
     1801 atgaggggca agcggggagc gctagtccga caagctttga cgcaagggcc tgacgccgca
     1861 aagccagccg ggggctactc tgcaaacagc tggcaccgaa gctggcgatt cgaccattcc
     1921 aacttgaagg agccttcgta tgagtacatt gaccaaaatc gccggcgtgc tatccggcat
     1981 ttccgccgtc gccgtgatcg gcgtagccgt cgcgcagagt gagcccccca atcctctcat
     2041 ccctcaccac gccatcgccg ctggccagca aagcacgcac atcacgccca tgggcgaaac
     2101 cggcgtgctg gcgccgctgg gcgaattgag gaccgccacg atcacgcgcg aggccgaggt
     2161 ggcggtagtt gctccggcgc cggcgccggt agtcgaggcg cccgcgcccg tggtcgccgc
     2221 tcccgaaccc cccgccacgg tagtgatggc cgcggcacct gagccgatgc agccggagcc
     2281 ggagccgatg cgcccgcgcc aggaccgcaa ctaagtgata accgcgccaa tcaaggcgcg
     2341 ggatgacggc aaggctggcc gccttgccta gtctaggagc gtaacgcgca aggctcggca
     2401 actctagcgg ttgccggcct tgcgttgtcc catgcgctgc aggatcagct gccgcgtggc
     2461 gcgtatatcg gccgcgaacg ggcttttcgg atcggcggcc ttgtcgatct gcgccagcgc
     2521 ttcggcatgg cgtccctgat ccgacaatgc ctgcgccagg ttgttcatga cgatcatcga
     2581 ttgaggatgg cgctggcgtg cttgacgcag caccgctacc gcttcggcaa gcgcgccccg
     2641 cttgtggtgc tggttggcca ggccgatggc cgcggggagg ttgtcgggcc agcgcttcaa
     2701 cactgccgca taggctatcg tcgcggcgtt tccgtcgttc acccgggcca tggccagcac
     2761 ggccgacatc cagccctgtt cagtggcagt gacgggaatc ctgtccgggg gcatcaccac
     2821 catgccccag tggccgccct tgacccatgt gcgctcgaaa taggtgaagg gcatctcctg
     2881 gcgctcgttc ttgcccgatc gcagccacaa ggttcccgac ggatagtcga atccgttgac
     2941 caccgcatag tgccattgcg tgaacatcgg gccgacgtcc tgcagcacca ccaccggatt
     3001 gcccgccgcg acctcgcgca gaaggtctga atagcgtggt gccagctgat aggagacgcg
     3061 gccatagcgc ctcggcgtgg cgagcatttc gagctggagg ctgccatgtc gcgatggcag
     3121 gtacacctgg ctcaccagcg gttcgggcgt gatggcgaca ccgccatgcg agagcaccgt
     3181 cgccaaggcc gcgggcccgc actggtactc gtcctgcgga aagaacggca ccgccgtcaa
     3241 ctcgacgtga cggggcaccc cttccggcca cgccgtgcgc aggcccatgg tctgcggcac
     3301 gagctgcgcg cagcccgcca gccacgccag cagcaccgcg cagcatatgc ccgcaccgaa
     3361 gcgagcggcg cccttcggcc cgctcatacg gccgatccga cgatcacttg cggaaggcga
     3421 aataccagac cgccgcggcg atcaggatga cggccagcca accggcggag ctcagggcgc
     3481 ctgccggcgc ggtttgcatg tcctgggcca ggacttgcac ttcctggtcg gtcatggcgg
     3541 ccacgcgttc gcgggccatg gccggatcga tgccctgcgc ctggagctgg gcggtcgtct
     3601 cggcgcgatc aaggacgctc atcaccaaag cgcggtcggt ttgcgcggcg tttgcggcgg
     3661 ctgcctggtc ggcactgatc attccggcgt tggctgtctg gaacgacagg gccagcaggg
     3721 acacgaccag cgcacggcat gtcattttct tcatcgttga tctcatcttc aggctccttg
     3781 ggatgtgttg aggtgagcgc cggcggcaag acccaccatg gcgccggatt aatatacgcg
     3841 cgtgcgcgca gcttgtgtgc tgtttgttcg cgcgcgggcc cgcgtgcctc ggggtggtca
     3901 caaatatgta caaaaaaagg tgcaccccgc cgaaggcagg agaactccta cggaggccgg
     3961 gacaggctgg gccgtaacgg ctttacccaa gtgggtgcgt gagcaacaaa gacgggtcgg
     4021 ccgcgacccc gtgcaaaatg cgttgatgca gcaaccactc tcagcagttc cgggggaggc
     4081 gtcctgcctg tgacacgggt cgacgggcga gccctgggca atggctcacc cggaccggtg
     4141 acccggcagc tgctggaaac ctactgggcc tggcatcgcg atccgcagta cagcacaccg
     4201 gtgcggtacg aaacactatc gggtgcgcga cggcggcggc cggcgcagcg tggcgcagtg
     4261 attgcaggtc ttgcaatcgc cgcagccgcc gtgttcggcc agcctcgcct gcagctgccg
     4321 gctcgcctgc gcgtccaggc caaagcggcg ggccagcgcc gcgagagcgg ccgcaccggc
     4381 acgccggacg ccggcgggcg ccagcagcca gaaggtgtag atcgccgatg cgatgacgat
     4441 cagcccgacg atgatttcct ggaccatttc agcctccgat tgcgaccgcg atgcggtacg
     4501 tgatgaacga tgcgagatag gccagcgcga acaggtaagc cgccatcatg agcgggtagc
     4561 gccacgagtt cgtctcgcgc ttgacggccg cgagcgtcga gatgcattgc ggcgcgaata
     4621 cataccaggc gagcagcgaa agcgcggtgg ccaccgacca gctcgcaccg atcatgggcg
     4681 ccagttgctc ggccacttct tcgccggcgg ccgagagcgc atagacggtg cccagcgcgc
     4741 cgaccgccac ttcgcgtgcg gccagtcccg gaatcagcgc gatgcagatc tgccagttga
     4801 atccgatcgg ccggaacagc ggttcgagcc actggccgat catgccggcc aggctgtatt
     4861 ggatcgcggg cccggtcgcg ccggccggcg gcgccggaaa agtggagatg aaccacagca
     4921 cgatcatcag tgcgaggatg atgctgccca cgcgcttgat gaagatgctg gcgcgctcca
     4981 gcaaccctcg cgccaggccg cgcaaattcg gcatgcggta gcttggcaac tccatcagca
     5041 gcggcgcctg gcgcacgttg cggcgcaggt gcttgaagcc ccacgcgacc accatcgccg
     5101 agaggatccc cgccaggtac agcgcgaaaa gcaccaggcc ctgcaggttg aacagtcccg
     5161 ccacctcacg ctggggaatg aaggctccga tcagcagggc gtagaccggc aatctcgcgg
     5221 agcaggtcat caacggcgcg atcatgatgg tgacgatgcg gtcgcgccag ctgctgatcg
     5281 tgcgagtggc catcaccccg ggaatggcgc atgcgaagct cgacagcaac gggatgaacg
     5341 agcggccgga cagccccacc gaacccatga cccggtcgag caggaaggcc gcgcgtggaa
     5401 ggtagcccga gtcttcgagc gcgaggatga acaggaacag gatcaggatc tgcggcagga
     5461 agaccagcac gccccccgcg ccggcgatca cgccgtcgac cagcaggctg cgcagcagcc
     5521 cttcggccat gacggattgc agcagggcgc ctatgccctc catgccggat ttcaccgctt
     5581 ccatcggcca ttgcgcccag ctgaagacgg cctggaacat caggaacagc accgtggcga
     5641 gaatgggcag cccccacagc ggatgcagca ccacgcgatc gatggcgtcg tccagccgcg
     5701 tgtccacagc cggttcagtc acggcggcct tcaggatgcg gcgcacctgc tgctgcgttt
     5761 ccagtacgtc ctgcccactg ggcggggtcc acgcaccgcg gggcggctgg gcgacacgtg
     5821 cgtgggagtc caggtgctcg gccagggctt tggcgccctc gtgacgcaca ccaacggtct
     5881 ccaccacagg cacaccgagc tcgcgctgca gcgcctcgac gtcgatcttg atgccgcgcc
     5941 ggtgcgccac gtcgctcatg ttgagcgcga cgacaatcgg caacccgagc ttgcgcgctt
     6001 ccagcacgag tcgcaggttg agcttgaggt tggtcgcgtc ggtgacgcag accagcagct
     6061 cgggagcgtc gacatcgccg cgtgccccgg tgacgacgtc tctggtgatc gcctcgtcca
     6121 cgctcagcgg gttgaggctg taggcgcctg ggaggtcgag cacgcgaaca cgccggccgg
     6181 aggccgtgcg cagcaggcct tccttgcgtt cgacggtcac gccggcgtag ttggccacct
     6241 tctggcggct gccggtcagc aggttgaaaa gggcggtctt gccgcagttg ggattgccga
     6301 ggagggcgac attcaggtcg agcgtcatgg cgttcatggc tcaggccgcc tggacggaca
     6361 cgaaggccgc ctcgaatttc ctcagcgcga aagtcgtgtg gccaagccgc acggcgatcg
     6421 gttcgtcgcc cggctggccg cgtgcgatta cgcgcacact ctcgcccggc aggaagccga
     6481 tttcgatcag gcgcagggcc agttcacggt cctcatgcgt cttgccgggc gacacctcgg
     6541 caacggtggc cttggccccg gcacgcaaac tgctcagcag aacggctgtc attctcaccc
     6601 cccgaagatc gcgcccaaca gtggacaacg gaaatgagaa taattctaaa ttagatttag
     6661 aagtatgacg ccgatcaaac gggagcgatt tagggcgtgc ctctcagaag attcgcacat
     6721 ccgtgcgatt ttatgaggtc gtaccgggtt tattccgtaa tggggacatc cttgcggatc
     6781 gtgccgcggt cgcggtgctc gtgccgatcg agcttgccca gggtgtgctc gagcttgcgg
     6841 accagcttct gggtggcgcc gcggaaagcc aggtcctggg tttcggcgtg gtggctcacc
     6901 gccagcggtt cgtgtccgtt gagtcgggcc tccatcatgc agcgcttgtc ggatgcgccg
     6961 cttctcgctt tgttttcatc gctgagctgc acttcgacac aggtgatctc ctgcttgaag
     7021 cggccgagct cggcgttcag gtagtcatcc gcccaacgct ccagtggttc cttgttctcg
     7081 atgccattgc tggtgtttac ttgcacttgc ataagccgag gctcctttcg ttcctcaaga
     7141 accagtgtgg gggcgacagg cgcaaagtcc tgtaggaaat tgcgccgcac cgtgtcggcc
     7201 gttgccggct ggacctgtgc cggcaccggc cgagcctgta tgctcgccgt tccccggaga
     7261 aatctatggc tgcgcttgaa ttcatcgaaa ccgaaaccgg cccccaaccg ggcgcgacca
     7321 tcatcatcct gcacggcttg ggcgccgatg gcaacgactt cgtccctatc gcccgggagc
     7381 tcgacctggc cgaggccggg ccggtccggt tcgtctttcc gcatgcgccg gtcatccccg
     7441 tcaccatcaa taacggctac cggatgcgtg cctggtacga catcctgggc atggacctgg
     7501 cgtcacgcga ggacgaagcg ggactgcgca agtcgatggc cgcggtcgag gatctgctgg
     7561 agcgcgagga ggagcgcggc atgccggccc atcgcatcgt gatggccggc ttctcgcaag
     7621 gctgcgccat ggccttgctg accggcctgc ggcacaagga gcgcctggcc gggatggtcg
     7681 ggatgtcggg ctatctgccg ctggcgcgga ccaccggcag cgaacgcacc gacgccaacg
     7741 ccctgacgcc gatcttcatg ggccatggcc ggcacgacga tgtggtggac atcgcacgcg
     7801 gcacagcctc atgcgacgcg ctgcgggggc tgggctatga ggtcgagtgg cacgactacc
     7861 cgatggggca ctcggtgtgc atggaagaga tcgaggacct caacgcctgg ttgctcaaag
     7921 tgctggcgcc ttgagcgatc cattccaggg ccaccgcgga accggctttg ccgggccgct
     7981 ggagggcctt gcaggccgct tcgtcggggg tgggctatgc ttcgccgccg aaccgcttga
     8041 tcaggttcgc gacgtacttg tcgaccagtt tttcgaactc gctttccacc acgggcgcaa
     8101 ccaccatctt catcagtccg ggcaggggca gggtgatttc accctcgatc ttgagctcga
     8161 tgccggtcga tttcttcttg tccttgatct tccagtgccc gccgaccagg gcattgccga
     8221 caccttcgac cggcgtccac accacggtgc ccttgctctt gttgccgacg tacttgctgg
     8281 cgtacaccgt ctggatgttc acctgggcgg tgcccacctt ttccatctcc cacctgaaga
     8341 caccgtcgcc catgtccgtg agcttgtcga ccttgggaaa gtgcgatacc gacgtgggca
     8401 cgtcggacaa gacctcgaac acctcggagg ccttggcctt gacgtcgaat tcatagccca
     8461 ggtcgatgtt gacggtcacg gccatgggag tctcctggat ttgttgttga atcgggtgga
     8521 cattctgcca cccgggtggc gttcaggcgt cttgcgtgcg cgcctgctgg aaggcggtgg
     8581 cgcgttccac ggccgccacg gccgcgccca ccagcgtgtc gatcaccact tcggcgccgt
     8641 cggcggtgaa ggccaggccc ttggcgtagg acgcgctgaa cgcatgctgc gcacgtgccg
     8701 cgttcctgcg cgtttcggcg gtgagctgcg ggctggattc cttgagcgct gccgtccagc
     8761 gctgctccag cgtcgcggcc aggcgctcgc cgccctcgcg gaaaacaccg atcaggtgat
     8821 gggccgtgtt gccgaagttg gcgaggatgc cggtcgcggc ggcggaaaaa gtgctggtgt
     8881 ccatggagat gctcctgttg cggattgatg agccgatttt ccctgcgcgc gttagtggcg
     8941 gcgacctaat ggggcggctt ttgctagagt ctgcggtcta gggcctgtgc acaggcccta
     9001 tccgcggggc ggaatcggcc cttgccccgg cccggtgcac gaaatccgaa gccgtgctaa
     9061 cttccactcc attccacaag acgaccggcc cttttcgaac atgagcaacc tgcacgcgcg
     9121 cctggccgcc ctttcgcccg agagcctgat aggcatacgc tgcggcatcg aaaaggaaag
     9181 cctgcgcgcc acgcccgatg ggatgcttgc gttgacaccg cacccgcagc ccctcggctc
     9241 ggcattgacc catccgcaca tcaccaccga ttacagcgag gcgcagctgg aattgatcac
     9301 cggcgtgcac gccacgcccg aaagctgcct ggaagagctg ctgcagatcc accagtacac
     9361 ctaccgggcg ctcggcgacg agatgctgtg ggtgtccagc atgccgtgca acctgccggc
     9421 ggatgaaaac attcccatcg cgcgctacgg ctcctccaac gtgggacgcg ccaagagcgt
     9481 ctaccgcatg ggcctggccc accgctacgg gcggcgcatg cagacgatct cgggcatcca
     9541 ttacaactgg tccttccccg ggctttcgag cgagcagtat ttcgcgctga tccgcaactt
     9601 ccgccgccat gccttcctct tgctctacct gttcggcgcg tcgccggccg tgtgttccag
     9661 cttcgtcgcc ggccgccagc acgagttgcg gccgctgggc gagggcacgc tgttcatgcc
     9721 gcacggcacc tcgctgcgca tggggcggct gggctaccag agcgacgcgc aggccagcct
     9781 ggccgtgagc tacaacggcc tggacggcta cgcggcgtcg ctgcaggagg cgctcaccaa
     9841 accgtatccg ccctatgaag cggtgggcat catgaatccg ggcggcgaat acaaccagct
     9901 ggccaccggc ctgttgcaga tcgagaacga gttctacggc acgatcaggc caaagcgggt
     9961 gatctacccc ggcgagcggc cgctgcacgc gctgcgcgag cgcggtgtgg agtatgtcga
    10021 ggtgcggctg ctcgacctca atcccttcgt gcccatcggc atcacgtccc ggacgactcg
    10081 ctttctggac accttcctgt tgcattgcct gctggaagcc agcccgcccg acacgccgca
    10141 ggaaatcgcc gcgctggcgc gcaaccagca caagacggca gccttcggcc gcgaaccggg
    10201 cctgttgctc gaacggggcg aaagccaggc ccctcttgcg cagtggggag ccgaactgat
    10261 ggaatcgttc cggccgatcg ccgcggcgca ggacgcggcg caccagagca ccgattacgg
    10321 cgatgcgctg cgtgccgccc aggctctgct gcacgaaccc gacctgttgc cctcggcccg
    10381 tgtcctggcg gtgatggcac aggacttcgg caattcgttc tccgctttca cccgggcgca
    10441 atcgctgcaa tcgagggaaa aactgctggc cctgccgttc agcggcgccc agcaggccag
    10501 gttcgcggcg ctcagccaga agtcggtgca ggaccagaaa gcgatcgaag cggccgactc
    10561 catgccgttc gaggtctatc gccagcagta catatcggcg gaacggctgg gcctgtccag
    10621 gagcgcgatt gcgcccgcgc ttgcggcagt gtgagcggcg ccggccccgc gtgaggcttt
    10681 cccccgtctt cgcctgcgcg aaagaggtga tgcgggtaca ataggcactt cgattgcgca
    10741 ggcgattgaa cgcgccaata aaaccctatt ccaggcgcgc cagaccccca ctgcgcacgc
    10801 gtctcccggg gcatcccaag gccccttgca ccggccggcc aagccgggct ttcctccaga
    10861 cgcagtcccg gccctccgag agaggccatg ccagcgggtt cgatatgcgc cggcttgcca
    10921 aggcaagcct tcgaacagta tcgcggccgc aatgttgcgc gccgcaggac catcaaaaaa
    10981 caatgcgaca aaaagacaac atcaccgtcg gccgattcct gatatcgccg ctgacgaaac
    11041 tgctcgagaa cggctggtac gccagttcgg tctcgatccg ctcgggctcg ggcagcgcca
    11101 cgcacgaccg cgtgctgcgc ctgacccggc tattccggac caagctcgcg gcggtgcgct
    11161 atgccaccgc cgaaggcctg caatggatcg gccaccgcaa caccagcgtt cagcaagccg
    11221 tcctcggcgc gctctaaacc ataccggaga gaacctcatg gccaaggaag aactgattga
    11281 aatgaagggg catgtggacg aagtgctgcc cgattcgcgc tttcgcgtga cgctcgagaa
    11341 cggccaccag gtgatctgct acaccggcgg caagatgcgc aagcaccaca tccgcatcat
    11401 cgccggggac aacgtctcgc tggaaatgtc gccctacgac ctgaccaagg gccgcatcac
    11461 gttccgtcat atcgcgggcc gcagcggtcc gtcgccggcc cggcgctgag ccggcgggag
    11521 cgccgcccac agcggccggc ggtcgattcg gcgccctttc ggggcgccgt cctacgtggc
    11581 gtggtcaacg ccgagcagaa agtggcgtta caagtgtagt cacacatcgg cgggagccca
    11641 caatgaccat tctgttcaaa caatctcaac gcgtgcttgc cttgctggtt gcggccattt
    11701 tcgcagcggg tccggcgctc gcagtccaag gcgaaggccg cggcaacaag catgaggaga
    11761 aggccgaaaa gcatgcccag aagcaggccg gcaaggcgga aaagcatgcc cagaagcagg
    11821 tggaaaaggc ggagaagcgc gccgacaagg ccgacaagca ccgcgccaaa cgcgaacgag
    11881 aggacgtcaa ggtcggggcg tatttcaacg accagcaccg tgaagcggcg cgtgcctact
    11941 accaccagca ttacagcaac gcgaagaact gccccccggg gctcgcgaaa aagaacaacg
    12001 gctgcatgcc ccccggccag gcgaagaagt gggccgttgg ccagccgatc ccgcgcggcg
    12061 tcgcggtcta taccgtaccg cgagcggtgc tggtccaact gccgcccccg ccttacggtt
    12121 accgctacgc gcgcatcgga aacgacatcg tgctggtgcg cacgcagaac cagctgatcg
    12181 ccgacatcat cgtgggtctg ctgggctgaa cgctggcata gcgtggcggg acccgtcgac
    12241 cccgaacaca tcgcgcggat cacgctggcc cactacgagc agcgcgcgcg ggctttctgg
    12301 gaaggcacgc gcggccacga cgtcagccag aacaccgatg ccctgctgcg gcacatccag
    12361 gccgcaccac cgttcgaatt gctggacctg ggctgcggtc ccggccgcga cctcaaggca
    12421 tttcgcgaca tgggccaccg cgccatcggc ctggagggcg ccgcctcctt cgcggccatg
    12481 gcgcgcggat acagcggctg cgaagtgtgg cagcagaacc tgttggcgct cgacctgccg
    12541 gcggcgcgct tcgacggcat cttcgccaat gccgtgctgt tccatgtccc gagcgcgcag
    12601 ctggctcgcg tgttgcggga gctgcgcgcc tcgctgaagc ccggcggcgt gctattcagc
    12661 tccaaccccc gcggccaagg cgaggaaggc tggaccgcgg accgctatgg cgtctaccac
    12721 gacctcgccg gctggcgtcg ctacatggtg gacgccggct tcgtggaact cgaacactat
    12781 taccggcccc cgggcctgcc actcgagcag cagccctggc tggcgagcct gtggcgcaat
    12841 caggcccagg cacttgacag ccagcgccgc agccgccgat agaccgccgc gctgccgagc
    12901 aggtcgagat gattgatgcc gcggccgatc cagacatgcg aagacggcag gtgcagctcg
    12961 cggctggctt tggcatgccg gccgagggcg ctggccagcg gcaccagtcc atcgcccagc
    13021 cacgcctcgc ctagggctcg cgcgctggac gcgaggggcg gcgtactcgc acgggcgccc
    13081 acgccctttt gtcccagagt gctcgcgacg gcatagcagg cgacgccctg cggcagcggc
    13141 acgatggcgc gtgtgtcgcg cgtctcgaaa cggttggccg cccagtcctg ctcgagcagg
    13201 ttgccgtggc gcaggtcggt gatgccttca ctgcgcagcc cgctgagccg cgtgaacggc
    13261 gccaggtagg ggctgatgcc caggccgcga tgcagccagt tccccccgcg ttccagcggc
    13321 gcgccgtggt ggggcgtccc caggaacacc agctgccgca ccttggacgg ccagtgcatc
    13381 ccggcctgcg cggcctggtg catggcgctg cgcgccacca gcccgcccat gctgtggccg
    13441 atgatggcca tgctctccac cgggaccggc cagcgccgca gcagttgttc gagcgcgtcg
    13501 gccagctcac gcgcgttggc cgaaacgtgg cggccgctgt tgtagcgcgc atagaccggc
    13561 gtgcaaccca cgtcacgggc cagcgcctgg ccatggtcat ggccgccgcg tgtccattgc
    13621 gaatcgttca tgcacagccc atggatcagc agcaatacgc gcgggccggc cgccggcaac
    13681 ccggctgagc ccggcgacgc accgccaaag cgcaacgcca gccggatggc cagcgggttg
    13741 ccggtgcgtt ccaggtggtc gccgacgacg ccgttgatgg ccgcgaccac cgcatcgcgg
    13801 cgcggcattg cttcgtcccg cggcaagtcg cgcagcgagg cttccagcat cgcctggacg
    13861 ccggccaagg ctgcatcgag ggccttgccg accaggccgg tggtgcctcg gatgctgcgg
    13921 tacaccaggc cggtgatgcc ccgggtcggc gcgtcgggga ccacgcccag cggcggcgaa
    13981 acgcgcgcta tgcgctggtg catcgcctcg acgacgccgg tgacgccggc gaccccatcg
    14041 atcaccaggc gcacgccgcc acgcacatcg tccagcagcc cggcgggacg cttgggtatc
    14101 ctggccaatg ccggccgctt acttggtctt cggcttgccg cgcgggatca ccgcggtgac
    14161 gggcggcgtc gggcggtccg acgtgccttg cttgggcggc gaagtgtcct ttttcggttt
    14221 cttcgccatc ttgttgctgc gctggtcgcc tttggccatg agagcctcca tgtcggtgtg
    14281 gaagggattg atgggtagag atggtatacg cggaggctag gttgaccgcg gcccgcccag
    14341 cgggcaggcg ttccagccga tatgggcgaa ctcgcccctg agttgccgcc gggcccgcgg
    14401 ctccaactcg cctgcccacg ccgggctgcg cagggcgtgc agcgtgactg gcaggcggcg
    14461 gccggcgaag acgatgcagt tggccttttc cgtcgcaagc acctgcatgg cattgccttt
    14521 gaagctgcgc gagatccgcg ccgtgaaaag gtcgtgctcg ggatggtcga tgtgaagatt
    14581 cgccaccatc acgccaccgg cggcgagcgc gccaaaacag tggtcgtaaa acgactgcga
    14641 acacagctgc gccggctggc ccgtatggtc gaagccgtcg accagcagca cgtcgatgtc
    14701 accgcggagc ctgcgcacga acgcggcgcc gtcatcctcc agcaccgaca gccggtcgtc
    14761 atcgtcgggc acctcgaatt caccgcgcag ggcgatcact ttggggttgt tctccaccac
    14821 cgtcatccgc gccgtcggca ggcagccgtg gcagaacttc acgagcgagc cgccgcccag
    14881 cccgatcatg gcgatgtggc gcggctgccg gttcaagagc agaaaaccca tcatcgtgcg
    14941 ggtgtagtcc agctgcagtt cgtccggccg gtccaggcgc atgctgctct ggatctcgcc
    15001 gacggtgaaa tgcaatgacc tcgtgccccg ttcctgccgg acgaagggag gctcgaggct
    15061 caggttggag aattcagcgg acatgcccga ttgtgatccg gccacccgga cgcggcgcag
    15121 gtgagaaagc gcttctgccg gtgcggccgg cacaggcggc aaccctactt tgcaaagcgc
    15181 ccacccgcgc tttgcaagcc gtacacgtgg gaggcggatc atcggcttac caccacctgt
    15241 agggaagcca tcatgaaatc aaccgccatc gcctgcgcca tcgccgccgc atcgctgggc
    15301 ttcggctcgc tgtcgtatgc gcaaggttac aactatggcg agcgcgatgg gcgcagccac
    15361 gatcgccggg gctacgccgc ccaggaccgg cgcggcgaac accgcgattc gcgccaagcc
    15421 tggcgaagcg gccagtaccc gcagcgccag tacgaccaac gctaccggca gccgcagtac
    15481 agctatcgtc atgaccaacc acggtacgtt cagcgtggcg aatacagcca gtatggccaa
    15541 cgcggccaat ggggcgcgcg cgcgcc
//
LOCUS       DASWIY010000119         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_66485, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000119 DASWIY010000000
VERSION     DASWIY010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66485"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VGA59_04655"
     CDS             <1..1054
                     /locus_tag="VGA59_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX9719000.1"
                     /translation="NTARLQSMADARRKRGEERQVLQAGFLALDPRNGHVKAWVGSRD
                     FAQEQFDHVSQARRQPGSTFKPFVYGAAFVEGFHPSSTFIDEIVDYRSPGGEVWTPSD
                     GTPPSGQPMSLRDGLVYSKNTITAQLMRQVGPVRVARLAQAMGVRQSKLDLVPSLALG
                     TSPVTLREMVAAYGTIANSGTYTEPLLVIRVEDRQGRTLEGFVSRQPEQAMPRASALT
                     LVNVMRGVVDEGTGVAIRTRYGIQADVAGKTGTTQDNSDGWFILMHPQLVAGAWVGFN
                     DNRMVMGGQWGQGARSALPIVGDYFQQSLRWRWIDAQAEFAIPRPRPAPPPQRPQDAL
                     SDIVNDLLGRILRRLQ"
     gene            complement(1409..>1644)
                     /locus_tag="VGA59_04660"
     CDS             complement(1409..>1644)
                     /locus_tag="VGA59_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly(3-hydroxyalkanoate) depolymerase"
                     /protein_id="HEX9719001.1"
                     /translation="TGHSETDKGVSAGGRGYSRSVHRGPGGEVVAEHWVVHGAQHAWS
                     GGSAKGSYTDPRGPDATGEMVRFFMEHPHRARH"
BASE COUNT          279 a          543 c          571 g          251 t
ORIGIN      
        1 caacaccgcg cgcttgcaat cgatggccga cgcgcggcgc aaacgcgggg aggagcggca
       61 ggtgctgcag gccggcttcc tggcgctgga cccgcgcaac ggccatgtca aggcctgggt
      121 gggcagccgc gacttcgcgc aggagcagtt cgatcacgtc agccaggcga ggcgccagcc
      181 cggctccact ttcaagccgt tcgtgtacgg cgcggctttc gtggaagggt tccatccgtc
      241 ctccactttc atcgacgaga tcgtggatta ccggtcgccg ggcggcgagg tctggacgcc
      301 cagcgacggg acgccgccca gcggtcagcc catgagcctg cgcgacggcc tggtctattc
      361 caagaacacg atcacggcgc agttgatgcg gcaggtcggg ccggtgcgcg tggcacggct
      421 cgcgcaggcc atgggcgtgc gccagagcaa gctcgacctc gtgccttcgc tcgccctggg
      481 caccagcccg gtgaccttga gggagatggt ggccgcgtac ggcaccatcg cgaactccgg
      541 cacctacacc gagccgctgc tggtgatccg cgtggaagac cggcagggcc gcaccctgga
      601 gggtttcgtt tcgcgccaac ccgagcaggc gatgccgcgt gccagcgcgc tcaccctggt
      661 gaacgtgatg cgcggcgtcg tggacgaggg caccggcgtg gccatccgca cgcgttacgg
      721 catccaggcc gacgtggcgg gcaagaccgg cacaacgcag gacaacagcg acggctggtt
      781 catcctgatg catccgcagc tggtggccgg ggcctgggtc ggcttcaacg acaaccgcat
      841 ggtcatgggc ggccagtggg ggcagggcgc gcgcagcgcg ctgccgatcg tgggcgacta
      901 tttccagcag tcgctgcgct ggcgctggat cgacgcacag gcggagttcg cgattccgcg
      961 gccgcggccc gcaccaccgc cgcagcggcc gcaggacgcg ctgtccgata tcgtgaacga
     1021 cttgctgggg cggatcctgc ggcggctgca gtgagggatc ggcagggccg ctcaagacgc
     1081 tcaagacgct ttgccatcac actcgcggtg cgggacaata ggggccatgg aacgcacggt
     1141 gaccaagacc agactgcgcc gagaggcagg ggacgcccca gcagcggaac tcgcttattg
     1201 gctgagccag ccaatccagg ctcgattggc ggcagtggag acacttcgtc tgcaatacat
     1261 gcggacactt ccggatgctg accaaagact tcaaagagtt tgccgagtta ctcaactcaa
     1321 acagcgttga gtacctgatc gtcgggggat acgcgctggc ggcctacggc cacccgcggt
     1381 acacgggcga cctcgacgcc cggctctttc aatgccgcgc gcggtgcgga tgttccatga
     1441 agaaacgcac catttcgcca gtggcatccg gcccgcgcgg atcggtataa gagcctttgg
     1501 cgctgccgcc ggaccaggcg tgctgcgcgc cgtgcactac ccaatgctcg gcgacgacct
     1561 cgccgccggg gccgcggtgc acgctacggg agtagccgcg gccacccgcg gaaactcctt
     1621 tgtcggtttc actgtggccg gtgc
//
LOCUS       DASWIY010000120         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1173464, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000120 DASWIY010000000
VERSION     DASWIY010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173464"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1360)
                     /locus_tag="VGA59_04665"
     CDS             complement(107..1360)
                     /locus_tag="VGA59_04665"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9719002.1"
                     /translation="MRVEYERCCGLDVHKETVVACVIVPDAAGRAAKETRTFGTMTEE
                     LRALAAWLRAQGVTQVAMESTGVYWKPIFNVLEGEFDLLVVNAEHIKKVAGRKTDVSD
                     AEWIADLLRHGLLRGSFIPSAQQRAWRDLTRYRTRLVDERTSEVNRAQRAPEKVLEDA
                     NLKLASVATDIMGLTGRLILQQLIAGETDPATLAQLAQGRLREKRALLEQALQGKVTP
                     HHRFMLVEHLSHIDYLDEAIGRLDAEIEEQMRPFEEVLARWDQVPGIGPRIAQIMVAE
                     VGVDLKQFEDAAHLASWAGLCPGNQESAGKRKGGKTRKGSRWLRRALTEAAHGAARTK
                     HKYYQAMYHRLVGRRGKKRAIVAVAHSLLVTGYYLITRKRDYQDLGHNYFDERQRDAV
                     KRRAVRRLEQLGFQVSLSPASEPVI"
BASE COUNT          302 a          557 c          500 g          352 t
ORIGIN      
        1 tgaatgcttg agctgcggta acacttgacg atgcactgcg actgtaccaa ctgctacgaa
       61 tcgtcattcg ttcctcctgg gacgttctcc cttgaaaatg atagggtcag atgactggct
      121 ccgaggcggg actgagggac acctggaagc cgagttgttc gagccggcgc acggcgcgac
      181 gtttgacggc gtcgcgctga cgttcatcaa agtagttgtg gccgaggtcc tggtaatccc
      241 gcttgcgggt gatgagatag tagccggtga cgaggagact atgcgctacg gccacaatgg
      301 cgcgcttctt cccccggcgt ccgaccaggc ggtgatacat ggcctggtag tatttgtgtt
      361 tggtgcgggc cgcgccgtgg gcggcttcgg tcagggcgcg gcgcaaccaa cgactgcctt
      421 tgcgcgtctt gccgcctttg cgtttgccgg cgctttcctg gttacccgga cagagaccgg
      481 cccaggaggc caggtgggcg gcatcctcga actgcttgag gtcgacgccc acttccgcta
      541 ccatgatttg ggcgatgcgg ggaccgatgc ccggcacctg atcccagcgc gccaggacct
      601 cttcaaaagg gcgcatctgt tcctcaattt cggcatccag ccggccaatc gcttcgtcca
      661 gatagtcgat gtggctgagg tgctcgacca gcatgaagcg atgatgcggc gtcaccttgc
      721 cctgcaacgc ttgttccaac aaggcgcgct tctcgcgcag gcgtccctgc gccaactgcg
      781 ccagcgtggc agggtcggtc tcgccggcga tcagttgctg caagatcagg cgtcccgtca
      841 accccatgat gtcggtggcg acactcgcca gcttgaggtt ggcgtcctcc aacaccttct
      901 cgggagccct ttgggcgcgg ttgacttcgg aggtgcgttc gtcgaccaag cgagtccgat
      961 accgcgtcag gtcgcgccag gcgcgctgct gcgccgacgg aatgaaactg ccgcgcaaca
     1021 gaccgtgacg caacaggtcg gcgatccatt cggcatcgga cacatcggtc ttgcgtcctg
     1081 ccaccttctt gatgtgctcg gcattgacca ccaacaggtc gaactcgcct tccaacacgt
     1141 tgaagatcgg cttccaatag acgccggtgc tttccatcgc cacctgcgtg acgccctggg
     1201 cacgcaacca cgccgccagc gcccgcaact cttcggtcat ggtcccaaag gtgcgcgtct
     1261 ccttggccgc ccgacccgcc gcatcgggca cgatgacgca cgccacgacc gtctctttat
     1321 gcacatccag cccacaacaa cgttcatatt cgactcgcat ggttacctcc gctcgatgcc
     1381 aaaatcttct cgccggcatc agcggagtgc cccttatgca aggattctcc cttacgtcct
     1441 cacccatcgc tggggggaac agaccgtggt accccaggac actcggatca aacttctcaa
     1501 cgggttcgtg ataccagcct gccgcgatcc ccgacgctga tgccgccacg tccattttca
     1561 tccgaccgct ggtgtacggc aagtacatgg agaaacttct cacgatgttg acacccgtca
     1621 ctgcggtctt gctcatgttg ctcgtcatca cgccggacgg ctatacccgc gcgggctggc
     1681 aaatgctggg cttgcaccgg ctgggcctgc g
//
LOCUS       DASWIY010000121         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3036034, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000121 DASWIY010000000
VERSION     DASWIY010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3036034"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VGA59_04670"
     CDS             complement(<1..231)
                     /locus_tag="VGA59_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9719003.1"
                     /translation="MRAALTHLGAAVTAGIWRLGYGARFFAYLLVHSGTAFRRFRLTL
                     REIYFAGVLSLIIILVSGLFVGMVLGLQGYDTL"
     gene            298..1272
                     /locus_tag="VGA59_04675"
     CDS             298..1272
                     /locus_tag="VGA59_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX9719004.1"
                     /translation="MNKPTQSTFAPAGFEAFRKVTRREKFLAEMDRVVPWVELVALVK
                     PAYAKGTSGPGRPPIPLERMLRIYFLQHWFNLSDPAVEEALYDVSSMRAFVGIDLGRE
                     SAPDETTVCKFRHLLEEHGLGKRIFEAVGAHLQRQGLKVSSGTIVDATIIAAPSSTKN
                     ADGERDPEMHQTRKGKDWHFGMKAHVGVDKKTKLIHSVVATPANIADGNVVGDLLHGG
                     ETHVWGDAAYSGRTERIRAKAPKAKDFTQRRGRGYRYLSEYDRWINRARSRVRARVEH
                     SIGVIKRVFGFAKVRYRGIAKNANRLFVTCALANLFIARHRLRRVQGA"
     gene            complement(1417..>2087)
                     /locus_tag="VGA59_04680"
     CDS             complement(1417..>2087)
                     /locus_tag="VGA59_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9719005.1"
                     /translation="QNDDYGKDYLKGFRDGLGDKAASMIVKEVSYEVTDPTVDSQMVQ
                     LQASGADTFFNITTPKFAAQAIRKAYDTGWKPTHYLNNVSTSIAGVLKPAGLDKSDGL
                     ISTQYYKDPNDGQWKNDPAVLEWKSFMRKYYPEGNVADNANIYAYAVAQTMHQVLKQS
                     GDNLTRANIMKQAASLKDFRPDTLLPGIRINTGASDFAPIESEQLIRFNGQQWERFGP
                     IIGR"
BASE COUNT          394 a          624 c          680 g          389 t
ORIGIN      
        1 cagggtgtcg tagccctgca ggccgagcac catgccgacg aacaggccgc tgaccaggat
       61 gatgatcagc gaaagcacgc cggcgaagta gatctcgcgc agcgtgaggc gaaagcgccg
      121 gaacgcggtg cccgagtgca ccagcaggta ggcgaagaag cgcgcgccgt agcccaggcg
      181 ccagatgccg gcggtcaccg ccgcgccaag gtgcgtgagt gcggcgcgca tcggctaggg
      241 aaactctgaa gtaatcctct gctctaatgc caagtagctg agccgaggag aacaagaatg
      301 aacaagccga ctcaatcgac gtttgcgcca gcggggtttg aggcattccg caaggttacg
      361 cggcgcgaga agttcttggc cgagatggat cgggtggtgc cctgggtgga gctggtggcg
      421 ctggtgaagc cggcctacgc caagggtacg agcggtccgg gccggccgcc gattccgctg
      481 gagcggatgc tgcgcattta ctttctgcag cactggttca acctgtctga cccggcggtc
      541 gaggaagcgc tctacgacgt gtcctcgatg cgcgccttcg taggcattga ccttgggcgc
      601 gagtcggcgc cggacgagac cacggtgtgc aagttccggc acctgctgga ggagcacggt
      661 ctgggcaagc ggatcttcga ggccgtgggg gcgcacctgc agcgccaagg gctgaaggtc
      721 tcctcgggca cgatcgtgga tgcgaccatc attgccgcgc ccagctcgac caagaacgcc
      781 gatggcgaac gcgacccgga gatgcaccag acgaggaaag gaaaagactg gcacttcggc
      841 atgaaggcgc acgtgggcgt ggacaagaag accaagctga ttcactcggt cgtggccaca
      901 ccggccaata ttgccgacgg caacgtggtg ggcgatctgc ttcacggagg cgagactcac
      961 gtctggggcg atgccgccta ctcgggccgc accgaacgca tccgagccaa ggcgcccaaa
     1021 gccaaggact tcacccagcg ccgtggccga ggctaccggt acctgagcga gtacgaccgc
     1081 tggatcaacc gcgctcgctc cagagtccgg gcccgcgtcg agcactcgat cggcgtgatc
     1141 aagcgtgtgt tcggcttcgc caaggtgcgc taccgcggca tcgcgaagaa cgccaaccgc
     1201 ttgttcgtca cctgtgcgct ggccaacttg ttcatcgccc gacatcgatt gcgcagagtt
     1261 cagggggcgt agtgcgtcca gaaacagcga agaagcttca aacgaagcca aatcgggcac
     1321 caacggagcc cgattcgcca tcatccggtg ctcgtgagct cgcgttcgca tcaatgaccg
     1381 ctctcgtcgc cgttacttca gacgttccct aggcggctac ctgccgatga tcgggccgaa
     1441 gcgctcccat tgctggccgt taaaccggat caactgctcc gactcgatcg gcgcgaagtc
     1501 gctggcgccg gtgttgatgc ggattccggg cagcagagtg tccggccgaa agtccttcag
     1561 gctcgcggcc tgcttcatga tgttggcgcg cgtcaggttg tcgcctgact gttttagcac
     1621 ttggtgcatg gtctgggcta cggcgtaggc gtagatgttg gcgttgtcgg caacgttgcc
     1681 ttccggatag tacttcctca tgaacgactt ccactccaat accgccggat cgttcttcca
     1741 ttggccatca ttgggatctt tgtagtattg cgtcgaaata agaccgtcgg acttgtctag
     1801 ccctgccggc ttcagcacgc cagcgatgga ggtcgacacg ttgttaagat agtgcgtcgg
     1861 cttccagccg gtgtcgtagg ccttgcggat cgcctgcgcg gcgaacttgg gcgtggtgat
     1921 gttgaaaaac gtatccgcgc ccgaagcctg cagctgcacc atctgggagt cgaccgtggg
     1981 gtcggtcacc tcgtaggaca cttccttgac gatcatgctc gcggccttgt cgccgaggcc
     2041 gtccctgaag cccttgaggt agtccttgcc gtagtcgtcg ttctggt
//
LOCUS       DASWIY010000122        10592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1174167, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000122 DASWIY010000000
VERSION     DASWIY010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10592
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174167"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VGA59_04685"
     CDS             <1..1163
                     /locus_tag="VGA59_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9719006.1"
                     /translation="RSQLPAALALNGVAMNASRIVGPLVAGAIIAAAGSAWVFVLNAA
                     LSLTAAFVIMRWRREHKESPLGRERLASAMRVGVQFVWQSARMRAVLLRISLFFLHST
                     ALLALLPLVARSLPGGAAGTFTILLAAMGAGAIVAALLMVRIRALVPAQALLLGGTAL
                     QSAGAVIVAFAPNIYIAVPAMVLAGVAWITVANSLTVSAQMALPDWVRARGMSIYQMA
                     LMGSTAAGAALWGQIATWTNIHDALAIAAVSSIALMALAQRLVVDRGIEEDLTPSRVF
                     KVPHAEAPPKSGRIQANIEYHIDPARSAEFLELMQESRRSRMRQGALDWQLLRDLYDP
                     GRFVEQITDESWTEHLRRFDRVTAADVQLRDRKLAFHIGAEPPLVTRLLIER"
     gene            complement(1226..1855)
                     /gene="plsY"
                     /locus_tag="VGA59_04690"
     CDS             complement(1226..1855)
                     /gene="plsY"
                     /locus_tag="VGA59_04690"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX9719007.1"
                     /translation="MQTLYPILAAIAAYLIGSISFAVIVSRVMGLKDPRTFGSRNPGA
                     TNVLRSGSKAAAVVTLSLDAVKGFLPVFLVMRFGKPYGLGDGTIAMVGLAAFLGHLWP
                     VFFRFKGGKGVATFIGVVFGINVLLGIATAATWLIIAFFFRYSSLASLVSAAFSLAYY
                     LLGSRVQWYADARIAIALFAMAMLLAYRHRENISRLLQGKESRLGAKKA"
     gene            complement(1876..2367)
                     /locus_tag="VGA59_04695"
     CDS             complement(1876..2367)
                     /locus_tag="VGA59_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEX9719008.1"
                     /translation="MGKKDHISETPATSFLKANKVAFTEHPYEYLEHGGAQHSAQMLG
                     FDPFTVVKTLVMEDEKGRPLLVLMHGNRKVSTKNLARQIGVKSVEPCKPEVANRHSGY
                     LVGGTSPFGARKAMPVYIEETILALPRIAINGGRRGYLVGLDPQVCARLLDAKPVNCA
                     LAE"
     gene            complement(2551..4458)
                     /locus_tag="VGA59_04700"
     CDS             complement(2551..4458)
                     /locus_tag="VGA59_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9719009.1"
                     /translation="MGVSQGRDNHARMHAGFGWQVPEYFNIAQVCCRRWAQRPGAIQR
                     TAIRAHGARSGAEYLSYLELQARANALSNLLAALGVQRGDRVAIVMPQRFETAIAYMA
                     VFQMGAVAMPLSILFGPDALEYRLQDSEAVVAVCDESSIANLLAMRGECPALRTIVGV
                     GAGASQADLDYASELARQPTGFTAVQTKADEGAVLIYTSGTTGPPKGAFIPHRALIGN
                     LPGFVCSQNWFGFATGLSSRPPSRDPSRRADRASSSKSWIPDQVRDDMGVVIAGSDPQ
                     SRKSSIPDRVRDDSNAVFWSPADWAWTGGLMDALLPTLYFGRPIVAYSGRFSPEVALT
                     LMQEHAITHTFLFPTALKAMMKAFPEPGRQFRLNLQAIMSAGEAVGDAVFAYCRDHLG
                     VTVNEMFGQTEINYVVGNCSMNGDTGKGIGWPARPGSMGRPYPGHRVAVIDDAGNECP
                     AGVAGDVAVNRHDVHGDPDPIFFLGYWKNDSATRGKYTGDWCRTGDLATRDEDGYLWY
                     QGRADDVFKAAGYRIGPSEIENCLVKHPAVINAAVVPKPDRERGALVKAFVVLAPDFA
                     VARAQSAPETAGFDAQLVAQLQSHVKGKLAPYEYPKEIEFIDALPMTTTGKVQRRVLR
                     LQEEEKAKALS"
     gene            4486..5394
                     /locus_tag="VGA59_04705"
     CDS             4486..5394
                     /locus_tag="VGA59_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9719010.1"
                     /translation="MYQPKRSPRSEYVPIRNLKYHVLVWGEPGPGKVPLVMVHGWMDV
                     AASYQFVVDAFAQDHYVIAPDWRGYGLTQSPDADNYWFPDYLADLDFLLDHYSPDAPV
                     HLVGHSMGGNVAMLYAGSRPQRVRRLVNLEGFGMPATHAAQAPKRYAHWMDELKRFHR
                     GELDLKRYESVAGVASRLMKTNKRLTQDKADWLARHWAKPDPDGMWRILGDPAHKIVN
                     AQLYRVDEVIEIYRSITAPTLDVRSSEDNLSHWWKGKYTLAEYHKRVGAVISLHDAVV
                     EDAGHMLHHDQPEKLATLLEEFIVTI"
     gene            5672..6439
                     /locus_tag="VGA59_04710"
     CDS             5672..6439
                     /locus_tag="VGA59_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719011.1"
                     /translation="MRVRIHGLNAPAHREYTMQALRAVTAEAGIKLVDVSDQPDAEQV
                     SNVTVEITADTQLEDNQPCVTFLDFRTETRIDSAVVQMRSRDARRCAYHEAMHVMGVR
                     GHPSGKTVLSYFPWKIDGLMELDKVMLRAWYSPRMTGGMTPFEALPVLADELVATAPN
                     KAAAAQARDVFFSTTIQKMHAYANGQGDVPAVVKRSGKATAEGIRYGRGEMSYFLGIA
                     YMEGATAPRDTSLAVRWLQRAATMGNRGAQAKLGGFR"
     gene            6538..7642
                     /gene="prfB"
                     /locus_tag="VGA59_04715"
     CDS             join(6538..6618,6620..7642)
                     /gene="prfB"
                     /locus_tag="VGA59_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805914.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX9719012.1"
                     /translation="MEPERINLIGGQLVDLAARTEALRGYLDFDAKSERLRTVNASLE
                     DPQVWNDPKKAQELGREKKQLDGVVGMLSNLARELSDNVELFEMSKEEGDEAGLITIE
                     AEAAKLASDVEALEFRRMFNNPADPLNAFLDIQAGAGGTEACDWASMLLRQYLKYAER
                     KGFKASVEDETPGDTAGIKSATIKLEGDYAYGLLRTETGVHRLVRKSPFDSAGGRHTS
                     FASVFVYPEVDDSIEIDINPADVRVDTYRASGAGGQHINKTDSAVRLTHLPTNIVVQC
                     QDSRSQHSNRDVAWKRLRSRLYDHEMRKRMEEQQKLEDTKTDVGWGHQIRSYVLDNSR
                     IKDLRTGVEISNTQKVLDGDLDPFIEASLKQGV"
     gene            7849..10503
                     /gene="pepN"
                     /locus_tag="VGA59_04720"
     CDS             7849..10503
                     /gene="pepN"
                     /locus_tag="VGA59_04720"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX9719013.1"
                     /translation="MREGQDNVIYRADYQAPAFWIDTVELCFDLDPAKTRVLSKMKLR
                     RNPDVVPQPLRLDGEELNLARVLVDGQGTSFKLENNQLVLENLPEGYEPFELEIFTTC
                     APAKNTKLMGLYVSNDSFFTQCEAEGFRRITYFLDRPDVMASYTVLLRADKQKYPVLL
                     SNGNLVDQGELEQGRHYAKWVDPFRKPSYLFALVAGQLVAREQRITSRGGREHLLQVY
                     VRPGDLDKTEHAMNSLMASVAWDEARFGLSLDLERFMIVATSDFNMGAMENKGLNIFN
                     TKYVLASQATATDTDFGNIESVVGHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQEF
                     SQDLAGAPSARAVKRIEDVRVLRTAQFPEDAGPMAHPVRPDQYLEINNFYTVTVYEKG
                     AEVVRMMQTLVGREGFAKGMTLYFQRHDGNAVTCDDFAQAIADANPDSRLAQLLPQFK
                     RWYSQAGTPHVSATAAYDADSRNYTLTLSQSCPATPGQSEKEPFVIPIGLGLLAPDGS
                     ELTTGMHVLTQASETLSFPGLQAEPVPSILRGFSAPVILEFGYSEEQLLTLLAHDSDP
                     FNLWEAGQRLAVNRAIRFIGQSELTAGDIRLDEPYLQAMRSILRHPQLDAAFKELVLT
                     LPAETYIAEQLEVVDPQRIHAVREAMREQLAQALFDDWAWAYDAHRDSGAYRPDPVSS
                     GRRALAGLALTHLCLAARRSRDTVWPGKAFQRFKDAGNMTDRFNALAALVSSGHELAV
                     PALQRFHSLFSNEPLVLDKWFALQAGAPDRGGNVLPAVKQLMNHPDFHIRNPNRARSL
                     IFSYCSANPGAFHRTDAAGYVFWGDRVIELDAINPQVAARLARALDRWNKLVEPLRGA
                     AREAIARVAAKPDLSNDVREVVTRALGD"
BASE COUNT         1892 a         3534 c         3391 g         1775 t
ORIGIN      
        1 cgagatcgca gctgccggcc gcgctggcgc tcaatggggt ggcgatgaac gcgtcgcgca
       61 tcgtcgggcc gctggtggcg ggcgcgatca tcgccgccgc cggcagcgcc tgggtgttcg
      121 tgctcaacgc cgcgctctcg ctgacggcgg ccttcgtcat catgcgctgg cggcgcgagc
      181 acaaggaaag ccccctgggt cgcgaacggc tggccagcgc catgcgggtg ggcgtccagt
      241 tcgtctggca atcggcgcgg atgcgtgcgg tgctgctgcg catctcgctg ttcttcctgc
      301 attccacggc cctgctcgcg ctgctgccgc tggtggcgcg cagcctgccg ggcggcgcgg
      361 ccggtacctt caccatcctg ctggcggcaa tgggcgcggg cgccatcgtg gcggcgctgc
      421 tcatggtgcg cattcgcgcc ctggtgccgg cgcaggcctt gctgctcggg ggcacagcgc
      481 tgcagtcggc cggcgccgtc atcgtggcgt tcgcgcccaa catctacatc gcagtgccgg
      541 ccatggtcct tgccggcgtg gcctggatca cggtggcgaa ctcgctcacc gtgtcggcgc
      601 agatggcgct gcccgactgg gtgcgtgccc gcggcatgtc gatctaccag atggcactga
      661 tgggctcgac cgcggcgggc gccgcgctgt ggggccagat cgccacctgg accaacatcc
      721 acgacgccct ggcgatcgcg gcggtctcca gcatcgccct gatggcgctg gcgcagcggc
      781 tggtggtgga ccgcgggatc gaggaagacc tgacgccctc gcgcgtcttc aaggttcccc
      841 atgccgaggc gccgccgaaa tcgggccgca tccaggccaa catcgaatac cacatcgatc
      901 ccgcgcgctc ggccgaattc ctggagctga tgcaggagag ccgccgcagc cgcatgcgcc
      961 agggcgccct cgactggcag ttgctgcgcg acctgtacga cccgggccgg ttcgtggaac
     1021 agatcacgga cgagtcatgg accgagcacc tgcgccgctt cgaccgcgtc accgctgcgg
     1081 acgtgcagct gcgcgatcgc aaactggctt ttcacatcgg ggcggagccg ccgctggtga
     1141 cgaggttact gatcgagcgc tagaaaatgc gcgcaagaac ctcgcagcgc tccggcaggc
     1201 cgcatcgccc gacgcgggcc gccggctagg ctttcttcgc ccccagcctc gattccttcc
     1261 cctgcagcag ccgcgagatg ttctcgcgat gccggtaggc gagcagcatg gccatcgcga
     1321 acaaggcgat cgcgatccgg gcgtccgcgt accactggac gcggctaccc aggagatagt
     1381 aggcgagcga gaacgcggcc gagacgagcg aggccagcga cgagtagcgg aagaaaaagg
     1441 caatgatcag ccaggtcgcg gcggtggcga tgcccagcag cacgttgatg ccgaaaacga
     1501 cgccgatgaa agtggccacg cccttgccgc ccttgaacct gaagaacacc ggccacaggt
     1561 gaccgaggaa agcggccagg cccaccatcg cgatcgtccc gtcacccagg ccgtagggct
     1621 tgccgaaccg catcaccagg aacaccggca agaagccttt caccgcatcg agcgatagcg
     1681 tgacgacggc cgcggccttg ctgcccgagc gcagcacatt ggtggcgccc ggattcctgc
     1741 tgccgaaggt gcgcgggtcc ttcaggccca tcacgcggct gacgatcacg gcgaacgaga
     1801 tcgagccgat caggtaggcc gcgatggcgg ccaggatggg ataaagcgtt tgcaagcggt
     1861 gcctccggct aggccctatt ctgccagcgc gcaattcacc ggcttggcat cgagcaggcg
     1921 cgcgcaaacc tggggatcca ggcccaccag gtagccgcgc cggccgccgt tgatcgcgat
     1981 cctgggcagc gccagaatcg tctcctcgat gtacaccggc atcgccttgc gcgcgccgaa
     2041 cggcgacgtg ccgcccacga ggtagccgct gtggcgattg gccacttcgg gcttgcaggg
     2101 ctcgaccgac ttcacgccga tctggcgcgc cagattcttg gtcgatacct tgcggttgcc
     2161 gtgcatcagc accagcagcg gcctgccctt ttcatcctcc atgaccagcg tcttcacaac
     2221 ggtgaacgga tcgaacccga gcatctgggc gctgtgctgc gcaccgccat gctccaggta
     2281 ttcatacggg tgctcggtga aagcgacctt gttcgccttg agaaacgagg tcgcgggggt
     2341 ttcgctgatg tggtccttct tgcccattgc ccttgcctat gcccttgccc ttgcccttgc
     2401 ctatgcctct gccttgtcat tgcggactcg atccgcaatc catggatccc ggatcgggcc
     2461 tgccccggac ttgatccggg gtccgggatg acagccccct gccccggatg gcgagtccac
     2521 gccccgggat ccaggtattt gtcggcgcgc ctatgacagc gccttggcct tttcctcttc
     2581 ctgcaatcgc agcacccgcc gctggacctt gcctgtggtg gtcatcggca aggcgtcgat
     2641 gaactcgatt tccttcgggt attcgtaggg cgcgagcttg cccttcacgt gcgactgcag
     2701 ctgggccacc agctgcgcgt cgaagccggc ggtctccggc gccgactgcg cgcgcgccac
     2761 ggcgaagtcg ggcgccagca cgacgaaggc cttgacgagc gcaccgcgct ctcggtcggg
     2821 cttgggcaca acggctgcgt tgatcacggc cggatgcttg accaggcagt tctcgatctc
     2881 gctcggtccg atgcggtagc cggcggcctt gaagacgtcg tcggcacggc cctggtacca
     2941 cagatagccg tcctcgtcgc gcgtggccag gtcgccggtg cggcaccagt cgccggtgta
     3001 cttgccgcgg gtggccgaat cgttcttcca gtagcccagg aagaagatcg gatcggggtc
     3061 gccatggacg tcgtggcggt tcacggcgac gtcgcctgcg acgcccgcgg ggcattcgtt
     3121 gccagcgtcg tcgatgacgg ccacccggtg gccggggtaa ggccgcccca tgctgccggg
     3181 cctggcgggc cacccgatgc ctttgccggt atcgccgttc atggagcagt tgccgaccac
     3241 gtaattgatc tcggtctggc cgaacatctc gttcaccgtg acgccgagat gatcgcggca
     3301 gtaggcgaac acggcgtcgc ccacggcttc gccggcgctc atgatggctt gcagattcag
     3361 ccggaactgc cggcccggct cggggaaggc tttcatcatc gccttgagcg ccgtagggaa
     3421 caggaaggta tgcgtgattg cgtgctcctg catcagcgtc agcgccacct ccggactgaa
     3481 gcggccgctg taggccacga tcggccggcc gaagtacagc gtgggcagca gcgcatccat
     3541 caacccgcct gtccaggccc agtccgccgg ggaccagaac accgcgttgc tgtcatcccg
     3601 gactcgatcc gggatcgatg acttcctgga ttgcgggtcg gagcccgcaa tgacaactcc
     3661 catgtcatcc cggacttgat ccgggatcca tgacttgctt gacgaagcac ggtctgcccg
     3721 tcgcgatgga tcccggctcg gtggccggga tgacaagccc gtggcgaacc cgaaccagtt
     3781 ctggctgcac acgaagcccg gcaggttgcc gatcagcgca cgatggggaa tgaaggcgcc
     3841 cttgggcggg ccggtggtgc cgctggtgta gatcagcacc gcaccttcgt ctgccttggt
     3901 ctgcacggcg gtgaagccgg tcggctgcct ggccagttcg ctcgcatagt ccaggtcggc
     3961 ctgtgacgcg cctgcgccga cacccacgat cgtgcgcaag gccggacact cgccgcgcat
     4021 ggcgagcagg ttggcgatcg aactttcatc gcacacggcc actacagcct cgctgtcctg
     4081 caggcggtat tcgagcgcgt cgggcccgaa caggatcgac agcggcatgg ccacggcgcc
     4141 catctggaat accgccatgt acgcaatcgc ggtctcgaag cgctgcggca tgacgatcgc
     4201 cacacggtcg ccgcgttgca cgcccagcgc cgccagcagg ttgctcagcg cgttggcgcg
     4261 ggcctgcaat tcgaggtagg acaggtattc cgcgccggac ctggcgccat gggcgcgaat
     4321 cgcggtccgt tggatcgcgc ccggtcgctg cgcccagcgc ctgcagcaga cctgcgcgat
     4381 attgaagtac tcgggtacct gccagccgaa gccggcgtgc atgcgcgcgt gattgtccct
     4441 gccttgactg acgcccatca cagtgcctcc ttatgatccc cggcaatgta ccaacccaaa
     4501 agatctcccc gcagcgagta tgtcccgatc cgcaacctga aataccacgt gctggtctgg
     4561 ggcgagcccg gcccggggaa ggttccgctg gtcatggttc acggctggat ggacgtagcc
     4621 gcttcctacc agttcgtggt ggacgccttc gcgcaggacc attacgtgat cgcgcccgac
     4681 tggcgcggct atggcctgac gcagtcaccc gacgccgaca actactggtt ccccgactac
     4741 ctggccgacc tggatttcct gctcgaccat tactcccccg atgccccggt ccatctcgtt
     4801 ggccacagca tgggcggcaa tgtcgccatg ctttatgccg gatcgcggcc gcagcgcgtc
     4861 cgccgcctgg tcaatctgga aggcttcggc atgccggcga cgcacgctgc ccaggcaccg
     4921 aaacggtatg cccattggat ggatgagctc aagcgcttcc atcgcggcga gctggacctc
     4981 aagcgctacg agtcggtggc gggagtggcg agccgcctga tgaagacgaa caagcggctc
     5041 acgcaggaca aggccgactg gctggctcgg cattgggcca agccggatcc ggacggcatg
     5101 tggcgcatcc tgggagaccc cgcccacaag atcgtcaatg cccagctgta ccgtgtcgat
     5161 gaagtcattg agatctatcg gtcgatcacg gcgccgacgc tggacgtccg atccagcgag
     5221 gacaacttga gccactggtg gaagggtaag tacacactgg cggagtacca caaacgagtg
     5281 ggagccgtca tcagtttgca cgatgcggtg gtcgaggatg cgggccatat gctgcaccac
     5341 gatcagcccg aaaaactcgc gactttgctt gaggagttca ttgtaaccat ttgatattgc
     5401 tgcgttaatg caacaaatta ccgctgaatt gattttccgc aagctccagt aggcaaaatc
     5461 cctagacgta ggacgcctcc taccctcaca cttgagccac aaattgtgct cgaggtgtcg
     5521 gatgcgtcga ttttgttctg gactgttgct ggcagctctg ctgcctttct cagccatggc
     5581 cgaggacttc gatcaagggc tcgcgaccct gtgggaagtt ctttggcacc aaagtggtac
     5641 cccgacacgg gtcgtgcgct gggaaagcga aatgcgcgtg cgcatccatg gcctgaacgc
     5701 tccagcccat cgggagtaca ccatgcaggc gctgcgcgcg gttaccgccg aggccggcat
     5761 caagctggtc gatgtgagcg accagcccga tgcagagcag gtttcgaacg tcaccgttga
     5821 aatcaccgcg gatacccagc tcgaggacaa ccagccctgt gtcacctttc tggacttccg
     5881 caccgaaacc aggatcgact cggcagtggt gcagatgcgc tcgcgcgatg cgcggcgctg
     5941 cgcctaccac gaggcaatgc atgtgatggg cgtgcgcggc catccctccg gtaagaccgt
     6001 gttgagttac ttcccctgga agatcgacgg attgatggag ctcgacaagg tgatgctgcg
     6061 cgcctggtat tcgcccagga tgaccggcgg catgacaccg ttcgaggcgc tgcccgtgct
     6121 ggcggatgag ctggtggcca ccgcacccaa caaggcggcc gccgcgcagg cgcgcgacgt
     6181 gttcttctcg accaccatcc agaagatgca cgcctacgcc aacggccagg gtgacgttcc
     6241 cgccgtggtc aagcgctccg gcaaggcaac cgccgagggc atacgctatg ggcgcggcga
     6301 gatgagctac ttcctgggca ttgcctatat ggaaggcgca acggctccgc gcgatacgag
     6361 cctggcggtc cgctggctgc aacgcgcagc caccatgggc aaccgcggcg cgcaagccaa
     6421 gctcggcggc ttccgctaac cgctacgctc caacacgaaa cgcgccgcct gcggcggacg
     6481 cgtccttggg cggcttgcgc cgtgtcagaa tccttggttc acgaggattt tttcattatg
     6541 gaaccagaac gcatcaacct gatcggcggc cagctcgtcg atctcgccgc ccgcaccgaa
     6601 gccctccggg ggtatctttg acttcgatgc caagtcggag cgcctgagga ccgtcaacgc
     6661 ctcgctcgaa gatccccagg tctggaacga tcccaaaaaa gcccaggagc tgggccgcga
     6721 aaagaagcag ctcgatggcg tcgtcggcat gctgtcgaac ctcgcccgcg agctttcgga
     6781 caacgtcgag ctctttgaaa tgagcaagga ggaaggcgac gaagcgggcc tcatcacgat
     6841 cgaagcggaa gcggccaagc tcgccagtga tgtggaggcg ctggagttcc gccggatgtt
     6901 caacaacccg gcggacccgc tgaacgcctt cctggacatc caggcgggcg cgggcggcac
     6961 ggaagcttgc gactgggcca gcatgctgct gcgccagtac ctgaagtacg ccgagcgaaa
     7021 gggcttcaag gccagcgtcg aagacgagac gcccggcgat acggccggca tcaagagcgc
     7081 caccatcaag ctcgaaggcg actacgccta tggcctgctg cgcacggaaa cgggcgtgca
     7141 ccgcctggtg cgcaagtcgc ctttcgattc cgcgggcggg cggcacacca gcttcgccag
     7201 tgtgttcgtc tatcccgagg tcgacgattc gatcgagatc gacatcaacc cggcggacgt
     7261 gcgggtggat acctaccgcg ccagcggcgc gggcgggcag cacatcaaca agaccgattc
     7321 ggccgtgcgc ctgacgcatt tgccgaccaa catcgtcgtg cagtgccagg acagccgcag
     7381 ccagcacagc aaccgcgacg tggcctggaa gcgcctgcgt tcgcgcctgt acgaccacga
     7441 gatgcgcaaa cgcatggaag agcagcaaaa gctcgaggac accaagaccg acgtcggctg
     7501 ggggcaccag atccgcagct acgtgctgga caacagccgc atcaaggacc tgcgcaccgg
     7561 cgtggaaatc tccaacaccc agaaggtgct ggacggcgac ctcgatccgt tcatcgaagc
     7621 gtcgctgaag cagggcgtat gagatcgccc agcgtcatcg ccgcattgac ggctgtcatt
     7681 gcggactcga cccccaatct acccgcctct gtcattgcct ctgtcattgc gggcttgacc
     7741 cgcaatccag gtttcgagcg ccgacatgga tcccgggtcg agcccgggat gacagtctgg
     7801 tgcgcgatga ccatgaactc atgaactcga acttccggga gagcgcgtat gcgtgaagga
     7861 caagacaacg tgatctaccg cgccgactac caggcacccg ccttctggat cgacacggtg
     7921 gagctgtgct tcgacctgga cccggcgaag acgcgcgtgc tgagcaagat gaagctgcgc
     7981 cgcaacccgg atgtcgtgcc gcagccactg cgcctcgacg gcgaggagct gaatctggcg
     8041 cgggtcctgg tcgatggcca gggcacctcg ttcaagctgg agaacaacca gctggtgctg
     8101 gaaaacctgc ccgaaggcta cgagccgttc gagctggaaa tcttcaccac ctgcgcgccc
     8161 gcgaagaaca ccaagctcat gggcctgtac gtgagcaacg attccttctt cacccagtgc
     8221 gaggccgaag ggttccggcg catcacctat ttcctggacc gccccgacgt gatggccagc
     8281 tacacggtcc tgctgcgcgc ggacaagcag aaatacccgg tgctgctgtc caacggcaac
     8341 ctggtcgacc agggtgagct ggagcaaggg cgccactacg ccaagtgggt cgatcctttc
     8401 cgcaagccca gttacctgtt cgcgctggtg gccggccagc tggtggcccg cgagcagcgc
     8461 atcacctcgc ggggcggcag ggaacacctg ctgcaggtct atgtgcgccc cggcgacctg
     8521 gacaagaccg agcacgcgat gaattcgctg atggcctcgg tggcgtggga cgaagcgcgt
     8581 ttcggcctct cgctggacct ggagcgcttc atgatcgtcg ccaccagcga cttcaacatg
     8641 ggcgcgatgg agaacaaggg cctgaacatc ttcaacacca agtacgtgct ggccagccag
     8701 gcgaccgcca ccgataccga cttcggcaac atcgaaagcg tggtgggcca cgagtacttc
     8761 cacaactgga ccggcaaccg cattacctgc cgcgactggt tccagctgag cctgaaggaa
     8821 ggcctcacgg tgttccgcga ccaggaattc agccaggacc tggccggtgc gccctcggcg
     8881 cgcgccgtca agcgcatcga ggacgttcgc gtgctgcgta ccgcgcagtt cccggaggac
     8941 gcggggccga tggcgcatcc ggtgcggccc gaccagtacc tggagatcaa caatttctac
     9001 accgtcacgg tgtacgaaaa aggcgccgag gtcgtgcgga tgatgcagac gctggtcggc
     9061 cgcgagggtt tcgcgaaggg catgacgctg tacttccagc gccatgacgg caatgcggtc
     9121 acctgcgacg actttgcaca agccatcgcc gacgccaatc ccgattccag gctggcgcag
     9181 ctgctgcccc agttcaagcg ctggtacagc caggccggca ctccgcatgt gagcgcgacg
     9241 gccgcatacg acgccgactc gcgcaactac acccttaccc tttcgcaaag ctgccccgcc
     9301 accccgggcc agtccgagaa ggagccgttc gtcatcccga tcggccttgg cctgctcgcg
     9361 ccggatggca gcgaactcac cacgggcatg cacgttctga cgcaagccag cgagacgctg
     9421 agctttcccg gccttcaggc cgaaccggtg ccgtcgatcc tgcgcggctt cagcgcgccg
     9481 gtgatcctgg agttcggcta cagcgaggag cagctgctca ccctgctcgc gcacgacagc
     9541 gatccgttca acctgtggga agccggccag cggctggcgg tgaaccgcgc catccggttc
     9601 atcggccaga gcgaactcac tgccggcgac atccggctcg acgagccgta cctgcaagcc
     9661 atgcgcagta tcctgcgcca tccgcagctc gatgccgcgt tcaaggaact ggtgctcacg
     9721 ctgccggccg aaacctatat cgccgaacag ctggaggtgg tcgatcccca gcgtatccac
     9781 gccgtgcgcg aggcaatgcg cgagcagctg gcgcaggccc tgttcgacga ctgggcctgg
     9841 gcttacgacg cccaccggga cagcggcgcc taccggcccg accccgtgtc gtccggccgg
     9901 cgggcgctgg ccgggttggc actgacgcat ctgtgcctgg cggcgcgccg cagccgcgac
     9961 acggtctggc cgggcaaggc cttccagcgc ttcaaggacg ccggcaacat gaccgaccgc
    10021 ttcaatgccc tggcggccct ggtgagcagc ggccacgaac tggccgttcc ggcattgcag
    10081 cgcttccatt cgctgttcag caacgagccg ctggtgctcg acaagtggtt cgccttgcag
    10141 gccggcgcgc ccgaccgcgg cggcaacgta ttgcccgccg tcaagcagct gatgaaccac
    10201 ccggactttc acatccgaaa ccccaaccgc gcccgcagcc tgatcttcag ctactgcagc
    10261 gccaaccccg gcgcgttcca tcgcaccgac gccgcaggct acgtgttctg gggcgatcgc
    10321 gtcatcgagc tcgacgccat caatccgcaa gtggcggcgc ggctggcgcg cgccctcgat
    10381 cgctggaaca agctggtcga gccgctgcgg ggcgccgccc gcgaggcgat cgcgcgcgtg
    10441 gcggccaagc ccgacttgag caacgacgtg cgcgaggtcg tcacccgcgc gctcggcgac
    10501 tgaaatccat ggaccccggg tcaagcccgg ggtgacagaa tattgtcatt gcgaattttg
    10561 tcattgcggg ctcgacccgc aatccatgtc cc
//
LOCUS       DASWIY010000123         7635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1554765, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000123 DASWIY010000000
VERSION     DASWIY010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554765"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VGA59_04725"
     CDS             complement(<1..495)
                     /locus_tag="VGA59_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9719014.1"
                     /translation="MKLIGSTSSPYVRKVRIVMAEKKLDYEFVTEDVWAADSAISQSN
                     PLGKVPCLVMEGGEALFDSRVIVEYLDTLSPVGKLIPAVGRERAEVKTWEALADGVVD
                     AAILARLESHWAGRGKAQRSQAWIDRQLSKVHASLKAMSQGLGEKPFCAGIYLSLADI
                     AAGCA"
     gene            875..2254
                     /gene="purB"
                     /locus_tag="VGA59_04730"
     CDS             875..2254
                     /gene="purB"
                     /locus_tag="VGA59_04730"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX9719015.1"
                     /translation="MTFTTISALSPLDGRYAGRLAPLRPLMSEQGYMHKRVQVEICWF
                     IALSDAGFAEFKPLSPGARTYLLGLVKNFSEADANAIKEIEKTTNHDVKAVEYWIKSK
                     FEARPELRAAGEFVHFACTSEDINNTSHALQLKGGRDLVLLPALDAVLAKLRQMAYDY
                     ADAPMLSRTHGQTASPTTVGKEIANVVVRLAAAREKIAAVRLLAKMNGAVGNYNAHLA
                     AWPEFDWEAFCRKLVEMPEPLGLGLSFQAYSVQIEPHDYMAELFDAVARANTILIDWS
                     RDVWGYVSLGYFKQRLRDGEIGSSTMPHKVNPIDFENAEGNLGLANALLRHLSEKLPI
                     SRWQRDLTDSTVLRNMGVALGYTALAYHSLGIGLAKLELNEEALAADLDHAWEVLAEP
                     IQTVMRRFGVAGAYEKLKEATRGKTVRAEDLHALIRSLEIPAAEKERLLALTPATYVG
                     KAAELARRC"
     gene            2292..2852
                     /locus_tag="VGA59_04735"
     CDS             2292..2852
                     /locus_tag="VGA59_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEX9719016.1"
                     /translation="MALKATIFRANVAIADIDHAYYADHQLTLARHPSETDERMMIRL
                     AALALNAHRVQTEYGGDATLAFGAGLSNPDDPDVSLRDFTGQTRLWIEVGQPEDKPLA
                     KACGKADHVCVYCFHHAAEVWWRGIEGKLSRLQNLSVFRVPTLASQALAALAQRSMQL
                     QATVHEGTLTLSDAGNSVDLEPLRWK"
     gene            complement(2933..3235)
                     /locus_tag="VGA59_04740"
     CDS             complement(2933..3235)
                     /locus_tag="VGA59_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX9719017.1"
                     /translation="MLRLAQAPNIAIAALWVDALRQAGIAASMQRYYLGAAAGELPPD
                     QCLPEVWLIHDEQETEARAFLADLLHVPQRRWLCQCGEMVEGGFEQCWSCGRLMPR"
     gene            complement(3248..3676)
                     /locus_tag="VGA59_04745"
     CDS             complement(3248..3676)
                     /locus_tag="VGA59_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3717 domain-containing protein"
                     /protein_id="HEX9719018.1"
                     /translation="MAGIHIIDIEAAINFWRTRKPSPDGVMLAPELRALAEVYALLVF
                     RHQEEADERGFPPAAYAAWKTWYETTPDTPCIAICSTSQGDDSCKGCGRSFEEVQHWP
                     AMTPAQKRQVWRRITLRGDALRFNRYSERAAGGPAEPAGA"
     gene            3791..4144
                     /locus_tag="VGA59_04750"
     CDS             3791..4144
                     /locus_tag="VGA59_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX9719019.1"
                     /translation="MKLLAKWLLSAAALLLVAYLYTGVEVKSFPAALIAAFIIGLLNS
                     VVRPILVVLTLPVTIVTLGLFLFIINALMFWAAAGVLDGFHVTGFLAALIGSLIYSLI
                     GVVIESALESLFAQK"
     gene            complement(4112..5500)
                     /locus_tag="VGA59_04755"
     CDS             complement(4112..5500)
                     /locus_tag="VGA59_04755"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX9719020.1"
                     /translation="MNASSERKLGERIARELYRDPDYIDDPVLDEYVQEIWKRLLAAA
                     RARGELTAELDERFAWQVLLGKDRIINAFALPGGWLGLQLGLINVTASRDELASVLAH
                     ELSHVTQRHISRLVTQQNRQAPWVIGAMILGALAASKSPDAANALVSGGQALAIQNQL
                     NFSRDMEREADRIGYGVLTQAGFEPQGFVTMFDKLQQAARLNDNGAFPYLRTHPMTTE
                     RMADMQSRQQLAGRPAASVPDLVHAMMVARARVLSNPGVDGLRSYVTEANGAQATASR
                     ARQVGIVYAAALASSKLRDPAAAARLAGKLAGLTAGDARAARLAGLLAAEVALQAGDS
                     TPAVALDNAAGARPEVLLSSQARVNSGRAAEAAQRLQTWVALHPRDAQAWQLLGAAYT
                     AQGQTLRAIRAEAEVQVAHLDYQAAMDRYKAAQELARKAAAGGGDHIEASIIDTRARQ
                     VESLLREQALER"
     gene            5704..6195
                     /gene="moaC"
                     /locus_tag="VGA59_04760"
     CDS             5704..6195
                     /gene="moaC"
                     /locus_tag="VGA59_04760"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX9719021.1"
                     /translation="MSSLTHFDAQGQAHMVDVGGKTPSRRVAVASGRIEMLPATLAVI
                     ESGTAKKGDVLGVARIAGIQAAKKTSELIPLCHPLALTRLAIDFLIERAPPGGGPAIA
                     CTATAETVGPTGVEMEALTAVQVALLTIYDMCKAVDRGMVITGVRLLEKQGGKSGHYV
                     AGK"
     gene            complement(6233..>7635)
                     /locus_tag="VGA59_04765"
     CDS             complement(6233..>7635)
                     /locus_tag="VGA59_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HEX9719022.1"
                     /translation="AWAAYRLSQARAVQPLHWVQCIAAGWIAAALISSAIALFQYFGL
                     ASYLSPLANATGVGEAFANLRQRNQFASLTAIGMAALLWWAPGTRRGHAVAAMALLAA
                     ANAASASRTGLLQVVMLALFTGLWSGPDRRRRLELCLAAALAYLLAATLLPVLLEAIT
                     GMAPDTIWGRMTRGEGCSSRVVLWSNVLDLISARPWLGWGWGELDYAHYITSYPGARF
                     CAILDNAHNLPLHLAVELGVPAAAIIGGAFLWWVVRAQPWRETDRTRQLAWSVLAVIL
                     LHSMLEYPLWYGPFQLAFVLSLGLLRPSSPSASSNASGHGWWAPGFQRALTLALIAAI
                     AFAAWDYRRISQIYLPPQARDADYRDDTLAHIRGSRLFQDHVRFAELTITPLTRDNAQ
                     WTFDIALALLHYSPEPRVVEKVIESAAMLGRDNEALAHLSRFRAAFPNEYAVWSATQR
                     AAPAPASVAHPTHAGG"
BASE COUNT         1304 a         2561 c         2547 g         1223 t
ORIGIN      
        1 cgcgcaaccg gcggcgatgt cggccagact gaggtagata cccgcgcaaa agggcttctc
       61 gcccagaccc tggctcatgg ccttgaggct ggcgtgcacc ttggacagct gccggtcgat
      121 ccaggcctgg ctgcgctgcg ccttgccgcg gccggcccaa tgggattcca gccgcgccag
      181 gatcgcggca tcgaccacgc catcggccag cgcctcccag gtcttgacct cagcgcgttc
      241 gcggcccacc gccggaatca gtttgcccac cggcgacagc gtgtccaggt attccacgat
      301 cacgcgtgaa tcgaacagcg cttcaccgcc ttccatcacg aggcagggca ccttgcccaa
      361 cgggttcgac tggctgatcg cgctgtcggc agcccatacg tcctccgtca cgaattcgta
      421 gtccagcttc ttttcggcca tgacgatgcg cactttgcgc acgtaggggc tggaggtcga
      481 tccgatcagt ttcatcaaaa gctctctgca atgttcgagt tattgcggtc cattcatttt
      541 agccagggca cacagcacac cctgatccgc gcccggcttg cagcgtaacg ggcgggtttt
      601 ggccgccgcg aggccggctt ttgtggcagg cgcacgacat ggggccgcga cgtggcggcg
      661 cggggccgcg cctacaattg ccggaatgaa tcccccaagc tcgaagtgaa agcccacccc
      721 cgaagcgcct tcggcgatgc cccctcgggc ttcgccctct ccccctcaag ggggcgccac
      781 cagcggcccg gcaaagccgg ttccgcggta gcccgcgcct gcagaatcga ttttcatccg
      841 tcgtgggtga agttctcctt catgggaaac tgaagtgacc ttcacgacga tctccgcgct
      901 ctcgccactg gacggccgtt atgcgggccg gctcgcgccg ctgcgcccgc tgatgagcga
      961 acagggctac atgcacaagc gggtgcaggt cgagatctgc tggttcattg cgttgagcga
     1021 cgccggcttc gccgagttca agccgctcag cccgggcgcc cgcacttacc tgctggggct
     1081 ggtgaagaac ttctccgagg ccgatgcgaa cgccatcaag gagatcgaaa aaaccaccaa
     1141 ccacgacgtc aaggccgtcg aatactggat caagtcgaag ttcgaggcgc ggcccgaact
     1201 gcgagccgcc ggcgaattcg tgcatttcgc ctgcaccagc gaggacatca acaacaccag
     1261 ccacgcgctg cagctcaagg gcgggcgcga cctggtgctc ctgcccgcgc tggacgccgt
     1321 gttggccaag ctgcgccaga tggcgtacga ctacgccgat gccccgatgc tcagccgcac
     1381 gcatggacag accgccagcc ccacgacggt cggcaaggaa atcgcgaatg tggtggtgcg
     1441 cctcgcggcg gcgcgcgaga agatcgccgc cgtgagactg ctggccaaga tgaacggcgc
     1501 ggtgggcaat tacaacgcac acctggcggc ctggcctgaa ttcgactggg aagcgttctg
     1561 ccgcaagctg gtggaaatgc cggaacccct gggcctgggc ctgagcttcc aggcttacag
     1621 cgttcagatc gagccgcacg attacatggc cgagctgttc gacgcggtgg cgcgtgccaa
     1681 cacgatcctg atcgactggt cgcgggacgt ctggggttac gtcagcctcg gctacttcaa
     1741 gcagcgcttg cgcgacggcg agatcggctc ctcgaccatg ccgcacaagg tcaacccgat
     1801 cgatttcgaa aacgccgagg gcaacctggg gctggccaac gccttgctgc gccacttgtc
     1861 cgaaaagctg ccgatctctc gctggcagcg cgacctcacg gattcgaccg tattgcgcaa
     1921 catgggcgtg gctctgggct acacggcact ggcctaccac tcgctgggca taggactggc
     1981 gaaactcgag ctcaatgagg aggcgctggc ggccgatctc gaccacgcct gggaggttct
     2041 ggccgagccg atccagaccg tgatgcggcg tttcggcgtg gcgggcgcgt acgagaagct
     2101 caaggaagcc acgcgcggca agacggtgcg cgccgaagac ctgcatgcgc tgatccgctc
     2161 gctggaaatt ccggcggcgg aaaaggagcg gctgctggcg ctgacaccgg ccacctatgt
     2221 gggcaaagcc gccgaactcg ccagacgatg ctgatggccc ccacgcttcg ctgcccccca
     2281 agggggttcg tgtggcgctg aaggccacca tcttccgggc gaacgttgcg atcgccgaca
     2341 tcgaccacgc ctactatgcg gaccaccagc tgacgctggc gcggcatccg agcgaaacgg
     2401 acgagcgcat gatgatccgg ctggccgcgc tcgcgctcaa tgcccaccgg gtacagaccg
     2461 agtacggcgg cgacgcgacc ctggccttcg gcgccgggct gtccaatccc gacgaccccg
     2521 atgtatcgct gcgcgacttc accggccaga cgcggctgtg gatcgaggtg gggcagcccg
     2581 aggacaagcc gctggccaag gcctgcggca aggccgatca cgtctgcgtt tattgtttcc
     2641 atcatgcggc cgaggtctgg tggcgcggca tcgagggcaa gctctcgcgg ctgcagaacc
     2701 tcagcgtgtt ccgcgtgcct acgctggcct cgcaggcgct ggctgcgctg gcccagcgca
     2761 gcatgcagct gcaggccacc gtccacgagg gcacgttgac gctgagcgac gccggcaaca
     2821 gtgtcgacct ggaaccgctg cgctggaaat agactgatat tgcgatttga cgaaagcttc
     2881 ggacggccga ccggcaggcc ccggccattc gcgcccaggc gtggggccgc gcctagcgcg
     2941 gcatgagccg gccgcagctc cagcactgct cgaagccgcc ttccaccatt tcgccacact
     3001 ggcacaacca gcgccgctgc ggcacatgca gcaggtcggc gaggaaagcg cgggcctcgg
     3061 tttcctgttc atcatggatc agccatacct cgggcaggca ctggtcgggc ggcagctctc
     3121 cggccgccgc gcccaggtaa tagcgctgca tcgacgctgc aatgccggcc tgtcgcagcg
     3181 catcgaccca gagcgcggca atcgcgatat tgggtgcctg ggcgagccgc agcatggcag
     3241 gccctgctca ggcgccggcc ggctcggccg gccccccggc tgcgcgctcg gaatacctgt
     3301 tgaagcgcaa ggcgtcgccg cgcagggtga tgcgccgcca gacctggcgc ttttgcgccg
     3361 gcgtcatcgc cggccagtgc tgcacttcct cgaacgagcg cccgcagccc ttgcagctgt
     3421 cgtcgccctg gctggtggag cagatcgcga tgcagggcgt gtcgggcgtg gtctcgtacc
     3481 aggttttcca ggcggcgtac gcggcgggcg gaaagccccg ctcgtcggcc tcctcctggt
     3541 gacggaaaac cagcagcgcg tacacctcgg ccagcgcgcg cagctcggga gcgagcatca
     3601 ccccatcggg cgagggcttg cgggtgcgcc agaagttgat ggcggcctcg atgtcgatga
     3661 tgtggatgcc ggccatagga caaggagctg ggggagggca tgatagcgcc ccaagcggag
     3721 ccgggacgac gccggccccc ggggcggggg cgcctggccc gatggagccg gtgtatgctt
     3781 gccgcccatg atcaagctcc ttgcgaaatg gctgctcagc gccgccgcgc tgctgctcgt
     3841 cgcctatttg tacaccggtg tcgaggtaaa aagcttcccc gcggcactga tcgcggcatt
     3901 catcatcggc cttctgaaca gcgtggtgcg ccccatcctg gtggtcctga cgttgccggt
     3961 cacgatcgtg acattggggc tgttcctttt catcatcaac gcgctgatgt tctgggccgc
     4021 ggccggcgtg ctggacggct tccatgtgac gggtttcctg gccgcgctga tcggctcgct
     4081 gatctactcg ctgataggcg tcgtcatcga gtcagcgctc gagagcctgt tcgcgcagaa
     4141 gtgactcaac ctgccgcgcg cgggtgtcga tgatcgaagc ctcgatgtgg tcgccgcccc
     4201 cggcggcggc cttgcgcgcg agttcctgag cggccttgta gcggtccatc gcggcctggt
     4261 agtccaggtg cgccacctga acttcggctt ccgcgcggat tgcccgcaac gtctgcccct
     4321 gggccgtgta cgcggcgccc agcagctgcc aggcctgggc gtcccgcgga tgcaatgcca
     4381 cccaggtttg aaggcgctgc gccgcctccg ccgcgcggcc gctgttgacg cgggcctggg
     4441 aggacagcag gacttcgggc cgggcgccgg cagcgttgtc cagggcgacc gccggcgtgg
     4501 agtcaccggc ctggagcgcg acttccgccg cgagcaaacc cgcgagccgc gccgcccgcg
     4561 catcgccggc cgtcaagcca gccagttttc cggccagcct ggcggcagcg gccggatcgc
     4621 gcagcttgga gctcgccagg gccgcggcat acacaatgcc aacctgcctc gcccgcgacg
     4681 cggtcgcctg ggcgccattc gcctcggtga cgtagctgcg caggccgtcg acgccggggt
     4741 tggaaaggac ccgggcgcgc gcgaccatca tggcgtgcac cagatcgggc actgaggcag
     4801 ccggccgccc ggcgagctgc tggcgcgact gcatatcggc catgcgttcg gtggtcatcg
     4861 ggtgggtgcg caggtagggg aaggcgccgt tgtcgttcag gcgcgccgcc tgctgcagct
     4921 tgtcgaacat ggtgacgaag ccctgcggct cgaaccctgc ctgggtcagc acgccataac
     4981 cgatgcggtc cgcttcgcgc tccatgtcgc gcgaaaaatt gagctggttc tggatcgcca
     5041 gcgcctggcc cccactcacc agggcattgg ctgcgtcggg gctcttgctg gccgccagcg
     5101 cgcccaggat catggcgccg atcacccacg gggcctggcg gttctgctgc gtcaccaggc
     5161 gcgagatatg gcgctgggtc acgtggctga gttcgtgggc cagcaccgat gccagttcgt
     5221 cgcgcgaggc ggtcacgttg atgaggccca actgcaggcc cagccagccg cccggaagcg
     5281 cgaacgcatt gatgatgcgg tccttgccga gcagcacctg ccacgcaaac cgctcgtcga
     5341 gctccgcggt gagctcgccg cgagctcgcg ccgcggcgag cagccgcttc cagatctcct
     5401 gcacatattc gtcgagcacc gggtcgtcga tgtagtcggg atcgcggtac agctcacgcg
     5461 cgatgcgttc gcccagcttg cgctcggaac tcgcgttcat ctcgctggtg tcccccagcg
     5521 tgggcaactg cgcccgggcg ggcgcggcca gcaaagtgca gctcaatgcc agcaccgccg
     5581 cggatcgcca tcctggccgc gacccggacc cgagccgttt tctccggttg atcatggccg
     5641 tatgatgctc gaagaatatg aacacggcgt aaatggtttt tccccgtgct ttccaggcat
     5701 agcatgagtt cgttgacaca tttcgacgca cagggccaag cccacatggt agacgttggc
     5761 ggcaagaccc ccagccggcg cgtggctgtc gcctcgggcc gtatcgaaat gctgccggcg
     5821 acgctcgcgg ttatcgagtc ggggaccgcc aagaagggcg acgtcctggg cgttgcgcgc
     5881 attgcgggca tccaggcggc caagaagacc agcgagctga ttccgctgtg ccatccgctg
     5941 gcgctgacgc gcttggcgat cgacttcctg atcgagagag cgcctcccgg cggcggcccc
     6001 gcgatcgcct gcacggccac ggccgaaacc gtgggtccca ccggagtgga gatggaagcc
     6061 ctcaccgcag tccaggtcgc cctgctcacc atctacgaca tgtgcaaggc ggtggaccgc
     6121 ggcatggtga tcaccggcgt gcgcctgctg gaaaagcagg gcggcaagtc ggggcactac
     6181 gtcgcgggca agtaaagctg gaaaaggcag ggccgcaaat cggcaagctt cgtcaaccgc
     6241 cggcgtgggt ggggtgagcg accgacgccg gggccggcgc cgcccgctgt gtagcgctcc
     6301 agaccgcata ttcgttcggg aacgccgccc gaaagcggct caggtgtgcc agcgcttcgt
     6361 tgtcccggcc cagcatggcc gcgctctcga tcactttttc gacgacgcgg ggctctggag
     6421 aatagtgcag cagcgcaagc gcgatatcga aagtccattg ggcgttatcg cgcgtcaagg
     6481 gcgtgatcgt cagctccgcg aagcgcacat gatcctggaa caatcgagaa ccccggatgt
     6541 gcgccagggt atcgtcccgg taatcggcgt cccgggcctg cggcggcagg tagatctggc
     6601 tgatgcggcg gtagtcccag gcggcaaagg cgattgccgc gatcagcgcc agcgtcagcg
     6661 cccgctggaa gcccggcgcc caccacccat gacctgaagc gttgctggat gcgcttggcg
     6721 aggaaggccg cagcagcccc aggctcaaga cgaaagcgag ctggaacggg ccgtaccaca
     6781 gagggtactc gagcatgctg tgcaacagga tcaccgccag cacgctccag gccagttgcc
     6841 gcgtgcgatc cgtttcgcgc cacggttgcg cgcgaacgac ccaccacagg aaagcgccgc
     6901 cgatgatggc agccgcgggg acacccagct ccacggccag gtgcagcggc aggttgtggg
     6961 cattgtcgag aatggcgcaa aaccgcgccc ccggatacga agtgatgtag tgcgcatagt
     7021 ccagttcgcc ccacccccag cccagccagg gtcgcgcgga gatgaggtcc agcacgttcg
     7081 accagaggac aacgcggctg ctgcagcctt cgccccgcgt catccggccc cagatggtgt
     7141 cgggtgccat cccggtaatg gcctccagca acacaggcaa tagcgttgcc gccagcagat
     7201 aggccagcgc tgcggccaga cacaattcaa gtcgacggcg tcgatcgggc ccggaccaca
     7261 ggcccgtgaa aagggccagc atcaccacct ggagcagccc cgtgcgcgaa gctgaggcgg
     7321 cgttggccgc agccagcaac gccattgccg cgacagcgtg gccgcgcctc gtgcctggcg
     7381 cccaccatag caaggccgcc atgccgatgg cggtcaggga ggcgaattgg ttgcgctggc
     7441 gcagattggc aaaagcttcg ccgacgccgg tggcgtttgc gagcggtgaa agataggaag
     7501 ccagcccgaa gtactggaac agcgcaatcg cgctgctgat cagggcggcc gcgatccagc
     7561 cggccgcgat gcactgaacc caatgaagtg gctggaccgc acgcgcttga ctcagccggt
     7621 acgcggccca ggcga
//
LOCUS       DASWIY010000124         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_68197, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000124 DASWIY010000000
VERSION     DASWIY010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68197"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VGA59_04770"
     CDS             complement(<1..1007)
                     /locus_tag="VGA59_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9719023.1"
                     /translation="MNFLWPEFLWLLLALPLLVLLYVWLLRRKKKLALRYASLSIVKE
                     AMGAGQTMRRHIPPLLFLLALAAMLLATARPHAVVTLPSNQQTIILAIDVSGSMRATD
                     VQPNRLVAAQNAAKSFLAELPRHVKVGIVAFAGSAQIAQLPTTNREDLVTAIDRFQLQ
                     RATATGNAIVISLATLFPDAGIDLQSLQMGRERQRGIAIDGDKKEKMEFTPVAPGSYS
                     SAAIIMLTDGQRTTGVDPMEAAKIAADRGVRVYTVGIGTVDGETIGFEGWSMRVRLDE
                     ETLKAIANKTAADYFYAGTATDLKKVYETLSSRLTVEKKETEISALFAMAAAALALLS
                     AG"
     gene            complement(1027..2043)
                     /locus_tag="VGA59_04775"
     CDS             complement(1027..2043)
                     /locus_tag="VGA59_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9719024.1"
                     /translation="MKSSQPNASPTQNAATRAASGGAAQGAAGGPRENAEHVLRRLEW
                     TVIRRLDGLLQGDYRTLLRGAGLDLADLREYQHHDDVRHIDWNVTARLQVPHVRVFTE
                     DREMAAWFLLDLSPSVDFGSGEQRKRNVSAEFVAVIARLLTRHGNRVGALLYGSGVDT
                     MIPTRGGRRHVLHLLHSIQSRPVAVESGTTKLRELLESAAGTIKRRSTLFVVSDFISE
                     PGWERPLALLAQRHEVVAVRLLDPLELDLPDLGLLTMRDSETGEQILVDTHDKGFRKR
                     FARIAAQREAELRQAFVRAAVDALELSTDGELVDAVVRFADMRKRRARLASGSMPAHL
                     KRTA"
     gene            complement(2024..3031)
                     /locus_tag="VGA59_04780"
     CDS             complement(2024..3031)
                     /locus_tag="VGA59_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9719025.1"
                     /translation="MDQTARQDSATAQLMEQILYEVKRVVVGQDRFLERVMVAMLAQG
                     HLLVEGVPGLAKTLTVKTLANTIHGRFKRIQFTPDLVPADLVGTRIYNQKTGEFGTSL
                     GPVFTNLLLADEINRAPAKVQSALLEVMQERQVTIAGETHKVPNPFLVMATQNPIETE
                     GTYPLPEAQVDRFMMKVLVDYPTDEEEFVIVERVTGPAVNVAAVATTEQLAALQDECR
                     RIYVDPSLVQYAVKLVSATRTPERHGLKDLGKYITFGASPRATINMTEGARALAMLRG
                     RTYALPEDMTDLVPDVLRHRLVLSYEALSEGLSPDALVGKIMTKIPAPPKPLEHEKLA
                     A"
BASE COUNT          477 a         1020 c         1034 g          536 t
ORIGIN      
        1 cccgccgaca gcaaggccag cgccgccgcg gccatggcga acagcgccga gatttccgtt
       61 tccttctttt ccaccgtcag gcgtgagctc agcgtttcat agaccttctt gaggtcggtc
      121 gccgtgccgg cgtagaagta atccgcggcg gtcttgttgg caatggcctt gagggtttcc
      181 tcgtccaggc gcacgcgcat cgaccagccc tcgaatccta tggtttcccc gtccacggtg
      241 ccgataccca ccgtgtacac gcgcaccccg cggtcggccg cgatcttggc ggcttccatc
      301 gggtccaccc cggtggtgcg ctggccatcg gtcagcatga tgatcgcggc ggaagagtag
      361 gagcccggcg cgaccggcgt gaattccatc ttctccttct tgtcgccgtc gatcgcaatg
      421 ccacgctggc gttcgcgccc catctggagc gactgcaggt cgatgccggc atcggggaac
      481 agcgtggcca gcgagatcac gatggcgttg ccggtggccg tggcgcgctg gagctggaag
      541 cggtcgatcg cggtgaccag gtcttcgcga ttcgtcgtgg gtagctgcgc aatctgcgcc
      601 gaacccgcga aggcgacgat gccgaccttg acgtgacgcg gcagttcagc caggaacgac
      661 ttggcggcgt tctgcgcggc caccagccgg ttgggctgca cgtcggtggc gcgcatgctg
      721 cccgacacat ctatcgccag gatgatggtc tgctggttgg acggcagcgt aaccaccgca
      781 tggggccgcg ccgtcgccag cagcatcgcc gccagggcca gcaggaagag caggggcggg
      841 atgtggcgtc gcatcgtctg gccggcgccc atggcctcct tgacgatgga cagcgacgca
      901 tagcgcagcg ccagcttttt cttgcgccgc agcagccaga catagagcag cacgagcagc
      961 ggcagcgcca gcagcaacca gagaaattcg ggccagagga aattcatgga agtcgtcctc
     1021 tttacttcaa gcggtgcgct tcaggtgcgc aggcatgctg ccggaggcaa gccttgcgcg
     1081 gcgcttgcgc atgtcggcga agcgcaccac ggcatctacc aattccccgt cggtggacag
     1141 ctccagcgcg tcgacggccg cgcgcacgaa ggcctggcgc agttcggcct cccgctgggc
     1201 ggcaatacgc gcgaagcgct tgcgaaagcc cttgtcatgg gtgtccacca gaatctgctc
     1261 gccggtctcg gagtcgcgca tggtcagcag ccccaggtcg ggcaggtcca gctccagcgg
     1321 gtccagcagg cgcacggcca ccacctcatg gcgctgggcc agcagcgcca gcggccgttc
     1381 ccagccgggt tcgctgatga agtcggagac cacgaacagc gtggagcggc gcttgatcgt
     1441 gccggcggcc gattcgagca gttcgcgcaa cttcgtcgtg cccgactcca cggcgaccgg
     1501 ccgcgactgg atgctgtgca gcaggtgcag cacatggcgc cgcccgccgc gcgtggggat
     1561 catcgtgtcc acgccagacc cgtagagcag ggcgccgacg cggttgccgt gccgcgtgag
     1621 cagccgcgcg atgacggcga cgaactcggc cgacacgttg cgcttgcgct gctcgcccga
     1681 tccaaagtcg accgacgggc tcaggtccag caggaaccag gccgccatct cgcggtcctc
     1741 ggtgaacact cgcacatggg gcacctgcag ccgcgccgtg acgttccagt cgatatggcg
     1801 cacgtcgtcg tgatgctggt attcgcgcag gtccgccagg tccaggcccg cgccgcgcaa
     1861 cagggtgcgg tagtcgccct gcagcaagcc gtcgagccgg cggatgacag tccattcgag
     1921 gcggcgcagc acgtgttcgg cgttctcacg cggccccccc gcggcgcctt gcgccgcgcc
     1981 accggacgcg gccctcgtgg ccgcgttttg tgtcggcgaa gcgttaggct gcgagctttt
     2041 catgttccag cggtttcggc ggcgccggga tcttggtcat gatcttgccg accagtgcgt
     2101 cgggagacag cccttcggac agcgcctcgt acgacagcac cagacgatgc cgcagcacat
     2161 cgggcaccag gtcggtcatg tcctcgggca gtgcataggt gcggccgcgc agcatggcca
     2221 gcgcgcgcgc gccctcggtc atgttgatcg tggcgcgcgg gctggcgccg aaggtgatgt
     2281 acttgcccag gtccttgagc ccgtgccttt ccggcgtgcg ggtggcggag accagcttga
     2341 cggcatattg caccagcgaa ggatcgacgt agatgcgccg gcattcatcc tgcagcgccg
     2401 ccagctgctc ggtcgtcgcg accgccgcca cgttcacggc cgggccggtg acgcgctcga
     2461 cgatgacgaa ctcttcctcg tcggtcgggt agtccaccag caccttcatc atgaagcggt
     2521 ccacctgcgc ctcgggcagc gggtaggttc cctcggtctc gatcgggttc tgcgtggcca
     2581 tcaccaggaa cggattgggc accttgtgcg tttcgcccgc gatggtgacc tgccgctcct
     2641 gcatcacctc cagcagcgcg ctctgcacct tggccggggc gcggttgatt tcatcggcca
     2701 gcagcaaatt ggtgaacacc gggcccagcg acgtgccgaa ttcgccggtc ttctggttgt
     2761 agatgcgcgt gcccaccagg tcggccggca ccaggtcggg cgtgaactgg atgcgcttga
     2821 accggccgtg gatggtgttg gccagcgtct tcaccgtgag cgtcttggcc aggccgggca
     2881 cgccttcgac cagcaggtgc ccctgcgcca gcatggccac catcacgcgc tccaggaagc
     2941 ggtcctggcc gaccacgacc cgcttgacct cgtagaggat ctgttccatc aattgggcgg
     3001 tggcgctgtc ctggcgggcg gtttggtcca tgggttgtgg gctcatcgtt ggtggcaaaa
     3061 gcaaaag
//
LOCUS       DASWIY010000125         9867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1556351, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000125 DASWIY010000000
VERSION     DASWIY010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556351"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1061..1516
                     /gene="rplM"
                     /locus_tag="VGA59_04785"
     CDS             1061..1516
                     /gene="rplM"
                     /locus_tag="VGA59_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX9719026.1"
                     /translation="MTTFSAKPADVTHEWFVIDATDKVLGRVASEVALRLRGKHKAIY
                     TPHVDTGDFIVIINAAQLRVTGTKNQDKMYYRHSGYPGGISATNFRDMQAKHPGRALE
                     KAVKGMLPKGPLGYAMIKKLKVYGGAEHPHTAQQPKLLELPDAELGARK"
     gene            1513..1905
                     /gene="rpsI"
                     /locus_tag="VGA59_04790"
     CDS             1513..1905
                     /gene="rpsI"
                     /locus_tag="VGA59_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX9719027.1"
                     /translation="MIGDWNNGTGRRKSSVARVFLKKGTGKITINGRDIQEFFGRETS
                     IMICKQPLFLTNHVQTFDIQVNVHGGGESGQAGATRHGITRALIDYDAALKPALSQAG
                     FVTRDAREVERKKVGLHSARRRKQFSKR"
     gene            1999..2706
                     /locus_tag="VGA59_04795"
     CDS             1999..2706
                     /locus_tag="VGA59_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEX9719028.1"
                     /translation="MRFRLLRRRLTISAPRMAVRSAMPWPLRWAGAAIVLGFCAAISL
                     WAFEFGKSIAGLDTDAKQELVRLRADAAKLREERDKAQSVLNTSASLITAEKTAQERL
                     AMQIKALEVENRALRDDLGFFEKLIPTSGAEGLAIRALQAEVLGGTQLKWQVLVIQPV
                     KNAQEFRGKLEVSIGGTLNGRPWMMPLPGGAQPLQFRQYRRIEGMVDLPPAAVVKNVS
                     AKVVEGTATRAVQSIKL"
     gene            2817..3212
                     /locus_tag="VGA59_04800"
     CDS             2817..3212
                     /locus_tag="VGA59_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX9719029.1"
                     /translation="MFGKKAQPPIKSLIAQGSRIEGNLKFTEGLRVDGEVCGNIQANS
                     EQPSILVISEAAVVQGEVSADHVIINGTVRGPVQAKELLELQPKARIEGDVCYKALEM
                     HQGATISGLLRPLLGADEKPLLKLAANNP"
     gene            3272..3637
                     /gene="erpA"
                     /locus_tag="VGA59_04805"
     CDS             3272..3637
                     /gene="erpA"
                     /locus_tag="VGA59_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX9719030.1"
                     /translation="MSAVAEQVQTEMPAPLIFTDSAAAKVADLIAEEGNPDLKLRVFV
                     QGGGCSGFQYGFTFDEITNDDDTVMTKNGVSLLIDAMSYQYLVGAEIDYKEDLQGAQF
                     VIKNPNAGSTCGCGSSFSA"
     gene            complement(3742..4851)
                     /locus_tag="VGA59_04810"
     CDS             complement(3742..4851)
                     /locus_tag="VGA59_04810"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX9719031.1"
                     /translation="MPDYYIGLMSGTSLDGVDGVLADFPAAGPVVIGHASAPFPAGLR
                     SELLQLNAPGPDELHRAALAGNALMRVYAAVVGELLEKAGVAASAIRAIGAHGQTVRH
                     RPQEFDGTGYTLQLCQPALLAELTGIDVAADFRSRDVAAGGQGAPLAPFFHQELFARP
                     GETVGVLNIGGISNLTLLRADGSMLGFDCGPGNCLIDAWCARHTGRPYDAEGAWAATG
                     NVLPPLLALLLAEPYFPKAPPKSTGRDLFSQAWLERHLRAFGGAAPADVQSTLTELTA
                     RACSDDVARHESALRRLVVCGGGVFNRHLMQRLRALLPQVQVSSSADWGLPPLQVEAT
                     AFAWLARRAVRREKLELQSITGARGARVLGCVYPA"
     gene            complement(4899..6257)
                     /locus_tag="VGA59_04815"
     CDS             complement(4899..6257)
                     /locus_tag="VGA59_04815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9719032.1"
                     /translation="MNNGLIAAGEQLLSRAAHALEHHPKHVTAVIAALMLGGGGGAFA
                     VASLGPDPSTMPVRHVLQAVETLPLQQQAQELDLHGLKLFRSDVARATDTVEGLLARL
                     GIDDPAAAAYLRKDATFRTQLLGRAGRSVTVEASAGHALEKLSVRWAPQDDGTFNRLV
                     IERTPEGGFTSRVEIAPLVAAARMGSATVRTSLFGAADEARIPDAVVTQVVQIFSGDI
                     DFHNALRVGDRLSVVYEALEADGEPMRTGRILSVEFVNNGKRHQAVWFEEPGSKGTYY
                     TLDGKSLDTSYLASPMAFSRVTSGFAMRFHPVLLRWKAHRGTDYGAATGTPVRTVGDG
                     VVAFAGKQNGFGNVIVVKHNNTDETLYAHLSRIDVRPGQNVSQGQNIGAVGMTGWATG
                     PHLHFEFRVNGVHKDPLAIARHGNAVPLSAAARPAFDRLAQSMRSQLAVASSMQVVAS
                     AE"
     gene            6438..7670
                     /gene="tyrS"
                     /locus_tag="VGA59_04820"
     CDS             6438..7670
                     /gene="tyrS"
                     /locus_tag="VGA59_04820"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX9719033.1"
                     /translation="MNQASVTTFPVTDRVREALAVSLRGSQELIPQEEWLQKLAKSEA
                     TKDPLRIKLGLDPTAPDIHVGHTVVLNKMRQLQDLGHTVIFLIGDFTSMIGDPSGRNT
                     TRPPLTADQIKANAETYYRQASLVLDPAKTEIRYNSEWSDPLGARGMIQLAAKYTVAR
                     MMERNDFHERFKAGSSISVHEFLYPLMQGYDSVALKSDLELGGTDQKFNLLMGRHLQQ
                     EYGQEPQCILTMPLLEGLDGVEKMSKSKNNYIGINEDANTMFAKVLSISDDLMWRWYT
                     LLSFRSEAGIAELKKEVEAGRNPKDAKVLLAKEITARFHSAQAADAAEQDFVNRSKGG
                     VPDEIPGVQLTGAPLGIGTLLKQAGLAPSTSEANRLIDGGGVRVDSSVVTDKGLKLEA
                     GTYVVQVGKRKFAKVMLA"
     gene            7713..8006
                     /locus_tag="VGA59_04825"
     CDS             7713..8006
                     /locus_tag="VGA59_04825"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX9719034.1"
                     /translation="MITNIDIDEKLVAQAMSVSGARTKREVVDRALREMVARAKRPRL
                     RDVFGRATEDTYWPDYDPKTSPGEPPGKYRVEQGVATYKVAPSSPVAKPRRKR"
     gene            8006..8413
                     /locus_tag="VGA59_04830"
     CDS             8006..8413
                     /locus_tag="VGA59_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HEX9719035.1"
                     /translation="MLLIDSSVWIDSHRGRVTDATRFVDTRDDHEELATTGIIFQEVL
                     QGVRDEAEFDYLREILWSALILEPRELSTYEVAAHLYLRARAKGFTIRKPNDCLIAAL
                     ALEHGALLVHNDRDFLALAQVEPALLIYPGRPH"
     gene            8445..9362
                     /locus_tag="VGA59_04835"
     CDS             8445..9362
                     /locus_tag="VGA59_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9719036.1"
                     /translation="MTLASSAKALPPPLLLRMSIAVAVATIALKTLAWWITDSVGLLS
                     DAMESLVNLASAMFALLMVTIAHRPADADHPYGHHKAEYFSSGFEGLLIIVAALGIVW
                     AAVQRLLNPQPLDQLGWGLGLSVISSALNGALAWAMLQSGRQHRSIALEGDARHLITD
                     VWTSAGVVAGVGLVALTNWQWLDPAVAIGVALNILREGAALMWKSSQGLMDEALEPEV
                     MADIEKTLAGFDHHTIRFDHISSRRSGRRRFVDLHMHLPASWSLGRAAALRASVEQAL
                     MSEVPGLRATIQLLPSDVEAHFEDEKDLI"
     gene            9359..9799
                     /gene="dtd"
                     /locus_tag="VGA59_04840"
     CDS             9359..9799
                     /gene="dtd"
                     /locus_tag="VGA59_04840"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX9719037.1"
                     /translation="MIALIQRVSQARAVIGDLTAGEIGAGLLVLVCAERGDSEVQADK
                     LLVKLLKLRIFADEAGKMNRSVQDIGGGLLLVSQFTLAADTSGGNRPSFTNAAAPEEG
                     RRLYEYFVAQARAAHPVVQTGEFAAHMQVHLVNDGPVTIPLRVS"
BASE COUNT         1880 a         3121 c         3213 g         1653 t
ORIGIN      
        1 gacgggccgg gcggggcagt gccggtgccg ggcgcgggct cggcggcagg agtaggcgac
       61 gccagcgtgg gagtcggagt cggagtcgga gtcggagtcg gagtcggagt cggagtcggg
      121 gtcggggtcg gcgtcggggt cggcgtcggg gtcggggtcg gggtcggggt cggcgtcggg
      181 gtcggggtcg gcgtcggggt cggagtagga gtaggagtag gagtaggagt aggagtagga
      241 gtcagagcag gaacttgacc cccggaagca ccggatccca gagccgtgat ctgtccgcga
      301 atttcgccgg cggggagcgc tgcgctatgg acattgaagt aatagccgcc ggacttgagc
      361 aaatcgactt gcgcgtcggt aagcggcgcc gtcgtcgtcg tccaaatgcc gcttccagca
      421 gccgtttgcg tcaatggaaa tacaattggt ccgctgattc cgacagcacc gctgtgaatg
      481 tgcgcagctg tgccgacgac gccggtggtc gtgacgtttg ccgtgagaat attggttgca
      541 gaatcgaaat tcgccgtacc gctgccggta gcagtactcg cgttgggcgg aacttcttgc
      601 acgccggtaa gggtggccac gaattcgcga acctcgtcat cgcccccacc gcaagacact
      661 aacgcggcga gcaggattac cgatagcagc caacccagca ccgattttgc attaagtgat
      721 ctcatgagaa tgtctccagc taagttgaac caacatgaaa ctcccttgag cctggggttc
      781 tatatgagaa gagcgcctgc tggcaagtgg gccgatttcc cttattgcgc catggtttct
      841 tcgtgaaaat cggcttgcgc gctggcggcg atcttggcta tttccgagcg ctgctcggag
      901 gtcatattgc gccaagtcct tgatttttcg tataatcttt ggctccgcag cgcattgtgg
      961 ccccgcgacg gagcttgaac cccacctaag agattcccgc cagaggaaca ccgaccgtgg
     1021 aaaagggccc tccaaaccac tttttcgaaa gaaaagactc atgacaacgt tcagcgcaaa
     1081 acccgctgac gtgacgcacg agtggtttgt gattgacgcc accgataagg tgctcggacg
     1141 agtagccagc gaagttgctc tccgtttgcg cggcaaacac aaggccattt acacgcctca
     1201 cgtcgatacc ggtgacttca tcgtcatcat caacgcagcc cagttacgcg tgacgggcac
     1261 caagaaccag gacaagatgt actaccgcca ctcgggctac ccgggcggaa tcagtgccac
     1321 caacttccgc gacatgcaag ccaagcatcc ggggcgcgcg ctggaaaagg ccgtcaaggg
     1381 catgctgccc aagggcccgc tcggctacgc gatgatcaag aaactcaagg tgtacggcgg
     1441 tgccgagcat ccgcacaccg cgcagcagcc caagctgctc gaacttcccg acgctgaact
     1501 gggggcgaga aaatgattgg tgattggaac aatggtaccg gccgtcgcaa gtccagcgtc
     1561 gcccgtgtgt ttctgaaaaa gggcacgggc aagatcacga tcaacggccg ggacatccag
     1621 gagttcttcg gccgcgagac ctcgatcatg atctgcaagc aacccctgtt tctgacgaac
     1681 cacgtccaga cgttcgacat ccaggtcaac gtgcacggcg gcggtgagtc gggccaggcc
     1741 ggcgccaccc gccacggcat cacgcgcgcg ctgatcgact acgacgcggc cctcaagccc
     1801 gcgctgagcc aggcgggctt cgtgacgcgc gatgcgcgtg aagtcgaacg caagaaggtc
     1861 ggcctgcact ccgcacgccg gcgcaagcag ttcagcaagc gctgaccgac ctgttaccgc
     1921 cgaaacaaag ccgcacaagt ttcttgtcgc ggctttttta tcggctgtat tgttgagctg
     1981 aggagacttt ccgccgagat gcgattccga ctgcttcgac gccgcctgac gatcagcgcc
     2041 ccgcgcatgg ccgtgcgcag cgccatgccc tggccactgc gctgggccgg cgccgccatc
     2101 gtgctggggt tttgcgccgc catcagcctg tgggccttcg aattcggcaa gagcattgcc
     2161 ggcctggaca ccgacgccaa gcaggaactg gtgcgcctgc gagccgacgc ggccaagctg
     2221 cgcgaggagc gcgacaaggc ccagtcggta ctcaacacgt ccgccagcct gatcacggcc
     2281 gagaagacgg cacaggaacg cctcgccatg cagatcaagg cgctggaagt cgagaaccgc
     2341 gcgctgcgcg acgacctggg ctttttcgag aaactgatcc ccaccagcgg cgcggaagga
     2401 ctggctattc gcgccctgca ggccgaggtg ctggggggca cgcaactgaa atggcaggtg
     2461 ctggtgatcc agcccgtgaa gaacgcccag gagttccgcg gcaagctcga agtgagcatc
     2521 gggggcacct tgaacggcag gccctggatg atgcccttgc ccgggggcgc tcagccgctg
     2581 cagttccggc agtaccggcg tatcgagggc atggtcgacc tgccccccgc ggccgtggta
     2641 aaaaacgtgt ccgccaaggt cgtggaaggc acggccaccc gtgccgtaca gagcatcaag
     2701 ctgtaaccag agcagcgagc atcgaggaaa gaatatgagc cgccacgatt acattcgttc
     2761 tccgcccccc gagggcaccc atgccctcct gcgggcggct cgtcggaggc acgatcatgt
     2821 tcggtaagaa ggcgcaaccc ccgatcaaga gcctgatcgc gcagggcagc cgcatcgaag
     2881 gcaatctgaa gttcaccgag ggcctgcgcg tcgacggcga ggtgtgcggc aatatccagg
     2941 ccaattcgga gcagcccagc atcctggtca tttccgaggc ggcggtggtg cagggcgagg
     3001 tcagcgcgga tcacgtcatc atcaacggaa cggtgcgcgg gccggtgcaa gccaaggaat
     3061 tgctggaact gcagcccaag gcccggatcg aaggggacgt ctgctacaag gcgcttgaaa
     3121 tgcaccaggg cgccaccatt tccgggctgt tgcgcccgct cctgggcgcc gacgaaaagc
     3181 ccttactcaa gctggcggca aataacccgt gagtgcaatt cccgacccgt ttgcagtact
     3241 attgctctga aaccagctat ccggagaccc catgagcgca gttgccgaac aagtccagac
     3301 cgaaatgccc gcacccctga tcttcacgga cagcgcggcg gccaaggtgg cggatctgat
     3361 cgccgaagaa ggcaatcccg acttgaaact gcgtgtattc gtccagggcg gcggctgctc
     3421 cggcttccag tacggtttca ctttcgacga aatcaccaac gacgacgaca ccgtgatgac
     3481 caagaacggc gtgtcgctgc tgatcgacgc catgagctac caatacctgg tgggtgccga
     3541 gatcgactac aaggaagacc tgcaaggcgc ccagttcgtg atcaagaacc ccaacgcggg
     3601 ctccacctgc ggctgcggtt ccagtttttc agcctgatac tggccggccg ccgcccctgt
     3661 cattgcggcc cgcaatccag gaattcatgg atgcccccgg atcaggtccg gggcaggctt
     3721 gtcaagcccg caatgacaaa tctaggccgg atacacacaa cccagcacac gggcgcccct
     3781 ggcgcccgtg atgctttgca gctccagctt ttcgcgccgc acggcccggc gggccagcca
     3841 ggcaaaggcc gtggcctcta cctgcagcgg cggcaggccc cagtccgcgc tggacgacac
     3901 ctggacctgg ggcaacaagg cccgcagccg ctgcatcagg tggcggttga aaaccccgcc
     3961 gccgcacacg acaaggcggc gcagtgcgct ctcgtgccgc gcgacgtcgt cgctgcaggc
     4021 ccgtgccgtc agctcggtca gggtcgattg cacgtcggcc ggcgcggccc cgccaaacgc
     4081 cctcaaatgc cgctcgagcc aggcctggct gaaaagatcg cgcccggtgc tcttgggcgg
     4141 cgccttgggg aagtagggct cggccagcag caacgccagc agtggcggca gcacgttgcc
     4201 ggtcgcggcc caggcgccct cggcgtcgta gggccggccg gtgtgccgcg cacaccatgc
     4261 gtcgatcaaa cagttgccgg ggccgcagtc gaaacccagc atggagccat cggcgcgcag
     4321 cagcgtgagg ttggatatgc cgccgatgtt gagcacgccc acggtttcac cgggccgcgc
     4381 gaacagctcc tggtggaaga acggcgccag cggcgcgccc tggccgccgg cggctacatc
     4441 gcggctgcgg aagtcggctg ccacgtcgat cccggtgagt tcagcgagca gcgcgggctg
     4501 gcacagttgc agcgtgtagc cggttccgtc gaattcctgc ggccggtgcc gcacggtctg
     4561 gccatgggcg ccgatcgcgc ggatcgccga ggcggccacg ccggcctttt cgagcagctc
     4621 gcccaccacg gcggcataga cgcgcatcag cgcattgccg gccagtgcgg cgcggtgcaa
     4681 ctcatcgggg cccggggcgt tcagctgcag cagctcggag cgcaatccgg ccgggaaagg
     4741 ggcgctcgcg tgtccgatga cgaccggccc ggccgccggg aagtccgcca gtacgccgtc
     4801 gacgccgtcg agcgacgtcc cggacatgag gccgatgtaa tagtcaggca tgcggcgatg
     4861 ttagcgggct tgcctggtgc agccgcgccg cgcgaaactc actcggcgct ggccactacc
     4921 tgcatcgagg acgccacggc gagctgggag cgcatcgact gcgcgagccg gtcgaacgcc
     4981 ggccgggcgg ccgcgctgag cggaaccgcg ttgccgtgcc tggcgatcgc cagcgggtcc
     5041 ttgtgcacgc cattcacgcg gaattcgaag tgcaggtgcg gcccggtcgc ccagccggtc
     5101 atgccgacgg cgccgatgtt ctggccctgg ctcacgttct ggcccggccg cacgtcgatg
     5161 cggctcaaat gcgcatagag ggtctcatcg gtgttgttgt gcttcaccac gatgacgttg
     5221 ccgaaaccgt tctgcttgcc ggcgaaagcg acgacgccgt cgcccaccgt gcgcaccggc
     5281 gtgccggtcg ccgcgccgta gtccgtcccc cggtgcgctt tccagcggag caggaccggg
     5341 tggaaacgca tcgcgaaccc gctcgtcacg cgcgaaaacg ccatcggcga tgccaggtac
     5401 gaggtgtcca ggctcttgcc gtccagcgtg tagtaggtgc ctttgctccc cggctcctcg
     5461 aaccagaccg cctggtggcg cttgccgttg ttcacgaact cgaccgagag gatgcgcccg
     5521 gtgcgcatcg gctctccgtc agcctccagc gcttcgtaaa ccacgctcag gcggtcgccc
     5581 acgcgcagcg cgttatggaa gtcgatgtcg ccggagaaaa tctggacgac ctgggtcacc
     5641 accgcgtccg gaatgcgcgc ttcgtcggcg gcgccgaaca gcgaggtgcg caccgtggcg
     5701 ctgcccatgc gggcggccgc caccagcggc gcgatttcga cgcgtgacgt gaacccgccc
     5761 tccggcgtgc gctcgatcac caggcggttg aaggtgccgt cgtcctgcgg cgcccaccgc
     5821 acgctgagct tttccagtgc atgtccggcg ctggcttcga ccgtgacgga gcggccggcc
     5881 cggcccagca gctgggtgcg gaacgtggcg tccttgcgca gataggcggc agccgcgggg
     5941 tcgtcgatgc ccaggcgcgc cagcagccct tccacggtat cggtggcacg ggctacgtcc
     6001 gagcggaaca gcttgaggcc gtgaaggtcg agttcctgcg cctgctgctg cagcggcagc
     6061 gtctcgacgg cttgcaggac gtgccgcacc ggcattgtgg acggatccgg acccagcgat
     6121 gccacagcga aggcgccgcc gccaccgcca agcatcagcg cggcaatgac tgcggtgacg
     6181 tgtttggggt ggtgttccag cgcatgggct gcgcgagaca aaagttgttc gccggcggcg
     6241 atcaaaccgt tattcaatgg aaatatcctt acagggagaa tctacattgt cctacacgcg
     6301 cgtaggagat cccctacggt tacacactga agccgaaaac actcaggaat ggcgaaaaat
     6361 tcccggaact agaatcggcc acgtgaaaac tggctcaagt atagcgaacg acacccgggc
     6421 caatccagaa accctttatg aatcaagcct ccgttacaac atttcccgta accgatcgtg
     6481 ttcgggaggc gctcgcggtg tcgctgcgcg gttcgcaaga gctgattccg caagaagaat
     6541 ggctccaaaa gctggcgaaa tccgaagcca cgaaggatcc gcttcgcatc aagctgggac
     6601 ttgatcctac agcgcctgac atccatgtcg gacatacagt ggtcctcaac aagatgaggc
     6661 aactgcagga cctcgggcat acggtgattt ttttgatcgg tgacttcacc tcgatgatcg
     6721 gcgacccgtc cgggcgcaat accacccgcc cgccgcttac cgccgatcag atcaaggcca
     6781 acgcggagac ctactaccgg caggccagcc tggtgctgga cccggcgaag accgagattc
     6841 gctacaacag cgagtggagc gacccgctcg gcgcgcgcgg catgatccag ctggcggcga
     6901 agtacacggt ggcgcgcatg atggagcgca acgacttcca cgagcgattc aaggccggca
     6961 gctcgatcag cgtccatgag ttcctgtacc cgctgatgca aggctacgac tcggtcgcct
     7021 tgaagagcga cctggaactc ggcggcaccg accagaagtt caacctgctg atgggccgcc
     7081 acctgcagca ggaatatggc caggagccac agtgcatcct gacgatgccc ctgctggagg
     7141 gcctggacgg cgtcgagaag atgtccaaga gcaagaacaa ctacatcggc atcaatgagg
     7201 acgccaacac catgttcgcc aaggtgctgt cgatctccga cgacctgatg tggcgctggt
     7261 acacgctgtt gagcttcagg agcgaagccg ggattgccga gctgaaaaag gaagtggaag
     7321 ccgggcgcaa tccgaaggac gccaaagtgc tgctggccaa ggagatcacg gcccgcttcc
     7381 acagcgccca ggccgccgat gcggccgagc aggacttcgt gaaccggagc aaaggcggcg
     7441 tcccggacga aatacccggg gttcagttga ccggcgctcc gctgggcatt ggcacgctgc
     7501 tcaagcaggc gggacttgcg ccctcgacca gcgaagccaa tcgcctgatc gacggcggcg
     7561 gtgtgcgcgt ggattcatcg gtggtgaccg acaagggctt gaagctggag gccggcacct
     7621 atgtcgtgca ggtgggaaag cgcaagttcg cgaaggtgat gttggcctga agtcatgttg
     7681 ccgttttgta cagcgaatgt acaatgacgg ccatgatcac caacatcgac atcgacgaaa
     7741 agcttgtcgc gcaggcgatg agcgtttcgg gcgcgcgcac aaagcgcgaa gtggtggacc
     7801 gcgccttgcg ggagatggtg gcccgggcga agcggccgcg gctgcgggat gttttcggcc
     7861 gggcaaccga agacacctac tggccggact acgatccgaa aacttcgccg ggagagccgc
     7921 ccgggaaata tcgcgtggaa cagggtgtcg cgacatacaa ggtggcgccc tcttcaccgg
     7981 ttgcgaagcc gcggcgcaaa cgctgatgct gctgatcgat tcatccgtct ggatcgactc
     8041 gcaccgcggc cgcgtgaccg atgcaacccg gttcgtcgat acgcgagacg atcacgagga
     8101 actcgctacg accggcatca ttttccagga ggtcctccag ggagtacgcg atgaagccga
     8161 attcgactac ctccgcgaaa ttctctggtc cgcactgatt ctcgagccgc gggaactgtc
     8221 cacctatgaa gtcgccgcgc acctctatct gcgcgcgcgg gccaagggct tcaccatccg
     8281 caagcccaat gactgcctca tcgccgcgct tgcccttgaa catggggcgc tgctcgtgca
     8341 caacgaccgg gatttccttg cgctggcgca agtggaaccg gccctgctga tctatcctgg
     8401 ccggccacat tgacggcgca accccaacgc acacaatcca gcccatgacc ctggccagct
     8461 cagcgaaggc gctgccgccc ccattgctgc tgcgcatgtc catcgccgtg gcggtggcca
     8521 ccatcgcgct gaagacgctg gcctggtgga tcaccgattc ggtgggcctg ctgtccgacg
     8581 cgatggagtc gctggtcaac ctggccagtg ccatgttcgc gctgctgatg gtcacgattg
     8641 cgcacaggcc tgccgatgcc gaccatccct atggccatca caaggccgaa tacttttcct
     8701 ccggtttcga aggcctgctg atcatcgtgg ccgcgctggg gattgtctgg gccgctgtcc
     8761 agcgcttgct caatccgcag ccgctggacc agctcggttg ggggctgggg ctgtcggtca
     8821 tcagttcggc cctcaatggc gcgctggcct gggccatgct gcagtcggga cgccagcacc
     8881 gatcgatcgc gctggagggc gacgcgcgcc atctgatcac ggatgtttgg acctccgccg
     8941 gggtggtggc cggcgtgggg ttggtcgcgc tcacgaactg gcaatggctg gatccggccg
     9001 tggccatcgg cgtggcgctg aacatcctgc gcgagggcgc cgccctgatg tggaagtcgt
     9061 cgcagggtct aatggacgag gcgctcgagc ccgaggtgat ggccgacatt gaaaagacgc
     9121 tggcgggctt cgaccatcac accatccgct tcgaccacat ctccagccgc cgttccggcc
     9181 ggcggcgttt tgtcgatctg cacatgcacc tgcccgccag ttggtccctg ggccgtgcgg
     9241 ccgcgctgcg cgcctcggtc gaacaggccc tgatgagcga ggtgcccggc ctgcgagcca
     9301 ccatccagct gctgcccagc gatgtggaag cccatttcga agatgaaaag gacctgatct
     9361 gattgccctg atccagagag tgagccaggc gagggcggtg attggcgact tgacggccgg
     9421 cgagatcggc gccggcctgc tggtgctggt gtgcgccgag cgcggcgaca gcgaagtgca
     9481 ggccgacaag ctgctggtca agctgctcaa gctgcgcatc ttcgccgacg aggccggcaa
     9541 gatgaatcgc agtgtgcagg acatcggcgg cgggctgctg ctggtgagcc agttcacgct
     9601 ggctgcggat acctcgggcg ggaatcgccc cagcttcacc aatgccgccg cgcccgagga
     9661 aggccgacgc ctgtacgagt acttcgttgc gcaggcgcgt gcggctcatc ccgttgtcca
     9721 gacgggcgag ttcgcggcgc atatgcaggt acatcttgtg aacgatgggc cggtgacgat
     9781 tccactacgc gtctcgtgat acttgtcatt gcggactcga tccgcaatcc atggatccca
     9841 ggtcaagcct ggtcaagccc gggatga
//
LOCUS       DASWIY010000126        14395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3039839, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000126 DASWIY010000000
VERSION     DASWIY010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 14395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14395
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3039839"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VGA59_04845"
     CDS             complement(<1..977)
                     /locus_tag="VGA59_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9719038.1"
                     /translation="MRRPALYSSSGSGRSGPSAPPTDLPEPAQPQPKPRARFSLSSPR
                     LLWIAILVLAAALAYSHGMGLQARPQRLTQQDINAAVLKTLETQVMPSEYARAYDNIR
                     PAVVRVMSYVKKSRLKDDSHAAKGSAKPKPLGPAPGSEESAGNDEEVENGVGTGVVII
                     DKGIILTNLHVVSGADRIKVVFSDGLESSASITGIQTENDLAVLQASKIPDDLIAATM
                     RSTSDLGPGDKVLAVGFPFGIGPSASGGIISGMKRAFRSPEGKQEMTNLIQFDAAANP
                     GNSGGPLVTMDGEVVGIVTAILNPTPARTFIGIGFAVPIENAAAAAGLPP"
     gene            1093..1953
                     /locus_tag="VGA59_04850"
     CDS             1093..1953
                     /locus_tag="VGA59_04850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX9719039.1"
                     /translation="MPAWIDTHCHLDAPEFSADTEAVRARAAAAEVVHCVLPAVEVSN
                     FAAVRALAHRHADSYALGIHPLATGRAATDDLDRLDRALADAKGDPRLVAVGEIGLDY
                     FVPGLDAERQELFYREQLSLARRHGLPVILHVRRSADRLLKQLRQAGVGGIAHAFNGS
                     MQQAQEFVRLGFKLGFGGTVTFERALQVRRLAAELPIEALVLETDSPDIPPHWLYRTA
                     EQRAAGRPQARNEPAELPRIAQGVADLRGMSLDELAQATMRNAVQALPKLAVAMDCGS
                     SDDNSRSSPQ"
     gene            2211..2963
                     /locus_tag="VGA59_04855"
     CDS             2211..2963
                     /locus_tag="VGA59_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEX9719040.1"
                     /translation="MKQPKLRLPEVNFSDHGDIRYLHLGTEWVQGSMLLDKPFEIELE
                     YVQRMMSWLLFMAPPSVAKRHAMQLGLGAGALTKFCWRKLRMKTTAIELNPQVIAACR
                     LWFKLPADDARLSVILGDAAEVAGHEHWRGTVDALQVDLYDQEAAAPVLDSVAFYADC
                     RRLLTEDGCMTVNLFGRSSSFSHSLQKIVGVFGSGAVWAFRPTREGNTIVLALRAPQC
                     PDRATLAERAETIETRWGLPARKWMRVFKPVD"
     gene            2973..4760
                     /locus_tag="VGA59_04860"
     CDS             2973..4760
                     /locus_tag="VGA59_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEX9719041.1"
                     /translation="MTAVARPKAARSSHRGPLDWRRLVEWLSQDGVISAEEARRTIAR
                     CSQAESAQHPLVRLAAVAMERAGGGKPLDIETLTQWLAGRTGLEYLRIDPLKVDVGKV
                     ADTMSAGYAEHHRVLPVQVTVTEVVVATAEPFLTDWVDEVERQSRRTVRRMLASPSDI
                     HRYTAEFFALAKSVRAAQKAGGNAGAASFEQLVELGRSNKQLDANDQGVVQVVDWLWN
                     YAFDQRASDIHLEPRREQGVIRFRIDGVLHPVYQMPMGVLNAMTARIKLLGRMDVVEK
                     RRPQDGRIKTRNPRGDEVEMRLSTLPTAFGEKMVMRIFDPDTAVKDLDALGFAQHDSQ
                     RWEALVKRPYGIVLVTGPTGSGKTTTLYSTLKRIATEEVNVSTVEDPIEMIEPAFNQT
                     QVQPQLDFGFAEGLRALMRQDPDIIMVGEIRDLQTAEMAVQAALTGHLVFSTLHTNDA
                     PSAITRLMELGIPPYLINATMLGVLAQRLVRTLCKQCRREDESASREALDAIVKPWKI
                     SGRYKPYQPVGCVDCRMTGFLGRMGLYELLTVSPAFKEKVNQSPGIDALRRQAVADGM
                     RPLRLAGALRVAEGMTTLEEVLTATPPLE"
     gene            4865..5353
                     /locus_tag="VGA59_04865"
     CDS             4865..5353
                     /locus_tag="VGA59_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX9719042.1"
                     /translation="MKIKSQKDFFSGLMFMAVGTAFAWGATNYNIGEGARMGPGYFPL
                     MLGILLAAIGAFTIFEALVVETEDGERVGHIAWKPLSFIIASNVVFGIMLGGLPAIGF
                     PSFGLIVGIYALVFIAALAGDEFKFKEVLVLATVLAIGSYVAFVWLLKLQFPVWPSFI
                     VG"
     gene            5366..6880
                     /locus_tag="VGA59_04870"
     CDS             5366..6880
                     /locus_tag="VGA59_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX9719043.1"
                     /translation="MDLINNLSIGFGVAFTPINLLYSFIGVLLGTVIGVLPGIGPLAT
                     IAMLLPATYALPPISALIMLAGIYYGSQYGGSTTAILVNLPGEASSVVTVLDGYQMAR
                     QGRAGPALAAAGIGSFFAGCVGTLMLAAFAAPLTEVAFKFGPAEYFALMVLGLIGAVV
                     LASGSLIKAVGMILLGLLMGLVGTDVNSGVARYSFDVPELTDGIGIVAVAMGIFGYGE
                     IISNLGKHVDEREVFTSKVTGLLPTKEDFKRMIPAILRGTGLGCALGILPGGGALLSA
                     FAAYTLEKKTKLQPGEVPFGKGNIRGVAAPEAANNAGAQASFIPLLTLGIPPNPVMAL
                     MVGAMTIHNIQPGPQVMTSNPELFWGLIASMWIGNAMLIILNLPLIGVWVKLLTVPYR
                     WLFPGIVLFCAIGVYSTNNNTWDIWMVGIWGVIGYMFIKLGAEPAPLLLGFILGPMME
                     ENLRRAMLLSRGDWSVFVTRPLSASLLAAAALLIVIVALPTVKRKREEAFVEVD"
     gene            7167..8483
                     /locus_tag="VGA59_04875"
     CDS             7167..8483
                     /locus_tag="VGA59_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HEX9719044.1"
                     /translation="MQDKHSLPKAHPQRVSLHNEIHARPPEAMSPPLAISHLVMLSDA
                     AGREASRAHVAALLRERHLPLPDAQSTHIRMDLGPFRIRWELHTEFVTWSFSRPIDAA
                     GFGEREPATAIEVVPREWLDGLPGQCLASLHLWVLPTKPFDSGSLVKHVLNEDLLVAS
                     NVADGHAEVYTDFAIHADGFSRMVLLAGSVAPRRLGRLVQRLLEIDTYRMAALLGLPA
                     AREAAAVLAYAERELAELAGAIRAAGRDEEPLLLDRLTKLAGEVESQYAETHSRFSAS
                     AAYFELVDRRIEDIAESRLSGLQTIGEFMERRLSPARATCEWATRRQDALSQRVSRIS
                     NLLRTRVEIEQQQSSQALLATMNTRQGLQLKLQATVEGLSVAAITYYIVGLVSYLAKG
                     AHAIGWPWSAESTAATAIPVVALAVWWSLRRLHSRVFDDKQGSGAV"
     gene            complement(8557..10926)
                     /locus_tag="VGA59_04880"
     CDS             complement(8557..10926)
                     /locus_tag="VGA59_04880"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9719045.1"
                     /translation="MNKLGKYDIRRTLGKGAMGIVYEGFDPVIERTVAIKTILPSQLN
                     GTEYVSVMARFKREAQAAGRLNHPGIVAIYEYGEDFAQDITDEEATVIAGPSAAPGAQ
                     RVAFIAMEFVKGRELRDFFEKGERFPLHEVVRIMSEMLDALDHAHSQGVVHRDMKPAN
                     LILLENGRVKVADFGIARLEASELTQTGAVLGTPSYMSPEQFMGQPVDGRSDLFSCGV
                     ILYQLLTGEKPFTGESTTTIMYKVLREEPVPPTELNLSLPPALDAVMKKALAKQPAQR
                     FQTGSDFAQALRAAIAEAAAPATGAKDAGGVAAAAHTRTGQDARPAAAIAQAGAMPRQ
                     RVAGLWAAGAAAVAALAAVVYMVLGPSRPETPQIAQGGVAPAAPATAVPAAVPAAAAV
                     TPAAEAGTLVISAVGLVDPQDSRFKGNLAAAQQEARNHAKRQVVEKALALYVDKTSLD
                     GNYALIERKLLSNSGAFIRTVLHEGAADAGKGGLVETQTRAVVSVRDVQKSLNQLSRE
                     ERVEFIRNKGDPRISIRMDIGNADGAALGRERSQLAENVVKDRIKSFGFRVWAAEGDN
                     PGAANARPADFAIRGEIKVKQLSTRLPASGLTITKSALTSWTLKAVEIATGEEVYLST
                     RLPSGKSWASEDQALVDIGKLVGDEFSREFFLQHFNFRTQKTSLTITGLPESSSAMML
                     RELRGMRPVLDAQLVAEPGRFALQLPDGSVPEIVQEAILRPLNTKLGQECFTLAGVAG
                     ADVTVAFAPACATPQMRSRLEAGPPAGSRLPTAGSKVPPKPVMPTRNTA"
     gene            complement(10959..11768)
                     /locus_tag="VGA59_04885"
     CDS             complement(10959..11768)
                     /locus_tag="VGA59_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719046.1"
                     /translation="MLIKKSLRYGALAALLSLCGMPALAQPTFGNPSPTAGSETSQQA
                     NAAADAAAYQEVAYTNRAIRGPALVVIPGDVKSNNASFIQKFGPNNIADFGELELSQA
                     NFAVLERANLGHLLQEISLAYNLGDPQAARKTMQVGKLKTTKWVVKFDILKAEQIADN
                     RKGFDGRAIGGLISMFGGGLGGAAAGHVTGSVQTQEASGVWLIGMRYKIMDANTTEQV
                     AQGYKELKMEVGAAATSVMGVSQSARGGVGLDTMVQRLVQTTVWEIDNRYK"
     gene            complement(11805..12461)
                     /locus_tag="VGA59_04890"
     CDS             complement(11805..12461)
                     /locus_tag="VGA59_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719047.1"
                     /translation="MRTSSAILLACWAIAISAQAQGFKFSNPPPEAAAEANAQEARQD
                     MISDQLATPCRNRIKNRKIMVLIGEERNGQVYATQGNFSRHVASVNKRLLSLGLRTYS
                     PEQIRRQVAQEEVDAYFKNDPDRALSASRRLAAQYVLKGVIASHAVRNPIINVNQVSV
                     NLDFTLSDASGRPVSTASARNESYAGSDVSGMALTLIEERADEVVARLYSDYCRNASR
                     "
     gene            12497..13294
                     /locus_tag="VGA59_04895"
     CDS             12497..13294
                     /locus_tag="VGA59_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9719048.1"
                     /translation="MDLGIGGNWALVCGASKGLGFGCAQALVREGVNVVLVARGAQAL
                     EAAVQQLIADPARPADTAVQHVAADITTAEGRAKVFEHRRDFDIVVTNAGGPPPGDFR
                     SWDREAWIKAVDANMLTPIELIKATVDGMAARGFGRIINITSSAVKAPIDILGLSNGA
                     RSGLTGFVAGVARTSKLASANVTINNLLPGAFDTDRLKATMAGASEKTGKPIEVVMQA
                     RRATIPARRFGTPEEFGAVCAFLCSRHAGYITGQNILTDGGAYPGTY"
     gene            13404..14387
                     /locus_tag="VGA59_04900"
     CDS             13404..14387
                     /locus_tag="VGA59_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX9719049.1"
                     /translation="MSRAVQIAQHGGPEQLKIAEVGVGEPGPGEIRIRHRAVGLNFID
                     VYHRTGLYPLKMPATLGMEASGVVEAVGEGVQHLKAGDRAAYASQPPGSYCELRVMPA
                     KCVCRLPEPIDFDTAAAMMLKGLTAQYLLKRTQPQGGLKQGDFVLFHAAAGGVGLIAC
                     QWARAMGLQLIGTAGSDAKCALAREHGAAHAINYSTEDFLARVKEITGGKGVKVVYDS
                     VGKDTWEKSLDCLQPFGLMACFGNASGPVPPFAPGILAPKGSLYVTRQTLFTHIATRE
                     ATQAMADDLFEAVTSGKVKIRIDQRYPLEQVQQAHRDLEARKTTGSTILTL"
BASE COUNT         2304 a         4707 c         4873 g         2511 t
ORIGIN      
        1 ggcggcaggc ctgccgcggc ggccgcgttc tcgatcggca ccgcgaaacc gatgccgatg
       61 aaggtgcgcg ccggcgtggg gttcaggatc gcggtgacga tgccgaccac ttcgccgtcc
      121 atggtgacca gcggcccgcc cgaattgccg gggttggccg ccgcgtcgaa ctggatcagg
      181 ttggtcattt cctgcttgcc ctcgggcgag cggaatgcgc gcttcatgcc ggagatgatg
      241 ccgccggagg ccgacgggcc gatgccgaac ggaaagccca cggccagcac cttgtcgccc
      301 gggcccaggt cgctggtgga gcgcatggtg gcggcgatga ggtcgtccgg aatcttgctg
      361 gcctgcagca ccgccaggtc gttctcggtc tggatcccgg tgatcgaggc gctggactcc
      421 aggccgtcgg aaaacacgac cttgatgcgg tcggcgccgg aaaccacgtg caggttggtc
      481 aggatgatcc ccttgtcgat gatcaccacg ccggtgccca cgccgttctc gacttcctcg
      541 tcgttccccg cggactcctc gctgccgggc gccggaccca gcggcttggg cttggcgctg
      601 cccttggccg catgggaatc gtccttcagc cggctcttct tcacgtagct catgacgcgc
      661 acgaccgcgg ggcggatgtt gtcgtaggcc ctggcgtatt cggacggcat gacctgggtt
      721 tccagggtct tgagcaccgc cgcgttgatg tcttgctggg tcagcctctg cgggcgtgcc
      781 tgcaacccca tgccgtggct gtaggccagg gcggcggcca gcacgaggat ggcgatccac
      841 aggagtctgg ggctgctcaa cgagaatctg gcccggggct tgggctgggg ctgggcaggc
      901 tcaggcaagt cggtcggggg agcactgggt cccgaccggc ccgagccgct tgagctgtaa
      961 agggctggcc tgcgcattcg ttcacctatc aagactgcgg ggaaaacgaa aaaaacgaga
     1021 aagcgaaaac gcacggtatc acgctttgcg cgaacctgca ctagtgggga gcttcctcgt
     1081 gcatcatggg gcatgcccgc gtggattgat acacattgcc acctcgacgc gccggagttt
     1141 tcggccgaca ccgaagcggt gcgggcccgg gccgcggcag ccgaagtggt gcactgcgtg
     1201 ctgccggcgg tcgaagtttc caacttcgcc gcggtgcgtg cactggccca tcgccacgcg
     1261 gacagctacg cgctgggcat ccatccgctg gccacaggcc gcgccgcgac cgatgacctg
     1321 gatcggctcg atcgggccct ggccgacgcg aagggcgacc cgcggctggt ggcggtcggc
     1381 gagatcgggc tggattattt cgtgcccggc ctggatgcgg agcgccagga acttttttat
     1441 cgcgagcaac tctcgctggc gcgccgtcac ggcctgcccg tgatcctgca tgtgcgccgg
     1501 tcggccgacc gcttgctcaa gcaattgagg caggcgggtg tcggcggcat cgcccacgcc
     1561 ttcaacggca gcatgcagca ggcgcaggag ttcgtccggc tgggcttcaa gctcgggttt
     1621 ggcggcaccg taactttcga gcgcgcgctt caggtgaggc ggctggcagc cgagctgccg
     1681 atcgaagccc tggtgctgga gaccgattcg cccgacatcc cgccgcactg gctgtaccgc
     1741 acggcagagc agcgcgcggc cggccggccc caggcccgca acgagccggc cgaattgccg
     1801 cgcatcgcgc agggggtcgc cgatttgcgc ggaatgagcc tggacgaatt ggcgcaggcg
     1861 acgatgcgta acgcggtaca ggccctgccg aagctggctg tcgcaatgga ttgcgggtca
     1921 agcgatgaca acagccggtc aagtccgcaa tgacaaagaa gggctgtcat cccggactct
     1981 acaaaaatag gctgtcatcc cggactcgat ccgggatcca ggactcctga attcatggat
     2041 tgcggggccg ggccggagcg gctatgcctc ggcacgtgca tagcgtaggc ccggcattgg
     2101 gctcgctgca ctggacagct gcgcgccccg ggcgttccgc gagcccggcc cgcaatgaca
     2161 acagccgggc aagcccgcaa tgacaaaccc cgggacgata atgctctgca gtgaagcagc
     2221 cgaaactccg ccttcccgaa gtcaatttct ccgaccacgg cgacatccgc tacctgcatc
     2281 tggggacgga gtgggtccag ggctcgatgc tcctggacaa gccgttcgag atcgagctcg
     2341 aatacgtgca gcgcatgatg tcctggctgc tgttcatggc gccgccctcg gtcgcgaaaa
     2401 ggcacgccat gcagctgggc ctgggagcgg gcgcgctgac caagttctgc tggcgcaagc
     2461 tgcggatgaa gaccacggcc atcgagctca acccgcaggt gatcgcggcc tgccgcctgt
     2521 ggttcaagct gccggccgat gacgcgcggc tgtcggtgat cctgggcgat gccgccgagg
     2581 tcgctggtca tgagcattgg cgcggcacgg tcgatgccct gcaggtggac ttgtacgacc
     2641 aggaggccgc cgcgccggtg ctggacagcg tggccttcta cgcggattgc cgcaggctgc
     2701 tgaccgagga cggctgcatg acggtgaacc tgttcggccg gtcctccagc ttcagccata
     2761 gcctgcaaaa gatcgtgggc gtattcggat ccggcgcagt ctgggccttc cggccgacgc
     2821 gcgagggcaa caccatcgtg ctggccctgc gtgcgccgca gtgccccgac cgggccacgc
     2881 ttgccgaacg cgccgaaaca attgaaactc gctggggttt gccggctcgc aaatggatgc
     2941 gggtgttcaa gcccgtggac tgatcttttc ttatgaccgc tgtcgccaga cccaaagccg
     3001 cccgaagcag ccaccgcggc ccgctggact ggcgccgcct ggtggagtgg ctcagccagg
     3061 acggcgtgat ctccgccgaa gaggcccggc gcaccatcgc gcgctgttcg caggcggaaa
     3121 gcgcgcagca tccgctggtg cggctggcgg cggtggcgat ggagcgcgcc ggcggcggca
     3181 agccgctgga catcgagaca ctcacccaat ggctggcggg gcgcaccggg ctggagtacc
     3241 tgcgcatcga cccgctcaag gtggacgtgg gcaaggtggc cgacacgatg agcgccggct
     3301 acgccgaaca ccatcgcgtg ctgccggtgc aggtgaccgt caccgaggtg gtggtggcga
     3361 ccgccgagcc cttcctgacc gattgggtcg acgaagtgga gcggcagtcg cgccgcaccg
     3421 tgcgccggat gctggccagc ccctccgaca tccaccgcta cacggcggag ttcttcgcgc
     3481 tggccaagtc ggtgcgggcg gcccagaaag ccggcggcaa tgccggcgcc gccagcttcg
     3541 agcagctggt cgagctgggc aggagcaaca agcagctcga cgccaacgac cagggcgtgg
     3601 tgcaggtggt cgattggctg tggaactacg cgttcgacca gcgcgccagc gacatccacc
     3661 tggagccccg gcgcgagcag ggcgtgatcc gctttcgcat cgacggcgtg ctgcacccgg
     3721 tctaccagat gcccatgggc gtgctcaatg ccatgaccgc ccgcatcaag ctgctcggcc
     3781 gcatggacgt ggtcgagaag cgccggcccc aggacggccg gatcaagacc cgcaaccccc
     3841 gcggcgacga ggtcgagatg cggctgtcga cgctgcccac cgccttcggc gaaaagatgg
     3901 tgatgcgcat cttcgacccc gacaccgcgg tgaaggacct ggacgcgctg ggcttcgcgc
     3961 agcacgactc gcagcgctgg gaggcgctgg tcaagcgccc ctacggcatc gtgctggtca
     4021 ccgggccgac cggctcgggc aagaccacca ccttgtattc cacgctcaag cgcatcgcca
     4081 ccgaggaggt gaacgtgagc acggtggagg acccgatcga aatgatcgag ccggccttca
     4141 accagacgca ggtgcagccg caactggact tcggctttgc cgaagggctg cgggcactga
     4201 tgcggcagga ccccgacatc atcatggtgg gcgaaatccg cgacctgcag accgccgaga
     4261 tggcggtgca ggcggcgctg accggccacc tggtgttttc caccctgcac accaacgacg
     4321 cgccatcggc catcacgcgc ctgatggagc tgggtatacc accgtacctc atcaatgcca
     4381 ccatgctggg cgtgctggcg caacgcctgg tacgcaccct gtgcaagcag tgccgccggg
     4441 aggacgaaag cgcttcgcgc gaggccctgg acgccatcgt caagccctgg aagatcagcg
     4501 gccgctacaa gccctaccag ccggtgggct gcgtcgactg ccggatgacg ggctttctcg
     4561 ggcgcatggg gctctacgag ctgctgacgg tgagcccggc cttcaaggag aaggtcaacc
     4621 agtcgcccgg catcgacgcg ctgcgccggc aggcggtggc cgacggcatg cggccgttgc
     4681 ggctggcggg cgcgctgcgc gtggccgagg gcatgacgac cctggaggaa gtgcttactg
     4741 cgacgcctcc gctcgagtga gcctgccagt ctccttagcg acaggtaggt gaaacccaca
     4801 acggctgcgc tgcctgttcc caccacaatc gtccatcgga agaattcgcc ccgggagaca
     4861 acacgtgaaa atcaagagcc aaaaggattt cttttctggc ctcatgttca tggcggtcgg
     4921 gaccgcgttc gcctggggtg cgacgaacta caacatcggc gagggcgcgc gcatgggccc
     4981 cggctacttc ccgctgatgc tgggcatcct gctcgccgcc attggcgcgt tcaccatatt
     5041 cgaggccttg gtggtggaaa ccgaggatgg cgagcgggtc ggccatatcg cctggaagcc
     5101 gctgtctttc atcattgctt ccaacgtggt gttcgggatc atgctcgggg gcctgcccgc
     5161 aataggcttt ccgtcttttg gtctcatcgt cggcatctat gcgctggtct tcatcgccgc
     5221 gctcgcgggt gacgagttca agttcaagga agtgctggtg ctggccaccg ttcttgcgat
     5281 cggcagctat gtggcattcg tctggctgct caagctccag ttcccggtct ggcccagttt
     5341 catcgtgggc taaaggatca cccaaatgga tttgatcaac aacctctcca tcggcttcgg
     5401 cgtcgctttc acgccgatca acctgctcta cagtttcatc ggcgtgctgc tgggcacggt
     5461 gatcggcgtg ctgcccggca tcggcccgct cgcgaccatt gccatgctgc tgccggccac
     5521 gtatgcgctt ccgcccatct cggcgctgat catgctggcc ggtatctatt acggctcgca
     5581 atacggcggc tccaccaccg ccatcctggt caatctgccg ggcgaggcgt cgtcggtggt
     5641 cacggtgctc gacggctacc agatggcaag gcagggacga gcgggaccgg cgttggcggc
     5701 tgcgggtatc ggctccttct tcgccggctg cgtcggcacg ctgatgctgg cagcctttgc
     5761 cgcacccctg accgaggtcg ccttcaaatt tggcccggcc gaatacttcg cgcttatggt
     5821 gttgggcctg atcggcgccg tggtgctggc ttccggatcg ctgatcaagg ccgtcggcat
     5881 gatactgctg ggtttgttga tgggcctggt cggaacggac gtgaactccg gcgtggcgcg
     5941 gtactcgttc gacgttccgg agttgaccga cggcatcggc atcgttgcgg ttgccatggg
     6001 catcttcggc tatggcgaaa tcatcagcaa tctgggcaag catgtcgacg agcgcgaggt
     6061 gttcacttcg aaggtgaccg gcctgctccc caccaaggag gacttcaagc gcatgatccc
     6121 ggcgatcctg cgcggtaccg ggctgggctg cgctttgggc attctgccgg gcggcggcgc
     6181 gctgttgtcg gcgtttgcgg cctacaccct cgagaagaag accaagctgc agcccggtga
     6241 agtgccgttt ggcaagggca acatccgcgg cgtggcggca cccgaggcgg ccaacaatgc
     6301 cggcgcccag gcttccttca ttccgttgct gacgctgggc atcccgccca accccgtgat
     6361 ggcgctgatg gtgggggcca tgaccatcca caacatccag ccgggcccgc aggtgatgac
     6421 cagcaacccg gaactgttct ggggcctgat cgcttcgatg tggatcggca atgcgatgct
     6481 gatcatcctg aaccttccct tgatcggcgt gtgggtcaag ctgctcaccg tgccttaccg
     6541 gtggctgttc ccgggaatcg tgctgttctg cgccatcggc gtgtactcca ccaacaacaa
     6601 cacctgggac atctggatgg tgggcatctg gggtgtcatc ggctacatgt tcatcaagct
     6661 gggggcggag ccggcgccgc tgctgctggg cttcatcctg gggccgatga tggaagagaa
     6721 cctgcggcgg gcgatgctgc tttcgcgcgg cgactggagc gtgttcgtca cgcgtccgct
     6781 gtcggccagc ctgcttgccg cggcggcgct gctgatcgtc atcgtggcct tgccgacggt
     6841 caagcgcaag cgcgaagagg ccttcgtcga agtggattga cgggggcatc cttgtcattg
     6901 cggaccccgg cccgatccgc aatccatgaa tcctgaagtc atggatcccg ggtcaagccc
     6961 gggatgacag tcctcccctc tttgtcatag tcctcccccc tttgtcattg cggactcgat
     7021 ccgcaatcca tgaatccttg gagtcatgga tcccgggtca agcccgggat gacagtcttc
     7081 ccctctttgt cgcggcccgg gatgacggtc ctgccttttt cctggcgcca cacacctggg
     7141 ggacaatacc gccctcgagg gaaagcatgc aagacaaaca tagcctgccg aaggcgcatc
     7201 cgcagcgtgt gtcgctgcac aacgagatcc acgcgcgccc accggaggcg atgagcccgc
     7261 cgctggcgat ctcgcacctg gtgatgctga gcgacgcagc cgggcgcgag gccagccgcg
     7321 cgcacgtggc cgccctgctg cgcgagcgcc acctgccgct gcccgatgcg caatccaccc
     7381 acatccgcat ggacctgggg ccgtttcgca tccgctggga actgcacacg gagttcgtga
     7441 cctggagctt ctcgcggccc atcgacgctg ccggcttcgg cgagcgcgag ccggccacgg
     7501 cgattgaagt cgtcccgcgg gaatggctgg atggcttgcc cgggcagtgc ctggccagcc
     7561 tgcacctgtg ggtgttgccg acaaagcctt tcgacagtgg ctcgctggtc aagcacgtgc
     7621 tgaacgaaga cctgctggtc gcctccaacg tcgctgacgg ccatgccgag gtctacaccg
     7681 acttcgcgat ccatgccgac ggtttctcgc ggatggtgct gctggccgga agtgtggcgc
     7741 ccaggaggct gggacgcctc gtacagcgac tgctcgagat cgacacctac cgcatggccg
     7801 ccttgctggg actgccggct gcgcgcgaag cggcggccgt gctggcctat gccgaacgcg
     7861 agctggcgga gctggccggc gcgatccgcg cggccggccg cgacgaggag ccgctgctgc
     7921 tggaccggct gaccaagctg gccggcgagg tggagagcca gtacgcggaa acgcattcgc
     7981 ggttttccgc gagcgctgct tacttcgagc tggtcgaccg gcgcatcgag gacatcgccg
     8041 agtcgcggct gtccggcctg caaaccatcg gtgaattcat ggagcggcgc ctttcgcccg
     8101 cgcgcgccac ctgcgaatgg gccacgcgca ggcaggatgc gctgtcgcaa cgggtctcgc
     8161 gcatcagcaa cctgctgcgc acgcgggtgg agatcgagca gcagcaaagc agccaggcgc
     8221 tgctggcgac gatgaacacg cgccaggggc tgcagctcaa actgcaggcc acggtggagg
     8281 gactgtcggt ggcggccatc acctattaca tcgtcgggct ggtgagctac ctggccaagg
     8341 gcgcgcacgc gatcggctgg ccctggagcg ccgagagcac ggccgccacg gccattccgg
     8401 tggtggcgct ggcggtctgg tggtcgctgc ggcgcctgca ctcgcgggta ttcgacgaca
     8461 agcagggttc cggcgcggtt tgagcaagat ttccgaccag ggcctggtgc cggggctggc
     8521 gccggccacg ttcggcgcca ttgcctcatc gccgattcaa gccgtattgc gcgtcggcat
     8581 caccggcttg ggcggcacct tgctccccgc cgtcggcaag cgcgagcccg ccggcgggcc
     8641 ggcctccagc ctggagcgca tctgcggcgt ggcgcaggcc ggcgcgaagg cgaccgtcac
     8701 atccgcgccc gccacgcccg cgagagtgaa gcactcttga cccagcttgg tgttgagcgg
     8761 ccgcaggatc gcttcctgga cgatctccgg gacgctgccg tccggcaatt gcagcgcaaa
     8821 ccgtcccggc tcggccacca gctgcgcatc cagcaccgga cgcatgccgc gcagttcgcg
     8881 caacatcatg gcgctgctgg attcgggcag gcccgtaatg gtcaggctgg tcttttgggt
     8941 gcggaagttg aagtgctgca ggaagaactc gcgcgagaac tcgtcgccga ccagcttgcc
     9001 gatgtcgacc agagcctggt cttcgctggc ccagcttttt cccgagggaa gccgggtgct
     9061 caggtacacc tcttcgccgg tcgcgatctc cacggccttg agggtccagg aagtcagggc
     9121 ggacttggtg atggtcagcc ccgaagccgg cagcctggtg gacaactgct tgaccttgat
     9181 ttcgccgcga atggcgaaat cggccggccg ggcgttggct gccccggggt tgtcgccctc
     9241 agccgcccag acccggaagc cgaaggactt gatccggtcc ttgaccacgt tttcggccag
     9301 ctgggagcgc tcgcggccca gggcggcccc gtcggcattg ccgatgtcca tgcggatcga
     9361 aatcctcggg tcgcccttgt tgcggatgaa ctcgacgcgc tcttccctgg aaagctggtt
     9421 gagcgatttc tgcacgtcgc gcacgctgac cacggcgcgc gtctgcgttt ccaccaggcc
     9481 gcccttgccc gcgtcggccg ctccctcatg cagcaccgtg cggatgaacg cgcccgagtt
     9541 ggatagcagc ttgcgctcga tcagcgcata gttgccgtcc agcgacgtct tgtcgacgta
     9601 cagcgcgagc gccttctcga ccacctggcg cttggcgtgg ttgcgggcct cctgctgggc
     9661 cgccgccagg ttgcctttga agcgtgagtc ctgcggatcg accaggccca cggcgctgat
     9721 gaccagcgtg ccggcctccg ccgccggcgt gacggcggcg gcggcgggca cggccgccgg
     9781 aacggcggtc gcgggtgccg caggggcgac cccaccctgc gcaatctgcg gtgtctcggg
     9841 gcggctcggc ccgagcacca tgtaaacgac ggccgcgagc gcggccaccg cggcggcccc
     9901 cgccgcccaa agcccggcca cgcgttgccg cggcatggcg ccggcctgcg cgattgcggc
     9961 tgccggcctg gcgtcctgcc ccgtgcgggt gtgcgccgcg gctgcgaccc cacccgcatc
    10021 ctttgccccg gtcgccggcg cggcggcctc ggcgatcgcc gcccgcaggg cctgcgcgaa
    10081 gtcgctgccg gtctggaagc gctgggcggg ttgcttggcc agtgctttct tcatgaccgc
    10141 gtccagtgcc ggcggcagcg acaggttcag ctcggtcggc ggcaccggct cctcgcgcag
    10201 caccttgtac atgatggtgg tggtcgattc accggtgaaa ggcttctcgc cggtgagcag
    10261 ctggtagagg atgacgccgc acgaaaacag gtcgctgcgc ccgtccaccg gctggcccat
    10321 gaactgttcg ggcgacatgt acgagggcgt gcccagcacc gcgcccgtct gagtcagctc
    10381 ggacgcctcg aggcgggcga tgccgaagtc cgccactttg acgcggccat tctccagcag
    10441 gatcaggttg gccggcttca tgtcccggtg caccacgcct tgcgaatggg cgtggtccag
    10501 cgcgtccagc atttcgctca tgatgcgcac gacttcgtgc aggggaaaac gctcgccctt
    10561 ctcgaagaag tcgcgcagct cgcgcccctt cacgaactcc atcgcgatga aggccacgcg
    10621 ctgcgcgccc ggcgcggccg atggcccggc gatgaccgtc gcctcctcgt cggtgatgtc
    10681 ctgggcgaag tcctcgccgt attcgtagat cgcgacgatg ccggggtggt tcagccggcc
    10741 ggccgcctgc gcctcgcgct tgaagcgggc catcacgctg acgtactcgg tcccgttgag
    10801 ctgggacggc aggatggtct tgatggcgac cgtgcgttcg atgacgggat cgaacccttc
    10861 gtacacgatg cccatcgcac ccttgcccag ggtgcggcgg atatcgtact tgcccagttt
    10921 gttcatggca tggacctgcg aaagtggcaa tgggccggtc acttgtaccg gttgtcgatt
    10981 tcccagaccg tggtctgcac caggcgctgg accatcgtgt ccagcccgac gccgcccctg
    11041 gccgactgcg acacgcccat cacgctggtg gcggcggcgc ccacttccat cttcagttcc
    11101 ttgtagccct gcgccacctg ctcggtggtg ttggcatcca tgatcttgta gcgcatgccg
    11161 atcagccaca cgccgctggc ttcctgcgtc tggaccgaac cggtcacatg gccggcagcc
    11221 gcgccgccca atccgccgcc gaacatggag atcagcccgc cgatggcccg gccgtcgaag
    11281 cctttcctgt tatccgcgat ctgctcggcc ttgaggatgt cgaacttgac gacccacttc
    11341 gtggtcttga gcttgcccac ctgcatcgtc ttgcgcgccg cctgcgggtc acccaggttg
    11401 taggccagag agatttcctg cagcaggtgg cccaggttgg cgcgttcgag cacggcgaag
    11461 ttggcctgcg acagctcgag ctcgccgaag tcggcaatgt tgttggggcc gaacttctgg
    11521 atgaagctcg cattgttgct cttcacgtcg ccggggatca caaccagcgc cggcccgcgt
    11581 atggccctgt tggtgtaggc cacctcctgg tacgccgccg catccgcggc cgcattggcc
    11641 tgctgcgagg tttcgctgcc cgcggtgggc gaaggattgc cgaaggtggg ttgggccagc
    11701 gccggcatgc cgcacaggga caacagcgcg gcgagcgcgc catagcggag ggacttcttg
    11761 atcaacatga tgcttgctcc tgaatttagt atttgtcgcc cggatcatcg gctggcattg
    11821 cggcagtagt cgctgtacag cctggcgact acctcgtccg ctcgctcttc gatcaaggtc
    11881 agtgccatgc ccgagacgtc gctgccggca tagctctcgt tgcgggcgga cgccgtggag
    11941 acgggcctgc ccgaagcgtc ggagagcgtg aagtccaggt tcacgctgac ctggttcacg
    12001 ttgatgatgg gattgcgcac cgcgtggctg gcaatgacgc ccttcagcac gtactgcgcg
    12061 gccagccggc gcgaggcgct gagcgcacgg tcagggtcgt tcttgaagta ggcgtcgacc
    12121 tcttcctgag ccacctgcct gcggatctgc tcgggcgaat aggttctcag gcccaggctg
    12181 agcagccgct tgttgaccga tgcgacgtgg cggctgaaat tgccctgggt ggcatacacc
    12241 tggccgtttc tctcctcgcc gatgagcacc atgatcttgc ggttcttgat gcggttgcga
    12301 cagggcgtgg ccagctgatc ggagatcatg tcctggcgtg cctcctgcgc gttggcttcg
    12361 gctgccgctt cggggggcgg attcgaaaac ttgaagccct gggcctgcgc ggaaatcgcg
    12421 atggcccagc aggcgagcaa aatggccgat gatgtgcgca tcgcaagttt tcagaatgta
    12481 agaagggttg attattatgg atttgggtat cgggggcaat tgggcgctgg tgtgcggcgc
    12541 gagcaagggc ctgggcttcg gctgcgcgca ggcgctggtg cgcgaaggcg tgaacgtggt
    12601 cctggtggcg cgcggcgcgc aggcgctgga ggcggcggtg caacagctga ttgccgatcc
    12661 cgcccggccc gcggataccg cagtgcaaca tgtggcggcc gatatcacca ccgccgaggg
    12721 ccgcgccaag gtgttcgagc atcggcgcga tttcgatatc gtggtcacca acgccggcgg
    12781 gccgccgccc ggcgacttcc gcagctggga ccgcgaagcc tggatcaagg cggtggatgc
    12841 caacatgctg acgccgatcg agttgatcaa ggcgacggtg gacggcatgg cggcgcgggg
    12901 gttcgggcgc atcatcaaca tcacgtcgag cgccgtgaag gcgccgatcg atattctcgg
    12961 cttgtccaac ggcgcgcgca gcggcctgac cggcttcgtg gccggcgtgg cgcgcaccag
    13021 caagctggcc tcggccaacg tcaccatcaa caacctgctg cccggcgcgt tcgacaccga
    13081 ccgcctcaag gccacgatgg ccggcgcgtc cgagaagacc ggcaagccga tcgaggtggt
    13141 gatgcaagcg cggcgcgcga ccattccggc gcggcgcttc ggcacccccg aggagttcgg
    13201 cgcggtttgc gccttcctgt gcagccggca tgccggctac atcacgggcc agaacatcct
    13261 gaccgacggc ggcgcttacc ccggcacata ctgaggcgag gaagcgctcg cctcgaggga
    13321 gcgttttttt tccatgtcct gccgccgcgc agtgcttgcg gtttcgtggc aggatgcgcg
    13381 cctgactttc gcgaggaaac atcatgagca gagcagtaca aatcgcccag cacggcggcc
    13441 cggaacagtt gaagatcgcc gaagtaggcg tgggcgagcc cggtcccggc gaaatccgta
    13501 tccgccaccg cgcggtgggc ctgaacttca tcgacgtgta ccaccgcacg ggcctgtacc
    13561 cgctgaaaat gccggcgacg ctgggcatgg aagcctccgg cgtggtggag gcggtgggcg
    13621 agggcgtgca gcacctcaag gccggcgacc gcgccgccta tgcgagccag cccccgggca
    13681 gctattgcga gctgcgcgtg atgccggcca agtgcgtttg caggctgccc gagccaatag
    13741 acttcgacac ggccgcggcc atgatgctca aggggctcac ggcgcagtac ctgctcaagc
    13801 gcacccagcc gcaaggcgga ctgaagcaag gcgacttcgt gctgttccat gccgcggccg
    13861 gcggtgtcgg cctgatcgcc tgccaatggg cgcgggcgat gggcctgcag ctgatcggca
    13921 cggccggctc ggacgcgaag tgcgcgctgg ccagggagca cggcgcggcg catgccatca
    13981 actattcgac ggaagatttc ctggcccggg tgaaagagat caccggcggc aaaggcgtca
    14041 aggtcgttta cgactcggtg ggcaaagaca cctgggagaa atcgctcgac tgcctgcagc
    14101 cgttcggcct gatggcatgc ttcggcaatg cgtccggacc ggtgccgccc ttcgcgccgg
    14161 gcatcctcgc gcccaagggt tcgctctatg tcacgcgcca gacgctgttc acccacatcg
    14221 cgacccgcga ggccacgcaa gccatggccg acgacctgtt cgaagccgtg accagcggca
    14281 aggtcaagat caggatcgac cagcgctacc cgctggaaca ggtgcagcag gctcaccgcg
    14341 acctggaagc gcgcaagacc acgggttcga ccatcctgac cctgtgagcc ggccc
//
LOCUS       DASWIY010000127         6437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_443272, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000127 DASWIY010000000
VERSION     DASWIY010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443272"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VGA59_04905"
     CDS             <1..449
                     /locus_tag="VGA59_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug ABC transporter ATP-binding protein"
                     /protein_id="HEX9719050.1"
                     /translation="PTAGVDVTLRQEMWQLVRSLRETGVTIILTTHYIDEAEVMADRI
                     GVISKGEIILVEDKAELMRKLGRKQLTLQLQQPMASIPAELSALPLELAGGGSELTYT
                     YDTHSEHSGIAALLEDLDRAGIGFKDLQTRQSSLEDIFVSLVREHP"
     gene            446..1207
                     /locus_tag="VGA59_04910"
     CDS             446..1207
                     /locus_tag="VGA59_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9719051.1"
                     /translation="MNLYAIRAIYRFEMARTWRTLLQSIVSPVVSTSLYFVVFGAAIG
                     SRIPEAGGVSYGAFIVPGLVMLSILTQSVSNASFGIYFPKFTGTIYELLSAPVSTLEI
                     VLSYVGAAATKSVLLGLIILATAALFVPLRIMHPGWMLLFLVLTTLTFSLLGFIIGIW
                     ADGFEKLQFVPTLIITPLAFLGGSFYSIDMLPPLWQKVTLFNPVVYLISGFRWSFYGV
                     ADVGVGISLAMTALFLAICLAIVTWIFRTGYRLKN"
     gene            complement(1681..2982)
                     /locus_tag="VGA59_04915"
     CDS             complement(1681..2982)
                     /locus_tag="VGA59_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9719052.1"
                     /translation="MNRYPGWERRAVSARDVVAHLESRQKVFVHGAAATPTALLDAMV
                     ERTDLEGVELFHLHLSGPCRFANERYRDAFISNPLFTDHGMRRPVEEGRAFFVPVFLS
                     DVPALFATRRIPLDAALVSLSPPDEHGLCTLGTSIDAAKTATEHATIILAEINEQLPR
                     THGATAVPFERVTAFVHTDRPLHEAPAGEIGAVANQIGEHIAKLVEDGSTLQMGIGAI
                     PNAVLSRLGNKHDLGVHTEMFSDLLVPLIEAGVVTNRHKKVHPGRTVTSFVNGTRALF
                     DFVHDNQLVEFHPCDRTNDTALIRKNPRVVAVNAALEIDLTGQVCADSIGHSIYSGIG
                     GQMDFIRGAALSEGGKAIIALPATARSGRISRIVSTLKPGAGVVTTRGHVQYVATEYG
                     IVDLHGLNLKQRAQALISIAHPDFRAELTREVREIRHFDMG"
     gene            3158..3616
                     /locus_tag="VGA59_04920"
     CDS             3158..3616
                     /locus_tag="VGA59_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719053.1"
                     /translation="MHADLGGQGEDRVFFWNVHGAVGLNGENRMEDVLFVSWCIFKMR
                     AWSHVPEATKTILRKAPLSEHCSGREDDPLVVAIKAIQRMNPAMKVDGRVSPVVNATG
                     TYRHQGRNTLFLIFGLNNALKDLHPQQFPRIDLMPEFAWQLKKAATLPFS"
     gene            complement(3641..4981)
                     /locus_tag="VGA59_04925"
     CDS             complement(3641..4981)
                     /locus_tag="VGA59_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9719054.1"
                     /translation="MLHSSTHDTIDAVWRIDSAKIVAGVARMVRDIGVAEELAQDALV
                     AALEHWPLDGVPRNPGAWLMATAKNRALDRLRRDKSLQAKLEQIGHDLEAQEALIVPD
                     FVDALDDARADEIGDDLLRLIFTACHPVLSTEARVALTLKLLGGLTTHEIARAYLVPE
                     PTIAQRIVRAKRAVGEARVPFELPRGNALGERLASVLEVVYLIFNEGYTATAGEDWMR
                     PGLCDEALRLGRMLAELAPRQSEVHGLVALMELQASRAPARVDSTGRPVLLLEQDRAR
                     WDPLLIRRGLAALDRAESLSGGAPGRYALQAAIAACHARAHTAGATDWTRIAAIYDAL
                     AQVAPSPVVELNRAVAQAMAFGPAAGLEIIDKLSGEKSLRNYQWLPSVRGDLLAKLGR
                     NDEARAEFERAASLATNARERDLLLDRARKLEGALAPFSKSLAPSQAAGREPRG"
     gene            complement(4992..5462)
                     /locus_tag="VGA59_04930"
     CDS             complement(4992..5462)
                     /locus_tag="VGA59_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9719055.1"
                     /translation="MINTVSTPSSQAADPREIVSSRLIDAPREKVFKAFSDPTHLARW
                     WGPSGFSNTFEEFDFRPAGIWRVTMHGPDGTDHPNRSVFLDIQEPSRIVFEHESPHVF
                     RMTITLADESGKTRISWRMLFESAAARDQVAPYVVSANEQNFDRLEAELPLVNL"
     gene            complement(5472..5894)
                     /locus_tag="VGA59_04935"
     CDS             complement(5472..5894)
                     /locus_tag="VGA59_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9719056.1"
                     /translation="MRFMIIVKATPDSEAGRFPDDSEKMFAAMADYHEQLAKAGVLLD
                     GSGLQPSSKGWRIRYDGDRRTVVDGPFTESKELIAGYTLIQVRSRAEALEWSRRFPNP
                     VGENKPAEIEVRQLYEMDDFAGLVEPATADRLKEIGIK"
     gene            complement(6013..6366)
                     /locus_tag="VGA59_04940"
     CDS             complement(6013..6366)
                     /locus_tag="VGA59_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9719057.1"
                     /translation="MPYMLLIHEPVGQRQARSRAEGEAVYERMLRFAADLESRGVLRA
                     VESLASQDGAARIKVSNGRAHVLDGPFAEAKEMVGGFFLLDVETRGEAVAIAGECPAA
                     EWATVEVRAVGPCYT"
BASE COUNT         1056 a         2092 c         2124 g         1165 t
ORIGIN      
        1 agcccactgc cggcgtggac gtcacgctgc gccaggagat gtggcagctg gtgcgctcgc
       61 tgcgggaaac cggcgtcacg atcatcctga ccacgcacta catcgacgaa gccgaagtga
      121 tggccgaccg catcggggtg atcagcaagg gcgagatcat cctggtggaa gacaaggccg
      181 agctgatgcg caagctcggc cgaaagcagt tgacgctgca gctgcagcag ccgatggcga
      241 gcattccggc cgagctgtcg gcgcttccac tggagctggc gggcggcggc agcgagttga
      301 cctacaccta cgacacgcac agcgagcatt cggggatcgc ggcgctgctg gaggacctgg
      361 accgggcagg gatcggtttc aaggacctgc agacccggca gagctcgctg gaagacattt
      421 tcgtcagcct ggtgagggag catccatgaa cctttacgcc atccgcgcca tctaccgctt
      481 cgagatggcg cgcacctggc gcacgctgct gcaaagcatc gtctcgccgg tggtgtccac
      541 ttcgctgtat ttcgtggtgt tcggcgcggc gatcggctcg cgcattcccg aggcgggcgg
      601 ggtgagctac ggcgccttca tcgtgcccgg gctggtgatg ctgtcgatcc tgacgcaaag
      661 cgtgtcgaac gcgtcgttcg gaatctattt cccgaagttc acgggcacga tctacgaact
      721 gctgtcggcg ccggtgtcca cactggagat cgtgctgagc tatgtcggcg cggcagccac
      781 caagtcggtc ctcctgggct tgatcattct cgcgaccgcg gccctgttcg tgccgctgcg
      841 catcatgcat ccgggctgga tgcttctgtt cctggtcctc acgaccctga cgttcagcct
      901 gctgggtttc atcatcggca tctgggccga cggtttcgag aagctccagt tcgtgccgac
      961 cttgatcatc acgccgctgg cgttcctggg cggcagcttc tactcgatcg acatgctgcc
     1021 gcccctgtgg cagaaagtca ccttgttcaa ccctgtcgtg tacctgatca gcggctttcg
     1081 ctggagcttc tatggcgtcg ccgacgtggg cgtcggcatc agcctggcga tgacggcgtt
     1141 gttcctggcg atctgccttg ccatcgtgac ctggatcttc aggaccggct accggttgaa
     1201 gaactgatcc ggcgccaccg aggaactgtt ggacccggga tccaggactt cctggattgc
     1261 gggtcaagcc cgcaatgaca atgcctcccg gctgtcatcc cggacttgat ccgggatcca
     1321 tgacttctga attcctggat tggggccggg ccggagcggc catgcctcgg tacgtgcata
     1381 gcgtaggccc agcattgggc ttgctggact ggacagctgc gcgccccggg cgttccgcga
     1441 gcccagcccg caatgacaaa tcaggagagc ttgtcatccc gggcttgacc cgggatccat
     1501 gacttcctgg attgcggggc cgggccggtg cgtgatgcct cggcaccggg ccggagcggc
     1561 catgcctcgg tacgtgcata gcgtaggccc ggcattgggc ttgctggact ggacagctgc
     1621 gcgccccggg cgttccgcga gcccagcccg caatgacaca ccttctcggc attcgtggca
     1681 tcaacccata tcgaagtgcc ggatctccct cacctcgcgc gtgagttccg cgcgaaagtc
     1741 cggatgcgcg atcgagatca gcgcctgggc ccgctgcttc aggttcaacc catgcaggtc
     1801 gacgatgccg tattcggtgg ccacgtattg cacatggccg cgcgtcgtta ccacgccggc
     1861 gcccggcttg agcgtggaca cgatgcggga gatccggccg ctcctggccg tcgcgggcag
     1921 cgcgatgatg gccttgccgc cttcggacag cgccgcgccg cggatgaagt ccatctggcc
     1981 gccgatgccc gagtagatgc tgtgcccgat cgagtcggcg cacacctgcc cggtcaggtc
     2041 gatctccagc gccgcgttga cggcgaccac ccgcgggttc ttgcggatca gcgccgtgtc
     2101 gttggtgcgg tcgcacggat ggaactcgac cagctggttg tcgtggacaa agtcgaacag
     2161 cgcgcgcgtg ccgttgacga aactcgtgac ggtgcgtccc gggtgcacct tcttgtgccg
     2221 gttggtcacc acgccggcct cgatcagcgg aaccagcagg tcggagaaca tttcggtgtg
     2281 cacgcccagg tcgtgcttgt tgcccagccg gctcagcact gcgttgggaa tggccccgat
     2341 gcccatctgc agcgtcgagc cgtcttcgac cagtttcgcg atgtgctcgc cgatctggtt
     2401 cgccacggcc ccgatctcgc cggccggcgc ttcgtgcagc ggccggtcgg tgtggacgaa
     2461 cgccgtcacg cgttcgaacg gaaccgccgt ggcgccgtgc gtgcgcggca gctgctcgtt
     2521 gatctcggcc aggatgatcg tcgcgtgttc ggtggcggtc ttggccgcgt cgatcgacgt
     2581 tcccagggtg cacaggccat gctcgtccgg cggcgacaac gagaccaggg cggcatcgag
     2641 cggaatgcgg cgggtcgcga acagcgccgg gacgtccgag agaaagaccg gcacgaaaaa
     2701 ggcgcggcct tcctcgaccg gcctgcgcat tccatggtcg gtgaagagcg ggttggagat
     2761 gaacgcgtcc cggtacctct cattggcgaa gcggcagggg ccactgaggt gaaggtggaa
     2821 cagctcgacg ccttcgaggt cggtgcgctc caccatggcg tcgagcagcg ccgtgggcgt
     2881 tgcggcggcg ccatggacga acactttctg acggctctcg aggtgcgcca ccacgtcgcg
     2941 cgcactgacc gcgcgccgct cccagccagg atatcgattc attgaattcg tattgttggt
     3001 tgccgattcg agcgcacagg ttagcagttt cgcgcacggc cggtccgggc agccggcgag
     3061 ttcatccaaa gatcggggag aagcgatccg ggaacggctt tcgtaattca acgatgcggt
     3121 ctagaatttt gcgacctgaa cacaacggag ttgcgccatg catgctgatc tcggcggcca
     3181 gggggaggat cgagttttct tctggaatgt ccacggggcg gtcgggctga acggcgagaa
     3241 tcgcatggaa gacgtgctgt tcgtatcgtg gtgtattttc aagatgcgag cgtggagcca
     3301 cgtccccgag gccacaaaga caatcctgcg aaaagcgcct ctcagcgagc attgttcagg
     3361 gcgcgaggac gatccgctgg tcgttgccat caaggccatc caacgcatga acccggccat
     3421 gaaggtggac ggccgagtca gcccggtcgt caacgccacc ggcacctatc ggcaccaggg
     3481 gcgcaacacc cttttcctga ttttcggcct caacaatgcg ctgaaggatc tgcatccgca
     3541 gcagttccca cgcatcgatc tcatgccgga gttcgcgtgg cagctcaaga aggcggcgac
     3601 cttgccgttc tcatgagcac gctgctcctg ccggccgcgt tcagccgcgc ggttccctgc
     3661 cggcggcttg gctcggtgcc agagatttcg aaaacggcgc gagggcgcct tccagcttgc
     3721 gggcgcggtc gagcagcaga tcgcgctcgc gtgcattggt cgccagcgaa gcagcgcgct
     3781 cgaactctgc ccgcgcctcg tcgttgcggc ccagcttggc caggagatcg ccgcgcacgc
     3841 tcggcagcca ttgatagttt cggagcgatt tctcaccgct gagtttgtcg atgatttcca
     3901 gcccggcagc cgggccgaac gccatggctt gtgcgacggc acgattgagt tccaccacgg
     3961 gtgacggcgc cacttgcgcc agtgcgtcgt agatcgccgc gatgcgcgtc caatcggtgg
     4021 cgccggcagt atgggcgcgc gcatgacagg cggcgatggc ggcttgcagc gcatagcggc
     4081 caggcgcgcc gccactcagc gattcggcgc gatccagcgc ggccaggccg cggcggatga
     4141 gcagcgggtc ccagcgcgcc cggtcctgct ccagcagcag gaccgggcgt cccgtcgagt
     4201 cgacacgcgc gggcgcacgc gaggcctgca gttccatcag cgcgaccaat ccgtgcactt
     4261 cggattgccg cggcgccagc tccgccagca tcctgcccag ccgcaatgct tcgtcgcaca
     4321 agccggggcg catccagtcc tcgcccgccg tggcggtgta gccctcgttg aagatcaggt
     4381 acaccacctc gagcacggac gccagccgtt cccccagcgc attgccgcgc ggcaactcga
     4441 acggcacacg ggcttcgccc accgcgcgct tggcacgcac gatgcgttgc gcgatggtgg
     4501 gctcgggcac caggtaggcg cgcgcgatct cgtgagtcgt gaggccaccc agcagcttga
     4561 gcgtcagcgc gacgcgcgct tcggtggaaa gcacgggatg gcacgcggtg aagatcagcc
     4621 gcagcaggtc gtcgccgatc tcgtcggcac gcgcgtcatc cagcgcatcc acgaagtccg
     4681 gcacgatcag cgcctcctgg gcttccaggt catgaccgat ttgctccagc ttggcctgca
     4741 gcgacttgtc ccgccgcagc cggtccagcg cccggttctt tgcggtggcc atcaaccagg
     4801 cgccggggtt tcggggtacg ccgtccagcg gccagtgctc cagcgccgcc accagcgcgt
     4861 cctgcgcgag ctcttcggcc acgccgatgt cgcgcaccat ccgcgcgacg ccggcgacga
     4921 tcttcgcgga gtcgatgcgc cagaccgcgt ctatcgtgtc gtgcgtgctc gaatgaagca
     4981 attcagctct gtcataaatt gaccaaaggg agttcggcct ccagccggtc gaagttctgc
     5041 tcgttcgcgg acaccacgta cggcgcgacc tgatcgcgcg ccgccgccga ttcgaacaac
     5101 atgcgccagc ttatgcgcgt cttgcccgac tcgtcggcca gcgtgatggt catgcgaaat
     5161 acgtgcggag actcgtgctc gaacacgatg cgcgaaggct cctggatgtc caggaagacg
     5221 ctgcggttcg ggtgatcggt gccgtccggc ccgtgcatcg tgacgcgcca gatgccggcg
     5281 gggcgaaagt cgaactcctc gaaggtgttc gagaagccgc tcggccccca ccagcgcgcc
     5341 aggtgggtcg gatcggagaa ggccttgaac accttctcgc gcggcgcatc gatcaggcgg
     5401 ctgctgacga tctcgcgcgg gtcggcggcc tggctggatg gggtcgagac ggtgttgatc
     5461 atggcctggc cttacttgat gccgatctcc ttgaggcggt cggcggtcgc cggctcgacg
     5521 agtccagcga aatcgtccat ctcatagagc tgccgcacct cgatctcggc gggtttgttt
     5581 tcgcccaccg ggttcgggaa gcggcgtgac cactccagcg cctcggcgcg gctgcgcacc
     5641 tggatcaggg tgtagccggc gatgagttcc ttgctctcgg tgaaagggcc gtccaccacg
     5701 gtacgccggt cgccgtcgta acggatgcgc cagcccttgc tgctgggctg caagcctgag
     5761 ccgtcgagca gcacaccggc cttggcgagc tgttcgtggt agtcggccat ggctgcgaac
     5821 attttctcgc tgtcgtccgg gaagcggccg gcctcggagt ccggcgtggc cttgacgatg
     5881 atcatgaatc tcatgggatg ctcctttgct gttgaggtag ccgaagcggc ggaaaatcgc
     5941 gcctctaggc tagggcctgt tcacacgacg aagcaggccg gcgggaatcg acaacgccgg
     6001 ctacggaaac aactacgtat agcagggacc cacggcgcgc acctcgaccg tggcccattc
     6061 ggccgccggg cattcgccgg caatggcgac ggcttcgccg cgcgtttcaa catccagcag
     6121 gaaaaagccg ccgaccattt ccttggcctc ggcgaaaggg ccgtcgagca catgggcccg
     6181 cccattgctg accttgatgc gcgccgcacc gtcctgcgac gcgagcgact cgaccgcgcg
     6241 cagcacgccg cgggactcga ggtcggcggc aaagcgcagc atgcgctcgt acaccgcctc
     6301 gccttcggcc cggctgcggg cttgccgctg gccgaccggc tcgtggatca acaacatgta
     6361 tggcatggga cgggcatgat aagccagacc cgcaagctct tgcaaaagcg cgcgccgagg
     6421 ccatttaccc ttcttcc
//
LOCUS       DASWIY010000128        13379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_810249, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000128 DASWIY010000000
VERSION     DASWIY010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 13379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13379
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810249"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /locus_tag="VGA59_04945"
     CDS             <1..97
                     /locus_tag="VGA59_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719058.1"
                     /translation="VVDLLIGLRVTEEEEREGLDISSHGETAYNR"
     gene            complement(218..907)
                     /locus_tag="VGA59_04950"
     CDS             complement(218..907)
                     /locus_tag="VGA59_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719059.1"
                     /translation="MQEQQQDKSGSAGDWVRDRTVEAARYALLRRLAYAMRHHMVVHL
                     QPIGMITEVMERRLRQPSPDLAQVHDSMSRINGFSRAAVQSCLDVVSWMAPEDGALIA
                     LDAGVAECVGMLRSNFNFRGFTLKDEIGGAAVEVSRAGLRNVLPASMLALTDGAASPA
                     DVVISARLEAGQAVLTIALRPTGGAAGFDGDKPYRRLDWHEVQALAQADGLGLERDGQ
                     QVRVRVPSAAA"
     gene            1017..1871
                     /locus_tag="VGA59_04955"
     CDS             1017..1871
                     /locus_tag="VGA59_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEX9719060.1"
                     /translation="MFQLQSGALVCEIEPRLGGCIAGLWLGGVPVLRSRPAAQLTSAR
                     QAGSYPLVPFSNRIAQASLLWQGTQHPLVRNNGEEPHAIHGVGWQRPWEVLDSDSDFA
                     LLAYEHRPDAAWPFAFDSSQTLRLRGGALELTLSLTNQSRRPAPAGLGWHPYFVKRSR
                     SRISFEATGRWEMGPDKLPTERRPAHGLDAPCGFLDVDHCFDGWTGTVHLRDELLHTR
                     VTSGLSRLVVFTNDTRDFVAIEPVSHVNNAMNLVAGGADPAGLGLAILEPGDSISAQM
                     AIAVERVR"
     gene            2016..2939
                     /locus_tag="VGA59_04960"
     CDS             2016..2939
                     /locus_tag="VGA59_04960"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9719061.1"
                     /translation="MTDCRTAVSQPDELGESPFWHPGERLLYWVDIPARQIRRTDPAV
                     GMIESWEMPSEPGCIAPVRGGGLVLALRDGVYRASSWGGTLTPVARFSHDVATTRFND
                     GKADPVGRFWAGTMYEPRDARKAELYSIDMRGSSGGEPLVQLKAQNAVIANGLAWSPD
                     ARTLYWADTPNHVIHAWDWEAQGNVMSHHRVFQQFPGKPALWQPGQPGYGGRPDGAAV
                     DVEGNYWCAMFEGARLLKFAPAGRLIAEFPLPVRCPTMPCFGDADLKTLYVTSARHNR
                     PAEELQALPQSGCVIAMRVEVPGLPVNFVSD"
     gene            3002..4099
                     /gene="glcE"
                     /locus_tag="VGA59_04965"
     CDS             3002..4099
                     /gene="glcE"
                     /locus_tag="VGA59_04965"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEX9719062.1"
                     /translation="MELILDQLAGRVRAAADNARPLRIRGGGSKDFYGEPPQGELLET
                     APLAGIISYEPTELVVTARAGTPLAALEALLAERGQCLPFEPPHFGGGATVGGMVAAG
                     LSGPARASAGSARDFVLGLTMINGRGEVLTFGGQVMKNVAGYDVSRLVAGSLGTLGLI
                     AEVSLKVLPVAPGEATLKFEMTQAEALRRLNDWGGKPLPLNASCWVDDAGVSTLFLRL
                     RGAVAAVEAACKSLGGERQDNAQVAADWALCRDQRLPWFNERGSRDLWRLSVAQTAPV
                     LELPEPPLIEWHGGQRWVRAEPRDADRLREAARRGGGHATLFAPASDRSAPATGRFAA
                     LAPPLDRIHRELKREFDPAGIFNRGRMYPDF"
     gene            4118..5347
                     /gene="glcF"
                     /locus_tag="VGA59_04970"
     CDS             4118..5347
                     /gene="glcF"
                     /locus_tag="VGA59_04970"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEX9719063.1"
                     /translation="MQTHLSPEFKNTPEGQEAEAILRKCVHCGFCTATCPTYQILGDE
                     LDSPRGRIYLIKQVLEGAAPTRKTQLHLDRCLTCRNCESTCPSGVEYGHLVDIGRKIV
                     EKRVPRDATAQALRWTLKEGLSSPLFGPAMKLGQTVRGLLPRALKDKVPARQDAGRWP
                     TRVHARKVLMLAGCVQPSMMPNINGATARVLDAAGIQTVVAAQAGCCGAVKFHLNDQE
                     GGLAQMRANIDAWWPFVESGKVEAIVMNASGCGVTVREYGHHLQHDPAYADKARRVGE
                     LTRDLSELLPDLVAALRARVRPAPGVLAFHPPCSLQHGQQLRGGVERHLNDLGFTVRV
                     AMNESHLCCGSAGTYSVLQPEMAHQLRDRKLGNLAELQPRAIISANMGCIAHLQSGTA
                     VPVKHWVEVLDAALVPS"
     gene            5390..6094
                     /locus_tag="VGA59_04975"
     CDS             5390..6094
                     /locus_tag="VGA59_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX9719064.1"
                     /translation="MRRADRLFHIIQLIRGRRLTTAAYLAERLEVSERTIYRDVADLQ
                     LQGVPVEGAAGVGYRLGAGFDLPPMMFTHDEAKALVASVRMAQVWLDPALAQGAQDAL
                     GKILSVLPVDARVAAESLPVFAPLVGLPAAAQRTLQTLREAVHERRKVFINYRDLADK
                     PSERTLRPLGCFYWGKVWTLAAWCEQRNDFRSFRVDRVTYVRRLEERFRDEPGRTLAD
                     LARLNEARAKEQGWAS"
     gene            complement(6108..6593)
                     /locus_tag="VGA59_04980"
     CDS             complement(6108..6593)
                     /locus_tag="VGA59_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX9719065.1"
                     /translation="MTSIYDFEARQIDGKKVPLKKYKGKVLLIVNTASACGFTPQFGG
                     LEELHQTYGKKGLVVLGFPCNQFGAQDPGSNEEIAGFCQLNYGVSFPMMAKIDVNGPT
                     ADPLYQWVTAEAPGLMGSKAIKWNFTKFLIGKDGQVIKRYAPADPPATLAKDIEAALA
                     A"
     gene            complement(6598..7515)
                     /locus_tag="VGA59_04985"
     CDS             complement(6598..7515)
                     /locus_tag="VGA59_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9719066.1"
                     /translation="MKRGLLPAGGLLPALALVLNAFVWGVSWWPFRELQSHGLHPLWA
                     TALIYLVSMACLVALKPQAWHGFRGQPLLWLLMVAAGMTNVGFNWAVTIGDVVRVVLL
                     FYLMPAWTVVLAWPMLGEKPTPLALLRLALAMTGVAIVLKTGDSPWPVPESAADWLAL
                     MGGFSFALTNILLRKLHRAAPTARILAMFGGGALLATGAALTGLALGHIAAPPAPQAA
                     WAALGLALSLGFLVSNVALQYGASRMNAHAAALIMLSEVVFASLSSVALGAAELTART
                     LLGGALILAAAAWSAWPPGESVPERVDAQ"
     gene            complement(7569..8852)
                     /gene="lplT"
                     /locus_tag="VGA59_04990"
     CDS             complement(7569..8852)
                     /gene="lplT"
                     /locus_tag="VGA59_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEX9719067.1"
                     /translation="MKRGFYTIMSAQFFSSLADNALFIAAVELLKSSRAPTWQAAALV
                     PMFALFYVVLAPFVGAFADALPKGKVMFLSNAIKVVGCVLMLFGLHPLLAYAVVGLGA
                     AAYSPAKYGILTELLPPSQLVKANGWIEGLTIASIILGILLGGQLVGHAVSTRLLAFD
                     FPLFDTGIDTPPEVAIFAMIFIYILAAWFNTRIPHTGVEMRRMPVNPLELVPDFWICN
                     SRLWRDKLGQISLATTTLIWGVSGNLRYIILAWSAAALSYSTTQASSLSGVVALGTAV
                     GAVVASMRMKLDMATSVMPLGIAMGVLMILMNFIGNVWVAAPFLVLLGAIGGFMVVPM
                     NALLQHRGHNLMGAGRSIAVQNFNEQACILGLGAFYSLSAGLGLSAFAAITAFGLIIT
                     GVMWMIQRWHRNNCVQYREEVEHLLQVARHDNIHG"
     gene            8979..10073
                     /gene="alr"
                     /locus_tag="VGA59_04995"
     CDS             8979..10073
                     /gene="alr"
                     /locus_tag="VGA59_04995"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX9719068.1"
                     /translation="MPRPIQAIIHSDALRHNLGRMRASAPGAKVWGIVKANAYGHGIE
                     RAFEGLRAADGFGLLDLEEAGRVRALGWRGPILLLEGVFEARDLELCSRLNLWHVVHC
                     DEQIDMLAMHKTHQPHRVFLKMNSGMNRLGFTPQRIRAAWTRLDALPQVHEISLMTHF
                     SDADGPKGIARQVRVFNEVTRDLPGERSLSNSAASLRYGADAAVRADWIRPGIAVYGS
                     SPDYPEHDIAHWNLQPTMTLATRIIGVQQLVPGDTVGYGSHFTADAPLRIGVAACGYA
                     DGYPRHCGTGTPVLVEGVRTRIVGRVSMDMITVDLTPVPRAGFGSEVTLWGRASNGAV
                     LAIDEPARAAGTLGYELMCALAPRVPVTVE"
     gene            complement(10133..10240)
                     /locus_tag="VGA59_05000"
     CDS             complement(10133..10240)
                     /locus_tag="VGA59_05000"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9719069.1"
                     /translation="MWKYRVGDWRIICDLQDKRIVVRVLRVGNRREVYR"
     gene            complement(10252..11154)
                     /locus_tag="VGA59_05005"
     CDS             complement(10252..11154)
                     /locus_tag="VGA59_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9719070.1"
                     /translation="MTSSRAISGTAFATLLLIALMMGANHVAARIAFNNGVDVATAVV
                     FRSGVTALVLVVLLRLQGAQVRFTRRHRLMLPAIGLLVGIQSLCLYSSVARLPVALAL
                     LAFNTYPIWTAFWARVIYRQRAERALLIAMPVILLGLALALDVLGAASGLGAKGQWAQ
                     IGAGVAFALAAAGTFGLALVITQHETADVDGRVRTATTMSIAGVVALATVAMQGGFHM
                     PTATPGWWGLAILTFLYGTAFTIMFTVLPRLGVVGNSAIMNVEPVFALVMAWLILGQA
                     IAPVQVGGALIVVGAVMALGMRRH"
     gene            complement(11204..11647)
                     /locus_tag="VGA59_05010"
     CDS             complement(11204..11647)
                     /locus_tag="VGA59_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9719071.1"
                     /translation="MSKPARVLHLAEPPSAYLYRPALVVDCSVLSAALFEEETRDDAL
                     RLLTGKSLHAPSLLDHEIVSVALKKGLMGWPEAAIALALADYAQQDIDLHRTDAQTQY
                     QLGLRYRLSAYDAAYLWLAAELKAPLATFDEKLAVAARTHLASLT"
     gene            complement(11644..11985)
                     /locus_tag="VGA59_05015"
     CDS             complement(11644..11985)
                     /locus_tag="VGA59_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719072.1"
                     /translation="MATLSIKGVPENWAAALRERAALNHRSLQGELMAIIEQAAGEAS
                     SQTRARDSVGIPGQGAAGSSDEAMKTMRSTRAGRKTIEQIAQEHRQRFPEPIRGGPLA
                     VDIIREDRDSR"
     gene            complement(12067..13041)
                     /locus_tag="VGA59_05020"
     CDS             complement(12067..13041)
                     /locus_tag="VGA59_05020"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX9719073.1"
                     /translation="MRVCIYGAGAIGGWLGVKLAQAGCDVSVVARGATLEALQSHGLR
                     LQQGGENLSAAVRASAIPAELGAQDLVVIAVKAPAMGEVAKSIAALLGPQTVVLTAMN
                     GVPWWFFEGFGGRHAGTCLQSIDPGGGIARAVPAKHIIGCVVHASCSLQAPAFVRHHF
                     GNKLIIGEPSGEKTARVRDLAALLERAGFETVLSEQIQKDVWFKLWGNMTMNPVSAIT
                     GATTDRVLDDDLVRGFIQSVMLEAKEIGERIGIPIDQSPEDRHGVTRKLGAFKTSMLQ
                     DVEAGKAVELDALVTVVKELGALTGVETPFTDALLGLARLHARVHGLY"
     gene            complement(13249..>13379)
                     /locus_tag="VGA59_05025"
     CDS             complement(13249..>13379)
                     /locus_tag="VGA59_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-2-oxo-heptane-1,7-dioate aldolase"
                     /protein_id="HEX9719074.1"
                     /translation="TDDALARRYLELGATFVAVGLDNQLLVRATSELAAKFKGGGK"
BASE COUNT         2187 a         4596 c         4505 g         2091 t
ORIGIN      
        1 ggtcgtcgat ctgctgatcg gcctgcgggt gaccgaggaa gaggagcgcg aaggcctgga
       61 catctcgtcc cacggcgaaa ccgcctacaa ccgctagcac gcaagcacgc cgcggctgcg
      121 aggccgcggt cccgattttc aagcaaggcc tccattggat gttcggcccg ccagagctta
      181 ggctccagcg ggccgttttt tttgcgccgc cgcccgctca ggcggcagcc gacggcacgc
      241 gcacgcgcac ctgctggccg tcccgctcca gcccgagacc gtccgcttgc gccagcgcct
      301 gcacctcgtg ccagtccagc cgccggtagg gcttgtcccc gtcgaagccg gcggcgcctc
      361 cggtcgggcg cagcgcgatc gtcagcacgg cctgcccagc ttcaagcctg gccgagatca
      421 ccacgtcggc cggggaagcg gctccgtcgg tcaacgcgag catgctggca ggcaggacat
      481 tgcgcagccc ggcgcgcgaa acctcgaccg cggcgccgcc gatctcgtcc ttcagggtga
      541 agccgcggaa attgaaattg ctgcgcagca tgcccacgca ttcagccaca cccgcatcca
      601 gcgcgatcag ggcgccatcc tcgggcgcca tccaggacac cacgtccagg caggactgca
      661 cggccgcccg cgagaagccg ttgatcctgc tcatgctgtc gtggacctgc gccaggtcgg
      721 gcgagggctg tcgcagccgc cgctccatga cctcggtgat catgccgatg ggctggagat
      781 ggaccaccat gtggtggcgc atcgcgtaag cgaggcgcct gagcaacgca tagcgcgccg
      841 cttccaccgt ccggtccctg acccaatcgc cggccgatcc ggacttgtct tgttgttgtt
      901 cctgcatcgc ggcttttccc cctgtacggt caggcttatg ttaccggttc gcggccgctg
      961 ccgggtcgct tgcggcacga tccgggcaca atcgggccag gaggcaggct gccggcatgt
     1021 tccaacttca gtcaggtgcg ctggtgtgcg agatcgagcc gcggctgggc ggctgcattg
     1081 ccggcctgtg gctcggtggc gtgccggtgc tgcgctccag gccggctgcg cagctgacgt
     1141 cggcacggca ggccggcagt tacccgctgg tgcctttttc caaccgcatc gcccaggcca
     1201 gcctgctatg gcagggaacg caacacccgc tggtgcgcaa caacggcgag gaaccccacg
     1261 ccattcacgg cgttggctgg cagcggccgt gggaggtcct ggacagcgac agcgatttcg
     1321 cgctgctggc ctatgagcac cggcccgacg cggcctggcc gttcgcattc gacagctcgc
     1381 agacgctgcg cctgcgcggt ggcgcgctcg aactgacgct ttcattgacc aaccagtcgc
     1441 ggcggccggc gccggcgggc ctgggctggc acccctattt cgtcaagcgt tcgcgcagcc
     1501 ggatttcctt cgaggccacc ggccgctggg agatgggccc cgacaagctg cccaccgaac
     1561 ggcgccccgc gcacgggctg gacgcgccct gcggctttct ggacgtcgac cattgcttcg
     1621 acggctggac cggcaccgtg cacctgcgcg acgagctgct gcacacgcgc gtgacttcgg
     1681 gcctgagccg tctggtggtg ttcaccaacg acacgcgcga cttcgtggcc atcgagccgg
     1741 tgagccacgt caacaacgcg atgaacctgg ttgccggcgg cgccgatccg gccgggctgg
     1801 gcctggccat cctggagccg ggcgattcga tcagcgccca gatggcgatc gccgtggagc
     1861 gcgtgcgatg aaagcccacc cccgaagcgc cttcggcgcc tccccctcaa gggggcgcca
     1921 tcagcggccc ggcaaggccg gttccgcgat ggcccccgca ctgactttag ggtttcatgc
     1981 gccgcggtcg gcgttcagcg ccatggagaa ctgaagtgac cgactgtcgc accgccgttt
     2041 cccagcccga cgagctcgga gagtcgccgt tctggcatcc cggggagcgg ctgctgtatt
     2101 gggtggacat tccggcccgg cagattcgcc gcaccgatcc cgcggttggc atgatcgaaa
     2161 gctgggaaat gccgtcggag cccggctgca tcgcgcccgt gcgcggcggc ggcctggtgc
     2221 tggcgctgcg cgatggggtc taccgcgcca gcagctgggg cggaaccttg acgccggtgg
     2281 cccgcttcag ccatgacgtg gcgacgaccc ggttcaacga cggcaaggcg gaccccgtgg
     2341 ggcgcttctg ggccggcacc atgtacgagc cccgcgatgc gcgcaaagct gagctgtaca
     2401 gcatcgacat gcgcggctcc agcggcggcg agccactggt ccagctgaag gcccagaacg
     2461 ccgtgattgc caatggcctg gcctggtcgc ccgatgctcg cacgctctac tgggccgata
     2521 cgcccaacca cgtcatccat gcctgggact gggaggcgca gggcaatgtg atgagccacc
     2581 accgggtgtt ccagcagttt cccggcaagc ccgcgctctg gcagccgggc cagcccggct
     2641 acggcggccg tccggacggc gccgcggtgg atgtcgaggg caactactgg tgcgcgatgt
     2701 tcgaaggtgc ccggctgctg aagttcgcgc cagcgggccg gctgatcgcg gaatttccgc
     2761 tgccggtgcg ctgcccgacc atgccctgct tcggcgatgc cgacctcaag accttgtacg
     2821 tcaccagcgc acgccacaac cggccggccg aggaactcca ggccctgccg caatcgggct
     2881 gcgtgatcgc gatgcgggtc gaggtgccgg ggctgcccgt caatttcgtc agcgactgat
     2941 ctcaaaaaga tcaatcgccc cgcgctccgg cacgggcctg cccgcgcgta ccatggacgg
     3001 catggaactc atcctcgacc agctggccgg gcgcgttcgc gccgccgccg acaacgcaag
     3061 gccgcttcgc attcgcggcg gcggcagcaa ggatttctac ggcgagccac cgcagggcga
     3121 gctgctggaa accgcgcccc tggccggcat catcagctat gagccgaccg aactcgtggt
     3181 cacggcacgc gcgggcacac ccttggccgc gctggaggcc ctgctggccg agcgtggcca
     3241 atgcctgccg ttcgagccgc cccacttcgg cggcggcgcc acggtgggcg gcatggtggc
     3301 ggcgggcctg agcggcccgg cccgcgcgag cgccggctcg gcgcgcgact ttgtgctggg
     3361 gctgaccatg atcaacggac gcggcgaagt gctgacgttc ggcggccagg tgatgaagaa
     3421 cgtggccggc tacgatgtct cgcggctggt ggcgggctcg ctgggcaccc tgggcctgat
     3481 cgccgaagtc agcctgaagg tgctgccggt ggcgccgggc gaggccacgc tgaagttcga
     3541 gatgacccag gccgaagccc tgcgccgcct gaacgactgg ggcggcaagc cgctgccgtt
     3601 gaacgccagc tgctgggtcg acgacgcggg cgtgtccact ctgttcctgc gcctgcgcgg
     3661 cgccgtggcg gcggtggagg cggcgtgcaa gagcctgggg ggcgaacgcc aggacaacgc
     3721 ccaggtggcg gcggactggg cgctgtgccg cgaccaacgc ctgccctggt tcaacgaacg
     3781 cggcagccgc gacctgtggc gcctctcggt cgcgcaaacc gcgccggtgc tcgagctgcc
     3841 ggagccgccg ctgatcgagt ggcatggcgg ccagcgctgg gtacgcgcgg agccgcgcga
     3901 cgccgaccgg ctgcgcgagg cggccaggcg cggcggcggc cacgccacgc tgtttgctcc
     3961 ggctagcgac cggtccgcgc ccgccaccgg ccgcttcgcc gcgctggcgc cgccgctgga
     4021 ccggatccac cgcgagctca agcgcgagtt cgacccggcc ggtatcttca accggggccg
     4081 tatgtacccc gatttctgac ggcagcgagc cggcaccatg cagacccatc tgtcccctga
     4141 attcaagaac acgcccgaag ggcaggaggc cgaggccatc ctgcgcaagt gcgtgcattg
     4201 cggtttttgt accgccacct gccctacata ccagatcctg ggcgacgaac tcgacagccc
     4261 gcgcgggcgc atctacctga tcaagcaggt gctggaaggc gccgcgccga cccgcaagac
     4321 ccagttgcac ctggaccgct gcctgacctg ccgcaactgc gagagcacct gcccgagcgg
     4381 tgtcgaatac gggcacctgg tcgacatcgg ccgcaagatc gtggaaaagc gggtgccgcg
     4441 cgacgccacc gcccaggcgc tgcgctggac gctgaaggaa gggctgtcgt cgccgctgtt
     4501 cggccccgcg atgaagctgg gccagaccgt gcgcggattg ctgccgcgcg cgctgaagga
     4561 caaggtgccg gcccggcagg atgcgggccg ctggcccacg cgcgtgcatg cgcgcaaggt
     4621 gctgatgctg gccggctgcg tgcagccttc gatgatgccg aacatcaacg gtgcgaccgc
     4681 gcgcgtgctc gacgccgcgg gcatccagac cgtggtggcc gcgcaggccg gctgctgcgg
     4741 cgccgtgaag ttccacctca acgaccagga gggcggcctg gcgcagatgc gcgccaacat
     4801 cgacgcctgg tggccgttcg tggaaagcgg caaggtcgag gccatcgtca tgaatgcatc
     4861 cggctgcggc gtgaccgtcc gggaatacgg acatcacctg caacacgacc cggcctatgc
     4921 cgacaaggca cggcgcgtgg gcgagctcac acgcgacctg agcgagctcc tgcccgatct
     4981 ggtcgcggcg ctgcgcgcca gggtgcggcc cgcgccgggc gtgctggcgt tccatccgcc
     5041 gtgcagcctg cagcacggcc agcagttgcg cggcggcgtc gagcggcacc tgaacgactt
     5101 gggcttcacc gtgcgcgtgg ccatgaacga atcgcacctg tgctgcggct cggccggcac
     5161 ctattcggtg ctgcagccgg agatggcaca tcagttgcgc gaccgcaagc tcggcaatct
     5221 ggccgagttg caaccgcggg ccatcatctc ggccaacatg ggttgcatcg cgcatctgca
     5281 gagcggcacg gctgtgccgg tgaagcattg ggtcgaggtg ctcgacgccg cgctggtccc
     5341 ctcctgacag cgcttgtcag gagatggcag gcacagtaac gttcctgcca tgcgccgcgc
     5401 cgaccgcctg ttccacatca tccagctgat tcgcggccgg cgcctgacca cggccgccta
     5461 ccttgccgag cggctggagg tgtcggagcg caccatctat cgcgatgtcg cggatttgca
     5521 gttgcagggt gtgccggtcg agggcgccgc cggcgtgggc taccgcctcg gcgccgggtt
     5581 cgacctgccg cccatgatgt tcacccacga cgaggccaag gccctggtcg cctcggtgcg
     5641 catggcccag gtctggctcg acccggcgct ggcgcagggc gcgcaggatg cactcggcaa
     5701 gatcctgtcg gtcctgcccg tcgatgcgcg ggttgccgcc gagtcgttgc ccgtcttcgc
     5761 gccgctggtg ggcctgccgg cggctgcgca acgcaccttg cagacactgc gcgaggctgt
     5821 gcatgagcga cgcaaggtct tcatcaacta ccgcgacctc gcggacaagc cgagcgagcg
     5881 caccttgcgg ccgctgggct gcttttactg gggcaaggtc tggacgctgg cagcctggtg
     5941 cgagcagcgc aacgacttcc gcagcttccg cgtggaccgg gtgacttatg tgagacggct
     6001 ggaagagcgc ttccgcgacg agcccggccg caccctggcc gacctggcgc ggcttaacga
     6061 ggcgagggcg aaggagcagg gctgggcgag ctgacgccgc gcgtccttta ggcggccagc
     6121 gccgcttcga tgtctttcgc gagggtcgcg ggcggatcgg ccggcgcata acgcttgatc
     6181 acctggccgt ccttgccgat gaggaacttg gtgaagttcc atttgatggc cttgctgccc
     6241 atgagcccgg gcgcctcggc ggtgacccat tggtacagcg gatcggccgt cgggccgttc
     6301 acgtcgatct tggccatcat cggaaagctc acgccgtagt tgagctggca gaagccggcg
     6361 atttcttcat tgctgcctgg atcctgcgca ccgaactggt tgcagggaaa gcccagcacc
     6421 acgagcccct tcttgccata ggtctggtgc aattcctcca ggccgccgaa ctgcggcgtg
     6481 aagccgcagg cactggcggt gttcacgatc agcagcacct tgcccttgta cttcttcaag
     6541 ggcaccttct tgccgtcgat ctgccgcgct tcgaaatcgt agatgcttgt catcgcgtta
     6601 ttgtgcgtca acgcgctccg gcacggattc gcccggcggc caggccgacc aggccgccgc
     6661 agccaggatg agcgcgccgc cgagcaaggt gcgggcggtc agttccgccg cgcccagggc
     6721 taccgaggac aggctggcga acaccacctc cgacagcatg atcagcgcgg ccgcatgggc
     6781 gttcatgcgc gacgcgccgt attgcagcgc cacgttgctg accaggaaac ccaggctcag
     6841 ggccaggccc aacgcggccc aggccgcctg cggcgccggc ggcgcggcga tatgtcccag
     6901 cgccaggccg gtcagtgccg cgcccgttgc cagcagggcg ccgccgccga acatcgccag
     6961 gatgcgcgcg gtgggcgccg cccgatggag tttgcgcagc aggatgtttg tcagcgcgaa
     7021 gctgaagccg cccatcagcg ccagccagtc cgcggcactt tcgggcaccg gccagggcga
     7081 gtcgccggtc ttgagcacga tcgccacgcc ggtcatcgcc agcgcgagcc gcagcagcgc
     7141 gagcggcgtg ggtttctcgc ccagcatggg ccaggccagc accaccgtcc aggccggcat
     7201 caggtagaac agcaacacca cccgcaccac gtcgccgatc gtgaccgccc agttgaagcc
     7261 cacattggtc atgcccgcgg cgaccatcag gagccacagc agcggctggc cgcgaaagcc
     7321 gtgccaggct tgcggcttta gcgcgaccag gcaggccatg gacaccagat agatcagcgc
     7381 ggtggcccac aagggatgca ggccgtggct ctggagctcg cgaaaaggcc accaggagac
     7441 gccccagacg aaggcgttga gcaccagggc cagtgccggc agcagcccgc cggccggcaa
     7501 caatccgcgc ttcatgctaa tgccggaatg agccgcgccg cgctcgaatc gacctggtca
     7561 gagccttcct agccatggat gttgtcgtgc cgtgcaactt gcagcaggtg ctcgacttcc
     7621 tctcggtact ggacgcagtt gttgcggtgc cagcgctgga tcatccacat cacgccggtg
     7681 atgatgaggc caaaggcggt gatcgcggcg aacgccgaca gtcccaggcc cgccgacagg
     7741 ctgtagaacg ctccgaggcc gaggatgcag gcctgctcgt tgaaattctg caccgcgatc
     7801 gaacggcccg cgcccatcag gttgtgtccg cggtgctgca gcagggcgtt catcggcacc
     7861 accatgaacc cgccgatggc gcccagcagg accaggaagg gcgcggcgac ccagacgttg
     7921 ccgatgaagt tcatcaggat catcagcacc cccatcgcga ttcccagggg catcacgctg
     7981 gtggccatgt ccagcttcat gcgcatggag gcgaccacag cacccacggc ggtgcccagg
     8041 gcgacgacgc ccgacagcga cgatgcttgc gtcgtgctgt agctcagcgc cgcggcgctc
     8101 caggccagga tgatgtagcg caggttgccg ctcacgcccc agatcagcgt ggtggtggcc
     8161 agcgagatct ggcccagctt gtcacgccac aggcgcgagt tgcagatcca gaaatcgggc
     8221 accagttcca gcggattcac gggcattcgc cgcatctcca cgccggtgtg cggaatgcgg
     8281 gtgttgaacc aggcggccag tatgtagatg aagatcatcg cgaaaatggc gacctcgggc
     8341 ggcgtgtcga tgccggtgtc gaacagcgga aagtcgaagg cgagcaaccg ggtcgacacc
     8401 gcatggccga ccagttggcc gcccagcagg atgcccagga tgatggaggc gatcgtcagc
     8461 ccttcgatcc agccgttggc cttgaccagc tgcgaaggcg gcagcagctc ggtaaggatg
     8521 ccgtacttgg cgggcgagta cgccgccgcg cccagaccca ccacggcata ggccagcagc
     8581 gggtgcaggc cgaacagcat cagcacgcag cccaccacct tgatcgcgtt gctcaagaac
     8641 atgaccttgc ccttgggcag ggcatcggcg aaggcgccga cgaaaggcgc gagcaccaca
     8701 tagaacagcg cgaacatcgg caccagggcc gcggcctgcc aggtcggcgc cctggaggat
     8761 ttcagcagtt ccaccgccgc aatgaataaa gcgttgtcgg ccagcgacga gaaaaactgg
     8821 gccgacatga tggtgtagaa accgcgcttc attggctggg tcgtggcgca tcagccgggc
     8881 tgattggcgc tggtttttgt gctgtctcca agtgacaaag ggttatatca cggtgggtaa
     8941 tgacaaaatc ccccttgacg ccgaccgacg aaccgaacat gccccgaccc atccaggcca
     9001 tcatccattc cgacgcgttg cgccacaatc tggggcgcat gcgcgccagc gcacccggcg
     9061 cgaaagtctg gggcatcgtc aaagccaatg cctatggtca tggcatcgag cgcgcgttcg
     9121 agggcttgcg cgccgcggac gggttcggcc tgctggatct cgaggaggcc gggcgggtgc
     9181 gcgcactggg atggcgcggg cccatcctct tgctggaagg cgtgttcgag gcgcgcgacc
     9241 tggagctgtg ttcgcgcctg aacctgtggc acgtggtgca ctgtgacgag cagatcgaca
     9301 tgctggcgat gcacaagaca caccagccgc accgcgtgtt cctcaagatg aactccggaa
     9361 tgaaccgcct gggctttacg ccgcagcgca tccgcgcggc atggacacgg ctggatgcct
     9421 tgccgcaggt gcacgagatt tcgctgatga cgcatttcag cgacgccgac gggcccaagg
     9481 gcatcgcgcg gcaggtccgc gtgttcaacg aagtcacgcg cgacctgccg ggagagcgca
     9541 gcctgtccaa cagcgccgcg agcctgcgct acggcgccga cgcggcggtg cgcgccgact
     9601 ggatccggcc cggcatcgcc gtgtacggca gctcgcccga ctaccccgag catgacatcg
     9661 cccactggaa cctgcagccc acgatgacgc tggccacgcg catcatcggc gtgcagcagc
     9721 tcgtgcccgg cgatacggtg ggctacggct cccatttcac ggccgacgcg ccgcttcgca
     9781 tcggggtcgc cgcctgtggt tatgccgacg gctacccgcg ccactgcggc accggcacgc
     9841 cggtgctggt cgagggcgtg cgcacccgca tcgtgggccg cgtcagcatg gacatgatca
     9901 cggtggacct gacgccggtg ccgcgagccg gcttcggcag cgaggtcacg ttgtggggcc
     9961 gcgcgtccaa cggcgccgtg ctggctatcg acgagccggc ccgggccgcc ggcacgctgg
    10021 gctatgagct gatgtgcgcg ctggcgccgc gggtgcctgt gacggttgaa tgaactccgg
    10081 caaatgaagg gcggccgcag acatccacag acacccgcgg tcggcacggg ttctaccgat
    10141 aaacctcgcg ccgattgccc acccggagca cgcgcacgac gatccgcttg tcctgcaagt
    10201 cgcagatgat gcgccagtcg ccgacgcgat acttccacat cgcgaaccag cctagtgcct
    10261 gcgcattccc agcgccatca ccgctcccac cacgatcagc gcgccgccca cctggaccgg
    10321 cgcgatggcc tggcccagga tcagccaggc cattaccagc gcgaacaccg gttccacgtt
    10381 catgatcgcc gagttgccga ccacacccag cctgggcagc acggtgaaca tgatcgtgaa
    10441 agccgtgccg taaaggaaag tgaggatcgc cagcccccac cagcccggcg tcgcggtggg
    10501 catgtgaaag ccgccctgca tcgccacggt cgcgagtgcc accacgccgg caatgctcat
    10561 ggtcgtggcc gtgcgcacgc ggccgtcgac atcggcggtt tcgtgctggg tgatgaccag
    10621 ggccaggccg aaggtgccgg ccgcggccag cgcaaacgcg acgccggcgc cgatttgcgc
    10681 ccattgcccc ttggctccca gcccggaagc ggcgcccagc acgtcgagcg ccagcgccag
    10741 ccccagcagg atcaccggca tggcaatcag cagggcgcgc tcggcgcgct gccggtagat
    10801 cacccgcgcc cagaacgcgg tccagatcgg gtatgtgttg aaggccagca gggccagggc
    10861 cacgggcaat cgcgccaccg atgaatacag gcacaggctc tggatgccga ccagcagccc
    10921 gatcgccggg agcatgagcc ggtgccggcg ggtaaagcgc acttgcgcgc cctgcaggcg
    10981 cagcagcacc accagcacca gcgccgtcac gccgctgcga aacaccacgg ccgtggccac
    11041 atcgacgccg ttgttgaagg cgatgcgcgc cgcaacgtgg ttggcaccca tcatcagcgc
    11101 gatcagcagc agcgtggcga atgcggtgcc gctgatggcg cgcgacgagg tcattgctgc
    11161 ggcgtcccga tgggaatcgc gttgctcatg gcgcccagtt cgttcaggtc aggctcgcga
    11221 ggtgtgtgcg cgccgcgacg gcaagctttt cgtcgaaggt ggccagcggc gctttcagtt
    11281 cggcggcgag ccacaggtag gcggcgtcat aagcggacag ccggtagcgc aggccgagct
    11341 ggtattgggt ttgcgcgtcc gtgcggtgca ggtcgatgtc ctgctgcgcg tagtcggcca
    11401 acgccagcgc gatggccgct tcgggccagc ccatgagtcc cttcttcaac gcaacgctca
    11461 cgatctcatg gtcaagcaag gacggagcat gcaagctctt gcccgtgagc agccgcagcg
    11521 catcgtcgcg tgtctcttct tcgaacaggg cggcgctcaa gacgctgcaa tcgaccacca
    11581 acgcagggcg gtagaggtaa gcggaaggcg gctcggcaag gtgcagcacc cgtgcgggct
    11641 tgctcatcgc gagtcccggt cctcgcgaat gatgtccacg gccaatggcc cgccgcgaat
    11701 gggctcggga aagcgctgcc ggtgctcctg ggcgatctgc tctatggtct tgcggccggc
    11761 tcgtgtgctg cgcatggttt tcatggcttc gtccgagctc cccgccgcgc cttggcccgg
    11821 tatcccgacc gaatcccggg ctcttgtttg ggaggacgcc tcgcctgcgg cctgctcgat
    11881 gatggccatc agctcgccct gcagcgagcg gtgattgagc gcggcacgct cgcgcagcgc
    11941 cgcggcccaa ttctcgggaa cgccctttat cgacaaggtt gccatttcga ctccagaatg
    12001 gaactggttc caatttggag ccattatggc atcacactaa cgttcctgca atggcgcttt
    12061 cctgggtcaa tacaggccgt gcacccgggc gtgcagacgg gccaggccga gcaacgcatc
    12121 agtgaacggc gtctcgacgc cggtgagcgc gcccaactcc ttgaccacgg tcaccagcgc
    12181 atcgagctcc accgccttgc ccgcctccac gtcctgcagc atcgaggtct tgaaggcgcc
    12241 cagcttgcgg gtcaccccat gccgatcctc cgggctctgg tcgatcggga tgccgatgcg
    12301 ctcgccgatt tccttggctt cgagcatcac gctctgaatg aagccgcgca ccaggtcgtc
    12361 gtcgagcacg cggtcggtgg tggcgcccgt gatggcgctc accgggttca tggtcatgtt
    12421 gccccagagc ttgaaccaga catccttctg gatctgctcg gacagcacgg tctcgaagcc
    12481 ggccctctcc agcagggccg ccagatcgcg cacgcgcgcc gttttctcgc cggagggttc
    12541 gccgatgatc agcttgttgc cgaaatgatg ccgcacgaaa gccggggcct gcagcgagca
    12601 gctcgcatgc accacgcagc cgatgatgtg cttcgcggga acggcccgcg cgatgccgcc
    12661 gcccgggtcg atcgattgga ggcaggttcc ggcatgccgt ccgccgaagc cttcgaagaa
    12721 ccaccaaggc accccgttca tggccgtgag caccaccgtt tgcggaccca gcaacgcagc
    12781 gatcgatttc gccacctcgc ccatggccgg cgctttcacg gcgatgacga ccaggtcctg
    12841 cgccccgagc tcggcgggga ttgcgctggc tcgcaccgcc gccgacaggt tttcgccgcc
    12901 ctgctgcaaa cgcaggccgt gcgattgcaa agcttcaagc gtggcgccgc gggcgacgac
    12961 gctgacgtcg catcccgcct gtgccagttt cacgcccagc cagccgccga tcgcgccggc
    13021 gccgtagatg cagactctca tggttatgtc atcctcatcc ggtttcgtca tcccggactc
    13081 gatccgggat ccatgcctcg cctgaagcag cgctccgccg tggattgcgg ggccgggccg
    13141 gagcgcgatg cctcggtacg tgcatagcgt aggcccggca ttgggctcgc cgcactggat
    13201 agctgcgcgc cccgggcgtt ccgcgagacc agcccgcaat gacaatcgtc attttccgcc
    13261 gcccttgaat ttcgccgcga gctcgctggt cgcgcgcacc agcagctggt tgtccaggcc
    13321 gacggccacg aaagtggcgc ccagttccag gtatcggcgc gccagggcat cgtccgtgg
//
LOCUS       DASWIY010000129         7605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2304150, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000129 DASWIY010000000
VERSION     DASWIY010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304150"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VGA59_05030"
     CDS             <1..196
                     /locus_tag="VGA59_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9719075.1"
                     /translation="GIATRQMLPLFAIVAPAMLIPSLLGARLYLGIGETGFRKVVLTL
                     LTASGVAMLVSALPVLARRW"
     gene            complement(298..1239)
                     /locus_tag="VGA59_05035"
     CDS             complement(298..1239)
                     /locus_tag="VGA59_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9719076.1"
                     /translation="MSRQAEQELAALRRALEETREQLRASRALAAERQGVLENERAAR
                     VLAERVNSLKDEFLAIVSHELRSPLSAIAGWVHILQSGASPEDFENGLEVIQQSVGVQ
                     TKLIEDLLDISRIASGRLRLVVQPAEPRSFIDAAVEAIRPAADAKGIRVRKVLDLSAG
                     PVAGDPGRLQQVMVNLLTNAVKFTPEQGHVEVALQRRGEQAEITVADTGIGIPAAFLP
                     HVFDRFCQADDSIHSRYGGLGLGLAIVKHLVELHGGNVSAESPGEGRGATFVLRLPIS
                     ARRGEAVAGRPITESAPQAAPAGAANALPAETAHSGA"
     gene            1369..1755
                     /locus_tag="VGA59_05040"
     CDS             1369..1755
                     /locus_tag="VGA59_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719077.1"
                     /translation="MTLRAFVVEDNVAIRNSLVEALSELAGIDTAGVAGSEKAAIAWL
                     SDAANEWDLAIIDLILEPGGSGFGVLNACRNRKPEQKVVVLTGAAIPDVRGKCEALGS
                     DCVFDKSIETDALMDYCLAMARTAQA"
     gene            1995..2441
                     /locus_tag="VGA59_05045"
     CDS             1995..2441
                     /locus_tag="VGA59_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719078.1"
                     /translation="MKHAAILVVDDNPDHLELTVMALAECCDAGDIATASDGVEALDY
                     LFGRGAHASRDTAQQPRLVLLDMKLVRLHGLEVLKAVRQDPRTATIPVVMHSSSTEKS
                     DIAACYAHGANSYVRKDTDYDELRRKMHQLYEFWLRVNERCRPTGT"
     gene            complement(2445..3110)
                     /locus_tag="VGA59_05050"
     CDS             complement(2445..3110)
                     /locus_tag="VGA59_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX9719079.1"
                     /translation="MGLMTTALKIDFVSDVSCPWCAIGLASLERALERLHGEVRADLH
                     FQPFELNPAMPPGGQDITEHLTQKYRSTPQQQAQARDAIRERGAAVGFDFRKEGRGRI
                     YNTFDAHRLLHWAALAAPGRQHALKKALLQAYFTHGLSPADRDVLVQAAGRAGLDPAG
                     ARDVLESGRYAAEVRERQRFYLDRGIHAVPAVIVNDRHLVQGGQPPEVFEQALRRIAA
                     GLA"
     gene            complement(3242..3427)
                     /locus_tag="VGA59_05055"
     CDS             complement(3242..3427)
                     /locus_tag="VGA59_05055"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEX9719080.1"
                     /translation="MDSNTSTPPAQRIDLTREMDVAYWCRVLNVSMAQLREAVHHAGH
                     QVHEVQKYLLRKVPSGH"
     gene            complement(3453..4013)
                     /locus_tag="VGA59_05060"
     CDS             complement(3453..4013)
                     /locus_tag="VGA59_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719081.1"
                     /translation="MPAGPAAAGTQGGANAITLAKYPFAPITMHDRNDHPPHDAQSHA
                     PVPQAGAAPGEAHAQAPRACRILVVDDNKDAAETLAELLRMLGNEVAVALNGSSAVER
                     VAQFGPDVVLLDIGLPDINGYEVARRVRRLEGIGQPRLIALTGWGQQQDKQVAAQAGF
                     DEHWTKPVDVERLQELSRNSARRAIL"
     gene            complement(4018..4395)
                     /locus_tag="VGA59_05065"
     CDS             complement(4018..4395)
                     /locus_tag="VGA59_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719082.1"
                     /translation="MSYPAKTLFAFGCYLLVLGMLLIVFPNIVLQLFGVAPTVEVWIR
                     VTGMLVLILGAYDILASLSELHQFIRWSVPMRASVIFFFAVFVLLGYAPPVLLLFGLI
                     DLACAAWTWVALRRARGAPTLAV"
     gene            complement(4414..5511)
                     /gene="aroC"
                     /locus_tag="VGA59_05070"
     CDS             complement(4414..5511)
                     /gene="aroC"
                     /locus_tag="VGA59_05070"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX9719083.1"
                     /translation="MSGNTFGTLFAVTNFGESHGPAIGCVIDGCPPGMALGEADIQPD
                     LDRRRPGTSRHVTQRQEADRVEILSGVHEGKTTGTPIGLLIRNTDQRSKDYSEIAQTF
                     RPGHADYTYLQKYGLRDPRGGGRASARLTAPMVAAGAVAKKWLREKYGTVFRACMQQM
                     GDIAIAFESWDHVPNNPFFAPVADVAQLESYMDSLRKAGDSCGARIRVTASNVPVGLG
                     EPLFDKLDADIAWAMMGINAVKGVEIGAGFASVTQRGTTHGDSPTPQGFRTNNAGGVL
                     GGISTGQDLEVSIAIKPTSSILSPRETINLKGEATQVVTKGRHDPCVGIRAAPIAEAM
                     LALVVMEHALRQRAQNGDVVSPVAAIPASLP"
     gene            complement(5613..6077)
                     /locus_tag="VGA59_05075"
     CDS             complement(5613..6077)
                     /locus_tag="VGA59_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9719084.1"
                     /translation="MKVSDILRVKGGTLYTVTPDEPLAQAMKLMADKDIGSVVVMERG
                     ELVGMLTFREVILCIVRNGGLVGDSVVRSAMEDHPLTCAPDTELDEVRRLMLESHARY
                     MPVMERRVLMGVISFYDVARAVVDSQNFENRMLKAYITDSRGVEEQSPGPQL"
     gene            6175..7470
                     /locus_tag="VGA59_05080"
     CDS             6175..7470
                     /locus_tag="VGA59_05080"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEX9719085.1"
                     /translation="MPGYSDPGFDTLALHAGAAPDPATGARAVPIHLTTSFVFDSSDH
                     AAALFNLEQSGHVYSRISNPTNAVLEQRVAALEGGVGAISTASGQAALHLSIATIMGA
                     GSHIVASTALYGGSQNLLHYTLRRFGIATTFVKPGDIDGWRAAIRPDTKLLFGETVGN
                     PGLEVLDIPRVAAIAHEAAVPLLVDSTLTSPYLMRPLALGADLVYHSATKFLSGHGTV
                     IGGVVVDGGSFDWERSGKFPELTAPYDGFHDMVFSEESTVGAFMLRARREGLRDFGAC
                     MSPHTAWLILQGIETLPLRMERHMHNTRKVVEFLAAHPFVAHVGHPMLESHPSHALAR
                     ELLPKGAGSVFSFDMKGNREQGKKFIEALKLFSHLANVGDCRSLVIHPASTTHFRMSD
                     EALAAGGITQGTIRLSIGLEDPDDLIDDLKRALKAAEKA"
BASE COUNT         1223 a         2564 c         2547 g         1271 t
ORIGIN      
        1 cggcatcgcc actcgccaga tgctgccgct gttcgccatc gtcgcgccgg ccatgctcat
       61 cccttccctg ctcggcgccc ggctgtacct gggcatcggc gaaaccggtt ttcgcaaggt
      121 cgtcctgact ttgctcaccg cctccggcgt ggccatgctg gtctcggcgc tgccggtgct
      181 tgcccggcgc tggtagcgcg cgccgttcgc cgctgctctt gagaaaccgg cgggcagcgg
      241 ctaatccaag ccgaggcgta tcgaagtgcg ggtggggtag tgtgctctcg ggcaccccta
      301 ggcgcccgag tgcgcggttt cggccggcag ggcgttggcg gcacccgccg gtgcggcttg
      361 cggcgcggac tcggtgatgg gccgacccgc aacggcttcg ccgcgcctgg ccgaaatcgg
      421 cagccgcagc acaaacgtgg cgccgcggcc ctcgccgggg ctctcggcgc tgacgttgcc
      481 gccgtgcagt tccaccaggt gcttgacgat ggcaagcccc aggccgaggc caccatagcg
      541 gctgtgaatg gagtcgtcgg cctggcagaa gcggtcgaac acgtgaggca ggaaagccgc
      601 cgggataccg atgccggtgt ccgccacggt gatttccgcc tgttcgccgc gccgctgcaa
      661 ggccacctcg acatggccct gctccggcgt gaacttcacc gcgttggtca gcaggttcac
      721 catcacctgc tgcagccggc cggggtcgcc cgccaccggg cccgccgaga gatcgagtac
      781 cttgcgcacg cggatgcctt tggcgtcggc ggcgggccgg atggcttcca cggccgcgtc
      841 gatgaacgag cgtggctccg ccggttgcac cacgaggcgc agccgcccgg acgcgatacg
      901 gctgatgtcc agcaagtcct cgatcagctt ggtttgcacc cccacactct gctggatcac
      961 ttccaggccg ttctcgaaat cttccgggct cgcccccgac tggagaatat ggacccagcc
     1021 cgcgatcgcg ctcagcggac tgcgcagctc gtgcgaaacg atcgcgagaa attcgtcctt
     1081 gagcgagttc acgcgctctg ccaggacccg ggccgcccgc tcgttttcca ggaccccctg
     1141 gcgctccgcg gccaaagcgc gcgaagccct caattgctcg cgcgtttctt ccagggcccg
     1201 tcgcagcgcc gccagttcct gctctgcttg ccggctcata caagcacccc acgccgcctc
     1261 gggtagcgtg caaaatgact tgaatctgcg ccccgcatgc ctcgcggcgg tgcgtggagc
     1321 aggtggtcat tggctgccgc cgcggcggca gcagcaagga gcccacaggt gacactcaga
     1381 gcattcgttg ttgaagacaa cgtggcgatc cgcaacagcc tggtcgaggc gctgtccgaa
     1441 ctggccggca tcgataccgc cggcgtggcc ggcagcgaaa aagcggcaat cgcgtggctc
     1501 tcggacgcag ccaacgaatg ggatctcgcc atcatcgacc tgatactgga gcctggaggc
     1561 agcggctttg gcgtgctcaa cgcctgccgc aaccgcaagc cggagcagaa ggtggtggtg
     1621 ctgacaggcg cggccattcc cgacgtgcgc ggcaaatgcg aagctctggg cagcgattgt
     1681 gtgtttgaca aatccattga gaccgatgca ctgatggact actgcctggc gatggcccgg
     1741 actgcccagg cctgaggccc cggggggcct atgcgcatca ttgtgtcggc gcagggcctt
     1801 cgcttctgta ggacgcgacg ccgtcgctgc gctgcgataa agggggcggc cggaccggcc
     1861 gatcgcgccc gcttgggccg ctcaggcgat gtgcgaaaat acctgccgag ccgggcgcat
     1921 gcgaccggct gccgccttca gggagagtcc ggccaaacgc tggaagaaaa acgcgctttc
     1981 gtttgggctc gacgatgaaa cacgccgcga tcctcgtggt ggacgacaat ccggatcacc
     2041 ttgaactgac cgtcatggcg ctcgccgagt gctgtgatgc cggggacatc gccacggcga
     2101 gcgacggggt ggaagcgctg gactacctgt tcggccgcgg cgcgcacgcc tctcgcgaca
     2161 cggctcagca gccgcggctg gtgctcctgg acatgaagct ggtccgcctg cacgggctgg
     2221 aggtgctcaa ggccgtgcgg caggatcccc gcacggccac gatccccgtg gtcatgcact
     2281 cgtcctcgac cgaaaagagc gacatcgccg cctgctacgc gcacggcgcc aacagctacg
     2341 tgcgcaagga caccgattac gacgaactgc gccgcaagat gcaccagctc tacgaattct
     2401 ggctgagggt caacgaacgc tgccggccga ccggaacctg aggctcaggc aagccccgcc
     2461 gcgatccggc gcagggcctg ttcgaacacc tccggcggct gtccgccctg caccaggtgc
     2521 ctgtcgttga cgatgaccgc aggaacggcg tggatgccgc ggtccaggta gaaccgctgg
     2581 cgctcgcgca cctcggccgc gtaccggccg ctttccagga cgtcgcgcgc acccgccggg
     2641 tcgaggccgg cccggccggc ggcctgtacc agcacgtcac ggtccgcggg gctgagcccg
     2701 tgggtgaagt aggcctggag caaggccttc ttcagcgcgt gctggcggcc cggtgcggcc
     2761 agcgcggccc agtgcagcag gcgatgggca tcgaaggtgt tgtagatgcg cccgcgccct
     2821 tccttgcgga aatcgaaacc caccgccgca ccgcgttcgc gaatggcgtc ccgcgcctgc
     2881 gcctgctgct ggggcgtgct gcggtatttc tgcgtcagat gctcggtgat gtcctgcccg
     2941 cccggcggca tggccgggtt gagttcgaac ggctggaagt gcaggtcggc ccgcacttcg
     3001 ccgtgcaggc gctcgagcgc cctttccagc gacgccagcc cgatggcgca ccaggggcag
     3061 gacacgtcgg atacgaaatc gatcttgagc gcggtcgtca tcaggcccat cctagcccca
     3121 catgccgagg tcgcacccga gcgcgtggct cagcacgacg gccgggccgc tgctcagctt
     3181 gacgaaattg agttgacctg aacgcgcgcg gtaggagccg gcctacgtcg cctgtagcgg
     3241 atcaatgtcc tgaaggcacc ttcctgagca ggtacttctg gacctcatgg acctggtggc
     3301 cggcatgatg aaccgcttcg cgcagctggg ccatgctcac gttgaggacg cggcaccagt
     3361 aggcgacgtc catctcgcgc gtcaggtcga tgcgctgggc cggcggggtt gacgtattgc
     3421 tgtccatgtg cggcctcctt agggggtagg agtcagagta tggcgcgccg cgccgagttc
     3481 cgcgacagtt cctgcaggcg ctcgacgtca accggcttgg tccagtgctc gtcgaatccg
     3541 gcttgcgccg ccacctgctt gtcctgctgc tggccccagc cggtcagggc gatcagcctc
     3601 ggctgcccga tgccctccag ccgccgcacg cgccgtgcca cttcgtagcc gttgatgtcg
     3661 ggcaggccga tgtcgagcag caccacgtcg ggcccgaact gggcgacgcg ctcaacggcc
     3721 gagctgccgt tcagggccac ggccacctca ttgccgagca tgcgcagcag ctcggccagc
     3781 gtctcggcag cgtccttgtt atcgtccacc accaggatgc ggcaggcccg gggtgcctgg
     3841 gcgtgcgcct cccccggcgc ggcgcctgcc tggggcactg gggcgtggga ttgtgcgtcg
     3901 tgaggggggt ggtcgttgcg gtcgtgcatg gtgatcggcg cgaagggata tttcgcaagg
     3961 gtaatcgcat tcgcccctcc ctgggtgccc gccgcggccg ggccggccgg catcgggtca
     4021 gacagcgagc gtcggggcgc cgcgcgcgcg acgcagggcg acccaggtcc acgccgcgca
     4081 agccagatcg atcaggccga acagcagcag cacgggtggg gcgtagccca gcagcacgaa
     4141 gacggcgaaa aagaagatga ccgatgcgcg catcggcacc gaccagcgga tgaactggtg
     4201 gagttccgac aggctggcca ggatgtcgta cgcgccgagg atcagcacca gcatgccggt
     4261 cacgcgaatc cacacctcga ctgtcggcgc gacgccaaaa agctgcagca cgatattggg
     4321 aaagacgatg aggagcatgc cgagcaccag caggtagcag ccgaatgcga acagggtctt
     4381 ggcgggatag ctcatggcgc ggtattcgcc cgatcagggc aacgatgcgg ggatggctgc
     4441 gacgggtgac acgacgtcgc cgttttgcgc gcgctgccgc agcgcgtgct ccatcacgac
     4501 cagggccagc atggcctcgg caatcggcgc ggcgcggatg ccgacgcacg ggtcgtggcg
     4561 tcccttggtg acgacctggg tggcttcacc cttgaggttg atcgtttcgc gcgggctcag
     4621 gatcgagctg gtgggcttta tcgcgatcga gacctcgaga tcctgcccgg tgctgatgcc
     4681 gcccagcaca ccgcccgcgt tgttggtcct gaagccctgc ggcgtgggtg aatcgccatg
     4741 ggtggtgccg cgctgcgtca cgctcgcgaa gccggcgccg atctcgacgc ccttgacggc
     4801 gttgatgccc atcatcgccc atgcgatgtc tgcgtcgagc ttgtcgaaca agggctcacc
     4861 caggccgacc gggacgttcg acgcggtgac gcgaatgcgc gccccgcatg aatcgccggc
     4921 cttgcgcagg ctgtccatgt agctttccag ctgcgccacg tccgcaaccg gtgcgaagaa
     4981 cgggttgttg ggaacatggt cccagctctc gaacgctatg gcgatatcgc ccatctgctg
     5041 catgcaggcg cgaaacaccg tgccgtattt ctctcgcagc cacttcttcg cgacggcgcc
     5101 ggcagccacc atgggcgccg tcaggcgggc cgaggcgcgg ccgccgccgc gcgggtcgcg
     5161 cagcccgtat ttttgcaggt aggtgtagtc ggcgtggccc gggcgaaagg tctgcgcgat
     5221 ctccgaatag tccttgctgc gctggtcggt gttgcggatc agcaggccga tcggcgtgcc
     5281 cgtggtcttg ccttcgtgca cccccgacag gatctccacc cggtccgcct cctggcgctg
     5341 ggtcacgtgc cggctggtgc ccgggcggcg ccggtccaga tcgggctgga tgtcggcttc
     5401 gccgagcgcc atgccgggcg ggcagccgtc gatcacgcag ccgatggccg gcccgtgcga
     5461 ttcgccaaag ttggtgaccg caaaaagtgt tccgaaggtg ttgccgctca ttccaaaaga
     5521 tctcccaaac cattctcacc ctctccctcc gggagagggt tggggtgagg gctggcggat
     5581 tatcccagag cggcggactg ccgcagttgt gcctagagct gcggcccggg cgactgctcc
     5641 tcgacgcctc gcgaatcggt gatataggcc ttgagcatgc gattttcgaa gttctggctg
     5701 tccaccaccg ccctggccac atcgtagaaa ctgatcacgc ccatcagcac gcggcgctcc
     5761 atcaccggca tgtaacgggc atggctttcc agcatgaggc ggcgcacttc atcgagctcg
     5821 gtgtcggggg cgcacgtgag cgggtggtcc tccatcgccg agcgcaccac cgaatcaccc
     5881 acgaggccac cgttcctgac gatgcacagg atcacctcgc ggaacgtcag catgcccacc
     5941 agctcgccgc gctccatcac caccacggag ccgatgtcct tgtcggccat cagtttcatc
     6001 gcctgcgcca ggggttcgtc gggcgtgacg gtgtacaggg tgccgccctt gacccgcagg
     6061 atgtcgctga ccttcatttt cgtctcctct gtggcaccgg tgagcttccg gtcaagtgaa
     6121 tatagcccac aatcggggcc agttccatag cgcattgatc gaggaggcat agcaatgccc
     6181 ggttactccg acccaggctt cgacactctg gccctgcacg ccggggccgc ccccgacccg
     6241 gcgaccggcg cgcgggccgt gccgatccac ctgaccacct cgttcgtgtt cgattccagc
     6301 gaccacgcgg cggccttgtt caacctggag cagtccggcc acgtctattc gcgcatcagc
     6361 aacccgacca atgcggtgct ggaacagagg gtggccgcgc tggaaggcgg cgtcggcgcc
     6421 atttcgaccg ccagcggcca ggcggctttg cacctgagca ttgcgacgat catgggcgcg
     6481 ggatcccaca tcgtggccag cacggccctc tacggcggct cgcagaacct gctgcattac
     6541 acgctgcggc gcttcggcat cgccaccacg ttcgtcaagc cgggcgatat cgacggctgg
     6601 cgcgccgcga tccggccgga cacgaagctg ctgttcggcg agacggtggg caacccgggc
     6661 ctcgaggtcc tcgatatccc gcgggtcgcc gcgatcgcgc acgaggccgc agtgcccctg
     6721 ctcgtcgatt ccactcttac ctcgccttac ctgatgaggc cgctggcgct aggcgccgac
     6781 ctggtgtacc actcggccac caagttcctc tcgggccacg gcacggtgat cggcggcgtg
     6841 gtggtggacg gcggcagctt cgactgggag cgttccggca aattccccga gctcaccgcg
     6901 ccctacgacg gtttccatga catggtgttc agcgaggagt cgacggtcgg cgccttcatg
     6961 ctgcgcgccc gccgcgaagg gctgcgcgac ttcggtgcct gcatgagccc gcacacggcg
     7021 tggctgatcc tgcagggcat cgagacgctg ccgctgcgca tggagcgcca tatgcacaac
     7081 acgcgcaagg tggtggaatt cctggcggcc catccattcg tcgcgcacgt ggggcatccg
     7141 atgctggaat cgcaccccag ccacgcgctg gcgcgagaac tgctgcccaa gggagccggc
     7201 tcggtcttca gcttcgacat gaagggcaac cgcgagcagg gcaagaagtt catcgaggcg
     7261 ctcaagctgt tcagccacct ggcgaacgtg ggcgactgcc gctcgctcgt gatccatccg
     7321 gccagcacga cgcatttccg aatgagcgac gaggcgctgg ccgccggcgg catcactcag
     7381 ggaacgatcc gcctctcgat cgggctggag gaccccgacg acctgatcga cgacctgaag
     7441 cgggcgttga aggcggcgga gaaggcatga gcatcgtcat tgcggcctcg agccgcaatc
     7501 catggccgct ggcgcaccac catggatgcc cccggatcaa gtccggggca ggctcgtcaa
     7561 gcccggcatg acagacttca ggaggcaggg atgtatttga aggtc
//
LOCUS       DASWIY010000130         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1936100, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000130 DASWIY010000000
VERSION     DASWIY010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936100"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VGA59_05085"
     CDS             complement(<1..892)
                     /locus_tag="VGA59_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX9719086.1"
                     /translation="MKRWIVFVLVLLLHAAAWAHKPSDSYLTLRGAPGTDDIAVRWDI
                     ALRDLDYVLELDRDGNGALTWGEVRQRADEIARYATGHLELATGDKGDKACRWDVSGP
                     LMLDKHSDGTYAVLSLVAHCESLAGGLKARYSLLFDVDPSHRGLVQWIAPGSQGSQAL
                     LFGTESAEQALALAPQGAWPTLRQYTLDGTWHIWIGYDHILFLLALLLPSVLIHRRKR
                     WEAVSRPGPAIREVVKVVTAFTLAHSITLSLAALQVISLPSRLVESAIAASVVLAALN
                     NLRGTIENRRWVVAFVFGLIH"
     gene            complement(889..2079)
                     /locus_tag="VGA59_05090"
     CDS             complement(889..2079)
                     /locus_tag="VGA59_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719087.1"
                     /translation="MPEKPTTTLAAGLLLACAVATAAPYTPANDNVVVERLPAGATDP
                     SVRRVDSLRKQLAARPNDTGLRLEIARRYFDLAMAQGDPRYVGYASAAIGPLAQSATG
                     DARYWLVRGLIQQYSHDFAGALQSLEKASELDPQSPDPIAWRAAVNMVQARYREALSE
                     CTQLVPLANPLHAQGCTAYVLASTGQLAPAYELFAKGLAAAGDIPPELTLWAQTRLAE
                     MAIRLQRFGEAEAHFKEALRQGVTDQFLLGAYADFLLLQKRPAEVVTLLADWERSDIL
                     LLRLALAGRALNDPRSAGWAGQLRDRFAAAAQRGDRLHEQEAARFELDIEGKPDRALA
                     LAVRNYQAQKEPRDAEVLLRAALAANQPKAAQPALDWLRTNQYEDPALARLAGELAAK
                     GATR"
BASE COUNT          372 a          788 c          802 g          366 t
ORIGIN      
        1 catggatcag cccgaagacg aaggccacga cccaccggcg gttttcgatg gtgccgcgca
       61 ggttgttcag agccgccagc acgaccgaag cggcgatcgc cgactccacc agccgcgagg
      121 gcaggctgat tacctgcaag gcggccaggc tcagcgtgat ggaatgcgcc agcgtgaacg
      181 cggtcaccac tttgacgact tccctgatcg ccggtccggg gcgcgatacg gcttcccacc
      241 gctttcgccg atggatcagg acggacggca gcagcagcgc cagcaggaac aggatatggt
      301 cgtagccgat ccagatatgc caggtgccgt ccagtgtgta ctggcgcaac gtcggccagg
      361 ccccctgcgg cgccagggcg agcgcctgtt cggccgactc ggtgccgaac aagagcgcct
      421 gcgatccctg gctgcctggc gcgatccatt gcaccaggcc ccgatgcgaa gggtcgacgt
      481 cgaagagcag cgaatagcgc gccttcaggc ccccggcgag gctctcgcaa tgcgcgacca
      541 gcgacagcac ggcgtaggtc ccgtcgctgt gcttgtcgag catcaagggg ccgctcacgt
      601 cccagcggca agccttgtcg cccttgtcgc cggtcgccag ctcgagatgg ccggtggcgt
      661 agcgggcgat ctcgtccgcc cgctggcgca cctcgcccca ggtcagcgcg ccgttgccgt
      721 cgcggtccag ctccagcacg tagtcgaggt cgcgcaaggc aatgtcccag cgcacggcga
      781 tgtcgtcggt gcccggcgct ccgcgcagcg tgagataact gtcgctgggc ttgtgggccc
      841 aggcggctgc gtgcagaagc agcaccagca cgaacacaat ccagcgcttc atcgcgtcgc
      901 gcctttggcg gccagctcgc ccgccagccg cgccagggcc gggtcctcgt actggttggt
      961 gcgcagccaa tccagcgcgg gctgggccgc tttgggctgg ttggccgcca gggccgcgcg
     1021 caggaggact tcggcgtcgc gcggctcttt ttgcgcctgg tagttgcgca ccgccagtgc
     1081 cagggcccgg tccggcttgc cctcgatgtc gagctcgaaa cgcgctgcct cctgttcgtg
     1141 caatcggtcg ccccgctgcg cggccgccgc gaaacgatcg cgcagctggc cggcccagcc
     1201 cgctgagcgc ggatcgttca gcgcgcgccc ggccagcgcc aggcgcaaca acaggatgtc
     1261 ggagcgttcc caatcggcca gcaaggtcac gacttcggcc ggccgttttt gcagcagcag
     1321 aaaatccgca taggcgccca gcaggaactg atcggtcacg ccctggcgca gcgcctcctt
     1381 gaaatgcgcc tcggcctcgc cgaatcgctg cagccgtatc gccatctctg ccaggcgtgt
     1441 ttgcgcccac agggtgagtt ccggcgggat gtcaccggcg gcggccagcc ccttggcgaa
     1501 caattcatag gccggcgcca attggcccgt gctggcaagg acataggccg tgcagccttg
     1561 cgcgtgcagt ggattcgcca gcggaaccag ctgtgtgcac tcggacaacg cctcgcgata
     1621 acgcgcctgc accatgttga ccgccgcgcg ccaggcgatc ggatcgggcg actggggatc
     1681 cagctcgctg gccttctcga ggctttgcaa agcgccggca aagtcatggc tgtactgctg
     1741 gatcaaaccg cggaccaacc agtagcgggc gtcgccggtc gcggactgcg ccagcggacc
     1801 gatggcggcc gacgcgtagc ccacatagcg gggatcgccc tgcgccatcg ccaggtcgaa
     1861 gtagcggcgg gcgatctcca gccgcaggcc cgtatcgttg ggccgcgcgg cgagctgctt
     1921 gcgcagcgaa tccacgcggc gtacggaagg gtccgtcgca ccagcgggca ggcgctcgac
     1981 gacgacgttg tcattggcgg gggtgtaggg ggcggctgtc gcaacggcgc aggcgaggag
     2041 cagtccggcg gcaagtgttg ttgttggttt ttctggcatc gacgcgagtc taccggtcgc
     2101 gccgacatgg attgcggggc cgggccggag cgtgatgcct cggcacgtgc atagcgcagg
     2161 cccggcattg ggctcgctgc actggacagc tgcgcgcccc gggcgttccg cgagcccagc
     2221 ccgcaatgac gcaactggcg gagttgtcat gccggacttg atccggcatc catggaacgc
     2281 gggtcagccg gcaacgataa cgctatttcg tgccgaagat ccggtcgc
//
LOCUS       DASWIY010000131         4577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_71255, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000131 DASWIY010000000
VERSION     DASWIY010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71255"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /gene="dctP"
                     /locus_tag="VGA59_05095"
     CDS             complement(<1..520)
                     /gene="dctP"
                     /locus_tag="VGA59_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9719088.1"
                     /translation="MKLSQLVLGVTLAFGFVAGAAAQTTMKISISTTQNSHQGIAIDT
                     FAREVEKRTNGRYKIQTFYNGALGGERESIEAVQLGTQALAFSSTGPVPNFVPETKIL
                     DVPFLFRDKAHARAVLDGPIGQEMLTKFDAKGFKALAWAENGFRHMTNSKRDVKLPGD
                     LKGLKMRTMENPV"
     gene            complement(637..2319)
                     /locus_tag="VGA59_05100"
     CDS             complement(637..2319)
                     /locus_tag="VGA59_05100"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX9719089.1"
                     /translation="MTHEEILAQYGPREAMEYDVVVVGGGPAGLSAAIRLKQVAAEHG
                     KEVSVVVLEKGSEPGAHILSGAIMDPRALYELFPDWEKMGAPLTTPVTEDIFLFTSEK
                     GASRTPNFMLPACFQNHGTYVLSLASLTRWLAQQAEGLGVEIFPGFAAAEVLYNEDGS
                     VKGVATGNMGIGKDGEPTGSFQLGMELHAKYTIFAEGSRGHLGRQLIERFKLDEGRDP
                     QSYSIGMKELWEIDPSRHKPGLVLHSAGWPLEGDTYGGSFMYHMEGNQMAVGFVVGLD
                     YKNPWLSPFDEFQRWKTHPNIRWYFEGPTGAKRLGYGARALTVGGLLSLPKTVFPGGA
                     LVGCEAGFLNSSRIKGSHGAIKTGMLAANAAYEAIAAGRRHDELAAYPAAFEKSWLYD
                     ELNKSRNFKQWFKKGRSVATFMTGIEQFVLRGHIPWTIHREKPDHVYLKPASECKPIV
                     YPKPDGKLTFDRLSSVFISNTNHSEDQPAHLTLKDDSVPVQVNLAKYAGPEGRYCPAG
                     VYEFVKDENNQDRLQINASNCVHCKTCDIKDPTQNIVWVTPEGGGGPNYTNM"
     gene            2457..3239
                     /locus_tag="VGA59_05105"
     CDS             2457..3239
                     /locus_tag="VGA59_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9719090.1"
                     /translation="MSYSIDLSGRVAFVTGASSGLGAQFARALARAGAGVVLASRRIE
                     RLKDLRAQIEAEGEDAHVVALDVTSIDSIKSAVAHAETEMGSIDILVNNSGVSTTQRI
                     QDVSEEDYDFIFDTNVKGAFFVAQEVGKRMLARARGAAPGTYTGGRIINIASMAGLKV
                     LPQIGAYAISKAAVVHMTKAMALEWGRFGINVNAICPGYIDTEINHHHWSTEQGQKLM
                     QMLPRKRVGKPEDLDALIVLLASGQSHFINGAVIAADDGFAV"
     gene            3258..3677
                     /locus_tag="VGA59_05110"
     CDS             3258..3677
                     /locus_tag="VGA59_05110"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9719091.1"
                     /translation="MKIEIPEKKKLVHEMRIPIRWGDMDAMNHVNNTIYFRYLEIARI
                     DWMSSIGCQPDPQGEGPVIVNAFCSFYKQLEYPGEVLLKMYVSDPGRSTFESWGTMER
                     ADDPGVIYAAGGATTVWVNFPAQKSAPLPDSMRRHLE"
     gene            complement(3763..4335)
                     /locus_tag="VGA59_05115"
     CDS             complement(3763..4335)
                     /locus_tag="VGA59_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEX9719092.1"
                     /translation="MNRYPAPELKDLPDDIKARVLEVQEKAGFVPNVFLALARRPAEW
                     RAFFAYHDALMLKEEGSLTKGDREMIVTTTSAANQCLYCVVAHGAILRIYEKKPMVAD
                     QVAVNYRKADITARQRAMLDFAMKVCLRSHEIDEADFPPLHAHGFSDEDIWDIAAITA
                     FFGLSNRMASFSDMAPNPEFYLMGRVPKQK"
     gene            4395..>4577
                     /locus_tag="VGA59_05120"
     CDS             4395..>4577
                     /locus_tag="VGA59_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEX9719093.1"
                     /translation="MDDAFDKGLTTRKQVMGEEYVTRAFANATDFTRPIQEHITRAAW
                     GDVWQREGLDRRTRSLV"
BASE COUNT          813 a         1420 c         1475 g          869 t
ORIGIN      
        1 gcaccggatt ttccatggtg cgcatcttca ggcccttgag gtctccgggc agcttcacgt
       61 cgcgcttgct gttggtcatg tggcgaaagc cgttctcggc ccaggccagt gccttgaagc
      121 ccttggcgtc gaacttcgtc agcatctcct ggccgatcgg gccgtcgagc acggcgcgcg
      181 catgcgcctt gtcgcgaaag aggaacggca cgtccaggat cttggtctcc ggcacgaaat
      241 tgggcaccgg cccggtggaa ctgaaggcca acgcctgggt gcccagctgc accgcctcga
      301 tcgattcgcg ctcgccaccg agggcgccgt tgtagaaggt ctggatcttg taccggccgt
      361 tggtgcgctt ttcgacttca cgcgcgaagg tgtcgatggc gatcccctgg tgggagttct
      421 gcgtcgtcga gatgctgatc ttcatggtgg tttgcgccgc cgcgccggcc acgaagccga
      481 aggcgagcgt gacgcccagg accagttgcg aaagtttcac tgtcagtctc caaatcagaa
      541 ataagaaccc ccgggattct gccagaagtt taaaagttgt catacaactt ttttatgcgg
      601 tcgtcgccct gatccggcat ccctgcaccc atgaaatcac atgttcgtgt agttcggccc
      661 gccgccgccc tcgggggtga cccagacgat gttctgcgtc gggtccttga tgtcgcaggt
      721 cttgcaatgc acgcagttcg acgcgttgat ctgcaggcgg tcctggttgt tctcgtcctt
      781 gacgaactcg tacaccccgg ccgggcaata acggccctcg ggccccgcgt acttggcgag
      841 gttcacctgc acgggaacag aatcgtcctt caacgtgagg tgagcgggct ggtcttcgga
      901 atggttggta ttcgagatga acaccgacga caggcggtcg aacgtgagct tgccgtccgg
      961 cttgggatac acgatcggct tgcactcgga cgcgggcttc aggtagacgt ggtcgggctt
     1021 ttcgcggtgg atggtccacg ggatgtgccc gcgcaaaacg aactgttcga tccccgtcat
     1081 gaaagtggcg acggagcggc ccttcttgaa ccactgcttg aagttgcgcg acttgttcag
     1141 ctcgtcgtag agccaggact tctcgaacgc ggccggatag gccgcgagct cgtcatggcg
     1201 gcggcccgcg gcaatcgcct cgtacgcggc gttggcggcc agcatcccgg tcttgatggc
     1261 gccgtggctg cccttgatgc gcgacgaatt caggaaaccg gcctcgcagc ccaccagcgc
     1321 gccgccgggg aacaccgtct tgggtagcga cagcaggccg cccacggtca gcgcgcgagc
     1381 gccgtagccc aggcgcttgg ccccggtcgg gccttcgaaa taccagcgga tgttcgggtg
     1441 cgtcttccag cgctggaact cgtcgaacgg gctgagccag gggttcttgt aatccaggcc
     1501 gacgacgaac cccaccgcca tctggttgcc ctccatgtgg tacatgaagg agccgccgta
     1561 ggtatcgcct tccaggggcc agccggcact gtgcagcacc aggccgggct tgtggcgcga
     1621 cggatcgatc tcccacagct ctttcattcc gatgctgtag ctctgcggat cgcgtccttc
     1681 gtccagcttg aagcgctcga tcagctgccg gcccaggtgg ccacgcgagc cttccgcgaa
     1741 gatggtgtac ttggcgtgca gttccatgcc gagctggaaa ctcccggtgg gctcgccgtc
     1801 cttgccgatc cccatgttgc ccgtggcgac gcccttgacc gaaccgtcct cgttgtaaag
     1861 cacttcggcc gcggcgaagc ccgggaagat ctcgacgccc aggccttcgg cctgctgggc
     1921 cagccagcgc gtgaggctgg ccaggctcag gacgtaggtg ccatgattct ggaagcacgc
     1981 gggcagcatg aagttgggcg tgcggctggc gcccttctcg ctggtgaaca ggaagatgtc
     2041 ctcggtgacg ggcgtggtca ggggggcgcc catcttttcc cagtccggaa acagctcgta
     2101 aagtgcgcgc ggatccatga tggcacccga gaggatgtgg gcgccgggct ccgaaccctt
     2161 ctccagcacc accaccgaca cctccttgcc gtgctcggcc gccacctgct tcaggcggat
     2221 ggccgccgac agcccggccg ggccgccgcc caccacgacc acgtcgtatt ccatcgcctc
     2281 gcgcggtccg tactgcgcca gtatttcttc gtgggtcata gggctctcgt tgataatggt
     2341 ttgataaacg acgcgcgacg gcactgtgcc gcggatggga ttgattctat tgccgcgagg
     2401 ccaaaaaatc gaacgatcgt tcttattccg actcctattt taagcaggag actcttgtga
     2461 gttacagcat cgacctatcg ggacgcgtcg ccttcgtgac cggcgcttcc agcggcttgg
     2521 gggcgcaatt cgcgcgagcg ctggcgcgcg ccggggccgg cgtggtcctc gcgagccgcc
     2581 gcatcgagcg gctcaaggac ctgcgcgccc agatcgaagc cgaaggcgaa gacgcgcacg
     2641 tggtggccct ggacgtgacc tcgatcgaca gcatcaagtc ggcggtcgca catgccgaga
     2701 cggagatggg gtcgatcgac atcctcgtga acaactccgg tgtgagcacc acccagcgca
     2761 tccaggacgt gagcgaagag gactacgact tcatcttcga caccaacgtc aagggcgcgt
     2821 tcttcgtggc gcaggaagtg ggcaagcgca tgctggcgcg cgcgcgcggc gccgcgcccg
     2881 gcacttatac cggcgggcga atcatcaaca tcgcctccat ggccggcctc aaggtgctgc
     2941 cgcagatcgg cgcctacgcc atcagcaagg cggcagtggt gcatatgacg aaggcgatgg
     3001 cgctcgagtg ggggcgcttc ggcatcaatg tcaatgcgat ctgcccgggc tacatcgaca
     3061 ccgagatcaa tcatcaccac tggtccaccg agcagggcca gaagctgatg cagatgctgc
     3121 cgcgcaagcg cgtgggcaag ccggaggacc tggacgcact gatcgtcctt ctggccagcg
     3181 gacagagcca tttcatcaat ggagcggtga tcgcggccga cgacggcttc gccgtctgaa
     3241 cacgcgttga caaacgaatg aaaatcgaga tccccgaaaa gaagaaactg gttcacgaga
     3301 tgcgcatccc gattcgctgg ggcgacatgg atgcgatgaa ccacgtcaac aacacgattt
     3361 atttccgcta cctggagatc gcgcgcatcg actggatgag ttccattggg tgccagcccg
     3421 atccgcaggg tgagggaccg gtgatcgtca acgcgttctg cagcttctac aagcagctcg
     3481 aatacccggg cgaggtgctg ctgaagatgt atgtgagcga ccccggccgc agcaccttcg
     3541 aatcctgggg cacgatggag cgagccgacg atcccggcgt gatctatgcg gccggcggcg
     3601 cgaccacggt gtgggtcaac ttcccggcgc agaaatccgc gccgctgcct gacagcatgc
     3661 gacgccacct ggagtgatgg gtcgtcgccc tgtggttccg ggatgacaac cccatttctg
     3721 tcattgcggg cttgacccgc aatccaggag ttctcgcttt gtctatttct gcttgggcac
     3781 ccgtcccatc aggtagaact ccgggttggg cgccatgtcg gaaaagctcg ccatccggtt
     3841 cgacaggccg aagaaagccg tgatcgcggc gatgtcccag atgtcctcgt cggaaaagcc
     3901 gtgcgcgtgc agcggcggga agtcggcctc gtcgatctcg tgcgagcgca ggcagacctt
     3961 catcgcgaag tcgagcatcg cgcgctggcg cgccgtgatg tccgccttgc ggtagttcac
     4021 cgcgacctgg tcggccacca tcggtttttt ctcgtagatg cgcaggatcg cgccgtgggc
     4081 caccacgcag tacaggcact gattggccgc gctggtggtg gtgacgatca tctcgcggtc
     4141 gcctttggtc agcgaccctt cctccttgag catcaacgcg tcgtggtagg cgaagaaggc
     4201 gcgccactcg gcgggccggc gcgccagcgc gaggaaaacg ttgggcacga agcccgcctt
     4261 ctcctgtact tcgagcacgc gcgccttgat atcgtcgggc agatccttga gttcgggggc
     4321 ggggtagcgg ttcatggcat ctctcctatg ggcttgcgca gattatgctg tgcgcctgta
     4381 tggaaggagc aagcatggat gacgcattcg acaagggctt gacgacgcgc aagcaggtca
     4441 tgggcgagga atatgtgacc cgggctttcg ccaacgccac ggatttcacg cggccgatcc
     4501 aggagcacat cacccgtgcc gcgtggggcg acgtgtggca gcgcgaggga ctggaccgca
     4561 ggacccgcag cctggtc
//
LOCUS       DASWIY010000132         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1938455, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000132 DASWIY010000000
VERSION     DASWIY010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938455"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VGA59_05125"
     CDS             complement(<1..1261)
                     /locus_tag="VGA59_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9719094.1"
                     /translation="MTYQIPLRDFQFVLHELLQVPSMMEACGQPEFDVATIDQVLTAA
                     GQFASEVIAPLNAAGDEHGCSMPGPGVVQTPPGFKEAYDEFSRNGWHGLACSPEFGGQ
                     GFPSVVDTAVTEMFGGANMAWSAYPGMSHATYVNVAANGSAEQKALYLPKIASGEWAG
                     TMCLTEPHAGTDLGLLRTRAIPQPDGTYRITGTKIFISGGEQDLTENVMHLVLARMPD
                     SPQGVKGISLFIVPKFIPDATGAVGPRNAVTCGSIEHKLGIHGNSTCTMNFDGATGWL
                     LGEADRGLAGMFVQMNYMRVLVGVIATGVMEAAYQKALAYARERLQGRVAGTRASNQS
                     ADPIAGHADVRRMLLTQKANIEGARALALWASQLSDLQHLHPDAGRRAEAAELLGLLT
                     PVVKAMASDLGVESTLLAIQVFGGHGYI"
     gene            complement(1278..>1917)
                     /locus_tag="VGA59_05130"
     CDS             complement(1278..>1917)
                     /locus_tag="VGA59_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX9719095.1"
                     /translation="QEKRLFDDEIVPITTSMTVADKDTGAVTCRDVRLDRDEGNRPDT
                     TLEGLAKLKPVVEGGVITAGNSSQLSDGASACVLVERTLAERRGLKPLAIYRGIAVAG
                     LAPEEMGIGPVFAVPKLLKTHGLKVSDIGLWELNEAFACQVIYSRDQLGIDPDILNVN
                     GGGISIGHPYGMTGSRLVGHALIEGKRRGARYVVVTMCVGGGMGAAGLFEVA"
BASE COUNT          318 a          652 c          606 g          341 t
ORIGIN      
        1 ggatgtagcc gtggccgccg aacacctgta tcgcgagcaa cgtggactcg accccgagat
       61 cactggccat ggccttgacg acgggagtca gcaggcccag cagctcggca gcttcagcgc
      121 gtctcccggc gtcaggatga aggtgctgca gatcgctgag ctgcgaagcc cacaaggcga
      181 gtgcgcgggc gccctcgatg ttggccttct gcgtcagcag catgcgccgc acatcggcat
      241 ggccggcaat cgggtccgcg ctctgattcg acgccctcgt gcccgccaca cgcccctgca
      301 ggcgctccct ggcataagcc agagccttct gatacgcggc ttccatcacg ccggtggcga
      361 tgacgccgac cagcacccgc atgtagttca tctgcacgaa catgccggcc aatcccctgt
      421 ccgcctcccc gagcagccag cctgtcgccc catcgaagtt catggtgcag gtcgagttgc
      481 cgtggatgcc cagcttgtgt tcgatggagc cgcaggtcac ggcattgcgc ggaccgacgg
      541 cgccggttgc gtccgggatg aacttgggca cgatgaagag cgagatgccc ttgacgccct
      601 gcggcgaatc gggcatccgc gccagcacga ggtgcatcac gttttcggtc aggtcctgtt
      661 cgccgccgga gatgaagatc ttggtgcccg tgatgcggta cgtcccgtcg ggttgcggga
      721 tggcgcgggt tcgaagcagg ccgagatccg tgcctgcgtg cggttctgtc aggcacatgg
      781 tgccggccca ctcgccggaa gcgatcttcg gcagatagag cgccttctgc tcggctgagc
      841 cattggcggc cacgttgacg taagtcgcat gcgacatgcc gggataggcc gaccacgcca
      901 tgttggcgcc gccgaacatt tccgtgacgg cggtgtcgac caccgaggga aagccctggc
      961 cgccgaactc cgggctgcac gccagcccat gccagccgtt cctgctgaac tcgtcgtacg
     1021 cctccttgaa accaggcggt gtttgcacca cgccgggccc cggcatgctg cacccgtgct
     1081 cgtcaccggc cgcgttcagc ggcgcgatca cctcggacgc aaactggccg gcagcggtca
     1141 gcacctggtc gatcgtggcc acgtcgaact ccggctgccc gcacgcctcc atcatcgaag
     1201 gaacctgcag caactcgtgc agaacgaact ggaaatcgcg caaggggatc tggtaggtca
     1261 tcgggctgct tctcgattca ggcgacttcg aacaggcctg cggcccccat gccgccgccg
     1321 acacacatcg tgaccacaac gtagcgcgcg ccgcgccgct tgccttcgat gagtgcgtgg
     1381 cccaccagtc tcgaacccgt catcccgtac ggatggccga tgctgatgcc tccgccgttc
     1441 acgttgagga tgtcggggtc gatgccgagt tgatcgcgcg agtaaatcac ttggcaggcg
     1501 aaggcttcat tcaattccca caagccgatg tcgctcacct tcagaccatg ggtcttcagg
     1561 agcttgggca ccgcgaacac cggtccgatg cccatctcct ccggcgccaa tccggcgacg
     1621 gcgatgccgc ggtagatagc cagcggcttc agcccgcggc gttcggccaa tgtgcgctcg
     1681 accagcacgc atgcggatgc gccgtccgag agctggctgg agttaccggc cgtgatcacg
     1741 ccgccctcga ccaccggctt gagcttggcg aggccttcca gtgtggtgtc gggccggttg
     1801 ccttcgtcac ggtccagccg cacgtcccgg caagtgacgg cgcccgtgtc cttgtcggcc
     1861 acggtcatcg acgtggtgat cggcacgatc tcgtcatcga acaggcgctt ctcctgg
//
LOCUS       DASWIY010000133         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3392280, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000133 DASWIY010000000
VERSION     DASWIY010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3392280"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VGA59_05135"
     CDS             <1..843
                     /locus_tag="VGA59_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX9719096.1"
                     /translation="AWRTPVEGARSDHAAISPDGKTLLVSASTARKVHAIDTATGRIV
                     GGFESGDQPHENNYSKDGRRIYHASIGKVYVPTTAAWLDGIKGDRWFQVVDAQTYQVL
                     KRIDMGEKLAEFGRPWVDKAVRPMAVSPDEKFVYLQISFFHGFFEYDLAQDKITRGVD
                     LPVPQEIADLSYWKYQLNSAHHGLAINGEGTKLCVAATMSGYAAIVDRETLKPTIITL
                     SETPLGAKPYWATESADGRHCYISVSEQDRVSVISFDEAREVASVPVGDHPQRVRTGK
                     LLLP"
     gene            complement(930..1148)
                     /locus_tag="VGA59_05140"
     CDS             complement(930..1148)
                     /locus_tag="VGA59_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719097.1"
                     /translation="MSGAKIAGILLLIAGALALVYGGFSYTSDTHRADLGPLHMTVKE
                     KDYLNIPVWAGIGALVVGGMLLVLRQKS"
     gene            complement(1145..1294)
                     /locus_tag="VGA59_05145"
     CDS             complement(1145..1294)
                     /locus_tag="VGA59_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX9719098.1"
                     /translation="MLETIAILLLVLWAVGFVSSYTMGGLIHLLLVVAVVVVLVRVIQ
                     GRRVV"
     gene            1689..>2092
                     /locus_tag="VGA59_05150"
     CDS             1689..>2092
                     /locus_tag="VGA59_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9719099.1"
                     /translation="MPVAKVNVVNQLIRDLPQKLRARLLARCEQVELVFGTILCEANV
                     PFTHVYFPLRGFISLVATVDDHEPLEMGLIGNEGMLGATITLGVDVVPLRGVVQGSGS
                     ALRMSAARFRAELRDSPDLRRMVNRYLYVLMA"
BASE COUNT          376 a          692 c          704 g          320 t
ORIGIN      
        1 gcctggcgca cgccggtcga aggcgcccgc tccgaccatg cggcgatctc gcccgacggc
       61 aagaccttgc tggtctcggc ctcgaccgcc cgcaaggtgc acgcgatcga cacggccacg
      121 ggacgcatcg tcggcgggtt cgagagcggc gaccagccgc acgagaacaa ctactcgaag
      181 gacggccggc gcatctacca cgccagcatc ggcaaggttt acgttccgac cacggcggcc
      241 tggctcgacg gcatcaaggg cgaccgctgg ttccaggtcg tcgacgcgca gacctaccag
      301 gtgctcaagc gcatcgacat gggcgagaag ctcgccgagt tcggccggcc ctgggtcgac
      361 aaggccgtgc ggccgatggc ggtgtccccc gacgagaagt tcgtctacct gcagatctcg
      421 ttcttccacg gcttcttcga gtacgacctg gcgcaggaca agatcacgcg cggggtcgac
      481 ctgccggtgc cgcaggagat cgccgacctg tcgtactgga agtaccagct caactcggcg
      541 caccacggcc tggcgatcaa tggcgaaggc acgaagctgt gcgtcgccgc gaccatgtcg
      601 ggctatgccg cgatcgtgga ccgggaaacg ctgaagccca cgatcatcac gctgtcggaa
      661 acgccgctgg gcgccaagcc gtactgggcg accgagagcg ccgacggcag gcactgctac
      721 atctcggtca gcgagcagga tcgcgtgtcg gtgatctcgt tcgacgaggc cagggaagtc
      781 gccagcgtgc cggtgggcga tcacccgcag cgcgtgcgga cggggaagct gctgctgccg
      841 taggtcggca atcatttgcc gacgactact gcggcatggg aatgccgccc tacgcctctc
      901 cccacgcagt ggggagaggg aattagagat caggacttct gccgcagcac cagcagcatc
      961 ccgccaacga ccagcgcgcc gatgccggcc cacaccggga tgttgaggta gtccttttcc
     1021 ttgaccgtca tgtgcagcgg gccgaggtcg gcccgatgcg tgtccgacgt gtagctgaag
     1081 ccgccgtaca cgagggccag cgcgccggcg atgagcagca ggatgcccgc gatcttcgcg
     1141 ccgctcatac gactctccgg ccctggatga cccggaccag cacgacgacg acggcgacga
     1201 ccagcagcag gtggatgagt ccgcccatgg tgtacgagga cacgaaccct acggcccaga
     1261 ggaccagaag gaggatggcg atggtttcga gcatgggttt ctccggacag taagaccgcg
     1321 aacggcagga caccagcttg ggcgcacgcg gtgcgccaat ctgtgcggca ggacaccgtc
     1381 gcagcccatc agctgcagca ggcgcgcccg gacgctttca taccgcctcc tgaatggggc
     1441 cggtgccagg gcctgatcac gcgattcggg atcgcgggag gcgcatgcag acggcccggg
     1501 ccgggagccg atcgtggctt cccggaagcc cactatcgac cggcccggcg cgcccgtatg
     1561 tacggacgcg cacccacagg cttgtggtcc tgtttcaacc cttgtgtgtg gcaccgtacg
     1621 gaaatgcgcc ggcccactgc gcatactgca acggacaggc ctgtgtcccg aatttgcagg
     1681 agcgtgaagt gccggtcgcc aaagtgaacg tcgtcaacca gctgatccgg gatctgccgc
     1741 aaaagctgcg cgcccggctg ctggcccgct gcgagcaagt cgagctcgtg ttcggcacga
     1801 tcctgtgcga ggccaacgtg ccgtttacgc acgtgtactt cccgctcaga gggttcattt
     1861 cgctggtggc gaccgtggac gaccacgagc cgctggagat ggggctgatc ggcaacgagg
     1921 gcatgctcgg cgccaccatc accctgggcg tcgatgtcgt gccgctgcgc ggcgtcgtgc
     1981 aagggtccgg cagcgcgctg cgcatgagcg ccgcccgctt cagggccgag ttgcgcgaca
     2041 gcccggatct gcgccgcatg gtcaaccgct acctgtacgt gctgatggcg ca
//
LOCUS       DASWIY010000134         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3044903, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000134 DASWIY010000000
VERSION     DASWIY010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3044903"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGA59_05155"
     CDS             <1..267
                     /locus_tag="VGA59_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEX9719100.1"
                     /translation="FTTGRGSAYGCAPSPSLKLATNSALWQRQEEDMDINCGEIIDGK
                     ASIQEMGERIFALVLATASGELSKSEKHGYGQNEFVPWQVGAVM"
     gene            318..1391
                     /locus_tag="VGA59_05160"
     CDS             318..1391
                     /locus_tag="VGA59_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HEX9719101.1"
                     /translation="MAVTFSAEALRTQVAGILAAAGSSAEEARTVADNLVLANLSGHD
                     SHGVGMVPRYVDAVLEGGLTPNAGVKVTLDGGALLALDGQRGYGQVVGEQAMALGMAR
                     ARQAGSCIMALANAHHLGRIGHFAEMATQQGLVAIHFVNVMSRPTVAPWGGADGRFGT
                     NPCCIGVPLKGREPFLLDFATSRVAQGKMRVAHNEGRRVEPGYLLDPRGLPTTDPGVV
                     VVPQTRDAGAGLFGALLPFGEHKGYGLAVACELLGGALTGSGTWHRPADTARAVVNGM
                     LTILIDPHKLGTDASFEREALAFIDWLRQSPPAPGSDGVRLAGEPEREARALREREGI
                     AIDDQTWAEIVAAGAKVGFTGLS"
     gene            complement(1434..2138)
                     /gene="pdeM"
                     /locus_tag="VGA59_05165"
     CDS             complement(1434..2138)
                     /gene="pdeM"
                     /locus_tag="VGA59_05165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEX9719102.1"
                     /translation="MLRIRVADADVVLHPSGAALLSADQTLLVADAHFGKAVSFRKLG
                     VPVPAGTTTETLDELSAAVAETKARRIVFLGDFLHSRHSHAAGTLDALGRWRAAHAGL
                     ELTLVRGNHDDRAGDPPASLGIRVVDEPLARGSFALCHHPRAVAGAYVLAGHWHPCIS
                     VSGRAFERLRLPCFWFGDDTGELPQHAVGVLPAFGSFTGMHRIEPRAGDRIFPVAGDV
                     VRAIPALPLDPGSSPG"
BASE COUNT          381 a          768 c          807 g          336 t
ORIGIN      
        1 ttcacgaccg gccgcggctc ggcctacggc tgcgccccct cgccttcgct caagctggcg
       61 accaacagcg cgctgtggca gcggcaggaa gaggacatgg acatcaactg cggcgagatc
      121 atcgacggca aggcctcgat ccaggagatg ggcgagcgca tcttcgccct ggtgctggcg
      181 accgcctcgg gcgagttgtc caagagcgag aagcacggct atgggcagaa cgaattcgtg
      241 ccctggcagg tcggcgcggt gatgtgattt caactccctc tcccctccgg ggagagggct
      301 ggggtgaggg gcacacaatg gcagtaacct tttccgccga ggccttgcgc acgcaagtcg
      361 ccggcattct ggccgcggcc ggcagcagcg ccgaagaggc gcgcacggtg gcggacaacc
      421 tggtgctggc caacctcagc ggtcacgatt ctcatggcgt gggcatggtc ccgcgctacg
      481 tcgatgccgt gctcgaaggt gggctgacgc ccaatgccgg cgtgaaggtg accctggacg
      541 gcggcgccct gctggcgctg gacgggcagc gcggctacgg ccaggtggtc ggcgagcagg
      601 ccatggccct gggcatggcg cgcgccaggc aagcaggcag ctgcatcatg gcgctcgcaa
      661 atgcccacca cctgggccgc atcgggcatt tcgcggagat ggccacgcag caggggctgg
      721 tggccatcca tttcgtcaat gtgatgtcac gcccgacggt tgcgccgtgg ggaggcgccg
      781 acggccgctt cggcaccaat ccgtgctgca tcggggtgcc gctgaagggc cgcgaaccgt
      841 tcctgctgga cttcgccacc agccgcgtcg cgcagggcaa gatgcgcgtg gcccataacg
      901 aaggccgccg ggtcgaaccc ggttacctgc tcgatccccg cggcctgcct accaccgacc
      961 ccggggtcgt tgtggtgccg cagacccgcg atgcgggcgc cggcctgttc ggcgccctgc
     1021 tgcctttcgg cgaacacaag ggttacggcc tggcagtggc ctgcgagttg ctgggcggcg
     1081 cgctcaccgg cagcggaacc tggcaccggc cggccgacac ggcgcgagcg gtggtcaacg
     1141 gcatgctcac catactgatc gatccgcaca agcttgggac cgatgcaagc ttcgagcgcg
     1201 aagcgctggc tttcatcgac tggctccggc agagcccgcc ggcgccgggt tcggacggcg
     1261 ttcgcctggc cggcgagccc gaacgcgagg cccgggcgct gcgcgaacgc gaaggcatcg
     1321 cgatcgacga ccagacctgg gccgaaatcg tcgcggcggg cgcgaaggtg ggcttcacgg
     1381 ggctgtcgtg agaagtcatg gattgcgggt cgagcccgca atgacaatac gtgtcatccc
     1441 gggcttgacc cgggatccag cggcagcgcc ggaatcgcgc gtaccacgtc ccccgccacg
     1501 ggaaagatcc ggtcgccggc acgcggctcg atacggtgca tgcccgtgaa gctgccgaac
     1561 gccggcagca caccgactgc gtgttgcggc aactcgcccg tgtcgtcgcc gaaccagaag
     1621 cacggcaagc gcagccgctc gaaagcgcgg ccgctcacgc tgatgcacgg gtgccaatgt
     1681 ccggccagca cataagcccc ggccacggcc cgcggatgat ggcatagcgc gaacgatccc
     1741 cgtgccaggg gctcgtcgac cacccggatg cccaggctgg ccggcggatc gccggcgcgg
     1801 tcgtcgtgat tgccgcgcac cagcgtcagt tcgaggccgg catgcgcggc cctccagcgg
     1861 cccagcgcgt cgagcgtgcc cgcggcgtgg gaatgtcgcg agtgcaggaa gtccccgaga
     1921 aaaacgatgc gccgcgcctt ggtctcggcg acggcagccg aaagctcgtc cagtgtctcg
     1981 gtcgtggtgc cggcgggcac cggcacgccg agcttgcgaa agctcaccgc cttgccgaaa
     2041 tgggcatcgg ccacgagcag ggtctggtcg gccgacaaca gggccgcccc gctgggatgc
     2101 aggacgacgt cggcatcggc gacacggata cggagcatgt cagtccttca tcctggcgct
     2161 agcctgtatc tgtcatcctg ggcttgcatt tgtcatcccg ggcttgaccc gggatccatg
     2221 gattgcgggt caagcccgca atgacaagac ctgcgggtca agcgcaatga caagacctgc
     2281 gggtcaagcc cg
//
LOCUS       DASWIY010000135         6839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2670699, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000135 DASWIY010000000
VERSION     DASWIY010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2670699"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1515)
                     /locus_tag="VGA59_05170"
     CDS             complement(232..1515)
                     /locus_tag="VGA59_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX9719103.1"
                     /translation="MESYLLDWSNLLLRWAHVIVAIAWIGSSFYFVFLDSNLTPPVDP
                     DLKKQGVDGELWAVHGGGFYHPLKFNVAPPQLPGDLHWFYWESYSTWLTGFALFAISY
                     LWSPSTYLVDKSVMDWPPGAAIAVALSFLAVFWILYDAICRVFGQRTFRWAAPRKVGP
                     LGGQRTTRSAERGGHIPGDAIVGALVLVLVCVASWLACQWFAGRAAFLLVGAMIATAM
                     SANVLVWIIPGQRKVVAAIEAGQAVDPIHGSRGKQRSVHNTYFTLPVLFSMLSNHYSF
                     TWSHPRNWLVLILMMFAGAAIRQFFVMRHGWKLGRNRHPLPYAAVGVAVIAGVVAWLR
                     PDAPSNIASAAPRAVGYAEVQQVLAQRCHLCHGAQVQMKNVRLDTPAALKQHAQAVYQ
                     QAVVSRLMPMNNATGITEAERALIAQWFQGGAPAP"
     gene            complement(1539..2345)
                     /gene="xdhC"
                     /locus_tag="VGA59_05175"
     CDS             complement(1539..2345)
                     /gene="xdhC"
                     /locus_tag="VGA59_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEX9719104.1"
                     /translation="MNVVTSRLMSEDAVLVGVEATQGSVPREAGTWMAVFAGSVVGTI
                     GGGQLELQAIEEARRRLAGGEGEPLRRYALGPSLGQCCGGVMHLRFERIGAADIAALQ
                     ARLAPRLAPLALFGGGHVGKALVTVFSTLPFAVTWIDSRDEIFPAQVPDHVLCEHSDP
                     VHAAVADLAALSRVLIMSFSHAEDLDIVAACLRRRRERGDLPYIGLIGSKTKWATFRH
                     RLEDRGFTAAELAQVTCPIGLPGITGKEPEVIAVSVAAQILQAAPPVAKP"
     gene            2483..2989
                     /locus_tag="VGA59_05180"
     CDS             2483..2989
                     /locus_tag="VGA59_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719105.1"
                     /translation="MKHISGLRFTVPSAWAAIVAALLAACSSRPPSPPAPVDVVTPAP
                     VQSRPSAAGARSSGATNARAYRADAASHLYGLNQERIYKGRLPPMLYAIGVLQVEVDG
                     RGNVRNLNWMRAPRHAPEVVAEIERTVRAAAPFPAPARMGKVVYTDTWLWHKSGRFQL
                     DTLTEGQD"
     gene            complement(3160..3513)
                     /gene="uraH"
                     /locus_tag="VGA59_05185"
     CDS             complement(3160..3513)
                     /gene="uraH"
                     /locus_tag="VGA59_05185"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEX9719106.1"
                     /translation="MGLSTHVLDTMHGCPAAGMAVELYTTKGDSTDLVKRFTLDADGR
                     SPDGPLYASGSLRKGTYRLVFDVAAYFRGRGVALPEPPFLGKVGLDFGVAHPDQHYHV
                     PLLVSPWSYSTYRGS"
     gene            3614..4567
                     /gene="puuE"
                     /locus_tag="VGA59_05190"
     CDS             3614..4567
                     /gene="puuE"
                     /locus_tag="VGA59_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HEX9719107.1"
                     /translation="MIYDSTAPYPRDLAGYGRNPPHAQWPGGARIAVQFVLNYEEGGE
                     NSVLHGDAGSEQFLSEMFSPPSFQARHLTMESIYEYGSRAGAWRILREFERRNLPLTV
                     FGVSMALQRHPDLTAAFRQLGHEIACHGWRWIQYQNLDEATEREHMKTGMRIIEELTG
                     ERPLGWYTGRDSPNTRRLVADYGGFEYDSDYYGDDLPFWMKVRKTDGAVVPQLIVPYT
                     LDTNDMRFALPQGFAQAQDFFIYLRDSFDALYAEGDPGGEDRPRMMSVGMHCRLLGRP
                     GRIVALQRFLDHVQKHDRVWICRRIDIARHWKQTHPYKAAP"
     gene            4564..6375
                     /gene="uraD"
                     /locus_tag="VGA59_05195"
     CDS             4564..6375
                     /gene="uraD"
                     /locus_tag="VGA59_05195"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEX9719108.1"
                     /translation="MTLTLDQLNAAPLEEARQLLQGLYEHSPWIAEGALARRPFRSLA
                     HLKHTMARLVSDAGRQAQLTLIRAHPELAGKAMVANTLTAESALEQSKAGLAHCTPEE
                     FARLQKLNADYNARFGFPFILAVRGPRGTGLSRQEVLDTFQRRLDNPPDHELAECLRN
                     IHRIVELRLNDKFGVEPALGNLVWDWQEQLAQFSDSGFKEKGQLTVTYLTDAHRAVAA
                     RIAANMRDSGFDEVAIDAVGNVAGRYWASPHPSPLPKGEGARAKTLLTGSHYDTVRNG
                     GKYDGRLGIFTPMACVRELSREGRRLPFDIEVIGFAEEEGQRYNATFLGSGALIGAFN
                     PAWLDQRDADGVTLREAMQRAGLPATMEAIAALRRRPPDYLGFVEVHIEQGPVLAGND
                     LPLGIVTSINGGVRYQCEIIGMASHAGTTPMDRRRDAATAAAELALYVEQRAAQDGDS
                     VGTIGILTVPGGSTNVVPGRCQFTLDLRAPTDAQRDALVKDVLTQLRAVCERRGLRHT
                     VEETMRAAAAPCAPAWQRRWERAVDALGVPLHRMPSGAGHDAMKLHEVMPQAMLFVRG
                     QNSGISHNPLESTTSDDIQLAVEAFQRLLGDLEGQTA"
     gene            6372..>6839
                     /locus_tag="VGA59_05200"
     CDS             6372..>6839
                     /locus_tag="VGA59_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9719109.1"
                     /translation="MTDCEKLDAWVDAHFDEEVRFLQELVRVPTDTPPGDNAPHAEHT
                     AELLAALGFEPEKHPVPLSDVRARGLESITNLIVRRRYGPGPTIALNAHGDVVPPGEG
                     WTHDPYGAEIEDGKLYGRGAAVSKSVFASFTFAVRALESLRSQLNGGVELHFTY"
BASE COUNT         1224 a         2346 c         2260 g         1009 t
ORIGIN      
        1 ctggggtcgg gcgcgcttgc gcgcttcgtc aactgactcg tcgcagttgt ctgagcgaag
       61 ggcgccccaa gcgcccgtag cgagttctgc gacgccgacc ccaggccgag cagcgcagcg
      121 gagtcggagc gaagcggaga ccgcaacagt gtgagccccc gccgggtacc gcctgccgcg
      181 acccccataa cgttgcccgg tcccgcgcat cggggcttca atgcaggctc cctaaggagc
      241 cggcgccccg ccctggaacc actgcgcgat cagggcacgt tcggcttcgg tgatgccggt
      301 ggcattgttc atcggcatga gccgtgacac caccgcctgc tggtacacgg cctgggcatg
      361 ctgcttgagg gcagcgggcg tatcgagccg cacgttcttc atctggacct gcgcaccgtg
      421 gcacaggtgg cagcgctgcg ccagcacctg ctgcacttcc gcgtaaccga cggcgcgcgg
      481 cgcagcggag gcgatgttcg agggcgcatc cgggcgcagc catgcaacca cgcccgcaat
      541 cacggcaacg cccaccgccg catagggcaa gggatgccgg ttgcggccca gcttccagcc
      601 gtggcgcatc acgaagaact gccggatcgc ggcgccggcg aacatcatca ggatcagcac
      661 cagccagttg cgcggatggc tccaggtgaa gctgtagtgg ttgctcagca tcgagaacag
      721 caccggcagc gtgaaatagg tgttgtgcac gctgcgctgc ttgccgcgac tgccgtggat
      781 ggggtccacc gcctgtcccg cctcgatggc cgccaccacc ttcctctggc ccgggatgat
      841 ccagacgagg acattggcac tcatggcggt ggcgatcatc gcgcccacca gcaggaaggc
      901 agcccgcccc gcgaaccact ggcacgccag ccaggaagcc acgcagacca gcaccagcac
      961 caaagcgccg acgatggcat cgccgggtat gtggccccca cgctccgcac tgcgtgtcgt
     1021 ccgctgcccc ccgagggggc ccactttcct cggggcggcc cagcggaaag tgcgctggcc
     1081 gaacaccctg cagatcgcgt cgtagaggat ccagaacacc gccaggaacg acagcgccac
     1141 ggcgatggcg gcaccgggcg gccaatccat gaccgacttg tccacgaggt aggtgctcgg
     1201 gctccaaagg taggagatcg cgaacagcgc gaagccggtc agccaggtcg aatagctttc
     1261 ccagtaaaac cagtgcaggt caccgggcag ctggggcggc gcgacgttga acttgagagg
     1321 gtgatagaag ccgccgccgt gcaccgccca caactccccg tcgacgccct gcttcttgag
     1381 gtccggatcc accggcggag tcagattgga atcgaggaag acgaaataga aggacgagcc
     1441 gatccaggcg atcgcgacga tgacgtgcgc ccatcgcagc agcaggttgg accagtcgag
     1501 gaggtagctt tccatgggcc ttcagtttcg cggcggcatc acggttttgc gaccggcggc
     1561 gccgcctgca ggatctgcgc tgcaacgctc acggcgataa cctccggctc cttgcccgtg
     1621 atgccgggca ggccgatcgg gcaggtcacc tgagcgagct ccgccgcggt gaacccacgg
     1681 tcctcaagcc ggtgccggaa cgtggcccac ttggtcttgc tgccgatgag gccgatgtaa
     1741 ggaagatcgc cgcgttcgcg ccggcgccgc aggcacgccg ccacgatatc caggtcctcc
     1801 gcgtggctga aactcatgat cagcacgcgc gagagggccg ccaggtccgc caccgccgcg
     1861 tgcaccgggt ccgagtgctc gcaaaggaca tggtccggta cctgcgccgg aaagatctcg
     1921 tcgcggctgt cgatccaggt cacggcgaac ggcagggtcg agaacacagt caccagcgcc
     1981 ttgcccacgt ggccgccgcc gaacagcgcg agcggcgcca gccgcggggc aagccgcgcc
     2041 tgcagtgccg caatgtcggc ggcaccgatg cgctcgaacc gcaggtgcat cacaccgccg
     2101 cagcactgac ccaggctggg gccgagggca tagcggcgca gcggctcgcc ctcgccgccc
     2161 gccagccggc ggcgcgcctc ctcgattgcc tgcagttcga gctggccgcc accgatcgtg
     2221 cccaccacgc tgccggcaaa aacggccatc caggtgccgg cctcgcgcgg gaccgagccc
     2281 tgcgtcgcct cgacgccgac cagcaccgcg tcctcactca tcaaccggga tgtgacaaca
     2341 ttcatggaaa tttcgcgttg acaagcgtcc agtcgcaaca ttcgtagccg tgaggtaaac
     2401 ccggtcgtca aaccagacag ttccgcgttg aatagctcag ctgcgacctc gccgtcaagc
     2461 gtacaaatcc aggagacaaa cgatgaagca tatttccggc ctgcgcttca cggtgccgtc
     2521 ggcctgggcg gccattgtcg cggcgctgct ggcggcttgc tcctccaggc cgccgtcgcc
     2581 acccgctccg gtggatgtcg tcacgccagc tcccgtgcag tcccggcctt ccgcggccgg
     2641 cgcgcgctcc tcgggtgcaa ccaacgcgcg tgcgtaccgc gcggacgccg ccagccacct
     2701 gtatggcctg aaccaggagc gtatctacaa gggcaggctg ccgccgatgc tgtacgccat
     2761 cggcgtgctc caggtcgaag tcgatggccg gggcaacgtg cgcaacctga actggatgcg
     2821 cgcgcccagg cacgcaccgg aggtcgtggc cgagatcgag cgcacggtgc gcgccgccgc
     2881 gcccttccct gcgcccgccc gcatgggcaa ggtggtctat acggacacct ggctgtggca
     2941 caagagcggc cggttccagc tcgacacact gaccgaggga caggattgaa ggcctggatt
     3001 gcggatcaag tccgcaatga caaaccagaa aaactgtcat cccgggcaga gcctgccccg
     3061 gacttgatcc ggggacccgg gatccaggct tgctcgcgga catggattgc ggatcaggcc
     3121 tgccccggac tccgatccgg ggtccgcaat gacagcattt catgagccac gataagtcga
     3181 gtagctccac gggctcacca gcaggggcac atggtagtgc tggtccggat gggccacgcc
     3241 gaagtcaagg ccgaccttgc ccaggaaagg gggctccggc agcgccacgc cccggcccct
     3301 gaaataggcg gcaacgtcga acaccagccg gtaggttcct ttcctcaggc tgccgctggc
     3361 gtacagcggc ccgtcggggc tgcgcccgtc ggcatccagt gtgaaccgct tcaccagatc
     3421 cgtgctgtcg cccttggtcg tgtacagctc caccgccatg cccgcggccg ggcacccatg
     3481 cattgtgtcc aggacgtgcg tgctcaggcc catgggagaa tcctcgaagt tcgtccacag
     3541 gagtgtatac acaggccttg gcggcaaggc ccagcttcga tacatcgccg tcggccaatg
     3601 tcaacccagc accatgatct acgacagcac agccccctac ccgcgcgacc tggccggcta
     3661 cggacgcaac ccgccccatg cgcaatggcc gggcggcgcg cgcatcgcgg tgcagttcgt
     3721 cctcaactat gaagagggcg gcgagaactc ggtgctgcac ggcgacgcgg gctcggagca
     3781 attcctgtcc gagatgttca gcccgccgag cttccaggcg cgccacctga ccatggagag
     3841 catctacgaa tacggctcgc gcgcgggtgc gtggcggatc ctgcgcgagt tcgaacggag
     3901 aaacctgccg ctgacggtgt tcggcgtgag catggcgctg cagcgtcacc cggacctcac
     3961 cgcggcgttc aggcaactgg gacacgaaat cgcctgccac ggctggcgct ggatccaata
     4021 ccagaacctc gatgaagcga ccgagcgcga gcacatgaag accggcatgc ggatcatcga
     4081 ggagctgacg ggagagcggc cgctgggctg gtacaccggc cgcgacagcc cgaacacgcg
     4141 gcggctggta gccgactacg gcggtttcga atacgacagc gactattacg gcgacgatct
     4201 tccgttctgg atgaaggtgc gcaagaccga tggcgccgtg gtgccgcagc tgatcgttcc
     4261 ctacacgctg gacaccaacg acatgcgctt cgcgctgccc cagggattcg cacaagcgca
     4321 ggatttcttc atttacctgc gcgacagctt cgacgcgctt tacgccgagg gcgaccccgg
     4381 cggcgaggac cggccccgga tgatgagcgt ggggatgcac tgccgcctgc tgggccggcc
     4441 ggggcgcatc gtcgcgttgc agcgatttct cgatcatgtg caaaagcacg atcgcgtctg
     4501 gatctgccgc cgcatcgaca tcgcgcgcca ctggaagcag acccatcctt acaaggcggc
     4561 cccgtgaccc tgaccctgga tcaactgaat gcggcgcccc tcgaggaagc caggcagctg
     4621 ctccagggcc tgtacgagca ttcgccctgg atcgccgagg gggcgctggc gcggcgcccg
     4681 ttccgctcgc tcgcgcacct caagcacacc atggcgcgcc tcgtgtccga tgccggccgg
     4741 caggcccagc tcacgctgat ccgggcccac cccgagctcg ccggcaaggc catggtcgcc
     4801 aacacgctga cggcggagtc ggcccttgag cagagcaagg cgggccttgc gcattgcacg
     4861 ccagaagagt tcgccaggct acagaaactg aacgccgact acaacgccag gttcggcttt
     4921 ccattcatcc tcgcggtgcg cggtccgcgc ggcaccgggc tgagccggca ggaggtcctc
     4981 gataccttcc agcgccgcct cgacaatccg ccggaccacg agctcgcgga atgcctgcgc
     5041 aacatccatc gcatcgtgga gctgcgcctc aacgacaagt tcggcgtcga accggccttg
     5101 ggcaatctgg tgtgggactg gcaggaacag ctggcccaat tcagcgactc gggtttcaag
     5161 gagaagggcc agctcacggt cacgtatctc acggacgcgc atcgagcggt ggcggcgcga
     5221 attgccgcga acatgcggga cagcggcttc gatgaggtcg cgatcgacgc ggtcgggaat
     5281 gtcgcggggc ggtattgggc cagccctcac cccagccctc tcccaaaggg agagggagca
     5341 agagccaaga cactcctcac cggcagccac tacgacacgg tgcgaaacgg cggcaagtac
     5401 gacgggcggc tggggatttt cacgcccatg gcctgcgtgc gtgaactgtc gcgcgagggc
     5461 aggcgcctgc ccttcgacat cgaggtgatc ggcttcgccg aggaggaggg ccagcgctac
     5521 aacgccacct tcctcggctc gggcgcgctg atcggcgcct tcaatcccgc atggctggac
     5581 cagagggatg ccgatggcgt caccctgcgg gaagcgatgc agcgcgccgg gctgccggcg
     5641 acgatggagg cgatcgccgc gctgcggcgc cggccgccgg attacctcgg cttcgtcgaa
     5701 gtccacatcg agcagggccc ggtccttgcc ggcaacgatc tgccgctggg catcgtgacg
     5761 tcgatcaacg gcggcgtgcg ctaccaatgc gagatcatcg gcatggccag ccatgcgggc
     5821 accacgccga tggatcgccg ccgcgatgcg gccaccgccg ccgccgagct cgcactctac
     5881 gtggaacagc gagccgcgca ggacggcgat tcggtcggca ccatcggcat tctcaccgtg
     5941 ccgggcggat ccaccaatgt ggtgccgggg cgctgccagt tcacgctgga cctgcgtgcc
     6001 ccgaccgatg cgcagcgcga cgccctggtc aaggacgtcc tgacgcagct gcgggcggtg
     6061 tgcgaacggc gcggcctgcg ccacacggtg gaggagacca tgcgcgccgc ggccgcgcct
     6121 tgcgcgccgg cgtggcagcg gcgctgggaa cgcgcggtcg acgcgctggg cgtgccgctg
     6181 caccgcatgc ccagcggcgc cggccatgac gcgatgaagt tgcacgaggt gatgccgcaa
     6241 gccatgctgt tcgtgcgcgg ccagaattcc ggcatcagcc acaacccgct ggagagcacg
     6301 acgagcgacg atatccagtt ggcggtggag gctttccagc ggctcctggg cgacctcgag
     6361 ggacagacgg catgaccgat tgcgagaagc tggacgcctg ggtcgatgcg cacttcgacg
     6421 aggaagtgcg ttttctgcag gagctggtgc gcgtaccgac cgacacgccg ccgggcgaca
     6481 acgcgccgca cgcggaacac acggccgagc tgcttgccgc acttggcttc gagcccgaga
     6541 agcatccggt tcccctgtcc gatgtacgcg cccgtgggct cgaatcgatc accaacctga
     6601 tcgtgcgccg ccgctatggg ccgggcccga cgattgcgct gaacgcccac ggcgacgtgg
     6661 tgccgccggg cgaaggctgg acgcacgatc cgtacggcgc cgagatcgag gacggaaaac
     6721 tctacggccg cggggccgct gtgagcaaga gcgttttcgc cagcttcacc ttcgccgtgc
     6781 gcgcgctcga atcgctgcgc tcgcagctca acggcggggt ggaactgcac ttcacttac
//
LOCUS       DASWIY010000136         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_75347, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000136 DASWIY010000000
VERSION     DASWIY010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75347"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VGA59_05205"
     CDS             complement(<1..874)
                     /locus_tag="VGA59_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9719110.1"
                     /translation="MFAAPQELRAEVFARLPDALRVRNRSSRWADVQRGGAPTDSFLE
                     GPSFDVSGRLYLVDIPWGRIFRVSPEGEFELFTEYDGEPNGLKIHRDGRIFVADFRRG
                     LMVVDPQTRRVEPHLDESVQAGFKGLNDLHFALNGDLYFTDQGLTGLQDPSGRLYRLR
                     SNGTLDLLLDNVPSPNGLVLNLAENVLYLNVTRGNCIWRVPMAADGTPFKVGVFVQLS
                     GGLGGPDGLAIDEEGNLAVAHFGLGTVWLFSALGEPLYRIRSPLGLATTNVAYRDRFL
                     YITESETGTVLRVEL"
     gene            complement(897..1433)
                     /locus_tag="VGA59_05210"
     CDS             complement(897..1433)
                     /locus_tag="VGA59_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9719111.1"
                     /translation="MSDLSQYSVSARAILDVFRAKAISHVTTVPDYAQLSLQMRLEEG
                     YLPDTRVMRCCTEDQAVECSAGLWIGGKKAVVIMQNQGFYACVNSVRAVGIDAKLPLV
                     LMIGPWGREFANLGQDPRNSRRRVVAMLEPMLDTLGIRHWRLESASDLGNVGAAIDYA
                     YGESAPAAVVVGAYTAWQ"
     gene            complement(1451..>2176)
                     /locus_tag="VGA59_05215"
     CDS             complement(1451..>2176)
                     /locus_tag="VGA59_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9719112.1"
                     /translation="APDGYTIMMGTLGTHSIAPTLYPKLGYNVARDFKPIANFAYLTN
                     YVVAGPSLKAADVADLIRTAKAQPGKITFASAGNGTPAHLAIELFRIRTGADVLHVPY
                     KGGAPAITDLLGGHVDAIFGDPVSVLPHIASGKLRALAFAGPRRSAALPSVPTTAEAG
                     LADFQVRAWHGVLAPAGVSPEIARKLADAVHAATRESAVQAAMARMGAEPGDEILEEF
                     AQVIQAESVKWGEVVKRANVKLD"
BASE COUNT          388 a          731 c          681 g          376 t
ORIGIN      
        1 gaagttcgac cctgagaacg gtccccgttt cactttcggt gatgtagaga aagcggtcgc
       61 gataggcgac attggtcgtg gccagtccga gcggcgagcg aattcgatag agaggctcgc
      121 ccagcgcgct gaacaaccac acggttccga ggccgaagtg cgcaacggcc aggttgcctt
      181 cttcgtcgat ggccaggcca tccgggccac cgagtccgcc cgacagttgc acgaaaacgc
      241 ccaccttgaa cggcgtcccg tccgcagcca tcggcacccg ccagatgcaa ttgccccggg
      301 ttacgttcag gtacagcacg ttctcggcga gattcagcac cagcccgttg ggactgggaa
      361 cattgtccag cagcaggtcc agcgtcccgt tagaccgcag ccggtacagc cgccccgagg
      421 gatcctgaag accggtgagc ccctggtccg tgaaatacag atcgccgttc agcgcaaagt
      481 gcaggtcgtt cagccctttg aagcctgcct ggaccgattc gtccagatgg ggctccacgc
      541 gcctggtttg cggatccacg accatcaggc cgcgccggaa gtccgccacg aagatgcggc
      601 cgtctcggtg aatcttcagt ccgttgggct caccgtcgta ctcggtaaac agctcgaact
      661 cgccttcggg cgacactctg aagatgcgtc cccatgggat gtccacgagg tagaggcgcc
      721 ccgacacatc gaaagacggc ccctcgagaa atgagtcggt cggcgcgccg ccacgctgca
      781 catcggccca ccggctggaa cggtttcgca cgcgcaacgc gtcgggcagg cgcgcgaaga
      841 cctcggcacg aagctcttgg ggcgcagcaa acatgagcgg caacgcccgc gtgaagctac
      901 tgccaggccg tgtaggcgcc caccacaacc gccgcgggtg cgctttcgcc gtaggcgtaa
      961 tcgatggcag cgccgacatt gcccagatcc gacgcggact ccaggcgcca atggcgaatt
     1021 cccaatgtgt cgagcatcgg ctccagcatg gccaccaccc gccggcgcga attgcgcggg
     1081 tcctggccaa ggttggcgaa ttcgcgtccc caggggccga tcatcagcac cagcggtagc
     1141 ttggcatcga ttcccactgc acggacggaa ttgacgcatg catagaagcc ctggttctgc
     1201 atgatcacca cggccttctt gccgcctatc cacaggcccg ccgagcattc gacggcctgg
     1261 tcctccgtac agcagcgcat cacccgcgtg tcgggcagat agccctcctc caggcgcatc
     1321 tgcagcgaaa gctgggcgta gtccggcacc gtcgtgacgt ggctgatggc cttggcgcga
     1381 aaaacatcga ggatggcgcg tgcgctgacg gagtactggc tgagatcgga catgctgctt
     1441 ttcctttgct tcaatcgagc ttgacgttgg cccgcttgac cacctcgccc cacttcacgc
     1501 tctcggcctg gatcacctgt gcgaactcct cgagaatttc gtcgccgggc tcggcgccca
     1561 tcctggccat cgcagcctgg acggcgctct cgcgcgtcgc cgcatgcacc gcatcggcca
     1621 gcttcctggc gatctcgggg ctcacgcccg ccggcgccag cacgccatgc catgcccgca
     1681 cctgaaagtc ggccaggccg gcctcggccg ttgttggaac gctgggcagg gccgcgcttc
     1741 tcctgggccc cgcaaacgcg agtgcgcgca acttgcccga tgcgatgtgc ggcagcacgc
     1801 tgaccgggtc tccgaagatg gcatcgacgt gaccgcccag caggtcggtg attgccggcg
     1861 ctccgccctt gtacggaacg tgcaggacgt cggcgccggt gcggatgcgg aacaactcga
     1921 tcgccaggtg cgccggcgtg ccgttgccgg ccgacgcaaa ggtgatcttg cccggttggg
     1981 ccttcgccgt cctgatcaga tccgcaacat cggccgcttt caacgacggc ccggcgacca
     2041 catagttggt caggtaggca aagttcgcga tcggcttgaa gtcgcgagcg acgttgtacc
     2101 cgagcttcgg gtagagggtt ggcgcgatgg agtgggtgcc cagcgtcccc atcatgatcg
     2161 tgtatccgtc cggtgc
//
LOCUS       DASWIY010000137         6259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1940908, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000137 DASWIY010000000
VERSION     DASWIY010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940908"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VGA59_05220"
     CDS             complement(<1..873)
                     /locus_tag="VGA59_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9719113.1"
                     /translation="MMFKQSNRSDLPAPRRRVARLSLACALAMTPLAGWPQAPAGPAP
                     VPAAAPAAAPLTLSQAYRAAMEQDAVIRTARAAADARRERLPQARAQMLPNISASLAR
                     NKNDLTSTQPGLGAVPVTLDQKYMSGTRALTLRQPIYRPFLIADYRQAEAQVAEANAV
                     LDKEVQNVAVRVSGAYLEALLAQDQLALVMVQKQTYTTHLDAARKRFAAGAGVRTDID
                     EAQAKLDLTLALELEARQNVDFTRRQLQVMVNMPVDQLAPLDERKLELARPIPDRLED
                     WTERAELSSPDLRSL"
     gene            complement(870..2243)
                     /locus_tag="VGA59_05225"
     CDS             complement(870..2243)
                     /locus_tag="VGA59_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEX9719114.1"
                     /translation="MAKPELQLLKTRNDSAPSMEVALAGVKPALEGSSRRASRVGLWA
                     LVLGLGGFMVWAALAPLDEGVPSQGLVAINTKRKAVQHPTGGIVKEVLVGEGDRVKEG
                     QLLLKLDEAAARANYEASRQRYLSLRTMQGRLLAEQAGLSTIVFHPDLQSASQDPLIR
                     SQMQTQEQLFRARRTALRADLQALQEGIQGQEALLQSYQGILPNRRNQISLLDEELTH
                     TRSLVKDGYAPRNRQLELERMVAEANSSVAELLGNTTRSQRAIGELRQRLISKQQEYR
                     KEVETQLTEVTREVQSEEGKFHALSDDLSRIEIKSPAAGQVVGLAVQTVGGVIQSGQK
                     LMDIVPENELLLIETRVAPNLIDRVQAGLPVDIRFNSFANSPSLVVDGQVASISGDLL
                     TDPQSGVQYYLSRVSVTPEGYKKLGKRVLQPGMPVEVVFRTGERSLLAYLMHPLSKRL
                     AAAMKEE"
     gene            complement(2288..4027)
                     /locus_tag="VGA59_05230"
     CDS             complement(2288..4027)
                     /locus_tag="VGA59_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEX9719115.1"
                     /translation="MNKPGFFQRSELTAALWAFRREFLLVGLFSMVANLMMLAPTLYL
                     LQVFDRVMVSQSELTLVAVSLITLFLFGVMAVSEWMRSKILVGAGIRFDEQLSTRVFN
                     ASFESSLSQSSASPNRAFADLITLRQFITGAGIFALFDTPWTPIYIAVTFFLHPWLGF
                     VSLVFVVVQGALAWFGHRSTVAPSEAASKAGADVNGYLQSKLRNAEVIESMGMLGNLR
                     SRWRRSQDEYLAKSTQAQNVSTRVTAWSKYIRYSQQSLSLAAGAVLVIDGQLTPGGMI
                     AVNLLMARALAPIDQLVTVWRSFLSTRTAFQRLERLLVEYPERDPNLTRVAPQGEVIL
                     REVVADAPGRATPILKGISFSVAAGTITAIVGPSGSGKSTLARVMMGIWPDVSGEVLL
                     DGLPIQGWDRTELGPHLGYLPQDIELFEGTIGENIARFGELDSDKVIEAARCAGLHEM
                     ILRFPKGYDMPIGEGGHLLSGGQRQRIGLARAVYGDPAVLVLDEPNSNLDDAGEAALL
                     KTVLQLKSKGKTVFLITHRGGAVAVADRIMVLQDGRIKADGPREATLAGLLPQRAPHR
                     EPPTPAIGSAQPA"
     gene            complement(4042..5787)
                     /locus_tag="VGA59_05235"
     CDS             complement(4042..5787)
                     /locus_tag="VGA59_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEX9719116.1"
                     /translation="MNPQTSPELRQAMASLWPYFKRAFWFSLVSSLLVLAPTGFMLEV
                     YDRVVNSRSYGTLLMLVLLVLGAFVVMELLEWARSQIMYSASLALDRELGDRIFSAMF
                     TANLRRIPGGTTQSMGDFRVLREFLYSPAVLAAMEAPVSLVFLVLIFAISPMLGWVAM
                     VGALAQTFVGWLNERATRPPLIAANRSAFGAQLYADGTLRNAQVIEAMGMQRHIHRRW
                     IEKQREFLGLQALASERAGGYQAVTKFLQTTLGSTLLGLGAWLLLHNDLRGGAGMMIV
                     ASILGGRLLAPLVQIVTQWRTVVAARDSWGRLNQLLVAVPAPVKPMELPQPKGYLVAE
                     DVVAQAPGGTAQILKGVAFALKPGEVLAVVGPSASGKTTLARVLVGLWPSSGGKVRLD
                     GADVFAWDKAELGPCVGYLPQDVELFDGTIAENIARFGEPEPAKVEAAARAVGLHEYI
                     LELPQGYDSPVGREGAMLSGGQRQRVGLARALYGDPVFVVLDEPNSSLDEAGDSALAA
                     AVLALKARGTTFIVMTHRTSILPVVDKMLVLYEGKRQAFGPRDEVLAALSKSAAQAQQ
                     ANQQRSAQAQVVAAT"
     gene            complement(5784..>6259)
                     /locus_tag="VGA59_05240"
     CDS             complement(5784..>6259)
                     /locus_tag="VGA59_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719117.1"
                     /translation="LHHLAYKSLREQFPQIGSQMVCNAIYSVSLTARIVFQHPASPFH
                     LSRLQGKPLPLLRFLDTSPVYFDRHTLSVKSGALSMYTLDGRMRFMLSLSAADEKNFH
                     EKKLREIVLSQRPKGTFELLFWFTEPQDEEAGARATGAQRPDTRIPDYVKVEAAA"
BASE COUNT         1060 a         2106 c         2043 g         1050 t
ORIGIN      
        1 cagcgaacgc aggtcaggac tgctcaactc ggcgcgctcg gtccaatcct cgaggcgatc
       61 ggggatgggc cgggccagct cgagcttgcg ctcgtccagt ggggccagct ggtcgacggg
      121 catgttcacc atcacctgca actggcgccg cgtgaagtcc acgttctggc gcgcttccag
      181 ctccagggcc agcgtcaggt ccagcttggc ctgggcttca tcgatgtcgg tgcgcacgcc
      241 ggctccggcc gcgaagcgct ttcgcgcggc gtcgaggtgg gtcgtgtaag tctgcttctg
      301 caccatcacc agcgccagct ggtcctgcgc cagcaaagcc tccaggtacg caccggagac
      361 gcgcacggcc acgttttgca cttccttgtc cagcacggcg ttggcctcgg ccacctgggc
      421 ctcggcctgt cgatagtcgg cgatcaggaa gggacggtag atcggctggc gaagggtgag
      481 cgcgcgggtg ccgctcatgt atttctggtc gagcgtcacc ggaactgcgc ccagcccggg
      541 ctgcgtgctc gtgaggtcgt tcttgttgcg ggccaggctg gcggagatat tgggcagcat
      601 ttgcgcccgc gcctgcggaa gccgctcgcg ccgggcgtcg gcggcggcgc gcgccgtccg
      661 gatcaccgcg tcctgctcca tcgcggcgcg ataggcctgg gacaaagtca gcggcgccgc
      721 ggccggcgcg gcggcgggaa ccggtgccgg tccagccggg gcctgcggcc agccggccag
      781 cggggtcatc gccagagcgc acgccaggga cagccgcgcc acgcgccgcc ggggtgccgg
      841 taaatcggac cggttcgatt gcttgaacat cattcctctt tcatggcggc cgcgagccgc
      901 ttgctcaggg ggtgcatcag ataggccagc agcgaacgct cgcccgtcct gaaaacgacc
      961 tcgaccggca tgcccggctg cagcacgcgt ttgcccagtt tcttgtaccc ctccggcgtc
     1021 acgctgaccc gcgacagata gtactggacg ccggattgcg gatcggtgag caggtcgccg
     1081 gaaatggaag ccacctgccc gtcgaccacc aggctgggcg agttcgcgaa cgagttgaag
     1141 cggatatcca ccggcaggcc ggcctgcaca cgatcgatga gattgggagc gaccctggtt
     1201 tcgatcagca gcagctcgtt ctccggaacg atgtccatca gcttctggcc ggactggatc
     1261 acgcccccca cggtctgaac cgccagcccc accacctggc cggcagccgg cgacttgatc
     1321 tcgatgcggc tcaggtcatc cgacagggcg tggaacttgc cctcctcgga ctgaacctcg
     1381 cgcgtcactt ccgtcagctg ggtttcgact tccttgcggt attcctgctg ctttgagatc
     1441 agccgctggc gcagctcccc gatggcgcgc tggcttcgcg tcgtgttccc cagcagctcg
     1501 gcgacggagg agttcgcttc cgcaaccatt cgctcgagtt cgagctggcg gttgcgcggc
     1561 gcgtacccat ccttgaccag gctacgggtg tgcgtcaact cctcatcgag caggcttatc
     1621 tggttacgcc gattgggcag gatgccctga taggactgca gcagggcctc ctggccctgg
     1681 atgccttcct gcaaagcctg cagatcggca cgcagggccg tcctgcgtgc ccggaacaat
     1741 tgctcctggg tctgcatctg gctgcgaata agggggtcct ggctggcact ttgcaggtcg
     1801 gggtggaaaa caatggtgct caaccccgcc tgctccgcaa gcagccggcc ttgcatggtc
     1861 cgcaggctca ggtaacgctg ccggctcgcc tcgtaattgg cccgggcggc ggcttcgtcg
     1921 agtttgagca gcagttgccc ttccttgacc cgatcgccct cgccgaccag gacttccttg
     1981 acgatcccgc cggtggggtg ctggacggct ttgcgcttcg tgttgatcgc caccaggccc
     2041 tggctgggca cgccttcgtc cagcggcgcg agcgctgccc acaccatgaa gccgcccaat
     2101 ccgagcacca gcgcccacag acccacccgg ctcgcacggc ggctggaacc ttccagagcg
     2161 ggcttgacgc ccgcaagcgc aacttccata gaaggcgcgg aatcgtttct ggtcttgagc
     2221 agttgcagtt ctggtttagc catgatggac tcgatcgata tgaggtgcgg aatacggggt
     2281 ggtggcatca agccggctgg gccgaaccga tcgccggggt cggtggttcg cgatggggcg
     2341 cgcgctgcgg cagcaggccg gccagggtag cttcacgcgg gccgtcggcc ttgatccgcc
     2401 catcctgcag gaccatgatc cggtcggcga cggccaccgc accgccccga tgcgtgatca
     2461 ggaacaccgt cttgcccttg gacttgagct gcagcaccgt cttgagcaat gcggcttcgc
     2521 cggcatcgtc gagattggag ttcggttcat cgagcaccag cacggccggg tcgccataga
     2581 ccgcgcgcgc caggccaatg cgctggcgct gcccacccga gagcaggtgg ccgccttcgc
     2641 cgatgggcat gtcgtatccc ttgggaaaac gcaggatcat ttcatgcagc ccggcgcatc
     2701 gggcggcctc gatcaccttg tcggaatcga gttccccgaa acgcgcgatg ttctcgccga
     2761 tcgtgccttc gaacaactcg atgtcctgcg gcaggtagcc caggtgcggg cccagctcgg
     2821 tgcggtccca gccctggatc ggcaggccgt ccagcagcac ctcgccggaa acgtccggcc
     2881 agatgcccat catcacgcgc gccaacgtgg acttgcccga tcccgacggg ccgacaatgg
     2941 ccgtgatggt tcccgcggcg accgaaaaac tgatcccctt gagaatgggt gtggcccggc
     3001 ccggcgcatc ggccacgacc tcgcgcaaga tcacctcgcc ctgcggcgcc acacgcgtca
     3061 ggttgggatc gcgctcggga tactcaacca gcaggcgctc gaggcgctgg aatgccgtcc
     3121 gggtggacag gaagctgcgc cacacggtga ccagctggtc gatcggcgcc agtgcgcgcg
     3181 ccatcagcag gttgacggcg atcatcccgc ccggcgtcaa ctggccatcg atcaccagca
     3241 cggcgccggc cgccagggac agggactgct ggctgtagcg gatgtatttg ctccaggccg
     3301 tcacccgggt gctgacgttc tgcgcctggg tgctcttcgc caggtactcg tcctgcgacc
     3361 ggcgccagcg gctgcgcaga ttgcccagca tgcccatcga ttcgatcacc tcggcattgc
     3421 gcagtttgct ttgcaggtag ccgttgacat cggcgccggc cttggacgcc gcctccgagg
     3481 gcgccaccgt gctgcgatgg ccgaaccatg ccagagcgcc ctggaccacg acaaagacca
     3541 gcgacacgaa gcccagccat gggtgcagga agaaggtgac ggcgatatag atgggcgtcc
     3601 agggcgtatc gaacagggcg aagatgccgg ctccggtgat gaactggcgc agcgtgatca
     3661 agtccgcgaa tgcccggttc gggctggcgg acgactggct cagggacgat tcgaaacttg
     3721 cgttgaacac ccgggtgctg agctgttcgt cgaagcggat gccggccccg accaggattt
     3781 tcgaacgcat ccactccgac accgccatca cgccgaacag gaacagcgtg atcagcgaca
     3841 ccgccaccaa ggtgagctcg ctctggctga ccatcacccg gtcgaagacc tgcagcaggt
     3901 agagcgtggg cgccagcatc atcaggttgg ccaccatgct gaacaggccc accagcagga
     3961 attcgcggcg aaaagcccac aaggcggcgg tcagctcgct gcgttgaaag aaaccaggtt
     4021 tgttcatgga cgtctcggta ttcaggttgc ggccacgacc tgggcctggg cgctgcgctg
     4081 ctggttggcc tgttgcgcct gtgccgccga cttgctcagg gcggccagca cctcgtcgcg
     4141 cgggccgaac gcctggcgct ttccttcgta caacaccagc atcttgtcca ccacaggcag
     4201 gatgctggtg cgatgggtca tgacgatgaa ggtggtgccg cgcgccttga gcgccagcac
     4261 ggccgcggcc aaggccgagt ccccggcttc gtccaggctg gagttcggtt cgtccagcac
     4321 cacgaacacc gggtcgccgt agagggcccg cgccagtccg acccgctgtc gctggcctcc
     4381 cgacagcatg gcgccctcgc ggcccaccgg gctgtcgtag ccttgcggca gctcgaggat
     4441 gtattcgtgc aaaccgacgg cccgggccgc ggcttccacc ttggcgggct cgggctcgcc
     4501 gaagcgggcg atgttttcgg cgatcgtgcc gtcgaacagt tccacgtcct gcggcaggta
     4561 gccaacgcag ggccccagtt cggccttgtc ccaggcgaac acatcggctc catcgagccg
     4621 gaccttgccg ccggaagaag gccacaagcc caccagcacc cgcgccaatg tggtcttgcc
     4681 cgaggccgat ggtccgacaa ccgccagcac ctcgcccggc ttcaaggcga acgccacgcc
     4741 cttcaggatc tgcgccgtgc cgcccggcgc ctgcgccacc acatcctcgg ccacgaggta
     4801 tcccttgggc tggggcagct ccatgggctt gaccggggcc ggcacggcga ccagcaactg
     4861 gttcaaccgg ccccaggagt cgcgcgccgc gaccacggtg cgccactgcg tcacgatctg
     4921 caccagcggt gcgagcaggc ggccccccag gatcgacgcc acgatcatca tcccggcgcc
     4981 gcctcgcagg tcgttgtgca gcagcagcca ggcgcccagc ccgagaagcg tcgagcccaa
     5041 agtggtttga aggaacttgg tgaccgcctg gtagccgccg gcccgttccg aggccagtgc
     5101 ctgcagcccc aggaattcgc gctgcttttc gatccagcgg cggtgaatgt gccgctgcat
     5161 ccccatcgcc tcgatcacct gcgcgttgcg cagcgtaccg tcggcgtaga gctgggcgcc
     5221 gaacgcgctg cggttcgcgg cgatcaaggg cggccgcgtc gctctttcat tgagccagcc
     5281 gacgaaagtc tgcgccaggg ctcccaccat cgccacccag ccaagcatcg ggctgatggc
     5341 gaagatcagc accaggaaca ccagcgagac aggcgcctcc atggccgcca gcaccgcggg
     5401 cgaataaagg aactcgcgca ggacccggaa atcccccatg gattgcgtcg tgcccccggg
     5461 gatgcgccgc aggttcgccg tgaacatcgc ggaaaaaatg cgatcgccca attcgcggtc
     5521 cagcgccaga ctggcggaat acatgatctg cgagcgtgcc cactccagca actccatcac
     5581 cacaaaggcg cccagcacca gcagcaccag catcagcaag gtgccatagc tgcggctgtt
     5641 gaccacgcgg tcgtatacct ccagcatgaa gccggtcggg gccagcacca gcaggctgga
     5701 caccaggctg aaccagaagg cccgcttgaa atagggccac agcgaagcca tggcctggcg
     5761 cagctcggga gacgtttgtg gattcatgcg gcggcctcta ccttcacgta gtcggggatc
     5821 ctggtgtctg ggcgctgggc gccggtcgct cttgcgccgg cctcctcgtc ctgcggctcg
     5881 gtaaaccaga acagcagctc gaatgtgccc ttcggccgtt gcgacagcac gatttcgcgc
     5941 agcttcttct cgtggaagtt tttctcgtcg gccgcggaaa gcgacagcat gaaccgcatg
     6001 cggccgtcga gcgtgtacat cgacagcgct cccgacttca cgctcagcgt gtgccggtcg
     6061 aagtagaccg ggctcgtgtc caggaagcgg agcaagggaa ggggcttgcc ctgaagccgg
     6121 ctgaggtgaa acgggctggc gggatgctgg aatacgatcc gggccgtcaa ggacaccgag
     6181 tagatggcgt tacagaccat ctgggagccg atttgcggaa actgctcgcg cagcgatttg
     6241 taggccaggt ggtgcagcg
//
LOCUS       DASWIY010000138         4524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_450577, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000138 DASWIY010000000
VERSION     DASWIY010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450577"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VGA59_05245"
     CDS             <1..328
                     /locus_tag="VGA59_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX9719118.1"
                     /translation="QLHDLPSGRRVRACGIVTVRQQPQTAHGTIFVTLEDETGPVNVI
                     VWKSLREEQRDDLLKARLLAVEGIWQRDVESGGQVRHLIAHRLKDLTPLLGRLGRQAG
                     GSRDFH"
     gene            complement(346..1563)
                     /locus_tag="VGA59_05250"
     CDS             complement(346..1563)
                     /locus_tag="VGA59_05250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9719119.1"
                     /translation="MKIFYGWRMAAAGAGIQFLHAGLLLQAFGAYVAVLSDELGWSKT
                     ALSGGAALQSIEGALLGPALGWMVDRFGPRVMVEIGIIAFGLGFMALSQIDTLAGFYA
                     AILLIAVGASFSGYFPLSVALVHWFHRRRARALSVMGLGLAAGGLAVPLVGWAMQSHG
                     WRTTAFASGVLAIVVGWPLARILRRRPADIGETADGLPPAPPPDGAGAAEPAGREFSA
                     REALRTRAFWLLALAHGFALLVVSAVNVHAISHMKEGLGYSLAQASLVITLMTVSQAG
                     GVLLGAALGDRWEKRHVAAACMLLHMSGLLMLTYAFHPLMVGAFAVMHGVAWGLRGPF
                     MQAIRADYFGLQAIGMIMGLSAFIISIGQVAGPLVAGVFADLAGNYRGGFTMLAVVAG
                     SGSLLFMLAKKPA"
     gene            complement(1718..1791)
                     /locus_tag="VGA59_05255"
     tRNA            complement(1718..1791)
                     /locus_tag="VGA59_05255"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1757..1759),aa:Gly,seq:ccc)
     gene            complement(1813..4290)
                     /locus_tag="VGA59_05260"
     CDS             complement(1813..4290)
                     /locus_tag="VGA59_05260"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX9719120.1"
                     /translation="MFAKSLKAIAALIALLLIAAAVYVWRSFPQLDGEMRATGLAAAV
                     QVRRDASDVTHIQAQSIADAYFALGWVHAQERSWQLEFNRRLMHGELSEVLGAATLET
                     DKLLRTLGIMRSAQAQWERLPGDAKALLQAYANGINGFHAEAGQALPPEFHILGTRPG
                     TWTPQDTMGWALMMALDLGGNWGNEFARLSALQRIDTATLWQLMPPYPGEPPATAVDL
                     AALYRGLGVYRNGEPAATRTSAVPDEALPARGPVLVDAQINDWARHLGQPDGKGSNNW
                     VVAGTHTESGKPLLANDPHLALASPAIWYFARLQAPASAGGKAMDVIGATLPGLPSVV
                     LGRTAGVAWGYTNVGPDVQDLYLEQIDPADPARYRTPDGWARFDTREEVIKVKGQPDA
                     SIVVRGTRHGPVLSDVQAIHGQVLDTRKFVIALRWTALDAGNQTVLSGVRSNAAQTVD
                     ELMTAFANYHSPMQNVVAADTHGRTAYRAIGRAPLRKVENDLRGVAPAPGWESRYDWV
                     GELAADEYPHAGHEAIQSKGWHATANQRIHPAGHAHFLGSDWLTPERFDRIESLLGAK
                     PRHTAMSMRDVQADILSTAARRLLPLLQATQSSHALAPAALAQVKAFDGVMRADGAAP
                     LIVSVWADELTRGLVEPRLGPEKFQVLYGKRSFRQGLETILADAKAAASWCAPLSCAE
                     QSARALERSLDRIAAQQGKDPAAWRWGPWHSALSAHRPFGNVAALAGVFDVAVPTGGD
                     SWTVNVGQYWANDPRVPFANRHAASMRAIYDLADLEKSQFIYQTGQSGLVFSPRYRDM
                     KDQWAAVQYRPLRLQPDRWVHEAKLVP"
BASE COUNT          741 a         1546 c         1561 g          676 t
ORIGIN      
        1 gcaattgcac gaccttccca gcggccggcg cgtgcgcgcc tgcggcatcg tcaccgtgcg
       61 ccagcagccg caaacggcgc acggaaccat cttcgtcacg ctggaggacg agaccgggcc
      121 ggtcaacgtg atcgtctgga agtcgctgcg cgaagaacag cgcgatgacc tgctcaaggc
      181 gcggctgctg gccgtcgaag gaatctggca gcgcgacgtg gaaagcggcg ggcaggtgcg
      241 ccacctgatc gcccaccggc tcaaggactt gacgccgctg ctggggcggc tgggacggca
      301 agcaggcggc agccgcgact ttcactgacc ccggtcgcgc gacgctcagg ccggtttttt
      361 cgccagcatg aacagcagcg agcccgatcc cgcaacgacc gccagcatgg tgaagccgcc
      421 gcggtagttg ccggcgaggt ccgcgaagac acccgcaacc agcggcccgg ccacctgccc
      481 gatggagatg atgaaagcgg acaggcccat gatcatgccg atcgcctgga ggccgaaata
      541 gtcggcccgg atggcctgca tgaaggggcc tcgcaggccc caggcgacgc cgtgcatgac
      601 ggcgaacgcg cccaccatca gcgggtggaa ggcataggtc agcatcagca ggccgctcat
      661 gtgcagcagc atgcaggcgg cggcgacatg gcgcttttcc cagcggtcgc ccagcgccgc
      721 gccgagcagc acgccgcccg cctgcgacac ggtcatcagc gtgatgacga gcgaggcctg
      781 cgccaacgaa tagcccaggc cttccttcat gtgcgatatc gcgtgcacgt tgacggcgct
      841 caccaccagc agcgcgaagc cgtgcgccag cgccagcagc cagaaggcgc gtgtgcgcaa
      901 cgcctcgcgt gcgctgaact cgcgcccggc cggttccgcg gcgccggccc cgtccggcgg
      961 cggcgcgggc ggcagtccgt cagccgtttc gccgatgtcg gccggccggc ggcgcagtat
     1021 tcgcgccagc ggccagccca ccacgatggc gagcacaccc gaagcgaaag cggtggtgcg
     1081 ccagccgtgg ctttgcatgg cccagcccac cagcggcacg gcgagaccgc cggcagccag
     1141 gcccaggccc atgacggaca gcgcgcgggc acgccgccgg tggaaccagt gcacgagtgc
     1201 caccgacagc ggaaagtaac ccgagaagct cgcgcccacg gcgatcagca ggatcgccgc
     1261 gtagaagccg gcaagggtgt cgatctggct gagggccatg aatcccaacc cgaacgcgat
     1321 gatgccgatc tccaccatca cgcggggccc gaatcggtcg accatccagc ccagcgcggg
     1381 accgagcagc gcgccctcga tcgactgcag cgcggcgcct ccggacaggg ccgtcttgct
     1441 ccagccgagt tcatcggaaa gcaccgccac gtaggcgccg aaggcctgca gcagcaggcc
     1501 ggcgtgcagg aactggatcc ccgcgccggc ggccgccatc cgccagccat agaaaatttt
     1561 caaacctgcg cctgcttcga aatcggatca gtttagcggc gcacgcaaga tgaatacgcg
     1621 agttctaagc gtgagagcgc tcgccgctgc actcgcaacg gccgcgagcc gggttcgtgc
     1681 ttgagggaaa acgcaggcgt tgaatcacgc gcgcagatgg agcgggtgaa gggaatcgaa
     1741 ccctcgtcta aagcttggga agctgccgtt ctgccattga actacacccg cgggagctga
     1801 gcattctacc tgctacggca ccagcttcgc ttcatgcacc cagcggtcgg gctgcaggcg
     1861 tagcggccgg tactgcacgg ccgcccactg gtccttcatg tcgcgatagc gcgggctgaa
     1921 caccaggccg ctctgccccg tctggtagat gaactgggac ttctccagat ccgccaggtc
     1981 gtagatggcc cgcatgctcg cggcatgacg gttggcgaag ggcaccctgg ggtcgttggc
     2041 ccagtactgg ccgacgttga ccgtccagct gtcgccgccg gtgggcactg cgacgtcgaa
     2101 aacacctgcc agcgccgcca cgttcccgaa cggcctgtgg gcggacaacg ccgaatgcca
     2161 gggtccccag cgccaggcag cggggtcctt gccctgctgc gcggcgatgc ggtccagcga
     2221 gcgttccagg gcgcgcgcgg actgctccgc gcagctgagg ggggcgcacc atgaggcggc
     2281 cgcctttgcg tcggccagaa tggtttcgag tccctggcgg aaagagcgtt tgccgtacaa
     2341 cacctggaat ttttccgggc caagccgcgg ctcgacgagg ccgcgcgtga gctcgtcggc
     2401 ccagaccgag acgatcagcg gcgctgcgcc gtcggcacgc atcacgccat cgaatgcctt
     2461 gacctgggcc agcgccgccg gtgccagcgc atggctgctt tgcgtcgcct gcaggagcgg
     2521 cagcagcctg cgcgcagctg tcgagaggat gtcggcttgc acgtcgcgca tgctcatcgc
     2581 cgtatgcctg ggcttcgcgc ccagcagcga ttcgatgcgg tcgaagcgct cgggcgtgag
     2641 ccagtcgctg cccaggaaat gcgcatggcc ggctggatgg atgcgttggt tggccgtcgc
     2701 atgccagccc ttgctctgga tggcttcgtg gccggcgtgc gggtactcgt cggccgcgag
     2761 ttctccgacc cagtcgtatc ggctttccca tcccggagcc ggcgcgacgc cacgcaggtc
     2821 gttttcaacc ttgcgcaagg gtgcgcgccc gatcgcccgg taggcagtgc ggccatgggt
     2881 atcggccgcg acgacgttct gcatcgggga gtggtagttg gcaaaggcgg tcatcagctc
     2941 gtcgaccgtt tgcgcggcgt tcgatcgcac gccggacagc acggtctggt tgcccgcatc
     3001 gagggcggtc cagcgcagcg cgatcacgaa cttgcgtgta tccagcacct ggccgtggat
     3061 ggcctgtacg tcgctcagca ccggcccatg ccgcgtgccg cgcaccacga tgcttgcgtc
     3121 gggctgtccc ttgaccttga tgacttcctc gcgcgtgtcg aagcgcgccc agccatcggg
     3181 cgtacggtac cgcgcggggt cggccgggtc gatctgctcc aggtagagat cctgcacgtc
     3241 ggggccgacg ttggtgtagc cccacgccac ccccgcggtg cggcccagga cgacggaggg
     3301 caggcccggc agcgtcgccc cgatcacgtc catggccttg ccgccggcgg acgcaggcgc
     3361 ctgcaggcgc gcgaagtacc agatggcggg agaggccaac gcgaggtgcg ggtcgttggc
     3421 gagcaatggc ttgccgctct cggtatgggt gcccgcaacc acccagttgt tggaaccttt
     3481 gccatcgggc tggcccaggt ggcgggccca gtcgttgatc tgcgcatcca ccaggaccgg
     3541 gccgcgcgcc ggcaaggcct cgtccggcac cgcgctcgtg cgcgtggcgg cgggctcccc
     3601 gttgcggtag accccgaggc cccggtacaa cgcggccagg tcgacagccg tcgccggcgg
     3661 ctcacccgga taggggggca tcagctgcca cagggtggcg gtgtcgatgc gctgcaatgc
     3721 cgacagccgc gcgaactcgt tgccccagtt gccgcccagg tccagcgcca tcatcagcgc
     3781 ccagcccatg gtgtcctgcg gcgtccaggt gcccggccgg gtgccgagga tgtggaactc
     3841 gggcggaagg gcctgcccgg cctcggcatg aaagccgttg atgccgttcg cgtacgcctg
     3901 caggagggcc ttcgcgtcgc cgggcagacg ctcccactgc gcctgggccg atcgcatgat
     3961 gcccagtgtg cgcagcagct tgtcggtttc cagcgtagcg gcgccgagca cctcgctcag
     4021 ctcgccgtgc ataagacgcc gattgaattc gagctgccag cttcgctcct gcgcatgaac
     4081 ccagcccagc gcgaaatagg cgtcggcgat gctttgcgcc tggatatggg tgacgtcgct
     4141 cgcgtcgcgg cgcacttgca ccgccgcggc caggccagtc gcccgcattt cgccatcgag
     4201 ttgcggaaag ctgcgccaca cgtacaccgc cgccgcgatc agcagcaacg cgatcagggc
     4261 cgcgatggcc ttcagggact tggcgaacat ctggtttcct ctggtgctgc cggtgcgctc
     4321 gagcggatga atgtaccgcg ccagtgccgc ggccgtccag gcgcggcagc ggcaggcgtg
     4381 cctcctgagt atgagaaaaa aattgcccag cagtgcggcc gggcaatcag gcagtcggtt
     4441 ggcggcgcac gaagcgcgtg aacaattgcg gccagtgcgg ggccaccgcg gaaccggctt
     4501 tgccgggccg ctggtgggcc ttgc
//
LOCUS       DASWIY010000139         4688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2672977, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000139 DASWIY010000000
VERSION     DASWIY010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2672977"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(396..1667)
                     /locus_tag="VGA59_05265"
     CDS             complement(396..1667)
                     /locus_tag="VGA59_05265"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9719121.1"
                     /translation="MALAACVVPAGNPDDAALDRLIDALPQCQREPEYLAALGNILNQ
                     RGRYAEAGDHLERALMLAPDLKGARLDYAIALAGIGEVESARLLLDDLLADPGLPPHL
                     KPVLERQRRNWASTTDWQTRVLVSGRLGYDSNLLGSPDLSALTLTFPDQSVVLPLDES
                     ARANAGSYARLDLQFEARKLTLWGGQFESFLGLRSRTSGAVSDADSQQADAVVDYSSY
                     RRSNNGGGYYTGASASVLRSGSGIRYNAYGLAAGIGSSGWVAGCEARIGLEIQERKYV
                     NNDLLSGRYSGIASSLSCDRRGIQWLASAKAGIDRASHADRAGGDQAQYALRGAVVFS
                     SVGPGRFAKGRLWLDVELNRSRDSSGYSPLLESGRLRIIERASTRIEYQYPLSNSLQW
                     VAGAERVLQHSSLALFANRSWGPYLALRGAW"
     gene            complement(1714..3591)
                     /locus_tag="VGA59_05270"
     CDS             complement(1714..3591)
                     /locus_tag="VGA59_05270"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9719122.1"
                     /translation="MAIACLAWIGQAYVAPRWLADLEARSGDVVWRLNAQRKDERRLI
                     IVDIDEGSLREVGPWPWPRSTQARLVEEIARLGADQQIFDIVFTDQRNGDAELAAAIR
                     QHNSVLAQVFALEQGGEPSVGRLGGALDWTACPAPFGEAQGFLANTAVLRSDFVGHIT
                     PRLDSDGLVRHVPAVICHQGKAYPALALAALMRATSEQGLVLQKGKAWLDAPWTLTGK
                     NLPDAIPLDRRGDMRVPWRLHPQSFVSLSAADVLAGRVPAGLLEKAWVLVGSTAFGLN
                     DTVATPFGAAGSGLQVHAELMAGLVDGRPPYTPRLAPWYQALAALAGVVLLVFLGREG
                     RRFPVYLLPLAALGWCAVLWLVHAVALDRASLWMGWASPAFFVLVAGAALGILQHAKS
                     RIDRDRLFTHLSSYLPAPVAAALVSQSPSSAIKASTNQVSVMFADIRNFSAYCEARPP
                     EEAAAVLHAFFSTATRVVESEGGVIEAFQGDAILAVWNGDPAPNSATAAATDHAASAL
                     RAAVKLNAAIQGVLPDPAPAGLEPLALGIGVETGPAMAGSFGLASRRTHLVLGRTVTI
                     ASRLVEMTADLAHPILVGEGLAAHVGAKGLESLGTFLLDGMRVPHHVYAYPLAASVRL
                     D"
     gene            complement(3643..>4688)
                     /locus_tag="VGA59_05275"
     CDS             complement(3643..>4688)
                     /locus_tag="VGA59_05275"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX9719123.1"
                     /translation="AIKFRLDEGVVRSITGSWGEAARDKFRLNTPVAAIGVKGTDFVV
                     RSDAEKTNASVYTGAIVLSPLTAGCVATLGPCQSGREKLLSDDMKGQMLELSRQQASP
                     QLVPLVDLLAQNRRVVPGSDSTRVEKTVVTDASRPDFASDKEVVTEALSADVVPAGST
                     RPPSAPPAPPPIADAPLPPVVTQLVWGRWDWTVASAGETFSRTFEEAAQNGRRITVGN
                     GAYALYRTAPHANSLLRTDEASANFRLSAGAGQLSVPGQASQDVRVDGGSLNVDFSRA
                     TFATQLNVSNPTIGPNTLQSNGSVSPKGVMVGQSGNAYVAGALSLDGKEAGYFFEKAF
                     SAGNLSGITLWGR"
BASE COUNT          830 a         1569 c         1556 g          733 t
ORIGIN      
        1 caccgaggac gtcggcgaac gctacgtcgc tggtcgcctg aatcgtcgca acccagttgc
       61 tatagacggg gaatgtgggt gcaccaccta caaacgccat gtgcaactgg acggcagtcg
      121 cttgcgggcc aaaaattgaa acagccatgt ttcttcctct aaaggttaat ggagaactgg
      181 aaccggtatc gaacgggagc ttaaacgctt gcctttgtga aagacacgca ttcaaacccc
      241 acccttatca agttcgctgg gcattatcac atgcagaatt ccacgagccg catgttctgt
      301 tgccttttgc caacttgatc gcagtgtagg gactgcgcat cgcgcaaaca agtgaccgcc
      361 atcacatggc ttcgcatgtt gtgcggcctc gcaagctacc aggcgccgcg caatgcaagg
      421 tacggccccc agctgcgatt ggcgaaaagc gcaaggctgg aatgctgcaa tacccgctcg
      481 gcgcccgcca cccattgaag gctgttggac aacgggtatt ggtactcgat gcgggtgctc
      541 gcccgttcaa tgatgcgtaa gcgcccactc tccagcaaag gactgtagcc gctgctgtcg
      601 cgcgatcgat tgagctcgac atccagccac agccggccct tggcgaagcg gccgggaccc
      661 acggacgaaa agaccaccgc gccccgcagc gcatattgcg cctggtcgcc gccggcgcgg
      721 tccgcgtgac ttgcccgatc gatgcccgcc ttcgcactgg ccagccactg gatgccccgg
      781 cgatcgcacg acaaggaact tgcgatgccg ctgtagcgtc ctgacaacaa atcgttgttc
      841 acgtacttgc gttcctggat ttccaggcca attcgggcct cgcaacccgc gacccagccc
      901 gaacttccga tgcccgcggc aagaccgtat gcgttatagc ggatgcccga accggagcgc
      961 aggacggaag cggacgcgcc ggtgtagtaa cccccgccgt tgttgctgcg ccggtagctg
     1021 ctgtaatcca ccaccgcatc ggcctgctgg gaatcggcgt cggaaacggc gccgctggtg
     1081 cggctgcgca gaccgaggaa ggactcgaac tggccccccc agagcgtgag ctttcgcgct
     1141 tcgaactgaa ggtccagccg cgcgtaagaa ccggcgttgg ctcgcgcgct ctcgtccagc
     1201 ggcagtacca cggactggtc ggggaaggtc agcgtcaagg cactgaggtc cggggatccg
     1261 agcagattgc tgtcgtagcc gaggcgccca ctcaccagca ccctcgtctg ccaatcggtg
     1321 gtcgaggccc agttgcggcg ctggcgctcc agcacgggct tcaggtgcgg cggcaggcct
     1381 ggatcggcca gcagatcatc cagcagcagc ctggccgatt cgacttcccc tatgcccgcc
     1441 agcgcgatgg cgtaatccag gcgcgcgccc ttcaggtcgg gtgccagcat cagggcccgc
     1501 tccaggtgat cgccggcttc ggcgtagcgg cctcgctgat taagtatgtt gcccagcgcc
     1561 gccaggtact cgggctcgcg ctggcactgg ggcaaagcgt cgatcaggcg atcgagtgcc
     1621 gcatcgtcgg gattgcccgc aggcaccacg cacgcggcca gcgccatgcc cggccccaga
     1681 aggcagacga gcgcgacagc acggactgtc cttctaatcc aatctgactg aagcggcaag
     1741 cggatatgcg tatacatgat gcggcacgcg catgccatcg agcaggaacg tgccaaggga
     1801 ttcgagtccc ttggcaccca catgggcggc caggccctcg ccgaccagga tcggatgggc
     1861 cagatcggct gtcatctcca ccaggcggct ggcaatggta accgttcgcc ccagcaccag
     1921 atgcgtgcgc cggctggcca ggccgaacga gcccgccatg gcgggtccgg tttcgacgcc
     1981 gatccccagc gcgagcggtt ccaggccggc gggtgcgggg tcggggagca ccccctgaat
     2041 ggcggcattg agcttgacgg ccgcccgcag ggcgctggcc gcgtgatcgg tcgcagccgc
     2101 ggtggcggaa ttgggcgccg ggtcgccgtt ccacacggcc aggatggcgt cgccctggaa
     2161 cgcctcgatc acgccgccct cggactcgac gacccgggtg gcggtggaga agaacgcgtg
     2221 cagcaccgcg gcggcctctt cgggcggccg ggcctcgcaa taggcggaga agttgcggat
     2281 gtccgcgaac atgaccgaca cctggttggt gctggccttg atcgcgctgg agggcgactg
     2341 cgagaccagg gcggccgcca ccggcgcggg cagatagctc gaaaggtgcg tgaacagccg
     2401 gtcgcggtcg attcggctct tcgcgtgctg caggatgccc agcgcggcgc cggccacgag
     2461 gacgaagaag gccgggctgg cccaccccat ccagagtgac gctcgatcca gcgcgaccgc
     2521 atggaccagc cacagcacgg cgcaccaccc cagcgcggcc aagggcagca gatacacggg
     2581 aaaacgcctg ccctcgcggc ccaggaagac cagcaggacc acgcccgcca gcgcggccag
     2641 agcctggtac caaggcgcca accgcggcgt atagggtggc cggccgtcga ccaggcccgc
     2701 catgagctcg gcgtgaacct gcaaccccga accggccgcg ccgaagggtg ttgcaacggt
     2761 gtcgttcagc ccgaacgcgg tgctgcccac caggacccag gccttctcga gcagccccgc
     2821 gggcacgcgc ccggccagga cgtcagccgc ggacaggctc acgaagctct gcgggtgcag
     2881 ccgccacggc acccgcatgt cgccacgccg gtccagcggt atcgcatccg gaaggttctt
     2941 gccggtcagc gtccacggcg catcgagcca ggctttgccc ttctgcaaca ccagaccttg
     3001 ctcggaagtg gcgcgcatga gtgccgccag ggcaagagcg gggtaggcct tgccctggtg
     3061 gcagatcacc gccggcacat ggcgaaccag cccgtcgctg tccagcctgg gggtgatgtg
     3121 cccgacgaaa tcgctgcgca gcaccgccgt gttggcgagg aagccctggg cctcgccaaa
     3181 gggagccggg caagcggtcc agtcgagggc gccgccgagc cggcccaccg acggctcgcc
     3241 gccctgctcc agggcgaaga cctgcgccag gacagaattg tgctggcgga tggcggcggc
     3301 caattccgca tcgccgttgc gctgatcggt gaagacaatg tcgaagatct gctggtcggc
     3361 gccgagccgc gcgatctctt ccaccaggcg ggcctgcgtg gaccgcggcc agggccaggg
     3421 acccacttcg cgcaggctgc cttcgtcgat gtcgacgatg atcagccggc gttcgtcctt
     3481 gcgctgcgcg ttgagccgcc agacgacatc acccgaccgg gcttcgaggt cagccagcca
     3541 gcggggggca acgtacgcct ggccaatcca ggccaggcaa gcgattgcca acccgaagcc
     3601 gacaacccaa tgtgtgccga gtgatctcca gaaccgggtc aatcagcgtc cccaaagggt
     3661 gattccgctg agattgcctg cagagaatgc tttttcgaag aagtaaccgg cctctttgcc
     3721 atccagactg agggctcccg cgacataggc gttgccgctt tgtcccacca tgacgccttt
     3781 gggactgacg ctgccgttgg actgaagggt gttggggccg atcgtcgggt tcgacacgtt
     3841 caattgtgtc gcgaacgtcg ctcgcgaaaa atcgacgttc aggctgccgc cgtccacgcg
     3901 gacgtcctgg ctggcctgcc cgggcacgga taactggccc gcgccggcgc tgagccggaa
     3961 attggccgag gcctcgtccg ttcggagcag cgagttggca tgcggcgccg tgcggtaaag
     4021 cgcgtaagca ccgtttccca ccgtgatgcg gcggccattc tgggccgctt cctcgaaggt
     4081 gcgcgaaaag gtctcgcccg ccgaggcgac ggtccagtcc cagcgccccc agaccagttg
     4141 ggtgaccacg ggagggagcg gggcgtcggc gatcggcggc ggcgccgggg gagcggacgg
     4201 cgggcgcgtg ctgccggccg ggacgacgtc ggccgacagg gcttcggtca ccacctcctt
     4261 gtccgacgcg aagtccgggc ggctggcgtc ggtcaccacc gttttttcga cccttgtcga
     4321 gtcggagccc ggcaccacac gccggttctg ggccagcaga tcgaccagcg gcaccagctg
     4381 cgggctggcc tgctggcggc tcagttcgag catctgcccc ttcatgtcgt cggacaacag
     4441 cttttcgcgg ccgctctggc agggaccgag cgtcgcaacg cagccggcgg tcagggggga
     4501 cagcacgatg gcgccggtgt aaaccgaagc attcgttttt tcggcgtccg acctgaccac
     4561 gaagtccgtt cccttgacgc cgatggcggc cacgggcgtg ttgaggcgga acttgtcgcg
     4621 cgcagcctcg ccccatgagc ccgtgatgga gcgcaccacg ccttcgtcga gacggaactt
     4681 gatggccg
//
LOCUS       DASWIY010000140         4282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1183635, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000140 DASWIY010000000
VERSION     DASWIY010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183635"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VGA59_05280"
     CDS             complement(<1..818)
                     /locus_tag="VGA59_05280"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX9719124.1"
                     /translation="MMILRAALLALLTLTALAAAPAYADDNRPLFVEINEQAPLEYRL
                     RWKVPANVPASISPALVLPTGCTPQGRMRQWSDMLGHWQEGRWNCAQSLRGAEIGIDY
                     PGANPALATIARIRWADGEEQTHLSLPGVAAIPLAEATPAAGFLDYVKLGFEHIWLGL
                     DHLLFVAGLIWIARTWKRIALAITGFTVAHSITLALAALDVIRLPIAAVEGVIALSIV
                     FLAIEIVKGPRDTLTWRRPVAVAGSFGLLHGLGFAAVLREIGLPQEGFVSALLG"
     gene            complement(815..1816)
                     /locus_tag="VGA59_05285"
     CDS             complement(815..1816)
                     /locus_tag="VGA59_05285"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9719125.1"
                     /translation="MRAVTFQALHRPLTVDTVPDPVPGEGEVVVKVGRCGICGSDLHM
                     TEDPAYGCKHGDVLGHEFAGEVVGLGKDVDGLKTGDLVSVIPLKSCGKCEHCRKGEVQ
                     WCAEFGLQGGGYAEYAVTRPNQCIRLPADLSLADGAIVEPLAVALHGLNLSGMKAGDK
                     VLVLGAGPIGLAVAFWAKRLGAAKVAVQDIAEFQRDRALGMGADVFVVDPEDPVGSAE
                     RGLGGKADIVFECVGIPGLIARAVDQVKPRGTILLLGLCTQPDTFNSFAMLSKEVKLV
                     TSAFFTRQEYEAALDALDEGAAEPRLLVTDTISLSDTPGVFESLKRRTEQCKVLIAP"
     gene            complement(1818..2708)
                     /locus_tag="VGA59_05290"
     CDS             complement(1818..2708)
                     /locus_tag="VGA59_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9719126.1"
                     /translation="MRYLQMIVAALAAAFMAQPALARDTPLKVEVLRTSAGSLHVNSA
                     LIFGEKDAVLIDPPFTKADAHRVTAMVLDSGRRLTHIFVTHDHPDHFFSMDVLADAFP
                     DAQIVAHPQVVDAIWRSLPFKVKRWSAVLGPNGPASPSAPRPLSGDTIMLEGQELVVV
                     GPMWGDHAVSTALWAPGIKALFPGDLVFNQMHLWLGEHRPEDIAAWHRSLVELARLEP
                     ELVVAGHALPGLANDASGIEFTLSYLEAWPGLVAVSKDSKDLQARIRAAFPDAIDVLD
                     DFILVNSTEVAMGEQPIWTE"
     gene            complement(2729..3850)
                     /locus_tag="VGA59_05295"
     CDS             complement(2729..3850)
                     /locus_tag="VGA59_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX9719127.1"
                     /translation="MSTQAQAAICRGSSTPFTIEEVMLDALRPDELRIRVVACGVCHT
                     DMAVRDQQLPVPLPVVLGHEGAGIVEEVGSEVTVARPGDRVVMSFNSCGHCPSCDIDA
                     PTYCYDFFPHNWSGKRVDGSPTMFLDGEPMNANFFGQSSFATHVIAHERNTVRVPDSA
                     ASVPLERLAPIGCGLMTGAGAVLRAMKVRSGLPIAVFGAGTVGMAAIMAAKIAGANPI
                     IAVDVNDERLAMAKELGATRAFNAKDDAVGRIRELCPPGLGYAFDTTGINKVIEDAWN
                     LLAPKGICGIVGASDPADNLTFNEAAFMGGGRTVMGILGGDSDLTNFLPELVEHHLAG
                     RFPFEKLIGYFDFSEINEAIHASESGKVVKPVLRIGAEN"
     gene            complement(3884..>4282)
                     /locus_tag="VGA59_05300"
     CDS             complement(3884..>4282)
                     /locus_tag="VGA59_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9719128.1"
                     /translation="ARGGRRGGSIVVTSSNAAEVNEAIVGVPYMAAKAGVKHFMRHAA
                     YELAAFGIRVNAIAPGPFVTNIGDGWVKKNPQAKLAWDKLVPVGRMAETWQIKPLALL
                     LASDAGSYMTGSHVMIDGGMQLGPVKPLNE"
BASE COUNT          766 a         1385 c         1367 g          764 t
ORIGIN      
        1 aagcccagca gggcagagac gaaaccttcc tgtggcaggc cgatctcgcg cagcactgcg
       61 gcgaagccga gcccgtgcaa tagtccgaaa ctgccggcca ccgccaccgg ccgccgccag
      121 gtcagtgtat cgcgcgggcc cttcacgatc tcgatggcga gaaacactat ggaaagcgcg
      181 attacaccct cgaccgccgc aataggcaag cggatcacgt cgagcgcggc gagcgccagg
      241 gtgatcgaat gggcaacggt gaaaccggtg atggcgagcg cgatccgttt ccaggtccgc
      301 gcgatccaga tgagtccggc gacgaacagc aagtggtcga gcccgagcca gatatgttcg
      361 aagccgagct tcacataatc gagaaagccg gcggccgggg tggcttcggc cagcgggata
      421 gcggccacgc cgggcaggct gagatgggtc tgttcctcgc cgtctgccca gcggatgcgg
      481 gcgatggtgg cgagcgccgg gtttgcgccg ggataatcga tgccgatttc cgccccgcgc
      541 agcgattgcg cgcagttcca gcggccctcc tgccaatggc cgagcatgtc ggaccattga
      601 cgcattcggc cttgcggcgt gcagccggtt ggcaggacca gcgcgggcga gatgcttgcg
      661 ggaacattgg cgggcacttt ccagcgcagc cggtattcaa gcggcgcctg ctcgttgatc
      721 tcgacgaaga gcggacggtt gtcgtcggca tacgcgggcg ctgcggcgag ggccgtcagc
      781 gtgagcaggg ccagcaacgc ggcgcggagg atcatcatgg cgcgatcagt accttgcatt
      841 gctcggtgcg gcgcttgagg ctctcgaaca cgccgggcgt gtcggacagc gagatcgtgt
      901 cggtcaccag caggcgcggc tcggcggcgc cttcgtcgag cgcgtcgagc gcggcttcat
      961 attcctgccg cgtgaaaaag gcgctggtga cgagcttcac ttccttcgag agcatggcga
     1021 agctgttgaa cgtgtcgggc tgggtgcaca agccgagcag caggatcgtg ccgcgcggtt
     1081 tcacctggtc gaccgctcgt gcgatcagcc cgggtatgcc gacgcattcg aagacgatgt
     1141 cggccttgcc gccgagccct cgttcggcgc ttccgaccgg atcctcggga tcgaccacga
     1201 agacgtccgc acccatgccg agcgcgcggt cgcgctggaa ttcggcgatg tcctgcaccg
     1261 ccactttggc cgcgcccagg cgcttggccc agaaggcgac ggccaggccg atcggccctg
     1321 cgcccagcac aagcaccttg tcgcccgctt tcatgcctga aaggttgagg ccgtgcagtg
     1381 ctacagccag cggctcgacg atcgctccat cggcgaggct caggtcagcc ggcaaccgga
     1441 tgcactggtt gggccgcgtc actgcatatt cggcatagcc gccgccttgg aggccgaact
     1501 cggcgcacca ttgcacttcg cccttgcggc aatgctcgca cttgccgcag ctcttcagcg
     1561 ggatgacgga aacgagatcg cccgttttca ggccatccac atccttgccc agccccacaa
     1621 cttcgccggc gaattcatgg cccagcacgt cgccgtgctt gcagccatag gccgggtctt
     1681 cggtcatatg caggtcggag ccgcagatgc cgcagcggcc gaccttgacg accacctcgc
     1741 cttcgcccgg cacaggatcg ggcacggtgt caaccgtcag cggcctgtgc agcgcctgga
     1801 aggttacggc gcgcatttca ttcggtccag atcggttgtt cgcccatcgc tacttcggtg
     1861 gaattgacga ggatgaagtc atccagcacg tcgatcgcat cggggaaggc tgccctgatc
     1921 cgcgcctgaa gatcctttga atccttcgaa acggcgacga gccccggcca cgcctcaaga
     1981 taagagagag tgaattcgat gccgctggca tcattggcca ggccgggcag ggcatgtccg
     2041 gcgacgacca gctccggttc gagcctggcc agctccacca gcgagcggtg ccaggcggcg
     2101 atatcctctg ggcggtgctc acccagccag agatgcatct ggttgaatac aagatcgccg
     2161 ggaaacagcg ctttgatgcc gggcgcccac aatgcggtcg aaaccgcgtg atcgccccac
     2221 atcgggccca ccaccacgag ttcctgcccc tccagcatga tcgtgtcacc tgaaaggggc
     2281 cggggcgcgg aaggactggc tgggccgttg gggccaagca ccgcgctcca gcgcttcacc
     2341 ttgaatggca gcgaacgcca gatcgcatcg accacttgcg gatgcgccac gatctgagca
     2401 tcggggaagg catcggccag cacgtccatc gagaagaaat ggtccggatg atcgtgcgtc
     2461 acgaagatgt gcgtcagccg cctgccgctg tcgagcacca tggcggtgac gcggtgcgcg
     2521 tccgcctttg tgaagggcgg atcgatcagc acagcatcct tttctccgaa gatgagcgct
     2581 gaattgacat gtagcgatcc agcgctggtt cgcagcactt ccactttcag gggtgtgtct
     2641 cgggcgaggg cgggctgggc catgaaagcc gcagccagcg cggccacgat catctgtagg
     2701 taacgcatct atattcccct agacttgctt agttttccgc acctatccgc agcacgggct
     2761 tcaccacctt gccgctttca ctggcatgga tcgcttcgtt gatctcggaa aagtcgaaat
     2821 agccaatcag cttctcgaat gggaaacggc ctgccaggtg gtgttccacc agttccggca
     2881 agaaattggt aagatcgctg tcgccgccca gtatgcccat cacggtacgc ccgccgccca
     2941 taaaggccgc ttcgttgaag gtgaggttgt ccgccggatc gctcgcgccc acaatgccgc
     3001 agatgccctt gggcgccagc aggttccatg catcttcgat caccttgttg atgccggtgg
     3061 tatcgaaagc atagccaagc ccgggcgggc aaagctcgcg gatcctgcca acggcgtcgt
     3121 ccttcgcgtt gaaggcgcgg gtcgcgccca gttccttcgc catggcgagc cgttcgtcgt
     3181 tcacgtccac cgcgataatc gggttcgcgc ctgcgatttt cgccgccatg attgcggcca
     3241 tgcccaccgt gcctgcgccg aacaccgcga tcggaagccc cgagcgcacc ttcattgcgc
     3301 gcagcaccgc gccggcccct gtcatcagcc cgcagccgat cggcgcgagc cgctcgagcg
     3361 gaacggaggc cgcgctgtcg ggcacgcgca ccgtgttgcg ttcatgcgca atgacatgtg
     3421 tggcgaagct ggactggcca aagaaattgg cattcatcgg ttcgccgtcc aggaacatgg
     3481 tgggcgatcc gtccacccgt ttgcccgacc agttatgcgg gaaaaaatcg tagcaatagg
     3541 tcggtgcgtc gatatcgcag ctggggcaat ggccgcagga attgaagctc atcaccaccc
     3601 tgtcgccggg cctggcgacg gtgacttcgc tgccgacttc ctccacgatc ccggcgcctt
     3661 catggcccag cacaacgggc aacggcacgg ggagctgctg gtcgcgcacg gccatatctg
     3721 tgtgacacac tccgcatgcc accacgcgga tgcgcagctc atcgggccgc agcgcgtcga
     3781 gcatcacttc ctcgatggtg aagggcgtgg aactgccgcg gcaaatcgcg gcttgggcct
     3841 gcgtgctcat agtttcctct caagttccat ggccgtagcg gcgctattcg ttcagcggtt
     3901 tcaccggccc cagctgcatg ccgccatcga tcatcacatg gctgccggtc atgtagctgc
     3961 cggcatcgga ggcgagcagc agggcgagcg gtttgatctg ccaggtttcg gccatgcgac
     4021 cgaccgggac cagcttgtcc catgccagtt tcgcctgtgg gttcttcttg acccagccgt
     4081 cgccgatatt ggtcacgaac ggccccgggg cgatggcgtt gacgcggata ccgaacgcgg
     4141 cgagctcgta ggcggcatgg cgcatgaaat gcttcacgcc tgccttggcc gccatatagg
     4201 gcacacccac gatcgcctcg ttgacttcgg cggcgttgga gctggtgacg acgatggaac
     4261 cgccgcgccg gcccccgcgc gc
//
LOCUS       DASWIY010000141        27344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2311955, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000141 DASWIY010000000
VERSION     DASWIY010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 27344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27344
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2311955"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VGA59_05305"
     CDS             complement(<1..444)
                     /locus_tag="VGA59_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719129.1"
                     /translation="MNPGEAKARGVPRTASRIILAALGATFAAWLLWFAASTHLQRAC
                     LVLDTPYLPLCPVAAPESDPARLQDLRTRLRENPGDSGAWVALASQESGANRQALLGA
                     VATLAPSDPEALRLRALDALAKNQMPMAVALLVKMTDHRIGGAEPP"
     gene            complement(588..1172)
                     /gene="metW"
                     /locus_tag="VGA59_05310"
     CDS             complement(588..1172)
                     /gene="metW"
                     /locus_tag="VGA59_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX9719130.1"
                     /translation="MSDRLTMQSIAALVPPGSRVLDLGCGDGAMLDLLQRERNCSGYG
                     IEIADANVLACVKRGVNVIQLNLDEGLSMFDDESFDVVLQIDTLQHLHNTEIMLVETA
                     RVGRIGIVAFPNFAHWPNRLSIARGRMPVTKRLPYQWYDTPNIRVGTFKDFEVLATKN
                     RLAILDAYGLQEGREIRFLPNARASTAVFKIERG"
     gene            complement(1169..2443)
                     /locus_tag="VGA59_05315"
     CDS             complement(1169..2443)
                     /locus_tag="VGA59_05315"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX9719131.1"
                     /translation="MIVTPQSMDFAEPLDLQSGASIRGYSLAYETYGALNADKSNAVL
                     ICHALNASHHVAGTYEGQENSDGWWDTMIGPGKPVDTDRFFVIGVNNLGSCFGSTGPM
                     HVNPDNGKVYGADFPVVTVEDWVNAQARLLDGLGIEKLAAVMGGSLGGMQALSWTLQY
                     PQRVAHAVVVASAPNLTAENIAFNEVARRAIVTDPDFHGGHFYQHGVIPKRGLRIARM
                     IGHITYLSDDVMNEKFGRQLRERIDPAAALAAAQGLLARDAGRSVGDYLYSTQDVEFQ
                     IESYLRYQGDKFSEYFDANTYLLITRALDYFDPARRHNGNLTKALAPATARFLLVSFL
                     TDWRFAPRRSREIVKALLDNRRDVSYAEIDAPHGHDAFLLEDPRYMGVVRSYFERIGK
                     EIGSGAATPPVGAKALPVPGAAILAANAGAPA"
     gene            complement(2565..3128)
                     /locus_tag="VGA59_05320"
     CDS             complement(2565..3128)
                     /locus_tag="VGA59_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX9719132.1"
                     /translation="MEAFLVSTGIVALAEMGDKTQLLSLVLAARFRKPWPIVLGILVA
                     TLANHALAGAAGAWVTTVLGPQVLRWVLGGSFIAMAAWMLIPDKLDEGGGDKAPRLGV
                     FGTTLIAFFLAEMGDKTQVATVMLAAQYAGWLWVVAGTTLGMMLANVPVVWFGDRVTR
                     RVPIRVVHLVSAVIFAGLGAGALAGWG"
     regulatory      complement(3132..3317)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            3439..4641
                     /locus_tag="VGA59_05325"
     CDS             3439..4641
                     /locus_tag="VGA59_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX9719133.1"
                     /translation="MEALKQGSDALFILLGGIMVLAMHAGFAFLELGTVRKKNQVNAL
                     VKILADFSVSTVMYFVVGYGVAYGTNFLVGAEQLAARNGYELVKFFFLLTFAAAIPAI
                     VSGGIAERARFGPQLMATAVLVGFVYPLFEGVAWNQAFGVQAWIKSVSGQEFHDFAGS
                     VVVHAMGGWLALTAVILLGARSNRYRKDGAMSAHPPSNIPFLALGAWILTVGWFGFNV
                     MSAQTIDKISGLVAVNSLMAMAGGTLAALAVGRNDPGFVHNGPLAGLVAVCAGSDLMH
                     PLGALVVGAIAGGMFVAMFTLTQNRWKIDDVLGVWPLHGLCGAWGGIAAGIFGSKALG
                     GLGGVSLGAQVIGTSIGVAWALVAGFLVYGAIKAVTGLRLTPEEEHAGADIAIHRIGS
                     TPEREAAW"
     gene            4761..5327
                     /locus_tag="VGA59_05330"
     CDS             4761..5327
                     /locus_tag="VGA59_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9719134.1"
                     /translation="MTIESQLGEYQGYLLRFARLQLRNDAWAEDAVSETILAALSKPQ
                     SFANKSQLKTWLVGILKHKVIDILRQRQREVSFQDDEGDGSEELDAMMFKADGHYASP
                     PADWGDPEQELNSRQFFAVLEACAEKLPAAMGRIFLMREWLELPSEEICKELKLTPTN
                     LYVQLHRARLRLRECLELNWFGKRAAAS"
     gene            5324..5515
                     /locus_tag="VGA59_05335"
     CDS             5324..5515
                     /locus_tag="VGA59_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX9719135.1"
                     /translation="MILRRTCKEVTALLVAREDRQLPLVERLALRLHLAACRACPVFE
                     RQLSTMRSALQRWRHYSDE"
     gene            5687..6064
                     /locus_tag="VGA59_05340"
                     /pseudo
     CDS             5687..6064
                     /locus_tag="VGA59_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868029.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            6337..6816
                     /locus_tag="VGA59_05345"
     CDS             6337..6816
                     /locus_tag="VGA59_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9719136.1"
                     /translation="MGNKLYVGNLPYSVRDSDLEQAFGQFGAVTSAKVMMERDTGRSK
                     GFGFVEMASDAEAQAAITGMNGQPLGGRSVVVNEARPMESRPPRSGGYGGGGGYGGGG
                     GGSGGGGYGGGGGGRNGGGGYGGRGGGGGSDGGFRSPYGSGPRGGGSGGGGGNRGGY"
     gene            complement(6918..7700)
                     /locus_tag="VGA59_05350"
     CDS             complement(6918..7700)
                     /locus_tag="VGA59_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9719137.1"
                     /translation="MDKLVFITGASSGIGQALAWRYHQAGWRLALAARRGAEIEDWAR
                     AKGLAAGRYAVYRADVADTDSIVAAGRDCLARQGVPDAVIASAGISLGVDSAAREDID
                     VIARTFATNNVGLAATFHPFIEAMARRGSGALVGIASVGGIRGLPGHGAYCASKAAVI
                     AYCESLRGELRASGVKVVTVCPGYVATPLTARNRYGMPFLMQPQDFADRAFRAIEAGA
                     SYRVIPWQMAVVAKLLRVLPNALFDRLLAGRPRKHRQGQVGG"
     gene            complement(7708..8319)
                     /gene="lptC"
                     /locus_tag="VGA59_05355"
     CDS             complement(7708..8319)
                     /gene="lptC"
                     /locus_tag="VGA59_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX9719138.1"
                     /translation="MLLRAAWDRVAVYLPIILMGLMALGTYWLARNTPSLGSVEAQRP
                     PTHDPDYFMRRFSVKTFDAAGRLKSQVHGTEARHYPDTDTLEIDQPRLRSFNPRGELT
                     LATARRALSNADGSEVQLFGDAVITREATRGARGEARPRMEFRGEFLHVFTDTERVKS
                     HKPVALTRGNDRFTADSMDYDNLDRVMELRGRVRGVLTPDSGS"
     gene            complement(8382..8927)
                     /locus_tag="VGA59_05360"
     CDS             complement(8382..8927)
                     /locus_tag="VGA59_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9719139.1"
                     /translation="MERPALSFPPEQLLAAQGVRVAFFDVDGVLTDGGLYQSDQGESL
                     KRFHILDGLGLKLLQRAGITPAVITGRDSKPLRARLQALGVAHVHYGSEDKRPAAEKT
                     LQELGLQWKQAAAIGDDWPDLPLLRRCAFAAAPANAHAEVKAAAHYVTRAAGGHGAAR
                     EFCDLLLQASGRYAQLLEGYA"
     gene            complement(8950..9948)
                     /locus_tag="VGA59_05365"
     CDS             complement(8950..9948)
                     /locus_tag="VGA59_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX9719140.1"
                     /translation="MALRQVQDKAFDTTQALRLAQETFDIEAAAVLGLKARTNEAFGR
                     AVELALGVRGRVVVMGMGKSGHIGRKIAATLASTGTPAMFVHPAEASHGDLGMIKPVD
                     MVLAISNGGESEEITVILPVLKRLGVPLVAMTGGLDSTLARHADIVLDCGVEKEACPL
                     NLAPTASTTAQLALGDALAVALLDARGFRTEDFARSHPGGALGRKLLTHLSDVMRTGD
                     AVPRVGPDTSFSDLMREMSAKGLGASAVVDKGHVLGIFTDGDLRRLVEKGLDLRGLTA
                     REVMHPNPRTIRYDVLAVEAAELMEQQRITSVLVVDDEGKLCGAVNSNDLMRAKVI"
     gene            9997..11982
                     /locus_tag="VGA59_05370"
     CDS             9997..11982
                     /locus_tag="VGA59_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9719141.1"
                     /translation="MTSLELTLLYLLAAVIGVVACRLVKLPPMLGYLVVGVLIGPNAM
                     ALGEDIPAVRHLAEFGVVFLMFVIGLEFNLHKLRAMRAHVFGLGLFQVVFTIALATAG
                     SLLIASVVPRLWLMNWQTALVLAGVLAMSSTAIVVKLLADRLELESEHGKRVIGVLLF
                     QDLAVVPLLVLIPALSAPPDMLFKALALASLKAAALIALLLAGGQRVMRWWLTLVARR
                     KSEELFVLNLLLVTLGLAWLTELAGLSLALGAFIAGMLISETEYKHQVETDIRPFHDV
                     LLGLFFITIGMLLDWRIVAQHWWLVLLLVTLPVLFKLLLVAGLTRALGASAGVSLRTG
                     LYLAQAGEFGFVLLTLANQQELVPPVLLNPILASMVLSMLATPFIIMYSNRIVMKLVS
                     SEWMLQSLQMTNIARKSINTNRHVIICGYGRCGQNLARMLEHEGIPYIALDLDPDRVR
                     QATAAGDSVVFGDASRLQALVAAGLARASAVVVTYLDVPGALKVLSRAHDHAPQVPVI
                     VRTQDDRDLEELQAAGATEVVPELLEGSLMLASHALALVGVPMRRVIRIVQDQRDARY
                     NLLRGYFHGADDDTVSELENERLSTVTLPFGAGSLGHALSQMALQALDVRVISLRRSN
                     GKPVDPDTDPPLEDGDTLVLSGRPETLAIAEEKLLHG"
     gene            complement(12016..12465)
                     /locus_tag="VGA59_05375"
     CDS             complement(12016..12465)
                     /locus_tag="VGA59_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9719142.1"
                     /translation="MNVEQLVRAIAALDATDALQCKLTLDEWKALAPYLSMRFLKPGD
                     PLMHEGDCERELFFLADGELEVTILGTPVATLKAGSVVGEGTFFSGAPRSATVTPSQP
                     GVAWGMNRDKFDAMASKHPRLALDLVKGLAAVLAVRMREALLVGQFT"
     gene            complement(12564..13394)
                     /locus_tag="VGA59_05380"
     CDS             complement(12564..13394)
                     /locus_tag="VGA59_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719143.1"
                     /translation="MSLFSWLIPAFRPAKVQAESSGLSRIEPTQPVMKRQAGSEPVAA
                     NGQPANRKSERMARRELLYAVVRESMMRAGVLSASYKFKVLSLDARGRQFLVMVDLAR
                     DNGGETARLAEIEALIAQAAKSRHDILVTAVYWRSNEHVAIGDPGVALQRGTDSRPVP
                     LDPEPDALASQPTPLDSGPVPLAPTAPPRTLSRYEPIQADEVAAFKRALAAGVSNPAA
                     AAAAAVGVSHGASAKSFDGSAMHGPQSYTLLTGFEDTEMPDTDQRMPSLSGTQYGDLN
                     "
     gene            complement(13579..14058)
                     /locus_tag="VGA59_05385"
     CDS             complement(13579..14058)
                     /locus_tag="VGA59_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9719144.1"
                     /translation="MRAPLRFDVKGLVKAIAESPSSDSFSPALTATQWELLGTYMQPF
                     ALMQGQVLIEQNAQDRNVYLVESGSLTVHYEDDKGRLRLALVGPGSAVGEGAFFTRLP
                     RSATVQSASPGKVWCLTPIRFTELTNRQPAMALEIAMALGSLVSRRLANKPKRVAVT"
     gene            complement(14185..14937)
                     /locus_tag="VGA59_05390"
     CDS             complement(14185..14937)
                     /locus_tag="VGA59_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9719145.1"
                     /translation="MSATLLQLRDFCVSYGAVQAVHGVELSVREGEIVTVIGPNGAGK
                     STLLCAVMGLLPSSGTLALDEHTIARPTVEAMVARGVALVPERRELFGEMSVEDNLLL
                     GGFWRWRKGQRDQARRMQEVFALFPRLEERRAQEASTLSGGERQMLAIGRALMAEPRL
                     LMLDEPSLGLAPLIVREVLEMVASLRKLGVSVLLVEQNARAALQVADRAYVLEMGAVA
                     LSGTAQALLHDRRIIDTYLGIGKKADPLVAVT"
     gene            complement(14934..16760)
                     /locus_tag="VGA59_05395"
     CDS             complement(14934..16760)
                     /locus_tag="VGA59_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX9719146.1"
                     /translation="MTRRGLTLLAVLALAVAWPWLPEFTITLLCYIGLYSMVAAGLVM
                     LTGVGGMTSFGQAAFVGVGAYATAWVTTSPAFAAAVGGLIAPIWLPWLGLLLGLVVAA
                     AVAWGLGAITLKLSGHYLPLCTIAWGLSIYFLFGNLEVLGGQTGTGGLPPLMVGSLSL
                     AAPRALGPVIWATLLLALWALHNLLDSREGRAIRALKGGRLMAESMGVDTAAYRVKIF
                     VLAALLAALSGWFYAHLQRFVNPTPFNLNIGIEYLFMAVVGGAGHLWGAVLGAALVTL
                     LKSRLQDVLPQLLGSSGNFEVIAFGLLMLVVLHRFADGLWPTLAAVSHRFLRPDSTAV
                     RPDSEPLPELQLPPAGQVLLRTSQASKRFGGLTAVNSVSLDVVAGEVHALIGPNGAGK
                     TTFFNMVSGVIDPSSGEVSLMGKSMTGHPAREHAALGLGRTFQHVRLLGQRSVLDNVA
                     LGAHRRGRRGWLASMLRLDRAEEAALLAQARRQIERCQLGPHVNAPAGSLSLGKQRAI
                     EIARALAGHPALLLLDEPAAGLRHLEKVALAQLLAQLRAEGLGILIVEHDMEFVMNLA
                     DRVTVLEFGTVIAHGTPAQVQADPRVLDAYLGGADEPLEVAA"
     gene            complement(16760..17785)
                     /locus_tag="VGA59_05400"
     CDS             complement(16760..17785)
                     /locus_tag="VGA59_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9719147.1"
                     /translation="MDLQIALLLLQDGIVNGAIYGLMGLALVLVFSVTRIIFIPQGEL
                     VAFAALSMAALNSGSAPRTLWLLLGLAVATLLVEAWRQRRGAAVDWASSLVWAVLFPA
                     LAGALVLLRPSSLALQALTTLVVVTPLGPLLYRLAYRPLADASVLMLLIVSVALHGVL
                     VGLGLLFFGAEGSRTPAFSDLRLDLGGLAVTGQSLVVVGVTLVLVIAMFRFFGRTMVG
                     KALRATAINRVGARLMGIPTELSGDLSFALAGLIGAVSGLLIGPLTTVYYDTGFLIGL
                     KGFVAAIVGGMASYPLALGGALLVGQLESFSSFWASAYKEVLVFTLIIPVLWWRSLHS
                     HHVEEDE"
     gene            complement(17905..19050)
                     /locus_tag="VGA59_05405"
     CDS             complement(17905..19050)
                     /locus_tag="VGA59_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9719148.1"
                     /translation="MKLLKPVALAAALLASTLALADINVGVTLSATGPAASLGIPEKN
                     TIALMPKTIAGQKINYIVLDDASDTTAAVTNTRKLITENKVDVILGSTITPNSLAMID
                     VVAEAKTPMISMAASAKIVEPVDAKKAWVFKTPQNDIMMSQAIVEHMAANGVKTVAFI
                     GFSDAYGEGWNQEFTKAAAAKNLKIVANERYARTDTSVTGQALKLIAAKPDAVLIAGS
                     GTPAALPQKTLKERGFAGKIYQTHGIANADFLRVGGKDVEGTILPAGPVLVADQLPDS
                     HPVKKSAMAYIAAYEGAHGKGSVSTFGGHAWDAGMLMAAAIPGALKKAQPGTAEFRAA
                     LRDSIEQVKGVAGAHGIFTMSKTDHLGLDKNARVMVKIENGTWKYTP"
     gene            complement(19199..20620)
                     /gene="mnmE"
                     /locus_tag="VGA59_05410"
     CDS             complement(19199..20620)
                     /gene="mnmE"
                     /locus_tag="VGA59_05410"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX9719149.1"
                     /translation="MLARHTQPIAAIATAPGRGAVGIVRVSGPSLAALIEAVCGRALK
                     PREATYLPFLAADGSIIDQGLAIHFPAPHSYTGEDVLELQAHGGPVVLQLLLARCLEA
                     GGEADPATGKPRLEGLRVAEPGEFTQRAFLNDKIDLAQAEAIADLIDASTEAAARSAS
                     RSLSGEFSREIHALRDALIHLRMLVEATLDFPDEEIDFLQKADAQGQLERLRQTLARV
                     LQRARQGALLREGIKVVIAGQPNAGKSSLLNALAGAELAIVTAVPGTTRDVVSGAIQI
                     EGVPLHVIDTAGLRDSQDHVEKIGIERAWGQIENADAVLFLHDLTRARDADYAVGDEA
                     IARGLSDKLPRTVPVIEVWNKLDLVAPGVAAAASAADSVLLSAKTGAGLDALRRRLLE
                     AAGWQAAPEGIYIARERHVQALRRVEAHLLEAAGHLAAQAQALDLLAEELRLAQNALN
                     EITGEFTPDDLLGVIFSSFCIGK"
     gene            complement(20648..20962)
                     /locus_tag="VGA59_05415"
     CDS             complement(20648..20962)
                     /locus_tag="VGA59_05415"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEX9719150.1"
                     /translation="MTIALNPLAAFPLSSWFRAVRLPRRAAAHRVVPALQGPMSIDKG
                     ATLVVERALGANVTCVSGSLWITHDGGIKDILIEAGDTYLSDRSSRMLVHGLAASTAL
                     VR"
     gene            complement(21090..22157)
                     /locus_tag="VGA59_05420"
     CDS             complement(21090..22157)
                     /locus_tag="VGA59_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9719151.1"
                     /translation="MSIHQDIRFCSTADGVQLAMALYGDGPPLVKAATWLTHIEADPT
                     SVLNPHWIEELSRRHRYVTYDARGCGLSSRNVADISFEAWVRDLETVVDSLGLERFPL
                     LGISQGAAIAVAYAARHPDRVSAMVLWGGFATSYFTTGNPDPKIVDEGETLLKLVELG
                     WGKGSPAFRQVFVSKLMPDSTAAEQHEFDQYQNLTATPDVAVRCLRAMYLVNVKQEAR
                     KVQCPAIVFHARDDQMIYFDQGRKLAGLIPGARLVPVNSRNHLPLAREPCWPEVVLEL
                     RAFLGEPVKARAEGTRLTARQHEVLKQVAAGLTDKQIARALSLSPRTVEMHVASAMKA
                     LDCATRAEAVHRAAQAGLLAP"
     gene            complement(22400..24094)
                     /gene="yidC"
                     /locus_tag="VGA59_05425"
     CDS             complement(22400..24094)
                     /gene="yidC"
                     /locus_tag="VGA59_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX9719152.1"
                     /translation="MNDIRRTILWVIFGFSMVLLWDQWQIYNGSKPTFFPSTPIPPAA
                     TAPGAPAPSGVPPASTVPAPTATAPAAVPGAVPPTPAMTSAVGEKFTVTTDLLHLTFD
                     TQGGSLVRTEFLKQAGENKTGTFVLLDERPGYKYVAETGLIGGDFPNHKTVMTPSAER
                     ELKAGANELVVRFESPEKGGVKLVKSYTFKRGAYDAVVRHEVLNTGTAPVSPQLYLQL
                     VRDGNKVPGESSFYFTFTGPAVYTEAKKFSKVEFSDIEKGKVDIDKQSSDGYVAMVQH
                     YFASAWILPDGVKRELFARKVDTNLYAVGMIAPIEPIAPGLARAVDAKLFAGPQVEKQ
                     LEQIATGLDLVKDYGWLTILSKPLYWLMDKIYDVLGNWGWSIMALVLIIKIAFYWLQA
                     KGYESMAKMKAINPKVMAMRERFKDNPQQMQQEMMRIYREEKVNPMGGCFPIMVQIPV
                     FIALYWVLLSSVEMRGAPWIGWIRDLSTPDPYFILPVVMTLTTLLQTALNPTPPDPMQ
                     AKLMWFMPLAFSIMFFFFPSGLVLYWITNNVLSIAQQWLINTRMGVPPQFNLPKFR"
     gene            complement(24091..24372)
                     /gene="yidD"
                     /locus_tag="VGA59_05430"
     CDS             complement(24091..24372)
                     /gene="yidD"
                     /locus_tag="VGA59_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX9719153.1"
                     /translation="MIRALLIALVKGYRLLLSPWLGSSCRFTPTCSAYSLQALERHGA
                     AAGTYLTLARLARCHPWCEGGHDEVPEQAPTLTRLFTHLLPSLSKKKSS"
     gene            complement(24369..24827)
                     /locus_tag="VGA59_05435"
     CDS             complement(24369..24827)
                     /locus_tag="VGA59_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX9719154.1"
                     /translation="MQRLKTRAQFEAVMAGTTVSRTAHFALHRRPLDAPPAPDAGPGS
                     AGPASAGRFSNAPMGPGSAGPKALFAFRDSWIGALVPKRWAKRAVTRNAIKRQIYTVS
                     QELQPPLPAAAHVVRLRAGFDRTQFISATSGALKRAVRAELQQLFAQAAQ"
     gene            complement(24867..25001)
                     /gene="rpmH"
                     /locus_tag="VGA59_05440"
     CDS             complement(24867..25001)
                     /gene="rpmH"
                     /locus_tag="VGA59_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX9719155.1"
                     /translation="MKRTYQPSKVRRARTHGFLVRMKTRGGRAVINARRAKGRKRLAV
                     "
     gene            25459..26871
                     /gene="dnaA"
                     /locus_tag="VGA59_05445"
     CDS             25459..26871
                     /gene="dnaA"
                     /locus_tag="VGA59_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX9719156.1"
                     /translation="MSEGQHNSLAAHAGIGAGDSLWQACIDQLAQELPEQQFNTWIKP
                     LVAQVSDDFSRVTLFVANRFKLDWVRAQYSSRIAGTLEKLYGQPVHLELALALRESPS
                     RTYSSPASSEMGAAPEPAEISDDGAAAAFKNRLNTALTFDTLVEGTANRMARAAAMHV
                     STTPGHLYNPLFIYGGVGLGKTHLVHAVGNRLLADKPGSKVLYIHAEQFVSDVVKAYQ
                     RKTFDEFKERYHSLDLLLIDDVQFFANKDRTQEEFFNAFEALLAKKSHIVMTSDTYPK
                     GLADIHERLISRFDSGLTVAIEPPELEMRVAILINKARAESAEMPEEVAFFVAKNVRS
                     NVRELEGALRKILAYSRFNQKEISIMLAREALRDLLSIQNRQISVENIQKTVADYYKI
                     KVADMYSKKRPASIARPRQIAMYLAKELTQKSLPEIGELFGGRDHTTVLHAVRKIGGE
                     RQQMTELNQQLHVLEQTLKG"
BASE COUNT         4525 a         9040 c         9233 g         4546 t
ORIGIN      
        1 cggtggctcg gcgcccccga tgcggtggtc ggtcatcttc accagcagcg caacggccat
       61 aggcatctgg ttcttggcca acgcgtccag cgcgcgcaac cgaagcgcct ccggatcgct
      121 gggcgccagt gttgcgacgg cgcccagcag cgcctggcgg tttgcgcccg attcctggct
      181 tgccagcgcc acccaggcgc cggagtcgcc cgggttttcg cgcaggcgcg tgcgcaggtc
      241 ctgcaggcgg gccgggtcgg actcgggcgc tgccacgggg cacagcggca agtaaggcgt
      301 gtccagcacc aggcacgccc gctgcaagtg agtggacgct gcaaaccaca gcagccaggc
      361 ggcgaatgtc gcgcccagtg cggccagaat gatccgggat gccgtgcggg gaacgccccg
      421 tgcctttgcc tcgcctggat tcatttcagt tgctccaccg cgctgcgcgt gacccgcgcc
      481 gcatgaaacg gttgtcattg cgggcttgac ccgcaatcca tgggagcctg ctgccacgcg
      541 acgatggatc ccgggtcaag cccgggatga caaattcgag tttcatgtca gccgcgctcg
      601 atcttgaaga cggccgtgct ggcccgcgca ttgggcagga accggatctc gcgcccttcc
      661 tgcagcccgt aggcgtccag gatcgccagc cggttcttgg tcgccagtac ctcgaagtcc
      721 ttgaaggttc cgacccggat gttgggcgtg tcataccact ggtagggcag gcgctttgtc
      781 accggcatgc gcccgcgcgc gatggaaagc cggttgggcc agtgggcgaa gttcgggaac
      841 gcgacgatgc cgatgcggcc cacgcgtgcc gtctcgacca gcatgatctc ggtgttgtgc
      901 aggtgctgca gggtgtcgat ctggagcacc acatcgaacg actcgtcgtc gaacatcgac
      961 aaaccttcgt cgaggttgag ctgaatgacg ttgacgccgc gcttgacgca ggccagcacg
     1021 ttggcgtcgg cgatctcgat gccgtagccg ctgcaattgc gctcgcgctg cagcaagtcg
     1081 agcattgcgc cgtcgccgca tcccagatcc agcacgcgcg agcctggcgg caccagggcc
     1141 gcgatcgatt gcatggtcag gcgatcgctc atgccggtgc ccccgcgttt gcggcaagga
     1201 tcgctgcacc gggcactggg agggccttcg cgcccacggg cggcgtcgcg gcgccgcttc
     1261 cgatttcctt gccgatgcgc tcgaaatagg agcgcaccac gcccatgtag cgcgggtctt
     1321 ccagcaggaa ggcgtcgtgg ccgtgcggcg cgtcgatctc ggcatagctc acgtcgcgcc
     1381 ggttgtccag cagggccttg acgatctcgc ggctgcgccg cggggcgaag cgccagtcgg
     1441 tcaggaagct caccagcagg aagcgcgcgg tggccggcgc cagggccttg gtcaggttgc
     1501 cgttgtggcg gcgcgccgga tcgaagtaat cgagggcccg cgtgatcagc aggtaggtgt
     1561 tggcgtcgaa gtattcgctg aacttgtcgc cctggtagcg caggtagctc tcgatctgga
     1621 attcgacgtc ctgtgtggag taaaggtagt cgccgacgct gcgcccggcg tcccgcgcga
     1681 gcaggccttg cgccgccgca agggccgccg cggggtcgat gcgttcgcgc agctgccgcc
     1741 cgaacttctc gttcatcacg tcgtcgctca ggtaggtgat gtgcccgatc atgcgcgcga
     1801 tgcgcaggcc gcgcttgggg atcacgccgt gctggtagaa atggccgccg tggaaatcgg
     1861 ggtcggtcac gatggcgcgc cgcgccacct cgttgaaggc gatgttctcg gccgtgagat
     1921 tgggggcact ggcgacgacc acggcatgcg ccacgcgctg cggatattgc agcgtccacg
     1981 acagcgcctg catgccgccc aggctgccgc ccatgacggc ggccagcttc tcgatgccca
     2041 ggccgtcgag caggcgggcc tgggcgttga cccagtcttc caccgtgacc accggaaaat
     2101 ccgcgccata gaccttgccg ttatccggat tcacgtgcat cggcccggtc gagccaaagc
     2161 acgaacccag gttgttgacg ccgatgacga agaagcggtc ggtgtccacc ggcttgccgg
     2221 ggccgatcat cgtgtcccac cagccgtccg aattttcctg gccttcgtag gtgcccgcca
     2281 cgtggtgcga cgcgttgagc gcatggcaga tcaggacggc attgctcttg tccgcgttca
     2341 gcgcgccgta ggtctcgtag gccagtgaat agccgcgtat cgacgcgccg ctctgcaggt
     2401 cgagcggttc ggcgaagtcc atcgattgcg gcgtgacgat catgaaaaca ggaaacccgg
     2461 cgacgctaaa gaggcgaggc cgggtccgtc agggctttcg gtctttagct gaatttattg
     2521 agcgcccgca agctgaagca aatcggcgct gtgcctgcag tatatcaacc ccatccggcc
     2581 agcgcgccgg cccccagccc ggcgaagatg acggccgaca ccaggtgcac gacgcggatc
     2641 ggcacccgcc gcgtcacccg gtcgccgaac cagactaccg gcacattggc cagcatcatg
     2701 cccagcgtgg tgccggccac cacccacagc cagccggcgt actgcgccgc cagcatcacc
     2761 gtcgccacct gcgtcttgtc gcccatctcg gcgagaaaga aggcaatcag cgtggtgccg
     2821 aaaacgccca ggcgcggagc cttgtcgccg ccgccctcat cgagcttgtc gggaatgagc
     2881 atccacgccg ccatcgcgat gaacgatccg cccagcaccc agcgcagcac ctgcggcccg
     2941 agcaccgtgg taacccaggc cccggcggcg ccggcaaggg cgtggttggc cagggtggcg
     3001 acgaggatgc ccagcacgat cggccagggc ttgcgaaagc gggcggccag caccagcgac
     3061 agcagctggg ttttgtcgcc catttcagcc agggcaacga tgccggtcga gacgaggaag
     3121 gcttccatga caggggtccc aggccggggc ggagcacaca ttgactgcgc ggcgtccccg
     3181 gccaaagcgg aggctgcgca gtcaaaggtc tcgccaggtc cggaactacc gtttgcgcca
     3241 tggggcgcaa ggccccaagt gtgttgacgc aaaccccttt cggaatcgaa gggaggctac
     3301 tccccaatga cggcatgcat tgtagccgcg acaagccggc gcacgcttct ggcgcactta
     3361 ttgcttacat atggtgcgaa taaccgtttg cgcggcggag cgcaccaata acaggcaaat
     3421 ttatccaaag agggtttcat ggaagcacta aaacagggct cggacgcttt gttcatcctg
     3481 ctgggcggca tcatggtgct tgccatgcac gccggtttcg catttctgga gctggggacc
     3541 gtgcgcaaga agaaccaggt caacgccctg gtcaagatcc tggccgattt ctcggtttcc
     3601 acggtgatgt acttcgtcgt gggctatggc gtggcctacg gcacgaactt cctcgtcggc
     3661 gccgaacagc tggcggccag gaacggctac gagctggtga agttcttctt cctgctgact
     3721 ttcgccgcag ccatcccggc catcgtctcc ggcggcattg ccgagcgggc gcgcttcggt
     3781 ccccagctca tggccacggc ggtcctggtc ggcttcgtct atccgctgtt cgagggcgtg
     3841 gcctggaacc aggctttcgg cgtccaggcc tggatcaagt cggtatcggg ccaggagttc
     3901 catgacttcg ccggctcggt ggtggttcac gccatgggcg gctggctggc gctgaccgcc
     3961 gtgatcctgc tgggcgcgcg cagcaaccgc taccgcaagg acggggcaat gtcggcccac
     4021 ccgccctcca acattccgtt cctggcgctg ggagcgtgga tcctgaccgt cggctggttc
     4081 ggcttcaacg tcatgagcgc ccagaccatc gacaagatct ccggcctggt ggcggtcaat
     4141 tcgctgatgg cgatggccgg cggcacgctc gcggccctgg cggtcggccg caacgacccc
     4201 ggcttcgtgc acaacggtcc gctcgcggga ctggtcgcgg tgtgcgcggg ttccgacctg
     4261 atgcatccgc tcggtgcgct cgtggtcggc gcgatcgcgg gcgggatgtt cgtggccatg
     4321 ttcaccttga cgcagaacag gtggaagatc gacgacgtgc tgggcgtctg gcccctgcac
     4381 ggcttgtgcg gcgcctgggg cggcattgcg gcgggcatct tcggcagcaa ggcgctgggc
     4441 gggttgggcg gcgtcagctt gggcgcccag gtcatcggca cctcgatcgg cgtggcgtgg
     4501 gcgctcgtcg cgggtttcct tgtctacggg gcgatcaagg ccgtgacggg cttgcgcctg
     4561 acgccggagg aagaacacgc gggcgcggac atcgccatcc accgcatcgg ctcgactccc
     4621 gagcgcgaag ccgcctggta atcaagccgg ggccgccctc tccagcgtgc cgggaaaaag
     4681 ccgcggggca gctcgccggg tttaccctga gcctctcgcc cgctggacgg accgatcctt
     4741 cgttcctaca gttcacaccc atgacgatcg aaagccaatt gggcgagtac cagggctacc
     4801 tgctgcgatt cgcgcgcctg cagctgcgca acgacgcctg ggccgaggac gcggtctcgg
     4861 aaacgattct ggccgccctg tccaaaccgc agtcgttcgc caacaagagc cagctcaaga
     4921 cctggctggt gggcatcctc aagcacaagg tgatcgacat cctgcgccag cggcagcgcg
     4981 aagtgagctt tcaggacgac gagggcgacg gcagcgaaga gctcgatgcc atgatgttca
     5041 aggccgacgg gcactacgcc tcgcctccgg cggactgggg cgaccctgag caggagctca
     5101 attcgcgcca gttcttcgct gtgctggagg cttgcgccga aaagctgccg gcggccatgg
     5161 gccggatttt cctgatgcgc gagtggctgg aactgccgag cgaggaaatc tgtaaggaac
     5221 tgaagttgac gccgaccaac ttgtacgtgc agctgcaccg cgcgcggctg cggctgcgcg
     5281 aatgcctgga actcaactgg tttggaaagc gcgccgccgc ctcatgatct tgcgccgcac
     5341 ctgcaaagaa gtgaccgccc tgctggtggc aagggaagac cgccagctgc ccctggtcga
     5401 gcggctggcc ttgcgcctcc atctggccgc gtgcagggcc tgcccggtgt tcgagcggca
     5461 gctgtcgacc atgcgcagcg ccctgcagcg ctggcgccat tactcggacg aataaaaagg
     5521 gcttggaaac gccgggctgt acacaaacgc cccggatgcc ccataatcca gggggtgtca
     5581 cgtccgcgtg gcacaagatt gcggtgaatg gtcggtttcg cgtggtttgt ttattgcgtt
     5641 tttcggactc cactgcttag tgcgtttttt ttccaggagt ccctgcatgg gcaacaaact
     5701 ttacgtgggc aacctgccct attcttaccg cgacagtgac atggagcagg cgttcagcca
     5761 atacggcacc gtttccagcg ccaaggtcat gatggagcgc gacaccggcc gctccaaggg
     5821 tttcggcttt gtcgagatga gcaccgaggc ggaagccaag gctgccatcg aaggcatgaa
     5881 cggccagcag atcggcggcc gtggcctggt ggtcaacgaa gcgcgtccga tggagtcgcg
     5941 gcccccgcgt agcggcggct tcggcggcgg tggtggcggc ggctacggcg gtggcgctgg
     6001 cggcggcggc ggcggtggcc gtcgtgaagg cggcggtggc ggctacggcg gtggccgcgg
     6061 cggctactga tcagctgtcc gttactcggc aaattcatca aaagggccta cgggcccttt
     6121 tttcatgcgc gggaatcaag ggggctgccg ccgcgcacag ccgccccggg cttgcacaca
     6181 ctccagtcat atgttccata attggaaaag cgtggttagc ggttttcacg caggattgcg
     6241 gtgaacaggt cacttttcgc gtcgctcagg ctcagtggta tgcgttatcg ggactccatc
     6301 gctcaaggtc acatgttttt ttaggaggcc ctatcgatgg gcaacaaact ctacgtcggc
     6361 aatctccctt actcggtccg cgacagcgat ctcgaacagg cgttcgggca gttcggcgcc
     6421 gtcaccagcg ccaaggtcat gatggagcgc gacaccggcc gctccaaggg cttcggcttc
     6481 gttgaaatgg cgagcgatgc ggaagcccag gccgccatca ccggcatgaa cgggcagccg
     6541 ttgggtgggc gcagcgtggt cgtcaacgag gcccgtccca tggaatcgcg gcctccgcgc
     6601 agcggtggct atggcggtgg aggtggctac ggcgggggcg gaggcggtag tggcggtggc
     6661 ggctatgggg gtggcggcgg cggccgcaat ggcggtggcg gctacggcgg gcggggcggc
     6721 ggtggcggta gcgacggcgg cttccgcagc ccctacgggt ccggcccgcg tggcggcggc
     6781 agcggtggcg gcggtggaaa ccgcggcggc tactgagtca gggttgagcg ctcggcgttg
     6841 ggcgttgagc gcgcgatgtc accgcattca agggctcccc agggagccct tttgctttca
     6901 cgctcaacgc ggaacactca acccccgacc tgcccctggc ggtgcttgcg gggccggccg
     6961 gcgagcagcc ggtcgaacag cgcattcggc agcacccgca gcagcttggc cacaacggcc
     7021 atctgccaag gtatgacacg gtagctggcg cccgcttcaa tggcccggaa tgcccgatcg
     7081 gcgaaatcct gcggctgcat gagaaacggc atgccatagc ggttgcgtgc cgtcagcggg
     7141 gtcgccacgt agccggggca gaccgtgacc accttgacgc cgctcgcccg cagctcgccg
     7201 cgcaggctct cgcagtaggc gatcacggcg gccttgctcg cgcaatacgc cccatgtccc
     7261 ggcaatccgc ggatgcctcc gacactggcg atgccgacca gcgcacccga gccgcgccgg
     7321 gccatcgcct cgatgaaggg atggaaagtc gccgccaggc cgacgttgtt ggtggcgaag
     7381 gtgcgcgcga tcacgtcgat gtcttcgcgc gccgcggaat ccacgccgag gctgatcccc
     7441 gcgctggcga tcaccgcatc gggcacgccc tggcgcgcca ggcagtcgcg ccccgcggcc
     7501 acgatgctgt cggtatcggc cacgtcggcg cggtacaccg cgtagcggcc ggccgccagg
     7561 cccttggcgc gcgcccagtc ctcgatctcg gctccgcgcc gcgccgccag cgccagccgc
     7621 cagccggcct ggtggtagcg ccaggccagc gcctggccga tcccgctgga agcgccggtg
     7681 atgaaaacca gtttgtccat ggcccgccta ggacccggaa tcgggcgtca gcacgccgcg
     7741 cacgcggccg cgcagctcca tcacccggtc caggttgtca tagtccatgc tgtccgcggt
     7801 gaagcggtcg ttgccacgcg tcagcgccac cggcttgtgc gatttcacgc gctcggtgtc
     7861 cgtgaagacg tgcaggaatt cgccccggaa ttccatccgc ggccgggcct cgccgcgcgc
     7921 gccgcgggtg gcctcgcggg tgatcaccgc gtcgccgaac agctgcacct cggagccgtc
     7981 ggcgttgctc agcgcgcggc gcgcggtggc cagcgtgagc tcgccgcggg ggttgaagga
     8041 tcgcaggcgg ggctggtcga tctccagcgt atcggtgtcg gggtagtggc gcgcctcggt
     8101 gccgtggacc tggcttttca ggcgcccggc ggcgtcgaat gtcttgaccg aaaagcggcg
     8161 catgaaatag tccgggtcgt gggtgggggg ccgctgcgcc tcgaccgatc ccaggctcgg
     8221 cgtgttgcgc gccagccagt aggttcccag cgccatcaat cccatcagga tgatgggcag
     8281 gtagacggcc acccggtccc aggccgcgcg cagcagcatc cgcgcggtcg cccgcggcga
     8341 gcgggcggcg acggcgccca tcggctcccc cctgggcgcg gtcatgcgta gccctcgagc
     8401 agctgcgcgt agcgtcctga ggcctgcagc agcagatcgc agaattcgcg cgcggcgcca
     8461 tggccgccgg cggcgcgcgt cacgtaatgg gccgcggctt tcacttcggc atgggcattg
     8521 gccggcgcag ccgcgaaagc gcagcgtcgc agcagcggca gatccggcca gtcgtcgccg
     8581 atggcggcgg cctgcttcca ttgcaggccg agttcctgca aggttttctc ggcggcgggt
     8641 cgcttgtcct cgctgccata gtgcacgtgg gcgacgccca gcgcctgcag gcgagcgcgc
     8701 aggggtttgg aatcgcggcc ggtgatcacg gcgggggtga tgccggccct ttgcagcagc
     8761 ttcaggccca gcccgtccag gatgtggaag cgcttgaggg actcgccctg gtcggactgg
     8821 tacaggccgc cgtcggtcag cacgccgtcc acgtcgaaga aggcgacacg cacgccttgg
     8881 gccgcgagca gctgctccgg cgggaaactc agggcgggtc gctccatggc gggtcggtgg
     8941 ggatgcgccc tagatcacct tggcgcgcat caggtcgttg ctgttgacgg cgccgcacag
     9001 cttgccttca tcgtcgacga ccaggacgct ggtgatgcgc tgctgctcca tgagttcggc
     9061 cgcctccacc gccagcacgt cgtagcggat ggtgcgcggg ttgggatgca tgacctcgcg
     9121 tgcggtcagg ccgcgcaggt ccaggccttt ttccaccaac cggcgcagat cgccgtccgt
     9181 gaagatcccc agcacatggc ctttgtcgac aacggccgac gcgcccaggc ccttggcgct
     9241 catctcgcgc atcaggtcgc tgaaagaagt gtcgggaccg acgcgcggca cggcgtcgcc
     9301 cgttcgcatc acgtcgctca ggtgggtcag cagcttgcgg ccgagcgcgc cgccgggatg
     9361 ggagcgcgcg aagtcctcgg tccgaaagcc cctggcgtcg agcagggcca ccgccagcgc
     9421 gtcgcccagc gcaagctggg ccgtggtgct ggcggtgggc gcgaggttga gcgggcacgc
     9481 ttccttctcg acgccgcaat cgagcacgat gtcggcgtgc cgcgccagcg tcgagtccag
     9541 cccgcccgtc atcgccacca gcggcacgcc caggcgcttg agcaccggca ggatcacggt
     9601 aatttcctcg ctttcgccgc cgttggaaat cgccagcacc atgtccaccg gcttgatcat
     9661 gccgaggtcg ccgtggctgg cctcggccgg atgcacgaac atcgccggcg tgccggtcga
     9721 ggccagggtg gcggcgatct tgcggccgat gtggccgctc ttgcccattc ccatcacgac
     9781 cacccgcccg cgcacgccca gcgccagttc cacggcgcgc ccgaaagcct cgttggtgcg
     9841 cgccttcagg cccagcaccg cggccgcctc gatatcgaag gtttcctggg ccaggcgcag
     9901 cgcctgcgtg gtgtcgaacg ccttgtcctg aacttgccgg agtgccatgg cgcgattcta
     9961 tagagccgtg acgaaattcc cttggtagca tgggggatga cctccctcga actcacgctg
    10021 ctgtacctgc ttgccgcggt gatcggcgtg gtggcctgcc ggctggtcaa gctgcccccc
    10081 atgctgggct atctggtggt cggcgtgctg atcggcccga acgcgatggc gctgggagag
    10141 gacatcccgg cggtgcgcca cctggccgaa ttcggcgtgg tcttcctgat gttcgtgatc
    10201 ggattggaat tcaacctgca caagctgcgc gccatgcgcg cccatgtatt cggcctgggc
    10261 ctgttccagg tggtgttcac gatcgcactt gccaccgccg gctcactgct gatcgcctcc
    10321 gtcgtgccca ggctctggct catgaactgg caaacggcgc tggtgctggc gggcgtgctg
    10381 gcgatgagca gcacggccat cgtcgtcaag ctcctggccg atcggctgga gctggaaagc
    10441 gagcacggca agcgggtcat cggcgtgctg ctgttccagg atctggccgt ggtgcccctg
    10501 ctggtgctga tccccgccct gagcgctccg ccggacatgc tgttcaaggc gctggcgctg
    10561 gcgagcctga aagcggcggc gctgatcgcg ctgctgctgg ccggcggcca gcgcgtgatg
    10621 cgctggtggc tcacgctggt ggcacggcgc aagagcgaag agctgttcgt gctgaacctg
    10681 ctgctggtca ccctcgggct ggcctggctt accgagctgg cggggctgtc gctcgcgctc
    10741 ggggccttca tcgccggcat gctgatttcc gagaccgaat acaagcacca ggtggaaacc
    10801 gacatccggc cgttccacga cgtgctgctg gggctgttct tcatcaccat cggcatgctc
    10861 ctggactggc gcatcgtggc ccagcactgg tggctggtgc tgctgctggt gacgctgccg
    10921 gtgctcttca agctgctcct ggtggccggg ctgacgcgcg cgctgggcgc gtccgccggg
    10981 gtgtcgctgc gcaccgggct gtacctggcc caagcaggcg aattcggctt cgtgctgctg
    11041 accctggcca accagcagga actggtgccc ccggtcctgc tcaatccgat cctcgcgagc
    11101 atggtgctgt cgatgctggc cacccccttc atcatcatgt actccaaccg catcgtgatg
    11161 aagctggtgt ccagcgagtg gatgctgcaa tcgctgcaga tgacgaatat cgcgcgcaag
    11221 tcgatcaaca ccaaccggca cgtgatcatc tgcggctacg ggcgctgcgg ccagaacctg
    11281 gcccgcatgc tggagcacga gggcatcccc tacatcgcgc tggacctgga ccccgaccgg
    11341 gtgcgtcagg cgacggcggc cggagactcg gtggtgttcg gcgacgcctc gcggctgcag
    11401 gcgctggtgg ccgccggcct ggcgcgcgcg agtgccgtgg tggtgaccta cctggacgtg
    11461 cccggcgcct tgaaggtgct atcccgggcg cacgaccatg cgccccaggt gccggtgatc
    11521 gttcgcaccc aggacgatcg cgacctggag gagcttcagg cggccggcgc gaccgaagtg
    11581 gtgccggagc tgctggaggg ttcgctgatg cttgccagcc atgcgctggc cctggtcggc
    11641 gtgccgatgc gccgggtgat ccggatcgtg caggaccagc gcgatgcgcg ctacaacctg
    11701 ctgcgcggtt acttccacgg ggccgacgac gacaccgtga gcgagctcga gaacgagcgg
    11761 ctgtcgaccg tgaccctgcc cttcggcgcc ggctcgctcg gccacgccct cagccagatg
    11821 gcactccagg cgctcgacgt gcgcgtgatc agcctgcggc gcagcaacgg caaaccggtc
    11881 gatcccgaca ccgacccccc gctggaggac ggcgacacgc tggtgctgtc cggccggccc
    11941 gagaccctgg cgatcgccga ggaaaaactg ctgcacgggt gagagacggc gccgccgcct
    12001 cgccgcctca gtcgttcacg tgaactggcc caccaggagg gcttcgcgca tgcgcaccgc
    12061 cagcacggcc gccaggccct tcaccaggtc cagcgccagc cggggatgct tgctggccat
    12121 cgcgtcgaat ttgtcccggt tcatccccca ggccacgccg ggttgcgacg gcgtgacggt
    12181 ggcgctgcgc ggcgcgccgg aaaaaaaggt gccttccccg accacgctgc ccgccttgag
    12241 cgtggcgacc ggcgtgccca ggatggtgac ctccagctcg ccgtcggcca ggaagaacag
    12301 ctcgcgttcg cagtcgccct cgtgcatgag cggatcgccc ggcttgagga aacgcatcga
    12361 caggtagggg gcgagcgcct tccactcgtc gagcgtgagc ttgcactgca gcgcgtccgt
    12421 ggcgtcaagc gccgcgatcg cccgcaccag ctgttccacg ttcatggtgt ctcctcaacg
    12481 gccttccccc gggtcatgcc gcagtgtata gcccggcccg cgggccacgg cggcggggga
    12541 acgcgttcag cgccgccgcc gcgtcagttc aagtcgccgt actgggtacc gctgagggag
    12601 ggcatgcgct ggtcggtatc cggcatttcg gtgtcctcga agccggtgag cagggtatag
    12661 ctttgcggcc catgcatggc cgagccgtcg aaggacttgg cgctggcgcc atgggaaacg
    12721 ccgacggccg ccgcggccgc agccgccggg ttgctcacgc ccgccgccag tgcccgcttg
    12781 aatgccgcca cctcgtcggc ctggatcggc tcgtagcgcg agagggtccg cggcggcgcg
    12841 gtgggcgcca gcgggacggg gccggagtcc agcggcgtcg gctgcgaagc cagcgcgtcg
    12901 ggctccgggt cgagcggcac cgggcgcgag tcggtccccc gctgcagggc gacgccgggg
    12961 tcgccgatgg ccacatgctc gttgctgcgc cagtacacgg ccgtgaccag gatgtcgtgg
    13021 cgggacttgg cggcctgggc gatcagcgcc tcgatctcgg ccagccgcgc cgtttcgccg
    13081 ccgttgtcgc gcgcaaggtc caccatgacc aggaactggc ggccgcgcgc gtccagcgaa
    13141 agcaccttga acttgtagct cgccgacagc acgccggcgc gcatcatgga ctcgcgcacc
    13201 acggcgtaaa gcagctcgcg ccgggccatg cgctcgctct tgcgattggc cggctggccg
    13261 ttggcggcca ccggttcact gccggcctgg cgtttcatca ccggttgggt gggctctatc
    13321 cgggacagcc cggaactctc ggcctggacc ttggccggcc ggaaggccgg gatcaaccag
    13381 ctgaacaaag acatctcggg ctttctgcta tgcgggtaag gggccgtgga acgcacactt
    13441 tcgttccgtg cggcccgtta gtgtaaatct ggcgggcgta aacaccgcca acttttgcgc
    13501 gcccgtggca gcgcgtggcc gccgcgtgcc tgacttcgtg aaaaagtagc cgcgggcagg
    13561 ggtggcgcgg caggccactc acgtcaccgc gacccgtttg ggcttgttgg ccagccggcg
    13621 cgacaccagc gagcccaacg ccatggcgat ttccagggcc atggcgggct ggcggttggt
    13681 cagctcggta aagcggatcg gcgtcaggca ccagaccttg ccggggctgg cggactgcac
    13741 cgtggcgctg cgcggaaggc gcgtgaaaaa cgcgccttct cccacggccg aaccgggacc
    13801 gaccagggcc aggcgcaagc ggcccttgtc gtcctcgtaa tgcacggtca agctgcccga
    13861 ctcgaccagg tacacgttgc ggtcctgcgc gttctgttcg atcagcacct gcccttgcat
    13921 caacgcgaac ggctgcatgt aggtccccag caattcccac tgggtcgccg tcaacgcggg
    13981 cgagaagctg tcactggatg ggctctcggc aatcgccttg accaaacctt tcacatcgaa
    14041 ccggaggggg gcgcgcaacg ctacgttcat gaaacacgac agtagccgcc tgctgagcca
    14101 cgcacaaggc accctaacgg ccacttacac ctgatacccg gctccaaacc gtgtaaccgc
    14161 cgcccggcgg aacccgccga aagctcaggt cacggccacc agcggatcgg ccttcttgcc
    14221 gatgcccagg taggtgtcga tgatgcggcg gtcgtgcagc agcgcctggg ccgtgccgct
    14281 caacgcgacc gcgcccattt ccagcacgta ggcgcggtcg gccacctgca gcgccgcgcg
    14341 cgcgttctgt tccaccagca gcaccgaaac gcccagcttg cgcagcgagg ccaccatttc
    14401 cagcacctcg cgcacgatca gcggcgccag gcccagcgag ggctcgtcca gcatcagcag
    14461 gcgcggctcg gccatcagcg cgcgtcctat ggccagcatc tggcgctccc cgccggacag
    14521 ggtggaggcc tcctgcgccc ggcgctcctc caggcgggga aacagggcga acacctcctg
    14581 catgcgccgc gcctggtcgc gctggccctt gcgccagcgc cagaagccgc ccagcagcag
    14641 gttgtcttcg accgacattt cgccgaacag ctcgcgccgc tcgggcacaa gcgccacgcc
    14701 gcgcgccacc atcgcctcca ccgtgggcct ggcaatggtg tgctcgtcca gcgcgagcgt
    14761 gccgctggac ggcagcaagc ccatgacggc gcaaagcagg gtcgacttgc cggcgccgtt
    14821 gggcccgatg acggtgacga tctcgccttc tcgcacgctt agctcgacgc catggacggc
    14881 ctgcacggca ccgtacgaaa cgcagaaatc gcgcagttgc agcagggtgg cgctcatgcg
    14941 gcgacctcca gcggctcgtc ggcgccgccc aggtaggcgt ccagcacgcg gggatcggcc
    15001 tgcacctggg ccggggtgcc gtgagcgatc acggtgccga attccaacac tgtcacgcgg
    15061 tccgccaggt tcatcacaaa ctccatgtcg tgttcgacga tcaggatgcc caggccttcg
    15121 gcccgcagct gcgccagcag ttgggccagc gcgactttct caagatggcg caagccggcg
    15181 gccggttcgt ccagcagcag cagcgccggg tgccccgcca gggcgcgcgc gatttcgatg
    15241 gcccgctgct tgcccagcga cagggaaccg gccggcgcat tcacatgggg gccgagctgg
    15301 caacgctcga tctggcggcg cgcctgcgcc agcagcgcgg cttcttcggc ccggtccagc
    15361 cgcagcatgg aggccagcca gccgcgccgg ccgcgccggt gcgcaccgag cgcgacgttg
    15421 tccagcacgc tgcgctggcc cagcagccgc acatgctgga aggtgcggcc caggcccagc
    15481 gccgcatgct cgcgcgcggg gtggccggtc atgctctttc ccatgaggct gacttcgccc
    15541 gagctgggat cgatgacgcc cgagaccatg ttgaagaagg tggtcttgcc cgcgccgttg
    15601 gggccgatca gcgcatgcac ctcgccggcc acgacatcca ggctcacgga attgacggcc
    15661 gtgagaccgc cgaagcgttt gctcgcctga ctggtgcgca gcagcacctg gccggccggc
    15721 ggcagctgca gttccggcag cggttcgctg tcggggcgca ccgctgtcga atcgggccgc
    15781 aggaagcggt gcgagacggc agccagcgtc ggccacagtc cgtcggcgaa ccggtgcagc
    15841 accaccagca tcagcaggcc gaaggcgatc acttcgaaat tgccgctgct gccgagcaat
    15901 tgcggcagca catcctgcag ccgggacttc agcagcgtga ccagggccgc gcccagcacg
    15961 gcgccccaca ggtggcccgc gccgcccacc accgccatga acaggtattc gatgccgatg
    16021 ttgaggttga acggcgtcgg attgacgaac cgctgcaggt gcgcatagaa ccagcccgag
    16081 agtgccgcca gcagcgcggc cagcacgaag atcttgaccc ggtaggccgc cgtgtccacg
    16141 cccatcgatt cggccatgag gcggccgccc ttgagcgcgc gaatggcgcg gccttcgcgc
    16201 gagtcgagca ggttgtgcaa cgcccacagc gccagcagca gcgtggccca gatcaccgga
    16261 ccgagcgccc gcggcgccgc cagcgacagc gagccgacca tcaggggcgg cagtccgccc
    16321 gtgccggtct gtccgcccag cacttcgagg ttgccgaaca ggaaatagat gctcaggccc
    16381 caggcgatgg tgcacagcgg caggtagtgg ccggacagct tgagcgtgat cgcgcccagt
    16441 ccccaggcca cggccgccgc caccaccaag ccaagcagca agcccagcca cggcagccat
    16501 atcggggcga tcagtccgcc cacggcagcg gcgaacgctg gcgaagtggt gacccaggcg
    16561 gtggcatagg cccccacgcc cacgaatgcg gcttggccga aggaggtcat gccgcccacg
    16621 cccgtcagca tcaccaggcc cgccgccacc atcgaataca ggccgatgta gcacagcagc
    16681 gtgatggtga attcgggcag ccagggccat gccacggcca atgccagcac cgccagcaga
    16741 gtgagtccgc gccgcgtcat tattcgtctt cttccacgtg gtggctgtgc agcgaacgcc
    16801 accacagcac cgggatgatc agggtgaaca ccagcacttc cttgtaggcg ctggcccaga
    16861 acgaggagaa ggattccagc tggcccacca gcagggcgcc gcccagtgcc agcgggtagc
    16921 tggccatgcc gccgacgatg gcggccacga agcccttcag gccgatcagg aagccagtgt
    16981 cgtaatagac ggtggtgagc ggcccgatca gcaggcccga cacggcgccg atcaggccgg
    17041 ccagcgcaaa gctcaggtca ccggagagct cggtcggaat gcccatcagg cgggcgccga
    17101 cgcgattgat cgccgtggcc cgcagggcct tgcccaccat ggtgcgcccg aagaagcgga
    17161 acatcgcgat caccagcacc agcgtcacgc ccaccaccac cagcgactgg ccggtgacgg
    17221 ccagaccgcc caggtcgaga cgaagatcgg aaaaagccgg ggtgcgcgaa ccttcggccc
    17281 cgaaaaagag caggcccagc ccgaccagca cgccgtgcag ggcgaccgac acgatcagca
    17341 gcatcagcac actggcgtcg gccaacggcc ggtacgccag gcggtacagc agcggcccga
    17401 gcggcgtcac caccaccaag gtggtgagcg cctgcaacgc cagcgagctc ggccgcagca
    17461 acaccagggc gccggccagg gcgggaaaca gcacggccca gaccaggctc gaagcccagt
    17521 ccaccgccgc gccgcgccgc tgccgccagg cttccaccag caaggtggcc acggccaggc
    17581 ccagcagcag ccacagggtg cgcggcgcgc tgcccgagtt cagcgccgcc atggacagcg
    17641 cggcgaacgc caccagttca ccttgcggaa taaagatgat tcgcgtgacg gaaaacacca
    17701 gcaccagcgc cagccccatc aggccgtaga tggcgccatt gactatgccg tcctgcaaca
    17761 gcagcagggc gatctgaaga tccatgatca ataccggttg cgggccttcc aagggcctca
    17821 gattgaagac ggggcgccca tgcggggcgc cccttgcgct ttcagggcag cgcgcgcgcc
    17881 gccttcactt cagtgcgtcg acgttcaggg cgtgtatttc caggtgccgt tttcgatctt
    17941 gaccatcacg cgtgcattct tgtccaggcc cagatggtcg gtcttgctca tcgtgaagat
    18001 accgtgcgcg ccggccactc ccttgacttg ctcgatcgag tcccgcagcg cagcgcggaa
    18061 ttccgctgtg cccggctggg ccttcttcag ggcgccgggt atcgcggctg ccatcagcat
    18121 gccggcgtcc caggcgtggc caccgaaggt cgacacgctg cccttgccgt gggcgccttc
    18181 gtacgcggcg atataggcca tcgcggactt cttgacaggg tgactgtcgg gcagctgatc
    18241 ggccaccagc accgggccgg cgggcagtat cgtgccctcg acgtccttgc cgcccacgcg
    18301 caggaagtcg gcattggcga tgccgtgcgt ctggtagatc ttgccggcga aaccgcgctc
    18361 cttcagtgtc ttttgcggca gcgcggcggg tgttccggag ccggcgatca gcaccgcgtc
    18421 gggcttggcg gcgatgagct tgagcgcctg gccggtgacc gaggtgtcgg tgcgggcata
    18481 gcgttcattg gcgacgatct tcaggttctt ggcggcggcc gccttggtga attcctggtt
    18541 ccagccctcg ccgtaggcgt ccgagaagcc gatgaaggcc accgtcttca cgccattggc
    18601 ggccatgtgc tccacgatcg cctgcgacat catgatgtcg ttctgcggcg tcttgaacac
    18661 ccaggccttc ttggcgtcca cgggctcgac gatcttcgcc gaggccgcca tggagatcat
    18721 gggcgtcttc gcttccgcca ccacgtcgat catggccagg gagttgggcg tgatcgtgga
    18781 acccaggatg acgtcgacct tgttttcagt gatcagcttg cgggtgttgg tcacggcggc
    18841 cgtcgtgtcg gaggcgtcgt cgagcacgat gtagttgatc ttctgcccgg cgatggtctt
    18901 gggcatcagc gcgatcgtgt ttttctcggg aatgcccagc gatgccgccg ggcccgtggc
    18961 cgacaaggtg acgcccacgt tgatgtcggc cagcgccagg gtgctggcca gcagggctgc
    19021 cgccaatgcg acgggtttca gtagtttcat gccgagtctc cggatgtagt tgagggaaaa
    19081 gggtaaccga gtaccaaaat acatcatgac ggtcccatgg ggctcagggc ttacccggcg
    19141 gttgggcttc cgatgtgatc tagctcaaat aacctaaccg gtcggtcggg gattgttgtt
    19201 attttccgat gcagaagctc gagaagatga cccccagcag gtcgtccggg gtgaattcgc
    19261 cggttatttc attcagcgcg ttttgcgcca gccgcaattc ctcggccagc agatccagcg
    19321 cctgggcctg cgccgccagg tgccccgcgg cctccagcag atgggcctcg acccggcgca
    19381 gcgcctggac atggcgttcg cgcgctatat agatgccctc gggcgccgcc tgccagcccg
    19441 ccgcctccag cagccggcgg cgcagcgcgt ccaggccggc gccggtcttg gccgagagca
    19501 agacgctgtc tgcggcgctg gccgccgccg ccaccccggg cgcaaccagg tcgagcttgt
    19561 tccagacttc gatcaccggc accgtgcgcg gcaatttgtc ggacaggccg cgggcgatcg
    19621 cttcatcccc gaccgcgtaa tcagcatctc ttgcccgagt caaatcgtgc aggaacagca
    19681 cggcgtcggc attttcgatc tggccccagg cccgctcgat gccgatcttc tcgacatggt
    19741 cctggctgtc gcgcaggccg gcggtgtcga tcacatgcag cggcacgccc tcgatctgga
    19801 tcgccccgct gaccacgtcg cgcgtggtgc cgggcaccgc cgtgacgatc gccagctcgg
    19861 cgccggccag cgcgttgagc agcgaactct tgccggcgtt gggctggccg gcgatcacga
    19921 ccttgatgcc ttcgcgcagc agcgcgccct ggcgcgcccg ctgcagcacg cgcgccaggg
    19981 tctggcgcag ccgctccagc tggccctggg catcggcctt ctgcaggaag tcgatctcct
    20041 cgtccggaaa atccagcgtc gcctcgacca gcatgcgcag gtggatcagc gcgtcgcgca
    20101 atgcgtggat ctcgcgcgag aactcgcccg acagcgaccg gctggcgctg cgcgccgccg
    20161 cttcggtgct ggcgtcgatc aggtcggcga tggcctcggc ctgcgccagg tcgatcttgt
    20221 cgttgaggaa tgcgcgctgg gtgaattcgc cgggctcggc cacgcgcagc ccttccagcc
    20281 tgggcttgcc ggtggccgga tcggcctcgc cgccggcctc caggcagcgc gccagcagca
    20341 gctgcagcac caccgggccg ccgtgcgcct gcagctccag cacgtcctcg ccggtgtacg
    20401 aatgcggcgc cgggaaatgg atcgccaggc cctggtcgat gatgctgccg tcggcggcga
    20461 gaaaaggcag gtaggtggct tcgcgcggct tgagcgcgcg gccgcacacc gcttcgatca
    20521 gcgcggcgag cgacgggccc gacacccgca cgatgccgac cgcgccgcgg cccggagcgg
    20581 tggcgatggc ggcgatgggc tgggtgtggc gcgcgagcat ccccgcatcg tagcggggcg
    20641 tggcgcctca acggaccagg gcggtggacg cggccagccc atgcacgagc atgcggctgg
    20701 agcgatcgct gaggtaggtg tcgccggcct cgatcaggat gtccttgatg ccgccgtcat
    20761 gcgtgatcca gaggctgccg ctcacgcagg tgacgttcgc gcccagcgcg cgctccacta
    20821 ccagcgtagc gcccttgtcg atggacatgg ggccttgcaa cgccggcacc acgcgatggg
    20881 ctgcggcccg gcggggcagg cgcacagcac ggaaccagct ggacaacgga aaggcggcga
    20941 gcgggttgag ggcgatggtc atgctggtct ccagggaagt tgttcgatgg agcaagtatg
    21001 tttttcgccg gccccggccg gtagtgtgcg gaatacggta acggcgggcc gcaactacgg
    21061 tagggggtac cgtagccggg ggccgcggct caaggcgcca gcaggccggc ctgagcggcg
    21121 cggtgcaccg cctcggcccg cgtcgcgcag tccagcgctt tcatggcgga ggccacgtgc
    21181 atctcgaccg ttcgcggact gaggctgagt gcgcgcgcga tctgcttgtc tgtgaggccg
    21241 gccgccacct gtttcagcac ctcgtgctgg cgggccgtga ggcgcgtgcc ctcggcccgc
    21301 gctttcaccg gctcgccaag gaaggcgcga agctccagca cgacctccgg ccaacacggt
    21361 tcgcgggcga gcggaaggtg atttctgctg ttcaccggca ccaggcgggc acccggaatc
    21421 aggccggcca gtttgcgtcc ctggtcgaaa tagatcatct ggtcatcgcg ggcgtggaac
    21481 acgatggccg ggcactgcac cttgcgcgct tcttgcttca cgttgacgag atacatcgcg
    21541 cgcaagcagc gcactgcgac gtcgggcgtg gcggtgagat tctggtattg atcgaactcg
    21601 tgctgctcgg ccgcggtgct gtcgggcatg agtttgctca caaagacctg gcggaaggct
    21661 ggtgagccct tgccccatcc cagctcgacg agtttcagca acgtttcgcc ctcgtcgacg
    21721 atcttcgggt cgggattgcc ggtcgtgaaa taggaggtgg caaagccgcc ccataacacc
    21781 atggcgctca cacgatcggg gtgccgtgcg gcgtaggcca cggcgatcgc cgcgccctgc
    21841 gatatgccca gcaaggggaa acgctccagc cccagcgaat cgacaacggt ctccaggtcc
    21901 cgtacccagg cctcgaagga aatatcggcg acattgcgcg aggacaagcc gcaaccgcgc
    21961 gcgtcataag tcacgtagcg atggcggcgg ctcagctcct cgatccagtg cgggttgagc
    22021 acgctcgtcg gatcggcctc gatatgggtc agccaggtcg cggctttgac caggggcggg
    22081 ccgtcgccat acagcgccat ggcgagctgg acgccatcgg ccgtgctgca gaagcggatg
    22141 tcctgatgga tcgacatggc gccggctcct tcgagcggac cagtgtaagg ggctggcgaa
    22201 cccgcggcaa tgctgacaag gcagcaaaag gcatggattg cgggtcaagc ccgcaatgac
    22261 agggggcttt gtcattgcgg gctgggctcg cggaacgccc ggggcgcgca gctgtccagt
    22321 gcagcgagcc caatgccggg cctacgctat gcacgtaccg aggcatcacg ctccggcccg
    22381 gctccgcaat ccaggggttc tacctgaact tcggcaggtt gaactgcggc ggcacgccca
    22441 tgcgcgtgtt gatcagccac tgctgggcga tggacagcac gttgttcgtg atccagtaca
    22501 gcaccagccc cgacgggaag aaaaagaaca tgatgctgaa cgccagcggc atgaaccaca
    22561 tcagcttggc ctgcatcggg tccggcggcg tcgggttgag cgccgtctgc agcagcgtgg
    22621 tcagcgtcat caccaccggc aggatgaagt acgggtcggg cgtggacagg tccctgatcc
    22681 agccgatcca gggcgcgccg cgcatctcca cgctggacag cagcacccag tacagcgcga
    22741 tgaacaccgg gatctggacc atgatgggga agcagccgcc catcgggttg accttttcct
    22801 cgcggtagat gcgcatcatc tcctgctgca tctgctgcgg gttgtccttg aagcgctcgc
    22861 gcatcgccat gaccttggga ttgatggcct tcatcttggc catgctctcg taacccttgg
    22921 cctgcagcca gtagaaggcg atcttgatga tcagcaccag cgccatgatc gaccagcccc
    22981 agttgcccag gacatcgtat atcttgtcca tcagccagta gagcggcttg gacaggatgg
    23041 tgagccagcc gtagtccttc accaggtcca ggcccgtcgc gatctgctcc agctgctttt
    23101 caacctgcgg gccggcgaag agcttcgcat ccacggccct ggcaaggccg ggcgcgatcg
    23161 gctcgatcgg cgcgatcatg ccgacagcgt acaggttggt gtcgactttg cgcgcgaaca
    23221 gttcgcgctt gacgccgtcc ggcaggatcc aggcgctggc gaaatagtgc tgcaccatcg
    23281 ccacgtagcc gtcgctggat tgcttgtcga tgtcgacctt gcccttttcg atgtcgctga
    23341 actccacctt gctgaatttc ttggcctcgg tgtaaaccgc cgggccggtg aaggtgaaat
    23401 agaacgagga ctcccccggc accttgttgc cgtcccgcac cagctgaagg taaagctgcg
    23461 gcgagaccgg ggcggtgccg gtgttcagca cttcgtggcg aacgacggcg tcgtaagcac
    23521 cgcgtttgaa ggtgtagctc ttgaccagct tgacaccgcc cttttcgggc gactcgaacc
    23581 gcacgaccag ctcgttggcg cccgccttca gttcccgctc ggcgctcggc gtcatcactg
    23641 tcttgtggtt ggggaaatcg ccgccgatca agccggtttc ggccacgtac ttgtagcccg
    23701 ggcgttcgtc cagcagcacg aaggtgccgg tcttgttttc gccggcctgc ttgaggaatt
    23761 cggtccgcac cagcgagccg ccctgcgtgt cgaaggtcag gtgcagcagg tcggtggtga
    23821 cggtgaattt ctcgccgacg gccgaagtca tcgccggagt ggggggcacg gcgccgggca
    23881 ctgcggcagg cgccgtggcg gtgggggcgg gcaccgtgct ggccggcggc acgccggacg
    23941 gggcgggcgc gcccggggcc gtggctgccg gggggatggg cgtgctcggg aagaaggtcg
    24001 gcttgctgcc gttatagatc tgccactggt cccacagcaa caccatggag aaaccaaaga
    24061 tcacccacag gatggtgcgg cgaatgtcgt tcatgaagac ttcttcttcg aaagagaggg
    24121 gagcaggtgg gtgaagagcc gcgtgagcgt gggggcttgt tccggcactt cgtcatggcc
    24181 gccctcgcac caggggtggc agcgcgccag gcgcgccagg gtcaggtagg tgccggcggc
    24241 ggccccgtgc cgctccagcg cctgcagcga atacgcggaa caggtgggcg tgaagcggca
    24301 gctcgatccc agccacgggc tgagcaggag gcggtagccc ttgaccaggg caatgagaag
    24361 ggcgcggatc attgcgcggc ctgcgcgaac agttgttgca gttcggcgcg cacggcgcgc
    24421 ttgagcgcgc cggatgtagc actgatgaac tgcgtgcggt cgaagcccgc gcgcaagcgc
    24481 accacatggg cggcggcggg cagcggcggc tggagttcct ggctcacggt atagatctgt
    24541 cgtttgatgg cgttgcgtgt cacggcccgc ttggcccagc gcttgggcac cagggcgccg
    24601 atccaggagt ctcggaaagc aaacagggcc ttgggtcctg cggaccccgg ccccatgggc
    24661 gcatttgaaa agcgtcctgc ggacgcgggt cccgcggacc ccggccctgc gtcaggcgcc
    24721 ggcggcgcat cgagcggcct gcggtgcaac gcgaagtgcg cggtacggga gacagtggtg
    24781 cccgccatga cggcctcgaa ctgtgcccgt gtcttcagcc gctgcacttc aggtgcgccg
    24841 catgggccgg cccgcaacga gccggctcag acggccaggc gcttgcggcc cttggcgcgg
    24901 cgtgcgttga tgacggcccg gccgccccgg gtcttcatgc ggaccaggaa gccgtgggtg
    24961 cgtgcgcggc gcactttgga gggttggtag gtgcgtttca tgttcgtatc tcagggaaac
    25021 ccatgattat catggaattc atggaatttc tcaatagtgg caaggacata gcctcgtaag
    25081 aaggtcattc ccgtcacccc ggacttcaga aggctgtcat cccggactgt catcccggac
    25141 cccggatcaa gtccggggca ggcccgatcc gggatccagg actcctgaat tcatggattg
    25201 cgggtcaggc ccgcaatgac aagtctctgg gtctgacaag tctctgggtc tgacaagtct
    25261 ctgggtctgt caagtctctg ggtctgtcaa gtctctgggt ctgtcaggcc cgcaatgaca
    25321 aatctctgtc tgtcaagccc ccaacggcat ccgctgcatc tcggctttat cgcgaaatcg
    25381 tctatgtgga taaggccttg ataaactaga atccaaggct cccagaatga ataatatcca
    25441 cagaggcgac agccaataat gagcgagggg caacacaaca gcctggcggc tcacgccggc
    25501 atcggtgccg gcgacagcct gtggcaggcc tgcatcgacc agctggcgca ggagctcccg
    25561 gagcagcagt tcaacacctg gatcaagccc ttggtggccc aggtttccga tgacttctcg
    25621 cgggtcacgc tgttcgtggc caaccgcttc aagctggact gggtgcgggc gcagtacagc
    25681 agccgtatcg ccggcaccct ggaaaagctc tatggccagc cggtgcatct ggagttagcg
    25741 cttgctctgc gtgaatctcc ctccaggact tactctagtc ctgcctcctc cgagatgggc
    25801 gcggcccccg agccggccga gatcagcgac gacggcgccg ccgccgcgtt caagaatcga
    25861 ctgaacacgg ccctgacttt cgacaccctg gtcgagggca cggccaaccg catggcgcgc
    25921 gccgcggcca tgcatgtctc gaccacgccc ggccatcttt acaatccgct tttcatctac
    25981 ggcggggtcg gcctgggcaa gacccacctg gtccacgcgg tgggcaaccg gctgctggcc
    26041 gacaagcccg gcagcaaagt tctctacatc catgccgaac agttcgtctc ggatgtggtt
    26101 aaggcctacc agcgcaagac tttcgacgaa ttcaaggagc gctaccactc gctcgacctg
    26161 ctgctgatcg acgacgtgca gttcttcgcc aacaaggacc gcacccagga ggagtttttc
    26221 aacgccttcg aggccttgct ggccaagaaa tcccacatcg tgatgaccag cgacacctat
    26281 cccaagggcc tggccgacat ccacgagcgg ctgatttcgc gcttcgactc cggcctgacg
    26341 gtcgccatcg agccgcccga gctggaaatg cgcgtggcca tcctgatcaa caaggcccgc
    26401 gccgaaagcg cagaaatgcc cgaggaagtg gcgtttttcg tcgccaagaa cgtgcgctcc
    26461 aacgtgcgcg agctcgaagg cgcgctgcgc aagatcctgg cctattcgcg cttcaaccag
    26521 aaagagatct cgatcatgct ggcgcgggag gccctgcgcg acctcctgtc gatccagaac
    26581 cggcagatct cggtggagaa catccagaag acggtggccg actactacaa gatcaaggtc
    26641 gccgacatgt actccaagaa gcgcccggcc tcgatcgccc ggccgcgcca gatcgccatg
    26701 tacctggcca aggaactgac ccagaagagc ctgcccgaga tcggcgagct gttcggcggc
    26761 cgcgaccaca ccacggtgct gcacgcggtg cgcaagatcg gcggcgagcg ccagcagatg
    26821 accgaattga accagcagct gcacgtgctg gagcagacgc tcaagggctg atgatgcccg
    26881 ctgagagcac caggaattgt catgccggac tcgatccggc atccaggggc ggttgtcatg
    26941 ccggacttga tccggcatcc aggacttcct ggattgcggg tcaagcccgc aatgacaacg
    27001 acggggcgcg caatgacagc caacggcgtg tcagccagcg tgtcagccag cgtccggatt
    27061 ggcttacggg gcacccctct aacaaccctg tggagcgttc cggcacaact cggcagttat
    27121 ccaccgcatc ggcgaaaatg ggaagttgtt cattccggcg cgacaccacg gaagcagggt
    27181 ggcgcacagt gtttggggtt cggcaagtgc ttgatgaaaa aggggaaaat agcggttgtc
    27241 cacagacggg ccgccccctt atctagctac tactagattg aattaaagaa attaaagaca
    27301 gaagaggtag acatgatcgt cctgaaggca acgcaagaca aact
//
LOCUS       DASWIY010000142         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_79034, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000142 DASWIY010000000
VERSION     DASWIY010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79034"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VGA59_05450"
     CDS             complement(<1..506)
                     /locus_tag="VGA59_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX9719157.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     ELPDRRLGQLAAIAKPERIVPAIVEFVDIAGLVAGASKGEGLGNQFLAHIRETDAIVN
                     VVRCFDDPNIVHVTGKVDPVSDVEVIQTELCLADLGTVDKTMQRSIKAAKSGNDKEAA
                     KLVQVLIKV"
     gene            538..>2341
                     /locus_tag="VGA59_05455"
     CDS             538..>2341
                     /locus_tag="VGA59_05455"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX9719158.1"
                     /translation="MRCLAGQAWTACRGALVLIVALACAAAAVAEVRPVPATAVLSES
                     SATMDAQGLGLSWIDASGTATLEQVLQTGAAFQPARPDLIYSLGEHGALWLRYRLLRG
                     REERHNWLMAFPMPALDRVTVYQQDAKGRWLGQTAGDTMAVSAWPEPGRYPHFRLDLP
                     VGQARDVYVHIRHFTHANFPIQLLTEAAYDQRVQLEYLGLGLTFGALLLLIATCLAQS
                     LVYRDRTYAWYALYALVTALAVASYTGAAAHLLWPGFAALGDSPQGCLAMLGSAAALL
                     FVRNLTGISARHPVVDRMVYAAGLGGLLLAAAFLVLDKPIGLIALAVYVAATSLTNVW
                     IASAGWRRGDVVGAWVLVAYSPFALAVCLAIVRLFGWLPVSFGTQYAVVAALALQVPL
                     LLVALNIRSRERHGAEIRELALSSQDALTGLLAPHLFHDRLRQVVARYKRDKENAAVV
                     FIDLVNYPRIKDYYGTAVAEQSLLRCVIKLRRLLRDVDTVSRIGEARFGLILEGVTSR
                     IAVTDRAARLIAAGLMPLQGLKPDVTLQFHIAAVLLEERISEAPELADALGELLATMS
                     PRTRRPIRFLEPEVTLPASMEPESDSQLLESESLP"
BASE COUNT          338 a          798 c          785 g          420 t
ORIGIN      
        1 actttgatga gcacctgcac cagcttggcg gcttccttgt cgttgccgga cttcgcggcc
       61 ttgatcgagc gctgcatcgt cttgtccacc gtgcccaggt cggccaggca cagctcggtc
      121 tggatcactt cgacatccga caccgggtcc accttgccgg tgacgtggac gatgttgggg
      181 tcgtcgaagc agcgcaccac gttgacgatg gcatcggtct cgcggatatg cgccaggaac
      241 tggttgccca ggccctcgcc cttgctcgcg cccgccacca gcccggcgat gtcgacgaac
      301 tcgacaatag ccggtacgat gcgctcaggc ttcgcgatcg cggcgagctg gcccaggcgc
      361 cgatcgggca attcaacgat gccgacgttg ggctcgatgg tgcagaaggg atagttttcc
      421 gctgcaattc ccgccttggt caacgcgttg aacaaggtgg atttccctac gttgggcaga
      481 cctacgatcc cgcacttcaa gctcatggca gttcccgaaa atcaagccgg aaagtgtatg
      541 cgatgcctcg ccggccaggc ctggaccgca tgccgcggcg ccctcgtgct gattgtcgcg
      601 cttgcgtgcg ccgcggcagc ggtggccgaa gtccggccgg tgcccgccac ggcggtgctg
      661 agcgaatcga gcgccacgat ggatgcgcag ggtctgggcc tgtcctggat cgacgcgagc
      721 ggcacagcca ccctcgagca ggtgctgcaa acgggcgcgg cattccagcc ggcccgcccc
      781 gacctgatct actcgctggg cgagcacggc gccctctggc tgcgctaccg gctgctgcgc
      841 gggcgcgaag agcggcataa ctggctgatg gcttttccga tgccggcgct cgaccgggtc
      901 accgtctacc agcaggacgc gaagggccgg tggctgggcc agaccgccgg cgacacgatg
      961 gcggtgtcgg cctggcccga accgggccgc tatccgcatt tccggctcga ccttcccgtg
     1021 ggccaggcgc gtgacgtcta tgtgcacatc cgacacttca cccatgccaa tttcccgatc
     1081 cagctgctca ccgaagctgc ctacgaccaa cgcgtccagc tcgaatacct gggcctgggc
     1141 ctgaccttcg gcgccctgct gctgctgatc gccacctgcc tggcccagag cctggtctat
     1201 cgcgacagga cctacgcctg gtacgccttg tatgccctgg tcacggcact tgcggtggcc
     1261 tcgtacacgg gagcggccgc gcacttgcta tggccgggtt tcgccgcact gggcgattca
     1321 ccccagggtt gcctggccat gctgggctcc gccgcggcgc tgctgttcgt gcgcaacctc
     1381 accgggattt cggcacgcca cccggtggtc gacaggatgg tttacgcggc cgggctgggc
     1441 ggcctgctgc tggccgcggc gttcctggtg ctggacaagc cgatcggcct gatcgcgctg
     1501 gcggtctacg tggcggcaac gtcgctcacc aatgtatgga tcgcctccgc ggggtggcgg
     1561 cgcggggatg tggtcggtgc ctgggtcctg gtggcctatt cgccgtttgc cctggcggtg
     1621 tgcctggcga tcgtgcgcct gttcggctgg ctgccggttt ccttcggcac gcaatacgcc
     1681 gtcgtggcgg cgctggccct gcaggtgccg ctgctgctgg tggcgctgaa catccgttcg
     1741 cgcgagcgcc acggcgcgga gatccgggaa ctggcgctgt ccagccagga cgcgctcacc
     1801 ggcctgctgg cgccgcacct gttccacgac cggctgcgcc aggtggtggc ccgctacaag
     1861 cgcgacaagg aaaatgccgc ggtggtgttc atcgacctgg tgaactatcc gcgcatcaag
     1921 gactactacg ggacggcagt cgccgagcag agcctgctgc gctgcgtcat caagctgcgc
     1981 cggctgctgc gcgacgtgga caccgtcagc cgcatcggcg aggcccggtt cgggctgatc
     2041 ctggagggcg tgacctcccg catcgccgtg accgacaggg ccgcgcggct gatcgcggcg
     2101 gggctgatgc cgctccaggg cctgaagccc gacgtcacct tgcagttcca tatcgcggcc
     2161 gtgttgctgg aggagcggat atcggaggcg cccgaactgg cggacgcact gggcgaactg
     2221 ctggcaacga tgtcgccgcg cacccgccgc ccgatccgtt tcctggagcc cgaagtcacc
     2281 ctgcccgcgt ccatggaacc ggaatcggac tcccagttgc tcgagtccga aagcctgccg
     2341 c
//
LOCUS       DASWIY010000143         3516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_79088, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000143 DASWIY010000000
VERSION     DASWIY010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79088"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..473)
                     /gene="ssrA"
                     /locus_tag="VGA59_05460"
     tmRNA           complement(89..473)
                     /gene="ssrA"
                     /locus_tag="VGA59_05460"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(488..1162)
                     /locus_tag="VGA59_05465"
     CDS             complement(488..1162)
                     /locus_tag="VGA59_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX9719159.1"
                     /translation="MFSDIEAVLFDLDGTLIDSAPDLGAAADTMRVARGLPSLPLELY
                     RPMAGAGARGILGVAFGMTPDHPDFAALREEFFINYENCMTQRTHAFEGVAELIAQLL
                     ARGLAWGVVTNKSMRFTGPLIREMSLFASAAAVVAGDSTPHAKPHPGPLLEAARRLSL
                     APGHCLYVGDDERDVMAGRAAGMGTVAATYGYLGGNADALCWGAHAHIGSPLELLALL
                     KSAVRA"
     gene            complement(1162..1881)
                     /gene="ubiG"
                     /locus_tag="VGA59_05470"
     CDS             complement(1162..1881)
                     /gene="ubiG"
                     /locus_tag="VGA59_05470"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX9719160.1"
                     /translation="MTANTDTAVNADPAELAKFSDLAHRWWDPESEFRPLHQINPLRL
                     DWIQSLVPLAGKRVLDVGCGGGILADAMARKGAQVLGIDLATKALKVAQLHALEAHTA
                     NVEYREISAEALASEQPGGFDAVTCMEMLEHVPDPASVVRACSTLVKPGGWVFFSTIN
                     RNPKSFLFAIVGAEYVLKILPRGTHEYTKFIRPSELAGYCRGAGLELAHARGMEYNPL
                     TRRYWMSSDTSVNYLFATQRS"
     gene            complement(1957..2634)
                     /locus_tag="VGA59_05475"
     CDS             complement(1957..2634)
                     /locus_tag="VGA59_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9719161.1"
                     /translation="MKKLNKVALLFAVAALSTAAGAQMRVTAADGGNRIDNWQNGSGE
                     IVWRNGTNELCWRNAFWTPATAAKGCDGALVPPPPPPAAAPPAARPAPPVAAPPAPAP
                     MPPAATKVTYAADAFFDFDKSVLKPEGRAKLDDLVGKVKGINLEVIIAVGHTDSVGTD
                     AYNQRLSVRRAEAVKAYLVSRGIEKNRVYTEGKGEKQPVASNRTAEGRAKNRRVEIEV
                     VGTRANR"
     gene            2864..>3516
                     /locus_tag="VGA59_05480"
     CDS             2864..>3516
                     /locus_tag="VGA59_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX9719162.1"
                     /translation="MTQFAKETLPVSLEEEMRRSYLDYAMSVIVGRALPDARDGLKPV
                     HRRVLYAMHELNNDWNRPYKKSARIVGDVIGKYHPHGDQSVYDTIVRLAQDFSMRHML
                     VDGQGNFGSVDGDNAAAMRYTEIRLAKIAHEMLADIDKETVDFGPNYDGSEKEPLVLP
                     SRLPNLLVNGSGGIAVGMATNIPPHNLNEVVDACLHLLRNPEASIDELMEIVPAPDFP
                     "
BASE COUNT          644 a         1129 c         1118 g          625 t
ORIGIN      
        1 tggcacccgc aggaccggag gccggaaccc cacaccacgc ggggatctcg ggcaagaaaa
       61 agggcccgga gactatccga gcccttgtgt atgggctggc gggaattgaa cccgcgtccg
      121 caagccttct tcgcacagtt ctacatgtgt agccgtctga ttgagtctcg cctcctgtat
      181 cgcgcagcgg cacgctatac aggacgccag caacctattg tctcgtcccg tgccaagtta
      241 cccgacacag caccagccta tgaaattatc tttgcagccg ggagccttta acttgcgcta
      301 aagacccctt gcccagccca taggcctgct gttgcaaagc tcaccggcaa ttaagcggcg
      361 agtgcgaaac gttcgtcgtt tgcagttact ttgtttgaat ctgttttacg agcacattca
      421 gctcgacatg ccctgccgcg cgtccgaacc cacgtcgaaa ccagtgcagc cccaggaagc
      481 ttgtatttta ggctcttacg gccgatttca agagggccag caattcaagc ggagagccga
      541 tgtgggcatg ggcaccccag cacagtgcgt cggcattacc gccgaggtag ccgtaggtgg
      601 cggccacggt ccccataccg gcggcccggc cggccatcac atcgcgctcg tcgtcaccga
      661 cgtaaaggca gtggccgggc gccaggctca gccggcgcgc ggcctccagc agcggccccg
      721 gatgcggttt cgcgtggggc gtgctgtcgc cggcgacgac ggcggcggcc gacgcgaaca
      781 ggctcatctc ccggatcagc ggcccggtaa agcgcattga cttgttcgtc accacacccc
      841 aggccaagcc cctggccagc agttgcgcaa tgagctcggc gacaccctcg aacgcgtggg
      901 tgcgctgggt catgcagttc tcatagttga tgaagaactc ctcgcgcaac gcggcaaaat
      961 cgggatggtc cggcgtcatg ccgaacgcca cgcccagaat gccgcgtgcg ccggcccccg
     1021 ccatcggacg gtacagctcg agcggtagtg agggcaggcc gcgcgcgacg cgcatcgtgt
     1081 cggcggctgc gcccaggtcc ggggcactgt cgatcagggt gccgtccaga tcgaacagga
     1141 ccgcctcgat atcgctgaac atcaggatct ctgcgtggca aaaagatagt tgacactggt
     1201 gtccgaggac atccagtagc gccgggtcag cgggttgtac tccatgcccc gcgcgtgcgc
     1261 cagttccagg cccgcgccgc ggcagtagcc ggcaagttcg ctcgggcgga tgaacttggt
     1321 gtactcatgc gtgccccgcg gcaggatctt gagcacatat tccgcgccga cgatggcgaa
     1381 caggaaagac ttcggattgc ggttgatggt ggaaaagaag acccagccac cgggtttgac
     1441 gagggtgctg caggcccgca ccaccgaggc cggatcgggg acgtgctcta gcatctccat
     1501 gcaggtgacg gcatcgaagc cgcccggctg ttccgacgcc agggcttcgg cactgatctc
     1561 gcggtactcg acgttggccg tgtgggcctc cagcgcatgc agctgggcca ctttcagcgc
     1621 cttggtggcc aagtcgatgc ccagtacctg tgcgcccttg cgggccatcg cgtcggcgag
     1681 gatgccgccg ccacagccca cgtccagcac gcgcttgccg gcaagcggca ccagcgactg
     1741 gatccagtcc agccgcagcg ggttgatctg gtgcagggga cggaactcgc tctccgggtc
     1801 ccaccaccgg tgggccaggt cggagaactt ggccagttcg gccggatcgg cgttgacagc
     1861 cgtgtcggta ttcgcggtca tggtagcgat tgtgccgcgc ccaaagtaaa aaggccccgc
     1921 gtcgcggggc cttttttttg cattgccgca ggggcgttag cggttggcgc gggtaccgac
     1981 cacctcgatc tcgacgcggc ggttcttggc gcgcccttcg gccgtccggt tgctggcaac
     2041 cggctgcttc tcgcccttgc cctcggtgta gacgcggttc ttttcgatgc ccctggacac
     2101 caggtaggcc ttgacggcct cggcgcggcg aaccgacagg cgctggttgt acgcatcggt
     2161 gcccacggag tcggtgtggc cgacggcgat gatcacttcg aggttaatac ctttgacctt
     2221 acccacgagg tcgtcgagct ttgccctgcc ctcgggcttg agcacggact tgtcgaagtc
     2281 gaagaaagcg tcggccgcgt acgtcacctt ggtggcggcg ggcggcatag gagccggtgc
     2341 gggaggagcg gcgacgggcg gagcaggccg cgcggcgggc ggagcagcgg ccggcggggg
     2401 cggcggcgga accagcgcgc cgtcgcagcc cttggcggcg gtggcgggcg tccagaatgc
     2461 gttgcgccag cacagttcgt tggtgccgtt cctccacacg atctcaccgg agccgttctg
     2521 ccaattgtcg atccggttgc caccgtcggc tgcggtcacg cgcatttgcg caccggcggc
     2581 cgtggacagg gcggcgacgg caaacaacag cgccactttg ttcagtttct tcatggttct
     2641 cctcttgggg aaaaagccgc agccgcgctg cgtgaatacg acgtttaagg tgaccaccac
     2701 caaaagcgtg aaatcattgt gccacacgtc cttacacaat caacggacat acggctcaag
     2761 ccacgtagga caaaacctca ttcctggcgt tctgttgctg cgctgcgaca ggcgccttga
     2821 acgtaaaatc caaggtttcg cgccgaagtt cctcttccgt ccaatgaccc aattcgccaa
     2881 agaaaccctg cctgtcagtc tcgaagagga aatgcggcgc agctacctcg attacgccat
     2941 gagcgtgatc gtgggccgtg cgttgccgga tgcgcgggac ggcctcaagc ccgtccatag
     3001 gcgcgtgttg tacgcgatgc acgagctcaa taacgactgg aaccgcccct acaagaagtc
     3061 ggcgcgcatc gtcggggacg tcatcggcaa gtaccacccc catggcgacc agtcggtcta
     3121 cgacaccatc gtgcggctgg cgcaggattt ttccatgcgc cacatgctgg tggacggcca
     3181 gggcaacttc ggttccgtcg acggcgacaa cgccgcagcc atgcgctata ccgagatccg
     3241 gttggccaag atcgcgcacg agatgctggc cgatatcgac aaggagacgg tcgatttcgg
     3301 gcccaactac gacggcagcg aaaaagagcc gctggtcctg cccagccgac tgcccaacct
     3361 gctggtcaac ggctccggcg gcatcgccgt gggcatggca accaatattc cgccgcacaa
     3421 cctcaacgag gtggtggatg cctgcctgca cctgttgcgc aacccggagg cgagcatcga
     3481 cgagctgatg gaaatcgtgc ccgcccccga cttccc
//
LOCUS       DASWIY010000144         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1567584, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000144 DASWIY010000000
VERSION     DASWIY010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567584"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VGA59_05485"
     CDS             complement(<1..527)
                     /locus_tag="VGA59_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9719163.1"
                     /translation="MKLITFCLAAILAFAAAGAAAQKKYDPGASDTEIRIGQTMPYSG
                     PASNYGEVGKTQAAYFRMVNEQGGVNGRKIKFLSLDDGYSPPKTVEGVRKLVEQEQVL
                     LLYGMFGTATNTAVHRYLNAKKVPHLLLLTGASKWNDPRKNPWTVGFYPDYPSEAKVY
                     ARHILENKPNAKIAVL"
     gene            complement(524..1480)
                     /locus_tag="VGA59_05490"
     CDS             complement(524..1480)
                     /locus_tag="VGA59_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9719164.1"
                     /translation="MNMNQRFAIAAAIAVAALLPFVLPSYQVFQFTMVLVYALALLGL
                     NLLTGYNGQISLGHGAFFALGAYTAAMLMEHAGWPYWATLPAAAVVGGVIGYLFGLPA
                     LKLDGVYLALATFALGVVTPQLLKYKGLEHWTGGSQGLVLLKPEAPFGLPLDADQWLY
                     FFCLAVALALFLVAHNLLRGSVGLSLAAVRDNPIAAGAMGVDNARVKSVTFGISALYT
                     SVAGALSAIVVQFIAPDSFTVFLSITLLVGAVVGGLSSLTGPLYGALFIQFVPHVAEQ
                     VSKAAPWAVYGAILLALMLVWPTGVAGAAAQLRGRLFKRSNS"
     gene            complement(1477..>1601)
                     /locus_tag="VGA59_05495"
     CDS             complement(1477..>1601)
                     /locus_tag="VGA59_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9719165.1"
                     /translation="LLGTYVVGAELKLSVALVLIIGVLVVRPSGLFGKAVVVRV"
BASE COUNT          294 a          535 c          523 g          249 t
ORIGIN      
        1 agcaccgcga ttttcgcatt gggcttgttt tccaggatgt ggcgtgcgta gaccttcgcc
       61 tccgacgggt agtcggggta gaagcccacg gtccacgggt tcttgcgcgg gtcgttccac
      121 ttcgatgcgc ccgtcagcag cagcaggtgc ggcaccttct tcgcgttcag gtagcgatgc
      181 acggcggtgt tggtggcggt gccgaacatg ccgtacagca gcaggacctg ttcctgctcc
      241 accagcttgc gaacgccttc cactgtcttg ggcggcgagt agccgtcgtc caggctcagg
      301 aacttgatct tgcgcccgtt cacgccgccc tgttcgttga ccatgcggaa gtaggcggcc
      361 tgcgtcttgc ccacctcgcc gtagttcgag gccggcccgc tgtagggcat cgtctggccg
      421 atacggatct cggtgtcgga ggcgccgggg tcgtacttct tctgggccgc ggcgcccgca
      481 gccgcgaatg ccaggatggc cgcaagacaa aaggtgatga gtttcatgag ttgctccttt
      541 taaaaagccg gccgcgcagc tgcgcggcgg cgccggccac gccggtcggc cacaccagca
      601 tgagagcgag caggatggcg ccatacacgg cccagggggc cgctttggac acctgctccg
      661 ccacatgcgg aacgaactgg atgaacaggg cgccgtacag cggacctgtg agggatgaca
      721 gccctcccac caccgctccc acgagcaagg tgatggacag gaagacggtg aagctgtccg
      781 gcgcaatgaa ctggaccacg atcgcgctca gggctccggc tacgctcgtg tacagcgccg
      841 aaatgccgaa cgtgacggac ttcacccgcg cgttgtcgac gcccatcgcg cccgccgcga
      901 tcgggttgtc ccgcaccgcg gcgagcgaca ggccgacgct gcctcgcagc aggttgtgtg
      961 caaccaggaa cagcgccagc gcgacggcca ggcagaagaa gtacagccac tggtcggcat
     1021 cgagcggcag gccgaagggg gcttcgggct tgagcagcac caggccctgc gagccgccgg
     1081 tccagtgctc gagccccttg tacttgagca gctgcggcgt caccacgcct aacgcgaagg
     1141 tggccagcgc gaggtacacg ccgtcgagct tcagcgcggg caggccgaac aggtagccga
     1201 tcacgcctcc cacgaccgcg gcggccggca gcgtcgccca gtagggccag cccgcgtgtt
     1261 ccatcaacat ggcggccgtg tacgcgccga gcgcgaagaa cgcgccgtgt cccagcgaga
     1321 tctggccgtt gtagcccgtg agcaggttca accccagcag cgcgagcgca tagaccagca
     1381 ccatcgtgaa ctggaacacc tggtagcttg gcagcacaaa gggcagcagg gccgcgaccg
     1441 cgatcgcggc agcaatggca aatcgctggt tcatgttcat accctcacca ccacggcctt
     1501 gccgaacagc cccgaggggc gcaccaccag cacgcctatg atcagcacca gcgccaccga
     1561 cagcttgagc tccgcgccga ccacataggt gccgagcagg t
//
LOCUS       DASWIY010000145         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1186211, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000145 DASWIY010000000
VERSION     DASWIY010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186211"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VGA59_05500"
     CDS             <1..972
                     /locus_tag="VGA59_05500"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX9719166.1"
                     /translation="LPWRKQIIGSLLQCDYIGFHIPRQVENFVDVARGVTPLQTVSRQ
                     NCAPRFITYGCAVGLERMTTAVDTGSRIVKLGAHPVGLDIGRISSALDQPKIQEQMAN
                     LRKELSGLKLILSVERLDYTKGILEKLQAFERLLADNPELLGKVTLVSICVPAAREMT
                     VYDELQAQIEQAVGRINGRFARIGWSPVQFFFRAFPFEQVVAWYAMADVMWITPLRDG
                     LNLVAKEFVAVQGLLEGHGVLALSEFAGAAAELKGALLTNPHDTADLASTCYLALNMP
                     KAEAQARLRELFDIVSYNDIRRWGDEFLAGVAEQPEAEKPATLGLAS"
     gene            complement(1186..1602)
                     /locus_tag="VGA59_05505"
     CDS             complement(1186..1602)
                     /locus_tag="VGA59_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9719167.1"
                     /translation="MKIHAYLMFDGQCEAAFNFYAQTLDGTLQLMRYAESPDAKDIPP
                     EFQQRVMHVCLTVGNQLLMASDTLPQSPYEGIKGCSISLQVDNVPEAERLYAALSAQG
                     SVQMELQQTFWATRFAMLTDRFGVSWMINCMVSEMD"
     gene            complement(1614..2039)
                     /locus_tag="VGA59_05510"
     CDS             complement(1614..2039)
                     /locus_tag="VGA59_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9719168.1"
                     /translation="MRFMVIVKATADSEAGVMPSEALLAAMGRYNEELARAGVMLAGE
                     GLHPSAKGVRVKFTGDQRSVTDGPFIETKELIAGFWIFQVASLQECIEWVKRCPNPMP
                     GESEIEIRQIFEAEDFGAEFTPELREQEARIRARIDLKD"
     gene            complement(2164..>2786)
                     /gene="fusA"
                     /locus_tag="VGA59_05515"
     CDS             complement(2164..>2786)
                     /gene="fusA"
                     /locus_tag="VGA59_05515"
                     /inference="COORDINATES: protein motif:HMM:NF015706.4"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX9719169.1"
                     /translation="RNTCEIEGKFVRQTGGHGQYGHCWIRFAPADEGQEGLQFSNEIV
                     GGVIPREYIPAIQKGIEEQMKNGVLAGYPLLGLKAAVFDGSTHDVDSSELAYKIAAST
                     ATRQLAQQGGAVLLEPLMRLEVVTPEEYMGDIMGDLNRRRGLIHGTEEAVTGRIVRAE
                     VPLGEMFGYATDVRSMSQGRASFSMEFARYAEAPAHIAAAILKKSG"
BASE COUNT          495 a          942 c          867 g          482 t
ORIGIN      
        1 ctgccgtggc gcaagcagat catcggcagc ctgctgcaat gcgactacat cggctttcac
       61 atcccgcgcc aggtggaaaa cttcgtcgac gtggcgcgcg gcgtgacgcc gctgcaaacc
      121 gtcagccgac agaactgtgc gccacgcttc atcacctatg gctgcgccgt gggcctggag
      181 cgcatgacca ccgcggtgga caccggtagc cggatcgtca aactcggcgc ccacccggtc
      241 ggcctggata tcggccggat cagcagcgcc ctggaccagc cgaagatcca ggagcagatg
      301 gccaacctgc gcaaggaact cagcgggctg aaactgattc tttcggtgga acgcctcgac
      361 tacaccaagg gtatcctgga aaaactccag gccttcgagc gcctgctggc ggacaacccg
      421 gaactgctcg gcaaggtcac cctggtcagc atctgcgtgc cggccgcacg cgagatgacc
      481 gtctacgacg aactgcaagc gcagatcgaa caagccgtgg gccgcatcaa cggccgcttc
      541 gcccgaatcg gttggtcgcc ggtacagttc ttcttccgtg ccttcccctt cgagcaggtg
      601 gtggcctggt acgccatggc cgacgtgatg tggatcactc cgctgcgcga cggtctcaac
      661 ctggtggcca aggagttcgt cgccgtccag ggcctgctcg aaggccatgg ggtgctggca
      721 ctctcggaat tcgccggcgc cgccgccgaa ctcaagggcg cgctactgac caacccgcac
      781 gacaccgcgg acctggccag cacctgctac ctggcgctga acatgcccaa ggccgaggcc
      841 caggcgcggc tgcgcgagct gttcgacatc gtcagctaca acgacatccg ccgctggggc
      901 gacgagttcc tcgccggggt ggccgaacag cctgaagccg aaaagcctgc aaccctgggt
      961 ttggccagct aagggttacg tcctggtgcg cggggccgcc taggtcgcgc gcaccaggca
     1021 gccgaaacgg gctcaccctg cctggttgtc catcctaccc ggctcaacgc cgggcttgcg
     1081 tgggagggcg ttcgcggtca agacccaccc cggccctgcc cgcgacttga ctgccgcagg
     1141 gggcgtcggg cgcaacgaac tccagcaggg cgcgcgccgc ctggctcagt ccatctcgct
     1201 taccatgcag ttgatcatcc aggacacacc gaagcggtcg gtgagcatgg cgaagcgcgt
     1261 ggcccagaag gtctgttgca gttccatctg caccgagccc tgggcagaca acgcggcgta
     1321 caggcgctcg gcttcgggca cgttgtccac ctgcagcgag atcgaacagc ccttgatacc
     1381 ctcgtacgga gactgcggca gggtgtcgga ggccatcagc aactggttgc ccaccgtcag
     1441 gcagacatgc atgacacgct gctggaactc cggcggaata tccttggcat cgggactctc
     1501 ggcataacgc atcagctgca gggtgccgtc cagggtctgg gcatagaagt tgaaggcggc
     1561 ctcgcactgg ccgtcgaaca tcaggtaggc atggatcttc atctgcattc tcctcagtct
     1621 ttaaggtcga tgcgcgcgcg aatacgggct tcctgctcgc gcaactcggg ggtgaattcg
     1681 gcgccaaagt cttcggcttc gaatatctga cggatctcga tctccgactc gccaggcatc
     1741 gggttggggc agcgcttgac ccactcgatg cactcttgca atgaagcgac ctggaagatc
     1801 cagaaaccgg cgatcaattc cttggtctcg atgaaagggc cgtcggtcac gctgcgctgg
     1861 tcgccggtga acttcacccg cacgcccttg gcgctggggt gcaggccctc gccggcgagc
     1921 atcacgccgg cgcgggccag ctcctcgttg tagcgaccca tggccgccag cagcgcctcg
     1981 ctgggcatca ccccggcttc ggaatccgcg gttgccttga ctatcaccat aaaacgcatc
     2041 gtcgtctctc ctcgcagcta cacaaccacg cggcaagtgg cgtggcgtct gcccagtagt
     2101 cgaacgcgcc agacgcgaat cgacagcgca cgccgacttt tcatcccgcc ctaaccgttg
     2161 ccttcaaccg gacttcttga ggatggcggc ggcaatatgc gccggcgcct cggcgtagcg
     2221 ggcgaactcc atggagaagc tggcgcggcc ctgggacatc gaacgcacgt cggtagcgta
     2281 gccgaacatc tcgcccagcg gcacttcggc gcgcacgatg cgccccgtca cggcttcctc
     2341 ggtaccgtgg atcaggccgc ggcggcgatt gaggtcgccc atgatgtcgc ccatatattc
     2401 ctcgggcgtg accacctcga ggcgcatcag cggctccagc agcaccgccc cgccctgctg
     2461 cgccaactgc cgggtggccg tcgaggcggc aatcttgtag gccagttcgc tggaatcgac
     2521 gtcatgggtg gagccgtcga acaccgcggc cttgagcccg agcaacggat aaccggcgag
     2581 cacgccgttc ttcatctgtt cctcgatgcc tttctggatc gccggaatgt attcgcgcgg
     2641 gatcaccccg ccgacgatct cgttgctgaa ctgcagcccc tcctggccct cgtcggccgg
     2701 cgcgaagcgg atccagcagt ggccatactg gccgtgcccg ccggtctggc ggacgaactt
     2761 gccctcgatc tcgcaggtgt tgcgga
//
LOCUS       DASWIY010000146         2298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2676358, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000146 DASWIY010000000
VERSION     DASWIY010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2676358"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VGA59_05520"
     CDS             complement(<1..406)
                     /locus_tag="VGA59_05520"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HEX9719170.1"
                     /translation="MAERAQKLSLFDPAIVRRAIRDSVIKLHPRTLMRNPVMFVVEVV
                     AAVTSILFIRDLFGGTESSAFVGQIAAWLWFTVLFANFAEAIAEGRGKAQADALRRTK
                     TQTMAKRLRDPASRDYELVPATDLQRGDLVIVE"
     gene            complement(419..2149)
                     /gene="kdpA"
                     /locus_tag="VGA59_05525"
     CDS             complement(419..2149)
                     /gene="kdpA"
                     /locus_tag="VGA59_05525"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX9719171.1"
                     /translation="MTANGLLQIALYVGVLVLCIKPLGLYMARVFNGERTCLDPVMRP
                     VERLIYKVSGVDPRQEQHWTTYTAAMLLFSLASFVLLYALQRLQHVLPLNPQGFDPPS
                     PDSAFNTAVSFTSNTNWQSYAGESTMSYLSQMAGLTVQNFLSAAVGIVLAIALTRGFA
                     RRSAQTVGNFWVDLVRTTLRILLPLSFIFALVLVWQGVPQNLQKYVDATTVEGGSQTI
                     AQGPVASQEAIKMLGTNGGGFFNANSAHPYENPTPLSGFLEMLAILMIGAALTYTFGH
                     MVGDTRQGWALFGAMMAVFLMGLAVAYWAESAGNPAFDRLGIQTAASAAQAGGNMEGK
                     EVRFGISNSALWATATTAASNGSVNSMHDSYTPLGGLVPLFNIQLGEVIVGGVGSGLY
                     GMLLFAIVAVFIAGLMVGRTPEYLGKKIEAKEVKMSVLALVLILPLGILGFTALASVH
                     PEGLKGPLNAGPHGFSEILYVYTSGTGNNGSAFAGISANTFFYNLTNGLSMLVGRFLF
                     IVPMMAVAGSLVNKKIVPASAGTFPTASGLFVGLLVAVILIVGGLVYFPALALGPIVE
                     HLLMMTGKLF"
     gene            complement(2162..2251)
                     /locus_tag="VGA59_05530"
     CDS             complement(2162..2251)
                     /locus_tag="VGA59_05530"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF011342.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEX9719172.1"
                     /translation="MILDYLLGGLVTLGLLVYLVYALLRPERF"
BASE COUNT          487 a          732 c          681 g          398 t
ORIGIN      
        1 cctcaacgat gaccagatcc ccgcgctgca gatcggtcgc cgggaccagt tcataatcac
       61 gactcgcggg atcgcgcagc cgcttggcca tagtctgcgt cttggtgcgc cgcagggcat
      121 ccgcctgggc cttgccgcgt ccttcggcta tggcttctgc aaagttggcg aagaggacag
      181 tgaaccacag ccacgcggcg atctgcccga cgaaggccga gctttccgtg ccaccgaaga
      241 ggtcacggat gaagagaatg cttgtcactg cggcgacgac ctcgaccacg aacatgaccg
      301 gattgcgcat cagcgtgcgt ggatggagct tgatcactga atcccgaatc gcccgccgca
      361 cgatcgcagg atcgaacagc gagagttttt gcgcgcgctc agccatggga ccctccccct
      421 aaaataattt tccagtcatc atgagcaggt gctcgacgat cggccccagc gccaaggctg
      481 ggaaatagac taacccgccc acaatcaaga tgactgcgac tagcaggccg acgaacaggc
      541 cgctggcggt cgggaacgtg ccggccgagg ctgggacgat tttcttgttg accagcgagc
      601 cggccacggc catcatcggt acgatgaaca ggaagcgtcc cacgagcatg gagagaccgt
      661 tcgtcaggtt gtagaagaaa gtattggcgc tgatccccgc aaaggcgctg ccgttattcc
      721 ccgtgcccga ggtatagaca tacagaatct cactgaagcc atgtgggccg gcgtttaatg
      781 gcccctttag tccttccgga tggacgctgg ccagcgcggt aaagccgagg atgccgagcg
      841 gcaggatgag cacaagggcc agtacgctca tcttgacttc cttcgcctcg atcttcttcc
      901 cgagatactc cggcgtccgc cccaccatca gcccggcgat aaacaccgcc acgatggcaa
      961 ataacagcat gccgtacagg ccggagccca ccccaccgac gatcacctcg ccgagctgga
     1021 tgttgaagag cggcaccagc cccccgagcg gcgtatagga gtcgtgcatg gagttgaccg
     1081 agccgttcga cgccgcggtg gtcgccgttg cccacagcgc cgagttggag atgccgaagc
     1141 gcacctcttt gccctccatg ttgccgccag cctgcgccgc actggcggct gtctggatcc
     1201 ccaggcggtc gaaggcggga ttgccggccg actcggccca gtaggctacg gccaggccca
     1261 tcaagaacac cgccatcatt gccccgaaga gggcccagcc ctggcgggta tcgccgacca
     1321 tatggccaaa cgtataggtc agcgccgccc caatcatcaa gatcgccaac atttctaaga
     1381 agcccgacag cggcgtaggg ttctcataag ggtgggccga gttggcgttg aaaaaaccgc
     1441 cgccattggt gccgagcatc ttgatcgcct cttgtgaggc cactggaccc tgcgcgatcg
     1501 tctgcgagcc accttccact gtggtcgcgt caacatattt ctgcagattt tgcggcactc
     1561 cttgccagac taatacgagg gcgaaaataa acgacagcgg cagcaagatc cgcagcgtgg
     1621 tgcgcaccag gtcgacccag aagttgccca cggtctgcgc cgagcggcgg gcgaagccgc
     1681 gtgtcagcgc gatggccagg acgatgccca ccgccgccga cagaaagttc tgcaccgtca
     1741 agccggccat ctgcgacaga tagctcatcg tgctttcgcc cgcgtaggac tgccaattag
     1801 tgttgctggt gaaactcacg gctgtgttga aggccgagtc tggcgaaggg gggtcaaagc
     1861 cctgcgggtt cagcggtagc acatgctgca gccgttgcag ggcatagagg agcacgaagc
     1921 tggccagact gaacagcagc atcgccgccg tgtacgtcgt ccaatgctgc tcttgccgtg
     1981 gatccacccc actgaccttg taaatgaggc gctcgaccgg acgcatgacc ggatcgaggc
     2041 acgtccgctc gccgttgaac acgcgcgcca tgtacagccc caggggcttg atgcacagca
     2101 cgaggacccc gacgtacagg gcgatttgca gtaaaccgtt cgccgtcatc gcactttccc
     2161 cctaaaatcg ctcgggtcgc aacaaggcat agacgagata gaccagcaag ccgagtgtga
     2221 ccaggccgcc cagcagataa tccaggatca tgcgtttccc tccgccatgg gttgccgtta
     2281 gagccgctca cagcccac
//
LOCUS       DASWIY010000147        19071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_455122, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000147 DASWIY010000000
VERSION     DASWIY010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 19071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19071
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455122"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /locus_tag="VGA59_05535"
     CDS             complement(<1..1727)
                     /locus_tag="VGA59_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9719173.1"
                     /translation="MPSYTPPLRDMRFVMHEVLKVTDEFKAIPKYVEADAETIDAVLE
                     EAGKFATEVTFPLNATGDVEGCKLDPATHEVETPTGFKEAYAQYVEAGWPALSCDPEW
                     GGQGLPLVVNQCFYEMLNSANQAWTMYPGLTHGAYAALATHGTDEQKQTYLPKMTSGE
                     WTGTMCLTEAHCGTDLGLMRTKAEPQPDGSYRITGNKIFISAGEHDMAQNIIHLVLAR
                     LPDAPPGIKGVSLFIVPKFLVNKDGSLGERNSIFCGGLEHKMGIHGNSTAQIVIDGAS
                     GWIVGQPNKGMQAMFVMMNAARLGVGNQSLGLTEVAYQNALAYAKERIQMRSLTGPKA
                     KDKPADPIIVHPDVRKMLLTAKAFAEGGRALAIYCAVLLDKAHYHPDEKVRKDSDEMV
                     SLLTPIVKAFTTDNGHIATNACLQVFGGHGYIHEWGMEQFARDNRINMIYEGTNTIQS
                     LDLLGRKILGNNGATLKKFGKLVGQLVEEEGVNEKMAEFINPIAYLGDQMTKFTTELG
                     FKGFQNADEVGAAAVDYLRVLGHLVHGYLWARMAQVALREIAAGNTDPFYQAKLQTAR
                     FYFAKLFPET"
     gene            complement(1730..2431)
                     /locus_tag="VGA59_05540"
     CDS             complement(1730..2431)
                     /locus_tag="VGA59_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9719174.1"
                     /translation="MSVTELPLKPQRPPRAARPHQKGLQTKAAIVDAALGLATQIGLE
                     GLSIGALAEVTQMSKSGVFAHFGSREELQISVIREYHTRFEDEVFYPAMQEPRGLPRL
                     RALFGNWMKRTSIEIDSGCIYISGAVEFDDRVGPVRDALVGSVKTWLAAMRRAVMSAK
                     EEGHLRADADEDQLLFEIHGLILALHYEARFLKNPGSIDRANAGFDNILKLYGADALP
                     ARSFKSNRSTKSPKE"
     gene            complement(2606..3229)
                     /locus_tag="VGA59_05545"
     CDS             complement(2606..3229)
                     /locus_tag="VGA59_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9719175.1"
                     /translation="MQRHSYEDGARLLISPLALPDLTPHQALRVVPYMTLTLAHRGAV
                     LFRAGGPGSHFLVMLLDGDAVVEGQLTGASEPIVLRTLVAGSMFGELGAEDSAGRWVT
                     VRATSETCLATLDYPALDALTAADPTLGCALLRAILAHAMRRLRSATTKIETLNEINN
                     ALKEEWSAEVKSDKDTIARLSVLMKLERQAGLRSRLGDDRVGIKKRA"
     gene            complement(3245..3757)
                     /locus_tag="VGA59_05550"
     CDS             complement(3245..3757)
                     /locus_tag="VGA59_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX9719176.1"
                     /translation="MEQQLLQSIDLPAVLPEAAADEPRAPARLLEFKRGSFVAFPAHA
                     TIGLIDNPTVTPVPGAPYYCLGLMPWQGRQLPLLDLNTLLRAYPEGDAPPAGHVLVLA
                     YQSAPGRPLEYGAVCAPWLVRMIEVGDSQQCELPCDSDLWPWISLACFDYDGHAVPVL
                     DTSRVFGPPQ"
     gene            complement(3762..4193)
                     /locus_tag="VGA59_05555"
     CDS             complement(3762..4193)
                     /locus_tag="VGA59_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719177.1"
                     /translation="MNMSGNFVLLRADSLRLLLPQEDVGAAEYVEHELRPSGEAGVYE
                     HGEGDNLRWVVALSAQMRPLPGFPADRFVLASLRTGESALSFAWNEVQVLIDAQFERH
                     ELPVAMQVPGAPIEAYVERDGQVVLCTNAESVVSYALGAAG"
     gene            complement(4190..7018)
                     /locus_tag="VGA59_05560"
     CDS             complement(4190..7018)
                     /locus_tag="VGA59_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719178.1"
                     /translation="MKLNDLIEALAAEVEMAQPRLVESLEELAGLDAEDPGFMDALEQ
                     YGGQAQRMGEAAELAGFPGLQAVCNHVLENTFAMPMLAPAERGPLVEFLRGWPPLIVY
                     YLRRMGEPTAAAGLVDHLVKAPYPLDEEQSLKVMHMLGAMPQQMQPHGGAGGEEMRPV
                     LATPDDVALVMPQDVDQKLLEGFFQEAPDHARYLVDLARNMAAGQGDHSDIVAAKRVA
                     HTLKGSGAIIGLRGLASLGHHFEDILEHFEREGGHVAKPAADALLDAAYCLEQMVGYV
                     AGSDEYPQQAQAVLQSVLDLANRIDRGESLESPVSRVPAAVADAAARESEPDRAPAAA
                     ATAAPTAALRVSMERVDELFRVSGEVSVHSAAMEARIKALAERSRELLAQNLRVQKRL
                     FELETLVDVRALTMMRARTRGADEEAFDPLEMDQYSELHNTAHALIEEAADARVLALR
                     LEDGIAGLGSVQTRQDRLAKDLQHLVIGTRMTAAAVLESRLQRNVRSTCQATGKQAVL
                     VLKGGDTQIDSDVLNRLADPLLHLLRNAVDHGVEPPAERVAAGKDETGHIELAFSRQG
                     QQVVLRCSDDGRGLNLPAIRRRAAERGLADADAPLADDEVSRLVLLPGFSTRDCVSEV
                     SGRGVGLDVVRDWAATMGGSVRIQSRIGQGCIVELRFAASLSTIQSLIVEAGGQRFAL
                     PSLNIEQAVPRGAGSFEHLGEQLVYRYGKRVLPALHLAEAAGLPLAADKPLDQYDAVI
                     LRLDHKTQALAVDRLIDSRELLVKSPGLYARHVRGVVGLSILGDGSVAVNLDLSQLVA
                     SGGAQRHARQATPRVAQPTPKRNLPGVLIVDDALSVRNTLVQLVQDAGFRAEAARDGI
                     DAIDTLRVFKPDLVLTDLEMPNMNGIELTTHIRGRDDLKGLPVIMITSRSQEKHRKLA
                     RQAGVDTYITKPYNDGDLLQTIREALAV"
     gene            complement(7052..8851)
                     /locus_tag="VGA59_05565"
     CDS             complement(7052..8851)
                     /locus_tag="VGA59_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9719179.1"
                     /translation="MKLDLNLKIRTKLLIAFGGLLVLAGLSARLAAWEMGELTSLQKQ
                     VQGKTAMLARANNAMWELRFHLAQYVLYPDAANRQRINEAGPKQLAIVEENFKAFAAM
                     EGLTPEEVQGHKRMLEGYAKYKEKRPVWFDLVDKGKLEEASVFRATETNPAAAETVKG
                     LNDLLETTVKSDVVKREELKKTGEIFLLLAAITMVLATGASAFFLYRDVNRPLARLHD
                     TIEKVSAGDYEARAGLTTDDELGEFAKNFDRLLDERIAAQRAAEDENMRLNNSVIAIL
                     QSVNLLSQRDLTVRAPVTEDVMGTVGDSINALTDETTKVLHGVNRIAGQVAQASGKVQ
                     AQAALVSRTAADERTNVAQMIESLADATQTMNQISALAEQSNASAEQATQATDTALDT
                     VNGTVRGMESIREMIAETEKRIKRLGERSQEISGIVNLINTISERTHVLALNASMQAA
                     VAGEAGRGFAVVAEEVQRLAESSRTATQQIATLVNNIQLETNETIGTVNRTIGQVVQG
                     SEQAQKAGEQMRRTQEITGQLVAQVKRIGDASDLQKSMSANLLGSVQRMGESTERTSQ
                     QIDAQNVDTESLLQSANRLVESVNVFKLPLAAA"
     gene            complement(8912..9502)
                     /locus_tag="VGA59_05570"
     CDS             complement(8912..9502)
                     /locus_tag="VGA59_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX9719180.1"
                     /translation="MQAGGGLHGAAAPAPLLAPTEALLRGFVFDTPAAVPEAVPVRAA
                     GDAPGAILLREGFRIGELRLMIRYQDGNELTDLPQVYLLPRAPAWFRGMANLHGTLVP
                     VFDPAELFGVAHDEKAKPMLLVLGHGDEKAGVVIDGLPQRLRLTESDRIEDAAMPPSL
                     AGCVNHAYWSEGLDWMDLQVDHLLRTLAEELAGTAQ"
     gene            complement(9514..9870)
                     /locus_tag="VGA59_05575"
     CDS             complement(9514..9870)
                     /locus_tag="VGA59_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719181.1"
                     /translation="MFRRVLIIDDALADRTNLERIVSDAGHTPLLADSGAQGIERAKR
                     DKPDLILMDVNMPDMDGFAATRKLKADDATKHIPVVFVTGKNQRAEMAWGQMLGARGY
                     VTKPYSREQIVAELNA"
     gene            complement(9928..10755)
                     /locus_tag="VGA59_05580"
     CDS             complement(9928..10755)
                     /locus_tag="VGA59_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719182.1"
                     /translation="MVKGLKPIERQLLEGLVKVSQRRTPRLEILPGKQATDADVIVID
                     TRDAAAVAWTKRHPWIERRAVIWIDGTEAPAGHTLLRRPVQWPILPMVLARALESGPG
                     TAAAAPLAGHLAAAPARRPVRDPQILVVDDSLAVRAHLRSLLEPRGFVVTDADSVEVA
                     LQSVARRSFDCVLMDVLMPEVDGYEGCRQIKSRLRGANAIPVVMLTSKGSPFDRIRGK
                     MAGCDAYLTKPVDPEHLGEVLAQQLSAMPERREPPAAPRTESSIPRITARFAATRPV"
     gene            11134..11487
                     /locus_tag="VGA59_05585"
     CDS             11134..11487
                     /locus_tag="VGA59_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719183.1"
                     /translation="MVEPNAAAPSRHNDMVQIFYSWGLPGLLAYLSFWVALLKRVYSR
                     FWVNGEYWPACALVALFPSMVTDVGFQHYEKAAFLVLLCAFCIAWPEKKAAAAGPALA
                     PRAADTAPCDAPLKP"
     gene            complement(11477..12325)
                     /locus_tag="VGA59_05590"
     CDS             complement(11477..12325)
                     /locus_tag="VGA59_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9719184.1"
                     /translation="MKLVRYGNPGKEKPGLIDLEGRLRDLSPVVPDIGPAQLGTVGLA
                     RIRKANPQKLPLVRGTPRFGCPVSQVGKFIAIGLNYADHAAESGVAIPKEPVVFMKAT
                     SCIQGPNDAVMLPRASVKTDWEVELGVVIGTRARYVPTKDALACVAGYCVVNDVSERE
                     YQLERGPQWDKGKGCDTFGPIGPWLVTTDEIVNVQRLDMWLDVNGKRMQTGNTRTMIF
                     NVAKLVSYVSHFMTLLPGDVITTGTPPGVGLGMKPPTYLRKGDVITLGIEGLGEQRQL
                     VVPFKA"
     gene            complement(12660..13406)
                     /locus_tag="VGA59_05595"
     CDS             complement(12660..13406)
                     /locus_tag="VGA59_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9719185.1"
                     /translation="MNRLDFEGRHAVVTGGAAGLGYAIAARLIASGGGVTLWDRDFDA
                     AQRAAGVLGANAQAVEVDVARHESVQRAVQSTLRHTRRVDALINSAGITGPNIKVWDY
                     PVGAWRQVIEVNLNGLFHCCREIVPLMRDAGYGRVVNIASVAGKDGNPNASAYSASKA
                     AVIALTKSLGKELADTGVRVNCVTPAAVKTAIFDQMSEQHIQFMLSKIPMGRFGTPEE
                     VAALVAWLCTEECSFSTGAVFDLSGGRSTY"
     gene            complement(13403..14137)
                     /locus_tag="VGA59_05600"
     CDS             complement(13403..14137)
                     /locus_tag="VGA59_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9719186.1"
                     /translation="MRLEGKTALVTAAGQGIGRASALALAAEGAQVWATDVDANLLAS
                     YEGIAGVRTAALDVLDKGAIERLISGLPALDVLFNCAGFVHSGTALQATDEEWNFALN
                     LNVRSQFWAIQAALPKMVAAGRGSIINMASVCSSIKGLPNRFIYGTTKAAVIGLTKSV
                     AADFVADGIRCNAICPGTVDTPSLQDRINAYEDPEAARKNFIARQPMGRLAQAHEIAP
                     LVVFLASDESTFVTGQAYAVDGGITI"
     gene            14401..15126
                     /locus_tag="VGA59_05605"
     CDS             14401..15126
                     /locus_tag="VGA59_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX9719187.1"
                     /translation="MPLQTVEPQRLYRQIAEQLRSLVATGEFSPGSRLPAERDLARQL
                     GVSRPSVREALIALEVEGVIDVRTGSGIYVRATYTANGAGTARPQEHDPGEWGPLELM
                     RARELVEGEVAALAARNARRAQIAAIAGAMAQMREEAAAGIIPRAGDEAFHTAVAQAC
                     GNEVLHDTIRSYWRARSGPLFERLGDYFENPASWNAALVEHTAVLEAIRGHDSAAARL
                     AMQAHLKKACSRYSASWRRANSS"
     gene            15159..16148
                     /locus_tag="VGA59_05610"
     CDS             15159..16148
                     /locus_tag="VGA59_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HEX9719188.1"
                     /translation="MTKRHTLKLIAACALLATAAAPFGLAQAQTKLKWAHVYETSEPY
                     HKWSVWAGEEFKKRTNGKYEITVFPASSLGKESDINQGLQLGTVDIILTGASFAARTF
                     PRLAVSYYPFTFRDADHVLKYAKSDVFKELAEGYKKASGGNTITAMTYYGVRHSTSNK
                     AFKNCDEMKGLKMRVPDAPAYTAMPRACGANPTPIAFAEVYLALQNGTVDAQENPLPT
                     IEAKKFYEVQKNIILTGHIADALLTIVSPGLMSKLTPAEQKILVDLTQEAAEKASNDI
                     RKREVELVDEFKKKGINVITVDRNDFQKRVLKAIPFESMGLDKKDWDRVIAIK"
     gene            16186..16725
                     /locus_tag="VGA59_05615"
     CDS             16186..16725
                     /locus_tag="VGA59_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9719189.1"
                     /translation="MGADGEFHVSDEAVDLSGTTVEAWLALGFFWVLGATVVYQFVTR
                     YVMNDSAAWTEEIARYLLIATVFIGATIGVVKNNHIQVDFLYRYMPRGMARFMATAVD
                     IMRIAFFAAAAVLTVMMMLKLGSNSRMTLVDLPMNWVYGMCLLGFAAMTLRSIQVAWI
                     HRKRGYSVLERPESHMTDR"
     gene            16767..18161
                     /locus_tag="VGA59_05620"
     CDS             16767..18161
                     /locus_tag="VGA59_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9719190.1"
                     /translation="MLKFIFLLFMGSGLPVAIAMAGSVLVYILWTGNLPAFVVIHRMV
                     GGIDSFPLLAVPFFILAGNLMNNAGITNRIYNYALALVGWLKGGLGHVNVIGSVVFAG
                     MSGTAIADAAGIGTIEIKAMKDHGYSSEFAVGVTAASATLGPIIPPSLPFVIYGMMAN
                     VSVGALFLAGILPGVLMALLMMLTVAYYAHKNGWGSDIKFEWPRFTKAIAETLVVIGW
                     PVALWLLIVKVGLPPQATVGAGLVILFAADKIFSFQAVLPIMTPVLLIGGMTTGIFTP
                     TEGAIAACLWSMVLGFGWYGTLSWKKFVKICLDTVETTATVLFIVAAASIFGWMLTAT
                     GVTADIAAWVLGFTKEPWVFLLLANLLMLFVGCFLEPTAAITILVPILVPICQQLGID
                     LVHFGLVMVLNLMIGLLHPPMGMVLFVLARVAKLSVERTTMAILPWLVPLLGSLAVLT
                     YFPKLVMWLPKMFY"
     gene            18217..>19071
                     /locus_tag="VGA59_05625"
     CDS             18217..>19071
                     /locus_tag="VGA59_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX9719191.1"
                     /translation="MTPFIRLHPQDDVLIARTQLVGGATAENVVIKGLIPAGHKVATR
                     AIAQGEPVRRYNQIIGFASKPIAPGEHVHTHNLAMGDFERDYAFGADVKPAAPRREAT
                     FMGIKRADGRVATRNFIGILSSVNCSATAARAIADYFSRQNHADALAAFPNVDGVVAL
                     THGTGCGMATEGLGMQILERTLAGYATHANFWGVLVVGLGCEANQINAWLAHSSLREG
                     ETLKVFNIQDTGGTRKTVEKGIALIREMLPQANAVKREPCSASHITIGLQCGGSDGYS
                     GISANPALG"
BASE COUNT         3035 a         6397 c         6191 g         3448 t
ORIGIN      
        1 gtttccggga acagcttggc gaagtagaaa cgcgccgtct gcagcttggc ctggtagaac
       61 ggatcggtat tgccggccgc gatctcgcgc aacgcgacct gcgccatgcg cgcccacaaa
      121 tagccgtgca ccaggtggcc cagcactcgc agatagtcga cggctgcggc gccgacttca
      181 tcggcgttct gaaacccctt gaagccgagc tcggtcgtga acttggtcat ctggtcgccc
      241 aggtaggcga tcgggttgat gaactccgcc atcttctcgt tcacgccctc ctcttcgacc
      301 agttgcccca ccagcttgcc gaacttcttc agcgtggcgc cgttgttgcc gaggatcttg
      361 cgccccagca ggtccagcga ctggatggtg ttggtgccct cgtaaatcat gttgatgcgg
      421 ttgtcgcgcg cgaactgctc catgccccat tcgtggatgt agccgtggcc gccgaagacc
      481 tgcaggcagg cgttggtcgc gatgtgcccg ttgtcggtgg tgaaggcctt cacgatcggc
      541 gtgagcagcg acaccatttc gtcgctgtcc ttgcgcacct tctcgtccgg gtggtagtgc
      601 gctttgtcca gcagtaccgc gcagtagatc gcgagcgccc gcccgccctc ggcaaacgcc
      661 ttggcggtca gcagcatctt gcgcacgtcg gggtggacga tgatcgggtc ggccggtttg
      721 tccttggcct tggggccggt cagcgaacgc atctggatgc gttccttggc atacgccagc
      781 gcgttctggt aggcgacctc cgtcaggccc agcgactggt tgcccacccc gagtcgtgcg
      841 gcgttcatca tcacgaacat ggcctgcatg cccttgttcg gttgtcccac gatccagccg
      901 gatgcgccgt cgatcacgat ctgggccgtg gaattgccgt ggatgcccat cttgtgctcc
      961 aggccgccgc agaagatgga attgcgttcg cccaggctgc catccttgtt caccaggaac
     1021 ttcgggacga tgaacaggct gacgcccttg atgccgggag gcgcgtcggg caggcgggcc
     1081 agcaccaggt ggatgatgtt ctgcgccatg tcgtgctcgc cggcactgat gaagatcttg
     1141 ttgccggtga tgcggtagct gccgtcgggc tgcggctcgg ccttggttcg catcaggccg
     1201 aggtccgtgc cgcagtgcgc ctcggtcagg cacatggtgc cggtccactc gccgctcgtc
     1261 atcttcggca ggtacgtctg cttctgctcg tcggtgccgt gggttgccag cgccgcgtag
     1321 gcgccgtgcg tcaggcccgg gtacatggtc caggcctggt tcgcgctatt gagcatttcg
     1381 tagaagcact ggttcacgac cagcggcagg ccctgcccac cccactcggg gtcgcagctc
     1441 agggccggcc agccggcctc cacatactgc gcgtaggctt ccttgaaacc cgtgggagtc
     1501 tccacctcgt gggtcgccgg gtcgagcttg cagccctcga cgtcgcccgt cgcgttcaga
     1561 gggaaagtga cctcggtcgc gaacttgccc gcctcttcca gcaccgcgtc gatggtctcg
     1621 gcgtcggcct cgacatattt cgggatggcc ttgaattcat ccgtgacctt cagcacttcg
     1681 tgcatcacga atcgcatgtc gcgcaggggc ggggtatagc tgggcatggt tactccttcg
     1741 ggcttttggt ggatctgttt gatttgaatg agcgggccgg caaggcgtcg gctccgtaca
     1801 gcttgagaat gttgtcgaac ccggcgttgg cacggtcgat cgagccgggg ttcttgagga
     1861 aacgcgcttc gtaatgcagc gccaggatca ggccgtggat ctcgaacagc agctggtctt
     1921 cgtcggcgtc ggcgcgcagg tggccctctt ccttggccga catcaccgcg cggcgcatgg
     1981 cagccagcca ggtcttcacc gaaccgacca gtgcatcgcg caccgggccg acgcggtcgt
     2041 cgaactccac ggcgccgctg atgtagatgc agccggaatc gatctcgatc gaggtgcgct
     2101 tcatccagtt gccaaacagc gcccgcaggc gcggcaggcc gcgcggctcc tgcatggccg
     2161 ggtagaacac ctcgtcctcg aaacgcgtgt ggtattcgcg gatgacggag atctgcaatt
     2221 cctcgcgcga gccgaaatgg gcgaacacgc cggacttgct catctgcgtg acctcggcca
     2281 gggcgccgat ggacaggcct tccagcccga tctgggtggc caggccgagg gcggcatcca
     2341 cgatggcggc cttggtctgc aggcccttct ggtgaggccg ggccgcgcgc ggcgggcgct
     2401 gcggcttcag gggcaattcg gtgacggaca tggggcttgc gcaataaaac gaacgatcgt
     2461 tctattttgc acgaaagccc gggacgtcaa tgggttttag tcggatctag atgcttgttt
     2521 ctagcggctt tgcgggttaa ccctttgaac ccggctcaat atgaaatctt aagtccccaa
     2581 cagtggttgc ggagaacccc ggcagctacg cccgcttttt gatgcctacg cggtcatccc
     2641 cgaggcggct gcgcaggccc gcctggcgtt cgagcttcat gagcacactc agccgggcga
     2701 tggtgtcctt gtccgacttg acttcggcgc tccattcttc cttcagcgcg ttgttgatct
     2761 cgttgagcgt ctcgatcttc gtcgtcgccg agcgcaggcg cctcattgcg tgcgcgagga
     2821 tggcgcgcag cagcgcgcaa cccagcgtcg ggtctgcggc tgtcagggcg tcgagcgccg
     2881 gatagtccaa ggttgcgagg caagtttccg acgtggcccg caccgtcacc caacgtcccg
     2941 cgctgtcctc ggcccccagc tcgccgaaca tgctgcccgc gaccagcgtc cgcagcacga
     3001 tgggctcgct ggcgccggtc agctggcctt ccaccaccgc gtcgccgtcc agcagcatga
     3061 ccaggaagtg gcttcccggg ccgcccgcgc gaaacagcac ggcgccgcga tgggcgagcg
     3121 tgagcgtcat gtacggcacg actctcagtg cctggtgggg cgtgaggtcg ggcagagcca
     3181 ggggtgaaat cagcagccgc gcgccgtcct catacgaatg ccgttgcatt gttccttcct
     3241 ctatctattg cggcggcccg aagacgcggc tggtgtccag caccggcacc gcgtggccgt
     3301 cgtagtcgaa acacgccagt gagatccacg gccacagatc actgtcgcag ggcagctcgc
     3361 attgctggct gtcgcccacc tcgatcatcc gcaccagcca gggtgcgcac accgcgccgt
     3421 attccagggg tctaccgggt gcgctttgat acgccagcac cagcacgtgc ccagccggtg
     3481 gcgcatcccc ttccggatag gcgcgcaaga gcgtgttcag gtccagcagc ggcagctggc
     3541 ggccttgcca aggcatcagg ccgaggcagt aatacggcgc ccccggtacc ggcgtcaccg
     3601 ttggattgtc gatcaaaccg atggtggcat gggctggaaa tgcgacaaag gagccccgct
     3661 tgaactccag cagtcgcgcc ggggcgcgcg gctcgtcggc ggcggcttcg ggcaggacgg
     3721 ccgggagatc gatggattgg agcaactgct gttccatggt gtcagcctgc ggcgccgagg
     3781 gcataggaaa ccacgctctc ggcattcgtg cacagcacca cctggccgtc gcgctcgacg
     3841 taagcttcga tcggcgcccc cggcacctgc atggccactg gcagctcatg gcgctcgaat
     3901 tgcgcgtcga tcagcacctg gacctcgttc caggcgaatg agagcgcgct ctcgcccgtg
     3961 cgcaggctcg cgagaacgaa ccggtccgcc gggaaaccgg gcagcggacg catctgggcg
     4021 gagagcgcga cgacccaccg caggttgtcg ccttcaccat gttcatacac acccgcctcg
     4081 ccgctgggac ggagctcgtg ttcgacgtat tcggctgcgc ccacatcctc ctggggcagc
     4141 agcagccgca gcgaatccgc gcgcaacagg acgaagttgc cgctcatgtt catacggcca
     4201 atgcctcccg tatggtctgc aacaggtccc cgtcgttgta aggcttggtg atgtaggtat
     4261 cgacgccggc ctgccgcgcg agcttgcggt gcttttcctg cgaacgcgag gtgatcatga
     4321 tcacgggcag gcccttcagg tcatcgcggc cgcggatgtg cgtcgtcagc tcgatgccgt
     4381 tcatgttggg catctcgagg tccgtcagga ccaggtccgg cttgaagacc cgcagggtat
     4441 cgatcgcatc gatgccgtcg cgtgcggcct cggcgcgaaa gccggcgtcc tgcaccagct
     4501 gtaccagcgt gttgcgcacg gacaaagcgt cgtcgacgat cagcacaccg ggcaggttcc
     4561 ttttcggcgt gggctgcgca acccgcgggg tggcctggcg cgcatggcgc tgcgcgccgc
     4621 cggaagccac cagttgagac aggtccaggt ttaccgccac gcttccatcg ccgaggatcg
     4681 ataagccgac cactccgcgc acgtggcgag cgtacaggcc cgggctcttg accagcagct
     4741 cacgcgagtc gatcagccgg tcgacggcca acgcctgtgt cttgtgatcc agccgcagga
     4801 tgacggcgtc gtactggtcc aagggcttgt ccgccgccag tggcaggccc gcggcttcgg
     4861 ccaggtgcaa tgcgggcagc acacgcttgc catagcggta gaccagctgt tcgcccaggt
     4921 gctcgaagct gccggcgccg cgcggcaccg cctgctcgat gttcagcgac ggcagtgcga
     4981 accgctggcc gccggcttcc acgatcagcg actggatcgt cgacagcgac gccgcgaagc
     5041 gcagctccac gatgcatccc tgcccaatgc gtgactggat gcgcaccgaa ccgcccatgg
     5101 tcgcggccca gtcgcgcacc acgtccaggc ctaccccgcg gccggaaact tcgctgacgc
     5161 agtcgcgtgt cgagaagccg ggcagcagca cgaggcgcga gacctcgtcg tcggcgagcg
     5221 gcgcgtcggc gtcggccagt ccccgctcgg cggcacggcg gcggatggcg ggcaggttca
     5281 ggccgcgccc gtcgtcgctg cagcgcagca ccacctgctg gccctggcgc gagaacgcca
     5341 gctcgatatg gccggtctcg tccttgccgg ccgcgacgcg ttccgcgggg ggctccacgc
     5401 cgtggtcgac ggcattgcgc agcaggtgca gcagcgggtc ggccaggcgg ttgagaacgt
     5461 cgctgtcgat ctgcgtgtcg ccgcccttga gcaccagcac ggcctgcttg ccggtggcct
     5521 ggcaggtcga gcgcacattg cgctgcaggc gcgactcgag cacggcggcc gcggtcatgc
     5581 gcgttccgat caccaggtgc tgcaagtcct tggccagtcg atcctggcgc gtctgcacgc
     5641 tcccgagtcc ggcgataccg tcttcgagcc gcaaggccag gacgcgcgcg tcggccgctt
     5701 cctcgatcag cgcgtgcgcg gtgttgtgca gttcgctgta ctggtccatt tccagcgggt
     5761 cgaacgcttc ctcgtcggcg ccccgcgtgc gggcccgcat catcgtcagc gcgcgcacgt
     5821 cgaccagcgt ttccagctcg aacaggcgct tttgcacccg caggttttgc gccagcaatt
     5881 cgcgcgagcg ctccgccagc gccttgatgc gcgcctccat ggcggcactg tggaccgaca
     5941 cttcgcccga gacgcggaac agttcatcga cgcgctccat gctgacccgc agcgccgcgg
     6001 tcggcgcggc ggtcgccgcg gccgccggcg cgcgatcagg ttcggattcg cgagccgccg
     6061 catccgcaac cgcggccggc acgcgcgaga caggcgactc gagcgactcg ccgcggtcga
     6121 tccggttggc aaggtcgagc acgctttgca gcacggcctg cgcttgctgc gggtactcgt
     6181 cgctgcccgc gacatagccc accatctgct ccaggcagta ggcggcgtcg agcagcgcat
     6241 cggcggcggg cttggcgacg tgaccgccct cgcgctcgaa atgctcgagg atgtcctcga
     6301 agtggtggcc cagggacgcc aggccgcgca ggccgatgat ggcgccggaa cccttgagcg
     6361 tgtgcgccac ccgcttggcc gcgacgatgt cggaatgatc gccctggccg gcggccatgt
     6421 tgcgcgccag gtccaccagg tagcgggcgt ggtccggcgc ctcctggaaa aatccttcca
     6481 gcagcttctg gtcgacgtcc tggggcatga ccagcgccac gtcgtcgggg gtggccagca
     6541 ccggccgcat ttcctcgcca ccggcgccgc catggggttg catctgctgc ggcatcgcac
     6601 cgagcatgtg catcactttc agcgactgct cctcgtccag cggatagggc gctttgacca
     6661 ggtgatcgac caggccggcg gccgccgtgg gctcgcccat gcggcgcagg tagtacacga
     6721 tcaggggggg ccagccgcgc aggaactcga ccagcggccc gcgctccgcg ggcgccagca
     6781 tgggcatggc gaacgtgttt tccagcacgt gattgcagac cgcctgcagg cccgggaaac
     6841 cagccagctc ggcggcctcg cccatgcgct gggcctggcc gccgtactgc tccagggcgt
     6901 ccatgaatcc cgggtcttcc gcgtcgaggc cggccagctc ttcgaggctc tcgacgagcc
     6961 ggggctgggc catctccacc tctgcggcga gcgcctcgat caggtcgttg agtttcacgg
     7021 ggcttgtcct ggagcgtgcc gcgggtgggc ctcaggctgc ggccaacggc agcttgaaca
     7081 cgttcaccga ctccaccagg cggttggccg actgcagcag cgattcggtg tcgacgttct
     7141 gcgcgtcgat ctgttgcgag gtgcgctcgg tgctctcgcc catgcgctgc accgagccca
     7201 gcaggttggc cgacatcgat ttctgcaggt ccgacgcgtc gccgatgcgc ttgacctgcg
     7261 ccaccagctg gcccgtgatc tcctgcgtgc ggcgcatctg ctccccggcc ttttgcgcct
     7321 gctccgagcc ctgcaccacc tgcccgatgg tgcggttcac ggtaccgatg gtctcattgg
     7381 tttcgagctg gatgttgttc accagcgtgg cgatctgctg ggtggctgtg cgcgagcttt
     7441 ccgccaggcg ctgcacttcc tcggccacca ccgcgaaacc ccggcccgct tcgccggcca
     7501 cggccgcctg catcgacgcg ttcagcgcca gcacgtgcgt gcgttccgag atggtgttga
     7561 tcaggttcac gatgcccgag atttcctgcg agcgctcgcc cagccgcttg atccgctttt
     7621 cggtctcggc gatcatttcg cggatcgact ccatgcccct gacggtgccg ttcaccgtgt
     7681 ccagcgcggt gtcggtcgcc tgcgtggcct gctcggccga ggcattggac tgctcggcca
     7741 gtgccgagat ctggttcatg gtctgcgtgg cgtccgccag cgactcgatc atctgagcca
     7801 cgttcgtgcg ctcgtccgca gccgtcctgg acacgagcgc ggcctgcgcc tgcaccttgc
     7861 cggacgcctg ggccacctgg cccgcgatcc ggttcacgcc gtgcagcacc ttggtcgtct
     7921 cgtcggtcaa ggcattgatc gagtccccga cggttcccat cacgtcctcg gtcaccggcg
     7981 cgcgcacggt gaggtcgcgc tgcgagagca ggttcaccga ctgcagaatc gcgatcacgg
     8041 agttgttcaa gcgcatgttt tcgtcttccg cggcgcgctg cgcggcgatg cgctcgtcga
     8101 gcaggcggtc gaagttcttg gcgaattcac ccaactcgtc gtcggtcgtg agcccggcgc
     8161 gcgcctcgta gtcgccggcc gagaccttct cgatcgtgtc gtgaagcctg gcgagcggac
     8221 ggttcacgtc gcgatacagg aagaaggcgg aagcgccggt cgccagcacc atcgttatag
     8281 cggcgagcag caggaagatt tccccagtct tctttagctc ttctcgcttc accacgtcgc
     8341 tcttgacggt ggtttccaac aggtcgttca gccccttcac cgtctcggcg gccgccggat
     8401 tggtttcagt ggcgcgaaag accgaggctt cctcgagctt gcccttgtcg accaggtcga
     8461 accagacggg gcgcttttcc ttgtacttcg cgtagccttc caacatccgc ttgtgccctt
     8521 gcacttcctc gggcgtcaat ccctccatcg cggcgaatgc cttgaagttc tcttccacaa
     8581 tcgcaagctg cttcgggccg gcttcgttga tccgctggcg gttggccgca tcgggataca
     8641 ggacgtattg cgccaaatgg aagcgcagtt cccacatcgc attgttcgcg cgagcaagca
     8701 ttgcggtctt gccctgcacc tgtttctgca gggaggtcag ctcgcccatt tcccaagccg
     8761 ccaaacgggc ggacaggccc gccagcacca acagcccgcc gaacgcgatc agtagtttcg
     8821 ttcggatctt cagattcagg tccagtttca tcacgtcact ccagtcgttt tcgtttcttc
     8881 ggtgcgcccg gtggcgcctc gcgcgagccc gctattgagc ggtgcccgcc aattcttcgg
     8941 ccaaggttcg caacaggtgg tccacctgca ggtccatcca gtcgagcccc tcgctccaat
     9001 aggcatggtt cacgcagccc gccagggacg gcggcattgc ggcgtcttcg atccggtcgg
     9061 attccgtcag gcgcagccgc tgcggcaagc catcgatcac cacgcccgcc ttctcgtccc
     9121 cgtggccgag aaccaggagc attggtttcg ctttttcgtc gtgcgccacg ccgaaaagct
     9181 cggccgggtc gaacacgggc accagggtgc cgtgcaggtt ggccatgccg cggaaccacg
     9241 ccggcgcccg cggcagcagg tacacctgcg gcaggtccgt gagttcgttg ccgtcctggt
     9301 aacgaatcat caggcgcagc tcgccgatgc gaaaaccctc gcgcagcaag atggcgcccg
     9361 gcgcgtcgcc ggccgcgcgg accgggaccg cctccggcac ggccgccggc gtatcgaaca
     9421 cgaagccgcg tagcagcgcc tccgtcggtg cgagcagggg cgcgggcgcc gcggcgccgt
     9481 gcagacctcc ccctgcttgc atggcgccgg tcctcaggcg ttgagttcgg ccacgatctg
     9541 ctcgcggctg tacggcttgg tcacgtagcc cctggcgccc agcatctggc cccacgccat
     9601 ctcggcacgc tggttcttgc cggtcacgaa cacgaccggg atgtgtttgg tggcgtcatc
     9661 ggccttgagc ttgcgcgtcg cggcgaaacc gtccatgtcg ggcatgttca cgtccatcag
     9721 gatcaggtcg ggcttgtcgc gcttcgcgcg ctctatgccc tgggcgccgg agtcggcgag
     9781 cagcggcgtg tgacccgcgt ccgagacgat ccgctccagg ttggtgcgat cggcgagggc
     9841 atcatcgatg atcaggactc ggcggaacat ggaagtctcc ttcggggtaa agcggggaac
     9901 ggatttggtg cgcagccatg acggcattca cacggggcgc gtcgcggcga aacgcgccgt
     9961 gatgcgggga atcgaggact cggtgcgggg cgcggcgggt ggttcgcggc gctccggcat
    10021 cgcgctcagc tgttgcgcca gcacttcgcc gaggtgctcg gggtcgaccg gcttggtcag
    10081 gtaggcatcg cagccggcca tcttgccgcg gatgcggtcg aacggcgacc ccttgctggt
    10141 gagcatgacg accgggatgg cgttggctcc acgcagccgg gacttgatct ggcggcagcc
    10201 ttcatagccg tcgacctcgg gcatcagcac gtccatcagc acgcagtcga aggagcggcg
    10261 ggccacgctt tgcagcgcca cctccacgct gtcggcgtcg gtgacgacga agccccgggg
    10321 ctcgagcagc gagcgtagat gcgcccgcac ggccaggctg tcgtcgacga cgaggatctg
    10381 ggggtcgcgc accggtcgcc ttgccggcgc ggcggcaaga tgacccgcca acggcgcggc
    10441 ggcggcggtc ccggggcccg attcgagcgc ccgggcgagc accatgggaa gaatgggcca
    10501 ttgcaccggg cggcgcagca gcgtatggcc ggcaggcgct tcggtcccgt cgatccagat
    10561 caccgccctg cgctcgatcc acgggtggcg cttggtccag gccacggcgg ccgcatcgcg
    10621 ggtgtcgatg acgatcacgt cggcgtcggt ggcttgtttg cccggcagga tctccaggcg
    10681 cggcgtgcgg cgctgcgaga ccttcaccag gccctcgagc agttggcgct cgatcggctt
    10741 cagccctttg accaccagct tgagggacgg tgacggcctg gcggcggggc gagggggggc
    10801 tttcgcaggt gggggttctg tcgggccgaa aatggacggg ctcaagagct ggtcggcggg
    10861 cggctgtgcc gcgtcatgcg acccagcgcc tggcgggctg aaggaacccg cgtgggcatt
    10921 cgagttgttg ttgtccatac ttattagtag tcgtccactg ctccctgtgg ccggcccgac
    10981 gagtcctgcg cctctttcgg tctcggcccc ggtctcttgc tgatcggtgg actacccaac
    11041 cagtaaagtt agggtaacag cctattgcat tcgaaacatt gcgctgggtc aaaggggtct
    11101 ttgggaccac tttcggccgc tgttgacaag cttttggttg aacccaacgc ggctgctccc
    11161 tcgcgccaca acgacatggt gcagatcttc tacagctggg gcctgccggg attgcttgcg
    11221 tacctgagct tctgggtcgc attgctcaag cgggtctatt cacgcttttg ggtcaacggc
    11281 gaatactggc cggcgtgcgc gttggttgcg ctgtttccca gcatggtcac cgacgtgggc
    11341 ttccagcact acgagaaggc ggctttcctg gtccttctct gcgcgttttg cattgcctgg
    11401 ccggagaaga aagcggccgc cgcaggtccg gcgctcgcgc cgcgagcggc ggacacggcg
    11461 ccctgcgatg cgcccctcaa gccttgaacg gcaccaccag ctggcgctgc tcgcccaggc
    11521 cctcgatccc cagcgttatc acgtcgccct tgcgcaggta agtgggcggc ttcatgccca
    11581 ggccgacgcc cggcggggtg ccggtggtga tgacgtcgcc gggcagcagc gtcatgaagt
    11641 ggctgacgta gctcaccagc ttcgcgacat tgaaaatcat ggtgcgcgtg ttgccggtct
    11701 gcatccgttt gccgttgaca tccagccaca tgtccaggcg ctggacgttc acgatctcgt
    11761 cggtcgtgac cagccagggg ccgatcggcc cgaaggtatc gcagcccttg cccttgtccc
    11821 attgcggtcc gcgctcgagc tggtactcgc gctcgctgac atcattgacg acgcaatagc
    11881 cggcaacgca ggccagcgcg tccttggtgg gcacatagcg ggcacgcgtg ccgatcacga
    11941 cgccaagttc cacttcccag tcggtcttga ccgaggcgcg gggcagcatc accgcgtcgt
    12001 tcgggccctg gatgcagctg gtggccttca tgaacaccac cggctccttg gggatagcca
    12061 cgccggattc cgccgcgtga tcggcgtagt tcaagccgat ggcgatgaac ttgcccacct
    12121 gcgacaccgg gcagccgaag cgcggcgtgc cgcgcaccag cggcagcttt tgcgggttgg
    12181 ccttgcggat gcgcgccagc ccgacggtac ccagctgggc cggaccgata tcgggcacca
    12241 ccgggctcag gtcccgcagc cggccctcca ggtcgatcag gccgggtttt tccttgccgg
    12301 ggttgccata gcgaacgagc ttcatttatt ttttcctttc ttgccgatta gctgaaagct
    12361 gtcatcccga gtttgacccc tcacttgtca tcccgggctt gacccctgac ttgtcatccc
    12421 gggcttgacc cgggatccat gaattcagga gtcatggatg gcggggccgg gccggagcgc
    12481 gatgcctcgg tacgtgcata gcgtaggccc ggcattgggc tcgctgcact ggacagctgc
    12541 gcgccccggg cgttccgcga gcccagcccg caatgacaac gcacgggtca agcccgcaat
    12601 gacaatgcac gggtcaagtc cgcaatgaca atgcacgggc caagcccgca atgacggact
    12661 caataagtgg accttccacc cgaaagatcg aacacggcgc cggtggagaa cgagcactcc
    12721 tcggtgcaca gccatgcgac cagggccgcc acctcctcgg gcgtgccgaa gcggcccatg
    12781 ggaatcttcg agagcatgaa ctggatgtgc tgctcgctca tctgatcgaa gatcgcggtc
    12841 ttcaccgccg ccggcgtgac gcagttgacc cgcacgcccg tgtcggcgag ctccttgccg
    12901 agcgacttcg tgagcgcgat cacggcggcc ttgctggcgc tgtaggcact ggcgttcggg
    12961 ttgccgtcct tgcccgccac ggaggcgatg ttgacgacgc gtccatagcc ggcgtcacgc
    13021 atcagcggca cgatctcgcg gcagcaatga aacagtccat tgaggttgac ctcgatcacc
    13081 tgccgccagg cgcccaccgg atagtcccag accttgatat tgggcccggt gatgccggcg
    13141 ctgttgatca gcgcgtcgac acggcgcgtg tgccgcaagg tcgactggac ggcgcgctgc
    13201 accgattcgt gccgcgcgac atcgacctcc acggcctgcg cattggcgcc cagcaccccg
    13261 gcggcgcgct gcgcggcatc gaaatcgcgg tcccacaggg tgacgccgcc tccggaggcg
    13321 atcagccgcg ccgcaatggc gtagcccagg cccgcagcgc cgccggtgac cactgcgtgc
    13381 cggccctcga aatcgaggcg gttcatatgg tgatgccccc gtcgaccgca tacgcctggc
    13441 cggtgacgaa ggtcgattcg tcgctggcca ggaacacgac caacggcgca atctcgtggg
    13501 cctgtgccag ccgccccatc ggctggcgcg cgatgaaatt ctttcgcgcc gcctcggggt
    13561 cctcgtaggc gttgatgcgg tcctgcagcg aaggcgtgtc gacggtgccg ggacagatcg
    13621 cgttgcagcg gatgccgtcc gccacgaagt cggccgccac gctcttggtg aggccgatca
    13681 ccgccgcctt ggtggtgccg tagatgaagc gattgggcag gcccttgatg ctgctgcaca
    13741 cgctggccat gttgatgatg ctgccgcgcc cggcggcgac catcttgggc agggccgcct
    13801 ggatggccca gaactgggag cgcacgttca ggttcaacgc gaaattccat tcctcgtcgg
    13861 tcgcctgcag ggccgttccg ctgtgcacga agccggcgca gttgaacagc acgtccaggg
    13921 cgggcaggcc ggaaatcagg cgttcgatgg cgcctttgtc gagcacgtcg agtgcagcgg
    13981 tgcgcacgcc cgcgatgccc tcataactgg cgagcaggtt cgcgtcgaca tcggtggccc
    14041 agacctgggc gccctccgcg gccagcgcca gcgcgctggc acggcctatg ccttgacccg
    14101 ccgcggtcac cagggcggtc tttccttcga gtctcatcgc ttctccacag ggtgtgccgt
    14161 ctcggcccgt cgttcagggt tttgccctat ggcgcaggcc gcgtttgcct cgatcataat
    14221 tggtctgacc acttggccaa ttagcaccaa ccccaaccca gcccttattt gatttgtcat
    14281 cccgggcttg acccgtgcat tgtcatcccg ggcttgaccc ggaatccatg aattcaggag
    14341 tcatggattg cgggtcaagc ccgcaatgac aagatttttc agcacctagc cccctagctc
    14401 atgccgctgc aaaccgtcga accgcaacgc ctgtaccggc agatcgcgga gcagttgcgc
    14461 tcgctcgtcg ccaccggcga gttcagcccc ggcagccggc tgccggccga gcgcgacctg
    14521 gcgcggcaac tgggcgtcag ccgcccttcg gtgcgcgaag ccctcatcgc cctcgaggtc
    14581 gagggcgtca tcgacgtgcg caccggctcc ggcatctacg tgagagcgac ctacaccgcg
    14641 aacggagccg gcacggcacg accccaggag cacgatcccg gcgaatgggg accgctggaa
    14701 ctgatgcggg cgcgcgaact ggtggaaggc gaagtggctg ccctggccgc gcgcaacgcg
    14761 cgccgggcac agatcgcggc catcgccggc gccatggcgc agatgcgcga ggaagccgcc
    14821 gccggtatca ttccgcgcgc cggcgacgag gccttccaca ccgctgtcgc acaggcttgc
    14881 ggcaacgaag tgctgcacga caccatccgc agctactggc gggcgcgcag cggccccttg
    14941 ttcgagcgcc tgggcgacta tttcgagaat cccgcctcct ggaacgccgc cctggtggag
    15001 cacaccgccg tgctcgaggc cattcgcggc catgacagcg cggccgcgcg gctggccatg
    15061 caagcgcacc tgaagaaagc ctgttcccga tacagcgcga gctggcgccg cgcgaatagt
    15121 tcctgatccc gcccagcatc aacacaaagg aaaccaccat gaccaaacgc cacaccctca
    15181 aactcatcgc cgcgtgcgcg ctgctggcca cagcggctgc gcctttcggc ctggcccagg
    15241 cgcagaccaa gctgaaatgg gcccacgtct atgaaacctc cgagccgtac cacaaatggt
    15301 cggtgtgggc cggcgaggaa ttcaagaagc gcaccaacgg caagtacgag atcacggtgt
    15361 tcccggcctc cagcctgggc aaggaaagcg acatcaacca gggcctgcag ctgggcaccg
    15421 tggacatcat cctcaccggc gcgtctttcg ccgcgcgcac cttcccgcgc ctggccgtga
    15481 gctactaccc gtttaccttc cgcgatgccg accacgtgct caagtacgcc aagagcgacg
    15541 tgttcaagga actggccgaa ggctacaaga aggccagcgg cggcaacacc atcacggcaa
    15601 tgacttacta cggcgtgcgc cattccacca gcaacaaggc gttcaagaac tgcgacgaga
    15661 tgaagggcct gaagatgcgc gtgcccgacg cgccggccta caccgcgatg ccgcgcgcgt
    15721 gcggcgccaa ccccacgccg atcgccttcg ccgaggtgta cctggcgctg cagaacggca
    15781 ccgtggacgc gcaggagaat cctctgccca ccatcgaggc caagaagttc tacgaagtgc
    15841 agaagaacat catcctcacg ggccacatcg ccgacgcact gctcaccatc gtgtcgcccg
    15901 gcctgatgtc caagctgacg ccggccgaac agaagatcct ggtggacctc acccaggagg
    15961 ccgcggagaa agccagcaac gacatccgca agcgcgaggt cgagctggtg gacgagttca
    16021 agaagaaggg catcaacgtc atcaccgtcg atcgcaacga cttccagaag cgcgtgctca
    16081 aggccatccc gttcgagtcc atgggcctgg acaagaagga ctgggaccgt gtcatcgcca
    16141 tcaagtaagt ctcaggccgc gacgatcgac gatctgcccc cggtgatggg ggcggacggt
    16201 gagttccacg tgtccgacga ggccgtggac ctctccggca ccaccgtgga agcctggctc
    16261 gcgctgggct tcttctgggt gctcggagcc acggtggtct accagttcgt cacccgttac
    16321 gtgatgaacg actcggcggc ctggaccgag gagatcgccc gctacctctt gatcgcgacg
    16381 gtgttcatcg gggccaccat cggcgtggtg aagaacaacc acatccaggt ggattttctc
    16441 taccgctata tgccccgcgg catggcccgc ttcatggcca cggccgtgga catcatgcgc
    16501 atcgcgttct tcgcggcggc ggcggtgttg acggtgatga tgatgctcaa gctgggcagc
    16561 aactcgcgca tgacgctggt cgacttgccg atgaactggg tctacggcat gtgcctgctg
    16621 ggtttcgcgg cgatgacgct gcgctcgatc caggtggcat ggatccatcg aaagcgcggc
    16681 tacagcgtgc tggaacgccc cgaatcgcac atgacggacc gatagctcgc gggccgcgcc
    16741 gcacgaacca agacactcga cccctcatgc tcaagttcat tttcctgctg ttcatgggca
    16801 gcggccttcc ggtggcgatc gccatggcgg gctcggtgct ggtctacatc ctgtggaccg
    16861 gcaacctgcc ggcttttgtc gtcatccacc gcatggtggg cggcatcgac agtttcccgc
    16921 tgctggccgt gcccttcttc atcctggccg gcaacctgat gaacaacgcc ggcatcacca
    16981 atcgcatcta caactacgcc ctggcgctgg taggctggct caagggcggc ctgggccacg
    17041 tcaacgtgat cggctcggtc gtgttcgccg gcatgagcgg gacggctatc gccgatgcgg
    17101 ccgggatcgg caccatcgaa atcaaggcca tgaaggacca cggctacagc tcggagttcg
    17161 cggtgggggt gacagccgcc tccgcgacgc tggggccgat cattccgccc agcctgccct
    17221 tcgtgatcta cggaatgatg gccaacgtga gcgtgggcgc cttgttcctc gccggcatcc
    17281 tgcccggcgt gctgatggcg ttgctgatga tgctcaccgt cgcgtactac gcgcacaaga
    17341 acggctgggg ttcagacatc aagttcgagt ggccgcgctt caccaaggca attgccgaga
    17401 cgctcgtggt gatcggctgg ccggtggccc tctggctgct gattgtgaaa gtggggctgc
    17461 cgccccaggc cacggtcggg gccgggttgg tgatcctctt cgccgccgac aagatcttca
    17521 gcttccaggc cgtgctgccg atcatgaccc cggtgctgct gatcggcggc atgaccaccg
    17581 gcatcttcac tcccaccgaa ggcgcaatcg cagcctgcct gtggtcgatg gtgctgggct
    17641 tcggctggta cggcacgctc agctggaaaa agttcgtcaa gatctgcctg gacacggtgg
    17701 aaaccaccgc caccgtgctg ttcatcgtcg cggccgccag catcttcggc tggatgctca
    17761 ccgcgaccgg cgtcacggcc gacatcgcgg cctgggtgct gggcttcacc aaggaaccct
    17821 gggtatttct gttgctcgca aacctgctga tgctgttcgt cggctgtttc ctggagccga
    17881 ccgccgccat cacgatcctg gtgcccatcc tggtcccgat ctgccagcag ctgggcatcg
    17941 acctggtgca cttcggcctg gtcatggtgc tcaacctgat gatcggcctg ctgcatccgc
    18001 ccatgggcat ggtgctgttc gtgctggccc gggtcgcgaa gctctcggtg gagcgaacca
    18061 ccatggcgat cctgccctgg ctggtgccgt tgctcggatc gctggcggtc ctcacgtact
    18121 tcccgaagct cgtgatgtgg ctgccgaaga tgttttactg aaacctcggg ggacaggact
    18181 tcagtgctgt tccgccgaac gctcgagacg ttgccaatga cacctttcat ccgcctccac
    18241 ccgcaggacg acgtcctgat cgcaagaacc cagctcgtcg gcggcgccac ggccgaaaac
    18301 gtcgtcatca aaggcctgat cccggcgggc cacaaagtcg ccacgcgcgc cattgcgcag
    18361 ggcgagccgg tgcgccgcta caaccagatc atcggcttcg ccagcaagcc gatcgctccc
    18421 ggcgagcatg tgcacacgca caacctcgcc atgggcgatt tcgagcgcga ctacgccttc
    18481 ggcgcggatg tgaaacccgc ggcgccgcgg cgcgaggcga cgttcatggg catcaagcgc
    18541 gccgacggcc gcgtggccac ccgcaacttc atcggcatcc tgtccagcgt gaactgttcg
    18601 gcgaccgcgg cgcgggcaat cgccgattac ttctcgcggc agaaccacgc cgacgcgctg
    18661 gccgcatttc ccaacgtcga tggcgtggtc gccctcaccc acggcaccgg ctgcggcatg
    18721 gccaccgagg gcctgggcat gcagatcctg gagcgcacgc tggccggcta cgccacccac
    18781 gccaacttct ggggcgtgct ggtggtgggc ctgggctgcg aggccaacca gatcaacgcc
    18841 tggctggcgc acagttcgct gcgcgagggc gagacgctca aggtcttcaa tatccaggat
    18901 accggcggca cgcgcaagac cgtggagaag ggcatcgccc tgatccgcga aatgctgccc
    18961 caggcgaacg cggtgaagcg cgagccctgc agcgcgtccc acatcacgat cggcctgcaa
    19021 tgcggcggct cggacggcta ctcgggcatc agcgccaatc cggcgctcgg c
//
LOCUS       DASWIY010000148         6690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_455317, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000148 DASWIY010000000
VERSION     DASWIY010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455317"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="VGA59_05630"
     CDS             <1..1502
                     /locus_tag="VGA59_05630"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX9719192.1"
                     /translation="VTAITPTPAGEGKTTTTVGLGDALNKIGKKAILCLREPSLGPVF
                     GMKGGAAGGGYAQVVPMEDINLHFTGDFSAIGLANNLLAAAIDNHIHHGNELGLDVRR
                     ITWKRVVDMNDRALRDITVSLGGPGNGYPRQDGFDIVVASEVMAIFCLATSLKDLKNR
                     LGNIVVGYTRDQKPVRARDLKVHGAMTVLLKDALAPNLVQTLENNPAFIHGGPFANIA
                     HGCNSVIATRTALKLADYCVTEAGFGADLGAEKFIDIKCRKSGLRPDAVVIVATIRAL
                     KHHGGVDRADYNKENLAALEKGIANLERHVNNVRNHYGLPCVVSINRFNFDTPAEIEL
                     LKRKMAHHEAPVLLATHWADGGKGAEQVARTLVDLIEKVPSDFKYVYDDAMPLWDKIR
                     TVATRIYGASDVTADGKIRAQIKKLQEEGYGSYPVCIAKTQYSFSTDPQLRGAPSGHV
                     INVREVRLAAGAEFIIMVCGDVMTMPGLPKVPSAEKIDLTDDGKVVGLF"
     gene            1579..2319
                     /locus_tag="VGA59_05635"
     CDS             1579..2319
                     /locus_tag="VGA59_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX9719193.1"
                     /translation="MEFLNELQTTAFWVGLWQIILVNIVLSGDNAVVIALAARSLPPK
                     QQTQAVFWGAGAAVVMRIILTIVAVELLKWPWLKLIGAVLLLWIAVKLLLPEDGNEGD
                     VESSENLWAAIKTILIADLVMSLDNVIAVAAAAKGSLVLLILGLAISIPLVIFGATML
                     MKIMDRYPVIITIGAGLLGWVAGDMVATDPVVKDWVDANAAWLHWAAPAAGAVFVVAA
                     GRWLAARAEAGRKEVVDLGEGSETKVPH"
     gene            2383..2814
                     /locus_tag="VGA59_05640"
     CDS             2383..2814
                     /locus_tag="VGA59_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9719194.1"
                     /translation="MLKFLVAVDGSAPSSRAVDHLIRQVGVLKEEAEIHLLNVQHQIP
                     YGSRVSSVVGHDKIAQFHREEGMAALKTAMQKLDSARIKYHHHIGVGAEADVICQYAR
                     EKGCDQIFMGTRGLGSVSNLVLGSVATKVIHLSPVPVVLVK"
     gene            3002..4000
                     /locus_tag="VGA59_05645"
     CDS             3002..4000
                     /locus_tag="VGA59_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9719195.1"
                     /translation="MRIQRNLTVSAVLLAATLTGPAAAWEPSKTVEFIIPAGTGGGAD
                     QMARFTQGVVAKHSLMAQSIVPINKSGGAGAEGFLDVKASKGDEHKIIITLSNLFTTP
                     LATGVPFNWKDLTPVAMLALDNFVLWVNAETPYKSPKEYLDAAKKAGPGKFTMAGTGA
                     KQEDQIITVAIEKAAGVKFTYVPFKGGGAVATQLVGNHVNSTVNNPIEAVAHWRSGKL
                     RPLCVFEKARMPYKNKVTSTMSWNDIPTCKEMGIPMDYLMLRGIFMPAGVSQDVVNFY
                     IGFFKKVRGTAEWNKLMEDGAFNQSFMTGKEYVNWVAQAENTHRELMREAGFLAKK"
     gene            4106..4612
                     /locus_tag="VGA59_05650"
     CDS             4106..4612
                     /locus_tag="VGA59_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX9719196.1"
                     /translation="MENKNESAHRAVRIVTMDVVVAALIFALGALMIYESNRLGSGWD
                     ENGPQAGYFPYYTGLLVCISGAVVFVQGLFKLKSDGRVFVRLDQFKQVLVILLPSAAY
                     VLGVQLIGIYVPSAVFIGLFMKIVGKYRWLRSALVGLSVSVIAFLLFEVWFKIPLPKG
                     PLERLLGY"
     gene            4725..6233
                     /locus_tag="VGA59_05655"
     CDS             4725..6233
                     /locus_tag="VGA59_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX9719197.1"
                     /translation="MEELNALFHGFTVALTPFNLMLMFVGIILGVIIGVLPGLGGANG
                     VAILLPLTFSMPPTSAIIMLSCIYWGALFGGAITSILFNIPGEPWSVATTFDGYPMAQ
                     QGRAGEALTGAFTSSFVGALVAVLMFTFLAPLIANFALQFGPPEFFAVYLLTFCSFVG
                     TGKGNPFKIVASMMLGFAMAAVGIDIVTGQLRLTFGFTELLSGFHFLVAVIGLFGIGE
                     ILLSMEEGLSFSGHGARINPTIVLQTWAKLPRYWATSIRSCFIGCWMGIIPGGATPAS
                     FMSYGVARQMSREGAKFGTGQLEGVVAPETAAHAAGTSALLPMITLGIPGSPTAAVLL
                     GGLMIWGLQPGPLLFVEQKDFVWGLIASMYLGNIAGLIVVLTMVPLFAAILRVPFPII
                     APVIVVICAIGAYTVKNNMLDVWMTVVFGMIGYVFKKLDYPLAPMVLALVLGDAAESS
                     FRQAMLIAQGDLTVFWKNWLVGSLTTLALVLLFWPFISAAMTRLRALAPATR"
     gene            complement(6247..>6690)
                     /locus_tag="VGA59_05660"
     CDS             complement(6247..>6690)
                     /locus_tag="VGA59_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9719198.1"
                     /translation="SMEQYFIIDGILKRVVSNARGKEMILRFAAESDIDTSYAAWRLK
                     TPIPYSIRAVTRVRVAKLSMPRWVQFLERHPSVKSVFEFEIMKLMSEVMAHTITLHLL
                     DAPGRVQRFSRKHAALAGRLPKKELASYLNLSPETLSRLKRHGKI"
BASE COUNT         1235 a         1980 c         2215 g         1260 t
ORIGIN      
        1 tggtcaccgc catcacgcca acgcctgcgg gggagggcaa gaccaccacc accgtcgggt
       61 tgggagacgc gctcaacaag attggcaaaa aggccattct ctgcctgaga gaaccgagcc
      121 tcgggccggt attcggcatg aagggcggcg ctgccggcgg cggttatgcc caggtcgtgc
      181 cgatggagga catcaacctg catttcacgg gcgacttcag tgccatcgga cttgccaaca
      241 acctgctcgc agcggcgata gacaaccata tccatcacgg caacgagctg gggctggacg
      301 tgcggcgcat tacctggaag cgcgtcgtgg acatgaacga ccgcgcgctg cgcgacatca
      361 cggtctcgct cggaggtccg ggcaacggct acccgcgcca ggacggtttc gacatcgtgg
      421 tggcctccga ggtgatggcg atcttctgcc tcgccacttc gttgaaggac ctcaagaacc
      481 ggctcggcaa catcgtcgtc ggctacacgc gcgaccagaa gccggtgcgg gcgcgcgacc
      541 tcaaggtgca cggcgcgatg acggtgctgt tgaaggacgc cctcgcgccg aacctggtgc
      601 agacgctgga aaacaacccg gcgttcattc acggcgggcc gttcgccaac atcgcgcatg
      661 gctgcaactc ggtcatcgcc acccggaccg cgttgaagct cgccgactac tgcgtgaccg
      721 aggccggctt cggcgccgac ctcggggcgg agaagttcat cgacatcaag tgccgcaagt
      781 ccggattgcg ccccgacgcg gtggtgatcg ttgccaccat ccgcgcgctc aagcatcacg
      841 gcggcgtgga ccgcgccgac tacaacaagg aaaacctggc ggcgctggag aagggcattg
      901 ccaacctcga gcgccacgtc aacaacgtgc gcaatcacta cgggctgccg tgcgtggtct
      961 cgatcaatcg tttcaacttc gacacgccgg cggagatcga gctgctcaag cgcaagatgg
     1021 cgcaccacga agcgccggtg ctgcttgcca cgcactgggc ggacggcggc aagggggcgg
     1081 aacaggtggc gcgcacgctg gtggatctca tcgagaaggt gccgtcggac ttcaagtatg
     1141 tttatgacga cgcgatgccg ctgtgggaca agatcaggac cgtcgccacc aggatttacg
     1201 gagcctcgga cgtgacggcg gacggcaaga tccgggccca gatcaagaaa ttgcaggagg
     1261 aaggatacgg gagctatccg gtgtgcatcg cgaaaaccca gtactcattt tcgaccgacc
     1321 cgcagctgcg gggtgcgcct tcgggccacg tgatcaacgt ccgcgaagtg cggctcgcgg
     1381 ccggggcgga attcatcatc atggtgtgcg gtgacgtcat gaccatgccg gggctgccca
     1441 aggtgccgtc ggcggaaaaa atcgatctca ccgatgacgg caaagtcgtc gggctattct
     1501 gatctggtat gatccggcgc gccgacgcgg gcccaacagg atcatgaata acgatggagc
     1561 gaggagaaac cgcgaatgat ggaattcctg aacgagctgc agaccaccgc gttctgggtc
     1621 gggctgtggc agatcattct cgtcaacatc gtgctctccg gcgacaacgc ggtggtgatc
     1681 gctttggcgg cccgttcgtt gccaccgaaa cagcagacgc aagcggtgtt ctggggcgcc
     1741 ggcgcggcgg tggtgatgcg gatcatactc accatcgttg cggtggaatt gctgaagtgg
     1801 ccatggctca agttgatcgg cgcggtgctg ctgctgtgga tcgcggtgaa gctgctgctg
     1861 ccggaggacg gcaacgaggg tgacgtcgaa tcgagcgaga acctgtgggc ggcgatcaag
     1921 accatactga ttgccgacct ggtgatgagc ctcgacaacg tgattgccgt ggcggcggcg
     1981 gcgaagggca gtctcgtgct gctaattctc ggacttgcga tcagcatccc gctggtgatc
     2041 ttcggcgcca ccatgctgat gaaaatcatg gaccgctacc ccgtcatcat caccatcggc
     2101 gcggggctgc tcggctgggt ggcgggtgac atggtggcga ccgaccccgt ggtgaaagac
     2161 tgggtggacg cgaatgccgc ctggctgcat tgggcggcgc cggcggcagg ggcggtattc
     2221 gtcgtggccg ccggcaggtg gcttgccgcg cgcgccgagg ccggtcgcaa agaggtcgtg
     2281 gacctcggcg agggcagcga gaccaaagtg ccgcactgag acgagaacac tccctggtgc
     2341 aggctgacca atgcgattca atgagtaatc gggggcaaac ggatgcttaa atttctggtg
     2401 gcggtggacg ggtcggcgcc gtcaagccgt gccgtagacc atttgatcag gcaggtgggt
     2461 gtactcaagg aagaggccga gatccacctt ctaaacgtcc agcaccagat tccctacggc
     2521 agtcgggtca gttccgtcgt cggtcacgat aagatcgccc agttccaccg cgaagaaggc
     2581 atggcggcgt tgaagaccgc gatgcagaag cttgactccg cgaggatcaa atatcaccat
     2641 cacatcggcg taggggcgga ggccgacgtc atctgccaat atgcaagaga aaagggctgc
     2701 gaccagatct tcatgggcac ccgcggcctg ggctcggtgt cgaacctggt gctgggatcg
     2761 gtggcgacca aggtaattca tctgtcgccg gtgccggtgg tgctggtgaa gtgacggcag
     2821 aaccggcatt gttgcggcgc acaaatccgg gatcaaggat actgcccatt gaaaaattga
     2881 cgccggtcaa tggcattcga gacggatttc tggattatta tccagcccta gtggcagttg
     2941 ctgggatgga gtaggtgctg tgccagaacg gtttgtccaa cacaacgagg aggaatcagt
     3001 catgcgtatc cagcgaaatt tgacagtgag tgcggtgctg ctcgcggcaa ccctgaccgg
     3061 cccggcggcg gcgtgggagc cgagcaagac ggtcgagttc atcatcccgg cgggcaccgg
     3121 cggcggcgcc gaccagatgg cgcgttttac ccagggcgtg gttgccaagc acagcctcat
     3181 ggcacagtcg atcgtgccga tcaacaagtc aggcggcgca ggcgcggagg gcttcctcga
     3241 cgtcaaggcc tcgaaaggcg acgagcacaa gatcatcatc acgctctcca acctgttcac
     3301 gaccccgctt gcgaccggcg tcccgttcaa ttggaaggat ctgactccgg tcgcgatgct
     3361 ggccctggac aactttgtcc tgtgggtcaa cgccgaaacg ccctacaagt cgccgaagga
     3421 gtacctcgat gcggccaaga aggcgggtcc aggcaagttc acgatggccg gcaccggcgc
     3481 caagcaggag gatcagatca tcacggtcgc gatcgagaag gccgcgggcg tgaagttcac
     3541 ctacgtgccg ttcaagggcg gcggcgcggt cgccacgcag ctggtcggga accacgtcaa
     3601 ttccacggtg aacaacccga tcgaggcggt ggcgcactgg cgctccggca agctgcgccc
     3661 gctgtgcgtg ttcgagaagg cgcgcatgcc gtacaagaac aaggtgacca gcaccatgtc
     3721 gtggaatgat atcccgacct gcaaggaaat gggcattccc atggactacc tgatgctgcg
     3781 cggcatcttc atgcccgccg gcgtgtcgca ggacgtggtc aatttctaca tcgggttctt
     3841 caagaaggtg cgcggcaccg cggaatggaa caagctcatg gaagacggcg ctttcaacca
     3901 gagcttcatg accggcaagg agtacgtcaa ctgggtcgcg caagcggaga acacgcaccg
     3961 cgagctcatg agggaagcag gcttcctcgc caagaaatag cgccacctgt ggggacgggc
     4021 gtcatgcccg cccccatttt cttctccgca gcggagcgtc tgttggcgcc ccgtttctct
     4081 cgcgtttaca ggggcagcgg ctgagatgga aaacaagaac gaatcggcgc atcgtgcggt
     4141 gcgcatcgtc accatggacg tcgtcgtcgc ggcgctgatc ttcgcgctgg gcgcgctgat
     4201 gatctacgaa agcaaccggc tcggttccgg ctgggacgaa aacggtccac aggcggggta
     4261 tttcccgtat tacaccggat tgctggtgtg catctcgggg gcggtggtgt tcgtgcaagg
     4321 cctgttcaag ctcaagtccg acgggagggt tttcgtccgg ctggatcagt tcaagcaggt
     4381 gctggtgatc ctgctgccct cagcggccta tgtgctgggg gtccagctga tcggcatcta
     4441 tgttccgtcg gccgtgttca tcgggctgtt catgaagatc gtcggcaagt accgctggct
     4501 ccgcagcgcg ctcgtcggtc tctccgtcag cgtgatcgcg ttcctgctgt tcgaagtgtg
     4561 gttcaagatt cccctgccca aggggccgct ggaacgcctg ctggggtatt aatgagtacc
     4621 tcgattttgc aaggcgacac gaatccctca cccccgcccc tctcccgagt ggagagggga
     4681 gattcgaggt acaaacgcgt gaatgggcgt ttgtaaggga gacgatggaa gaactcaacg
     4741 cgctgtttca cggcttcacg gtcgcgctga cgccgttcaa cctgatgctg atgtttgtcg
     4801 gcatcatcct cggcgtcatc atcggcgtgt tgccgggact gggcggcgcg aacggggtgg
     4861 cgatcctgct gccgctcacg ttcagcatgc cgccgacctc ggccatcatc atgctctcgt
     4921 gcatttactg gggggcattg ttcgggggcg cgatcacctc gatactgttc aatattccgg
     4981 gcgagccgtg gtcggtggcg accacgttcg acggctatcc catggcgcag cagggccgcg
     5041 cgggcgaggc gctgaccggc gcgttcacct cctcgttcgt gggcgcgctg gtcgcggtcc
     5101 tcatgttcac cttcctcgcg ccgctaatcg ccaatttcgc gctgcagttc gggccgcccg
     5161 agttcttcgc ggtatatctg ctgactttct gcagcttcgt cggcaccggc aagggcaatc
     5221 cgttcaagat cgtcgcctcg atgatgctgg gctttgccat ggcggcggtc ggcatcgaca
     5281 tcgtcaccgg ccagctgcgg ctcacgttcg gcttcaccga gctgctctcg gggttccact
     5341 tcctggtggc ggtgatcggg ctgttcggca tcggcgagat actgctttcc atggaggagg
     5401 ggctgtcgtt ctccggtcat ggcgccagga tcaaccccac gatcgtgctt cagacctggg
     5461 ccaagctgcc gcgatactgg gcgacatcga tccgcagctg cttcataggc tgctggatgg
     5521 ggatcattcc gggtggcgcg acaccggcat cgttcatgag ctacggcgtc gcgcgccaga
     5581 tgtcgcgcga gggcgccaag ttcggcaccg gtcagctcga aggcgtcgtc gcgccggaga
     5641 ctgcggcgca cgccgccggc acctccgccc tgctgccgat gatcacgctt ggcataccgg
     5701 gctcgccgac cgccgccgtg ttgctcggtg ggctgatgat ctgggggttg cagcccggcc
     5761 cgctgctgtt cgtcgagcag aaggacttcg tgtgggggct gatcgccagc atgtacctcg
     5821 gcaacatcgc ggggttgatc gtggtgctca ccatggtgcc gctgttcgcc gccatcctgc
     5881 gcgtcccgtt tccgatcatc gcgccggtca tcgtggtgat ctgcgccatc ggcgcctaca
     5941 ccgtcaagaa caacatgctc gacgtgtgga tgacggtggt gttcggcatg atcggttacg
     6001 tgttcaagaa gctcgattac ccgctggcgc cgatggtact ggcgctggta ctgggtgacg
     6061 cggccgaaag ctccttccgc caggcgatgt tgatcgcaca gggcgatctc accgtgttct
     6121 ggaagaactg gctggtgggc agtctcacca cgctcgcgct ggtactgttg ttctggccgt
     6181 tcatttcggc cgcgatgacg cggctgcgtg cgctggcacc cgcgacgcgt tagaggagat
     6241 cagccgctag atcttgccgt gacgcttgag cctggacagc gtctccggcg agaggttgag
     6301 ataggaggcg agctccttct tcggcagacg gccggcgagt gcggcatgct tgcgcgagaa
     6361 gcgctggacg cgccccggcg catcgaggag atgcagcgtg atggtgtgcg ccatgacttc
     6421 gctcatgagt ttcatgatct cgaactcgaa tacgctcttc accgacggat gccgctcgag
     6481 gaactgcacc cagcgcggca tcgagagctt ggcgacgcgc acccgggtca ccgcgcggat
     6541 cgagtacgga atcggcgtct tcaggcgcca cgcggcgtaa ctggtgtcga tgtcgctctc
     6601 cgcggcgaag cgcaggatca tctccttgcc tcgggcgttc gacaccacgc gcttgaggat
     6661 gccgtcgatg atgaagtatt gctccatgga
//
LOCUS       DASWIY010000149         9282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1187716, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000149 DASWIY010000000
VERSION     DASWIY010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9282
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187716"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(461..1237)
                     /locus_tag="VGA59_05665"
     CDS             complement(461..1237)
                     /locus_tag="VGA59_05665"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX9719199.1"
                     /translation="MGFLAVDVGNTRLKWALYEAPSPGATLRAHGAEFLENIDKLADG
                     AWSALRPPERMLGCAVAADAVKRRVEEQMEELWDVPAHWVVPNAAEGGLVNGYDHPAR
                     LGADRWVAMIGARHRMLARGAPRPLVVVMVGTAVTVEAVDAQGKFLGGFILPGHGIML
                     RALESGTAGLHVPTGDVREFPTNTSDALTSGGTYAIAGAIERMVQHVRRHCGAEPACF
                     MTGGAGWKMAPHMSVAFELVESLIFGGLLEIARRRFEIPS"
     gene            complement(1296..2216)
                     /locus_tag="VGA59_05670"
     CDS             complement(1296..2216)
                     /locus_tag="VGA59_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719200.1"
                     /translation="MADHDRTASLMTPSKLAQIQPRTAVSPAAWLSRMAADAGHPHVR
                     RLRELHGELQAQALLRVDWPAVSELGRLAHALPGLDFGLLQDRGWWARTTGKSRSAGA
                     EFAAQFNRTDLVARALAGQTQALQKKQKEQATAVDFALLEVEVEYHAIDKIIDQGARW
                     LQDMRNQLKSRQAAPADAAAQEQIKEDEARCEILVARLKTLRAVSSAAQRTHQQGQGA
                     AARRSALLKLLQQALASGVKAWQESVSALAAAAADGSSPKLNMDGPMEAHRELQLSVK
                     QAVADCGQLQVQEEALAESVTALGTQLEAA"
     gene            complement(2275..2943)
                     /locus_tag="VGA59_05675"
     CDS             complement(2275..2943)
                     /locus_tag="VGA59_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9719201.1"
                     /translation="MGVPVNFDSVSLDGLLSDWVGALREFSVEAVAVLGPDPFGTSED
                     RQVVAVHPPLVADAAKALAESRDFGAPWRDSDAPLVAWQYIAKSDHLSSSRWRLLWLA
                     HGFQTVVRVEFSLPAGRAFECFMFSPRELTDRSEAAALVWSALNIWPLVKRCVANARS
                     TLSPRERECLALAFQGLTARESSVRLHCSERTVNYHLANAMGKLKVDNKMAAIQRACW
                     LGAI"
     gene            3039..4340
                     /locus_tag="VGA59_05680"
     CDS             3039..4340
                     /locus_tag="VGA59_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9719202.1"
                     /translation="MSSIRVLYSGAAELLKSWASNEVMRPLLEPILHPSRWRIRALGL
                     STVFGHPLFYWIWAKLLPQPYENLGLRLLMSGLGVSLLVFPSITASPPSKFAAAVFTV
                     IFWITLPWFFSWMYFCNNGNPVWLASLAAMFLIYYHLTDWRLATLGTTTGMMAAWILF
                     RAVGPQTPGLSVEEAATNGVVIAFSWGMGVVLGISSSNLRREQLDYTLGTMGIMAHEL
                     RTPLATMSLIGDAVRGAASRCESDPRQTLEQLASRLHALVRNMNHQIDTQIANARLMR
                     LPVHKEAVSAAELVREAVNTYPYRSSRERECVKLQVQNDFLFEGSHALFLQVVDNLLK
                     NALRSLAAAIAASQPGDITIEVGTAGNRGRIVVTDRGVGMDADLQARIFEPFFSTDRG
                     SGHGLGLAFCQRVIDGANGSIRVKSEPGHGASFTVELPILA"
     gene            4386..5363
                     /locus_tag="VGA59_05685"
     CDS             4386..5363
                     /locus_tag="VGA59_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719203.1"
                     /translation="MSFPLIHRPGTVVFLDDDPDYLEMLALVLPRHWHVKLFVRPVEC
                     INYLQQEPPFWEADAWNQQQLIDHWREGKRLIPQVLGYWSKYTERYALTRVCVFDYSM
                     PGMDGLQALGELVDWPGSRVLLTGQADEQVAVRAFNRGLIDQFIAKQTPDISRRLIEA
                     VEHLLATPNARHAQTWRVTLGPEQNALLRMPSVGRDLAGFAAKRWVEHVVIGDPFGVL
                     GMDAAGNVSWLQLETPEGLKAQAELAELGGVPPSGLDDIRQGLKLADLELRQGLGRTG
                     PPELAAAFPVGQDGAVLGALFAVDSPLCPDPGNSYSNWLARQSKRHVQD"
     gene            complement(5372..6610)
                     /gene="cqsA"
                     /locus_tag="VGA59_05690"
     CDS             complement(5372..6610)
                     /gene="cqsA"
                     /locus_tag="VGA59_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxyketone-type quorum-sensing
                     autoinducer synthase"
                     /protein_id="HEX9719204.1"
                     /translation="MEVIERQSPATRTQPGLSAKLRQRIATEFQSRWENEWGRKFVTH
                     GVPPGPNAVRLDGNDYLTVTGHPEVVQAQLDSLRRGQEFVIQSGVFQLDGNPASQLQN
                     ALASWLGKEDGLLCQSGYTANLGLLQVIADPQTPVYLDSLAHMSLWEGARAAGAPAYG
                     FRHNDPAHLSKMATRHGPGLVVVDSVYSTTGAVARLPELVEVAQQHDCMILVDESHSL
                     GTHGPGGKGLCAMLGLTDSVHFITASLAKAFAGRAGFFTMPAELRPYLMCHSYPSIFS
                     SCLLPHEVAGLAATMEVIKRGDEARRQLHANTRRVRAAFEESGYPIHQGTEQIISLEA
                     GPESDTLALRDALESRGVFGAVFCAPATSRNRAMVRLTLNASLTDAEMSHVAAVAREI
                     APLVKPWDWPIAKRQATRSA"
     gene            complement(6751..>9282)
                     /gene="secA"
                     /locus_tag="VGA59_05695"
     CDS             complement(6751..>9282)
                     /gene="secA"
                     /locus_tag="VGA59_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX9719205.1"
                     /translation="LRHYDVQLIGGMVLHSGSIAEMKTGEGKTLVATLPVYLNALTGK
                     GVHVVTVNDYLANRDAQWMGKLYSFLGMTVGTNLPNMQREEKQAAYGSDITYGTNNEY
                     GFDYLRDNMVYEVADRVQRGLNYAIVDEVDSILIDEARTPLIISGQAEDHTDLYIAIN
                     RVVPLLSKQEGEADPRTGEGIIKPGDFTVDEKTHQVFLTEQGHENAERILFNLGLIPE
                     GATLYDPANITLMHHLYAALRANHLYHRDQHYVIQEGEVVIVDEFTGRLMSGRRWSDG
                     LHQAVEAKEGVKIQAENQTLASITFQNFFRLYGKLAGMTGTADTEAYEFQEIYGLETV
                     VIPFNKPSRREDQLDRVYKTTREKYEAAIKDIRECYQRGQPVLVGTTSIENSEVIDEL
                     LNKEGLPHQVLNAKQHAREAEIVAQAGRPKMITIATNMAGRGTDIVLGGNVEKAIEAM
                     EADESLDAAAKEHKAAQMRAQWQTDHEFVVAQGGLRIIATERHESRRIDNQLRGRSGR
                     QGDPGSSRFYLSLDDPLMRIFAGERVKAIMERLKMPDGEAIEAGIVSRSIESAQRKVE
                     ARNFDIRKQLLEYDDVANDQRKVIYQQRNDILDAKDLSAQIASLREGCFTDLVHQYVP
                     PESVEEQWDVPGLERVLADEWAIQLSLQQEVQSASSITDEDLLEKVLVVANTAFDAKV
                     QQVGTENFTQFERIVLLQSIDSHWREHLSALDYLRQGIHLRGYAQKQPKQEYKREAFE
                     LFGQLLDSVKNEVTKILMTVSVQSGEQLEQAADELEDRAERISNVTYSAPTETGDVET
                     KLDERTARQIASAAIASAAMPRVGRNEPCPCGSGKKYKHCHGKLA"
BASE COUNT         1432 a         3120 c         3044 g         1686 t
ORIGIN      
        1 gttgaagcgc gcttcgttca aacaaccacg acgagtcaga gcacgatgcg cgctggcgcg
       61 cgcggccacg cgtgagagca aacgcgaggc gctccgtgtg atcgatgcgc cgcggcccgc
      121 ccggccccgc ctttgcagga gacgctgctg gcctgcggcg cgcacaccac cagcgcgtcc
      181 acgcggcagg cggtgcccgg cgggggcgat ctctggggcg gctgtgatgc tcggcctggg
      241 ggcgggcgcg cttgcgcgct tcgtaaactg actcgtcgcg gttgtttgaa cggagggccc
      301 ttcaaggccc gcagtgagtt ctgcgacgcc gaccccggac cgagcagcgc agcgaagtcg
      361 gagcgaagcg cagaccgcca cagtgtgagc ccccgccggg caccgcctgc cgcgaccccc
      421 tgatggccgc agcgtcagag cttgcgcaga gcgcggattc ctaggacgga atctcgaacc
      481 gccgccgcgc gatttccagc agcccgccga agatgaggct ttccaccagc tcgaaagcga
      541 ccgacatgtg gggcgccatc ttccagcccg cgccgccggt catgaagcag gccggttcgg
      601 ccccgcagtg ccggcgcaca tgctgcacca tccgttcgat cgccccggcg atggcatagg
      661 taccgccgct ggtcagcgca tcgctggtat tggtcgggaa ctcgcgcaca tcgcccgtgg
      721 gcacgtgcag gccggccgtg cccgactcga gcgcgcgcag catgatgccg tgacccggca
      781 ggatgaagcc gccgaggaac ttgccttgcg cgtccaccgc ttccaccgtg acggccgtgc
      841 cgaccatcac gacgaccagc ggccggggcg cgccgcgggc cagcatgcgg tgccgggcac
      901 cgatcatggc gacccagcgg tccgctccca gccgcgcggg atggtcgtat ccattgacca
      961 ggcccccttc ggcggcgttg ggcaccaccc agtgcgcagg tacatcccac agctcctcca
     1021 tctgctcctc gacccgccgc ttcacggcat cggcggccac ggcgcagccc agcatgcgct
     1081 cgggcggccg cagggccgac caggcgccgt ccgccaactt gtcgatgttc tcgaggaatt
     1141 cggcaccgtg cgcgcgcagg gtggcgccgg ggctgggggc ctcgtacagc gcccacttca
     1201 gccgggtgtt tccgacatcg accgccagaa agcccatggt cacctctcgc cggaacgcct
     1261 ggcggcggcg gatgaccccc cggccggcat gatcgtcatg cggcctccag ctgggttccg
     1321 agcgccgtga cgctttccgc cagggcttct tcctggacct gcagctgccc gcagtcggcg
     1381 accgcctgtt tgacgctcag ctggagttcg cgatgggctt ccatgggacc gtccatgttc
     1441 agcttgggcg aactgccgtc ggcggcggcg gccgccagcg cggacacgct ttcctgccag
     1501 gccttgacgc ccgacgcgag cgcctgctgc agcagtttca gcagcgccga gcgccgggcc
     1561 gccgcgcctt gcccctgctg gtgcgtccgc tgggccgcgg agctcaccgc gcgcagcgtc
     1621 ttgagccgcg ccacgaggat ttcgcaacgg gcctcgtctt ccttgatctg ctcctgggcc
     1681 gccgcgtcgg ccggcgcggc ctgccgggac ttgagctgat tgcgcatgtc ctgcagccag
     1741 cgcgcgccct ggtcgatgat cttgtcgatc gcgtggtatt cgacttcgac ttccagcagc
     1801 gcgaaatcga ccgccgtggc ctgctccttc tgcttttttt gcagcgcctg cgtctggccc
     1861 gccagcgcgc gagcgaccag gtcggtccgg ttgaactgcg cggcgaattc agcgccggcg
     1921 ctgcggctct tgcccgtggt gcgagcccac cagccgcggt cctgcagcag gccgaaatcg
     1981 agcccgggca aggcatgcgc caggcgcccc agttccgaaa ccgcaggcca atccacccgc
     2041 agcagggcct gcgcctgaag ctcgccgtgc agttcgcgca ggcggcgcac atgcggatgg
     2101 ccggcgtcgg cggccatccg gctgagccat gccgccggtg aaaccgcggt cctgggctgg
     2161 atctgcgcca gcttggacgg cgtcatcagg gaggcggtgc gatcgtgatc ggccattgca
     2221 gcggttgggg tgatcaaccg caatataaac taaacacttt cgcggccgcc agcgtcaaat
     2281 ggcgcccagc cagcaggcgc gctggatagc cgccatcttg ttgtcgacct tgagcttgcc
     2341 catggcgttg gccaagtggt aattgaccgt gcgctcgcta cagtgcagcc gcaccgaact
     2401 ttcgcgcgcg gtgaggccct ggaaagcgag ggccaggcac tcgcgctcgc gcgggctcag
     2461 ggtggaacgg gcgttcgcga cgcagcgctt gaccagcggc cagatgttca aggccgacca
     2521 cacgagcgcg gccgcttcgg agcggtcggt gagttcgcgc gggctgaaca tgaagcactc
     2581 gaaggcacgg cccgccggca gcgaaaactc cacccgcacc acggtctgga aaccgtgggc
     2641 aagccacagc aggcgccagc ggctcgagct gagatggtcg gacttcgcga tgtattgcca
     2701 ggccacgagc ggcgcatcgg agtcgcgcca gggcgcgccg aagtcgcggc tttccgccag
     2761 cgccttggcc gcatccgcca ccaaaggggg atgcacggcc accacctgcc ggtcctcgct
     2821 ggtgccgaaa gggtccggtc ccagaaccgc cacggcctcc accgaaaact cgcgcagtgc
     2881 accgacccag tcgctcaaga ggccgtccag gctcacactg tcaaagttaa caggtacccc
     2941 catggtcttg tcttttcgta tctgatgggg ctgagacaat atcatcatcc actgccgttg
     3001 atggcagccg aggggcgcac ctgatgtgag ggacttactt gtcgtcgata cgtgtgctgt
     3061 acagcggcgc tgccgaactg ctgaagtcgt gggcgtcgaa cgaggtgatg cggcccctgc
     3121 tggagccgat cctccatccg tcgcgatggc gcatccgcgc gctcggcctg tccaccgtgt
     3181 tcggccatcc cctcttctac tggatctggg ccaagctgct gccgcagccc tacgaaaacc
     3241 tggggctgcg cctgctgatg agcggcctgg gcgtgagcct gctcgttttt cccagcatca
     3301 ccgcgagccc gcccagcaag ttcgcggccg ccgtgttcac ggttattttc tggatcacgc
     3361 tgccctggtt tttctcgtgg atgtacttct gcaacaacgg caatcccgtc tggctggcca
     3421 gcctggccgc catgttcctg atctactacc atctcaccga ctggcgcctg gcaacgctgg
     3481 gcacgacgac cgggatgatg gccgcatgga tcctgtttcg cgcggtcggg ccgcaaacgc
     3541 ccgggctgtc ggtcgaagag gccgcaacca atggcgtggt catcgccttc agctggggca
     3601 tgggcgtggt cctgggaatc tcgtcctcca acctgcggcg ggaacaactg gactacacgt
     3661 tgggcacgat gggcatcatg gcccacgagc tgcgcacgcc gctggcgacc atgtcgctga
     3721 tcggcgacgc cgtgcgcggc gccgcgtcgc gatgcgaatc cgatccgcgg caaacgctcg
     3781 aacagctcgc gagccggctg cacgcgctgg tgcgcaacat gaatcaccag atcgacacgc
     3841 agatcgccaa tgcccggctg atgcgcctgc cggtccacaa ggaggcggtc agcgccgccg
     3901 agttggtgcg cgaagccgtc aatacctatc cctatcgcag ttcgcgcgag cgggaatgcg
     3961 tgaagcttca ggtccaaaac gacttcctgt tcgagggatc tcatgccctg ttcctgcagg
     4021 tggtcgacaa cctgctgaag aacgccttgc gctcactggc ggccgccatc gccgccagcc
     4081 agcccgggga catcacgatc gaggtcggta cggcgggcaa tcgcggccgc attgtcgtca
     4141 cggaccgcgg ggtcggcatg gatgcggacc tgcaggcgcg aatcttcgag cccttctttt
     4201 cgaccgatcg cggctccggc cacggtttgg gcctggcttt ctgccagcgc gtgatcgacg
     4261 gcgccaacgg ctccatccgc gtcaagtcgg agcccggcca cggcgcttca ttcacggtgg
     4321 aactgcccat cctcgcctga caccatgaac aacaaccctc cgctccccct acccggaccc
     4381 ataccatgag ctttcccctg atccatcgcc cgggcaccgt ggtctttctc gatgacgatc
     4441 ccgactacct cgagatgctc gccctggtgc tgccgcgcca ttggcacgtc aaactgttcg
     4501 tgcgtcccgt cgaatgcatc aattacctcc agcaggagcc gccgttctgg gaagccgacg
     4561 cgtggaacca gcagcagttg atcgaccact ggcgcgaggg caagcggctg atcccgcaag
     4621 tcctgggcta ctggtcgaag tacacggagc gctatgccct gacgcgcgtg tgtgtcttcg
     4681 actattcgat gcccggcatg gacgggttgc aggcgctggg cgaactggtg gattggccgg
     4741 gttcgcgggt cctgctcacg gggcaggccg acgagcaggt tgcggtacgg gccttcaacc
     4801 gcggcctgat cgatcaattc atcgccaagc agacgcccga catctcgcgc cgcctgatcg
     4861 aagccgtcga gcacctgctg gcgacgccca atgcccgtca tgcgcaaacg tggcgggtga
     4921 cgctcgggcc cgagcagaac gcgctgctgc gcatgccttc cgtgggacgt gacctcgcgg
     4981 gcttcgcagc caagcgctgg gtcgaacatg tcgtgatcgg cgacccgttc ggcgtcctgg
     5041 gaatggacgc cgccggcaac gtcagttggc tgcagctcga gacgcccgag gggctcaagg
     5101 cacaggccga actggccgaa ctcgggggcg tgccgccgtc gggcctggac gacatccggc
     5161 agggcctcaa gctcgcggat ctcgaactgc gccaggggct cggccgcacg ggtccgcccg
     5221 aactggccgc cgctttcccc gtgggccagg atggggccgt gctgggcgcg ctgttcgcgg
     5281 tcgactcgcc gctttgcccc gaccccggca acagctacag caactggctg gcacggcaga
     5341 gcaagcggca tgttcaggac tagggcctga ttcaggctga gcgcgtcgcc tggcgcttcg
     5401 cgatgggcca gtcccagggc ttgaccaggg gcgcgatttc cctggctacc gcggccacat
     5461 gcgacatctc cgcgtcggtc agcgaagcat tgagggtcaa gcgcaccatc gcgcggttgc
     5521 ggctggtcgc cggcgcgcag aacacggcgc cgaacacgcc ccgcgattcc agcgcatcgc
     5581 gcagcgccag ggtgtcgctt tccggcccgg cctccagcga gatgatctgc tcggtgccct
     5641 ggtgaatcgg atagcccgat tcctcgaacg cggcacgcac gcggcgggtg ttcgcgtgaa
     5701 gctgccggcg cgcctcgtcg ccgcgcttga tgacttccat cgtcgccgcc agcccggcaa
     5761 cctcgtgggg cagcaggcac gagctgaaga tgctcggata gctgtggcac atcaggtagg
     5821 ggcgcaattc ggcgggcatg gtgaagaagc cggcgcggcc cgcgaaagcc ttcgcgaggc
     5881 tggccgtgat gaagtgcaca ctgtccgtga ggcccagcat cgcgcacagc cccttgccgc
     5941 ccggaccatg cgtgcccagc gaatgggact cgtccaccag gatcatgcag tcatgctgct
     6001 gcgccacctc caccagttcg ggcagacgcg cgaccgcgcc ggtggtgctg tagaccgagt
     6061 ccaccaccac cagtccgggg ccatggcgag tcgccatttt cgacaggtgc gcggggtcgt
     6121 tgtggcgaaa accgtaagcc ggcgctcccg cggcgcgcgc accctcccac agcgacatgt
     6181 gggccagact gtcaagatag acaggggtct gcggatcggc aatcacctgc agcaagccca
     6241 ggttggccgt gtagcccgac tggcacagca gtccgtcttc cttgcccagc cacgaagcca
     6301 gggcattctg cagctgcgag gcggggttgc cgtcgagttg gaacacgccg gattgaatga
     6361 cgaactcctg gccgcggcgc aatgagtcca gctgggcttg caccacctcg ggatggccgg
     6421 tgacggtgag gtagtcgttg ccgtccaggc gcactgcatt gggccccggg ggaacgccgt
     6481 gcgtgacgaa ttttcggccc cattcgtttt cccagcgcga ctggaactca gtggcaatgc
     6541 gttgccgcag cttggccgac aggccgggct gggttcgtgt ggccggcgat tgccgttcga
     6601 tcacttccat ggaaactcct tgagagagct ccattgaagg ggtagttcag ctgttgcggc
     6661 tagtaccccc tgtcagatct gacagggtta gttaccaatg cttactcata agtgtcggca
     6721 gacacacccg atgccggaaa ccggaggtgc tcaggccagt tttccgtggc agtgcttgta
     6781 tttcttgccg cttccgcaag ggcagggctc gttgcggcca acgcgcggca tggcggccga
     6841 ggcgatggcg gccgaggcga tctggcgcgc cgtcctttcg tcgagcttgg tctcgacatc
     6901 gcccgtttcg gtcggggcgc tgtaggtcac gttggaaatg cgctcggcgc ggtcttccag
     6961 ctcgtcggcg gcctgttcca gctgctcgcc cgactgcacc gacacggtca tcaggatctt
     7021 ggtgacttcg ttcttcacgg aatcgagcag ctgcccgaac agctcgaagg cctcgcgctt
     7081 gtattcctgc ttcggctgct tttgcgcgta gccgcgcagg tggatgccct ggcgcaggta
     7141 gtcgagcgcg gacagatgct cgcgccagtg ggaatcgatg ctctgcagca gcacgatgcg
     7201 ctcgaactgg gtgaaattct cggtgccgac ctgctgcacc ttggcgtcga acgcggtgtt
     7261 ggcgaccacc agcaccttct ccagcaggtc ctcgtcggtg atgctgctgg ccgattgcac
     7321 ttcctgctgc agggacagct ggatcgccca ctcgtcggcc agcactcttt ccaggccggg
     7381 aacgtcccac tgctcttcca ccgactccgg cggcacatac tggtggacca ggtcggtgaa
     7441 gcagccttcg cgcagcgagg cgatctgggc cgacaggtcc ttggcgtcga gaatgtcgtt
     7501 gcgctgctgg tagatcacct tgcgctggtc gttggccacg tcgtcgtatt cgagcagctg
     7561 cttgcggatg tcgaagttgc gcgcctcgac cttgcgctgc gcgctctcga tgctgcggct
     7621 gacgatgccg gcttcgatcg cctcgccgtc gggcatcttc agccgctcca tgatcgcctt
     7681 gacgcgctcg cccgcgaaga ttcgcatcag cgggtcgtcc aggctcaggt agaagcgcga
     7741 cgagccgggg tctccctgtc ggcccgaacg gccgcgcagc tggttgtcga tgcggcggga
     7801 ctcgtggcgt tcggtggcga tgatgcgcag gccgccttgc gccaccacga actcatggtc
     7861 cgtctgccac tgggcgcgca tctgcgccgc cttgtgttcc ttggcggccg catccagcga
     7921 ctcgtcggct tccatcgcct cgatcgcctt ttccacattg ccgcccagca cgatgtccgt
     7981 gccgcggccg gccatgttgg tggcgatggt gatcattttc ggccgccccg cctgggccac
     8041 gatctcggcc tcgcgcgcgt gctgcttggc gttgagcacc tggtgcggca ggccttcctt
     8101 gttcaacaac tcgtcgatca cctccgagtt ctcgatcgat gtcgtgccca ccagcacagg
     8161 ctgcccgcgc tggtagcact cgcgtatgtc cttgatcgcg gcctcgtatt tctcgcgggt
     8221 cgtcttgtag acccgatcga gctggtcctc acgccggctg ggcttgttga acgggatcac
     8281 caccgtctcc aggccgtaga tctcctggaa ctcgtaggct tcggtgtcgg ccgtgccggt
     8341 catgccggcc agcttgccgt acagccggaa aaagttctgg aaggtgatcg aggccagggt
     8401 ctggttctcg gcctggatct tcacgccttc cttggcttcc accgcctggt gcaagccatc
     8461 gctccagcgg cgcccggaca tcaggcggcc ggtgaattcg tcgacgatga cgacctcgcc
     8521 ctcctggatc acatagtgct ggtcgcggtg atacaggtgg ttggcccgca gcgccgcgta
     8581 caagtggtgc atcagcgtga tgttggccgg gtcgtagagc gtcgcgcctt cgggaatcag
     8641 gcccaggttg aacaggatgc gctcggcgtt ctcgtggccc tgctcggtga ggaatacctg
     8701 gtgggttttt tcgtccaccg tgaagtcgcc gggcttgatg atgccttcgc ccgtgcgcgg
     8761 gtcggcctcg ccttcctgct tggacagcag cggcacgacg cggttgatcg cgatgtacag
     8821 gtcggtgtgg tcctcggcct gcccgctgat gatcagcggc gtgcgcgctt cgtcgatgag
     8881 gatcgagtcc acttcgtcga cgatggcata gttcagcccg cgctggaccc ggtccgccac
     8941 ttcgtagacc atgttgtcgc gcaggtagtc gaagccgtac tcgttgttgg tgccgtaggt
     9001 gatgtccgag ccgtaggccg cctgcttctc ctcgcgctgc atgttcggca ggttggtccc
     9061 gaccgtcatg ccgagaaagc tgtagagctt gcccatccac tgcgcgtcgc ggttcgccag
     9121 gtaatcgttc acggtgacga catggacgcc cttgcctgtg agggcattga ggtaaaccgg
     9181 cagggtcgcc accagcgtct tgccttcgcc cgtcttcatt tcggcgatgg agccgctgtg
     9241 gagcaccatg ccgccgatga gttgcacgtc gtagtgacgc ag
//
LOCUS       DASWIY010000150         6108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_455806, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000150 DASWIY010000000
VERSION     DASWIY010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455806"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..2014)
                     /locus_tag="VGA59_05700"
     CDS             complement(44..2014)
                     /locus_tag="VGA59_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9719206.1"
                     /translation="MSRPGEQKLKHLFQPGQFGKFKTKNMVKYGACCVSNYNTRDGFI
                     TPRELARTRVIAGTGCGIITNQGAYPDPLGEGKSYFRQVALFDDKFIPQFEMIAGYIH
                     DNGGVAIQQILHAGRYGGIDLGYCIQPSVVPQTLPHFRPPREVTKEQIRAIVGQHADA
                     ARRAIRAGFDGTEVTSFMGYLLANFNSRFTNQRTDEYGGSIENRGRFMRELIDAIKQA
                     TPGNPLVIRLNGAELMDRWGGNSEDECFELMQQAVDVGVDMISVTVGWQEAPESSIGR
                     DVAPGHWNYLSAKAKKLFPNTLITFGNRLPDPVMANECIKDGVFDFWEVCRPLLADPE
                     LIHKAADDRLDEVRRCIGSLNCLSRLFRDLPYTCTMNPTLGHEVEPEYQITPAAVKKK
                     VMVIGAGPAGMEAAITAKKRGHDVTVFEKSDRIGGNLRAYADNDLARPDDLLSVVRHY
                     EVMAQKLGIEMRFNTEANPKFMRSILHQYDVCLIAAGARTDMKAYRHVKGCELLVDAL
                     DVAHGRVMPAKRLVMIGGGKIGLTLAESLTKAGHEVVIVEEDKRIAGDVIPSFKWRHS
                     AWVEELEIRTLTSTHLVAVTAEGATVKNAKGEETFVPTDMVLVSGPRLANHDLFQEFQ
                     WMIDELHGAGDAVIPRGMDAAIHEGYRMGVRI"
     gene            complement(2052..2270)
                     /locus_tag="VGA59_05705"
     CDS             complement(2052..2270)
                     /locus_tag="VGA59_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719207.1"
                     /translation="MIFSNPSGAPELACDQCGCRWFDRMTNTCYECGAPVTPENLAEI
                     EAALAAFAASKAANDATNPGPGATPPQP"
     gene            complement(2267..2737)
                     /locus_tag="VGA59_05710"
     CDS             complement(2267..2737)
                     /locus_tag="VGA59_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719208.1"
                     /translation="MTTTTETSAFGDIRDWQAMLERHAPLFTELVPGSAVGFVPRAGS
                     GIHPGKHVAGVMNAGGEGEMLTWAMEGQSMRAGVRHYSGFQDARVDLLFVADEAGLAA
                     MREALHGETLSIIKRQIRKGAIMFFVMKTKFELQDAGYEDFLDSLGLAFLGACR"
     gene            complement(2734..>6108)
                     /locus_tag="VGA59_05715"
     CDS             complement(2734..>6108)
                     /locus_tag="VGA59_05715"
                     /inference="COORDINATES: protein motif:HMM:NF009588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX9719209.1"
                     /translation="RGSPLGGLDTALNEARPFLAAHDIRFHPAVNEDLAATAIWGTQQ
                     LHLMPSPRRDGIFGMWYGKGPGVDRCGDVFKHANFAGTSPHGGVLVVAGDDHSARSSA
                     VAHQSEHVFSACAIPVLAPAGVQDILDLGLHGWALSRYAGCWVAMKTMSDTAESSAAV
                     EVDRDRVTIAIPEDFALPKDGVHLRWPDPPLAQERRMQEFKIYAAVAYARANGLNRLV
                     FDTPRPRLGIIACGKAWLDVRQALADLGIDERVAGDIGLRLYKVGMPWPLEAAGARRF
                     ARGLEEILVVEEKRQIIEYQLKEMLYDWPDSERPRVVGKFDESGEWPAPPHQWLLPPT
                     GDLSPAIVARVIAGRIAHYHESEAIREHLAVLLEQERTMAGYRVPLQRTPYFCSGCPH
                     NQSTRVPTGSCAVAGVGCHLMAVGMERNTLTISQMGGEGVTWLGMAEHSGTQHVFANM
                     GDGTYFHSGLIAIRAAIAAGANITYKLLFNHAVAMTGGQPIDGSLTVPQLTRQLEAEG
                     VRRIVVLADDTGKYPAADRFAPGVDIRPREDLQAVQRELREIPGVTILVYDQTCAAEQ
                     RRRRKRDGAAQPLRRLFINEAVCEGCGDCGVKSNCLSVVPVATEFGTKRAIDQFSCNL
                     DESCLAGFCPSFVSVEGATLRRPRAAVDLADVPGADIAGPPEPILPSLEAPYGLLIAG
                     VGGTGVVTIGALVGMAAHLEGKGVTVLDMTGMSQKAGAVTSHVRIARHPDELHAARIA
                     TAQADAVLGCDIVVASGADARSTMRRGRTRALVNTAQAITGEFVRHPDQAFPLPAMQQ
                     SLSDAIGADALELVDATRLASLLLGHSIATNMFMLGFAWQRGWVPVSRAALMRSIELN
                     GVAVPDNQLAFEWGRRAAWDPEGTECVAANAESVPSARRLSQGLDEVIARRREILVAY
                     QNDAFARRYAELVERVRRAETRAVAGSTRLAEIVARNSFKLMACKDEYEVARLFGDAD
                     FRRALHAGFEGDVKVRLHLAGPMFSRPDPATGEARKRSYGPWILPVLRLLSRLKVLRG
                     TPFDIFGSTAERRLERELSADYARTIGALVEDLHAGNHELACEIAGIPETIRGFGPVK
                     QRNAAAARERQRELMSAWTQCCGPAGDEPKPLESLN"
BASE COUNT          958 a         2054 c         2051 g         1045 t
ORIGIN      
        1 cggatccggg atgacagcca tgagcgagac ttgtgatgcg ctctcagatc cgcacgccca
       61 tccggtagcc ttcgtggatg gcggcgtcca tgccgcgcgg gatcaccgcg tcaccggcgc
      121 cgtgcagctc gtcgatcatc cactggaact cctggaacag gtcatggttg gccaggcgcg
      181 gacctgagac cagcaccatg tcggtgggca cgaaggtctc ctcgcccttg gcgttcttca
      241 cggtcgcccc ttcggccgtg accgcgacca gatgggtcga ggtcagggtt ctgatctcca
      301 gctcctcgac ccaggccgaa tgccgccact tgaaggacgg aatcacatcg ccggcgatgc
      361 gcttgtcctc ctccacgatc accacctcgt ggccggcctt ggtcagcgac tcggccagcg
      421 tgaggccgat cttgccgccg ccgatcatca ccagccgctt ggcaggcatc acccgcccat
      481 gggccacgtc cagggcatcc accagcagct cgcatccctt tacgtggcgg taggccttca
      541 tgtcggtgcg ggcgccggcc gcgatcaggc acacatcgta ttggtgcagg atgctgcgca
      601 tgaacttggg attggcctcg gtattgaagc gcatttcgat gccgagtttt tgcgccatca
      661 cctcgtagtg gcgcacgacg ctgagcaggt cgtccggtcg ggccagatcg ttgtcggcgt
      721 aggcgcgcag gttgccgccg atgcggtcgc tcttctcgaa caccgtcacg tcgtggccgc
      781 gcttcttcgc cgtgatcgcc gcttccatgc cggccggacc ggcgcctatg accatcacct
      841 tcttcttcac ggccgccggc gtgatctggt actccggctc gacctcgtgg cccagggtcg
      901 ggttcatggt gcaggtgtag ggcaggtcgc ggaacaggcg cgacaggcag ttgagcgatc
      961 cgatgcagcg gcgcacttcg tccagccggt cgtcggcggc cttgtggatc agctcgggat
     1021 cggccagcag cggccggcag acctcccaga aatcgaacac gccatccttg atgcactcgt
     1081 tcgccatgac gggatcgggc aggcggttgc cgaaggtgat caaggtgttg gggaagagct
     1141 tcttggcctt ggccgacagg tagttccagt gccccggcgc cacgtcgcgg ccgatcgagg
     1201 actcgggcgc ttcctgccag cccacggtga ccgagatcat gtccacgccc acgtcgaccg
     1261 cctgctgcat cagctcgaag cattcgtcct cgctgttgcc gccccagcgg tccatcagct
     1321 ccgcgccgtt gaggcggatc accagcgggt tgccgggtgt ggcctgcttg atggcgtcga
     1381 tcaattcgcg catgaagcgg ccgcggtttt cgatcgagcc gccgtattcg tcggtgcgct
     1441 ggttggtgaa gcgcgagttg aagttggcca gcaggtaacc catgaagctg gtgacttcgg
     1501 tgccgtcgaa accggcgcgg atggcacgcc gggcggcgtc ggcatgttgc ccgacgatcg
     1561 cccggatctg ctccttggtg acttcgcgcg gcggacgaaa atgcggcagc gtctggggta
     1621 ccaccgaggg ctggatgcag taacccaggt cgatgccgcc gtagcgcccc gcgtgcagga
     1681 tctgctggat cgccacgccg ccgttgtcgt ggatgtaccc cgcgatcatc tcgaactgcg
     1741 ggatgaactt gtcgtcgaac agcgcgacct ggcggaagta ggacttgcct tcgccgagcg
     1801 gatcgggata ggcgccctgg ttggtgatga tgccgcagcc ggtgccggca atgacgcgcg
     1861 tgcgtgccag ctcgcgcggc gtgatgaagc cgtcgcgggt gttgtagttc gagacgcagc
     1921 aggccccgta cttgaccatg ttcttggtct tgaacttgcc gaactggccg ggttggaaca
     1981 ggtgtttcag tttttgctca ccggggcggg acatggaaag ctcctcgaat cgatcggtta
     2041 aaaagaaaag gtcagggctg gggcggcgta gcgcctgggc ccgggttggt tgcgtcgttg
     2101 gcggccttgc tcgccgcgaa tgcggccagc gccgcttcga tctcggccag gttctcgggc
     2161 gtgacggggg cgccgcactc gtagcaggtg ttggtcatgc ggtcgaacca gcggcagccg
     2221 cattggtcgc acgcgagttc gggcgcgccg ctggggttgc tgaagatcat cgacaagccc
     2281 ccaggaaggc caggccgagc gagtcgagaa agtcctcgta gcccgcgtcc tggagttcga
     2341 acttggtctt catcacgaaa aacatgatcg cgcccttgcg gatctgccgc ttgatgattg
     2401 acagggtttc gccgtgcaag gcctcgcgca tcgccgccag cccggcctcg tcggccacga
     2461 acagcaggtc cacgcgcgcg tcctgaaagc ccgagtagtg cctcacgccg gcgcgcatgc
     2521 tctggccctc catcgcccag gtcagcatct cgccttcgcc gccggcattc atcacgccgg
     2581 ccacgtgctt gcccggatga atgcccgatc ccgcgcgcgg cacgaagccg acggcggagc
     2641 cgggcacgag ctcggtgaac agcggcgcgt gacgctccag catcgcctgc cagtcacgga
     2701 tgtcgccaaa ggcgctggtt tcggtggtgg tggtcaattc aggctttcaa gcggtttggg
     2761 ctcgtcaccc gccgggccgc aacattgcgt ccatgcactc atcagctcgc gctgacgctc
     2821 gcgcgcggca gccgcgttgc gctgcttgac gggtccgaag ccacggatgg tttcgggaat
     2881 cccggcgatc tcgcaggcca gctcgtggtt gccggcgtgc aggtcctcga ccagcgcgcc
     2941 gatcgtgcgt gcgtagtccg cgctcagctc gcgttccagc cggcgctcgg cggtactgcc
     3001 gaagatatcg aatggcgttc cgcgcaatac cttcagcctg gacaacaggc gaagcacggg
     3061 caggatccag ggcccataag agcgcttgcg agcctcgccg gtcgccgggt cgggccggct
     3121 gaacatgggg cccgccaggt gcagccggac cttgacgtcg ccttcgaacc cggcgtgcag
     3181 ggcgcgccgg aaatcggcat cgccgaacag tcgcgccacc tcgtactcgt ccttgcaggc
     3241 catcaacttg aacgagttgc gcgccacgat ttcggcgagg cgcgtgctgc ccgccaccgc
     3301 acgcgtctcc gcgcggcgca cccgctcgac gagttcggcg tagcgccgcg caaatgcgtc
     3361 gttctggtac gccacaagaa tctcgcgacg gcgggcgatg acttcatcca gcccctgcga
     3421 cagccggcgc gcggagggca ccgactcggc gttcgccgcc acgcattcag tgccttcagg
     3481 atcccaagcc gcccggcggc cccactcgaa agcgagctgg ttgtcgggca cggcgacgcc
     3541 attgagctcg atcgatcgca tcagtgccgc gcgcgaaacc ggtacccagc cgcgttgcca
     3601 ggcaaatccc agcatgaaca tgttggtggc gatcgagtgc ccgagcaaca acgacgccag
     3661 gcgcgtcgca tcgaccagtt caagggcgtc ggctccgatg gcgtcgctca gcgactgctg
     3721 catcgccggc agcggaaagg cctgatcggg gtgccggacg aactcgccgg tgatggcttg
     3781 agccgtgttg accagcgcgc gggtgcggcc gcgccgcatc gtcgaacgcg cgtcggcgcc
     3841 ggacgccacg acgatgtcgc atcccagcac ggcatcggcc tgggcggtgg cgatgcgcgc
     3901 ggcatgcagc tcgtcgggat ggcgggcgat gcgcacgtgc gaagtcacgg ccccggcctt
     3961 ttgagacatg cccgtcatgt cgagcacggt gacgcctttg ccctccagat gggcggccat
     4021 gccgaccagc gcgcctatcg tcaccacacc ggtgccgccg acgccggcga tcagcagccc
     4081 gtacggtgcc tccagcgacg gcaggatcgg ctcgggaggg cccgcgatat cggcacctgg
     4141 cacgtccgcc aggtcgacgg ccgcgcgggg ccgccgcagt gtcgcaccct cgacactgac
     4201 gaagctgggg caaaagccgg ccaggcagct ctcgtccagg ttgcaggaga actggtcgat
     4261 cgcgcgtttg gtgccgaact cggtggccac cggcacgacc gacaggcagt tggacttcac
     4321 gccgcaatcg ccgcagccct cgcagaccgc ttcattgatg aacaatcgcc gcagcggctg
     4381 cgccgcgccg tcgcgcttgc ggcgccgacg ctgttccgcg gcgcaggtct ggtcgtacac
     4441 cagtatggtg acgccgggta tctcgcgcag ctcgcgctga acggcctgca ggtcttcgcg
     4501 cgggcgaatg tcgacgcccg gtgcaaaacg gtcggccgcg ggatactttc cggtgtcgtc
     4561 cgccagcacc acgatgcggc gcacgccttc ggcttcgagc tggcgcgtga gctggggcac
     4621 cgtcaacgag ccgtcgatcg gctggccacc tgtcatggcc acggcatggt tgaagagcaa
     4681 tttgtacgtg atgttggcgc cggcggcaat tgcggcgcga atcgcgatga gcccggagtg
     4741 gaagtacgtg ccgtcgccca tgttggcaaa cacgtgctgc gtgccggagt gttccgccat
     4801 gcccagccag gtcacgccct cgcctcccat ctggctgatc gtgagcgtgt tgcgctccat
     4861 gccgaccgcc atcaggtggc agcccacgcc agccaccgcg cacgagccgg tgggcacgcg
     4921 ggtggactgg ttgtgcgggc agccggagca gaaatacggc gtgcgctgca gcggcacgcg
     4981 gtatcctgcc atggtgcgtt cctgctccag cagcacggcc aggtgctcgc ggatagcctc
     5041 gctctcgtgg tagtgcgcga tgcgcccggc gatcacgcgc gccacgatgg ccggcgacaa
     5101 atctcctgtg ggcggcagca gccattgatg cggcggcgcg ggccattcgc cgctctcatc
     5161 gaactttccg accacgcgcg gccgctcgct gtccggccag tcgtagagca tctccttcaa
     5221 ctggtactcg atgatctggc gcttctcctc gaccaccagg atttcttcca ggccacgcgc
     5281 gaagcggcgc gcgccggcgg cctccagcgg ccatggcatg ccgaccttgt acaaacgcag
     5341 cccgatgtcg ccggcgacgc gctcgtcgat ccccaggtcg gccagcgcct ggcgcacatc
     5401 gagccaggcc ttgccgcagg cgatgatccc caggcgcggc cggggcgtgt cgaacaccag
     5461 ccggttcagc ccgttggcgc gcgcgtaagc caccgcggca tagatcttga actcctgcat
     5521 ccggcgctcc tgagccagcg gcggatcggg ccagcgcagg tgcacgccgt ccttcggcag
     5581 cgcgaagtct tcagggatcg cgatcgtgac acggtcgcgg tccacttcga ccgcggccga
     5641 actctcggct gtgtcggaca tggtcttcat cgccacccaa cagcccgcgt aacgcgacag
     5701 ggcccagccg tgcagcccga ggtcgagaat gtcctgcacg ccggccggcg ccagcaccgg
     5761 aatggcgcaa gccgagaaaa cgtgctcgct ctggtgcgcg acggccgatg aacgggcgct
     5821 gtggtcgtcg cccgcgacaa ccagcacgcc gccgtgcggc gatgtgcccg cgaagttggc
     5881 gtgcttgaac acgtcgccgc aacggtccac gccgggcccc ttgccgtacc acatgccgaa
     5941 gatgccgtcg cgccgggggc ttggcatcag gtgcaattgc tgcgtccccc agatggcggt
     6001 ggccgcgagg tcctcgttaa ccgcgggatg gaagcggatg tcgtgtgccg ccaggaaggg
     6061 ccgcgcctcg ttcagcgccg tgtccagtcc acccagtggc gagccgcg
//
LOCUS       DASWIY010000151         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_83342, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000151 DASWIY010000000
VERSION     DASWIY010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83342"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VGA59_05720"
     CDS             complement(<1..473)
                     /locus_tag="VGA59_05720"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX9719210.1"
                     /translation="MAVFPQGKSRVRERANAGEKRVLDALARHLEDDYAVWHNIPIMG
                     SGREPDFVILHPQRGLLILEVKDWKRQTIAQANTMLVALRTPHGLTNTEHPVSQARGY
                     MLDLVHQLQHHPDLLHKDGQHKGKLLMPWGWGAVLSHIKAAEVAGDTSFFEVFEP"
     gene            complement(782..1414)
                     /gene="eda"
                     /locus_tag="VGA59_05725"
     CDS             complement(782..1414)
                     /gene="eda"
                     /locus_tag="VGA59_05725"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX9719211.1"
                     /translation="MNILELMRIGTVIPVIVIEKIEHAVPLARALVAGGLRVLELTLR
                     TPVALQAIGAIAQEVEGAIIGVGTITRADDFAESIKAGALFGVSPGLTHELIAAARDS
                     GLPLLPGVMTPSDVIAARTAGFTALKLFPAQQAGGVSMLRALGGPFPDVTFCPTGGIT
                     AATAPDFLRLPNVACVGGSWLTPPDAIAVGDWGRITALARETVQLRRQSG"
     gene            complement(1411..>1984)
                     /locus_tag="VGA59_05730"
     CDS             complement(1411..>1984)
                     /locus_tag="VGA59_05730"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX9719212.1"
                     /translation="HRVVRAPAIVFDNQEAVMAAFDRGELQRDFVAVVRYQGPRANGM
                     PELHKLTPLLGVLQDQGFQVALVTDGRMSGASGKVPAAIHVTPECLAGGALARVRKGD
                     MILLDAETGVLQAEVADAVWQARQAEAVDLSQHHHGMGRELFAAFRANALGAEEGAVT
                     FNAPEVQGTGFSAPAVPIHDRFVFANGAAT"
BASE COUNT          352 a          663 c          637 g          332 t
ORIGIN      
        1 tgcggctcga acacctcgaa aaagctggtg tccccagcca cctccgccgc tttgatgtga
       61 ctgagtacgg caccccagcc ccagggcatc agcaacttgc ccttgtgctg gccgtctttg
      121 tgcagcaggt ccgggtggtg ctgaagctgg tgaaccaggt ccagcatgta gccgcgggcc
      181 tgggaaacgg ggtgttcggt gttggtgagg ccgtggggcg tgcgcaacgc caccagcatc
      241 gtgttggcct gggcgatcgt ctgccgcttc cagtccttca cctcaagaat gagcaacccg
      301 cgctgcgggt ggaggatcac gaagtcaggc tctcggcccg accccatgat gggaatgttg
      361 tgccagacgg cgtagtcgtc ttccagatgc cgtgccagcg catcgagcac gcgcttttcg
      421 cccgcgttgg cgcgctcgcg caccctcgat tttccttgcg gaaatactgc cacggtgagg
      481 cctccctgtc gggagtctag cggagcggcg ggcgccccaa gataggcata ttcctgttta
      541 cccgttggat ggcatcggcc atgggcaagg ccggagggca agccgccaca aaacagaagg
      601 ccgccgccgc tcgagccaac ggccgcctgg gcggcaggcc ccgcaagccc cggccgcttg
      661 aacctgcgtg acaccaccgc ggaagtccgc gctcggtacg agagtggctt ggtatcggtc
      721 atgtaacgac cggtgtgctc gtgctcgcat taaggaggta gatccatggc actgacatgc
      781 gtcaaccgga ctgtcggcgc agctgcactg tctcgcgtgc gagggccgtg atccttcccc
      841 agtcgcccac ggcgatcgca tcgggcggtg tcagccaaga gccgccgaca caggcgacat
      901 tgggcaaccg gagaaaatcg ggcgccgttg ccgccgtgat gccgcccgtg ggacaaaatg
      961 tgacatcggg gaatggaccg cccagagccc ggagcatgct gacgcctccc gcctgctgcg
     1021 ccgggaacag cttcaacgca gtgaagcctg cagttcgcgc agcgatgacg tccgaaggtg
     1081 tcatgacgcc gggcaacagg ggcaagccgc tgtcgcgcgc ggccgcaatc agttcgtgcg
     1141 tgagtccggg actcacgccg aacaaggcac ctgccttgat cgattccgcg aaatcgtcgg
     1201 cgcgtgtgat ggtgcccacg ccgatgatcg ctccctcgac ctcctgggca atggcgccga
     1261 tggcctgcag cgcaacgggc gtgcgcaagg tcagctcgag cacgcgcaaa ccgccggcca
     1321 ccagggctct tgccagcggc accgcatgtt cgatcttttc gatgacgatg accggaatga
     1381 ccgtaccgat gcgcatcaac tccaggatgt tcatgtggca gctccatttg caaagacgaa
     1441 gcggtcatgg atgggaaccg ctggggcgga gaaacccgtc ccttgcacct ccggcgcgtt
     1501 gaaggtgacc gcgccctctt ccgcgccaag cgcgttggcg cggaaggccg cgaacagctc
     1561 gcgccccatc ccatggtggt gctggctcaa gtccacggcc tcggcctgcc gcgcctgcca
     1621 cacggcgtcg gcgacttcgg cctggagcac tcccgtctcg gcgtccagaa gaatcatgtc
     1681 gcccttgcgc acgcgcgcca gcgcaccgcc tgcaaggcat tccggagtca catggatggc
     1741 agccggcacc ttgcccgatg cgcccgacat gcgcccatct gtaaccagtg ccacctgaaa
     1801 accctggtcc tgcaacacgc cgagcagcgg cgtcagcttg tgcagttccg gcatgccgtt
     1861 ggcgcgcggc ccctggtaac ggacaacggc cacgaagtcg cgttgcaact cgccgcggtc
     1921 gaacgcggcc atgaccgctt cctggttgtc gaacacgatc gccggagccc gaaccacgcg
     1981 gtgc
//
LOCUS       DASWIY010000152         2575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_456982, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000152 DASWIY010000000
VERSION     DASWIY010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456982"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1080
                     /locus_tag="VGA59_05735"
     CDS             244..1080
                     /locus_tag="VGA59_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9719213.1"
                     /translation="MTENTGAARIRDPAAVPRLLLTTGIIVVLWPGFRLTEFDLGALF
                     GAHSRETMGKFIATFFPLATSNEFLHLIWKSTLETLAIATAGMTLALLIAFPLSLAVT
                     RSLSISRIGPGRGFLAGRLLRWPMRFMLMFFRSIPEIVWALVFVRAVGLGPAAGVLAI
                     GVTYGGMLGKVYSEILESGDTRPTAALLEAGGRRLGAFLYGMWPIALPELVSYTVYRW
                     ECAVRASVIMGFVGAGGLGQQMELSMRMLNGGEVSSILLVFLLLVVFADAISAFLRRV
                     TA"
     gene            1187..1948
                     /gene="phnE"
                     /locus_tag="VGA59_05740"
     CDS             1187..1948
                     /gene="phnE"
                     /locus_tag="VGA59_05740"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX9719214.1"
                     /translation="MRTVHWIVLAALAAAAWGSFSFLSLDLGQMLTAEARAQMWKYVV
                     SFFPPDFSVEHLARVGYGTLETIAISAIGTALAAFLGLLLALPASGRFGVLARGAARL
                     VLNFLRSIPELVWAALMVMAAGLGPFAGALALALHTTGVIGRLFAETLENAPPDAAQA
                     LQFSGTRSSLAFLYGTMPQVVPQLVAYSLYRWEINIRMAAVLGVVGAGGLGQMLYFSL
                     SLFHQQQAMTVIIAMLVLVVIVDGASAWLRQRLGG"
     gene            complement(1956..>2575)
                     /locus_tag="VGA59_05745"
     CDS             complement(1956..>2575)
                     /locus_tag="VGA59_05745"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9719215.1"
                     /translation="PTVIQRFIQRYPKVRLSLREGSPQQISELMQSGEADIAIITEAY
                     EVYRDLVLLPCSQWNRCVITPLRHPLLAERQLTLDAIARFPLVTYDFAFSGDSPIRRA
                     FDARGLQPDVALTAVDADVIKAYVELGMGVGILAKMAFDPARDFGLRLIDASHLFEAS
                     TTHIGIRRHAWLRGYVYDFIEMFAPHLTRSVVEKTMRGEGSDYEL"
BASE COUNT          391 a          735 c          884 g          565 t
ORIGIN      
        1 ctaggagttt gtcggactta gccccgacaa actccttatg caaaaccggt gccgggagaa
       61 gtgcgagaaa atgatgaaaa ctatgcgatg tcacccacgc ttttcctcaa attcgatcgc
      121 tttggctcgg tttattatga attgtcagtc ggttttgctt atagcagcct gtgcggaccg
      181 ctgagtccga caggctgcca ggcgcggtga tgagcgggcg agtgcaccac tgaagggtga
      241 agcgtgacgg agaataccgg ggccgcgcga attcgggatc ccgctgcggt gccgcggctg
      301 ctcctgacga ctggcatcat cgtcgtgctt tggcccggtt tcaggctcac ggaattcgat
      361 cttggtgcgt tgtttggtgc gcacagccgc gagacgatgg gcaagttcat cgccaccttt
      421 tttccgctcg ccacctccaa tgagtttctt cacctgatct ggaaatccac gctcgaaacg
      481 ctggccattg ccactgccgg catgacgttg gcgctgctga ttgcattccc gctttcgctg
      541 gcggtgaccc gcagcctgtc gatttcccgc atcggccctg ggcgcggctt tctcgccggc
      601 cgtctgctgc ggtggccgat gcgtttcatg ctgatgtttt ttcgcagcat tcccgagatc
      661 gtatgggcgc tggtgttcgt gcgcgcggtt ggtctcggcc ctgccgctgg ggtgctggcg
      721 atcggcgtga cctacggcgg catgctgggg aaggtctatt cggagattct cgaatcgggc
      781 gatacacgtc ctacggcagc gctgctcgaa gccggcggcc ggcggctggg ggcgttcctg
      841 tacggcatgt ggccaattgc gctgccggaa ctcgtttctt ataccgttta ccggtgggaa
      901 tgcgcggtac gtgcgtcggt catcatggga ttcgtcggcg ccggcggcct gggtcagcag
      961 atggagttgt cgatgcgcat gctcaacggc ggcgaggtca gcagcatatt gctggtcttc
     1021 ctgctccttg tcgttttcgc agacgccatc agcgcattcc tgcgcagggt gacggcatga
     1081 gcaagccggc gaggggcccc ttgagggggt cgacggcaac gcgaattgcc gtcaggcgcc
     1141 gacgcgaaga tggtcgacgt gttgtgggac ggggagaggc gacggcatga gaacggtgca
     1201 ctggatcgtg ctggccgcgc tggctgcggc ggcctggggc agtttttcgt ttctgtcgct
     1261 ggatttgggg cagatgttga cggccgaggc gcgcgcgcag atgtggaaat acgtcgtgtc
     1321 gttctttcca cccgatttct ctgtggagca cctggcgcgc gttggctatg gcacactgga
     1381 aaccatcgcg atttccgcga tcggcactgc gttggcggcg tttctgggat tgctgctggc
     1441 cttgcccgcc tcgggccgtt ttggggttct tgcgcgtggc gcagcccgcc ttgtgctcaa
     1501 tttcctgcgt tcaatcccag agctggtctg ggcggcactg atggtgatgg ctgcggggct
     1561 gggtcctttc gccggcgcgc tcgcgctggc gctgcacacc acgggcgtga tcgggcggct
     1621 gttcgcggag acgctggaga atgcgccgcc cgacgcggcg caggccttgc agttttcggg
     1681 cacccggtca tcgctcgcct ttctctacgg gacgatgccc caagtcgtgc cgcaactggt
     1741 tgcgtattcg ctctaccgct gggagatcaa catccgcatg gccgccgtgc tcggtgtggt
     1801 tggggccggc ggcttgggtc agatgctcta tttttcgctg agcctttttc accagcagca
     1861 ggcgatgacg gtgatcatcg caatgctggt gttggtggtg atcgtcgacg gcgccagcgc
     1921 ctggctgcgc cagcgcctgg gaggttgatg tcttcctaga gctcgtagtc cgagccttcg
     1981 ccgcgcatgg ttttctccac cacgctgcgg gtgaggtgcg gcgcgaacat ctcgatgaaa
     2041 tcgtagacat agccgcgcag ccaggcgtgg cggcggattc cgatgtgggt cgtgctggcc
     2101 tcgaagaggt ggctcgcgtc gatcagccgc aatccgaagt cgcgcgccgg atcgaacgcc
     2161 atcttggcga ggatgccgac ccccatgccg agttcgacgt aggccttgat gacgtcggcg
     2221 tccacggcgg tgagcgcgac gtcgggttgg aggccgcgcg cgtcgaaggc gcggcgaatc
     2281 ggagaatcgc cgctgaaggc gaagtcatag gtcaccagtg gaaagcgtgc gatcgcgtcg
     2341 agcgtgagct gtctttccgc gagcagcggg tggcgcagcg gcgtgatcac gcagcggttc
     2401 cactgcgaac acggcaacag caccaggtcg cgatagacct cgtaagcctc ggtgatgatc
     2461 gcgatatcgg cttcgcccga ttgcatcagt tccgaaatct gctgggggct gccttcgcgc
     2521 agcgacaggc ggaccttggg atagcgctgg atgaagcgct ggatcacggt gggca
//
LOCUS       DASWIY010000153        10058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3401153, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000153 DASWIY010000000
VERSION     DASWIY010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10058
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3401153"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1300)
                     /locus_tag="VGA59_05750"
     CDS             complement(122..1300)
                     /locus_tag="VGA59_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9719216.1"
                     /translation="MFSLASIAPVKYEDPWNLPLRDRVRSLARGRQRVAYFYEKADNS
                     TFRYRVYNMVHVLNSGGNATSAAYFFLADLHRLEEIAELADVLVICRTRYDHRVNHLV
                     GAFRRRGKRVLFDTDDLVFNTEYAHLLVKTLDLDITNPQVWDDWFAYTSRLGATLKLC
                     DGAVTTNDYLAAQIAGFAGVPTAVVPNFINPEQLELSDRVFEAKRALSPLANDEIHLG
                     YFSGSPSHNRDFAMVSAALEEALEEDPRLAVVVVGYIEAGPGLTRFGNRVVRFPFHDW
                     VNLQRLVGAVEFNLMPLQHNVFTHCKSELKYFEAAVVGTQSIASPTYTYARAIRHGHN
                     GYLAQAHQWLSAIRQAVSDLERYQSVAERTRTDAREKYAWFNQRACVFAALGLEDLTA
                     "
     gene            complement(1300..2781)
                     /locus_tag="VGA59_05755"
     CDS             complement(1300..2781)
                     /locus_tag="VGA59_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719217.1"
                     /translation="MSPIEAIRRARRYWGTHGTRQLARESVARLVRKILGPSYAAGGL
                     PMADNPTRSMSLIETIRRARRYWRIHGTRQFLRESLARLARKVLGPSYTVAGGLPVSD
                     EHGWVSVGDLARKQAQMCAPLRLFSVPTSEPPRVSVVTDSINRGSLYGGVGTAIILAA
                     LVAEASGARLRIVTRTERAQPTGLETVLNTYGISLSHDVEFVFAPFYDTAYEIDFFER
                     ELFITTSWWTTAATLGSVRSESIVYLLQEDERMFYPHGDEHLRCSRILANRDIRFVIN
                     TRLLFEHLVASGLPNIADRGTWFEPSFPHEVFHPRERADGAKRTLMFYARPNNVRNLF
                     FFGIEILEAAIARGIIDLQQWDLLLVGKDIPKLRFDDGRYSPQVQENLSWTQYAALAG
                     QVDLALCLMYTPHPSYPPFDLAASGAVVVTNRFGNKQDLGGYSRNIICGDTDLESMLE
                     AMAQGARLATDAHQRGENYRANRLGSDWTRSLADVVRQVSGMH"
     gene            complement(2816..4600)
                     /locus_tag="VGA59_05760"
     CDS             complement(2816..4600)
                     /locus_tag="VGA59_05760"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX9719218.1"
                     /translation="MLQESDLVPYQAVTEIPAHGVLVLAPHADDEVFGCGGAIAAHVR
                     SGVPVTVIVLTDGAAGHGEPSQRAQESRAAAQVLGCAAPEFWAYPDRGLRCEDEVIQR
                     VTEALAACGADLLYAPSPWEIHPDHRQAAAIAAEAVRRTGIRLAYYEIGAALRPNLLL
                     DITALAETKARAMQCFGSQLERQDYERQIRALNEYRSYSLPHEVLAAEAYLLLSAADV
                     DTALVAGLTALPVSLGGSPSAAAAQPEGALVSILIRSLDREHLQEALDSVALQTYPNI
                     EVVVIAAQPDHRELPRRCGRFPLRLVPTQEALTRSRAANKALAQARGDYLLFLDDDDW
                     LMPGHIARLAHVLQRQPNTQAVYTGVALAAEGGGPLGQVVDLPFDGVRQLAGNLTPIQ
                     AVLFRKELPARGCRFDESLDRFEDWDFWLQVARHAPMVHLPGVSAAYRIHQSSGVHED
                     SGPLGAATQVIYRKWQASWSAGQGAALMRQAWSCADLEVELASARESLSALHDLLSEH
                     ARTINRLVQDAGEQSQVIAQHIQLIAQQSERIAQQTQLIAQQTQAAEAQERLAEERQR
                     QIAELLQSTSWRVTAPLRWLSSKLRPRA"
     gene            complement(4627..5994)
                     /locus_tag="VGA59_05765"
     CDS             complement(4627..5994)
                     /locus_tag="VGA59_05765"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEX9719219.1"
                     /translation="MDIRARMTRAWASRLGAWLLVLATAIGLSTLIPPIQSPDEHSHI
                     RRAYLLSKGEITLVAPPGTSSGGSVDAALLSFIDTYLLTIAISAQTPPSAAQRAEAES
                     LRWGGPERFVPVPGTGYYMPLVYVPHAAALLAGRVLGLTIADSYRLVRLASLMSCLGL
                     LAAAWRVMRPPPLAIALLLLPMTLFQLLAPTLDGLTTCLAVLALSVFSDGVLQEKDWP
                     GERSWLLAASVALLATSRIQLLPLLGLPFFIAWTRRSRRDAFLGSAAVLGSMAWVAYA
                     LANTVDLRVQRGDSTGHLLLQYAADPVAFLRVVAASLADSELATLYTHSFIGVLGWLD
                     TWLPLPAYPALWTGLGLCAVASVLGTNLRQAGLARTFLALAALACALLVFLALLVTWT
                     PHPAATVAGVQGRYFVVPAIMLAYAIGATAPPRRLPRPTLALLAAVAAVSLYALVATL
                     LARYH"
     gene            complement(5973..6323)
                     /locus_tag="VGA59_05770"
     CDS             complement(5973..6323)
                     /locus_tag="VGA59_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9719220.1"
                     /translation="MSIQNAVLLALNVLALSVGQVLFKLAAAGFDGSEPFVRQLLFNK
                     YLIAALVVYGLATGLWVALLRQVPLHVAYPFVALAFFFVPLMAHWVLDEPLRWQTLAG
                     ASVIFVGVWISGLE"
     gene            complement(6337..7296)
                     /locus_tag="VGA59_05775"
     CDS             complement(6337..7296)
                     /locus_tag="VGA59_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9719221.1"
                     /translation="MQSNIAVIIPCYRVKAHILGVIARVGSEVTRIYVVDDACPEGTG
                     EFVQAQCRDPRVTVLRNERNLGVGGAVMAGYAAALRDGANILVKVDGDGQMDPALIPR
                     FVRPIAMGQADYSKGNRFYDLTRIGQMPALRLVGNAALSFMAKLSTGYWNLFDTTNGF
                     TAIHARVARRIPFAKVSQRYFFETDMLFRLNIIRAVVADIPMDASYGSEQSNLKIRKV
                     VWEFVAKHVRNFTKRVLYNYFLRDMTVASLELVFGAGLLLFGLVFGSYQWLKAVSNSM
                     ATPLGTIMLAALPTLLGLQMLLAFVGFDVANVPRRPIHPDLPD"
     gene            7347..8405
                     /locus_tag="VGA59_05780"
     CDS             7347..8405
                     /locus_tag="VGA59_05780"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX9719222.1"
                     /translation="MPPQRHDDPAIEALRGLAALIVVAAHYARFVTDQTGAWGFASTG
                     VDLFFVLSGYVFAPYFFGKQLTLGSHLVRRFFRLYPLYVLALLTYAALKMPDPSAWSH
                     FWTHLFMAHTLQSMEIASFYNPAFWSLPPEVEFYLVLPLLAWALGRRGFGWLFTGALV
                     LHLALAAMAVPGEGATARAIATVHLPGLLIEFCLGAWASKLARERASGPAAALRLAGG
                     AVWLVTMGAVYATLIAGGRDQSAPGWISGNIGLGAAVGYALVVSALGTRADGLRDWRL
                     RACLVAGQLSYGVYLFHNAAPPILARLWPGAAGWTALLASLALTLLLAFALHHAVEAP
                     VRDFGRELSRRIGPRVPA"
     gene            complement(8696..9793)
                     /locus_tag="VGA59_05785"
     CDS             complement(8696..9793)
                     /locus_tag="VGA59_05785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9719223.1"
                     /translation="MSSPPCVDIAIVNYFSAPDVRRCLERLGPWTHGTVWLVDNSLDA
                     AEFAALRECTQALPWVRLIDAGGNLGFGRACNLAFEQSTSPLFLLLNPDARATPADLL
                     ALAQALDSDPRLGAVSPRIYWNDARTFLLPEAFAQTPWTALAQALASRTQRLTRWAAA
                     RYLAGQRQRMASQSAFDVAFLAGAVMMLRREAVLAAGGLFDPDYFMFFEDTDLSLRLR
                     RSGRRLAMVPAAAAVHEYRHKPFKAGMMARSRSQYYLKRYAWFHRLSGGLTRLDALVR
                     PVPLQRWFEPLPMACRTLADFTAQTHARGVVAFSPSMVMMPAIFRPAGETVRGFDEDE
                     WALLEPAEYVALMQGADAAAPSRWVHFQRVA"
     gene            complement(9771..>10058)
                     /locus_tag="VGA59_05790"
     CDS             complement(9771..>10058)
                     /locus_tag="VGA59_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719224.1"
                     /translation="VPNVGHWSVVRDLALGRFDYGPVGILCGTHLRFFTARSLERLLG
                     EAGFEVVRWRRAGPPMPEEFGRFLAATAQAQLAWDTESLRTESLHVLATLR"
BASE COUNT         1707 a         3329 c         3431 g         1591 t
ORIGIN      
        1 caggacttgc tgcggctgcc cgcgaaaatc gtcctcagcc tctggctggc cgaggaccgg
       61 gccgcggcgg cgaaggcgct ggtgcgcgga gttgccgacg ggcttgcgaa ccgcggcggt
      121 gtcatgccgt gagatcctcg agcccgaggg cggcgaacac gcacgcgcgc tggttgaacc
      181 acgcgtattt ttcccgcgcg tcggtccggg tccgttcggc caccgactga taccgctcca
      241 ggtcgctcac ggcctggcga atggcgctga gccactggtg agcctgcgcc agatagccgt
      301 tgtgcccgtg gcggatggcg cgcgcgtacg tgtaggtggg tgaggcgatg ctttgcgttc
      361 ccaccaccgc ggcttcgaaa tacttcagct ccgacttgca atgggtgaag acgttgtgct
      421 gcagcggcat caggttgaac tccaccgcgc ccaccaggcg ttgcaggttc acccagtcgt
      481 ggaacggaaa gcgcacgacg cgattgccga atcgcgtcag gcccgggccg gcttcgatat
      541 agcccaccac caccaccgcc agccggggat cctcctcgag cgcttcctcc agcgccgcgg
      601 ataccatcgc gaagtcgcgg ttgtgcgaag gcgatccgct gaaatagcca aggtggatct
      661 catcgttggc caggggggac aaagcccgct tggcttcgaa cacccgatcc gacagctcca
      721 gttgctcggg gttgatgaag ttcggcacca cggcggtggg cacgccggca aacccggcga
      781 tctgcgcggc gaggtagtcg ttcgtggtca ccgcgccgtc gcacaatttc agtgtcgcgc
      841 ccaggcggct ggtgtaagcg aaccagtcgt cccagacctg gggattggtg atgtccaggt
      901 cgagcgtctt gaccagcagg tgcgcgtact ccgtgttgaa aaccaggtca tccgtatcga
      961 acagcacgcg cttgccgcgc cttcgaaaag cccccaccag atggttgacg cggtggtcgt
     1021 agcgggtgcg gcagatcacc agcacatcgg ccagttcggc gatttcctcc agccggtgaa
     1081 gatcggcgag aaagaaataa gcggccgagg ttgcgttgcc gcccgagttg aggacatgca
     1141 ccatgttgta gacgcggtac ctgaaggtgc tgttgtcggc cttttcgtag aaataggcga
     1201 cgcgctgccg cccccgcgcg agcgagcgca cccgatcgcg cagcggcagg ttccaggggt
     1261 cctcgtattt gaccggggcg atcgacgcga gcgaaaacat cagtgcatac cgctgacttg
     1321 ccgaaccacg tcggccagcg accgcgtcca atcggaaccc agccggttgg cgcggtagtt
     1381 ctcgccgcgc tgatgcgcgt cggtggccag ccgtgcaccc tgggccatgg cctcgagcat
     1441 cgattccaga tcggtgtcgc cgcagatgat gttcctgctg tagccaccca gatcctgctt
     1501 gttgccgaag cggttggtga ctaccacggc cccgctggcc gcaaggtcga aaggcggata
     1561 gctcgggtgc ggcgtgtaca tgaggcacag cgccaggtcg acctggccgg ccagcgccgc
     1621 gtactgtgtc caactgaggt tctcctgcac ctgcgggctg tagcggccgt cgtcgaacct
     1681 gagcttcggg atgtccttgc cgaccaggag aagatcccac tgctgcaggt cgatgattcc
     1741 acgggcgatc gcggcttcga ggatttcgat accgaagaaa aagaggttgc gcacattgtt
     1801 cggcctcgca tagaacatca gcgtccgctt ggcgccgtcc gcgcgctccc gcggatggaa
     1861 cacttcgtgc ggaaacgagg gttcgaacca ggtgcctcga tcggcgatgt tcggcaggcc
     1921 cgaggcgacg aggtgctcga acagcagccg cgtgttgatc acgaagcgaa tgtcccggtt
     1981 ggccaggatt ctcgagcagc gcagatgctc gtcgccatgc ggatagaaca tgcgctcgtc
     2041 ctcctgcagc aggtagacga tggactcgct gcgcacgctg cccagcgtcg cggccgtggt
     2101 ccaccaggac gtcgtgatga agagctcgcg ttcgaagaag tcgatctcgt aggcggtgtc
     2161 gtaaaaaggc gcgaagacga actccacgtc gtgggaaagg ctgatgccgt aggtgttcag
     2221 cacggtttcc aggccggtgg gctgtgcccg ctcggtgcgc gtcacgatcc tcagccgggc
     2281 gccggaagcc tcggccacca gcgccgccag gatgatggcg gtgccgacac cgccataaag
     2341 gctgccgcgg ttgatgctgt cggttaccac gctcacccgc gggggctcgc ttgtgggtac
     2401 cgagaagagc ctgagcggcg cgcacatctg ggcctgcttg cgggccagat cgcccacgct
     2461 cacccatccg tgctcgtcgg acacaggcaa gccgccggcg accgtgtagc tggggcccag
     2521 taccttgcgg gcgaggcgcg ccagcgactc gcgcaggaac tgccgggtgc catggattcg
     2581 ccagtagcgc cgcgctcggc gaatcgtttc gataagggac atggatcgtg tagggttgtc
     2641 ggccatcggc aggccgcccg cggcatagct ggggccaagt atctttcgga ccagccgcgc
     2701 caccgactcg cgcgccagtt gccgcgtgcc gtgcgttccc cagtaacgcc gcgctcggcg
     2761 aatcgcttcg ataggggaca tgggtcgcgt tggattcctg cgttacgaat gagctttagg
     2821 ccctgggcct gagcttggaa gacagccagc gcaagggggc ggtgacccgc cagctcgtgc
     2881 tttgcagcaa ctcggcgatc tgcctctggc gctcttcggc caggcgttcc tgggcctcgg
     2941 cggcctgcgt ctgctgggcg atcaactgag tctgctgggc gatccgctcg gactgctggg
     3001 cgatcagctg gatgtgctgg gcgatcactt gcgactgctc cccggcatcc tgcaccagcc
     3061 ggttgatcgt tcgcgcatgt tcgctcagca ggtcgtgcag agcgctcagg ctctcccgtg
     3121 cggaggccag ttcgacctcg aggtcggcac atgaccaggc ctgccgcatc agcgccgcac
     3181 cttgtccggc cgaccaggag gcctgccatt tcctgtagat cacttgcgtc gccgcgccca
     3241 gcgggccgct gtcttcatgc actccggagc tctggtggat ccgataggcc gcgctcacgc
     3301 ccggcaggtg gaccatgggc gcgtggcgcg ccacctgcag ccagaagtcc caatcttcga
     3361 atcgatccag cgactcgtcg aaacggcatc cgcgggccgg cagctccttg cggaacagca
     3421 ccgcctgaat gggtgtgagg ttgcccgcga gctgccggac gccgtcgaac ggcagatcga
     3481 ccacctggcc gagcgggcca ccgccttctg cggcgagggc cacgcccgtg tagacggcct
     3541 gcgtgttcgg ctgccgctgc aacacgtgcg ccaggcgcgc aatgtggccg ggcatcagcc
     3601 aatcgtcgtc gtcgagaaac agcaggtagt cgccccgggc ttgcgccagt gccttgttcg
     3661 ccgcccggct gcgcgtcagc gcctcctggg taggcaccag gcgcaatgga aagcgcccgc
     3721 agcgccgggg caattcccga tgatcgggct gtgcggcgat caccaccacc tcgatgttgg
     3781 gataggtctg cagtgccacc gaatcgagcg cttcctgcag atgctcgcgg tccaggctgc
     3841 gtatgagaat cgaaaccagc gccccttcag gctgcgcggc cgctgccgag ggactgcctc
     3901 ccagcgaaac aggcagcgcc gtgaggccgg cgaccagtgc cgtgtcgacg tcggcggcgg
     3961 acagcagcag atacgcctcg gccgccagca cttcgtgcgg cagcgaataa ctgcggtact
     4021 cgttcaatgc gcggatctgc cgttcgtaat cctgccgttc cagctgcgag ccgaagcact
     4081 gcatggcgcg cgctttcgtc tcggccaagg cggtgatgtc cagcagcaga ttggggcgca
     4141 gggccgcccc gatttcgtaa taggcgaggc gaatgccggt cctgcgcacg gcctcggccg
     4201 caatcgctgc agcctggcgg tgatcgggat ggatttccca gggcgacggc gcatacagca
     4261 ggtcggcacc gcaagcggcc agggcctcgg tgacccgctg tatcacttcg tcttcgcagc
     4321 gcagtccgcg gtccgggtag gcccagaact cgggcgctgc gcaaccgagc acctgcgccg
     4381 ccgcgcggct ctcctgcgcg cgttgggagg gctcgccgtg gcccgcggcc ccatcggtga
     4441 gcacgatcac cgtgacggga acgccggagc gcacatgcgc cgcgatggcc ccaccgcatc
     4501 caaacacctc gtcgtccgca tgcggcgcca gaaccagaac accgtgagcg ggaatctcgg
     4561 tgacggcctg atagggaacg agatcgcttt cttggagcac gcagacgtcc gatggttgca
     4621 aaaccatcaa tgatagcggg cgagtagggt ggcaaccaag gcgtagagcg agacggccgc
     4681 cacggccgcg agcagggcca gggtgggccg gggcaggcgg cgaggcggcg ctgtggcgcc
     4741 gatggcatag gccagcatga tcgccggcac cacgaagtag cgtccctgca cccctgcgac
     4801 cgtggcggcg gggtggggag tccaggtcac gagcagcgcc agaaaaacca gcagggcaca
     4861 ggcgagcgcc gccagtgcga ggaaggtgcg ggcaagcccc gcctggcgca agttcgtgcc
     4921 cagcaccgaa gccacggcgc acaggcccag gccggtccaa agggccgggt acgcgggcaa
     4981 cggcagccag gtgtcgagcc agcccagcac gccgatgaac gagtgggtgt aaagagtggc
     5041 gagctcggag tccgccaagg aggcggcaac cacgcgcaaa aaggccactg ggtcggccgc
     5101 gtactgcagc agcaggtgtc ccgtgctgtc accgcgctgc acccgcagat cgaccgtatt
     5161 cgccagcgca taggccaccc acgccatcga gccgaggacg gccgcgctgc ccagaaaggc
     5221 atcgcgccgt gaacggcgcg tccaggcgat gaaaaacggc aggcccagca gcggcagcaa
     5281 ctggatccgg ctcgttgcca gcaacgcgac gctggcggcg agcagccacg aacgctcgcc
     5341 gggccagtct ttttcctgca gcacgccatc cgaaaacaca ctgagggcca gcacagccag
     5401 gcaggtcgtc aggccatcca gcgtaggcgc cagaagctgg aacaaggtca tcggcagcag
     5461 cagcagcgcg atggcaaggg ggggcggccg catgacgcgc cacgccgccg ccagcagacc
     5521 gaggcacgac atcagggacg ccagccgcac cagccggtaa gagtcggcga tcgtcaggcc
     5581 gaggacgcgg ccggccagca gtgccgccgc gtgcgggacg tagaccaacg gcatgtagta
     5641 ccccgtgccc ggtaccggaa cgaagcgctc gggcccgccc caacgcagcg attccgcctc
     5701 ggcacgctga gccgcgctcg gcggcgtctg cgcgctgatg gctatcgtca gcaagtaggt
     5761 gtcgatgaag gacaacagcg ccgcgtcgac gctcccgccc gagctcgtgc ccggcggggc
     5821 gaccagggtg atctcgcctt tggagaggag gtaggcgcgc cggatgtggc tgtgctcgtc
     5881 gggcgactgg atgggcggga tgagtgtgct cagcccaata gctgtcgcga gcacgagcag
     5941 ccacgccccc agcctggaag cccaggcgcg tgtcattcga gcccggatat ccacacgccg
     6001 acgaagatca cggacgcacc cgccagcgtc tgccagcgca gcggctcgtc cagcacccaa
     6061 tgcgccatga gcggcacgaa gaaaaatgcc agtgcgacga acgggtaagc cacatgcagg
     6121 gggacctggc gcagcagtgc cacccacagg cccgtggcca gtccgtacac gaccagagcg
     6181 gcgatcaggt acttgttgaa cagcagttgc cgtacaaacg gttcgctgcc gtcaaagcct
     6241 gccgccgcca gcttgaacag cacctgcccg accgaaaggg caaggacgtt cagggccagg
     6301 agcaccgcgt tctggatcga catgtcgtgg cctgccctaa tccggcagat cggggtggat
     6361 gggacggcgc ggcacgttgg ccacatcgaa gccgacgaag gccagcagca tctgcaggcc
     6421 cagcagggtc ggcaatgccg ccagcatgat ggtgcccagc ggcgtcgcca tcgagttgct
     6481 gacggccttg agccattgat agctgccgaa caccaagccg aacaggagca ggcccgcccc
     6541 gaacaccagc tccaacgagg cgacggtcat gtcccgcagg aaatagttgt acagcacgcg
     6601 cttcgtgaag ttgcgcacgt gcttggccac gaattcccag accaccttgc ggatcttcag
     6661 gttgctttgt tcgctgccgt agcttgcgtc catcgggatg tccgccacca ccgcgcggat
     6721 gatgttcagc cggaacagca tgtcggtctc gaaaaaatag cgctggctga ccttggcgaa
     6781 ggggatgcgc cgcgcgaccc gcgcatggat cgcggtgaag ccgttggtcg tgtcgaacag
     6841 gttccagtac cccgtcgaca gcttggccat gaacgacagc gccgcgttgc cgaccaggcg
     6901 cagggccggc atctggccga tgcgggtcag gtcgtagaag cggttgcctt tgctgtaatc
     6961 cgcctgcccc atcgcgatgg gccgcacgaa tctcggaatc agggccgggt ccatctggcc
     7021 gtcgccgtcc accttgacca ggatgttcgc gccgtcgcgc agtgccgcgg cgtagccggc
     7081 catgacggct ccgccgacgc ccaggttccg ttcgttgcgc agcaccgtca cgcgggggtc
     7141 gcggcattgc gcctggacga attcacccgt gccttccggg caggcgtcgt cgacgacata
     7201 gatgcgcgtg acctcgctgc cgacgcgggc gatcacgccc aggatgtggg ccttgacgcg
     7261 gtaacaggga ataatgacgg cgatgttgct ttgcatggcg gcgcgggagt cccggcgcgc
     7321 cattgtaggc aacaccccga aacacgatgc ccccgcagcg ccacgacgat cccgccatcg
     7381 aagccttgcg cggccttgcc gcgctgatcg tggtcgctgc ccactatgcg cgcttcgtga
     7441 ccgatcagac cggcgcgtgg ggcttcgcga gcaccggcgt cgacctgttc ttcgttctca
     7501 gcggctacgt gttcgcgcca tatttcttcg gcaagcagct gaccctcggg tcccacctgg
     7561 tgcggcgatt cttccgtctc tatccgctct acgtgctggc attgctgacg tatgcggctc
     7621 tcaagatgcc cgatccctcg gcctggagcc atttctggac gcacctgttc atggcgcaca
     7681 cgctgcagtc gatggagatt gcgtccttct acaacccggc gttctggagc ctgccgccgg
     7741 aagtcgagtt ctacctggtg ttgccgctgc ttgcctgggc gctcggaagg cggggcttcg
     7801 gctggctttt caccggcgcg ctggtattgc acctggccct tgccgccatg gccgtgccgg
     7861 gcgagggcgc cactgcgcgc gccatcgcga ccgtgcacct gccgggcctg ctgatcgaat
     7921 tctgcctcgg cgcctgggct tcgaagctcg cgcgcgagcg ggcctcgggc ccggcagcgg
     7981 ccttgcgcct cgcgggcggc gcggtctggc tggtgacgat gggcgcggtg tatgcgaccc
     8041 tgatcgccgg cggccgcgat caatccgcgc cggggtggat cagcggcaac atcggactgg
     8101 gcgccgccgt cggctatgcg ctggtggtca gcgcgctggg cacccgcgcc gatgggctgc
     8161 gcgattggcg gctgcgggcg tgcctggtgg cgggtcagtt gagctacggc gtttacctct
     8221 ttcacaacgc ggccccgcct atcctcgcgc ggctgtggcc gggggcggcc ggatggacgg
     8281 cgctgctcgc gagcctcgcg ctcacgctgc tgctcgcttt cgcgctgcac cacgccgtcg
     8341 aggcgccggt gcgcgatttc ggccgcgagc tcagccggcg gattggccct cgcgtgcccg
     8401 cttagaccag gagcgtgtgc ggcagaaggg ttattgctcc ctctccctct gggagagggt
     8461 gggggtgagg gcaaggtgcc ggcaccgcgc attttcggcc agtcgggatc gggctcgaag
     8521 ggcaaatagc tggctggcat ctgagctcaa cgcattgcac ccacccatcg atgacttgtc
     8581 gcgctcccgc gtgccctcac cccagccctc tcccagaggg agagggagaa acttccatca
     8641 accctctccc agagggagag ggagcgaacc cggcttctgc cgcccacgcg ccttgtcagg
     8701 ccacgcgctg gaaatgcacc cagcgcgacg gtgcggccgc atccgcgcct tgcatcagcg
     8761 ccacgtattc ggccggttcc agcaatgccc attcgtcttc gtcgaagccg cgcaccgttt
     8821 ccccggcagg acggaagatg gccggcatca tgaccatcga ggggctgaag gcgaccacgc
     8881 cgcgtgcgtg cgtctgggct gtgaaatcgg ccagggtgcg gcacgccatg ggaagcggct
     8941 cgaaccatcg ctgcagcggc accggccgca ccagcgcgtc cagccgcgtc aatccgcccg
     9001 agaggcggtg gaaccaggca tagcgcttga ggtagtactg cgagcggctc cgcgccatca
     9061 tgccggcctt gaaaggcttg tgccggtact cgtggaccgc ggctgcggcg ggcaccatgg
     9121 ccagccgccg gcccgagcgg cgcagccgca gggaaaggtc agtgtcctcg aagaacatga
     9181 aatagtccgg gtcgaacagg ccgcccgcgg cgagcacggc ctcgcgccgc agcatcatca
     9241 cggcgccggc caggaaggca acgtcgaacg ccgactgcga ggccatgcgt tgccgctggc
     9301 cggcgaggta gcgcgccgcc gcccatcgcg tgagccgttg cgtgcgcgaa gccagcgcct
     9361 gcgccagggc ggtccacggt gtttgcgcga acgcctccgg caacaggaaa gtgcgcgcat
     9421 cgttccagta gatgcgcggt gagacggcgc ccagtcgcgg gtcgctgtcc agtgcctgcg
     9481 ccagcgccag caggtccgcc ggcgtcgcgc gggcgtcggg gttgagcagc aggaaaagcg
     9541 gcgacgtgga ctgctcgaat gcgaggttgc acgcgcggcc gaacccgagg ttgccgcccg
     9601 cgtcgatcag ccggacccag ggcaatgcct gcgtgcattc gcgcagcgcg gcgaactcgg
     9661 ccgcgtccag gctgttgtcg accagccaaa ctgtgccgtg ggtccacggc cccagccgtt
     9721 ccaggcagcg ccgcacgtcc ggggcgctga agtaattgac gatcgcgatg tcaacgcagg
     9781 gtggcgagga catggaggct ctcggtgcgc aggctctcgg tgtcccaggc gagctgggcc
     9841 tgggcagtcg ccgcgaggaa gcggccgaat tcttccggca tgggggggcc cgcgcgccgc
     9901 cagcgaacca cctcgaagcc cgcctcgccc agcaagcgct ccaggctgcg cgcggtgaaa
     9961 aagcgcaggt gcgtgccgca caggatgccc accggcccgt agtcgaaacg ccccagcgcg
    10021 aggtcgcgca cgaccgacca atggcccacg ttcggcac
//
LOCUS       DASWIY010000154         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2317551, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000154 DASWIY010000000
VERSION     DASWIY010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317551"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VGA59_05795"
     CDS             complement(<1..166)
                     /locus_tag="VGA59_05795"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9719225.1"
                     /translation="MSSSIRKAGAPLQYGSAGNGSPHHLAMEMLQTASGAKFDHIPYR
                     GSQLAIPDLLS"
     gene            490..696
                     /locus_tag="VGA59_05800"
     CDS             490..696
                     /locus_tag="VGA59_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719226.1"
                     /translation="MIDSTQPQFELRFQSLFDSGRGYAFPCDPTGQVDLDSLSERARN
                     NYLYARAMVGRELAVPALRPSDLH"
     gene            complement(745..1122)
                     /locus_tag="VGA59_05805"
     CDS             complement(745..1122)
                     /locus_tag="VGA59_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719227.1"
                     /translation="MDKWLKVSIVAAVLLAGAGVFYGFVFVLPGIERTDQDGGNAGRR
                     ADEFQLAQRRDALEHCRQAARLIYDVHWAVACMSEVGQASSGLADGHAECDLPDAKAA
                     VVNAWLDKAEERCVAEVRAGLDP"
     gene            complement(1275..1880)
                     /locus_tag="VGA59_05810"
     CDS             complement(1275..1880)
                     /locus_tag="VGA59_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719228.1"
                     /translation="MDLFLLAMLVAFSAWALKARDQRRRIALLGSHLAKYQIEKHMET
                     LTQGYLRALGEDDPQRSEPIWNMLRSAEQELGSQFRRFVVDFSRVQEADTRVSKLPVY
                     LPFATKLVDRTFDLRKALAIHARGISQAVEGGGHLSPKAMAFTLSAELFLMQHTCHWF
                     CKSKTVASARVLARHKTSYEQLVAAVAPDTRAAYCALVGRR"
     gene            complement(2007..2423)
                     /locus_tag="VGA59_05815"
     CDS             complement(2007..2423)
                     /locus_tag="VGA59_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX9719229.1"
                     /translation="MSNSQPAGASGLTGGCLCGAVRYEARGAPFHPTVCHCSICRRAA
                     GAPMVAWITVMPADFAFTAGQPSTYRSSAKGQRTHCPICGTQLTFKLDALDEIDVTVC
                     SLDDPEAVPPHDHTYTSTQLSWVHLDDGLPRYAKAR"
BASE COUNT          466 a          890 c          879 g          490 t
ORIGIN      
        1 cgctcagcag gtcgggtatc gcgagttggc tgcccctgta aggaatgtgg tcgaacttcg
       61 ccccgcttgc tgtttgcagc atttccatcg ccaggtggtg cgggctgcca ttgccggccg
      121 agccgtattg cagcggcgca ccggctttgc gtatcgaaga tgaaattgtg ggtaccgggg
      181 cttaccctga gagcttccgc gcgcgcggcg agcctttctc atgtcgccgt accggaccaa
      241 ctgagcgcgc ccgcatcgac tgcctctacg cccgtgcaat ggtcggccgc gagctcggcg
      301 tccccgctgt ggagggcacc gttcacggcc aaccgtccta ctccctgtag gcgcgaacga
      361 gctgctgaga tcggcattgg ccgaggatct ctatcggttt gcgctgatgg tcaaagcttg
      421 acgtgcgccc acactcaaga catgcaagac gtgcgcgagc cagtgacctg cgctcaccgg
      481 agaactcaaa tgatcgattc gacacagccc caattcgaac tgcgcttcca gtccctgttc
      541 gacagcggtc gcggatatgc gtttccttgc gaccccacgg gccaggtcga tctcgatagc
      601 ttgagcgagc gtgctcgcaa caactacctc tacgcccggg cgatggtcgg ccgggagctg
      661 gcagtgcccg cactgcggcc tagcgatctg cattgaggcg gcatcgcttt ttcgcaatcc
      721 cgcgcattgt tcgccaacct tgcgctacgg atcgaggccg gcgcgcacct cggcgacgca
      781 ccgctcttct gccttgtcca gccaggcgtt gacgaccgcg gccttggcgt cgggcaggtc
      841 gcactccgca tggccatcgg cgaggcccga ggacgcctga cccacttcgc tcatgcaggc
      901 cacggcccag tggacgtcgt agatcagccg cgccgcctgc cggcagtgct cgagggcatc
      961 gcgccgctgc gcgagctgga attcgtccgc tcgccgcccg gcgtttcccc catcctgatc
     1021 ggtccgctcg atcccgggca gcacgaagac gaatccgtag aacaccccgg cgccggcgag
     1081 cagcaccgcc gccacgatgg ataccttcag ccacttgtcc atgctgagcc acgaagggag
     1141 cggggatcac cataacacgg catcagggcg ccgtgtcagg ccgtaggtga ctgacagttt
     1201 cgcgccagcc tcgggccggc cgaatttcgg cggagtgttc ctgctactcc aaaggctggg
     1261 ctatgacgag cgcgctaccg ccgcccgacc agcgcgcaat aggccgcccg cgtatcgggg
     1321 gcgacggccg cgacaagctg ctcgtaggac gtcttgtgcc gggccagcac gcgcgccgag
     1381 gcgacggtct tggatttgca gaaccagtgg caggtgtgct gcatcaagaa cagctcggcc
     1441 gacagggtga aagccatcgc tttgggcgac aggtgtccgc caccctccac tgcctggctg
     1501 attccacgcg cgtggatggc caatgctttg cgcaggtcga aggtgcgatc cacaagcttc
     1561 gttgcaaacg ggaggtagac cggcagcttg ctcacgcgcg tgtcggcctc ctggacgcgg
     1621 gagaagtcga caacgaagcg ccggaactgg ctgcccagct cctgttcggc ggaccgtagc
     1681 atgttccaga tcggctcgct gcgttgcgga tcgtcttcgc caagcgcgcg caggtagcct
     1741 tgcgtcagcg tctccatgtg cttttcgatc tggtacttcg caagatggct gcctaacagc
     1801 gcgatgcggc ggcgctggtc ccgggccttg agcgcccagg cggaaaaggc gacaagcatc
     1861 gccagcaaaa acaagtccat tgcggcggtc tggttcaggg gaaggtgggg cgagtgtagc
     1921 caccacgctt tgccggggtc cgggatgaca cacgattccg gaatgacgaa cggcccacca
     1981 caagatgcgc agcgcgccgg gcgtgctcaa cgcgccttcg cgtaccgcgg caatccatcg
     2041 tccagatgca cccagctcag ctgcgtcgat gtgtaggtgt ggtcgtgcgg cggcacggct
     2101 tcggggtcat ccaggctgca gaccgtcacg tcgatttcgt ccagcgcatc cagcttgaag
     2161 gtgagctgcg tgccgcatat cgggcagtgg gtgcgctggc cttttgcgct ggagcggtag
     2221 gtgctgggct gccccgccgt aaaggcaaag tccgccggca tcaccgtgat ccaggccacc
     2281 atgggcgcac cggccgcgcg gcggcagatg ctgcagtggc agaccgtggg atggaacggc
     2341 gcaccgcgcg cttcgtagcg gacggcgccg cagaggcatc cgccggtgag gccgcttgcg
     2401 ccggccggtt gggagttgga catcgcagcc ttcctttcat ccggcatgtt tcgacggggt
     2461 gagttgcaca cggtagcacc ggggcggcct cgcccggatt gcccccgaaa aactcggccg
     2521 cctgaaacgc agctcagcag tggccacgaa gcggggttcc ccttgttacg cgtcagcgtg
     2581 ttacataacg gcgtttgctg ttacacgtag ccacgctccg ttgcgaaacc tgatatatgt
     2641 ctgctgagta atgctttccc ccaacgaccc aaggagcacc ccatgaagca acttcgcaaa
     2701 cccattgcgg cggccctggc ggccg
//
LOCUS       DASWIY010000155         7284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2318356, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000155 DASWIY010000000
VERSION     DASWIY010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2318356"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..837
                     /locus_tag="VGA59_05820"
     CDS             19..837
                     /locus_tag="VGA59_05820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9719230.1"
                     /translation="MNQNIQAHYARPDIGKQILAALEKAGKDIDRLTPGDLAPIDEFH
                     IRGRAATLELARAAGVDSTKKVLDVGSGLGGPARALAREFGCQVTGIDLTEEYCRVAE
                     MLSGRIGLSTHLEFLQGDALDLPFADATFDVVWTEHVAMNIPDKSRLYRQMHRVLKPG
                     GTLAIYDILAGPAGPVHFPVPWARVPQSSFLATPDELRLFLDEAGFRIADWADTTDLA
                     RAWFAALAGKVRKEGLPPLGFHLLLGPEFKEMARNQRRNLDEGRIVLAQIVARK"
     gene            1026..1325
                     /locus_tag="VGA59_05825"
     CDS             1026..1325
                     /locus_tag="VGA59_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9719231.1"
                     /translation="MEIAMTNLTPLETELVALGAALGSNCVPCIEHHIPQARKAGLSD
                     LQIRAVIELADKVRQVPARKVLETAVRLLGEESGAQAGGMERECASMGGAASKCC"
     gene            1574..1723
                     /locus_tag="VGA59_05830"
     CDS             1574..1723
                     /locus_tag="VGA59_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719232.1"
                     /translation="MKPRITMITLGTDDLEAAGYAGYFQDPDGHLWEVAWNPQMPPQD
                     EGRLI"
     gene            1720..2559
                     /locus_tag="VGA59_05835"
     CDS             1720..2559
                     /locus_tag="VGA59_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9719233.1"
                     /translation="MTKAYVHGYDPREALRLQDQATTLVELLHSDTAYPAGHTVLEAG
                     CGVGAQTVTLARNSPNASITSIDLSETSLAKARAAVQAAGVTNVTLRRADIFELPFAP
                     ASFDHIFVCFVLEHLARPVQALQALKGVLKPGGSITAIEGDHGSAYFHPDSAFARRAV
                     QCLVELQGRAGGNALIGRTLYPLLSEAGFEGVRVSPRMVYVDSSKPELVEGFTRRTFT
                     AMVEGVRQLSISNGLASEASFDRGIADLNRTAEVDGVFCYTFFKAVGMKARDPFRLKR
                     RPS"
     gene            2556..3245
                     /locus_tag="VGA59_05840"
     CDS             2556..3245
                     /locus_tag="VGA59_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX9719234.1"
                     /translation="MSPRILAFDIFGTVVDWHGSIAREVDAMQLGVNGSDFALAWRAG
                     YKPAMQRVMSGELGWTLIDDLHRMILDGLLRQFGIDLDEARKKHLNKVWHRLDPWPDS
                     VEGLRRLKSRFMICTLSNGNIGLLADMAKRAGLPWDCILSAEVFRAYKPDAATYLGVA
                     RVFDVAPREVMLVAAHHDDLAGAHACGLGTAYIERPLEFGAGQPKDVSPRPENDLHAS
                     SLTALANMLGC"
     gene            3296..4228
                     /locus_tag="VGA59_05845"
     CDS             3296..4228
                     /locus_tag="VGA59_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9719235.1"
                     /translation="METKWLEDFVSLAETRSFSRSAQLRHVTQPAFSRRIQALEAWAG
                     TDLVDRSSYPTRLTPAGETLYAQSIEMLQALQSTRAMLRGHSSAGHDVIEFAVPHTLA
                     FTFFPAWVSSLREKFGPIKSRLIALNVHDAVMRLVEGGCDLLIAYHHASQPLQLDADR
                     YEMVALGQEVLSPYCKADASGEPMFKLPGRALQPLPYLGYAPGAYLGRVTDLILQQSS
                     TAIHLDRVYETDMAEGLKVMALEGHGVAFLPQSAVKKELRSRRLVSAAPPGVKDLEMT
                     MEVRAYREKPLGKEAPKGTAQALWAYLAAQTSNR"
     gene            4482..5378
                     /locus_tag="VGA59_05850"
     CDS             4482..5378
                     /locus_tag="VGA59_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9719236.1"
                     /translation="MKKHLLAFAVAALAAGSVFAQASDTIAKVKASGVITMGVRDSSG
                     ALSYTMGDGKYGGFHPDICQRIVANLEKAVGKKLEVRYLAVTSQNRIPLVQNGTVDIE
                     CGSTTNNATRAKDVSFVVTTYVEEVRIATKVKSGITSIAQLNGKNIATTTGTTSVQLL
                     RKHERAAGVDFKEIFGKDHADSFLLLESGRADAFVMDGQILAGNIATAKNPADYRIVG
                     EVLSVEPIAIMVRKGDTLKKLADDTVKDLIKTGELAKIYDKWFMQPIPPKNTRVGLPL
                     SETTKAAWANPNDKPAEEYAKK"
     gene            5547..6278
                     /locus_tag="VGA59_05855"
     CDS             5547..6278
                     /locus_tag="VGA59_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX9719237.1"
                     /translation="MSNWQWQVFLQDPGGQHPTYWEWLLSAWGWTVSVSLLSLLVALT
                     LGSIIGIIRTLPDSPLLARFGNAWVELFRNIPLLVQIFLWYHVFPALFLALRQVPDFL
                     LVVFALGFFTSARIAEQVRAGVQALPKGQRYAGMAMGFSTPQYYRYVLLPMAYRIIIP
                     PLTSESMNIFKNSSVAFAVSISELTMFAMQAQEETSRGIEIYMAVTALYIVSAFAINR
                     IMASIEKRTRVPGFIVSADAGAGGH"
     gene            6449..7138
                     /locus_tag="VGA59_05860"
     CDS             6449..7138
                     /locus_tag="VGA59_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX9719238.1"
                     /translation="MDIDFSFYNWELIRAFLLKGLYFSVVLTIVATVGGVIFGTVLAL
                     MRLSGIKLLVLPATVYVNGMRSIPLVMVILWFYLLVPAAFYSLFPYRLGENFRSEISA
                     VITFVAFEAAYFSEIMRAGIQSIPRGQVHAGQAVGMTYGQNMKLIVLPQAFRNMLPVL
                     LTQTIILFQDTSLVYAIGAYDLLKGFDAAGKNFGRPIESYLLAAAVYFVICYTLSWFV
                     KRLHKRIAIIR"
BASE COUNT         1318 a         2340 c         2292 g         1334 t
ORIGIN      
        1 tgatcgatgt cgaacgactt gaaccagaac attcaggcgc attacgccag gcctgacatc
       61 ggcaagcaga tccttgccgc gctggagaaa gcgggaaagg acatcgatcg gctcaccccg
      121 ggagacctcg cgccaatcga cgaattccac atccggggac gggccgcgac gctggagctc
      181 gcccgggccg caggcgtcga ttccacgaag aaggttcttg acgtcggcag tggcctcggt
      241 ggtccggcgc gcgcgctcgc gcgggagttc ggttgccagg tcaccggcat cgacctgacc
      301 gaagaatatt gccgcgtggc cgaaatgctg tctgggcgga tcgggctttc cactcatctc
      361 gaattcctgc aaggggatgc gctcgacctg ccttttgccg acgcgacatt cgacgtcgtg
      421 tggaccgagc acgtcgcgat gaacatcccg gacaagtccc ggctgtaccg ccagatgcac
      481 agggtactca agccgggagg cacgctggcg atctacgaca tcctcgccgg cccggccggc
      541 cccgtgcatt tccccgttcc ctgggctcgc gtgccgcaat cgagcttcct ggcaacgccg
      601 gacgagctgc gcctctttct cgatgaagcg ggtttcagga tcgccgactg ggcggatacg
      661 acggatctcg cccgggcatg gttcgccgca ctggccggga aagtccgcaa ggaaggcttg
      721 ccgccgctcg gattccatct tcttctgggc cccgagttca aggagatggc ccggaaccag
      781 cggcgcaatc tggatgaagg gcgcatcgtg ctcgcgcaga tcgtcgccag gaaatagcac
      841 gggctccggg gagcatccat gcccatgatg gatcaaggca tcgtcgtcct tggcatcccc
      901 atcctatcga gttcaaagcg aaaaaaaccg ctcatgggcg tatccctttg acccgcaatc
      961 cgtccaagcg tgtgggcgag ccgataagcg cgcccggaac tctttttttt ccacacttac
     1021 tctggatgga gatagccatg accaacctga cccccctcga gaccgaactg gtcgccttgg
     1081 gcgccgcact ggggagcaac tgcgttccct gcatcgagca ccacatccca caggcccgca
     1141 aagccggcct gagcgacctt cagatccgag ccgtcatcga actcgcggac aaggtgcggc
     1201 aagtccccgc ccgcaaagtg ctggagacgg ccgtccggct cctgggggag gagtccggcg
     1261 cccaggcagg cgggatggaa cgcgaatgtg ccagcatggg cggtgccgca agcaagtgct
     1321 gctgaccgct cctcgagccc gaggtctgcc gatgaagccg ggtggcggcg gatcacttcc
     1381 gctgatcgca agcagcaggc cgcggctcag ccgcgctgta ccgttgagct agagttgacg
     1441 ccgcaacccg cgtagctaaa ctttcggcgc ggcggccttg cgaagcgact cggaggcggg
     1501 tgtctggacc gtctcgatgt caccagcttt cgtcgccgcc atccaggtgg cctgaatcca
     1561 aggagtactg gccatgaagc cccggatcac gatgatcacc ctaggaaccg acgacctcga
     1621 agccgctggc tacgccggct attttcagga cccggatggc catctctggg aggtcgcgtg
     1681 gaatccccag atgccgccgc aagacgaagg acgactcata tgaccaaggc ttacgtgcac
     1741 ggatacgatc cacgggaagc cttgcggctg caggatcagg cgaccacgct ggtcgagctg
     1801 ttgcactcgg acacggccta tcccgcgggg cacaccgtgc tggaagccgg atgtggcgtg
     1861 ggcgcgcaaa cggtcacgct ggcgcgcaac agcccgaacg cgtcgatcac gtcgatcgat
     1921 ctgtccgaga cctcgctggc gaaggcgagg gcggcggtgc aagccgccgg agtgaccaac
     1981 gtcacgcttc ggcgggcgga cattttcgag ctgccgtttg cgcccgcatc cttcgaccac
     2041 atcttcgtgt gctttgtcct ggagcatctg gcccggccgg tgcaggcgct gcaggcgctg
     2101 aaaggcgtgc tgaagcccgg cggcagcatc acggcgatag aaggcgatca cgggtctgcc
     2161 tactttcacc ccgacagcgc gtttgcgcgt cgcgccgtcc agtgcctggt ggagttgcag
     2221 gggcgggcgg gcggcaatgc cttgatcggg cggaccctct atccgctgct cagcgaggca
     2281 ggcttcgagg gcgtgcgtgt ttctccacgc atggtctacg tggactccag caaacccgaa
     2341 ctggtggagg ggttcaccag gcggacattc accgccatgg tggagggggt tcgccaactc
     2401 tctatttcga atggtctcgc cagcgaggca agttttgatc gcggcatcgc cgatctgaac
     2461 cgtacggctg aggtcgatgg cgtgttctgc tatacgtttt tcaaggcggt cgggatgaaa
     2521 gcccgagacc catttcgtct gaagcgcagg ccatcgtgag cccccgcatc ctcgccttcg
     2581 acatcttcgg cacggtggtc gactggcacg gcagtattgc ccgtgaagtg gatgccatgc
     2641 agcttggcgt gaacggcagc gacttcgcgc tggcctggcg ggccggctac aaacctgcga
     2701 tgcagcgcgt catgtccggc gagctcggct ggaccctgat cgacgacctg caccgcatga
     2761 tcctcgacgg attgctgcgg cagttcggca tcgacctcga tgaagcgcgg aagaagcacc
     2821 tgaacaaggt gtggcaccgg ctcgatccct ggccggattc ggtggaaggg ctgcggcggc
     2881 tgaagtcacg cttcatgatc tgcacgctgt cgaacggcaa catcggtttg ctggccgaca
     2941 tggccaagcg cgccggcttg ccgtgggact gcattctctc ggccgaagtg ttccgtgcct
     3001 acaagcctga tgcggcgaca tacctgggcg tggcgcgcgt gttcgatgtg gctccgcggg
     3061 aggtcatgct ggtggcggcg caccacgacg acctggccgg tgcgcatgcc tgcggcctgg
     3121 gcacggccta tatcgagcgg cccctggagt tcggtgccgg ccagccgaaa gacgtgtcgc
     3181 cccggccgga aaacgacctg cacgcgtcca gcctcaccgc gctggcgaat atgctgggtt
     3241 gctgacggcg ggccccaacc tggttccagc ctggctgcgc gcaataatcg cggccatgga
     3301 aaccaaatgg ctggaagact tcgtcagtct ggccgaaacc cgcagtttca gccgctcggc
     3361 gcagttgcgc cacgtcactc agcccgcctt ctcgcgccgc atccaggcgc tggaggcctg
     3421 ggccggcacc gacctggtgg accgcagctc gtatcccacg cgcctcacgc cggccggcga
     3481 aacactctat gcgcagtcga tcgagatgct gcaggcgctg cagagcacgc gcgcgatgct
     3541 gcgcggccac tcttcggcgg gtcacgacgt gatcgaattc gccgtgcccc acacgcttgc
     3601 gttcacgttc ttcccggcct gggtgtcttc cctgcgggag aagttcggac cgatcaagag
     3661 ccggctgatc gcgctcaacg tgcacgacgc cgtcatgcgc ctggtggaag gcggctgtga
     3721 cctgctgatc gcctatcacc atgcctcgca gccgctccag ctcgatgccg atcgctacga
     3781 gatggtggcc ctcggccagg aggtgctgtc cccctactgc aaggcggacg cgagtggcga
     3841 gccgatgttc aagctgccgg gccgggctct ccagcctttg ccttacctcg gttacgcacc
     3901 cggcgcctac ctggggcgcg tcacggacct gatactgcag caatcgagca ccgccatcca
     3961 cctggaccgc gtctatgaaa ccgacatggc cgagggcctg aaggtgatgg cgctcgaagg
     4021 ccacggcgtc gctttcctgc cgcagagcgc ggtgaagaaa gagctgagat cgcggcggct
     4081 ggtcagtgcg gccccgcccg gggtgaagga tctggagatg acgatggaag tgcgcgctta
     4141 tcgggagaaa cccttaggca aggaggcgcc gaaagggacg gcgcaggcgc tgtgggccta
     4201 cctggcggcc caaaccagca acagatgagg agtttcatgg gttataagtt tcttgcataa
     4261 cagcgcgcgt aaacagcatt ggcgttccac aaggggcgcc gttacagttc gcgccagccg
     4321 caaaaccaag ccctagcgat gtcatccaac ttccgagtcg aacacgattt cctgggcgag
     4381 aagcagatcc cggccgattc gtattggggc gtgcacacgg cccgcgcgaa tccctagaat
     4441 ttcgttctca gtaacgtaac gcttcaccac aggagaaaag catgaagaag catttgttgg
     4501 cattcgcagt cgcggccctg gccgccggca gcgtcttcgc gcaggcgagc gacaccatcg
     4561 ccaaggtcaa ggcctcgggc gtcatcacca tgggggtgcg cgattcctcg ggtgctctct
     4621 cgtacacgat gggtgatggc aaatacggtg gcttccatcc tgatatctgt cagcgcatcg
     4681 tcgccaacct ggaaaaggcc gtcggcaaga agctggaagt caggtacctt gcggtgacct
     4741 cgcagaaccg cataccgctg gtgcagaacg gcaccgtgga catcgagtgc ggctccacca
     4801 ccaacaacgc cacgcgggcg aaggacgtct ccttcgtcgt taccacgtac gtcgaggaag
     4861 tgcgcattgc caccaaggtc aagtcgggca tcacctcgat cgcgcagctc aatggcaaga
     4921 acatcgcgac caccaccggc accacctcgg tgcagctgct gcgcaagcat gagcgcgctg
     4981 ccggcgtcga cttcaaggag atattcggca aggaccacgc cgacagcttc ctgctgctcg
     5041 aatcgggccg cgccgacgcc ttcgtgatgg atggccagat cctggccggc aacatcgcca
     5101 cagccaagaa ccccgccgac taccgcatcg tgggcgaggt gctgagcgtg gagccgatcg
     5161 ccatcatggt gcgcaagggc gacacgctca agaagctggc cgatgacact gtcaaggacc
     5221 tgatcaagac cggcgagctg gccaagatct acgacaagtg gttcatgcag ccgattccgc
     5281 cgaagaacac gcgcgtggga ctgcccctct cggagaccac gaaggcggcc tgggccaacc
     5341 cgaacgacaa gccggccgaa gagtacgcca agaagtaatc gcgtggcgct tgcaatgaaa
     5401 gcccgctcgc tcgtgcgcgg cgggcttttt ctcgccggga cctggatggc aggctttaaa
     5461 gcttgaaata cccgctgggt tccagcaaca tggcatgcgg cctgctttgg tgcacatacc
     5521 gataagagtc tcgaggagca tcttcaatgt cgaactggca atggcaagtc tttctgcagg
     5581 acccgggcgg acagcacccg acctattggg aatggctgct ctcggcgtgg ggctggaccg
     5641 tttccgtctc cctgctctcg ctgctggtgg cgctgacgct cggctccatc atcggcatca
     5701 tccgcacgct gcctgacagt ccgttgctgg cgcggttcgg caatgcctgg gtggagctgt
     5761 tccggaacat ccccttgctg gtgcagattt tcctgtggta ccacgtcttc ccggcgctgt
     5821 tcctggcatt gcgccaggtg cccgacttcc tgctggtggt gttcgcgctg ggcttcttca
     5881 cttcggcgcg catcgccgaa caggttcgcg ccggcgtgca ggccctgccc aagggacagc
     5941 gctacgccgg catggcgatg ggcttcagta caccgcagta ctaccgctac gtgctgctgc
     6001 cgatggctta ccgcatcatc attccgccgc tcaccagcga gtcgatgaac atcttcaaga
     6061 attcctcggt ggccttcgcc gtgtcgatct cggagctgac catgttcgcc atgcaggcgc
     6121 aggaggaaac ctcgcgcggc atcgagatct acatggcggt cacggccctg tacatcgttt
     6181 ccgcgttcgc catcaaccgc atcatggcga gcatcgaaaa aagaacacgg gtgcccggtt
     6241 tcatcgtgtc cgccgacgcg ggtgccggag ggcactgaag tgaaagccca cccccgaagc
     6301 gccttcggcg atgccccctc gggcttcgcc ctctccccct cgagggggcg ccaccagcgg
     6361 cccggcaaag ccggttccgc ggtggccccc gcactggccg cacgatttca tacgttgccg
     6421 gtggtgcgca gcgccatgga gaactgacat ggatatcgat ttctccttct acaactggga
     6481 actcatccgc gccttcttgc tgaagggctt gtatttcagc gtcgtgctga cgatcgtcgc
     6541 cacggtggga ggcgtgatct tcggcacggt gctcgcgctg atgcggctgt cgggaatcaa
     6601 actcctggtg ctgcccgcca cggtctacgt caacggcatg cgcagcatcc cgctggtgat
     6661 ggtgatcctg tggttctacc tgctggtgcc ggcggcgttc tattcgctgt tcccgtaccg
     6721 gctgggcgag aacttccgct ccgagatctc cgcggtgatc accttcgtcg cgttcgaggc
     6781 ggcctacttc agcgagatca tgcgcgcggg catccagtcg atcccgcgcg gccaggtcca
     6841 tgccgggcag gccgtgggca tgacctatgg ccagaacatg aagctcatcg tgctgcccca
     6901 ggccttccgc aacatgttgc cggtgctgct gacgcagacc atcatcctgt tccaggatac
     6961 ctcgctggtg tacgcgatcg gcgcctatga cctgctcaag gggttcgatg ccgccggcaa
     7021 gaatttcggc cggccgatcg agtcgtacct gctggcggct gccgtctact ttgtcatctg
     7081 ctacaccttg tcatggttcg tcaagcgcct gcacaagagg attgcaatca ttcgctaatg
     7141 aagcccaccc ccgaagcgcc ttcggcgatg ccccctcggg cttcgccctc tccccctcaa
     7201 gggggccgag acctgcggct ccaaaccgac ctgcgtcggt ttggacaggt cgaggaatgg
     7261 ccgcgtcttg cggccatgcg gcct
//
LOCUS       DASWIY010000156        13620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3403018, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000156 DASWIY010000000
VERSION     DASWIY010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 13620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13620
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3403018"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /gene="hslU"
                     /locus_tag="VGA59_05865"
     CDS             <1..173
                     /gene="hslU"
                     /locus_tag="VGA59_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806774.1"
                     /note="heat shock protein involved in degradation of
                     misfolded proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HEX9719239.1"
                     /translation="ENIGARRLATVMERLLDEVSFDATRLGGQTVTIDAGYVDARLKE
                     LSRDDDLSRYIL"
     gene            418..846
                     /gene="mraZ"
                     /locus_tag="VGA59_05870"
     CDS             418..846
                     /gene="mraZ"
                     /locus_tag="VGA59_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX9719240.1"
                     /translation="MFQGASSLNLDAKGRLSVPTRHRDVLGATAANQLTITRHPHGCL
                     MIFPRPEWEKFRDRIAALPMEAQWWKRIFMGNAMDVELDGTGRVLISPELRTAAGIEK
                     ETMLLGMGNHFELWDKAKYEAQEAEAMRQPMPDVFKEFSF"
     gene            856..1770
                     /gene="rsmH"
                     /locus_tag="VGA59_05875"
     CDS             856..1770
                     /gene="rsmH"
                     /locus_tag="VGA59_05875"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX9719241.1"
                     /translation="MDSTWQHTTVLLNEAVAALITKPDATYVDATFGRGGHARLILSK
                     LSTQGRLIAFDKDPDAIAEAGRVDDSRFSIRHEGFRHLGELPAASVAGVLLDLGVSSP
                     QIDNPARGFSFRSDGPLDMRMDTTRGESVADWLATAQAGQIAEVIRDYGEERFAVQIA
                     KAIVARRQERGPLATTGELAQLVAGAVKTREPGQDPATRTFQALRIFINSELEELQQA
                     LEASLDILQPQGRLVVISFHSLEDRIVKQFIVRHSREVFDRRAPFAPPRAMKLKALDR
                     VRASAAEVAANPRARSAIMRVAERTGDR"
     gene            1767..2075
                     /gene="ftsL"
                     /locus_tag="VGA59_05880"
     CDS             1767..2075
                     /gene="ftsL"
                     /locus_tag="VGA59_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX9719242.1"
                     /translation="MTRLNLVLLLAVLASALYLVRTQYESRRLFAEIDRAVSEARKLE
                     IEHERLQVEKRAQATPLRVEKLAKEQLQMRNASPAITQYVSLKGATAAAAPAASGVVP
                     "
     gene            2072..3805
                     /locus_tag="VGA59_05885"
     CDS             2072..3805
                     /locus_tag="VGA59_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX9719243.1"
                     /translation="MSRSLAYTSSPLLASKTPVWRSKFIVAAIATGFVTLAARAAWVQ
                     VFSNDFFQRQGQVRFARTLELPANRGRILDRNGLLLATSVPAPSFWAIPEDVERDRAK
                     LARLARLLEIPMADLDRKLQDEDKTFVWLKRQVDEAVAQQVAALDIKGIYQRKEYKRQ
                     YPEGEAAAHVVGFTNVEDRGQEGVELAFNDQLAGRPGSRRVIKDRLGRVVEDVGEQVP
                     PVDGRDLQLSIDSKVQFFAYQKLRDAVLEHKAKAGSVVVLDVVSGEVLALANYPSYGP
                     NRRANLTGAQLRNRALTDTFEPGSTMKPFVIALALEKGLVRPDTPIQTAPGRLMIGGS
                     MISDAHPHGVLTVSEVIQKSSNVGTVKMAMQMQPREMWEMFSQVGFGQKPQLPFPGAV
                     SGRLRAYKGWRPIEQATMSYGYGLSGSLFQLARAYSVFARDGEMVPVTLLRNPDSAAG
                     LRVMEAATAQAVRGMLHRVTLSGGTAPKAQTMGYSVGGKTGTARKQEGKGYADKKYRA
                     FFVGMAPVDKPRIVVAVMIDEPGAGKYFGGDVAAPVFAATVQQTLRMLGVQPDMNVKP
                     QIVVQAVEESF"
     gene            3805..5280
                     /locus_tag="VGA59_05890"
     CDS             3805..5280
                     /locus_tag="VGA59_05890"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX9719244.1"
                     /translation="MLELHNFREAAGWLHSRVTGTLHCDSRAIASGDGFIAWPGAATD
                     GRMYVHSALAQGAAACLVEREGVQAFDFDTPAVATYAQLKAATGPIAAAYFGAPTERL
                     EVLAVTGTNGKTSTAWWLAQALSNLQPAISCGLIGTLGIGRPPHVGFVGLTTPDPVLL
                     QRQFRRFLDDGLKACAIEASSVGIVERRLDGTHVRVAVFTNFSQDHLDYHGTMEAYWE
                     AKAELFRWPGLRSAVVNLDDEKGLALFHSLEGNVELWTTSCEGPARLRAQDIDYSDHG
                     LRFTVQEGEQRHSLATRLIGQYNVSNLLGVIGAMRALGVDLAPAVQACGSLSPVPGRM
                     ECLREPGKPVVAIDYAHTPDALDKALQALGPLARQRGGKLWCVFGCGGDRDAIKRPLM
                     AAVVEKNADHVVVTSDNPRREKPENIISQILLGLSHDECVQVQADRALAIAETVAAAA
                     AEDVVLLAGKGHEDYQEVAGVKHPFSDKVHAQAALDARGAA"
     gene            5489..6865
                     /gene="murF"
                     /locus_tag="VGA59_05895"
     CDS             5489..6865
                     /gene="murF"
                     /locus_tag="VGA59_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX9719245.1"
                     /translation="MFTLAEALASLPGGALVGDGQVAVERVHSDTRTLQPGDLFVALK
                     GERFDANDFLAEAQAKGAAAAIAHRGKLPPGFPGIEVDDSKLALGSLAAGWRARFGLP
                     LIAVTGSNGKTTVTQMIASILRAWLPESMLATQGNLNNDIGLPLQLLRLRAGHRVAVL
                     ELGMNHPDEIAYLAGICRPTVALVNNAQREHQEFMATVEAVARENGSVFAALGPSGVA
                     VFPADEEFTPVWLELARARACMTFGDANADIVLAGSEWQDGHWNVSVKTPAGPIAFAL
                     HIAGRHNVRNSLAAAACALAAGVPLAAIAQGLQSFEPVKGRSRALAVRLGGRTITVVD
                     DTYNANPDSVRAAVDVLAELPGPRLLVLGDMGEVGNQGPQFHAEVGEHARERGIEQLM
                     TLGEQSVAMKGRHFDAIEPLNAAVLAELPRVASVLVKGSRFMKMERVVEAITAQAEQQ
                     QPEPGHAA"
     gene            6855..8033
                     /gene="mraY"
                     /locus_tag="VGA59_05900"
     CDS             6855..8033
                     /gene="mraY"
                     /locus_tag="VGA59_05900"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX9719246.1"
                     /translation="MLLSLAQWLQTLSPEFGFLRVFQYITFRAVMAAMTSLLIGLIFG
                     PAVIRKLASLKIGQPVRGYGLQSHLSKTGTPTMGGVLVLLSIAVSTLLWFDLTNRFVW
                     IVLAVTLGFGAIGWVDDWRKVVRKDPEGMRSREKYFWQSLIGLLAALYLVFSISESSN
                     WRVLQLFFTWVRSGFDVDLPPKAGLLLPFFKEVSYPLGVFGFVILTYLVIVGSSNAVN
                     LTDGLDGLAIMPVVMVGSALGVFAYVTGNVVFSRYLFFPHIPGTAELLIFCAAMAGAG
                     LAFLWFNTHPAQVFMGDVGALALGAALGTIAVIVRQEIVLAIMGGVFVVEAISVMAQV
                     MYFKYTKKRFGEGRRILKMAPLHHHFEKSGWTETQVVVRFWIITMLLCLVGLSTLKLR
                     "
     gene            8036..9589
                     /gene="murD"
                     /locus_tag="VGA59_05905"
     CDS             8036..9589
                     /gene="murD"
                     /locus_tag="VGA59_05905"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX9719247.1"
                     /translation="MHELKDRHVLILGLGASGLAMARWCARCGAMVTVADTREDPPQL
                     QALREQLPQARFTGGPFGADLVSEEVQAVFRSPGLSPEQVAVVACAAGERGTPVGGEL
                     SLFADALAGLRAREGYAPTVLAVTGTNGKTTVTALAGQLVERSGRTVAVAGNIGPTLL
                     DTLAGKIDSGALPQVWVIELSSFQLDTPGEFESTAAAVLNVTQDHLDWHGSMEAYAGA
                     KARIFGKNALMILNREDPLVMGMVPEPIRVKGGRMEQRPYLTFGGDAPQRPGDFGIEV
                     VNGMAWLVRAQEADETQRRRPRSLQARELAAAQAGADEELYIQRLMPADALRIRGRHN
                     AVNALSALALAGAAGCPLGPMLYGLREYRGEPHRVEPVGIINQVEYFDDSKGTNVGAT
                     VAALQGLGAERKVIVILGGDGKGQDFSPLAAPVSRHARAAVLIGRDAPLIRAVLEPAG
                     VPLIDAGSMREAVSLATERASPGDAVLMSPACASFDMFRNYPHRAEVFRAAVQGIADE
                     AGVQLEGAA"
     gene            9789..11033
                     /gene="ftsW"
                     /locus_tag="VGA59_05910"
     CDS             9789..11033
                     /gene="ftsW"
                     /locus_tag="VGA59_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX9719248.1"
                     /translation="MSLQAVIAGLIPNPLASTAKPIRTRRDRHLGKPASVRVQGVDQP
                     LLWVTVALLAFGLVMVYSATVALPDNPKFARYSHTYFLVRHIVFLALGLVAALMAFQV
                     PIATWEKIAPWLFVASLVLLMLVLIPGVGSVVNGARRWISVGFMGFQPSELAKFAVLL
                     YAADYMVRKMDVKERFFRAVLPMAAAVAVVGLLLLAEPDMGAFMVIAVIAMGILFLGG
                     VNARMFFLIASVITIAFVLMIALSDWRRERIFAYLDPWSEKYALGKGYQLSHSLIAIG
                     RGEIFGVGLGGSVEKLHWLPEAHTDFLLAVIGEEFGLIGVVTVIVAFLWMTRRIMHIG
                     RQAIALDRVFAGLVAQGVGIWIGFQAFINMGVNLGALPTKGLTLPLMSYGGSAILMNL
                     VAIAVVLRVDYENKVLMRGGRA"
     gene            11242..12312
                     /gene="murG"
                     /locus_tag="VGA59_05915"
     CDS             11242..12312
                     /gene="murG"
                     /locus_tag="VGA59_05915"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX9719249.1"
                     /translation="MTSAKCALVMAGGTGGHIFPGLAVAEALRERAWRVHWLGGSASM
                     ESQLVPPRGFAFETIDFSGVRGKGVKTLVLLPLRLLRAFWQSIAVVRRVKPDVVIGLG
                     GYITFPAGMMSVLLGKPLVLHEQNSVAGMANRILAGVADRVFTAFPHTLGKAAWVGNP
                     LRAAFLQQPGPAERMAAREGPLRLLVVGGSLGARALNDIVPKALALIPANERPVVLHQ
                     SGAKQIDELRANYAAAGVQAELTPFIEDTARAFAEADLIVCRAGASTVTEIAAVGAAA
                     LFVPFPSAVDDHQTTNAKFLVDAGGGWLVQQRDLRPEWLAHLVRTTGRPELIRRALAA
                     RSMSKTSATAEVVKACEELAKP"
     gene            12309..>13620
                     /gene="murC"
                     /locus_tag="VGA59_05920"
     CDS             12309..>13620
                     /gene="murC"
                     /locus_tag="VGA59_05920"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX9719250.1"
                     /translation="MKHAVKHIHFVGVGGAGMSGIAEVLRNLGYLISGSDLADSPTLR
                     RLEALGIKTYLGHAASHVIGADAVVTSTAVQADNPEVLAARQRKIPVVPRAMMLAELM
                     RLKQGIAIAGTHGKTTTTSLVASVLAEGGLDPTFVIGGRLNSAGANAKLGGGDYIVVE
                     ADESDASFLNLMPVMAVVTNIDADHMETYGHDFNKLKGAFLDFLHRMPFYGTAILCID
                     DPAVREIVPDVQCPITSYGLGEEAQVRAIDVRAVGTQMHFTVQRRNGVSLPDLKVVLN
                     LAGMHNVLNALSAIAVAVELNIPDQAVLKALAEFTGVGRRFQNYGQVAVLPENGGGHF
                     TVIEDYGHHPVEIVATLSAARGAFPGRRLVLAFQPHRYTRTRDCFEDFVKVLGHADAV
                     LLAEVYSAGEAPIVAADGRSLARAVRVAGKVEPIFVDPIDAMPLA"
BASE COUNT         2214 a         4428 c         4744 g         2234 t
ORIGIN      
        1 ccgaaaacat cggggcccgg cggctggcca ccgtcatgga gaggctgctg gacgaggtca
       61 gcttcgatgc cacgcgcctg ggcgggcaga ccgtgaccat cgatgcgggc tatgtcgacg
      121 cacgcctgaa ggaattgagc cgcgacgacg atttgtcgcg ctacattctc taacactcgc
      181 gccgctgctg aggattcact ttcacagcaa acgctaagtc gttcattcag aacaggtttt
      241 gccgcttatt gatgtgcaga atcggcgcta agtcgttgat ttcattgcga taaccgggcg
      301 attacccgcc ttgcgccgca gagttttcct gctacagtgc aaaaaagtgc aattaagtgg
      361 tgaatagtgc cttcgcactc cactattgcg gctccactgt agcaaggact cacacaggtg
      421 tttcaaggcg cttcgtcact gaatctcgat gccaaaggcc ggctttccgt gccgacccgg
      481 catcgcgacg tgctgggcgc caccgcggcc aaccagctga cgatcacccg ccacccgcac
      541 ggctgcctga tgatattccc ccgaccggaa tgggaaaaat tccgcgaccg tatcgccgcg
      601 ttacccatgg aggcccagtg gtggaagaga atcttcatgg gcaacgcgat ggacgtggag
      661 ctggacggca ccggccgggt gctgatctct ccggaattgc gtaccgcggc gggcatcgag
      721 aaggaaacca tgctgctggg catgggcaac cacttcgagc tctgggacaa ggcgaagtac
      781 gaggcgcagg aagcggaggc gatgcggcaa ccgatgcccg acgttttcaa ggaattttcc
      841 ttttgagagc aggcggtgga cagcacatgg caacacacca ccgtcttgtt gaacgaagcg
      901 gtcgcagctc tcatcaccaa gccggacgcg acttatgtcg acgcgacctt cgggcgcggc
      961 ggccacgcgc gtctgatcct ctcgaagctc tcgacgcagg ggcgcctgat cgcattcgac
     1021 aaggacccgg acgcgatcgc cgaggccggc cgcgtggacg attcgcgctt ctcgatccgg
     1081 cacgagggct ttcgccacct cggcgagctg cccgctgcga gcgtggccgg cgtgctgctg
     1141 gacctgggcg tgagctcgcc gcagatcgac aacccggcgc gcggcttttc gttccggtcc
     1201 gacggaccgc tggacatgcg catggacacc acgcgtggcg agagcgtggc cgattggctc
     1261 gccacggcgc aggccgggca gatcgcggag gtgattcgtg actacggcga agaacggttt
     1321 gctgttcaga ttgcaaaggc gattgtggct cgccgacagg aacggggccc tcttgcaacc
     1381 accggcgaac tggcccagct cgtggctggc gcggtcaaaa cccgcgagcc gggccaggac
     1441 cctgcaacgc gcacatttca ggctcttcgg attttcatca attccgagct tgaagagctg
     1501 caacaggcgc tagaggccag cttggacatc ctgcaacccc agggccggct cgtggtgatc
     1561 agcttccatt cgctggagga ccgcatcgtc aagcaattca tcgtgcggca ctcgcgcgag
     1621 gtattcgatc gccgcgcgcc gttcgcgccg cccagggcga tgaagctcaa ggcgctggac
     1681 cgggtcaggg cgtcggcggc cgaagtggcg gccaacccgc gcgcgcgcag cgcgatcatg
     1741 cgcgtggcgg aaaggacggg ggaccgatga cacgtctgaa cctcgtcctg ttgctggccg
     1801 tgctcgcgag cgcgctgtac ctggtgcgca cgcaatacga gtcgcggcgg ctgttcgccg
     1861 agatcgacag ggccgtaagc gaggcgcgca aactcgagat cgagcacgag cgcctgcagg
     1921 tggaaaagcg cgcgcaggcc acgccgctgc gcgtggaaaa gctggcgaaa gagcagttgc
     1981 agatgcgaaa cgcgtcgccc gccatcacgc agtatgtcag cctgaaaggc gcgaccgcgg
     2041 ccgcggcccc ggccgcgagc ggagtggttc catgagtcgc agtctcgcct acacctcgag
     2101 cccgctgctc gcgagcaaga cgccggtgtg gcgcagcaag ttcatcgtcg cggccatcgc
     2161 gacgggcttc gtcacgctgg cggcccgcgc ggcctgggtc caggtgttct ccaacgattt
     2221 tttccagaga cagggccagg tgcgctttgc ccgaaccctg gaactgcccg ccaatcgcgg
     2281 ccgcatcctg gaccgcaatg gcctgctgct ggccaccagc gtgccggcgc ccagtttctg
     2341 ggcgatcccc gaagacgtgg aacgcgaccg cgccaagctg gcccggctcg ccaggctgct
     2401 ggaaataccg atggccgatc tcgaccggaa gctccaggac gaggacaaga ccttcgtctg
     2461 gctcaagcgc caggtggacg aagccgtggc ccagcaggtc gccgcgctgg acatcaaagg
     2521 catctaccag cgcaaggaat acaagcgcca gtacccggaa ggcgaagccg ccgcgcacgt
     2581 ggtgggattc accaatgtgg aagaccgggg ccaggaaggc gtggaactcg cgttcaacga
     2641 ccagctggcg gggcggccgg gctcgcgccg cgtgatcaag gaccggctgg gccgggtggt
     2701 cgaggacgtg ggcgagcagg tgccgccggt ggacgggcgc gacctgcagc tgtcgatcga
     2761 cagcaaggtg cagttcttcg cctatcagaa gctgcgcgac gccgtgctgg agcacaaggc
     2821 caaggccggc agcgtggtcg tcctggacgt ggtcagcggc gaagtgctgg cactggccaa
     2881 ttaccccagc tacggaccga accggcgcgc caatctgacg ggcgcgcagt tgcgcaaccg
     2941 ggccctgacc gacaccttcg agccgggctc gacgatgaag ccttttgtca tcgcgctcgc
     3001 actcgaaaag ggcctggtgc gacccgacac gccgatccag accgcgccgg gacggctgat
     3061 gatcggcggc tcgatgatca gcgacgcgca tccgcacggc gtgctcaccg tgagcgaggt
     3121 gatccagaag tccagcaacg tcggcaccgt gaagatggcg atgcagatgc agccgcgcga
     3181 gatgtgggaa atgttctcgc aggtgggctt cggacaaaaa ccgcagttgc ccttccccgg
     3241 cgccgtgagc gggcggctgc gcgcctacaa aggctggcgg ccgatcgagc aggccaccat
     3301 gagttacggc tacggcctgt cgggcagcct gttccagttg gcgcgcgcat acagcgtgtt
     3361 cgcgcgcgac ggcgagatgg tgccggtgac gctgctcagg aatcccgata gcgccgccgg
     3421 cttgcgggtc atggaggcag ccacggcgca ggccgttcgg ggcatgcttc accgggtgac
     3481 gctttccggc ggcacggcgc cgaaggccca gaccatgggc tactcggtgg gcggcaagac
     3541 cggcaccgcg cgcaagcagg agggcaaggg ctacgcggac aagaaatacc gggccttctt
     3601 cgtgggcatg gcgccggtgg acaagccgcg catcgtggtc gcggtgatga tcgacgagcc
     3661 cggcgccggc aagtacttcg gtggcgacgt agccgcgccg gtgttcgccg ccacggtgca
     3721 gcagacgctt cgcatgctgg gcgtccagcc cgacatgaac gtcaagccgc agatcgtggt
     3781 gcaggcggtg gaggagtcgt tctgatgctc gaactccaca atttccgcga agccgccggc
     3841 tggctgcaca gccgcgtcac ggggacgctg cactgcgaca gccgggcaat cgcatcgggc
     3901 gacggcttca tcgcctggcc gggcgcggcg accgacgggc gcatgtatgt gcactcggcg
     3961 ctggcgcagg gcgcggcggc ctgcctggtg gagcgcgagg gcgtgcaggc gttcgacttc
     4021 gacacgcccg ccgttgccac ctacgcgcag ctcaaggcgg ccaccggacc catcgcggcg
     4081 gcctatttcg gcgcgcccac cgagcggctc gaagtcctgg ccgtcaccgg caccaacggc
     4141 aagacttcca cggcatggtg gctggcacag gccctgtcca acctgcagcc ggccatttcc
     4201 tgcggcctga tcgggacgct gggaatcggc cggccgcctc atgtgggttt cgtcggtctg
     4261 accacccccg acccggtgct gctgcaacgc cagttccgcc gcttcctgga cgacgggttg
     4321 aaagcctgcg ccatcgaggc ctcttccgtg ggcatcgtcg agaggcggct tgacggcacg
     4381 catgtccgcg tagccgtgtt caccaacttc agccaggatc acctggacta ccacggaacg
     4441 atggaggcct actgggaggc caaggccgaa ctgttccgct ggcccggctt gcgctcggcc
     4501 gtcgtcaatt tagacgacga gaaaggactg gcgcttttcc actccctcga gggcaacgtc
     4561 gaactgtgga ccacgtcgtg cgaagggccg gcccggctga gggcgcaaga catcgattac
     4621 agcgatcacg gcctgcgctt cacggtgcag gagggcgagc agaggcattc gctggcgacc
     4681 cggctgatcg gccagtacaa cgtgtccaat ctgctcgggg tgatcggcgc catgcgggcg
     4741 ctgggagtcg atctcgcgcc cgccgtgcag gcgtgcggca gcctgtcgcc cgtgccggga
     4801 cggatggagt gcctgcgcga gcccggcaag cccgtggtcg ccatcgacta tgcgcacacg
     4861 cccgatgcgc tggacaaggc gctgcaggcg ctggggccgc tggcgcggca gcgcggcggc
     4921 aagctgtggt gcgtgttcgg ctgcggcggc gatcgcgacg cgatcaagcg accgctgatg
     4981 gcggctgtgg tggagaagaa cgccgaccat gtggtggtca caagcgacaa cccgcgccgc
     5041 gagaaaccgg agaacatcat cagccagatc ctgctgggcc tgtcgcacga cgaatgcgtc
     5101 caggtgcagg ccgaccgcgc actggccatt gccgagacgg tcgcggccgc ggccgccgag
     5161 gacgtggtgc tgctggccgg caagggccac gaggattacc aggaagtggc cggtgtcaag
     5221 catccgttct ccgacaaggt ccatgcgcag gcggcgctgg atgcgagggg cgcggcatga
     5281 gcgcgaaggg cgaggcagcg ccggaacgtg gcgcctcggt acgtgcgcag tggaggcgct
     5341 gcgacgattc ggctgcgcgg agcagctgcg caggctctgc cgctgcgccg aatccacgcg
     5401 tgcggggatc gaccagcgcg aatggcgcaa gcggccgacc cccgggggcg tatctgaact
     5461 tcgcgctttc ggaggccgcg gcatgagcat gttcacgctg gccgaggccc tggcatccct
     5521 tcccgggggc gctctcgtgg gcgacggcca ggtggcggtg gagcgcgtcc atagcgacac
     5581 acgcacgctg cagcccggcg acctgttcgt cgcgctcaag ggcgagcgtt tcgacgccaa
     5641 cgatttcctg gccgaagcgc aggccaaggg cgcggccgcc gcgatcgccc atcgcggcaa
     5701 actgccgccc ggctttcccg gcatagaggt cgacgacagc aagctggcac tcggctcgct
     5761 ggccgccggc tggcgcgcgc ggttcggcct gcccctgatc gcggtgaccg gcagcaacgg
     5821 caagactacg gtcacgcaga tgatcgcctc catcctgcgc gcctggctgc ctgaatcgat
     5881 gctggccacg caaggcaacc tcaacaacga catcgggttg ccgctgcaac tgctgcggct
     5941 gcgcgccggg caccgcgtcg ccgtcctgga actgggcatg aaccatccgg acgagatcgc
     6001 gtacctggcc ggcatctgca ggcccaccgt ggccctggtc aacaacgccc agcgcgagca
     6061 ccaggaattc atggccaccg tggaagcggt ggcgcgggag aacgggtcgg tgttcgccgc
     6121 cctcggaccc agcggcgtgg ccgtgtttcc cgccgacgag gagttcacgc ctgtgtggct
     6181 ggaactcgcc cgcgcgcgcg cctgcatgac tttcggcgac gccaacgccg acatcgttct
     6241 ggccgggtct gaatggcaag acgggcattg gaacgtgagc gtcaaaacgc ccgccggtcc
     6301 gatcgccttt gctctccata tcgccggccg ccataacgtg cgcaactcgc tggctgccgc
     6361 ggcctgcgca ttggcagcgg gcgtgccgct ggcggcgatc gcccagggcc ttcagtcctt
     6421 cgagccggtc aagggccggt cccgggccct ggcggtcagg ctgggcggac gcacgatcac
     6481 cgtggtggac gacacctaca acgccaatcc ggattcggtg cgcgccgccg tcgacgtgct
     6541 ggccgaactg cccgggccgc gcctgctggt gctgggcgac atgggtgagg tgggcaacca
     6601 gggtccgcag ttccatgccg aggtgggcga gcacgcgcgc gagcgcggca tcgagcagtt
     6661 gatgacgctg ggcgagcagt cggtggccat gaagggccgc cacttcgacg ccatcgagcc
     6721 gctcaacgcc gccgtgctgg ccgagttgcc ccgggtcgcc agcgtgctgg tcaagggctc
     6781 gcgcttcatg aagatggagc gcgtggtcga ggcgatcacg gcccaggccg aacaacaaca
     6841 accggaaccc ggccatgctg cttagccttg cccaatggct gcaaacgctg tcgcccgagt
     6901 tcggcttcct gcgcgtgttc cagtacatca cgttccgtgc cgtgatggcg gccatgacct
     6961 cgctgctgat cgggctgatc ttcggtcccg cggtcatccg caagctcgcc tcgctgaaga
     7021 tcggccagcc ggtgcgcggc tatggcctgc aatcgcatct gagcaagacg ggcacgccca
     7081 ccatgggagg cgtgctggtg ttgctgtcca tcgccgtttc cacgctgttg tggttcgacc
     7141 tcaccaaccg gttcgtctgg atcgtgctgg ccgtcacgct cggtttcggc gcgatcggct
     7201 gggtcgacga ctggcgcaag gtggtgcgca aggatcccga aggaatgcgc tcgcgcgaga
     7261 agtatttctg gcaatcgctg atcgggctgc tggccgccct gtacctggtg ttcagcattt
     7321 cggaaagttc caactggcgg gtgctccaac tcttcttcac ctgggtgaga tcgggcttcg
     7381 acgtcgatct gccgcccaag gcgggtctgc tgctgccgtt cttcaaggaa gtgagctacc
     7441 cgctgggtgt gttcggattc gtgatcctca cttacctggt gatcgtgggc tccagcaatg
     7501 cggtgaatct caccgacgga ctggacgggc tggcgatcat gccggtggtg atggtcggct
     7561 ccgcgctggg ggtcttcgcg tacgtgacgg gcaatgtcgt gttttccagg tacctgtttt
     7621 ttccccatat cccgggtacc gccgaactgc tgatcttctg cgcggcaatg gccggtgcgg
     7681 ggctggcttt tctctggttc aacacgcatc cggcgcaggt gttcatgggc gacgtgggtg
     7741 cgctggcact gggcgcggcg ctggggacca tcgcggtgat cgtgcgccag gagatcgtcc
     7801 tggccatcat gggcggcgtc ttcgtggtcg aggccatctc cgtcatggcg caggtgatgt
     7861 acttcaagta cacgaagaag cggttcggcg aaggccggcg catcctgaag atggcgccgc
     7921 tgcaccacca cttcgaaaag agcgggtgga cggagaccca ggtggtcgtg cgcttctgga
     7981 tcatcaccat gctcctgtgc ctggtcggct tgtcgaccct caagctgagg taaccgtgca
     8041 cgagctcaag gaccggcacg ttctcatcct cggcctcggc gcgtccgggc tggcgatggc
     8101 gcgctggtgc gcgcgctgcg gcgccatggt gacggtggcg gacacgcgcg aggatccgcc
     8161 gcaattgcag gcgctgcgcg agcagttgcc gcaggcgcgc ttcaccggcg ggcctttcgg
     8221 cgccgatctg gtatcggagg aagtgcaggc ggtgtttcgc agccccggcc tgtcgccaga
     8281 gcaggtggcg gttgtcgcct gcgcggctgg tgagcgcggt accccggtcg gcggcgagct
     8341 gagcctcttc gcggatgccc tggccggcct gcgcgcgcgc gagggttatg cgcccacagt
     8401 gctcgcggtg accggtacca acggcaagac caccgtcacg gcgctggcgg gccagctcgt
     8461 cgagcgctcg ggacggactg tcgcggttgc cggcaacatc ggtcccacac tgctcgatac
     8521 gctggccggc aagatcgact cgggcgcctt gccgcaggtt tgggtgatcg aactctcgag
     8581 tttccagctg gacacgccgg gcgaattcga gtcgacggcc gcggccgtgc tgaacgtgac
     8641 gcaggatcac ctggactggc acggcagcat ggaagcctac gccggggcga aggcacgcat
     8701 cttcggcaag aacgcactga tgatcctcaa tcgcgaggac ccgctggtga tggggatggt
     8761 gccggagccc attcgggtga aaggcggcag gatggagcag cgcccgtacc tcacgttcgg
     8821 gggcgacgcg ccgcagcgtc ccggcgattt cggcatcgag gtcgtcaacg ggatggcctg
     8881 gctggtgcgc gcgcaggaag cggacgagac gcagaggcgc cggccgcgct cgctccaggc
     8941 gcgcgagctc gctgccgccc aggccggcgc ggacgaggaa ctctacatcc agcggctgat
     9001 gccggccgac gccttgcgca tccgcggccg ccacaatgcc gtcaacgcac tgtcggccct
     9061 ggccctggcc ggcgccgccg gctgcccgct cgggcccatg ctctacggcc tgcgcgaata
     9121 ccgcggcgag ccgcaccggg tggagccggt gggcatcatc aaccaggtcg agtatttcga
     9181 cgacagcaag ggcacgaacg tcggcgcgac ggtcgcggcc ctgcaaggcc tgggcgccga
     9241 gcgcaaggtg atcgtcattc tcggcggcga cggcaagggg caggacttct cgccgctggc
     9301 cgcgccggtc tcgcgtcatg cccgggccgc cgtgctgatc ggccgggatg cgcccctgat
     9361 ccgcgccgtg ctggagccgg ccggcgtgcc gctgatcgac gccggatcga tgcgggaggc
     9421 cgtgtcgctg gcaaccgaaa gggccagccc cggcgacgcc gtgctgatgt cgccggcctg
     9481 cgccagcttc gacatgttca ggaactaccc gcatcgcgcc gaggtcttcc gcgccgccgt
     9541 gcaaggcatc gccgacgagg cgggcgtgca gctggagggc gcggcatgag cgcgaagggc
     9601 gaggcagcgc cggaacgtgg cgcctcggca cgtgcgctgt ggaggcgctg cgacgattcg
     9661 gctgcgcgga gcagctgcgc aggctctgcc gctccgccga atccccgcgt gcggggatcg
     9721 accagcgcga atgccgcctg tcgccgaccc gcgggcgctg gcacccaatg cttgtccgga
     9781 ggcgccgcat gagcctgcag gctgttattg cgggcctgat ccccaatccg ttggcttcaa
     9841 ctgcgaaacc gatccggacg cggcgcgaca ggcacctggg caagcccgca tccgtgcgcg
     9901 tccagggcgt cgaccagccg ctgctgtggg tcacggttgc gctgctcgcg tttggcctgg
     9961 tgatggtgta ttcggcgacg gtggccctgc ccgacaaccc caagttcgcg cgctacagcc
    10021 acacctactt cctcgtgcga cacatcgttt tcctggcgct cggcctcgtg gccgcgctga
    10081 tggccttcca ggttcccatc gccacctggg aaaaaatcgc gccgtggcta ttcgtcgcat
    10141 cgctggtgct gttgatgctg gtgctgattc cgggtgtcgg atccgtcgtc aacggcgccc
    10201 ggcgctggat ctcggtgggt ttcatgggct tccagccgtc cgagctcgcc aagttcgcgg
    10261 tgctgctgta cgcggccgat tacatggtgc gcaagatgga cgtgaaggag cggttcttcc
    10321 gcgcggtgct gccgatggcc gctgccgtcg ccgtggtggg gctgctgctg ctggccgagc
    10381 cggacatggg cgccttcatg gtgatcgcag tgatcgcgat gggcatcctg ttcctgggcg
    10441 gcgtgaacgc ccgcatgttc ttcctgatcg cctcggtgat caccatcgcc ttcgtgctga
    10501 tgatcgcgct gtccgactgg cggcgcgagc gcatcttcgc ctacctcgac ccctggagcg
    10561 aaaagtacgc gctgggcaag ggctaccagc tttcccattc gctgattgcg atcggccgcg
    10621 gcgagatctt cggcgtgggc ctgggcggca gtgtggagaa gctgcactgg ctgcccgaag
    10681 cgcacaccga tttcctgctg gcggtgatcg gcgaggagtt cggccttatc ggcgtcgtca
    10741 ccgtcatcgt cgcgttcctg tggatgaccc ggcgcatcat gcacatcggc cgccaggcca
    10801 tcgcactcga ccgtgttttc gcggggctgg tggcacaggg cgtgggcatc tggatcggct
    10861 tccaggcttt catcaacatg ggcgtgaacc tcggtgcctt gcccaccaag ggcttgaccc
    10921 tgcccttgat gagctatggc ggttcggcca tcctgatgaa cctggtggcg atcgcggtgg
    10981 tgctgcgggt ggactatgaa aacaaagtcc tgatgcgcgg aggccgcgca tgaagacttt
    11041 gtttcgccaa aacaaaagtc agcgccttgc gctgcaatcc gcaatgcgcg gattggctga
    11101 tgcgcggagg cagaccatga gcgcccgccg gtgttttggt cccctctccc tctgggagag
    11161 ggttagggtg agggcacctg cgccgcacca agccctcacc cctgccctct cccaaaggga
    11221 gagggggaaa gagcgcgcgg catgacctct gcaaaatgcg cactcgtcat ggcgggcggc
    11281 acaggcggcc acatcttccc cgggctggcg gtggccgagg cgctgaggga gcgggcctgg
    11341 cgcgtccact ggctgggcgg gtcggccagc atggaaagcc aattggtgcc gccgcgcggg
    11401 tttgcgttcg agaccatcga cttttcgggg gtgcggggca agggcgtcaa gaccctggtg
    11461 ctgctgcccc tgcgactgct gagggcgttc tggcagagca tcgccgtggt ccggcgtgtg
    11521 aagcccgacg tggtgatcgg tctgggaggc tacatcacct tcccggccgg catgatgagc
    11581 gtgctgctgg gcaagccgct ggtgctgcac gagcagaact cggtggccgg catggccaac
    11641 cggatcctgg cgggcgtggc cgaccgggtc ttcacggcat tcccccacac gctcggcaag
    11701 gcggcctggg tcggcaatcc actgcgcgcg gccttcctgc agcagccggg cccggcggag
    11761 cggatggccg cgcgcgaggg gccgttgcgc ctgttggtcg tgggcggcag cctgggggcg
    11821 cgggcactca acgacatcgt cccgaaggcg ctcgcgctga ttcccgcgaa cgagcgcccc
    11881 gtggtgctcc accaaagcgg ggccaagcag atcgacgagc tgcgcgccaa ctatgccgcc
    11941 gccggcgtgc aggccgaact cacgcccttc atcgaggaca ccgcgcgtgc cttcgccgag
    12001 gccgacctga tcgtctgccg tgccggcgcc agcaccgtga ccgagatcgc ggcggtgggc
    12061 gcggcggcgc tgttcgtgcc gttcccctcg gccgtggacg accaccagac caccaacgcg
    12121 aagttcctgg tcgacgcggg cggcggctgg ctggtgcagc agcgcgacct gagacccgaa
    12181 tggctcgccc atctggtgcg cactaccgga cggccggagc tgatccgaag ggcgctggcc
    12241 gccaggagca tgagcaagac cagcgccacg gccgaagtgg tcaaagcctg cgaggagttg
    12301 gccaaaccat gaaacacgcc gtcaagcaca tacatttcgt cggcgtgggc ggcgccggca
    12361 tgtccggcat cgccgaagtg ctgcgcaatc tcggttacct gatctcgggc tcggacctcg
    12421 ccgacagccc gacgctgcgg cggctggaag cgctgggcat caagacctac ctgggccatg
    12481 cggcgagcca cgtgatcggc gccgacgcgg tggtcacttc gaccgcggtg caagccgaca
    12541 accccgaagt gctggccgcg cgccagcgca agatcccggt ggtgccgcgc gcgatgatgc
    12601 tggccgagct gatgcgcctg aagcagggca tcgccatcgc aggcacgcac ggcaagacga
    12661 cgacgaccag cctggtggcc agcgtcctgg ccgagggcgg gctggacccg accttcgtga
    12721 tcggaggccg gttgaacagc gcgggcgcca acgccaagct gggtggcggc gactacatcg
    12781 tggtcgaagc ggacgagtcg gacgcctcct tcctgaacct catgccggtg atggcggtgg
    12841 tgaccaacat cgacgccgac cacatggaaa cctacggcca cgacttcaac aagctcaagg
    12901 gtgcgttcct ggatttcctg caccgcatgc ctttctacgg cacggcgatt ctctgcatcg
    12961 acgacccggc ggtgcgcgag atcgtgccgg acgtgcaatg cccgatcacg agctacggcc
    13021 tcggcgagga agcccaggtg cgcgccatcg atgtccgggc cgtcggaacc cagatgcatt
    13081 tcaccgtgca gcggcgcaac ggcgtgtccc tgcccgacct gaaggtggtg ctcaacctcg
    13141 ccggcatgca caacgtgctg aacgccctgt cggcgatcgc cgtggcggtg gaactcaaca
    13201 tccccgacca agcggtgctc aaggcgctgg cggagttcac gggggtgggc cggcgtttcc
    13261 agaactatgg ccaggtggcc gtgctgcccg agaacggcgg cggccatttc acggtgatcg
    13321 aggactacgg tcaccacccg gtggaaatcg tggcgacgct gtcggcggca cgcggcgcgt
    13381 tcccgggccg gcgtctggtg ctcgcgttcc agccgcaccg ctacacgcgc acgcgcgatt
    13441 gcttcgagga tttcgtgaag gtgctcggcc acgccgacgc cgtgctgctg gccgaggtgt
    13501 attcggccgg cgaggcgccc atcgtggcag ccgatggccg gtccctggca cgcgcggtgc
    13561 gcgtggccgg caaggtcgag cccatattcg tcgaccccat cgatgccatg cccctggcca
//
LOCUS       DASWIY010000157        14455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2319880, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000157 DASWIY010000000
VERSION     DASWIY010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 14455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14455
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2319880"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..674
                     /gene="greA"
                     /locus_tag="VGA59_05925"
     CDS             198..674
                     /gene="greA"
                     /locus_tag="VGA59_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX9719251.1"
                     /translation="MATIPITRRGAEKLRSELHRLKTVDRPGVITAIADARAQGDLSE
                     NAEYEAAKDRQGFIEGRIKEIESKLAAAQVIDPTTVHAGGKVVFGATVELEEEDSGDK
                     VKYQIVGEDEADLKHGLINISSPIARALIGKEEGDTAEVLAPGGLKRYEIVAVHYL"
     gene            720..1181
                     /locus_tag="VGA59_05930"
     CDS             720..1181
                     /locus_tag="VGA59_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX9719252.1"
                     /translation="MAAGLRRLGARWKDQVPVFAAALWWGSLTATGFVMVPTLFAHFA
                     TPAQAGQAAARLFTAQTWIAVGCGLLLLFASRQGHEAARMDWARGALIFVFAGLLLAL
                     VAEFAVSPRIMTRANLKLWHSVGSGMYVLQWVCAGVVLWKVADLKGSSSRA"
     gene            complement(1198..1671)
                     /locus_tag="VGA59_05935"
     CDS             complement(1198..1671)
                     /locus_tag="VGA59_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEX9719253.1"
                     /translation="MPAIQLTIAQRKEHRAQAHHLAPVVMIGNEGLTPAVKKETDAAL
                     KSHGLIKIRVLGDDRAQREAMFQELADQLNAAPIQHIGKLLVLWRPKPPKAKDEDEER
                     MPGPRDFKVLKYSKRGGQRPEVKTLRVLGNQRLTPGGQVKRAKPRQKSIKKGRHT"
     gene            1714..2376
                     /locus_tag="VGA59_05940"
     CDS             1714..2376
                     /locus_tag="VGA59_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX9719254.1"
                     /translation="MKIKTKSRKVNKAWLNDHVNDPYVKLAQKEGYRARAAFKLKEID
                     EALGLVKAGHLVVDLGSAPGAWSQYLRRKLSPGGAAAGELNGTIIALDVLPMEPVEGV
                     IFIQGDFREPEVLAQLEQAMSGRKADVVVSDMAPNLSGIESADAARIAHLVELAVEFA
                     VNHMKPEGALVTKVFHGSGYSQLVKQFKETFRTVKPLKPKASRDKSSETFLVGLGLKA
                     GK"
     gene            2504..4417
                     /gene="ftsH"
                     /locus_tag="VGA59_05945"
     CDS             2504..4417
                     /gene="ftsH"
                     /locus_tag="VGA59_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX9719255.1"
                     /translation="MNNQWFSKIAVWLVIALVLFTVFKQFDTRGVAGASVMGYSDFLE
                     EVRSKRIKNAIIQEGQGGTEIVAITTDDRKIRTTATYLDRGLVGDLISNDVKFDVKPR
                     EEGSLLMTLLVSWGPMLLLIGVWIYFMRQMQGGGKGGAFSFGKSKARMLDENNNQVTF
                     ADVAGCDEAKEEVKEVVDFLKDPQKFQKLGGRIPRGLLLVGPPGTGKTLLAKGIAGEA
                     KVPFFSISGSDFVEMFVGVGAARVRDMFENAKKNAPCIIFIDEIDAVGRQRGAGLGGG
                     NDEREQTLNQMLVEMDGFETNLGVIVVAATNRPDILDAALLRPGRFDRQVYVQLPDIR
                     GREQILNVHMRKIPIGQDVNASIIARGTPGMSGADLANLCNEAALMAARRNARVVEMQ
                     DFEKAKDKIFMGPERKSMVMPEEERRNTAYHESGHALIGKLLPKCDPVHKVTIIPRGR
                     ALGVTMSLPAQDRYSYDREYMLNQIAMLFGGRIAEEVFMKQMTTGASNDFERATTLAR
                     DMVMRYGMTEALGPMVYAENEGEVFLGRSITKTTNMSESTMQKVDQEVRRIIDEQYAL
                     ARKLIEDNQEKMHAMAKALLEWETIDAEQLDDIMAGKDPRPPKDFTPRIPPSGSDGSG
                     GSPVAADPAPTAA"
     gene            4633..5490
                     /gene="folP"
                     /locus_tag="VGA59_05950"
     CDS             4633..5490
                     /gene="folP"
                     /locus_tag="VGA59_05950"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9719256.1"
                     /translation="MHWQTSRYLIDLSQPRVMGIVNVTPDSFSDGGRYAGSRSAVAHC
                     EQLVSEGADILDIGGESSRPGTPPVALEEELGRVLPVLRIAVTLGVPVSVDTCKPEVM
                     RQALDLGADIVNDIWALRQPGARELIAAHPSCGICLMHMHGEPRTMQRSPMEGDAVPQ
                     VLAFLERASLALRELGVDRGRIAWDPGIGFGKTVEQNFALLARQHVLLAAGYPLLVGW
                     SRKSSLGSVTGLPINDRLAASVAAALLAVDRGAHVVRVHDVKETVAALKVRGATISMQ
                     ERKNTETNP"
     gene            5487..6821
                     /gene="glmM"
                     /locus_tag="VGA59_05955"
     CDS             5487..6821
                     /gene="glmM"
                     /locus_tag="VGA59_05955"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX9719257.1"
                     /translation="MSRTYFGTDGIRGTVGEPPITPDFVLRLAHSVGRVLKRSEQRPT
                     VLIGKDTRISGYMLESALESGFNSAGVDVVLLGPLPTPGVAYLTRAQRASLGVVISAS
                     HNPYPDNGIKFFSSQGTKLPDEWEQAVEAALREPPAWSDSAALGKARRLDDAPGRYIE
                     FCKSTFANDLTLKGMTIVVDAAHGAAYHIAPKVFHELGAEVICIGCSPDGLNINDNVG
                     ATHPDALVRAVQASHADIGIALDGDADRLQLVDAAGRLYNGDELLYLMAVDRLDRGQP
                     VPGVVGTLMTNMAVELALKARGVDFVRTRVGDRYVLEELEKRGWLLGGEGSGHLLVLD
                     QHTTGDGLISALQVLKANVRSGRSLAQLLQGVTLFPQTLINVRLPPGQDWKENRALAQ
                     ETQRAEAELDGHGRVLIRASGTEPVLRVMVEAREETQARDTAERLAAAARAE"
     gene            6932..7444
                     /locus_tag="VGA59_05960"
     CDS             6932..7444
                     /locus_tag="VGA59_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719258.1"
                     /translation="MRSVLMLIRWSPLVAGWVLAWPNAATAQTFSADYALARSGIEAP
                     PGPHHLGLISFGRVRVGPALHSDGDFAGGGLSVTAGQNWFGGVAIGRNLQSGPDGMAG
                     QGAVRIGGGYRWSDGQALSLQLTGPRSGNRLGLSVNYDWPRYFVRLSYDAGLNPLPQD
                     KLRFSAGMRF"
     gene            7643..10711
                     /locus_tag="VGA59_05965"
     CDS             7643..10711
                     /locus_tag="VGA59_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX9719259.1"
                     /translation="MASVSPSQRNPRLLIVSVLAAILVGGIYWVAYELRTSQLQARLF
                     TKFARELEFKVGAGPTHDIRFPTHGPYDKRLGYQQLPAMIDGLTGEGFEVTAQARMSP
                     RLLALTERGISPPYREKDQAGLEILDCRARPLFRARVPERVYERFEDVPSLLVDALLF
                     VENRGLLDPERAMLNPAVDGGRLLKAVFDRALRVVDRGHASPGASTLATQIEKFRHSP
                     EGRTESVSEKLRQMTSASLRAYIDGKDTMPRRQEIIRSYLNTVPLAAQAGFGEVNGLG
                     DGLWAWYGRDFAEVNRLLMTYRDGDIFPAPLRERRRALAFKQALFFKQAEAYKQALSL
                     MIAQRRPSDYLANGAPSLGALTDSYLRVMAAAGVISPVLRDAALQAPLRLRVGPVAGP
                     ANSFVDHKAATAVRATLSRILDTPLAYDLDRIDLVARSSIDGEAQQTASRMLRSLRDP
                     AAARAAGLYGFHLLSSGDDPSKLSFSFTLLERGDTANLLRVQTDSSEQPFDINEGARL
                     DLGSTAKLRTLITYLEVVSEFHARWSTLESGALAMHKADNDNPLGRWALDYLSRASDR
                     SLAAMLDAAMNRRYSASPAEAFFTGGGLHRFANFEPKDNGRVLTVREALARSVNLVFI
                     RLMRDLVRHYMFGISHSASSILRDQADPRRRDLLVRFADKEGRQFLARFHAKYMGKSP
                     REAQELLLRGRRATPARLASILFALEPEAGTDALKTFLSQRSTGAGLSERSIRALFDK
                     YGPGRLPLADRSYIAGVHPLELWLVGFLRAHPRATLGDVVAASQSIRQEAYGWLFKSS
                     KKRAQDVRIRSMLEFDAFGEIQRAWKRLGYPFDALTPSYAAAIGASGDRPAALAELIG
                     IIVSRGRRLPVAKLESLEFAADTPFETRLRYRPAQAERVLPEEVAATVRRALVDVVQD
                     GTARRLRGVLGRPDGSRFDVGGKTGTGDHRIEVYGAGGRLVASRIVDRSATFVFLIGE
                     KYFGTVMVYAHEPFAAKYRFTSALPVQLLKSLLPVILPMLEQGACSRAGN"
     gene            complement(10859..11302)
                     /locus_tag="VGA59_05970"
     CDS             complement(10859..11302)
                     /locus_tag="VGA59_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX9719260.1"
                     /translation="MKSQLALFAALLLPLWGHTVQAREMVSVDRAEINMRAGAGTGHA
                     TLWALIRGYPLVVTGRKGQWLKVRDFENDTGWVYGPLVGNKPHHVVKARVANIRSAPS
                     TSSRVVGKAERGEVLRTVERRNSWVKVQQEGGLKGWVARRLVWGW"
     gene            complement(11417..13690)
                     /locus_tag="VGA59_05975"
     CDS             complement(11417..13690)
                     /locus_tag="VGA59_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HEX9719261.1"
                     /translation="MNATIKPEHLALRGDNSEDYNTSPNPSFNEVLDARLSRRNVLRV
                     SLGTAGAAVLGALPLAGCGGSSDASTEGTTASTATELQLSFPTVAKSTADAVAVPTGY
                     TATVLYALGDPLTATTPDFKNDGTDTGFDDRSGDHHDGMEYFGLNAAGTARDVAGSER
                     ALLVINHEALTDQFLHANGTTANPRPAAEADKEIPAHGVSIVEIRKVGGKFATQKDSA
                     YNRRITPMTPFQINGPARGSALMKTKFSAPGTDGRGTINNCGTGYTPWGTFLTGEENW
                     SGYFRRGDDEAVRGGATARANVSLARYGRAKNSASRHGWETAGSDDKYARWDITQAGV
                     STDGTDDYRNELNTYGYIVEIDPYDKNMAIRKRTALGRFAHESAAFGKLVAGKPLAVY
                     MGDDSRGEYIYKFVSGANWAAGDASPANRITTGDKYLDSGKLYVAKFNPDGTGQWIEL
                     AIGNAAIADYAKYDFADQADVVVNARLAADAVGATKMDRPEWCAVHPVTGEVYYTLTN
                     NSNRKVEPSSSSQMAVDAANPRSYTDAPTATTSPGNVNGHIIRMKENAGESAATAFVW
                     DVYLFGAQADADLARVNLSALTADQDFSSPDGLWFSPKTGLCFIQTDDGAYTDVTNCM
                     MLIGKPGAVGDGTTASLTYTKTDGSTVTVQTRVGAKPTPATLRRFLVGPVDCEITGIA
                     ETPDGKAIFVNVQHPGEAISKANVADPAKYLSHWPGNAGYGAGGAAARPRSATIVITR
                     NDGGLIGADGTAVLNAY"
     gene            13817..13945
                     /locus_tag="VGA59_05980"
     CDS             13817..13945
                     /locus_tag="VGA59_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719262.1"
                     /translation="MGRMNIRMPHPIEVLALCAAVVRGLAEFVSLQRWRLRERRLR"
     gene            14038..>14455
                     /locus_tag="VGA59_05985"
     CDS             14038..>14455
                     /locus_tag="VGA59_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX9719263.1"
                     /translation="MAAEGLTLPVYTVALGNPGAASAAGFFGGVHGLERIGSDVVTTW
                     LHSLVMRLRWDSSLHTLMERVRLVFMPIVNPGGMLRGTRANLNGVDLMRNAPVDAQDG
                     VPFLVGGQRISAGLPWYRGRRGDAMETESAALCELVE"
BASE COUNT         2426 a         4558 c         4860 g         2611 t
ORIGIN      
        1 ttcacgacca tcgccggcgc cgaagcggcc gtggaaggga tgaagtacct ggacaacctg
       61 gacgtctatt cattgcagga gctgcacgcc caactggctt gaactcgacg gcgcgccgtc
      121 agtcccctta taatcgcggc atcgagagca ccgccggtcg gcaaggtcgg cggtgttcct
      181 ttttctggag cattcagatg gcgacgattc cgatcacgcg gcgaggagcc gaaaaactca
      241 ggtccgagct gcaccggctc aagacggtgg accgcccggg cgtgatcacc gcgatcgccg
      301 atgcgcgggc gcaaggggat ttgtccgaaa acgccgagta cgaagccgcg aaggaccgcc
      361 aggggtttat cgaagggcgg atcaaggaga tcgaaagcaa gctcgcggcc gcccaggtca
      421 tcgacccgac gaccgtgcac gcgggcggca aggtcgtgtt cggggccacc gtggagctgg
      481 aggaggaaga ctcgggcgac aaggtgaaat accagatcgt cggcgaggac gaggccgacc
      541 tgaagcacgg cctgatcaac atctcgagcc ctatcgcgcg cgccctgatc ggcaaggaag
      601 agggcgatac cgccgaggtg ctggcgcccg gaggcctgaa acgctacgaa atcgttgcgg
      661 tccactacct ttagccttta gcaatggacc ccagcgggct tcgccctgcc cccaaagggg
      721 tggccgccgg tttgaggcgg ctcggtgccc gctggaagga tcaggtcccg gtttttgccg
      781 ccgcgttgtg gtggggcagc ttgacggcga cgggtttcgt gatggttccc acgctgttcg
      841 cgcactttgc caccccggcg caggccggcc aggcagcggc gcggctgttc accgcgcaga
      901 cctggatcgc ggtgggctgc gggctgctgc tgctgttcgc ttcaaggcag ggccatgagg
      961 ccgcgcgcat ggactgggcc cgaggcgccc tgatttttgt ttttgccggc ttgttgcttg
     1021 ccctcgtggc cgagttcgct gtctcgccca ggatcatgac gcgcgccaac ctcaagctgt
     1081 ggcacagcgt cggcagcggg atgtatgtgt tgcagtgggt atgtgcgggc gtggttctct
     1141 ggaaagtggc ggacctgaaa ggttcgtcat cccgggcttg acccgggatc caggtattca
     1201 ggtgtgccgg cccttcttga tcgacttctg cctgggtttg gcccgcttga cctgcccgcc
     1261 gggagtcagg cgctggttgc ccaggacgcg caaggtcttg acctcgggac gctggccgcc
     1321 gcgcttgctg tacttgagca ccttgaaatc gcgcggaccg ggcatccgct cctcgtcctc
     1381 gtctttggcc ttgggcggct tgggccgcca gaggacgagc agcttgccga tgtgctggat
     1441 cggagcggcg ttcagctggt cggccagttc ctggaacatg gcttcgcgtt gcgcccgatc
     1501 gtcgccgagc acgcggatct tgatcaggcc gtgggacttg agggcggcgt cggtttcctt
     1561 tttgacggcg ggggtgaggc cctcattgcc gatcatgacg accggggcga gatggtgagc
     1621 ctgcgcgcga tgttccttgc gctgggcgat agtcagttgg atagcaggca tggggactat
     1681 tatggctttg gccgaccggg caagaatcga agcatgaaga tcaagacgaa aagcaggaag
     1741 gtcaacaagg cgtggttgaa cgaccacgtc aacgacccct acgtcaagct cgcgcagaag
     1801 gagggttacc gcgcgcgcgc cgccttcaag ctgaaggaaa tcgacgaggc cctcggcctt
     1861 gtaaaggcgg gccacctggt ggtggacctc ggttcggcgc cgggcgcctg gagccagtac
     1921 ctgaggcgca agctctcgcc gggcggcgcg gcggccggcg agctcaacgg gaccatcatc
     1981 gcgctggacg tcctgcccat ggagccggtc gagggcgtca tcttcatcca gggggacttc
     2041 cgcgagccgg aagtgctggc tcagctggaa caggccatgt cggggcgcaa ggccgacgtc
     2101 gtggtatcgg acatggcccc caacctgtcg ggcatagagt ccgccgacgc cgcccgaatc
     2161 gcgcacctgg tcgagctggc tgtcgaattc gcggtcaatc acatgaaacc cgagggagcc
     2221 ctggtaacca aggtgtttca tggaagcggc tacagccagc ttgtcaagca gttcaaggaa
     2281 actttccgca ctgtcaagcc tcttaaacct aaggcttcgc gcgataaatc atctgagact
     2341 ttcctcgtgg gcctcgggtt gaaagcaggg aagtgaggcg agctcgagct tgttcctcgg
     2401 tcgcccggcc actatgccag ccgtagcgaa aatacatcat tccacggctt gaaacgccta
     2461 gaatggacag caactagcac aagtaccctc ccggagactg aagttgaata atcagtggtt
     2521 ttccaagatt gccgtgtggc tcgtcatcgc cctcgtgctg tttaccgttt tcaagcagtt
     2581 cgacacgcgc ggcgttgccg gggccagcgt gatgggctat tccgatttcc tcgaggaagt
     2641 tcgcagcaag cgcatcaaga acgccatcat ccaggagggg cagggcggca cggaaatcgt
     2701 cgccatcacc accgatgacc gcaagatccg cacgacggcc acctacctcg accggggcct
     2761 ggtcggcgac ctcatcagca acgatgtcaa gttcgatgtg aagccgcgcg aagaaggctc
     2821 gctcctcatg acgctgctgg tcagctgggg gcccatgctc ttgctgatcg gagtctggat
     2881 ctacttcatg cgacagatgc agggcggcgg gaagggaggc gccttcagct tcggcaagag
     2941 caaggcgcgg atgctggatg agaacaacaa tcaggtgacc ttcgcggatg tagccggatg
     3001 tgatgaagcg aaagaggagg tgaaagaagt cgtcgacttc ctgaaggatc cgcagaaatt
     3061 ccagaagctg ggcgggcgca tcccgcgcgg cctgctgctg gtcggccccc cgggcacagg
     3121 caagacgctg ctggccaagg gcattgccgg cgaggccaag gtcccgttct tcagcatctc
     3181 cggttccgac ttcgtcgaaa tgttcgtcgg cgtgggcgcg gcccgtgtgc gcgacatgtt
     3241 cgagaacgcc aagaagaacg cgccctgcat catcttcatc gatgaaatcg atgccgtcgg
     3301 ccgccagcgc ggcgccggcc tgggcggcgg caacgacgag cgtgaacaga ccctgaacca
     3361 gatgctggtc gagatggacg ggttcgagac caacctgggc gtgatcgtgg tcgcggccac
     3421 caaccggccc gacatcctgg acgccgcgct gctgcgcccg ggccgcttcg accgccaggt
     3481 ttatgtccag ctgccggaca tccgcggccg cgagcagatc ctgaacgtcc acatgcgcaa
     3541 gatccccatc ggccaggacg tcaacgccag catcatcgcc cgcggcacgc ccggcatgtc
     3601 gggcgccgac ctcgccaacc tttgcaacga agccgccctg atggctgcgc gccgcaacgc
     3661 gcgcgtggtc gagatgcagg atttcgaaaa ggccaaggac aagatcttca tgggccccga
     3721 gcgcaagagc atggtcatgc ccgaggaaga gcgccgcaac acggcctacc acgagtccgg
     3781 ccacgccttg attggcaagc tcctgcccaa gtgcgacccg gtgcacaagg tcaccatcat
     3841 cccgcgcggc cgtgcgctgg gcgtcacgat gagcctgccg gcgcaggacc gctacagcta
     3901 cgaccgcgag tacatgctca accagatcgc catgctgttc ggcggccgga ttgccgaaga
     3961 agtgttcatg aaacagatga ccaccggcgc ttcgaacgat ttcgagcgcg ccacgacgct
     4021 ggcccgcgac atggtgatgc gctacggcat gaccgaggcc ttgggcccga tggtctatgc
     4081 cgagaacgag ggcgaggtgt tcctgggccg ctcgatcacc aagaccacca acatgagcga
     4141 atcgaccatg cagaaggtcg accaggaggt ccgccgcatc atcgacgagc agtacgccct
     4201 ggcgcgcaag ctgatcgagg acaaccagga aaagatgcac gccatggcca aggccctgct
     4261 ggagtgggaa accatagacg ccgagcagct ggacgacatc atggccggca aggatccgcg
     4321 cccgcccaag gacttcacgc cgcgcatccc gccgtccggc agcgacggtt cgggcggttc
     4381 accggtggcg gccgatccgg cgccgacggc ggcgtaagga tttgtcattg cgggccgggc
     4441 tcgcggaacg cccggggcgc gcagctgtcc agtgtagcga gcccattgcc gggcctacgg
     4501 tatgcacgta ccgaggcatg acgctccggc ccggccccgc aatccacgct ggcgtgttga
     4561 cgcactcttg cccgtgaggc atggatgccg ggtcaagccc ggcatgacaa gcgcttgtcg
     4621 caaaatcacc ccatgcactg gcaaacctcg cgttacctga tcgacctgtc ccagccccgg
     4681 gtcatgggca tcgtcaacgt cacgcccgac tctttttccg acggcggccg gtatgccggc
     4741 tcgcgctcag cggtggctca ttgcgagcag ctcgtctccg agggagcgga tatcctggac
     4801 atcggcggcg agtcgagccg cccgggcacc ccgccggtcg cgctggagga agagctgggg
     4861 cgtgtgttgc cggtgctgcg catcgcggtg acgctgggtg ttccggtttc ggtggatacc
     4921 tgcaaaccgg aggtcatgcg ccaagcgctc gacctggggg ctgatatcgt caatgacatc
     4981 tgggcgctgc ggcagcccgg cgcgcgtgag ctgatcgcgg cacacccctc ttgcgggatc
     5041 tgcctgatgc acatgcatgg cgagccccgc accatgcagc gctcgccgat ggaaggcgac
     5101 gcggtgcccc aggtgctggc cttcctggag cgggcgtcgc tggcgctgcg ggagctgggc
     5161 gtggaccgcg gccgcatcgc ctgggacccc gggatcggct tcggcaagac tgtcgagcag
     5221 aacttcgccc tgcttgcccg gcagcatgtg ctgctcgcag ccggctatcc tctgctcgtc
     5281 ggctggtcgc gcaaatcctc gctgggcagc gtgaccgggc ttcccatcaa tgacaggctg
     5341 gctgcgagcg tcgcggcggc actgctggcg gtggaccgcg gcgcccacgt cgtgcgggtg
     5401 cacgacgtga aggaaacagt ggcagcgctg aaggttcgcg gcgccacgat ttcgatgcag
     5461 gaaagaaaaa acacggagac gaatccatga gcaggaccta tttcggcacc gacggcatcc
     5521 gcggcacggt gggcgagcca ccaattaccc ccgatttcgt gctgcggctc gcccattccg
     5581 tgggccgcgt cctcaagcga tccgagcagc ggcccacggt cctgatcggc aaggacaccc
     5641 gcatctcggg ctatatgctc gaaagcgcgc tcgagtcggg cttcaactcg gccggtgtcg
     5701 acgtggtgct gctcgggccg ctgccgacgc cgggcgtcgc ctacctgacc cgggcccagc
     5761 gtgccagcct gggcgtggtc atcagcgcca gccacaaccc gtatccggac aacggcatca
     5821 agttcttcag cagccagggc accaagctgc cggacgagtg ggagcaggcg gtcgaagccg
     5881 cgctgcggga gccgcccgcc tggtcggatt ccgccgccct gggcaaggcg cggcgcctgg
     5941 acgacgcccc ggggcgctac atcgagtttt gcaagagcac cttcgccaac gacctcacgc
     6001 tcaaagggat gaccatcgtg gtggacgcgg ctcacggcgc cgcctaccac atcgcgccca
     6061 aggtcttcca tgaactgggc gcggaggtca tctgcatcgg ctgttcgccg gacggcctga
     6121 acatcaacga caacgtcggc gccacccatc ccgatgcgct ggtccgcgcc gtgcaagcga
     6181 gccatgccga catcggcatt gcgctcgacg gggacgcgga ccggctgcag ttggtggatg
     6241 ccgccgggcg cttgtacaac ggcgacgaac tgctctacct gatggcggtc gaccggctgg
     6301 accgcgggca accggtgcct ggcgtggtcg gcacgctgat gaccaacatg gccgtggagc
     6361 tggccctgaa ggcgcgcggg gtggatttcg tgcgcacgcg ggtcggcgac cgctatgtcc
     6421 tggaagagct cgaaaagcgc ggctggctgc tcggtggcga aggctccggg cacctgctcg
     6481 tgttggacca gcacaccacg ggcgacggcc tgatcagcgc cttgcaggtg ctcaaggcca
     6541 acgtgcgcag tggccgcagc ctggcgcaat tgctccaagg tgtgacgctg ttcccgcaga
     6601 ccttgatcaa tgtccggctg ccgcccgggc aggattggaa ggaaaatcgg gcgctggcgc
     6661 aagagacgca acgtgccgaa gctgaactcg acggccatgg ccgtgtcctg attcgcgcca
     6721 gtggcaccga accggtgctg cgggtgatgg tggaagcgcg cgaggagacg caggcccgcg
     6781 acacggccga gcggctcgcg gcggcggcac gagctgaata gggccagcgc accgtcgcgc
     6841 ctgtccaacg gcggtgccgc tcgtcgcatc caaacgacac atcatccggg aaaagctggg
     6901 cgggaatggg tccaaggtct acattggact catgcgttcg gtcctcatgc tcatccgttg
     6961 gtcgccgctc gtcgcgggct gggtgctggc atggccgaac gccgcaacgg cccagacgtt
     7021 ctcggccgac tatgcgctgg ctcgctcggg gatcgaagcc ccaccgggtc cgcatcactt
     7081 ggggctgatc agcttcgggc gggtccgcgt agggcccgca ttgcacagtg atggtgattt
     7141 cgcgggcggg ggattgtccg tcaccgccgg gcagaattgg tttggcgggg ttgcgatcgg
     7201 gcgcaacctg caatccggcc ctgatggcat ggccgggcag ggtgccgtcc gcatcggggg
     7261 cggataccgg tggagcgatg gacaagcgct gtccttgcag ctgaccggtc cgcgcagcgg
     7321 taatcgcctc ggcctgtccg tgaactacga ctggccgcgc tacttcgtgc ggctgtccta
     7381 cgacgccggg ttgaacccat tgccgcagga caagctgcgc ttctcggccg ggatgcgttt
     7441 ctagggcccg gggtcctggc tgcctcagta cgcgtctccg gcaatctctc cctcccgctg
     7501 taatcttgga ggcccgccgc gcagtcccct gccgtcaggc catttctttc gaccaatgat
     7561 ctactatggg ccagtgtgga gagcgcgaat gcgagctgcc ctcgagattg ggccgcgctc
     7621 ggcgagttga ggctttgacc ccatggcgtc ggtgtcccca tcgcagagga accccaggct
     7681 gctgatcgtc agcgttctgg ccgcaattct tgtgggcgga atctactggg ttgcatacga
     7741 gttgcggacg tcccagcttc aggcgaggct gttcaccaag ttcgcacgcg agctcgaatt
     7801 caaggtcggc gctggaccca cgcatgacat ccgattcccg acccacggac cttacgacaa
     7861 acgactcggc tatcaacagc tgccggcgat gatcgatggg ctgacaggtg agggttttga
     7921 ggtgactgcg caggcgcgga tgtcgccgcg gcttttggca ttgaccgagc gtggaatttc
     7981 tccaccctat cgcgagaagg accaggccgg gcttgaaatt ctcgactgcc gggcaaggcc
     8041 actgttcagg gcccgcgtcc ccgagcgcgt gtacgagcgc ttcgaggatg tgccgtcact
     8101 gctcgtcgac gcgctgttgt tcgtcgagaa ccgcggattg ctcgatccgg aacgtgcgat
     8161 gctcaaccct gcggttgacg ggggtcgtct gttgaaggcg gtcttcgatc gcgctttgcg
     8221 tgtcgtggac cgcggccacg cgtcgcccgg ggccagcacg ctggcgaccc agatcgaaaa
     8281 gttccggcac tcgcccgagg gtcgcaccga atcggtcagc gagaagcttc gtcagatgac
     8341 ctcggcttcg ctgcgcgcat acatcgacgg caaggacacg atgccgcgcc gacaagagat
     8401 cattcgcagc tacctgaaca cggtgccact ggcggcacaa gccggtttcg gggaggtgaa
     8461 tgggctgggc gacggactgt gggcatggta tgggcgcgac ttcgccgagg tgaatcggct
     8521 gttgatgaca taccgcgacg gcgacatttt cccggctcct ttgcgcgagc ggcgtcgggc
     8581 gctggccttc aagcaggcgc tcttcttcaa acaggccgaa gcgtacaagc aggcgctgtc
     8641 gctgatgatc gcccaacgtc ggccttcgga ctatcttgcg aacggcgcgc ccagcctggg
     8701 ggcgctgacc gacagctatt tgcgcgtcat ggcggccgcc ggcgtgatca gcccggtgct
     8761 gcgcgatgca gcgcttcagg cgcctttgcg tctgcgggtg ggccccgtgg caggaccggc
     8821 gaactcgttc gtagatcaca aggcggccac tgccgtgcgc gccacgctgt cgcgcatact
     8881 ggacacgcct ctagcctacg atctcgatcg catcgacctc gtggctcgaa gcagcatcga
     8941 tggggaagcg cagcagacag cctcgcgcat gctgcgcagc cttcgcgatc cggccgcggc
     9001 gcgggccgca gggctctatg gctttcacct gctcagcagc ggagacgatc cgagcaagct
     9061 ttccttcagc ttcacgctgc tcgaacgcgg ggacacggcc aacctgttgc gcgtccagac
     9121 cgacagcagc gagcagccgt tcgacatcaa cgaaggcgcg cgcctcgacc tgggatcgac
     9181 cgccaagctg cgcaccttga tcacctacct cgaagtcgtg tccgagttcc atgcccgctg
     9241 gagcacgctc gagagcgggg cgctcgccat gcacaaggcc gacaacgaca atcccctcgg
     9301 tcgctgggcg ttggactacc tgtccagggc ctcggaccgc agcctcgcag cgatgctcga
     9361 tgccgcgatg aatcgccgct attcggcgag cccggccgag gccttcttca cgggaggtgg
     9421 gctgcatcgc ttcgcgaatt tcgagccgaa ggacaacggc cgcgtcctga ctgtgcgcga
     9481 ggcgctggca cgatcggtga atctggtgtt catcaggttg atgcgcgacc tggtgcggca
     9541 ctacatgttt ggcatatctc actcggccag cagcatcttg agggatcaag ccgatccccg
     9601 gcgccgcgat ttgctggtcc gtttcgcgga caaggagggt cgccaatttc ttgcgcggtt
     9661 ccatgcaaag tacatgggca agtcaccgcg ggaagcccag gagctgctgc tgcgaggcag
     9721 gcgtgccacg ccggcacgtc tcgcgagcat tctgtttgcc cttgagccgg aggccggcac
     9781 cgacgcactg aagaccttcc tcagtcaacg atcgacaggc gccggcttgt ccgagcgttc
     9841 cattcgagca ctgttcgaca agtacgggcc tgggcgcctg cccttggccg atcgcagtta
     9901 tatcgcggga gtgcacccgt tggagctgtg gcttgttgga ttccttcgtg cccaccccag
     9961 agcgacgttg ggtgacgtag tggcggccag tcaaagcatt cgtcaggagg cctacggctg
    10021 gctcttcaag tccagcaaga agagggcgca ggatgtgcgc attcgcagca tgctggagtt
    10081 cgatgccttt ggcgagatcc agcgcgcctg gaagcggttg gggtatccgt tcgacgcgct
    10141 cacgccctcc tacgcggcgg cgatcggtgc atccggcgat cgaccagccg cgctcgccga
    10201 attgatcggc attatcgtta gtcgaggacg gcgcctgccc gtggcgaagc tcgagtctct
    10261 ggaattcgcg gcagacacac cgttcgagac acgccttcgg tatcggcctg cacaggcaga
    10321 gagggttctg ccggaagaag tcgccgcgac cgtccgtcgt gcgctcgtcg atgtcgtgca
    10381 agacggcacg gcgaggcgcc tgcggggtgt tcttgggcgg cccgatggga gcaggtttga
    10441 tgtgggcggc aagaccggca ccggtgacca ccgcatcgag gtgtacggag ccggtggtcg
    10501 attggtggcg agccgaatcg tagaccgatc ggccactttc gttttcctga tcggtgagaa
    10561 gtacttcggg accgttatgg tgtacgcgca cgagcccttc gcagcaaagt accgattcac
    10621 cagtgcgctg ccggtgcaac tgctgaagtc gctcctgccc gtgatcttgc cgatgctgga
    10681 gcagggtgcg tgcagtcgtg cgggaaactg acggcctacc tggatggagc agtttctact
    10741 atcgacgttg tggctcgccg taggatagat cgatcaggat cagctccatc gacgtcaaac
    10801 ttctgctgcc tccccggcag tggcgggacc cgttttggtg ctcgcgccgc cgcaggtctc
    10861 accagcccca caccaggcgg cgcgccaccc aacccttgag cccgccttcc tgctggacct
    10921 tcacccagct gttgcgacgc tctacggtgc gcagcacctc gccgcgttcg gcctttccca
    10981 caacacggct gctggtgctc ggcgcgctgc ggatgttggc cacacgcgcc ttcaccacgt
    11041 ggtgcggctt gttgcctacc agcgggccgt acacccagcc cgtgtcgttc tcgaagtcgc
    11101 gcaccttgag ccactggcct ttgcggccgg tgaccaccag cggataaccc ctgatcagcg
    11161 cccacagcgt cgcgtgcccg gtgccggcgc cggcgcgcat gttgatctca gccctgtcca
    11221 cgctcaccat ctccctggcc tgcaccgtgt gtccccacag cggcagcaac agggcggcaa
    11281 acaacgcgag ttggcttttc aatcgggaca tatggctcca atcaaggtca ggatcaaagt
    11341 ttaacaacac ttcgagcgcg caggccgcgg gcggtttgtc cggcagcggg acctcctagg
    11401 ccagggcctg gctgcctcag tacgcgttca gcaccgcggt cccgtcagcc ccaatcaggc
    11461 cgccgtcgtt gcgggtgatg acgatcgtgg cggaccgcgg tcgggccgcg gcgccgccgg
    11521 cgccgtagcc tgcattgccg ggccagtggc tcaggtactt ggccgggtcg gcgacattcg
    11581 ccttgctgat ggcctcgccc gggtgctgca cgttcacgaa aatggccttg ccgtccggcg
    11641 tctccgcgat gccggtgatt tcgcagtcca ccgggcccac gaggaatcgt cgcagcgtcg
    11701 ccggcgttgg cttggcaccc acgcgcgttt gcaccgtgac ggtactgcca tccgtcttcg
    11761 tgtaggtcag ggaggctgtg gtgccgtcgc ccaccgcgcc gggcttgccg atcagcatca
    11821 tgcagttggt gacgtcggta taggcgccgt cgtcggtctg gatgaagcac aggcccgtct
    11881 tggggctgaa ccacaggccg tccgggctgg agaagtcctg gtccgccgtc agggccgaca
    11941 ggttgaccct ggccaggtcg gcatccgcct gcgcgccgaa caggtacacg tcccagacga
    12001 aggccgtcgc ggcgctctcg ccggcgtttt ccttcatgcg aatgatgtgg ccgttgacat
    12061 tgccggggga cgtcgtcgcc gtcggcgcat ccgtgtaaga gcgcggattg gccgcatcca
    12121 ccgccatctg cgaggagctg gagggctcga ccttgcggtt gctgttgttg gtgagcgtgt
    12181 agtacacctc gccggtcacc gggtgcacgg cgcaccattc ggggcggtcc atcttggtcg
    12241 cacccaccgc gtcggcggcc aggcgggcgt tgaccaccac gtcggcctgg tcggcgaaat
    12301 cgtacttggc gtagtcggcg atcgcggcgt tgccaatggc cagttcgatc cactgaccgg
    12361 tgccatcggg attgaatttc gccacgtaga gtttgccgct atccaggtac ttgtctccgg
    12421 tggtgatgcg gttggccggg ctggcgtcgc cagcggccca gttcgcgccg gaaacgaact
    12481 tgtagatgta ctcgccgcgc gagtcgtccc ccatatacac ggccagcggc ttgccggcca
    12541 cgagcttgcc gaaggccgcg ctttcgtggg cgaagcggcc caatgccgtg cgcttgcgga
    12601 tggccatgtt cttgtcgtag ggatcgatct cgacgatgta gccgtaggtg ttgagttcat
    12661 tgcgatagtc gtccgtgccg tccgtcgaga cgccggcttg cgtgatgtcc cagcgcgcgt
    12721 acttgtcgtc gctgcccgct gtctcccagc catgccggct ggcgctgttc ttcgcccgcc
    12781 catagcgcgc cagggaaaca ttggcgcggg ccgtggcgcc gccgcggacc gcctcgtcgt
    12841 cgccgcgccg gaagtagccg gaccaattct cctcaccggt gagaaacgtt ccccaggggg
    12901 tgtagccggt gccgcagttg ttgatggtgc cgcggccgtc cgtgccgggg gcggagaact
    12961 tcgtcttcat caatgcgctg ccgcgcgccg gtccgttgat ctggaacggc gtcatggggg
    13021 tgatgcggcg gttgtacgcg gagtccttct gggtggcgaa cttgccgccg actttgcgaa
    13081 tctcgacgat ggatacgcca tgcgccggga tttccttgtc ggcctcggcg gcggggcgtg
    13141 gattcgcggt cgtgccgttc gcatgcagaa actggtcggt caacgcttcg tggttgatga
    13201 ccagcagcgc gcgctcggag ccggccacat cgcgcgcagt accggccgca ttcaggccga
    13261 agtactccat gccatcgtgg tggtcgcccg accggtcatc gaagccggtg tcggtgccgt
    13321 cgttcttgaa gtcgggggtg gtggcggtaa gcggatcgcc cagagcgtac agcacggtgg
    13381 cggtgtagcc ggtgggaacc gccaccgcgt cggccgtgct cttggcaacg gtgggaaaac
    13441 ttagctgcag ctcggtggct gtggaggctg tggtgccctc cgtcgatgcg tcactacttc
    13501 cgccgcagcc ggccaatggc agcgcaccca gcacggccgc gcccgccgtt cccaggctga
    13561 cgcgcagcac gttgcgccgg ctcagccgcg cgtccagcac ttcgttgaac gacggattgg
    13621 ggctggtgtt gtagtcctcg ctgttgtcgc cgcgcagggc caggtgctcg ggcttgatgg
    13681 ttgcgttcat ttcttcgatg cctttcgttg gttggtgctc tcaggtcagc cgccgagcgt
    13741 aggaaggatt cgtgacacgg gcgtgaatcc caatgtcctg gcaccagtca tcgttctgca
    13801 acacgaatga aggaacatgg gccggatgaa tatccggatg ccgcatccca tcgaggtgct
    13861 cgcgctctgt gccgccgtgg tgcgggggct ggccgaattc gtttccttgc agcgctggcg
    13921 attgcgtgaa cggaggttgc gctgacgcaa ggccgggcgg ttccggaact ggacgagctg
    13981 gaacgtgtca ttgaactggc agggcgaaac gcggaggtcc aggtagcggc ccgtctggtg
    14041 gctgcggaag gactgacgct gcccgtctac acggtggcgc tcggaaaccc gggcgctgcc
    14101 tcggcggccg gcttcttcgg tggcgtgcat gggctcgaac gcatcggctc ggacgtcgtc
    14161 actacctggt tgcacagcct ggtcatgcgg ctgcgctggg acagctcact gcacacgctg
    14221 atggagcgcg tgcgccttgt gttcatgccg atcgtcaatc ccggtggcat gctgcgcggc
    14281 acgcgggcca acctcaatgg ggtggacctc atgcgcaacg cccccgttga tgcgcaggac
    14341 ggcgtgccgt tcctggtagg cggccagcgc atcagcgcag gcctgccttg gtaccggggc
    14401 cgtcgaggcg acgcgatgga gaccgaaagt gcagcgcttt gtgaactggt cgagc
//
LOCUS       DASWIY010000158         4442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2681162, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000158 DASWIY010000000
VERSION     DASWIY010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2681162"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="VGA59_05990"
     CDS             complement(<1..132)
                     /locus_tag="VGA59_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S16"
                     /protein_id="HEX9719264.1"
                     /translation="MIAGRNGNAVTSALTLQSLPLFPLGTVLFPGGVLPLRVFEVRYL
                     "
     gene            158..706
                     /locus_tag="VGA59_05995"
     CDS             158..706
                     /locus_tag="VGA59_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX9719265.1"
                     /translation="MAYQIISFLLDVAIGLLGGACLLRLYMQYQRISFKNPVGRFVFA
                     LTDWLVLPLRRILPAIGRWDTASLAGAFLLELAQFGLLWLLAGRTTGLAVLPLLALFG
                     LLRLVISALTVLVIVYAILSWVRADSPIVDVIDRLCAPLLRPWRRIIPLVGGIDLSPL
                     AFLVALQVAAIVLTSLQSAALR"
     gene            complement(798..1670)
                     /gene="accD"
                     /locus_tag="VGA59_06000"
     CDS             complement(798..1670)
                     /gene="accD"
                     /locus_tag="VGA59_06000"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX9719266.1"
                     /translation="MSWLEKLLPPKIQPTDPAGRRQVPEGLWVKCPSCETVLYKTDLE
                     QNQNVCPTCSHHHRIGARARLNAFLDPEGRYEIGQEVLPVDALKFKDSRKYPERLKEA
                     LENTGETDALVVMGGAVHSINLVAAAFEFDFMGGSMGSVVGERFVRGVESAVEQKVPF
                     VCFTATGGARMQEGLLSLMQMAKTNASLTRLAKKSLPYVSVLTDPTMGGVSAGFAFVG
                     DIVIAEPKALIGFAGPRVIESTVRVTLPEGFQRAEFLQQKGGVDFICDRRELRKTVAH
                     ALAMLQRQPADAVS"
     gene            complement(1742..2551)
                     /gene="trpA"
                     /locus_tag="VGA59_06005"
     CDS             complement(1742..2551)
                     /gene="trpA"
                     /locus_tag="VGA59_06005"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX9719267.1"
                     /translation="MSRIAATFSDLKAQGRKALIPYVTAGFPFADVTPELMRGMVGAG
                     ADVIELGVPFSDPMADGPVIQKAGEKALALGIGTRQVLEMVRQFRQHDDKTPVVLMGY
                     ANPVERYDLLNGKDAFIRDAAAAGVDGVLVVDYPPEECEDFAVKLKARGLDLIFLLAP
                     TSTDERMQQVARVASGYVYYVSLKGVTGAGHLDTGAVELMLPRIRRHVRIPVGVGFGI
                     RDAATARAIGKVADAVVIGTKIIQLIEGQPREQVVPAASEFLRGIRAALDA"
     gene            complement(2548..3798)
                     /gene="trpB"
                     /locus_tag="VGA59_06010"
     CDS             complement(2548..3798)
                     /gene="trpB"
                     /locus_tag="VGA59_06010"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX9719268.1"
                     /translation="MPSYQQPDPTGHFGIYGGSFVSETLTHAISELRDAYARYQHDPQ
                     FLAEFNYELKHFVGRPSPVYHAARMSREQGGAQIYLKREDLNHTGAHKINNVIGQAML
                     ARRMGKPRVIAETGAGQHGVATATICARYGLECVVYMGSEDVKRQSPNVYRMNLLGAG
                     VVPVESGSRTLKDALNEAMRDWVTNVENTFYIIGTVAGPHPYPMMVRDFQSVIGNECL
                     TQMPEMTGGLQPDVVIACVGGGSNAMGIFYPYIPHEKTRLIGVEAAGEGMDSGRHSAS
                     LQRGSPGVLHGNRTYILQDENGQITETHSVSAGLDYPGVGPEHAWLKDSGRAEYVGIT
                     DQEALEAFHYLCRTEGIIPALESSHAIAYAMKLARTMKPEQNILVNLSGRGDKDIGTV
                     ADLAGVDFYDRPSMRGLRVKGGKA"
     gene            complement(3776..>4442)
                     /locus_tag="VGA59_06015"
     CDS             complement(3776..>4442)
                     /locus_tag="VGA59_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX9719269.1"
                     /translation="AVGFVLYAKSPRFVTPQRAAELARRLPPFVTPVLLFVNESPAVV
                     LAACERVAGAAVQFHGDETPAQCMEATAGGKHPFMRAARIALDQRTPFDLVEFAASYP
                     QAQAILLDAHVEGYGGGGKAFDWSRITVPSVPAGPAQTGVALPHVVLSGGLTPANVTD
                     GIVQVRPRCRTLAVDVSSGVEADGPGHQGIKDPEKIHRFVAAVRAGDRLLAQNQNAFL
                     SAT"
BASE COUNT          685 a         1472 c         1445 g          840 t
ORIGIN      
        1 caggtagcgc acttcgaaaa cgcgcaaagg cagcaccccg cccggaaaca ggacggttcc
       61 cagcggaaac agcggcaggg actggagggt gagggcggag gtcacggcgt tcccgtttct
      121 gcccgctatc atcccacgac ctacttatct gcggcttatg gcctaccaaa tcatttcttt
      181 cctgctggac gtcgcgatcg gcctgctcgg cggtgcctgc ctgctgcggc tgtacatgca
      241 gtaccagcgt atctcgttca agaacccggt gggccgtttc gtgtttgcgc tcactgactg
      301 gctggtgctg ccgctgcgcc gaatcctgcc ggcgatcggt cgctgggata cggccagcct
      361 ggccggagcc ttcctgctcg agctggccca gttcggcctg ctctggctgc tggccgggcg
      421 cacgaccggc ctggcggtgc tgcctttact ggcgctgttc gggctgctgc ggctggtgat
      481 ttccgccctc acggtgctgg tgatcgtcta tgcgatcctg tcctgggtgc gggccgattc
      541 gccgatcgtg gatgtgatcg accgcctgtg cgcgccgctg ctgcggcctt ggcgcaggat
      601 cattccgctg gtcggcggca tcgacctgtc gccactggct ttcctggtgg ccctgcaggt
      661 cgccgcaatc gtcctgacgt cgctgcagtc cgcggcgctg cgctagcttg cgtttttcgc
      721 gggtcagatc acaattcgcg accgcggctg atcggcaaca agagcctcat gcgaattgtg
      781 ctctgacccc gacaaactta agagaccgca tccgccggct gccgctgcag catcgccagc
      841 gcatgcgcca ctgtcttgcg cagctcgcgc cggtcgcaga tgaaatccac gccgcctttt
      901 tgctgcagga attcggcgcg ctggaagccc tcgggcagcg tcacgcgcac cgtggattcg
      961 atcacccgcg gcccggcaaa gccgatcagg gccttgggct cggcgatcac gatatcgccc
     1021 acgaaggcaa aacccgcgct gacaccaccc atggtcgggt cggtcagcac actgacgtag
     1081 ggcagggact tcttggccag gcgcgtcagc gaagcattgg tctttgccat ctgcatcagc
     1141 gacagcagcc cttcctgcat gcgcgcgccg ccggtggcgg tgaagcagac aaagggcact
     1201 ttctgctcga cggccgattc gacgccgcgc acgaaacgct cgcccacgac gctgcccatg
     1261 ctgccgccca tgaaatcgaa ctcgaacgcc gcggccacca ggttgatgct gtggacggcg
     1321 ccgcccatga ccaccagggc atcggtttcg ccggtgtttt ccagggcttc cttgaggcgc
     1381 tcgggatatt tccggctgtc cttgaacttg agggcgtcca ccggcagcac ttcctggccg
     1441 atctcgtagc gcccctccgg atccaggaac gcgttcaggc gagcccgggc gccgatccgg
     1501 tggtgatggc tgcaggtcgg gcagacgttc tggttctgct ccaggtcggt cttgtacagc
     1561 acggtttcgc agctcgggca cttgacccac aggccctcgg gcacctggcg gcgcccggcc
     1621 gggtcggtcg gctggatctt ggggggcagc agtttttcta accagctcat cgtgtctagc
     1681 tccttccagg ggaacatgag cggcagcccg ggctgtccgc tcgatgatcc ggagtctgca
     1741 attatgcgtc cagcgccgcg cggatcccgc gcaggaactc cgaggccgcc ggcacgacct
     1801 gttcgcgcgg ctggccctcg atcagctgga tgattttcgt gccgatcacc accgcgtcgg
     1861 caaccttgcc gatggcccgg gccgtggccg cgtcgcggat gccgaagccg acgccgaccg
     1921 gaatgcgcac atggcgtcgt atgcgcggca acatcagctc caccgcgccg gtgtccaggt
     1981 ggcccgcacc ggtcacgccc ttgagcgaga cgtagtaaac gtagccgctg gccacccgtg
     2041 cgacctgctg catgcgttcg tcggtgctgg tcggcgccag caggaagatc aggtccaggc
     2101 cacgggcctt gagcttgacg gcgaaatcct cgcattcctc gggcggatag tcgacgacca
     2161 ggaccccgtc gacgccggca gcggcggcat cgcggatgaa cgcgtccttg ccgttcagga
     2221 ggtcgtagcg ttccaccgga ttggcgtagc ccatcagcac caccggcgtc ttgtcgtcat
     2281 gctgtcgaaa ctggcggacc atctccagca cctggcgggt tccgatgccc agcgccagcg
     2341 ctttctcgcc ggctttctgg atcaccgggc cgtcggccat gggatctgaa aacggcacgc
     2401 ccagctcgat gacgtcggcc ccggcgccga ccatgccgcg catgagttcc ggtgtcacgt
     2461 cggcaaacgg aaagccggcg gtgacgtagg gaatcagcgc cttgcgtccc tgggccttga
     2521 ggtcggagaa ggtggcggcg atgcggctca tgcttttccg cctttgaccc gcaggccgcg
     2581 catcgacggg cggtcgtaga agtccacacc cgccaggtcg gccactgtgc caatgtcctt
     2641 gtcgccccgg ccggacaggt tgaccaggat gttctgctcc ggcttcatgg tgcgcgccag
     2701 cttcatggca tacgcaatcg catggctcga ctccagcgcg gggatgatgc cctcggtgcg
     2761 gcacaggtag tggaaggctt ccagcgcttc ctggtcggtg atgcctacgt actcggcgcg
     2821 gccgctgtcc ttcagccacg catgctccgg ccccacgccg ggatagtcca ggccggcgct
     2881 gacgctgtgc gtttcggtga tctggccgtt ctcgtcctgc aggatgtagg tgcggttgcc
     2941 gtgcagcacg cccggagagc cacgctgcag cgaggccgaa tgcctgccgc tgtccatgcc
     3001 ttcgccggcc gcttccaccc cgatcagccg ggttttctcg tgcgggatgt aaggatagaa
     3061 aatacccatg gcgttgctgc cgccgcccac gcaggcgatc accacgtccg gctggaggcc
     3121 gcccgtcatt tccggcatct gcgtgaggca ttcgttgccg atcacgctct ggaagtcgcg
     3181 caccatcatc gggtaggggt gcggaccggc cacggtgccg atgatgtaga aggtgttctc
     3241 gacgttggtg acccagtcgc gcatggcctc gttgagcgcg tccttcagcg tcctgctgcc
     3301 cgattcgacc gggaccacgc cggcgcccag caggttcatc cgatagacat tggggctctg
     3361 gcgcttcacg tcctcgctgc ccatgtaaac cacgcattcg agcccgtagc gcgcgcagat
     3421 ggttgccgtg gccaccccgt gctggccggc gccggtttcc gcgatcaccc gcggctttcc
     3481 catgcggcgc gccagcatgg cctggccgat cacgttgttg atcttgtgcg cgccggtgtg
     3541 gttgaggtct tcgcgcttca ggtagatctg agcgccaccc tgctcgcggc tcatgcgggc
     3601 ggcgtgatag acgggcgagg gacggccgac gaaatgcttc agttcgtagt tgaattccgc
     3661 caggaattgc gggtcgtgct ggtacctggc gtaggcatcg cgcagttcgc tgatcgcgtg
     3721 ggtcagcgtc tcgctgacga agctgccgcc gtagattccg aaatgacccg tggggtcagg
     3781 ttgctgatag gaaggcattt tgattctggg caagaagcct gtcgccggcg cgcacggcgg
     3841 cgacaaaccg atggatcttt tccgggtcct tgatgccctg atgcccaggg ccatcggcct
     3901 cgacgccgga gctcacatcg acggccagcg ttctacagcg cggccgcacc tgcacgatgc
     3961 catcggtcac gtttgcaggc gtgagtccac cagacaaaac gacgtgaggc agcgcgacgc
     4021 cggtctgtgc cggccctgcc gggacgctgg ggactgtgat acgtgaccaa tcgaaggctt
     4081 tcccgccgcc gccatatcct tcgacatgcg cgtcgagcag gatggcctgg gcctgtggat
     4141 aggaagccgc gaattctacc aagtcgaacg gcgtgcgttg atcgagcgcg attcgcgccg
     4201 cccgcatgaa gggatgcttg ccgcccgcgg tggcttccat gcattgtgcc ggggtctcgt
     4261 cgccgtggaa ctggacagcc gctccggcga cccgctcaca agcggcaagc acgaccgcgg
     4321 ggctttcatt cacgaacagc agcacgggcg tgacgaaggg cggcaatctg cgcgccagct
     4381 ccgccgcccg ctgcggcgtg acgaagcgcg ggctcttcgc atacagcacg aagcccaccg
     4441 cg
//
LOCUS       DASWIY010000159        13897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3057608, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000159 DASWIY010000000
VERSION     DASWIY010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 13897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13897
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3057608"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..809)
                     /locus_tag="VGA59_06020"
     CDS             complement(54..809)
                     /locus_tag="VGA59_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX9719270.1"
                     /translation="MEHASLKQVVSAEEWQLRVDLAACYRLVALYGWSDLVFTHISAR
                     VPGPEHHFLINPYGLMFDEITASSLVKVDGQCNKILDSPFPVNPAGFVIHSAVHEARS
                     DVQCVLHTHTRAGVAVSAQKCGILPISQQSTFVLASLAYHDYEGVAFREEEKPRLQAD
                     LGNANFLVLRNHGLLVTGQTIADAFLQMYTFENTCRIQLDAQAGGELVQVDPRIVSGV
                     AEALRVQTGGLGGAFVWPSLIRKLDRLDPSYKD"
     gene            complement(890..1468)
                     /locus_tag="VGA59_06025"
     CDS             complement(890..1468)
                     /locus_tag="VGA59_06025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX9719271.1"
                     /translation="MKLLVFAGSTRQNSWNRKLAKVVATMASASGAEVTHLELADFDV
                     PMYNADLEARGMPADVTRLKQIAYEHPAWIICTPEYNASYPALLKNTLDWITRPVKSD
                     PVWNDEFRSSRGKVVGVLSASPGALGGLRSQSHLVPLLLNLKCWVAPTNFALGRAADA
                     FDAQGQLVAEPSKKKVQDVIDQVLWAASRLTA"
     gene            1607..3583
                     /gene="mnmG"
                     /locus_tag="VGA59_06030"
     CDS             1607..3583
                     /gene="mnmG"
                     /locus_tag="VGA59_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX9719272.1"
                     /translation="MFYPEEFDVIVVGGGHAGTEAALASARMGCKTLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMAIATDEGGIQFRILNSSKGPAVRATRAQADRI
                     LYKAAIRRRLENQPHLSLFQQAVDDLMVEGDRVVGAVTQVGIRFRSRTVVLTAGTFLD
                     GRIHVGLDNYQAGRAGDPPAISLAARLKELKLPQGRLKTGTPPRLDGRTIDFSKCEEQ
                     PGDGVDRDGSTLASIPVFSFMGTAAMHPRQMPCWITHTNERTHEIIRSGFDRSPMFTG
                     KIEGVGPRYCPSVEDKINRFADKESHQIFLEPEGLTTNEVYPNGISTSLPFDIQYRLV
                     RTMAGLENAHILRPGYAIEYDYFDPRALKSSFETRSIGGLFFAGQINGTTGYEEAAAQ
                     GLFAGVNAALQCQGRQAWLPRRDEAYLGVLVDDLITKGVTEPYRMFTSRAEFRLQLRE
                     DNADMRLTEVGRSMGLVDDARWDAFNRKRDAVSRETQRLKALWVSPRNLPAAESERVL
                     GKAIDHEFNLGDLLRRPDVSYAALMSLDGAAYANPEVSRETLGDLFEPVVEQIEIAAK
                     YSGYIDRQKDEVERAAYFENLTLPPDLDYMQVTALSIEVRQKLNQHRPETLGLASRIS
                     GVTPAAISLLLVHLKKTRMKGAKDAANQVGQAAA"
     gene            3600..4253
                     /gene="rsmG"
                     /locus_tag="VGA59_06035"
     CDS             3600..4253
                     /gene="rsmG"
                     /locus_tag="VGA59_06035"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX9719273.1"
                     /translation="MRDRLQAGLRQGLAGLGLELHETRVAQLLDYLGLIEKWSRVYNL
                     TAVRGPAEMLTHHLLDSLAVVHPLRRQIQERPTRLLDVGSGAGLPGVVIAICCPQVRV
                     DCVDTVAKKSAFIRQVAAVLKLPNLQGLHGRVEQLAGPYEVVTCRAFASLADFVAWSS
                     GALAEGGVWLAMKGKLPQEEIAALPSGVEVFHVEQLEVPGLHAERCLVWMKRRARVT"
     gene            4293..4907
                     /locus_tag="VGA59_06040"
     CDS             4293..4907
                     /locus_tag="VGA59_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX9719274.1"
                     /translation="MFGVADYGAFVAAIIIFLAIPGPGNLALITSTGKGGIAGGLAAT
                     FGVIAGDQVLMWAAVAGVAALLTAYPAAFSAVQWLGAAYLAWLGLKMLLAKPGAKPIL
                     NIEPHHYFKQAGLITLLNPKAIVFYMAFFPLFVDPARHQGLLTFGVMAATIAVLTFAY
                     GLIVVLLTHHLAERMRANPVIGRVLEKVAGMFLLGFGIKLAISK"
     gene            4907..5677
                     /locus_tag="VGA59_06045"
     CDS             4907..5677
                     /locus_tag="VGA59_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9719275.1"
                     /translation="MAKIFCVANQKGGVGKTTTTVNLAAGLAKVGQRVLMVDLDPQGN
                     ATMGSGIDKRKLEMTIYDVLLESASVAEARVRSEKCGYDVLGANRDLAGAEVELVDLP
                     RRDQRLKQALAKVTPDYDFILIDCPPSLSMLTLNGLCSAHGVIVPMQCEYFALEGLTD
                     LVNTIKQVHANMNKDLQIIGLLRVMFDPRITLQQQVSEQLKSHFGEKVFDTVIPRNVR
                     LAEAPSYGLPGVIFDAAARGSLAFVEFAREMVERTQKM"
     gene            5735..6274
                     /locus_tag="VGA59_06050"
     CDS             5735..6274
                     /locus_tag="VGA59_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9719276.1"
                     /translation="MKPSNVLILPGWENSGPQHWQTLWEKRHGYVRVEQHDWTTPLRG
                     DWLARLEEMVLSRDEPAVLVAHSLGCVLAASWSALSNNTHRVKAALLVAPGDVERPEV
                     RELIPTWSPIELRALAFPSVLLASRNDPYCEFERARLFAYAWGAQFMDYGPCGHINAE
                     SGLASWPEGHVLLQDLMKD"
     gene            6325..7311
                     /locus_tag="VGA59_06055"
     CDS             6325..7311
                     /locus_tag="VGA59_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX9719277.1"
                     /translation="MATKKPKGLGRGLEALLGPTAQERGAAGSAATSPGLPASLLLED
                     MVPGSYQPRTRMDEGALYELAESIKAQGIMQPILVRRLSDERAQLRRDREAQGSPNGP
                     AGAYGAERPLYEIIAGERRYRAARLAGLDSVPVLVRDVPDESAAAMALIENIQREDLN
                     PLEEAHGLQRLVKEFGLTHEEAAQAVGRSRSAASNLLRLLNLTEPVQTMLMAGDIEMG
                     HARALLALERAAQITAANQIAAKKLSVREAERLVKKLGAEFNLTSPKPKKEKSRDLRR
                     VEEELSDLLTAAVEVRVKRRVKRNGRSEEMGEVAIQFGSLEELNGLIDKLRG"
     gene            7368..8681
                     /locus_tag="VGA59_06060"
     CDS             7368..8681
                     /locus_tag="VGA59_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719278.1"
                     /translation="MKNRPLNPGAALLALGCLVLSDSAPAQNVKPPKVQLWMDVSTGT
                     MAGMPEMDSMPGMSGMMGGLMGARGPGGGDSNTTYGMARSMNIMPPRVLDIALHNTRK
                     PGVEAAQAIPAGMRMGDSLPLMPPKAQVREREPGEMPQEYKPQTPKGRILIYWGCGQQ
                     VRAGQPRVIDLARGNPAEFGTALAGRYAPDRGARVSPAYALYPNERNTVTLARDSSLV
                     GEHKVLGDGIPASMKFSLGQAQDVMPAIELESRGAVSDSIAASWRPVTNARAYFLNAM
                     GQVGEDMVLWSSSETGDTGMGMFDYLPNNTIDRWLKERVLLQPDVTQCAVPKGIFAGP
                     GGSAGNGGMLRMMAYGGESNFAYPPRPSDPKAVWEPEWAVRVRVKSHTMAMLGEEGAR
                     SAGGRRGQDSRQEMRQEMPGTPAQEAESALPIPNPAGILKGLFGR"
     gene            complement(8736..10160)
                     /locus_tag="VGA59_06065"
     CDS             complement(8736..10160)
                     /locus_tag="VGA59_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9719279.1"
                     /translation="MNAPSELSHLVPEINQRAVPQALIDALKARFGAQCSTALAVREQ
                     HGRDESSFQAPPPAAVVFAERTQDVADAVKLAGDHDVPVIPFGVGTSLEGHLLAVQGG
                     ISIDVSRMNKVLAVNAEDLTVTVQPGVTRKQLNDEIKSTGLFFPIDPGADATLGGMAA
                     TRASGTNAVRYGTMRENVLSLEVVTASGEVIHTGTRAKKSAAGYDLTRLMVGSEGTLG
                     VITEVTVRLYPLPEAISAAVCSFPSIEAAVRTTIQIIQLGVPIARVELIDSNTVRMVN
                     IHSKLQLREEPMLLMEFHGSPSSVKEQAETVQEIAAEHGGNSFEWATTPEERTRLWTA
                     RHNAYFAAIQSKPGCRAISTDTCVPISKLADCLLDSVKEADDSGLPYFLVGHVGDGNF
                     HFGYLLDPDKPEEREIAERLNHQLVARALSLEGTCTGEHGVGLHKMAFLVTETGAGAV
                     DMMRTIKRALDPKNIMNPGKIFSM"
     gene            complement(10252..10587)
                     /locus_tag="VGA59_06070"
     CDS             complement(10252..10587)
                     /locus_tag="VGA59_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX9719280.1"
                     /translation="MKTYASLLAATAGLLVAALAGAQVVPAPAAPVMPGYLPVVPAAP
                     VVRPLPASQWTALHIRQSFELADSDSNGQLTRAEAQQLTILPRSFEEMDENKDGFVAR
                     AEYESVFAP"
     gene            complement(10612..11070)
                     /locus_tag="VGA59_06075"
     CDS             complement(10612..11070)
                     /locus_tag="VGA59_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719281.1"
                     /translation="MPQPPAPATTTPGVAGFATPSPSGLASPLPNPAGVTSQFPAGLP
                     SPTVPNLASPGTAAGSPPVDAGTAPQTAVMGASGYGGVPAGRASPGVGSGPFTALQLA
                     QSFLGADANRDGELTRAEAQRLTIMPYSFEEMDRNHDGILTRFEYEDGVR"
     gene            complement(11407..11577)
                     /locus_tag="VGA59_06080"
     CDS             complement(11407..11577)
                     /locus_tag="VGA59_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719282.1"
                     /translation="MSEQLAQSFKHADLDSDGQLSRQETSGWPAVTRGFDQIDSNKDG
                     SISSAEFDEALK"
     gene            11987..12550
                     /locus_tag="VGA59_06085"
     CDS             11987..12550
                     /locus_tag="VGA59_06085"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX9719283.1"
                     /translation="MGKRLTQIATRTGDDGTTGLGDNTRISKGSLRVHAMGDVDELNS
                     QIGLLLCEQLPGDVTELLVEIQHQLFNLGGELSIPGFELLKPEAVLALDEALAHYNEQ
                     LPKLQEFILPAGNRAASQAHVCRTVARRAERAVVALGNAEELRDTPRQYLNRLSDLVF
                     VLARVLNRMNGGDDVYWKSERLKRSQE"
     gene            complement(12557..13864)
                     /locus_tag="VGA59_06090"
     CDS             complement(12557..13864)
                     /locus_tag="VGA59_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEX9719284.1"
                     /translation="MGMFEWTEKSSEVLRQGGVIAPDERLPWPQSAAMGVQHVIAMFG
                     ATVLAPILMGFDPNIAILMSGIGTLIFFLVTGGKVPSYLGSSFAFIGVVIAATAYAGK
                     GPNANIGVALGGIIACGVLYTLIGAAVQAVGTGWIERVMPPVVTGSVVAVIGLNLAGI
                     PIKNMAASNFDSWMQAVTFVCVGMVAVFTRGMVQRLLILVGLVIASIIYAVLANGLGL
                     GKPLDLSGIANAAWVGMPGFAVPVFDAGAMLMIAPVAIILVAENLGHIKAVTAMTGRN
                     LDQYIGRAFIGDGIATVVSGAAGGTGVTTYAENIGVMAATKIYSTAVFVIAALIAIVL
                     GFSPKFGAVIQAIPLPVMGGVSIVVFGLIAAAGAKIWVDNRVDFSQNRNLIVAAITLV
                     LGTGDFTLKFGAFALGGIGTATFGAILLYALLNREPATARQPA"
BASE COUNT         2387 a         4508 c         4771 g         2231 t
ORIGIN      
        1 cggcatccat gacttttagc gggccgagcc cgcactgcgg cgcacacgga cactcagtcc
       61 ttgtagctgg gatcgaggcg gtccagtttt cggatcaggg acggccagac gaacgcgccg
      121 ccgaggccgc cggtctgcac ccgcaaggcc tcggccaccc cgctgacgat tcgcgggtcc
      181 acctgcacca gttcgccgcc ggcttgcgca tcgagctgga tgcgacaggt gttttcgaag
      241 gtgtacatct gcaggaaggc atccgcaatg gtctggccgg tgaccagcag gccgtggttg
      301 cgcagcacga ggaagttcgc gttgcccagg tcggcctgca gccgcggctt ctcctcttcg
      361 cgaaaggcca cgccctcgta gtcgtggtac gcgagcgacg ccagcacgaa agtcgattgc
      421 tgcgagatcg gcaggatgcc gcatttctgc gcgctcaccg ccacgccggc gcgcgtgtgc
      481 gtgtgcagca cgcactgcac gtcgcttcgc gcctcgtgca ccgcgctatg gatgacgaag
      541 ccggccgggt tcaccggaaa cggcgagtcc aggatcttgt tgcattgccc gtccactttc
      601 accagcgacg aggcggtgat ctcgtcgaac atgaggccgt acgggttgat caggaaatgg
      661 tgctcgggcc ccggcacgcg ggcgctgatg tgcgtgaata ccaggtcgct ccaaccgtac
      721 agcgctacca gccggtagca ggcggcgaga tcgacgcgca actgccattc ctcggcactg
      781 acgacttgct tgagcgatgc atgttccatg atgtgctcca ggattggggc gcccggttgg
      841 ccgcaggcgc aacggcagtt tactttgcta gcaattcaat agcagactat caagcagtga
      901 ggcggctggc tgcccagagc acctggtcga tcacgtcctg gaccttcttt ttcgagggct
      961 cggcgaccag ctgcccctgc gcgtcgaacg cgtccgctgc ccggcccagc gcgaaatttg
     1021 tgggagccac ccagcatttc aggttgagca gcagcggcac caggtggctt tgcgaacgca
     1081 ggccgccgag cgcgcccggt gatgcgctga gcacgcccac gaccttgccg cggctggagc
     1141 ggaactcgtc gttccacacg gggtcggact tcaccggtct ggtgatccag tccagcgtgt
     1201 tcttcagcag cgccggatag ctggcgttgt actccggcgt gcagatgatc caggccgggt
     1261 gttcatacgc gatctgcttg agccgcgtga cgtccgccgg catgcccctc gcttccaggt
     1321 cggcgttgta catcggcacg tcgaagtccg ccaattccag gtgcgtgacc tcggcgccgc
     1381 tggcgctggc catggtggcg acgaccttgg cgagcttgcg gttccaggag ttctggcggg
     1441 tactgccggc gaagacgagg agcttcatgg accgattgca acacaggcgg caggggcgag
     1501 tcgtcggtgc ggtcaagtgg gcgacggcgg cgggaaagtg accgcgccgg taaaattatg
     1561 actttttggg gctcccaccc ccgacgcgcc caggcgcgaa actcccatgt tctaccctga
     1621 agaattcgac gtgatcgtgg tcggcggcgg ccatgcgggc accgaggcgg ccctggcttc
     1681 ggcacgcatg ggctgcaaga cgctgctgct gacccacaac atcgagacac tcgggcagat
     1741 gagctgcaat ccgtcgatcg gcggaatcgg caagggccac ctggtcaagg aggtggatgc
     1801 gctgggcggc gccatggcga tcgccaccga cgagggcggc atccagttcc gtatcctcaa
     1861 ttccagcaag ggaccggcgg tgcgcgccac ccgcgcccag gccgaccgca tcctgtacaa
     1921 ggcggcaatc cggcgccggt tggaaaacca gcctcacctc tcgctgttcc agcaagcggt
     1981 ggacgacctg atggtggagg gcgaccgtgt cgtcggcgcc gtgacacagg tgggcatccg
     2041 cttccgttcg cgcaccgtgg tgctgacggc gggtaccttc ctggacggcc ggatccacgt
     2101 cggcctcgac aattaccagg ccggccgggc cggggacccg ccggcgatct cgctcgcggc
     2161 gcggctgaag gagttgaagc tgccgcaagg ccgcctgaag accggcacgc cgccacggct
     2221 cgacggccgg accatcgatt tttcgaaatg cgaagagcag cccggcgacg gtgtggaccg
     2281 ggacggctcg acgctcgcgt cgattccggt cttcagcttc atggggacgg ccgccatgca
     2341 cccgcgacag atgccgtgct ggatcaccca caccaacgag cgcacgcacg agatcatccg
     2401 ctccgggttc gatcgcagcc cgatgttcac cggcaagatc gaaggggtgg ggccgcgcta
     2461 ttgcccgagc gtggaggaca agatcaaccg cttcgccgac aaggagagcc accagatctt
     2521 tctggagccc gaggggctga ccaccaacga ggtctacccg aacggcatct ccaccagcct
     2581 gccgttcgac atccagtacc ggttggtgcg gacgatggcg ggcctggaga acgcccacat
     2641 cctgcggccc ggttatgcga tcgagtacga ctacttcgac ccgcgggcac tgaaaagcag
     2701 cttcgagacc cgctccatcg gcgggctgtt cttcgccggc cagatcaacg ggacgaccgg
     2761 ctacgaagag gcggcggcgc agggcctgtt cgcgggggtg aacgccgcgc tgcaatgcca
     2821 gggcaggcaa gcgtggttgc cgcgccgcga tgaagcctac ctgggcgtgc tggtggacga
     2881 cctgatcacc aagggcgtga ccgagcctta ccgcatgttc accagccggg ccgagttccg
     2941 gctgcagctg cgcgaagaca acgccgacat gcgactgacg gaagtggggc ggagcatggg
     3001 cctggtggac gatgcccgct gggacgcgtt caaccgcaag cgcgacgctg tttcacgtga
     3061 aacacagcgg ctcaaggcgc tttgggtcag cccgcgcaac ctgccggcgg ccgaatccga
     3121 gcgggtgctc ggcaaggcca tcgaccatga gttcaacctg ggcgatctgt tgcgccggcc
     3181 ggacgtgagc tatgcggcgc tgatgtccct ggacggtgcg gcctacgcca accctgaggt
     3241 ttcacgtgaa acactgggcg atctgttcga gccggtggtc gaacagatcg agatcgccgc
     3301 gaagtattcg ggctacatcg accgccagaa agacgaggtc gagcgcgccg cctatttcga
     3361 gaacctcacg ctaccgcccg acctggacta catgcaggtg acggcgctgt cgatcgaggt
     3421 gcggcaaaag ctcaaccagc accgccctga aaccctgggg ctggcctcgc gcatttcggg
     3481 cgtgacgccg gcggccatct ccttgctcct ggtgcacctg aagaagacgc gcatgaaggg
     3541 agccaaggac gcggccaacc aggtcggcca agccgccgca tgagcgcgaa agccgcagga
     3601 tgagagatcg gcttcaggcc gggctgcgcc agggcctggc tgggctgggg ctggagcttc
     3661 atgaaacccg ggtggcgcaa ctgctggact acctcggcct gatcgaaaag tggagccggg
     3721 tctacaacct gacggcggta cgcggcccgg ccgaaatgct gacccatcac ctgctcgaca
     3781 gcctggcggt ggtgcacccc ttgcgccgcc agatccagga gcggccgacc cgcttgctcg
     3841 atgttgggtc gggcgccggc ctgccgggtg tcgtcatcgc gatctgttgc cctcaggtcc
     3901 gggtcgactg cgtcgatacg gtcgccaaga aatccgcctt catccggcag gtggcggcgg
     3961 tgctgaagct gccgaacctg caaggcttgc acgggcgggt agagcaactc gcgggcccct
     4021 atgaggtggt gacctgccgg gccttcgcgt cgctggccga tttcgtggcc tggtcttcag
     4081 gcgcgctggc cgagggcggc gtctggctgg ccatgaaggg caagctgccg caggaggaaa
     4141 tcgccgcgct gccgtccggg gtggaggtgt ttcacgtgga acagctggag gtacccggcc
     4201 ttcacgccga acgctgtctc gtctggatga aacggcgcgc gcgcgtaacg taaactcggc
     4261 tttcagttcg gcatcgagca caggggattt ccatgtttgg cgtagcagac tacggcgcgt
     4321 tcgtcgcggc gatcatcatt ttcctggcca tccccggtcc cggtaacctg gcactcatca
     4381 cttcgaccgg caagggcggc atcgcgggcg gcttggccgc caccttcggg gtgatcgccg
     4441 gcgaccaggt gctgatgtgg gcggcggtgg ccggcgtggc cgcgctgctg acggcttatc
     4501 cggccgcctt ctccgccgtc caatggctgg gcgccgccta cctggcatgg ctggggctga
     4561 agatgctgct ggccaagccc ggcgccaagc ccatcctcaa catcgagccg caccactatt
     4621 tcaagcaggc cggcctgatc accttgctca accccaaggc catcgtcttc tatatggcgt
     4681 ttttcccgct gttcgtggac ccggcgcggc accagggctt gctgaccttc ggcgtgatgg
     4741 cggccaccat cgccgtgctg accttcgcct atggcttgat cgtcgtgctg ctgacccatc
     4801 atctggctga gcggatgcgt gccaatccgg tcatcggccg ggtgctggaa aaggtggccg
     4861 gcatgttcct gctcggcttc ggcatcaagc tggcgatctc gaaataatgg ccaagatctt
     4921 ttgcgtcgcc aaccagaagg gcggggttgg caagaccacc acgacggtca atcttgctgc
     4981 cgggctggcg aaggtcgggc agcgggtgct gatggtcgac ctggacccgc agggcaatgc
     5041 gaccatgggc tccggcatcg acaagcgcaa gctagaaatg acgatctacg acgtgctgct
     5101 ggagtcggct tcggtggccg aagcgcgggt caggagcgag aagtgcggct acgacgtgct
     5161 gggcgccaac cgtgacctgg ccggggccga ggtggaactg gtggacctgc cacgtcgcga
     5221 ccagcgtctc aaacaggccc tggccaaggt cacccccgac tacgacttca tcctgatcga
     5281 ctgcccgccc tcgctgtcca tgctcacgct caatggcctg tgctcggcgc acggcgtgat
     5341 cgtgccgatg cagtgcgaat atttcgcgct ggagggactc acggacctgg tcaacaccat
     5401 caagcaggtc cacgccaaca tgaacaagga cctgcagatc atcgggctgc tgcgcgtgat
     5461 gttcgatccg cgcatcaccc tgcagcagca ggtgagcgag cagctgaagt cgcatttcgg
     5521 cgagaaggta ttcgacacgg tgatcccgcg caacgtccgc ctggccgagg cgcccagtta
     5581 cgggctgccc ggcgtgatct tcgacgcggc cgcgcgcggc agcctggcct ttgtcgaatt
     5641 cgcgcgcgag atggtggagc gcacccagaa gatgtaagcg aaatctgcgg catgccgcat
     5701 gaaggctaga ttccaggcta tcgatttcat agcaatgaaa ccttccaacg tcctcatcct
     5761 gccgggctgg gaaaacagcg ggccgcagca ttggcaaacc ctgtgggaaa agcgccatgg
     5821 ctacgtgcgc gtcgagcagc atgactggac gacgccgctg cgcggcgact ggctggcgcg
     5881 gctggaggag atggtgctgt cgcgcgacga gccggcggtg ctggtggcgc acagcctggg
     5941 ctgcgtcctc gccgcctcct ggtcggcgct gtcgaacaac acccaccgcg tcaaggcggc
     6001 gctgctggtc gcgcccggcg atgtggagcg gcccgaggta cgggagctga tccccacgtg
     6061 gtcgccgatc gagctgcgcg cgctcgcctt tccgagcgtg ctgctggcca gccgcaacga
     6121 cccctattgc gagttcgagc gcgcgcgcct gttcgcctac gcgtggggcg cgcaattcat
     6181 ggactacggc ccgtgcggcc atatcaacgc ggagtccggc ctggccagtt ggcccgaggg
     6241 ccatgtgctg ctgcaggacc tgatgaaaga ctgatgaccg atgacgaacg aatcccaagc
     6301 tcatactcca aggcgggagg cctgatggcg accaagaaac ccaaggggct gggtcgcggc
     6361 ctggaagcgc tgctgggccc cacggcacag gagcgcgggg ccgccggcag cgcggccacc
     6421 agccccggcc tgccggcttc cctgctgctg gaagacatgg tgcccggcag ctaccagccg
     6481 cgcacgcgca tggacgaagg cgccctgtac gaactggccg agagcatcaa ggcgcaaggc
     6541 atcatgcagc cgatcctggt gcggcgcctg agcgacgaga gagcgcagct gcgccgtgac
     6601 cgcgaagcgc aagggtcacc caatgggccg gctggtgcgt acggagctga acggcctctg
     6661 tacgagatca tcgcgggcga gcgacgctat cgcgccgccc ggcttgccgg cctggacagc
     6721 gtgccggtgc tggtgcgcga cgtgccggac gaatcggcgg cggcgatggc actgatcgag
     6781 aacatccagc gcgaagacct gaatccgctg gaagaagcgc acggtctgca gcgcctggtc
     6841 aaggaattcg gtctcaccca cgaagaggcg gcccaggccg tgggccgctc ccgcagcgcc
     6901 gccagcaacc tgttgcgcct gctcaatctg accgagccgg ttcagaccat gctgatggcc
     6961 ggcgacatcg aaatgggcca tgcccgcgcg ctgctcgcgc tggagcgcgc ggcacagatc
     7021 acggcggcca accagatcgc cgcgaaaaag ctgtcggtgc gcgaggcgga gcgcctggtc
     7081 aagaaactgg gcgccgagtt caacctcacg tcgcccaagc ccaagaagga aaaatcgcgc
     7141 gacctgcgac gcgtggaaga ggaactctcc gacctgctca ccgccgcagt cgaggtgcgc
     7201 gtgaaaaggc gcgtcaagcg caacggccgc agcgaggaaa tgggcgaagt ggcaatccag
     7261 ttcggatcgc tggaagagtt gaacggcctg atcgacaaat tgcgcggctg agccacgggc
     7321 ctatactcgc ttgcctggca ggcgccaggc atcaggaggg cctcaagatg aagaatcgtc
     7381 cgctcaaccc gggtgccgca ttgctggcat tgggttgcct tgtcctgtcc gacagcgcgc
     7441 cggcgcagaa cgtgaaaccg cccaaggtcc agctctggat ggacgtgtcc accggcacca
     7501 tggcgggcat gccggagatg gactccatgc cgggcatgag cgggatgatg ggcggcctga
     7561 tgggcgcgcg cggtcctggc ggcggtgaca gcaacaccac ctacggcatg gcgcgttcga
     7621 tgaacatcat gccgccgcgc gtgctggaca tcgcgctgca caacacgcgc aaacccggcg
     7681 tcgaagccgc gcaggccatt ccggccggca tgcggatggg cgacagcctg ccgctgatgc
     7741 cgccgaaggc acaggtgcgc gagcgcgagc cgggcgagat gccgcaggag tacaagccgc
     7801 agacgccgaa gggccgcatc ctgatctact ggggctgcgg ccagcaggtg cgcgcgggcc
     7861 agccccgcgt gatcgacctg gcgcgcggca atccggccga gttcggcacg gcgctggcgg
     7921 ggcgttacgc gcccgatcgc ggcgcgcggg tgagcccggc ttacgcgctc tatccgaacg
     7981 agaggaacac cgtgacactg gcgcgcgaca gctcgctggt gggcgaacac aaggtgctcg
     8041 gcgacggcat cccggcctcg atgaagttct ctctgggcca ggcccaggac gtcatgccgg
     8101 ccatcgagct ggaaagccgc ggagccgtga gcgacagcat cgcagccagc tggcggccgg
     8161 tgacgaatgc acgcgcttac ttccttaatg cgatgggcca ggtcggcgag gacatggtgc
     8221 tgtggtcgag ctccgagacg ggcgacaccg gcatgggcat gttcgactat ctgccgaaca
     8281 acaccatcga ccgttggctc aaggagcgcg tcctgcttca gccggacgtg acgcagtgcg
     8341 ccgtgcccaa gggcatcttc gccgggccgg gcggcagtgc aggcaatgga ggcatgctgc
     8401 gcatgatggc ctatggcggc gagagcaact ttgcctaccc gccgcggccg tccgatccca
     8461 aggcggtgtg ggaacccgag tgggccgtgc gggtgcgcgt gaagtcgcac acgatggcca
     8521 tgctgggcga agaaggcgcg cgttcggcgg gcggtcggcg cggtcaggat tcacgacagg
     8581 aaatgcggca ggaaatgccc ggcacgccgg cccaggaggc cgagtcggca ctgcccatcc
     8641 ccaacccggc gggcatcctc aaaggcctgt tcggccggta ggcgcgaggc ctgccgggtt
     8701 tgacccggac caacctcgtc tgccgcgcgg gacacctaca tcgaaaagat cttccccggg
     8761 ttcatgatgt tcttggggtc cagcgcccgc ttgatcgtgc gcatcatgtc cacggcgccg
     8821 gcgccggttt cggtgaccag gaacgccatc ttgtgcaggc ccacgccgtg ctcgccggtg
     8881 caggtgccct ccaggctgag ggcgcgtgcc accagctggt gattgagtct ctctgcgatc
     8941 tcgcgttcct cgggcttgtc ggggtccagc aggtagccga aatggaaatt gccgtcgccg
     9001 acatggccca ccaggaagta gggcaggccg ctgtcgtcgg cttccttgac cgagtccagc
     9061 aggcagtcgg ccagcttcga gatcggcacg caggtgtcgg tggaaatcgc gcggcagccg
     9121 ggcttggact ggatcgcggc gaaataggcg ttgtgccggg cggtccaaag gcgcgtgcgc
     9181 tcctccggcg tggtagccca ctcgaaggag ttgccgccat gctcggccgc gatctcctgc
     9241 accgtctcgg cctgctcctt cacgctcgag ggcgagccgt ggaactccat cagcagcatc
     9301 ggctcttcgc gcagttgcag cttgctgtga atgttgacca tgcgcacggt gttgctgtct
     9361 atcagttcga cccgcgcgat cggcacgccc agctggatga tctggatggt ggtgcgcacc
     9421 gcggcctcga tgctcgggaa cgagcagacc gccgcagaga tcgcctcggg cagcggatac
     9481 aggcgcaccg tcacttcggt gatcacgccc agcgtgcctt cgctgccgac catcaggcgc
     9541 gtcaggtcgt agccggcggc cgactttttg gcgcgggtgc cggtgtggat gacctcgccg
     9601 ctggccgtca ccacctccag gctgagcacg ttctcgcgca tggtgccgta gcgtacggcg
     9661 ttggtgccgc tggcgcgcgt ggcggccatc ccgcccaggg tcgcgtcggc gccgggatcg
     9721 atcgggaaga acaggccggt gctcttgatt tcatcgttga gctgcttgcg cgtgacgccc
     9781 ggctgcaccg tcaccgtcag gtcctcggcg ttgacggcca gcaccttgtt catgcggctg
     9841 acgtcgatgc tgatgccgcc ctggacggcc agcaggtggc cctcgagcga ggtgccgaca
     9901 ccgaagggga tgacgggcac gtcgtggtcg cccgccagct tcacggcgtc ggccacgtcc
     9961 tgcgtgcgct cggcgaatac cacggcggcc gggggcggcg cctggaacga ggactcatcc
    10021 cggccatgct gttcgcgcac cgcgagtgcc gtggagcact gggcgccgaa acgcgccttg
    10081 agggcatcga tcagcgcctg cggcacggca cgctgattga tttcggggac gaggtgcgac
    10141 agttccgagg gtgcgttcat gctggactcc tgggaccgag tttaaggctt gcgcttgccc
    10201 cgcgtgctgc cgcgagccta tgcccagccg ccgcgcatga aggcattcgg cctacggcgc
    10261 gaacacgctt tcgtattcgg cgcgggccac gaacccgtcc ttgttctcgt ccatctcctc
    10321 gaagctgcgc ggcaggatcg tcaactgctg ggcttcggcg cgggtgagct ggccattgct
    10381 gtcgctgtcg gcgagctcga acgactgccg gatgtgcaac gcggtccatt ggctggccgg
    10441 cagcggccgc acgaccggcg cggcgggaac gaccggcaag taacccggca tgacgggcgc
    10501 ggccggcgcc ggcaccacct gggcgcccgc gagcgcggcg accagcaagc cagccgtggc
    10561 agcaagcagg gatgcgtagg ttttcatggt gcttggcctt tccgctgtgg ctcagcgcac
    10621 gccgtcctcg tattcgaagc gcgtgaggat accgtcgtgg ttgcggtcca tctcctcgaa
    10681 gctgtacggc atgatcgtca gccgctgggc ttccgcccgg gtgagctccc catcccggtt
    10741 ggcatcggcg cccaggaagg attgcgcgag ctgcagcgcg gtaaaaggac cggaccccac
    10801 gcccggcgag gcgcgcccgg caggaacacc accgtaaccc gatgcgccca tcaccgcggt
    10861 ctgcggcgcc gttccggcgt cgaccggcgg gctgccggcc gctgtccccg gcgaagccag
    10921 gttgggcacg gtgggtgaag gcagcccggc cggaaactgc gaggtcacac cggcggggtt
    10981 cggcagcggc gacgccagcc cggacggact cggcgtggcg aacccggcga cgcccggggt
    11041 ggtcgtggcg ggcgcggggg gctgcggcac tgattgtaac gaacggcttg acgtcggtgc
    11101 cggcgcgacg ccaatgggtg cggcgggtga cgggcggccc ggggtcaggg tctggcccac
    11161 tgcggcggcg ccggcgaaca aagccaaggc gcagcccagg cgcagggcgc gtcgcgtagt
    11221 ggagagggcg ttcacagcat gactcctgga gagcgcaggc ctggaacaca acaaggccgg
    11281 gcccgccctc gatgccatct ttgcggccgg cggcggctcg gccaatacgg caactgcgcc
    11341 aaacgttgta ggactagtcc actgaggacc aagcgcaccg cgccacccgg tcccgtgggt
    11401 tgccgcctat ttgagcgctt cgtcgaactc ggcgctggag atcgagccgt ccttgttgct
    11461 gtcgatctgg tcgaagccgc gcgtcacggc gggccagccc gaggtttcct ggcggctgag
    11521 ctggccgtcg ctgtcgaggt cggcgtgctt gaaggattgc gccagctgct cggacacctg
    11581 ctggtagggc ggggccgccc tgctgccgtc cgcgggtgtt gggcctgacc gagagccggc
    11641 cattccccgg cgcggagtcg agggcaccgc ggcagtggtg gaccgcaccg tcgagggggt
    11701 ggagctgatg gaagaagggg tggcgggcgc ctgagaccgc gccggaagcg cggccccgcc
    11761 aagcgccagg gaggtaaaca gcgctacgct gcaggattcg aaattgggaa ggcgtcgtcg
    11821 tttcacatgc atcttgaaag tgctcctgaa gttgagggac tccgcattgc agccgatcac
    11881 ggcgcatgag gccagcactt ttgcctcaag ttgcctgcga aacatccggt gcagccacgt
    11941 aacgcacaat ccgtgcaagc actttcacca acgagagagg ttcgacatgg gcaaacgcct
    12001 gacacagatc gccacccgca ccggcgacga tggcaccacc ggcctgggcg acaacacccg
    12061 catctccaag ggcagcctgc gcgtgcatgc catgggagat gtggacgaac tcaattcgca
    12121 gatcggcctg ctgctgtgcg agcagttacc gggggatgtg accgagctgc tggttgaaat
    12181 ccagcaccag ctgttcaacc tgggcggcga gctttccatt ccgggtttcg aactgctcaa
    12241 acccgaggcc gtcctggcgc tggacgaggc gctggcccat tacaacgagc agctgccaaa
    12301 gctgcaggag ttcatccttc cggccggcaa ccgcgccgcg tcgcaggcgc atgtctgccg
    12361 cactgtggcg cgccgggccg agcgcgcggt ggtggcgctg ggcaacgccg aggagctgcg
    12421 cgatacgccg cgccaatacc tcaaccgcct gagcgacctc gtcttcgtgc tggcgcgcgt
    12481 gctcaatcgc atgaacggcg gcgacgacgt gtactggaaa agcgaacggc tgaagcgctc
    12541 gcaggagtag gcgcgctcag gcgggctggc gcgccgtcgc cggctcgcgg ttcagcagcg
    12601 catacagcag gatcgcgccg aaggtcgcgg tgccgatgcc gcccagcgcg aaggcaccga
    12661 acttgagcgt gaagtcgccc gtgcccagca ccagcgtgat cgcggcgacg atcaggttgc
    12721 ggttctgcga gaagtccacc ctgttgtcga cccagatctt ggcaccggcc gccgcgatca
    12781 gcccgaacac cacgatcgac acgccgccca tcaccggcag cggaatggcc tggatgacgg
    12841 cgccgaactt cggcgagaag cccagcacga tggcgatcag cgccgcgatc acgaagacgg
    12901 cggtggagta gatcttggtg gcggccatga caccgatgtt ctcggcatag gtggtcacgc
    12961 cggtgccgcc cgccgcgccg ctgacgaccg tggcaatgcc gtcgccgatg aaggcgcggc
    13021 cgatgtactg gtcgaggttg cggccggtca tcgccgtgac ggccttgata tggccgaggt
    13081 tctccgccac caggatgatc gccaccggcg cgatcatcag catggcgccg gcgtcgaaca
    13141 ccggcaccgc gaatccgggc atgccgaccc acgccgcatt ggcgatgccc gacaggtcga
    13201 gcggcttgcc caggcccagg ccgttcgcga gcacggcata gatgatgctg gcgatcacga
    13261 ggcccaccag gatcagcagg cgctgcacca tgccgcgcgt gaacaccgcg accatgccga
    13321 cgcagacgaa ggtcaccgcc tgcatccatg aatcgaagtt gctggccgcc atgttcttga
    13381 tcgggatacc cgcgaggttc aggccgatca cggccaccac cgagccggtg acgaccggcg
    13441 gcatgacccg ttcgatccag cccgtgccca ccgcctggac ggccgcgccg atcagcgtgt
    13501 acagcacacc gcaggcgatg atgccgccca gcgccacgcc gatgttggcg ttgggaccct
    13561 tgcctgcgta ggctgtcgcc gcgatcacca cgccgatgaa ggcgaagctg gaacccaggt
    13621 agctcggcac cttgccgccg gtgaccagga agaagatgag ggtgccgata ccgctcatca
    13681 ggatggcgat attgggatcg aaccccatga ggatcggcgc gagcacggtc gcgccgaaca
    13741 tcgcaatcac gtgctgcacg cccatcgccg cgctctgcgg ccacggcagg cgctcgtcgg
    13801 gcgcgatcac gccgccttgc cgcagcacct cggaggactt ctcggtccat tcgaacattc
    13861 ccatcgctcg ctccttgttg ttggtgcggc gatgcta
//
LOCUS       DASWIY010000160         4157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2682370, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000160 DASWIY010000000
VERSION     DASWIY010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2682370"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..773)
                     /locus_tag="VGA59_06095"
     CDS             complement(30..773)
                     /locus_tag="VGA59_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9719285.1"
                     /translation="MRFGNTIYRMAAIALIGGLTCGPAAPADADMTDAWTISEGGKLY
                     DAWYKVLSRNMKDTPTHPSYPAAGKQKGAATWRCKECHGWDYKGKDGAYSKGSHFTGI
                     KGIRVLAGVPPERIASVLRDKTHGYTEAMIPNQAVAALALFVSRGQVEFDQFIDRATK
                     KAAGDPKRGARFYQTICATCHGFDGKEINFGKPEDPEFIGTVASENPWETLHKIRNGQ
                     PGVPMPSLGVLELKDQVDIVSYAQTLPKK"
     gene            complement(1042..1302)
                     /locus_tag="VGA59_06100"
     CDS             complement(1042..1302)
                     /locus_tag="VGA59_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719286.1"
                     /translation="MNCHAGLDPQRQHLEHGLAPFPRIGEAAAKQLKHVERLASIVPA
                     DQITRTSEKVAVKQGLQLRVFTSMTEAIRWLLEPPLRPPSLG"
     gene            complement(1308..2033)
                     /locus_tag="VGA59_06105"
     CDS             complement(1308..2033)
                     /locus_tag="VGA59_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9719287.1"
                     /translation="MKAKSAALLAAKRQDAPARPKTAKRRATTRLPAAERRALILEKA
                     FEFFSEYGLTAQTRGLAQACGVSQRLLYSIFPNKAALINAVYDASIAGPFKAIWFVHL
                     RDRGRPLEQRLNEFYQEYYDTILTRRWLRLFLYASLAEVEMAPTYIAAIIRTLLETVV
                     DEAASDAGLPMPSDRALIQELGWTLHGNVSHLAIRRHIYRDATSLDVHRVIALHVTTF
                     LAGVRSVLDAGEAAAKPKSRRVS"
     gene            2172..2483
                     /locus_tag="VGA59_06110"
     CDS             2172..2483
                     /locus_tag="VGA59_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX9719288.1"
                     /translation="MIFEMRTYRVQPGKAGELLKLYQEQGLPIISRYARLIGCWTQES
                     GVLNSVVFIWAYEDFGHRAAQRAKLAQDAQWGGFVPAMLPYLVHQESVFLSPAAFSPI
                     K"
     gene            2729..4057
                     /locus_tag="VGA59_06115"
     CDS             2729..4057
                     /locus_tag="VGA59_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9719289.1"
                     /translation="MNKLWIPASSRRDFLKMSAAAGAASAGLLPLAGNAQAATLNFHT
                     WSAAVDAVKSHLTAFEAKTGIKVNYNNAPWAQYRDTMVTKFVGKAPMDVLWVSDSWLP
                     EWADAGWLAPVNGFPELMKYNEATDDFCVKSMQYKGKQYGLTYYSDYMAFFYDEEMLK
                     KAGFNAPPQTWDEVVQQSAKIKQMGLSQYPLMLSMARESWLVEFLTALVYSNGGRFTD
                     DNGAAVMADPKRGAQRALQWVVDAVNKHKIVSPACVEIGELNGLKSFTSGNHAFAMLG
                     RYRIRTLNDPKQSQIAGRVKQALMPAGPGGSHATVGWMRFYGLTAQAAADKARAANAA
                     KLIEWFGGKADGKYQFQKMLFTDIGSGFGVKELFKDPDVQASYQKYSDLAMFEKQQQL
                     ARKKDVISRWFGEWDEVNGTAWQSAIIGKSTAADALKKSAQAWNDMRKQT"
BASE COUNT          740 a         1289 c         1377 g          751 t
ORIGIN      
        1 ggcggtggct gctcgccttc atcgcggcgt cacttcttcg gcagggtttg ggcgtaggaa
       61 acaatgtcca cctggtcctt caattcgaga accccaagcg acggcatcgg gacgccaggc
      121 tggccgttgc ggatcttgtg cagcgtttcc caagggtttt cgctggcgac ggtgccgatg
      181 aattcgggat cttcgggctt gccgaaattg atctccttgc cgtcgaagcc atggcatgtc
      241 gcgcagatgg tctggtagaa acgcgcgccg cgtttcggat cgccggcggc cttcttggtg
      301 gcgcggtcga tgaattggtc gaattcgacc tggccgcgac tgacgaacag cgccaatgcc
      361 gccaccgcct ggttcggaat catcgcctct gtgtagccgt gggtcttgtc gcgcagaacg
      421 ctcgcgatgc gctccggggg gacgccggcc aagacccgga tgcccttgat gcccgtgaag
      481 tgggagccct ttgaataggc cccgtccttg cccttgtagt cccagccatg gcattccttg
      541 cagcgccacg tggccgcacc tttctgcttg cccgccgcgg gatacgacgg atgagtcgga
      601 gtgtccttca tgttccggga cagcaccttg taccaggcgt cgtagagttt gccgccctcg
      661 gagatcgtcc aggcatccgt catgtcggcg tccgccggcg cggctggacc gcaggtcaag
      721 cccccgatca atgcgatagc cgccattcga tagatcgtgt tgccaaatct catggtcgtt
      781 cctttgtgtg ccccgacgtc ttcggtgcga tcactgtgcg atcgactcgg acgtcgcaca
      841 agggctgatg tgcgcggttt ggccgggacc atagtccctg ctcttgggac cttggtccat
      901 gggcacgccg ggtcacgctc tcgcgaatat gaaagtcttt gcccgatttc cgtgcgtcgc
      961 gcgggaagcg acacagtgat cgcggtggcg gagttctacc ggcgcagcgg cgtggcgcga
     1021 gccgcacgtc cgcttggtgc actagcccaa ggaaggaggc cgcagtgggg gctccaacag
     1081 ccagcgaatc gcctcggtca tcgaagtgaa aacgcgaagc tgcaggcctt gcttcacggc
     1141 gactttctcg ctggtgcgcg tgatctggtc ggcgggaacg atggaggcca gccgctccac
     1201 gtgcttgagc tgcttcgccg ccgcttcccc gatccgcgga aagggagcca gtccatgctc
     1261 aagatgctgt cgttgcgggt caagcccggc atgacaattc accccggtta cgaaacgcgc
     1321 ctggacttcg gtttcgccgc ggcttcgccg gcatcgagta ccgaccgcac gccggccagg
     1381 aaggtggtca cgtgcagcgc gatgacgcgg tgcacgtcga gcgaggtggc gtcgcggtag
     1441 atgtggcggc gaatggccag atgcgacacg ttgccgtgca gcgtccagcc gagctcctgg
     1501 atgagggcgc ggtcggacgg catcggcaac ccggcgtcgc tcgccgcttc gtccaccacc
     1561 gtttccagca gcgtcctgat gatggcggcg atgtaggtcg gcgccatctc cacttcggcc
     1621 agcgacgcgt acaggaacag ccgtagccag cggcgcgtga ggatggtgtc gtagtactcc
     1681 tggtagaact cgttcagccg ctgttccagc ggcctgccgc gatcgcgcag gtgcacgaac
     1741 cagatggctt tgaaggggcc ggcaatgctg gcgtcgtaga ccgcgttgat cagggcggcc
     1801 ttgttcggaa agatgctgta gagcagccgc tgcgagacac cgcaggcctg agcgaggccg
     1861 cgggtctggg ccgtcagccc gtattcggag aagaactcga acgctttttc caggatcagc
     1921 gcccggcgct ccgccgcagg cagccgggtg gtggcacggc gcttggccgt cttgggccgc
     1981 gcgggcgcgt cctggcgctt ggcggcaagc agggctgctg atttggcttt catgggcgca
     2041 atgttgcggc gggcggggcc cgcgatgtga gcggactacg cactttagcg ccaactatat
     2101 cgtcaaatga cgaggcatgg gttaccattt cggcgacaaa attccaggca cgtccaccca
     2161 cgggggaaat catgatcttc gaaatgcgca cctaccgcgt ccagcccggc aaggcagggg
     2221 aactcctcaa gctttaccag gaacagggct tgcccatcat ctcgcgctac gccaggctga
     2281 ttggctgctg gacgcaggag tcgggcgtgc tcaactcggt ggttttcatc tgggcttatg
     2341 aggacttcgg ccaccgggcg gcgcaacgcg ccaagcttgc gcaggatgcg cagtggggtg
     2401 gcttcgtgcc ggcgatgctg ccgtacctgg tgcaccagga aagcgttttt ctcagcccgg
     2461 cggcgttctc tccgatcaag taggtcagcc cggcaatacg ccggcaggtc ccttaagtac
     2521 acggaaagtt ccgcgttcat cgcggtcgtg gccgacgcgc tttgatcggc gtcaagaatt
     2581 ctttaagtcg tcacttgacg atatatatcc ccatcaacaa taatgccatc cggtcgagtc
     2641 ggcagcggtg ggctggcggc ttgcgcaccc gatggcccgg cacggcccct gccaggattt
     2701 gttgccgcac gtacgaggaa agatcgccat gaacaagtta tggatccctg catcgagtcg
     2761 ccgtgatttc ctgaagatgt ccgccgcggc gggcgcggcc agcgcgggac tgctgccgct
     2821 ggcgggcaac gcccaggccg cgacgctgaa cttccacacc tggtcggccg cggtcgacgc
     2881 cgtgaagagc catctcacgg cgttcgaggc gaaaaccggc atcaaggtga actacaacaa
     2941 cgcgccctgg gcccagtacc gcgacaccat ggtcaccaag ttcgtcggca aggcgccgat
     3001 ggacgtgctg tgggtgtccg acagctggct gcccgaatgg gccgacgccg gctggctcgc
     3061 gccggtcaac ggctttcccg agctgatgaa gtacaacgag gcgaccgacg acttctgcgt
     3121 caagtcgatg caatacaagg gcaagcagta cggcctgacc tattactcgg actacatggc
     3181 gttcttctac gacgaggaga tgctgaagaa ggccggcttc aacgcgccgc cccagacctg
     3241 ggacgaagtc gtgcagcagt cagccaagat caaacagatg gggctgtccc agtacccgct
     3301 gatgctgtcc atggcacgcg agagctggct cgtcgaattc ctcaccgcgc tggtctattc
     3361 gaacgggggc cgtttcacgg acgacaacgg cgcggccgtg atggccgatc ccaagcgcgg
     3421 cgcgcagcgg gcgctgcagt gggtggtgga cgccgtcaac aagcacaaga tcgtttcgcc
     3481 cgcctgcgtg gagatcggtg agctcaacgg gctcaagtcg ttcacctccg gcaatcacgc
     3541 cttcgccatg ctgggccgct accgtatccg cacgctgaac gacccgaagc agtcgcagat
     3601 cgcgggccgg gtcaagcagg cgctgatgcc cgccgggccg ggcggctctc acgcgacggt
     3661 gggctggatg cgcttctacg gcctgacggc gcaggcggcc gccgacaagg cgcgcgccgc
     3721 caacgcggcc aaactgatcg agtggttcgg cggcaaggcc gacgggaagt accagttcca
     3781 gaaaatgctg ttcaccgaca tcggctcggg cttcggcgtc aaggaactgt tcaaggaccc
     3841 cgacgtgcag gcctcgtacc agaagtactc cgacctggcc atgttcgaga agcagcagca
     3901 actcgccagg aagaaggacg tcatctcgcg ctggttcggt gagtgggatg aagtcaacgg
     3961 aacggcctgg cagtcggcca tcatcggcaa gagcaccgcg gcggacgcac tcaagaaatc
     4021 ggcccaggca tggaacgata tgcgcaagca gacttgagcg agccggcacc gccgctcgaa
     4081 ccgccgggac tcgtccccgg cggggcggcg ccgcggaccg cagagcgcaa ggcttggggg
     4141 tggcgcgacc tgctcgg
//
LOCUS       DASWIY010000161        17691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2683206, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000161 DASWIY010000000
VERSION     DASWIY010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 17691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17691
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2683206"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VGA59_06120"
     CDS             <1..975
                     /locus_tag="VGA59_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9719290.1"
                     /translation="GQLVVVSRDLGTAHYATGIADRLQQVLDDWSFLSPQLQDLYETL
                     NGGKARHAFPFESERCMAPLPRACQWADGSAYINHVELVRLARHAEVPANYYTEPLMY
                     QGGSDDFLGPQDDVIVPSEEYGIDFEAEVAVVTGDIAMTATPDQALEGIRLVMLANDV
                     SLRNLIPDELAKGFGFFQSKPATAFSPVAVTLDELGDAWDKGRLHLTLQSTWNGRTVG
                     MCDAGKEMTFHFGQLIAHICKTRNVRAGSIIGSGTVSNRATEADGRKEWTKGYSCIAE
                     KRAIETIMDGKPATEYMKFGDTIRIEMKGRDGQSVFGAIDQKIAPLKR"
     gene            1020..1439
                     /locus_tag="VGA59_06125"
     CDS             1020..1439
                     /locus_tag="VGA59_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HEX9719291.1"
                     /translation="MNDPHNLPRFLEAQAPVYDQVLAELADGRKQSHWIWFIFPQLAG
                     LGHSAMARRFAIASLDEAAAYLAHPVLGARLRECSRLVLDLEERSVHDIFGSPDDLKF
                     RSSMTLFDLAGPAEPVFRQCLLKYFKGQGDPQTLALL"
     gene            complement(1479..2897)
                     /gene="otsA"
                     /locus_tag="VGA59_06130"
     CDS             complement(1479..2897)
                     /gene="otsA"
                     /locus_tag="VGA59_06130"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HEX9719292.1"
                     /translation="MSRLVVVSNRVADPRKTAAGGLAVALAEVLSNTGGLWFGWSGKI
                     VEDAAGGKPGEGELHSQQAGPVRLVTLDLSREDHAAYYVGYSNGVLWPVFHYRLDLAD
                     FDAGYIAGYRRVNQLFARKLKPLLRTDDIVWVHDYHLIPLAAELRALGCNQRIGFFLH
                     IPLPPPLILAAIPGHDWLIRALFSYDLVGFQSKADHSHFCSYVQTEAHAQELGEGRWR
                     AFNRTVQAGAFPIGIDVEEFTALAEAPEGREMYERMRREYSRRNLLVGVDRLDYSKGL
                     PQRMRAFRDMLQRYPDTHNSATLIQIASPSREDVSAYTDILHELESLCGSINGNFGEL
                     DWMPVRYMHRTVARSRLPGLYRASRAALVTPLRDGMNLVAKEYVVSQNPEDPGVLVLS
                     RFAGAAEQLREALLVNPYDIEGTANAIHLALQMPLVERRERHQALMTTIRRSDVHWWC
                     DGFLDTLAKAQAEETGTPWLRL"
     gene            complement(2977..4779)
                     /locus_tag="VGA59_06135"
     CDS             complement(2977..4779)
                     /locus_tag="VGA59_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX9719293.1"
                     /translation="MNASVEPARPSLNLGVIGNCAYSALVDERGRIVWCCLPRFDGDP
                     VFNALLDPSEAGSVWTFEIEDFERSEQDYKPNTAILRTRLYDRQGQGVEITDFAPRFN
                     SRDRLFRPLTLIRRVKVLSGTPRVRVVMRPRFDWGRQLPTLTQGSHHIRYVGTSQILR
                     LTSDAPLNYLLAETFFVVDRQMNFILGPDETLMGGIEETARLFEQETASYWTTWARRL
                     AIPLEWQEAVIRAAITLKMSLFEDTGAIVAAMTTSIPEAAGSGRNWDYRFCWLRDAFF
                     VVRALNSLSEVGTMEDYLRWLNNIVVRSRGGHIQPLYGIGQEEHLPEAILGHLPGYRG
                     QGPVRVGNQAQEHFQHDVYGNIILGASQSFHDHRLFRRAGRAEFGYLEAVGEQAFRVY
                     DQPDAGMWELRTRARIHTSSALMCWAACDRLAKVAATLKLPERIRHWRERADTVRERI
                     LREAWSEQRQSFAESFGGRDLDASALLMAEVGFIDPNDARFIATVDALERSLCDGPYM
                     RRYEAPDDFGRPETAFNICTFWRIDALARIGRCGQAREIFEVMLKSRNPLGLLSEDTH
                     PVTGEMWGNFPQTYSMVGLINAAVRLSAPWDTVI"
     gene            complement(4784..5518)
                     /gene="otsB"
                     /locus_tag="VGA59_06140"
     CDS             complement(4784..5518)
                     /gene="otsB"
                     /locus_tag="VGA59_06140"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX9719294.1"
                     /translation="MNFVDILCPSCALFLDFDGTLVDLAPQPDAVIVPSGLVHTLDGL
                     NKYLGGALALISGRPIEQIDAFLHPLRLPVAGVHGTERRAFDGAVGLMATHPLQRVED
                     AALALAARHPGLRVENKRGSVALHYRQSPELEPLCLEAMQRAVEESPGLTLLRGKMVF
                     EAKPGGASKGHAIEAFLHEPPFAGRMPIFVGDDFTDEVGFSTVQRLQGLGVKVGEGAT
                     VAWHRLESPAALRYELQRAVAAKAPM"
     gene            5742..6536
                     /locus_tag="VGA59_06145"
     CDS             5742..6536
                     /locus_tag="VGA59_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9719295.1"
                     /translation="MDYSRYHTLAVTRRGPDDTVLDIQMRALNGKLPTAGHEGHLELA
                     QIWRDVGGDETVRCAVLRGEGAGFSGGGDLALVEDMAADFEVRTRVWKEARDLVYNVI
                     NCDKPIVSAIHGPAVGAGLVAGLLADISVAAKSARIVDGHTRLGVAAGDHAAIVWPLL
                     CGLAKAKYYLLLCEAVSGEEAERIGLVSLAVDEDRLLPMAYEIADRLALGSQSAIRWT
                     KYSLNNWLRQAGPAFDTSLALEFMGFGGPDVTEGIAALRQRRAPRF"
     gene            6588..7106
                     /locus_tag="VGA59_06150"
     CDS             6588..7106
                     /locus_tag="VGA59_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX9719296.1"
                     /translation="MNTTFRTCMAFALMAVAVGAFAQAQQPPKVEGGWVRSSVPGQQG
                     TGAFMRLTATEPLQLVGAATPVAGVAELHEMRMEGDVMRMRAVDKLDLPAGRTVELKP
                     GGYHLMLMDLKQPLPQGSSVPLTLLFRNAKGVDSKLELKLPVAIRPPGRGGGAQPKAR
                     AMDRQSTDAHKY"
     gene            7180..7881
                     /locus_tag="VGA59_06155"
     CDS             7180..7881
                     /locus_tag="VGA59_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HEX9719297.1"
                     /translation="MSNNDTFGFGKFVPGFDFLQNLAKGASQTLPQMPNLSNWVAPTL
                     NLEELEKRIGELKAVHFWLDQNSKALGATIQALEVQKMTLATLKGMNFNMGDVANALK
                     LKAADTVFAGVQKVGEKAADAARTVSDAASGAAARSKATRKGARASPPAGGVVDPLQW
                     WSALTQQFQQIAATAMKDAAAQTAVDTARNVATGVAKEAMKTATGMARSATGKMTGGA
                     RKTPAKRPAKKAAGH"
     gene            7906..9171
                     /locus_tag="VGA59_06160"
     CDS             7906..9171
                     /locus_tag="VGA59_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX9719298.1"
                     /translation="MKLFPFGHATHPQWQMAAGLVLAQLRARMALGDHAHAPTLGLLY
                     ITDHYAGDAQEILDHLGAELPEVTDWSGTVGVGIASSNVEYFDEPALAVMLCELAPDQ
                     YRVFSGVAPLAVGEGMGFEPYTALVHADASTPDVSELIAEMAARTATGYLFGGLASSR
                     AGAVQFAVGGNGNIKGQGAAGGVFSGGLSGVAFGEGISLVSRVTQGCQPVAAQRVITA
                     AQGNLITGLDGKPALDVLLGDTGISLDEPEAAMAALRATLVGLTEAGGDAVGRTGNFG
                     ADVVVRHIIGLDPGRRGVAVADRVATGMRMAFCRRNVQAARADLVRICAEIREELEPE
                     EMPLPMAASLAASEAQPGPGASRRIAGAVYVSCAGRGGPHFGGPSAELQIVRHALGDV
                     PLVGFFAGGEIARHHLYGYTGVLTVFTQA"
     gene            9168..10289
                     /locus_tag="VGA59_06165"
     CDS             9168..10289
                     /locus_tag="VGA59_06165"
                     /inference="COORDINATES: protein motif:HMM:NF016293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HEX9719299.1"
                     /translation="MTTGYRMPGAWQGVRWALQPGAEGGGTSRLRLAWRALRAALWHG
                     KAMRRWMAVVVELHSRGIVREVAGEYLRAVRPYVHRGTTYGERVVQLIDHADWLETAF
                     QPSAFGQLASGRPLVLAELAAPRGYDFMRLQLQQAPTQSPEGELLLTLSLQRSADVQH
                     RPQPVDAGVLGFSRFRVDGVACLVIGGMRGQRHPVVRVSTMEVSQALQGWKPAVLMVR
                     VAQELARHWSLHLIGLHPGAHRLHGWSYRWTRRNREAARRIVASYDALWEHFEAGKGP
                     PGWVIVPLNSDEKLAATALSPEKRQRQTRRADYWIRTRNLLRLQFKQLLQRPGREARL
                     SRVTEGMDRESMADDDEDFPASQDMVPSRVLQTGPGSLL"
     gene            complement(10296..11027)
                     /locus_tag="VGA59_06170"
     CDS             complement(10296..11027)
                     /locus_tag="VGA59_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9719300.1"
                     /translation="MSTTADLILALKKELKSAQMTYADLAHSLEMAESSVKRMLAKGD
                     MPLSRIDAICRALKLDFAELARRVADAQPLLKELTQEQERAVVADKKLLLCAICVLSQ
                     WTLDQITSHYRLGEAECIKYFAQLDRIGIIELRPLNRYRLKLAKTFRWRPHGAVMNYF
                     RDHAVLDYFAGGFDGAGEGVLLVHGSVSRGVAPSFLDRMQRVAQDFAQQHQADQKLPE
                     SEREGYTLLLAMRSWEFAAFTALRR"
     gene            11150..11830
                     /locus_tag="VGA59_06175"
     CDS             11150..11830
                     /locus_tag="VGA59_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719301.1"
                     /translation="MQTMFSRREIGLLLAAIGLLALAVLGPTLAQPAHLNDYADQRTL
                     WGLPFALDVLSNLPFALMGAAGIGALVRAPAGAPARVERTMAGLFFAGLVLTAGASGW
                     YHLAPDDMGLAVDRLGMSVAFAGLLGLAAAGRVSGRAGAAVGAAVLVLAPAGVQVWSA
                     TGNVLPWAVVQFGGMGLILWLAALRPVAGTLEVRWGLVILAYALSKLLEVNDPGVYEL
                     TGQLVSGA"
     gene            11988..13910
                     /locus_tag="VGA59_06180"
     CDS             11988..13910
                     /locus_tag="VGA59_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9719302.1"
                     /translation="MSSTVAGLPAPMQAEAHPNQFALLKQRRFAPFFWTQFSGAANDN
                     LFKFAFTVMVTYQLQIGWLPPAMAGLVIGALFILPFLLFSATSGQLADKFDKTTMIRF
                     VKNLEIAIMLIAAWGFYTDNVPVLLACTFLMGLHSTLFGPVKYAYLPQVLGERELTGG
                     NGMVEMGTFVAILLGNVVGGLIVSIPEVGRQDVAIACVLLALVGRAVAQFIPSAPATD
                     PGLKINWNPVSETISNLKLAHGNLVVFRSVLGISWMWFFGAVFLSQFPSFAKEVLHGN
                     EQVASLLLVVFSVGIGIGSLLCEVLSRRHVEIGLVPLGAIGMSVFAVDLYFASRGLPP
                     AAGLTLGQFLATPAHWRVMADLALLSLFAGLYSVPMYALIQLRSQPTHRARIIAANNI
                     LNALFMIASAVIAGVLLKLGMTIPQIFLLVGLANAVVAFYIFMLVPEYLLRFIAWLAS
                     RLVYRFKVTGDENIPVEGAAVLVCNHVSFVDAVLLMAASPRPIYFVMDHRIFTVPVLG
                     WLFKLAKAIPIAPRKEDPAAYEAAFEAAAKVLREGDLLAIFPEGGITHDGKLQQFKGG
                     IMKILEREPVPVIPMALTNLWGSFFSRIERGGAMVRPFRRGLLNRVGLNVGPAVPPAQ
                     VQPEILRHHVAQLLAA"
     gene            14132..14575
                     /locus_tag="VGA59_06185"
     CDS             14132..14575
                     /locus_tag="VGA59_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719303.1"
                     /translation="MKKHLFAAVLSLGALLAGPVRAQSEASAVSALSVLPLASAVAGA
                     SAAAGASVAAPVILSTAGAVFVVKAVEVSALGTVYVLERASDGAHVSIEIAGRGIANA
                     SIVAGTVVTASVIGAGVILSAAGVAVAFVPNALGRALLHNERLTW"
     gene            14583..15386
                     /locus_tag="VGA59_06190"
     CDS             14583..15386
                     /locus_tag="VGA59_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2145 domain-containing protein"
                     /protein_id="HEX9719304.1"
                     /translation="MKNTAIVAAVAFVALASGAAHAGRSCEAKKPSAQTIERGLNLAQ
                     RTMAALDASGAKVVVLARAGQDLSKYGLRYSHLGFAYRQAGADGSGSAWRVVHKLNQC
                     GTADSAVYRQGLGEFFLDDLWRYEAAWVVPSRETQDKLLPLLQDNSRIAVLHHKPYSI
                     VSYAWGRRYQQSNQWAIETMAAAMEDGAPSRERAQSWLRSAGYEPATLKLGPLTRLGA
                     RVSAANVAFDDHPDEKRFSDRIETVTVDSVFQWLQRAGLGAALVTLRLP"
     gene            complement(15407..15829)
                     /locus_tag="VGA59_06195"
     CDS             complement(15407..15829)
                     /locus_tag="VGA59_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9719305.1"
                     /translation="MTRNLETVQAIYQAFGQGDVPGILSKLAPQVQWEAWPDHSAQAA
                     GHPLFAPRSDPQGVAEFFRLVGEQVGIHEFKVLDIFGDGRQVAAEVVMDYSYQPTGRR
                     IRDEELHLWTFGEDGKVTRFRHYIDTAKHLRAAGLLAA"
     gene            complement(15899..17455)
                     /locus_tag="VGA59_06200"
     CDS             complement(15899..17455)
                     /locus_tag="VGA59_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX9719306.1"
                     /translation="MALTANPALRLAEVLGALSLATDLANGQPSEHALRTAILAVRLA
                     QDEPADVRRDVYWAGLLRYLGCSGFAVEEAAYASGDDIGLRVSFIRADLGRPSEFIGA
                     VLRDVGRGAPPLQRAKGVLRLLSDPHAPRAHALAQCDAAEHCGRKLGMPAGVLQALAH
                     SDERYDGRGFPGGLAGDALSLPQRYVEIARVAVVFHALSGVQAARAELKRRAGGHLDP
                     HIVARFADAAPQLCAGLEQDSVWDDFLATEPGLWLLDEDALEPLFEAFALMADLKSGY
                     FSGHSPGVAALARAAAQAQGLAPEQVDLLGRAALLHDLGRVAVSTGLWDKPGPLTAAE
                     WERVRLHSYYTDRVLRHGPALARYADIAGRAHERLDGSGYHRGDRDANMPVRLLAAAD
                     VYQACTEPRAYRPARDANGARQVLLEGVAQGRLCRHAVSGVLSAAGQVPARQASTSET
                     LTDRELEVLRLLVLGLTNKQIGRQLGISPRTVQHHTIHIYGKTGMKSRAGAALWAVER
                     GLLRDAADRR"
     gene            complement(17483..>17691)
                     /locus_tag="VGA59_06205"
     CDS             complement(17483..>17691)
                     /locus_tag="VGA59_06205"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HEX9719307.1"
                     /translation="PDGATSGGFSRAYLHHLERCGEMLAPMLATIHNLHYYLNLMREI
                     REALDAGRFAAFARQFKQDRARGI"
BASE COUNT         2817 a         5911 c         6096 g         2867 t
ORIGIN      
        1 gggcagctgg tcgtggtctc gcgcgacctg ggcacggcgc attatgcgac cggcatcgcg
       61 gaccgcctcc agcaggtgct ggacgactgg agtttcctgt cgccgcaatt gcaggacctg
      121 tacgagaccc tgaacggcgg caaggctcgc cacgccttcc cgttcgaatc cgagcgctgc
      181 atggcgccgc tgccgcgcgc ctgccaatgg gccgacggct cggcgtatat caaccatgtg
      241 gagctggtgc gcctcgcgcg gcatgccgag gtgcccgcca actactacac cgagccgctg
      301 atgtaccagg gcggcagcga cgatttcctc ggacctcagg atgacgtgat cgtgcccagc
      361 gaggagtacg gcatcgactt cgaagccgaa gtcgcggtgg tcaccggtga catcgccatg
      421 acggccacgc ccgatcaggc gctggagggc atccgcctgg tgatgctggc caacgacgtg
      481 tcgctgcgca acctgatccc cgacgaactg gccaaaggtt tcggcttttt ccagagcaag
      541 cctgcgacag cctttagccc ggtggccgtc acgctggacg aactgggcga tgcctgggac
      601 aaaggcaggc tgcacctcac gctgcaaagc acctggaacg ggcgcaccgt gggcatgtgc
      661 gacgccggca aggaaatgac cttccacttc ggccagctga tcgcccacat ctgcaagacc
      721 cgcaatgtgc gcgccggcag cattatcggc tcgggcaccg tgagcaaccg cgccaccgag
      781 gccgatggcc ggaaggaatg gaccaagggc tacagctgca tcgccgaaaa gcgcgccatc
      841 gaaaccatca tggacggcaa gcccgctacc gagtacatga agttcggcga caccatccgc
      901 atcgagatga agggccggga cggccagagc gtgttcgggg cgatcgacca gaagatcgcg
      961 ccgttgaagc gctgaggccg accgcgcccc gcatggagat ggcgagcgag cccctggtga
     1021 tgaacgatcc gcacaacctg ccgcgttttc tggaggccca ggcgcccgtc tatgaccagg
     1081 tgctggcgga actcgcggac ggccgcaagc aaagccactg gatatggttc atctttccgc
     1141 agctcgccgg cctgggccac agcgcgatgg cgcgccggtt cgcgatcgcc tcgctggacg
     1201 aagcggcggc ctatctcgcg catccggtgc tcggagccag attgcgcgag tgcagccgcc
     1261 tggtgctgga cctcgaggag cgctctgtgc acgacatctt cggctcgccg gatgacctca
     1321 aattccgttc ctcgatgacg ctgttcgacc tcgccggtcc ggccgagccg gtgttccggc
     1381 agtgcctgct caagtatttc aaaggccagg gcgatcccca aacgctggcg ctgctgtaaa
     1441 gcgtggcgtg gcgtggtggc ggtggcggtg gcgcggcgct acaggcgcag ccagggcgtg
     1501 ccggtttcct cggcctgcgc cttggcgagc gtgtccagaa agccgtcgca ccaccagtgc
     1561 acgtcgctgc gccggatcgt cgtcatcagc gcctggtgcc tttcgcgacg ctctaccagc
     1621 ggcatctgca gcgcgagatg gatggcattg gccgtgccct cgatgtcgta gggattcacg
     1681 agaagtgcct ccctcagctg ttcggcagcg ccggcgaaac gcgacagcac cagcacgccg
     1741 gggtcctcgg ggttctgaga taccacgtac tccttggcca ccaggttcat gccgtcgcgc
     1801 agcggggtca ccagcgccgc tcggctggcg cgatagagcc cgggcagcct ggagcgcgcc
     1861 accgtgcggt gcatgtagcg caccggcatc cagtcgagtt caccgaagtt gccgttgatg
     1921 gagccgcaca ggctttccag ctcgtgcagg atgtcggtgt aggcgctgac gtcttcacgg
     1981 ctgggcgatg cgatctggat cagggtcgcg ctattgtggg tgtcgggata cctttgcagc
     2041 atgtcgcgga aggcccgcat gcgctgcggc aggccctttg aatagtcgag ccggtccacg
     2101 cccaccagca ggttgcgccg cgagtattca cgccgcatgc gttcgtacat ttcccgccct
     2161 tccggcgcct cggccagcgc cgtgaattcc tccacgtcga tgccgatcgg aaaggcaccg
     2221 gcctgcaccg tgcggttgaa cgcgcgccac cgtccttcgc ccagctcttg cgcatgggcc
     2281 tcggtttgta catagctgca gaagtgcgaa tggtcggctt tgctctggaa gccgacgagg
     2341 tcgtatgaaa acagcgcgcg gatcagccag tcgtgtcccg gaatcgccgc caggatcagc
     2401 ggcggcggca gcggaatgtg caggaaaaag ccgatgcgct ggttgcagcc gagcgcacgc
     2461 agctccgccg ccagcgggat caggtggtag tcgtgcaccc atacgatgtc gtcagtgcgc
     2521 agcaacggct tgagcttgcg cgcgaacagc tggtttacgc ggcggtaccc ggcgatgtag
     2581 ccggcgtcga agtcggccag gtccagccgg taatggaaca ccggccacaa cacgccgttg
     2641 gagtagccca cgtagtaggc agcgtggtcc tcgcggctca ggtccagggt gaccagccgc
     2701 accggcccgg cctgctggct gtgcagctcg ccctcaccgg gcttgccgcc ggccgcgtcc
     2761 tcgacgatct tgccgctcca gccgaaccac aggccgcccg tgttgctcaa gacttccgcc
     2821 agcgccaccg ccaagccgcc tgccgctgtc ttgcggggat ccgccacgcg attggagacg
     2881 acgaccaggc ggctcatggc gtttagcgtt gggcgtcgag cgtcgagcgt gaatttcccc
     2941 tcccgcttat cgctgaaccc tcaatgctcg acttcttcat atgaccgtgt cccagggcgc
     3001 cgacaaccgc accgccgcat tgatcaggcc gaccatcgaa taggtctggg ggaagttgcc
     3061 ccacatctcg ccggtcaccg gatgggtatc ctccgacagc aggcccaggg gattgcggct
     3121 cttgagcatg acctcgaaga tctcgcgcgc ctgcccgcag cggccgatgc gcgcgagcgc
     3181 gtcgatgcgc cagaaggtgc agatgttgaa ggcggtttcc ggcctgccga aatcgtcggg
     3241 ggcctcgtag cgccgcatgt aggggccgtc gcacagcgac ctttcgagag cgtccacggt
     3301 ggcgatgaag cgcgcatcgt tggggtcgat aaaccccacc tcggccatca gcagggcgct
     3361 ggcgtcaagg tcgcggccgc cgaagctctc ggcgaaggac tggcgctgct cgctccaggc
     3421 ctcgcgcagg atgcgctcgc gcacggtgtc ggcgcgctcg cgccagtggc ggatgcgctc
     3481 gggcagcttc agggtcgcgg ccaccttggc caggcggtcg caggcggccc agcacatcag
     3541 ggcggacgag gtgtggatac gagcgcgcgt gcgcaattcc cacatgccgg catcgggctg
     3601 gtcgtacacg cgaaacgcct gttcgcccac ggcctcgagg tagccgaact cggcgcggcc
     3661 ggcacggcgg aacagccggt gatcgtggaa cgactgcgag gcgcccagga tgatgttgcc
     3721 gtacacatcg tgctggaagt gttcctgggc ctggttgccc acccgtaccg gaccctggcc
     3781 gcggtagccg ggcaggtggc ccaggatggc ctcgggcaaa tgctcttcct ggccgatgcc
     3841 gtacagcggc tggatatgcc cgcccctgga gcgcaccacg atgttgttga gccagcgcag
     3901 atagtcttcc atggtgccga cttccgacag gctgttgagc gcgcgcacca cgaagaaagc
     3961 gtcgcgcagc cagcagaagc ggtaatccca gttgcgcccg ctccccgctg cttccgggat
     4021 gctggtggtc atggcggcga cgatcgctcc ggtatcctcg aacaaggaca tcttgagcgt
     4081 gatcgcggcg cggatcacgg cctcctgcca ctccagcgga atcgccagac gccgcgccca
     4141 ggtcgtccag tagctggcgg tctcctgctc gaacaggcgg gcggtttcct cgattccgcc
     4201 catcagggtt tcgtcgggtc cgaggatgaa gttcatctgg cggtcgacga cgaagaaagt
     4261 ctcggccagc aggtagttca gcggggcgtc gctggtcagt cgcagaattt gcgaagtgcc
     4321 gacatagcgg atgtgatggc tgccctgggt gagcgtgggc aactggcgcc cccagtcgaa
     4381 gcgcggccgc atcaccaccc gcacgcgcgg cgtgccggac aggaccttca cgcgccgtat
     4441 cagcgtgagc ggccggaaca ggcggtcccg gctgttgaag cgcggagcga aatcggtgat
     4501 ctcgacgccc tggccctggc ggtcatacag ccgcgtgcgc aggatcgccg tgttgggctt
     4561 gtagtcctgt tcgctgcgct cgaagtcttc gatctcgaac gtccagacgc tgccggcttc
     4621 gctcgggtcg agcaaggcat tgaacaccgg gtccccgtcg aagcgcggca ggcagcacca
     4681 gacgatgcgc ccgcgctcgt cgaccagcgc gctgtaggcg cagttgccga tcacccccag
     4741 gttgagggac ggccgcgcgg gttcaaccga ggcattcatg cgctcacatt ggcgctttgg
     4801 cggccaccgc cctttgcagt tcgtaacgca gcgcggcggg cgattccagg cggtgccacg
     4861 ccacggtcgc cccttcgcct accttgacgc ccagcccctg cagccgctgc acagtggaaa
     4921 agccgacctc gtcggtgaag tcatcgccca cgaaaatcgg catgcggccg gcaaagggcg
     4981 gctcgtgcag gaaggcttcg atcgcgtggc ccttgctcgc cccgccgggc ttggcctcga
     5041 acaccatctt tccgcgcagc aaggtcaggc cgggcgactc ctcgaccgca cgctgcatgg
     5101 cctcgaggca caacggctcg agctcgggtg actgccggta atgaagcgcg accgagccgc
     5161 gtttgttctc gacgcgcagc cccgggtggc gcgccgccag cgccagcgcg gcatcttcca
     5221 cccgctgcaa tggatgggtc gccatcaggc cgacggctcc gtcgaaggcg cggcgctccg
     5281 tcccatgcac gccggccacc ggcaaccgca acggatgcag gaaagcgtcg atctgctcga
     5341 tcgggcggcc cgaaatcagg gccagtgcgc cccccaggta cttgttcagc ccgtccagcg
     5401 tgtgcaccag gccggaaggc acgatcaccg catccggctg gggcgccaag tccaccagcg
     5461 taccgtcgaa gtcgaggaac aacgcacacg acgggcaaag gatatccacg aaattcatgt
     5521 tgaccgaccc tagcagagcc tgggcacgag cactagacgg gccgtgccac tgaaaacgta
     5581 ggaccaagcc tacggcctga cgggaatgat gactcaaaac cggacgggcg caggcgtgga
     5641 tgaaatattc ttctggtggc aaccggtggt cgcgacgcca agacccaagt ggcataagct
     5701 tcgcatcttt tgagggggct gcatgacagc cccatgccat catggactac tcacgctacc
     5761 acacgcttgc cgtgacgcgc cgcggacccg acgacaccgt gctcgatatt cagatgcgcg
     5821 cgctcaacgg caagctgccc acggccgggc acgagggcca cctggaattg gcgcagatct
     5881 ggcgcgacgt cggcggcgac gagaccgtgc gctgcgcggt gctgcgcggc gagggcgcgg
     5941 gattctcggg cggtggcgat ctcgcgctgg tcgaggacat ggcggccgac ttcgaagtgc
     6001 gcacgcgggt ctggaaggag gcgcgggatc tggtttacaa cgttatcaat tgcgacaagc
     6061 ccatcgtcag cgccatccac gggccggccg tgggagcggg gctcgtggcc gggctgctgg
     6121 ccgatatctc ggtcgcggcc aagagcgcca ggatcgtgga cgggcacacc cgcctgggtg
     6181 tggcggccgg tgaccatgcg gccatcgtgt ggcccctgtt gtgcggcctg gccaaggcca
     6241 agtactacct cctgctgtgc gaggccgtga gcggcgagga ggccgagcgg atcggcctgg
     6301 tttcgctggc cgtggacgag gaccggctgt tgccgatggc ctatgagatc gcggaccggc
     6361 tggccctggg ttcccagagc gcgattcgct ggaccaagta ctcgctgaac aactggttgc
     6421 gccaggccgg gccggcgttc gacacttcgc tggcgctcga gttcatgggc ttcggcggcc
     6481 cggatgtgac ggaaggcatc gcggcgctgc gccagcgccg cgcgcccagg ttctaaagtc
     6541 cgggcattgc cggaccgcga taatgcgacc catcgggaat gccgctcatg aacaccacct
     6601 ttcgaacctg catggccttc gccctgatgg cggtcgccgt gggcgcgttc gcccaggccc
     6661 agcagccgcc caaggtggag ggcggctggg tgcgcagctc cgtgcccggg cagcagggta
     6721 ccggcgcttt catgaggctc acggccacgg agcccctgca gctggtgggc gccgcgacgc
     6781 cggtggcggg cgtggccgaa ctgcatgaga tgaggatgga gggcgacgtc atgagaatgc
     6841 gcgccgtgga caaactcgac ctgcccgccg gacggaccgt ggaactcaag ccgggcggct
     6901 atcacctcat gttgatggac ctgaagcagc cgctgccgca gggcagcagc gtgccgctga
     6961 ctctcctgtt ccgaaatgcc aaaggcgtgg acagcaagct ggaactgaag ctcccggtcg
     7021 ccatccgccc tcccggccgc ggcggcggcg cgcagccgaa ggcgcgggcg atggatcggc
     7081 agtccacgga tgcgcacaaa tactgatgcc ggcggcgtcg ccagccgcgg gcggttcagg
     7141 taagctgtca gcacaagaca accccaagcg gagcgcggca tgagcaacaa cgacaccttc
     7201 ggtttcggca aattcgtgcc cggtttcgat ttcctgcaga acctggccaa gggcgcttcg
     7261 cagacattgc cgcagatgcc caatctgtcc aactgggtgg cgccgacgct gaacctcgag
     7321 gagctggaaa aacgcatcgg cgaactcaag gccgtgcatt tctggctgga ccagaattcc
     7381 aaggcgctgg gcgccaccat ccaggcactg gaagtgcaga agatgacgct ggccacgctc
     7441 aagggcatga acttcaacat gggcgacgtc gccaatgccc tgaagctcaa ggccgccgac
     7501 accgtgttcg ccggcgtcca gaaggtcggc gagaaggcgg ccgatgcggc caggaccgtc
     7561 tcggatgcgg cctcgggcgc ggcagccagg agcaaggcga cgcgcaaggg cgcccgggcg
     7621 tcgccgccgg ccggcggcgt cgtcgatccg ctccagtggt ggagcgcgct cacccagcag
     7681 ttccagcaga tcgccgccac cgccatgaag gatgccgcgg cccagaccgc ggtggatacg
     7741 gcacggaacg tggcgaccgg cgtcgccaag gaggcgatga agaccgccac cggcatggcc
     7801 aggtccgcga ccgggaaaat gaccggtgga gctcgcaaga cgccggccaa gcggccggcc
     7861 aagaaggcgg ctggtcactg acgccgccat cccttcgagg aaaccgtgaa gctctttccc
     7921 ttcggccacg ctacgcatcc gcagtggcag atggcggcgg ggctggtgct cgcgcaattg
     7981 cgcgcccgga tggccttggg cgaccacgcc cacgcgccga cgctgggcct gctctacatc
     8041 accgaccact acgcaggcga cgcgcaggag atcctggacc acctgggcgc cgagctgccc
     8101 gaagtcaccg actggtccgg gaccgtgggt gtgggaatcg cgtcctccaa cgttgaatac
     8161 ttcgacgaac ccgcgctggc ggtgatgctg tgcgagcttg cgcccgacca gtaccgggtg
     8221 ttctccggcg tggcgccgct ggccgtgggc gagggcatgg gtttcgagcc gtataccgcg
     8281 ctggtccatg ccgacgcgag cacgccggac gtttcggaac tcatcgcgga gatggctgcg
     8341 cgcaccgcca ccggttacct gttcggtggc ctggcatcca gccgcgccgg cgcggtgcag
     8401 ttcgcggtgg ggggcaatgg caacatcaag ggtcagggcg cggccggcgg cgtgttcagc
     8461 ggtggcttgt ccggcgtggc cttcggcgag ggcatctcgc tcgtttcgcg ggtgacgcaa
     8521 ggctgccagc cggtggcggc gcagcgggtg atcacggcgg cgcaaggcaa cctgatcacc
     8581 ggactggacg gcaagccggc gctggacgtg ctgctgggcg acaccgggat ctcgctggac
     8641 gagccggagg cggcgatggc cgcgctgcgc gccacgctgg tcggattgac cgaggccggc
     8701 ggcgatgcgg tcggccgaac cggaaatttc ggcgccgacg tcgtggtgcg ccacatcatc
     8761 gggctggacc ccggacggcg cggcgtcgct gtcgccgatc gcgtggcgac gggcatgcgc
     8821 atggccttct gccggcgcaa tgtgcaggcc gcgcgcgccg acctggtgcg aatctgcgcg
     8881 gagatccgcg aggaattgga acccgaggag atgcccctgc cgatggccgc ctcccttgcc
     8941 gcgtccgaag cccagcccgg gcccggcgcg agccggcgca tcgccggcgc cgtctacgtc
     9001 agctgtgccg gccgcggcgg cccgcatttc ggcggcccca gcgcggaatt gcagatcgtg
     9061 cggcacgcgc tcggcgacgt gccgctggtg ggcttctttg ccggcggcga gatcgcccgc
     9121 catcaccttt acggctacac gggagtgttg accgtgttca cgcaagcatg acgaccggtt
     9181 accggatgcc gggagcttgg cagggcgtgc ggtgggccct gcaaccgggc gccgaaggcg
     9241 gcggcacctc gcggctgcgc ctcgcgtggc gagccttgcg cgccgcactg tggcacggca
     9301 aggcgatgcg ccgctggatg gcggtggtgg tcgagctgca ttcgcgcggc atcgtcaggg
     9361 aggtggccgg cgaatacttg cgtgccgtgc ggccctacgt tcatcgcggc accacctacg
     9421 gcgagcgcgt ggtgcagctg atcgatcacg cggactggct ggagacggcg ttccagccgt
     9481 ccgctttcgg gcaactggct tccggccggc cgctggtgct ggccgaactg gccgccccgc
     9541 gcggctacga tttcatgcgg ctgcagctgc agcaggcgcc gacgcaaagc cccgagggcg
     9601 agctgctgct cacgctcagc ctgcagcgct ccgccgacgt gcagcacagg ccgcagccgg
     9661 tggacgcggg cgtcctgggc ttcagcaggt tccgtgtcga cggcgtggcc tgcctggtga
     9721 tcggcgggat gcgcggccag cgccatccgg tggtgcgcgt gagcacgatg gaagtgagcc
     9781 aggcgctgca gggctggaag ccggcggtgc tgatggtgcg ggtggcgcag gagctggcgc
     9841 ggcattggag cctgcacctg atcggccttc atcccggcgc gcaccggctg catggctgga
     9901 gctaccgatg gacccggcgt aaccgcgagg ccgcccggcg catcgtcgcc agctacgacg
     9961 cgctgtggga acatttcgag gcgggcaagg gcccgcccgg ctgggtgatc gtgccgctca
    10021 actccgacga gaagctcgcg gccacggcgc tctcgcccga gaagcgccag cgccagacgc
    10081 gccgcgccga ctactggatt cgcacgcgta acctgctgcg cctgcagttc aagcagttgc
    10141 tgcagcggcc cggccgcgaa gcgcgcctgt cgcgcgtgac cgagggcatg gatcgcgaaa
    10201 gcatggccga tgacgatgag gactttccgg ccagccagga catggtgccc tcacgcgtgc
    10261 tgcagaccgg gcccggcagc ctgctgtgag ggccgtcagc gccgcagcgc cgtgaacgcg
    10321 gcgaactccc agctgcgcat cgccagcagc agcgtgtagc cttcgcgctc cgactcgggc
    10381 aacttctggt cggcctggtg ctgctgggcg aaatcctggg ccacgcgctg catgcgatcg
    10441 aggaaagacg gcgcgacgcc gcggctcacg gagccatgca ccaggagcac gccctcgccc
    10501 gcgccgtcaa agccgccggc gaagtagtcg agcacggcgt gatcgcgaaa ataattcatc
    10561 accgcgccat gcgggcgcca gcggaaggtc ttggccagct tcagccggta gcgattgagc
    10621 gggcgcaact cgatgatgcc gatgcgatcg agctgcgcga agtacttgat gcactcggct
    10681 tcgccgagcc ggtagtggga ggtgatctgg tccagcgtcc actggctgag cacgcagatc
    10741 gcgcacagca gcagcttctt gtccgccacc accgcccttt cctgttcctg cgtcagctcc
    10801 ttgagcagtg gctgggcgtc ggcgacacgc cgcgccagtt ccgcgaaatc gagcttgagc
    10861 gcgcggcaaa tcgcgtcgat gcgcgacagc ggcatgtcgc ccttggccag catgcgcttg
    10921 acgctggatt ccgccatctc cagcgaatgg gcgagatcgg catacgtcat ctgcgcggat
    10981 ttgagctctt tcttcagggc caggatcaga tcggcagtgg tgctcatggt aggggcaaag
    11041 taccgccaag cgataccggg tagccaggat ctgtgcaatg tacctgattt cggtaccgaa
    11101 gcggccgccg cgccgcagac ttcgcttcaa ttccaacgaa ggagcgatga tgcaaaccat
    11161 gttctcgagg cgcgaaatcg ggctgctgct ggcggcgatc ggcctgttgg ccctggccgt
    11221 gctcgggccg acgctggccc agccggcgca cctcaatgac tacgcggacc agcgcacgct
    11281 gtggggcctg ccgttcgcgc tggacgtgct gtcgaatctg cctttcgcgc tgatgggcgc
    11341 ggccggcatc ggggcgctcg tgagggcacc cgccggcgcg ccggccagag tcgagcggac
    11401 gatggccggc ctgtttttcg ccggcctggt gctgacggca ggtgcctcgg gctggtacca
    11461 cctcgcaccc gacgacatgg gcctggcggt ggatcgcctt gggatgtcgg tggctttcgc
    11521 cggtctgctg ggcctggctg ccgcgggccg cgtgagtggc cgcgccggcg cggcggtggg
    11581 cgcggcggtc ctcgtgctgg cgcccgccgg cgtgcaggtc tggtcggcga ccgggaatgt
    11641 gctgccttgg gccgtggtgc agttcggcgg catgggcctc atcctgtggc tggccgcgct
    11701 gcgcccggtt gccgggacgc tcgaggtccg ctggggcctg gtcatcctgg cctatgcgct
    11761 gtccaagctg ctggaagtga acgaccctgg ggtctatgaa ctcaccggcc agctggtatc
    11821 cggcgcttga agcatgtggt ggcatcgctg gccgcgctgc ctgtcattgg cgcgatccgc
    11881 aggcgagcca ggttcgcgca aaatgccgct tccgccggcg cgaggaacgg cctcgcgcgc
    11941 cgtccggcca gccacgcgta aacaagaact ggaacaggag agactccatg agcagcaccg
    12001 tcgccggcct gccggccccc atgcaggccg aagcgcatcc caaccagttc gcgctgctca
    12061 agcagcgccg ctttgcgccg ttcttctgga cccagttctc cggcgctgcc aacgacaacc
    12121 tgttcaagtt cgccttcacc gtgatggtca cctaccagct gcagatcggc tggttgccgc
    12181 cggcgatggc ggggctggtg atcggcgcgc tgttcatcct gccgttcctg ctgttctcgg
    12241 ccaccagcgg gcagctcgcg gacaagttcg acaagaccac gatgatccgg ttcgtgaaga
    12301 acctggagat cgcgatcatg ctgatcgcgg cctggggctt ctacacggac aacgttccgg
    12361 tgctgctcgc ttgcaccttt ctcatgggac tgcactccac gctgttcggg ccggtgaaat
    12421 acgcctacct gccgcaagtg ctgggcgagc gcgaactcac gggcggcaac ggcatggtgg
    12481 agatgggcac gttcgtggcc atcctgctgg gcaacgtggt gggcggcctg atcgtgtcca
    12541 ttccagaagt gggccggcag gacgtggcga tcgcctgcgt gctgctcgcg ctggtgggcc
    12601 gtgccgtcgc gcagttcatc ccttccgcgc cggcgaccga tccgggcctg aagatcaact
    12661 ggaacccggt cagcgagacc ataagcaacc tcaagctggc gcacggcaac ctcgtggtat
    12721 tccgttcggt gctgggcatc agctggatgt ggtttttcgg cgccgtgttc ctcagccagt
    12781 ttcccagctt cgccaaggaa gtgctgcacg gcaacgagca ggtcgcttcg ctgctgctgg
    12841 tggtcttctc cgtgggcatc ggcatcggct cgctgctgtg cgaagtcctg tcgcgacgcc
    12901 acgtggagat cggcctggtg ccgctgggtg ccatcggcat gagcgtgttc gccgtggacc
    12961 tgtacttcgc ctcgcgcggc ctgcctcccg cggccggcct cacactgggc cagttcctcg
    13021 ccaccccggc gcactggcgc gtgatggccg atctcgccct gctcagcctg ttcgccggcc
    13081 tgtacagcgt gcccatgtac gcgctgatcc agctgcgcag ccagcccacg caccgcgcgc
    13141 gcatcatcgc cgccaacaac atcctcaacg cgctgttcat gatcgccagc gccgtgatcg
    13201 ccggcgtgct gctcaagctg ggcatgacca tcccgcagat cttcctgctg gtgggcctgg
    13261 ccaacgcggt ggtcgcgttc tacatcttca tgctggtgcc cgaatacctg ctgcggttca
    13321 tcgcctggct ggcgtcgcgc ctcgtgtacc gcttcaaggt cacgggcgac gagaacatcc
    13381 cggtcgaagg tgccgcggtg ctggtgtgca accacgtgag cttcgtcgac gccgtcctgc
    13441 tgatggcggc gagcccgcgg ccgatctatt tcgtgatgga ccatcgcatc ttcacggtgc
    13501 ctgtgctcgg ctggctgttc aagctggcca aggcgatccc catcgccccg cgcaaggagg
    13561 acccggcggc ctacgaagcg gcgttcgaag ccgcggccaa ggtgctgcgg gagggcgatc
    13621 ttctggcgat ctttcccgag ggcggcatca cccacgacgg caagctgcag cagttcaagg
    13681 gcggcatcat gaagatcctg gagcgcgagc ctgttcccgt gatcccgatg gcgctcacaa
    13741 acctgtgggg ctcctttttc agccgcatcg agcggggcgg cgcgatggtg cggccgttcc
    13801 ggcgcggctt gctcaaccgg gttggcctga acgtgggtcc tgccgtgccg ccggctcagg
    13861 tacaaccgga aatcctgcgc catcacgtcg cgcaattgct cgccgcgtga gtatcgcttg
    13921 gtgatacccg cggcgcatcg ggagtgcaag cgggcgaaat agcgcgactt ttcgcgcgca
    13981 gccctccaca ctgcctgcat gttcaaccac ctccaggagg tccacatgca agtgcagatt
    14041 cagcgcgatt ccaacgcctg gcaactgcag gcctgggtgt cattcctggt ggccgcgtga
    14101 gcaaccctta gacctttcca aggaacccat catgaaaaaa catctgttcg ccgcggtgct
    14161 ctcgctcggc gcgctcctgg ccggccccgt ccgggcccaa agcgaagcct ccgccgtgag
    14221 cgcgctgtcg gtcctgccgc tggcctcggc ggtcgccggc gccagtgccg cggccggcgc
    14281 gtcggtggcg gcgcccgtga tcctgtccac cgccggcgcc gtgttcgtcg tcaaggccgt
    14341 cgaggtgagc gcgctgggca cggtttacgt gctggaacgc gcgtcggatg gggcgcacgt
    14401 gagcattgaa atcgccggcc gcggcatcgc gaacgcatcg atcgtggccg gcacggtggt
    14461 cactgccagc gtgatcggcg ccggcgtcat cctctccgcg gcgggcgtgg cggtcgcctt
    14521 cgtgccgaac gcgctgggcc gcgcgctgct gcacaacgag cgcctgacct ggtgaggacg
    14581 tgatgaagaa caccgcgatc gtggcggccg tagcctttgt cgcactggcc agcggggcgg
    14641 cgcatgcggg ccgatcctgc gaagcgaaga agcccagcgc ccagacgatc gagcgcggcc
    14701 tgaacctggc gcagcgcacg atggccgcgc tcgacgccag cggcgcgaag gtggtggtgc
    14761 tggcgcgcgc gggccaggac ttgagcaagt acggcctgcg ctattcgcac ctgggcttcg
    14821 cctaccggca ggccggcgcc gacggcagcg gctcggcctg gcgcgtggtc cacaagctca
    14881 accaatgcgg caccgccgac tcggcggtct accgccaggg cctgggcgag ttcttcctcg
    14941 acgacctgtg gcgttacgag gcagcctggg tcgtgccctc gcgcgagacc caggacaagc
    15001 tgttgccgct gctgcaggac aactcgcgga tcgcggtgct gcaccacaag ccctacagca
    15061 tcgtgagcta cgcctggggc cggcgctacc agcaatccaa ccaatgggcg atcgagacca
    15121 tggcggcggc gatggaagac ggcgcgccct cgcgcgaacg ggcgcagtcc tggttgcgct
    15181 ccgccggcta tgagcccgcc acgctcaagc tcggtccgtt gacgcggctg ggcgcccggg
    15241 tcagcgccgc gaacgtggcc ttcgacgacc atccggacga aaagcgcttc agcgatcgga
    15301 tcgagaccgt gaccgtggac tccgtgttcc agtggctgca gcgtgcgggt ctgggagccg
    15361 cactcgtgac cctgcgcctg ccgtgagacc gatcgccggc cgccattcag gccgccagca
    15421 ggccggctgc gcgcaagtgc tttgccgtgt cgatgtagtg ccggaagcgc gtgaccttgc
    15481 cgtcttcgcc gaaggtccac aggtgcagct cttcgtcgcg gatgcggcgg ccggtaggct
    15541 ggtagctgta gtccatgacc acctccgcgg ccacttgccg gccgtcgccg aagatgtcca
    15601 gcaccttgaa ctcgtggatt cccacctgct cgcccaccag gcggaagaat tcagccacgc
    15661 cttggggatc gctgcggggg gcgaacagcg gatggccggc cgcctgcgcg ctgtggtcgg
    15721 gccaggcttc ccactgcacc tgcggcgcca gcttggagag gatgccgggc acatcgccct
    15781 ggccgaacgc ctggtagatg gcttgcacgg tttcgagatt gcgggtcatg gtggctcctt
    15841 ggttcgaggg agcctccacg atagaaaacg cgccgcgatg gcgcatcggc cagatggcct
    15901 atcggcggtc ggcagcgtcg cgcagcagcc cgcgctcgac cgcccacagc gccgcgccgg
    15961 cccggctttt catgccggtc ttgccataaa tgtggatggt gtggtgctgg accgtgcgcg
    16021 gcgaaatgcc tagctggcgg ccgatctgct tgttggtcag gcccagcacc aacaggcgca
    16081 gcacttcgag ctcgcggtcg gtcagcgttt cgctcgtgct cgcctggcgc gcaggcacct
    16141 gcccggccgc gctcagcacg ccgctgaccg catggcggca caagcgcccc tgggcgacgc
    16201 cttccagcag gacctgccgc gcaccgttcg cgtcccgcgc cggccggtaa gcccgtggct
    16261 cggtgcaagc ctgatagaca tccgccgcag caagcagtcg caccggcatg ttggcgtccc
    16321 ggtccccgcg gtgatagccg ctaccgtcca gccgctcatg ggcccgtccc gcgatgtccg
    16381 cataccgcgc gagagccggg ccgtggcgca gcacgcgatc ggtgtagtag ctgtgcaggc
    16441 gcacccgctc ccactcggcc gcggtgagcg gcccgggctt gtcccacaac cccgtgctca
    16501 cggccacgcg gcccaggtcg tgcagcaatg cggcgcgacc gagcaggtcc acctgttccg
    16561 gtgccaatcc ctgtgcctgc gccgctgcgc gtgcaagggc cgccacgccg ggcgaatggc
    16621 cgctgaagta gcccgatttc aggtcggcca tcagggcaaa ggcttcgaac agcggctcca
    16681 gcgcgtcttc atcgagcagc catagaccgg gttcggtggc caggaagtca tcccagaccg
    16741 agtcctgttc caggccggcg cacagctgcg gtgcggcgtc ggcgaaccgc gccacgatat
    16801 gcggatcgag atggccaccg gcacgccgtt tcaattcggc ccgcgcggcc tgcaccccgc
    16861 tgagcgcgtg gaacacgacg gcaacccgtg cgatctccac ataccgctgc ggcagcgaca
    16921 gcgcgtcgcc ggcgaggcca cccggaaagc cgcggccgtc gtagcgctcg tcactgtgcg
    16981 ccagcgcctg cagcactccc gcgggcatgc cgagcttgcg gccgcagtgt tcggcggcat
    17041 cgcattgggc gagggcatgg gcgcgcggag cgtgcggatc ggacagcaag cgcaggacgc
    17101 ccttggcgcg ctgcaatggc ggtgcgccgc ggccgacgtc gcgcagcacg gcgccgatga
    17161 actcgctcgg ccggcccagg tcggcgcgta tgaagctcac gcgcaggccg atgtcgtcgc
    17221 cgctggcata agccgcctct tccaccgcga aaccgctgca gcccaggtag cgcagcagcc
    17281 cggcccagta cacatcccgg cgcacgtccg cgggctcgtc ctgcgccagt cgcacggcga
    17341 gaatggccgt gcgaagcgca tgttcgctgg gctgtccgtt cgccagatcg gtggccagcg
    17401 agagcgcgcc caggacctcg gccaggcgaa gggcagggtt ggcggtgagg gccatgagaa
    17461 cgggcttgcc gagcgcggct tcctatatgc cgcgtgcccg atcctgtttg aactgccgcg
    17521 cgaacgcggc gaagcggccc gcgtccagcg cctcgcgaat ctcgcgcatc aggttcaggt
    17581 agtagtgcag gttgtggatg gtggcgagca tgggcgccag catttcgccg cagcgctcca
    17641 gatggtgcag gtaggcgcgc gagaagccgc ccgaagtggc gccgtcggga g
//
LOCUS       DASWIY010000162        14994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1954221, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000162 DASWIY010000000
VERSION     DASWIY010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954221"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..268)
                     /locus_tag="VGA59_06210"
     CDS             complement(134..268)
                     /locus_tag="VGA59_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719308.1"
                     /translation="MSSARVLGRTRWVIEGERGAALTPGMHPSTLRGRMRKLGIRKAP
                     "
     gene            complement(387..1331)
                     /locus_tag="VGA59_06215"
     CDS             complement(387..1331)
                     /locus_tag="VGA59_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9719309.1"
                     /translation="MAVESGGGRVKRHVLMRHAAFAQRRVARLDPVVRGLLWSMAAGL
                     LFVVLNTLMRSLTLQLDPFQTQFLRYVFGLLVMLPLVARSGLAAYRPRNIGGQFLRGA
                     VHTLGLTIWFTAIPHITLADTTAIGFTGPIFIMIGAAWAFGERMRWERWLAAAIGLAG
                     VLIVVAPKLSGAAGVYTLLMLASIPVFAASFLITKGLTRYERPEVIVLWQSITVTLFS
                     LPLALPGWQAPTAWQWLGFLGCGVLGSAGHYCLARSFGVADISATQSVKFLDLVWASL
                     LGWLVFADAPSRSTLAGGCVIVAATLWIARRESRGERS"
     gene            complement(1486..1995)
                     /locus_tag="VGA59_06220"
     CDS             complement(1486..1995)
                     /locus_tag="VGA59_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9719310.1"
                     /translation="MKILIPVDGSPLSLEAVRHALGLIGQGLRADLVLANVQAPASLY
                     EIVVAHDPDVIKDVSAGAGAHLLEPAEALCRAAGVAYECEVVSGDPVSVLCDLAEEYG
                     CSAIVIGAHGKGAIASALLGSVSHALVHASPVPVTIVRHAEYDQPESEAGSEAPTQSV
                     AEDQSDARR"
     gene            complement(2066..3037)
                     /locus_tag="VGA59_06225"
     CDS             complement(2066..3037)
                     /locus_tag="VGA59_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9719311.1"
                     /translation="MGRRLALADNEGMSNDAVDLRFFVVVTESGSLAEAARRMDVTPS
                     AVSQRLRQLETRLGLHLIHRSTRRFSLTEEGTLFYSRAVELLAQVDDLIDDLRMRSGE
                     VAGTLHVCGPLGFGRHHLAPAIADFHSLHPKLMVSLTLSDVVSVADANRFDLIVHIGE
                     RPDSSVVAYPIAPNARFICASPGYLAHRPAPLAPQDLATHDCIVLRENDEDVTLWRFR
                     KKRSELAVRVPAILSSNDGDVVRQWALQGKGLVMRSEWNVAECLAEGRLVRVLQDWHV
                     PDADIMAMVARREGMAGRVKLFLAFLQARFKPQPPWRQPDRAVQRRA"
     gene            complement(3037..4008)
                     /locus_tag="VGA59_06230"
     CDS             complement(3037..4008)
                     /locus_tag="VGA59_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX9719312.1"
                     /translation="MLVITEQDARALVGMRDAIAAVEHSFAAMARGQARNYPVVREAV
                     GYQDAVFGVKTGCDTQAPVLGLKAGGYWPHNAAKGLTNHQSSTLLFDPRTGRASALVS
                     ANYLTGVRTGAASAIATRHLSRPDSTVLGIIGTGVQALHQLEATLAVRSIRTVHAWNP
                     SADKLVAFGRQVRELGLEFAPQADRQAVAANADILITVTPSTRPLVEKSWVRPGTHIN
                     AMGADTKGKQELDPELVASAALFVDKAAQAFSIGECQHAYQAGLITERSLRGSLGAVI
                     AGLCDGRRTAQEITVFDGTGVALQDLAVADLAVRLAGERGLGLRVEY"
     gene            complement(4032..5171)
                     /locus_tag="VGA59_06235"
     CDS             complement(4032..5171)
                     /locus_tag="VGA59_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX9719313.1"
                     /translation="MAQQSFEQLETPALLLDEARMERNIKRMRSRLRGLGVTFRPHVK
                     TSKSIDVARRLMETPQGPITVSTLLEAEYFASHGVKDILYAVCIAPNKLDHVLALQDR
                     GVRLTLILDCMETARLVAERAHQAKRGFEVLIEIDSDGHRSGVKPDAPELVEIGCYLE
                     RAGVSMRGVLTHAGASYESPSVHAIGEMAERERAAAVHAAQRLRAAGIASPVVSVGST
                     PTALFAEHLEGVTEVRAGVFVFFDLVMAGLDVCRVDDIALSVLATVIGHQREKGWTLL
                     DAGWMAMSRDRGTSRQAVDQGYGVVCDLDGRPLDDFILVDANQEHGIMAHRSGQPDRA
                     AHLPVGTQVRILPNHACSTAAQYGHYKVLGADREVTATWPRFSGW"
     gene            complement(5174..6640)
                     /locus_tag="VGA59_06240"
     CDS             complement(5174..6640)
                     /locus_tag="VGA59_06240"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX9719314.1"
                     /translation="MQDNFDLLIRNASIVDGTGAPRFAGDIGVRGDRIERIGDLAAQR
                     GDVEIDLAGRIAAPGFIDAHTHDDRAMLSNRDMAPKVSQGVTTVIAGNCGISLAPMPR
                     PIPQPVTPPLNLLDDQGEWFHFPTFASYVEGLRANPAATNCALLVGHTTLRVATMDDL
                     EQPASAQEIGRMRALVQEALEAGAIGVSTGLFYEPAIAAPTEEVIETCRPLKEFNGLY
                     CTHMRNEADRVMDSLEETFRIGREVGVPVVISHHKVVGEANYGRSEETLAFIRKSMSQ
                     QPICLDCYPYTAGSTILSADRAAGSGRVIVSWSKTLPEYAGQDLADIARKMGVSQEEA
                     IARLVPAGAIYFRMDETDVQRILKFDQTMIGSDGLPHDASPHPRLWGSFPRVLGHYGR
                     LLGLFSLETAVYKMTGLTARNFGLKDRGVLREGAYADLTLFDADTVDEAATYAVPIAQ
                     SRGIDTVIVNGAVVWRDGASTGAHPGRVLAREALEGGA"
     gene            complement(6791..7522)
                     /locus_tag="VGA59_06245"
     CDS             complement(6791..7522)
                     /locus_tag="VGA59_06245"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF005068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEX9719315.1"
                     /translation="MDRNFAEQRKDLAAVFRGAAMFSGQAGVCSHFSLRVNDEPLLFL
                     LNPWGMYFSEARASELMLIDENGVDRQGARDGGFAAFNIHSQIHAIHPEARCVLHTHM
                     PYATAITMLEGGRLEPASQEALRFYGDIAYDDSYNGLAHDQDEGARIARKMDGKHVLF
                     LAHHGVIVVGPSVAQAFDHLYYLERAAELQVKAMSCNRPLRRVPDGIAGKTVQQFGRE
                     REHWARLHLDALRRKLDQEAPDYRD"
     gene            complement(7550..8824)
                     /locus_tag="VGA59_06250"
     CDS             complement(7550..8824)
                     /locus_tag="VGA59_06250"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9719316.1"
                     /translation="MIALGISFVALLLVGVPVLFALGLSGAIGMWAVGVDLISVPTRL
                     FTGMDSFILLAAPFYIFAGEIMNRGGITTRLIRLAGLITRGVHGGTAYTNVTSSVFFA
                     GISGAAVADTAALGQVFIKGMPQEGYDKNYAAAVTIASSIIGPIIPPSVIMIIYAAVT
                     RVSVIDLFVAGIVPGLMLAGACWLVIFIQARGGRLPRSPREKVEKGELLKLVRDGLLV
                     GALPAIIVVGTLSGAFTATEAGGVAVVYALIITWGVFRSIDWPQLWDALQVSARMTAT
                     VYLIIAAATIVSYVLTIGGIADWVQELGTFFKGQPTLFMMALALVLLVVGTFLEPGAA
                     IVLLMPMLLPLSQAMGIDPMQFAMVVILTLTLGLITPPVGVCLFLACRIANSTVGAVV
                     RAVMPFLLAELGVVFLLTLVPALSSGLPSLFK"
     gene            complement(8821..9378)
                     /locus_tag="VGA59_06255"
     CDS             complement(8821..9378)
                     /locus_tag="VGA59_06255"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9719317.1"
                     /translation="MSAVLSQVEARPAAQLRIQTQAFRVGLALSNWADRIAALLLIAV
                     VAMNVAQIIFRYVLIDPLSWSEETMRYSTVWMVLLAGSSALFRGEHMEISIFDKVRSD
                     RVRNAIRLLVLSCIALFCLLLMWEGFRAAIQNIEQRSPAVEIPMVIPYLAIPVGAALM
                     LVKLVCLMALPELAYANQMKKGEGE"
     gene            complement(9389..10414)
                     /locus_tag="VGA59_06260"
     CDS             complement(9389..10414)
                     /locus_tag="VGA59_06260"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9719318.1"
                     /translation="MSKYFKQVLVLVAAAAALGLSTAGYAKTIRFGMNAPKGDNPEWN
                     ALVAFKDHVEYKTEGKIEVKIFPSAQLGAERACAEQVKQGAVEACLVDTGALGGFYKN
                     IQVLSVPYLFKSSNHAWKVFESPFMADLAENMRKQTGIRVMAWGENGFRNFTNNVRPI
                     KSPADLKGLKMRVMESPVFMRFVQSMGAAATPMPGSEIIMAAKSGVIDGQENPAQVIY
                     DYNLADVQKHMSTDEHILGVHAFIMNDQFFNGLTSDEKAVVLEAATLAARIENTQKLA
                     GAKKYIDLIKGKGLKVHMTTMDEKAQFAAVSQEPVLEYLRQQVGADLVKGLLATAKAH
                     EPSLYGR"
     gene            complement(10519..11634)
                     /locus_tag="VGA59_06265"
     CDS             complement(10519..11634)
                     /locus_tag="VGA59_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX9719319.1"
                     /translation="MSPWSAAQPGDRLSAVDTPALVLDLDAFERNMQRMASALNGTGV
                     RLRPHAKSHKCPEIARRQIALGAVGICCQKVSEAAVFVDAGIQDVLVTNEVVGDAKLR
                     RLTALARTARIGVLVDHPQQVQALAAAARVQEVTLDVYVEVDVGAHRCGVAPGEAAVA
                     LARHVASSAPLRFAGLQCYHGPAQHVRSPRGRADAIANAVEAARTTRDAIQACGIRVE
                     RVTGAGTGSFVHERDSGIFNEIQAGSYVFMDRDYGDNQAGAGDIAFEQALFVRTTVMS
                     RPAMNRAVVDAGLKASSVDSGMPSVWRRPDLSFVKAADEHGVLLAAGGAAVSLGESLM
                     LVPGHCDPTVNLYDELVCVRGDTVEAIWPIAARGALL"
     gene            complement(11661..12632)
                     /locus_tag="VGA59_06270"
     CDS             complement(11661..12632)
                     /locus_tag="VGA59_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9719320.1"
                     /translation="MRPRSLLLTVAFAVLAAVSLPGFAAYPDKPIRLIVPSAPGGAPD
                     VLMRSLAAQLSTQMGVPIVIDNKPGGSYVIGTMELVRAPADGYTLAYGNVVSLATNRS
                     LMSNVPYDVDKDLTLVSNTLRVVNLMIVNKDLPVKNVGELVAYARKNPGKIAFASDGN
                     GTTAHLGVELFKSVTGTHMLHVPYRAVSSAITDLIGGGIQLMMVNTPVSGPHVQAGRV
                     KALGISSRQRSTAYPDIPTIAESGVPDFEVVAWGGIVGPAHMPKEIVSRLNAEVRAAL
                     ASPAVRERFKALGAETAPSTPEEFRELSRRETVKWAQVVKYSGARID"
     gene            12978..13196
                     /locus_tag="VGA59_06275"
     CDS             12978..13196
                     /locus_tag="VGA59_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719321.1"
                     /translation="MLNASIRALEGKGEPPSQAEAQAIRRLRAVADDLFQQAMAQMGE
                     MAVMARTAGNGVSRTTGLTPDKLKGTQP"
     gene            13284..14399
                     /locus_tag="VGA59_06280"
     CDS             13284..14399
                     /locus_tag="VGA59_06280"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX9719322.1"
                     /translation="MSKAQLTGYVEPNEETPLATRVEDVRIRDVRPLISPALLQYELP
                     VSEPAQLFVEDSRRKIAALLRGEDPRLLVVVGPCSIHDSQQALEYARRLHALSGQLKQ
                     DLLLVMRVYFEKPRSTVGWKGFINDPRLDGSFHINEGLRRARELLLSIAQMGLPAATE
                     FLDLLSPQYIVDLISWGAIGARTTESPSHRQLASGLSCPVGFKNGTDGGVQMAADALV
                     ACRSPHAFMGMTKMGQVAIFETTGNGDCHIILRGGSRGPNYSPADIDAACATLRKSGV
                     SERVMVDCSHANSAKKYARQITVAQELAAQIADGEHRILGLMIESHLKPGRQDLRVGV
                     PLEPGVSITDGCIGWEQTETVLRQLAEATRQRRARAG"
     gene            14602..14922
                     /locus_tag="VGA59_06285"
     CDS             14602..14922
                     /locus_tag="VGA59_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9719323.1"
                     /translation="MKLRPLHDRVIVKRIDSETKTASGIVIPDAAAEKPDQGEVLAVG
                     PGRKTDKGELIAPNVAVGDRVLFGKYSGQTVKVEGDELLVMKEDDLFAVIEGAPAAGL
                     KKVA"
BASE COUNT         2649 a         5012 c         4901 g         2432 t
ORIGIN      
        1 agacctaaag cagcgcaagt tcggcggcga cttcgacaag ctgctggcct ggtggcagca
       61 gaacaaggcc gcgcagcacg acgcgctgga cagcgcctac cgcgccgccc gccgtcgctg
      121 agcctcgcgg gcgtcagggc gccttgcgga tgcccagctt gcgcatgcgc ccgcgcagcg
      181 tgctcgggtg catgccgggt gtgagcgcgg cgccgcgctc gccttcgatc acccagcggg
      241 tgcgtcccag cacgcgggcg ctcgacacgg cgctgaagca gttcctgcaa caggccggcc
      301 gcgccccagg ccggaagcag ccggaggtgc atcgaggatt agcctcccaa cagcactgcc
      361 gccccgtcct gcaaggggag atcggttcaa ctccgctcgc cgcgggattc ccgccgcgcg
      421 atccacagcg tggcggcgac gatcacgcag ccgccggcca gcgtcgagcg gctcggcgca
      481 tccgcgaaca ccagccagcc cagcagcgag gcccagacca gatccaggaa cttgacggac
      541 tgcgtggccg agatgtcggc cacgccgaac gagcgggcca ggcagtaatg gccggcgctg
      601 cccaggacac cgcagcccag gaaaccaagc cattgccagg ccgtgggcgc ctgccatccc
      661 ggcaaagcca gcggcaggct gaacagcgtc accgtgatcg actgccagag gacgatgacc
      721 tcgggtcgct catagcgcgt cagccccttg gtgatcagga acgaggccgc gaacaccggt
      781 atcgacgcca gcatcaggag cgtgtagacg cctgccgcgc cggacagctt gggagccacg
      841 acgatcagca cgccggcaag accgatcgcg gcggcgagcc agcgctccca gcgcatgcgc
      901 tcgccgaatg cccaggccgc gccgatcatg atgaagatcg gcccggtgaa gccgatggcc
      961 gtggtatcgg ccagtgtgat gtgcggaatt gccgtgaacc agatggtcag gcccaaggtg
     1021 tgcacggcgc cacgcaggaa ctgcccgccg atgttgcgcg gccggtacgc ggccaggccg
     1081 ctgcgcgcca ccagcggcag catcaccagc agcccgaaga cataacgaag gaactgcgtc
     1141 tggaacggat cgagctgcag cgtcaggctg cgcatcaggg tattgagcac gacgaacagc
     1201 agacccgcgg ccatggacca gagcaggccg cgaacgacgg ggtcgagcct tgccacgcgc
     1261 ctttgcgcga acgccgcatg ccgcatcagc acatggcgct tcacgcgccc gccaccgctc
     1321 tcaactgcca cggcatgtct ccatcgcgac cgaatatacg cgggcgatcg ggtcttgccg
     1381 tcgccgcagc gactgttcga tacgaaggtc atagccccgg tgccggcagg ctcttgcccg
     1441 ccgttccata tcggtgccgt cttcagggtg ggggtgaaat gctgcctacc gccgggcgtc
     1501 ggactggtcc tcggccaccg attgggtcgg cgcctcggag ccggcctcgc tttccggttg
     1561 gtcgtattcg gcgtgcctga cgatcgtgac cggcaccgga gacgcgtgga caagcgcgtg
     1621 cgaaaccgaa cccagcaggg cgctggcgat cgcgcccttg ccatgggcgc cgatgacgat
     1681 ggccgagcag ccgtactcct cggccaggtc gcacaagacg gagaccgggt cgccggaaac
     1741 gacttcgcat tcgtacgcca ctccggccgc gcggcacagc gcctcggccg gttccagcag
     1801 atgagcacca gccccggcgc tcacgtcctt gatcacgtcc gggtcgtgag ccacgacgat
     1861 ctcgtacaag gaggccggtg cctggacatt ggccagcacc aggtcggccc gcaggccctg
     1921 cccgatcagg cccagcgcat gacggacggc ctcgagcgac aggggcgaac cgtcaaccgg
     1981 gatcaggatt ttcatcgcag cctccgttcg gcactatgca ggtgcccacc ttagcaccgg
     2041 cccggacgcc gtgcaacggc acgcgtcaag cccggcgctg aaccgcccga tcgggttgcc
     2101 gccatggcgg ctgcggcttg aaccgcgcct gcaggaacgc caggaaaagc ttcacccgcc
     2161 cggccattcc ttcgcgccgg gccaccatgg ccatgatgtc cgcgtcgggc acgtgccaat
     2221 cctggagcac gcgcaccagc cggccttcgg ccaggcactc ggccacgttc cactccgatc
     2281 gcatgaccag gccctttccc tgcagcgccc attgccggac cacgtcgccg tcgttgctgc
     2341 taaggatcgc cggcacgcgc accgccagct cgctgcgttt cttgcggaag cgccacaggg
     2401 tcacgtcttc atcgttctcg cgcagcacga tgcaatcgtg cgtcgccaga tcttggggcg
     2461 cgagcggcgc cggacgatgg gccaggtacc cgggcgaggc gcagatgaaa cgggcgttgg
     2521 gcgcgatcgg gtaggcgacc acgctggaat cgggccgttc gccgatgtgg acgatcaggt
     2581 cgaaacggtt cgcgtcggcg accgaaacca catcgctgag cgtcagcgac accatgagct
     2641 tggggtgcag gctgtgaaaa tcggctatcg cgggggccag gtggtgccgc ccgaacccca
     2701 ggggcccgca gacatggagc gtgcccgcga cttcgcccga gcgcatgcgc aggtcgtcga
     2761 tcaggtcatc gacttgcgcc agcagctcga cggcccggct gtagaacagg gttccttcct
     2821 cggtcaggct gaaacgccgg gtgctgcggt ggatcaggtg cagcccgaga cgggtctcca
     2881 gctggcgcag gcgctgcgac acggccgagg gcgtcacgtc catgcgccgc gcggcctcgg
     2941 cgagcgagcc gctttcggtc accacgacga agaaccgcag gtcgacggca tcgttggaca
     3001 tgccctcatt gtccgccaac gccagacgcc ggcccattaa tactccacgc gcaggccgag
     3061 gccccgttcg ccggccaggc gcacggccag atcggccacg gccaggtcct gcaaggccac
     3121 acccgttccg tcgaagacgg tgatctcctg ggcggtacga cgtccatcgc acaggcccgc
     3181 gatgacggcg ccgaggctgc cgcgcaggct gcgttcggta atcaggccgg cctgatatgc
     3241 gtgctggcac tcaccgatgc tgaaggcctg cgccgccttg tccacgaaca aggcggcact
     3301 ggcaaccagt tcgggatcca gctcctgctt gcccttggta tcggcgccca tggcgttgat
     3361 gtgcgttccg ggacgcaccc acgacttttc gaccagcggc cgggtcgaag gagtcaccgt
     3421 gatcaggatg tcggcattgg ccgccacggc ctggcgatcc gcctgcggtg cgaattccag
     3481 gcccagctcg cggacctgcc ggccgaatgc gaccagtttg tcggccgacg ggttccatgc
     3541 atggacggtg cggatcgaac gcacggcaag cgtcgcctcc agctggtgga gcgcctgcac
     3601 accggtaccg atgatgccga gcaccgtgct gtcgggccgc gagaggtggc gggtggcgat
     3661 cgcgctggcc gctccagtgc gcacgcctgt gaggtagttg gcgctgacca gcgccgatgc
     3721 gcgtcccgtc cggggatcga acaacaaggt cgaggactgg tggttggtca atcccttggc
     3781 cgcgttatgg ggccagtagc cccccgcctt gagcccgagc acgggcgcct gggtgtcgca
     3841 gcctgtcttg acgccgaata cggcatcctg gtagccgacc gcctcgcgca cgaccgggta
     3901 attgcgcgcc tgcccgcgcg ccatggcggc gaagctgtgc tcgaccgccg cgatcgcgtc
     3961 gcgcatgccg accaacgcgc gtgcgtcctg ttccgtgatg accagcatgc cgtcctttcc
     4021 cccgtgtgat ttcaccagcc gctgaagcgc ggccaggtcg cggtgacctc gcggtccgcg
     4081 cccagcacct tgtagtggcc gtactgggcg gcggtggaac aggcatggtt gggcaggatg
     4141 cgtacctggg tccccaccgg aagatgcgcg gcacgatcgg gttgccccga gcggtgcgcc
     4201 atgatcccgt gctcctggtt ggcatcgacc aggatgaagt cgtcgagcgg tcggccgtcg
     4261 aggtcgcaga ccacgccata gccctggtcc accgcctgcc tggacgtgcc gcggtcgcgc
     4321 gacatggcca tccagccggc gtcgagcagg gtccagccct tctcgcgctg gtggccgatg
     4381 acggtcgcca gcaccgacag cgcgatgtcg tccacccggc acacgtccag gccggccatg
     4441 acgaggtcga aaaacacgaa aacgccggcc cgcacctcgg tcacgccctc gaggtgctcg
     4501 gcgaacaagg cggtgggggt cgagccgacg ctgaccaccg gggacgcgat acccgccgcg
     4561 cgcaggcgct gtgccgcatg aacggcagcc gcgcgttcgc gttcggccat ttccccgatg
     4621 gcatgcacgg acgggctttc gtaggaagcg cccgcatggg tgagcacgcc gcgcatcgac
     4681 acgccggcac gctcgaggta acacccgatt tccaccagct cgggcgcatc gggcttgaca
     4741 cccgagcggt ggccgtccga atcgatctcg atcagcactt cgaagccccg ctttgcctga
     4801 tgggcccgct ccgccaccag acgggcggtc tccatgcagt cgaggatcag cgtcagcctg
     4861 actcccctgt cctgcaacgc caggacgtgg tccagcttgt tcggcgcgat gcaaacggcg
     4921 tacaggatgt ccttgacgcc atgcgaggcg aaatactcgg cttccagcag cgtggagacg
     4981 gtgatcggcc cctggggcgt ctccatcagg cgccgcgcca catcgatgct cttgctggtc
     5041 ttgacgtgcg ggcgaaacgt gacgccgagc ccgcgcagac ggctgcgcat gcgcttgatg
     5101 ttgcgttcca tgcgggcttc gtccagcagc agtgccggcg tttcgagctg ctcgaatgat
     5161 tgctgtgcca tcttcaagct ccgccttcca gggcttcgcg cgccagcacc cgcccggggt
     5221 gggcgccggt ggacgcgcca tcgcgccaca ccaccgcgcc gttgacgatg acggtgtcga
     5281 tgccgcgcga ctgcgcgatg ggcacggcat aggtggcggc ttcgtccacc gtgtccgcat
     5341 cgaacagggt caagtcggcg taggcgccct cgcgcaggac gccccggtcc ttcaggccga
     5401 aattgcgcgc cgtgagcccg gtcatcttgt acacggcggt ttccagcgaa aacaggccga
     5461 gcaggcggcc gtagtggccc agcacccgcg ggaagctccc ccacagccgg gggtgcggcg
     5521 aggcgtcgtg cggcaggccg tcggagccga tcatggtctg gtcgaacttc agaatgcgct
     5581 gcacgtcggt ctcgtccatg cggaagtaga tcgcgccggc aggcaccagg cgcgcgatcg
     5641 cttcctcctg ggagacaccc atcttccgcg cgatgtccgc gaggtcctgg cccgcatact
     5701 cgggcagagt tttcgaccag ctgacgatca cgcggccgga gcccgccgca cggtcggccg
     5761 acaggatggt ggagccggcg gtgtacgggt agcagtccag gcagatcggc tgctgcgaca
     5821 tgctcttgcg gatgaaagcg agcgtttcct cggaacggcc gtagttcgcc tcgcccacca
     5881 ccttgtggtg cgagatcacg accggcacgc cgacttcgcg gccgatgcga aaggtctctt
     5941 ccagcgagtc catcacccgg tcggcctcgt tgcgcatgtg ggtgcagtac aggccgttga
     6001 actccttgag cgggcggcag gtctcgatca cttcctcggt cggcgcggcg atcgccggct
     6061 cgtagaacaa gccggtggac acgccgatgg cgccggcttc aagcgcctcc tgcaccagcg
     6121 cgcgcatgcg gccgatctcc tgcgcgctgg ccggttgctc caggtcgtcc atggtcgcga
     6181 cccgcaaggt ggtgtgcccc accagcaagg cgcaattggt ggccgcgggg ttggcgcgca
     6241 agccttcgac ataggacgcg aaggtcggga aatggaacca ttccccctgg tcgtccagca
     6301 ggttcaacgg cggagtgacg ggctggggga tcgggcgcgg catgggggcc agcgagatgc
     6361 cgcaattgcc ggcgatcacc gtggtcacgc cctggctgac cttgggcgcc atgtcgcggt
     6421 tggacagcat ggcgcggtcg tcgtgggtat gggcgtcgat gaagccgggc gccgcgatgc
     6481 gtccggccag gtcgatttcc acgtcgccac gttgcgccgc gagatcgccg atgcgctcga
     6541 tgcggtcacc gcggacgccg atgtcgcccg cgaaccgggg cgcgccggtt ccgtcgacga
     6601 tgctcgcgtt acggatcaga aggtcaaaat tatcctgcat cggaggcctc ttgttggaag
     6661 ttgtttacgc cgcgaggcgg tgcgccacgc atgtgggcga gcatgttgag cagttcggga
     6721 atcatctttt cgccgttcga ggtcgcctgg cggctgccgg ttttccgttg atggacatgt
     6781 cggtattcct tcaatccctg taatcaggag cctcttggtc gagctttcgc cgcagcgcat
     6841 ccaggtgcag ccgcgcccag tgctcgcgct cacgcccgaa ctgctggacc gtcttgccag
     6901 cgatgccgtc gggcacccgc cgcaagggcc gattgcagct catggccttg acctgcaact
     6961 cggccgcgcg ctccaggtaa tagaggtgat cgaaagcctg ggccaccgag ggcccgacca
     7021 cgatcacgcc atggtgggcc aggaacagca catgcttgcc gtccatcttg cgggcaatgc
     7081 gtgcgccttc gtcctggtcg tgcgccaggc cgttgtagct gtcgtcgtag gcgatgtcgc
     7141 cgtagaaccg caacgcctcc tgggacgccg gctccagccg cccgccttcc agcatggtga
     7201 tcgcggtcgc atagggcatg tgcgtgtgca gcacacagcg cgcctcggga tggatggcat
     7261 ggatctgcga gtggatattg aaggcggcga acccgccatc cctcgcgccc tgccgatcca
     7321 cgccgttctc gtcgatcagc atcagctcgc ttgcccgtgc ttccgagaag tacatgcccc
     7381 aggggttcag caggaacagc agcggctcgt cgttcaccct caagctgaag tgactgcaga
     7441 cgccggcctg tccgctgaac atggccgctc cccggaagac ggccgccagg tccttgcgtt
     7501 gttcggcgaa attcctgtcc acgtgggttt ccatttcagc tagcacgatc tatttgaaaa
     7561 gcgacggcag cccggacgac agcgccggca ccagcgtcag caggaagaca actccgagct
     7621 ccgccagcag gaaaggcatc actgcgcgca cgaccgcccc caccgtggaa ttggcaatgc
     7681 ggcaggcaag gaacaggcac actcctaccg gcggcgtgat caaccccagc gtgagcgtca
     7741 ggatcacgac catggcgaac tgcatcggat cgatgcccat cgcctggctc agcggcagca
     7801 gcatcggcat cagcaggacg atggccgcgc cgggttcgag gaacgtgccg accaccagca
     7861 gcaccagcgc gagcgccatc atgaacaagg tgggctggcc cttgaagaaa gttcccagtt
     7921 cctgcaccca gtcggcgatg ccgccgatgg tcaggacgta ggaaacgatc gtcgccgcgg
     7981 cgatgatgag gtagacggtc gcggtcatgc gggcgctcac ctgcaacgcg tcccacaact
     8041 ggggccagtc gatgctcctg aacacccccc aggtgatgat caacgcatag accaccgcga
     8101 caccaccggc ttcggtcgcg gtgaaggcgc cggagagcgt gccgaccacg atgatggcgg
     8161 gcagcgcgcc caccagcaag ccgtcgcgca cgagcttcag caattcgccc ttttcgacct
     8221 tttcgcgcgg ggagcgtggc agccgccctc cgcgggcctg gatgaagatc accagccagc
     8281 atgcgccggc cagcatcagg ccgggcacga tgccggcgac gaacagatcg atgaccgaca
     8341 cgcgcgtcac ggccgcatag atgatcatga tgacggacgg cggaatgatc gggccgatga
     8401 tggacgaggc gatcgtcacc gccgcggcgt agttcttgtc gtagccttcc tgcggcatgc
     8461 ccttgatgaa gacctggccg agtgcggcgg tgtccgccac ggccgcgccc gagatgccgg
     8521 caaagaacac cgacgacgtc acgttggtgt aggcggtgcc gccgtgcacg ccgcgcgtaa
     8581 tcaggccggc cagccggatc agccgcgtcg tgatgccgcc gcggttcatg atctcgccgg
     8641 cgaaaatgta gaacggcgcc gccagcagga tgaaactgtc catgccggtg aaaaggcgcg
     8701 tcggcacgga gatcaggtcc acccccacgg cccacatgcc gatcgcgcct gagaggccca
     8761 gcgcgaacag cacgggcacc ccgacgagaa gcagcgccac gaaagagatt cccaacgcga
     8821 tcattcgccc tcgcccttct tcatctggtt ggcgtaagcc aactcgggca gcgccatcag
     8881 gcagaccagc ttgactagca tcagggcagc ccccacgggg atggccaggt agggaatcac
     8941 catcgggatt tcaactgccg gcgaacgttg ctcgatgttc tggatcgcgg cgcggaagcc
     9001 ttcccacatc aagagcaggc agaacagcgc gatgcaggac agcaccagca agcggatcgc
     9061 attgcgcacg cggtcggagc gaaccttgtc gaaaatgctg atttccatgt gttcaccgcg
     9121 gaaaagggcc gagctgcccg ccaggagcac catccaaaca gtcgaatagc gcatggtttc
     9181 ctcgctccac gaaagcgggt cgatcaggac gtagcgaaaa atgatttgcg ccacgttcat
     9241 ggccaccacg gcaatgagca ggagtgccgc gatccggtct gcccagttgg aaagcgcaag
     9301 gccaacccgg aacgcctgcg tctggatgcg cagctgggca gccggacgcg cttccacctg
     9361 ggacaggacg gcactcatat ccgttttctc atcggccgta gagcgacggc tcatgcgcct
     9421 tggcggttgc gagcaggccc ttcacgaggt cggccccgac ttgctggcgc aggtactcga
     9481 gcaccggctc ctgggataca gcggcgaact gggccttttc atccatggtg gtcatgtgga
     9541 ccttgaggcc cttgcccttg atcaggtcga tgtacttctt ggcgcccgcc agcttctggg
     9601 tgttctcgat ccgggccgcc agcgtcgcgg cctcgagcac cacggccttc tcgtccgacg
     9661 tcagaccgtt gaagaactgg tcgttcatga tgaatgcgtg cacgcccaag atgtgctcgt
     9721 cggttgacat gtgtttctgc acgtcggcca ggttgtagtc gtagatcacc tgcgccgggt
     9781 tttcctggcc gtcgatcacg ccggatttgg cggccatgat gatttcagaa ccgggcatcg
     9841 gcgtggcggc agcgcccatg gactgcacga atcgcatgaa caccgggctt tccatcacgc
     9901 gcatcttcag ccccttcagg tccgccggcg acttgatcgg ccggacattg ttggtgaagt
     9961 tgcggaaccc gttctcgccc caggccatga cgcgaatgcc ggtttgcttg cgcatgtttt
    10021 ccgcgaggtc ggccatgaat gggctctcga acactttcca tgcatggttc gaagacttga
    10081 agaggtaggg aaccgacagg acctggatgt tcttgtagaa gccgccgagc gcgccggtat
    10141 cgaccaggca ggcctctacc gcgccttgct tgacctgctc ggcacaggcc cgctccgcgc
    10201 ccagctgggc gctcgggaag atcttgactt cgatctttcc ttcggtcttg tactcgacat
    10261 gatccttgaa cgccaccagc gcgttccact cggggttgtc ccccttgggc gcgttcatgc
    10321 cgaagcggat ggttttcgcg taccccgctg tcgacagccc cagtgccgcc gcagcggcga
    10381 cgagtaccag cacctgcttg aaatacttac tcatgaacta tcctttcgat caacaaaaaa
    10441 aaccccgggg ctgcgccccc ctgccaacga aactcccgaa cccggcccac gtcccgtcgc
    10501 ggcgcggtac cggcacactc acagcagtgc gccgcgtgcg gcgatgggcc agatggcctc
    10561 gactgtgtcg ccacgcacgc agaccagctc gtcataaagg ttgacggtcg ggtcgcaatg
    10621 gccgggaacc agcatcagcg actcgccgag cgacaccgcg gcgccaccgg cggccaacag
    10681 caccccgtgc tcgtcggcgg ccttgacgaa gctcaggtcg ggccgtcgcc acacggacgg
    10741 catgcccgag tcgacgctgg aggccttgag gccggcatcg accacggccc ggttcatggc
    10801 gggccggctc atcacggtag tacgcacgaa caaggcctgc tcgaaggcaa tgtcgccggc
    10861 tcccgcctgg ttgtcgccat agtcgcggtc catgaagaca taggagccgg cctggatttc
    10921 gttgaagatg cccgaatcgc gctcgtgcac gaagctgccg gtgccggcgc cggtcacgcg
    10981 ctcgacccgg atgccgcagg cctggatcgc gtcgcgcgtc gttcgcgcag cttcgaccgc
    11041 gttggcgatc gcgtcggcgc ggccgcgcgg ggaacgcaca tgctgggccg gcccgtggta
    11101 gcactgcagg cccgcgaagc gcagcggcgc gctggatgcc acgtggcgcg ccaacgccac
    11161 cgcggcctcg ccgggcgcca cgccgcagcg gtgcgcaccc acgtcgacct cgacatagac
    11221 gtccagcgtc acctcctgga cccgcgccgc ggcggcaagt gcctgcacct gctggggatg
    11281 gtcgaccagc acgccgatac gcgcggttcg ggccagcgcc gtcaggcgac gcagcttggc
    11341 atcgcccacc acttcgttgg tgaccagcac atcctggatg ccggcatcca cgaagacggc
    11401 cgcctcgctg accttctggc agcagatgcc gaccgcgccg agcgcgatct ggcgccgcgc
    11461 gatctcgggg catttgtggc tcttggcgtg cgggcgcagg cgtacgcccg tgccgttcag
    11521 cgcgctcgcc atgcgctgca tatttcgctc gaacgcgtcg agatcgagca ccagcgcggg
    11581 cgtatcgacc gcactgagcc ggtcgccggg ctgggcggcg ctccagggac tcatgatgca
    11641 atgctcgaca gctgcgcggc ctagtcgatc ctggcgcccg agtacttgac gacctgggcc
    11701 cacttcaccg tctcgcggcg tgacagctcc cggaactcct ccggcgtgct gggcgcggtt
    11761 tcggcgccca gcgccttgaa tcgctcgcgc acggccgggc tcgccaatgc ggcgcggacc
    11821 tccgcgttca ggcggctgac aatttccttg ggcatgtggg ccggccccac gatgccgccc
    11881 caggcgacca cctcgaaatc ggggacgccc gattcagcga tggtgggaat gtcggggtag
    11941 gcggtcgaac gctgcctgga cgaaatgccc agcgccttga cccgtccggc ctgcacgtgc
    12001 gggcccgata ccggcgtgtt gaccatcatg agctggatgc cgccgccgat caggtcggtg
    12061 atggccgacg acaccgcacg gtacggcacg tgcagcatgt gggtgcccgt cacggacttg
    12121 aagagttcga cgcccaggtg cgcggtggtg ccgtttccgt ccgaggcgaa tgcgatcttt
    12181 cccgggttct tcctggcata ggcgaccagt tcgcccacat tcttcaccgg caagtccttg
    12241 ttgacgatca tcaggttgac gacccgcagg gtgttggaca ccagcgtcag gtccttgtcc
    12301 acgtcgtaag gcacgttgct catcagtgaa cggttggtcg cgagcgaaac gacgttgccg
    12361 taagccagcg tgtagccgtc cgccggcgcg cgcaccagtt ccatggtgcc gatcacatag
    12421 gagccgccgg gcttgttgtc gatcacgatc ggaacgccca tctgggtgga cagttgcgcg
    12481 gccagactgc gcatcaggac gtccggggca ccgcccggcg ccgacggcac gatcaggcgg
    12541 atcggcttgt cggggtaggc cgcgaagccg ggcagcgaca cggcggccag cacggcaaag
    12601 gcaacggtga gcagcaggga gcgtgggcgc atggcgggcc ttggggatgg ttgaacggca
    12661 accgggaggt tggacagacg ctcgattcta gaaaccctat tcggaaaaac aactaaccga
    12721 gcctacatgc acggttaagt tgttctaacg aaagggcgcg cgcgctgccc aacacggcga
    12781 aacgtcttcg tgcgacgcgg gcacggcgtt ttttgatgaa gatcaagtca gggcgcgttt
    12841 gacagcccat ggaacatggc ttcggctaaa caatagtgaa caagtttccc ttgtagccag
    12901 aaatgccatc aacctcgacc gatcttttcc aggagtggcg cgtcgcgcac cgcgcggcca
    12961 ctgcggctga aaaggccatg ttgaacgcat cgatccgcgc gctcgagggc aagggcgagc
    13021 cgccctccca ggccgaggcg caggcaatca ggcgcctgcg cgcggttgcg gatgatctgt
    13081 tccagcaggc gatggcccag atgggggaga tggcggtcat ggcaagaacc gccggcaacg
    13141 gagtgtcgcg caccaccggg ctcacgccgg acaagctcaa aggcacccaa ccctagcgag
    13201 aaacccgggc cggggcatcg ctatcatggc gcctttggag catgagaagc ggttgattcc
    13261 cgaccgcaag ccgccgatcg caaatgagca aagcccagct gacggggtac gtcgagccca
    13321 acgaggaaac gcccctggcg acacgggtgg aagacgttcg catccgcgac gtgcggccgc
    13381 tgatttctcc cgcattgctc cagtacgaac tgccggtgag cgaacccgcg cagctcttcg
    13441 tggaggacag ccggcgcaag atcgccgccc tccttcgcgg cgaagatccg cgcctgctgg
    13501 tggtggtcgg cccctgttcc atccacgaca gccagcaggc actcgaatac gcccgtcgcc
    13561 tgcatgcgct ttccgggcag ctgaagcaag acctgctgct ggtcatgcgc gtttacttcg
    13621 agaagccgcg cagcaccgtg ggctggaagg gcttcatcaa cgacccgcgc ctggatggca
    13681 gcttccacat caacgaaggt ttgcggcgag cccgcgagct gctgctgtcg attgcgcaga
    13741 tgggcttgcc ggccgcgacc gaatttctcg acctgttgag cccccagtac atcgtcgacc
    13801 tgatcagctg gggcgcaatc ggggcccgca cgaccgagag cccgagccac cggcaactcg
    13861 cttccgggct gagctgcccg gtcgggttca agaacggaac ggatggcggc gtgcagatgg
    13921 cggccgatgc cctggtcgcc tgccgctcgc ctcatgcttt catgggcatg accaagatgg
    13981 gccaggtggc aattttcgag accaccggca acggcgattg ccacatcatc ctgcgcggcg
    14041 gctcgcgcgg cccgaactat tcgccggccg acatcgatgc cgcctgtgcg accctgcgca
    14101 agtcgggcgt ttccgagcgg gtcatggtcg actgttcgca cgcgaactcc gcgaagaagt
    14161 acgcacggca aatcacagtc gcgcaggaac tggccgcaca gatcgcggac ggtgagcacc
    14221 gcatcctcgg cttgatgatc gagagtcacc tcaaacccgg tcgccaggac ctgcgcgtgg
    14281 gcgtgcccct ggagcccggc gtttccatca ccgatggttg catcggctgg gagcagaccg
    14341 agactgtgtt gcggcaactc gccgaggcca cgcgccagcg ccgggcgcgg gccggctagc
    14401 cgaatctcga cagcggccga gcaaataaac actgctacgg aagagttgaa agagccgtcg
    14461 ggtcttgaat cccgccgaaa agcccttatg attagcactc gctggtgatg agtgctaaca
    14521 gcacttcact cctccgcaag ttaatgagcg ctaacttagt tggcgctttt ttgtccccct
    14581 cgtttcagtc aaggagatgc aatgaaactt cgccccctgc acgatcgcgt gatcgtcaag
    14641 cgtatcgaca gtgaaaccaa aaccgcctcg ggcatcgtca tccccgacgc tgccgccgaa
    14701 aagcccgacc agggtgaagt gctggccgtc ggccccggcc gcaagaccga caagggcgaa
    14761 ctcattgccc ccaacgtcgc cgtcggcgac cgcgtcctgt tcggcaagta cagcggccag
    14821 accgtcaagg tcgaaggcga cgagttgctg gtcatgaagg aagacgacct gttcgccgtg
    14881 atcgaaggcg cccccgccgc cggcctgaag aaggtcgcct gattcttgcg aagccgcgat
    14941 ccattccaac caatttttga ggtaacagca tggcagcaaa agacgtagtt ttcg
//
LOCUS       DASWIY010000163        18523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1576610, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000163 DASWIY010000000
VERSION     DASWIY010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576610"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VGA59_06290"
     CDS             complement(<1..761)
                     /locus_tag="VGA59_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9719324.1"
                     /translation="MAYALELKDLRKSFGKTEIIRGASLAVNAGERVAVIGPNGAGKS
                     TLFNLISGRFAPTSGEVLLNGHRIDGKRPFEINRLGLSRSFQITNIFPKLSVFENLRC
                     GVLWSLGYRYTFLKFLADLHDANDRAEQLLDMIKLHRKRDVLAINLTYAEQRALEIGI
                     TIAGGANVILLDEPTAGMSKSETRRFIELIKEVTVGKTLLTVEHDMGVVFGLADRIAV
                     VVYGEVIAFDRPEAVRANARVQEAYLGSHVADAQV"
     gene            complement(761..2137)
                     /locus_tag="VGA59_06295"
     CDS             complement(761..2137)
                     /locus_tag="VGA59_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9719325.1"
                     /translation="MSVVPQQLEQTAAAEATGAVAASATRYYQFKPYNVGRWIIWSLF
                     ALVLLVAPLVFTSSLSQTMLSQMGIAIIVCLSYNMLLGQGGMLSFGHAVYSGLGSFLA
                     IHTLNFVSDGSLPLPVSLIPIVGGLAGMTFAILLGWVTTKKAATPFAMITLGIGELVW
                     AMSLMFPEFFGGEGGISGNRVTGARAGGIANALGLTFGPQIQLYYLIAFYTFVCTALM
                     FAFTRTPLGRMLNAVRDNPERVEFVGYDTQKVRYIAFIIAAFFAGISGGLAALNFEIV
                     TSEVVSGYRSGAYLLFTFLGGATFFFGPIIGAILMVLAFVLLSEFTKAWLLYLGLVFL
                     FMVMYAPGGIASLIMMNLRVAAFGRLRDLWVSYLALAATALIVLVGAGAMIEMVYHLK
                     LNEALGPELRFLGTTLNARGVNSWFGSAFVMLTGLGLFELTRRHFLHQWGEIQEFIEK
                     EMKRREAL"
     gene            complement(2141..3088)
                     /locus_tag="VGA59_06300"
     CDS             complement(2141..3088)
                     /locus_tag="VGA59_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9719326.1"
                     /translation="MEFFTISLLNGISYGLLLFMLSSGLTLIFSMMGVLNFAHASFYM
                     LGAYFAFTTTQLIGYWPALVVAPMIVALLGAAFEKYALRRVHKFGHVPELLITFGLSF
                     IVLEMVQLIWGTSSVDYRVPDALEGPLFTLYGTQFPTYRAFMMLVAVLMLIALWLVLT
                     RTRIGLVIQAALTHPDTVEALGHNVPRVFMMVFGGGAALAGLAGVIGGNAFVTEPGMA
                     AAVGPVIFVVVVVGGMGSLSGAFVASLLIGVIQTFAVGMDNSLLGAASSVGWNITPET
                     FGYALWKLRISQVAPILPYMFLVLMLIFRPKGLLGTREG"
     gene            complement(3228..4493)
                     /locus_tag="VGA59_06305"
     CDS             complement(3228..4493)
                     /locus_tag="VGA59_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9719327.1"
                     /translation="MKFAAKCVAIGAILACAGPLFAQQAAPAAVKGQTVRIAFMDPLS
                     GPFANVGQNQLKSWQFVADRFSGAKNPAGVKFEVVGFDNKGSPQESLNTLKAAIDQGF
                     RYVTQGNGSGAATAIMEAVSKHNERNPGKEVLYLNYAAVDPALTNEKCNFWHFRLDAD
                     TSMKMEALTSFMKDQPKVSKVYLINQNYAHGQQVNKYFKDAIPRKRPDVKIVGEDLHP
                     IGQVKDFSPYVAKIKQSGADTIVTGNWGTDLTLLVKALNDSGLKIPMYTYYSGVTGTP
                     TALAAGGEGEVYVVAYGHSNHPGELGQMGAEFKKKFNDDYYTFATYNGIHLLAGAMAK
                     ARSTDPLKVARALEGLQVKSFAGDVTMRASDHQLQQSMYITKWQKAKKGEYNVENTGY
                     TFVPIKQMDPYVSSTPTSCQMKRPGAAAS"
     gene            complement(4662..6305)
                     /locus_tag="VGA59_06310"
     CDS             complement(4662..6305)
                     /locus_tag="VGA59_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HEX9719328.1"
                     /translation="MLGLMQSQPLLISSLIDFAERHHGDVEIVSRRVEGDIHRYSWRQ
                     VASRARQVAQALDGMDLLFSDRVATLAWNGYRHLEIYFGVSGSGRVLHTINPRLHPDQ
                     IAWVANHAEDQVLCFDMTFLPLVQAVHARCPNIRQYVALCDSDKLPEGSGIPNLVSYE
                     AWIGARSTDYQWPSFDENSASSMCYTSGTTGNPKAALYSHRSTILHAYAAALPDVMCL
                     SARDSVLPVVPMFHVNAWGIPYSAALVGAKLVFPGPALDGKSVYELIEAEKVNYAAGV
                     PTVWQMLLGHMKPNKLRFSTLKRTVIGGSACPPAMIDAFREDYGVEVLHAWGMTEMSP
                     LGTLCTLKNKHLSLPAGEQMKIRLKQGRAIFGVDMKIVGDDGKELPWDGKTPGDLYVK
                     GPWVVREYFKNEGGDPLVPDERGVGWFPTGDVATIDPDGYMQITDRSKDVIKSGGEWI
                     STIDIENIAVAHPAVAMAACIGMKHPKWDERPVVAVVKKPGAEVTKEELLKFYEGKTA
                     KWQVPDDIVFVDTIPIGATGKMLKTKLREMLKDYKLPTA"
     gene            6530..7225
                     /locus_tag="VGA59_06315"
     CDS             6530..7225
                     /locus_tag="VGA59_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9719329.1"
                     /translation="MFHDISRPSTQFLQMQPWFAKLPLETRESIADRVFTQQGRKGDV
                     LLRSGDEVKGWYAVLSGLVKLQSQSPQGRLSVFLGVPGGEWFGEGSAMKTEPRRYDVI
                     ALRDSELLCLPRAQFDLLRSASLEFNQALLLQMNMRLSQAMSVIEAGRIRSPEQRVAL
                     YLSRLFWHGMRKLKLSQEELGNLVGLSRQTVNRVLNALEQQGLVSLEFGRIGILDEDA
                     LVALVFGQPPDKP"
     gene            complement(7222..8385)
                     /locus_tag="VGA59_06320"
     CDS             complement(7222..8385)
                     /locus_tag="VGA59_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX9719330.1"
                     /translation="MLAASPTDDDAGAVLFVDDEPTSRKWFARTFRDEFDVVTAAGVG
                     EALEVLGERGDRFAVLVTDYRMPERDGMKLLQAVQRDYRHLVRVLATAYAGKELAIAA
                     VNEGKVLRILEKPLDDALTRDALREAVALHRAQAFERAMNDGRAAAMRETLGFLAHEL
                     NTPLATVRGCVSAVAARYQTPAPGAPAGMVQFTESYPGEVMAALERAERRALYSQSLV
                     STFVQSARDAYPGAPAQTVSASSLVAALLDEFPFEENERSCVSSDVLEDFTLPGRRDL
                     LYLVICTLAKNALLALRGTAGPTLRIAVGCDQTSSGPGRRWIRFSDNGPGIAPDVLAR
                     LTHEPVTTRAQSGGNGMGLMFCQRVMRSIGGVIEIQSSLGQGTTVSLNFATAS"
     gene            complement(8397..10892)
                     /locus_tag="VGA59_06325"
     CDS             complement(8397..10892)
                     /locus_tag="VGA59_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719331.1"
                     /translation="MKQLFAHYKLYHQHGPQMLRYAGLVGAFGFPIFYVLRFTKSSPP
                     YDDSWIRLAATLVCVLLVLKDRWPQRLKPYYIAFSYPALIFSLPVFLVFTSLKNGGGA
                     AAVGNTLMVAFFVILMTDWRNTIVMLVVGISGGVLLYLAVDPNPSIPMEYVARLPLLV
                     LVVIGGSFFKFAGRQAAAEAMREGYASLAGSIAHEMRNPLSQIKHNLERMQQALPAPT
                     TSRQARPLALHEVDALYRHLAESELAVKRGLQVIAMTLDEVSEKPMDTAAFSYVSAAE
                     ATYKAAQEYGYETDAERSRVSVRVVEDFSFRGDETAYLFVLFNLIKNALYYVALCPQA
                     EVTITVERQQVKVRDTGPGIPASMLARLFEPFRSAGKSDGTGLGLAYCQRVMRGFGGQ
                     IGCESVEGQYAEFTLRFPPISEQEREAHRLSVLDRARMAFAGKRLLIVDDDSAQRMTT
                     RHKLQVLGVAIDEAANGQRALDMLSKQRYDLVLLDLNMPLLDGYALAEKIRQGQAPAN
                     RNVRIVAHTSEPAHLASVKTQKAGMDGFVGKPCAQLPLLEALLHALAHPAATVQPDMA
                     LLAGRRVLVADDSPFNRMAVAAYLQHVGATVVEAPHGPAVLAQLPTCDAWDAILMDIN
                     MPGMNGLETTHAIRSSGMAWRGVPIIALTAHSDEETIRAAHEAGMNDFITKPVDSAIL
                     YAKLIQLVSGEVVSPKPANAAALGAAANDLDVELLNVERLEGFRRIGILHELLNDYLP
                     EIALLVDRLEHSAGSRNMEESIDALHSLLGMSGEAGAQALHSLVRRSYVSMIEARAWP
                     ADSDWLAHIKSAAVRSEQALRAYGPMQSTVTAA"
     gene            complement(10898..11293)
                     /locus_tag="VGA59_06330"
     CDS             complement(10898..11293)
                     /locus_tag="VGA59_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX9719332.1"
                     /translation="MPIGSGLTLTETLLAQPASPRVTMGKGSWEVGRLVLAPEYRSDP
                     DALKRCLFLSLSYLSRHTEAHDLYATCSHVLSRLYRRFGFTAFAKDVVLAGTEKSYTL
                     IQGYAAQVLDSLAGVKPDKARIVPAGASH"
     gene            complement(11308..11637)
                     /locus_tag="VGA59_06335"
     CDS             complement(11308..11637)
                     /locus_tag="VGA59_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719333.1"
                     /translation="MISSTGSSAGRSVAVGMSGHASQSASQHGLFFESGAAAAQPACA
                     ESHRLSVEEFGLRHLKTPDEIERILHLRKAIDLSVHSHALTNFLALEKKETSAVLSVP
                     SNCAGKS"
     gene            11844..13349
                     /locus_tag="VGA59_06340"
     CDS             11844..13349
                     /locus_tag="VGA59_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX9719334.1"
                     /translation="MNQVANAVDAPVRRLDLGGLAWRNSFAGLGPAFYTELEPIPLPS
                     PYLVGLNRPLAAELGLSARLLAGQEGIEAFTGNLPIAGSRPLASVYSGHQFGVWAGQL
                     GDGRAILLGEIETPAGPQELQLKGSGLTPYSRMGDGRAVLRSSIREFLGSEAMRGLGI
                     PTTRALCVTGSDAPVRREGIETAAVVTRVAPSFVRFGHFEHFSAREQYGELAKLADYV
                     IDSFYPECRASSRFSGNAYAALLEQVSERTAAMVAQWQAVGFCHGVMNTDNMSILGLT
                     IDYGPFQFLDAFDPGHICNHSDEQGRYAYNKQPNIAYWNLFCLGQALLPLIGDQELAL
                     AALESYKAVFPRELEARMRAKLGLSDARAADRELIEGILKLLAADRVDYTIFWRRLSR
                     HAAGDAADTVRDLFLQRDAFDAWLIQYSDRLDPVGRIEAADLMLKTNPKYVLRNHLGE
                     LAIRQAKLKDYSEVEDLLALVQAPYDEHAGHDEKAGFPPDWASQIEISCSS"
     gene            13346..13762
                     /gene="msrB"
                     /locus_tag="VGA59_06345"
     CDS             13346..13762
                     /gene="msrB"
                     /locus_tag="VGA59_06345"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX9719335.1"
                     /translation="MTYKIEKSDAEWKALLAQKGAEPGAFEVTRHAATERPFTGKYEA
                     VWDDGSYHCLCCGAKLFDSVAKFDAGCGWPSFSEALPGAIKEITDRSHGMTRSETVCA
                     QCGAHLGHVFPDGPSDTGLRYCMNSASLDFKPEQGK"
     gene            13771..14316
                     /locus_tag="VGA59_06350"
     CDS             13771..14316
                     /locus_tag="VGA59_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEX9719336.1"
                     /translation="MKLLIDFFPIILFFVAFKVAGIYIATGVAIVATIAQIVWLRHRH
                     GKVEPMQWVSLGVIVLFGGATIALQNETFIKWKPTVLYWLMAGTLAAGQLLFHKNLLK
                     TLMGSQMELPDAAWRITNWSWIVFFALMGVLNLWVAYNFETNTWVNFKLFGGLGLMAL
                     FVIGQALYLGRYLKTDQVPEP"
     gene            14313..14594
                     /locus_tag="VGA59_06355"
     CDS             14313..14594
                     /locus_tag="VGA59_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX9719337.1"
                     /translation="MSGTGVTAEAMRQRLSELLAPSMLEVIDESFQHEGHPGADGTGF
                     GTHFRVRIASPLFAGRSRVARHRLVYDALQVFVDRGVHALAIETSDPPS"
     gene            14612..15391
                     /locus_tag="VGA59_06360"
     CDS             14612..15391
                     /locus_tag="VGA59_06360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9719338.1"
                     /translation="MKQVFLAATAAALIGMAAPALAQNVAIVNGKPVPKARVDALAAQ
                     LAKSGRPISPEMQGQLKDEVIAREIFMQEAQKRGLEGSDDFKAQMELARQTLLIRELF
                     ADYQKSNPVTDADIKAEYDKFAAANGGKEYRARHILVEKEDEAKTIIASLKKGGKFDE
                     IAKKQSKDPGSGANGGDLDWANAASYVPEFSAALVKLNKGQTTEAPVKTQFGWHVIRL
                     VDIREAQLPKLEAVKPQIAQQLQQQKLAKFQEELRGKAKIE"
     gene            complement(15536..16288)
                     /locus_tag="VGA59_06365"
     CDS             complement(15536..16288)
                     /locus_tag="VGA59_06365"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HEX9719339.1"
                     /translation="MPPARLATIDTLRGIAIVWMTVFHFCFDLNYFGWLKQNFYTDPF
                     WTWQRTAIVSLFLFCAGLGQAFAFAQGQSWGRFCKRWLQIAGCALLVTAGSYWMFPKS
                     FIYFGVLHGIAVMLVIVRLTAGWGRWLWLAGAVALAVKPLALFAHVQWPALDFLNHMT
                     WNWLGLISRKPVTEDYVPLFPWLGVMWWGVAAGQWFLARRKDLLQARLPAAAAPLAWL
                     GRWSLTWYMLHQPVMIGILAAARWLSLRAGLA"
     gene            16545..17006
                     /locus_tag="VGA59_06370"
     CDS             16545..17006
                     /locus_tag="VGA59_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719340.1"
                     /translation="MNKPARSAAELAAPVLDALNRGNVIEAFKLMRAASGLGLREAKQ
                     ALDAYRRGKQPSAAQPEFPRALPGGSLPPSVVEALQRGNKIEAVTRLRQQTGLGLKEA
                     KDAVELFRQLHPRPDDPSPGEVRDSGGALVWVAVALAVVFLLAYRIFQRLG"
     gene            17093..>18523
                     /locus_tag="VGA59_06375"
     CDS             17093..>18523
                     /locus_tag="VGA59_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX9719341.1"
                     /translation="MTEAPRPPSDGDWHLREAHEVARAHEVNPITGLQEHQVLERAER
                     HGRNELPVTGGRRSLWSLALDQFSDFMILVLIAAAVVSGLIGDLIDTLAILVIIVLNA
                     VIGVVQAWRADRALAALKQLAAAQATVVRGGQVRQVPAHNLVPGDVVLLEAGNQVPAD
                     LRLIEIAQLKVDESALTGESVTVAKHAGVLGGALHALGDRLNMAFKGTTATHGRGRGV
                     VVATGMSTELGKVAGLLGQEADQSTPLQRRLAAFGKRLAIAVLAICAVILGVGLLRGE
                     PLLLMVLTALSLAVAAIPEALPAVVTVLLALGARRMVAVNALVRRLPSVETLGSVTYI
                     CTDKTGTLTQNRMQVESLVVDGEAAPVTGTESEPARRVLLRALALCNDATPSADGGWQ
                     GDPTEVALAEAAAAAGLDKTALQSEWPRVSELPFDAERKRMTTVHGTLQGYVAYTKGA
                     PESVIPLCGDACPGGLDRAAWLQRAEA"
BASE COUNT         3356 a         6012 c         5964 g         3191 t
ORIGIN      
        1 tccacctggg cgtcggccac atgggaaccg aggtatgcct cctgcacgcg ggcattggcg
       61 cgcacggcct ccgggcggtc gaaggcgatc acctcgccgt agacgaccac ggcgatcctg
      121 tcggccaggc cgaagaccac gcccatgtcg tgctcgaccg tcagcagcgt cttgcccacg
      181 gtgacctctt tgattagctc gatgaagcgc cgggtctcgc tcttgctcat gccggccgtc
      241 ggttcgtcga gcaggatcac gttggcgccc ccggcgatgg tgatgccgat ctccagcgcc
      301 cgctgctcgg cgtacgtcaa attgattgcg agcacgtcgc gcttgcggtg cagcttgatc
      361 atgtccagca gttgttcggc gcggtcgttc gcatcatgca ggtcggcgag gaacttcagg
      421 aacgtgtacc ggtagcccag gctccagagc accccgcagc gcaggttctc gaagacgctc
      481 agcttgggga agatgttggt gatctggaag ctgcgcgaca gcccgagccg gttgatctcg
      541 aacggccttt tcccgtcgat acggtggccg ttgagcagca cttcgccgct ggtgggggcg
      601 aagcggccgc tgatcaggtt gaacagggtg gacttgcccg ctccgttcgg gccgatcacg
      661 gcgacccgtt cgcccgcgtt aacggccagg ctggcaccgc ggatgatctc ggtcttgccg
      721 aagctcttgc gcagatcttt cagttcaagg gcgtaagcca ctagagtgcc tccctgcgct
      781 tcatttcctt ctcgatgaat tcctggatct cgccccattg gtgcaggaaa tggcggcgcg
      841 tcagttcgaa caggccgagg cccgtgagca tgacgaacgc cgacccgaac cagctgttga
      901 cgccgcgcgc attcaaggta gtgcccagga agcgcagctc gggccccagg gcctcgttga
      961 gcttgaggtg gtagaccatc tcgatcatcg ccccggcacc gacgagcacg atcagcgcgg
     1021 tcgcggcgag cgccaggtaa ctgacccaga gatccctcaa ccggccgaac gccgccaccc
     1081 gcagattcat catgatcagg ctggcgatcc cgccgggggc atacatgacc atgaacagga
     1141 agaccaggcc caggtacagc agccaggcct tggtgaattc ggacagcagc acgaacgcaa
     1201 gcaccatgag gatggcgccg atgatggggc cgaagaagaa ggtggccccg cccaggaagg
     1261 tgaacagcag gtaggctccg gacctgtagc cgctgaccac ctcggacgtc acgatctcga
     1321 agttgagggc cgcgaggccg cccgaaatcc cggcaaagaa ggcggcaatg atgaacgcga
     1381 tatagcgcac cttctgcgtg tcgtagccca cgaactcgac ccgctcgggg ttgtcgcgca
     1441 cggcgttgag catgcgcccg agcggcgtgc gggtgaacgc gaacatcagc gcggtgcaca
     1501 cgaaggtgta aaaggcgatc aggtagtaca gctggatctg cgggccgaag gtcaggccca
     1561 gcgcgtttgc gataccgccc gctcgcgcgc cggtgacgcg attgcccgag atccctcctt
     1621 ccccgccgaa aaattccggg aacatcagcg acatggccca gaccagttcg ccgatgccca
     1681 gggtgatcat cgcgaagggc gtggccgcct tcttggtcgt gacccagccg aggaggatcg
     1741 cgaatgtcat gccggcaagc ccgcccacga tggggatgag gctcaccggc agcggcaagc
     1801 tgccatcgct gacgaagttg agtgtgtgga tggccaggaa cgagcccagg cccgagtaca
     1861 ccgcatggcc gaaactgagc atgccgccct gccccagcag catgttgtac gagaggcaga
     1921 cgatgatggc gatgcccatc tggctgagca tcgtctgcga caggctgctg gtgaaaacga
     1981 ggggagccac cagcagcacc agcgcgaaca ggctccagat gatccagcgg ccgacgttgt
     2041 agggcttgaa ctggtagtaa cgcgtcgccg atgcggcgac ggcgccggtt gcttccgccg
     2101 cggcggtctg ctcgagctgt tggggtacta cgctcacgtc ttatccctct ctggtgccaa
     2161 gaagtccctt gggccggaag atcagcatca gcaccaggaa catgtagggc aggatgggcg
     2221 ccacttggct gatcctgagc ttccacaacg catagccgaa cgtctcgggc gtgatgttcc
     2281 agcccaccga ggatgcggcg cccaggagcg agttgtccat gcccaccgcg aacgtctgga
     2341 tcacgccgat cagcagcgat gcgacgaaag cgccggagag cgaccccatg ccgcccacga
     2401 ccaccacgac gaagatcacc gggcccaccg cagcggccat tcccggttca gtgacgaacg
     2461 cgttgccgcc aatgaccccg gccaggccgg ccagcgcggc gcctccgccg aacaccatca
     2521 tgaacacgcg gggcacgtta tggcccagcg cctcgaccgt gtccgggtgg gtgagcgcgg
     2581 cctggatgac cagcccgatg cgggtgcgcg tcagcaccag ccacaaggcg atgagcatca
     2641 gcacggcaac cagcatcatg aacgcccggt acgtcggaaa ctgcgttccg tacagggtga
     2701 acagcggccc ctccagggcg tccggaacgc gatagtccac cgagctcgtg ccccagatca
     2761 gctgcaccat ctccagcacg atgaacgaca gcccgaacgt gatgaggagt tcaggcacat
     2821 ggccgaactt gtgcacccgg cgcagtgcgt atttctcgaa tgccgcaccg agcaaggcaa
     2881 cgatcatcgg tgccacgacc agcgccggcc agtacccgat cagctgggtc gtggtgaacg
     2941 cgaagtaggc tcccagcatg tagaagctgg cgtgcgcgaa gttcagcacg cccatcatgc
     3001 tgaagatcag ggtgaggccc gagctgagca tgaacaggag cagtccgtag ctgatgccgt
     3061 ttagcaggga gatggtgaaa aattccatgc gatctcaatg acgaatgacg aatgacctcg
     3121 cgctgcgcgc accgatgacg cgatcgcgcc gcatgacccg ctcattcgtc gttggagccg
     3181 aaggcgaagt catgcggcgc agccgcggtc attctggaaa caggctgtca ggatgcggcc
     3241 gccccgggcc tcttcatctg gcagctggtc ggcgtgctcg acacgtacgg atccatctgc
     3301 ttgatcggca cgaaggtgta gcccgtgttc tccacgttgt actcaccctt cttggccttc
     3361 tgccacttcg tgatgtacat ggattgctgc agctggtgat ccgaggcgcg catggtgacg
     3421 tcgccggcga agctcttgac ctgcaggcct tccagcgccc tggccacctt gagcggatcg
     3481 gtggaccggg ccttggccat ggcccccgcc agcagatgga tgccgttgta agtcgcgaag
     3541 gtgtagtagt cgtcgttgaa cttcttcttg aactcggcac ccatctgccc gagctcgccc
     3601 ggatggttcg agtgcccgta ggccacgacg tagacctcgc cctcgccgcc ggccgccagg
     3661 gccgtgggcg tgcccgtcac gccggaatag taggtgtaca tcgggatctt gaggccggag
     3721 tcgttaaggg ccttcaccag cagcgtcagg tcggtgcccc agttgccggt gacgatggtg
     3781 tcggcgccgg actgcttgat cttcgcgacg tagggcgaga agtccttgac ctggccgatc
     3841 gggtgcaggt cttcaccgac gatcttcaca tcggggcgct tgcgcgggat cgcgtccttg
     3901 aagtacttgt tgacctgctg gccatgagcg tagttctggt tgatcaggta gaccttgctg
     3961 accttgggct ggtccttcat gaaggacgta agcgcctcca tcttcatgct ggtgtcggca
     4021 tccaggcgga aatgccagaa attgcacttc tcgttggtga gcgccggatc caccgccgcg
     4081 tagttcaggt acagcacttc cttgccaggg ttgcgctcgt tgtgcttgga gaccgcctcc
     4141 atgatcgccg tggccgcgcc ggagccattg ccctgggtca catagcgaaa gccctgatcg
     4201 atcgcggcct tgagcgtgtt cagactttcc tggggcgagc ccttgttatc gaagccgacg
     4261 acttcgaact tgacgccggc cggattcttg gcgccggaga agcggtcggc gacgaactgc
     4321 cagctcttga gctggttctg gcccacgttg gcgaacggac ccgacagcgg atccatgaac
     4381 gcgatgcgga cggtttggcc cttgacggca gcgggagcgg cctgctgggc gaacagcgga
     4441 ccggcgcatg ccaggatcgc gccgatggca acacatttcg cggcaaattt catcttgatc
     4501 tccttatgaa tcgaactgcg cgcagcgtac cttttttgtg cactgcaacg ctcagggaat
     4561 gcccctaaga cagggcgttt ctatcgccga tgcgacacgc caggcatggt ccagctacgc
     4621 gagttctgca ctgcaagctg cggggccgcg ccgcgccggc atcaggccgt gggcaatttg
     4681 tagtccttca gcatctctcg caatttggtc ttcagcatct tcccggtcgc gccgatgggg
     4741 atggtgtcga cgaacacgat gtcatcgggc acctgccact tggcggtctt gccttcgtag
     4801 aacttgagca actcctcttt cgtcacctcg gcgccgggtt tcttgaccac ggcgaccacc
     4861 ggccgttcat cccacttcgg gtgcttcatg ccgatgcagg ccgccatcgc caccgccggg
     4921 tgcgccaccg cgatgttttc gatgtcgatc gtgctgatcc attcgccgcc cgacttgatc
     4981 acgtccttgc tgcggtcggt gatctgcata tagccgtcgg ggtcgatggt ggccacgtcg
     5041 ccggtgggga accagccgac gccacgctcg tccggcacca gaggatcgcc gccttcattc
     5101 ttgaagtact cgcgcaccac ccaaggtccc ttcacgtaca ggtcgcccgg cgtcttgccg
     5161 tcccagggca gttcctttcc atcgtcgccg acgatcttca tgtcgacgcc gaagatcgcg
     5221 cggccctgtt tcaggcggat cttcatctgc tcgccggcgg gcagtgacag gtgcttgttc
     5281 ttcaacgtgc acagcgtgcc gagcgggctc atctcggtca tgccccaggc gtgcagcact
     5341 tcgacgccgt aatcctcgcg gaaagcgtcg atcatggcgg gcgggcaggc ggagccgccg
     5401 atcaccgtgc gcttcaaggt cgagaaccgc agcttgttcg gtttcatgtg gcccagcagc
     5461 atctgccaga cggtgggcac accggccgcg tagttgacct tttccgcctc gatcagctca
     5521 tagaccgatt tgccgtccag cgcggggccc gggaacacca gcttggcgcc gaccagggcc
     5581 gccgaatacg gaatgcccca cgcattgacg tgaaacatcg ggaccaccgg cagcaccgaa
     5641 tcgcgcgccg acaggcacat gacatcgggc aacgccgcgg cgtaggcgtg caggatggtg
     5701 gagcggtggc tgtacagcgc cgccttcgga ttgcccgtgg tgccgctggt gtagcacatg
     5761 ctcgaggccg agttttcgtc gaagctcggc cattggtagt ccgtggaccg cgcgcctatc
     5821 cacgcctcgt aactcaccag gttcggaatg ccgctgccct cgggcagctt gtcgctgtcg
     5881 cacagcgcca cgtactggcg gatgttcggg caacgggcgt gcacggcctg caccagcggc
     5941 aggaaagtca tgtcgaagca cagcacctgg tcttcggcgt ggttggcgac ccaagcgatc
     6001 tggtcggggt gcaggcgcgg attgatggtg tggaggaccc gcccggaacc gctgacgcca
     6061 aagtagattt ccaggtgccg gtagccgttc caggccagag tcgcgacgcg gtcggaaaac
     6121 agcaggtcca tgccgtcgag ggcctgcgcc acctggcgcg cccggctcgc cacctggcgc
     6181 cagctgtagc ggtggatatc cccttcgacg cgtcgggaga cgatctccac gtcgccatgg
     6241 tggcgttccg cgaaatcgat cagggaggag atcaacaacg gttggctttg catcaaaccc
     6301 agcatgtgta ctccatgaga cattgttatg gggcccttat cgtagtgctt gcagatcggg
     6361 atgggtagcc ttgcgcttcc accagggcct gtcgggcgaa ctgcttgcca ggccgataat
     6421 ggagacagcg cgagtcaatt gggggagtct gggggcatgg cgacgccttc gcaacaggtg
     6481 tcgccgcgac gacagctcgc gcgccgctga ccggcgcaac accgaaacca tgttccacga
     6541 tatttccagg ccttccacac agttcctcca gatgcagccg tggttcgcca aactgccttt
     6601 ggagactcgg gaaagcatcg cggaccgggt gttcacgcaa caaggccgca agggcgatgt
     6661 cctgctgcga tccggcgacg aggtcaaggg ctggtacgcg gtgctgtcag gcctggtcaa
     6721 gctgcaaagc cagtcgccgc agggtcggct gtcggtcttc ctgggagttc cgggcggcga
     6781 atggttcggt gagggttcgg cgatgaagac cgagccgcgc cgctacgacg tcatcgcgct
     6841 gcgcgattcc gaactgctgt gcctgccgcg cgctcagttc gacctgttgc gcagcgccag
     6901 cctcgaattc aaccaggcat tgctgctgca gatgaacatg aggctgagcc aggccatgtc
     6961 ggtcatcgaa gcgggccgca tccgctcgcc ggagcagcgt gtcgcgctct atctcagccg
     7021 actgttctgg cacggcatgc gcaagctcaa gctctcgcag gaagagctgg gaaatctcgt
     7081 cgggctgtcc cgccagaccg tcaaccgtgt cctcaacgcc ctggagcagc aggggctggt
     7141 atccctggaa ttcgggcgga tcggcatcct cgacgaagac gctctggtgg cgcttgtctt
     7201 cgggcagccg cctgacaaac cctaggaagc agtggcgaag ttgagcgaaa cggtagtgcc
     7261 ctgccccaga ctcgactgga tttcgatcac gccgccgatc gaccgcatga cgcgctggca
     7321 gaacatcagg cccatgccgt tgccgcccga ctgcgcccgc gtggtgaccg gttcgtgggt
     7381 caacctggcc agcacgtcgg gtgcgattcc cggcccgttg tccgaaaaac gtatccaacg
     7441 ccgtcccggc ccggaggagg tttgatcgca gccgacagcg atgcgcaagg tgggccccgc
     7501 agtgccgcgc aaggccagca aagcattctt ggccaaggtg catatcacca ggtagagcag
     7561 gtcgcgccgg cccggcaggg tgaaatcttc cagcacgtcg ctgctgacgc aggagcgctc
     7621 attttcctcg aacggaaatt catcgagcaa tgcggccacc agactgctgg cgctgacggt
     7681 ctgcgccggc gcccccgggt aggcgtcgcg ggcggactgc acgaaggtcg acaccaatga
     7741 ctgggagtag agcgcgcgcc gctcggcgcg ttccagcgcc gccatcactt cgcccgggta
     7801 gctttccgtg aactgcacca tccccgcagg tgcgccgggc gccggcgtct ggtaccgggc
     7861 cgccaccgcg ctgacgcagc ctcggacagt cgccagcggc gtattgagct catgagccag
     7921 aaaacccagg gtttcgcgca tggccgccgc gcggccgtcg ttcatcgccc gttcgaacgc
     7981 ctgagcgcgg tgcaacgcaa ccgcctcgcg caaagcatcg cgcgtgagcg cgtcatcgag
     8041 cggtttttcg agaatgcgca ggaccttgcc ctcgttcacc gcggcgatcg caagctcttt
     8101 ccccgcgtac gccgtggcca gcacacgtac cagatgccgg tagtcgcgct gcacggcctg
     8161 cagcaatttc attccgtcac gctcgggcat gcggtagtcg gtgaccagca cggcgaaccg
     8221 gtcgccgcgc tcgcccaata cctccagcgc ttcgccgacc cccgccgccg tcacgacatc
     8281 gaactcgtcg cggaaggtgc gggcgaacca ctttcgcgag gtcggttcgt cgtccacgaa
     8341 gaggacagcg cccgcatcgt catcggttgg actggcagca agcatgctcg tgttcctcag
     8401 gccgcggtga ctgtggattg catcgggcca taagccctca acgcctgttc cgaccgcacg
     8461 gcggccgact tgatgtgcgc cagccaatcg ctgtccgccg gccaggcgcg cgcctcgatc
     8521 atggaaacat aagaccgtcg cacgagactg tgcagggctt gtgcgcccgc ttcaccgctc
     8581 atgcccagca gcgaatgcag ggcatcgatg ctttcctcca tgtttcgcga accggcgctg
     8641 tgctccagcc tgtcgaccag cagggcgatc tcgggcaggt agtcgttcag cagttcatgc
     8701 aggatgccga tgcgacgaaa accctcgagg cgctcgacgt tgagcagttc cacgtcgaga
     8761 tcgtttgccg cggcgcccaa tgcggcggcg ttcgccggct tcggggaaac gacctccccg
     8821 ctgaccagct ggatcagctt tgcgtaaagg atggccgagt cgaccggttt ggtgatgaag
     8881 tcgttcatcc cggcctcgtg cgccgccctg atcgtttcct catccgaatg ggcggtcagc
     8941 gcaatgatgg ggacgccccg ccacgccatc ccgctcgagc ggatggcgtg agttgtctcc
     9001 aggccgttca tgcccggcat gttgatgtcc atcaggatcg cgtcccaggc gtcgcaggtg
     9061 ggcaactgcg ccagcacggc cggtccgtgg ggcgcctcca ccaccgtcgc cccgacgtgc
     9121 tgcaggtagg cggcgaccgc catgcgattg aaggggctgt cgtcggcgac caggacgcgc
     9181 cggcccgcca gcagcgccat atcgggctgg accgtggccg cgggatgcgc gagtgcgtgc
     9241 agcaatgcct ccagcagcgg cagctgcgca cacggctttc ccacgaagcc gtccatcccg
     9301 gccttttgcg tcttgacgct ggccaggtgc gcgggctcgc tggtatgggc gacgatccgg
     9361 acattccggt tggcgggtgc ctggccctgc cggatctttt cggccagggc gtagccgtcg
     9421 agcaacggca tgttcaggtc cagcaggacc aggtcgtaac gctgtttgga caacatgtcc
     9481 agcgcccgct ggccgttcgc ggcttcgtcg atcgcaacgc caaggacctg cagcttgtgc
     9541 cgcgtggtca tgcgctgggc cgaatcgtcg tcgacgatca acaggcgctt gccggcgaac
     9601 gccatgcgcg cgcggtcgag caccgacaaa cgatgtgctt ccctttcctg ctcgctgatc
     9661 ggcgggaagc gcagcgtgaa ctccgcgtac tggccctcca cggactcgca gccgatctgg
     9721 ccaccgaagc cgcgcatgac ccgctggcag taagccagac ccagtccggt cccatcggac
     9781 ttgccggccg atcggaacgg ttcgaacagc cgggccagca tgctcgccgg gatacccgga
     9841 ccggtgtcgc gcaccttcac ctgctgcctc tcgacggtga tcgtcacctc ggcttgcggg
     9901 cacagcgcca cgtaatacag cgcgttcttg atgaggttga acagcacgaa caggtaggct
     9961 gtttcatccc cccggaaact gaagtcctcg accacacgga cgctgaccct ggagcgttcc
    10021 gcgtcggtct catagccgta ttcctgcgcc gccttgtacg tcgcttcggc ggcggataca
    10081 tacgaaaagg cggccgtgtc catcggcttc tcgctgacct catcgagcgt catcgcgata
    10141 acctgcaagc cgcgcttgac ggccaactcg ctttcggcga ggtgcctgta cagggcgtcg
    10201 acctcgtgaa gcgccagtgg ccgggcctgc cgggaggtgg tgggcgccgg cagggcctgc
    10261 tgcatccttt cgaggttgtg cttgatctgg ctgagcgggt tgcgcatctc gtgcgcgatc
    10321 gagccggcaa gggacgcata cccttcgcgc attgcctcgg ccgccgcctg gcgtccggcg
    10381 aacttgaaaa aactgcctcc gatcaccacg agtaccagca ggggcaagcg cgccacatac
    10441 tccatgggga tgctggggtt cggatcaacg gccaggtaca gcaggacgcc gcccgagatg
    10501 cccacgacca gcatgacgat ggtgttgcgc cagtccgtca tgagaatcac gaaaaacgcg
    10561 accatcagcg tgttgccgac cgcggccgcg ccgccgccgt ttttcagtga cgtgaaaacc
    10621 agaaaaaccg gcaggctgaa gatcagcgcc ggatacgaga acgcgatgta gtacggcttc
    10681 aacctctgcg gccaccggtc cttcagcacc aggagcacgc aaacaagtgt tgccgccagc
    10741 ctgatccagg agtcatcgta gggcggcgag gacttggtga accgcagcac atagaaaatc
    10801 gggaatccga aagcacccac aagccccgcg taccgcagca tctgcgggcc atgctggtgg
    10861 tacagcttgt agtgggcaaa cagctgcttc acgaagttca atgcgatgcc cctgccggaa
    10921 cgatccgcgc cttgtcgggc ttgacgccgg cgagcgagtc caatacctga gccgcatagc
    10981 cctggatcaa ggtgtagctc ttttcggtac ccgcgagcac cacgtccttg gcgaaagccg
    11041 taaagccgaa gcgtcggtac agccggctca ggacgtgcga acacgtcgca tagagatcat
    11101 gcgcttcggt gtggcgggaa agatacgaca gcgacaggaa aagacaccgc ttcaacgcgt
    11161 caggatcgct cctgtattcg ggggcgagca ccagcctgcc cacctcccag cttcctttac
    11221 ccatcgtcac gcggggcgaa gccggctgcg ccagaagcgt ctcggtcagg gtcaggccgc
    11281 ttccgatcgg cacgaccctg atggtgccta tgatttcccc gcgcagttcg aaggcaccga
    11341 caaaaccgca ctcgtctctt ttttttcgag agctagaaaa ttggtcagtg catgcgaatg
    11401 caccgacagg tcgatggctt tcctgaggtg caggattcgc tcgatctcgt caggggtttt
    11461 caggtgccgg agcccgaact cctcgacgga aagtcgatgg ctttctgcgc acgccggttg
    11521 cgcggcggcg gctccggact cgaaaaacag gccgtgctga ctcgctgact gcgaagcatg
    11581 gccgctcatg ccgacggcga ccgaccggcc agccgaggac ccagttgaac tgatcattat
    11641 ttttcctctc ccgttcactt gaaggacggc aagaacccca ggctcgacgg gtgctggctg
    11701 ccaacctcag gaattatcgg cattttgcgg cgcaacgcaa cagcagaacc cacggggcct
    11761 cccgaatccg ccggtcgtcg ccgacgcgcc ggtgccaatg cccttgctcg cccgcccgga
    11821 tgtccaagcg gggacaatgt gccatgaacc aagtcgcaaa tgccgtggat gcccccgtgc
    11881 gccggctgga cctgggcggc ctcgcttggc gcaacagttt tgccggactg ggcccggcgt
    11941 tctacaccga gctggaaccg atcccgctgc cctccccgta cctggtcggg ctgaatcgtc
    12001 ccctcgcagc cgaattgggg ctttccgccc ggcttcttgc cgggcaggag ggcatcgagg
    12061 cattcacggg caacctgccg atcgccggca gccgtcccct ggccagcgtc tacagcggcc
    12121 accagttcgg cgtctgggcg ggccagctcg gcgacggccg ggccattctc ctgggcgaaa
    12181 tcgaaacacc ggccgggccg caggaattgc agctcaaagg cagcggactg acgccgtact
    12241 cccgcatggg cgatggccgc gccgtgctgc gatccagcat ccgggaattc ctcggttccg
    12301 aggcgatgcg gggcctgggc attccgacca cccgcgcgct gtgcgtcacc ggctcggacg
    12361 caccggtgcg gcgcgagggg atcgaaaccg ctgccgttgt gacccgggtg gcgcccagct
    12421 tcgtgcggtt cggccatttc gagcatttct cggcgcgcga gcagtacggc gagctggcaa
    12481 agctggccga ctacgtgatc gacagcttct atccggaatg ccgcgcctcg agccggttct
    12541 ccggcaatgc ctatgccgcc ttgctggaac aggtcagcga gcgcacggcg gccatggtcg
    12601 cccaatggca ggccgtgggt ttctgccatg gcgtcatgaa caccgacaac atgagcatcc
    12661 tgggcctgac catcgactat ggtcccttcc agttcctcga tgctttcgac cccggacaca
    12721 tttgcaacca cagcgacgag cagggacgct acgcctacaa caagcaaccg aacatcgcct
    12781 actggaacct gttctgcctg gggcaggcgt tgctgccgct gatcggcgac caggagcttg
    12841 ccctggccgc cctggagtcg tacaaggccg tgtttccccg tgagctggag gcgaggatgc
    12901 gggccaagct cggcttgagc gatgcgcgag cggcggatcg cgagctgatc gagggcatcc
    12961 tcaagctgct ggcagccgac cgcgtcgact acacgatctt ctggcggcga ctgtcgcgcc
    13021 acgcggcggg cgacgccgct gatacagttc gcgacttgtt cctgcagcgc gatgcattcg
    13081 acgcctggtt gatacagtat tcggaccggc tggacccggt tggccgcatc gaggcggccg
    13141 atctcatgct caagaccaat ccgaaatacg tgctgcgcaa ccacctgggc gagttggcga
    13201 ttcgccaagc gaaacttaag gactattccg aagtcgaaga cttgctggcc ctggtgcaag
    13261 ccccatatga cgagcatgcg ggccacgatg aaaaggccgg ttttccgccc gattgggctt
    13321 cacagatcga aatcagctgc tcctcatgac ctacaagata gagaagtccg acgccgaatg
    13381 gaaagcgctg ctggcacaga agggcgccga gccgggcgcg ttcgaagtga ctcgccacgc
    13441 cgccaccgag cgcccgttca ccggcaagta cgaagccgtg tgggacgacg gcagctatca
    13501 ctgcttgtgc tgcggcgcca agctgttcga ctcggtcgcg aagttcgatg ccggctgcgg
    13561 ctggccgagt ttttcggaag cgctgccggg cgccatcaag gaaatcaccg accgcagtca
    13621 tggcatgacg cgctccgaaa ccgtctgcgc gcagtgcggc gcccacctcg gccatgtttt
    13681 tcccgacggc ccgagcgata ccggcctgcg ctactgcatg aactcggcct ccctggactt
    13741 caagcccgaa caaggcaaat aaccgctctc atgaaactgc tgatcgactt tttccccatc
    13801 atcctgttct tcgtcgcctt caaggtcgcg ggcatctata tagcgaccgg cgtggcgatc
    13861 gtggcgacca tcgcccagat cgtctggctg cgccaccgcc acggcaaggt cgaaccgatg
    13921 cagtgggtca gcctgggtgt gatcgtgctg ttcggcggcg cgaccatcgc cctgcagaac
    13981 gagaccttca tcaaatggaa acccaccgtg ctgtactggc tgatggccgg gaccctcgcg
    14041 gccggccagc tgctgttcca caagaacctg ctcaagaccc tgatggggtc ccagatggag
    14101 ctgccggatg cggcctggcg gatcacgaac tggagctgga tcgtgttctt cgccctgatg
    14161 ggggtactga acctctgggt ggcctataac ttcgaaacga acacctgggt caacttcaag
    14221 ctgttcggcg gcctggggct gatggcgctg ttcgtgatcg gacaggcctt gtacctgggg
    14281 cgctacctca agaccgacca ggtgccggag ccatgagtgg aaccggtgtc acggccgagg
    14341 cgatgcgcca acggctgtcg gaactcctgg cgccctcgat gctcgaagtg atcgacgaaa
    14401 gcttccagca tgaggggcac cccggggcgg atgggaccgg ctttggcacg cacttccggg
    14461 tgcggatcgc ctcgcctttg ttcgccggcc gctcgcgcgt ggcgcgtcat cggcttgtgt
    14521 atgatgcgct gcaagttttt gtcgaccggg gcgttcacgc cctcgcgatt gaaacctctg
    14581 acccaccctc ctgactccca agggaacttc aatgaagcaa gtttttctgg ccgccacggc
    14641 ggcagccctt atcggcatgg ccgcgcccgc gctggcgcag aacgtggcca tcgtcaatgg
    14701 caagcccgtg cccaaggccc gagtcgacgc gctcgccgcc cagttggcca agtccggccg
    14761 gccgatctcg ccggaaatgc agggccagct caaggatgag gtcatcgcgc gcgagatctt
    14821 catgcaggag gcgcaaaaac gcggcctgga gggcagcgac gacttcaagg cccagatgga
    14881 actggctcgc cagaccctgc tgatccgcga gctcttcgcc gattaccaga agagcaaccc
    14941 ggtgaccgac gccgacatca aggccgagta cgacaagttc gcggcggcca acggcggcaa
    15001 ggaataccgt gcccgccaca tcctggtgga gaaggaagac gaggccaaga ccatcatcgc
    15061 gtcgctcaag aagggcggca agttcgacga gatcgccaag aagcagtcga aggatccggg
    15121 ctccggcgcc aacggcggcg acctggactg ggccaatgcg gccagctacg tgcccgagtt
    15181 ctcggctgcc ctggtcaagc tgaacaaggg ccagaccacc gaggccccgg tcaagaccca
    15241 gttcggctgg cacgtgatcc gcctggtgga cattcgcgag gcgcagttgc ccaagctgga
    15301 agcggtcaag ccccagatcg cccagcagct gcagcagcaa aagctcgcca agttccagga
    15361 agagctgcgc ggcaaggcga agatcgagtg agatttgtca tcccggactc gatccgggat
    15421 ccatggctcg agtgaagtgg attgcggggc cgggccggag cgtgatgcct cggtacgtgc
    15481 atagcgcagg cccggcattg ggctcgctgc actggacagc tgcgcgcccc gggcgttacg
    15541 cgagcccagc ccgcaatgac agccatcgag ctgcggccag tatcccgatc atcaccggct
    15601 ggtgcagcat gtaccacgtc aggctccagc gcccgagcca cgccaagggg gccgcagccg
    15661 ccggcaagcg cgcctgaagc aggtccttcc ggcgcgccag aaaccactgt ccggccgcca
    15721 cgccccacca catcacgccg agccacggga aaagcggcac gtagtcctcg gtgaccggct
    15781 tgcggctgat cagccccagc cagttccagg tcatatggtt gagaaaatca agagccggcc
    15841 attgaacatg ggcgaacaac gccagcggtt tgaccgcaag cgcgacggcg ccggccagcc
    15901 acagccagcg gccccagccg gccgtcaacc gcacgatgac cagcatcacg gcgatgccgt
    15961 gcagcacgcc gaagtagatg aagctcttcg ggaacatcca gtacgagccg gcggtgacca
    16021 gcagcgcaca gccggcgatc tgcaaccagc gcttgcagaa acggccccag ctctggccct
    16081 gcgcgaacgc aaaggcctgc cccaggcccg cgcaaaacag gaacaagctg acgatggccg
    16141 tgcgctgcca ggtccagaac gggtcggtgt agaaattctg cttcagccag ccgaaatagt
    16201 tgaggtcgaa gcagaaatgg aataccgtca tccagacgat cgcgattccc cgcaaggtgt
    16261 cgatggtggc gagtcgtgcg ggtggcatgg cgccaagtgt acgcaggcga tgcagcatgt
    16321 ttcgggcgcg accgtggcga acagaggacc cgccgacccc acgctcggcc gggcacgctg
    16381 tggccgaatt gccgggcgct caccgggttg acgaagcggc cgaggggcgc ttcaatccgg
    16441 tggcatacca ataacccaag gaaaagtcgg tgcttcgctt cgtttcccgt gcacggccgg
    16501 ggcaaaaggt ggagttctga gaagcgtcag gaagcgagta cgcgatgaat aaacctgccc
    16561 gttccgccgc tgagctggcc gcgcccgtgc tcgacgcatt gaaccggggc aatgtcattg
    16621 aagccttcaa gctgatgcgt gctgcgagcg ggctcggctt gcgggaggcc aagcaggctc
    16681 tggatgcgta ccggcgcggc aagcagccgt ccgcagcgca gccggagttt ccccgggcat
    16741 tgccgggcgg atcgctgccg cccagcgtcg tggaagccct gcagcgaggc aacaagatcg
    16801 aggccgtcac gcgcctgcgc cagcagaccg gcctgggact gaaggaggcg aaggatgcgg
    16861 tggaactgtt ccgccagttg cacccgcgac ccgacgatcc gtcacccggc gaggtgcgcg
    16921 attccggcgg tgcgctcgtg tgggtggccg ttgccctggc tgtcgtattc ctcctggcct
    16981 accggatctt ccagcgcctt ggctgacgca cggcaagctc gacttaccga cccctggggc
    17041 ctcgttcagt ccggcccgcc gttgaccccg atgaacgccg gcgggtttgg gcgtgactga
    17101 agcgccccga cccccgtccg acggcgactg gcacctgcgc gaggcgcacg aggtcgcgcg
    17161 ggcccatgag gtcaatccga taaccgggct gcaagagcac caggtgctcg agcgcgccga
    17221 gcgtcatggc cgcaacgagt tgccggtgac cgggggccgc cgcagcctct ggtcgctggc
    17281 actcgaccag ttcagcgatt tcatgatcct ggtgctgatc gccgcggcgg tggtctcggg
    17341 cctcatcggc gacctgatcg acacactggc gatcctcgtc atcatcgtgc tcaatgcggt
    17401 gatcggcgtg gttcaggcgt ggcgcgccga ccgggccctg gcggcgttga agcagctggc
    17461 cgcggcgcag gccaccgtgg tgcggggtgg gcaagtccgg caagtgcccg cgcacaacct
    17521 ggtccccggc gacgtcgtgc tgctggaggc cggcaaccag gtgccggcag acctgcgact
    17581 gatcgagatc gcgcaactga aagtcgacga gtccgcgctg actggcgagt cggtcaccgt
    17641 cgccaagcac gcgggtgttc tgggcggtgc actgcacgcg ctcggcgacc ggttgaacat
    17701 ggccttcaag ggcacgacgg caacccacgg gcgcggccgc ggcgtcgtgg tggcaaccgg
    17761 catgtcgacc gagctgggca aggtggccgg cctgctgggg caggaagccg accagagcac
    17821 gcccctgcag cgccggctcg ccgccttcgg caagcggctg gccatcgccg tgctggcgat
    17881 ttgcgccgtc atcctcggcg tgggcctgct gcgcggcgaa ccgctgctgc tgatggtcct
    17941 cacggcgctg agcctggccg tggccgccat tcccgaggcc ttgcccgccg tcgtcacggt
    18001 gctgctggca ctgggcgcgc ggcggatggt ggccgtgaac gcgctggtgc gacgcctgcc
    18061 gtcggtcgag acattggggt cggttaccta catctgcacc gacaagaccg gcaccctcac
    18121 gcagaaccgc atgcaggtgg aaagcctggt cgtggacggc gaagccgcac cggttacggg
    18181 caccgaatcc gagccggccc gcagagtgtt gctgcgcgca ctggccttgt gcaacgacgc
    18241 caccccgtca gcggacggcg gctggcaggg cgaccccacc gaggtcgcac tggccgaagc
    18301 cgcggccgcg gcggggctgg acaagaccgc gctgcagtcc gaatggccgc gtgtctcgga
    18361 actgcctttt gacgccgagc gcaagcgcat gaccaccgtg cacggcacgc tacaaggata
    18421 cgtcgcatac accaagggcg cgcccgaatc ggtcatcccg ctgtgcggcg acgcctgccc
    18481 cggcggcctg gaccgcgcgg cctggctgca gcgggccgaa gcc
//
LOCUS       DASWIY010000164         8507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2324780, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000164 DASWIY010000000
VERSION     DASWIY010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8507
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2324780"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..952)
                     /locus_tag="VGA59_06380"
     CDS             complement(32..952)
                     /locus_tag="VGA59_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9719342.1"
                     /translation="MDKLKQLESFVSVATRGSLTAAAKAEGVAPAIMGRRLDALEERL
                     GVKLLLRTTRRISLTHEGSAFLEDCQRLLADVANAEASVSAGGVKASGHLRITAPAGF
                     GRRHVAPLVPKFRELHSDVTISLNLSDRVVDLAGEGYDCAVRVGDLPDSSLVSMRMAD
                     NRRLCVATPTYLKRHGVPQHPNELVKFDCLTLSTDASQTRGWAFRVPKADGAEVVHLK
                     PGGPLDCSDGQVLHDWCLAGYGIAWRSTWEVESEVAAGRLAEVLAEFAAPPNGIYAVF
                     PQRKHLPLRVRLWIDFLKHNYSQPDFWKAS"
     gene            complement(1005..2192)
                     /locus_tag="VGA59_06385"
     CDS             complement(1005..2192)
                     /locus_tag="VGA59_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9719343.1"
                     /translation="MNTARLPYDGVRVVEFTHMVMGPTCGMVLADLGAEVIKVEPIAG
                     DNTRRLLGSGAGFFPLFNRNKKSIALDLQRPEAREAALRLIATADVVSENFKPGTMKK
                     LGLDYASLAGLNERLIYVSLKGFLPGPYDHRTALDEVVQMMGGLAYMTGRPGDPLRAG
                     TSVNDIMGGIFGAIGAMAALALREKTGKGQEVQSALFENNVFLVAQHMMQFAVTGQPA
                     APMPGRISAWGVYDVFTVKDGEQIFLAAVSDTQWALFCDAFGFGDLKVDPRLKTNNDR
                     VLARDWMMPILRSRLADRSAAELSAIFEKNALPFAPITRPEQLFDDPHLQQTGGLAPM
                     TLPDGRTTAVPLLPLTIGGERPGVRLDPPHLGEHSAELLRELGYSAEEISAMERAKLT
                     LAS"
     gene            2416..3447
                     /locus_tag="VGA59_06390"
     CDS             2416..3447
                     /locus_tag="VGA59_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9719344.1"
                     /translation="MRIQSTVSTASKTVLGLIAIVALAGPARADVLDDVKSHGTLKCG
                     VTLAAPGFSAEDNSGKREGFDVDLCKAIAASTLGDATKIQLVPMDLKTAFAGLPAGAV
                     DVLTHRFTWTQSRDVGGLKFAKVMFFDGQGFAVTKKSKANKIADLNNATICTAQGSTS
                     ELIVADYFRAHKLKYKIVTFASQEQAWDAFVGKRCDAAINDRSGLAARLAKLPNRDDF
                     KVLGETVSNEPIGPIVAQGQPRWETVVRFTLNALVAAEELGVTQANVDEQRSSQNPEV
                     QRLLGVSGDFGPKMGLAADWAYKAIKAVGNYGEIWDRNVGPKTPLRLERGRNALVANG
                     GLMMAIPIR"
     gene            3593..4597
                     /locus_tag="VGA59_06395"
     CDS             3593..4597
                     /locus_tag="VGA59_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9719345.1"
                     /translation="MTGLPRTKMSRAVLMERLPGYVGQLVLLVLVIWLVWRGFSNFLG
                     NVRALGVSTGYGFLHTEAGFEIAQSLIAFSPASTYLDAVIVATLNTLLVIALAGFFST
                     LIGLCIAFGRLAPHPLVRGASSAYVEVFRNIPLLLQIFFWYFSAMSLLPGVADSLQLG
                     AVLLNNRGLFLPHLQLMPFGFDVPRVEGFNVAGGFALLPELIALTLGLSIYNSTFLAE
                     IFRAGILSVPRGQTEAAATVGLSGVRAALLITLPQALRLALPPAAGQYQTLAKASSLA
                     AAIGYPDIMQIVGGTVLSQTSQALEAMALVVLLYATINLFIALVVNLANRRLLKREGR
                     "
     gene            4701..5693
                     /locus_tag="VGA59_06400"
     CDS             4701..5693
                     /locus_tag="VGA59_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX9719346.1"
                     /translation="MGVLSMLLLAAFIAAGWRFFQWAIVYAHWSGSSSDACPGDGGAC
                     WAFIVARWKPWLVGDYPSDQLWRAWACFIAFALFWTWVVRRSHEASMQRVLLAFIALP
                     VAFFLLLAGGGPLPAVAPTRWGGLLLTMMATLATFATALPSGLALAIGRRSRLPVVSW
                     LCAAFVESMRSVPLLAVLFIAATLLPMFLPNGWNVGLFTRAVVAFALFNAAMAAEVFR
                     GGLQSVGRGQIEAATTVGLSDFAALRLIVLPQAVTAVVPALVNIMIAVIKETTLLSVI
                     GVNDLLGAVENGAKSPGWVGEANILTSGHVFLALAYLSVCYGLSRYSRRLEARH"
     gene            5707..6486
                     /locus_tag="VGA59_06405"
     CDS             5707..6486
                     /locus_tag="VGA59_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX9719347.1"
                     /translation="MTTRPIATSDPATPVVRMDGINKWYGSFHALRDIDLQVAPGERI
                     VICGPSGSGKSTLVRTVNLLEPFQQGRLQVCGVEVGADDITRAARLAVNRRVGMVFQQ
                     FNLFPHMTALQNLTVGPIWGSGVSRREAHERAIHYLERVGLMDQAHKHPAQLSGGQQQ
                     RVAIARTLCTKPQVVLFDEPTASLDPEMVSEVLQTMESLASEGMTMMIVTHEMGFARR
                     VADRVIFMDQGQIIETAPPGDFFERPQSPRLKSFLGQVLTH"
     gene            6544..7524
                     /locus_tag="VGA59_06410"
     CDS             6544..7524
                     /locus_tag="VGA59_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9719348.1"
                     /translation="MTVIDVHTHMFTARWLELLKQEGGIYNIQTRPDGQQEIFRGITP
                     VVLPQKGHFDYGLRIAHMDQAGIDISIVSLTCPNVYWGGVDVSCRAARESNDNMAEAQ
                     ARYPDRIRWFTSLPWEYPQRAIEELERTCANGAVGVMVLANIAGRSLTDPLFAPIWAE
                     IDRRALPVLVHPTDPPGVDMMDMSKFDLSWSVGFMFDTTLAITRMIFEGFFDQYPNMK
                     VIASHGGGTLPYLVGRFEKGDEVELASRRQMKRKPTDYLRHIYYDSITYDVRSLQYLI
                     SIVGEDHVMLGTDWPHWVHDTKGAFANTAQLPEGQMKKVRAGNAGRVFGF"
     gene            7706..>8507
                     /locus_tag="VGA59_06415"
     CDS             7706..>8507
                     /locus_tag="VGA59_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719349.1"
                     /translation="MSAVFPPFDPERFEEKRRRDAKRWNLPFAAVKLQCLDVTGAEVP
                     GASASGFVCSEADGLYLYTCWHVVSGYGSPFRLSVKGELSPRAFLKLEAQAVEDRAPG
                     MQVIGGKQDLVLPIMDLASNARRCLWLQEGHHRPHEDLNNVGVRVPTFFDAVKIKLGA
                     EVEMPPVSIHTPDKRSLWLHIGQTILIAGFPHGYSAVGGHQPTPVVLTRHVAAVTVQG
                     NIHCFLADGPAAPGMSGGPVYAVKDDGDIVLVGIYAGLIHPGAGKVQFP"
BASE COUNT         1396 a         2722 c         2798 g         1591 t
ORIGIN      
        1 atgtgagcgt aggcaagcag ggattcgagg atcatgaggc cttccagaaa tcaggctggc
       61 tgtagttgtg cttgaggaag tctatccaca gtcgcacccg cagcggcagg tgcttgcgtt
      121 gcgggaagac cgcgtagatg ccgttgggcg gcgcggcaaa ttcggccagc acttcggcga
      181 gccggcccgc cgccacttcg ctttccacct cccaggtgct gcgccaggcg atgccgtagc
      241 cggccaggca ccagtcgtgc agcacctggc cgtccgagca gtccagcggg ccgcccggct
      301 tgaggtgaac gacttcggcc ccgtccgcct tcggcacgcg aaaagcccag ccccgggtct
      361 gcgacgcgtc ggtggacaac gtcaggcagt cgaacttcac cagttcgttg ggatgttgcg
      421 gcactccgtg gcgtttcagg taagtggggg ttgccacgca caggcgccgg ttgtccgcca
      481 tgcgcatgct gaccagcgac gagtccggca ggtcgcccac gcgcacggcg cagtcgtagc
      541 cctcgcccgc gaggtcgacc acccggtcgc tcaagttcag cgagatggtg acgtccgaat
      601 gcagctcgcg aaacttcggt accaggggcg caacgtggcg tcgcccgaag ccggccggcg
      661 ccgtgatgcg caggtggccg ctggccttga cgccgccggc gctgacactg gcttcggcat
      721 tggcgacatc cgccagcagg cgctggcagt cctcgaggaa agcgctgccc tcatgggtca
      781 gcgagatccg tcgcgttgtg cgcagcagca gtttcacccc caggcgctct tccagcgcgt
      841 ccagccgccg gcccatgatg gcgggcgcca cgccctccgc cttggcagcg gcggtcaggc
      901 tgccccgcgt ggcgaccgac acgaaggatt caagctgctt gagtttgtcc atccagcgca
      961 gattatgcgc attccggtta tgaatgaacg aaaaaccgca gcgattaact cgcgagagtg
     1021 agcttcgcgc gttccatggc gctgatttcc tcggcgctat agccgagttc gcgcaggagt
     1081 tcggcgctgt gttcccccag gtgcggcggg tccagcctca cgccgggccg ttcgccgccg
     1141 atcgtcaggg gcagcagggg caccgccgtg gttctgccgt ccggcaaggt catgggggcc
     1201 agcccaccgg tttgctggag atgcgggtcg tcgaacagct gttcgggccg cgtgatcggc
     1261 gcgaacggca gcgcgttttt ctcgaaaatc gcgctgagtt ccgccgcgct gcggtcggcc
     1321 aggcgcgagc gcaggatcgg catcatccag tcgcgcgcca ggacccggtc gttgttggtt
     1381 ttcaggcgcg ggtccacctt gaggtcgccg aagccgaaag cgtcgcaaaa cagcgcccac
     1441 tgcgtgtcgc tcacggccgc caggaaaatc tgctcgccat ccttcacggt gaagacgtca
     1501 tacacgcccc aggccgaaat acggcccggc atcggcgcgg ccggctggcc cgtcacggcg
     1561 aactgcatca tgtgctgcgc caccaggaac acgttgttct cgaacagcgc gctctgcact
     1621 tcctggccct tgccggtttt ctcgcgcagg gccagcgcgg ccatcgcgcc gatcgccccg
     1681 aagatgccgc ccatgatgtc gttgacgctg gtgccggcac gcagcggatc gccgggacgg
     1741 ccggtcatgt aggccaggcc gcccatcatc tgcaccactt cgtccagcgc ggtgcgatgg
     1801 tcgtacgggc cgggcaggaa ccccttgagg ctgacgtaga tgaggcgctc gttcagcccg
     1861 gcgaggcttg cataatccag gcccagcttc ttcatcgtgc cgggcttgaa gttttcgctc
     1921 accacatcgg ctgtcgcgat cagccgcagc gcggcctccc tggcttccgg cctttgcagg
     1981 tccagcgcga tgctcttctt gttgcggttg aacaggggaa agaatcccgc gccggagccc
     2041 agcaggcggc gcgtgttgtc gccggcgatc ggctcgacct ttatcacctc ggcgccgaga
     2101 tcggccagca ccatgccgca ggtcgggccc atgaccatgt gggtgaactc gaccaccctc
     2161 acgccgtcgt aaggcaaacg ggctgtattc attgccggac attttgcccg gaatggctgg
     2221 cgcggtggcg agcgagcagc ctccgcggcg gggccggtga gcgggggtct ggaccgacgc
     2281 cagctcaggc tcgacggcag cacgctgtcg gaagcggcag cagatacact gttacgcggc
     2341 gggcagccat gcactcgccg aaccgaaccg aggaccgcgt ggaagcggtc ttattccaaa
     2401 cacgcggaga cctccatgcg catccaaagc acagtttcaa cagcaagcaa gacggttctg
     2461 ggattgattg ccatcgtggc gctggccggt cccgcgcgcg ccgacgttct ggacgacgtc
     2521 aagtcccatg gcacgctcaa gtgcggcgtg acgctcgctg cgccaggctt ttcggccgag
     2581 gacaattcag gcaagcgcga gggcttcgat gtcgacctgt gcaaagccat tgccgcctcc
     2641 acgctgggtg atgcaaccaa gatccagctc gtcccgatgg atctgaaaac agcgttcgcc
     2701 ggcctgcccg caggcgccgt cgatgtgctt acgcaccggt tcacgtggac tcagagccgg
     2761 gacgtcggcg gcctgaaatt cgccaaggtc atgttcttcg acggccaggg cttcgcggtc
     2821 accaagaagt ccaaagccaa caagatcgcc gacctgaaca acgcgaccat ctgcaccgcg
     2881 cagggcagca cgtcggagct gatcgtcgcc gactacttcc gcgcccacaa actcaagtac
     2941 aagatcgtga ctttcgcatc gcaagagcag gcctgggacg ccttcgtcgg caaacgctgc
     3001 gacgccgcga tcaacgaccg ctcgggcctg gccgcgcggc tggcgaagct gccgaaccgg
     3061 gatgacttca aggtgctggg agaaactgtg tccaacgaac cgatcgggcc gatcgtggcg
     3121 caggggcagc cgcgctggga aaccgtggtc cggtttacgc tgaacgcgct ggtggccgcc
     3181 gaggagctgg gcgtgacgca ggccaatgtc gacgagcaac gcagttcgca aaaccccgaa
     3241 gtgcagcggc tgctgggcgt gagcggcgac ttcggtccca agatgggcct ggcggccgac
     3301 tgggcctaca aggcgatcaa ggcggtgggc aactacggcg agatctggga ccgcaacgtc
     3361 ggccccaaga cgccgctgcg cctggagcgc gggcgcaacg cactggtcgc caatggcggc
     3421 ctgatgatgg cgatcccgat ccgctgagcg aacgtggtca aagcagcgcc actcgttgtt
     3481 acgctcctag cccaggcggc cagcctgggc gtcgtcacgc gcctagattg gcgctgtttt
     3541 gaccacgttc gcactcgcaa ggtagtgtga acaggcctta acgccccttc gcatgacggg
     3601 gcttccccgc accaagatgt cccgcgccgt tctgatggaa cggttgccgg gctatgtcgg
     3661 ccagctcgtg ctgctggtgc tcgtgatctg gctggtctgg cgcggctttt ccaatttcct
     3721 ggggaatgtg cgcgccctgg gcgtgtccac cggctatggt tttctgcaca ccgaagcggg
     3781 cttcgagatc gcgcagtcgc tgatcgcgtt cagcccggca tcgacctacc tggacgccgt
     3841 gatcgtggcc acgctcaaca cactgttggt gatcgcactg gccggtttct tcagcaccct
     3901 gatcgggctt tgcatcgcat tcggacgact ggctccgcat ccgctcgtcc gcggcgcgtc
     3961 gtcggcttat gtcgaggtgt ttcgcaacat ccccttgctg ctgcaaatct tcttctggta
     4021 tttctcggcg atgtcgctgt tgccgggggt ggccgacagc ctccagctcg gcgccgtgct
     4081 cctgaacaat cgcgggctct tcctgccgca tttgcagctg atgccgttcg ggttcgacgt
     4141 cccgcgcgtg gaaggcttca acgtcgccgg cggcttcgct ctcctgcccg agctgatcgc
     4201 gctgacgctc ggcctgtcga tctacaacag caccttcctg gccgagattt ttcgcgcggg
     4261 gatcctgtcg gtgccgcgcg gccagaccga agccgcagcc accgtgggtc tgagcggcgt
     4321 gcgcgcggcc ctgttgatca ccttgccgca ggcgctgcgg ctggcgctgc cgccggccgc
     4381 cgggcagtac cagacgctgg ccaaggcgtc ttcgctggcc gcggcgatcg gttatccgga
     4441 catcatgcag atcgtgggcg gcaccgtgct gagccagacc agccaggcgc tggaagcgat
     4501 ggcgctggtg gtgttgctgt atgcgaccat caatctgttc attgcccttg tcgtgaatct
     4561 cgcgaaccgc cgcttgctca agcgagaagg gcggtgactt gcatttcgtg cgccaacaac
     4621 tgattgagcc gcatcccgtc ccggtgcttg cgaccccggc gctcgtgcgt tggcgcgcgc
     4681 tctggtttcc aacccccgcg atgggcgtgc tatcgatgtt gctgctggcg gcgttcatcg
     4741 cggccggctg gcggttcttt cagtgggcca tcgtgtacgc gcactggagc ggcagcagca
     4801 gcgacgcgtg cccgggcgat ggcggcgcgt gctgggcttt catcgtcgcg cgctggaagc
     4861 cgtggctggt gggcgactat ccatcggacc agctgtggcg ggcctgggcc tgctttatcg
     4921 cgtttgccct gttctggacc tgggtggtgc gccgctcgca tgaggcgagc atgcagcggg
     4981 tgctgctggc cttcatcgcg ctgcccgtgg cgttctttct gctgctggcg ggcggcggtc
     5041 cgctgccggc ggtggcgccg acgcgctggg gcggcctgct gctgaccatg atggcgacac
     5101 tcgcgacctt cgccacagcc ttgccaagcg gcctggcgct ggcaatcggg cgccgctcgc
     5161 ggctgccggt ggtgagttgg ctgtgcgcgg ccttcgtcga gagcatgcgt tcggtgccgc
     5221 tgctggcagt gcttttcatc gcagccacgc tgctgccgat gtttctgccc aatggctgga
     5281 acgtcggcct gttcacgcgt gcggtggtgg cattcgcgtt gttcaatgcg gcgatggcgg
     5341 ccgaggtgtt tcgcgggggc ctgcaatcgg tcggccgcgg gcagatcgaa gcggccacca
     5401 ccgtgggcct gagcgacttc gcggcgctgc gcctgatcgt gctgccgcag gccgtgacgg
     5461 ccgtggtgcc ggcgctcgtg aatatcatga tcgccgtgat caaggaaacc accctgctgt
     5521 cggtgatcgg cgtcaacgac ctgctgggcg cggtggaaaa cggtgccaaa tcgcccggct
     5581 gggtcggaga ggccaacatc ctgacctcgg gccacgtgtt cctggcgctg gcttatctgt
     5641 cggtgtgcta cgggttgtca cgctatagcc ggcggctcga agcccggcac tgaatccgtt
     5701 agaagcatga ccaccagacc tatcgcgact tccgacccgg ccacccccgt cgtgcgcatg
     5761 gatggcatca acaagtggta tggctccttc catgcattgc gcgacatcga tctgcaggtg
     5821 gcgcccggag agcggatcgt gatctgcggg ccctcgggct cgggcaagtc gacgctggtg
     5881 cgcaccgtga atctgctcga accattccag cagggccgcc tgcaggtctg cggcgtggag
     5941 gtgggcgccg atgacatcac gcgggccgcg cggctggcgg tgaaccgccg ggtcggcatg
     6001 gtgttccagc agttcaacct gtttccccac atgacggcgc tgcagaacct caccgtgggt
     6061 ccgatctggg gcagtggcgt gagccggcgc gaggcccacg agcgcgccat ccactatctg
     6121 gagcgtgtcg ggctgatgga ccaggcgcac aagcacccgg cccagctgtc cggcggccag
     6181 cagcaacggg tggcgatcgc gcgcaccttg tgcaccaagc cgcaggtggt gctgttcgac
     6241 gaacccaccg cctccctcga cccggagatg gtcagtgaag tgctgcagac gatggagagc
     6301 ctcgcgagcg agggcatgac catgatgatc gtcacgcacg agatgggctt cgcgcggcgc
     6361 gtggcggacc gcgtcatctt catggaccag gggcagatca tcgagactgc gccgcccggc
     6421 gactttttcg agcggccgca aagcccgcgt ctgaagtctt tcctcgggca ggtgctgacg
     6481 cattgagtgc ctgccacgca gtttttccag ttcgtatcgc atcgatatca ggagaccgca
     6541 ttcatgaccg tcatcgacgt ccacacccac atgttcaccg cacgctggct ggaactgctg
     6601 aagcaggaag gcggcatcta caacatccag acccggccgg acggccagca ggagatcttt
     6661 cgcggcatca cgccggtcgt gctgccgcag aaaggccact tcgactatgg gctgcgcata
     6721 gcccacatgg atcaggctgg catcgacatc tccatcgtgt cgctcacctg ccccaacgtg
     6781 tattggggcg gcgtagacgt cagctgccgg gcggcgcgcg agtcgaacga caacatggcc
     6841 gaggcccagg cgcggtaccc ggaccgcatc cgctggttca cttcgctgcc ctgggagtat
     6901 ccgcagcgcg ccatcgagga actggagcgc acttgcgcca acggggctgt cggcgtgatg
     6961 gtgctggcca acatcgccgg ccgcagcctc accgatccgc tgttcgcgcc gatctgggcg
     7021 gagatcgaca ggcgcgccct gccggtgctg gtgcacccca ccgacccccc gggcgttgac
     7081 atgatggaca tgtcgaagtt cgacctgagc tggtccgtgg gcttcatgtt cgacaccacg
     7141 ctggcgatca cgcgcatgat cttcgagggc ttcttcgacc agtacccgaa catgaaggtg
     7201 atcgcctcgc acggcggcgg caccctgccc tacctggtgg gccgcttcga gaaaggcgac
     7261 gaagtggaac tggcctcacg ccgccagatg aagcgcaagc cgacggacta cctgcgccat
     7321 atctattacg acagcatcac ctacgacgtg cggtccctgc agtacctgat ctcgatcgtc
     7381 ggtgaggacc acgtgatgct gggcaccgac tggccgcact gggtgcacga cacgaaaggg
     7441 gcgtttgcga atacggcgca gttgccggag gggcagatga agaaggtcag ggcggggaat
     7501 gcggggaggg tgtttgggtt ttaggtggtc gtggcccgcc gaagcggccg acaaggctgc
     7561 ttctggccgg aagcggccat cattgaactc ggacggcgtc tctggagctt ggcgcccccc
     7621 tttgatctgc tatacccttt gcagttgcga agctgacgtc cgtgttctct cgcagcatgc
     7681 ctgctcactg gttggggaga tgaccttgtc tgctgtgttc cctccgtttg atccagaacg
     7741 ctttgaggag aagcggcgcc gagacgcgaa aaggtggaac ttgccgtttg ccgccgtaaa
     7801 gctgcaatgc ctcgatgtga ccggcgccga agttcctggc gcttccgcga gcggctttgt
     7861 ctgcagtgag gcggacggac tctacctcta tacgtgttgg cacgttgtct ccggctacgg
     7921 ttctccattt cgtttgtcag tcaagggtga gctttcccca cgcgcttttc tgaagctgga
     7981 ggcacaagct gtcgaagacc gagcaccggg gatgcaggta atcggcggaa agcaggacct
     8041 cgtgctgcca atcatggatc tggcttccaa tgcacgtcgt tgcttgtggc tgcaagaagg
     8101 gcaccatcgt cctcacgaag atctgaataa cgtcggcgtt cgcgtcccaa cgtttttcga
     8161 cgcagtgaag atcaagttgg gcgcagaagt cgaaatgccg cccgtgtcaa tacacacgcc
     8221 cgacaagcgc tcactttggc ttcacatcgg gcagacaatc ttgatcgcgg gctttccaca
     8281 tgggtacagc gcagttggcg gacatcagcc tacgcctgtc gtcttgaccc gacacgtagc
     8341 cgcggtgaca gttcagggaa acatccactg ctttctcgca gatgggcccg cagcacccgg
     8401 tatgtctggc ggtccggtgt atgccgtcaa agatgacggt gacatcgttc tcgtcggcat
     8461 ctatgctggc ctcatacacc ccggcgctgg caaggtgcag ttccctg
//
LOCUS       DASWIY010000165         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_465918, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000165 DASWIY010000000
VERSION     DASWIY010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465918"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VGA59_06420"
     CDS             <1..598
                     /locus_tag="VGA59_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719350.1"
                     /translation="IQQVPNSAMTSLVAQVRIPRPGGKVANIVVLHKLFTVSGLKKST
                     EFTNRVIARSVQVQWGEGIVFRVMHPLDLLESRVHNAAGLLEDKGPHVLTQARWAIKV
                     AKAAVLKMAKDAALSQHAQVPGKRVGAAIKSIYGLAHSRAGRRLRTQHGIEVLDAIDI
                     KALATLAPAHARQLDAVEQAKLRRVEEDAPTSPAVRAS"
     gene            complement(791..1012)
                     /locus_tag="VGA59_06425"
     CDS             complement(791..1012)
                     /locus_tag="VGA59_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719351.1"
                     /translation="MDEYLKGIGAFALIIVGGAWLLRFFDERHAGNELKSCMAGIRAR
                     LASHPEDADMLRQEEHELLKNRPPRAKLF"
     gene            1278..2114
                     /locus_tag="VGA59_06430"
     CDS             1278..2114
                     /locus_tag="VGA59_06430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9719352.1"
                     /translation="MSAADLEGAQALVAESGWNQVRADWQLFIELGAAFKVMGRDGEV
                     AATAATLPYTGFGWISMVLVGKAHRRQGMATALLAHCMARLREDGLVPVLDATPAGRP
                     VYQPLGFHDGWPITRWRRVERGAAIDDTGGNAATSVRPLREGDWRDLAALDARAFGAD
                     RTALLRRLQSRSKGFACVAERTGRVAGYLLGRDGRQATQVGPVVARDPGLASALLAHA
                     LARIDGPVLLDVLDRHTAVPPQLTSAGFAVERSYTRMTLGCESTFGDADLMTAIAGPE
                     LG"
     gene            2360..2566
                     /locus_tag="VGA59_06435"
     CDS             2360..2566
                     /locus_tag="VGA59_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719353.1"
                     /translation="MIDSALTQFELRFQSLFDSGRGYSFPCDPEGHVDLNQLSDRARD
                     NYLYARAVVGRELAFPAVRPGGLH"
     gene            complement(2659..3294)
                     /locus_tag="VGA59_06440"
     CDS             complement(2659..3294)
                     /locus_tag="VGA59_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9719354.1"
                     /translation="MINIGIVDDHAIVRTGLKQFFSEQVDFRVVGEAANGREAIDLVR
                     TTPELDVLVMDLSMPGQSGMDVLGMIRAKAPDLGILILSGYPEAQFAMNLLRQGASGY
                     LNKDCEPTEIVKAIRAITLGGRYISSSVAELLGQQFSRKIEGPPHEQLSAREFQVFLK
                     LAKGETASEVATALSLSVKTVSTYRARLMEKMNLGSNSDLTYYAMKNSLID"
     gene            3393..3626
                     /locus_tag="VGA59_06445"
     CDS             3393..3626
                     /locus_tag="VGA59_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719355.1"
                     /translation="MKTPEMSDLLQEWRAADRAATAAEKAVLADSLRSPDAMGASPAI
                     YHLEKARRRRAMANDLFEVAMEQMAARAGALKR"
BASE COUNT          677 a         1127 c         1186 g          701 t
ORIGIN      
        1 catacagcag gtaccgaaca gcgccatgac ctcactcgtc gcgcaggtgc gcattcccag
       61 gcccggaggc aaagtggcca acatcgttgt gctgcataag ctgttcaccg tgagcgggct
      121 caagaagtcc acagagttca ccaaccgggt gatcgcgcgc agtgttcaag tgcaatgggg
      181 cgaaggaatc gtttttcggg ttatgcatcc attggatctc ttggaatcgc gcgtccacaa
      241 cgccgctggc ttgctcgagg acaaaggccc gcacgtgctc acccaggctc ggtgggccat
      301 caaggtggcc aaggcagccg tgctgaagat ggccaaagac gcagcactgt cgcaacacgc
      361 gcaagtgccc ggcaagcgcg tgggcgcagc catcaaatcg atctacggcc tggcgcacag
      421 ccgggctggc cgaaggcttc ggacgcagca cggaatcgaa gttctcgacg cgatcgacat
      481 taaggcactc gcgacactgg cgccggcgca tgcccgacaa ctggatgcgg tggagcaagc
      541 caagcttcgg cgcgtggagg aggacgcgcc aacctcgcca gcggttcgcg cctcgtaaag
      601 cgggcgtgct tcggcagcgg cgacgccgcg gcggatggcc aagccgcggc gcacgcaccg
      661 ctactcgaat ccgcattggc aaacggtgcg cttcggctgc atgccctcac cccagccctc
      721 tcccagaggg agagggggta ataccagagg gaccctcagc aacctgtcgc cgaaaaaagt
      781 gattgggcgg ttagaacagc ttggcccgcg gcggccggtt cttgagcagc tcgtgctcct
      841 cttgccgcag catgtccgcg tcttccggat ggcttgcaag ccgagcgcgt atgccagcca
      901 tgcaactctt gagttcattg ccagcatgtc gctcgtcgaa gaaccgcagc agccacgctc
      961 ctccaacgat gatgagcgcg aaggccccga tgcctttcag atattcatcc acgacagcct
     1021 tttggtgagg taacggtccc gctcatccag ggcggatgca cgcggttgct cactataggc
     1081 gctaatccat gtgatggcag cgcacttaca gttgcgcgtc gcgtcaaccg atctttccgg
     1141 tgcgagtcgg ggactctact aatacgtgat caagggtccg ctgcctaaac tgaagtgatg
     1201 ttgtttattt acataaccgg gcgggtgcca cgcgattaga cgctgtgcgg caatgccagg
     1261 gactggccat ttgcccgttg agcgccgcgg atctggaggg tgcgcaggct ctggtggcgg
     1321 aatcgggctg gaaccaggtg cgcgccgact ggcagctgtt catcgaactc ggtgccgcct
     1381 tcaaggtgat gggccgcgac ggtgaagtgg cggcgaccgc tgcgacgctc ccctacacgg
     1441 gcttcgggtg gatcagcatg gtgctggtgg gtaaggcgca ccgccgacag gggatggcca
     1501 ccgcgctgct cgcgcactgc atggcccggc tgcgcgagga cggcctggtg cctgtgctgg
     1561 acgccacacc cgcggggcgc ccggtctacc agccgcttgg tttccacgac ggctggccga
     1621 tcacgcgctg gcgcagggtc gagcgcggcg cggccataga cgacacgggt ggaaatgcag
     1681 caacgagcgt gcgccccttg cgcgagggcg actggcgcga tcttgctgcc ctggacgcaa
     1741 gggcgtttgg ggcggatcgc acagcgctct tgcggcggct ccaatcgcga tcgaaaggat
     1801 tcgcctgcgt ggccgagcgg acaggccgag tcgcgggcta tctgctgggg cgggatgggc
     1861 gccaggcgac gcaagtcgga ccggttgtgg ctcgcgatcc gggcttggca tccgcgttgc
     1921 tggctcacgc actggcccga atcgatggcc ccgtgttgct ggacgttctc gatcggcaca
     1981 ccgcggttcc cccgcagctc acgagcgccg gattcgcagt cgaacgaagt tacacacgca
     2041 tgacgttggg ctgcgaatcc acctttggcg atgcggacct gatgaccgcc atcgccggcc
     2101 cggaacttgg ttgaaatcac aaccataacg cccacggcta cggcgtcacg tgttccggga
     2161 gcaggttgcc ctgcgcgctt cgaaggcacg ggctctgcct gcccgagtcc ctttcgctgt
     2221 aggtcatcgc cgcgttcgca tcgctaagtt cggcaacgca cagacgttga tcgatgctaa
     2281 agcgcaacct gttttcatgg ggatttcccc cgacgtgtca aactcaacga gacacgcagc
     2341 gactacagga gacgttgaaa tgatcgattc cgcgctgacg caattcgagc tgcgtttcca
     2401 gtccctgttc gacagcggcc ggggctattc atttccatgc gaccctgaag gccatgtcga
     2461 tctgaaccag ctgagcgacc gggcccgcga caattatctc tatgcccgcg cagtggtcgg
     2521 ccgcgaattg gcgtttcccg cggttcggcc tggcgggctg cactgagttg gcgtagcgcg
     2581 atcggtcgtt gctcggggcc gcgctgcgtt accagagcgc agagtcacgc atgctttcga
     2641 atcttctgag agcggcgctc agtcgatcaa cgagttcttc atcgcgtaat aggtcaggtc
     2701 gctgttcgag ccgaggttca tcttttccat cagccgggcg cggtaggtgc ttaccgtctt
     2761 gacgctgagg gaaagggctg tggcaacctc gctcgcggtt tcgcccttgg cgagcttcag
     2821 gaaaacctgg aactcgcgcg cggataactg ctcgtgaggg gggccctcga tcttgcggct
     2881 aaattgctgt cccagcagtt cggcgaccga gctggagatg tagcgtccgc cgagcgtgat
     2941 ggcgcggatc gctttgacga tctccgtcgg ctcgcaatcc ttgttgaggt atccgctggc
     3001 gccctggcgc agcaggttca tcgcgaactg tgcttcaggg tagcccgaca ggatcaggat
     3061 gccgaggtcc ggcgccttgg ctcggatcat gcccaggacg tccatcccgc tttgccccgg
     3121 catggaaaga tccatcacga gcacgtccag ctcgggggtg gtgcgaacga gatcgatcgc
     3181 ttcgcggccg tttgcggctt ctcccacgac ccggaaatcg acctgctcgg agaagaactg
     3241 cttgagtcca gtccggacga tggcgtggtc gtccacaatg ccaatattga tcatggcttc
     3301 attctgctct cttgcggatg agtgctttga gctcaaacac cacctcgatt agccgtctgg
     3361 ctgataaaat agctcgtccc catgggtgac ttatgaaaac acccgagatg tctgatcttt
     3421 tgcaggagtg gcgcgcggca gatcgagctg ccaccgcagc ggaaaaggct gtgcttgcgg
     3481 attctctgcg ctcccccgat gcaatgggag cttcgccggc catctaccac ctcgaaaaag
     3541 caaggcgccg ccgcgccatg gcgaacgacc ttttcgaggt cgcaatggaa caaatggctg
     3601 cgcgggcagg ggcgctgaag cgatagcgac gggcgctgaa gcgatagcga cggtggcctt
     3661 gcctgtcatc ccgggcttga cccgggatcc a
//
LOCUS       DASWIY010000166         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_465951, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000166 DASWIY010000000
VERSION     DASWIY010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465951"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..541)
                     /locus_tag="VGA59_06450"
     CDS             complement(155..541)
                     /locus_tag="VGA59_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719356.1"
                     /translation="MADDEPNIVISLEYLLRREGHEVIAAYDGRQALDAVARERPDLI
                     LLDVMMPLKTGFEVCQHLRATTAFHDIPIIMLTARGRDTDVARGLALGADAYLTKPFS
                     TRELIAKVAELLEARSRGCSASAGPQ"
     gene            complement(826..1179)
                     /locus_tag="VGA59_06455"
     CDS             complement(826..1179)
                     /locus_tag="VGA59_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719357.1"
                     /translation="MMFDVSTAVTWLLFLALFPLAFFWLRRAWRILVRRDFSEVALKR
                     GESPPNAERFAPYEMIINLIAGTIAVVVIVAVALGQVDYDTWTAIAGSTIWCKFFASF
                     ALSRHAHAAAARAST"
     gene            complement(1337..>3077)
                     /locus_tag="VGA59_06460"
     CDS             complement(1337..>3077)
                     /locus_tag="VGA59_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX9719358.1"
                     /translation="WARAGSTSEFYAAGGSVHPVMNGMATAADWMSAASFISMAGLIS
                     NMGYGGGLFLMGWTGGYVLLAMLLAPYLRKFGKFTVPQFIGDRFYSKSASTVAVICLL
                     TASITYIIGQMTGVGVAFSRFLGVSAETGIYVGMAIVFAYAVFGGMKGITYTQVAQYV
                     VLILAYTIPAIFISLQLTGNPIPQLGLGSDLVGQDVSLLAKLDQVVTDLGFGKYTTTT
                     AGSTLNMFMYTLSLMIGTAGLPHVIMRFFTVAKVKDARSSAGWALVFIAILYTTAPAV
                     AAMAKMNLIRTVTPGVVMAGADAYGKEATIEYDKRPDWMKRWEKTGLLKFQDKNGDGR
                     IQYYNDKTKNVEVAKKAEAAGWKGNELTVNADIIVLANPEIALLPNWVIALVAAGGLA
                     AALSTAAGLLMAISSAVSHDLIKGVFKPGISEKGELMAGKIAMAVAIVISGWLGLNPP
                     GFAAGTVALAFGIAASSLFPAIMMGIFSRKMNKEGAMAGMLAGLLITLFYVFAHKGLF
                     FIKGTEYVGLIGGPNPFFGITPEAFGAVGAMVNFGVAFLVDKVTREVPSHIREMVEAV
                     RIPRGSKAVDGAH"
BASE COUNT          563 a          997 c         1021 g          496 t
ORIGIN      
        1 tggatcccgg gtcaagcccg ggatgacagc ccatgcccgg gatgacagcc catgcccggg
       61 atgacaaata cctctgtcat tgcgggcttg acccgcaatc caggaattca tggatcccgg
      121 gtcaagcccg ggatgacaag cctgaggaga tttgtcattg cggcccggcg ctcgcggaac
      181 agcctcgcga gcgcgcctcc agcagctcgg ccactttggc aatcagctcc cgcgtggaga
      241 acggcttggt caggtaggca tcggcgccca acgcaagccc cctggccacg tcggtgtcgc
      301 ggccgcgcgc ggtgagcatg atgatcggga tgtcgtggaa ggcggttgtc gcccgcaggt
      361 gctggcacac ttcgaagccc gtcttcagcg gcatcatcac gtccagcagg atcaggtcgg
      421 gccgctcgcg ggcaaccgca tccagggcct gccggccgtc gtacgcggcg atgacctcat
      481 ggccctcgcg ccgcagcagg tactccagcg agatcacgat gttcggttca tcgtccgcca
      541 cgagaatcct tgcgctcagg atggctctcc tttcatctca gttgctccac cgcgcgccgc
      601 gcgacccgcg acgcatcaaa acgcgtggcc aatgcggggg ccaccgcgga accggctttg
      661 ccgggccgct ggtgggcctt gcaggccgca tggccgcgag acgcggccat tcctcgacct
      721 gtccaaaccg acgcaggtcg gtttggagcc gcaggtctcg gcccccttga gggggagagg
      781 gcgaagcccg aggggcatcg ccgcaggcgc ttcgggggtg ggctttcaag tgctggcccg
      841 cgcggcggct gcgtgcgcat gccgcgaaag cgcgaaactg gcgaagaact tgcaccagat
      901 ggtgctgccc gcgatggccg tccaggtatc gtagtcgacc tggcccagcg ccacggccac
      961 gatcaccacc acggcgatcg tgccggcgat caggttgatg atcatctcat agggggcgaa
     1021 gcgctcggcg ttggggggcg actcgccgcg cttgagcgcc acctcggaaa aatcgcgcct
     1081 gaccaggatg cgccatgcgc ggcgcaacca gaaaaacgcc aacggaaaaa gcgcgagaaa
     1141 cagcagccat gtcacggcag tgctcacatc gaacatcatg tgcaactcct ggggtcccgg
     1201 cctggctcag ccgggcgaaa ggcgaaaaaa acatgggccc tgccaatgac gccggcaggg
     1261 ccccgattgt cgctccgtcc ggcttcaggg cacagccctt ggccggacga aattcaagcc
     1321 tgctgtaaca gcatcatcag tgcgcgccgt ccacggcctt ggaaccgcgc ggaatgcgca
     1381 cggcctcgac catctcccgg atgtgcgacg gcacttccct ggtgaccttg tccaccagga
     1441 aggccacccc gaagttcacc atcgcgccca cggcaccgaa ggcttcgggc gtgatgccga
     1501 agaacgggtt ggggccgccg atcaggccga cgtactccgt gcccttgatg aagaacaggc
     1561 ccttgtgcgc gaacacgtag aacagggtga tgagcaggcc cgccagcatg ccggccatgg
     1621 cgccttcctt gttcatcttg cgcgagaaga tgcccatcat gatcgcaggg aacagcgagc
     1681 tggccgcgat gccgaacgca agcgccacgg tacccgccgc gaatcccggc ggattgaggc
     1741 ccagccatcc ggagatgacg atggccacgg ccatcgcgat cttgccggcc atgagttcgc
     1801 ccttctcgct gatgccgggc ttgaacacgc ccttgatcag gtcatgcgac acggcggacg
     1861 agatggccat cagcaggccg gcggcggtgg agagcgcggc ggccaggcca ccggcggcga
     1921 ccagcgcgat cacccagttg ggcagcagcg cgatctccgg attcgccagc acgatgatgt
     1981 cggcgttcac ggtgagctcg ttgcccttcc agccggcggc ttccgccttc ttggcgactt
     2041 cgacgttctt ggtcttgtcg ttgtagtact ggatgcggcc gtcgccgttc ttgtcctgga
     2101 acttcagcag gccggtcttc tcccagcgct tcatccagtc gggacgcttg tcgtactcga
     2161 tcgtggcttc cttgccgtag gcgtcggcac cggccatcac cacgcccggt gtgacggtgc
     2221 ggatcaggtt catcttggcc atggcggcca cggcgggagc cgtggtgtac aggatggcga
     2281 tgaacaccag cgcccagccg gcggacgaac gcgcgtcctt gaccttggcc acggtgaaga
     2341 atcgcatgat cacgtggggc aggccggccg tgccgatcat cagcgacagc gtatacatga
     2401 acatgttcag cgtgctgccg gcggtggtcg tggtgtactt gccgaagccc aggtcggtga
     2461 cgacctggtc gagcttggcc agcaacgaca cgtcctgtcc gaccaggtcg gaacccaggc
     2521 ccaactgggg gatcgggtta ccggtgagct gcagcgagat gaagatcgcc ggaatggtgt
     2581 aggccaggat cagcacgaca tactgcgcca cctgcgtgta ggtgatgccc ttcatgccgc
     2641 cgaacaccgc gtaggcgaac acgatggcca tgccgacgta gatgccggtt tcggccgaga
     2701 cgcccaggaa gcgcgagaac gccacgccca cgcccgtcat ctggccgatg atgtacgtga
     2761 tggaggcggt caagaggcag atcacggcca cggtgctggc ggacttcgag tagaagcggt
     2821 cgccgatgaa ctggggcacg gtgaacttgc cgaacttgcg caggtagggc gcgagcagca
     2881 tggccagcag cacgtagccg ccggtccagc ccatgaggaa caggccgccg ccgtacccca
     2941 tgttggagat caggccggcc atggagatga aggatgccgc cgacatccag tcggccgcgg
     3001 tcgccatgcc gttcatcacg gggtggacgc tgccgccggc ggcgtagaac tcgctggtcg
     3061 agccggcacg ggcccag
//
LOCUS       DASWIY010000167         4639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_93863, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000167 DASWIY010000000
VERSION     DASWIY010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93863"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VGA59_06465"
     CDS             <1..553
                     /locus_tag="VGA59_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX9719359.1"
                     /translation="AWPSTTPPRTGQPASQATYAGRQPTSPGVPGEATPTAGARSRST
                     TWSAKVFEEIEWRRFEAVCEKLFGQAGFEARTRSHGADGGVDIWLHSRHAAGPAAIAQ
                     CKHWHGRQVGVKELREFYGVMASHKLKRGTYATTSTFTADAIRFARENGISVLDGNGL
                     LALIARRTPEQQAELLQTAYQGE"
     gene            1096..3327
                     /gene="glgB"
                     /locus_tag="VGA59_06470"
     CDS             1096..3327
                     /gene="glgB"
                     /locus_tag="VGA59_06470"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX9719360.1"
                     /translation="MRDRTGSAASAADSATIAEAEVQLAADLRDGLCADPFGNLGAHS
                     GIQSGHFRLRVFVPGSDGVTVLARDTRQAVARLQAVDDSGLFTGDVALAHRGQYLLQI
                     DSPLGQRAIEDPYRFGPWLGDTDVWLLAEGRHLRPWDKLGAHPCTIDGVAGVAFAVWA
                     PNARQVALAGDFNLWNSRCHPMRLRSECGVWEIFVPALPSGTLYKFDVLGADGQRQLK
                     SDPYARSSELPPGHASRVVALPEPVTGRAAVRQQGNALSAPVSIYEVHAGSWRRPGGA
                     LPDWDYLAQHLVPYVTGMGFTHIELLPINEHPFYASWGYQPTSLFAPTARYGTPEAFR
                     AFVAAVHDAGLQLILDWVPGHFPSDAHALARFDGTALYEHEDPREALHRDWNTLIYNF
                     GRNEVRNFLVGNALFWIERYGVDGLRVDAVASMLYRDYSRAEGEWIPNRFGGRENLEA
                     IAFLRDVNKVIGEQAPGTATMAEESTAFPGVTKPPWADGLGFHYKWNMGWMHDTLGYF
                     ALDPIVRKYHHDQISFVMMYAYSEHFILPLSHDEVVHGKGSLYGRMWGDPWHKRANLK
                     ALFALMYAQPGRKLMFMGSELGQQHEWNHDAELDWGLQQDAGHAGVQRLVRDLNRLYR
                     SRPALHARDDETAGFAWIEVNDRDHSVFGFLRHGHAPGQQMLVACNFTPVPRHGYRMG
                     VPQGGVWLECLNTDSDHYGGSGVGNLGAVQSEAVPAHGMPMSLSLTLPPLGVVWFEPG
                     SAA"
     gene            3333..>4639
                     /locus_tag="VGA59_06475"
     CDS             3333..>4639
                     /locus_tag="VGA59_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9719361.1"
                     /translation="MQPQICLRGQPWPLGATRMTWQGHEGVNLAVFSRHATCVHWCLF
                     DAATREETGRLPLPECTDGIWHGFLPGLPVGQLYGLRASGPYQPTAGHRFNAARLLID
                     PYARELSGGVAGLANEVGFIGDAAGQEQPDAHDNATRVPKARVVDLERELAAGAAIAP
                     GPRIAPERTVLYEAHVKGLTRLHPGVADDLRGTYAGLASAPMLAHYEKLGVTTLCLLP
                     VQHHIDEKHLLDLGLANYWGYNTLAYFVPEPGYAAATTAEGVREEFRAMVDQLHRHGL
                     EVVLDVVYNHTAEGDARGPTLCWRGLDNASSYALDGAGQYLNFTGCGNALNLGEPRMV
                     QFVMDSLRWWVQAFGVDGFRFDLATALGRDPMLHHRFNPGAALITAMAQDVVLARVKR
                     IAEPWDLGPRGYQLGAFPAGWQEWNDRFRDTARSYWLGHDCTPG"
BASE COUNT          747 a         1606 c         1527 g          759 t
ORIGIN      
        1 tgcgtggccc tccaccaccc cgccgcgcac cggccaacca gcgtcgcaag cgacgtatgc
       61 cggtcgacag cccacctcgc ctggcgtccc aggcgaggcc actccaaccg ccggcgctcg
      121 atcgaggagc acgacctgga gcgcgaaggt gttcgaggag atcgagtggc ggcgtttcga
      181 agccgtgtgc gagaagcttt tcggccaggc gggcttcgaa gcgcgcaccc ggtcccatgg
      241 cgcggacggt ggcgtcgaca tctggcttca ttcgcgacac gccgcgggcc cggcggcgat
      301 cgcccaatgc aaacactggc acggcaggca agtcggcgtc aaggagctgc gcgagttcta
      361 cggcgtcatg gcctcgcaca agctcaaacg cgggacttat gccacgacat ccacgttcac
      421 ggccgacgca atacgcttcg caagggaaaa cggcatcagt gtcctggacg ggaatggatt
      481 gctggcactg atcgcccggc gcacccccga acaacaggct gagttgctgc agaccgcgta
      541 ccagggcgag tagcgcattc cgcgcgatcc tccgccatca accgggcccc gcgtcggcgt
      601 gtttcgaatt gctccgcgct ttcgcccgcc cttcgacgca tggcttgggc catgacccgg
      661 ttttgttcgc ttcgccgacg gtcgactccc ttcgcatggg gtcttgccgg caccggcgcc
      721 ggatcggtgt tgacgatacg tctggagcag ggttgctcgg tgtacagcac cgatcccctg
      781 gaccagcact tgtagaccgt ttgcgcgctt gccggcgccc agacgccaat gcacaacaat
      841 gcacttagca accaaacatt tcgattcacc ggcaccatgc gtccttcgct aactgctgag
      901 cttgaatcgt agcggcctat ggtcacccgg cggctccgga ttgtttccat ccgctacttt
      961 gtgtacgccc gcccgccttc gttgatcggg taccggtgat gcgccgcggc aagctggtct
     1021 atgtgtgatt ctgcgaccgc ccccgctgcc gcgcgagtcg ggccacaatg gtcgtccaga
     1081 aaaatggatg cgccgatgag agacagaacc gggtcggcag ccagcgctgc cgacagcgcc
     1141 acgattgctg aagccgaggt ccagctggcc gcagacctgc gcgatggact gtgcgccgat
     1201 cctttcggca acctcggtgc gcactctggc attcaaagcg ggcactttcg attgcgcgtg
     1261 ttcgtccccg gcagcgacgg cgtcacggtg ctggcgcggg acacccggca ggccgtggcc
     1321 cggctgcaag ccgtcgacga cagcggcctg ttcacgggcg acgtggcact ggcgcatcgc
     1381 ggccagtacc tgctgcagat cgactcgccg ctcggccagc gcgctatcga ggacccgtac
     1441 cggttcgggc cctggctggg cgacaccgat gtctggctgc tcgccgaagg gcgccacctg
     1501 cgcccctggg acaagctggg cgcgcacccc tgcaccatcg acggcgtggc cggcgtggcg
     1561 ttcgcggtct gggcgcccaa tgcccgccag gtcgctctgg cgggggactt caacctgtgg
     1621 aacagccgct gccatcccat gcgcttgcgc agcgagtgcg gcgtctggga gatcttcgtt
     1681 ccggcgcttc cgtccggcac gctgtacaag ttcgatgtgc tgggtgccga cggccagcgg
     1741 cagctcaaga gcgatcccta cgcgcgcagt tccgagttgc cgccgggcca cgcgagccgg
     1801 gtcgtggcct tgcccgaacc ggtcaccggg cgcgccgccg tgcggcagca gggcaatgcc
     1861 ctgagcgccc ctgtcagcat ctacgaagtc catgccggtt cgtggcgacg gcccggcggc
     1921 gccctgcccg actgggacta tctcgcgcag catctggtgc cctacgtgac cgggatgggc
     1981 ttcacccaca tcgagctgct gccgatcaac gagcatccgt tctacgcctc ctggggctac
     2041 caacccacca gcctgttcgc gcccacggca cgctacggca cacccgaggc gttccgggcc
     2101 ttcgtcgcgg ccgtgcatga cgccgggctg caactgatcc tggactgggt gccgggccac
     2161 tttccgagcg atgcgcatgc cctggcgcgc ttcgacggga ccgcgctgta cgaacatgag
     2221 gatccgcgtg aagcactcca ccgcgactgg aacaccctga tctacaactt cggccgcaac
     2281 gaggtgcgca atttcctggt gggcaacgcc ttgttctgga tcgaacgcta tggcgtcgat
     2341 gggttgcgcg tggacgcggt ggcctccatg ctgtatcgcg actacagccg ggccgaaggt
     2401 gaatggattc cgaaccggtt cggcggacgc gaaaaccttg aagccatcgc cttcctgcgc
     2461 gacgtcaaca aggtcatcgg cgagcaggct cccggcaccg cgacgatggc cgaggaatcc
     2521 acggcgttcc cgggcgtgac caagccgccc tgggccgatg ggctgggctt ccactacaag
     2581 tggaacatgg gatggatgca cgacaccctg ggctacttcg cgctggatcc catcgtgcgc
     2641 aagtaccacc atgaccagat cagtttcgtc atgatgtacg cctacagcga acatttcatc
     2701 ctgccgctgt cgcatgacga agtggtccac ggcaagggtt ccctgtacgg ccggatgtgg
     2761 ggcgacccct ggcacaagcg cgccaacctc aaggccctgt tcgccctgat gtacgcacag
     2821 cccggccgca agctgatgtt catgggctcc gagctgggcc agcagcatga atggaaccat
     2881 gacgccgagc tcgattgggg cttgcagcag gacgcgggcc acgccggcgt gcagcgcctg
     2941 gtacgcgacc tcaaccggct ctaccgcagt cgcccggcct tgcacgcgcg cgacgacgaa
     3001 accgcaggtt tcgcctggat cgaggtgaac gaccgggatc attccgtctt cggcttcctg
     3061 cgccatgggc acgcacccgg ccagcagatg ctggtggcgt gcaacttcac gccggtgccg
     3121 cgtcacggct atcgcatggg cgtgccccag ggcggagtgt ggctggagtg cctcaacacc
     3181 gattcggacc actacggcgg cagcggcgtc ggcaacctgg gagccgtcca gtccgaggcg
     3241 gtgccggcgc acggcatgcc catgtcgctg agcctgacct tgccgcccct tggcgtggtc
     3301 tggttcgagc ccgggtcggc cgcatgacac gcatgcagcc gcaaatctgc ctgcggggcc
     3361 agccctggcc gctgggtgcc acccgcatga cgtggcaggg ccacgaaggc gtgaacctgg
     3421 ccgtcttctc ccgccatgcc acctgcgtgc actggtgcct gttcgatgcg gcgacgcgcg
     3481 aagagaccgg ccggctgcca ctgccggaat gcaccgacgg catctggcat gggttcctgc
     3541 ccggcctgcc cgtggggcaa ctctacggac tgcgggccag cggcccttac cagcccacgg
     3601 ccggccaccg cttcaatgcg gccaggctcc tgatcgaccc gtatgcccgc gaactgtccg
     3661 ggggcgtggc cggcctcgcc aacgaagtgg ggttcatcgg ggatgcggcg gggcaggagc
     3721 aacccgatgc gcacgacaat gccacgcgcg tacccaaggc acgggtcgtc gacctggagc
     3781 gggagctggc cgcgggcgcg gccatcgcgc ccgggccgcg catcgcgccg gagcgcacgg
     3841 tcctgtacga ggcgcatgtg aaggggctga cccgcctgca cccgggcgtt gcggatgact
     3901 tgcgcggcac ctacgccggc ctggccagcg cgcccatgct ggcgcactac gagaagctgg
     3961 gcgtcaccac cctttgcctg ctgccggtgc agcatcacat cgacgaaaag cacctgctcg
     4021 acctgggcct ggccaactac tggggctaca acacgctggc ctacttcgtg cccgaaccgg
     4081 gctatgccgc cgcgaccact gccgagggcg tgcgcgaaga attccgcgcc atggtcgacc
     4141 agctgcaccg gcatggcctg gaggtggtgc tcgacgtggt ctacaaccac acggcggaag
     4201 gcgacgcccg gggccctacc ctgtgctggc gcgggctgga caacgccagc agctatgcgc
     4261 tggacggcgc cggccagtac ctcaacttca ccggctgcgg caacgcgctc aacctgggcg
     4321 agccgcggat ggtgcagttc gtgatggaca gcctgcgctg gtgggtccag gccttcgggg
     4381 tcgacggttt ccgtttcgac ctggccacgg cgctgggccg cgacccgatg ctgcaccatc
     4441 gtttcaaccc gggtgccgcg ctgatcacgg caatggcaca ggacgtcgtc ctcgcacgcg
     4501 tcaagcgcat cgccgaaccc tgggacttgg ggccgcgcgg ttaccagctc ggcgccttcc
     4561 ccgccgggtg gcaggagtgg aacgaccgct tccgcgacac cgcgcgttcc tactggctgg
     4621 ggcacgactg cacgccggg
//
LOCUS       DASWIY010000168        12436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_836143, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000168 DASWIY010000000
VERSION     DASWIY010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 12436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12436
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836143"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VGA59_06480"
     CDS             complement(<1..192)
                     /locus_tag="VGA59_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430110.1"
                     /note="with UgpACE is involved in the uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9719362.1"
                     /translation="MKLQLSVAALAVAFALPAQAQTQIQWWHAMGGPLGEWVNDLAKD
                     FNASQKDYVVVTTYKGSYGE"
     gene            367..4413
                     /locus_tag="VGA59_06485"
     CDS             367..4413
                     /locus_tag="VGA59_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEX9719363.1"
                     /translation="MNAPTTLNRLMAAADGDAPRLREIPYNYTSFSDREIVIRLLGPV
                     AWTVLNRLREERRTGRSARMLYEVLGDIWVVQRNPYLQDDLLDNPKRRRLLVDALHHR
                     LAEVEKRRTPDTDPERDALVGDLLKAAGRAVDAFDASFRDMAELRRRTQRALRRFTAR
                     DNIKFDGMSRVSHVTDATDWRVEYPFVVLTPDTEAEMAGLVKGCIDLGLTIIPRGGGT
                     GYTGGAVPLTWKSAVINTEKLEAMTEVEFVAIPGHEQEVPTVWTEAGVVTQRVSDAAE
                     RAGYVFAVDPTSAEASCVGGNIAMNAGGKKAVLWGTALDNLVSWRMVTPQAQWLEITR
                     LDHNLGKIHDAEMAGFELRYFEADGKTPVRTERLDIPGRTFRKEGLGKDVTDKFLSGL
                     PGIQKEGCDGLITSARWVVHRMPQHTRTVCLEFFGNARDAVPSIVEIKDFMFAEQSRS
                     GVLLAGLEHLDDRYLRAVGYSTKSKRGTDAKGGPSSALPKMVLVGDIAGDNADDVARA
                     TSEVVRIANSRSGEGFVAISPEARKKFWLDRKRTAAISKHTNAFKINEDVVIPLPRMA
                     EYTDGIERINIELSLANKIALADQLEAFFAGGNLPLGRHDDAGEIPSAELLEDRVAQA
                     LELVRGVRVLWRGWLQDVEKLFPQLQDHTLRASWKTQIRAPLQNIFAGHAFGPILEEA
                     NAIHKRVLKGRVWVALHMHAGDGNVHTNIPVNSDDYEMLQTAHRAVKRIMVLARSLNG
                     VISGEHGIGITKLEFLTDEELQPFADYKQQVDPEGRFNKGKLLRRPSPPPSPASGRGG
                     QSLPPASGGQSFPPAGAGQSLPLPLAGEGRGEGIMYADLTNAYTPSFGLMGHESLIMQ
                     QSDIGAIADSVKDCLRCGKCKPTCSTHVPRANLLYSPRNKILATSLLVEAFLYEEQTR
                     RGVSVKHWQEFEDVADHCTVCHKCEPPCPVNIDFGDVSMNMRNLLRKMGQKSFRPGNA
                     AAMLMLNATSPETIKLLRGAVVGIGFKAQRLANDLLRGLARKQATRPPSTHEAAPVKE
                     QIIHFVNKKLPGGLPKKTARALLDIEDKDYVPIIRNPKATTAETEAVFYFPGCGSERL
                     FSQVGLATQAMLWHAGVQTVLPPGYLCCGYPQRGSGQFDKAEKIITDNRVLFHRVANT
                     LNYLDIKTVVVSCGTCYDQLQGYEFEKIFPGSRIIDIHEYLLEKNITLPAGQGGFMYH
                     EPCHNPMKLQDSLKTVKALVGDKVRKSERCCGESGTFGVSRPDIATQVRFRKEEELRK
                     DEAALREAMKGAAGGAGAVAEKGNLKILTSCPSCLQGLTRYGNDLQNGLLEADYIVVE
                     MARQILGDNWMPEYVHAANSGGIERVLV"
     gene            4410..4862
                     /locus_tag="VGA59_06490"
     CDS             4410..4862
                     /locus_tag="VGA59_06490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX9719364.1"
                     /translation="MKVAGCPLCDGPGGVAVFEGPKFRVVRAEEAGFPAFYRLVWSDH
                     ASEFTDLSAADRRLCMEAVAQVEQALRAHLQPAKINLAALGNAVPHLHWHIVARYEWD
                     SHFPAAVWAAAQREVPPGRLAAVEQLRPDLEKDLAGRLANKTGNRTAS"
     gene            5014..6360
                     /locus_tag="VGA59_06495"
     CDS             5014..6360
                     /locus_tag="VGA59_06495"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX9719365.1"
                     /translation="MARGALGICLLLAAAAASAQPAGEVEFSRGVGFAQSPGQAPRTL
                     GKGLPLAEGDRLTTAEGASAIIKLQDGTRMTVRPNSELLLQQYRFKENAPDNNMLMQL
                     LRGGFRAVTGLISKNAPNAARIQTSTATIGIRGTDFDARLCTRDCGAESARVTASARP
                     NAVLASARVVSSRGEVNAVDGSGQRRRLVDGGSVYPGDLVETGSATQAVLAFRDDTRI
                     TLGATTRFRVDNFVYDDRNAGEGRFLVSLLKGSVRALTGLIAKANKRNVGFSTATATI
                     GIRGTGLDINCTGACAGEPGDSDSGLTLFAWLGSIVVTPTGQSALEALQAGQGLFISP
                     SGIRTINNQPDMDGPRPDTVTVPPKLFSNDNVSDDEEGLFVFVRDGHIEIAVAGETLH
                     LGRGEAGFAGHAGDARRPVNIPKFIDFDQLPLPTARNPLLVTVLSESGIRPNNQCR"
     gene            6429..8057
                     /locus_tag="VGA59_06500"
     CDS             6429..8057
                     /locus_tag="VGA59_06500"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEX9719366.1"
                     /translation="MVSGYGAPRPRNGRARLGLLAWAAMGLSAPALAQAPANPPMAAL
                     APSPVFTIRGFKVTGENPLGDGETARVLAPFLRSGATLQTLQKATAALETALRERGYG
                     LHRVALPPQEVGDTVTLTIVKFSVAKVHIEGRSIYDEANVRRALPELREGDSPNFKRL
                     AIQTAIANENPNKQVQVGLRESEEPDKIDATITVKESRPWNFALGLSNAGSRSTGRDR
                     LTVSGAHTNLFNLDHQFIGAYTTSLERPQDVRQLGLSYKVPLYGLGGVAGAAYTRSDV
                     VGDFGTFTSTGAGHTFGASYTLYLPPAGGRRSYVLLGLEDKVFDATVINDTVVGADRR
                     SRPVTLGYSARTGSDAASWGYNLDFAANTGSGRNNDLASYRSEDPRITTVHWKALRGG
                     VSYSAAFAKTWTWAARGQFQYSPDVLISGEQFGLGGLGSVRGTDIERPVTGDKGLSAT
                     AEVGTPELKPGLRLLGFLDAGWLGNNSPNGTHKPGTDRLASVGLGLRYAREPFAVSLD
                     YGRLVVGSRVPLAFNAAAPQKGDDRVYLNLSVRF"
     gene            8079..8489
                     /locus_tag="VGA59_06505"
     CDS             8079..8489
                     /locus_tag="VGA59_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX9719367.1"
                     /translation="MAGLNSDSPTPRSLTLHGQSRVLEVSFADGAQFRLPFELMRVYS
                     PSADVAGHGPGQEVLQTGKRDVEITGLEPVGNYAVQPTFSDGHDTGIYSWDYLYFLGQ
                     QQEQLWADYERRLKQAGVDRDAPMPDKAAPSCGH"
     gene            8577..9308
                     /gene="ubiE"
                     /locus_tag="VGA59_06510"
     CDS             8577..9308
                     /gene="ubiE"
                     /locus_tag="VGA59_06510"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX9719368.1"
                     /translation="MSSTHFGFESVDEREKARRVRGVFDSVAPRYDLMNDLMSMGLHR
                     AWKAYTVLAAHVRPGDHVLDIAGGTGDLSLAFARQVGATGRVVHTDINEAMLRTGRDR
                     LLDAGVSLPTLVCDAEKLPFTPDSFDLVSVAFGLRNMTHKDAALAEMNRVLKPGGKLL
                     VLEFSKIAKPLEKAYDWYSFKVLPRLGKLVAGDDASYRYLAESIRVHPGQEELKALMK
                     KCGFGHVDYHNLTGGVVALHVGIKC"
     gene            9334..10314
                     /locus_tag="VGA59_06515"
     CDS             9334..10314
                     /locus_tag="VGA59_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEX9719369.1"
                     /translation="MKFLSMVFVVLLAFASFDSEARRMGGGGSFGRQSGNVTQRQAGQ
                     PPASPAQNQQAAPANQPGAGANAAAAAPRRPFGGMLGGLAAGLGLAWLAHSLGLGEAF
                     AQFMLIALAVMVAMVVIGLVMRSRGAGQTGGRYNLQSAGAPADLAQAPRQYSPDKVGN
                     DASARPWERGSMAFEAAKYGQGSGVQIGSGLAGPQSWGVPPGFDTEGFITAAKRNFES
                     LQDAWDRSDISSLRAMMTDEMLGEIKSQLADREKDQAGKPNKTEVVMLDAQLLGIEDT
                     GSDYMASVEFSGMIREEPSAGPSPFREVWNMTKSKSGGSGWLVAGVQALQ"
     gene            10403..10975
                     /locus_tag="VGA59_06520"
     CDS             10403..10975
                     /locus_tag="VGA59_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719370.1"
                     /translation="MATQSPFPFLDTLFERLAGGLQPPAWMVEEVQRRLVLLFNHVLM
                     QEPEAQARLARQANRVVEARWRIFVMRLVATPAGLLDLAPGAAQPDLTLTLTEESPWE
                     LAQAALRGDNPPVRIAGDVQFANEINWLVEHVRWDLEEDLSRLVGDAPAHAMGTAARR
                     IVQALRQFTRPSPGSGQPPAPGPGPGQAGA"
     gene            10972..>12436
                     /gene="ubiB"
                     /locus_tag="VGA59_06525"
     CDS             10972..>12436
                     /gene="ubiB"
                     /locus_tag="VGA59_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEX9719371.1"
                     /translation="MTRYLRGIYIVWVALSYGLDELVLNSFQRPWLSRIARVLSFGRN
                     LKAPRGQRLRQALEHLGPIFVKFGQVLSTRRDLLPADVADELARLQDRVPPFDSAVAM
                     ATVERAFGRPVDTIFATFEREPVASASIAQVHFATLKDRNGQTRDVAVKVLRPGMLTV
                     IEKDLSLMRMMAGWLDRLSKEARRLKPREVVAEFSKYLHDELDLVREAAAAAQLRRNM
                     AGLNLVLIPEMLWDYCHPEVIVMERMVGVPISQVERLRQAGVNIPQLARDGVTIFFTQ
                     VFRDGFFHADMHPGNIQVSIEPATFGRYVSLDFGIIGTLTEYDKEYLAQNFTAFFRRD
                     YKRVAQLHIESGWVPPDTRVDELESAVRTVCEPYFDRPLKEISLGLLLMRLFQTSRRF
                     HVEIQPQLVLLQKTLLNIEGLGRQLDPDLDLWSTAKPFLEKWMLEQVGPQKLLDELRD
                     QAPRYAKLLPDLPLLLHQYLTQKPVDGRREMLELLAEQ"
BASE COUNT         2185 a         4106 c         4183 g         1962 t
ORIGIN      
        1 ctcgccatag cttcccttgt aggtggtgac gacaacgtag tccttttggc tcgcgttgaa
       61 atccttggcg agatcgttca cccactcacc cagaggtccg cccatggcgt gccaccactg
      121 gatctgggtt tgtgcttgcg ccggcagcgc gaaagcgacc gcgagggccg ccacggaaag
      181 ttgcagtttc atgggttctc ctggatagac cgctgaatgt taggaaccat ttgtgacagc
      241 ggatttaacc ggccgtccgg ttggtccaca agcggggttt ccataggtga aaggggtaat
      301 gctgcactgc ggtaacatct tccctcagcc gcgtgccatt cgttgaggct gaaatttcac
      361 ctaacgatga acgcacccac aacgctcaac cgactgatgg ccgcggccga cggcgacgcg
      421 ccgcgcctgc gcgagatccc ctacaactac acctccttct ccgaccggga aatcgtcatc
      481 cggctcctgg ggccggtcgc atggacggta ctgaaccgct tgcgcgagga gcggcgcacc
      541 gggcgctcgg cccgcatgct gtacgaggtt ctcggcgaca tctgggtggt gcaacgcaac
      601 ccgtacctgc aggacgacct gctggacaac cccaagcgcc gccgcctgct ggtggacgcg
      661 ctgcaccacc ggctcgcgga ggtggaaaag cgccgcaccc cggacacgga tccggaacgc
      721 gatgccctgg tgggtgatct gctcaaagcg gccggccggg cggtggatgc gttcgacgcg
      781 agctttcgcg acatggcgga gctgcgccgc cgtacacagc gcgcgctgcg gcggttcacc
      841 gccagggaca acatcaaatt cgacggcatg tcgcgtgtct cgcatgtgac cgacgccacc
      901 gactggcgcg tcgaataccc gttcgtggtg ctcacccccg acaccgaggc cgagatggcc
      961 ggcctggtca agggctgcat cgaccttggc ctgacgatca ttccgcgcgg cggcggcacc
     1021 ggctataccg gcggcgccgt gcccctgacc tggaagagcg cggtgatcaa caccgagaag
     1081 ctcgaggcga tgacggaagt ggagttcgtc gcgatcccgg ggcacgagca ggaggtgccg
     1141 accgtgtgga ccgaggccgg cgtcgtcacc cagcgggtgt ccgatgcggc cgagcgcgcg
     1201 ggctacgtgt tcgcggtcga tcccacgtcc gccgaggcat cttgcgtcgg cggcaacatc
     1261 gccatgaatg cgggcggcaa gaaagccgtc ctctggggta cggccctgga caacctggtg
     1321 tcgtggcgga tggtgacgcc gcaggcgcag tggctggaaa tcacgcgcct ggatcacaac
     1381 ctgggcaaga tccacgacgc tgagatggcc ggcttcgagc tgcgctattt cgaagccgac
     1441 ggcaagacgc cggtgcgcac cgagcggctg gacattccgg gccgcacttt ccgcaaggaa
     1501 ggcctgggca aggacgtcac ggacaagttc ctgtcgggcc tgcccggcat ccagaaggaa
     1561 ggctgcgacg gcctgatcac cagtgcgcgc tgggtggtgc atcgcatgcc gcagcacacg
     1621 cgcaccgtgt gcctggaatt cttcggcaat gcccgggacg cggtgcccag catcgtcgag
     1681 atcaaggact tcatgttcgc cgaacaatcg aggtcagggg tgctgctggc ggggctggaa
     1741 cacctggacg accgctacct gcgggcggtg ggctattcga ccaagtccaa gcgcggcacg
     1801 gacgccaaag gcggcccttc gtccgcattg cccaagatgg tcctggtcgg cgacatcgcc
     1861 ggggataacg ccgacgatgt ggcccgggcc acttcggaag tcgtgcgcat cgccaactcg
     1921 cgcagcggcg aggggttcgt cgcgatcagt ccggaggcgc gcaagaaatt ctggctggac
     1981 cgcaagcgta ccgcggccat cagcaagcac acgaacgcgt tcaagatcaa cgaggacgtg
     2041 gtgattccgc tgccgcgcat ggccgaatac accgacggca tcgagcgcat caacatcgag
     2101 ctgtcgctgg ccaacaagat cgctctggcc gaccagctcg aggcgttctt cgccggcggc
     2161 aacctgcccc tgggcaggca tgacgatgcc ggcgagattc cttcggccga attgctggaa
     2221 gaccgcgtag cgcaggcact ggagctggtg cggggcgtgc gcgtgctgtg gcgcggctgg
     2281 ctgcaggacg tcgagaagct gttcccgcaa ttgcaggacc acacgctgcg cgcgagctgg
     2341 aagacccaga tccgcgcgcc gctgcagaac atctttgccg ggcacgcgtt cgggcccatc
     2401 ctggaggaag ccaacgccat ccacaagcgc gtgctcaagg gccgggtctg ggtggcgctg
     2461 cacatgcacg cgggcgacgg caacgtgcac accaacatcc cggtcaacag cgacgactac
     2521 gagatgctgc agaccgcgca ccgggcggtg aaacggatca tggtgcttgc gcgctcgctc
     2581 aacggcgtga tctcgggcga gcacggcatc ggcatcacca agctcgaatt cctcaccgac
     2641 gaggaactcc agccgttcgc cgactacaag cagcaggtgg accccgaggg ccgcttcaac
     2701 aagggcaagc tgctgcgcag accctcaccc ccaccctctc ccgcaagcgg gagagggggc
     2761 caatcactcc cgcctgcgag tggaggccaa tcgttcccgc ctgcgggagc gggccagtca
     2821 ctccctctcc cgcttgcggg agagggccgg ggtgagggca tcatgtacgc cgacctgacc
     2881 aacgcctaca cgccaagctt cggcctgatg ggccatgagt cgctgatcat gcagcagtcg
     2941 gatatcggcg ccatcgccga cagcgtgaag gactgcctgc gctgtggcaa gtgcaagccc
     3001 acgtgctcga cgcacgtgcc gcgcgccaac ctcctgtaca gcccccgcaa caagatcctg
     3061 gctacttcgc tgctggtgga agccttcctg tatgaagagc agacccgccg cggggtttcg
     3121 gtcaagcatt ggcaggagtt cgaggacgtg gccgatcact gcaccgtctg ccacaagtgc
     3181 gagccgccgt gcccagtgaa catcgacttc ggcgacgtgt cgatgaacat gcgcaacctg
     3241 ctgcgcaaga tgggccagaa gagtttccgg cccggcaacg cggccgccat gctgatgctc
     3301 aatgccacca gccccgagac catcaagctg ctgcgcggcg cggtggtcgg catcggcttc
     3361 aaggcacagc ggctggccaa cgacttgctg cgagggctgg cccgcaaaca ggccacccgc
     3421 cccccctcca cgcacgaggc cgcgccggtc aaagagcaga tcatccactt cgtgaacaag
     3481 aagctgccgg gcgggctgcc gaagaagacc gcgcgcgcct tgctggatat cgaggacaag
     3541 gactatgtcc cgatcatccg caatcccaag gcgacgaccg cggagacgga ggcggtgttc
     3601 tattttcccg gctgcggctc cgagcggctg ttctcgcagg tcggcctggc cacgcaggcc
     3661 atgctgtggc atgcgggtgt gcagacggtg ctgccgccgg gttacctgtg ctgcggctat
     3721 ccacagcgcg gctcgggcca gttcgacaag gccgagaaaa tcatcaccga caaccgcgtg
     3781 ctgttccacc gggtcgccaa cacgctcaac tacctggaca tcaagacggt ggtcgtgagc
     3841 tgcggcacct gctacgacca gctgcagggc tacgaattcg agaagatctt cccgggctcg
     3901 cgcatcatcg acatccacga gtacctgctg gaaaagaaca tcacgctccc cgcggggcag
     3961 ggcggcttca tgtaccacga accctgccac aacccgatga agctgcagga ctcgctcaag
     4021 accgtcaagg cgctggtggg cgacaaggtg cgcaagtccg agcgctgctg cggcgagtcg
     4081 ggcacgttcg gggtgagccg tcccgacatc gcgacccagg tgcgctttcg caaggaggaa
     4141 gagctgcgca aggacgaggc ggcgctccgc gaagcgatga aaggagccgc gggcggcgcg
     4201 ggcgcggtag ccgaaaaggg caacctcaag atcctcacca gctgccccag ctgcctgcaa
     4261 ggcctcacgc gctacggcaa cgacctgcag aacggcctgc tggaagccga ctacatcgtg
     4321 gtggaaatgg ccaggcagat cctgggcgac aactggatgc ccgagtacgt tcacgcggcc
     4381 aacagcggcg gcatcgagcg cgtgctggtg tgaaagtagc cggctgcccc ttgtgcgacg
     4441 gacccggcgg cgtggccgtt ttcgagggtc cgaaattccg cgtggtgcgc gccgaagaag
     4501 cgggctttcc cgcgttctac cggctggtgt ggtccgacca tgcgagcgaa ttcaccgatc
     4561 tgtccgccgc cgaccggcgg ttgtgcatgg aggcggtcgc ccaagtggaa caggcgctgc
     4621 gcgcccacct tcagccggcc aagatcaacc tggccgcgtt gggcaacgcc gtgccccatc
     4681 tgcactggca cattgtggcg cgctacgaat gggacagcca tttcccggcg gcggtgtggg
     4741 cggcggcgca acgcgaagtg ccccccggcc ggctcgcggc cgtcgagcag ctgcggcccg
     4801 acctggaaaa ggacctggca ggccggctcg ccaacaagac tggaaatcgc acggcatcgt
     4861 gaaatccgca cgttgtctgc gcgctaccgg cgcgcgcgga cgcgggccgc cgtcacaacc
     4921 cgtgtataac cccacgcagg agaggtgttc agaggaaaga agggtttccg gatggttcaa
     4981 gccatggtcc ccccacaagt tcgacgatcg cgcatggcac gtggcgccct gggcatttgc
     5041 ctgttgctgg ccgccgccgc agccagcgcg caaccggcag gcgaagtcga gttttcccgg
     5101 ggtgtgggct tcgcgcagtc gcccggccag gcgccgcgca cgctgggcaa gggcctgccg
     5161 ctggcggaag gcgacaggtt gaccacggcc gagggcgcat cggccatcat caagcttcag
     5221 gacggcaccc gcatgacggt gcggccgaat tccgagctgt tgctgcaaca gtaccgcttc
     5281 aaggaaaacg cgcccgacaa caacatgctg atgcaactgc tgcggggcgg atttcgcgcg
     5341 gtgacgggcc tcatttcgaa gaacgcaccc aacgccgcca ggatacaaac gagcacggcc
     5401 acgatcggca tccgtggtac cgacttcgat gcgcggctgt gcacccgcga ctgcggcgcc
     5461 gagtcggccc gcgtcacagc atcggcgcgc ccgaacgcgg tgctggccag cgccagggtc
     5521 gtgtcctcga ggggcgaggt caacgccgtc gacggttcgg gtcagcgccg ccgtctggtg
     5581 gacggcggca gcgtctatcc gggcgacctg gtcgagaccg gcagtgccac tcaggccgtg
     5641 ctggcgttcc gggacgacac gcgcatcacg ctgggcgcca ccacgcgctt tcgagtcgac
     5701 aacttcgtct acgacgaccg caacgcgggg gagggccgat tcctggtgtc gctgctcaag
     5761 gggtcggtgc gcgccctcac cggcttgatc gccaaggcca acaagcgcaa cgtcggcttc
     5821 agtaccgcca ccgcgacgat cggcatccgc ggcaccggcc tggatatcaa ttgcaccggc
     5881 gcctgcgcgg gtgaacccgg ggactccgac tccggcctca cgctcttcgc ctggctggga
     5941 tccatcgttg tgacgccgac cggccagagc gccctggagg cgctccaggc cggccagggg
     6001 ctgttcatct ctcccagcgg catccgcacg atcaacaacc aacccgatat ggacggcccg
     6061 cgtcccgaca ccgtcacggt gccgcccaag ttgttttcga acgacaacgt ttcggacgac
     6121 gaggaaggac tgttcgtctt cgttcgcgat ggccacatcg aaatcgcggt ggccggcgaa
     6181 acactccacc tgggccgggg tgaggcgggt ttcgcgggcc acgccggcga cgcgaggcgg
     6241 cccgtgaaca ttccgaagtt catcgacttc gaccagctgc cgctgcccac cgcccgcaat
     6301 ccgctgctgg tcaccgtgtt atcggagtcc ggcatccggc ccaacaacca gtgcaggtag
     6361 gcaaggcagt cgctccgatg acacaattcg tgctcatccg gacttgcacg agaggggcga
     6421 aaaacaatat ggtctcagga tatggcgcgc cccgtccgcg caacgggcgc gcccggctcg
     6481 gcctgctcgc atgggcggcg atgggattga gtgccccggc tttggcccag gcgccggcca
     6541 atcctcccat ggcggccttg gcgccatcgc ccgtcttcac catccggggc ttcaaggtca
     6601 ccggtgagaa cccactgggt gacggcgaga cggcccgcgt cctggcgccg ttcctgcgct
     6661 ccggcgcgac cctgcaaacg ctgcagaaag ccaccgcggc gctggagacg gcactgcgcg
     6721 agcgaggcta cggcctgcat cgcgtggccc tgccgccgca ggaggtcggc gacacggtga
     6781 cgctgaccat cgtcaagttc agcgtcgcga aggtccacat cgaaggccgc agcatttacg
     6841 acgaagccaa cgtgcgccgc gccttgcccg aactgcgcga gggcgacagc cccaatttca
     6901 agcggctggc catccagacg gccatcgcca acgagaaccc caacaagcag gtccaggtgg
     6961 gactgcggga gtccgaggag ccggacaaga tcgatgccac catcacggtg aaggaaagcc
     7021 gaccctggaa cttcgccctg ggcctgtcca acgccggctc gaggtcgacg gggcgcgacc
     7081 ggctgaccgt ctcgggcgcc cacaccaacc tgttcaacct ggaccaccag ttcatcgggg
     7141 cctacaccac ctccctcgag cgaccgcagg acgtgaggca actgggcctg tcgtacaagg
     7201 tgccgctgta cggcctgggc ggcgtcgccg gggccgccta tacgcgctcg gacgtggtgg
     7261 gcgacttcgg tacgttcacc agcaccgggg ccggccatac cttcggcgcc agctacacgc
     7321 tgtacttgcc gccggccggt ggccgccgca gctatgtcct gctgggcctg gaagacaagg
     7381 tcttcgacgc taccgtgatc aacgacaccg tggtcggagc cgaccggcgc agccggccgg
     7441 tgaccctggg ctactcggcg cgcaccggat cggatgcggc gagctggggc tacaacctgg
     7501 acttcgccgc caatacgggg agcggccgca ataacgacct ggcgtcctac cgcagcgagg
     7561 atccgcgcat cacgaccgtg cactggaagg cgctgcgcgg cggggtgagc tactcggcgg
     7621 ctttcgcgaa gacctggacc tgggcggcgc gcggccagtt ccagtacagc cccgacgtgc
     7681 tgatctcggg cgaacagttc ggcctgggcg gcctgggatc ggtgcgtggc accgacatcg
     7741 agcgccccgt caccggcgac aagggcttgt ccgccaccgc ggaagtcggc acgcccgagc
     7801 tgaagccggg cctgcgcctg ctcggtttcc tggacgcggg gtggctgggc aacaacagcc
     7861 ccaacggcac ccacaagccg ggcacggacc gcctggcgag cgttggcctg ggcctgcgct
     7921 acgccaggga gccgttcgcg gtctcgctgg actatggccg cctggtcgtc ggcagccgcg
     7981 tgccgctggc cttcaacgcc gcggcaccgc aaaaaggcga cgacagggtc tatctgaacc
     8041 tgtccgtgcg tttctagcgg gtgacataaa ctcgcgcaat ggcaggttta aattccgata
     8101 gtccgacccc gcgttcgctg actctgcacg ggcagtcgcg cgtactcgag gtctcctttg
     8161 ccgacggcgc gcagttccgg cttccgttcg agctgatgcg cgtctactcc ccttccgcgg
     8221 atgtggcggg ccatggcccg ggccaggagg tgctgcaaac cggcaagcgc gatgtggaga
     8281 tcacgggcct ggagccggta ggcaactatg cggtccagcc cactttctcg gacggccacg
     8341 acaccggtat ctattcctgg gactaccttt atttcctggg ccaacagcag gaacagctgt
     8401 gggccgatta cgagcgtcgc ttgaaacagg ccggcgtcga ccgcgacgcc cccatgcccg
     8461 acaaggcggc ccccagctgc ggccattgac gcgaccggcc cacggattcc ctgtttcagt
     8521 gcaaggttgc acgcgcttgc acgcgcgcgg cgcgcgtttg ccctacgatc acccctatga
     8581 gcagcaccca tttcggtttc gaatccgtgg atgagcgcga aaaggcgcgc cgcgtccgtg
     8641 gcgtgttcga ttcggtcgcg ccccgctacg acctgatgaa cgacctcatg tccatgggct
     8701 tgcaccgtgc ctggaaggct tacacggtgc tggcggccca tgtgcggccg ggcgaccacg
     8761 tgctcgatat tgccgggggc acgggcgacc tgtcgctggc cttcgccagg caggtgggcg
     8821 ccaccggccg ggtcgttcac accgacatca acgaggccat gctgcgcacc gggcgcgatc
     8881 gcctgctgga cgcgggcgtt tccctgccca cgctggtgtg cgatgcggaa aagctgccct
     8941 tcaccccgga cagtttcgac cttgtcagcg tggctttcgg gcttcgcaac atgacgcaca
     9001 aggatgcggc gctggccgag atgaaccggg tgctcaagcc cggcggcaag ctgctggtcc
     9061 tggagttttc caagatcgcc aagccgctgg aaaaggcgta cgactggtac tcgttcaagg
     9121 tgctgccccg gctgggcaag ctggtggccg gcgacgacgc gagctaccgc tacctggccg
     9181 agtcgatccg ggtgcacccg ggccaggagg agctgaaagc gctgatgaag aaatgtggtt
     9241 tcggccatgt ggactatcac aacctgaccg gcggggtcgt ggccctgcac gttggaatca
     9301 agtgttgagg cgcactagcg ccatggaggc agtatgaagt tcttgtctat ggtattcgtg
     9361 gtgctgctgg cgtttgccag cttcgactcg gaggctcgcc gcatgggcgg cggcggatcg
     9421 ttcggccggc aatcgggcaa cgtgacgcaa cgccaggccg gccaaccgcc ggcctcgcct
     9481 gcgcagaacc agcaggcggc accggccaat cagccgggcg ccggggccaa tgcggccgcg
     9541 gccgcgccca ggcgaccctt tggtggaatg ctcggcggcc tggcagcggg ccttggcctg
     9601 gcatggctgg cgcattccct gggcttgggc gaggccttcg cccagttcat gctgatcgcg
     9661 ctggcggtga tggtcgccat ggtggtcatc gggctggtca tgcgctctcg cggcgcgggg
     9721 cagacgggcg gccgctacaa cctgcagagc gccggcgcgc cggcggatct ggcgcaggcg
     9781 ccgcggcaat acagcccgga caaggtcggc aacgatgcgt cggcccgccc ctgggagcgc
     9841 ggcagcatgg cgttcgaagc cgccaagtac ggccagggca gcggcgtgca gatcggctcg
     9901 ggcctcgccg gaccacagtc gtggggtgtc ccgcccggct tcgacaccga aggttttatc
     9961 actgcggcca agcgcaattt cgagagcctg caagatgcct gggaccgctc cgacatctcg
    10021 agcttgcgcg cgatgatgac ggacgagatg ctgggagaga tcaagtccca gttggctgac
    10081 cgcgagaagg accaggccgg caagcccaac aagaccgagg tggtcatgct cgatgcccag
    10141 ctcctgggaa tcgaggacac gggttccgat tacatggcca gtgtggaatt ctccgggatg
    10201 atccgtgagg agccgtcggc cggtcccagc cccttccggg aggtctggaa catgaccaag
    10261 tccaagagcg gcggcagcgg ctggctggtg gcgggcgtgc aggcactgca gtaggttgag
    10321 cgttgggcga tgagggttgg gcgtggaacc cgctcaacgc cccacgctga acgccgaacg
    10381 tcatcgggaa taatgggggg ctatggcaac acagtccccc tttccatttc tggacacctt
    10441 gttcgagcgg ctggcaggag gcctgcagcc gccggcctgg atggtcgagg aagtccagcg
    10501 ccgcctggtg ctccttttca accatgtgct gatgcaggag cccgaggcgc aggcgcggct
    10561 ggcacggcag gccaaccgcg tggtggaagc gcgctggcgc atcttcgtga tgcggctggt
    10621 ggccacgccg gcggggctgc tggacctggc accgggcgcc gcccagcccg acctgacgtt
    10681 gacgctgacc gaggagtctc cgtgggagct ggcgcaggcg gcgctgcgcg gcgataaccc
    10741 gccggtgcgt atcgcgggcg atgtgcagtt cgccaacgaa atcaactggc tggtggagca
    10801 cgtgcgctgg gacctcgagg aagacctctc gcgcctggtc ggcgatgcgc cggcccatgc
    10861 catgggaacc gcggcgcgcc ggatcgtgca ggccttgcgg cagttcacgc gtccctcgcc
    10921 gggttcagga cagccccctg ctcccgggcc tgggcccggg caggcagggg catgacgcgt
    10981 tacctgcgcg gcatttacat tgtctgggtc gcgctgagct acggcctgga cgagctcgtc
    11041 ctgaacagct tccagcggcc gtggctgagt cggatcgcac gcgtcctgtc gttcgggcgc
    11101 aacctgaagg cgccgcgcgg ccagcgcctc aggcaggcac tggaacacct cggcccgatc
    11161 ttcgtgaagt tcggccaggt gctgtccacg cggcgcgacc tgctgcccgc cgacgtcgcc
    11221 gacgaactgg cgcgcctgca ggaccgggtg ccgccgttcg attccgccgt ggccatggcc
    11281 accgtcgagc gcgccttcgg ccggccggtg gacaccatct tcgccacgtt cgagcgcgaa
    11341 ccggtggcca gcgcctcgat cgcccaggtg cacttcgcca cgctgaagga ccgcaacgga
    11401 cagacccgcg acgtggccgt gaaggttctc aggccgggca tgctgaccgt gatcgagaag
    11461 gacctgagcc tgatgaggat gatggccggc tggctggacc gcctgtccaa ggaggcccgg
    11521 cgcctgaagc cgcgcgaggt ggtggcggag ttcagcaagt acctgcatga cgagctggac
    11581 ctggtgcgcg aggccgccgc cgcggcccag ctgcgccgca acatggcggg gctgaacctg
    11641 gtgctgatcc cggagatgct ctgggattac tgccatcccg aggtcatcgt gatggagcgc
    11701 atggtcgggg tgccgatcag ccaggtggag cggctgcgcc aggcgggcgt gaacatcccc
    11761 caactggcgc gcgatggcgt caccatcttc ttcacccagg tgtttcgcga cggctttttt
    11821 cacgccgaca tgcaccccgg caacattcag gtgagcattg agccggcgac cttcgggcgg
    11881 tacgtgtcgc tggatttcgg catcatcggc accctcaccg agtacgacaa ggaatacctg
    11941 gcgcagaact tcacggcctt cttccggcgc gactacaaac gggtggcgca actgcacatc
    12001 gaatccggct gggtgccgcc cgatacccgc gtcgacgagc tggagtcggc ggtgcgcacg
    12061 gtctgcgaac cgtacttcga ccgtccgctc aaggaaatct cgctcggcct gctgttgatg
    12121 cgcctgttcc agacgtcgcg ccgtttccac gtggaaatcc agccgcagct ggtgctgttg
    12181 cagaagacgc tgctgaacat cgaaggcctg ggccggcagc tcgaccccga cctcgacctg
    12241 tggagcacgg ccaaaccgtt cctcgagaaa tggatgctgg agcaggtggg gccgcaaaaa
    12301 ttgctggacg agttgcgcga ccaggcgcca cgctacgcca agctgctgcc cgacctgccg
    12361 ctgctgctgc accagtacct cacgcaaaag ccggtcgacg ggcggcggga aatgctggag
    12421 ctgctggccg aacaga
//
LOCUS       DASWIY010000169         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_836180, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000169 DASWIY010000000
VERSION     DASWIY010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836180"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VGA59_06530"
     CDS             <1..362
                     /locus_tag="VGA59_06530"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEX9719372.1"
                     /translation="RAGRDRDDAQQPGAGLSRGKPGCRAISPTDRRMGSCPTAEVRRF
                     FYHGSSSLQQEAFMSGEDIKVTDTPRTSERAGASQEREMLEWAKTLRISPAQLKQAVS
                     AVGITPRKIREYLRRNH"
     gene            complement(412..642)
                     /locus_tag="VGA59_06535"
     CDS             complement(412..642)
                     /locus_tag="VGA59_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719373.1"
                     /translation="MTEFFSLFEKWVTADRAATQAEYRLSHLLDLYCEGIGPAPALAV
                     IAEARELRAVANARLRSLWKLAHEARACVPVI"
     gene            862..1500
                     /locus_tag="VGA59_06540"
     CDS             862..1500
                     /locus_tag="VGA59_06540"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9719374.1"
                     /translation="MSSSPPTPFADPAGTWNRRFEADGGYLFGTEPNAWLREHAGVWR
                     PGQRVLCVADGEGRNSVWLARQGLAVDAFDIAEAGVAKARRLAAEHGVQVDFAVADCD
                     GLAWPEGIYDGVAAIFVQFADPPMRERLFANIQRCLKPGGILVLQGYTPKQLEYRTGG
                     PPIASHLYTQALLRDAFADMEILELQEYEAEVAEGSGHRGWSALIGMVARRR"
BASE COUNT          341 a          610 c          621 g          300 t
ORIGIN      
        1 gtcgcgccgg acgtgatcgc gacgacgctc agcagcctgg cgccggcttg agtcgcggca
       61 agcccggatg tcgggcgatc agcccaaccg acaggcggat gggcagttgt cctacagccg
      121 aggtgcggcg gtttttctac catggcagct cttcactgca gcaggaggca ttcatgtcgg
      181 gcgaggacat caaggtcacg gacacgccga gaaccagtga acgggccggc gcgagccagg
      241 aacgcgagat gctggaatgg gcgaaaaccc tgcgcatctc gccggcgcaa ctcaaacagg
      301 ccgtatcggc ggtggggatc acgccaagaa aaatccgcga gtacctgagg cgcaaccact
      361 agccgggccg ggtaaacccg aaggcctgcg tcttcttccc cgatccgctg cctagatcac
      421 cggcacgcac gcccttgctt cgtgcgcaag cttccaaagc gagcgcaggc gggcgttggc
      481 gacggcacgc agttccctgg cctcggcaat gaccgccagc gccggtgcgg gaccgatgcc
      541 ctcgcaatac aggtcgagca gatggctgag gcggtactcg gcctgggtgg ccgcacggtc
      601 ggcggtcacc catttctcga acagcgaaaa gaactccgtc atggcagtca gtcaatgaat
      661 cttgcaccac gattaaaagg cagcatgggc ggggccgtat ttgagcaaga tcagacgatc
      721 tgcgttgcag cttgtccttc cgcgatcgac tgaggcagca caagtgcaat agtgcgcgcc
      781 cgcggtcgtc gccccgacag cggcacgggg cggcgccatc ggcgcggcga aacggcctat
      841 cctttcgtta cgatatgcgc catgagctcc tcccccccga caccgttcgc cgaccccgcg
      901 ggaacctgga accgccgttt cgaagccgac ggcggctatt tgttcggcac ggaaccgaat
      961 gcatggctgc gcgagcatgc cggcgtgtgg cgccccggcc agcgcgtgct gtgcgttgcc
     1021 gacggcgaag gccgcaacag cgtctggctg gcccggcaag ggctggccgt cgatgccttc
     1081 gacatcgccg aggccggcgt ggccaaggcg cggcgcctcg cggcagagca cggcgtccag
     1141 gtcgacttcg ccgtggccga ttgcgatggc ctcgcatggc ccgagggtat ctacgacgga
     1201 gtcgcggcca tcttcgtgca gtttgccgat ccgcccatgc gtgagcgcct gttcgcaaac
     1261 atccagcgct gtctcaagcc cggtggcatc ctggtgctgc aaggctatac gcccaagcag
     1321 ctcgaataca ggaccggcgg ccctccgatc gcatcgcacc tgtacacgca agccctgctg
     1381 cgcgatgcgt tcgccgacat ggaaatcctg gagctgcagg aatacgaggc cgaagtggcc
     1441 gagggcagcg ggcatcgcgg ctggtcggcg ctgatcggaa tggtggcgcg gcgccggtga
     1501 gagctgaaga cgctgtcatt gcggggtggg ctcgcggaac gcccggggcg cgcagctgtc
     1561 cagtgcagcg agcccaatgc cgggcctgca ctatgcacgt gccgaggcat cacgctccgg
     1621 cccggcgctc gcggaacgcc cggggcgagc atttgggcca atgccacgct gagggacaag
     1681 actaaggtct tcaagtcccg atacaagaca cacccctcgc cggaggccgg aaccctcacg
     1741 agggcgattg ctgccgtttg tattccccgc gcttcatcgt gaggtagacc tcacggtcga
     1801 gttcatgcag ctgcctgggg tacttctcgg tcgccgcgaa ccagagcgcg atccttctct
     1861 cgaactgctc cg
//
LOCUS       DASWIY010000170         5397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1582996, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000170 DASWIY010000000
VERSION     DASWIY010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582996"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGA59_06545"
     CDS             <1..240
                     /locus_tag="VGA59_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719375.1"
                     /translation="GGYVSTPAKVLILIATAPIVVLLAPIWLLGVLAGGSGAGEQAQT
                     SGQSKPTDRPSKDCCFIWLVHGSTGEVVAGTAPRQ"
     gene            578..2581
                     /locus_tag="VGA59_06550"
     CDS             578..2581
                     /locus_tag="VGA59_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9719376.1"
                     /translation="MIQRVAVKLSLLAMALLIPATGIYSLAHLQKQAATAEQVKGMTV
                     AGDAGEGVWTLGDSPLVVFGTGSVLGFLETHPVERITVIYKPLTWNVSERIILVESRG
                     RQHAYYPSDMESKIFADKAVTAPWGGKLAFVARGELGPEGLELLERLDQRFASARPQS
                     EKDRWIALASGGLSAVLMAGLLGFLFLQFKGQFKSLKFIEPAQVQGSIDDLVGMDDIK
                     SEVAQIKDQYQRRAAYAEYGISKPFNVMFSGPAGTGKTKLASYLAKELRLPILFHSAA
                     NLETGFVSGGSNTLSRIIAMARRRKRCIVFLDEAQDLFMKRGGRRKFDDDTANTLLSV
                     LDGVRSRNEAEIIWIVASNFNSETMKMDEAMLRRFQMKVDFRMPNREERLAIFGHYLD
                     SRVGKVQPGLDLRPLVAVTEGRSPADLETIVNQAGITAVQAGEIIGADTLMRAAERVL
                     VGNVNSHTTQERERDRRIIAVHEWGHFLVDLEHERKLANGDWARILAEMKTIKISLKA
                     NPRTNALGFVFHKQGVNLLKTKGDIEHDVRVLLGGMANEELFFGEEGTTNGAHNDITR
                     VTQLLHHAVGEMGMYRSTRLNFGALARDGERGGREVDKETRGIMEAQSERLYGETKAV
                     LLRLKLLTEHLVGKLLAAGEMSLNEALVEIRSFEDSNLGADRQ"
     gene            3011..3247
                     /locus_tag="VGA59_06555"
     CDS             3011..3247
                     /locus_tag="VGA59_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719377.1"
                     /translation="MAIVTVRIPESLLKRAQATTGRRDAASAVRRALEIATYDYELNA
                     AARRALGQSRREEREGKVRRFTTARDAVAHLKRL"
     gene            3244..3567
                     /locus_tag="VGA59_06560"
     CDS             3244..3567
                     /locus_tag="VGA59_06560"
                     /inference="COORDINATES: protein motif:HMM:NF027074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEX9719378.1"
                     /translation="MKEIVFTRRFERDFRRLKKRLSSYVLDYETLECVFELLQRGKSL
                     PEAFREHGLEGEYAGYTECHLDADWLLVYRAREEIRIALRLIQRTRKTLGFETPAAKL
                     QAVLH"
     gene            complement(3613..3873)
                     /locus_tag="VGA59_06565"
     CDS             complement(3613..3873)
                     /locus_tag="VGA59_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719379.1"
                     /translation="MKAKKLKAERGKESSTAYFQNLRPKFLDWNPPTEEEKALEVTAR
                     RQAREFELKRVTELAKRREAEKADMTKRNRIAGARKKQRKRG"
     gene            4114..5106
                     /locus_tag="VGA59_06570"
     CDS             4114..5106
                     /locus_tag="VGA59_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX9719380.1"
                     /translation="MNKRIVLASYPEGWVTDTNFRLETAPIPKPAEGEVLVKNLWLSL
                     DPYMRGRMSQQKSYVKGVEPGEVMTGETAGEVVASKHPKFKPGDKVTAPAGWQLYWAG
                     KGEMLTKVDASKVSLSYFLGCLGVPGRTAYFGMKDICPPKSGETVLVSAASGAVGSVV
                     GQLAKAWGCRAVGIAGGKAKCDYTVKELGFDACVDYKAGDLYGQLKAACPKGVDALFE
                     NVGGEILDTALRLMNVRGRIAICGLISDYNAKEPYGVKMFRSILVNRLTVRGMIVFDW
                     LERYPEANQALIEMVLGGKLKYRESILEGIENAPKGLIGLLKGDNFGKQLVKLT"
     gene            5103..>5397
                     /locus_tag="VGA59_06575"
     CDS             5103..>5397
                     /locus_tag="VGA59_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9719381.1"
                     /translation="MIDLYTWPTPNGHKIHIMLEETGLPYKVIPIDIGAGDQFKPEFL
                     KISPNNKMPAMVDSDGPDGKPMALAESGAMLFYLASKTGKFLPENMRKRWQVMQ"
BASE COUNT         1023 a         1699 c         1748 g          927 t
ORIGIN      
        1 ggcggatatg tcagcacgcc cgcaaaggtt ctgatactga tcgccacggc cccaatagtc
       61 gtgttgctcg cgccgatatg gctgttgggt gtgctcgccg gaggttccgg agctggagag
      121 caagctcaaa cttcggggca aagcaagccg accgacaggc cgtcgaagga ctgctgcttc
      181 atttggcttg tacacggctc gaccggcgag gtggttgccg gaaccgcgcc acggcagtag
      241 ctgcgcgaac gttgccgcga aggctgggtg accgaggccg atttccgcgt cgaaaccgcg
      301 ctggcgcgca agcctccctg ccaataggac gcgattaagc gcgctggcgt accgagcgcc
      361 tgggccggga aggctacgat gccccaaccc actcgaaagg aaatcccatg aatggcatcg
      421 tctacctggt cggcgcaatc gtcatcatcg tcgccctcct gtcgttcctc gggctgcgct
      481 gacgcgcctg aaagcgaaaa cccgagacgc ggcgccggcc tcgccggggc tgtggttcgc
      541 catccgcaag tcggccagcc gactgcagga ctggttcgtg attcagcggg tggccgtcaa
      601 gctgagcttg ctggccatgg ctctcctgat cccggccacc ggcatttact cgctcgcgca
      661 cctgcagaag caggccgcaa ccgccgagca ggtcaagggc atgaccgttg ccggggatgc
      721 cggcgaaggg gtgtggacgc tcggcgactc accgctcgtg gtattcggca ccggctcggt
      781 gctgggcttc ctggagaccc acccggtcga gcgcatcacg gtgatctaca agccgctgac
      841 gtggaatgtg tcggagcgca tcatcctggt cgaatcccgc ggccggcagc acgcctacta
      901 ccccagcgac atggagagca agattttcgc cgacaaggcg gtcaccgcgc cctggggcgg
      961 gaagcttgca ttcgtcgcgc gcggcgagct gggccccgag gggctcgagt tgctcgagcg
     1021 cctggaccag cgctttgcca gcgcccgccc gcagtcggag aaggatcgct ggatcgccct
     1081 cgcgagcggc gggctgtcgg ccgtgctgat ggccggcctg ctcggcttcc tgttcctgca
     1141 gttcaagggc cagttcaagt cgctcaagtt catcgagccg gcgcaggtgc aaggctccat
     1201 cgacgatctg gtgggcatgg acgacatcaa gtccgaggtg gcgcagatca aggaccagta
     1261 ccagcgtcgt gcggcgtatg ccgagtacgg catcagcaag cccttcaacg tgatgttcag
     1321 cggcccggcg ggcaccggca agaccaagct ggccagctac ctggccaagg agttgcggct
     1381 gccgatcctg ttccactccg ccgccaacct ggaaacgggt ttcgtgtcgg gcggctccaa
     1441 cacgctcagc cgcatcatcg ccatggccag gcgccgcaag cgctgcatcg tgtttctgga
     1501 cgaggcgcag gacctgttca tgaagcgcgg cggccgccgc aagttcgacg acgacaccgc
     1561 caacaccctc ctgtccgtgc tggacggcgt gcgcagcagg aacgaggccg agatcatctg
     1621 gatcgtcgcc agcaacttca acagcgaaac catgaagatg gacgaggcca tgctgcgccg
     1681 cttccagatg aaggtggact tccgcatgcc caaccgggaa gagcggctgg ccattttcgg
     1741 ccactacctg gatagccgcg tcggcaaggt gcaacccggc ctggatctgc gccccctggt
     1801 ggcagtgacc gagggccgca gcccggcgga cctcgagacc atcgtcaacc aggccggcat
     1861 cacggccgtg caggccggcg agatcatcgg cgccgatacg ctgatgcggg ccgccgagcg
     1921 cgtgctggtg ggcaacgtca acagccacac cacccaggaa cgcgagcgcg accgacgcat
     1981 catcgccgtg cacgaatggg gccacttcct ggtggacctc gagcacgaac gcaagctggc
     2041 caacggcgac tgggcgcgga tcctggccga gatgaagacc atcaagatat cgctcaaggc
     2101 caacccgcgc accaacgcgt tgggcttcgt cttccacaag cagggggtca acctgctcaa
     2161 gaccaagggc gacatcgaac acgacgtgcg tgtgctgctg ggcggcatgg ccaacgagga
     2221 gctgttcttc ggcgaggagg gcacgaccaa cggggcgcac aacgacatca cccgggtgac
     2281 gcagctgctg caccacgccg tgggcgagat gggcatgtac cgcagcaccc gcctcaactt
     2341 cggtgccctc gcccgtgacg gcgaacgcgg cggccgcgaa gtggacaagg agacgcgcgg
     2401 catcatggaa gcgcagagcg agcgcctgta cggcgagacc aaggccgtgc tgctccggct
     2461 caagctgctg accgagcacc tggtcggcaa gctcctggcg gccggcgaaa tgagcctgaa
     2521 cgaggcgcta gtcgagattc gcagcttcga ggattcaaac ctgggcgccg accggcaata
     2581 ggcgcaggct ttcctggcag cagggacagc cggagggaca tcgcgggggg acggtaggca
     2641 gggggcgggg tgctgtcgac cgatggtgcg ggtgtggcca gaacgctatt cacaatcagc
     2701 gtcttagcat catagtcttg tagccaaaaa cagaagactc ttgtattcag aacacgcagg
     2761 tgacgtctaa gccgcttcct tgtcgagcgt cttcggaacc aggatctctt tcgagccggc
     2821 ttgccgcgtg gcttttcagc ccgagaacga ataaccgcgc gctgtgcgac acatcggcgt
     2881 tgcggttgcc gaggtcggcg tacacgttgg cgctgccttc gtgaatctcg atgtgtcgaa
     2941 tgaccgcggt ttgctcatgc ctgggcgacg cggctggcgg gctacaatgc gaacgttcaa
     3001 ggagagtgta gtggccatcg taaccgtccg tattccagaa tcgttgctca agcgtgccca
     3061 ggcgaccacg gggcggcgcg acgccgcgtc cgcggtgcgc cgggcgctcg agatcgccac
     3121 gtacgactac gagttgaacg cggcggcgcg gcgcgcgttg gggcagtcgc ggcgcgaaga
     3181 gcgcgaaggc aaggttcgtc gcttcaccac cgcgagggac gccgtcgcgc atctcaagcg
     3241 tttgtgaagg agatcgtctt cacgcggcgc ttcgaacgcg atttccgccg cctgaagaaa
     3301 cgcctctcct cctacgttct cgattacgag acgctggaat gcgttttcga gctgcttcag
     3361 cggggaaagt cactacccga agccttcagg gaacacggcc tcgaaggcga gtacgccgga
     3421 tataccgagt gccatctcga cgcggattgg ctgctggtct accgcgcgcg tgaggagatc
     3481 aggatcgcgt tacgcctcat tcaacgcacc agaaagactt tgggctttga aacccctgcc
     3541 gctaaactac aagcagtgtt gcattgaccg gttgaacccg ccgccaaaag cgggcattgg
     3601 cgcggacctg ccctaaccac gtttgcgttg cttctttctg gcgcctgcga tccggttgcg
     3661 ctttgtcatg tctgctttct ctgcttctct gcgcttggcc agttcggtaa ccctcttcaa
     3721 ctcgaactct cttgcttgcc ttcgtgccgt cacctccagc gccttttctt cctccgtagg
     3781 cggattccag tcgagaaatt tcggcctcag attctgaaaa taggcggtgg aggattcctt
     3841 cccacgctct gctttcaact ttttggcttt catcttggct gcttccggtc aggacgctcc
     3901 ctcatgctgg gatcttcgca tgcagggagg cgaaacccca gattgcgatt tcggctgaca
     3961 ggcgcgcgag ggctatccga acaagctgtc ctgctcctac cgtaccgtac cctggcgcga
     4021 acgactgctt ggggtcgagg cagccactca aaccgagaca cccccgccaa tacctgcgct
     4081 accatcgggt tccgttttcc agggaatccc cgcatgaaca agcgcatcgt cctcgcgagt
     4141 taccctgaag gctgggtaac ggacaccaat ttccgcctcg aaaccgcgcc catccccaaa
     4201 cccgcagagg gcgaggtgct ggtgaagaac ctctggctgt cgctcgaccc gtacatgcgc
     4261 ggccgcatga gccagcagaa gagctacgtg aagggcgtcg agccgggcga ggtgatgacc
     4321 ggcgaaacgg cgggcgaagt ggtcgcatcg aagcacccga agttcaagcc cggcgacaag
     4381 gtgaccgcgc ccgcgggctg gcagctgtac tgggccggca agggggagat gctgacgaag
     4441 gtggacgcct cgaaagtgtc gctgtcgtac tttctcggct gcctcggcgt gccggggcgc
     4501 acggcctact tcggcatgaa ggacatctgc ccgcccaagt ccggcgagac ggtgctggtg
     4561 tccgccgcct ccggcgcggt gggcagcgtg gtgggccaac tcgccaaggc ctggggctgc
     4621 cgcgcggtgg gtatcgcggg cggcaaggcg aagtgcgact acacggtgaa ggaactgggg
     4681 ttcgacgcct gcgtggacta caaggcgggc gacctctacg gacagttgaa ggcagcctgc
     4741 cccaagggcg tggacgcgct gttcgagaac gtcggcggcg agatcctcga cacggcgctg
     4801 cgcctgatga acgtgcgcgg gcgcatcgcc atctgcggcc tgatctccga ctacaacgcc
     4861 aaggagccct acggggtgaa gatgttccgc tcgatcctgg tcaaccgcct cacggtgcgg
     4921 ggcatgatcg tgttcgactg gctggagcgc tacccggaag cgaaccaggc cttgatcgag
     4981 atggtgctcg gcggcaagct gaagtatcgg gaatccatcc tcgaaggcat cgagaacgca
     5041 cccaagggcc tgatcgggct gctcaagggc gacaacttcg gcaaacaact ggtgaaactc
     5101 acatgatcga cctctatacc tggcctacgc ccaacgggca caagatccac atcatgctgg
     5161 aggagacggg cttgccctac aaggtgatcc cgatcgacat cggcgcgggc gaccagttca
     5221 agcccgagtt cctgaagatc tcgcccaaca acaagatgcc ggcgatggtg gacagcgacg
     5281 gcccggacgg caagccgatg gcgctcgccg agtccggcgc catgctgttc tacctcgcct
     5341 cgaagaccgg caagttcctg cccgagaaca tgcgcaagcg ctggcaggtg atgcagt
//
LOCUS       DASWIY010000171         3052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2690516, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000171 DASWIY010000000
VERSION     DASWIY010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2690516"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(545..919)
                     /locus_tag="VGA59_06580"
     CDS             complement(545..919)
                     /locus_tag="VGA59_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719382.1"
                     /translation="MTEGNYFLTLPGRAEPLHIGKSSAGWRFKLHVEPGRGIEGLADW
                     EALWRQRGAAISHAGGAALLAEDMRRIIAERRHGGREWTRERLAMHGGEPGPNGLARH
                     RVGGQCVGHGDGTFDLFEGFFR"
     gene            complement(916..1059)
                     /locus_tag="VGA59_06585"
     CDS             complement(916..1059)
                     /locus_tag="VGA59_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719383.1"
                     /translation="MFEVFTFTIVAAIVAAAALVVFLSIKADRAITADHNEDDDREDD
                     ELQ"
     gene            complement(1272..1568)
                     /locus_tag="VGA59_06590"
     CDS             complement(1272..1568)
                     /locus_tag="VGA59_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719384.1"
                     /translation="MKDHHRACKTCACFEPRNAADTGGTCHRHPQREYVERDYWCMEH
                     RPKADPLFGRDDVERALELDDAERQAGGMFSPVTYRTSGDEWPIAMLRRLTRRL"
     gene            complement(1565..1960)
                     /locus_tag="VGA59_06595"
     CDS             complement(1565..1960)
                     /locus_tag="VGA59_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719385.1"
                     /translation="MILTRRAFFVGTSKAVALASLVVGTPALVLAARLLPTESPPATP
                     LKPFWPIGALARLDRNEQVVIGERYLRAVPLGYAELKPLMSRYMLPPDMQDITPAVDW
                     SVPDIEAHALRTLIEAAREYEFARAGWRP"
     gene            complement(1965..2270)
                     /locus_tag="VGA59_06600"
     CDS             complement(1965..2270)
                     /locus_tag="VGA59_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719386.1"
                     /translation="MRALVILAAVLMLGACASRHAAREHAACLSYGLTPELAGYADCR
                     LALAQQRQAMGLMLMGQGLATLSAANRPVYRPPVFLPPMRLDCTSIAQGAFVNTSCR"
     gene            complement(2273..2965)
                     /locus_tag="VGA59_06605"
     CDS             complement(2273..2965)
                     /locus_tag="VGA59_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEX9719387.1"
                     /translation="MLSAKRARFVAEYLVDSNATQAAIRAGYSPTSAMVTGCRVLKDA
                     KVQAEIAARQLSLIKKLEITQRRVVEELALIGFANMGDYMRATQNGDPYLAFAELTRE
                     QKAALAEVTVEDFVDGRGEEARDVRRVKFKLYDKRAALVDLGRHLGMFPTKGEQNGAG
                     GLNVGVRLIVQIVRGEESVTIDATPPGRGAQDAPGRAEATLVAARTGQDAATHQRAAQ
                     AVLGPSGDQEGA"
BASE COUNT          501 a         1020 c          975 g          556 t
ORIGIN      
        1 tcgttgtgca aggaacgacg gtatctgcgc cttatccgtc ttgccgtcga atacaatccc
       61 gttggccgtg atagagacgc ggatgttggt cccatccacc ttttccgtga aggcccatgc
      121 gttgccggcg agatacgcga actcaggcaa ggctcactcg gatggcagaa gcgtcttgca
      181 agaggtcgcc ggatcacgtt tccacacggt ctgaatcttg tggtattccg tcattgcacg
      241 actcccgtcc ccttacactc gggacacgcg atcatgccgt aacgaatttg aggccggggc
      301 atgttgagcc tatcgtgcgg tacgaatttt gcgccgctgc acagccagca gggggacgga
      361 acgatcgctg gctctttggg cgtggattcg gcgcctatct gacctaccgc ttccttgagc
      421 cgcgctgtat gcgccgcatg cacagcaaca gccgcaacat gctccgcatt gaccgaccca
      481 gcagcccatg cctccaatac tttccttagc gcggcctcta cagcctcaac ctcgtcaacc
      541 tctttcatcg gaaaaacccc tcgaacaaat caaacgtccc atctccgtgc ccgacacact
      601 ggccgccgac ccggtggcgc gccagcccgt tcggcccggg ctcgccaccg tgcatcgcca
      661 gccgctcgcg cgtccactcc cggccgccgt ggcgccgctc ggcgatgatc cggcgcatgt
      721 cctcggccag gagtgcagcg ccgccggcgt gcgatatagc cgcacctcgc tggcgccaga
      781 gcgcttccca gtcggccagt ccctcaatgc cgcgtcctgg ctcgacgtgc agcttgaagc
      841 gccagccggc agaggacttc ccgatgtgca gcggttcggc gcggccgggc agtgtgagga
      901 agtagttgcc ttcagtcatt gcagctcgtc gtcctctcga tcatcatctt cgttgtggtc
      961 ggccgtgatg gcgcggtcgg ccttgatgga caggaacacg accagcgcgg cagcggcaac
     1021 gatggcggca acgatggtga acgtgaagac ctcaaacacg acgtttcact ctcgaacagg
     1081 gagggtagat tcatcgccct gccccggctc ctcgtccact ggcggcggcc cgcggcgcac
     1141 gagccccggc ccaggtacac cgcagttcgg gcagcccatc cgagcgtggc gccggcgccc
     1201 tggcgcgggc tgctgcggca cgatcagata gccgtggtcg ttgcagaccc ggcagtgggc
     1261 gcggcgcttc atcacagcct ccgcgtgagc cggcgcagca tcgcaattgg ccattcgtcg
     1321 ccgctggtcc tgtaggtcac ggggctgaac atcccgccag cttggcgctc cgcatcgtcc
     1381 agctcaagcg cgcgctctac gtcgtcgcgc ccgaacagag gatcagcctt cggccggtgc
     1441 tccatgcacc aataatcccg ctccacgtat tcccgttgcg ggtgccggtg gcacgtcccg
     1501 cccgtgtcag cggcgttgcg cggctcgaag caggcgcagg tcttgcaagc gcggtggtgg
     1561 tctttcatgg gcgccatccg gcgcgggcaa actcgtactc gcgggcagcc tctatcagcg
     1621 tgcgtagggc atgcgcctca atgtcaggga cggaccagtc caccgccggg gtaatgtctt
     1681 gcatgtccgg cgggagcata taacgcgaca tcaacggttt gagctcggca tagccgagcg
     1741 gcacagctcg caggtagcgc tcgccaatca cgacctgctc gttgcgatca agccgagcca
     1801 gcgccccgat cggccagaat ggcttgagcg gcgtcgcggg cgggctctct gtcggcaata
     1861 gcctggccgc cagcaccagc gccggcgtgc cgacgaccag gctcgccagc gcgaccgctt
     1921 tcgatgtgcc gacgaagaat gcgcggcggg ttaagatcat accatcaccg gcagctcgtg
     1981 ttcacgaagg cgccctgcgc gatcgacgtg cagtccaggc gcatcggcgg caggaacacg
     2041 ggtggacggt acaccgggcg gttggcggcc gacagggtag cgagcccctg ccccatgagc
     2101 atgagcccca tggcttggcg ctgctgggcc agcgcgagcc ggcagtcggc atacccggcc
     2161 agctctggcg tcaggccgta ggacaggcag gcggcgtgct cgcgggcggc gtgacgtgag
     2221 gcacaggcac cgagcatcag gacggcggca aggatcacga gggcgcgcat cgtcaggctc
     2281 cttcctggtc gccggatggc cctaggaccg cctgtgctgc ccgctgatgc gtggcggcat
     2341 cttgcccggt cctggctgcc acaagggtag cctccgcgcg tcctggggca tcctgggcgc
     2401 cccgtcccgg cggcgtggca tcgatggtca cagattcctc accgcggacg atctgcacga
     2461 tcaggcggac accgacgttc aggccgcctg cgccgttctg ctcgcccttg gtcgggaaca
     2521 tgccgaggtg gcggcctaga tcgaccaggg cggcgcgctt gtcgtagagc ttgaacttca
     2581 cgcgccgcac gtcacgggcc tcctcgccgc ggccgtcaac gaaatcctcg accgtgacct
     2641 cggcaagcgc ggccttctgc tcgcgggtca gctcggcgaa ggcgaggtac gggtcgccat
     2701 tctgggttgc ccgcatgtag tcgcccatgt tcgcgaagcc gatcagggcg agctcttcga
     2761 ccacgcgccg ttgcgtgatc tcgagttttt taatgaggct tagctgtcgc gctgctattt
     2821 ctgcctggac cttagcgtcc tttagcaccc gacagcctgt caccatcgcc gaggtgggtg
     2881 agtacccggc cctgatcgcg gcctgggtag cgttgctgtc cacgaggtac tcggcaacga
     2941 aacgggcacg cttagcgctc agcatggggg attctctggc acatctggat cggtggtgtc
     3001 aaggcttagc cattttcggg catggaagcg tagttctgca acttccaata gt
//
LOCUS       DASWIY010000172         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3066616, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000172 DASWIY010000000
VERSION     DASWIY010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3066616"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /gene="petA"
                     /locus_tag="VGA59_06610"
     CDS             <1..269
                     /gene="petA"
                     /locus_tag="VGA59_06610"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX9719388.1"
                     /translation="YRSIKPEVWVGVGICTHLGCSPSDKLQAGPQSSMPDDWLGGFFC
                     PCHGSTFDLAGRVFKNKPAPDNLEVPPHMYLSDSRLLIGEDTKG"
     gene            336..1781
                     /locus_tag="VGA59_06615"
     CDS             336..1781
                     /locus_tag="VGA59_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEX9719389.1"
                     /translation="MAEFKEISPNAALGEKLANWFDNRFPSAGSMYRDHMSEYYAPKN
                     FNFWYFFGSLAMLVLVIQIVTGIFLTMHYKPDAAAAFASVEYIMRDVPWGWLIRYMHS
                     TGASAFFVVVYLHMYRGLIYGSYRKPRELVWIFGCAIFLALMAEAFMGYLLPWGQMSY
                     WGAQVIINLFSAIPFIGPDLSEWIRGDYVVGDATLNRFFAFHVIAVPLVLLGLVVAHI
                     LALHDVGSNNPDGIEIKGPNAPLDEKGRPLDGIPFHPYYTIHDIFGVSVFLMVFSAII
                     FFAPEVGGYFLEYNNFIPADPLKTPLHIAPVWYFTPYYSMLRAITTEMMYALSAILVL
                     TVAFVFLKTSASATTKGLLTVLALAFLWMFNAFGFTGAGLLQGIDAKFWGVVVMGGSV
                     VILFFLPWLDKSPVKSIRYRPGWHKWVLGVFIVDFIVLAYLGTQPPTPVGERVSQLGT
                     LFYFGFFLLMPWWSRLGEFKPVPDRITFAAH"
     gene            1796..2554
                     /locus_tag="VGA59_06620"
     CDS             1796..2554
                     /locus_tag="VGA59_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX9719390.1"
                     /translation="MKKLILTLIAALGIATGAQAAGGGGISWDKAPNNLSDLPSLQNG
                     AKLFVNYCLTCHSAAYMRYNRLRDIGLSEQQIKDNLLFTTDRVGDTMAATMNSRQAKE
                     WFGGVPPDLTLVARSRSGPGGSGADYLYTYLRTYYRDPTKATGWNNLVFPDVGMPHVL
                     WELQGEREPLFATRDENGHQVKVFQGWKQVSPGTMTPLQYEQAVGDLVGYLEWMAEPA
                     QNSRVRVGVWVLLFLGILTLFTWRLNAAFWKEIN"
     gene            2674..>2813
                     /locus_tag="VGA59_06625"
     CDS             2674..>2813
                     /locus_tag="VGA59_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX9719391.1"
                     /translation="MMVLYSGTTCPFSHRCRFVLFEKGMDFEIRDVDLYNKPEDINVM
                     NP"
BASE COUNT          483 a          917 c          836 g          577 t
ORIGIN      
        1 aataccgctc gatcaagccc gaagtctggg tgggcgtggg catctgcacg catctgggct
       61 gttcgccgtc cgacaagttg caggccggcc cccagtcgtc gatgccggac gattggctgg
      121 gcggcttctt ctgcccctgc cacggctcga ccttcgatct cgcgggccgc gtattcaaga
      181 acaagcccgc gccggacaac ctggaagtgc cgccgcacat gtacctctcg gacagccgtc
      241 tgctcattgg cgaagacacg aaaggatgag attcttgcgg gacggatcag gattgcccgc
      301 agtgcgcccc cgtctccaac cgatcaagac gattcatggc tgaattcaag gaaatttctc
      361 ccaacgccgc tctgggcgaa aagctcgcca actggttcga caaccgcttt ccttcggcgg
      421 ggtccatgta ccgggaccac atgtcggagt actacgcgcc gaagaacttc aacttctggt
      481 atttcttcgg ctcgctggcc atgctggtgc tggtgatcca gatcgtcacc ggcatcttcc
      541 tgacgatgca ttacaagccg gacgccgccg cggctttcgc ttcggtcgag tacatcatgc
      601 gcgatgtgcc ctggggctgg ctgatccgct acatgcactc caccggcgcc tcggcattct
      661 tcgtcgtggt ctacctgcac atgtaccgcg gcctgattta cggcagctac cgcaagccgc
      721 gcgagctggt ctggatcttc ggctgcgcga ttttcctggc gctgatggcc gaagccttca
      781 tgggctacct gctgccctgg ggccagatgt cctactgggg cgcgcaggtc atcatcaacc
      841 tgttctcggc gattcccttc atcggtccgg acctgtcgga gtggatccgc ggcgactatg
      901 tggtgggcga cgccacgctg aaccgcttct tcgctttcca cgtcatcgcc gtgcccctgg
      961 tgctgctggg cctggtggtc gcgcacatcc tggcgcttca cgacgtcggc tccaacaacc
     1021 ccgacggcat cgagatcaag ggccccaatg cgccgctgga cgaaaagggc cggcccctcg
     1081 acggcatccc tttccatccg tactacacga tccacgacat cttcggcgtt tccgtgttcc
     1141 tgatggtgtt ctccgcgatc attttcttcg cgcccgaagt cggcggctat ttcctcgaat
     1201 acaacaactt catcccggcc gatccgctga agacgccgct gcatatcgcg ccggtctggt
     1261 atttcacgcc ctactactcg atgctgcgcg ccatcacgac cgagatgatg tacgcgctgt
     1321 ccgcgatcct ggtcctcacc gtggccttcg tctttctcaa gacttccgct tcggcgacga
     1381 caaagggcct gctcacggtg ctggcgcttg cgttcctgtg gatgttcaac gccttcggct
     1441 tcacgggagc cggcctgctg cagggcatcg acgcgaagtt ctggggcgtg gtcgtgatgg
     1501 gcggatccgt cgtcatcctg ttcttcctgc cctggctgga caaaagcccg gtcaagtcga
     1561 tacgctaccg gcccggctgg cacaaatggg tgctgggcgt gttcatcgtg gacttcatcg
     1621 tgctggcgta cctgggcacg cagccgccga caccggtcgg cgagcgcgtg tcgcagctgg
     1681 ggacgctttt ctactttggc ttcttcctgc tgatgccgtg gtggagccgg ctgggcgagt
     1741 tcaagcccgt tccggaccgc atcaccttcg ccgcgcactg agccggaccg caagaatgaa
     1801 aaaactcatc ctcaccctga tcgccgcgct gggcatcgcc accggcgcgc aggccgcggg
     1861 cggcgggggc atctcctggg acaaggcgcc gaacaacttg agcgacctgc cgtcgctgca
     1921 aaacggcgcc aagctgttcg tcaactattg cctcacctgc cactcggccg cctacatgcg
     1981 ctacaaccgg ctgcgcgata tcggcctgtc ggagcagcag atcaaggaca acctgttgtt
     2041 cacgaccgac agagtcggcg acaccatggc cgcgaccatg aattcccgcc aggccaagga
     2101 gtggttcggc ggcgttccgc ccgacctgac cctggtcgcg cgttcgcgct cgggcccggg
     2161 cggcagcggc gccgattacc tgtataccta cctgcggacc tactaccgcg acccgaccaa
     2221 ggcgaccggc tggaacaacc tcgtgttccc ggacgtcgga atgccccacg tgctgtggga
     2281 attgcagggc gagcgcgagc cgctgttcgc cacgcgcgac gagaacggcc atcaggtcaa
     2341 ggttttccag ggctggaagc aggtgagccc cggcaccatg acgccgctgc aatacgagca
     2401 ggcggtcggc gacctcgtcg gctacctgga gtggatggcc gagcctgcgc agaattcgcg
     2461 cgtgcgcgtg ggcgtctggg tgctgttgtt cctgggaatc ttgacgctct ttacctggcg
     2521 cctgaacgcc gcgttctgga aagaaattaa ctaacggacg ataacgcggt gcggagtcac
     2581 ggacgataac gcggtccggc tgcgccgcac acgcgaactt gtagagtggg ctgctgcgat
     2641 ggctcactct ttttgattta aggagccctc accatgatgg tcttgtattc gggaaccacc
     2701 tgccctttct cccaccgctg ccgcttcgtg ctgttcgaaa agggcatgga cttcgaaatc
     2761 cgcgatgtcg acctgtacaa caagccggaa gacatcaacg tgatgaatcc cta
//
LOCUS       DASWIY010000173        11819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2330300, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000173 DASWIY010000000
VERSION     DASWIY010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11819
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2330300"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..305)
                     /locus_tag="VGA59_06630"
     CDS             complement(60..305)
                     /locus_tag="VGA59_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719392.1"
                     /translation="MSPDEIRRLILAALADCERDGAERIRQQARSMRSGTDLWLLRGD
                     VYQLVACEHCEAEARRRVNDLLPAFEGWVPPRAMARL"
     gene            complement(381..1496)
                     /locus_tag="VGA59_06635"
     CDS             complement(381..1496)
                     /locus_tag="VGA59_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9719393.1"
                     /translation="MKHWHFLGAAALAWAGLTANAGAQIIVGQTVGVTGAVAATVKEA
                     GEGARLFIDSVNAKGGIKGEKIEVVTLDDKFNVKQTVENARVLIEEKNAVVLFMNRGT
                     PHTEAILPLLKKHGIALVGPSTGAMLMHNPVQPHVFNVRSTYQREAQKAVEHLHTLGI
                     SRIAVVHVDDSFGRDGLEGANKGFAKAQITAAAVVKADRAKPDYATIVPPIVASNAQS
                     VIWIGSGTAVADGIKALRAAGSAAQVVTLSNNASGGFIKLLGNASRGVIVTQVFPYER
                     SFNYPFVQEALSLAKAKGLSEVSPAMLEGFASAKVLVEALQRASPKPTREKVLAALEK
                     LHKFNLGGLEVSYSPTDHTGLDFADLSIIDGEGRFRR"
     gene            1942..8184
                     /locus_tag="VGA59_06640"
     CDS             1942..8184
                     /locus_tag="VGA59_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX9719394.1"
                     /translation="MTQGLIRIRGARQHNLKNLDLDLRTGQLTVVTGPSGSGKSSLVF
                     DTLYAEGQRRYVETFSAYARQFLERMDKPAVDRIEGVPPAIAIDQTNPVRSSRSTVGT
                     MTELNDHLKLLFARAGQLFDRKTALPVRHDTPETIYAELMARTGSGADGPPLGDDAPL
                     GGEARSASGGASTDPRLVITFPVELPAGTSAEELGQWLAASGFTRVQAEREVATPTGP
                     RKVLDVVADRFRLHATEKARAIEAIEVALKRGAGRMSVYVVEAVRSGREPEAMRQPAM
                     AVSHVLEATAAETWIADQVRNDSPKNPGHSGLEPESTGTAWIADQVRNDSKLGSDSNF
                     EVWKFSTGLHCPDSDLRYADPIPSMFSFNSAVGACETCRGFGRVIGVDYGLVIPNEKL
                     TLRAGAIKTIQTPAWAECQEDLMRHAETAGIPRDTPWYKLTPEQRHWVIHGTPSYKEG
                     NWNKQWYGIRRFFQYLETKSYKMHIRVLLSKYRSYTPCETCGGARLKTESLLWRIGSK
                     EDADAVLEPGKRFMPAGVQWTRGQLEALPGLCLHDLMLMPIDRLRRFFDRLQPSPQPS
                     PASGRGGSHAGEGRGEGNLEAPSGHSHPLPLGEGRGEGEQHALKLLYEEINTRLKYLV
                     DVGIGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMHRITE
                     AMKRLRDAGNTLVVVEHDPAVMLAADRIIDMGPGPGERGGQIVFDGATEDLRHADTLT
                     GAYLGARKHVGMGFKRAVTDATPRLILEGVREHNLKNLTLEFPLQRLVCVTGVSGSGK
                     SSLIQDVLAPALTRYFGKATETPGRHDRMLGADHLSDVVFVDQSPIGKTARSNPASYV
                     GAWDCIREIFAGAPLARQRGYTSSKFSFNSGDGRCPTCGGSGYEHVEMQFLSDVYLRC
                     PDCDGKRYRPEILEVMIERRSMEQAAGKSGSDSNYRAMNVADVLDLTVSEAASMFAND
                     REVIRALQPIVDVGLEYVKLGQPVPTLSGGESQRLKLAGFLAQAAKSATSSRQPTAVR
                     GTLFMFDEPTTGLHFDDIAKLMRALRKLLEGGNSLIVIEHNLDVIRAADWLIDLGPEG
                     GDAGGLLVAEGPPEEVKLHSTSHTGKALREYDSTLGLGSHIASEREKNWNLTPISLLP
                     QAGEGDKRGIKAIQIVNAREHNLKSLSVDIPHNKFSVVTGVSGSGKSTLAFDILFNEG
                     QRRYLESLNAYARSIVQPAGRPEVDAVYGIPPTVAIEQRLSRGGRKSTVGTTTEVWHF
                     LRLLYVKLGVQHCVNDGAEVRPQTPDSIAAQLMRRYRGQHIGLLAPLVINRKGVYTEL
                     AEWARPRGYTHLRVDGNFLPTHGFPRIDRFKEHTIELPVCDLDVAPANESLLREKLAL
                     ALEHGKGVLHVLAPLDGLRGAMLAGLGSHRHIGNVEVFSTRRACPVCSTSYPELDPRL
                     FSYNSKHGWCPECVGTGVKLTREQRKAFDDTVRSDDNKGREQTFAEPEVEDVVDEACP
                     ACHGARLNLQARNVKFDKTGIAEIAALSVVDVRRWIESLQVKGVLTAREEGIARDLIP
                     EIRSRLEFLEEVGLSYLTLDRGAPTLSGGEAQRIRLAAQLGSNLQGVCYVLDEPTIGL
                     HARDNQILLNALHKLGGKGNTLVVVEHDEDTIRRADHIIDIGPSAGKRGGRVVAQGSA
                     SDISAVPESVTGRYLLHAMKHPVKPRRALEREWSRLRLRGADLHNLRDIDVDIPLGRL
                     VAVTGVSGSGKSTLARDVLLHNVQAIVQQRYTKAGRDADAAGKHPPWVGCTGLNGYQV
                     IDRVLEVDQTPIGKTPRSCAATYIGFWDTIRRLFADTLEARARGYGPGRFSFNTGEGR
                     CPTCEGQGVRTIGMSFLPDVKVPCESCHGARFNPETLAVTWRGKSIGDVLRMEVDEAV
                     EFFAAMPNISHPLKLLEDVGLGYLTLGQPSPTLSGGEAQRIKLVTELSKVRDGILPPL
                     AFGEGPPVGGAALLGSGPAPRPTRRGNKAPHTLYVLDEPTVGLHMADVEKLVRVLHRL
                     VDGGHSVIVIEHDLDVIAEADWVIDLGPEGGNAGGKVVAEASPEQVVQRGTHTGNALS
                     AVLART"
     gene            8343..8840
                     /locus_tag="VGA59_06645"
     CDS             8343..8840
                     /locus_tag="VGA59_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719395.1"
                     /translation="MSIFLCKVLAPTVALGLLLSGCGGGSDDPEPTPIGASLTVSAAT
                     NATLNGVYSTNTVALAEVEKINPIGGDPEVCSFRFSGLAKPGGGLMDGDIRYIPGTNE
                     LRVIFVSISGLEFVSRDPTNAGVDRANNEIDLTGKLMTASTGVASTITLTGSMPMRGN
                     RPEGC"
     gene            complement(8950..9936)
                     /locus_tag="VGA59_06650"
     CDS             complement(8950..9936)
                     /locus_tag="VGA59_06650"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9719396.1"
                     /translation="MNPDRKLFLALAALALFALNLFAVPAHAQAPWPSQPIKIIVPFP
                     AGGASDQIGRLIGQKIGDELKVPVVIENRSGANGSVGATAFAKSPPDGYTLMVASIGV
                     FAINAGLFSKLPYDPLKDFAPISVLVSTPNVLVVPASSRFNSAQELARYMKQNPGKLS
                     YATSGTGSSDHLTGELFKKAVGGFAVHIPYRGGAPAMQDIIGGQADFMFSNLGPAMPF
                     IKGGKMKALMLTAARPAPQLPDVPTGAKVGIPLEVTSWQALAAPAGTPSAIIQRLHQI
                     AVKGMNSPEVKERMSNQAFDVVANSPAEFSDFLKKEVARWKVAVKESGAGLD"
     gene            complement(10058..11017)
                     /locus_tag="VGA59_06655"
     CDS             complement(10058..11017)
                     /locus_tag="VGA59_06655"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9719397.1"
                     /translation="MKTTAVTPADLRGSVIAVPPMPRMANGSIAPEANARLLDHLRAG
                     GVTTFMYGGNANFYNLGVAELGRVLDALTPLLAAGDWLIPSVGADFGKAAEQIALLRE
                     RAFPTALVLPLRFPAHPRGVARGLGKLASLYGKPLIAYVKDEGYIDNADLAALLREGS
                     VCAVKYAIVRHDPRQDPVLADLVHRMGPDHIVSGIGERPVIDHFRGFGLRAFTSGSTC
                     VAPALSNRIREALHTGDFATAMATRELFMPLEDARDAHSPIRVLHAAVQLAGIATAGP
                     LGEFLSVIEDKQLLDKLGAVALDLLARNEAALVRPRKQRSTIS"
     gene            complement(11104..>11819)
                     /locus_tag="VGA59_06660"
     CDS             complement(11104..>11819)
                     /locus_tag="VGA59_06660"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX9719398.1"
                     /translation="ITEISSPIERPRLSDTIYGQLLEEIMAGRFSAGDRLPTENQLAQ
                     RFSVSRPVVREALQRLQTDGVVIARQGSGTYVQRSPSQRVAELTSTVSLHEVLQVMEV
                     RMALEELSARLAARNRSEEQVRAIVQARQALGEVFGQQAAAIEADYAFHRAIAVASGN
                     PLLVEALDLLSERVKGGMNVTLSLTREASEQRRARVLDEHDRIVHAIRTGDAESAAIA
                     MRYHLDQARNRLLDRHLDR"
BASE COUNT         2003 a         3887 c         4012 g         1917 t
ORIGIN      
        1 gatcttcggc gcgggactct tcttgcaggt gctcgaatcc tgcgtggggg aagctcaggt
       61 cagaggcgcg ccatggccct ggggggcacc caaccttcga aggcgggcag caggtcgttg
      121 acgcgccgcc gtgcctcggc ttcgcaatgc tcgcaggcga ccagctggta gacgtcgccg
      181 cgaagcagcc agaggtccgt gcccgaacgc atgcttctgg cttgctgcct gatgcgctcg
      241 gcgccgtcgc gctcgcaatc cgccagcgcg gcgaggatca gccgccggat ctcgtcgggg
      301 ctcactgggg gccccgcggc gtcttgaggc gggcggccgg gaagccggat cttgaggaaa
      361 ctgccgaaca tggccgtcgt tcagcgccga aagcggccct cgccgtcgat gatcgacaga
      421 tcggcgaagt ccaggccggt atggtcggtg gggctatagc tcacctccag cccgcccagg
      481 ttgaacttgt gcaatttttc cagcgctgcc agcacttttt cgcgcgtggg cttgggcgac
      541 gcgcgctgca gcgcttccac cagcaccttg gcggacgcga aaccttcgag catggccggc
      601 gagacctcgc tcaggccctt ggccttggcc agcgacagcg cctcctgcac gaacggataa
      661 ttgaaggaac gctcgtaggg aaacacctgc gtgacgatca cgccgcggct ggcgttgccc
      721 agcagcttga tgaagccgcc cgaagcgttg ttggacaggg tcaccacctg cgccgcggac
      781 cccgccgcgc gcaaggcctt gatgccgtcc gccaccgcgg tgccggaacc gatccagatc
      841 accgactggg cgttcgacgc gacgatgggc ggcacgatgg tggcgtagtc gggcttcgcg
      901 cggtcggcct tgaccacggc ggccgccgtg atctgtgcct tggcgaatcc cttgttcgcg
      961 ccctccagtc catcgcggcc gaaggagtcg tccacgtgga cgacggcaat gcgcgagatt
     1021 cccagggtat gcaggtgctc gaccgccttt tgcgcttcgc gctggtaggt ggaccgcacg
     1081 ttgaaaacgt gaggctggac cgggttgtgc atcagcatgg cgccggtgga aggcccgacc
     1141 agcgcgatgc cgtgcttctt gagcagcgga aggatggcct cggtgtgggg tgtgccgcgg
     1201 ttcatgaaca ggacgaccgc gttcttttcc tcgatcagga cgcgtgcatt ctcgaccgtc
     1261 tgcttcacgt tgaacttgtc gtccagcgtg accacctcga tcttctcgcc cttgatgccg
     1321 cccttggcat tgacgctgtc gatgaaaagc cgcgccccct cgccggcttc cttgacggtc
     1381 gctgccacgg ctccggtgac gccgaccgtc tgtccgacga tgatctgcgc gccggcgttc
     1441 gccgtcagcc cggcccacgc gagcgccgcg gcgcccagaa agtgccaatg tttcacggtg
     1501 tctccttccg tttgcaaccg ggattccaac ggcgagatgt cgcccggcgc catggcggtg
     1561 cgcatctgct tcggcacgat caccgcgctc ccctgtcgac tcgaatgtgt tttctccgaa
     1621 tcgggcgcag tgtccctggt agcgatcgag cccaaacgat agggttggat ccacacggcg
     1681 ggcttgatgc tggtcaaacg ggctcaaggt cggggcttcg gaaactacgt agagatgaac
     1741 cccatggcgg ggcgcggtgc ctggcgtttc ctctggcgcc gctgtgcgcc gccacccgtc
     1801 agggttttat cagcgtttgt tgcgaaaaac gccggcagga cggatcaggc gcctggacct
     1861 gccatgggca aacccacatg acaggacgtg tcacccactc gtccctagaa tgttcggcca
     1921 actccagaaa tgaacccccg tatgacccag ggactgatcc gcatccgtgg cgccaggcag
     1981 cacaacctca agaacctcga cctcgacctg cgcaccggcc aactcacggt ggtgaccggg
     2041 cccagcggtt cgggcaagtc gagcctggtg ttcgacacct tgtacgcgga agggcagcgg
     2101 cgctacgtcg aaaccttctc ggcctacgca cgccagttcc tggagcgcat ggacaagccg
     2161 gcggtggacc gcatcgaggg cgtgccgccc gccatcgcga tcgaccagac caatccggtg
     2221 cgctcctcgc ggtccaccgt gggcacgatg acggagctca acgaccacct gaagctgctg
     2281 ttcgcgcgcg cgggccagct gttcgaccgc aagaccgcgc tgccggtgcg ccacgacacg
     2341 cctgagacga tctacgccga attgatggcg cgcaccggat ccggcgccga cgggccgccc
     2401 ctaggcgacg atgcccccct cgggggggag gcgcgaagcg cttcgggggg ggccagtacg
     2461 gatccgcggc tggtgatcac gtttccggtg gagcttccgg cgggaacctc ggctgaggaa
     2521 ctcgggcagt ggctggcggc cagcggcttc acgcgggtcc aggccgagcg cgaggttgcg
     2581 acgccgaccg gcccgcgcaa ggtgctggac gtggtggccg accgcttccg gctgcacgcc
     2641 acagagaagg cgcgcgccat cgaggcgatc gaagtggcgc tgaagcgggg tgcgggcagg
     2701 atgagcgtgt atgttgtcga ggcggttcgt tccggccgtg agccggaagc catgcggcaa
     2761 ccggcgatgg ccgtgtcgca cgtcttggaa gcgacggcgg cggagacatg gattgcggat
     2821 caagtccgca atgacagccc taaaaaccct ggtcattccg gccttgagcc ggaatccacg
     2881 gggactgcct ggattgcgga tcaagtccgc aatgacagca aattggggtc agattccaat
     2941 ttcgaggtct ggaagttctc gaccggcctg cactgccccg acagcgacct gcgctacgcc
     3001 gatccgattc cctcgatgtt ctcgttcaac tccgcggtcg gggcctgcga gacctgccgc
     3061 ggcttcgggc gcgtgatcgg cgtcgactac ggcctggtca tccccaacga gaaactcacc
     3121 ctgcgggccg gcgcgatcaa gaccatccag acgccggcct gggcggagtg tcaggaggac
     3181 ctgatgcgcc acgccgagac ggcgggcatt ccgcgcgata cgccctggta caagctcacg
     3241 ccggaacagc ggcactgggt gatccacggc acgcccagct acaaggaggg caactggaac
     3301 aagcagtggt acggcatccg ccgcttcttc caatacctgg agaccaagtc gtacaagatg
     3361 cacatccgcg tgctgctgtc caagtaccgc agctacacgc cgtgcgagac ctgcggtggt
     3421 gcgcgcctga agaccgagag cctgctctgg cggatcggaa gcaaggagga cgcggacgcc
     3481 gtgctggagc ccggcaagcg cttcatgccc gcgggcgtgc agtggacgcg cgggcaactg
     3541 gaggcgctgc cggggctgtg cctgcacgac ctgatgctga tgccgatcga tcggctgcgg
     3601 aggtttttcg atcggctgca accctcaccc caaccctctc ccgcaagcgg gagagggggt
     3661 tcgcacgcgg gagaaggcag gggtgagggc aaccttgaag ctccttcagg ccattctcac
     3721 cctctccctc tgggagaggg caggggtgag ggcgaacagc atgctctcaa gctcctctac
     3781 gaggaaatca acacccggct gaagtacctg gtggacgtcg gcatcggcta tctcacgctg
     3841 gaccggcaaa gccgcacctt gtcgggcggc gaggtgcagc ggatcaacct gacgacggcc
     3901 ctgggtactt cgctcgtgaa cacgctgttc gtgctcgacg aacccagcat cggcctgcat
     3961 ccgcgcgaca tgcaccgcat caccgaggcg atgaagcggc tgcgtgacgc cggcaacacg
     4021 ctggtcgtgg tggagcacga cccggcggtg atgctggccg ccgaccgcat catcgacatg
     4081 ggcccgggtc cgggcgagcg cgggggacag atcgtgttcg acggcgccac cgaggacctg
     4141 cgccatgccg acacgctcac cggcgcctac ctgggagcgc gcaagcacgt aggcatgggt
     4201 ttcaagcgcg cggtgaccga cgcgacgccg cgcctgatcc tggaaggcgt gcgcgaacac
     4261 aacctgaaga acctgacgct ggagtttccg ctgcagcggc tggtctgcgt caccggcgtg
     4321 tccggctccg gcaaatcgag cctgatccag gacgtgctgg cgccggcgct cacccgctac
     4381 ttcggcaagg cgacggaaac gccgggccgg cacgaccgca tgctgggcgc cgaccacctg
     4441 agcgacgtcg tgttcgtcga ccagtcgccg atcggcaaga cggcgcgatc caatcccgca
     4501 agctatgtcg gcgcgtggga ctgcattcgc gagatcttcg cgggtgcgcc gcttgcccgg
     4561 cagcgcggct acacgtcttc caagttcagc ttcaacagcg gcgacggccg ctgccccacc
     4621 tgcggcggct ccggctacga gcatgtggag atgcagttcc tgtcggacgt gtacctgcgc
     4681 tgccccgact gcgacggcaa gcgctatcgg cccgagatcc tggaagtgat gatcgaaaga
     4741 aggagcatgg aacaggcggc gggaaaatcg gggtcagatt ccaattaccg cgcgatgaac
     4801 gtcgccgacg tgctggacct cacggtgagc gaggcggcgt cgatgtttgc caacgaccgc
     4861 gaggtgatcc gcgcgctgca gccgatcgtg gacgtcgggc tcgagtacgt gaagctgggc
     4921 cagccggtcc cgaccttgtc ggggggtgaa tcgcagcgcc tgaagctcgc gggcttcctg
     4981 gcgcaggcgg ccaagagcgc cacctcgagc cggcagccga ccgccgtccg cggcacgctg
     5041 ttcatgttcg acgagccgac gacgggcctg cacttcgacg acatcgccaa gctcatgcgc
     5101 gccttgcgca aattgctgga aggcggcaat tcgctgatcg tgatcgagca caacctggac
     5161 gtgatccgcg ccgccgactg gctgatcgac ctgggccccg aaggcggcga cgccggcggg
     5221 ctgctggtcg ccgaagggcc gcccgaagaa gtgaagctgc attccacttc gcacacgggc
     5281 aaggccctgc gcgaatacga cagcacgctg gggctcggtt cgcatatcgc atccgagcgc
     5341 gaaaagaatt ggaatctgac cccaattagt cttctcccgc aagcgggaga gggagacaag
     5401 agaggaatca aggccatcca gatcgtcaac gcccgcgagc acaacctcaa gtcgctgtcc
     5461 gtagacatcc cgcacaacaa attcagcgtg gtcaccggcg tgtcgggatc gggcaagtcc
     5521 acgctcgcct tcgacatcct gttcaacgag ggacagcggc gctacctcga atcgctcaac
     5581 gcctacgcgc gcagcatcgt gcagccggcg ggccggcccg aagtggacgc ggtctacggc
     5641 attccgccca cggtggcgat cgaacagcgg ctgtcgcgcg gcggccgcaa gtcgaccgtg
     5701 ggcacgacca ccgaggtctg gcatttcctg cgcctgctgt acgtcaagct cggcgtgcag
     5761 cactgcgtca acgacggcgc cgaagtgcgg ccgcagaccc cggacagcat cgccgcccag
     5821 ctgatgcggc gctaccgcgg ccagcacatc ggcttgctgg cgccgctggt gatcaaccgc
     5881 aagggtgtgt acacggaact cgcggaatgg gcccggccgc gcggctacac gcacctgcgt
     5941 gtcgacggca attttctgcc gacccacggc tttccgcgca tcgaccgttt caaggaacac
     6001 accatcgagc tgccggtgtg cgacctcgac gtggcgcccg ccaacgagag cctgctgcgc
     6061 gagaagcttg cgctcgcgct ggagcacggc aaaggggtgt tgcatgtgct cgcgccgctg
     6121 gacggcctgc ggggcgcgat gctggccgga ctcggctcgc accgccacat cggcaacgtc
     6181 gaggtgttct ccacccggcg tgcctgcccg gtgtgcagca ccagttaccc cgagctggac
     6241 ccgcgcctgt tttcctacaa cagcaagcac ggctggtgcc cggagtgcgt gggcacgggc
     6301 gtgaagctca cccgcgagca gcgcaaggcc ttcgacgata cggtgcggtc cgacgacaac
     6361 aagggccgcg agcagacctt cgccgagccg gaggtggaag acgtggtgga cgaagcctgt
     6421 ccggcctgcc acggcgcccg gctgaacctg caggcgcgca acgtgaagtt cgacaagacc
     6481 ggcatcgccg agatcgcggc cctgtcggtc gtcgatgtac gcaggtggat cgaaagcctg
     6541 caggtcaagg gggtgttgac ggcgcgcgag gaaggcatcg cacgcgacct gattcccgag
     6601 atcaggagcc gcctcgagtt tctggaagag gtcggcctga gctacctcac gctggaccgc
     6661 ggcgcgccga ccctgtccgg tggcgaagcc cagcgcatcc ggctcgccgc ccagctgggc
     6721 tcgaacctgc aaggcgtgtg ctatgtgctg gacgagccga ccatcggcct gcacgcgcgc
     6781 gacaaccaga tcctgctgaa cgcgctgcac aagctgggcg gcaagggcaa cacgctggtg
     6841 gtggtggagc acgacgagga cacgatccgc cgcgccgacc acatcatcga catcgggccc
     6901 agcgccggca agcgcggcgg ccgggtggtg gcgcagggat cggcttcgga catttcggcc
     6961 gtgcctgaat cggtcaccgg ccgctacctg ctgcatgcga tgaagcatcc ggtgaagccg
     7021 cggcgggcgc tggagcgcga gtggtccagg ctgcggctgc gcggggccga cctccacaac
     7081 ctgcgtgaca tcgacgtcga cattcccctg ggccgactgg tcgcggtgac cggcgtttcc
     7141 ggctccggca agtccacgct ggcacgcgac gtcctgctgc acaacgtgca ggcgatcgtg
     7201 cagcagcgct acaccaaggc cggccgcgat gccgatgccg cgggcaagca cccgccctgg
     7261 gtcggctgca ccggcctgaa cggttatcag gtgatcgacc gcgtgctgga ggtggaccag
     7321 accccgatcg gcaagacgcc gcgttcctgc gccgccacct acatcggttt ctgggacacc
     7381 atccgcagac tgttcgccga tacgctggag gcgcgcgccc gcggctatgg tcccggccgc
     7441 ttctccttca acaccggcga agggcgctgc cccacctgcg aaggccaggg cgtgcgcacg
     7501 atcggcatga gtttcctgcc ggacgtgaaa gtgccttgcg aatcctgcca tggcgcccgc
     7561 ttcaatcccg agacgctggc cgtgacctgg cgcggcaaga gcatcggcga cgtgctgcgg
     7621 atggaagtgg acgaagccgt ggagttcttc gccgccatgc ccaacatcag ccatccgctc
     7681 aagctgctgg aggacgtcgg cctgggttac ctgacgctgg gccagccatc gcctaccctg
     7741 tccggcggcg aggcccagcg catcaagctc gtcaccgagt tgtccaaggt gcgcgacggg
     7801 attttgcccc ccctcgcctt cggcgaaggc ccccccgtgg ggggcgcagc cttgcttggg
     7861 agcggcccgg cgccgcggcc cacgcgccgc ggcaacaagg cgccgcacac gctgtacgtg
     7921 ctggacgagc cgaccgtggg gctgcacatg gccgacgtcg agaagctggt ccgcgtgctg
     7981 caccggctgg tcgatggcgg ccacagcgtg atcgtgatcg agcacgacct ggacgtgatc
     8041 gccgaagccg actgggtgat cgacctgggc cccgaaggcg gcaacgcggg cgggaaggtg
     8101 gtggcggagg cttcgccgga acaggtggtg cagcggggca cccacacggg caacgcgctg
     8161 agcgcagtgc tggcgcggac gtgaaccgat gagcttaggg cctttgagca ggccctaccc
     8221 gctcgacgcg cggcgattcc tacgttgcca actttcctac gttgccaact gatacgtgcg
     8281 gacaaactat ggcgacacag cggctgtagc ctgcagtggg tccattcaca cgaggtaacg
     8341 ccatgagcat ttttttgtgc aaggtcctcg ctcccaccgt cgcgctgggg ctgctgttgt
     8401 cgggctgcgg tggaggtagt gacgatcccg agcccacgcc catcggcgcc tcgctgaccg
     8461 tgtcggcagc caccaacgcg accctcaacg gggtgtattc caccaatacg gtggccctgg
     8521 cggaggtgga gaagatcaat ccgatcggcg gagaccctga agtgtgcagc ttccgcttct
     8581 ccggcctcgc caaaccgggt ggcggattga tggacggcga catccgctat atcccgggaa
     8641 ccaacgagct gcgcgtcatc ttcgtttcca tcagcggcct ggagttcgtt tcgcgggatc
     8701 cgacgaatgc cggggtggac cgcgccaaca acgagatcga cctgaccggc aagctcatga
     8761 ccgcgtccac gggtgtcgca tcgacgatca cgctgaccgg cagcatgccc atgagaggga
     8821 accggcctga aggctgctga cgcatcgcta gacgtcttct tgtagggtgg gcaatttcgc
     8881 ccaccgcgca gcttcgaaaa gatcggtggg cagcaagtgc ccaccctacc gggattcccg
     8941 caggtcccct caatccagac cggcgccgga ttccttcacg gcgactttcc agcgcgccac
     9001 ttccttcttg aggaagtcgc tgaactccgc cggcgagttg gcgaccacgt cgaaagcctg
     9061 gttgctcatg cgctccttca cttcgggcga gttcatgccc ttcaccgcga tctggtgcag
     9121 ccgctggatg atcgcgctcg gcgtgcccgc gggcgcggcg agcgcctgcc agctcgtgac
     9181 ctccagcggg atgccgacct tggcgccggt gggcacgtcg ggcagctgcg gtgcgggtcg
     9241 ggcggccgtc agcatcagcg ccttcatctt gccgcccttg atgaacggca tggcgggccc
     9301 gaggttgctg aacatgaaat cggcctggcc gccgatgatg tcctgcatcg ccggcgcgcc
     9361 gccgcggtag ggaatgtgca ccgcgaaacc gcccacggct ttcttgaaca gctcgcctgt
     9421 caggtggtcg gacgagccgg tgcccgaggt cgcataggac agcttgcccg ggttctgctt
     9481 catgtacctc gccagttcct gcgccgagtt gaaacgcgag ctggccggca ccaccagcac
     9541 gttgggcgtg gacaccagca ccgagatcgg cgcgaagtcc ttgagcgggt cgtacggcag
     9601 cttgctgaac aggccggcat tgatcgcgaa cacgccgatg ctcgcgacca tcagcgtgta
     9661 gccgtcgggc ggcgacttgg caaacgccgt cgcgcccacc gagccgttgg cgccggagcg
     9721 gttctcgatc acgaccggga ccttcagctc gtcgccgatc ttctgcccga tcagccggcc
     9781 gatctggtcg gacgcgccgc cggcggggaa gggcacgatg atcttgatcg gctggctggg
     9841 ccatggggcc tgcgcatggg ccggcaccgc gaacaggttc agcgcgaaca gcgccagtgc
     9901 ggccagggcg aggaagagct tgcggtcggg attcatgctt gtctccttat tgggggatcg
     9961 gttgagcgat ggcttggtca tcggcgtgag catgcggccg cttgttccgg catcgggcgc
    10021 gccgcctgca ggatgcctac ggggttcgag gtggcgctca tgagatcgtg ctgcgctgtt
    10081 tccgcggccg caccagcgcg gcttcgttgc gcgccagcag gtccagcgcg acggccccga
    10141 gcttgtccag cagctgtttg tcttcgatca cggacaggaa ttcgccgagc ggccccgccg
    10201 tcgcgatgcc cgcgagctgg accgcggcgt gcagcacgcg aatcggcgaa tgggcatctc
    10261 gtgcatcctc cagcggcatg aacaactcgc gcgtggccat ggcggtggcg aagtctcccg
    10321 tgtgcagggc ttcacggatg cggttggaca gggcgggcgc gacgcaggtc gagccggagg
    10381 tgaaggcccg caggccgaag ccgcgaaaat gatcgatcac cggccgctcg ccgatgccgc
    10441 tgacgatgtg atcgggcccc atgcgatgca ccaggtcggc cagcaccggg tcctgcctgg
    10501 ggtcatggcg cacgatcgcg tacttgacgg cgcacaccga gccttcccgc agcagcgccg
    10561 cgaggtccgc gttatcgatg tagccctcgt ccttgacgta ggcgatcagc ggcttgccat
    10621 agagggaggc cagcttgccc aggcccctgg ccacaccccg cggatgggcc ggaaagcgca
    10681 gcggcagcac cagcgcggtc gggaaggcgc gctcgcgcaa cagcgcgatc tgctcggccg
    10741 ccttgccgaa gtcggcgcct acggacggga tcagccagtc gccggctgcc agcagcggcg
    10801 tcagcgcgtc cagcacccgg ccgagctcgg cgacgcccag gttgtagaag ttggcattgc
    10861 cgccatacat gaacgtggtg accccgccgg cgcgcaggtg atcgagcagg cgggcgttgg
    10921 cctcgggggc gatgctgccg ttggccattc ggggcatggg cggcacggcg atgacggagc
    10981 cgcgcaggtc ggccggtgtg acggcagtgg tcttcaaagc gaatccttgg gacagaaatc
    11041 agggcaaaca ggccacgcgc ctatttgttt gacaacgtta ccaattattc gccgtgttgt
    11101 aaatcaacgg tccagatgcc ggtcgagcaa gcgattgcgg gcctggtcga ggtgatagcg
    11161 catcgcgatg gccgcactct cggcgtcacc ggtacggatg gcgtgcacga tgcggtcgtg
    11221 ctcgtcgagc acgcgggcgc gccgctgttc cgacgcttcg cgcgtcaacg acagggtgac
    11281 gttcatgccg cctttgacgc gctccgaaag caggtccagc gcttcgacca gcaaggggtt
    11341 gcccgatgcg accgcgatcg cgcggtggaa cgcgtagtcg gcttctatgg ccgcggcctg
    11401 ttgcccgaac acttcgccca gggcctgccg ggcctgcacg atggcgcgca cctgctcttc
    11461 gctgcgattg cgcgcggcca gcctggccga gagttcttcc agcgccatgc gcacttccat
    11521 cacctgcagc acttcgtgca ggctgacagt gctggtgagt tcggccacgc gctgcgaagg
    11581 gctgcgctgc acataggtgc ccgagccctg gcgcgcgatg acgacgccgt cggtctgcag
    11641 gcgctgcaac gcctcccgca ccaccgggcg cgataccgag aagcgttgcg cgagctggtt
    11701 ctcagtgggc agccggtccc cggccgagaa gcgcccggcc atgatctcct cgagcagctg
    11761 gccgtagatc gtgtcggaca gccggggtcg ctcgatgggg gaggaaatct cggtgatgg
//
LOCUS       DASWIY010000174         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1201403, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000174 DASWIY010000000
VERSION     DASWIY010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201403"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1441
                     /locus_tag="VGA59_06665"
     CDS             join(303..570,570..1441)
                     /locus_tag="VGA59_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX9719399.1"
                     /translation="MVKKSARRNRRTHTAVFKARVALAALREDRTMAELCKEFELHPN
                     QITEWKRQLLERAAEVFDTSARPDPVNLAPLHAKIGQLALENDFLGRRAHQSGIAERK
                     AMIDRDHQLSITRQAKLLEISRGTVYYLPRPVSPGDLALMRKIDELHLEHPFMGARML
                     RDQLARQGIRAGRRHIGTLMLRMGIEALAPQPGTSKAAPGHKIYPYLLRKLAIIRANQ
                     VWALDTTYIPMARGFVYLTAVVDVASRRVLAHKVAITLEAIHAKEVIEQAFARYGTPE
                     IVNTDQGSQFTAEEFTSVVLAKGCRLSMDGRGAWRDNVFVERLWRSVKYERVYLKAYD
                     GVSAARSDIAEYLDWYNTHRGHSSLERLTPNEKYLAALPPMALAA"
BASE COUNT          356 a          518 c          531 g          290 t
ORIGIN      
        1 tcgccgccgt aggtctcctt gacgacgttg cacatctcgc cgaccgcgag ctcgacgtcc
       61 tcgaacttga cgccgggctt gagcttcacg gtgttgaaca gcatcttggc gccgaacggc
      121 agggtgatcg gttcgaacat cagcttgctc cgtaagtaag agaatctttc gacaacccca
      181 ttttgcccag gtttcaggag acaggaccat gagaagtggc tccgcaaatc tgaacagcgc
      241 tataagtgga aactccgggc attatgctgg gccgaagacg gtctggcgca aaggagtgat
      301 tgatggtcaa gaaatcagcg cggcgcaatc gccgcacgca cacggcggtg ttcaaggcac
      361 gggtagctct ggcggccctg cgagaagatc gcacgatggc cgagctttgc aaggaattcg
      421 aattgcatcc caaccagatc accgaatgga agcggcaact gcttgagcgc gcggccgagg
      481 tctttgacac cagcgcgcga cccgatccgg tgaacctggc accgctgcac gccaagatcg
      541 gccaactggc gctggagaac gattttttgg aaggcgcgct caccaaagcg ggattgctga
      601 gcgcaaagcc atgatcgacc gtgaccacca actgagcatc acgcgccagg ccaagctgct
      661 ggagatcagc cggggaacgg tgtactacct gccccggcct gtcagcccgg gcgatctggc
      721 gctcatgcgc aagatcgacg agctgcatct ggagcacccc ttcatggggg cgcgcatgct
      781 gcgcgaccaa ttggcccggc aaggcatccg cgccggacgg cgtcacatcg gaacgctgat
      841 gctgcgcatg ggcatcgaag cgctggcgcc gcagccgggc accagcaaag ccgcgccggg
      901 acacaagatc tacccctatc tgttgcgtaa gctcgcgatc atccgcgcca atcaggtctg
      961 ggcgctggac acgacctaca ttcccatggc gcgcggtttc gtctacctca ccgccgtggt
     1021 ggacgtggcc tcgcgccgcg tgctggcgca caaggtggcg atcaccctgg aagcgattca
     1081 cgccaaggag gtgatcgagc aggccttcgc ccggtacggc acgcccgaga tcgtcaatac
     1141 ggaccaggga agccagttca cggccgagga attcaccagc gtcgtgctgg ccaagggctg
     1201 caggctgtcc atggacgggc gcggcgcctg gcgcgacaac gtgttcgtcg agcgcctgtg
     1261 gcgcagcgtg aagtacgaac gggtgtacct caaggcctac gacggcgtga gtgcggcgcg
     1321 cagcgacatt gccgagtatc tggactggta caacacgcat cgcggtcatt ccagtctgga
     1381 gcgacttacg ccgaacgaaa agtacctggc tgcgctgccg cccatggcgc tggccgcgta
     1441 agatgaatct cgtggtgtgc cccgagttgc ccaccgcgtc ggtcgttcgt cgcaagcgac
     1501 gcccgccgcc gtggacaact ctgcacctcc aaacccgccc ggagtccact tataaatcgg
     1561 ggaaagttgt tcaaacaacc ggagccactt cttgggatcg ttagtaagaa aacggcgagt
     1621 acaagacgtt gccaagtatg aagccattga ggcagcgctg ctggagcgct tggggacgct
     1681 aagactgctg cgact
//
LOCUS       DASWIY010000175         7484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_837639, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000175 DASWIY010000000
VERSION     DASWIY010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837639"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VGA59_06670"
     CDS             <1..707
                     /locus_tag="VGA59_06670"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9719400.1"
                     /translation="VVVQATNGRVPVVAGVAATTIADAVEQVRSYEALGVDGILAILE
                     AYFPIAEDGVVAYFSAIANATALPVVLYTNPQFQRSDLTLPAIERLSRIDNIRYLKDA
                     SSDTGRLLGIMNAVGDRIRVFSASAHIPACVMLIGGVGWMAGPACVVPRQSVRLYDLC
                     RAGQWQEAMALQRRLWRMNGAFAKYNLAACIKGGLELQGYEVGAPLPPQTPLSEAARA
                     EIKRVLMDLGALAPAD"
     gene            737..1540
                     /locus_tag="VGA59_06675"
     CDS             737..1540
                     /locus_tag="VGA59_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9719401.1"
                     /translation="MEQKQVSDTWDRGSAYEQYVGRWSRRVAPLLLSWLDIPAGRRWL
                     DVGCGTGALCAAITDHCAPSAVIGVEPSPGYLKTALENLAGRAVLHQGSATAIPLDDA
                     AVDVVVSGLVLNFVPDPDAALHEMARVTAKGGTIAGYVWDYAGKMELMRYFWDAAVEL
                     DPEARKLDEGIRFPLCNPEALEALFSGAGLKQVVVKPIDIPTPFANFDDYWQPFLGGQ
                     GPAPGYAISLEEPARARLREHIRQRIPGAVDGSISLIARAWAIRATVPD"
     gene            1537..1791
                     /locus_tag="VGA59_06680"
     CDS             1537..1791
                     /locus_tag="VGA59_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719402.1"
                     /translation="MSEPGPMRTHRWWLALLAALLTPWIGPHIDRYVPLGWVLLQARD
                     NSPDAGFWVLAGVLLGVCYLVWVAVLSGVAAISSRRRREP"
     gene            1867..3495
                     /locus_tag="VGA59_06685"
     CDS             1867..3495
                     /locus_tag="VGA59_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX9719403.1"
                     /translation="MLEAKLKRARECYRRNAWSEAHQLLSLADESAPLGAEDLELLAT
                     SAYLIGRDLDFHQSLERAHHVWLRQGEQCRAARCAFWMGLTLLLQGDAGRGNGWLARA
                     KRLVEDRECVENGYLLLPIAEQCLGDRDAEAALAAATDASDVGGRYGDADLTACARHL
                     QGRALIQQGRVQRGVQLLDEAMVAVSGGEVSPIMTGLIYCSVIAACHRVFALGRAREW
                     TFALSRWCEQQPQLVAFTGTCLVDRAEVMQFRGAWADAMSEACLARERVSHAGESKAP
                     AAAFYRQGEIHRLRGEFAAAEEAYRTASRMGAEPQPGLALLRMAQGNTDAACAAIRRV
                     LSATTEPLERAKLLPAYVEVAQAAGHMDEARAAATELDDTARRYPTDVLRAMAAHAQS
                     AVELAEGRAEAALAALHHAFELWQRGEAPYETACVRLLTALACRELGDVESADLEFEA
                     ARGLFERLGAIPDIARLDSLRNRTTHAGQHGLTSRELQVLRQIAAGKTNKAIAGELCV
                     SERTIDRHVSNLLTKLGVPSRAAATAYAYNHKLI"
     gene            3659..4537
                     /locus_tag="VGA59_06690"
     CDS             3659..4537
                     /locus_tag="VGA59_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9719404.1"
                     /translation="MSIQASTMDSKVTQASHEPASDTRDAWDRIAPGYDRTNTPTQMA
                     IAGKGLGIAGLASGMRFLDVAAGSGALAIPAARLGARVTAIDLSPGMLELLAARARKD
                     GLVVETRVMDGHALQFADDTFDVAGSQFGVMLFPDMPKAVRELVRVVKPGGRVLINAY
                     GDPEEIEFLGFLVTAVQSVRPGFDGPPADPPPLEFQLADPGRLRQELSAAGLKDVKVE
                     AITEITEHATGNDLWEWLVWSNPIVECVLGGMLNLTEDERGVVRQNLDKLVHARAAGS
                     RAAKLTNPVNIGVGTK"
     gene            4782..5189
                     /locus_tag="VGA59_06695"
     CDS             4782..5189
                     /locus_tag="VGA59_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEX9719405.1"
                     /translation="MMDVALPYRVLADAVLVAHVGIVLFVVAGFVLVVAGNVRQWRWV
                     NHLWFRLAHLAAIAVVVAESWIGITCPLTTLEMWLRARAGVSTYSEGFVEYWLQRVLY
                     YDAPPWVFTLGYTLFGLLVAATWWYFPPARRSR"
     gene            complement(5270..5845)
                     /locus_tag="VGA59_06700"
     CDS             complement(5270..5845)
                     /locus_tag="VGA59_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX9719406.1"
                     /translation="MTITITPADWLSFLTHFLALSLLSVGGAITTAPDMHRFLVDRQG
                     WLTDPQFSASIAIAQAAPGPNVLFVALLGWNVGLNAGGLPTALLGMAVAMVAIMVPST
                     TLTYVTARWSHANRELRSVRAFKLGMTPIVIALLIATGWILATGSRYDAARDWRLWLV
                     AIVSTLLVWRTRIHLLWLLGAGAVLGWMGWV"
     gene            complement(5901..6506)
                     /locus_tag="VGA59_06705"
     CDS             complement(5901..6506)
                     /locus_tag="VGA59_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX9719407.1"
                     /translation="MSSSPEAAATTSIEQRPQPHSLTDLFLSFTFLALQGFGGVLAVV
                     QRELVEKKRWLTRDEFVEEWAVAQIMPGPNVVNLALMLGDRYFGLRGAMAALAGMLTF
                     PLLVVLCLALVYAQYADNLNVAGALRGMGAVAAGLITATGMKLLGAIRKNPLGVPLCI
                     VFGVASFAGIALLRWPLAWVLLGLGGLSCVLTYRKMGRTGA"
     gene            complement(6532..>7484)
                     /locus_tag="VGA59_06710"
     CDS             complement(6532..>7484)
                     /locus_tag="VGA59_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9719408.1"
                     /translation="RAEHGQRSVGAEAINLALLAAAAAGVLVGAGIVASRFAVGEVPP
                     LTLAMLRYAIGFCCLLPFAWRAWSSAQQARRTSAAVNRKVDLLAMAALGIGQFGVLIA
                     LLNYGLQHVAAAQAALIFSLFPLLTLLMSAALGRERVTASLMLGVLISIAGVALALSP
                     KLGQAQSGHWWGELAVAASAFIGALCSVLYRPYLERYPTVPVSAFAMAASVLFLALLA
                     LGEHWPARVVTFSLQAWAAVSFIGVSSAIGYFCWLYALKHESPTRVTVFLALNPVTAA
                     LLGWALLGEALHPAASGALALIGGGLWLATRPAITRVPAA"
BASE COUNT         1329 a         2401 c         2537 g         1217 t
ORIGIN      
        1 aggtcgtggt acaggcaacg aacggccggg tgccggtggt ggcgggtgtt gccgccacga
       61 cgattgccga cgccgtcgaa caggtcaggt cctacgaggc gctgggggtc gacggcatcc
      121 tcgcgattct cgaagcctac tttccgatcg ccgaggatgg agtcgtcgcc tatttctccg
      181 cgatcgcgaa tgcaacggcg ttgcctgtgg tgctgtacac caacccgcag tttcagcgca
      241 gcgacctcac gctgccggcc atcgagcgcc tgagcaggat agacaacata cgctacctca
      301 aggacgcctc gtccgatacc ggccggcttc tcggcatcat gaatgccgtc ggagaccgca
      361 tcagggtgtt cagcgcttcg gcacacatcc cggcatgtgt gatgctgatc ggaggggtgg
      421 ggtggatggc cggtcccgcc tgcgtggtgc ctcggcaaag cgtgcggctg tacgacttgt
      481 gccgggcggg ccagtggcag gaggccatgg cgttgcagcg gcggctctgg cgcatgaatg
      541 gcgcgttcgc caaatacaac ctggccgctt gcatcaaggg cggcctggaa ctgcagggct
      601 atgaagtcgg tgcaccgctg ccaccgcaaa cgccgctgtc cgaagcagcc cgggcggaaa
      661 tcaaacgcgt tctcatggac ctgggtgcgc tggcgccggc ggactgagaa agcacgcatt
      721 gaaaggagtg tgaccgatgg aacagaaaca ggtgtccgat acatgggacc gcggaagcgc
      781 gtatgagcag tacgttggcc gctggagccg ccgcgttgcg cctttgttac tctcatggct
      841 ggatattccg gccggccggc gttggctcga cgtgggatgc ggaacaggag cgctgtgcgc
      901 ggcgatcacg gaccactgcg ccccgtctgc cgtcatcggt gtcgagcctt cacccggata
      961 tctcaagacc gcattggaga atctcgcggg ccgggccgtg cttcaccaag gcagcgcgac
     1021 ggcgatcccg ctggacgatg ccgcagtcga tgtggtggtc tcgggactgg tcctgaactt
     1081 cgttcccgat ccggatgccg cgcttcatga gatggcgcgg gtgacggcca agggcggcac
     1141 gatcgcaggc tacgtctggg attacgccgg caagatggaa ctgatgcggt acttctggga
     1201 tgcagcagtc gagctcgacc cggaggcccg aaaactggac gagggcatcc ggttcccgct
     1261 gtgcaatccg gaggcgctcg aggcgctctt cagcggcgcg ggactgaagc aagtcgtggt
     1321 caagcccatc gatatcccga cgccattcgc gaatttcgac gactactggc agccgttcct
     1381 gggcggccag ggcccggctc ccgggtacgc aatctcgctc gaggagccgg cgagagcgcg
     1441 gctgcgcgag cacatacgac agcgcatccc cggcgccgta gacggttcga tctccctgat
     1501 tgcgcgggcg tgggcaattc gcgccaccgt ccccgattga gcgagccggg tcccatgcgc
     1561 acgcatcgct ggtggctggc acttcttgcg gccttgctca ctccctggat cgggccgcat
     1621 atcgatcgct acgtgcccct cgggtgggtg ctgcttcaag cccgtgacaa ttcgcccgat
     1681 gccgggttct gggttcttgc cggcgtcttg ctcggagtct gctacctggt gtgggtcgcg
     1741 gtcctgagcg gggtcgccgc catctcctca cggcgtcgcc gcgagccatg aggtcccgcg
     1801 gccagcgcat gagctgaggc tgaagacaaa tgctcacgga gcgggcggca cgcaataatc
     1861 caggacatgc tcgaagcgaa actcaaacgg gctcgcgagt gctatcggcg caatgcatgg
     1921 agtgaagccc atcagctgct ctcgctcgcg gatgaatcag ccccccttgg cgcggaggac
     1981 ctcgagctgc tggccacatc agcctacctg atcggccggg acctcgattt tcaccaatcc
     2041 ctggaacgtg cccatcacgt ctggctgcgg caaggcgagc agtgccgcgc ggctcgctgt
     2101 gctttctgga tgggtctgac gttgctgctc cagggcgacg cagggcgcgg gaatggttgg
     2161 ctggctcgcg cgaagcgcct cgtggaggac cgcgaatgcg tcgagaacgg ttatctgctg
     2221 ctgccgatcg cggagcagtg tctcggcgac agggacgctg aagcggcgct cgcagcggca
     2281 accgatgcat ccgatgtcgg tggtcgctat ggagatgcgg acctgaccgc ctgcgcccgt
     2341 cacctgcagg gccgtgcgct catccagcaa gggcgggtcc aacgcggcgt gcaactgctg
     2401 gacgaggcga tggtcgcggt gagcggcggc gaggtctcgc cgatcatgac gggcttgatc
     2461 tattgcagcg tgatcgcggc ctgtcaccgg gtcttcgcgc tgggccgggc gcgcgagtgg
     2521 acgtttgcat tgagccgctg gtgtgagcag caacctcaac tggtggcctt cacggggacc
     2581 tgccttgtcg atcgcgcgga agtcatgcag tttcgtggcg cctgggccga tgccatgagc
     2641 gaggcgtgcc tcgcgcgcga gcgcgtgtcc cacgccggcg agtcgaaggc gcctgccgcg
     2701 gcattctacc gccagggcga gatccatcgc ttgcgcggag agttcgcggc ggccgaggag
     2761 gcctatcgga ctgcgagccg catgggcgcg gagccccaac cggggctcgc gctgctgcgc
     2821 atggcccagg gaaacaccga cgccgcctgc gcggcgatcc gccgggtcct gagcgcgacc
     2881 accgaaccgc tggaacgtgc gaagctgctt cccgcctacg tcgaagtcgc acaggccgcg
     2941 ggccacatgg atgaagctcg cgctgctgca accgagttgg atgacacagc gcggcgctac
     3001 cccaccgacg tactgcgggc catggccgcg cacgcgcaaa gcgcggtcga actggccgaa
     3061 ggccgcgcgg aggctgcgct ggcggcgcta caccacgcgt tcgaactttg gcaacggggc
     3121 gaggcaccgt acgagactgc ttgcgtgcgt ctgctgaccg cgctcgcgtg tcgcgaactc
     3181 ggcgacgtcg aatccgcgga tctcgagttc gaggcggcca ggggcctgtt cgaacgcctg
     3241 ggggccattc cggatattgc tcgcctcgac tcgcttcgaa accgcacaac gcacgcgggc
     3301 cagcatgggc tgacgtcgcg cgagcttcag gtcctgcgcc aaatcgccgc gggcaagacg
     3361 aacaaggcga tcgcgggcga gttgtgcgtg agcgagcgca cgatcgacag gcacgtgagc
     3421 aacctgctca ccaagcttgg cgtgccttcc agggccgccg ccacggccta cgcgtacaac
     3481 cacaagctga tctgatccat gcccttgtct gtcattgcgg gcctcaacag cttgggcgct
     3541 ggcggaaatt acccagcctg gccggcatcg cgtttgggta atctcgccga agcgcggtcc
     3601 ctgaatgtct ggcaactttg gcatgcggac ggttccgcaa tctgccaagg aggacacgat
     3661 gagcattcaa gcttctacga tggacagcaa ggtgacccag gcatcgcacg agccggcttc
     3721 agatacgcgt gacgcgtggg accggatcgc gccgggctac gaccggacca acacgccgac
     3781 acagatggcg atcgccggaa aggggctggg tatcgccggc ctggcgtccg gtatgagatt
     3841 cctcgacgtg gccgccggaa gcggtgccct cgccatcccg gccgcgcggc tcggggcacg
     3901 ggtcacggcg atcgatctgt ctcccggcat gctcgagctt ctggcagcgc gtgcacgcaa
     3961 ggacgggctg gtggtcgaga cacgcgtcat ggacgggcac gcgctccagt tcgctgacga
     4021 caccttcgac gtggccgggt cgcagttcgg cgtcatgctg tttccggaca tgcccaaagc
     4081 tgttcgcgag ttggtccggg tcgtcaagcc gggcggcagg gtgctcatca acgcgtacgg
     4141 cgaccctgag gagatcgaat tcctcggttt tctcgtgacc gcggtgcagt ccgtgcgccc
     4201 gggattcgat gggccaccgg cagaccctcc gccgctcgag ttccagctcg ccgaccccgg
     4261 caggctgcgg caagagctct ctgccgccgg actgaaggac gtgaaggtgg aagcaattac
     4321 cgagatcacg gagcatgcga ccgggaacga cctttgggaa tggctggtgt ggagcaaccc
     4381 tatcgtcgag tgcgtgctcg gcggcatgct gaacttgacc gaggacgagc gaggcgttgt
     4441 ccggcagaac ctggacaaat tggtgcatgc gcgcgccgcc ggcagccgtg ccgccaaact
     4501 taccaacccg gtcaatatcg gcgttggaac gaagtgaagt gaacagtacg aaggcgcccc
     4561 gcgaggcgcc tgccggaggg atatggcgaa gctccttgtc aacggcggtg tataacgatg
     4621 cagtgctgtc gttcctggca gatcagggat ccaccacggg atgtgcccca gcgccttgtg
     4681 cgaccccgac aatttctgtt agacgctcga gcccgaggcc atggccgacg cgaaacgcga
     4741 agacaagaaa cttcagtagc gttcgaacgc attctcgaaa aatgatggac gtcgctctgc
     4801 cataccgggt gcttgcggac gccgtgctcg tggcgcacgt gggcatcgtg ctattcgtcg
     4861 ttgccggctt cgttctggtc gttgccggca atgtgcgcca gtggcgctgg gtcaaccacc
     4921 tgtggttcag gctcgcgcat ctggccgcta tcgccgtggt ggtcgccgag tcgtggatag
     4981 gcatcacatg cccgctcacc acgctggaga tgtggctgcg ggcgcgagcc ggcgtgtcca
     5041 cctacagcga aggtttcgtc gagtactggc tgcagcgtgt cctctattac gatgcgcccc
     5101 cctgggtgtt cacgctgggc tatacgttgt tcgggttgct cgtcgcagcg acctggtggt
     5161 actttccgcc cgcgcgcagg agccgttgaa tcgctcgcgt ggaaggcaca cggggatcct
     5221 taccgcatcg cgggcttgac cagcctgacc cgcaatccat gttcgcgcct caaacccagc
     5281 ccatccatcc cagcaccgcg cccgcgccga gcagccagag caggtggatg cgggttcgcc
     5341 acaccagcag cgtcgacacg atcgcgacca gccagagccg ccagtcgcgc gcggcatcgt
     5401 agcggctgcc cgtcgccagg atccagccgg tggcgatgag cagcgcaatc acgatcggcg
     5461 tcatccccag cttgaacgcc cgcaccgaac gcaactcgcg gttcgcgtgg ctccagcgcg
     5521 cggtgacgta ggtgagcgtg gtgctgggga ccataatggc gaccatcgcg accgccatgc
     5581 ccagcagggc cgtcggcaag ccgcccgcgt tcaggccgac gttccatccg agcagcgcga
     5641 cgaacagcac gttgggcccg ggggcggcct gggcgatggc gattgaagcg ctgaactgcg
     5701 gatcggtgag ccagccctgg cgatcgacca gaaagcggtg catgtccggc gcggtcgtga
     5761 tggcgccgcc gacggacagc agcgagagcg ccaggaaatg cgttaagaac gagagccagt
     5821 cggcgggggt gatcgtgatc gtcatcgaat cgcctgggcg ggatgcaccg cgatgttcgc
     5881 cttgaaagcg gccgcgcgcc tcatgccccg gtccttccca tcttgcggta ggtcaagacg
     5941 catgacaggc cgcccagccc aagcagtacc caggccagcg gccagcgcag cagggcgatg
     6001 ccggcgaaag acgccacgcc gaacacgatg cacaggggca cgcccagcgg attctttctg
     6061 atcgcgccca gcagtttcat gcccgtggcc gtgatcagcc cggcggcgac cgcgcccatg
     6121 ccgcgcagcg cacccgcgac gttcaggttg tcggcgtact gcgcatagac cagcgccagg
     6181 cacagcacga ccaggagcgg gaacgtcagc atgccggcca gcgcggccat ggcgccgcgc
     6241 aagccgaaat agcggtcgcc gagcatcagt gcgagattga cgacattggg cccgggcatg
     6301 atctgggcga cggcccactc ctccacgaac tcgtcgcgcg tcagccagcg ctttttttcg
     6361 accagctcgc gctgtaccac ggcgagcacg cccccgaagc cttgcagcgc caggaaggtg
     6421 aacgatagaa acaggtcggt gagggagtgg ggttggggcc gctgttcgat ggatgttgtg
     6481 gcggccgctt cgggcgatga actcatggcg tcgattatca gcggctggcc attacgctgc
     6541 aggcactcgg gtaatggccg gccgcgttgc gagccacagg ccgccgccga tcagcgccag
     6601 cgcgcccgat gccgccggat gcagggcctc gccgagcaat gcccagccca gcagtgccgc
     6661 cgtcacggga ttgagcgcaa ggaacacggt gacgcgtgtc ggcgattcgt gcttgagcgc
     6721 atagagccaa cagaaatagc cgatggcgct ggacacgccg atgaagctca cggccgccca
     6781 ggcctggagg ctgaacgtga cgacgcgcgc cggccagtgc tcgcccaacg ccagcagcgc
     6841 aaggaacagc accgacgccg ccattgcgaa cgccgaaacg ggcaccgtcg gatagcgctc
     6901 cagataaggg cggtacagca cgctgcacag ggctccaatg aacgcggagg cggccaccgc
     6961 cagctcaccc caccaatgac cggactgtgc ctgcccaagc tttggggata gtgccagcgc
     7021 cacgccggcg atggagatca gcacccccag catcagcgat gccgtgacgc gctcgcggcc
     7081 cagcgccgcg gacatgagca gcgtgagcaa cggaaagagg ctgaagatca gcgccgcctg
     7141 ggccgctgct acgtgctgca gcccgtaatt gagcagggcg atcagcacac cgaactggcc
     7201 gatgcccagc gccgccatgg cgagcagatc gaccttccgg tttactgctg ccgaagtccg
     7261 gcgcgcttgc tgcgcactcg accacgcgcg ccacgcgaaa ggcaggagac agcaaaatcc
     7321 gatggcgtag cgcagcatcg ccaaagtgag gggcgggact tcaccgaccg cgaagcgaga
     7381 ggcgactatt cccgcgccca ccaggacgcc ggctgccgcc gccgcgagaa gagcgaggtt
     7441 tatggcttca gctcccacac ttcgctgacc atgttctgcc cgaa
//
LOCUS       DASWIY010000176         6619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1202389, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000176 DASWIY010000000
VERSION     DASWIY010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202389"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /gene="hslU"
                     /locus_tag="VGA59_06715"
     CDS             complement(<1..1173)
                     /gene="hslU"
                     /locus_tag="VGA59_06715"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX9719409.1"
                     /translation="MSSMTPQEIVSELDRHIVGQGDAKRAVAIALRNRWRRQQVDEKL
                     RGEITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTEVGYVGKDVDSIVRD
                     LAEMAVKQTRVAEMRKVRSRAEDSAEDRILDVLVPPPRTGEAGGEGTARQMFRKKLRE
                     GLLDDKDIEVELAESRAQLEIMGPAGMEEMTEQLRGMFSQFGGSKRKTRKLKIVEAMK
                     LLVEEEAAKLVNEDEIKTAAIHNAEQNGIVFIDEIDKVASRGFGGEGGGGGADVSRQG
                     VQRDLLPLVEGTTVSTKYGPVRTDHVLFIASGAFHLSKPSDLIPELQGRLPIRVELKS
                     LSVQDFEAILTQTHASLVKQYQALLATEGVTLEFQPEAITRLANIAFEVNERTENIG"
     gene            complement(1293..1886)
                     /gene="hslV"
                     /locus_tag="VGA59_06720"
     CDS             complement(1293..1886)
                     /gene="hslV"
                     /locus_tag="VGA59_06720"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX9719410.1"
                     /translation="METFHGTTIVSVRRKDAAGHWQVAIGGDGQVTLGNVVVKGTARK
                     VRKLYHDKVVAGFAGATADAFTLFERFEAKLEKHQGHLVRAAIELTKDWRTDRVLRRL
                     EAMLAVADAEASLIITGSGDVLEPEHGIIAIGSGGAYAQAAAKALLDNTELGASDIVK
                     KSLEIAGELCIYTNMHHTIEVLGEETRDEPKGLGTRG"
     gene            complement(1996..3879)
                     /locus_tag="VGA59_06725"
     CDS             complement(1996..3879)
                     /locus_tag="VGA59_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719411.1"
                     /translation="MATKEENSGLLSKVVKFVRNPTTNWADLDQQETDRESSYSKQML
                     KEMIERKRRNDFVRKREFDMLRKMRRSEVMAGQDPAARPSFFQSSMPSKPDDRATTLK
                     KIDEIEAQMSMQWWKTKHGDVSSRSGSSTNFPMSSHVPPEAIGRAVGAPASPLGSTAY
                     TRTEPQPLRPDKDAETTAPDRLEPLLPASAAAAPSGMAPLTPEAGPVPVTVEPAGAAA
                     PSVLPDAPAVAAGMRAGPPSEQSSSSIGFSASKLFAIDVEEFAHDPELEEASIRFANG
                     DDAGAEAGLMEVLGPAGSRVDHDETWLTLFDLYRATGQQDRFETAAIDFAGRFGRSAP
                     QWFSVPEAVGRMHAPAAALEGPTQRPANWTCPATLGTQTVAAMAVALAKAQPPWRMSW
                     AKLTTIDEAAVEGLTKLFTKWAGQPVQLRFIGADKFEKVLKDATPSGDKGVNPAWWKL
                     RMEVLRVMHRPDEFELVALDYCVTYEVSPPSWDSARCEYKPLQADGSFVPGHTIVGDA
                     FRDSLSSGMSMGYSDTHAVGQSSQMSNISTVELAGQVLGDATEAMEMLENKLMGADVM
                     IISCARLIRIDFTAAGSLLNWVTSRQVEGRQVQFVEVHRLVAAFFNVIGISEHARVLA
                     RTD"
     gene            complement(3923..4945)
                     /gene="dksA"
                     /locus_tag="VGA59_06730"
     CDS             complement(3923..4945)
                     /gene="dksA"
                     /locus_tag="VGA59_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX9719412.1"
                     /translation="MKPQTKGSVKAAKPAVKAKPAAKAKPVAKSAAKKPAAGKLPARS
                     ATKAAPARKASAKPAAKTPARKIPAKTAKPVAKKAVPVPKSKPAPKAAATKHKAAPAK
                     AAPAKASPAKAAAKSKPAGPALPRAMPPRPDAATLTAEPARATKPSARLAQITVPSMA
                     QSVASTAAKASYIQTMSPSVLTPPPLLAVKKDPKLANNWKTKPVEELTDAEVMAMPDS
                     EYMNEKQMAFFRFKLTQLKQEILNSAGETTEHLREDTVIVPDPADRATIEEEHALELR
                     TRDRERKLLKKIEQSIARIDAGDYGFCDETGEPIGVGRLLARPTATLSLEAQQRRELK
                     QKMFGD"
     gene            complement(5197..6240)
                     /locus_tag="VGA59_06735"
     CDS             complement(5197..6240)
                     /locus_tag="VGA59_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9719413.1"
                     /translation="MSLIPATILTGFLGSGKTTLLKRVLEEAHGQKIAVIENEFGEEN
                     IDSDILVSDTREQIIQMSNGCICCTIREDLRATLQLLAAKKRKGLLEFDRVVIETTGL
                     ADPGPVAQTFFMDDEIAETYLLDSILTLVDAKHAGGQLDDRQEARRQVGFADQIFISK
                     TDLVAQDETDALIHRLRHMNPRAPQKAVHFGQVQLSEVFDLRGFNLNAKLDIDPDFLQ
                     DEEASHDHDHEHGEHCDHPSHEGQGHHHHHDDDVKSFVFKSDRAFDPARLEDFLGAVV
                     NIYGPRMLRYKGVLHMRGTDRKVIFQGVHQLMGSDLGPLWADAEKRGSKMVFIGIDLP
                     KDIFLQGLEQCLV"
     gene            complement(6343..>6619)
                     /locus_tag="VGA59_06740"
     CDS             complement(6343..>6619)
                     /locus_tag="VGA59_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593212.1"
                     /note="SAM-dependent;catalyzes the methylation of cytosine
                     at position 1962 of the 23S rRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HEX9719414.1"
                     /translation="TAAHADRAARAYKDINRLALKLLEPGGVLFTYSCSGAIGADLFH
                     KIVASAGADAGVDGYIGERLGGAPDHPMTLAFPEGEYLKGLVVVRKG"
BASE COUNT          973 a         2156 c         2175 g         1315 t
ORIGIN      
        1 cccgatgttt tcggtgcgct cgttcacctc gaacgcaatg ttcgccagcc gcgtgatggc
       61 ctcgggctgg aattcgagcg tgacgccttc cgtggcgagc aaggcctggt actgcttgac
      121 cagcgaagcg tgggtctgcg tcaggatcgc ctcgaagtcc tgcaccgaca gcgatttgag
      181 ttcgacgcgg atcggcaggc ggccctgcag ttcggggatc aggtcgctgg gcttggacag
      241 gtggaaggcg ccgctcgcga tgaagaggac gtgatcggtc ctgaccggtc catacttggt
      301 ggagacggtt gttccctcga ccaggggcag caggtcgcgc tgcacgccct ggcgcgaaac
      361 gtcggctccg ccgccgccgc cttccccacc gaagccgcgt gaggcgacct tgtcgatctc
      421 gtcgatgaag acgatcccgt tctgctcggc gttgtggatg gcggcggtct tgatttcgtc
      481 ctcgttcacg agcttggcgg cttcttcttc gaccagaagc ttcatcgcct cgacgatctt
      541 gagcttgcgc gtcttgcgct tggagccgcc gaactggctg aacatgcccc gcagctgctc
      601 ggtcatctcc tccatgcccg cgggccccat gatctcgagc tgcgcgcgcg actccgccag
      661 ttcgacctcg atatccttgt cgtcgagcag gccctcgcgc agcttcttgc ggaacatctg
      721 ccgcgcggtg ccctcacccc cggcctcacc cgtgcgcggc ggcggcacca gcacgtccag
      781 gatgcgatct tccgccgaat cttccgcgcg cgaacgcacc ttgcgcatct cggcgacgcg
      841 cgtctgcttg acggccatct cggccaggtc gcgaacgatg gaatccacgt ccttgccgac
      901 gtagcccact tcggtgaact tggtcgcctc gaccttgatg aaaggcgcgt cggccaggcg
      961 cgccagtcgc cgcgcgatct cggtcttgcc cacaccggtc gggccgatca tcaggatgtt
     1021 cttcggcgtg atttcaccgc gcagcttttc gtccacctgc tgccggcgcc agcggttgcg
     1081 caaggcgatg gcgacggcgc gtttggcgtc gccctggccg acgatgtgcc ggtccagttc
     1141 ggaaacgatt tcctgggggg tcattgagga cataggggct aggggctagg ggctaggggg
     1201 ggtggggcct gcaggtggag ggctaaggct gcggacgaag gcatggagct gaggcaagag
     1261 gccggtcttg gctgttccct agtccctagt ccctagcccc tagtccctag ccccttcggc
     1321 tcgtccctgg tctcttcccc aagcacctcg atcgtgtgat gcatattcgt atagatgcac
     1381 agctcgccgg cgatctccag cgacttcttc acgatgtcgc tcgcgcccag ctcggtgttg
     1441 tcgagcaggg ccttggccgc cgcctgcgca taggcgccgc ccgagccaat ggcgatgatg
     1501 ccgtgctcgg gctccagcac atcgccgctg ccggtgatga tgagggatgc ctccgcgtcg
     1561 gcaacggcca gcatggcttc cagccggcgc agcacccggt cggtgcgcca gtccttggtc
     1621 agctcgatcg ccgcgcgcac caggtgcccc tggtgctttt ccagcttggc ttcgaagcgc
     1681 tcgaacagcg tgaatgcatc cgcggtggcg cccgcgaatc cggcgaccac cttgtcatgg
     1741 tagagcttgc gcaccttgcg ggccgtcccc ttgaccacga cgttgcccag cgtcacctgg
     1801 ccgtcgccgc cgatcgccac ctgccaatgg cccgcggcat ccttgcgccg cacgctgacg
     1861 atggtcgtgc cgtgaaaagt ttccataggg gctaggggct agggaattag ggattagtgg
     1921 tcgaagactc gggccgataa cgtgaatata ggggctgttc ccctaatccc tagcccctaa
     1981 cccctagtcc ctgtattaat cagtccgcgc cagaacccgg gcgtgctcgc tgatgccgat
     2041 gacgttgaaa aacgccgcca ccagccggtg cacttcgacg aactggacct ggcggccttc
     2101 gacctggcgg gacgtcaccc aattgagcag actgcccgcc gccgtgaaat cgatgcggat
     2161 caggcgcgcg caggaaatga tcatgacgtc cgcgcccatc agcttgtttt ccagcatctc
     2221 catcgcttcg gtggcatccc caaggacctg cccggccagc tcgaccgtgg aaatattgga
     2281 catttgcgag ctctgcccca cggcatgggt atcgctatag cccatgctca tgcccgagga
     2341 caacgaatcg cggaatgcgt cgcccacgat cgtgtggccc ggcacaaaac tgccgtccgc
     2401 ctgcaacggt ttgtactcgc agcgcgcgct gtcccacgat ggcggcgaga cctcgtaggt
     2461 gacgcaatag tccagcgcca ccagttcgaa ctcgtccggg cggtgcatca cgcgcagcac
     2521 ttccatgcga agcttccacc aagctggatt cacgcccttg tcgcccgacg gggtggcgtc
     2581 cttgagcact ttctcgaact tgtcggcgcc gatgaagcgc agctgaaccg gctggcccgc
     2641 ccatttcgta aaaagcttcg tcaggccctc caccgcggct tcatcgatgg tggtgagctt
     2701 ggcccagctc atgcgccagg gtggctgggc cttggccagc gcgaccgcca tcgccgccac
     2761 agtctgggtg cccagcgtcg cggggcaggt ccagttcgcc ggccgctggg tggggccttc
     2821 cagcgcggcc gcgggagcat gcatccggcc gacggcttcc ggcacggaaa accactgcgg
     2881 cgcggagcgg ccgaatcggc cggcgaagtc gatcgcggcg gtttcgaacc ggtcctgctg
     2941 cccggtggca cggtacaggt cgaacagcgt cagccaggtc tcgtcatggt cgacgcggga
     3001 gcccgcgggc ccgagcacct ccatcagccc cgcttcggcg ccggcatcgt cgccgttggc
     3061 gaagcggatc gaggcctctt cgagctcggg gtcgtgcgcg aattcttcca cgtcgatggc
     3121 gaacagcttg gaagcggaaa aaccgatgct gctgctcgac tgttcggaag gcgggccggc
     3181 acgcatgccc gccgcaaccg cgggagcgtc cggtagaacg gacggcgcag cggcgcctgc
     3241 cggttcgaca gtaaccggca ccggcccagc ctccggcgtg aggggcgcca tgccactggg
     3301 cgcggccgcg gcagacgcgg gcagcagcgg ctcgagccgg tccggcgccg tggtttccgc
     3361 atccttgtcc gggcgcagcg gctggggctc cgtgcgggtg taggccgtgg acccgagcgg
     3421 ggacgcgggc gcgcccacgg cgcggccgat cgcttcgggc ggcacatggg acgacatggg
     3481 gaagttggtg ctgctgccgc tgcggctgga gacgtcgccg tgcttggtct tccaccattg
     3541 catcgacatc tgggcctcga tctcgtcgat tttcttgagc gtggtggccc ggtcgtccgg
     3601 cttggacggc atggagctct ggaaaaacga gggccgggcc gccgggtcct gcccggccat
     3661 cacctcgctg cggcgcatct tgcgaagcat gtcgaattcg cgcttgcgga cgaaatcgtt
     3721 gcgccgcttg cgctcgatca tctccttgag catctgcttg gagtagctgc tctcgcggtc
     3781 ggtctcctgc tggtcgagat cagcccagtt cgtggtcggg ttcctgacga acttcaccac
     3841 cttcgaaagc aatccgctgt tttcttcctt cgtcgccatg ggggagctgt tacggtgtac
     3901 tgcaggaagg ggattgccgg ttttagtccc cgaacatttt ctgcttgagc tcgcggcgct
     3961 gctgggcttc cagcgacagc gtggcggtgg gccgcgccag cagccgcccc acgccgatcg
     4021 gctcgccggt ctcatcgcaa aagccatagt cgccggcgtc gatgcgggcg atcgactgct
     4081 cgattttctt gagcagcttt cgctcgcggt ctcgggtgcg cagttccagc gcatgttctt
     4141 cctcgatcgt ggcccggtcg gccggatccg gaacgatcac ggtgtcctcg cgcaggtgct
     4201 ccgtcgtctc ccccgcgctg ttgaggatct cctgcttgag ctgcgtgagc ttgaagcgga
     4261 agaacgccat ctgcttctcg ttcatgtact cggagtccgg catggccatg acttccgcat
     4321 ccgtcagctc ttcgaccggc ttggtcttcc agttgttggc gagcttgggg tctttcttga
     4381 cggccaacag gggcgggggg gtaaggactg aaggggacat cgtctggata taacttgctt
     4441 tggcggcggt cgacgccacg gattgcgcca tcgagggcac cgtgatctgc gccagccgcg
     4501 ccgagggttt ggtggcccgt gccggttcgg ccgtcagggt ggcggcatcg ggacggggcg
     4561 gcatggcgcg cggcagcgcg ggccctgctg gtttggactt ggcggccgcc ttggcagggg
     4621 acgccttggc gggagccgcc ttggcgggag ccgccttgtg cttggtcgcc gcagccttgg
     4681 gggccggttt ggacttgggt accggcaccg ccttcttcgc gaccggcttg gctgtcttgg
     4741 caggtatttt cctggccggc gtcttggcgg ccggctttgc gcttgctttc ctggccggcg
     4801 ccgctttggt cgcggacctg gccgggagct tcccggccgc cggcttcttg gccgcggact
     4861 ttgccaccgg cttggccttc gccgccggct tcgccttgac ggctggcttg gccgccttca
     4921 cgctcccctt cgtttgaggc ttcacttcct gtctcctcgc ggcagcagcg ccggcatgga
     4981 tttgccgcgc acgcgccgac cttccttgtt ctgatggatc gccttcgttt tcatgaatgc
     5041 gagttatacc ccgattgtcg ggacccgggc gctcgctgtt ggggttttca ccccggcgct
     5101 caggcgcggg cccgccaaag ggtagaccca tcagttgcag aatcgagaca aacattcggg
     5161 ctcgcagctc agttacggaa aacgcgctag cctacctcaa accaggcact gttcgagccc
     5221 ttgcaggaag atatctttag gtagatcaat tccgatgaag accatcttgc tgccccgctt
     5281 ctcggcgtcg gcccagagcg gccccaggtc gctgcccatc aactggtgca cgccctggaa
     5341 gatcaccttg cggtcggtgc ccctcatgtg cagcacgccc ttgtaacgca gcatgcgcgg
     5401 cccgtagata ttgacgacgg cacccaggaa atcctccagc ctggccggat cgaacgcgcg
     5461 gtccgacttg aacacgaagc tcttgacgtc gtcgtcgtgg tgatggtggt ggccttggcc
     5521 ctcatgggag gggtggtcgc agtgctcgcc atgctcatgg tcgtggtcgt gggaagcctc
     5581 ttcgtcctgc aggaagtcgg gatcgatgtc aagcttggcg ttgaggttga agccgcgcag
     5641 gtcgaacacc tcggacagct gcacctggcc gaaatgcacc gccttttgcg gcgcgcgcgg
     5701 gttcatgtgc ctcaggcggt ggatcagggc atcggtctcg tcctgggcca ccaggtcggt
     5761 cttgctgatg aagatctggt cggcgaaacc cacctggcgg cgcgcctcct gccggtcgtc
     5821 gagctgcccg ccggcgtgct ttgcatcaac cagggtcagg atcgagtcga gcaggtaggt
     5881 ttcggcgatc tcgtcgtcca tgaagaaggt ctgcgccacc gggccggggt cggccaggcc
     5941 cgtggtctcg atcaccacgc ggtcgaactc cagcaggcct ttgcgcttct tggcggccag
     6001 cagctgcagg gtggcgcgca gatcctcgcg gatggtgcag cagatgcacc cgttgctcat
     6061 ctggatgatc tgctccctgg tgtcgctgac caggatgtcg ctgtcgatgt tttcctcgcc
     6121 gaattcgttc tcgatcacgg cgatcttctg gccatgggcc tcctcgagca cgcgcttgag
     6181 cagggtggtc ttaccggagc cgagaaagcc cgtgaggatg gtggcgggga tgagactcat
     6241 gttgtaactc cagggagggc ccccgagttt aaggaggttg ggcgtcgggc gctgggcctc
     6301 gagggagaaa acccctgtgt ttcccgctga acgctcaacc ctttaacctt tccgaaccac
     6361 caccagcccc ttcaggtact ccccttccgg gaacgccagc gtcatcgggt ggtcgggcgc
     6421 gccgcccagc cgctcgccga tgtagccgtc caccccggca tcggcgccgg ccgaagcgac
     6481 gatcttgtgg aacaggtcgg cgccgatcgc gccggagcag gagtaggtga acagcacgcc
     6541 gcccggctcc agcagcttga gcgccagccg gttgatgtct ttgtacgccc gggccgcgcg
     6601 atcggcgtgg gccgccgtg
//
LOCUS       DASWIY010000177         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_471931, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000177 DASWIY010000000
VERSION     DASWIY010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471931"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1021
                     /locus_tag="VGA59_06745"
     CDS             101..1021
                     /locus_tag="VGA59_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9719415.1"
                     /translation="MPEPIFRIANLYKKFGDNTVVNDLSLEIRRGECLGVIGPNGAGK
                     TTTLRMCLGQAAPDAGHIEAFGLPIPARVREAKQRIGVVTQFDSLDPDFSCAENLRVY
                     GRYFGLPMATIDARIPKLLEFAALQSKAQAKPGELSGGMRRRLSLGRALINDPDLLLL
                     DEPTTGLDPQARHLMWERLKALLQQGKSILLTTHFMDEAERLCDRLIVLDHGRKIAEG
                     TPRGLIREHLESDVVEVYGDGAQAVMLAHNGQAQRVETSGETVFFYLHEPRPLLQLLS
                     ADHSVRYLHRPANLEDLFLKLTGRQIRDDA"
     gene            1034..>1690
                     /locus_tag="VGA59_06750"
     CDS             1034..>1690
                     /locus_tag="VGA59_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9719416.1"
                     /translation="MLISLYAAPRITLRFVPVVMRNLLVWRKLAAASIVGNIAEPLIT
                     LVAFGYGLGSMIGVVAGLPYITYLASGSVAASAALAATFEALYSAYSRMQVQKTWESI
                     MNAPFALDDIVFAEMLWAAMKSLFSVGAIMVVIYALNISRSPGMLLALPLLGLVGVTF
                     ASIALVFNALAKGYDFFTYYFTLVLTPMTFLSGVYFPISQMPAWLQSVAQVLPLTAAV
                     K"
BASE COUNT          273 a          602 c          531 g          284 t
ORIGIN      
        1 cgcccaaggg ccagcacgac gctggccctt tgcgcttgag gcgatgcccc atggcccaca
       61 gcccggcgca gacgacccag ctttggcaca ctgccttccc atgcccgagc cgatcttccg
      121 cattgccaac ctgtacaaga agttcggcga caacaccgtc gtcaacgact tgtcgctgga
      181 gatccggcgc ggcgaatgcc tgggcgtgat cggccccaac ggcgcgggca agaccaccac
      241 cctgcgcatg tgcctcgggc aggccgcgcc cgatgccggc cacatcgagg ccttcggcct
      301 gccgattccc gcgcgcgtgc gtgaggccaa gcagcgcatt ggcgtcgtga cgcagttcga
      361 cagcctggac ccggacttca gctgcgccga aaacctgcgc gtctacggcc gctacttcgg
      421 tctgccgatg gcgaccatcg acgcgcgcat ccccaagctg ctggagttcg cggcgctgca
      481 atccaaggcg caggccaagc cgggcgagtt gtccggcggc atgcggcggc gcctgagcct
      541 ggggcgcgcg ctcatcaacg accccgatct gctgctgctg gacgaaccca ccaccgggct
      601 ggacccgcag gcacgccacc tgatgtggga acgcctgaag gccctgctcc agcaaggcaa
      661 gtccatcctg ctgaccaccc acttcatgga cgaggccgag cgactgtgcg accgcctcat
      721 cgtgctggac cacggccgca agatcgccga aggcacgccg cgcgggctga tccgcgagca
      781 cctggaaagc gacgtggtcg aggtctacgg tgacggtgcc caggccgtga tgttggccca
      841 caacggccag gcccagcggg tggagaccag cggcgaaacc gtgttcttct acctgcacga
      901 gccgcgcccg ctgctgcagt tgctgagcgc cgaccacagc gtgcgctacc tgcaccggcc
      961 ggccaacctg gaagacctgt ttctcaagct caccggccgc cagatccgcg acgacgccta
     1021 gatcggaaac gccatgctga tttcgctcta cgccgccccc cgcatcaccc tgcgcttcgt
     1081 gccggtggtg atgcgcaact tgctggtgtg gcgcaagctc gcggcggcca gcatcgtggg
     1141 caacatcgcc gagccgctca tcaccctggt ggccttcggc tacgggctgg gttcgatgat
     1201 cggcgtggtg gccggcctgc cctacatcac ctacctcgcc tcgggcagcg tggcggcgag
     1261 cgcggcgctg gcggccacct tcgaggcgct gtactccgcc tactcgcgca tgcaggtgca
     1321 aaagacctgg gagagcatca tgaacgcgcc cttcgcgctc gacgacatcg tgttcgccga
     1381 gatgctgtgg gcggcgatga agagcctgtt ttcggtcggc gccatcatgg tggtcatcta
     1441 cgcactcaac atcagccgct caccgggcat gctgctggcg ctgccgctgc tgggcctggt
     1501 cggcgtcacc ttcgcttcca tcgcgctggt gttcaacgcg ctggccaagg gctacgactt
     1561 cttcacctac tacttcaccc tggtgctcac gcccatgacc ttcttgtcgg gcgtctactt
     1621 tcccatcagc cagatgccgg cgtggctgca atcggtggcg caggtgctgc cgctgaccgc
     1681 ggcggtgaaa
//
LOCUS       DASWIY010000178         3637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1585984, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000178 DASWIY010000000
VERSION     DASWIY010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585984"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VGA59_06755"
     CDS             complement(<1..249)
                     /locus_tag="VGA59_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX9719417.1"
                     /translation="MTARTDLWTLAVIVGLAGVTVLTRCFFFILDRPWSLPGWAERAL
                     QYAPVAALAGVIAPEVVMSSGHLVATWQDARLFAALAGT"
     gene            complement(272..949)
                     /locus_tag="VGA59_06760"
     CDS             complement(272..949)
                     /locus_tag="VGA59_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX9719418.1"
                     /translation="MAPMAAGIAAWGLMTGVAMVKSGMSVVEALAMTFLVYAGSSQLA
                     AIPLIVAAAPAWVILATGFCVNLRFVVFSLHLRPYLMHMPRWRRLVNGYMTADLSYAL
                     FTRRFPRPGESHAQRAEQEAYLAGNYCVTWFGWMMPSVAGIGLANLIPSAWGLGFAGV
                     LCLVGVLCSLANTPLRRLSALLAGVAAVAAYALPLKLNIVVAIAVAVLASFWLEKKLT
                     PAARQPA"
     gene            complement(1006..2013)
                     /gene="fmt"
                     /locus_tag="VGA59_06765"
     CDS             complement(1006..2013)
                     /gene="fmt"
                     /locus_tag="VGA59_06765"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX9719419.1"
                     /translation="MRVIFAGTPEFARVALERLHGAGHAIALVLTQPDRPAGRGMKLQ
                     PSPVKQFALAWQVPVAQPRSLRLDGKYPEDALAAKAAIEQARADVMVVAAYGLILPQW
                     VLDAPRLGCLNIHASLLPRWRGAAPIHRAIEAGDRETGVTIMQMDAGLDTGAMLLAGR
                     IPIDAGDNTGTLHDKLAALGGALVARALAENGRLQPMAQPEAGVTYAHKVDKAEGIID
                     WLQPAEVIARRIRAFDPFPGASSALAGEQIKLWRGQAVTDGAGHGPPGTIVAVGAAGI
                     DVRCGQGALRVTQLQRAGGKRLAAADFLHGFELKPGMMFEPAPVHAALPGAHVAKTAP
                     M"
     gene            complement(2024..2527)
                     /locus_tag="VGA59_06770"
     CDS             complement(2024..2527)
                     /locus_tag="VGA59_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719420.1"
                     /translation="MSALRKLLLACIAAPLIGCAGNPFAGYDIPPGTPRDAVIARMGQ
                     PHRVVHLVTGERLQYSLQPFGQYAWMVDVDATGRVIQARQVLTDNNFHRIEPGKWTRE
                     DVEREFGRPAWVDGVASWRGPIMTYRWRDLGGGDMFYWIYLDERNVVQRAHPGMEFIN
                     APDDPRN"
     gene            complement(2554..3066)
                     /gene="def"
                     /locus_tag="VGA59_06775"
     CDS             complement(2554..3066)
                     /gene="def"
                     /locus_tag="VGA59_06775"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX9719421.1"
                     /translation="MALLPLLYYPDPKLHTVAKPVQAVDARIKQLIGDMLETMYEANG
                     IGLAATQVDVHERLIVIDVSEQRNSPLVLINPEIPWASQEKQLSDEGCLSVPGVYDGV
                     ERAAAIRATALDGEGRPRTIEAEGVLAVCIQHEMDHLLGKVFVEYLSPLKRNRIKTKM
                     LKARRETVRA"
     gene            3387..>3637
                     /locus_tag="VGA59_06780"
     CDS             3387..>3637
                     /locus_tag="VGA59_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX9719422.1"
                     /translation="MIGSLTASPALAQNFPVTPGQRSTAEQVAQAGVPLSELAPNAPE
                     EYTVKRGDTLWAISGLFLKSPWRWPELWGMNMQEVRNPHL"
BASE COUNT          619 a         1236 c         1201 g          581 t
ORIGIN      
        1 cgtcccggcc agcgcggcga acaggcgcgc atcttgccag gtcgccacga gatggccgct
       61 ggacatgacc acctcagggg cgatcactcc cgccaatgcc gcgaccggcg cgtactgcag
      121 ggcgcgctca gcccagccgg gcaggctcca gggacggtcc aggatgaaga agaagcagcg
      181 ggtgagcacc gtcacgccgg ccagacccac gatcacggcc agcgtccaca gatcggttcg
      241 cgcggtcatg gggaaacctc cgtgccgggc ctcatgcggg ctgcctcgct gccggcgtca
      301 acttcttttc cagccagaag ctggccagca cagccacggc gatcgccacc acgatgttca
      361 gcttgagcgg caacgcatac gcggccaccg ctgccacgcc ggccagcagc gccgacagcc
      421 gccgcagcgg ggtgttggcg agcgagcaga gaacgccgac gaggcagagg acgccggcaa
      481 aacccagacc ccaggcgctg gggatcaggt tggccaagcc gatcccggcg acgctcggca
      541 tcatccagcc gaaccaggtg acgcagtagt taccggccag gtacgcctcc tgctcggcac
      601 gttgtgcgtg actttcgccc gggcgcggaa aacggcgggt gaacagcgcg tagctcaggt
      661 ccgcggtcat gtagccgttc accagccgcc gccagcgcgg catatgcatc aggtagggtc
      721 tcaggtgcag gctgaacacg acgaaccgca ggttcacgca gaagccggtg gccaggatca
      781 cccaggccgg ggccgcagcg acgatcagcg ggatggcggc gagctgcgaa ctgcccgcgt
      841 agaccaggaa tgtcatggcc agcgcctcga cgacgctcat gccggacttg accatggcca
      901 caccggtcat cagaccccag gcggcaatgc cggcggccat gggcgccatg tctcgcgcgc
      961 cttcgcgaaa tgccgggtgg cggtaattgg aacgaaggaa gaacactaca taggtgccgt
     1021 cttggcgacg tgcgcaccgg gtaaggcggc gtgaacgggc gcaggctcga acatcatgcc
     1081 gggcttcagc tcgaagccgt gcaggaagtc tgccgcagcc agccgcttgc cgccggcgcg
     1141 ttgcagctgc gtgacgcgca aggccccctg cccgcagcgc acgtcgatgc cggccgcacc
     1201 caccgccaca atcgttccgg gcggtccatg gccggccccg tcggtgacgg cctgcccgcg
     1261 ccacaacttg atctgctcgc cagccagtgc gctgctggca cccggaaacg ggtcgaaggc
     1321 acggatgcgc ctggcgatca cctcggccgg ttgcaaccaa tcgatgatcc cctcggcctt
     1381 gtccaccttg tgagcgtagg tgacgccggc ctcgggttgc gccatgggct gcaggcggcc
     1441 gttttcggcc agcgcccggg caaccagcgc gccaccgagc gccgccagct tgtcgtgcag
     1501 cgtgccggtg ttgtcacccg catcgatggg aatccgcccg gccagcagca tggcgccggt
     1561 gtcgaggccg gcatccattt gcatgatcgt cacgccggtc tcgcggtcgc ccgcttcgat
     1621 cgcacggtgg atcggcgccg cgccgcgcca gcgcggcagc aaggaagcat ggatgttgag
     1681 acaacccagg cgcggggcat ccagcaccca ctggggcagg atcaggccat aggcagccac
     1741 gaccatcacg tcggcgcgcg cctgctcgat cgcggccttc gcggccagtg cgtcctcggg
     1801 atacttgccg tccaggcgca agctgcgcgg ctgcgcgact gggacttgcc acgccagcgc
     1861 gaattgcttg accggcgacg gctgcagctt catgccgcgg ccggccggcc gatcgggctg
     1921 ggtcaggacc agcgcaatgg cgtggccggc gccgtgcagg cgctccagcg ccactcgggc
     1981 gaattccggg gttccggcga agatgacgcg cacgctgatg cagtcagttc cgggggtcgt
     2041 cgggggcgtt gatgaattcc atgccgggat gggcgcgctg caccacgttg cgctcgtcca
     2101 ggtagatcca gtagaacata tcgcccccac ccaggtcccg ccagcggtag gtcatgatcg
     2161 ggcccctcca gctggcaacg ccgtcgaccc acgccggccg gccgaattcg cgctcgacgt
     2221 cctcgcgcgt ccatttgccc ggctcgatgc ggtggaagtt gttgtcggtg agcacttgcc
     2281 gggcctggat caccctgccc gttgcgtcga cgtccaccat ccaggcgtac tggccgaatg
     2341 gctgcagcga gtattgcagc cgctcgccgg tgaccaggtg caccacccgg tgcggctggc
     2401 ccatgcgtgc aatcacggca tcgcgcggcg tgcccggtgg aatgtcatag ccggcgaaag
     2461 gattgccggc gcatccgatc agcggcgccg caatgcaggc cagcagcagt ttgcgcaatg
     2521 cggacacggg gcgttctcct ggctgagtgg cgttcaggcg cggacggttt cccgccgcgc
     2581 cttgagcatc ttcgtcttga tgcggttgcg cttgagcggc gacaagtatt ccacgaacac
     2641 cttgcccagc aggtggtcca tctcgtgctg gatgcagaca gcgagaacgc cctcggcttc
     2701 gatcgtccgc ggccgtcctt cgccatcgag cgcggtcgcc cgaatcgccg ccgcgcgttc
     2761 gacaccatcg tagacgcccg gcaccgacag gcagccttcg tcgctgagct gcttctcctg
     2821 gctggcccag gggatctccg ggttgatcag caccagaggc gagttgcgct gctcggacac
     2881 gtcgatcacg atcaggcgtt cgtgcacgtc cacctgcgtc gccgccagtc cgatgccgtt
     2941 ggcctcgtac atggtctcga gcatgtcgcc gatcaactgc ttgatgcgcg cgtccacagc
     3001 ctgcaccggt ttggccaccg tgtgcagttt ggggtcaggg taataaagaa ggggaagaag
     3061 tgccatgggt ttaaggttgg ccccgtactt cgctgcttca aggggggcaa tttttccttg
     3121 aggcgcgccg gcgtgaaaaa gccgaaggtg tcgctatttt cgctactttt gcctttccag
     3181 gcagcgcaca aatgccataa acgttgccca atcaagggct tgagcgcaga atcgccagta
     3241 ctttgtcaag actttcgact gccatcggcc cgccgacagt cgcagcccta atgcacaaaa
     3301 acagccacac cccgatgacg gttcgctcca agtcttctgg tccgggcact cccggcgtcc
     3361 tgagcgcctg ggcagtgctg gcggccatga tcggtagcct gacggcgtcg ccggccctcg
     3421 cgcagaactt cccggtgacg ccgggacagc gctccacggc cgagcaggtg gcccaagccg
     3481 gcgtgccgct gtcggaactc gcgcccaacg ccccggagga atataccgtc aagcgcggcg
     3541 ataccctgtg ggcgatttcc ggcctgttcc tcaagagccc ctggcgctgg ccggaactgt
     3601 ggggcatgaa catgcaggag gtgcgcaatc cgcacct
//
LOCUS       DASWIY010000179         7012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1203938, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000179 DASWIY010000000
VERSION     DASWIY010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203938"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VGA59_06785"
     CDS             <1..222
                     /locus_tag="VGA59_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX9719423.1"
                     /translation="ADVALHKSRYFGSAWANYESARPGSLKLVPPGYRWGAIEADYVK
                     MRPMFLDEPAPFAELMHQVAGAEKALNAL"
     gene            228..3659
                     /locus_tag="VGA59_06790"
     CDS             228..3659
                     /locus_tag="VGA59_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX9719424.1"
                     /translation="MTASHSNFAFLQTEWPALHAEALRAESSLATDPRAACFYARRTL
                     ELAVAWLFRADPALKMPYKADLSAFLFEPSFKVLVGPALHAKMDVIRKQGNSAVHSAR
                     PVQPADALGVLRELFQVAFWLARHYGRGAGARPDPALQFRADLLPKADAAAAAQTLAA
                     LQKLAQDLAERDATVATAQKKAAALDEELARLRAEVAAAKAVNNAQPDAHDYNEAQTR
                     DLYIDLLLKEAGWALDQTRDREFEVAGMPNNQGQGFVDYVLWGDDGKPLAVVEAKRTL
                     KDSRVGQQQAKLYADCLQAQFGQRPLIYYTNGYEHWFWDDSRYPPRPVQGFHKKDELQ
                     LLMQRRGSRKPLAAAPIDGAIVERHYQHRAIRRIAETFEQDKQRKALVVMATGAGKTR
                     MVIALVDMLMRGNWAKRVLFLADRVALVNQAVNAFKAHLPDAAPVNLVTDKYGEGRVY
                     VSTYPTMMGLINETNDQQRRFGVGHFDLIVVDEAHRSIYQKYRAIFSYFDAFLVGLTA
                     TPKDEIDRNTYSLFDLENGVPTDAYGLDDAIAEGYLVPPRAVSVPLKFQREGIKYSDL
                     SEEDREQWEALDWDDEGGIPEEVNADAVNKWLFNKDTVDKVLETLMTRGQKVAGGDRL
                     GKTIVFAKNNDHAEFIAERFNANYPHYAGLFARVITFKTEYAQSLIDDFSVKDKSPHI
                     AVSVDMLDTGIDVPEVLNLVFFKIVRSKTKFWQMVGRGTRLCKDLFGPGQDKRDFLIF
                     DFCQNLEYFSQDLPTTEGAVGQPLGQRLFNARLELIAALDQRCAASGRQAAEGSALGG
                     VRLTEESIRSETAGMLHRIVAGMSLDNFVVRPKRKWVQAWAHGSAWNRLGADQLQEIS
                     EHLSGLPTAVRDDDEEAKRFDLLMLRLQLCMLRAEPGFERLQQQVREIADRLSELGSI
                     PAVREQMLLIDAVAGEEWWKDVTIPMLEQARRHLRVLVKLLDKSGRKVVYTDFEDELG
                     ESTDVELPVGGSAGNFERFRVKARAFLRAHEDHIALHKLRRNQALTPSDLAELERMLA
                     ESGVGTAQDIEQAKQEAQGLGLFIRSLVGLDRQAATEALAVFLADKTLSGNQIEFVNM
                     MVNHLTEHGVMKPELLYESPFTAYAPQGPDRLFSSAQVDELFRVLEQVRATAMAA"
     gene            3682..4446
                     /locus_tag="VGA59_06795"
     CDS             3682..4446
                     /locus_tag="VGA59_06795"
                     /inference="COORDINATES: protein motif:HMM:NF036878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9719425.1"
                     /translation="MSGLHVLVDFENVQPTFEEVAKLAPGVTEVWLFHGPHQVKLAEQ
                     FKAAHDDVTPVPISRTGKNALDFHLSFYLGYVAAKHPMGRLTVIANDKGYDPMIVHAK
                     ALGFTTQRIGFKVKKVAAAKKAPAAIKAVPVKKNPVAKKAAPSKKAAAKKAAPVKKAA
                     PAKKNITAKKSPVTKAASVQPEASSNPATAQVSRIAKGLAKMGDKRPEKLKSFLRHLE
                     SMLGKNTQPYAVQEAVSALEKAGLIQIIGDKVNYIE"
     gene            complement(4699..5937)
                     /locus_tag="VGA59_06800"
     CDS             complement(4699..5937)
                     /locus_tag="VGA59_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9719426.1"
                     /translation="MTYPFLNRLRVIESSAFIAAPLAGLTLAQFGADVIRVDMIGGGI
                     DYARMPRMPGGRSLYWTGLNKGKRSVAIDLRRPAGRELVQALATAPGPDAGVLLTNIG
                     TPWLAHDVLASRRADAISCTIQGNADGSTAVDYTVNCATGYPAVTGGGSRERPVNHAL
                     PAWDIACAYQAAFAVVAAVDHRRRTGAGAQLRLALSDMAFTTLSHLGVLAEAELLDQE
                     RPSIGNHLYGAFGRDFATADGQRVMIAAISAGQWKALVAACDAAPAVAELARATGLDF
                     ADEAQRFEARDALAELLEPWFATRSQAEVAAVLDAHKVCWGPYRTVRELLATDARVSM
                     ANPVFERLHTPGIGEHLAAGAAVRAPAMAREATPPAALLGSHTDEVLHEVLGLDSSAI
                     GRLHDAGVVAGPDADPSQRP"
     gene            complement(5949..>7012)
                     /locus_tag="VGA59_06805"
     CDS             complement(5949..>7012)
                     /locus_tag="VGA59_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9719427.1"
                     /translation="ARIEDVVFAQSYTNGEVPCTGRWVALQAGFPIEVAGMQLDRRCG
                     SGLQAVAVAAMMVQTGAADVVMAGGVESMSNVEYYTTGMRWGARAGSVKLHDRLERGR
                     ERSQPESRFGRISGMIETAENLARDFAISREQADAFALRSHQRAAAAWAAGKFAQEIV
                     PVSVAQKKGDPVAVDHDEGIRADLSLAQLAKLKPLMKDGIVTAGNACQQNDAAAACLV
                     VAEDKLAALGLTPIGYLHSWAAVGCEPSHMGIGPVPAVKKLFGRNGLSFDDMGLVELN
                     EAFACQALAVAKGWGWNDPERLNVNGSGISLGHPVGATGVRIMTSLLYELQRRGVRYG
                     LETMCIGGGQGLAAVFESA"
BASE COUNT         1286 a         2188 c         2344 g         1194 t
ORIGIN      
        1 gccgatgtgg ctttgcacaa gagccggtac ttcggctccg cctgggccaa ctacgaaagt
       61 gccaggcctg gcagcctgaa gctggtacct ccgggatacc gctggggcgc catcgaggcc
      121 gattatgtga agatgcggcc catgtttctg gatgagccag caccgttcgc cgaattgatg
      181 caccaggtcg ccggtgctga aaaagcgctc aacgcgctgt gacaccgatg acggccagcc
      241 actccaattt cgctttcctg caaaccgagt ggccagccct tcatgccgag gccttgcgag
      301 cggaaagctc gctggctacc gacccgcgcg ccgcctgctt ctatgcccgc cgcacactgg
      361 agctggcggt ggcctggctg ttccgcgccg accctgcgct caagatgccc tacaaggccg
      421 atctttcggc ctttttgttc gagcccagct tcaaggtgct ggtgggcccg gcgctgcacg
      481 ccaagatgga cgtcatccgc aagcagggca atagcgccgt gcacagcgcg cggccggtgc
      541 agccggcgga tgcactgggc gtgctgcgcg aactgttcca ggtggcgttc tggctggcgc
      601 ggcactacgg ccgcggtgca ggggcgcggc ccgatccggc cttgcagttc cgcgctgacc
      661 tattgcccaa agctgatgct gcggctgcag cgcaaacctt ggcggcgctg caaaagctgg
      721 cgcaggactt ggccgagcgc gacgcaacgg tcgccactgc ccagaaaaaa gccgcggcgc
      781 tggacgagga gctggcccgc ctgcgtgccg aagtggcggc agccaaggca gtcaacaatg
      841 cccaacccga cgcgcacgac tacaacgagg cgcagacgcg cgacctgtac atcgacctgt
      901 tgctcaaaga ggccggctgg gcgctcgatc aaacgcggga ccgcgagttt gaagtcgccg
      961 gcatgcccaa caaccagggc caggggtttg tcgattacgt gctttggggc gacgacggca
     1021 agcccctggc cgtggtcgag gccaagcgca cgcttaaaga cagccgcgtc ggccagcagc
     1081 aggccaagct gtatgccgat tgcctgcagg cgcagttcgg ccagcggccg ctgatctact
     1141 acaccaatgg ctacgagcac tggttctggg acgattcgcg gtacccgccg cggccggtgc
     1201 agggctttca caagaaggac gaactgcagt tgctgatgca gcggcgcggc agccgcaagc
     1261 cgctggctgc agcgcccatc gatggcgcga tcgtcgagcg acactatcag caccgcgcaa
     1321 tccggcgtat cgccgaaact ttcgagcagg acaagcagcg caaggcactg gtggtgatgg
     1381 ccacaggtgc cggcaagacg cgcatggtga tcgcgctggt ggacatgctg atgcgcggca
     1441 actgggccaa gcgcgtgttg ttcctggccg accgggtggc gctggtgaac caggcggtga
     1501 atgcgttcaa ggcccatctg cccgatgcag cgccggtgaa tctggtcacc gacaagtacg
     1561 gcgaaggccg tgtctacgtg tccacctacc ccacgatgat ggggttgatc aacgaaacca
     1621 acgaccagca gcgccgcttc ggtgtgggcc acttcgacct gatcgtcgtg gatgaggcgc
     1681 atcgatccat ctaccagaag taccgagcga tcttttcgta tttcgatgcc ttcctggtag
     1741 gtctcacggc cacgcccaag gacgagatcg accgcaacac gtacagcctg ttcgacctgg
     1801 aaaacggcgt gccgactgat gcttatgggc tggacgacgc catcgcagaa ggttacctgg
     1861 tgccgcctcg ggcagtgtcg gtgccgctga agtttcagcg cgagggcatc aagtactccg
     1921 atctgagcga ggaagatcgc gagcagtggg aggcgctgga ttgggacgac gaaggcggca
     1981 tcccggagga agtgaacgcc gacgcggtga acaagtggtt gttcaacaag gacaccgtgg
     2041 acaaagtgct ggaaacacta atgacacgcg gccagaaggt ggcgggcggc gaccggctgg
     2101 gcaagaccat tgtcttcgcc aagaacaacg accatgccga attcattgcc gagcgcttca
     2161 acgcgaacta tccgcactat gccggcctct tcgcacgggt gatcaccttc aagaccgagt
     2221 acgcgcagag cctgatcgac gatttctcgg tgaaggacaa gtcgccgcac atcgcggttt
     2281 cggtcgacat gctggacacc ggcatcgacg tgcccgaggt gctgaacctg gtgttcttca
     2341 agatcgtgcg gtccaagacc aagttctggc agatggtggg acgcggcact cggctgtgca
     2401 aggacttgtt tggcccgggg caggacaagc gcgacttctt gattttcgat ttctgccaga
     2461 acctcgagta cttcagccag gacctcccca cgacggaggg agccgtgggt cagcctttgg
     2521 gtcagcggct attcaatgcc aggttggagc tgattgccgc gctcgaccag cgatgcgctg
     2581 ccagtggccg ccaagccgcg gaggggtcgg cgcttggtgg ggtgcggctg accgaagaga
     2641 gcatccgcag cgagactgcc ggcatgctgc accgcatcgt cgccgggatg agtctggata
     2701 actttgtggt gcggccaaag cgcaaatggg ttcaggcctg ggcccacggt agcgcgtgga
     2761 atcgactcgg cgcagaccag cttcaggaaa tcagcgagca cttgtccggc ttgccgactg
     2821 ccgtgcgcga cgatgatgaa gaggccaagc gcttcgacct gttgatgctg cgcttgcagc
     2881 tatgcatgtt gcgggcggag ccaggcttcg agcgactgca gcaacaggtg cgcgagatcg
     2941 cggaccgtct atctgagctg ggcagcattc cggcggtgcg ggagcagatg ctgctgatcg
     3001 acgcggtggc tggcgaggag tggtggaaag atgtgaccat tcccatgctg gagcaggccc
     3061 ggcggcatct gcgcgtcttg gtgaagctgc tggataagtc ggggagaaaa gtcgtttaca
     3121 ccgatttcga agacgaactg ggcgagtcaa cggatgttga gttgcccgta ggcggcagtg
     3181 ccggcaattt cgagcgcttt cgcgtcaagg cgcgcgcttt cctgcgcgcg catgaggacc
     3241 atatcgccct gcacaagctg cgccgcaatc aggcgctgac cccatcggat ttggctgaac
     3301 tggagcgcat gcttgcggaa agcggcgtcg gtaccgcgca agatatcgag caggccaagc
     3361 aagaggcaca gggcctgggc ctgttcatcc gctcgctggt ggggctggac cgccaggcag
     3421 cgaccgaggc gctggctgtg ttcctggcag acaagaccct cagcggcaac cagatcgagt
     3481 tcgtcaacat gatggtgaac cacctgacgg aacacggcgt gatgaagccc gagctgctgt
     3541 acgaatcgcc gttcaccgcg tatgcgcctc aggggcccga caggttgttc tcatccgcgc
     3601 aggtggacga gcttttcaga gtgttggagc aggtcagggc gacagccatg gccgcttgac
     3661 gtcggggata aaggaaagcg aatgagtggt cttcatgtgc tggtggattt tgagaatgtt
     3721 cagcccactt tcgaggaggt agcgaagctt gctccggggg tgacggaggt ctggctgttc
     3781 catggcccgc accaggtcaa gttggccgag cagttcaagg ctgcgcatga tgacgtaact
     3841 ccggtgccta tttcgagaac gggcaagaac gcgctggatt ttcacctttc gttctatctt
     3901 ggttatgtcg cggcaaagca cccgatgggt cgattgaccg taatagccaa tgacaagggg
     3961 tatgacccca tgatcgttca cgcgaaggcg ttgggcttta cgacccagcg cattggcttc
     4021 aaggtcaaaa aggtagccgc tgcgaagaaa gcacctgcgg caataaaagc tgtgccggtc
     4081 aagaaaaacc ccgtggcaaa gaaagccgcg ccctcaaaga aagccgcagc caagaaggcc
     4141 gcgcctgtca agaaggctgc gccggcgaaa aagaacatta ccgccaaaaa gtcgccggtc
     4201 accaaggccg cgtcggtcca gccagaggcc agctcaaatc ctgccacagc gcaggttagc
     4261 cgcatcgcga agggattggc caagatgggc gacaagcgtc ccgagaagct caagtctttt
     4321 ctgcggcatc ttgaatcgat gttgggcaag aacacccagc catatgccgt gcaggaggca
     4381 gttagtgcac tggagaaggc aggcttgatc cagatcatcg gagataaggt caactatatc
     4441 gagtgatatg gcccttggct tttgcccgac tgatcgctta cgctttcttc cggcctgtgg
     4501 cgcgtggacg cgcctgcatc atggagaact ccctggccac cgtcttgtga aacgcgtcga
     4561 tgaatcttgg gttgatccca actccagccc ggaggccctc gccagccagc gaatcgccct
     4621 tggtgtagta catgtgccag gggcggctga cctgagtccg ccggccggat ccgggcgctc
     4681 attcacgcat ggtgggcgtc agggacgctg gctcggatcg gcatcaggcc cggccaccac
     4741 gcccgcgtcg tgcaggcggc cgatggcact gctgtccagc cccagcactt catgaagtac
     4801 ttcgtcggtg tggctgccca gcagcgccgc gggcggcgtg gcctcgcgcg ccatcgcggg
     4861 ggcacgcacg gcggcgccgg ctgcgaggtg ctcgccgatg cccggtgtgt gcaggcgttc
     4921 gaacaccggg ttggccatgc tgacgcgcgc gtcggtggcc agcagctccc gcactgtgcg
     4981 gtaggggccc cagcagacct tgtgggcatc gagcacggcg gccacctcgg cttgcgaccg
     5041 ggtcgcgaac cagggctcca gcagctcggc cagcgcgtcg cgtgcttcga agcgctgggc
     5101 ttcgtcggcg aagtcgagcc cggtggcccg cgccagttca gccacggccg gtgccgcgtc
     5161 gcaagccgcg accagcgctt tccactggcc cgcggagatc gccgcgatca tcacgcgctg
     5221 gccgtcggcg gtggcgaagt cgcggccaaa ggcgccgtag agatggttgc cgatggaggg
     5281 gcgttcctgg tccagcagct cggcctcggc cagcacgccc aggtgcgaaa gcgtggtgaa
     5341 cgccatgtcc gagagcgcca gccgcagctg cgcgcccgcg ccggtgcggc gccggtggtc
     5401 cacggccgcc accaccgcga acgcggcctg gtaggcacag gcgatgtccc aggccggcag
     5461 cgcgtggttg accggccgct cgcgcgagcc gccgcccgtg acggcgggat agcccgtggc
     5521 gcagttgacg gtgtagtcga ccgcggtcga cccatcggcg ttaccctgaa tcgtgcaact
     5581 gatggcatcg gcgcggcgcg atgccagcac gtcatgcgcg agccagggcg ttccgatgtt
     5641 ggtcagcagc acgccggcat cgggccccgg cgcggttgcc agcgcctgta ccagctcgcg
     5701 gccagcgggc cgtcgcaggt cgatcgcaac cgagcgtttg cccttgttga ggccggtcca
     5761 gtagaggctg cgcccgccgg gcatgcgtgg catgcgcgcg tagtcgatgc cgccgccgat
     5821 catgtccacg cgaatcacgt cggcaccgaa ctgcgcgagc gtcagacccg ccagcggcgc
     5881 ggcaatgaaa gccgagctct cgatcacgcg caggcggttg aggaagggat aggtcatgtc
     5941 cgggctctct aggcgctctc gaatactgcc gcgaggccct ggccgccgcc gatgcacatg
     6001 gtctccaaac catagcgtac gccgcggcgc tgcagctcat acaacagcga cgtcatgatg
     6061 cgcaccccgg tggcgccgac cgggtggccg agggagatac ccgagccgtt gacgttcagg
     6121 cgctccgggt cgttccagcc ccagcccttg gcgacggcca gggcctggca ggcgaaggcc
     6181 tcgttgagtt cgaccaggcc catgtcgtcg aacgagagcc cgttcctgcc gaagagtttc
     6241 ttcacagccg gcaccgggcc gatgcccatg tgcgagggct cgcagcccac cgcggcccag
     6301 ctgtgcaggt aaccgatggg cgtcaggccc agcgcggcca gcttgtcttc ggccacgacc
     6361 aggcaggccg cggccgcgtc gttctgctgg caggcgttgc cggcggtcac gatgccgtcc
     6421 ttcatgagtg gcttcagttt cgcgagctgc gccaggctca ggtctgcgcg gatgccttcg
     6481 tcgtgatcga ccgcgaccgg gtcgcccttc ttctgcgcga cactgaccgg cacgatctcc
     6541 tgggcaaatt tgcccgcggc ccaggccgca gccgcacgct ggtggctgcg caatgcgaaa
     6601 gcgtcggcct gctcgcgcga gatggcgaag tcgcgcgcca ggttctccgc ggtctcgatc
     6661 attcccgaga tgcggccgaa gcgcgactcg ggttgcgagc gctcgcgccc gcgctccagc
     6721 cggtcatgca gcttgacgct gccggcgcgt gcaccccagc gcatgccggt cgtgtagtac
     6781 tcgacgttgc tcatgctctc gacaccgccg gccatcacca cgtcggccgc gccggtttgc
     6841 accatcatcg cggccaccgc gacggcttgc aggccgctgc cgcaacggcg gtcgagctgc
     6901 atgcccgcga cttcgatcgg aaagcccgcc tgcagcgcga cccagcggcc ggtgcaaggc
     6961 acttcgccgt tggtgtagga ctgggcgaag accacgtctt caatgcgcgc cg
//
LOCUS       DASWIY010000180         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1586951, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000180 DASWIY010000000
VERSION     DASWIY010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586951"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VGA59_06810"
     CDS             <1..891
                     /locus_tag="VGA59_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9719428.1"
                     /translation="TMFTQSNQYRVVLEVKPQFQDDLQDLQHVYLRSASRGQVPLSAV
                     TRVETSTAPLAINHQGQFPVVTISFNLAPGISLGEAVQVIDAAKQELGLPASMVSGFQ
                     GTAKAFQASLANQPLLILAALVTVYIVLGVLYESYIHPATILSTLPSAGLGALLALMV
                     CRIDLNVIALIGIILLIGIVEKNGIMIVDFALDAERHDGKEPLDAIFQACLLRFRPIL
                     MTTMAALLAGVPLAFGGGTGAELRRPLGITMIGGLMVSQVLTLFTTPVIYLAFDRLAR
                     RVGKRRGTPVVRQAESVEVV"
     gene            888..>1684
                     /locus_tag="VGA59_06815"
     CDS             888..>1684
                     /locus_tag="VGA59_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9719429.1"
                     /translation="MNISALFIHRPVGTTLLTVALALMGAVAFQFLPVSPLPQVEFPT
                     IEVRAGLPGASPETMASAVTTPLERQLGRIAGLNEMTSVSTLGLARITLQFDLSKNIN
                     AAARDVQAAINAARGQLPANLPETPRFRKTNPADVSIIALALTSDVVDRGRMYDVASS
                     ILEQKLSQVEGVGLVRIGGGALPAVRVEVNPTILNSYGLSLEDVRAVLDSANANRPKG
                     ELADGSRAWALSTTDQLLKAGEYQPLIISYKDGRAVRLSDVATVRDSV"
BASE COUNT          289 a          557 c          512 g          326 t
ORIGIN      
        1 accatgttca cccaatcgaa ccaataccgg gtcgtgctgg aagtcaagcc gcagttccag
       61 gacgatttac aagatttgca gcatgtgtac ctgcgctcgg cttcacgtgg acaagtaccg
      121 ctcagtgccg tcacccgtgt cgagacgtcc acggcgccgc tggccatcaa ccaccagggg
      181 cagtttccgg tggtcaccat ttcgttcaat ctcgcgcccg gcatctccct gggggaggcg
      241 gtgcaggtca tcgacgcagc caagcaggag ctgggccttc ctgccagcat ggtgtcgggc
      301 tttcagggca cggcaaaggc cttccaggcc tcgctggcga accaaccgct gctcattctg
      361 gcggccctgg tgacggtgta tatcgtcctg ggcgtcctgt acgaaagtta cattcacccg
      421 gcaacgattc tctccacgct gccctcggct ggcctgggcg cgctcctggc gcttatggtt
      481 tgccgcatcg atctaaatgt catcgcgctc atcggcatca tcctgctgat cggcatcgtg
      541 gaaaagaacg gcatcatgat cgtcgacttc gccctcgatg ccgagcgcca tgatggcaag
      601 gaaccgctcg atgccatctt ccaggcgtgt ttgctgcggt ttcgtcccat cctcatgacc
      661 accatggccg ccttactggc cggtgtgccc ctcgcctttg gcggtggcac cggtgccgag
      721 ctgcgccgcc cgctgggcat cacgatgatc ggcgggctca tggtcagcca ggtgctgacg
      781 ctgtttacga caccggtgat ttacctcgcc ttcgatcgcc tggcgaggcg tgtgggcaag
      841 cgccgtggca cgcccgtcgt gcggcaagcg gaatccgtgg aggtcgtatg aatatctcgg
      901 ccctgttcat ccaccgacca gttgggacca cgctgctgac cgtggcgctg gcgctcatgg
      961 gtgccgtcgc cttccagttt ttgccggtct cgccgctgcc tcaggtggag tttcccacca
     1021 ttgaggtcag ggccggactg cccggcgcca gtccggaaac catggcctcc gccgtgacca
     1081 caccgctcga gcgccagctt gggcgcatcg ccgggctgaa cgaaatgacc tccgtcagca
     1141 cgctcggcct agcgcgcatc accctgcagt tcgacctcag caagaacatt aacgccgcgg
     1201 cgcgcgacgt gcaggcagcg atcaacgccg cacgcggaca gctgccggcc aacctgccgg
     1261 agacgcccag gttccgcaag accaatccgg cagacgtctc catcatcgct ctggcgctca
     1321 cctccgacgt ggtggaccgg ggccgcatgt acgacgtcgc ctcctccatt ctggagcaga
     1381 agctctctca agtcgaaggc gtcggactgg tcaggatcgg agggggagcg ttgccggcgg
     1441 tgcgggtgga agtcaatccc accatcttga atagttatgg cctgagcctc gaagatgtgc
     1501 gtgcggtgct ggacagcgcc aacgccaatc gccccaaggg cgaactggct gacggcagcc
     1561 gcgcctgggc tctcagcacg accgaccaac tgctgaaggc cggggaatat cagccgctga
     1621 tcatttccta taaggatggc cgggcagtgc gcctctccga tgtcgccacc gtccgggatt
     1681 cggt
//
LOCUS       DASWIY010000181         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2334865, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000181 DASWIY010000000
VERSION     DASWIY010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2334865"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..486)
                     /locus_tag="VGA59_06820"
     CDS             complement(160..486)
                     /locus_tag="VGA59_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX9719430.1"
                     /translation="MTTPPANRRLHPVTLLQAAQDSPTLARLAQLARESGERLEAVEL
                     LIPASLRSAVRPGPIDGPAWCLLVDSNAAAAKLRQVIPALLNRLRDRGWQVTSIRLKV
                     QIVPGK"
     gene            499..1476
                     /locus_tag="VGA59_06825"
     CDS             499..1476
                     /locus_tag="VGA59_06825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9719431.1"
                     /translation="MHFIITDAWLAKSRAVHLSGTKLVLVFAALSLALMLMAAGLYHW
                     VFLKGAREGWPVVGTLVKLVVKDEFEQRDRFLRENLDVMARKLGEMQAKMVQLEALGE
                     RVSGLAGVNPAEIKGAPARGGALVSGRPLSMEELQATLADLDELSAQRTDLLTVMESR
                     LFDQKIKKMMVPTRQPVQDGHVGSPFGWRIDPFTGRSALHTGLDFQADAGTPILAAAG
                     GVVVAQEAHPAYGNMLEIDHGNNLITRYAHASKLWVKKGDLVKRGQKIAAVGSTGRST
                     GPHLHFEVMVQGVPQDPQKFLAAGRGLPALHLAKAARLGGAPMQESARR"
     gene            1598..>2407
                     /locus_tag="VGA59_06830"
     CDS             1598..>2407
                     /locus_tag="VGA59_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9719432.1"
                     /translation="MAKNFLTQIFGSRNDRLLKQYRKTVDRINALEPQFEKLSDDELR
                     GKTQEFKDRLAKGEELDDLLVEAFAVVREGSKRVMKMRHFDVQLMGGIALHNSKIGEM
                     GTGEGKTLVATLPVYLNALTGKGVHVVTVNDYLANRDAQWMGKLYSFLGMTVGINLPN
                     MQREEKQAAYRADITYGTNNEYGFDYLRDNMVYEVADRVQRGLNYAIVDEVDSILIDE
                     ARTPLIISGQAEDHTDLYIAINRVVPLLSQQEGEADPRTGEGVIKPGVFTVD"
BASE COUNT          450 a          766 c          797 g          394 t
ORIGIN      
        1 ctcctcaatg gcgaaatagc catccagttg gtcggcgtcg ccactcccga tggatctatt
       61 gccatggcat gagccggcgc cttgcattcg cgcagcggtt ttccttgata cggatcaact
      121 gcgccctggg ttgttttagg ggtaagcccg gggggcttct cacttgcccg gaacgatttg
      181 aaccttcagc cgaattgagg taacctgcca gccccggtcg cgtaaacggt tgagcagagc
      241 agggatgacc tgcctcaatt tcgcggccgc ggcgttgctg tccaccagca ggcaccaggc
      301 gggtccatcg atcggaccgg gcctgacggc ggaccgcagc gaagcgggga tcaacaattc
      361 aaccgcttcg agccgttcgc cggactcccg tgcgagctgc gcgagcctcg ccagggtggg
      421 cgagtcctgc gcagcctgca gcaaagtgac gggatgcagg cgccggttcg cgggtggtgt
      481 cgtcatgagg ggatagcggt gcatttcatt atcacggatg cgtggcttgc gaaaagccgc
      541 gccgtgcacc tcagcggtac caaactggtg ctggtgttcg ccgccttgtc cctggccctg
      601 atgctgatgg ccgccgggct ctaccactgg gtgttcctca agggcgcgcg cgagggctgg
      661 ccggtcgtcg gcacgctggt gaagctggtc gtgaaggacg aattcgagca gcgggatcgc
      721 ttcctgcgcg aaaacctcga cgtcatggcc cgcaagctcg gggaaatgca ggccaagatg
      781 gtccagctcg aggccctcgg tgagcgcgtc tcgggcctgg ccggcgtgaa tccggcggag
      841 atcaagggcg cacccgcgcg cggcggcgcg ctggtgtcgg gccgtccgct gtccatggag
      901 gaattgcagg ccacgctggc ggacctggac gagctgagcg cccagcgcac cgacctgctg
      961 acggtgatgg agtcgcgcct tttcgaccag aagatcaaga agatgatggt gccgacccgg
     1021 caacccgtgc aggacggcca cgtgggctcg ccgttcggct ggcgcatcga cccgttcacg
     1081 ggacgctccg cgctgcacac cgggctggat ttccaggcgg acgcgggcac gcccatcctg
     1141 gcggccgcgg gcggcgtggt cgtggcgcag gaagctcacc ctgcctacgg caacatgctc
     1201 gagatcgacc acgggaacaa tctcatcacc cgttacgccc atgcatccaa gctgtgggtg
     1261 aagaagggcg acctggtcaa gcgcggccag aagatcgccg cggtcggctc gaccggccgc
     1321 tccaccgggc cgcacctgca cttcgaggtc atggttcagg gcgtgcctca ggatccgcag
     1381 aaattcctgg cggcggggcg gggtttgccg gccttgcatc tggcaaaggc cgcgcgcctc
     1441 ggcggcgcgc cgatgcaaga gtcggcccgc aggtaaaatc ctccgatgtg ctgctgtcgg
     1501 cggcttgctt tggcacgcgg cagccccatt tcatcctgac aacacaagga tcgcgcagcc
     1561 ctgccggcct cacgcccggc agggcggcaa cgcattgatg gccaagaact tcctgacaca
     1621 aatattcggt tcccgcaacg accgcctcct gaagcaatac cgcaagacgg tcgatcggat
     1681 caatgcgctg gagccgcagt tcgaaaagct cagcgacgat gaactgcgcg gcaagacgca
     1741 ggagttcaag gaccggctgg ccaagggcga ggagctcgat gacctcctgg tcgaggcttt
     1801 cgccgtcgtg cgcgagggct ccaagcgcgt catgaagatg cgccacttcg acgtccagct
     1861 catgggcggc attgcgctgc acaacagcaa gatcggcgag atgggcaccg gcgaaggcaa
     1921 gacgctggtg gcgacactcc cggtttacct caatgccctc acaggcaagg gcgtccatgt
     1981 cgtcaccgtc aacgattacc tggcgaaccg ggacgcgcag tggatgggca agctctacag
     2041 ctttctcggc atgacggtcg ggatcaacct gccgaacatg cagcgcgagg agaagcaggc
     2101 agcctaccgc gccgacatca cctacggcac caacaacgag tacggtttcg actacctgcg
     2161 cgacaacatg gtgtacgaag tggcggaccg ggtccagcgc ggcctgaact acgccatcgt
     2221 cgacgaagtg gactccatcc tgatcgacga agcgcgcacc ccgctgatca tcagcgggca
     2281 ggccgaggat cacacggacc tgtacatcgc gatcaaccgc gtcgtgcccc tgctgtccca
     2341 gcaagagggc gaggcggacc cgcgcacggg cgagggcgtc atcaagcccg gcgtcttcac
     2401 agtggac
//
LOCUS       DASWIY010000182         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_841218, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000182 DASWIY010000000
VERSION     DASWIY010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841218"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(455..1896)
                     /locus_tag="VGA59_06835"
     CDS             complement(join(455..1507,1510..1896))
                     /locus_tag="VGA59_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611236.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX9719433.1"
                     /translation="MPPGSDSEVVPRARRRTFTNADKRRILQAADLCTLPGEVGALMR
                     REGVYSSSLSTWRRQREAADLAALAPQKRGPKVDAGARIDALQIAQLTRERDKLRVEL
                     DKAQLVIEVQKKVTALLDLLAATDKRGHLMAAALELTPTLGAGAACRAMGLWRGVPAR
                     QQARVHRRTAMGPPRPRAPRPCPPLALDASERQAVLDTLNSERFADTAPAAVHATLLD
                     EGTYLGSVRTMYRLLAANGASRERRNQLIHPAYTKPELLATAPNQVWSWDITKLKGPA
                     KWTCFHLYVILDIFSRFVVGWLIAPRECSELAQQLIADTVSRHNVQPGMLTLHADRGA
                     AMRSKPVASLLVDLDVAKSHSRPHVSDDNPYSESQFKTMKYRPAFPQRFGCIEDARAH
                     CQAFFAWYNAEHCHSGIAHMTPHSVHYGLADSMRVIRQATLDGAFLANPSRFKNKRPQ
                     LPPMPTAAWINPPPEEKTPTSEPVARTLN"
BASE COUNT          357 a          678 c          824 g          453 t
ORIGIN      
        1 ttcacactcg actcggcgac gctgttgccg ctgcggcctt gttccttgcg aacgtggact
       61 ggtacgggcg ccagcacgcc cttcgcgaag tcgccgagca attgcatccc ggtagcaaga
      121 atttctcggc gttggcttcg caggcgaact ttgactgcgg ccgattctca agcatgctca
      181 agatgagact cgatcatgcc tcacgcctat cctgagctta cgggcgcgtt ggcccagatg
      241 ctcgacaaga tcgatacgta cctgcgcgac tccgggtacg aagggccgcc tgtgaggatg
      301 tacttggcgg gcggattagc ggtgaactac tactgcggga gccgatacac cgaggacgtc
      361 gacgcttcgt tctcccgccg cgttgtcgtg cgttccgagg atctcgtcat cgactacccg
      421 gaacgtgtca atgattttgc gacacccggg gacgttaatt tagtgtgcgg gcgacgggct
      481 cactggtggg agtcttctcc tcaggtgggg ggttgatcca cgcggcggtc ggcatcggcg
      541 ggagctgggg tcgcttgttt ttgaaccggc tggggttagc caggaatgcg ccatcaaggg
      601 tggcctggcg gatgacacgc atgctgtcgg ccaagccgta gtggacgctg tgcggggtca
      661 tgtgggcgat gccgctatgg cagtgctcgg cgttgtacca ggcaaagaag gcttggcagt
      721 gggctcgcgc gtcctcgatg cagccgaagc gctgagggaa tgcgggccgg tacttcatgg
      781 tcttgaactg cgactcggag tacgggttgt cgtccgagac gtgaggtcgg ctgtggctct
      841 tggcgacatc gaggtcgacc agcaggctgg cgaccggttt ggagcgcatg gccgcacccc
      901 ggtcggcgtg cagggtcagc atgccgggct ggacgttgtg ccgcgacacc gtgtcggcaa
      961 tgagctgctg ggcgagctcg gagcactcgc gcggggcgat gagccagccc acgacgaagc
     1021 ggctgaagat gtccaggatc acgtagagat ggaagcaggt ccacttcgcg gggcccttga
     1081 gcttggtgat atcccaggac cagacttggt tgggcgccgt ggccagcagt tcgggcttgg
     1141 tataggccgg gtggatgagc tggttgcgcc gctcacggct ggcgccgttg gccgccagta
     1201 gccggtacat ggtgcgtacc gagccgaggt aggtgccctc gtcgagcagc gtggcgtgca
     1261 cggccgctgg cgcggtgtcg gcgaagcgtt cgctgttcag ggtgtcgagc accgcctgcc
     1321 gttcgctggc atccagggcc aggggcgggc aagggcgcgg tgcccgcggc cggggcggcc
     1381 ccattgccgt gcggcggtgc acgcgggcct gctggcgtgc tggcacgcca cgccacagac
     1441 ccatggcgcg acacgccgcg ccggcgccga gcgtgggggt gagctccaac gcggcggcca
     1501 tcaagtgttc ccgcgcttgt cggtggccgc tagcagatcg agcagcgcag taactttttt
     1561 ttggacctcg atcaccagct gcgccttgtc gagttcgacg cgcagcttgt cgcgctcgcg
     1621 ggtgagctgg gcaatctgca gtgcatcaat gcgcgcgcca gcgtccacct tggggccgcg
     1681 cttttgcggc gccagtgccg ccaggtccgc tgcctcgcgt tggcgccgcc aggtgctcag
     1741 ggacgatgag tacacgccct cgcggcgcat cagcgcgccg acttctccgg gcagggtgca
     1801 caggtcggcg gcctgcagaa tgcggcgctt gtcggcgttg gtgaaggtgc ggcgacgcgc
     1861 gcgcggcacc acctcggagt cgctgccggg cgggatcggc gacggcgccg atccgggcgc
     1921 atcttcagtc gccctacggg ctccttgcga tgcgcccgga tcagaagaaa ccttcactgc
     1981 gtgcatgggg accttcaatt gctggttgct cttcatacct acctctttgc tcactgatct
     2041 ctcgggggta ggtgtcgcag ctcatattgg cacagggggg gccctgaaaa atttggctat
     2101 ccggattccg cgattgatat gcagatggtt ttgtgcgtcg caggacgtcg ctagtggact
     2161 gctaaattcc aaaggaagtg ggatgcctgc catcgaccca ttgcggtccc tcgcgctgct
     2221 gggccgagag actgtttagg cggagaaagc cgactcattc agctggccgc tcgacggtcg
     2281 gtagatagtt cgggggctgc cggtcgaaca gc
//
LOCUS       DASWIY010000183         3789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_841696, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000183 DASWIY010000000
VERSION     DASWIY010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841696"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VGA59_06840"
     CDS             <1..297
                     /locus_tag="VGA59_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX9719434.1"
                     /translation="VGSSLQANVELTVPMATHGELWSALASLGDDLKFVFITSVMRVV
                     AGDTMAVKVTPSEAVKCERCWHWRNDVGQDSDHPTICGRCTSNLNGPGEDRKFA"
     gene            297..785
                     /gene="lspA"
                     /locus_tag="VGA59_06845"
     CDS             297..785
                     /gene="lspA"
                     /locus_tag="VGA59_06845"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX9719435.1"
                     /translation="MFRAKQSSWLHWLGLALAIIIADQLTKVLIMGNYRLGDSTYVAS
                     FFNIVRAHNTGAAFSFLAGASGWQRWFFTAFGIAAAIFITWMLRSHPGQKLFSFSLAC
                     ILGGAIGNVVDRLLHGYVVDFIQVHWRGWYFPAFNVADSAITMGAALLILDELLRVRR
                     AR"
     gene            893..2572
                     /locus_tag="VGA59_06850"
     CDS             893..2572
                     /locus_tag="VGA59_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEX9719436.1"
                     /translation="MKHLLNLLAAIALLVWGTHLVRTGILRVFGANLRNVLAHSVANR
                     WTAAVSGLGVTALVQSSTATALIVSSFVGQGLVTLPMALAVMLGADIGTSLMTVVFSF
                     DLSWLSPLFIFVGVVLYMSRESHPVGRFGRIMIGLGLMLLALRLVAASTEIMTHSATV
                     KALLGSISTDLLLEITVGAFLSVVAYSSLAIVLLIATMAASGLIPLEPALGLVLGANL
                     GNGLVGVLTTVRSAVEVRQVPLGNFLFKALGVLIAAPLIGLWLRHVRPYVHDPATGVV
                     LFHLVFNFMVAALFIGLTRPVARWVDHWLPKPDRSLVAGRPHHLDPSALETPSLAISC
                     AAREALHQADVVETMITGMLPVIKNNDLKLAQDLRKLDDHVDELYSAIKYYLTKISRE
                     ALGEEESRRWTDIISFTINMEQIGDIVERILIDIEDKKIRKGRSFSDAGMAEICELHA
                     RLIDNLRLGMSVFLNGSVRDAQKLLEEKARFRDLERAYASTHLTRLSDNSVQSIETSS
                     LHIDLISDLKRINSHICSIAYPILDSAGALAPSRLRQAQLAEGPASRAQRL"
     gene            complement(2848..3234)
                     /locus_tag="VGA59_06855"
     CDS             complement(2848..3234)
                     /locus_tag="VGA59_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719437.1"
                     /translation="MKFRAYIVEDSAVIRENLIETLEELAHVEPVGSAETEYEGKKWL
                     ARNDSYWDLAIVDLFLKEGSGLNILEACRGRRPSQKIVVLSNHATNDVRWRCEQLGAD
                     AVFDKSTEIDELVDYCVSEQAKVKEE"
     gene            complement(3657..>3789)
                     /locus_tag="VGA59_06860"
     CDS             complement(3657..>3789)
                     /locus_tag="VGA59_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9719438.1"
                     /translation="LFEAVTSGKVKIRIDQRYPLEQVQQAHRDLEARKTTGSTILTL"
BASE COUNT          611 a         1247 c         1212 g          719 t
ORIGIN      
        1 gtcggctcgt cgctgcaggc caacgtggaa ctcactgttc ccatggcgac ccacggcgaa
       61 ctgtggtccg cgctggccag cctcggggac gacctgaaat tcgtcttcat cacctcggtc
      121 atgcgggtgg tggcaggcga cacgatggcc gtgaaggtca ccccgtccga ggcggtgaag
      181 tgcgagcgct gctggcactg gcgcaacgac gtcggccagg attccgacca tcccacgatc
      241 tgtgggcgct gcaccagcaa cctgaacggt cccggcgaag acaggaagtt cgcctgatgt
      301 ttcgcgcgaa acagagcagc tggctgcact ggctgggcct ggccctggcc atcatcatcg
      361 ccgaccagtt gaccaaagtg ctgatcatgg gcaattaccg gctgggcgac tccacttacg
      421 tcgccagctt cttcaacatc gtgcgggccc acaacaccgg cgctgcgttc tccttcctgg
      481 cgggggcctc gggctggcag cgctggttct ttaccgcctt cgggatcgcg gcggccatct
      541 tcatcacctg gatgctgcgc tctcatccgg gacaaaagct gttctccttt tccctggcct
      601 gcatcctggg cggggccatc ggcaacgtcg tggaccggct gcttcacggc tatgtcgtgg
      661 acttcatcca ggtgcattgg cgcggctggt acttcccggc cttcaacgtg gccgacagcg
      721 cgatcaccat gggcgccgca ttgcttatcc tggacgaact gctgcgggtc aggcgcgcca
      781 ggtaaccgcc ggacggcggt ccggcggcgc tcgacgtgcg cacgctaacg gtccaggccg
      841 ccggccttca cgatgctgcg ccgcaccaaa gcgtatagtt tgattcaacg caatgaagca
      901 tctgttgaat cttctggcag ccatcgcgct gctggtgtgg ggaacccatc tcgttcgtac
      961 cggcatcctg agggtgttcg gcgccaatct gcgcaacgtg ctggcccata gcgtcgccaa
     1021 tcgctggacg gcggccgtct caggcctggg cgtcacggcc ctggttcagt cgagcaccgc
     1081 caccgcgctc atcgtctcct cgttcgtggg ccaggggctg gtcacgctgc cgatggcgct
     1141 ggcggtgatg ctgggcgcgg acataggcac cagcctgatg acggtggtgt tttcgttcga
     1201 cctctcatgg ctgtcgccgc tgttcatctt cgtgggcgtg gtgctgtata tgtcgcgcga
     1261 gtcccacccc gtgggccgct tcggccgcat catgatcggg ctcgggctga tgctcctcgc
     1321 gctgcgcctg gtcgcggcgt cgaccgaaat catgacgcat tcggccacgg tgaaggcgct
     1381 gctgggttcg atctccaccg acctgctgct ggaaatcacg gtgggcgcct tcctctcggt
     1441 cgtggcctat tccagcctgg ccatcgtgct gctgatcgcc acgatggccg cgtcgggcct
     1501 gatcccgctg gagcccgcgc tcggcctggt gctgggcgcc aacctgggca acggcctggt
     1561 cggcgtgttg acgacagtcc gatcggccgt ggaggtgcgc caggtcccgc tgggcaactt
     1621 cctgttcaag gccctgggcg tgctcattgc cgcgcccctc atcggcctgt ggctgcgcca
     1681 tgtgcgcccc tacgtgcacg accccgccac cggcgtcgtg ctcttccacc tggtcttcaa
     1741 cttcatggtg gcggcgctgt tcatcgggct gacgcggccg gtggcgcgct gggtcgatca
     1801 ctggctgccc aagccggacc gcagcctggt ggcagggcgg ccgcaccacc tcgacccgtc
     1861 ggcgctggag acgccgtcgc tggcgatctc ctgcgcggcg cgcgaggcgc tgcaccaggc
     1921 cgacgtggtc gagacgatga tcacggggat gctgccggtg atcaagaaca acgacctcaa
     1981 gctcgcgcag gatttgcgca agctggacga ccatgtcgac gagctgtatt ccgcgatcaa
     2041 gtactacctg accaagattt cgcgcgaggc gctgggcgaa gaggaaagcc ggcgctggac
     2101 cgacatcatc agcttcacca tcaacatgga gcagatcggc gacatcgtcg agcggatact
     2161 gatcgacatc gaggacaaga agatcaggaa gggccgcagc ttttcggatg cgggcatggc
     2221 cgaaatctgc gagctgcatg cgcggctgat cgacaacctg cgactgggca tgagcgtctt
     2281 cctcaacggc tcggtgcgcg acgcgcagaa gctgctggag gagaaggcgc gctttcgcga
     2341 cctggagcgc gcttatgcgt cgacgcacct gacgcgcctg agcgacaact cggtgcagag
     2401 catcgaaacc agttcgctgc acatcgacct gatcagcgac ttgaagcgca tcaactcgca
     2461 catctgctcg atcgcttacc cgatcctcga ttcggccggc gcgctcgcgc ccagccggct
     2521 gcgccaggcg cagctcgcgg aaggccccgc cagccgggcg cagagactct gaccgggccc
     2581 gatcgggcat ccacgacttc ctggattgcg ggtcaggccc gcaatgacaa atcacctgtc
     2641 atgccggact cgatccggca tccatgactt tgtgccttgt ggattgtggg gccgggccgg
     2701 agcgagatgc ctcggcacgt gcatagcgca ggcccggcat tgggctcgcc gcactggaca
     2761 gctgcgcgct ccggcgttcc gcgagttccg cccttttcaa tgacagctat ttccagggca
     2821 gtcccgatcc gcggccgcaa cgggcagcta ttcttctttc accttggcct gctcactgac
     2881 gcagtagtcc acaagctcat cgatctcggt ggacttgtcg aaaaccgcgt cggcgcccaa
     2941 ttgttcgcag cgccagcgca cgtcgttggt ggcgtggttg ctcagcacca ctatcttctg
     3001 cgagggacgg cggccgcggc aggcttcgag gatgttcagg ccgctgcctt ccttcaggaa
     3061 caggtcgacg attgcaaggt cccagtacga atcgttccgg gccagccact tcttgccttc
     3121 gtattcagtc tcggccgagc ccacgggctc gacatgcgcc agttcctcca gtgtctcgat
     3181 gaggttctca cggatcaccg cgctgtcctc aacgatgtaa gcacggaact tcatgacttc
     3241 tccattggag agccgtgccc ggcgaaggcc ggcagcgcgg ctctcgctct ctgtggaaac
     3301 caatgtaccg cgctacgatc gagctggatg caagcaccaa ggccgagcag atgtaggaga
     3361 gctcgcagga cccacggttc gtcgcctgtc ggatggcgac tacaagcccg cttgcgacag
     3421 catgagcgcc gcttcgatcg tgaattcatc acgttccatg cgcgccagga cctcgccgcc
     3481 gtccatgatg tttcaccgac caggccggcg ggcatggcgg cctggcggaa gaaccccggc
     3541 tccaccgagc cgatgcgggc gtttgccgct ccacagcggc acacgcggat gcaacggcgg
     3601 ccggtcggcg cacgcatgga gcgcggccag ccattcggcc gcgggaaggg gccggctcac
     3661 agggtcagga tggtcgaacc cgtggtcttg cgcgcttcca ggtcgcggtg agcctgctgc
     3721 acctgttcca gcgggtagcg ctggtcgatc ctgatcttga ccttgccgct ggtcacggct
     3781 tcgaacagg
//
LOCUS       DASWIY010000184         7350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_100227, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000184 DASWIY010000000
VERSION     DASWIY010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100227"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VGA59_06865"
     CDS             <1..733
                     /locus_tag="VGA59_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEX9719439.1"
                     /translation="IEVFDEPEVAAARHRAGLRRLFALQIRDALKYLEKNVPDLQKMA
                     VAYMPLGTQEELREQIIEVALDRAFLQPPLPSNEADFKRRIDEGRARLTLIANEVARL
                     GAAILAEYATAVRKIRDTKNQPDATGDATGQLQRLMPKRFLAVTPWTQLQHFPRYLKA
                     ITLRLDKLRSDPARDAARLAELKPQEQRYWRLVAERKGAVDERMQEYRWLLEELRVSF
                     FAQELRTPQPVSVKRLDKLWSQLNS"
     gene            complement(759..917)
                     /locus_tag="VGA59_06870"
     CDS             complement(759..917)
                     /locus_tag="VGA59_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEX9719440.1"
                     /translation="MSISLILLILLILLLVGALPSWGHSRNWGYGPSGGLGLVVLILL
                     VLLLMGKI"
     gene            1181..2188
                     /locus_tag="VGA59_06875"
     CDS             1181..2188
                     /locus_tag="VGA59_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEX9719441.1"
                     /translation="MTLASLALQFAVCALLIARAGFVLSSSADRLAGTYGWGRGWVGL
                     TLLATVTSLPELASGISAVAFVDAPNLAVGNALGACVVNLAFLVVVDVLQRNQPMYRD
                     ASATHLLSAGFGVVMLGFIAMSLLTGTRAPAVLHVGIYSPLLLGLYLLALRGVHAHER
                     TAMAGCAAAAHPASAPSDTRREWRRFGLAALIVLAAGGWLPEVADALAQTLGLSRSFV
                     GTVFMAVVTTLPEIAVTVSALRLGALDMAIGNLLGSNLFNVLILAVDDTFYTKGPLLA
                     DAAPVHVGTAVTALVMTGLVIIGLVMRPQGRVLRVLSWVSVGLVAAYVINASLAYLGG
                     A"
     gene            2230..5061
                     /locus_tag="VGA59_06880"
     CDS             2230..5061
                     /locus_tag="VGA59_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HEX9719442.1"
                     /translation="MAEPVNALLAIVVLLPLLPGVIIAYLAGRSPEPRRRRLAAWLTG
                     TIAAAALMVLLALAPAVFAGQTLLMHVDWVPQIGLNIGLRLDGLALMFGLLITGIGVL
                     IVLYAAYYLGPNDPAGKFYALLMLFMAAMLGIVLADNLLLLVVFWELTSISSYLLVGY
                     WGHRADARAGARMALAVTGGGGLAMLAGIILLGQAGDSYELSVLLERGDLIRSHPQYA
                     WILALILLGCFTKSAQVPFHFWLPEAMAAPTPVSAYLHSATMVKAGVFLLARLYPVLG
                     GTDAFQFWVSGTGIATLAFAAYVAVFKHDLKGLLAYSTISHLGLIVFLLGLNSPLAKV
                     AAVFHVLNHATFKASLFMTAGIIDHETGSRDMRKLGGLYRFMPWTATLAMVAAASMAG
                     VPLVNGFLSKEMFFDETLRAGGGASSFIVPAIATFAGICSVAYSLRFIHDVFFHGPPQ
                     GLPNPHPHEPPMFMKLPVAVLVVLCVVVGLFPAATFGPIVQLAAAAVLHTAPPEYHLA
                     LWHGFNEPLLMSSLALAGGVAMYVALQRQFNLHLHHPPRVTGRLLFTRTIEGLFSAAR
                     RFTGWTETGSLQRNAALLIAAAVLVASWPLLRSEAPMAAGSRTLLPASLLAVVLWGIL
                     IAAGAALVLGHHDRLRGVVLAGVVGLITALSFEALSAPDLALTQLSVEVVSTALLLMG
                     LALLPRSTPRESSGLRRTRDALLAVAGGGGVAWLAWLALTRDHNSLSWYFLENSVPQG
                     GGANAVNVILVDFRGYDTFGEITVLAIAAVGVLAMLEGWRVRRPTLDPAGLPWSFAQP
                     SLMLRLAARLILPLALMVSAYIFWRGHNLPGGGFIAGLITAAALVLQYMALGQSAAEA
                     MLGGDTGRRFTWWIASGLAIAGLTGAGALLFGRPFLTSANAHRTLPLLGEMGLATAAL
                     FDLGVYITVVGATMLLFATLAAASKEPHR"
     gene            5058..5414
                     /locus_tag="VGA59_06885"
     CDS             5058..5414
                     /locus_tag="VGA59_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HEX9719443.1"
                     /translation="MNLSLEFLVASGVGWTTACGIYLMLRGRTFPVVLGLTLLGYAVN
                     VFIFAMGRLWVGMPPILGGARPAADPLPQALVLTAIVIGFATTGLVIEMALRSRHESG
                     SDHVDGLPPSGERDPG"
     gene            5411..7018
                     /locus_tag="VGA59_06890"
     CDS             5411..7018
                     /locus_tag="VGA59_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HEX9719444.1"
                     /translation="MIVLTQWLDAHLAVLPVLLPLLTAALLLALGDGAGSHGSGALAR
                     ARLISLSSVGLGFIVAAQLVIDADVGTWRVYHLGEWPAPFGIVLVVDRLSALMVLLTQ
                     LLAVPIVLYASGGWDTHGRHFHALLHFQLMGLSGAFVTGDLFNLFVFFEVLLIASYVL
                     LVHGQGRERFRFGVPYVVLNLVASALFLFGVALIYAVTGSLNLADLALRVGRVTGTEA
                     ALLQAGAMLLLVVFGFKAALLPLHLWLPGTYAAASPPVAALFALMTKVGVYAILRVHG
                     VVFGDGAGESARVVEPWLLPLALASSLAAVLGALAAQTLPRLVAWLTIASVGTILAAV
                     GLFSTAAWSAAIYYLAQSTLVIAALFLLSELVGAQRGEVGDRLESAGPVAQPALLGTM
                     MLLLAASVIGLPPLPGFLGKLMILQAASGPAMPWLWSVVLLVGFLSLLGLARAGGILF
                     WAVDPARQGGASGASPQLLAATMSLLFALLVMTGAAGPFKRYTDAAADQLVDREGAAR
                     AVLGGGPGARPDAVRRYRPEDAQGVKP"
     gene            7015..>7350
                     /locus_tag="VGA59_06895"
     CDS             7015..>7350
                     /locus_tag="VGA59_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX9719445.1"
                     /translation="MSPADPRDDTAARTARRRWLAHPWLSSMIVLAWVLLQGSLAPVH
                     LIWALIMGLGLPWLLHDFLGAGTRLRAGKLALRLAFVVLWDIVVANLAVARIVLHPGV
                     EPQPAWIRVP"
BASE COUNT          941 a         2433 c         2559 g         1417 t
ORIGIN      
        1 gatcgaagtt ttcgacgaac cggaagtggc cgccgccaga catcgcgccg ggctgcggcg
       61 cctgttcgcg ctgcagatcc gggacgcgct gaagtacctc gaaaagaacg taccggacct
      121 gcagaagatg gcggtcgcct acatgccgct gggaacgcag gaggaactgc gcgagcagat
      181 catcgaagtg gcgctggatc gcgcttttct gcagccgccc ctaccctcga acgaggccga
      241 cttcaagcgc cggatcgacg agggcagagc gcgcctgacg ctcattgcga acgaagtggc
      301 gcggcttggc gccgccatcc tggccgaata cgcgaccgcc gtgcgcaaga tcagggacac
      361 gaagaaccag ccggacgcca ccggcgacgc gacggggcag ctgcagcgcc tgatgcccaa
      421 acgtttcctg gccgtcacgc cgtggaccca gctgcagcat tttccgcgct acctgaaggc
      481 gatcacgctg cgcctggaca agctgcgctc cgatccggcg cgcgatgccg ccaggctggc
      541 ggagctcaag ccgcaggagc agcgctactg gcgtctcgtg gccgagcgca agggcgcagt
      601 ggatgaacgg atgcaggaat accgctggct gctggaagaa ctgcgggtga gcttcttcgc
      661 gcaggaactg cgtacgcccc agcccgtgag cgtcaagcgg ctggacaagt tatggagtca
      721 gctcaatagc tgacctatgc ttcggctcgc ggttgccgct atatcttgcc catcagcagc
      781 agcacgagca ggatcaggac caccaggccg aggccgccgg aagggccata gccccagttc
      841 cggctgtggc cccagctggg cagggcgccg accaacagca ggatcaacag gatcagcagg
      901 atcaacgaga tcgacatggc tttctccttg tgtcgcatcc cgactctggc atgaccggca
      961 gacgcagcgg gtcagcggtg gccccgggtc acggtaaggc agctcctacc gttgtgtggg
     1021 ctcaggcgcc gcgaggtgac ccggcggata aactggagca aacaccatga gcacctaccg
     1081 ttgatccggg cgcagttcct gctcttcctg ctatgtgtcg ctgcccgcgg ccgatggtcc
     1141 agtcattttt cagcaggcgg atgagccgca caatggcggc atgacacttg cctctctcgc
     1201 gctgcaattc gccgtttgtg cgctgctgat cgcacgagcc gggttcgtgc tcagcagcag
     1261 cgctgaccgc ctggccggta catacggctg gggccgcggc tgggttgggc tgacgctgct
     1321 ggccacggtg acgtcgctac ctgagctcgc ttcgggcatc agcgcggtgg ctttcgtcga
     1381 tgcgcccaat ctcgcggtgg gcaatgcgct cggcgcctgt gtcgtgaatc ttgcctttct
     1441 ggtcgttgtc gacgtgctgc agcgcaacca gccgatgtac cgcgacgcca gcgcgactca
     1501 cctgctgtcg gccggcttcg gcgtggtgat gctcggcttc attgcgatga gcctgctgac
     1561 cggcacacgc gcgccggcgg tattgcacgt gggcatctac agtcccctgc tgctggggct
     1621 ttacctgcta gcgctgcggg gcgtccacgc ccatgagcgc acggcgatgg cgggttgcgc
     1681 cgcggccgcg cacccggcgt ccgcaccctc ggacacacgg cgcgagtggc ggcgctttgg
     1741 cctcgcggcc ctgatagtgc ttgccgccgg cggttggctg cccgaagtgg ccgacgcgtt
     1801 ggcgcaaacg ctgggcctgt cgcgcagctt cgtcggcaca gtcttcatgg ccgtggtgac
     1861 cacgctgccg gagatcgccg tgaccgtctc ggcgttgcgg ctgggcgcgc ttgacatggc
     1921 gatcggcaac ctgctgggca gcaacctctt caacgtgctg atccttgcgg tggacgacac
     1981 tttctacacc aagggtccgc tgctcgccga cgcagccccg gtgcatgtcg gcacagcggt
     2041 gaccgcgctc gtcatgacgg gcctggtcat catcggcctg gtgatgcgtc cgcaagggcg
     2101 cgtgttgcgc gtcctgagct gggtcagcgt cggcctcgtg gccgcctacg tgatcaacgc
     2161 gtcgctggcc tatctgggcg gcgcttgagg ccgccgtcgg agcgcaagac gctgtttgac
     2221 cctcctacaa tggccgagcc cgtgaatgcg ttgctcgcca tcgtcgtcct gttgccgctc
     2281 ctgcctggcg tcatcatcgc ttacctcgcg ggccgctcgc ccgagcctcg tcgtcggcgg
     2341 ctcgctgcct ggctgacagg cacgatcgcc gcggccgcgc tgatggtgct gcttgcgctt
     2401 gcaccggccg tcttcgccgg ccagacgctg ctgatgcacg tcgactgggt gccgcagatc
     2461 ggcttgaaca tcggcttgcg cctggatggc ctggcgttga tgttcggcct gctgatcacg
     2521 ggcattggtg tgctgatcgt gttgtacgcc gcctactatc tgggcccgaa cgatcccgcg
     2581 ggcaagttct atgcgctgct gatgctgttc atggccgcga tgctgggcat cgtgctcgcg
     2641 gacaacctgt tgctgctggt ggtgttctgg gagctgacca gcatctcctc gtacctgctg
     2701 gtgggatact ggggccatcg ggccgacgca agggcgggcg cgcgcatggc gctggccgtg
     2761 accggcggtg gggggctcgc catgctggcc ggcatcatcc tcttggggca agcgggcgac
     2821 agctacgagt tgtcggtgct gctggagcgc ggcgatctca tccgcagcca cccacagtat
     2881 gcatggatcc tcgcgctgat cctgctgggt tgcttcacca agagcgcgca ggttccgttc
     2941 catttctggc tgcccgaggc catggccgcg ccgacgccgg tttcggccta cctgcactcg
     3001 gcgaccatgg tcaaggcggg tgtgttcctg ctggcgcgcc tgtaccccgt cctcggcggc
     3061 accgacgcct ttcagttctg ggtgtcgggc accggcatcg cgacgctggc gttcgcggcc
     3121 tacgtcgccg tgttcaagca cgatctgaag gggctgctcg cgtattcgac catcagccac
     3181 ctggggctga tcgtcttcct gctcgggctg aattcgccgc tggccaaggt ggcggccgtc
     3241 tttcacgtgc tgaatcacgc gaccttcaag gcctcgctgt tcatgacggc cggcatcatc
     3301 gaccacgaaa ccggctcgcg cgacatgcgc aagctcggtg gcctgtaccg cttcatgccc
     3361 tggacggcca cgctggcgat ggtggccgcc gcctcgatgg ccggtgtgcc gctggtcaac
     3421 ggcttcctgt ccaaggagat gttcttcgac gaaacgctgc gggcgggtgg cggagcctcg
     3481 agtttcattg tccccgcgat cgccaccttt gcgggcatct gctcggtcgc ctattcgctg
     3541 cgcttcatcc acgatgtgtt tttccacggc ccgccccagg gcctgccgaa tccgcatccg
     3601 cacgagccgc cgatgttcat gaagctgccg gtggcggtat tggtggtgtt gtgcgtggtc
     3661 gtggggctgt tcccggcagc caccttcggc ccgatcgtgc agctcgcggc cgccgcggtg
     3721 ctgcacaccg ccccgcccga atatcacctg gcgttgtggc atggcttcaa tgagccgctg
     3781 ctgatgagtt cgctggcgct cgcgggcggc gtcgcgatgt acgtggcatt gcagcggcag
     3841 ttcaacctgc atctgcacca tccgccgcgc gtcacggggc ggctgttatt cacgcggacg
     3901 atcgaagggc tgttctccgc ggcacgccgc ttcaccggct ggacggagac aggttcgctg
     3961 cagcgcaatg cggcgctgct catagcggcc gccgtactgg tggcgtcgtg gccgctgttg
     4021 cgttccgagg cgccgatggc ggccggcagc cgcacgctgc tgccggcatc gctgctggcc
     4081 gtcgtgctat ggggcatcct gatcgccgcc ggggctgcgc tggtgctggg ccaccatgac
     4141 cgcctgcgcg gcgtggtgct ggccggcgtg gtcggcctga tcaccgcgct cagcttcgag
     4201 gccctgtcgg cgcctgacct tgcgctcacg cagctttcgg tggaggtggt gtccacggcg
     4261 ctgctgctga tgggcctggc cttgctgcca cgctccacgc cacgcgaatc cagcggcctg
     4321 cggcgcacgc gcgatgcact gctcgccgtg gcgggcggcg gcggtgtggc ctggctcgcc
     4381 tggctggcat tgacacggga ccacaattcg ctgtcgtggt acttcctcga aaattccgtg
     4441 ccgcagggtg gcggcgcgaa tgcggtcaac gtgatcctgg tggacttccg cggctacgac
     4501 accttcggcg agatcaccgt gctcgccatc gccgccgtgg gcgtgctggc gatgctggaa
     4561 ggctggcgcg tgcgccgccc gacgttggat ccggcgggcc ttccgtggtc gtttgcgcaa
     4621 ccgtcgctga tgctgcgact ggccgcgcgc ttgatcctgc cgctggcgct gatggtgtcg
     4681 gcctacatct tctggcgcgg ccacaacctg cccggcggcg gtttcatcgc cgggctgatc
     4741 accgcggccg cactggtgct gcagtacatg gcgctggggc aaagcgcggc ggaggcgatg
     4801 ctcggtggcg ataccggccg gcgcttcacg tggtggatcg cgagcggcct ggcgatcgcg
     4861 ggcctcaccg gcgccggggc cctcctgttc gggcggccct tcctcaccag cgccaatgcc
     4921 caccggacgc tgccactgct cggtgagatg gggctcgcga ccgcggcgct attcgacctg
     4981 ggcgtctaca tcacggtggt gggcgccacc atgctgctgt tcgccacgct cgccgcggcc
     5041 agcaaggagc cccacaggtg aacctcagcc tggagttcct cgttgcctcc ggcgtcggct
     5101 ggaccaccgc ctgcggcatc tacctgatgc tgcgcgggcg caccttcccg gtggtgctgg
     5161 gcctgaccct gctggggtac gccgtcaatg ttttcatctt cgccatgggc cggctgtggg
     5221 tgggaatgcc gcccatcctt ggcggcgcgc gaccggcggc cgatccgctg ccacaggcgc
     5281 tggtgctgac cgcgatcgtg atcggattcg ccaccacggg cctggtgatc gaaatggcgc
     5341 tgcgcagccg gcatgagtcg ggcagcgatc acgtcgatgg cctgccaccc tccggcgaaa
     5401 gggacccggg gtgatcgtgc tgacgcaatg gctcgatgcg caccttgcgg tgctgccggt
     5461 gctgctgccc ttgctcacgg ccgcgttgct gctcgccttg ggcgacggtg ccggttcgca
     5521 tggcagcggg gccctggccc gggcgcggct gatctcactg tcgtcggtcg ggctcgggtt
     5581 catcgtggcg gcgcagctgg tgatcgacgc ggatgtcggc acctggcgtg tctaccacct
     5641 gggcgagtgg cccgcgccct tcgggatcgt gctggtagtc gatcggctgt cggcgctgat
     5701 ggtgctgctg acccagctgc tggcggtgcc catcgtcctg tacgccagtg gcggctggga
     5761 cacgcatggc cgccactttc acgcgctgct gcactttcag ctgatggggc tcagcggtgc
     5821 gttcgtcacc ggggacctgt tcaacctgtt cgttttcttc gaggtgctgc tgatcgcgtc
     5881 ctatgtgctg ctggtgcatg gccaggggcg cgagcgcttc cgtttcggcg tgccctatgt
     5941 ggtgctgaac ctggtcgcct cggcactgtt cctcttcggc gtggccctga tctacgcggt
     6001 gacgggaagc ctgaacctgg ccgacctggc gctgcgggtg ggccgggtga ccggtaccga
     6061 ggcggctttg ctgcaggccg gcgcgatgct gctgctggtg gtgttcggct tcaaggcggc
     6121 gctgctgccg cttcacctgt ggctgccggg cacgtatgcg gctgccagcc cgcctgtggc
     6181 ggcgctgttc gcgctgatga ccaaggtcgg cgtgtacgcg attcttcggg tccacggcgt
     6241 cgtcttcggc gacggggcgg gcgagtcggc acgcgtggtg gaaccctggc tgttgccgct
     6301 cgcgctggcg agcagcctcg cggcggtgct gggtgccttg gccgcgcaga cgctgccgcg
     6361 cctcgtcgcc tggctgacca tcgcctcggt cggtaccata ttggcggcag tcggcctgtt
     6421 cagcacggcg gcctggtcgg cggccatcta ctacctggcg cagagcacgc tggtcattgc
     6481 ggcgcttttc ctgctgtcgg agctggtggg cgcacaacgc ggcgaagtgg gcgaccggct
     6541 cgagtcggcc ggcccggtgg cgcaaccggc gctgctgggc acgatgatgc tgctgctcgc
     6601 cgcttcggtg atcgggctgc caccgcttcc cggcttcctg ggcaagctga tgatcctgca
     6661 ggccgcgagc ggacctgcga tgccctggct gtggagtgtc gtcctgctgg tcggcttcct
     6721 gtcccttctg ggcctggcac gcgccggcgg tattctgttc tgggcggtcg atcccgcgcg
     6781 gcaggggggc gcgtccggag ccagcccgca gttgctggcg gccacgatgt cgctgctgtt
     6841 cgcgctgctg gtcatgacgg gtgccgcggg tcccttcaag cgttataccg acgccgcagc
     6901 cgaccagctc gttgaccgcg aaggcgccgc ccgcgcggtg ctgggcggcg gcccgggtgc
     6961 cagacccgac gccgttcggc gctataggcc cgaagatgcc caaggagtca agccatgagc
     7021 cctgccgacc cacgcgacga cactgccgca aggacagcgc ggcggcgctg gctcgctcat
     7081 ccgtggctgt cgtcgatgat tgttcttgcc tgggtgcttt tacaggggtc gctggcaccg
     7141 gtccacctga tttgggcgct gatcatgggg ctggggctgc cctggctgct gcatgacttc
     7201 ctcggtgccg gcacgcggct gcgcgcgggc aagctggcgc tgagactcgc cttcgtcgtg
     7261 ctctgggaca tcgtggttgc caacctcgcg gtggcgcgca tcgtgctgca cccgggcgtc
     7321 gaaccgcagc ccgcctggat ccgtgtgccg
//
LOCUS       DASWIY010000185         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2696731, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000185 DASWIY010000000
VERSION     DASWIY010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2696731"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1215
                     /gene="waaF"
                     /locus_tag="VGA59_06900"
     CDS             199..1215
                     /gene="waaF"
                     /locus_tag="VGA59_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX9719446.1"
                     /translation="MSGSLVIAPQWIGDAVMTEPLLRRLRTRGERLAVGALPWVAPVY
                     RAMPQVDEVIEFPFQHGGLQFKARRGIARQVEGRFDTAYVLPNSLKSALLPFLAGIPK
                     RVGYLGEARVGLLTHRLKNPKDKPPMVAFYSALSGDREGLENDRPQLQLPAAHVTAAL
                     AALELQAGGYYVFAPGAEYGPAKRWPAIHFAELARALDAPVVLLGSGKEADLCQEIAG
                     AAGHGGCLNLAGKTALLQAFALIAAARAVISNDSGLMHVAAAFGVPQVAVFGSSSPLH
                     TPPLNDKAQVLWLKQDPAYQPPLDCSPCFQRECPLGHTRCLYDLTPEVVIEALSREPS
                     HEPR"
     gene            complement(1455..2645)
                     /locus_tag="VGA59_06905"
     CDS             complement(1455..2645)
                     /locus_tag="VGA59_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9719447.1"
                     /translation="MTPWRARFRFPLYLRIWLAVVVAVAVLTIAFGWLWRLNAAQSPP
                     REVIIRNEAGDILGQDRTRPIREPGQPTEYQVTMNDGSVLVVQFPRRARGPGGALSGR
                     PWWVRGPGGLLWILGIVALAVAVGTYPIIRRLTQRLDDLRLGVERWGEGDLSARIKES
                     GSDELAFLAQRFNNAAERIETLIESHKSLLANASHELRSPLARIRMGLELMEPEGSAA
                     FREEIQRNIAELDQLIDEILLASRLDAKESDIGTVESVDLTGLAAEECARAGAELVPI
                     QDGQSLVVRGVAKLLRRAIRNLLENARRYSSGPVTVELAREGDKAVVRVRDRGPGVPP
                     GERERIFEPFYRLAGASERHGGVGLGLALVRSITQRHHGTVQCEDQEGGGACFTVRIP
                     GHQT"
BASE COUNT          471 a         1012 c          985 g          488 t
ORIGIN      
        1 gagcccagcc cgcaatgaca gttcacccaa cagttgtcat gccgggcttg acccggcatc
       61 catgacttcc tggattgcgg ggccggaccg gagcgtgatg cctcggcacg tgcatagtgc
      121 aggtccggca ttgggctcgc tgcactggac agctgcgcgc cccaggcgtt ccgcgagccc
      181 agcccgcaag gtcagacctt gagcggctca ctcgtgatcg ccccgcagtg gatcggcgac
      241 gcggtgatga ccgagccgct gctgcgccgg ctgcgcacgc gtggcgaacg gctcgccgtg
      301 ggtgcgctgc cctgggtcgc gcccgtgtac cgcgccatgc cgcaggtcga tgaggtcatc
      361 gagtttccat tccagcacgg cggcttgcag ttcaaggcgc ggcgcggcat cgccaggcag
      421 gtcgaagggc gattcgacac ggcctatgtc ctgccgaact cgctcaagag cgcgctgctg
      481 ccgttcctgg ccggtattcc caagcgggtg ggctacctcg gcgaagcgcg ggtcggcttg
      541 ctcacgcacc ggctgaagaa tcccaaggac aagccgccga tggtggcctt ttactcggcc
      601 ctcagcggcg acagggaagg gctggaaaac gaccggccgc aactgcaatt gcccgcggcg
      661 cacgtgaccg ccgccttggc cgcgctggaa ctgcaggcgg gcggttacta cgtgtttgcg
      721 ccgggagccg agtacggccc ggccaagcgc tggccggcca tccatttcgc ggaactcgcg
      781 cgcgccctcg atgcgccggt ggtcctgctc ggctcgggca aggaagccga tctgtgccag
      841 gagatcgccg gcgcggccgg ccacggcggc tgcctcaatc tcgccggcaa gacagcgttg
      901 ctgcaggcgt tcgcgctgat cgccgcggca cgcgccgtga tcagcaacga ctccggcctg
      961 atgcacgttg cggcggcgtt cggcgtgccg caggtggcgg tgttcggctc ctcgagcccc
     1021 ttgcacacac cgcccttgaa cgacaaggcg caggtcctct ggctcaaaca ggaccccgcc
     1081 taccagccgc cgctggattg ctcaccgtgc ttccagcgcg aatgcccgct ggggcatacc
     1141 cgctgcctgt acgacctcac gccggaggtc gtcattgagg ccttgagccg cgaaccatcc
     1201 catgaaccgc gctgacaaaa aacagccacc ttgcggcggc tgtataaaag tcctcggaag
     1261 gacgtcgttg ggagactatg aaacttcttg cggctgcgtt ggccctgagc gacagctggt
     1321 ttgactataa gtgccgccgc ccgctggtgc atcccccgaa tgggggagac gcttccaata
     1381 cccgccccgt aaaatggtta tcttggagat ttttcgcgcg gctggcgagc gtttccaatt
     1441 tttattacag cgcattacgt ctggtgaccg ggaatgcgta ccgtgaagca ggcaccgccg
     1501 ccctcctgat cctcgcactg gacggtgccg tggtgccgct gcgtgatgga acgcaccagg
     1561 gccagtccca ggccgacacc gccatggcgc tcgctggcgc cggccagccg gtagaacggc
     1621 tcgaagatgc gctcgcgctc tcccggggga acccccggtc cgcgatcgcg cacgcgcacc
     1681 acggccttgt cgccttcgcg cgccagttcc accgtgaccg ggccgctgct gtagcggcgg
     1741 gcgttctcca gcagattgcg gatcgcacgc cgcaagagct tggccacgcc tcgcaccacc
     1801 agcgactggc cgtcctggat gggcaccagc tcggcgccgg cgcgtgcgca ctcctcggcg
     1861 gccagcccgg tcaggtcgac cgactccacc gtgccgatgt cgctttcctt cgcgtcaagg
     1921 cgactggcca gcaggatctc gtcgatgagc tggtcgagtt cggcgatgtt gcgctggatc
     1981 tcttctcgaa aggcggctga gccctccggc tccatcagct ccagccccat gcggatgcgc
     2041 gccagcggcg agcgcagctc gtgcgaggca ttggccagca gcgacttgtg cgattcaatg
     2101 agggtctcga tgcgctcggc ggcgttgttg aagcgctgcg ccaggaacgc cagttcgtcc
     2161 gagcccgatt ccttgatgcg cgccgacagg tcgccctcgc cccagcgctc cacgcccagt
     2221 cgcaggtcat cgagccgctg ggtcagcctg cggatgatcg ggtacgtgcc tacggcgacc
     2281 gccagggcca cgatcccaag gatccacaac aggccgcccg ggccgcgcac ccaccagggc
     2341 cggccggaca gggccccccc agggccgcgc gctcgacgcg gaaactgcac gaccagaacg
     2401 ctgccgtcgt tcatggtcac ctggtactcg gtcggctgcc cgggttcgcg aatcgggcgc
     2461 gtccggtcct ggccgaggat atcgccggct tcgttgcgga tgatcacctc gcgtggcggc
     2521 gattgtgcgg cattcaagcg ccacagccag ccgaaggcga tcgtcagcac ggccaccgcc
     2581 acgaccacgg ccagccagat acgaaggtag agagggaaac ggaaacgagc ccgccatggc
     2641 gtcatctgaa ttccttcgcg gcgccatgca tcgcctttgg catgtgaaaa ctgagggtcc
     2701 gtgcgtgggt caccgcggaa ccggctttgc cgggccgctg gtgggccttg caggccgcat
     2761 ggccgcgaga cgcggccatt cctcgacctg tccaaaccga agcaggtcgg tttggagccg
     2821 caggtctcgg ccccctcgag ggggagaggg cgaagcccga gggggcatcg ccgtaggcgc
     2881 ttcgggggtg ggtgttcact cctgctgctt ggcgaagacg tagcccaccc cgcgcacggt
     2941 gagaatgcgc ttgggg
//
LOCUS       DASWIY010000186         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1591904, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000186 DASWIY010000000
VERSION     DASWIY010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591904"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VGA59_06910"
     CDS             complement(<1..345)
                     /locus_tag="VGA59_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX9719448.1"
                     /translation="MLIEVKVPQLSESVAEATLLAWHKKQGEAVKRDENLIDIETDKV
                     VLELPSPADGVLVSILKGDKGTVKAGEVIAKIDTEGKAVSGVSPIAVPAEGAKPAAAP
                     AKSAAPAVMPAAK"
     gene            complement(335..>1825)
                     /gene="sucA"
                     /locus_tag="VGA59_06915"
     CDS             complement(335..>1825)
                     /gene="sucA"
                     /locus_tag="VGA59_06915"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587416.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX9719449.1"
                     /translation="EPMVTQPLMYKIVNAHPGTRKLYADRLLAQGVLAAEEADQLVAN
                     FRQALDGGHHTNKTILSDFKPPFAVDWSKYNKSKWNEKDDTQLPLAELQALAERLTSI
                     PPGFKLHPRVEKIIADRRLMGQGKMPVDWGMAENLAYASLLKDGYAVRISGQDSGRGT
                     FFHRHAVLHDQNREKFDAGTYVPLAHIAPNQGDFVVIDSVLSEEAVLGFEYGYSTSEP
                     NELVVWEAQFGDFVNGAQVVIDQFIASGEVKWGRICGMVLMLPHGYEGQGPEHSSGRI
                     ERFLQLCADYNLQVCMPATPVQLFFLLRRQMIRPYRKPLVIFTPKSLLRHKESVSPLE
                     EFANGKFKPINTEWNDDVKPEQVTRVVFCSGKVFFDLLAKRKELAREDVAIIRIAQLY
                     PFPHDDFQATIDRYAKAYEVVWCQEEPGNQGAWHRIQHYLLRHMRQEQVLAFAMRKSS
                     ASPAVGYLSLHNEQQKELVATAFAPIGDNLVLPVQGTARRGGSDVD"
BASE COUNT          283 a          606 c          586 g          350 t
ORIGIN      
        1 tttcgcagcg ggcatcactg cgggagcggc actcttcgcg ggtgcagcgg ctggtttcgc
       61 gccttccgca ggaacggcga tcggggaaac cccggaaacg gccttcccct ccgtgtcgat
      121 cttcgcgatc acctcgcccg ccttcaccgt gcccttgtcg cccttcagga tgctcaccag
      181 caccccgtcg gcggggctgg gcagttcgag caccaccttg tcggtctcga tgtcgatcag
      241 gttttcgtcg cgcttcaccg cttcgccctg cttcttgtgc caggcgagca gcgtcgcctc
      301 ggccaccgac tcggagagct gcggcacttt cacttcaatc aacatccgaa ccacccctcc
      361 tggcggtgcc ctgcacgggg agcaccaggt tgtcgccgat cggcgcgaag gccgtggcca
      421 ccagctcctt ttgctgttcg ttgtgcaacg acaggtagcc caccgcgggc gaggcggagg
      481 acttgcgcat ggcgaaggcg agcacctgtt cctggcgcat gtggcgcagc aggtaatgct
      541 ggatgcgatg ccacgcgccc tggttgccgg gctcttcctg gcaccacacc acctcgtagg
      601 ccttggcgta gcggtcgatg gtggcctgga agtcgtcgtg cgggaacggg tagagctgcg
      661 cgatgcggat gatggcgaca tcctcgcgcg cgagctcctt tctcttggcg agcaggtcga
      721 agaacacctt gccgctgcag aacacgaccc gcgtcacctg ttcgggcttg acgtcgtcgt
      781 tccattccgt attgatcggc ttgaacttgc cgttggcgaa ctcttccagc ggcgacacgg
      841 attccttgtg ccgcagcagg ctcttcggcg tgaagatgac cagcggcttg cggtaaggcc
      901 ggatcatctg ccggcgcagg aggaaaaaca gctgcaccgg cgtggccggc atgcagacct
      961 gcaggttgta gtccgcgcac agctgcagga aacgctcgat gcggcccgag gaatgctccg
     1021 ggccctggcc ctcgtagccg tgcggcagca tcagcaccat gccgcagatg cggccccact
     1081 tcacttcgcc cgaggcgatg aactggtcga tcaccacctg cgcgccgttc acgaagtcgc
     1141 cgaactgcgc ttcccacacc accagctcgt tcggctccga ggtggaatag ccgtactcga
     1201 agccgagcac cgcttcttcc gagagcaccg agtcgatgac cacgaagtcg ccctggttcg
     1261 gcgcgatgtg cgcgagcggc acataggtgc cggcatcgaa cttttcgcga ttctggtcgt
     1321 gcagcacggc gtggcggtgg aagaacgtgc cgcgtccgct gtcctggccc gagatgcgca
     1381 ccgcatagcc atccttgagg agcgaggcgt aagcgagatt ctcggccatg ccccagtcca
     1441 ccggcatctt gccctgcccc atcaggcgcc ggtcggcgat gatcttctcg acgcgcgggt
     1501 gcagcttgaa gccgggcgga atcgacgtca gccgctcggc gagcgcctgc agctcggcga
     1561 gcggcagctg cgtgtcgtcc ttctcgttcc acttcgactt gttgtacttg ctccagtcga
     1621 cggcgaacgg cggcttgaag tcggagagga tcgtcttgtt ggtgtggtgg ccgccgtcga
     1681 gcgcctggcg aaagttcgcc accagctggt cggcctcttc ggccgccagc acgccctgcg
     1741 ccagcagccg gtcggcgtag agcttgcgcg tgccggggtg cgcgttgacg atcttgtaca
     1801 tcagcggctg cgtcaccatc ggctc
//
LOCUS       DASWIY010000187        11352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_842488, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000187 DASWIY010000000
VERSION     DASWIY010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11352
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842488"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..140
                     /locus_tag="VGA59_06920"
     tRNA            56..140
                     /locus_tag="VGA59_06920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:90..92,aa:Leu,seq:cag)
     gene            complement(448..1512)
                     /gene="fba"
                     /locus_tag="VGA59_06925"
     CDS             complement(448..1512)
                     /gene="fba"
                     /locus_tag="VGA59_06925"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206893.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX9719450.1"
                     /translation="MSLVSMRELLDHAAENGYGIPAFNVNNLEQVQAVMEAAKETGAP
                     VILQASAGARKYAGESFIKHLILAAIEAYPAIPLVMHQDHGQSPDVCQGAINLGFSSV
                     MMDGSLQADGKTIASYDYNVDVTRKVVDMAHKAGVTVEGELGCLGSLETMKGDKEDGH
                     GTESTMTREQLLTDPEQAADFVKKTQLDALAIAIGTSHGAYKFTRKPTGDILAIDRVK
                     AIHKRIPNTHLVMHGSSAVPQDLLAVIRQYGGNMKETYGVPVEEVQEAIKHGVRKINI
                     DTDIRLAMTGAVRKFLAENPEKFDAREWLKPAREAAKQICKQRYIQFGCEGQAAKIKG
                     QSLQVVASRYAKGELAQVVR"
     gene            complement(1708..3141)
                     /gene="pyk"
                     /locus_tag="VGA59_06930"
     CDS             complement(1708..3141)
                     /gene="pyk"
                     /locus_tag="VGA59_06930"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX9719451.1"
                     /translation="MARRATKIVATLGPASSDPALLDQMIRAGVNVVRLNFSHGTAQD
                     HIDRAALVRAASQRAGREVAIMADLQGPKIRVGKFADGKVALEPGMKFVLDASRSEPG
                     DANGVGLDYKELPRDVKAGDLLLLNDGLIVLTVDAVRGEQVRTTVKQGGELSNNKGIN
                     KKGGGLTAPALTAKDMEDIRTAMNFQADYVAVSFPKNATDMEMARQLCNVAGAEFRHR
                     PGLVAKIERAEAIPNLESILKASDAIMVARGDLAIEVGNAAVPALQKRMIRMAREHDK
                     VVITATQMMESMINNPVPTRAEVSDVANAVLDGTDAVMLSAETATGRFPLETIVEMAN
                     ICLEAEKAEDVKLDADFTGMTFGRIDQSIAMGALFTAHHLGCKAIVALTDSGSTPLWM
                     SRHRIHIPIYALTPKLVTQRKMALYRNVRPLLLDQSAERDTALGEAEAHLKKRGIVAS
                     GDIYAITCGEPMGAPGGTNMLKICRAS"
     gene            3386..4111
                     /locus_tag="VGA59_06935"
     CDS             3386..4111
                     /locus_tag="VGA59_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9719452.1"
                     /translation="MRTQPEPTRRAFLAALPAVTAGLASPAAASQEEVPRWGMLIDTR
                     RCIGCQACTIACSMENVLPEGQFRTSAATYAVTNGKGQTGMSMLPRLCNHCEEPPCIP
                     VCPVGATFKGKDGVVLVDADRCVGCGYCVQACHYEARFINHDTGKADKCTFCSHRVEA
                     GLLPACVETCVGGARVFGDLNDPASTISRRMQAAQGKTSVLKPEAGTRPSVFYIGLDK
                     ELNGRVTGASATEMMWRPNQQGA"
     gene            4114..5208
                     /gene="nrfD"
                     /locus_tag="VGA59_06940"
     CDS             4114..5208
                     /gene="nrfD"
                     /locus_tag="VGA59_06940"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX9719453.1"
                     /translation="MNTTVEILGFTREPGWLPWAVQYFFLIGISAAAFFISLPGLAWR
                     HPEWRGVSRRALLAALVCGLTAPVALLADLHQPGRFLNFYLHPNLQSWMAWGSFFIPL
                     YLFGLLLYAWLCLRPQLAGLAREHEAGTRLAKLYRALAYGGHDNRGAIRVAAVLAALG
                     AVLVLLYTGMEVMVVRARPLWNTPLLPPLFAVTAFAGGLGMTALFEALSGHRDSAPLL
                     NRWAARSQWAVLALLAAWLLSAFTGIAPAAAEALASLRGSVSWRVTLGSLVAVSVLTL
                     WVARKRRDSLVLMALLALLGAWLLRWALIMGGQGIPKMGATFRSYYPTLTPDSALGIV
                     GTAGLFLTLYIVLTSFVPWDESTQAAGAAS"
     gene            5205..8291
                     /locus_tag="VGA59_06945"
     CDS             5205..8291
                     /locus_tag="VGA59_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX9719454.1"
                     /translation="MKISRRRQVLALGGIAAFGAGFSETAGRMAGKLMGRDKPTHKTA
                     GNAPTPEFKVDSQGNVAINTAQQVSYTTCLGCTTQCGVRVRTDRATGKVLRVAGNPYS
                     PMSTDPHLPMKASVRESFAAISAFEGKGLAGRSTACGRGNAVAQQIDSPYRVLTPLKR
                     VGARNSGRWEPISFEQLVKEVVHGGNLFGEGPVEGLAALRALDQPIDSNKPELGSRVN
                     QVAVLSSVNDGRESFARRFFQKSYGTLNFLGHGSYCGGAYRSGTGALFGDLKAMPHGK
                     PDFANAEFVLFVGTAPGQAGNPFKRQGTLVAKARTEGKLSYVVVDPVLTHASSRASGD
                     RGRWLPIRPGTDGAMAMAIIRWLIENERIDSRFLGFPNAATARENGEPSFSNATHLVV
                     VEPKHPREGRMLRASDLGAAMTEEQRYKEADAFVCLDAAGKAVLHDKAKGPAQLFVDT
                     VLQVEGKDVRVKSSLQLLKEEAMRLDLSAYAAACGIEAATLTALASELASHGKRASVI
                     AHGGMMSGSGFYNAYALMSINALLGNINWKGGMVANGGGFKPDGEGPRYNLDTFAGMV
                     KPGGTPLGRNVPYEKTSEFAARKADGKPYPAKAPWFPNAPGLTTELLPGGISGYPYSL
                     KALILWSSNPMYGIPGLRERIAKDLADPAKLPLIVSIDPFINESNAYADYIVPDSLMY
                     ESWGWVAAWNGVPTKAMGARWPVMEPKAQKTPEGRAIGMETFFIALARAMSLPGFGSG
                     AITDTDGNDYALDGPEDWYLRAGANIAWLGQEPVGNASEEDILLSGVHRLRSVLERTL
                     KAEEVAKVAFLLSRGGRYQPAKDAYDEENAEWMRNQLKPMCHLWNENVGGSKNSLSGK
                     RNRGCATWLEPAFADGTPMRKVYPEKQWPLQLVSYKSALQNPYSIGATRLLAVHPENP
                     VLIHPDDAQRLGLAMGDLAEITTPGGSLKARVMVHAGIAAGVAAFEHGFGHRELGARA
                     HRIGGKHQPEDRRLAAGVNLNDIGLMDPMRPDRAPWVDAISGSSVRNGLPAKIRPA"
     gene            complement(8278..8928)
                     /locus_tag="VGA59_06950"
     CDS             complement(8278..8928)
                     /locus_tag="VGA59_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719455.1"
                     /translation="MNYQPDQCIVQVVDDDAGLRRSLRFLLESVDWKVQLHASAEEFL
                     EAVANPTQPTCVLLDIRMPAMSGLELQQVLRQRGIDIPILFMTGHADVSMAVQAMKSG
                     AADFIEKPFKDQVLLDAVAAAMRRCAESLQERQVRDEAIALLAGLSPREREVARLVAQ
                     GQPNKLIAAALGISEKTVHIHRQHVMEKARVSSAAELARLMLRVDPAALDDAIRPA"
     gene            complement(8912..10735)
                     /locus_tag="VGA59_06955"
     CDS             complement(8912..10735)
                     /locus_tag="VGA59_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX9719456.1"
                     /translation="MILVKLRWRRNANSRGGMFSITRLLFAAALAFWANEAPARDVSI
                     GVFAYQGERAASEDWSAVLDYLNAALPEHRFRLDHHDAAGLRKAIAEHRVDLVVTNPG
                     YYITMEGEFGLSRIATLSSPHGTPDRAIGSAVFARADRAELRELSALAGKRVAAVAPE
                     AFGGYLVAAREMLRQGVDTESDLEELRFVGLPMARVVEAVQRGEVDAGIVRVCVLEQM
                     AGRGELSLASFRILSPTRIDGFACALSTQLYPDWPIAVTRQTDPALAKTVARALLAMP
                     EREGSMSWAVPADYQPVHELYRELRVGPYASLRDVTPAGLAKRFWPWLAGVLVLLVAW
                     VVHTVRVEYLVHRRAAQLQESMQARQQAEMRITESQEQMEHLSRLSVLGELSGNLAHE
                     INQPLTTIGTYARSVLRRQSGGTLTPEAVTEACTEIASEAERAGGIVQRIRHFARKRV
                     AARDPVDLASIAQEARRLISGMLARAPDIAVEDRRQQVCRVLADGPQIQQVLLNLIKN
                     AIDSSRDLPAERRNIKVAIEAAGSNLVVHVLDHGVGLDAAQRARLFQPFFTTKPDGLG
                     LGLPICKTIIEAHGGRLWAEPNPDGQGMRFSFSLPCNELPA"
     gene            complement(10824..>11352)
                     /gene="pgk"
                     /locus_tag="VGA59_06960"
     CDS             complement(10824..>11352)
                     /gene="pgk"
                     /locus_tag="VGA59_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9719457.1"
                     /translation="ANTFMLAAGLKIGKSLAEVDLVSEARAVIEAMKARGAAVPIPTD
                     VVTAKAFAADAPAQVKAATDVADDDLILDIGPQTARKLAEQLKSAGTIVWNGPVGVFE
                     FDAFANGTETIARAIAQSPAFSIAGGGDTLAAIAKYGIEKNVGYISTGGGAFLEVLEG
                     KTLPAFEILEKRANG"
BASE COUNT         1879 a         3729 c         3728 g         2016 t
ORIGIN      
        1 cgctgtgctg atgtgtccaa tatttttgat ataaaatcct tttctttcct gaaatgccca
       61 ggtggcggaa ttggtagacg cactagtttc aggtactagc gagtaacatc gtgggggttc
      121 gagtcccttc ctgggcacca acaaacatca taaagccggc gcagccggct ttgctttttg
      181 tgcccagggc attctcagtt cgccatggcg ctgcgcgcca cccgcagcgt atgaaatcgt
      241 gcggtcggtg cgggggccac cgcggaaccg gctttgccgg gccgctggtg gaccttgcag
      301 gccgcatggc cgcgagacgc ggccattcct cgccctgtcc aaaccgacgc aggtcggttt
      361 ggagccgcag gtctcggccc cctcgagggg gagagggcga agcccgaggg ggcatcgccg
      421 aaggcgcttc gggggtgggc tttcacctca gcgcacgacc tgcgccaact cgcccttggc
      481 gtaccggctg gcgaccactt gcaggctctg gcccttgatc ttggcggctt ggccttcgca
      541 gccgaactgg atatagcgct gcttgcagat ctgcttggcg gcctcgcgcg ccggcttgag
      601 ccattcgcgc gcgtcgaact tctcggggtt ctcggccagg aacttgcgca ccgcgccggt
      661 catcgccagc cggatgtcgg tgtcgatgtt gatcttgcgc accccatgct tgatggcctc
      721 ctggacttcc tccaccggca cgccataggt ctccttcatg ttcccgccgt actgacggat
      781 cacggccagg agatcctgcg gcaccgccga cgaaccatgc atcaccaggt gcgtattggg
      841 aatgcgcttg tggatggcct tgacgcgatc gatcgcgagg atgtcgccgg tgggcttgcg
      901 ggtgaacttg taggctccgt ggctggtgcc gatggcgatg gccagcgcgt ccagctgcgt
      961 cttcttgacg aaatcggcgg cctgctcggg gtcggtcagc agctgttcac gcgtcatggt
     1021 ggactcggtg ccgtggccgt cctccttgtc ccccttcatc gtttcaaggc tgccgaggca
     1081 gcccagttcg ccttcgacgg tcacgccggc cttgtgcgcc atgtccacca ccttgcgcgt
     1141 cacgtccacg ttgtagtcgt agctggcgat ggtcttgccg tcggcctgca gcgacccgtc
     1201 catcatcacc gatgaaaagc ccaggttgat cgcgccctgg cagacgtcgg ggctctggcc
     1261 atggtcctgg tgcatgacga gcggaatcgc cggataggct tcgatggccg ccaggatcag
     1321 gtgcttgatg aaggactcgc ctgcgtactt gcgcgcgccg gcgctggcct gcaggatgac
     1381 cggggcgccg gtctccttgg ctgcctccat cacggcctgg acctgctcga gattgttgac
     1441 gttgaaggcc gggatgccat agccgttttc ggcggcatgg tccagcagtt cgcgcatgga
     1501 aacgagagac atgatgaggg tccgatctat tgagatgagg ttgcgcgcgg cagcgtgtaa
     1561 ccgcgccgta actgcgctcg attttagccg ctgcctcgcg cgcttccagc gtatcgcgcc
     1621 gacgagccca ctcgcgccgt cgcggacctg aaggtcacac acacacctct gcaacagcgt
     1681 gctgcctgcg ctatcccact gctcgggcta gctcgcgcgg cagatcttga gcatgttggt
     1741 gccgcccggc gcgcccatgg gctcgccgca ggtgatggcg tagatgtcgc cgctggccac
     1801 gatgccgcgt ttcttcaggt gcgcctcggc ctcgcccagg gccgtgtcgc gctccgcgct
     1861 ctggtccagc agcagcgggc gcacgttgcg gtacagggcc attttgcgct gggtcaccag
     1921 tttcggcgtc agggcgtaga tcggaatgtg aatgcggtgc cggctcatcc acagcggcgt
     1981 cgagcccgag tcggtcaacg ccacgatcgc cttgcagccc aggtgatgcg ccgtgaacag
     2041 cgcgcccatg gcgatcgact ggtcgatacg cccgaacgtc atgccggtga aatcggcgtc
     2101 gagcttcacg tcctcggcct tttcggcttc caggcagata ttggccatct cgacgatggt
     2161 ttccagtggg aagcggccgg ttgcggtttc ggcgctcagc atcacggcat cggtgccatc
     2221 gagcaccgca ttggccacgt cgctgacctc ggcccgcgtg gggaccggat tgttgatcat
     2281 cgactccatc atctgggtgg cggtgatcac caccttgtcg tgctcgcggg ccatgcggat
     2341 catgcgtttt tgcagcgccg gcacggcggc gttaccgacc tcgatggcca gatcgccgcg
     2401 tgccaccatg atcgcgtcgc tggccttgag gatggattcg aggttgggaa tcgcctcggc
     2461 ccgctcgatc ttggcaacca gccccggcct gtggcgaaac tccgcgccgg ccacgttgca
     2521 aagctggcgc gccatctcca tgtcggtcgc gttcttcggg aaactcaccg ccacgtagtc
     2581 ggcctggaaa ttcatcgcgg tgcggatgtc ctccatgtcc ttggccgtca gcgccggggc
     2641 cgtgaggccg ccacccttct tgttgatgcc cttgttgttg gacaactcgc cgccttgctt
     2701 gacggtggtg cgcacctgct cgccgcgcac cgcgtccacg gtgagcacga tcaggccgtc
     2761 gttgagcagc agcaggtcgc ccgctttcac gtcgcgcggc agctccttgt agtccaggcc
     2821 cacgccgttg gcgtcgcccg gttccgagcg ggaggcatcg agcacgaact tcatgcccgg
     2881 ctccagcgcc accttgccgt cggcaaactt gccgacgcgg atcttggggc cttgcaggtc
     2941 ggccatgatc gccacttcac gacccgcgcg ctgcgaggcc gcccgcacca gggccgcgcg
     3001 atcgatgtgg tcctgcgcgg tgccatggct gaaattcagc cgcaccacgt tcacgccggc
     3061 gcggatcatc tgatcgagca gcgcgggatc gctggaggcg gggccgaggg tggcaacaat
     3121 cttcgtggcg cgacgggcca tgagttctcc tgtaactaag tttccaattt tcccatggaa
     3181 gcagttacat gccggttacc aaaggcgtgc gttgcgagaa atcagccgga accggaactc
     3241 tacgcgcgtg aagccaaggt tcgcgcgccc tcgaaaacgg gggtgctgga tagggaaatc
     3301 ttcctatgcg aatccactct tcttgcaatt tccatcgcgg agcgcccgac ctaagctgag
     3361 cccgaaacga aaacggagct ttgcaatgag aactcaaccc gagcccacgc ggcgcgcctt
     3421 cctggccgcg ctgccggccg tcaccgcggg gctggcgtct cccgccgccg cttctcagga
     3481 agaagtcccg cgctggggca tgctgatcga tacgcggcgc tgtatcggct gccaggcctg
     3541 cacgattgcc tgctcgatgg agaacgtatt gcccgagggg caattccgca cttcagccgc
     3601 gacctatgcg gtgacgaacg gcaagggaca gaccggcatg tcgatgctgc cgcgcctttg
     3661 caatcactgc gaagagccgc catgcatccc ggtctgcccc gtgggcgcca ctttcaaggg
     3721 caaggatggc gtcgtcctgg tggatgcgga tcgctgtgtc ggctgcggct actgcgtgca
     3781 ggcctgccac tacgaagccc gctttatcaa tcacgacacc ggcaaagccg acaagtgcac
     3841 cttctgcagc caccgggtcg aagccggcct gttgccggcg tgcgtcgaga cctgtgtcgg
     3901 cggcgcgcgc gtttttggtg acctgaacga tcccgccagc acgatcagcc gtcgcatgca
     3961 ggcggcccag ggcaagacca gcgtgctgaa gccggaagcc gggactcggc ccagcgtgtt
     4021 ctacatcggg ctggacaagg aactgaacgg ccgggtcacg ggtgcctcgg cgaccgaaat
     4081 gatgtggcgc ccgaaccaac agggagcatg aacatgaaca ccactgtcga aatactcggc
     4141 tttacccgcg aacccggctg gctgccgtgg gccgtgcagt attttttcct gatcggaatt
     4201 tccgccgcgg ccttcttcat ctcgctgcct ggcctggcct ggcgacatcc cgagtggcgt
     4261 ggcgtctccc ggcgtgcgct gctggccgcg ctcgtgtgcg gactcactgc cccggttgcg
     4321 ctgctggccg acctgcatca gccggggcgg ttcctcaact tctacctgca tccgaacctg
     4381 cagtcctgga tggcctgggg atcgtttttc attccgctgt acctgttcgg actgctgctc
     4441 tacgcgtggt tgtgcctgcg gccgcagctg gccggcctgg cccgggaaca cgaggccggt
     4501 acgcgcctcg cgaagctcta tcgcgcgctc gcctacggcg gccacgacaa ccggggcgcg
     4561 atccgcgttg cggccgtgct cgcggcgctg ggcgctgtcc tggtcctgct ctacaccggc
     4621 atggaggtga tggtggtgcg ggcccggccg ctgtggaaca cgccgctgct gccgccgctg
     4681 ttcgcagtga ccgcgtttgc gggcgggctt ggcatgacgg cgctgttcga ggcgctgtct
     4741 ggccaccgcg attcggcgcc cctgctcaat cgctgggcgg cgcgcagcca atgggcggtg
     4801 ctggccctgc tcgccgcctg gctgctttcc gcattcactg gaatcgcgcc agccgcggcc
     4861 gaggcactgg catcgctacg aggctccgtg agctggcgcg tcacgctggg gtccctggtg
     4921 gccgtctcgg tgctgacgct ctgggtcgca cgcaagcggc gcgattcgct ggtcctgatg
     4981 gcactgctcg cgctgctcgg tgcctggctg ctgcgctggg ctctcatcat gggcgggcag
     5041 ggtatcccca agatgggcgc gaccttccgc agctactacc cgacgctgac gccggacagc
     5101 gcgctgggca tcgtcggcac cgccggtctc ttcctcaccc tctacatcgt cctgaccagc
     5161 ttcgtcccat gggacgaatc cacccaagcc gcgggagccg catcatgaaa atcagcagac
     5221 gccgccaggt cctggcgctc ggtggcatcg ccgccttcgg ggccggtttt tcggaaaccg
     5281 ctggccgcat ggcaggcaag ctcatgggcc gcgacaaacc cacgcacaag acggccggca
     5341 atgcgcccac cccggaattc aaggtggaca gccagggcaa cgtcgccatc aacaccgccc
     5401 agcaggtcag ttacaccacc tgcctcgggt gcaccacgca atgcggcgtg cgcgttcgca
     5461 ccgatcgtgc gacgggcaag gtgctgcgcg tcgcgggcaa tccgtacagc ccgatgtcca
     5521 cggatccgca cctgccgatg aaagcgagcg tgcgcgagag ttttgccgcg atctccgcat
     5581 tcgagggcaa ggggctcgcg ggccgctcca cggcctgtgg ccggggcaac gcggtggcgc
     5641 agcagatcga ttccccgtat cgcgtgctca cgcccttgaa gcgggtgggc gcgcgcaaca
     5701 gcggccgctg ggaaccgatc tccttcgagc aactggtcaa ggaagtggtc catggcggca
     5761 acctgttcgg tgaaggcccg gtcgagggac ttgccgcctt gcgcgcattg gaccagccga
     5821 tcgacagcaa taagccggaa ctcgggtcgc gcgtcaacca ggtagcggtg ctgtccagcg
     5881 tcaacgacgg gcgcgagagc ttcgcgcgcc gcttcttcca gaagtcatac ggcactctca
     5941 acttccttgg acatggctcc tactgcggcg gcgcttaccg ctcgggcacg ggcgccctgt
     6001 tcggcgacct gaaagcaatg ccgcacggca agccggattt cgccaacgcc gagttcgtgc
     6061 tgttcgtcgg caccgcgccc gggcaggccg gcaatccttt caagcgccag ggcaccctcg
     6121 tcgccaaggc gcggaccgaa ggaaagctgt cttatgtcgt ggtcgatccg gtgctgaccc
     6181 acgccagcag ccgcgccagc ggcgaccgcg ggcggtggct gccgatccgt ccgggtaccg
     6241 atggcgccat ggcaatggcc atcatccgct ggctgatcga gaacgagcgc atcgacagcc
     6301 gtttcctcgg ctttccgaat gctgcgacgg cgagggaaaa cggagagccc tcgttctcca
     6361 acgccacgca cctggtggtg gtggagccca agcatccccg tgagggaagg atgctgcgcg
     6421 cgtcggacct cggcgccgcg atgaccgaag agcagcgcta caaggaagcg gacgccttcg
     6481 tctgcctcga tgcggcgggc aaggccgtgc ttcacgacaa ggcgaagggg ccggcccagc
     6541 tcttcgtgga cacggtgctg caagtggaag gcaaggacgt gcgcgtcaag tcctccctgc
     6601 aattgctcaa ggaagaagcg atgcggctgg acctgagtgc gtatgccgcc gcctgcggta
     6661 tcgaagcagc aacgctgacc gcgctggcaa gcgagcttgc gtcgcacggc aagcgcgctt
     6721 cggtgatcgc ccacggcggc atgatgagcg gcagcggctt ctacaacgcg tatgccctca
     6781 tgagcatcaa tgcgctgctc ggcaacatca actggaaggg tgggatggtt gcgaacggcg
     6841 gcggcttcaa gccggatggc gaaggccccc gctacaacct cgacacgttc gccggcatgg
     6901 tcaagccggg cggcacgcca ctcgggcgca atgtccccta cgaaaagacg tcggagttcg
     6961 cggcgcgcaa ggccgacggc aagccgtacc cggcgaaggc gccctggttc ccgaacgcgc
     7021 cggggctgac caccgaactg ctgcccggtg gcatcagcgg ctatccgtat tcactgaagg
     7081 cgctgatcct gtggtcgtcc aacccgatgt acggcatccc gggtctgcgc gagcgcatcg
     7141 ccaaggacct ggcggacccg gcgaagctgc ccttgatcgt ctcgatcgat cctttcatca
     7201 acgagagcaa cgcgtacgcc gactacatcg tgccggactc gctgatgtac gaaagctggg
     7261 gctgggtggc cgcgtggaat ggcgtgccaa ccaaggcgat gggagcgcgc tggccggtca
     7321 tggaaccgaa agcacaaaag acgccggaag gccgcgccat cggcatggaa accttcttca
     7381 tcgctttggc cagggccatg agtcttcccg ggttcggcag cggcgccatc accgacaccg
     7441 acggcaatga ctacgcgctc gacggcccgg aagactggta cctgcgcgcg ggtgccaaca
     7501 tcgcatggct gggccaggag ccggtgggca atgcgagtga agaagacatc ctcctgtcgg
     7561 gagtgcaccg gctgcgatcg gtgctcgaaa gaacgctgaa agccgaagag gtggccaagg
     7621 tcgcgttcct gctcagccgc ggcggccgct accagccggc caaggacgca tacgacgaag
     7681 agaacgccga atggatgcgc aatcagctca agcccatgtg ccacctgtgg aacgagaacg
     7741 tgggcggctc gaaaaacagc ttgtccggca agcgcaatcg cggttgcgcg acctggctgg
     7801 agcccgcgtt cgccgacgga accccgatgc gcaaggtcta ccccgaaaag caatggccgt
     7861 tgcaactggt gagctacaaa tcggcgttgc agaatccgta ctcgatcggc gccacgcgcc
     7921 tgttggcggt gcatccggaa aacccggtcc tgatccatcc cgacgacgcg cagcgcctgg
     7981 ggctggcgat gggggacctg gctgaaatca cgacgcccgg tggcagcctc aaggcgcgcg
     8041 tgatggtaca cgcgggcatc gcagccggcg tcgccgcctt cgagcacggt ttcggccatc
     8101 gggaactcgg cgcacgggcg caccgcatcg gcggcaagca tcagcccgag gaccggcggc
     8161 tggctgctgg cgtcaacctg aacgacatcg gcctgatgga tcccatgcgg cccgaccggg
     8221 cgccgtgggt ggacgccatt tccggcagct cggtgcgcaa cggcctgccc gcgaaaatca
     8281 ggccggcctg atcgcgtcgt ccagcgccgc cgggtcgact cgcagcatca ggcgggccaa
     8341 ctcggcggcc gaggacaccc gcgccttttc catgacatgc tggcggtgga tgtgcaccgt
     8401 tttttcgctg atgccaagag ccgcggcgat gagcttgttg ggttgcccct gggccaccag
     8461 gcgcgcgacc tcgcgttccc gcggcgacaa gccggcgagc agggcgatcg cctcgtcgcg
     8521 aacctgcctt tcctgcagcg attcggcgca gcggcgcatc gcggccgcaa cggcgtcgag
     8581 cagcacctgg tccttgaacg gcttttcgat gaagtcggcg gcgcccgatt tcatggcctg
     8641 cacggccatc gacacgtcgg cgtggccggt catgaaaaga atcggaatat cgatgccacg
     8701 ctggcgcagc acctgctgca actcgagtcc gctcatcgca ggcatacgga tgtccagcag
     8761 cacgcaggtc ggctgcgttg ggttggcgac ggcctccagg aactcctcgg cggaggcgtg
     8821 caattgcact ttccagtcca ccgactccag cagaaaacgc agcgaccggc gcaggcccgc
     8881 atcgtcgtcc acgacttgga caatgcactg gtcaggctgg taattcattg cagggaagag
     8941 aaaaagaaaa acgcatgcct tgcccgtcag gattgggctc ggcccacagg cggcctccat
     9001 gcgcttcgat gatagtcttg cagatcggca ggcccagccc cagaccatcc ggcttggtcg
     9061 tgaagaaggg ctggaacagg cgcgctcgct gggcggcatc gagacccact ccgtgatcga
     9121 gtacgtgcac caccaggttg ctgccggccg cttcgatggc aaccttgatg ttccggcgtt
     9181 ccgcggggag gtcgcggctt gaatcgatcg cgttcttgat caggttgagc aggacctgct
     9241 ggatctgcgg gccgtccgcg agcacccggc atacctgctg gcgccggtct tcgacggcga
     9301 tatcgggcgc gcgcgccagc atgccggaga tcaatcgcct tgcctcttgt gcgatcgacg
     9361 caaggtcgac cggatcgcgg gccgcgacgc gcttcctcgc gaaatgccgg atccgttgca
     9421 cgatgccgcc ggcgcgctcg gcttcgctgg cgatctcggt gcaggcctcc gtcacggctt
     9481 cgggggtcaa ggtgccgccg gactgccttc tgagcacact gcgggcgtag gtgccgatgg
     9541 tggtcagcgg ctggttgatt tcgtgggcca gattgcctga aagctcaccc agcacggaca
     9601 gccgcgacag gtgctccatc tgctcctggc tctcggtgat gcgcatttcg gcttgttgtc
     9661 gtgcctgcat ggattcctgc aactgggcgg cgcggcggtg caccaggtac tcgacccgca
     9721 cggtgtggac gacccaggcc accagcagca ccagcacgcc cgccagccac ggccagaacc
     9781 gtttggccag gcccgcgggc gtcacgtcgc gcagcgaggc ataggggcca acgcgcagtt
     9841 cgcgatagag ctcgtgcacc ggctggtaat cggccggcac cgcccagctc atgcttccct
     9901 cgcgttcggg catggccagc agcgcccgcg ccacggtttt cgccagagcg ggatcggtct
     9961 ggcgcgtgac cgcaatgggc cagtcggggt agagctgcgt ggacagcgca caggcaaacc
    10021 catcgattct cgtgggcgac aggatcctga agctcgccag cgagagttca ccccggcctg
    10081 ccatctgttc gagcacgcag acgcgaacga tgccggcatc cacctcgccg cgctgcaccg
    10141 cttcgaccac gcgcgccatc ggcaggccga cgaaacgcag ctcctccagg tctgactcgg
    10201 tatcgacgcc ctggcgcagc atttcgcgcg cggcgacgag gtacccaccg aaggcctccg
    10261 gagcgacggc agcgactctt ttcccggcga gagcggacag ctcgcgcagt tccgcgcggt
    10321 cggcccgtgc gaataccgcg gatccgatcg cgcgatcggg agttccatga ggcgatgaca
    10381 gcgtggcgat ccggctgagg ccgaactcgc cctccatcgt gatgtagtag cccggattgg
    10441 tgacgaccag atcgacccga tgctcggcga ttgcttttcg caggcccgcc gcatcgtggt
    10501 gatcgagccg gaaacggtgc tccggcaatg cagcgttcag ataatcgagt accgccgacc
    10561 agtcctcgct ggcggcgcgt tcgccctgat aagcgaagac gccgattgaa acatcccggg
    10621 ccggcgcctc attggcccaa aaggcgagtg ccgccgcgaa caggagtctg gtgatcgaga
    10681 acatgccacc ccgactattc gcattgcggc gccagcgcag cttgaccagg atcaagcagt
    10741 cgggctttgc aggagcgcgc cggctcaggg gtatgctgcc gggtcaagcc cggcatgaca
    10801 aattcgccat ggcagacagc tccttagccg ttagcgcgct tttccaggat ctcgaacgcc
    10861 ggcagcgtct tgccttccag cacttcgagg aaggcgccgc cgccggtgga gatgtagcct
    10921 acgttcttct cgatcccgta cttggcgatc gccgccagcg tatcgccccc gccagcgatg
    10981 ctgaaagcgg gcgattgcgc gatggcgcgg gcgatggttt cggtgccgtt ggcgaaagcg
    11041 tcgaactcga ataccccgac cggcccgttc cagacgatgg tgccggccga cttgagctgc
    11101 tcggcgagct tgcgcgcggt ctgcggcccg atgtccagga tcaggtcgtc gtcggcgacg
    11161 tcggtggcag ccttgacttg cgccggcgca tcggccgcaa aggctttggc ggtgaccacg
    11221 tcggtgggaa tcggcacggc agcgccgcgc gccttcatcg cctcgatcac cgcccgggct
    11281 tcggacacga gatcgacctc ggccagggac ttgccgatct tcaggccggc ggcgagcatg
    11341 aaggtgttgg ca
//
LOCUS       DASWIY010000188        10367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_479729, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000188 DASWIY010000000
VERSION     DASWIY010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479729"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VGA59_06965"
     CDS             complement(<1..820)
                     /locus_tag="VGA59_06965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HEX9719458.1"
                     /translation="MFKALVLDKSPEFSACVREVDDSFLPEGDVTIDVAYSTLNYKDG
                     LAITNRSPVVRSWPMVAGIDGTGTVVSSSSPLWKPGDQVILNGFGVGETHKGCLAGRA
                     RLKGEWLIRRPPRFSARDAMAIGTAGYTAMLCVLALERHGVKPGDGEVLVTGATGGVG
                     STAIALLSKLGHRVVAATGKASETDYLKSLGAGSVIDRSELAAPGKPLQKERWAGVVD
                     PVGSHTLVNACAQTLYGGVVAACGLAQGLDFPATVAPFILRAVTLAGVDSVMAPL"
     gene            1031..2788
                     /locus_tag="VGA59_06970"
     CDS             1031..2788
                     /locus_tag="VGA59_06970"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX9719459.1"
                     /translation="MSETPFDPPETGGDEPAGMRKGLTSYGDAGFSLFLRKAFIKGAG
                     YTDAALDRPVIGIADTGSGYNPCHGNMPQLVEAVQRGVLLAGGLPVKFPTISVAESFS
                     HPTSMYLRNLMAMDTEEMIRAQPMDAVVLIGGCDKTVPAQLMGAASAGIPAIQLITGA
                     MLTGSHRGERVGACTDCRRYWGRFRAGEIDNEEIAAVNDQLVASVGTCSVMGTASTMA
                     CIAEALGITVPGGASPPAVTADRMRIAELTGAQAVEMARTGLTIDKVLTAKAFENAMR
                     VLLAIGGSTNGIVHLTAIAGRLGFEVDLKALDRMGRETPVLVDLKPSGRHYMEDFHAA
                     GGMTTLLCELKPLLHLDALTVTGRTLGEELERAGPPFAQDVVRPRANPIYPQGGIAVL
                     EGNLAPGGAIIKQSAADPKLMEHEGRAVVFENMADLVQRIDSDNLDVRSDDVLVLKNI
                     GPKGAPGMPEAGYIPIPRKLARAGVKDMVRISDGRMSGTAFGTIVLHVTPESAVGGPL
                     AHVRSGDRIRLSVSRREISLLVSDEELERRAKSNPVTPPSAERGYRKLFLDSVTQADK
                     GVDFDFLRPAQTRGRVPEA"
     gene            complement(2864..4141)
                     /locus_tag="VGA59_06975"
     CDS             complement(2864..4141)
                     /locus_tag="VGA59_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9719460.1"
                     /translation="MTIAALFILLFVLMGLGLPIAVALGLSSLVTILMFARDSLASLS
                     LKLFETSEHYTLLAIPFFILGGSFMTTGGVARRMIRFANACIGHMRGGLAMASVMACM
                     LFAAVSGSSPATVVAVGSIVIAGMVKAGYPQTFAAGVICNAGTLGILIPPSIVMVVYG
                     AATETSVGKLFMAGVVPGIVLGLMLMVAIFVRARMIDLPRQPRASLRDVLISGRDSLW
                     GLLLIVIILGGIYGGIFTPTEAAAVAAVYAFLIAVFVYRDITLSKVPHVLLESGKVTI
                     MLMFIVANALLFAHVLTTERIPQTIAETIIAWGMPAWGFLIVVNILLLVAGMFMEPTG
                     IILILAPILFPIAVRLGIDPVHLGIIMVVNLEIGMVTPPVGLNLFVTAGITRMSIMAV
                     AHAALPWTLILLTFLILITYVPSITLLLPNLLF"
     gene            complement(4138..4671)
                     /locus_tag="VGA59_06980"
     CDS             complement(4138..4671)
                     /locus_tag="VGA59_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9719461.1"
                     /translation="MRWLEYLEETLVMLLMAAMTLITFMQVVARYVFNYSFVWALELT
                     GVLFAWLIFVGMSYGVRVGAHIGVDLAVRSLGARGARIVGALAAVLCIVYALIVTWGG
                     YQYVSKMYDVGILMQDMPVPQWIPRLVLPLGFALLAFRFAQVLWRIARGQEVHMLVDE
                     AKDALKLKEDDLDKVQK"
     gene            complement(4714..5727)
                     /locus_tag="VGA59_06985"
     CDS             complement(4714..5727)
                     /locus_tag="VGA59_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9719462.1"
                     /translation="MTINHPSRRNVLAAGAAVAGAVAMPFMARAQAPIVIKFSHVVAE
                     QTPKGLGALKFKEVAEKKLPGKVNVQVFPSSQLFGDAKELEALLLGDVQLIAPSLSKF
                     DRYTKKIQVFDLPFLFDDIQAVDAFQSGPQGKALLESIVSRGLRGLGYWHNGMKQLST
                     NKDKLTRPEDVKGLKFRIQASDVLEAQFRALGANPQKMAFSEVYQALQTGVVDGQENT
                     WSNIYSQKFHEVQKTIAETNHGVIDYMVVTNAKWWDGLAPDIRKGLSEAMAEATAHAN
                     KLASDINETDRKKIADAGKAKIQKLSKEDVAAWRKAMEPVWKKFEGDIGADLINAALK
                     SNK"
     gene            complement(5951..7318)
                     /locus_tag="VGA59_06990"
     CDS             complement(5951..7318)
                     /locus_tag="VGA59_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX9719463.1"
                     /translation="MHTRTTELAAFAAGLKFDAIPAAVLRKAEDLLVDWFGSAIAGKD
                     ARPVETIAGFACEMGPVSKAGQGGSELLVRRTFTSPYFAAMANAAASHVAEQDDVHNG
                     SVFHPATVVFPVALALAQARGASGKQLLTAAVAGYEVGIRVGEFLGRSHYKVFHTTGT
                     AGTLAAAACAGNLLGLDAAQMQHAFGSAGTQSAGLWEFLRTAADSKQLHTAHAAAAGL
                     MAACLAQEGFTGAAEILEGAQGMAAGMSTDADPSRLDDGLGDRWATLETSFKFHASCR
                     HTHPAADALLQVMQAHALAPEDLDRVVTHVHQGAIDVLGPVTRPATVHQSKFSMGTVL
                     ALVARFRQAGLNEFEAHFLDESTQALRDRVTMELDAEVDAAYPRRWIGKVTVHTTDGR
                     VLKGRVDEPKGDPGNTLSREEITAKALRLAAFSGAASPAEMKAAVDRLWQVATWPRVG
                     PLLAA"
     gene            complement(7377..8567)
                     /locus_tag="VGA59_06995"
     CDS             complement(7377..8567)
                     /locus_tag="VGA59_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9719464.1"
                     /translation="MTTAVRPLDGITVVSLEHAIAAPFCTRQLADLGARVIKVERPGG
                     GDFARDYDARVRGLASHFVWTNRSKESLTLDLKQAGALAVLKRLLAGADVLVQNLAPG
                     AAARMGLGYEALSREHPRLIVCDISGYGEDGPYRDKKAYDLLIQSEAGFLSVTGTPES
                     PSKAGNSVADIAAGMYAYTGILSALMLRAKTGEGSHIDVSMLESLAEWMSYPMYYAFE
                     GAPPPPRTGASHASIYPYGPFDAGDGGTVMLGLQNEREWKVFCELVLRDVALAADPRF
                     DSNARRNEHRNELTAIIVQAFSALTTQQVVARLDDAQIANARMNDMAGLWAHPQLAAR
                     ERWRRIGSPVGEIPAMLPPGRFNSFDYRMDPVPAVGQHTEAILRELGHGDAEIAALQQ
                     SRAI"
     gene            complement(8578..9741)
                     /locus_tag="VGA59_07000"
     CDS             complement(8578..9741)
                     /locus_tag="VGA59_07000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9719465.1"
                     /translation="MLKTTPDQYQEIRDAVRALCAEFPGEYHRQIDEKRGYPEAFVDA
                     LTKAGWLAALIPADYGGSGLGLAEASVIMEEINRSGGNAGACHGQMYNMGTLLRHGSQ
                     AQKDLYLPKIASGEWRLQAMGVTEPTTGTDTTKIKTMAVKKGDRYVINGQKVWISRVQ
                     HSDWMILLARTTPLAEVKKKTEGMSIFMVDLRKAEKAGLTVRPIPNMVNHETNELFFE
                     NLEIPAENLIGQEGMGFKYILDGLNAERTLIAAECIGDGYWFIDRVSKYVSERVVFGR
                     PIGQNQGVQFPIAESYIEVEAANLMRFKACQLFDAHQPCGAEANMAKYLAAKASWEAA
                     NACLQFHGGFGFANEYDVERKFRETRLYQVAPISTNLILSYVAEHLLGLPRSF"
     gene            complement(9785..>10367)
                     /locus_tag="VGA59_07005"
     CDS             complement(9785..>10367)
                     /locus_tag="VGA59_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9719466.1"
                     /translation="WARDNPLTVGDAIQRISRIESVTHKAGRSGDLLFVLVKHEVHNA
                     QGLALTEEHDIVYRAAAQPGDPQPTPVAAEQGAAWQREIVPDDVLLFRYSALTFNGHR
                     IHYDRRYVTEVEGYPGLVVHGPLIATLLADLVRRHAPGAFMQRFEFKAVRPSFDPHPL
                     RVNAQPSADGKTVRLWAQDHEGWLTMQATADLA"
BASE COUNT         1844 a         3373 c         3371 g         1779 t
ORIGIN      
        1 ccaatggggc catgacgctg tcgacaccgg cgagcgtcac cgcgcgcagg atgaagggcg
       61 ccaccgtcgc gggaaagtcg agcccctggg ccaggccgca agcggccacc acgccgccgt
      121 agagtgtctg ggcgcaggca ttgaccagcg tgtggctgcc caccgggtcg accacgcccg
      181 cccagcgctc tttttgcagc ggcttgccgg gcgcggcaag ttcggagcgg tcgatcaccg
      241 agcccgcgcc cagcgatttc aggtagtccg tttcagaggc tttgccggtt gcggccacga
      301 cgcgatgacc cagcttggac agcagcgcga tcgcggtcga tccaaccccg ccggtcgcgc
      361 cggtcaccag cacttcgccg tcacccggtt tcaccccgtg ccgctccagc gccagcacgc
      421 acagcatggc ggtgtagccg gcagtgccga tcgccatcgc atcgcgagca ctgaagcgcg
      481 gcggtcgacg gatcagccat tcgcccttga gccgcgcgcg cccggcgagg caacccttgt
      541 gcgtttcgcc gacgccgaag ccgttgagaa tgacttggtc gccgggtttc cacagcggcg
      601 aggaactgga cacgacggta cccgtgccgt cgatgcccgc caccatcggc cagctgcgca
      661 ccacgggcga gcggttcgtg atggccaggc cgtccttgta gttgagcgtc gaataggcca
      721 cgtcgatcgt gacgtcaccc tcgggcagga acgagtcgtc cacctcgcga acgcaagcgc
      781 tgaattcggg ggatttatcg aggaccaggg ctttgaacat ggtgctttcc ttgggggaga
      841 acctgagcat aacgaaaacc cgaaccggcc acccggatgc ccgccacagc agtgtgaggg
      901 agtgattggc gcaaaatcgc agcagacgac tggatacacc aacttggagt tctacaaggc
      961 aagcgacgtg tgggtggtcg tcagaagccc cgaccaactc gtcttttgtt tttcgcacga
     1021 aggaactccg atgtccgaaa ctccattcga tcctcccgag accggcggcg acgaaccggc
     1081 cggcatgcgc aaggggctga cgagttacgg cgacgcgggt ttttcgctgt tcctgcgcaa
     1141 agccttcatc aagggcgccg gctacacgga cgccgcgctc gaccgcccgg tgatcggcat
     1201 cgccgacacg ggcagcggct acaacccctg ccacggcaac atgccgcaac tggtggaggc
     1261 cgtccagcgc ggcgtgttgc tggccggcgg gctgccggtg aagttcccga ccatctcggt
     1321 ggccgagagc ttctcgcacc ccaccagcat gtacctgcgc aacctgatgg cgatggacac
     1381 cgaggagatg atccgcgcgc agccgatgga tgcggtggtg ctgatcggcg gctgcgacaa
     1441 gacggtgccg gcgcagctga tgggcgccgc ctcggccggc atccccgcga tccagctgat
     1501 caccggggcc atgctgacgg gctcgcaccg gggcgagcga gtcggcgcct gcaccgactg
     1561 ccgccgctac tggggacgct ttcgcgcagg cgaaatcgac aacgaagaga tcgccgctgt
     1621 caacgaccag ctggtggcca gcgtcggcac ctgttccgtc atgggaacgg ccagcaccat
     1681 ggcctgcatc gccgaggcgc tgggcatcac cgttccgggc ggcgcatcgc cgccggccgt
     1741 gacagccgac cgcatgcgca tcgccgagct gaccggcgcc caggcggtcg agatggcgcg
     1801 caccgggctc accatcgaca aggtgctcac cgccaaggcg ttcgagaatg cgatgcgcgt
     1861 gctgctcgcg atcggcggct cgaccaacgg catcgtgcac ctcacggcga tcgcgggccg
     1921 cttgggtttc gaggtcgacc tgaaagcgct cgaccgcatg ggccgcgaga cgccggtcct
     1981 ggtcgacctc aagccctccg gccggcacta catggaggat ttccacgccg cgggcggcat
     2041 gacgacgctg ctgtgtgagt tgaaacccct gctccacctg gatgcgctca ccgtcactgg
     2101 ccgcacgctc ggcgaagagc tggaacgcgc cggcccgccg ttcgcgcagg acgtggtgcg
     2161 gccgcgcgcc aacccgatct atccgcaagg cggcatcgcg gtgctggagg gcaacctcgc
     2221 ccccggcggc gccatcatca agcagtccgc cgccgacccc aagctcatgg agcacgaggg
     2281 ccgggccgtc gttttcgaga acatggccga cctggtccag cgcatcgaca gcgacaacct
     2341 cgacgtgcgt tcggatgacg tgctcgtgct caagaacatc gggcccaagg gcgcgcccgg
     2401 catgcccgag gccggctaca tcccgattcc gcgcaagctt gcgcgagcgg gcgtgaagga
     2461 catggtgcgc atttcagatg gccgcatgag cggcaccgcc ttcggcacca tcgtgctgca
     2521 cgtgacgccg gaatcggcgg tggggggccc gctggcccac gtgcgcagcg gcgatcgcat
     2581 ccgcctgtcc gtatcgcggc gtgagatcag cttgctggtc tccgacgagg agttggagcg
     2641 gcgcgcgaag tccaaccccg tcacgccgcc atccgccgaa cgcggctacc gcaagctgtt
     2701 cctcgacagt gtgacgcagg ccgacaaggg cgtggacttc gatttcctgc gcccggcgca
     2761 gaccagaggg cgcgtgcccg aagcctgacg cacgggccgc cggttgggcg acggtcccgc
     2821 gaacaatacc gaacgaaatc gacgaaatcg cttcgcgcgt gcgtcagaac agcaggttcg
     2881 gcagcagcaa tgtgatcgac gggacgtacg tgataagtat gaggaaggtc agcaggatca
     2941 atgtccatgg caaggccgca tgggccacgg ccatgatcga cattcgggta atccccgccg
     3001 tgacgaagag gttcagaccg accggcggcg tcaccatgcc gatctccagg ttcaccacca
     3061 tgatgatgcc caggtgaacg gggtcgatcc ccagcctgac tgcgatcgga aaaaggatag
     3121 gggccaggat caggatgatg ccggtgggct ccatgaacat gccggcaacc agcagcagga
     3181 tattcaccac gatcaggaac ccccaggcgg gcatgcccca ggcgatgatg gtttcggcga
     3241 tggtctgcgg aatgcgctcg gtcgtcagca catgcgcgaa caacagggcg ttggccacga
     3301 tgaacatgag catgatcgtg accttgccgg attcgagcag cacatgcggt accttgctca
     3361 acgtgatgtc gcggtacacg aacacggcaa tcaggaaggc atacacggcc gctaccgccg
     3421 ccgcctccgt gggcgtgaag atgccgccgt agatcccgcc caggatgatg acgatcagca
     3481 acaggcccca aagcgaatcc cgccccgaga tgagcacgtc ccgaagggag gcgcgcggct
     3541 gccgcggcag gtcgatcatg cgggcgcgca cgaaaatggc gaccatcagc atcagtccga
     3601 gcacaatgcc cggcacgacc ccggccatga aaagcttgcc caccgacgtt tcggtggctg
     3661 caccgtacac caccatcacg atggacggcg gaatcaggat gcccagggtt ccggcgttgc
     3721 atatcacgcc cgccgcgaaa gtctgcgggt agcctgcctt caccatgccg gcgatcacga
     3781 tcgaccccac cgccaccacg gtggctggag acgaacccga cacggcggca aacagcatgc
     3841 aggccatcac ggaggccatc gccaggccgc cgcgcatgtg gccgatgcac gcattggcaa
     3901 aacggatcat ccgccgggcc acgccacccg tggtcatgaa ggacccgcca agaatgaaga
     3961 aggggatcgc cagaagcgtg tagtgctcgg aagtttcgaa gagcttgagc gacagcgacg
     4021 ccagcgagtc ccgggcgaac ataaggattg tgaccagcga cgacaggccc agcgcgacgg
     4081 cgatcggcag gcccaggccc atcaggacga aaagcagaat aaacagtgcg gcaatggtca
     4141 tttctgcacc ttgtcgagat cgtcttcttt gagcttcagc gcgtccttgg cttcatcgac
     4201 cagcatatgg acctcctggc cccgggcaat tctccagagc acctgggcga accggaacgc
     4261 cagcagcgcg aaccccaggg gcaggaccag gcgcgggatc cactgcggaa caggcatgtc
     4321 ctgcatcagg atgcccacgt cgtacatctt gctcacatac tggtagccgc cccatgtgac
     4381 gatcagcgca tagacgatgc acagcacggc ggccagcgcg ccgacgatcc gggccccccg
     4441 ggcacccagc gacctgacgg ccaggtcgac cccgatatga gcaccgacgc gcacgccgta
     4501 cgacatgccg acaaagatga gccacgcaaa caggaccccg gtcagctcca gcgcccagac
     4561 aaaactgtag ttgaatacgt agcgcgcaac cacctgcatg aacgtgatca gggtcatggc
     4621 cgccatgagg agcatgacca aggtctcttc gagatattcg agccagcgca tggaagaaca
     4681 aagggcccgc aaagcgggcc cttcctgtgt tgcttacttg tttgacttca gtgccgcatt
     4741 gatcaggtcg gctccgatgt cgccttcgaa cttcttccag accggctcca tcgccttgcg
     4801 ccaggccgcc acgtcttcct tggacagttt ctggatcttg gccttgccgg cgtcggcaat
     4861 cttcttgcga tcggtttcgt tgatgtccga agcgagcttg ttggcatgcg ccgtcgcttc
     4921 ggccatggcc tcggacagcc ccttgcggat atccggcgcc agcccgtccc accacttggc
     4981 attggtgacg accatgtagt cgatgacgcc gtggttggtc tcggcaatcg tcttctgaac
     5041 ttcatggaat ttctgcgagt agatgttcga ccaggtgttt tcctgcccgt ccaccacgcc
     5101 ggtctgcagg gcctggtaca cctcggaaaa agccatcttc tgcggattgg cgccaagtgc
     5161 gcggaactgc gcttccagca cgtcggaagc ctggatgcgg aacttgaggc ccttgacatc
     5221 ctcggggcgg gtcagcttgt ccttgttggt ggacagctgc ttcatgccgt tatgccagta
     5281 gccgaggccc cgcaatccac gcgagacgat cgattcgagc agtgccttgc cctgcgggcc
     5341 ggactggaag gcatcgacgg cctggatgtc gtcgaacagg aaaggaagat cgaagacctg
     5401 gatcttcttg gtgtagcggt cgaacttgga cagggacggc gcgatgagct gcacgtcgcc
     5461 gagcagcagg gcctcgagtt ccttcgcatc gccgaacagc tgcgagctcg ggaacacctg
     5521 gacgttgact ttgccgggca gcttcttttc cgccacttcc ttgaacttca gggcgcccag
     5581 gcctttgggc gtctgctccg cgaccacgtg gctgaacttg atcacgatgg gcgcctgcgc
     5641 cctggccatg aatggcatgg ccaccgcgcc ggccaccgcc gcccccgctg ccagtacgtt
     5701 gcgccgtgat ggatgattga tcgtcatgtt atctccttgg tttcagaact cgtgactggg
     5761 aatccttgtc gcagcgtatg cattctcacg ctgccgtcaa gggcggtgct gctagcaccg
     5821 gtcggaaatg acgacttaat tctaaaatcg gatcagtccc cattttcgtg ggaactacgt
     5881 acgatttgtt tcacggtctc ttgagtttgg tcaaggaccg tattgcgcct tcaggcttgg
     5941 ccggacggtt tcacgctgcg agcaagggcc ccacccgcgg ccacgtcgca acctgccaca
     6001 gccggtccac cgcagccttc atttcagcgg gcgaggctgc gccgctgaac gcggccaacc
     6061 gcagcgcctt ggcagtgatc tcctcccggc tgagggtgtt gcctgggtcg cccttgggct
     6121 cgtcgacgcg ccccttcagg acccggccgt cggtggtgtg gaccgtcacc ttgccgatcc
     6181 agcgccgagg gtaggcggcg tcgacttcgg cgtccagctc catggtcacg cggtcacgca
     6241 gcgcctgggt ggactcgtcg aggaagtgtg cttcgaactc gttcagcccc gcctggcgga
     6301 agcgcgccac cagggccagg acggtgccca tcgagaactt gctctggtgc accgtggcgg
     6361 gccgcgtgac gggacccagc acgtcgatgg caccctggtg cacatgcgtg accacccggt
     6421 cgaggtcctc cggcgccagc gcgtgcgcct gcattacctg cagcagcgcg tcggcggccg
     6481 gatgggtgtg gcggcagctg gcatggaact tgaaagatgt ttcgagcgtg gcccagcggt
     6541 cgccgagccc gtcgtcgagc cggctcggat cggcgtccgt cgacatgccg gcggccatgc
     6601 cctgggctcc ctcgagaatt tcggcggccc cggtgaatcc ttcctgcgcc aggcaggcgg
     6661 ccatcaggcc ggccgcggcg gcatgggcgg tatgcaactg cttgctgtcc gctgccgtgc
     6721 gcaggaattc ccacaggccg gccgactgtg tgcccgccga accaaaggca tgctgcatct
     6781 gcgcagcgtc cagccccagc agattgccgg cgcaggccgc ggcggcaagg gtacccgcgg
     6841 tccctgtggt gtggaacacc ttgtagtgag accggccgag aaactcaccc acgcggatgc
     6901 cgacttcgta acctgcgaca gcggccgtca gcagttgttt gccgctggcg ccgcgcgcct
     6961 gcgccagcgc cagcgccacc gggaacacca cggtggccgg gtgaaagacc gagccgttgt
     7021 gcacgtcatc ctgttcggcc acatgcgacg cggcggcatt ggccatggcg gcaaagtagg
     7081 ggctggtgaa ggtgcgccgc accagcagct ccgacccgcc ctgccccgcc ttcgacacag
     7141 ggcccatttc gcaggcaaaa cccgcaatcg tttcgaccgg acgcgcgtcc tttccggcaa
     7201 tggccgagcc gaaccagtcg accaggaggt cttcggcctt acgcaggacc gccgccggaa
     7261 tggcgtcgaa tttcaggccg gcggcaaacg cggcgagttc agtggtgcgt gtgtgcatga
     7321 tgtgtggatg ccgcgacggg tcatcgaaga aaccccgcct gggcgttctt tggttgctat
     7381 atggcgcgac tttgctgcag cgcggcaatc tcggcgtcgc cgtgccccag ctcgcgcagg
     7441 atggcctcag tatgctggcc cacggcggga accgggtcca tccggtaatc gaaactgttg
     7501 aagcgtcccg gcggcagcat cgcggggatt tcgcccaccg gtgacccgat ccggcgccag
     7561 cgttcgcgtg ccgccagttg tggatgggcc cacaggccgg ccatgtcgtt cattcgggca
     7621 ttggcaatct gcgcgtcgtc cagacgcgcc accacctgct gagttgtcag cgccgagaag
     7681 gcctgcacga tgatggcggt aagttcattg cggtgctcgt tgcgcctggc gttgctgtcg
     7741 aagcgcgggt cggccgccag agcgacgtcg cgcagcacca attcacagaa aaccttccac
     7801 tcgcgctcgt tctgcaggcc cagcatgacg gtaccgccgt cgcccgcatc gaaaggacca
     7861 tagggataga tgctcgcgtg cgacgcgccg gtgcgcggcg gcggcggcgc cccctcgaag
     7921 gcgtagtaca tcggatagct catccattcg gcgagcgatt cgagcatgga aacatcgatg
     7981 tggctgccct ccccggtctt cgcgcgcagc atcagggccg agaggatgcc cgtgtaggca
     8041 tacatgccgg cggcaatatc ggccaccgag ttgccggcct tgctcggcga ctcgggcgtg
     8101 cccgtaaccg agaggaatcc ggcctcgctt tgaatcagca ggtcataggc cttcttgtcg
     8161 cggtaagggc cgtcttcgcc atagccggag atgtcgcaga cgatgagccg ggggtgttcc
     8221 cggctgagcg cttcatagcc cagtcccatg cgggcggcag cgcccggcgc caggttttga
     8281 accagcacgt cggcaccggc cagcagacgc ttgagcaccg cgagtgcgcc ggcctgtttc
     8341 aggtcgagcg tcaggctttc cttggagcgg ttggtccaca cgaagtgcga agcaaggccc
     8401 ctgaccctgg catcgtagtc acgagcgaag tcccctccgc cgggacgctc gaccttgatg
     8461 acccgggcac cgagatcggc aagctggcgc gtgcagaacg gcgccgcgat cgcgtgttcg
     8521 agggaaacga cggtgatgcc gtccagcggc cgcacggcag tggtcatggc gccgccttca
     8581 gaacgaacgc ggcaggccca gcaggtgctc cgccacgtaa ctcaggatca ggttggtgga
     8641 gatcggcgcc acctggtaca ggcgcgtctc gcggaacttg cgttccacgt cgtactcgtt
     8701 ggcgaaaccg aagcccccgt ggaactgcag gcacgcgttg gcagcctccc acgaggcctt
     8761 ggccgcgagg tacttggcca tgttggcctc ggcgccgcag ggctgatggg catcgaaaag
     8821 ctggcaggcc ttgaagcgca tgaggttggc cgcttcgacc tcgatatagc tctcggcgat
     8881 cgggaactgc acgccctggt tctggccgat cgggcggccg aagaccacgc gctcgctcac
     8941 gtacttgctg acccggtcga tgaaccagta gccgtcgccg atgcactcgg cggcgatcag
     9001 cgtgcgctcg gcgttgaggc cgtccaggat gtacttgaat cccatgcctt cctggccgat
     9061 caggttctca gcgggaattt ccaggttctc gaagaacagc tcgttggtct cgtggttgac
     9121 catgttggga atgggccgca ccgtcaggcc ggccttctcg gcctttcgca ggtccaccat
     9181 gaagatcgac atgccctcgg tttttttctt cacctccgcc aggggcgtgg tacgcgccag
     9241 caagatcatc cagtcggagt gctggacccg cgagatccag actttttgcc cgttgatcac
     9301 gtaacggtcg cccttcttca cggccatggt cttgatcttg gtggtgtcgg tgccggtcgt
     9361 cggctctgtc acccccatgg cctgcaggcg ccattcaccc gaagcgatct tgggcaggta
     9421 gaggtctttc tgggcctgcg agccatggcg cagcagcgtg cccatgttgt acatctggcc
     9481 atggcaggcg ccggcgttgc cgccgctgcg gttgatctct tccatgatga cggacgcctc
     9541 ggcaagtccc aggccggagc cgccgtaatc cgccgggatc aaggcggcaa gccagccggc
     9601 ttttgtcagc gcatcgacga aagcctcggg gtagccgcgc ttctcgtcga tctggcggtg
     9661 gtattcgccc ggaaattcgg cgcacagcgc gcgaacggcg tcgcggatct cctggtattg
     9721 gtcgggagtg gtcttgagca tgtcggtctc tcggattgtg aaaaggtgta ctgcgcgggc
     9781 ggcgttacgc caggtcggcg gttgcctgca tggtcagcca gccttcgtgg tcctgggccc
     9841 acaggcggac ggtcttgcca tcagccgagg gctgggcatt cacgcgcaat gggtgcggat
     9901 cgaagctggg ccgcacggcc ttgaactcga agcgctgcat gaaggcgccg ggtgcgtggc
     9961 ggcgcaccag atcggccagc aaggtcgcaa tcagcggccc gtgcacgaca aggcccggat
    10021 agccttcgac ttcggtcacg tagcgccggt catagtggat ccggtggcca ttgaacgtca
    10081 gcgccgaata gcggaacagc agcacgtcgt cgggcacgat ctcgcgctgc caggccgcgc
    10141 cttgttcagc ggccacaggc gtcggttggg ggtcgcccgg ctgggctgcg gcacggtaca
    10201 cgatgtcgtg ctcttcggtc agcgcgagcc cctgggcgtt gtgtacctcg tgcttgacca
    10261 gcacgaacag caggtcgccg gagcgcccgg ccttgtgggt gacggactcg atgcgcgaga
    10321 tacgctgaat cgcatcgccc accgtgagcg ggttgtccct ggcccat
//
LOCUS       DASWIY010000189         4253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_101960, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000189 DASWIY010000000
VERSION     DASWIY010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101960"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..894
                     /locus_tag="VGA59_07010"
     CDS             103..894
                     /locus_tag="VGA59_07010"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEX9719467.1"
                     /translation="MKVRVLGCSGAIAKDCRTTSFLIDGDLLVDAGTGVGDLTLEEMQ
                     GIGHVMLTHSHLDHVAALPLMIDAIASQLAKPIIVHALAGTIAALKAHVFNNVIWPDF
                     SRIPTPEAPFLSFHEIRVGQTLHIAGKHIEVLPAVHTVPAVGYAVTSGKGCWIFSGDT
                     ERNPAFWRRVNELNVAALVIETAFSNRERDLARRSLHLSPHALADELDCIDRGKSFPI
                     YITHTKPAETELIMSEIQKFDQTQPSGPNVVHDIRWLRAGQEFEI"
     gene            891..3296
                     /locus_tag="VGA59_07015"
     CDS             891..3296
                     /locus_tag="VGA59_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX9719468.1"
                     /translation="MSAVLRPARDSEQEFFDSQMLPPEKRGVSFEVLFYRQLQQVSTR
                     IHETENIDQIMLEASQDICRLLNADRLTLYAVNEDGTAIVSKVKTGLNTSRDLKLPIS
                     AQSLAGYAAFSRQILNLTDVYDDVALKRIHPTLAFLKEVDKRSGYRTKQILVVPILDG
                     DRLHGVLQVINNKSDQPFGELEVEGATQLCKTLATAIRQRLRKADESQARRATKYDGL
                     VVDGVMTADELQQCIQQAREEGKPVEHTLMASFRIRPAQIGPSLARFFRVPYEPFNAG
                     RIRSEILQGPLKREFVQEQRWLPLEESPEGLVVMCTDPEAVRGSRIVPQVFSRFTKFA
                     YRVTTQTEFEETVAQLYGADDGASIDELLADLGGPLDDEGDESGLESAAADNELVKFV
                     NKVIVDAYNQKASDIHIEPLPGKAKTGIRFRIDGSLQPYIEVPSHFRQALVTRLKIMC
                     DLDISEKRKPQDGKIKFKKFGPLDIELRVATIPSSGGVEDVVMRILAAGEPIPLDKLG
                     LTPHNRERVEKTVGKPYGLFYVCGPTGSGKTTTLHSILKFLNKPDTKIWTAEDPVEIT
                     QKGLRQVQINRKAGIDFALVMRAFLRADPDIIMVGESRDKETVSMGIEASLTGHLVFS
                     TLHTNSAPESIVRLLDMGMDPFNFADALLGILAQRLAKKLCDCKQEYTPDADELKLFV
                     AEYAEELRHSSAWKADYAGEAKKLYEAWVKQYGSDGRLKFHKAAGCDKCNQTGYKGRL
                     GLHELLVADDHIKRLIQERARVAELFSAAVETGMRTLKMDGMEKIMLGLTDLKQVRSV
                     CIK"
     gene            3358..4038
                     /locus_tag="VGA59_07020"
     CDS             3358..4038
                     /locus_tag="VGA59_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719469.1"
                     /translation="MRMVMSLIKDVFSASKAETVTRQDLGVKSVLNVGGGSKATAIPG
                     YFAGWRHDLLDIDPRGAPDIVCDARQLEKLPGGVYDAVYCSHNLEHYYRHDGLKVVRG
                     FLHMLNPTGFAEIRVPDIAQVITALHEQQLELDDVLYQSPAGPIAAHDVIYGLQTEIE
                     RSGQDFYAHKTGFTPKSLSKMLLDGGFHKVFLTTDPYLAVHALAFKAEPTIEQRALLG
                     QTWNLGLE"
BASE COUNT          880 a         1291 c         1327 g          755 t
ORIGIN      
        1 aggcacacca gcggcccggc aaagccggtt ccgcggtgtg ccccgcctcg gccgtgcgtt
       61 ttcatgcgcc gcggaaacgc gaatcgcggt ggagcaattg aaatgaaagt gcgcgtgctg
      121 ggctgttccg gagcgatcgc gaaggactgt cgaaccacgt ccttcctgat cgacggggat
      181 ttgctggtgg acgccggcac aggagtgggc gacctgacgc tggaagagat gcagggcatc
      241 gggcacgtga tgctgacgca ttcgcacctc gaccacgtcg ctgcgctgcc gctgatgatc
      301 gacgccattg cttcgcagct ggccaagccc atcatcgtcc atgcgctggc gggcacgatt
      361 gcggcactca aggcgcacgt cttcaacaac gtcatctggc ccgacttcag ccgcattccg
      421 acgccggagg cgccgttcct cagctttcac gaaatcaggg tgggacagac gctgcacatc
      481 gcaggcaagc acatcgaggt cctgccggcg gtccacacgg ttcccgccgt gggctacgcg
      541 gtcacttcgg gcaaggggtg ctggatcttc tcgggcgaca ccgagcgcaa tcccgctttc
      601 tggcgtcggg tcaacgagtt gaatgtggcc gccctggtga tcgaaacggc cttcagcaat
      661 cgcgagcggg atctggccag gcgcagcctg cacctgtcgc cgcatgcgtt ggccgacgag
      721 ctcgattgca tcgaccgcgg caagtccttc ccgatctaca tcacccacac caagcccgcc
      781 gaaaccgagc tgatcatgag cgagatccag aagttcgacc agacgcagcc gtccggaccg
      841 aacgtggtcc acgacatccg gtggctgcgc gcgggccagg agttcgagat atgagcgctg
      901 tcctcaggcc cgcgcgcgat tccgagcagg agttcttcga ctcgcagatg ctgccgcccg
      961 aaaagcgggg cgtgtcgttc gaggtcctgt tctaccgcca gctgcagcag gtctccaccc
     1021 gcattcacga aaccgagaac atcgaccaga tcatgctcga ggccagccag gacatctgcc
     1081 ggctgttgaa tgcggatcgc ctgacgcttt atgcggtgaa cgaagacggc accgccatcg
     1141 tctccaaggt caagaccggc ctgaacacca gccgcgacct gaagctgccg atcagcgccc
     1201 agagcctggc gggctacgcc gccttcagcc ggcagatcct gaacctcact gacgtctacg
     1261 acgacgtggc gctcaagcgc atccacccga cgctcgcctt tctcaaggaa gtcgacaagc
     1321 ggtcgggcta tcgcaccaaa cagattctgg tcgttcccat tctcgacggg gaccggctgc
     1381 atggcgtgct gcaagtcatc aacaacaaga gcgaccagcc gttcggcgaa ctcgaggtcg
     1441 aaggcgctac ccagttgtgc aagacgctgg ccaccgccat ccgccagcgc ctgcgcaagg
     1501 ccgacgagag ccaggcgcgg cgggcgacca agtacgacgg cctggtcgtc gatggcgtga
     1561 tgaccgccga cgaattgcag cagtgcatcc agcaggcgcg cgaggagggc aagccggtcg
     1621 agcatacctt gatggcgagc ttccggattc gccccgcgca gatcgggcct tcgctggcca
     1681 ggtttttccg cgtgccttat gagcctttca acgccggacg catccgctcg gagatcctgc
     1741 agggtccgct caagcgcgag ttcgtccagg agcagcgctg gctgccgctg gaagagtccc
     1801 cggaggggct ggtggtcatg tgcaccgacc cggaagccgt gcgcggctcg cggatcgtgc
     1861 cgcaggtgtt ttcgcgtttc acgaaattcg cctaccgggt cacgacccag accgagttcg
     1921 aggagaccgt ggcgcagctt tacggcgccg acgacggcgc ctcgatcgac gagctgctgg
     1981 ccgacctggg cgggccgctg gacgatgagg gcgacgagtc ggggctcgaa tcggcggcgg
     2041 ccgacaacga gctggtgaaa ttcgtcaaca aggtcatcgt ggatgcctac aaccagaagg
     2101 cctccgacat ccacatcgag cccctgccgg gcaaagccaa gaccgggatc cggtttcgca
     2161 tcgacggcag cctgcagccc tatatcgagg tcccatcgca ttttcgccag gctctcgtga
     2221 cgcggctgaa gatcatgtgc gacctggaca tctcggagaa gcgcaagccg caggacggca
     2281 agatcaagtt caagaaattc gggccgctcg acatcgagct gcgggtcgcc accattccgt
     2341 catccggcgg cgtggaagac gtggtgatgc gcatcctggc ggcgggcgag ccgatcccgc
     2401 tggacaagct gggcctgacg ccccacaaca gggagcgggt cgaaaagacc gtgggcaagc
     2461 cctatggact gttctatgtg tgcggtccca ccgggtcggg caagaccacc acgctgcact
     2521 cgatcctgaa attcctgaac aagcccgaca ccaagatctg gacggccgag gacccggtcg
     2581 aaatcaccca aaagggcctg cgccaggtcc agatcaacag gaaggcgggc atcgacttcg
     2641 ccctggtgat gcgggcgttc ctgcgggccg accccgacat catcatggtc ggcgaatcgc
     2701 gcgacaagga aaccgtttcg atgggcatcg aggcgtcgct gaccgggcac ctggtgttct
     2761 ccacgctgca cacgaactcg gcccccgaat ccatcgtccg cctgctggac atggggatgg
     2821 accccttcaa ttttgccgac gccctgctcg ggatcctggc gcagcgcctg gccaagaagc
     2881 tgtgcgactg caagcaggag tacacccccg acgccgacga actcaagctg ttcgtcgccg
     2941 aatacgctga agagctgcgg cactcgtcgg cctggaaagc cgattacgca ggagaagcca
     3001 agaagctcta cgaagcctgg gtcaagcagt atggaagcga tggccggctg aagttccaca
     3061 aggcagcggg ctgcgacaag tgcaaccaga ccggctacaa gggccgcttg gggctgcatg
     3121 aactgctggt cgccgacgac cacatcaaac gcctgatcca ggaacgggcg cgggttgccg
     3181 aacttttttc ggcagcggtg gagacgggca tgcgcacgct caagatggac ggcatggaaa
     3241 aaatcatgct gggattgacg gatctgaagc aggtgcggtc ggtttgcatc aaatgacagg
     3301 aatgggtggt tggggtgctc gcaaatctga ccacgccatg aggatcgggc gggacaaatg
     3361 cggatggtga tgtcgctgat aaaggatgtt ttctcggcat ctaaagccga gacagtcaca
     3421 aggcaagacc ttggcgtcaa gagtgtgttg aatgtgggcg gagggagcaa ggcgacggcg
     3481 attccaggct acttcgccgg ctggcggcat gacttgcttg atatcgatcc gcgaggtgca
     3541 cccgacattg tttgcgatgc gcgccaactg gaaaaacttc cagggggcgt ctacgatgcc
     3601 gtctattgct cgcacaatct tgagcattac tatcgtcatg acgggctgaa ggtggtccgc
     3661 gggtttcttc acatgctcaa cccaaccggg tttgctgaaa ttcgtgtccc tgacattgcc
     3721 caggtaatta ccgcattgca tgagcaacag cttgaattgg atgatgtgtt gtatcaaagt
     3781 cctgccggcc cgatcgcggc gcacgacgtc atttacgggc tccagaccga gatagaacga
     3841 agcggccagg acttctacgc tcacaagacc gggttcacgc caaagtcgct aagcaaaatg
     3901 ctgctggacg gcggtttcca caaagtgttt ctcaccacgg atccgtacct cgcagttcat
     3961 gcgcttgcat tcaaggctga accaaccatt gagcagcgcg cccttctcgg gcaaacgtgg
     4021 aatttggggc tggaatgact cgcatcagcc ccgcgctcat atgaagcgcc cgaggtgttt
     4081 caggtccgaa ttcagatgga cggtttggaa aaaacatgct ggtattgacc gatctgaagc
     4141 agggtgcgat ccgtcctgca actagtagct caagtagact taagtgctga caaaaatgtc
     4201 gcggcgtctt tggccttggg tcactttttg ccatctggcg gcgcagcaac agg
//
LOCUS       DASWIY010000190         6126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3418871, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000190 DASWIY010000000
VERSION     DASWIY010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3418871"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VGA59_07025"
     CDS             complement(<1..397)
                     /locus_tag="VGA59_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX9719470.1"
                     /translation="MAAVFDKPSLRRRLLPSLQGFDGPLAFAVFLLACAGLLTMYSSG
                     FDHGTRFADHGRNMLIAGFLMFVVAQVPPQRLMSFALPLYVIGVALLVAVMIFGITKK
                     GARRWINVGIVIQPSEILKIATPLMLAWWF"
     gene            complement(446..2302)
                     /locus_tag="VGA59_07030"
     CDS             complement(446..2302)
                     /locus_tag="VGA59_07030"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9719471.1"
                     /translation="MILQRWRNLAAAACAFLAMAAGAVTTELTQARATITVDGKTTVA
                     QVVLPYHLDREQGTRPAQAVFEVPFRSTSQRDELHGLYLARVGNSAQIWLNDSLLAEL
                     GDIGQSNAADYSKAPQYVSIPAQLLRADNVIRIHVRADGGRRAGLSSLVIGPDQEVIP
                     IFEEASRWRMSASMGVAIFSLLVGTLALGLWAAHVEGAPGRVRGAADPDVYDGIYLSA
                     GLAEFSWALRVGDTAINNPPLPWPAWGVVVTAAFAGWICCMALFCHHVAGWHRHPSMV
                     WMRIFLAALFGTSILASALSYSLYQPLFLTIWLGCANLFFIIYAAVYLVTALRSSTPA
                     RLLVAFAGALNVAVGVRDWIAIRISGDYSQSTWIRYSSVLFGLALGYIVITRFRAASA
                     QANDLMDNLASRVSEKEQELFLSYARLNRLGREHERAAERSRILRDMHDGVGSHLTSA
                     IRQLESGRAEPDQVLQTLRDSLDQLKLSIDAMNLPPGDVTALLANLRYRLEPRFSALD
                     MELQWHVDDLAAVPGIDGAAMRHLQFMLFEALSNVLQHARARVLRIEASTTGSGALLR
                     IIDDGCGFDVGQPLRKGLRSMHDRAAAIGARLSLESRPGRTVVGIALSEKAI"
     gene            complement(2325..3044)
                     /locus_tag="VGA59_07035"
     CDS             complement(2325..3044)
                     /locus_tag="VGA59_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9719472.1"
                     /translation="MWRVLIVEDDANMREFFAASVRRCDGLALAGSLGTVADARAWLD
                     NSAHCLDVLLTDLGLPDGSGLEVIRHAIARNPACEALVISMFGDEENVLASIEAGALG
                     YIHKDSTPQDIADAILQMKNGASPISPMIARRVLAKYRDVMSGASEHPGDRARKNRVE
                     PQATPHSPGGRALSPREQEVLELIARGFSYADIARLKGVSVHTVQTHIKHLYGKLAVR
                     SKNEAVYEATQLGLLQPPARV"
     gene            3198..3791
                     /locus_tag="VGA59_07040"
     CDS             3198..3791
                     /locus_tag="VGA59_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEX9719473.1"
                     /translation="MAVTHLLYLHGFRSSPRSTKAQKVAARVRERHSRVQWWCPQLPA
                     SPCAALDMLLRETGDWPRNAMAVVGSSLGGFYATCLAEATGCRAVLLNPAIDPARDLA
                     GYTGEHAAWHDPDEHFVFEPRFVDELRALECGPVAHPENYFAIIAKGDEVLDWREMTG
                     RYPGAKIKLLEDGDHALSDFDRHIDEVFEFLDLSPRA"
     gene            4074..>6126
                     /locus_tag="VGA59_07045"
     CDS             4074..>6126
                     /locus_tag="VGA59_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX9719474.1"
                     /translation="MFVLFEEAGKFQAGRVLSQAEASAQVELDSGKRVKVKTGNILLK
                     FDRPSPTELMREAQALAHTIELELAWEFAPENEFGFDQLARDYFSDKATTAQQAAMLL
                     RLFEAPHYFRRAGRGRFRKAPAEILQQALAAIEKKKQIQAQITAWAEELAGGVCPQPI
                     REQLFKILFKPDKNAPEYKAVVEASRGTHIAPLELLQKAGAIASPYQFHWQRFLFENF
                     PKGTAFPPLQAPAIRDELPLAGVQAFSIDDSSTTEIDDALSVQGLGSGTVTVGIHIAA
                     PGLALQPSAPLDQVARQRLSTVYMPGHKVTMLPEDVVQAYTLQEGRDCPAVSLYVTFD
                     EATLAQKETQTRLERVPIVANLRHDQLDAVVTQQWLESSAEEGHDAGQALASLREPLS
                     FLHRLASRLKSLREVVRGKPESFNRPDYNFKLMGNNGAEPGGNEQVQIAVRRRGAPLD
                     LIVSEAMILANSTWGGWLAELGVPAIYRSQASMAPGVKVRMGTKALPHAGIGVRSYAW
                     STSPLRRYTDLVNQWQIIAAARHGRTAALAAPFKPKDAELFSVISGFDAAYAAYNAHQ
                     AGMERFWTLKYLLQEGLEELVGTVFKEGFLRTDDLPLVLPVMGGADLPRGARVRVKLG
                     QIDLITLDVKGTVIERLDVAPAPLDSEEEEAEDEPVAGPIAIAVDVNDTEETSAATID
                     NP"
BASE COUNT         1085 a         2040 c         2034 g          967 t
ORIGIN      
        1 ggaaccacca ggccagcatc agcggcgtgg cgatcttcag gatttcgctg ggctggatca
       61 cgatgccgac gttgatccag cgcctggctc ccttcttggt gatgccgaag atcatcacgg
      121 ccaccagcag ggccacgccg atcacgtaca gcggcaacgc aaaactcatg agccgctggg
      181 gcggaacctg ggccaccacg aacatgagaa agccggcgat cagcatgttg cggccgtggt
      241 cggcaaacct cgtgccgtgg tcgaagccgg aggaatacat ggtcagcagg ccggcgcagg
      301 cgagcaggaa gacggcgaat gcgagcggtc cgtcgaagcc ctgcagcgac gggagcaggc
      361 gccgcctgag ggagggttta tcgaagactg cggccatggt cgcgattatc ctgtgccggt
      421 ggccccgccg ccggtaaccc ctggttcaga tagccttttc ggacagtgcg atgccgacca
      481 cggtgcggcc cggccggctt tccagtgaca ggcgagcgcc aatggcggcg gcgcgatcgt
      541 gcatcgagcg caggcctttg cgcagcggct ggccgacatc gaacccgcac ccgtcgtcga
      601 tgatcctgag cagggccccg ctcccggtcg tggacgcttc gatgcgcagc acccgggccc
      661 tcgcatgctg cagcacgttg gacagggctt cgaacagcat gaattgcagg tggcgcatcg
      721 ccgccccgtc gatgccggga acggccgcaa ggtcgtccac atgccactgc aactccatat
      781 ccagggccga gaatctcggc tccagccggt aacgcaggtt cgccagcagc gcagtcacgt
      841 cgccaggcgg caggttcatc gcgtcgatgg acagcttgag ctggtccagc gaatcgcgca
      901 gcgtctgcag cacctggtcg ggctcggcgc ggcccgactc gagctggcgg atcgccgatg
      961 tgaggtgcga tccgacgccg tcatgcatgt cgcgcaggat gcgtgagcgt tcggccgcac
     1021 gctcgtgctc acggcccagc cggttgagcc gtgcgtagct caggaagagt tcctgttcct
     1081 tctccgaaac acgggacgcc aggttgtcca tcaggtcgtt ggcctgggcg ctggcggcac
     1141 ggaagcgcgt aatgacgatg tagccgagcg ccaggccgaa cagcaccgat gaatagcgga
     1201 tccaggtgga ttgggaatag tcgccgctga tgcggatggc gatccagtcg cgcacgccga
     1261 cggcgacgtt caacgcgccg gcgaacgcca cgagcagcct cgccggggtg gacgagcgca
     1321 acgcggtgac gaggtatacc gccgcataaa tgatgaagaa aagattggcg cagccgagcc
     1381 agatcgtgag aaacaggggt tggtacaggg agtaggacag cgccgaggcc aggatcgagg
     1441 tgccgaacag ggccgccagg aagatgcgca tccacaccat ggaagggtgg cggtgccagc
     1501 cggcgacatg gtggcaaaac agcgccatgc agcagatcca gccggcgaac gcggctgtga
     1561 ccaccacgcc ccaggccggc cacggcagcg gaggattgtt gatcgctgtg tcgcccaccc
     1621 gcagcgccca gctgaattcg gccagcccgg cgctcaggta gatgccgtcg tacacatccg
     1681 gatcggctgc ccctcggacc cggcccggcg ccccttcgac gtgcgcagcc cacaggccaa
     1741 gcgccagcgt gcccaccagc aggctgaaga tggccacccc catcgacgcg ctcatgcgcc
     1801 aacgggaggc ctcctcgaag atagggatga cttcctgatc gggtccgatc accagcgaag
     1861 acagcccggc gcgccggccg ccgtcggcgc gcacatggat cctgatcacg ttgtcggccc
     1921 gcagcagttg cgccggaatg gacacgtact gcggcgcctt cgaatagtcg gcggcgttgc
     1981 tttgcccgat gtcgccgagt tccgccagca ggctgtcgtt gagccatatc tgggcgctgt
     2041 tgcctacccg tgccagatag aggccgtgaa gctcgtcacg ctgggacgtg gaccgaaacg
     2101 gcacctcgaa aacggcttgc gcgggacggg tgccctgctc gcggtccagg tgatagggaa
     2161 gcaccacctg ggcgacggtg gtcttcccgt ccaccgtgat cgtggcgcgc gcctgggtca
     2221 gctcggtcgt gacggcgccc gcagccatcg ccagaaaggc acaggcggct gccgccagat
     2281 ttcgccaccg ttgcaagatc atggggacca tggcgcggcc tgcctcacac gcgtgcgggc
     2341 ggttgcagca gacccagctg cgtggcctcg tacactgctt cgttcttgga ccgcaccgcc
     2401 agttttccat agaggtgctt gatgtgcgtt tgcaccgtgt gcacgctcac acccttcagc
     2461 cgggcgatat cggcgtagga gaagccccgc gcgatcagtt ccagcacctc ctgctcgcgc
     2521 ggcgacagtg cccgtccgcc cggggagtgc ggcgtcgcct ggggctcgac ccggttcttg
     2581 cgcgcccggt caccgggatg ttcggacgcc ccactcatga catcgcggta cttggccagc
     2641 acccggcgcg cgatcattgg cgagatcggc gacgcgccgt tcttcatctg gaggatggcg
     2701 tcggcgatgt cctgcggagt cgaatccttg tggatgtatc cgagtgcgcc ggcctcgatg
     2761 ctggccagca cgttctcctc gtcgccgaac atcgagatga ccagggcctc gcaggcgggg
     2821 ttgcgggcta tcgcatggcg gatgacctcc aggccgctgc cgtccggcag gcccaggtcg
     2881 gtcagcaaca cgtccaggca atgagcggaa ttgtccagcc aggcgcgagc gtccgcgacc
     2941 gtgccgaggc tgcccgccag cgcgaggccg tcgcaacgac gcacgctcgc ggcgaagaac
     3001 tcgcgcatat tcgcgtcgtc ttccacgatc aatacacgcc acatccgttt tcccctctgt
     3061 ccggcagcgg gcgcggccgg ccctgctcaa cagtatgcat ccagcgatag acggtgccta
     3121 gtgtcctgtc acacaaatat ctgtactctc gttcgagtcc atgcggtgat gatcggcggc
     3181 ttatgggaac atccggcatg gccgtcaccc acctgctcta tctccacgga ttccgctcct
     3241 cgccccgctc gaccaaggcg caaaaagtcg ccgcccgcgt gcgcgaacgc cattcgcggg
     3301 ttcaatggtg gtgcccgcag ctgccggcct cgccatgcgc ggcgctggac atgctgctgc
     3361 gggaaaccgg cgattggccc aggaacgcga tggccgtcgt cggctcctcc ctgggcggct
     3421 tctacgcgac ctgcctggcc gaagcaaccg gctgccgtgc cgtcctgctc aacccggcga
     3481 tcgacccggc gcgcgacctg gccggctaca ccggggagca cgccgcctgg cacgatcccg
     3541 acgagcactt cgtgttcgag ccgcgctttg tcgatgaact gcgcgccctc gaatgcggcc
     3601 ccgtcgccca tccggaaaac tacttcgcca tcatcgccaa gggcgacgaa gtcctcgact
     3661 ggcgcgagat gaccggccgt tatcccggcg cgaagatcaa gctgctggag gacggggacc
     3721 acgcgctctc cgacttcgac cggcacatcg acgaggtgtt cgaattcctt gacttgtcac
     3781 cccgggcgtg acctgggctt gacccggggt ccaggacttc ctggattgcg gggccgggcc
     3841 ggagcgcgat gcctcggtac gtgcatagcg taggcccggc attgggctcg ctgcactgga
     3901 cagctgcgcg ccccgggcgt tccgcgagcc cagcccgcaa tgacagggga aggttgtcat
     3961 cccggactcg atccgggatc catgtcttct gaattcatgg attgcgggtc aggcccgcaa
     4021 tgacagcgcg ctttacgcga gggatgactg gctcgcatgg gacaatcgat cccatgttcg
     4081 tattgtttga agaagccggg aagttccagg cgggccgggt gctgtcgcaa gccgaggcat
     4141 cggcgcaggt cgagctcgac agcggcaagc gcgtgaaggt gaaaaccggc aacatcctgc
     4201 tgaaattcga ccggccctcc cccaccgagc tgatgcgcga ggcgcaggcc ctggcacaca
     4261 ccatcgagct ggagctggcc tgggaattcg cgcccgaaaa cgaattcggt ttcgaccagc
     4321 tggcgcgcga ctatttcagc gacaaagcca cgacggcgca acaagccgcg atgctgctgc
     4381 gcctgttcga agcgccgcac tactttcgcc gcgccggcag ggggcgcttt cgcaaggcgc
     4441 cggccgagat cctgcagcag gcgctggcgg cgatcgaaaa gaagaaacag atccaggccc
     4501 agatcacggc ttgggccgag gagctggccg gcggcgtctg cccgcaaccg atccgcgagc
     4561 agctgttcaa gatcctgttc aagcccgaca agaatgcgcc cgagtacaag gccgtggttg
     4621 aggcttcccg cggcacccac atcgcgcccc tggagctgct gcaaaaagcc ggcgccatcg
     4681 cgtcgcccta ccagttccac tggcagcgat tcctgttcga aaacttcccg aaaggcacgg
     4741 ccttcccgcc cttgcaggcg ccggcgatcc gggacgaatt gccgctggcc ggcgtgcagg
     4801 cgttctccat cgatgactcc agcaccaccg aaatcgacga cgcgctgtcg gtgcagggcc
     4861 tgggctccgg caccgtcacg gtcggcatcc atatcgcggc gccggggctg gcgctgcagc
     4921 cgtcggcccc gctcgaccag gtagcgcgcc agcggctgtc cacggtctac atgccgggtc
     4981 acaaggtcac catgctgccc gaggatgtgg tgcaggcgta cacgctgcag gagggccgcg
     5041 actgcccggc cgtttcgctc tacgtgacct tcgacgaggc cacgctggcc caaaaggaaa
     5101 cccagacccg gctcgaacgc gtccccatcg tcgccaacct gcgccacgac cagctcgatg
     5161 cggtcgtcac gcagcagtgg ctggaaagct cggccgaaga agggcacgac gcggggcaag
     5221 cgctggcgag tcttcgcgag cccctgtcct tcctgcatcg gctggcgagc cggctgaagt
     5281 cgctgcggga ggtggtgcgt ggcaaaccgg aatcgttcaa ccggcccgat tacaacttca
     5341 agctgatggg caacaacgga gccgagcccg gcggcaacga gcaggtgcag atcgccgtgc
     5401 gccggcgcgg cgcgccgctg gacctgatcg tgtccgaagc gatgatcctg gccaacagca
     5461 cctggggcgg ctggctggcc gagctcgggg tgccggcgat ctaccgcagc caggccagca
     5521 tggcgccggg ggtcaaggtg cgcatgggca ccaaggcgct gccgcacgcc ggcatcggcg
     5581 tcaggagcta cgcctggagc acctcgccgc tgcggcgcta caccgacctg gtgaaccagt
     5641 ggcagatcat cgcggccgcg cgccatggcc gcacggccgc actggccgcg cctttcaagc
     5701 ccaaggacgc cgagctgttc tccgtcatct cgggtttcga cgcggcgtat gccgcgtaca
     5761 acgcccacca ggccggcatg gaacgcttct ggacgctcaa gtacctgctg caggagggcc
     5821 tggaggaact cgtgggtacc gtgttcaagg aaggcttcct gcgtacggac gaccttcctc
     5881 tggtcctgcc cgtgatgggc ggcgccgacc tgccgcgcgg tgcccgggtg cgggtgaagc
     5941 tcggtcagat cgacctgatc acgctggatg tcaagggcac ggtcatcgaa cgcctggatg
     6001 tggcgccagc gccactggat agcgaggagg aggaggcgga ggacgagccg gtggccggac
     6061 cgattgccat cgccgtcgat gtcaacgaca ctgaggaaac ttccgccgcc accatcgata
     6121 acccgt
//
LOCUS       DASWIY010000191         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_481548, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000191 DASWIY010000000
VERSION     DASWIY010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481548"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VGA59_07050"
     CDS             <1..280
                     /locus_tag="VGA59_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX9719475.1"
                     /translation="FYAVHRERPFFNDLVAFMTSGPVMIQVLEGDDAIARNRELMGAT
                     NPQQAAAGTIRADFAQSIEANAVHGSDAPETARVEIACFFGDDEIYSR"
     gene            344..1492
                     /gene="rlmN"
                     /locus_tag="VGA59_07055"
     CDS             344..1492
                     /gene="rlmN"
                     /locus_tag="VGA59_07055"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX9719476.1"
                     /translation="MTASASPSNFVASLRGNLPLNLLDFDEQGLRAFFASIGEKPFRA
                     QQLLKWIYHQGITDFAAMTNLGLDLRQQLQQVALIQPPRVLNEQVSTDGTTKWLMGFG
                     GGNAVETVFIPEPSRGTLCISSQVGCALNCTFCATGAQGFSRNLSTAEIIGQVWQAAR
                     ALGHERNGQRRITNVVLMGMGEPLLNFEAVVPALSLLRDDLGFGLAAKRVTLSTAGLV
                     PGIDRLRDTIDVALAVSLHAPEDRLRETLVPLNRKYPIRELMRACADYVSDKHKRSVT
                     FEYTLIDGVNDHPEHARQLVKLLRRLPSKLNLIPFNPFPGTRYRCSPEERIREFQSIV
                     MEGGLIATVRKTRGEDIDAACGQLVGRVLDRTRRSRILREQSQGGLAQ"
     gene            1489..>2105
                     /gene="pilW"
                     /locus_tag="VGA59_07060"
     CDS             1489..>2105
                     /gene="pilW"
                     /locus_tag="VGA59_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEX9719477.1"
                     /translation="MKAMSGAPTPILALTLILLVLLAGCSSTPNQSDKEMRKAAETNT
                     SLGRQYMERGQHEIALEKLKRAVAYDKTYAPAHSMLAILYETIGKPEQAGEEYRKAVQ
                     YDPEGGEVNNNYGAYLCGIGRQKEAEPYFRVAIEDPFYQTPEVALSNAGACALAQGDL
                     DKAETFLRQSLEHDAKLGAALLPMAEVSYRKESFLQARAFLQRYEA"
BASE COUNT          400 a          681 c          673 g          351 t
ORIGIN      
        1 gttctacgcg gtgcaccggg aacggccgtt cttcaatgac ctggtagcct tcatgacctc
       61 cggccccgtg atgatccagg tgctggaggg cgatgacgcc atcgcccgca accgcgaact
      121 gatgggcgcg accaatccgc agcaggccgc ggccggcacc attcgcgcgg acttcgccca
      181 aagcatcgag gccaacgcgg tgcacggctc ggatgcgccg gaaaccgcca gggtggaaat
      241 cgcctgcttt ttcggcgacg acgagatcta ttcccgctga agcattcgcc gcagccgccg
      301 aacatgctca cgcccatgcc cacgcccccg acataacctc gccatgaccg catccgccag
      361 tccatccaac ttcgtggcca gcctccgggg caacctgccg ctcaatttgc tcgatttcga
      421 cgagcaaggc ttgcgcgcgt tctttgcgtc catcggcgaa aaaccgttcc gggcgcagca
      481 gctgctgaaa tggatctacc accagggcat cacggacttt gccgcgatga ccaacctcgg
      541 cctggacctg cggcagcaac tccagcaggt tgcgctgatc caaccgccgc gcgtgctcaa
      601 cgagcaggtc tcgacggacg gcacgaccaa gtggctgatg ggcttcggcg gcggcaacgc
      661 ggtggaaacg gtattcatcc cggaacccag ccgcggcacg ttgtgcattt cctcgcaggt
      721 gggctgcgcg ctgaactgta ccttttgcgc caccggagcg cagggcttca gccgcaacct
      781 gagcaccgcg gagatcatcg gccaggtctg gcaggccgcg cgggccctcg gccacgaacg
      841 caacggccag cgccgcatta ccaacgtggt gctgatgggc atgggcgagc cgttgctgaa
      901 cttcgaagcg gtcgtgccgg ccctgtcgct gttgcgcgat gatctgggct tcgggctggc
      961 cgccaagcgg gtgaccctgt cgaccgcggg gctggtgccc ggcatcgacc gcctgcggga
     1021 caccatcgac gtggcactgg cggtgtcgct gcacgcgccg gaagaccgct tgcgcgagac
     1081 gctggtgccg ctgaaccgca agtacccgat acgcgaactg atgcgcgcct gtgccgacta
     1141 cgtttccgac aagcacaagc gctcggtcac ctttgaatac accttgatcg acggcgtgaa
     1201 cgaccacccg gaacatgcgc ggcagctcgt caagctgttg cggcgcctgc ccagcaagtt
     1261 gaacctgata ccgttcaacc cgttcccggg aacgcgctac cgctgctccc ccgaggagcg
     1321 cattcgcgag ttccagtcca tcgtgatgga aggcggcttg atcgccacgg tgcgcaagac
     1381 acgcggtgag gacatcgacg cggcttgtgg ccagttggtg ggccgggtgc tggaccgcac
     1441 ccggcgcagc cggatacttc gcgagcagtc acagggaggc ctggcgcaat gaaagcgatg
     1501 agcggagccc ctactccgat tttggctctg accctgatcc tgctggtcct gctggcgggc
     1561 tgctcctcga cacccaacca gtcggacaag gaaatgcgca aggcggcgga gacgaatacg
     1621 tcgctggggc ggcagtatat ggagcgaggg cagcacgaga tcgcgctgga gaagctcaag
     1681 cgggcggtcg cttacgacaa gacctacgct ccggcgcaca gcatgctcgc catcctttac
     1741 gaaaccatcg gcaagccgga gcaagccggc gaggagtacc gtaaggctgt gcaatacgat
     1801 cccgagggtg gtgaggtcaa caacaactac ggcgcctatc tctgtggcat cggcaggcaa
     1861 aaggaggccg agccgtattt ccgggttgcg atcgaggatc cgttctacca gacgcccgaa
     1921 gtcgcgctgt cgaacgcggg ggcctgtgcc ctggcgcagg gcgatctgga caaggccgaa
     1981 acctttttgc gacaatcgct tgaacatgac gctaaactcg gagctgcgct gttgccgatg
     2041 gcggaggtga gttaccgcaa ggagtcgttc ttgcaggcgc gggcgttcct gcaacgctac
     2101 gaagc
//
LOCUS       DASWIY010000192         5959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_487504, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000192 DASWIY010000000
VERSION     DASWIY010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487504"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1155)
                     /gene="glnL"
                     /locus_tag="VGA59_07065"
     CDS             complement(85..1155)
                     /gene="glnL"
                     /locus_tag="VGA59_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEX9719478.1"
                     /translation="MTQPAPISVRFQSLDLLATLVAVVGSDGAVLFANAALEDALGSS
                     RRTILGSNFPQFFTEPHVLRNALEAAGSNEFAALRYDAWLVRPHSEPLPVHVVVAQTE
                     KQGEIVVELLPLEAQARQDREERLIDQAKANKELIRNLAHEIKNPLGGIRGAAQLLEM
                     EIESRELKEYTRVIIHEADRLQTLVDRLLAPHKRPHVVGDVNIHEVCERVRSLIMAEF
                     PRGLAVVREYDTSIPEFRGDREQLIQAVLNIAHNACQSLSERIAAGDARLVLRTRIAR
                     QVTFGKQRYRLALELHVIDNGPGVPDSIRDRIFFPLVSGREGGSGLGLTLAQTFVGQH
                     HGLIECDSAPGRTDFKILIPLP"
     gene            complement(1160..1654)
                     /locus_tag="VGA59_07070"
     CDS             complement(1160..1654)
                     /locus_tag="VGA59_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719479.1"
                     /translation="MKYAFLIPLAIAVLAANSNAQSRIYRCGNTYTNNAAEAQAKGCK
                     PMEGGHITVVEGTRVHSPTVRVATAPQTVSAGPGQRVDAESQRARDADARVILEAELK
                     KAEARQAELLKEFNNGEPEKLGPEHRNYQKYLDRLAELKTGIARNENDIAGIKRELGR
                     VASK"
     gene            complement(1842..3260)
                     /gene="glnA"
                     /locus_tag="VGA59_07075"
     CDS             complement(1842..3260)
                     /gene="glnA"
                     /locus_tag="VGA59_07075"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX9719480.1"
                     /translation="MAAKSVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPVSHFDE
                     DKFNTGHAFDGSSIAGWKGIEASDMLLMPDPATANIDPFYEETTIFLSCDVLEPGDGK
                     AYDRDPRSIAKRAEAYLKASGLGDTAFFGPEPEFFIFDDVRWGQDMSGSFVEIDEYEA
                     PWNTGKKIDGGNKGHRPTTKGGYFPVPPVDSMQDMRAEMSLILETLGIPVEVFHHEVG
                     GAGQNEIGTKFSTLVQRADWTIWMKYVIMNVANAYGKTATFMPKPLVGDNGSGMHVHQ
                     SVWKDGKNLFAGDGYAGLSDFALYYIGGVIKHARALNAITNPGTNSYKRLVPGFEAPV
                     KLAYSARNRSAAVRIPYVANPKGRRLEIRFPDPSCNPYLAFAAMLMAGLDGVENKIHP
                     GEAATKDLYHLPPEEDAKIPTVCHSLDQALEYLDKGRGFLTKGGVFTDTMIDAYIELK
                     MQEVTRYRMTTHPIEFDMYYSL"
     gene            complement(3477..4301)
                     /locus_tag="VGA59_07080"
     CDS             complement(3477..4301)
                     /locus_tag="VGA59_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX9719481.1"
                     /translation="MTHLPPRPEASEFGLIIVGDEILSGRRADKHMPKVIELLAARGL
                     QLAWAEYVGDAPERITAALRRAFASGDAVFSCGGIGATPDDHTRQCAGEALGVVLELH
                     PQAEALIRERMRDIAREQGTPYEPDRPDNVHRLNMGVFPKGARLIPNPYNKIAGFSVG
                     HVHFVPGFPVMAWPMIEWILDDQYRTLFRARPYAEMSVIVFGSMEATLTPLMEQIERD
                     HPEVKVFSLPSVDHPEYGRHIDLGVKGDPERIAPAYAQLLAGLSGIGVRLGPESVR"
     gene            complement(4276..5166)
                     /locus_tag="VGA59_07085"
     CDS             complement(4276..5166)
                     /locus_tag="VGA59_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEX9719482.1"
                     /translation="MSLFLDSFWRAVAYCLHPRVIALSFLPLVIMVALSLGLGYFYWD
                     AAVAWVRVALESSDLLKSVWSWLQDAGAGNLKTVVAPLIVIFAVTPLIVLVSLLVVAA
                     FMAPALATLVAERRFSQLERKHGGSLAASIAWSLGSTALALVALVVTLPLWLVPPLIL
                     LLPPLIWGWLTYRVMAFDVLADHASREERREVFRRHRMRLFGIGVVTGYMGAAPSLLW
                     ASGALFAAAFVILVPFAIWIYTLVFAFSSLWFAHYCLAALETLRAQPSAAAVPPQFPA
                     PAAAPAPPLALPDDAPSPPA"
     gene            5235..>5959
                     /locus_tag="VGA59_07090"
     CDS             5235..>5959
                     /locus_tag="VGA59_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9719483.1"
                     /translation="MELITVNGVKLEVERLPGGAGMRRAPILFLHEGLGSVSMWRDWP
                     ADVCAAAGRDGFVYSRRGYGHSDPISDVRGAGRLAPDYMHREALEVLPGLLAALGLDA
                     PVLLGHSDGGTIALIYAASFPVAACVVMAPHVIVEDLSVHSIAQARDAYGSGDLRERL
                     ARYHADVDGAFWQWNDIWLSPAFRGFDIRAECRRIEAPVLAIQGEDDPYGTLRQIESI
                     QPTSGPFAAQILPQCGHSPHRDQP"
BASE COUNT         1046 a         1915 c         1905 g         1093 t
ORIGIN      
        1 ttttccagca cgaagcggat ggattggtca tcgtcaacga tccagatcgg cttcatgggc
       61 acctcaagtc acggctcgtt caagttatgg caacggaatc agtatcttga aatccgtcct
      121 ccccggggcg ctatcgcact cgatcagccc atggtgctga ccgacgaagg tttgcgccag
      181 cgtcagtccc agccccgagc cgccctccct gcccgatacc agcgggaaaa agatgcggtc
      241 cctgatcgag tccggcacgc cgggcccgtt gtcgatgaca tgcaattcca atgccagtcg
      301 gtagcgctgt ttgccgaaag tcacctgccg ggcgatgcgc gtgcgcaaga ccagccgcgc
      361 gtcgcctgcc gcgatccttt cggaaagcga ctggcaggcg ttatgagcga tgttgagaac
      421 ggcctggatg agctgctcac ggtcgccgcg aaactcggga atggaggtgt cgtactcgcg
      481 cacgaccgcc aggccccggg gaaactccgc catgatgagc gagcgcaccc gctcgcagac
      541 ctcgtggatg ttgacgtcgc cgacgacgtg cggccgcttg tgcggggcca gcagccggtc
      601 caccagcgtc tgcagccggt cggcctcgtg aatgatgacc cgggtgtatt ccttcagctc
      661 ccgggactcg atctccatct ccagcagctg cgccgcgccg cggatgccgc ccagcggatt
      721 cttgatctcg tgggccaggt tgcggatcaa ttccttgttg gccttggcct ggtcgatcag
      781 gcgctcctcg cgatcctgcc tggcctgcgc ttccaggggt agcagttcca ccacgatctc
      841 gccctgtttt tcggtctggg ccaccaccac gtgcaccggc agcggttcgc tgtgcggccg
      901 caccagccag gcgtcatagc gcagcgcggc gaattcgttg ctgcccgccg cttccagcgc
      961 gttgcgcaag acgtgcggtt cagtgaagaa ttgcggaaag ttcgagccca ggatggtgcg
     1021 gcgtgagctg cccagagcgt cctcgagcgc ggcgttggcg aacaggaccg cgccgtcact
     1081 gccgacgacg gccaccaggg tggccagcag gtccagcgat tggaatcgga ctgaaatggg
     1141 cgccggttgg gtcaagccct cattttgacg caacccggcc gagctctcgc ttgatacccg
     1201 cgatgtcgtt ctcgttgcgc gcgataccgg ttttcagttc ggccaagcgg tccaggtatt
     1261 tctgatagtt gcggtgctcg ggacccagtt tttcgggctc cccgttgttg aattccttga
     1321 gcaactcggc ttggcgcgcc tcggccttct tgagttccgc ctccaggatc acgcgggcat
     1381 cggcgtcgcg cgcgcgctgg ctttccgcgt ccactcgctg gcctgggccg gccgaaacgg
     1441 tctgcggggc cgtggcgact cgcacggttg gggagtgaac gcgcgttcct tccaccaccg
     1501 tgatgtggcc gccctccatg ggcttgcagc ccttggcctg ggcctcggcc gcgttgttgg
     1561 tgtaggtgtt gccgcagcgg tagatacggg actgcgcgtt gctattggct gccagcacgg
     1621 caatggccaa ggggatcagg aaagcgtact tcatcgatgg ctcctcgcac gaaagtgcac
     1681 aggacggctc ttcagtatcg cgcaaaccat caaaaaagcc aaataacccc ttgattccaa
     1741 aaggaaaggg acggctttcg ccgtcccttt cctgccacag actgtggatt tttacagacc
     1801 aaggacgcgt gtccccctca gggggaccgc gttatcgtct gttacaacga atagtacatg
     1861 tcgaactcga tcgggtgcgt cgtcatgcgg tagcgagtca cttcctgcat cttcagctcg
     1921 atatatgcgt cgatcatggt atcggtgaac acgccgccct tggtcaggaa gccgcggccc
     1981 ttgtccaggt attccagcgc ctggtccagg ctgtggcaca cggtcgggat cttcgcgtct
     2041 tcctccggcg gcagatggta caggtccttg gtcgcggctt cgccggggtg gatcttgttc
     2101 tcgacgccat cgaggccggc catcagcatg gccgcgaaag cgaggtacgg gttgcagctc
     2161 gggtccggga agcggatttc caggcggcgg cccttcgggt tggccacgta aggaatgcgc
     2221 accgcggccg agcggttgcg ggctgaatac gccagcttca ccggagcctc gaagccgggc
     2281 acgaggcgct tgtagctgtt ggtgccgggg ttcgtgatcg cgttcaaggc gcgggcgtgc
     2341 ttgatgacgc cgccgatgta gtacagcgcg aagtccgaca agccggcata gccgtcgccg
     2401 gcgaacaggt tcttgccgtc tttccagacc gactggtgca cgtgcatgcc ggagccgttg
     2461 tcacccacga gtggcttggg catgaaggtc gccgttttgc cgtaggcgtt ggccacgttc
     2521 atgatcacgt acttcatcca tatcgtccag tcggcgcgct ggaccagcgt ggagaatttc
     2581 gtgccgatct cgttctgccc ggcgccgcct acttcgtgat ggaacacttc caccgggatg
     2641 cccagcgttt cgaggatcag cgacatctcg gcgcgcatgt cctgcatgct gtcgactgga
     2701 ggcaccggga aatagccgcc cttggtggtg ggacggtggc ccttgttgcc gccgtcgatc
     2761 ttcttgcccg tgttccacgg cgcctcgtac tcgtcgatct ccacgaacga gccggacatg
     2821 tcctggcccc agcgtacgtc gtcgaaaatg aagaactcgg gctccggccc gaagaaggcg
     2881 gtgtcgccca ggcccgaggc cttcaggtag gcctcggcgc gcttggcgat ggaacgcggg
     2941 tcgcgatcat aagccttgcc atcgcccggc tcgagcacgt cgcacgacag gaagatcgtg
     3001 gtctcctcat agaacggatc gatgttggcc gtggccggat cgggcatcag caacatgtcg
     3061 gaagcttcga tgcccttcca gcccgcgatc gacgaaccgt cgaatgcgtg gcccgtgttg
     3121 aatttgtcct cgtcgaaatg cgaaacgggc acagtgacgt gttgttcttt tccgcgggtg
     3181 tcggtgaaac ggaagtcgac aaacttgact tcgttctcct tcaccatctt catcacgtct
     3241 gcgacgctct tggctgccat caggtttctc ctggtttgat ggttgttgaa aatatcgaac
     3301 tgaaatgcga atggggatgc agtttttgtg ccaaagctgg tgcatggcat cggccacgaa
     3361 gctcggcgat tgtccccgat ctttagcgct tttccctaca ggagccttgc tcttttggat
     3421 cagtaacgca ctagtatggt gcatccacag aaatgcacca tactagtgca atctgcttat
     3481 cgcaccgatt cggggcccaa ccggactccg atgccgctca gccctgccag aagctgcgcg
     3541 taggcgggcg ctatccgctc cggatccccc ttgacgccca ggtcgatgtg gcgcccgtac
     3601 tcggggtgat cgacgctggg aaggctgaac accttcactt cgggatggtc gcgctcgatc
     3661 tgctccatca gcggcgtcag ggttgcttcc atcgagccaa acacgatcac ggacatctcc
     3721 gcatagggcc gtgcccggaa aagggtccga tactgatcgt ccaggatcca ctcgatcatc
     3781 ggccacgcca tcaccgggaa accgggcacg aagtggacat gcccgacgct gaatcccgcg
     3841 atcttgttgt acgggttcgg gatcaggcgc gccccctttg gaaacacgcc catgttcagc
     3901 cggtgcacgt tgtccggccg gtccggttca tacggcgttc cctgctcgcg cgcgatgtcc
     3961 cgcatgcgct cgcgaatcag ggcctcggct tgcgggtgca gttccaggac cacgcccagg
     4021 gcctcgcccg cgcactgccg ggtgtggtcg tccggcgtcg cgccgatccc gccgcaggaa
     4081 aacacggcgt cacccgaggc gaaggcacgc cgcaaagcgg cggtgatccg ttcgggcgcg
     4141 tcgcccacgt actcggccca ggccagttgc aggccgcgcg cggccagcaa ctcgatcacc
     4201 ttgggcatgt gcttgtccgc gcgccggccg gagaggattt cgtcgccgac gatgatcagg
     4261 ccgaattcgc ttgcttcagg ccggggggga aggtgcgtca tccggcaatg ctaacggcgg
     4321 cgcgggggct gcggccgggg cagggaattg aggcggcacg gcggccgcgg aaggctgggc
     4381 gcgcagcgtt tccaatgcgg ccaggcaata gtgggcgaac cacagcgaag agaaggcgaa
     4441 taccagcgtg tagatccaga tcgcgaacgg caccaggatg acgaatgcag ccgcgaacaa
     4501 cgcgcccgaa gcccagagca ggctgggcgc ggcgcccatg tagcccgtca ccaccccgat
     4561 gccgaacaac cgcatccggt ggcggcgaaa tacctcgcgg cgttcttccc tgctggcatg
     4621 atcggcgagc acgtcgaaag ccatcacccg ataagtgagc cagccccaga tcagcggggg
     4681 cagcaacagg atcagcggcg gcaccagcca gagcggcagc gtcaccacga gcgccaccaa
     4741 ggcaagggcc gtggaaccca gcgaccaggc gatgctcgct gccagcgagc cgccgtgctt
     4801 gcgctccagc tgcgaaaacc gccgctcggc caccagtgtg gccagcgccg gggccatgaa
     4861 agcggccacg acgagcagcg acaccagcac gatcagcggc gtaaccgcga agatcacgat
     4921 cagcggcgcc acgacggtct tgagattgcc ggcgccggcg tcctgcaacc aggaccacac
     4981 gctcttgagc aggtcggacg actcgagcgc cacccgcacc cacgccacag ccgcatccca
     5041 gtagaaatag cccaggccca gcgacagcgc gaccatgatc accagcggca ggaaggacag
     5101 cgcgatcacg cgcggatgca ggcaataagc caccgcgcgc cagaaagaat cgaggaacag
     5161 gctcatgcac gcagcatagc gcggcgcggg agccgcccgc ccggcgcgca ggcccatccc
     5221 gtaacattgc cctgatggaa ttgatcactg tcaacggagt gaaactcgaa gtcgagcgct
     5281 tgcccggtgg cgccggcatg cggcgcgcgc caatcctctt cctgcatgag gggctggggt
     5341 cggtgtcgat gtggcgcgac tggcccgccg atgtctgtgc cgcggccggc cgcgacgggt
     5401 tcgtctattc gaggcgcggc tatggccatt ccgaccccat ttccgacgtg cgcggcgccg
     5461 ggcgcctagc gccggattac atgcatcgcg aagcgctcga ggtgctgccc ggactgctgg
     5521 ccgcgctcgg gctcgacgcg ccggtgctgc tgggccattc cgacggcggc accatcgcgc
     5581 tgatctacgc cgccagcttt cccgtggcgg cctgcgtcgt catggccccg catgtcattg
     5641 tcgaggacct gtccgtgcat tccatcgcgc aggcgcgcga cgcgtacgga tcaggcgacc
     5701 tgcgggaacg gctggcgcgc taccacgcgg acgtggacgg cgcgttctgg cagtggaacg
     5761 acatctggct gagccctgct ttccgtggct tcgacatccg ggccgaatgc cggcgcatcg
     5821 aagcgcctgt attggcgatc cagggcgagg acgaccccta tggaacgctg cgccagatcg
     5881 agagcatcca gccgacatcg ggtccgttcg ccgcgcaaat attgccgcaa tgcggacatt
     5941 cgccgcaccg cgaccagcc
//
LOCUS       DASWIY010000193         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_488132, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000193 DASWIY010000000
VERSION     DASWIY010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488132"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1355
                     /locus_tag="VGA59_07095"
     CDS             159..1355
                     /locus_tag="VGA59_07095"
                     /inference="COORDINATES: protein motif:HMM:NF025596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Druantia anti-phage system protein DruA"
                     /protein_id="HEX9719484.1"
                     /translation="MPFTAQAVYLNLREVQIEPVAGPDEARFQGLMQAHHYLGALPKI
                     GNTLWYVAHVGGQWLALLSFSAAALKCAARDAWIGWDFRCQYDRLHLVANNSRFLILP
                     EHHVPNLASRVLALCERRLRRDWPQRFGHPLLLLETFVDPTRFAGTLYRAANWREVGA
                     SRGFSRTRAGYSATPATPKRVFVRPLVAQAQKHLSQAVLDPVYRHGESKLMLSAAHME
                     SLPTFFARIPDPRRRHGRRHPLPAVLAIASAATLCGMRGYKAIAEWAQSLGQSARARF
                     RCRYRDGRYEIPSESIIRDVLIRVGPEHLDRALQDWNAAYGVQDQALAIDGKTLRNAI
                     DPEGRQVHVMSVIGHQSGTAHAQKKSALCPPLTNRPNKPTRSEPSSRSSSPSTSPVKP
                     SLPTRC"
     gene            1313..1987
                     /locus_tag="VGA59_07100"
     CDS             1313..1987
                     /locus_tag="VGA59_07100"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEX9719485.1"
                     /translation="MDLAGKTLTADALLTQRALATYLVEKRQAHYLFTVKGNQPRLLA
                     DLQLAFAQRGKPEFQEPLDLAHGRIETRAIWTSTQFNDYLDFPHVGQAYLIERSVVHK
                     KSGKSTTELVYGVTSHSAASANPMQVLALNRNHWAIENGCHWVLDWNWDEDRSCIRTG
                     NGPENITRLRRFAISVIKIKSRQSVAATLRKLQRNVRLAFDYLCMTRNSTRRAAAIGD
                     MVRRTN"
BASE COUNT          406 a          784 c          678 g          366 t
ORIGIN      
        1 ggccattgcc aacaactcgg gcgtgccggt gacgtaccag taggtatcgg tgaccttggc
       61 gtggccaggg cgaattcgtg ctcgctgaaa atattggcga atccagaagg ttgttgctga
      121 ttctggacat ttgaacgctg acggagtaga aatgggcggt gccattcacc gcgcaggccg
      181 tctacttgaa ccttcgagaa gtgcagatcg agcccgttgc ggggccggat gaagcccgct
      241 tccagggctt gatgcaggcc catcactacc tcggcgcctt gccgaagatc ggcaacacgc
      301 tgtggtatgt cgcgcatgtc ggcggccaat ggctggccct gctcagcttt tcggcggcgg
      361 cgctcaagtg cgcggcgcgc gatgcgtgga tcggctggga cttccggtgc cagtacgacc
      421 gcctgcatct ggtcgccaac aacagccgct ttctgatcct gcccgagcac cacgtcccga
      481 atctggcctc gcgtgtgctg gcgttgtgcg aacgccgctt gcgccgcgac tggccgcagc
      541 gcttcggcca tccgctgctg ctgctggaga ccttcgtcga cccgacgcgc ttcgccggca
      601 cgctctaccg cgccgccaac tggcgcgagg tgggcgccag tcgcggcttt tcgcgcacgc
      661 gggccggcta cagcgcgacg ccggcgacgc ccaagcgggt gttcgtccgt cctctggtcg
      721 cccaggcgca gaaacatctg agccaagccg tcctcgatcc cgtctatcgc cacggagagt
      781 ccaaactcat gctcagcgct gctcatatgg agtcgttgcc gactttcttc gcacgcattc
      841 ccgacccgcg ccgccgccac ggccgacgcc atccgctgcc ggcggtgctg gccatcgcca
      901 gcgccgcgac cctgtgtggg atgcgcggct acaaggcgat cgccgaatgg gcgcaaagcc
      961 tgggacaaag cgcgcgcgcg cgctttcgct gccgctaccg cgacggccgc tacgagattc
     1021 cgagcgaatc gatcatccgc gacgtgttga tccgggtcgg cccggagcac ctggatcgtg
     1081 ccctgcaaga ctggaatgcc gcctatgggg tgcaggatca agccttggcc atcgacggca
     1141 agacgctgcg caatgccatc gatcccgagg gccggcaggt gcatgtgatg agtgtcatcg
     1201 gccatcaaag cggcaccgcc cacgcccaaa aaaagtcggc actctgcccg ccgctgacga
     1261 acaggccaaa caaaccaacg agatcggaac cttcatcccg ctcgtcgagt ccatcgacct
     1321 cgccggtaaa accctcactg ccgacgcgct gctgacccaa cgcgcgctcg ccacctacct
     1381 cgtcgagaaa cgtcaggccc actacctgtt caccgtcaag ggcaaccagc cccggctgct
     1441 ggccgatctg caactggcct tcgcgcaacg cggcaagccg gagttccagg aacccctgga
     1501 cctggcccac ggccgcatcg agacgcgcgc gatctggacc tccacccagt tcaacgatta
     1561 tctcgacttt ccccatgtcg gacaggccta cctgatcgag cgctcggtgg tgcacaagaa
     1621 aagcggcaag agcaccaccg aactcgtcta tggcgtcacc agccattccg cggccagcgc
     1681 caatccgatg caggtgctcg ccctcaaccg caaccactgg gccatcgaga acggctgcca
     1741 ctgggtgctc gactggaact gggacgagga tcgcagctgt atccgcaccg gcaacggccc
     1801 cgaaaacatc accaggctgc gccgcttcgc catcagcgtg atcaagatca agagtcgcca
     1861 aagcgtcgcc gccaccttgc gcaaactgca acgcaatgtg cgcctggcct tcgactatct
     1921 ctgcatgact cgaaactcca cccgccgcgc tgcggctatc ggtgacatgg tcaggagaac
     1981 aaattagccc tggccggccg gccggcctac gttgccgcgc aatgggccag cctgcagcag
     2041 gccgtcgcct ggattggcgg cgccggcggc ctggccgtgc tcgcgcaccc gcgccgctac
     2101 acgctgagcg cggcggcgcg ccgacgcctg ctgtccttct ttcgcgaagc cggtggcgac
     2161 ggcatcgagg tctgctgcgc gacctcgacc gccgcagaaa tcgaaaccag cgcacgcgaa
     2221 gcgcgcgagc acgg
//
LOCUS       DASWIY010000194         8122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_106657, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000194 DASWIY010000000
VERSION     DASWIY010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106657"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VGA59_07105"
     CDS             complement(<1..407)
                     /locus_tag="VGA59_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9719486.1"
                     /translation="MPAIRTFHLSDVARMAGVSTASVSRVLNHPDKVSERIRVKVTQA
                     MEELGYVRDGAARALASRRSYSMGSVVPTIGIGIFASGVERLQQRLQQFGYQLLIATT
                     QYDASKEMEAVRALIERGVDGVQLVGQQHDPRI"
     gene            699..2006
                     /locus_tag="VGA59_07110"
     CDS             699..2006
                     /locus_tag="VGA59_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9719487.1"
                     /translation="MSARKLLKSTLTSLALSGTILAAGLLTPAQAEVTELKIGYMKHP
                     IQDASVEIMDKWAKENGIKLVKIPMAYSIFMEKVTATLTSGGDQFDIIWHNDDWGALW
                     LKWLETSDDIAGMDQVARHPLEAFLDEDGNVSVVPMTHTVGTFFYRTDLISPDEVPTT
                     WEELVEVSKRLQEEDKVKWGYVGGMAMNNTWFSLWWTMWSNKCDIFMPIWERDNDKLA
                     ENGWTPALTEPCTKEYVDFWWDAMNDHKISPEAMTAYTRDEANAIFMAGDAAFTLVDS
                     THYGDFNNPDKSRIAGKIGMAPFPMGPRGEVPFSWNEIWGWGIPKGVPEDRKAAAKEM
                     LGAMLLDFEGQVEMWNKTGGPPPNSALWEELEKNDEVFRQLKHAVFDQKHGTHAAYYF
                     PNWPAVHKAYSDVMIGALKGDRDDIPAALEAGMEQVSKAASGM"
     gene            2037..3002
                     /locus_tag="VGA59_07115"
     CDS             2037..3002
                     /locus_tag="VGA59_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9719488.1"
                     /translation="MTARHHAEGAPGLSPAPGLPSRRSARPWYHVNEKTRFTLLLLAP
                     SATLLVLFQIMPIIIGANASFRDWRLYDPQKTWVGLQHYIYILTDPVFLTLVLPNTLL
                     LMVLSVSIGLCLGLALAHLMNQHFVGHTVIQTIILLPLMIAPVIASMMMRWIFNDQFG
                     VVAAVMEALGLGSIAWLSDRWPSFAIIVLTDVWLWTPWFTIILLAGLRSLPKEPYEAA
                     AIDAAGKWRVFTHITLPMMRSVMAVCIVIRSIDAFRTFDQVWVITGGGPARQTEVFSI
                     YAYTEAFVFLNFARGTAAAIIGAMIIGTLGWGLYKMLNRFMEVSR"
     gene            2999..3856
                     /locus_tag="VGA59_07120"
     CDS             2999..3856
                     /locus_tag="VGA59_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9719489.1"
                     /translation="MTAATGAYPAPRRASFLDALAPGGRRVFFLLCITAVLLLFAFPV
                     FWLVLTSLRPAWGVFYVHRGLEFTLSNYSQVLEQEVVVNAFINSFVIATLATFFSLTV
                     TVTSGYMLSRFTGKLANAWFGTIYIFRTIPYITWVLPLYFVTQWWGIFDTYLGLLLPH
                     IAVHICFFSWLMKGFFDGIDPSMESAALIDGCTRWGAFLRVALPSAIPGIAALSVLCW
                     LWTWNEFLFALILTSHNTPMITVVMAQFVSELGTEWNLMSATAVMAMGPALIVAIFGQ
                     KYVVRGLKV"
     gene            3895..5055
                     /gene="ugpC"
                     /locus_tag="VGA59_07125"
     CDS             3895..5055
                     /gene="ugpC"
                     /locus_tag="VGA59_07125"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX9719490.1"
                     /translation="MAEVKLTKVNKYYGNVLAVHEVDLTVADEEFVVLVGPSGCGKST
                     TLRMVAGLEEISGGTIDIGGRVVNDLPPRDRDIAMVFQNYALYQHMTVYDNLAFGLRN
                     RKVPEDQIRKEIDRATAILGIAPLMGRKPKQLSGGQQQRVALGRCIVRNPAVFLFDEP
                     LSNLDAKLRAQMRIELKELRTRVATTSIYVTHDQVEAMTLGDRIVVMKDGVVQQTGTP
                     LQLYRTPANRFVASFLGSPAMNFITVKVSQKDGGLALTGFGIDIAVPDAMAARLAGHV
                     GQSVVIGLRPEHIVLGDDGGGAVIRLDGRVVLTEQLGAQQLVELRVGDHDVMAAGVDP
                     ELNLKSGESGRFFISLERVHIFAPGENGAVVWSAGSGKLEAPAPQVSETTSV"
     gene            5071..5523
                     /locus_tag="VGA59_07130"
     CDS             5071..5523
                     /locus_tag="VGA59_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9719491.1"
                     /translation="MSDFYVKPGDEVRFSKTVGESDVYLFAGITGDLSYNHVNEEFMR
                     KSSYGRRIAHGALMVGFMSTASTMMIDFARGTDGDETPVSLGYDRIRFLGPVYIGDTI
                     TVTYRITAVDAARRRSTSEITITNQKGDLVTVGSHVLKWVANAAQAAE"
     gene            complement(5550..6323)
                     /locus_tag="VGA59_07135"
     CDS             complement(5550..6323)
                     /locus_tag="VGA59_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9719492.1"
                     /translation="MDTQILVSRKADGILHLQLNRPEARNALSRALIGALADALRAAS
                     ADEAVRVVLLGGGERQFCAGADIKEFVAEGGAVLENTRRTEDWSAIERFAKPLVAAVE
                     GYAFGGGFELVLLADIVVAAENARFGFPEINLGLFPGDGGTQRATRVAGKALVMKMIM
                     TGEPIDAAAAQASGLVSELAPAGSVAARAEELAGLLATKPPVALRLAKASILAAYDVP
                     LSAGLVLERQLVARAFATEDLVEGVTAFRDKRAPRFKGR"
     gene            6413..7606
                     /locus_tag="VGA59_07140"
     CDS             6413..7606
                     /locus_tag="VGA59_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9719493.1"
                     /translation="MNIVSFCHFLQGPAATQYLGDMGANVTKIEPRQGAYERHWAGAD
                     RAKVGGISAFFLGSNRNARSLAIDLKHPRAREVIYRLIDNSHAVVENFRPGTLARLGF
                     GYEAVKARKADIIYASASGFGASGPYADRPGQDLLIQAMSGLVAATGGRRGPTAAGCA
                     AADQHGGALLALGILGAYSKWQRTGEGTLVEANLLAAGVDLQTESLVTYYASGRGKEV
                     FDRDDRLATWFHEAPYGVYKIADGHMVLSLNATDKLADALQSEALRAFVGRNAYDERD
                     AYAQAMAEVLAPLSFSEVAAKLDAHKLWYARVEDFDDLRRNPQLLHNEMFREVPVNDA
                     TVTLLNHPIRYDGKVPEFKGFALQPGEHTRDVLGDNGLSADEIEALLKDGVVFAPADE
                     MVPAE"
     gene            7668..>8122
                     /locus_tag="VGA59_07145"
     CDS             7668..>8122
                     /locus_tag="VGA59_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9719494.1"
                     /translation="MLNLLEGLKVVSFNHFLLGPVGMQALADMGADVISVEPLEGAFQ
                     RQFGGGVGVFVDGQASLFLTGNRNKRSIVLDLKSEKGREAARKLAESADVVSENFRPG
                     VMDKLGLGYETLKERNPGLIYAAASGFGADGPYARRPGQDLLIQAMSGLA"
BASE COUNT         1425 a         2638 c         2638 g         1421 t
ORIGIN      
        1 tagatgcgcg ggtcgtgctg ctggcccacc agctgcaccc cgtccacccc gcgctcgatc
       61 agggcgcgca ccgcctccat ctccttcgag gcatcatact gggtggttgc gatcagcagc
      121 tgatagccga actgctgcag gcgctgctgc aggcgctcga cgccagaggc aaaaatgccg
      181 atgccgatgg tcggcaccac actgcccatg gaataggagc ggcgcgacgc cagggcgcgc
      241 gcggccccat cgcgcacgta gcccaactcc tccatggcct gcgtgacctt gacgcgaatg
      301 cgctcgctca ccttgtccgg atgattgagg acgcgcgata ccgatgccgt cgaaacaccc
      361 gccatcctgg ccacgtcgct gaggtgaaag gttcgaattg ccggcacttt ctttcttctg
      421 acggattatg ctcttatacc tcagcgcagg ccgcggggcg aatgattaaa gcaccgcgca
      481 tccatcgccc cgcgcaacgt gtcccgcagc cttgccgccc accggtggcg aaaaatgccg
      541 ggagcgcctg cttgacagcg agtccatcgc gtgtactatg taaacgctta caaataggga
      601 ccgggcgagg atccggattc aaggtgaatc gacgggacta caacaagaac tcagaacaga
      661 gttgacctat tcagtccttg aacaacggga ggaacggcat gtccgccaga aagctgctga
      721 agagcacact tactagcttg gcgttgagcg gcactatact tgccgctggc ttgctcacac
      781 cggcgcaagc cgaagtgacc gagctgaaaa tcggctacat gaagcatccc atccaggatg
      841 ccagcgtcga gatcatggat aaatgggcca aggagaacgg catcaagctg gtcaagattc
      901 cgatggccta ttcgatcttc atggaaaagg tgaccgccac cttgacctcc ggcggcgacc
      961 agttcgacat catctggcat aacgacgact ggggtgcgct gtggctcaag tggttggaga
     1021 cctcagacga catcgcaggc atggaccagg tggcgcggca tccgctggaa gccttcctcg
     1081 acgaagacgg caatgtcagc gtggtgccga tgacccacac cgtgggaacg ttcttctacc
     1141 gcaccgacct gatctcgcct gacgaggtgc cgaccacctg ggaggagctt gtcgaagtca
     1201 gcaagcggct gcaggaggag gacaaggtga agtggggcta tgtcggtggc atggccatga
     1261 acaacacctg gttctcgctg tggtggacga tgtggtccaa caagtgcgac atcttcatgc
     1321 cgatctggga gcgcgacaac gacaagctcg ccgagaacgg ctggacgccc gccctgacgg
     1381 agccctgcac caaggaatac gtcgacttct ggtgggatgc gatgaacgac cacaagatca
     1441 gcccggaggc gatgacggcc tatacccgcg acgaggcgaa cgccatcttc atggccggcg
     1501 atgccgcctt cacgctcgtc gactccaccc attacggcga tttcaacaac ccggataagt
     1561 cgcgcatcgc cggcaaaatc ggcatggcac cctttcccat gggtccgcgc ggcgaggtgc
     1621 cgttctcctg gaacgagatc tggggctggg gcatccccaa gggcgtgccg gaggaccgca
     1681 aggcggcggc caaggagatg ctcggcgcca tgctgctcga cttcgagggc caggtggaaa
     1741 tgtggaacaa gaccggcggc ccgccgccga actcggcact gtgggaggaa ctcgagaaga
     1801 atgacgaggt gttccgccag ctgaagcatg cggtgttcga tcagaagcat ggcacgcatg
     1861 cggcctacta cttccccaac tggccggccg tccacaaggc ctattcggac gtcatgatcg
     1921 gcgccctgaa gggtgaccgc gacgacatcc cggcagccct ggaagccggc atggagcagg
     1981 tcagcaaggc tgcctccggc atgtaaatcc ggcagcagca ggcaaagcag gctatcgtga
     2041 ctgcccgtca ccatgccgaa ggcgccccgg ggctgagtcc agccccgggg ctgccgtccc
     2101 gccgttctgc gcggccgtgg taccatgtca acgagaagac gcgcttcacg ctcctcctgc
     2161 tggcgccctc ggcgacgctg ctggtcctgt tccagatcat gcccattatc atcggagcca
     2221 atgccagctt tcgcgactgg aggctgtatg atccgcaaaa gacctgggtc gggctgcagc
     2281 attacatcta cattctcacc gatccggtgt tcctgacgct ggtgctgccc aataccctgc
     2341 tgctgatggt gttgtccgtc agcatcgggc tttgcctggg gctggcgctg gcgcatctga
     2401 tgaaccagca ctttgtcggc cacaccgtca tccagacgat catcctgttg ccgctgatga
     2461 tagctccggt gattgcctcg atgatgatgc ggtggatttt caacgaccag ttcggcgtcg
     2521 tcgccgccgt catggaggcg ctcgggctgg gatcgatcgc ctggctgtcc gaccgctggc
     2581 cgagcttcgc catcatcgtg ctgaccgacg tgtggctgtg gacgccgtgg ttcaccatca
     2641 ttctgctggc gggcctgcgc agcctgccga aggagccgta cgaggcggcc gcgatcgatg
     2701 cggctggcaa gtggcgggtg ttcacccaca tcaccctgcc gatgatgcgc tcggtgatgg
     2761 cggtgtgcat cgtgatccgc tccatcgacg cgtttcgcac cttcgaccag gtctgggtca
     2821 tcaccggcgg cggcccggcc cggcagaccg aggttttcag catctacgcc tatacggaag
     2881 ccttcgtgtt cctgaacttc gcgcgcggca ccgcggcggc gatcatcggc gccatgatca
     2941 tcggcacgct cggctggggc ctctacaaga tgctcaaccg cttcatggag gtgtcgcgat
     3001 gacggctgct accggcgcat atcctgcgcc gcgccgcgcc agctttctcg atgcgctggc
     3061 gccgggcggc aggcgcgtgt tcttcctgct gtgcatcacc gccgtgctgc tgcttttcgc
     3121 ctttccggtg ttctggctgg tgctgacctc gctcaggccg gcatgggggg tgttctacgt
     3181 gcaccgcggg ctggagttca ccctttcgaa ttattcccag gtgctggagc aggaggtggt
     3241 ggtgaacgcc ttcatcaaca gcttcgtcat cgccacgctg gcgaccttct tttccctgac
     3301 cgtcacggtg accagcggct acatgctgtc gcgcttcacc ggcaagctgg ccaacgcctg
     3361 gttcggcacc atctatatct tccgcaccat cccctacatc acctgggtgc tgccgctgta
     3421 tttcgtcacc cagtggtggg ggatcttcga cacctatctg ggcctgctgc tgccccacat
     3481 agccgtccac atctgcttct tctcgtggct gatgaagggc ttcttcgacg gcatcgaccc
     3541 gtcgatggag tcggcggcac tgatcgacgg ctgcacccgc tggggcgcct ttctgcgtgt
     3601 cgcgctgccc tcggccatac ccgggatcgc ggccttgagc gtgctgtgct ggctgtggac
     3661 gtggaacgag ttcctgttcg ccctgatcct gaccagccac aacacgccga tgatcaccgt
     3721 ggtcatggcc cagttcgtgt cggagctcgg caccgaatgg aacctgatga gcgccaccgc
     3781 ggtgatggcg atggggccgg ccctcatcgt cgccatcttc ggacagaaat acgtggtgcg
     3841 cgggctgaag gtctgaccgc gaaccgacga gcttgaagga agcggacacc cgccatggct
     3901 gaagtgaagc tgacaaaggt caacaagtac tacggcaatg tgctcgccgt gcatgaggtc
     3961 gacctcacgg tggccgatga ggagttcgtg gtgctggtcg ggccgtccgg ctgcggcaag
     4021 tcgaccaccc tgcgcatggt cgccgggctc gaggaaatca gcggcggcac catcgatatc
     4081 ggcggccggg tggtgaacga cctgccaccg cgcgaccgcg atattgccat ggtgttccag
     4141 aattacgccc tctaccagca catgacggtg tacgacaatc tggccttcgg gcttcgcaac
     4201 cggaaagtgc cggaagacca gatccgcaag gaaatcgacc gcgccaccgc catcctcggc
     4261 atcgccccgc tgatggggcg caagcccaag cagctgtctg gcgggcagca gcagcgcgtg
     4321 gcgctgggcc gctgcatcgt gcgcaacccg gcagtgttcc tgttcgacga gcccctgtcc
     4381 aacctcgacg ccaagctgcg ggcccagatg cgcatcgagc tgaaggagct gcgcacccgg
     4441 gtcgccacca cgtccatcta tgtcacccac gaccaggtcg aggcgatgac gctgggcgat
     4501 cgtatcgtgg tgatgaagga cggggtggtg cagcagaccg gcacgccgct gcagctctac
     4561 cgcacgccgg ccaaccgctt cgtcgccagc ttcctcggct ctccggcgat gaacttcatc
     4621 accgtgaagg tgagccagaa ggacggcggc ctggcgctga ccggtttcgg catcgacatc
     4681 gccgtgccgg acgcgatggc ggcaaggctt gccggccacg tcgggcagtc cgtggtcatt
     4741 gggctgcggc cggagcatat cgtgctgggc gatgacggtg gcggcgcggt gatccggctg
     4801 gacggccgcg tcgtgctgac cgagcagctc ggcgcacagc agctggtcga gttgcgggtg
     4861 ggcgaccatg acgtgatggc cgccggcgtc gaccccgaac tcaatctgaa aagcggcgag
     4921 tcggggcgct tcttcatcag cctcgagcgg gtgcacattt tcgccccggg cgagaacggt
     4981 gccgtcgtgt ggtcggccgg cagcggcaag ctcgaggcgc cggcgcccca ggtgtccgag
     5041 acaacatccg tctgagcggg agaaacagca atgagcgact tttacgtgaa gcccggcgac
     5101 gaggtgcgct tttcgaagac cgtgggggag agcgacgtct atctgttcgc cggcatcacc
     5161 ggcgatttgt cctacaacca cgtcaacgag gagttcatgc gcaagtcgag ctacggccgg
     5221 cgcattgccc atggcgcgct gatggtgggc ttcatgtcca ccgcttccac catgatgatc
     5281 gactttgcca ggggcaccga cggcgacgag accccggtgt cgctgggcta cgacaggatc
     5341 cgcttcctcg gcccggtgta catcggcgac accatcaccg tcacctatcg cattaccgcg
     5401 gtggatgcgg cgcgccgccg ctccacctcc gagatcacca tcaccaacca gaagggcgac
     5461 ctggtgacgg tcggcagcca tgtcctgaaa tgggtcgcca atgccgccca ggcggcagag
     5521 tgatcctcaa ggcgcagccc atggtctgtt cagcgtccct tgaaccgcgg ggcgcgcttg
     5581 tccctgaacg cggtcacgcc ctcgaccagg tcctcggtgg cgaaggcacg cgcgacaagc
     5641 tggcgctcca gcaccagccc ggcgctgagc ggcacgtcgt atgccgccag gatggaggcc
     5701 ttggcgaggc gcagcgccac cggcggcttg gtggccagca gcccggccag ttcctccgcc
     5761 cttgccgcca cgctccccgc gggcgccagc tcggagacca gtccggacgc ctgggcggcc
     5821 gcggcatcga tcggctcgcc ggtcatgatc atcttcatca ccagcgcctt gccggcgacg
     5881 cgggtggcgc gctgggtgcc gccgtcgccg ggaaacaacc ccagattgat ctccgggaag
     5941 ccgaagcggg cattttccgc cgccaccacg atgtcggcca gcagcaccag ctcgaagccg
     6001 ccgccgaagg catagccctc gaccgccgcc accagcggct tggcgaagcg ctcgatggcc
     6061 gaccagtcct cggtgcgcct ggtgttctcc agcaccgcac cgccctcggc tacgaactcc
     6121 ttgatgtcgg ccccggcgca gaactgccgc tcgccgccgc ccagcagcac cacgcgcacc
     6181 gcctcgtccg cgctggccgc gcgcagcgcg tccgccagcg cgccgatcag cgcccggctc
     6241 agggcgttgc gcgcctcggg ccggttcagc tgcagatgca ggatgccgtc ggccttgcgg
     6301 ctgaccagaa tttgcgtgtc cacggggttc ctttcttgag ggggcgccgg gaaacgttgt
     6361 gtaaccgctt gcttcagcga ttcaaagggg gtgcctcgtg cttcttgacg gtatgaacat
     6421 cgtcagcttc tgccattttc tgcaaggccc ggcggcgacc cagtatctgg gcgacatggg
     6481 cgccaacgtg acaaagatcg agccgcgcca gggcgcctat gagcgccact gggccggcgc
     6541 cgaccgggcc aaggtcggcg gaatcagcgc cttctttctc ggctccaatc gcaatgcccg
     6601 cagcctcgcc atcgatctga agcatcccag ggcccgggag gtgatctacc ggctgatcga
     6661 caacagccat gcggtggtgg agaacttccg ccccggtacg ctcgcacgtc tgggcttcgg
     6721 ctatgaggcg gtgaaggcgc gcaaagccga catcatctat gcctcggcct cgggcttcgg
     6781 ggcgagcggc ccctatgccg accggccggg ccaggatttg ctgatccagg cgatgagcgg
     6841 cctggtcgcg gcgaccggcg ggcggcgcgg gccgactgcg gcgggctgcg cggcggccga
     6901 tcagcatggc ggcgcgctgc tggcgctcgg catcctcggc gcctattcga agtggcagcg
     6961 caccggggag ggcactttgg tggaggccaa tctgctggcg gcgggcgtcg acctgcagac
     7021 cgaatccctg gtgacctact acgccagcgg ccgcggcaag gaggtgttcg atcgcgacga
     7081 ccgactggcc acctggttcc acgaggcgcc atacggggtc tacaagatag ccgacggcca
     7141 catggtgctg tcgctgaacg ccaccgacaa gctggcggac gccctgcaaa gcgaggcgct
     7201 gcgcgccttc gtcggccgca acgcctatga cgagcgcgac gcctacgccc aggcgatggc
     7261 cgaggtgctg gcgccgctga gcttctccga agttgccgcc aagctggatg cgcacaagct
     7321 gtggtacgcc cgggtcgagg acttcgacga cctgcggcgc aatccccagc tgctgcacaa
     7381 cgagatgttc cgggaagtgc cggtcaacga tgccacggtg acgctgctca accatccgat
     7441 ccgctacgac ggcaaggtgc cggagttcaa gggcttcgcc ctgcagccgg gcgagcacac
     7501 gcgcgacgtt ctgggcgaca acggcctgtc ggccgacgag atcgaggcgc tgttgaagga
     7561 cggtgtcgtc ttcgcgccgg ccgacgagat ggtgccggcc gagtgagcga gggctggcct
     7621 ggacatatca cgcgtgagat ggtcgtccct gtggagtgcg cgccgacatg ctgaacttgc
     7681 tcgaggggct gaaggtcgtc agcttcaatc atttcctgct cggtcccgtg ggcatgcagg
     7741 cgcttgccga catgggggcc gacgtgatct cggtcgaacc gctggaaggc gcgttccagc
     7801 gccagttcgg cggcggcgtc ggggtgttcg tcgatggcca ggcgtcgctg ttcctcacgg
     7861 gaaaccgcaa caagcgcagt atcgtcctcg acctgaagtc ggaaaagggc cgcgaggcgg
     7921 cgcgcaagct ggccgagagc gccgatgttg tgagcgagaa cttccgcccc ggcgtgatgg
     7981 acaagctggg gctcggctat gagacgctaa aagagcgcaa cccggggctc atttatgccg
     8041 ccgcctccgg cttcggcgcc gacggcccct acgcaagaag gcccggccag gacctgctga
     8101 tccaggccat gagcggtctg gc
//
LOCUS       DASWIY010000195         6133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1215658, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000195 DASWIY010000000
VERSION     DASWIY010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215658"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VGA59_07150"
     CDS             complement(<1..953)
                     /locus_tag="VGA59_07150"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9719495.1"
                     /translation="MIFDYRIHRRTSGLLLCLATLLCLPVTQVWAQGKQQQAAAPHQR
                     AQPGRSVPQDATAHMEQLNADKATPPLARGARGAAVVRVQVLLDRAWFSPGEIDGIFG
                     TNMAKSVAAFQASRALPTTGRIDGATWTALQDGQGPVFATYALTEQDIAGPYTKLPRD
                     PMEQGKLPALGYQSLQEALAERFHMSPKLLAEFNRGRRLQPGTLVVVADTAGSNAPPA
                     QAALLRIDKSEKMLYVLDEDDRVLAAFPVSIGGAGDRLPLGRLKITSEVKNPPFTYDP
                     ALLKSAKPTAVKTKLPPGPNNPVGVMWLGLNKPHWGIHGTS"
     gene            1473..3563
                     /locus_tag="VGA59_07155"
     CDS             1473..3563
                     /locus_tag="VGA59_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9719496.1"
                     /translation="MEFQSDRNSMNDDDIARTALQHLSELRRALSLERAGASEGEVLD
                     TLLRAARLYAGSDARIALILVDEDNARLKFVASDGLSEQYAQTVHHTPIGVELVPCVR
                     AALSGEMVIVDDITRHAGCAQYLSFAEQQSIKACWSFPVRAQRDKVLGALAACFPAPQ
                     KADPYRIEMLEFVAQCAALTIERYAETEERRKSEAQLRASEARYRSLTDVLTSVVWTT
                     DAQGRFVAPQEAWRKYTGQPWEQQQGFGWFQAVHPDDKERVLASVLRAHEANDYYRAD
                     GRLWHEASQTYRYCEARGTPIVGANGEVTEWIGSCVDVDDQKQAEAALQLADRRKDEF
                     IAVLAHELRNPMAPIRNAIHILKARVAGDEQLSRVSTIMDRQAGKMARLLDDLLDVSR
                     IAQDKLELRKKRVSLNSILESAIETSRPLLEQQGHHFELHAPADPIYLEADAARLAQA
                     FSNLLNNAAKYTDHGGRIGMTVRQEGKAAVVSVKDNGIGIEADMLPKLFNRFSQAAPA
                     LERAQGGLGIGLSLVLGLVEMHGGTVRGSSDGAGRGSEFVVRLPVGPARAAAVAHQEP
                     EQPGAARMRILVADDNQDTAETLSVLLGMMGHEVREAADGQEAVDLATSFRPAVALLD
                     IGMPKVNGYDAATRIRATLPGVLLVAMTGWGQEEDMRKATAAGFDHHLVKPVNPQQLE
                     RLLAALSPAQRQGS"
     gene            3690..3944
                     /locus_tag="VGA59_07160"
     CDS             3690..3944
                     /locus_tag="VGA59_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719497.1"
                     /translation="MKAFANKFVPWLTAALLCAGAILVATDAGLDTRRRYMAGAALLV
                     LLIVCALRLVLKAMAATRSNVIEHAPEMSSLAFPPSSFRK"
     gene            complement(3991..4689)
                     /locus_tag="VGA59_07165"
     CDS             complement(3991..4689)
                     /locus_tag="VGA59_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9719498.1"
                     /translation="MATRVVMITGAAGNLGQAVAQAFAQAGDTLVLAGRTRESLVRAY
                     GEETPQRLFAVADLLDPAQLDAAVRLAVQRLGGIDVLCNIAGGFQMGPAVHETTDESW
                     DLMMDLNARTVMHTARAVVPNMLSRGGGKIVNVGANAAMKGAAHMGAYVASKSSVLRL
                     TETMAAELREGGINVNCVMPSIIDTPENRAAMPGADPARWVKPQQLAQVIAFLASDAA
                     SALHGACIPVVGLA"
     gene            complement(4691..5605)
                     /gene="phhA"
                     /locus_tag="VGA59_07170"
     CDS             complement(4691..5605)
                     /gene="phhA"
                     /locus_tag="VGA59_07170"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEX9719499.1"
                     /translation="MKNRSEPDTAALQAAQLHGLAAGESSRPANPDWTIPQGWENYTA
                     EEHGVWKTLFERQTALLPGRACDEFVHGMTDLPMGAHEIPDFRRLSDALMERTGWQVV
                     AVPGLVPDEVFFEHLAHRRFPAGQFIRKPHQLDYLQEPDVFHDVFGHVPLLMNPVIAD
                     YIQAYGQGGLRALQLGKLPCLARVYWYTVEFGLVRQPEGLRIYGAGIASSRAETVFSI
                     DDPSPNRIGFELERVMRTLYRIDDFQETYFVLDSLDELLGLAHIDFAPLYERVGAQSE
                     LQPGTVLPTDNVLHQGSGNYHAQHRGGA"
     gene            complement(5684..>6133)
                     /locus_tag="VGA59_07175"
     CDS             complement(5684..>6133)
                     /locus_tag="VGA59_07175"
                     /inference="COORDINATES: protein motif:HMM:NF021541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2272 domain-containing protein"
                     /protein_id="HEX9719500.1"
                     /translation="DYVDKAVLTSSAEVRGEFTEYAYRACDVATTRPRQGDMICYTRG
                     SAAAIDSHAKLLAHLELRRSVGDSGLVPMHCELVTSSDENGNAKIETIGGNVFQSVTL
                     RKMTLNATKTLSRTYFHSRRPPVCTQGKSCSGNLSWKRWVVLLQFRN"
BASE COUNT         1048 a         1991 c         2015 g         1079 t
ORIGIN      
        1 tcactcgtgc cgtggatgcc ccagtgcggt ttgttcaggc ccagccacat cacgcccacc
       61 gggttgttcg gccccggagg gagcttggtc ttcacggccg taggcttcgc ggatttcagc
      121 aacgcggggt cgtaggtgaa aggcgggttc ttcacttcgc tggtgatctt gagccgcccc
      181 agcggcaggc gatcgcccgc gccgcctatg ctcaccggga acgcggccag cacccggtcg
      241 tcttcgtcca gcacgtagag catcttctcg gatttgtcga tgcgcagcag ggcggcttgc
      301 gcgggcggcg cgttcgatcc ggccgtgtcg gccaccacga cgagcgtacc gggttggagc
      361 cggcggccgc gattgaactc ggccagcagc tttgggctca tgtggaagcg ctcggccagg
      421 gcctcctgaa gcgactggta ccccagggca ggcagcttgc cctgctccat cgggtcgcgt
      481 gggagcttgg tgtaagggcc cgcgatgtcc tgctcggtca atgcgtaggt ggcgaaaacc
      541 ggtccctgcc catcctgcaa ggcggtccag gtggcgccat cgatgcggcc ggtcgtcggc
      601 agcgcacggg acgcctggaa ggccgccacc gacttggcca tgttggttcc gaaaatgccg
      661 tcgatctccc ccggcgagaa ccatgcccgg tccaggagca cctggacgcg caccacggcc
      721 gccccgcggg caccgcgtgc cagcggcggg gtggccttgt ccgcattcag ctgctccatg
      781 tgcgcggttg cgtcctgggg caccgaccgc cccggttgag cccgctgatg gggagctgcg
      841 gcttgctgct gctttccttg cgcccacact tgggtgacgg gcaggcatag cagcgtggca
      901 agacacagca gcaggcccga ggtacgacga tgaatcctgt agtcgaaaat catgtggggc
      961 cttcgggaga acgtttgccc taccctagcg tggctggggc gcacacgctg tcggtcacag
     1021 ccgacggttc gagtcggcgg cagccgatca aacggctctg cgggcagcga cccttcagcg
     1081 acgcacctgt caccggcgca cgcgcgccct ggaagccatg caggaaagac gcggcgctcg
     1141 tgcgcgcaga aaaaaccccc gaagcttgcc tgccgcgggg gtgtgaggga ccgaccctca
     1201 agccctacta ggagagattg cttgcaccgt cactgcggca atagtgactg cttcgcgctc
     1261 gcagcgcttg agaaacatca accagcccgg cctgaagagg ctgctgttac acgcggcact
     1321 tgccgccggg ttttcgcccc cggatacgcg agtggccgtt cggcagccgt ggccgcgggt
     1381 tgcgcttgtg ttgatctaaa gcattagatc cagctctttc gcttgatgcc tgtcaaggaa
     1441 gctcttgcgt agcctctttc taatgaacca cgatggaatt ccagtcggat cggaactcga
     1501 tgaacgacga tgacatagcg cgtacagcgc tacagcacct ttccgagctc aggcgcgccc
     1561 tctccctcga aagagccggc gcctccgagg gcgaggtgct ggacaccctc ctgcgtgccg
     1621 cccggctcta tgcgggcagc gatgcccgca ttgcgctgat cctggtcgat gaagacaatg
     1681 cccgcttgaa gtttgtcgct tcggatggcc tgtcggagca gtacgcccag accgtgcacc
     1741 atacgcccat cggagtcgag ttggtgccct gtgtccgggc ggcgctttcc ggagagatgg
     1801 tcattgtcga tgacatcact cggcatgccg ggtgcgctca atacctgagt ttcgccgagc
     1861 agcagtccat caaggcttgc tggtcgtttc ctgtccgcgc gcagagggac aaagtactgg
     1921 gcgcgctcgc ggcatgtttc cccgccccgc aaaaggccga tccttaccgc atcgaaatgc
     1981 tggagttcgt ggcgcaatgc gcggccctga cgatcgagcg gtatgccgaa accgaagagc
     2041 ggcgcaagtc ggaagcgcaa ctgcgtgcga gcgaggcgcg ctaccggtcg ctcaccgatg
     2101 tgctgacttc ggtggtctgg accaccgatg cgcagggccg cttcgttgcg ccgcaggagg
     2161 cctggcgcaa atataccggc cagccctggg agcagcagca gggcttcggc tggttccagg
     2221 cggttcaccc cgacgacaag gagagggttc tggcatcggt gctcagggcg cacgaggcca
     2281 atgactacta tcgggcggac gggcggcttt ggcacgaggc cagccagacc taccggtact
     2341 gtgaagctcg cggcacgccg atcgttggcg ccaacggtga ggtgaccgaa tggatcggga
     2401 gctgcgtgga cgtcgatgac cagaagcagg ccgaggcggc gctccagctg gccgaccggc
     2461 gcaaggacga gttcattgcc gtgctggcgc acgagttgag gaacccgatg gcgccgatcc
     2521 gcaacgccat ccatattctc aaggccaggg tggccggcga cgagcagctt tccagagtca
     2581 gcaccatcat ggaccgccag gcgggcaaga tggcgcggct gctggacgac ttgctggatg
     2641 tcagccgcat cgcccaggac aagctggagt tgcgcaagaa gcgcgtgagc ctgaactcca
     2701 ttctcgaaag cgcgatcgag accagccgtc cgctgctcga gcagcagggg catcatttcg
     2761 aactgcacgc gccagccgac ccgatctacc tcgaggccga cgccgctcgc ctggcacagg
     2821 ccttctcgaa cctgttgaac aacgcggcga agtacaccga ccatggcggt cggatcggca
     2881 tgacggttcg gcaggaaggc aaggcggcgg ttgtttccgt gaaggacaac ggcatcggca
     2941 tagaggccga catgttgccc aagctgttca acaggttctc gcaggccgcc ccggcactgg
     3001 agcgcgcgca aggcggcctt ggaatcgggc tctccctggt tctcgggctg gtggaaatgc
     3061 acgggggcac ggtgcgcggc tccagcgacg gagcgggtcg tggcagcgag ttcgtggtcc
     3121 gccttccggt cgggccggcg cgagcggcag ccgtggctca ccaggagccg gaacaacccg
     3181 gcgcggcacg catgaggatc ctggtggccg acgacaacca ggacaccgcg gaaacgctgt
     3241 ccgtgctcct gggaatgatg ggtcacgagg tgcgcgaggc tgccgacgga caagaggccg
     3301 tggacctggc caccagcttt cgcccggccg tggcgctgct cgacatcggc atgccgaaag
     3361 tcaacggcta cgatgccgcc acgcgcatac gggcgacgct gcctggcgtc ctgctcgtag
     3421 ccatgaccgg ctgggggcag gaagaagaca tgcgcaaggc caccgccgcc ggcttcgacc
     3481 atcatctggt caagcccgtg aatccgcaac agcttgaacg cctgctggcg gcgctgtcgc
     3541 cggcccaaag gcaaggcagt taggctggtg ccgactggcc ccgccttgtg tggcgggtgc
     3601 gcgacagaca ttcgccgcga agcgcggaag cctttgacgc ccgctggtac atcactagac
     3661 tggaccgatc aaggtttgcc tgttcgagga tgaaagcttt cgccaacaag ttcgtgccct
     3721 ggctcacagc cgctttgctt tgcgccggcg ccatactcgt ggccaccgat gcgggtctcg
     3781 acacccgacg caggtacatg gccggggccg cgctgctcgt gcttctcatc gtttgcgcgc
     3841 ttcgcctcgt tctcaaggcc atggcggcca cgcggtcgaa cgtgatcgaa cacgctcccg
     3901 agatgtcgtc actggccttt cccccatcct cttttcgcaa gtaggcgcag cccctgccgg
     3961 cgcgcttctc gccgccaccg gccatcgcgc tcaggcgagg cccaccaccg ggatgcaggc
     4021 cccatgcagc gcactggccg cgtccgaggc caggaaagca atgacctgtg ccagctgctg
     4081 cggcttcacc cagcgcgcgg ggtccgcacc cggcatcgcc gcgcggttct ccggcgtgtc
     4141 gatgatgctg ggcatcacgc aattgacatt gattcccccc tcccgcagtt cggccgccat
     4201 ggtctccgtc aggcgcagca ccgaggactt ggaggcaacg taagcgccca tgtgtgcggc
     4261 acccttcatg gcggcgttgg cgccgacatt gacgatcttg ccgccgccgc gggagagcat
     4321 gttggggaca acggcgcgcg ccgtgtgcat gacggtgcgc gcattgaggt ccatcatcag
     4381 gtcccaggat tcgtcggtgg tctcgtgcac cgccgggccc atctggaatc caccggcgat
     4441 gttgcacagc acgtcgatgc cgcccaggcg ttgcaccgcc aggcgaaccg ccgcatccag
     4501 ctgcgccgga tccagcaggt ccgccacggc gaaaaggcgc tgcggcgtct cttcgccgta
     4561 ggcccgcacc agcgactccc gggtgcggcc ggccagcacc agcgtatcac cggcttgcgc
     4621 gaatgcctgc gccacggcct gccccagatt gccggccgcg ccggtgatca tcacgacccg
     4681 agttgccatc tcaagctcct ccccgatgtt gcgcatggta gttgccgctc ccctggtgca
     4741 gcacgttgtc cgtcggcagc acggtacccg gctgcagttc gctttgcgcg ccgacgcgct
     4801 cgtacagcgg cgcgaaatcg atgtgcgcca gccccagcag ctcgtccagg gagtccagca
     4861 cgaagtaggt ctcctggaaa tcgtcgatcc ggtacagcgt gcgcatcacg cgctccagct
     4921 cgaagccgat ccggttgggc gatgggtcgt cgatgctgaa aaccgtctcg gcgcgcgacg
     4981 aggcgatgcc ggcgccatag atgcgcaatc cctcgggctg ccgcaccagc ccgaactcca
     5041 ccgtgtacca gtacacgcgg gcgaggcagg gcagtttgcc gagctgaagg gcccgcagcc
     5101 cgccctggcc gtaggcctgg atatagtcgg cgatcacggg attcatcagc aggggcacgt
     5161 ggccgaagac gtcgtggaac acatcgggtt cctgcaaata gtccagctgg tgcggtttgc
     5221 ggatgaattg gccggcggga aagcgccggt gcgcgaggtg ctcgaagaac acctcatcgg
     5281 gcaccagccc gggcacggcg accacctgcc agccggtgcg ttccatcagc gcgtccgaca
     5341 gacgccggaa gtccggaatc tcatgcgccc ccatcgggag atccgtcatg ccgtggacga
     5401 actcgtcgca ggcccgcccg ggcagcagtg cggtctggcg ctcgaacagg gttttccaga
     5461 cgccgtgctc ctcggccgtg tagttttccc acccctgcgg gatcgtccag tcgggattcg
     5521 cgggacggct cgattcgccg gctgccaatc cgtgcagctg cgccgcctgg agtgctgcgg
     5581 tatcgggttc ggaacgattt ttcatggtgc gctggatgtt gagatgctgg agggctcatg
     5641 gagttcaagg acggaccgct gacgcgttcg gctgcattca tcgtcaattc ctgaactgga
     5701 gcaggacaac ccaccgtttc caactgaggt tgccgctgca tgatttgccc tgggtgcaaa
     5761 cgggcggacg gcgtgagtga aagtacgtcc tgctcaaagt cttcgtggcg ttgagagtca
     5821 tctttcgcag cgtgacggac tggaaaacgt tcccgccgat cgtctcgatc ttggcgttcc
     5881 cgttttcatc ggagctcgtt accaattcac agtgcattgg aaccagtccc gagtcgccga
     5941 cggagcggcg caattccagg tgtgccagca gctttgcatg gctatcgatg gcggccgcgc
     6001 taccgcgtgt gtagcagatc atgtctccct gcctcggcct ggtcgtggcc acgtcgcagg
     6061 ctcgataggc atattccgtg aactccccgc gaacttctgc cgaggatgtc aacacagcct
     6121 tgtcgacgta gtc
//
LOCUS       DASWIY010000196         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1981298, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000196 DASWIY010000000
VERSION     DASWIY010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981298"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1903)
                     /locus_tag="VGA59_07180"
     CDS             complement(260..1903)
                     /locus_tag="VGA59_07180"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX9719501.1"
                     /translation="MTTEGHTLAELSQLRQEVELLRAERQQFLEQLQKKNREIDALQH
                     RLQQLLRRYFGRSAEKLDPKQLQLFETLLDQLAPVVDPTPEPEPAARSNTNGHGRRRI
                     PKDLPRRTVIHDLAEDDKPCPCCGIMRHVIGRETSEQLDYIPAQLTVIEHVRLKYGCR
                     ACEQNASESGPQIVVADKPSTAIDKGLAAPGLLAHVMVSKYADHLPLHRQERILARYG
                     IEISRSTMCGWARQCAEMLRPLHERMIELLRLSKVIHTDDTPVKVQDRELDRTRTGRF
                     WVYLGDRSHPYTVFDYTPSRSRDGPRRFLERWSGYLQADAFGGYDGIYAGEVGGTVTE
                     VACWSHARRKFYDARESDSTASNQALAYIRLLYAVETEARQRANALRKQRDLCDDEAG
                     RSPGAPLCAELALDLRREKTLPRLAQFKAWLESQPASRGGHVLPKSPMGQAIAYALNQ
                     WEALCVYAADGDLAIDNNAAENALRCVAVGRNNWLFCGSDNGGATAAIHFSFMATCRR
                     HEIDPFAYLRDVLTRLPATPINEIDQFLPDRWAPEAPAN"
     gene            complement(1970..2347)
                     /gene="tnpB"
                     /locus_tag="VGA59_07185"
     CDS             complement(1970..2347)
                     /gene="tnpB"
                     /locus_tag="VGA59_07185"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX9719502.1"
                     /translation="MLKLPNLGELDSGLHVRVYLCTTPTDMRKGFDTLAALVRAQLKQ
                     DPLSGSLFLFVGRGKDRIKLLYWDTDGFAIWYKRLEEGTFRLPSPKQAGASVELKASE
                     LAMLLAGIDLTSVKRRKRFARSA"
     gene            complement(2341..2757)
                     /locus_tag="VGA59_07190"
     CDS             complement(2341..2757)
                     /locus_tag="VGA59_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9719503.1"
                     /translation="MATRDRSKEAFWRRMMRRQAGSGMSVRAWCGRHDLSEASFYWWR
                     RRLAPSGRPADKPGRHKAGTPQFVPVRIAADQADATSDGRPDCIEIILPGNQRVRVLG
                     AVNRRALADVLAVLRSGDIAAVRGTNHAVGAEAAAC"
BASE COUNT          523 a          938 c          929 g          552 t
ORIGIN      
        1 ccattcttgc tgaagaggat tgcatttctc ctggcgtcac cgttccatcc gtcgaaatct
       61 ctgtgacagc gagaaagccg caaaaccaca ggcgaagtaa aacaactcgc taatccaatc
      121 ctcgctgtca aagcggattg atgaccaccg gctgatacct gtgtggaaac ttcacgtcgt
      181 aaccagttca ttcgtaagcg tacggcgaac cacagggcgc gtcgcagtca cggcggctcg
      241 gcgtgaagtg gtggcgcggt cagttcgcgg gggcttccgg tgcccagcgg tcgggtagga
      301 attgatcgat ttcgttgatg ggcgtggcgg gcaggcgggt cagcacgtcg cgcaggtagg
      361 cgaacgggtc gatctcgtgc cgccggcagg tggccatgaa gctgaagtgg atggcggctg
      421 tggcgccgcc attgtccgag ccgcagaaga gccagttgtt ccgcccgaca gccacacagc
      481 gcagggcgtt ctcggctgca ttgttgtcga tggccaagtc gccgtccgca gcgtacacgc
      541 acagcgcctc ccactgattc agcgcgtacg caatcgcctg gcccattggg ctcttgggca
      601 gcacgtgtcc gccgcgcgac gccggctgcg actccagcca ggccttgaac tgcgccaggc
      661 gcggtagagt cttctctcga cgaaggtcca gggccaactc ggcgcagagt ggcgcgcccg
      721 gcgaccggcc ggcttcgtca tcgcagagat cgcgctgctt acgcagagcg ttggcgcgct
      781 gcctcgcttc agtctccacc gcatacagca gccggatgta ggccagcgcc tgattgctgg
      841 cggtggaatc cgattcgcgg gcgtcgtaga acttgcggcg cgcatgcgac cagcaggcca
      901 cctcggtgac cgtaccgccc acctcgcctg cgtagatgcc gtcgtacccg ccgaatgcat
      961 cggcctgtaa atacccgctc catctttcca ggaaccgacg cggtccgtcg cggctgcggc
     1021 tgggcgtgta gtcgaacacc gtataaggat gactgcgatc gcccaggtag acccagaacc
     1081 ggccggtccg cgtgcgatcg agttcgcgat cctgcacctt caccggcgtg tcgtcggtgt
     1141 gaatcacctt ggacaagcgc agcagttcga tcattcgttc atgcaagggt cgtaacatct
     1201 cggcgcactg ccgggcccaa ccgcacatgg tcgagcggga gatctcgatg ccgtaccgcg
     1261 ccaggatgcg ctcctgacga tgcagcggca gatgatcggc gtacttgctc accatcacgt
     1321 gggccagcag gcccggtgca gccaggccct tgtcgatcgc cgtcgagggc ttgtcggcca
     1381 ccacgatctg cggcccggac tccgacgcat tctgttcgca tgcccggcac ccatacttga
     1441 gccgcacgtg ctcgatgacg gtgagctggg ccggaatgta gtcgagttgt tcgctggtct
     1501 cccggccgat cacgtgacgc atgatcccgc agcagggaca gggcttgtcg tcttcagcca
     1561 ggtcgtggat caccgtccgc cgcggcaggt ccttggggat gcgccgccgc ccgtgaccgt
     1621 tggtgtttga cctggctgcg ggctccggct cgggtgtcgg atcaaccacc ggcgccagtt
     1681 gatcgagcag cgtttcgaag agttgcaatt gcttcggatc gagcttctct gcggaacggc
     1741 cgaagtagcg gcggagcagt tgctgcaatc gatgctgcag cgcgtcgatc tcgcggttct
     1801 tcttctgcag ttgctcgagg aattgctggc gctcggcgcg aagcagctcg acttcctgtc
     1861 gaagctgact cagttcggcg agcgtgtgac cctccgtggt catgcttcat tatacacgcg
     1921 catgcgcgcg gaagttcacc ggcgcgcaag aaaaaatctt tgcaggcgat cacgcgctgc
     1981 gcgcaaagcg cttgcggcgc ttgacgctgg tcagatcgat gcctgccagc agcatggcca
     2041 actcgctagc cttgagctct acactggcgc cggcctgctt cggactcggt agccggaacg
     2101 tgccttcctc gaggcgcttg taccaaatgg caaatccatc ggtatcccag tagagcagct
     2161 tgatgcggtc cttgcctcgc ccgacgaaga ggaagaggct gcccgaaagc gggtcttgct
     2221 tgagctgcgc gcgcaccagt gccgccagcg tgtcgaagcc tttgcgcatg tcggtcggcg
     2281 tggtgcacaa gtagacgcga acgtgcaagc cggaatcgag ttcgccgagg ttgggcagct
     2341 tcagcatgcc gccgcctccg cgccaacagc atggttcgtg ccacgtacag cagcgatgtc
     2401 gcctgaacgc aaaacggcca gcacatcagc cagcgcccgc cggttcaccg cgccaagcac
     2461 gcgtacgcgt tgattgccgg ggaggatgat ctcgatgcaa tcgggccggc catccgacgt
     2521 tgcatccgct tgatcggcgg cgattcgcac gggtacgaat tgcggcgtgc cagccttgtg
     2581 gcggccgggc ttgtccgccg gtctgccgct gggcgccagg cgacgacgcc accaatagaa
     2641 cgatgcttca gacaggtcat gccggccgca ccaggcccgc accgacatgc cgcttccggc
     2701 ctgacgtcgc atcatccgcc gccagaacgc ttccttcgac cgatcacgcg tcgccatgac
     2761 tgcctcctca aatcacggaa gcatcatccc tcagcaaggc agcggattga agatggggtt
     2821 cgccgagcgc ttacggggga aacggccagg caacgctcgt catttgaact gactgcgtcg
     2881 ccatgattcc ttccgagccg gcggatggca ggaactgatt actatgccca ccacctggcg
     2941 tg
//
LOCUS       DASWIY010000197         4414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3087048, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000197 DASWIY010000000
VERSION     DASWIY010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3087048"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..715)
                     /locus_tag="VGA59_07195"
     CDS             complement(62..715)
                     /locus_tag="VGA59_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719504.1"
                     /translation="MLRHALFALTASLGFLSPAGASSLDAPDAQLRWRLGFGGPAQSL
                     QTGYALTVGYRSGEPDSPAAQLLELDVSDRAAFARLAGLPLFGRDFTMQEAAGADPVA
                     AESLPWYARKWIWWTAGGLALTAAASGGSSGETTLCTGVCNQSDTGNSGTSINGVSDE
                     GIGCVDDTCAVPCESDNKPAGCVGLTALPAVSRYTFDPERIRWLDAGTGHMGDLIAR"
     gene            complement(750..1406)
                     /locus_tag="VGA59_07200"
     CDS             complement(750..1406)
                     /locus_tag="VGA59_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719505.1"
                     /translation="MVSIRTALALLVGLNTLSPVASAGVDGGLPEARLTWRLGFGDAG
                     QPLQTGYGLTVGYRGLDGAPAEQLLELDVSDQATLARLAGLPLFQRDYRARQNDGGTT
                     AEAGSRPWYARRWVLWTAGGLAASLAAVGGGGGDNTVCTGVCNQDGGPSGRTTGITYS
                     DGDVYAGCVEGQCVVCPDGSVASRCLVGMPSRAMVTTDPERDRWLDAGTGHMGDLIAR
                     "
     gene            complement(1519..1965)
                     /locus_tag="VGA59_07205"
     CDS             complement(1519..1965)
                     /locus_tag="VGA59_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719506.1"
                     /translation="MNVFCKSLLLLTGMAVVMPAAASDPGDAAEARLSWQVGFGGPGN
                     TFGKGYYSLTLAHRPADAGVPALKLAELAVSGAGTHARLMGVPLAPQDYQANMADTRI
                     EGLPSWMTLDVIWWVVSGVALTVIVLDKANDDDADPIQGTGGTGGT"
     gene            2149..2430
                     /gene="rpsP"
                     /locus_tag="VGA59_07210"
     CDS             2149..2430
                     /gene="rpsP"
                     /locus_tag="VGA59_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX9719507.1"
                     /translation="MVKIRLSRAGAKKRPFYHVVATDSRNPRDSKFLERLGYYNPKAV
                     GGEQKLVVDQARIEYWTQNGAQVSDRVQFLLSPAKAEAKAEAKAETAAA"
     gene            2439..2927
                     /gene="rimM"
                     /locus_tag="VGA59_07215"
     CDS             2439..2927
                     /gene="rimM"
                     /locus_tag="VGA59_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX9719508.1"
                     /translation="MSGQIEVGRVVGAFGIKGWIRVRSYSTPPEGLLRYKRWSISGRD
                     WEVLDGHAQGEFVVASLAGLADRSAAEAMRGGVIEVPRSALPKAKRGEFYWTDVLGSE
                     VVSTKGAKLGTLSSVGSNGAQDVMVVTGERERLIPAVAGAIVQSVDVKGKRIVVEWEP
                     EY"
     gene            2941..3690
                     /gene="trmD"
                     /locus_tag="VGA59_07220"
     CDS             2941..3690
                     /gene="trmD"
                     /locus_tag="VGA59_07220"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX9719509.1"
                     /translation="MKIEVLTLFPEFVEQVGRYGIVRRAVESGKLVLTTRDIRDTSEN
                     KWRRVDDRPYGGGPGMVMNAGPVAKAIGAAKAAVGEGAKVVALSPQGEPMTQQWVQRL
                     AAEPGLVVLCGRYEGIDERTQYFVDVELSLGDFVLSGGELAAMALVDAVARLIPGALG
                     DEQSAENDSFSAGLLDHPHYTRPEEWQGQRVPDVLLSGDHKAVRRWRLKRALGATWLK
                     RPDLLDNVQLDDEQDELLREFIAERQQKDGR"
     gene            3695..4042
                     /gene="rplS"
                     /locus_tag="VGA59_07225"
     CDS             3695..4042
                     /gene="rplS"
                     /locus_tag="VGA59_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX9719510.1"
                     /translation="MNIIEQIEAEQVAKKKIPAFKAGDTIEVKVKVKEGDRERLQAFE
                     GVVIAIKNRGLNSSFTVRKTSHGEGVERVFQTYSPQVAEIQVKRKGEVARAKLYYLRE
                     LAGKAARIKEKLS"
     gene            4197..>4414
                     /locus_tag="VGA59_07230"
     CDS             4197..>4414
                     /locus_tag="VGA59_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEX9719511.1"
                     /translation="MKAVFPRIQRLPPYVFNIVGDLKQVARARGEDIIDFGMGNPDQP
                     TPQHIVDKLVEAASRRGHRHTHRYSVSKG"
BASE COUNT          777 a         1386 c         1596 g          655 t
ORIGIN      
        1 cccacgcagt ggggagaggt cggcgcgaag cgccgggtga gggggttctt ttcgaagcgc
       61 ctcagcgcgc gatcaggtca cccatgtgcc cggtgccggc gtcgagccag cggatccgct
      121 ccggatcgaa ggtgtagcgg gagacggccg gcagcgccgt caggccgacg cagcccgccg
      181 gcttgttgtc cgactcgcag gggaccgcgc aggtgtcgtc gacacagccg ataccctcgt
      241 cgctgacgcc gttgatgctc gttccggagt tgcccgtatc gctctggttg cagacgccgg
      301 tgcagagggt ggtctcacca ctgctgcctc cgctcgccgc tgcggtcagg gccaggccac
      361 cggcggtcca ccagatccac ttgcgcgcgt accaaggcaa cgattccgcc gcgaccgggt
      421 cggcgcctgc cgcttcctgc atcgtgaagt cgcgcccgaa cagcggcagc ccggccaggc
      481 gcgcaaaggc ggcgcgatcc gagacgtcca gttcgagcag ttgcgcggcc ggcgaatcgg
      541 gttctccgga gcggtagccc acggtcaggg cgtagccggt ctgcagggat tgcgcggggc
      601 cgccgaagcc gaggcgccag cggagctgcg cgtcgggcgc gtcgaggctc gatgcgccgg
      661 ccggagacag gaaacccagg ctggcggtga gggcgaacag tgcatgtcga agcaagacgg
      721 actcctgcga ggaattggca aaagagaaat cagcgcgcga tcaggtcgcc catgtggccg
      781 gtgccggcat cgagccagcg gtcgcgctcg gggtccgtgg tgaccatggc gcgcgaaggc
      841 attcccacga gacagcgcga cgcgacgctg ccatcggggc agacgacgca ctggccttcg
      901 acgcagccgg cgtagacatc gccatcggag taggtgatgc cggtggtgcg gccactcggg
      961 ccgccgtcct gattgcagac gccggtgcaa acggtgttgt ccccgccgcc gccgcccacg
     1021 gccgccaggg aggcggcaag gccgcccgcg gtccatagca cccaccggcg cgcataccac
     1081 ggccgcgagc ccgcttccgc agtcgtgccc ccgtcgtttt gccgggcacg gtaatcgcgc
     1141 tggaacaggg gcaagccggc cagccgggcc agggtcgctt gatcggacac atcgagttcc
     1201 agcagctgct cggccggagc gccgtcgagg ccgcggtacc ccacggtcag gccgtaaccg
     1261 gtctgcagcg gctgcccggc atcaccgaag ccgaggcgcc aggtcaggcg ggcctccggc
     1321 aggcccccgt cgaccccggc agaggccaca ggtgacagtg tgttcaggcc gacaagcagg
     1381 gccagggcgg tgcggatgct caccatcgat tcactccagg aaagggttct gccgggacaa
     1441 cgctacggcc ggaactttat tcccgggagg tgggaccggc aatggtaaat cgggattgac
     1501 cgcccgaacc cgccaagctc aggttccgcc ggtgcccccg gtgccctgga tcgggtcggc
     1561 atcgtcgtcg ttggccttgt cgagcacgat caccgtgagc gccaccccgc tgacgaccca
     1621 ccagatcacg tccagcgtca tccagctggg caggccctcg atccgcgtgt cggccatgtt
     1681 cgcctggtaa tcctgcggcg ccagcggcac ccccatcagc cgagcgtggg tgccggcgcc
     1741 cgagacggcc agttcggcga gtttcagggc ggggacgccc gcgtccgccg gccggtgggc
     1801 cagggtcagg gagtaatacc ccttcccgaa cgtattgccg ggcccgccga agcccacctg
     1861 ccaggacaag cgtgcctcgg cggcgtcgcc gggatcggat gccgcagcgg gcatcacgac
     1921 ggccatgccc gtgagcagga ggaggctctt gcagaaaacg ttcatggtcg aaacccgttg
     1981 gaagcctgac cccactgtaa agtcgcccgc cgggtcggcg gcagtggtca agatctagca
     2041 gccgggcgct cggggtttgg cgcgatccaa gtctttcttt ttggggccga acggctacaa
     2101 tacgcgccct ttcgcggcct accggccgcg ttttcagttg atggatccat ggtcaaaatc
     2161 agattgtcac gggccggcgc gaaaaagcgt cccttttacc acgtcgtggc gacggacagc
     2221 cgtaacccgc gcgacagcaa gttcctcgag cgcctgggtt actacaaccc caaggccgtc
     2281 ggcggcgagc agaagctcgt ggtcgaccag gcccgcatcg aatactggac ccagaacggc
     2341 gcgcaggtct ccgaccgcgt gcagttcctg ctgtccccgg ccaaggccga agccaaggcc
     2401 gaagccaagg ctgagacagc cgccgcctga gcgtcccggt gtccggccag atcgaagtcg
     2461 gccgggtcgt cggagcgttc ggcatcaagg gctggatcag ggtccggtcg tactcgacgc
     2521 ctcccgaagg tctgctgcgt tacaagcgct ggagcatttc cggccgcgac tgggaagtgc
     2581 tggacggcca cgcgcagggc gagttcgtgg tggcgagcct cgcagggctc gcggatcgca
     2641 gcgccgcgga agcgatgcgg ggcggggtga tcgaagtgcc gcgcagcgcg ctgccgaagg
     2701 cgaagcgggg cgagttttac tggaccgacg tgctgggcag cgaagtggtc agcaccaagg
     2761 gtgcgaagct gggaacgctg agcagcgtgg gcagcaacgg cgcgcaggac gtgatggtgg
     2821 tgacgggcga gcgtgagcgg ctgatcccgg cggtcgccgg agcgatcgtg cagagcgtcg
     2881 acgtcaaggg caagcggatc gtggtggagt gggaaccgga atattagtgc gcgggacgcg
     2941 atgaagatcg aagtgttgac gctgttcccc gagttcgtgg aacaggtcgg gcggtacggc
     3001 atcgtgcggc gcgccgtgga gtccgggaag ctcgtgctga cgacgcgcga catccgggac
     3061 accagcgaga acaagtggcg ccgcgtggat gaccggccgt acggcggtgg ccccggcatg
     3121 gtgatgaacg cgggaccggt ggctaaggcg attggcgcgg cgaaggccgc ggtcggcgag
     3181 ggcgcgaagg tggttgcgct gtcgccgcag ggcgagccga tgacccagca gtgggtccag
     3241 cggctggcag cggagccggg gctggtcgtg ctgtgcggcc gctacgaagg catcgacgag
     3301 cggacgcagt attttgtgga cgtggagctg tcgctgggcg atttcgtatt gtccggcggc
     3361 gagctcgcgg cgatggcgct ggtggacgcg gtggcgcggc tgattccagg cgcgctgggc
     3421 gacgagcagt cggcggagaa cgattcgttc tcggcggggc tgctggacca cccgcactac
     3481 acgcggccgg aagagtggca gggccagcgg gtgccggacg tgctgttgtc cggcgaccac
     3541 aaggcggtac ggcgctggcg gctgaagcgg gcgctgggag ccacctggct caagcggccg
     3601 gatttgctgg acaatgtgca gctggacgac gaacaggatg aactgctgcg cgaattcatc
     3661 gcggagcggc aacagaagga cggaaggtga acagatgaac atcatcgagc agatcgaagc
     3721 ggaacaggtg gccaagaaga agatcccggc gttcaaggcc ggcgatacga tcgaggtcaa
     3781 ggtcaaggtc aaggaaggcg accgcgagcg cctgcaggcc ttcgagggcg tggtcatcgc
     3841 gatcaagaat cgcgggctca actcctcgtt caccgtgcgc aagacctcgc acggcgaggg
     3901 cgtggagcgc gtgttccaga cctacagccc gcaggtcgcc gagatccagg tcaagcgcaa
     3961 gggcgaagtc gcccgagcca agctgtatta cctgcgcgaa ctcgccggca aggctgcgcg
     4021 catcaaggag aagctgtcgt aggtcggcaa tccattgccg acgcggcggc acaggaatgc
     4081 cgccctacaa aagccggcgc aagccggctt ttttgttaga gcggaatttc agggttgatt
     4141 ctgttgcgat gcactaaata ggtccttaca gttacgcgct ccctgggagt cttgacgtga
     4201 aagccgtttt tccgcgcatc cagcgcctgc cgccctacgt gttcaacatc gtcggcgacc
     4261 tgaagcaggt cgcacgcgcc cgcggcgagg acatcatcga tttcggcatg ggcaacccgg
     4321 accagccgac gccgcagcac atcgtcgaca agctggtcga agccgcttcg cgccgcggcc
     4381 atcgccacac gcaccgctac tcggtgtcga aggg
//
LOCUS       DASWIY010000198         5281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_491393, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000198 DASWIY010000000
VERSION     DASWIY010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491393"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..140
                     /locus_tag="VGA59_07235"
     tRNA            56..140
                     /locus_tag="VGA59_07235"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:90..92,aa:Leu,seq:cag)
     gene            complement(448..1830)
                     /gene="rimO"
                     /locus_tag="VGA59_07240"
     CDS             complement(448..1830)
                     /gene="rimO"
                     /locus_tag="VGA59_07240"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX9719512.1"
                     /translation="MSEVDSPEAKTPKVGFVSLGCPKALTDSELILTQLSAEGYETSK
                     TFEGADLVIVNTCGFIDDAVRESLDTIGEALAENGRVIVTGCLGAKPGEGGGNLVRQV
                     HPSVLAVTGPHATREVMDAVHANLPKPHDPFTDLVPPAGIKLTPRHYAYLKISEGCNH
                     RCTFCIIPSMRGDLVSRPIGDVLKEARALFESGVKELLVISQDTSAYGVDLKYRTGFW
                     DGKPIRTRMLELVRELGEMAEPYGAWVRLHYVYPYPSVDEVIPLMATGKVLPYLDVPF
                     QHSHPDVLGRMKRPASGEKNLERIQRWREACPQIVIRSTFIAGFPGETEVEFDHLLQF
                     MREARIDRAGCFAYSPVEGAAANAIPGMLPQALREERRARFMAVAEEVSASKLRERVG
                     ATMQVLVDSAPALGRKGGVGRSYADAPEIDGTVRLLPPEKLSKRLAVGEFTQARVVGT
                     EGHDLVAVPF"
     gene            complement(1959..2504)
                     /gene="phaR"
                     /locus_tag="VGA59_07245"
     CDS             complement(1959..2504)
                     /gene="phaR"
                     /locus_tag="VGA59_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEX9719513.1"
                     /translation="MQRKKATAAGKPAQRVIKKYPNRRLYDTDTSTYITLAEVKHLVM
                     DSESFVVRDAKTNEDLTRSILLQIILEEEAGGAPMFSENALANLIRIYGHASQAFMGS
                     YLEKNVQAFTDIQAKLAEQSKTLTPEMWAQFMSLQNPLMQGMMGNYVEQSKNIFDKMQ
                     EQMAKQAEQMLGAFGISPGKR"
     gene            2783..3706
                     /locus_tag="VGA59_07250"
     CDS             2783..3706
                     /locus_tag="VGA59_07250"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX9719514.1"
                     /translation="MSILTSIGNTPLIELKRVAPPGSARIVAKLEWANPTGSMKDRMA
                     LAAIEGAEADGRLKPGGTVVEYTAGTTGISLAAVCAAKGYGLRVVFSDAFSEEKAVMM
                     KALGAQIVIVPSDNKRITESLIKRMIDESEVLSREPGHWWCDQLNNHDAVSGYLPMGE
                     EIWQQSGGRVDAFVQAVGTAHSIQGVARSLRRHRSSIRVVAVEPAESAVLSGQPSGSH
                     SIEGIGIGFVPPLWSPHEVDEIMAVSSEEAKAMARRLSREEGVFGGTSSGANVVAALR
                     IAERLGAGATVATVIVDSGLRYLSTDLYRSA"
     gene            complement(3783..3859)
                     /locus_tag="VGA59_07255"
     tRNA            complement(3783..3859)
                     /locus_tag="VGA59_07255"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3823..3825),aa:Val,seq:gac)
     gene            complement(3962..>5281)
                     /locus_tag="VGA59_07260"
     CDS             complement(3962..>5281)
                     /locus_tag="VGA59_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX9719515.1"
                     /translation="ALSWVHEQFTPIVEEFDDYIAKPKANGYQSLHTIVRDKAGRPIE
                     IQIRTQAMHDHAEHGVAAHWAYKEAGSKGYSGVSASGEYDAKIAVLRQLLAWERDLAG
                     SGAGLFEDRIYVLTPDAAIVELPQGATPVDFAYSVHTNLGHRCRGAKIDGAMVPLNTP
                     LQNGQTVEVITVKEGGPSRDWLNADLGFLASHRAKAKVRAWFNERIKHETVARGREAV
                     EKLLQREGKTATKLEDLAGQLGFKSAEDLFAVVGKDEYSLRNIETLLRPAEAPLPQDE
                     FLLLKKSRQAEKTPKGGVLVVGIDSLMTQLAKCCKPAPPDAIRGFVTRGKGVSIHRSD
                     CSNFRELAARSAERVIEVEWGKSKSDGAAVYPVDVGVEAADRQGLLRDISEVFAKEKM
                     NVIGVQTQSLKGTAWMTFTVEIADSARLNKVLGLVAEVQGVRWAKRR"
BASE COUNT          873 a         1720 c         1689 g          999 t
ORIGIN      
        1 cgctgtgctg atgtgtccaa tatttttgat ataaaatcct tttctttcct gaaatgccca
       61 ggtggcggaa ttggtagacg cactagtttc aggtactagc gagtaacatc gtgggggttc
      121 gagtcccttc ctgggcacca atcattgatc ataaagccgg ccttgccggc tttgttattt
      181 gtgcccgggg catctcagtt ctccatggcg ctgcgcgcca cccgcagcgt atgaaatcgt
      241 gcggtcagtg cgcgggccac cgcggaaccg gctttgccgg gccgctggtg ggccttgcag
      301 gccgcacggc cgcgagacgc taccattcct cgacctgtcc aaaccgacgc aggtcggtct
      361 ggagccgcag gtctcggccc cctcgagggg gagagggcga agcccgaggg ggcatcgccg
      421 aaggcgcttc gggggtgggc tttcacttca gaacggcacc gcaaccaggt cgtgcccctc
      481 ggttcccacc acgcgcgcct gggtgaactc gcccaccgcc agccgtttgc tgagtttttc
      541 cggcggcagc agcctcacgg tcccgtcgat ctccggcgcg tcggcgtagg agcggcccac
      601 gccgcccttg cggccgagtg ccggggccga atcgaccagc acctgcatgg tcgccccgac
      661 ccgctcgcgc agcttgctcg ccgacacttc ctccgccacg gccatgaagc gggcgcgccg
      721 ctcttcacgc aatgcctgcg gcagcatgcc cgggatggca ttggcggccg cgccttcgac
      781 cgggctgtag gcaaagcagc cggcgcgatc gatcctcgct tcacgcatga actgcagcag
      841 gtgatcgaac tccacctcgg tttcccccgg gaatccggcg atgaacgtgc tgcggatcac
      901 gatctgcggg cacgcttcgc gccagcgctg gatgcgctcc aggtttttct cgccgctggc
      961 cggccgcttc atgcggccca acacgtcggg gtggctgtgc tggaacggca cgtccaggta
     1021 aggcaggacc ttgccggtgg ccatcagcgg aatcacttca tccacggacg ggtacggata
     1081 cacataatgc aatcgcaccc aggcgccgta cggttccgcc atttcgccca attcccgcac
     1141 cagttccagc atgcgcgtcc tgatcggctt gccgtcccag aagccggtcc ggtatttcag
     1201 gtccacccca taggccgagg tgtcctggct gatcaccagc agttccttca cgccgctctc
     1261 gaacagggcc cgggcctcct tcagcacgtc gccgatgggc ctgctcacca ggtcgccgcg
     1321 catcgacggg atgatgcaga aggtgcagcg gtgattgcag ccttcactga tcttcaggta
     1381 ggcgtagtgc ctgggcgtga gtttgatccc cgccggcggc accaggtcgg tgaatggatc
     1441 gtgcggcttg ggcagattcg catgcacggc gtccatgact tcgcgcgtgg cgtgcggtcc
     1501 ggtgacggcc agcaccgacg ggtggacctg gcgcaccagg ttgccgccgc cctcgcccgg
     1561 cttggcgccc aggcagccgg tgacgatcac gcggccgttt tcggccagcg cttcgccgat
     1621 cgtgtccagg ctttccctga cggcgtcgtc gatgaagccg caggtattga cgatcacgag
     1681 gtcggccccc tcgaaggtct tcgaggtctc gtacccctcg gcgctcagct gggtaaggat
     1741 gagttcagaa tccgtcaggg ccttggggca gcccaggctg acgaatccga ccttcggtgt
     1801 cttggcttca ggggagtcaa cttcgctcat gccccaaatt gtcccagatg atgtcattgc
     1861 gggctcgacc cgcaatccat gaccctgaat tcatggatcc cggatcgggc ctgccccgga
     1921 ctcgatccgg gatgacaact ccggacgatc tgcaagcttc agcgttttcc aggtgaaatc
     1981 ccgaacgccc cgagcatctg ctcggcctgc ttggccatct gctcctgcat cttgtcgaag
     2041 atgttcttgg actgctcgac gtaattgccc atcatgccct gcatcagcgg attctgcagg
     2101 ctcatgaact gcgcccacat ctcgggcgtc agggtcttgg attgctcggc cagcttggcc
     2161 tggatgtcgg tgaaggcctg cacgttcttt tcgaggtagc tgcccatgaa ggcctgggac
     2221 gcatggccgt agatgcggat caggttggcc agcgcgttct cgctgaacat cggcgcaccg
     2281 ccggcttctt cttccaggat gatctgcagc aggatgctgc gtgtgaggtc ctcgttggtc
     2341 ttggcgtcgc gcaccacgaa ggactcgctg tccatcacca ggtgcttgac ttcggccagc
     2401 gtgatgtagg tggaggtgtc ggtgtcgtaa agccggcggt tgggatattt cttgatgacg
     2461 cgctgcgcgg gtttgccggc ggcggtggct ttcttgcgtt gcacgggctg actcctggct
     2521 cgggcgctgc tgtcggcgct cgatgtattg cactgcaaca cattctaggg agccagccga
     2581 accgtaaggc ccaaggttta cccctagggt gagtctccgc ttgattgacg tcatgggaag
     2641 cccaggcagt tccagcccaa cgcccaacgc tgcggcgttc atggccgcag ctctgccgtg
     2701 agtgaaagga atgcgtgatg ggtgacaagg gccgcaaaca ggtgcagagt cttggcgcgc
     2761 aggatcgcaa gcggagacag cgatgagcat tctcaccagc atcggaaaca cccctttgat
     2821 cgagttgaag cgcgtggcac cgcccggctc ggcgcgcatc gtggcgaagc tcgagtgggc
     2881 caatcccacc ggaagcatga aggaccggat ggcgcttgcc gccatcgaag gtgccgaggc
     2941 ggacggccgc ctcaaacccg gcgggaccgt ggtggagtac acggccggca ccaccggcat
     3001 atcgcttgcc gccgtatgcg ccgcgaaggg ctatgggctg cgggtcgtgt tctcggatgc
     3061 cttcagcgaa gaaaaagccg tgatgatgaa agcactgggc gcacagatag tgatcgttcc
     3121 cagtgacaac aagcgcatta ccgagtcgct gatcaagcgc atgatcgacg agtcggaggt
     3181 cctcagccgg gaaccggggc actggtggtg cgaccagttg aacaaccacg acgccgtgag
     3241 cggctatctc cccatgggcg aagagatatg gcagcaaagc ggcggccgcg tcgacgcgtt
     3301 cgtgcaggcc gtgggcaccg cccactcgat ccagggcgtg gcacggtcgc tgcgacggca
     3361 ccgcagcagc attcgcgtcg tggcagtgga accggccgaa tccgccgtcc tttccgggca
     3421 gccgtcggga tcgcacagca tcgaaggcat cggcatcggc ttcgtgccgc cgctatggag
     3481 cccgcacgag gtggacgaaa tcatggcggt ctccagcgaa gaggccaagg ccatggcgcg
     3541 gcggctgtcg cgtgaggaag gcgtgttcgg cggaacctcg tcgggcgcga acgtcgtcgc
     3601 cgcactgcga atagccgaac gcctgggcgc cggtgccacg gttgccaccg tgatcgtcga
     3661 ttcggggctg cgctacctga gcacggacct gtaccggagt gcctgagtca agacaggcat
     3721 gggtgggaag tgaaaaagcc ccgccgatcg ttgccgtggc aagactcggg aggctcagga
     3781 attggtaggc gcgattggac tcgaaccaac gacccccacc atgtcaaggt ggtgctctaa
     3841 ccagctgagc tacgcgcctg aattcgtgtt cgagggagcg attgtagcag ggccggaagt
     3901 gggacatgga ttgcggatcg agtccgcaat gacaagaaac ccccccgcaa tgacagcccg
     3961 ttcaccgcct cttcgcccag cgcacgccct gcacctcggc caccaggccc agcaccttgt
     4021 tcaggcgggc cgagtcggcg atctccacgg tgaacgtcat ccaggcggtt cccttcagag
     4081 actgcgtctg tacgccgatc acgttcatct tctccttggc gaagacttcg gagatgtcgc
     4141 gcagcagtcc ctgccggtcg gccgcttcca cgccgacatc gaccgggtac accgccgcgc
     4201 cgtccgattt ggacttcccc cactcgacct cgatcacgcg ctcggcggag cgcgccgcga
     4261 gttcgcgaaa gttgctgcag tcgctgcggt ggatgctcac gcccttgccg cgcgtcacga
     4321 aaccgcggat cgcatcgggc ggggccggct tgcagcactt ggccagctgg gtcatcagcg
     4381 aatcgatgcc caccaccagc acgccgccct tgggcgtctt ctcggcctgg cgcgatttct
     4441 tcagcagcag gaactcgtcc tgcggcagcg gcgcctccgc cggccgcagc agggtctcga
     4501 tgttgcgcag ggaatattcg tccttgccca ccacggcgaa caggtcctcg gccgacttga
     4561 aaccgagctg gcccgccagg tcttcaagct tcgtcgccgt cttgccctcg cgctgcagca
     4621 gcttctccac tgcctcgcga ccccgcgcca ccgtctcgtg cttgatccgt tcgttgaacc
     4681 aggcgcgcac cttggccttg gcccggtggc tggccaggaa ccccaggtcg gcgttgagcc
     4741 agtcgcgcga cggcccgccc tccttcacgg tgatgacttc cacggtctgg ccgttttgca
     4801 gcggtgtgtt cagcggcacc atcgccccat cgatcttggc gccgcggcag cggtggccga
     4861 ggttcgtgtg cacgctgtag gcgaagtcca ccggcgtcgc tccctgcggc agctccacga
     4921 tggcggcatc cggcgtcagg acatagatgc ggtcctcgaa cagtcccgcg cccgagccgg
     4981 cgaggtcccg ctcccaggcc agcagctgcc gcaataccgc gatcttggcg tcgtactctc
     5041 cgctggccga cacgccggag tagcccttgc tgcccgcctc cttgtaggcc cagtgcgcgg
     5101 cgacgccatg ttccgcgtgg tcgtgcatgg cctgggtgcg gatctggatt tcgatcggcc
     5161 gccccgcctt gtcgcgcacg atcgtatgca gcgactggta gccattggcc ttgggcttgg
     5221 cgatgtagtc gtcgaactcc tcgacgatgg gcgtgaactg ctcgtgaacc catgacagcg
     5281 c
//
LOCUS       DASWIY010000199         6021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_109373, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000199 DASWIY010000000
VERSION     DASWIY010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109373"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..1808)
                     /gene="narH"
                     /locus_tag="VGA59_07265"
     CDS             complement(285..1808)
                     /gene="narH"
                     /locus_tag="VGA59_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEX9719516.1"
                     /translation="MRIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRPGMEYAWFNNV
                     ETKPGIGYPKEWENQDRWNGGWVRHADGSIHPRQGARWKLLMKIFANPNLPEIDDYYE
                     PFTYDYAHLQSAPEMKAAPTARPRSLIDGRQMDKIVWGPNWEEILGGEFAKRSRDRNF
                     DDIQKDMYAEYENTFMMYLPRLCEHCLNPSCVASCPSGSIYKREEDGIVLIDQDKCRG
                     WRMCVSGCPYKKIYYNWKSGKAEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDA
                     DRIQQAASVEKDRDLYQAQLDIFLDPYDPKVVAQAHLDGVPDNWLEAARASPVYKMAV
                     QWKVALPLHPEYRTLPMVWYVPPLSPISAAVNAGQLQANGQIPDVSQLRIPVKYLANM
                     LTAGDTGPVIRALERMLAMRSYQRGKHVHKRIDTAVLHQVGISQADVEEMYRIMAIAN
                     YEDRFVIPTAHREYAENAFNVRGGCGFSFGNGCSDGVSNASLFGVEKKRTIPIKAEV"
     gene            complement(1899..5705)
                     /locus_tag="VGA59_07270"
     CDS             complement(1899..5705)
                     /locus_tag="VGA59_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEX9719517.1"
                     /translation="MSHFLDRLTYFTHPRESFAGGHGHVTSEDRTWEDAYRNRWASDK
                     IVRSTHGVNCTGSCSWKIYVKGGIVTWEIQQIDYPRTRWDMPNHEPRGCARGASYSWY
                     LYSANRLKYPMVRGRLLKAWRQARQSMPPVQAWAAIVGNEATRRDYQQVRGLGGFARS
                     TWDEVNEIVAAANIHTIKHHGPDRIIGFSPIPAMSMVSYAAGTRYLSLIGGVCMSFYD
                     WYCDLPPSSPQVWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGTT
                     TVAITPDYSEVAKLCDIWMHPKQGTDAAVAMAMGHVILKEFYFPEGGARSAYFDEYVR
                     RYTDMPMLVTLKERTLANGEIVQVPDRYVRASDFPVGLGQDNNPEWKTVALDEDGDVV
                     VPQGAIGFRWGPDGRSDKGQWNLEDKEANKGRTVRLKLSLLEGRESDGDTARVGFPYF
                     GGIRSEHFPANDQAGDVLVRTVAVTHVALRDGKALVATVFDLLVANYGVARGLAGEHA
                     ALSFDDDIPYTPAWQEQITGVPRDQVIAVARAFAQNAHDTQGRSMVIIGAAMNHWYHS
                     DMNYRGVINMLMMCGCIGQSGGGWAHYVGQEKLRPQTGWTPLAFALDWVRPPRQMNST
                     SFFYAHTDQWRYEKLGVEEILSPLADRAAFGGSMIDYNVRAERMGWLPSAPQLQANPL
                     QVVRDAQAAGADPKEYAAKALKNGSLKLSCGDPDHPDNWPRNMFVWRSNILGSSGKGH
                     EYFLKHLLGTSNGVQGKDLGAEDAKPTDVVWHDKAPEGKLDLLVTLDFRMSTTCLYSD
                     IVLPTATWYEKNDLNTSDMHPFIHPLGAAVDPAWQSKSDWDIYRGFAQKFSELCAGHL
                     GVERDLVLTPLGHDTPAELAQPFGVADWARGECDLVPGKTAPQMTVVERDYPNTYKRF
                     TALGPLMSTLGNGGKGIGWNTQDEVTQLGQLNGVVAEAGASQGMPGIVTDIDACEVIL
                     QLAPETNGHVAVKAWEALSKQTGRDHAHLARHREDEKIRFRDIQAQPRKIISSPTWSG
                     LESEKVSYNAGYTNVHELIPWRTLTGRQHFYLDHPWMLAFGEGLSSYRPPVDLKTTAA
                     MAGAKPNGNPEIALNFITPHQKWGIHSTYTDNLIMLTLSRGGPCVWISEEDARKAGIE
                     DNDWIELFNVNGALTARAVVSQRVKPGMVMMYHAQEKIVNTPGSEISGVRGGIHNSVT
                     RVVLKPTHMIGGYAQLSYGFNYYGTIGTNRDEFVLVRKMNKVDWLDTPVAEQLIRPTL
                     DQGETA"
     gene            complement(5846..>6021)
                     /locus_tag="VGA59_07275"
     CDS             complement(5846..>6021)
                     /locus_tag="VGA59_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775787.1"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEX9719518.1"
                     /translation="TAAMAAYGGFFIPKSYGTSITMTGGPEAALYTFIVFYALCIAVT
                     WWYYSRKNAPMPC"
BASE COUNT          916 a         1933 c         2017 g         1155 t
ORIGIN      
        1 cccaatgccg ggcctgcact atgcacgtgc cgaggcatga cgctgcggcc cggccccgca
       61 atccatgccg gtggagggcg ctgacgccgc atggatcccg ggtcaagccc gggatgacag
      121 cagcatgtgg gacaggtttc acttcagttc tccgacgcat gaagtcgcgc ggccggtgcg
      181 ggggcaaccg cggaaccggc ttcgccgggc cgctggtggc gcccccttga gggggagagg
      241 gcgaagcccg agggggcatc gccgaaggcg cttcgggggt gggctcatac ttctgccttg
      301 atagggatcg tgcgtttttt ctccacgccg aacaggctgg cgttggagac cccgtccgag
      361 cagccgttgc cgaagctgaa gccgcagccg ccgcgcacgt tgaacgcgtt ctcggcgtat
      421 tcgcggtgcg ccgtcggaat gacgaaacgg tcctcgtaat tggcgatggc catgatgcgg
      481 tacatctcct ccacgtcggc ctggctgatg ccgacctggt gcagcaccgc ggtgtcgatc
      541 cgcttgtgaa cgtgcttgcc gcgctggtag gagcgcatgg ccagcatgcg ctcgagcgcg
      601 cgtatcaccg gcccggtgtc tcctgccgtc agcatgttgg cgaggtactt caccggaata
      661 cgcagctggc tcacatccgg tatctggcca ttggcctgga gctgcccggc gttgacggcc
      721 gcgctgatgg gcgacagcgg cggcacgtac cagaccatcg gcagcgtgcg gtactcggga
      781 tgcagcggca gcgccacctt ccattgcacg gccatcttgt acaccgggct ggcgcgcgcc
      841 gcttccagcc agttgtccgg cacgccgtcg aggtgcgcct gggcgacgac cttggggtca
      901 tacgggtcca ggaagatgtc cagctgtgcc tgatacaggt cgcggtcctt ttccacactg
      961 gccgcctgct ggatgcggtc agcgtcgtac aacagcacgc ccaggtagcg gatgcggccc
     1021 acgcaggttt cggagcagac ggtgggctgg cccgcttcga tgcgcgggta gcagaagatg
     1081 cacttctcgg ccttgccgct cttccagttg taatagatct tcttgtaagg gcagcccgac
     1141 acgcacatgc gccagccgcg gcacttgtcc tggtcgatca gcacgatgcc gtcttcctcg
     1201 cgcttgtaga tcgagccgga ggggcacgaa gccacgcacg aggggttcag gcagtgctcg
     1261 cacaggcggg gcaggtacat catgaaggtg ttctcgtact ccgcgtacat gtccttctgg
     1321 atgtcgtcga agttccggtc gcggctgcgc ttggcgaact cgccccccaa aatctcctcc
     1381 cagttggggc cccagacgat cttgtccatc tggcgcccgt cgatcaggct gcgcgggcga
     1441 gccgtgggcg cggccttcat ctcgggcgcg gattgcaggt gcgcgtagtc gtaggtgaag
     1501 ggctcgtagt agtcgtcgat ctcgggcagg ttggggttgg cgaagatctt catcaacagc
     1561 ttccaccgcg cgccctggcg cggatggatg gatccgtcgg cgtggcgcac ccagccgccg
     1621 ttccaccggt cctggttctc ccactccttc gggtagccga tccccggctt ggtttcgacg
     1681 ttgttgaacc aggcgtattc catgccgggg cggctggtcc agacgttctt gcaggtgacc
     1741 gaacaggtgt ggcagccgat gcacttgtcc agattgagca ccataccgat ctgcgcgcgg
     1801 attctcatgc ggcctcctgg gtcatgctgc ctcccgccga gccgcctcaa gggaggcaag
     1861 ccccctcagg gggcagcgaa cgaatgtgag cgtgggggtc atgctgtttc tccctggtcg
     1921 agcgtgggcc ggatgagctg ctcggcaacg ggggtgtcga gccagtccac cttgttcatc
     1981 ttgcgcacca gcacgaactc gtcgcggttg gtgccgatgg tgccgtagta gttgaagccg
     2041 taggacagct gcgcgtagcc gccgatcatg tgcgtgggct tgagcaccac gcgcgtgacg
     2101 gagttgtgga tgccgccgcg caccccgctg atctccgagc ccggcgtgtt cacgattttt
     2161 tcctgcgcgt ggtacatcat caccatgccg ggcttgacgc gctggctcac caccgcgcgg
     2221 gccgtcagcg cgccgttgac gttgaacagc tcgatccagt cgttgtcctc gatgcccgcc
     2281 ttgcgtgcat cctcctcgct gatccacaca cacgggccac cgcggctgag cgtgagcatg
     2341 atgaggttgt cggtgtaagt ggagtggatg ccccacttct ggtgcggcgt gatgaagttc
     2401 agtgcgatct ccgggttgcc gttgggcttg gccccggcca tcgccgccgt tgtcttcagg
     2461 tccaccggcg ggcggtagct gctcaggccc tcgccgaagg ccagcatcca ggggtgatcc
     2521 agatagaagt gctgccgccc ggtgagcgtg cgccacggaa tcagctcgtg cacattggtg
     2581 tagcccgcgt tgtaggaaac cttttcgctc tccagcccgc tccaggtcgg cgaggaaata
     2641 atcttgcgcg gctgcgcctg aatgtcccgg aagcggatct tctcgtcctc ccgatgccgt
     2701 gccagatgag cgtggtcgcg cccggtctgt ttcgagagcg cctcccaggc ttttaccgcc
     2761 acgtggccgt tggtttccgg ggcgagttgc aggatgactt cgcaggcgtc gatgtccgtc
     2821 acgatgccgg gcattccctg gctggccccg gcttcggcca cgacgccatt gagctgcccc
     2881 agttgcgtga cctcgtcctg cgtgttccag ccgatgccct tgccgccgtt gcccagcgtg
     2941 gacatcagcg gacccagtgc ggtgaagcgc ttgtaggtgt tgggatagtc gcgctcgacc
     3001 accgtcatct gcggcgcggt cttgcccgga accagatcgc actcgccgcg cgcccaatcg
     3061 gcgacgccga agggctgtgc cagctccgcg ggtgtgtcgt gccccagcgg ggtcaacacc
     3121 agatcgcgct ccacgcccag atggcccgcg cacagctcgc tgaacttttg agcgaagccc
     3181 cgatagatat cccagtcgct cttcgactgc cacgccgggt ccaccgccgc gcccagcggg
     3241 tggatgaacg gatgcatgtc gctggtgttg aggtcgttct tctcgtacca ggtggccgtg
     3301 ggcagcacga tgtccgagta caggcaggtc gtgctcatgc ggaagtccag ggtgaccagc
     3361 aggtccagct tgccttccgg ggccttgtcg tgccacacaa cgtcggtcgg cttggcgtcc
     3421 tcggcaccga ggtccttgcc ctgcaccccg ttgctggtgc ccagcaggtg cttgaggaaa
     3481 tactcgtggc ccttgcccga agaacccagg atgttggagc gccacacgaa catgttgcgc
     3541 ggccagttgt cggggtgatc ggggtcgccg cagctcagtt tgagcgaacc gttcttcagc
     3601 gccttggccg cgtattcctt cgggtccgcg cccgccgctt gcgcgtcgcg caccacctgg
     3661 agcggattgg cttgaagctg cggtgccgaa ggcagccagc ccatgcgttc ggcccgcacg
     3721 ttgtaatcga tcatgctgcc gccgaaggcg gcgcggtcgg ccagcgggga caggatttcc
     3781 tccacgccca gcttctcgta gcgccactgg tcggtgtggg cgtagaaaaa ggaggtggag
     3841 ttcatctgac gcggcgggcg aacccagtcc agcgcgaagg ccagcggggt ccagccggtc
     3901 tgcgggcgca gcttttcctg ccccacgtaa tgcgcccagc cgccgccgct ttgcccgatg
     3961 cagccgcaca tcatcagcat gttgatgacg ccgcggtagt tcatgtcgct gtggtaccag
     4021 tggttcatgg cggcgccgat gatgaccatg gaccgccctt gcgtgtcgtg cgcgttctgg
     4081 gcgaaagccc gcgccacggc gatcacctgg tcgcgcggca cgccggtgat ctgttcctgc
     4141 caggcggggg tgtagggtat gtcgtcatcg aacgagagcg ccgcgtgctc accggccagg
     4201 cccctggcga caccgtagtt ggccaccagc aggtcgaaca ccgtcgccac cagcgccttg
     4261 ccatcgcgca gcgcgacatg cgtgacggca acggtgcgca cgagcacgtc gccggcctgg
     4321 tcgttggcgg ggaaatgctc gctgcggatg ccgccgaaat acgggaagcc aacgcgcgcc
     4381 gtgtctccgt cgctctcgcg cccctccagc agcgacagtt tcagccgcac ggtgcgcccc
     4441 ttgttcgcct ccttgtcctc caggttccac tggcccttgt cggaccggcc gtcgggcccc
     4501 cagcggaagc cgatggcacc ctggggaacc accacgtcgc cgtcttcgtc cagcgcgacg
     4561 gtcttccatt ccgggttgtt gtcctggccc aggccgaccg ggaagtccga cgcgcgcaca
     4621 tagcggtccg gcacttgcac gatctcgccg ttggccagcg tgcgttcctt cagcgtcacc
     4681 agcataggca tgtcggtgta gcgacgcaca tattcgtcga agtaggcact gcgcgcgcca
     4741 ccctccggaa agtaaaactc cttgaggatg acatggccca tcgccatggc cacggccgca
     4801 tcggtgccct gcttgggatg catccagatg tcgcacagct tggcaacctc ggagtagtcg
     4861 ggcgtgatcg cgaccgtggt ggtgcccttg tagcgcacct cggtgaagaa gtgcgcgtcg
     4921 ggcgtgcgcg tctgcggcac gttggagccc caggcgatga tgtagctgga gttgtaccag
     4981 tcggccgatt cgggcacgtc ggtctgctcg ccccacacct gcgggctcga tggcggcagg
     5041 tcgcaatacc agtcgtagaa gctcatgcag acgccgccga tgagcgagag gtagcgcgtg
     5101 cccgccgcat agctgaccat cgacatcgcc gggatcggcg aaaagccgat gatgcgatcc
     5161 gggccatggt gcttgatggt gtggatgttg gccgccgcga cgatctcgtt cacctcgtcc
     5221 caggtcgaac gcgcgaaacc gccgaggccg cgcacctgct ggtagtcgcg ccgcgtggct
     5281 tcattgccca cgatcgccgc ccacgcctgg accggcggca tgctctggcg ggcctggcgc
     5341 caggccttga gcaggcgccc gcgcaccatc gggtacttca ggcggttggc gctgtacagg
     5401 taccagctgt agctggcgcc gcgggcgcag ccgcgcggct cgtggttggg catgtcccag
     5461 cgggtgcgcg ggtaatcgat ctgctggatt tcccaggtga cgatgccgcc cttgacatag
     5521 atcttccagg agcacgatcc ggtgcagttc acgccgtggg tgctgcgcac gatcttgtcg
     5581 gaggcccagc ggttgcggta ggcatcttcc caggtgcggt cctcgcttgt cacgtggccg
     5641 tggccaccgg cgaagctttc gcgggggtgc gtgaaatagg tcagccggtc gaggaagtgg
     5701 ctcattcgtt gttctccggg aatttgtcat cccgggcttg acccgggatc catgcggcct
     5761 tccaccatgg atgcacccgg atccggtccg gggcaggctc cgtcaagccc ggcatgacag
     5821 gcacttggat ggcgggcact gttggtcaac atggcatcgg tgcgttcttg cggctgtaat
     5881 accaccaggt gacggcgatg cacagcgcgt agaagacgat gaaggtgtac aacgccgcct
     5941 ccgggccgcc ggtcatggtg atggaggtgc cgtagctctt ggggatgaag aagccgccgt
     6001 aggccgccat ggcggcggtg a
//
LOCUS       DASWIY010000200         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1218389, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000200 DASWIY010000000
VERSION     DASWIY010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218389"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VGA59_07280"
     CDS             complement(<1..527)
                     /locus_tag="VGA59_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719519.1"
                     /translation="MNTSPTVPEVKVPRILVVDDMPDNLFLMNGLFEDRYVVIQAPSG
                     KDALNVVMSANPPDMVLLDIMMPDMDGYEVLRRIRQHPPTANIPVIFLTALATRQDER
                     LGMDLGAVDYLTKPVDPELVVQRVEAHVRATAHARRIEALSEKLARQLSPNDWERLFH
                     GSDLETIHFEEKVQTV"
     gene            complement(673..822)
                     /locus_tag="VGA59_07285"
     CDS             complement(673..822)
                     /locus_tag="VGA59_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX9719520.1"
                     /translation="MLYTIALVLLILWLLGLVTSVTAGGLIHILLVIAIVVVLLRVIS
                     GRKVL"
     gene            complement(832..1296)
                     /locus_tag="VGA59_07290"
     CDS             complement(832..1296)
                     /locus_tag="VGA59_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719521.1"
                     /translation="MTHVPVAYPADIGTPTGIVGAIRSLHPAALPFFIAIVVFSTVAM
                     ANVPVARWLESSLEYARQLPTPEAVGEAAQAALPADDGAVQARVRTRCDACGIVEAIR
                     RIDPVGALPATYEFTVRLRDGSTRLSSDASPSKWRAGDAIMLIGGASSATQH"
     gene            complement(1323..1763)
                     /locus_tag="VGA59_07295"
     CDS             complement(1323..1763)
                     /locus_tag="VGA59_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719522.1"
                     /translation="MKTSSRPAPAVLTPPNGHQAIRLYALTLAATVALLVAGTAAAAG
                     ANLSQAQQRYQQERAVCLSGQSNQDRATCLKEAGAALYEARRGGLDTGDDRDLGHNRM
                     MRCKALPAQDREDCARRMHGEGVTTGSAQQGGVLRELARPVAPN"
     gene            complement(1833..2045)
                     /locus_tag="VGA59_07300"
     CDS             complement(1833..2045)
                     /locus_tag="VGA59_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX9719523.1"
                     /translation="MKAAFAAGVLAVAAVVGLSGCAGMSTRDKNVVGGAAIGGVAGAV
                     LTGGSAIGTVGGAAVGGLIGNEVGKR"
     gene            complement(2042..>2213)
                     /locus_tag="VGA59_07305"
     CDS             complement(2042..>2213)
                     /locus_tag="VGA59_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX9719524.1"
                     /translation="IFKEPALKSAEINVETFKGRVQLSGFVSSRDNINRAVTVAQGVK
                     GVTSVGNDMRLK"
BASE COUNT          350 a          720 c          739 g          404 t
ORIGIN      
        1 accgtctgca ctttttcctc gaagtggatg gtctcgaggt cggagccgtg gaacaggcgt
       61 tcccagtcgt tgggggacag ctgccgcgcg agcttctcgg acagcgcctc gatccggcgc
      121 gcgtgggccg tggcgcgcac atgcgcttcg acccgttgca ccaccagctc gggatcgacc
      181 ggcttggtga gatagtccac ggcacccagg tccatgccca ggcgctcgtc ctgacgcgtc
      241 gccagcgcgg tgaggaagat gacggggatg ttggcggtgg gcggatgctg gcggatgcgc
      301 cgcagcactt cgtagccgtc catgtccggc atcatgatgt ccagcagcac catgtccggc
      361 gggttggccg acatcaccac attgagcgca tctttgcccg agggcgcctg gatcaccacg
      421 tagcggtcct cgaacagccc gttcatcagg aacaggttgt ccggcatgtc gtcgaccacc
      481 aggatgcgtg gcactttgac ttccgggacg gtcgggctgg tgttcatggc gattcccctt
      541 ggctgtaact gggtgttgcc atgaaaccat gccgggccgg ttcagccgta ggcgaaacag
      601 cctgccggct tagggaaatt gcctagccgg cgcgagccga tgcaaaccgc cgataactcg
      661 gcgtcgcctc gctcagagta ctttgcgccc gctgataacg cgcaacagca caacgacgat
      721 cgcgatcacc agcaggatat ggatcagacc tcccgcggtg acggatgtga ccagtcctag
      781 caaccacagg atgagcagga caagcgcgat ggtatagagc acgatgttct ttcagtgttg
      841 ggtggcggag ctggccccgc ctatgagcat gatggcgtcg ccggcacgcc atttcgacgg
      901 gcttgcatcg ctgctcaggc gggtcgatcc gtcgcgcaga cgcacggtga attcataggt
      961 cgcgggcaac gcccccacag gatcgatgcg ccggatggct tcgacgatcc cgcaggcgtc
     1021 gcaacgtgtc ctcacgcgcg cctggacagc cccgtcatcc gccggcagcg cggcctgcgc
     1081 cgcctcgccc accgcctcag gcgtgggcag ctgccgcgca tattccagcg agctttccag
     1141 ccaccgggcg accggcacgt tcgccatggc caccgtgctg aacacaacga ttgcgataaa
     1201 gaagggcaag gctgccgggt gcagcgaacg gatcgccccg actattcccg tgggggtgcc
     1261 gatgtctgcc gggtaagcga ctgggacgtg agtcatttgg ggccttgctt gttcgaggtt
     1321 ggctagttgg gagcgaccgg cctcgccagt tcgcgcagga cgccgccctg ctgggcgctt
     1381 ccggtggtga cgccttctcc gtgcatccgc cgcgcgcaat cttctcggtc ctgcgccggc
     1441 agtgccttgc aacgcatcat gcggttgtgc ccgaggtcgc ggtcgtcacc ggtgtcgagg
     1501 ccgccgcgcc tggcttcata caaggcggcg ccggcctcct tcaggcaggt cgcacggtcc
     1561 tgattggact ggccgctcag gcagaccgcg cgctcctgtt gatagcgctg ttgggcctgc
     1621 gacaggtttg cgcccgccgc ggccgctgtg ccggcaacga gcagggcgac cgtcgcggcc
     1681 agggtcaagg cgtacaaccg gatggcctgg tggccattgg gcggcgtaag gacggcgggg
     1741 gctggacgcg aagaggtttt catggggatt tccttgggtg ttgttcaggc gccgacgccg
     1801 gccctttccg gggccgcacg ccgcccgcgg ggctatcgct tgccgacctc gttgccgatc
     1861 aggccgccaa ccgcggcacc gccgacggtt ccgatggcac tgccacccgt gaggaccgcg
     1921 ccggcgacgc cgccgatggc cgcgcccccg acgacgttct tgtcacgtgt ggacattccg
     1981 gcgcagccgg acagacccac gaccgctgca acggccagta ctcccgcagc gaacgcggct
     2041 ttcacttgag cctcatgtcg ttgccgacgg atgtgacgcc cttgacgcct tgcgccacgg
     2101 tgacggcccg gttgatattg tcccggctgc tgacgaatcc gctcagttgg acacggccct
     2161 tgaaggtctc gacgttgatt tcggcggatt tgagcgccgg ttccttgaaa atg
//
LOCUS       DASWIY010000201         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3427457, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000201 DASWIY010000000
VERSION     DASWIY010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3427457"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /gene="gatB"
                     /locus_tag="VGA59_07310"
     CDS             <1..874
                     /gene="gatB"
                     /locus_tag="VGA59_07310"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899691.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEX9719525.1"
                     /translation="GSFRCDANVSVRKPGDPLGTRREIKNLNSFKFMQQAIDYEIRWQ
                     IEEIEDGRTIQQATVLFDPDTGETRAMRTKEDAADYRYFPDPDLPPLVIAPEWVERVR
                     SEMAELPRVMAARFVKDYGLPEYDATTLTQSKEMADFFVAVAETSGQPKLASNWIMGE
                     VSRRMNSEIVSMAAVKNRFGSTRLAKLIQRIADGTISNNAARQVFDALWSGEGQEVDA
                     IIEAKGLKQMNDSGALEKIIDEVIAASPDNVAQFKAGKDKAFNALVGQAMKASKGKAN
                     PAQVNELLRRKLGA"
     gene            complement(988..1653)
                     /locus_tag="VGA59_07315"
     CDS             complement(988..1653)
                     /locus_tag="VGA59_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX9719526.1"
                     /translation="MRGLTERVIAGGLFLGGLFGQAGAEGIYTCIDAKGHRHTADRPI
                     AACIDREQKVLNASGTVKRRIGPSLTAQELAAEEEKARKAAEERARLAEEKKRNRALL
                     TRYPNRAAHDKERLAALALAEDAIAAARKSTDELVALRKRLETELEFYQNDFSKVPPI
                     LKRQVDENEHHIQAQQRFVANQNSEKKRINAQFDEELARLRQLWASSAAPATAAVPAA
                     AKR"
     gene            complement(1664..2344)
                     /gene="pyrE"
                     /locus_tag="VGA59_07320"
     CDS             complement(1664..2344)
                     /gene="pyrE"
                     /locus_tag="VGA59_07320"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX9719527.1"
                     /translation="MVIVGGQDRLAQDFVQFSVESGVLRFGRFKTKAGRLSPYFFNAG
                     LFDDGAKLGRLAQFYAKRILGSGIEFDMLFGPAYKGIPLAAAVAIELARLGRNVPFAY
                     NRKEPKDHGEGGSLVGAPVKGRVMIVDDVMSAGTAARESIAVIQAAGATPHAVAIALD
                     RQEKAIENGVDVNHSAVQYVRDQLGLKVCSIARLEDLLHYLQRHSGDDIGAYHEQVLA
                     YRQRYGVD"
     gene            2355..3149
                     /locus_tag="VGA59_07325"
     CDS             2355..3149
                     /locus_tag="VGA59_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX9719528.1"
                     /translation="MFKLTSLNLNGIRSAASKGVQAWLAKTKPDCICVQEVKAQAGDV
                     LGRFEEMAGLRGHFHFAEKKGYSGVAVYTRHAPSDVIVGYGSPEFDVEGRYVELRFDT
                     PRRKLSVISCYFPSGSSGEERQQAKFRFLDEFDPHLARLKTQREFILCGDVNIAHKEQ
                     DLKNWRGNRKNSGFLPEERAWMTKLLDETGLVDVYRHLQPETTGECYTWWSNRGQAWA
                     RNVGWRLDYHLATSELARGARFAEIHKDQRFSDHAPLTIDYDIEVG"
     gene            complement(3181..>3514)
                     /locus_tag="VGA59_07330"
     CDS             complement(3181..>3514)
                     /locus_tag="VGA59_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9719529.1"
                     /translation="LLPFKLGDDDNPTGRADGPMSEGILQRLQEERRLMYVGITRAQR
                     SLAVSWTRKRKKGREMIAAQPSRFIAEMALEKGTTQEDPREKLRALRAEFAKKALATA
                     AAQDAAAA"
BASE COUNT          629 a         1151 c         1115 g          619 t
ORIGIN      
        1 aggctccttc cgctgcgacg ccaacgtctc ggtgcgcaag cccggcgatc cgctgggcac
       61 gcgccgcgaa atcaagaacc tgaacagctt caagttcatg cagcaggcga tcgactacga
      121 gatccgctgg cagatcgagg agatcgagga cggccgcacg atccagcagg ccaccgtgct
      181 gttcgacccg gacaccggcg agacccgcgc catgcgcacc aaggaagatg ccgccgatta
      241 ccgctacttc cccgatccgg acttgccgcc gctggtgatc gcgcccgaat gggtcgaacg
      301 cgtgcgctcc gaaatggcgg agctgccgcg cgtgatggcc gcgcgcttcg tcaaggacta
      361 cggcctgccc gagtacgacg ccacgacgtt gacgcagagc aaggaaatgg cggacttctt
      421 cgtggccgtg gcggaaactt ccggacagcc gaagctcgcg agcaactgga tcatggggga
      481 agtgtcgcgt cggatgaaca gcgagatcgt ctccatggcg gccgtcaaga atcgcttcgg
      541 ttcaacccgg ctagccaaac tcattcaacg catcgctgac ggcacgatct ccaacaacgc
      601 cgcgaggcag gtgttcgatg cgctgtggag cggcgagggc caggaggtcg acgcgatcat
      661 cgaggccaag ggcctcaagc agatgaacga cagcggcgcc ctggaaaaaa tcatcgacga
      721 ggtgatcgcc gccagccccg acaacgtggc ccagttcaag gccggcaagg acaaggcgtt
      781 caacgcgctg gtgggccagg ccatgaaagc gagcaagggc aaggccaacc cggcccaggt
      841 caacgagctg ctgcgccgca agctgggcgc ctgacgagag gggctaacac ttcgcggccg
      901 ccggcaaggc tgtgtcatcc cgggcttgac ccgggatcca tgaattcaga agcatggatt
      961 gcgggtcaag cccgcaatga caacacctta gcgcttcgcc gccgccggca ccgccgcagt
     1021 ggcgggcgcg gcgctgctgg cccacagctg cctgagcctg gctagttcct cgtcgaactg
     1081 ggcgttgatc cgcttctttt cgctgttctg gttcgcgacg aaccgctgct gtgcctggat
     1141 gtggtgctcg ttctcatcca cctggcgttt gagtattggc ggaaccttgg aaaagtcgtt
     1201 ctggtagaac tccagttcgg tctccaggcg cttgcgcaat gccacgagct cgtccgtgct
     1261 ttttctcgcg gcggcgatcg cgtcttccgc cagcgcgagc gcggccaggc gctccttgtc
     1321 gtgggcggcg cggttgggat aacgcgtgag cagcgcccga ttacgcttct tctcctccgc
     1381 gagccgggcg cgttcctccg cggccttgcg ggctttttcc tcttctgccg cgagctcctg
     1441 ggccgtgagg ctggggccga tcctgcgctt cacggtgccg ctcgcgttga gcaccttctg
     1501 ctcgcggtcg atgcaagccg cgatcgggcg gtcggccgtg tggcggtgcc ccttggcatc
     1561 gatgcaggtg tagatgccct cagcgccggc ttgcccgaac aagccgccca agaacaagcc
     1621 cccggcaatg acgcgttccg tcaaaccccg cacgtttgtg tccttaatcc acgccgtagc
     1681 gctgacggta agcgagtacc tgctcatggt acgcgccaat gtcatcgcca ctgtgccttt
     1741 gcagatagtg aagcaaatcc tcgagccgcg caatcgagca aaccttcagc cccagctggt
     1801 cgcgcacgta ttgcacggcg ctgtggttca cgtccacgcc gttctcgatg gccttctctt
     1861 gccggtccag cgcgatcgcc accgcgtgcg gcgtggcccc ggccgcctgg atgacggcaa
     1921 tggattcgcg ggccgcggtt cccgccgaca tgacgtcgtc cacgatcatc acccggccct
     1981 tgacgggcgc gcccaccagc gagccgcctt caccgtggtc cttgggctcc ttgcggttgt
     2041 acgcaaacgg cacattgcgg cccaggcgcg ccagctcgat ggccaccgcg gcggccaggg
     2101 ggataccctt gtacgccggc ccgaacagca tgtcgaactc gatgcccgac cccaggatgc
     2161 gctttgcata gaattgcgcc agccggccca gcttggcgcc gtcgtcgaac aggcccgcgt
     2221 tgaagaaata aggcgacaag cgtccggcct tggtcttgaa gcggccaaag cgcagcacgc
     2281 cggattcaac cgagaactgc acgaaatcct gagccagccg atcctgcccg ccaactatca
     2341 ccatggggac ttccttgttc aaactgacca gccttaacct gaatggcatc cgttcggctg
     2401 ccagcaaagg ggttcaggcc tggcttgcaa aaaccaagcc ggattgtatt tgcgttcagg
     2461 aggtcaaggc ccaggccggg gatgtcctgg gccgattcga ggaaatggcg ggactcaggg
     2521 gccacttcca cttcgccgaa aagaaagggt attccggcgt ggccgtttac acccggcacg
     2581 cccccagtga cgtgatcgtc ggctatggat cgcccgaatt cgatgtcgag ggccgctacg
     2641 tggagctgcg attcgacacg ccgcgccgca agctgtccgt catcagctgc tatttcccca
     2701 gcggatcctc gggcgaggag cgccagcagg ccaagttccg tttcctggat gaattcgacc
     2761 cgcacctggc gcggcttaag acgcagcgcg aattcatcct gtgcggcgac gtcaacatcg
     2821 cccacaagga gcaggacctg aagaactggc gcggcaaccg caagaacagc ggcttcctgc
     2881 ccgaggagag ggcctggatg acaaagttgt tggacgaaac gggcctggtc gacgtttacc
     2941 gccatctcca gccggagact accggggagt gttacacgtg gtggagcaat cgcggccagg
     3001 cttgggccag gaacgtcggc tggcggctgg actaccacct ggcgacttcg gagctggcca
     3061 ggggcgccag gttcgccgag atccacaaag accagcgttt cagcgaccat gcgcccctaa
     3121 cgatcgacta cgacatcgag gtgggataac ctctgatcca agccatcgcg gcacgcggtg
     3181 tcacgcggcg gccgcgtcct gcgccgccgc ggtggccagc gccttcttcg cgaattcggc
     3241 gcgcaaggcc ctgagctttt cgcgcgggtc ttcctgggtc gtgccttttt ccagcgccat
     3301 ctcggcgatg aagcggctgg gctgggccgc gatcatctct cggcccttct tgcgcttgcg
     3361 cgtccagctg acggccagcg agcgctgggc ccgggtgatg cccacgtaca tcaggcggcg
     3421 ctcttcctgc aggcgctgca ggatgccttc ggacatcggc ccatcggcgc gccctgtggg
     3481 gttgtcgtcg tcgccgagct tgaacggcag cagc
//
LOCUS       DASWIY010000202         6991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_852631, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000202 DASWIY010000000
VERSION     DASWIY010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852631"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1172)
                     /locus_tag="VGA59_07335"
     CDS             complement(81..1172)
                     /locus_tag="VGA59_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9719530.1"
                     /translation="MLRRNVETQPYGRCSVCSLQLKQCHAWQSNGYSFALVVLALSLV
                     LVPAGWPQKLLAAVLLGVVVWQGLVNHKRTDELIHGQHRLMSLTQELRGKQLLIEQQN
                     DNLAAQVDARTAELRDANMRLAAANLELLELDKLRSAVLSNVSHELRTPLTGILGSAQ
                     NLRDGLAGGLTQAQQEYVQMIESDSGRLIRVVNELLEWGRLQSGHIQLQRSPVALYPL
                     LDEVFMLMRPAAQQKAVKLELADGDPLVSVEADADKLKQILINLLDNAVKFSRPDSSV
                     RVRAEPMTTALRILVEDQGPGVAPEDVPHLFERFFRGRAAGVTPGSGLGLAIAKNLAR
                     LHGGDITLKSSPGQGSVFTLNLPASGAAA"
     gene            complement(1165..1680)
                     /locus_tag="VGA59_07340"
     CDS             complement(1165..1680)
                     /locus_tag="VGA59_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HEX9719531.1"
                     /translation="MNTDRELQYGPGMRVQRLTEAAFLVAALFTQDVRFAYVTFALTL
                     LQVLSPRWVPIARAVAWLVRFRGEHRIGDLYFDLDGIRGACALSVVVQAAALGLVWAG
                     YPMVGFLILTIPTASFLMAPTLGFCAGCWFYVLGRDWLARHGWLKRGFDDCSDITFDG
                     ESPRHQPELRA"
     gene            1889..2257
                     /locus_tag="VGA59_07345"
     CDS             1889..2257
                     /locus_tag="VGA59_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX9719532.1"
                     /translation="MAEESRELVVVITQGIDHELSSVGLTIANGGITAGLKVAIFLTS
                     AGVDIVRKKASDTTHVKPLDPLADMVRDFVARGGALYACTPCVKSRGYEQADFVEGVT
                     IAGSSVIHERIKRGAATLSF"
     gene            2274..3044
                     /locus_tag="VGA59_07350"
     CDS             2274..3044
                     /locus_tag="VGA59_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9719533.1"
                     /translation="MLPQVNRADLEGKVKAMYRDVAENPHGEFHFEMGRALAERLGYR
                     PADLDRIPAEAIDSFAGVGYYFHLLGDMESARVLDLGSGSGMDTFIASLIAGPTGTVI
                     GLDMTDSQRAKAEALRQRDGFGNVTYVKGYIDAAPFEDGSFDVIISNGVINLAVDKPQ
                     VFREIARLLRPGGQLAIADIVTDVLLPENISCNTTLWAACIGGAWQVERYKDAIESAG
                     LRVTAEQANEQYRFLSDNAQGATKKFGVKSISLRADKA"
     gene            complement(3058..3477)
                     /locus_tag="VGA59_07355"
     CDS             complement(3058..3477)
                     /locus_tag="VGA59_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719534.1"
                     /translation="MKEQEASRSSQRGAAPVAARPPESADKFDSNTTAMFQAKMKRAP
                     VWFCSFLAGNSREKLRLRDELALMKGAWPLLMKQRNGGKWTPEEKVQLKAMIRSASSV
                     SPYLFIWALPGSVVLLPFLAWHLDRQRKSRIRKQSRL"
     gene            complement(3578..4039)
                     /locus_tag="VGA59_07360"
     CDS             complement(3578..4039)
                     /locus_tag="VGA59_07360"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9719535.1"
                     /translation="MHPYPHHYRAQAEGSATGSVRVAGKSLPALETYPPPEFDGPSGY
                     WSPETLLVASIADCYILSFRAVARASQLEWESLSVEVEGVLDRVAGATRFVEFKLSPR
                     LSICSRDREPLARTVLEKSKRACLITNSMSGECELLPEISVCECASESEAA"
     gene            4208..6247
                     /locus_tag="VGA59_07365"
     CDS             4208..6247
                     /locus_tag="VGA59_07365"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEX9719536.1"
                     /translation="MAPPHIPQIRLYQNWLREKRGLSFDSYDALWRWSTTDLSAFWQS
                     VWDYFDLRSPTPYRAVLAEAKMPGAKWFEGAQFNYAQQVFRHVDKAHAAGFQAVIGRN
                     EKGEQRELSWPELRRQVASLALHLRQQGVQPGDRVAAYLPNIPEAMVAFLAVISVGGI
                     WSICAPDMGTNAVLDRFRQIEPKVLIACDGVSYGGRDFDRTAVVAELRAALPSVSHVI
                     VHRNLGVGDTAMKTLAPMTDMAHAMARDDALTAGFEPLWLPFDHPLWIVYSSGTTGLP
                     KPIVHGHGGTVLVALALKVLHNDIGCSYEENSWGERYHWYSSTGWVMWNAQVSGLLHG
                     TTCCIFDGNPGGKKSAGPSGSQDNPDWTVLWRFAADLGVTFFGAGAAFFANCMKANID
                     LSSCGNLRRVRALGTTGSPLPEEAQRWGTAQFEKIGTQDIWWCNISGGTDFAGAFIGG
                     NRELPQVPGQMQCRLLGCAVEAWDEQGRPVTGEVGELVCTQPIPSMPLFFWGDEGNQR
                     YLSSYFDMYPNVWRHGDWLKITPSGGCIIYGRSDATINRHGLRMGTSELYSAVEALTE
                     VLDAMVVDLEYLGRESYMPLFVVLRPGVALDEAVKGRINEAIRTALSPRFVPNEVFQV
                     AEIPRTLSGKKQELPIKKLLLGQPLEKVVNKDAMANPGCLDWYVAFANERLSSGA"
     gene            complement(6369..>6991)
                     /locus_tag="VGA59_07370"
     CDS             complement(6369..>6991)
                     /locus_tag="VGA59_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719537.1"
                     /translation="QMDLRAAAPGRDLVPGGCLGEEHRGLEVDLQDFVENFFRHLEQR
                     PPGPGPRWAGQDIHEVRTGERERLAIHYHQSYQVRSFQRNKPNFMLAHAASASPLVHL
                     FDEPQFNTQDGYIGFMLDFFRKLRRGGKLVFLCLHPNERYHIDILRESCERFIFVQKG
                     VVTQASDFDALMRDERVRGYLGALAPDVSLAAYSNTQRMDPGSSPG"
BASE COUNT         1297 a         2248 c         2263 g         1183 t
ORIGIN      
        1 ctcgggagcg gcccagcggt gtattcggaa aacctccgcg ctacgcgctg cggtattcgc
       61 ctcgggagcg gcccagcggc tcatgcggcc gcacccgaag cgggcaggtt caaagtgaaa
      121 acgctgccct ggcccggcga cgacttcagc gtgatatcgc cgccatgcag gcgcgccagg
      181 ttcttggcga tggccagacc caggccgctg ccgggtgtga cgcccgcggc gcggcctcgg
      241 aaaaaccgct cgaacaggtg cggcacatcc tcgggggcca cacctggccc ttgatcctcg
      301 accaggatgc gcagcgccgt cgtcatcggc tccgcgcgca cccgcacgct ggaatcggga
      361 cggctgaact tgacggcgtt gtccagcagg ttgatcagga tctgcttgag cttgtcggca
      421 tcggcctcga cgctgaccag ggggtcgcca tcggcgagtt cgagcttgac ggccttttgc
      481 tgcgccgccg gtcgcatcag catgaacact tcgtccagca gcgggtaaag cgccacaggc
      541 gaccgctgca gctggatgtg cccggattga agacggcccc actccagcag ttcattgacc
      601 acgcggatga gccggccgct gtcgctctcg atcatttgca catattcctg ctgggcctgt
      661 gtcagcccgc ccgccagacc gtcccgcagg ttctgcgcgg agcccaggat gcccgtcagc
      721 ggcgtgcgaa gctcgtgcga aacattggac agcacggccg accgcaactt gtcgagttcc
      781 agcagctcga gattggccgc cgccagccgc atgttggcat cacgaagctc ggcggtgcgt
      841 gcgtccacct gcgcggccaa gttgtcgttc tgctgttcga tcagcagttg cttgccccgc
      901 aactcctgcg tgaggctcat caggcggtgt tgaccgtgga tcagctcatc ggtgcgcttg
      961 tggttgacga ggccctgcca cacgaccacg cccagcagca cggccgccag cagcttttgc
     1021 ggccagccgg ccgggaccag caccagcgag agggccagga ccaccagcgc gaagctatag
     1081 ccgttggatt gccaggcatg gcactgtttg agctggagcg aacagacgct gcagcggccg
     1141 tagggttgcg tctcgacgtt gcgcctaagc acgcagttct ggttgatgcc tggggctttc
     1201 gccgtcgaag gtgatgtcgc tgcagtcatc gaagcctctc ttcagccagc catggcgggc
     1261 cagccagtcg cgccccagca catagaacca gcagccggcg caaaaaccca gcgtcggcgc
     1321 catgaggaac gatgccgtcg gaatcgtcag gatcaggaaa ccgaccatcg ggtagcccgc
     1381 ccacaccaga ccgagcgcgg cggcctgtac gaccacggac agcgcgcaag caccgcgaat
     1441 tccgtcgaga tcgaagtaca ggtcgccaat ccggtgctcg ccacggaaac gcacaagcca
     1501 tgccaccgcg cgcgcgatcg gcacccagcg cggcgacaac acctgaagaa gggtcaacgc
     1561 gaaggtgaca taggcaaagc gcacgtcctg ggtgaacagc gccgccacca ggaaagccgc
     1621 ctcggtcaag cgctgcacgc gcatgcccgg tccgtactgc agttcgcggt cggtgttcat
     1681 tggaaatcct cgatcaaagt ctagcgatta gggccgtgcg gcgccagcgg gttagccata
     1741 tccgaaagga catggcccat acgatttcgc gcatgggtgt ggcagccgtt gcaaccgcga
     1801 gttgcctgat tgcgcaggga aaaatgccgg agttggagct ggcacggcat tcgcaatgcc
     1861 caacggaccc aaccacaagg agatcgatat ggctgaagag agcagagagc tggtcgtcgt
     1921 catcacccaa ggcatcgacc acgagttgtc atcggtgggt ctcaccatcg cgaacggcgg
     1981 catcactgcc gggctgaaag tggccatctt cctcaccagc gccggcgttg acatcgtgcg
     2041 caagaaggcg tccgacacca cgcacgtgaa gccgctggat ccgctggccg acatggtgcg
     2101 cgatttcgtg gcgcgcggcg gcgcgctcta cgcctgcacg ccttgcgtca agtcacgcgg
     2161 ctacgagcag gcggatttcg tcgagggcgt gaccattgcc ggctcgagcg tgatccacga
     2221 gcggatcaag cggggcgccg ccaccctcag cttctgaccg aaggaggact cccatgttgc
     2281 cccaagtcaa ccgcgccgac ctcgaaggca aggtcaaggc gatgtaccgc gacgtcgcgg
     2341 aaaatccgca cggcgagttc catttcgaaa tgggacgggc gctcgctgag cggctcggct
     2401 accggccggc cgacctcgac cgtatcccgg ccgaagcgat cgattccttt gcaggggtcg
     2461 gctactactt ccacctgctg ggcgacatgg agagcgctcg tgtgctcgac cttggcagcg
     2521 gttcgggcat ggacacgttc atcgccagcc tgatcgcggg gcccaccggc acggtgatcg
     2581 gcctggacat gaccgattcg caacgcgcca aggcggaggc cctgcggcaa cgcgacggct
     2641 ttggcaacgt cacctacgtc aagggctaca tcgacgccgc gccgttcgag gatggcagct
     2701 tcgacgtgat catcagcaac ggcgtgatca atctggcggt cgacaagccg caggtctttc
     2761 gcgaaatcgc gcggctgctg cgccccggcg ggcagctggc gatcgcggat atcgtcaccg
     2821 acgtgttgct gccggagaac atcagctgca atacgacgct ctgggccgcc tgtatcggcg
     2881 gggcctggca ggtggagcgc tacaaggacg ccatcgagtc ggccgggctg cgcgtcacgg
     2941 cggagcaggc caacgagcag taccggttcc tgtcggacaa cgctcaaggc gcaacgaaga
     3001 aattcggcgt caagagtatc tcgctcaggg ccgacaaggc ctgaccgcct gccggcatca
     3061 aaggcgtgac tgcttgcgga tccggctctt gcgctgccgg tcgaggtgcc aggccaggaa
     3121 aggcagcaac accacggacc ccggcagcgc ccagatgaaa agataggggc tgaccgagga
     3181 agcggaccgg atcatggcct tgagctggac cttttcctcc ggggtccact tgccgccgtt
     3241 gcgctgcttc atgagcaggg gccaggcccc cttcatcagc gcaagctcgt cgcgcagcct
     3301 gagcttttcg cggctgttgc cggcgaggaa cgagcagaac cagaccggcg cgcgtttcat
     3361 cttcgcctgg aacatcgcgg tcgtattgct gtcgaacttg tcggcgcttt cgggtggacg
     3421 cgccgcaacc ggcgcggcgc cgcgttggct ggacctgctg gcctcttgct ctttcatggc
     3481 gcattctagg accggcgtaa gcgcgcggca aacccgtctt gacgaaaccg ccaaggtcag
     3541 gcagttgctc aaagcagcca ggtcagggtg gcattggcta cgctgcctcg gactcgctgg
     3601 cacattcgca cacgctgatc tcaggcagca gctcgcactc gccactcatc gagttcgtga
     3661 tcaggcaagc gcgcttggac ttttcgagca ccgtccgggc caggggctcc cggtcgcgcg
     3721 agcaaatgga cagccggggc gacagcttga attcgacgaa acgcgtggcc cccgcgacgc
     3781 gatccagaac cccttcgacc tcaacggaga gactctccca ctcgagctgg gaagccctgg
     3841 cgaccgcgcg aaaggaaagg atgtagcagt ccgcgatgga tgccaccagc agggtttctg
     3901 gcgaccagta gccgctgggc ccgtcgaact ccggcggcgg gtacgtttcc agcgcgggaa
     3961 gcgatttccc agcgacacgg acgctgcccg tggcgctgcc ctccgcttgc gcgcggtagt
     4021 ggtgtggata gggatgcatg gcgagcctgt gtatagtaga taagccacag cttatacaaa
     4081 ctggccccag ccccgcttga cataagccaa gtatctgcaa gtttcccctc ccggctaatg
     4141 gacgatccct gcgtgggccg acgcgaaatg atgagctggg ccgacccggc tttggtaatc
     4201 tcgccgcatg gccccgcccc acattcccca gatccgcctc taccagaact ggttgcgcga
     4261 aaagcgcggc ctttccttcg acagctacga cgcgctgtgg cgctggtcga ccacggatct
     4321 ttccgccttc tggcaaagcg tctgggacta cttcgacctg cgttcaccca cgccgtatcg
     4381 ggccgtgctg gccgaagcga agatgccggg ggcgaagtgg ttcgaaggcg cgcagttcaa
     4441 ttacgcgcag caggtgttcc gccatgtcga caaagcccac gccgccggtt ttcaggccgt
     4501 catcggccgc aacgaaaagg gcgagcagcg cgagttgagc tggcccgaac tgcggcgcca
     4561 ggtcgcttca ctggcattgc acctgaggca acagggcgtt cagccgggcg accgagtcgc
     4621 ggcctacctg cccaacatcc ccgaagcgat ggtcgccttt ctcgcggtga tcagcgtggg
     4681 tggcatctgg agcatctgcg cccccgacat gggcaccaac gccgtgctgg accgcttcag
     4741 gcagatcgag cccaaggtgc tgatcgcctg cgacggcgtg agctacggcg gccgcgactt
     4801 cgaccgcacc gccgtggtcg ccgagttgcg cgccgccctg cccagcgtca gccacgtcat
     4861 cgtgcaccgc aacctcggtg tcggcgacac tgcgatgaag acgctcgcgc ccatgacgga
     4921 catggcacac gcgatggcgc gcgacgatgc gctgaccgcg ggcttcgagc cgctgtggct
     4981 gcccttcgat catccgttgt ggatcgtcta ctccagcggc accaccggcc tgcccaagcc
     5041 catcgtgcac ggccacggcg gcaccgtgct ggtggccctc gcgctcaagg tgctgcacaa
     5101 cgacatcggc tgcagctacg aagaaaacag ctggggcgag cgttatcact ggtacagctc
     5161 caccggctgg gtgatgtgga acgcccaggt cagcgggctg ctgcatggca cgacctgctg
     5221 catctttgac ggcaatccag gtggcaagaa atcggcaggc ccctccggta gccaggacaa
     5281 tcccgactgg accgtcttat ggcgtttcgc ggccgatctg ggagtaactt tcttcggcgc
     5341 cggcgcggcg ttcttcgcca actgcatgaa agcgaatatc gatctttctt cctgcggcaa
     5401 cctgcggcgc gtgcgggcgc tgggcaccac cggttcgccg ctgccggaag aagcgcaacg
     5461 ctggggcacc gcgcagttcg agaaaatcgg cacgcaagat atctggtggt gcaacatctc
     5521 cggagggacc gacttcgccg gcgccttcat cgggggcaat cgcgagctgc cgcaggtccc
     5581 cggccagatg cagtgccgcc tgctcggctg tgcggtcgag gcctgggacg agcagggccg
     5641 gccggtaacg ggcgaagtgg gcgagttggt gtgcacccag ccgattccct cgatgccgtt
     5701 gttcttctgg ggcgacgaag gcaaccagcg ttacctgtcc tcgtacttcg acatgtatcc
     5761 caatgtctgg aggcacggcg actggctcaa gatcacgcca tctggcgggt gcatcatcta
     5821 tggccgcagc gatgcaacca tcaaccgcca tgggctgcgc atgggcacca gcgagttgta
     5881 cagcgcggtg gaggcgctga ccgaggtgct ggacgcgatg gtggtcgacc tggagtacct
     5941 cgggcgcgaa agctacatgc cgctgttcgt cgtcctgcgc ccgggcgtag ctctggacga
     6001 ggccgtgaag ggcaggatca acgaggcgat caggaccgcg ctgtcgccgc gcttcgtgcc
     6061 caacgaggtc ttccaggtcg ccgagatccc gcgcacgctg tcgggaaaga aacaggagtt
     6121 gccgatcaag aagctgctgc tgggccagcc gctcgaaaag gtcgtcaaca aggacgccat
     6181 ggccaatccg gggtgcctgg actggtatgt ggcgtttgcg aatgaacggc tgtcatctgg
     6241 ggcctgacca gggagtgact gtcatgccgg acttgaggag cctgccccgg actccgatcc
     6301 gggggcatcc acgacttcat ggattgcgga tcaggtccgc aatgacaaac cacgggcgtt
     6361 gaaagctgtc atcccggact tgatccggga tccatgcgtt gggtgttact gtaagccgcc
     6421 aatgacacat cgggggcgag agcacccagg tatccccgca cccgctcatc ccgcatcaaa
     6481 gcatcgaagt ccgatgcctg cgtgaccacg cccttctgca cgaagatgaa ccgctcgcaa
     6541 ctctcgcgca ggatgtcgat gtggtagcgc tcgttgggat gcaggcacag gaacaccagc
     6601 ttgccaccgc gccgcagctt gcggaaaaaa tcgagcatga agccgatgta gccatcctgc
     6661 gtgttgaact ggggctcgtc gaacaggtgc accagcggtg acgcgctggc ggcatgcgcc
     6721 agcatgaagt tgggcttgtt gcgctggaac gaacgtacct ggtaggactg gtggtagtga
     6781 atcgccagcc gctcgcgctc gccggtgcgc acttcgtgga tgtcctggcc tgcccagcgc
     6841 gggccgggtc ctggtggccg ctgctcgagg tgacggaaga agttttcgac gaaatcctgc
     6901 aggtcaacct caaggccgcg atgttcctcg cccaggcagc cgccaggcac cagatcgcgg
     6961 ccgggcgcgg cggcgcgcaa atccatctgc t
//
LOCUS       DASWIY010000203         3513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1608762, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000203 DASWIY010000000
VERSION     DASWIY010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608762"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..132
                     /locus_tag="VGA59_07375"
     tRNA            57..132
                     /locus_tag="VGA59_07375"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:90..92,aa:Trp,seq:cca)
     gene            238..582
                     /gene="secE"
                     /locus_tag="VGA59_07380"
     CDS             238..582
                     /gene="secE"
                     /locus_tag="VGA59_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX9719538.1"
                     /translation="MADKIRITAALLLVAAGVAGFYILDQSPMILRVAAVLAGLAAGA
                     GVFWTSEPGKQFHVFAQESVGETKKVVWPTRKETLQTTGIVFAFMVVMALFLWLVDAG
                     LLWAVKRLLGQS"
     gene            582..1118
                     /gene="nusG"
                     /locus_tag="VGA59_07385"
     CDS             582..1118
                     /gene="nusG"
                     /locus_tag="VGA59_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX9719539.1"
                     /translation="MSGKKWYVVHAYSGFEKSVQRALQDRIRRAGMENKFGQILVPVE
                     EVVEMKGGQKAISERKFFPGYVLVEMEMSDDTWHLVKNTAKVTGFVGGTATKPTPISE
                     KEVQNILHQIQEGVEKPRPKVLFEVGEAVRVKDGPFADFHGNVEDVNYDKNKLRVSVT
                     IFGRSTPVELDFSQVEKA"
     gene            1137..1568
                     /gene="rplK"
                     /locus_tag="VGA59_07390"
     CDS             1137..1568
                     /gene="rplK"
                     /locus_tag="VGA59_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX9719540.1"
                     /translation="MAKKIVGIIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQKLEPGLPVPVVVTAYQDKSFTFVMKTPPASVLIRKAAKIEKGSARPNSEKVGKIT
                     RAQAEEIARAKMPDLTAADLDAAVRTIAGSARSMGVDVEGL"
     gene            1569..2258
                     /gene="rplA"
                     /locus_tag="VGA59_07395"
     CDS             1569..2258
                     /gene="rplA"
                     /locus_tag="VGA59_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX9719541.1"
                     /translation="MASKRYNAMRAKVDRDKSYPAQQALKMVKEGATAKFNESVDVSL
                     NLGIDASKSDQTVRGTVVLPQGTGKSVRIAVFAQGDKAQQAKDAGADIVGFEDLANDI
                     KGGKMEFDVVIASPDAMRVVGQLGQILGPRGLMPNPKVGTVTQDVAGAIKNARAGQIQ
                     YRADKAGIVQCTIGRASFSVDALVENLKSLVDAVNKSKPSSVKGIFLKKVSVSSTMGV
                     GVRVDQASLQQ"
     gene            2590..3120
                     /gene="rplJ"
                     /locus_tag="VGA59_07400"
     CDS             2590..3120
                     /gene="rplJ"
                     /locus_tag="VGA59_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX9719542.1"
                     /translation="MSLNLEQKQAVVAEVSAQLAGAQAVILAEYRSIAVKDMTELRKK
                     ARGSGVYLRVLKNTLARRAVAGTPFKGLSEKMIGPLAYGISSDPVAAAKVLQEFARDH
                     DKFVIRAGAMPNVMMSAREVAELARLPSRQELLAKLVATVQAPIAKFVRTLNEVPGKF
                     ARMLAAVRDQKQQQAA"
     gene            3179..>3513
                     /gene="rplL"
                     /locus_tag="VGA59_07405"
     CDS             3179..>3513
                     /gene="rplL"
                     /locus_tag="VGA59_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX9719543.1"
                     /translation="MAIAKAEILDAIANMTVLELSQLIKEMEDKFGVSAAAATVAVAA
                     PAGGGAAAAAAEEKTEFTVMLNSSGANKVNVIKVVRAVTGLGLKEAKDLVDGAPKAVK
                     EAIAKADAEA"
BASE COUNT          761 a          975 c         1204 g          573 t
ORIGIN      
        1 gtcaatgcga aatccgtaac acggaactca aaactcgaaa ctcgaaactg aatcataggc
       61 cagtagctca attggcagag cgtcggtctc caaaaccgaa ggttgggggt tcgagaccct
      121 cctggcctgc caacgaatga gttacgagtt gcgagtttcg ggttacgagt tgataggcaa
      181 acaagtccgt accatgcacc caactggaaa ctggaaacgg gaaactcgaa actgagcatg
      241 gcggacaaga tcagaatcac cgcggcgctg ttactggttg cggcaggcgt ggcggggttc
      301 tatatcctgg atcagagccc gatgatcctc cgagtcgccg cggtgctggc gggcctggcg
      361 gcgggagcgg gggtgttctg gacctcggag ccgggcaagc agttccacgt gtttgcccag
      421 gagtcggtgg gcgagacgaa gaaggtggtg tggccgacgc gcaaggaaac gctgcagacc
      481 accggcatcg tcttcgcgtt catggtggtg atggcgctgt ttttatggct ggtcgacgcg
      541 gggttactgt gggcggtgaa aaggctgtta ggccagagct gatgtcgggc aagaagtggt
      601 acgtcgttca cgcctactcg gggttcgaga agagcgtgca acgcgcgctg caggaccgca
      661 ttcgccgcgc cggcatggaa aacaagttcg gccagatcct ggtgccggtc gaggaagtcg
      721 ttgagatgaa gggcggacag aaagcgatca gcgagcgcaa gttcttcccc ggctacgtgc
      781 tggtggagat ggagatgtcg gatgacacct ggcacctggt gaagaacacg gccaaggtga
      841 ccggtttcgt cggcggcacc gcgaccaagc ccacgccgat ctcggagaag gaggtgcaga
      901 acatcctgca ccagatccag gagggcgtgg agaagccccg gccgaaggtg ctgttcgagg
      961 tgggcgaggc ggtgcgggtc aaggatggcc cgttcgcgga cttccacggc aacgtcgagg
     1021 acgtcaatta cgacaagaac aagctgcgcg tgtcggtgac gattttcggg cgctccaccc
     1081 cggttgaact ggatttcagc caggtcgaga aggcctgacg gcaggattga ggacttatgg
     1141 caaagaagat agttggcatc atcaagctgc aggtgccggc gggcaaggcg aaccccagtc
     1201 cgccgatcgg cccggcgctc ggccagcgcg gcttgaacat catggagttc tgcaaggcgt
     1261 tcaacgccca gacgcagaag ctggagccgg gcctgccggt gccggtggtc gtcaccgcct
     1321 accaggacaa gagcttcacc ttcgtgatga agacgccgcc ggcaagcgtg ctgatccgga
     1381 aggcggccaa gatcgagaag ggcagcgccc gccccaattc cgaaaaggtg gggaagatca
     1441 cgcgcgccca ggccgaggaa atcgccaggg cgaagatgcc cgacctcacc gctgcggacc
     1501 tcgatgcggc ggtgcgcacc atcgccggca gcgcccgcag catgggcgtc gacgtggagg
     1561 gcctgtaaat ggcttccaag cgctacaacg cgatgcgcgc caaggttgac cgcgacaagt
     1621 cctacccggc gcagcaggcg ctgaagatgg tgaaggaagg cgccaccgcg aagttcaacg
     1681 agtcggtgga tgtttccctc aatcttggga ttgacgcctc gaagtccgac cagacggtgc
     1741 gcggtacggt cgtgctgccg cagggcaccg gcaagagcgt gcggatcgcg gtattcgcgc
     1801 agggtgacaa ggcccagcag gcaaaggacg ccggcgccga cattgtcggc ttcgaggacc
     1861 tcgccaatga catcaagggc gggaaaatgg agttcgatgt ggtaatcgcc tctccggacg
     1921 cgatgcgcgt ggtggggcag ctgggccaga tcctcggtcc gcgcgggctc atgccaaacc
     1981 ccaaggtggg gacggttacc caggacgtgg ccggcgcgat caagaacgcc agggccggcc
     2041 agatccagta ccgcgctgac aaggctggca tcgtccagtg cacgatcggc cgcgcgtcct
     2101 tctcggtgga cgcgctcgtc gagaacctta agtccctggt ggacgccgtg aacaagtcca
     2161 agccctccag cgtgaaaggg atctttctca agaaggtctc ggtgtcgagc acgatgggcg
     2221 tgggcgtgcg cgtggaccag gcgagtttgc agcagtgaag gctttgggcc gccggaggcg
     2281 caccgccggc gggttgtcaa agaccgtagg aatcgcgagg tttaattttt cgggaattag
     2341 attgaaggct caaggatcag aagctgtctc aaagatgctc aatcctcaat cctcaatcct
     2401 caatcctcat tcctgagggt cctacgcaga cggcgtgccc gaaacaggtt ttgtgtctga
     2461 ctcctgaatc aacggtcgcc gtaaaggcag tttcgagttg cgagttacga gtttcgagtt
     2521 gatggtacaa acgcgaaacc cagaacccga aacccggaac acggaacgga ttttcaaagg
     2581 agaaagacct tgagtctaaa tctggagcag aagcaggcgg tggtggccga ggtgagcgcg
     2641 cagctcgccg gggcccaggc cgtgatactg gccgagtacc gcagcatcgc ggtgaaggac
     2701 atgacggagc tgcgcaagaa agcgcgcggc tccggcgtct acctgcgggt gctgaagaat
     2761 accctggcac gccgcgcggt cgcgggcacg ccgttcaagg ggctctccga gaagatgatc
     2821 ggcccccttg cctacggcat ctccagcgat ccggtggccg cggccaaggt gctgcaggaa
     2881 ttcgccaggg atcacgataa gttcgtgatc agggccggcg ctatgcccaa cgtgatgatg
     2941 tcggccaggg aagtggcgga gctcgcaaga ttgccgagcc gccaggaact gctcgcgaag
     3001 ctcgtggcga ccgtgcaggc gccgattgcg aagttcgtgc gcaccttgaa cgaggtgccg
     3061 ggcaagtttg cgcggatgct ggcggcggtc agggaccaaa aacagcagca ggccgcgtaa
     3121 cgcccgctgg accggaattt cagaacactt aacattagat ccgacaggag caagcaacat
     3181 ggctattgcc aaagcagaga ttctcgatgc gatcgcgaac atgacggtgc tggaactctc
     3241 ccagctcatc aaggagatgg aagacaagtt cggcgtttcc gccgccgcgg cgacggtggc
     3301 ggtggcggcg ccggccggcg gcggcgcggc ggccgcggca gccgaggaga agaccgagtt
     3361 cacggtcatg ctcaacagct cgggagccaa caaggtcaac gtcatcaaag tggtgcgcgc
     3421 tgtgaccggg ctcggcctga aggaggccaa ggacctggtg gacggcgctc cgaaggcggt
     3481 caaggaagcc atcgccaagg ccgacgcgga agc
//
LOCUS       DASWIY010000204         3527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1222255, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000204 DASWIY010000000
VERSION     DASWIY010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222255"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VGA59_07410"
     CDS             complement(<1..691)
                     /locus_tag="VGA59_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEX9719544.1"
                     /translation="MSPMVVNNVAYRKGQRLGDVTIEDISEVIIEPDTFVWLGLHEPD
                     EALLLQIKEEFSLHELAIEDAHHAHQRPKIEAYGGSVFIVLKTAQLEDGKVTYGETHL
                     FVGTNFLVSVRHGASSSYRHVRERTELNPRMLAKGPGFAAYAILDFVVDNFQPVVAQF
                     ETTFNSLEEDIFRQQFDRLVIQRLYDLKSDLLELRNTALPVSDISGQLMRFHDDIIHK
                     EIRPYLRDVQDH"
     gene            complement(697..1713)
                     /gene="hemB"
                     /locus_tag="VGA59_07415"
     CDS             complement(697..1713)
                     /gene="hemB"
                     /locus_tag="VGA59_07415"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX9719545.1"
                     /translation="MSLHAPYPQGRPRRLRRDEFTRNLVRENNLTTHDLIYPVFLLDG
                     RQRREAVASMPGVERLSLDLLLPVAEQCVAHGIPVMALFPVIDPALKTPDGQEACNPQ
                     GLVPRAVRELKQRFPDLGVMTDVALDPFTSHGQDGLLDDTGYILNDETVQVLVRQALT
                     QAEAGVDLVAPSDMMDGRIGAIRSALEARGLIHTRIMAYSAKYASAFYGPFRDAVGSA
                     SNLGKSNKKVYQMDPGNTDEALREVAMDIAEGADMVMVKPGMPYLDVVRRVKDEFRVP
                     TFVYQVSGEYAMLKAAARNGWLDHDAVMMESLLAFKRAGADGVLTYFALDAARLLRRD
                     HGAA"
     gene            2059..3186
                     /locus_tag="VGA59_07420"
     CDS             2059..3186
                     /locus_tag="VGA59_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX9719546.1"
                     /translation="MHKTSAWPLSQAYVALIVYASLYPFSGWRNQGIAPWEFLWSPLP
                     KYWTGFDVAANVAGYVPLGFLLALSFLRRGKERFAPTTNTAAITVATLSAAVLALWME
                     AIQSYLPSRVASNVDFGLNVAGALLGAVLAGGLELAGAIDRWSRFRVRWFVEDSRGAL
                     VLLALWPFALLFPASVPLGLGQVFERLETALADWLLDTPFLEWLPVRDVELQPLVPGA
                     EMICVALGAIIPCLLGYSIIQSIGRRVVFGAAAVAVGIAATALSAALSWGPSHAWAWL
                     SLPVRMGLTVGLLLALVLLAIPRRGCAALVLLALVVHLSLLNQAPESAYFAHTLQTWE
                     QGRFIRFHGLAQWLGWLWPYAALAYVLVRVSRSDAQNRMPA"
     gene            3183..3521
                     /locus_tag="VGA59_07425"
     CDS             3183..3521
                     /locus_tag="VGA59_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX9719547.1"
                     /translation="MSDANSYYGRHIFFCLNQRGNGEACCADHHAQEAYDHCKAKVKS
                     LELNVPGKVRVNKAGCMDRCAAGPVAVVYPEAVWYTYVDQHDIDEIVESHLKNGQVVQ
                     RLLTPPHLGR"
BASE COUNT          532 a         1120 c         1162 g          713 t
ORIGIN      
        1 cgtggtcctg gacgtcgcgc aggtaaggcc ggatttcctt gtgaatgatg tcgtcgtgaa
       61 atcgcatcaa ctggccgctg atatcgctta ccggcaacgc ggtgttgcgc agctcgagaa
      121 gatcgctctt caggtcgtag agcctttgga tgaccagccg gtcgaactgc tgcctgaaaa
      181 tgtcctcctc cagcgagttg aaggttgttt cgaactgggc gacgacaggc tggaagttgt
      241 ccacgacgaa gtccaggatc gcataagcgg cgaagccggg gccttttgcg agcatcctgg
      301 gattgagctc ggttcgttcg cgcacgtgcc ggtagctcga ggatgcgcca tgcctgaccg
      361 acacgaggaa attcgtgccc acgaaaaggt gcgtctcgcc ataggtcacc ttcccgtcct
      421 ccagctgcgc tgttttcagg acaatgaaga ccgagccgcc gtaagcctcg attttcgggc
      481 gctgatgcgc gtggtgggca tcttcgatcg ccagctcgtg gaggctgaat tcctccttga
      541 tctgcaacag cagcgcctcg tcgggttcgt gcaggccgag ccagacgaag gtgtccggct
      601 caatgatgac ctcgctgatg tcctcgatgg tgacgtcgcc caggcgttga ccctttcgat
      661 aggcaacgtt gttgacgacc atcggcgaca tgggcatcat gccgcgccgt ggtcccggcg
      721 cagcagcctc gcggcgtcca gcgcgaagta ggtcagcacc ccgtcggcgc cggcgcgctt
      781 gaacgcgagc aggctctcca tcatcacggc gtcgtggtcg agccagccgt tgcgcgccgc
      841 cgccttgagc atcgcgtatt cgccgctgac ctggtacacg aacgtcggca cccggaattc
      901 gtccttcacg cgccgcacca cgtcaaggta gggcatgccg ggcttgacca tcaccatgtc
      961 tgcgccttcg gcgatgtcca tcgcgacttc gcgcagggcc tcgtcggtgt tgcccggatc
     1021 catctggtag acctttttgt tgctcttgcc caggttcgaa gcggaaccca ccgcgtcgcg
     1081 gaacggcccg tagaaggcgc tggcgtattt ggcgctgtac gccatgatcc gcgtgtggat
     1141 cagcccgcgc gcttccagcg ccgagcggat cgcgccgatg cgcccgtcca tcatgtcgct
     1201 gggcgcgacc aggtcgacgc cggcctcggc ctgggtcagc gcctggcgca ccaggacctg
     1261 caccgtttca tcgttgagga tgtagccggt gtcgtcgagc aggccgtcct gcccgtggct
     1321 ggtgaacgga tccagcgcca cgtcggtcat gacgccgagg tccggaaacc gctgcttgag
     1381 ttcgcgcacc gcgcgcggca ccagcccctg cggattgcag gcttcctggc cgtccggcgt
     1441 tttcagcgcg gggtcgatga ccggaaacaa ggccatgacg ggaatgccat gcgcgacgca
     1501 ttgctcggcc accggcagca gcaggtcgag gctcagccgt tcgacaccgg gcatcgacgc
     1561 gaccgcttcg cgccgctggc ggccgtcgag caggaaaacc ggatagatca ggtcgtgcgt
     1621 ggtcaggttg ttttcccgca ccaggttgcg ggtgaattcg tcgcggcgca gacggcgcgg
     1681 acggccttgg gggtagggtg catgcaggct catgctggaa attgtgctcc atcccgacgg
     1741 ccggctcacg gcgctgccgt cttgtggcgc ttatgcatcg gcaggtgacg ccaagtcgat
     1801 gattcatatg ggttctagcc gcagggcgga taaaagcttc gcgccgccct tgaaagcgac
     1861 cgcccggttt gttaaattac ggctgggcgg ttcgccgctc cccgcttttc ctccctgagc
     1921 gggtgcctta atgtgtgaca gcaaaaaggc ttcccacccc ggctttgagc cggggttttt
     1981 tttggtgcgc ccggtttgcg cgggcgcggc cggcagtggc acggcccgga cttcttttgt
     2041 ccctacactt cgggcagcat gcacaagact tcggcctggc cgctctcgca agcctatgtg
     2101 gctttgatcg tctacgccag cctgtacccg ttttctggct ggcgcaatca ggggattgct
     2161 ccctgggagt tcctctggag cccgttgccc aagtactgga ccggcttcga cgtggccgcc
     2221 aacgtggcgg gctacgtgcc cctgggcttc ttgctcgccc tgagcttcct gcgccgcggc
     2281 aaggagcgct tcgctcccac cacgaacacg gcggcgatca cggtggccac gctctcggca
     2341 gcggtcctgg cattgtggat ggaggccatc caaagctacc tgccttcgcg ggtcgcctcc
     2401 aacgtggatt tcggcttgaa tgtggcgggc gctttgctgg gcgcggtgct ggccggcgga
     2461 ctggaactgg ccggcgccat cgatcgctgg agccgctttc gggtgcgctg gttcgtggag
     2521 gactcgcgcg gcgccctggt gctgctcgcg ctatggccgt tcgcgctgct gtttccggca
     2581 tcggtcccgc tggggctggg ccaggtcttc gagcgcctcg agacggccct ggccgattgg
     2641 ctgctcgata ctcccttcct ggaatggctg ccggtgcgcg acgtcgaatt gcagccgctg
     2701 gttccgggcg ctgaaatgat ctgcgtcgcc ttgggcgcca tcattccatg cctgctgggc
     2761 tactccatca ttcagtcgat cggccggcgt gtcgtgtttg gcgcggcagc cgtggccgtc
     2821 ggcatcgctg ccacggccct gtcggcggcc ctgagctggg gtccgagcca cgcctgggcc
     2881 tggctgagcc tgccggtgcg catgggtttg acggtggggc tgctgctggc gctggtgctg
     2941 ctggcgattc cgcgccgcgg ctgcgccgct ctggtactgc tggcgctggt ggtccacctc
     3001 agcttgctga accaggcgcc ggaaagcgcc tacttcgcgc acaccctgca aacctgggag
     3061 caaggccgct tcatccgctt tcatgggctg gcccagtggc tgggctggct gtggccttat
     3121 gccgccctgg cttatgttct ggtgcgcgtg tcgcgaagcg acgcgcaaaa tagaatgccc
     3181 gcatgagcga tgcgaattcc tattacgggc ggcacatctt tttttgcctg aaccagcgcg
     3241 gcaacggcga ggcctgctgc gccgatcacc acgcccagga ggcctacgac cactgcaaag
     3301 ccaaagtcaa gtccctggaa ttgaacgtcc cgggcaaggt gcgcgtgaac aaagcgggtt
     3361 gcatggaccg ttgtgccgcc ggcccggtgg ccgtggtcta tccggaggca gtctggtaca
     3421 cctacgtgga ccagcacgac atcgacgaga tcgtcgagtc gcacctgaag aacggccagg
     3481 tcgtgcagcg gctgctgacg ccgccgcacc tgggacgatg aattccc
//
LOCUS       DASWIY010000205        16761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2357898, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000205 DASWIY010000000
VERSION     DASWIY010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 16761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16761
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2357898"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VGA59_07430"
     CDS             complement(<1..643)
                     /locus_tag="VGA59_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9719548.1"
                     /translation="MHPLAKAFAGAGYEAYALDIRGHGASGTKGTIDYVGQLEDDLTS
                     FMRAVSVARPATLAGFSSGGGFVLRFAGSQRQDEFQNYLLLSPFLNPDAPNSRPGSGG
                     WVDVGIPRYIGLAVLNSLGIHTFKDLPVTSFALTEEAKTFLTPAYSFSLAANFQPQRD
                     YEANIRAVHQPMAIVAGASDEAFQTDKLEGIFRRQGQPWPVTLLPGIGHIPLTL"
     gene            complement(823..1044)
                     /locus_tag="VGA59_07435"
     CDS             complement(823..1044)
                     /locus_tag="VGA59_07435"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9719549.1"
                     /translation="MPKLPRISGAEAQRALERLGFVKLRQSGSHVIMRRESKGCVVPM
                     HAELKVGTLAGILRQADVAPEEFIAALKQ"
     gene            complement(1037..1249)
                     /locus_tag="VGA59_07440"
     CDS             complement(1037..1249)
                     /locus_tag="VGA59_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719550.1"
                     /translation="MELTAVLTPAEEGGFVALNPETGTTTQGETVEEAVANLREATAL
                     YLSEFPLPAGGHPVVTVFTVPTLGHA"
     gene            complement(1380..2336)
                     /locus_tag="VGA59_07445"
     CDS             complement(1380..2336)
                     /locus_tag="VGA59_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX9719551.1"
                     /translation="MNGIDALLAVIVLLSALMGSRRGFVVAALQLLTLGSSLVVAFLG
                     YGYLAGWLQAEAPSLGVWALPLSFVAIFIVCHLVLGAVAGVLVGAVPPSVHAAGANKA
                     LGVLPGVVNGLINATVVSLFVLATPLLDGLTASARDSEFANRLSGPAEWLEAKLAPIF
                     DPAVRRTLQALTVQPESRTTVPLRFRVADPKVRTDLEARMLEMVNAERAGRGLKPLRA
                     DPELAQVARAHSRDMLERGYFSHVAPDGKDAFDRMRKANVRFLMAGENLALAPTLPGA
                     HQGLMTSPGHRANLLRPQFGRLGVGVLDAGKYGLMITQNFRN"
     gene            2689..3309
                     /locus_tag="VGA59_07450"
     CDS             2689..3309
                     /locus_tag="VGA59_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719552.1"
                     /translation="MSALRKVLVVDDDPVVGMSFKRVLSGKGYVVITAENGYEGLAKL
                     QDEKFDLVFTDLRMPGLTGLDVAERVKARQPWMPVVIVTGYGSKTSEDRARAAGVSGF
                     LNKPLSPEMIEESTERALSGPAPTAVMQPEVQAAAPAAHAEPEPEKKRNLFVNMALFL
                     SAPFIGLLYAVLLPFVGMGMLAWAAFKAIRAPKQAAQEPDSPVAIE"
     gene            3337..3672
                     /locus_tag="VGA59_07455"
     CDS             3337..3672
                     /locus_tag="VGA59_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9719553.1"
                     /translation="MKVSPVLAAAAAVFAFSMPAAAAVDADAAVKLSKQSGCTKCHSV
                     DKAKKGPAFQKVAAKYKGKADGEARLIEFVTKSPKVKLDDGSEEEHKAVSTKDAAQVK
                     NLVQWMLAQ"
     gene            complement(3714..4487)
                     /locus_tag="VGA59_07460"
     CDS             complement(3714..4487)
                     /locus_tag="VGA59_07460"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEX9719554.1"
                     /translation="MSAFNEERVLSVHHWTDRLFTFKTTRDQSLRFSNGHFTMIGLRV
                     NDKPLLRAYSIVSANYEEHLEFLSIKVPEGPLTSRLQHIKVGDTIIVGRKPTGTLLID
                     YLMPGKRLFLLSTGTGLAPFMSIVHDPATYEKFEQIILVHGVRQKDELAYHDLLVEHL
                     PAHEFLGELVSSQLRYYPTVTREAYRNMGRVTDLMHTGKMFEDLGIEPLNQAGDRVMI
                     CGSPGMLRDLKHMLESQGFVEGNTSRPGDFVIERAFAEQ"
     gene            4696..5349
                     /locus_tag="VGA59_07465"
     CDS             4696..5349
                     /locus_tag="VGA59_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX9719555.1"
                     /translation="MRIVLAAFLLAFLVAYGAVAETADPGSDAPEGPRHVAAFKDAAS
                     YPQALQVWRTPEDVNAWIGAKFHYDMSRAMVLSESQRSRSGQVSIYRAEEFFATPSGI
                     CVDLSRFAVETLRQIDPELKANYLMIEFSPVAIGGNTLRMHWLVSFTRDGKYYFFADS
                     KRPGHISGPHASVQEFVAGYAEYRNRQVVAFRELDSYQRKQRAMASTRRAPEPERYP"
     gene            complement(5428..5823)
                     /locus_tag="VGA59_07470"
     CDS             complement(5428..5823)
                     /locus_tag="VGA59_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX9719556.1"
                     /translation="MQDKPMKVPGPEHPITIEANPARVVVTVAGRVIADSVGALTLRE
                     AGYPPVQYIPRKDVDMTLLQRTDRATYCPFKGDCAYYSIPSGGERSVNAVWTYEAPYP
                     AVAEIKDRLAFYRHRVDSIEEWPGEPGAG"
     gene            complement(5828..7420)
                     /locus_tag="VGA59_07475"
     CDS             complement(5828..7420)
                     /locus_tag="VGA59_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX9719557.1"
                     /translation="MSHIGKPPCDEGAVLYGAEPSPCPQSARRWTLVAAILGSSMAFL
                     DGTVVNVALPAIQRDLDASASQAQWVIESYALFLAALLLAGGALGDRLGQRRVFMAGV
                     ALFTVAAAGCAFAGTAGQLIAARAVQGVGAALLVPGSLALISTAFPQAQRGQAIGTWS
                     AFSGIAAAVGPVLGGFLVDHYSWTWAFLINLPLGAALLALCVAKVPEHRAAQGQQAGL
                     DIWGAALATVGLAGIVFAFIEAPNLGWTAPAVLGAALVGVLAIAMFVVAEARSPSPML
                     PLALLRIRNFAGANLLTLLLYAALGGGLYFFPLNLIQVQGYGAAAAGAALLPFIVIMF
                     LLSRWAGTLVDRFGPRLPLIAGPLIAAAGFALFALPAVGGSYWSTFLPAVSVLGFGMA
                     VTVAPLTTTVMNSVGPELAGLASGVNNAVSRAGALLALAVFGVVMSAAFDPALDRRIS
                     ALDLPPAVQRGIEEQRPRLAAMTVPSGVDPTTASAIQRAVGESFVAGFRRVMLVCAAL
                     ALLSALSTWVLIGGESRRETRG"
     gene            complement(7515..8282)
                     /locus_tag="VGA59_07480"
     CDS             complement(7515..8282)
                     /locus_tag="VGA59_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX9719558.1"
                     /translation="MDSATTSPVPNLNAVTEQQVRVDLAACYRLVAAYGWDDLIFTHI
                     SARVPGPEHHFLINPYGMMFGEITASSLVKVDLQGRKVDGSPHEINPAGFTIHSAVHA
                     ARDDAQCVLHVHSVNGVAVSAQEGGMLPLSQHSIFVLASLAYHDYEGVALEDDEKPRL
                     VRDLGTRRFLMLRNHGLLTMGRSVAEAFVAMYFFETACMIQVRALSGGGPVRRIGSRI
                     LEGADQQWDMVTRGAGGGLAWPALLRKLDSMNPGYRD"
     gene            8343..8921
                     /locus_tag="VGA59_07485"
     CDS             8343..8921
                     /locus_tag="VGA59_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9719559.1"
                     /translation="MISDTQSLSSRDRMIEAAIDLMRGYGLSGVGINDVIRESGAPRG
                     SLYHFFPDGKIQIASEALAVQGGRISSFIEKALSSKKAGPQKLLALFEAFARRVEESE
                     FQKSCAVGAVTLDLAADAEPLRLVLAGIFEAWRGVIARHFQMSGPREAESFAGLVLTA
                     IEGAHIRARAERSGAPFREAGHWLSRLVKQAS"
     gene            complement(8931..9776)
                     /locus_tag="VGA59_07490"
     CDS             complement(8931..9776)
                     /locus_tag="VGA59_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX9719560.1"
                     /translation="MKLVTWNAQWCRGLDGVVNPERIVKDARRLADFDVLCLQEIAVH
                     YPRLPGNAGHDQPALLRELLPGFQVFFGAAVDEWGKDGHRSQFGNLVATRLAVAQVQH
                     HPLPYPAHGGMWSMPRMCTVVTMIDPGLGPVRVMTAHLEYFSEPQRVAQARALRELHL
                     QACAHTAAPPKSNEDGSPFQTKVHTENAILCGDFNLESHQPGYRAITRPFAQGRLWDC
                     WRLLNGRAPQPHTFRLHARKPGEKPMACDFVFVGESLKGRVRGLQIDSATQASDHQPL
                     ALELG"
     gene            complement(9784..10578)
                     /locus_tag="VGA59_07495"
     CDS             complement(9784..10578)
                     /locus_tag="VGA59_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEX9719561.1"
                     /translation="MNDSPDPPASDSPAAPELAPLGSDDFDAQDAELDLLREDDEEIP
                     QWEFCEGFLAALVCARRAIAPEEYWPVLLGDGFKPVAHMEFVWRWRRRWAEVAAALDA
                     PAESLDDERTYQPEVLDTRGAVLALPQDERGDTDIATLPSYAQVWALGFMYAVETWPE
                     DWAAPRDREAAEMLDNALGAIVALTGDDGGKPSVSMFAEDGPPSVSEQRLDDYGAAIW
                     AVYDLRQLWKSLGPRTEAVRKAPEPGRNDPCPCGSGKKYKKCHGAG"
     gene            complement(10625..12034)
                     /locus_tag="VGA59_07500"
     CDS             complement(10625..12034)
                     /locus_tag="VGA59_07500"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HEX9719562.1"
                     /translation="MNDIAELLTGTLARQRQAYLAHPVPSLDERKADLRTLQRFVRDH
                     KTAICDAISADYGHRSRHETLLAEIFPAIDGIDHVIKQLRRWMKPQRRKVDLRNFFGA
                     RNRVIPQPLGVVGVIVPWNFPLNLSVIPLTYIFAAGNRAMVKMSENSRHLAQLLIERM
                     PAYFPPEKLQFFDETGGVGVEFSRLPFDHLLFTGSGATGRAVMTAAAQNLCPVTLELG
                     GKAPAILCDDFPLRLAAERILFVKYLNAGQICTTVDHLWMPKAHIAEFVELAREIVPK
                     RFPQLDTPDYTSIIDRRSFDRLLTDLDDARERGATLIQLVPGPAYDRATRKIAPHLVL
                     DAPLESALWQREIFGPILPIRGYQSLDEAVNAINAGPRPLALYPFSNSRSTVENLLER
                     VMSGGVSVNDGLFHVGQHDLPFGGVGDSGMGHYHGEEGFHTFSKLRPVFYQARFSTLK
                     YLMPPYGKFADRVLAFLTK"
     gene            12416..12622
                     /locus_tag="VGA59_07505"
     CDS             12416..12622
                     /locus_tag="VGA59_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719563.1"
                     /translation="MIDSTPNQFELRFQSLFDSGRGYAFPCDPKGLVDLDQLSDRSRN
                     NYLYARAMVGRELAVPAVRPSGMH"
     gene            complement(12645..14039)
                     /locus_tag="VGA59_07510"
     CDS             complement(12645..14039)
                     /locus_tag="VGA59_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719564.1"
                     /translation="MHEPAIEVDYLIKGAGAAGMAFADSVVSDSTATIAIVDRHHRPG
                     GHWNHAYPFVRLHQPSAFYGVNSRPLGSGAKDRIGLNKGFYELASGSEVLSYFDLVMR
                     QGLLPTGRVRYFPMSELGDDGTVTSLLSGEQCTIRARKIVDATYSNTVVPSMRPAPYP
                     VDPDIVCVPLNDLPRIARPHAAYVVIGAGKTGMDACLWLLENGADPDRVRWIMPRDYW
                     WINRATYQPGDEFTARAIQSIANGVEALAGGESVDDVFARLEAFGEVRRIDPSVKPEG
                     YHGGLVSDGELAQLRRIRDVVRLGRVTRIESDRIVLARGVVPTSRDCLHIDCSATGIP
                     ARPSKPIFAGDHIALQWVRLAQPTFSWSLIGHVEATCQDEAEKNRICAPIAPPDEPRD
                     WVRMMGAELSNQLAWSKIPDIREWQAKSRLDPFTRRIRSLKGTDGEPMAHLQRYLKYV
                     GPAAQKIPRLLGAA"
     gene            14153..15040
                     /locus_tag="VGA59_07515"
     CDS             14153..15040
                     /locus_tag="VGA59_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9719565.1"
                     /translation="MHTRHFLQLIFLSALWGGSFPLIRIASPAFGPLGLAGIRCVLAA
                     VVLALLMRVLREHWPRTALTWRRLTVLSLLTVVSPFVLFNWAGLVLPAGYSAVLNATA
                     PLFGILGAAAIGEERLTTRRLAGCALGFAGVALLVRLGPVAVDARVVLAALACTAAAA
                     SYGLGAIMMKRATLAHHPLPASAAVHVAASLVLLLPTAATATRMQPTTGALIAVAILG
                     TVTSGFMYWVSMRLMREISASAATSAAFMIPLFGVTWGGLFLDEPVTAGMLPGCVLVL
                     AATALVTGFNPFKAVPPEP"
     gene            complement(15392..15898)
                     /locus_tag="VGA59_07520"
     CDS             complement(15392..15898)
                     /locus_tag="VGA59_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9719566.1"
                     /translation="MNRRHTLLLAVSVLALASGGAVHAQAAPAPVEKELREALRNFEQ
                     AWNRHDVKAWSAHLTEDVWFTQAWDYYAKQKGRGNAVDLFKSNFLDTDFTHQVVRIKM
                     MPDGTATVAKTIAVSYLPKTDGKYRMVFESNPVISRWKKEGSTWKMFFFTSHEGWARD
                     QLKKDGIE"
     gene            complement(16096..>16761)
                     /locus_tag="VGA59_07525"
     CDS             complement(16096..>16761)
                     /locus_tag="VGA59_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9719567.1"
                     /translation="DLHLAVAHLRDLATLRVGKVAVAALPSVAAVWMPQVIARYRAAN
                     PMIRVQLFDTLAQGSVDLLRAGTVDLAVTAGGDLREFELIELKRERFYLVCPATHSLA
                     GKRTVSLSQLAGEEIVHLSPSSSVRRCLDAAQAEPLGKPGGLEAEHLATVAGLVAQGL
                     GVSVVPELTLFHFRREGLATIPVRNTNLVRPVFLARRKGKALSIAAQAMSEEIVAQAR
                     APR"
BASE COUNT         2921 a         5446 c         5525 g         2869 t
ORIGIN      
        1 caagggtgag cgggatgtgc ccgattccgg gtagcagtgt caccggccag ggttgacctt
       61 gcctgcgaaa gatgccctcg agcttgtccg tctggaaagc ctcatcgctc gcccccgcca
      121 ctattgccat tggctggtga accgccctga tgttcgcctc gtagtctcgc tggggctgaa
      181 aattcgcggc caaggagaat gagtacgcgg gggttaaaaa ggttttcgct tcctcggtga
      241 gcgcgaaact ggtaaccgga agatccttga aagtatgaat gcccaggcta ttcaacacag
      301 ccaggccgat gtagcgagga atgccgacat cgacccagcc gccgctgcca ggccgagagt
      361 tcggtgcatc ggggttaaga aacggcgaga gcagcaagta gttttggaac tcgtcctgtc
      421 tttggcttcc agcaaaacga agtacaaagc cgccgccgga ggagaatccc gcgagggtag
      481 ccggccttgc caccgacaca gccctcatga acgacgtcaa gtcgtcttcg agctggccga
      541 catagtcgat cgtcccttta gtcccggacg caccgtgccc gcgtatgtcc agcgcatacg
      601 cctcgtagcc agctccggca aacgccttgg caagcgggtg catgctattg ctgcttgcag
      661 acgaaccgtg aacgagaacg atgcttcctt tggtatcgac ccctggcagc acgtagcggc
      721 gataggcgag cccggcacgt cggaaatgac cggcgcacaa ggtagaagtg cgcaacgcgc
      781 acgggcgcac ttgtgcgtcc gtgtcgaagg acttgagaga cctcactgct tcaacgccgc
      841 gatgaactct tcgggcgcca cgtcggcctg ccgaagaata ccagccaggg tgccgacctt
      901 caactctgca tgcatgggca caacgcagcc cttggactcg cgccgcatga tgacgtgact
      961 gccgctttga cggagcttga cgaaccctaa ccgctccaga gctcgctgcg cttctgcgcc
     1021 cgagatccgg ggcagcttag gcatggccga gcgtgggcac ggtaaaaacc gtcacgacag
     1081 ggtggccgcc ggcgggcaac ggaaactccg acaagtacaa cgcggtggcc tcgcggagat
     1141 tggcaacggc ctcttcaacc gtttcaccct gggtggtcgt tcctgtctcc ggattgagcg
     1201 cgacaaagcc accttcctcg gcgggcgtaa gcacagcggt aagttccatt gaggttcgct
     1261 cccattccgg acggccccat tatcgctcca cgccgtcgtg agggggccgg ccgactcggc
     1321 ggcgtccttg gggtcgacga tcgcctgggc ttcggcttcc tgctcccgca tgcgttgcct
     1381 cagttgcgga agttctgcgt gatcatcagc ccgtacttgc cggcatccag cacgcccacg
     1441 ccgaggcggc cgaactgcgg gcgcagcagg ttggcgcggt gacccgggga cgtcatcagg
     1501 ccctggtgag cgccgggcag tgtgggcgcg agggcgaggt tctcgccggc catgaggaaa
     1561 cgcacgttgg ccttgcgcat gcggtcgaaa gcgtccttgc catccggcgc gacgtgcgag
     1621 aagtagccgc gctcgagcat gtcgcggctg tgcgcgcgcg ccacctgcgc cagctcggga
     1681 tcggcccgca gcggcttgag cccgcggccg gcacgctcgg cgttgaccat ctcgagcatg
     1741 cgggcctcca ggtcggtccg gaccttcggg tccgcgaccc ggaagcgcag cggcaccgtc
     1801 gtgcgcgact cgggttgcac ggtgagcgcc tgcaaggtgc gccgcacggc cggatcgaag
     1861 atgggcgcga gcttggcctc cagccactcc gcgggcccgg acagccggtt cgcgaactcg
     1921 ctgtcgcgcg ccgaggcggt caagccgtcg agcagcggtg tcgccagcac gaacaaggac
     1981 acgacggtgg cgttgatcaa gccgttgacc acaccgggca gcacgcccag ggccttgttg
     2041 gccccggcgg cgtgcacgga tggcggaacg gcgccgacaa gcacgccggc aacggcgcca
     2101 agaacgaggt ggcagacgat gaagatggcc acgaagctca gcggcaaggc ccagacgccg
     2161 agcgaaggcg cctcggcctg gagccagccg gcgagatacc cgtaccccag gaaggccacc
     2221 accaggctgg agccgagtgt cagcagttgc agcgcggcca cgacgaagcc cctgcgcgag
     2281 cccatcaagg cactcagcag gacgatcacc gccagcaggg catcgatgcc gttcataaag
     2341 tcagcttata gcttgctcgc aagggagtat ttctcccgca accccagcga acccgtagga
     2401 cgacatcgcc gcttgacgcc gcgccggctg cggggtgtat agaaaaacgg tccccgcttg
     2461 cggccgcaag ctgttgaatc gcaagggttt tttagcaggg gaccgctttc accgcacaaa
     2521 gaaaatgtac ttggagcccg cgccgcgggt gctgcgggca tggcgcgaat gatccaagtc
     2581 cttgattcga aacggcaatt cgtttcgcgg ccaggcctgg cacggccaat gcttcgaaga
     2641 gcacacggct ggctggccga acaacccgat tcattcaagg acattgccat gagcgcactt
     2701 cgcaaggtat tggttgtcga cgacgacccg gtggttggca tgagtttcaa acgtgtgctg
     2761 tccggcaagg gctacgtcgt gatcaccgcc gagaacggct atgaaggact ggcgaagctg
     2821 caggacgaaa aattcgacct tgtcttcacc gatctgagga tgcccggctt gaccggcctg
     2881 gacgtggccg aacgcgtcaa ggcccgccag ccatggatgc cggtggtcat cgtgaccggt
     2941 tacggcagca agacaagcga ggaccgcgcc cgcgccgcgg gcgtgtccgg gttcctgaac
     3001 aagccgctct cgcccgaaat gatcgaggag agcacggagc gcgccctgtc cgggccggcg
     3061 cccacggccg tcatgcagcc cgaggtgcag gcggcggccc cggcggcgca tgccgagccc
     3121 gagccggaga aaaaacgcaa cctgttcgtg aacatggcgc tgttcctctc ggcgcccttc
     3181 atcggcctgc tgtacgccgt gctgctgccc ttcgtgggca tgggcatgct ggcctgggcc
     3241 gccttcaagg cgattcgcgc gcccaagcaa gccgcgcaag aacccgattc gccggttgcg
     3301 atcgagtaat caaccctcat ttacaggagc agatgtatga aagtcagtcc cgtgcttgcg
     3361 gccgccgccg ccgtattcgc cttctcgatg ccggcagcgg ccgcggtcga tgccgacgcc
     3421 gccgtgaagt tgagcaagca aagcggatgc acgaaatgcc actcggtcga caaggccaag
     3481 aaaggcccgg cgttccagaa ggtggccgcc aagtacaagg gcaaggccga tggcgaggcc
     3541 aggctgatcg agttcgtcac caagagtccc aaggtcaagc tcgacgacgg cagcgaggaa
     3601 gagcacaagg ccgtcagtac caaggacgcg gcccaggtga agaacctggt ccagtggatg
     3661 ctcgcgcaat aggtttgctg cagggagggg ccgggccgcg cgcccggcct tatttattgt
     3721 tccgcgaaag cgcgctcgat cacgaagtcg cccggccgcg atgtgttgcc ttcgacgaag
     3781 ccctggcttt ccagcatgtg cttcaagtcg cgcagcatgc ccgggctgcc gcagatcatc
     3841 acccggtccc cggcctggtt cagcggctcg atccccaggt cctcgaacat cttgcccgtg
     3901 tgcatgagat cggtgacgcg gcccatgttg cggtaggcct cgcgcgtgac ggtggggtag
     3961 tagcgcagct ggctgctcac caattcgccc aggaattcat gggcgggcag gtgctcgacc
     4021 agcaggtcgt ggtaggcgag ctcgtccttc tggcgcacgc cgtggaccag gatgatctgc
     4081 tcgaatttct cgtaggtggc cgggtcgtgc acgatgctca tgaacggcgc caggccggtg
     4141 cccgtggaca gcaggaacag gcgcttgccg ggcatcaggt agtcgatcag cagcgtgccg
     4201 gtgggcttgc gccccacgat gatggtgtcg cccaccttga tgtgctgcag gcgcgacgtc
     4261 aggggaccct cgggcacctt gatgctgaga aattccaggt gctcctcgta gttggcgctg
     4321 acgatgctgt aggcgcgcag cagcggcttg tcgttcacgc gcaggccgat catcgtgaag
     4381 tggccgttgg aaaaccgcag cgactggtcc cgcgtggtct tgaaagtgaa caggcggtcg
     4441 gtccagtggt ggacggacag gacacgttct tcgttgaatg cgctcatggg tgggttcggc
     4501 tgtacggtag ggctgaggtg aagcgccaat cttaatcagc attgccggct tcccgcaaaa
     4561 gccccgcgag agcccatgtc cccttcgtgt cacggtttca agttcaaaaa cggccagatt
     4621 gacgctatgc ggggtttatg gcctgattta gttcgggacg gggaagtccc tttcattcgg
     4681 ttaggagccg atcacatgcg catagtcctc gctgcgttcc tgctggcctt cctggtagct
     4741 tacggcgccg ttgcggagac ggcagacccg ggttcagatg ctcctgaagg gccgcgccat
     4801 gtcgccgcat tcaaggacgc agcgtcatat ccacaggcgc tgcaggtgtg gcgaacccca
     4861 gaggacgtca acgcatggat cggcgcgaag ttccactacg acatgtcgag ggcgatggtg
     4921 ctctccgaga gtcagcgaag caggtctggt caggtgtcca tttatcgggc ggaggaattc
     4981 ttcgccactc cctcgggtat ctgcgtcgat ctgagccgct tcgcggtaga gacgcttcgg
     5041 cagatagacc cggaactcaa ggcgaactac ctgatgatcg agttctcgcc ggttgcgata
     5101 ggcggcaaca ctttgcgcat gcactggttg gtgtctttca cgcgcgacgg caagtactac
     5161 ttctttgccg actcgaagcg ccctggtcat atctccggcc cgcatgccag cgtacaagaa
     5221 ttcgtcgcag ggtacgcgga ataccgaaac agacaggtgg tcgccttccg cgagctggat
     5281 tcctaccaac gcaagcaacg cgcaatggcc tcgacgcggc gcgcgcctga accggaacgc
     5341 tatccatgac agcacgcccc gctcggtcga ctcgaccttc tcgtctgaaa aacaactgat
     5401 ggggccggcg tcggagcgcc ggcggcttca gcctgcgcct ggctcgcccg gccactcctc
     5461 gatcgaatcg acccgatggc ggtagaaggc cagtcgatcc ttgatttcgg ccaccgccgg
     5521 gtacggagcc tcataggtcc acaccgcgtt caccgagcgc tcgccgccgg aaggaatgct
     5581 gtagtaggcg cagtcgcctt tgaacggaca gtacgttgca cggtcggtgc gctgcagcag
     5641 cgtcatgtcc acgtccttgc gcgggatgta ctgcacgggc gggtaccccg cctcgcgcag
     5701 ggtcagggct ccgacactgt cggcgataac ccgaccggcg acggtgacga cgacccgggc
     5761 cgggttcgcc tcgatggtga tggggtgttc cgggcccggg accttcatcg gcttgtcttg
     5821 catggtttca tcctcttgtt tcccgccggg actcgccgcc gatcaggacc caggtgctca
     5881 acgcgctcag taacgcgagc gccgcacaca ccagcatcac ccggcgaaag ccagccacga
     5941 acgactcgcc taccgcgcgc tggatcgccg acgccgtcgt cgggtccact cccgaaggca
     6001 cggtcatcgc ggccagccgc ggacgctgtt cctcgattcc gcgctgaacc gccggcggca
     6061 agtccagcgc cgaaatccgg cggtccaatg ccggatcgaa cgccgcgctc atcaccacgc
     6121 cgaatacggc gagggccagc agcgcacccg cccgggagac ggcgttgttg acgcccgatg
     6181 ccagtcctgc cagttcaggg ccgacggagt tcatcaccgt ggtggtcagg ggcgcgacgg
     6241 tcacggccat tccgaatccg agcacgctga ccgccggcag aaatgtcgac cagtaagagc
     6301 cgccgacagc aggcagcgcg aacagggcga aaccggccgc ggcgatcagc ggcccggcga
     6361 tcagcggcag tctcgggccg aatcggtcca ccagcgtccc ggcccagcga gacagcagga
     6421 acatgatgac gatgaagggc agcagcgccg cgccggcggc ggccgccccg tagccctgca
     6481 cctggatgag gttcaacgga aagaagtaca gcccgccgcc cagcgccgca tacagcaaca
     6541 aggtcagcag gttcgcgccg gcgaaattgc ggattcgcag cagcgccagc ggcagcatcg
     6601 gggaggggct tcgcgcctcc gcgacgacaa acatcgcaat cgccagcacc ccgaccagtg
     6661 ccgcgcccag gacggccggc gcagtccagc cgagattcgg tgcctcgatg aaagcgaaga
     6721 cgattccggc tagacccacc gtggccagcg ccgctcccca aatgtccagg cctgcttgct
     6781 gcccctgcgc ggcgcgatgc tccgggactt tggcgacgca cagcgcgagc agcgcagcgc
     6841 cgagcggcag gttgatgagg aaagcccagg tccacgaata atggtcgacg agaaacccgc
     6901 ccagcaccgg gcccacggcg gctgcgatgc cgctgaacgc ggaccaggtg ccgatggctt
     6961 gcccgcgctg cgcctgcgga aaggcggtgc tgatcagcgc gaggcttccc ggcacgagca
     7021 gggctgcgcc cacgccttgc accgcgcggg cggcgatcag ctgcccggcg gttccggcaa
     7081 aggcacagcc ggccgcggca acggtgaaaa gcgccacgcc ggccatgaac acgcggcgct
     7141 gtcccaagcg gtcgcccagt gcgcctcccg ccagcagcaa ggcagcgagg aacagcgcat
     7201 aggactcgat cacccactgc gcttgcgatg cgcttgcgtc gaggtcccgc tggatggcgg
     7261 gcagcgcgac attgacgacg gtgccgtcga ggaaggccat gctcgatccg agaatggcgg
     7321 ccaccagtgt ccagcgccgg gcggactgcg ggcagggcga cggctcggcg ccgtacagca
     7381 cggcgccttc gtcgcaaggg ggtttgccga tgtggctcat gcgggatgct tcgcaggccg
     7441 caaggatacg cccgtgcccc ggcacgttgc tgtggcgccg cggccgaagc tgcggatcgg
     7501 aggacggccg ttcctcagtc gcggtaacct ggattcatcg aatccagctt gcgcaacaag
     7561 gccggccagg cgagcccgcc gccggcgccg cgggtgacca tgtcccattg ctggtcggcg
     7621 ccttcgagga tgcgcgatcc gatccgccgc actgggccgc cgccgctcaa ggcgcgcacc
     7681 tggatcatgc aagcggtctc gaagaagtac atggcgacga aggcttcggc gacggaccgg
     7741 cccatggtca gcaggccgtg gttgcgcagc atcaggaacc gcctggtgcc caggtcacgc
     7801 accagcctcg gcttttcgtc gtcctcgagc gccacgcctt cgtagtcgtg ataggccagg
     7861 gacgcgagaa caaaaatcga gtgctgggaa agcggaagca ttcctccttc ctgggccgac
     7921 accgccacgc cgttgacgct gtgcacgtga aggacgcact gcgcgtcatc gcgtgccgca
     7981 tgcacggcgc tgtggatggt gaaccccgcc ggattgattt catgcggact gccgtccacc
     8041 ttgcgtcctt gaaggtccac cttcaccagg ctggaggccg tgatttcgcc gaacatcatg
     8101 ccgtacgggt tgatcaggaa atggtgctcc gggccaggca cacgcgcgga aatgtgcgtg
     8161 aagatcaggt cgtcccaacc gtaggcagcc accagccggt agcacgcggc cagatcgacc
     8221 ctgacctgct gctccgtcac ggcgttgagg ttcggaactg ggctggtggt tgcggaatcc
     8281 atggtgcttg cctccaatgc tcgagaagat gcacaatata ccaaccggtc tacatattca
     8341 atatgatttc cgatacacag tcactgtcga gccgcgaccg catgatcgaa gcagccatcg
     8401 atctgatgcg cggctatggc ctgagcggcg tgggcatcaa cgatgtgatc cgcgagagcg
     8461 gcgcgcccag aggctcgctc tatcactttt ttccggacgg caagatccag atcgcgtcgg
     8521 aagcgctcgc agtccagggc gggcgcatct cttccttcat cgagaaggcg ttgtccagca
     8581 agaaggctgg gccccaaaag ctgctcgcac tgttcgaggc atttgcgcgg cgggtggagg
     8641 aatcggagtt tcagaaaagc tgcgcagtcg gcgccgtgac gctcgacctt gcggcggatg
     8701 ccgaaccgct tcggctggtg cttgccggca tattcgaagc gtggcgcggc gtcatcgcgc
     8761 gccatttcca gatgagcggc cccagggaag cggaatcgtt cgccgggctg gtgctgacgg
     8821 cgattgaagg cgcccacata cgcgccagag ccgaacgctc aggcgcgccg tttcgcgaag
     8881 cgggccattg gctgtcgcgg ctggtcaaac aggcgtcctg agcgaccctg ctatcccaac
     8941 tccagcgcga gcggctggtg atccgacgct tgcgtcgcgc tgtcgatctg caggccccgc
     9001 acccttcctt tgaggctttc accgacgaag acgaagtcgc aggccatcgg cttttccccg
     9061 ggcttgcggg cgtgcaggcg gaaggtgtgc ggttggggcg cccgcccgtt cagcaggcgc
     9121 caacagtccc acaaccgccc ttgggcaaaa ggccgcgtga tggcgcggta ccccggctga
     9181 tgcgattcga gattgaagtc gccgcacagg atggcgtttt cagtgtggac cttggtctgg
     9241 aacggtgagc cgtcctcgtt cgacttcggc ggcgcggcgg tgtgggcaca ggcctgcagg
     9301 tgcagctcgc gcaaggcgcg cgcctgcgcc acgcgctgag gctcggagaa gtactccagg
     9361 tgcgccgtca tcacgcgcac cgggccgagg cccgggtcga tcatcgtgac cacggtgcac
     9421 atgcggggca tgctccacat gccgccgtgc gccggatagg gcaacgggtg gtgctgtacc
     9481 tgcgccaccg ccagccgcgt cgcgaccaga ttgccgaatt ggctcctgtg gccgtccttg
     9541 ccccattcat ccaccgcggc gccgaaaaac acctgaaagc cgggcaacag ttcgcgcagc
     9601 agcgcgggtt ggtcatggcc cgcgtttccg ggcagcctcg ggtaatggac ggcgatctcc
     9661 tgcaggcaca acacatcgaa atcggccagc cgccgcgcgt ccttcacgat cctttcgggg
     9721 ttgaccacgc cgtccaggcc gcggcaccac tgggcattcc acgtgacgag tttcatggcg
     9781 gggctatccg gcgccgtggc acttcttgta tttcttgccg ctgccgcaag gacagggatc
     9841 gttgcgcccc ggctccggcg ccttgcgcac cgcctccgtg cgcgggccca ggctcttcca
     9901 taactggcgc aggtcgtaca ccgcccagat ggccgcgcca tagtcgtcca gccgctgctc
     9961 gctcacgctg ggtgggccgt cctcggcgaa catggagacg gacggcttgc cgccgtcgtc
    10021 tccggtcaat gcaacgatgg cgccgagcgc gttgtccagc atctccgcgg cctcgcgatc
    10081 gcgcggcgcg gcccagtctt cgggccaggt ttccaccgcg tacatgaagc ccagcgccca
    10141 cacctgtgcg taagagggca gggtggcgat gtcggtgtcg ccgcgctcgt cttgcggcaa
    10201 cgccagcacc gcaccgcgcg tgtccagcac ttcgggctgg taggtgcgct cgtcatccag
    10261 gctttccgcc ggcgcatcga gcgcggccgc cacctcggcc cagcggcgcc gccagcgcca
    10321 tacgaactcc atgtgcgcca ccggcttgaa gccgtcgccc agcagcacgg gccaatattc
    10381 ctcgggcgcg atcgcgcggc gcgcgcacac cagcgcggcg agaaagcctt cgcagaattc
    10441 ccactgcggg atttcctcat cgtcctcgcg cagcagatcc agctcggcgt cctgcgcgtc
    10501 gaagtcgtcg gagcccagcg gcgcgagctc gggcgcggcc ggggaatccg atgctggggg
    10561 gtcggggctg tcgttcatgc gagttcccgg ttgtggcctt gatgtgaccc ggcgatgcgc
    10621 gcgttcactt cgtcaggaag gccagcacgc ggtccgcgaa cttgccataa ggcggcatca
    10681 ggtatttcaa cgtggaaaag cgcgcctggt agaacaccgg ccgcagcttg gaaaaggtgt
    10741 ggaagccctc ctcgccatgg taatggccca tgcccgagtc gcccacgccg ccgaacggca
    10801 ggtcgtgctg gccgacatgg aacagcccat cgttgaccga cacgccgccc gacatgacgc
    10861 gctcgagcag gttttccacc gtcgagcggc tattgctgaa tgggtacagc gccagcgggc
    10921 gcgggcctgc attgatggca ttcacggcct cgtcgagcga ctggtagccg cggatcggca
    10981 ggatcgggcc gaagatctcg cgttgccaca gcgcgctctc gagcggagcg tccagcacga
    11041 gatggggcgc gatcttgcgt gtcgcgcggt cgtacgccgg accgggcacc agttggatca
    11101 gtgtggcgcc gcgctccctg gcgtcgtcca gatcggtcag cagccggtcg aacgaacgcc
    11161 ggtcgatgat cgaggtgtag tcgggcgtat ccagctgtgg gaatcgcttc ggcacgattt
    11221 cgcgcgccag ctccacgaat tcggcgatgt gcgccttggg catccacaga tggtcgaccg
    11281 tggtgcagat ctgcccggcg ttgaggtact tgacgaagag gatgcgttcc gccgccagcc
    11341 gcagcgggaa atcgtcgcac agaatggcgg gcgccttgcc accgagttcc agcgtgacgg
    11401 ggcacaggtt ctgcgccgcc gcggtcatca ccgcgcgccc cgtcgctcct gaaccggtga
    11461 acaggaggtg gtcgaagggc agcctggaga actccacgcc gacgccgccg gtctcgtcga
    11521 agaactgcag cttttcgggc ggaaagtacg ccggcatgcg ctcgatcagc agctgcgcga
    11581 gatggcgcga gttctcgctc atcttcacca tggcgcggtt gccggcggca aagatgtagg
    11641 tcaaggggat cacgctcaga ttgaggggga aattccacgg cacgatcacg ccgacaacgc
    11701 ccagcggctg cgggatcacg cggttgcgcg caccgaagaa attgcgcagg tccactttgc
    11761 gccgctgcgg cttcatccag cgccgcagct gcttgatgac gtggtcgatg ccgtcgatgg
    11821 cggggaagat ctccgccagc aaagtctcat ggcgcgagcg gtggccgtag tcggcgctga
    11881 tcgcatcgca gatcgctgtc ttgtggtcac gcacgaagcg ctgcaaggtg cgcaggtcgg
    11941 ccttgcgttc gtccagcgac ggcaccggat gggcgaggta cgcctggcgt tgccgcgcca
    12001 gcgtgcccgt gaggagttca gcgatgtcgt tcatagggct cgggcctcgt tcaggggttg
    12061 accataccca tgagcttagc cttgtcgaga agagtgccct gcctaaattc atccgtcgat
    12121 gaaccggcgg cccgaccggc cggggtttgg acgcgcgccc cgcgtgctat ggctcagcgc
    12181 gtcatttcaa gtggcgccgc cggaccgggc ccgggcgctt gatgagcgtc aaccgggtca
    12241 tccgggtcat ccggggttcg gacttttcct aggcctgctc ggcagaactg aacccgccaa
    12301 atcggacgcc gccgagcact tgattcagtg ggcgccgatg ttgaacggcc gggcgggcag
    12361 ccatagtgga agcacgcccc agggcattca cctcaacgga ttgcggagac atgaaatgat
    12421 cgattccacg cccaaccaat tcgagctgcg cttccagtcc ctgttcgaca gcgggcgcgg
    12481 ctatgcattc ccatgcgatc cgaagggtct ggtcgatctg gaccagctga gcgaccgttc
    12541 gcgcaataac tacctttacg cccgcgccat ggtcggccgc gagctggcgg tcccggcggt
    12601 gcgccccagc ggcatgcact gaggccgtcg cgcacctcga agccctaggc ggcgccgagc
    12661 aaccgcggaa tcttctgggc cgccgggccc acgtatttca ggtagcgctg aagatgcgcc
    12721 atcggctctc cgtccgtgcc tttcagcgag cggatccgcc gtgtgaacgg gtccaggcgt
    12781 gacttcgcct gccactcgcg gatgtcgggt atcttggacc aggcaagctg gttggacaat
    12841 tcggcgccca tcatgcgcac ccagtccctg ggttcgtccg gaggcgcgat cggcgcgcag
    12901 atccgatttt tctcggcctc gtcctggcag gtcgcctcga catggccgat cagcgaccag
    12961 ctgaaggtcg gctgggccaa tcgaacccac tgcagggcga tatggtcacc ggcgaaaatg
    13021 ggtttggagg gccgggcggg aatgccggtg gccgagcagt cgatgtgcag gcaatcccgg
    13081 ctggtcggaa cgacgcctcg cgcaagtacg atccgatccg attcgatccg ggtgacgcgg
    13141 ccgaggcgca ccacgtcgcg gatgcggcgc agctgcgcca gctcgccgtc gctgaccaac
    13201 ccgccgtggt aaccctcggg cttcaccgac ggatcgatcc gcctgacttc gccgaacgct
    13261 tccagccgcg cgaacacgtc gtccacggat tccccgccgg ccagggcctc caccccgttg
    13321 gcgatgctct ggatcgcgcg cgcggtgaac tcgtcgcccg gctggtaagt ggcgcggttg
    13381 atccaccagt aatcgcgcgg catgatccag cggacgcgat cgggatcggc accgttctcg
    13441 agcagccaga ggcaggcgtc cattccggtc ttgcccgcgc cgatcaccac ataggcggcg
    13501 tggggcctgg cgatgcgcgg caggtcgttc agcggcacac acacgatgtc cgggtcgacc
    13561 gggtagggcg caggccgcat cgaggggacc acggtgttcg agtaggtcgc atcgacgatc
    13621 ttgcgcgccc tgatggtgca ttgctcgccc gaaagcagcg aggtgaccgt tccatcgtcg
    13681 ccgagctcgc tcatcggaaa gtaccgcacg cgccccgtcg gcagcaaacc ctgccgcatg
    13741 acgagatcga agtaactcag tacctcggag cccgacgcaa gctcgtaaaa gcccttgttc
    13801 agcccgatcc tgtccttggc gccggatccg agcggccgcg aattgacgcc gtagaacgcc
    13861 gacggctggt gcagccgcac gaagggatag gcatggttcc agtggccacc cgggcgatgg
    13921 tgcctgtcga cgatggcgat cgtcgcagtc gaatcgctca cgaccgagtc ggcgaaggcc
    13981 attcccgcgg cgcccgcgcc cttgatcagg taatccacct cgatggctgg ttcatgcatg
    14041 tttcagctcc cgtcaggtcg tgaggccgtc gctgcgccga tacagcgcca catgataggg
    14101 ggacgtgagc tcgtcaggag ccgtttatct tcgacaatgg gcggcccaac ccatgcacac
    14161 gcgccacttc ctccaactga tcttcctttc cgcgctgtgg ggcggctcgt ttcccctcat
    14221 ccgtattgca agccccgctt tcggcccgct gggcctggcg ggcatccgct gcgtgctggc
    14281 ggccgtggtc ctggcgctgc tgatgcgcgt gctgcgagag cactggccac gcacggcgct
    14341 gacctggcgg cgcctcacgg tgctcagctt gctcacggtg gtgtccccct tcgtgctgtt
    14401 caactgggcc ggcctggtgt tgccggccgg ttactcggcc gtgctcaacg caaccgcgcc
    14461 gctgttcggc atcctgggtg ccgcggccat cggtgaggaa cgcctgacca cgcgcagatt
    14521 ggcgggctgc gccctgggct tcgcgggggt cgcgttgctg gtgcggctgg gcccggtcgc
    14581 agtcgatgcg cgggtcgtcc tggccgcgct ggcctgcacg gcggcggcgg ccagctatgg
    14641 cctgggggcg atcatgatga agcgggccac cctggcgcac cacccgctgc ccgcctcggc
    14701 cgcggtccac gttgcggctt cgttggtcct gctcctgcca acggcggcaa cggcaacgcg
    14761 gatgcaaccc accaccggcg ccttgattgc cgtggcgatc ctcggtaccg tgacatcggg
    14821 cttcatgtac tgggtcagca tgcggctgat gcgcgaaatc tccgccagcg cggcgacttc
    14881 ggcggcattc atgattccgc tgttcggcgt gacctggggc gggctgttcc tggacgagcc
    14941 ggtcacggcc gggatgctgc cgggctgtgt gctggtgctc gccgccaccg cgctcgtcac
    15001 ggggttcaat ccgttcaagg ctgtgccgcc cgagccgtga aaggcgcgcc cggcagcgcc
    15061 gtcgttggtg gaaatcgacg caatggccaa agtctccgcg tgacgggtca cggcggtgat
    15121 cgacgtcagc gcgtcacttg ccaggcgtct gcgcacgcac ctccgcaggg gggctccatg
    15181 gcgatgggtc cgggccgaca tcgtagtccg cacatccgcc cagccagagc gctgcagcca
    15241 acaccgccgc ggaaataata gtccgcgcgg tccccgccag tctgtagtcc gattccatgt
    15301 ctcctccgag ctcaacagtc ccggcattgt ggaggcgatg ttgatttgaa aacgtcacga
    15361 tctgcaaagc atggcgcgaa gtgcgtcgcg gctactcgat gccatccttc ttcagttggt
    15421 cgcgggccca tccttcgtgc gacgtgaaga agaacatctt ccatgtgctc ccttcttttt
    15481 tccatcggct gataaccggg ttgctttcga aaaccatccg gtacttgccg tcggttttgg
    15541 gaaggtacga gacggcgatc gtttttgcga ccgtggccgt accgtccggc atcatcttga
    15601 tccggaccac ttggtgggtg aaatccgtgt ccaggaaatt ggacttgaac aggtccacgg
    15661 cgttgccccg gcctttctgc tttgcgtagt aatcccaggc ctgcgtgaac cagacgtcct
    15721 ccgtcaggtg tgccgaccac gctttgacat cgtgacggtt ccaggcttgc tcgaagttcc
    15781 gcaaggcttc gcgcaattcc ttctcgactg gggcaggggc agcctgcgcg tgcaccgcac
    15841 cccctgaggc aagcgccagc acggaaacag ccaggaggag cgtgtggcgc ctgttcatga
    15901 tgcggccctt cgtcgaagtg ggggccgcca gactaatccg cggcctccgc cgaaatcgtt
    15961 gcggacagta acagtgccgc tttgggctca catttgctga tctgctcgcg cgaacatggc
    16021 ctcgaccaat cgattcggcg tgccggggtc aagcctcggt tgcgaggcta tgtagttgac
    16081 gccatacaaa cagagctagc gcggcgcgcg cgcctgcgcc acgatttcct cgctcatcgc
    16141 ctgcgcggcg atcgacaggg ctttgccctt gcgccgcgca agaaagacgg gccggaccag
    16201 gttggtgttg cgcacgggaa tggtggccag gccttcgcgc ctgaaatgga acagcgtgag
    16261 ctcgggcacc acgctcacgc ccaacccttg ggctaccagc cccgcgaccg tcgccagatg
    16321 ctccgcctcc aggccgcctg gtttgcccag cggctcggcc tgcgcggcat ccaggcagcg
    16381 ccgcacgctg ctgctgggag acagatgcac gatctcctcg ccggccagtt gggagaggct
    16441 gacagtgcgc ttgcccgcga gcgagtgggt ggcagggcac acgaggtaaa agcgttcccg
    16501 cttcagctcg atcagttcga actcacgcag gtcgccaccg gcggtcacgg ccagatcgac
    16561 ggtgccggcg cgcagcaggt ccacgctgcc ctgcgccagc gtgtcgaaca gctggacgcg
    16621 gatcatcggg ttggctgcgc ggtagcgtgc gatcacctgc ggcatccata ccgcggccac
    16681 cgaagggagg gcggcaaccg cgaccttgcc aacccggagc gtggcgaggt ctcgcaggtg
    16741 cgcaacggca aggtgcaggt c
//
LOCUS       DASWIY010000206         4520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1987885, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000206 DASWIY010000000
VERSION     DASWIY010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1987885"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="VGA59_07530"
     CDS             <1..1392
                     /locus_tag="VGA59_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9719568.1"
                     /translation="TSGTTGNPKGVVHTHRTLLDRAHAGALFDKLTHREEVLAYLPPA
                     WIGQNIFSYAQWLACGYVVNCPESSATVVIDLKEIGPTYYFAPPRVFEGLLTTVMIRM
                     EDAGWTKRNMFHTFMALARRVGPARMDGKPVGLLDRIKYTLGDIMVYGPLRNTLGFSR
                     VRVAYTAGEAIGPDLFTFYRSIGINLKQLYGSTETAVFVCLQPDNQARADTVGVPISG
                     VEIKVADNGEILVRSPGLLKEYYKNPAATAEVLTADGWYHTSDAGFLDAHGHLKIIDR
                     VKDVGRIRGGANDGAMFAPKYVENKLKFFPYIKEVVAYGDQREKACAMINIDMDAVGN
                     WAERRGLAYSGYTELAQKAEVCELIKDCVEKANADLASDPMVSGSQVHRFLILHKELD
                     PDDDELTRTRKVRRGFIAEKYAVLIDALYSGKTSQYIETQVKFEDGRKGMVAADLKIF
                     DAKTFQGDMKKAA"
     gene            1794..2597
                     /locus_tag="VGA59_07535"
     CDS             1794..2597
                     /locus_tag="VGA59_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9719569.1"
                     /translation="MRKRRKDTQIAAEGTFLRVDNVTLRFGGVKAITGVSFEIKRGEI
                     SSIIGPNGAGKSSILNVINGVYHPQEGVVTYRGKARHQMETHEAASQGIARTFQNIAL
                     FKGMTVLDNLMTGRNLQMKCNFLQQALYWGPARREELENRRKIEQIIDFLEIEDIRKT
                     PVGRLPYGLQKRVELGRALAAEPTLLLLDEPMAGMNVEEKQDMCRFILDVIDQFGTTI
                     LLIEHDMAVVMDISDFVVVLDHGEKIAEGTPDEVRSDPVVIRAYLGTAH"
     gene            2686..3639
                     /locus_tag="VGA59_07540"
     CDS             2686..3639
                     /locus_tag="VGA59_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007246480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9719570.1"
                     /translation="MQFFFEVLIGGLLSGVMYSLVALGFVLIYKASGVFNFAQGAMVF
                     FAALTFVGFTEVLPRWFGLEVGGAFVFWLALLLAFGSMILLAIGTERIVLRPLVNQPQ
                     ITLFMATIGLGFLIEGLAQGMWGAAVRGIDLGIQDEPLMWMMDRTGIGISKFDLFASG
                     VSAVLVAVLAILFNKTRTGRALRAVADDHQAALAIGIPLQQIWRIVWAAAGFVALVAG
                     LMWGARTGVQFALTFVALKALPVLIIGGFTSVPGAIIGGLIIGASEKLGEIYIPPFVQ
                     STLGLEANLSGIEGWFPYVMALLFLLVRPEGLFGEKHIDRV"
     gene            3690..>4520
                     /locus_tag="VGA59_07545"
     CDS             3690..>4520
                     /locus_tag="VGA59_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9719571.1"
                     /translation="MLYREAGQFKASYKEDQQIFPIRQDRIAIALILLFFFVAIPLVA
                     SQYWIQGILIPVLIFSLAALGLNILTGYAGQLSLGTAAFMAVGAFAAYNFMLRVPGIP
                     ILVAFILAGLCTAAVGMAFGLPSLRIKGFYLAVATLAAQFFIVWALQKFPWFSNYSSS
                     GVITAQKVEMFGYAFDSPVAKYLLTLSVVTVLALAAKNMVRSNAGRAWMAIRDMDVAA
                     EVIGIRPMHTKLMAFGVSSFYCGVAGALYAFTYLGTVEPEGFNLDLSFKILFMIIIGG
                     V"
BASE COUNT          841 a         1407 c         1407 g          865 t
ORIGIN      
        1 acctcgggaa ccacgggcaa tccaaaaggc gtcgtgcaca cccacaggac tttgctcgac
       61 cgcgcacacg ccggcgcgct gttcgacaag ctgacgcatc gcgaggaagt gctggcctac
      121 ctgcccccgg cctggatcgg acagaacatt ttcagctatg cgcagtggct ggcgtgcggc
      181 tacgtggtca attgccctga atcgtcagcg accgtcgtga tcgacctcaa ggagatcggg
      241 ccgacttatt acttcgcccc tccgcgcgtg ttcgagggct tgctgaccac ggtgatgatc
      301 cggatggaag atgcgggctg gaccaagcgc aatatgttcc acaccttcat ggcgctggcg
      361 cgccgcgtag gtcccgctcg catggacggc aagcccgttg gcctgctgga caggatcaag
      421 tacaccttgg gcgacatcat ggtctatggc ccgctgcgca acaccctggg gttctcacgg
      481 gtgcgcgtcg cctataccgc cggcgaggcc atcggccccg acctgttcac cttctatcgc
      541 tcgatcggca tcaacctcaa gcagctttac ggctcgaccg agaccgcggt gttcgtctgc
      601 ctgcagcctg acaaccaggc cagggccgac acggtcggtg tgccgatcag cggcgtcgag
      661 atcaaggtgg ccgacaacgg cgagatcctg gtgcgctcgc ccggcctgct caaggagtac
      721 tacaagaacc cggccgccac ggccgaagta ctcaccgccg acggctggta tcacaccagc
      781 gacgccggct tcctggacgc tcatggccac ctgaagatca tcgaccgcgt caaggacgtc
      841 ggccgcatcc gcggcggcgc caacgacggc gccatgttcg ctcccaagta cgtggagaac
      901 aagctcaagt tcttccccta catcaaggaa gtggtggcct atggcgacca gcgtgaaaaa
      961 gcctgcgcca tgatcaacat cgacatggac gccgtgggca attgggccga aagacgcggc
     1021 ctggcctaca gcggctacac cgagctggcg caaaaagccg aagtgtgcga gctgatcaag
     1081 gattgtgtcg agaaggccaa cgccgatctg gcatccgacc cgatggtgag cggctcgcag
     1141 gtgcatcggt tcctcatcct gcacaaggaa ctggaccctg acgacgacga actcacccgt
     1201 acgcgcaagg tgcggcgcgg tttcatcgcg gaaaagtacg cggtactgat cgacgcgctt
     1261 tattcgggca agacctcgca gtacatagag acgcaggtga agttcgagga tggccgcaag
     1321 ggcatggtgg cagccgatct gaaaatattc gatgccaaga catttcaagg cgacatgaag
     1381 aaggcggcat agacaccatg cacggaccac agttaatacg catgactgac cacactcagg
     1441 atcgttttac ctttcacgaa gcgatctcga cgctggaagt ggatttttcc catctgaccc
     1501 tgagcaacgc ggcgcaggtc gatgcctttt acgacgccat cgacgatcgc ctggccgcga
     1561 gcggcaaacg ctggttcttc ctggtggact acaccgactg cgtgatcgcg ccggaagtct
     1621 ggcagcgctt cgcggaacgc ggcaagaaca gcaacgtgac ctacggtctg ggcaccgtgc
     1681 gcgtcggcgc gacctcacag acgcgcgagg cgatccgcgc cgatgccacg cgcgccatgt
     1741 tccgcgccaa tatctacgac acgcgcgatc aggcgatgct ggcgctgggc gaaatgcgca
     1801 agcgccgcaa ggacacgcag atcgcggcgg aaggaacctt cctgcgcgtc gacaacgtca
     1861 ccttgcgctt cggcggcgtc aaggcgatca ccggggtcag cttcgagatc aagcgcggcg
     1921 agatctcgtc catcatcggg cccaacggcg ccggcaagag ctcgatactg aacgtgatca
     1981 acggcgtgta tcacccgcag gaaggcgtcg tcacctaccg cggcaaggcg cgccaccaga
     2041 tggagacgca cgaagcggcc tcgcagggaa tcgcccgcac tttccagaac atcgcgctgt
     2101 tcaagggcat gaccgtgctc gataacctga tgacggggcg caacctccag atgaagtgca
     2161 acttcctgca gcaggcgctg tattgggggc cggcgcggcg cgaagaactt gaaaatcgca
     2221 ggaagatcga gcagatcatc gacttccttg agattgagga tatccgcaag acgcccgtgg
     2281 gacggctgcc ctacggcctg caaaagcgcg tcgagctggg acgcgcgctg gccgcggaac
     2341 cgacactgct gctgctcgac gaaccgatgg ccgggatgaa cgtggaggaa aagcaggaca
     2401 tgtgccgctt catcctggat gtcatcgacc agttcggcac aaccattctt ctcatcgagc
     2461 acgacatggc ggtggtcatg gacatctccg atttcgtggt cgtgctggac cacggcgaaa
     2521 agatcgccga aggcacgccc gatgaggtcc gttcggatcc ggttgtgatc cgcgcctatt
     2581 tgggaacagc gcactgaacg caggtgtggt tcggccttcg ggccgggcag gcaggtggtt
     2641 ggggaacgtc gggcttcaag cccggcctga cggagcaatg aggatatgca attctttttc
     2701 gaagttctga tcggcggtct gctctccgga gtcatgtatt ccctggtcgc actgggtttc
     2761 gtcctgatct ataaggcctc gggcgtgttc aacttcgccc agggcgcgat ggtttttttc
     2821 gcggcgctga ctttcgtcgg attcaccgaa gtgttgccgc gctggtttgg cctggaggtg
     2881 ggcggggcgt ttgttttctg gcttgcactg ctgctggcgt tcggctcgat gatccttctc
     2941 gccatcggaa ccgagcgcat cgtcctgcgg ccgctggtaa accagccgca gatcacgctg
     3001 ttcatggcca cgatcgggct gggtttcctg atcgagggat tggcccaggg catgtggggc
     3061 gccgcggtgc gcggcatcga cctcggcatc caggatgaac cgctcatgtg gatgatggac
     3121 aggacgggga taggcatctc caagttcgat ctgttcgctt cgggcgtctc ggccgtcctg
     3181 gttgccgtgt tggccatcct gttcaacaag acgcgcaccg gccgcgcgct gcgcgccgtg
     3241 gccgacgacc accaggcggc gctcgccatc ggcatcccgc tgcagcagat ctggcgaatc
     3301 gtgtgggccg cggccggctt cgtcgcgctg gtggcgggcc tcatgtgggg cgcgcgcacc
     3361 ggggtgcagt ttgccctgac cttcgtcgca ctgaaggcgc tgccggtgct catcatcggc
     3421 ggtttcacct cggtgccggg ggcgatcatc ggcggcctca tcatcggtgc cagcgagaag
     3481 ctgggcgaga tctacattcc cccgttcgtg cagagcacgc tcgggctgga ggccaacctg
     3541 agcggcatcg agggctggtt cccgtacgtg atggcgctgc tcttcctgct ggtcaggccg
     3601 gaggggctct tcggcgagaa acacatcgat cgcgtttagt ccggcgcaac ccacggccca
     3661 accctccaac tcgaatcccg gaacccatca tgctctatcg cgaggcaggc caattcaagg
     3721 ccagttacaa ggaagaccag cagattttcc cgatccggca agaccgcatc gcaatcgcgc
     3781 tgatcctgct gtttttcttt gtggcgattc ccctggtcgc cagccagtac tggattcagg
     3841 gcattctgat cccggtgctg atcttttcgc tggcggcact cgggctgaac atcctcaccg
     3901 gttatgccgg ccagctctcg ctcggtacgg ccgccttcat ggcggtgggt gccttcgccg
     3961 cctacaactt catgctgcgc gtgccgggca taccgatcct ggtcgcgttc atcctggccg
     4021 gcctttgcac cgccgcggtc ggcatggcat tcggactgcc gtcattgcgc atcaagggct
     4081 tctacctggc ggtagcgacc ctggcggcgc agttcttcat cgtctgggcg ctgcagaagt
     4141 tcccctggtt ctccaactac agttcctcgg gcgtgatcac ggcgcaaaaa gtcgagatgt
     4201 tcggctatgc gttcgactcg cccgtcgcca aatacctgct gacgctcagc gtcgtcaccg
     4261 tgcttgccct ggcggccaag aacatggtgc gcagcaacgc cggccgcgcc tggatggcca
     4321 ttcgcgacat ggacgtggca gccgaggtga tcggcattcg tccgatgcac acgaaactga
     4381 tggccttcgg cgtcagttcg ttctactgcg gcgtcgccgg cgcgctatac gctttcactt
     4441 acctgggcac cgtggagccc gaaggcttca acctcgacct gtcgttcaag atcctgttca
     4501 tgatcatcat cggtggcgtg
//
LOCUS       DASWIY010000207         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_116512, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000207 DASWIY010000000
VERSION     DASWIY010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116512"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1153
                     /locus_tag="VGA59_07550"
     CDS             281..1153
                     /locus_tag="VGA59_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9719572.1"
                     /translation="MSAYDQKSAMLFLAPMVAVLMVVAVFPVVYSFWISLFDLKLTRP
                     HRAPFVWFDNYVKIFQDSLFWESVGRTTSFTVMSVAAIMVIATLMALFLNEEFRGRRF
                     LSAILLIPWAIPYVANGLMWKWIYDSGYGALNGLLLQLGLIDEYLVWLGDVDKTLVLI
                     TNSFVWKEVPLATILVLVTLKSIRADLYSAAKVDGANTWQRFVHITLPQLVPGFALVM
                     IYETMMAIRHFDLFFILTEGGPAEASHVVAWQVYVETFRKLAFGTGAALAYIMAIATF
                     TLAFFIIRSTSRKL"
     gene            1164..2081
                     /locus_tag="VGA59_07555"
     CDS             1164..2081
                     /locus_tag="VGA59_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9719573.1"
                     /translation="MTFMKIPHLGRRALLFAASLLFSFYVLAPVAWLVSSSFQSEAEI
                     TSVPPHWLPHQPTAENFAAIFKAKETVVTYENRRQGDSATGGFIPSTAKNLLPGMWNS
                     LVVALAVVVLNLLVSVPAAYALAKIRFIGRTSSIYFMLTSRVIPDIALVVPFFLFVQK
                     LGLLDNLLSLVITYLAITVPFSIFILTGYFESLPDELDKAARVDGCSRFQTMTLVFLP
                     LALPSLVAVVLFTFLTSWNEFLLALMFTQTPASQTMPIVVASFTSDFTISFSFINAAG
                     VLAIVPPVIIAILFERYIVSGLTAGAVKG"
     gene            2086..3186
                     /gene="ugpC"
                     /locus_tag="VGA59_07560"
     CDS             2086..3186
                     /gene="ugpC"
                     /locus_tag="VGA59_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX9719574.1"
                     /translation="MASITFESVGKKYPNGFVALESLNLDIADKEFLVLLGPSGCGKS
                     TTLNMIAGLEDVTDGNLFFDKDVMNTVPPHKRDVAMVFQSYALYPHKTVYDNIAFGLR
                     MRKHRKDEIDRRVRDAARRLEIEPLLERRPDQLSGGQRQRVALGRAMVRQPAVFLMDE
                     PLSNLDAALRISMRAEIKQLHQVMQTTFVYVTHDQAEALTLADRIVVMRGGVVQQIGT
                     PDAIYEQPRNMFVASFLGNPPINFLEGRLALDAGRIVFQRGQLSLTLPAHVAERVGGQ
                     LGREVVLGIRAEDVSQVPSSAVSEGVQGRIISVLPVGSDQFVEMEVEGTRLFFRLGKE
                     VRHKDGDSATLAVNLNRLHLFDKQTTQSLVWH"
     gene            3336..>3476
                     /locus_tag="VGA59_07565"
     CDS             3336..>3476
                     /locus_tag="VGA59_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9719575.1"
                     /translation="MKPAAFAYLRPASIEEALEQLAHFGEEAKVVAGGQSLGPMMNMR
                     LAT"
BASE COUNT          558 a         1129 c         1111 g          678 t
ORIGIN      
        1 cctttccgta gctattctcc ttcgaggaga tggccaaagg gcactgccat tgagctacct
       61 gattaacgtc aatcttccgc agcccccata agctttccct ctccctccgg gagagggtcg
      121 gggtgaggga gccggcgctg atgtgatccc aaggcgccgt aaggcttttc tcggggtttt
      181 gagctgtttg ccggattcat cgctatggcg ctgccctcac cctaaccctc tcccgaaggg
      241 agaggggacg agacgagctc gccggtggct cggccatccc gtgagcgcgt acgaccagaa
      301 aagcgccatg ctgttcctgg cgcccatggt cgccgtgctc atggtggtcg ccgtgttccc
      361 ggtcgtctat tcgttctgga tcagcctgtt cgacctgaag ctgacgcgcc cgcaccgcgc
      421 gccgttcgtc tggttcgaca actacgtgaa gatcttccag gactcgctgt tctgggagtc
      481 ggtgggcagg acgactagct ttaccgtgat gtcggtggcc gccattatgg tgatcgcgac
      541 gctcatggcc ttgttcctga acgaggaatt ccgcggccgc cgcttcctgt cggccatact
      601 gctgattccc tgggccatcc cctatgtcgc caacggcctg atgtggaagt ggatctacga
      661 ctcgggctat ggcgcgctca acgggctgct gctgcagctg ggcctgatcg acgagtacct
      721 ggtgtggctc ggagacgtgg acaagacgct ggtgctgatc accaactcgt tcgtctggaa
      781 ggaagtgccg ctggccacca tcctggtgct ggtcacgctc aagtcgatcc gtgccgatct
      841 ttacagcgcc gccaaggtcg acggcgcgaa cacctggcag cggttcgtgc acatcacgct
      901 gccacagctg gtgcccgggt tcgcgctggt catgatctac gaaacgatga tggcgatccg
      961 ccacttcgac ctgttcttca tcctcaccga gggcgggccg gcggaagcgt cgcatgtcgt
     1021 cgcctggcag gtgtacgtgg agactttccg caagctggcc ttcgggaccg gcgcggccct
     1081 ggcctacatc atggcgatcg ccaccttcac gctggcgttc ttcatcatcc gcagcacgag
     1141 ccgcaagctg tagggacgct gcgatgacgt tcatgaagat tccccacctc ggacggcgcg
     1201 cgctgctgtt cgccgcgtcc ctgctgttct cgttctacgt gctcgcgccc gtggcctggc
     1261 tggtgtcgtc atcgttccag tcggaggcgg aaatcacctc cgtgcctcct cactggcttc
     1321 cgcaccagcc gacggcggag aatttcgcgg ccatcttcaa ggccaaggag acggtggtca
     1381 cctacgagaa tcgcaggcag ggcgacagcg ccaccggcgg gttcatcccg tccacggcca
     1441 agaacttgct gcccggcatg tggaacagcc tggtcgtcgc gctggcggtt gtggtgctga
     1501 acctgctggt gagcgtgccg gcggcctacg cgctggccaa gatccgcttc atcgggcgta
     1561 cctcgtcgat ctatttcatg ctgacctcgc gtgtcatccc ggacatcgcg ctggtggtgc
     1621 cgtttttcct gttcgtgcag aagctggggc tgctggacaa cctgctgtcg ctcgtcatca
     1681 cctacctggc gatcacggtg ccgttcagca tcttcatcct gaccggctat ttcgagtcgc
     1741 tgccggacga gctggacaag gcggcgcggg tggatggctg ttcgcgcttc cagacgatga
     1801 cgctggtgtt cctgccgctg gcgctgccgt cgctggtggc ggtggtgctg ttcaccttcc
     1861 tcaccagctg gaacgagttc ctgctggcgc tgatgttcac gcagacgccc gcgtcgcaga
     1921 ccatgcctat cgttgtggcg tcgttcacgt ccgacttcac catcagcttt tcgttcatca
     1981 acgccgccgg cgtgctggcc atcgttccgc cggtgatcat cgccatcctg ttcgagcgct
     2041 acatcgtctc cggcctcacg gccggcgccg tcaaggggta agcaagtggc aagcatcaca
     2101 ttcgagtccg tgggcaagaa gtaccccaat ggcttcgtcg cgctggagag cctgaacctc
     2161 gacatcgcgg acaaggagtt cctggtcctg ctcggcccct cgggctgcgg caagtccacc
     2221 acgctgaaca tgatcgccgg gctggaggac gtgaccgacg gcaacctgtt cttcgacaag
     2281 gacgtgatga acaccgtgcc gccacacaag cgcgacgtgg cgatggtgtt ccagagctac
     2341 gcgctgtatc cgcacaagac ggtgtacgac aacatcgcct ttggcctgcg catgcgcaag
     2401 caccgcaagg acgaaatcga tcggcgggtg cgggacgccg cgcgccgcct ggagatcgag
     2461 ccgctgctgg aacgccggcc cgaccagctc tcgggcggcc agcgccagcg ggtggcgctc
     2521 gggcgcgcca tggtgcgcca gcccgccgtg ttcctcatgg acgagccgct gtccaacctg
     2581 gacgcggcgc tgcggatctc gatgcgcgcg gagatcaagc agttgcacca ggtgatgcag
     2641 accacgttcg tttacgtcac ccacgaccag gccgaggcgc tgacgctggc cgaccgcatc
     2701 gtcgtgatgc gcggcggcgt cgtccagcag atcggcacgc cggacgccat ttacgagcag
     2761 ccgcgcaaca tgttcgtggc ttcgttcctg ggcaatccgc cgatcaactt cctggagggc
     2821 aggctggccc tggacgccgg gcgcatcgtc ttccagcgcg gtcagttgag cctgaccttg
     2881 ccggcccatg tggccgaacg tgtgggcggg caactggggc gcgaagtggt cctgggcatc
     2941 cgcgccgagg acgtttcgca ggtgccgtcc agcgccgtct ccgagggtgt gcagggccgc
     3001 atcatctcgg tgctgccggt gggctccgat cagttcgtcg agatggaggt cgaaggcacg
     3061 cggctgttct tccgcctggg caaggaagtg cgccacaagg acggcgacag cgccacgctc
     3121 gccgtgaacc tcaaccggct gcacctgttc gacaagcaga ccacccagag cctggtgtgg
     3181 cactgatgtg aaagcccacc cccgaagcgc cttcggcgcc tccccctcaa gggggcgcca
     3241 ccagcggccc ggcaaagccg gttccgcggt ggcccccgca ctgatcgcac gatttcgtac
     3301 gttgcgggtg acacgaagcg caatggatca ctgacgtgaa gcccgccgca ttcgcctact
     3361 tacggcccgc ctcgatcgag gaggcgctgg agcagctcgc gcacttcggc gaggaggcga
     3421 aggtcgttgc gggcgggcag agcctggggc cgatgatgaa catgcggctg gccaca
//
LOCUS       DASWIY010000208         2872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3094863, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000208 DASWIY010000000
VERSION     DASWIY010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3094863"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..481)
                     /locus_tag="VGA59_07570"
     CDS             complement(83..481)
                     /locus_tag="VGA59_07570"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX9719576.1"
                     /translation="MTASKALSRRRAWVPDRQAVIWIDCNTQVGREMRDVHPFLVLSP
                     RIFNEKTSLVIGLPMTPAAYNADNPFAVAVGKASGRKAGQIGYVLCHQPKSFDWRLRG
                     AKAHPLGTLPDTLFGQACDRLNQIIQLEHA"
     gene            complement(478..771)
                     /locus_tag="VGA59_07575"
     CDS             complement(478..771)
                     /locus_tag="VGA59_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9719577.1"
                     /translation="MLTLTFRHLERQAMAEAVLDIKQWGNSLGVRLPAAVAREAHLRV
                     DQRVRVSVEGARVVITPLAPASPILEQRLANFDPARHGGEAMATGPALGAERW"
     gene            810..1892
                     /locus_tag="VGA59_07580"
     CDS             810..1892
                     /locus_tag="VGA59_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HEX9719578.1"
                     /translation="MSATIDRRKLLLAGLGAAAGAGAKDAGSPADALIAKALQFPDHH
                     GAHPDLRTEWWYLTGHARSGVREFGFQVTFFRSRVEATQGMTSRFAAKQLVFAHAAVT
                     DVQGRKLWHDQRIAREGFEVALAAQGDTRVKLRDWSLERQPDFYRARMAAGDFTLDLQ
                     FSPTQPVLLQGQQGLSRKGPLVENASYYYSQPQLAVRGSVALQGQRFDVTGKAWLDHE
                     WSNEYMHQDAVGWDWLGMNLDDGSALTAFRLRKQDGTALWDGGSFRSPEAGLYTFSNG
                     EVVFSPQRRWTSPLSQATYPVEWVVRTPADIYTVKAVIDNQELDSRASTGAIYWEGLS
                     DLLDSNGKRVGRGYLEMTGYAQRLRM"
     gene            2054..>2872
                     /locus_tag="VGA59_07585"
     CDS             2054..>2872
                     /locus_tag="VGA59_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX9719579.1"
                     /translation="MTRAMQIAPAHLNQARAVASILLLVGGAALADGVTIGGRLDVGL
                     QSIDDGTSKTTRVDSGTYGFSRLFFKGVEDLGGGLGALFYLEHRFNADTGLQINPVKY
                     WNGGSYVGLSSTRWGTLTLGRQFPPIFYTIVSADESGPGHGHGYTAMASMQRTDLFRI
                     AAAASPVKVAGSFDAIAGGIHSVAFSSGLEDNLVAYASPTFSGAAVRLAAGLPEGYLP
                     GNAKIFGGNVEYRNDRFFGSVALNSKEGRVPAGGAATQKLREAVVSGMYGLSSAS"
BASE COUNT          507 a          899 c          959 g          507 t
ORIGIN      
        1 gtgctggcgg tgaaggagga ttggtgaacc cggccgcacg gatggcaggc gccggcatca
       61 gcgagacccg cacttgggca ggttacgcgt gttccaactg gatgatttga ttcagccgat
      121 cgcatgcctg cccgaagagg gtatcgggca atgtccccaa cggatgggct ttggcgccgc
      181 gcaagcgcca atcgaacgac ttgggctgat ggcaaagaac atagccgatc tgtcccgcct
      241 tgcgacccga agctttgcct acggccacgg caaaggggtt gtcggcgttg taggccgccg
      301 gggtcatggg caatccgatc accagggacg tcttctcgtt gaagatgcgc ggcgacagca
      361 ccaggaaggg atgcacatcg cgcatctcgc gcccgacctg ggtgttgcag tcgatccaga
      421 tgacggcctg gcgatccggc acccaggcac gccggcgtga cagtgccttt gacgccgtca
      481 ccaacgctca gccccgagcg cgggcccagt ggccatggcc tcgcctccgt gccgcgccgg
      541 atcgaagttg gccaaacgct gttccaggat gggggacgcc ggcgcgagcg gcgtaatgac
      601 cacccgcgca ccctcgaccg agacgcgaac gcgctggtcg acgcgcaggt gcgcctcgcg
      661 tgccacggcc gccggcaggc gcacgcccag gctgttgccc cattgtttga tgtcgagtac
      721 cgcctcggcc atggcctgtc tttccaaatg tctaaacgtg agtgtcaaca tgcgcttgtc
      781 tccagtcaag gcggtgcgaa aatctcccca tgagcgcaac cattgaccgc cgaaaacttt
      841 tgctggcagg gctaggggct gcggctggag ccggcgcgaa agatgccggc agtcctgcgg
      901 acgctttgat cgccaaggct ctgcagtttc cggaccacca tggcgcccat ccggatctca
      961 ggaccgagtg gtggtacctc accggccatg cgcgatccgg cgtgcgcgag ttcggcttcc
     1021 aggtgacgtt cttccgctcg cgcgtggagg cgacgcaggg catgacctcg cgcttcgcgg
     1081 ccaagcagct ggtgttcgcg catgccgcgg ttacggatgt gcaaggccgc aagctctggc
     1141 acgaccagcg catcgcgcgc gaaggcttcg aagtcgcgct cgccgcgcag ggggacacgc
     1201 gagtcaagct gcgcgattgg tcgctggagc ggcagcctga tttctatcgc gcgcgcatgg
     1261 cggccggcga tttcacgctc gacctgcaat tcagcccgac gcagccggtg ctgctgcaag
     1321 ggcagcaggg cctgtcgcgc aaaggcccac tggtggaaaa cgccagctac tactacagcc
     1381 aaccgcagct cgcggttcgg ggcagcgtcg ccttgcaggg acagcgcttc gacgtgacgg
     1441 gcaaggcttg gctcgaccac gaatggagca atgaatacat gcaccaagat gcggtcggct
     1501 gggactggtt gggcatgaac ctggacgatg gcagcgcgct caccgcgttt cgcctgcgca
     1561 aacaggacgg cacggcgctg tgggacggcg gctcgttccg ctcgcccgag gcgggcctgt
     1621 acaccttcag caatggcgaa gtcgtcttca gtccgcagcg gcgctggacc agcccgctgt
     1681 cgcaggccac ctaccccgtc gagtgggtcg tgcgcacccc cgccgacatc tacaccgtga
     1741 aggccgtgat cgacaaccag gagctggaca gccgtgcgtc caccggcgcg atctactggg
     1801 agggcctgtc agacctgttg gacagcaacg gcaagcgggt gggccgcggc tacctggaga
     1861 tgacgggcta tgcgcagcgg ctgcggatgt aggccagtcc gttccgatgg gcggctgagc
     1921 ttggcgtaat cgccgacatc gaacccgcta agaccgagtt cacaccccgt tttccggcat
     1981 tcgtcgcata tgcccttgtt tcggctgccg atccctgcca gagtgcaatc tcaacgacgc
     2041 tcaggggaaa gccatgactc gcgcgatgca gattgcaccg gcccacctca atcaagctcg
     2101 cgccgtcgct tcgatcctgc tgctggtcgg tggcgctgcg ctcgcggatg gcgtgacgat
     2161 cggggggcgc ctggatgtcg ggctgcagtc gatcgacgac ggcacgtcga aaacgacacg
     2221 cgtggacagc ggaacgtatg gcttttctcg cctgtttttc aagggagtcg aggacctggg
     2281 cggcggcttg ggcgcgcttt tctatctgga gcatcgtttc aatgcggaca ccggcttgca
     2341 gatcaatccc gtcaagtact ggaatggggg cagctacgtc ggcttgtcga gcacgcggtg
     2401 ggggactctc acgcttggtc gccagttccc acccattttc tatacgattg tgtccgccga
     2461 cgagtccggg cccggccacg ggcatggcta taccgctatg gcatccatgc agcgaacgga
     2521 cttgttccgc atcgccgcgg cggcttcgcc ggtcaaggtg gcaggctcgt tcgacgcgat
     2581 cgctggtgga atccactcgg tcgcgttcag cagcggcctg gaagacaacc tggtcgcata
     2641 cgcctcgccc acattcagcg gcgcggcagt gaggctggca gcaggattgc ctgaaggcta
     2701 tctccccggc aacgcgaaga tattcggcgg caatgtggaa taccgcaatg atcgtttttt
     2761 cggatcggtc gcgttgaact cgaaggaggg cagagtgccg gccggcggcg cggcaaccca
     2821 gaagctgcgg gaagcggtcg tatcggggat gtacgggctc agcagcgcgt cg
//
LOCUS       DASWIY010000209        12668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_857457, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000209 DASWIY010000000
VERSION     DASWIY010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 12668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12668
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857457"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /gene="uppS"
                     /locus_tag="VGA59_07590"
     CDS             <1..605
                     /gene="uppS"
                     /locus_tag="VGA59_07590"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX9719580.1"
                     /translation="CVRHCGERGVRVLTVFAFSSENWNRPAEEVSSLMELLAMALARE
                     VPQLKTEGVRLHFVGDRVGLADKVRAGLAQAEADTAGNDRLILNVCFNYGGRWDIAQA
                     AARLAARGEPITEQSMGTALALAHVPDPDLVIRTGGERRLSNFLLWQAGYAELYFSDK
                     LWPEFDAAALDEALSDYATRERRFGKTPEQLSTHDAKKVA"
     gene            623..1477
                     /locus_tag="VGA59_07595"
     CDS             623..1477
                     /locus_tag="VGA59_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HEX9719581.1"
                     /translation="MLKQRIITAVVLLAILLPALFWPSPVPFMAVALILIGAGAWEWG
                     RLNGLAQTGALLLAGVCVALCAASWNTVLLPGPLPFVWFFAGVVWVMGGAWLLRVGVA
                     GWPRIPRMVRLAAGVLALWLAWLAVAKARVVGINFLLSVLVLVWVADIAAYAAGRTFG
                     LKFTRNRLAPSISPGKSWEGVWGGMAGVLVLAVAWVLADRALQAGVPSLYTRLADAGW
                     WLLVIGAVFLAAMSVVGDLVESLIKRSAGVKDSSNLLPGHGGVLDRVDALLPALPLAM
                     MLTTLAYR"
     gene            1474..2664
                     /gene="ispC"
                     /locus_tag="VGA59_07600"
     CDS             1474..2664
                     /gene="ispC"
                     /locus_tag="VGA59_07600"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX9719582.1"
                     /translation="MKQRITVLGSTGSVGANTLDVIARHPGRFEVFALGAATQVDLML
                     RQCARFKPAFAVMGSEAAAAELARKIEAGGLATRVRWGQAALEEMASHELVDTVMAAI
                     VGAAGLASCLAAARAGKRLLLANKEALVVGGELFLAAMKQGGAMLLPIDSEHSAIFQS
                     LPEDAASWEARVEKIILTASGGPFRTREPATLRNVTPEEACAHPNWVMGRKISVDSAT
                     MMNKALEVIEARFLFGLAPERLEVVIHPQSVIHSMVQYRDTSVVAQLGTPDMRVPIAY
                     GLAWPDRVVSGAQALDFHTLADMSFESLDSRGHRERFPGLALAWETLRAPPGTTAVLN
                     AANEEAVAAFLDRRIRFDQIHEVNVATLEAVTASNPESLEDLLDLDARSRSAAARAIA
                     RLLS"
     gene            2679..4052
                     /gene="rseP"
                     /locus_tag="VGA59_07605"
     CDS             2679..4052
                     /gene="rseP"
                     /locus_tag="VGA59_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX9719583.1"
                     /translation="MLTIVAFIVALSLLVAVHEYGHYRVAVACGVKVLRFSVGFGKIL
                     YRWQPKRRKPGQSTEFVIGLFPLGGYIKMLDEREGPVAPEERHLAFNTQPLKSRTAIV
                     AAGPIANLLLAVVLFATVNWYGVEEPKPVLASPAPGSLAERAGLRGGEEVEQAGFDGE
                     ALHSLRSFGELRWLLTRGAVDRRDVRLQLARDRQGARGQALLALSQVQSADVDASLSR
                     KIGIMGPWTPPAIGEVMAGGAAERAGLRKGDLVLSIGQTRIVDGQQLREMIRDSAKAG
                     KPGAEKWQVRRGGQTLELPVVPDAVQQGETVIGRIGAYVGAAPELVTVRYGPLEGLWK
                     GVERTWEVSVLTLRMMGRMVIGEASLKNLSGPLTIADYAGKSASMGLTQYLVFLALIS
                     VSLGVLNLLPLPVLDGGHLMYYLWEAVTGRGVSDAWMERLQRGGVAVLLVMMSIALFN
                     DVARLFG"
     gene            4107..6401
                     /gene="bamA"
                     /locus_tag="VGA59_07610"
     CDS             4107..6401
                     /gene="bamA"
                     /locus_tag="VGA59_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX9719584.1"
                     /translation="MKKQIKRFRVRAVSAMVAVAFVANAWAVDPFTVRDIRVEGLQRV
                     EPGTIFASLPFRIGDQYNDEKGSAAIRALFALGLFKDVRLEVSGDVLVVIVEERPTVA
                     DVDFVGAKEFDKDALKKALREIGLTEGRPYDQGLADRAEQELKRQYINKSLYGAEVVT
                     TVTPIERNRVNLTFTVTEGEPARIKEIRVVGNRAFSESTLKGLFDLDTGGFLSWYTKS
                     DRYSRAKLNADLEALRSYYLSRGYLEFRIDSTQVALSPNKQDITITVNVTEGERYVVS
                     GVKLEGNYLGKEEEFKSLVTIRPGQPFNADQVAETTKAFTDYFGNFGYAFATVEARPE
                     IDRANNRVAFVLLAEPSRRAYVRRVNIAGNNRTRDEVIRREFRQFEASWYDADKIKLS
                     RDRIDRLGFFREVNIETSDVPGAPDQVDLNITVVEKPTGSLQLGAGFSSAEKLALSFS
                     IKQENAFGTGNYLGVEVNTSKYNRTVAVTSVNPYFTADGISRTVDLYHRTSRPYEDQG
                     GNYQLATTGAGLRFGVPFSELDTVYFGGSVERTEIRPGTNIPAAYLAYADRFGFTSSS
                     IPLSIGWSRDSRDSAIAPNSGRYQRVAGEVGVGGDARYLRANYQFQQYVPLNKQYTLA
                     FNGEAGWGKGLGGRPYPVFKNFYSGGLGSVRGFDQGTLGPRDVTGSSIGGPKKITLNA
                     EVITPFPGAGNDRTLRLFGFVDVGNVFGEHEKIHFGDMRASVGVGLSWISPIGPLRLA
                     VAQPIRKFAGDRIQRLQFQIGTSF"
     gene            6401..6910
                     /locus_tag="VGA59_07615"
     CDS             6401..6910
                     /locus_tag="VGA59_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX9719585.1"
                     /translation="MKYLSRQWLVIFLGVLALAAGAHAQDFKVGFVNTDRIFREANTA
                     KAAQAKLEQEFSKREKELNDLGNGLKMLSDKFEREAPTLSESQRAQRQKQILDQDRDF
                     QRKRREFQEDLNARKNEELQQVLERANRVVKQVAEAEKYDVVLQEAVYINPRHDITDK
                     VIKALNASR"
     gene            6910..7887
                     /gene="lpxD"
                     /locus_tag="VGA59_07620"
     CDS             6910..7887
                     /gene="lpxD"
                     /locus_tag="VGA59_07620"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX9719586.1"
                     /translation="MQLRLGSIVETLGGELCGNPERLIEGLAPLESANPAQLSFLSNP
                     KLAQQLATSRAACVIVAPALREAALARGDCIVAEQPYLYFARVTQLWKKRLGAAEGPL
                     IHSTAVIDREAVVDPSARIGPLCVIARGARIGAGTVLKARVSVGEACVIGDRCIIHPG
                     AVIGADGFGFAPDGTGGWEKIEQLGAVRIGNDVEIGANTCIDRGALQDTVIGDGVKLD
                     NLIQVGHNVRIGRHTAIAGCAGIAGSATIGAYCTIGGAAMILGHLRLADHVNISAGTF
                     VGRSILKPGHYTGIFPMDDNAQWEKNAATLKQLHKLRDRVKALEKKTEP"
     gene            7884..8324
                     /gene="fabZ"
                     /locus_tag="VGA59_07625"
     CDS             7884..8324
                     /gene="fabZ"
                     /locus_tag="VGA59_07625"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX9719587.1"
                     /translation="MMDIHQILKHLPHRYPFLMVDRVLEIDKGKSIRTLKNVTINEPY
                     FAGHFPHRPVMPGVLMLEAMAQSAALLSFATMGVMPDDKTVYYFAGIDAARFKRPVEP
                     GDQLLMDVTLIRSKAGIYKYKGIARVGEELACEAELMCTMRTVG"
     gene            8339..9133
                     /gene="lpxA"
                     /locus_tag="VGA59_07630"
     CDS             8339..9133
                     /gene="lpxA"
                     /locus_tag="VGA59_07630"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX9719588.1"
                     /translation="MTRIHPTAIVDPGAELDATVSVGPYTVIGAHVRIAGGTTVGPHC
                     VIEGRTTIGRDNRIFQFASLGAIPQDKKYAGEPCKLVIGERNTIREFSTYNIGSPGGG
                     GITRLGNDNWMMAYVHLAHDCIVGDHTIFANNAQLAGHVEVGDWAILGGFTTVHQFVR
                     IGAHGMTSLNAMLVADQPPFVMSQGQPAQARSMNFEGLRRRGFAAERIAAVKAMHKAL
                     YRDGLTLEQSKQRIAALAEKTPQAAPDVQMMLSFLDRVSPQRGIVR"
     gene            9148..10266
                     /gene="lpxB"
                     /locus_tag="VGA59_07635"
     CDS             9148..10266
                     /gene="lpxB"
                     /locus_tag="VGA59_07635"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX9719589.1"
                     /translation="MVAGEASGDLLAGLLLDGLRRRWPGLQAAGIGGPQMARRGFEAW
                     WPHEKLAVSGYVEVLRHYREIVGIREQLRRRLLEHPPQLFIGIDAPDFNLDLEAALKS
                     RGIRIVHFVCPSIWAWRPQRAAKLKASADHVLCVFPFEPELLARHGIESTYVGHPLAS
                     LIPMKPDKAAARRALGLEAAGEVLAILPGSRASEVHRLAGRFLAAAALVLRKRPGMKL
                     VVPALPARREAVEEAARAAGLGDGLQIVQGQSHTVLAACDLALVASGTATLEAALFKR
                     PMVIAYAVNWLSYRMMKPRHLQPWIGLPNILCGEFVVPELTQHDANPESLAAALLDWL
                     EAPARMAAVQEKFTALHALLQRDTATLATDAIEKVIQG"
     gene            10358..11011
                     /gene="rnhB"
                     /locus_tag="VGA59_07640"
     CDS             10358..11011
                     /gene="rnhB"
                     /locus_tag="VGA59_07640"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX9719590.1"
                     /translation="MAGVDEAGRGPLAGPVVAAAVILDDTKRIRGLADSKVLTPLQRD
                     RLYDQIREKALCCSVAQASVEEIDTLNILWATMLAMKRAVEGLRLKPAKVLVDGNRLP
                     VVDMLAEAVIGGDATVKAISAASILAKVHRDRLCQALHAEFPQYGFASHKGYATPEHL
                     EALRVHGACHHHRRFFGPVAATFALGDGAVADFALRDEAAAAFAIEEVGVVVAGIVA"
     gene            11008..11787
                     /locus_tag="VGA59_07645"
     CDS             11008..11787
                     /locus_tag="VGA59_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9719591.1"
                     /translation="MTSSDPQFVSSAANALLKDLRRLAQDSSAYRKQGRVWLEGDHLC
                     RAAMARGVQPALAVFSESSWSAGESAWAGRAGKHVVIADALFAGLSGLESPARMGFVV
                     DLPPAPALRPDVCTVILDRVQDAGNVGAILRCAAAFGFGQVLAIKGTAALWSPKVLRS
                     GMGAHFGLRLIESLAPEALDALALPIITTSSHQGELLHRRELPWPCAWALGHEGQGVS
                     PEIAARSRLAVRIVQPGGEESLNVASAAAICLHASAAAHAG"
     gene            11968..>12668
                     /locus_tag="VGA59_07650"
     CDS             11968..>12668
                     /locus_tag="VGA59_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HEX9719592.1"
                     /translation="MLLPHQGTFPPAILALADGTVFLGNSIGATGSTTGEVVFNTAMT
                     GYQEILTDPSYCQQIVTLTYPHIGNYGVNEEDVEACKIHAAGLIIRDLPLVASNFRTS
                     QSLSQYLRSQNTVAIANIDTRKLTRLLRTKGAQNGCIIGLAAGDEVTPALVDKAVAAA
                     RTAPSMSGLDLAKVVSVTQPYDWTQGEWHLGSGYGERKAARYHVVAFDYGVKKNILRM
                     LAERGCKVTVVPARTP"
BASE COUNT         2129 a         4147 c         4271 g         2121 t
ORIGIN      
        1 cctgtgtccg gcattgcggc gagcgcggcg tgcgggtgct caccgtcttc gccttctcgt
       61 cggagaactg gaatcgcccc gccgaggaag tctccagcct gatggaactg ctggccatgg
      121 cgctggcgcg ggaggtgccg caactcaaga cagagggcgt gcgcctgcat ttcgtcggcg
      181 accgggtagg gcttgccgac aaggttcggg ccggcctggc ccaggcggag gcggacacgg
      241 ccggcaacga ccggctgatt ctgaacgtgt gcttcaacta cggcgggcgc tgggacatcg
      301 cgcaggccgc ggcacggctc gcggcacggg gcgagcccat cacggaacaa agcatgggca
      361 ccgcactggc gctggcgcac gttcccgacc ccgacctggt gatccgcacc ggcggcgaac
      421 ggcgcctgag caatttcctg ttgtggcagg ccggctacgc cgaactgtat ttcagcgaca
      481 agctctggcc cgagttcgat gcggcagcgc tcgacgaggc cttgtccgac tacgcgacgc
      541 gggagcgcag gttcggcaag acgccggagc agttgtccac ccatgacgcc aagaaggtcg
      601 cctgacagcc gcgggcaatc ccatgctcaa gcagcgcatc atcaccgcgg tcgtcctgct
      661 ggcgatactg ctgccggcgc tgttctggcc ttcgcctgtt cccttcatgg cggtcgcgct
      721 catcctcatc ggtgcgggcg cgtgggaatg ggggcgactg aatggcttgg cgcagacagg
      781 cgcattgctg cttgccggcg tgtgcgtggc actatgcgcg gcctcctgga acacagtgct
      841 gttgcccggg ccgttgcctt tcgtgtggtt cttcgcaggc gtggtgtggg tcatgggcgg
      901 agcctggctg ttgcgcgtcg gcgtggcggg ctggcctcga attccccgga tggtgcggct
      961 tgcagccggc gtgcttgcgc tctggctggc gtggctggcc gtggcaaagg ccagggttgt
     1021 cggcatcaat ttcctgctgt cggttctggt tctggtttgg gtggccgaca tcgccgcgta
     1081 cgccgcgggc cgcaccttcg gcctgaagtt cacccggaac agactggcgc cgtccatcag
     1141 ccccggcaag agctgggaag gcgtttgggg cgggatggcc ggggtgctgg tgctggcggt
     1201 cgcgtgggtc ctcgccgacc gggcattgca ggcgggggtg ccgagcctct atacgcggct
     1261 tgctgacgcc ggatggtggc tgctcgtgat cggggccgtt ttcctggcgg ccatgagcgt
     1321 ggtgggggac ctggtcgaat cgctgatcaa gcgcagcgcg ggggtcaagg actccagcaa
     1381 cctgctgcct ggccacggcg gcgtgctcga ccgtgtggat gcgctcctgc cggcgttgcc
     1441 gctggcaatg atgcttacca cgttggcgta ccgatgaagc agcgcatcac agtcctgggc
     1501 tccaccggtt cggtaggcgc caacaccctg gacgtgatcg cccggcatcc cgggcgcttc
     1561 gaggtcttcg cgctcggcgc cgccacgcag gtcgatctca tgctgcggca gtgcgcccgg
     1621 ttcaagccgg cgttcgcggt gatgggcagc gaggcggccg ccgcggagct ggcgcgaaaa
     1681 atcgaagccg gcgggctggc cacccgggtg cgttgggggc aagccgcgct ggaagagatg
     1741 gcctcccacg aattggtcga tacggtgatg gccgccatcg tcggcgcggc aggcctcgct
     1801 tcatgcctgg ctgccgcgcg tgccggcaag cgactgctgc tggccaacaa ggaggcactg
     1861 gtggtgggcg gcgagctgtt cctcgcggcg atgaagcagg gcggggccat gctgctgccg
     1921 atcgacagcg agcattcggc catcttccag tccttgcccg aagacgccgc gagctgggag
     1981 gcccgggtcg aaaaaatcat tctcaccgcc tcgggcgggc cgtttcgcac ccgcgagccc
     2041 gcgaccctgc gcaacgtgac gcccgaggag gcttgcgccc accctaactg ggtgatgggg
     2101 cgcaagatct cggtggattc cgcgaccatg atgaacaagg cgctggaggt gatcgaggcg
     2161 cgcttcctgt tcggactggc ccccgagcgg ctggaggtgg tgatccatcc gcaaagcgtc
     2221 atccattcga tggtgcagta ccgcgacacc tcggttgtcg cccaactcgg cacgcccgac
     2281 atgcgcgtgc cgatcgccta cgggctggcg tggcccgacc gcgtcgtctc cggtgcgcag
     2341 gccttggact ttcacacgct ggcggacatg agcttcgaat cgctggacag ccggggccac
     2401 cgcgagcgct tcccgggcct ggcgctggcc tgggagacgc tgcgcgcacc gcccgggacc
     2461 actgccgtgc tcaacgccgc caacgaggaa gcggtggcgg ccttcctgga ccggcgcatc
     2521 cgcttcgacc agatccacga ggtgaatgtt gcaactttgg aagcggttac agcgtccaat
     2581 cccgaatcgc tggaagatct gcttgacctg gacgcgcgtt cgcgttcggc cgctgcccgg
     2641 gctatcgccc ggctgctctc gtgaactgac tgttatcgat gctgaccatt gttgctttca
     2701 tcgtcgcact gagcctgttg gtcgcggtcc acgaatacgg ccactaccgg gtggccgtgg
     2761 cctgcggcgt gaaggtgctt cgtttttcgg tcggcttcgg caaaattttg taccgctggc
     2821 aaccgaaacg gcggaaaccg ggacagagta cggagttcgt catagggctg tttccgctgg
     2881 gcggctacat caagatgctg gatgagcgcg aagggccggt cgcgccggaa gagcggcacc
     2941 tggccttcaa cacgcagccc ctgaaatcgc gcacggcgat cgtcgcggcc gggccgatcg
     3001 ccaacctgct gctggccgtc gtgctgttcg ccaccgtcaa ctggtatggc gtcgaggaac
     3061 ccaaacccgt tcttgccagc ccggcgccgg gttcgctggc tgaacgcgcc ggcctgcgcg
     3121 gcggcgagga ggtcgagcaa gccggattcg acggcgaagc gctgcattcc ttgcgctcct
     3181 tcggcgagct gcggtggctg ttgacgcgtg gcgccgtcga tcgacgcgac gtgcgcctgc
     3241 aactcgcgcg tgaccgccag ggtgcgcggg ggcaggcact gctggcgttg agccaggtgc
     3301 agtccgccga cgtcgacgcc agcctgtccc gcaagatcgg catcatgggc ccctggacac
     3361 cgcccgcgat cggcgaggtc atggccggcg gcgccgccga gcgcgccggc ttgcgcaagg
     3421 gcgacctcgt cctgtccatc gggcaaacgc gaatcgtcga cggccaacag ctgcgcgaga
     3481 tgatccgcga cagcgccaag gccggcaagc ccggggcaga gaaatggcaa gtgcgccgcg
     3541 gtgggcagac gctggagctg ccggtggtgc ccgacgccgt gcagcaaggc gaaaccgtca
     3601 tcggccgaat cggcgcctat gtcggcgcgg cgccggagct cgtcaccgtt cgctacggac
     3661 ccctggaagg cctgtggaag ggcgtcgagc gcacctggga agtctcggtg ctgaccctgc
     3721 gcatgatggg caggatggtc atcggcgaag cttcgctgaa gaatctgagc gggccgctga
     3781 cgatcgccga ctacgcgggc aaatcggcca gcatgggcct gacgcagtac ctggtgttcc
     3841 tggctctgat cagcgtcagc ctgggtgtgc tgaacctgct gccgctgccg gtcttggacg
     3901 gcgggcacct gatgtattat ctttgggagg ctgttacggg caggggcgtc tccgacgcct
     3961 ggatggagcg cctgcaacgt ggcggcgtcg ctgtgctgct ggtcatgatg tcgatcgcgc
     4021 tgttcaacga cgttgcccgt ctctttggtt aaccttttgt ggctgccgtc accgccgcgt
     4081 tgaggggtgg gcgcctacct tgattgatga aaaaacaaat aaagcgcttt cgcgtgcgtg
     4141 ctgtttcggc catggtggcg gtggcattcg tggccaatgc ctgggcggtg gatccgttca
     4201 cagtgcgcga catccgcgtg gaagggctgc agcgggtcga gccgggcacg attttcgcct
     4261 cgctgccgtt ccgtatcggc gaccagtaca acgatgaaaa ggggtccgcc gcgatccggg
     4321 cgctgttcgc gctgggcctg ttcaaggacg tccggctcga agtcagcggc gacgtgctgg
     4381 tggtgattgt cgaggaacgt ccgacggtgg ccgacgtgga cttcgtgggc gccaaggaat
     4441 tcgacaagga cgcgctgaag aaggccttgc gggagatcgg cctgaccgag gggcgcccct
     4501 acgaccaggg actggcggac cgcgccgaac aggaactcaa gcgccagtac atcaacaaga
     4561 gcctctacgg cgccgaggtc gtgaccaccg tgacgccgat cgagcgcaac cgcgtcaacc
     4621 tgaccttcac cgtcacggag ggcgagccgg cacgcatcaa ggaaatccgg gtcgtcggca
     4681 accgggcttt cagcgaatcc acgctcaagg gcctgttcga cctggacacc ggcggcttcc
     4741 tcagctggta taccaagtcg gaccgctact cgcgcgccaa gctcaatgcc gacctggaag
     4801 cgctgcgctc gtattacctc agccgcggct acctcgaatt ccgcatcgat tccacgcagg
     4861 tcgcgctctc gcccaacaag caggacatca cgatcaccgt gaacgtcacc gaaggcgagc
     4921 ggtatgtggt ctccggcgtc aagctcgaag gcaattacct cggcaaggaa gaagaattca
     4981 agtcgctcgt gacgatccgg ccgggccagc cgttcaatgc ggaccaggtc gccgaaacca
     5041 ccaaggcctt caccgactac ttcggcaact tcgggtatgc attcgccacc gtggaggcgc
     5101 gcccggagat cgaccgcgcc aacaaccgcg tggcgttcgt gctgctggcg gagccctcgc
     5161 gccgcgccta cgtgcggcgc gtcaacatcg ccggcaacaa ccgcacgcgc gatgaagtga
     5221 tccggcgcga gttccgccag ttcgaggcct cctggtacga cgccgacaag atcaagctgt
     5281 cgcgcgaccg gatcgaccgg ctcggcttct tcagggaagt gaacatcgaa accagcgacg
     5341 tgccgggtgc gcccgaccag gtcgacctga acatcaccgt ggtcgagaag cccaccggca
     5401 gcctgcaact gggggcgggc ttttccagtg cggaaaagct ggccctgtcg ttctccatca
     5461 agcaggagaa cgcgttcggc accggcaact acctgggcgt cgaggtcaac accagcaagt
     5521 acaaccgaac ggtcgccgtg acatcggtca acccgtactt cacggcggac ggcatctcgc
     5581 gcacggtcga cctttaccat cgcacgtcgc ggccgtacga agaccagggc ggcaattacc
     5641 agctggccac caccggcgcg gggctgcgct tcggggtgcc cttcagcgag ctggacaccg
     5701 tgtatttcgg cggcagcgtt gagcgcaccg agatcaggcc cggcaccaac atcccggcgg
     5761 cctacctggc gtatgccgac cggttcggct tcaccagcag ctccatccca ttgtcgatcg
     5821 gctggtcgcg ggatagccgc gacagcgcca tcgcgccgaa ttccggccgc taccagcggg
     5881 tcgcgggcga ggtcggggtc ggaggcgacg cgcgctacct gcgcgccaac taccagttcc
     5941 agcaatacgt tccgctcaac aagcagtaca ccctggcctt caacggcgag gccggctggg
     6001 gcaaaggcct gggcggccgg ccgtatccgg tcttcaagaa cttctactcg ggcggcctgg
     6061 gctcagtgcg cggcttcgac cagggcaccc tgggccccag ggacgtcacc ggttcctcca
     6121 ttggcggccc caagaagatc accctgaatg ccgaagtgat cacgccgttc ccaggcgccg
     6181 gcaacgaccg caccctgcgg ctgttcggct tcgtcgatgt gggcaacgtt tttggcgagc
     6241 atgaaaagat ccactttggc gacatgcgcg cctcggtcgg tgtcggactg tcctggatct
     6301 cgccgatcgg gccgctgcgg ctggcggtcg cccaacccat ccgcaagttc gctggcgata
     6361 gaatccagag attgcaattc cagatcggaa cgtctttcta atgaagtacc tgtcccgtca
     6421 atggctggtg atcttcctgg gtgtgctggc actggccgcc ggggcgcacg cgcaggactt
     6481 caaggtcggc ttcgtcaaca ccgaccgtat cttccgtgaa gccaataccg ccaaggccgc
     6541 ccaggccaag ctggagcagg agttttccaa gcgcgagaag gaactcaacg acctgggcaa
     6601 cggcttgaag atgctttccg acaagttcga gcgcgaggcg cccaccttgt cggaatcgca
     6661 gcgcgcccag cgccagaagc agatcctgga ccaggaccgc gacttccagc gcaagcgccg
     6721 cgagttccag gaggacctga acgcgcgcaa gaacgaggaa cttcagcagg tgctggaacg
     6781 cgccaatcgc gtggtcaagc aggtcgcgga agcggaaaag tacgatgtgg tgctgcagga
     6841 ggcggtctac atcaatccca ggcacgacat caccgacaag gtgatcaagg cgctcaacgc
     6901 ctcgcggtag tgcaactccg attaggctcg attgttgaaa cgctcggcgg cgagctttgc
     6961 ggcaatcccg agcgtctgat cgagggcctg gcgccgctgg agtccgccaa cccggcccag
     7021 ctgagtttcc tcagcaatcc caagctggcg cagcagctcg ccacctcccg ggcggcctgc
     7081 gtcatcgtgg cgcccgccct gcgtgaagcg gccctcgcgc gcggcgactg catcgtcgcc
     7141 gagcagcctt acctgtattt cgcccgcgtg acccagctgt ggaagaagcg gctcggcgcg
     7201 gccgagggtc cgctgattca ttccactgcc gtcatcgacc gcgaagccgt ggtcgacccc
     7261 agcgcgcgca tcgggccact ctgcgtgatc gcgcgcggcg cgcgcatcgg cgccgggacg
     7321 gtcttgaagg cccgcgtcag cgtgggcgag gcctgcgtca tcggcgaccg ctgcatcatc
     7381 caccccgggg cggtgatcgg agccgacggt ttcggcttcg cgcccgacgg aacgggcggc
     7441 tgggaaaaga tcgagcagct cggcgcggtg cgtatcggca acgacgtcga gatcggcgcc
     7501 aacacctgca tcgaccgggg tgcgctgcag gataccgtga tcggggacgg cgtgaagctg
     7561 gacaacctga tccaggtcgg ccacaacgtg cgcatcggca ggcacaccgc catcgccggt
     7621 tgcgccggca tcgcgggcag cgcgacgatc ggggcctact gcaccatcgg cggggccgcc
     7681 atgatcctgg gccacctcag gctggccgat cacgtgaaca tttcggccgg cacgttcgtc
     7741 ggccgctcga tcctcaagcc cggtcactac accggcatct ttcctatgga cgacaatgcg
     7801 cagtgggaga agaacgcggc aacgctcaag cagcttcaca agctgcgcga tcgcgtgaag
     7861 gccctcgaga agaagaccga accatgatgg acatccacca gatcctcaag catctgccgc
     7921 atcgctatcc tttcctgatg gtggaccggg tgcttgagat cgacaagggc aagagcatcc
     7981 ggacgctcaa gaacgtgacg atcaacgagc cgtactttgc cggccatttc ccgcatcggc
     8041 ccgtgatgcc gggtgtgctg atgctcgagg ccatggcgca gagcgcggcc ctgttgtcgt
     8101 tcgccaccat gggcgtcatg cccgacgaca agaccgttta ctattttgcc ggtatcgacg
     8161 ctgcgcgttt caagcgaccc gtggaaccgg gggaccagct tctgatggat gtgacgctga
     8221 ttcgctcgaa ggccggcatc tacaagtaca agggcattgc gcgcgtgggc gaggagctgg
     8281 cgtgcgaagc cgagctcatg tgcaccatgc gcactgtcgg ctagtccggg ccccggcaat
     8341 gacacggatc catcccacgg ccattgtcga tcccggggcc gagctcgacg ccacggtatc
     8401 ggtcgggccc tatacggtca tcggtgcgca tgtgaggata gccggcggca cgacggtggg
     8461 cccgcactgc gtgatcgaag gccgcaccac catcggccgc gacaaccgca ttttccagtt
     8521 cgcatcgctg ggtgcgatac cgcaggacaa gaagtacgcg ggcgagccct gcaagctggt
     8581 gatcggcgag cgcaacacca tccgcgaatt cagcacatac aacatcggtt ctcccggagg
     8641 cggcggcatc acgcgcctgg gcaatgataa ctggatgatg gcgtacgtgc acctggcgca
     8701 cgactgcatc gtgggcgacc atacgatctt cgccaataac gcgcagctgg ccgggcatgt
     8761 cgaggtgggc gactgggcga tccttggggg ctttaccacc gtgcaccagt tcgtccggat
     8821 cggggcccac ggcatgacgt cgctcaacgc gatgctcgtg gccgaccagc cgcccttcgt
     8881 gatgagccag ggccagccgg cgcaggcgcg ctcgatgaac ttcgagggct tgcgccggcg
     8941 cggattcgcg gcggaacgca tcgccgccgt caaggccatg cacaaggccc tgtatcgcga
     9001 cggccttacg ctcgagcagt ccaaacagcg catcgccgca ctggcggaga aaacgcccca
     9061 ggccgcgccg gacgtccaga tgatgctgtc gttcctggat cgggtttcgc cgcagcgcgg
     9121 cattgtgcgt tgactccgcg catcgccatg gtcgcggggg aggcgtccgg cgacctgctg
     9181 gcgggactgc tgctggacgg cttgcgccgg cggtggcccg gcttgcaagc cgcgggcatc
     9241 ggcggtcccc agatggcgcg gcgcggcttc gaagcctggt ggccccacga aaagctggcg
     9301 gtcagcggct atgtggaggt gctgcggcac taccgcgaga tcgtcggtat tcgcgagcag
     9361 ctcaggcggc gcctgctcga gcatccgccg cagctcttca tcggcatcga cgcacccgac
     9421 ttcaacctgg acctggaggc cgccttgaag tcgcgcggca tccggatcgt gcacttcgtg
     9481 tgcccgtcga tctgggcctg gcggccgcag cgcgccgcga agctcaaagc gagcgccgac
     9541 catgtgcttt gcgtctttcc cttcgagccc gagttgctgg cgcgccacgg catcgagtcg
     9601 acctacgtcg gccacccgct ggcgagcctg atcccgatga agccggataa ggccgcggcc
     9661 cgccgcgccc tggggctgga ggcggccggc gaagtgctcg cgatcctgcc gggcagccgc
     9721 gcatcggagg tgcaccgcct ggcggggcgc ttcctcgcag ccgcggcgct cgtgcttcga
     9781 aagcgcccgg gaatgaagct ggtcgtgccg gcgctgcccg cccggcgcga agcggtcgag
     9841 gaagccgcgc gtgccgcggg cctcggggac ggcctgcaaa tcgtgcaggg ccagtcccac
     9901 acggtgctcg cggcatgcga cctcgcgctg gttgccagcg gcacggcgac cctggaggcg
     9961 gcgctgttca agcggccgat ggtgatcgcc tatgcggtga actggttgag ctaccggatg
    10021 atgaagccca gacacctgca gccgtggatc gggctgccca acattctgtg cggcgaattc
    10081 gttgtgcccg agctcaccca gcacgatgcg aatcccgagt cgctcgctgc ggccctcttg
    10141 gattggctcg aagctcctgc cagaatggcg gccgtacagg agaaattcac cgcgttgcat
    10201 gcgctgctgc aacgcgatac ggcaacactg gccaccgatg ccatcgaaaa agtcatccag
    10261 ggctgaagcg gcggccccgc ccaagccgat ccgcgtgcga gcggcgcgcg gtgcagccag
    10321 cattcaggcg cgcctgccgt gggaccccat cgggctgatg gccggtgtcg acgaggccgg
    10381 acgtggcccg ctcgccggac cggtggtggc cgccgccgtc attctcgacg acaccaagcg
    10441 tatccgcggc ctggccgact ccaaggtgct gacgccgctg cagcgggatc gcctgtacga
    10501 ccagatccgc gagaaggccc tgtgctgctc ggtggcgcag gccagcgtcg aggagatcga
    10561 tacgctcaac atcctgtggg ccaccatgct cgccatgaaa cgggcggtcg aaggcctgcg
    10621 gttgaagccg gccaaagtcc tggtggacgg caaccgcctg ccggtggtgg acatgctggc
    10681 cgaggcggtg atcggcggcg atgccacggt gaaggcgatc tcggcggctt ccatcctggc
    10741 caaggtgcat cgcgaccgtc tctgccaggc gctgcatgcg gaattcccac agtacggctt
    10801 tgcctcgcac aagggttacg ccacacccga gcacctggag gcattgcggg tgcatggcgc
    10861 ctgccaccat caccgcaggt ttttcggtcc ggtcgcggct actttcgcgc tcggggatgg
    10921 cgcggtcgcc gacttcgctc tccgggatga ggctgcagcc gcgttcgcca tcgaggaagt
    10981 gggggtggtg gtggccggca tcgtcgcgtg acctccagcg atccccagtt cgtcagctcg
    11041 gcggccaatg ccctgctaaa ggacctgcgc cggctggcgc aggacagctc ggcctatcgc
    11101 aaacaaggcc gcgtctggct ggagggcgat cacctgtgcc gcgccgcgat ggcccgggga
    11161 gtgcaacccg cactggcggt tttctccgaa tcgtcatggt ccgccggcga gtccgcatgg
    11221 gccggccgag ccggaaagca cgttgtcatt gccgacgccc tgttcgcagg gttgagcggc
    11281 ctcgaatccc cggctcgcat gggattcgtc gtggacttgc cgccggcgcc ggcgctgcgg
    11341 cccgacgtct gcacggtcat tctggaccgc gtgcaggacg cgggcaacgt cggggcgatc
    11401 ctgcgttgcg ccgccgcgtt cgggttcggc caagtcctcg cgatcaaagg gaccgcggcg
    11461 ctgtggagtc ccaaggtgct gcgatccggc atgggcgcgc atttcggctt gcggctgatc
    11521 gaaagcctgg cgccggaggc gctggatgcg ctggcgcttc ccatcatcac gaccagttcg
    11581 caccaaggcg agctgcttca caggcgagaa ctcccctggc catgcgcctg ggccctcggc
    11641 catgaagggc agggcgtcag cccggaaatc gcggcgcgct cacgcctggc ggttcgcatc
    11701 gtccagccgg gcggcgagga gtcgctgaac gtcgcctccg cggcggcgat ctgcctgcac
    11761 gccagtgccg cggcccatgc cgggtgacgt tttgcaaagg cctttgcact gcgggaattg
    11821 aaggccctca gctataatca agagctttcc cgcaaacccg tacgcctagc gcatccaagc
    11881 caaatccctg aaaaaagctg ccgcgagagc ccgctcttgc ccgcgcactt gatacctggg
    11941 cgtacccgta acaatccgga gaacccagtg cttttgcctc atcagggaac tttccctccc
    12001 gccatcctgg cgctcgcgga cggcacggtc tttcttggca actcgatcgg ggccacgggc
    12061 tccaccaccg gagaagtcgt gttcaacacg gccatgaccg gataccagga aatcctcacc
    12121 gacccgagct actgccagca gatcgtgacg ctcacgtatc cgcacatcgg aaactacggc
    12181 gtcaacgaag aagacgtcga ggcctgcaag atccacgccg ccggcctgat cattcgcgac
    12241 ctgccgctgg tcgcttccaa tttccgcacc agccagtcgc tgtcgcaata cctgcgcagc
    12301 cagaacacgg tcgccatcgc gaacatcgac acgcgcaagc tcacgcgcct gctgcgcacg
    12361 aaaggcgcgc aaaacggctg catcatcggc ctggccgcgg gcgacgaagt gacgcccgcg
    12421 ctggtggaca aggccgtcgc ggccgcacgg accgcgccct cgatgagcgg cctggacctc
    12481 gccaaggtgg tgtcggtcac ccagccttac gactggaccc agggcgaatg gcaccttggt
    12541 tcgggctacg gcgagcgcaa ggcggccagg taccacgtgg tcgcgttcga ctacggcgtg
    12601 aagaagaaca tcctgcgcat gctcgccgag cgcggctgca aggtgactgt cgtgccggcc
    12661 cggacccc
//
LOCUS       DASWIY010000210        18045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2713787, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000210 DASWIY010000000
VERSION     DASWIY010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 18045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18045
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2713787"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1409)
                     /locus_tag="VGA59_07655"
     CDS             complement(177..1409)
                     /locus_tag="VGA59_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HEX9719593.1"
                     /translation="MKIAIVGAGIGGLSLALSLHREGLHCDVYETVAAVREIGVGITL
                     LPHAMRELAELGLQPQLEAAGIENFESVFYSRFGQFIYWEPRGRHAGYQWPEVGIHRG
                     KLHRVLYEAVRERLGAGRVHLDHRCVGVDQDAGGATLRFCSGNGAPLPPLRADVVVAC
                     DGINSAVRRQFYPDEKLAFGGINIWRGVTLHAPILSGKSYMRIGSLETGKMVIYPIAD
                     NADGHGRQLINWVAEIRREGASMNDWNQPGRARDVLPLFKDWRFDWLDVPGLIRNAEH
                     IFEYPMVDKDPVPRWTFGRVTLLGDAAHPMYPRGSNGSAQAIIDARVLARQLATAVDG
                     PAALAAYQALRLAVTAKIVEANRTVPPDFINSKVEELSGGKPFRHIDDLISQEQLRRI
                     SDDYKRIAGFSLEPLSDL"
     gene            complement(1432..2418)
                     /locus_tag="VGA59_07660"
     CDS             complement(1432..2418)
                     /locus_tag="VGA59_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9719594.1"
                     /translation="MLAHPWRKAAAMVIAALAAFGAAAQSPSPGAPAQGPLRLIVPFT
                     PGTGIDLIARTVGPKLGERLGRSVVVDNRAGASGNIGTEAVVRSPPNGSTLLVSVSTV
                     VMNRSLYPQLPFDPVKDLVPVSLTSWGQLLLVANPRAGYKTPADFMGAAKRRPGAINY
                     ASPGVGTPHHLAMELLKAKAGVFVTHIPYRGTAPALTDLLGGQVDVMFLPIHVALPHV
                     KAGRLVALGIGSERRHSLLPEVPTLIEARAGDITVGMWYGIFAPPGTAPDVVARLNRE
                     LKDIIATPEVRAAFETQGMDPATSTPEEFRRLVEQDADRWAQLIKAQNIRAE"
     gene            2509..3000
                     /locus_tag="VGA59_07665"
     CDS             2509..3000
                     /locus_tag="VGA59_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9719595.1"
                     /translation="MTATLALPASLSRLYMRPGFLLRRAHQISAAVFEDECRELALTP
                     AQFGVLTVLQVHPGLGQSSLARALGYDKVTVLRVLRGLETRGLVTRAPAAGNKRNVSV
                     ALTARGASVLALAQKPAEKAYKRLLAPLDKAQQAQLLALLQLLTGELENDARAAFVPP
                     ATR"
     gene            complement(3008..3517)
                     /locus_tag="VGA59_07670"
     CDS             complement(3008..3517)
                     /locus_tag="VGA59_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX9719596.1"
                     /translation="MHSRVSIQTQDFNLADEVAALRLGDKRVGAVCAFVGTVRDRNAQ
                     GPQAGDGATAAAAPVVSMELEHYPGMTEKAIEAMIDEAQRRFGIYAARVIHRVGLLHP
                     LDQIVMVAVTSAHRGESFEACEFLMDYLKTQAPFWKKERTPEGGRWVDARVSDDAALA
                     RWSIPSGNT"
     gene            complement(3572..3823)
                     /locus_tag="VGA59_07675"
     CDS             complement(3572..3823)
                     /locus_tag="VGA59_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX9719597.1"
                     /translation="MKLTVKYFASIREALGHGSETVDTSAASLAALRDELVARGGAYA
                     QSLARGKAVRVALNQVMSNESAALSDGCEVAFFPPVTGG"
     gene            complement(3820..5067)
                     /gene="glp"
                     /locus_tag="VGA59_07680"
     CDS             complement(3820..5067)
                     /gene="glp"
                     /locus_tag="VGA59_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX9719598.1"
                     /translation="MNAPSAAGAGRSALRPLDDALAELLGHAHPLAGTETVSTFEADG
                     RVLAQDLVSELQVPPLDNSSMDGYAVRSHEIADEGVPLPVSQRIPAGAAPGPLAPGSV
                     ARIFTGAPIPAGADAVVIQEDTEPAGEQIVRILRVPQPGQWIRRSGEDVARGSVVLAK
                     GDRMSPAALGLAAGIGMDRLVVARRPKVALFSTGDELVMPGEVPTAQMKPGAIYNSNR
                     FFLRALLQRLGCEVSDLGIVPDRRDATVEALRRASIDQHLILTSGGVSVGEEDHVKPA
                     VQSLGSLDLWQIAIKPGKPFAYGKVGPTHFIGLPGNPVSSFVTFLLLVRPFLLKLQGA
                     TRLAPAAVPLPAHFSLPRADKRREFLRARRNPAGGLDLFPNQSSAVLTSAVWSDGLVD
                     NPAGRAIARGETVSFISLAELIA"
     gene            complement(5064..5597)
                     /gene="mobB"
                     /locus_tag="VGA59_07685"
     CDS             complement(5064..5597)
                     /gene="mobB"
                     /locus_tag="VGA59_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX9719599.1"
                     /translation="MKVVGFAGFSGSGKTTLVERLIPALKLRGLRVSVVKHAHHKFDI
                     DHPGKDTYRHREAGAFEVVVASNQRLALIREFEQPAKLTAHHLIAELYEGVDWVLVEG
                     FKGSDLLKIEVWRDAIGKQALYPEDDFIVAIATDSPDRLPQPTLRPVLDLNDADAVVE
                     WLTDNGARFDYDPEMYA"
     gene            complement(5604..7019)
                     /gene="thrC"
                     /locus_tag="VGA59_07690"
     CDS             complement(5604..7019)
                     /gene="thrC"
                     /locus_tag="VGA59_07690"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX9719600.1"
                     /translation="MLYMSTRGHADRKHFCEILLEGLAPDGGLYLPERYPHVDDATLS
                     AWRGLPYADLAFEILSLYIDDIPAAHLRALCRKTYTRDVFGTEAIVPLKKLEDGLYLE
                     SLSNGPTLAFKDMAMQLLGNLFEYELARRGEELNILGATSGDTGSSAEYAMRGKKGVR
                     VFMTSPEGRMSPFQQAQMFSLQDANIHNLAIEGVFDDCQDIVKAVSNDLEFKRRFRIG
                     TVNSINWARLLAQVVYYFAGYFQATRSNGDKVSFAVPSGNFGNICAGHVARMMGLPIE
                     RLVLATNENDVLDEFFRTGVYRVRSGADTHETSSPSMDISKASNFERFVFDLLGRDAA
                     KVGHLFGELLPREGRFDLSADPAFRQASARYGFISGKSTHADRVATIRDTYQRFKLII
                     DTHTADGLKVARRHTQAGVPMIVLETALPIKFAETIVEALGREPDRPARFEGIEQLPR
                     RVTVMPADAQAVKEFMARHCA"
     gene            complement(7019..8350)
                     /locus_tag="VGA59_07695"
     CDS             complement(7019..8350)
                     /locus_tag="VGA59_07695"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX9719601.1"
                     /translation="MKAIQVGVLGIGTVGSGVFNVLQRNQEEIRRRAGRGIDITMVAD
                     LDTARARSVVGEGVKVVDDARAVIANPEIDIVVELIGGYGIARQLVMEAIEAGKHVVT
                     ANKALLAVHGTEIFAAAHRRGVMVAFEAAVAGGIPIIKALREGLTANSIEWIAGIING
                     TTNFILSEMRDKGLDFDVALKEAQRLGYAETDPTFDIEGVDAAHKATIMSAIAFGIPV
                     QFDKAYVEGITKLGAQDIRYAEQLGYRIKLLGITKRSFDKATGALKGIELRVHPALVP
                     AKRLIANVEGAMNAVLVQADAVGTTLYYGKGAGSEPTASAVIADLVDITRLHTADAAH
                     RVPHLAFQPDSMSDTPVLPMSEVVTSYYLRLRVADQAGVLAKVTGQLAAAGISIDAML
                     QREAGEGEKQTDLIILTHSVREGTMNDVIAQMQGLPTVLAPITRIRKEELA"
     gene            complement(8347..9573)
                     /locus_tag="VGA59_07700"
     CDS             complement(8347..9573)
                     /locus_tag="VGA59_07700"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9719602.1"
                     /translation="MKTVQKSAKLANVLYDIRGPIMDAARQMEEEGHKIIRLNIGNLA
                     VFGFDAPEEIQQDMIRNLPNSSGYSDSKGIFAARKAVMHESQKQGIKGVTLDDIYLGN
                     GASELIAMATNALLDDGDELLLPAPDYPLWTAAASLSGGTPVHYLCEEANGWMPDLQD
                     IRRKITPRTKGIVVINPNNPTGALYSDDLLKEIVQIAREHNLVIFADEVYDKVLYDGA
                     RHTAIASLSEDVLTLTFNSLSKSYRSCGYRAGWMIVSGDRKPAADYIEGLNMLSNMRL
                     CANVPGQYAIQTALGGYQSINELVGEGGRLRRQRDLAYELITAIPGVTCVKPTAALYM
                     FARLDPKVYPITDDRQFFLELLQETRVMLVQGTGFNWATPDHFRIVFLPHEDDLREAV
                     GRIAKFLENYRKRTTK"
     gene            9678..10055
                     /locus_tag="VGA59_07705"
     CDS             9678..10055
                     /locus_tag="VGA59_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEX9719603.1"
                     /translation="MKLQPDQSDVQSISGYGPGWVGVNGEKITRSVIITSGGARLDWA
                     VDRFEDLGADHFAQLALVEAEVVIFGSGSRIRFPDAAWLRPLIARRIGVETMDTAAAC
                     RTYNILAQEGRSVAVALLLEQPA"
     gene            10163..10639
                     /locus_tag="VGA59_07710"
     CDS             10163..10639
                     /locus_tag="VGA59_07710"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9719604.1"
                     /translation="MAIVVNKPIPEFEANATGGIKVTNTSHNGQVLVLYFYPKDNTPG
                     CTTEAMQFRDKYKDFVKAGAAVFGVSRDNMKSHDDFKAKLELPFELIADTEEKMCHMF
                     GVVKNKIMYGKKVKGIERSTFLIGADGVLKQEWRGLKVPGHVDEVLKAVKLLKKAA"
     gene            10816..12501
                     /locus_tag="VGA59_07715"
     CDS             10816..12501
                     /locus_tag="VGA59_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX9719605.1"
                     /translation="MPLPPAPTKRAALLPPEAYDAPARSSHKAARKSEANTGSPLAEK
                     PLVLDFDPRQGGGELHPEAFKDPTDGYFDHQESAVHKRRLAPEPAPRPARKVKPAGPS
                     KLFVLDTNVLMHDPMSLFRFEEHDIFLPMIVLEELDGHKKGMTEVARNARQASRSLDA
                     LAGTQGADIASGLKLDTTGHREAGGCLFFQTRQLDSTLPLGLPQGKADNQILGVVQAL
                     REQHAPRDVVLVSKDINMRVKARALGLATDDYQNDKTIDDLELLYSGALALPSDFWAK
                     HGKTIESWQSGLHTYYRITGPVVPSLLINQFVYFESPGEPSLYARVTEIRAKTAVLKT
                     LKDYGHLKHSVWGVTTRNREQNFAMNLLMDPEVDFVTLTGTAGTGKTLMALASGLTQV
                     LDDRRYTEIIMTRATVSMGEDIGFLPGTEEEKMGPWMGALDDNLEVLAKTETGAGEWG
                     RAATNELIRSRIKIKSMNFMRGRTFLNKYVIIDEAQNLTPKQMKTLITRAGPGTKIIC
                     MGNLAQIDTPYLTEGSSGLTFAVDKFKGWPHSGHIMLARGERSRLADFASEVL"
     gene            complement(12521..13174)
                     /locus_tag="VGA59_07720"
     CDS             complement(12521..13174)
                     /locus_tag="VGA59_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9719606.1"
                     /translation="MLKALFSSLMASPAQTAGKESLAPAGLPLRLNVGCGRNILDGWV
                     NLDIAALPGVDIVADLDNCRESPLPFDDDSFDEFLLSHVIEHIRDPLALMQELHRIAR
                     PGARMVLRVPHGASDDAFEDPTHVRQYFHGSFGYFSQPFYWRADYGYRGDWLTGVIRL
                     KVERTRFPDATAAEILVRINRERNVILEMEAELTAVKPARAPDRSLQVPSDIRIAFA"
     gene            complement(13183..14589)
                     /gene="dnaB"
                     /locus_tag="VGA59_07725"
     CDS             complement(13183..14589)
                     /gene="dnaB"
                     /locus_tag="VGA59_07725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX9719607.1"
                     /translation="MSAVLTSVDDDFSRDHQIAQLRIPPHSIEAESSVLGGLLLDNGA
                     WDRMGDLLTDGDFYRYEHRLIYAAIGGLINATKPADVITVHEQLQGLGKADEVGGLAY
                     LNSLAQYVPSASNIRRYAEIVRERSILRKLVSASDEIATAAFNTQGKPVDKILDEAEQ
                     KIFNIGEEGSRMKQGFQAMDSLVVQLLDRVQEMADNPNDITGVPTGFYDLDRLTSGFQ
                     AGDMIVLAARPSMGKTALAINIAEHVALHEELPVAVFSMEMGASQLAVRIVGSIGRID
                     QMHLRTGKLTDDEWPRLSEAIEKLRNVSLHIDETPGLTVSELRANARRLARQCGGKLG
                     LIVVDYLQLMSVSSSMSEENRATAVGEISRGLKMLAKELQCPVMALSQLSRGVESRTD
                     KRPMMSDLRESGAIEQDADVIMFIYRDDYYNKDSKEPGVAEVIISKQRNGPTGTVKLA
                     FVKPLTKFESLASGSSDF"
     gene            complement(14781..15233)
                     /gene="rplI"
                     /locus_tag="VGA59_07730"
     CDS             complement(14781..15233)
                     /gene="rplI"
                     /locus_tag="VGA59_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX9719608.1"
                     /translation="MQVILLDKVVNLGNLGEIVKVKDGYARNFLIPQGRARRATEANK
                     AEFEARRVELEKAAAAKLAQSQSEAEKLAGTTVKLTQKAGVDGRLFGSVTNSDIAEEL
                     NKLGYKVAKAQVRMPTGPIKHTGDSTVSVALHTDVAVDITVTVYGETA"
     gene            complement(15246..15527)
                     /gene="rpsR"
                     /locus_tag="VGA59_07735"
     CDS             complement(15246..15527)
                     /gene="rpsR"
                     /locus_tag="VGA59_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX9719609.1"
                     /translation="MATFKKFNKDKRPKRNTQSLLFKRKRFCRFTVAHVEEIDYKDVD
                     TLRDFIAENGKIIPARLTGTRAIYQRQLNTAIKRARFLALVPYTDQHRI"
     gene            complement(15619..15906)
                     /gene="priB"
                     /locus_tag="VGA59_07740"
     CDS             complement(15619..15906)
                     /gene="priB"
                     /locus_tag="VGA59_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEX9719610.1"
                     /translation="MNQLVLSARIAEAGVLRYTPAGLPALDFRLEHESEVTEAGQPRQ
                     VAVAIRAVAFGGLAETLGKQAIGSSWKFTGFLATPRNGKHPVLHIQEFQEI"
     gene            complement(15923..16294)
                     /gene="rpsF"
                     /locus_tag="VGA59_07745"
     CDS             complement(15923..16294)
                     /gene="rpsF"
                     /locus_tag="VGA59_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX9719611.1"
                     /translation="MRHYEIILLIHPDQSEQVPAMLERYKGMITAGGGKVHRVEDWGR
                     RQLAYQINKLAKAHYLCINIEADQPVMAELEHAFRFNDAVLRHLTVQRKKADTGPSAM
                     MRTVEREEARKAQQAEYQAAA"
     gene            complement(16460..>18045)
                     /locus_tag="VGA59_07750"
     CDS             complement(16460..>18045)
                     /locus_tag="VGA59_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HEX9719612.1"
                     /translation="GVGIITTRLTGVAFELGIFLGLGGILVCSFLGGMRAVTWTQVAQ
                     YIILIMAYMIPVVWLAVKQTGVPIPQAIYGYQLEKVSKRGDDLRTDPKELEVVAIFKA
                     RADEFDAKLKNIPTALATDRAAAEKKLADLKAANAMASQVTAAEKELAAVPKDEAAAK
                     DAWTKAKAANLDRAKPLAGMPNHAQVFAGDPKGTPAEQKAFDESRRNFLALIFCLMVG
                     TAALPHILMRYYTTPSVKQARDSVTWSLFFIFLLYFTAPALAVLVKYEVFNVLVGTPF
                     DKLPAWINNWQRVDPGLLSVIDVNKDGIFQLTEMKIGGDIIVLATPEIGGLPYVVSGM
                     VAAGGLAAALSTADGLLLTIANALSHDLYYKMIDPNASTARRVTLSKILLLVVALAAA
                     GVASQKPADILFLVSAAFSFAAAAFFPALVLGIFWKRATGIAASLGMASGLGITFYYM
                     VITQPWMRSVFGVTSPIELWWGILPISAGLFGVPLGFAVIILVSLFTPAPNARIQDLV
                     EHVRYPSLKGSAKSLVHGL"
BASE COUNT         3094 a         5853 c         5871 g         3227 t
ORIGIN      
        1 ggcggtcgag gatggcattt ccgccgacga ggaccgcgtc tcgcgcaagc cgcgctacct
       61 gaactgacgc gtggattgcg ggtcagtatt gggtcaagcc cgcaatgaca gagcgtcatc
      121 ccggactcga tccggaatcc atggtcgtgg tcgattgcgg cttccgtagt gacaacttat
      181 aggtcactca gcggctcaag cgagaagccg gcaatgcgct tgtagtcgtc ggaaatgcgg
      241 cgcagctgtt cctgactgat caggtcgtcg atgtgcctga acggcttgcc accgctgagt
      301 tcctcgacct tgctgttgat gaaatcgggt ggaacggtgc ggttggcctc gacgattttc
      361 gcggtgaccg caagccgcag agcctggtat gcggccagcg ctgccggacc gtcgaccgcc
      421 gtggcgagtt gccgggccag gaccctggcg tcgatgatcg cctgcgccga accgttcgag
      481 ccgcgcggat acatgggatg tgcggcatct cccaggagcg tgacgcgacc gaaggtccag
      541 cggggcaccg gatccttgtc gaccatcgga tattcgaaga tgtgctcggc attcctgatc
      601 aggccaggca cgtccagcca gtcgaaacgc cagtccttga acagcggcag cacgtcccgc
      661 gcccggccgg gctggttcca gtcgttcatg gacgcgcctt cgcggcgaat ctcggccacc
      721 cagttgatca gctggcggcc atggccatcg gcattgtcgg cgatggggta gatcaccatc
      781 ttgccggttt ccagcgatcc gatgcgcata tagctcttgc cgctgaggat aggcgcgtgc
      841 agggtcacgc cgcgccagat attgatgccg ccgaacgcca gtttttcatc gggatagaac
      901 tggcgccgca cggccgaatt gatgccgtcg caggccacca cgacatcggc gcgcagcggc
      961 ggcagcggcg cgccgttgcc gctgcaaaag cgcagtgttg cgccaccggc atcctggtcg
     1021 acaccgacgc agcggtgatc caggtggacg cggcccgcgc cgagccgctc gcgcacggcc
     1081 tcgtacagca cgcgatgcag cttgccgcga tggatgccga cctccggcca ctggtagccg
     1141 gcgtgccgtc cgcgcggctc ccaatagatg aactggccga accggctgta gaagacgctc
     1201 tcgaaatttt cgatgccggc ggcctcgagc tgcggctgca gcccgagttc ggccagctcg
     1261 cgcatcgcgt gcggcaacaa ggtgatgccc acgccaatct cccgcacggc cgccacggtc
     1321 tcgtaaacgt cgcaatgcag gccctcccgg tgcagggaaa gcgccaggct caggccgcct
     1381 atgccggccc cgacgatcgc aatcttcatg gaatcccttc gttgcgcgtc ttcactcggc
     1441 cctgatgttc tgcgccttga tcagctgggc ccagcggtcg gcgtcctgtt cgaccaggcg
     1501 gcgaaattcc tcgggcgtgc tcgtggccgg gtccatcccc tgcgtttcga acgccgcgcg
     1561 tacctcgggc gtggcgatga tgtccttgag ctcacggttc aggcgtgcca cgacatccgg
     1621 ggccgttccc ggcggcgcga agatgccgta ccacatgccc acggtgatgt cgccggcgcg
     1681 tgcctcgatc aacgtcggca cttcgggcag caaggaatgg cgccgttcgc tgccgatgcc
     1741 cagcgcgacc agccggccgg ccttcacatg cggcagcgcg acgtgaatgg gcaggaacat
     1801 cacgtcgacc tgcccgccca gcaggtccgt gagggcgggc gccgttccgc ggtaaggaat
     1861 gtgggtgacg aacacgccgg ccttggcctt cagcagttcc atcgcgaggt ggtgcggcgt
     1921 gccgacgccc gggctggcgt aattgatggc gcccggccgg cgcttggcgg ctcccatgaa
     1981 gtcggccggc gtcttgtaac cggcgcgcgg gttcgccacc agcagcaact gcccccagct
     2041 ggtcagcgat accggcacca ggtccttgac cgggtcgaac ggcagttggg gatagaggct
     2101 gcggttcatg accaccgtgc tgacactgac cagcagcgtc gaaccgttgg gcggcgagcg
     2161 caccacggct tcggtgccga tgttgcccga ggcgcccgcg cggttgtcca cgacaacgga
     2221 gcggccgagc cgctccccca gcttgggccc caccgtgcga gcgatcaggt cgatgcccgt
     2281 ccccggcgtg aagggcacga taaggcgcag aggcccttgc gcgggtgccc cgggagaggg
     2341 tgactgggcg gcggcgccga aggcggccag cgcagcaatc accatggcgg cagccttgcg
     2401 ccacggatga gcaagcatga tttttcgcct ttgcggtttg tgtcaagcca ttatgggaag
     2461 ccggccacgg ggcacaatcc atgcattccc taaactctgc cgctacccgt gaccgccacc
     2521 cttgccttgc cggccagcct gtcccggctc tatatgcgcc ccggcttcct gctgcggcgc
     2581 gcgcaccaga tctccgccgc cgtgttcgag gatgaatgcc gcgaactggc actgaccccg
     2641 gcccagttcg gagtgctcac ggtgctgcag gtgcaccccg gcctcggcca gtcgagcctg
     2701 gcgcgcgcac tgggctacga caaggtgacg gtgctgcggg tgctgcgcgg gctggagacg
     2761 cggggcctcg tcacgcgggc gccggcggcg ggcaacaagc gcaacgtctc ggtggcactg
     2821 acggcgcgcg gcgcgtcggt gctggcgctg gcgcagaagc ccgccgaaaa agcttacaag
     2881 cggctgctgg cgccactgga caaggcgcag caggcgcaac tgctggcgtt gctgcaattg
     2941 ctgacgggcg agctggaaaa cgacgcgcgt gccgccttcg tcccgccagc cacccggtag
     3001 acccacttca ggtgttgccg ctgggtatgc tccagcgcgc cagcgcggca tcgtcgctga
     3061 cccgcgcgtc cacccagcgg ccgccctcgg gcgtgcgctc cttcttccag aacggagcct
     3121 gggtcttgag atagtccatc aggaattcgc aagcctcgaa gctctcgccg cggtgggccg
     3181 aggtcacggc caccatgacg atctggtcca gaggatgcaa caagccgacc ctgtggatca
     3241 cccgtgcggc gtagatgccg aaccggcgct gcgcctcgtc gatcatcgcc tcgatcgcct
     3301 tttcggtcat gcccgggtaa tgctcgagtt ccatcgaaac gacaggggcg gccgctgccg
     3361 ttgccccgtc gcccgcctgc ggaccttgcg cattccggtc gcgcacggtc ccgacgaaag
     3421 cgcagacggc ccccacgcgt ttgtcgccca ggcgcagcgc ggccacttcg tcggcgaggt
     3481 tgaaatcctg ggtctggatt gaaacgcggg aatgcatgct gtctattctg gcaccgggcg
     3541 catccgctga ggcgcaggcc cttgtgctgc ctcagccacc ggtgaccggg ggaaagaacg
     3601 cgacttcgca gccgtcgctc aatgccgcgg attcgttgct catcacctgg ttcagcgcca
     3661 cgcgcacggc cttgccgcgc gccaggctct gggcataggc gccgccgcgc gcgaccagct
     3721 cgtcgcgcaa tgcggccagg ctcgcggccg acgtatccac cgtttcgctg ccgtgcccca
     3781 gggcctcgcg gatcgaggcg aagtacttca cggtgagttt catgcgatca gctcggccag
     3841 ggagatgaag gatacggttt cgccccgggc gatcgcgcgg cccgcggggt tgtccacgag
     3901 gccgtcgctc cagactgccg aggtcagcac ggccgaactc tggttcggaa aaaggtccag
     3961 cccgcctgcc ggattgcgcc gcgcgcgcag gaactcgcgg cgtttgtcgg cgcgcggcaa
     4021 gctgaagtgc gcgggcaatg ggacagcggc cggcgccagc cgggtcgcgc cctgcagctt
     4081 gagcaggaaa ggccgcacca gcagcaggaa ggtcacgaaa ctggagaccg ggttgccggg
     4141 aagcccgatg aagtgcgtgg gcccgacctt tccataagca aacggcttgc ctggcttgat
     4201 ggcaatctgc cacaggtcga gcgaaccgag cgactggacg gccggcttga cgtggtcttc
     4261 ctcgccgacc gacacgcccc cgctggtgag aatgaggtgc tgatcgatgg aagcgcggcg
     4321 caaagcctcg acagtggcgt cgcgccggtc cggcacgatg cccaggtcgc tgacttcgca
     4381 gccaaggcgc tgcaagagcg cgcgcaggaa gaaccgattg gagttgtaga tcgcgccggg
     4441 tttcatctgc gccgtcggca cctcgccggg catcaccagc tcgtcgccgg tggaaaacag
     4501 cgccaccttc gggcgccgcg ccacgaccag cctgtccatc ccgataccgg ctgccagtcc
     4561 gagcgcggcg ggcgacatgc ggtctccctt ggccaacacg acggagccgc gcgcgacgtc
     4621 ttcgccgctg cggcggatcc actggcccgg ctggggcacc cgcaggatgc gcacgatctg
     4681 ctcgccggcc ggttcggtgt cttcctgtat cacgactgca tccgcgccgg cgggaatcgg
     4741 cgctccggtg aagatgcgcg cgacactgcc gggcgccaac ggcccgggcg cggcgccggc
     4801 cggaatgcgc tggctcaccg gcagcggcac gccttcatcg gcgatctcgt ggctgcgcac
     4861 ggcgtagcca tccatggaac tgttgtccag cggcggcacc tgcaattccg agacgaggtc
     4921 ctgcgccaac acccgtccgt cggcctcgaa ggtggacacc gtttcggtcc ccgccaacgg
     4981 atgcgcatgg cccagcaact cggccagcgc atcgtcgagt ggacgcagcg cggagcggcc
     5041 ggcgcccgcg gcggagggag cgttcatgcg tacatctccg ggtcgtagtc gaagcgggct
     5101 ccattgtccg tgagccactc gaccaccgcg tctgcgtcgt tcaaatccag caccgggcgc
     5161 agggtgggct ggggcaggcg gtcgggagaa tcggtggcga tggcgacgat gaagtcgtcc
     5221 tcgggataca gcgcctgctt gccgatggcg tcgcgccaga cctcgatctt gagcaagtcg
     5281 ctccccttga agccttcgac cagcacccaa tcgacgccct cgtagagttc ggcgatcagg
     5341 tgatgcgccg tcagcttcgc gggttgctcg aattcgcgga tcagggccag acgctggttg
     5401 gacgccacca cgacctcgaa ggcgccggcc tcacggtgcc gataggtgtc ctttccgggg
     5461 tggtcgatgt cgaacttgtg gtgcgcgtgc ttgaccaccg aaacgcgcag cccgcgcagc
     5521 ttgagcgcgg ggatgagccg ctcgaccagc gtcgtcttgc ccgacccgga gaaacccgcg
     5581 aaaccaacga ctttcatcgc gagctaggcg cagtgccgcg ccatgaactc cttgacggcc
     5641 tgcgcgtcgg ccggcatcac ggtgacgcgc cggggcagct gctcgatccc ctcgaaccgg
     5701 gccggacggt cgggctcacg gcccagggct tccacgatcg tctcggcaaa cttgatcggc
     5761 agcgccgtct ccagcacgat catcggcacc ccggcttgcg tgtgccggcg cgccaccttc
     5821 aggccgtcgg cggtgtgggt gtcgatgatc agcttgaagc gctggtaggt gtcgcggatg
     5881 gtcgcgacgc ggtccgcgtg ggtgctcttg ccgctgatga agccgtagcg tgcggatgcc
     5941 tgcctgaacg cggggtccgc gctcaggtcg aatcggcctt cccgcggcag cagttcgccg
     6001 aacagatgcc cgaccttggc cgcatcgcgg ccaagcaggt cgaagacgaa ccgttcgaag
     6061 ttgctcgcct tgctgatgtc catcgacggg ctcgacgtct cgtgcgtgtc ggcaccggac
     6121 cgcacgcggt acacgccggt gcggaagaac tcgtcgagca cgtcgttctc gttggtggcc
     6181 agcaccaggc gttcgatcgg caaccccatc atccgcgcca cgtggccggc gcagatgttg
     6241 ccgaaattgc ccgagggaac ggcgaagctg actttgtcgc cgttggagcg ggtcgcctgg
     6301 aagtagcccg cgaaataata gaccacctgc gccaggagcc gggcccagtt gatcgagttc
     6361 actgtgccga tgcgaaaccg gcgcttgaac tcgaggtcgt tggaaacggc cttgacgatg
     6421 tcctggcagt cgtcgaacac gccctcgatc gccaggttgt ggatgttggc gtcctgcagg
     6481 ctgaacatct gcgcctgctg gaacgggctc atgcggcctt cgggcgatgt catgaagacc
     6541 cgcacgccct tcttgccgcg catggcgtat tctgccgagc tgcccgtgtc gccggaggtc
     6601 gcccccagga tgttgagctc ttctccgcgg cgggccagct cgtactcgaa caggttgccc
     6661 agcagctgca tggccatgtc cttgaacgcc agcgtggggc cgttcgaaag cgactccagg
     6721 tacagcccgt cttccagctt cttcagcggc acgatcgcct cggtgccgaa cacgtcccgc
     6781 gtataggtct tgcggcacag cgcgcgcagg tgtgcagccg ggatgtcgtc gatgtagagc
     6841 gacaggatct cgaatgccag atcggcgtac ggcaggccgc gccacgcgga aagcgtcgca
     6901 tcgtcgacat ggggatagcg ctcgggcagg tagaggccgc catcgggagc caggccttcc
     6961 agcaggatct cgcagaaatg cttgcggtcg gcgtggccgc gggtggacat ataaagcatc
     7021 aggccagctc ttccttacgg atgcgcgtga tcggcgccag cacggtgggc aagccctgca
     7081 tctgcgcgat tacgtcgttc atcgtgcctt cgcgcacgct gtgggtcagg atgatgaggt
     7141 cggtctgttt ctcgccctcg ccggcttcgc gctgcagcat ggcgtcgatg ctgatgccgg
     7201 cggcggccag ctgtcccgtg actttcgcca gcacgccggc ctggtccgcc acccgaagcc
     7261 gcaggtaata gctggtgacg acctcgctca tcggcagcac cggcgtgtcg ctcatcgaat
     7321 cgggctggaa ggcaaggtgc ggcacacggt gcgcggcgtc cgcggtgtgc aggcgcgtga
     7381 tgtcaaccag gtcggcgatc acggcactgg ccgtcggctc gctgccggct cccttgccgt
     7441 agtacagggt ggtgcccacg gcatcggcct ggacgagcac ggcgttcatc gcgccctcga
     7501 cgttggcgat caggcgcttg gccggaacca acgcggggtg cacacgcagc tcgatccctt
     7561 tcagggcgcc cgtggccttg tcgaaactgc gcttcgtgat gcccagaagc ttgatgcggt
     7621 agcccagctg ctcggcgtag cggatgtcct gggcgcccag cttggtgatg ccctcgacgt
     7681 aagccttgtc gaactgcacc gggatgccga aggcgatggc cgacatgatc gtggccttgt
     7741 gggccgcgtc gacgccttcg atgtcgaaag tgggatcggt ttccgcgtag cccaggcgct
     7801 gtgcctcctt cagggcgaca tcgaaatcca gccccttgtc gcgcatctcg gagaggatga
     7861 aattggtggt gccgttgatg atgcccgcga tccattcgat gctgttggcc gtgaggcctt
     7921 cgcgcagggc cttgatgatc gggatgccgc ccgccaccgc cgcctcgaac gccaccatga
     7981 cgccccgtcg gtgggcggcg gcgaagatct cggtgccatg cacggccagc aacgccttgt
     8041 tggcggtgac cacgtgcttg cccgcctcga tcgcttccat gacaagctgc ctggcgatgc
     8101 cgtaaccgcc gatcagctcg acgacgatat cgatctcggg gttcgcgatc acggcacgcg
     8161 cgtcgtccac caccttcacg ccctcgccca cgaccgaacg cgcgcgtgcg gtgtccaggt
     8221 ccgccaccat cgtgatgtcg atgccgcgcc cggcgcggcg cctgatttct tcctggttgc
     8281 gctgcagcac attgaagacg ccgctgccta cggtgccgat gcccagcacg cctacttgga
     8341 tggctttcat ttggtggtac gtttcctgta gttttcgagg aacttcgcga tacggccgac
     8401 cgcctcgcgc aggtcgtcct catggggcag gaagacgatg cggaaatggt cgggcgtggc
     8461 ccagttgaag ccggtgccct gcaccagcat gacgcgcgtc tcctgcagca gttccaggaa
     8521 gaactgccgg tcgtcggtga tcggatagac cttgggatcg aggcgggcga acatgtagag
     8581 ggcggccgtg ggtttcacgc aggtcacgcc cggaatcgcg gtgatgagtt cataggccag
     8641 atcgcgctgg cggcgcaggc gcccgccttc gccaacgagc tcgttgatgc tctggtagcc
     8701 gcccagcgcg gtctggatcg cgtattgccc gggcacgttg gcgcacagcc gcatgttgga
     8761 gagcatgttc aggccctcga tgtagtcggc cgccggcttc ctgtcgccgg acacgatcat
     8821 ccagccggcg cggtagccgc aggaacggta gctcttggac agcgaattga aggtcagtgt
     8881 gagcacgtcc tccgacaggc tggcgatggc cgtatgtctt gcgccgtcgt acagcacctt
     8941 gtcgtacacc tcatcggcga agatcaccag gttgtgctcg cgcgcgatct gcacgatctc
     9001 cttgagcaga tcgtccgaat acaaggcgcc ggtcgggttg ttggggttga tgacgacgat
     9061 gcccttggtg cggggcgtga tcttgcgcct gatgtcctgc aggtcgggca tccagccatt
     9121 ggcttcctcg cacaggtaat gcaccggcgt gccgcccgac aggctggcgg cggccgtcca
     9181 cagcgggtaa tcgggcgccg gcagcagcag ttcgtcgcca tcgtccagca gcgcgttggt
     9241 ggccatggcg atcagctcgc tcgcgccatt gcccaggtag atgtcgtcca gggtgacgcc
     9301 cttgatgccc tgtttctggg attcgtgcat cacggccttg cgggccgcga agattccctt
     9361 gctgtcggag tagccggagg agttcggcag gttgcggatc atgtcctgct ggatttcctc
     9421 gggcgcatcg aagccgaaca cggcgaggtt tccgatgttc aggcggatga tcttgtggcc
     9481 ctcttcctcc atctgccgcg cggcgtccat gatcggaccg cggatgtcgt agaggacgtt
     9541 ggcgagcttg gctgacttct ggacggtttt caaaagcccc tccggaccgt tctgaaagtg
     9601 cgaaaactta taatttgacc acagttctgc cggttatcca ggcagcgcga caatgaactt
     9661 cgcccttacg cgcacccatg aaactgcaac ctgaccagtc cgacgtgcaa tcgatcagcg
     9721 gctacgggcc gggatgggtg ggtgtgaatg gggaaaaaat cacacgaagc gtgatcatca
     9781 cctccggtgg cgcgcgcctg gattgggcgg tcgatcgctt cgaggacttg ggcgcggacc
     9841 atttcgcgca gctggcgctg gtcgaggccg aggtcgtcat cttcggcagc ggctcgcgca
     9901 tccggtttcc cgatgccgcc tggctcaggc ccttgattgc gcggcgcatc ggcgtcgaaa
     9961 ccatggacac ggcggcggcg tgccgcacct ataacatcct ggcgcaggag gggcgcagcg
    10021 tcgcagtagc cctgctgctc gaacagccgg cctgacccac gccggtcgag aacgccgatt
    10081 cagggtaaaa tcagaggttg cgaggggaca acctagcccc cagtagcaac gagaaatcct
    10141 gtcacacgag aaacaaagtc ccatggcgat cgttgtcaac aaacccatcc ccgaatttga
    10201 agcaaacgcc acgggcggca tcaaagtcac caatacctcg cataacgggc aagtcctcgt
    10261 tctgtacttc taccccaagg acaacacgcc cggctgcacc accgaagcca tgcagttccg
    10321 cgacaaatac aaggacttcg tcaaggccgg cgccgcggtg ttcggcgttt cgcgcgacaa
    10381 catgaagtcg catgacgact tcaaggccaa gctcgagttg ccgttcgagc tgatcgccga
    10441 caccgaagag aagatgtgcc acatgttcgg tgtcgtgaag aacaagatca tgtacggcaa
    10501 gaaggtcaag ggcatcgagc gcagcacttt cctcatcggc gccgacggcg tcctgaagca
    10561 ggagtggcgt ggcctgaagg taccgggcca cgttgatgag gtgctcaagg cggtgaagct
    10621 gctgaagaaa gcggcttgac gttttgtagc aacatgcgcg gacccgcggt tgtagggcca
    10681 cgggacttgt gcataatgat ttcatgcagt tgattgcgac gcgccgcgtc atcaccgctg
    10741 acccagacaa agccgcccgg ctcgcccggc ggctttttcg ctttttgact tgccttttcg
    10801 ggaacctgct cctctatgcc tttgccccca gcccccacca agcgcgccgc cctgttgccc
    10861 ccggaagctt acgatgctcc ggctcgttcc tcgcacaagg cggctcgaaa atcggaggca
    10921 aacaccggca gcccgctggc tgaaaaaccg ctggtcctgg acttcgaccc gcgccagggc
    10981 ggcggcgagc tgcatcctga agcgttcaag gaccccaccg acggctattt cgatcatcag
    11041 gagagcgccg tccacaagag acggctcgct cccgagcccg cgccgcggcc ggcccgcaag
    11101 gtcaagccgg cggggccgag caagctgttc gtgctcgaca ccaacgtgct catgcacgac
    11161 cccatgagcc tgttccgctt cgaggagcac gacatcttcc tgccgatgat cgtgctggaa
    11221 gagctggacg gccacaagaa aggcatgacc gaggtggcac gcaatgcgcg ccaggccagc
    11281 cggtcgctgg atgcgctggc cgggacgcag ggcgccgaca tcgccagcgg cctgaagctg
    11341 gacaccaccg gccatcgcga ggccggcggc tgcctgttct tccagaccag gcaactcgac
    11401 agcacgctgc cgctggggct gccgcaaggc aaggcggaca accagatcct gggcgtggtc
    11461 caggccttgc gcgaacagca tgcgccgcgc gacgtcgtcc tggtgtccaa ggacatcaac
    11521 atgcgcgtca aggcacgcgc gctgggcctg gccaccgacg actaccagaa cgacaagacc
    11581 atcgacgacc tggagttgct gtactccggc gcgctggcgc tgccgtcgga cttctgggcc
    11641 aagcacggca agaccatcga gagctggcag agcggcctgc acacctacta ccgcatcacc
    11701 ggtccggtcg tcccgagtct gctgatcaac cagttcgtct atttcgagtc gcccggcgag
    11761 cccagcctgt atgcccgcgt cacggaaatc cgtgccaaga cggccgtgct caagacgctg
    11821 aaggactacg gccacctgaa gcactcggtg tggggcgtca cgacccgcaa ccgggagcag
    11881 aacttcgcga tgaacctgct gatggacccc gaggtcgatt tcgtcacgct gaccgggacg
    11941 gccggcacgg gcaagacgct catggccctg gcttccggct tgacccaggt gctggacgat
    12001 cgccgctaca ccgagatcat catgacccgc gccaccgtga gcatgggcga ggacataggc
    12061 ttcctgcccg gaaccgagga agaaaagatg ggtccctgga tgggtgcgct ggacgacaac
    12121 ctggaggtgc tggcgaaaac ggaaaccggc gccggcgaat ggggacgcgc cgccaccaac
    12181 gagctgatcc gcagccgcat caagatcaag agcatgaact tcatgcgcgg ccgcacgttc
    12241 ctgaacaagt acgtcatcat cgacgaggcg cagaacctca cgcccaagca gatgaagacg
    12301 ctgatcaccc gggccggccc cggcaccaag atcatctgca tgggcaacct ggcccagatc
    12361 gacacgccct acctgacgga gggctcgtcg ggcctgacct tcgcagtgga caagttcaag
    12421 ggctggcccc acagcggcca tatcatgctg gcgcgcggcg aacgttcgcg tctggcggac
    12481 tttgccagcg aagtgctgta acgcctgagc gggcgcccgc tcaggcgaac gcgatccgga
    12541 tgtcggacgg cacctgcagc gaccggtccg gcgcgcgcgc tggcttgacg gccgtcagct
    12601 cggcttccat ttcgaggatc acgttgcgct cgcggttgat cctgaccagg atctccgctg
    12661 ccgtcgcgtc cgggaagcgc gtgcgttcca ccttcagccg gatgacgccg gtcagccagt
    12721 cgccccggta gccgtagtcg gcgcgccagt aaaagggctg cgagaaatag ccgaaactgc
    12781 cgtggaagta ctggcgcacg tgggtcggat cctcgaaggc gtcatcgctt gcgccgtgcg
    12841 gaacacgcag caccatgcgc gcccccggcc tggcgatgcg gtgcagctcc tgcatcagcg
    12901 ccagcggatc gcggatgtgc tcaatcacgt gggacagcag aaactcgtcg aagctgtcgt
    12961 cgtcgaacgg cagcggcgac tcgcggcaat tgtccaggtc ggcgacgatg tcgacgccgg
    13021 gcagcgccgc aatgtccagg ttgacccaac cgtccaggat gttgcggccg cagccgacgt
    13081 tcaggcgcag cggcagtccg gcaggcgcta ggctttcctt gcctgcggtc tgggctgggg
    13141 acgccatcag gctggagaag agcgccttga gcatcaccgc cgtcagaagt cgctgctgcc
    13201 cgaagccagg ctctcgaact tcgtcagcgg cttcacgaac gccagcttga cggtcccggt
    13261 cgggccattg cgctgcttgc tgatgatgac ctcggcgacg cccggctcct tggagtcctt
    13321 gttgtagtag tcgtcgcggt agatgaacat gatcacgtcc gcgtcctgct cgatcgctcc
    13381 cgattcgcgc aagtcgctca tcatcggccg cttgtccgtg cgcgattcca cgccgcgcga
    13441 cagctgcgac agcgccatca cggggcactg gagttccttg gccagcatct tgaggccgcg
    13501 cgagatttcg ccgaccgcgg tggcccggtt ttcttcgctc atgctgctgg acacgctcat
    13561 gagctgcagg tagtccacca cgatcaggcc aagcttgccg ccgcattgcc gcgccagccg
    13621 gcgagcgttg gctcgcaact cgctgacagt gaggcccggc gtctcgtcga tgtgcagcga
    13681 cacgttgcgc agcttctcga tcgcctcgct caggcgcggc cattcgtcgt cggtaagctt
    13741 gccggtgcgc aggtgcatct ggtcgatccg gccgatcgag cctacgattc gcacggccag
    13801 ctggctcgcg cccatttcca tggaaaacac cgccaccggc aattcttcat gcagcgccac
    13861 gtgctcggcg atattgatcg caagggccgt tttgcccata ctcggccgcg cggccagcac
    13921 gatcatgtcg ccggcctgga agcccgaggt aagccggtcc aggtcgtaga agccggtcgg
    13981 cacgcccgtg atgtcgttcg ggttgtccgc catctcctgc acgcgatcca gcaattggac
    14041 caccaggctg tccatggcct ggaagccctg cttcatgcgc gagccttcct cgccgatatt
    14101 gaagatcttc tgctcggcct cgtccaggat cttgtcgacc ggcttgccct gcgtattgaa
    14161 cgccgcggtg gcgatctcgt cgctggcgct caccagcttg cgcaggatgg agcgctcgcg
    14221 cacgatttcg gcatagcgcc ggatgttgct cgcgctgggc acgtattgcg ccagcgagtt
    14281 gaggtaggcc agcccgccga cctcgtccgc cttgcccagc ccctgcagct gctcatggac
    14341 cgtgatcacg tcggccggct tggtggcgtt gatcaggccg ccgatcgccg cgtagatcag
    14401 ccggtgctcg tagcgataga agtcgccgtc ggtgagcagg tcgcccatcc ggtcccaggc
    14461 accgttgtcc agcaacagcc cccccagtac gctggactcg gcttcgatgg agtgcggcgg
    14521 gatgcgcagc tgggcgatct ggtgatcgcg ggaaaagtcg tcgtcgacgg aggtgagtac
    14581 ggcggacatg ggctctcttt ggaaccgcac aatgctaagg cgaggctgtg aaaacgtcca
    14641 gtaataacac tgggtacagc ctgtggacgg atccggaaat cctgtggacg gccggtggag
    14701 caacagacaa aaaaggccgc ccgaaggcgg ccttgaactg gtaatagaag aacgcggcct
    14761 tttttcttct gttgtcgttt ttacgcggtc tcgccgtaca ccgtcacggt gatgtccacc
    14821 gccacgtccg tgtgcaacgc gacgctgaca gtgctgtcgc cggtatgttt gatcgggccg
    14881 gtgggcatgc gcacctgcgc cttcgccact ttgtagccca gcttgttcag ttcctcggcg
    14941 atatcggaat tggtgactga gccgaacagg cggccgtcga cgcccgcctt ttgggtcagc
    15001 ttcacggtcg tgccggcaag cttctcggcc tcggactgcg actgcgccag cttggccgct
    15061 gcggcttttt cgagttcgac gcgcctggcc tcgaattcgg ccttgttggc ttcggtggcc
    15121 cggcgggcac ggccctgcgg aatcaggaaa ttgcgggcgt agccgtcctt gaccttgacg
    15181 atctcgccga gatttccaag gttgacgacc ttgtcgagca gaatgacttg catgttcttc
    15241 gccccttaga tgcggtgctg gtcggtgtaa gggaccaggg ccaggaagcg cgcgcgcttg
    15301 atggcggtat tgagctggcg ctggtagatc gcccgggtgc cggtcaggcg ggccggaatg
    15361 atcttgccgt tttccgcgat gaagtcgcgc agcgtgtcga cgtccttgta gtcgatttcc
    15421 tcgacgtggg cgacggtaaa gcggcagaag cgcttgcgct tgaacagcag cgattgggtg
    15481 ttgcgcttcg gacgcttgtc cttgttgaac ttcttgaacg tggccatagg ggactttcta
    15541 aagtgttgag gacggagctg aagatctgtc ccgcaaagtt ttcaatgtgt tgggacagag
    15601 ctgatccgtc caaaatatct aaatctcttg aaactcctgg atgtggagta ccgggtgctt
    15661 gccgtttcgg ggcgtcgcca ggaatccggt gaacttccag ctgctgccga tggcctgctt
    15721 gcccagcgtt tccgccaagc cgccaaatgc caccgccctg atcgcaaccg caacctgcct
    15781 cggctgccct gcttcggtga cctcggactc gtgttcgagc ctgaaatcca gggccggcaa
    15841 tccggctggg gtataccgca ggacaccggc ctcggcgata cgggcactca agacgagctg
    15901 gttcactccc ggcaaagcgg cctcaggcgg cggcctggta ctccgcctgc tgcgccttgc
    15961 gggcctcttc gcgctccacg gtcctcatca tcgcggacgg gcccgtgtcg gccttcttgc
    16021 gctgcaccgt gaggtggcgc agcacggcgt cgttgaagcg gaaggcatgc tccagctcgg
    16081 ccatcaccgg ctggtcggcc tcgatgttga tgcacaggta atgagccttg gcgagcttgt
    16141 tgatctggta ggccagctgc cggcggcccc agtcttccac acggtgcact ttgccgccgc
    16201 cggcggtaat catgcccttg tagcgttcca gcatggccgg aacctgctcg ctctgatccg
    16261 gatggatcag caaaatgatc tcgtaatgac gcataggact ccttgaggat gatgttgcag
    16321 ccacccactg cgtcggtagc ggtgtggcaa ggaaagcctt cgattataga ggcaaagaaa
    16381 taaccgcccg ggcccgaagg ccgaggcggc ttgagcaaga gcgcgccagc ccctgcgggc
    16441 cggcgccttt gctaccggct cacaaaccgt gcaccaggct cttggcggac cccttcaggc
    16501 tggggtagcg aacgtgctcc accaggtcct gaatccgtgc gttcggcgcc ggggtgaaca
    16561 ggctgaccag gatgatcacc gcaaatccga gcggcacgcc gaacaggccg gccgagatcg
    16621 gcaggatgcc ccaccacagc tcgatcggcg aagtgacgcc gaacacgctg cgcatccagg
    16681 gctgggtgat caccatgtag tagaaggtga tgcccaggcc cgaggccatc cccagcgaag
    16741 ccgctatacc ggtcgcccgt ttccagaaga ttcccagcac cagggccggg aagaaggcgg
    16801 ctgccgcgaa cgagaaagcc gccgagacca ggaacaggat gtccgccggc ttctgcgatg
    16861 ccacccccgc ggccgcaagg gccaccacca gcagcagtat cttcgacagc gtcacccgcc
    16921 gtgccgtgga ggcgttcggg tcgatcatct tgtagtacag gtcgtggctg agcgcattgg
    16981 caatcgtcag cagcaggccg tccgcggtgg acagcgccgc tgccaggccg ccggcggcca
    17041 ccatcccgga caccacatac ggcaagccac cgatttcagg cgtcgccaac acgatgatgt
    17101 cgccgccgat cttcatttcg gtcaattgga agatcccgtc cttgttgacg tcgatcaccg
    17161 acagcaggcc cgggtcgact cgctgccagt tgttgatcca ggccggcaac ttgtcgaacg
    17221 gcgttcccac cagcacgttg aacacctcgt acttgaccag caccgcgaga gcaggcgccg
    17281 tgaagtacag caggaagatg aagaacagcg accaggtcac cgaatcgcgt gcctgtttga
    17341 cacttggcgt ggtgtagtag cgcatcagga tgtgcggcaa cgcggccgtg cccaccatca
    17401 ggcagaagat cagcgccagg aagtttcgcc gcgactcgtc gaaggccttc tgctcagccg
    17461 gggtgccctt tggatcaccc gcaaaaacct gggcgtgatt gggcatgccg gccagcggtt
    17521 tggcgcgatc caggttagca gccttggcct tggtccaagc gtccttggcg gcggcttcgt
    17581 ccttgggcac ggccgccagc tccttctccg cggccgtcac ttgcgaggcc atcgcgttgg
    17641 cggctttgag gtccgccagt ttcttctcgg cggccgccct gtccgtggcc agtgccgtcg
    17701 ggatgttctt cagcttcgca tcgaactcat cggcacgggc cttgaagatc gccacgacct
    17761 cgagttcttt cgggtccgtg cgaaggtcgt cgccgcgctt ggacactttc tcgagctggt
    17821 agccgtagat cgcttggggg atcggcacgc cggtttgctt caccgccagc cacaccacag
    17881 gaatcatgta ggccatgatc aagatgatgt actgcgccac ctgggtccag gtcacggcgc
    17941 gcataccgcc caggaacgag cagaccagga taccgcccag gcccaggaaa atccccagct
    18001 cgaatgcgac gccggtcagc cgcgtcgtga tgatgcccac gccat
//
LOCUS       DASWIY010000211         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1989900, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000211 DASWIY010000000
VERSION     DASWIY010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989900"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..988
                     /gene="aat"
                     /locus_tag="VGA59_07755"
     CDS             242..988
                     /gene="aat"
                     /locus_tag="VGA59_07755"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX9719613.1"
                     /translation="MITWLRDEHDALPDPCLALPRGSDAPGLLAAGGELTPARLTEAY
                     SKGVFPWYSEGQPILWWSPDPRMVLLVDEFKLSRSLRKTLTRFVRTPGCEVRIDRVFD
                     RVITACAATPREGQDGTWILPEMVDAYRHWHDLGAVHSFETWVAGELTGGLYGVSLGR
                     MFFGESMFSHRSDASKIALAALVCFCREHGIAMIDCQQRTPHLASLGGRELPRAEFER
                     RLTPCLREPPVGDWTYHHRHWGHLLKLQNE"
     gene            1013..>1553
                     /locus_tag="VGA59_07760"
     CDS             1013..>1553
                     /locus_tag="VGA59_07760"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX9719614.1"
                     /translation="MTHPKELPLASLQFYATAPYPCSYLEGRMARSQVATPSHLIHAD
                     AYSGLVANGFRRSGMFTYRPYCDGCRACVPLRVPVATFAPTRSQRRAWKAHQNLQARV
                     LRLCFVPEHYQLYLRYQAGRHSGGGMDHDSIDQYTQFLLQSRVNSRLVEFREPAAGGL
                     GTLKMVSILDILNDGLSAVY"
BASE COUNT          249 a          560 c          499 g          245 t
ORIGIN      
        1 cgaaagtcgg gatccactgt gtgcgcccta tggatcccgg ccttcgccgg gatgacgaac
       61 cgacgtggtg gggcaaaggt gtgcgaccgg ttggggccga aaagcgacac acaccggctc
      121 ccgccgtttc atgcgtcgcg agtcacgcca agcgcggtgg agcaactgag atgacaaccc
      181 ccacctcgct tcactcctgc cccccgaggc ggcgctcaac cgccgagcca tgcgtcaacc
      241 aatgatcacc tggctacgcg acgagcacga cgcgctgccc gacccctgcc tcgcactgcc
      301 gcggggatcg gacgcgccgg ggctgctggc ggccggcggc gaactgacgc cagcccggct
      361 gaccgaggcc tattccaagg gcgtgttccc ctggtacagc gagggccagc ccatcctgtg
      421 gtggtcgccc gacccgcgca tggtgctgct ggtcgatgag ttcaagctgt cgcgttcgct
      481 gcgcaaaaca ctcacccgct tcgtgcgaac gcccggctgc gaggtgcgca tcgacagggt
      541 cttcgatcgc gtgatcaccg cctgcgcggc cacgccccgc gaaggtcagg acggcacctg
      601 gatcctgccg gagatggtgg acgcctaccg gcactggcac gacctgggcg ccgtgcacag
      661 cttcgagacc tgggtggccg gtgagctgac cggcggcctg tacggcgtga gcctcgggcg
      721 gatgttcttc ggcgagtcga tgttctcgca ccgcagcgac gcatcgaaga tcgcgctggc
      781 cgcgctggtg tgcttttgcc gcgagcacgg cattgcgatg atcgactgcc agcaacgcac
      841 gccgcacctc gcgtccttgg gcggccggga gctgccgcga gccgaatttg agcggcgact
      901 caccccttgc ttgagggaac ccccggtagg cgattggacc tatcatcacc gccactgggg
      961 gcatctgctg aagcttcaga atgagtgacc ccgcgccgaa ggaccccggc ccgtgaccca
     1021 ccccaaagag cttccgctcg catcactgca gttctacgcc acggccccct acccgtgcag
     1081 ctacctggag ggccgcatgg cgcgctcgca ggtggccacg cccagccacc tcatccacgc
     1141 cgacgcctac tcgggcctgg tggccaatgg cttccggcgc agcggcatgt tcacctaccg
     1201 gccctattgc gacggctgcc gcgcctgcgt gccgctgcgc gtgccggtgg ccacctttgc
     1261 gcccacgcgc agccagcgcc gcgcctggaa ggcgcaccag aacctgcaag cgcgcgtgct
     1321 gcggctttgt ttcgtgcccg agcactacca gctctacctg cgctaccagg ccggccgcca
     1381 cagcggcggc ggcatggacc acgacagcat cgaccagtac acccagttcc tgctgcaaag
     1441 ccgcgtcaac tcgcggctgg tggagttccg cgaaccggcc gccggtgggc tgggcacgct
     1501 gaagatggtg tccatcctgg acatcctcaa cgacgggctt tcggccgtct aca
//
LOCUS       DASWIY010000212         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3097254, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000212 DASWIY010000000
VERSION     DASWIY010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3097254"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VGA59_07765"
     CDS             <1..274
                     /locus_tag="VGA59_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9719615.1"
                     /translation="GLHAALRVGRLFDEGRAAPEMVSLIKRNNCGKALDIARVARDMH
                     GGNGIADEFHVIRHVMNLEAVNTYEGTHDVHALILGRAQTGIQAFF"
     gene            337..1614
                     /locus_tag="VGA59_07770"
     CDS             337..1614
                     /locus_tag="VGA59_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX9719616.1"
                     /translation="MSLSLETRFERYCDRIVDTLRHADREVPARWYIKGLMLPGERKS
                     VEPMAARVQPDNVRSAHQSMHHLVAEAAWSDAAMLSAVAEAVLPKLVCDDAPVHWIVD
                     DTGFPKKGKHSVGVARQYCGQTGKQDNCRVAVSLSIATARGSLPVGYRLYLPEEWADD
                     PERRAKVGVPEELGFETKPALAMRQIEAALAAGYPRGVVLADAAYGDETAWREQLAGH
                     GLSYAVGVRPGTTVWWGKYQPLAGKPGDRVGRPRQRLQRDAAHQPVSVGQLARALPAK
                     TWRTVTWRQGVSGPLSSRFARVRVRAAHRDRPRDEEWLLIEWPEEAAEPAHYWFSNLP
                     KNRPWQAMIDTVMGRWRIERDYEELKQELGLGHYEGRNWRGFHHHASLCIAAYGFLML
                     ERLDGSKKNSTRFKAPPLPIGFRPRGAGADAAP"
     gene            complement(1731..>2093)
                     /locus_tag="VGA59_07775"
     CDS             complement(1731..>2093)
                     /locus_tag="VGA59_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9719617.1"
                     /translation="LSDGELVRLSKDDFMKLLNEPLLNWVNYRDAKTMVENKGAQWLD
                     VRLPTEYEERHFEGSVNVPFFSLRMRLKQLDRATPYIVCCDTGSRSSAAAFLLNERGY
                     DAYVLRGGLNATPAGTFP"
BASE COUNT          363 a          654 c          736 g          340 t
ORIGIN      
        1 cggcctccac gcggcgctgc gggtcggccg gctgttcgac gaggggcggg cggcgccgga
       61 aatggtcagc ctgatcaagc gcaacaactg cggcaaggcg ctggacatcg cccgcgtcgc
      121 ccgcgacatg cacggcggca acggcatcgc cgacgagttc cacgtcatcc gccacgtcat
      181 gaacctcgag gcggtcaaca cctacgaggg cacccacgac gtccacgccc tcatcctcgg
      241 tcgcgcgcag accgggatcc aggccttctt ctagtaccac tgtgttacaa aataaggcat
      301 tatggcagcc tctccaagca ctttgtgagg tgcgccatga gtctgagcct ggaaactcgg
      361 ttcgagaggt actgtgaccg gatcgtggac acgctgaggc acgcagatcg tgaggtgccg
      421 gcgcgctggt acatcaaggg cctgatgctg cccggtgagc gcaagagcgt ggagccgatg
      481 gcggcccgcg tgcagccgga caacgtgcgt tcggcccatc agtccatgca ccacctggtg
      541 gcggaagccg catggagcga cgccgcgatg ctgtcggcgg tggcggaggc cgtgctgccc
      601 aagctggtgt gcgacgacgc accggtgcac tggatcgtgg acgacaccgg gtttccgaag
      661 aagggcaaac actcggtcgg cgtggcgcgc cagtactgcg gtcagacggg caagcaggac
      721 aactgccgag tggcggtgtc gctgtcgatt gccacggcgc ggggcagcct tccggtgggc
      781 tatcgcttgt acttgccgga ggagtgggcc gatgatcccg agcgccgcgc gaaggtcgga
      841 gttcccgaag agctcggctt cgagaccaag cccgcgttgg cgatgcgcca gatcgaagcg
      901 gccttggcgg cggggtatcc gcgcggtgtg gtgctggccg atgccgccta cggggacgag
      961 accgcgtggc gcgagcaact tgccgggcac gggttgtcgt atgccgtggg ggtgcggcca
     1021 ggaacgaccg tctggtgggg gaaataccaa cccttggccg ggaagcctgg ggaccgcgtc
     1081 ggcagaccgc gccagcggct ccagcgcgac gcggcgcacc agccggtttc cgtggggcag
     1141 ttggcccgag cactgccggc gaagacgtgg cgtacggtga cgtggcgcca aggcgtgtcg
     1201 gggccgctct cctcgcgctt tgcccgggtg cgggtgcgcg ccgcccatcg cgaccggccg
     1261 cgggacgaag aatggctgct catcgagtgg cccgaggagg ccgctgaacc ggctcactat
     1321 tggttcagca acctcccgaa gaaccggccg tggcaggcga tgatcgacac ggtcatgggg
     1381 cgctggcgca tcgagcgcga ctacgaggaa ctcaagcagg agctggggct cggtcactac
     1441 gaaggccgca attggcgagg cttccatcat catgcgagtc tgtgcattgc cgcctacggg
     1501 ttcctgatgc tggagcgcct cgacggcagt aaaaaaaact ccactcgatt caaagcgcct
     1561 cccttaccca tcggcttccg cccgcgcggg gctggggccg atgcagcgcc atgaaccgac
     1621 gtcgatcgcc acgtgccgct tccgtctggc acgcgctatc gccagaacgc tgctgcagtg
     1681 tccttgttgc ggctacagga gagtgagggc ttattactaa cacagtagta ttaggggaag
     1741 gtgccggccg gggtggcgtt gagccccccg cgcagcacgt aggcgtcgta accgcgctcg
     1801 ttgagcagga aggccgcggc cgaactgcgg ctgccggtat cgcagcagac gatgtagggg
     1861 gtggcgcggt cgagctgctt caggcgcata cgcagcgaaa agaacggcac gttgacgctg
     1921 ccctcgaagt ggcgctcctc gtactcggtc ggcaggcgca catcgagcca ctgcgccccc
     1981 ttgttctcga ccatcgtctt ggcatcgcgg taattgaccc agttcagcag cggctcgttg
     2041 agcagcttca tgaagtcgtc cttggacagg cgcaccagct cgccgtcgct gag
//
LOCUS       DASWIY010000213        12035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2362219, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000213 DASWIY010000000
VERSION     DASWIY010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 12035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12035
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2362219"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VGA59_07780"
     CDS             <1..247
                     /locus_tag="VGA59_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9719618.1"
                     /translation="EQGITVEIGNWRGVYGAPGIAPAQRKALTDMVLAALKTKSWAES
                     LEKNNWTPAVMSGPAFEKFVDDEFASLRATMVKSGMV"
     gene            319..834
                     /locus_tag="VGA59_07785"
     CDS             319..834
                     /locus_tag="VGA59_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX9719619.1"
                     /translation="MFSRTNPALAQTLVGAGVSLIGLALGAGAVTIPSAAGYGGVGPN
                     FLPWLVAVVLVACGVLMVREARSGGFRQLDPPSGAANAWWPGVVWVSAGMLANAALIT
                     TIGFILSCALCYLLAVQGMRRASAQAGAGAPGTLLADAAVGLAISAPVFWTFTKFLAI
                     NLPGLTNTGWI"
     gene            836..2344
                     /locus_tag="VGA59_07790"
     CDS             836..2344
                     /locus_tag="VGA59_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX9719620.1"
                     /translation="MEIFNSLMQGFGTALTFSNLMWCFAGSVLGTAVGVLPGIGPAVA
                     VAMLLPITAKVDITASMIFFAGIYYGAMYGGSTTSILLNTPGETASMVTAMEGNRMAK
                     SGRAGAALATSAIGSFVAGTIGTVIVTMFAPTVAEFAVKLGPPEYFLLMVLAFTTVSA
                     VLGKSTLRGMTALFAGLALGLIGLDQISAQARYTGGIPELLDGIEIVLVAVGLFAVAE
                     VLYFALYEGRVVETRNRMSRVYMSARDWKRSVPAWLRATAIGSPFGCIPAGGTEIPTF
                     LSYAVEKKLAKGEDLKEFGTTGAIEGVAGPEAANNATVTTAMIPLLTLGIPISSTTAI
                     LLGAFQNYGIQPGPQLFTSSAALVWALIASLYIGNVMLLVLNLPMVGLWVKLLKIPKP
                     QLYAGILIFATVGAYGMRQSAFDLVLLWAIGIVGVLMRRFDFPTAPVVVGMILGPLAE
                     AQLRNAVSIGEGSAMVFLQRPMSAVLVVLIVAVLLLPRLYKLMQRRRPAPAA"
     gene            2371..3867
                     /locus_tag="VGA59_07795"
     CDS             2371..3867
                     /locus_tag="VGA59_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX9719621.1"
                     /translation="MQETDSLPRDGQSILELAARSMFHLFSSISQGMFLVDRTGRIVW
                     LNEGYKRFLPALGFSSVDQVVGHMVEDVVPNTQMRRVLETGEPILIDLLTNRAGTFVV
                     SRIPLRDDQSGELIGAIGIVLFDHPETTLQPLISKFARLQRDLDDARRELASQRRTKY
                     TLASFVGTSPATVEVKRQARRAALSSSPVLLLGETGTGKELLAHAIHAASARSAGPLV
                     SINIAAVPDTLLEAEFFGVAPGAYTGADRKGRVGKFKLADGGTLFLDEIGDMPPSLQP
                     KLLRALQEGEIEQLGSNKVVSFDARVIAATSRDLAALVREGKFREDLYYRLNVLPIRV
                     PPLRERRPDIPALVEVLAEDIALRGGSAPLELGADAIALLSAQDWRGNIRELRNVMEQ
                     AAMRSDSQRIEVQHLAQVLRESGVKNIAAPAVASEPIPPSSQVLRPLAEQVAELERRA
                     IDAALTATGGNKLAAAKLLGISRAKLYERLDGVTDFQTVSVNKTIRIA"
     gene            3987..5177
                     /locus_tag="VGA59_07800"
     CDS             3987..5177
                     /locus_tag="VGA59_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9719622.1"
                     /translation="MQRRTWVALAALAASAFTLPVAFGQAKDIKIAHIYSRTGPLEAY
                     GKQTATGLMMGLDYATNGTMSVNGRKITVIEKDDQGKPDLGKSLLATAYSDDKVDLAV
                     GPTSSGVALAMLPVAEEYKKILLVEPAVADSITGDKWNKYIFRTGRNSSQDAISNAVA
                     LDKPGVTIATLAQDYAFGRDGVKAFKESVKKAKFVHEEYLPANTTDFTAGAQRLIDKL
                     KDQPGRKVIWVIWAGAGNPFKIADLDLKRYGIEIATGGNILPAMGAYKQFPGMEGATY
                     YYFGIPKNPVNEALVASHYKQFKSPPDFFTAGGFAAAMAVVTALKKTNGDTGTDKLIS
                     AMEGMSFDTPKGKMTFRKEDHQAMQSMYHFKIKSDPAFAWGVPELVREIKPEEMKVPI
                     RNKR"
     gene            5423..6238
                     /locus_tag="VGA59_07805"
     CDS             5423..6238
                     /locus_tag="VGA59_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9719623.1"
                     /translation="MSLLATKDLTIRFGGHVAVNAVTCAFQPGTLTAIVGPNGAGKTT
                     YFNLISGQLRATAGQVTLDGRDLSGMSASQRTHAGLGRAFQLTNLFPRLTVLENVRLA
                     VQAARAGKHRRGFNLWSIWSDHRELTARAHEILRAVALAPKEHELVAGLPHGDQRKLE
                     VALLMGLESRVFMFDEPTAGMNAAEVPVILNLIRKLKEDASKTILLVEHKMDVVRELA
                     DRIIVLHNGALVADGDPAAVIASPIVQEAYLGVAPTVRAGPVEAVPGASREAA"
     gene            6235..6960
                     /locus_tag="VGA59_07810"
     CDS             6235..6960
                     /locus_tag="VGA59_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9719624.1"
                     /translation="MNENMLSLQGVHTHIGAYHILHGVDLTVPRGKLTMLLGRNGAGK
                     TTTLRTIMGLWHASQGRITFNGQDITQLATPQIASMNVAYVPENMGIFSDLSVKENML
                     LAARWARNAGQIDAERLKWIFTLFPAVQTFWNHPAGKLSGGQKQMLAVARAIVEPREL
                     LIIDEPSKGLAPAIINNMIDAFGQLKASGVTILLVEQNFNFAKRLGDTVAVMDNGVIV
                     HAGSMKELAEDEALQHSLLGLAI"
     gene            6957..7937
                     /locus_tag="VGA59_07815"
     CDS             6957..7937
                     /locus_tag="VGA59_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9719625.1"
                     /translation="MNKIDFDWKPLALVPVLALLVLPFIGSGSTWVTLTVAGLAMGMI
                     IFIIASGLTLVFGLMDVLNFGHGVFIALGAFVATSVLGAMGDYTASASLWRNMMAVLP
                     AMVVAMLVAGAVGVAFERFIVRPVYGNHLKQILITMGGMIIGEELIKVIWGPLQIPLP
                     LPEGMRGSIFLGDAGVEKYRVIAVVVGLLVFGILLWVLTRTKIGLLIRAGVQDREMVE
                     SLGYRIKVLFVGVFVVGSALAGLGGVMWGLYQQSVIPQMGAQVNILIFIVLIIGGLGS
                     TTGALIGALLVGLMANYTGFLVPKAALFSNIALMMAILLWRPQGVYALSR"
     gene            7949..9016
                     /locus_tag="VGA59_07820"
     CDS             7949..9016
                     /locus_tag="VGA59_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9719626.1"
                     /translation="MLNRLISNDYPRSRVLAVLLVAILLALAFTPFIVPGVKALNVAA
                     KILVFIVLVASFDLLLGYTGIVSFAHTMFFGIGAYGIAIASTRMGPGWSAILAGLAVA
                     LALSFVLALAVGLFSLRVRAIFFSMITLAVAAAFQTLASQLSEITGGEDGLTFKMPEL
                     LSPGFQLSESPFLGVSLDGRLISYYLLFFTTLVLLLAMLRIVNSPFGRVLQAIRENEF
                     RAEAIGYRVVLYRTTSSILSALFATVAGAMLAIWLRYNGPDTSLSFEIMLDVLLIVVI
                     GGMGTIYGAAIGSGVFVVAQSYLQDLLGLGSDATSGLPWLSALLSPDRWLLWLGLLFV
                     LSVYYFPTGVVGRLRAMGARG"
     gene            9070..9987
                     /locus_tag="VGA59_07825"
     CDS             9070..9987
                     /locus_tag="VGA59_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9719627.1"
                     /translation="MQSSSCYATCAGREIHYTEWGAQHRRTVIAWHGLARTGRDMDEL
                     AQHLSSRFRVICPDTLGRGLSQWSPDPGNEYTLAFYARLAADLLEQLDIGEAHWVGTS
                     MGGSIGTVCAAGLLQPQLKGRIRSLVLNDNAPHLAEPAVARIRAYAGNPPAFETIAEL
                     EAFFRQVYKPFGWLSDAQWRRLTETSTRRLPDGRVTPHYDPAMVRQFIDHPRDYELWD
                     HYDAIDIPVLCLRGAESDLVLRETTDEMLRRGPGSRGLTRVVEVAGCGHAPALNVPEQ
                     LELVASFIEQRDDAVAPAAPGTTGPLRWP"
     gene            complement(9962..11065)
                     /gene="hemH"
                     /locus_tag="VGA59_07830"
     CDS             complement(9962..11065)
                     /gene="hemH"
                     /locus_tag="VGA59_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX9719628.1"
                     /translation="MAASPSFQQEPEFSHGRAPGIAVLYCNLGTPDEPTPGAVRRYLL
                     EFLADPRVVEIPRLLWWPLLHGLILPFRSGKSAAKYAKIWTADGSPLLLWTDKQAKLL
                     QGWLGEHGHHVKVRFAMRYGNPSIASQLDALKAGGATRILVLPAYPQYSGTSTASLVD
                     AVAAWSTGIRSVPELRFVNRYHDDRGYIQALARRIEGYWREHGRGEHLVMSFHGVPER
                     TLRLGDPYHCECQKTARLLASHLGLEKERYTLTFQSRFGKAKWLEPYTEPTLRALARR
                     GIGRVDVVCPGFTGDCLETLEEIAIEGRQAYLTSGGKEFHYIACLNDDRGWIAALGCV
                     AERHLAGWPTRETPDADVLRESREAAMASGAAR"
     gene            11162..11854
                     /locus_tag="VGA59_07835"
     CDS             11162..11854
                     /locus_tag="VGA59_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX9719629.1"
                     /translation="MLDVSKIKAISIDLDDTLWPIWPTIERAEKVLHDWMVEYAPMAA
                     ALFSSPSALRDIRNHMAASRPDLKNDLSAVRRESIRLALYRAGENPLLADQAFEVFFA
                     ERQRVTLFDDVQPSLQFLSARFPLVSVSNGNADLERVGLGSYFRAAVSAREFGVGKPD
                     PRIFHAAAGAVDLTTEHVLHVGDDATLDALGALNAGMQAAWVNRLDHLWPHELEPHVK
                     LTNLTELCALFK"
BASE COUNT         1991 a         3925 c         4005 g         2114 t
ORIGIN      
        1 agagcagggc atcaccgtgg agatcgggaa ctggcgcggc gtgtacggcg cgcccggcat
       61 cgcccctgcc cagcgcaagg ccctgaccga catggtgctg gcggcgctca agaccaagtc
      121 gtgggccgag tcgctggaga agaacaactg gacgccggcc gtgatgagcg gcccggcgtt
      181 cgagaagttc gtcgacgacg agttcgccag cctgcgtgcc acgatggtga agtccggcat
      241 ggtgtgaccg gccgcgccgg gaagaactcc gcccggttcg ccgggctttt ttcttgcagg
      301 ttgctcaggg atctatccat gttttcacga accaaccccg cgctcgcaca gaccctggtg
      361 ggcgccggtg tctcactcat cgggctggcc ctgggcgccg gcgcggtcac gatcccctcc
      421 gcggccggat acggcggcgt cgggccgaac ttcctgccct ggctggtggc cgtggtgctg
      481 gttgcgtgcg gtgtcctcat ggtgcgggag gcgcgcagcg gcggttttcg gcaactggat
      541 ccgccatccg gggcggcgaa cgcctggtgg ccgggggtgg tctgggtgtc cgccgggatg
      601 ctggccaatg cggcgctgat cacgacgata ggctttatcc tgagttgtgc gctgtgctac
      661 ctgctggcgg tgcagggcat gcgccgcgcg agcgcccagg ccggcgctgg tgcgccgggc
      721 accctgttgg cggacgcggc agtgggcctg gcgatatctg cgcccgtgtt ctggacgttc
      781 accaagttcc tcgcaatcaa tctgcccggc ctcacaaaca ccggctggat ctgacatgga
      841 gatcttcaac tccctgatgc agggcttcgg cacggcgctc acgttcagca acctgatgtg
      901 gtgcttcgcg ggttccgtgc tcggcaccgc cgtcggcgtg ctgccgggca tcggccccgc
      961 ggtggcggtg gcgatgctgc tgccgatcac ggcgaaggtg gacatcacgg cctcgatgat
     1021 cttcttcgcc ggcatctact acggcgcgat gtacggtggc tcgaccactt ccatcctgct
     1081 gaacaccccc ggggaaacgg ccagcatggt cacggccatg gagggcaaca ggatggccaa
     1141 gagcgggcgc gccggcgcgg cgctggccac gtccgccatc ggctcgttcg tggccggcac
     1201 catcggcacg gtgatcgtca cgatgttcgc gccgacggtg gccgagttcg cggtcaagct
     1261 cggcccgccc gagtatttcc tgctgatggt gctggccttc accaccgtga gcgcggtgct
     1321 ggggaaaagc accttgcgcg gaatgacggc gctcttcgcc gggctggcgc tgggcctgat
     1381 cggcctggac cagatttcgg cgcaggcccg ctacaccggc ggcatccccg agctgctgga
     1441 cggcatcgag atcgtcctgg ttgcggtggg cctgttcgcg gtggccgagg tactttattt
     1501 cgcgctgtac gagggtcgcg tggtcgagac acgcaaccgg atgagcaggg tgtacatgtc
     1561 ggcgcgcgac tggaagcgct cggtcccggc gtggctgcgc gccacggcta tcggatcgcc
     1621 tttcggctgc atcccggccg gcggcaccga aataccgacc ttcctgagct acgcggtgga
     1681 gaagaagctg gccaagggcg aggacctcaa ggagttcggt accaccggcg ccatcgaggg
     1741 cgtggccggg cctgaagcgg ccaacaatgc gacggtgacc acggcgatga ttccgctgct
     1801 gacattgggc atcccgatct ccagcaccac cgcgatcctg ctcggagctt tccagaacta
     1861 tggcatccag cccgggcccc agctgttcac gagctcggcg gcgctggtct gggcactgat
     1921 agcctccctg tatatcggca acgtcatgct gctggtgctg aacctgccga tggtcggcct
     1981 gtgggtgaag ctgctcaaga ttcccaagcc ccagctctac gcgggcatcc tgatcttcgc
     2041 gaccgtcggc gcctatggaa tgcgccagag cgcgttcgac ctcgtgctgc tgtgggcaat
     2101 cgggatcgtg ggcgtgctga tgcggcggtt cgattttccg acggctccgg tggtcgtggg
     2161 gatgattctc gggccgctgg cggaagccca gttgcgcaac gcagtgtcca ttggcgaagg
     2221 cagcgcgatg gtgttcctgc aacgtccgat gtccgcggtg ctggtggtgc tcatcgtggc
     2281 cgtgctgctg ctgccgcgcc tgtacaagct catgcaacgc cgcaggcccg cgccggccgc
     2341 ctgaacgcta cggcgcggta acatccgcgc atgcaagaaa cggactccct gccgcgcgac
     2401 ggccagagca tcctggaact ggcggcgcgc tcgatgttcc acctgttctc cagcatcagc
     2461 cagggcatgt tcctggtgga ccgcaccggc cgcatcgtct ggctcaatga gggctacaag
     2521 cgcttcctgc cggcgctggg tttttcctcg gtggaccagg tcgtgggcca catggtcgag
     2581 gacgtcgtgc ccaacaccca gatgcgccgc gtactggaga cgggcgagcc gatcctgatc
     2641 gacctgctca ccaaccgcgc gggtaccttc gtggtcagcc gcattccgct gcgtgacgac
     2701 cagagcggcg agctcatcgg cgccatcggc atcgtgctgt tcgaccaccc tgaaaccacg
     2761 ctgcagccgc tgatcagcaa atttgcgcgc ctgcagcgcg acctggacga tgcccggcgt
     2821 gaactggcga gccagcgccg caccaagtac accctggcca gcttcgtggg gaccagcccg
     2881 gcgacagtcg aagtcaagcg gcaggcaaga cgcgcggcgc tctcgtccag cccggtgctg
     2941 ctgctgggcg aaaccggcac cggcaaggaa ctgctggccc atgcgatcca tgccgcatcg
     3001 gcgcgctcgg ccgggccgct ggtcagcatc aacatcgcgg cggtgcccga cacgctgctg
     3061 gaggcggagt ttttcggggt cgcgccgggc gcgtacaccg gcgccgaccg aaagggccgc
     3121 gtcggcaagt tcaagcttgc ggacggcggc acgctgttcc tggacgagat cggcgacatg
     3181 ccgcccagcc tccagcccaa gctgctgcgg gccttgcagg agggcgagat cgagcagctc
     3241 ggctcgaaca aggtcgtttc cttcgacgcg cgtgtgatag ccgccacttc gcgcgacctg
     3301 gcggcactgg tgcgcgaggg aaagttccgc gaggacctgt attaccgctt gaacgtcctt
     3361 ccgatccggg ttcccccgct gcgcgagcgc aggcccgaca ttccggccct ggtcgaagtg
     3421 ctggccgagg acatcgcctt gcgcggcggc tcggcgccgc tcgaactcgg cgccgatgcc
     3481 atcgcgctgc tgtcggcgca ggattggcgc ggcaacatcc gcgagctgcg caacgtgatg
     3541 gagcaggccg cgatgcgcag cgactcgcag cgcatcgaag tccagcacct cgcgcaggtg
     3601 ctgcgcgaat ccggcgtcaa gaacattgct gcgccagccg tcgcatcgga gcccatcccc
     3661 ccgtcctcgc aggtcctgcg gcccctggct gagcaggtcg cggaactgga gcggcgcgcc
     3721 atagacgccg cgttgacagc caccggcggc aacaagctgg cggctgcgaa gctactgggc
     3781 atttcgcgcg ccaagctcta cgagcgcctc gacggcgtga ctgattttca gacagtgtct
     3841 gttaataaga caatcaggat tgcctgattg tcaggcacgc ccgcgtgtgc ggtgaaaaat
     3901 atgttgaaaa atcaacagtg tttccgctgg catggcccgt gcataactgg tccgttcaaa
     3961 actgaagtcc ttcgaaggag acatccatgc aacgtcgcac ctgggtggcg ctcgccgcgc
     4021 tcgcagcttc cgctttcacc ttgcccgtgg ctttcggcca ggccaaggat atcaagatcg
     4081 cgcatatcta cagccgtacc ggcccgctgg aagcgtatgg caagcagacg gccacgggcc
     4141 tgatgatggg gctggactac gccaccaacg gaaccatgag cgtgaacggt cgaaagatca
     4201 ccgtgatcga aaaggacgac cagggcaagc cggacctggg caagtcgctg ctggccaccg
     4261 cgtattcgga cgacaaggtg gatctggcgg tgggaccgac ctcttcgggc gtggcgctgg
     4321 ccatgctgcc tgtcgccgag gaatacaaga agatcctgct ggtcgagccg gccgtcgcgg
     4381 attcgatcac cggcgacaag tggaacaagt acatcttccg cacggggcgc aacagctcgc
     4441 aggacgccat ctccaacgca gtcgcgctgg acaagcccgg cgtgaccatc gccacgctgg
     4501 cgcaggacta cgcgttcggg cgcgacggcg tcaaggcctt caaggagtcg gtcaagaagg
     4561 ccaagttcgt ccacgaggaa tacctgccgg cgaacacgac cgatttcacg gcgggcgcgc
     4621 agcgcctgat cgacaagctc aaggaccagc cgggccgcaa ggtcatctgg gtgatctggg
     4681 cgggcgcggg caaccccttc aagatcgccg acctcgacct gaagcgctac ggcatcgaga
     4741 tcgcaacggg cggcaacatc ctgcccgcca tgggggcgta caagcagttt ccgggcatgg
     4801 aaggggcgac gtactactac ttcgggattc ccaagaaccc cgtcaacgag gccctggtag
     4861 ccagccatta caagcagttc aagagcccgc cggacttctt caccgcgggt ggtttcgcgg
     4921 ccgcgatggc ggtggtgacg gcgctgaaga aaaccaatgg cgacaccggc acggacaaac
     4981 tcatctccgc gatggaaggc atgagcttcg acacgccaaa ggggaagatg accttccgca
     5041 aggaagacca tcaggccatg caaagcatgt accacttcaa gatcaagtcg gaccccgcgt
     5101 tcgcctgggg cgtgcccgag ctggttcgcg agatcaagcc ggaagagatg aaagtcccga
     5161 tccggaataa gcgatagggg cgttggccca accgacattg tcatgccgga ccccggatcg
     5221 agtccggggc aagccctgat ccggcatcca cctggatcgc gggcttgaca aaaaacactt
     5281 gtcatgccgg acctgatccg gcatccatga cttcctggat tgcgggtcaa gcccgcaatg
     5341 acaaaacttt tgtcatgccg gactcgatcc ggcatccatg ggtgcctgat cggcaccccc
     5401 caaggtcttt tcgaaaaaca ccatgagcct gctagccacc aaggacctca caatccgctt
     5461 cggcgggcac gtggcggtca atgccgtgac ctgcgctttc cagcccggca cgctcacggc
     5521 tatcgtcggc cccaacggcg cgggcaagac cacgtatttc aacctgatct ccggccagct
     5581 cagggcgacc gccgggcagg tcacgctcga tggccgcgac ctgtcgggga tgtcggcttc
     5641 ccagcgcacg catgccgggc taggacgcgc gttccagctc accaacctgt ttccccggct
     5701 cacggtgctc gagaacgtgc ggctggccgt gcaggccgcc cgtgcgggca agcaccggcg
     5761 cgggttcaac ctgtggagca tctggagcga ccatcgcgaa ctgaccgcgc gcgcccacga
     5821 gatcctgcgg gccgtggcgc tggcacccaa ggaacacgaa ctggtggcag gcttgccgca
     5881 cggcgaccag cgcaagctgg aggtggcgct gctgatgggc ctggaatcgc gggtgttcat
     5941 gttcgacgag ccgaccgccg gcatgaatgc ggcggaagtc ccggtgatcc tgaacctgat
     6001 ccgcaagctc aaagaggacg cgagcaagac catcctgctg gtcgaacaca agatggacgt
     6061 agtgcgcgaa ctggcggacc gcatcatcgt cctgcacaac ggcgcgctgg tggcggatgg
     6121 tgatccagcc gcggtgatcg cgtcgcccat tgttcaagaa gcttacctgg gggtagcccc
     6181 gacggttcgg gccgggcccg tcgaagcggt cccaggcgcg agcagggaag ccgcatgaac
     6241 gagaacatgc tgtcgctgca aggcgtgcat acgcacatcg gggcctatca catcctgcac
     6301 ggcgtcgacc tgacggttcc ccgcggcaag ctcaccatgc tgctgggccg caacggggcc
     6361 ggcaagacga cgacgctgcg cacgatcatg ggtttgtggc acgcctcgca gggacggatc
     6421 acgttcaacg gccaggacat cacgcagctg gccaccccgc agatcgcctc catgaacgtg
     6481 gcttacgtgc ccgagaacat ggggatcttc tccgatctct ccgtcaagga aaacatgctg
     6541 ctcgccgcgc gttgggcgag gaacgcgggc cagatcgacg ccgagcgcct gaagtggatc
     6601 ttcacgctgt tccccgcggt gcagacgttc tggaaccatc cggcgggcaa gctctccggc
     6661 ggacagaaac agatgctcgc cgtcgcgcgc gccatcgtgg agccacgcga actgctgatc
     6721 atcgacgagc cgagcaaggg cctggcgccc gcgatcatca acaacatgat cgacgccttc
     6781 ggccagctca aggccagtgg cgtgacgatc ctgctggtgg agcagaactt caacttcgcc
     6841 aagcgcctgg gcgatacggt ggccgtgatg gacaacggcg tgatcgtgca tgccggttcc
     6901 atgaaggaac tggccgagga cgaggccttg caacactccc tgctggggct ggcaatatga
     6961 acaaaatcga cttcgactgg aagccgctcg cgctggtgcc ggtgctggcg ctgctggtgc
     7021 tgcccttcat cggttcgggc tccacctggg tcacgctgac cgtggcgggc ctggccatgg
     7081 gcatgatcat cttcatcatc gcttcgggcc tcacgctggt gttcggcctg atggacgtgc
     7141 tgaacttcgg ccacggcgtg ttcatcgcgc tgggtgcctt tgtcgccacc agcgtgctcg
     7201 gcgccatggg cgactacacc gcgtccgcca gcctgtggcg caacatgatg gcggtgttgc
     7261 cggccatggt ggtcgcgatg ctggtggccg gggctgtggg tgtggcgttc gagcgcttca
     7321 tcgtgcgccc ggtatacggc aatcacctca agcagatcct gatcaccatg ggcggcatga
     7381 tcatcggcga ggagctgatc aaggtgatct ggggaccgct gcagatcccg ctgccgctgc
     7441 ccgagggcat gcgtggttcc atcttcctgg gcgacgccgg cgtggagaag taccgcgtca
     7501 tcgccgtggt ggtggggctg ctcgtcttcg gcatcctgct gtgggtgctg acgcgcacca
     7561 agatcggcct gctgatccgc gccggagtgc aggaccgcga gatggtggaa tcgctcggtt
     7621 accggatcaa ggtgctgttc gtcggcgtgt tcgtggtcgg cagcgcgctg gccggcctgg
     7681 gcggcgtcat gtggggcctg taccagcaga gcgtgatccc gcagatgggg gcgcaggtca
     7741 acatcctgat tttcatcgtg ctgatcatcg gcggcctggg ttccacgacc ggcgcgctga
     7801 ttggcgcgtt gctggtcggc ctgatggcca actacaccgg attcctggtg ccgaaggcgg
     7861 cgctgttctc gaacatcgca ctgatgatgg ccatcctgct gtggcgtccg cagggtgtct
     7921 acgcgctcag ccgttgatcg tttccaccat gctgaaccgc ctgatttcca acgactaccc
     7981 gcgcagccgg gtgctcgccg tgctgctggt cgcaatcctg ctggcactgg cattcacgcc
     8041 gttcatcgtt cccggcgtga aggcgctgaa tgtggcggcc aagatcctcg tgttcatcgt
     8101 gctggtcgcc agcttcgacc tgctgctggg ttacaccggc atcgtgagtt tcgcccacac
     8161 catgttcttc ggcatcggcg cctacggcat tgcgatcgcc tccacccgca tgggaccggg
     8221 ctggtccgcc atcctcgccg ggctggcggt ggccctggcg ctgtccttcg tgctggcgct
     8281 ggccgtgggc ctgttctcgc tgcgggtgcg cgccattttc ttctccatga tcacgctggc
     8341 ggtggcggcg gcgttccaga cgttggcgtc gcaactgtcc gagatcaccg gcggcgagga
     8401 cggcctcact ttcaagatgc ccgaactgct gtcgcccggt ttccagctct cggagtcgcc
     8461 cttcctgggc gtctcgctgg acggccggct gatcagctac tacctgctgt tcttcaccac
     8521 gctggtgctg ttgctggcca tgctgcgcat cgtcaattcg ccgttcggcc gcgtgctgca
     8581 ggcgatccgc gagaacgagt tccgcgccga ggcgatcggc taccgggtgg tgctctatcg
     8641 cacgacttcc agcatcctgt cggcgctgtt cgcgaccgtg gccggcgcga tgctcgccat
     8701 ctggctgcgc tacaacggcc cggacacgtc gctgtctttc gaaatcatgc tggacgtgct
     8761 gctgatcgtg gtgatcggcg gcatgggaac gatctacggc gccgccatcg ggtcgggcgt
     8821 gttcgtggtg gcgcaaagct acctgcagga cctgcttggt ctcggcagcg atgccacctc
     8881 cggactgccc tggttatcgg cactgctctc gccggaccgc tggctgctct ggctgggcct
     8941 gcttttcgtg ctctcggtct attactttcc gaccggggtg gtggggcggc tgcgagcgat
     9001 gggagcgcgc ggctgatgcg aacgcgcccc tgaccgcagc aataaattgt tcattgagag
     9061 gtgcatgcca tgcagtccag ctcctgttat gcgacctgcg cgggccgcga gatccattac
     9121 accgagtggg gggcgcagca ccgccgcacc gtcatcgcct ggcatggcct ggcgcgcacc
     9181 ggccgggaca tggacgaact cgcgcagcat ctttccagcc gctttcgcgt gatctgcccc
     9241 gacacgctcg gccggggcct gagccaatgg agccccgacc cgggaaacga atacacgctg
     9301 gcgttctacg ccaggctggc ggcggacctg ctcgaacagc tcgatatcgg cgaggcgcac
     9361 tgggtcggca cgtcgatggg gggctcgatc gggaccgtgt gtgccgccgg cctgctgcag
     9421 ccgcaactca agggccgtat caggagcctt gtcctcaacg ataacgcgcc gcacctcgcc
     9481 gagccggcgg tggcgcgcat ccgcgcatat gccggcaacc cgccggcatt cgagaccatc
     9541 gccgaactgg aggccttctt ccgccaggtc tacaagccgt tcggctggct cagcgacgcg
     9601 cagtggcggc ggctgaccga aacctccacc cggcggctgc ccgatggacg ggtcacgccg
     9661 cactacgacc cggcgatggt acggcagttc atcgaccacc ccagggatta cgagctgtgg
     9721 gaccactatg acgccatcga catccccgtg ctgtgcctgc gcggagccga atcggatctg
     9781 gtgctgcgcg aaacgaccga tgagatgttg cgcaggggcc ccggatcgcg cggcctgaca
     9841 agggttgtgg aggtcgcggg ctgcggccac gcaccggcgc tcaacgtgcc cgaacagctc
     9901 gagctggttg cctcgttcat cgagcagcgc gacgacgctg tcgcgccggc ggcgccggga
     9961 actaccgggc cgctccgctg gccatagcgg cttcgcgcga ctcgcgcagg acgtcggcgt
    10021 ccggcgtctc gcgcgtgggc cagccggcga ggtgccgttc ggccacgcag cccagtgccg
    10081 ctatccagcc ccggtcgtcg ttcaggcagg caatgtagtg gaactccttg ccgcccgagg
    10141 tgaggtacgc ctggcggcct tcgatggcga tctcttccag cgtttccagg cagtcgccgg
    10201 tgaagccggg gcagaccacg tcgacacgcc cgatgccgcg ccgcgccagc gcgcgaagcg
    10261 tcggctcggt gtagggctcc agccatttgg ccttgccgaa ccgggactgg aaagtcaacg
    10321 tgtaccgctc tttctcgagc ccgagatgcg aggccagcag gcgcgccgtt ttctggcatt
    10381 cgcagtgata agggtcgccc aggcgcagcg tgcgttccgg caccccgtga aagctcatga
    10441 ccaggtgttc gccgcggccg tgttcgcgcc aataaccctc gatgcgccgg gccagcgcct
    10501 ggatgtagcc gcggtcgtcg tggtagcgat tgacgaaacg cagctcgggc acgctccgga
    10561 tgcccgtcga ccacgctgcc acggcgtcca cgaggctcgc ggtgctggtg cccgagtact
    10621 gggggtaggc gggcaggacg aggatgcgtg tcgcgccacc ggctttcagc gcgtcgagct
    10681 gggaggcgat cgaagggttg ccgtagcgca tcgcgaaacg aacttttacg tgatggccgt
    10741 gttcgcccag ccagccctgc agcagcttgg cctgcttgtc ggtccacaac aacaggggcg
    10801 agccgtcggc ggtccagatc ttcgcgtact tggctgccga cttgccggag cgaaacggca
    10861 ggatcagtcc gtgcaacaaa ggccaccaca gcagcctcgg gatctccacc acgcgcgggt
    10921 cggcgaggaa ttccaacagg tagcgccgca cggcccccgg cgtcggctcg tcgggcgttc
    10981 ccagattgca atacagcacc gcgatgcccg gcgctctgcc gtggctgaat tcgggttcct
    11041 gctgaaagga gggggaagcg gccatgcggt attctcactg aatgagcccc cacgttcact
    11101 atcgttctct gccccccgag gggccgcatg cctgcctaga ggcggctcgc cggaaggcat
    11161 gatgctggat gtctcgaaaa tcaaggcgat ctcgatcgac cttgatgaca ccctttggcc
    11221 gatctggccg accatcgaaa gggccgagaa ggtgctgcac gactggatgg tcgagtacgc
    11281 accgatggcc gccgcgctgt tctccagccc gtcggcactg cgcgacatcc gtaaccacat
    11341 ggccgcgagc cggccggacc tgaagaacga cctgagcgcg gtacgccgcg agtcgatccg
    11401 gctggcgctg taccgtgccg gcgaaaaccc gctgctggcg gaccaggcct tcgaggtgtt
    11461 cttcgccgaa cgccagcgcg tcacgctgtt cgacgacgtg cagccgtcgt tgcagttcct
    11521 gtcggcgcgg ttcccgctgg tgagcgtgtc caacggcaac gccgacctgg agcgtgtcgg
    11581 cctgggctcg tatttccgcg ccgccgtttc ggcccgcgag ttcggcgtcg gcaagcccga
    11641 tccgcgcatc ttccacgcgg ccgccggcgc ggtggacctc acgaccgagc atgtgctgca
    11701 cgtgggggac gatgcgacgc tggatgcgct cggcgcgctc aacgccggca tgcaggcggc
    11761 ttgggtcaat cgcttggacc acctgtggcc ccacgagctg gagccgcacg tgaagttgac
    11821 caacctgacg gagctctgcg ccctgttcaa gtgaccgctg cggcgggcgc agcccgccat
    11881 aagcggtcat cccggcgcag gtgacgccgc gaccgcggcg tcatcccggc gaaagccggg
    11941 acccagtatt cctcacagcc ttcccagtgc ctgcaccttc caggccagcc agaatttgcg
    12001 cagcggtgtc aggctcacgc gctggtgcag cacct
//
LOCUS       DASWIY010000214         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_502359, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000214 DASWIY010000000
VERSION     DASWIY010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502359"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VGA59_07840"
     CDS             <1..224
                     /locus_tag="VGA59_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719630.1"
                     /translation="FINSKVEELSGGKPFRHIDDLISQEQLRRISEDYKRIAGFSLDE
                     PDENTVDAGSAGHDTPLSTSVIAGSTRNP"
     gene            complement(308..886)
                     /locus_tag="VGA59_07845"
     CDS             complement(308..886)
                     /locus_tag="VGA59_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719631.1"
                     /translation="MKIHQLSVTYIAEQDRILMRVNTATAEEMRLWLTRRLMLGLWPL
                     LSKLLTKHLLKLEAAGTSLETADEDLKKMLAEFRKEEFLKQADFETPYQENQPQLPLG
                     EEPLLVTDVDASPLPNGQLRLSFNERPAGEGKPRSFRMEMEPKLMQGLMHLLEQALAR
                     SQWREAFGTPTAVEDGISADEDRASRKPRYLN"
     gene            1089..>2663
                     /locus_tag="VGA59_07850"
     CDS             1089..>2663
                     /locus_tag="VGA59_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX9719632.1"
                     /translation="MRLDKLTTKFQEALGEAQTLALGNDHAYIEPEHLLVAMLRQQDG
                     PRALLQRAGVNVPGLLAAAESAMHRRPKVEGQEQVQVGRDLVSLLQAAEKEAIKRGDQ
                     FIAGELFMLAVADTKQDIGRIARENGLSRKSLESAVDAVRGGQNVDSPEAEGQREALK
                     KYCIDLTERARMGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIIAGEVPETLKNKRVLSLDMAALLAGAKFRGEFEERLKNVLNELAKDEGQTIVFID
                     ELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLGEYRKYVEKDAALERRFQKI
                     LVDEPSVEATIAILRGLQEKYEVHHGVEITDPAIVAAAELSHRYITDRFLPDKAIDLI
                     DEAASKIKIELDSKPEVMDKLDRRLIQLQIEREAIKREKDEASRKRLALIEEEIGRLQ
                     KEIADLEEIWKAEKAQAQGSAQVKEEIEKLRGQIEEFTRKGDFNKVAELQYGRLPELE
                     KRLKEAQATETSKAKNA"
BASE COUNT          560 a          823 c          868 g          412 t
ORIGIN      
        1 atttcatcaa cagcaaggtc gaggaactca gcggtggcaa gccgttcagg cacatcgacg
       61 acctgatcag tcaggaacag ctgcgccgca tttccgagga ctacaagcgc atagccggct
      121 tttcgctgga cgagcctgat gaaaataccg tggatgccgg atcagccggg catgacacac
      181 ctctgtcgac ctctgtaatt gcgggctcga cccgcaatcc atgactccgg aaatctgaat
      241 cccggatcag gtccggatca ggtccgggat gacgctttgt cttgcgggct tcgcccgcca
      301 tcaagtgtca gttcaggtag cgcggcttgc gcgacgcgcg gtcctcatcg gcggaaatgc
      361 catcctcgac cgccgtgggc gtgccaaaag cctcgcgcca ctgcgagcgc gccagtgcct
      421 gctccagcaa gtgcatcagg ccctgcatca gcttgggctc catttccatg cggaagctgc
      481 ggggcttgcc ctcgccggcc ggtcgctcgt tgaaactcag gcgcagctgc ccgttgggca
      541 ggggcgacgc gtccacatcc gtgaccagca gcggctcttc cccgagcggc aactgcggct
      601 ggttctcctg gtacggtgtt tcgaagtccg cctgtttcag gaattcctcc ttgcggaact
      661 cggccagcat tttcttcagg tcctcgtcgg ccgtctccag ggaggtgccg gccgcttcca
      721 gcttgagcag atgcttggtc agcagcttgg acagcagcgg ccaaagcccc agcatcaggc
      781 gccgggtcag ccacagccgc atttcctcgg cggtggcggt attcacccgc atcaggatgc
      841 gatcctgctc ggcgatgtag gtcactgaca gctggtggat cttcatgcct cgattctagg
      901 gcctgctcac actgttttta ccagtgcgag tggggtgaga accgcgccaa tcaaggcgcg
      961 ggacaacggc aaggctggcc gccttgccta ggagacccta gtcatccgtg gggcgcgata
     1021 gaagcgcgca ttgacccttg aaaagctggg gacaagccta agctgggaag gtgaacggag
     1081 agatacacat gcgactagac aagctcacca caaaattcca ggaagccctg ggcgaggcgc
     1141 agaccctggc cctgggcaat gaccacgcct acatcgagcc cgagcacttg ctggtggcca
     1201 tgctgcgtca gcaagacggg ccgcgggccc tgctgcaacg cgccggcgtc aacgtgcccg
     1261 ggttgctggc ggcggccgag tcggccatgc acaggaggcc caaggtcgag ggccaggaac
     1321 aggtccaggt cggccgggat ctggtgtcgc tgctgcaggc cgccgagaag gaggccatca
     1381 agcggggcga ccagttcatc gccggcgaac ttttcatgct ggctgtcgcc gacaccaagc
     1441 aggacatcgg ccgcattgcc cgtgaaaacg gcctttcgcg caaatcgctg gaatcggccg
     1501 tggacgccgt gcgtggcggc cagaacgtgg atagcccgga agcggaaggc cagcgcgaag
     1561 cgctcaagaa gtactgcatc gacctgaccg agcgcgcccg catgggcaag ctcgaccccg
     1621 tgatcggccg cgacgacgag atccgccgcg cgatccaggt gctgcaacgc cgcacaaaga
     1681 acaatcccgt gctgatcggc gagcccggcg tgggcaagac cgcgattgtc gaaggcctgg
     1741 cgcagcgcat cattgccggc gaagtgcccg aaacgctgaa gaacaagcgc gtgctgtcgc
     1801 ttgacatggc ggcgctgctt gcgggcgcca agttccgcgg cgaattcgag gaacgcctga
     1861 agaacgtgct caacgagttg gccaaggacg aaggccagac catcgttttc atcgacgagc
     1921 tgcacaccat ggtgggcgcc ggcaaggccg aaggcgcgat ggacgcgggc aacatgctca
     1981 agccggcgct ggcacgcggt gaactgcact gcgtgggcgc gaccacgctg ggcgaatacc
     2041 gcaagtacgt cgagaaagac gcggcgctgg agcggcgctt ccagaagatc ctcgtcgatg
     2101 agccgagcgt ggaggcgacc atcgccatcc tgcgcggcct gcaggagaag tacgaggtgc
     2161 accacggcgt ggagatcacc gacccggcca tcgtcgccgc ggccgagctg tcgcaccgct
     2221 acatcaccga ccgcttcttg cccgacaagg cgatcgacct gatcgacgaa gcggcatcca
     2281 agatcaagat cgaactcgat tccaagcccg aggtgatgga caagctggac cggcgcctca
     2341 tccagttgca gatcgagcgt gaagccatca agcgcgaaaa agacgaggcc tccaggaagc
     2401 gtctggcgct gatcgaggaa gagatcggcc ggctgcaaaa ggaaatcgcc gacctggaag
     2461 aaatctggaa ggccgagaag gcgcaagccc agggctccgc ccaggtgaag gaagagatcg
     2521 agaagctgcg cggccagatc gaggagttca cccgcaaggg cgacttcaac aaggtcgcgg
     2581 agctgcaata cggccgcctg ccggagctgg agaagcggct caaggaagcg caggccacgg
     2641 aaacgagcaa ggcgaagaac gcc
//
LOCUS       DASWIY010000215         9858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_120847, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000215 DASWIY010000000
VERSION     DASWIY010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120847"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VGA59_07855"
     CDS             <1..715
                     /locus_tag="VGA59_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX9719633.1"
                     /translation="GKSTSLAAMVDWRNENSQGHIITIEDPVEFVHPHKNCVVTQREV
                     GLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFAETGHLCLATLHANSANQA
                     LDRVINFFPEERRAQLLMDLSLNLRAFVSQRLVPKQDSKGRSAAVEIMLNTPLVSDLI
                     FKGEVSEIKEIMKKSRNLGMQTFDQALYDMYENNIITYEDALRNADSLNDLRLQIKLN
                     SQRAKSQDLSAGTEHLAIV"
     gene            891..1793
                     /locus_tag="VGA59_07860"
     CDS             891..1793
                     /locus_tag="VGA59_07860"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9719634.1"
                     /translation="MASTNPKTYESTPSRKVAFLGLGVMGFPMAGHLALAGHDVTVYN
                     RSPAKAQAWTGEFKGGAAPTPRAAAAGRDLVFCCVGNDDDLRSVVLGDDGALAGMKPG
                     AVLIDHTTASANVARELYAQAGKRGLQFVDAPVSGGQAGAQNGLLTVMCGGDAAAFEA
                     AKPVAMAFSRAFTLLGEAGSGQLAKMVNQIAIAGLVQGLSEAITFGQQAGLDMAQVLE
                     VIGKGAAQSWQMDNRGKTMIAGKFDFGFAVDWMRKDLGLCLDEAKRNGARLPVTALVD
                     QFYADVQAMGGRRWDTSSLITRLK"
     gene            complement(2204..2800)
                     /locus_tag="VGA59_07865"
                     /pseudo
     CDS             complement(2204..2800)
                     /locus_tag="VGA59_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
     gene            complement(2797..3396)
                     /locus_tag="VGA59_07870"
     CDS             complement(2797..3396)
                     /locus_tag="VGA59_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEX9719635.1"
                     /translation="MLELRIQQHFIDSADLKYQSAQSLSKPIAAAVHAILACVTSGGK
                     VLACGNGGSAADAQHFSAEFVGRFERERPELGAIALTTDSSILSAIANDYDFERVFSK
                     QVRALGQAGDVLLALSTSGNSANVLAAIEAAHEREMTVVALTGRGGGKITNALRETDV
                     HICVPHDRTARIQEVHILALHCICDGVDAQLLGEQEPPT"
     gene            3511..3663
                     /locus_tag="VGA59_07875"
     CDS             3511..3663
                     /locus_tag="VGA59_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9719636.1"
                     /translation="MSEYRIRRLPIVNRDKRLVGILALGDLAVKEPDKQPAAEALCEI
                     SEHAHA"
     gene            4107..4478
                     /locus_tag="VGA59_07880"
                     /pseudo
     CDS             4107..4478
                     /locus_tag="VGA59_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451141.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
     gene            complement(4510..4902)
                     /locus_tag="VGA59_07885"
     CDS             complement(4510..4902)
                     /locus_tag="VGA59_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX9719637.1"
                     /translation="MEFPRKKDTAAAATTKQAGDAAEDRALAHLLDHGLKLLARNYRT
                     PGRGGGEIDLVMREPGGTLVFVEVRQRAGRSFGGAAASVSHAKRQRIIFAARHYLMRL
                     AQPPPCRFDVVSVEAGAVEWLRGAFDAS"
     gene            4902..5819
                     /gene="rsmI"
                     /locus_tag="VGA59_07890"
     CDS             4902..5819
                     /gene="rsmI"
                     /locus_tag="VGA59_07890"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX9719638.1"
                     /translation="MSASFASALNAARDAAASQHYPQGALYVVATPIGNLADISLRAL
                     HALQLADAIACEDTRHTQALLRAYGIDKSAAQWLAVHQHNEAQGAQGVIERLRQGQRV
                     AYASDAGTPAVSDPGARLAAAVRAAGLRIVPLPGASSVTAVLSVAGAVADDGAFLFAG
                     FLPAKAGERQAAVARLAQESRPVVLLEAPHRIEASARSLERLGPRPVTVGRELTKQFE
                     EIATLACAELPGWLAADPRRSRGEFALVLHAAPQARQGDDDLRVLTLLLGELPVKIAV
                     KLAAEITGGSRNALYQAALKLKSDGDAPA"
     gene            complement(6035..7930)
                     /locus_tag="VGA59_07895"
     CDS             complement(6035..7930)
                     /locus_tag="VGA59_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9719639.1"
                     /translation="MTPSWPTSRPGRWARAVLVAVGLACTAATMMAAVLEPSHGSMLT
                     APDAAPPARDRAWQTVPLPDIWRRSRPGVPPADTWYRMDFDFSEGPQPAEPWALYLPY
                     LYDGGEVWLNGALAGRVTQKSRLVHIRWARPHLVTLPSSLLRAGRNEVAVRTALPRGG
                     AAVGFPRPGIGPQAELSPLYERRFFWVNTAPLITASLCLLVSGFVLFIWWRRRNEVLY
                     GLFGLAAGLWGVRTLTFVIEALPVERWLLWRLTYLGATGGFIVAMAVLALRFAGIRRP
                     WAERGLLLYWLVGPLWVLAGGAAAEPLVNRYWIAGFLPIGIAIVAISLWTVWRQRTLM
                     AAVMPVAMVLAVLAGLHDYLVNWNVGGAVPLLPTGWAGHRIFLLHHGANLVLVAMGGL
                     LTARFIQALTGLEGLNQTLESRVADRERELSANYAQMAELQRQHAAAQERQLIMREIH
                     DGLGSQLFVSLSRVERGDMQGAQIADALRGCIADMRLALDTLTPDESDFRSTLGDFLF
                     RWQGQLLAAGIHPAWTIDVPDGALNLTPHAALQLLRIAQEALTNVLKHAAATRVQVRL
                     RQAGDLLELEVEDDGRGASAQPGASGRGIGNMRARAQQLGGQLELRNFDKGTCVALRV
                     PMKAVLA"
     gene            complement(7927..8586)
                     /locus_tag="VGA59_07900"
     CDS             complement(7927..8586)
                     /locus_tag="VGA59_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9719640.1"
                     /translation="MRTSVLIVEDEPEFMRRFSNAVLADPGLNLVGAVSTGRAAQAML
                     DAERPDVMLADLGLPDMNGVEVIRYAAAHHPQCDVLVVTMFGDDQHVIGSIEAGATGY
                     LLKDAVAERIAASIHELRAGGSPISPGIARRVLARFRVATPPATGADPGPAATPSPLT
                     ERETELLRLTAKGLSFDTIGQLMGISPHTVVAHVKKIYRKLAVHSRGEAVYEAGQLGL
                     L"
     gene            complement(8702..>9858)
                     /gene="priA"
                     /locus_tag="VGA59_07905"
     CDS             complement(8702..>9858)
                     /gene="priA"
                     /locus_tag="VGA59_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX9719641.1"
                     /translation="RRLVMASRVGQDGGSGAPDANAGASGAFPCVRLVDMSLQPKHTV
                     FSPQLLEAIQQRIARGEQSLVFLNRRGYAPVLACTACDWKSECPHCSAYRVFHKIDRT
                     LRCHHCGYTERVPRACPSCGNVDIAPLGRGTERLEEQLADLLAEVKQSNDEAVRIARM
                     DADSTRLKGALEQQLARMHAGDVDVLVGTQMVTKGHDFRRITLVAALNADSALFSSDF
                     RAPERLFSLLMQAAGRAGRDAAHAQASEMWVQTWHPRHPLFKALRSHDYPAFAAQQLK
                     EREQAGMPPFGFQALLRAEARSQEAAQAFLRAASAAAAGLDGAQRVTLYSAVPMSIQR
                     VANVERAQMLVESASRSALQRFLAAWQPMLQATREKGLIRWAVDVDPLAI"
BASE COUNT         1596 a         3351 c         3448 g         1463 t
ORIGIN      
        1 gggcaaatcg accagcctcg ccgccatggt cgactggcgc aacgagaatt cgcagggcca
       61 catcatcacc atcgaagacc cggtggagtt cgtgcatccg cacaagaact gcgtggtcac
      121 ccagcgcgag gtggggctgg acaccgacag ctgggaggcc gcgctgaaga acacgctgcg
      181 ccaggcgccc gacgtcatcc tgatgggcga aatccgcgat cgcgagacca tggagcacgc
      241 catcgccttc gccgaaaccg gtcacctgtg cctggccacg ctgcacgcca acagcgccaa
      301 ccaggcgctc gaccgggtga tcaacttctt ccccgaggag cggcgggcgc agctgctgat
      361 ggacttgtcg ctcaacttga gggccttcgt ctcccagcgc ctggtgccca agcaggacag
      421 caagggccgg tcggcggccg tggagatcat gctgaacacg ccgctggtgt cggacctgat
      481 cttcaagggc gaggtgtcgg agatcaagga aatcatgaag aagagccgca acctgggcat
      541 gcagaccttc gaccaggccc tgtacgacat gtacgagaac aacatcatca cgtatgagga
      601 cgcgctgaga aacgccgact cgctcaacga cctgcggctg cagatcaagc tcaactcgca
      661 gcgtgccaag agccaggacc tttcagcggg caccgagcat ctggccatcg tttgactcga
      721 aaaacgctgc gcgtttttcg agttgcctga tcgcatgaga aaggccgggg aaaccccggc
      781 ctttctcatg gtgcggctgc gctgcgcgtt gcttcgcgca gccggggccc ctgcggggag
      841 ccagacccgc tccgtcctag aattgcgatt caacacgacc ggagaagtac atggcttcga
      901 ccaatcccaa gacctacgaa tccacgccgt cccgcaaggt cgccttcctc ggcctgggcg
      961 tgatggggtt ccccatggcg gggcacctcg cgctcgccgg ccacgacgtg acggtctaca
     1021 accgcagccc ggccaaggcg caggcctgga ccggcgagtt caagggcggc gcggcaccga
     1081 cgccgcgcgc ggcggctgcc ggccgagacc tcgtgttctg ctgcgtgggc aacgacgacg
     1141 acctgcgttc ggtggtgctc ggcgacgacg gcgccctggc gggcatgaag cccggcgccg
     1201 tcctcatcga ccacaccacg gcgtcggcca acgtggcgcg cgagctttac gcgcaggccg
     1261 gcaagcgcgg gctgcagttc gtcgacgccc cggtgtccgg cggccaggcg ggcgcgcaaa
     1321 acggcctgct taccgtgatg tgcggcggcg atgcggcggc attcgaggcg gccaagccgg
     1381 tggcaatggc tttttcgcgc gcgttcacgc tgctcggcga agccggctcg ggccaactcg
     1441 ccaagatggt caaccagatc gccatcgccg gcctggtgca gggcctgtcg gaggcgatca
     1501 ccttcggcca gcaagcgggg ctggacatgg cgcaggtgct ggaggtcatc ggcaagggcg
     1561 cggcccagag ctggcagatg gacaaccgcg gcaagaccat gatcgcaggc aagttcgact
     1621 tcggcttcgc cgtcgactgg atgcgcaagg acctcggcct gtgcctggat gaggccaagc
     1681 gcaacggcgc ccggctgccg gtcacggcgc tggtggacca gttctatgcc gacgtgcagg
     1741 ccatgggcgg caggcgctgg gacacgtcca gcttgatcac gagactgaaa tgatcgacat
     1801 gcgatgagtt gtcatgccgg accccggatc aagcctgccc cggacttgat ccggggtacg
     1861 gggcaggccc gatccgaata cgagaggact tcctggatcc cggggccggg ccgcagcgtg
     1921 atgcctcggc acgtgcatag cgtaggcccg gcattgggat cgctccactg tacagctgtc
     1981 gcgcacggac gttacgcgag cccagcccgg gatgacatgt caaccctcac cgcgcagggg
     2041 tcgccggcgg cagcggcgtg ggcactgcgg aacgtggcgc ggccggctgc gtgacggcgg
     2101 gcgccgtggt atccggggcc ggggccgggg ccgcggtggc cggcgccacc ggagcggcgg
     2161 ccggcgcggg agccgcggcg ggccggccca gctggggcac cagccgcctg agctcctcct
     2221 cggtcacgat ctcgaaaatg cgcacgactc gccgcacgcc gtcgatgttc cgggcgatct
     2281 gcgtggcccg atcggcctcg cgctgtgtga cccggcccat cagatagacc acgccgcgtt
     2341 ccgtgacgac cttgaaggcg ccgacataga gattccggtc gtcgaccagc gaagccttca
     2401 cctttccggt gatcagcgtg tcggacgtgc gctgggccag cgtggttgcg gccatcacgg
     2461 ccagctcgtt gacgacgccc ttcacgttgt cgacccggga caccacctgc tcggcggtct
     2521 gcctggcctg ctcgttcggg acctcgccgg tcagcaggac ctggcggttg tagctgttga
     2581 tgttgacgtg ggccccgccc atggattcgc gcaggcggct ggccccgcgc agttcgatgc
     2641 cttcgtcttc cagctgggcg ccggaggtgc ggcgatcgaa ggccatgatg gcgccgacgg
     2701 ccgccccgcc gatgatcagc ggggcgcagg cggacagcgt gccggccagg acggcaatgg
     2761 ccagcgcgag gaccaggggt ttcgggttgt ttttcttcat gtcgggggtt cctgttctcc
     2821 gaggagttgc gcatccacgc cgtcgcagat gcagtgcagc gccaggatat ggacttcctg
     2881 gatgcgcgcc gtgcggtcgt gcggcacgca gatgtgcacg tcggtctcgc gcagggcgtt
     2941 ggtgattttt ccgccgccgc ggccggtgag cgccaccacg gtcatctcgc gttcgtgcgc
     3001 ggcctcgatc gccgccagga cattcgccga attgccgctg gtggacagcg ccagcagcac
     3061 gtcgccggcc tgccccagcg cgcgcacctg cttggagaac acgcgctcga aatcgtagtc
     3121 gttggcgatg gcgctgagga tcgagctgtc ggtggtcagg gcgatggcgc ccagctcggg
     3181 ccgctcgcgc tcgaaccgcc cgacgaattc ggccgaaaaa tgctgggcgt cggcggccga
     3241 tccgccgttg ccgcaggcca gcaccttgcc gccgctggtg acgcatgcaa ggatcgcatg
     3301 caccgccgcc gcgatcggct tgctcaagga ttgggccgac tggtacttga ggtcggcgct
     3361 gtcgatgaaa tgctgctgga tgcgtagttc gagcatggtt ggggcacgat gataaccggc
     3421 gtgccgccct gggcgcccgc gctcgcccga cggcgatcac atggcctggg gacggcgtcc
     3481 tacagaccgg acccagccgc tgttcccatc atgagcgaat accggatcag gcgcttgccc
     3541 atcgtcaacc gcgacaagcg gctggtcggc atcctggcgc tgggagacct cgccgtcaag
     3601 gagccggaca agcaacccgc ggccgaggcg ctgtgcgaaa tctccgagca cgctcacgcc
     3661 tgacgttgtc ggacggggtc ctacagtcct ttggcacccg gccgacagcc aggacctgga
     3721 tcgaaggcta ggctgatatc agatgtggtg cgttcctctg tttccggaaa agcgaagccg
     3781 cgcgaagagc gggcacgaac tgaagggaac ggacatccgc gcataccggc cgagtgaggc
     3841 caactcctcg gtaaattgcc ggcgatgaaa agcgcgccac atcccccgcg cagtacctga
     3901 atccattcgc atccctcccg cgtattgaag gggcgtttca acgcaccctc aatactcaac
     3961 aggacagcga catgcgaacg aagaaactga aaacattcca gctgagcctg ctcgcggcgg
     4021 ccgcctgcga tccggcgcgt actggacagc atagagcctt ctttcgaacg ccatggatcc
     4081 gaacccgctc gaccggcgcc tgatcgcgct catcgaacgc atcgcggccg gcgcaacccg
     4141 caatccagta cgtggcggtg ctggaggacg agcggtcggt cgcgtccatg gtggtgacct
     4201 tcgatcccag gaacagcacg ttgacgctca agcgtttgag cacgttccag gaagggccgg
     4261 aaaagtcgct gcaactgtgg gcgctggcgc catcgggcgg tccccgatca ctcggcgtgc
     4321 tgggcgcaga cgccgtcgtc aagctcacgg cggccgagag ccaggtcagg gccgttccgg
     4381 ccctggcggt cagcctcgag cctaagggag gcgtgccggg cgagcgcggg ccgaccgggc
     4441 cggtgctgtt caagggcgcg ctggtgcaga cgccctgagc gttcgcaccc taaggttcca
     4501 tggcggccgt cacgaagcgt cgaacgcgcc ccgcagccat tcgacggcgc cggcttccac
     4561 actgaccaca tcgaagcggc agggcggcgg ttgggcgagg cgcatcaggt aatggcgggc
     4621 cgcgaagatg atgcgctgtc gcttggcgtg gctgacgctg gcagcggcgc cgccgaacga
     4681 gcggccggcg cgctgtcgca cctcgacgaa gaccagcgtg ccgccgggct cgcgcatcac
     4741 caggtcgatc tcgccgccgc cgcgcccggg cgtccgataa ttgcgcgcca gcagcttcag
     4801 gccgtggtcc agcaagtgcg cgagcgcccg gtcctcggcg gcgtcgccgg cctgcttggt
     4861 ggtggctgcg gccgcggtgt ctttcttgcg agggaattcc attgagcgct tcttttgctt
     4921 cagccctgaa cgccgcgcgc gacgcggcgg cctcgcagca ttatccgcaa ggggcgctct
     4981 acgtcgtggc cacgccgatc gggaacctcg ccgatatcag cttgcgcgcg ctgcacgcgt
     5041 tgcagctggc cgatgcgatc gcgtgcgagg acacgcggca cacgcaggcc ctgctgcgag
     5101 cctatggcat cgacaagtcg gcggcgcagt ggctggccgt gcaccagcac aacgaggcgc
     5161 agggcgcaca gggcgtgatc gagcggctgc ggcagggcca gcgtgtggcc tacgccagcg
     5221 atgccggcac gccggcggtg agcgatcccg gcgcacggct ggccgcggca gtgcgtgcgg
     5281 ccggtctgcg tatcgtgcct ctgcccggcg ccagcagcgt caccgccgtc ttgagcgtcg
     5341 ccggcgccgt cgccgacgat ggcgcgttcc tgttcgccgg cttcctgccg gccaaagcgg
     5401 gcgagcggca ggccgcggtg gcgcggctgg cgcaggaaag ccgaccggtg gtgctgctcg
     5461 aggcgccgca ccgcatcgag gcatcggcgc gatcgctcga acgactgggg ccgcggcctg
     5521 tcacggtcgg ccgtgagctg accaagcagt tcgaggagat cgccaccctg gcctgcgccg
     5581 aattgcccgg ctggctggcc gccgaccccc ggcgcagccg gggcgaattc gcgctggtgc
     5641 tgcacgccgc gccgcaggcc cgccaaggcg acgacgacct gcgcgtgctg acgctgctgc
     5701 tgggtgaatt gccggtcaag atcgcggtga agctggccgc cgaaatcacg ggcggctcgc
     5761 gcaacgcgct gtaccaggcg gccctgaaac tcaagtccga cggcgacgct ccggcgtagc
     5821 atgccggccg ccgaccctca gaacggaacc tttcagggga tggcgcggcg cgacgcaggc
     5881 gtgatctcag tttcccatga atgagaacta cacccacgac ggatgaaagt ggatgctgca
     5941 ggcgcgggcc accgcggaac cggctttgcc gggccgctgg tggcgccccc tcgaggggga
     6001 ggcgccgaag gcgcttcggg ggtgggcttt cacttcaggc gagtaccgcc ttcatgggaa
     6061 cccgcaacgc gacgcatgtc cccttatcga agttgcgcag ttcgagttgg ccgccgagtt
     6121 gctgcgcccg tgcgcgcatg ttgccgatcc cgcgccctga ggcacccggc tgcgcgcttg
     6181 cgccgcggcc gtcatcctcc acttccagct cgagcaggtc gccggcctgg cgcaaccgga
     6241 cctgcacccg ggtggccgcc gcatgcttga ggacgttggt caaggcctcc tgcgcgatgc
     6301 gcagaagttg cagcgcggcg tggggcgtga ggttcagcgc gccatcgggc acgtcgatgg
     6361 tccaggccgg atggatgccg gccgccagca gctggccctg ccagcgaaac aggaagtcgc
     6421 ccagagtgga gcggaagtcg ctctcgtcgg gcgtgagcgt gtccagcgcg agccgcatgt
     6481 cggcgatgca gccgcgcagc gcatcggcaa tctgggcgcc ctgcatgtcg ccgcgctcga
     6541 cgcgcgacag cgacacgaac agctgggacc cgagcccatc gtggatctca cgcatgatca
     6601 gctggcgctc ctgtgctgcc gcatgctggc gctgcaactc cgccatctgc gcatagttgg
     6661 ccgaaagctc gcgctcgcga tcggcgacgc gcgattcgag ggtctggttc agaccctcca
     6721 gcccggtgag cgcctggatg aagcgcgcgg tcaacaggcc gcccatcgcc accagcacga
     6781 ggttcgcgcc atggtgcagc aggaagatgc gatggccggc ccagccggtc ggcagcaacg
     6841 gcacggcgcc gcccacgttc cagttcacca ggtagtcatg caggccggcg agcacggcga
     6901 gcaccatggc gaccggcatc accgccgcca tcagcgtgcg ctgccgccac accgtccaca
     6961 atgaaatggc cacgatggcg atgccgatcg gcagaaaacc ggcgatccag taccggttca
     7021 ccagcggctc ggccgcggcg ccgccggcca gcacccagag cggcccgacc agccaataca
     7081 ggagcagtcc gcgctcggcc cagggccgcc tgatgccggc gaagcgcagc gcgaggaccg
     7141 ccatggccac gatgaagccg ccggtggcgc ccaggtaggt caggcgccac agcagccagc
     7201 gctcgaccgg cagggcctcg atcacgaagg tgagggtgcg aacgccccag agtccggccg
     7261 ccaggccgaa caggccatac agcacctcat tgcggcggcg ccaccagatg aacaggacga
     7321 accccgacac cagcaggcag agcgaagcgg tgatcagcgg cgcggtgttc acccagaaga
     7381 agcgccgctc gtacagcggc gaaagctcgg cttgcggccc gatgccgggg cgcggaaacc
     7441 ccacggcggc cccgccgcgt ggcagcgccg tgcgcaccgc gacttcgttg cgcccggcgc
     7501 gcagcagcga agacggcaag gtcaccagat ggggccgcgc ccaccggatg tggacgagcc
     7561 ggctcttttg ggtcacgcgt ccggcgagcg cgccgttgag ccacacctcc ccgccatcgt
     7621 agaggtaggg caggtacagc gcccagggct cggccggctg cggtccctcg gaaaaatcga
     7681 aatccatgcg ataccaggtg tccgcgggcg gcaccccggg acgcgaccgc ctccagatgt
     7741 ccggcagggg gactgtctgc caggcccggt cgcgggcggg cggggcggca tccggcgccg
     7801 tcaacatcga gccgtgcgag ggctccagca cggcggccat catggtggcc gcggtgcacg
     7861 cgaggccaac ggccacgagg acggcacggg cccagcggcc gggccggctg gtgggccagg
     7921 acggcgtcac agcagtccca gttgcccggc ttcgtagacc gcttcgccgc gcgaatgcac
     7981 ggcgagcttg cggtagattt tcttgacgtg cgccaccacc gtatggggcg agattcccat
     8041 caactggccg atggtgtcga agctcaggcc cttggcggtc aatcgcagca gttcggtctc
     8101 acgctccgtc aacggcgagg gcgtcgcggc aggaccgggg tcggcgccgg tggcgggcgg
     8161 ggtggccacg cgaaagcgcg ccagcacgcg gcgggcgatg ccggggctga tcggcgagcc
     8221 gccggcgcgc agttcgtgaa tgctcgccgc gatgcgttcg gccaccgcgt ccttgagcag
     8281 gtagccggtc gcgcccgcct cgatggagcc gatcacgtgc tggtcgtctc cgaacatcgt
     8341 gaccacgagc acgtcgcatt gcggatggtg cgccgcggcg tagcggatca cttccacgcc
     8401 gttcatgtcc ggcaggccca gatcggccag catgacgtcg ggtcgttcgg catcgagcat
     8461 cgcctgggcc gcgcgcccgg tggacacggc tcccaccagg ttcagccccg ggtcggcgag
     8521 cacggcgttg gagaaccgcc gcatgaattc cggttcgtcc tcgacgatca gcacgctggt
     8581 cctcatggag cctccctgat gtcggtgtgg ggccctgatt ctggcactcg cgcagcctct
     8641 cgggtaaagc gcgtatgcgc aaccggcggc cggggatcag cgcactgtcg ggctgaagcc
     8701 gctagatggc gagcgggtcc acgtccacgg cccagcggat caggcccttc tcacgcgtgg
     8761 cctgcagcat cggctgccac gccgccagga agcgctgcag cgcgctgcgc gacgcgcttt
     8821 ccaccagcat ttgcgccctc tccacgttgg ccacgcgctg gatgctcatc ggcacggccg
     8881 agtagagcgt cacccgctgg gcgccgtcca gtcccgccgc ggccgcgctg gcggcccgca
     8941 ggaacgcctg cgcggcctcc tggctgcgcg cttcggcccg cagcagggcc tggaagccga
     9001 agggcggcat gccggcctgc tcgcgctcct tgagctgctg cgctgcgaag gccgggtagt
     9061 cgtgactgcg caaggccttg aacagcggat ggcgcggatg ccaggtctgc acccacatct
     9121 cgctggcctg cgcatgcgcc gcgtcgcggc cggcgcggcc ggcggcctgc atcagcaggc
     9181 tgaacaggcg ctcgggcgcg cgaaagtcgc tggagaacag ggccgagtcc gcgttgagcg
     9241 cggccaccag cgtgatgcgc cggaagtcgt ggcccttggt gaccatctgg gtgcccacca
     9301 gcacgtcgac gtcgcccgca tgcatgcggg ccagctgctg ctcgagcgcg cccttgagcc
     9361 gtgtgctgtc ggcgtccatg cgtgcaatgc gaaccgcctc gtcattggac tgcttcactt
     9421 cggcgagcaa atcggccagc tgttcctcca gccgttcggt gccgcggccc agcggcgcga
     9481 tatcgacgtt gccgcaggac gggcaggcac gcggcacacg ctccgtatag ccgcagtggt
     9541 ggcaacgcag cgtgcggtcg atcttgtgga acacccggta cgcgctgcaa tgggggcatt
     9601 cgcttttcca gtcgcaggcg gtgcaggcca gcaccggcgc gtagccgcgg cggttcagga
     9661 acaccaggga ctgctcgccg cgcgcgatgc gctgctggat ggcttcgagc agctgcggcg
     9721 agaagacggt gtgtttgggc tgcaggctca tgtccaccag ccgcacgcag ggaaacgccc
     9781 ccgaggcgcc ggcgttcgcg tccggtgcac ccgaaccccc gtcctggccc acgcgtgaag
     9841 ccatcaccag ccgccggt
//
LOCUS       DASWIY010000216         9194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2718683, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000216 DASWIY010000000
VERSION     DASWIY010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2718683"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VGA59_07910"
     CDS             <1..519
                     /locus_tag="VGA59_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9719642.1"
                     /translation="FIVVPLLAPVALTLGIDLVWFGVLLAVNMQTSFMHPPFGFALFY
                     LRSVAPEKEYTDRITRKTIAPVTTMQIYWGAVPFVLIQVVMVGLIIAFPGIVSSGLDK
                     EAVYDMDKIRIQMEKDMLKQDSLGTQPEAGVLPGAPDAGAIPMPEPSPQPAETDKELE
                     ELFKQKPQGQTR"
     gene            complement(856..2019)
                     /locus_tag="VGA59_07915"
     CDS             complement(856..2019)
                     /locus_tag="VGA59_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9719643.1"
                     /translation="MDRRLIIRGVGIAGAAALVAACGKKEEAAAPAPAAPAPAAPPAA
                     APAAPAVVTGGGAIRWRLASSFPKSLDTIFGASEVFAKQVKAMSGGKFDISVHAAGEL
                     MPAFGVVDGVQQGTIEICHTAPYYFFGKNECFALGTAIPFGLNSRQMTAWMYEGNGLK
                     LMREFYSKFDMISFPGGNTGAQMGGWYRKEIKSIKDMKGLKMRIGGFAGKVLERLGSV
                     PQNIPAGEIYQALEKGTIDAAEWVGPYDDQKLGFNKVAPYYYYPGWWEGGPQLDFYIN
                     QKAWDTLSAENKAIIESATAHAHVVMQARYDALNPTALKQLVGAGTKLRSFSPDVLSA
                     AFKESMALYSEIGTTNPDWKKIYGDYAKFRADQNLWFRFTEATFDRFMQSQKL"
     gene            complement(2238..4148)
                     /gene="dxs"
                     /locus_tag="VGA59_07920"
     CDS             complement(2238..4148)
                     /gene="dxs"
                     /locus_tag="VGA59_07920"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX9719644.1"
                     /translation="MASLLETINDPADLRKLPRPQLKVLADELRAFLLESVARTGGHL
                     SSNLGTVELTIALHYVFNTPYDRLVWDVGHQTYPHKILTGRRERMGTLRQLGGLSGFP
                     QRTESEYDAFGTAHSSTSIAAALGMAVAARQKGENRKAVAIIGDGAMSAGMAFEGLNN
                     AGVAECDLLVILNDNDMSISPPVGALNRYLAQLMSGQFYAAAKSVGKNVLKVAPPLFE
                     LAKRFEQQAKGMIVPATLFEKFGFNYIGPIDGHDLESLVPTLENIKHLQGPQFLHVVT
                     RKGQGYKLAEADPVAYHGPGKFDPAVGITKPATPPKQTFTQVFGQWLCDMAQKDLRLI
                     GITPAMREGSGLVEFQKRFPDRYFDVGIAEQHSVTFAAGLACEGLKPVVAIYSTFLQR
                     AYDQLVHDVALQNLPVVFALDRAGVVGADGATHAGAYDIAYLRCIPNVSIACPADENE
                     CRRLLTTAFEQNHPVAVRYPRGAGVGVAVEAGLHALPFGKGEVRRQRAVVPEGVPRGV
                     AILAFGTLLYPALAAAERLNATVVNMRWAKPLDADLLAQVAAQHEAIVTVEEGCIMGG
                     AGSAVAEALQAAGVLRPVLQLGLRDEFIEHGDPAKLLAAQGLDPAGIEASVRHRFAEL
                     VERVPPALKVVG"
     gene            complement(4154..5053)
                     /locus_tag="VGA59_07925"
     CDS             complement(4154..5053)
                     /locus_tag="VGA59_07925"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX9719645.1"
                     /translation="MDARVRFDLQAWGAERLDLVERALSDWVVADAPAGLGDAMRYAV
                     LDGGKRLRPMLVLAACDAVRGNEEAALRAACAVELIHAYSLVHDDMPCMDNDVLRRGK
                     PTVHVKYGQAQALLAGDALQALAFELLAPEGAAVPASVQADLCRLLARAAGHAGMAGG
                     QAIDLASVGRALGESQLREMHRRKTGALLQASVMMGAACGVPDATAQSGLAAYGAALG
                     LAFQVVDDILDVTADSATLGKTAGKDAAQDKPTYVSLLGLECSAAFARELLAQALAAL
                     AASGLRDTQALHALAIMVVDRDN"
     gene            complement(5038..5265)
                     /gene="xseB"
                     /locus_tag="VGA59_07930"
     CDS             complement(5038..5265)
                     /gene="xseB"
                     /locus_tag="VGA59_07930"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX9719646.1"
                     /translation="MSQVPFNTPVSYEAALEELDQLVGLIESGQLPLEQLLTGYQRGA
                     QLLEFCRDKLQAVENQIKVLDEGTLKPWTQE"
     gene            5515..6675
                     /locus_tag="VGA59_07935"
     CDS             5515..6675
                     /locus_tag="VGA59_07935"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX9719647.1"
                     /translation="MSDLSLQLQQAASQLPVSSYFDEALFRREMATIFQHGPRYVGHQ
                     LAVPDEGDYYALPQEGGGRALLRTPRGVELISNVCRHRQAVILKDRGSIRSGPSSGGN
                     IVCPLHRWTYSGGNRGPAGMLLGAPHFPQDPCLNLNNYPLQEWNGLLFERGPGSPDVQ
                     ADLADLGPRADLDFTGMVLDRVEMHECHYNWKTFIEVYVEDYHVGPFHPGLGSVVTCD
                     DLRWEFKQNYSVQTVGVANRLGKAGSPVYERWQNALLRYRQGKQPKYGAIWLTYYPHI
                     MVEWYPHVLTVSTLHPMGPRNTMNMVEFYYPEEIWAFEREFVEAQQAAYMETCVEDDE
                     IGERMDAGRKALMDRGDNEIGPYQSPMEDGMQHFHEWYRERIGASNALRYPV"
     gene            6752..7651
                     /locus_tag="VGA59_07940"
     CDS             6752..7651
                     /locus_tag="VGA59_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9719648.1"
                     /translation="MQALWMLLASFFFASMAVCVKIASAWFNSSEQVFYRGLLGMLFM
                     WVLARGQGVTLATRYPGMHAWRSLVGVLSLGAWFYAIAKLPLATAMTLNYMSSVWIAA
                     FLVGGALLAWNPRKGGSAPLRQGSLVLTVLCGFAGVIMMLRPTIDQHQLFAGLVGLLS
                     GISAAFAYLQVMALGKIGEPESRTVFYFAVGSVVAGGAGMAAGGVSPWDWQHALWLLP
                     VGVLASLGQLCMTRAYSHGATLVVANLQYSGIVFSGVYSVLLFDDIIPPLGWAGMALI
                     VASGIGATVLRARAAPNAPADEH"
     gene            7774..8661
                     /locus_tag="VGA59_07945"
     CDS             7774..8661
                     /locus_tag="VGA59_07945"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX9719649.1"
                     /translation="MYSVLISVDELKALQDSGAPLMIFDCSFDLMNPAAGEEQYRKAH
                     IPGAVYANVDTDLSDQGVIDPDGTHRPHPDAASGGRHPLPSREKFAMWLSSVGFTNDM
                     QAVVYDRSGATYCGRLWWMLKWAGHEAGAVLDGGLQAWQAAGGEPTDQAEPSHFQSNF
                     KLGRELRSLTTTRAIERGLGTPRQTLIDARATPRFKGEVEPLDPVAGHIPGARNRPFN
                     QNLGPDGRFKPAVQLRSEFEQLLAGRDPDTVVHHCGSGVSALPNMIAMEHAGYGPTSL
                     YAGSWSEWCGDPKRPVEKG"
     gene            complement(8688..9077)
                     /locus_tag="VGA59_07950"
     CDS             complement(8688..9077)
                     /locus_tag="VGA59_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719650.1"
                     /translation="MTPRALLLAALLSVGLAGCERTSEGIPDAQGSRGTGNPREVVQP
                     SAPDTPPRGSAGDPVPGATGSGTSDAGGRSQTPQSGTGLAGGMGGPSSLGMTGTFPSG
                     TATQSQGEGEAAAGSGNRTPAGGVGMR"
BASE COUNT         1517 a         3102 c         2984 g         1591 t
ORIGIN      
        1 ttcatcgtgg tgccgctgct ggcgcccgtg gccttgacgc tcggcatcga cctggtctgg
       61 ttcggtgtgc tgctggccgt gaacatgcag acgtcgttca tgcacccgcc gttcggattc
      121 gccctgtttt acctgcgcag cgtcgcgccg gaaaaggaat acaccgatcg cattacgagg
      181 aagacgatcg cgccggtgac caccatgcag atctactggg gcgcggtgcc gttcgtgctg
      241 atccaggtcg tcatggtcgg cctgatcatc gccttccccg gcattgtctc cagcgggctg
      301 gacaaggaag cggtctacga catggacaag atccgcatcc agatggaaaa ggacatgctc
      361 aagcaggact cgctcggcac ccagcccgag gcgggtgtgc tgccgggcgc gcccgatgcc
      421 ggcgccatac ccatgcccga gccctcgcct cagccagcgg agaccgacaa ggagctcgag
      481 gaactgttca agcagaaacc gcaaggccag acccggtagc ctcgccgccg gggcatggat
      541 tgcgggtcat gcccgcaatg acaacagcag ttgtgtctcg gctgtcatcc cggccccgga
      601 tcaagtccgg ggtttcagca cccgggatcc atggctcgaa ggaagtggtg tgccgccatg
      661 gattgcgggg ccgggccgga gcggccatgc ctcggtacgt gcatagcgca ggcccggcat
      721 tgggctcgct gcactggaca gctgcgcgcc ccgggcgttc cgcgagccca gcccgcaatg
      781 acatcaaaga aaaagggccc gcgagggccc ttccacaagg acaaggcgca caccgtgcgc
      841 cttgttatcg tccgtttata gcttctgcga ttgcatgaaa cggtcgaagg tggcttcggt
      901 gaagcggaac cacaggttct ggtcggcgcg gaacttggcg tagtcgccgt agatcttctt
      961 ccagtcgggg ttcgtcgtgc cgatttcgct atacagggcc atggattcct tgaaggccgc
     1021 ggacaggacg tccggcgaga acgagcgcag tttcgtgccg gcacccacca gctgcttcag
     1081 cgccgtgggg ttgagcgcgt cgtacctggc ttgcataacc acgtgggcat gggccgtggc
     1141 cgactcgatg atcgccttgt tttcggcgga cagcgtgtcc cacgccttct ggttgatgta
     1201 gaagtcgagc tgcggcccgc cttcccacca gccagggtag taatagtagg gcgcgacctt
     1261 gttgaagccc agcttctggt cgtcgtaggg tcccacccac tcggccgcat cgatcgtgcc
     1321 tttttccagg gcttgataga tctcgccggc ggggatgttc tgcggcacgc tgcccaggcg
     1381 ctcgagcacc ttgccggcaa agcctccgat tcgcatcttc aggcccttca tgtccttgat
     1441 cgacttgatt tccttgcggt accagccacc catctgcgca ccggtgttgc cgcccgggaa
     1501 gctgatcatg tcgaacttgg agtagaactc gcgcatcagc ttcaagccgt tgccttcgta
     1561 catccaggcc gtcatctggc ggctgttcag gccaaacggg atggcggtgc cgagcgcgaa
     1621 gcattcgttc ttgccgaaga aatagtaagg ggcggtgtgg cagatttcga tggtgccttg
     1681 ctgcacgcca tcgaccacac cgaaagcggg catcagctcg ccggcggcat gcaccgagat
     1741 gtcgaacttg ccgccggaca tggccttgac ctgcttggcg aaaacttccg aggcgccgaa
     1801 gatggtgtcc agcgatttcg ggaagctcga cgcgaggcgc cagcggatgg cgccgccacc
     1861 ggtcacgacg gccggtgccg cgggcgccgc ggcgggcgga gccgccggag cgggagcagc
     1921 cggcgcgggc gcagcggcct cttctttctt gccgcatgcc gcgaccaggg cagcagcccc
     1981 ggcgattccg acacccctga tgattagccg acggtccata tgactcactc ctcagtttgg
     2041 aaaaataatg aaaggcggta ttgatccggc ccacacgaac gttgagcaac gtgccgccat
     2101 tctaggaacg cacccacttt ggtattcagg ggttttccct tgggaagaac cccgaaccct
     2161 ttggtactca gggttctgtc aggaaagcta cagcgaggaa cgcgaacggt tgcctcggct
     2221 tcggccccgt aaaaccttta accgacgacc ttcaacgcgg gcggcacgcg ttcaacgagc
     2281 tccgcgaagc gatggcgtac cgaagcctcg atgcccgccg ggtccagccc ctgcgccgcc
     2341 agcaacttgg ccgggtcgcc gtgctcgatg aattcgtccc gcagccccag ctgcagcacg
     2401 ggccgcagca cgccggccgc ctgcagtgcc tccgccaccg cgctgcccgc gccgcccatg
     2461 atgcagcctt cctccaccgt cacgattgcc tcgtgctgcg ccgccacttg cgccagcaag
     2521 tcggcgtcca gcggcttggc ccaacgcatg ttcaccaccg tcgcattgag gcgttcggcc
     2581 gcggccagcg cggggtagag cagcgtgccg aaagccagga tggccacgcc gcggggaacg
     2641 ccctcgggca caaccgccct ctgccgccgc acctcgccct tgccgaacgg caaggcgtgc
     2701 aagcccgctt cgaccgccac acccaccccg gcgccgcgcg gatagcgcac cgcgacggga
     2761 tggttctgct cgaaggccgt ggtcagcagg cggcggcatt cgttctcgtc ggcggggcag
     2821 gcgatgctca cgttcggaat gcaacgcagg tatgcgatgt cgtaagcacc cgcatgcgtg
     2881 gcgccgtcgg cgcccaccac gcccgcgcgg tccagcgcga agaccacggg aagattctgc
     2941 agcgccacgt cgtgcaccag ctggtcatag gcacgttgca ggaaggtgga gtagatggcc
     3001 acgaccggtt tcaggccttc gcacgcgagg cccgccgcga acgtgaccga atgctgctcg
     3061 gcgatgccca cgtcgaagta gcgatcgggg aaacgctttt ggaactccac cagccccgag
     3121 ccctcgcgca tcgcgggggt gatcccgatc aggcgcaaat ccttttgcgc catgtcgcac
     3181 agccactggc cgaacacctg ggtgaacgtc tgcttgggcg gggtcgccgg cttggtgatg
     3241 cccaccgccg ggtcgaactt gcccggcccg tggtaggcca ccgggtcggc ttccgccagc
     3301 ttgtagcctt ggcccttgcg agtgaccaca tgcaggaatt gcggcccttg caggtgcttg
     3361 atgttctcca gcgtgggcac cagagactcc aggtcgtggc cgtcgatcgg cccgatgtag
     3421 ttgaagccga acttctcgaa cagcgtggcc ggtacgatca tgcccttggc ctgctgctcg
     3481 aagcgcttgg ccagctcgaa caggggcggc gcgaccttga gcacgttctt gccaacgctc
     3541 ttggcggcgg catagaactg gccgctcatg agctgcgcca ggtagcggtt cagcgcgcct
     3601 accggcgggc tgatcgacat gtcgttgtcg ttcaggatca ccagcaggtc gcattcggcg
     3661 acaccggcat tgttcagccc ttcgaaggcc atgcccgcgc tcatggcgcc gtcgccgatg
     3721 atggccacgg ccttgcggtt ctcccctttt tgccgggccg ccaccgccat gccgagagcg
     3781 gcggcaatgc tggtggacga atgcgccgtg ccgaaggcgt cgtattcgct ttcggtgcgc
     3841 tgggggaatc ccgacaggcc gcccagctgg cgcaaagtgc ccatgcgctc gcgccgcccc
     3901 gtgagaatct tgtgcggata ggtctggtgg cccacgtccc acaccagccg gtcgtaaggg
     3961 gtgttgaaaa cgtaatgcag ggcgatcgtc aattccaccg tgcccaggtt ggaactgaga
     4021 tggcccccgg tgcgggccac gctctccagc aggaaagccc gcagctcatc cgccagcacc
     4081 ttcagctgcg ggcgcggcag cttgcgcagg tccgcggggt cgttgatcgt ttcgagcaag
     4141 gaggccatcg gtttcaattg tccctgtcga ccaccatgat ggccaatgcg tgtaaagcct
     4201 gtgtgtcccg caagccgctc gcggccaatg ccgccagcgc ctgggccagc agctcgcgcg
     4261 cgaacgcggc cgagcattcc agaccgagca gcgacacgta ggtcggcttg tcctgcgcgg
     4321 cgtccttgcc cgccgtcttg ccgagcgtgg ccgagtcggc cgtcacgtcc aggatgtcgt
     4381 cgaccacctg gaaagccagg cccaaggccg caccataggc ggccaggccc gactgggccg
     4441 tcgcgtccgg gacgccacag gccgcgccca tcatgacact ggcctgcagc agcgcgccgg
     4501 tcttgcggcg gtgcatctcg cgcagctggc tttcgccgag cgcacggccg acgctcgcca
     4561 ggtcgatcgc ctggcccccg gccatccccg cgtgtccggc cgcgcgcgcc agcagccggc
     4621 acaagtccgc ctgcaccgat gcgggcacgg ccgcgccttc cggtgcgagc agctcgaagg
     4681 ccagtgcctg caaggcatcg cccgccagca aggcctgggc ctggccgtat ttcacatgca
     4741 ccgtcggttt accgcggcgc aggacgtcat tgtccatgca cggcatgtcg tcgtggacca
     4801 gcgagtaggc atgaatgagc tcgacggcgc aggccgcgcg cagtgcggcc tcctcgttgc
     4861 cccgcaccgc gtcgcaggcg gccagcacca gcatcggccg cagccgcttg ccgccgtcca
     4921 gcacggcgta acgcatcgcg tcgcccaggc ccgccggcgc atccgccacc acccagtccg
     4981 acagcgcccg ttcgaccaga tccagccgct cggcgcccca ggcctgcaga tcgaacctca
     5041 ctcttgcgtc cacggcttca gcgtgccttc gtccagcacc ttgatctggt tttcaacggc
     5101 ctgcagcttg tcgcggcaaa actccagcag ctgggcgcca cgctggtatc ccgtcagcag
     5161 ctgctcaagg ggcagctgcc ccgactcgat caggccgacg agctggtcca gctcttccag
     5221 cgcagcttca tagctgaccg gggtgttgaa agggacctgg gacatgatca aattgcgtta
     5281 gcggaaaacc ctcaattcta ggcggtgcgg ccctgctgct ccgcaggagg cctccccaat
     5341 cgcagggcgt gcggacttga tacccttccg gggacccagt tgggaccccg ggggtacaat
     5401 cgccttccct tcacgcgggc accgcccgct tccccaactc ccttcccgcg ccaatccacg
     5461 gaaaaagcgc ttaccctgcc ccttcatagg gggagtctct aggtcaggtc acccatgtct
     5521 gatttaagtc ttcaactgca gcaggccgca agccaactac cagtttccag ttacttcgat
     5581 gaggcgctgt tccggcgcga gatggcaacc atcttccagc acggaccccg ctatgtgggg
     5641 caccagctcg cggtgccgga cgagggcgac tactacgccc tgccccagga gggcggcggg
     5701 cgcgcgctgc tgcgcacgcc gcgtggcgtg gaattgattt ccaacgtctg ccgccatcgc
     5761 caggccgtga tcctgaagga ccgcggatcg atccggtccg gcccctcctc gggcggcaac
     5821 atcgtatgcc cgctgcaccg ctggacctac agtggcggca accgcggccc ggccggcatg
     5881 ctgctgggtg ctccgcattt cccgcaggac ccttgcctga acctcaacaa ctatccattg
     5941 caggaatgga atggactgct gttcgaacgg ggtccgggaa gccccgacgt ccaggcggat
     6001 ctggcggact tgggcccgcg cgccgacctc gatttcacgg gcatggtgct cgaccgcgtg
     6061 gaaatgcacg agtgccatta caactggaag acgttcatcg aggtttatgt ggaggactac
     6121 cacgtgggcc cctttcatcc cggcctgggc agcgtggtca cctgcgacga cctgcgctgg
     6181 gagttcaagc agaactattc ggtgcagacc gtgggcgtgg ccaaccggct gggcaaggcc
     6241 ggcagtccgg tctacgagcg ctggcagaac gcgctgctgc ggtaccgcca gggcaagcag
     6301 cccaagtacg gcgcgatctg gctcacctac tacccgcaca tcatggtcga gtggtacccg
     6361 cacgtcctca ccgtttccac gctgcacccg atggggcctc ggaacaccat gaacatggtg
     6421 gagttctact accccgagga gatctgggcg ttcgagcgcg agttcgtcga agcgcaacag
     6481 gctgcctaca tggaaacctg cgtggaggac gacgagatcg gcgagcgcat ggacgcgggc
     6541 cgcaaggcgc tgatggaccg cggcgacaac gagatcggcc cctaccagag ccccatggaa
     6601 gacggcatgc agcacttcca cgagtggtat cgcgagcgca taggcgccag caacgctttg
     6661 cgctatccgg tctgatgccc tcgtttccgg gtggccggca cagcgcaacg gtggtcccgc
     6721 tggaccgcaa gattctctag ccgctccctc gatgcaagcg ctgtggatgc tcctggcttc
     6781 gttcttcttc gcgtccatgg cggtgtgcgt gaagatcgcc tcggcctggt tcaacagctc
     6841 cgaacaggtg ttctaccgcg gcctgctggg catgctcttc atgtgggtgc tggcgcgcgg
     6901 gcaaggcgtc acgctggcca cccgctatcc cggcatgcac gcctggcggt ccctggtggg
     6961 cgtgctgtct ctgggggcct ggttctacgc catcgccaag ctgccgctgg ccacggcaat
     7021 gacgctgaac tacatgagca gcgtctggat cgcggccttc ctggtcggcg gcgccttgct
     7081 cgcatggaat ccgcgcaaag gcggcagcgc accgctgcgg cagggctcgc tggtactcac
     7141 ggtgctctgc ggcttcgccg gtgtgatcat gatgctgcgg ccgacgatcg accagcacca
     7201 gctgttcgcg ggattggtgg gactgctgtc gggaatctcg gcggccttcg cctacctgca
     7261 ggtgatggcg ctgggcaaga tcggcgagcc ggaatcgcgg acggtgttct atttcgcggt
     7321 cggttcggtc gttgccggcg gcgccggaat ggcggcgggc ggcgtgtcgc cctgggactg
     7381 gcagcacgcg ctgtggctgc tgccggtggg cgtgctggca tcgctggggc agctgtgcat
     7441 gacccgcgct tacagccatg gggccaccct ggtcgtcgcg aacctgcaat attccggcat
     7501 tgtgttctcg ggcgtgtaca gcgtgcttct gttcgatgac atcatcccgc cactgggctg
     7561 ggccggcatg gctctgatcg tcgccagcgg tatcggggca acggtgttgc gcgcccgcgc
     7621 cgcgccgaac gcgccggcgg acgagcattg aaactggcat tgcggacgcg catcaaggct
     7681 gtcattgcgg actcgatccg caatccatgg tgattcgcaa cataatccgc tatcgattca
     7741 tggatgccgg atcgagtccg gcatgacaac tttatgtaca gtgttctcat ctccgtggac
     7801 gagctcaagg ccctgcagga cagcggtgcg ccgctgatga tcttcgactg cagcttcgac
     7861 ctgatgaatc cggccgccgg cgaagagcaa taccgcaagg cgcacattcc cggcgcggtc
     7921 tatgcgaacg tcgacacgga cctgagcgat cagggcgtga tcgatcccga cggcacacac
     7981 cgcccgcacc ccgacgcagc ttcgggcggc cggcatccct tgcccagccg cgagaaattc
     8041 gcgatgtggc tgtccagcgt cggcttcacc aatgacatgc aggccgtggt gtacgaccgc
     8101 agcggcgcca cctactgcgg gcggctctgg tggatgctga aatgggccgg ccacgaggcc
     8161 ggggccgtgc tcgacggcgg tctgcaggct tggcaggcgg caggcggcga gccgacggac
     8221 caggcggagc catcgcactt ccagtccaac ttcaaactcg gccgcgagct gcgctcgctg
     8281 accaccacgc gcgcaatcga gcgcggtttg ggcaccccgc gccagacgct gatcgacgcc
     8341 cgcgccacgc cgcgtttcaa gggtgaggtg gaaccgctgg acccggtggc gggccatatc
     8401 cccggtgcgc ggaaccgacc cttcaaccag aacctcggac ccgacggcag gttcaaaccc
     8461 gcggtccagc tcaggtcgga attcgagcaa ctgctggcgg gccgcgatcc ggataccgtg
     8521 gtgcaccact gcggcagcgg cgtgagcgca ctgcccaaca tgattgcgat ggagcacgcc
     8581 ggttacggcc ccacatccct ctacgcgggc agctggagcg agtggtgcgg cgacccgaag
     8641 cggccggtcg agaaaggctg acgcaagccg gggcagcccg cggctaacta acgcatgccc
     8701 acgcctccag ccggtgtccg gttcccactg cccgcggcgg cctcgccttc accctgtgat
     8761 tgcgtcgcgg tgccgctggg gaaggttccg gtcatgccca ggctcgacgg gccgcccatg
     8821 ccgcccgcca gccccgtccc ggactgcggt gtctggctgc gcccacccgc atcggaggtt
     8881 ccactgcccg tggcgccggg cacggggtcg ccggccgaac cgcgcggcgg tgtgtcgggt
     8941 gcggacggct ggaccacctc gcgcgggttg cctgtgcccc ggctgccctg cgcatccgga
     9001 atgccctcgc ttgtgcgctc gcagcccgcc aaccccaccg acagcaacgc cgccagtagc
     9061 agggcgcgtg gcgtcacgtc agttgctcga ccgcgctgcg cgcgatccgc ggcgtatgaa
     9121 atcgggcggt cagtgcgggg gccaccgcgg aaccggcttt gccgggccgc tggtggcgcc
     9181 cccttgaggg ggag
//
LOCUS       DASWIY010000217         4315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_863937, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000217 DASWIY010000000
VERSION     DASWIY010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863937"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VGA59_07955"
     CDS             complement(<1..366)
                     /locus_tag="VGA59_07955"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX9719651.1"
                     /translation="MGLDSFRPAQELNMIGARDSGSLSRLRERARVRARALRQSQTDA
                     EALLWSKLRARQMQGLKFRRQHPLGNYFADFACIEIGLIVELDGGQHGEALAIAYDSK
                     RTDKFAAMGFQVLRFWDNEV"
     gene            complement(330..833)
                     /locus_tag="VGA59_07960"
     CDS             complement(330..833)
                     /locus_tag="VGA59_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEX9719652.1"
                     /translation="MNHQPNRMQRQLGRLNEVPQFLRPWAQSMVLRRAVPFTGTAGLD
                     FVTLTPSQVEIAIPNHKRVQNHIGGVHASAMNLLAETATGMVVGMNVRDDCLPLAKEL
                     KMAFRKRATGSLRAVATLTDAQRAAMQGSDKGEVKVAVTVTDETGVNPVECEFIWAWI
                     PSGPRRS"
     gene            complement(835..3234)
                     /locus_tag="VGA59_07965"
     CDS             complement(835..3234)
                     /locus_tag="VGA59_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HEX9719653.1"
                     /translation="MNRFQVKKVAVLGAGVMGAQIAAHLVNVKVPVVLFDLPAKDGPK
                     NAIVSKAVDGLKKLKPSPLGVKDEAALIEQANYEEHMERLKDCDLIIEAIAERMDWKL
                     DLYRKIAPFVAPHAILASNTSGLSITKLSEALPEEMKPRFCGIHFFNPPRYMVLVELI
                     NTPTTDAHILDDLETFVTSGLGKGVVRAKDTPNFIANRVGIAGMLSTMREVEKFGLTY
                     DVVDDLTGKKMGRASSGTFRTADIVGLDTMAHVIKTLQDTLNAQTDPFYASFETPQVL
                     KTLLEIGNLGQKTKAGFYKKAGRDVMRFDLATKEYVPAGEKADEVYGRMLKKPPAERL
                     KLLRNAEGAQGQFLWAILRNSFHYAAVHLATIAETARDVDFALRWGFGMKQGPFELWQ
                     EAGWLQVAQWIQEDIAAGKALSSAPLPDWVFKGPVAQAGGVHTPEGSWNPTTGRFEPR
                     RRLPVHARQHFPEAVLGSNAPRFETAGKTLHEDHAIRLWTLDDDVVIASIKTRMHAIS
                     PEVSEGLQMAIELAEKKYQGLVIWSGDDPFSVGADLQAMLPAFIAVGVSAIDDAEQFM
                     QQTMLRLRYANVPVIAAIRGMALGGGCEIAVHSARRVVAMESYIGLVEVGVGLVPGAG
                     GLTYIARRAAEAQARSTHKDILPFLTEGFIAAAMAKVGTSALESRQLGYLLDSDVIVP
                     NKDELLFVALNEAKAMFTAGYRPPLKRQFAVAGRNGKATIQGQLVNMRDGGFASAHDF
                     HIASLIANVVTGGDVDTGTLVTEEYLMALERQAFCALVQHPKTQERILGMLSTGKPVR
                     N"
     gene            complement(3451..>4315)
                     /locus_tag="VGA59_07970"
     CDS             complement(3451..>4315)
                     /locus_tag="VGA59_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9719654.1"
                     /translation="AYQNSLAYAKERLQSRSLTGPKAANKPADPIIVHPDVRRMLMTI
                     RSFTEGARALALWVGLHIDLAAKHPDERQRRDADDLAALLTPVIKGYLTDVGFESTVM
                     AMQCYGGHGYIHEWGMEQFVRDARINMIYEGTNTIQSLDLLGRKVLGDNGKRLKAFGK
                     LVGQLVEEEGVNEKMAEFINPIAYLGDQMTKFTTELGFKGFQNPDEVGAAAVDYLRVL
                     GHLVHGYLWARMAQVALREIAAGNTDPFYQAKLQTARFYFAKLFPETATLMRTARAGS
                     GVLMDTDLALA"
BASE COUNT          684 a         1436 c         1356 g          839 t
ORIGIN      
        1 cacttcgttg tcccaaaagc gcaagacttg aaatcccatg gccgcgaatt tgtccgtgcg
       61 cttgctgtca tacgcgatcg ccaatgcctc gccatgctgg ccgccgtcca gttcgacgat
      121 caagccaatt tcaatgcagg cgaaatcggc gaaatagttg cccagcggat gctggcggcg
      181 gaatttgaga ccctgcatct gccgtgcccg aagcttggac cacaacaagg cttccgcgtc
      241 cgtctggctt tgtcgcagcg cccgcgccct caccctagcc ctctcccgca agcgggagag
      301 ggaaccggag tcccgggcgc cgatcatgtt cagctcctgc gcgggccgga aggaatccag
      361 gcccatatga attcacattc gaccgggttg acgccggtct cgtcggtcac cgtgaccgcc
      421 actttcacct cgcccttgtc gctgccctgc atcgcggcac gctgcgcgtc cgtgagcgtg
      481 gcgacggcgc gcagcgatcc ggtggcccgc ttgcggaacg ccatcttcag ctccttggcc
      541 agcggcaggc agtcgtcgcg cacgttcatg cccacgacca tgcccgtggc cgtctcggcc
      601 agcagattca tcgccgaagc gtggacgccg ccgatgtggt tctggacccg cttgtgattg
      661 ggaatcgcta tttccacctg gctgggtgtc aaggtgacga agtccagtcc ggctgtcccg
      721 gtgaacggca cggcgcggcg cagcaccatg ctctgggccc aggggcgcag gaactgcggc
      781 acttcgttca gccggcccag ctggcgctgc atccggttgg gttgatgatt catgtcaatt
      841 acggacgggt tttccagtgg agagcatgcc caggattcgc tcctgcgtct tcggatgctg
      901 caccagcgcg cagaacgcct ggcgctccag cgccatcaga tattcctcgg tcaccagcgt
      961 gccggtgtcc acgtcgccac cggtcaccac gttggcgatg agcgaagcga tatggaagtc
     1021 gtgcgcgctg gcgaagccgc cgtcgcgcat gttcaccagc tggccctgga tggtggcctt
     1081 gccgttgcgg cccgccaccg cgaactggcg cttgagcggc ggacggtagc cggcggtgaa
     1141 catcgccttg gcttcattga gcgccacgaa cagcaactcg tccttgttcg gcacgatgac
     1201 gtcgctgtcc agcagatagc cgagctggcg cgactccaac gcgctcgtac ccaccttcgc
     1261 catggccgcg gcgatgaagc cttcggtgag gaagggcagg atgtccttgt gcgtggagcg
     1321 cgcctgggcc tcagcggccc ggcgcgcgat gtaagtcaag ccgccggcgc cggggaccag
     1381 ccccacgccg acttcaacca acccgatgta gctctccatc gcgaccaccc gccgtgccga
     1441 gtgcacggcg atttcgcagc cgccgcccag cgccatgcct cgaatggcgg cgatcacggg
     1501 cacgttggca taacgcaggc gcagcatggt ctgctgcatg aactgctcgg catcgtcgat
     1561 ggcgctcacc cccacggcga tgaaggcggg cagcatggct tgcaggtcgg cgccgacgct
     1621 gaagggatcg tcgcccgacc agatcaccag cccctggtat ttcttctcgg ccagctcgat
     1681 cgccatctgc aggccctcgg atacttccgg actgatggca tgcatcctgg tcttgatgct
     1741 ggcgataacc acgtcgtcat cgagcgtcca aagccggatc gcatggtcct cgtgcagcgt
     1801 cttgccggcg gtctcgaacc gcggtgcgtt gctgcccagc accgcctccg ggaaatgctg
     1861 gcgcgcatgg accggcagcc gacggcgcgg ctcgaacctt ccggtggtcg ggttccagga
     1921 gccctcgggc gtgtgcacgc cgccggcctg cgccaccggg cccttgaaca cccagtcggg
     1981 aagcggagcg ctggacagtg ccttgccggc ggcgatgtct tcctggatcc actgcgccac
     2041 ctgcaaccag ccggcttcct gccacagctc gaagggcccc tgcttcatgc cgaaacccca
     2101 gcgcaacgcg aagtccacgt cacgcgcggt ttccgcgatg gtggccaggt gcacggccgc
     2161 gtaatggaag ctgttgcgca ggatcgccca caggaactgg ccctgcgcgc cttcggcgtt
     2221 gcgcagcagc ttcaggcgct cggccggcgg cttcttcagc atgcggccat agacctcgtc
     2281 cgccttttca ccggcgggga cgtactcctt ggtcgccagg tcgaagcgca tcacgtcgcg
     2341 acccgccttc ttgtagaagc cggccttggt cttctggccg agattcccga tttccagcag
     2401 cgtcttgagt acctggggcg tttcgaagct cgcatagaac ggatcggtct gcgcgttcag
     2461 tgtgtcctgc agcgtcttga tcacatgggc catggtgtcc aggcccacga tgtcggcggt
     2521 gcggaaggtg cccgagctgg cccggcccat cttcttgccg gtcaggtcgt cgaccacgtc
     2581 gtaggtcagg ccgaatttct ccacctcgcg catggtcgac agcatgccgg cgatgccgac
     2641 gcggttcgcg atgaagttgg gcgtatcctt cgcacgcacc acgcctttgc cgagcccgct
     2701 ggtgacgaat gtctccaggt cgtccaggat atgcgcatcg gtggtgggcg tgttgatcaa
     2761 ctccaccagg accatatagc ggggcgggtt gaagaagtgg atgccgcaga agcgcggctt
     2821 catttcttcc ggcaatgctt cactgagctt ggtgatcgac agccctgacg tgttggaagc
     2881 aaggatcgca tgcggcgcca cgaacggcgc gatcttgcgg tacaggtcca gcttccagtc
     2941 catccgctcg gcgatggcct cgatgatcag gtcgcagtcc ttcaaccgct ccatgtgctc
     3001 ttcgtagttc gcctgctcga tgagcgccgc ctcgtccttc acgcccaggg gagagggctt
     3061 gagcttcttg aggccatcaa ctgccttcga aacaattgca ttcttcggcc catctttggc
     3121 cggcaggtcg aacagcacca ccggaacctt gacgttgacc aggtgcgcgg cgatctgcgc
     3181 gcccatcacg cccgcgccga gcacggcgac tttcttcacc tggaagcggt tcatggtctt
     3241 cggcccttca ctgttggcgc cgggttcggc cccgccgcgc gcttcgtgtt ctttgtacat
     3301 ggtattggtg tccaagatat tttttgggct gtcatcccgg gctggacccc ggatcaggtc
     3361 cggggcaggc tccgggatcc atgctgcgct ggctattcgc cgactggact gcatggattg
     3421 cgggtcgagc ccgcaatgac agattcgtta tcaggccagg gccaggtccg tgtccatcaa
     3481 cacaccgctg cccgcgcgcg ccgtgcgcat cagcgtggcg gtttccggga acagcttggc
     3541 gaagtagaaa cgcgccgtct gcagcttggc ctggtagaac ggatcggtat tgccggccgc
     3601 gatctcgcgc aacgcgacct gcgccatgcg cgcccacaaa tagccgtgca ccaggtgccc
     3661 cagcacccgc aggtaatcga cggcggcggc gccgacctcg tccggattct ggaatccctt
     3721 gaagccgagt tcggtcgtga acttggtcat ctgatcgccc aggtaggcga tcgggttgat
     3781 gaactccgcc atcttctcgt tcacgccttc ctcttcgacc agttgcccca ccagcttgcc
     3841 gaaggccttc aggcgcttgc cgttgtcgcc cagcaccttg cggcccagca ggtccagcga
     3901 ctggatcgtg ttcgtgccct cgtagatcat gttgatgcgc gcgtcgcgca cgaactgctc
     3961 catgccccac tcgtggatgt agccgtgccc gccgtagcac tgcatcgcca tcaccgtgct
     4021 ctcgaagccg acgtcggtga ggtagccctt gatcaccggc gtcagcagcg ccgccaggtc
     4081 gtcggcgtcg cggcgctggc gctcgtcggg atgcttggcc gcgaggtcga tatgcaggcc
     4141 gacccatagc gccagggcgc gggcgccctc ggtgaacgac ctgatggtca tcagcatgcg
     4201 ccgcacgtcg gggtgcacga tgatcgggtc ggccggcttg ttggcggcct tcggtccggt
     4261 gagcgagcgg ctttgcaggc gctccttcgc ataggccagc gagttctggt acgcg
//
LOCUS       DASWIY010000218         7684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_864467, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000218 DASWIY010000000
VERSION     DASWIY010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864467"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1309
                     /locus_tag="VGA59_07975"
     CDS             335..1309
                     /locus_tag="VGA59_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9719655.1"
                     /translation="MSLLQVNNLIVEFPGRRGTLRALDDISFDIAPGEILGVVGESGA
                     GKSLTGASIIGLLEPPGRVVSGQILLEGRRIDNLPPEQMRHIRGRKIGAIFQDPLTSL
                     NPLYTVGRQLTETIQAHLPVNIGEARKRAVGLLEDTGIPAAAERIDHFPHQFSGGMRQ
                     RVVIALALAAEPKLIVADEPTTALDVSIQAQIIQLLKRVCKDRGAAVMLITHDMGVIA
                     ETCDRVAVMYAGRIAEIGPVHEVINQPAHPYSMGLMAAIPDITIERERLNQIDGAMPR
                     LDAIPPGCAYNPRCPRAFDRCIAERPDLLEAGATRAACWLHDAHAGVT"
     gene            1419..2414
                     /locus_tag="VGA59_07980"
     CDS             1419..2414
                     /locus_tag="VGA59_07980"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX9719656.1"
                     /translation="MNAPLVQAHDLAKTFDVSVPWLNRVIERRPRQLLKAVDGVSFEI
                     GKGKTLALVGESGCGKSTVARLLVGLYEPTRGGLTFDGQNAHAAFKAPQGRQLRRRIQ
                     MIFQDPYASLNPRWIVEDIVGEPLKEHGIITDAAQLEVRVGELLESVGLSPLDMAKYP
                     HQFSGGQRQRISIARALATEPEFLVCDEPTSALDVSVQAQVLNIMKDLQRQRGLTYLF
                     ISHNLAVVRHVSDQVGVMYLGRIVELADKRELFGNPRHPYTRMLLDAIPKMHDTGRRR
                     TPVQGEVPNPLNPPTGCAFHPRCPHANARCKAERPALLEIRGVKIACHAVEEGRL"
     gene            complement(2653..3498)
                     /locus_tag="VGA59_07985"
     CDS             complement(2653..3498)
                     /locus_tag="VGA59_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX9719657.1"
                     /translation="MLDRIKASLPSLAPAEQRVGRLVLSDPRAFANLPVSELADRAHV
                     SKPTVVRFCRSIGYDGLSDFKLKLAGSVSEGVPFIHRSVDADDKTGDVLVKVIDNTVA
                     AFLKYRNDASTFAVEKAVEALASTCKTGRRIEFYGVGNSGIVAQDAQHKFFRLGVNTI
                     AYSDGHMQVMSATLLGPGDCVVVISNSGRTRDLMDACDIARKNGATTIVITATGSPLA
                     SAGQIHLAADHPEGYDRYSPMVSRLLHLVIIDVLATAVALRIGPRLQPLLREMKNNLR
                     NKRYA"
     gene            complement(3575..5041)
                     /gene="zwf"
                     /locus_tag="VGA59_07990"
     CDS             complement(3575..5041)
                     /gene="zwf"
                     /locus_tag="VGA59_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX9719658.1"
                     /translation="MSFDLVLFGGTGDLAWRKLMPALFQAFRHGSLPEGARIIGVARD
                     NLDDGQYRSLIRSRFDQVELAKRPSPGEFERFAALLRFLRMDLSQPGDYQRLAATLRE
                     RQAATIVMYLATAPSLFTVVCEQLAAAGLNTANTRLVLEKPLGHDLASNRAINACVRR
                     VLGEKQIFRIDHYLGKPSVQNLFALRFGNALFEPLWRRETIANIQITLAEDLGTENRG
                     AFYDSTGALRDMVQNHALQLLCALAMEPPINAHADAIRDEKLKVLRSLKPWTQQSVAQ
                     HVVRGRYMAGRVAGETVPGYLDEPGVDPQSTTETFVALRAEIANWRWAGVPFYIRTGK
                     RLAAADARIVVNFQPAPHAIFHSPNGAANRLVIKLQPNDGLELHLLAQGPENRQRSRS
                     AGQSLTQVQLDLDFDKRFGSERVGAYERLLLDVIDGRLNLFVRSDEQEEAWRWVEPIM
                     EYWDGETSGPRPYAAGTWGPSAASAMIARDGHCWSEEC"
     gene            5281..5379
                     /locus_tag="VGA59_07995"
                     /pseudo
     CDS             5281..5379
                     /locus_tag="VGA59_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834007.1"
                     /note="maintains the balance of metabolites in the
                     pentose-phosphate pathway; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
     gene            5383..6966
                     /gene="pgi"
                     /locus_tag="VGA59_08000"
     CDS             5383..6966
                     /gene="pgi"
                     /locus_tag="VGA59_08000"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX9719659.1"
                     /translation="MAASITRPRCDETTAWAALQEHFAAIGRTLDLRDAFRCDPGRFE
                     TFSQEAPHVFADLSKNLIDTRAEALLLELARACGLEGHRDAMFAGHAINSTEQRAVKH
                     WLLRAPGGAGTDPDAAQVHATLDAMLAYAEQVRADEAITDVVNIGIGGSDLGPQMAVA
                     ALDAFVLPGKRLHFVSNIDGHELAAVLRRVRPESTLFLVASKTFTTIETMTNARSART
                     WFEAEGGRDPGSGPGAGVARHFAALTTNVAAAREFGIATTFGFWDWVGGRYSMWSAIG
                     LPIAIAVGAAGFRELLAGAHEMDRHFATEPLARNLPVRLGLLDVWNRNFHGFASRSIA
                     PYHSALRRLPAYLQQLEMESNGKRVDAGGRPLSQASAPVLWGEPGTNGQHAYFQMLHQ
                     GTDVIPVEFIAVKTPAHGLAGHHELLLANALAQAQALMQGKPDAGGHRDFPGNRPSTF
                     FLLERLTPASLGALLALHEHRVFVSGSIWGINSFDQWGVELGKVLALDIAPRLASGDA
                     SGLDASTAGLLHRLGTPPA"
     gene            7039..7404
                     /locus_tag="VGA59_08005"
     CDS             7039..7404
                     /locus_tag="VGA59_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEX9719660.1"
                     /translation="MNFSQAVATCLRKYVDFSGRAARPEFWWFFLFQVIVMAVTGMMS
                     DILNGIAALALLLPGLSVGARRLHDIGKSAWFLLLGLIPILGFLVLLYWFVQPSAPAN
                     AYGPAESGSDAPTVMPGQQ"
BASE COUNT         1222 a         2609 c         2584 g         1269 t
ORIGIN      
        1 aaggagatgt cgtccagcgc gcgcaggatg ccgcggcgcg tcgggaactc gacgatgagg
       61 ttcctgactt ccagaaggct cattgcccac ccccgaagcg accttcggtc gatgccccct
      121 cgggcgtcgc cctctccccc tcacgggggc cgagacctgc ggctccaaac cgacctgcgt
      181 cggtttggac aggtcgagga atggccgcgt ctcgcggcca tgcggcctgc aaggcacacc
      241 agcggcccgg caaagccggt tccgcggtgg cccccgcact ggtcttgcgt acgcacgcgc
      301 tgcgggacta ccaacaacgc ggtggagctc tgacgtgagc ctgctgcaag tcaacaacct
      361 catcgtcgag ttcccgggcc gccgcggcac gctgcgggcc ttggacgaca tctcgttcga
      421 catcgcgccc ggcgagatcc tgggcgtggt gggcgagtcc ggcgcgggca agtcgctcac
      481 gggcgcttcc atcatcggcc tgctggagcc gcccgggcgc gtggtgtcgg ggcagatcct
      541 gctggaaggc cggcgcatcg acaacctgcc gcccgagcag atgcgccaca tccgcggccg
      601 caagatcggc gccatcttcc aggacccgct gacgtcgctg aacccgctgt acaccgtggg
      661 ccggcagctg accgagacca tccaggcgca tttgcccgtg aatatcggcg aagcgcgcaa
      721 gcgggccgtt ggattgctgg aagacaccgg catccccgcg gcggccgagc gcatcgacca
      781 ctttccgcac cagttctccg gtggcatgcg ccagcgggtg gtgatcgccc tggccctggc
      841 ggcggagccg aagctgatcg tggccgacga gcccaccacg gccctggacg tgtcgatcca
      901 ggcgcagatc atccagttgc tcaagcgcgt gtgcaaggac cgcggcgccg ccgtcatgct
      961 gatcacgcac gatatgggcg tgatcgccga aacctgcgat cgcgtcgccg tgatgtacgc
     1021 ggggcgcatc gccgagatcg gaccggtgca cgaggtcatc aaccagccgg ctcaccccta
     1081 tagcatgggg ctgatggcag ccattcccga tatcaccatc gagcgcgagc ggctgaacca
     1141 gatcgacggc gccatgccgc ggctggatgc gattccgccc ggctgcgcct acaacccgcg
     1201 ttgtccgcgc gccttcgacc gctgcatcgc cgagcgtccg gatctgctcg aggccggcgc
     1261 cacgcgcgcg gcgtgctggc tgcacgacgc gcatgcgggg gtgacatgaa ccagcaattt
     1321 ttccccctct ccctttggga gagggttagg gtgagggtat tgcggcggtc cttccctcac
     1381 ccctgccctc tcccagaggg agagggagga aatccgtcat gaatgccccg ctggtccagg
     1441 cccacgacct cgccaagact ttcgacgttt cagtgccctg gctcaaccgg gtgatcgagc
     1501 gccggccacg ccagctgctg aaggcggtgg acggcgtgag tttcgaaatc ggcaagggca
     1561 agacgctggc gctggtcggc gagtccggtt gcggcaagag cacggtggcg cgcctgctgg
     1621 tggggcttta tgagcccacg cgcggcggcc tgaccttcga cggccagaac gcccacgcgg
     1681 cgttcaaggc cccgcaaggc cgacagttgc gccgccgtat ccagatgatc ttccaggacc
     1741 cctacgccag cctgaacccg cgctggatcg tggaggacat cgtgggcgag ccgctgaagg
     1801 aacacggcat catcaccgac gctgcgcagc tcgaggttcg cgtgggcgag ctgctcgagt
     1861 cggtggggct gtcgccgttg gacatggcga agtatccgca ccagttttcc gggggccagc
     1921 gccagcgcat ctcgatcgcc cgcgccctgg cgaccgagcc cgagttcctg gtgtgcgacg
     1981 agccaacctc ggcactggac gtgagcgtgc aggcccaggt gctcaacatc atgaaggacc
     2041 tgcagcgcca gcgcggcctg acctatctct tcatctcgca caatctggcc gtggtgcggc
     2101 atgtgagcga ccaggtaggc gtgatgtacc tcggccggat agtggagctc gcggacaagc
     2161 gcgagctgtt cggcaatccc cgccatccct acacgcggat gctgctcgac gcgatcccga
     2221 agatgcacga caccggccgg cgacgcacgc cggtgcaggg cgaggtgccg aatccgctca
     2281 atccgccgac tggctgcgcc ttccatccgc gctgtccgca tgccaatgcg cggtgcaagg
     2341 ccgagcggcc ggcgctgctg gaaatacgag gcgtgaagat tgcgtgtcac gccgttgaag
     2401 aggggcggct ctagacctgc acgctcgccc agaggctgtc atgccggacc ccggatcgag
     2461 tccggggcag gcttgatccg gcatccatga cttcctggat tgcggatcga gcccgcaatg
     2521 acaagaaacc ggtcatgccg ggcttgaccc ctggttgtca tcccgggctt gacccgggat
     2581 ccatgcttcg ttcacgacgt ttcatccagc gcgtaacatg gattgcgggt caggcccgca
     2641 atgacagaac cgttacgcgt agcgcttgtt gcgcaggttg ttcttcatct cgcgcaacag
     2701 cggctgcagg cgcggcccga tgcgcagcgc cacggcggtg gccagcacat cgatgatcac
     2761 caggtgcaac aggcgcgaca ccatggggct gtagcggtcg tagccttccg ggtggtcggc
     2821 cgccaggtgg atctgtccgg ccgacgcgag cggcgagccc gtggccgtta tgacgatcgt
     2881 ggtcgcgccg ttcttgcgcg cgatgtcgca ggcatccatc aggtcgcgcg tgcggcccga
     2941 gttggaaatg acgaccacgc aatcgcccgg tccgagcagg gtggcgctca tcacctgcat
     3001 gtggccgtcg ctgtaggcga tggtgttcac gcccaggcgg aagaacttgt gctgggcgtc
     3061 ctgcgcgacg atccccgaat tgccgacccc gtagaactcg atgcgccggc cggtcttgca
     3121 ggtggaggcc agcgcctcca cggccttctc gaccgcgaaa gtcgaggcgt cgttgcggta
     3181 cttgaggaac gccgccaccg tgttgtcgat caccttcacc agcacgtcgc cggttttgtc
     3241 gtcggcgtcg acgctgcggt ggatgaaggg cacgccctcg ctgacgctgc cggcgagctt
     3301 gagcttgaaa tcggacagcc cgtcgtagcc gatgctgcgg caaaagcgca ccacggtggg
     3361 cttgctcacg tgcgcccggt cggccagctc gcttaccggc agattggcga aggcgcgtgg
     3421 atcgctcagc accagccggc cgacccgctg ttcggccggg gccagcgaag gcagcgacgc
     3481 cttgatgcgg tctaacacgg cgccgccccg ccgcccgaag gcgcgtgcgc ccccccgggg
     3541 ggcagcgatg gcccgtcggg ccaggcgcgg gggctcaaca ttcttcgctc cagcaatgcc
     3601 cgtcgcgcgc gatcatcgcg ctggcggcgc tggggcccca ggtgccggcc gcataaggcc
     3661 gcggtcccga agtctcgccg tcccagtatt ccatgatcgg ttcgacccag cgccaggctt
     3721 cttcctgctc gtcgctgcgc acgaacaggt tgagccttcc gtcgatcacg tcgagcagca
     3781 ggcgctcgta ggcaccgacg cgctcggagc cgaaacgctt gtcgaagtcc aggtccagct
     3841 gaacctgcgt cagcgactgc ccggcgcttc tcgaacgttg gcggttctcc ggtccctgcg
     3901 ccagcaggtg cagctccagc ccgtcgttgg gctgcagttt gatcaccagg cgattggccg
     3961 cgccgttggg cgagtggaag atcgcgtgcg gcgccggctg gaaattgacc acgatgcgcg
     4021 catcggccgc ggccaggcgt ttgccggtgc ggatgtaaaa gggcacgccg gcccagcgcc
     4081 agttggcgat ctcggcgcgc agcgccacga aggtttcggt cgtgctttgc gggtcgacgc
     4141 ccggctcgtc gagatagccc ggcaccgttt cgccggccac tcggccggcc atataccggc
     4201 cgcgcaccac atgctgggcc acggactgct gcgtccaggg cttgagcgag cgcagcacct
     4261 tgagtttctc gtcgcggatc gcgtcggcgt gggcgttgat cggcggctcc atggcgagcg
     4321 cgcaaagcag ctgcagcgcg tggttctgca ccatgtcgcg caacgccccg gtgctgtcgt
     4381 agaaggcgcc gcggttttcc gtgcccaggt cctcggcgag cgtgatctgg atgttggcga
     4441 tggtctcgcg ccgccacagc ggctcgaaca gggcgttgcc gaagcgcagc gcgaacaggt
     4501 tctgcaccga cggcttgccc aggtagtggt cgatgcggaa gatctgcttt tcgcccagca
     4561 cgcgtcgcac gcaggcgttg atcgcgcgat tcgaagccag gtcgtgcccg agcggtttct
     4621 ccagcacgag gcgggtgttg gcggtgttca ggccggcggc ggcaagctgc tcgcagacca
     4681 ccgtgaagag cgaaggcgca gtggccaggt acatcacgat ggtggcggcc tgccgctcgc
     4741 gcagcgtcgc cgccagccgc tggtagtccc cgggctgcga caggtccatg cgcaggaagc
     4801 gcagcagggc cgcgaagcgc tcgaattccc cggggctggg ccgcttcgcc agctcgacct
     4861 gatcgaagcg cgaccttatc agcgatcggt attgcccgtc gtccaggttg tcgcgcgcaa
     4921 cgccgatgat gcgggccccc tcgggcaggc tgccgtggcg gaacgcctgg aacagggcgg
     4981 gcatcagctt gcgccaggca aggtcgccgg tgccgccgaa caggacaaga tcgaagctca
     5041 tggtcggatc aaggaaacga tccggtactg taacttagtt tcaaggtgcg gtgggtgcgt
     5101 cgccgcctgg ccggctgtgc cattcgaccg ggcctccccc gttccggcgg gaggggcctt
     5161 tgccatgttt gagccgtaac caagttactt cgacgacatg cgggtgagcc acgccggcgc
     5221 cagggcccgc tcgataatgc ggccatgaac cccaccaatc catcgctgat cccctgcgcg
     5281 ctgaacgaag aggccatgtc caccgagaaa ctcgccgagg gaatccgcgc ctttgcagcc
     5341 gacgccgtga agctcgaaca gctgatgaag gcggcctgag ccatggccgc aagcatcaca
     5401 cggccgcgct gcgacgagac gaccgcctgg gcggcattgc aggaacactt cgcggccatt
     5461 gggcgcacgc tggacctgcg cgatgccttc cggtgcgacc ccggccgctt cgagaccttc
     5521 agccaggagg cgccccatgt gttcgccgac ctgtccaaga acctgatcga cacccgggcc
     5581 gaggcgctgc tgctggaact ggcgcgcgcg tgcgggctcg agggccatcg cgacgccatg
     5641 ttcgcgggtc atgcgatcaa cagcaccgag cagcgtgccg tcaagcactg gctgctgcgc
     5701 gcgccgggcg gcgccggcac cgacccggac gcggcgcagg tgcacgccac gctggatgcc
     5761 atgctggcct acgcggagca ggtgcgcgcc gacgaagcga tcaccgacgt ggtgaacatc
     5821 ggcatcggcg gatccgacct cgggccgcag atggccgtgg cggcgctgga cgccttcgtc
     5881 ctgccgggca agcggctgca tttcgtgtcc aacatcgacg gccacgaact cgccgccgtg
     5941 ctgcgccggg tgcgaccgga aagcacgctc ttcctggtgg cctccaagac cttcaccacg
     6001 atcgagacca tgaccaacgc gcgctcggcg cgcacctggt tcgaagccga gggcggccgc
     6061 gaccccgggt cggggcccgg ggcaggcgtc gcgcgccact tcgccgcgct gaccaccaac
     6121 gtggcggcgg cgcgcgagtt cggcatcgcc accaccttcg ggttctggga ctgggtgggc
     6181 gggcgttact cgatgtggtc ggccatcggc ctgcccatcg ccatcgcggt gggcgcggcg
     6241 ggttttcgcg aactgctggc cggcgcgcac gagatggacc ggcacttcgc taccgagccg
     6301 ctggcgcgca acctgccggt ccggctgggg ctgctcgacg tctggaaccg caacttccac
     6361 ggcttcgcga gccgcagcat cgcgccctat cacagcgcgc tgcgccgctt gccggcctac
     6421 ctgcagcagc tggaaatgga gagcaacggc aagcgcgtcg atgctggcgg ccggcccctg
     6481 tcgcaggctt cggcaccggt gttgtggggc gagcccggca ccaacggcca gcatgcctat
     6541 ttccagatgc tgcaccaggg cacggacgtg attccagtcg agttcatcgc ggtgaaaacg
     6601 ccggcgcatg gcctggccgg ccatcacgaa ttgctgctgg ccaacgcctt ggcccaggcc
     6661 caggcgctga tgcagggcaa gcccgacgcc ggcggccacc gcgatttccc gggcaatcgc
     6721 cccagcactt tcttcctgct ggagcggcta acgcccgcca gcctgggtgc gttgctggcc
     6781 ttgcatgaac accgggtctt cgtcagcggt tcgatctggg gcatcaacag cttcgaccag
     6841 tggggcgtgg agctgggcaa ggtgctggcg ctcgacatcg cgccgcggct ggccagcggc
     6901 gacgcgtccg ggctcgatgc ttccacggcc ggcttgctgc accggctggg gacgccgccc
     6961 gcttgagggt ggcgctgcgc ccgccgaacg catatcatgg cgcgggcatt tgcccgggac
     7021 acgcgaggag aatcgttcat gaacttcagc caagccgttg ccacctgcct gcgcaagtac
     7081 gtcgactttt ccggacgcgc agcccggccg gaattctggt ggtttttcct gttccaggtc
     7141 atcgtgatgg ccgtgaccgg gatgatgagc gacatcctga acgggatcgc ggcactggcg
     7201 ctgctgctgc ccggcctttc ggttggcgcc cgccgtctgc atgacatcgg caagagcgcc
     7261 tggttcctgc tgctgggcct gattcccatc ctgggcttcc tggtcctgct gtactggttc
     7321 gtgcagccca gtgcccccgc caacgcctac ggcccggccg aatccggttc cgatgctccg
     7381 acggtcatgc ccggccagca ataaaaaagc cgatcgaaag agcggccttc gttcccggtg
     7441 cgcggaataa catgcagcgc accttccaat gaaaaaggcc gggtttcccg gcctttttcg
     7501 tctggctagc cactgcacaa aacgcgtagc gtttttgcag tgaacagggc ttacatgtcc
     7561 atgccgccca tgccgcccat gccacccata ccgccgccca tgccaccacc ggcgccggat
     7621 tcatccttcg gcgcttccgc caccatggct tcggtggtca gcatcagcga cgcgaccgac
     7681 gcgg
//
LOCUS       DASWIY010000219         5168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2721475, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000219 DASWIY010000000
VERSION     DASWIY010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721475"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VGA59_08010"
     CDS             complement(<1..378)
                     /locus_tag="VGA59_08010"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9719661.1"
                     /translation="MHEQAGKPSAAGAEGTALRILSQAVAFLSSAAMALSAAGLLLSL
                     GLIGWAVVMRYVFNAAPIWVDEVVGFLLVAIVLLAAAQTFRRGEHIGVDLLVGRLAPT
                     GKRWALGWAAVGTACVALVLIVNG"
     gene            complement(393..1400)
                     /gene="dctP"
                     /locus_tag="VGA59_08015"
     CDS             complement(393..1400)
                     /gene="dctP"
                     /locus_tag="VGA59_08015"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9719662.1"
                     /translation="MTRLNTAIGRRTLIAAALGAGFAGQALAADYTMRISHQFPPTHH
                     TAVNLGQFEKEVEAATKGRVDVQIFGAAQLFKPNQNHPAVASGKIEAATILSFQWGGT
                     IPEMSVTIIPYLMTSVAKQKAFMNSEASKLLDAKMEAKGVKNIAWIVDTNDGIFTSGK
                     RLLATPADFAGLKIRGLNKLFDAGLAAMGASPSAMPGSEVYQALQTGLLDAGFTGVKA
                     ANSRKFYEIQKYGVASNIILAFDNLVVNPAWWNGLPADVRGAIQQAANKAVRRSIRTT
                     DGVPPEDVKVLNEKGMKTIALTKAQEKVMADAMQPAVKKEFLSATAPDGVKLLESIDR
                     L"
     gene            complement(1430..2644)
                     /locus_tag="VGA59_08020"
     CDS             complement(1430..2644)
                     /locus_tag="VGA59_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9719663.1"
                     /translation="MSDTPQPTPRTPQPLAGLRVVEFTHMVMGPTCGMVLADMGAEVI
                     KVEPIDGDRTRRLLGAGAGFFPMFNRNKKSIAIDLHKSEGAEVARRLAASADVVAENF
                     KPGTMVKYGLDYATLSQVNPRLIYVAHKGFLPGPYEHRTALDEVVQMMGGLAYMTGRP
                     GDPLRAGTSINDIMGGLFGAIGALGALIQRGISGRGMEVQSALYENNVFLMGQHMLQF
                     AMTGKHPAPLPARDNPWAVYDVFTVKDGEQIFLAAVSDAQWIAFCKVLGLDDLGSDAT
                     LATNNDRVKMRPQLLPMLRERLAGRSAAELSTLMEQAGLPFAPIRRPEDLFDDEHLLA
                     TGGLADVRLPDGPNAGRTVKTTLFPITMDGDRLGVRLDPPRLGENTTGLLQGLGYSLS
                     DIQALRDSLAVA"
     gene            complement(2641..3585)
                     /locus_tag="VGA59_08025"
     CDS             complement(2641..3585)
                     /locus_tag="VGA59_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX9719664.1"
                     /translation="MNIPNEPIVVREVGLRDGLQSIARVLPTENKLEWIRGAYEAGQR
                     EIEVGSFVPARLLPQLADTAELVAFAKTLPGLVTSVLVPNLKGAERAIEAQADLMLLP
                     LSASHAHSLANLRKAPDEVVADIARIRAARDASGSKCLIEVGMSTAFGCTIQGRVEPE
                     EVLRLLRSVLDAGADRVGLADTVGYADPRMVHDLFERSFAVAGERLACGHFHDTRGLG
                     MANVMAALDTGVTRFDACLAGIGGCPHAPGASGNVATEDLAYLFASMGLHTGLDIDRL
                     LALRAKVAQWLQGETLHGTLWRAGLPKTMRRDAVEPAQ"
     gene            3723..4619
                     /locus_tag="VGA59_08030"
     CDS             3723..4619
                     /locus_tag="VGA59_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9719665.1"
                     /translation="MRDLDLTSQRLFVAACETRNIARAAAQASLVSSAVSKRLAQLEA
                     QLGTPLLVRRRHGVEPTAAGESLLEHARGMLVSAQRMERDMAAFAAGVRGQVRILASA
                     SAMAESLAEEVAAFLQQPGHRDIRVDIEERISPQVIQGIRDGVASVGICWDAADLRGL
                     QSRPYRQDHLAVVVHAGHPLADRIDMPFAQALEWEHVGMPVDSAVQVRLVRAAAEQGR
                     SLTFRVIVSSFEAALRVVRARLAISVMPREIAEPYAATHGLRVVPLTDPWAQRRFTLC
                     YRDELLLTPAARLLVDHLGSMA"
     gene            complement(4754..4846)
                     /locus_tag="VGA59_08035"
     tRNA            complement(4754..4846)
                     /locus_tag="VGA59_08035"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4810..4812),aa:Ser,seq:gct)
     gene            complement(5035..>5168)
                     /locus_tag="VGA59_08040"
     CDS             complement(5035..>5168)
                     /locus_tag="VGA59_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX9719666.1"
                     /translation="EEGINIQMISTSEIKTSVVIDEKYMELAVRALHRAFDLDQPAA"
BASE COUNT          818 a         1725 c         1732 g          893 t
ORIGIN      
        1 gccattgacg atcagcacca gggccacgca ggcggtcccg acggcggccc agcccagtgc
       61 ccagcgcttg ccggtgggag ccaaccggcc gaccagcagg tccacgccga tgtgttcacc
      121 ccggcggaaa gtctgggccg cggccagcag gacgatggca accagcagga agccgaccac
      181 ctcatcgacc cagatcggcg ccgcattgaa cacgtagcgc atgaccaccg cccaaccgat
      241 cagccccagg gagagcagca gcccggcagc cgagagtgcc atcgcagcgc tggagagaaa
      301 agccaccgcc tggctcagga tgcgcaaggc ggtgccctcg gcccccgcag ccgagggctt
      361 gcctgcctgc tcgtgcatat ccgcgcgccg cttcagagtc tgtcgatcga ttccagcagc
      421 ttcacgccgt ccggcgcggt ggcgctgaga aactccttct tcaccgccgg ttgcatcgcg
      481 tcggccatga ctttctcctg cgccttggtc agtgcgatgg tcttcatgcc cttttcattg
      541 agcaccttaa cgtcttcggg tggcacgccg tcggtggtcc ggatggatcg ccgcacggcc
      601 ttgttggccg cttgctggat ggcgccacgc acatcggcgg gcaggccgtt ccaccaggcc
      661 gggttcacca ccaggttgtc gaacgccagg atgatgttgg aggccactcc gtacttctgg
      721 atctcgtaga acttgcggct gttggcggcc ttcacgccgg tgaatccggc atcgagcaag
      781 cctgtctgca gcgcctggta gacctctgag ccgggcatgg ccgagggaga cgcgcccatg
      841 gctgccaggc cggcgtcgaa gagcttgttc aaaccgcgga ttttcagccc ggcgaaatcg
      901 gccggcgtgg ccaacaacct cttgccggag gtgaagatac cgtcgttggt atcgacgatc
      961 caggcgatgt tcttcacgcc cttggcttcc atcttggcat ccagcagctt cgaggcttcc
     1021 gagttcatga aggccttctg tttggccacc gaggtcatca ggtagggaat gatggtgacg
     1081 ctcatctccg ggatggtgcc gccccactga aagctcagga tggtcgccgc ctcgatcttg
     1141 ccgctggcca cggcgggatg gttctggttg ggcttgaaca gctgcgcggc gccgaagatc
     1201 tgcacgtcca cgcgcccctt cgtggctgcc tccacctctt tctcgaactg ccccaggttc
     1261 accgccgtgt ggtgcgtggg cggaaactgg tggctgatgc gcatcgtgta atcggcggcc
     1321 agggcctggc cggcaaagcc ggcgcccagc gccgccgcga tgagggtgcg gcggccgatg
     1381 gcggtattca agcgggtcat gtcttgtctc cttcgttgtc atcaaaaaat caggccacgg
     1441 cgagcgagtc gcgcaaggcc tggatgtcgg acagactgta ccccagccct tgcagcagcc
     1501 cagtcgtgtt ttcacccagt cgcggcgggt ccagccgcac tcccagccgg tcaccgtcca
     1561 tggtgatggg gaacagcgtg gtcttcacgg tccggccggc gttggggcca tcgggcagtc
     1621 gcacgtcggc caggccaccg gtcgccagca ggtgttcgtc gtcgaacagg tcttcgggcc
     1681 tgcggatggg cgcaaagggc aggccggcct gttccatcag ggtcgacaac tcggccgcgc
     1741 tgcgcccagc cagccgctcg cgcagcatgg gcaacagttg cgggcgcatc ttcacgcggt
     1801 cgttgttggt cgccagcgtg gcgtcgctgc ccagatcatc caggccgagc accttgcaga
     1861 acgcgatcca ctgcgcgtcg ctcacggcgg caagaaagat ctgctcgccg tccttgacag
     1921 tgaacacgtc gtacaccgcc cacgggttgt cgcgcgccgg caggggcgcg gggtgtttgc
     1981 cggtcatggc gaactgcagc atgtgctggc ccatcaggaa cacgttgttc tcgtagagcg
     2041 cgctctgcac ttccatgccg cggccggaga tgccgcgctg gatcagcgcg cccagcgcgc
     2101 caatcgcgcc gaacaggccg cccatgatgt cgttgatgct ggtgcccgcg cgcagcgggt
     2161 cgccagggcg ccccgtcatg taggccaggc cacccatcat ctggaccacc tcgtccaggg
     2221 cggtgcggtg ttcgtagggg ccgggcagga agcccttgtg ggcgacatag atcaggcgcg
     2281 gattgacttg cgagagagtg gcgtagtcca ggccgtactt gaccatggtg cctggcttga
     2341 agttctcggc caccacgtcg gcgctggccg cgagccggcg cgccacctcg gcgccctcac
     2401 tcttgtgcag gtcgatggcg atgcttttct tgttgcggtt gaacatcggg aagaagcccg
     2461 cgcccgcccc cagcagccgg cgcgtgcggt cgccgtcgat gggctcgacc ttgatcacct
     2521 cggcgcccat gtcggccagc accatgccgc aggtggggcc catcaccatg tgggtgaatt
     2581 caaccacccg caagccggcc agcggttgcg gcgtccgggg ggtcggttgc ggcgtgtcgc
     2641 tcattgggca ggctccacgg catcgcgcct catcgtcttg ggcaggccgg cgcgccagag
     2701 cgtgccgtgc agggtctcgc cctgcagcca ttgcgccacc ttcgcgcgca gggccagcag
     2761 gcgatcgatg tccagaccgg tgtgcaggcc catgctggca aagaggtaag ccagatcttc
     2821 ggtggccacg ttgccgctgg cgccgggcgc atgcgggcag ccgccgatgc cggccaggca
     2881 ggcgtcgaag cgcgtcacac cggtgtccag cgcggccatc acgttggcca tgcccagccc
     2941 tcgcgtgtcg tgaaagtggc cgcaggccag ccgctcgccc gcgacggcaa agctgcgctc
     3001 gaacaggtca tgcaccatgc gcggatcggc ataacccacg gtgtctgcaa ggcccacgcg
     3061 gtcggcgccg gcgtccagca ccgagcgcag caggcgcagc acctcctcgg gctcgacccg
     3121 gccctggatc gtgcagccga atgccgtgct catgccgact tcgatcaggc atttggagcc
     3181 agacgcgtcg cgcgcggcgc ggatgcgggc gatgtcggcc actacctcgt cgggcgcctt
     3241 gcgcaagttg gccaggctgt gcgcgtggct ggccgagagc ggcagcagca tcaggtcggc
     3301 ttgcgcctcg atggcgcgct cggcaccctt gaggttgggc accagcacgg acgtgacaag
     3361 gccgggcagg gtcttggcga aagccacgag ctcggccgtg tcggccagtt gcggcagcag
     3421 gcgcgccggc acgaaggagc cgacctcgat ctcgcgctgg cccgcctcgt aggcgccgcg
     3481 gatccattca agcttgtttt cggtgggcag cacgcgggcg atgctctgca ggccgtcgcg
     3541 caggccgact tcgcgcacca cgatgggctc attggggatg ttcaagcagt ctcctgatgg
     3601 ggtcacggcc gggttgagcc tcggtctagg acgaagtgta gagttcacga aaatgcggta
     3661 aagcggtatt ttggaagcct tgacgttcca tatcgggaat tcataagcaa ggagactgac
     3721 tgatgcgcga cctcgacctc accagccagc ggctgttcgt cgccgcctgc gaaacccgca
     3781 acattgcccg ggccgccgcg caggccagcc tggtgagcag cgcggtgagc aaacggctcg
     3841 cgcagctcga agcgcaactt ggcacgccgc tgctcgtgcg ccggcgccac ggggtcgagc
     3901 ccacggccgc cggcgaaagt ctgctggagc acgcgcgcgg catgctggtc agcgcacagc
     3961 gcatggagcg cgacatggct gccttcgccg ccggcgtgcg cgggcaggtg cgcatcctgg
     4021 catcggcctc ggcgatggcc gaatcgctgg ccgaggaagt ggcggctttc ctgcaacagc
     4081 ctggccatcg cgacatccgc gtggacatcg aggaacgcat cagcccccag gtgatccagg
     4141 gcatccgcga cggcgtcgcc tcggtgggca tctgctggga cgcggccgac ttgcgcggcc
     4201 tgcagtcgcg gccctatcgg caggaccacc tggcggtggt cgtgcatgcc ggtcacccat
     4261 tggccgatcg catcgacatg ccgttcgcgc aggcactgga gtgggaacac gtgggcatgc
     4321 cggtcgacag cgcggtgcag gtgaggctgg tacgcgcggc agcggagcaa ggccgcagcc
     4381 tgacttttcg cgtgatcgtc tcgagcttcg aggccgctct gcgcgtggtg cgcgcgcggc
     4441 tggccatcag cgtgatgccg cgcgaaatcg ccgagcccta tgcggcgacc catggcctgc
     4501 gcgtggtccc gctcaccgac ccctgggcgc agcgccgctt cacgttgtgc tatcgggacg
     4561 agctgctact gacacccgcc gctcgcctgc tggtcgatca cctgggttcg atggcgtaac
     4621 tggcccgctc agccctgcaa cgtgctgcgt gccctttatt gcgcgcgatc atcatccatg
     4681 gatgcatgcg accaacttct gcgtgctttt gtcggagcga aaaacaaaac gccccgatgg
     4741 ggcgtttgcg cattggcgga aacggaggga ttcgaaccct cgatgaggct caacacccca
     4801 tactccctta gcaggggagc accttcggcc actcggtcac gtttcctgta gccgggaatt
     4861 atgccccaac ccggtccgtc cgaggtcgcg gcaggagaaa accagctcaa catctgcgcg
     4921 tgggtcccgg ccttcgccgg gacggcagca tgcgccgccg ctttgcggcc gctcatcaat
     4981 gcatgggtcc cggcctgcgc cgggacggca gcgtacgcgc cgctttgcgg ccgctcacgc
     5041 tgccggttgg tccaggtcga aagcccggtg cagcgcgcgc acggccagct ccatgtactt
     5101 ctcgtcgatg accacggaag tcttgatctc gctggtcgag atcatctgga tattgatccc
     5161 ctcctcgc
//
LOCUS       DASWIY010000220        15976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2368919, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000220 DASWIY010000000
VERSION     DASWIY010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 15976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15976
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2368919"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VGA59_08045"
     CDS             <1..303
                     /locus_tag="VGA59_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX9719667.1"
                     /translation="ALRADVLLVPSAFTYVTGQAHWELLLRARAIENLAYVIAPAQGG
                     VHENGRRTWGHSMVVDPWGGVLAERAEGPGIVIAELDAGRQARLRLQLPALEHRVI"
     gene            300..2372
                     /locus_tag="VGA59_08050"
     CDS             300..2372
                     /locus_tag="VGA59_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9719668.1"
                     /translation="MNAAQASPHASAPFGPRRPPAYRRWSLWALLIALVAALLVTLVW
                     LAGRYEASQVQSKLERDTADALSDIRSALTRNVQTLQALQAGQPSRASWSSEAAGVLR
                     EHREWVRIEWRDSALRTFAAVDTPFRAPVFARLGRANAQADVALACANGRRVSGPAYS
                     SSYFVPQLDGLGLEVMELCMPQVTAGELTGYVVATYSLSEILSGMIGPQLMRSQEISF
                     TEADGTRLAMHGGIRRGARVFTSQQLLDLPGNTMVLRMDSWRGAPDLFPNVLTALVTA
                     MSIALVSVLVLLGKDMRRRLRAEQDLADALAFRKAMEDSLVTGLRARDLQGRITYVNP
                     AFCQLVGFSAEELLGNTGPAPYWPPELAGQYQKRQEIRLTGEHAPPREGYESVFMRKD
                     GSRFPVLIIEAPLINHHGIQTGWMSAFLDMSEQRRIEEISRASQERLQATARLATVGE
                     MASLLSHELNQPLAAISSYATGTINLLKTQMASQTTSDGDGGPTPDIEVAMRRIAEQA
                     ERAGKVIKSVHDFVRRRDKEREPVAPQALLDAIMPLVSLQARKLGVRVETRLMRGLPD
                     VFCDRTMVEQVLLNLARNAMQAMDQVHVTDPSLLIEVRRATAVGGETTPGKSWLEFLV
                     TDVGPGIPEPVKQQLFTPFFTTKAEGMGLGLSLCRTVVEQHGGFLGFEPNRPQGTIFR
                     FTLPVDRS"
     gene            2408..3031
                     /locus_tag="VGA59_08055"
     CDS             2408..3031
                     /locus_tag="VGA59_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719669.1"
                     /translation="MQPAANATVFIVDDDASVREALAWLLRSRHLLSEGYAGGAEFEA
                     MLQGYTPAQPCCLLLDVRMPGMSGLALFDKLAERGLVEVMPVIFLTGHADVPTAVDLV
                     KRGAFDFCEKPFSDNALVDRIEHAIGRSQQVLAARREKLLLQGRLAELTERERDVMRL
                     VVEGLPNKLIADQLDISVRTVEVHRARIFDKMEVKSAVELVNVLRHV"
     gene            3157..4299
                     /locus_tag="VGA59_08060"
     CDS             3157..4299
                     /locus_tag="VGA59_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEX9719670.1"
                     /translation="MNSMNRFLWTAPIVGMLVGCASVPLPPTDEGPARPAAEPIGPGD
                     TGPLPPPLLQGKSRWQPVRWAELPGFGEDALHEAWNAWLKSCERPSPAFARVCPEVRR
                     LSIAAADEQRAWLMQRLQPYRVEPLQGPAEGLLTGYYEPMLEASRRPTATHQVPLFRP
                     PATLGSRRPWYTRQEIDTLAEARAALRGREIAYVADPLDALVLQIQGSGRVRITEPDR
                     SVRTVRLAYAASNDQPYRSVGSWLLQQGAIRDASWPGIKAWARDNPQRVHEMLWSNPR
                     TVFFREEPLGEFDAAFGPRGAQGVPLTPGRSIAVDKDSIPYGTPVWLVSRGPVTSLQR
                     LVLAQDTGSAIVGAVRADYFTGWGPEAGELAGRLKQPLRLWVLWPR"
     gene            4379..4738
                     /locus_tag="VGA59_08065"
     CDS             4379..4738
                     /locus_tag="VGA59_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719671.1"
                     /translation="MAKLPVAGVLTAAVLWGCTATAPVPSASPAAPPGPAASTARYRC
                     EQNIEFTVRFAEDSAMVDAGPRGNEVLLRDAGGTTPLQTVYTNARLRAEFGLGVSGRE
                     AILRYPLVPLVARCVRQ"
     gene            complement(4746..5231)
                     /locus_tag="VGA59_08070"
     CDS             complement(4746..5231)
                     /locus_tag="VGA59_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9719672.1"
                     /translation="METLDKFDAAILRELQADGRLTNAELAQRVGLSAAPCWRRVRAL
                     EQAGYIKGYHAEIDRHKIGLGVLAFVRLDANRNTGELTREMEEAIRKIPEIVSCHYIS
                     GAGTFELQVVSRDLDTFSQFARKVLINLPNVKDLHTSFSLGEVKASSSLPLSHLSARP
                     L"
     gene            5416..8979
                     /locus_tag="VGA59_08075"
     CDS             5416..8979
                     /locus_tag="VGA59_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX9719673.1"
                     /translation="MNAPLPEHIRKALETVTLDDKYSLDYGRAFMSGVQALVKLPMLQ
                     RLRDAQAGKNTAGFISGYRGSPLGGYDQALWKAGKYLKAQNIVFQPGVNEELAATAIW
                     GTQQLGFAPAGTRKFDGVFGIWYGKGPGVDRTSDVFKHANLAGTTPWGGVIAVAGDDH
                     VAKSSTAAHQSDHIFKACGLPVFFPATVQEILDLGIHAFAMSRFSGVWAGMKTIQEIV
                     ESSATAMIDPERVQVRIPTDFQMPPGGVHIRWPDTALEQEARLFDYKWYAALAYIRAN
                     RLNCNVIESADDRFGLIASGKAYNDTRQALLDLGLDDGACRQLGIRLHKVGVVWPLEA
                     QGTREFAQGLREILVVEEKRQVIEYQVKEELYNWRPDVRPNVIGKFNEVGGDQSGGEW
                     SQPNPMANTLLRANADLSPALIARAIAHRLKKLGVDGDMGARIDAQLAVLEAKERSMQ
                     VLEVKADRAPWFCSGCPHNTSTVVPEGSRAMAGIGCHFMATWMDRATIGFTQMGGEGV
                     PWVGQQPFSTEQHVFANLGDGTYFHSGLLAIRQSIAAGVNITYKILYNDAVAMTGGQQ
                     VGERPEGHSVNQIAHSLMAEGVTREVVVTDEPQKYTVAKLPPGVEVHHRDELDRIQRE
                     FRQIKGTTAIIYDQTCATEKRRRRKRGAMVDPAVRVVINDLVCEGCGDCSVKSNCLSV
                     EPLETEFGRKRTINQSTCNKDMSCLKGFCPSFVTVEGGNMKVKTRAQAPLSLGAFGEL
                     PEPALPALDGAYGIVVAGVGGTGVITIGQLLGMAGHIEGKGIVTQDAAGLAQKGGATW
                     SHVLIGERQDDIRTTRVSMAGADLVIGCDPIVTAGKETVLRMRLGRTHVALNAHSAPT
                     AAFVKNADWANPGDACAAEIVKAVGPEGVAAFNADAAATKLMGDSIYTNPMMLGYAWQ
                     KGWIPLGRESLLRAIELNGVAVENNKAAFTWGRRAAHDRAAVESLLAPAQVIEFKKRE
                     TLEGMVERRAQFLTAYQNTAYADSYRNFVDKVRLAEAPLGKTALTEAVARYLFKLMAY
                     KDEYEVARLHAETGFQEKIAAMFEGDYKVNYHLAPPLVARRNEKGELQKKKYGPAMLT
                     AFRVLAKLKGLRGTPFDIFGTTSERRAERALPAEYRQSIEQVIASLNAANHSQALEIA
                     RIPDQIKGFGHVKERNLVAARGHWQQLMAQWHDAQGERRAA"
     gene            9140..9457
                     /gene="yajC"
                     /locus_tag="VGA59_08080"
     CDS             9140..9457
                     /gene="yajC"
                     /locus_tag="VGA59_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX9719674.1"
                     /translation="MFISSAFAQTAGGDIQSSLMSMLPLILMFVVLYFVMIRPQMKRT
                     KEHRMMVEALAKGDEVATAGGMLGKVTKLGEVYIGVEIANGIEVQVQRSAVVQVLPKG
                     TIK"
     gene            9505..11388
                     /gene="secD"
                     /locus_tag="VGA59_08085"
     CDS             9505..11388
                     /gene="secD"
                     /locus_tag="VGA59_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX9719675.1"
                     /translation="MNRYPVWKYAIIVIALLMGAIYTLPNFFGEAPAVQVSPGKATVK
                     VDSTTRARVEQALKAAGIQPEAITLDANSLKVRLDSGDTQLRAKDAIQKALVPDPSDP
                     PYVVALNLLSASPKWLSALRAAPMYLGLDLRGGVHFMLQVDMQAALTKKAESLAGDLR
                     TILREKNIRHAGISRNGRAIEVRFRDTQTMEAAKAVIVDQFTDLQTAEVPEGTEYKLT
                     ATIRPEAGRRVQEQALKQNMVTLHNRINELGVAEPVIQQQGLDRIVVQLPGVQDTAKA
                     KDILGRTATLEVRMVDESTEARAAELGTGPVPFGDERYLERSGQAVVVRKQVILTGDN
                     LTDAQAGFDNQTQEPAVHLTLDAKGARLFKDVTRENVGKRMAILLFEKGKGEVVTAPV
                     IRSEIGGGRVQISGRMTTTEANDVALLLRAGSLAAPMEIIEELTIGPSLGAENIEKGF
                     QSVAWGFAVICAFMAVYYMLFGVFSAVALAVNLLLLVGVLSMLQATLTLPGMAAMALA
                     LGMAIDSNVLINERVREELRGGASPQAAINTGYERAWGTIFDSNITTMIAGIALLAFG
                     SGPVRGFAVVHVIGILTSMFSAVFFSRGLVNFWYGRKKKLKTVSIGTVWRPDGDGAIT
                     KAE"
     gene            11412..12365
                     /gene="secF"
                     /locus_tag="VGA59_08090"
     CDS             11412..12365
                     /gene="secF"
                     /locus_tag="VGA59_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX9719676.1"
                     /translation="MEFFRIRRDIPFMQNALIFNVISFLTFAAAVFFLFNRGLHLSVE
                     FTGGTVMEVSYSQPADVEKVRRTVSGLGFAEVQVQNFGTSRDVIIRLPAQKGVSSAQQ
                     SEKVLAALKAGDPGVSLRRTEFVGPQVGEELATDGLKALAMVIAGIMIYLAMRFEWKF
                     AVAAIIANLHDVIIILGFFAFFQWEFSLAVLAAVLAVLGYSVNESVVIFDRIRENFRR
                     YRKMTTVQIIDNAITATISRTIITHGSTQVMVLSMLLFGGPTLHYFALALTIGILFGI
                     YSSVFVAAAIAMWLGIKREDLVKSAARKDEDPNDPNAGATV"
     gene            12371..14893
                     /locus_tag="VGA59_08095"
     CDS             12371..14893
                     /locus_tag="VGA59_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 domain-containing protein"
                     /protein_id="HEX9719677.1"
                     /translation="MSVSQLPQGSGARIARQAREQFVARAEALLPPLVQAIRSRLAEL
                     AGSGGSSRQMQERRDALLDFDRKGAAWAQATARVWRQALDPPAAPTHVRLESAGLELI
                     GDEVVERKILSSRLALAIQEKATWELNDLRLRMQYLEGGDELAATDVLRPEALSQLLV
                     EQWDGAELSHEAWALVHEVIQRHVLEHVPDAYRSVNEFLVAQGVMRDIDLSSRVRRGA
                     TAAPGRKSDPAGPGAHEAAGGDADGGFGGSWASRDGAAAAERSAPGGGYGRRAEAGSQ
                     GGGRGQGGGRGQGGGQGGGQVGYSGPDGTLQDGGRSQAAPDSRPAGARAGGVADETRL
                     MTSSPPLARARMRATGVLGQLKRLLTERVAGFDGNPPTRPSPALAEALSQQSMPGQAT
                     RAQGAGTVQEDFVYDAAAVQEVAIDLRQRTSELKKKATTSTEKATIEIVALMFQSILA
                     EERIPPAVRVWFARLQMPVLRVAIAEPEFFGSLEHPARQLIDRMGSCVMGFDAAAIGG
                     TALEIEIKRVVQVIEQYPETGRRVFQLVYDEFQKFLSKFLTETAPTQRLVSVAQQVEQ
                     KETLAIQYTIELRSMLADMPVRDEIREFLFKVWAEVLALSAVKNGPQHEDTLALKSSA
                     SELVWAASAKPNRSERARVIQDLPRLLQRLRQGMTLVGIQGKAQEAHIKAIGDTLADA
                     FLSKTEAIPHARIETMAKRLAHLEDFVTDDPATDLPLDAESIELMLGIDASTIEVVAD
                     GGSRPSAAMLAWAGELQVGNWFSLDHNGTILQVQYAWRSDRKQLHLFAARGGRSYLIQ
                     ARRLAAYLQAGLLLPAEEEALTVRATRDALAKLDANPERLLQ"
     gene            complement(14939..15427)
                     /locus_tag="VGA59_08100"
     CDS             complement(14939..15427)
                     /locus_tag="VGA59_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEX9719678.1"
                     /translation="MFEVLVFVYEHYWRGEACPELQQLGRKLSAHGFEPDEIRDALAW
                     LDGLNLAAQSTRLGGTASPAPEAAVLQSASSLRVYSVAEQDHLGAPCLGFVSFLESSG
                     VLAAPMREIVIDRAMAAPGDPVSIDDLKIIILMVYWSFGEEPDALVLDELCDPAQGRL
                     PH"
     gene            complement(15491..>15976)
                     /locus_tag="VGA59_08105"
     CDS             complement(15491..>15976)
                     /locus_tag="VGA59_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX9719679.1"
                     /translation="LSANFPKRNRIIAALSQGTLVVEAALRSGSLITARLAAEQGKDV
                     FAIPGSIHSPQSRGCHALLKQGAKLVETAQDVLEEIALPQARPAQRAANAANAPVHPG
                     GSRESGLLASLGFDPVSLDALVARTGVAPAQLQAQLLELELQGEVARLPGGLFQRVGA
                     G"
BASE COUNT         2767 a         5202 c         5496 g         2511 t
ORIGIN      
        1 gcgttgcggg ccgatgtgct gctggtgccc agcgccttca cgtatgtgac gggccaggcg
       61 cattgggaat tgctgctgcg cgcgcgtgcg atcgagaacc tggcttatgt gatcgcgccg
      121 gcgcaaggcg gcgtgcacga gaacggccgg cgcacctggg gccacagcat ggtggtggac
      181 ccctggggcg gcgtgcttgc cgaacgcgcc gaagggcccg gcatcgtgat cgcggagctg
      241 gacgccggcc gccaggcgcg gctgcgcctg caattgccgg cgctggaaca ccgcgtgata
      301 tgaacgctgc gcaggcgtcc ccgcacgctt ccgccccatt cgggccgcgc cgccccccgg
      361 cttaccggcg ctggtcgctg tgggcgcttc tgatcgcgct ggtcgcggcc ctgctggtga
      421 cgctggtgtg gctggccggc cgctacgagg ccagccaggt ccagagcaag ctggagcgcg
      481 acacggccga tgcgctcagc gatatccgct cggccctcac ccgcaacgtg cagacgctgc
      541 aggcgctgca ggcgggacag ccctctcgcg ccagctggag cagcgaggcg gccggggtgc
      601 tgcgcgagca ccgcgaatgg gtccgcatcg aatggcgcga cagcgcgctg cgcaccttcg
      661 ccgccgtgga cacgccgttt cgcgcgccgg tattcgcgcg cctggggcgc gccaatgccc
      721 aggccgacgt tgcgctggcc tgcgccaacg gccgccgcgt gagcgggccg gcctattcga
      781 gcagctactt cgtgccgcag ctcgacgggc tgggcctcga ggtcatggag ctgtgtatgc
      841 cccaggtcac ggccggcgaa ctcaccggct acgtcgtcgc cacctactcg ctcagtgaaa
      901 ttctgtccgg catgatcgga ccccagctca tgcgcagcca ggagatttcg ttcaccgagg
      961 cggacggcac gcgattggcg atgcacggcg gcatccggcg cggggcgcgg gtcttcactt
     1021 cccagcaact gctggacctt cccggcaaca ccatggtgct gcgcatggac agctggcgcg
     1081 gcgcgcccga cctgttcccc aatgtgctca cggcgctggt caccgcgatg tcgatcgcgc
     1141 tggtgtcggt gctggtgctg ctgggcaagg acatgcgcag gcgcctgcgc gccgagcagg
     1201 acctggcgga tgcgctggcg ttccgcaagg cgatggaaga ctccctggtg acgggcctgc
     1261 gggcgcgcga cctgcagggg cgcatcacct atgtgaaccc ggccttctgc cagctggtgg
     1321 gtttttcggc ggaggaactg ctgggcaata cggggcccgc gccgtattgg ccgcccgagc
     1381 tggccggcca gtaccagaag cgccaggaga tccgcctgac cggcgagcat gcgccgccgc
     1441 gcgaagggta cgagtcggtt ttcatgcgca aggacggttc gcgcttcccg gtgctgatca
     1501 tcgaggcgcc gctgatcaac catcacggca tccagaccgg ctggatgagc gccttcctgg
     1561 acatgagcga gcagcggcgc atcgaggaaa tttcgcgcgc gagccaggag cggctgcagg
     1621 ccacggcgcg gctggcgacg gtcggcgaaa tggcttcgct gctgagtcac gagctgaacc
     1681 agccgctggc ggcaatttcc agctacgcga ccggcacgat taacctgctg aagacgcaga
     1741 tggcgtcgca gacgacgtcg gacggcgatg gcggccccac gcccgacatc gaggtggcca
     1801 tgcgccgcat cgccgagcag gccgagcgcg ccggcaaggt gatcaagagc gtgcacgact
     1861 tcgtgcgccg gcgcgacaag gagcgcgagc cggtcgcccc gcaggccctg ctggacgcca
     1921 tcatgccgct ggtcagcctg caggcgcgca agctcggcgt gcgggtcgaa acccgtctga
     1981 tgcgcggcct gcccgatgtc ttttgcgacc gcaccatggt cgagcaggtc ctgctgaacc
     2041 tggcgcgcaa tgcgatgcaa gccatggacc aggtccatgt gaccgatccc tcgctgctga
     2101 tcgaggtgcg ccgcgccacc gccgtgggcg gcgaaacgac gccgggcaag agctggctag
     2161 agttcctggt gaccgatgtc gggcccggca ttcccgagcc ggtcaagcag cagctgttca
     2221 ccccgttctt caccaccaag gccgaaggca tgggcctggg cctgagcctg tgccgcaccg
     2281 tggtggagca gcatggggga ttcctcgggt tcgagcccaa ccgtccgcag ggtacgattt
     2341 tcaggttcac cttgccggtg gacaggtcct agcgcctttc cacccaccag ttcctcgcag
     2401 gacgcgaatg caaccagccg ccaacgccac cgtgttcatc gtcgacgacg acgccagcgt
     2461 gcgcgaggcc ttggcctggc tgctgcggtc gcgccacctg ctcagcgaag gctatgcggg
     2521 cggggccgag ttcgaggcca tgctgcaggg ctacacgccc gcacagcctt gctgcctgtt
     2581 gctggacgtg cgcatgcccg gcatgagcgg gctggcgctg ttcgacaagc tggccgagcg
     2641 cggcctggtc gaggtgatgc cggtgatttt cctcaccggc catgccgacg tgcccacagc
     2701 cgtcgacctg gtcaagcgtg gcgcgttcga cttctgcgaa aagccgtttt ccgacaatgc
     2761 gctggtcgat cgcatcgagc acgccattgg ccgttcccag caggtgctgg cggcgcggcg
     2821 cgagaagctg ctgctgcagg gccgcctggc cgagctcacc gagcgcgagc gcgacgtgat
     2881 gcggctggtg gtcgagggcc tgcccaacaa gttgatcgcc gaccagctcg atatcagcgt
     2941 gcggacggtg gaagtgcacc gcgcgcgtat cttcgacaag atggaagtca agtcggcggt
     3001 cgagctggtg aacgtgctgc gccacgttta gcggcaggcg aagcccgcta cttttcctcg
     3061 tcgtcccgca aaggctcgcc cttcctgcgt cccgaaaaca tcgtccacca gacgatcagc
     3121 accagcagca ccagcgccag cagcgcctcg agcagaatga acagcatgaa ccgcttccta
     3181 tggactgccc ccattgtagg aatgctggtg ggctgcgcga gcgtgccgct gccgcccacg
     3241 gacgaaggtc cggccaggcc cgccgctgag ccgatcggcc ccggcgatac cgggcccttg
     3301 cccccgccgc tgctgcaggg caagagccgg tggcagccgg tgcgctgggc cgagctgccg
     3361 ggctttggcg aggacgccct gcatgaagcc tggaacgcct ggctcaagag ctgcgagcgt
     3421 cccagtcccg cattcgccag ggtctgcccc gaggtgcgtc gcctgtcgat cgccgcggca
     3481 gacgaacagc gcgcctggct gatgcagcgg ctgcagccct accgggtcga gcccctgcag
     3541 gggccggccg agggcctgct gaccggctac tacgagccga tgctcgaggc cagccgccgg
     3601 ccgacggcca cgcaccaggt gccgctgttc cggccgccgg cgaccctggg cagccgcagg
     3661 ccctggtaca cgcggcagga aatcgacacg ctggccgagg cgcgcgccgc cctgcggggg
     3721 cgcgagatcg cctatgtggc cgatccgctg gatgcgctgg tgctgcagat ccagggctcc
     3781 gggcgcgtac gcatcaccga gccggaccgc agtgtgcgca ccgtgcgact ggcttacgcc
     3841 gccagcaatg accagccgta ccgcagcgtg ggcagctggc tgctgcagca gggtgcgatc
     3901 cgcgacgcgt catggcccgg catcaaggcc tgggcgcgcg acaatccgca gcgggtccac
     3961 gagatgctgt ggagcaaccc caggaccgtg ttcttccgcg aggagccgct gggtgagttc
     4021 gacgcagcgt tcggtccgcg cggggcccaa ggcgtgccgc tgacgccggg acgctcgatt
     4081 gccgtggaca aggacagcat tccctacggc acgccggtct ggctggtatc gcgcgggccg
     4141 gtcaccagcc tgcagcgact ggtgctggca caggataccg gcagcgccat cgtcggcgcg
     4201 gtgcgcgcgg actatttcac cggctggggc cccgaggccg gcgagctggc ggggcggctg
     4261 aagcagccac tgcgcctgtg ggtgttgtgg ccgaggtgaa cacggggcag ctggatggcc
     4321 ccacaagacg gcgatgtcgc cgcacaatgg cggcaatctt gtgcgttcag ggggctcgat
     4381 ggcgaaactt ccggtggcag gcgtgttgac cgcggccgtg ttatggggct gtaccgccac
     4441 ggctcccgtt ccatctgcaa gcccggcggc tccgcctggc ccggccgcct cgacggcccg
     4501 ttaccgctgc gaacagaaca tcgaattcac ggtccgtttc gcggaggact cggccatggt
     4561 ggatgcgggg ccgcggggca acgaggtgct gctgcgtgac gcgggcggca ccacgccgct
     4621 gcagacggtc tacaccaatg cgcgcctgcg cgcggaattc ggcctgggcg tgagcgggcg
     4681 cgaggcgatc ctgcgctatc cgttggtgcc gctggtggca cgctgcgtgc ggcaatagcg
     4741 gcgcgctaca gcggccgcgc gctcaggtgc gacagcggca ggctgctgct ggccttgacc
     4801 tcgcccagcg aaaaactggt gtgcaggtcc ttgacgttgg gcaggttgat cagcaccttg
     4861 cgggcgaact gcgaaaaagt gtccaggtcg cgcgaaacca cctgcaactc gaacgtgcct
     4921 gccccgctga tgtagtggca ggagacgatt tccgggatct tgcggatcgc ctcctccatc
     4981 tcgcgggtga gctcgccggt gttgcggttc gcgtccaggc ggacgaaggc cagcacgccc
     5041 agcccgatct tgtggcgatc gatctcggcg tggtagccct tgatatagcc ggcctgctcc
     5101 agggcccgca cgcgccgcca gcagggcgcg gccgacagcc ccacccgctg cgccagttcg
     5161 gcgttggtga ggcgcccgtc ggcctgcaac tccctgagga tggccgcatc gaatttatcg
     5221 agtgtttcca taattccggc attcaagaaa gaatctttct catattagca atagtcgggc
     5281 atagaaagca aagacatatc cgggcggcgg gcatacactt cgcattccgc caaaggcgcc
     5341 cgcttaaccg gcgcgcgcgc ctcgtcaagt ccggcccatc aggctggaat tcctcgacct
     5401 ggagacaaga cgtcaatgaa cgccccgctg cccgaacaca tccgaaaagc gctggaaacc
     5461 gtcacgctgg acgacaagta ctcgctcgac tacggccgcg ccttcatgag cggtgtgcag
     5521 gccctggtca agttgccgat gctgcagcgg ctgcgcgatg cccaggcggg caagaacacc
     5581 gccggcttca tcagcggcta ccgcggctcg ccgctgggcg gctacgacca ggcactgtgg
     5641 aaggccggca agtacctgaa agcccagaac atcgtttttc agcccggcgt caacgaggag
     5701 ctggccgcca cggcgatctg gggtacgcag cagctgggct tcgcgccggc cggcacccgc
     5761 aagttcgacg gcgttttcgg catctggtac ggcaagggac cgggcgtgga ccgcacctcc
     5821 gatgttttca agcatgccaa cctcgcgggg accacgccct ggggcggggt gatcgccgtg
     5881 gccggcgacg accatgtggc caagagctcg acggccgcgc accagagcga ccacatcttc
     5941 aaggcctgcg gcttgccggt gttttttccg gccacggtgc aggaaattct cgacctgggg
     6001 atccatgcct tcgcaatgag ccgtttttcc ggggtctggg ccgggatgaa aacgatccag
     6061 gaaattgtcg agtccagcgc cacggccatg atcgaccccg agcgggtgca ggtcaggatc
     6121 cccaccgact tccagatgcc cccagggggt gtgcacatcc gctggcccga caccgcgctg
     6181 gaacaggaag ctcgcctgtt cgattacaag tggtacgcgg ccctggccta tatccgcgcc
     6241 aaccgcctga actgcaacgt catcgaaagc gcggacgacc gcttcggcct gatcgccagc
     6301 ggcaaggcct acaacgacac gcgccaggcc ttgctggacc tgggcctgga cgacggggct
     6361 tgccgccagc tcggtatccg cctgcacaag gtcggcgtgg tctggccgct ggaagcacag
     6421 ggcacgcgtg aattcgccca gggcttgcgc gaaatcctgg tggtcgagga aaaacgccag
     6481 gtgatcgaat accaggtcaa ggaggagctc tacaactggc gccctgacgt gcggcccaac
     6541 gtcatcggca aattcaacga ggtcgggggc gaccagtccg gcggcgaatg gtcgcagccc
     6601 aatccgatgg ccaacacctt gctgcgcgcc aatgccgacc tgtcgcccgc gctgatcgcc
     6661 cgcgcgatcg cgcaccgcct gaagaagctg ggggtggacg gcgacatggg cgcgcgcatc
     6721 gacgcgcaat tggcggtgct ggaagccaag gagcgctcga tgcaggtgct cgaagtcaag
     6781 gccgaccgcg ccccctggtt ctgctcgggc tgcccgcaca acacctcgac ggtggtgccc
     6841 gagggctcgc gcgccatggc cggtatcggc tgccatttca tggcgacctg gatggaccgc
     6901 gccaccatcg gcttcacgca gatgggcggc gagggtgtgc cctgggttgg ccagcagccg
     6961 ttttccaccg aacagcatgt gttcgccaac ctgggcgacg gcacctattt ccatagcggc
     7021 ctgctggcga tccgccagtc gatcgcggcc ggcgtgaaca tcacctacaa gatcctgtac
     7081 aacgacgccg tcgccatgac cggcggacag caggtcgggg agcggccgga gggccattcc
     7141 gtgaaccaga tcgcgcacag cctgatggcc gaaggcgtga cccgggaggt cgtcgtcacc
     7201 gacgagccgc agaagtacac ggtggccaag ttgccgcccg gtgtcgaggt acaccaccgc
     7261 gacgagctgg accgcatcca gcgggaattc aggcagatca agggcacgac ggccatcatc
     7321 tacgaccaga cctgcgccac cgagaagcgc cgccgccgca agcgcggcgc catggtcgac
     7381 ccggcggttc gcgtggtgat caacgacctg gtctgcgaag gctgcggcga ctgcagcgtc
     7441 aagagcaact gcctgtccgt ggagccgctg gaaaccgagt tcggccgcaa gcgcaccatc
     7501 aaccagagca cctgcaacaa ggacatgagc tgcctgaagg gcttctgccc cagcttcgtc
     7561 accgtcgagg gtgggaacat gaaggtgaaa acaagggcgc aggcgccctt gtccttgggc
     7621 gccttcggcg aactgcccga gccggcgttg ccggccctgg acggtgcgta tgggatcgtc
     7681 gtggccggcg tcggcggaac cggcgtcatc acgatcggcc agctgctggg catggcgggc
     7741 cacatcgaag gcaaaggcat cgtcacgcag gacgcggccg ggctggcgca aaaaggcggg
     7801 gccacctgga gccatgtgct gatcggcgag cggcaggacg acatccgcac cacacgggtg
     7861 agcatggccg gggccgacct ggtcatcggc tgcgacccga tcgtcacggc cggcaaggaa
     7921 acggtgctgc gcatgcgcct gggccgcacc catgtggccc tgaacgccca cagcgcaccg
     7981 accgccgcat tcgtgaagaa cgcggattgg gccaacccgg gcgacgcgtg cgccgccgag
     8041 atcgtcaagg cagtcggccc cgaaggcgtg gccgcgttca atgccgacgc cgcggcgacc
     8101 aagctgatgg gcgacagcat ctataccaac ccgatgatgc tgggctacgc ctggcagaag
     8161 ggctggattc cgctgggccg cgaatcgctg ctgcgtgcga tcgaactcaa cggtgtcgcc
     8221 gtggagaaca acaaggccgc tttcacgtgg gggcggcgcg ccgcccacga cagggccgcg
     8281 gtggaaagcc tgctcgcgcc ggcccaggtc atcgagttca agaagcgcga gacactggaa
     8341 ggcatggtcg agcgacgggc gcaattcctg acggcctacc agaacacggc gtatgcggac
     8401 agctaccgga acttcgtgga caaggtccgg ctggccgaag caccgctggg caaaacggcg
     8461 ctcaccgaag ccgtcgcgcg ctacctgttc aagctgatgg cctacaagga cgagtacgaa
     8521 gtggcccggc tgcatgccga aaccggcttc caggaaaaga ttgccgccat gttcgagggc
     8581 gactacaagg tgaactacca tctcgctccg ccccttgttg ccaggcgtaa cgagaagggc
     8641 gaactgcaga agaaaaagta cgggccggcc atgctcacgg cgtttcgcgt gctggcgaag
     8701 ctcaagggcc tgcgcggcac gccgttcgac atattcggca ccacgtccga gcgccgggcc
     8761 gaaagggcgt tgcccgccga ataccggcag agcatcgaac aggtgattgc gtccctgaat
     8821 gcggccaatc attcgcaggc gctcgagatt gcgcgcattc cggaccagat caaggggttc
     8881 gggcatgtca aggagcgcaa tctggttgcc gcgcgcggcc attggcagca actgatggcg
     8941 cagtggcacg atgcgcaggg cgagcgccgg gctgcctgag ctattttttc gggccggcgg
     9001 acggcgggct tggcgtcggg ttacaagtta ttaccccggc ggcgcggctt acaatcgaac
     9061 gctttccgcc aggcggtgca cgggtcgtgt gccggcttga ctgcgggcgt atttcaacaa
     9121 gatcacccct ggagccgccg tgttcatttc ttccgctttt gcccaaaccg ccgggggcga
     9181 catacagtcg tcactgatga gtatgctgcc gctgatactc atgtttgtgg tgctgtactt
     9241 cgtgatgatc aggccgcaga tgaagcgcac gaaggagcat cgcatgatgg tcgaggcgct
     9301 ggccaagggg gacgaggtgg ccacggccgg cggcatgctg ggcaaggtga ccaagctggg
     9361 cgaggtttac atcggcgtcg aaatcgcgaa cggcatcgaa gtgcaggtcc agcgcagcgc
     9421 ggtggtgcag gtattgccta agggcaccat caagtaatag aaggacacgc ggtgcgcggc
     9481 accggtgttt ttcacctcgt aactatgaac cgatacccgg tctggaaata cgcgatcatc
     9541 gtgatcgcgt tgctgatggg ggcgatctat acgctgccca atttcttcgg cgaggcgccc
     9601 gcggtgcagg tttccccggg caaggcgacg gtcaaggtgg acagcaccac ccgggcccgc
     9661 gtcgagcagg ccctgaaggc ggccggcatc cagccggagg cgatcacgct cgatgccaac
     9721 tccttgaagg tgcggctgga cagcggcgac acgcaattgc gcgcgaagga cgcgatccag
     9781 aaggcgctgg ttcccgaccc cagcgacccg ccctatgtcg tggcgctgaa cctgctttcg
     9841 gcctcgccca agtggctgtc ggcgctgcgc gccgcgccca tgtacctggg cctggacctg
     9901 cgcggcggcg tgcacttcat gctccaggtg gacatgcagg ccgccctgac caagaaggcg
     9961 gagtcgctgg ccggcgacct gcgcaccatc ctgcgcgaga aaaacatccg ccatgccggg
    10021 atcagccgca atggccgggc catcgaggtg cggtttcgtg acacccagac gatggaggct
    10081 gccaaggccg tgatcgtgga ccagttcacg gacctgcaga ccgccgaggt tccggaaggg
    10141 accgagtaca agctcaccgc gaccatcagg cctgaagcgg gccgccgggt gcaggagcag
    10201 gcgctcaaac agaacatggt gacgctgcac aaccggatca acgagctcgg ggtggccgaa
    10261 ccggtgatcc agcagcaggg cctggaccgc atcgtcgtgc agctgccggg cgtgcaggat
    10321 acggccaagg ccaaggacat cctgggccgc accgccacgc tcgaagtgcg catggtcgat
    10381 gaaagcaccg aggcccgcgc ggccgagctg ggcaccggac cggttccctt cggcgacgag
    10441 cgctatctcg agcgcagcgg gcaggccgtc gtggtcagga agcaggtgat cctgacgggc
    10501 gacaacctga cggacgcgca ggcgggcttc gacaaccaga cccaggagcc ggcggtgcac
    10561 ttgacgctgg acgccaaggg tgcgcgcctg ttcaaggacg tgacccggga gaacgtcggc
    10621 aagcgcatgg ccatcctgct tttcgaaaag ggcaagggcg aggtcgtcac cgcgccggtc
    10681 atccgcagcg agatcggggg cgggcgcgtg cagatctcgg gacggatgac cacgaccgaa
    10741 gccaacgacg tcgcgctgct gctgcgcgcc ggctcgttgg ccgcgccgat ggagatcatc
    10801 gaggaactca ccatcgggcc ctcgctgggc gccgaaaaca tcgagaaggg cttccagagc
    10861 gtggcctggg gcttcgccgt gatctgtgcc ttcatggccg tctactacat gctgttcggc
    10921 gtgttctcgg ccgtcgccct ggcggtgaat ctgctgctgc tggtgggcgt gctgtcgatg
    10981 ctgcaggcca cactgacctt gccgggcatg gcggccatgg ccctggcgct gggcatggcg
    11041 atcgattcca acgtgctgat caacgaaaga gtgcgcgagg agttgcgagg cggcgcttcg
    11101 ccgcaagcag cgatcaacac cggctacgaa cgcgcctggg gcacgatttt cgactccaac
    11161 atcaccacca tgatcgccgg catcgcgctg ctggctttcg ggtccggtcc ggtacgcggc
    11221 ttcgcggtgg tgcacgtcat cggaatcctg acctcgatgt tctccgcggt gttcttctcg
    11281 cgcggcctgg tgaacttctg gtatggccgc aagaagaagc tcaagacggt gtcgatcgga
    11341 acggtgtggc gtccggacgg cgatggcgct ataaccaaag ccgagtgacg tttccggaca
    11401 aaggggaata agtggagttt ttcagaattc gccgggacat cccgttcatg cagaacgcgt
    11461 tgatcttcaa cgtgatctcg ttcctgacgt tcgcggccgc cgtgttcttc ctgttcaacc
    11521 gcggactgca cctgtcggtg gaattcaccg gcggcacggt gatggaagtg agctactcgc
    11581 agccggccga cgtcgagaag gtgcgacgca cggtgtccgg gctgggtttc gccgaagtcc
    11641 aggtgcagaa cttcggcaca tcgcgcgatg tgatcatccg cctgcccgcg cagaaaggcg
    11701 tcagctcggc gcaacaaagc gagaaagtgt tggctgcgct gaaggccggc gatccgggcg
    11761 tgagcctgcg ccggacggag tttgtcgggc cgcaggtggg agaggagctg gctacggacg
    11821 gcctgaaggc actggcgatg gtgatcgccg gcatcatgat ctacctggcc atgcgtttcg
    11881 agtggaagtt tgccgtggcg gcgatcatcg ccaacctgca cgacgtgatc atcatccttg
    11941 gcttcttcgc gttcttccag tgggagtttt cgctggccgt gctggccgcc gtgctggcgg
    12001 tgctgggcta ctcggtcaac gaatcggtgg tgatcttcga ccggatccgc gaaaacttcc
    12061 ggcgctaccg caagatgacc acggtgcaga tcatcgacaa cgcgatcacc gccaccatca
    12121 gccgcaccat catcacccat ggctccaccc aggtcatggt gctgtcgatg ctgctcttcg
    12181 gcggcccgac cctgcactac ttcgctctgg cgctgaccat cggcatcctg ttcggcatct
    12241 attcgtcggt tttcgtcgcc gccgccatag cgatgtggct gggcatcaag cgcgaggacc
    12301 tggtcaagtc cgcggcccgc aaggacgagg acccgaacga ccccaacgcg ggcgcgacgg
    12361 tctagcgaga atgagcgttt cccaactgcc acagggctca ggcgcgagga tcgcgcgcca
    12421 ggcgcgcgag cagttcgtgg cgcgcgcgga ggccctcctg ccgcccctgg tccaggcgat
    12481 ccgcagccgg ttggcggagc tggccggcag cggcggcagc tcgcgtcaaa tgcaggagcg
    12541 ccgcgatgcg ttgctggact tcgatcgcaa gggcgccgcc tgggcccagg cgacggccag
    12601 ggtctggcgg caggcgctcg atcctccggc ggcgccaacg cacgtccggc tggaatcggc
    12661 cggcctggag ctgatcggcg acgaggtggt cgaacgcaag atcctgtcgt cacggctggc
    12721 cctggccatc caggaaaaag cgacctggga actcaacgac ctgcggctgc gcatgcagta
    12781 cctcgagggc ggcgatgagc tcgcagccac cgacgtgctg cggcccgagg cgctttcgca
    12841 gttattggtc gagcaatggg acggtgcgga gctttcgcat gaggcgtggg ccctggttca
    12901 cgaggtgatc cagcggcatg tcctggagca tgtgcccgac gcctaccgct ccgtcaacga
    12961 attcctggtg gcccagggcg ttatgcgtga catcgacctg agcagccggg tcaggcgcgg
    13021 cgcgacggcg gctccgggca gaaagtccga cccggctggc ccaggcgccc acgaagcggc
    13081 cggtggtgat gcagatgggg gtttcggcgg cagctgggct tctcgtgatg gtgctgccgc
    13141 ggcggagcgc agcgcaccgg ggggcggtta tggccgtcgc gcggaggctg gcagccaggg
    13201 cggtggccgc gggcaaggcg gtgggcgcgg gcaaggcggt ggccagggcg gcggccaggt
    13261 tggatactcg ggccccgacg gaacgcttca agatggcggc cgctcgcagg cagcccccga
    13321 ctcgagaccg gccggtgctc gcgccggcgg cgtcgccgac gagacccgcc tgatgacttc
    13381 cagcccgcca ctggcccgcg cccggatgcg ggcgaccggc gtgctcggcc aactgaagcg
    13441 gctgctgacc gaacgcgtgg cgggcttcga cggcaatccg ccgacccgtc catcgcccgc
    13501 gttggccgaa gccctgtcgc aacaatccat gccgggccag gccacccggg cgcagggcgc
    13561 cgggacggtg caggaagatt ttgtctacga cgctgccgcc gtgcaggaag tcgcgatcga
    13621 tctgaggcag cggaccagcg agctcaagaa aaaagccacc acgtccaccg agaaggccac
    13681 gatcgagatc gtggcgctca tgttccagag catcctggcc gaggagcgta tcccgccggc
    13741 ggtgcgggtg tggtttgcca ggctgcagat gccggtgctc agagtggcga tcgccgaacc
    13801 cgagtttttc ggctcgctcg aacatccggc gcgccagctg atcgaccgta tggggtcgtg
    13861 cgtgatgggc ttcgacgccg cggccatcgg cggtacggcg ctggagatcg agatcaagcg
    13921 cgtggtccag gtcatcgagc aataccccga aaccggccgc cgcgtattcc agctggttta
    13981 cgacgaattc cagaaattcc tgtccaaatt cctcaccgag acggcgccga cccagcgcct
    14041 ggtcagcgtg gcccagcagg tcgagcagaa ggagaccctg gcgatccagt acacgatcga
    14101 gttgcgcagc atgctggcgg acatgccggt gcgcgatgag atccgcgaat tcctcttcaa
    14161 ggtctgggcc gaagtgctgg cgctgtcagc ggtcaagaac gggccgcagc acgaggacac
    14221 cctggcgctc aagagctcgg cgtccgaact ggtctgggcg gccagcgcca agcccaaccg
    14281 cagcgagcgc gcgcgcgtga tccaggacct gccgcgcctg ttgcagcgcc tgcgccaggg
    14341 catgacgctg gtggggattc agggcaaggc ccaggaagcg cacatcaagg cgatcggcga
    14401 cacgctggcc gatgccttcc tgtccaagac cgaggcgatt ccccacgccc gcatcgagac
    14461 gatggccaag cggctggccc atctggaaga tttcgtcacc gacgacccgg ccaccgacct
    14521 gccgctggac gccgagagca tcgaattgat gctgggtatc gacgcgtcga ccatcgaggt
    14581 ggtggccgat ggaggttcgc ggccgagcgc cgccatgctg gcctgggccg gcgagctgca
    14641 ggtgggtaac tggttctccc tggatcacaa cggcacgatc ttgcaggtgc agtatgcgtg
    14701 gcgcagcgac cgcaagcagt tgcacctgtt cgccgcgcgc ggcggccgca gctacctgat
    14761 ccaggcgcgc cggctggcgg cctatctgca ggccgggctg ctcctgccgg ccgaagagga
    14821 agcgctgacg gtgcgcgcca cgcgcgacgc gctggcgaag ctggacgcaa accccgaacg
    14881 tctattgcag taaacaagca caagttcaag ggccgcatcg cggcccttgt tttgtccgtt
    14941 aatgcggcag gcgcccctgg gcggggtcgc agagctcgtc cagcaccagg gcatcgggct
    15001 cctcgccgaa actccagtac accatcagga tgatgatctt caggtcatcg atcgacaccg
    15061 ggtcacccgg tgcggccatg gcgcggtcga tcacgatctc gcgcatgggc gcggccagca
    15121 cgccggaaga ctcgagaaag gaaacgaagc ccaggcatgg cgcgcccagg tggtcctgtt
    15181 cggccaccga atagacccgc aggctggacg cggattgcag gacggccgcc tccggcgccg
    15241 gtgacgcggt cccgcccaga cgggtgcttt gcgccgccag attcaagcca tcgagccaag
    15301 ccagggcgtc gcgaatctca tcaggctcga acccgtgcgc actgagcttt cgccccagct
    15361 gctgcagctc gggacaggcc tcgccgcgcc agtagtgttc gtagacaaaa acgagcactt
    15421 caaacatgcg ccaatatagc ccagaattcc gccggcggga tgagccgccg gtgtgtgttg
    15481 cgcgccagcc tcagccggcg cccacgcgtt ggaacaagcc gcccggcagg cgcgcgactt
    15541 cgccttgaag ttccagctcc agcagctggg cctgcagttg agccggcgcg actccggtgc
    15601 gtgccaccag tgcatccagg gacacggggt cgaagcccag ggaggcgaga agaccgctct
    15661 cgcgtgagcc gcccgggtgg accggcgcgt tcgccgcgtt tgccgcgcgc tgcgccggcc
    15721 gagcctgcgg caatgcgatc tcctccagca catcctgggc cgtttcgacg agcttcgcgc
    15781 cctgcttgag cagcgcatgg cagccgcgcg attgcggcga atggatcgag cccggaatcg
    15841 cgaagacgtc cttgccctgt tcggcagcca gccgggcggt gatcagcgag cctgaccgca
    15901 gcgcggcctc caccaccaac gtgccctggc tcagggcggc gatgatccgg ttgcgtttcg
    15961 gaaaattggc cgacag
//
LOCUS       DASWIY010000221         5502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1239691, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000221 DASWIY010000000
VERSION     DASWIY010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239691"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1309)
                     /locus_tag="VGA59_08110"
     CDS             complement(50..1309)
                     /locus_tag="VGA59_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9719680.1"
                     /translation="MTSLAGIRVLDLSRVLAGPWCTQTLADLGADVIKIERPGNGDDT
                     RSWGPPFLKDEAGRDTAESAYYLGTNRNKRSVTCDIAQPAGQALIRELVKLCDVFVEN
                     FKVGDMARYGLDHAALQALNPRLVYCSITGFGQTGPYRERAGYDYAIQGMGGLMSVTG
                     ERDGLVDADGRPVAAGPQKVGVAVADLFTGMYASVAILAALRHAERTGEGQFVDMALL
                     DTQVAMLANLGANYLVSGKVPGRAGNAHLNIVPYQVFEVAPAADGGKDHLILAVGNDS
                     QYARFCEIAGRPDLAADPRFAKNQDRVRNRGLLVPMLEPIMKTRGKSEWLAALEAANV
                     PCGAINSIAEVFADPQVNQRGMVTQWRHPLQPALRLVSSPMKLSATPVRSHLPPPLLG
                     EHTEEVLRELLDCSDEQLAQLKNSRVL"
     gene            1563..2750
                     /locus_tag="VGA59_08115"
     CDS             1563..2750
                     /locus_tag="VGA59_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9719681.1"
                     /translation="MVFLAFAFAYFFSALVRAITATLSPTLTREFALSAQELGLLAGG
                     YFFGFAATQLPLGTWLDRYGPKRVILSFLLVAVVGCVAFALATSFTALLAARVLCGVG
                     VSACLMAPLTGYRRWLTPAAQLRANSWMLMTGSFGMLASTLPVQWLVPVMGWRPLFWG
                     LALAVGASMAVIAWQVPRWDAAASVGSEARAGSYAEVWRHPYFRRMSGIGFFNYGGMI
                     AMQTLWAGPWMVRVAGYAPLEAAGGLFIINLSMLATFWSWGMLNPWLARRGLSTDLLI
                     ARGLPLSFVVLATIIIAGPEAGAGAWALFCMSSTFIALAQPAVGMAFPPALAGRALSA
                     YNLVVFAGVFVLQWGIGLLIDGFGALGLSELSSFRLAMAVFLACSVMSYGYFILAKDN
                     SRE"
     gene            2747..3202
                     /locus_tag="VGA59_08120"
     CDS             2747..3202
                     /locus_tag="VGA59_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEX9719682.1"
                     /translation="MNGILIIAHAPLAHALRQCALHVFPDCGPELAAIDVQPNLPPEE
                     TLATARIAMEQLTLGRQIKGVLVLTDIFGATPSNVAQKLVDGVKSRLITGVNLPMLLR
                     SVSYRNEPLEALVSRAVVGGTQGVMQVAITAPQNQPRRNNDQDNYDDQQ"
     gene            3171..3440
                     /locus_tag="VGA59_08125"
     CDS             3171..3440
                     /locus_tag="VGA59_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX9719683.1"
                     /translation="MIRTTTTINNKLGLHARASAKLTKLAGGFPCDIWMSRGDRRVNA
                     KSIMGVMMLAAGIGSEVTVETNGEQEQEAMDCLLALIADRFGEGE"
     gene            3437..5203
                     /gene="ptsP"
                     /locus_tag="VGA59_08130"
     CDS             3437..5203
                     /gene="ptsP"
                     /locus_tag="VGA59_08130"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX9719684.1"
                     /translation="MTFSIHGLPVARGIAIGRAVLMASSRVDVAHYFVEASQVEDEIA
                     RVRTGRNAVVDEIHRLQQTIALMGPKEAPHELTALLDVHLMLLQDEELISGVKHWISD
                     RLYNAEWALTTQLEVLSRQFDDMEDDYLRGRKADLEQVAERILRYMKGSPSPAASHPS
                     ARRKTQQDLLLDDSIDVPLVMIAHDLSPADMLQFKQSVFAGFVTDVGGRNSHTAIVAR
                     SMDIPAVVGARSASQLVRQDDWVIIDGDAGVMIVDPSPIILAEYGFKQRQGELERGRL
                     WRLRHTPAVTLDGQKVELLANIEMPEDASAALKAGAVGVGLFRSEFLFMGRRGNLPDE
                     EEQYQAYRRAVEGMEGLPVTIRTVDVGADKPLDDSMREEAHVNPALGLRAIRWSLADP
                     AMFLTQLRAILRAAAHGQVNMLIPMLSHPGEIRQTVSLIDFAKAELDNRGVAYGPIKL
                     GAMIEIPAAALTLKIFLKYFDFLSIGTNDLIQYTLAIDRADESVAHLYDPLHPAVLRL
                     VAETIAECNAQGKGVSVCGEMAGDVSYTRLLLGLGLRSFSMHPAQILAVKQEVLRADT
                     ARLAPWAREVLDAEDPAQAMAR"
     gene            5235..>5502
                     /locus_tag="VGA59_08135"
     CDS             5235..>5502
                     /locus_tag="VGA59_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719685.1"
                     /translation="MKSVQPRGLRRCAWLIGFLVGILFMTTASVQAGSVLETRGEVWV
                     ERAQQLVPMRAGTALQEGDKLITAPGAEVLVRLEDDARLLVRADS"
BASE COUNT          860 a         1833 c         1851 g          958 t
ORIGIN      
        1 tgaccagccc ggctaccgcg gcgcagctca ggcccaacag ccgcgccgat tagagcaccc
       61 ggctattctt cagctgcgcg agctgctcgt cggaacaatc cagcagttcg cgcagcacct
      121 cttccgtatg ctcccccagc aaaggcggcg gaaggtgcga gcgcaccggc gtcgcgctca
      181 gtttcatcgg actggacacc agccgcagcg ccggctgtag cggatgccgc cactgcgtga
      241 ccataccgcg ctggttgacc tggggatcgg cgaacacctc ggcgatgctg ttgatggcgc
      301 cgcagggaac attggccgct tccagcgcgg ccagccactc gctcttgccg cgcgttttca
      361 tgatcggttc cagcatgggc accagcaggc cgcggttgcg cacgcggtcc tggttcttgg
      421 cgaagcgcgg atcggccgcc agatccgggc ggcccgcgat ttcgcagaac ctggcgtact
      481 ggctgtcgtt gccgactgcc aggatcaggt ggtccttgcc gccatcggct gccggggcga
      541 cctcgaacac ttggtacgga acgatgttca ggtgcgcatt gccggcacgc cccggaacct
      601 tgccgctgac cagataattg gcgcccaggt tcgccagcat cgccacctgc gtgtccagca
      661 gggccatgtc cacgaactgg ccctctcctg tgcgttcggc gtggcgcagt gcggcgagga
      721 tcgccacgct ggcatacatg ccggtgaaca ggtcggccac cgccacgccg accttctgcg
      781 ggcccgcggc caccgggcgc ccgtccgcat ccaccagacc gtcgcgctcc ccggtcacgc
      841 tcatgagccc acccatcccc tggatcgcgt agtcgtagcc cgcgcgctcg cggtagggac
      901 cggtctggcc gaatcccgtg atgctgcaat acaccagccg cgggttgagg gcctgcagcg
      961 ccgcatggtc gagaccgtag cgcgccatgt cgccgacctt gaagttctcg acgaacacat
     1021 cgcacagctt gaccagctcc cggatcagcg cctggcccgc gggctgcgcg atgtcgcagg
     1081 tcacggagcg cttgttgcgg ttggtgccca ggtaataggc ggattcagcg gtgtcgcgcc
     1141 cggcctcgtc cttcaggaag ggcgggcccc agctgcgggt gtcgtcgccg ttgcccggcc
     1201 gctcgatctt gatcacgtcg gcgcccagat ccgccagcgt ctgcgtgcac cagggcccgg
     1261 ccagcacacg ggagaggtcg agaacgcgaa ttccagctaa tgaagtcatg gagcggcatt
     1321 gtcggccaaa tacgcggcaa tacggataat cgggtcatgc tcccgtcctt caccgcggcc
     1381 gtgccggcag gcgttcgcct ccttgtcgcc cgcagtccca actttgcgct tttcgcggga
     1441 cccaagttcg aatgagcggc gcacggtcac ccgaagacgc gcccccgccc gcggggtgcc
     1501 gtgcgctcca cgcggtgacg ggcacgggcg tcgcgtcgct gctttcgcgg cggaccgccg
     1561 tcatggtgtt cctggccttc gcctttgcct atttcttctc ggcactggtg cgcgcgatca
     1621 ctgccacgct ctcgccgacg ctgactcggg agttcgccct cagcgcgcag gagttgggcc
     1681 ttctggccgg cggctatttc ttcggtttcg ccgcgaccca gctgccgctg ggcacctggc
     1741 tggaccgcta tgggcccaag cgcgtgatcc tcagcttcct gctggtcgcc gtcgtcggct
     1801 gcgtcgcgtt tgctctggca accagcttca ccgctttgct ggccgcgcgc gtgctttgcg
     1861 gggtgggcgt gagcgcctgc ctgatggcgc cgctcacggg ctatcggcgc tggctcacac
     1921 ccgccgccca gctccgggcc aattcatgga tgctgatgac cggctccttc ggaatgcttg
     1981 cgtccacgct gccggtgcaa tggctggtgc cggtgatggg ctggcggccg ctgttctggg
     2041 ggctggcgct ggcggtcggc gcctcgatgg ccgtcatcgc ctggcaagtg ccgcgctggg
     2101 acgccgccgc ctcggtgggg tcggaagcca gggcgggcag ctacgccgag gtctggcggc
     2161 atccttactt ccgccggatg agcgggatcg gttttttcaa ctacggcggg atgatcgcga
     2221 tgcagacctt gtgggccggc ccgtggatgg tgcgcgtggc cggctacgcg ccgctggagg
     2281 cagccggcgg cttgttcatc atcaatctga gcatgctcgc caccttctgg agctggggca
     2341 tgctcaaccc ctggctggcc cgcagggggc tgagcaccga cctcctgatc gcccggggct
     2401 tgcccctcag tttcgtcgtg cttgctacaa taatcatagc gggaccggag gccggagcgg
     2461 gagcgtgggc gcttttctgc atgagttcga ccttcattgc cctggcccag ccggcggttg
     2521 gcatggcgtt tccgccggcg ctggccgggc gcgccctgtc ggcctacaac ctcgtggtct
     2581 ttgccggcgt gttcgtgctg caatggggca tcgggcttct catcgacggt ttcggcgcac
     2641 tgggcttgag tgaactgtcg tcttttcggc tcgccatggc cgtgtttcta gcctgcagcg
     2701 ttatgtccta cggatacttc atcctcgcaa aggataattc ccgggaatga acgggatatt
     2761 gatcatcgcc cacgcaccgc tggcgcatgc gctgcggcag tgcgcgctgc atgtctttcc
     2821 cgactgcggc ccggaactgg cggcgatcga cgttcagccc aacctgccac ccgaggaaac
     2881 gctggcaacg gcccggatcg ccatggaaca gctcacgctg ggtcggcaga tcaaaggcgt
     2941 cctggtgctc accgacattt tcggcgcgac gcccagcaac gtggcgcaaa agctggtgga
     3001 cggcgtgaaa tcgcgcctga tcaccggcgt gaacctgccc atgctgctgc gcagcgtgag
     3061 ctaccgcaac gaaccgctgg aagccctggt ctcgcgcgca gtggtcggcg gcacccaggg
     3121 cgtgatgcag gtggcgatca ccgcgccgca gaaccagccc cgaagaaaca atgatcagga
     3181 caactacgac gatcaacaat aagctcggcc tccatgcccg ggcatcggcc aagctgacca
     3241 agctggccgg cggctttccc tgcgacatct ggatgagcag gggcgaccgg cgcgtgaacg
     3301 ccaagagcat catgggcgtg atgatgctgg ccgccggcat cggcagcgaa gtgaccgtcg
     3361 aaaccaacgg cgagcaggag caggaggcaa tggattgcct gttggcgctg atcgcggaca
     3421 ggttcgggga gggggaatga ccttttcgat ccacggcctc cccgttgccc gaggcatcgc
     3481 tatcgggcgc gcggtgctga tggcctccag ccgcgtcgac gtggcgcatt actttgtcga
     3541 ggcctcgcag gtcgaggacg agattgcgcg cgtgcggacc ggacgcaatg ccgtggtcga
     3601 cgagatccac cgcctgcagc agacgatcgc gctgatgggg cccaaggaag cgccgcacga
     3661 gctgacggcc ctgctcgatg tccacctcat gctgctgcag gacgaggagc tgatcagcgg
     3721 cgtcaagcac tggatcagcg accgcctcta caacgccgaa tgggcgctga cgacgcagct
     3781 cgaggtcctg tcgcgccagt tcgacgacat ggaggacgac tacctgcgcg ggcgcaaggc
     3841 cgacctcgag caggtcgccg agcgcatcct gcgctatatg aagggctcac cttcgccggc
     3901 ggcgtcgcac ccgtcggcac gccgcaagac ccagcaggac ctgctgctgg acgacagcat
     3961 cgacgtgccg ctggtgatga tcgcgcacga cctgtcgccg gccgacatgc tgcagttcaa
     4021 gcagagcgta tttgccggct tcgtcaccga cgtgggcggc agaaactcgc atacggcgat
     4081 cgtggcgcgc agcatggaca tcccggccgt ggttggcgcc cgcagcgcca gccagctggt
     4141 gcgccaggac gactgggtca tcatcgacgg cgacgccggc gtgatgatcg tcgatccttc
     4201 gccgatcatc ctggccgagt acggcttcaa gcagcgccag ggcgaactgg agcgcgggcg
     4261 gctctggagg ctgcgccaca cgccggccgt cacgctcgac ggccagaagg tcgagctgct
     4321 ggccaatatc gagatgcccg aggacgcgtc ggccgcgctg aaggcgggcg ccgtcggcgt
     4381 cggcctgttc cgcagcgaat tcctgttcat gggacgccgc ggcaacctgc cggacgagga
     4441 ggagcaatac caggcgtacc ggcgcgcggt cgagggcatg gaggggctgc ccgtgacgat
     4501 ccggaccgtg gacgtgggcg ccgacaagcc gctggacgac tcgatgcgcg aggaggccca
     4561 cgtgaacccg gccctgggcc tgcgcgcgat ccgctggagc ctggcggacc cggccatgtt
     4621 cctgacgcag ctgcgggcga tcctgcgcgc cgcggcccac ggccaggtca atatgctgat
     4681 cccgatgctg tcgcatcccg gcgaaattcg ccagacggtg tccctgatcg atttcgccaa
     4741 ggccgaactg gacaaccgcg gcgtcgctta tgggcccatc aagctcggcg cgatgatcga
     4801 gattccggcg gcggcgctga ccctgaagat cttcctgaaa tacttcgact tcctgtcgat
     4861 cggcaccaac gatctgatcc agtacacgct ggccatcgac cgcgcggacg aatcggtggc
     4921 gcacctgtac gacccgttgc atcccgccgt gctgcggctg gtggccgaaa ccattgccga
     4981 atgcaatgcg cagggcaagg gggtcagcgt ctgcggcgag atggcggggg atgtgagcta
     5041 cacgcgcttg ctgctgggcc tgggcttgcg cagtttctcc atgcatcccg cgcagatcct
     5101 tgccgtcaag caggaggtgc tgcgagccga tacggcccgg ctggcgccct gggcacgtga
     5161 ggtgctcgac gccgaggacc cggcacaggc gatggcgcgc taaccgtgga tactttcaaa
     5221 gaggcgggtt ccgaatgaaa tctgtccagc cccgtggact gcgccgctgt gcctggctga
     5281 tcgggtttct cgtcggtatc ctgttcatga cgacggcatc cgtgcaggcc ggctccgtcc
     5341 tcgagacgcg cggggaggta tgggtcgagc gcgcgcagca gctcgtgccg atgcgcgcgg
     5401 gtacggcgct tcaggagggg gacaaactca tcaccgcccc gggcgccgag gtcctggtgc
     5461 gcctcgaaga cgacgcccgc ctgctcgttc gcgccgattc ca
//
LOCUS       DASWIY010000222         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3109212, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000222 DASWIY010000000
VERSION     DASWIY010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3109212"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VGA59_08140"
     CDS             complement(<1..176)
                     /locus_tag="VGA59_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX9719686.1"
                     /translation="MKLVTAIIKPFKLDEVREALSGIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKV"
     gene            complement(206..943)
                     /locus_tag="VGA59_08145"
     CDS             complement(206..943)
                     /locus_tag="VGA59_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX9719687.1"
                     /translation="MELKLTQKMVLAILALSGAAFAQTKAPEPDYTLSYNVGVVSDYR
                     YRGISQSRLRPALQGGADFAHKSGFYLGAWASTIKWIRDAGGDARVEVDLYGGYKGTA
                     GAFGYDVGVLRYQYPDHSLAVSPNTTEIYGAGSFGPATLKYSHAVTNLFGFSDSKNSY
                     YLDASAGFDLGSGWSVTPHIGYQKVKNNSDFSYTDYAVTVGKDFGNGLSASAAVVATD
                     ADDALYVTPAGKFTGKTGLVVGVKYSF"
     gene            1154..>2296
                     /locus_tag="VGA59_08150"
     CDS             1154..>2296
                     /locus_tag="VGA59_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX9719688.1"
                     /translation="MSLSLVQSRALVGLQAAAVTVEVHLANGLPSFTLVGLADVEVKE
                     ARERVRSALQNCGLEFPHNKRITVNLAPADLPKDSGRFDLPIALGILAASGQVDAARL
                     AGYEFAGELSLSGELRPVRGALAMSLALHGAGATARLVLPPGSAEEAALVPQAQVYRA
                     DHLLDVVRQFLPSQDAGDSDGWARVVAQPAAARASYPDLADVKGQAGAKRALEIAAAG
                     GHSLLLLGPPGSGKSMLAQRFAGLLPAMTVDEALQSAAIASLAGQFSQERWGLRPTCS
                     PHHTASAVALVGGGSPPRPGEISLAHHGVLFLDELPEFPRAALEALREPLETGQITIS
                     RAARRAEFPARFQFIGAMNPCPCGYRGSPHKACRCTPDQVARYQGKL"
BASE COUNT          364 a          757 c          786 g          389 t
ORIGIN      
        1 accttgggca ggaagtccac cacgtactcc gcgccgcggt acagctcggt gtggcccttc
       61 tggcgcccga agcccttgac ctcggtgacg gtgatgccct gcaccccgat gccggagagc
      121 gcttcgcgca cctcgtccag cttgaatggt ttgatgatgg ccgttaccag tttcatgtct
      181 tctctccttg agaatcgcga atcgatcaga agctgtactt cacgcccacc accaggcccg
      241 tcttgccggt gaacttgccg gcgggcgtga cgtagagcgc atcgtccgca tcggtggcga
      301 cgacggcggc actggccgac aggccattac cgaagtcctt gcccaccgtc accgcgtagt
      361 cggtgtacga gaaatccgag ttgttcttga ccttctggta gccgatgtgc ggcgtgacgg
      421 accagccact gcccagatcg aagccggcgc tggcatccag gtaatagctg ttcttgctgt
      481 cactgaaccc gaacagattg gtgacggcgt gcgagtactt cagcgtggcg gggccgaacg
      541 agccggcgcc gtagatttcg gtcgtgttgg ggctgacggc caggctgtgg tccgggtact
      601 ggtatctcaa cacgccgacg tcgtagccaa aggcgccggc cgtgcccttg tagccgccat
      661 agaggtcgac ttcgacccgt gcgtcgccgc cggcgtccct gatccacttg atggtcgacg
      721 cccaggcgcc cagatagaag ccgctcttgt gcgcgaagtc ggcgccgcct tgcagggccg
      781 ggcgaaggcg cgactgcgaa atgccccggt agcggtagtc actgaccacg cccacgttgt
      841 acgagagcgt gtaatccggc tccggcgctt tggtctgggc aaaggcggcg cccgacaggg
      901 cgaggattgc cagaaccatt ttttgggtca acttgagttc caccgagttc tcctgagtaa
      961 agggggttga gctcagcgta agcaggtagc gtgccaaaag tcacaagaac cctggcacgg
     1021 tgcacggagc ctgtccatgc ggcgttatgt cattgcgccg tgtaacgatg agcagagtgc
     1081 accggcgtgg tgccatgtgg aacatttccc gcaccacacc tgcacatgca cctgaatggg
     1141 gaggagatct tcgatgagct tgtctttggt gcaaagccgc gccctggttg ggctgcaggc
     1201 ggccgccgtc actgtcgagg tgcatctggc caatggcctg cccagcttta ccctggtcgg
     1261 gctggccgac gtcgaagtca aggaggcgcg cgaacgcgtg cgctccgcgc tgcaaaactg
     1321 cgggctggag tttccccaca acaagcgcat cacggtcaat ctcgcgccgg ccgacctgcc
     1381 caaggattca ggccgcttcg atctgccgat cgcgctgggc atcctggccg ccagcgggca
     1441 ggtggacgcg gcccggctgg cgggctatga gttcgcgggc gaactatcgc tgtcgggaga
     1501 attgcggccg gtgcgcggcg cactggcgat gagcctcgcg ctgcacggcg cgggtgccac
     1561 ggccaggctg gtgctgccgc cgggcagcgc ggaagaggcg gcgctggtgc cgcaggcaca
     1621 ggtgtaccgg gccgaccacc tgctggacgt ggtgcgccag ttcctgccgt cgcaggacgc
     1681 cggagattcc gacggctggg cgcgcgtggt cgcgcagccg gcggcagccc gcgccagcta
     1741 cccggacctg gccgacgtga aaggccaggc cggcgccaag cgggccctgg aaatcgccgc
     1801 ggcgggcggc cacagcctgc tgctgctcgg cccgccgggc tcgggcaaat cgatgctggc
     1861 gcaacggttc gcgggcctgc tgccggcaat gacggtggac gaggcgctgc aaagtgccgc
     1921 catcgccagc ctggccgggc agttctcgca ggagcgctgg gggctgcggc cgacctgcag
     1981 cccgcaccac acggccagcg cggtggccct ggtgggcggg ggttcgccgc cccggcccgg
     2041 cgagatttca ctggcgcatc acggcgtgct gtttctcgac gagctgcccg agtttccgcg
     2101 cgccgcgctg gaagccctgc gcgagccgct ggagaccggc cagatcacga tctcgcgcgc
     2161 cgcgcggcgg gccgaattcc cggcccgctt ccagttcatc ggcgcgatga acccctgccc
     2221 ctgcggctat cggggctcgc cccacaaggc ctgccgctgc acgcccgacc aggtcgcgcg
     2281 ctaccagggc aaactc
//
LOCUS       DASWIY010000223         4716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2003061, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000223 DASWIY010000000
VERSION     DASWIY010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003061"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VGA59_08155"
     CDS             <1..370
                     /locus_tag="VGA59_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX9719689.1"
                     /translation="PGLHAGAFTLGLQTRPDQAAQAVRVSREVLARFVTDGPTEAELK
                     AAKDYLIGGFPLLIDSNRKLLDNIANIAWHGLPLDYLDTWTKQVERVTVADVKAAFAR
                     KLQPQRMVTVTVGAVAAAQQ"
     gene            611..1201
                     /gene="rsmD"
                     /locus_tag="VGA59_08160"
     CDS             611..1201
                     /gene="rsmD"
                     /locus_tag="VGA59_08160"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX9719690.1"
                     /translation="MTRHIKPPSHSRAANEVRIIGGQWKRTRLPVADKPGLRPTPGRV
                     RETLFNWLGQDLTGRRCVDAFAGTGALGLEAASRGAAEVLLVEQDAQLVSQLGAVQAK
                     LGAAAVKVERANGLAVLGRLAAGSIDLIFLDPPFDADLFGKAIAAAAAALADEGLIYL
                     EAPVAWDDAKLAPYGLTVYRHLRAGAVHAHLLGRAA"
     gene            1253..1753
                     /gene="coaD"
                     /locus_tag="VGA59_08165"
     CDS             1253..1753
                     /gene="coaD"
                     /locus_tag="VGA59_08165"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX9719691.1"
                     /translation="MASNVIAVYPGTFDPMTLGHEDVVRRATQLFDRVIVAVAAGHHK
                     QSMFTLQERIQMARDVAQAYAQVEVESFAGLLRDFVVARGAKAMVRGLRAVTDFDYEF
                     QLAGMNRSLMPDVETVFLTPSDKYQFISSTFVREIALLGGEVDKFVSPSVQVQLMEKV
                     RSLGRE"
     gene            1886..2560
                     /locus_tag="VGA59_08170"
     CDS             1886..2560
                     /locus_tag="VGA59_08170"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9719692.1"
                     /translation="MTELILLRHGETDWNRELRFQGHVDVALNAIGLEQARRLARRLA
                     HETAHGLYASDLLRAKQTALPVAQQLGLASVSDPALREQSFGLVDGMKVDDIKAQHPR
                     AWEGWLRFQEDFSMPEGESTRQFHARVMDAVHRLVAAHPAETLVVVTHGGVLDMIYRT
                     ARSLGLGGPRQSEIPNAGLNRVRVHEGGIDILTWADVQHLHDLPPQPVYDQLKLLATA
                     SRPSAA"
     gene            complement(2515..3858)
                     /locus_tag="VGA59_08175"
     CDS             complement(2515..3858)
                     /locus_tag="VGA59_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9719693.1"
                     /translation="MPQRTDCLGNDVTLENPGSLGALNDFVEGFIASEARAMNILEAA
                     AEDTSAIVQACAAAVHMFAESAAAAANARPFIDRALASEGRCTERERRFVHAVQAWVD
                     GDILRAIALHEEQAREHPRDLASLKLGHYHLFNRGDSPGMLRMALAALPAAGDVPYLH
                     GMAAFAWEQCHLLGEAEAAARKAIGMCRKEPWAHHALAHVMLTQGRIHEGHAFLHDVS
                     NTWTALNSFMVTHNWWHQALFALELERHDEVLGLYDREVWGVVKDYSQDQVNAVSLLA
                     RLELAGVDVGERWQDVGNYLAGRLQDHVLPFLDMHYLYGLARAGRAEADTLMGNIERY
                     AARAPEDARTAWQRVCVPAGRGLLAHARGDDARAADELGQALPRLAEIGGSHAQRDLF
                     AQVRLDAMIRSGQLVGAQQQLQLQLAGQPESARLKRQAAEVYAALGLEAVARSFS"
     gene            complement(4143..4547)
                     /locus_tag="VGA59_08180"
     CDS             complement(4143..4547)
                     /locus_tag="VGA59_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9719694.1"
                     /translation="MNPVVHFELPYVNRERIARFYESAFGWQTEKLGEEMGNYVLATT
                     ARSDAKPGAPAGAINGGFYPQKPDWPAQHPSVVIAVPDLDAGMARVNSAGGKVLGDPM
                     DIPGVGRYVAFFDTEENRLSMLQPSAGVAGDS"
BASE COUNT          799 a         1556 c         1578 g          783 t
ORIGIN      
        1 gcccgggctg catgcggggg cgttcaccct gggcctgcag actcggcccg accaggcggc
       61 gcaggcagtg cgggtttcgc gcgaggtgct ggcgcggttc gtaaccgacg ggccgaccga
      121 agcggagctc aaggccgcga aggactacct gatcggcggc tttccgctgc tgatcgacag
      181 caaccgcaag ctgctggata acatcgccaa tatcgcctgg cacggcctgc cgctggacta
      241 cctggacacc tggacgaagc aggttgaacg ggtgaccgtt gccgacgtca aggccgcttt
      301 cgcgcgcaag cttcagcccc agcgcatggt caccgtcacg gtgggcgccg tcgcggcggc
      361 ccaacagtag aaacgcaaaa acgcggccgc acctgtcaat gttgacaggg gtcggcaaca
      421 cggcaaatct tttcgcctcc agaatggtct atcgctgtca cgcgagaggt agcgccaggg
      481 gggcgagaaa gagggacagg caggacccgc acccgcagca tcgagctgcg ggtgcgccgg
      541 tcctgttgcg tctttatcat gtgcatgccg ttgcggccat tgcgaggtgt cgccgccgcc
      601 acggattcac atgacgcgac acatcaaacc tccatcgcac agccgcgccg cgaacgaagt
      661 gcggatcatc ggcggccaat ggaaacgcac caggctgccc gttgccgaca agccgggact
      721 gcggcccacg cccggccgcg tgcgcgaaac cctgttcaac tggctgggcc aggacctcac
      781 cggccggcgc tgtgtcgatg cgtttgccgg caccggcgca ctcgggctgg aggcggcttc
      841 gcgcggggcg gccgaggtgt tgctggtgga acaggacgcg caactggtgt cccagctcgg
      901 cgccgtgcag gcaaagctgg gcgccgccgc ggtgaaggtg gagcgcgcca acggcctcgc
      961 cgtgctgggc cggctggccg ccggcagcat cgatctgatc tttctggatc cgcccttcga
     1021 tgccgacctg ttcggcaagg ccatcgccgc ggcggcggcg gcactggccg atgaagggct
     1081 gatctacctc gaagcgccgg tggcctggga cgacgcaaag ctggcgccct acggcctgac
     1141 cgtgtaccgg catctcaggg cgggggccgt gcacgcccac ttgctggggc gggctgcata
     1201 atgcactgca gcaaacggtc gaaaccggcc gttgcctgtt gaggagacat ccatggccag
     1261 caacgtgatc gccgtctatc ccggcacttt cgatccaatg accctgggcc atgaagacgt
     1321 tgtccgccgg gccacccagc tgttcgaccg cgtcatcgtc gcggtggcgg cgggccatca
     1381 caagcaatcc atgttcacgc tgcaggagcg catccagatg gcgcgcgacg tggcgcaggc
     1441 ctatgcgcag gtcgaggtgg agagctttgc aggattgctg cgcgatttcg tcgtggcgcg
     1501 cggcgccaag gccatggtgc gcggcttgcg cgccgtgacc gacttcgatt acgagtttca
     1561 gctcgcgggc atgaaccgca gcctcatgcc ggatgtcgaa acggtattcc tcacgcccag
     1621 cgacaagtac cagttcatca gcagcacctt cgtgcgggag atcgcgttgc tgggcggtga
     1681 agtggacaag ttcgtctcgc cgtcggtgca ggtccagctg atggagaagg tccgcagcct
     1741 gggccgcgag taaatgcggc caattgcgcc tgccacatga cggcatcgcc cgccaggccc
     1801 ggcaacaggc tcgatcgggt catgaataag atttcattgg gtaaatatcc attctgtcat
     1861 gcggacaggc tgggataatc ggcagatgac agaactgatc ctgctgcgcc acggcgaaac
     1921 cgactggaat cgcgagcttc gcttccaggg ccatgtggac gtggcgctga acgccatcgg
     1981 gctggagcag gcgcgccgtc tcgcgcgccg gctggcgcac gagacggcgc atggcctcta
     2041 tgcgagcgac cttctgcggg cgaaacagac cgcgcttccg gtcgcgcagc agctcggcct
     2101 ggcgagcgtc agcgacccgg cactgcgcga gcagagtttc gggcttgtcg acggcatgaa
     2161 ggtcgacgac atcaaggcgc agcatccgcg ggcgtgggaa ggctggctgc gattccagga
     2221 ggatttcagc atgccggagg gcgaaagcac ccggcagttc cacgcgcgcg tcatggacgc
     2281 ggtgcaccgt ctggtggccg cgcaccccgc cgaaaccctg gtggtggtca cgcacggcgg
     2341 tgtgctggac atgatctatc gcacggcgcg ttcactcggc ctgggcgggc cgcgccagag
     2401 cgaaatcccg aacgcgggcc tgaaccgcgt ccgggtccat gaaggcggca tcgacatcct
     2461 gacctgggcc gacgtgcagc acctgcacga cctgccaccc cagccggtct acgatcagct
     2521 gaagcttctg gctaccgctt ccaggccgag cgccgcatag acctcggcgg cctggcgttt
     2581 caggcgagcc gactcgggct ggcctgccag ttgcagttgc agctgctgct gcgccccgac
     2641 gagctggccg cttcgaatca tcgcgtccag ccgcacctgc gcgaacaggt cgcgttgcgc
     2701 atggctgccg ccgatctccg cgagccgcgg cagcgcctgt cccagttcgt ccgccgcgcg
     2761 cgcgtcgtcg ccgcgggcgt gcgccagcag gccgcggccg gccgggacgc atacgcgctg
     2821 ccacgcggtg cgggcatcct ccggcgcgcg tgccgcgtac cgctcgatgt tgcccatcag
     2881 ggtatcggcc tcggcccggc cggcccgtgc caggccatag aggtagtgca tgtccaggaa
     2941 cggcagcacg tggtcctgca ggcggcctgc caggtagttg cccacgtcct gccagcgctc
     3001 gcccacgtcc acaccggcga gttcgagcct tgccaataag gacactgcgt tgacctgatc
     3061 ctgcgagtaa tccttcacca cgccccacac ttcgcggtcg tacaggccca gcacctcgtc
     3121 atgacgctcc aactccagcg cgaacaaggc ctggtgccac cagttgtgcg tcaccatgaa
     3181 cgaattcagc gcggtccagg tgttgctcac atcgtgcagg aaggcatggc cttcatggat
     3241 gcgtccctgg gtcagcatca cgtgcgccag cgcatggtgg gcccacggtt ccttgcggca
     3301 catgccgatc gcttttcgcg cggctgcctc ggcctcgccc agcaggtggc attgctccca
     3361 cgcgaaggcg gccatgccgt gcaggtaagg cacgtcgccc gcggccggca gggccgccag
     3421 cgccatgcgc agcatgccgg gcgaatcgcc ccggttgaac aggtggtaat gaccgagctt
     3481 gagggaagcg aggtcgcgcg gatgctcgcg ggcctgctct tcgtgcaagg cgatggcgcg
     3541 caggatgtcg ccgtccaccc acgcttgcac ggcgtggacg aagcggcgct cccgctcggt
     3601 gcatcgccct tcggatgcca gcgcgcggtc gatgaaagga cgggcattgg cggcagccgc
     3661 ggccgattcg gcgaacatgt gcaccgccgc cgcgcaggcc tggacaatgg cgctggtgtc
     3721 ctcggccgcc gcttccagga tgttcatcgc gcgtgcctcg ctggcgatga agccctcgac
     3781 gaaatcgttc aacgcgccga gactccccgg gttctcgagc gtgacgtcat tgccgaggca
     3841 gtcagttctc tgcggcatag gccctgttca cactgttttt gccagtgcga gtggggtgag
     3901 aaccgcgcca atcgaggcgc gggacgacgg caaggctggt cgccttgcct aggagcgcaa
     3961 cgccgagtgg cgcggttctc acccccctcc ctgcgggttg cgattccgaa ggggccatcc
     4021 gcgttgttac ggaacacggc tgggtgtgca acccaggccg tgttccgcgc ctagcggctg
     4081 accccttctg atcgcaacgc atctggcaaa aacagtgtga acaggccctg atgcccgctt
     4141 cttcaggaat cgcctgccac gccggccgag ggctgcagca tgctgaggcg gttttcctcc
     4201 gtatcgaaga acgccacata gcggcccacg ccgggaatat ccatggggtc gcccagcacc
     4261 ttgccaccgg cggagttcac acgcgccatg cccgcatcca ggtccggcac cgcgatgacg
     4321 acggacgggt gctgcgcggg ccagtcgggc ttttgcggat aaaagccccc gttgatggcg
     4381 cccgccggcg cgccgggctt ggcgtcgctg cgcgccgtcg tggcgagcac atagttgccc
     4441 atttcctcgc ccagcttctc ggtctgccag ccgaaggcgg attcgtaaaa gcgggcgatg
     4501 cgctcgcggt tcacgtaggg aagctcgaag tggaccacag gattcatgac acggctcctt
     4561 tcaacgcccg tcggcggcac gctcgagctg accgatatcg agtttcttca tcttcagcat
     4621 caacggccgg gcaacagtta accatgtggt ttagtatatc gacgggcaat ggcctgtcaa
     4681 gaaggtggag ataaccgaaa gtaactggcg cggccg
//
LOCUS       DASWIY010000224         8680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_516130, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000224 DASWIY010000000
VERSION     DASWIY010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516130"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VGA59_08185"
     CDS             complement(<1..125)
                     /locus_tag="VGA59_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX9719695.1"
                     /translation="MKVLVPVKRVVDFNVKVRVKSDNTGVDIANVKMSMNPFDEIA"
     gene            complement(384..1646)
                     /locus_tag="VGA59_08190"
     CDS             complement(384..1646)
                     /locus_tag="VGA59_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX9719696.1"
                     /translation="MTELDKLVRDLGHPGVPWELGVLLGCLALAFGVCWLVGRRHSSD
                     SVWFGRATVDGLLFPLVALVLTYSAMLVLAQRQPVALLRVAVPVLISLAGIRFLARVL
                     TVVFPASRFARLVERLFSWLAWMAAVLWIVGLLPAVMDEMEAIHFVFGKSKVSLLSLV
                     QGLLSSGVVLVLALWISAALEKRILRETVDDLSLRKVAANAIRALLLLVGLLFALSAT
                     GVDLTALSVLGGAIGVGLGFGLQKLAANYVSGFVILFERSLRIGDTVRVDQFEGTVMD
                     IKTRYTLIRSLSGRESIVPNEKMITERIENLSLADPRILLTTDVTVGYDSDVDRVQQI
                     LVGAAVGTDRVIADPPPGARLARFGADGLEFTLLFWIADPSNGQLNVKSDVNLRVLKG
                     LREAGIDIPYAQRVVHLKTASSATASTG"
     gene            complement(1662..2438)
                     /locus_tag="VGA59_08195"
     CDS             complement(1662..2438)
                     /locus_tag="VGA59_08195"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9719697.1"
                     /translation="MDEALIEKKDERGVVTLTMNRAGAFNALGEEMIDALQAALDRVA
                     KDASARVVVLAAAGKAFCPGHNLKEMIAKPSLEYYQRLFAQCSRMMLGIQKLPVPVIA
                     KVQGIATAAGCQLVAQCDLAVASSAASFAVSGVNFGLFCSTPSVPLLRNIAPKQAMEM
                     LVTGEFITAQQALERGLVNRVCPPEQLDAEVEKLVASILGKPRTAIAIGKELFYRQRE
                     MGIEAAYQLASQTMAVNMMEGCAQEGVTAFTEKRKPAWKS"
     gene            complement(2467..3420)
                     /locus_tag="VGA59_08200"
     CDS             complement(2467..3420)
                     /locus_tag="VGA59_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX9719698.1"
                     /translation="MNKTGYYTHPDCRRHEMGPGHPECPERLDAIEDRLIASGVGLAL
                     ERRQAHEASPADIELAHTRMHVAALRGLDQELDEHIAAGGRGYAQIDPDTLMNPDTWK
                     AALRAAGAALSATDAVIAGELENAFCAVRPPGHHACRDRAMGFCFFNNIAIAAKYALE
                     RHGLKRVAIVDFDVHHGNGTEDILGGDERALMVGFFQHPFYPYTGTEGHHPNMVNLPV
                     PAYTRGMAIRELVEQAWMPRLADFRPEMIFISAGFDAHREDDLGQLGLVEADYTWITM
                     KVKEIAKKYASGRIVSSLEGGYNLSALGRSVEAHLRVLADI"
     gene            3466..4386
                     /locus_tag="VGA59_08205"
     CDS             3466..4386
                     /locus_tag="VGA59_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9719699.1"
                     /translation="MDAQQKLQALLDQLNTVIVGKPVQVRDCVACLLAGGHLLIEDVP
                     GVGKTTLAHALSRALGLHFSRVQFTADLMPSDLSGVSIYERGKEAFVFHPGPVFAQVL
                     LADEINRASPKTQSALLEAMEEKQVTVEGETRALPTPFFVIATQNPHDQLGTFALPES
                     QLDRFLMRISLGYPDRAAERELLNGADRRGMVDAMRSTFTPVDLLQLQQKVLQVHAAG
                     PLLTYVQDLVAATRSGRWFLQGLSPRAGIAVVRAAKAQALLSGRDYVAPDDVQAILPQ
                     TVAHRLIPVGDAGRGPIEQVRAMLEAVPLP"
     gene            4521..5498
                     /locus_tag="VGA59_08210"
     CDS             4521..5498
                     /locus_tag="VGA59_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9719700.1"
                     /translation="MNAGVLDPVTFVRRQFRRWWQARLPLADTIELTQRNVYILPTRP
                     GVMLGLTLVVLLVGSINYQLNLGYLLTFLLAGSAVVGMYVCHGTLRGLTMNLVPPDPQ
                     FVGASATLSIVLTNDRRSTRFGIGLAVLEATHEDRWVWTDVPPLGQSTVQVAFKPERR
                     GLHRVPPLTAETRFPLGTFRVWNVWRPAAQVLAYPAPETFPPPLPPGEPRAGGAASAR
                     VQSTGEFDGVRAYRRGDPLKLVVWKKAAKADELVSRDNEQAQRYELWLDYAQAGGLGA
                     EHTLSRLTAWVLQAERLGLDYGLRLPGQEIAAATGEAHKRRCLEALATC"
     gene            5492..7501
                     /locus_tag="VGA59_08215"
     CDS             5492..7501
                     /locus_tag="VGA59_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEX9719701.1"
                     /translation="MLSRLNALPRDSRDTLFLLLVIGWVILPQVAKLPLWCSALAGAV
                     LVWRGWLAVASRPLPGKWWLLGLLAVTVAATFATHRTLLGRDAGVTLIVVLLALKTLE
                     LRARRDAFAIFFLGFFTMLTNFFFSQSLPTAAAMLVALLGLLTALVNAHMPVGRPPLA
                     QAARTAGWMALLGAPIMAVLFVLFPRLAPLWGIPSDAMSGRSGLSASMQVGSMASLAL
                     DETIAMRVKFDGPPPSQGDLYFRGPVLSAFDGREWRPLQSRLGSPFSPVVPGTAQLDV
                     LGEPVRYEVTLEPNNRPWVLVLDAAMKTPTVPGMRAVMTSELQWVANRPVTDLLRYGA
                     ESHLVFRHGPRTSAAAVPPQYIALPPGFNPRTLALADELRREPRLAAGGPAALVQAAL
                     DRLRTGNYQYTLEPGVYGAHSADEFWFDRKEGFCEHIASAFVVLMRAMDIPARIVTGY
                     QGGERNPVDGYWIVRQSDAHAWTEVWLSGRGWVRVDPTSAVAPGRTAAFQRLQAPRGA
                     FATALNNVTPDFAAGLRSAWDALNNGWNQWVLNYTQSKQLELLKDLGFPSPSWVDLSF
                     VLLALLVVVAMCVAAWTLWERREHDPWLRLLARVRSRLAEAGMELPPAAPPRQIATLV
                     TGRFGNSAQALADWLLKLESQRYARTPGAGLGALQREFNHLAWPR"
     gene            7622..8599
                     /gene="mltB"
                     /locus_tag="VGA59_08220"
     CDS             7622..8599
                     /gene="mltB"
                     /locus_tag="VGA59_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEX9719702.1"
                     /translation="MPYAGNEEALRWADEAAQRRGLDRDWVRRAVERAQFLPVVARLM
                     QPAPAGTPKNWRVYRSRFIDAVRIDAGLRFWQDNQDVLERAEKQYGVPAEIIVGIVGV
                     ETIYGQQTGNFRVIDALATLAFNFPESHPRAAERRDYFRGELEHFLVLQSQRQADPLG
                     PLGSYAGAMGMAQFMPSSWLRWAVDFDGDDRIDLTRSPADVIGSVANYFKSYGWQPGM
                     PTHYPVSFDPATLDKDALLAPDILPTFSVASFMAKGIVLWGPALEHKGPLALVELQNG
                     DDEPQYFAGTENFYVITRYNWSSYYAMAVIELAREVKEAWAGAAAAARP"
BASE COUNT         1398 a         2950 c         2891 g         1441 t
ORIGIN      
        1 gcgatctcgt caaaggggtt catgctcatc ttgacgttgg cgatgtccac ccccgtgttg
       61 tccgacttga ctcggacctt cacgttgaag tcgaccaccc gtttgaccgg taccaggacc
      121 ttcatgctcg cggctcctaa aagtttggga attgacgtaa acgtaaactt gaattctatg
      181 cggcgctgca ccacgccgtc ttcaccgggg cgacagtaca aaaaagaacg accgttcgtt
      241 tttggattat aggcggctgg ttttcgcgcg gtctagggtc ggccgccaaa aagtgtgtca
      301 ggcctccgac aattcgccgt tccactgatc cgaaatccgg ggacgcgccg gcctggcacc
      361 ctgcagttcg cccgaggcgc agctcagcct gtgcttgccg tcgccgatga agcggtcttc
      421 aggtgcacaa cccgctgcgc gtacgggatg tcgatgccgg cttcgcgcag gcccttgagc
      481 accctcaggt tgacgtcgga cttgacgttg agctggccgt tggaggggtc ggcgatccag
      541 aacagcagcg tgaactccag cccgtcggcg ccgaagcgcg ccaggcgcgc gccgggcggc
      601 ggatcggcga tcacgcggtc ggtgccgacg gcggcgccca ccaggatctg ctgcacccga
      661 tcgacgtcgc tgtcgtatcc gaccgtcacg tccgtggtca gcaggatacg cggatcggcc
      721 agcgagaggt tctcgatgcg ttcggtgatc atcttttcgt tgggaacgat cgactcgcgg
      781 ccgctcaggg agcgaatcag cgtatagcgc gtcttgatgt ccatcaccgt gccttcgaac
      841 tggtcgacgc gaaccgtatc gccgatgcgc agactgcgct cgaacaggat cacgaaaccg
      901 ctgacgtagt tggccgcgag cttctgcagg ccgaagccca ggcccacgcc gatcgcgccg
      961 ccgagcaccg acagcgccgt gaggtcgacg ccggttgccg acagcgcgaa cagcaggccc
     1021 accagcagca gcagcgcccg gatcgcgttg gccgcgacct tgcgcagcga gaggtcgtcg
     1081 acggtctcgc gcaggatgcg cttttccagc gcggccgaaa tccacagcgc cagcaccagt
     1141 acgacacccg aagacaggag cccctgcacc aggctgagca ggctcacctt ggacttgccg
     1201 aagacgaagt ggatggcttc catctcgtcc atcacggcgg gcagcaaacc cacgatccac
     1261 agcacggcgg ccatccaggc cagccaggag aacagccgtt ccacgaggcg cgcgaaccgc
     1321 gatgccggaa agaccaccgt caatacgcgc gccaggaacc ggatgccggc cagcgagatc
     1381 agcaccggca ccgccaccct gagcaatgcc accggttgcc gctgggccag caccagcatg
     1441 gcgctgtacg tcagcaccaa ggcaacgagg ggaaagagca ggccgtccac ggtggcgcgg
     1501 ccgaaccaga ctgaatcgct cgaatgcctg cgcccgacca gccagcacac gccgaacgcc
     1561 agcgccaggc agcccagcag tacgccgagt tcccacggca ctcccggatg gcccaggtcg
     1621 cggaccagtt tgtcgagttc agtcattcag agagacgagc ttcagctctt ccaggccggc
     1681 ttgcgtttct ccgtgaaagc cgtgacgccc tcctgcgcgc agccttccat catgttcacg
     1741 gccatcgtct gcgaagcgag ctggtacgcc gcctcgatgc ccatctcgcg ctgccggtag
     1801 aacagctcct taccgatggc gatcgcggtg cggggcttgc ccaggatgct cgccaccagc
     1861 ttctcgactt ccgcatcgag ctgctccggc gggcagacgc ggttgaccag gccccgttcg
     1921 agcgcctgct gcgccgtgat gaactcgccc gtcaccagca tctccattgc ctgcttgggc
     1981 gcgatgttgc gcagcagggg cacactgggc gtggaacaga agaggccgaa gttgaccccg
     2041 ctgacggcga agctggccgc gctcgacgcc accgccaggt cgcactgcgc caccagctgg
     2101 cagcccgcgg cggtggctat gccctgcacc ttggcgatca ccggcaccgg cagtttctgg
     2161 atgccgagca tcatgcggct gcattgcgcg aacaggcgct ggtaatactc cagcgagggc
     2221 ttcgcgatca tctctttcag gttgtggccg ggacagaacg ccttgccggc ggcggcgagc
     2281 acgaccacgc gcgccgaagc gtccttcgcc acccgatcca gcgccgcctg cagcgcatcg
     2341 atcatctcct cgccgagcgc gttgaaagcg cccgcgcggt tcatcgtgag cgtgaccacg
     2401 ccgcgctcgt ctttcttctc gatcagtgct tcatccatgt gttcgctccg ggtccagcaa
     2461 gtattgtcag atatccgcca gcacgcgcaa atgcgcctcc acgctgcgcc cgagcgcgct
     2521 caggttgtag ccgccttcca gcgaagagac gatgcgcccg gacgcgtact tcttcgcgat
     2581 ttccttgacc ttcatggtga tccaggtgta gtccgcctcc acgagaccca attgcccgag
     2641 gtcgtcctcg cggtgcgcgt cgaagccggc gctgatgaag atcatctcgg gtctgaagtc
     2701 tgcgaggcgc ggcatccagg cctgctcgac cagctcacgg atggccatgc cccgggtgta
     2761 cgcgggcacc ggcaggttca ccatgttcgg gtgatggccc tcggtgccgg tgtaggggta
     2821 gaacggatgc tggaagaatc ccaccatgag agcgcgctcg tccccgccca ggatgtcctc
     2881 ggtcccgttg ccatggtgca catcgaaatc cacgatcgcc acgcgcttga ggccgtggcg
     2941 ttccagcgcg tacttcgccg cgatggcgat gttgttgaag aaacagaagc ccatcgcgcg
     3001 gtcccggcag gcatggtggc cgggcggccg caccgcgcag aaggcgtttt ccagctcgcc
     3061 ggcgatcacg gcgtcggtag ccgacagggc ggcgccggcg gcacgcagcg ccgctttcca
     3121 ggtgtccgga ttcatcagcg tatcggggtc gatctgcgcg tagccgcggc cgccggcggc
     3181 aatatgttcg tccagctcct ggtccaggcc gcgcagggcg gccacatgca tgcgcgtgtg
     3241 cgccagttcg atgtcggccg gcgaagcctc gtgtgcctgg cgccgttcca gcgcaaggcc
     3301 gacgccgctg gcgatcagcc ggtcctcgat tgcatcgagc cgttcgggac actcgggatg
     3361 gcccggcccc atttcatgtc tcctgcaatc cggatgcgtg taatacccgg ttttattcat
     3421 agctggcgtc cgacttccgc gaattgggat aacgtcatgc ccatgatgga tgcacaacaa
     3481 aaactccaag cgctcctgga ccagcttaac acggtgatcg tcggaaaacc cgtccaggtg
     3541 cgcgactgtg tggcgtgcct gctggcgggg ggacacctgc tgatcgagga tgtgccgggc
     3601 gtgggcaaga ccaccctggc ccatgcgctg tcgcgggcgt tgggcctgca cttttcgcgg
     3661 gtccagttca cggccgacct gatgcccagc gatctctcgg gtgtgtcgat ctacgagcgg
     3721 ggcaaggagg ccttcgtctt ccatcccgga cccgtgttcg cgcaggtcct gctggccgac
     3781 gagatcaacc gggccagccc caagacccag agcgccctgc tggaggcgat ggaggaaaag
     3841 caggtcaccg tggaaggcga aacccgggcc ctgcccacgc cgtttttcgt gatcgccacg
     3901 cagaacccgc acgaccagct cggcaccttt gccctgccag agtcgcagct ggaccgtttc
     3961 ctgatgcgca tctcgctcgg ctacccggac cgtgccgccg agcgcgagct gctcaacggg
     4021 gccgaccggc gcggcatggt ggacgccatg cgcagcacgt tcacgccggt ggacctgctg
     4081 cagctgcagc agaaagtgct ccaggtccac gcggccgggc ctttgctcac ctatgtgcag
     4141 gacctggtgg cggcgacccg ctcgggccgc tggttcctgc agggcctgtc accccgcgcg
     4201 gggatcgcgg tggtgagggc ggccaaggcc caggccctgc tcagcgggcg ggactacgtc
     4261 gcgccggacg atgtgcaagc gatcctgccc cagacggttg cgcatcgcct gatcccggtg
     4321 ggcgatgccg gccgcggccc gatcgagcag gtgcgggcca tgctggaagc ggtgccgctg
     4381 ccgtgaaaga ccacccccga agcgccttcg gcgatgcccc ctcgggtttc gccctctccc
     4441 cctcgagggg gcgccaccag cggcccggca aagccggttc cgcggtggcc ccccgcactg
     4501 cagggccgga acgacaacgc atgaacgcgg gcgttcttga tcccgtcacg ttcgtccgcc
     4561 ggcagttccg gcgctggtgg caggcgcgcc tgccgctcgc ggacacgatc gagctgaccc
     4621 agcgcaatgt ctacatcctg ccgacccggc cgggcgtgat gctgggcctc acgctggtcg
     4681 tgctgctggt gggctccatc aactaccagc tgaacctggg ctacctgctc actttcctgc
     4741 tcgccggcag cgctgtcgtg ggcatgtacg tgtgccacgg aacgctgcgc gggctgacga
     4801 tgaacctggt gccgccggac ccgcagttcg tgggcgccag cgcgaccttg tcgatcgtgc
     4861 tgacgaatga ccgcagatcg acccgatttg gcatcggact tgccgtgctg gaagcaacgc
     4921 acgaagaccg ctgggtctgg accgacgtgc ctcccctagg gcaaagcacg gtgcaggtgg
     4981 cgttcaagcc ggagcgccgc gggctgcacc gggtgccacc gctcaccgcc gaaacgcgct
     5041 tccccctggg caccttccgg gtgtggaacg tctggcggcc ggccgcgcag gtgctggcct
     5101 atcccgcgcc cgagactttt ccaccgccgc tgccgccggg tgagccgcgc gcgggcggcg
     5161 ctgccagcgc gagggttcag agtaccggcg aattcgacgg cgtgcgggcc taccggcgcg
     5221 gtgatccgct gaagctggtg gtctggaaga aggccgcgaa ggccgacgag ctggtcagtc
     5281 gcgacaacga gcaagcccaa cgctacgagc tgtggctgga ctacgcgcaa gcgggcggcc
     5341 tgggcgccga gcacacgctg tcacggctca cggcgtgggt gcttcaggcc gagcgcctcg
     5401 gcctggacta cgggctgcgc ctgcccggcc aggagatagc ggccgcgacg ggcgaagccc
     5461 acaagcggcg ctgcctggag gcgctcgcca catgttgagc cggttgaacg ccctgccgcg
     5521 cgacagccgg gacaccttgt tcctgctgct ggtgatcgga tgggtgatcc tgccgcaagt
     5581 cgcgaagctg ccgctgtggt gcagcgcgct ggccggcgcg gtgctggtgt ggcggggctg
     5641 gctcgcggtg gcctcccggc cgctgccggg caaatggtgg ctgctcggcc tgctcgccgt
     5701 cacggtcgcc gcgaccttcg ccacccaccg cactttgctg ggccgcgacg cgggagtgac
     5761 gctgatcgtc gtgctgctgg cgctcaagac gctcgagctg cgcgcgcgcc gcgacgcgtt
     5821 cgccattttt ttcctgggct tcttcacgat gctcacgaac ttcttcttct cgcaatcgct
     5881 gccgacggcg gcggccatgc tggtggccct gctgggcctg ctcacggcac tggtcaatgc
     5941 ccatatgccg gtgggcaggc cgccgctggc gcaggcggcg cgcactgcgg gctggatggc
     6001 gctgctgggc gcgccgatca tggccgtgct gttcgtgctg ttccccaggc tggcgccgct
     6061 gtggggcatt cccagcgacg cgatgagcgg ccgcagcggc ctgtcggcca gcatgcaggt
     6121 gggcagcatg gccagcctgg ccctggacga aaccatcgcg atgcgggtga agttcgatgg
     6181 accgccgccg agccaaggcg acctgtattt ccgcgggccg gtgctctccg cgttcgacgg
     6241 ccgcgagtgg cgcccgctgc aatcgcgcct ggggtcgccc ttctcgcctg ttgttccggg
     6301 cacggcgcaa ctcgacgtgc tgggcgagcc ggtgcgctac gaggtcacgc tcgagccgaa
     6361 caaccgtcca tgggtgctgg tgctggacgc cgcgatgaag acgccgaccg tcccgggcat
     6421 gcgggccgtg atgaccagcg aactgcagtg ggtggccaat cggccggtga cggacttgct
     6481 gcgctatggc gccgaaagcc atctggtctt tcggcatggg ccgcgcacca gcgcggcggc
     6541 cgtcccgccg caatacatcg cgctgccacc cgggttcaat ccgcgcacgc tcgcactggc
     6601 ggacgagttg cgccgcgaac cgcgactggc cgccggcggg cctgcggccc tggtccaggc
     6661 cgcactcgac cggctgcgca cgggcaatta ccaatacacg ctggagcccg gcgtttatgg
     6721 cgcccattcc gccgatgagt tctggttcga ccggaaagaa ggcttttgcg aacacatcgc
     6781 gtcggctttc gtcgtgctga tgcgagccat ggacattccg gcccgaatcg tcacagggta
     6841 ccagggcggc gagcgcaatc cggtggacgg ctactggatc gtgcgccaga gcgacgccca
     6901 cgcctggacc gaggtgtggc tgtccggccg gggctgggtg cgcgtggatc ccacttccgc
     6961 ggtggctccc gggcgcaccg ccgccttcca gcgcctgcag gcgccgcgcg gcgctttcgc
     7021 cacggcactg aacaacgtga cgccggactt cgcggcgggc ctgcgctccg cgtgggacgc
     7081 gctgaacaac ggctggaacc aatgggtcct gaactacacg cagagcaaac agctcgagct
     7141 gctgaaggac ctcggcttcc cgtcccccag ttgggtggac ctgagttttg tactgctggc
     7201 gctgctcgtc gtcgtggcga tgtgcgtcgc cgcctggaca ctgtgggaac ggcgggagca
     7261 cgatccatgg ctgaggctgc tggcccgcgt gcgcagccgc ctggccgagg ccggcatgga
     7321 attgccgccg gcggcgccgc cgcgtcagat tgcgaccctt gtcacagggc gtttcgggaa
     7381 tagcgcccag gcactggcag actggttgct gaagctggaa tcgcagcgct acgcccgcac
     7441 cccgggcgcc ggcctcggtg cgctccagcg cgaattcaac cacttggcct ggccccgctg
     7501 aatgccacga ttcctgctca tcaccacact tttcctagcg gcaagcttgc tttacgcgcc
     7561 ggcatcgtcc cagccagccg ccaagaaacg cagcgcggcc gagcgcaaca gccagcgggg
     7621 catgccctat gccggcaatg aggaagccct gcgctgggcc gacgaagcgg cgcagcgccg
     7681 cggactggat cgcgactggg tccggcgtgc ggtcgagcgc gcgcagttcc tgccggtcgt
     7741 tgcccgtttg atgcagcccg ccccggcggg caccccgaag aattggcgcg tctatcgcag
     7801 ccgcttcatc gacgccgtcc gcatcgatgc cggcttgagg ttctggcagg acaaccagga
     7861 cgtgctggag cgcgcggaaa agcaatacgg tgtgccggcc gagatcattg tggggatcgt
     7921 cggcgtggag accatttacg ggcagcagac gggaaacttc cgcgtgatcg acgcgctggc
     7981 cacgctcgcc ttcaactttc ccgagtcgca cccgcgcgcg gccgagcgcc gcgactactt
     8041 tcgaggcgaa ctcgaacact tcctggtgct gcagagtcaa cgccaggccg atccgctcgg
     8101 gccgctgggc agctacgcgg gcgccatggg catggcgcag ttcatgccca gcagctggtt
     8161 gcgctgggcc gtggattttg acggcgacga ccgcatcgac ctcacccgca gcccggccga
     8221 tgtcatcggc tcggtggcca actatttcaa gagctacggc tggcagccgg gaatgcccac
     8281 ccattacccg gtgagcttcg accccgccac gctcgacaag gacgccctgc tggcgcccga
     8341 catcctgccg acattcagcg tggccagctt catggccaag ggcatcgtgc tctgggggcc
     8401 cgcgctggag cacaagggac cgctggcgct ggtcgagctg cagaacggcg acgacgagcc
     8461 gcaatacttc gccggcaccg agaacttcta cgtgatcacg cgctacaact ggtcgagcta
     8521 ttacgcgatg gccgtgatcg agctcgcgcg cgaagtcaag gaagcatggg cgggggccgc
     8581 ggcagccgcg cggccctgac gcttcagaag gacgatttca cgcgcggccg gcggtgtgaa
     8641 actcaggccg gcacggcaat caggaagtca cggctgatgc
//
LOCUS       DASWIY010000225        19068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_516695, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000225 DASWIY010000000
VERSION     DASWIY010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 19068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19068
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516695"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..451
                     /locus_tag="VGA59_08225"
     CDS             2..451
                     /locus_tag="VGA59_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX9719703.1"
                     /translation="MKPAICALGLLALAAALPPAAAQDAQLNLPRVTLSAGMHRIDAQ
                     VAHTPGQRSIGLMYRKEMPQHEGMLFAFEQPSEQCFWMKNTLLPLTAAFVADDGTIVN
                     LADMKPQTTESHCSDKPVRYVLEMNQGWFAKKGVKPGAKLAGSPFRK"
     gene            complement(464..1042)
                     /locus_tag="VGA59_08230"
     CDS             complement(464..1042)
                     /locus_tag="VGA59_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX9719704.1"
                     /translation="MEHVLPPLPYPIDSLAPHYSRETLEYHHGKHHNAYVTNLNNLQK
                     GTEFENMTLEEIIKKSSGGIYNNSAQVWNHTFFWNCMKPGGGGEPAGPLAAAINSKWG
                     GYAAFRDAFVKSAVGNFGSGWTWLVKKPDGSVDIVNTGAAGTPLTTADRALLTVDVWE
                     HAYYIDYRNLRPKYVETFFDKLVNWSFAEKNW"
     gene            complement(1090..2376)
                     /gene="xseA"
                     /locus_tag="VGA59_08235"
     CDS             complement(1090..2376)
                     /gene="xseA"
                     /locus_tag="VGA59_08235"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX9719705.1"
                     /translation="MAEPYHPPLAPRIWQVGALCRAIADALEARFNPVAVRGEISGFS
                     RAASGHCYFSLKDAQGQIRCAMFRRAAGLMDFLPGDGEMVEVLGRLDIYEPRGDLQLI
                     VESLTRAGPGALFEQFLQLKARLDAQGLFDPARKRPVPVLPKGIGVVTSPGAAALHDV
                     VTALRRRAPHVPVALAPAAVQGSQAPAELIRALRALYRLGETGSVDVILLVRGGGSIE
                     DLWAFNDEQLARTIVQSPVPVICGVGHETDFTIADFCADVRAPTPTAAAELAAEPRDS
                     WLQGLERFEARLQGAVRGRLDSAGQRLDLAASRLGRPLARMGGQQLRLGRAAQRLRYA
                     AMARLERDKGRIQATQARLKHAVQARLQRAAQVFDRSRLRLELLDPRLVLERGYALLT
                     NEAGQPVSSVKQTRPGQSLRATLADGEVPLTVSPSP"
     gene            2708..3340
                     /locus_tag="VGA59_08240"
     CDS             2708..3340
                     /locus_tag="VGA59_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9719706.1"
                     /translation="MFSIIQAAGWPIWPLVACSIIALALVIERFISLKTAKVAPPRLL
                     DEALSVSRTSVPSSDVVRQLEQNSALGEVLASGFRALNSDPRCSEVDLRAHMEHTGRE
                     VAHRLERYLSALATIASAAPLLGLLGTVIGMIEIFGSQTPSGGAPGGNPAQLAHGISV
                     ALYNTAFGLIVAIPSLIFWRYFRVRVDEYLLTLELSAERFARHLNALRVR"
     gene            3337..3765
                     /locus_tag="VGA59_08245"
     CDS             3337..3765
                     /locus_tag="VGA59_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9719707.1"
                     /translation="MNFRPRQKEEPEINLIPFIDVLLVVLIFLMLTTTYSKFTEMQVK
                     LPVADADAQRDYPNEVIVGVSSDGRYSIKGVPVAGRSVEAVARALGEAASAGKDSVII
                     ISADAAAPHQSVITVMEAARRSGLVQITFATQSSARAGSR"
     gene            3769..4758
                     /gene="lpxK"
                     /locus_tag="VGA59_08250"
     CDS             3769..4758
                     /gene="lpxK"
                     /locus_tag="VGA59_08250"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX9719708.1"
                     /translation="MAQALQRAWLRRGALARVLWPLSLAFGALAALRRAAYRMRLLES
                     ERLAVPVVIVGNVVAGGSGKTPVVMAVVDHLRSRGLAAGVVSRGYGRSTSDCREVRPD
                     SRAVEVGDEPLLVAQRCEVPVFVAASRAEAARALLAAHPATQVIVSDDGLQHYRLQRD
                     IEICVFDERGTGNGWLLPAGPLREPWPRSVDLVLSPGAATGIAGFEARRRLAAVAVRG
                     DGTRIELASLKGRPLKAVAGIARPEAFFAMLRAQGLQVVQAVGLPDHHRFNEPVSAEY
                     ELICTEKDAVKLWRLRPEAWAVPLRLEVEPQFWAALDRLLDAKLSSRHGSQTS"
     gene            4739..4945
                     /locus_tag="VGA59_08255"
     CDS             4739..4945
                     /locus_tag="VGA59_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX9719709.1"
                     /translation="MDPKLLELLVCPVTKGHLDYDRTRQELLSRSARLAYPIRDGIPI
                     LLEEEARTLSDEELERLPARTPLV"
     gene            4990..5748
                     /gene="kdsB"
                     /locus_tag="VGA59_08260"
     CDS             4990..5748
                     /gene="kdsB"
                     /locus_tag="VGA59_08260"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX9719710.1"
                     /translation="MGFCVLIPARLASTRLPDKPLADIGGQPMVVRVAQRASASSARR
                     VVVAADSASIVDACRTHGVEAILTRGVHASGSDRVAEACELLGLSGRDLVVNVQGDEP
                     LIEPALIDSVAALLETHPEADMSTAAHPIDSVGDFTNPNVVKVVLDVRGFALYFSRAP
                     LPWWRDGFASGIHALPQPRPLRHVGIYGYRADFLRGFPRLAPAPLEVAEALEQLRALW
                     HGHRIAVHVSATAPGTGVDTPEDLERVRALWREA"
     gene            5853..6509
                     /gene="adk"
                     /locus_tag="VGA59_08265"
     CDS             5853..6509
                     /gene="adk"
                     /locus_tag="VGA59_08265"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX9719711.1"
                     /translation="MRLILLGAPGAGKGTQAAFICQKYGIPQISTGDMLRAAVKAGTP
                     LGLAARKVMDSGALVSDEIIIGLVKERIAQPDCANGFLFDGFPRTLPQADAMRAAGVK
                     LDYVLEIDVPFDAIVERMSGRRSHPASGRTYHIKFNPPKVQGKDDVTGEPLIQREDDK
                     EETVKKRLEVYAAQTRPLVEYYSEWAKADPVNAPKYRAINGTGTVEEIKARALDALSS
                     "
     gene            complement(6668..7609)
                     /locus_tag="VGA59_08270"
     CDS             complement(6668..7609)
                     /locus_tag="VGA59_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX9719712.1"
                     /translation="MQVNGKKIAVLATGGTIAGRAEAAHDNIGYTAAQMGVQELLAAI
                     PALASLPLIAEQVAQIDSKDMSYEIWRTLALRCAHWLAQDDVGGVVITHGTDTLEETA
                     YFLHAVLAPAKPVVLTCAMRPATALVPDGPQNLLDAIAVARAAGAHGVVAVCAGTVHG
                     APDVAKQHTYRLDAFTSGDAGPIGYVEEGRLRRLRDWPAADPGAARAFGNFTAGQRWP
                     RVEIIMNHAGAGAAIVQALVDQGVDGLVVAGTGNGSVRRELEEALRQAQARGVKVVRS
                     TRCPQGPVLARPDDTLPGAGHLSPVKARIALMLELLG"
     gene            7669..8343
                     /gene="lexA"
                     /locus_tag="VGA59_08275"
     CDS             7669..8343
                     /gene="lexA"
                     /locus_tag="VGA59_08275"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX9719713.1"
                     /translation="MLDTPKLTARQQQILDLIQSAIARTGAPPTRAEIASELGFKSAN
                     AAEEHLQALARKGVIELVSGTSRGIRLKSDALRAINESRNKQFSLPLQTLAQLALPLI
                     GRVAAGSPILAQEHVDQTYYVESSLFQRRPDYLLKVRGMSMRDAGIMDGDLLAVQATK
                     DAKNGQIIVARLGDDVTVKRFRRNKHLIELHAENPDYPTIIVEPGEPFEIEGLAVGLI
                     RNTMLM"
     gene            8459..8785
                     /locus_tag="VGA59_08280"
     CDS             8459..8785
                     /locus_tag="VGA59_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719714.1"
                     /translation="MGIAMLTFGGLLAPLRSLAGWLAHAPNARQGTDQGHLPTASFAA
                     SRACPARPVPPGSSRERPRRPLRVVRVVEASRVPAAAGRMIISGRMADVCAELDRLAA
                     LEATAR"
     gene            complement(8815..9330)
                     /locus_tag="VGA59_08285"
     CDS             complement(8815..9330)
                     /locus_tag="VGA59_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX9719715.1"
                     /translation="MTISMSSASLPVFKTMLGNLSHILDKAQAHAEARKFDPAALLQF
                     RMAPDMLPFTRQIQIACDAAKNGMARISGVEAPKFEDVETTFPELKARIRKTLDYLGT
                     VPAERLDGTEDKDITFPVGRETTRTMKAEAYLKHWALPNFFFHITMAYAILRHNGVEL
                     GKSDYLVGGGA"
     gene            9430..10437
                     /locus_tag="VGA59_08290"
     CDS             9430..10437
                     /locus_tag="VGA59_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX9719716.1"
                     /translation="MNIVILDDYQDAVRKLRCAAKLEAYPAKVYTNTVKGIGQLSVRL
                     RDVDVIVLIRERTHITRQLLEKLPRLRLIAQTGRVGAHIDLQACTERGIAVAEGVGSP
                     VAPAELTWALIMAAMRRLPQYISTLKHGGWQQSGMKSASMPPNFGLGVVLRGRTLGIW
                     GYGKVGQLVAGYGQAFGMKVIVWGSQDSRQKAVSDGVQAAASREELFTQSDVLSLHVR
                     LSDESAGIVTLDDLSRMKPTSLLVNTSRAELVEHDALIAALNRGRPGMAAVDVFESEP
                     ILQGHALLRLENCICTPHIGYVEIDSYEMYFGAAFDNVVNFIKGTPTNIVNPGALQVR
                     R"
     gene            10521..11705
                     /locus_tag="VGA59_08295"
     CDS             10521..11705
                     /locus_tag="VGA59_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9719717.1"
                     /translation="MNPPAPAAAKPALPAVLWPLLFGNFVIGTGVMVVPGALNEISDS
                     LQVSVATAGQLISAGALLMCIGAPLFAALVAGWDRRRLLALSMVWYGALHLASIAAPT
                     FAALLPLRVLTMVSPAIFTPQAAACVGLLVLPEQRGRAITFVFLGWSLASVLGLPIGA
                     YVGGTLGWRSAFGLVGLLSLGSALWVWRTMPDGVKPPALSAAAWREAFRSRALMLCVV
                     VTVLYSAAQFVLFSYFAPYYKLKIGITPGELSLLLMWFGVFGFLGNMVMSRHIDRIGA
                     SRAVMAGIAAMALSMLIWPLGTSLALAALVAVPWALGCFASNSAQQARLVGIAPALSA
                     GSIALNTSAMYAGQAIGAAGGGWLIARDGMDLLHWAGFAGLLLSMALSALASRYRHVP
                     RH"
     gene            11739..13259
                     /locus_tag="VGA59_08300"
     CDS             11739..13259
                     /locus_tag="VGA59_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX9719718.1"
                     /translation="MTLLFDTVGVVGVGAMGRGIAQIAAQAGSIVKLYDTQPQSTAKA
                     RDELFSQWDRLLEKGRMDGAAVQACKQRLQVAEGLQALADCNLVVEAVVERLDVKKSL
                     FAELEAIVASTAVLTTNTSSLSVTAIAAGLKRPHQFAGYHFFNPVPLMKVVEVVAGLK
                     TDPAVCKRLAEYAGQMGHTAVQAQDTPGFIVNHAGRGYGTEALRLVGEGVADFATVDR
                     ILKDQAGFKLGPFELMDLTALDVSHPVMESIYRQYYDEPRYRPSVITAQRLAGGVVGK
                     KVGEGFYRYVDGKAQVDAEPAAPQVAEMPPVWVSTRAARRSELYQLLKDLGAKIETGQ
                     SPSPQALSLVAPLGFDVTTVAVVERLDPARTLGIDMLIDDAATKRRVLATNPATRADM
                     RDAAHALFARDGKAVSVIRDSGGFVTQRVVAMIVNIAADICQQRICSPRDLETAVTLG
                     LGYPLGPLAMGDRWGPTNVLEVLFNMQTVYGDPRYRPSPWLRRRGAIGLSLLHEET"
     gene            13262..14041
                     /locus_tag="VGA59_08305"
     CDS             13262..14041
                     /locus_tag="VGA59_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9719719.1"
                     /translation="MAAELKSTSQGRTMVLTLSNPEHRNALGPEMYAAGVEALSVAES
                     NPEVRSVVIAGEGKVFSAGGNLQRLQANRQLPPQVQAQSVESLHSWVEAIRTFPKPVI
                     AAVEGAAAGAGFSLALACDLIVAADDAIFVMAYINVALSPDGGASWSLSQALPRQLVG
                     ELLLGGERMTARRLHELGVVNRVTPGGGALDEAMALAEKLNAKAPNAVASMKELINDA
                     AGTTLNQQLAAERDHLVRNLHHANAGIGIAAFLARQTPDYQ"
     gene            14136..14855
                     /locus_tag="VGA59_08310"
     CDS             14136..14855
                     /locus_tag="VGA59_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9719720.1"
                     /translation="MDDPILTIDEREAINAGRWFSSLSPSLRHDILRCAYVKRYKDGE
                     LISARGDPSEEWIACAKGAVRVSSTSISGKQVTLTYVEPGIWFGDVSIFDGERRTHDA
                     YAHGDTTILCVARGDLRKILSQHVELYEALLRLHARRIRQLFGLVEDLNTLPLRARLA
                     KQLTHLVRSYGVPSLSDGREVRISLQLAQEELAQLLGASRQRVNQELKAMERDDVIRI
                     EPGGLVVRNREALMRIIEADN"
     gene            14950..16053
                     /locus_tag="VGA59_08315"
     CDS             14950..16053
                     /locus_tag="VGA59_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX9719721.1"
                     /translation="MSDFDHFVGTRPVSGKHVFDTAALTAWLEKNLEGFAGPIGVEMF
                     KGGQSNPTYKLVTPGQSYVMRAKPGPVAMLLPSAHAVEREFAVMRGLAGTDVPVPRMY
                     CLCEDESVIGRAFYIMEFMQGRVLWDQALPEMSPRGRGEIYDEMNRVISALHTVKFAE
                     RGLANYGKPGNYFDRQIGRWSKQYKASADGAGAMSQPIPEMERLVEWLPAHIPAAGRD
                     DSKVSIVHGDYRLDNLMFHPGQPRVIAVLDWELSTLGHPLADFSYHCMAWNIPHGSFR
                     GIGGLDHAALGIPSQEEYIHRYCERTGLADAGDLRADWNFYMAYNMFRIAAILQGIAK
                     RVEAGTASSAQAASSGASARPMAELAWKFAQQA"
     gene            16088..17353
                     /locus_tag="VGA59_08320"
     CDS             16088..17353
                     /locus_tag="VGA59_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9719722.1"
                     /translation="MDFEYSPKTRELQTKLLKFMDDYIYPAEVQYQQELEANTKAGKR
                     WSALQTIEKLKPKARAAGLWNLFLPVDSSSASGYEGAGLTNQEYAPLAEIMGRVMWCS
                     EVFNCSAPDTGNMETIARYGSEEIKARWLKPLLEGEIRSAFAMTEPEVASSDATNICT
                     RIERHGDEYVINGHKWWISGAADPRCAVFITMGKTDPQAPRHSQQSMVIVPADTKGIR
                     IERPLTVYGYDDAPHGHVEMYFEDVRVPVSNILLGEGRGFEIAQGRLGPGRIHHCMRM
                     IGLAERSLELMCKRASSRVAFGKTVAQQSVTQERIAEARCRIDMARLLTLKAAWLMDV
                     AGNKAAKTEIAMIKVVAPTMALQVIDWAIQVHGGGGVSGDFPLAHSWAAARTLRFADG
                     PDEVHRMAVAKWELGKYGSPSREAAASRG"
     gene            complement(17342..18061)
                     /locus_tag="VGA59_08325"
     CDS             complement(17342..18061)
                     /locus_tag="VGA59_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719723.1"
                     /translation="MFDRIRKAFSSDAKEPVEAAASTSDPVHGPVSEWAATQGFGFSV
                     DRTGHGVSLEGKVSGKPWRLQLGSPSRQYIRGDEVRARAELGIDEDVAIVVMSRPLKE
                     ALEKQAYQIYTDNLQTSIDSNLPEEMRWLAMYEEVGWESLPAEFWERYAVMSDQRESA
                     LAWVDPALARLMLDWPEPAPSAEVPFVMLLLRGKAYLRMEYSPADLATLRHAALIFTS
                     ACESALGSLAGPIAPDLSLPA"
     gene            18119..18811
                     /locus_tag="VGA59_08330"
     CDS             18119..18811
                     /locus_tag="VGA59_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX9719724.1"
                     /translation="MIDVFSWPTPNGHKVHIMLEECGLAYRAIPVDIGAGDQFKPEFL
                     KISPNNKIPAITDPDGLDGKPISLFESGAILVYLAAKTGLFLPRTDRAKYEVLQWLMF
                     QMGSVGPMLGQNHHFRLYAPEKLEYAVQRYTNEAKRIYGVIDQRLSQNEYLGGSQYSI
                     ADIATFPWLRSWERQGIVLGEYPYLQDWFNKMAERPAVQRGVRVMAELRRPITGEKER
                     EILFGKTQYERH"
BASE COUNT         3267 a         6376 c         6223 g         3202 t
ORIGIN      
        1 catgaaacca gctatctgcg cccttggctt gctggcgctg gcggcggcgc tgcctccggc
       61 cgccgcccag gatgcccagt tgaacctgcc ccgcgtgacc ctgtccgccg ggatgcaccg
      121 gatcgatgcc caggtcgccc atacccccgg ccaaagatcc atcggattga tgtatcgcaa
      181 ggaaatgcca cagcacgagg ggatgctgtt cgccttcgag cagccgtccg aacaatgttt
      241 ttggatgaaa aacacgctgc tgccgctgac agccgcattc gtggccgacg acggcaccat
      301 cgtcaacctg gccgacatga agccgcagac cactgaatcg cactgctcgg acaagccggt
      361 gcgctatgtg ctggaaatga accagggctg gttcgcgaag aaaggcgtca agcccggcgc
      421 gaagctggcc ggctcgcctt tcagaaaata agttccaagg cccctaccaa ttcttctcgg
      481 cgaagctcca gttcaccagc ttgtcgaaga acgtctcgac gtacttcggg cgcaggttgc
      541 ggtagtcgat gtagtaggcg tgctcccaga cgtccacggt cagcaaggcc ctgtcggccg
      601 tggtcagcgg cgtgccggcc gcgccggtgt tcacaatgtc cacgctgccg tcaggcttct
      661 tgaccagcca ggtccagccc gaaccgaagt tgcccacggc ggacttgacg aatgcgtcgc
      721 ggaacgccgc atagccgccc cacttgctgt tgatggcggc tgccagcggg ccggcaggct
      781 cgccaccgcc gcccggcttc atgcagttcc agaaaaaggt gtggttccag acctgggccg
      841 agttgttgta gatgccgccg gaggatttct tgatgatctc ctcgagcgtc atgttctcga
      901 attcggtgcc tttttgcaga ttgttgaggt tggtcacata ggcgttgtga tgcttgccgt
      961 ggtgatactc cagcgtctcc ctcgaatagt gcggcgccag cgaatcgatc gggtacggga
     1021 gcgggggcag aacgtgttcc atggaattcc tcttgttgtc tggtgagggg gctgattcta
     1081 agaactaaat caaggcgatg gtgatacggt cagtggaacc tctccatcgg cgagggttgc
     1141 tcgcaaggac tgcccgggtc gggtctgctt cacgctgctg accggctgcc cggcctcatt
     1201 ggtcagaagc gcatagccgc gctccaggac gaggcgcgga tccagcagtt ccagtcgcag
     1261 gcgcgagcgg tcgaacacct gcgccgcgcg ttgcagccgc gcttgcaccg catgcttgag
     1321 ccgggcttgg gtcgcctgga tacggccttt gtcccgctcc agccgggcca tggccgcgta
     1381 gcgcaaacgt tgagcggcgc ggcccaggcg cagctgctgc ccccccatgc gagccagcgg
     1441 acgccccagt cgcgaggctg caaggtccag tcgctggccg gcgctgtcca atcggccccg
     1501 caccgcccct tgcaatcgcg cctcgaaccg ctcgagtccc tgcagccagc tgtccctcgg
     1561 ctcggccgcc agctcggccg ccgcggtcgg cgttggcgcg cgcacatcgg cgcagaaatc
     1621 cgcgatcgtg aagtcggttt cgtggccaac cccgcaaatg accggcaccg gactttgcac
     1681 gatggtgcgc gccagttgct cgtcgttgaa cgcccagaga tcctcgatcg agccgccgcc
     1741 gcgcaccagc aggatcacgt ccacggaccc cgtctcgccc aggcggtaca gcgccctgag
     1801 tgcgcggatc agttcggcgg gcgcctgcga tccctgcacg gccgccggtg ccagcgccac
     1861 cggaacatgg ggcgcacgcc gccgcaatgc ggtcaccacg tcgtggaggg cggcggcgcc
     1921 cggggaagtc accacaccga tgcccttggg cagcaccgga acgggccgct tgcgtgccgg
     1981 atcgaacagg ccctgcgcat ccagccttgc cttgagctgc aggaactgct cgaacaaggc
     2041 gcccggtcca gcccgggtca ggctttcgac gatcagttgc aggtcaccgc gcggctcgta
     2101 aatgtccaga cggcccagca cttccaccat ttcgccgtca cccggcagga agtccatcaa
     2161 cccggccgcg cgtcgaaaca tggcgcagcg gatctggccc tgcgcatcct tgagggagaa
     2221 gtaacagtgt ccgctggccg cacgcgagaa gcccgaaatc tcgccgcgga ccgccaccgg
     2281 attgaacctg gcctccaggg cgtcagcaat ggcgcggcac agggcgccaa cttgccagat
     2341 tcgcggcgcc aatggtgggt gatatggttc agccatcagc aaattcgcgg tttccggcgc
     2401 ataacgagcc caagttagtc cacagcacct cgcaatttcc cgctttgtaa cccgtgtccc
     2461 ccgccaacgc agattctgcc gagcaagtct ttgaattcat tggaattttt cctgctcaaa
     2521 ttgtcatcgt tttgtcacgt tgaaggattg gcgcggcttt gcgcgctgct ggggggccct
     2581 tctgcacaaa gttatccaca agttctgtgg gtggcctcct acaatatgtc ggcgtattcc
     2641 taggaggcgc ggagtacacg ataatcagcc gctacccggc attggcagcc gcgcggagac
     2701 cctccccttg ttttcgatca tacaagccgc aggctggccg atctggccgt tggtagcctg
     2761 ctccatcatc gccctggccc tggtcatcga gcgcttcatc agcctgaaaa ccgccaaggt
     2821 cgcgccgccc aggctgctcg acgaagcact gtcggtatcg cgcacctcgg tacccagctc
     2881 cgacgtcgtc aggcagctgg aacagaactc cgccctcggc gaggtgctgg ccagcggttt
     2941 ccgcgcgctc aactccgacc cgcgctgcag cgaggtcgac ctgcgggccc acatggaaca
     3001 taccgggcgc gaggtggcgc accgtctgga acgctatttg agtgcgctcg caacgatcgc
     3061 ctcggccgcc ccgctgctgg gcctgctggg cacggtgatc ggcatgatcg agatcttcgg
     3121 ctcgcagacg ccttccggcg gcgcgcccgg aggcaatccg gcccagctgg cgcacggcat
     3181 ctcggtggcg ctgtacaaca cggctttcgg cctgatcgtg gcgattccgt cactgatctt
     3241 ctggcgctat ttccgggtgc gggtcgacga gtacctgttg acgctggagc tctcggccga
     3301 gcgtttcgcg cgccacctca acgccttgcg tgtgcgatga acttccgacc gcgccagaag
     3361 gaagaaccgg agatcaacct gatcccgttc atcgacgtgc tgctggtggt gttgatcttc
     3421 ctgatgctga cgaccaccta cagcaagttc accgagatgc aggtgaagct gccggtggcc
     3481 gatgccgacg cgcagcgcga ctaccccaac gaagtgatcg tgggcgtcag cagcgacggc
     3541 aggtactcga tcaagggggt cccggtggcg ggccgcagcg tcgaagcggt tgcgcgcgct
     3601 ttgggcgaag cggccagcgc cggcaaggac agcgtcatca tcatcagcgc cgacgcggcc
     3661 gcgccccacc aatcggtcat cacggtgatg gaggcggccc ggcgctcggg cctggtccag
     3721 atcaccttcg ccacgcagtc atcggcgcgg gccgggagcc ggtaggccgt ggcgcaggcg
     3781 ttgcagcgcg catggctgcg gcgcggcgca ctggcccgcg tgctctggcc cctgtcactg
     3841 gcattcgggg cgcttgccgc gctgcggcgc gccgcctacc gcatgcgcct gctcgaaagc
     3901 gagcgcctgg cggtacccgt cgtcatcgtg ggcaacgtcg tcgccggcgg ctccggcaag
     3961 acaccggtgg tgatggccgt cgtcgaccac ctgcgttcgc gcggccttgc cgcgggcgtg
     4021 gtgtcgcgcg gctacgggcg cagcacttcg gattgccgcg aggtgcggcc cgacagccgc
     4081 gccgtcgagg tcggcgatga accgctgctc gtggcgcaac gctgcgaggt gcccgtgttc
     4141 gtggccgcca gccgcgccga ggcggcgcgg gccctcctgg cggcccatcc ggcaacgcag
     4201 gtgatcgtca gcgacgacgg cctgcagcac tatcgcctgc aacgcgacat cgaaatctgc
     4261 gtgttcgacg agcgcggcac cggaaacggc tggttgctcc cggccgggcc gctgcgcgag
     4321 ccctggccgc gcagcgtgga cctggtgctg agcccgggcg ccgccacggg catcgccggg
     4381 ttcgaggccc ggcgccggct ggcagcggtg gccgttcgcg gcgacggcac gcgcatcgag
     4441 ctggcctcgc tgaagggccg gcccctgaaa gccgtcgccg gcatcgccag gccggaggcc
     4501 tttttcgcga tgttgcgcgc gcagggcctg caggtcgtgc aggccgttgg gctgccggac
     4561 catcaccgct tcaatgaacc cgtcagcgcg gaatacgagc tgatctgcac cgaaaaagac
     4621 gcggtcaagc tctggcgcct ccggccggaa gcgtgggccg tgccgttgcg gctcgaggtc
     4681 gagccgcaat tctgggccgc gctcgatcgc ctgctcgatg cgaagctatc atcgcgccat
     4741 ggatcccaaa cttcttgagc tgctcgtgtg ccccgtcacc aagggccacc tggattacga
     4801 ccgcacgcgg caggagttgc tgtcgcgcag tgcgcgtctg gcttacccga tccgcgacgg
     4861 catcccgatc ctgctggagg aagaggcccg gaccttgagc gacgaggagc tggagcgctt
     4921 gcccgcgcgc acgccgctgg tttaggaacc ctgtcaagtg cacagtacca gcgtgaacgg
     4981 gccctgagcg tgggtttttg cgtcctcatc ccggcacgcc tggcctccac ccggctgccg
     5041 gacaagcccc tcgcggatat cggcgggcaa cccatggtcg tgcgggtggc ccagcgcgcg
     5101 agcgcatcgt ccgcgcgacg cgtcgtggtg gccgccgaca gtgcctcgat cgtggacgcc
     5161 tgccggaccc acggcgtcga agccatcctg acgcggggcg ttcatgcttc aggcagcgac
     5221 cgggtggccg aggcctgcga attgctgggg ctttcggggc gcgacctcgt ggtcaacgtc
     5281 cagggggacg aaccgctgat cgagccggcg ttgatcgatt cggtcgccgc gttgctggag
     5341 acccatcccg aggccgacat gagcacggcc gctcacccga tcgacagcgt gggcgacttc
     5401 accaatccga acgtggtgaa ggtggtgctc gatgtccgcg gcttcgcgct ctatttcagc
     5461 cgtgcgccgc tgccctggtg gcgcgatggt ttcgcgtcgg gaatccatgc cttgccgcag
     5521 ccccggccgt tgcggcacgt gggtatctac ggctaccgtg ccgattttct gcgtggcttt
     5581 cccaggctgg cgcccgcgcc gctcgaagtg gccgaggcgc tggagcagtt gcgcgcgttg
     5641 tggcacgggc atcggatcgc cgttcatgtg agcgcgacgg cgccgggcac gggggtcgac
     5701 acacccgagg acctggaacg cgtgcgcgcg ctgtggcgcg aggcctagcg cacaaggcac
     5761 gcatgctatc ctttgcgcaa tgatgcgcaa cggccgggtg ggtcgatacc ctggcccgca
     5821 gcgcccggcg acttcttaat ccgaggacat ccatgagact catcttgttg ggcgcgcccg
     5881 gcgcgggcaa aggcacgcaa gcggcattca tctgccagaa gtacggcatc ccgcagatat
     5941 ccaccggcga catgctgcgc gcggccgtca aggcgggcac gcccctggga cttgccgcca
     6001 ggaaagtgat ggactcgggc gctctggtca gtgacgagat catcatcggg ctggtcaagg
     6061 aacgcatcgc gcagcccgat tgcgccaatg gcttcctgtt cgacggtttc ccgcgcaccc
     6121 tgccacaggc cgatgccatg agggccgccg gggtcaagct cgattacgtg ctggagatcg
     6181 acgtgccgtt cgacgcgatc gtcgagcgca tgagcgggcg ccgctcgcac ccggcatccg
     6241 gccgcactta ccacatcaag ttcaacccgc cgaaggtcca gggcaaggat gacgtcaccg
     6301 gcgagccgct gatccagcgc gaggacgaca aggaagaaac cgtcaagaag cgcctggagg
     6361 tctatgccgc gcagactcgg ccgctagtcg agtactactc cgagtgggcc aaggccgatc
     6421 cggtcaatgc gcccaaatac cgggccatca acggcaccgg cacggtagaa gaaatcaagg
     6481 cgcgcgccct cgatgcgctt tcgagctaaa gccttgtcat tgcgaacccc ggatcaggtt
     6541 cggggcaggc ggatcgagtc cggcatgaca aggcactttt ggacgccgga tcgggtcggg
     6601 catcacaagg cacatttctg tcattgcgga ctcgatccgc aatccatgaa tcccggatgg
     6661 ggcgctttca gccaagcaac tcgagcatca gcgcgatccg ggctttgacc ggcgacaggt
     6721 gccccgcgcc gggcaaagta tcgtccggcc tggccagcac cggcccttgc gggcaccgcg
     6781 tggaccgcac gactttcaca ccgcgcgcct gggcctggcg cagcgcctcc tccaactcgc
     6841 ggcgcacgga accgttgccg gttccggcca cgacgaggcc gtccactccc tgatcgacca
     6901 gagcctgaac gatcgccgcg ccggcgccgg catggttcat gatgatttcc acgcgcggcc
     6961 agcgctggcc ggcggtgaag ttgccgaacg cccgtgcagc cccgggatcg gcggcaggcc
     7021 agtcgcgcag ccgccgcagc cggccttcct cgacatagcc gatcggtccg gcatcgcctg
     7081 aagtgaacgc atcgaggcgg taggtgtgct gcttggcgac gtccggcgcg ccgtggacgg
     7141 tcccggcaca aacggcgacc accccgtgcg cgcccgcagc gcgcgccacc gcgattgcat
     7201 cgagcaggtt ctgcgggccg tcgggcacga gcgcggtggc cggtcgcatc gcacaggtca
     7261 gcaccaccgg cttggcaggc gccagcaccg catggaggaa ataggcggtt tcttccagcg
     7321 tgtcggtccc gtgggtgatg acgacgcccc cgacatcatc ttgcgcaagc caatgcgcgc
     7381 agcgcagcgc cagcgtgcgc cagatctcgt agctcatgtc cttgctgtcg atctgggcca
     7441 cctgctccgc gatcaatggc agcgacgcga gggccggtat cgcggccagc agctcctgca
     7501 cccccatctg cgcggccgtg tagccgatat tgtcatgtgc cgcctcggcc cgcccggcga
     7561 tggtcccgcc ggttgccaga accgctattt ttttgccatt cacttgcata gtttgaaaaa
     7621 ctgtttaaaa atacaggtac tggatattaa gccagtggag tcgccgccat gctcgatacg
     7681 cccaagctca cagcccgcca gcaacagatt ctggacctga tccagagcgc cattgcgcgc
     7741 accggcgcgc cgcccacgcg cgcggagatt gcgtccgagc tcggcttcaa gtccgccaac
     7801 gccgccgaag agcatctgca ggcgctagcc cgcaaaggcg tgatcgagct ggtcagcggc
     7861 acctcccgcg gcatccggct caagagcgat gccctgcgcg ccatcaatga atcgcgcaac
     7921 aagcagttct cgctgccgct gcaaacgctg gcgcagctcg ccctgcccct gatcggccgg
     7981 gtcgcggccg gctcgccgat tctcgcgcag gagcatgtgg accagaccta ctacgtggaa
     8041 agcagcctgt tccagcgccg tcccgattac ctgctcaaag tgcgcggcat gtccatgcgc
     8101 gacgccggca tcatggacgg cgacctgctt gccgtgcagg ccaccaagga cgccaagaac
     8161 ggccagatca tcgtggcccg cctgggtgac gacgtgacgg tcaagcgctt ccgccgcaac
     8221 aagcacctga tcgagctgca cgcggaaaat cccgactacc cgaccatcat cgtcgagccg
     8281 ggtgagccgt tcgaaatcga gggcctggcc gtcggcctga tccgcaacac catgctcatg
     8341 taatcacaga gagcgccgca ggtggcgggc gtatggcgct tgaggcgcca tcaccctgcg
     8401 gtggctcccc cgtgtcaatc ctgcctgtgt tcaacctctg acgttcaagg agagtcgcat
     8461 gggaatcgca atgttgacgt tcggcgggct gctggcaccg ctgcgcagcc tggctggctg
     8521 gctggcgcac gcgccgaacg cgcggcaagg cacggaccaa gggcacctgc ccaccgctag
     8581 tttcgccgct tcaagggcct gtcctgcaag gcctgttccg cccggcagtt cccgcgagag
     8641 gccgcgccgg cctttgcggg tggtgcgggt ggtggaggct tcccgcgtgc ccgccgccgc
     8701 agggcgcatg atcatttcgg gccgaatggc cgacgtctgc gccgaactag accggctcgc
     8761 cgcgctcgag gcaacggcgc gatagtagtc agggcgagcc ggcggcgtct gtcttcaggc
     8821 gccgccgccg accaggtaat cggacttgcc cagttccaca ccgttgtgcc gaaggatggc
     8881 gtaggccatg gtgatgtgga agaagaagtt gggaagtgcc caatgcttga gataggcctc
     8941 ggccttcatg gtgcgtgtcg tttcccggcc caccggaaag gtgatgtcct tgtcttccgt
     9001 gccatccagg cgttcggcgg gcacagtccc gaggtagtcc agggtcttgc ggatgcgggc
     9061 cttcagctct ggaaaagtgg tctcgacatc ctcgaacttc ggcgcttcga cccctgaaat
     9121 gcgggccatg ccgttcttgg cggcgtcgca ggcaatctgg atctgccgcg tgaacggcag
     9181 catgtccggt gccatcctga actgcagcag cgcggcgggg tcgaattttc tggcttccgc
     9241 atgagcctgg gccttgtcga gaatatggct caggttgccg agcatggtct tgaataccgg
     9301 caagctggcg gaggacatgg aaatggtcat ctggaaaact cctgggtaac agaacgaaag
     9361 cgccgatgct aacggccttg cgccgacttt gctgccggtg tgcgctcggg gccgaggcac
     9421 aatccggtca tgaacattgt catcctcgac gattatcagg acgcggtgcg caaacttcgt
     9481 tgcgccgcca agctcgaagc ctatcccgcc aaggtctaca ccaacaccgt caagggcatc
     9541 ggccagctgt cggtgcggct gcgagacgtc gacgtgatcg tcctcatccg tgaacggacc
     9601 catatcaccc ggcaactgct ggaaaaactg ccccgcctga ggctgatcgc ccagaccggc
     9661 agggtcggcg cgcacatcga cttgcaggcc tgcaccgagc gcggaatcgc ggtggcggaa
     9721 ggcgtgggat ctccagtcgc cccggccgag ctcacctggg ccctgatcat ggcggcgatg
     9781 cggcgcctgc cgcaatacat ctccaccttg aagcacggcg gctggcagca gtccggcatg
     9841 aagtcggcct cgatgccgcc gaacttcggc ctgggcgtcg tcctcagggg ccgcacattg
     9901 ggtatctggg gctacggcaa ggtcggacag ctcgtcgccg gctatggcca ggccttcggc
     9961 atgaaggtga tcgtctgggg cagccaggac tccaggcaga aagcggtgag cgatggcgtg
    10021 caggccgcgg ccagccgcga ggagctgttc acgcagagcg acgtcctcag cctgcacgtg
    10081 cgcctgagcg acgagtcggc gggcatcgtg acactcgacg acctgagccg catgaagccc
    10141 acttcgctgc tggtgaatac gtcgcgcgcc gaactcgtcg agcacgacgc gctgatcgcc
    10201 gcgctcaacc ggggccggcc gggcatggcc gcggtcgacg tgttcgagag cgagccgatc
    10261 ctgcagggcc acgccctgct gcggctggaa aactgcatct gcacgccgca catcggctac
    10321 gtcgagatcg acagctacga gatgtacttc ggcgccgctt tcgataacgt ggtcaacttc
    10381 atcaagggaa cgccgaccaa tatcgtcaat cccggcgcgc tgcaggtgcg gcgctagtgg
    10441 aagcccaccc ccgaagcggc ccccgaatgg cgtagcccaa ttcttctaca agtgacgagc
    10501 gaaggcccgc cttgtccgcg ctgaatccac ctgcccctgc cgccgcgaaa cccgccctgc
    10561 cggcggtgct gtggccgctg ctgttcggca acttcgtcat cggcaccggt gtgatggtgg
    10621 tccccggcgc cctcaacgaa atcagcgatt cgctccaggt gtcggtggcg accgccggcc
    10681 agctgatctc cgccggcgcc ctgctcatgt gcattggcgc gccgctcttc gcggcgctcg
    10741 tggctggatg ggaccgcagg cgcctgcttg ccttgtccat ggtctggtat ggcgcgctgc
    10801 atcttgcctc catcgcggcg ccgaccttcg ccgcgctgct cccgctgcgc gtgctcacca
    10861 tggtgtcgcc ggcgattttc acgccccagg cggccgcctg cgtcggcctg ctggtgctgc
    10921 ccgagcagcg gggccgggcc atcaccttcg tctttctcgg ctggtcgctc gcatcggtgc
    10981 tgggcctgcc gatcggcgcc tacgtgggcg gcaccctcgg gtggcgcagt gccttcggac
    11041 tggtcggatt gctgagcctg ggcagtgccc tttgggtatg gcgcacgatg cccgacggcg
    11101 tcaaaccgcc ggcattgtcg gccgcggcct ggcgcgaagc cttccgttcg cgggcgctga
    11161 tgctgtgcgt ggtggtcacg gtgctgtatt cggccgcgca gttcgtgctg ttttcctatt
    11221 tcgcgccgta ttacaagctg aagatcggca tcacgcccgg cgagctgagc ctgctgctga
    11281 tgtggttcgg cgtcttcggc ttcctgggca acatggtgat gtcgcgccac atcgaccgca
    11341 tcggcgcatc gcgcgctgtg atggcgggca tcgccgcgat ggcgctgagc atgttgatct
    11401 ggccgctggg caccagcctg gccctggcgg cgctggtggc cgtcccctgg gccctgggct
    11461 gctttgcctc caactccgcg cagcaggcca ggctggtggg tatcgccccg gcgctctcgg
    11521 cgggttcgat cgcgctgaac acctcggcca tgtatgccgg ccaggcgatc ggcgccgccg
    11581 gcggcggatg gctgatcgcc agggacggga tggacctgct gcattgggcc ggcttcgccg
    11641 gcttgctgct gtcgatggcg ctgagcgcgc tggcctcgcg ctaccggcac gtgcccaggc
    11701 actgaaagcc cgcccgggac attgccataa tcgccggcat gactcttctc tttgacaccg
    11761 tgggcgtcgt cggcgtgggc gccatgggcc ggggcatcgc ccagatcgcc gcccaggccg
    11821 gcagcatcgt caagctctac gacacgcagc cgcagtcgac tgcgaaagcg cgcgacgagc
    11881 tgttcagcca gtgggaccgc ctgctcgaga aaggccggat ggacggcgcg gcggtgcagg
    11941 cctgcaagca gcggctgcaa gtcgccgagg gcctgcaggc gctagccgat tgcaatctcg
    12001 tggtcgaggc cgtggtggag cgcctggacg tcaagaagtc gctgttcgcc gagctcgagg
    12061 ccatcgtcgc gagcacagcc gtactcacga cgaatacttc atcgctttcg gtcacggcga
    12121 tcgccgccgg cctcaagcgg ccgcatcagt tcgcgggcta ccacttcttc aacccggtgc
    12181 ccctgatgaa ggtggtggag gtggtagcgg gcttgaagac ggatccggcc gtgtgcaaac
    12241 gcctggccga atacgccggc cagatgggcc acacagccgt gcaggcgcag gacacgcccg
    12301 gcttcatcgt caatcacgcg ggccgcggct acggaacgga agcgctgcgg ctggtgggcg
    12361 aaggcgtggc ggacttcgcg acggtcgatc gcatcctgaa ggaccaggcc ggcttcaagc
    12421 tgggaccgtt cgaactgatg gacctcacgg ccctggatgt gtcgcatccg gtgatggagt
    12481 ccatctaccg ccagtactac gacgagccgc gctaccggcc cagcgtcatc acagcccagc
    12541 ggctcgcggg tggcgtggtg ggcaagaagg tgggggaagg tttctaccgc tatgtggacg
    12601 gcaaggccca ggtcgacgcc gagccggcgg cgccgcaagt ggccgagatg ccgcccgtgt
    12661 gggtgtcgac gcgcgcggcg cgccgctccg agctgtacca gctgctgaag gatctgggcg
    12721 cgaaaatcga aaccgggcag tcgccctcgc cccaggcgct gtcgctggtg gcgccgctcg
    12781 gcttcgacgt caccacggtc gcggtggtgg aacggctcga tcccgctcgc accctgggca
    12841 tcgacatgct gatcgacgac gcggccacca aacgccgcgt gctcgccacc aacccggcga
    12901 cccgcgccga catgcgcgac gcggcccacg cgctcttcgc ccgcgacggc aaggcggtga
    12961 gcgtgatccg cgattccggt ggtttcgtca cccagcgcgt ggtggccatg atcgtgaaca
    13021 tcgccgcgga catctgccag cagcgcatct gcagccccag ggatctcgag accgcggtca
    13081 cactgggact gggctacccg ctcgggcccc tggccatggg cgaccgctgg gggcccacca
    13141 acgtgctgga ggtgctcttc aacatgcaaa ccgtttacgg cgatccgcgt taccgcccca
    13201 gcccctggct gcgccgccgc ggggcgatcg gtttatccct gctccacgag gaaacgtgac
    13261 ggtggcggcc gaactcaaaa gcacgagcca gggccgcacc atggtcctga ccctgagcaa
    13321 ccccgaacac cgcaatgccc tggggccgga gatgtacgcc gcgggcgtgg aggcgctcag
    13381 cgtcgccgag agcaacccgg aggtgcgcag cgtggtgatc gccggcgaag gcaaggtctt
    13441 ttcggcgggc ggcaatctcc agcgtctgca agccaaccgg cagctgccgc cacaggtcca
    13501 ggcgcaaagc gtcgaaagcc tgcacagctg ggtcgaagcc atccggactt ttcccaagcc
    13561 ggtgatcgcc gctgtcgaag gggcggcggc cggggccggc ttctcgctgg cgctggcctg
    13621 cgacctgatc gtggcagcgg acgacgcgat cttcgtcatg gcctacatca atgtcgccct
    13681 ctcgccggac ggcggcgcca gctggagcct ctcgcaggcg cttcccaggc agctggtcgg
    13741 cgagctgctg ctgggcggcg aacgcatgac cgcacggcgg ctgcatgagc ttggcgtggt
    13801 caaccgcgtc acaccgggcg gcggtgcgct ggatgaagcg atggcgctgg cggaaaagct
    13861 caatgcgaag gcgccgaacg ccgtggcaag catgaaggag ctcatcaacg acgcggccgg
    13921 caccaccttg aaccagcagc tggcggccga acgcgaccat ctggtgagaa acctgcacca
    13981 tgccaatgcc ggcatcggca tcgcggcctt cctggccagg cagacccccg actaccagta
    14041 gcccccggca gcgcagtgcc tagggacgac cctgttgttc ccccggtccc gcacgcgaca
    14101 atgatcgcgc cccccagtca ctcaaaagac agatcatgga cgaccccatt cttaccattg
    14161 acgaacgaga ggcgatcaat gccggtcgct ggttttcatc cctttcccca tccctcaggc
    14221 acgacattct ccgatgcgcc tacgtgaaac gctacaagga cggcgagctc atctcggcac
    14281 ggggcgaccc gtccgaggaa tggatcgcct gtgccaaggg cgcggtgcgc gtgagttcga
    14341 cctcgatctc gggcaagcag gtgacattga cttacgtcga gcccggcatc tggttcgggg
    14401 atgtctcgat cttcgacggc gagcggcgta cgcacgatgc gtatgcgcac ggcgacacca
    14461 cgatcctgtg cgtggcccgc ggcgacctgc gcaagattct cagccagcac gtggagcttt
    14521 acgaggccct gctgaggctg cacgcgcggc gcatccggca gctgttcggc ctggtggagg
    14581 acctcaacac cctgcccttg cgcgcccggc tggccaagca gctcacgcac ctggtgcgca
    14641 gctacggcgt tccgtcgctg tcggacgggc gcgaggtgcg catcagcctg cagctggccc
    14701 aggaagaact ggcgcagctg ctgggggctt cgcgccagcg ggtgaatcag gaactcaagg
    14761 ccatggagcg cgacgacgtg atccgcatcg agccgggtgg gctggtggtg cgcaaccgcg
    14821 aagcgctgat gcgcatcatc gaggcggaca actgacgtcc gaaggacgtc atcccggcgc
    14881 aggccgggat ccagaaacca atagcaaagg acgtcatccc ggcgcaggcc gggatccaga
    14941 gatcaacaaa tgagcgactt cgaccatttc gtaggcaccc gccccgtttc cggcaagcac
    15001 gtcttcgata ccgcggcgct cacggcctgg ctcgagaaga acctggaggg cttcgccggc
    15061 ccgatcggcg tcgaaatgtt caaggggggc caatccaatc ccacctacaa gcttgtgacc
    15121 cccggccaga gctacgtcat gcgcgccaag cccggcccgg tggccatgct gctgccgtcg
    15181 gcgcacgccg tggaacgcga attcgccgtg atgcgcgggc tcgccggcac cgacgtgccg
    15241 gtgccacgga tgtattgcct gtgcgaagac gaatccgtca tcggccgcgc cttctacatc
    15301 atggagttca tgcagggccg ggtgctctgg gaccaggcgc tgccggagat gagcccgcgg
    15361 gggcgcggcg agatctacga cgagatgaac cgcgtgatct cggcgctgca tacggtgaag
    15421 ttcgccgagc gcggcctggc gaactacggc aagcccggca actacttcga ccgccagatc
    15481 ggtcgctgga gcaagcagta caaggcgtcg gccgatggcg ccggtgccat gagccagccc
    15541 atcccggaga tggaaaggct ggtggagtgg ctgccggccc acattccggc ggcggggcgc
    15601 gacgacagca aggtctccat cgtccacggc gactatcgcc tggacaacct gatgttccat
    15661 cccgggcagc cgcgcgtgat cgcggtgctc gactgggagc tttcgacgct gggccatccg
    15721 ctggccgact tcagctacca ctgcatggcc tggaacattc cgcacgggtc gttccgcggc
    15781 atcggcggcc tggaccatgc ggcgctggga attccatcgc aggaagagta catacaccgt
    15841 tattgcgagc gcaccggcct ggccgacgcc ggcgacttgc gtgcggactg gaacttctac
    15901 atggcctaca acatgttccg catcgccgcg atcctgcagg gcatcgccaa acgcgtcgaa
    15961 gccggcacgg cttccagcgc gcaagcggcc agctccggcg ccagcgcgcg gcccatggcc
    16021 gagctggcct ggaagttcgc gcagcaggcc tgatcacacc acgactaatt acccacagga
    16081 aagaaccatg gatttcgaat actcacccaa gacgcgcgaa cttcagacca agctgctgaa
    16141 gttcatggac gactacatct atccggccga agtgcaatac cagcaggagc tggaagccaa
    16201 caccaaggcc ggcaagcggt ggagcgcgct gcagacgatc gagaagctca agcccaaggc
    16261 ccgcgctgcc ggcctgtgga acctgttcct gccggtggac agttcctcgg cttccggcta
    16321 cgagggtgcc ggcctcacca accaggaata cgcgccgctg gctgaaatca tgggccgggt
    16381 gatgtggtgc agtgaggtct tcaactgctc ggcccccgac accggcaaca tggaaacgat
    16441 cgcccgctac ggctccgaag aaatcaaggc acgctggctc aagcccctgc tcgaaggtga
    16501 aatccgctcg gctttcgcca tgaccgagcc ggaagtcgct tcgtcggacg ccaccaacat
    16561 ctgcacccgg atcgagcgcc atggcgacga gtacgtgatc aacggccaca agtggtggat
    16621 ttccggcgcg gccgatcccc gttgcgccgt gttcatcacc atgggcaaga ccgatccgca
    16681 ggcgcctcgg cactcgcagc aaagcatggt catcgtgccg gccgacacca agggcatccg
    16741 catcgagcgg ccgctgacgg tgtacggcta tgacgacgcc ccgcacggcc acgtcgagat
    16801 gtacttcgag gatgtgcgcg tgcccgtctc caacatcctg ctgggtgaag gccgcggttt
    16861 cgaaatcgcc cagggccggc tcggcccggg acgcatccac cattgcatgc gaatgatcgg
    16921 gctggccgag cgctcgctcg agctgatgtg caagcgcgcc agctcgcgcg tggccttcgg
    16981 caagaccgtg gcccagcagt ccgtcaccca ggaacgtatt gccgaggccc gctgcaggat
    17041 cgacatggca cgcctgctca ccctcaaggc cgcatggttg atggacgtgg ccggcaacaa
    17101 ggcagccaag accgagatcg cgatgatcaa ggtggtggcg cccaccatgg cgctccaggt
    17161 gatcgactgg gccatccagg tccatggcgg cggcggcgtt agcggcgatt tcccgctggc
    17221 gcactcctgg gccgcggccc gcacgcttcg ctttgccgat ggcccggatg aagtgcatcg
    17281 catggcagtc gccaaatggg aactgggcaa gtacggctcg cccagccgcg aggccgcggc
    17341 gtcacgcggg taaggacagg tcaggcgcga tcggccccgc cagggatccc agggccgact
    17401 cgcaggcgct ggtgaatatc aaggccgcgt gccgcagcgt ggcgaggtcc gccggcgagt
    17461 actccatgcg caggtaagcc ttgccgcgca ggagcagcat cacgaaaggc acttcggccg
    17521 acggcgccgg ctcgggccag tccagcatca gccgggccag cgccggatcg acccaggcca
    17581 gtgcgctttc gcgctggtcc gacatcaccg cgtagcgctc ccagaactcg gccggcaggc
    17641 tttcccagcc gacctcctcg tacatcgcga gccaccgcat ctcctcgggc aggttcgagt
    17701 cgatcgaggt ttgaaggttg tccgtgtaga tctggtaagc ctgtttctcg agcgcttcct
    17761 tcagcggtcg gctcatcacc acgatcgcca catcttcatc gatgcccaac tccgcgcgcg
    17821 cccggacttc atcgccacgg atgtactggc gcgacgggct tcccagttgc agccgccacg
    17881 gtttgccact gaccttgccc tccagcgaga cgccgtgacc ggtgcggtcc accgagaagc
    17941 cgaagccctg cgtcgccgcc cattccgaca cggggccatg caccggatcc gaagtcgacg
    18001 cggccgcttc gaccggttcc ttcgcgtcgc tggagaaggc cttcctgatg cgatcgaaca
    18061 tggggagcgc gtttcaccta aagtcgcatt attagccagt atgcgcgaaa ggcccccgat
    18121 gatcgacgtt ttttcctggc ccactcccaa tggccacaag gtccacatca tgcttgagga
    18181 gtgcggcctt gcgtaccgtg ccattcctgt cgacattggc gcaggcgacc agttcaagcc
    18241 cgagttcctg aagatcagtc ccaacaacaa gatacccgcc atcaccgatc cggacggcct
    18301 cgacgggaaa ccgatttcgc tattcgaatc gggcgcgatc ctggtgtatc tcgcagccaa
    18361 gaccggcctg ttcctgccgc gaaccgatcg cgcgaagtac gaggtgctgc agtggctgat
    18421 gttccagatg ggaagcgtgg gccccatgct tgggcagaac caccacttcc ggctatacgc
    18481 ccccgaaaag ctcgaatatg ccgtccagcg ctacaccaac gaagcgaaac gcatttacgg
    18541 cgtgatcgac cagcgcctct cgcagaacga atatcttggc ggcagccagt attcgatcgc
    18601 cgacatcgcc acttttccct ggctgcgcag ctgggagagg cagggcatcg tgctgggcga
    18661 atacccgtat ctgcaggact ggttcaacaa gatggccgag cgcccggcgg tacagcgcgg
    18721 agtcagggtg atggcggagc tgcggcgtcc catcacgggc gagaaggaac gcgaaatcct
    18781 gttcggcaag acgcagtatg agcggcactg aaaaggcgcc gagggcacct tccggggctg
    18841 cacgcgagcc tgacagctcc gatcaaccgc gtggcccgcc cgggacgttt gtcccagatt
    18901 aattgagcaa cctgtccctt tcccacgctc ctgagcgcac ctaagctggg cgcaccagaa
    18961 tcctgaaggg agcgaaaaat gaaacgtgcc gccgcagtcg agatttcgcc gtgcgacccc
    19021 cctgtcctgg gcgatctgtc gctggagcct ttcagcccgc acgattgg
//
LOCUS       DASWIY010000226         8833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2730831, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000226 DASWIY010000000
VERSION     DASWIY010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8833
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2730831"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VGA59_08335"
     CDS             complement(<1..197)
                     /locus_tag="VGA59_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719725.1"
                     /translation="MIAILLSSTGGCCHCGLAWMPDRVRHDKFGCHCGLDPQSRLFSF
                     SHASSVSSSAPSTTWSPGANST"
     gene            196..1116
                     /locus_tag="VGA59_08340"
     CDS             196..1116
                     /locus_tag="VGA59_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEX9719726.1"
                     /translation="MRISFCCTNTKAAPWLQGLREALPQASVEEWRPGAPQADHAVVW
                     APPQQFLDEQPRLRAIFNIGAGVDALLKLKVPPEALVVRIDDGGMAVQMAEFVCHAVI
                     RHFRELDAYDVDVKQGKWSFRRPRDRSDFPVGIMGLGVLGQRVARAVAHFEFPVLGWS
                     RTARSVPAVRCYAGQEQFGDFLAGTRILVCLLPLTPETNGIMNAQNLSRLMPGGYVIN
                     VARGAHLVDDDLIALLDSGHLAGAALDVFRTEPLPADHPFWRHPKITVTPHTSARTLR
                     DESVAQIAGKILALERGEPIAGVVDVKRGY"
     gene            1122..2030
                     /locus_tag="VGA59_08345"
     CDS             1122..2030
                     /locus_tag="VGA59_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX9719727.1"
                     /translation="MKLPSKVKIVDVGPRDGLQNEKEPVPAEVKIALVHRLQDAGLKE
                     IEVTSFVSPKWVPQMADNAEVMAGIERKPGVRYSVLTPNMKGFEAAVASRPDEIVVFG
                     AASEAFSQRNINCSIAESIERFAPVVEAARARGIHVRGAISVALGCPYEGEVAPERVE
                     LVARLMKEIGVQHIGVADTIGVGTAVKAQRAMEAALKHYAIDDISGHFHDTYGQALAN
                     TLAALEMGIWQYDTSVAGLGGCPYAKGATGNVATEDVVYMLHGMGIATGIDLDKLIDA
                     GKSISDYLKRKPNSRAATAILNKRAG"
     gene            2037..2552
                     /locus_tag="VGA59_08350"
     CDS             2037..2552
                     /locus_tag="VGA59_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX9719728.1"
                     /translation="MCGAEVSLLPEGVQRVATALREQGHPHAPLMLDDAARTAQQAAD
                     QLGIAVGQIAKSIIFRRKSDDAAVLVITSGDRRVDEKKVAALVGQGGSLGRADADFVK
                     SRTGYSIGGVSPIAHAQPPVTLIDRELFRFEEIWAAAGHPHGVFKLRPQDLERLTGAP
                     VADVVQQPASA"
     gene            2561..2788
                     /locus_tag="VGA59_08355"
     CDS             2561..2788
                     /locus_tag="VGA59_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEX9719729.1"
                     /translation="MQRLVQRARHVGAQSTPDVPSPCVSVCRMDPVTELCEGCFRTLD
                     EIAWWARMEDESKREVWRMIEERALTQAAMD"
     gene            3003..3671
                     /locus_tag="VGA59_08360"
     CDS             3003..3671
                     /locus_tag="VGA59_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX9719730.1"
                     /translation="MKQITFYLDFVSPYAYLAFEKLPETLLGLSNAVDYKPALFAGML
                     KHHGQLGPAEIMPKRDWTYRQVLWLAHSHRIPMQMPAAHPFNPLPLLRLALACAGQDS
                     LKAFGPGLANRYVCETIFHHVWRGGADAVDPQRLDALKLLLQPQRDPAGEGVKDELKA
                     NTDEAIARGVFGVPAFAVDDKLFWGFDALPMLRAYLEGDAWFGQGHWETAAQRPGQQR
                     RTVS"
     gene            complement(3702..6275)
                     /locus_tag="VGA59_08365"
     CDS             complement(3702..6275)
                     /locus_tag="VGA59_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719731.1"
                     /translation="MRAPCNRRILLIDDMPSIHEDFRKILESRSSTRNLDDAEAALFG
                     EAAPASGESFELDSAYQGREAAVMVATAVQAGRPYAMAFVDMRMPPGWDGVETIERLW
                     RIDPRLQVVICTAYSDHPWEEVMARLDVQDRLLVLKKPFDMIEVSQLARMLTAKWELA
                     RQAASRISGLEQAVQERTGELTAAKLAAEHANQAKSDFLANMSHEIRTPMNAVIGLSH
                     LVLKTDLTPRQRDYITKVQTSGHYLLGVINDILDFSKVEAGKLAIENTEFELEKLLDH
                     ASSLISEKGHAKGLELVFDVAPDVPRHLVGDSLRLGQILINYANNAVKFTEKGEIVIA
                     VRASERTDKAVLLHFRVQDTGIGLTPEQMGRLFQSFSQADASTTRKFGGTGLGLAICK
                     KLAELMGGEVGVQSECGKGSTFWFSARLGIGKAVPRELVPNPDLRGRRALVVDDNAHA
                     RAALVDMLEGMSFLATEVSSGATAVQEVRRAAASGHPYDIVYLDWLMPGMDGMDTARR
                     VKALGLAASPLFLMVTAHGREAVLKEAELVGIDSVLVKPVNASILFDTTMEALGGRLA
                     KPSAAVDTPQAVNSRLAAIQGARILLVEDNDINQQVVRELLEDAGLAVDVAENGEVAL
                     GMVGNTAYDLVFMDMQMPVMDGLTATRAIRKMGRLKHLPIVAMTANAMEQDRQKCLAA
                     GMNDALIKPIDPERISDMLLKWIKPRAAAQQSVARTTPAIPDFPETIDGLDIHAGLGR
                     MLGKKPQYLKLLRMYVAGQRRVPEEIRTTLDNNDWATAERLAHTTKGVSGSIGALRIP
                     DHAGELEHAIRERKPRGEIDRLLRAMEIPLGQLIATLETQLPPEPAGAVAGSAPQRTT
                     A"
     gene            complement(6272..7942)
                     /locus_tag="VGA59_08370"
     CDS             complement(6272..7942)
                     /locus_tag="VGA59_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX9719732.1"
                     /translation="MPPIKKPLPALYAAGRVDPAQALLEVTAIFENASVGIFITRDSV
                     VHRCNLRAAEIFGYSSPEELIGQPTAVIYPDVESFHRMGREAGPVLVTGRSFRTDWAF
                     RKTDGTEVWCRVDGKPLDPQCTSAGTAWVIDDITERRRADQALHDSKAVLDDTLAYMD
                     QGISLFDNDLKVLATNRRYFELLDFPESLAAPGTPFADFIRYNAQRGDYGPGDVEEQV
                     RSRVEKAARFEPHHFERQRPDGTVIEVRGLPVPGRGFVTIYTDITKRAQAEQALRETQ
                     TELVATARQAGMAEIATNVLHNVGNILNSVNVSAGLISGTLRKSRVQGLTKAAQLMND
                     HAADLGDFLTRDDKGKMLPRYLSEIAQTLAQEQQAMLEELAHLTRSVDHIKAVVATQQ
                     SYAGRASIVEPVQMRELMEDALRINGDAQAHDRMTIVKEFAEVPVSRLDRGRVLQILV
                     NLISNAKHAMEGVADRPRRMTLRLETAGPSLRVCVQDEGEGISATNLTRIFSHGFTTR
                     KNGHGFGLHSCALAAGQMGGALTAHSDGPGKGATFTLELPIDGTRAAS"
     gene            8354..8614
                     /locus_tag="VGA59_08375"
     CDS             8354..8614
                     /locus_tag="VGA59_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEX9719733.1"
                     /translation="MKVDTNGEAYWKATLRLLTRILIIWFLVSFGAGILFADLLNNIK
                     LGGYPLGFWFAHQGSIYVFIALIFYYARKMGQLDRQFDVHEE"
     gene            8625..>8833
                     /locus_tag="VGA59_08380"
     CDS             8625..>8833
                     /locus_tag="VGA59_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX9719734.1"
                     /translation="MELQTTIYIVVGLSFALYIGIAVWARAGSTSEFYAAGGSVHPVM
                     NGMATAADWMSAASFISMAGLISNMG"
BASE COUNT         1417 a         2905 c         2913 g         1598 t
ORIGIN      
        1 gtgctgttcg cgcccggcga ccaggtggtg gagggggccg aactgctgac gctggaagcg
       61 tgagagaagc tgaacagcct ggattgcggg tcgagcccgc aatgacaacc gaatttgtca
      121 tgccggaccc gatccggcat ccatgcgagc ccgcaatgac agcacccacc cgtcgatgac
      181 agcaaaatgg cgatcatgcg catttcattc tgttgtacca ataccaaggc cgccccgtgg
      241 ctgcaaggac tgcgcgaagc gctgccccag gcgagcgtcg aggaatggcg accgggcgcg
      301 ccgcaagccg accacgccgt ggtgtgggcg ccgccgcagc agttcctcga cgagcaaccg
      361 cggctcaggg ccatcttcaa catcggggcc ggtgtcgatg ccttgctgaa attgaaagtg
      421 ccgcccgagg cgctggtcgt gcgcatcgac gacggcggca tggcggtgca gatggccgaa
      481 ttcgtctgcc atgcggtgat ccggcatttc cgcgaactcg acgcgtatga cgtggacgtg
      541 aagcagggca agtggtcgtt ccggcggccg cgcgaccgca gcgactttcc cgtcggaatc
      601 atgggcctgg gcgtcctggg ccagcgagtc gcccgggccg tggcgcactt cgaattcccc
      661 gtgctcggct ggagccgcac ggccaggtcg gtgcccgccg tgcgctgcta tgcgggccag
      721 gaacagttcg gcgatttcct cgccggcacc cgcatcctgg tctgcctgct gccgctcacg
      781 cccgagacga acgggatcat gaacgcgcag aacctgtcgc ggctgatgcc gggcggctac
      841 gtgatcaatg tggcgcgagg cgcgcatctg gtggacgacg acctgatcgc actgctggac
      901 agcgggcacc tggccggcgc cgcgctggac gtgttccgca ccgagccctt gccggcggac
      961 catccgttct ggcgtcatcc caagatcacg gtgactccgc acacctcggc gcgcacgttg
     1021 cgtgacgaga gcgtcgccca gatcgccggg aaaatcctcg ccctggagcg gggagagccc
     1081 atcgccggtg tggttgacgt gaagcgagga tactagctgc catgaaactc cccagcaaag
     1141 tgaagatcgt ggacgtgggc ccgcgcgacg ggctgcagaa cgaaaaggag ccggtccccg
     1201 ccgaggtgaa gatcgctctg gtgcatcgcc tgcaggacgc gggcctcaag gaaatcgagg
     1261 tcaccagctt cgtctcgccc aagtgggtgc cgcagatggc ggacaacgcc gaggtcatgg
     1321 ccggcatcga gcgcaagccg ggcgttcgct attcggtgct cacgcccaac atgaagggct
     1381 tcgaggcggc cgtggcgagc cggcccgacg agatcgtggt gttcggcgcc gccagcgagg
     1441 cgttcagcca gcgcaacatc aactgctcga tcgccgagag catcgagcgc ttcgcacccg
     1501 tggtcgaggc cgcaagggcc aggggcatcc acgtgcgcgg cgccatctcg gtggcgctcg
     1561 gttgccccta cgagggcgaa gtcgcccccg agcgggtgga gctggtggcg cgcctgatga
     1621 aggagatcgg cgtgcagcac atcggcgtgg ccgacacgat cggcgttgga acagcggtga
     1681 aagcccagcg cgccatggag gcggctctca agcactacgc gatcgacgac atctcgggcc
     1741 acttccacga tacctacggg caggcgctgg ccaacaccct ggcggcgctg gagatgggca
     1801 tctggcagta cgacacgtcg gttgccggcc tgggcggctg cccgtacgcc aagggggcga
     1861 ccggcaacgt cgcgaccgag gacgtggtgt atatgctgca tggcatgggc attgcgaccg
     1921 gcatcgacct cgacaagctg atcgacgcgg gcaagtccat cagcgactac ctgaagcgca
     1981 agccgaattc gcgcgccgcg acggcgatcc tgaacaagcg ggcggggtga tccgggatgt
     2041 gtggtgccga agtctccttg cttcccgaag gcgtgcagcg cgtggccacg gcgctgcggg
     2101 aacaaggcca tccccatgca ccgctgatgc tcgacgacgc cgcgcgcacc gcgcagcagg
     2161 ccgccgacca gctgggcatc gccgtcggcc agatcgccaa gagcatcatc ttccggcgca
     2221 agtcggatga cgccgccgtg ctggtcatca cctcgggcga ccggcgcgtg gacgagaaga
     2281 aagtcgccgc cctggtggga cagggtggat ccctgggccg cgccgatgcc gatttcgtga
     2341 agagccgcac cggctattcg atcggcggcg tctcgccgat cgcgcatgcg cagccgccgg
     2401 tgacgctgat cgaccgcgag ctgttccgct tcgaggagat ctgggccgcg gccggccacc
     2461 cgcacggcgt gttcaaactg cgaccgcagg acctggagcg cctgaccggc gcgccggtgg
     2521 ccgacgtggt gcagcagccg gcgagcgctt gatgggcgcg gtgcaacgcc tcgtccagcg
     2581 cgcccggcac gtgggtgcgc agagtacgcc cgatgtgccg tcgccgtgcg tttcggtgtg
     2641 ccgcatggat ccggtcaccg agttgtgcga gggctgcttt cgcacgctgg atgaaatcgc
     2701 ctggtgggcg cgcatggagg acgagagcaa gcgcgaggtt tggcgcatga tcgaggaaag
     2761 agcgctgacg caggccgcca tggattgagg caagcccgca atgacaaaac acttgtcatg
     2821 ccggactcga tccggcatcc atgacttcct ggattgcggg gccgggccgc agcgtgatgc
     2881 ctcggcacgt gcatagcgta ggcccggcat tgggctcgct acactggtca gctgcgcgcc
     2941 ccgggcgttc cgcgagccca gcccgcaatg acagcatagg ggcagaggga ggaagacaac
     3001 acatgaaaca gatcaccttc tatctcgact tcgtttcgcc ctatgcctac ctggcgttcg
     3061 aaaagctgcc cgagacgctg ctcgggctga gcaatgccgt cgattacaag ccggcgctgt
     3121 tcgcgggcat gctgaagcac catgggcagc tcggccccgc cgagatcatg cccaagcgcg
     3181 actggactta ccggcaggtg ctgtggctgg cgcacagcca tcgcatccct atgcagatgc
     3241 cggccgcgca tcctttcaat ccgctgcctc tattgcggct ggcgctggcc tgcgccggcc
     3301 aggattcact gaaggcgttc ggaccgggcc tggcgaaccg ctatgtctgc gaaaccatct
     3361 tccaccacgt ctggcgcggc ggcgccgacg ccgtcgaccc gcagcggctc gatgcgttga
     3421 agctgttgct ccagccccag cgcgaccccg cgggcgaggg cgtgaaggac gaactcaagg
     3481 cgaacaccga cgaggccatc gcgcgcggcg tgttcggcgt gccggccttc gctgtcgacg
     3541 acaagctgtt ctggggcttc gatgcgctgc ccatgctgcg tgcctatctc gaaggcgacg
     3601 cgtggttcgg ccagggccat tgggaaaccg ccgcgcaacg gcccgggcag cagcggcgaa
     3661 cggtttcctg atcgccaggc ttcaggccgc ctcgggccgc ctcaggccgt ggttcgctgc
     3721 ggggccgagc cggcgactgc ccccgccggt tcgggaggca attgcgtttc cagtgtggcg
     3781 atcagttgtc ccaacgggat ctccatcgcg cgcagcaatc ggtcgatctc gccccgtggc
     3841 ttgcgttccc tgatcgcgtg ctcgagttcg cccgcgtggt cgggaatccg cagcgcgcct
     3901 atgcttcccg atacgccctt ggtggtgtgg gcgagccgct ccgccgtcgc ccagtcgttg
     3961 ttgtcgagcg tagtgcggat ttcctcgggc acacgtcttt gcccggcaac atacatgcgc
     4021 aggagcttca ggtactgcgg cttcttgccc agcatccggc caagacccgc atggatgtcg
     4081 aggccgtcga tggtttcagg gaaatccggg atcgccggcg tcgtccgggc gacgctctgc
     4141 tgcgcggctg cgcgcggctt gatccacttg agcagcatgt ccgagatccg ctcggggtcg
     4201 atcggcttga tcaaagcgtc attcatgccc gcggccaggc atttttgccg gtcctgctcc
     4261 atggcgttgg ccgtcatggc gacgatggga aggtgtttca gccggcccat cttgcggatg
     4321 gcccgggtgg ccgtcagccc gtccatgacg ggcatctgca tgtccatgaa caccaggtcg
     4381 tatgccgtgt tgcctaccat tcccagcgcg acctcgccgt tttccgccac gtccaccgcc
     4441 aggccggcgt cctccagcag ctcgcgcacc acctgctggt tgatgtcgtt gtcctccacc
     4501 agcaggatgc gggcgccttg aatggccgcc agccggctgt tgaccgcctg gggcgtgtcg
     4561 accgccgcgc tcggcttggc cagccggccg cccagcgctt ccattgtcgt atcgaacagg
     4621 atcgaggcat tgaccggctt gaccagcacg ctgtcgattc ccaccagttc ggcctccttc
     4681 agcactgcct cgcggccatg ggccgtcacc atcaggaaca acggcgaggc ggccaggccc
     4741 agggccttga cgcgccgcgc cgtatccatc ccgtccatgc cgggcatgag ccagtcgagg
     4801 tagacgatgt cgtacgggtg gccggaggca gcggcccggc gcacctcctg caccgcagtg
     4861 gcgcccgacg agacctcggt cgcaagaaag ctcatccctt cgagcatgtc caccagggcg
     4921 gcgcgggcat gagcgttgtc atccaccacc agggcgcgcc gcccgcgcag gtcggggttg
     4981 ggcaccagct cgcgcggcac ggccttgccg atgcccagcc gggcactgaa ccagaaggtg
     5041 ctgcccttgc cacattcgct ttgcaccccc acttccccgc ccatcagctc ggccagcttc
     5101 ttgcagatcg ccagacccag cccggtgccg ccgaacttgc gcgtggtcga agcgtcggcc
     5161 tgcgaaaaac tctggaacag ccggcccatc tgctcgggcg tcaggccgat gccggtgtcc
     5221 tgcaccctga agtgcagcag cactgccttg tcggtgcgct cgctggcacg caccgcgatg
     5281 acgatctccc ctttttcggt gaacttcacc gcgttgttgg cgtagttgat caggatctgg
     5341 cccaggcgca gcgaatcgcc caccaggtgg cgcggcacat cgggcgccac atcgaacacc
     5401 agctccaggc ctttggcatg gcctttctcg ctgatcaggc tgctggcgtg gtccagcagt
     5461 ttctccagct cgaattcggt gttctcgatt gccagcttgc cggcctcgac cttggagaag
     5521 tccaggatgt cgttgatcac acccagcagg taatgccccg aggtctgcac cttggtgatg
     5581 tagtcgcgtt ggcgcggcgt gagatcggtc ttgagcacca ggtgcgacag gccgatgacg
     5641 gcgttcatcg gcgtcctgat ctcgtggctc atgttggcca ggaagtcgct cttggcctgg
     5701 ttggcgtgtt cggccgcgag tttggcggcg gtcagctccc cggtgcgttc ctgcacggcc
     5761 tgctccaggc cgctgatgcg cgaagcggcc tggcgggcca gttcccattt cgccgtcagc
     5821 atcctggcca gctggctgac ctcgatcatg tcgaacggct tcttcagcac cagcaggcgg
     5881 tcctgcacat cgaggcgggc catcacttct tcccacggat ggtccgagta ggcggtgcag
     5941 atcaccacct gcagccgcgg gtcgatgcgc cagagccgct cgatcgtctc cacgccgtcc
     6001 cagcccggag gcatgcgcat gtccacgaag gccatggcat acgggcggcc cgcctgcacc
     6061 gctgtcgcga ccatgaccgc cgcttcgcgc ccctgatagg ctgaatccag ctcgaagctt
     6121 tcgccggacg ccggcgcggc ctcgccgaac agggcggctt cggcgtcgtc caggttgcgc
     6181 gtcgaagacc ggctctcgag gatcttgcga aagtcctcat ggatcgaggg catgtcgtcg
     6241 atcagcagga tgcggcgatt gcacggcgct ctcatgaggc ggcccgtgtc ccgtcgatcg
     6301 gaagctccag cgtgaaagtc gcgcccttgc ccggaccgtc actgtgggcc gtgagggcgc
     6361 cgcccatctg ccccgctgcc agggcgcagc tgtgaagccc gaagccgtgg ccgttcttgc
     6421 gcgtggtgaa tccatgcgag aagatgcgcg tcagattcgt ggccgagatg ccttcgccct
     6481 cgtcctgcac gcatacgcgc agactgggcc cggccgtttc cagccgcagc gtcatccggc
     6541 gcgggcgatc ggcaacaccc tccatcgcat gcttggcatt gctgatcagg ttgaccagga
     6601 tctgcagcac ccgtccccgg tccagccgcg aaaccggtac ttccgcgaac tccttcacga
     6661 ttgtcatccg gtcgtgcgcc tgcgcgtccc cattgatgcg cagcgcgtct tccatcagct
     6721 cgcgcatctg taccggttcg acgatgctgg ccctgcccgc gtacgattgc tgggtggcga
     6781 cgacggcctt gatgtgatcg acgctccggg tgaggtgggc cagttcctcg agcatcgctt
     6841 gttgctcttg cgccagggtt tgcgcgattt cgctcaggta gcggggcagc atcttgccct
     6901 tgtcgtcgcg ggtcaggaaa tcgcccaggt cggccgcatg gtcgttcatc aactgcgcgg
     6961 ccttcgtcaa accttggacc ctggacttgc gcagcgtgcc gctgatcagg ccggccgaga
     7021 cgttgacgct gttgaggatg ttgcccacgt tgtgcagcac gttggtggcg atctcggcca
     7081 tgcccgcttg gcgggcggtg gcgaccagct cggtctgcgt ctctcgcagc gcctgttcgg
     7141 cctgggcccg cttggtgatg tcggtgtaaa tcgtcacgaa tccgcgcccg ggcacaggca
     7201 ggccgcgcac ctcgatcacc gttccgtcgg ggcgctggcg ctcgaagtgg tgcggctcga
     7261 atcgcgccgc cttctcgacg cggctgcgca cctgctcttc gacgtcgccc ggcccgtagt
     7321 caccgcgctg cgcgttgtag cggatgaaat cggcgaaagg cgtgcccggc gcggccagcg
     7381 actccggaaa gtcgagcagt tcgaagtagc gccgattggt tgccaggacc ttcaggtcgt
     7441 tgtcgaacag gctgatgccc tggtccatgt atgcgagcgt gtcgtcgagc accgccttgc
     7501 tgtcgtgcag tgcctggtcc gcgcgccgcc gctcggtgat gtcgtcgatc acccaggctg
     7561 tgccggcgct ggtgcactgc ggatcgagcg gcttgccgtc gacccggcac cagacctcgg
     7621 tgccatcggt cttgcgaaac gcccagtcgg tgcgaaacga gcggcccgtc accagcaccg
     7681 gcccggcttc gcggcccatg cgatggaagc tctcgacgtc ggggtagatc accgccgtgg
     7741 gctggccgat cagctcctcg gggctgctgt agccgaagat ctcggccgcg cgcaggttgc
     7801 agcgatgcac cacgctgtcg cgggtgatga agatgccgac cgaggcgttc tcgaaaatcg
     7861 cggtgacctc cagcagcgcc tgcgccggat cgacgcgacc ggcggcgtat agcgcgggga
     7921 ggggcttctt gatggggggc atggcggtct tttgtgaggg ctccatcgta tccctgaggc
     7981 ggctttgtgc agatggcgcc cttgtaacga tcaggcggca aatccggcgg cgacgagtag
     8041 gaatttcccg gtaacggtct agggaatttg cttaagcccg aggccctcgc ggttccttcg
     8101 agcggttgag ctgggcagtg gcgcgcaagt ccgcatggcg aaagcggacg cgcgggtttc
     8161 accctagttc gtcagagcgc gttcagtttc gcgcaagccc cccgtcaggt gtcgaaacca
     8221 tcttgaaacc tatcagcttt ccgtaaggaa tcgccaacac agtcgcgcct tcaatgctgt
     8281 gagtgtcttt ccctttccag catgacacgt gcggcgattt attcgctgtg ttcttttcaa
     8341 ccgaggagat ttcatgaagg tagacaccaa cggtgaagcc tactggaagg ccacccttcg
     8401 actgctgacc cggattctga tcatctggtt tctagtgtcg ttcggcgcgg gcatcctgtt
     8461 cgccgacctg ctcaacaaca tcaagcttgg cggctatccg ctcggcttct ggttcgccca
     8521 ccagggctcc atctacgtct tcatcgcgtt gattttctat tacgccagga agatgggcca
     8581 gcttgaccgt cagttcgacg ttcacgaaga gtgaggaact gaaaatggaa ctccaaacca
     8641 ccatctacat cgtcgtcggc ctgagcttcg cgctctatat cggcatcgcc gtctgggccc
     8701 gtgccggctc gaccagcgag ttctacgccg ccggcggcag cgtccacccc gtgatgaacg
     8761 gcatggcgac cgcggccgac tggatgtcgg cggcatcctt catctccatg gccggcctga
     8821 tctccaacat ggg
//
LOCUS       DASWIY010000227         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3112332, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000227 DASWIY010000000
VERSION     DASWIY010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3112332"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1175)
                     /locus_tag="VGA59_08385"
     CDS             complement(111..1175)
                     /locus_tag="VGA59_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX9719735.1"
                     /translation="MAVAGLVAVAVVGTLCLTSCANLGYYWHTATGHLKVMSEARPLD
                     EWLGSSDAPDRLKERLALSQRIRDFAVAELKLPDNPSYRRYADLRRSAVVWNVVAAPV
                     YSVTLRNWCFPVTGCVTYRGYFDEGLARAEAAELAAQGYESNVYPVPAYSTLGWMNWA
                     GGDPLLNTFIHYPEGELARLLFHELAHQVVYARDDTMFNESFATAVERLGSARWLASR
                     ASEAARRQYAEFDGRRKQFRALALATRKRLEHIYETTTGEERDALKQATLAQFREQYA
                     QLRASWGGDPARYRGFDLWVERANNASFGAQAAYDELVPGFEALFEREGRDWKRFYDA
                     VKRLAEMPRGVRHQLLKDAA"
     gene            complement(1313..1648)
                     /locus_tag="VGA59_08390"
                     /pseudo
     CDS             complement(1313..1648)
                     /locus_tag="VGA59_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
     gene            1713..2843
                     /locus_tag="VGA59_08395"
     CDS             1713..2843
                     /locus_tag="VGA59_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9719736.1"
                     /translation="MRLIHSFARTLMVAALALLSAAGQAQDGLTKTSVVLGQSLALTG
                     PGSSLAVPFHQGAKVYFDRVNAAGGINGRKMELVTLDDRGNAQQAIENTRKLLEQGVF
                     SLFGYYGSPQVTAAYPQIKDGEILLFAPMAAADEFRGAMYGNVYSMRPGYSEEAAAIA
                     RHAETLGAKRLAILHANDSESLAALESATRTMSGMGANLVANAVVTSGSVASSVTKLL
                     AAKPESVLVIGDAGSAAGAVRELRAKGFRGPIYGFSNTGESLLAEQLGPAGAGVVVVR
                     VVPKSDSAKVAIVRELLADAQASKLGKPNVYMLEGYIAARVYAEALRRIPRDLNRGKF
                     RKALDGLDDVSVGGFRVHFADDRVASKLVELGLIDSQGRVRE"
     gene            complement(3068..>3250)
                     /locus_tag="VGA59_08400"
     CDS             complement(3068..>3250)
                     /locus_tag="VGA59_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9719737.1"
                     /translation="TTLVAFGTEAGIYKKAGIPTVVCGPGSIAQAHQPDEFVSLEQLA
                     RCEAFMRALAAVREIG"
BASE COUNT          542 a         1097 c         1071 g          540 t
ORIGIN      
        1 gccacctcgg cccaccggaa cgccagcttg cgcgcctcgg ccagtcccag gtgatgctgg
       61 cgtgtgatgt ggatgtcagc caactgggcc tcctggtttg gcgtggaaat tcatgcggcg
      121 tccttcagca gctggtgtcg cacccccctg ggcatctccg ccagccgttt gaccgcatca
      181 taaaaccgct tccagtcccg gccctcacgc tcgaacaacg cttcgaagcc gggcaccagt
      241 tcgtcgtagg cggcttgggc gccgaacgag gcattgttgg cacgctccac ccagaggtcg
      301 aagccgcgat agcgtgccgg atcgccaccc cagctcgcac gcagctgcgc gtactgctcc
      361 cgaaactgcg cgagcgtggc ctgcttcagc gcgtcgcgct cttcacccgt tgtcgtttca
      421 tagatgtgtt ccagccgctt gcgcgtcgcc agcgcgaggg cgcggaactg cttgcgccgg
      481 ccgtcgaact cggcgtactg ccggcgagcc gcctccgaag cccggctggc cagccagcgc
      541 gcgctgccca ggcgctcgac cgcggtcgcg aacgactcgt tgaacatcgt gtcgtccctg
      601 gcatagacca cctggtgcgc cagctcatgg aaaagcaacc gtgccagctc gccttcgggg
      661 tagtggatga aggtattgag cagcggatcg ccgccggccc agttcatcca gccgagcgtg
      721 gagtaggcgg gcaccgggta gacgttggac tcgtaaccct gcgccgccag ctccgccgcc
      781 tcggcgcggg ccaggccttc gtcgaagtaa ccgcggtagg tgacgcagcc ggtcaccgga
      841 aaacaccagt tcctgagggt gaccgaatac acgggagcgg ccacgacgtt ccagaccacg
      901 gcgctgcggc gcaggtccgc gtagcggcga tagctgggat tgtcgggaag cttcagctcg
      961 gccacggcga agtcgcggat gcgctggctg agcgccagcc gctccttgag ccgatcgggg
     1021 gcgtcgctgc tgcccagcca ctcgtccagc ggcctggcct cgctcatgac cttcaggtgg
     1081 cccgtagccg tatgccagta atagcccagg ttagcgcagc tggtcaggca cagcgtgccc
     1141 acgaccgcca ccgccaccag tcccgcaaca gccatcgcca gccccttcct cacgctgcgc
     1201 ggacttcgac caggcagtcg tagaacaccg ggccacgccc caggtccgtc agattctggc
     1261 tggtcagctg gttgacattg gtgccctgga gcccgagctt gcgccaccac acggcgatcg
     1321 cctccaggcg ccgcgcgatg tcgccgaggg cgtcgtccca gctcacgcgt tcgaagctgc
     1381 cggtgccctt cgggccgcgc cggcgcatcg ggtacagcag gcgctccgtg tggtaggtac
     1441 gctgcgcgta gcgcgagact ttcgtgcaga gagcgccctg ggtatgccgg tgttccggat
     1501 tgccgcggac ctcgatcgcc acgccgtccc gcacggtcgt gaccagcgcg caggtgtccg
     1561 ggcaatcgtg cgggcaggcg ccgcgcaccc gttctgaaga ctttgttgcg aaattgccgc
     1621 taggggagat gccgatggtg ctactcatga gctaacccta tagtttgacg gtcggccctg
     1681 tcatttcatt tccaggccgc aggagaacaa gcatgaggtt gatccattcg ttcgcacgca
     1741 ccctgatggt agccgccctc gcactgctca gtgcggccgg acaggcgcag gacggcctga
     1801 ccaagaccag cgtcgtgctg ggccagtcgc tggccctgac ggggccggga tcttcgctgg
     1861 ccgtgccgtt tcaccagggc gccaaggtgt atttcgaccg ggtgaacgcg gcgggcggca
     1921 tcaacggccg caagatggag ctggtgacgc tggacgaccg gggcaatgcc cagcaggcga
     1981 tcgagaacac ccggaaactg ctcgaacagg gcgtgttctc cctgttcggc tactacggct
     2041 cgccccaggt gactgccgcc tacccgcaga tcaaggatgg cgagatcctg ctgttcgcac
     2101 cgatggccgc ggccgacgag ttccggggcg ccatgtacgg caatgtgtat tcaatgcggc
     2161 cgggctattc cgaagaagcg gccgccatcg cgcgccatgc ggaaaccctg ggggccaaac
     2221 gcctggccat cctgcacgcc aacgacagcg aatcgctggc ggcgctcgaa tcggccacgc
     2281 ggaccatgtc gggcatgggc gccaacttgg tcgccaacgc agtggtgacc agcggcagcg
     2341 tcgccagcag cgtgaccaag ctcctcgccg cgaaacccga atcggtgctg gtcatcggcg
     2401 acgccggcag tgcggccggc gccgtgcgcg agctgcgcgc caagggcttt cgcggcccga
     2461 tctatggttt ttccaatacc ggcgagagcc tgctggccga gcagctgggc ccggccggcg
     2521 ccggcgtcgt cgtggtgcgc gtggtgccca agagcgattc cgccaaggtg gcgatcgtgc
     2581 gcgagctgct ggccgacgcc caggcctcga aactgggcaa accgaatgtc tacatgctgg
     2641 agggctacat cgccgcacgc gtgtacgctg aagcgctgcg ccgcatcccg cgcgacctca
     2701 accgcggcaa gttcaggaag gcgctggacg ggctcgacga tgtgagcgtc ggcggctttc
     2761 gcgtccactt cgccgatgac cgcgtcgcct ccaagctggt cgagctcggc ctgatcgact
     2821 cccagggccg ggtgcgcgag taaaggccaa gagtcggccg cgctgtcacc ccgggctcga
     2881 caaataggtt gtcgtcccgg gttgacaaat aggctgtcat ccggacttga caaataggct
     2941 gtcatcccgg gcttgacccg ggatccatgg attgcggatc gagtccgcaa tgacaaggca
     3001 aataggctgt cgtccggggc tcgacaaata ggctgtcatc ccggcaatca ggaccggccc
     3061 gcgcggatta cccgatctcg cgcacagcgg ccagagcgcg catgaaggcc tcgcaccgcg
     3121 ccagctgttc caggctcacg aattcgtcag gctggtgcgc ctgcgcgatg ctgccggggc
     3181 cgcatacgac ggtgggaatg ccggccttct tgtaaatccc ggcctcggtc ccgaaggcca
     3241 cgagcgtcgt
//
LOCUS       DASWIY010000228         8661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_518119, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000228 DASWIY010000000
VERSION     DASWIY010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518119"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..959)
                     /locus_tag="VGA59_08405"
     CDS             complement(207..959)
                     /locus_tag="VGA59_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9719738.1"
                     /translation="MENLIFIVILGATLAGFVQGLSGFGFGLTAMSLWAWTLEPRLAA
                     ALAVFGALTGQIIAAVAVRRGFNFQRLLPFVAGGLAGVPVGIYILPRLDVPLFKGLLG
                     LLLVVMCPLMFFAARLPRIAAGGRAGDALAGAAGGMMGALGGFTGVVPTLWCTLRGFE
                     KDTQRAVIQNFNLAMLTVTFISYMATGIATRQMLPLFAIVAPAMLIPSLLGARLYLGI
                     GETGFRKVVLTLLTASGVAMLVSALPVLARRW"
     gene            complement(1139..4096)
                     /gene="gcvP"
                     /locus_tag="VGA59_08410"
     CDS             complement(1139..4096)
                     /gene="gcvP"
                     /locus_tag="VGA59_08410"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX9719739.1"
                     /translation="MLMQSARPLGELENPAEFIARHIGVGPADERHMLSVIGESSRRA
                     LIDGIVPRSIVRARPMKLPEAVTESAALAELKAIADRNQVFRSFIGQGYHGTHTPGVI
                     LRNVLENPAWYTAYTPYQSEISQGRMEALVNFQTMVSDLTGMPIANASMLDEATAAAE
                     AMTLAKRSVRSKSNLFIVAGDCHPQTIEVIQTRARPLGIEVRVGCAPDLMKEHDYFGV
                     LAQYPATGGTVDDLRPLVERAHAKNAAFCVAADLLALTLLVPPGEWNADIVLGTTQRF
                     GMPMGAGGPHAAYLACRDEFKRSLPGRLVGVSIDTHGNPAYRLALQTREQHIRREKAT
                     SNICTAQVLPAVIASMYAVYHGPQGLKRIAARVAGYTAVLAKGLREMGYTLTSDSAFD
                     TITVQTGDATDLIASRAHSARANLRNTLPRHLGISLDETTTREDIQLLWSFFARPGQA
                     LPEIAKYVKGTEPLIPSELRRTSEFLTHPVFNTHHSETGMLRYIRALADKDLALDRSM
                     IPLGSCTMKLNATSEMIPITWPEFAQVHPFAPRDQLQGYAELDEQLRAWLCEATGYAG
                     VSLQPNAGSQGEYAGLLAIRAFHESKGQGHRNICLIPSSAHGTNPASAQMAGMRVVVT
                     ACDANGNVDLADLKAKCEQHSANLAAVMITYPSTHGVFEMQVKELCALVHRHGGRVYV
                     DGANMNALVGVAAPGEFGGDVSHLNLHKTFCIPHGGGGPGVGPVCVVEDLVPFLPGHA
                     AFEAAQLVRAEAVETVERAQRQPFHVGAVSAAPLGNAAVLPISWMYCRMMGADGLKQA
                     TEVAILSANYISARLRDHYPTLYASANGHVAHECILDLRPLKESSGVSAEDVAKRLID
                     YGFHAPTLSFPVAGTLMVEPTESETLDELDRFIGAMVAIRGEIRRIERGEWPQDDNPL
                     KHAPHTAASLLKGEWTHAYPRDVGAAVLDPGSGSGAGSIRHAKYWPPVGRVDNVYGDR
                     NLFCSCVPVSAYE"
     gene            complement(4236..4613)
                     /gene="gcvH"
                     /locus_tag="VGA59_08415"
     CDS             complement(4236..4613)
                     /gene="gcvH"
                     /locus_tag="VGA59_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX9719740.1"
                     /translation="MTVKYTEEHEWIQLEDHEAAVVGITLHAQDALGDVVFVDLPEIG
                     RSYAKGDVAGVVESVKAAADIYMPVAGEVTEVNEELRADPSLANRDPMGNGWFFKILI
                     KDMAEFDELMDEPAYARLLKNTA"
     gene            complement(4644..5849)
                     /gene="gcvT"
                     /locus_tag="VGA59_08420"
     CDS             complement(4644..5849)
                     /gene="gcvT"
                     /locus_tag="VGA59_08420"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX9719741.1"
                     /translation="MSEEQPLLHTPLHGLHLELGARMVPFAGYAMPVQYPAGLMAEHR
                     QTRNCAGLFDVSHMGQLRLAGPDAAAAFETLVPVDVIDLPVGKQRYGLLLTEQGTIID
                     DLMFVNCGDDPSFAGPSQGASAPSGGSEPSSERGGTIFVIVNGACKAGDIAHIRKHIG
                     SRCEVIAMPERALLALQGPKAVDALARLVPGVEKLVFMTGDRFDWRGAGLFITRSGYT
                     GEDGFEISVNEAQADALARALLAQPEVKPIGLGARNSLRLEAGLCLYGNDIDTTTTPV
                     EAALTWAIQKVRRSGGARAGGFPGAAKVLGQLQGGVPVERKRVGLVALERVPVREHTE
                     LQDAQGQRIGEVTSGLLAPTTDRPIAMAYVATALASPGTRVNAIVRGKPVVMEVATMP
                     FVPNGYHRG"
     regulatory      complement(5893..6001)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(6013..6102)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(6278..7555)
                     /locus_tag="VGA59_08425"
     CDS             complement(6278..7555)
                     /locus_tag="VGA59_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9719742.1"
                     /translation="MTAVMVGTMVLCFALTISVAVSIGLASILGIQASNANMLLSVKE
                     MFNAINKFPLAAIPFFILAGNLMETGGISRRLVEFAKSIVGGVQGGLPMTCVLTCMIF
                     AAVSGSSVATTFAIGAILIPALLKHGYPVTYAAALQATSAELGVVIPPSIPMILYGVS
                     AEVSIGELFIAGFGPGVLIGGALMLFAYFYCRWKGWGKNDGDGRLPVGQATWQAGWAL
                     AMPVIILGGIYGGIFTPTEASAVAVGYALLVGMIVYREISPFNLYVILRKSVISSAVI
                     MFIIANAGLFAFLITRAGVPEAIGRWLEEVLRSPVYFLLGINVALFVIGMFIETSAAI
                     IVLAPILVPVAQHFGVDPVHFGMVMVVNLALGMITPPFGVNLFAACTVARISLDRIVK
                     QLVPFVLVILACLMIVTYVPALSLTLRDLAYAK"
     gene            complement(7578..8072)
                     /locus_tag="VGA59_08430"
     CDS             complement(7578..8072)
                     /locus_tag="VGA59_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9719743.1"
                     /translation="MKQGFLNFERWATGFSMAVACTMMVIAASLGIFQIVTRFVLEQP
                     AEWSEILIRFSLIWMVFLGIPAAFRQGAMVSVDVLYRWSPPKFRRVLDWVVSIAALAL
                     IAIIVWYGWDYANRGSVQSMAGLESVSMFWAYLAMPVGGLFCVIGIIGNLLDPVRMEL
                     ETAQ"
     gene            complement(8165..>8661)
                     /gene="dctP"
                     /locus_tag="VGA59_08435"
     CDS             complement(8165..>8661)
                     /gene="dctP"
                     /locus_tag="VGA59_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9719744.1"
                     /translation="DLKGLKMRTMENPVHIAAYKGFGIVTTPMAFPEVFTALQQGTVD
                     GQENPLSVIISAKLDQVQKHLSLTGHVYSPAIFLMNKAAFDKLSPADKKAFVEAAKEG
                     TKANRARVDEDDAKGVAYLRSKGMTVIDNLDKSKFVAALAPVNTEFEKQFGKANLDKI
                     RNYK"
BASE COUNT         1488 a         2806 c         2858 g         1509 t
ORIGIN      
        1 gaacacctgg tcaagcacgc tctgcaggcg gttcagctcg gaaaagatgt ccgccgcagg
       61 tctgaaggtt gcgttcatca tcacagctct cctttgattc tgggattacg agcaaacaac
      121 tgattgcgtt gcgcagccgt cggccgcgcg aacctgaatt agcggctgcc cgccggtttt
      181 tcaagagcag cggcgaacgg cgcgcgctac cagcgccggg caagtaccgg cagcgccgag
      241 accagcatgg ccaccccgga ggcggtgagc aaagtcagga cgaccttgcg aaaaccggtt
      301 tcgccgatgc ccaggtagag ccgggcgccg agcagggaag ggatgagcat ggccggcgcg
      361 acgatggcga acagcggcag catctggcga gtggcgatgc cggtggccat gtagctgatg
      421 aaggtcaccg tcagcatcgc cagattgaag ttctggatca cggcgcgctg cgtgtccttt
      481 tcgaagccgc gcagagtgca ccaaagggtg ggcaccacgc ccgtgaatcc ccccagagcg
      541 cccatcatcc cgccggccgc gccggccaac gcgtcgccgg cccgcccgcc cgccgcgatg
      601 cgcggcaggc gcgccgcgaa aaacatcaaa gggcacatca cgaccagcaa aaggccgagc
      661 aggcctttga acaaggggac atccagtcgc ggcaggatgt agatgcccac cgggacaccg
      721 gcgagcccgc cggcaacgaa cggcagcagg cgctggaaat tgaagccgcg ccgcaccgcc
      781 acggccgcga tgatctgccc ggtgagcgcg ccgaacacgg ccagggccgc cgccagtcgc
      841 ggctccagcg tccaggccca gagcgacatt gccgtcaggc cgaatccgaa tcccgacagg
      901 ccctgcacga aaccggccag ggtggccccg aggatgacaa tgaaaatgag gttttccacg
      961 ggagcattgt catcccggac ttgatccggg atccatgttg gcgtgcccag atggattgcg
     1021 gggccgggcc ggagcgtgat gcctcggtac gtgcatagcg caggcccggc gttgggctcg
     1081 ctgcactgga cagctgcgcg ccccgggcgt tccgcgagcc cagcccgcaa tgacaggctc
     1141 actcgtaggc ggacaccggc acgcagctgc agaacaggtt gcggtcgccg tagacgttgt
     1201 cgacgcggcc taccggcggc cagtacttcg cgtgccggat ggagcctgcc ccggaccccg
     1261 atccggggtc gagcaccgcc gcgcctacat cgcgtggata cgcgtgcgtc cattcgccct
     1321 tgagcaggct ggccgccgta tgcggcgcgt gcttcagcgg gttgtcatcc tggggccatt
     1381 cgccgcgttc gatgcggcgg atctcgcccc ggatggcgac catcgcgccg atgaagcggt
     1441 ccagctcgtc cagcgtctcg ctttcggtcg gctccaccat cagcgtgccc gccacgggga
     1501 agctcagagt gggcgcatgg aagccatagt cgatcaggcg cttggccacg tcctcggcgc
     1561 tgaccccgct ggattccttc aggggccgca ggtccaggat gcactcgtgg gccacgtgcc
     1621 cgttggcgct tgcgtacagc gtcgggtagt ggtccctcag ccgtgcgctg atgtagttcg
     1681 cgctcaggat ggcgacttcg gtggcctgct tcaggccgtc cgcgcccatc atgcggcagt
     1741 acatccagct gatcggcagc acggccgcat tccccagcgg cgcggcggac accgcgccca
     1801 cgtggaacgg ttgccgctgg gcccgctcca cggtctcgac cgcttcggcc cggaccagtt
     1861 gcgccgcctc gaaagccgcg tggccgggca ggaacggcac gaggtcttcc accacgcaca
     1921 ccgggcccac gcccggtccg ccgccgccat gcgggatgca gaacgtcttg tgcaggttca
     1981 ggtggctcac gtcgccgccg aattcgcccg gcgcggccac gcccaccagt gcgttcatgt
     2041 tggcgccgtc gacgtacacg cgcccgccgt gccggtgtac cagggcgcac agctccttga
     2101 cctgcatctc gaacacgccg tgcgtgctgg ggtaggtgat catcacggcg gcaaggttgg
     2161 cgctgtgctg ctcgcacttg gccttcaggt ccgccaggtc cacgttgcca ttggcatcgc
     2221 acgccgtcac gaccacccgc attccggcca tctgcgcgct cgcgggattg gtcccgtgcg
     2281 ccgacgaagg gatcaggcag atgttgcgat ggccctggcc cttgctctcg tggaacgccc
     2341 tgatcgccag caggcccgcg tactcgccct gggagcccgc gttgggctgc aggctgacgc
     2401 cggcgtaacc cgtggcctcg cacagccagg cgcgcagctg ttcgtcgagt tccgcatagc
     2461 cctgcagctg gtcgcgtggc gcgaacggat gcacctgagc gaattccggc cacgtgatgg
     2521 ggatcatttc gctcgtggca ttgagcttca tcgtgcagct gcccagcggg atcatgctgc
     2581 ggtccagcgc caggtccttg tcggccagtg cccggatgta gcgcagcatg ccggtttcgg
     2641 agtggtgcgt gttgaacacc gggtgcgtga ggaactcgct ggtgcggcgc agctcggacg
     2701 gaatcagcgg ctcggtgccc tttacgtatt tcgcgatttc gggcagggcc tggcccggcc
     2761 tggcaaagaa ggaccacagc aactggatgt cttcgcgcgt ggtggtttcg tccagcgaaa
     2821 tgccgaggtg gcggggcagg gtgttgcgca ggttggcccg ggccgaatgg gcgcggctgg
     2881 cgatcaggtc ggtggcgtcg ccggtttgga cggtgatcgt gtcgaatgcg ctgtcggacg
     2941 tgagcgtgta gcccatctcc cgcagcccct tggccagcac ggcggtgtag ccggccacgc
     3001 gcgcggcaat ccgcttgagc ccctgcgggc cgtggtagac cgcgtacatg ctggcaatca
     3061 cggccggcag cacctgcgcg gtgcagatgt tcgaggtggc tttctcgcgg cggatgtgct
     3121 gctcgcgcgt ctgcaaggcc aggcggtagg ccgggttgcc gtgcgtgtcg atgctgacgc
     3181 ccaccaggcg gcccggcaat gagcgcttga attcatcgcg gcacgccagg tacgccgcgt
     3241 gcggcccgcc ggcgcccatg ggcatgccga agcgctgcgt ggtgcccagc acgatgtcgg
     3301 cattccattc gccgggcggc accagcaagg tgagcgcgag cagatcggcg gcgacgcaaa
     3361 aggcggcgtt cttcgcgtgc gcgcgttcca ccagcggccg caggtcgtcg accgtgccgc
     3421 cggtggcggg atattgcgcc agcacgccga aatagtcgtg ttccttcatg aggtcgggcg
     3481 cgcagcccac gcgcacttcg atgcccagcg gcctggcccg tgtctggatc acctcgatcg
     3541 tctgcggatg gcagtcaccg gccacgatga acaggttgct cttgctcctg acgctgcgct
     3601 tggccagtgt catcgcttcg gccgccgcgg tcgcttcgtc cagcatcgac gcgttggcga
     3661 tgggcatgcc cgtcaggtcg ctgaccatgg tctggaaatt caccagcgct tccatgcggc
     3721 cctggctgat ctccgactgg tagggcgtgt atgcggtgta ccaggccggg ttctccagca
     3781 cgttgcgcag gatgacgccg ggtgtgtgcg tgccgtggta gccctggccg atgaaactcc
     3841 tgaagacctg gttgcggtcc gcgatcgcct tgagctcggc cagagccgcc gactcggtga
     3901 ccgcctccgg cagcttcatc gggcgggcgc ggacgatgga gcgcggcacg atgccgtcga
     3961 tgagggcgcg ccgcgaggac tcgccgatga cggatagcat gtgacgttcg tcggctggcc
     4021 cgacaccgat atgacgggcg atgaattcgg cagggttctc gagctcgccg agcgggcggg
     4081 cggattgcat caacatgggg gctccagtgg gatgtcgtcc cggacttgct gtcatcccgg
     4141 actcgatccg ggatccatgg attgcgggtg ctgaaacccc ggacttgatc cggggccgca
     4201 atgacagggg gtctggcgtt tttcggcggg aaacgtcagg cggtgttctt cagcaaacgc
     4261 gcgtatgccg gctcgtccat gagctcgtcg aactcggcca tgtccttgat gaggatcttg
     4321 aagaaccagc cgtttcccat cgggtcccgg ttggccagcg acggatcggc gcgcagttcc
     4381 tcgttgacct cggtgacctc tcccgccacg ggcatgtaga tgtcggccgc cgccttgacc
     4441 gactcgacca cgcccgccac gtcgcccttg gcgtaggaac ggccgatctc cggcaagtcg
     4501 acgaacacca cgtcgcccag cgcgtcctgc gcgtgcaagg tgatgcccac gacggcggcc
     4561 tcgtggtcct cgagctgaat ccattcatgc tcctcggtgt atttcacggt catgcgtttc
     4621 tccttgaaaa gggtctcgat tgtctagcca cggtggtagc cgttcggcac gaagggcatt
     4681 gtggcaactt ccataaccac cggcttgccg cgcacgatcg cgttcacgcg tgtgcccggc
     4741 gaggccaaag ccgtggcgac gtaagccatc gcgatgggcc tgtcggtggt gggggccagc
     4801 agcccgctgg tcacctcgcc gatgcgctgt ccctgcgcat cctgcagttc ggtgtgctcg
     4861 cgcaccggga cgcgttccag cgccaccagg ccgacccgtt tgcgttccac ggggacgccg
     4921 ccctgcaact ggcccagcac tttggccgcg cccggaaagc cgcccgcgcg ggcgccgccg
     4981 ctgcgccgca ctttctggat cgcccaggtc agcgcggctt cgaccggcgt ggtcgtggta
     5041 tcgatgtcgt tgccgtagag gcacaggccc gcttccagcc gcagcgagtt gcgcgctccc
     5101 aggccgatgg gcttgacctc gggttgggcg agaagggcgc gggccagtgc atcggcctgc
     5161 gcctcattta ccgagatctc gaatccgtcc tcgccggtgt agccgctgcg cgtgatgaac
     5221 agaccggccc cgcgccaatc gaagcggtcg ccggtcatga acacgagctt ctccacgccc
     5281 ggcaccagcc gcgccaatgc gtcgaccgcc ttcggtccct gcagggcgag cagcgcgcgc
     5341 tcgggcatgg ctatcacctc gcagcgagag ccgatgtgct ttcgtatgtg ggcgatgtcg
     5401 cctgccttgc acgcgccgtt gacgatcacg aatatcgtgc ccccacgctc gcttgaaggc
     5461 tcgctgcccc ccgagggggc gctcgctcct tgggacggcc cggcgaagct cggatcatcc
     5521 ccgcaattga cgaacatcag gtcgtcgatg atcgtgccct gttcggtcaa cagcaatcca
     5581 tagcgctgct tgcccacggg caggtcgatc acgtcgaccg gcaccagcgt ttcgaaggcc
     5641 gcggccgcat cgggacctgc caggcgcagt tgccccatgt gcgagacatc gaagagtccc
     5701 gcgcaattgc gcgtctggcg atgctcggcc atgaggccgg cggggtactg gaccggcatc
     5761 gcgtagccgg cgaagggaac catgcgggcg cccagttcca ggtgcagccc atgaaggggg
     5821 gtgtggagca ggggttgttc ttcggacaag tggctctcca ggggcaaacg tcaaccatgg
     5881 tacacaccat ggacggcccc tgctgtccgc tttacctgag agattcaccc cgttacgggg
     5941 tttgctcctt cggtgggccg catgcctggt cggcttgcgg cctctctcca gcaaggcacg
     6001 ccccgcaacc ggggcgtttc gtcagtcctt tttgcctgag cgttcggatt ggtcctgcgc
     6061 cttcggcggt tccgcgtagg aactctctcc tgaccgttgg attgtacctg ccccccatgg
     6121 attgcggatc gagtccgcaa tgacaagggc ggtaccggga tgactcggag gtttgtcatc
     6181 ccgggcttga cccgggatcc atggattgcg gatcgagtcc gcaatgacaa gacggcgccg
     6241 ggatgactcg gctcgcatgc ttgcatgctt cgccgagtca tttcgcgtat gcgaggtcgc
     6301 gcagcgtcag cgacaacgcc gggacataag tgacgatcat caggcaggcc aatatcacga
     6361 gaacgaatgg aaccagctgc ttgacgattc gatcgagcga gatgcgggcc acggtgcagg
     6421 ccgcgaacag gttgacgccg aacggcggtg tgatcatgcc cagcgcaagg ttcaccacca
     6481 tgaccatccc gaaatgcacg gggtcgacgc cgaaatgctg ggccacgggc accaggatgg
     6541 gtgccagcac gatgatcgcc gcactggttt cgatgaacat cccgatgacg aacagtgcga
     6601 cgttgatccc caacaggaag tagacgggac tgcgcagcac ttcttccagc cagcgcccga
     6661 tcgcttcggg cactccggcg cgcgtaatga ggaatgcgaa caggcccgcg ttggcgatga
     6721 tgaacatgat caccgccgat gaaatgacgg acttgcgcag gatgacatag agattgaacg
     6781 ggctgatttc ccggtagacg atcatcccga ccagcagcgc gtagccgacc gccaccgcgg
     6841 aagcctcggt gggcgtgaag atgccgccgt agatgccgcc caggatgatg accggcatcg
     6901 cgagcgccca acccgcctgc caggtagcct ggccgacggg cagccggcca tcgccgtcgt
     6961 tcttgcccca gcccttccac ctgcaataaa aataggcgaa cagcatcagc gcgccgccga
     7021 tcaggacgcc cggcccgaag ccggcaatga acagctcgcc gatcgacact tcggcgctca
     7081 cgccgtacag gatcatcgga atggagggcg ggatcacgac gcccagttcg gcgctcgtgg
     7141 cttgcagggc agccgcgtag gttaccgggt agccgtgctt gagcagagcc gggatcagga
     7201 tcgcgccgat cgcgaaagtg gtcgccaccg acgaacctga aacggctgcg aaaatcatgc
     7261 aagtgagcac gcaggtcatg ggcagcccgc cctgcacccc gccgacgatg ctcttggcga
     7321 attcgacaag ccggcgcgat atgccgccgg tctccatcag gttgcccgcc aggatgaaga
     7381 acggaatcgc cgccagcggg aacttgttga tggcgttgaa catctccttg acggaaagca
     7441 gcatgttggc attcgacgcc tggatgccca ggatcgacgc gaggccgatc gaaacggcaa
     7501 ccgaaatggt gagtgcgaag cacagcacca tggtgccgac catgacggcg gtcatgcgaa
     7561 ctctcccgag gctgcgctca ttgcgcggtc tccagttcca tccggaccgg gtccagcagg
     7621 ttgccgatga tgccgatcac gcagaagagc ccaccgacag gcatggcgag gtaagcccag
     7681 aacatcgaga cgctttcgag tccggccatg gactgcacgc tgccgcgatt cgcgtagtcc
     7741 cacccgtacc agacgatgat ggcgatgagc gccagcgcgg cgatggatac cacccagtcg
     7801 agcacgcgac ggaatttggg cgggctccag cggtagagca cgtccacgct gaccatcgcg
     7861 ccctgccgga atgcggccgg gatgcccagg aacaccatcc agatcaggct gaagcggatc
     7921 aggatctccg accattctgc gggctgctcc aacacaaagc gtgtgacgat ctggaatatg
     7981 ccgaggctgg cggcgatcac catcatggtg cacgccactg ccatcgaaaa cccggtggcc
     8041 caccgctcga aattcaggaa gccctgtttc atgtttcgcg caacccggtg aggctggaaa
     8101 atccaaagaa aacgcccgaa agccggtgga ctcgcgggcg cctcatgcta tccagggata
     8161 gcgatcactt gtaattgcgg atcttgtcga gattggcctt gccgaactgc ttctcgaact
     8221 cggtgttcac cggcgccagc gccgcgacga acttggactt gtcgaggttg tcgatcaccg
     8281 tcatgccctt gctgcgcagg taggcgacac ccttcgcgtc atcctcgtcc acgcgcgcgc
     8341 gattggcctt ggttccttct ttcgccgctt ccacgaaagc ctttttgtcg gcagggctga
     8401 gcttgtcgaa cgccgccttg ttcatcagga agatagcggg cgagtacaca tggccggtca
     8461 gggacaggtg cttttgcacc tggtcgagct tggccgaaat gatcacggac agcgggtttt
     8521 cctggccgtc aacggtgccc tgctgcaggg cggtgaacac ttcggggaaa gccatcgggg
     8581 tggtcacgat gccgaatccc ttataggcgg cgatatgcac cggattttcc atggtgcgca
     8641 tcttcaggcc cttgaggtct c
//
LOCUS       DASWIY010000229         3218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_518320, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000229 DASWIY010000000
VERSION     DASWIY010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518320"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VGA59_08440"
     CDS             complement(<1..645)
                     /locus_tag="VGA59_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9719745.1"
                     /translation="MKTVPHSPLEPSAASKWFAGIVVIAYAVITLIPLAWIVMTSFRT
                     PADSIAYPPKVVAEASLVGYVNLFTTRSRVSADELASLPPAKTWYEEVVREQQMILAG
                     PSRFSDRYFNSLAIGFGSTLLSVVLGTLAAYAFSRFRVPGKDDWLFFILSTRMMPPIA
                     VAIPIYLMFRNLGLNDTHLGLILLYTVVNLSLAVWLLKGFMDEIPREYEEAAMVD"
     gene            complement(642..1652)
                     /locus_tag="VGA59_08445"
     CDS             complement(642..1652)
                     /locus_tag="VGA59_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9719746.1"
                     /translation="MKPRVFMQWPQQPQAVATANPSEPAASSRPASARTLAPSTLLAR
                     QGLSDRALAWLFVGPTIALLLAFNIFPLLWTIWLSFTNFRANRPNAEVVWLGIDNYRR
                     VLGNEAIWENMRATAHFLVSSIALQLVLGFALALLLNRRFRSHSFWSTAILLPMMLAP
                     AVVGTFWKYFFEPQYGIFNAIARFLGGSGDFTMLGSTQLSPWAIVLVDTWMWTPYVML
                     LLLAGLKSIPPYLYEAAEIDRASEWTKFWRITLPMVMPFILLALLFRTIENFKMFDLV
                     DQLTNGGPGSVTELASIHLKREAFEKWRTGYASAFAIILFVAIYGLSLVTVRFLDKVK
                     QR"
     gene            complement(1675..3006)
                     /locus_tag="VGA59_08450"
     CDS             complement(1675..3006)
                     /locus_tag="VGA59_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9719747.1"
                     /translation="MSKPKSTLWRLVAGAALGLSALGAQAQDKQLTLCWAAWDPANAL
                     VELSKDFTAKTGIQMKYEFVPWPNFAQRMLNELNSRGKLCDLLIGDSQWIGSAATEGH
                     YVKLNDFFDKYAIKMSDFMPATVEGYSTWPKGTKNYWALPAMGDAVAFTYRKDWFARP
                     ALQAEFKAKHGRDLAPPKTWDEFKQVAQFFQSRNIDGKKVYGAALYTERGSEGITMGV
                     TNTLYAYGFDYANPAKPYDMKGYVNGPGAVKGLKFYKELYDCCVPPGHSNAYMTEDLD
                     AYKSGQVAMQMNFIAFFPGISKDPQVGGEKTGFFPIPKGDKQFAQLGGQGISVVQYSD
                     KKDMALQYMKWFAQPAIQKKWWDGGGFTCLKSILESPGFETSTPYAKVFLDSMKIVKD
                     FWADPSYAQLMLAMQNRVHDYVVAGKGTPNAALDALLKDWTEVFKEDGKLK"
BASE COUNT          586 a          988 c         1056 g          588 t
ORIGIN      
        1 gtccaccatc gccgcttcct cgtactcgcg cgggatctcg tccatgaagc ccttgagcag
       61 ccagaccgcg agcgacaggt tcaccaccgt gtacagcagg atcagcccca ggtgcgtgtc
      121 gttcagcccg aggttgcgga acatcaggta gatcgggatc gccaccgcga tgggcggcat
      181 catccgcgtg gacaggatga agaacagcca gtcgtccttg cccggcaccc ggaagcgcga
      241 gaaggcgtag gcggccaggg tccccagcac caccgacagc agcgtcgagc cgaagccgat
      301 cgccagcgag ttgaagtagc ggtcggaaaa gcgcgaggga ccggccagga tcatctgctg
      361 ctcgcgcact acctcctcgt accaggtctt ggccggcggc aacgaagcca gttcgtcggc
      421 cgagacccgc gagcgcgtgg tgaacaggtt gacatagccg accagcgagg cctcggccac
      481 caccttgggc gggtacgcaa tggaatcggc cggcgtgcga aagctcgtca tgacgatcca
      541 ggccagcgga atcagcgtga tgacggcgta ggcgatcacc acgatgccgg cgaaccactt
      601 ggacgcggcg ctgggctcca gcgggctgtg agggacggtt ttcatctctg cttcaccttg
      661 tcgaggaacc gcaccgttac cagggacagg ccgtagatgg cgacgaacag gatgatggcg
      721 aaggccgatg cgtagccggt gcgccatttc tcgaaggcct cgcgcttgag gtggatcgac
      781 gccagctcgg tcaccgaccc gggcccgcca ttggtcaatt ggtccaccag gtcgaacatc
      841 ttgaagttct cgatggtgcg aaacagcagc gccagcagga tgaagggcat gaccatgggc
      901 agcgtgatgc gccagaactt ggtccactcc gaggcacggt cgatctcggc cgcctcgtac
      961 aggtaagggg gaatcgactt cagcccggcc agcagcagca gcatgacgta gggcgtccac
     1021 atccaggtgt ccaccagcac gatggcccag ggcgagagct gcgtgctgcc gagcatggtg
     1081 aagtcgccgc tgcccccgag gaaacgggcg atcgcgttga agatgccgta ctgcggttcg
     1141 aagaagtact tccagaaggt gcccaccacg gccggcgcca gcatcatcgg caacaggatg
     1201 gcggtgctcc agaagctgtg cgagcggaag cgccggttga ggagcagcgc caacgcgaaa
     1261 cccagcacca attgcagcgc gatcgaggac accaggaagt gcgccgtagc ccgcatgttc
     1321 tcccagatcg cctcgttgcc caggacgcgc cggtagttgt cgatccccag ccacacgact
     1381 tcggcgttgg gccggttcgc ccggaaattg gtgaacgaca gccagatcgt ccacagcagc
     1441 gggaagatgt tgaaggccag cagcagcgcg atggtggggc cgacaaagag ccaggccagg
     1501 gcccggtccg aaaggccctg gcgggccagc aaggtgctgg gcgccagggt gcgggcggaa
     1561 gccgggcggc tgctcgccgc cggctccgaa ggatttgcgg tggccaccgc ttggggctgc
     1621 tgcggccact gcatgaacac ccgaggtttc acgtgagctc ctgttgcctg gtgcttactt
     1681 gagcttgccg tcttccttga agacttcggt ccagtccttc agcagcgcgt ccagcgcggc
     1741 attgggcgtg cccttgccgg cgaccacgta gtcatgcacg cggttctgca tggccagcat
     1801 cagttgcgcg tagctcgggt cggcccagaa gtccttgacg atcttcatgg agtccaggaa
     1861 caccttggca tacggcgtgc tggtttcgaa gccgggagac tcgaggatgg acttcaggca
     1921 ggtgaagccg ccgccgtccc accatttctt ctggatggcc ggctgggcga accacttcat
     1981 gtactgcagc gccatgtcct tcttgtcgga gtactgcacc accgagatgc cctggccgcc
     2041 gagctgcgcg aactgcttgt cgcccttggg gatcgggaag aagccggtct tctcgccgcc
     2101 gacctgcggg tccttggaaa taccggggaa gaaggcgatg aagttcatct gcatggccac
     2161 ctggcccgac ttgtaggcgt ccaggtcctc ggtcatgtag gcgttgctgt ggccgggcgg
     2221 cacgcagcag tcgtacagtt ccttgtagaa cttcagaccc ttcacggcgc cggggccgtt
     2281 gacgtagccc ttcatgtcgt acggcttggc ggggttggcg tagtcgaagc cgtaggcgta
     2341 gagggtgttg gtcacaccca tggtgatgcc ttcggagccg cgctcggtgt acagcgccgc
     2401 gccgtagacc ttcttgccgt cgatgttgcg gctctggaag aactgggcca cctgcttgaa
     2461 ctcgtcccag gtcttgggcg gcgccaggtc gcggccgtgc ttggccttga actcggcctg
     2521 cagcgcgggg cgggcgaacc agtccttgcg gtaggtgaag gcgacggcgt cgcccatggc
     2581 cggcagcgcc cagtagttct tcgtgccctt gggccaggtc gaatagcctt cgaccgtggc
     2641 gggcatgaag tcgctcatct tgatcgcgta cttgtcgaag aagtcgttga gcttgacgta
     2701 gtggccttca gtggcggccg agccgatcca ttggctgtcg ccgatcagca ggtcgcacag
     2761 cttgcccctg gagttcagct cgttgagcat gcgctgcgcg aagttcggcc agggcacgaa
     2821 ctcgtatttc atctggatgc cggtcttggc agtgaagtcc ttcgacagct cgaccagcgc
     2881 gttggccgga tcccaggcgg cccagcacag cgtcaactgc ttgtcctgcg cttgcgcgcc
     2941 cagcgctgac agtcccagcg cggccccggc cacgaggcgc cagagggttg atttgggttt
     3001 gctcatgtgt cgtctccata cagtgcgtgg caggccacgc ttgggttgca aataaaaaag
     3061 cggcgtttcg ggtgctcaaa ccgcctgtct tctcttcggc tcaccgccga tcagcccact
     3121 gcctgcggcc ccgctgcttg aacaactcgc gccaggtcga ccgacttcgt ctgatgtgat
     3181 tgatggtatt cacatcattt aatacatcaa tcaagaca
//
LOCUS       DASWIY010000230         4914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_876846, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000230 DASWIY010000000
VERSION     DASWIY010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876846"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VGA59_08455"
     CDS             complement(<1..174)
                     /locus_tag="VGA59_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HEX9719748.1"
                     /translation="MSDTTFDYIIIGAGTAGCLLANRLSADSSKRVLLIEAGQKDDYY
                     WIHIPVGYLYCIGN"
     gene            complement(200..1741)
                     /locus_tag="VGA59_08460"
     CDS             complement(200..1741)
                     /locus_tag="VGA59_08460"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX9719749.1"
                     /translation="MNAPDKSRPNAAIASIEHWIGGRVVPGQSGRRADVFNPASGSVT
                     GQVALANSAEVAAAVAAAQAAFPAWADTPPIRRARVMFKFLELLNRHRDELAHAITAE
                     HGKVFTDAQGEVTRGIDIVEFACGIPQLLKGQYTDQVSTGIDNWTMRQPLGVVAGITP
                     FNFPVMVPMWMYPVAIAAGNSFVLKPSPLDPSASLMMAELLKQAGLPDGVFNVVQGDK
                     EAVDALLEHPDVKAISFVGSTAIAQKIYETGARHGKRVQALGGAKNHMVVMPDADMNQ
                     AVDALIGAAFGSAGERCMAISLGVLVGEAADKIMPMLAERTKALKIKNGTELDAEMGP
                     IVSSAAQGRIAGYIGQGEGEGAKLVVDGRKFQAAKAGAGCDNGFWIGGTLFDNVTPDM
                     KIYKEEIFGPVLGCVRVKDLAAAVDLINSHEYGNGVACFTSDGNVAREFSRRVQVGMV
                     GINVPIPVPMAWHGFGGWKKSLFGDMHAYGEEGVRFYTKQKSVMQRWPESIGKGAEFV
                     MPTAK"
     gene            1866..2804
                     /locus_tag="VGA59_08465"
     CDS             1866..2804
                     /locus_tag="VGA59_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9719750.1"
                     /translation="MTASAAPVLSDLHLLTVLAQTRSYTQAARRLGISKASVSMRIAE
                     LERATGVPLVRRTTRSVGLTEAGQQLVDDTQGAFTRIEQSFASIKDLAGTPRGLVRVT
                     APLALGRQHVAPTLAAFFRGYPEIRLELDLTDRLVNLAQEGFDLAIRHAHAPPDTHVA
                     WVLCPSRSLLVASGDYLRRHGAPTHPTDLASHQCLLYLRGTAGQSWAFERDGGRKAAE
                     KVVVAVNGPLRANNSEVLREAVLGGVGIGMLPDFTAAAHLGPRQLVRVLPQWQPRGFF
                     GERLYAIRPWSPQVPRAVQCFVDHLRQIFAQGFATD"
     gene            complement(2801..4597)
                     /locus_tag="VGA59_08470"
     CDS             complement(2801..4597)
                     /locus_tag="VGA59_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX9719751.1"
                     /translation="MNDKAVRIGGASGFWGDSGVGAPQLVRLGEIDYLVFDYLAELTM
                     SILAAARAKDPALGYATDFVTVTMKAVLKEVAARGIRVISNAGGVNPRACAGALAALA
                     AEQGVQVRIAVVQGDDVMPLVPELRAQGVTELQSGAGLPDRLLTANAYLGALPIKQAL
                     DDGGQIVVTGRCVDSAVTLGALMHEFGWPADDFDRLAQGSLAGHIIECGCQATGGLHT
                     DWEAVPDWAHIGYPVLECNADGSFIVTKPPGTGGLVNTAVIGEQMLYEIGDPRRYLLP
                     DVSCDFSQVTLEQAGEHRVRVQGARGLAPGPAYKVSATYMDGFRCSAQLTIVGQDAAR
                     KARRTGEAILERTRGLFTQHGLADYSRTNIEVLGSEEGNYGPHARTRDVREAVLRVAV
                     MHPQKAALELFAREIAPAGTSWAPGTTGADGRPRPSPAVKQFAFLLDKRRVSPEVVIG
                     DQVSPVAVPAGQSAVPLPVRPEVSTALSSGLKVGSGDEMAEVPLIRLAWGRSGDKGDS
                     SNIGVLARRPEWLPLLREQLTEARVAQWLAHLVQGSVTRYDVPGIHALNFLCTRALDG
                     GGMASLRNDPLGKGMAQILLSMPVRVPASWSL"
     gene            complement(4617..>4914)
                     /locus_tag="VGA59_08475"
     CDS             complement(4617..>4914)
                     /locus_tag="VGA59_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9719752.1"
                     /translation="LCLTGEPLTAAQALEYGLVNYVADDLDAKLDWLLQRLLDKSPAA
                     IRRGLYTMKKIEAMPFEESVAFTESQIALFTLTEDAKEGQQAFQQKRKPQWKGR"
BASE COUNT          734 a         1700 c         1634 g          846 t
ORIGIN      
        1 gttgccgatg cagtacaggt agcccaccgg aatgtggatc cagtagtagt cgtccttctg
       61 cccggcctcg atcaggagca cccgcttgga cgaatcggcg gacagccggt tcgccagcag
      121 gcacccggcg gttccggcac cgatgatgat gtagtcaaac gtggtgtcac tcattgccag
      181 aggccgcttc ggggggagct catttcgcgg tcggcatcac gaattccgcc cccttgccga
      241 tgctttccgg ccagcgctgc atgacggatt tctgcttggt gtagaagcgc acaccctctt
      301 cgccgtaggc gtgcatgtcg ccgaacaggc tcttcttcca gccgccgaaa ccgtgccagg
      361 ccatcggtac cgggatcggc acgttgatgc cgaccatgcc gacctgcacg cggcggctga
      421 attcgcgcgc cacgttgccg tcgctcgtga agcacgcgac gccgttgccg tattcgtggg
      481 agttgatcag gtccaccgcg gcggccaggt ccttgacgcg cacgcagccc agcaccgggc
      541 cgaagatttc ctccttgtag atcttcatgt ccggcgtgac gttgtcgaac agcgtgccgc
      601 cgatccagaa accgttgtcg cagccagccc cagccttggc ggcctggaac ttgcgcccat
      661 ccaccaccag cttggcgcct tcgccctcgc cctggccgat atagccggcg atgcggcctt
      721 gcgccgcgct gctcacgatg ggtcccattt cggcgtccag ctcggtgccg ttcttgatct
      781 tcaaggcttt ggtgcgctcg gccagcatcg gcatgatctt gtcggcggcc tcgcccacca
      841 gcacaccgag cgaaatcgcc atgcagcgct cgccggcgga accgaaggcc gcgccgatca
      901 gcgcgtccac ggcctggttc atgtcggcat cgggcatcac caccatgtgg ttcttggcgc
      961 cgcccagggc ctgcacccgc ttgccgtgcc gggcgccggt ttcatagatc ttctgcgcga
     1021 tggcggtgga gccgacgaac gagatggcct tcacgtcggg gtgctccagc agcgcatcca
     1081 cggcttcctt gtcgccctgg accacgttga acacgccgtc cggcaggccc gcctgtttca
     1141 gcaactcggc catcatgagc gaagccgacg ggtccagcgg gctgggcttg agaacgaagc
     1201 tgttgcccgc cgcgatggcc accgggtaca tccacatcgg caccatgacc gggaaattga
     1261 agggtgtgat tccggccacg acccccaacg gctggcgcat ggtccagttg tcgatgccgg
     1321 tggagacctg gtcggtgtac tgtcccttga gcagttgcgg gatgccgcag gcgaattcga
     1381 cgatgtcgat gccgcgcgtc acctcgccct gcgcgtccgt gaacaccttg ccgtgttcgg
     1441 cggtgatggc gtgcgcgagt tcgtcgcggt gccggttcag cagctcgagg aacttgaaca
     1501 tcacacgggc gcgccggatc ggcggcgtgt cggcccaggc cgggaaggcg gcctgggcgg
     1561 cggcgacagc ggcagcgacc tcggcgctgt tggccagcgc gacctggccg gtcacgctgc
     1621 cgctggccgg attgaagaca tcggcgcggc ggccgctctg gccggggacc acacgcccgc
     1681 cgatccagtg ttcgatggag gcgatcgcgg cgttcgggcg ggatttatcg ggggcattca
     1741 tggggacggc tttcaggatg gtcacatgca gtgggaaaag acgcccagtc tatgcttcaa
     1801 gccggccttt gagaatagcc agttattgac tacattgttc aaatgtttga acagctgata
     1861 ccgtcatgac cgcctccgcc gcgcccgtgc tttcggattt gcacctgttg acggtgctgg
     1921 cccagacccg cagctacacc caggccgcgc ggcgtctcgg tatctcgaag gcctcggtca
     1981 gcatgcgcat cgccgagctc gagcgcgcga ccggcgtgcc gctggtgcgg cgtacgacgc
     2041 gatccgtcgg cctgaccgaa gccggccagc aactggtgga cgacacgcaa ggcgcgttca
     2101 cgcgcatcga gcaaagcttc gccagcatca aggacctggc cggcacgccg cgcgggctgg
     2161 tgcgcgtcac cgcgccgctg gcactggggc gccagcatgt cgcgcctacc ttggccgcat
     2221 tttttcgcgg gtatcccgag atccggctcg agctcgacct gacggaccgg ctcgtcaacc
     2281 tggcgcagga gggtttcgac ctcgccatcc ggcacgccca cgcgccgccg gatacccacg
     2341 tggcctgggt gctttgcccg agccgttcgc tgctggtcgc gagcggcgac tacctgcgcc
     2401 gccacggcgc gcccacccat ccgaccgacc tggcgtcgca ccaatgcctg ctgtacctgc
     2461 gtggcacggc tggacaaagc tgggccttcg agcgcgacgg ggggcgcaag gccgcggaga
     2521 aagtggtcgt tgcggtcaac gggccgctgc gcgccaacaa cagcgaagtg ctgcgcgaag
     2581 ccgtgctggg tggcgtgggc atcggcatgc tgcccgactt cacggcggcg gcgcacctgg
     2641 gcccgcgcca gctggttcgc gtgctgccgc agtggcagcc gcgcggcttt ttcggcgaac
     2701 gcctgtacgc gatacggccg tggtcgccgc aggtgccgcg cgcggtgcag tgcttcgtcg
     2761 atcacctgcg ccagatcttt gcgcaggggt tcgcgaccga ctaaagactc cagctcgcgg
     2821 gaacccggac cggcatcgac agcaggatct gcgccatgcc ttttcccagg gggtcgttgc
     2881 gcagcgaagc catgccgccg ccgtcgagcg cgcgcgtgca caggaagttc agggcgtgga
     2941 tgccgggcac gtcgtagcgc gtgaccgacc cctgcaccag gtgcgcgagc cactgggcca
     3001 cacgggcttc ggtgagttgt tcgcgcagca gcggcagcca ttcgggacgg cgcgcgagga
     3061 ccccgatgtt cgagctgtcg cccttgtcgc cgctgcggcc ccaggccagg cggatcagcg
     3121 gcacctcggc catttcgtcg ccggaaccca ctttcaggcc ggatgagagc gccgtcgaaa
     3181 cctcggggcg aaccggcagg ggcacggcac tttggccggc gggcacggcg accgggctga
     3241 cctgatcgcc gatcaccacc tcgggcgaga cacgtcgctt gtccaggaga aacgcgaact
     3301 gcttcaccgc cggcgatggg cgcggccgtc cgtccgctcc ggtggtgccg ggcgcccaag
     3361 acgtgccggc cggcgcgatc tcgcgggcga acagctccag ggcggctttc tgcggatgca
     3421 tcaccgccac ccgcagcacg gcttcgcgca cgtcgcgcgt gcgggcgtgc ggcccatagt
     3481 ttccctcctc ggacccaagc acctcgatgt tggtgcggct gtaatcggcc aggccatgct
     3541 gcgtgaacaa accgcgcgtg cgctcgagga tcgcctcgcc ggtgcgcctg gccttgcgcg
     3601 cggcgtcctg cccgacgatg gtgagctggg cgctgcagcg aaaaccgtcc atgtaggttg
     3661 ccgacacctt gtacgccggc ccgggcgcca gcccgcgcgc accttgcacg cgtacccggt
     3721 gttcgcccgc ctgctcgagc gtcacctggc tgaagtcgca gctcacgtcc ggcagcaggt
     3781 agcgccgcgg atcgccgatc tcgtagagca tctgctcgcc gatcacagcg gtgttgacca
     3841 gcccgccggt gcctggcggt ttggtcacga tgaagctgcc gtcggcattg cattcgagga
     3901 ccgggtagcc gatgtgcgcc cagtcgggca cggcctccca gtcggtgtgc aggccgccgg
     3961 tggcctggca gccgcattcg atgatgtggc cggccaggct gccctgggcc agacgatcga
     4021 agtcatccgc cggccagccg aattcgtgca tcaacgcacc cagcgtcacg gcgctgtcca
     4081 cgcaacggcc ggtcaccacg atctggccgc cgtcgtcgag cgcctgcttg atcggaagag
     4141 cccccaggta ggcattggcc gtcagcagcc tgtcgggcag gccggctccg ctttgcagct
     4201 cggtcacgcc ttgcgcgcgc agttcgggca cgagcggcat cacgtcgtcg ccctggacca
     4261 cggcgatcct cacttgcacg ccctgttcgg cggccagcgc cgccagcgcg ccggcgcagg
     4321 cccgcgggtt cacgccgccg gcattgctga tgacgcggat gcccctggcc gccacctcct
     4381 tgagcacggc cttcatcgtc accgtcacga agtcggtggc gtagcccagg gccgggtcct
     4441 tggcgcgcgc ggccgccagg atcgacatcg tgagttcggc caggtaatcg aacaccaggt
     4501 agtcgatttc gcccagccgc accagctgcg gcgcgcccac gccggagtcg ccccagaaac
     4561 ccgaggcccc gccgatgcgg accgccttgt cgttcatcgc agctcccggt gcatggctat
     4621 cttcctttcc actgcggctt gcgcttttgc tggaaggctt gctggccttc cttggcgtcc
     4681 tcggtgagcg tgaacagggc gatctggctc tcggtgaacg ccaccgattc ctcgaacggc
     4741 atcgcctcga tcttcttcat cgtgtacaag ccgcgccgga tggccgccgg cgacttgtcc
     4801 agcaagcgct gcagcagcca gtccagcttc gcgtccaggt cgtcggctac gtagttgacc
     4861 aggccgtact cgagggcttg ggccgcggtc agcggctcgc cggtcaggca cagc
//
LOCUS       DASWIY010000231         5133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_877181, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000231 DASWIY010000000
VERSION     DASWIY010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877181"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1233)
                     /locus_tag="VGA59_08480"
     CDS             complement(37..1233)
                     /locus_tag="VGA59_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9719753.1"
                     /translation="MSVLAITRRMKAWLLACITVLALCITVDDSAVAADAPPLRIGVS
                     LSLSGTYSALGEMQANAYRLWELHVNGRGGILGRKVEVVIRDDHSSPKTAASLYEDFV
                     RKDKMDFVFGPYSSPITAAIAPIVDRHGYPTLAAGAASDDIWRQGYTNVFGMWSPAGR
                     YAVGFLAILSESKLRQVAIVTVDDIFALSVAEGARKWAPQYGLQVTAFVVEPKAHPDM
                     ERAANSARKSGADVLLLAGHFNEALQMRQALKQIGWRPAAFYASVGPTLAEYGERLGA
                     DADGSFSTSVWESHEQLRLPDSPQFLRAFVAAYNVTPSYHAATAYAAGQILERAIIKA
                     GTVDRAAVRQALFSLDTSSIIGRHAVDRTGVQTKRFPLIVQWQSGKREIVWPPEVKTA
                     APLLSH"
     gene            complement(1356..2381)
                     /locus_tag="VGA59_08485"
     CDS             complement(1356..2381)
                     /locus_tag="VGA59_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HEX9719754.1"
                     /translation="MARLDINALTLWITAAAAQHPHDLADHVAERLGVTRRTANKALQ
                     RLVELNWLTREGVPRRPVFRAGLLRQVVRSYPLQGLEEDIPWSRDFAPCFDLLPQVHR
                     MVQHAFCELLNNARDHSEGTAVTVSLRQTPSHVQLLVSDDGRGVFDKINEAFALDDPT
                     LAMLELSKGKLTSQPQRHTGRGLFFTSRLADVFDLHANDVAFQHREWDGNGWMPGRSL
                     KRRGTSVYAAIALDTTRTLESVLNNHSLDGAGYGFERTVVPLRLITSTLAGLESRAQA
                     RRVASRLNQFRRAEVDFNGVATIGHGFADELFRVLANDGPNAELVPINMSPSVAAMVN
                     SVRSGTA"
     gene            complement(2479..3684)
                     /locus_tag="VGA59_08490"
     CDS             complement(2479..3684)
                     /locus_tag="VGA59_08490"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX9719755.1"
                     /translation="MDALVYPREKTLGTLTLVIGMLIWVLLIVGTIGIALIYLLFGFI
                     FYVFAHSALIAYIKGNGVELTAQQFPDLRARFDACCDKLGMTDKPQAYVLQGGGLLNA
                     FATRFLGRHYVVLLSDVVDAMEDNPDGVNFYIGHELGHIRMKHLTGRLFRWPALWLPL
                     VGAAYARAQESTCDRHGLACSTSPENAARALGALAAGQRRWRTLDLAAYKKQLRSTSG
                     FWMSFHELTGGYPWLTKRVARVMEPDGRLPGRNGLAYVLALFVPYAGRIGGAAGPLIL
                     VAIIGILAAVALPAYQEYTVRAKVAVAYAGAAPAQTALADYYAVKKSVPQSLEEAGVA
                     EQLSDGSRLSLNPKTMVLTLSTAHGELHMVPSDEGDGKVRWQCMVGDGLRANLAPKAC
                     RNAPPQGQR"
     gene            4038..5105
                     /gene="fba"
                     /locus_tag="VGA59_08495"
     CDS             4038..5105
                     /gene="fba"
                     /locus_tag="VGA59_08495"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589515.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX9719756.1"
                     /translation="MPLVSMRQLLDHAAENGYGIPAFNVNNLEQVQAVMSAADEVGAP
                     VILQASAGARKYAGEAFIKHLIQAAIETYPHIPLVMHQDHGQSPDVCQGAINLGFSSV
                     MMDGSLQADGKTIASYDYNVDVTRKVVDMAHKLGVTVEGELGCLGSLETMKGDKEDGH
                     GTEATMTMDQLLTDPEQAADFVKRTQLDALAIAIGTSHGAYKFSRKPTGDILAIGRIK
                     EINQRIPNTHLVMHGSSSVPQDLLAQIREYGGNMKETYGVPVAEIQEAIKYGVRKINI
                     DTDIRLAMTGAIRKFFAMNPEKFDPRDYLKPAREAAKAICKERYVQFGCEGQAGKITG
                     EPLSTIAARYASGELRQQVAA"
BASE COUNT          898 a         1666 c         1735 g          834 t
ORIGIN      
        1 catgcggtga accgtcttcg tgacgttggc atgttgtcaa tggctcaaca gcggcgcagc
       61 cgttttcact tccggcggcc aaacgatctc gcgcttgccg ctttgccact ggacgatcaa
      121 ggggaagcgt ttcgtttgca cgccagttcg atcgacggca tggcggccga tgatgctgct
      181 ggtgtcgagg ctgaacaagg cttggcggac cgctgcgcgg tccacggtgc ccgccttgat
      241 gatcgcccgt tccaggatct ggccggcggc ataagccgtt gccgcgtgat aggacggcgt
      301 cacgttgtaa gccgcgacga aggcacgcag gaactgcggt gagtcgggca ggcgcaactg
      361 ctcgtgcgac tcccagacag aggtcgagaa cgatccgtcc gcatcggcac ccagccgctc
      421 tccgtactcg gccagcgtcg ggccgaccga cgcatagaag gccgcgggac gccagccgat
      481 ctgcttgagc gcttggcgca tctgaagggc ttcgttgaag tggcccgcca acagcagcac
      541 atcggcgccg gactttcgag cgctgttggc agcgcgttcc atgtcggggt gcgccttcgg
      601 ctcgaccacg aaggcggtga cctgcaagcc gtactgcggg gcccacttgc gcgcgccctc
      661 ggcgacgctg agcgcgaaga tgtcgtcaac ggtcacgatc gcgacctgcc gcaacttgct
      721 ctccgacagg atggccagga atccgaccgc gtagcggccg gccggagacc acattccgaa
      781 gacgttggtg tagccctgac gccatatgtc gtcggaggcg gcaccggcgg cgagcgtggg
      841 gtatccgtgg cgatccacga tgggcgcgat tgccgccgtg atcggactcg aatacgggcc
      901 gaacacgaag tccatcttgt ccttgcgcac gaagtcttcg tagaggctcg cggcggtctt
      961 ggggctgctg tggtcgtcgc ggatgacgac ttcaaccttc ctgccaagaa tgccgccgcg
     1021 gccgttgacg tgcaactccc acagccggta cgcattggct tgcatctcgc ccaaggcgga
     1081 gtacgtgccg gagaggctga gcgagacgcc gatgcgcagc ggtggcgcgt ctgcggcgac
     1141 ggcactgtca tcgaccgtga tgcatagggc cagcaccgtg atgcaggcca gcagccacgc
     1201 cttcatccgc cgggtgatcg caagcactga catgtgaact acaacttcgc ctgaatggcg
     1261 ttggaaagtg tagtccgtcg cgagcgcgtg cagccgctgg gcggctcagt gaatgccctc
     1321 ggaagaggcg aatggatgtg ccgtgtcgcg cggcatcaag ccgtgccgct gcgcacgctg
     1381 ttcaccattg ccgccaccga cggcgacatg ttgatcggca ccagttcggc gttgggcccg
     1441 tcgttcgcca gcacgcggaa cagttcgtcg gcgaaaccat ggccgatggt cgcgacgcca
     1501 ttgaaatcga cttcggcgcg gcggaactga ttcagtcgcg aggccacgcg gcgcgcctga
     1561 gcacgcgact ccaggcccgc cagcgtggag gtgatcagcc gcagcggcac gacggtgcgc
     1621 tcgaagccgt agccggcgcc gtccaggctg tggttgttga ggacggattc cagtgtgcgc
     1681 gtcgtgtcca gtgcgatggc tgcgtacacc gaggtgccgc ggcgcttgag cgagcgcccc
     1741 ggcatccagc cgttgccgtc ccactcgcga tgctggaacg cgacgtcgtt ggcatgcagg
     1801 tcgaacacgt cggccagcct ggaagtgaaa aacaagccgc ggccggtgtg gcgttgcggc
     1861 tggctggtga gcttgccttt gctcaactcc agcatcgcca atgtcgggtc gtccagtgca
     1921 aaggcctcgt tgatcttgtc gaacacgccg cgaccgtcgt ccgagaccag cagttgcacg
     1981 tggctgggcg tctggcgcag cgacacggtg actgccgtgc cctcgctgtg gtcacgggcg
     2041 ttgttgagca gttcgcaaaa tgcgtgctgc accatgcggt gcacctgcgg cagcaggtcg
     2101 aagcagggcg cgaaatcgcg cgaccagggg atgtcttcct ccaggccttg cagcgggtag
     2161 ctgcgcacca cctggcgcaa caggccggca cgaaaaacgg gccggcgcgg cacgccttcg
     2221 cgtgtgagcc agttcaattc aaccaggcgt tgcagtgcct tgttggcggt tcgccgggtc
     2281 acacccaggc gctcggccac atggtcggcg aggtcgtgcg ggtgctgtgc ggccgcagcc
     2341 gtgatccaca gcgtgagagc gttgatgtcg agtcgagcca cggcggtgcc ttgtgggacg
     2401 gggttcccag tgtcggcctc gccgggcgcg acttgagccg gaaaagcagg ggctttccgg
     2461 ctcagttgcg gcgaacgttc agcgctgccc ttgaggcggt gcgttgcggc acgccttggg
     2521 ggccaagttg gcccgcaagc cgtcgccgac catgcactgc cagcgcacct tgccgtcgcc
     2581 ctcgtcgctg ggcaccatgt gcagttcgcc gtgcgccgtg ctgagcgtga gcaccatggt
     2641 cttcgggttg agggacaggc gcgagccgtc gctcagttgc tcggccacgc ctgcctcctc
     2701 cagcgactgc ggaaccgact tcttgacggc gtagtagtcg gccagcgccg tctgggcggg
     2761 cgcggcgccg gcgtaggcca cggccacctt cgcgcgcacg gtgtactcct ggtaggccgg
     2821 cagtgccacg gccgccagga tgccgatgat ggccacgagg atcagcgggc ccgccgcgcc
     2881 gccgatgcgt ccggcatagg gaacgaacag cgccagcaca taggccagcc cattgcggcc
     2941 gggcaaccgc ccatcgggtt ccatcacgcg ggccacgcgc ttggtcagcc acgggtagcc
     3001 gccggtgagc tcgtggaacg acatccagaa gccgctcgtg ctgcgcagct gcttcttgta
     3061 ggcggcgagg tccagcgtgc gccagcggcg ctgtccggcg gccagtgcgc ccagtgcgcg
     3121 cgctgcgttt tcgggcgagg tgctgcaggc caggccgtgc cggtcgcagg tggattcctg
     3181 cgcacgtgcg taggccgcgc cgaccagcgg cagccacagc gcgggccagc ggaacaagcg
     3241 gccggtcagg tgcttcatgc ggatgtggcc gagttcatgg ccgatgtaaa agttgacccc
     3301 gtcggggttg tcttccatcg catcgaccac gtccgacagc agcaccacgt agtgccggcc
     3361 cagaaagcgg gtggcaaagg cgttgagcag gccgccgcct tgcagcacgt aggcctgcgg
     3421 cttgtcggtc atgccgagct tgtcgcagca ggcgtcgaag cgtgcacgca ggtcggggaa
     3481 ttgctgcgcc gtgagctcca cgccgttgcc cttgatgtag gcaatcagcg ccgagtgcgc
     3541 gaacacgtag aagatgaaac cgaacaacag gtagatgagt gcgatgccga tcgtgccgac
     3601 gatcagcagc acccagatca gcatgccgat caccagggtg agggtgccga gcgttttctc
     3661 gcggggataa acaagtgcgt ccatggagtc ctccggtcgg gttcagcgga tggggggctg
     3721 tactccggcc ggccgcatgc gggcatgcga accaaccgcc ccgccgttgc gatgcgcggc
     3781 ggaggccctc cctcttctct tgccgtggca aggggccact tgcacgtggc gcgcggatga
     3841 tacgcacgcc cgcggcggcg cggcggcatg gctttccatg aggctgaggg cattgctcac
     3901 attccccccc gattcgtgcg tgttcgcgcc ccgatcggcc gcagggcggc ccgctaaaat
     3961 cgcgcgttac atcgcgggta catcaccgcg aagccctgcg cgggccaccc tgtttcatca
     4021 actccccggg acacaccatg ccactcgttt ccatgcgcca gctgctggac cacgccgccg
     4081 aaaacggcta cggcataccg gccttcaatg tcaacaacct tgaacaggtg caggccgtga
     4141 tgtcggcggc cgacgaggtg ggggcgccgg tgatcctgca ggccagcgcc ggggcgcgca
     4201 agtacgcggg cgaggccttc atcaagcacc tgatccaggc cgctatcgag acctacccgc
     4261 acattccgct ggtgatgcac caggaccatg gccagagccc cgacgtgtgc cagggcgcca
     4321 tcaacctcgg cttcagctcg gtgatgatgg acggctcgct gcaggccgac ggcaagacca
     4381 tcgccagcta cgactacaac gtcgacgtga cgcgcaaggt ggtggacatg gcgcacaagc
     4441 tgggcgtcac cgtcgaaggc gagctgggct gcctcgggtc gctggaaacc atgaagggcg
     4501 acaaggaaga cggccacggc accgaggcca ccatgaccat ggaccagctg ctcaccgacc
     4561 ccgagcaggc ggccgatttc gtcaagcgca cgcagctcga tgcattggcc attgccatcg
     4621 gcaccagcca cggcgcctac aagttcagcc gcaagcccac gggcgacatc ctggccatcg
     4681 gccgcatcaa agagatcaac cagcgcattc ccaacaccca cctggtgatg catggctcca
     4741 gcagcgtgcc gcaagatttg ctggcgcaga tccgcgagta cggcggcaac atgaaagaga
     4801 cctacggcgt gccggtcgcc gagatccagg aagccatcaa gtacggcgtg cgcaagatca
     4861 acatcgacac cgacattcgc cttgcaatga ccggcgcgat ccgcaagttc ttcgcgatga
     4921 acccggagaa gttcgatccg cgcgactacc tcaagcccgc gcgcgaagcc gccaaggcca
     4981 tctgcaagga gcgctacgtg cagttcggct gcgagggcca ggcgggcaag atcacgggcg
     5041 agccgctgag cacgatcgca gcgcgttacg ccagcggcga gctgcgccaa caagtcgccg
     5101 cctgagcgcc ggtgccagcc cccacgcagc aaa
//
LOCUS       DASWIY010000232        25351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_135199, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000232 DASWIY010000000
VERSION     DASWIY010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 25351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25351
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135199"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="VGA59_08500"
     CDS             <1..1478
                     /locus_tag="VGA59_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9719757.1"
                     /translation="FQSLALTPNLAAKAGLEVFTPIDGSRLACIALATPAEANAAIAD
                     AHRCFLGWRDVPAPLRGQLVRAFGETVRRHKPELGRLISLETGKILQEGLGEVQEVID
                     ICEFAVGLSRQLYGLTIASERPDHKLLETWHPLGVTGVISAFNFPMAVFAWNAALALV
                     CGNTLAWKPSEKTPLSALALNGLLLRAAMDQGVETAGLSELLIGDRGIGEDLVAHPQV
                     KLVSATGSTAMGKSVGIACATLFKRSLLELGGNNAAIVCPSANMDLVVRATAFAAAGT
                     AGQRCTSLRRLIVHRSIYPALVERLVGVYARLPVGNPLDEGTLVGPLIDGRAWDAMQA
                     ALSEVRSLGGKVHFGTRETGPGTGHYVRPAIVELVEQGGPMLRETFAPILYIAPYDRF
                     EDAIAMNNAAAHGLSSALFTQDLREAEAFTSARGSDCGIANVNIGTSGAEIGGAFGGE
                     KETGGGRESGSDAWKAYMRRATNTINYGTSLPLAQGIRFEI"
     gene            1712..1924
                     /gene="rpsU"
                     /locus_tag="VGA59_08505"
     CDS             1712..1924
                     /gene="rpsU"
                     /locus_tag="VGA59_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX9719758.1"
                     /translation="MTMIRVKENEPFDVALRRFKRTIEKLGLLTELRAREFYEKPTAE
                     RKRKKAAAVKRHYKRVRSMQLPKKLY"
     gene            2035..2481
                     /locus_tag="VGA59_08510"
     CDS             2035..2481
                     /locus_tag="VGA59_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX9719759.1"
                     /translation="MSLKERITEDMKAAMRARDAERLGTIRLLTAAMKQKEVDERIEL
                     DDAAVIGIVDKLIKQRKDSVEAFEKAGRKDLADKEAAEIVVLQAYLPARLSAEEIAVE
                     VNAIVGELGAKGPGDMGKVMGAAKARLAGKAEMGQVSAAVKAALAG"
     gene            complement(2614..3990)
                     /gene="pmbA"
                     /locus_tag="VGA59_08515"
     CDS             complement(2614..3990)
                     /gene="pmbA"
                     /locus_tag="VGA59_08515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEX9719760.1"
                     /translation="MNNPDPRAATGFSYSRASFEELVDAALAHAKKLGATDAGAEASE
                     GCGLSVSVRKGELENVERNRDKSLGVTVYVGRRRGNASTSDFSRAAIEQTVQAAYDIA
                     RFTAEDPVAGLPDAEDIAGPGEQPDLDLFHPWDIDGERAAQLALVCEGAAFATDKRIT
                     NSEGAGVSAQQSHFFSAHSRGFRGGYSSSRHSISVAPIAGKGDDMQRDAWYSSMRDAS
                     ELAAPEAVGRYAAERALARLRSRKIATRECPVLFESPLAAGLLGAFVQAVSGGSLYRK
                     SSFLIDSMGKKVFAGHLDIVEDPLVPRGKGSAAFDEEGVRTKARRVVDAGTVAGYFLS
                     SYSARKLGMKTTGNAGGSHNLALTSRLTRPEDDLPEMLRKLGTGLFVIELMGQGVNYV
                     TGDYSRGASGFWVENGELAFPVQEITIAGNMKSMLAGIQAVGADVYNYGAKTVGSVLI
                     DRMTVAGS"
     gene            4016..4651
                     /gene="yjgA"
                     /locus_tag="VGA59_08520"
     CDS             4016..4651
                     /gene="yjgA"
                     /locus_tag="VGA59_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEX9719761.1"
                     /translation="MTRKPKKGYFVRGQFVAEGSELDLELKAELKGTQEASKTDLKRE
                     STELQKLGEALLTLRADLMDGLLLPEKLTDAVAEARRITNFEGKRRQMQYIGKLMRGL
                     DEESLQSVRDALDEQRKGSAGEALALHQAEKWRDDLIGRQETLQQWLSAYPQTDSQQL
                     RALIRQARKDVQPDPDAVSRGLAPRQGRAYREIFLLVKQQLSGHDDAPDSA"
     gene            4648..5262
                     /gene="mog"
                     /locus_tag="VGA59_08525"
     CDS             4648..5262
                     /gene="mog"
                     /locus_tag="VGA59_08525"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEX9719762.1"
                     /translation="MNAPMDTQFDPVRIGIVSISDRASSGVYEDKGLPALKDWLTRAL
                     KNPITFEPRLIPDEKERIGAALIELADAGCSLVLTTGGTGPAPRDVTPEATLAVADRQ
                     MPGFGEQMRQISLRFVPTAILSRQCAVIRGKSLIINLPGQPKSIQETLEGLKGSSGEQ
                     LVPGIFAAVPYCVDLIGGPYLETHDEVCKAFRPKSAIRAPRSST"
     gene            5295..5846
                     /locus_tag="VGA59_08530"
     CDS             5295..5846
                     /locus_tag="VGA59_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9719763.1"
                     /translation="MDPIVTPRRNWSRADIANLLVSSSALADLKLEHALQVVRLLMPQ
                     HKAAGTVLIEEGVTSTGFMALVLEGEAVVANAIPGSSESVLLSHLGPGALFGELGILD
                     GKPRSATVTAVTDMDIVVLDRAGLARLIETVPPVACALLMAIVLRVSERLRATTRMVK
                     ELSVANQLLRKELAAFKRPSSRA"
     gene            complement(6039..6812)
                     /gene="cysE"
                     /locus_tag="VGA59_08535"
     CDS             complement(6039..6812)
                     /gene="cysE"
                     /locus_tag="VGA59_08535"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX9719764.1"
                     /translation="MFTRLRSDIQCILDRDPAARSKWEVLTCYPGLHALMAHRVSHWF
                     WTHGMRWLGRFISQFSRWLTGIEIHPGAKIGDKVFIDHGMGIVIGETAEVGEGCTIYQ
                     GVTLGGTSLTKGAKRHPTLGRNVVVGAGAKILGGFTVGDNASVGSNAVVTKPVPAGAT
                     AVGIPARIIQADDSKRREQVANQLGFSAYGITENDDPLSQAMKGLIDNAASQEHQIAL
                     LWHAIEKLAPARKGEECVPEDAALKEHFEADKLTQLVGK"
     gene            complement(6842..7567)
                     /locus_tag="VGA59_08540"
     CDS             complement(6842..7567)
                     /locus_tag="VGA59_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9719765.1"
                     /translation="MDTRFILINTSHAGNVGAAARAMKVMGFEDLVLVAPRWPDVLVR
                     DETIQRATGATDVLERARVVATLDEALEGLTYLCATAMTPRDFGPPTFTPREHLDGLA
                     KAAHSVGFLFGSERFGMNNEDVYRCHAALTIPTDPRYGSLNLAAAIQVIAYEWRTALG
                     GFGVRPAAKGGQLADAQALAGMLAHWERALVDIGFLDPAAPRKLMPRLNQLFNRAQLV
                     PEEIHILRGVAKAMSEAASRVKR"
     gene            7847..8755
                     /locus_tag="VGA59_08545"
     CDS             7847..8755
                     /locus_tag="VGA59_08545"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX9719766.1"
                     /translation="MSSPNLHPMLNVAVKAARAAGAIINRAALDVESVRISQKQVNDF
                     VTEVDHASEQTIIETLLTAYPHHAIWAEESGKVHGTQGSDFVWIIDPLDGTTNFIHGF
                     PVYCVSIGLSVKGKVEQAVIYDPSRNDLFTATKGRGAYMNERRMRVSKRTELKQCLIS
                     TGFPFRAGDNMKNYLRMMAEVMPRTAGLRRPGAAALDLAYVAAGFTDGFFETGLSPWD
                     VAAGSLLVAEAGGLIGNFSGDSDFMEQKECIAGNPKIYGQLVAILGKYSKFASAGEKA
                     ALRHATLALVPNEHKPAERVDDAAES"
     gene            8772..9866
                     /locus_tag="VGA59_08550"
     CDS             8772..9866
                     /locus_tag="VGA59_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX9719767.1"
                     /translation="MELTELVQRNPWTATAVVALVAVFAALVAYRLGRAVLVRITGGV
                     PMLQAVLEATERAAGAALPLAALQLVWTAAPDDLPYIETARHINGLLLIGALTWLIMG
                     VIAGVANGVIARHPATVEDNLNARRVQTQAKVLSRSAMVLVLLAGTAMALMTFPGARQ
                     LGASLLASAGVLGIIGGLAARPVFSNLIAGLQLALAQPIRIDDVLIVKGEWGRVEEIT
                     GTYVVLKIWDERRLIVPLQWFIENPFENWTRTSSQILGTVFLNLDFAMPMDALRAELE
                     RVVHAAPEWDKRVCGLQVTDATDRTMQVRLLVSAASAGAAFDLRCKVREALLAFVARE
                     HPQCLPRVRLLDGDPPQEAAMTRGHARATG"
     gene            9946..12510
                     /gene="mutS"
                     /locus_tag="VGA59_08555"
     CDS             9946..12510
                     /gene="mutS"
                     /locus_tag="VGA59_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX9719768.1"
                     /translation="MMAQYLAIKAEHPQTLVFYRMGDFYELFYSDAEKAARLLDITLT
                     KRGNSAGEPVVMAGVPFHSVEGYLARLIRQGESVAICEQVGDVATAKGPVERKVVRVV
                     TPGTLTDTELLNDKAEALLLAVHQGSRHRLGLAWLSVTQGALQLAECAADELESWVVR
                     IAPSELLFGADVTTAFEQRLKALRFATPLSLTQRPEWQFEAGLGLRKLLEQLNSASLA
                     AWGAEELVHAHAAAAALLSYAEHTQGRALSHVQSLQVQRDDELIDLPVTTRRNLELVQ
                     TLRGEDAPTLFSLLDTCMTGMGSRMLKGWMLQSRRDRGEARQRHEAVAVLRERLWQVL
                     RAELKGCSDVERITARVALRQVRPRELVGLAQSLQKSAQLARQLAGQDALLAGIAADM
                     DPPAGCAGMLARTLLEEPAALARDGGVIASGLDTELDELRAIQDNCDGFLLELETRER
                     SRTGIANLRVQFNKVHGFYIEISQGQLDKVPGDYRRRQTLKNAERFITPELKAFEDKA
                     LSAQDRALAREKWLYEQLLDQLQAFVPALTRLARALASLDALCALAERSLTLGWCRPH
                     FMKEPGIEILQGRHPVVQARLAETSGGAFIANDTRLGPKQRMQIITGPNMGGKSTYMR
                     QVALVCLLASMGSYVPAAECRLGPMDAIHTRIGAADDLANAQSTFMLEMTEAAQILHA
                     ATPQSLVLMDEIGRGTSTFDGLALASGIAAHLHDKTQAFTLFATHYFELTGFPARHHA
                     ALNVHVSAAESGSDIVFLHEIQPGPASRSYGIQVARLAGMPVAVVNHARHALAALETQ
                     QTLSREQVDLFAAPPTIEASAPSAVEAAVAALNPDALSPREALDALYQLKKLGGKP"
     gene            12507..13334
                     /locus_tag="VGA59_08560"
     CDS             12507..13334
                     /locus_tag="VGA59_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HEX9719769.1"
                     /translation="MTYCVGIKLNAGLVFLSDSRTNAGVDNISTFRKMIIYERPGDRF
                     MVLLSAGNLSIAQSVREILQVEQLKEHPDSEVINIWNAKSMFDAARVLGNAVRHVYDR
                     DAEALKNAGVEFNVQLIFGGQIRGESMRLFQVYSAGNFIEATPETPYFQVGEYKYGKP
                     VLDRVITPDTPLDEAAKCALVSMDSTMKSNLSVGPPLDLVVYEVNRFETDKIVCVDMQ
                     NPYYRMLHSSWGQKLREVFDSLEDPVWDDAHTDVPLKVVDARSKPLKKITRPDERLI"
     gene            13478..14482
                     /gene="dctP"
                     /locus_tag="VGA59_08565"
     CDS             13478..14482
                     /gene="dctP"
                     /locus_tag="VGA59_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9719770.1"
                     /translation="MNRRPLFRTLAGLLSAAIVSAPALADTITLRASHQFPGGKGDVR
                     DEMVQIIAREAAAANVGLEIKVFPGSSLVKAQEQWKAMQTGQIDMTSFPLDYASGFHP
                     QFGATLMPGLVKNHEHAARINASPFMQDIKAIIEQGGVKVLADAWLAGAFASKDKCIR
                     NPEDAAGLKVRSAGATFSQMWAGAGASIVSIPSNEVYNALQQGVAQATDTSSSSFVSF
                     RLYEQLKCLTAPGDNALWFMYEPVLISKRSWDKLNDAQKKAVMAGSKKAEAYFSAESK
                     KLDDKMVDTFKKNNVQVVTMTDAEAEAWRAVARKSSYKIFAEKVPGGKELIDKALSVK
                     "
     gene            14472..15011
                     /locus_tag="VGA59_08570"
     CDS             14472..15011
                     /locus_tag="VGA59_08570"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9719771.1"
                     /translation="MSSDAAPPPAWVAAVHSVSRLFGVVAAGMILVSIGVVCHMVFVR
                     AVLGQSSIWQTEFVTFSLVAATFIGAPYILLTRGHVSVDVVPLIVRDRTRRVLHFIGY
                     ASALAFCALFLYAAVPWWYESWQLGQTTPSIWRARLWIPYLSVPVGLALLCLELLAEI
                     WMVLSRRALPFGLGRDEGP"
     gene            15040..16347
                     /locus_tag="VGA59_08575"
     CDS             15040..16347
                     /locus_tag="VGA59_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9719772.1"
                     /translation="MSPIAIGLLVFVATCGILATGLPIAFGLGVVALTFMVIFDGWNS
                     IHFLPETVFSGLSDFTLVSLPMFVIMGAAVASSRAGSDLYEALARWLHRVPGSLVISN
                     IGACALFSALTGSSPATCAAIGKMGIPEMRRRGYDADLATGAIAAGGTLGILIPPSIT
                     MILYGIASETSIGRLFIAGIIPGLLLTGLFMAWALFLSWKRGTRLVDSGRIYTWKEKF
                     ELLPRLLPFIAVIVGVVYALYGGIATPSEAAGVGAMLTLVMVVVIYRVWRPAHLWAIL
                     RDGLRESGMLLLIIGTSILFGYMLSSLQVTQSVAQSIGELHVNRWIVLAAINLLLLVA
                     GCFLPPAAIILMTTPILMPVISAAGFDPIWFGVILTINMELGLITPPVGLNLFVINGI
                     TPDVKLPTILKGAFPFMLCMVAAIVILCVFPELATWLPSRLMG"
     gene            16403..17212
                     /locus_tag="VGA59_08580"
     CDS             16403..17212
                     /locus_tag="VGA59_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9719773.1"
                     /translation="MSLIIFSHGNSFPAATYGALFKNLRPRGFVVRAVEKFGHDPDYP
                     VTDNWRHLVQQLHDFTAREVDRHGEPAFLVGHSLGGFLSLMCAARHPQLARGVLLLDA
                     PLLGGWKATALGAIKRSQLVGSISPGRISRQRKHRWPSREAALEHFRHKKAFANWDPQ
                     VLHDYVTHCMHDEGGERVLSFDRDVETAIYNTLPHNLDRLLGRHPPKCPVAFIGGRQS
                     EEMKRVGMQMTQQVTKGRIMMIDGSHLFPMEKPVATAAAIEAALRGFDFPV"
     gene            17325..18101
                     /locus_tag="VGA59_08585"
     CDS             17325..18101
                     /locus_tag="VGA59_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9719774.1"
                     /translation="MDSTQTFSHPVHAPSISLLVMEPLRALFDHFAGRIGCEALPLGD
                     GHPVVVYPGLGGGALTTSHLRRFLSDSGFTVHDWEGGVNTGPGGIFDDWLGGLEQRVR
                     GLHREHGRKASLVGWSLGGVYAREIAKRCPESVRQVITLGTPFAALGGGNHATAVYKL
                     LNRDHTQLTPELQARLRECPPVPTTSIFSKTDGIVSWRGCIEKKSVSSESVEVAASHL
                     GMGTHPQVLRIVANRLAQPEGRWRALRRSERLGRPRLARG"
     gene            complement(18102..18926)
                     /locus_tag="VGA59_08590"
     CDS             complement(18102..18926)
                     /locus_tag="VGA59_08590"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX9719775.1"
                     /translation="MDFVLLAKAAVMGVVEGLTEFLPVSSTGHLILAGALLGFHDDKA
                     KVFDIAIQTGAILAVIIVYWQKIRQTLVALPTQRQAQQFSLNVLIAFFPAVVLGLLFG
                     KAIQANLFTPAVVASAFILGGLVILWAEKRQQSAVRIHDVDQMSWKDALKVGLVQCLA
                     MIPGTSRSGATIIGAMLLGLSRRAATDFSFYLAIPTLIGAGAYSLYKERALMTVDDLP
                     IFTVGLLFSFISAWMCVRWLLRFISTHSFTPFAWYRIAFGIVVLATAWSGLVTWAP"
     gene            19259..19663
                     /locus_tag="VGA59_08595"
     CDS             19259..19663
                     /locus_tag="VGA59_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX9719776.1"
                     /translation="MTQLTPLIAIHMTAALLAVVTGPVALWARKGATQRPKLHRSFGY
                     AWVTLMLIAAGSAFSIRGGELPNIGGFSPIHLFIPVVLFGLVGAFWFLAKGNIRAHRR
                     TMLGLYLGACLGAGAFALLPSRLLGQFLLGLA"
     gene            19697..20263
                     /locus_tag="VGA59_08600"
     CDS             19697..20263
                     /locus_tag="VGA59_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6622 family protein"
                     /protein_id="HEX9719777.1"
                     /translation="MLIQMLIERPQMLGRVLNGTPPWVWGLLAGLVLLGLSQGRARTA
                     GLARIAILPVAMIVLAVWGLMSAFRGSPQLGSVLLAWFAAAALSLAATASMAPPAGAR
                     YDAVSRMFSLPGSWVPMLLILGIFLTKYIVGVELAMQPALAHDGQYSLVVSAIYGLFS
                     GIFTGRAVRLWRLASRPASAATAQPINA"
     gene            20291..20587
                     /locus_tag="VGA59_08605"
     CDS             20291..20587
                     /locus_tag="VGA59_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEX9719778.1"
                     /translation="MNALTQLDERGLEKLARKRAGAKLGWYIHALVYTCVNLLLAMLS
                     AASGGDWAVFPALGWGIGLAAHGLVTFVVTGGAGLHERLVQRERRRLTLQRDPW"
     gene            20805..21098
                     /locus_tag="VGA59_08610"
     CDS             20805..21098
                     /locus_tag="VGA59_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEX9719779.1"
                     /translation="MRTLSSEDLERIAHKRAGAKLGWYVHAALYIVVNLFVFAISSYG
                     FGERPWSVFPLLGWGLGLGLHGISVFMLGAGGEFRERLVKQERDRLRRHQDGS"
     gene            21064..22152
                     /locus_tag="VGA59_08615"
     CDS             21064..22152
                     /locus_tag="VGA59_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9719780.1"
                     /translation="MTDCAGTKTAREAAGMKIDWVVKLQHLLQVLAFCLAVATIQYGF
                     QPDRPYAPNVVHSLLIGSTMWAIIDLGRHLMPSSAETGWPQGIAGLALVVGGITAGYL
                     LGTTIADRLCVYYGWYPAGTRDNSAANWRTSILITVLAGSVGTFYFYSVNKSAYLKRK
                     MTEASQHATEARLKLLETQLEPHMLFNTLANLRALIGSDPRQAQEMLDHMIAYLRATL
                     SASRTTTHSLQAEFDRLRDYLELMTVRMGPRLDYSLELPPELAQHPVPALLLQPLVEN
                     SIQHGLEPKVEGGRVTVSARREDGRLVLEVADSGVGPSGVTADGKGFGMNQVRERLAT
                     LHGAAARLDFAAAPGGGARTILSLPLQS"
     gene            22149..22973
                     /locus_tag="VGA59_08620"
     CDS             22149..22973
                     /locus_tag="VGA59_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX9719781.1"
                     /translation="MNAAGATALIAEDEPLLARELQAELARAWPQLRIVATVGDGASA
                     VAQALALKPDVLFFDIRMPGQSGLEAAVELADEWPQELPFPALVFVTAYDQYAVQAFE
                     TEAVDYVLKPVQAARLQKTVAKVQDVLARRARGTVDFDATLGQLRSLLGSVAPTAPAA
                     AGPPNEGPLKMIQVSVGNSIRMVPVEEVLYFEAADKYVRVLTEGHEYLIRTPLKELTP
                     QLDAGRFWQVHRGTVVRADAIDSVTRDEAGKLHLQLRGRSEKLAVSRLYTHLFKAM"
     gene            complement(23329..23718)
                     /locus_tag="VGA59_08625"
     CDS             complement(23329..23718)
                     /locus_tag="VGA59_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719782.1"
                     /translation="MNSSRSVYVKVAAAALALAAAGTASVAQARSDVYYSIGAHVAPG
                     VSIGVSNAPVYYPPVYLQPAPVYYPPAQYYVRPAPVFVPAPAYYARPAPIYYVRPAPV
                     YGAGYYYDYGPRGHWKANKHRKHRGRH"
     gene            23888..>25351
                     /gene="glnS"
                     /locus_tag="VGA59_08630"
     CDS             23888..>25351
                     /gene="glnS"
                     /locus_tag="VGA59_08630"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HEX9719783.1"
                     /translation="MTSAADKEPLKPSNFLRQVIERDLEQGTYAQRRWGGSPGDAAHH
                     IQGQPDPARIRTRFPPEPNGYLHVGHAKSICLNFGLARDYGGVCHMRFDDTNPEKEDL
                     EYVNSILEAVTWLGFSWEANGTSHYYHASDYFDFMYRAAEYLIEAGLAYVDEQTPEEM
                     RAHRGDFNKPGTDSPFRSRSIDENLQRFRDMQGGKLADGAAVLRAKIDMASRNINLRD
                     PAIYRIRRATHHNTGDKWCIYPMYTYAHPIEDALENITHSICTLEFEDQRPFYDWLLD
                     RLGEGGLTNQPHPRQYEFARLNLTYVITSKRKLKQLVDEKHVSGWDDPRMPTIVGLRR
                     RGYTPESIQLFAERIGVTKSDSWIDYATLEQALRDDLDPKAPRAMAVLDPVKLVITNW
                     GQVMGSDDKLDDCSAPLHPNNPEMVGRRDFKFGRELWIERTDYEEVPPKGYNRLYPGN
                     KVRLKYGHVIECKGAVKDKDDRVTVVRAELIPDTKSGT"
BASE COUNT         4364 a         8581 c         8242 g         4164 t
ORIGIN      
        1 tctttcaatc cctcgcgctc acgccgaacc tcgccgcaaa ggccgggctc gaagttttca
       61 cacccatcga cggcagccgt ctggcctgca ttgcgttggc cacccccgcc gaggccaatg
      121 ccgccatcgc ggacgcacac cggtgcttcc tgggctggcg tgacgtgcca gcacccctgc
      181 gcggccagct ggtgcgcgcc tttggcgaaa cggtgcggcg gcacaagccc gagctcggcc
      241 ggctgatctc gctggaaacg ggcaagatcc tgcaggaagg cttgggcgag gtccaggagg
      301 taatcgacat ctgcgaattc gccgtcggcc tgtcgcgcca gctctacggc ctgacgatcg
      361 ccagcgaacg ccccgaccac aagctgctgg aaacctggca cccgctcggg gtgaccggcg
      421 tgatttcggc cttcaacttc ccgatggccg tgttcgcgtg gaatgccgcg ctggccctgg
      481 tgtgcggcaa cacgctggcc tggaaaccct cggagaagac cccgctgagc gcactggccc
      541 tgaatgggct gctgctgcgc gccgcgatgg atcaaggcgt ggaaaccgcg ggcctgagcg
      601 agttgctcat cggtgaccgg ggcatcggcg aggacctcgt tgcccatcct caggtgaagc
      661 tggtcagcgc gaccggttcc acggcgatgg gcaagagcgt gggcatcgct tgcgcgacgc
      721 tgttcaagcg atcgctgctc gagctgggtg gcaacaacgc cgccatcgtc tgcccctcgg
      781 cgaacatgga cctggtggtg cgtgctaccg cgtttgccgc cgccggcacg gcgggccagc
      841 gctgcaccag cctgcgccgg ctgatcgtgc atcgctccat ctaccccgcg ctggtggagc
      901 gcctggtggg cgtgtatgcc cgcctgccgg tgggcaatcc gcttgacgag ggaacactgg
      961 tcggcccgct gatcgatggc cgcgcctggg acgcgatgca ggccgcgctg tccgaggtcc
     1021 gctcgctcgg tggcaaggtc cacttcggca cacgcgaaac cggaccgggc acgggacact
     1081 atgtgcggcc ggcgatcgtc gagctggtcg agcagggcgg cccgatgctg cgcgaaacct
     1141 tcgcgcccat cctctatatc gcgccgtatg accggttcga ggacgcgatc gcgatgaaca
     1201 acgccgccgc ccatggcctg tcctcggccc tcttcaccca ggacctgcgc gaggccgagg
     1261 cgttcacgtc ggcgcgcggc tccgactgcg gcatcgccaa cgtcaacatc ggcaccagcg
     1321 gcgcggaaat cgggggcgcc ttcggcggcg agaaggaaac cggcggcggc cgcgagtccg
     1381 gctcggatgc ctggaaggcc tatatgcgcc gcgccaccaa cacgatcaac tacggaacca
     1441 gcctgcctct ggcccagggc atccgtttcg agatctgacc tgcgggatcc cgcccgcccc
     1501 gaaatggggc atttgccccc gcgtggaggg aagattgagc gcgccggcgg gacttcctgc
     1561 ctgccgcccg cccttgtggg gctataatca cgggctttgc tggcaaaccc tcaacggcct
     1621 gatcaccttc agttggggaa cccgcggcca aggcgcatgg gcccgatctt cccgcgcacc
     1681 ctctggctgc acatttggaa ccgattagct aatgacaatg atccgtgtga aagagaatga
     1741 gccgttcgac gtggccctgc gccgcttcaa gcgcaccatc gagaagctgg gcctcctgac
     1801 cgaattgcgc gcgcgcgaat tctatgaaaa gcccaccgcc gagcgcaagc gcaagaaggc
     1861 ggcagcggtc aagcgccact acaagcgcgt gcgcagcatg cagctgccca agaagctcta
     1921 ctagggcatc tgcttggcgc cccccgcgga caggctcaaa ggcctgccgc gcattcaagc
     1981 ccgcctgggg acgctcaagc gggctttgtt gtttccgtca gccggagaaa gccgatgagc
     2041 ctgaaagaac gcatcaccga agacatgaag gccgccatgc gcgccaggga cgccgagcgc
     2101 ctgggcacca tccgcctgct cacggccgcc atgaagcaga aggaagtgga cgaacgcatc
     2161 gagctggacg acgccgccgt gatcggcatc gtggacaagc tgatcaagca gcgcaaggac
     2221 agcgtggaag ccttcgagaa ggccggccgc aaggacctgg ccgacaagga agccgccgag
     2281 atcgtcgtgc tgcaggccta tttgccggcg cggctttccg ccgaggagat tgccgtcgag
     2341 gtcaatgcca tcgtgggcga gctgggcgcc aagggcccgg gcgacatggg caaggtcatg
     2401 ggcgccgcga aggcgcggct ggccggcaaa gccgagatgg ggcaggtctc ggcggcggtg
     2461 aaggctgctt tggctggata gccatccgaa ccttccacga gcttgtcatg ccggacttga
     2521 tccggcatcc aggcggcgcg tgcatggatc ccggccttcg ccgggatggg aggctctcgc
     2581 gcctggatcc cggctgcgcc gggaaggcag gcgttacgac cccgccaccg tcatccggtc
     2641 gatcagcacc gagcccaccg tcttcgcgcc atagttgtac acatcggcgc ccaccgcctg
     2701 gatgccggcg agcatggatt tcatgttgcc cgcgatggtg atttcctgca ccgggaacgc
     2761 gagttcgccg ttctccaccc agaagccgct cgcgccacgc gagtagtcgc cggtgacgta
     2821 attgacgccc tggcccatca gctcgatcac gaacaggccc gtgcccagct tgcgcagcat
     2881 ttccggcagg tcgtcctccg ggcgcgtcag gcgcgaagtc aacgccaggt tgtgcgagcc
     2941 gccagcgttg cccgtggtct tcatgcccag cttgcgggcc gagtaactgc tcaggaagta
     3001 tccggcaacc gttcccgcat ccacgaccct gcgcgccttg gtccgcacgc cctcctcgtc
     3061 gaaggcggca ctgcccttgc cgcgcggcac cagcgggtcc tccacgatgt ccaggtggcc
     3121 ggcgaaaacc ttcttgccca tcgagtcgat caggaagctg ctcttgcggt acagcgagcc
     3181 gccgctgact gcctgcacga aagcacccag cagcccggcc gccagcggag actcgaacag
     3241 caccgggcat tcgcgagtgg cgatcttgcg cgacctcagg cgggccagcg cgcgttccgc
     3301 ggcgtagcgg ccgacggcct cgggcgcggc cagctcgctg gcgtcgcgca tcgagctgta
     3361 ccaggcatcg cgctgcatgt cgtcgccctt gccggcgatc ggcgccacgg agatcgaatg
     3421 gcgcgagctc gagtagccgc cccggaaacc gcgcgaatgg gcgctgaaga aatggctctg
     3481 ctgcgccgac acgcccgcgc cttcgctgtt ggtgatgcgc ttgtcggtgg cgaacgccgc
     3541 gccctcgcag accagggcca gctgggccgc ccgttcgccg tcgatgtccc aggggtgaaa
     3601 cagatccagg tcgggctgct cgccggggcc ggcgatgtcc tcggcatcgg gcaggccggc
     3661 caccgggtct tcggcggtga agcgcgcgat gtcgtaagcc gcctgcacgg tctgctcgat
     3721 ggccgcccgc gaaaaatccg aagtgctcgc gttaccgcgg cgacgcccga cgtacaccgt
     3781 cacgcccagc gacttgtcgc ggttgcgctc cacgttctcg agttcgccct tgcgcaccga
     3841 cacgctcagg ccgcagcctt cggacgcctc ggcgccggcg tcggtcgcac ccagcttctt
     3901 ggcgtgggcc agggcggcgt ccaccagttc ctcgaatgat gctcggctgt agctgaaacc
     3961 ggtggcggcg cgggggtcgg gattgttcat agcggggcta tgatacttgc cccacatgac
     4021 ccgcaaacct aaaaaaggct acttcgtgcg cggccagttc gtcgccgaag gcagcgagct
     4081 ggatctcgag ctgaaggccg agctcaaagg cacccaggaa gccagcaaga ccgacctgaa
     4141 acgcgagagc accgagctgc aaaagctcgg cgaggccttg ctgaccctgc gcgccgacct
     4201 catggacggc ttgctgcttc ccgaaaagct caccgacgcg gtggccgagg ccaggcgcat
     4261 caccaatttc gagggcaagc gccgccagat gcagtacatc ggcaagctca tgcgcggcct
     4321 ggacgaggaa tcgctgcaat ccgtgcgcga tgcactcgac gagcagcgca aaggctcggc
     4381 cggcgaggcg ctggcgctgc accaggccga gaaatggcgc gacgacctga taggcaggca
     4441 agagaccctg cagcaatggc tgagcgccta tccgcagacc gactcgcagc aattgagggc
     4501 gttgatccgc caagcccgca aggacgtcca gcccgacccg gacgcggttt ccagggggct
     4561 ggccccccgg cagggtcgtg cctaccggga aatcttcctg ctggtgaagc agcagctttc
     4621 cggccatgat gacgcgccgg attccgcatg aacgcaccca tggacaccca attcgacccc
     4681 gtccgcatcg gcatcgtctc gatcagcgac cgcgcctcct ccggcgttta cgaagacaag
     4741 ggcctgcctg ccctgaagga ctggctgacg cgggcgctga agaacccgat cacgttcgag
     4801 ccgcgcctga ttcccgacga gaaggagcgc atcggcgccg cgctcatcga actggccgac
     4861 gcgggctgct cgctggtgct gaccaccggc ggcaccggcc cggcgccgcg cgacgtgacg
     4921 cccgaggcga cgctggcggt ggccgacagg caaatgccgg gctttggcga acagatgcgc
     4981 cagatcagcc tgcggttcgt gccgacggcc atcctttcgc gccaatgcgc ggtgatccgc
     5041 ggcaagagcc tgatcatcaa cctgccgggg cagcccaagt cgatccagga aacgctggaa
     5101 ggcctcaagg gcagcagcgg cgagcaactc gtgcccggca tcttcgcggc cgttccttac
     5161 tgcgtggacc ttatcggcgg gccgtacctg gaaacgcacg atgaggtgtg caaggcattc
     5221 cggcccaagt cggccatccg ggcgccgcgt tcatcaacct gagccgatga cggccggcca
     5281 ggagagcgca accaatggac ccgatcgtca cgccccggcg caattggtcc cgcgccgaca
     5341 tcgcgaacct gctggtctca agcagcgcgc tggccgacct gaagctggaa catgcactgc
     5401 aggttgtgcg gctgctcatg cctcagcaca aggccgcagg cacagtgctg atcgaggaag
     5461 gcgtgaccag caccggtttc atggcgctgg tgctcgaggg cgaagcggtc gtggccaatg
     5521 cgataccggg ttccagcgaa tcggttctgc tgagccatct tggccccggt gcgctcttcg
     5581 gcgagctcgg catcctggat ggcaagcccc gctcggccac ggtcacggcc gtgaccgaca
     5641 tggacatcgt cgtgctcgac cgggccgggc tggcgcgcct gatagaaacc gtgccccccg
     5701 tggcctgcgc cctgctgatg gcgatcgtct tgcgcgtgtc cgagcggctg cgcgccacca
     5761 ctcgcatggt caaggaactg tcggtggcga accagttgtt gcgaaaggag ctggcggcat
     5821 tcaagcgccc gtcatcccgg gcttgaccgg gtttctgcgg atttgtcacc tcgggctcga
     5881 cccggtttcc gcggatttgt catcccgggc tcgacccggg atccatgact tcctggattg
     5941 cgggtcaagc cctggtcaag cccgcaatga cgattgacat ggctgtcatc ccgggctcga
     6001 cccgggatcc aggctcgcgg ccggcgctac ccgcgaactc acttcccgac cagctgcgtc
     6061 agcttgtcgg cctcgaaatg ctctttcaag gcggcgtctt cgggcacgca ctcctcgccc
     6121 ttcctggcag gagcgagctt ttcgatggcg tgccacagca gcgcgatctg gtgctcttgc
     6181 gacgcggcat tgtcgatgag ccccttcatc gcttgcgaca gcggatcgtc gttctcggtg
     6241 atgccatagg cggaaaagcc cagctggttg gccacctgct cgcgccgctt gctgtcgtcg
     6301 gcctggatga tgcgggccgg gattcccacg gccgtcgcgc cggcgggcac cggcttggtc
     6361 accaccgcat tggagcccac gctggcgttg tcgcccaccg tgaagccgcc cagtattttc
     6421 gcgccggcac cgacgaccac gttgcggccc agagtgggat ggcgcttggc gcccttggtc
     6481 aatgaggtgc cgcccagcgt cacgccctgg tagatcgtgc aaccctcgcc cacttccgcg
     6541 gtctcgccga tcacgatacc catgccatgg tcgatgaaca ccttgtcgcc gatcttcgca
     6601 cccggatgga tttcgattcc ggtgagccag cgcgagaact gcgagatgaa gcgcccgagc
     6661 cagcgcatcc catgggtcca gaaccagtgg gagacgcggt gcgccatcaa tgcatgcagg
     6721 cctggataac aagtcagcac ctcccacttg ctgcgcgcgg cagggtcgcg gtcgaggatg
     6781 cactggatgt cggaacgcag gcgggtgaac atgggcaagc ctgaggttgg atatgcgcag
     6841 tctatcgttt taccctggag gccgcttcgg acatcgcctt ggcaacacca cgcaggatgt
     6901 ggatttcctc ggggacaagc tgggcccggt tgaacaactg gttcaggcgc ggcatcagct
     6961 tcctgggcgc cgccgggtcg agaaagccga tgtccaccag cgcgcgttcc cagtgcgcca
     7021 gcatgccggc cagggcctgg gcgtcggcga gctggccgcc ttttgccgcc ggccgtaccc
     7081 cgaacccgcc cagagccgtg cgccattcgt aggcgatcac ctggatcgcc gcggccaggt
     7141 tgagggagcc gtagcgcgga tcggtgggaa tggtcaaggc ggcatggcag cggtagacgt
     7201 cctcgttgtt catgccgaaa cgctcggacc cgaacaggaa gccgacgctg tgcgcggcct
     7261 tggccaggcc gtccaggtgc tcgcggggcg tgaacgtggg cgggccgaag tcgcgcggcg
     7321 tcatcgccgt ggcgcacagg taggtgaggc cttccagcgc ctcgtccagc gtcgccacga
     7381 cccgcgcccg ttccagcacg tcggtggcgc cggttgcgcg ctggatcgtc tcatcgcgca
     7441 ccagcacgtc gggccagcgc ggcgcgacca gcacaaggtc ctcgaagccc atgactttca
     7501 tggcccgcgc ggcggcgccc acgttgcccg cgtggctggt attgatcagg atgaagcggg
     7561 tatccatggg cggattgtct cagcccgcca caggccgaca ccgggccggc gattcgccga
     7621 gccactgcgt ccggcgtttc atgggaaaat gcgggcagtc aatggcgtcc gcatcgccgg
     7681 tccgcatcgc cgctcccggg aacagcgctg aagatctgtc ccacaaactt agtcagttgg
     7741 ggacagagca aaattgctga agcaattctt gatctgtccc gcaaggtatc agaatcagtt
     7801 ggggacagag ctaaagatct gtcccacaaa attcttaaat taattcatgt cgtcccccaa
     7861 tctgcatccc atgctcaatg tggccgtcaa ggccgcccgt gccgccggcg ccatcatcaa
     7921 ccgcgccgcg ctcgatgtgg aatcggttcg catctcgcaa aaacaggtca acgatttcgt
     7981 caccgaagtg gaccacgcga gcgagcagac catcatcgaa accctgttga ccgcctatcc
     8041 ccaccacgcc atctgggccg aggagtccgg caaggttcat ggcacgcagg gttccgactt
     8101 cgtctggatc atcgacccgc tggacggcac gaccaacttc atccacggct ttccggtgta
     8161 ctgcgtcagc attgggctgt cggtcaaagg caaggtggag caggcggtca tctacgatcc
     8221 cagccgcaac gatctgttca ccgccaccaa gggccgcggc gcctacatga acgagcgccg
     8281 catgcgcgtt tccaagcgca ccgaactgaa gcaatgcctg atctccaccg gctttccgtt
     8341 ccgcgccggc gacaacatga agaactacct gcgcatgatg gccgaggtga tgccgcgcac
     8401 cgcgggcctg cgccgccccg gcgccgccgc actggacctg gcctatgtgg ccgccggctt
     8461 caccgacggg tttttcgaga ccggcctgtc accctgggac gtcgccgcgg gttcgctgct
     8521 ggtggccgag gcgggcgggc tgatcggcaa cttcagcggc gactccgact tcatggagca
     8581 gaaggaatgc atcgccggca atcccaagat ctatggccag ctggtcgcga tcctgggcaa
     8641 atacagcaag ttcgccagcg ccggcgagaa ggccgcgctg cgacatgcaa cccttgcact
     8701 ggtgcccaac gaacacaagc ccgccgagag ggtggacgac gccgccgaat cctgatccgc
     8761 aaggacccgg catggaactg accgaactgg tgcaacgcaa cccctggacc gccaccgccg
     8821 tggtggccct ggtggcggtg tttgccgcgc tcgtggcgta tcggcttggc cgcgcggtgc
     8881 tggtgcgcat caccggcggc gtgccgatgt tacaggccgt gctcgaggcc actgaacgcg
     8941 ccgccggcgc cgccctgccg ctggccgcgc tgcagctggt gtggaccgcc gcccccgacg
     9001 acctgcccta catcgaaacc gcgcgccaca tcaatggcct gctgctgatc ggcgccctga
     9061 cgtggctgat catgggcgtg attgccggcg tcgccaatgg cgtgatcgcg cgccacccgg
     9121 ccacggtgga ggacaacctt aacgcgcggc gcgtgcagac gcaggccaaa gtgctctcgc
     9181 gcagcgcgat ggtgcttgtg ctgctggccg gcacggccat ggcgctcatg acctttcccg
     9241 gcgcgcggca gctcggcgcc agcctgctgg cctcggccgg cgtgctgggc atcatcggcg
     9301 gcctcgcggc gcggccggta ttcagcaacc tgatcgccgg gctgcagctg gcactggccc
     9361 agccgatccg catcgacgac gtgctgatcg tcaagggcga atggggccgc gtcgaggaga
     9421 tcaccggcac ctatgtcgtg ctgaagatct gggacgagcg gcgcctgatc gtgccgctgc
     9481 agtggttcat cgagaaccct ttcgagaact ggacacgcac cagctcgcag atcctgggca
     9541 ccgtcttcct gaacctcgac ttcgcaatgc cgatggatgc gctacgggcc gaactcgagc
     9601 gcgtcgtgca cgccgcgccc gaatgggaca aacgcgtgtg cggtttgcag gtgaccgacg
     9661 ccaccgatcg cacgatgcag gtgcgcctgc tcgtttccgc ggcgtcggcc ggcgccgcat
     9721 tcgacttgcg ctgcaaggtg cgcgaagccc tgctggcctt cgtggcccgc gagcatccgc
     9781 agtgcctgcc ccgtgtacgg ctgctggatg gcgacccgcc gcaagaggcc gccatgaccc
     9841 gtggccacgc gcgcgccacc ggataatgcg ggcatcgaac gcatcgccgc cacatccttg
     9901 aaccgcccac ttgcttcctc cgccggcgcc ccggcgcaca cgcccatgat ggcgcagtac
     9961 ctcgccatca aggcggagca tccgcagacg ctggtgttct accgcatggg cgacttctac
    10021 gagctgttct acagcgacgc ggagaaggcc gcgcggctcc tggacatcac gctgacgaag
    10081 cgcggcaact ccgccggcga gccggtcgtc atggcgggcg tgcccttcca ctcggtcgaa
    10141 ggctacctcg cgcggctgat ccggcagggc gagtcggtgg cgatctgcga gcaggtcggc
    10201 gacgtcgcca ccgccaaggg accggtcgag cgcaaggtgg tgcgtgtggt cacgccgggc
    10261 accctcaccg acaccgagct gttgaacgac aaggccgaag ccctcctgct ggcggtgcac
    10321 cagggttcgc gccatcgcct tggcctggcc tggctcagcg tgacgcaagg cgcgctgcaa
    10381 ctggccgaat gcgcggccga cgaactggaa agctgggtcg tgcgcatcgc tccgagcgag
    10441 ctgctgttcg gcgccgacgt gacgacagcg ttcgagcagc ggctcaaggc gctgcgcttt
    10501 gccacgccgc tgtcgctcac gcaaagaccc gaatggcagt tcgaagcggg gctgggcctg
    10561 cgcaagctcc tggagcagct caatagcgcc tcgctggcgg cctggggcgc cgaagaactg
    10621 gtacacgccc atgcggcggc tgcggccttg ctgtcgtacg ccgagcacac tcagggccgg
    10681 gcgctgtcgc acgtgcaaag cctgcaggtg cagcgtgacg acgagctgat cgacctgccg
    10741 gtgaccacgc gccgcaacct ggaactggtg cagacgctgc gcggcgagga cgcgcccacg
    10801 ctcttttcgc tgctggacac ctgcatgacc ggcatgggca gccgcatgct caagggctgg
    10861 atgctgcagt cgcggcgcga ccgcggcgag gcgcggcagc ggcacgaagc cgtcgccgtg
    10921 ctgcgcgagc gtctgtggca ggtattgcgc gccgagctca agggctgcag cgacgtcgag
    10981 cgcatcacgg cccgcgtggc gctgcgccag gtgcgcccgc gcgaactggt ggggcttgcg
    11041 cagtcgctgc agaaatcggc acagctcgcg cggcagttgg ccgggcagga cgccttattg
    11101 gccgggatcg ccgccgacat ggacccgccg gcaggttgcg ccgggatgct ggcgcgcaca
    11161 ctgctggagg agccggcggc gctggcgcgc gacgggggcg tgatcgcttc aggcctggac
    11221 accgagctgg acgaactgcg cgccatccag gacaactgcg acggcttcct gctcgagctg
    11281 gaaacccgcg agcgctcgcg caccggcatc gccaacctgc gcgtgcagtt caacaaggtg
    11341 cacggcttct acatcgagat ctcgcaaggc cagctggaca aggtgccggg cgattaccgg
    11401 cgccggcaga cactgaagaa cgccgagcgc ttcatcacgc cggaactcaa ggccttcgag
    11461 gacaaggcgc tgtcagcgca ggaccgtgcc ctggcgcgcg agaaatggct gtacgagcaa
    11521 ctgctcgacc aactgcaggc cttcgtgccc gcgctcaccc ggctcgcgcg ggcgctggcg
    11581 tcactcgacg cgctctgtgc gctggccgag cgctcgctca cgctcggctg gtgccggccg
    11641 cacttcatga aggaaccggg catcgagatc ctgcagggcc ggcatccggt cgtccaggcg
    11701 cggctggccg aaaccagcgg cggcgcgttc atcgccaacg acacccgcct gggcccgaaa
    11761 cagcgcatgc agatcatcac cggccccaac atgggcggca agtcgaccta tatgcgccag
    11821 gtggcgctcg tctgcctgct cgcctcgatg ggttcatatg tcccggcggc cgaatgccgg
    11881 ctcgggccga tggacgcgat ccacacccgc atcggcgccg cggacgacct ggccaatgcg
    11941 caatccactt tcatgctgga gatgaccgag gcggcgcaga tcctgcacgc ggcgacaccg
    12001 cagagcctgg tgttgatgga cgagatcggc cgtgggactt cgactttcga cggcctggcg
    12061 ctggcctccg gcatcgccgc ccacctgcac gacaagaccc aggccttcac gctgttcgcc
    12121 acccactact tcgagttgac cgggttcccg gcccggcacc acgccgccct gaacgtgcac
    12181 gtgagcgccg ccgagtccgg ctcggacatc gtgttcctgc acgaaatcca gccgggcccg
    12241 gccagccgca gctacggcat ccaggtggcg cggctggcgg gcatgccggt ggccgtcgtc
    12301 aatcatgcca ggcacgcgtt ggccgccctg gaaacccagc agactctgag ccgggaacag
    12361 gtagacttgt tcgcagcccc gcctaccatc gaagcatcgg cacccagtgc ggtcgaagcg
    12421 gccgtggcgg ccctgaatcc cgacgcgttg agcccgcgcg aggccctgga cgcgctgtat
    12481 caactcaaga agctgggagg caagccatga cctactgcgt cggcatcaaa ctcaacgccg
    12541 ggctggtgtt cctgtccgat tcgcgcacca acgcgggcgt ggacaacatc agcactttcc
    12601 gcaagatgat catctacgag cgccccggcg accgcttcat ggtgctgctt tccgcgggca
    12661 atctgagcat cgcccagtcg gtgcgcgaaa tcctgcaagt cgaacagctc aaggagcacc
    12721 ccgacagcga ggtcatcaat atctggaacg ccaagagcat gttcgacgcg gcacgggtgc
    12781 tgggcaacgc ggtgcgccat gtgtacgacc gcgacgcgga ggcgctgaag aacgccggcg
    12841 tggagttcaa cgtccagctg atcttcggcg gccagatccg cggcgagagc atgcggctgt
    12901 tccaggtgta ttcggccggc aacttcatcg aggccacgcc cgagacgccc tacttccagg
    12961 tcggcgaata caagtacggc aagccggtgc tggaccgcgt gatcacgccc gacaccccgc
    13021 tggacgaagc ggccaaatgc gcgctggtat ccatggattc gaccatgaag tccaacctgt
    13081 ccgtcggccc gccgctggac ctggtcgtgt atgaagtgaa ccgtttcgag accgacaaga
    13141 tcgtctgcgt cgacatgcag aacccgtact accgcatgct gcacagcagc tggggccaga
    13201 agctgcgcga ggtgttcgac agcctcgaag atccggtctg ggacgatgcc cacaccgacg
    13261 tcccgctgaa ggtcgtggac gctcgcagca agccgctcaa gaaaatcacg cggccggatg
    13321 aaagactgat ctgagggcgc ggtgccgcgc ggcgcggcac tgctggccgg cgtcatggta
    13381 tccactaagc gccgccgctc gtttgtttca ctatcctctg gcagggcaga gcgcaggcgg
    13441 cgccctgcgc aacaacaacc cgactggaga caaactcatg aatcgccgac cgctctttcg
    13501 caccctggcg ggcctgctgt ccgccgccat cgtcagcgcg cccgcactgg ccgacaccat
    13561 caccctcagg gcttcgcacc agttccccgg cggcaaaggg gacgtgcgcg acgagatggt
    13621 gcagatcatc gcgcgcgagg ccgccgccgc caatgtcggg ctggagatca aggtcttccc
    13681 cggctccagc ctggtcaagg cgcaggagca gtggaaggcg atgcagaccg gccagatcga
    13741 catgacgtcg ttcccgctgg actacgcatc cgggttccat ccgcagttcg gcgccacgtt
    13801 gatgcccggc ctggtgaaaa accacgagca cgcggcgcgc atcaacgcgt cgcctttcat
    13861 gcaggacatc aaggcgatca tcgagcaggg cggcgtgaaa gtcctggccg acgcctggct
    13921 tgccggcgct ttcgccagca aggacaaatg catccgcaac cccgaggacg ccgcgggcct
    13981 gaaggtccgc tcggccggcg ccacgttctc gcagatgtgg gccggcgcgg gggcttccat
    14041 cgtgtcgatc ccctcaaacg aggtttacaa cgcactgcag caaggcgtgg cccaggccac
    14101 cgacacctcg tcgagcagct tcgtgtcgtt ccgcctgtac gagcagctca agtgcctcac
    14161 ggcgccgggg gacaacgcac tgtggttcat gtacgagccg gtgctgatct ccaagcgcag
    14221 ctgggacaag ctgaacgatg cgcagaagaa agccgtgatg gcgggctcga aaaaggccga
    14281 ggcatacttc tccgccgagt cgaagaagct ggacgacaaa atggtcgaca cgttcaagaa
    14341 aaataacgtg caggtcgtca cgatgaccga tgccgaggcc gaggcctggc gcgccgtcgc
    14401 ccggaaatcc tcgtacaaga tctttgcgga aaaagtgccc ggcggcaagg agctcatcga
    14461 taaggcgttg agtgtcaagt gacgcggcgc cgccgccggc ctgggtggcg gcggtgcatt
    14521 ccgtgtcccg gctgttcggg gtcgtcgccg ccggaatgat cctggtgtcg atcggggtgg
    14581 tctgccacat ggtcttcgtg cgagcggtgc tcgggcaatc gtccatctgg cagacagagt
    14641 tcgtcacctt ttcgctggtc gccgccacgt tcatcggcgc gccttacatc ctgctcacac
    14701 gaggccacgt cagtgtggac gtggttcccc tgatcgtgcg cgatcggacg cggcgcgtgc
    14761 tccacttcat cggctacgcg tcggcgctgg cgttctgcgc cttgttcctg tatgccgcgg
    14821 tgccgtggtg gtatgaaagc tggcagctgg gccagaccac gccttcgatc tggcgggcaa
    14881 ggctgtggat tccctatctg tcggtccccg tcggcctggc cttgctctgc ctggagctgc
    14941 tggccgagat ctggatggtc ctgtcgcggc gtgccttgcc gttcggcctg ggccgggacg
    15001 aggggccgtg agcaagcgga tcggccgcgc ccccgcctca tgagtcccat agccatcgga
    15061 ctgctggtct tcgtcgccac ctgcggcatc ctggccaccg gcctgccgat cgcgttcggc
    15121 ctgggcgtgg tggcgctgac gttcatggtg atcttcgacg gctggaacag cattcatttc
    15181 ctgccggaga ccgtcttctc gggcttgagc gatttcacgc tcgtatcgct gccgatgttc
    15241 gtcatcatgg gcgcggccgt cgcctcctcg cgcgccggca gcgacctgta cgaggcgctc
    15301 gcgcgctggc tgcaccgtgt gcccggctca ctggtgatct ccaacatcgg cgcctgtgcc
    15361 ttgttttccg cgctcaccgg ctcgtccccg gccacttgcg ccgccatcgg caagatgggc
    15421 atccccgaga tgcgccggcg cggctacgac gccgatctcg ccaccggcgc gatcgccgcc
    15481 ggcggcacgc tgggcattct catcccgccc agcatcacga tgatcctgta cggcatcgcg
    15541 tccgaaacct cgatcggccg cctgttcatc gcgggcatca ttcccggcct gctgctcacg
    15601 ggcctgttca tggcctgggc cctgttcctc agctggaagc gtggcacgcg gctggtggac
    15661 agcgggcgca tctacacctg gaaggaaaag ttcgagctgt tgccccgcct gttgccgttc
    15721 atcgccgtga tcgtgggcgt cgtgtacgcg ctgtacggcg gcatcgccac accgtccgag
    15781 gcggcgggcg tgggcgccat gctcacgctg gtgatggtgg ttgtgatcta ccgggtctgg
    15841 cggccggcgc acctctgggc catcctgcgc gacgggctgc gcgagtcggg catgctgctg
    15901 ctgatcatcg gcacctccat tctgttcggc tacatgctgt cgtcgctgca ggtgacgcag
    15961 tcggtcgccc agagcatcgg cgagcttcac gtcaaccggt ggatcgtgct cgccgcgatc
    16021 aacctgctgc tcctggtggc cggctgcttc ctgccgccgg ccgcgatcat cctgatgacc
    16081 acgcccatcc tgatgcccgt catctccgcc gccgggttcg acccgatctg gttcggggtg
    16141 atcctgacca tcaacatgga gctcggcctg atcactccgc cggtaggcct caacctgttc
    16201 gtgatcaacg gcatcacgcc ggacgtcaag ctgcccacca tcctgaaggg cgcgtttcct
    16261 ttcatgctgt gcatggtggc ggccatcgtg atcctttgcg tgttcccgga actcgccacc
    16321 tggctgccct cgcgcctgat gggttaggct tctggcctgg acgctgcgga ccgcggcatg
    16381 gcggcgcagc gcgtttcacc gaatgtccct catcatcttc tcccacggca acagctttcc
    16441 tgccgcgact tacggcgccc ttttcaagaa cctgcgcccg cgcggtttcg tcgtcagggc
    16501 cgtggagaaa ttcggccacg acccggacta ccccgtcacg gacaactggc gccacctggt
    16561 gcagcagctc catgacttca ccgcccgtga agtcgaccgg catggcgagc cggctttcct
    16621 ggtcggccat tcgctcggcg ggttcctcag cctgatgtgc gccgcgcgcc atccccagct
    16681 ggcgcgcggc gtgctgctgc tcgatgcgcc gctgctgggc ggctggaaag ccacggcgct
    16741 cggcgcgatc aagcgcagcc agctcgtggg ctcgatttcg cccggccgca tcagccgcca
    16801 gcgcaagcac cgctggccca gccgcgaggc cgccctcgaa cacttccgcc acaagaaagc
    16861 gttcgcgaac tgggacccac aggtgctgca cgattacgtg acgcactgca tgcacgacga
    16921 aggcggcgag cgcgtgctga gcttcgaccg cgacgtcgag accgcgatct acaacacctt
    16981 gccccacaac ctggaccgtt tgctggggcg ccatccgcca aagtgcccgg tggctttcat
    17041 cggcgggcgc cagtccgagg agatgaagcg ggtcggcatg cagatgacgc agcaagtcac
    17101 caagggccgg atcatgatga tcgacggcag ccacctgttc ccgatggaaa agccggttgc
    17161 cacggccgcg gcgatcgagg cggcactgcg gggcttcgat ttcccggttt gagccccaaa
    17221 gttgtcatcc gcactgttgc agtgcagcat tgggggcttc gtcctacagc caactccgcc
    17281 cggtctcaca agcccgccct ccatgacccg gcacattgct gggcatggat tcaacacaga
    17341 ccttctccca tccggtgcac gcaccgtcga tttcgctcct ggtcatggag cccttgcgtg
    17401 ccttgttcga tcactttgcg gggcgcatcg gctgcgaggc cctgccattg ggcgatgggc
    17461 atccggtggt cgtctatccg gggctggggg gcggcgccct caccacctcc cacctgcggc
    17521 gttttctctc cgactcgggc ttcacggtgc acgactggga aggcggggtg aacaccgggc
    17581 ccggcggcat cttcgatgat tggctggggg gactggagca gcgcgtgcgc ggcctgcacc
    17641 gcgagcatgg acgcaaggcc agtctggtcg gctggagcct gggcggcgtc tacgcgcgcg
    17701 aaatcgccaa acggtgtccc gaatccgtgc ggcaggtgat cacgctgggc acgcctttcg
    17761 ccgcgctggg tggcggcaac cacgcaaccg ccgtgtacaa gctgctcaat cgggatcaca
    17821 cgcaactgac gcccgaactc caggcacggc tgcgcgagtg cccgccggtg cccacgacgt
    17881 cgatcttcag caagaccgac gggatcgtgt cgtggcgcgg ctgcatcgag aaaaagtcgg
    17941 tgagttcgga gagcgtggaa gtcgccgcca gccatctggg catgggcacg cacccgcagg
    18001 tgctgcgcat cgtggcgaac cgcctcgcgc agcccgaagg ccggtggcgc gcgctcaggc
    18061 gcagcgagcg gctggggcgg ccgcgtctgg cccggggctg atcaaggggc ccatgtcacc
    18121 agtccgctcc aggccgtggc cagcaccacg atgccgaaag cgatccgata ccaggcgaat
    18181 ggcgtgaagc tatgggtgga aatgaagcgc agcagccagc gcacgcacat ccaggcgctg
    18241 atgaaagaga acaacaggcc gacggtgaag atcggcaggt cgtccaccgt catcagcgca
    18301 cgctccttgt acaggctgta cgcgcccgca ccgatcagcg ttggaatcgc caggtaaaac
    18361 gaaaaatccg tcgccgcccg gcgcgataaa cccagcagca tcgcgccaat gatggtggcg
    18421 ccgctgcggc tggtgccggg gatcatcgcg aggcactgca ccagccccac cttgagggcg
    18481 tctttccacg acatctggtc cacatcgtga atgcgcaccg ccgactgctg gcgcttctcg
    18541 gcccacagga tcaccagccc cccgaggatg aaggcgctgg ccaccacggc cggcgtgaac
    18601 aggttggcct ggatcgcctt gccgaacagc aggcccagca caactgccgg aaagaacgcg
    18661 atcagcacgt tcagcgaaaa ctgctgcgcc tgcctttgcg tgggcagggc caccagcgtc
    18721 tggcggatct tctgccagta gacgatgatc accgcgagga tcgcgccggt ctggatcgcg
    18781 atgtcgaaga ccttggcctt gtcgtcatga aaaccgagca aggcgccggc caggatgagg
    18841 tggcccgtgc tcgagaccgg aagaaattcg gtcagcccct cgaccacccc catcaccgcg
    18901 gccttggcca gcaacacgaa atccacgcac tctccttgaa taaggcccca cgcctggctg
    18961 ccctcaacgg ggcgattctt gtcttaaggg cacccggcga caagagaggc tggattatcg
    19021 ctggggcggt aacgaagccg aacacgccac cgcgaaatga ggcgatgccg gtgccgtccg
    19081 cgatgcccgg gcgcatgtcg agcgccttca tcgcccagca gccagacgtg taaggccgcc
    19141 gcaaatcgca ccggggcagc cgcgtttcgc gcccggtttc ctgcttttcg tcgcatggcg
    19201 ctcgcccgcc ggcggacgcg tcggcatatt ccacccatcg cacaagccag gaaaacgcat
    19261 gacgcaactc acaccgctca tcgccatcca catgacggcc gccctgctgg cggtggtcac
    19321 gggtccggtc gcgctctggg cgcgcaaggg cgccacgcag cggccgaagc tgcaccgatc
    19381 cttcggctat gcctgggtca ccctgatgct catcgcagcc ggctcggcct tctctattcg
    19441 cggcggcgag ctgcccaaca tcggcggctt cagccccatc cacctgttta ttccggtggt
    19501 gctgttcggc ctcgtcggcg ccttctggtt cctggccaaa ggcaacatcc gcgcgcaccg
    19561 caggaccatg ctgggcctgt acctcggcgc ttgcctgggg gccggggcgt ttgccctcct
    19621 gcccagccgg ctgctcgggc agttcctgct cggcctcgcc tgaacccgcc tattccaccc
    19681 cacaggagaa aaacaaatgc tgatccagat gcttatcgaa cgcccccaaa tgctgggccg
    19741 ggtcctgaac ggaacgccgc cctgggtctg gggcctgctg gccggcctcg tcttgctggg
    19801 attgagccag ggcagggcgc gaacggccgg cctggcgcgt atcgcgattc tgcccgtagc
    19861 gatgattgtg cttgcggtgt ggggcctgat gtcggctttc cgcggctcgc cccaactcgg
    19921 ttcggtgttg ctggcctggt ttgccgccgc cgccctgtcg ctggcagcca cggcgtcgat
    19981 ggcgccgccc gcaggcgcgc gttatgacgc tgtttcgcgg atgttcagcc tgcccggcag
    20041 ttgggtgccc atgctgctga tcctgggcat cttcctgacc aagtacatcg tgggcgtgga
    20101 gctcgcaatg caaccggccc tggcacatga tggccaatac agcctcgtgg ttagcgcgat
    20161 ctacggcctt ttcagcggca tcttcaccgg ccgcgccgtc cgcctgtggc gcctcgcctc
    20221 ccggcccgcg agcgccgcca ccgcgcaacc catcaacgcc tgaacacctc actcgaccaa
    20281 ggagaacctc atgaacgcac tgacccagct cgatgaacgt ggcctggaga agctggcgcg
    20341 caagcgcgcg ggggccaaac tggggtggta tatccacgcc ctcgtgtaca cctgtgtcaa
    20401 cctgctgctg gccatgctgt cggccgcgag cggcggcgac tgggccgtgt tcccggcgct
    20461 cggctggggc ataggcctgg ccgcgcacgg cctcgtcacg ttcgtcgtga ccggcggcgc
    20521 cggcctgcat gagcgcctgg tgcaacggga acgcaggcgc ctcacgctgc agcgcgaccc
    20581 gtggtgaggg gatcccgatc gtgacacgcc aggccgaaat ggcccgcgcc ttgctgcgca
    20641 acctgctcat cagcctgctt gccttgctgg gtgcctgggc gaatgcgggc atgggcccgg
    20701 cttggaccgc agcgccgtgc ccgccgcgca tgccgcgacc gcggatttct tccggcggca
    20761 ttttggtcac taaaatgagc ttgtcactgt cgcaatcccg caccatgaga acgctgtcct
    20821 ccgaagacct cgagcgcatc gcgcacaaac gcgccggcgc caagctcggc tggtatgtgc
    20881 atgccgcgct ctacatcgtg gtcaacctgt tcgtgttcgc gatttcgagc tacggcttcg
    20941 gcgagcggcc atggtccgta ttcccgctcc tgggctgggg cctgggcctg ggcctgcacg
    21001 gcatctcggt gttcatgctg ggtgccggcg gcgagttccg cgagcggctg gtgaagcagg
    21061 agcgtgaccg actgcgccgg caccaagacg gctcgtgaag cggccggaat gaagatcgac
    21121 tgggtcgtca agctgcagca cctgctgcag gtgctggcgt tttgcctggc ggtcgccacc
    21181 atccagtacg gatttcagcc cgatcggccc tacgcgccga acgtggtgca ctcgctcctg
    21241 atcggatcca ccatgtgggc catcatcgac ctcgggcgtc acctgatgcc ctcgagcgcc
    21301 gaaaccggct ggccgcaggg catcgccggc ctcgcgctgg tggtcggcgg tatcacggca
    21361 ggctatctcc tgggcaccac gatcgccgac cggctttgcg tctactacgg ctggtacccg
    21421 gcgggcacgc gcgacaacag cgcggcaaat tggcgcacct cgatcctcat caccgtgctg
    21481 gccggcagcg tgggcacctt ctacttctac agcgttaaca agagcgccta tctgaagcgc
    21541 aagatgaccg aggcgagcca gcacgcgacc gaggcccggc tgaagctgct ggaaacgcag
    21601 ctggagccgc acatgctgtt caacacgctg gccaacctgc gcgcgctgat agggtccgat
    21661 ccgaggcagg cgcaggagat gctggaccac atgatcgcct acctgcgcgc cacgctgagc
    21721 gcttcgcgca ccaccactca cagcctgcag gccgagttcg accggctgcg cgactacctg
    21781 gaactgatga ccgtgcgcat gggcccgcgg ctggactact cactggagtt gccgcccgag
    21841 ctggcgcagc atccggtgcc ggcattgctg ctgcagcccc tcgtcgaaaa cagcatccag
    21901 cacggcctgg aacccaaggt ggaaggcggc cgcgtcaccg tcagcgcccg gcgcgaagac
    21961 ggccggctgg tgctggaagt tgcggactcc ggcgtcgggc cttcgggtgt gacggccgac
    22021 ggcaagggct tcggcatgaa ccaggtgcgc gaacggctgg ccaccctgca cggcgcggcg
    22081 gcgcgcctgg acttcgcggc ggcgcccggc ggcggtgccc gcaccatcct gagtctgccc
    22141 ctgcaatcat gaacgccgcc ggcgccaccg ccctgatcgc cgaagacgag ccgctgctgg
    22201 cgcgcgagct gcaggccgaa ttggcccgcg cctggccgca actgcgcatc gtggcgacgg
    22261 tcggcgacgg cgcttcagcc gtggcccagg cactcgcgct caagcccgac gtgctgttct
    22321 tcgacatccg catgcccggg caaagcggac tggaggcggc ggtggaactg gccgacgagt
    22381 ggccgcagga gctgcccttc cccgcgctgg tgttcgtgac ggcctacgac cagtacgcgg
    22441 tgcaggcctt cgagaccgag gccgtggact acgtgctcaa gccggtgcag gcggcgcgcc
    22501 tgcaaaaaac cgtggcgaaa gtgcaggacg tgctggcgcg gcgcgcgcgc ggcacggtgg
    22561 acttcgacgc gacgctgggc cagctgcgca gcctgctcgg gtccgttgcg ccgacggcgc
    22621 ctgcggccgc cggcccgcca aacgagggtc cgctgaaaat gatccaggtc agcgtgggca
    22681 acagcatccg catggtgccg gtggaagaag tcttgtactt cgaggccgcg gacaagtatg
    22741 tgcgggtgct gaccgaaggc cacgagtacc tgatccgcac gccactgaag gaattgacgc
    22801 cgcaactcga cgccgggcgc ttctggcaag tgcaccgcgg caccgtcgtg cgagccgacg
    22861 ccatcgacag cgtcacgcgc gacgaagccg gcaaactgca cctgcagctt cgcggccgca
    22921 gcgaaaaact cgccgtcagc cgtctctaca cgcacctgtt caaggccatg tgaggaaagc
    22981 cgccacctgc cgaggcatca cgctccgccc ccgcaatagg cagttaggac tacctccaac
    23041 ggttgaggca aacgtctggc aaattttgtg ggcgccgtcc tacattcaag gcggcgggcc
    23101 cgaggtctac tacaagtcaa tcaccccgac tcctctgagt cccctcctcc tccggggtga
    23161 gctggtccgg ccctccgtcc cgcgactggc atgttgcccc caggcccaaa agcctggggg
    23221 ttcttttttg tccgcccaca aactgcagcg ccccaaaaaa acggccccga cgcttgcgcg
    23281 cggggcccag cttggagaaa gtggcttatt ttttaacgct gacccttgct aatggcggcc
    23341 gcggtgcttg cggtgtttat tggctttcca gtgaccgcgc ggcccgtagt cgtagtaata
    23401 gccggcgccg tagacgggcg cgggccggac gtaatagatg ggcgcgggac gcgcgtagta
    23461 ggcgggcgcg ggcacgaaga ccggcgctgg gcgcacatag tactgcgccg gcgggtagta
    23521 aaccggcgcg ggctgcaggt agaccggggg gtagtacaca ggcgcattgg acaccccgat
    23581 gctcacaccg ggtgcaacat gggcgccgat cgagtagtac acgtcgctgc gtgcctgggc
    23641 caccgacgcc gtgcctgctg ccgccagcgc cagggcagcg gcagccacct tgacgtaaac
    23701 ggaacggctg gagttcatgt ggtttctcct gttagttggg gccaacaacg ccccttgccg
    23761 ttatttaaac gcacctccag cccgaacggc tagcctctgc acagtcaaag gcgttgccag
    23821 aagtaactca tccattcggg agataaggcg aggaatatgt tgtatcaacc cataaaatgg
    23881 tttctatatg acctccgctg ccgataaaga acccctaaaa cccagcaatt tcctgcgcca
    23941 ggtgatcgag cgcgacctgg agcaaggaac atatgcacaa cgtagatggg gcggcagccc
    24001 cggtgatgca gcgcaccata tccagggaca acccgacccg gccaggatcc gcacccgctt
    24061 tccgcccgag cccaacggtt acctgcacgt cggccacgcc aagagcatct gcctgaattt
    24121 cggcctggcg cgcgactatg gcggcgtctg ccacatgcgc ttcgacgaca ccaaccccga
    24181 aaaggaagac ctggagtacg tcaacagcat cctggaggcg gtgacgtggc tgggcttttc
    24241 atgggaggcc aacggcacca gccactatta ccacgccagc gactacttcg acttcatgta
    24301 ccgcgcggcc gagtacctga tcgaggccgg cctggcctat gtggacgaac agacgcccga
    24361 agagatgcgt gcccatcgcg gcgacttcaa caagcccggc accgacagcc ccttccggtc
    24421 ccgctcgatc gacgagaacc tgcagcgttt tcgcgacatg cagggcggca agctggccga
    24481 cggcgccgcc gtgctgcgcg ccaagatcga catggccagc cgcaacatca acctgcgcga
    24541 cccggcgatc taccgcatcc gccgcgccac gcaccacaac accggcgaca agtggtgcat
    24601 ctacccgatg tacacctatg cgcacccgat cgaggatgcg ctggagaaca tcacccacag
    24661 catctgcacg ctggagtttg aggaccagcg gccgttctac gactggctgt tggaccggct
    24721 cggcgaaggc ggcctcacaa accagccgca cccgcgccaa tatgaattcg cgcggctgaa
    24781 cctgacgtac gtgatcacca gcaagcgaaa gctgaagcag ctggtggacg aaaaacatgt
    24841 gagcggctgg gacgacccgc gcatgcccac catcgtgggc ctgcgccggc gcggctacac
    24901 gcccgagagc atccagctgt tcgccgagcg catcggcgtc accaagagcg acagctggat
    24961 cgattacgcg acgctggagc aggcgctgcg cgacgacctg gacccgaagg cgcccagggc
    25021 gatggcggtg ctggatccgg tgaagctagt gatcacgaac tggggccagg tgatgggaag
    25081 cgatgacaag cttgacgact gctcggcgcc gctgcatccg aacaaccccg agatggtggg
    25141 ccggcgcgat ttcaagttcg gcagggagct ctggatcgag cgcaccgatt atgaggaagt
    25201 cccgcccaag ggctacaacc ggctgtaccc cggcaacaag gtgcggctga agtacggtca
    25261 cgtgatcgag tgcaagggcg cggtgaagga caaggacgat cgcgtcaccg tggtgcgggc
    25321 cgaactgatc cccgacacca agagcggcac g
//
LOCUS       DASWIY010000233         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_878899, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000233 DASWIY010000000
VERSION     DASWIY010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878899"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..1632)
                     /locus_tag="VGA59_08635"
     CDS             complement(352..1632)
                     /locus_tag="VGA59_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX9719784.1"
                     /translation="MVTQGVLPFKLEVTRDAMTPHAGLALFGEFLPALGLARWLDEAL
                     PEPGSARGYAPSAFVVPLVLMLHGGGRSLEDLRQVRQDEALRELLHLEELPSADATGD
                     WLRRMGAGPGLAALGAVHRRVLAWGLRRMKQREFTLDIDATQIVAEKQEALRTYKGEV
                     GYMPIVGHLAENGLIVGEEFRAGNASPNARNAEFIGHCAAQMPKGTRIAHLRADNAAY
                     QAEIFNACEAQGRSFAIGAPLDAAVLAAIGAIAEEDWRPYQNGAITETVHSMDHTRHA
                     FRLVVIRRPVQRTLLGEEDPAERYRAIASNRQESAEETLAWYNARGEHSENRIKELKN
                     GFAMERMPCGTLEANAVFFRIGALAYNLFCLFKRLALPQSWRRHQVQTLRWRLYQVAG
                     KVVTHAGAVILKVALEMFALFAEIRTRCRELACA"
     gene            complement(1854..2306)
                     /locus_tag="VGA59_08640"
     CDS             complement(1854..2306)
                     /locus_tag="VGA59_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719785.1"
                     /translation="METNIAVPKGGVIHPSRPVSRRTAEPILYLADLMASADNEPVPR
                     ELRIVDMVADAVGMPTFRHQPWFRQMNEAAAVERLNTELAKRAALVVLALVLKADVKR
                     KPSEHAFFTRIREALGAEPITVPVDIEEHRKLALEYFVDAATRRAPSR"
     gene            complement(2529..2954)
                     /locus_tag="VGA59_08645"
     CDS             complement(2529..2954)
                     /locus_tag="VGA59_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719786.1"
                     /translation="MNQTGQATLRHSERPTHAVPRKYIEPIMYLAERMSMQDKLVPAP
                     GRRVVDQLAEALHMKDFRRQPWFRSMSDQHACELIDLETVKRATLIVIALVMKGDTAR
                     GDAAKAYFTRVRELMGVDSIAVPADMDAHMDLTLRHLVG"
BASE COUNT          449 a         1005 c         1013 g          524 t
ORIGIN      
        1 gcgccgcttg gcgcgggtgc gccacggcgc tgccccccga ccgggtacct ggatacaagc
       61 tttatgatag cagcgccggc cacgccgctc cgtgacgtgg tcacgctccc cgccgagcag
      121 ggatggggcc cttcgcgccc caaatcccgc gatgagctgg gacaggcggg atggagccat
      181 cgggctggga ggctttgagg ccgcccgggt ggttcagagg cggtctgtta gcgcatgttg
      241 gtctccggat cgggtggcgt cgagggcccg gtgtcgggtt ttatcgggcg cagggcacag
      301 atgtgcgggg cccggcgcgc gggcggagga aggatggcgg tgtggagccg gtcaggcaca
      361 ggccagctcg cggcagcggg tgcggatttc ggcgaacagg gcgaacatct ccagggcgac
      421 cttgaggatc actgcgccgg catgggtcac caccttgccg gcaacctggt agagccgcca
      481 gcgcagggtc tgcacctggt ggcggcgcca gctttgaggc agcgcgaggc gcttgaacag
      541 gcagaacagg ttgtaggcca gcgccccgat gcggaagaag accgcgttgg cctccagggt
      601 gccgcaaggc atgcgctcca tggcgaagcc gttcttcagc tccttgatgc ggttctccga
      661 gtgctcgccg cgggcgttgt accaggcgag ggtctcctcg gcgctctctt ggcggttgct
      721 ggcaatggcg cggtagcgct cggcggggtc ttcctcgccg agcagcgtgc gttgcaccgg
      781 ccggcggatg accaccaggc ggaaggcgtg gcgggtatga tccatgctgt gcaccgtctc
      841 ggtgatggcg ccgttctggt agggccgcca gtcctcctcc gcaatggccc cgatcgccgc
      901 cagcaccgcg gcgtccagcg gagccccgat ggcgaagctg cggccctgtg cctcgcacgc
      961 gttgaagatt tccgcctggt aggccgcgtt gtcggcacgc aggtgcgcga tgcgcgtgcc
     1021 tttgggcatc tgtgcggcgc agtggccgat gaactcggcg ttgcgcgcgt tgggcgaggc
     1081 gttgccggcc cggaattcct cccccacgat caagccgttt tccgccaggt ggcccacgat
     1141 gggcatgtag cccacctcgc ccttgtaggt gcgcagcgct tcctgctttt cggccacgat
     1201 ctgcgtggcg tcgatgtcca gcgtaaactc gcgctgtttc atccggcgca gtccccaggc
     1261 gagcacccgg cggtgcaccg cgcccagcgc tgccagaccc ggcccggcgc ccatccgccg
     1321 cagccagtcc cccgtcgcat ccgcactcgg caactcctcc aggtgcaaca gctcgcgcaa
     1381 ggcctcgtcc tggcgcactt ggcgcaaatc ctccagcgaa cgcccgccgc cgtgcagcat
     1441 cagcaccagc ggcaccacaa aggccgacgg agcgtagccc cgcgcgctgc ccggctccgg
     1501 cagcgcctcg tccagccacc gcgccagccc gagcgcgggc aggaactcgc cgaacagcgc
     1561 cagccccgca tgcggcgtca tcgcgtccct cgtcacttcc agcttgaacg ggagcacccc
     1621 ttgtgtgacc atccctgtca ccccgttggt ggtggctcaa gtccttcttt cacaccttga
     1681 acctaccaga accagcgggg ttttctcctt cgtctccttt tccatcgcgg gatttgggac
     1741 gcacgcaccc gtcgcggtgc ggcggcaagc ccgcggttgg cgatgaacgc ggtaaggtcc
     1801 gggccggccc actggcggga ccgctccggg cgcagaagtt ccgggcgcgg aaattagcgg
     1861 gaaggggcgc gtcgcgtcgc agcgtccacg aagtattcca gcgccagctt cctgtgctcc
     1921 tcgatgtcca ccggcacagt gatcggctcg gcgcccaagg cctcgcggat gcgcgtgaag
     1981 aacgcatgct cagagggctt gcgcttcacg tctgccttga gcaccagcgc cagcaccacc
     2041 aacgcggcac gcttggccag ttcggtgttg agccgctcga cggcagcggc ttcgttcatc
     2101 tgacggaacc agggctgatg ccgaaacgtg ggcatgccca ccgcatcggc gaccatgtct
     2161 acgatgcgca gttcccgggg caccggctcg ttgtcggcgc tcgccataag gtcggccagg
     2221 tacaggatcg gctcagccgt gcggcgcgag acaggccggg agggatggat taccccaccc
     2281 ttgggcacag cgatgttggt ttccatgctg ccacctgcaa atgcgaagtg gtctcgatga
     2341 gtttcgtttt tttaagcgca aatcgcaggc cgatgcaagc ggattccgag acgctgcacg
     2401 gattccggaa ggcgccggcg gggcctacct gccaagccgc caggcaaccc tggacccggc
     2461 tctcttgcgg ccaggcaggc ccgcaaattc tgggcccgct cctgctgtgc ggcgccggga
     2521 gcgtttgctc agccgaccag gtggcgcagg gtgaggtcca tgtgcgcgtc catatctgcg
     2581 ggcacggcaa tcgagtcgac gcccatcaac tcacgcactc tggtgaagta ggccttggca
     2641 gcatcgccgc gcgccgtgtc ccccttcatc accagcgcga tgacaatgag tgtcgcgcgc
     2701 ttgaccgtct ccagatcgat cagctcgcag gcgtgctggt cgctcatgga gcggaaccag
     2761 ggctggcgcc ggaagtcctt catgtgcagc gcctcggcga gctgatccac cacgcgcctg
     2821 ccgggagctg gaacgagctt gtcctgcatg ctcatccgct ccgcaaggta catgatgggt
     2881 tcgatgtact tgcgaggtac ggcatgggtc ggccgttcgc tgtgccgcag cgtggcttgg
     2941 cctgtctggt tcatggggcc tccttgattg cgctcctctc cgtggcacag g
//
LOCUS       DASWIY010000234         6871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3454943, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000234 DASWIY010000000
VERSION     DASWIY010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3454943"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1020)
                     /gene="sbmA"
                     /locus_tag="VGA59_08650"
     CDS             complement(13..1020)
                     /gene="sbmA"
                     /locus_tag="VGA59_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HEX9719787.1"
                     /translation="MFRSFFFSRRWLPWSVLGTLLILFATWYRVQLDVQINEWFGTFY
                     DLIQKALGKPGTITMADYWAEIATFFRIAMIYVTIAVVLDFFVKHYIFRWRTAMNDYY
                     MEHWHKLRHVEGAAQRVQEDTMRFASIMEGLGIDFMRSLMVLAAFLPILATLSAKVTE
                     LPYFGPVTYSLVWVAIGFALAGTVLLAIVGIKLPGLQFQNQRVEAAYRKELVYGEDDA
                     NRASPPVAGKLFAQVRLNYFRLFFHYLYFDVAKWSYLQLGVLVPYIALAPTLVAGVVT
                     LGVMQQIVRAFGRVESAFQFLVLSWSTVIELISVYKRLRAFELQIREGGGAAPMAIEG
                     A"
     gene            2004..2273
                     /locus_tag="VGA59_08655"
     CDS             2004..2273
                     /locus_tag="VGA59_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719788.1"
                     /translation="MALAPALALAPALALAPALARAPALALAAPAPALALAVAALAPA
                     VAVAVAALALALALAAARAAALAAALAATLPGRAGRVEMAAAVSA"
     gene            complement(2560..5145)
                     /locus_tag="VGA59_08660"
     CDS             complement(2560..5145)
                     /locus_tag="VGA59_08660"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEX9719789.1"
                     /translation="MLQAYVTHVAERAALGIPPLPLSAQQTADLIELLKNPSPGEAEF
                     LLNLITHRVPAGVDDAAKVKASYLAAVAHGSEKCEVISREMATELLGTMLGGYNIGPL
                     IDLLDDAQVGGIAAEGLKKTLLMFDQFHDVQETAGKGNRNAQAVLQSWADAEWFTSRP
                     EVPQSMKVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRPGITPEEEGKRG
                     PVKFIEELRSRGNLVAYVGDVVGTGSSRKSATNSVLWFTGEDIPYVPNKRFGGVCLGS
                     KIAPIFYNTMEDAGALPIELDVSRMEMGDVVELRPYEGRALKNGAVIAEFKVKSEVLF
                     DEVRAGGRIPLIVGRGLTAKAREALGLAPSTLFRLPHDPTDTGKGYTLAQKMVGRACG
                     LPEGKGVRPGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSSDLVMQSFCHTAAYP
                     KPVDVKMHHELPEFISNRGGISLRPGDGIIHSWLNRMLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFKGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKQGKKNIFSGRILEIEGLPQLKVEQAFELSDASAERSAAGCTVHLDKAPIIEYINSN
                     ITMLKWMIANGYADARTIDRRIKAMQAWLAGPQLLKADANADYAAVIEIDLADIHEPI
                     LACPNDPDDVKTLSEVAGTKIDEVFIGSCMTNIGHFRAASKLLEGKRDIPVRLWMAPP
                     TKMDAHQLTEEGHYGVLGSAGARMEMPGCSLCMGNQAQVREGATVVSTSTRNFPNRLG
                     KNSNVYLGSAELASICSKLGRIPTKEEYMVDMGVLTAASDKVYQYLNFDRIAEFRDAA
                     DPLPA"
     gene            complement(5188..5766)
                     /locus_tag="VGA59_08665"
     CDS             complement(5188..5766)
                     /locus_tag="VGA59_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719790.1"
                     /translation="MKVLLVTLLIAATPALALAQSTAPAKPAAKSPAKKPVPKKSRKL
                     APAAQKAVEEVTPIEDDPSIVLSEADLEVARKVHVGDIPCELGATVKVMPMKREGMFS
                     VMTGGHRFRMHPVESRTGAIRLEDPQRGAMWLQLGNKSMLMSQKLGQRLADECQSPAQ
                     VTYADMLKKNPLPSILEPLPGPQSTSPKAGPN"
     gene            complement(5763..6773)
                     /locus_tag="VGA59_08670"
     CDS             complement(5763..6773)
                     /locus_tag="VGA59_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX9719791.1"
                     /translation="MHPREVLLGAQAQTGFLPVCDHYSGVEARMRKSLALQAEMCEEF
                     GACVFDVTLDCEDGAPVGGEAEHAALVVALASLASDKARVAVRVHPVGHPAFEQDVAI
                     IAGKAGNRLCHIMIPKVESVQDVQAACKALDAAGASQLQLQVLIESPAAVHRAFDIAA
                     HPRVQSLSFGLMDFVAAHGGAIPSEGMGIKGQFTHPLVVRAKLEIASACHAWGKVPSH
                     SVVTEFKDAKALQAAARKAAREFGYTRMWSIHPDQIRPILEAFAPSAHEIEVATKIIA
                     AAARVDWAPISFEGKLEDRASYRYYWQVLERAHRTGRKLPQELQGLMEPPASLNPRRQ
                     VV"
BASE COUNT         1130 a         2232 c         2277 g         1232 t
ORIGIN      
        1 gccggtgacc cgctaggcgc cttcgatggc catcggcgcg gcccctccgc cttcgcggat
       61 ctgcaactcg aaggcacgca gccgcttgta cacggagatc agttcgatca ccgtgctcca
      121 gctcagaacc aggaattgga acgcgctttc cacgcggccg aacgcgcgca cgatctgctg
      181 catcacgccg agggtgacga cccctgctac cagcgtcggg gcgagggcaa tgtaggggac
      241 cagcacgccg agctgcaggt acgaccattt cgccacgtcg aaatagaggt agtggaagaa
      301 cagccggaaa tagttgaggc gcacctgggc gaacagcttg cccgccaccg gcggtgacgc
      361 gcggttcgca tcgtcctcgc catatacgag ttccttgcgg taggccgcct cgacgcgctg
      421 attctggaat tgcaagccgg gcagcttgat gcccacgatc gcaagcaaca ccgtgccggc
      481 cagcgcgaag ccgatcgcca cccagaccag cgaatacgtc accggcccga aatagggaag
      541 ctcggtgact ttcgccgaaa gcgtcgccag gatgggcagg aacgccgcca gcaccatcaa
      601 gctgcgcatg aagtcgatac cgaggccttc catgatgctg gcaaagcgca tggtgtcctc
      661 ctgcacacgc tgtgcggcac cttcgacatg ccgcagcttg tgccaatgtt ccatgtagta
      721 gtcgttcatc gccgtgcgcc accggaagat gtagtgcttg acgaagaaat ccagcaccac
      781 cgcgatggtc acgtagatca tggcgatacg aaagaacgtt gcgatctccg cccagtagtc
      841 ggccatggtg atggtgcccg gcttgcccag tgctttctgg atcagatcgt agaaagtgcc
      901 gaaccattcg ttgatctgta cgtccagttg gacccggtac caggtcgcga aaaggatgag
      961 cagcgtgccc agcacggacc acggcaacca gcggcgcgaa aaaaagaaag agcggaacat
     1021 cgcgatacac cttgctgttt tggttgcgcc catggtagcc ggtgccctcg cccgcgcgta
     1081 atggatgtgt ctgctttctc ttacacgagg aattgatttg acgcacaaga aacgtgggcc
     1141 gttaccgaca acgctgggca gtggcgttgg cgaccatgag ggcatatgcg gcacaacaat
     1201 aaatgcagaa aggacaaccc aatgatcgtt tctcgatccg aagtgttctt ctgctcatcc
     1261 gaaggaatcg ggtatgcccc gcgaggcatg gcgctaggcc aaggcggcgt tgccgcattc
     1321 aacgccgcgg ttggtttcaa ctggacagca aacgtcacat accgacgagc cgagcgcggc
     1381 acccgcaagg ccacgccgcc cccacacgga gcaccgatca tcgacgaacg tccgcacagg
     1441 cgccggcgat tgaagcggcg caccgattcc ttcttgcgcg acaccggccc gccgccataa
     1501 caaggccgga ccaccccttt actccaacct cgagagatgc atcatgaaat cactgaacag
     1561 aaagtttggt ctgacggtcc tggccgccgc acttgccggc gtatttggag tcatctggtc
     1621 gcagcccggc agcgccgcgg gtggcggcgc tagtgccggc gccagcgccg gcgccggggg
     1681 tggcggcggt ggtggcgttg gtgccggcgg cgtcggcggt ggcggcggct ccggcggtgg
     1741 cgtgggcggt ggctccggcg gtggctccgg tggcggtggc tccggtggcg gtggctccgg
     1801 cggtagcgtt agcggcagtc caggcggggc agtcgacggt aatcccgatg tcggcggcgg
     1861 ccccggcggt gcgcctgccg gtaacgcagg cggcagcggt ggcgccaacg tcgggactgg
     1921 caacagcgga gtcggcgttg gaaatacggg tctcgggaat cagggcaccg gtcctaacgc
     1981 cactaccgcg ggcaacgctt gctgtggcac tggcaccggc actggcactg gcaccggcac
     2041 tggcactggc accggcactg gcacgggcac cggcactggc actggcggca ccggcaccgg
     2101 cactggcact ggcggtggcg gcactggcac cggcggtggc ggtggcggtg gcggcactgg
     2161 cactggcact ggcactggcg gcggctcggg cggcagcgtt ggcggcggca ttggcggcga
     2221 cactgccggg ccgggcgggc cgagtggaga tggcggcggc ggtgtcggca tagcggttat
     2281 ccggcccaca acaccgcagc ctacattggc cctggagccg agagttgaag cacctgctgt
     2341 ggcacaacag caggccccgg cgccccaagg cgcaagcgcg gccgctcccg ccccggcacc
     2401 ggctgctcca gtcgtagcag ccgcgccgag cacaacgacc atgggtgcgg caccgaccga
     2461 gacgccggtg cgcaagccca ggcctgatcg caactgatcg cgcgctccag gaaaagcggc
     2521 cccgccaggg gccgcttttt catgccttcg ggcaaccggt catgcaggca gtgggtccgc
     2581 cgcgtccctg aactccgcga tccggtcgaa gttcaggtac tggtagacct tgtcgctggc
     2641 ggccgtgagc acgcccatgt cgaccatgta ctcttccttc gtcggtatcc tgccgagctt
     2701 ggaacagatc gacgccagct cggcgctgcc gaggtagacg ttgctgttct tgcccagccg
     2761 gttggggaag ttgcgagtgg aggtggaaac gaccgtggcg ccttcccgca cctgcgcctg
     2821 gttgcccatg cacagcgagc agcccggcat ctccatgcgc gcgcccgcac tgcccagcac
     2881 cccgtaatgc ccttcctcgg tcaactggtg ggcatccatc ttcgtcggcg gcgccatcca
     2941 cagccgcacc ggaatgtcgc gcttgccttc cagcagcttg gacgccgcgc ggaagtggcc
     3001 gatgttggtc atgcacgagc cgatgaacac ctcatcgatc ttcgtgcccg ccacctcgga
     3061 caaggtcttc acgtcgtcgg ggtcgttcgg acaggccagg atcggttcgt ggatgtcggc
     3121 caggtcgatc tcgatcactg cggcgtagtc cgcgttggcg tcggccttca gcagctgcgg
     3181 gccggccagc caggcctgca tcgccttgat gcggcgatcg atggtgcgcg catcggcgta
     3241 gccgttggcg atcatccact tgagcatcgt gatgttgctg ttgatgtatt cgatgatggg
     3301 ggccttgtcc aggtgcaccg tgcagccggc ggccgagcgt tccgccgagg cgtcgctcag
     3361 ctcgaacgcc tgctccacct tgagctgagg gaggccctcg atctcgagga tgcggccgga
     3421 gaagatgttc ttcttgccct gtttcgcgac cgtcaaaagg ccctgcttga tggcgtacag
     3481 cggaatcgcg ttgaccaggt cgcgcagcgt cacccccggt tgcagtttgc ctttgaagcg
     3541 cacaagcacg ctctcgggca tgtccagcgg catgacgccg gtggcggccg cgaaagccac
     3601 caggcccgag cccgccggga agctgatgcc gatgggaaag cgcgtgtggc tgtcgcctcc
     3661 ggtgcctacc gtgtcgggca gcagcatgcg attgagccag ctgtggatga tgccgtcgcc
     3721 ggggcgcagg ctgatgccgc cgcgattgga gatgaactcg ggcagctcgt ggtgcatctt
     3781 cacgtccacg ggcttcgggt acgccgccgt gtggcagaaa ctctgcatga ccaggtccga
     3841 gctgaagccc aggcaggcga ggtctttgag ctcgtcgcgc gtcatcggtc cggtcgtgtc
     3901 ctgcgaaccg acggaggtca tcttcggctc gcagtaggtg cctggccgga cgcccttgcc
     3961 ttcgggcagg ccgcaggcgc gccccaccat cttctgcgca agggtgtacc ccttgccggt
     4021 gtcggtgggg tcatgcggca gccggaacag cgttgacggc gcaaggccca gcgcctcgcg
     4081 cgccttggcc gtcagcccgc ggccgacgat cagagggatg cggccaccgg cgcgcacttc
     4141 gtcgaacagg acctcgctct tgaccttgaa ctcggcgatc accgcgccgt ttttcagcgc
     4201 cctgccttcg taggggcgca gttcgaccac gtcgcccatc tccatcctcg agacgtccag
     4261 ctcgatcggc agggccccgg cgtcttccat cgtgttgtag aagatcgggg cgatcttgga
     4321 gcccaggcac acgccgccga agcgcttgtt cggcacgtag gggatgtcct cgccggtgaa
     4381 ccacagcacc gagttggtcg ccgacttgcg gctggaaccg gtgcccacca cgtcgcccac
     4441 gtaggccacg aggttgcccc gagagcgcag ctcctcgatg aacttcaccg ggccgcgctt
     4501 gccttcttcc tcgggcgtga tgcccggccg cgggtttttc agcatggcca gcgcatgcag
     4561 cggaatgtcg gggcggctcc aggcgtctgg cgcgggcgag aggtcgtccg tattggtctc
     4621 gcccgtcact ttgaacacgg tcaccttcat gctctgcggc acttcggggc ggctggtgaa
     4681 ccactcggca tcggcccagc tttgcagcac tgcttgcgcg ttcctgttgc ctttgccggc
     4741 cgtttcctgg acgtcgtgga actggtcgaa catcagcagc gtctttttca ggccttcagc
     4801 ggcgatgccc cccacttgcg cgtcatccag caggtcgatc agcggaccga tgttgtagcc
     4861 gcccagcatg gtgcccagca gttcggtcgc catctcgcgg ctgatgacct cgcacttttc
     4921 gctgccgtgc gcgaccgccg ccaggtagct ggccttgacc ttggccgcgt cgtccacgcc
     4981 ggccggcacg cgatgcgtga tcaggttgag gaggaactcg gcttcgcccg gggacggatt
     5041 cttcagcagc tcgatcaggt cggcggtctg ctgcgccgac agcggcagcg gggggatgcc
     5101 gagcgcggcg cgttcggcga catgggttac ataggcttga agcatttctt ctccgttcaa
     5161 atcagcaaaa tcgggggcgc acgggcccta attgggaccc gccttcggcg aagtggactg
     5221 cggtcccggc aagggctcca ggatgctcgg cagggggttc ttcttcagca tgtcggcata
     5281 ggtcacctgc gccgggctct ggcactcgtc ggcgaggcgc tgaccgagct tctggctcat
     5341 caacatcgac ttgtttccca gttgcagcca catcgcgccg cgctgcggat cctccaggcg
     5401 aatggcgccg gtgcggcttt ccacgggatg catgcggaag cggtggcccc cggtcatgac
     5461 cgagaacatg ccctcgcgct tcatcggcat gaccttgacg gtcgcgccca gctcgcacgg
     5521 gatgtcgccc acatggacct ttctggcgac ctccagatcg gcttcgctga gcacgatact
     5581 cggatcgtcc tcgatcggcg tgacctcctc gacggccttt tgcgccgccg gcgcgagctt
     5641 gcgggatttc ttgggcactg gttttttggc gggcgatttc gcggccggct tggccggcgc
     5701 ggtggattgt gcgagcgcca gcgcgggcgt tgccgcgatg agcagagtga cgagaaggac
     5761 tttcatacaa cttgtctccg tggatttaac gaggcagggg gttccatcaa gccctgcaat
     5821 tcttgcggca gcttccttcc ggtccggtgc gccctctcca gcacctgcca gtagtagcgg
     5881 tagcttgccc tgtcctccag cttgccttcg aaactgatgg gagcccaatc gacccgggcc
     5941 gcagcagcta ttattttggt agcaacctcg atctcatgcg cgctgggagc gaatgcttcc
     6001 aggatcggcc ggatctggtc cggatggatg ctccacatcc gcgtgtagcc gaactcccgc
     6061 gcggcttttc gcgccgcggc ttgaagggcc ttggcgtcct tgaattcggt caccacgctg
     6121 tgcgaaggca ccttgcccca ggcatggcac gccgaggcga tctcgagctt cgcgcgcacc
     6181 accagcggat gcgtgaactg gcccttgatg cccatgccct ccgacgggat ggcgccgcca
     6241 tgggccgcaa cgaaatccat cagcccgaag ctgagcgact gcacacgcgg atgcgcggcg
     6301 atgtcgaacg cgcggtgcac cgccgccggg gattcgatca gcacctgcag ctgcagctgg
     6361 ctcgcaccgg ccgcgtcgag cgccttgcag gccgcctgga catcctgcac cgactcgacc
     6421 ttggggatca tgatgtggca cagcctgttg cccgccttgc cggcgatgat ggcgacgtcc
     6481 tgctcgaacg ccggatggcc gacggggtgg acgcgcaccg cgactcgcgc cttgtcgctg
     6541 gccaacgacg cgagcgccac caccagagcc gcatgctcgg cctcgcctcc gaccggcgcg
     6601 ccgtcctcgc agtcgagcgt cacgtcgaag acgcaggcgc cgaactcctc gcacatctcg
     6661 gcctgcagcg ccaggctctt gcgcatcctc gcctcgacgc cgctgtaatg gtcgcaaacc
     6721 ggaaggaagc ctgtttgagc ctgggcgccg aggagtactt ctcgagggtg cattcttact
     6781 gctaggggct aggggctacg gattagggct cgggcagggg aacagggatt ggcgattggc
     6841 gcggggggac cggtgctcgg ccggggactt g
//
LOCUS       DASWIY010000235         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3455422, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000235 DASWIY010000000
VERSION     DASWIY010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3455422"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VGA59_08675"
     CDS             complement(<1..477)
                     /locus_tag="VGA59_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9719792.1"
                     /translation="MISTANITMQFGAKPLFENVSVKFGGGNRYGLIGANGCGKSTFM
                     KILGDDLEPSGGQVMLEPNVRLGKLRQDQFAYEEFSVIDTVIMGHEELWAVKAERDRI
                     YSLPEMSEDDGMAVAELETDFAEMDGYTAESRAGELLMGLGIPLAQHFGPMSEVAPG"
     gene            complement(827..1105)
                     /locus_tag="VGA59_08680"
     CDS             complement(827..1105)
                     /locus_tag="VGA59_08680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004934175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9719793.1"
                     /translation="MAKAMARHILVKTEAQAAALKKRIAAGEAFDLLARQYSTCPSGK
                     RGGDLGKVRPGQMVRAIDQVIFNKPLREVHGPVKSQFGYHLVQVFYRD"
     gene            1254..>1940
                     /locus_tag="VGA59_08685"
     CDS             1254..>1940
                     /locus_tag="VGA59_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX9719794.1"
                     /translation="MDIHSMLKILASQDGSDLYLSTGAPPCAKFNGVLKALSQEPLKP
                     GEVAAIADAVMDVAQREEFERELEMNLAISLAGVGRFRINIFKQRNEVSIVARNIKLD
                     IPKFEDLKLPEVLLSTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
                     PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFA
                     DTGHLAISTLH"
BASE COUNT          366 a          590 c          604 g          380 t
ORIGIN      
        1 accgggggcg acttcgctca tcgggccgaa atgctgggcc agcggaatgc cgaggcccat
       61 cagcagctcg ccggcgcggg attcggcggt gtaaccgtcc atttcggcga aatcggtttc
      121 cagctcggcc acggccatgc cgtcgtcttc gctcatttcc ggcaaggaat agatgcggtc
      181 gcgctcggct ttgacggccc agagttcttc atggcccatg atcacggtat cgatcacgct
      241 gaactcttcg taggcgaact gatcctggcg cagtttgccc aggcgcacgt tcggctccag
      301 catcacctga ccaccgctcg gctccaggtc gtcaccgaga attttcatga aggtcgactt
      361 gccgcagccg ttcgccccga tcaggccgta gcggttgccg ccgccgaact tgacggaaac
      421 gttctcgaac aggggtttgg ccccgaattg catggtgata ttggcggtgg agatcacgaa
      481 tcgttgtcca gaaagcgagc gcgtgagcgc gaaaggggca agattgtaca gctttctggc
      541 ccggcaaggc agccgggagg acggatgggg tggttttgcc gggcgctctg agggtgtgaa
      601 aatatcgagc gccgccgcag cgggcgtttt ggcgatccgt agggtcactc gccgcaggca
      661 gtgcaccgtg gttcagcggc gcggcataaa gctggcgggt tgtcgctgcg ctacgaacgg
      721 ccggcgcccc gggccgccct acgctccaaa cggaacgccg cccgagctgc cctaggaatt
      781 tgaagcggcc gcagtaggcg agagtttttt cacaagctct ccgctctcag tcgcgataga
      841 acacctgcac caggtgatag ccgaactggc tcttcaccgg gccatgcact tcgcgcaggg
      901 gtttgttgaa gatgacctgg tcgatcgccc gcaccatctg tcccgggcgg actttgccaa
      961 gatcgccgcc acgcttgccg gaggggcagg tggaatactg gcgcgcgagc agatcgaagg
     1021 cctcgccggc ggcgatgcgc ttcttcaatg cggcggcttg cgcttcggtt ttcaccagaa
     1081 tatggcgggc cattgccttt gccatgatgg gctcctcatt tttaccgggt gcgtattgtg
     1141 ccagtattcg ttgcgccatc agaatgacgc cacttttaag actttccgat tcgccagggg
     1201 cagggttgaa tcctggccta aggtattaaa tccactcgat ggaagcgctg ctcatggata
     1261 tccactcaat gctgaaaatc ctggccagcc aggatggttc cgatctgtac ctgtccaccg
     1321 gtgcgccgcc gtgcgccaag ttcaatggcg tgctcaaggc gctcagccag gagcccttga
     1381 agcccggtga ggtggccgcc atcgccgacg cggtgatgga cgtcgcccag cgcgaggagt
     1441 tcgagcgcga actggagatg aacctggcga tttccctggc cggcgtcggg cgctttcgca
     1501 tcaacatctt caagcagcgc aacgaggtgt ccatagtcgc gcgcaacatc aagctggata
     1561 ttcccaagtt cgaagacttg aagctgcccg aggtgttgct cagcaccgtg atggagaaac
     1621 gcggcctggt gctgttcgtc ggcggcaccg gctcgggtaa atccacttcc ctggcggcgt
     1681 tgatcgacta ccgcaaccgc aatagcggcg gccacatcat caccatcgag gacccggtgg
     1741 agtatgtcca tcggcacaag aaatcgatca tcaaccagcg tgaagtgggg gtcgacaccc
     1801 gcagcttcca tgcggcgttg aagaacaccc tgcgccaggc accggacgtg atcctgatcg
     1861 gcgagatccg cgaccgcgag accatggagc acgccctggc cttcgccgac accggtcacc
     1921 tggcgatttc caccctgcac
//
LOCUS       DASWIY010000236        12683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_137879, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000236 DASWIY010000000
VERSION     DASWIY010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 12683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12683
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137879"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..2179)
                     /locus_tag="VGA59_08690"
     CDS             complement(131..2179)
                     /locus_tag="VGA59_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HEX9719795.1"
                     /translation="MQPTLSPARGLARWLWISGASIAAACFVAFAASPAQAQADPAPR
                     LKTESPYFFVKSDDPALDQLPLKSTRVDVRISGVIADVTVTQRYKNEGQRAIEAKYVF
                     PGSTKAAVYGMNVRLAERLITAQIREKQQARIEYDSAKKEGKTAALLEQHLPNVFQMN
                     VANIMPGDDVQVELRYTELLVPQSGNYQFAFPTVVGPRYNSPQSSQATAQWVSQPFLP
                     AGQEGGAAFDIQVAIASPIGIKEVRSASHDIEVTQQGDERAAISLQQGGAQNNRDFIL
                     DYRLAGERIESGVMLFRGSDENFFLAMVEPPKQLPASVISPREYIFVVDISGSMHGFP
                     LETAKVLLRELIGNLKPSDSFNVLLFSGSNRFLAPQSVPATRANIEQAINTINQMGGG
                     GSTELIPALKRVYAEPKAADVSRTVVVVTDGYVTVEREAFELVRRNLSQANVFSFGIG
                     SSVNRHLMEGLARAGMGEPFIITKPAQAAAEAARFRRMIESPVLTSVKARFEGLDVYD
                     VEPAQLPDVLGERPVVVFGKWRGSALGQVVIEGQSATGPYRNAVPVGAHIAQDSAALR
                     YLWARHRIAALSDQESLEGGDAQRKRITELGLHYNLLTQYTSFLAVDRVVRNLAPQDA
                     ASVNQPSPMPQGVSNLAIGAEVPGTPEPGTWGAMALLVSMLAMLARRARRNDPRRFTS
                     "
     gene            complement(2437..3873)
                     /gene="creC"
                     /locus_tag="VGA59_08695"
     CDS             complement(2437..3873)
                     /gene="creC"
                     /locus_tag="VGA59_08695"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HEX9719796.1"
                     /translation="MSKRNRIFIGILLIFAAGLAFMLYRVLADLDPRYRESAEESLVE
                     TSQLMASLVEQDVRDGAIDVTRLEPLFKSVYARQFEAQIFSLTKRQVELRLYVTDRSG
                     HVIFDSLGQVAGADFSRWRDVKLALRGEYGARTSPDLENDPATSVMYVGAPVRWKNDI
                     IGAVSVGKPVQSFGQFVEAARRKTLYVGLMSGAGVILLALIVSVWLVRPFGLIADYVR
                     YVRAQRRFSLPRLGRHAWGVIGAAYDEMRDALAGRNYVADYVQTLTHEVKSPLSAIRG
                     AAELLQEPRMPQGERQRFLANITRETQRIQEMIDRMMELTALETRRALEHVEDVALRP
                     LLEELAAEAQDAAGPRRIRIVLDAPEDAEVEGDPFLLRRAVSNLLDNAIDFSGDGGEV
                     RIALERTRRAARIRVQDQGPGIPEYAQDKVFEKFYSLARPHSNKKSTGLGLAFVREIA
                     SLHHGRIDLDNAHAGGAVATLSLPLGGA"
     gene            complement(3888..4613)
                     /gene="creB"
                     /locus_tag="VGA59_08700"
     CDS             complement(3888..4613)
                     /gene="creB"
                     /locus_tag="VGA59_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HEX9719797.1"
                     /translation="MLSKPRILVVEDESGIADTIQYVLATDGFQPVWCSTADAALREF
                     AAQPPALAILDVGLPDLNGFELFKQLQDLPGGRDIPMLFLTARSDEIDRVVGLELGAD
                     DYIAKPFSPRELVARVRTILRRSVRAPAAAPAKAAATPAQPFVLDEERRQIRYYGRAL
                     ELSRYEYGILRLLVQKPGRVFTRDELLDKVWGGDSDSFDRTVDAHIKTLRAKLKAVAP
                     NVEAIRTSRGAGYALSEELPPQP"
     gene            complement(4658..4822)
                     /locus_tag="VGA59_08705"
     CDS             complement(4658..4822)
                     /locus_tag="VGA59_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX9719798.1"
                     /translation="MLRITLGIVLIGLAATGNVGWWGWLGIVPLATGLIGWCPPYAML
                     GLSTCKLRKT"
     gene            complement(4880..5311)
                     /gene="cueR"
                     /locus_tag="VGA59_08710"
     CDS             complement(4880..5311)
                     /gene="cueR"
                     /locus_tag="VGA59_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HEX9719799.1"
                     /translation="MNAAALAWPVNIGMASRMSGVTPKMVRHYESLGLLPAVGRTDSG
                     YRQYTEAEVHTLRFIKRSRELGFPMDDIAELVGLWRNRRRASANVKRIAQKHSDELGL
                     RIEAMQAMQRTLQHLIHCCQGDERPDCPILDDLAGLPTAHA"
     gene            complement(5319..5516)
                     /locus_tag="VGA59_08715"
     CDS             complement(5319..5516)
                     /locus_tag="VGA59_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX9719800.1"
                     /translation="MNQVFDVQGMSCGHCERAVTQAVKAVDPTAEVKVDLGSGKVQVQ
                     TAQPREAIAKAIAEEGYPVAA"
     gene            5672..7876
                     /locus_tag="VGA59_08720"
     CDS             5672..7876
                     /locus_tag="VGA59_08720"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9719801.1"
                     /translation="MNTTLTAPTLDLGIGGMTCASCVARVERALKKVPGVQEANVNLA
                     TESARITYAPSEQMEARMRRAVRDAGYEPRAVEDTGAGDQSPWAGFAPVAAGLVLSAP
                     LLIPMIADLFGRHWMLPFWLQFLLATPVQFVLGARFYKAGWHAAKALTGNMDLLVAIG
                     TSAGWGLSVWLWLSAGSDHLYFEASAVVITLVLLGKWLEARAKQQTTAAIRALHALRP
                     EVAHVLSRAGEEVDLPVAEVLAGDRLVVRPGERMPADGVIEQGRTQVDESMLTGEPLP
                     VAKSEGAKLTGGSINGDGRVVMQVTAVGNETVLAHIIRLVEDAQAAKAPIQRLVDQVS
                     AVFVPVVLAIGLVTVLGWLAAGEPLETALIRAVAVLVIACPCALGLATPAAIMAGTGV
                     AAQHGVLIKDAQALELAHKVDTVAFDKTGTLTAGQPRLVGFVAVPGTDRAQALAVAAS
                     LQSGSEHPLARAVVAAAREQGLAIAQPDSVRSVAGRGTEGEIGVVSYLIGSLRWLEEL
                     GVPLGELQGEAQRLQRDGGTLSALVERTTQGLSLRALMAFADEPKAGAREALAALRAR
                     GLRLVMISGDNHAAALVMGERLGLHPEEVMAEVLPGEKAAKVAELKRHGHVVAMVGDG
                     VNDAPALAAADVGLAMGTGTDVAMHAAGITLMRGNVGLVGAALDISHRTVMKIRQNLF
                     WAFAYNVAGIPLAALGYLNPVLAGAAMALSSVSVMANALLLKRWREGSPGSR"
     gene            7936..8559
                     /locus_tag="VGA59_08725"
     CDS             7936..8559
                     /locus_tag="VGA59_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9719802.1"
                     /translation="MTHNALQIIRDEHAALAAVLRSLGMMLERGPLDEPERFFGVVRA
                     MLFYIDEFPERLHHPKESDLLFPKIARVAPELMPVIGRLESDHMQGEGRVRGLQHLLL
                     AWELIGESRRDVFMDAAREYLAFYLEHMRIEETQILPAAEKLLAPADWETLDEAFLAS
                     RDPLAGGLREPCYDRLFTRIVMTAPAPIGVGPTGIGLAGADAQASIG"
     gene            complement(8541..9644)
                     /locus_tag="VGA59_08730"
     CDS             complement(8541..9644)
                     /locus_tag="VGA59_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX9719803.1"
                     /translation="MPSLFDPVRAGTLHLPNRIVMAPLTRNRAPDAVPTPLMAEYYAQ
                     RASAGLLITEATAISAQGQGYADVPGLYGTDQLDGWKYVTEAVHARGGRIVVQLWHVG
                     RVSHVDLQPQGQKPVAPSAITARTKTYLIKDGVGSFVPTSEPRALDSEELPGIVHDYR
                     HAARHALSTAGFDGVEVHGANGYLLDQFLKTGANRRTDDYGGSIENRARLLLEVMRAV
                     AGEVGGGRTGLRISPVTPANDIHDENPQALFEYVVRQLAPLGLAWIHVIEGATGGARE
                     IADRPFDYDALRTAYRAAGGTGAWMVNNNYDKALAEQALANGADLVAFGKLFIANPDL
                     VERLRDNAPLNTPDKATFYGGGAEGYTDYPMLA"
     gene            9799..11070
                     /locus_tag="VGA59_08735"
     CDS             9799..11070
                     /locus_tag="VGA59_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719804.1"
                     /translation="MKRFMKWLAGIVLGLALLLAAVAVTLQYWVASDDFRARVSQQMS
                     GALGVPVQLGRVSVDLWPLPAVALEQVQLMSKPALTLEHIEARPVWAGLLRGRLVIAT
                     LVVRDAVVPEAAVAAIAAALQKPQRAPDNSAGESRGAIDFMPERTVLENVTWVHAKGG
                     STTLDAQAQLDPDGMPATAQLQVLEGRLEGAKATLQRQPDHWALRAAIGGGTVIGKLQ
                     LRRGAKGASLLQGQFDTANVEVSALTAPRRTLTGRLEAHTTWRAEFQRAGSLVDALQS
                     QTRFTVRNAVVHGVDLAQAVKSVGLNRGGETRLDTLAGNVSTQGRAVQLTNLAATSGV
                     LSANGNVAIAPNRSLSGRVTVDLASGAAGGAIGVPLAVGGTLDEPSVTLTRGALVGAA
                     VGTLLAPGVGTGAGAKLGDRLGEGLKGLFGK"
     gene            11168..12016
                     /locus_tag="VGA59_08740"
     CDS             11168..12016
                     /locus_tag="VGA59_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX9719805.1"
                     /translation="MHLSTVATVLEYEVKAPTHFCFNIEAAHWPTQKILSERLAVSSG
                     VAPHSFTDDVSGNRFFRFDASPGPLLVDYKADVEVYSEQVDEHLPQTPVGEVPDAIFK
                     YLMATRYCESDLLGSVAQQLFGNEEPGIARVRKVAKWIHDSIEYRPGSTNSTTTAQEV
                     LVQRAGVCRDFAHLGIALCRALNVPARLVVGYVWFDEPPQDFHAVFEAWFGGRWVLFD
                     PTGMAPVDRLVRIGTGRDAKDVAFSTFFGEVEMTRMELSVLEHEPRRTPDSGEAVVEL
                     AAAGSA"
     gene            complement(12198..>12683)
                     /locus_tag="VGA59_08745"
     CDS             complement(12198..>12683)
                     /locus_tag="VGA59_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX9719806.1"
                     /translation="FVIHSAVHEARSDVQCVLHTHTRAGVAVSAQKCGILPISQQSTF
                     VLASLAYHDYEGVAFREEEKPRLQADLGDANFLVLRNHGLLVTGQTIADAFLQMYTFE
                     NACRIQLDAQSGGDLVEVDPRIVSGVAEALRVQTGGLGGAFVWPSLIRKLDRLDPSYK
                     D"
BASE COUNT         1912 a         4211 c         4488 g         2072 t
ORIGIN      
        1 caagcctgcc ccggacttga tccggggtcc ggggcaggcc cgatccggga tctgtgaatt
       61 cggaaggcat ggattgcggg tcaaggcccg caatgacacg gcccgcaatg acagggcccg
      121 caatgacaac tcaagacgtg aagcgccggg ggtcgttgcg gcgcgcccgg cgcgccagca
      181 tcgccagcat cgagacgagc agcgccatcg cgccccaggt tcccggttcg ggcgtgcccg
      241 gcacttcggc gccgatggcc aggttgctca cgccttgcgg catcggcgaa ggctggttca
      301 cgctggccgc atcctgcggc gcgagattgc gcacgacgcg gtccaccgcc aggaagctgg
      361 tgtactgcgt cagcaggttg tagtgcaggc ccagctcggt gatgcgcttg cgctgcgcgt
      421 cgccgccctc cagcgactcc tggtcggaca aggcggcgat gcgatggcgt gcccacagat
      481 agcgcagcgc ggcgctgtcc tgggcgatgt gcgccccgac cggcaccgcg ttgcgatacg
      541 ggccggtggc gctctgccct tcgatcacga cctgcccgag ggcggagccg cgccacttgc
      601 cgaacaccac cacggggcgc tcgcccagca cgtccggcaa ttgggccggt tccacgtcgt
      661 acacgtccag gccctcgaag cgcgccttga cgctggtgag caccggcgac tcgatcatgc
      721 ggcgaaaacg cgccgcctcg gccgcggcct gcgccggctt ggtgatgatg aagggctcgc
      781 ccatgccggc gcgcgccagg ccttccatca ggtggcggtt cacggaagag ccgatgccga
      841 acgaaaacac attggcctgc gacaggttcc tgcgcaccag ctcgaatgcc tcgcgctcca
      901 cggtcacata gccgtcggtg accacgacga cggtgcgcga cacatcggcc gccttgggct
      961 cggcatagac ccgcttgagc gcgggaatca gctcggtgct gccgccgccg cccatctggt
     1021 tgatcgtgtt gatcgcctgc tcgatgttgg cccgggtggc cggcaccgat tgcggcgcca
     1081 ggaagcggtt gctgccggaa aacagcagca cgttgaaact gtcgctgggc ttcaggttgc
     1141 cgatcagctc gcgcagcagc accttggccg tctccagcgg aaagccgtgc atggagccgg
     1201 agatgtcgac cacgaagatg tactcgcgcg ggctgatcac gctcgccggc agctgcttgg
     1261 gcggctcgac catcgccaga aagaagttct cgtcgctgcc gcgaaacagc atcacaccgg
     1321 actcgatgcg ctcaccggcc agccggtagt cgaggatgaa gtcgcggttg ttctgcgcgc
     1381 cgccttgttg caggctgatg gcggcgcgct cgtcgccttg ctgcgtcacc tcgatgtcgt
     1441 gcgaggccga gcgcacttcc ttgatgccga tcgggctggc gatcgccacc tggatgtcga
     1501 aggccgcgcc accttcctgc ccggcgggca ggaaaggctg gctcacccat tgcgcagtcg
     1561 cctggctgga ctgcgggctg ttgtagcgcg gaccgaccac ggtgggaaag gcgaactggt
     1621 aattgccgga ctgcggcaca aggagttcgg tgtagcgcag ctccacctgg acatcgtcgc
     1681 cgggcatgat gttggccacg ttcatctgga acacgttggg caggtgctgc tccagcagcg
     1741 cggcggtctt gccttccttc ttcgcgctgt cgtattcgat acgggcctgc tgcttctcgc
     1801 ggatttgcgc ggtgatcagc cgttccgcca ggcgcacgtt catgccgtac accgccgcct
     1861 tggtggagcc cgggaacacg tacttggcct cgatcgcgcg ctgcccttcg ttcttgtagc
     1921 gctgggtcac cgtgacgtcg gcgatcacgc cggaaatacg cacatcgacg cgggtcgatt
     1981 tgagcggcag ctggtccagc gccgggtcgt cgcttttgac gaagaagtac gggctttcgg
     2041 ttttcaggcg cggcgcggga tcggcctggg cttgcgcggg gctggccgca aaggccacga
     2101 agcaggcggc cgcgatgctg gcgccgctga tccagagcca gcgtgccaag ccgcgggcgg
     2161 gtgagagtgt gggctgcatg ttcgctccaa gggcacagtg cgagggtgca ctggagcgaa
     2221 gcatcggcag cgcgcgcgaa gccggtttga agtgcagcgg gtgattctgt gaaatctgtg
     2281 tgaagtccgg aagactcatg gattgcggat cgggcctgcc ccggacttga tccggggtcc
     2341 gcaatgacaa gccttttttt gtcatgccgg acttgataag gtctgtcatt gcgggcttga
     2401 cccgcaatcc atgacttctg tgttgattcc cgtgtctcag gcgccgccca gcggcagcga
     2461 cagcgtggcc acggcgccgc ccgcgtgcgc attgtccagg tcgatgcggc cgtgatgcag
     2521 cgaggcgatc tcgcgcacga acgccaggcc caggcccgtg ctcttcttgt tggagtgcgg
     2581 acgcgccagc gaatagaact tctcgaacac cttgtcctgc gcgtactcgg ggattcccgg
     2641 gccctggtcc tgcacccgga tgcgcgccgc gcgccgggtg cgctccagcg cgatccgcac
     2701 ttcgccgccg tccccggaaa agtcgatcgc gttgtccagc aggttgctca cggcgcgccg
     2761 caacaggaag ggatccccct ccacctccgc gtcctcgggc gcatcgagga cgatgcggat
     2821 gcgccggggc ccggccgcgt cctgcgcttc cgccgccagt tcctcgagca ggggccgcag
     2881 cgcgacgtct tccacatgct cgagtgcgcg gcgcgtctcc agtgccgtga gttccatcat
     2941 ccggtcgatc atttcctgga tccgctgggt ctcgcgcgtg atgttcgcca ggaagcgctg
     3001 ccgctcgccc tgcggcatgc gcggctcctg cagcagttcg gcggcgccgc ggatcgccga
     3061 cagcgggctc ttgacctcgt gggtgagagt ctgcacatag tcggcgacgt agttgcggcc
     3121 ggccagcgca tcgcgcatct cgtcgtaggc cgcgccgatc acgccccagg cgtgccggcc
     3181 cagccgcggc aggctgaagc ggcgctgggc ccggacatag cgcacgtagt cggcgatcag
     3241 cccgaagggc cgcaccagcc agaccgacac aatcagggcc agcaggatca cgcccgcgcc
     3301 ggacatcagg ccgacataca aggtcttgcg gcgcgccgcc tcgacgaact ggccgaagct
     3361 ttgcaccggc ttgcccaccg acacggcgcc gatgatgtcg ttcttccagc gcaccggcgc
     3421 gcccacgtac atcaccgatg tcgccgggtc gttttcgaga tcgggcgagg tgcgcgcgcc
     3481 gtattccccg cgcagcgcca gcttgacgtc gcgccagcgc gagaagtcgg ccccggccac
     3541 ctggcccagg gaatcgaaaa tcacgtgccc gctgcggtcc gtcacgtaca gccgcaactc
     3601 gacctggcgc ttggtcaggc tgaagatctg ggcctcgaac tggcgggcgt agaccgactt
     3661 gaacagcggc tccaggcgcg tgacgtcgat ggcgccgtcg cgcacgtcct gctcgaccag
     3721 gctggccatc agctgcgagg tttcgaccag cgattcctcg gccgattcgc ggtagcgcgg
     3781 gtcgaggtcg gcgagcacgc gatacagcat gaaggccagc cccgccgcga agatcagcag
     3841 gatgccgatg aagatgcggt tgcgtttgct caagccgttc gctttggcta aggttgcggc
     3901 ggaagctctt cgctcagcgc gtagccggcg ccgcgcgaag tgcggatcgc ctccacgttg
     3961 ggcgcgacgg ccttgagctt ggcgcgcagc gtcttgatgt gggcgtccac cgtgcgatcg
     4021 aagctgtcgc tgtcgccgcc ccagaccttg tccagcagtt cgtcgcgcgt gaacacccgg
     4081 ccgggctttt gcaccagcag cctcaagatg ccgtactcgt aacgtgaaag ttcgagcgcg
     4141 cggccgtaat agcggatctg gcggcgctcc tcatccagga cgaagggctg cgcgggggtg
     4201 gccgctgcct ttgccggcgc ggccgcggga gcgcgcacgc tgcgccgcag gatggtgcgc
     4261 acgcgcgcca ccagttcgcg cggcgaaaag ggcttggcga tgtaatcgtc ggcgcccagc
     4321 tccagcccca ccacgcggtc gatctcgtcg ctgcgcgccg tgaggaacag catcgggatg
     4381 tcgcgcccgc cgggcaggtc ctgcagttgc ttgaacagct cgaaaccatt gaggtccggc
     4441 aagccgacat cgaggatggc cagcgccggt ggttgcgcgg cgaattcacg cagcgccgca
     4501 tcggccgtgc tgcaccacac aggctggaag ccgtcggtgg ccagcacgta ctggatcgtg
     4561 tcggcgatgc cggactcgtc ttccaccacc aggatgcggg gcttgctgag catggcccct
     4621 atggtagcgg cgcgcggtcc gctgtccgcg ccgggggtca ggtcttgcgc agtttgcagg
     4681 tgctcaagcc cagcatggca tagggcgggc accagccgat gaggccggtg gcgagcggca
     4741 cgatgcccag ccagccccac cagccgacgt tgccggtggc ggccaggccg atcagcacga
     4801 tgccgagagt gatgcgcagg atgcggtcga cggcaggccg ggtgacacgc ccgccagcgc
     4861 cggatacagc gccagcggat caggcatgag ccgtgggcaa cccggccaga tcgtccagga
     4921 tcgggcagtc gggacgctcg tcgccctggc agcagtgaat cagatgctgc agggtccgct
     4981 gcatggcctg catggcttcg atgcgcaggc ccagctcgtc ggaatgcttc tgcgcaatgc
     5041 gcttgacgtt ggcgctggca cgccggcggt tgcgccacag gccaaccagc tcggcaatgt
     5101 cgtccatcgg aaagcccagc tcgcgcgagc gcttgatgaa ccgcagcgtg tggacctcgg
     5161 cctcggtgta ctggcggtag ccgctgtcgg tgcggcccac ggccggcagc aggccgagcg
     5221 attcgtagtg gcgcaccatc ttgggcgtca cccccgacat gcgcgacgcc attccgatgt
     5281 tgacaggcca ggccaatgca gctgcgttca tgggaacctc atgcggcgac gggataacct
     5341 tcttcggcga tggccttggc gatcgcctcg cgcggctggg cggtctgcac ctgcaccttg
     5401 ccgctgccca ggtcgacctt gacctcggcc gtcgggtcga ccgccttcac ggcctgggtg
     5461 accgcccgct cgcagtggcc gcagctcatg ccctgcacgt cgaatacctg gttcatgatg
     5521 ggatcctttc agaatcggtt gaaaagaagc atagcctgaa ccttgtcacc gtgggaaggt
     5581 caacccctat gccgcctgcc ttttcggcgc cggggttgac gcaaatcaac ccttgacctt
     5641 gccatggtgg caaggtgcat ccttgccccc aatgaatacc accctcaccg cacccacact
     5701 ggaccttggc atcggcggca tgacctgcgc gtcctgcgtc gcccgcgtcg aaagggcgct
     5761 caagaaggtg cccggcgtgc aggaagccaa cgtcaacctg gcgaccgagt cggcccgcat
     5821 cacttacgcg ccgtccgagc agatggaggc ccggatgcgc cgcgcggtgc gcgacgcggg
     5881 ctacgagccg cgtgccgtcg aggacaccgg ggccggcgac cagtcgccct gggccggatt
     5941 tgcgcccgtg gccgccggcc tggtgctgtc ggcgccgctg ctcattccca tgatcgcgga
     6001 cctgttcggg cgccactgga tgctgccgtt ctggctgcag ttcttgctgg ccacgccggt
     6061 tcagttcgtc ctgggcgcgc gtttttacaa ggccggctgg catgcggcga aagcgctgac
     6121 cggcaacatg gacctgctgg tggccatcgg caccagtgcc ggctggggcc tgtcggtctg
     6181 gctgtggctg agcgcgggaa gcgatcacct gtacttcgag gcttccgctg tcgtgatcac
     6241 cctggtgctg ctgggcaagt ggctggaggc gcgggccaag cagcagacca cggcggcgat
     6301 ccgggcgctg catgcgctgc ggccggaagt cgcgcatgtg ctgtcgcgcg ccggcgagga
     6361 agtggacctg ccggtggccg aggtgctggc gggcgaccgg ctggtggtgc ggccgggcga
     6421 gcgcatgccc gccgacggtg tgatcgagca gggccgcacc caggtggacg aatcgatgct
     6481 caccggagag ccgctgccgg tggcgaagtc ggagggcgcg aagctcaccg gcggcagcat
     6541 caacggcgac ggccgcgtgg tcatgcaggt cacggcggtc ggcaacgaga ccgtgctggc
     6601 gcacatcatt cgcctggtgg aggacgcgca ggcggccaag gcgccgatcc agcggctggt
     6661 ggaccaggtt tcggcggtgt tcgtgccggt ggtgctggcg atcggcctcg tcacggtgct
     6721 cggctggctc gcggccggcg agccgctgga aacggcgctg atccgcgcgg tggcggtgct
     6781 ggtgatcgcc tgcccctgcg cgctcgggct ggcgacgccg gcggcgatca tggccggcac
     6841 cggggtggcc gcccagcatg gcgtgctgat caaggatgcg caggcgctgg aactggccca
     6901 caaggtggat acggtggcct tcgacaagac cggcacgctg accgcgggcc agccccggct
     6961 ggtgggcttc gtcgcggtgc ccggtaccga tagggcgcag gcgctcgccg tggcggccag
     7021 cctgcaaagc ggcagcgagc atcctctggc ccgcgccgtc gtggcggcag ccagggagca
     7081 gggcctggcg attgcgcagc ccgacagcgt gcgttccgtc gccgggcgcg gcaccgaagg
     7141 cgaaatcggc gtcgtcagtt acctgatcgg cagcctgcgc tggctggagg aactcggcgt
     7201 gccgctgggc gaactgcaag gcgaggcgca gcggctgcag cgggacggcg gcaccttgtc
     7261 ggcgctggtc gaacgcacca cgcagggcct gtcgctgcgc gccctgatgg cgttcgccga
     7321 cgagcccaag gcgggcgcgc gcgaggcgct ggccgcgcta cgggcccgcg ggctgcggct
     7381 ggtgatgatc tcgggcgaca accatgcggc ggccctggtc atgggcgagc ggctcggttt
     7441 gcaccccgag gaagtgatgg ccgaagtcct gcccggcgag aaggcggcga aggtggccga
     7501 gctcaagcgc cacgggcatg tcgtggcgat ggtgggcgat ggcgtgaacg acgcgccggc
     7561 gctggccgcg gccgacgtgg gcctggcgat gggcaccggc accgacgtgg ccatgcacgc
     7621 cgcgggcatc accttgatgc gcggcaacgt ggggctggtg ggcgccgcgc tggacatctc
     7681 gcatcgcacc gtgatgaaaa tccggcagaa cctgttctgg gccttcgctt acaacgtcgc
     7741 cggcatccct ttggcggcgc tgggctatct gaacccggtg ctggcgggcg cggcgatggc
     7801 gctgtccagc gtcagcgtca tggccaacgc gctgcttctc aagcgctggc gcgagggcag
     7861 ccccggatct cgttgacctg gatcaacggg aggcccggtt ggccatgcca agctgccgcc
     7921 cacggagccc ctttcatgac gcataacgcc ctccaaatca tccgcgacga acacgccgcg
     7981 ctcgccgccg tgctgcgttc gctcggcatg atgctcgaac gcggcccgct ggacgagccc
     8041 gagcgctttt tcggcgtcgt gcgcgcgatg ctgttctaca tcgacgagtt ccccgagcgg
     8101 ctgcaccatc ccaaggaatc cgacctgctg tttcccaaga tcgcgcgcgt cgccccggag
     8161 ctgatgccgg tgatcgggcg gctcgagagc gaccacatgc agggcgaggg cagggtgcgc
     8221 ggcttgcagc acctgctgct ggcatgggag ctgatcggcg agtcgcggcg tgacgtcttc
     8281 atggacgcgg cgcgggagta tctggcgttc tacctggaac acatgcggat cgaggaaacg
     8341 cagatcttgc cggccgcgga aaagctgttg gcgccggccg actgggagac gctggacgaa
     8401 gccttcctgg cctcgcgcga tcccctggcc ggcggcctgc gcgagccctg ctatgaccgc
     8461 ctgttcaccc gcatcgtgat gacggcaccg gcgccgatcg gagtcggtcc gaccggcatc
     8521 ggcctggccg gcgccgacgc tcaggccagc atcgggtaat cggtgtagcc ctcggcgccc
     8581 ccgccgtaga aagtggcctt gtcgggcgta ttcagcggcg cgttgtcgcg cagccgctcc
     8641 accaggtcgg ggttggcgat gaagagcttg ccgaaggcga ccaggtcggc gccgttggcc
     8701 agggcctgct cggccagcgc cttgtcgtag ttgttgttca ccatccaggc gccggtgccg
     8761 cccgccgcgc ggtaagcggt gcgcagggcg tcgtaatcga acggccggtc cgcgatttcg
     8821 cgcgcgccgc cggtggcgcc ttcgatcacg tggatccagg ccaggccgag cggcgccagt
     8881 tgccgcacca cgtactcgaa caaggcctgc ggattctcgt cgtggatgtc gttggcgggg
     8941 gtgaccggcg agatgcgcag gcccgtgcgg ccgccgccga cctcgccggc cacggcgcgc
     9001 atgacttcca gcagcagccg ggcgcggttc tcgatcgagc ccccatagtc gtcggtgcgc
     9061 cggttggcgc cggtcttgag gaactggtcc agcaggtagc cgttggcgcc atgcacctcg
     9121 acaccgtcga agccggcggt gctgagcgcg tgccgggccg cgtggcgata gtcgtgcacg
     9181 atgcccggaa gttcctcgga atcgagtgcg cggggctccg acgtcgggac gaaggagccc
     9241 acgccgtcct tgatgaggta ggttttggtc cgggccgtga tggccgaggg cgcgaccggt
     9301 ttctggccct ggggctgcag gtccacgtgc gagacgcggc cgacatgcca cagctgtacg
     9361 acgatccggc cccctctggc gtgcaccgcc tcggtcacgt acttccatcc gtcgagctgg
     9421 tcggttccat acagcccggg cacatcggca tagccctgcc cctgcgcgct gatcgccgtg
     9481 gcctcggtga tcagcaggcc ggcgctggca cgctgggcgt agtactcggc catcaggggc
     9541 gtcggcacgg cgtcgggcgc gcggttgcgc gtcagcggcg ccatgacgat gcggttgggc
     9601 aggtgcagcg ttcccgcccg cacagggtcg aacaaacttg gcatggcgga tctcctggtt
     9661 gatggggccg gggccgcctt cgtcgccccg gcggccttcg gggggctttt tcgcggctgt
     9721 gtgacgatgc gtccatggct gtgtgacgat gcgtacatgc taatcgctgg acgctgtttc
     9781 tgcagcgaca atgcggccat gaagcggttc atgaaatggc tggcggggat cgtgctgggg
     9841 ctggcgctgc tgctggcggc ggtggccgtc accctgcagt actgggtcgc gagcgacgac
     9901 tttcgcgccc gcgtgagcca gcagatgtcc ggcgccctgg gcgtgccggt gcagttgggc
     9961 cgcgtgtcgg tggacctgtg gccgctgccg gcggtggcgc tggaacaggt gcagctcatg
    10021 agcaagcccg cgctgacgct cgaacacatc gaagcccgcc cggtctgggc cggtttgctg
    10081 cgggggcgcc tggtgatcgc caccctggtc gtgcgcgacg cggtggtgcc ggaggcggcg
    10141 gtcgccgcga tcgccgccgc cctccagaag ccgcagcgcg caccggacaa tagtgccggc
    10201 gagtcgcgtg gcgcgatcga cttcatgcct gagcgtactg tcctggaaaa cgtcacgtgg
    10261 gtgcacgcca agggcggcag caccaccctc gatgcgcagg cccagctcga tcccgacggc
    10321 atgccggcga cggcgcagtt gcaggtgctc gagggccgac tcgaaggcgc caaggcgacg
    10381 ctgcagcggc aacccgatca ctgggcgctg cgcgcggcga tagggggcgg caccgtgatc
    10441 ggcaagctcc agctgcggcg cggtgccaag ggggcttcgc tgctgcaagg ccaattcgac
    10501 acggccaacg tcgaggtttc cgccctgacg gcgccgcgcc gcacgctcac cggccggctc
    10561 gaagcgcaca ccacctggcg cgcggagttc cagcgggccg gctcgctcgt cgacgcgctg
    10621 caaagccaga cgcggttcac ggttcgcaac gccgtggtgc acggcgttga tctggcgcag
    10681 gccgtgaaat ccgtcggcct gaatcgcggc ggcgagaccc ggctggacac gctggccggc
    10741 aacgtgtcga cccaagggcg tgccgtgcag ctcacaaacc tcgccgccac ctcaggcgtg
    10801 ctgtcggcca acggcaatgt ggcgatcgcg cccaatcgca gcctgagcgg ccgcgtcacg
    10861 gtggacctgg catcgggggc ggccggcggc gccatcggtg tgccgctggc ggtgggcggc
    10921 acgctcgacg agccatcggt gacgctcacg cgcggcgcgc tggtgggcgc cgcggtcgga
    10981 accttgctcg ccccgggcgt gggcaccggt gccggcgcca agctcgggga ccggctgggg
    11041 gaaggcctga agggactgtt cggcaagtag cgcgccgtcc tacagcgcgc cgcgatggct
    11101 gctgatcgat tggcacagcg gtaccgctac catgggtcga accacgcccc tgcacaggta
    11161 accgagcatg catctttcca ccgtcgcgac cgttctcgaa tacgaggtca aggcgccgac
    11221 gcatttttgc ttcaacatcg aggcggccca ttggccaacg cagaagatcc tcagcgagcg
    11281 gctcgccgtc tcttccgggg tggcgccgca cagttttacg gacgacgtga gcggcaaccg
    11341 cttcttccgc ttcgatgctt cacccgggcc gctgctggtg gactacaagg cggatgtcga
    11401 ggtgtattcg gaacaggtcg acgagcacct gccgcaaacg ccggtcggcg aagtgcccga
    11461 cgcgatcttc aagtacctga tggccacccg ctactgcgaa tccgacttgc tcggcagcgt
    11521 ggcgcagcag ctgttcggca acgaggagcc gggcatcgcg cgggtgcgca aggtcgccaa
    11581 atggatccac gactcgatcg aataccggcc gggcagcacc aattccacca ctaccgcgca
    11641 ggaagtattg gtgcagcgcg ccggcgtgtg ccgcgacttc gcgcacctgg gcatcgcgct
    11701 gtgccgcgcg ctcaatgttc cggcacgcct cgtggtcggc tacgtctggt tcgacgagcc
    11761 gccgcaggat ttccacgcgg tcttcgaggc ctggttcggc gggcgctggg tgctgttcga
    11821 cccgacgggc atggcgccgg tcgaccggct ggtgcgcatc ggcaccggcc gcgatgccaa
    11881 ggacgtcgcc ttcagcacct tcttcggcga ggtcgagatg acacggatgg aactgagtgt
    11941 gctcgagcac gagccgcgca ggacgcccga cagcggcgag gcggtggtgg agctcgcggc
    12001 tgccggatcg gcgtgaatgg cagtaagggt gtcatgccgg gctgggctcg cggaacgccc
    12061 ggggcgcgca gctgtccagt gcagcgagcg caatgccggg cctacgctat gcacgtgccg
    12121 aggcatcacg ctccggcccg gccccggcat ccatgacttt tagcgggccg agcccgcact
    12181 gcggcgcaca cggacactca gtccttgtag ctgggatcga ggcggtccag ttttcggatc
    12241 agggaaggcc agacgaacgc accgccgagg ccgccggtct gcacccgcaa ggcctcggcc
    12301 accccgctga cgatccgcgg gtccacctcg accagatcgc cgccggattg cgcgtcgagc
    12361 tggatgcggc aagcgttctc gaatgtgtac atctgcagga acgcatcggc aatggtctgg
    12421 ccggtgacca gcaggccatg gttgcgcagc accaggaagt tcgcgtcacc caggtccgcc
    12481 tgcagccgcg gcttctcctc ttcgcggaag gccacgcctt cgtaatcgtg gtaggccagc
    12541 gacgccagca cgaaagtcga ttgctgcgag atcggcagga tgccgcactt ctgcgcgctc
    12601 accgccacgc cggcgcgcgt gtgcgtgtgc agcacgcact gcacgtcgct tcgtgcctcg
    12661 tgcactgcgc tgtggatcac gaa
//
LOCUS       DASWIY010000237        13333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1637568, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000237 DASWIY010000000
VERSION     DASWIY010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13333
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637568"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..1927)
                     /gene="nadB"
                     /locus_tag="VGA59_08750"
     CDS             complement(323..1927)
                     /gene="nadB"
                     /locus_tag="VGA59_08750"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX9719807.1"
                     /translation="MNPDSRDFDVLIVGSGLAGLSAALHLAPNHRVAVLTKRAMSDGS
                     SGWAQGGIAAVMGEDDSFASHVDDTLVAGAGLSDLAATRFVVEHAPESIAWLRQMGVP
                     FSQEAGHLHLTREGGHSARRIVHVTDATGAAVQQTLIDRVHRTPNITVFERHTLVDLI
                     TSRKIGLPGSAQPGVAGRCLGLYALDEATDEVFTFRAPQTVLATGGAGKVYLYTTNPD
                     TATGDGIAAAWRAGCRVTNMEFIQFHPTCLYHPNVKSFLISEAVRGEGGRLLLPVSAG
                     GTRFMPQHDPRAELAPRDVVARAIDFEMKKHGVDCVYLDISHQPAAFVREHFPNIYAR
                     CLDLGIDITRQPIPVVPAAHYTCGGVHTDLSGRTDLEGLHAIGETAYTGLHGANRLAS
                     NSLVECMVFARAAAQNIRSAPLPAPPALPAWDESRVTDADEAVVISHNWDELRRFMWD
                     YVGIVRTNKRLERAAHRIRLLQEEIQEFYRNFHVTRDLLELRNLVTVADLIVRSAQGR
                     HESRGLHFSRDYPEMDPDSKPTTLVP"
     gene            complement(2063..2920)
                     /locus_tag="VGA59_08755"
     CDS             complement(2063..2920)
                     /locus_tag="VGA59_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX9719808.1"
                     /translation="MTHAGEATLPEPPATELLPSMPDVVDQVALARLYGEPLFAMPTD
                     LYIPPDALEVFLEAFEGPLDLLLYLIRRQNFNILDIPMAGVTRQYLVYVDEIRGRNLE
                     LAAEYLLMAAMLIEIKSRMLLPPRKAAEGQEPEDPRAELVRRLLEYEQMKLAAARLNE
                     VPQMGRDVLRSQVYIEQALQPRFPDVNVVDLQEAWRDIVKRAKLVQHHKITREELSVR
                     EHMSIVLRRLQGRRFVEFEHLFDPARGVQVLIVTFIALLELAKEALIDVTQAEAFAPI
                     YVRLAYQPS"
     gene            complement(2917..3135)
                     /locus_tag="VGA59_08760"
     CDS             complement(2917..3135)
                     /locus_tag="VGA59_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEX9719809.1"
                     /translation="MSILQRIFRRPDYNSDATLLIEQLKTQKPDLDARQRAGMAIFWD
                     KHVDREAWRDWRDAQVPQKPYVYQTESK"
     gene            3390..5405
                     /gene="hyfB"
                     /locus_tag="VGA59_08765"
     CDS             3390..5405
                     /gene="hyfB"
                     /locus_tag="VGA59_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEX9719810.1"
                     /translation="MDLTGWLRLDWILVAVVGGWLLIGVAGVLGLRRFTFVSRVLFPA
                     GGALGLLLFGLALGAIFGAPEVAVLPIGLPNLPFHLRLDSLSAFFLMVIGAVSAGIST
                     FAAGYFRKAEGTPPGLLCLEYHVFLASMALVVLADDAYAFMVMWETMALSSYFLVTAN
                     HRVPEVRRAGYLYLTMAHIGAISILLCFGVLQANTGDYTFDNMRAQNLSPFWASVGFL
                     LALLGFGAKAGLVPLHVWLPEAHPAAPSPVSALMSGVMLKTAIYGLLRVSFDLLQTRF
                     WWWGGLLLALGLATALFGVVFAAVQVDMKRLLAYSSVENIGLVIASIGLALIFSGYGM
                     KALAALALTAALYHVASHAFFKSLLFLCTGCVLHATAERSLGKLGGLIRYMPWVAWVT
                     LIGVLTSAGLPPLGGFVSEWLLLQSFLFTPGLPDALLNMLIPAAAALVALVAALAGYT
                     MVKFFGVIFLGRPREEKLAQAHDAGAWERVGMLWLVLGCVALGLAPVQFIQIIDPVTQ
                     QLLAAGLGRTVAASGWLLAPISVERASYGPVIFLIGVAASFALAFLMVRWFYHGRLRR
                     SPAWDCGFPWQNARMQDTAEGFGQPIRQIFEPFFVMRRELPTPFDARPRYRVVVEDHF
                     WGWIYLPLAALAERIARLVGLLQQGRISIYLLYSFVTLIAMLLVVKR"
     gene            5402..6349
                     /locus_tag="VGA59_08770"
     CDS             5402..6349
                     /locus_tag="VGA59_08770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX9719811.1"
                     /translation="MNIYGVISQALEIVVALALAPLLTGWVNQWRAWLQNKSAPSLWQ
                     PYRVLHKLFNKDSVVADHASPLFRAVPYIVFGCMALASSIVPTLSTDLPLAPAADAIA
                     LVGLFALARVFISLAAMDIGTSFGSLGARREMLIGFLAEPALLMVLFSASLISKSTSL
                     TTMVEALAHQELAIYPALAFAGVAFTMVSLAENARVPVDNPATHLELTMIHEALILEY
                     SGRHLALIEWAASLKLFAYSCIGLALFFPWGVAEADAPLALLLALPVLVGKLAIGGFL
                     LAVLETLAAKMRIFRVPEFLAGAFLLAVIGMLVHILLAT"
     gene            6346..7005
                     /locus_tag="VGA59_08775"
     CDS             6346..7005
                     /locus_tag="VGA59_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HEX9719812.1"
                     /translation="MSELLAQFVNLLGALLLMLAFAMISQRRILTLINLFTLQGLVLV
                     VSTAVVGYVTGQPHLYLSAGLTFVLKVVLIPVLLHRIIDRLEIRWDVETLINIPTTML
                     IGIGVVIFAFNLATPIAMLSTSLARGTLGIALACVLLSFLMMITRAKAVPQVIGFLAM
                     ENGLFFAATSATYGMPMVVELGIALDVLIGVLILGVFMFQIREQFDSLDIRHLEKLKD
                     Q"
     gene            7172..8626
                     /locus_tag="VGA59_08780"
     CDS             7172..8626
                     /locus_tag="VGA59_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HEX9719813.1"
                     /translation="METLFVVLGIPLAGGITLALLGHRDAARDINVAFSLGTFIAACF
                     LTARIVADGPMFVWNEEFFIDPLNVFLVTLTAFVGLTTAIFSRPYMRVERDHGKMTPN
                     RMRLYHSMYQLFSFTMLLALMTNNMGILWVAMEAATLTTVLLVSVYRTAASLEAAWKY
                     FILCGVGIAQALFGTVLLYMAAEKVLGSEGGALLWTHLDAVKDRLDPNIMTLAFAFLF
                     IGYGTKVGLVPLHNWLPDAHAEGPTPVSAVLSGLLLNVALYAVLRCKVLTDGALQSHL
                     AGNLMMGFGLVSVVASVFLLIRQKDVKRMFAYSSIEHMGLMTFAFGMGGPIANFAGLL
                     HMTVHSLVKSAIFFAVGHAAQKAGTQMMDAIRGLIKVSPTVGWGLMLGSLAILGMPPF
                     GVFASEFLIVTTAMREQPWATPFLLVALGVAFASVFSRVQPMVFGDTSVKPLAHPPAL
                     IPVFVHLGLALMLGLYIPPYLDMWYRQAARMLGN"
     gene            8628..10187
                     /locus_tag="VGA59_08785"
     CDS             8628..10187
                     /locus_tag="VGA59_08785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9719814.1"
                     /translation="MATIEALNFQLERLPAPLPLWRAGVDAFQWVAAARAMAQAGGRL
                     VSLWGCDRSLGPATALAVSAAYAVKEGMVWLDLALGERDTAYPDLAGIFPFAARMQRA
                     TADLLGIAAQGSEDGRPWLNHGAWPEGYFPLRRDATSGVGSMSPQPVRDYPFVPVEGD
                     GVHEIPVGPVHAGIIEPGHFRFSVVGEKVLRLEQRLGYTHKGIEQRFTRLAPLEAHKL
                     AGRVSGDSTVAFAWAYCMALESAAGCDVPERAQWLRALLLERERVANHLGDLGALGND
                     AALAFGLAQFSRLREDWLRLSGEAFGHRLMMDRVVPGGVAGDVDAAMRRRLQHQCDAI
                     EAEVRALEAIYESHAGLQDRFATTGIVAPQLAAQLGLAGLAGRASGQPADLRCDQSWA
                     PYERLRVKAAVRQNGDVAARVAVRFEETLESLRLIREICHSLPEGPLRGAVELPRKAA
                     TGAGWIEGWRGEVFVALELGADAGIRRCHCHDPSWQNWPVLEHAVIGNIVPDFPLINK
                     SFNLSYSGHDL"
     gene            10225..10740
                     /locus_tag="VGA59_08790"
     CDS             10225..10740
                     /locus_tag="VGA59_08790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX9719815.1"
                     /translation="MWKIVRQIARAGIRTEAPPFATGEDSPVAVERIQKEILDILGQA
                     LAIRQVDAGSCNGCELEIHALNNPYYNLEGLGIRFVASPRHADMLLVTGPVSRNMEMA
                     LRRTYEATPEPRLVVAVGDCGCTGGIFGESYATCGAVANVLPVDATVPGCPPPPIEIM
                     RGILSAVRRRR"
     gene            complement(10817..12647)
                     /locus_tag="VGA59_08795"
                     /pseudo
     CDS             complement(10817..12647)
                     /locus_tag="VGA59_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611581.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
     gene            complement(13029..13124)
                     /locus_tag="VGA59_08800"
                     /pseudo
     CDS             complement(13029..13124)
                     /locus_tag="VGA59_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
BASE COUNT         1988 a         4376 c         4441 g         2528 t
ORIGIN      
        1 gccgaacatc ggcgcggcct gaaatgttgt catccggacc ccggatcaag tccggggcag
       61 gcctgatccg gggacccggg gccatggatt gcggatcaag tccgcaatga caaccaagag
      121 tcaccttgtc atgccggacc ccggatcaag tccggggcag gcctgatccg gcatccatgc
      181 attcggctga gatggattgc ggcgccgggc cgcagcgtga tgcctcggca cgtgcatagc
      241 gcaggcccgg cattgggctc gctacactgg acagctgcgc gccccgggcg taccgcgagc
      301 ccagcccgca atgacaatcc cttcagggaa ccagggtcgt cggcttcgag tccgggtcca
      361 tctcgggata gtcgcggctg aaatgcaggc cgcggctctc gtggcgcccc tgcgccgagc
      421 ggacgatcag gtcggccacc gtgaccaggt tgcgcaactc gagcaggtcg cgcgtgacgt
      481 ggaagttcct gtagaactcc tggatctctt cctgtagcag gcggatgcgg tgggcggccc
      541 gctccagccg cttgttggtc cgcacgatcc ccacgtagtc ccacatgaag cggcgcagct
      601 cgtcccagtt gtgcgagatc accacggcct cgtcggcatc ggttacccgg ctttcgtccc
      661 acgccggcag ggcgggaggc gcgggcaggg gggcggaccg gatgttctgg gcggcggcgc
      721 gggcgaacac catgcactcc accagcgaat tgctggccag gcggttggcg ccatgcagcc
      781 ccgtgtacgc ggtttcgccg atcgcgtgca gtccttccag gtcggtgcgg cccgagaggt
      841 ccgtgtggac gccgccgcag gtgtagtgcg cggccggcac caccgggatc ggctggcggg
      901 tgatgtcgat gcccaggtcc aggcatcgag catagatgtt ggggaagtgc tcgcggacga
      961 acgccgccgg ctggtgtgaa atgtccaggt acacgcagtc cacgccgtgc ttcttcatct
     1021 cgaagtcgat ggcccgggcc accacgtcgc gcggcgccag ttcggcgcgc gggtcgtgct
     1081 gcggcatgaa gcgcgttccg ccggccgaga ccggcagcag caggcgtcca ccctcgccgc
     1141 ggaccgcttc cgagatcagg aaggacttga cgttggggtg atacaggcag gtcggatgga
     1201 actggatgaa ctccatgttc gtcacccggc agcccgcgcg ccaggccgcc gcgatgccgt
     1261 cgccggtcgc ggtgtcgggg ttggtggtgt agagatagac cttgccggcc ccacccgtgg
     1321 ccaggacggt ctgcggggcc cggaaggtga agacctcgtc cgtggcctcg tcgagcgcgt
     1381 acaggcccag gcagcgcccg gccacgccgg gttgtgcgct gccgggcagg ccgatcttgc
     1441 ggctggtgat caggtccacc agcgtgtgcc gctcgaacac ggtgatgttg ggggtgcggt
     1501 gcacccggtc gatcagggtc tgctgcacgg cggcgcccgt ggcatcggtc acgtgcacga
     1561 tgcgccgggc gctgtggccg ccttcgcgcg tcaggtgcag gtggccggct tcctgcgaga
     1621 acggcacccc catctggcgc agccaggcga tgctttccgg cgcgtgttcg accacgaaac
     1681 gggtggccgc caggtccgac aggccggcgc cggcgaccag cgtgtcgtcc acatgggacg
     1741 cgaagctgtc gtcctcgccc attacggcgg caatgccgcc ctgggcccag ccgctggagc
     1801 cgtcgctcat cgctcgcttg gtcagcaccg cgacgcgatg attaggtgcc aggtgcagcg
     1861 cggccgacag gccggccagg ccgctgccga cgatcagcac gtcgaagtcg cgggaatcag
     1921 gattcatgat gttctaggtc ctgttcacac taatggcaag tgcgaacgtg gttaaaacag
     1981 cgccaatcta ggcgcgcgac gacgcccagg ctggacgcct gggctaggag cgtaacaacg
     2041 agtggcgctg ttttaaccac gttcaggacg gctggtaggc cagccggacg tagatcgggg
     2101 cgaaagcctc ggcctgggtc acgtcgatga gagcctcttt cgccagttcc agcaacgcga
     2161 tgaaggtgac gatcagcacc tgcacgccgc gcgccggatc gaacagatgc tcgaactcga
     2221 cgaacctgcg gccctggagg cggcgcagca cgatgctcat gtgctcgcgc acggaaagct
     2281 cctcgcgggt gatcttgtgg tgctgtacca gcttggcccg tttgacgatg tcgcgccagg
     2341 cttcctgcag gtcgaccaca ttgacgtcgg gaaaccgcgg ctgcagcgcc tgctcgatat
     2401 agacctggga gcgcaggacg tcgcggccca tctggggcac ctcgttcagg cgcgcggccg
     2461 cgagcttcat ctgctcgtat tcgagcaggc ggcgcaccag ctcggcgcgc gggtcctcgg
     2521 gttcctgtcc ctccgcggcc ttgcgcgggg gcagcagcat gcgcgacttg atctcgatca
     2581 gcatcgcggc catcagcagg tattccgccg ccagctccag gttgcggccg cggatttcgt
     2641 ccacgtacac caggtactgg cgcgtgaccc cggccatcgg aatgtcgagg atgttgaagt
     2701 tctgcctgcg aatcaggtaa agcagcaggt cgagcggccc ttcgaacgcc tcgagaaaga
     2761 cctccagcgc gtccggcgga atgtacaagt cggtcggcat ggcgaacagc ggttcgccgt
     2821 acagccgtgc cagcgccacc tggtccacca cgtcgggcat gctgggcagc agctcggttg
     2881 ccgggggctc cggcagcgtc gcttcgcccg catgagtcat ttcgattcgg tctggtaaac
     2941 gtacggtttt tgcggcacct gcgcgtcgcg ccagtcgcgc caggcctcac ggtccacgtg
     3001 cttgtcccag aagatcgcca tgccggctcg ctggcgcgcg tccaggtccg gcttttgcgt
     3061 tttgagttgc tcgatgagca gggtggcgtc cgagttgtag tcggggcggc ggaaaatgcg
     3121 ttgcagaatt gacatggatg ggaccttttc cgcaatttta cttccggccg agatgaccgc
     3181 cactttcttg aagatcctgg cccaaggccg ccaggcgtgc ttcacgcgtg ccggcagggg
     3241 tccggtgcga atcgtattag catacgttcc tcccatgaga ccttctgttc gttgcccgag
     3301 agtccccgcc gacgcggacc aaccgggacc ggcaagccgt gcgaggggcc ggcccgtggg
     3361 gcctgaaaga tgacggattt gacaaagtta tggacctgac cggctggctg cggctggact
     3421 ggatactggt ggccgtcgtg ggaggatggc tgctgatcgg cgttgccggc gtgctggggc
     3481 tgcgccgctt caccttcgtc tcgcgtgtgc tgtttccggc cggcggcgcg ctcggcctgc
     3541 tgctgttcgg cctggcgctg ggcgccattt tcggcgcacc ggaagtcgcc gtgctgccca
     3601 tcgggttgcc caacctgccg tttcacctgc ggctcgacag cctctcggcc ttcttcctga
     3661 tggtgatcgg cgccgtttcg gccgggattt cgaccttcgc ggccggctac ttccgcaagg
     3721 cggaaggcac gccccccggc ctgttgtgcc tcgagtacca cgtgttcctc gccagcatgg
     3781 ccctggtggt gctggccgac gatgcctatg ccttcatggt gatgtgggag acgatggccc
     3841 tgtcctccta cttcctggtg accgccaatc accgggtgcc tgaagtccgc cgcgccggct
     3901 atctgtacct gacgatggct cacatcggcg cgatctcgat ccttctgtgc ttcggcgtgc
     3961 tgcaggccaa caccggcgac tacaccttcg acaacatgcg cgcccagaac ctgtcgccgt
     4021 tctgggcttc cgtgggcttc ctgctggcgc tgctggggtt cggcgccaaa gcgggcctgg
     4081 tgccgctgca tgtctggctg cccgaagcgc atcctgccgc gccttcgccg gtatccgcgc
     4141 tgatgagcgg cgtgatgctc aagacggcga tctacggcct gctgcgcgtc tcgttcgacc
     4201 tgttgcagac gcggttctgg tggtggggcg gactgctcct ggcgctgggt ctcgccaccg
     4261 ccctcttcgg cgtcgtgttc gcggccgtgc aggtggacat gaagcggctg ctggcctatt
     4321 cgtcggtcga aaacatcggc ctggtgatcg ccagcatcgg cctggccctg atcttttccg
     4381 ggtacggcat gaaggccctg gcggcactgg cactgacggc cgcgctctat cacgtcgcga
     4441 gccacgcctt cttcaagagc ctgctgttcc tgtgtaccgg ctgtgtcttg catgccaccg
     4501 cggaacgcag cctgggcaaa ctgggcggcc tgatccgcta catgccctgg gtcgcgtggg
     4561 tcacgctcat cggcgtgctg accagcgccg ggctgccccc gttgggcggc ttcgtgtccg
     4621 agtggctgct gctgcaaagt ttccttttca cgccgggcct gcccgatgcg ctgctgaaca
     4681 tgctgatccc cgcggcggcc gcgctcgtcg cgctggtggc cgccctggcg ggctacacca
     4741 tggtcaagtt cttcggggtc atcttcctgg ggcggccgcg cgaggaaaag ctggcgcagg
     4801 cccacgacgc cggtgcctgg gagcgcgtgg ggatgctgtg gctggtgctg ggttgcgtgg
     4861 cgctggggct cgcgccggtg cagttcatcc agatcatcga tccggtgacg cagcagctgc
     4921 tggccgccgg cctcggccgg acggtggccg ccagtggctg gctgctggcg ccgatcagcg
     4981 tcgagcgggc cagctacggg ccggtgattt tcctgattgg tgtcgccgcc agctttgcgc
     5041 tcgccttcct gatggtgcgc tggttctacc acggcaggct gcgtcgctcg ccggcgtggg
     5101 actgcggctt cccgtggcag aacgcacgca tgcaggacac ggcggaaggc ttcggacagc
     5161 cgatccggca gatcttcgag cccttcttcg tgatgcggcg cgagctgccg accccgttcg
     5221 acgcccgccc gcgctaccgc gtcgtggtcg aggatcactt ctggggctgg atctatctgc
     5281 cgctggcggc gctcgccgag cgcattgccc ggctggtcgg cctgctgcag cagggccgca
     5341 tttcgatcta cctgctttac agcttcgtga cgctgatcgc gatgctgctg gtggtgaaac
     5401 gatgaacatc tacggcgtca tttcccaggc gctcgagatc gtggtggccc tggccctcgc
     5461 gccgctgctg accggctggg tcaaccagtg gcgagcctgg ttgcagaaca agtcggctcc
     5521 gagcctgtgg cagccctacc gcgtgctgca caagctgttc aacaaggact cggtggtggc
     5581 cgatcatgcc tcgccgctgt ttcgcgccgt cccgtacatc gtgttcgggt gcatggcgct
     5641 ggccagttcg atcgtcccga ccctctccac ggacctgccg ctcgcgccgg cggccgacgc
     5701 gatcgccctg gtcggccttt tcgcgctggc ccgcgtgttc atttcattgg ccgccatgga
     5761 catcggcacc tccttcggct ccctgggtgc gcgccgcgaa atgctgatcg gcttccttgc
     5821 cgagcccgcg ctgctgatgg tgctgttctc ggcatcgctg atttccaagt ccacttcgct
     5881 gaccaccatg gtggaggcgc tggcccacca ggaactggcg atctatcccg ccctcgcgtt
     5941 cgcgggcgtg gcgttcacca tggtgtcgct ggccgagaac gcccgggtgc cggtcgacaa
     6001 cccggcgacg cacctggaac tcaccatgat ccacgaagcg ctgatcctgg agtactccgg
     6061 ccggcacctc gccctgatcg aatgggcggc cagcctgaag ctgttcgcct actcgtgcat
     6121 cggcctggcc ctgttctttc cctggggcgt ggccgaggcc gacgcgccgc tggccctgct
     6181 gctggcattg ccggtgctgg tgggcaagct ggcgatcggg ggcttcctgt tggccgtgct
     6241 ggaaaccctg gctgccaaga tgcgcatctt ccgcgtgccc gaattcctcg ccggggcctt
     6301 cctgctggcc gtcatcggca tgctcgttca catcctcctc gcgacatgag cgaactcctg
     6361 gcccagttcg tgaacctgct cggcgcattg ctgctgatgc tggcgttcgc catgatctcg
     6421 cagcggcgga tccttacgct catcaacctg ttcacgctgc aagggctcgt cctggtggtg
     6481 tccaccgcgg tggtgggcta cgtgaccggc cagccccacc tgtacctgtc ggcgggactc
     6541 actttcgtgc tgaaggtggt gctgatcccg gtcctgctgc accggatcat cgaccgcctc
     6601 gagatacggt gggatgtcga aacgctgatc aacattccga ccaccatgct gatcggcatc
     6661 ggggtcgtga tcttcgcttt caatctggcg acgccgatcg ccatgctctc gacctcgctc
     6721 gcccgcggca cgctgggcat cgcgttggcc tgcgtgctgc tgtccttcct gatgatgatc
     6781 acccgcgcca aggccgtgcc gcaggtgatc ggcttcctgg cgatggaaaa cggcctgttc
     6841 ttcgcggcca cgtcggccac ctacggcatg ccgatggtgg tggaactggg catcgcgctg
     6901 gacgtgctga tcggtgtgct gatcctgggc gtgttcatgt tccagatccg ggaacagttc
     6961 gacagcctgg acatccggca cctggaaaag ctgaaggacc aataggaaat gggcccccac
     7021 gttcacattc gttctctgcc ccccgagggg gctgaatgcc gcggctccaa gcctgcctgc
     7081 gcaggcttgg acggcatgaa gagcagccgc gtctcgcggc ggggcggtct gcaagactca
     7141 gcctccttga ggcggctcgg cggagtctga gatggaaacg ctgttcgtcg ttctggggat
     7201 cccgctggcc ggtggcatca cgctggccct gctcgggcat cgcgacgcgg cccgggacat
     7261 caacgtggcg ttcagcctcg gcacgttcat cgcggcctgc ttcctcacgg cccggatcgt
     7321 cgcagacggc ccgatgttcg tctggaacga ggaattcttc atcgatccgc tgaacgtgtt
     7381 cctggtcacg ctcacggcat tcgtcggcct gaccaccgcg attttttcgc gcccctacat
     7441 gcgggtcgag cgcgaccacg gcaagatgac gcccaaccgg atgcgcctgt accacagcat
     7501 gtaccagctg ttcagcttca cgatgctgct ggccctgatg accaacaaca tgggcatcct
     7561 ctgggtggcg atggaggcgg ccacgctcac caccgtgctg ctggtcagcg tctaccgcac
     7621 ggccgccagc ctggaggcgg cgtggaaata cttcatcctg tgcggtgtcg gcatcgcgca
     7681 ggcgctgttc gggaccgtac tgctgtacat ggcggcggag aaagtcctcg gctcggaagg
     7741 cggcgcgctg ctgtggaccc acctggacgc ggtgaaggac cggctcgacc ccaacatcat
     7801 gaccctggcc ttcgccttcc tgttcatcgg ctacggcacc aaggtcggac tggtgcccct
     7861 gcacaactgg ctgcccgatg cgcatgccga agggccgacg ccggtatcgg ccgtgctgtc
     7921 cgggctgctg ctgaatgtgg cgctctatgc ggtgctgcgc tgcaaggtgc tgaccgacgg
     7981 cgcgctgcaa agccatctgg ccggcaacct gatgatgggt ttcggcctgg tgtcggtggt
     8041 cgcctcggta ttcctgctga tccggcaaaa agacgtcaag cgcatgttcg cctactcgtc
     8101 gatcgagcac atggggctga tgacgttcgc tttcggaatg ggcggaccga tcgccaattt
     8161 cgccggcctg ctgcatatga ccgtgcactc gctggtcaag tccgccatct tcttcgccgt
     8221 cggccacgcg gcgcagaagg ccggcacgca gatgatggac gcgatccgcg gcctgatcaa
     8281 ggtcagcccc acggtcggat ggggcttgat gctgggatcg ctggccattc tgggaatgcc
     8341 gcccttcggt gtttttgcca gcgagttcct gatcgtgacc acggccatgc gcgagcagcc
     8401 ctgggcgacg ccgttcctgc tggtggcgct cggcgttgcc tttgcctcgg tgttcagccg
     8461 ggtccagccg atggtcttcg gcgacaccag cgtcaaaccc ctggcgcacc cgccggcgct
     8521 gatcccggtg ttcgtgcacc tggggctcgc cttgatgctg ggtctgtaca tcccgccgta
     8581 ccttgacatg tggtaccgcc aggcggcccg aatgctgggg aactgacgtg gcgacgatcg
     8641 aagccttgaa cttccagctc gaacgcctgc cggcgccgtt gccgctgtgg cgcgcgggtg
     8701 tcgatgcgtt ccaatgggtc gcggcggcgc gggccatggc gcaggcgggc ggccggttgg
     8761 tgtcgctgtg gggctgcgac cgttcgcttg gcccggccac ggcgctggcc gtgtcggcgg
     8821 cctacgccgt caaggagggc atggtctggc tcgatctggc actgggcgaa cgtgatacgg
     8881 cgtatcccga cctcgccggt atcttcccgt tcgcggcccg catgcagcgc gcgacggccg
     8941 atctgctggg gatcgcggcc cagggctcgg aagatggcag gccctggctg aaccacggcg
     9001 cctggcccga aggctatttc ccgctgcgcc gggatgccac gtcgggcgtc ggctccatga
     9061 gtccgcagcc ggtgcgggac tatccgttcg tgccggtcga aggcgacggc gtccacgaaa
     9121 tcccggtggg gccggtccat gccggcatca tcgagcccgg tcatttccgt ttctcggtgg
     9181 tcggcgagaa ggtgctgcgg ctggagcagc gcctgggcta cacccacaag ggaatcgagc
     9241 agcggttcac gcggctcgcg ccgctggaag cccacaagct cgcggggcgc gtttcgggcg
     9301 atagcacggt ggccttcgcg tgggcttatt gcatggcgct cgaatcggcg gcgggctgcg
     9361 acgtgccgga acgtgcgcaa tggctgcgcg cgctgctgct cgagcgcgaa cgggttgcca
     9421 accacctggg cgacctgggc gcgctgggca acgacgcggc gctggcgttc gggctggcgc
     9481 agttctcgcg cctgcgcgag gactggctgc gcctgtcggg cgaggcattc ggccaccgcc
     9541 tgatgatgga ccgtgtcgtg cctggaggcg tcgcggggga cgtggacgcg gccatgcgcc
     9601 ggcgcctgca acaccaatgc gatgccatcg aggccgaggt gcgcgcgctg gaagcgatct
     9661 acgagtcgca cgccgggctg caggaccgct ttgcgaccac cggcatcgtg gcgccgcaac
     9721 tggcggcgca actgggcctg gccggcctgg cgggccgtgc cagcgggcag ccggcggatc
     9781 ttcgctgcga ccagtcctgg gcgccttatg agcgtctgcg cgtcaaggcg gccgtccgcc
     9841 agaatggcga tgtcgccgcg cgggtcgccg tccggttcga ggagaccctg gagtcgttgc
     9901 gcctgatccg cgagatctgc cacagcctgc ccgagggccc cttgcgaggc gccgtcgagt
     9961 tgccgcggaa ggccgcgaca ggcgccggct ggatcgaagg ctggcgcggc gaggttttcg
    10021 tggcgctgga actcggcgcc gatgcgggca ttcgccgctg ccattgccac gacccttcgt
    10081 ggcagaactg gcccgtgctc gaacacgccg tgatcggaaa tatcgtgccg gacttcccgc
    10141 tgatcaacaa gtcttttaac ctgagctact cgggacacga tctgtagatg aaagcccacc
    10201 cccgaagcgc gatggatcac tgacatgtgg aaaatcgtcc gacagatcgc gcgcgccggc
    10261 attcgcaccg aggcgccgcc attcgcaacg ggtgaggact cgccggttgc ggtggagcgg
    10321 atccagaagg aaattctcga catcctgggc caagccctgg ccatccggca ggtggacgcc
    10381 ggttcctgca acggctgcga gctggaaatc cacgcgctca acaaccccta ctacaacctg
    10441 gagggcctgg gcatccggtt cgtcgcgagc ccgcgccacg ccgacatgct actggtgacc
    10501 ggcccggtct cgcgcaacat ggagatggcg ctgcggcgca cctacgaagc aactccggag
    10561 ccccggctgg tggtggccgt cggcgattgc ggctgcaccg gcggcatctt cggcgagagc
    10621 tacgccacct gcggcgcggt ggccaacgtg ctgcccgtgg acgcgacggt gccgggctgc
    10681 ccgccgccgc cgatcgagat catgcgcggc atcctcagcg cggtccgtcg ccgccgctag
    10741 cccgtctttc tcaactgacc agccgaaacg ccgctaaccg gttgattttg ctggtgaggg
    10801 accccgtagg tcttcactac agggtggctg cgggcggctt cctttgcggc acctgggcgg
    10861 ccgagatctt cggcggtggc tgctcgggca ggcgcagctt gagcaggtcc agcagcatgc
    10921 ggtgttcggc ctcgggctgg gtgtagcgag acagcaccag ctcgcgcccg tcgctggtgg
    10981 gcaagtgcac gtccaccatc tgcatggcct tgaacttctc ggtcacgctg cgcggggtca
    11041 gcccctgggc gtgctggcgc aggttggcct tgagcgtgac ctgcaggcag taggccagga
    11101 aggccacgaa gatgtgtgcc tcgatgcggt gttccagctg gtggtagatc ggccgcagcg
    11161 ccaagtcgtg tttgagttcc ttgaatgcct gctccacctc ggtgagctgg atgtagaagc
    11221 tccacagctg ctcgggcgcg ccggcgccga ggttggtgcg aagcaggtag cggccctcgc
    11281 gctggcgcac ccggcgcagc ttgtggcgat cgagcttgaa ttcgaagctg gccgtgcctt
    11341 cgctggcctt gggcaccgat accttcacca cgctggcgac gcgccccgcg tcgtgcttgg
    11401 ccgcccccag cttcatgagc agctggtcac gggtgagcac ctgctgctgc aacgctttga
    11461 ggcgctgcca atagcggcgc agccgcttgc gccgcatggc gcgctccttg tcgatgcgcg
    11521 catcgctttg cgccaggatg tacacgtcct cttggcaggc cagctgcttg acctgcacgc
    11581 cttcgcgcac cttggcccag ggttgttcca gcagccgctg ctccagcttg ttcagccgtc
    11641 ccttgggcgt tcccaccagg taggacgcgc ccgcagcgcg catcgcctgc aggctgtcct
    11701 cggtggggat gccgcggtcc atcacccaga tgcggttggc ccgcccatag cgctgctcga
    11761 tgcgagcgag catgccgctc aaggtggtcg agtcggcggt gttgcccgcc agcacctcgt
    11821 agctcagcgg aaacccctcg ggtgtcacga tcagcgcgat caccacctgg cggcagtcgc
    11881 ctcgcttgtc gcggctgtag ccgtattggc gcagatcgcc gctgctgcgc tcttcgtcgg
    11941 tctcgaagta ggtgctggtc aggtcgtaca gcagcacttc gaagctcgcg ccaaacagtt
    12001 caccccagcg ctgcttgagg aacttgtact gctcgtcctt gtgcgcggca agtttgtcca
    12061 ggcagcggta cagcgtgttc ttctcggcca gctcgaagtc cgcctccagc agatcggcca
    12121 tggcgctggc gtcaaaccac tggcggtgca gccgccactc gctgcccggg tcgatcagcc
    12181 ggtaggccac cagcgtcttc aggaccttca gccacggcgt gccttcgcgc gagggcacca
    12241 gccgaggcgc ccagaaggca tccaggtcaa gctgctgcca cagctgcatg gccagccagc
    12301 acgcgcccca ctggcgcggc cgctcaaggc gcaactcgcc cactcgcacc tcgatcgcat
    12361 cggccgcgtc ggcgggcatg ctgcccaccg gaaacagcgc cacctggcgg cgcgcgcttc
    12421 catcatgcac ttcaacggtc ttgcgccagg cttcgcgctg gctggcattg atctcgccca
    12481 gatacagcac ctggcgctgc accacgcgcc cgtcggccag gcggcggttc tccaccacgt
    12541 tccagtactg gtggaccttg ccatccttct tgcggcggct gcagcgcaaa aacatgcgcc
    12601 gcactctatg cgcgcacccg gcacgccgca aggaccaagg tcttcactac aagcgttttg
    12661 cgaaaacgct cctatacgca tcaacggctt acgagccgtt taagggccga ttcccaggaa
    12721 aacaacggcg agttgagaaa gacgggctaa gggtggggtt cactgacgtc cctgttccgc
    12781 aaatatcatc gagcgctacc ggtgcgaatt tcggccattc gtgatcactc attccaacag
    12841 ttcttggtca ggcagttcgg catcgtgatc accggcaggg aaacaccact tcttgatgcg
    12901 gattcaaccg gtcgttcgag ccgcgttttc tgatctccgc tgtcggcgtg gccggtggtt
    12961 gaaaggaaat caggctcacg atgcacttgg ctcgatcgcc gcaggcatga gcgccagcag
    13021 ggcgcgcttc aagcctgcgc ggcgctgggg ctctcgacga agacgcagcg tttacctgtt
    13081 cgcttgcaat aatctttcac gcgccaatag gcgtcatggc taatgagtcg cacgaaatct
    13141 agaaactgat caatgcccct ttgcgccttt gcgcccgaaa gctgatcaag acaaagttca
    13201 gtgtcatccg tatttcggat atttgcgcaa tagccgtatt tgggatattg ccaaaatatc
    13261 tgagaagcag ctataatgcg aatagctgaa aatcggatac gtcatatgtc tctccccccc
    13321 cccaccccgc ccc
//
LOCUS       DASWIY010000238         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2383467, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000238 DASWIY010000000
VERSION     DASWIY010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2383467"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VGA59_08805"
     CDS             complement(<1..691)
                     /locus_tag="VGA59_08805"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9719816.1"
                     /translation="MPDDHVPPMKLSGLEPVTIGAAPSSADANAGEGPSPTATFVNIG
                     ERTNVTGSKAFARMILNGQFEEALAVARQQVENGAQVIDINMDEAMLDSKASMVRFLQ
                     LIASEPDIARVPIMVDSSKWEVIEAGLRCIQGKGIVNSISMKEGVEAFKHQAKLVKRY
                     GAAAVVMAFDEKGQADTFKRKTEISARAYRILTEEIGFPAEDIIFDPNIFAIATGIEE
                     HNNYGVDFIDAT"
     regulatory      complement(731..807)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            820..1455
                     /locus_tag="VGA59_08810"
     CDS             820..1455
                     /locus_tag="VGA59_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9719817.1"
                     /translation="MHRANAIHRPVSGMVSRLFGKKAAAADKGLARQRLHKIAGSSAA
                     DLAAEMLSAPSALLQLNHEEARTVVAYMLPHKIAEGTTFIREGDTAHTDFMLLVLDGE
                     VTIETIVVSRTEPITVTVLGPGSLIGEMGVLDGAPRSASCTAISDLRCAILTRDAMSQ
                     LLDDEPRTAAKLMMAISLRIAQRMRENQDKLKLYAQLTQAMNEEINALMPL"
     gene            1538..2530
                     /locus_tag="VGA59_08815"
     CDS             1538..2530
                     /locus_tag="VGA59_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HEX9719818.1"
                     /translation="MAFPTWTRALRAFGISFALGVSLLGGPALAQGSSTPIRILVGFP
                     PGGGTDAIARTLADKLKDQLNQPVLVENRAGAGGQIAAQALKAAPADGHTLFISHDHT
                     ISILPLVTKNPGYDPAKDFVAVAGFATFVNALAVSGGTPARTFPEYLAWVRQKGGGKG
                     NIGIPAPASVPEFLVKLIGQKYALDLQSAPYRGSAPMIGDMLGNQIGAGVGSIPDFVE
                     NHKAGKIRVVAVMGNARQAAMPDVPTFAEFGLAGFEDVPYYGLFAPAGTPQAAIDRFG
                     EALAKVLAQPDVRERLTAMGLTVGFMPQQQLASRERAYTKTWTRIIKESGFQAK"
     gene            2921..>3094
                     /locus_tag="VGA59_08820"
     CDS             2921..>3094
                     /locus_tag="VGA59_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX9719819.1"
                     /translation="MIRLELQLELAYEIRDSDGADFVFNIQAAQTPHQTVEDERLVVN
                     QAVTPEVATDPATA"
BASE COUNT          529 a         1019 c         1009 g          537 t
ORIGIN      
        1 gtgtcgcatc gatgaagtcg acgccgtaat tgttgtgctc ctcgatgccg gtcgctatgg
       61 cgaagatgtt tgggtcgaag atgatgtcct cggcgggaaa cccgatctcc tcggtgagga
      121 tgcggtaggc gcgggccgaa atctcggtct tccgcttgaa ggtgtcggcc tggcccttct
      181 cgtcgaaggc catgaccacg gcggccgcgc cataacgctt gaccagcttc gcctggtgct
      241 tgaaggcttc cacgccttcc ttcatggaga tcgaattgac gatgcccttg ccctggatgc
      301 agcgcaggcc ggcctcgatc acctcccatt tggacgaatc caccatgatc ggcacgcggg
      361 cgatgtccgg ctcgctggcg atcagctgca ggaaacgcac catggaagcc ttgctgtcca
      421 gcatggcttc gtccatgttg atgtcgatca cctgtgcgcc gttttccacc tgctggcgcg
      481 ccacggccag ggcttcctcg aactggccgt tgaggatcat ccgggcgaag gccttggagc
      541 cggtgacgtt ggtgcgctcg ccgatattca cgaaagtcgc cgtgggggac gggccttcgc
      601 ctgcgttggc gtccgctgaa ctgggggcgg caccgatggt gacgggctcg aggcccgaga
      661 gtttcatcgg gggcacatgg tcatcgggca ttggctcgtc ctgcatgggc cggcacgcgc
      721 cggcggtggg gggaggtgag cgccgttggg tcctcaagcc tggcggctta agcaaattgc
      781 ctagccgctg cagcgctcct tgagctagca tgattctaaa tgcaccgcgc caatgccatc
      841 catcgccctg tgtccggcat ggtcagccgg ctgttcggga aaaaggccgc ggcggcggac
      901 aagggccttg cgcggcagcg gctgcacaag atcgcgggct cgtcggcagc cgacctggcc
      961 gccgaaatgc tgtccgcccc cagcgcattg ctgcagctga accacgaaga ggcacgcacc
     1021 gtcgtggcct acatgctgcc gcacaagatc gcggagggca ccaccttcat cagggagggc
     1081 gacaccgccc acaccgactt catgctgctg gtgctggatg gtgaagtcac gatagagacc
     1141 atcgtggtca gccgcaccga gccgatcacg gtgacggtgc tcgggcccgg cagcctgatc
     1201 ggcgagatgg gggtcctcga cggcgcgccg cgttcggcct cctgcacggc gatatccgac
     1261 ctgcgttgcg ccatcctcac gcgcgacgcg atgagccagt tgctcgacga cgagccgcgc
     1321 accgccgcca agctgatgat ggcgatctcg ctgcgcatcg cccagcgcat gcgcgaaaac
     1381 caggacaagt tgaagctcta cgcgcagctg acgcaggcga tgaacgaaga gatcaatgcg
     1441 ctgatgccgc tctgacggcc aggcgtggcc ggtcgggtta cgcgcccgcc gatttgcaac
     1501 aatggaggcc ccccgtcttt tcgaggagtc ctccaccatg gcatttccca cctggacccg
     1561 cgccctgcgc gccttcggca tctcgttcgc gctcggcgtg tcattgctgg gcggccccgc
     1621 gctggcccag ggcagcagca ctccgattcg catcctggtg ggctttccgc ccgggggcgg
     1681 caccgatgcc atcgcccgga ccctggcgga caaactgaaa gaccagctga accagccggt
     1741 gctggtggaa aaccgtgccg gcgcgggcgg ccagatcgcg gcgcaggcgc tcaaggccgc
     1801 cccggccgac ggccacacgc tgtttatctc gcacgaccac accatctcca tcctgccgct
     1861 ggtcacgaag aaccccggct acgatccggc gaaggacttc gttgccgtgg cgggcttcgc
     1921 caccttcgtg aacgcgctgg cggtctccgg cggcacgccc gcccgcactt tccccgagta
     1981 cctggcctgg gtgcgccaga agggcggcgg caagggcaac atcggcatcc cggccccggc
     2041 atcggtcccg gaattcctgg tcaagctgat cgggcagaaa tacgcgctcg acctgcagtc
     2101 cgcgccttac cgcggcagcg ctcccatgat cggggacatg ctgggcaacc agatcggcgc
     2161 cggcgtcggc tcgatccccg atttcgtcga gaaccacaag gccggcaaga tcagggtcgt
     2221 ggccgtgatg ggcaatgcgc gacaggcggc aatgcccgac gtgcccacct tcgccgaatt
     2281 cgggctggcg ggcttcgagg acgtgcccta ttacggcctg ttcgccccgg ccggcacgcc
     2341 gcaggcggcg atcgaccggt ttggcgaggc gctggccaag gtgcttgcgc agccggacgt
     2401 gcgcgagcgc ctcaccgcca tgggcctcac ggtgggcttc atgccgcagc agcagctggc
     2461 cagccgcgag cgcgcctata ccaagacctg gacccgcatc atcaaggaaa gcggctttca
     2521 ggcgaagtaa gcgcacggat cgggcgcccc gccggacgca cgacgaacat cagccgggag
     2581 tcatcctcga tcccgcagcg tttgactttc gtggcggcgt tcttgttttg atcgcgcagc
     2641 tcgctttagc agtgtctgag gcggcgttta tcaagaagtg cttgcgcgac aggacgaaga
     2701 agccgtgcgc ggcggcgccg aagcacgggt ggggcattag ctgcagcgcc ctccctcgcg
     2761 aatgacctgc tccactgtct gatggccgct gtcggccaga agcggctatt gctgctggac
     2821 tcggtcgctt tgatcccccg aacaacgtcg gtgacggata atatcgggca acgccgcgcc
     2881 ctccgggcga cggaacccga tatgaggagc atgctctcca atgatccggc ttgagcttca
     2941 gctggaactc gcttacgaaa ttcgggattc cgatggggcc gatttcgtat tcaacattca
     3001 agccgcccag accccgcacc aaacagtgga agatgagcgc ctggtcgtga accaggcggt
     3061 cacgccggag gtggccactg acccggcaac cgcg
//
LOCUS       DASWIY010000239         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_142485, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000239 DASWIY010000000
VERSION     DASWIY010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142485"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VGA59_08825"
     CDS             complement(<1..296)
                     /locus_tag="VGA59_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719820.1"
                     /translation="MSTGTESPQGRAAPSPPPEGVKETWGGPAFPCESNAAASGGAAW
                     IAALQRNLELREGAVSVAQTHISWVLLTPQFAYKIKKPVHLSFLDFSTLARRKH"
     gene            complement(280..>1898)
                     /locus_tag="VGA59_08830"
     CDS             complement(280..>1898)
                     /locus_tag="VGA59_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9719821.1"
                     /translation="PGKQEELAESAFRKFLTWTLFTQSALPGTPLKSLSSVPSLPEDK
                     RFAPEQWTAPGYHQLAQAFLLTQQWWDEATRGVRGVSQHHEEVTEFMTRQWLDMWSPS
                     NFVATSPQVQQEVWSTGGLNLAYGVANWWRDAIAVLTDGAPAGAERFEPGETVAVTPG
                     KVVFRNRLIELIQYEPATRTVFPEPVLIVPSWIMKYYILDLSPDNSMVKYLVDKGHTV
                     FMISWKNPDASDRDLRMEDYVELGVFAALKAVEAAMPGHGIHATGYCLGGTLLAIAAA
                     AMAHDESSPLKTLGILAGQTDFQEPGELGLFIDESQIAFLEDLMAQRGYLDGRQMAGA
                     FALINSKDLVWSKLVHEYLMGAHTPLTDLRAWNADATRMPHRMHSEYLRRLYLGNDLA
                     EGRFPVRGRPVAINDIHLPTFVAATERDHVSPWKSVYKINLLTRTEISFVLTAGGHNV
                     GVVNPPEGPQAHPSASHRFAVREAGAPYVDPQTWLDSAPQQSGSWWTTWQHWLAEHSG
                     KPVPAVPVGRRGKAHVPSLGDAPGSYVHGN"
BASE COUNT          281 a          634 c          658 g          325 t
ORIGIN      
        1 aaatgcttgc gtcgtgcaag cgtgctgaag tcgagaaagc tcaggtgcac cggcttcttg
       61 atcttgtagg cgaactgcgg tgtgagcagc acccaggaga tgtgcgtctg cgcaacgctg
      121 accgcacctt cgcgcagttc caggtttctt tgcagcgccg ctatccacgc ggcgccgccg
      181 gatgcggccg cgttgctctc gcaaggaaac gccgggccgc cccaagtttc cttgaccccc
      241 tcggggggcg ggctgggcgc agcccggccc tgggggctct cagttcccgt ggacatagct
      301 gcccggcgca tcgcccaacg agggcacgtg cgccttgccg cgccggccca cgggcacggc
      361 cggcaccggc ttgcccgagt gctcggccag ccagtgctgc caggtggtcc accatgaacc
      421 cgactgctgc ggcgccgagt ccagccaggt ttgcggatcg acgtagggcg caccggcctc
      481 gcgcaccgca aagcggtggc tcgccgacgg gtgcgcctgc ggcccctcgg gcgggttgac
      541 gacgcccacg ttgtggccgc cggcggtgag cacgaagctg atctcggtgc gcgtgagcag
      601 gttgatcttg tagaccgatt tccacggcga gacgtggtcg cgctcggtcg cggccacgaa
      661 ggtgggcagg tggatgtcgt tgatcgccac cggccggccg cgcaccggga agcggccttc
      721 ggccaggtcg ttgccgaggt agaggcggcg caggtattcg ctgtgcatgc ggtgcggcat
      781 gcgcgttgcg tcggcgttcc aggcgcgcag gtcggtcagc ggcgtgtgcg cgcccatcag
      841 gtactcgtgc acgagcttgg accacaccag gtccttggaa ttgatgagcg cgaacgcgcc
      901 ggccatctgg cgcccgtcga ggtagccgcg ctgcgccatc aggtcttcca ggaaggcgat
      961 ctggctctcg tcgatgaaca ggcccagttc gcccggctcc tggaagtcgg tctgcccggc
     1021 caggatgccc agggttttca gcggcgaact ttcgtcgtgc gccatcgcgg ccgcggcgat
     1081 cgcgagcagc gtgccgccca ggcagtagcc ggtggcgtgt atgccatggc cgggcatcgc
     1141 ggcctccacc gccttgagcg cggcgaacac gcccagctcg acgtagtctt ccatgcgcag
     1201 gtcgcggtcg ctcgcgtcgg ggttcttcca cgagatcatg aacaccgtgt ggcccttgtc
     1261 gaccaggtac ttgaccatcg agttgtcggg cgacaggtcg aggatgtagt acttcatgat
     1321 ccacgagggc acgatcagca ccggctcggg aaacaccgtg cgcgtggccg gctcgtactg
     1381 gatcagctcg atcagccggt tgcgaaacac caccttgccc ggcgtgacgg ccaccgtctc
     1441 gcccggctcg aagcgttcgg cgcccgcggg tgcgccgtcg gtgagcaccg cgatcgcatc
     1501 gcgccaccag ttggcaacgc cgtaggcgag gttcaacccg ccggtggacc acacctcctg
     1561 ctgcacctgc gggctggtgg ccacgaaatt ggacggcgac cacatgtcca gccattggcg
     1621 ggtcatgaac tcggtcactt cctcgtggtg ctgcgacacg ccgcgcacgc cgcgcgtggc
     1681 ctcgtcccac cattgctgcg tgagcaggaa ggcctgcgcc aactggtggt agcccggcgc
     1741 cgtccattgc tcgggcgcaa agcgtttgtc ctccggcaag gacggcacgc tgctgaggga
     1801 cttgaggggc gtgcccggca gcgccgattg cgtgaacagc gtccacgtca ggaacttgcg
     1861 gaaagcgctc tcggccagtt cctcctgctt gccgggcg
//
LOCUS       DASWIY010000240         3948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1643528, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000240 DASWIY010000000
VERSION     DASWIY010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643528"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..787
                     /locus_tag="VGA59_08835"
     CDS             35..787
                     /locus_tag="VGA59_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719822.1"
                     /translation="MSALRKVLVVDDDPVVGKSFNRVLSDKGYIVVTAEDGYDALMKL
                     QAEKYDVVYTDIKMPGMDGLEVAEQVKARQPWTPVVIVTGYGSAANKERARAAGVTDF
                     LHKPLSPEMIEDSAANALRQAFVPPVVAIPRSKEVAAPAPAPAPAAAEAPNEGSVLKN
                     MALFLSAPFIGLLYAILLPFVGLGMLGWFAAQALLEKARDRDLVRFGTFALRLLAGPF
                     IGLAFLIVLPFAAMGMLAWTGARALVERVPTE"
     gene            837..2231
                     /locus_tag="VGA59_08840"
     CDS             837..2231
                     /locus_tag="VGA59_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX9719823.1"
                     /translation="MTRSRKTGETADLPHRSRALWGAVAAVAFSLLGLAAQAAGPKDA
                     TLSEEDKVCLGCHAKPDLVKTLGDGQKLSLVVPARGFAQSVHNSSGCEACHSDIDLKG
                     HVNEPRSIASKRAFALEQTETCRDCHKKTAKQYDDSVHSALVKTGSDKAPVCSDCHNP
                     HATRSAKEKPSGAVQPVACNNCHESIATAYADSVHGRSGDEALACKDCHQTHNVKAAA
                     LGDHVKKECMACHRDVSSTHSAWLPNTERHLEAISCPACHSPGTTRRVNLRLFEGNTP
                     HQAENVGVPKFVKLANSADGTASGLDARALWSLLQEFNSDGEERKTVVRGRLEVQTGV
                     QAHQLGERSLAVKDCDTCHRQGAVSFQAVTVSMAGPDGRPLRHEASKGILNSIESVGS
                     VGGFYAIGSTRIKLLDTMLVMALAVGILIPTAHLTAKVVTRRRRAQQAHADTAAASSD
                     NSQAAGNEPRTGAE"
     gene            2247..2915
                     /locus_tag="VGA59_08845"
     CDS             2247..2915
                     /locus_tag="VGA59_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX9719824.1"
                     /translation="MSKIYVNPLPVRIWHWTNAVLFIVLIATGVQIRYLDLFGFMSFK
                     NAVTVHNWTAFALIANFFVWLLFYLFSDKNKAYHPEFNIIKLANASMRQAVYYGWGMF
                     RGEANPHKIDPYLKFNPLQRTLYQILMLFLLPMQFVTGVLLWDVKRFSNVIEMAGGVR
                     VIDTVHVLIFIFFVFYMLFHPYLATLGHTPTAHIKAMITGYEDVDDEPEEPTQSASAV
                     ARIV"
     gene            2980..>3948
                     /locus_tag="VGA59_08850"
     CDS             2980..>3948
                     /locus_tag="VGA59_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9719825.1"
                     /translation="MKSNSKTIVAAVCGALLLGPALIGGAAIAADAPAAQSAQAQAAR
                     FPKRVDATFVKQYAVMPKPEGVTIVDSRPAARKYDVGHIPTAVNIPDLQFDKLAATLL
                     PQDKSQLVIFYCDGPECSLSHNSAFKAEKLGYTNVRVYAEGFPDWIGKGNLHAVSIAQ
                     VKKLLNEKAPLTLVDARPKERKFDKGHIPGAISLPDSQFDKLAAERLPADKAAALYFY
                     CDGLACKLSNDSAEKAIKLGYTNVKVVPEGYPAWEKAYGPNPGAAAAAAPAAAPAPAI
                     VAGKEPGSIAIASFERIYREAPGSVHLIDVREPREFAAGTFKGAINM"
BASE COUNT          751 a         1281 c         1263 g          653 t
ORIGIN      
        1 cttccgataa gcctgatttt ctgaggaaat catcatgagt gcgcttcgta aagtcttggt
       61 cgtcgatgac gacccggtgg ttggcaagag cttcaaccgc gttctttccg acaagggcta
      121 catcgtggtc accgccgagg atggctatga cgcgctgatg aagcttcagg ccgaaaaata
      181 cgatgtcgtc tacaccgaca tcaagatgcc cggcatggac ggcctggaag tggccgaaca
      241 ggtcaaggcc cgccagccgt ggacgccggt cgtcatcgtc accggttacg gctcggcggc
      301 gaacaaggaa agggcgcgtg ccgcgggcgt gacggacttc ctgcacaagc ccttgtcgcc
      361 cgagatgatc gaggacagcg cggccaacgc cctgaggcag gcctttgtgc cgcctgtggt
      421 ggccatccca cgcagcaagg aggtcgcggc accagcgccg gcccccgcgc ccgcagccgc
      481 cgaggcgccg aacgagggga gcgtgctgaa gaacatggcg ctgttcctgt cggctccgtt
      541 catcggcctg ctgtacgcga tcctgctgcc tttcgtcggt cttggcatgc tcggctggtt
      601 cgccgcgcag gcgctgctgg agaaggccag ggaccgcgac ctggttcgct tcgggacctt
      661 cgccctgcgc ttgctcgccg gtcccttcat cggccttgcc ttcctgatcg tgcttccgtt
      721 tgccgccatg ggaatgctgg cgtggaccgg tgccagggcg cttgtggaac gcgtgccgac
      781 ggagtgacga agaacgagcg cgcatcatcg aaactttcga ggaactagag gacgccatga
      841 cacgctcccg caagacaggt gaaacggcgg atctgccaca ccgcagcagg gccctttggg
      901 gcgccgtcgc ggcggtggca ttttccctgc tgggcctcgc cgcccaggcc gccggcccca
      961 aggacgccac cctgtcggaa gaagacaagg tgtgcctcgg ctgccatgcc aaaccggacc
     1021 tcgtgaagac actgggagac ggccagaaac tttcgctcgt cgtgccggcc aggggctttg
     1081 cccaatcggt gcacaattcc agcggctgcg aggcttgcca ttcggacatc gacctgaagg
     1141 gccatgtcaa cgaacccagg agcattgcga gcaagcgcgc ctttgcgctc gagcagacgg
     1201 aaacctgccg tgactgccac aagaagacgg ccaagcaata cgacgacagc gtgcactccg
     1261 ccctcgtgaa aacgggcagc gacaaggcac ccgtgtgctc cgactgccac aacccgcacg
     1321 cgacccgctc cgccaaggaa aagccgagcg gcgccgtcca gcctgtggcg tgcaacaact
     1381 gccacgaaag catcgccacg gcgtatgccg acagcgtgca cgggcgctcc ggggacgaag
     1441 ccctggcatg caaggactgc caccagacgc acaacgtcaa ggcggcggcc ctgggtgacc
     1501 acgtgaagaa agagtgcatg gcctgccacc gcgatgtgag ttcgacccac agcgcgtggc
     1561 tgcccaatac cgagcggcat ctggaggcga tctcctgccc ggcgtgccat tcaccgggca
     1621 cgacgcgcag ggtcaacttg aggctgttcg agggaaacac accgcaccag gcggaaaatg
     1681 tcggcgtgcc gaagttcgtg aaactggcga actcggccga tgggacggct tccggcctgg
     1741 acgcgcgcgc gttgtggagc ctgctgcagg aattcaacag cgatggcgag gaacgcaaga
     1801 cggttgtgcg cgggcgcctg gaagtgcaaa ccggcgtcca ggcacaccag ctgggtgaac
     1861 gctcgctggc cgtcaaggac tgcgacacct gccaccgcca gggcgcggtc tcgtttcagg
     1921 ccgtgacggt cagcatggcc gggccggacg gcaggccgct gcgccatgaa gccagcaagg
     1981 gaatactgaa ttcgatcgaa tcggtcggct ccgtgggcgg gttctatgcc atcggcagca
     2041 cgcggatcaa attgctggac acgatgctgg tcatggccct cgcggtcggc atcctgatcc
     2101 cgacggcgca cttgaccgcc aaggtggtga cccggcgcag gcgggcgcag caagcgcacg
     2161 cggacaccgc agcagcttca tccgacaaca gccaggccgc gggcaatgag cctcgtaccg
     2221 gcgccgagtg aatccaggag atggccatga gcaagattta tgtcaacccg cttcccgtgc
     2281 gcatctggca ctggaccaac gcggtcctgt tcatcgtgct gatcgcgacg ggcgttcaga
     2341 tccgctacct ggacctgttc ggcttcatgt cgttcaagaa tgccgtcacc gtccacaact
     2401 ggaccgcgtt cgccctgatc gccaacttct tcgtgtggct gctcttctac ctgttctccg
     2461 acaagaacaa ggcctaccac ccggagttca acatcatcaa gctggcgaac gcctcgatgc
     2521 gccaggcggt gtattacggc tggggcatgt tccggggcga ggccaacccg cacaagatcg
     2581 acccgtacct caagttcaac ccgctgcagc gcacgctgta ccagatcctg atgctgttcc
     2641 tgctgccgat gcagttcgtg accggcgtgc tgctgtggga cgtgaagcgg ttctcgaatg
     2701 tgatcgagat ggcgggcggc gtgcgcgtga tcgacacggt gcacgtgctg attttcatct
     2761 tcttcgtctt ttacatgctg ttccacccct acctggcgac gctggggcac accccgacgg
     2821 cgcacatcaa ggcgatgatc accggctacg aagacgtcga cgacgagcct gaagagccca
     2881 cgcagagcgc atcggccgtt gcccgaattg tgtgatgaca gcggcgcgca ggccagccgc
     2941 cggctgaagc gcgccttcga tttttttagg aggtcgttga tgaagtccaa ttcgaaaacc
     3001 atcgtcgccg cagtgtgcgg cgccttgctg ctgggccccg ccctgatcgg gggtgccgcg
     3061 attgcggcgg acgcgcccgc ggcccagagc gcgcaggccc aggccgcccg cttccccaaa
     3121 cgcgtcgatg cgactttcgt gaagcagtac gccgtgatgc cgaaacccga gggggtgacc
     3181 atcgtcgact ccagacccgc ggcgcgcaag tacgacgtcg gccatatccc gacggcggtg
     3241 aacatccccg atcttcaatt cgacaaactg gcggccacgc tgctgccgca ggacaagtcg
     3301 cagctggtga tcttctattg cgacggcccc gaatgctcgc tcagccacaa ctccgcgttc
     3361 aaggccgaaa agctgggcta caccaacgtg cgcgtgtacg ccgagggttt cccggattgg
     3421 atcggcaaag gcaacctgca tgcggtgagc atcgcccagg tcaagaagct gctgaacgag
     3481 aaggcgccgc tgaccctggt cgacgcgcgg cccaaggagc gcaagttcga caagggccac
     3541 attcccggcg cgatcagcct tcccgattcg cagttcgaca agctggccgc cgagcggctt
     3601 cccgccgaca aggccgccgc gctgtacttc tattgcgatg gcctggcttg caagctgagc
     3661 aacgactcgg ccgagaaggc gatcaagctg ggctacacca acgtcaaggt cgtccccgag
     3721 ggctatcccg cctgggaaaa ggcctatggg ccgaatccgg gtgcggccgc cgcagcggcg
     3781 ccggcagcgg caccggcgcc ggccatagtg gccggcaagg agccggggtc gatcgcgatc
     3841 gcctctttcg agcgcatcta ccgcgaggca cccggctcgg tgcacctgat cgacgtgcgg
     3901 gagccgcgcg agttcgcggc cggcaccttc aagggtgcga tcaacatg
//
LOCUS       DASWIY010000241         5856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3462917, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000241 DASWIY010000000
VERSION     DASWIY010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3462917"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VGA59_08855"
     CDS             complement(<1..212)
                     /locus_tag="VGA59_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719826.1"
                     /translation="MADALAPLGEPRLSVITANPAKIRAEQGWPAFSPRQTPARLLAN
                     LLSADALVFTGGTPFFNDRAHMSYYAS"
     gene            complement(446..955)
                     /locus_tag="VGA59_08860"
     CDS             complement(446..955)
                     /locus_tag="VGA59_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HEX9719827.1"
                     /translation="MDESPSAPRAALRPGARVAVVMERETAPNRWEDWRFRVVEVTED
                     EGGFGAAPRVMRDDGKRRWTLHPGFTVELFKDEGEGYYLNLSSGAPVWFVMWRMDGDD
                     RSEAWPEAVSLSYNEAGRWLDAQEKVDNVPLPDDVAAWLQAFTDEHYRPEVKQRKRPA
                     SFLPPDKRQ"
     gene            1174..2160
                     /locus_tag="VGA59_08865"
     CDS             1174..2160
                     /locus_tag="VGA59_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9719828.1"
                     /translation="MSLRLLALAAAFVLAHTAAAAADPIVIKYSHVVADATPKGQAAL
                     KFKELAEKKLPGKVQIQVFPNSQLFGDGKEMEALLLGDVQIIAPSLAKFGKYTPKIQV
                     FDLPFLFDNIAAVDRFQDGPKGQELLNSMTSKGIKGLGYLHNGMKQLSASKPLRTPAD
                     AKGLKFRIQPSDVLEAQFKAVGGNPQKLAFAEVYQALQTGVVDGTENPWSNIYTKKFH
                     EVQKYIMESDHGVLDYMVITNSGWWDKLPADIRKGLNEAMVESIKYGNQVAFNEAATF
                     RQKIIDDKKAQVLPMTKAEIKQWREAMKPVWKKFEADVGKDVIDAALSANAL"
     gene            2282..2809
                     /locus_tag="VGA59_08870"
     CDS             2282..2809
                     /locus_tag="VGA59_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9719829.1"
                     /translation="MKILDHLEELIVTVMLTLMTLLTFSQVVMRYGFNSGFSWALELT
                     TVFFAVMIFVGISYGVRVGAHIGVDALVKKLPPGLKRATSIVVVLLCLLYAGLVTYGS
                     WVYVSKMKTVGVELEDLPIQLWIVRSILPLGYALLGFRFLQVLWALVTGKSDSLHLGD
                     EVAEAMKYKSEETES"
     gene            2806..4086
                     /locus_tag="VGA59_08875"
     CDS             2806..4086
                     /locus_tag="VGA59_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9719830.1"
                     /translation="MNTLVLFTCLFLFMALGMPVAISLGLSSLLTILVFGQDSLASLS
                     IKLYETSEHYTLLSIPFFILAGAMMSTGGVAKRMVRFAIAAVGHLRGGLAIASVLACT
                     LFAAVSGSSPATVVAIGSIVIAGMVKSGYTKEFAAGVICNAGTLGILIPPSIVMVVYA
                     AVTEESVGRMFMAGIIPGLLLSFLLALAVWWRAGKLTLARSERASAREVAVAFKDSFW
                     GLALLAIIMGGIYGGIFTPTEAAAVSAVYAAFVAVVIYRDISIRHLPEVIKDAGKTTV
                     MLMFIIANALLFAHVLTTERIPQTIAEHIVAAGMAPWMFLLVVNIILLVAGNFMEPTG
                     IILILAPIFFPIATQLGIDPIHLGIIMVVNMEIGMITPPVGLNLFVTSGITGMNLVQV
                     TRAALPWLSILLVFLLLVTYVPQISLFLPQLLLD"
     gene            complement(4208..5299)
                     /gene="apbC"
                     /locus_tag="VGA59_08880"
     CDS             complement(4208..5299)
                     /gene="apbC"
                     /locus_tag="VGA59_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEX9719831.1"
                     /translation="MPVTESALLDAIKTVTDPNTGRDFVSTKQLKNLRIEGGDVSFDV
                     ELGYPAKSQVAGLRKSLIAAVRGVPGVENVSANLATKVVAHAVQRGVQLLPKVKNIVA
                     VASGKGGVGKSTTAVNLALALAAEGASVGILDADIYGPSQPMMMGIAGRPESQDGKTM
                     EPMENYGVQVMSIGFLVDADNPMIWRGPMATQALEQLLRQTNWRDLDYLIVDLPPGTG
                     DIQLTLSQRVPLTGAVIVTTPQDIALLDAKKGVKMFEKVGVPILGVVENMAVHVCEKC
                     GHVEHIFGADGGKKMAAEYGIDYLGALPLNLSIRVQADGGRPSVVNEPDGEIAGLYKS
                     VARQTAVKIAQRAKDFSSKFPTIAISKDT"
     gene            5436..>5856
                     /locus_tag="VGA59_08885"
     CDS             5436..>5856
                     /locus_tag="VGA59_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9719832.1"
                     /translation="MPRKLFVTTALPYANAPFHVGHMMEYIQADVWVRFQRMQGNEVH
                     FVCADDAHGAPIMIAAEKAGKSPQQYVAEIAAGRKQFLDGFHIGFDNWHSTDGPENHE
                     LAKDIYRALRREGLIAVRSVEQFYDPVKGMFLPDRYIK"
BASE COUNT          948 a         1946 c         1902 g         1060 t
ORIGIN      
        1 gaggcgtagt agctcatgtg ggcccgatcg ttgaaaaacg gcgtcccccc ggtgaagacc
       61 agggcgtcgg ccgagagcag gttggccagc aagcgggcgg gggtctgcct gggggagaac
      121 gccggccagc cctgctcggc ccggattttg gcggggttgg ccgtgatcac gctcaagcga
      181 ggctcgccca gcggcgccaa ggcgtcggcc atgccctgca gcatcgcgtg gtcgccgatg
      241 ttgcggctgc tgaaccagcc gccggtcagg cagacgtggg ggcggtcgcc ctcctctggc
      301 cgcctctcgc ttggggagag ggcttccctt tccttctccc tctccctctg ggagagggct
      361 ggggtgaggg cgggctgccg acgactgcac ctccgccttc ctcctcgccc accgcgccaa
      421 gaacccttca tcgctcatcg ccacctcact gccgcttgtc cggcggcaaa aacgacgccg
      481 gccgcttgcg ctgcttcacc tcgggccggt agtgctcgtc ggtgaaggcc tgcagccagg
      541 cggccacgtc gtcgggcagc ggcacgttgt ccaccttctc ctgcgcgtcc agccagcggc
      601 cggcttcgtt gtacgacagg ctcaccgcct cgggccaggc ctcggaacgg tcgtcgccat
      661 ccatgcgcca catcacgaac cacaccggcg cgcccgagct gaggttgagg tagtagccct
      721 cgccttcgtc cttgaacagt tctacggtga aacccggatg cagcgtccag cgacgcttgc
      781 cgtcgtcgcg catcacgcgc ggcgcggcgc cgaaaccgcc ctcgtcctcg gtcacttcca
      841 ccacccggaa gcgccagtct tcccagcggt tgggtgcggt ttcgcgctcc atcacgaccg
      901 ccacgcgcgc accggggcgc agcgcggcgc gcggggcgga aggcgattcg tccatcgggt
      961 gttcctggag atcgactagg acaagtctag ggcctgttcg ctccaaatac gtgaccgcgt
     1021 aacccatgaa aggcgccggc gccggcagtt gacgcagcgc aacgcacccg cgcttgacgc
     1081 gcatttcaag tagtttccac aacgccgccg gcggctccct ccaatagatt tgtccacatt
     1141 gttcgttcac accacagcaa gaggagaagc acgatgtcac tgcgtctatt ggcccttgcg
     1201 gccgcattcg ttctcgcgca cacggccgcc gccgcggcgg acccgatcgt catcaagtac
     1261 agccacgtcg tggccgacgc cacgcccaag ggccaggcgg cgctgaagtt caaggaactg
     1321 gccgagaaaa agctgcccgg caaggtgcag attcaggtgt tcccgaacag ccagctgttc
     1381 ggcgacggca aggagatgga agcgctgctg ctcggcgacg tgcaaatcat cgccccgtcg
     1441 ctggccaagt tcggcaagta cacgcccaag atccaggtgt tcgacctgcc cttcctgttc
     1501 gacaacatcg ccgccgtcga ccgtttccag gacggcccca aggggcagga gttgctgaac
     1561 tcgatgacca gcaagggcat caagggcctg ggctacctgc acaacggcat gaagcagctg
     1621 tcggccagca agccgctgcg caccccggcc gacgcgaagg gcctgaagtt ccgcatccag
     1681 cccagcgacg tgctggaggc acagttcaag gccgtcggcg gcaacccgca gaagctggcc
     1741 ttcgccgagg tctaccaggc actgcagacc ggcgtggtcg acggcaccga gaacccctgg
     1801 tccaacatct acaccaagaa gttccacgag gtgcagaagt acatcatgga gtccgaccac
     1861 ggcgtgctcg actacatggt catcaccaac tccggctggt gggacaagct gcccgccgac
     1921 atccgcaagg gcctgaacga ggcgatggtc gagtcgatca agtacggcaa ccaggtcgca
     1981 ttcaacgaag ccgccacctt ccgccagaag atcatcgacg acaagaaggc ccaggtgctg
     2041 cccatgacca aggccgagat caagcaatgg cgcgaggcga tgaagccggt gtggaagaag
     2101 ttcgaagcag acgtcggcaa ggacgtcatc gacgcggcgc tgagcgcgaa cgccctctga
     2161 tcgtcgattt cgtcatgggc cgcattgcgc aggccccctc agggccccgc ctcggggccc
     2221 tgttttttga gttcggccgc ggtagcatgc gaggcggccg gctagggtac ggagggactc
     2281 catgaaaatc ctcgaccacc tcgaggaact gatcgtcacg gtgatgctga cgctgatgac
     2341 gctgctgacg ttctcgcaag tcgtcatgcg ctatggcttc aactcgggct tctcctgggc
     2401 gctcgagctc accacggtgt tcttcgcggt gatgatcttc gtcggcattt cctacggcgt
     2461 gcgcgtgggc gcgcacatcg gcgtggacgc cttggtcaag aagctgccgc ccggcctgaa
     2521 gcgcgccacg tccatcgtcg tcgtgttgct gtgcctgctg tatgccggcc tggtgacgta
     2581 cggctcgtgg gtctacgtga gcaagatgaa gacggtcggc gtcgaactcg aagacctgcc
     2641 gatccagctt tggatcgtgc ggtcgattct cccgctgggc tacgcgctgc tcgggttccg
     2701 cttcctccag gtgctgtggg cgctggtcac ggggaagtcc gacagcctgc acctgggcga
     2761 cgaggtcgcc gaagcaatga aatataagag cgaggagacc gaatcgtgaa cacccttgtc
     2821 ctgttcacct gcctgttcct gttcatggcc ctgggcatgc cggtggccat ttcgctgggc
     2881 ctgtccagcc tgctgaccat cctggtcttc ggacaggact cgctggccag cctgtcgatc
     2941 aagctgtacg aaaccagcga gcactacacg ctgctgtcca tccccttctt catcctggcc
     3001 ggcgcgatga tgagcaccgg cggcgtggcc aagcgcatgg tgcgttttgc catcgccgcg
     3061 gtgggccacc tgcgcggcgg gctggcgatt gccagcgtgc tggcctgcac gctgttcgcg
     3121 gccgtctcgg gctccagccc ggccacggtg gtggccatcg gctccatcgt gatcgccggc
     3181 atggtgaaaa gcggctacac gaaggagttc gccgccggcg tgatctgcaa cgcgggcacg
     3241 ctgggtatcc tgatcccgcc gtccatcgtg atggtggtgt acgccgcggt caccgaggag
     3301 tcggtcggcc gcatgttcat ggccggcatc atcccgggcc tgctgctgtc tttcctgctg
     3361 gcgctggcgg tgtggtggcg cgccggcaag ctgacgctgg cgcgcagcga acgcgccagc
     3421 gcacgcgaag tggcggtggc cttcaaggac tcgttctggg gcctggcgct gctggccatc
     3481 atcatgggcg gcatctacgg cggcatcttc acgcccaccg aggccgccgc ggtgtcggcg
     3541 gtgtacgcgg ccttcgtcgc ggtggtgatc taccgcgaca tcagcattcg tcacttgccg
     3601 gaggtgatca aagacgccgg caagaccacg gtcatgctga tgttcatcat cgccaacgcg
     3661 ctgctgttcg cgcacgtgct gacgaccgaa cgcattccgc agacgattgc cgagcacatc
     3721 gtggccgcgg gcatggcgcc atggatgttc ctgctggtgg tcaacatcat cctgctggtg
     3781 gcgggcaact tcatggagcc caccgggatc atcctgatcc tcgcgccgat cttcttcccg
     3841 attgccacgc agctgggcat cgacccgatc cacctgggca tcatcatggt ggtgaacatg
     3901 gagatcggca tgatcacgcc accggtgggg ctgaacctgt tcgtcaccag cggtatcacc
     3961 ggcatgaacc tggtgcaggt cacgcgcgct gcgctgccct ggctttcgat cctgctggtg
     4021 ttcctgctgc tggtgaccta cgtgccgcag atcagcttgt tcctgccgca gctgctgctg
     4081 gattgaccgg aatggccggg ccgcgctggc agcgcggccc ggcggtgctc aaggaaacgc
     4141 cgggccgctc ccaagtttcc ttgaccccct cggggggcct gacgcgaagc ggcaggtttg
     4201 ggggcgctca ggtgtccttg gagatcgcga tcgtcgggaa cttcgagctg aagtccttgg
     4261 cgcgctgggc gatcttcacc gccgtctggc gcgccacgct cttgtacagg ccggcgatct
     4321 cgccgtcggg ctcgttgacc acgctgggcc ggccgccatc ggcctgcacg cggatgctca
     4381 ggttcagcgg cagcgcgccg aggtagtcga tgccgtattc ggcggccatc ttcttgccgc
     4441 cgtcggcgcc gaagatgtgc tccacgtggc cgcatttttc gcacacgtgc acagccatgt
     4501 tttccaccac gccgaggatg ggcacgccca ccttctcgaa catcttcacg cccttcttgg
     4561 cgtcgagcag cgcgatgtct tgcggggtgg tgacgatcac cgcgccggtc agcggcacgc
     4621 gctggctgag cgtgagctgg atgtcgccgg tgcccggggg caggtcgacg atcaggtaat
     4681 cgaggtcgcg ccagttggtc tggcgcagca gttgttcgag cgcctgggtg gccatggggc
     4741 cgcgccagat catcgggttg tcggcgtcga cgaggaagcc gatggacatg acctgcacgc
     4801 cgtagttttc catcggctcc atggtcttgc cgtcctgcga ctcggggcgg ccggcgatgc
     4861 ccatcatcat cggctggctg gggccgtaga tgtcggcatc gaggatgccc accgacgcgc
     4921 cttcggccgc cagcgccagc gccaggttga ccgcggtggt gctcttgccc acgccgccct
     4981 tgcccgaggc caccgcgacg atgttcttca ccttgggcag caactgcacg ccgcgctgca
     5041 ccgcgtgcgc aacgaccttg gtggcgaggt tggcgctgac gttttccacc cccggcacgc
     5101 cgcgcaccgc cgcgatcagg ctcttgcgca ggccggcgac ctggctcttg gcggggtagc
     5161 ccagctcgac gtcgaaggac acgtcgccgc cttcgatgcg caggttcttg agctgtttgg
     5221 tcgagacaaa atcgcggccg gtgttggggt cggtgacggt cttgatcgcg tcgagcagcg
     5281 cggattcggt gacgggcatg gggcttgaaa aacgatggtg aaagtcttcg cggcagtcta
     5341 ccccgctgca cgtgcccgtg cggtcctgcg tgcggcgggg cctagaatcc gggcttttcc
     5401 ggacacgtcg cggccccgtt cctgccgccg cccccatgcc gcgcaagctc ttcgtcacca
     5461 ccgccctgcc gtacgccaac gcacccttcc acgtcgggca catgatggag tacatccagg
     5521 ccgacgtgtg ggtgcgtttc cagcgcatgc agggcaacga agtgcacttc gtctgcgccg
     5581 acgacgcgca tggcgcgccg atcatgatcg ccgccgaaaa ggcgggcaag tcgccgcagc
     5641 agtacgtggc cgagatcgcc gccgggcgca agcagttcct cgacggcttt cacatcggct
     5701 tcgacaactg gcattccacc gacgggcccg agaaccacga actggccaag gacatctacc
     5761 gcgcgctgcg ccgcgaaggc ctgatcgccg tgcgctcggt cgagcagttt tacgacccgg
     5821 tcaagggcat gttcctgccc gaccgctaca tcaagg
//
LOCUS       DASWIY010000242         9482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2744566, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000242 DASWIY010000000
VERSION     DASWIY010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9482
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2744566"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VGA59_08890"
     CDS             complement(<1..566)
                     /locus_tag="VGA59_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX9719833.1"
                     /translation="MRWEGNRKSGNVEDRRGEGGGRGGFGLGGRSIGIGTVVIALVGG
                     AIFGINPLTILSFLTGGGAPAQVQQQGPAKAPPADDKMAKFVSTVLADTEDVWKQLFT
                     QGGTAYKEPRLVLFRGAVATGCGQGQAVMGPFYCPADQKVYIDLNFYETLKTRMGAPG
                     DFAQAYVIAHEVGHHVQNQLGITGQMARL"
     gene            606..1277
                     /gene="pcp"
                     /locus_tag="VGA59_08895"
     CDS             606..1277
                     /gene="pcp"
                     /locus_tag="VGA59_08895"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEX9719834.1"
                     /translation="MVSASDAAVGRFPTVLVTGFDPFGGASINPSWMAAQALHGRLVA
                     GHRLIGARLPTVFGQSLQSLLELVHRHRPALVVCAGQAAGRAALSLERVAINVNDAHI
                     ADNAGAQPVDTPVVPGGPAAYFTSLPIKAMLVSLLAEGINAEVSQTAGTFVCNHVFYG
                     LMHELATRHELGRTRGGLIHVPWLPAQGQPSMRLDEIVQGLRTAIQCALVTVEDIRKE
                     AGALT"
     gene            1364..2215
                     /locus_tag="VGA59_08900"
     CDS             1364..2215
                     /locus_tag="VGA59_08900"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HEX9719835.1"
                     /translation="MPILNADLHCHSVVSDGTLTPEDLAARAAANGVELWSLTDHDEI
                     GGQERAAAAARAHGVKYLTGTEISVTFADQTVHIVGLGFDANDPAMLRGLAATRGGRG
                     ARAREMAGQLARAGIERTFEGALKYVGNPALISRTHFARYLVETGVCKDTGEVFRRYL
                     TEGNPGYVPHRWASLKAAIQWITSAGGMAVIAHPARYKFTANEEFALFSEFKAHGGQG
                     VEVVTGSHTAAEYVRYAETAREFGLAASRGSDFHSPDESRTDLGQLPFLPGELTPVWE
                     VLADRIQ"
     gene            2269..3126
                     /locus_tag="VGA59_08905"
     CDS             2269..3126
                     /locus_tag="VGA59_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9719836.1"
                     /translation="MVLGACACFAALDTTTKYVSAFVPLMMALWFRYAFQAIATTVSV
                     LPVRGTSVFRTRHPEFQCLRGVLLLASSLLAFASLKHMPVGEFTAIVMITPLAITVLA
                     ATVLKEKVSPMRWILVAGGFAGTLVIIRPGGQAFNWASLLPLGLVVTNAWFQVLTSKL
                     ARTEDPVTMHLYTGWVGTLLASIALPWVWTALPAWWLWLALCFMGLMATVGHFFLILA
                     YQRAPAATLTPYLYMQIAFAMMAGWLVFSHVPDEWSMLGIAMIAVCGAGGAWLTVRES
                     RLALEPIEV"
     gene            3137..3772
                     /locus_tag="VGA59_08910"
     CDS             3137..3772
                     /locus_tag="VGA59_08910"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX9719837.1"
                     /translation="MAQYFDVHPDNPQVRLLRQAVALLEGGGIVAVPTDSSYAFACHL
                     DDKSAVDRLRRIRGMDHRHHLTLLCRDLSELANYARVDNRQYRLLKSATPGAYTFILD
                     ATREVPRRVSHPQRKTVGLRVPDHRVLQELLAIHGAPLLATTLIPPRETEPLNDAQQI
                     RDRFEHQIAGVIDAGACPSEPTSVIDLTPMGNGGEAVVVRRGRGDVAALGL"
     gene            4150..4824
                     /locus_tag="VGA59_08915"
     CDS             4150..4824
                     /locus_tag="VGA59_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9719838.1"
                     /translation="MNISNLIQTVLIYALPVLFAITIHEAAHGYVARALGDNTAYAMG
                     RVTLNPLKHIDPVGTILMPLMLYFATGGTFLFGYAKPVPVNFGQLRRPKRDMIWVALA
                     GPASNLIQALLWAVLFAVLRFSGVEERFFLIMALAGVLVNLVMWAFNLFPLPPLDGGR
                     ILVGLLPWRAANALSRVEPWGFFIVLGLVLLGVVGAYWLLPLIKLGYLALDLLNLPTP
                     FPPRLR"
     gene            4821..6110
                     /locus_tag="VGA59_08920"
     CDS             4821..6110
                     /locus_tag="VGA59_08920"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX9719839.1"
                     /translation="MSPVRFLTGITTTGTPHLGNYVGAIRPAVEASRRAGTENFYFLA
                     DYHALIKCDEPARIQRSTLEIAACWLAAGLDPERVFFYRQSDLPEVTELTWLLTCVTG
                     KGLLNRAHAYKAAVDKNLAAGTDPDAEVTAGLFMYPVLMAADILLFKAHKIPVGRDQV
                     QHIEMARDMAQSFNHLYGEHFVAPEAAIDESVAMLPGLDGRKMSKSYDNIIPLFAPPA
                     QMRKQIAGIVTDSRAPGEPKLVEGSALFQIYQAFASPQETQALRQAYADGIAWGDAKQ
                     LLFERIDREIAPMRERHDALVNNPAELEKILRAGAEKTRGLARPFMAELRDAVGLRNL
                     AATAQGAAKTKTSRYALPSFKQYREADGRHYFKLVDGGGRLLARSAGFDSPQQAGRTI
                     AQLKKDGPAGLEADLELAGGVDAAEVAAALAQLAQAQ"
     gene            6204..6797
                     /locus_tag="VGA59_08925"
     CDS             6204..6797
                     /locus_tag="VGA59_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9719840.1"
                     /translation="MSVYVIDDHPLMREAVVMLMRRLRPGATVVELDRIGGVEAAVKQ
                     HGPPDLICLDLKLPDTTGTSGIHEMKNRFPETPLAVLSASPAADAEEPCIEAGADIYI
                     EKSAGAQEIGNAMRALLTADGGFEELTPTDNKLSKRQKQLIVMLDRGLSNREIADELG
                     ISEHTVKVHLWRLFRRLGVKSRTQTIHYARTHGMLTS"
     gene            complement(6720..8192)
                     /locus_tag="VGA59_08930"
     CDS             complement(6720..8192)
                     /locus_tag="VGA59_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9719841.1"
                     /translation="MKLDVPPAIATPAEAQWVEGELVRSLMKIARNTQLIGLLLIPVF
                     VGVLWGNAMLGALLVWALAALAIAGVRFAIIHKYVREVTSGDAARPLKFFRRYRALWP
                     ASAFVWGASTLLYFDRSSLADQFICWLILAGLAMFSINSLSSHLATLRAYLDTLAITA
                     LAVILWRVGVELNFQGPTYHYWIISLLVIFWQVLRQAGLRLHETHRKNFELQYRNNQL
                     IESLTRQTQAALDAVEIKNRFLASAAHDIRQPVHALSLYADWLGSEPELVHEIAPKIV
                     ESTKAVNALFDSLFDLVRLDTGKIKINIEPVDLGQLLHDLELQYRPLAQAKGLRFLVH
                     VVPGTVLSDAILLRRIVGNLVSNAVKYTSRGGVLLASRATPKGWRIDIWDTGVGIAPV
                     HQREIFREFYKVPSHAGTEEGFGLGLYIVARLSTILGHPLSLASWPGKGTVFRLMLQP
                     TDAQEAATRAADFGGLTGQHAVSASVVDSLGAALHPQPAE"
     gene            complement(8267..8647)
                     /locus_tag="VGA59_08935"
     CDS             complement(8267..8647)
                     /locus_tag="VGA59_08935"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX9719842.1"
                     /translation="MDIGSARSAPAGPGASLSAATSFFARTVVGADGEVLGSISDLML
                     DLDRGRVAYAVVATGGIVGIGERLFAVPWSALRPLGEQFLLECKRAVLDSAPAFDRDH
                     WPLTPAHRWHERVHAHYHSRPYWE"
     gene            8788..>9482
                     /locus_tag="VGA59_08940"
     CDS             8788..>9482
                     /locus_tag="VGA59_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719843.1"
                     /translation="MDRFGRSRPASLRTYLFGVILLAIVPIGAFMSARIFMDVQGEQA
                     LTEDELARSAAALSQAMERELVSSIDALTVLSQSGLVQQGRVADLGQLLRWRPRRDWD
                     SLFVLDPGGEVLLDTASPAGSATQRGQSLRELHRRVAGELRPAVSGVADARAPARRMI
                     AVALPVVQDGALRYVLGARIGGDVWQQLASTASMPAGAHAGMFDARGVLIDYWPGPAH
                     AGMVLPPETAAAMA"
BASE COUNT         1597 a         3224 c         3103 g         1558 t
ORIGIN      
        1 agccgggcca tctggccggt gatgcccaac tgattttgca catggtggcc aacctcgtgg
       61 gcgatcacgt aggcctgcgc gaagtcgccg ggggcgccca tgcgggtctt gagggtttcg
      121 tagaagttca ggtcgatgta aaccttctgg tcggctgggc agtagaacgg gcccatcacg
      181 gcctggccct ggccgcagcc ggtggcgacg gcgccgcgaa acagcaccag ccgcggctcc
      241 ttgtaggcgg tgccgccctg agtgaacaac tgtttccaga cgtcttcggt atcggccagc
      301 accgtggaaa cgaacttggc catcttgtcg tcggccggcg gagccttggc cgggccttgc
      361 tgctgcacct gggcaggggc cccgcccccg gtgagaaagc tcaggatcgt gagcggattg
      421 atgccgaaga tcgcgccgcc gaccagggcg atgacaaccg tgccgatgcc gatgctgcgc
      481 ccgcccaaac cgaagccacc gcggccgccg ccttcgcccc ggcggtcctc gacattgccg
      541 gatttacgat taccttccca acgcatgctg ttctcccggt gaccgcgctg cggtacagtg
      601 aaaccatggt atccgcatca gacgcggccg tgggccgctt tcccaccgtg ttggtaaccg
      661 ggttcgatcc attcggcggc gcctcgatca accccagctg gatggcggct caagcactgc
      721 acgggcggct ggtggccggg catcggctca tcggggcgcg gctacccacg gtgttcggcc
      781 aatcgctgca gagcctgctc gaattggttc atcgccaccg gccggcgctg gtggtgtgcg
      841 ccggccaggc tgccggccgg gcggcactgt cgctggagcg cgtcgcgatc aatgtcaacg
      901 acgcgcatat tgccgacaac gcgggcgccc agcccgtgga tacgccggtc gtgccgggcg
      961 gccccgcggc ctatttcacc agcctgccga tcaaggccat gctggtctcg ctgctggccg
     1021 aaggcatcaa cgccgaggtg tcgcaaaccg ccggcacgtt cgtctgcaac cacgttttct
     1081 acggcctgat gcacgagctg gcgacgcggc acgagctggg tcgcacgcgc ggcgggctca
     1141 tccacgtgcc gtggctgccg gcgcagggcc agccctccat gcggctggac gagatcgtgc
     1201 agggactgcg cacggcaatc caatgcgcgc tggtcacggt ggaggacatc cgcaaggaag
     1261 cgggagcgct gacttgacgc cgcccgcggc cggcggcgcc gccggcgaat gggtttgcgc
     1321 ggcacggcag acgtgaaaac ccgcgcacgc ctaaaatcaa aaagtgccca ttctcaatgc
     1381 cgaccttcac tgccactccg tcgtatccga tggcacgctc acaccggaag atttggctgc
     1441 ccgtgccgcc gccaacggcg tggaactgtg gtccttgacc gaccacgatg aaatcggcgg
     1501 ccaggagcga gcggccgcgg cagccagggc gcatggcgtc aagtacctca cggggaccga
     1561 aatctcggtg acgttcgccg accagaccgt tcacatcgtc ggcctgggct tcgacgcgaa
     1621 cgaccccgca atgctgcgcg gcctcgcggc aacacgcggc gggcgcggcg cccgggcccg
     1681 agagatggcc gggcagctgg cccgggcggg catcgagcgg accttcgaag gtgcgctcaa
     1741 gtacgtcggc aatcccgcac tgatctcgcg cactcacttc gcccgctacc tggtggagac
     1801 gggggtctgc aaggacacgg gcgaggtgtt ccgcaggtac ctgaccgaag gcaatccggg
     1861 ctacgtgccg caccgctggg cgtcgctgaa ggcagcgatc caatggatca ccagtgccgg
     1921 cggcatggcc gtgatcgccc accctgcgcg ctacaagttc acggcgaatg aagagttcgc
     1981 gctgttcagc gaattcaagg cccatggcgg ccagggtgtg gaagtcgtga ccggcagcca
     2041 tacggcggcc gagtacgtga ggtacgccga aaccgcgcgc gagttcggcc tggcggcttc
     2101 gcgcggcagc gatttccaca gcccggacga aagccgcacc gacctgggcc agctgccctt
     2161 cctgcccggc gagctcacgc cggtctggga agtgctggcc gaccgcatcc agtgaagccg
     2221 ccgcgcggga cttcggcgga cgcccagggc acgctggccg gcatcgcgat ggtgttgggg
     2281 gcctgcgcct gcttcgcggc gctggacacg acgaccaaat acgtcagcgc tttcgtgccg
     2341 ctgatgatgg cgctgtggtt tcgctacgcg ttccaggcga tcgccaccac ggtttccgtg
     2401 ctgccggtgc gcgggacgtc ggtgtttcgc acccggcacc ccgaattcca gtgcctgcgc
     2461 ggcgtgctgc tgctcgccag cagcctgctc gccttcgcca gcctgaagca catgccggtc
     2521 ggcgagttca cggccatcgt gatgatcacg ccgctggcga tcacggtgct ggccgccacc
     2581 gtcctgaagg aaaaggtctc gcccatgcgc tggattctgg tcgccggcgg ctttgccggc
     2641 accctggtca tcatccggcc cggcgggcag gccttcaact gggccagcct gctgccgctg
     2701 gggctggtcg tcaccaacgc ctggttccag gtcttgacca gcaaactcgc gcgcaccgag
     2761 gatccggtca cgatgcacct gtacaccggc tgggtcggca cgctgctcgc gtcgatcgcg
     2821 ctgccgtggg tatggaccgc cctgcccgcc tggtggctct ggctggcgct gtgcttcatg
     2881 ggcctgatgg ccaccgtggg ccatttcttc ctgatcctgg cctaccagcg cgcgcccgcg
     2941 gcgacgctca cgccctacct ctacatgcag atcgcctttg ccatgatggc cggctggctg
     3001 gtgttctcgc acgttccgga cgagtggtcg atgctgggca tcgcgatgat cgcggtctgc
     3061 ggcgccggcg gggcctggct caccgtgcgc gagagccggc tcgcactcga gccgatcgaa
     3121 gtctgaaggc attgcgatgg cccagtactt cgacgtgcac cccgacaacc cgcaggtccg
     3181 gctgctcaga caggctgtcg cactgctcga aggcggtggc atcgttgccg ttcccaccga
     3241 ttcgagttac gcgttcgcct gccacctcga cgacaaatcg gcggtcgacc ggctgcgccg
     3301 gatccgcggc atggaccaca ggcaccatct cacgctgcta tgccgcgact tgagcgagct
     3361 ggccaactac gcgcgcgtgg acaaccggca gtaccggctg ttgaaatccg ccacgccggg
     3421 cgcctacacc ttcatcctgg atgccacgcg cgaagtgcca cggcgcgtca gccatccgca
     3481 gcgcaagacc gtcggtttgc gcgttcccga ccatcgcgtg ctgcaggagc tgctggccat
     3541 ccatggcgcg ccgctgctgg cgaccacgct gattcccccg cgcgagaccg agcccctgaa
     3601 cgacgcgcaa cagatccgcg accgtttcga acaccagatc gccggtgtga tcgacgccgg
     3661 ggcctgcccg tccgagccga ccagcgtgat cgacctcacg cccatgggca acggcggcga
     3721 agcggtggtc gtgcgccggg ggcgcggcga cgtggcggcg ctgggtttat gaagagcaat
     3781 gggggcgccg acaatggatt gcggctcagg cccgcaatga cagttggatt tgtcatcccg
     3841 ggcttgaccc gggatccatg acttcctgga ttgcgggtca cgcccgcaat gacagttgga
     3901 tttgtcatcc cgggcttgac ccgggatcca tggcttcctg gattgcgtgt caggcccgca
     3961 atgacagttg gatttgtcat cccgggcttg acccgggatc catggcttcc tggattgcgg
     4021 gtcaggcccg caatgacaag attcagactc cggatcaatt ccggggcggg cccgatgccg
     4081 gatcccaatg atccgggatg acagccaggg gacagaaggg aacaggcgcc gtctgagaca
     4141 atccccctcg tgaatatctc aaacctgatt caaaccgtgc tgatctacgc gctgccggtg
     4201 ctgttcgcga tcaccatcca tgaggcggca catggctacg tggcgcgggc cctgggcgac
     4261 aatacggcgt acgcgatggg ccgggtgacg ctgaacccgc tcaagcacat cgatcccgtc
     4321 ggcacgatcc tgatgccgct gatgctttac ttcgcgaccg gtggaacatt cctgttcggc
     4381 tatgccaagc cggttccggt gaacttcggc cagctgcgcc gccccaagcg cgacatgatc
     4441 tgggtcgcgc tggcgggccc ggcctccaac ctgatccagg ccttgctgtg ggccgtgctg
     4501 ttcgccgtcc tgcgcttttc cggcgtggag gaaagatttt tcctgatcat ggccctggcc
     4561 ggcgtgctgg tgaacctggt gatgtgggcg ttcaatctct tcccactgcc gccgctggac
     4621 gggggccgaa tcctggtggg gctgctgccc tggcgcgcgg cgaacgccct cagccgtgtc
     4681 gagccctggg gctttttcat cgtgctggga ctggtgctgc tgggcgtcgt gggcgcctac
     4741 tggctgttgc ctctgataaa gctcggctat ctcgctctgg acctgcttaa cctgcccacc
     4801 ccgtttccgc cccgactccg atgagccccg tccgattcct caccggcatc accaccaccg
     4861 gcacgcccca cctcggcaac tacgtcggcg cgattcgccc ggcggtcgag gccagccgcc
     4921 gcgccggcac cgagaacttc tatttcctgg ccgactacca cgcgctgatc aagtgcgacg
     4981 agcccgcccg catccagcgc tcgacgctgg agatcgcggc ctgctggctg gcggccggac
     5041 tcgatcccga gcgtgtgttc ttctaccgac agtccgacct ccccgaagtc accgagctga
     5101 cctggctgct gacctgcgtc accggcaagg ggctgctcaa tcgcgcccac gcctacaagg
     5161 cggcggtcga caagaacctt gccgccggca ccgaccccga cgccgaagtg acggcgggcc
     5221 tgttcatgta cccggtgctg atggcggccg acatcctgct gttcaaggcg cacaagatcc
     5281 cggtgggccg cgaccaggtg cagcacatcg agatggcgcg cgacatggcg cagagcttca
     5341 accatcttta tggcgagcat ttcgtggcgc cggaagcggc gatcgacgaa tcggtggcca
     5401 tgctgcccgg cctggacggg cgcaagatga gcaagagcta cgacaacatc atcccgctgt
     5461 tcgcgccgcc cgcgcaaatg cgcaagcaga tcgcgggcat cgtgaccgac tcgcgcgccc
     5521 ccggcgagcc gaagctcgtc gaaggctcgg ccctgttcca gatctaccag gcgttcgcct
     5581 cgccgcaaga gacgcaggcg ctgcgccagg cctatgccga cggcattgcc tggggcgatg
     5641 ccaagcagct gctgttcgag cggatcgacc gcgagatcgc accgatgcgc gaacgccacg
     5701 atgcgctcgt gaacaacccg gcggaactcg aaaagatcct gcgtgccggc gccgagaaga
     5761 cgcgcggcct ggcccggcct ttcatggccg aactgcgcga cgccgtcggc ttgcgcaatc
     5821 tcgccgccac cgcgcaaggc gccgcgaaaa cgaagacctc caggtacgcg cttccgtcgt
     5881 tcaagcagta ccgggaagcc gacggccgcc actacttcaa gctggtggac ggcggcggcc
     5941 ggctgctggc ccgcagcgcc ggcttcgact cgccccagca ggcgggccgc accattgcgc
     6001 agctcaaaaa ggacggcccg gccgggctcg aagccgacct ggaattggcc gggggcgtgg
     6061 acgcagccga agtcgccgcg gcgctcgcgc aactggccca ggcccaatga gtgttggcat
     6121 gctaaaacac tctgttcaag ccgacccaat cggagttatg cttacttctt atgatgaccg
     6181 ctgaaatcaa gaattcgaaa accatgagcg tctacgtcat cgacgatcac ccgctgatgc
     6241 gggaggccgt cgtgatgctg atgcggcgcc tgcgccccgg cgcaaccgtg gtcgaactgg
     6301 accgcatcgg cggtgtcgag gccgccgtca agcagcacgg cccgcccgac ctgatctgcc
     6361 tggacctgaa gctgcccgat accaccggca cctcgggcat ccacgagatg aagaaccgct
     6421 ttcccgaaac gccgctggcg gtgctgtccg cctcgccggc ggccgatgcc gaggaaccat
     6481 gcatcgaggc gggcgccgat atctacatcg aaaaatccgc gggtgcccag gaaatcggca
     6541 atgcgatgcg cgcgctgctc acggccgacg gcggcttcga ggaactcacg cccaccgaca
     6601 acaagctgtc caagcgccag aaacagctga tcgtcatgct cgaccggggc ctgtccaacc
     6661 gcgaaatcgc ggacgagctc ggcatcagcg aacacacggt gaaggtccac ctgtggcggc
     6721 tattccgccg gctgggggtg aagagccgca cccagactat ccactacgct cgcactcacg
     6781 gcatgctgac cagttaggcc gccgaagtcg gcggcccggg tggcggcctc ctgggcgtcc
     6841 gtgggctgga gcatcaggcg aaacaccgtt cccttgcctg gccaggaggc caggctgagc
     6901 ggatggccca ggatggtgga caggcgagcc acgatgtaaa gccccaggcc gaaaccttcc
     6961 tcggtgcccg catggctggg caccttgtaa aactcccgga aaatctcgcg ctggtgcacc
     7021 ggcgcgatgc ccacgccggt gtcccagatg tcgatgcgcc agcctttggg cgtggcgcgc
     7081 gaggccaaca gcacgccgcc acgggaggtg tacttcaccg cgttggatac caggttgccg
     7141 acgatgcgcc gcagcaggat ggcatccgag agcacggtgc ccggcacgac atgcaccagg
     7201 aaccgcagcc ccttggcctg cgcgagcggc cggtactgca gctccaggtc gtgcagcagc
     7261 tggcccaggt cgacgggctc gatattgatc ttgatcttgc ccgtgtccag ccgcaccagg
     7321 tcgaacagcg aatcgaacag cgcgttcacg gccttggtgg attcgacgat cttgggcgcg
     7381 atctcgtgca ccagttccgg ctcgctgccc agccagtcgg cgtacaggct caaggcgtgc
     7441 accggctggc ggatgtcgtg ggccgcgctc gccaggaagc ggttcttgat ttccaccgcg
     7501 tccagcgcgg cctgcgtctg ccgcgtgagc gactcgatca gctggttgtt gcggtattgc
     7561 agctcgaagt tcttgcggtg cgtctcgtgc agccgcagcc cggcctggcg caagacctgc
     7621 cagaagatca ccagcagcga gatgatccag tagtggtagg tcgggccctg gaagttcaat
     7681 tccacgccga cgcgccacag tatcaccgcc agcgccgtga tcgccagcgt atccaggtaa
     7741 gcgcgcaacg tcgccagatg gctggacagg ctgttgatcg agaacatcgc cagccccgcg
     7801 aggatcagcc agcagatgaa ctggtccgcc agcgacgagc ggtcgaaata cagcagggtc
     7861 gaggcacccc agacgaaggc gctggccggc cacagtgcgc ggtatcggcg gaagaacttc
     7921 agaggccgcg ccgcgtcgcc gctggtcact tcacgcacgt acttgtggat gatcgcaaaa
     7981 cgtacgccgg cgatcgccag cgcggccagg gcccagacca gcagcgcgcc gagcatcgcg
     8041 ttgccccaca gcacgccgac aaaaacggga atcagcagca acccgataag ctgggtattg
     8101 cgcgcgatct tcatcaggct gcgcaccagt tcgccctcca cccactgggc ctcggcgggc
     8161 gtcgcgatcg ccggcggaac gtcgagcttc atggcccgag tctagaacag aaaaaagacc
     8221 cggcaatcga gtggccgcac gtaggaattg cggcaagagc ttccgctcat tcccagtagg
     8281 gacgggaatg gtagtgggcg tgcacgcgct cgtgccagcg atgggccggg gtcagcggcc
     8341 aatggtcgcg gtcgaaagcc ggcgcgctat cgagcaccgc gcgcttgcac tccagcagga
     8401 attgctcgcc cagcggtcgc agcgcgctcc agggcacggc gaacagccgc tcgccaatcc
     8461 ccacgatgcc gccggtggcc accacagcat aggcgaccct gcctcgatcg aggtcgagca
     8521 tcaggtcgct gatgcttccc agtacctcac cgtccgcgcc gacaaccgtc cgggcgaaga
     8581 aagacgtcgc ggcgctcagg ctggcgcccg ggcctgcggg agcggagcgc gcagaaccga
     8641 tatccataat ccgattctcc tggttcacgc ggcaaccacg gcaaggatcg tgccgttacc
     8701 tccggtaacc cgcgcaccat tttcgccggc gttaaggtta ggctcagttc ccgccccaac
     8761 tcgacaatgc gcctgcgcca acaactcatg gaccgatttg gacggtcgcg accggcttcg
     8821 ctgcgcactt acctgttcgg ggtcatcctg ctggccatcg tgcccatcgg cgccttcatg
     8881 agcgcgcgca tttttatgga tgtccagggt gagcaggcgc taaccgaaga cgagctcgcc
     8941 cgctccgctg cggcgctatc gcaggccatg gaacgtgaac tggtctcctc tatcgatgcg
     9001 ctgacggtgc tgtcgcagtc gggcctcgtc cagcagggcc gggtggcgga cttgggacaa
     9061 ctgctgcgct ggcggccgcg ccgcgattgg gacagcttgt tcgtgctgga ccccggcggt
     9121 gaggtgctgc tcgacacggc atcccccgcg ggcagtgcca cgcaacgggg ccagtcgctg
     9181 cgcgagctcc accgccgagt ggcgggcgag cttcggccgg ccgtatccgg cgtggccgat
     9241 gcccgggctc ccgcccggcg catgatcgcc gtggccttgc cggtggtcca ggacggcgcg
     9301 ctgcgctacg tgctcggcgc gcgcatcggc ggcgacgtgt ggcaacagct ggccagcacg
     9361 gccagcatgc ccgcgggcgc gcacgccggc atgttcgatg caaggggcgt gctgatcgac
     9421 tattggcccg gtcccgccca cgcgggcatg gtcttgccgc ccgagactgc cgcggccatg
     9481 gc
//
LOCUS       DASWIY010000243         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_148696, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000243 DASWIY010000000
VERSION     DASWIY010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148696"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VGA59_08945"
     CDS             complement(<1..331)
                     /locus_tag="VGA59_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9719844.1"
                     /translation="MGIDNQPAPSSPPPTRGWSWVPIRSLGQRHRPRIALHLNALDEH
                     DRYLRFGYPATDEQVAHYVDTIDFERDEVFGIFNRRLELIAMAHLANSAAAREAEAPV
                     EAEFGVSV"
     gene            642..1070
                     /gene="rplM"
                     /locus_tag="VGA59_08950"
     CDS             642..1070
                     /gene="rplM"
                     /locus_tag="VGA59_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX9719845.1"
                     /translation="MKTFSAKPAEVTHEWFVIDATDKVLGRVASEVALRLRGKHKAIY
                     TPHVDTGDFIVVVNADKIRVTGNKANDKVYYRHSGYPGGIYGTKFKDMQAKHPGRALE
                     KAVKGMLPKGPLGYAMVKKLKVYAGATHPHTAQQPKVLDI"
     gene            1080..1472
                     /gene="rpsI"
                     /locus_tag="VGA59_08955"
     CDS             1080..1472
                     /gene="rpsI"
                     /locus_tag="VGA59_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX9719846.1"
                     /translation="MIGNWNYGTGRRKSSVARVFMKKGTGQILVNGKPVEQYFGRQTS
                     IMVVKQPLVLTANDAAFDVKVNVHGGGESGQAGAVRHGITRALIDYDAALKPELSRAG
                     FVTRDAREVERKKVGLHGARRRKQFSKR"
     gene            1570..2262
                     /locus_tag="VGA59_08960"
     CDS             1570..2262
                     /locus_tag="VGA59_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEX9719847.1"
                     /translation="MRRRLSVSAPRMIVRSHLPWPLRWALVALVLGFSAAIAMWAFEF
                     GKDIAGLDRNAQAQIARLREELAQLRSERERNLSIANTADSLLRTERAAQERLAAQLK
                     QIEAENLALKADLGFFERLLPAGGGGVSIRGLQAEIKAPGQLRFQLLVLQAGKTLPDF
                     QGQYEITLVGTLDGKPWTFQPPGGARPLHLKQYLRVEGMIDHTPRAVVKTVQVRVMDA
                     SGGVKATQAAKV"
     gene            2297..2692
                     /locus_tag="VGA59_08965"
     CDS             2297..2692
                     /locus_tag="VGA59_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX9719848.1"
                     /translation="MFNKQKQPPIRTLIGEGTAIQGELRFTEGLRIDGEVQGDVLAVG
                     EGHSILVISEKARIVGKVKAAHVIINGHVSGPVECHELLELQPKANIVGNVRYEVLEM
                     HQGATIDGELHPLKVDDKPALKLAASNEG"
     gene            2728..3108
                     /gene="erpA"
                     /locus_tag="VGA59_08970"
     CDS             2728..3108
                     /gene="erpA"
                     /locus_tag="VGA59_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX9719849.1"
                     /translation="MTAVADNFNSSPQADEPPVPLVFTDSAASKVKELVDEEGNPDLK
                     LRVFVQGGGCSGFQYGFTFDEVVNDDDTQMNKNGVTLLIDAMSLQYLAGAEIDYKDDL
                     QGAQFVIKNPNATTTCGCGSSFSV"
     gene            complement(3170..>3263)
                     /locus_tag="VGA59_08975"
     CDS             complement(3170..>3263)
                     /locus_tag="VGA59_08975"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX9719850.1"
                     /translation="KAFVERQPGNLSSVTGAAGPRVLGALYPSQ"
BASE COUNT          680 a         1030 c         1042 g          511 t
ORIGIN      
        1 gcaccgacac gccgaactcc gcctcgacag gcgcctcggc ctcgcgcgcc gccgccgagt
       61 tggccaggtg cgccatggcg atcaactcca ggcggcggtt gaagatgccg aacacctcgt
      121 cacgctcgaa atcaatcgtg tccacatagt gcgcaacctg ctcgtcggtc gctgggtagc
      181 cgaagcgcag atagcggtcg tgctcatcca gcgcgttcag gtgcagcgca atgcgcgggc
      241 ggtggcgttg gcccaaggag cggatcggca cccaagacca gccgcgggtt ggcggcggtg
      301 atgagggggc cggctggttg tcgataccca tgacaaggtt ctcctgaatc tagggttaac
      361 ccgaagtcta ggcgatgtgc ctaggcaagt acccccagga cgccttacac gatagttgcc
      421 tcggaggtgg tcgaggccgg cgtgcgcaac cgctctgata gaatccgcag ctttcccgaa
      481 atcagccccg gggaattcca cgcgacgctg caagcttacg ccccaagccc gcgtgtcctg
      541 cctaagaggc cgccatccaa agaagcggca ccgaacgcag gaacggactc gcgacgaaag
      601 cgcgggccca cagcggctgg tcaaaccaac tcaggtaaac catgaaaacc ttcagcgcca
      661 aaccggccga agtgacgcat gagtggtttg tgattgacgc gaccgacaag gttctcggac
      721 gggtggccag tgaagtcgcc ctccgcctgc gcggcaaaca caaagccatc tacacgcctc
      781 acgtcgatac cggagacttc atcgtcgtcg tcaacgccga caagatccgc gtcaccggca
      841 acaaagccaa cgacaaggtg tactaccgcc actcgggcta ccccggcggt atctacggca
      901 ccaagttcaa ggacatgcag gccaagcacc ccggccgtgc gctggagaaa gccgtcaagg
      961 gcatgctccc caaggggcca cttggctacg cgatggtgaa gaagctgaag gtgtatgccg
     1021 gtgccacgca tccgcacacc gcccagcagc ccaaagtgct ggacatctaa ggagcggtga
     1081 tgatcggaaa ttggaactac ggaaccggcc gccgcaagtc ttcggtggcc cgggtgttca
     1141 tgaaaaaggg cacgggccag attctcgtga acggcaagcc cgtcgagcag tacttcggcc
     1201 gccagacgtc catcatggtc gtcaagcagc cgctggtgct caccgccaac gacgcggcct
     1261 tcgacgtgaa ggtcaacgtg cacggcggtg gcgaatcggg gcaggctggc gcagtgcgcc
     1321 acggcatcac gcgtgcgctg atcgactacg acgcggcact caagcctgaa ctcagccggg
     1381 caggcttcgt gacgcgcgac gcgcgcgagg tcgaacgcaa gaaggttggc ctgcacggcg
     1441 cgcgccgccg caagcagttc agcaagcgct gaggcccacc gctcatcgag caacgaaacg
     1501 gggccgcttg tttgcggcct ctttcgtttc cagtccaact ccgccggctc gaacatgcgc
     1561 tggaaattga tgcgtcgaag gctctcggtc agcgcgccgc gcatgatcgt tcgcagccac
     1621 ctgccctggc cgctgcgctg ggcgctggtg gcattggtgc tcgggttttc ggcggccatc
     1681 gccatgtggg cgttcgagtt cggcaaggac atcgcgggac tggaccgcaa tgcccaagcg
     1741 cagatagcgc gattgcgtga agaactcgct cagttgcgca gcgaacgcga gcgcaacctg
     1801 tcgatcgcga acaccgccga cagcctgttg cgcaccgagc gggcggcgca ggagcggctg
     1861 gccgcgcaac tcaagcaaat cgaggccgaa aaccttgcac tcaaggcgga cctggggttt
     1921 ttcgagcgcc tgctgcctgc gggcgggggt ggcgtcagca ttcgggggct gcaggctgag
     1981 atcaaggcgc cgggccaact tcgatttcag ctgctggtcc tgcaagccgg caagaccctc
     2041 cccgactttc agggccagta cgagatcacg cttgtcggca ccctcgacgg caagccctgg
     2101 accttccagc cgccgggcgg cgcgaggccg ctgcatttga aacagtattt gcgggttgaa
     2161 ggaatgatcg accacacgcc gcgggccgtg gtaaaaaccg tccaagtcag ggtgatggac
     2221 gcgagcggcg gggtcaaggc cacccaagcc gccaaagtct gagggccgcg atgagcggcc
     2281 tgcgttggag ccagccatgt tcaacaagca gaagcagccg ccgatcagaa ccctgatcgg
     2341 cgagggcacc gccattcagg gcgaactacg tttcaccgag ggcttgcgca tcgacggcga
     2401 ggttcaggga gacgtgctcg cagtgggcga agggcacagc atcctggtca tcagcgaaaa
     2461 agcccgcatc gttggcaagg tcaaggcagc gcacgtgatc atcaacggcc acgtcagtgg
     2521 tccggtggaa tgccacgaac tgctcgaact tcagcccaag gcgaacatcg tgggcaacgt
     2581 gcgctacgag gtgctcgaaa tgcaccaggg tgccaccatc gatggagaac tgcacccgct
     2641 gaaagtggat gacaaacccg cactcaagct ggcggcatcg aacgagggat aattcccccc
     2701 tgtgacgccc cgccccaagg atttgccatg accgccgtag ccgacaactt caactccagc
     2761 cctcaggctg atgagccgcc cgttcccctg gtgttcaccg acagcgccgc gtccaaggtc
     2821 aaggaactgg tggacgagga aggcaacccc gacctgaagc tgcgcgtctt cgtgcagggc
     2881 ggcggctgct ccggcttcca gtacggcttc acgttcgacg aagtcgtcaa cgatgacgac
     2941 acgcagatga acaaaaacgg cgtcaccttg ctgatcgatg cgatgagcct gcagtacctg
     3001 gccggcgctg agatcgacta caaggacgac ctgcaggggg cgcagttcgt gatcaagaac
     3061 ccgaacgcga cgaccacttg cggctgtggg tcgagctttt cggtctgatc cccatggcac
     3121 gcaacaaagg ccgccctcgg gtggcctttg tgattgaggg ggcagccggt cactgcgagg
     3181 gatacaacgc cccgaggacg cgtgggccgg cggcaccggt cacgctgctc aggttgccgg
     3241 gctgcctttc gacaaaggcc ttg
//
LOCUS       DASWIY010000244         3975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_535696, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000244 DASWIY010000000
VERSION     DASWIY010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535696"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..601
                     /locus_tag="VGA59_08980"
     CDS             299..601
                     /locus_tag="VGA59_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719851.1"
                     /translation="MKTVMVRYKVKPESAAENEGLIKQVFVQLARDKPERMRYQVFKQ
                     ADGVSFVHVSAFDNPDGNPLTQLEAFKAFTAGIKDRCAEPPVTVELQQVGRYDHLA"
     gene            672..929
                     /locus_tag="VGA59_08985"
     CDS             672..929
                     /locus_tag="VGA59_08985"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9719852.1"
                     /translation="MPTATMTSKGQITVPQAVRSALDLHAGAKVDFVPVAGGFKVVPL
                     RAGSSALKGRFAGRVAKPVSVEQMDEAIAAEAARRHGSGKR"
     gene            933..1340
                     /locus_tag="VGA59_08990"
     CDS             933..1340
                     /locus_tag="VGA59_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9719853.1"
                     /translation="MIGLDTNVLVRYLAQDEPKQAALATRLIEERLSAATPGFISLVV
                     LAELCWVLKSLYGASQSELAATAEDLLSAAQFHLERREVIQAAIRSARAGKKAKGGFV
                     DLLIAQVAAADGCSHTLSFDKAAVRGAGMTLLV"
     gene            1457..2665
                     /locus_tag="VGA59_08995"
     CDS             1457..2665
                     /locus_tag="VGA59_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX9719854.1"
                     /translation="MPPKSQKFALHDHPVQMEPLLPAEHRLAPLLEQAHDLQRAAHGL
                     SATSAPPELRTLLRAMNSYYSNKIEGQHTLPVDIERALRNDYSQDQDKARRQRLALAH
                     METEKALETGWKEWSSGQVWSAQMVRDIHQDLFARLPEADRVLSEGAVLEPGALRDRE
                     VSVGVHAAPAHAAIGAMLDRWAAFYGGVRRGELQIVAVAASHHRLAWIHPFRDGNGRV
                     ARLHTHLALGALGLTNGLWSPLRGFARSHEAYYARLAAADEPRAGDLDGRGNLSERAL
                     EAWIAYVLALCIDQVRFMAGLLDMAGMKERIAACLAFEEKIVRQGVRTEALRALHYLL
                     LAGAELERSEFKAMLGLGDRLATAQVSALLKRGLLVTDSPHGKLRFGVPQHALRFYFP
                     HLWPEAEAGS"
     gene            complement(2774..3907)
                     /locus_tag="VGA59_09000"
     CDS             complement(2774..3907)
                     /locus_tag="VGA59_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HEX9719855.1"
                     /translation="MQSPQYIWQRPGWPKLTFDTVRVAQEVALARRAQGVVEGKVTAL
                     RFQERQELAAEAWAQDAVSTAAIEGEHLDLLTVRSSVARRLGTADQKGPSAPRHVDGL
                     LDIMDDAVTRANEPLTHERLHAWQAALFPTGFSGMRPVKTAAYRPGAMRIVSGRAGNE
                     TVHYAAPAAPEVPGQMQRLLDWFNAGDGLDSIVKAALSHVWFETIHPYEDGNGRVGRN
                     MVDLCLARDAGDTSRLVRISQRLLEQRDAYYDELGQAQHGDGDVTAWVVWFVAQVRAA
                     WQNASEVVEASLEKARFWARHAALELTARQRKAVNALLDRGPGGFEGGMNTRKYVSLT
                     GTSRPTASRELIELNALGVLKQVGGGRSTRYYINLPGWVSETA"
BASE COUNT          664 a         1284 c         1350 g          677 t
ORIGIN      
        1 cgaagaatgc aaggacgacc cacagggtgg agcttgcaga gggttcctgc gcgccgcact
       61 ttacggatgt ctttccgcgc tgccgctccc gcatgtaatc tctatcggcg agtcccattt
      121 gtgcctcctc tcaagggact atacagagtg agcgtcgcgg accgggcgat ggtcacgcgc
      181 gcggcgagcg cacactggtg gtttccgcgg cgctctcgct ggccggcgcc gccatggggt
      241 tgcgcctgaa ggggcgcggg cgccagggca aaacggccgt tatccaagga gtcaattcat
      301 gaagacagtg atggtgcgct acaaggtcaa gccggaatcc gccgccgaga acgagggcct
      361 catcaagcag gtcttcgtgc agctggcccg cgacaagccg gagcgcatgc gctaccaggt
      421 gttcaagcag gccgatggcg tgagcttcgt gcatgtgtcg gcgttcgaca acccggatgg
      481 aaacccgctc acgcagctgg aggcgttcaa ggcgttcacg gccggcatca aggaccgctg
      541 cgcggagccg ccggtgacgg tggagttgca gcaggtgggg cggtacgacc acctggcttg
      601 aggatgaaag agccagggcc agcccatcgc gcccggcgta taattcctta ccatacgtaa
      661 ggagcgtgtt catgcccact gcaacgatga ccagcaaagg ccagatcacg gtacctcagg
      721 ccgtgcgctc cgcattggac ctgcacgctg gtgccaaggt cgactttgtg ccggtcgccg
      781 gcggtttcaa ggtggtaccg ctgcgtgcgg gcagctcggc gctcaaaggc cggtttgccg
      841 gacgcgtggc aaagccggtc agcgtcgaac aaatggacga agccatcgcc gccgaggccg
      901 cccggcgcca cggcagcggg aaacgctgaa cgatgatcgg cctggacacc aatgtgctgg
      961 tgcgctactt ggcacaggac gagccgaaac aagcggcctt ggccacccgc ctgatcgagg
     1021 agcggctgag cgccgcaacg cctggcttca tcagcctggt cgtgctggcg gagctgtgct
     1081 gggtgctcaa gagcctttac ggtgcgagcc aaagcgagct ggccgcgacc gctgaagacc
     1141 tgctgagcgc ggcgcagttt cacctggagc gccgcgaagt gattcaggcc gcaatacgga
     1201 gtgcgcgcgc cgggaagaaa gcgaagggtg gctttgtcga tcttctgatt gcacaagtag
     1261 cggctgcgga tggatgctcg cacacactta gcttcgacaa ggcggcagtg cgtggtgctg
     1321 gcatgacctt gttggtataa gcatcagggt ttggcagcgg ggccggccgc ctggctaccg
     1381 cacgtcaact cgcacgtaaa gtcgcaaaaa ctctggcaga gattgcctat tgttcttgca
     1441 aaacatagac ttaggcatgc ctcccaagtc gcaaaaattc gcgctgcacg accaccccgt
     1501 ccaaatggag cccttgctgc ccgccgagca ccggcttgcg ccgctgctgg agcaggcgca
     1561 cgacctgcag cgtgccgcgc acggcctttc ggccaccagt gcgccgccgg aattgcgcac
     1621 gttgttgcgg gccatgaatt cctattacag caacaagatc gaggggcagc acacgttgcc
     1681 cgtggacatc gagcgcgcgc tgcgcaatga ctattcccag gatcaagaca aggcgcggcg
     1741 gcagcggctg gcgctggcgc acatggagac cgagaaggcg ctcgaaaccg gctggaagga
     1801 gtggtcgagc ggccaggtct ggtcggcaca gatggtgcgc gacatccacc aggacttgtt
     1861 tgcccggctg cccgaggcgg accgggtgct gtccgagggc gctgtgctgg aacccggcgc
     1921 actgcgcgac cgcgaagtca gcgttggggt tcatgccgct cccgcgcacg ctgccatcgg
     1981 cgccatgctc gatcgctggg ccgcgttcta cggaggcgtg cggcgcggcg agctgcaaat
     2041 cgtcgccgtg gcggcatcac accacaggtt ggcctggata cacccgtttc gcgacggcaa
     2101 cggccgggtg gcgcggctgc acacccacct ggccctgggc gctctgggcc tgaccaatgg
     2161 gttgtggtcg cctttgcggg gcttcgcccg cagccatgag gcctactatg ctcgcctggc
     2221 cgccgcggac gagccgcgtg ccggcgatct cgatgggcgt ggcaacctct cggagcgcgc
     2281 gctggaggca tggatcgcgt atgtgctggc gctttgcatc gatcaagtgc gtttcatggc
     2341 cggcctgctc gatatggcgg gcatgaagga gcgcatcgcc gcatgcctgg ccttcgaaga
     2401 gaagatcgtg cgccagggcg tgcgcaccga ggcgttgcgg gcgctgcact acctgctgct
     2461 ggcgggcgcc gagttggagc gcagcgaatt caaggccatg ctgggcctgg gcgatcggct
     2521 ggccacggca caggtgtcgg cgctgctcaa gcgggggctg ctggtgacgg attcgccgca
     2581 cggcaaactg cgcttcggcg tgccgcaaca tgcgctgcgc ttttattttc cgcacctgtg
     2641 gccggaggcc gaggcgggga gctgactcgc gcagtgcagc gggtcgatcg cgcctctgcc
     2701 tagttcttcc cgcgtcttcc cgcgcgcccg accattcaga ccacggggcc ccaactcctt
     2761 acctaccagg cgttcacgcc gtctcggaca cccagccggg aagattgatg tagtagcggg
     2821 tcgagcggcc gccacccact tgcttgagca cgcccagggc gttgagctcg atcagctcac
     2881 gcgacgccgt cggccgggag gtgccggtga ggctgacata cttgcgcgtg ttcatgccgc
     2941 cttcgaagcc gccggggccc cggtccaaca gtgcgttgac cgccttgcgc tggcgcgcgg
     3001 tcagttccag ggctgcgtgg cgcgcccaga acctggcctt ctccagagag gcttcaacca
     3061 cctcggaagc gttctgccag gctgcgcgca cctgcgcgac gaaccagacc acccaggcgg
     3121 tgacgtcgcc gtcgccgtgc tgcgcttggc cgagctcgtc gtagtacgcg tcgcgctgct
     3181 cgagcaggcg ctgggagatc cgcaccagcc ggctggtgtc gccggcgtcc cgcgccaggc
     3241 acaggtccac catgttgcgc cccacgcgcc cgttgccgtc ctcgtagggg tggatggtct
     3301 cgaaccacac gtgcgacagc gccgccttca cgatgctgtc caggccgtcg ccggcgttga
     3361 accagtcgag cagccgctgc atctggccag gcacctccgg cgccgccggg gccgcgtagt
     3421 gcaccgtctc gttgcccgcg cggccgctca cgatccgcat cgcgccgggt cggtaggccg
     3481 cagttttcac cggccgcatg ccggaaaagc ccgtggggaa gagagcggcc tgccaggcgt
     3541 gcaggcgctc gtgcgtgagc ggctcattgg cccgggtgac cgcgtcgtcc atgatgtcca
     3601 agagcccgtc cacgtggcgt ggcgcgctgg gccccttttg gtcagccgtg ccgagacgcc
     3661 gggccaccga tgaacgcaca gtcagcaggt ccaggtgctc gccctcgatc gccgcggtag
     3721 acaccgcgtc ctgcgcccag gcctcggcgg ccagctcctg gcgctcctgg aagcgcagcg
     3781 cagtcacttt gccttcaacg acgccttggg cgcgccgggc cagcgcaacc tcctgcgcca
     3841 cccggacggt atcgaaggtg agcttgggcc agccaggccg ctgccagatg tattgaggcg
     3901 attgcacggc ctattcgact cagtgtttga gttgattata gcggctattc gactcatttt
     3961 gcgagtcgaa taaac
//
LOCUS       DASWIY010000245         3092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_893901, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000245 DASWIY010000000
VERSION     DASWIY010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893901"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1543)
                     /locus_tag="VGA59_09005"
     CDS             complement(152..1543)
                     /locus_tag="VGA59_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HEX9719856.1"
                     /translation="MTNASAASRAPVFERIGERVAGNVQKWLPDPFLFAILLTFIAFI
                     MGMVFADKSAEQMVRHWYSGFWNLHTFAMQMVLILVTGYVLAHAPFVRPALNWLADQP
                     RTGGQAVVLVTVLALVFSWINWGFGLIVGAIMAVEVGKRAHAKRIPVHYPVLCTAGYA
                     GLGVSWHWGPSGSAPLLSATKGHIFEKLIGVVPTSDTIFSGYAIWLSILSLIFVPLIL
                     YLIAPKTADRCRGIEHWLPEAVKGATGKEAAAASKGDASWADRINNSLILGGLIALMG
                     LIYIVMHFWRGGTLDLNIVIFIFLIVGLALYVRPYQYLLAFYEAVRASAGVILQFPFY
                     AGIMGMIGLSGLGVIMAGWLINIANPTTFPMMAWLASGFVNLFVPSGGGEWAVLGETI
                     LRAGQSLGVPVGKTIIAFATGDAWTNLFQPFWAIALLGLTGMRARDMFGYCITLMFAL
                     LPFFAILLTWLPY"
     gene            complement(1684..>3092)
                     /locus_tag="VGA59_09010"
     CDS             complement(1684..>3092)
                     /locus_tag="VGA59_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9719857.1"
                     /translation="NGPAMYEAHFGVPMAGAVLNTINTRLDADTIAFILQHGEARVLL
                     TDREFSATVEKALEIAKARLLVIDVDDPEFIGGKLIGETDYEKFLLSGDPEFPWRIPD
                     DEWDAISLNYTSGTTGNPKGVVYHHRGAYLNAISNILSWGMPPHPVYLWTLPMFHCNG
                     WCFPWTIAAAAGTNVCLRRVDAKMIFDFIRKYRVTHYCGAPIVHSQLLNAPAEWKQGI
                     SHTVCCLVAGAAPPVAVIEGMEKMGFNITHVYGLTETYGPAAVCAKHPEWDALPLEQR
                     AELIGRQGVWYHLQQGMTVLDPATMNAVPWDGAAMGEIMFRGNLTMKGYLKNPKATQE
                     AFSGGWFHSGDLAVMQADGYVKIKDRSKDIIISGGENISSLEVEDVLYRHPAVMAAAV
                     VAQPDPKWGEVPCAFVELKDGVKASEAEIIEHCRGRMAHFKAPRRVVFGPLPRTSTGK
                     IQKFILREQVKSASAIDI"
BASE COUNT          589 a          988 c          992 g          523 t
ORIGIN      
        1 tccaccggtg attccacttc ggaatgcggg cacgcccacg aggaggacgg caccctgccc
       61 gacgggcgat acggggagcc gcggccggcg gattaccgca cgcgcgggga ctgagcggtt
      121 ccgaccgact gccagggcga cgccgatccg ttcagtacgg cagccacgtc agcagaatcg
      181 cgaagaacgg cagcagcgcg aacatcaggg tgatgcaata gccgaacatg tcgcgcgcgc
      241 gcatgccggt gaggccgagc agcgcgatcg cccagaacgg ctggaacagg ttcgtccagg
      301 catcgccggt ggcgaaggcg atgattgtct tgccgaccgg cacccccagg ctctgtccgg
      361 cgcgcaatat ggtctcgccg agcacggccc attccccgcc acccgacggc acgaacaggt
      421 tgacaaaccc cgaagcgagc catgccatca tcgggaaggt ggtggggttg gcgatgttga
      481 tcagccagcc cgccatgatg acgcccagcc cggagaggcc gatcatgccc atgatccccg
      541 catagaacgg gaactgcagg atgacgccgg cggaagcgcg aaccgcttca tagaaggcga
      601 gcaggtactg gtaaggccgc acgtaaaggg cgagacctac gatcaggaag atgaagatga
      661 cgatgttgag atcgagcgtg ccgccgcgcc agaagtgcat cacgatatag atgaggccca
      721 tcaaggcgat gagcccgccg aggatcaggc tgttgttgat gcggtcggcc cagctcgcgt
      781 cgcctttcga cgcggccgcg gcctctttgc ccgtggcccc cttcacagct tcgggcagcc
      841 agtgctcgat gccgcggcag cggtcagccg tcttgggcgc aatcaggtac aggatcagcg
      901 gaacaaagat caggctcaag atcgacagcc agatcgcgta gccggagaag atggtatccg
      961 acgtcggcac gacgccgatc agcttctcga agatgtgccc cttggtggcg ctcaggagcg
     1021 gcgccgatcc ggacggcccc cagtgccagc tcacgccgag cccggcgtag ccggcggtgc
     1081 acagcaccgg atagtggacc ggaatgcgct tggcgtgcgc gcgctttccc acctcgaccg
     1141 ccatgatggc gccgacgatg agcccgaagc cccagttgat ccaggaaaag acgagcgcca
     1201 gcaccgtgac cagcaccacg gcctgtccgc cggtcctggg ctggtcggcg agccagttca
     1261 gtgccggccg cacgaatggc gcgtgggcca gcacgtaacc cgtgacaaga atcaggacca
     1321 tctgcatggc gaaggtgtgg agattccaga acccgctgta ccagtgccgc accatctgct
     1381 ccgccgactt gtcggcgaag accatgccca tgatgaaggc gatgaaggtg agcaggatcg
     1441 cgaacagaaa cgggtccgga agccacttct gcacgtttcc ggcgacgcgc tccccgatcc
     1501 gctcgaacac gggtgcgcgt gaagcagcag atgcgtttgt catgacggtc cctcccccga
     1561 aggaattcca gccatttcgc acctgatgga gtccactgtc aagaggcgct gaattcttgc
     1621 gcccattgaa taacatgcgt ccgcgcgggg ttcaaactgg gcgcgtggcg tgctcctgcc
     1681 gcgtcagatg tcgatcgcgg aggcggactt cacctgctcg cgcaggatga atttctggat
     1741 cttgccggtg gaggtcctgg gcagcggtcc gaacaccacg cgccggggcg ccttgaagtg
     1801 cgccatgcgg ccgcggcagt gctctatgat ttcagcctcg ctcgccttca cgccgtcctt
     1861 cagctcgacg aaggcgcacg gcacctcgcc ccatttggga tcgggctggg ccaccaccgc
     1921 cgccgccatc acggcgggat gccggtagag cacgtcctcg acctcgagcg aggagatatt
     1981 ctcgccaccc gagatgatga tgtccttgga gcggtccttg atcttgacgt agccgtccgc
     2041 ctgcatcacc gccagatcgc cgctgtggaa ccacccgccg gagaacgctt cctgcgtggc
     2101 cttcgggttc ttgagatagc ccttcatggt gaggttgccg cgaaacatga tctcgcccat
     2161 ggccgcgccg tcccacggca cggcgttcat ggtcgcggga tccagcaccg tcatcccctg
     2221 ctgcaggtgg taccacacgc cctggcggcc gatgagctcg gcacgctgct ccagcggcaa
     2281 cgcgtcccac tccggatgct tcgcgcatac ggccgcgggt ccgtaggtct cggtgagccc
     2341 gtagacatgg gtgatgttga agcccatttt ctccatgcct tcgatcactg ccaccggcgg
     2401 cgccgcgccc gccaccaggc aacatacggt gtggctgatg ccctgcttcc attcggcagg
     2461 ggcgttgagc agctggctat gcacgatggg cgcgccgcaa tagtgggtga cgcgatactt
     2521 gcggataaaa tcgaagatca tcttcgcgtc cacccggcgc aagcacacgt tggtgccggc
     2581 cgcggcggcg atggtccacg ggaaacacca gccgttgcag tgaaacatcg gcagcgtcca
     2641 caggtagacc gggtgcggcg gcatgcccca ggagagaatg ttgctgatgg cgttgagata
     2701 ggcgccgcgg tgatggtaaa ccacgccttt gggattgccg gtggtgcccg aggtgtaatt
     2761 gagggagatc gcatcccact catcatccgg aattcgccaa gggaattccg gatcacccga
     2821 aagcaggaat ttctcgtagt cggtctcccc aatcaatttt ccgccgatga actccggatc
     2881 atccacatcg atcaccagca gcctggcctt ggcgatctcg agggctttct cgaccgtggc
     2941 ggaaaactct cggtcggtga gcagcaccct cgcttcgccg tgctgcagga tgaaggcgat
     3001 ggtgtcggcg tcgagccggg tgttgatggt attgagcacc gcgcccgcca tcggcacccc
     3061 gaagtgcgct tcgtacatcg ccggcccgtt gg
//
LOCUS       DASWIY010000246         4822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2749790, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000246 DASWIY010000000
VERSION     DASWIY010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2749790"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VGA59_09015"
     CDS             complement(<1..309)
                     /locus_tag="VGA59_09015"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427598.1"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; catalyzes CMP phosphorylation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HEX9719858.1"
                     /translation="MFPTEFLDIPPLQGAAGTLPLPGSKSISNRVLLLAALSGGTTTV
                     HDLLDSDDTRVMLNALRTLGCGVRHSGAAVEITGLGGRAPASHARLFLGNAGTAMRPL
                     A"
     gene            complement(335..1207)
                     /locus_tag="VGA59_09020"
     CDS             complement(335..1207)
                     /locus_tag="VGA59_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX9719859.1"
                     /translation="MFEQLGLIGCGLMGGSFALALKRAGLVKRVVGYSKSPSTTERAR
                     QMGVIDVEAPSALLAVSGADIVLLAVPVAATEATLKAIRHLVTKDMLIMDVGSTKRDV
                     IDSARRVLRDHIGSFVPCHPVAGKELSGVDHADPDLYTGKQVILTPIDRTEAKQLQDA
                     VTLWEALGCRVLKMAPEAHDAAFAAVSHLPHLIAFAMINAVAGQPQGTEYLSLAGPGF
                     RDFTRIAASDPKMWRDILLANRHELLAQAQIFQRTLNQMEQLIESGDGEALEGLLAQA
                     SGARAQWRMGASKK"
     gene            complement(1282..2364)
                     /gene="pheA"
                     /locus_tag="VGA59_09025"
     CDS             complement(1282..2364)
                     /gene="pheA"
                     /locus_tag="VGA59_09025"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX9719860.1"
                     /translation="MAHDLNAIRTEIDAIDRQLLQLLNRRAALANEVGEIKRAEGSTV
                     FRPEREAQVINGLQAANPGPLKGSNVATIWREIMSACRALEAPQRVAYLGPVGTFSEQ
                     AAVQFFGSSIEHVPCVSFDEVFHAATAGTADYGVVPVENSSEGVVTRSLDLLLNSPLH
                     IVGEISLLVRHHLLRLAGSVEGIEVVLAHPQALAQCQSWLNKHLPHAERRAVSSNAEG
                     ARLAAGNPAWAGIGSERAASEFGLHVAAHAIQDDAFNRTRFAVVCLPQVLDAPQASGR
                     DCVSLVVSVPNRPGAVHDILVPLKRHGVSMTRFESRPARSGQWEYYFYIDVQGHPSQS
                     NVAAALKDLQSLCAFYKVLGTYPVGE"
     gene            complement(2364..>4822)
                     /gene="gyrA"
                     /locus_tag="VGA59_09030"
     CDS             complement(2364..>4822)
                     /gene="gyrA"
                     /locus_tag="VGA59_09030"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX9719861.1"
                     /translation="YKKSARIVGDVIGKYHPHGDQSVYDTIVRLAQDFSMRHMLVDGQ
                     GNFGSVDGDNAAAMRYTEIRLAKIAHEMLADIDKETVDFGPNYDGSEKEPLVLPSRLP
                     NLLVNGSGGIAVGMATNIPPHNLNEVVDACLHLLRNPEASIDELMEIVPAPDFPTAAI
                     IYGINGVKEGYRTGRGKVVMRAKCHFEDIDRGQRQAIIVDELPYQVNKKTLQERMAEL
                     VNEKKIEGISHIQDESDKSGMRLVIELKRGEVPEVVLNNLYKQTQLQDTFGINMVALI
                     DGQPRLCNLKDLVEVFLQHRREVVTRRTVFALRKARERGHVLEGLAVALANIDEFIAI
                     IRNAPTPPVAKSELMSRPWDSKLVREMLTRTRADGGVVNADDYRPDALDRAFGMGGDG
                     LYRLSDTQAQEILQMRLQRLTGLEQDKIVAEYKEVMAEIDDLLDILAKPARVSTIIGE
                     ELAALKHEFGQTKLGARRSVVEHNAQDLATEDLITPTDMVVTLSHAGYIKSQPVSEYR
                     AQKRGGRGKQATATKEDDWIDQLFIANTHDWILCFSNRGRMYWLKVWEVPAGSRGSRG
                     RPIVNMFPLAEGEKINVVLPLTGEFRSFPADHYVFMATSMGTVKKTALDEFSNPRKAG
                     IIAVDLDDGDYLIGAALTDGKHDVMLFSDGGKAVRFDENDVRPMGRAARGVRGMMLDD
                     GQGVIAMLVAEDEQQSVLTATEKGYGKRTSITEYTRHGRGTKGMIAIQQSERNGKVVA
                     ATLVHSDDEIMLITDKGVLVRTRVSEIRELGRATQGVTLIGLDEGSKLGGLQRIVEND
                     AAAVEAEGDAPDGAGPEGEG"
BASE COUNT          725 a         1576 c         1616 g          905 t
ORIGIN      
        1 cgccaggggc cgcatcgcgg ttccggcgtt gcccaggaac agcctggcat gcgacgccgg
       61 cgcccggccg cccaggccgg tgatctccac ggctgcgccg ctgtgcctga cgccgcagcc
      121 cagggtgcgc aaggcattga gcatgacccg ggtgtcgtcc gagtcgagca ggtcgtggac
      181 cgtggtcgtt ccgccgctca acgcggccag cagcagtacg cggttggaaa tgctcttgga
      241 accgggcagc ggcaaagtcc ccgcggcgcc ctgaaggggc ggaatgtcga ggaattcggt
      301 ggggaacatc aggcctggcc ccgcggggtc cggatcattt cttcgacgca cccattcgcc
      361 actgcgcgcg cgccccgctc gcctgggcga gcagcccctc cagcgcctcg ccgtcaccgc
      421 tttcgatcag ctgttccatc tggttcaagg tgcgctggaa aatctgagcc tgggccagca
      481 gttcgtgccg gttggccaga aggatgtcgc gccacatctt gggatcgctg gccgcgatcc
      541 gcgtgaaatc ccgaaagccc gggccggcga gcgaaaggta ttccgttccc tgcggctggc
      601 cggcgaccgc gttgatcatg gcgaaggcaa tgaggtgggg caggtggctc acggccgcga
      661 acgccgcgtc gtgtgcctcg ggcgccatct tcaatacccg gcacccgagg gcctcccaca
      721 aggtgacggc gtcctgcagc tgcttcgctt cggtgcggtc gatcggggtg aggatgacct
      781 gcttgccggt atagaggtcg gggtcggcgt gatcgacccc ggacaattcc ttgcccgcga
      841 ccggatggca aggcacgaaa gagccgatgt ggtcgcgcag gacgcggcgg gccgagtcga
      901 tcacgtcgcg tttggtcgag ccgacgtcca tgatcagcat gtccttggtg accaggtgcc
      961 tgatcgcctt gagcgtggct tcggtggccg ccaccggcac cgcgagcagg acgatgtccg
     1021 cgcccgacac ggccagcagc gccgacggcg cttccacgtc gatcacgccc atctgccggg
     1081 cgcgctcggt ggtggagggc gacttgctgt agccgaccac ccgcttcacc agcccggcac
     1141 gcttgagcgc gagcgcgaag gaccccccca tgaggccgca gccgatcaga cccagttgct
     1201 cgaacatatt gttgtgatct cagtgtccat tgcgcttcgc gccgtcccat gttcgtgaaa
     1261 gcgcgcggcc agggtgggct ttcattcgcc gaccggatag gtacccagca ccttgtagaa
     1321 ggcgcacagg gattgcagat ccttgagcgc ggccgcgaca ttggactgcg acggatggcc
     1381 ttgcacgtcg atgtagaaat agtattccca ctggcccgag cgggccggcc gcgattcgaa
     1441 ccgcgtcatc gaaacgccgt ggcgcttgag cggcaccagg atgtcgtgca ccgcgcccgg
     1501 cctgttcggg accgatacga ccagactcac gcaatcgcgg cccgaggcct gcggcgcgtc
     1561 caggacttgc ggcaggcaga ccacggcaaa acgcgtgcgg ttgaaggcat cgtcctggat
     1621 ggcatgagcg gcgacgtgca ggccgaattc gcttgccgcc cgttcgctgc cgatgccggc
     1681 ccaggccgga ttgccggcgg ccagccgcgc gccctccgcg ttgctggaca cggcccggcg
     1741 ctcggcgtgg ggcaggtgct tgttcagcca gctctggcat tgcgccagcg cctggggatg
     1801 cgccaacacc acctcgatgc cttcgaccga gccggccagg cgcagcaggt ggtgccgcac
     1861 cagcaggctg atttcgccga cgatgtgcag cggcgaattc agcagcaggt cgagcgagcg
     1921 cgtgaccacg ccctcgctgc tgttctccac tggaaccacg ccgtagtcgg cggtgcccgc
     1981 cgtcgcggca tgaaaaacct cgtcgaagct gacacaggga acatgctcga tgctggagcc
     2041 gaagaactgc accgcggcct gctcgctgaa tgtgccgacc ggtcccaggt aggcgacgcg
     2101 ctgaggcgcc tccagggcgc ggcacgccga catgatctcg cgccagatgg tggcgacgtt
     2161 gctgcccttc agcgggcccg ggttggcggc ctgcaatccg ttgatgacct gcgcttcgcg
     2221 ctcggggcgg aacacggtgg agccctcggc gcgcttgatc tcgccgactt cgttggccag
     2281 cgcagcgcgc cggttcagca attgcaggag ctggcgatcg atcgcgtcga tctcggtgcg
     2341 tatcgcgttc aggtcatggg ccatcagcct tcgccctcgg ggccggcgcc atcgggagcg
     2401 tccccttccg cctcgaccgc cgccgcgtcg ttctcgacga tgcgctgcag tccgccgagc
     2461 ttggaacctt cgtccaggcc gatcagcgtc acgccctgcg tggcccggcc gagttcgcgg
     2521 atctcgctca cgcgggtgcg caccagcacg cctttgtccg tgatgagcat gatctcgtcg
     2581 tcggaatgca ccagcgtggc ggccaccacc ttgccgttgc gctcgctttg ctggatggcg
     2641 atcatgccct tggtgccgcg gccgtggcgc gtgtactcgg tgatgctggt gcgcttgccg
     2701 tagccttttt cggtcgccgt gagcacgctt tgctgctcgt cctcggccac cagcatggcg
     2761 atcacgccct ggccgtcgtc cagcatcatg ccgcgcacgc cgcgagcggc gcgccccatc
     2821 ggccggacat cgttttcgtc gaagcgcacg gccttgccgc cgtcggaaaa gagcatgacg
     2881 tcgtgcttgc cgtcggtgag ggccgctccg atgaggtagt cgccgtcatc gaggtcgacg
     2941 gcgatgatgc cggccttgcg cgggttgctg aattcgtcga gcgccgtctt cttgacggtg
     3001 cccatcgaag tggccatgaa gacgtagtgg tcggccggga agctgcggaa ttcgccggtg
     3061 agcggcagca cgacattgat cttctcgccc tcggccagcg ggaacatgtt gacgatcggg
     3121 cgtccgcgcg atccgcgcga tccggccggg acttcccaga ccttgagcca gtacatgcgg
     3181 ccccggttgg agaagcacag gatccagtcg tgcgtattgg cgatgaagag ctggtctatc
     3241 cagtcgtcct ctttcgtcgc ggtcgcctgc ttgccgcggc cgccgcgctt ttgcgcccgg
     3301 tattcgctca cgggctggct cttgatgtag ccggcatggg acagcgtcac caccatgtcg
     3361 gtgggtgtga tcaggtcttc ggtggcgagg tcctgcgcgt tgtgttcgac cacgctgcgg
     3421 cgggcgccca gcttcgtctg gccgaactcg tgcttgagag cggccagttc ctcgccgatg
     3481 atcgtcgaca cccgcgccgg cttggccagg atgtcgagca ggtcgtcgat ctcggccatc
     3541 acttccttgt actcggcgac gatcttgtcc tgctccaggc ccgtgaggcg ctgcagacgc
     3601 atctgcagga tttcctgcgc ctgggtgtcc gagaggcggt acagcccgtc gccgcccatg
     3661 ccgaacgcgc gatccagcgc gtccgggcgg tagtcgtcgg cgttgaccac gccgccatcg
     3721 gcgcgggtac gggtgagcat ttcacgcacc agtttgctgt cccagggccg gctcatcagc
     3781 tcggatttgg cgaccggcgg cgtgggggca ttgcgaatga tcgcgatgaa ttcgtcgatg
     3841 ttggccaggg ccacggccag gccttccagc acatggccgc gctcgcgggc cttgcgcaac
     3901 gcaaacaccg tgcggcgggt gacgacttcc cggcgatgct gcaggaagac ctcgaccagg
     3961 tctttcaagt tgcacaagcg gggctggccg tcgatcagcg ccaccatgtt gatgccgaag
     4021 gtgtcctgca gctgggtctg tttgtacaga ttgttaagaa ccacctcggg aacttcgcca
     4081 cgcttgagtt cgatgaccag gcgcatgccg gacttgtcgc tttcgtcctg gatatggctg
     4141 atgccctcga tcttcttttc gttgaccagt tcggccatgc gttcctgcaa ggtcttcttg
     4201 ttgacctggt agggcagctc gtccacgatg atggcctggc gctggccgcg atcgatgtcc
     4261 tcgaaatggc acttggcgcg catcaccacc ttgccgcggc cggtgcggta accctccttg
     4321 acgccattga tgccgtagat gatggcggcc gtcgggaagt cgggggcggg cacgatttcc
     4381 atcagctcgt cgatgctcgc ctccgggttg cgcaacaggt gcaggcaggc atccaccacc
     4441 tcgttgaggt tgtgcggcgg gatgttggtg gccatgccca ccgcgatgcc gcccgaccca
     4501 ttgaccagca ggttgggcag tcggctgggc aggaccagcg gctctttttc gctgccgtcg
     4561 tagttgggcc cgaaatcgac agtctccttg tcgatatccg ccagcatctc gtgtgcgatc
     4621 ttggccagcc ggatctcggt atagcgcatg gccgccgcgt tgtcgccgtc gacggaaccg
     4681 aagttgccct ggccgtccac cagcatgtgg cgcatggaaa agtcctgcgc cagccgcacg
     4741 attgtgtcgt agaccgactg gtcgccatgg gggtggtact tgccgatgac gtccccgacg
     4801 atgcgcgccg acttcttgta gg
//
LOCUS       DASWIY010000247         6749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_538798, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000247 DASWIY010000000
VERSION     DASWIY010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538798"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VGA59_09035"
     CDS             <1..200
                     /locus_tag="VGA59_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719862.1"
                     /translation="LQDFDEPIAIKPFNTRILQDNCLACHRALVHEAAMARGRSEEVS
                     CVHCHLSAGHGERAGLGGPRT"
     gene            216..1745
                     /locus_tag="VGA59_09040"
     CDS             216..1745
                     /locus_tag="VGA59_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HEX9719863.1"
                     /translation="MKSRSRNTARAQRGVSTTVLLSSIVVVALGTAAVTALLVNIFER
                     KQEARVATSTRLVEVTQDTTDPAKWGVNWPKQYDSYLLTAQSTRTRFGGHGGSEALPQ
                     QKIDKDPWLKRMFLGYAFSIDYRDRRGHAYMLQDQENTQRQTKPQTGSCLHCHGSVMP
                     LYRQMGGGDAMKGFEMSYKLTYKEMNQKLHDMGHAHTVTCADCHDPKTMQLIVTRPAF
                     LQGIQSLAGSDAATPFAPSIERWRTAGKAKPYDPNTDASRGEMRTFVCAQCHIEYYCS
                     SQMPLTAPWGKGLNVVQIETFWNQTKFADGERFFDYKHAETGAPILKAQHPEFELWSQ
                     GVHARAGVACADCHMPYARDGATKVSDHWVRSPLLNVNRACQTCHKQSEDEIKVRVDL
                     IQTRNFDLLQRGGVAIAALMDAIVAAKKDGATPGQLNAALELQRQAQWRLDFIAAENS
                     MGFHAPQEAAKVLGEAIDYARQGEAAAMRWQTPQAKAAAANADKVAAAGTKPIVAGKL
                     P"
     gene            complement(1800..2360)
                     /locus_tag="VGA59_09045"
     CDS             complement(1800..2360)
                     /locus_tag="VGA59_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX9719864.1"
                     /translation="MSRWLCIFLLVACTGARAVPGAPAEDDMASFMADRGLLTTLEQV
                     SHTVTDRASDLVVTAMGFLGVPYRRGGNSAETGFDCSGFVRAMYERTVGLLLPRRADE
                     QAAATQAIDRKDLQPGDLVFFNTMKRAFSHVGIYVGEGKFIHSPRPGAQVRVEDMGQN
                     YWQRRFDGARRVLNLSMTQPQLQAQP"
     gene            2554..3651
                     /locus_tag="VGA59_09050"
     CDS             2554..3651
                     /locus_tag="VGA59_09050"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX9719865.1"
                     /translation="MRSFVVITFAAFGWIPVSADVRVDTIASGLEHPWAVAFLPKGRF
                     LVTERPGRMRVVAADGKVLPAIAGVPQVEAGGQGGLLDVVLDSDFTANRVLYFCYSEP
                     GQGGNSTALARARLSADESRLEDMKVIFSQKPKVASRLHFGCRIVETRDGFLFLTLGE
                     RFQRKDDAQKLDNHLGKIVRLAKDGSPAAGNPFASRAGALPEIWSYGHRNSQGATAGP
                     DGRLWMHEHGPQGGDEINLPQPGRNYGWPVITYGENYGGGKIGEGLTAGPGMEQPLHY
                     WVPSIAPSGMAFLTSDRYGPGMKGNLFIGSLKFGYLARLELSGGKVVREHKLLSGAGR
                     VRDVRQGPDGLLYVLTDSARGSLLRLLPPDK"
     gene            complement(3733..4380)
                     /locus_tag="VGA59_09055"
     CDS             complement(3733..4380)
                     /locus_tag="VGA59_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9719866.1"
                     /translation="MKNTAASQPVRIVLADDHDLVRSGIKALLTMIEGVEVIAEARDG
                     EELIALVDSLMPDVVMTDISMPGMDGITAISHIHGKHPQARLLVLSMYDTVDFVKRAV
                     ASGACGYLMKDAPPFELEQAVRSVMATGSYFSPAIAQRLLQPSEPTVDDELTHRQVEI
                     LRLIAQGRASKEIAYELGLSPKTVDVHRARIMERLHLNDIASLTLYAVRKGLIKP"
     gene            complement(4396..5484)
                     /locus_tag="VGA59_09060"
     CDS             complement(4396..5484)
                     /locus_tag="VGA59_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9719867.1"
                     /translation="MTNPAEPATIRLLCVEDSPDDVELMAIALERADPQRRYVLHRVE
                     DASGFTDALQQDFDVILCDFNMPRFSPYAALQILVARRCGTPLVVVTRAIGEEAAVHV
                     LRCGAKDYLTKDKLGTLPQIIERVMTERRRVLEQERMARELEAAYRRLKKLSARLVVA
                     QERERTLISRELHDQLGQTLTGMVIHLHAAKRAADPAAVHQHTETAMDMAQAAVKQLK
                     TLSFTLRPAQLDLLGLVAATHTAVQRIAEPAELAFSVSSRGQEPPVLGETASVALRLV
                     QEALNNVVRHAQASHIAVRLRFLPQARIGVLVVDDGVGFDKHRILDGQAGEHNVGLYG
                     MIERTELAGGRLHIRTYPGRGVAIRAVL"
     gene            5661..6248
                     /locus_tag="VGA59_09065"
     CDS             5661..6248
                     /locus_tag="VGA59_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719868.1"
                     /translation="METLDTQTLAIVAVVILALVAVGAWIIYNRKQQSNRLMQRFGPE
                     YGRAVDQMGSRAKAEAELRSREKRVEKFRIVPLAPADAARFSQDWKTLQGRFVDNPKG
                     VLVEADMLVRELMEKRGYPMGDFESRAADISVDHPAVVDHYRAAQAIAMRDRRGEADT
                     EEMRKAVVHYRALFDELLEVRQDERAVTREAEVHS"
     gene            6245..6676
                     /locus_tag="VGA59_09070"
     CDS             6245..6676
                     /locus_tag="VGA59_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719869.1"
                     /translation="MNTVHVEDRDKTLTTADVAAAAQRPQAQPDLNRTGTVDRSTDLR
                     QQPAVGGDEQLAALFATDMADDFRSRWDAVQIGFVDDPRQAVRQADELVAEVMKSLAE
                     SFSAERDRLESQLEQQDSASTENLRVALRRYRSFFQRLLSL"
BASE COUNT         1224 a         2172 c         2248 g         1105 t
ORIGIN      
        1 ccctgcagga cttcgacgaa cccattgcca tcaaaccgtt caacacccgc atcctgcagg
       61 acaactgcct tgcctgccat cgcgcgctgg tgcacgaggc ggcgatggcg cgggggcgca
      121 gcgaggaagt cagctgcgtg cactgccatc tgtccgcggg gcacggcgag cgcgccggcc
      181 tgggtggacc gcgaacctga agctcggagg ctgtcatgaa atccagatcc cgcaacaccg
      241 ctcgcgcgca gcgcggcgtt tcgacaacgg tcctgctgtc gagcatcgtt gtggtggctc
      301 tcggcaccgc cgcggtcacc gcgctgctgg tcaacatctt cgaacgcaag caggaagcca
      361 gggtggccac cagcacgcgg ctcgtggagg tgacccagga caccaccgat ccggccaagt
      421 ggggcgtcaa ctggcccaag cagtacgact cgtacctgct gacggcgcag tcgactcgca
      481 cgcgcttcgg cggccacggc ggcagcgagg cactgcccca gcagaagatc gacaaggacc
      541 cgtggctcaa gcgaatgttc ctgggctacg ccttctcgat cgactatcgg gaccgccgcg
      601 gccacgccta catgctgcag gaccaggaga acacgcagcg ccagaccaaa ccgcaaaccg
      661 gctcgtgcct gcattgccac ggatcggtga tgcctttgta ccgtcagatg ggcggcggcg
      721 atgcgatgaa gggcttcgag atgagctaca agctcaccta caaggagatg aaccagaagc
      781 tccacgacat ggggcacgcc catacggtga cctgcgcgga ctgccacgac ccgaagacga
      841 tgcagctgat cgtcacgcgg ccggccttcc tgcaaggcat ccagagcctg gccggctcgg
      901 acgccgccac gcccttcgcg ccttcgatcg agcgctggcg caccgccggc aaggccaaac
      961 cctacgaccc caacaccgac gccagccgcg gcgagatgcg cacgttcgtg tgtgcccaat
     1021 gccacatcga atactactgt tcgtcgcaaa tgccgctgac cgcgccgtgg ggcaaggggc
     1081 tgaatgtggt gcagatcgag acgttctgga accagacgaa attcgccgac ggtgaacgct
     1141 tcttcgatta caagcacgcc gaaaccggcg ccccgatcct gaaggcccag catcccgagt
     1201 tcgagctgtg gagccagggc gtgcatgcgc gcgccggcgt cgcctgcgcg gactgccata
     1261 tgccgtacgc ccgcgacggc gccaccaagg tgtccgacca ctgggtgcgc agcccgctgc
     1321 tgaacgtcaa ccgcgcctgc cagacctgcc acaagcagtc cgaggacgag atcaaggtgc
     1381 gcgtcgacct gatccagacg cgcaacttcg atctgctcca gcgcggcggt gttgcgatcg
     1441 ccgcgctgat ggatgcgatc gtggcggcga aaaaagacgg tgccacgccg gggcaattga
     1501 acgcggcgct cgagttgcag cgccaggcgc aatggcggct cgacttcatc gcggcggaga
     1561 attccatggg cttccatgcg ccgcaggaag ccgccaaggt cctgggcgaa gcgatcgact
     1621 atgcgcgcca gggtgaagcg gcggcgatgc gctggcaaac gccgcaagcc aaagctgcag
     1681 cggcgaacgc agacaaagtc gcggcggcgg ggacgaagcc catcgtcgcc ggcaagcttc
     1741 cctgagcggc cggcagccgg ttgccgcacg gattcaggaa gggctgaaaa cccgcccggt
     1801 cagggctgcg cttgcagctg cggctgggtc atggacagat tcagtacccg ccgggcgccg
     1861 tcgaagcgcc gctgccagta gttctgcccc atgtcttcga cccggacctg ggcgcccggc
     1921 ctgggggaat ggatgaactt gccttcgccg acatagatgc ccacgtggct gaaagcgcgc
     1981 ttcatcgtgt tgaagaacac caggtctccc ggctgcaggt ccttcctgtc gatggcctga
     2041 gtggcggccg cctgctcatc ggcgcgacgc ggcagcagca ggccaacggt ccgctcgtac
     2101 atggcccgaa cgaaaccgct gcaatcgaaa cccgtttcgg cggagttgcc accgcgccgg
     2161 tagggcacgc cgaggaagcc catcgccgtg accacgaggt ccgaagcccg gtcggtgacg
     2221 gtatggctca cttgctccag ggtagtcaac aagcccctgt cggccatgaa actggccatg
     2281 tcgtcctcgg cgggcgcgcc cgggacggca cgcgcgccgg tacacgccac gagcaggaaa
     2341 atgcagagcc accttgacat ggccgcgagc ataccctcgc atgagctgag aagtcaactt
     2401 aggtatggat cgtaacttgt tgattcctat gtggaagagc cctgcgttta tgtctggaaa
     2461 ctgtgcgggc cgcgaagttc gggaagaatg cacgctgtca gccatccagg ttgttgggag
     2521 cccccgatgc aaggcgtgat gttgcagcgc gcgatgcgca gtttcgtcgt catcactttc
     2581 gccgctttcg gttggatacc ggtctcggcg gatgtgcgtg tggatacgat tgccagcggg
     2641 ctggagcacc cctgggcggt cgcgttcctt ccgaaagggc gcttcctcgt cacggagcgg
     2701 ccgggccgga tgcgtgtggt cgcggccgac ggcaaggtcc tgccggcgat tgcgggtgtg
     2761 cctcaggtgg aggccggcgg acagggcggg ctgctcgacg tggtgctgga ctcggatttc
     2821 acggcaaacc gggttctcta tttttgttac tcggaacctg gacaaggcgg caacagcacg
     2881 gccctggccc gggccaggct ctcggcggat gaatcccgtc tggaggatat gaaggtgatc
     2941 ttcagccaga agcccaaagt cgcgagcaga ctgcacttcg ggtgccgcat cgtcgaaacg
     3001 cgcgacggct tcctcttcct gaccttgggc gagcgcttcc agcgcaagga cgacgcgcag
     3061 aaactggaca accacctggg caagatcgtc cggctggcca aggacggctc gcccgcggcg
     3121 ggcaatcctt ttgcctcccg ggccggcgcg ctgcccgaaa tctggagtta cggccaccgc
     3181 aattcgcaag gtgccactgc ggggcccgac ggcagactgt ggatgcatga gcatgggccg
     3241 caggggggcg acgaaatcaa cctgccgcag ccggggcgca actacggctg gccggtcatc
     3301 acctatggcg aaaactacgg cggcggcaaa atcggggagg ggctgacggc cggcccggga
     3361 atggaacagc cgctgcatta ctgggtaccc tcgatcgcgc cgtcgggcat ggctttcctg
     3421 accagcgacc gctacggtcc gggcatgaaa ggcaatctat tcatcgggtc gctcaaattc
     3481 gggtacctgg cgcggctgga actgtccggc ggcaaggtgg tgcgcgagca caagctgctg
     3541 tccggcgccg ggcgggtgcg cgatgtgcgc cagggtccgg acggcttgct gtacgtgctc
     3601 acggacagcg cgcgcggcag ccttttgcgg ctgctgcctc cggacaagtg acaaatgaaa
     3661 gatggcaaat gacaaatgcc aaagggagcg cgaggaactc ctttgtcatt gtccattcgt
     3721 cattgcattc ccttacggct tgatcagccc cttgcgcacc gcgtacaggg tcaggctggc
     3781 gatgtcgttc aggtgcagcc gctccatgat gcgggcgcgg tgcacgtcca cggtcttggg
     3841 cgacaggccc agttcgtacg cgatttcctt ggaagcccgt ccctgggcga tgagccgcag
     3901 gatttcgacc tgccgatggg tgagctcgtc gtccaccgtg ggttcggatg gctgcaacag
     3961 gcgctgggcg atggcggggc tgaagtagct gccggtggcc atcacgctgc gcaccgcctg
     4021 ttccagctcg aacggcggcg catccttcat caggtagccg caggctccgc tggccaccgc
     4081 gcgcttgacg aagtcgaccg tgtcgtacat cgaaagcacc agcagcctcg cctgcgggtg
     4141 cttgccgtgg atatgcgaaa tcgccgtgat gccgtccatc ccgggcatgg aaatgtccgt
     4201 catgaccacg tcgggcatca ggctgtccac cagggcgatc aattcctcgc cgtcgcgggc
     4261 ttccgcgatc acctcgacgc cctcgatcat ggtcagcagc gccttgatgc ccgaccgcac
     4321 caggtcgtgg tcatcggcaa gcacgatgcg aacaggctgc gatgcggcgg tgtttttcat
     4381 gggcccattt ataacctaca gcacggcgcg aatcgcaacg ccgcggcccg ggtaagtgcg
     4441 gatatgcagc cgcccgccgg ccagttcggt gcgctcgatc atcccgtaca ggccgacgtt
     4501 gtgctcgccg gcctgcccgt ccaggatccg gtgcttgtcg aacccgactc cgtcgtcgac
     4561 caccagcacg ccgattcggg cctggggcag gaagcgcagc cgcacggcga tgtgcgaggc
     4621 ctgcgcatgc cgaaccacgt tgttcagggc ctcctgcacc agccgaagcg cgaccgaagc
     4681 cgtttcgccg aggacggggg gttcctggcc gcgcgagctg acgctgaacg ccagttcggc
     4741 cggctcggcg atgcgctgca ccgccgtgtg cgtggccgcc accaacccca gcaggtcgag
     4801 ttgagccggt cgcagcgtaa acgacagtgt cttgagctgc ttgacggccg cctgagccat
     4861 gtccatggcc gtttccgtgt gttggtgcac cgcggcggga tcggctgcgc gcttggccgc
     4921 gtgcaggtgg atcaccatgc cggtgagcgt ctggcccagc tggtcatgga gctcgcgcga
     4981 aatcagtgtg cgttcccgct cctgcgccac caccagccgc gccgacagct ttttcaggcg
     5041 gcgataggct gcctcgagtt cgcgggccat gcgctcctgc tccagtaccc gccggcgttc
     5101 ggtcatcacg cgctcgatga tctgcggcag cgtccccagc ttgtccttgg tcaggtagtc
     5161 cttggcgcca cagcgcagca cgtgcaccgc cgcttcctcg ccgatggcgc gtgtgaccac
     5221 caccaggggc gtaccgcagc gccgcgccac cagaatctgc agtgccgcat agggggagaa
     5281 gcgcggcatg ttgaagtcgc acaggatgac gtcgaagtcc tgctgcaggg cgtcggtgaa
     5341 gccgctcgca tcctccaccc ggtgcagcac gtaacggcgc tgcgggtccg cacgctccag
     5401 cgcgatggcc atgagctcca catcgtctgg gctgtcttcc acgcacaaca acctgatcgt
     5461 ggcgggttcg gcgggatttg tcacggcatg ctcgggcgtt gggccccagt ttacctgttg
     5521 agatcgggtg aaaaccgccg catgcatagg ccggactgtt cctacacggc gatcagacgc
     5581 ccccatgcgg caggggcggg cgcatccgac accggcccct ggatcaggct cctagagtta
     5641 gcttgaaaca ggagaccctt atggaaacct tggacacaca gacattggcc attgtcgctg
     5701 tcgtgattct cgccctggtc gcggttggcg cctggatcat ctacaaccgc aagcagcagt
     5761 ccaaccggct gatgcaacga ttcggacccg aatacgggcg cgcggtcgac cagatgggaa
     5821 gccgcgccaa ggccgaagcg gaattgcgct cacgcgagaa gcgcgtcgag aaattcagga
     5881 tcgtgcccct ggcgccggcg gacgcggcga ggttcagcca ggactggaaa accctgcagg
     5941 gccgctttgt cgacaacccc aagggggtgc tcgtcgaggc cgacatgctg gtgcgcgagc
     6001 tgatggaaaa gcgcggctac ccgatgggtg acttcgaaag ccgggcggcc gacatttcgg
     6061 tggaccaccc cgccgtggtc gaccattacc gggcggcgca agccatcgcc atgcgcgacc
     6121 ggcgcgggga agccgatacc gaggagatgc gcaaggccgt ggtgcactac cgcgcgctct
     6181 tcgatgaatt gctcgaggtc aggcaggacg agcgggccgt aaccagggaa gcggaggtgc
     6241 attcatgaat accgtgcatg tggaagacag ggacaagacg cttacgaccg ccgacgtcgc
     6301 cgcggcggcg caacggccgc aggcgcagcc ggacttgaac cgcacaggca ccgtggatcg
     6361 atccaccgac ctgcggcagc agccggcggt gggcggggac gagcaactcg ccgcgctctt
     6421 cgcgaccgac atggccgacg actttcgctc gcgttgggac gcggtccaga tcgggttcgt
     6481 cgatgacccg cggcaggcgg tccggcaagc ggacgagctc gtggccgagg tgatgaagag
     6541 cctggcggag agtttctcgg ccgaacgcga caggttagaa agccagctcg agcaacagga
     6601 cagcgcctcc acggagaacc tgcgggtggc gctgcgccgc taccgctcct tcttccagcg
     6661 cctgctgtcg ctgtaagtcg gtggagagaa gggctggggg agggctgtca tgccggactc
     6721 gagccggcat ccatgacttc ctggattgc
//
LOCUS       DASWIY010000248         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2398930, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000248 DASWIY010000000
VERSION     DASWIY010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398930"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /gene="fliM"
                     /locus_tag="VGA59_09075"
     CDS             complement(<1..922)
                     /gene="fliM"
                     /locus_tag="VGA59_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEX9719870.1"
                     /translation="MNQQILSQDEVDALLQGITGESQKLEQEEAPTGGVRDYNLASQE
                     RIVRGRMPTMEIINERFARNIRIGLFNFIRKSPEISIGGIKVQKYSAFLREIVVPTNF
                     NIVSVRPLRGSGLIVCDPTLVFSVIDALFGGAGKYHTRIEGRDFSPTEQRIITRLVEV
                     ITLEYKKAWQGIYPLELEYQRSEMQPQFANIATPSEIVVATSFTLEIGDTTGTVHFCI
                     PYSTLEPIRDVLYSTIQGDSNEPDRRWVNLLKQQIQSAEVDLVAELATAPATVEQLLA
                     FKPGDFIELDLQAGIQAKVAGVPILDCHYGT"
     gene            complement(951..1580)
                     /locus_tag="VGA59_09080"
     CDS             complement(951..1580)
                     /locus_tag="VGA59_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX9719871.1"
                     /translation="MSVAAAETVEDGEAPPPPAKRGRKKLIVLATMALLVVAVLGGAA
                     AFVVKKRAAAAAALAGDEAPAQVSFTEARKAASKNPPTFLPLDPFIVNLADLDTERFA
                     QIGITLELSDAKFADELKAYMPAIRNGILMVMAHKTAKQLMGRAGKEALAREIMREAL
                     RPLGMELDEHELAAHADDEYEDEEAFAAPSQETDENPLRRVHFSSFIIQ"
BASE COUNT          269 a          553 c          622 g          358 t
ORIGIN      
        1 aggtgccgta gtggcaatcg agaatcggca cgccggccac cttggcctgg atgccggcct
       61 gcaaatcgag ctcgatgaag tcgcccggct tgaacgcgag cagttgctcc accgtggccg
      121 gcgcggtagc cagctcggcc accaggtcca cctcggccga ctggatctgc tgcttcagca
      181 ggttgaccca gcgccggtcg ggctcgttgc tgtcgccctg gatggtcgaa tacagcacgt
      241 cgcggatggg ctccagcgtc gagtacggaa tgcagaagtg gaccgtgccg gtggtgtcgc
      301 cgatctccag cgtgaacgac gtggccacca cgatctcgct cggcgtggcg atgttggcga
      361 actgcggctg catctccgag cgctggtatt ccagctccag tgggtagatg ccctgccagg
      421 cctttttgta ttcgagggtg atcacttcca ccaggcgcgt gatgatgcgt tgctcggtgg
      481 gcgagaagtc gcggccctcg atgcgcgtgt ggtacttgcc ggcgccgccg aacagcgcgt
      541 cgatcaccga gaacactagc gtcgggtcgc acacgatcag gcccgagccg cgcaagggcc
      601 gcacgctgac gatgttgaag ttggtgggca ccacgatctc gcgcaggaag gcgctgtact
      661 tctgcacctt gatgccgccg atggagattt ccgggctctt gcggatgaag ttgaacagcc
      721 cgatgcggat gttgcgggcg aagcgctcgt tgatgatctc catcgtgggc atgcgcccgc
      781 gaacgatccg ctcctggctg gcgaggttgt agtcgcgcac gccgccggtg ggcgcttcct
      841 cctgctccag cttctggctc tcgccggtga tgccttgcag cagggcatcg acttcgtctt
      901 gcgaaagtat ttgctggttc atcgtgcttc caccgggttg gtgacgcgac tcattgaatg
      961 atgaaactgg aaaagtgcac gcgccgcagc gggttctcgt ctgtctcctg cgagggcgcg
     1021 gcgaaggctt cctcgtcctc gtattcgtcg tcggcatggg ccgccagttc gtgctcgtcc
     1081 agctccatgc ccagcgggcg cagcgcctcg cgcatgattt cgcgggccag cgcttccttg
     1141 ccggcgcgtc ccatcagttg cttggcggtc ttgtgcgcca tcaccatcag gatgccgttg
     1201 cgtatggcgg gcatgtaggc cttgagttcg tcggcgaact tggcgtcgct gagttcgagg
     1261 gtgatgccga tctgcgcgaa gcgttcggtg tccaggtcgg ccaggttgac gatgaacggg
     1321 tccagcggca ggaaggtcgg cgggttcttg ctggccgcct tgcgcgcctc ggtaaagctg
     1381 acttgcgcgg gcgcctcgtc accggccaag gccgcggcgg ccgcggcgcg tttcttgacc
     1441 acgaacgcgg ccgcaccgcc gagcaccgcc accaccagca gggccatggt ggccaggacg
     1501 atcagcttct tccggccccg tttcgccggg ggcggaggtg cctcgccgtc ttcaacggtt
     1561 tcggcagcgg cgacggacat ggatcactcc ttcggttcac cacgcatgcg ggacgggagc
     1621 ccgcacgggt gtgtggcatg aagaaattgt cgaagtggcc ggccttgcag gatgcgggaa
     1681 taaggggggg aattcggggc cagtacgggc ggcgccgctt agcggttgag agtttttcgc
     1741 cgccgcacct cgctcccgtt cgccctgagg tatcgaaggg cctgctcggc ccggtggcag
     1801 gg
//
LOCUS       DASWIY010000249         3668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2399235, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000249 DASWIY010000000
VERSION     DASWIY010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2399235"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /gene="rpmG"
                     /locus_tag="VGA59_09085"
     CDS             <1..150
                     /gene="rpmG"
                     /locus_tag="VGA59_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX9719872.1"
                     /translation="EKIKLESTAGTGHFYTTSKNKKLMPEKMAIMKFDPKARKHVQYK
                     ETKLR"
     gene            complement(283..621)
                     /locus_tag="VGA59_09090"
     CDS             complement(283..621)
                     /locus_tag="VGA59_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719873.1"
                     /translation="MRKLMLAVFAVASLGAGVAFAQGGDAESQAGSAWRAPPDTAYGP
                     IPGTPEYYGNSGWPPADQRVYDYRSRSYVPYANRSAPAYPYFQPSRGDRDGDGVRNRR
                     DRYPDDPRRQ"
     gene            complement(820..2043)
                     /locus_tag="VGA59_09095"
     CDS             complement(820..2043)
                     /locus_tag="VGA59_09095"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX9719874.1"
                     /translation="MFLPDWAVLNAAVDWLANGLWNLSWWQVVLYTLVTTHITIASVT
                     IFLHRAQAHRAMDLHRIPSHFFRFWLWLSTGMVTKEWVAIHRKHHARCETPDDPHSPQ
                     TRGIDTVLWRGAELYRAEAKNQETIAKFGHGTPNDWIERNLYTRFSWQGVGLMLILNV
                     GLFGLAGLAVWAVQMAWIPITAAGVINGIGHYWGYRNFESPDASTNISPWGVIIGGEE
                     LHNNHHTYPTAAKFSVKKYEFDIGWVYITLMSRIGWATVKKVPPKLQLGAVKPVADEK
                     TLEALIANRYEVMAGYAREVRRVCKAEIAALKEKQADLSPLKAAKRWLHRDDDRVPPA
                     ARPQLARARAAHPVLDKMVTMREELRQMWLNTSESREQLAADLQSWCRRAEESGIAQL
                     QQFSMKLRAARVERK"
     gene            complement(2171..3490)
                     /locus_tag="VGA59_09100"
     CDS             complement(2171..3490)
                     /locus_tag="VGA59_09100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX9719875.1"
                     /translation="MHPKALLDACAELVKLTLKFEHPADAVVSRYFRDNRGLGPRERA
                     ALTETVYTVLRKKLLFDHLSPSGSGPKERRMAVLGFARSQDDQVRRAGASAEHGPRDF
                     LSSALTDQEKNWLDQCDSIKPSDLLERHRHNLPEWLVQPLKDQLGAQFWPLVESLNQP
                     APLDLRVNALTDKRPDVQRELKLAGIASSPTPYSPWGLRLDGKPALTKLDAFARGAVE
                     VQDEGSQLLALLLDAKRGEMVVDFCAGAGGKTLAIGATMRNTGRLYAFDTSAHRLDAL
                     KPRLARSKLSNVHPAAIAHERDERIKRLAGKIDRVLVDAPCSGLGTLRRNPDLKWRQS
                     PQLVQEMAVKQEAILRSASRLLKPGGRLVYATCSPLPQENEAIAQAFSLAHADFTPLP
                     IGEILTQLRVENAASLCSGGDAGLDFLRLWPHRHHTDGFFAAAWVKN"
BASE COUNT          635 a         1148 c         1210 g          675 t
ORIGIN      
        1 gaaaagatca agctggaatc caccgcgggt accggccatt tctacacgac cagcaagaac
       61 aagaaactca tgcccgagaa aatggcgatc atgaaattcg atcccaaggc tcgcaagcac
      121 gtgcagtaca aagagaccaa gctgaggtaa ttccgttggt ttccgctgcg ccagcagcac
      181 ccaagtaaaa gccccgcgac gcggggcttt ttttcacctt ggccgaaggc gtactgcggg
      241 gccttttacc tggtgccgct gcgcggcgcg tgctgctgcg ttttactgcc gccgcgggtc
      301 gtccggatag cggtcgcgtc gattgcgcac gccgtcgccg tcgcggtcac cgcggctggg
      361 ctggaagtac gggtacgcag gcgcagagcg gttggcatat ggcacgtaac tgcggctgcg
      421 atagtcatag actcgctggt cagccggcgg ccagccgctg ttgccgtaat actccggcgt
      481 tcccgggatc gggccatagg ccgtgtcagg cggcgctcgc caggccgagc cggcctgcga
      541 ttccgcgtcg ccgccctgcg cgaaagccac gcccgctccc agactggcaa ccgcgaacac
      601 tgcaagcatg agctttctca taggtaactc ctttggcgcg agcgctggtg gcccgcggat
      661 aacccattga acacccgccc catataggtt gcatgtctac cgtgtgccga aagcatgtcg
      721 gacggttacc agcggtagcg taaggaatac gccctcggcg aaggtgaaaa aaagccccgc
      781 ggagcggggc ttttccttgg ccgctggctg tgcagcgatt tacttgcgct ccacgcgagc
      841 ggcgcgcagc ttcatcgaga actgctgcag ctgggcgatg ccgctttcct cggcgcggcg
      901 gcaccaggat tgcaggtcgg cagccagctg ctcgcgcgac tcggacgtgt tgagccacat
      961 ctgccgcagt tcctcgcgca tggtgaccat cttgtcgagc accgggtgag cggcgcgagc
     1021 ccgggccagc tgcggccggg ccgcgggcgg caccctgtcg tcgtcgcggt gcagccagcg
     1081 ctttgcggcc ttcagcggcg acaggtcggc ctgcttctcc ttcagcgcgg cgatctctgc
     1141 cttgcagaca cggcgcactt cgcgcgcgta gccggccatg acctcgtagc ggttggcgat
     1201 cagcgcttcc agcgtctttt cgtccgccac aggcttcacg gcgcccagtt gcagcttggg
     1261 cggcaccttc ttgacggtgg cccagccgat cctgctcatc agggtgatgt aaacccagcc
     1321 aatgtcgaac tcgtacttct tgaccgagaa cttggccgca gtcgggtagg tgtggtggtt
     1381 gttgtggagt tcctcgccgc cgatgatcac gccccagggc gaaatgttgg tgctggcgtc
     1441 aggcgattcg aaattgcgat agccccagta atggccgatc ccattgatca cgccggcggc
     1501 ggtgatcggg atccaggcca tctgtacggc ccacacggcc agcccggcaa ggccgaacaa
     1561 gcccacgttc aggatcagca tcaggccaac gccctgccag ctgaagcggg tgtacaggtt
     1621 gcgttcgatc cagtcgttgg gggtcccgtg gccgaatttc gcaatggttt cctggttctt
     1681 ggcttccgca cggtagagct cagcgccgcg ccacagcacg gtgtcgatgc cacgcgtctg
     1741 cgggctgtgc gggtcgtcgg gcgtctcgca cctggcgtgg tgcttgcggt ggatggcgac
     1801 ccattccttg gtcaccatac cggtgctcag ccagagccag aaccggaaaa agtgcgacgg
     1861 aatgcggtgc aggtccatgg cacggtgcgc ctgggcacgg tgcaggaaga tggtgacact
     1921 ggcgatggtg atgtgggtgg tcaccagggt gtacagaacg acctgccacc aggacaggtt
     1981 ccacaggccg ttggcgagcc agtcaacggc cgcgttcaat acggcccaat cgggaagaaa
     2041 cataagcggg gagtactctt tcgaaaaaca agctgggcca ttgggcccca caatgagaga
     2101 gcgattctag cgggggaggt tcaatcgcgc gcgggattta ccacaaaacc ggccaaatcg
     2161 ccgccctgcg ttaatttttt acccaggcgg cggcaaaaaa cccatccgtg tggtgccggt
     2221 gcggccacaa gcgcaggaaa tccaggccag cgtccccgcc gctgcacagg ctggcggcgt
     2281 tctcgaccct cagctgtgtc aggatttcgc cgatcggcag gggcgtgaag tcggcgtgtg
     2341 cgaggctgaa cgcttgcgcg atagcctcgt tttcctgggg cagcgggctg catgtcgcat
     2401 agacgagccg cccgccgggt ttgagcagcc gcgacgcgct gcgcaggatc gcctcctgct
     2461 tgacggccat ctcctgcacc aactgcggcg actggcgcca cttgaggtcc gggttgcgcc
     2521 gcagcgtgcc caggccggag catggcgcgt cgacgaggac acggtcgatc tttcccgcca
     2581 gccgcttgat gcgctcgtcc cgctcgtgcg cgatcgccgc cgggtggacg ttggacaact
     2641 tgctgcgggc cagccgcggc ttgagcgcat cgagccgatg cgccgaggta tcgaacgcat
     2701 aaagccggcc ggtgttgcgc atggtggcgc cgatggccag agtcttgccg ccggctccgg
     2761 cgcagaagtc caccaccatt tcgccgcgct tggcatccag cagcagcgcc agcagctgcg
     2821 agccttcgtc ctgcacctcc accgcccctc gcgcgaacgc gtccagcttc gtcagcgccg
     2881 gcttgccgtc caggcgcagg ccccagggcg aataaggggt cgggctcgag gcgataccgg
     2941 ccagcttcag ttcacgttgg acgtcggggc gcttgtcggt cagggcgttg acgcgcaggt
     3001 ccagcggcgc cggctggttg aggctctcca ccaggggcca gaactgggcg cccaactggt
     3061 ctttgagggg ctgcaccagc cattccggca ggttgtggcg atggcgctcc agcaggtcgc
     3121 tcggtttgat cgagtcgcac tggtccagcc agttcttttc ctggtccgtg agcgcgctgc
     3181 tgaggaagtc acgcgggccg tgctcggcgc ttgcgccggc gcgtctgact tgatcgtcct
     3241 gactacgcgc aaagcccagg acggccatcc ttcgctcttt cggtccgctg cccgacggcg
     3301 acaaatggtc gaacaacagc tttttgcgca ggacggtgta gacggtctcg gtcaatgccg
     3361 cgcgctcgcg cgggccgagc ccgcggttgt cgcggaagta gcgcgacacc acggcatcgg
     3421 ccggatgttc gaacttgagg gtgagcttga cgagttcggc gcaggcgtcg agcagggctt
     3481 tgggatgcat ggagcgattg tcccattgag ccttgccacg gctgtgcagc cgcgatagcg
     3541 ctgtcatgcc gggcttgacc cgaatacgag gggacttccc accttccttg aacggcgtca
     3601 aagcgccaag attgatgttg ggaaaaacgt caatctgaac ggaaaggaga agtcccatga
     3661 gcgagatt
//
LOCUS       DASWIY010000250        20845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_154316, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000250 DASWIY010000000
VERSION     DASWIY010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 20845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20845
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154316"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VGA59_09105"
     CDS             <1..524
                     /locus_tag="VGA59_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9719876.1"
                     /translation="IMTNVARGYASMGKPAEALVYLERARKVQPRAPAVRSLEVILLS
                     RTGQEARALELARQAIADKIYDYDLANASFILGWRSGDYALAAQAMRLRMIGWPATRV
                     AGYVQLGEMYAHAAKDPDQALAAFKQALALAPEAQRPALLQRIPPAYRARLGYPQAAP
                     QAPGTIQTSASQG"
     gene            complement(500..1417)
                     /locus_tag="VGA59_09110"
     CDS             complement(500..1417)
                     /locus_tag="VGA59_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX9719877.1"
                     /translation="MTQLLNGVTPLPHLGIIRVEGDDAAAFLHAQLTQDFAMLGMSEA
                     RLAAFCTAKGRMQASFIGFKRGHADILLVVSRDVLAPTLKRLSMFVLRAKAKLRDATA
                     EFELYGLAGESAESMAPGPQEPWTKADIGNASVVRLYPADGQARSLWVAPAGEPAPAG
                     PALSLERWLWGEVRSGVATITTPIVEALVPQMLNYESVGGVNFKKGCYPGQEVVARSQ
                     FRGTLKRRAYPAHSPSALQAGQEVFHESDASQPCGLVAQAAAAPDGGWDAIVSIQIAA
                     AQDGRLTAGGPEGAGLSLQPLPYPWLADV"
     gene            1507..2502
                     /gene="mltG"
                     /locus_tag="VGA59_09115"
     CDS             1507..2502
                     /gene="mltG"
                     /locus_tag="VGA59_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX9719878.1"
                     /translation="MRRFLLTVFLLASLAALGAGGWALWWVYQPLRLSTPTVDLSIEP
                     GTLPRGVAQAVRDAGVDVNPELLYWWFRLSGQDRLIKAGSYELETGITPHRLLAKVAR
                     GEESLRAVTLVEGWNFRQVRAALAGENMLKPDTREMADDALMSLLGRPGVHPEGRLFP
                     DTYTFAKGSSDVAVLKRAMRAMDKRLAFAWAQRSPAAVVKTPDEALILASIIEKETGK
                     PADRALIAAVFSNRLRVGMPLQTDPTVIYGMGAAFDGNLKKKDLQTDTPWNTYTRTGL
                     PPTPIAMPGKASLLAAVQPAPGNALYFVSRGDGSSQFSANLDEHNRAVNKYQRGQ"
     gene            2499..3146
                     /gene="tmk"
                     /locus_tag="VGA59_09120"
     CDS             2499..3146
                     /gene="tmk"
                     /locus_tag="VGA59_09120"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX9719879.1"
                     /translation="MTKGGLFISFEGIDGAGKSSHVQALADAFRGQGRIVTVTREPGG
                     TPLAEQLRALVLNDAMDPLTEALLMFAARRDHLLQVIEPALARGEVVLCDRFTDATFA
                     YQGHGRGFDRQVLAGLERWVQSVPALPAGTLRQPHLTLWFDLAPEIAASRLAGARAPD
                     KFESEPVEFFRRVAAGYAQRLAAQPSRFARIAADQPREAVWNDVLAALQRQGWLA"
     gene            3143..4138
                     /locus_tag="VGA59_09125"
     CDS             3143..4138
                     /locus_tag="VGA59_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX9719880.1"
                     /translation="MKPPPPWIAAQVRQLLAQRGHAWLLQGPSGLGQYSLALELARAW
                     LCDQPAAQGACGQCASCHAVDVRAHTDLCVLMPETIMLELGWPLGEKAQQDLDEKKRK
                     PSKEIRVDAMRDAVEFAQRTSGRGRGKVALVYPAERMNHVTANTLLKTLEEPAGDLRF
                     VLASEAAHQLLPTIRSRCLGHTMTWPEESAALAWLQAQGIKPADAPALLRAAGGRPED
                     ALSFAQAGRDAKVWAVLPKALARGDAAALADWTPAEAVSALQKICHDLMAVKTGGAPR
                     FFQASDLPAAASFAALTAWARELANTARTVEHPFNAGLMLETLVSQARSALNSRP"
     gene            4140..4502
                     /locus_tag="VGA59_09130"
     CDS             4140..4502
                     /locus_tag="VGA59_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9719881.1"
                     /translation="MSTAAPHSPRPSVIQLAIKEKAALYAAYIPLFADGGVFIPTTRD
                     YKLGDDVYVLLSLPDDPQRYPVAGKVAWVTPARAAANRTQGVGIRFPSDEKSRLLKIR
                     IEEILGGHLASERPTQTI"
     gene            4566..5372
                     /locus_tag="VGA59_09135"
     CDS             4566..5372
                     /locus_tag="VGA59_09135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX9719882.1"
                     /translation="MFVDSHCHLAFPELASQLPEIRQAMAQAQVDRALCICTTLEEFD
                     AVHALAMQYDNFWATVGVHPDSEDVTEPGIQDLVRRCGLPRVVAIGETGLDYYQMDER
                     KGGRTVADMEWQRERFRTHIRAARQAGQPLVIHTRAASADTLAILKEEGEDGSAGSAG
                     GVFHCFTETAEVARAALDRGFYISFSGILTFKSAQELRDVAAFVPLDRLLIETDSPYL
                     APVPYRGKINNPSYVPYVARQLAELRKMPVDEVARLTSANFEHLFRVALS"
     gene            5369..6028
                     /locus_tag="VGA59_09140"
     CDS             5369..6028
                     /locus_tag="VGA59_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX9719883.1"
                     /translation="MINYFKNIVYLIVITGFSIAYAGSYEDFFIAIKQDNPAAVRTLL
                     QRGFDADTLDPKGLSGLYLALRDGSLKAAQTLIDWPKTNIEIRTAQDESPLMIAALKG
                     HLEIVKKLIARDADINKTGWAPLHYAATGGHPAVIELLLEHHAFIDAESPNGTTSLMM
                     AAHYGSQAAVKLLLEAGADPTMRNQLGLSAIDFANRAGRQDAADMVAAYVRKAQPKGK
                     W"
     gene            6298..6654
                     /locus_tag="VGA59_09145"
     CDS             6298..6654
                     /locus_tag="VGA59_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719884.1"
                     /translation="MQSMSSLFIVSALVLAGCASRPPGEAGTHLVYRDASGKPTMQID
                     YPSVDFCRRVEAIARRDSRCEAKSASSELQASATLRYNPGNMLVEGHYPDVAACQRAN
                     AKMARGVELAKPCTAK"
     gene            6959..7303
                     /locus_tag="VGA59_09150"
     CDS             6959..7303
                     /locus_tag="VGA59_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719885.1"
                     /translation="MRWLKPNLRSSIYQLLGNPVDPSESMLETGTEDIRESMLAVLGE
                     GGPKHFPQITRRIRYANDIQALWYLRGDLMAALAAMHGEVTAREKVASVTQMFKGLLP
                     GALNSRPSPLVG"
     gene            complement(7316..8230)
                     /locus_tag="VGA59_09155"
     CDS             complement(7316..8230)
                     /locus_tag="VGA59_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX9719886.1"
                     /translation="MTKHIYVYSPSSAVRSKVVIRRGVARLQALGYEVELDEAVFATS
                     MRFAGDDETRLAAIHRAAASGADVALITRGGYGLTRLLPHIRYKAVAKAIDKGMRFVG
                     VSDFTAFQTAMLAETGAVTWAGPALGEDFGVEGKPDDIMQACFEDLVGGQGEGTGWQL
                     PRAEAAVRPIELKGELWGGNLAVLATLVGTAWMPRIKGGILFLEDVHEHPYRVERMLT
                     QLLYAGVLARQKAIVLGHFTNYKPVPHDKGYRLQTVIDWLREQAKVPVFTNLPFGHVP
                     TKVSLPVGAKVHLAVEGRDAFILWGHRH"
     gene            complement(8227..9582)
                     /gene="tadA"
                     /locus_tag="VGA59_09160"
     CDS             complement(8227..9582)
                     /gene="tadA"
                     /locus_tag="VGA59_09160"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX9719887.1"
                     /translation="MGLALQQARLAGAAGEVPVGAVVVRDGQVIGVGRNAPVAGNDPT
                     AHAEIVALRQAAQASGNYRLDGCDLYVTLEPCAMCSGAMLHARLRRVVFGAADPKTGA
                     AGSVVDLFAQPRLNHQTRVQGGVLADACGELLGEFFRTRRLDARLVADPLRDDALRTP
                     DERFEELPGYPWQPRYLSDLPSLAGLRMHYLDEGPADAPLTWLCLHGNPAWSYLYRKM
                     IPVFLAAGDRVVAPDMIGFGRSDKPKREAVHSFGWHRQVLLELVERLDLRNVVLVVQD
                     WGGLLGLTLPMAAPRCYLGLLAMNTALGTGDTALSPGFLAWREMCAKNPDFDVARLFA
                     RGNAQMSPEECLAYNAPFPDRGHRAALRAFPAMVPDRPDADGAAISRDARMFMSSEWT
                     GRTMMVIGAKDPVLGLPAMQALRQSIRGCPEPMVIEEAGHFVQEHGEPIARAAVGYFR
                     P"
     gene            complement(9597..9824)
                     /locus_tag="VGA59_09165"
     CDS             complement(9597..9824)
                     /locus_tag="VGA59_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719888.1"
                     /translation="MRAVFGILSLLVVVAIVGLLARKQLGATAPVAAPASTPASGSMA
                     APAGTPQQQVRQFQQAVQGAMQQARPMPEDK"
     gene            complement(9837..10529)
                     /locus_tag="VGA59_09170"
     CDS             complement(9837..10529)
                     /locus_tag="VGA59_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX9719889.1"
                     /translation="MYQPPQFRSTDRAIAARLMREHPFASLISNDDAGLPFVTHLPLH
                     LEDREASGASERPGFVLLGHCARSNPHWRYLQQRPTAVVTFLGPHAYQSPRIYPDLAR
                     VPSWNYLAVHCTVTATLVDEPLVKDRLLKKLIGDHEPPYAQQWRSLGEDYARKMLAGI
                     MAFELQVTALECKLKLNQHRPESHAALRDGYARGNEQERELAQWMDVLGIGEPVHSPA
                     AGQAVLGRQEEK"
     gene            10660..12177
                     /locus_tag="VGA59_09175"
     CDS             10660..12177
                     /locus_tag="VGA59_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9719890.1"
                     /translation="MNSKELLEKTGISRATLNNYISWGIVPKPEVLPPEPQDGAAPRI
                     GYFPDEVVQRIEDIQRLKREGWSITRIAEHFNAAAGDPSNEAAGTAMASPVPKRGAMP
                     NVSIDEITNPAYIVNHRLEVTWFNAAAGAGVLRDLPQSPEDAASRNVFRYLLHSPSTD
                     ANSREQVLRFHLGLAKQHGASFSLLCREVPREEVGWLKRLHDEAKQPEPGVVSHALLP
                     GGGAGLASSVCVYAVQFREGILILYVPGGRASQVPSQPLVQRELVTPEAGGKVQPMLS
                     RAAVLAADLQHPHRIWSELPPEEYFELINQIWLTVEPIFKRHNGTHGKHHRDGVVCYF
                     FPQPDGSHVWNALVAALEMRDAMRRVSKAWQLRKGWTTELYMNTGIDEGPQWLGTLKS
                     PPKAEFTMLGDTLNHAVRISDFASFGSIWVTKNLIGKLTTDEKQRLKYGVRRRSNDGR
                     DIFVASVFSNVDSLTDAAAGPAEKLKGIARLPITEVVEIAVPDRRVDLAAEHTLA"
     gene            12189..12587
                     /locus_tag="VGA59_09180"
     CDS             12189..12587
                     /locus_tag="VGA59_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719891.1"
                     /translation="MGWFKTHIVSTISALFSSGLSSAPAELEMEVGIEEIRAAMLDLI
                     TDVDDKKFMPVVRRIRYATDVLSLWYLRGDLMAALAARYGEEQARESLKPITGMFTNM
                     LPPGLRSRVSPLASASRDWQASDRNSDLRK"
     gene            complement(12639..14252)
                     /gene="guaA"
                     /locus_tag="VGA59_09185"
     CDS             complement(12639..14252)
                     /gene="guaA"
                     /locus_tag="VGA59_09185"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX9719892.1"
                     /translation="MQHDKILILDFGSQVTQLIARRVREAHVFCEVYPCDVTGEWVRE
                     YAADGRLKGVILSGSHASVYEDTTDKAPQAVFELGVPVLGICYGMQTMAHQLGGKVEG
                     GHKREFGFASVRARGHTRLLKDIADFNTPEGHGMLDVWMSHGDKVTELPPGFKLMAST
                     ESCPIAGMADEARGFYGLQFHPEVTHTRQGRAMLERFVLDICGARSDWVMHDHVAEAV
                     DLIRRQVGDEEVILGLSGGVDSSVAAALIHRAIGEQLTCVFVDHGLLRLNEGDMVMEM
                     FAGKLHAKVIRVDASELFLGKLAGVGDPEAKRKIIGREFVEVFKAEAAKIKQTHARHV
                     AWLAQGTIYPDVIESGGAKNKKAVSIKSHHNVGGLPEQLGLKLLEPLRDLFKDEVREL
                     GVALGLPPEMVYRHPFPGPGLGVRILGEVKKEYADLLRRADAIFIEELRDCEDKATGK
                     SWYDLTSQAFAVFLPVKSVGVMGDGRTYDYVVALRAVQTSDFMTADWAELPYPLLKKV
                     SSRIINEVRGINRVTYDVSSKPPATIEWE"
     gene            complement(14256..14672)
                     /locus_tag="VGA59_09190"
     CDS             complement(14256..14672)
                     /locus_tag="VGA59_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9719893.1"
                     /translation="MLVVDASAFASWAFPDEHGDKVSQSLVRIMTEEESASSAAIFPL
                     EALHAAGRALARGRFTPTVHQHVLRLLSRLPVALAPLRLSPLEFWSWSQSLQLTAYDA
                     AYLKLAVDLKAPLVTMDRNLQRACLQLDHPVITDLP"
     gene            complement(14659..14976)
                     /locus_tag="VGA59_09195"
     CDS             complement(14659..14976)
                     /locus_tag="VGA59_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9719894.1"
                     /translation="MQTVPIHQAKSQLSELIRAVEQGEEVVLTRHGKRVIRLIKEPES
                     VQPSVEARRQAIMAELEALRSKVGSGQPGFSELWEEHKRELHERPDPWIDAAQAKGGN
                     AGR"
     gene            complement(15079..16548)
                     /gene="guaB"
                     /locus_tag="VGA59_09200"
     CDS             complement(15079..16548)
                     /gene="guaB"
                     /locus_tag="VGA59_09200"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX9719895.1"
                     /translation="MRLLGKALTFDDVLLVPAFSQVLPKDTSLATRLSRNITLNLPLV
                     SAAMDTVTEARLAIAIAQEGGMGIVHKNLTAKQQAAEVSRVKRYESGVLRDPVVITPG
                     HTVRQVIDMQVQLGISGFPVIDGGKVVGIVTGRDLRFEERYDVPVRDIMTPREKLITV
                     KEGTSAAEAKALLNKYKLERLLVINDAFELKGLITVKDITKQTSFPNAARDASGRLRV
                     GAAVGVGVGTEERVEALVKAGVDAIVVDTAHGHSKGVIERVRWVKKNYPQVDVIGGNI
                     ATGAAARALVEAGADAVKVGIGPGSICTTRIVAGVGVPQVTAIDNVATALQGSGVPLI
                     ADGGVRYSGDISKAIAAGASTVMMGSMFAGTEEAPGEIVLFQGRSYKSYRGMGSIGAM
                     EQGSADRYFQEATTGNPNADKLVPEGIEGRVPYKGSLVAIVYQMSGGVRASMGYCGCA
                     TIEEMKSKAEFVEITVAGIRESHVHDVQITKEAPNYRAD"
     gene            16690..17196
                     /locus_tag="VGA59_09205"
     CDS             16690..17196
                     /locus_tag="VGA59_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX9719896.1"
                     /translation="MLLLRIFVLGLACALPAISLAQWQWIDKDGRRVFSDQSPPADIP
                     AKNIIRQPGVKSPSPEPVAVADPQPAKPAAPKLSGKDQALEQKKKQAEAAEAEKKKSD
                     EERIAKARADNCERSKRAKAGFDSGVRIARINAKGEREYLDDAARAAETKRLEGIIAG
                     DCKPAGAQ"
     gene            complement(17211..17534)
                     /locus_tag="VGA59_09210"
     CDS             complement(17211..17534)
                     /locus_tag="VGA59_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEX9719897.1"
                     /translation="MARPEIRAVVVYSPAPREVHEWPLVLAPGSTVLGALLACGLNEA
                     FPDLDLRSAAAGVWGRKAGLEQALRDGDRVEVYRPLKVDPKLARRERFRQQGVRSAGL
                     FARRT"
     gene            complement(17521..17964)
                     /locus_tag="VGA59_09215"
     CDS             complement(17521..17964)
                     /locus_tag="VGA59_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX9719898.1"
                     /translation="MKTVHKSVLIWYSAEEMFALVTDVDRYPEFLPWCDHASVVPEGE
                     GMKAEIGIAFGGIHQTFRTHNEHVPGREVSMKLIEGPFSRLDGQWKFTPLGTPEQRAC
                     KVELELHYGFDNAALASLVGPVFGKIASSMVEAFVKRAEQVYGST"
     gene            18023..18496
                     /gene="smpB"
                     /locus_tag="VGA59_09220"
     CDS             18023..18496
                     /gene="smpB"
                     /locus_tag="VGA59_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX9719899.1"
                     /translation="MAKKPETSSRIADNKKAAYNYFFEEKFEAGMVLQGWEVKALREG
                     KVQLTDGYVVVKNGELFVIGCQINPLKTASTHVNPDAVRTKKLLMHKDEIRRLVGKVE
                     QKGYTLVPLNLHWKEGKVKCEIALAKGKAEHDKRDTIKDREGKREVERAMKSRNR"
     gene            18690..19211
                     /locus_tag="VGA59_09225"
     CDS             18690..19211
                     /locus_tag="VGA59_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9719900.1"
                     /translation="MNRILLVEQLPGLRRYARALTGDAWAADDLVQDTLERACIKWQL
                     WVAGTDLRAWLFTLMHNLYLNQRRAARVALNSVSIDDVEAELRAPAAGIDGPLDLDRC
                     LQRLPADQRAVLLLVALEDMSYEDAAKVLAIPLGTVMSRLSRARSRLRELMEGGKPAA
                     WTAAPAPGLRRMK"
     gene            19222..20043
                     /locus_tag="VGA59_09230"
     CDS             19222..20043
                     /locus_tag="VGA59_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX9719901.1"
                     /translation="MDPRQDDPAPIPEDDLHALVDARLAPEDAAALRRRLAADPQAKA
                     TAAAWQAQRDELRGRYAQMEDLPLPASLVATAQRAERALAQTSQWWRWGGMAAALLLA
                     FGMGWLASSQWRTDHGDTALARSEPARHFARQAAMAYAVFQPEVRHPVEVTAAQQDHL
                     VQWLSKRLGRQLKVPVLTAQGYELVGGRLLPGDDGGARAQFMYQNSAGQRITLYLGAV
                     KSDVGGAARETAFRFLQEGPVPGFYWVDQGFGYAISGTLPRPALQELAKVVYGQL"
     gene            complement(20051..>20845)
                     /locus_tag="VGA59_09235"
     CDS             complement(20051..>20845)
                     /locus_tag="VGA59_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9719902.1"
                     /translation="RQLEHARSFEITFWRSFFTVASLMVILPLFQGRQVFARIRDGGP
                     ALWISGMCWSVMFTAFMVALTLTSVANVLVTMSVGPFLTALMARVFIGHRVPVRTWLA
                     ILVAGSGIAYMYAAQVSGGQLAGTLVALCVPLAGAVNWTVVQRSNARGLNVDLVPAVL
                     VGAIISTVTMLPPAMPLQASGHDIGLLAVLGLFQLAIPCSLSVVCARVLKAPEIALLA
                     SLEVIFGILLAWVGAGEVPSHTVIAGGALVLGALVVNELVGWKQRA"
BASE COUNT         3713 a         6977 c         6690 g         3465 t
ORIGIN      
        1 cgatcatgac gaacgtcgcg cgcggctatg ccagcatggg caagcctgcc gaggcactgg
       61 tgtacctgga gcgggcccgg aaggtccagc cgcgcgcccc ggccgtgcgt tcgctggagg
      121 tgatcctgct cagccgcacc ggccaggagg ctcgggcgct cgagctggcg cgccaggcca
      181 tcgccgacaa gatttacgat tacgacctgg ccaatgccag tttcatcctg ggatggcgtt
      241 cgggcgacta tgcgctggcg gcgcaagcga tgcggctgcg catgatcggc tggcccgcga
      301 cgcgggttgc gggctacgtc cagctagggg agatgtacgc gcatgccgcc aaggatccgg
      361 accaggccct ggcagccttc aaacaggccc tggcattggc cccggaggcg cagcgaccgg
      421 ccctgttgca gcgcataccg cccgcctacc gggcgcggct cggctaccct caggcggcgc
      481 cccaagcgcc tggaacgatt cagacgtcgg ccagccaggg atagggcagg ggctgcagcg
      541 acaaaccggc accttccggc ccgcctgcgg tcaaccggcc atcctgcgcg gccgcgatct
      601 ggatcgacac gatcgcatcc cagccgccgt cgggcgcggc cgcggcttgc gcaacgaggc
      661 cgcagggctg gctggcgtcg gactcatgaa aaacctcctg gccggcctgc aaggcggacg
      721 gcgagtgcgc gggataggcg cgccgcttga gtgttcccct gaactggctg cgcgccacca
      781 cctcctggcc cggatagcag cccttcttga aattgacgcc accgaccgat tcgtagttga
      841 gcatctgcgg caccaacgcc tccacgatgg gcgtcgtgat ggtggcgacg ccgctgcgaa
      901 cctcgcccca caaccaccgc tcgaggctca gcgcgggccc ggccggggcg ggctcaccgg
      961 cgggggccac ccacaggctg cgggcctggc cgtccgccgg ataaaggcgc accacgctgg
     1021 cgttgccgat gtcggctttg gtccagggtt cctgagggcc gggcgccatg ctctcggcag
     1081 actctcctgc gaggccgtac agctcgaatt cggccgttgc gtcgcgcagc ttggccttgg
     1141 cgcgcaggac gaacatcgac agccgcttga gcgtaggcgc gaggacgtcc cggctcacga
     1201 ccagcaggat gtcggcatgg ccgcgcttga atccgatgaa gctggcctgc atgcggcctt
     1261 tggccgtgca aaaggcggcc aggcgcgcct cggacatgcc cagcatcgcg aagtcctgcg
     1321 tcagctgggc gtgcagaaaa gcggcggcat cgtcgccttc cacgcgaatg atgcccagat
     1381 ggggcagggg cgttacaccg ttcaggagtt gagtcatcga atcctcacgt ttgccgccga
     1441 agcggctgca tccattgaat tatcatcgtc tgtccaattt actccatggc ataggggcag
     1501 cgatcggtgc ggcgtttcct gttgacggtc ttcctgctgg cttcgctggc cgccctgggc
     1561 gcaggcggtt gggccctgtg gtgggtctat cagccgctgc gcctgtccac gcccacggtg
     1621 gacttgtcga tcgaacccgg gaccctgccg cgcggtgtcg cgcaggccgt gcgcgatgcc
     1681 ggcgtcgatg tgaatcccga attactgtac tggtggttcc gcttgtccgg ccaggaccgg
     1741 ctgatcaagg ccggcagcta cgagctcgag accggcatca cgccgcatcg cctgctggcc
     1801 aaagtcgcgc ggggcgagga aagcctgcgt gccgtgacgc tggtcgaagg ctggaacttc
     1861 cggcaggtcc gcgccgcgct ggcgggcgag aacatgctca aacccgacac tcgcgaaatg
     1921 gccgacgacg cgctcatgtc cctgctcggc cggccgggcg tacacccgga agggcgcctt
     1981 ttccccgaca cctacacctt tgccaaaggc agcagcgatg tggccgtgct caaacgcgcg
     2041 atgcgggcca tggacaagcg cctggccttc gcgtgggccc agcgttcgcc ggcagccgtg
     2101 gtcaagacgc cggacgaggc gctgatcctg gccagcatca tcgaaaagga gaccggcaag
     2161 ccggcggacc gggcgctgat cgcggccgtg ttcagcaacc ggttgcgcgt gggcatgccg
     2221 ctacagaccg accccacggt gatctatggc atgggcgcgg ccttcgacgg caacctgaaa
     2281 aagaaggatc tccagaccga tacgccctgg aatacctaca cccgcaccgg cctgccgccg
     2341 accccgatcg cgatgccggg caaggcttcc ctgctggcgg ccgtgcagcc ggcccccggc
     2401 aacgccttgt acttcgtttc ccgcggcgac ggcagcagcc agttcagcgc caacctggac
     2461 gagcacaacc gcgccgtcaa caaataccag cgtgggcaat gacgaaaggc ggcctcttca
     2521 tcagtttcga gggcatcgat ggcgccggga agtccagcca cgtccaggcg ctggcggatg
     2581 catttcgcgg ccagggcagg atcgtgacgg tcacacgtga gccgggcggg acgcccttgg
     2641 ccgagcaact ccgggcgctg gtgctcaacg acgcgatgga cccgctgacc gaggcgctgt
     2701 tgatgttcgc ggcgcggcgg gaccacctgt tgcaggtcat cgaacccgcc ctggcgcgcg
     2761 gtgaagtggt gctgtgcgac cggttcaccg acgcgacttt cgcctaccag ggccatgggc
     2821 gcggtttcga tcgccaggtc ctggccggcc tggagcggtg ggtgcagtcg gttcccgcgc
     2881 tgccggccgg cacgctgcgg caaccccacc tgacgctgtg gttcgacctg gcgcccgaga
     2941 ttgcggcgag ccggctggcc ggcgcccgcg cgcccgacaa gttcgaatcc gagccggtgg
     3001 agttcttccg gcgggtggcc gccggctacg cgcagcggct ggcggcgcag ccgtcccggt
     3061 ttgcacgcat cgccgcggac cagccgcgcg aggcggtatg gaacgatgta ctggccgcac
     3121 tgcagcggca aggctggctc gcatgaaacc accaccgccc tggatcgccg cgcaagtgcg
     3181 gcaattgctg gcccagcgcg gccatgcctg gctgctgcaa gggccctcgg gcctggggca
     3241 atacagcctg gcgctggaac tggcacgcgc ctggttgtgc gaccagccgg ccgcccaggg
     3301 cgcgtgcggg cagtgtgcgt cctgccacgc ggtcgacgtt cgcgcgcaca cggacctgtg
     3361 cgtgctcatg cccgaaacca tcatgctgga gctgggctgg ccgttgggcg aaaaagcgca
     3421 gcaggacctg gacgagaaaa aacgcaagcc gagcaaggaa atccgggtcg atgcgatgcg
     3481 cgacgccgtc gagttcgccc agcgaaccag cggacgcggg cgcggcaagg tcgcgctcgt
     3541 gtatccggcc gaacgcatga accatgtcac ggcgaacacc ctgctcaaga ccttggagga
     3601 gccggccggc gacctgcgct tcgtgctcgc cagcgaggcg gcgcaccagt tgctgcccac
     3661 cattcgcagc cgctgcctgg gccacaccat gacttggccc gaagaaagcg cggccctggc
     3721 ctggctgcag gcgcaaggaa tcaagcccgc cgatgccccg gccctgttgc gcgccgccgg
     3781 cgggcggccc gaagacgcgc tgtcgtttgc gcaagccggc cgcgacgcca aggtgtgggc
     3841 cgtgctgccg aaggcgctgg cccggggcga cgccgccgcg ctcgccgact ggacgccggc
     3901 cgaggccgtg agcgccctgc agaaaatctg ccacgacctg atggcggtga aaacgggagg
     3961 ggcgccccgg ttttttcagg cctcggacct gccggccgcg gcctcgttcg cggccttgac
     4021 ggcctgggcg cgtgaactgg cgaatacggc gcggacggtc gaacacccct tcaatgcggg
     4081 gctgatgctc gaaacgcttg tgagccaggc ccgctcggca ctaaactcga ggccttgata
     4141 tgagcaccgc cgcgccgcac tccccccgtc ccagcgtcat ccagcttgca atcaaggaaa
     4201 aggccgccct gtacgcagcc tacattccgc tgttcgccga tgggggcgtt ttcatcccga
     4261 ccacccgcga ttacaagctc ggcgacgacg tctacgttct gctgtcgctg ccggacgacc
     4321 cgcagcgcta cccggtcgcc ggcaaggtgg cgtgggtgac gccggcgcgc gccgccgcca
     4381 accgcaccca gggcgtcggt atacgcttcc catcggacga aaaatcgcgg ttgctgaaga
     4441 tcaggatcga agagattctg ggcggccacc tggcgtccga gcggcccacc caaaccatct
     4501 gagacgctac aatttttgta gctttccgtg gcgcggccgc gagcccagcc attgcactgc
     4561 ccaccatgtt tgtcgactcg cactgccacc tcgcttttcc cgaactcgct tcgcagctgc
     4621 ctgaaatccg gcaggccatg gcgcaagcgc aggtggaccg cgcgctgtgc atttgcacca
     4681 cgctggaaga gttcgacgcc gtccacgccc tggccatgca gtacgacaat ttctgggcca
     4741 ccgtgggcgt gcacccggac agcgaggacg tgaccgaacc gggcatccag gacctggtgc
     4801 ggcggtgcgg cctgccgcgc gtcgtcgcga tcggcgaaac cgggctggac tattaccaga
     4861 tggacgagcg caagggcggc cggacggtgg ccgacatgga atggcagcgc gagcgtttcc
     4921 gcacccatat ccgcgcggct cgccaggccg ggcagccgct cgtgatccac acgcgggccg
     4981 catcggcgga cacgctcgcc atcctgaagg aggagggcga agacgggtcc gcgggatccg
     5041 cgggcggggt gtttcattgc ttcacggaga cggccgaggt cgcccgcgcg gcgctcgatc
     5101 ggggcttcta catttccttt tccgggatcc tgaccttcaa gagcgcacag gaactgcgcg
     5161 acgtggccgc gttcgtgccg ctggaccgcc tgctgatcga gaccgacagc ccttatctcg
     5221 cgcccgtgcc ctaccgcggc aagatcaaca acccgtccta tgtgccctac gtggcgcgcc
     5281 aactggcgga actgcgcaaa atgccggtgg acgaagtggc acggctcacg agtgccaatt
     5341 tcgagcatct tttccgcgtc gctttatcat gataaattac ttcaagaaca tcgtttatct
     5401 tattgttata actggatttt ctattgcgta cgcggggtcg tacgaagact ttttcatcgc
     5461 catcaagcag gacaatcccg cagccgtcag gacgctgctg cagcgcggct tcgacgccga
     5521 cacgctcgac ccgaaaggcc tgagcggtct ttacctggcc ctgcgtgacg ggtcgctcaa
     5581 ggccgcccag acgctgatcg actggcccaa gacgaatatc gagatccgta cggcccagga
     5641 cgagagcccg ctgatgatcg ccgcgctcaa gggccatctc gagatcgtca agaagctgat
     5701 cgctcgcgat gccgacatca acaagacggg ctgggcgccg ctgcattacg cagccacggg
     5761 cgggcaccct gcggtcatcg agctgctgtt ggagcaccat gccttcatcg atgccgagtc
     5821 cccgaacggc acgacgtcct tgatgatggc agcccattac ggctcgcaag cggccgtcaa
     5881 gctgctcctg gaagccggcg ccgatccgac catgaggaac cagttgggac ttagcgccat
     5941 cgactttgcc aaccgtgccg ggcgacagga cgctgccgac atggtcgcag cctatgtccg
     6001 caaggctcag ccaaaaggca aatggtgaca atacgctgcg cttgaaagct caggttttgc
     6061 gcgaggtttc ttttcagcgc cgtcccacgc gtttcgccgg gtgctttctg ctgaggcttg
     6121 tcctccatcg tactttatac gatgtatatt atgttaactt atccgtaaag cagcccaggc
     6181 agaatcgtcg atcgatcttg ccaagcttgg ttaccacgag ccctacggtc gcgcgtgctg
     6241 ctgtaaaacc aagacgccgc ggggccgaac cccaggcaag aacctcaagg agcacccatg
     6301 cagtcgatgt ccagcctgtt cattgtttct gcgctcgtcc tggccggttg cgccagccgg
     6361 cctccgggcg aagcaggcac ccatctcgtt taccgcgacg ccagcggcaa acccaccatg
     6421 cagatcgact atccgtccgt ggatttctgc cgcagggtcg aggcgattgc ccgccgcgat
     6481 tcgcgatgcg aggcgaagtc ggcaagcagc gagctgcagg ccagtgccac gcttcgctac
     6541 aaccccggca acatgctcgt cgaaggccac tatccggacg tcgccgcctg ccagcgggcg
     6601 aatgccaaaa tggcccgggg cgtggagctc gccaaaccct gtaccgcgaa gtaacaggag
     6661 ggacccaagc gggccctcac gccggaaagg cggaaaccgc acccgacgtg gaaccgggcg
     6721 aaaccgattt gacatggctc aatgagccgg cacgagagtt cgctgcgacg gacagcttat
     6781 gacctatgat cgccggacaa ctagaaacag cctcacgact agttaccaac ttgttgaatt
     6841 tgcctgtggt tttccgttaa ctccatcaca gtttcgactt gcccttttcg tactctgcgc
     6901 ataacttctc atcacatcag cacgtcagtt gttgttcgca gggagataaa agtacatcat
     6961 gcgttggctg aaacccaatc tcagaagcag catctaccaa ctgctgggca accccgttga
     7021 cccttccgag tccatgctgg aaacgggaac cgaggacatt cgcgagtcga tgctggccgt
     7081 actgggcgaa ggcgggccca agcatttccc gcaaatcacg cgacgtatcc ggtatgccaa
     7141 cgacatccag gcactgtggt acctgcgcgg cgacctgatg gccgcgctgg cggcaatgca
     7201 tggtgaagtg acagcgcgcg aaaaggtcgc ctcggtcacg cagatgttca agggcctgtt
     7261 gcccggcgcc ctgaactcaa ggcccagccc cctggtcggc tgagcgcgcg tgccgctagt
     7321 ggcggtgccc ccaaagtatg aaagcgtcgc ggccttccac cgccaggtga actttcgcgc
     7381 ccacgggtaa cgacaccttg gtgggcacat gcccgaacgg caggttcgtg aaaacaggaa
     7441 ccttcgcctg ttctcgcagc cagtcgatca ccgtctgcag gcggtagccc ttgtcatggg
     7501 gcacgggctt gtagttcgtg aaatgcccca gcacgatcgc cttctgccgg gccagcacgc
     7561 ccgcgtacag taattgggtc agcatccgct cgacacggta aggatgctcg tgcacgtctt
     7621 ccaggaacag gatgccgccc ttgatcctgg gcatccacgc cgttccgacg agcgtggcga
     7681 gcaccgcgag gttacctccc cacagttcgc ccttcagttc gatcggccgg accgcggctt
     7741 cggcgcgcgg caactgccag ccggtgccct ccccctgccc gccgaccagg tcctcgaagc
     7801 aagcctgcat gatgtcgtcg ggcttgccct ccacgccgaa gtcctcgccc aaagccggtc
     7861 cggcccaggt gacggcgccg gtttcggcca gcatggcggt ctggaaggcc gtgaaatcac
     7921 tcaccccgac gaatcgcatg cccttgtcga tggccttggc cacggctttg tagcgaatat
     7981 gcggcagcag gcgggtaagg ccgtagcctc cgcgggtgat gagggcgacg tcggcgccgc
     8041 tggcggctgc gcggtggatc gccgccagcc gggtctcgtc gtcgcccgcg aagcgcatgc
     8101 tggtggcgaa caccgcttca tcgagttcga cctcgtagcc gagcgcctgc agccgcgcca
     8161 cgccgcggcg gatcacgact ttgctgcgta ccgcgctcga cggcgaatag acatagatat
     8221 gttttgtcac gggcggaagt atcccaccgc cgcccgggcg atcggctcgc catgctcctg
     8281 aacgaaatgc cctgcctcct cgatcaccat cggctcgggg cagccgcgga tgctttggcg
     8341 caaggcctgc atcgcgggca ggcccagcac ggggtccttc gcgccgatca ccatcatggt
     8401 ccgcccggtc cactcgctgg acatgaacat gcgcgcgtcg cgtgaaatgg ccgctccatc
     8461 ggcatcggga cgatcgggaa ccatggccgg aaaggcccgt agtgcagccc gatggccccg
     8521 gtcggggaac ggtgcgttgt aggccaagca ctcctcggga ctcatctggg cgttgccccg
     8581 cgcgaacaac cgggcgacgt cgaagtcggg atttttcgcg cacatctcgc gccaggccag
     8641 gaaacccggg ctgagggctg tgtcgccggt gcccagcgcg gtgttcatgg ccagcaagcc
     8701 gaggtaacac cgcggcgccg ccattggcaa ggtcaaccca agcaggcccc cccagtcctg
     8761 caccacgagc acgacgttgc gcaggtcgag ccgttcgacc agttccagca gcacctggcg
     8821 atgccagccg aaactgtgga ccgcttccct cttgggcttg tcgctcctgc cgaaaccgat
     8881 catgtccggc gccaccaccc ggtcccccgc agcgaggaac accgggatca tcttccggta
     8941 caggtagctc caggccgggt tgccatgcag gcacagccag gtgagcggcg catccgccgg
     9001 tccttcgtcc aggtaatgca tccgaagacc cgccagcgaa ggcaggtcgc tgaggtaacg
     9061 gggttgccag ggataacccg gcaattcctc gaagcgttcg tcgggcgtgc gcagcgcatc
     9121 gtcgcgcagc ggatcggcga ccaggcgggc gtccagcctt cgagtgcgga agaattcgcc
     9181 cagcagctcg ccgcaggcgt cggccagcac gcccccctgc acccgcgtct ggtgattcag
     9241 cctgggctgg gcaaaaagat cgaccaccga gccggcggcg ccggtcttcg ggtccgcggc
     9301 accgaagacg acgcgccgca gccgcgcatg aagcatggcc ccgctgcaca tcgcacaggg
     9361 ctccagggtc acgtacaggt cgcagccgtc caggcgatag tttcccgacg cctgggcggc
     9421 ctggcgcagg gcgacgattt cggcatgcgc cgtcgggtcg tttcccgcca ccggcgcatt
     9481 ccgcccgacg ccgatgacct ggccgtcgcg cacgaccacg gcacccaccg gcacctcacc
     9541 ggccgccccg gccagccggg cctgctgcag ggccaggccc atgaactgtg cgtcgctcac
     9601 ttgtcctcgg gcatgggccg cgcctgctgc atggcgcctt ggacggcctg ctggaactgc
     9661 cgcacttgct gctgcggcgt tccagcgggt gcggccatgc tgccgctggc tggcgtggag
     9721 gcaggcgccg cgaccggcgc ggtcgccccc aattgcttgc gagccagcaa gccgacgatg
     9781 gccacgacca caagaaggct caagattccg aaaacggcgc gcatcttgcc atcctcctac
     9841 ttttcttcct gccgtccaag gacggcctgg ccggccgccg gcgaatgcac gggctcgccg
     9901 atgcccagga cgtccatcca ttgcgccagt tcgcgctcct gctcgttgcc gcgggcataa
     9961 ccgtcccgca gggccgcgtg cgattcgggg cggtgctggt tcagcttcag cttgcattcc
    10021 agtgccgtca cctgcaactc gaaagccatg atgccggcca gcatcttgcg cgcgtagtcc
    10081 tcgcccaggc tgcgccattg ctgcgcatac ggcggttcgt ggtcgccgat cagtttcttg
    10141 agcagcctgt ccttgaccag tggctcgtcg accagcgtgg cggtcacggt gcaatgcacg
    10201 gccaggtaat tccagctcgg cacccgcgcc aggtccgggt agatcctcgg tgactggtag
    10261 gcatgcggtc ccaggaaggt aaccaccgcc gtcgggcgct gctgcagata gcgccaatgc
    10321 ggattggatc tcgcgcagtg gcccagcaga acaaagccgg gccgctccga agccccggaa
    10381 gcctcccggt cttccaggtg caggggcagg tgcgtgacga acggcaggcc ggcatcgtcg
    10441 ttggagatca ggctggcaaa ggggtgctcg cgcatcagcc gtgcggcgat ggcacggtcc
    10501 gtggacctga attgaggagg ctggtacatg gggtggattg tgccccgcag tcttgattag
    10561 actgtctaaa tgatgagttg gactgtataa caaatcacct tgacagtcta cttaatctgt
    10621 ctaaactaca tggacagatc aaacgcagag gccctcttgt tgaacagcaa agagctactg
    10681 gaaaagaccg gcatctcccg ggcaaccctg aacaactaca tcagttgggg aatcgtgccg
    10741 aaaccggaag tgctgccgcc ggagccgcag gacggcgccg cgccgcgcat cggctacttc
    10801 ccggacgagg tcgtgcagcg tatcgaagac atacagcggc tcaagcgcga gggctggagc
    10861 atcacccgca tcgcggaaca tttcaatgcg gcagcgggcg atccgtcgaa cgaggctgcc
    10921 ggcactgcca tggcaagccc agtgccgaag cgcggcgcga tgcccaacgt gtcgatcgat
    10981 gaaatcacca accccgcgta catagtcaat caccgcctcg aagtaacctg gttcaacgcg
    11041 gccgcgggcg cgggcgtgct tcgagacctg ccgcagtcgc cagaggacgc cgcctcgcgc
    11101 aacgtattca ggtacctcct gcacagcccg tcaacggacg caaacagccg ggagcaggtc
    11161 ctgcgcttcc atctcgggct cgccaagcag catggggcga gtttctccct cctctgccgc
    11221 gaagtgcccc gggaggaagt gggctggttg aagcgcctcc acgatgaggc gaagcagccg
    11281 gagccgggcg tggtctccca tgccctcctc ccaggcggtg gcgccgggct cgcgtcatcg
    11341 gtatgcgtct atgcggttca gttccgcgaa ggcattctga tcctgtatgt tccgggcggc
    11401 cgtgcttccc aggtcccgtc gcaacccctt gtgcagcgtg agcttgtgac ccccgaagcc
    11461 ggcggcaagg tacagcccat gctaagccgc gcggcggtgc tggcagccga cctgcagcac
    11521 ccccaccgga tctggtccga gctgcccccg gaggaatatt tcgaactgat caaccagatc
    11581 tggttgacgg ttgaacccat tttcaagcgt cacaacggca cccatggcaa gcaccaccgg
    11641 gatggcgtgg tttgctactt cttcccgcag cccgatggca gccacgtgtg gaatgccctc
    11701 gtcgcggcgc tcgaaatgcg cgacgcgatg cgccgcgtga gcaaggcatg gcaactgcgc
    11761 aagggctgga ccaccgagct ttacatgaac accggcatcg acgaggggcc gcagtggctg
    11821 ggcacgttga agtccccgcc gaaggcggag ttcaccatgc tcggcgatac cctgaaccat
    11881 gcggtccgga tctcggattt cgccagcttc ggctccatct gggtgaccaa gaacctgatc
    11941 ggcaaactca ccacggacga aaagcagcgc ctgaaatacg gtgtgcggcg caggagcaac
    12001 gacggccgcg acatctttgt ggcgtccgtt ttctcgaacg tggacagcct gaccgacgcg
    12061 gcagcgggtc ccgccgaaaa gctcaagggc attgcccggc tgccgatcac ggaggtcgtc
    12121 gaaatcgccg ttccggacag gcgggtggat ctcgcggccg agcacacgtt ggcctgatgg
    12181 aaaatagcat gggctggttc aagacccata tcgtcagcac catttcggcg ctattttcgt
    12241 cagggctttc gtcggcaccg gcggaattgg agatggaggt ggggatcgag gaaattcgcg
    12301 ccgccatgct cgaccttatc actgacgtcg acgacaagaa gttcatgccg gtggtgcgcc
    12361 gcatccgcta cgccacggat gtcctgagct tgtggtatct gcggggcgac ttgatggcag
    12421 cccttgccgc caggtatggc gaggagcagg cacgggagag cctgaagccg atcaccggga
    12481 tgttcaccaa catgctgccg ccgggattga ggtcgcgtgt cagtccgctt gcctccgcgt
    12541 cgagagactg gcaggccagc gaccgcaaca gcgacctgcg gaaatgaccc caaatcagcg
    12601 ggtcaacgtg attgcccgcc cccgcggggc tccacggact attcccattc gatcgtggcc
    12661 gggggcttgc tgctgacgtc gtaagtcacc cggttgatgc cccgcacctc gttgatgatc
    12721 cggctggaga ccttcttgag caacgggtac ggcagttcgg cccagtccgc cgtcatgaag
    12781 tcgctggtct gcacggcgcg cagcgccacc acatagtcgt aggtgcggcc atcgcccatc
    12841 acgcccacgc tcttgaccgg caggaacacc gcgaacgcct ggctggtaag gtcataccag
    12901 ctcttgccgg tggccttgtc ctcgcagtcg cgcagctcct cgatgaagat ggcgtcggcg
    12961 cggcgcagca ggtcggcgta ttcctttttg acctcgccca ggatgcgcac gcccaggccg
    13021 ggcccgggga atggatggcg gtagaccatc tcgggcggca aaccgagggc cacgcccagc
    13081 tcgcgcacct cgtccttgaa caggtcgcgt agcggctcca gcagcttcag ccccagctgc
    13141 tccggcaggc cgcccacgtt gtggtggctc ttgatggaca cggccttctt gttcttggcg
    13201 cccccggact cgatcacgtc cggataaatg gtcccctggg ccagccaggc cacgtgccgc
    13261 gcgtgcgtct gcttgatctt cgcggcctcg gccttgaaca cttcgacgaa ctcgcggccg
    13321 ataatcttgc gcttggcttc cggatcgccg acgccggcca gcttgcccag gaacagctcg
    13381 ctggcgtcga ccctgatcac cttggcgtgc agcttgccgg cgaacatctc catcaccatg
    13441 tcgccctcgt tcaggcgcag caggccatga tccacgaaca cgcaggtcag ctgctcgccg
    13501 atcgcgcgat ggatcaacgc ggctgcgacc gaggagtcga cgccgcccga gagtccaagg
    13561 atcacctcct cgtcgcccac ctggcgccga atcagatcca cggcctcggc cacgtggtcg
    13621 tgcatgaccc agtcggaccg cgcgccacag atatcgagaa cgaagcgctc cagcatggcg
    13681 cggccctgcc gggtgtgcgt gacctccggg tggaactgca ggccgtagaa accacgagcc
    13741 tcatcggcca tgccggcgat agggcagctt tcggtgctgg ccatcagctt gaagcccggc
    13801 ggcagctcgg tcaccttgtc gccatggctc atccagacgt cgagcattcc atggccctcg
    13861 ggcgtgttga agtcggcgat gtccttcagc agcctcgtgt ggccgcgcgc gcgcacgctg
    13921 gcgaaaccga actcccgctt gtggccaccc tccaccttgc cccccagctg gtgcgccatc
    13981 gtttgcatgc cgtagcagat gcccagcacg ggaacgccca gttcgaacac cgcttgcggc
    14041 gccttgtcgg tggtgtcttc gtacacgctg gcgtggctgc ccgagaggat cacgcccttg
    14101 agccggccat cggccgcgta ctcgcgtacc cactcgcccg tcacgtcgca gggatagacc
    14161 tcgcagaaca catgtgcctc gcgcacgcgc cgcgcgatca gctgcgtgac ctgggagccg
    14221 aagtcgagga tgaggatctt gtcgtgttgc atgggtcagg gcaggtcggt gatgacagga
    14281 tgatcgagct ggaggcaggc gcgttgcaga ttgcggtcca tggtgaccag cggcgccttc
    14341 aagtcaaccg ccagtttgag ataagccgcg tcataagcgg tgagttggag tgattgcgac
    14401 cacgaccaga actcgagcgg cgacaggcgc agcggcgcga gggcaactgg cagccggctg
    14461 agcagccgca atacatgctg gtgaacggtg ggcgtgaagc ggccacgggc caaagcgcgg
    14521 ccggccgcgt gcaacgcctc cagcgggaaa atggcggcac tgcttgcgct ttcctcttca
    14581 gtcatgatcc gcaccagtga ctgtgaaacc ttgtcgccgt gctcgtcggg aaaagcccag
    14641 ctggcgaacg cggaggcgtc aacgaccagc attgccgccc tttgcctgcg cggcgtcgat
    14701 ccatggatcg ggccgttcgt gcaattcccg cttgtgctcc tcccacaatt cgctgaaacc
    14761 aggctgaccc gaaccgactt tgcttcgcag cgcttccagt tcggccatga tcgcctggcg
    14821 gcgggcctcg acgctgggct gaaccgactc gggctccttg atcaggcgaa tcacccgttt
    14881 gccatggcgc gtcaatacga cttcctcgcc ctgctcgacg gcgcgaatca gttcggacag
    14941 ttggcttttg gcttgatgga tgggtacggt ctgcataatt ctggtctgtt cttctgaagt
    15001 tgggccatat tttaacccaa tcatgaaaaa agggccagaa cgttctgacc cttttttgta
    15061 gatcgtaaat cggggttctc aatcagcgcg gtaattcggc gcttccttgg taatctgcac
    15121 gtcgtgcaca tggctctcgc ggatgcctgc aacggtgatc tcgacgaatt ccgccttgct
    15181 cttcatctct tcgatggtcg cgcagccgca atagcccatg ctggcgcgca ccccgccgga
    15241 catctggtaa acgatcgcca ccagggagcc cttgtacggc acccggccct cgatgccttc
    15301 gggcacgagc ttgtcggcgt tcgggtttcc cgtggtcgct tcctggaaat accggtccgc
    15361 gctgccctgc tccatcgcgc cgatcgaccc catgccacgg tagctcttgt agcttctgcc
    15421 ctggaacagg acgatctcgc ccggggcttc ctcggtgccg gcgaacatgc tgcccatcat
    15481 cacggtgctg gcgccggccg cgatggcttt ggagatgtcg cccgagtagc ggacaccgcc
    15541 atcggcgatc agcgggacgc cgctgccttg cagcgcggtg gccacattgt cgatggccgt
    15601 gacctgcggc acaccgacgc ccgcgacgat gcgcgtggtg cagatcgagc ccgggcctat
    15661 ccccaccttc accgcatcgg cgccggcttc caccaaggcc cgcgcggccg ccccagtggc
    15721 aatgttgccg ccgatcacgt ccacttgcgg gtagttcttc ttcacccaac gcacgcgctc
    15781 gatcacgccc ttgctgtgcc cgtgcgccgt gtccaccacg atcgcgtcga cgccggcctt
    15841 gaccagcgcc tcgacgcgct cctcggtgcc cacgcccacg cccacagccg cgccgacacg
    15901 cagccggccc gaggcgtcac gcgccgcgtt cggaaaactg gtctgcttcg tgatgtcctt
    15961 gacggtgatg aggcccttga gctcgaaggc gtcgttgatc accagcagcc gttccagctt
    16021 gtacttgttg agcagcgcct tggcttccgc cgcgctggtg ccttccttga cggtgatgag
    16081 cttctcgcgc ggggtcatga tgtcgcgcac cggcacgtcg tagcgttcct cgaagcgcag
    16141 gtcccggccg gtgacgatgc ccacgacctt gccgccgtcg atcacaggaa agcccgaaat
    16201 gcccagttgc acctgcatgt cgatcacttg ccgcacggtg tggccgggcg tgatgacgac
    16261 cgggtcgcgc agaacaccgg actcgtagcg cttgactcgg gaaacctcgg cggcttgctg
    16321 cttcgcggtc aggttcttgt gcacgatgcc catgccgcct tcctgggcga tggcgatcgc
    16381 aagccgtgcc tcggtcaccg tatccatcgc ggcggacacc agcggcaggt tcagggtgat
    16441 gtttcgggag aggcgtgtcg ccagggaagt gtccttgggc aggacctggg agaacgccgg
    16501 caccagcaac acatcgtcga aggtgagcgc ttttcctaag aggcgcatgt ctaagctcca
    16561 aaagatggat tgtacccgtg gcccctcggt acttaccctc gcaaggagat gccgggacgg
    16621 cgtcaatcgc gcgcaacact cccgcgacga agatgtcgcg tggtcgcgcc ccggcgctac
    16681 actggcggaa tgttattgct ccgcattttc gtgctcggcc tggcatgcgc gctgccggcc
    16741 atctcattgg cccaatggca gtggatcgac aaggacggcc gcagggtctt cagcgaccag
    16801 tcgccgccgg ccgacatccc agccaagaac atcatcaggc agcccggcgt caagagccct
    16861 tcgcccgaac ccgtggcggt cgccgacccg cagccggcca agccggccgc ccctaagctc
    16921 agcggcaagg accaggcgct ggaacaaaag aaaaagcagg ccgaggccgc cgaagccgag
    16981 aagaaaaagt ccgatgaaga acggatcgcc aaggcccgtg ccgacaattg cgaacggagc
    17041 aaacgcgcca aggccggctt cgattcgggc gtcaggattg cccgcatcaa tgccaagggc
    17101 gaacgcgaat acctcgacga cgccgcccgc gccgcggaga ccaagcgcct cgaaggcatc
    17161 atcgccggcg actgcaagcc ggcgggcgct cagtagggct tctcgagccg ctaagtcctg
    17221 cgcgcgaaca gtcctgcgga gcgcacgccc tgctggcgga agcgctcccg ccgcgcgagc
    17281 ttcggatcca ccttgagcgg ccggtagacc tcgacacggt cgccgtcgcg cagcgcctgt
    17341 tcgaggccgg ccttgcgtcc ccagacgccg gcggcggcgc tgcgaaggtc cagatccgga
    17401 aacgcctcat tcagtccgca ggccagcagg gcccccagca cggtggaacc gggcgccagc
    17461 accaacggcc actcgtggac ctcgcgcggc gccggcgagt acaccaccac agcccggatc
    17521 tcaggtcgag ccatacacct gttccgcgcg cttgacgaag gcctcgacca tgctgctcgc
    17581 gatcttgccg aacacgggac ccaccagcga cgctagcgcg gcattgtcga agccgtaatg
    17641 cagctccagt tccactttgc aggcgcgctg ctcgggggtg ccaagtggag tgaacttcca
    17701 ctgtccatcg agccgcgaaa aaggaccttc gatcagcttc atggacacct cgcgccccgg
    17761 cacgtgttcg ttgtgcgtcc ggaaggtctg atgaatgccg ccgaacgcga tcccgatctc
    17821 ggctttcatg ccctcaccct cgggaacgac cgacgcgtgg tcgcaccatg gcaggaactc
    17881 cggatatcgg tcgacatcgg tgaccagcgc gaacatttcc tcggcgctgt accagatgag
    17941 gacggatttg tgaacggttt tcatagagaa tgcggagccc gctgggcatt gtagagacgc
    18001 gccggcaccc catttgtaag ccatggccaa gaaacccgag acatccagcc gaattgccga
    18061 caacaagaaa gcggcctaca actatttctt cgaggagaaa ttcgaggccg gcatggtgct
    18121 gcaaggctgg gaagtcaagg ccctgcgcga gggcaaggtg cagctcaccg acggctacgt
    18181 ggtggtcaag aacggggagc tgttcgtgat cggctgccag atcaatccgc tgaaaaccgc
    18241 ctccacccat gtgaaccccg atgccgtccg gaccaagaaa ctgctgatgc acaaggatga
    18301 aatccgccgc ctggtcggca aagtcgagca gaaaggctac acgctggttc cgctcaacct
    18361 gcactggaaa gagggcaagg tcaaatgcga gatcgccctg gccaagggca aggccgagca
    18421 cgacaagcgc gacaccatca aggaccgcga aggcaagcgc gaagtggagc gcgcgatgaa
    18481 aagccgcaat cgctgaaggc cgggttttct cgcttcgatg gaataaaccg gtggccgccg
    18541 gtgttcatgc gtctggaagg ctaatcaccc accggaggtt ccatccgcac accgcttgcc
    18601 gtgctctgcc gcacgatccg attggccggt ggcatcattg atggcgcagt gcggtccgcg
    18661 ggcgatcggg gccctgctct caatgcgcca tgaaccggat actgcttgtc gaacagctcc
    18721 ccggcctgcg ccgctatgcg cgggcgctga ccggcgatgc ctgggccgcc gacgacctgg
    18781 tgcaggacac gctggagcga gcctgcatca aatggcagct ctgggtggcg ggcacggacc
    18841 tgcgcgcctg gctgttcacg ctgatgcaca acctttacct caaccagcgg cgcgcggcca
    18901 gggtggcgct gaacagcgtg agcatcgacg acgtggaagc cgagctgcgc gctccggctg
    18961 ccggaatcga cggtccgctg gacctcgacc gctgcctgca gcggctgcct gcggaccagc
    19021 gggccgtgct gttgctggtt gcactggaag acatgagcta tgaagacgcg gccaaggtgc
    19081 tggccatccc gctgggaact gtgatgtcgc gcctgtcgcg tgcgcgcagc cgattgcgcg
    19141 agctgatgga aggcggcaag cccgcggcct ggacggcggc cccggcgccc ggcttgcgcc
    19201 gcatgaagtg atggagtgac gatggacccg cgccaagacg accctgcccc gattcccgag
    19261 gacgacctgc acgcgctggt cgacgcccgt cttgcgcccg aagatgccgc ggccctgcgc
    19321 cggcgcctgg ccgcggaccc gcaagccaaa gccaccgcag cggcgtggca ggcccagcgc
    19381 gacgagctgc ggggtcgcta tgcgcaaatg gaggaccttc cgctgcccgc ctccctggtg
    19441 gcgacggccc aacgcgccga gcgggcgctc gcgcagacgt cgcagtggtg gcgatggggc
    19501 ggcatggccg cggcgctgct gctggcgttc ggcatgggtt ggctcgccag cagtcaatgg
    19561 cgcaccgacc acggcgatac ggccctggca cgctcggagc cggctcggca tttcgcccgg
    19621 caggcggcga tggcctacgc cgtattccag cccgaggtgc gtcatccggt cgaagtcacg
    19681 gccgcgcagc aggaccacct ggtgcaatgg ctgtccaagc ggctcggccg gcagctcaag
    19741 gtgcctgtgt tgacggcgca aggctatgaa ctcgtgggcg ggcgactgct gcccggtgac
    19801 gatggcggcg cccgcgcgca gttcatgtac cagaacagcg cgggccagcg gatcaccctc
    19861 tatctgggtg cggtgaaatc ggatgtgggc ggggcggcgc gcgagaccgc gttccgcttc
    19921 ctgcaggagg gcccggtgcc ggggttttac tgggtcgacc agggctttgg gtacgccatc
    19981 agcggcacgc tgccgcgtcc ggcgctgcag gagttggcca aagtcgtgta cggccagctg
    20041 tagaaacgtt tcaggcccgc tgtttccagc ccaccagttc attgaccacc aaggcgccaa
    20101 gcaccagcgc gcccccggcg atcaccgtgt ggctcggcac ctcaccggcc ccgacccatg
    20161 cgagcaggat gccgaaaatc acctcgagcg aggccagcag cgcgatctcg ggcgccttga
    20221 ggacgcgggc gcacaccacc gaaagcgagc agggaatggc cagctggaac aggcccagca
    20281 ccgccagcag cccgatgtcg tggccggatg cctgcaaggg catggccggc ggaagcatcg
    20341 tcaccgttga aatgatcgca ccgacgagca ccgcgggcac caggtccacg ttcaggccgc
    20401 gggcattcga gcgctgcacc acggtccagt tcactgcccc cgccagcggc acgcacagcg
    20461 cgaccagcgt accggcgagt tgcccgcccg aaacctgcgc ggcgtacatg taggcgatgc
    20521 cgctccccgc caccaggatg gccagccacg tccgcacggg tacgcggtgg ccgatgaaga
    20581 cgcgcgccat cagggccgtg aggaagggac ccacggacat cgtcaccagc acgttggcca
    20641 cgctcgtcag cgtgagcgcc accatgaacg ccgtgaacat gacgctccag cacatgccgg
    20701 aaatccacaa cgcgggcccg ccatcgcgaa tgcgcgcaaa aacctgccgg ccctggaaca
    20761 ggggcaggat caccatgagc gatgccacgg tgaagaaact gcgccagaag gtgatctcga
    20821 aactgcgcgc gtgctccagt tgccg
//
LOCUS       DASWIY010000251         5609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2753567, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000251 DASWIY010000000
VERSION     DASWIY010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2753567"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VGA59_09240"
     CDS             <1..252
                     /locus_tag="VGA59_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9719903.1"
                     /translation="CAVYAREGITGERDPSTIGFATIRGGDIVGDHTVLFAGTGERIE
                     ITHKSASRATYAQGSLRAVRFLAGKRSGMFDMFDVLGLR"
     gene            341..1093
                     /locus_tag="VGA59_09245"
     CDS             341..1093
                     /locus_tag="VGA59_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9719904.1"
                     /translation="MSFVELFTQGDAVTRVVAVLLLAMSVASWVVILWKAWLLRRARR
                     DISRSTAAFWQSASVEQARQVVGAFDREAFVLPLIAATEMDGAGSLASAGDRSQQLTR
                     LLRDALHLVLDKLQFGQVLLASVGSTAPFVGLLGTVWGIYHALTGIAAAGQITIDKVA
                     GPVGEALIMTAAGLAVAIPAVLGYNVFGRLIGRLEADLEGFARDLRELLTHAAAPAGS
                     KLAAASAAGAPQGERAARGGSAAASAASVEAL"
     gene            1090..1518
                     /locus_tag="VGA59_09250"
     CDS             1090..1518
                     /locus_tag="VGA59_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9719905.1"
                     /translation="MSFGRLERSQGPQPMSDINMTPLVDVMLVLVVIFIITAPLLGSS
                     IRLDLPRTEAAKPNEAPKFVTVVLDKAGGVFLEDKPVVGLAQLAEQLAQAARRNPETE
                     VQLRADQAVPYGRVVEVMGAAQKAGLSRIGFVAEPPQPAR"
     gene            1568..4201
                     /gene="leuS"
                     /locus_tag="VGA59_09255"
     CDS             1568..4201
                     /gene="leuS"
                     /locus_tag="VGA59_09255"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX9719906.1"
                     /translation="MQEKYNPSEVERSAQAQWNARDAYRVTEAAGKKKFYACSMLPYP
                     SGKLHMGHVRNYTINDMLTRYLRMNGHNVLMPMGWDAFGLPAENAAMKNGVPPAKWTY
                     DNIAYMKRQMQAMGLAIDWSREVATCDPGYYKWNQWLFLKMLEKGIAYRKTQVVNWDP
                     VDQTVLANEQVIDGKGWRTGAPVEKREIPGYYLKITDYAEELLDHVQHKLPGWPERVK
                     LMQENWIGKSEGVRFAFTHGIRDFNGDLIGDGRMYVFTTRADTIMGVTFCAVAPEHPL
                     AVHAARTNPRLTAFIEQCKKGGTTEAELALRDKEGLPTGLVVFHPITGEPIDVWVGNY
                     VLMGYGDGAVMGVPGHDERDFAFALKYNLPIMQVVHVDGEHYDYKHWHDWYADKEIGV
                     TINSDIYSGFSYQDAVNAIAHALQVKGLGGKMITWRLRDWGISRQRYWGTPIPIIHCE
                     EHGAVPVPEKDLPVVLPPDCVPDGSGNPLNKRADFLNVPCPVCGTPARRETDTMDTFV
                     DSAWYYMRYCDSKNDEAMVAGGADYWMPMDQYIGGIEHAILHLLYARFWTKVMRDFGL
                     VKIDEPFTKLLTQGMVLNEAFVRVGEKGKQYYWTHELDIVRDEHAKVISATAKADGLP
                     VSYEGWTTMSKSKNNGVDPQDLIEKYGADTARLYTMFTAPPEATLEWNDAALEGSFRF
                     LRRVWNFGCKLASAPADGHAPAKPAAAGKQARALRFEIHSVLKQVDYDYQRMQYNTVV
                     SGAMKLLNALEDFKGTTEPGAEAAVREGFGILLRVLYPVTPHIAHELWHALGYGQRQG
                     ELLDAPWPKVDGDALKQDEIELVLQVNGKHRGSILVPATASRQQIEKLAVASEAFAAH
                     AAGAVPKKVIVVPGRLVNVVI"
     gene            4374..4886
                     /gene="lptE"
                     /locus_tag="VGA59_09260"
     CDS             4374..4886
                     /gene="lptE"
                     /locus_tag="VGA59_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX9719907.1"
                     /translation="MKNPAWRRRNLLALGVALALSACGFQLRQAPTFAFSTIYIGAAP
                     TSTLGNELKRSIASSDAVTVVGDAARLESAQVVLDVLADQREKVVVGLNASGQVREFQ
                     LRTRLRFKLRTPQGKELIPETELLQQRDISFNESAVLAKESEENLLYRDMQTDLVQQL
                     MRRLAAVKTL"
     gene            4889..5368
                     /locus_tag="VGA59_09265"
     CDS             4889..5368
                     /locus_tag="VGA59_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9719908.1"
                     /translation="MELDRADLTLLEMLQRDGRTTIQALSEAIHLSARATLNRVRKLE
                     AAGVIEGYRALINRAALGEQISVFAEIALKDQRQSAVQRFEKRMAATPEVVACYVISG
                     RYDYLVRIACPHLAQYHRLISGWLDEAQLGVEKIVTNIELQTIKEFAGFPLPRPGRL"
BASE COUNT         1024 a         1837 c         1894 g          854 t
ORIGIN      
        1 tgcgcggtgt acgcgcgcga gggcatcacc ggcgagcgcg acccatccac catcggcttc
       61 gccaccatcc gcggcggcga catcgtgggc gaccatacgg tgctgttcgc cggcaccgga
      121 gagcgcatcg agatcaccca caaatccgcc agccgggcca cctacgccca gggcagcctg
      181 cgcgccgtac ggttcctggc cggcaagcgc agcggcatgt tcgacatgtt cgatgtgctc
      241 ggcttgagat gaaagcccac ccccgaagcg ccttcggttc cgcggtggcc cccgcactaa
      301 ccgcgcgaat tcgtactcgc tcggcgagac cggcgcgggc atgagttttg tcgaattgtt
      361 cacgcagggc gacgcggtca cgcgggtcgt ggccgtcctg ctgctggcca tgtcggtggc
      421 cagctgggtg gtcatcctgt ggaaggcgtg gctgctgcgc cgcgccaggc gcgacatcag
      481 ccgcagcaca gccgccttct ggcagtcggc atcggtggag caggcccggc aggtggtcgg
      541 cgccttcgac cgcgaagcct tcgtcctgcc gctgatcgcg gcgaccgaaa tggatggggc
      601 cggctcgctg gcgtcggcgg gcgaccgctc gcagcagctc acgcggctgc tgcgcgacgc
      661 cttgcacctg gtgctggaca agctgcagtt cggccaggtc ctgctcgcca gcgtcggttc
      721 cacggcgccc tttgtcgggc tgctgggcac ggtgtggggc atctaccatg cgctcacagg
      781 catcgcggcg gcgggccaga tcacgatcga caaggtggcc ggaccggtcg gcgaggcgct
      841 gatcatgacg gcggccggcc tggcggtggc tatccccgcc gtgctcggct acaacgtgtt
      901 cggccgcctc atcggccgcc tcgaggccga cctggaaggt tttgcccgcg acctgcgtga
      961 gctgctgacc catgcggcgg cgcctgccgg cagcaagctg gcggccgcaa gcgccgccgg
     1021 ggcgccccaa ggcgagaggg ccgcccgcgg gggcagcgcg gcggcttcgg ccgcgagcgt
     1081 ggaggccttg tgagtttcgg ccggctggag cgcagccagg gcccgcagcc catgagcgat
     1141 atcaatatga cgccgctggt ggacgtcatg ctggtgctgg tggtgatatt catcatcacc
     1201 gcgcccttgc tgggcagctc gatccggctc gacctgcccc gaaccgaggc cgccaagccc
     1261 aacgaggcgc cgaaattcgt taccgtcgtg ctcgacaagg ccggcggggt attcctggag
     1321 gacaagccag tggtggggct ggcgcaactc gccgagcagc tggcccaggc ggcgcggcgg
     1381 aaccccgaaa ccgaagtgca gctgcgcgcc gaccaggcgg tgccgtacgg ccgtgtggtg
     1441 gaagtgatgg gcgcggccca gaaagcgggc ctgagccgca tcggtttcgt cgcggagccc
     1501 ccgcagccgg cgcggtaatg cgcccgtaga atccgtgcag gccgggcgaa acagccggca
     1561 cacctatatg caagagaaat acaacccttc cgaggtggag cgttcggcgc aagcgcagtg
     1621 gaatgcgcgc gacgcctacc gcgtgaccga ggctgccggc aagaagaagt tctacgcctg
     1681 ctcgatgctg ccctacccca gcggcaagct gcacatgggc catgtgcgca actacaccat
     1741 caacgacatg ctcacgcgct acctgcgcat gaacggccac aacgtgctca tgccgatggg
     1801 atgggacgcg ttcggactgc ccgccgaaaa cgcggcgatg aagaacggcg tgccgccggc
     1861 gaaatggacc tacgacaaca tcgcctacat gaagcggcaa atgcaggcga tggggctggc
     1921 gatcgactgg tcgcgcgaag tcgccacctg cgatcccggc tattacaagt ggaaccagtg
     1981 gctgttcctg aagatgctgg aaaagggcat cgcctaccgc aagacccagg tggtgaactg
     2041 ggacccggtg gaccagaccg tgctggccaa cgagcaggtg atcgacggca agggctggcg
     2101 cacgggcgcg ccggtcgaga agcgcgagat cccgggctac tacctcaaga tcacggacta
     2161 cgccgaggag ctgctggacc atgtgcagca caagctgccg ggctggcccg aacgcgtcaa
     2221 gctgatgcag gagaactgga tcggcaagag cgaaggcgtg cgcttcgcct tcacccacgg
     2281 catccgcgat ttcaatggcg acctgatcgg cgacggcagg atgtacgtgt tcacgacccg
     2341 cgcggacacc atcatgggcg tgacgttctg cgccgtggcg cccgagcatc ccctggccgt
     2401 tcacgcggcc cgcaccaacc cgcgcctgac cgccttcatc gagcaatgca agaagggcgg
     2461 caccaccgag gcggagctgg cccttcggga caaggaaggc ctgccgacgg ggctggtggt
     2521 gttccatccg atcaccggcg agccgatcga cgtgtgggtg ggcaattacg tgctcatggg
     2581 ttacggcgac ggcgccgtga tgggcgtgcc cggccacgac gagcgcgact tcgccttcgc
     2641 cctgaaatac aacctgccga tcatgcaggt ggtccacgtg gatggcgagc actacgatta
     2701 caagcactgg cacgactggt acgccgacaa agagatcggc gtgaccatca attcggatat
     2761 ctacagcggc ttttcctacc aggacgcggt caacgcgatc gcgcatgcgc tgcaggtgaa
     2821 gggcctgggt gggaaaatga tcacctggcg gctgcgcgac tggggcatca gccgccagcg
     2881 ttactggggc acgccgattc ccatcatcca ctgcgaggag catggcgcgg tgccggtgcc
     2941 cgagaaggac ctgcccgtgg tgctgccgcc cgattgcgtg ccggacggtt ccggaaatcc
     3001 gctgaacaag cgcgcggatt tcctgaacgt tccgtgtcct gtatgcggca cgccggcgcg
     3061 gcgcgagacc gacaccatgg acaccttcgt cgactcggcg tggtactaca tgcgttactg
     3121 cgactcgaaa aacgacgagg cgatggtggc cggcggcgcc gactactgga tgccgatgga
     3181 ccagtacatc ggcggtatcg agcacgccat cctgcacctg ctctatgccc gtttctggac
     3241 caaggtgatg cgcgacttcg gcctggtgaa gatcgacgag cccttcacga aactgctgac
     3301 gcagggcatg gtgctcaacg aagcattcgt gcgcgtgggc gagaagggca agcagtacta
     3361 ctggacccac gagctggata tcgtccgcga cgagcacgcg aaggtgattt cggccacggc
     3421 caaggccgac ggcctgccgg tgagctatga aggctggacc accatgtcca agtccaagaa
     3481 caacggcgtc gatccgcagg acctgatcga gaaatacggc gccgacacgg cgcgcctgta
     3541 caccatgttc accgcgccgc ccgaagccac gctggaatgg aacgacgccg ccctggaagg
     3601 gtcgttccgc ttcctgcgcc gggtctggaa cttcggctgc aaactggcga gcgcgccggc
     3661 ggatggccac gcgccggcga aaccggcagc cgccggcaag caggcaaggg ccctgcgctt
     3721 cgaaatccac tccgtcctca agcaggtgga ctacgactac cagcgcatgc agtacaacac
     3781 cgtggtgtcc ggcgcgatga agctgctcaa cgcgctggag gacttcaaag gcacgaccga
     3841 gccgggcgcc gaggcggccg tgcgcgaggg gttcggcatc ctgttgcggg tgctttatcc
     3901 ggtgacgccg catatcgcgc atgaactctg gcacgccctg ggctacgggc aacggcaggg
     3961 ggaactgctc gacgcacctt ggcccaaggt cgacggggac gcgctcaagc aggacgagat
     4021 cgagctggtg ctgcaggtca acggcaagca ccgcggctcg atcctggtgc cggccacggc
     4081 cagccgccag cagatcgaga aactcgccgt cgccagcgag gcctttgccg cgcatgcggc
     4141 cggggccgtg ccgaagaaag tcatcgtggt gccgggacga ctggtgaacg tggtgatctg
     4201 agaatgaaat cccacccccg aagcgccttc ggcgatgccc cctcgggctt cgccctctcc
     4261 ccctcgaggg ggcgccacca gcggcccggc aaagccggtt ccgcggtggc cctcgcactg
     4321 accgcacgag ttcagacgct gcgggtggcg cgaagcgcac tggacaaact gacatgaaga
     4381 accccgcttg gcgacgcaga aacctgctgg cactgggcgt cgcgctcgct ctatccgcct
     4441 gcggcttcca gctgcggcag gcgccgacgt tcgcgttcag caccatctac atcggcgcgg
     4501 cgcccacttc cacgcttggc aacgagctca agcgaagcat tgcctccagt gatgcggtga
     4561 cggtggtcgg cgacgcggcg cgcctggaat ccgcgcaggt cgtgctggac gtgctggcgg
     4621 accagcgcga aaaggtcgtg gtgggcctga acgcttcggg gcaggtgcgt gaattccagc
     4681 tgcgcacgcg cctcaggttc aagctgcgca cgccgcaggg caaggagctg attcccgaaa
     4741 ccgagctgct gcagcagcgc gacatcagct tcaacgagtc ggcggtgctg gccaaggagt
     4801 ccgaggaaaa cctgctgtat cgcgacatgc agaccgacct cgtgcagcag ttgatgcgca
     4861 ggctggcggc cgtcaagacg ctctagcgat ggagctcgat cgcgcggacc tgacgctgct
     4921 cgagatgctc cagcgcgacg ggcgcaccac gatccaggcg ctttcggaag ccattcacct
     4981 gtcggcacgg gccacgctca atcgcgtgcg caagctggag gccgcaggcg tgatcgaggg
     5041 ctaccgcgca ctgatcaacc gcgccgcgct gggcgagcag atctcggtat tcgccgaaat
     5101 cgcgctcaag gaccagcggc agtcggccgt gcagcgcttc gagaagcgca tggcggccac
     5161 gcccgaagtc gtggcctgct acgtgatcag cggtcgttac gactacctcg tgcgcatcgc
     5221 ctgcccccac ctggcccagt accacaggct gatcagcggc tggctggacg aggcgcagct
     5281 gggcgtggag aaaatcgtca ccaacatcga gctgcagacg atcaaggaat tcgccggctt
     5341 cccgctgccc cgcccgggcc ggctctagga gcctgggcgg cagggaaacg ggcctgcgcc
     5401 gacggcatcc cggcctgcac ggcaatcaag gcgcgtccta caccacttcg ctttcggaag
     5461 tgcgacacct tcattcatgc cgttgccgcc gtgcgcgccg gctcgattca cgctcacggc
     5521 agcaaggtcg ggcctagact ttccttatcg aacggggaaa ccagcatgga tgcgacgaca
     5581 accaccgcca gccgcgattt cattgggaa
//
LOCUS       DASWIY010000252        21314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3134977, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000252 DASWIY010000000
VERSION     DASWIY010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 21314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21314
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3134977"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..935
                     /locus_tag="VGA59_09270"
     CDS             54..935
                     /locus_tag="VGA59_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX9719909.1"
                     /translation="MELSKDQLEEFEREGYLFFPGRFTAAEMKSLTDAVPELYSRREA
                     YNVREKGKDAVRTNFAAHMYSTPFAKLARHPRMVQPVMDLFDEHAYMHQFKINGKAAF
                     DGDVWQWHQDYGTWLNDDLMPTERAMNVAIFLDDVNEFNGPLMFIPGSHKKGVVEAKH
                     DLTTTSYPLWTVDNELIARLVERAGGKNGGIVSPKGPAGSMILFHSCLVHASGSNLSP
                     WNRVSVYLSLCAVSNHIRRFKRPEYIAHRDFTPIECLPDDCLVKDYPVDLPWRDGVPA
                     SALQTSSVPFDEMRKAA"
     gene            1118..2476
                     /locus_tag="VGA59_09275"
     CDS             1118..2476
                     /locus_tag="VGA59_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9719910.1"
                     /translation="MSLFTKLQQRAAQGKPIRIGLIGAGKFGSMYLAQIPRTPGVHLA
                     GIADLSPDGARANLARVGWKAEQSQAASLDAALDAGTTHIGEDWRALVRHPAIDVIVE
                     CTGHPISAVDHCLEAFANGKHVVNVTVEADAFCGPLLARKAAEAGVVYSLAFGDQPAL
                     ICDLVDWARTCGFPVVAAGRGHKWLPHFCESTPETVWGYYGLTPEQAQRGGLNPKMFN
                     SFLDGSKPSIECTAVANATGLGVPSNGLLYPPASVEDIPYVTRPICEGGVLERKGMVE
                     VISSLEPDGRKIPYDIRMGVWVTVEAETEYIRNCFEEYDAHTDPSGRYFTLYKRWHLI
                     GLEVGVSVASVALRGEPTGAATCWNADVVATAKRDLAPGELLDGEGGYTVWGKLMPAA
                     KSVQVGGLPLGLAHNVKLARAVQKGQSLCWADVAMDTSTHAYKVRKEMEAMFAAPLLK
                     AA"
     gene            2505..3404
                     /locus_tag="VGA59_09280"
     CDS             2505..3404
                     /locus_tag="VGA59_09280"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9719911.1"
                     /translation="MGQHHLPGAAVNIAVLGIGYMGLPMARRLCEAGHVVHVWNRTRE
                     KAQRLAASGAVVHDTAASAVANAEIVISMLQDGSAVGDVLFEHGAARAMKRGALLIDM
                     SAIQPREARDHAARLADFGVSHLDAPVSGGTSGAEAGTLVIMVGGKAADFQHAMPVFG
                     VFGRATHVGPHGTGQLTKLANQMIVGITIGAVAEALLLCQKGGADMAKVKQAISGGFA
                     DSRILQVHGQRMIERNFAPVGRMSVQLKDIQNALATAEEIGFDAPVTALFEKLYADGV
                     EHGLADLETAGLFVELASRNGMQ"
     gene            3499..3574
                     /locus_tag="VGA59_09285"
     tRNA            3499..3574
                     /locus_tag="VGA59_09285"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3532..3534,aa:Ala,seq:cgc)
     gene            3597..3672
                     /locus_tag="VGA59_09290"
     tRNA            3597..3672
                     /locus_tag="VGA59_09290"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3630..3632,aa:Lys,seq:ctt)
     gene            4279..4557
                     /locus_tag="VGA59_09295"
     CDS             4279..4557
                     /locus_tag="VGA59_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719912.1"
                     /translation="MTASVSYTNHPGNRYQKARAELAEHNNRFTLKLLARPLERAATA
                     VWQALMRFAVAYERTAQERIMRELALSHPDLVAQIREARAIAAAEAAH"
     gene            complement(4805..4999)
                     /locus_tag="VGA59_09300"
     CDS             complement(4805..4999)
                     /locus_tag="VGA59_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719913.1"
                     /translation="MIRAIAFVAILAALLFVQFIGAAHQSTAGRAVEGAKPEATFPTA
                     LESDQRALEELAAWPMAAGR"
     gene            5105..6847
                     /locus_tag="VGA59_09305"
     CDS             5105..6847
                     /locus_tag="VGA59_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9719914.1"
                     /translation="MAETSLNATPAAPAPGEAPRVRFGPFVLDRQRAELMRGGIAVPL
                     RPKAFTLLSLFAARPSRVLSKEELLDAAWPGVVVTEDSLTQCVHELRTALGDAGPVML
                     RTVPRRGYRLDAEVIPDTAPSAPDLSAASVPPGSAAPRASGWRERLLGWFGAALTLAL
                     MAGLAIFAVLRPPAQPPLGVSARPPLSIIVLPLVNARGESDWFSDALSNDLTFELGQV
                     PGAFVISRDTAFTYKGKVVDPRAAARDLGVRYVVQGSVQRESERVRLRLSMVDGETGA
                     LQWSQEFDSDRAGLPASLADTAQQVARSLSVQMYRSTGARAAALSPDQVQADDLAMQG
                     WAVWFRGFTPENLREAGRLFDAAVRRDPGSIRGWGGVAVVNGTGAAINWVPDKAAAMT
                     RLQQASDRLQVLDADDFLTYLARSNIANLNGDNEGRLLIGTAMIERFPSHPQSHFTRA
                     QALANLGRFDECVEPAKRAVRLGPRDYFAGIWNWQIGTCQFMRGDYRQAAQFARAAQQ
                     TGPRLPLPPLLLAASLARDGDTNQARRVVADYRARFPDYKTSEIELIMRSRHPAYVEG
                     RTRMIETLRELGMP"
     gene            complement(6873..7316)
                     /locus_tag="VGA59_09310"
     CDS             complement(6873..7316)
                     /locus_tag="VGA59_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719915.1"
                     /translation="MQKHIIAFTIALATGFAGSAFAADAMTKDAYKQAKDKIEEQAKA
                     DKKACEPMKENARDICQAEAKAKAKIAKAELDAQNRPGAKADEKVRLMKAEGEYEVVK
                     EKCEDQKGTAMASCKKDAKVAYDKAKAEARQAKGKAETTNAAKKS"
     gene            complement(7472..8080)
                     /gene="thrH"
                     /locus_tag="VGA59_09315"
     CDS             complement(7472..8080)
                     /gene="thrH"
                     /locus_tag="VGA59_09315"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HEX9719916.1"
                     /translation="MQLVCLDLEGVLVPEIWIAFSERTGIPELRRTTRDEPDYDKLMK
                     YRLALLAEHKLGLPDIQKVIAAMGPMPGAKAFLDDLRQDFQVIILSDTFYEFAMPLMQ
                     QLGLPTLFCHKLETDALGFVANYHLRMPDQKKESVKRFKELNFKVIAAGDSYNDTAML
                     GEAHAGILFHPPENVIREFPQYPVTMNYAELEAEIRKASARI"
     gene            8306..9931
                     /locus_tag="VGA59_09320"
     CDS             8306..9931
                     /locus_tag="VGA59_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719917.1"
                     /translation="MAACLWPQLASAEKQTVCTITVNSPDEQESFRRFLPASKYQFVE
                     LVERGRPDWLTSACQQRVSCDVLVISGHYGSSGGEFFSDALETREFLPTAELERVSCS
                     DSCPSLFSKLKEVYLFGCNTLDSEARYTASPEIARSLAREGRSRPEAERLARLLSAQH
                     GESSRTRMRMMFKDVPVIYGFSTAAPLGPEAASTLNRYFQANGAKDVGNGRASSRLLS
                     SFAPHGMTVARGMTDADPHAAVRQEMCQFADDRLSDVQKLRFVHQLLQRPTAEARAFL
                     DRIERYVAGLDASERQAPEVAQALDAIAHDAGARGRFLTFARDADRPDVRARMLNLAH
                     ELGWLSADQRRDELVRMVGGMLANDTIAGPEVALVCTLNKKHDLDGALGQVASPAGRV
                     DDVAHAAVRACMGSAEGHARTLQGLVSPADADVRIAQTYLRHRPIADVGELRAVVAAI
                     ARMNGSEAQVRALDALAQHYLSDRESLDMLARLYSQTPSWPVQNAIAGILIRSDTKAI
                     ASQDLARTLRERRRKPPSGDSMVDALLSRLQLP"
     gene            10048..11718
                     /locus_tag="VGA59_09325"
     CDS             10048..11718
                     /locus_tag="VGA59_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719918.1"
                     /translation="MPLSLSALRKFAVLAACLAAAWSFGANAAPKTVCTITVNSPDEK
                     ETFARHLPPGEYRFVELVERGRPDWLASACQQRISCDALIISGHFDGGTEFYTDRLDN
                     SEYLSVEEMERASCSDSCPGVFSQLKEVYLFGCNTLKPEPRHSASAEIVRSLLRSGHS
                     PADAVRQSGLLSERHAESNSDRMRNIFRNVPVLYGFSSKAPLGRSARPLLDRYFQSAP
                     PGEIGGGRVSAKLLDLFGPSSMTVAAGLTDADPQAGLRGEVCRFVDDRLSDAQKVGFI
                     HELLQRDMAEVRMFLHHIERYSASIGPAQRLAPKTAAALAAIAGDRAARERYLEFARD
                     ADQPEVRTRMMALAGSLGWLSPAQERAEFMRMIADQIARDRVGRDEVELACARNRESE
                     PDPAVQRLAAGTAQPGKVAQAAVLACLGSAEAHARVVRALTSPNADDVAIAQAYLRHR
                     PLADVGELRTVASAIGRMPTPDAQVRALDTLAQQRLADPESLREIVRLFPLARSVDVQ
                     RAIAGILIRSDYRVLGRSDLARSLRQHRLKSPDGNDVIDALIRVLQAS"
     gene            11841..14471
                     /locus_tag="VGA59_09330"
     CDS             11841..14471
                     /locus_tag="VGA59_09330"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX9719919.1"
                     /translation="MRLVNHSFYVGPNVWFTASGLMLVIEWTREDAAFLKWKPSPAEA
                     ARMLTFLQAALAVPAEAAQSWRGETICDAAAPAIELLLSTAKVLVGDFCVKPAKGRLV
                     DVVPQHLRLFLPCDEPGVGLPAWLLAMEACCPRIGAPGADTAGGLEELRDRYWLFRQA
                     AHRQALNQSTIALARAACDAGIPHYRIATPGQFLQLGQGCFRQRVSETVTDRTSGLAR
                     WLSNDKFATSSFLSSQQIPTSQPQAVASAEQAVQALRRLNLPVVVKPRAAGKGRGVSV
                     NLSSESDVVAAFEHAASFKTGVIVERCVEGDDHRLLAVGGRFIAAARRIPAHVTGDGQ
                     LTVRQLVDKLNQDPRRGMPFDRLLEWVKLDEEAVSVLAADGLALDSVPAQGRLVPLRR
                     SANLSRGGTSVDVTDLVHPDNRALAERVAQLIGLDVTGIDFLTPDIGKSWRDVPCAIL
                     EVNASPGLRPHFAANPHRDVVRPIIDMLYPQGTASRVPTAGITGSAGKTTTCRMVAAI
                     LAQDGKTVALSTTQGTYIGADQLKSGDFAGGASAAALLRDPRVQAGVFELARGGLLKR
                     GMAIDSCDVGAVLNVYDNHVGLNGVRNTEDLARIKRLVPENSRKMAVLNADDPLCLAM
                     RAHLSAPVCLVTEDRDNPVVLEHRLAGGTAAVLDAQRQAPVLKLYAGQQLVGELAAAA
                     IPATWGGAFRPTAINALYAMAIAHGMGVEFTVIAAALAKFESTPDSNPGRMNVYDHLP
                     YRVIVTWADGRRPVAELARFVQTQKVSGTKRLLLCAVGNRPDDFLLDMGAAAASAFGH
                     YVVSDWEDLRGRPAGATAALLAQGLTGAGVPPERVAVAADYYDGLSTAFESAAPGDLL
                     VVVSYASDKSQRAINMRVGA"
     gene            14543..15817
                     /locus_tag="VGA59_09335"
     CDS             14543..15817
                     /locus_tag="VGA59_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX9719920.1"
                     /translation="MNPDADQLWRAPRWALAVLLALLGMLGPFSIDTYIPAFSGIARA
                     LDATPVQMQQTLSAYLFGFAFMNLFHGALADSFGRRPVILWGIAVFALASAGCALSQS
                     IGQLVFFRGLQGLSTGAGIVVSRAVIRDMFPPAQAQQVMSQVTIYFGVAPAIAPIIGG
                     WLFVHAGWHSIFWFLTGVGVVLWMANFRLLPETLHVTQRQPFNVRNLMQGYWQLGASP
                     RFLLLALASGVPFNGMFLYVLAAPAFLGEHLGLAPTEFFWFFVLTIAGIMGGAWVSGR
                     LAGKIPPRRQIRHGFLIMVVVSLVNVAANFMLAPSAAWALLPIAVFAFGWAMMVPVVT
                     LLVLDLHPERRGLASSLQAVIGSTANGLVAGVIAPLVMHSTRALALTSLLMMSVGLAS
                     WFLLHRRWPEIGRSHAVDAINPQSMDPGSSPG"
     gene            complement(15981..17762)
                     /locus_tag="VGA59_09340"
     CDS             complement(15981..17762)
                     /locus_tag="VGA59_09340"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9719921.1"
                     /translation="MTDTFEVQGNFAPAEFSDNPVGAMAQSLPLAPNGFLKMGLAPEL
                     IRAVEDLGFTQPTPVQDEVIPLALPSDDDGAAFIDLMVSSQTGSGKTAAFLLPVLHTL
                     LKQQAAAEARERADYERAAAEAAAKGEPAPKRPKRKDPTNARNFKAATPGALILCPTR
                     ELAQQVAHDAIDLVKHCRGLRVANVVGGMPYQMQIAKLQNADLVVATPGRLLDLQRSM
                     QIKLEQVQFLVVDEGDRMLDLGFADDLAEVNQLTLRRKQTMMFSATFAPRIQQLATRV
                     MRQPRRIEIDNPREKHVNIKQVLFWADNAQHKRKMLDHWLRDTTINQAIVFASTQVEC
                     DGLANDLQQAGFDAVALHGALNQGLRNRRLMALRQGHVQVLVATDVAARGIDVPTITH
                     IFNYGLPMKAEDYTHRIGRTGRAGRDGLAVTFAEFRDRRKIFDIEGYTRQPFKADVIP
                     GLEPQQRAPQGGRGDFGGRVRETPSRDRKFGAGRSGSGFGGGFPDKSVRGPRPFGGGH
                     HNAGFGGHGTDDRGRDGRNGARDGFSYERKGGFNDRFAGERSDGFAARGDFAERSPAF
                     AKPAKPGNGGKVFVPRDIKKRPYKRAN"
     gene            complement(17990..18598)
                     /gene="orn"
                     /locus_tag="VGA59_09345"
     CDS             complement(17990..18598)
                     /gene="orn"
                     /locus_tag="VGA59_09345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX9719922.1"
                     /translation="MSEIVPTVAPSLPKSDQNLIWIDCEMTGLDPEVDRLLEIAVIAT
                     GPSLEPRIEGPVLVIHQCIEQLDRMDSWNKGTHGRSGLIEKVKASFTTEAQAEQEILE
                     FVARYAPKASTPMCGNSISQDRRFLLKYMPRLEAFFHYRNVDVSTLKELAKRWRPDVY
                     NSFKKQQKHTALADVHESIDELVHYREQFLRLESPPALAGKP"
     gene            18636..19904
                     /locus_tag="VGA59_09350"
     CDS             18636..19904
                     /locus_tag="VGA59_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX9719923.1"
                     /translation="MTASHTLTLAFAAFLVAGLAVRFWLASRQIRHVARHREAVPPAF
                     AATVPLAAHQKAADYTIVKTRFGLLELAFGAAILLGWTLLGGLDALNQALIGWLGSGM
                     AQQLALIVGFALISGMLDLPFTLYQTFVIEERFGFNKMSFKLWLADLAKSTVLGAAIG
                     LPIAALILWLMGATGSFWWLWAWAVWMGFNLLLLVIYPTFIAPLFNKFKPLEDESLKQ
                     RVTELMRRCGFAAKGLFVMDGSKRSAHANAYFTGFGAAKRVVFYDTLLSKLSPGEVDA
                     VLAHELGHFKHKHIIKRIAGMFALSLAGFALLGWCSTQAWFYTGLGVRPNLTGPNDAL
                     ALLLFMLAVPVFSFFVSPLLALLSRKHEFEADAYAVQQTSGRDLATALLKLYEDNAST
                     LTPDPVFVKFYYSHPPASERLARMSPGSAA"
     gene            19901..20236
                     /locus_tag="VGA59_09355"
     CDS             19901..20236
                     /locus_tag="VGA59_09355"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX9719924.1"
                     /translation="MTTMLKKKDWSALPRRALTASEIVARLAKAPEWKLTGDGPDVAI
                     EKTYTFANYYETISFVNAVAFIANALDHHPDLSVHYSRCVVRFNTHDVKGLSETDFEC
                     AAQVDALLA"
     gene            20249..21166
                     /gene="rsgA"
                     /locus_tag="VGA59_09360"
     CDS             20249..21166
                     /gene="rsgA"
                     /locus_tag="VGA59_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX9719925.1"
                     /translation="MVERAALDPGLVVASHGRHFLVETPEGERLICHPRGKKSDAVVG
                     DRILWQGTHDEGTIEKIEERRNLFYRQDEIRTKSFAANLDQVLILIAAQPEFSESQLA
                     RALIAAEAERITPIIALNKSDLVEPFERAWKRLLPYQRMRYGVLPLSLKLSGRVDREH
                     LIKHLAGKTTLVLGPSGAGKSTLINLLAPGSLAQTGEISQALNAGKHTTTSTTLYWVD
                     KDRETALIDSPGFQEFGLNHIEPMQLAGCMPDFKAHMGNCKFYNCTHLHEPGCGVIAA
                     IGAGEEGITRSRYRIYQELFAELSQPPRF"
BASE COUNT         3472 a         6934 c         7045 g         3863 t
ORIGIN      
        1 tgaagaccag tgcatgagca accgctttcc acccggtaaa ggagaccatg aacatggaac
       61 tgtcgaaaga tcaactcgaa gagttcgagc gcgaaggcta cctgtttttc cccggccggt
      121 tcacggccgc tgaaatgaag tcgttgaccg acgcggtgcc cgagctgtat agccggcgcg
      181 aggcctacaa cgtccgcgaa aaaggcaagg atgcggtgcg aaccaacttc gcggcgcaca
      241 tgtacagcac gcctttcgcg aagctggccc gccatccgcg catggtgcaa ccggtgatgg
      301 acctgttcga cgagcatgcg tacatgcacc agttcaagat caacggcaag gcagctttcg
      361 acggcgacgt ctggcagtgg caccaggact acggcacctg gctgaacgac gacctgatgc
      421 ctaccgagcg ggccatgaac gtcgcaatct tcctggacga cgtgaatgaa ttcaacggcc
      481 cgctgatgtt cattcccggc agccacaaga aaggcgtggt cgaggcgaag cacgacctca
      541 ccaccaccag ctatccgctt tggacggtgg acaacgagct catcgccagg ctggtggagc
      601 gcgcgggcgg gaagaacggt ggcatcgtct cgcccaaggg cccggccgga tcgatgatcc
      661 tgttccacag ctgcctggtg catgcgtcgg gcagcaacct gtcgccgtgg aatcgggtga
      721 gcgtgtacct gagcctgtgc gccgtctcca accacatccg ccgcttcaag cgtcccgaat
      781 acatcgcgca ccgcgacttc acgccgatcg aatgcctgcc cgacgattgc ctggtgaaag
      841 attacccggt ggatttgccg tggcgcgatg gcgtgcccgc cagcgcgctg cagacgagca
      901 gcgtgccgtt cgatgaaatg aggaaggcag catgagccaa ggctgtcatg ccggacttga
      961 cccgggatcc gtgaaaaatg gttgtcatgc cggacttgat ctaaaacggc tgtcatgccg
     1021 gactcgatcc ggcatccatg actggcatcc atgactcctg gattgcgggt cgagcccgca
     1081 atgacacgtt ccttttcgca atgacaggat tatcctcatg agcctcttca ccaaactcca
     1141 gcagcgcgca gcgcagggca aacctatccg catcggcctc atcggggcgg gcaagttcgg
     1201 ctccatgtac ctcgcgcaga tcccgcgcac acccggcgtg caccttgccg ggatcgcgga
     1261 cctgtcgccc gatggcgccc gcgccaacct cgcgcgcgtg ggctggaagg cggagcaatc
     1321 gcaggcagct tcgctcgacg ccgcgctcga tgccggcacc acccacatcg gcgaggactg
     1381 gcgcgccctg gtgcggcatc cggcgatcga cgtcatcgtc gaatgcaccg gccacccgat
     1441 ctccgccgtg gaccattgcc tggaagcgtt cgcgaacggc aagcatgtcg tcaacgtcac
     1501 cgtggaggcc gatgcgttct gcggtccgtt gctggcgcgc aaggcggccg aagccggcgt
     1561 ggtctattcg ctggcgttcg gcgaccagcc ggcgctgatc tgcgacctgg tggactgggc
     1621 gcgcacctgc ggctttccgg tggtagcggc ggggcgcggc cacaagtggc tgccgcattt
     1681 ctgcgagtcc acgcccgaga cggtgtgggg ctactatggc ctcacgccgg agcaggcgca
     1741 gcgcggcggg ctcaatccca agatgttcaa cagcttcctg gacggctcca agccctcgat
     1801 cgaatgcacg gccgtggcga acgcgaccgg cctgggtgtt ccttccaacg gcctgctcta
     1861 cccgccggcg agcgtggaag acattccgta cgtgacccgg cccatctgcg agggcggcgt
     1921 gctcgaacgc aagggcatgg tcgaggtgat ctcctcgctg gagcccgatg gccgcaagat
     1981 tccctacgac atccgcatgg gggtctgggt gacggtggaa gccgagaccg agtacatcag
     2041 gaactgcttc gaggaatacg acgcgcacac cgatccgagc gggcgctact tcacgctgta
     2101 caagcgctgg cacctgatcg ggctggaggt cggggtgtcg gtcgcgagcg tggcgctgcg
     2161 cggcgagccg accggcgcgg ccacttgctg gaacgccgat gtggtggcca ccgccaagcg
     2221 cgatctggca ccgggcgaac tgctcgacgg cgaaggcggc tacaccgtgt ggggcaagct
     2281 gatgccggcg gccaagtcgg tccaggtagg cgggctgccg ctgggactgg cgcacaacgt
     2341 gaagttggcg cgggccgtgc agaaaggcca gagcctgtgc tgggccgacg tggccatgga
     2401 cacgtcaacg catgcttaca aagttcgcaa ggaaatggag gcgatgttcg ccgcaccgct
     2461 gttgaaagct gcctgacagc aatccggggc gggctggttc catgatggga cagcaccacc
     2521 taccaggagc agcagtgaac atcgccgtgc tgggcatcgg ctacatgggt ttgccgatgg
     2581 cgcgccgcct gtgcgaggcc ggccatgtcg tccacgtgtg gaaccgcacg cgtgaaaagg
     2641 cgcagcggct cgccgcgtca ggagccgtgg tgcacgacac cgcggcctcg gcagtggcca
     2701 acgccgagat cgtgatcagc atgctgcagg acggcagcgc ggtcggcgac gttctgttcg
     2761 agcacggcgc ggcgcgggcc atgaagcgcg gcgcgctgct catcgacatg tcggcgatcc
     2821 agccgcgtga ggcgcgcgat catgccgcgc gcctggccga cttcggcgtg agccatctcg
     2881 atgcgcccgt gtcgggcgga accagcggcg ccgaggccgg aacgctggtg atcatggtgg
     2941 gcggcaaggc cgccgatttc cagcatgcca tgccggtctt cggtgtgttc gggcgcgcga
     3001 cccatgtcgg gccgcacggg acgggccagc tcaccaagct ggccaaccag atgatcgtcg
     3061 gcatcacgat cggcgccgtg gccgaggcgc tgctgctgtg ccagaagggc ggcgccgaca
     3121 tggccaaggt caagcaggcc atcagcggcg gatttgcgga cagccgcatc ctgcaggtgc
     3181 atgggcagcg catgatcgag cggaacttcg cgccggtcgg ccgcatgtcg gtgcagctca
     3241 aggacatcca gaacgcgctg gccacggccg aggagatcgg gttcgacgcc ccggtgacgg
     3301 cgctgttcga aaagctgtac gccgacggcg tggagcatgg cctcgcggac ctcgagactg
     3361 cgggcttgtt cgtcgagctg gccagccgca acggcatgca gtagcggccg cgtcgcggaa
     3421 gaaacctccc ggcttttcgg cataattgca ggttcaccca aacggggccg cgtgttttgc
     3481 taagatacgc tctgctgtgg gggggtagct cagctgggag agcgtcgcgt tcgcaatgcg
     3541 aaggtcggga gttcgatcct cctcctctcc accacagcct cttttttgtt ttcagtgggc
     3601 tcttagctca gttggtagag cagcggactc ttaatccgta ggtcgagtgt tcgagtcact
     3661 cagggcccac cacctactca gcgccagcgc tggaaagcct gctatcggaa cggtagcagg
     3721 ctttttcctt tcggtcatgg gcgaatttgt tggcgaatag cggctcagcg cagcatcggc
     3781 ttcgcacgag cggctgccgc ccaaattgat gttcacggtg cgcgtgacgt tcgccgggat
     3841 tgccgcagta ccaacggccg gtccttgccc atattttgtt cggctcggca agaatgcgca
     3901 gagtgttcct caggccgccg atgcccgtgt cgttggtcta cctgcctggt cgccagcact
     3961 cgccccgcat tcgctggttc ttgcagacgc tgcaggaaat gatcccgtca acggcgccat
     4021 gggaggacat aacgcggacg taggccatcc acctccagcc cttgctattg gatgcacacg
     4081 cgtgcggcat tacttgcgcc cgaacaacaa gttccaagtc ttttctggcc tgcgcgaggt
     4141 agccagccgg tcagcgcaga catcaccgtg ctaaatgtct ttcatcacta atcaaaattg
     4201 gcggaatatc agtaaaaacc ctaatatctc aaacatccgc tcagcaatcc gccgggcgct
     4261 acgaaaggga ccaaagaaat gaccgccagc gtcagctaca ccaaccatcc tggcaaccgc
     4321 taccagaagg cgcgcgccga gcttgccgag cacaacaacc ggttcacgct caagctcttg
     4381 gcaaggcccc tggagcgtgc ggcaacagcc gtctggcagg cgcttatgcg cttcgccgtc
     4441 gcctacgagc gtaccgctca ggagcgcatc atgcgggaac ttgccctgag ccaccccgac
     4501 ctggtggcgc agatccgcga agcgcgggcc atcgccgctg ctgaagcggc ccattagtca
     4561 ggagtgagct tcctgaacgg cggccgtcgc cgccgctcaa cccaggtgag attgtccccg
     4621 ccggcgtgcc gcctggtagc cgcacggtag ccgtcgtgtc ccgcgaggcg cgaccgattg
     4681 tccccgcccc gtctccggcg acagggcggg cgtgtcgtct ctggaaagcc tgctatcaga
     4741 acggtagcag gctttttctt tgttcagctg ggtgtatcac tgcggtcgcg tgagccggcg
     4801 aacactagcg cccggcagcc attggccagg ccgccagttc ttcgagggca cgttgatcgg
     4861 actcgagggc cgtggggaag gttgcttctg gcttggcgcc ttcgaccgcg cggccggcag
     4921 tggactggtg cgcggcgcca ataaactgta caaacagaag cgccgcgaga atggcgacga
     4981 atgcgatggc acggatcatg acgaactcct tgtcgattaa gttcagccgg cggcgggatt
     5041 gccgcgtggc cgcctgacca ctctgggtga tggacgtgga taagtcctta acatgcgctg
     5101 aacgatggct gaaacttcgc tgaatgcgac ccctgcggcg ccagcgccag gggaggcgcc
     5161 gcgggtgcgc ttcggtccct tcgtgctgga ccgtcagcgc gccgagctga tgcggggcgg
     5221 catcgcggtg ccattgcggc ctaaggcctt cacactgctg agcctgttcg cggcgcgccc
     5281 cagccgcgtg ctctccaagg aggagctact cgacgcggcc tggccaggtg tggtagtcac
     5341 cgaggattcg ctgacacagt gcgtgcatga gctgcggacc gcgctcggcg atgcaggacc
     5401 ggtgatgctg cgcacggtcc ctcgccgcgg ctaccgcttg gatgccgagg tgataccgga
     5461 caccgcgccc tcagcccctg atctttccgc ggcctccgta ccgcctggct ctgccgcacc
     5521 gcgtgcctcc ggctggcgcg aacggctgct gggctggttc ggtgccgcgc tcacgctcgc
     5581 gttgatggct ggattggcca tctttgccgt gctgcgcccg ccggcgcagc cgccgctggg
     5641 cgtgtccgcg cgcccgccgc tttcaatcat cgtgctaccg cttgtcaacg cccgaggcga
     5701 gtcggactgg ttcagcgacg cactgtccaa cgatctgacg ttcgagctcg ggcaggtccc
     5761 cggcgccttt gtgatctctc gcgacaccgc gttcacctac aagggcaagg tggtggaccc
     5821 gcgtgcagcc gcgcgagacc tcggtgttcg ctacgtggtg cagggctcgg ttcaacgcga
     5881 gagcgagcgc gttcgcctgc ggctgtcaat ggtggacggc gaaacggggg ctctgcagtg
     5941 gtcgcaagag ttcgacagcg acagggctgg gcttccggcg tcgctcgcgg acacggctca
     6001 gcaggtggcg cgcagcttga gtgttcagat gtaccgctcg accggcgcaa gggcagccgc
     6061 gctgagcccc gaccaggtgc aagcggacga cctcgcgatg cagggctggg ccgtgtggtt
     6121 tcgcggcttc acacccgaga acctgcgcga agccggccgc ctgttcgacg cggccgtgcg
     6181 ccgcgatccg ggctcgatcc gcggctgggg tggcgtggca gtcgtcaacg ggaccggcgc
     6241 ggccatcaac tgggtgccgg acaaggcggc ggccatgacc cgcctccagc aggcgtccga
     6301 tcgcttgcag gtgctcgacg ccgacgactt cctcacctat ctggcgcgtt ccaacatcgc
     6361 gaacctcaat ggcgacaacg aggggcggct tctgattggg acggcgatga tcgagcgttt
     6421 tccgagccat ccgcaatcgc atttcacccg cgcccaggcg ttggcgaacc ttgggcgctt
     6481 cgacgaatgc gtggaaccgg cgaagcgcgc cgtccggctc gggccgcggg actatttcgc
     6541 cggcatctgg aactggcaga tcggcacctg tcaattcatg cgcggcgact accgccaggc
     6601 cgcgcagttc gcccgcgccg cgcagcaaac gggcccgaga ctgccgttgc ctccgctgct
     6661 gctcgccgcg tcgctggcgc gcgatggcga caccaatcaa gccagacgcg tcgttgccga
     6721 ctacagggcg cggttccccg actacaagac ttcggagatc gagctgatca tgcgcagccg
     6781 ccatccggcc tacgtcgagg ggcgcacccg catgatcgaa acgttgcgtg aactcgggat
     6841 gccctgaatg ggtgctcgag ccaaaggaga tgtcaggact tcttcgcggc gtttgtcgtt
     6901 tcggctttgc ccttggcctg ccgggcctcg gccttggcct tgtcgtaggc gaccttcgcg
     6961 tccttcttgc atgaagccat ggccgtgccc ttctggtctt cgcacttctc cttaacgacc
     7021 tcgtactcgc cctctgcctt catcagccga accttctcgt cggccttggc gccggggcga
     7081 ttctgcgcat caagctcggc cttggcgatc ttcgctttgg ccttggcctc ggcctggcag
     7141 atgtcccttg cgttctcctt cataggctcg caagccttct tgtcggcctt ggcctgttcc
     7201 tcgatcttgt ccttggcctg cttgtacgcg tccttggtca tggcgtcggc cgcgaaggcg
     7261 ctgccggcga agccagtggc cagcgcaatg gtgaaggcaa tgatgtgttt ctgcatgacg
     7321 tttcctttct ctgcaaagtg cggatgcaca tccatcaacg tatggcgcgg cagcacggtc
     7381 cgtcggcgtt gttcggtttc agttgtcgga aagcgactac agcttcatgg ctgagcgtct
     7441 tctcaaccag tgtagggatg ggcctgcggc ctcagatgcg ggcgctggct ttgcgaatct
     7501 cggcttcaag ttccgcatag ttcatggtca ccgggtattg cggaaattcg cggatcacgt
     7561 tctcaggcgg atggaacagg atgccggcgt gggcttcgcc caacatggcg gtgtcgttgt
     7621 aggaatcgcc ggcggcgatg accttgaagt tgagttcctt gaagcgcttc accgattcct
     7681 tcttctggtc cggcatgcgc aggtggtagt tggccacgaa gccgagcgca tcggtctcca
     7741 acttgtggca gaacagcgtc ggcaggccga gctgctgcat cagcggcatg gcgaattcgt
     7801 agaaggtgtc ggagaggatg atcacctgga agtcctggcg caggtcgtcg agaaaggcct
     7861 tggcgccggg catcggaccc atcgccgcga tcaccttctg gatgtcgggc aggcccagct
     7921 tgtgctcggc cagcagggcc aggcgatact tcatcagctt gtcgtaatcc ggctcgtcgc
     7981 gcgtggtgcg gcgcaattcg ggaatgccgg tgcgctccga gaacgcgatc cagatttcag
     8041 gaacgaggac gccctcgagg tcgaggcaga cgagttgcac ggattgctcc ctggcggttg
     8101 agtaacggcg gcgattttac gcgaagcccc gttttcgccg gaccgccaag cctgattacg
     8161 gggagcgagc cgacacaggg cgaccgcatt cgcctacaaa cacgcacagg cgacagcgat
     8221 caaattcaat ggccgcgtca ttctcgcgtc acagggtccg aacgtcatgc gcttgcccgc
     8281 gagccttctt cacttgattt gcctattggc agcatgcctg tggccgcagc tcgcgtcggc
     8341 cgaaaagcag acggtgtgca ccatcaccgt caattccccc gacgaacagg aatcgttccg
     8401 ccgcttcctg cccgcttcga agtaccagtt tgtcgagctg gtggaacgcg gtcggccgga
     8461 ttggctgact tccgcctgcc agcagcgcgt ttcctgcgat gtgctcgtga tctccggcca
     8521 ctacggctcc tccggcggcg agttcttttc ggacgcgctg gagacgcgcg aattcctgcc
     8581 caccgccgaa ctcgagcgcg tttcctgctc ggattcctgc ccgagcctgt tttccaagct
     8641 gaaggaggtc tacctcttcg gctgcaacac gttggattcg gaggcgcgat acacggcatc
     8701 gccggagatc gcgcgcagcc tggcgcgtga agggcgctcg cgcccggagg cggagcggct
     8761 cgcgcgattg ttgagtgcgc agcacggcga gagcagccgc accaggatgc ggatgatgtt
     8821 caaggacgtt ccggtgattt acggcttttc aacggcagcg ccgctgggtc cggaggccgc
     8881 ctccacgctc aaccgctact tccaggcgaa cggcgcgaaa gacgtgggca acggccgcgc
     8941 cagcagccga ctgctgtcga gcttcgcgcc ccacggcatg acggtggccc ggggcatgac
     9001 cgacgcggat ccgcacgccg ccgtgcggca ggagatgtgc cagttcgccg atgatcggtt
     9061 gtcggacgtg cagaagctgc gcttcgtcca tcagctgctg caaagaccga cggcggaagc
     9121 cagggcgttt ctcgaccgca tcgaaagata cgtggcgggg ctcgacgcga gcgagcggca
     9181 ggcgccggag gtagcgcagg cgctcgatgc catcgcgcac gacgccggcg cccgcggacg
     9241 ctttctcacc ttcgcgcgcg acgccgatcg acccgacgta cgggcgcgca tgctcaatct
     9301 cgcgcacgag ctgggctggt tgtcggccga ccaacggcgc gacgagctgg tgcggatggt
     9361 cggtggcatg ctggccaacg acacgatcgc cggcccggag gtggccctgg tatgcacgct
     9421 gaacaagaag cacgacctcg atggcgcgct tggccaagtc gcctcaccgg cgggccgggt
     9481 cgatgacgtg gcgcacgccg ccgttcgcgc ttgcatgggc agcgccgagg gtcacgcgcg
     9541 cacgctgcaa gggctggtca gcccggccga cgccgatgtc cggatcgccc agacctatct
     9601 tcgtcaccgt ccgatcgccg acgtcggcga actgcgcgcg gtggtcgcgg cgatcgcccg
     9661 catgaacggg tccgaggcgc aggtgcgcgc gctggacgcc ttggcgcagc actacctgtc
     9721 cgatcgcgaa agcctggaca tgctggcgag gctctattcg cagacgccat cctggcccgt
     9781 ccagaacgcg atcgcgggca ttctgatccg ctctgacacc aaggcgatcg ccagccagga
     9841 tctcgcgcgt acgctgcgcg agcgccgccg caagcccccc tcgggcgaca gcatggtcga
     9901 tgcgctcttg agtcggctgc aattgcctta gcacccggca gagcctgagc gaactttcac
     9961 tgcgttacag cgttacatat cgctgcgcag cgcagagctt gtgcaatact atggcgccgc
    10021 accaagccgc gtcgagtcgt cctgcccatg cccttgtccc tgtccgcttt gcgcaaattt
    10081 gccgtcctcg ccgcgtgcct cgctgccgcc tggtcgttcg gtgcgaacgc cgcgcccaag
    10141 acggtgtgca cgatcaccgt gaactcgccg gatgagaagg agaccttcgc gcggcatctg
    10201 ccgcctggcg aataccggtt cgtcgagttg gtggagcgcg ggcggccgga ctggctggcc
    10261 tccgcctgcc agcagcgcat ttcctgcgac gcgctcatca tttccggcca tttcgacggc
    10321 ggcacggagt tctataccga tcgcctcgac aacagcgagt acctgtcggt cgaggagatg
    10381 gagcgcgctt cgtgcagcga ctcctgtccc ggcgtgtttt cgcagctcaa ggaagtctat
    10441 ctgttcggct gcaacacctt gaaaccggag ccccggcata gcgcctcggc ggagatcgtg
    10501 cgcagcctcc ttcgctcggg ccactccccc gcggacgccg tgcggcaatc gggactgttg
    10561 agcgagcggc atgccgaaag caatagcgac cgcatgcgga acatcttcag gaacgtgccg
    10621 gtactctacg gtttttcgtc gaaagcgccg ctgggacgct cggcgcggcc gctgctcgat
    10681 cgctattttc aatcggcccc cccaggcgag atcgggggcg ggcgtgtgag cgccaagctc
    10741 ctggacctct tcgggccaag ctccatgaca gttgccgccg gcttgaccga tgcggatccg
    10801 caagcgggct tgcgcggcga agtctgccgc ttcgtggacg atcggctctc ggacgcgcag
    10861 aaggtcggct tcattcacga gctgctgcag cgcgatatgg ccgaagtgcg gatgttcctt
    10921 catcacatcg agcgctactc ggcctccatt ggaccggcgc agcgcctcgc gccgaagacg
    10981 gccgcggctc tcgctgcgat cgccggcgac cgcgccgcgc gcgaacgata cctcgagttt
    11041 gcgcgcgacg ccgaccaacc ggaggttcgt acccgaatga tggcgctggc gggcagcctt
    11101 gggtggctgt cgcccgcgca ggagcgtgcg gaattcatgc gcatgatcgc ggaccagatc
    11161 gcccgcgatc gcgtgggcag ggatgaggtg gagctcgctt gcgcgcgcaa ccgggaaagc
    11221 gagccggacc ccgcggtgca acggttggcc gccggcacgg cgcagcccgg caaggtggcg
    11281 caggccgcgg tgctggcatg cctgggaagc gccgaggcgc atgcgcgcgt cgttcgtgca
    11341 ctgacgagtc cgaacgccga cgatgtcgcg atcgcgcagg cctacctgcg acaccggccg
    11401 ctggccgacg tcggcgaatt gcgcaccgtc gcatcggcca tcggacgcat gcccacgccc
    11461 gacgcgcagg tgcgcgccct cgacacgctg gctcagcaac gcctggccga cccggaaagc
    11521 ctgcgggaaa tcgtccgcct cttcccgctg gcccggtcgg tcgatgtgca gcgcgcgatc
    11581 gccggtattc tgatccgttc cgactatcgg gtgctggggc ggtccgacct tgcgcgctcg
    11641 ctgcggcagc accgcctcaa gtccccggac ggcaacgacg tgatcgacgc gctgattcgc
    11701 gtgctccagg cgagctaggg cgtctgccgt cactctcgac gggcggcctg tcttcaggct
    11761 ttgtcatact ccaggcgttg cgtgtcgccg ttcctttttc attggcgaat ggccgcatcg
    11821 cgatcggagc aagccaggac atgagactcg tcaatcacag cttctatgtc ggtccaaatg
    11881 tctggttcac ggcttccggg ctgatgctgg tcatcgaatg gacgcgggaa gatgcggctt
    11941 ttctcaagtg gaagccaagc cccgccgagg cggcgcggat gttgacgttc ctgcaggcgg
    12001 cgcttgccgt cccggcggaa gcggcgcaaa gctggcgtgg cgaaacgatc tgcgatgcgg
    12061 cggcccctgc catcgaactg ctgttgtcca ccgccaaggt cctggtgggc gatttttgcg
    12121 tgaaaccggc caagggtcgg ctggtggatg tcgtgcccca gcacctgcgt ttgttcctgc
    12181 catgcgatga acccggcgtc ggtttgcctg catggctact ggcgatggaa gcctgctgcc
    12241 cgcgcattgg cgctcccggc gccgacaccg cgggcggact cgaagaactg cgcgatcgtt
    12301 attggctttt tcgccaggcg gcgcaccgcc aagcactcaa ccagagcacc atcgccttgg
    12361 ccagggctgc ctgcgacgcc ggcatcccgc attaccgcat agcgacaccg ggtcagttcc
    12421 tgcagctggg ccagggctgc tttcggcaaa gagtcagcga gacggtcacg gaccgcacga
    12481 gcggcttggc gcgctggctg tccaacgaca agttcgcgac gtcttccttc ctgtcctcgc
    12541 aacagatccc gacttcccag ccacaggccg tggcttccgc ggagcaggct gtccaggccc
    12601 ttcgacgctt gaacctgcct gtcgtcgtca agccgagggc ggcgggcaag ggccggggcg
    12661 tcagcgtgaa cctgtccagt gagagcgatg tcgtcgctgc tttcgaacac gcagcaagtt
    12721 tcaagaccgg cgtgatcgtc gagcgttgcg tcgaaggcga cgaccaccgg ttgctggccg
    12781 tgggtggccg gttcatcgcc gcggcccgcc gcattccggc tcacgtgacg ggcgacgggc
    12841 agctcacggt caggcagctg gtcgacaagc tcaaccagga tccgcgccgg ggcatgccgt
    12901 tcgaccgcct gctggagtgg gtgaaactcg acgaggaagc ggtgtccgtg cttgccgcgg
    12961 acggcctcgc cctcgactcg gttcccgcgc aaggccggct ggttcccctc aggcggtccg
    13021 ccaacctctc ccggggcggc acttcggtgg acgtgaccga tctggttcac cccgacaacc
    13081 gggccctggc ggagcgggtg gcccaactga tcgggctgga cgtgaccggc atcgatttcc
    13141 tcacacccga catcggcaaa tcgtggcggg atgtcccatg cgccatcctg gaggtcaacg
    13201 cgtcgccggg tttgcggccg catttcgcgg ccaatccgca ccgcgacgtg gtgcggccga
    13261 tcatcgacat gctctacccg cagggcacgg ccagccgcgt ccccacggcg ggcatcaccg
    13321 gcagcgcggg caagacgacg acctgccgca tggtggccgc catactggcg caagacggaa
    13381 agaccgtggc gctgtcgacc acccagggga cctacatcgg ggcagaccag ctcaagtccg
    13441 gtgatttcgc gggtggggcc agtgctgccg ccctgctgcg cgacccccgg gtgcaggcgg
    13501 gcgttttcga gctggcccgc gggggccttc tgaagagggg catggccatc gacagctgcg
    13561 atgtcggcgc ggtgctgaac gtctacgaca accacgtcgg gctgaacggc gtgcgcaaca
    13621 cggaggatct cgcgcgcatc aagcgcctgg tgcccgaaaa ctcgcggaag atggccgtcc
    13681 tgaatgcgga cgatcccttg tgcctggcca tgcgcgcgca tctttcggcc ccggtttgcc
    13741 tggtcaccga ggatcgcgac aaccccgtgg tactggaaca tcggctggcg gggggcaccg
    13801 cggcggtcct ggatgcgcag cggcaagcgc cggttctgaa actttacgcg gggcaacagc
    13861 tcgtcggcga gctcgcggcc gcggcgattc cagccacctg gggcggcgct ttccggccca
    13921 ccgcgatcaa cgccctctat gcgatggcga ttgcccacgg catgggcgtc gagttcaccg
    13981 tgatcgcggc cgcactggcc aaattcgagt caacccccga cagcaatccg gggcgcatga
    14041 atgtctacga ccatctgccg tacagggtta tcgtcacctg ggccgacggg cgtcgtcccg
    14101 tggcggaact cgcccgtttc gtgcagaccc agaaggtctc cggcacgaaa cgccttctgc
    14161 tctgcgcagt gggcaacaga cccgacgact tcctgttgga catgggcgcg gcggccgcgt
    14221 ccgcgttcgg gcactatgtc gtttcggatt gggaagatct gcgcggacgc ccggcaggcg
    14281 ccacggctgc gcttctggcg caaggcttga cgggagcggg tgtgccgccg gagcgtgtcg
    14341 ccgttgcggc tgactattac gacgggctca gcacggcatt tgaaagcgcc gccccgggcg
    14401 acctgttggt ggtggtcagt tacgccagcg acaagagcca gcgtgccatc aatatgcgcg
    14461 taggcgcatg agtcccagtg cgggcgcgcg atgcgcctga cggacagcgg ccgggcgacc
    14521 gaggcattag actcgacggc tcgtgaatcc cgatgccgat caactctggc gcgcgccgcg
    14581 ctgggccctc gccgtattgc tcgccctctt gggcatgctg gggccgtttt ccatcgacac
    14641 ctatatcccg gccttctcgg gcatcgcccg ggcgctggac gcgacgccgg tgcagatgca
    14701 gcagacgctg tcggcatacc tgttcggctt tgccttcatg aacctgttcc atggcgcact
    14761 ggccgacagc ttcggccggc ggccggtcat cctctggggc atcgccgtgt tcgcgctcgc
    14821 ctctgccgga tgcgcgttgt cgcagagcat cggccagctg gtgtttttcc gcggcttgca
    14881 gggcctgtcc accggcgcgg gcatcgtggt gtcgcgcgcc gtcatccgcg acatgttccc
    14941 gccggcgcag gcgcagcagg tgatgagcca ggtcaccatc tacttcggcg tggcacccgc
    15001 catcgcgccg atcatcggcg gctggctgtt cgtccatgcc ggctggcaca gcatcttctg
    15061 gtttctcacc ggcgtgggcg tggttctctg gatggcgaat ttccggttgc tgcccgagac
    15121 cctgcacgtg acacagcgcc agcctttcaa tgtgcgaaac ctgatgcagg gctactggca
    15181 gctcggggcc agcccgcgat tcttgctgct ggccctggcc agcggcgtcc cgttcaacgg
    15241 gatgttcctg tatgtgttgg cggcgcccgc cttcctgggc gagcacttgg gcctggcacc
    15301 caccgagttc ttctggttct tcgttcttac gatcgcgggg atcatgggcg gcgcctgggt
    15361 cagcggccgc ctggccggca agattccgcc gcgccggcag atccggcatg gattcctgat
    15421 catggtggtg gtgtcgctgg tcaatgtcgc cgccaacttc atgctcgcgc ccagtgccgc
    15481 gtgggcgctg ctgccgatcg cggtgttcgc gttcggctgg gcaatgatgg tgccggtcgt
    15541 cacgctgctg gtgctcgacc tccatccgga gcgccgcggg ctggcgtcgt cgctgcaggc
    15601 ggtgatcggg tccaccgcca acggtttggt ggccggcgtg atcgcgccgc tggtgatgca
    15661 ctccacgcgc gcgctcgcgc taacgtcgct gctgatgatg agcgtgggcc tggcgtcgtg
    15721 gttcctgttg caccgccgct ggccggagat cggccgctcg cacgcggtgg acgcgatcaa
    15781 tccgcaatcg atggatcccg ggtcaagccc gggatgacaa atccctctgt cattgcgggc
    15841 ttgatccgca atccaggaag tcatggatcc cgggtcaagc ccgggatgac agaccgtgcc
    15901 cgggatgaca gcccgtgccc gggatgacag cccgtgcccg ggatgacagc cgtcgaattg
    15961 gcgcagccgg gaagccgcgg tcagttcgcc cgcttgtacg ggcgcttctt gatgtcgcgc
    16021 ggaacgaaca ccttgccgcc gttgcccggc ttcgccggtt tcgcgaacgc gggactgcgc
    16081 tcggcaaaat cgccgcgcgc ggcaaaaccg tcgctgcgct cgccggcaaa gcggtcgttg
    16141 aagccgccct tgcgctcgta gctgaagcca tcgcgcgcac cgttacggcc gtcacggccc
    16201 ctatcgtcgg tgccatgccc gccgaagccg gcattgtggt gaccgccgcc gaaggggcgc
    16261 ggcccacgca cggacttgtc ggggaagcca ccgccgaagc ccgagccgct gcggccggcg
    16321 ccgaacttgc ggtcgcgtga cggagtctcg cgcacacgac cgccgaagtc gccgcggccg
    16381 ccctggggcg cgcgctgctg cggctccagg ccgggaatca cgtccgcctt gaacggctgc
    16441 cgggtatagc cctcgatgtc gaagatcttg cggcggtcgc ggaattcggc gaacgtgacg
    16501 gccaggccat cacggccggc gcggccggtg cggccgatgc ggtgggtgta gtcctccgcc
    16561 ttcatcggca agccgtaatt gaagatgtgg gtaatcgtcg gcacgtcgat gccgcgggca
    16621 gccacgtcgg tagccaccag cacctgcacg tgaccctggc gcagcgccat caggcggcga
    16681 ttgcgcaggc cctggttcag ggcgccgtgc agcgcgacgg cgtcgaagcc ggcctgctgc
    16741 aggtcgttgg ccagcccgtc gcattccacc tgggtgctgg cgaaaacgat cgcctggttg
    16801 atggtggtgt cgcgcagcca gtggtccagc atcttgcgct tgtgctgggc gttgtcggcc
    16861 cagaacagca cctgcttgat gttgacgtgc ttttcccgcg ggttgtcgat ctcgatcctc
    16921 ctgggctggc gcatgacacg ggtagccagt tgctggatgc gcggcgcgaa cgtggcgctg
    16981 aacatcatgg tctgcttgcg gcgaagcgtg agctggttga cctcggccag gtcgtcggca
    17041 aagccgagat ccagcatgcg gtcgccttcg tccacgacca ggaattgcac ctgttcgagc
    17101 ttgatctgca tcgagcgctg caggtccagc aggcggccgg gcgtggcgac cacgagatcg
    17161 gcgttctgca gcttggcgat ctgcatctgg tagggcatgc cgcccaccac attggcgacg
    17221 cgcaggccgc ggcaatgctt gaccaggtcg atggcgtcgt gcgccacctg ctgcgcgagt
    17281 tcgcgcgtcg ggcacaggat cagcgcgccg ggcgtggcgg ccttgaagtt gcgggcattg
    17341 gtcgggtcct tgcgcttggg gcgcttgggg gccggctcgc ccttggcggc ggcttcggct
    17401 gcggcgcgct cgtagtcggc gcgttccctc gcttccgctg ccgcctgctg cttgagcagc
    17461 gtgtgcagca cgggcagcag gaaggccgcg gtcttgccgc tgccggtctg gctggaaacc
    17521 atcaggtcga tgaatgccgc gccgtcgtca tcggacggca aagccagcgg aatgacttca
    17581 tcctgcaccg gtgtcggctg ggtgaacccc aggtcttcga cggcgcggat cagctcgggc
    17641 gcgaggccca ttttcaggaa gccgttgggc gccaggggca gcgattgcgc catggcgcca
    17701 acggggttgt cggagaattc ggcaggcgca aagttgccct gcacttcaaa agtgtcggtc
    17761 atgagttact cacacgcata cgccaccgag ggtggcgttg tcgtcaatgg tcaaaaaaca
    17821 tcaaccatca aacgactgtc aggcccgaag gctctgctgg ctctgcgctg tcgcgccgtg
    17881 tcaggaattt cggcgccgtg gcgccgggtc gatcctgtcg tatgagccgg tgtgtgaagg
    17941 gagatgcacg agggctgtcc atgcagccga gccagcgatt atcgcacagt cagggtttgc
    18001 ccgccaatgc gggtgggctc tcgagcctca ggaactgctc gcggtagtgc acaagctcgt
    18061 cgatggactc gtgaacatcg gccagggccg tgtgcttctg ctgcttcttg aacgagttgt
    18121 agacgtccgg gcgccagcgc ttggccagct ccttgagcgt gctcacatcg acgttgcggt
    18181 agtgaaagaa cgcttcgagc ctgggcatgt atttcagcag gaagcgccgg tcctggctga
    18241 tgctgttgcc gcacatcggc gtggaggcct tgggtgcgta tcgcgcgaca aactcgagga
    18301 tttcctgttc ggcctgggct tccgtcgtga atgaggcctt gaccttctcg atgaggccgc
    18361 tgcggccgtg cgtgcccttg ttccaggaat ccatcctgtc gagctgttcg atgcattggt
    18421 ggatcaccag caccgggccc tcgatcctcg gctcgaggct gggaccggtt gcgatcacgg
    18481 cgatctcgag cagacggtcg acctcggggt cgagcccggt catttcgcaa tcgatccaga
    18541 tcaggttctg gtcgcttttg ggaaggctgg gagccactgt gggtacgatt tccgacatgc
    18601 gtgcgattgt cgccgatggc ctaaacttcg tcgcaatgac cgcctcccat acgttgaccc
    18661 tggcattcgc cgcttttctg gtcgcaggcc tcgccgtcag gttctggctg gcgtcccgcc
    18721 agatccgcca tgtcgcccgg caccgcgagg cggtaccgcc cgcattcgcc gccaccgttc
    18781 ccctggcggc gcatcagaag gcggcggatt acacgatcgt caagacccgc ttcggcctgc
    18841 tcgagctggc attcggcgcc gccatcctgc tgggctggac gctgctgggc ggcctggacg
    18901 ccttgaacca ggcgctgatc gggtggctgg gcagcggcat ggcacagcag ctggccctga
    18961 tcgtgggctt cgcactcatc agcggcatgc tggatctgcc attcacgctg taccagacct
    19021 ttgtgatcga agaacgcttc ggcttcaaca agatgagctt caagctctgg ctggccgatc
    19081 tcgcgaagtc caccgtcctt ggcgcggcga tcgggctgcc catcgcggca ctgatcctct
    19141 ggctcatggg ggccaccgga agcttctggt ggctctgggc ctgggccgtg tggatgggct
    19201 tcaacctcct gctgctggtg atctatccga ccttcatcgc gccgcttttc aacaagttca
    19261 agccgctcga agacgaatcg ctgaagcagc gcgtcaccga actcatgcgg cgctgcgggt
    19321 tcgcggccaa gggcctgttc gtgatggacg gcagcaagcg cagtgcccat gccaatgcct
    19381 atttcaccgg tttcggcgcg gccaagcgcg tggtgttcta cgacacgctg ctctcgaagc
    19441 tgtcccccgg cgaggtcgac gccgtgctcg cgcacgaact gggccacttc aagcacaagc
    19501 acatcatcaa acgcatcgcc gggatgttcg ctctcagcct ggccggcttc gcattgctgg
    19561 ggtggtgctc gacccaggcc tggttctata cgggcctggg ggtgcggccc aacctcacag
    19621 ggcccaacga cgcgctggcc ctgctgctgt tcatgctggc ggtgccggtg ttttcattct
    19681 tcgtttcgcc gctgctcgcg ctgctgtcgc gcaagcacga gttcgaggcc gacgcctatg
    19741 ccgtgcagca gaccagcggc cgggacctcg ccaccgcgct gctcaagctg tacgaggaca
    19801 atgcctccac gctgaccccc gatccggtct tcgtgaagtt ctattattcg cacccgccgg
    19861 cctccgaacg gctggcacga atgagcccag ggagtgccgc atgacgacga tgctgaaaaa
    19921 gaaagactgg tcggcgttgc cgcgccgcgc gctcacggcc tctgaaatcg ttgcgcggct
    19981 ggccaaggcg ccggaatgga agctcaccgg cgacggcccc gacgtcgcga tcgagaagac
    20041 ctacaccttc gccaactact acgagacgat ctcgttcgtc aacgccgtcg ccttcatcgc
    20101 caacgccctg gaccaccacc ccgacctctc ggtccactac agccgctgtg tcgtgcgatt
    20161 caacacgcac gacgtcaagg gcctgtccga gaccgacttc gaatgcgcgg cgcaagtcga
    20221 cgccctgctg gcatgaggac gccaacgctt ggttgagcgt gccgcgctcg accccgggct
    20281 ggttgtcgcc agccacggcc gccacttcct ggtcgagacg ccggaaggcg agcgcctgat
    20341 ctgccatccg cgcggcaaga aaagcgatgc cgtggtcggc gaccgcatcc tgtggcaggg
    20401 cacgcatgac gagggcacga tcgagaagat cgaggagcgg cgcaacctgt tctaccggca
    20461 ggacgagatc cgcaccaaat ccttcgccgc caacctggac caggtgctga tcctgatcgc
    20521 cgcccagccc gaattctccg aaagccagct tgcacgcgcc ctgatcgcag ccgaagccga
    20581 gcgcatcacg cccatcatcg cgttgaacaa gagcgacctg gtggaaccat tcgagcgcgc
    20641 gtggaaacgc ctgctgccgt accagcgcat gcgctacggc gtactgcccc tgtcgctcaa
    20701 gctttcgggc cgggtcgacc gcgagcacct gatcaagcac ctggccggca agacgacgct
    20761 ggtgctgggc ccctcgggcg ccggcaagag cacgctcatc aacctgctgg cgccgggctc
    20821 gctggcgcag accggagaga tttcgcaggc gctgaacgcg ggcaagcaca cgaccaccag
    20881 caccaccttg tactgggtcg acaaggaccg cgagacggcc ctgatcgatt cgccgggctt
    20941 ccaggaattc ggcctgaacc atatcgagcc gatgcagctg gccgggtgca tgcccgactt
    21001 caaggcgcat atgggcaatt gcaagttcta caactgcacc cacctgcacg agccgggttg
    21061 cggcgtgatc gcggcgatcg gcgctggcga ggagggcatc acgcggtcgc gctaccgcat
    21121 ctatcaggag ctgttcgccg agctgtctca gccgccgcgg ttctagcacg ctcactcaca
    21181 ggatgtcatg ccgagacatc ctaacttcct ggattgcggg gccgggccgg agcgtcatgc
    21241 ctcggtacgt gcatagcgta ggcccggcat tgggctcgct gcactggaca gctgcgcgcc
    21301 ccgggcgttc cgcg
//
LOCUS       DASWIY010000253         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1656730, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000253 DASWIY010000000
VERSION     DASWIY010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656730"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VGA59_09365"
     CDS             complement(<1..761)
                     /locus_tag="VGA59_09365"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX9719926.1"
                     /translation="MNDQLIIFDTTLRDGEQSPGASMTRDEKVRIAKVLERMRVDVIE
                     AGFPVASPGDFEAVKAVAEAVTESTVCGLARAVEKDIDRAAEAIKPAKRGRIHTFIAT
                     SPIHMQHKLRMTPEQVLERAVQMVKRARQYTDDVEFSAEDAVRSDIDFLCRVFEAAIN
                     AGARTINVPDTVGYSIPQVWGERMKTLIERVPNSDKAIWSTHCHNDLGLAVANSLAAV
                     LNGARQVECTINGLGERAGNASLEEIVMAVRTRRD"
     gene            complement(1102..>1692)
                     /locus_tag="VGA59_09370"
     CDS             complement(1102..>1692)
                     /locus_tag="VGA59_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEX9719927.1"
                     /translation="RIARLTHTESDFGAEYDSLVDMVSFGLAPALIMYEWALSGMGKI
                     GWLAAFIYTAAAGLRLARFNTQVGVADKRYFRGLPSPAAAAVVVGLVWVLHANGVPGK
                     EISIAALIVTVMAGVLMVSNVRFRSFKDVDLRGRVSFMTILAMPLVLVLLFQDPPLVL
                     FLTFLVYALSGPLGFVFRRAHHETKDHPEKHPHGRV"
BASE COUNT          308 a          549 c          535 g          300 t
ORIGIN      
        1 atgtcccggc gcgtgcgcac cgccatgacg atctcctcga gcgaggcgtt gccggcgcgc
       61 tcgcccagcc cgttgatggt gcattccacc tggcgcgcgc cgttgagcac cgccgccagc
      121 gaattggcca ccgccagccc gagatcgttg tggcagtgcg tggaccagat tgccttgtcc
      181 gagttcggca cgcgctcgat gagggtcttc atgcgctcgc cccacacctg cgggatgctg
      241 tagccgacgg tatccggcac gttgatggtg cgggcgccgg cgttgatcgc cgcctcgaac
      301 acgcggcaca ggaaatcgat gtcggaacgc accgcatcct cggccgagaa ctccacgtcg
      361 tcggtgtact ggcgggcgcg tttgaccatc tgcaccgcgc gctccagcac ctgctccggg
      421 gtcatgcgca gcttgtgctg catgtggatc gggctggtgg cgatgaaggt gtgaatgcgt
      481 ccgcgcttcg ccggcttgat ggcctcggcg gcgcggtcga tatccttctc cacggcgcgc
      541 gccagcccgc aaacggtgct ttccgtgacc gcctcggcca cggccttgac cgcctcgaaa
      601 tcgccgggac tggcgaccgg gaaaccggcc tcgatcacgt ccacgcgcat gcgttccagc
      661 accttggcga tgcgcacctt ctcgtcgcgt gtcatggagg cgccgggact ctgctcgccg
      721 tcgcgcagcg tcgtgtcaaa tatgatcaac tgatcgttca tggtcttgtc tctgcgtaaa
      781 tgttgcctgc ttcggtcaaa tggtttccct ccaactcccc ctctcccgcg atagcgggag
      841 agggcaaggg gtgagggcgt gtcctgtcat tcatcgtcgc aaacatgatt cattcaaatc
      901 atgcgggcta taaacaggtt cttggtcgcg tattatattc ctcgccagga agaggttagt
      961 ttgcgcctag cggcgcagca gcagggacag cagagaggca agcgcacggg tcggtgccac
     1021 cgcgcggcgg cgggaccggg tgctgaagga atgggaggat atgccctgca tgactaaagc
     1081 ttaaatcaat ccgggaaaaa attaaacccg gccgtggggg tgtttttcgg gatggtcctt
     1141 ggtctcgtgg tgcgcccgcc ggaacacgaa tcccagggga cccgacagcg catacacgag
     1201 aaaggtcagg aacagcacca gcgggggatc ctgaaacagc agcaccagca ccagcggcat
     1261 cgcgagaatg gtcatgaacg acacccgccc gcgcaggtcc acgtccttga aactgcggaa
     1321 acgcacattg cttaccatca gcacgccggc catcacggtc acgatcagcg ccgcgatgct
     1381 gatctccttg cccggcacgc cattggcgtg cagcacccag accagaccga ccaccacggc
     1441 cgcggccgcc gggctcggca agccccggaa gtagcgcttg tccgccacgc ccacctgggt
     1501 attgaagcgg gccaggcgca ggccggcggc ggcggtataa atgaaggccg ccagccagcc
     1561 gatcttgccc atgcccgaga gcgcccattc atacatgatc agcgcgggcg cgagcccgaa
     1621 cgacaccatg tccaccaggc tgtcgtactc ggcgccgaaa tcgctttcgg tgtgggtcag
     1681 gcgcgcgatg cg
//
LOCUS       DASWIY010000254         5246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1267958, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000254 DASWIY010000000
VERSION     DASWIY010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267958"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VGA59_09375"
     CDS             complement(<1..745)
                     /locus_tag="VGA59_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9719928.1"
                     /translation="MRITADSLPLASAEAAVGHTSAPFFEARELGLAFGGVLAVRDVS
                     FNVDRGEVLALIGPNGAGKTSVLNVVTRVFDATHGTIHFEGQDLTRVPRHEIVRHGIA
                     RTFQNIELFEGATVLENLLLGRHRAGHGSVLAQMLNLPSVQRAEAETRARVEDVVRLL
                     DLASVRDRPVTELPYGLRKVVELGRALASEPRLLLLDEPASGLNPDETRQLAYWIEDV
                     QNELGITIVMVEHDMSLVSAVAGRVLVMDQ"
     gene            complement(742..1776)
                     /locus_tag="VGA59_09380"
     CDS             complement(742..1776)
                     /locus_tag="VGA59_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9719929.1"
                     /translation="MLGAPHAVAVYGALVAALLLLPFLLPTYYVGELSFIFIMCVASL
                     GLMTLTGFTGQISLGQAAFVGIGAYLHAQLLTLGVPLPLAMASAAAGAALAGLLVGIP
                     AIRVSGLHLAMVTLAFAIITEHVLGRWKSVTGGHSGIQVPDPSLFGWSLGSPRAFYFL
                     CLAMLIMVLLLLLNLLRSATGRAFVGIRDSEAASQGLGINVARTKVLAFALSAGTCGL
                     AGALLAHQTQYITPDAFGMALSLQLVLMVFVGGMGSLRGAILGAILIGLLPPLISQLK
                     AFLPRGISGQFGLELFVYGAVLSFFVLLEPRGLNGRWIKLRNWLTRFPMVRAVSSHPT
                     KVYMRSERYR"
     gene            complement(1816..2700)
                     /locus_tag="VGA59_09385"
     CDS             complement(1816..2700)
                     /locus_tag="VGA59_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9719930.1"
                     /translation="MDASLTQFAQLTLQGLAAGAIYGLVALGMVLGYKATEVMNFAHG
                     DIVMLSAFLGWGLIEALHMPFWLALVAVVALVALFCYGLEAKIMRRIVGQPQFAGVML
                     TIGIGYMIRGAVSMSFGPESRSYATPWTGASTHMGNLVLADLSLVIIAAALAVTGLLY
                     VFLRRTSLGIAVHAAAQNQLSAYLCGVRVKRLNSIVWALSGATAALCGLLLAPISLVD
                     IGLWFVMLKALAALVLGGFGSAPGAIVGGLLIGLVEQFSGVYLPDGFKDVVPYLVLIG
                     VLILCPRGLFGEAHGRRV"
     gene            complement(2713..4491)
                     /locus_tag="VGA59_09390"
     CDS             complement(2713..4491)
                     /locus_tag="VGA59_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9719931.1"
                     /translation="MNTYHPPLRDIRFVLHELLQISSGQADSGSGLDLETADQILEAA
                     GRFAAEVIAPLNGVGDQQGCTMPSPGVVQTPAGFKDAYDSFVEAGWTALACRPEHGGQ
                     GLPSVMENAIFEMLCAANMGWAAYPGMSHAAYICLEANGDAAQKALYLPRIASGAWAG
                     TMCLTEPHSGTDLGLLRTRAAPEADGSYRITGTKIFISGGEQDLTENIVHLVLARLPD
                     APPGVKGISLFIVPKFLPAPDGGLGERNELACGSIEHKMGIHGNSTCTLNFEGARGWL
                     LGEANKGLAAMFVMMNHARLIVGVNAIGLMEAAYQKALAYAKERTQGRAPINGNRTAN
                     ADPIIQHADVRRMLLTQKAYVEGSRAFALWAAQLADVQHGDSDPVARGEAAELLALAT
                     PIVKAFSSDLAVECTSLGMQVYGGHGYIVDNHIEQHLRDARIIPLYEGANGIQAMDLL
                     GRKVIADGGERLELYLSKIERFAAYQATHENMREFCEPLLASVSLARDGARAVITASR
                     EDPQALGCAAVPFLRLMGHLSLAWMWARMAAIGQAHSGDSDPIYAAKVATAQFYFQKL
                     LPEIHGLSVSMGHGSAAVMALDVNGF"
     gene            complement(4488..>5246)
                     /locus_tag="VGA59_09395"
     CDS             complement(4488..>5246)
                     /locus_tag="VGA59_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9719932.1"
                     /translation="IAVNTLFNALVNTPELSTVDFSATCLATGAGASVQAAVARRWLE
                     AGGTPITEAYGLSETSPSATFNPPGRNGTIGIPVPSTDVRIVDDEERDVALGAPGELL
                     IRGPQLFAGYWNRPEETRKAFTADGWFKTGDVVTMDEAGFMYMVDRKKDMILVSGFNV
                     YPNEIEAVVAMMPEVLECACVGVSDARCGEVPHLFAVPRQPELTREQIEAHCRRNLAA
                     YKIPRHITLIDALPKSTVGKILRRELRQSEEKAA"
BASE COUNT          987 a         1704 c         1709 g          846 t
ORIGIN      
        1 cctgatccat gaccagcacg cgccctgcca cggcagacac gagcgacatg tcgtgctcca
       61 ccatgacgat ggtgattccc agttcgttct gcacgtcttc gatccaatag gcgagttgcc
      121 gggtctcgtc cgggttcaag ccggaagccg gttcgtcgag cagcagcagg cgcggttcgg
      181 atgcgagggc tcggcccagt tccacgactt tgcgcaggcc atagggcagc tcggtcaccg
      241 gccggtcgcg cacggatgcg agatcgagca ggcgcaccac gtcttcgacg cgtgctcgcg
      301 tctcggcctc ggccctctgg accgacggca gattgagcat ttgcgccagc acgctgccat
      361 ggccggcgcg gtgcctgccc agcagcaggt tctccagcac ggtggcgcct tcgaacagct
      421 cgatgttctg gaacgtgcgg gcgatcccat gccgcacgat ctcgtgccgt ggcacgcggg
      481 tgaggtcctg gccttcgaaa tggatggtcc cgtgggtggc gtcgaaaacc cgcgtgacga
      541 cgttcagcac cgatgtcttg ccggccccgt tcgggccgat cagcgcgagc acctcgccgc
      601 gatcgacatt aaagctgacg tcacgcacgg cgagtacccc gccgaacgcg agtccgagtt
      661 cacgggcctc gaagaagggg gcggatgtgt gccccacggc cgcctctgcg gaagccagcg
      721 ggagtgaatc ggcagtgatt ctcatcggta ccgctccgag cgcatataga ccttggtcgg
      781 gtggctgctg acggcgcgca ccatcgggaa tcgtgtgagc cagttgcgca gcttgatcca
      841 gcggccgttc aggccgcgag gttcgagcag cacgaaaaag ctcaggacgg cgccatagac
      901 aaatagctcc aggccaaact gcccgctgat accacgcggg aggaacgcct tgagctgcga
      961 gatcagaggc ggcagcaacc cgatcaggat ggccccgagg atcgcgccgc gcaggctgcc
     1021 catgccgccg acaaaaacca tcagcacgag ctgcagcgac agcgccatgc cgaacgcgtc
     1081 cggcgtgatg tactgcgtct ggtgtgccag caaggcgcca gccaagccgc aagtacccgc
     1141 cgagagggcg aaggccagca cttttgtgcg agccacgtta atacccagcc cctgggaggc
     1201 ggcctcggag tcacgaatgc cgacaaaagc cctgccggtc gccgagcgca gcaagttgag
     1261 cagcaggaga agcaccatga tcagcatcgc caggcaaagg aagtaaaagg cgcgtgggct
     1321 gcccaggctc catccgaaca gcgacgggtc gggcacctgt atcccgctgt gtccgccggt
     1381 cacgcttttc cagcggccga gcacatgctc ggtgatgatt gcgaaggcga gggtcaccat
     1441 ggccaggtgc aagcccgaaa cccggatcgc cggaatgccc accagcaggc ccgccagcgc
     1501 cgccccggct gccgcgctgg ccatggccag cggcaacggg acacccaagg tgagcaactg
     1561 ggcgtgcagg tacgcgccga tgccgacgaa ggccgcctgg ccgagcgaga tctgaccggt
     1621 gaaacccgtc agcgtcatca accccaagct cgccacgcac atgatgaaga tgaatgacag
     1681 ctcgccaacg tagtaggtcg gaagcaggaa tggcagcaac agcagcgcag caaccaacgc
     1741 gccgtaaaca gccacggcgt gcggcgcgcc gagcaagctg cgcgattgcc ggtaatcagt
     1801 ttgatagctc atgcttcaca ccctccggcc atgcgcttcg ccgaacaggc cgcgcggaca
     1861 caggatcagg actccgatca aaacgagata gggcaccacg tccttgaagc cgtccggaag
     1921 gtaaacgccc gagaactgct cgaccagtcc gatgagcagg ccgccgacga tggcgccggg
     1981 cgcactgccg aaaccgccga gaaccagggc cgccagcgcc ttgagcatca cgaaccacag
     2041 tccgatgtcg acgagcgaga tcggcgccag cagcaggccg cacagcgccg cagttgcgcc
     2101 ggacagggcc cagacgatgg agttcagacg cttgacgcgg acgccgcaga ggtacgctga
     2161 gagttggttc tgggccgccg catgcacggc gatgcccaga gaggtgcggc gcaggaagac
     2221 gtaaagcagc cccgtcacgg caagtgccgc cgcgataatc acgaggctca ggtcagcgag
     2281 gacgagattg cccatgtgcg tggatgctcc cgtccacgga gtcgcgtagc tgcgcgactc
     2341 gggcccgaag gacatgctga cggcacctcg gatcatgtag ccgatgccga tggtcagcat
     2401 cacgccggcg aactggggct ggccaacgat tctccgcatg atcttagcct ccaggccgta
     2461 gcaaaacagc gcaaccaatg cgacgacggc aacgagcgcc aaccagaatg gcatgtgcag
     2521 cgcctcgatc aggccccagc ccaggaaggc cgagagcatg acgatgtcgc cgtgggcgaa
     2581 gttcatgact tccgtggcct tgtagccgag caccatcccc agggcgacca gcccgtagat
     2641 agcgcctgcg gccaggccct gcaaagtcag ctgcgcaaat tgtgtgagcg aggcatccac
     2701 tgggagatcc gttcagaagc cgtttacatc aagtgccatc acggccgcgg agccgtgccc
     2761 catggacacc gacagcccgt ggatctccgg caacagcttc tggaagtaga actgcgcggt
     2821 ggccacctta gcggcgtaga tgggatcgga gtcccccgag tgcgcttggc cgatcgccgc
     2881 cattcgcgcc cacatccacg ccaaggacaa atgccccatg agacgcagga acggcacggc
     2941 cgcgcaaccc agcgcctggg gatcctcgcg cgatgcggtg atgacagcgc gcgcaccgtc
     3001 gcgggccagt gacaccgatg ccagcagcgg ctcgcaaaat tcgcgcatgt tttcgtgcgt
     3061 ggcctggtaa gccgcgaaac gctctatctt ggacaggtag agctcgagcc gttcaccgcc
     3121 atcggcgatc accttgcgac ccagcagatc catggcctgg atgccgttgg cgccttcgta
     3181 gagcggaatg atgcgggcgt cgcgcaggtg ctgctcgata tggttgtcaa cgatgtagcc
     3241 gtggccgcca tagacctgca tcccgagact cgtgcattcc acggccagat cgctgctgaa
     3301 cgccttcacg atcggagtgg ccagggccag cagttcggcg gcttcgcccc gggcaacggg
     3361 atcggagtcc ccgtgttgca cgtccgccag ctgagcggcc caaagcgcga aggcacgcga
     3421 gccttcgacg tacgctttct gcgtgagcag catgcggcgc acgtccgcat gctggatgat
     3481 cggatcggca ttcgccgtgc ggttgccgtt gatgggcgcg cggccttgtg tccgctcttt
     3541 cgcgtaagcc agggccttct gatacgccgc ctccatcagg ccgatggcgt tgacccccac
     3601 gatcaggcgg gcgtggttca tcatcacgaa catcgcagcc agtcccttgt tggcctcgcc
     3661 gagcagccag ccgcgcgcgc cttcgaagtt cagggtgcac gtggaattgc cgtgaatgcc
     3721 catcttgtgc tcgatcgagc cgcaggcgag ttcgttgcgc tcgcccaggc cgccatcggg
     3781 tgcaggcaga aacttgggaa cgatgaacag cgagatgccc ttgacccccg gcggcgcatc
     3841 cggcaaccgg gcgagcacca ggtgcacgat gttctcggtc agatcctgct cgccgccgga
     3901 gatgaagatc ttggttcccg tgatgcggta gcttccatcc gcttcagggg ctgcgcgcgt
     3961 gcgcaacagg cccaggtcgg tcccgctatg cggctccgtc aagcacatcg tgcccgccca
     4021 tgcgcccgag gcaatgcgcg gcaggtacag cgctttttgt gctgcgtccc cgttggcctc
     4081 caggcagatg tacgcggcat gcgacatgcc ggggtaggct gcccaaccca tgttggcggc
     4141 gcagagcatt tcgaaaatcg cgttttccat cacggagggc aggccctggc cgccgtgctc
     4201 cggccggcac gccaacgcgg tccagccggc ttcgacgaag ctgtcgtacg cgtccttgaa
     4261 acccgccggc gtctgtacca cgcccggcga gggcatggtg catccctgct ggtcgccgac
     4321 gccgttcaaa ggtgcgatga cctccgccgc gaaacgcccg gccgcttcga ggatctggtc
     4381 ggcggtttcc agatcgagcc ctgatccgct gtcggcctgg ccgctggaga tctgcagcag
     4441 ttcatgcagc acgaagcgga tgtcgcgcag gggtgggtga taggtgttca tgccgctttc
     4501 tcctcggatt ggcgcagttc acggcgcagg atttttccca cggtggactt cggcaacgcg
     4561 tcgatcaagg tgatgtgccg cggtatcttg tacgcggcca gattgcgccg gcagtgggct
     4621 tcgatctgtt ctcgcgtcag ttctggttgg cgcgggacgg cgaacaggtg cggcacttcg
     4681 ccgcagcgtg catcgctcac gccgacacag gcgcactcga gcacttccgg catcatcgcc
     4741 accaccgcct cgatctcgtt ggggtagacg ttgaacccgg agaccaggat catgtccttc
     4801 ttgcggtcga ccatgtacat gaagccggct tcgtccatgg tgacgacatc gccggtcttg
     4861 aaccagccgt cggccgtgaa cgccttccgt gtctcttccg ggcgattcca gtagccggca
     4921 aagagctggg ggccgcggat caggagttcg cccggcgccc cgagggcaac gtcccgctcc
     4981 tcatcgtcga cgatgcgcac gtccgtggac gggacgggaa ttccgatcgt gccgttgcgc
     5041 cccggcgggt tgaaagtggc actgggggat gtctccgaca ggccgtaggc ctcggtgatc
     5101 ggagtgccgc cggcttccag ccagcgccgg gcgaccgcgg cctggaccga ggcaccggcg
     5161 ccggtggcca ggcaagtggc ggaaaagtcg acggtcgaga gttccggcgt attgaccaac
     5221 gcgttgaaaa gcgtgttgac cgcgat
//
LOCUS       DASWIY010000255         5437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_902267, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000255 DASWIY010000000
VERSION     DASWIY010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902267"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            773..1012
                     /locus_tag="VGA59_09400"
     CDS             773..1012
                     /locus_tag="VGA59_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719933.1"
                     /translation="MLVKRGEITERERLAFERAVRSCGREPDEFRIEAFTAGTASTLR
                     SVHVATSGAAAQYEASDGTTWTLKFAAHLARGCFR"
     gene            1123..2310
                     /locus_tag="VGA59_09405"
     CDS             1123..2310
                     /locus_tag="VGA59_09405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9719934.1"
                     /translation="MGLTADFLVVGGGIAGASVGYWLARHGRVVLLERESQPGYHSTG
                     RSAALFMESYGTPQVRALTMASRAFLESPPDGFTEHPVLSPRGAMMVATHGQEEALQA
                     HWHVLHGMSPRAALLDASGACEVTPVLRREKLLGAVLEPDAMDMDVHAIHQGFLRGIR
                     RNGGSVVSNAEVTALARSGGVWQVRAGGERYEAPIVLNAAGAWAETVAAMAGARPIGL
                     QPRRRSAFIFAPPAGMSSAAWPLTAGVDEDWYFKPDAGMLLGSPANADPVEPHDVQPE
                     LLDIATAIERIQHMTTLVIQRPTHTWAGLRSFVADGDLVGGWDPQVPGFFWVAAQGGY
                     GIQTSPAMGEACAALARGLPMPQRIAAFGLTAEMLAPGRLFAAAAAPLAGSAASSRSQ
                     ETP"
     gene            2307..3038
                     /locus_tag="VGA59_09410"
     CDS             2307..3038
                     /locus_tag="VGA59_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HEX9719935.1"
                     /translation="MTVETLAIDPRISSALMDVSTATVTTVLLKKGLRNVWLRNTRPL
                     AAGQPRLVGRAFTLRFVPAREDLATPASWASPISTRAAIEDMPAGCIAVVGALGVTDA
                     GIFGDILCARMQKRGVAGLVTDGVVRDVAGVLGTGLPVWCQGAAAPPSVAGLTFVGWQ
                     EPIDCGGVAVFPNDVIVVDQDGAVLIPAALLDDVVAACVEQERLETWIMSRVDEGAAL
                     PGLYPPNAENLERYRHWAESGANKG"
     gene            complement(3079..4062)
                     /gene="dhaK"
                     /locus_tag="VGA59_09415"
     CDS             complement(3079..4062)
                     /gene="dhaK"
                     /locus_tag="VGA59_09415"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEX9719936.1"
                     /translation="MKKFTNLPSDLVYESMLGFGAAHAQLVTVHLDPLFVTRKILNPS
                     KVALISGGGSGHEPLHTGFVGVGMLDAACPGQVFTSPTPNQMLAAAKAVGSQAGVLFI
                     VKNYAGDVMNFQMAMDMMDVECASVLVNDDVAIDVAGHPAGRRGVAGTLMVEKIVGAA
                     AERGADLAACKRLGDQVNARTASMGVAFSSCTVPEAGVATFEIGPEEMEIGVGIHGEP
                     GRRRAPFQPATAIVQELMGAILKDLAPKQGQEVLLHVNGLGGTPLMELYLLYQLASEH
                     LCTAGLVPVRSLVGNYTTSLEMAGASLTVTLLTEEIKQLWDAPVATPALHW"
     gene            complement(4134..5162)
                     /locus_tag="VGA59_09420"
     CDS             complement(4134..5162)
                     /locus_tag="VGA59_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9719937.1"
                     /translation="MAATITQIAEAAGVSTATVDRVLNNRPGVNPATVERVRTVMQAL
                     GAASPSRGRPRSTPNYRFAFVLPASRRGFFDMVDRVIAQAAGDFRHQHITEITHRLPN
                     QDGADFAQELAKLTELDGIALLAPDVPQVKLAINELARSGVHVVTLFSDVPGSLRETF
                     VGADNRAAGRTAGVLLGRCLPRSGAGRCAMLSPATRFAAEIDRRIGFAQVMEERYPKA
                     RLLQLHDLPESEDQAYTFARRFLDPAQTEGMLHALYNVGPGSHGVSRALQEFGYDSSL
                     GFVVHDLLEMHRSLLVAHVVSYVLQQDVYYAIMTAAKVLRLLCEEVRGALAVSNPRIE
                     IITAENLA"
BASE COUNT          888 a         1796 c         1840 g          913 t
ORIGIN      
        1 tgcatggggc ttcagcacgg ctcggcagac gggcttccga caatctgcaa tgttccgagg
       61 gatccttcag ccccgaatcg caagttccgg attcacggga atcccggggc atagagggaa
      121 gctacggctc agcgtcgcgc cactcacatc tgtgactgtc acctcagtca gcggctgccc
      181 aggtccacag gcgaaccttc cgggggtaaa cgttgaaggc accctaaagg cgtaacggtg
      241 atcgggaggc atcttgggtc cgaagttcat tgaattcagc acctgtggcg tctcgtaggg
      301 cagacgcagt tcaactcttg ctcccggggc ggtaccaatc agcatccgta ggggcggagg
      361 acgaacgaca acaataccgc ccaccgggtt ggagggatag ttaggcacgt agctaatgat
      421 gaactcaatt ggcggccgtg cggaagcctc gccgccgacc tcgcctgcga aggctgcgca
      481 tccacagatt gagcacagaa cgagagccac cgctggcaac tgccagctgc cgcgtgttgc
      541 ccagacttga tgatcgacgt cagacattgc gaacgtatgt agtgagctgt tgatccggac
      601 aaatctcgaa gacttgtgca acctgggcgt cagaagctag cgtgatccgc acttcagaga
      661 tgcactttcc cgctccgcac gcatagtctt caatcgtaat cctagcgtat tcccggacat
      721 cgaccggatc gcatccttcg ttgaatcggc agatgctatt gcggaggccc cgatgttagt
      781 caagcgcggc gagattactg aaagagagcg cctggcattc gagcgagcgg ttcggtcctg
      841 cggacgcgag cccgacgaat tccggatcga ggcattcacc gcaggtacag catcgacgct
      901 gcgcagcgtc catgtcgcca cgagcggcgc agccgcgcaa tacgaggcga gcgacggcac
      961 gacctggaca cttaagttcg cggcacacct ggcccgcggc tgctttcgct gagtctcggc
     1021 tgcggccgaa tccgctggat gcggatgagc ccgtagcagc gggcccatgc gcgtcgggca
     1081 tccgacaagg atatcggcgc tcccgccccc tagaatggcc gcatgggcct gacagcggac
     1141 ttcctggtgg tgggcggcgg catcgcggga gcctcggtcg gctactggct ggcgcggcac
     1201 ggccgcgtcg tgctgctgga gcgggaatcg cagccgggct accactccac cggccgctcc
     1261 gccgcgctct tcatggaaag ctacggcacg ccccaggtgc gggcgctgac gatggccagc
     1321 cgcgcctttc tcgagtcgcc acccgacggc ttcaccgagc atcccgtgct gagcccgcgc
     1381 ggggcgatga tggtggccac ccatgggcag gaggaggcgc tgcaggccca ctggcatgtc
     1441 ttgcacggga tgtcgccgcg ggcggcgctg ctcgacgcat cgggggcctg tgaggtgacc
     1501 cccgtattgc ggcgcgagaa gctcctgggc gcggtgctgg aaccggacgc gatggatatg
     1561 gacgtgcatg ccatccacca gggcttcctg cgcggcatca ggcgcaacgg cggcagcgtc
     1621 gtgagcaatg ccgaggtcac cgccctggcg cgcagcggcg gcgtctggca ggtgcgggcc
     1681 ggcggcgagc gctatgaggc gccgatcgtc ctgaacgccg cgggcgcatg ggccgagacc
     1741 gtcgcagcca tggcgggcgc gcgccccatc ggcctgcagc cacgccggcg ctccgccttc
     1801 atcttcgcgc cgcctgccgg catgtccagc gccgcctggc cgttgacggc cggggtggac
     1861 gaggactggt atttcaagcc cgatgccggc atgctattgg gctcgcccgc caatgccgat
     1921 ccggtcgagc cgcacgacgt gcagccggaa ctgctcgaca tcgccaccgc catcgagcga
     1981 atccagcaca tgaccacgct cgtgatccag cggcccaccc atacctgggc cgggttgcgg
     2041 tccttcgttg ccgacggcga cctggtcggc ggctgggatc cgcaggtgcc cggcttcttc
     2101 tgggtggccg cccagggcgg ctacggcatc cagacctcgc cggcgatggg agaggcctgt
     2161 gccgcgctgg cacggggcct gccgatgccg cagcgcatcg ccgccttcgg ccttacggcg
     2221 gagatgctgg cgccggggcg gctgttcgct gccgctgccg cgccgctcgc cggttccgct
     2281 gcttccagtc gctcccagga gaccccatga ccgtcgaaac ccttgccatc gatcccagga
     2341 tcagcagtgc cctgatggac gtgtccaccg ccactgtcac caccgtcctg ctgaagaagg
     2401 gcctgaggaa cgtctggctg cgcaacacgc ggccgctcgc ggcggggcag ccgcgcctgg
     2461 tcgggcgtgc cttcacgctg cgcttcgtgc cggcgcgcga ggacctggcc acgccggcgt
     2521 cctgggcctc gccgatttcc acccgcgccg cgatcgaaga catgcccgcc ggctgcatcg
     2581 cggtcgtggg cgcgctcggc gtgacggatg ccggcatctt cggcgacatc ctttgcgcgc
     2641 gcatgcaaaa gcgcggcgtt gcgggcctgg tgaccgacgg cgtggtgcgc gacgtggccg
     2701 gcgtgctcgg cacggggctg ccggtgtggt gccagggcgc ggcggcgcca ccgtcggtcg
     2761 cgggcctgac cttcgtcggc tggcaggagc cgatcgactg cggcggcgtg gcggtgttcc
     2821 ccaacgacgt gatcgtggtc gaccaggacg gcgcggtact gattccggcc gcgctgctcg
     2881 atgacgtggt ggccgcttgc gtggaacagg agcggctcga gacctggatc atgagccggg
     2941 tcgacgaagg cgcggcgctg cccggcctgt atccgcccaa tgccgaaaac ctggaacgct
     3001 atcgccactg ggccgagagc ggcgcgaaca agggctgaag cggcgacgcc gcctgcaggc
     3061 gcgggccgca agcgtgcgct accagtgcaa ggccggcgtc gcgacgggtg catcccaaag
     3121 ctgcttgatc tcttcggtga gcagcgtgac ggtcaacgag gcgcccgcca tctccaggga
     3181 cgtggtgtag ttgcccacca gcgatcgcac cggtaccagc ccggcagtgc acaggtgctc
     3241 gctggccagc tggtacagca agtacagctc catcaggggc gtgccgccca ggccattgac
     3301 gtgcagcagc acctcctggc cctgcttcgg ggccaggtcc ttcaggatgg cgcccatcag
     3361 ttcctgcacg atggcagtgg ccggctggaa cggcgcgcgc cggcggcccg gttcgccgtg
     3421 gatgccgacg ccgatttcca tttcctcggg cccgatctcg aaagtcgcca cgcccgcctc
     3481 cggcacggta caactgctga aggccacgcc catcgaggcg gtgcgggcat tgacctgatc
     3541 gcccagccgc ttgcatgcgg ccaggtcggc accgcgctcg gcggcggcac ccacgatctt
     3601 ctcgaccatc aatgtcccgg ccacgccgcg ccggcccgcg gggtgtcccg cgacatcgat
     3661 cgccacgtcg tcattgacca gcaccgaggc acattcgacg tccatcatgt ccatcgccat
     3721 ctggaaattc atgacgtcac cggcgtagtt cttgacgatg aagagcacgc cggcctggct
     3781 gccgacggcc ttggccgcgg ccagcatctg gttgggcgtg ggtgaggtga acacctggcc
     3841 cgggcaggcc gcatcgagca tgcccacgcc gacaaagccg gtgtgcagtg gctcgtggcc
     3901 cgatccgcca cccgagatca gcgcgacctt ggacgggttg aggatcttgc gcgtgacaaa
     3961 aagcggatcc aggtgcacgg tcaccagctg ggcatgggcg gccccgaacc ccagcatgct
     4021 ttcatacacg aggtcgctcg ggaggttggt gaatttcttc atcgccgctc gcgtggtagt
     4081 tgggccgatt atggccaccc cggcactgtc ctgtcacgcc agggctttgc cggtcatgcc
     4141 aggttctccg cagtgatgat ctcgatgcgg gggttgctca cggccagggc gccgcgcacc
     4201 tcttcgcaca gcagccgcag caccttggcg gcggtcatga tggcgtagta cacgtcctgc
     4261 tgcaggacgt acgagaccac gtgggccacc agcagcgagc ggtgcatctc cagcaggtcg
     4321 tgcaccacga atcccagcga actgtcgtag ccgaattcct gtagggcgcg gctgacgccg
     4381 tgcgagcccg ggcccacgtt gtacagcgca tgcagcatgc cttccgtctg ggccgggtcg
     4441 aggaaacgtc tggcaaacgt gtaagcctgg tcttccgact cgggcaggtc gtgcagctgc
     4501 aacagccggg ccttggggta tcgctcttcc atgacttgcg cgaagccgat gcggcggtcg
     4561 atctcggcgg caaagcgcgt ggcgggcgac agcatggcac aacggccggc gccgctgcgc
     4621 ggcaggcagc ggcccagcag gacgcccgcc gtgcggccgg ccgcacggtt gtccgcgccg
     4681 acgaaggtct cgcgcaggga accgggcacg tcggaaaaca gcgtcaccac atgcacgcca
     4741 cttcgcgcca gctcgttgat ggccagtttg acctgcggca cgtcgggtgc gagcagggcg
     4801 ataccgtcga gctcggtgag cttcgccagc tcctgggcga agtccgcgcc gtcctggttg
     4861 ggcaggcgat gcgtgatctc ggtgatgtgc tgatgccgga agtcgccggc ggcctgcgcg
     4921 atgacgcgat ccaccatgtc gaagaagccg cgtcgcgagg ccggcagcac gaacgcgaag
     4981 cggtagttcg gcgtggaacg cggccggccc cggctggggc tggctgcccc gagcgcctgc
     5041 atcacggtgc gcacccgctc gaccgtggcc ggattgacgc ctgggcgatt gttcagcacg
     5101 cgatcgaccg tggccgtgct gaccccggcg gcttcggcga tctgggtgat ggtggcggcc
     5161 atgatttctt gcgacgtggc aaataaatca atctatcatc cgcgccgcag cccgaattta
     5221 cctcaatttg ggccgctatg gtagcgttgt ttcagcccaa gacgtcgtta tcccaggact
     5281 gatctcgtca tcccggaccc cggatcaagt ccggggcagg cccgatccgg gatttatgcc
     5341 cccctcgaaa catggattgc ggatcaggtc cgcaatgaca gccattttca agccggcttt
     5401 caatgaggta ttagggtttg tcttgattga tgtatta
//
LOCUS       DASWIY010000256         4147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3137492, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000256 DASWIY010000000
VERSION     DASWIY010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3137492"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /gene="secY"
                     /locus_tag="VGA59_09425"
     CDS             <1..815
                     /gene="secY"
                     /locus_tag="VGA59_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX9719938.1"
                     /translation="GEQITERGLGNGISILIFAGIAAGLPSAIGGLLELIRTGAMSIL
                     VAVLILAVVALVTYFVVFVERGQRKILVNYARRQVGNKVYGGQSSHLPLKLNMAGVIP
                     PIFASSIILLPATVVSWFATGESMRWLKDIAGTLSPGQPIYVMLYASAIIFFCFFYTA
                     LVFNSRETADNLKKSGAFIPGIRPGEQTARYIDKILVRLTLAGAVYITFVCLLPEFLI
                     LKYNVPFYFGGTSLLIIVVVTMDFMAQVQNYLMSQQYESLLKKANFKTSLGG"
     gene            822..1040
                     /gene="infA"
                     /locus_tag="VGA59_09430"
     CDS             822..1040
                     /gene="infA"
                     /locus_tag="VGA59_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX9719939.1"
                     /translation="MAKDDVIQMQGEVVENLPNATFRVKLENGHVVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLSRARIVFRAK"
     gene            1061..1174
                     /gene="rpmJ"
                     /locus_tag="VGA59_09435"
     CDS             1061..1174
                     /gene="rpmJ"
                     /locus_tag="VGA59_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX9719940.1"
                     /translation="MRVSASVKKICRNCKIIRRKGIVRVICTDPRHKQRQG"
     gene            1247..1612
                     /gene="rpsM"
                     /locus_tag="VGA59_09440"
     CDS             1247..1612
                     /gene="rpsM"
                     /locus_tag="VGA59_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX9719941.1"
                     /translation="MARIAGINIPPHQHAEIGLTAIFGIGRSRARKICEATGIPYAKK
                     VKDLTDGDMEKIRDQIARFTIEGDLRRETTMNIKRLMDIGCYRGFRHRRGLPMRGQRT
                     RTNARTRKGPRKAAQALKK"
     gene            1631..2035
                     /gene="rpsK"
                     /locus_tag="VGA59_09445"
     CDS             1631..2035
                     /gene="rpsK"
                     /locus_tag="VGA59_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX9719942.1"
                     /translation="MAKAPANTAAQRVRKKVRKNVSDGVCHVHASFNNTIITITDRQG
                     NALSWASSGGQGFKGSRKSTPFAAQVASEVAGRAAIEQGIKNLDVEIKGPGPGRESSV
                     RALAALGIRINSIADVTPVPHNGCRPQKRRRI"
     gene            2162..2785
                     /gene="rpsD"
                     /locus_tag="VGA59_09450"
     CDS             2162..2785
                     /gene="rpsD"
                     /locus_tag="VGA59_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX9719943.1"
                     /translation="MARYLGPKAKLSRREGTDLFLKSARRSLADKAKFDSKPGQHGRT
                     SGQRTSDFGLQLREKQKVKRMYGILERQFRRYFEDAAQRKGNSGANLLFILESRLDNV
                     VYRMGFGSTRAEARQLVSHKAVTVNGRAVNIPSYMVKAGDVIAVREKSKKQNRVVEAL
                     QLAQQVGLPAWVEVNADKAEGVFKKVPDRDEFAADVKEALIVELYSR"
     gene            2918..3907
                     /gene="rpoA"
                     /locus_tag="VGA59_09455"
     CDS             2918..3907
                     /gene="rpoA"
                     /locus_tag="VGA59_09455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX9719944.1"
                     /translation="MQTNLLKPKAINVEQLGINRAKVTLEPFERGYGHTLGNALRRVL
                     LSSMVGYAATEVTIAGVLHEYSSIDGVQEDVVGILLNLKGVVFKLHNRDEVTLSLRKD
                     GEGVVTAADIQTPHDVEIINPDHVIAHLSQGGKIDMQIKVEKGRGYVPGTMRRYADEP
                     TKSIGRIVLDASFSPVKRVSYTVESARVEQRTDLDKLVVEIETNGAITAEDAVRASAK
                     ILVEQLAVFAQLEGSELAAFDQPVQRSSQQFDPILLRPVDELELTVRSANCLKAENIY
                     YIGDLIQRTENELLKTPNLGRKSLNEIKEVLASRGLTLGMKLESWPPAGLDKR"
     gene            4011..>4147
                     /locus_tag="VGA59_09460"
     CDS             4011..>4147
                     /locus_tag="VGA59_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HEX9719945.1"
                     /translation="MRHGHGLRKLNRTSEHRLAMLRNMMNSLLQHEVIKTTVPKAKEL
                     RR"
BASE COUNT          865 a         1317 c         1250 g          715 t
ORIGIN      
        1 tgggcgagca gatcaccgaa cgggggctgg gcaacggtat ctcgatcctg atcttcgccg
       61 gcatcgcggc gggtctgccc agcgccatcg gcggcctgct ggagctgatt cgcaccggcg
      121 ccatgagcat cctggtcgcc gtgctcatcc tggcggtggt ggccctggtg acctacttcg
      181 tcgtgttcgt ggaacgcggc cagcgcaaga tcctggtcaa ctacgcgcgc cggcaggtcg
      241 gcaacaaggt ctatggcggc cagtcgtcgc acctgcccct gaagctgaac atggccggcg
      301 tgattccgcc gatcttcgcc tcgtcgatca tcctgctgcc ggccaccgtg gtgagctggt
      361 tcgccaccgg cgaatcgatg cgctggctca aggacatcgc ggggaccctg tcgcccggcc
      421 agccgatcta cgtgatgctg tacgccagcg cgatcatctt cttctgtttc ttctacacgg
      481 ccctggtgtt caacagccgc gagacagccg acaacctgaa gaagagcggc gccttcatcc
      541 ccgggatacg ccccggcgag cagaccgccc gctatatcga caagatcctg gtgcgcctga
      601 cgctcgccgg cgcggtgtac atcaccttcg tgtgcctgct gcccgaattc ctcatcctga
      661 agtacaacgt gccgttctat ttcggcggaa cctcgctgct gatcatcgtg gtcgtcacga
      721 tggacttcat ggcccaggtg cagaactatt tgatgtcgca gcaatatgaa tcgctgctga
      781 agaaagccaa tttcaagacc tcactcggcg gctagatata gatggctaaa gacgatgtca
      841 tccagatgca gggtgaggtt gtggagaacc tgccgaacgc gacgtttcgc gtgaagctgg
      901 aaaacgggca tgtggtgctc ggacatattt cgggaaagat gcgcatgcac tacatccgca
      961 tcctcccagg tgacaaggtc accgtcgagt tgacgcccta cgacttgtcg cgggcacgga
     1021 ttgtattcag ggccaaataa ctggcttagg agaaagacaa atgagagttt cggcttcggt
     1081 caagaaaatt tgccgcaact gcaagatcat ccgccgcaag ggcattgttc gcgtgatctg
     1141 taccgatccg cgccacaagc agcgccaggg ctgaaatgag taacctgggg tcggaccaaa
     1201 agcgcgcagc gcgtttgctc ggacctcact gatttagagg acgaacatgg cacgtatcgc
     1261 tggcatcaac attccgccgc atcagcacgc cgaaatcggc ctgacggcca ttttcggcat
     1321 tggccgctcc cgcgcccgca agatctgcga ggccaccggc attccatacg ccaagaaggt
     1381 caaggacctc accgacggcg acatggagaa aatccgcgac cagatcgccc ggttcaccat
     1441 cgaaggcgac ctgcgccgcg aaacgaccat gaacatcaag cgtttgatgg acatcggttg
     1501 ctaccggggc ttccgtcatc gccgcggcct gccgatgcgc ggccagcgta cgcgcaccaa
     1561 tgcccgcacc cgcaagggcc cgcgcaaggc tgcccaggca ctcaagaaat aatcgggaaa
     1621 gtattaaatc atggcaaaag ctcccgccaa taccgccgcg cagcgtgtgc gcaagaaggt
     1681 ccgcaagaac gtgtccgacg gcgtgtgcca cgtgcacgcg tccttcaaca acacgatcat
     1741 cacgatcacc gaccgccagg gcaacgccct gtcgtgggcg tcttcgggcg gccagggttt
     1801 caagggttcg cgcaagtcga ctcccttcgc cgcccaggtc gcctccgagg tcgccggccg
     1861 cgcggcgatc gagcaaggca tcaagaacct cgacgtcgag atcaagggcc cgggcccggg
     1921 tcgcgagtcg tccgtccggg cgctggccgc gctgggtatc cgcatcaact cgatcgccga
     1981 tgtgacgccg gttccccata acggctgccg tccgcaaaag cgccgcagga tttaaacaag
     2041 gtgagaacac ggcatccctg cgcgggacgc cttgaacttg agtttttaag cccaccgccg
     2101 gcgacccagg tcaccggctc ccgcggcaac aggctgcggc agatgattca aaggaagcaa
     2161 agtggcacgt tatctaggcc ccaaggccaa actctcacgc cgtgaaggca cggacctgtt
     2221 cctcaagagc gcccgccgct cgctggcgga caaggccaag ttcgactcca agcccggcca
     2281 gcacgggcgc acttcgggcc agcgcacgtc ggacttcggc ctgcagctgc gcgagaagca
     2341 gaaggttaag cgcatgtacg gcatcctcga gcgccagttc cgccgctatt tcgaggatgc
     2401 ggcccaacgc aagggcaact cgggcgccaa cctgctgttc atcctggagt ctcgcctgga
     2461 caacgtcgtc tatcgcatgg gcttcggctc gacccgcgcc gaggcgcgcc agctggtgtc
     2521 gcacaaggct gtcaccgtga acggccgggc ggtgaacatt ccgtcgtaca tggtcaaggc
     2581 cggcgacgtc attgccgtgc gcgaaaagtc caagaaacaa aaccgcgtcg tcgaggccct
     2641 gcagctggcc cagcaggtcg gcctgccggc ctgggtcgaa gtgaatgccg acaaggccga
     2701 aggcgttttc aagaaggtgc cggaccgcga cgaattcgca gccgacgtga aggaagcgct
     2761 gatcgtggaa ctgtattcga ggtaaggagg ataacgagcc tcctgtcgga gtctcgtcct
     2821 tcgagatttt ttgttcccgg ccgcgaggca tcgcggtttg ggtgcttcac cagccttacc
     2881 ggtgtaacga gccgggggta ttgagaggaa gtcctgaatg caaacgaacc tgctgaaacc
     2941 caaagccatc aatgtggaac aacttggcat caaccgtgcc aaggtgacgc tggagccgtt
     3001 cgagcgcggc tacggccaca cgctgggcaa cgccctgcgc cgagttctgc tgtcctcgat
     3061 ggtgggttac gcggcgaccg aagtcaccat cgccggtgtc ctgcacgagt actcgtccat
     3121 cgacggggtc caggaagatg tcgtcggcat cctgctgaac ctcaagggcg tggtcttcaa
     3181 gctccataac cgcgacgaag tcacgctctc cctgcgcaag gacggcgaag gcgtcgtcac
     3241 ggcggccgat atccagacgc cgcatgacgt cgagatcatc aaccccgacc atgtgatcgc
     3301 ccacctgtcg caaggcggca agatcgacat gcagatcaag gtcgagaagg gccgcggcta
     3361 cgttcccggc acgatgcgcc gctacgcgga cgagccgacc aagtcgatcg gccggatcgt
     3421 gctggatgcc tcgttctcgc cggtcaagcg cgtgagctac acggtcgaga gcgcccgcgt
     3481 cgagcagcgc accgacctgg acaagctggt ggtcgagatc gaaaccaacg gcgcgatcac
     3541 cgccgaagac gcagtgcgcg cctcggccaa gatcctggtc gagcagctgg cggtcttcgc
     3601 acagctcgag ggcagcgaac tggccgcctt cgaccagccg gtgcagcgca gctcgcagca
     3661 gttcgacccg atcctgctgc gtcctgtgga cgagctcgag ctcaccgtgc gttcggccaa
     3721 ctgcctgaag gccgaaaaca tctattacat aggcgacctg atccagcgca ccgagaacga
     3781 gctgctcaag acccccaacc tgggccgcaa gtcgctcaac gagatcaagg aagtgctcgc
     3841 ctcgcgcggc ctgaccttgg gtatgaaact ggaaagctgg ccgcccgccg gccttgataa
     3901 gaggtaaacc acaggacaag gcggcgcagc tgccttgtta tcgtctgttt tggtgaacgc
     3961 gtagtacctg atccggctgc gcgaattatt aggtaagaga ggaacacacc atgcgtcacg
     4021 gccacggcct tcgtaaactc aaccgcacca gcgagcaccg gctcgccatg ctgcgcaaca
     4081 tgatgaactc gctgctccag cacgaggtca tcaagaccac cgtccccaag gccaaggagc
     4141 tgcgccg
//
LOCUS       DASWIY010000257        14318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_902583, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000257 DASWIY010000000
VERSION     DASWIY010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 14318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14318
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902583"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VGA59_09465"
     CDS             complement(<1..426)
                     /locus_tag="VGA59_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9719946.1"
                     /translation="MYKRILLAYDGSDAGQKALLDCQDLAQWSQSEVFLIAVMPSAMS
                     FVGLEGGVYDMELEEREKKKYRAVLEDGLRSLAEAGYLARGEVLVGEAVDEITKYARK
                     IEANLIVVGHKHLNSWAARWWRGSISGALIEHAPCSVLVV"
     gene            complement(519..1070)
                     /locus_tag="VGA59_09470"
     CDS             complement(519..1070)
                     /locus_tag="VGA59_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9719947.1"
                     /translation="MRLWLVRHARPLVEAGLCYGALDVAADEGETAAAAQRLAQVLPP
                     SVDVLASPLRRCTQLVHALAAVRPDLAARTEPRLAEMDFGGWEGQLWNRLALSELEAW
                     SADFANYRAGRTGESTAQFIARVGRLLEESRARGHDQAWIAHGGVFKAMLLCQRGISP
                     AAASDWPGQSLAWGQWHLFHIKV"
     gene            complement(1084..1908)
                     /locus_tag="VGA59_09475"
     CDS             complement(1084..1908)
                     /locus_tag="VGA59_09475"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX9719948.1"
                     /translation="MNALARSARGLALALQYFTRVPLPGAVARWTGFDAGLQRASLAH
                     FPAIGLLVAALAIACYWAADRLLPRSGFAPLFAAVFSTAATVLFTGALHEDGLADTAD
                     GLGAARGRDRALEIMKESRIGGFGALALVLALLAKTGLLALIGSVAGWPGAAAALLGG
                     HVSSRALALAIVATLPNVGSAATSKSLPVAARIEPGGLLVAAAWSALGLACAAGLSSW
                     VFCIAGLVAAAIALLWMRRLFARRLKGFTGDGLGATQQTCEIAFYFGAVIALARTV"
     gene            complement(1910..2986)
                     /gene="cobT"
                     /locus_tag="VGA59_09480"
     CDS             complement(1910..2986)
                     /gene="cobT"
                     /locus_tag="VGA59_09480"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX9719949.1"
                     /translation="MTTMHIDLAIRDLADISLAQRLRHKLDQKTKPLGSLGQLEALAL
                     QVGLILGTETPHLVRPQLVVFAADHGLAARGVSAYPGDVTWQMVQNFLGGGAAVSVLA
                     RQHGLSLTVVDCGVARDFAPREGLVIRKIASGTADSLTGAAMSADQCGEAIANGRAIV
                     AQLPGNALLLGEMGIGNTSAASLLLARLTGEPLAECVGAGTGLDEAGLLRKRDVLGRV
                     LAFHAHATAPLEALAAFGGFEIATMVGAVLQAALERRVIVADGFISGAAVLVAQRLAP
                     HVTQRCVFSHRSDEFGHALMLRHLGAGARQPARALLDLGLRLGEGSGAALAWPLLVSA
                     CALLREMASFESAGVSGPQAGARD"
     gene            complement(3037..4521)
                     /locus_tag="VGA59_09485"
     CDS             complement(3037..4521)
                     /locus_tag="VGA59_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX9719950.1"
                     /translation="MKARCVMVLGTTSGAGKSWLATALCRWYARRGLKVAPFKAQNMS
                     NNARVVVGGEIGSAQYFQALAARAEPDVRMNPLLLKPEKDTHSQVVLLGQVDEELTRM
                     DWRGRSASVWPTIARCLDELCAENDVVVIEGAGSPAEINLRDCDIVNMRVAQHAQASC
                     LLVSDIDRGGAFAHLYGTWSLLREAERRLIRGFVLNKFRGDASLLAPAPEVLREQTGI
                     PVVATLPMWWQHGLPEEDGVFDDRSTSGGAVNETIAVVAYPRTSNLDEFQPLKNLPGV
                     RLVWARSPADVAQADCIVVPGSKHTSGDLAWLRAQGLDAAIARHAAAGGAVLGICGGL
                     QMLGEALIDTHGIEENGPGLGLLPLVTVFEPVKTVRRTQARFGAVSGTWAALSGVTMQ
                     GYEIHHGRTFQHPGMQAARVAIPQGLGWQNQEGNVLGVYLHGMFEDAAVLHALFGATA
                     PTLDSVFEGLADFIGRHFRPAVIADLIRDPAGDNNNLDSGSSPE"
     gene            complement(4518..5474)
                     /locus_tag="VGA59_09490"
     CDS             complement(4518..5474)
                     /locus_tag="VGA59_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family cobalamin biosynthesis protein"
                     /protein_id="HEX9719951.1"
                     /translation="MPTWTAVIALLFSFGLDLWWSGRPVSRWHPMVWMRKYMGWMGGR
                     IAPRAPMPSPDWLAFSAGALGWYGGASVAVMLAWLFQWAAISLLDSALAGLALGAMLK
                     TMMSWRLHRLEVIEVETALAQSLDAGRQQLGRLIDCEATDLDEAEVRESAIESLAANL
                     SFWVVAPVFWFLVAGLPGAVFYRFANTAGVMWGYRGFRAGADWEWAGKWAALADDVLS
                     WLPSRITAVLIGVLAGGLPWRELRREAGRTPSSNRGWPTAAMALALGRRLRKPGDRVL
                     NRTAGMPQPGDTARALTLSGRAVAVLLAWACLAYFLAWERPW"
     gene            complement(5489..6322)
                     /locus_tag="VGA59_09495"
     CDS             complement(5489..6322)
                     /locus_tag="VGA59_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9719952.1"
                     /translation="MSSWAAPEPNTAARSGAVFRTSQEGVALLADGLRVTLGGTEVLH
                     GIDLALPAGRWTAIVGPNGAGKTTLLKALAQLLPHQGRVQLLDREASSWSHRERARLL
                     AWLGQNQGGAEDLLAWDVVMLGRLPHQAWLGAPGVKDRAAVEAAMRETQCWDWRDRAL
                     GSLSGGERQRVLLARALAVQAPLLLMDEPLANLDPPHQADWLRIVRRHVERGGTALSV
                     LHEITMALHADEIVLMDAGRVVHQGRCAHGATHSALERVFQRRISVHSLAGQWVALPN
                     E"
     gene            complement(6447..7409)
                     /locus_tag="VGA59_09500"
     CDS             complement(6447..7409)
                     /locus_tag="VGA59_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX9719953.1"
                     /translation="MALLLLGVTGLLCAAGVAVGSLGWEPLWRGGGEASAAILWDIRL
                     PRTLGAWLAGALLGLAGAVAQGLFRNPLADPYLLGSATGAALGVAVLLVLAGAAPAAA
                     SGWMEGLGLTGAAFAGAVVAVLVTLGLAKGVQHTLRLLLAGVVVGMVFGALAALVMFW
                     VPDVMRAMQAFLLGSTGFLSWASVGLLAVLFMVCLLMAVAFSRSLDALSLGETTAASL
                     GIALAPVRMVLIGVMALATGTAVAQVGLIAFIGLVAPHLVRAAIKPTHRWLLPLSALT
                     GGVLLLAADVLARWLLAPQELPVGVLTAVLGGGYLLWLMHRRPL"
     gene            complement(7439..8302)
                     /locus_tag="VGA59_09505"
     CDS             complement(7439..8302)
                     /locus_tag="VGA59_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX9719954.1"
                     /translation="MIRWLLVLACAFALQARALQVTDDRGVVLDLPQPPARIVTLLPS
                     LTEVVCELGACERLVGVDNYSNWPPSVRKLPRLGGLEDANIEAIVALKPDLVLMGYSS
                     RSMGRLESLGIKVMSLEPENLPDLRRVWGQVGEALAANNAQQLWERLQAGVDAAARSL
                     PAPLRGTTVYFEVNSGPYAASEASFIGELLARIGAANVVPGLLGPFPKLNPEFVVRAD
                     PQVIMISDRNAQALKDRPGWDRIRAVRAGRVCMFTPEQGDMLVRPGPRMAAAAGLMVD
                     CLQGRLKGGAP"
     gene            complement(8299..9060)
                     /locus_tag="VGA59_09510"
     CDS             complement(8299..9060)
                     /locus_tag="VGA59_09510"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF014351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX9719955.1"
                     /translation="MAELSIARSELILGGQKSGKTARAESLAGAWLARSPGHRAVYIA
                     TAQAWDEEMRERIERHRRQRADRVPRMITVEEPLELAHAIGSHSRADTLIVVDCLTLW
                     LTALLMPVVPHAGPEPSPHPNPLPEGEGIKPEAAGPQPEGEGIKPEAAGPQSEAEGTK
                     PDAGGSASSSSLSPWERAGVRAAPATTIANALRACAGPIVLISNEIGAGVIPMGRDTR
                     AFVDALGGLNQQAAAACERVTLMSAGLPLTLKGAS"
     gene            complement(9053..9949)
                     /locus_tag="VGA59_09515"
     CDS             complement(9053..9949)
                     /locus_tag="VGA59_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX9719956.1"
                     /translation="MRRFIAHGPQRIVCLTEETTEWLYLLGQEARIVGISGYTVRPRR
                     AREEKPKVSAFLNAKIDKILELRPDCVFGFSDLQADIAAELIRKGVQVTVFNQRSVDE
                     IFSMMYQVAAMVGQAQRGLELMERMRERLSAIEQAARALPRRPKVFFEEWDEPHISAI
                     RWVSELVAIAGGDDCFPELASQPLGKDRIIVDGAEIARRNPDIVIGSWCGKKFRPEKV
                     AARPGWEAVGAVTNKQLFEIKSADILQPGPAALTDGADQLHRIISRWSNTASTLVAQA
                     TALPPEGAARALGRPGDAEAHG"
     gene            complement(9963..11897)
                     /locus_tag="VGA59_09520"
     CDS             complement(9963..11897)
                     /locus_tag="VGA59_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9719957.1"
                     /translation="MNALLVSPRASALCLALAAAFPSSFAQAPALKETVVTATRFAEP
                     AQSLPLGVSVVTADEIQASGAATVNEALVRVLGIVGRQDFYGGGEYNLDLRGFGATAD
                     NNQVVIVDGLRLSEADLGGTRLAGIPIESVERIEVLRGSGAVLYGEGATGGVIIITTK
                     AGTGKDRRNTASAYAGAGSHRLRDLRAAATVAGGGFSLDATGQKRDTDNHRDNFRSET
                     DAASVTGQWSNDWLRLGARAARDSLDTGLPGGLTTAQYEANPRQTTTPNDKAHIRNAR
                     HSVFAEAHLGDWQLGADAGRREKSLTSLNSGFPFDYDTDAANHSLRARHEARLGGARN
                     LLVLGTDYARWTREVLGDFGSRAGQSSRAWYAKDDVTLAGGTRIGAGYRTEKIAKDNS
                     NASSGLSDRQHAWEIGVSQPLAAGTTVYGRAGRSYRLANVDEFSFTSPATTLKPQTSR
                     DLELGARWAWGAGKVEARLYRSLLDNEIGFDPNAAGPFGFPGANINFDPTRRQGLELD
                     TSHALARTLGLRVNAGLRRASFRSGPYAGRDVPLVPRKTLALRADWTPAAGHRVTGGV
                     NWVSSQHPDFNNACTMPAYTTADVRYAYQWQQLELSLGVSNLFDKKFFTQAFGCAAGV
                     TTAIYPEAGRAVKAALRMRF"
     regulatory      complement(11972..12216)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12253..12465
                     /locus_tag="VGA59_09525"
     CDS             12253..12465
                     /locus_tag="VGA59_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF904 domain-containing protein"
                     /protein_id="HEX9719958.1"
                     /translation="MPSAPQIEQIAERVERLLVRYEELQRTNTLLAAQVEVLTQERDS
                     LKSRLSAARARVEALLERLPETKSAA"
     gene            12497..12850
                     /locus_tag="VGA59_09530"
     CDS             12497..12850
                     /locus_tag="VGA59_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX9719959.1"
                     /translation="MKQLEVQIMGQSYLLACPDGGQQRLLDAVEKVDTAMCRIRDAGK
                     VKARDRIAVLAALNLAFDVNERALPFQGTGFAPSELAANGNGNGNGNGHAHDPRLAQL
                     LARLDSALGDDGRLI"
     gene            13320..14120
                     /locus_tag="VGA59_09535"
     CDS             13320..14120
                     /locus_tag="VGA59_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9719960.1"
                     /translation="MLLEPQLILELALLGSATGFLAGLLGIGGGMLMVPFMTIILTAK
                     GYPGEYTVKMAVATSLATICFTSLSSVRAHARRGAVLWPIVRVLAPGILLGSLAGAQL
                     AAALPGKALSTLFAIFVAFSATQMFLDRKPKPSRTMPGALGTFGMGGLIGLLSSLVGA
                     GGAFVSVPFMTWCNVKIHDAVGTSAALGFPIALAGTLGYLWAGRDLPQMPAGSVGYLY
                     LPGLAVISLASISTAPVGARTAHRMDIRPLRKIFALVLYILAAYFLLR"
BASE COUNT         2283 a         5140 c         4782 g         2113 t
ORIGIN      
        1 aacgaccagc acgctgcagg gcgcatgctc gatcagcgcg ccggaaatgg agccgcgcca
       61 ccagcgcgcg gcccagctat tcaggtgctt gtggcccacc acgatcaggt tggcttcgat
      121 cttgcgggcg tacttggtga tctcgtccac cgcctcgccc accaggactt cgccgcgcgc
      181 caaatagcca gcctcggcca ggctgcgcag gccgtcttcg agcacggccc ggtacttctt
      241 cttctcgcgc tcctcgagtt ccatgtcgta gacgccgcct tccaggccga cgaagctcat
      301 ggccgacggc atgactgcga tcaggaacac ttcggattgg ctccactgcg ccaggtcctg
      361 gcagtccagc aaggctttct ggccggcatc ggagccgtcg taggccagca ggattctctt
      421 gtacatatcg ctctcccggt tgcggcgcgg cgaggccacg cgatacaagc atagaccggg
      481 gcaggccctg gcacaaccgg agtcttgcca ggaagcgatc agaccttgat gtggaacagg
      541 tgccactgtc cccacgcgag gctctggccc ggccaatcgg atgccgccgc cgggctgatg
      601 ccgcgctggc acagcagcat ggccttgaac acgccaccgt gggcgatcca cgcctggtcg
      661 tgcccgcggg cgcgcgactc ttccagcagc cggccgaccc gggcgatgaa ttgcgcagtg
      721 gattcgccgg tacgccccgc gcggtaattc gcgaagtcgg ccgaccaggc ctcgagctcg
      781 cttagcgcca gacggttcca gagctgccct tcccagccac caaagtccat ctctgccagc
      841 cgcggctcgg ttcgcgcagc caggtccgga cgcacggcgg ccagcgcatg caccaactgc
      901 gtgcaacgac gcagcgggga agccagcacg tccaccgacg gcggcaggac ctgggccagc
      961 cgctgcgcgg ccgcggccgt ttcgccttcg tcggccgcga cgtcgagggc gccatagcac
     1021 aggccggctt cgacgagggg ccgcgcgtgg cgcaccaacc agagccgcac gatcaggtcc
     1081 tgttcacact gttcttgcca gcgcgatgac ggcgccgaaa tagaaggcga tctcgcacgt
     1141 ctgttgcgtc gcgcccaggc cgtcgccggt gaagcctttc agtcggcgcg cgaacagccg
     1201 gcgcatccac agcaaggcga tcgcggccgc caccaggccc gcgatgcaga acacccatga
     1261 agaaaggccg gcggcgcagg ccaggccgag ggccgaccag gcggccgcga ccagcaggcc
     1321 acccggttcg atacgcgcgg cgaccggcag cgacttggat gtggcggcgc tgccgacgtt
     1381 cggcaacgtg gcgacgatgg cgagcgccag agcacgcgag ctcacgtggc cgcccagcaa
     1441 ggcggcggcc gcgcccggcc agccggccac cgagccgatg agggccagca aaccggtctt
     1501 ggccagcaag gccagcacca gcgccagcgc accgaagcca ccgatgcgcg actccttcat
     1561 gatctccagg gcgcggtcgc gcccgcgcgc ggcgccgagt ccatcggccg tatcggccag
     1621 gccgtcctcg tgcagcgccc cggtgaacag caccgtggcg gccgtggaga aaacggccgc
     1681 gaacagcggc gcgaagcccg agcgcggcag aaggcggtcc gcggcccaat agcaggcaat
     1741 ggccagcgcc gccaccagca ggccgatagc gggaaaatgc gcgaggctgg cgcgctgcaa
     1801 gccggcgtcg aagccggtcc atcgtgcgac cgcgccgggc agcggcacgc gtgtgaagta
     1861 ctgcagagcc agcgcgaggc cgcgcgcgga tcgggccagg gcgttcacgt cagtcccgcg
     1921 cgccggcctg cggccccgac acgcccgccg attcgaagct cgccatctcg cgcagcagcg
     1981 cgcaggccga gaccagcaac ggccacgcga gcgcggcgcc ggaaccctcg cccagccgca
     2041 gcccgaggtc cagcagcgcg cgcgccggct gccgcgctcc ggcgcccagg tgacgcagca
     2101 tcagcgcgtg accgaactca tcggaacggt gagaaaacac gcagcgctgc gtgacatgcg
     2161 gtgcgagccg ctgcgccacc agcacggcgg cgccggagat gaaaccgtcg gccacgatca
     2221 cgcggcgctc gagcgcggcc tgcagcaccg cgcccaccat ggtcgcgatc tcgaaaccgc
     2281 cgaaggcggc cagcgcttcc agcggcgcgg tggcgtgcgc gtgaaaggcc agcacccgcc
     2341 ccaggacgtc gcgcttgcgc agcaggccgg cttcatccag cccggtgccc gcacccacgc
     2401 attcggccag cggctcgccc gtcaatcgcg ccagcagcag cgatgccgcc gaggtattgc
     2461 cgatacccat ttcgcccagc agcaacgcgt tgccgggaag ctgcgcgacg atggcccggc
     2521 cgttcgcgat ggcctcgccg cattgatcgg ccgacatggc cgctccagtg agggaatcgg
     2581 ccgtgccgga tgcgatcttg cggatgacga gcccttcgcg cggggcgaag tcgcgcgcca
     2641 cgccgcagtc caccaccgtg agggacagcc cgtgctggcg cgcgagcacg ctcacggcgg
     2701 cccctccgcc caggaagttc tgcaccatct gccaggtcac gtcgcccggg taagccgaga
     2761 caccgcgggc cgccaggccg tgatccgcgg caaagaccac cagctgcggc cgcaccaagt
     2821 gcggcgtttc ggtgcccagg atcagaccga cctgcaaggc cagcgcctcc agctgcccca
     2881 gcgagccgag cggcttggtc ttttggtcca gcttgtgccg caggcgctgg gcaagagaga
     2941 tgtcggcgag atcgcggata gcaagatcga tatgcatggt cgtcattccg ggcctgaacc
     3001 cttggcttgt cattccgggc ttgacccctt ggcttgtcat tccgggcttg acccggaatc
     3061 caggttgttg ttgtctcccg ccgggtcccg gatcaggtcc gcgatgacgg cagggcggaa
     3121 gtggcggccg atgaaatcgg cgaggccctc gaacacgctg tccagcgtcg gggccgtcgc
     3181 gccgaacagc gcatgcagca cggccgcatc ctcgaacatg ccgtgcaggt acacgcccag
     3241 cacgttgccc tcctggttct gccagcccag gccctgcgga atagcgacgc gcgcggcctg
     3301 catgccggga tgctggaagg tgcggccatg gtggatttcg taaccctgca tggtcacgcc
     3361 cgacagcgcg gcccacgttc cgctcacggc gccgaagcgg gcctgcgtgc gcctgacggt
     3421 cttcaccggc tcgaacaccg tcaccagcgg cagcaatccc aggcccggcc cgttctcttc
     3481 gatgccgtgc gtgtcgatca gcgcctcgcc gagcatctgc aggccgccgc agatgcccag
     3541 caccgcaccg cctgccgccg catgccgcgc gatcgcggcg tcgaggcctt gcgctcgcaa
     3601 ccaggcaagg tcgccgctcg tgtgcttgga accgggcacc acgatgcaat cggcctgcgc
     3661 cacgtcggcc ggactgcgtg cccacaccag gcgcacgccg ggcaggttct tcagcggctg
     3721 gaactcgtcg aggttgctgg tgcgcggata ggcgaccacc gcgatcgtct cgttgacggc
     3781 gccgcccgac gtgctgcggt cgtcgaacac accgtcttct tccggcagcc cgtgctgcca
     3841 ccacatgggc agggtcgcga ccaccgggat gccggtctgc tcgcgcagca cctcgggcgc
     3901 cggcgccagc agcgaggcat cgccgcggaa cttgttgagg acaaagccgc ggatcagtct
     3961 tctctcggct tcgcgcagca gcgaccaggt gccgtagaga tgggcgaatg cgccgccccg
     4021 atcgatgtcg ctgaccagca ggcaggaggc ctgggcatgc tgcgccacgc gcatgttgac
     4081 gatgtcgcag tccctcaggt tgatctcggc cggcgagccg gcgccttcga tcaccaccac
     4141 gtcgttttcc gcgcacagct cgtccaggca gcgggcgatc gtcggccaca cgctggcgct
     4201 gcggccgcgc cagtccatgc gcgtgagttc ctcgtccacc tggcccagca gcaccacctg
     4261 gctgtgggtg tctttctcgg gcttgagcaa cagcgggttc atgcggacgt ccggctcggc
     4321 ccgggccgcc agcgcctgga aatattgcgc gcttccgatc tctccaccca cgacaacgcg
     4381 ggcattgttg ctcatgttct gtgccttgaa cggcgcgacc ttgaggcccc ggcgcgcgta
     4441 ccagcggcac agagccgtgg ccagccagct cttgcccgcg ccgctggtcg tgcccagcac
     4501 catgacgcaa cgtgccttca ccagggacgc tcccaggcca ggaagtaggc gaggcaggcc
     4561 caggccagca acaccgccac ggcacggccc gacaacgtga gcgcgcgggc cgtgtcgccc
     4621 ggctggggca tgccggccgt tcgattgagg acgcggtcgc cgggcttgcg caggcgccgg
     4681 ccaagcgcca gtgccatcgc cgccgtcggc caccccctgt tcgaggaagg cgttcgcccg
     4741 gcttcgcgcc gcaactcgcg ccagggcaga ccgccggcga gtaccccgat caggaccgcg
     4801 gtgatgcgcg acggcagcca cgacagcacg tcgtcggcga gggccgccca cttgcccgcc
     4861 cattcccaat cggcaccggc gcgaaaaccc cggtagcccc acatgacacc ggccgtgttg
     4921 gcaaaccggt agaacacggc accgggcagg cccgcgacca ggaaccagaa caccggggcc
     4981 acgacccaga aactgaggtt cgcggcgagg gattcgatgg cgctttcgcg aacctcggcc
     5041 tcgtccagat cggtcgcctc gcagtcgatc aggcggccca gctgctggcg gcccgcgtcc
     5101 agggactgcg cgagcgcggt ctccacctcg atgacctcga ggcggtgcaa gcgccatgac
     5161 atcatggtct tgagcatggc gcccagcgcc aggccggcca gcgcggagtc cagcagcgag
     5221 attgccgccc actgaaacag ccacgcgagc atcacggcca ccgaggcgcc gccataccag
     5281 cccagggccc cggccgagaa tgcgagccag tcgggcgacg gcatcggggc gcgcggggcg
     5341 atgcggccgc ccatccagcc catgtacttg cgcatccaaa ccatgggatg ccagcgcgag
     5401 accggccggc ccgaccacca cagatccagg ccgaaggaga acagcagggc gatgacagcg
     5461 gtccaggtcg gcataggcgt tcgccgggct actcgttggg cagggcgacc cactgcccgg
     5521 ccagggaatg cacactgatg cgccgctgga acacccgctc cagcgccgag tgggtggcgc
     5581 catgcgcgca acggccctgg tgaaccaccc ggccggcgtc catcaggacg atctcgtcgg
     5641 catgcagcgc catcgtgatt tcgtgcagca cgctcagcgc ggtgccgccg cgttccacgt
     5701 ggcgccgcac gatgcgcagc caatcggcct ggtgcggcgg gtcgagattg gccagcggtt
     5761 cgtccatcag cagcaatggc gcctgcacgg ccagcgcccg cgccagcaat acgcgctggc
     5821 gctcgccgcc cgagaggctg cccagcgcgc gatcgcgcca gtcccagcac tgcgtctcgc
     5881 gcatggccgc ttccaccgcg gcgcggtctt tcacgccggg cgcgccgagc cacgcctggt
     5941 gcggcaggcg cccgagcatc accacgtccc acgcgagcaa gtcctccgcc ccgccctggt
     6001 tctgccccag ccacgccagc aagcgcgcgc gctcccggtg gctccaggag gaagcctcgc
     6061 ggtccagcaa ctgcacgcgg ccctggtgcg gcagcaactg ggccagcgcc ttgagcaatg
     6121 tcgtcttgcc cgcgccgttg ggcccgacga tcgcggtcca gcggccggcc ggcagcgcca
     6181 ggtcgattcc gtgcagcacc tcggtgccgc ccagcgtgac gcgcaggcca tcggccagca
     6241 atgcgacgcc ctcctggctg gttcggaaaa ccgccccgct acgcgccgcg gtattcggct
     6301 cgggcgcggc ccaactgctc atgccgcgcc ctccgacagc tcgcagttca ggcaggcgcc
     6361 ggggggtcgg gcgcttgcgc tattcgcgct ggtcgatccc cgcacgcggg gcccctcgcc
     6421 cttcacgctc atagcgccct ccgcgctcat agcggcctcc ggtgcatgag ccacagcagg
     6481 tagccgccgc ccagcaccgc ggtgagcacg cccaccggca gttcctgcgg cgcgagcagc
     6541 cagcgcgcca gcacgtcggc ggcgagcagc agcacaccgc cggtgagggc cgacagcggc
     6601 aacagccagc ggtgcgtcgg cttgatggcc gcgcgaacca ggtgcggcgc gaccaggccg
     6661 atgaacgcga tcagtccgac ctgggcgacg gcggttcccg tcgccagggc catcacgccg
     6721 atcagcacca tgcgcaccgg cgcgagcgcg atccccaggc tggcggcggt ggtctcgcca
     6781 agggacagcg catcgagcga gcggctgaag gccacggcca tgagcaggca gaccatgaaa
     6841 agcactgcga gcagccccac gctggcccag ctgagaaaac ccgtcgatcc cagcaggaag
     6901 gcctgcatcg cgcgcatgac atccggcacc cagaacatca ccagcgccgc gagcgcgccg
     6961 aacaccatgc cgaccacgac gccggccagc agcagccgca gcgtgtgctg cacgcccttg
     7021 gccagtccca gcgtgaccag caccgccacc acggcaccgg cgaaagccgc cccggtcagg
     7081 cccaggccct ccatccagcc cgaagcggcc gccggcgcag cgccggccag caccagcagc
     7141 acggccacgc cgagcgcggc gccggtcgcg ctccccagca gataaggatc ggccagtgga
     7201 ttgcggaaga gcccttgcgc cacggcgccc gccagcccca gcagcgcgcc cgccagccat
     7261 gcgcccaggg tgcgtggcag gcggatgtcc cacaggatgg ccgcggaggc ttcgccgccg
     7321 ccgcgccaca gtggctccca gcccaggctg cccaccgcaa cgccggcggc gcacagcagc
     7381 ccggtcacgc ccagcagcag cagcgccagt ggcacggggg tgcgcgcggc cggcagggtc
     7441 acggcgcccc gcccttcagc cgcccctgca ggcagtccac catcagccct gccgcagcgg
     7501 ccatgcgcgg cccgggacgc accagcatgt cgccctgttc gggcgtgaac atgcacacgc
     7561 ggccggctcg caccgcgcgg atccggtccc agcccgggcg gtccttcagc gcctgcgcgt
     7621 tacggtccga aatcatgatc acttgcggat cggcgcgcac cacgaactcg gggttgagct
     7681 tgggaaaggg cccgagcagc ccgggcacca cgttggcggc gcctatgcgc gcgagcagct
     7741 cgccgataaa ggacgcttcg ctggcggcat aggggccgct gttgacctcg aagtacacgg
     7801 tcgtgccgcg caacggcgcc ggcaggctgc gcgcagccgc gtccacgccc gcctgcaggc
     7861 gttcccagag ctgctgcgcg ttgttcgccg ccagcgcctc gccgacctgc ccccacaccc
     7921 gccgcaggtc cggcaggttc tcgggctcca gcgacatcac cttgatcccc aggctctcga
     7981 gccggcccat cgaacgcgag gagtaaccca tcaggaccag gtccggcttg agcgccacga
     8041 tggcctcgat gttggcatct tccagcccgc ccagtcgggg gagcttgcgc accgacggcg
     8101 gccagttgga gtagttgtcc acgccgacga ggcgctcgca cgctcccagt tcgcacacga
     8161 cttcggtgag cgacggcagc aaggtgacga tgcgcgcggg tggctgcggc aggtcgagca
     8221 ccaccccgcg gtcatccgtc acctgcaatg cccgggcctg caaggcgaac gcgcatgcca
     8281 ggacaagcag ccaccggatc atgaggcacc cttgagcgtg agcggcaggc ccgccgacat
     8341 cagcgtcacc cgttcgcagg ccgccgccgc ctgctggttc aggccgccga gcgcgtcgac
     8401 gaaggcacgc gtgtcgcgac ccatcggtat gacgcccgcg ccgatctcgt tgctgatcag
     8461 aacgatcggc ccggcgcagg cgcgcagcgc gttggcgatg gtggtggcgg gggcagccct
     8521 cacccctgcc ctctcccagg gggagaggga ggaagaagat gcagatcccc ccgcatccgg
     8581 tttggtcccc tctgcctctg attgcggccc cgctgcctcc ggtttgattc cctctccctc
     8641 tggttgcggc cccgctgcct ccggtttgat tccctctccc tctgggagag ggttagggtg
     8701 agggctcggt tccgggcccg catgaggtac gaccggcatc agcaacgccg tcagccacaa
     8761 ggtgaggcag tcgaccacga tcaaggtgtc ggcgcggctg tgactgccga tggcatgcgc
     8821 cagctccagc ggttcctcca ccgtgatcat gcgcggcacg cggtccgcgc gctggcgccg
     8881 atggcgctcg atgcgctcgc gcatctcctc gtcccaggcc tgcgccgtgg cgatgtacac
     8941 cgcgcgatgc ccgggcgaac gcgccagcca ggcgccggcc agcgattcgg cgcgcgcggt
     9001 cttgccgctt ttctggccgc ccaggatcag ttcgctgcgc gcgatgctca gttcagccat
     9061 gggcctccgc gtcgccgggc cgccccaagg cgcgggccgc cccctcgggg ggcagcgcag
     9121 tggcttgggc caccagagtg gaggcggtat tgctccaccg gctgatgatg cgatgcagct
     9181 gatcggcgcc gtccgtcaat gccgcgggcc cgggctgcag aatgtcggcc gacttgatct
     9241 cgaacagctg cttgttcgtt accgccccga ctgcctccca gcccgggcgc gccgccactt
     9301 tctccggcct gaatttcttg ccgcaccagg aaccgatgac gatgtcgggg ttgcgccgcg
     9361 cgatctcggc gccgtcgaca atgatgcggt ccttgcccag gggctgcgaa gccagttcgg
     9421 gaaagcagtc gtcgccgccc gcgattgcga cgagctccga cacccagcgg atcgcgctga
     9481 tgtgcggctc gtcccattcc tcgaagaaga ccttcggccg gcgcggcagc gcgcgcgccg
     9541 cctgctcgat cgcggacagc cgctcgcgca tgcgctccat cagctcgagg ccgcgctgcg
     9601 cctgccccac catcgcggca acctgataca tcatcgagaa tatttcatcg acgctgcgct
     9661 ggttgaacac cgtgacctgc acgcctttcc tgatgagttc ggccgcgatg tcggcctgca
     9721 gatcggaaaa gccgaacacg cagtcgggcc gcagctcgag gatcttgtcg atcttcgcgt
     9781 tcaggaaagc gctgaccttg ggtttttctt cccgggcccg gcgcggccgc accgtatagc
     9841 ccgagatgcc cacgatgcgc gcttcctgcc ccagcaggta cagccattcc gtcgtctcct
     9901 cggtcaggca gacgatgcgc tgcgggccgt gggcgatgaa gcgacgcatg acagccacgt
     9961 cctcagaacc gcatccgcag cgcggccttg acggcgcgtc cggcctcagg atagatggcc
    10021 gttgtcacgc ccgccgcgca tccgaatgcc tgcgtgaaga acttcttgtc gaagaggttg
    10081 gaaacgccca gggacaattc aagctgctgc cattgatagg cgtagcggac atcggcggtc
    10141 gtataggccg gcatggtgca ggcgttgttg aagtccggat gctgcgagga tacccagttc
    10201 acgccgcccg tgacacggtg cccggcggcc ggcgtccaat cggcccgcaa cgcgagcgtc
    10261 ttgcgcggca ccagcggcac gtcccgtccc gcataggggc cggagcgaaa gctcgcccgg
    10321 cgcagcccgg cattgacgcg caagcccagc gtgcgggcga gcgcatgcga tgtgtccagc
    10381 tccagtccct gcctgcgggt tgggtcgaaa ttgatgttgg cgcccggaaa accgaaaggc
    10441 ccggcggcgt tgggatcgaa gccgatctcg ttgtccagca ggctgcggta aaggcgcgct
    10501 tccaccttgc ccgcgcccca ggcccagcgc gcgcccagtt ccaggtcacg cgaggtctgc
    10561 ggtttcaggg tcgttgcggg cgacgtgaag ctgaattcgt ccacgttggc caggcggtag
    10621 ctgcgtccgg cgcggccgta gaccgtcgta ccggcggcca gcggctggct cacgccgatt
    10681 tcccacgcat gctggcggtc cgagaggccg gacgaggcgt tgctgttgtc cttggcgatc
    10741 ttctcggtgc gatagccggc gcctatgcgc gtgccgccgg ccagggtgac gtcatccttc
    10801 gcataccagg cgcgcgagga ctgtccggcc ctggatccga agtccccgag cacctcgcgg
    10861 gtccagcgcg cgtagtcggt acccagcacc agcaggttcc tcgcgccgcc caggcgcgcc
    10921 tcgtggcgcg cgcgcagcga atggttcgcc gcgtccgtgt cgtagtcgaa ggggaaaccg
    10981 ctgttgaggc tggtcaggct cttctcgcgc cggccggcgt cggcgccgag ttgccaatcg
    11041 cccaggtgcg cctccgcgaa cacgctgtgg cgcgcgttgc ggatgtgggc cttgtcgttg
    11101 ggcgtggtgg tctggcgtgg attcgcctcg tactgtgctg tcgtgagccc gccgggcaag
    11161 cccgtgtcca gcgaatcgcg cgccgcgcgc gcacccaggc gcagccagtc gttggaccac
    11221 tggccggtga ccgaggccgc gtccgtttcc gagcggaagt tgtccctgtg gttgtcggtg
    11281 tcgcgcttct ggcccgtcgc gtcgagcgaa aaaccaccgc cggcgaccgt ggcggcggcg
    11341 cgcaggtcgc gcagcctgtg gctgccggcg ccggcataag ccgaggccgt gttgcgccgg
    11401 tccttgccgg tgccggcctt ggtggtgatg atgatcacgc cgccggtcgc gccttcgccg
    11461 taaagcacgg cgccgctgcc ccgcagcact tcgatgcgct cgaccgattc gatcgggatg
    11521 cccgcgagcc gcgtgccgcc gaggtcggcc tcgctcagcc gcagcccgtc gacgatgacg
    11581 acctggttgt tgtcggcggt ggcgccgaag ccgcgcaggt cgaggttgta ctcacccccg
    11641 ccatagaaat cctggcggcc gacgatgccc agtacccgca ccagcgcttc gttgacggtg
    11701 gcggcaccgg aggcctggat ctcgtcggcc gtgacgacgc tcacgcccag cggcagcgac
    11761 tgcgccggtt cggcaaagcg cgtggccgtg accacggttt ccttgagcgc gggagcttgc
    11821 gcgaaggagg acggaaaagc ggcggccagc gccaggcaca gcgcggatgc gcgtggtgat
    11881 acaaggagag cgttcatgat ggacgaaacg aatcaatacc cggaccagct tccccgccgg
    11941 tacagtgggc gtcacggccg cgcctttcga gggcgcagcc accgttgtcg gccggtatcc
    12001 gggctggcgg gtcaacttcc atcgccttcc caggcgcgct gttcagacgc acccagtggc
    12061 ttcatgtgat ggaagcggaa tctcctggga aattcatccg cttaccgttg cgggggcagc
    12121 gcaggttagg tccgccgtgg ccggcattcc ctcctgcttc ccgttgaact gcggcatgcg
    12181 aaccacaccg cgagcaccaa cgctttcatt ttaaggttga aaactgtcac aaaaccctac
    12241 aattggactt gtatgcccag tgccccccaa atcgaacaga ttgcggaacg cgtcgagcgc
    12301 ctgctcgtgc gttacgagga gctgcagcgc accaacacgc tgctggcggc acaagtggag
    12361 gtgctgacgc aggaacgcga ctcgctgaag tcgcggcttt cggccgcccg cgcgcgcgtc
    12421 gaggcactgc tcgagcggct gcccgagacc aagagcgcgg catgagccgc cgggagtgat
    12481 tcatttgagc ggcaacatga agcaactgga agtgcagatc atgggccaga gctacctgct
    12541 cgcctgcccc gacggcggcc agcagcggct gctggacgcc gtggagaaag tcgacaccgc
    12601 catgtgcagg atccgcgacg ccggcaaggt gaaggcacgc gaccgcatcg ccgttctcgc
    12661 ggcgctgaac ctggcgttcg atgtgaacga acgcgcgctg ccgttccagg gcacgggctt
    12721 cgctccctcc gagctggcgg ccaacgggaa tggcaacggc aatggcaatg gccatgcgca
    12781 cgatccgcgg ctggcccagc tgctcgcgcg actcgatagc gcactgggcg acgacggccg
    12841 attgatctag ggctgtcatg ccggactcga tccggcatcc acgttggcag gggacctgga
    12901 tcccgggtca agcccgggat gacagagggc atagttcacc tgttttccgt gctgtcaagt
    12961 caatttgctt tgccttccgc acaaagggta tttcgtcgcc gcgaagtgcc gcgcgagcgc
    13021 gcaagcccct acaatggccc cgtgtctgca gtgcatgccg ggctttatat ttccttgaac
    13081 caatgctcat tgagcccggg cttggaagaa tagccaggcg agcgtgatcg tctcgcgtca
    13141 gatgaaccca acgtctggct gacctcgccc acctgaaccc cggttcagga tgccggtccg
    13201 gtggcacttg cagacgcctt ttttcgcccc ccgcggtgaa atgacacacg ggccgaggcc
    13261 tcacgggcct gcgacgatcg tcggcgcccg gcgccagggc ggcgcgcaca acaacaaaga
    13321 tgctgctaga gccgcaactg attctcgaac tcgctctgct tggcagcgcc accggtttcc
    13381 tggcaggtct gctgggcata ggcggcggca tgctgatggt ccccttcatg accatcatcc
    13441 tcacggccaa gggctacccg ggcgagtaca cggtcaagat ggcggtggcc acatcgctgg
    13501 cgaccatctg cttcacatcc ctgtcgtcgg tgcgcgcgca cgcgcggcgc ggcgcggtgc
    13561 tgtggccgat cgtgcgcgtg ctggcgcccg gcatcctgct gggctcgctg gccggcgcgc
    13621 aactcgcggc ggcacttccg ggcaaggccc tgagcaccct gttcgcgatc ttcgtggctt
    13681 tctccgccac ccagatgttc ctggaccgca agcccaagcc cagccgcacc atgcccggcg
    13741 cgctgggcac cttcggcatg ggtgggctga tcggcttgct gtcttcgctg gtcggcgccg
    13801 gcggagcgtt cgtgtcggtg cccttcatga cgtggtgcaa cgtgaagatc cacgatgctg
    13861 tcggcacgtc cgccgcactc ggtttcccga tcgcgctggc cggcaccctg ggttatctgt
    13921 gggccggccg cgacctgccg cagatgccgg ccggctcagt gggttacctc tacctcccgg
    13981 ggctggcggt catctcgctc gccagcatca gcacggcgcc ggtaggcgcg cgtacggcgc
    14041 atcgcatgga catccggccg ctgcgaaaaa tattcgcgct ggtgctgtac attctcgcgg
    14101 cttactttct gctgcgctga gcccgtcgcc accgcgagca tccgctacag ctcgatcctg
    14161 atcgagtcac agggcggaca gatcgagggg ctcggacagg cccgacggcg gcaggaaaag
    14221 gggcgccggc gaattcgcgc gcgaacgcaa gctcctcagc tccgtttcct gtcgcggttc
    14281 gggccgaagc cgaccgtctg ctcgatgagg gcctcgcc
//
LOCUS       DASWIY010000258        11970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_902649, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000258 DASWIY010000000
VERSION     DASWIY010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902649"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VGA59_09540"
     CDS             complement(<1..747)
                     /locus_tag="VGA59_09540"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX9719961.1"
                     /translation="MTKTKGRNVVVVGTQWGDEGKGKLVDWLTEMAQGVVRFQGGHNA
                     GHTLVINGVKTALHLVPSGIMRPNVKCYIGNGVVLSAAKLFEEIEGLEKAGVEVRSRL
                     RISEACPLILPFHAALDIARETMREQGGSEKIGTTGRGIGPAYEDKIARRALRVQDLK
                     HPQRFGEKLKVVLDLHNFMLTKYLNAAPVDYQAVFDESMKHGELMRPMMADVSRELNE
                     AHLHGDNLLFEGAQGTLLDVDHGTYPYVTSS"
     gene            complement(774..1934)
                     /locus_tag="VGA59_09545"
     CDS             complement(774..1934)
                     /locus_tag="VGA59_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX9719962.1"
                     /translation="MSAWVLPDHIADVLPSEARHIEEIRRELLDTARAYGYELVMPPL
                     LEHLESLLTGTGEALDLQTFKLVDQLSGRMMGLRADTTPQVARIDAHLLNRQGVARLC
                     YCGPVLHTRPDRPHATREPLQFGAEIYGHAGLEADLEALLLALDCLKASGVDDLTVDM
                     ADARIVRSLLSGLPVDASVLARVHAALATKDASELRALTANFPAAAREGLLALVTLYG
                     DAKVLAEAEKALPTSAALREALATLRWLAGHLQGAKVSFDLADLRGYAYYSGARFAVY
                     TPGASDALVRGGRYDEVGAVFGRNRPAVGFSLDVKELVGVVAKPPLKAAIRAPWLDSG
                     EDAAALRKTISTLRSRGETVVCVLPGHESEVEEFQCDRELALAAGQWVVKAI"
     gene            complement(2067..2255)
                     /locus_tag="VGA59_09550"
     CDS             complement(2067..2255)
                     /locus_tag="VGA59_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEX9719963.1"
                     /translation="MDGDTFWAALALVLVLEGLLPFVSPGGWRHTFQRLLELRDGQLR
                     FFGLCSILIGLGLLWLVS"
     gene            complement(2258..3163)
                     /gene="hflC"
                     /locus_tag="VGA59_09555"
     CDS             complement(2258..3163)
                     /gene="hflC"
                     /locus_tag="VGA59_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX9719964.1"
                     /translation="MNRIGFMVTSALVVLALLSSMLFVVDQRQFGVVFALGQIKEVIL
                     EPGLNFKLPPPFQNVSYIDKRLLTLDSLDAEPMLTLEKQRVVIDWFVRWRIIEPSQYI
                     RNVGLDEAAGANQLNRVVRNAFQEEINRRTVRELLSSRREDVMAEVKREVLESVKGAK
                     PWGIDVIDVRITRVDYVDAITESVYRRMEAERKRVANELRSTGAAEGEKIRADADRQR
                     EVTVANAYRDAQKVKGEGDAQAAALYGEAFGRDPQFAQFYRSLEAYKASIGRKGDVLV
                     LDPNNSEFFKAFRGTGAGSAAPAKK"
     gene            complement(3176..4507)
                     /gene="hflK"
                     /locus_tag="VGA59_09560"
     CDS             complement(3176..4507)
                     /gene="hflK"
                     /locus_tag="VGA59_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEX9719965.1"
                     /translation="MNLHLPSLPWAQLKGRFRGMFNLNDPRWGRGDDKSEGNRPDKPE
                     GNGNGRGPNQGPPDLDELWRDFNRKLGGLFGGKGRRGDNGGGFGGGGGNFQPDMKSAG
                     IGAGLIAAVVVLIWLGTGFFIVQEGQQAVITQFGRYKSTVGAGFNWRLPYPVQRHEVV
                     FVTQIRSVDVGRDTVIKATGLRESAMLTQDENIVEIKFAVQYRLNDARAYLFESKNPA
                     DAVVQAAETAVREVVGKMRMDSALAEERDQIAPRVRTIMQTILDRYKVGVEVVGINLQ
                     QSGVRPPEQVQAAFDDVLKAGQERERAKNEAQAYANDVIPRAVGSASRLKEEAEGYKA
                     RIVAQAQGDAQRFRSVLVEYQKAPQVTRDRMYLEAMQQIYANIGKVMVDSRQGTNLLY
                     LPLDKLMQQSAQGAGAETPAATPATPPLSPAGLTNDARARDSSRGRERESR"
     gene            complement(4604..5776)
                     /gene="hflX"
                     /locus_tag="VGA59_09565"
     CDS             complement(4604..5776)
                     /gene="hflX"
                     /locus_tag="VGA59_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX9719966.1"
                     /translation="MSESLELPAAPTILVGVDLGLPHFDAELEELGLLAQTAGLAPVA
                     RVACRRKAPDAALFIGKGKADEIKALAQQTGATEILFDQALSPAQQRNLERHLDLPVN
                     DRTLLILEIFGQRARSHEGKLQVELARLQYVSTRLVRRWSHLERQTGGAGLRGGPGEK
                     QIELDRRMIGDAIRRTRERLEKVKKQRHTQRRQRERRGAFNISLVGYTNAGKSTLFNA
                     LVKARAYTADQLFATLDTTTRQLYLGEAGRSVSLSDTVGFIRDLPHGLIDAFQATLQE
                     AADADLLLHVVDAANPSYPEQIVEVQRVLHEIGAADVPQVLVFNKMDVLDPSQRPLQA
                     SDVFELEGIPVPRLFVSGRTSAGLPALRRQLVEELSQRGSAGVAARDNPVLHDTAI"
     gene            complement(5859..6107)
                     /gene="hfq"
                     /locus_tag="VGA59_09570"
     CDS             complement(5859..6107)
                     /gene="hfq"
                     /locus_tag="VGA59_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX9719967.1"
                     /translation="MSNKGQLLQDPFLNTLRREHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLRNTVTQMVYKHAISTIVPGRAVNFTTAESTEAGAT"
     gene            complement(6276..7613)
                     /gene="der"
                     /locus_tag="VGA59_09575"
     CDS             complement(6276..7613)
                     /gene="der"
                     /locus_tag="VGA59_09575"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX9719968.1"
                     /translation="MKPVMALVGRPNVGKSTLFNRLTKSRDAIVADFAGLTRDRHYGN
                     AKHGKLEFIVIDTGGFEPTAESGIYMEMAKQTRQAVAEADVVVFVLDARGGLTGQDHD
                     IAKYLRKLGKPTVLAANKAEGMTEGVQLSEFYELGLGEVHPVSAAHGQGIRSLVEEAF
                     ELLHLPDEEEEGEAQDTGVIKLAVAGRPNVGKSTLINTWLGEERLVAFDLPGTTRDAI
                     AVPFERAGQKFELIDTAGLRRRGKVFESIEKFSVVKTLQAIESANVVLLLLDATQGVT
                     DQDAHIAGYILESGRAVVLAVNKWDAVDPYGREQVQRQIESRLAFLKFAPIHFVSATK
                     RQGLGPVWAAIGQAHKSAMVKMPTPVLTRLLLEAVQFQSPKRAGMFRPKLRYAHQGGM
                     NPPVIVVHGNSLEHVTDAYKRFLEGRFRKEFNLIGTPLRIEMKTSRNPYADKE"
     gene            complement(7723..8889)
                     /gene="bamB"
                     /locus_tag="VGA59_09580"
     CDS             complement(7723..8889)
                     /gene="bamB"
                     /locus_tag="VGA59_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEX9719969.1"
                     /translation="MTGSRLLACARRTSALCAIALAAGLAGCGMMPSVFGGSTEKPKP
                     AELPPNASLIAVRPAWTARLGQVDLPLTVNVNGGTVAVASSDGTVAVLDAATGRDHWR
                     ASAGGPLAAGVGSDGKLVAVVSRANELVALAAGKPAWRQKMPAVSYTAPFVAGGRVFT
                     LTADRAVTAFDGQTGHKLWTQQRPGEPLVLRHSGVMLAVGDTLVVGLSGRMVGLDPLN
                     GRVRWEAPLATPRGINDVERLVDLVGGVSRVGDTVCARAFQASVGCVNAARGAVLWTK
                     PANGAEGVHGDDRLVFGTEADGKVLAWRRDTGDRVWVSDRLLHRGLTAPLALGRSVVV
                     GDSFGLVHLLSREDGTFLNRLTTDGSPIVAAPVLAGNTLVVVTRNGGVYGFVPE"
     gene            complement(8886..9575)
                     /locus_tag="VGA59_09585"
     CDS             complement(8886..9575)
                     /locus_tag="VGA59_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9719970.1"
                     /translation="MAKHLDLEEQEQLAEIKHFWNEYGNLITWVVIVVFGSIAAWNGW
                     QYWQRTQAAQAATLYDEVERAAQAGDLNRVERAFSDMRDKFGRTTYAQQAGLLAAKVL
                     VDQGKTDAAKSALGWVADEASDEGYQAVARLRLASILVEAKAYDEALKQLSADFPKEF
                     EPLVADRRGDVYNAQGKKAEAKAEYSKAFKGFDERTEYRRLVEVKLSALGVDTQQIGG
                     APTPAASGAKP"
     gene            complement(9625..10932)
                     /gene="hisS"
                     /locus_tag="VGA59_09590"
     CDS             complement(9625..10932)
                     /gene="hisS"
                     /locus_tag="VGA59_09590"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX9719971.1"
                     /translation="MNQKINAVKGMNDILPPDSARWEWLEEKVRALMARYAYRNIRTP
                     ILEPTALFVRGLGEVTDIVEKEMYSFEDRLNGEQLTLRPESTAGVVRAVVEHSMLYEG
                     GKRLYYMGPMFRHERPQRGRYRQFHQIGAEALGFAGPDVDAEIILLAHALWKELGLRD
                     VRLEINSLGQPEERKQHRAELTAYLETHAAVLDEDARRRQHSNPLRILDSKNPAMQVM
                     IEGAPKLIDFLGEASLAHFEGLKAMLEANGIDYRINPRLVRGLDYYSLTVFEFVTESL
                     GAQGTICAGGRYDYLIEQIGGKRAPAVGWALGVERVLELIKEHGAAIPTPVPDAFAVV
                     PDALAMPLVLKTLQALRAAGVSVQMHARPAAATPGAAAEGMGSMKSQLKKADASGARH
                     ALIFGADELAQGMVTVKALRDGTGAQVQRPLDEPAGWAGALKA"
     gene            complement(11014..>11970)
                     /gene="ispG"
                     /locus_tag="VGA59_09595"
                     /gene_synonym="gcpE"
     CDS             complement(11014..>11970)
                     /gene="ispG"
                     /locus_tag="VGA59_09595"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX9719972.1"
                     /translation="DFHYNGHRLLTEYPDCAQALSKYRINPGNVGKGDKHDRQFGQMI
                     EAAMRWGKPVRIGVNWGSLDQELLSQLMDENSARAEPWDAKSVMYEALITSAIGSARR
                     AEEMGMEGTQIILSCKVSGVQDLISVYRELAARCDYPLHLGLTEAGMGTKGTVASSVA
                     LGILLQEGIGDTIRVSLTPQPGESRTQEVVIASEIIQGLGLRSFVPSVTACPGCGRTT
                     STTFQELAKQIDDYLREQMPVWRAKYPGVEKMKVAVMGCIVNGPGESKHADIGISLPG
                     TGEAPAAPVFIDGRKAMTLRGDNIANEFHQIVENYIEKKYRA"
BASE COUNT         1812 a         4068 c         3933 g         2157 t
ORIGIN      
        1 gctggaggtc acgtagggat aggtgccgtg gtccacgtcc agcagcgtgc cctgcgcgcc
       61 ctcgaacagc aggttgtcac cgtgcaggtg cgcctcgttg agttcgcgcg agacgtcggc
      121 catcatcggt ctcatcagct cgccatgctt catcgactcg tcgaacaccg cctggtagtc
      181 caccggcgcc gcgttgaggt atttcgtgag catgaaattg tgcaggtcca gcaccacctt
      241 gagcttttcg ccaaagcgct ggggatgctt gaggtcctgc acgcgcagcg ccctgcgcgc
      301 gatcttgtct tcgtaggccg ggccgatacc gcggccggtg gtgccgatct tctcgctgcc
      361 gccctgctcg cgcatggttt cacgtgcgat gtcgagcgcc gcgtggaacg gcaggatcag
      421 cgggcaggcc tcgctgatgc gaaggcgcga gcgcacctcc acgcccgcct tttccaggcc
      481 ctcgatttcc tcgaagagct tcgcggccga cagcaccacg ccattgccga tgtagcactt
      541 cacgttcggc cgcatgatgc cgctgggaac gaggtgcagc gcggtcttca cgccgttgat
      601 caccagcgtg tggccggcgt tgtggccgcc ctggaagcgc accacgccct gcgccatttc
      661 ggtcagccag tcgaccagct tgcctttgcc ttcatcgccc cactgagtgc cgaccacgac
      721 cacgttgcgg ccctttgtct ttgtcatcgc gttggtttcc gggaaataaa aaatcagatg
      781 gccttgacga cccactggcc ggccgccagg gccagctcgc ggtcgcattg gaattcttcg
      841 acttcgctct cgtgacccgg cagcacgcag accaccgttt cgccgcggct gcgcagggtc
      901 gagatcgtct ttcgcagcgc ggccgcgtcc tctccgctgt ccagccaggg cgcgcggatg
      961 gccgccttca acggcggctt cgcgaccacg ccgaccagct ccttcacgtc caggctgaag
     1021 cccacggccg gccggttgcg gccgaacacc gcgcccactt cgtcgtagcg gccgccacgc
     1081 accagcgcgt cactggcgcc gggggtatag accgcgaagc gcgcgccgct gtagtacgcg
     1141 taaccgcgca ggtcggccag gtcgaagctc accttggcgc cttgcaggtg gcccgccagc
     1201 cacctcagcg tagccaacgc ctcgcgcagc gcagcgctcg tcggcagcgc tttctccgct
     1261 tcggcgagca ccttggcgtc gccataaagg gtcaccaacg ccagcagtcc ttcgcgcgcg
     1321 gctgcgggaa agttcgcggt gagcgcgcgc agctcgctcg catccttggt ggccagggct
     1381 gcatgcaccc gcgccagcac gctggcatcg acgggcagcc cgctcagtag cgagcgcacg
     1441 atgcgcgcat cggccatgtc caccgtcagg tcatcgaccc cgctcgcctt gaggcaatcg
     1501 agcgccagca gcagcgcttc caggtcggct tcgaggccgg catggccgta gatctcggcg
     1561 ccgaactgca gcggctcgcg cgtggcgtgc gggcgatccg ggcgggtgtg caagacgggg
     1621 ccgcagtagc acaggcgcgc caccccctgg cgattgagca ggtgcgcgtc gatgcgtgcg
     1681 acctggggcg tggtgtcggc gcgcaggccc atcatgcggc ccgaaagctg gtccaccagc
     1741 ttgaaggtct gcaggtccag cgcctcgccg gtaccggtga gcagcgattc caggtgctcc
     1801 agcagcggcg gcatcaccag ctcgtagccg taggcccggg cggtgtcaag caattcgcgt
     1861 cggatttcct cgatgtgccg ggcctctgaa ggcaagacat cggcgatgtg atccggcagg
     1921 acccaggcgg acatggtgag gggatgctgt taaaaacagt attttaccgg gcgcccgagc
     1981 ctgtcattgc ggaccccgga tcaagcctgc cccggacctg ctccgaggtc aagcccggga
     2041 tggcaatcct ctgggatgaa agatcttcag gagacaagcc agagcagccc caagccaatc
     2101 aggatgctgc acagcccgaa gaagcgcagc tgaccgtcgc gcagttccag cagccgctgg
     2161 aacgtgtggc gccagccacc cggcgacacg aagggcagca ggccttcgag caccagcacc
     2221 agtgccaacg cggcccagaa ggtgtcgccg tccaacttta tttcttggcg ggcgcggccg
     2281 accccgcgcc ggtgccacgg aaggccttga agaattccga attgttgggg tcgagcacca
     2341 acacatcgcc cttgcggccg atgctggcct tgtaggcttc caggctgcga tagaactgcg
     2401 cgaactgcgg gtcgcgccca aaggcttcgc catagagcgc ggcggcctgg gcatcgcctt
     2461 cgcccttgac cttctgcgcg tcgcggtagg cgttggccac tgtcacctcg cgctgacggt
     2521 cggcgtcggc gcggatcttt tcgccctcgg ccgcgccggt ggagcgcaac tcgttggcga
     2581 cgcgcttgcg ttccgcttcc atgcggcgat agaccgattc ggtgatggca tccacgtagt
     2641 ccacccgcgt gatgcgcacg tcgatcacgt cgatgcccca gggcttggcg cctttcaccg
     2701 attcgagcac ctcgcgcttg acctcggcca tcacatcctc gcgcctactc gaaagcagct
     2761 cgcgcaccgt gcggcggttg atctcctcct ggaacgcgtt gcgtacgacc cggttgagct
     2821 ggttggctcc cgcagcctcg tccaggccga cgttacggat gtactgggag ggctcgatga
     2881 tgcgccaacg cacgaaccag tcgatgacca cgcgctgttt ttccagggtc agcatcggct
     2941 cggcatcgag gctgtccagc gtcagcagtc gtttgtcgat gtacgagacg ttctggaacg
     3001 gcgggggcag cttgaagttc aggcccggct cgaggatgac ttccttgatc tggcccagcg
     3061 cgaagaccac gccgaattgg cgctggtcca ccacgaaaag catcgagctg agcagcgcca
     3121 gcactaccag cgccgacgtc accatgaatc cgattctgtt catatcctgt attccttagc
     3181 gcgattcgcg ttcgcgtcct cgcgagctgt cgcgagcgcg cgcgtcgttc gtgaggccgg
     3241 ccgggctgag cggcggagtc gcgggcgtgg cggccggcgt ctcggccccg gcgccttgcg
     3301 ccgactgctg catgagcttg tccagcggca ggtacagcag gttggtgccc tggcgcgagt
     3361 ccaccatcac cttgccgata ttggcgtaaa tctgctgcat cgcctcgagg tacatgcggt
     3421 cgcgcgtgac ctgcggcgcc ttctggtatt cgaccagcac cgagcggaag cgctgcgcat
     3481 cgccctgggc ctgggcaacg atccgtgcct tgtagccctc cgcttcttcc ttcaggcgcg
     3541 aggccgagcc cacggcccgt ggaatgacgt cgttggcata ggcctgggct tcgttcttgg
     3601 cacgctcgcg ctcctggccg gccttgagca cgtcgtcaaa agcagcctgg acctgctcgg
     3661 gcggccgcac gccgctttgc tgcaggttga tgcccaccac ttcgacgccg accttgtagc
     3721 ggtccaggat agtctgcatg atcgtgcgca cgcggggggc gatctggtcg cgctcctcgg
     3781 ccagcgccga atccatccgc atcttgccca cgacctcgcg cacagcggtt tcggcggcct
     3841 gcaccacggc gtcggcgggg ttcttgctct cgaatagata ggcgcgcgca tcgttcaggc
     3901 ggtactgcac cgcgaacttg atttcgacga tgttctcgtc ctgggtcagc atggccgatt
     3961 cgcgcaggcc ggtggccttg atcacggtgt cccggcccac atccacggac cggatctgcg
     4021 tgacgaacac cacctcatgg cgctggaccg ggtagggcag gcgccagttg aagccggcgc
     4081 ccaccgtgga cttgtagcgg ccgaactgcg tgatcacggc ctgctggcct tcctggacga
     4141 tgaagaaacc ggtacccagc cagatcagga ccaccaccgc ggcaatgaga cccgcgccta
     4201 tgccggcgct tttcatatcg ggctggaagt tgccgccacc gccgccgaag ccgccgccgt
     4261 tgtctccgcg gcggcccttg ccgccgaaca ggccgccgag cttgcggttg aaatcgcgcc
     4321 agagttcgtc caggtcgggc ggcccctggt tggggccccg gccgttgcca ttgccctcgg
     4381 gcttgtcggg ccgattgcct tcggacttgt cgtcgccgcg cccccagcgc ggatcgttca
     4441 ggttgaacat gcccctgaac cggcctttca gctgcgccca cggcaggctg ggtaaatgga
     4501 ggttcatgct attggtgatc tctttctggt cagtgaccgt tcgccgcccc caagggcggc
     4561 gacgaagtga gcgtggagtg ctccatcagc gcattgtgcc caatcagatg gccgtgtcgt
     4621 gcaatacagg attatcccgg gcagccacgc cggcggaacc gcgctggctc aattcttcga
     4681 ccagttgccg gcgcagcgcc ggcaggccgg cggaggtgcg cccgctgacg aacaggcgcg
     4741 gcaccggaat gccttcgagc tcgaacacgt cgctggcctg gagcgggcgc tggctgggat
     4801 ccagcacgtc catcttgttg aacaccagca cctgcggcac gtcggcagcg ccgatttcat
     4861 gcagaacgcg ctgcacttcg acgatctgtt ccgggtaact ggggttcgcg gcgtccacca
     4921 catgcagcag caggtcggca tcggcggcct cctgcaaggt ggcctggaag gcgtcgatca
     4981 agccgtgcgg caggtcgcgg atgaaaccga ccgtgtccga caaggacacc gagcggccgg
     5041 cctcgcccag atagagctgg cgcgtcgtgg tatccagcgt ggcgaacagc tggtcggcgg
     5101 tataggcacg ggccttgacc agcgcgttga acagggtgga cttgccggcg ttcgtgtagc
     5161 cgaccaggga gatgttgaag gcgccgcgcc gttcgcgctg gcggcgctgc gtgtggcgct
     5221 gcttcttgac cttctccagc cgctcgcgcg tgcgccggat cgcgtcgccg atcatccgcc
     5281 tgtccagttc gatctgcttt tcacccgggc cgccgcgcag gcccgcaccg cccgtctggc
     5341 gttccaggtg ggaccacctt cggacaagcc gggtgctgac atactggagc cgggccagct
     5401 cgacctgcag cttgccctca tggctgcgcg cacgctggcc gaagatttcg aggatgagca
     5461 aggtgcggtc gttgaccgga aggtcgaggt ggcgctccag gttgcgctgc tgggccgggc
     5521 tcagggcctg atcgaacagg atctcggtcg cacctgtctg ctgcgccagc gccttgatct
     5581 cgtccgcctt gcccttgccg atgaacagcg ccgcgtcggg cgccttgcgt ctgcacgcga
     5641 cgcgcgcgac cggggccagg ccggcggttt gcgcgagcag gcccagctct tcgagctcgg
     5701 cgtcgaaatg gggcaggccc aggtccacgc ccacaaggat cgtgggggcg gctgggagtt
     5761 cgagcgattc ggacaaactc aagaccaggg gttcaagaag gcgactatag gatcggcgag
     5821 gccgcgcaac cgttccagcg cctggagcgc ctcgttcttc aagtggcgcc ggcttcggtg
     5881 ctttcggccg tggtgaagtt cacggcccgc ccgggcacga tggtggagat ggcgtgcttg
     5941 taaaccatct gggtcacggt gttgcgcagc agcacaacgt attgatcgaa agactcaatc
     6001 tggccttgca gcttgatccc gttgacgagg taaatcgaca ccggcacatg ctcgcggcgc
     6061 aaggtgttga gaaatgggtc ttgtagaagc tgccctttgt tgctcacgat attctccgtg
     6121 ttccgcgttc ggaaatgttg ttggaaactg caccttacca cagcaagagt gccgcgtaag
     6181 taggccgatg ccgataccac tgcagctggg tgcggatttt gctacttcaa gcgccgggct
     6241 cgaaaatgat ttgccggcgc ggcacgcggg gcggctcatt ccttgtcggc gtaagggttg
     6301 cgcgaggtct tcatctcgat gcgcagcggc gtgccgatca gattgaattc cttgcgaaag
     6361 cggccctcga ggaagcgttt gtaagcgtcg gtcacgtgct cgagggaatt gccgtgcacg
     6421 acgatcaccg gcgggttcat tccgccctga tgggcgtagc gcagcttggg ccggaacatg
     6481 ccggcgcgct tgggcgactg gaactgcacg gcctccagca ggaggcgggt gagcaccggc
     6541 gtgggcatct tcaccatggc cgacttgtgg gcctggccga tcgcggccca gaccggcccc
     6601 aggccctggc gcttcgtggc cgagacgaaa tggatgggcg cgaacttgag gaaggccagc
     6661 cgcgactcga tctgccgctg cacctgttcc cgcccgtacg ggtccacggc atcccacttg
     6721 ttgacggcca gcaccacggc ccggccgctc tcgaggatgt aaccggcgat gtgcgcatcc
     6781 tggtcggtca cgccctgggt ggcgtccagc agcagcagca cgacgttggc cgactcgatc
     6841 gcctgcaagg tcttgaccac cgaaaacttc tcgatcgact cgaacacctt gccccggcgc
     6901 cgaaggcccg cggtatcgat cagttcgaac ttctgcccgg cccgctcgaa cggcacggcg
     6961 atcgcgtcgc gggtcgtgcc cggcaggtcg aacgcgacca ggcgttcctc gccgagccag
     7021 gtattgatca gcgtggattt gccgacattg ggccgcccgg ccaccgccag cttgatgacg
     7081 cccgtgtcct gcgcctcgcc ctcttcctcc tcgtcgggaa ggtgcaacag ttcgaacgcc
     7141 tcttcgacca ggctgcggat gccttgtccg tgcgccgccg aaaccggatg cacctcgccc
     7201 aggccaagct cgtagaactc gctcagctgc accccttcgg tcatgccctc ggccttgttg
     7261 gccgccagca ccgtcggctt gcccagcttg cgcaggtatt tggcgatatc gtggtcctgg
     7321 cccgtgagcc cgccacgggc gtcgagcacg aagacgacca cgtcggcttc ggccaccgcc
     7381 tgccgggtct gcttggccat ctccatgtag atgccgctct cggctgtggg ctcgaagccg
     7441 ccggtatcga tgacgatgaa ttcgagcttg ccgtgtttcg cgttgccgta atgccggtcg
     7501 cgcgtgaggc cggcaaagtc cgccacgatc gcatcgcgcg acttcgtcag ccggttgaac
     7561 agcgtcgact tgccgacgtt ggggcggccc accagggcca tcactggttt catttcacgt
     7621 aacgatcgcc gccggggacc tcgagtgccg ccgggccgcc ccaaggcacg agggtccctc
     7681 ggggcggcgc agcggcctta gccgcaagcg tgggggccgc attcattccg gcacgaagcc
     7741 gtacacgccg ccattgcgtg tcaccaccac cagcgtgttg ccggccagca ccggggcggc
     7801 gacgatcggc gagccgtccg tggtcaggcg gttgaggaac gttccgtctt cacgcgacag
     7861 cagatgcacc aggccaaagc tgtcgccgac cacgacggaa cggcccaggg ccagcggcgc
     7921 ggtgagcccg cgatgcagca gcctgtcgct gacccacacc cggtccccgg tgtcgcggcg
     7981 ccaggcgagc accttgccgt cggcctcggt gccgaatacc agccggtcgt cgccgtgcac
     8041 accctcggcg ccgttggccg gcttggtcca cagcacggcg ccccgggccg cattgacgca
     8101 gcccacgctg gcctggaagg cccgggcgca caccgtgtct cccacccggc tgacgccgcc
     8161 caccaggtcg accagccgtt cgacatcgtt gatgccgcgc ggggttgcca gcggcgcttc
     8221 ccagcgcaca cggccattga gcgggtcgag ccccaccatg cggcccgaca ggcccaccac
     8281 cagcgtgtct cccaccgcca gcatgacgcc ggagtggcgc agcaccagcg gttcgccggg
     8341 acgctgctgg gtccagagct tgtggccggt ctggccatcg aaggccgtga cggcgcggtc
     8401 ggccgtcagt gtgaagacgc ggccgcccgc cacgaatggc gcggtgtagg aaacggcggg
     8461 catcttctgg cgccaggccg gtttgccggc cgcgagcgcc accagttcat tggcccggct
     8521 caccaccgcg acgagcttgc catcgctgcc gacgccggcc gccagcggcc cgccggcact
     8581 ggctcgccag tggtcgcggc cggtcgcggc gtcgaggacc gccaccgtac cgtcgctgct
     8641 ggccacggcg accgtgccgc cgttgacgtt gaccgtcagc ggcaggtcga cttgtcccag
     8701 ccgggccgtc caggccggcc gtaccgcgat cagcgacgcg ttgggcggca gttccgcggg
     8761 cttgggcttt tcggtgctgc cgccgaagac ggacggcatc atgccgcagc cggccaggcc
     8821 ggccgccagg gcgatggcgc aaagggccga tgtcctgcgc gcgcaggcca ggaggcgcga
     8881 cccggtcatg gcttggcccc cgaagcggcc ggtgtcggcg cccccccgat ctgctgggtg
     8941 tcgacaccca gtgcgctgag cttgacctcg accaggcgcc ggtactcggt ccgctcatcg
     9001 aaacccttga atgccttgga atactcggcc ttggcctccg ccttcttgcc ctgcgcgttg
     9061 taaacgtccc cgcgccggtc ggccaccagc ggttcgaact ccttcgggaa atcggcggac
     9121 aactgcttga gggcctcgtc gtaggccttg gcttccacca ggatactggc caggcgcagc
     9181 cgcgccacgg cctggtaacc ctcgtccgac gcctcgtcgg ccacccagcc gagcgcggac
     9241 ttcgccgcgt cggtcttgcc ttggtcgacc aggaccttgg cggccagcag gccggcctgc
     9301 tgcgcgtagg tggtgcggcc gaacttgtcc ctcatgtcgg agaacgcccg ctccacccgg
     9361 ttcaggtcgc cggcctgcgc cgcgcgttcg acctcgtcgt acagggtcgc ggcctgggcg
     9421 gcctgggttc gttgccaata ctgccagccg ttccaggcgg cgatggagcc gaagacgaca
     9481 atcaccaccc aggtgatgag gttgccgtac tcgttccaga agtgcttgat ctcggcaagc
     9541 tgctcctgtt cttcgaggtc gaggtgtttg gccattgtgt tgttttgtcc tgtcgcgagc
     9601 ctgcgattgt agtgagggga ccgatcaggc cttgagggcg cccgcccatc cggccggctc
     9661 gtccagcggc cgctggacct gcgcacccgt gccgtcgcgc agcgccttga cggtgaccat
     9721 gccctgcgcc agttcgtcgg cgccgaagat cagcgcatgg cgcgccccgc tggcatcggc
     9781 cttcttcaac tgggatttca tgctgcccat gccttcggcc gcggcgccgg gcgttgccgc
     9841 cgccggcctg gcgtgcatct gcacgctcac gcccgcggcg cgcagcgcct gcaatgtctt
     9901 gagcaccagc ggcatcgcca gggcgtcggg caccaccgca aaggcatccg gcaccggcgt
     9961 cgggatcgcg gcgccgtgct ccttgatgag ttccagcacg cgctccacgc ccaacgccca
    10021 gcccaccgcc ggcgcgcgct tgccgccgat ctgctcgatc aggtagtcgt aacgcccgcc
    10081 ggcgcagatc gtgccttgcg cgccgaggct ttcggtgacg aattcgaaca ccgtcaggct
    10141 gtagtagtcc aggccgcgca ccaggcgcgg gttgatgcga tagtcgatgc cgttggcctc
    10201 gagcatggcc ttcaggccct cgaagtgagc gagcgaggcc tcgccgagga agtcgatcag
    10261 ctttggcgcg ccctcgatca tgacctgcat ggcggggttc ttgctgtcca ggatccgcaa
    10321 aggattgctg tgctggcggc ggcgcgcgtc ctcgtccagc acggcggcgt gcgtttccag
    10381 gtaggcggtc aactcggcgc gatgctgctt gcgctcctcg ggctgcccca ggctgttgat
    10441 ttcgaggcgc acgtcgcgca agcccagttc cttccacagc gcgtgggcca gcaggatgat
    10501 ttccgcgtcc acgtcggggc cggcgaagcc cagcgcttcg gcgccgatct ggtggaactg
    10561 gcggtagcgc ccgcgctggg gccgctcgtg ccggaacatg gggcccatgt agtacaggcg
    10621 cttgccgcct tcgtaaagca tggaatgttc gaccaccgcg cgcaccacgc cggctgtgct
    10681 ttccgggcgc agcgtcagct gctcgccgtt gagccggtcc tcgaaggaat acatctcctt
    10741 ctcgacgatg tcggtgactt cgcccaggcc gcgcacgaac agcgccgtcg gctcgaggat
    10801 cggtgtgcgg atgttgcggt aggcgtagcg cgccatcaac gcgcgcactt tttcctccag
    10861 ccactcccag cgcgccgagt caggcggcaa tatgtcgttc atgcctttga cggcattgat
    10921 tttttggttc atagcggcct agggctaggg gctaggggct cggggctagg gaacagccga
    10981 atgcattctc cctaacccct agtcccctag gccctaggcc ctgtatttct tctcgatata
    11041 attttcgacg atctggtgga actcgttggc gatgttgtcg ccccgaaggg tcatggcctt
    11101 gcgcccgtcg atgaagaccg gcgcggccgg tgcctcgccg gtgccgggca ggctgatgcc
    11161 gatgtcggcg tgcttgctct cgccagggcc gttgacgatg cagcccatca cggccacctt
    11221 catcttctcg acgccagggt acttggcgcg ccacaccggc atctgctctc gcaggtagtc
    11281 gtcgatctgc ttggccagtt cctggaaggt cgtgctggtg gtgcgcccgc agccggggca
    11341 ggcggtgacg ctgggcacga agctgcgcag gcccaggccc tggatgattt ccgaggcgat
    11401 taccacttcc tgcgtgcgcg actcgcccgg ctggggcgtg agcgagaccc ggatggtgtc
    11461 gccgatgcct tcctgcagca ggatcccgag cgcgacgctg gacgccaccg tgcccttggt
    11521 gcccatgccc gcttcggtca ggcccaggtg caaggggtag tcgcagcgcg cggccagctc
    11581 ccggtacacc gagatcaggt cctgcacgcc gctcaccttg cacgagagga tgatctgggt
    11641 gccctccatg cccatctcct cggcgcgccg ggccgaaccg atggccgacg tgatcagggc
    11701 ctcgtacatc accgatttcg cgtcccacgg ctcggcccgg gcgctgttct cgtccatcag
    11761 ctgcgacaac agctcctggt ccaggctgcc ccaattcacg ccgatgcgca cgggcttgcc
    11821 ccagcgcatc gccgcctcga tcatctggcc gaactgccgg tcgtgcttgt cgcccttgcc
    11881 gacgttgccg gggttgatcc ggtacttgga cagcgcctgc gcacagtcgg ggtactcggt
    11941 gagcaggcgg tggccgttgt agtggaagtc
//
LOCUS       DASWIY010000259         5984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1660487, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000259 DASWIY010000000
VERSION     DASWIY010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660487"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VGA59_09600"
     CDS             <1..912
                     /locus_tag="VGA59_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9719973.1"
                     /translation="HVYTDGSVQVNHGGTEMGQGLNTKVAQLVADELGVPFERVRSTA
                     ADTSKVPNASATAASSGTDLNGRAAQYAARNVRDNLAAFVSGLDGCGAGAVVFRGGEV
                     ISPKATRTFDEVVKAAYANRIQLWSDGFYRTPKIHYDKATLTGRPFYYFSYGAACSEV
                     AIDTLTGESRLLKVDILHDVGTSINPAIDIGQIEGGFIQGVGWLTNEQLVWSDKGELA
                     THAPSTYKIPATGDVPEHFNIELWPEPNREDNVGGSKAVGEPPLMLAIGVWEALREAV
                     AAAKGDGRPVRMDAPATPENVLRSLKH"
     gene            1040..2323
                     /locus_tag="VGA59_09605"
     CDS             1040..2323
                     /locus_tag="VGA59_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEX9719974.1"
                     /translation="MSNDWKFETLLVHAGYSPDPTTHAVVPPIAQTVAYAFDSAQHGA
                     DLFDLKVAGNIYTRIMNPTQDVLEQRLAALEGGVAALAMASGMAAITASIQTIAETGD
                     NIVSSSTLYGGTYNLFAHTFPQQGIEVRFADPSKPEDFAALIDARTKAVFCESIGNPL
                     GNVTDLAALAKVAHDHGVPLIVDNTVPSPYLCRPFEHGADIVVHSLTKYLGGHGNSIG
                     GAIVDSGKFPWATHKARFKRLNEPDVSYHGVVYTEALGPAAYIGRARVVPLRNMGGAI
                     SPFNAWLIIQGIETLALRMDRICDNTLAVAKHLKASPKVTWVNYAGLPEHAEHALARK
                     YLGGRASGILTFGIEGGRKSGERFLDALKLFTRLVNIGDVRSLATHPASTTHRQLAAD
                     ELAKAGVSEETVRLSVGIEHIDDLRADLEQALSAA"
     gene            complement(2368..2691)
                     /locus_tag="VGA59_09610"
     CDS             complement(2368..2691)
                     /locus_tag="VGA59_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX9719975.1"
                     /translation="MNGYQITFFTQQDHRHKGKPVGEWLVHLAKELGLRGATLMAAGE
                     GFGHHGRIHSAHFFELADQPLEVVMALSAEETDRLFERLKGEGVHVFYVKTAVEFGTL
                     GEPDS"
     regulatory      complement(2754..2827)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            2966..3931
                     /locus_tag="VGA59_09615"
     CDS             2966..3931
                     /locus_tag="VGA59_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="reductase"
                     /protein_id="HEX9719976.1"
                     /translation="MSNEPNPPQAELDALLGFAPDADCPIPYMKRTREWYLALGYGNP
                     YRWAHYSEVPFCRPAKPLSKCTATLITTAAPYQPGKAPLAPGAAYNAAAKFYAVYSGD
                     TGTDPALRVDHVAIDRKHTSMEDSGCWFPLPALRRAAAAGRIGALAHRFHGVPTHRSQ
                     RHTLAVYCPEILSRCLEDGVDVAVLVPNCPVCHQTLSLVARHLEGSGIATVVMGCAKD
                     IVEHVGVPRFLFSDFPLGSAAGRPHDPDSQASTLELALRVLESSPGPRTTMQSPLRWS
                     ESHDWKLDYSNVERVTPEELALLRTEFDRAKAVAKSVRDSTGRTE"
     gene            complement(4110..5141)
                     /locus_tag="VGA59_09620"
     CDS             complement(4110..5141)
                     /locus_tag="VGA59_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX9719977.1"
                     /translation="MTNDSPTVPAAPELDRFLHLQGLATEAEQATWTPLAGGVSSDIW
                     RVDLPGRTLCVKRALPKLRVGSDWWAPVSRNRYEWEWLRLAARIVPRSVPQPLAHDPD
                     LGLFAMSFLPAADYPLWKRQLLAGNIDVETAARVGHLLATLHNATAGRPEVAAAFDTT
                     DNFRALRLDPYLLATAVRHPDLEPALVALVERTAQARIALVHGDVSPKNILVGADAPV
                     LLDAECAWFGDPAFDIAFCLNHLLLKSVLMPKRLDELLRAFDAMATSYLDEVRFEKRA
                     GLEARAAGLLPALALARIDGKSPVEYLVGRGSEQDLVRRLSRPLIAQPVNSLTTVVVQ
                     WRAEVTGAR"
     gene            complement(5128..>5984)
                     /gene="eno"
                     /locus_tag="VGA59_09625"
     CDS             complement(5128..>5984)
                     /gene="eno"
                     /locus_tag="VGA59_09625"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012341.4"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9719978.1"
                     /translation="MMNVLSGGLHAGRGMDVQDFLVIPMRAGSVAEAVHLLARVRTAA
                     YDVARGRGLPTLLADEGGLSPGLESGRLALRMLMQCIEKAGLTPGKDIGIAIDVAAST
                     LKGKDGRYVFAKEGVERSSAELIEMLEGWSEDFPVVSIEDGLDEEDWSGWARLTQTLG
                     DKVQLVGDDLFATNASRLARGIEGGVANGVLVKANQNGTLSGTLDVIAQARQAGYAAI
                     VSARSGETEDAFMSDIAVGSAAGQIKIGSLRTSSTVAKYNQLLRIEEHSGADYAGVRH
                     LRGWNDQR"
BASE COUNT         1049 a         2062 c         1888 g          985 t
ORIGIN      
        1 catgtgtaca ccgacggcag cgtccaggtg aaccacggcg gcaccgagat gggccagggc
       61 ctgaacacca aggtggcgca gctcgtggcc gacgagctgg gcgtgccctt cgaacgcgtg
      121 cgctccacgg cggccgacac cagcaaggtg cccaatgcgt cggccacggc cgcctcgagc
      181 ggcaccgacc tgaacggccg cgccgcacag tacgcggccc gcaacgtgcg cgacaacctg
      241 gccgcgttcg tcagtgggct ggacggctgc ggcgcgggcg ccgtcgtctt tcgcggcggc
      301 gaggtgatct cacccaaggc cacccgcact ttcgacgagg tggtgaaggc cgcctatgcc
      361 aaccgcatcc agctgtggag cgacggcttc taccgcacgc ccaagatcca ctacgacaag
      421 gccacgctca ccggccggcc gttctactac ttcagctacg gcgccgcctg cagcgaggtg
      481 gcgatcgaca ccttgaccgg cgagagccgc ctgctgaagg tggacatcct gcacgacgtg
      541 ggcacgagca tcaatccggc catcgacatc ggccagatcg agggcggctt cattcagggc
      601 gtgggctggc tcaccaacga gcagctggtc tggagcgaca agggcgagct cgccacccac
      661 gccccgagca cgtacaagat cccggcgacg ggcgatgtgc ccgagcattt caacatcgag
      721 ctctggcccg agccgaaccg cgaggacaac gtgggcggca gcaaagccgt gggcgaaccg
      781 ccgttgatgc tggcgatcgg tgtctgggag gccctgcgcg aagcggtggc agcggcgaag
      841 ggtgacggcc ggccggtccg gatggatgcg ccggctacgc cggagaatgt gctgcgctcc
      901 ttgaagcact gacggtccgg ggggcaagcg tcattcagat gctggccctt tcttgacaaa
      961 gaccgccgtg cacccggtgg gcgacttgca ttcggcaata atccagccga tcccaaacca
     1021 ccattagaaa gagacaccca tgtcgaacga ctggaaattc gaaaccctct tggttcacgc
     1081 cggctattca cccgacccga cgactcatgc cgtcgtgcca ccgatcgccc agacggtggc
     1141 ctatgcgttc gacagcgcac agcatggcgc cgacctgttc gacctgaaag tcgcgggcaa
     1201 tatttatacg cggatcatga atccgaccca ggacgtgctg gagcagcgcc tggccgcgct
     1261 tgaaggcggt gttgccgcgc tcgccatggc ttcgggcatg gccgcgatca ccgcctcgat
     1321 ccagaccatc gcggaaacgg gcgacaacat cgtttcgtcg agcacgcttt acggcggcac
     1381 ctacaacctg ttcgcccaca cgttcccgca acaggggatc gaggtgcgct ttgccgaccc
     1441 gtccaagccg gaggactttg ccgcgctcat cgatgcgcga accaaggcgg tcttctgcga
     1501 atcgatcggc aatcccctgg gcaacgtgac ggacctcgcc gcgctggcga aggttgccca
     1561 tgaccacggc gtgccgctca tcgtggacaa cacagtcccc agcccctacc tgtgccgtcc
     1621 gttcgaacac ggcgcggaca tcgtcgtgca ttcactgacc aagtatttgg gcggacacgg
     1681 caacagcatc ggcggcgcga tcgtggacag cggcaagttt ccctgggcca cgcacaaggc
     1741 acgcttcaaa cggctgaacg agcccgacgt cagctaccac ggcgtggtct acacggaggc
     1801 actcggcccg gcggcttata tcgggcgcgc ccgcgtcgtg cccctgcgca acatgggggg
     1861 agcgatatcg cccttcaatg cgtggctgat cattcaaggc atcgaaacgc tggcgctgcg
     1921 catggatcgg atctgcgaca acacgctggc agtggcgaaa cacctgaaag ccagccccaa
     1981 ggtcacgtgg gtgaactatg ccggcctgcc ggaacacgcc gagcacgcgc tggcgcgcaa
     2041 gtacctgggc gggcgcgcct cgggcatcct gaccttcggc atcgaaggcg gacgcaagtc
     2101 cggcgaacgc ttcctcgatg cgctcaagct gttcacgcgc ctggtcaaca tcggggacgt
     2161 acgctcgctg gccactcacc cggcctccac cacgcatcgc cagctggcgg ccgacgagct
     2221 ggccaaggcc ggcgtgtcgg aagagacagt gcgcctttcg gtaggcatcg agcacatcga
     2281 cgatcttcgg gccgacctgg aacaggcgct gtccgctgcc tgaagcgcgc ggcgaaaaca
     2341 ggtgggcggg ccgcccaccg acggcggtca cgaatccggt tcacccagcg tgccgaactc
     2401 aaccgccgtc ttgacataga agacgtgcac cccctcgccc ttcagccgct cgaacaggcg
     2461 gtcggtctcc tccgcgctca aggccatcac cacttccaga ggctggtccg ccaattcaaa
     2521 aaaatgggcc gaatgaatgc gcccgtgatg cccgaagcct tcgcccgccg ccatgagggt
     2581 cgcgccgcgc aggcccagct cctttgccag gtgaaccagc cattcaccca cgggcttgcc
     2641 cttgtgccgg tggtcctgtt gtgtgaagaa cgtgatttgg tagccgttca taaccgatcc
     2701 tttcgaggca cggtggctcg gacaggccat tgcctgcgtg cccgagtcac gggtctatcg
     2761 caggcatcgt cagcagcaca tcaacggtgc atgcggttca acgaggaatg ccatctcctg
     2821 gtgaatctca actctagcag accatgagtg tcggcggcgt gttcagcacg tccgcccccg
     2881 gccgcggcgg cgtctttcag ctccggccct ttgctgttgt cggcaaatct gattgaattg
     2941 agtttgctgc caggagtctg acgacatgag caacgaaccc aatcctccac aagccgagct
     3001 ggacgcgttg ctgggtttcg cgccggacgc cgactgcccg attccgtaca tgaagcgcac
     3061 gcgcgagtgg tacctggcgc tgggctatgg aaatccgtac cgctgggcac actattcgga
     3121 ggtgccgttc tgccgtccgg ccaaaccgct ttcgaagtgc actgcgaccc tcatcacgac
     3181 ggccgcacca tatcagccgg gcaaagcgcc gctggcgccg ggcgcagcct acaacgcggc
     3241 ggcgaagttc tacgcggtct attcaggcga caccggcacc gaccctgccc tgcgggtgga
     3301 ccacgtcgcc atcgaccgca agcacacgtc gatggaagac agcggctgct ggtttccgct
     3361 gcctgcgctg cgccgcgcgg cggcagcggg ccgcatcggc gcactggcgc atcgtttcca
     3421 tggcgtgccg acccaccgca gccagcgcca tacgctggcg gtctattgcc ccgagatcct
     3481 gtcgcgttgt ctcgaggacg gggtggacgt ggcggtgctg gttccgaatt gcccggtatg
     3541 ccaccagacg ctgagcctgg tggcgcggca cctggagggc agcggcatcg cgacggtcgt
     3601 catgggctgc gcgaaggaca tcgtcgaaca cgtgggtgtt ccgcgtttcc tgttcagcga
     3661 ctttccgctg ggcagcgcgg ccggccgccc gcacgacccc gattcgcagg cgtcgacgct
     3721 ggagcttgca ctgcgcgtgc tggaatcgtc gcccggcccg cgcaccacca tgcaatcgcc
     3781 gctgcgctgg agcgagtcgc atgactggaa actggactac tccaatgtcg aacgcgtgac
     3841 ccccgaggaa ctggcgttgc tcaggacgga gttcgaccgc gcgaaagcgg tggcgaagtc
     3901 ggttcgggat tcgaccggca ggactgagtg agccgatgcc tgtcgtgccg gatttgattg
     3961 cgttccaaca tggattgcgg ggccgggccg tagcgtgatg cctcggtacg tgcatagcgt
     4021 aggcccggca ttgggctcgc tgcactggac agctgcgcgc cccgggcgtt ccgcgagccc
     4081 agcccgcaat gacaacatcc tgatccccat caccgcgcgc ctgtaacttc cgcccgccat
     4141 tggacaacga ccgtcgtcaa cgaattcacc ggctgcgcga tcaagggccg tgagagcctt
     4201 ctcacgaggt cttgttccga accacgtccc accaggtact cgaccggcga cttgccgtcg
     4261 atgcgcgcga gcgccaaggc cggcagcagg ccggccgcgc gcgcctccag gccggcgcgt
     4321 ttttcgaatc gcacctcatc gagatagctc gtcgccatgg cgtcgaatgc ccgcagcagc
     4381 tcgtccaggc gtttcggcat caagaccgac ttgagcagga ggtggttcag gcaaaaggcg
     4441 atatcgaaag ccgggtcccc gaaccacgcg cattcggcat ccagcaacac cggcgcatcg
     4501 gcgcccacca ggatgttctt cgggctgacg tcgccgtgaa ccagcgcgat cctggcctgc
     4561 gccgtgcgtt ccaccagcgc caccagcgcc ggctcgaggt ccggatggcg cacggcggtg
     4621 gccagcaagt aggggtccag cctcagcgcg cggaagttgt ccgtcgtgtc gaatgcggcg
     4681 gcgacttccg gccggccggc cgttgcgttg tgcagcgtgg ccaacaggtg cccaacccgt
     4741 gccgccgtct ccacgtcgat gttgccggcg agcaattgcc gcttccacag gggatagtcg
     4801 gcggccggca ggaaggacat cgcgaacagc cccaggtccg ggtcgtgcgc cagcggctgg
     4861 ggaaccgagc gcggcacgat ccgggcggcc agccgcagcc actcccactc gtacctgttg
     4921 cgcgagaccg gcgcccacca gtcggagccg acccgcagct tcggcagcgc gcgcttcacg
     4981 cagagggttc gccccggcag gtcgacccgc cagatgtcgg aagacacgcc gccggccagc
     5041 ggcgtccagg tggcctgctc cgcctccgtg gccaggccct gcagatgcag gaacctgtcg
     5101 agctccggcg ctgcgggaac cgtcgggcta tcgttggtca ttccagcctc gcaggtggcg
     5161 cacgcccgcg taatccgccc cgctgtgttc ctcgatccgc agcagctggt tgtacttggc
     5221 caccgtgctc gacgtgcgca gcgaaccgat cttgatttgc cccgccgcgc tgcccacggc
     5281 aatgtcggac atgaacgcgt cttcggtctc gccggatctc gcggacacga tcgccgcgta
     5341 gcccgcctgc ctggcctgag caatcacatc gagcgtgccg ctgagcgtgc cgttctgatt
     5401 ggccttcacg agcacgccgt tggcgacacc cccctcgatg ccgcgcgcca gccggctcgc
     5461 attggtcgcg aacaggtcgt cgcccacgag ttgaaccttg tcgccgagcg tctgggtgag
     5521 ccgggcccag ccgctccagt cttcctcgtc cagtccgtcc tcgatcgaga ccactggaaa
     5581 atcttcgctc cagccctcga gcatttcgat caattcagcg gagctcctct ccacaccttc
     5641 cttcgcgaag acgtaccggc catctttgcc cttgagggtg ctcgccgcaa cgtcaatggc
     5701 gatgccgatg tctttccccg gagtcaagcc ggctttttcg atgcattgca tcagcatccg
     5761 cagcgcaagg cggccggatt cgagtccagg gctcaagccc ccttcgtcgg ccagcagcgt
     5821 cggcaggccg cggccccgcg cgacatcgta tgctgccgtg cgcacccgcg ccagcaggtg
     5881 gaccgcttcg gctacgctgc ccgcccgcat cgggatcacg aggaagtcct gcacatccat
     5941 gccgcggccc gcgtgcaaac caccgctcaa gacattcatc atcg
//
LOCUS       DASWIY010000260         7217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_904891, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000260 DASWIY010000000
VERSION     DASWIY010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904891"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..99)
                     /locus_tag="VGA59_09630"
     tRNA            complement(9..99)
                     /locus_tag="VGA59_09630"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Ser,seq:gga)
     gene            complement(144..2222)
                     /locus_tag="VGA59_09635"
     CDS             complement(144..2222)
                     /locus_tag="VGA59_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719979.1"
                     /translation="MRNPVPTTLPQIAEREKANILVVDDLPEKHIVFRSILEELGQNI
                     VSARSGQEALRFILEKEFAVILLDVNMPDIDGLETASLIRQYKKSAQTPIVFITAYVD
                     ERQAKRGYALGAVDYIPSPVVPEVLRSKVRVFVELYRMNQQLQVQAAQREALARSEAA
                     RDAAESAIHRADYLAEASQSLSRSLSIEDTIDAMFDVAIPMLADRVVLALATDGVVTR
                     TEGYPRENGDAPQDLSEGLRAALHRALEHKHFELVREGDQAAAICPLLASGRARGALI
                     LLAPHERMDSSHVALAKEVVSRASIAMENARLYTAVQEADRRKNEFLAMLAHELRNPL
                     APIRNAVHIMQGGDVTQPVMTWARDVIGRQADHMARLIDDLLDVSRIVQGKVVVKPEE
                     LSLTALVERSVEASSPRVASRDQVLHVELPQQPVILIGDPVRLAQVLSNLINNASKFS
                     GPHSEITLTATHTDGEVKILVKDQGAGIDPAFLPHIFDLFAQADQSLDRSQGGLGIGL
                     TLVKHLVELHGGRVAARSAGIGRGTEIVVHLPAQVAPADEAPPPAPAPRKAAERSKLP
                     ARILVVDDLAASAETLMTLLEMEGFDVRVANEGMAALKIAEDFRPDVVLLDIGLPGMN
                     GFEVAHRLRTQPGSREALLIALTGYGEAESRLRSAQAGFDFHMVKPADVNLLLSMLAN
                     PQQARKARTV"
     gene            complement(2219..7204)
                     /locus_tag="VGA59_09640"
     CDS             complement(2219..7204)
                     /locus_tag="VGA59_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX9719980.1"
                     /translation="MGKEGKIKERAFVPNASGFWRDSAESINSLISDLVHPTSEVARV
                     IGAVAQGDLSKSMALEADGRPLEGEFLRTAMIINKMVQQLGTFSAEVTRVAREVGTEG
                     KLGGQADVQGVAGTWKDLTDSVNSMGSNLTAQVRNIAEVTKAVAAGDLSKKITVDVKG
                     EILELKNTINTMVDQLRSFASEVTRVAREVGTEGKLGGQADVQGVAGTWKDLTDSVNS
                     MGSNLTAQVRNIAEVTKAVAAGDLSKKITVDVKGEILELKNTINSMVDQLRSFASEVT
                     RVAREVGTEGKLGGQADVQGVAGTWKDLTDSVNSMGSNLTAQVRNIAEVTTAVAAGDL
                     SKKITVDVKGEILELKNTINTMVDQLSSFASEVTRVAREVGTEGKLGGQAEVQGIAGT
                     WKDLTDSVNSMGSNLTAQVRNIADVTTAVAAGDLSKKITVDVKGEILELKNTINTMVD
                     QLRSFASEVTRVAREVGTEGKLGGQADVQGVAGTWKDLTDNVNFMAGNLTSQVRNIAD
                     VTKAVAAGDLSKKITVDVKGEILELKNTINTMVDQLSSFASEVTRVAREVGTEGKLGG
                     QADVQGVAGTWKDLTDNVNFMAGNLTSQVRGIARVVTAVANGDLDRKLTVEAKGEIAA
                     LADTINSMTDTLATFADQVTTVAREVGVEGKLGGQAKVPGASGTWKGLTENVNQLAAN
                     LTTQVRAIAEVATAVTQGDLTRSITVEALGEVAALKDTINEMIRNLKDTTQVNTEQDW
                     LKTNLAKFSRMLQGQKDLVAVGHLILSELAPVVGAQQAEFYVLRYTHEQPKLRLMASY
                     ASEGHGAYGKEVDMGQGLVGQCAYDRKKILLTSSIPDSLRISSGLTGVAPLSVLVLPI
                     IFEGQVRGVVELASVERFNPTHQAFLDQLTESIGIVINTIEANMRTEDLLTQSQSLAQ
                     ELQSRQQELQQTNEELQEKARLLVHQNHEVERKNQEVEQARQALEDKAKQLALTSKYK
                     SEFLANMSHELRTPLNSLLILSDQLSKNPDGNLTAKQVEFARTIHSSGNDLLMLINDI
                     LDLSKIESGTVVVDVNELRLSDLQLYVERTFRHVAEAKSVDFLIHTGLNLPTSMVTDV
                     KRLQQILKNLLSNAFKFTHQGHVTLAIEEAFTGWTPDNEELNRAHQVIAFAVSDTGIG
                     ISGEKQQIIFEAFQQADGSTSRKYGGTGLGLAISRELSKLLGGEIRLSSAPGQGSTFT
                     LYLPLVYSGTRGPRRSLPSGESRGDATMPGLKRIPRLEPVVEDAVVLGDESAVGANEA
                     DDDRDAIQQGDLLLLIVENDLTFARFLLDAARAKGFKGLVTSMGAGALTLATQYKPSA
                     ITLDMYLPDMEGWRVLDRIKQDMSARHLPVCVISTDDSRDRAFRSGAMAFVEKPVQSK
                     EVLDTMLDQLQAYLARPRKSILVALKDVAARRDFIAQIEDEQIDVIVVKSAEKLMEAL
                     EDSRFDCVVLEDGFPGVDPADCRALLAAQAGLGPLPLLIYRGAGRDTGRFGWHSDDTL
                     TVQEAHNVARLFDSALLALHHNLSRLPEAKRSLINDIHESSKPLTGKRVLIVDDDMRN
                     IFALATVLEEHGMDIVWADNGRDAIARVATDPQIQVVLMDIMMPEMDGMATMKEIRKL
                     PAGKDLPMIAVTAKAMKGDREKCIEAGAWDYLSKPVNTHDLLAVMRAWLHQ"
BASE COUNT         1119 a         2455 c         2244 g         1399 t
ORIGIN      
        1 tgactttctg gcggagaggg cgggattcga acccgcggag ggctattaac cctcacacgc
       61 tttccaggcg tgcgacttaa accgctcatc cacctctccg cggagcccgc aattgtagca
      121 gcgggcctgc gccttgagct cgctcaaacc gtcctggcct tgcgcgcctg ctgcgggttg
      181 gccagcatcg acagcagcag gttaacgtcg gcgggcttga ccatgtggaa gtcgaacccg
      241 gcctgggccg agcgcaagcg gctctcggct tcgccgtagc cggtcagcgc gatcagcaaa
      301 gcctcgcgcg agccaggttg ggtgcgcagg cgatgcgcca cctcgaaacc attcatgccc
      361 ggcaggccga tgtccagcag cacaacgtcc ggccggaagt cctccgcgat cttcagggcg
      421 gccatgccct cgttcgcgac cctgacgtcg aagccttcca tctccagcag ggtcatcagc
      481 gtttcagccg aggctgcgag gtcgtccacc accaggatac gcgccggcag cttggagcgc
      541 tccgcggcct tgcgcggcgc cggcgccggc ggcggggcct cgtccgcggg cgcgacctgc
      601 gccggcaggt ggaccacgat ctccgtgccc cgcccgatac ccgcgctcct ggccgcaacc
      661 cggccaccat gcagctcgac cagatgcttg accagggtca ggccgatgcc cagtccgcct
      721 tgcgagcggt ccagggactg gtcggcctgg gcgaacaggt cgaagatgtg cggcaggaac
      781 gccggatcga tgcccgcgcc ctggtccttg accaggatct tcacctcgcc atcggtatgc
      841 gttgccgtca gggtgatctc gctgtggggt ccggaaaact tggaggcgtt gttgatgagg
      901 ttggacagaa cctgcgccag ccgcaccggg tcgccgatca gaatgacggg ctgttggggc
      961 agttccacgt gcagcacctg gtcgcggctg gccactcgcg gggaactcgc ctccacggat
     1021 cgctcgacca gggccgtcag gctgagctcc tcgggcttga ccaccacctt gccctggacg
     1081 atgcgcgaaa cgtcgagcag gtcatcgatg agccgcgcca tgtgatcggc ctgccggccg
     1141 atcacatcgc gggcccaggt catgacgggc tgcgtgacgt ctccgccctg catgatgtgc
     1201 acggcattgc ggatcggcgc cagcgggttg cgcagctcgt gcgccagcat tgccaggaat
     1261 tcgttcttgc gccggtccgc ctcctgtacg gccgtgtaga gccgcgcgtt ctccatggcg
     1321 atcgatgcgc ggctcaccac ctccttggcc agcgcaacgt gcgatgaatc catgcgctcg
     1381 tgcggcgcca gcaggatgag cgccccgcgc gcccggccgc tcgccagcag cgggcagatc
     1441 gcggcggcct ggtcgccctc gcgaaccagt tcgaagtgct tgtgctccag cgcccgatgg
     1501 agggcggctc gcaagccttc ggacaagtcc tgcggcgcat ccccgttctc ccggggatag
     1561 ccctcggtac gcgtgacgac tccgtccgtg gcgagcgcca gcacgacccg gtcggccagc
     1621 atgggaatgg ccacatcgaa catcgcgtcg atggtgtcct cgatgctcag cgaccgcgac
     1681 agcgactggc tggcttcggc cagatagtcg gcgcgatgga tggcgctttc cgcggcgtcg
     1741 cgcgccgcct ccgatcgggc cagcgcctcc cgctgcgccg cctgcacctg cagctgctgg
     1801 ttcatgcggt acagctcgac gaacaccctc accttcgaac gcagcacctc gggcacaacg
     1861 ggcgaaggga tgtagtccac cgcgccgagc gcatagccgc gcttggcctg ccgctcatcc
     1921 acgtaagcgg tgatgaaaac gatgggggtc tgggccgact tcttgtactg ccgtatcagg
     1981 ctggcggttt ccaggccgtc gatgtccggc atgttgacgt ccagcaggat gacggcgaac
     2041 tccttttcca gaatgaaacg cagcgcctcc tgccccgagc gggcactgac gatgttctgg
     2101 ccgagctctt ccaggatgct gcgaaagacg atgtgcttct cgggcagatc gtccaccacc
     2161 aggatgttgg ccttctccct ttcggcaatt tgcggcagcg tcgtcggcac cggattcctc
     2221 attgatgcag ccatgcacgc atcaccgcga gcaggtcatg cgtgttgacc ggtttcgaca
     2281 ggtagtccca ggcaccggcc tctatgcatt tctcgcggtc gcctttcatt gccttggcgg
     2341 ttaccgcgat cattggcagg tccttgccgg ccgggagctt gcggatttcc ttcatcgtcg
     2401 ccatcccgtc catctcgggc atcatgatgt ccatcaatac cacctggatc tggggatcgg
     2461 ttgcgacccg ggcgatcgca tctcggccat tatcggccca gacgatatcc atcccatgct
     2521 cttccagaac agtggcgagc gcgaaaatgt tgcgcatgtc gtcgtcgacg atcagcacgc
     2581 gtttgcccgt caggggcttg ctggactcat ggatgtcatt gatgagcgac cgcttggcct
     2641 ccgggagccg cgacaggttg tggtgcagcg ccaacaacgc actgtcgaac agcctggcca
     2701 cgttatgggc ctcctgcacc gtgagggtgt cgtcggagtg ccagccgaac cttcccgtgt
     2761 ccctgccggc gccgcggtag atcagcagcg gcaacgggcc gagcccggcc tgggccgcca
     2821 gcagcgcgcg gcagtccgcg ggatcgacac ccgggaaacc gtcttccagc accacgcagt
     2881 cgaagcggct gtcttccagc gcttccatca gcttttcggc gctcttcacg acgatcacgt
     2941 cgatctgctc gtcctcgatc tgggcgatga agtcgcgccg cgccgcgacg tccttgagcg
     3001 cgacgagaat gctcttgcgc ggccgcgcca ggtaggcctg aagctggtcg agcatggtgt
     3061 cgagcacctc cttggactgg accggcttct cgacgaaagc catcgcgccc gaccgaaatg
     3121 cccggtcccg cgaatcgtcg gtggagatca cgcacaccgg gagatggcgc gcgctcatgt
     3181 cctgcttgat gcggtcgagc acgcgccagc cttccatgtc cggcaggtac atgtccagag
     3241 taatggccga gggcttgtac tgcgtggcca gcgtgagggc gcccgcgccc atcgaggtca
     3301 cgagcccctt gaagcccttc gcgcgcgccg catcgagcag gaagcgggca aaggtcaggt
     3361 cgttctccac gatcagaaga agcaggtcgc cctgctggat ggcatcgcga tcgtcatccg
     3421 cctcattcgc gccgacggcg ctttcgtcgc cgagcaccac ggcgtcctcc accaccggtt
     3481 cgaggcgcgg gatccgcttg aggcccggca tcgtcgcgtc gccgcggctc tcgccggatg
     3541 gcagcgaacg gcgggggccg cgcgtgccgg aatacaccag cggcaggtac agggtgaagg
     3601 tgctgccctg ccccggtgcg ctggacaacc ggatctcgcc gcctagcagc ttggacagct
     3661 cgcggctgat ggccaggccc aggccggtgc caccgtactt gcggctggtc gagccgtcgg
     3721 cctgctggaa ggcctcgaaa atgatctgct gcttttcgcc cgagatgccg atgccggtgt
     3781 ccgatacggc gaaggcgatc acctggtggg cacggttgag ttcctcgttg tcgggggtcc
     3841 agccggtgaa cgcctcttcg atggcgagag tcacgtgccc ctggtgggtg aacttgaagg
     3901 cgttcgacag caggttcttc aggatctgct gcaggcgctt cacgtccgtg accatcgaag
     3961 tcggcaggtt caggccggtg tggatcagga agtccacgct cttggcctcg gccacgtggc
     4021 ggaaggtgcg ctccacataa agctgcaggt ccgacaggcg cagctcgttg acgtccacca
     4081 ccacagtgcc cgactcgatc ttggacaggt ccaggatgtc gttgatgagc atcagcaggt
     4141 cgttgccgga agagtggatg gtcctggcga actccacctg cttggccgtc aggttgccgt
     4201 ccgggttctt ggagagctgg tcggacagga tcagcagcga gttcagcggc gtgcgcaact
     4261 cgtgcgacat gttggccagg aactcagact tgtacttgga cgtcagcgcc agctgcttgg
     4321 ccttgtcttc cagcgcctgg cgggcctgct ccacctcctg gttcttgcgc tccacctcgt
     4381 ggttctggtg caccagcagg cgggcttttt cctgcagctc ctcgttggtc tgctgcagct
     4441 cctgttgccg gctttgcagt tcctgcgcca gcgactgcga ctgggtcagc aggtcttcgg
     4501 tccgcatgtt ggcttcgatc gtgttgatca cgatgccgat cgactcggtc agctggtcca
     4561 ggaaggcctg gtgcgtcggg ttgaaccgct ccaccgaagc gagctcgacc acaccgcgga
     4621 cctgcccctc gaaaatgatg ggaagcacca gcacgctcag tggtgccacc ccagtcaggc
     4681 ccgaagagat cctcaggctg tccggaatcg acgaggtcag caggatcttc ttgcgatcgt
     4741 aggcgcactg gccgaccagg ccctggccca tgtccacttc cttgccgtaa gcgccgtggc
     4801 cctccgacgc gtagctggcc atcagccgca gcttgggctg ctcgtgcgtg tagcgcaaca
     4861 cgtagaactc ggcctgctgg gctcccacca ccggagccag ctcggaaagg atcaggtgcc
     4921 cgaccgccac caggtccttc tggccctgca gcatccgcga gaacttggcc aggttggtct
     4981 tgagccaatc ctgctcggta ttcacctgcg tcgtgtcctt caggttgcgg atcatctcgt
     5041 tgatggtgtc cttcagcgcc gcgacttcgc ccagcgcctc caccgtgatc gaacgggtca
     5101 agtcgccctg ggtcacggcg gtggccacct cggcaatggc ccgcacctgg gtcgtcaggt
     5161 tggccgcgag ctggttcacg ttctccgtca ggcccttcca ggtgcccgaa gcgcccggca
     5221 ccttggcctg gccgcccagc ttgccttcga cccccacttc acgcgccacg gtcgtcacct
     5281 ggtccgcgaa ggtcgcgagc gtgtcggtca tcgagttgat ggtatcggcc agggcggcga
     5341 tttcgccctt ggcttccacg gtcagcttcc ggtcgaggtc gccgttcgcc accgcggtca
     5401 ccacccgggc gataccgcgc acctgggaag tgaggttgcc ggccatgaag ttcacgttgt
     5461 cggtcaggtc tttccaggtg ccggccacgc cctgcacgtc ggcctgcccg cccagcttgc
     5521 cttcggtacc cacttcacgg gccacccgcg tcacttccga tgcaaacgag gaaagctggt
     5581 ccaccatggt gttgatggtg ttcttcagct ccaggatttc gcccttcacg tccaccgtga
     5641 tcttcttgga caggtcacca gcggccacgg ccttggtcac gtcggcgatg ttgcgcacct
     5701 gcgaggtcag gttgccggcc atgaagttca cgttgtcggt caagtctttc cacgtgccgg
     5761 ccacgccctg cacgtcggcc tggccgccca gcttgccttc ggtacccact tcccgggcca
     5821 cccgcgtcac ttccgaggcg aacgatcgca gctggtccac catggtgttg atggtgttct
     5881 tcagctccag tatttcgccc ttcacgtcca ccgtgatctt cttggacagg tcaccggcgg
     5941 ccacggcggt cgtcacgtca gcgatgttgc gcacctgggc cgtgaggttc gagcccatgg
     6001 aattcaccga gtcggtcaag tccttccagg tgccggcaat gccctgcacc tcggcttggc
     6061 cgcccagctt gccttcggtc cccacttccc gggccacccg cgtcacttcc gaagcgaacg
     6121 aggagagctg gtccaccatg gtgttgatgg tgttcttcag ctccagaatc tcgcctttca
     6181 cgtccaccgt gatcttcttg gacagatcgc cggcggccac ggcggtcgtc acttccgcaa
     6241 tgtttcgcac ctgggccgta aggttcgagc ccatggaatt caccgagtcg gtcaagtcct
     6301 tccacgtgcc ggccacgcct tgcacgtcgg cctgcccgcc cagcttgcct tcggtaccca
     6361 cttcccgcgc cacccgcgtc acttccgagg cgaacgatcg cagctggtcc accatgctgt
     6421 tgatcgtgtt cttcaactcc aggatttcgc ccttcacgtc caccgtgatc ttcttggaca
     6481 ggtcaccggc cgccaccgcc ttggtcacct ccgcgatgtt gcgcacctgg gccgtgaggt
     6541 tcgagcccat ggagttcacg gaatcggtca agtccttcca ggtgccggcc acgccctgca
     6601 cgtcggcctg gccacccagc ttgccttcgg tgcccacttc gcgcgccacc cgcgtcactt
     6661 ccgacgcgaa ggagcgcaac tggtccacca tggtgttgat ggtgttcttc agctccagaa
     6721 tctcgccctt cacgtccacc gtgatcttct tggacaagtc accggcggcc acggccttcg
     6781 tgacctcggc gatgttgcgg acctgagccg tgaggttgga gcccatggag ttcacggagt
     6841 cggtcaagtc cttccaggtg ccggccacac cctgtacgtc ggcctgcccg cccagcttgc
     6901 cttcggtacc cacttcccgc gccacacgcg tcacttcggc ggagaaggtg cccagctgct
     6961 gaaccatctt gttgatgatc atggcggtgc gcaggaactc gccttccagc ggccggccat
     7021 cggcctcgag cgccatgctc ttggacagat cgccctgggc gacggcgccg atcacccgcg
     7081 ccacttcgga cgtcgggtgc accaggtcgg agatcagcga gttgatggac tcggctgaat
     7141 cgcgccagaa gccgctcgcg ttgggcacga aggcgcgctc tttgatcttg ccctctttac
     7201 ccaccacccg cgacagc
//
LOCUS       DASWIY010000261         2684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1273336, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000261 DASWIY010000000
VERSION     DASWIY010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273336"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VGA59_09645"
     CDS             complement(<1..771)
                     /locus_tag="VGA59_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9719981.1"
                     /translation="MNARLDLDRANSDVVLIVDDVPDNLAVLHDALDESGYTVLVATG
                     GEAALQRAAQALPDIVLLDAMMPGMDGFEVARRLKASPLTAHIPIIFMTGLTETEHLV
                     AALEAGGVDYVTKPIKPKEVLARMGVHMQGARQARQTRNALDAFGYASITVRASDGKL
                     MWQTPLARELLLAYYGTSAPESPRPVVEWLRRHLKEAQQQIEPPRLTAELGARRLTFR
                     LHQQIGNDDEGGDWLIVMREVSDTAVIEAMSLSFKLTAR"
     gene            complement(768..>2684)
                     /locus_tag="VGA59_09650"
     CDS             complement(768..>2684)
                     /locus_tag="VGA59_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9719982.1"
                     /translation="RCVICEREYEGPDMAHCPAYQGQICSLCCTLDARCGDLCKPQAS
                     LSAQWSGALRWLLPRRVWPYLDTGLSHFLLLMLIVVPLLAALFGLLYHQEVRAVAQLL
                     QDADALGATLRSGFLKAYMALLVIAGIVAWWLVLAHKSRQVAQEESNRQTHLLMREIE
                     SHQQTDAALKSARLLAEQANQAKSRYISAISHELRTPLNSILGYAQLMGEDASVPAHR
                     KQAVNVIKRGGEHLLSLIEGTLDIARIESGKLTLDIKPMQFADCVHEMAGMFEPQATA
                     KGLAFRFDAEGSLPEVVRADEKRVRQILINLLGNAIKFTAQGQVIFRVRYAREMAHIE
                     IEDTGPGLGRAELAQVFEPFARGNSASHTAPGAGLGLTIAKMLTDLMGGELTVASTPG
                     VGSVFRIKLFLPEARHPVAVRRATTHAPRRGYEGVRRKILVVDNEEADRELLVQLLEP
                     LGFELRTAASGHDALDLLAAGLQPDAILMDLAMPGIDGWETIRRLREMGHVNVKVAVV
                     SANAFDKGLDNGAGIPPEDFILKPLRHGELLDWLQRQLELRWLQEPRVAAPTQRQSAG
                     VLRRPAPQSLQALRQAVQLGYFRGIMNQLDEIASAQPECGEFAAAMRELASQFQFEAM
                     SRELAGAAPALEDE"
BASE COUNT          410 a          928 c          874 g          472 t
ORIGIN      
        1 gcgcgccgtt agcttgaacg acaggctcat ggcttcgatc accgccgtgt ccgagacctc
       61 gcgcatcacg atcagccagt cgccgccttc gtcgtcattg ccgatctgct ggtgcaaccg
      121 gaaagtgagg cggcgagcgc ccaattccgc cgtcaaccgc gggggctcga tctgctgctg
      181 cgcctctttt aggtgccggc gcagccactc gaccaccggc cgcggcgact ccggtgcgct
      241 ggtgccgtaa tacgccagca gcagctcccg cgccaggggc gtttgccaca tcagtttgcc
      301 gtcgctggcc cgcaccgtga tgctggcgta gccgaatgcg tcaagggcgt tgcgcgtctg
      361 ccgggcctga cgcgcgccct gcatgtgcac acccatgcgc gccagcactt ccttgggctt
      421 gatgggcttg gtgacgtaat ccaccccgcc ggcttccagc gccgcgacca ggtgctcggt
      481 ttcggtgagg ccggtcatga agatgatggg aatatgcgct gtcaggggcg aggccttcag
      541 ccgcctggcg acctcgaaac cgtccatgcc cggcatcatc gcgtccagca gcacgatgtc
      601 gggcaaagcc tgggcggcgc gctgcaaggc cgcttcgccg ccggtcgcca ccagcacggt
      661 gtagcccgat tcgtccagcg cgtcgtgcag caccgccaga ttgtcgggca catcgtccac
      721 gatcagcacg acgtcgctgt tggcgcggtc caggtccagt cttgcgttca ttcgtcctcc
      781 agcgcaggag ccgcgccggc cagctcacgg ctcatggcct cgaactggaa ctggcttgcg
      841 agctcgcgca tggcggcggc gaactccccg cattcgggct gggccgacgc gatctcgtcg
      901 agctggttca tgatgccgcg gaagtagccc agctggaccg cctgccgcaa tgcctgtagc
      961 gattgcggtg ccggccgccg taagacaccg gccgattggc gttgcgttgg cgcggcgacg
     1021 cggggctcct gcagccagcg cagctccagc tgccgctgca gccaatcgag cagctcgccg
     1081 tggcgcaacg gcttgaggat gaaatcttcc ggcggaatgc ccgcgccgtt gtccagcccc
     1141 ttgtcgaagg cattggccga taccacggcc accttgacgt tcacgtgccc catctcgcgc
     1201 agccttctga tggtttccca gccgtcgatg ccgggcatgg ccaggtccat caggatcgca
     1261 tccggctgca ggcccgcggc tagcaggtcg agcgcatcat gaccactggc ggcggtgcgc
     1321 agctcgaacc ccagcggttc cagcaattgc accagcagct cgcggtcggc ctcttcgttg
     1381 tccaccacca gaatcttgcg gcgcacgcct tcatagcccc ggcgcggcgc gtgggtcgtg
     1441 gcccggcgta ccgcaaccgg atggcgtgct tccggcagga atagcttgat gcggaacacc
     1501 gaacccacgc cgggcgtgct tgcgaccgtg agctcaccac ccatcaggtc ggtgagcatt
     1561 ttggcgatgg tcaggcccag ccccgcgccg ggcgctgtat ggctggcgct gttgccacgg
     1621 gcaaacggct cgaagacttg cgccagttcg gctcggccga ggccggggcc ggtgtcctcg
     1681 atctcgatgt gcgccatttc gcgcgcgtat cgcacgcgga agatcacctg cccctgcgca
     1741 gtgaacttga tcgcattgcc cagcaggttg atcaggatct gccgcacccg cttctcgtcg
     1801 gcgcgcacca cttccggcag ggagccctcg gcatcgaacc ggaacgccag ccccttggcg
     1861 gtggcctgcg gctcgaacat gccggccatc tcgtgcacgc aatcggcgaa ctgcatgggc
     1921 ttgatgtcga gcgtgagctt gcccgactcg atgcgcgcga tgtccagggt gccctcgatc
     1981 agcgacagca ggtgctcgcc gccgcgtttg atgacgttga cggcctgctt gcggtgcgcc
     2041 ggcacgctcg catcctcacc catcagctgc gcatagccga gtatgctgtt gagcggtgtg
     2101 cgcagctcgt ggctgatcgc gctgatgtag cggcttttgg cctggttggc ctgttccgcc
     2161 aggagcctcg cgcttttgag cgcggcgtcg gtctgctggt gcgattcgat ctcgcgcatc
     2221 agcaggtggg tctgccggtt cgattcctcc tgtgccacct ggcggctctt gtgcgccagc
     2281 accagccacc acgcgacgat gccggctatc accagcagtg ccatgtaggc cttgaggaat
     2341 ccggatcgca gcgtggcccc caaggcatcc gcgtcctgca gcagttgcgc cacggcccgc
     2401 acctcctggt gatagagcag gccgaacagg gcggccagca gcggcaccac gatcagcatc
     2461 agcagcagga aatgcgacag gcccgtgtcc aggtagggcc agacccgccg cggcagcagc
     2521 cagcgaaggg cccccgacca ttgggccgac aggctcgcct gcggcttgca caggtcgccg
     2581 cagcgcgcgt ccagcgtgca gcacagcgaa cagatctggc cctggtaggc cgggcaatgc
     2641 gccatgtcgg ggccttcgta ctcgcgctcg cagatcacgc agcg
//
LOCUS       DASWIY010000262         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_548723, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000262 DASWIY010000000
VERSION     DASWIY010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548723"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VGA59_09655"
     CDS             complement(<1..202)
                     /locus_tag="VGA59_09655"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX9719983.1"
                     /translation="MSTGHKGNMRGVAVAKPLPKVATGIAGFDDLSRGGLPRGRTTLL
                     KGGPGSGKTVFALQCLVNAARQR"
     gene            complement(293..433)
                     /locus_tag="VGA59_09660"
     CDS             complement(293..433)
                     /locus_tag="VGA59_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEX9719984.1"
                     /translation="MNITLSLAPLISLVAGILILAVPRLLNYIVAAYLIIVGIIGLFR
                     LG"
     gene            624..773
                     /locus_tag="VGA59_09665"
     CDS             624..773
                     /locus_tag="VGA59_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX9719985.1"
                     /translation="MLFTIAVVLVVLWFLGLVTANTLGGLIHALLLIAVIILLVRVIS
                     GRKAL"
     gene            770..985
                     /locus_tag="VGA59_09670"
     CDS             770..985
                     /locus_tag="VGA59_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 family protein"
                     /protein_id="HEX9719986.1"
                     /translation="MNGVFKAAALALLAGGVVLLVFGVNAMNSPGSDISRFFTGAPTD
                     RATWMLVGGIAMAVAGLAGLLLALRKS"
     gene            complement(993..>1606)
                     /locus_tag="VGA59_09675"
     CDS             complement(993..>1606)
                     /locus_tag="VGA59_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719987.1"
                     /translation="VRDGAVESRMVFRFKDGSLMDETVVFTQERVFLMQRYRLLQQGP
                     VFDEDSEISLERASGKYRVKTTAHKRGAQKVLEGTLDMPADTYNGMVINIAKNLPQGA
                     RETVHIVAFTPAARLVELEMTQAGEHKVLVGELAKSAAHYVFKPRPGAWLTLLATLLG
                     RMPPDSHTWIVTDEVPAFVRFEGPLHPAGPAWRIELTSPRWPE"
BASE COUNT          243 a          531 c          544 g          288 t
ORIGIN      
        1 gccgctggcg cgcggcgttg accaggcatt gcagcgcgaa cacggtcttg cccgagcccg
       61 ggccgccctt gaggagcgtg gtgcgcccgc gcggcagccc gccccggctc aggtcgtcga
      121 aacccgcgat gccggtggcg accttgggca aaggcttggc aactgccacc cctcgcatat
      181 tacccttgtg tccggtgctc atgttcgata ccggacgtac ggcaagccct gcggcacggg
      241 cccggggcgg tatgcgcgat gacacacgta gccacccgcg gagggtggtg cctcagccga
      301 ggcggaagag gccgatgatg ccgacgatga tgaggtaggc cgcaacgatg tagttcagca
      361 ggcgcggcac cgccagaatg agaattccgg ccaccagcga tatcaggggc gccaggctca
      421 gggtgatgtt catgaggctt cctttgtagc agtgcgaagg gtgatcggcg ttcgtgcgcg
      481 atgctcaccg atgcgcgggc tgccgtatgt tcgccaacgc accggagaat tcgccgggcc
      541 cggcgcccgt atgtcggcca gcgcacagaa cagcgccggg gcaaagggca ccattgccgc
      601 gtcacgtcaa aggaatgcga ggcatgcttt tcaccatcgc cgtcgtgctg gtcgttttgt
      661 ggttcctggg gctggtcacc gccaatacgc tgggcggcct catccacgcg ctgctgctga
      721 tcgcggtcat cattcttctg gtgcgcgtca tttccgggcg caaggcgctg tgaacggcgt
      781 gttcaaggca gccgcgctcg ccctgctcgc cgggggcgtc gtgctgctcg tcttcggcgt
      841 gaacgccatg aattcgcccg gctccgacat ttcccggttt ttcaccggcg ccccgactga
      901 ccgggccacc tggatgctgg tcgggggcat cgcgatggcc gtcgcgggcc tcgccggatt
      961 gctgctggcc ctccggaaaa gctgaacgcc ggctattcgg gccagcgcgg gctcgtcagc
     1021 tcgatgcgcc acgccggtcc cgccgggtgc agcgggccct cgaagcgcac gaaggccggc
     1081 acttcgtcgg tgacgatcca ggtgtgggag tccggcggca tgcgcccgag cagggtggcg
     1141 aggagcgtga gccaggcgcc cggcctgggc ttgaacacgt aatgggccgc gctcttcgcc
     1201 agctcgccga ccagcacctt gtgctcgccg gcctgtgtca tctccagctc gacgagccgc
     1261 gccgcgggcg tgaaggcgac gatgtgtacg gtctcgcgcg ccccctgggg aagatttttc
     1321 gcgatgttga tgaccatgcc gttgtaggtg tcggcaggca tgtcgagcgt gccctcgagc
     1381 accttctgcg ccccgcgttt gtgcgccgtg gtcttcacgc gatacttccc ggaggcgcgc
     1441 tccagcgaga tctcgctgtc ttcgtcgaat acgggacctt gttgcagcag gcgatagcgc
     1501 tgcatcagga acacgcgctc ctgggtgaac accaccgtct cgtccatcaa cgagccgtcc
     1561 ttgaagcgga acaccatgcg gctctccacc gccccatcgc gcaccg
//
LOCUS       DASWIY010000263         3196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1663704, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000263 DASWIY010000000
VERSION     DASWIY010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663704"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /gene="rlmJ"
                     /locus_tag="VGA59_09680"
     CDS             <1..891
                     /gene="rlmJ"
                     /locus_tag="VGA59_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEX9719988.1"
                     /translation="GNHADVLKHTMLIAMLAHLTQKDTALTVIDTHAGAGLYRLDGDY
                     AGTSGEAADGIVKLFAALAPADPARAVIKDIAPVLQDYLQMVAAFNGLGQLKVYPGSP
                     FIAQRLLRDEARDKLKLFELHPTDIKALAGNIAQLKAGRQVAVAREDGFEGLKAFLPP
                     PSRRALVLIDPSYEIKTDYQKVGACIQDSLKRFATGTYAVWYPIIPRPEAHELPRRLK
                     TLATQAGKPWLHATLAIGQSEERNVPGEPERGPGLTASGMFVINPPHTLKPALQAALP
                     QLLQLLGRGKGQGQTLESAG"
     gene            1122..2141
                     /gene="paaA"
                     /locus_tag="VGA59_09685"
     CDS             1122..2141
                     /gene="paaA"
                     /locus_tag="VGA59_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX9719989.1"
                     /translation="MYTQALDLPDTDQRDLKAVPPPAEAGESALQAHFDGKIDADRKI
                     EPQDWMPEAYRKTLVRQMSQHAHSEIVGMLPEGNWISRAPSLKRKAILLAKVQDEGGH
                     GLYLYSAAETLGVSRDALVDALHSGKAKYSSIFNYPTLTWADVGTIGWLVDGAAIMNQ
                     VPICRCSYGPYARAMIRICKEESFHQRQGFDSLMAQMKGTPAQREMVQDAVKRWWWPV
                     LAMFGPPDTNSPNSALGMRWGIKRVSNDDLRQKFVDATVPQAKILGVTLPDPGLKWNE
                     ERRHYDYGAIDWDEFWAVVNGNGPCNKERLATRVKAHADGQWVRDAALAHARKKQARE
                     LKEAA"
     gene            2324..2608
                     /gene="paaB"
                     /locus_tag="VGA59_09690"
     CDS             2324..2608
                     /gene="paaB"
                     /locus_tag="VGA59_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEX9719990.1"
                     /translation="MRREWPLWEVFVRSKQGLEHKHCGSLHAADAQQALQMARDVYTR
                     RQEGVSIWVVPSALITASEPDDKAEFFEPAADKIYRHPSFYEIPDEVGHM"
     gene            2608..>3196
                     /gene="paaC"
                     /locus_tag="VGA59_09695"
     CDS             2608..>3196
                     /gene="paaC"
                     /locus_tag="VGA59_09695"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX9719991.1"
                     /translation="MGEAMQQAPVTAPIDYLLHLADNALVLGQRNAEWCGHGPALEED
                     IAMANMSLDLIGQARLLYQHAAALAGAGATEDSLAYFRDAGQFRNYTLLELPHHGPLA
                     GTAAANRDYATTIARNFLYSALMRLLWGSLQESKDAQLAAIAAKSLKEVRYHLRHARD
                     WLVRLGDGTGESHARMQEALDHLMAYTQEFWTVTSA"
BASE COUNT          604 a         1055 c         1063 g          474 t
ORIGIN      
        1 ggcaatcacg ccgatgtgct caagcacacg atgctgatcg ccatgctggc gcacctgacg
       61 cagaaggaca cggccctcac agtgatcgac acccacgcgg gcgccggctt gtaccggctc
      121 gatggcgact acgcgggcac ctcgggcgag gcggcggacg gcatcgtcaa gctgtttgcc
      181 gcgctggcgc cggccgatcc ggcccgggcc gtcatcaagg acatcgcgcc cgtgctgcag
      241 gattacctgc aaatggtggc cgccttcaac gggttgggcc aattgaaggt ctatccgggg
      301 tcgcccttca tcgcccagcg actgttgcgc gatgaggcgc gcgacaaact caagctgttc
      361 gagctgcacc ccaccgacat caaggccctg gccggcaaca tcgcccagct caaggcgggg
      421 cgccaggtgg ccgttgcgcg cgaggacgga ttcgaaggac tgaaagcctt cctgcctcca
      481 ccctcgcgcc gcgcgctggt gctgatcgac ccgagttacg agatcaagac cgattaccag
      541 aaggtcggcg cctgtatcca ggacagcctc aagcggttcg ccaccggtac ttatgccgtc
      601 tggtacccca tcattccgcg gcccgaagcg catgagctgc cgcggcgcct gaaaacgctg
      661 gccacgcagg ccggcaagcc ctggctgcac gccacgctgg ccatcggcca gtcggaagag
      721 cgcaatgtgc cgggcgagcc cgaacgcggg ccgggcctga cggccagcgg gatgttcgtc
      781 atcaatccgc cccacacgct caagcccgcg ctccaggcag cgctgcctca gttgctgcag
      841 ctgctcggcc gtggaaaggg tcaggggcag acgctggaat ccgcgggttg aaagtgagtt
      901 tgtcattgcg ggcctgaccc gcaatccatg aacccctaat acctggatcc cgggtcaagg
      961 ccgggatgac aggttccggg tcaaggccgg gatgacaggt tccgggtcaa gcgcgggatg
     1021 acaggttccg ggtcgagccc tggatgacaa gtctcaaagc tgggctgtag ggcttttttc
     1081 attaaccaac caatcggtcg gttaattact atactgcctg gatgtacacg caagccctgg
     1141 atcttcccga cacggaccag cgagacctca aggccgtgcc gccgccggcc gaggctggcg
     1201 agtcggctct gcaggctcac ttcgacggca agatcgacgc cgatcgcaag atcgagccgc
     1261 aggactggat gccggaggcg taccgcaaga ccctggtgcg ccagatgagc cagcacgccc
     1321 acagtgaaat cgtcggcatg ctgcccgaag ggaactggat ctcccgcgcc ccttcgctca
     1381 agcgcaaggc catcctgctg gccaaggtgc aggacgaagg cggccacggc ctgtacctct
     1441 actccgcggc cgaaacgcta ggcgtcagcc gcgatgccct ggtcgacgcg ctgcacagcg
     1501 gcaaggccaa gtacagctcg atcttcaact acccgaccct gacctgggcc gacgtcggca
     1561 ccatcggctg gctggtggac ggcgccgcga tcatgaacca ggtgccgatc tgccgctgct
     1621 cctacgggcc ctatgcgcgc gcgatgatcc gcatctgcaa ggaagaaagc ttccaccagc
     1681 gccagggctt cgacagcctg atggcgcaaa tgaagggaac gccggcgcag cgcgagatgg
     1741 tgcaggacgc cgtgaagcgc tggtggtggc cggtgctggc gatgttcggc ccgccggaca
     1801 ccaacagccc gaacagcgcg ctcggcatgc gctggggcat caagcgcgtc agcaacgacg
     1861 acctgcgcca gaagttcgtg gatgcgaccg tgccgcaggc gaagatcctc ggcgtgacgc
     1921 tgcccgaccc cggcctgaaa tggaacgagg agcgccggca ctacgactac ggggcgatcg
     1981 actgggacga attctgggcc gtggtcaacg gcaacggccc gtgcaacaag gaacgcctgg
     2041 cgacgcgcgt gaaggcgcac gccgacggcc aatgggtgcg cgacgcggcc ctggcgcatg
     2101 cgcgcaagaa gcaggcgcgc gaattgaagg aggccgcatg acacagagcc ctcaccccag
     2161 ccctctcccg caagcgggag agggagccgt cgagccaacg gggatgtccg aggacgttca
     2221 ccccgcacgc ctgcaagcgg gagaggcggt tggagttcct tcttcctccc tctccaccgc
     2281 tcgcggggga gagcttgcga gggggccggg cgggggtgag ggggtgcggc gggaatggcc
     2341 cctctgggaa gtgttcgttc gctccaagca gggcctggaa cacaagcact gcggcagcct
     2401 gcacgcggcc gacgcgcaac aggcgcttca gatggcgcgc gatgtgtaca cccgccgcca
     2461 ggaaggcgtg agcatctggg tcgttccctc ggcgttgatc accgccagcg agcccgatga
     2521 caaggcggag ttcttcgagc ccgccgccga caagatctac cgccacccga gcttttacga
     2581 aatccccgac gaagtcggcc acatgtgatg ggcgaggcga tgcagcaggc accggtgacg
     2641 gcgccgatcg actatctttt gcacctcgcc gacaacgccc tggtgctcgg ccagcgcaac
     2701 gccgaatggt gcggccatgg gccggcgctg gaagaggaca tcgcgatggc gaatatgagc
     2761 ctggacctga tcgggcaggc acggctgctg taccagcacg cggcggcgct cgcgggcgcg
     2821 ggcgcgaccg aggacagcct ggcctatttc cgcgacgcgg gccagttccg caactacacg
     2881 ttgctggaat tgccgcatca cgggccgctg gccggcaccg ccgcggcaaa ccgcgactac
     2941 gccaccacca tcgcacgcaa ttttctctac agcgcattga tgcggctgct ctggggctcg
     3001 ctgcaggaat ccaaagacgc gcaattggcg gcgatcgccg ccaagtcgct caaggaagtc
     3061 cgctaccacc tgcggcatgc gcgcgactgg ctggtgcggc tgggcgacgg caccggcgaa
     3121 tcgcacgcgc gcatgcagga ggcgctcgat catttgatgg cttacacgca ggagttctgg
     3181 accgtcacgt cggccg
//
LOCUS       DASWIY010000264        24313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3142533, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000264 DASWIY010000000
VERSION     DASWIY010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 24313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24313
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3142533"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /gene="bamC"
                     /locus_tag="VGA59_09700"
     CDS             complement(<1..874)
                     /gene="bamC"
                     /locus_tag="VGA59_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEX9719992.1"
                     /translation="MKQFARFGVVAMCAALSACTILEGEKIDYKSAGKGPSLEVPPDL
                     TQLSRDSRYTVPGGPVTASSFQVGQAAPGVPTAASTLGDVRIERAGTQRWLVVNRSAD
                     ELWNPVREFWQESGFLLALDQASLGIMETDWAENRAKLPQDFIRNALGKVFDSLYSTA
                     ERDRFRTRLERNASGGTEIYVSHRGMIEVYSNTQKDQTVWQPRPSDPELEAEFLRRLM
                     VKLGVPQEQSKALVASGAAKTTSRVATVGNTPVVQIDEGFDRAWRRVGLTLDRTGFTV
                     EDRDRAQGMYFVRYV"
     gene            complement(906..1790)
                     /gene="dapA"
                     /locus_tag="VGA59_09705"
     CDS             complement(906..1790)
                     /gene="dapA"
                     /locus_tag="VGA59_09705"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX9719993.1"
                     /translation="MTPITGSIVALATPMHDDGSVDYPTLRKLIDWHIAEGTDCIGVV
                     GTTGESPTVNVQEHHEIIRVAVEQANKRVPIMAGCGANSTAEAVELAKFARKIGADCQ
                     LQVVPYYNKPTQEGQYLHFKAIAEAVDLPIILYNVPGRTVADLLHDTVLRLAQVPGIV
                     GIKEATGNIERAQWLVREVPKGFSVYSGDDPTAVALMLCGGQGNVSVTANVAPRLMHE
                     LCVAAVAGDARRAMEIQRKLMPVHKNLFVEANPIPVKWAMARMGLCGGALRLPMTTLA
                     RANEAAVEGALRAAGLLA"
     gene            complement(1851..2426)
                     /locus_tag="VGA59_09710"
     CDS             complement(1851..2426)
                     /locus_tag="VGA59_09710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9719994.1"
                     /translation="MHGTESPSAWVQRWTHLVPAGGAVLDVACGFGRHMRWFSQRGHP
                     VTGVDRAPEAIDAVSSLGEAVLADIENGPWPFPSRTFGGVVVTNYLWRPLWPTLLQSV
                     APGGALIYETFTAGHESVGKPSRPDFLLQPGELLRACSELQVVAFEDGFLDEPARFVQ
                     RIAAVRPAPGEPRPRYRLQHPPGIAAPEQDT"
     gene            complement(2613..3428)
                     /locus_tag="VGA59_09715"
     CDS             complement(2613..3428)
                     /locus_tag="VGA59_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9719995.1"
                     /translation="MKVFVITGASDGIGAELARQLAAAHGAQAGLMLAARNRDKLEAV
                     AAQCQVRGARAEVAQINVSEEPQCRALIDMAVQAFGRIDVLINNAGVSAHALFADVKA
                     QDLHWYENLMAINLWGSVWCTHAALPHLKASGGQIVAVSSVAGLIGVPGRTAYSASKF
                     AMTGFFESLRAELKSSGVGVTIAYPGVVATAIRYRGYNAKGEAAGVSGLKEEKAMSVE
                     QCARLIIEGMNRRRREIVMTAGARLGRFAKLIVPGMVEKVALAALKEEVRPRK"
     gene            complement(3531..3662)
                     /locus_tag="VGA59_09720"
     CDS             complement(3531..3662)
                     /locus_tag="VGA59_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9719996.1"
                     /translation="MTVRTRKLLAYAAAIAVLLAVFALYTRPLIMVTLAEQIWACFN"
     gene            complement(3665..4882)
                     /locus_tag="VGA59_09725"
     CDS             complement(3665..4882)
                     /locus_tag="VGA59_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9719997.1"
                     /translation="MHNPAPRHLSMVQVLACGAAIVTLSMGIRHGFGLWLQPITMAQG
                     WSRETFAFAIAIQNLAWGFFGIFAGMVADRFGAFRVLIVGALLYGAGLAGMALSPNAF
                     VFSLTAGILIGAAQAGTTYAVIYGVIGRQIDPARRSWAMGVAAAAGSFGQFLMVPVEG
                     FLIAGFGWQEALLALSLFVLLVAPLAWGLREPGFAGGAAPVRDQTVVQALKEAFKYPS
                     FQLLMAGYFVCGFQVVFIGVHMPSYLKDKGLSPQVASYALALIGLFNVFGTYIAGSLG
                     QKLARRKILAFIYLARSVAIALFLWVPLSPLSVYVFSSVMGLLWLSTVPPTNATVAQI
                     FGVAHLSMLGGFVFFSHQIGSFMGVWLGGLLYDRTGSYDIVWYIAIALGVFAAMVNLP
                     VREAPIARMPQAA"
     gene            5039..7084
                     /locus_tag="VGA59_09730"
     CDS             5039..7084
                     /locus_tag="VGA59_09730"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX9719998.1"
                     /translation="MASKLPAEYSEVSIRVLKGLEPVKQRPGMYTRTDNTLHVIQEVL
                     DNAADESLAGYGKRIKTTLHTDGSVSVEDDGRGIPIGLHPEEKAPVIELVFTRLHAGG
                     KFDKKGGGAYSFSGGLHGVGVSVTNALAKRLEVVTHREGQVARMVFAGGDVAEKLQVR
                     KAQEGDRRQGTTVRVWPDPKYFESSQLPMAELAHLLRSKAVLMPGVTVSLVNEKTRET
                     QSWQYKGGLRDYLMQTLTGDPVIPLFEGEAFAANNDNFAEGEGAQWCVAFTEEGQPVR
                     ESYVNLIPTSAGGTHEAGLRDGLFTAVKSFVDLHSLLPKGVKLLPEDVFARASYVLSA
                     KVLDPQFQGQIKERLNSRDAVRLVSSFVRPALELWLNQHVEYGRKLAELAIKAAQSRQ
                     KAGQKVEKRKGSGVAVLPGKLTDCESRDLSHNEVFLVEGDSAGGSAKMGRDKECQAIL
                     PLRGKVLNTWEVERDRLFANNEIHDIAVAIGVDPHGPEDAPGLSGAGPSQAASATSGG
                     SDQRERGGDVLAGLRYGKICILSDADVDGSHIQVLLLTLFFRHFPKLIEEGRVFVARP
                     PLFRVDAPARGKKPASKTYALDAGELTAILDKLRKEGVREGAWNISRFKGLGEMSAEQ
                     LWDTTLNPDTRRLLPVHLGTQDFLQTESLITKLMGKGEAAARRELMELHGDAVEIDV"
     gene            7096..7656
                     /locus_tag="VGA59_09735"
     CDS             7096..7656
                     /locus_tag="VGA59_09735"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX9719999.1"
                     /translation="MTAANFAASKPDRTTTRVRLRPTMTSDLEYVLSLEQDPDNLAFI
                     TPWERTQHEAAIRFPDFRHFIVEGGEGLDAVGFLILIGCRSQNQSLELKRIVIQTKGS
                     GFGRAALRVAKKVAFDDLGAHRFWLDLKVRNTRAKALYDSEGFVVEGELRDAVKVAGG
                     FDSLVVMSMLRPEFTGRRSLGLELRE"
     gene            7653..8567
                     /locus_tag="VGA59_09740"
     CDS             7653..8567
                     /locus_tag="VGA59_09740"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9720000.1"
                     /translation="MKAAMFKAALAGLLLAALVPAARAEVWGYVDDNGVAHFASEKMD
                     ERYELFYRGGESFDTASGVGAARAMAVPTTAPKLVAFFEISPSFRQVKHHLLEASRVQ
                     GIDYELLKALIATESGFNTRAVSPKGAVGLMQLMPATAERYGVTGDRKTPLAKKLTDP
                     RTNIKTGVRYLRYLIDLFPGRLDLALAAYNAGEGAVQRAGNKVPKYRETQNYVKTVMQ
                     LYTILKPPSMTPRHLKGRTPGRVRVQLPGPPNGGVYAPATIIGGAANRGNLPSRPLRQ
                     SQPTTVTTPGSAFDLAAAMQGESRTERD"
     gene            8622..9764
                     /locus_tag="VGA59_09745"
     CDS             8622..9764
                     /locus_tag="VGA59_09745"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9720001.1"
                     /translation="MTPVPADPDQLVALGAFAGAQVLVVCFSVIVANTYRERALLLHG
                     AATMMGVLAVQSLVGGRPFLAEAAVLLVLALDGMQLRDLVSHTGALRQPRRWLAGISL
                     LLMPALALAIFLGERHLLLPGLAAWIAVVVVIMMRVWSQSQPWAWWLVPGQLALAAGA
                     GWLGWRSLDAEPDPAVPLAALLSLWAAAIFLATAWRSRIFSETRLRIDARNTVDPLTG
                     LSTPLIFYDRVRAVRSLMARYGHPSVLLLVHIEDLQKLSARFGPELAESALLVAASRI
                     REALREGDVAARLSHSRFGVLAEGMSLAEGSANVASRILVAGLKEPLPAAPTEFLHFR
                     IVLATVPESDVPAKVLLQRMAARMDEQLLAPSERRIVALAAEELLA"
     gene            9799..12123
                     /gene="parC"
                     /locus_tag="VGA59_09750"
     CDS             9799..12123
                     /gene="parC"
                     /locus_tag="VGA59_09750"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX9720002.1"
                     /translation="MEQDLIPLANGDDAELDLASYAQRAYLEYALSVVKGRALPDVCD
                     GQKPVQRRILYSMARMGLGFTGAAGARPVKSARVVGDVLGRFHPHGDQAAYDALVRMA
                     QDFSQRYPLIDGHGNFGSRDGDGAAAMRYTEARLARITNLLLDEIDEGTVAFIPNYDG
                     STQEPQQLPARLPFTLLNGASGIAVGLATEIPSHNLREIADACMALIKTPKLSDEDLF
                     ALVPGPDYPGGGQIISASSDIAEAYRTGRGSLKVRARWKIEELARGQWQLVVTELPPG
                     VSTQRVLEEIEELTNPKVKAGKKGLSQEQVQLKQTVLAVLDAVRDESSKDAAVRLVFE
                     PKTSRIEQSELVTALLAHTSLETSSPVNLTMVGLDGKPVQKSLRLMLSEWIEFRQATI
                     ERRSRHRLGKVMDRIHILEGRQLVLLNIDEVIAIIRKSDEPKAALVARFKLSDRQAED
                     ILEIRLRQLARLEAIRIEQELKELREDKKKLDEILGSPAALRRLMVKEIEQDAKQFAD
                     ARRTLIQADRKVVAEIRVIDEPVTVVLSQRGWVRARQGHGHEAASFAFKAGDGLYATF
                     ECRTVDTLLAFGSNGRVYTVPVSMLPGARGDGQPVTTLVDLEAGTHLLHYFAGAAAAW
                     LLLSGSGGYGFLATVENMMSRLRGGKAFISCGNGETVCRPSPASVPPQTPATHVACAS
                     TGGRILTFEIGELKPMANGGRGLMLIDLDPKDTLAGAAAYTRSVKISGIGRGGKEREE
                     TLEIRSLNNAKAGRGRKGKMADLGFKPTSIERVE"
     gene            12120..13652
                     /locus_tag="VGA59_09755"
     CDS             12120..13652
                     /locus_tag="VGA59_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX9720003.1"
                     /translation="MSSPAAACGAAPPFRAIPDLVREHAAARPDRTALIEGGRSVTWG
                     QVDAMADRVAASLQRDGLEPGATIAICSSNSLEYVAVFLGALRAGVTVAPLATQSSPE
                     QLAGMVVDSGTGHFFVDRGVPAFDTTARRIFLDGSDTPSLEQWLAAAGTRPRRVEIQP
                     QWPFNIIYSSGTTGAPKGIVQSHAMRWAHVARAENYGYGPEAVTLIATSLCSNTTLVC
                     FFPTVARGGCVVLAPPRFDAAAYLALARKTRATHTMLVPVQYRRIMALPDFGRYDLSS
                     FQMKFCTSAPFHAALKADILKRWPGGLVEFYGMTEGGGSCLLEAHNFPHKLDTVGKPP
                     EGHDMRVIDEEGRELAAGAVGEVVGRSPSMMTGYHGRPERTREAEWNDAQGNRFIRTG
                     DVGRFDADGFLTLMDRRKDMIISGGFNIYPSDLEAVLRRHAGVADAAVVGVASAQWGE
                     TPVAFVVPAADAPREEELRAWANAQLGKTQRLADVRYIDELPRSDIGKVLKRQLRDAY
                     KG"
     gene            13732..14403
                     /locus_tag="VGA59_09760"
     CDS             13732..14403
                     /locus_tag="VGA59_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9720004.1"
                     /translation="MDKRRIKDLLYEQVARIGKALASPKRLELLEVLAQGEKTVEALS
                     AELSIDFKLASSHLKALRASRLVESRRDGKYVLYRLSGPDVSDLWVNLREVAEEHLLE
                     LRMAIDQMVAQPDKLSKVDRKALLDQAKRGEVIVIDVRPQGEYETAHLPFARSMPLEE
                     LEKRLSELPRRREIVAYCRGPFCMLSDEAIKLLHARGYKARKILDGVIEWQAAGLPVT
                     SGKPA"
     gene            14508..15239
                     /locus_tag="VGA59_09765"
     CDS             14508..15239
                     /locus_tag="VGA59_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9720005.1"
                     /translation="MFFKQLATKEASLSYFFGCIGHRKAIAVDVVAGDESWFVDQAKA
                     LDVQIAFVVDTHLHADHYSGGRALAALTGAPYCLHEAACELVRFDFVSLRDGQELDAG
                     NVRAKVLHTPGHTLDSICLLVKDMRRGDEPWFVVTGDTLFVGAVGRPDLAGRETEMAG
                     MLYDSLHEKLLTLPGEMEVFPAHQSGSACGVGLSGKPSSTLGFERRWNPALSMDRAQF
                     IDSITQGIPPRPAGMEAIVAANTAA"
     gene            15246..16556
                     /locus_tag="VGA59_09770"
     CDS             15246..16556
                     /locus_tag="VGA59_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9720006.1"
                     /translation="MELQHGIRANLSQFLHQLLQVLLVGLTIGMMRTVVPALAESEFA
                     VPRNSFLLLSAFVVAFGIVKGVMNFVAGRLSERVGRKKVLLLGWIAAAPIPFMVWYAP
                     TWNWVVAATVLLGVNQGLTWSMTQTAKLDITKPDERGLTLGLNEFAGYLGVALAGVAT
                     AYLAETLGARVGLLWFGVVVIGLGLLLTGVSVRDTLAWAHAEADRHRAPAAPGQSRPR
                     YPEGVSAHPGTWEIFTLMSWRDRRLAALSQAGLVEKFVDALVWVLYPVFLYQQGLSLP
                     QIGWITGVYGFVWGGSQLVTGRLSDRIGRHKPNVWGMWICGAGVLMMVLGSGPVWWAL
                     SAGVSGLGMALLYPNLSAAVADISHPAWRGSAIGIYRFWRDLGYGVGALGLALAAQFT
                     GRLEAAFWFVGISMLLSGAVLAALGEETHPRLNPAYSGHLPQKT"
     gene            16791..17933
                     /locus_tag="VGA59_09775"
     CDS             16791..17933
                     /locus_tag="VGA59_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX9720007.1"
                     /translation="MDEPIYLDYNATTPVAPEVVDAMLPFLRQHFGNPSSSHVYGQRA
                     AAAVAEARSQVAALVGARPAEIVFTGCATEANNLALLGVARALGEAKRHLVVSAVEHP
                     AVMAPAARLQEQGWALTVVGVDQFGRVSPDEVQRALRPDTAMVSVMHANNEVGTIQPI
                     QEIAQLTRARGIVLHVDAAQSAGKIPVNVDALGVDLLTLAGHKFYAPKGIGALYVRAG
                     TPLKSVAFGAEQEAGLRPGTENVPGIVGLGAAALLARVGLGEVNARLRKLRDRLHTLL
                     LESIPGLGLNGHPEHRLPNTLHVSFPGVSGRALLLEAAGIVAASVGSACHSEHDAVSG
                     VLAAMGADASRASGAVRLSVGRTTTEDDISRAASGLTSAWIRMAGR"
     gene            18028..18378
                     /locus_tag="VGA59_09780"
     CDS             18028..18378
                     /locus_tag="VGA59_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX9720008.1"
                     /translation="MKTLFILNDAPYGTERSYNGMRLARHLLKTGSEEVKVFLMGDAV
                     ACAKAGQKVPQGYYNAGDMLGMIVRAGGEISVCGTCIDARGISAEELIEGASRGGMDV
                     LTRWSQWADKVVTF"
     gene            18384..19547
                     /locus_tag="VGA59_09785"
     CDS             18384..19547
                     /locus_tag="VGA59_09785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX9720009.1"
                     /translation="MSRTALVLGAGIGGLVAAETLRKLLPPSDRVIAVDRAADHFLAS
                     SLLWLMVGQREPADFTRPLGRLAERGVDLRCGNVTRIDPGRCAAEIDGEAISADALVI
                     ALGADYAPEAIPGLAVAGLNLYAMEGAIAIRDALARFEGGRIVIMTAAPAYKCPAAPY
                     EAAMLVDSCLRKQGVRDKTTMFFFAAEPQPMVVTGPEIGAAVRGMIEARGIVYRPEHQ
                     VEEVNPSTRQINFTNGVAARFDLLIYVPPHRAPAVARNAGLTNDSGWIPVDRHTLQTS
                     FENVFAIGDITSVPLKMGRPLPKAGVFAHGQAEVVANNIARAWTGKGSSRQFSGQGMC
                     FIETGDGRAGIGKGNFYAEPTPEVEMHGPNLLWHAGKVLYEKYWLYRRFRAGL"
     gene            19581..21917
                     /locus_tag="VGA59_09790"
     CDS             19581..21917
                     /locus_tag="VGA59_09790"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9720010.1"
                     /translation="MFNFSLVDFSIRRPKLIVWATVVVTLLFLTQFAGLTTDTNPKHM
                     LPETSDVRVWNDEVDKTFSLYEDTIAVGVHNEAGVLNRETLGRIARITDAILKLEGVA
                     SRDVNGFTTITNVTAEGGTLKVGPLMPGAPKTDAEVEALRAALFGNPLLQGRVISRDG
                     KTTAIYVPLEKGANGADIAQKIRGVVSAEKGPERYYIAGDPVARDTFGAEMFKLMGIF
                     SPIAGMIMFAAIQFMFRSLALSFAMMAVAMAAIIWSMGLLIGAGFPVHIMSSMAPVFL
                     MAIATDSIHIFNEFYFRYRERRDKLAAIRETMQAVSRPVRNTALATAAGFAVLLFMDI
                     VPVQVFGGLIAFGTIVLRLFSFSLLPAILTFVSEEKIARAADHESMELDRSARLLRRI
                     GSIGADHPGTTALVGLALVIAAIVGTSKIHINNNMVAWFKESSEIRVADTALNRALGG
                     TSLGYLVVQSTEPDFMKRPEAMRWLEGLQRHLETSPVVGKTFSVADYVKRINLVLHDN
                     DAQFAAVPQTQEMVGQYLFLFSMSAKPSDLDNVVDPGFRKANVWVQLKTWDADAMRQV
                     IQSAQTYQKANPLAVTVKPAGTAYFNLVWNDEVLGDMVLGLGLALVVVFVILALDFRS
                     ARWALVGYVPLLFTILLIYGVVGFAGKDFDMPLSVLSSLSLGMAVDFAIHFISRFRQR
                     LAEAGGDVREALLWTAARPGKGIVRNALLFAAAFSVMLFAPLTPYIAVGAFIAGMMLL
                     SALFTILYLPALVMLGRGWLLDQPMPAAQPVPAFAKGQ"
     gene            21919..22707
                     /locus_tag="VGA59_09795"
     CDS             21919..22707
                     /locus_tag="VGA59_09795"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX9720011.1"
                     /translation="MNRWSLWILASLLPLQALALTGEEVMRKSQAAFFYPGKDFKARV
                     VMKLINKDGQERVREMTMLRKNAGEPGGDQKYFMYFFQPADVKDMSFMIHKYPARDDD
                     RWMFIPAINMVKRIAAQDKRSSFVGSDFTYEDVSGRDLEDDSHVLEREEKVGQREVYV
                     VKSTPKGADADFGYKFTWVDKANFLPLKELQHDRKGAAYKQFSADEVADIKGQPTVIK
                     RTMRNVQTGHRSEVSYLKADYDMGIEDSLFSERFLRQPPRKWVD"
     gene            22707..23933
                     /locus_tag="VGA59_09800"
     CDS             22707..23933
                     /locus_tag="VGA59_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720012.1"
                     /translation="MRHAIGCAVALLGALAPLAARADVSLSGFLQQNTAVNTASANPD
                     GRHQKWLEERAQLKLDAAGGAWRLLAKGDVAYDHLGRQEQSELREGYIDYTAGNWDLR
                     LGRQVITWGLGDLVFVNDVFPKDHEALFAGRPLEYLKRGVDAVKFGLYPQLASFELVV
                     APRFRESTIPDARRFHLFDPMPAAANRETVTPGKGEAGLRIYRDIAGYDAALYLYRGF
                     HRTPSMRPDSATDPTQITFFHPGLSVYGASVSGRAGEGVASLETAYYDSREDRAGSDA
                     AVPNSQARLLVGYQIQPLEDLSLGLQYYGEYMRHYAAYRASLPAGFPVDKRWSHTITA
                     RLTQFFLHQTLRLSVYASYNGANGDRFLNPELRYSFTDKVWGAVGANLFDGKPWGQFG
                     QLARDDNVYLQVRYEF"
     gene            complement(24096..>24313)
                     /locus_tag="VGA59_09805"
     CDS             complement(24096..>24313)
                     /locus_tag="VGA59_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9720013.1"
                     /translation="GDLGKVKRIVKLMSLVNSTGDFTEHHLVTNGASELLGEVFGDEG
                     THARSAFGVAQIPTGACVEIELIAEIG"
BASE COUNT         4214 a         7823 c         8135 g         4141 t
ORIGIN      
        1 cgacataccg cacgaaatac atgccctgcg cgcggtcccg gtcctcgacg gtgaatccgg
       61 tgcggtccag ggtcaggccc acgcggcgcc aggcgcggtc gaacccttca tcgatctgca
      121 ccaccggcgt gttgccgacg gtggcgacgc gcgaagtggt cttggcggct cccgaggcga
      181 cgagggcctt cgattgctcc tggggtacgc cgagcttgac catcaggcgg cgcaggaatt
      241 ccgcttcgag ctcgggatcg gaaggacgcg gctgccacac cgtctggtcc ttctgcgtgt
      301 tgctgtagac ctcgatcatg ccgcggtggc tgacatagat ttcggtgccg ccgctggcgt
      361 tgcgctccag tcgggtgcgg aagcggtcgc gttccgccgt ggagtagagc gagtcgaata
      421 ctttgccgag cgcgttgcgg atgaagtcct gcggcagctt ggcccggttt tcggcccagt
      481 ccgtttccat gatgcccagg ctggcctggt ccagggccag caggaacccg ctttcctgcc
      541 agaactcgcg caccggattc cacaactcgt cggccgagcg gttcacgacc agccagcgct
      601 gggtgccggc gcgctcgatg cgcacgtcgc ccagggtcga cgccgcggtg ggcacgccgg
      661 gagcggcctg cccgacctgg aagctgctgg cggtgacggg tccgcccggc accgtgtagc
      721 gcgaatcgcg cgacagctgc gtgaggtcgg gcggcacctc cagcgaagga cccttgccgg
      781 cgctcttgta gtcgatcttt tcgccttcca ggatcgtgca ggccgagagc gcggcgcaca
      841 tcgccaccac gccgaatctt gcaaattgct tcacgtcatt gccttcagga ttggattgtc
      901 gggattcaag ccagcaggcc ggccgcgcgc agcgcgcctt cgaccgccgc ctcattggcg
      961 cgcgccagcg tcgtcatggg caggcgcagc gccccgccgc acaggcccat gcgggccatt
     1021 gcccatttga ccgggatcgg attggcttcg acgaacaggt tcttgtgcac cggcatcagc
     1081 ttgcgctgga tttccatggc gcgcctggca tcgccggcca cggccgcgac gcacagctcg
     1141 tgcatcaggc gcggcgcgac gttggcggtc acgctcacat tgccctgccc accgcagagc
     1201 atcagcgcca cggcggtggg atcgtcgccc gaatacacgg agaatccctt gggcacctcg
     1261 cggaccagcc attgggcgcg ctcgatgttg ccggtggctt ccttgatgcc gacgatgccc
     1321 ggcacctgag ccagccgcag caccgtgtcg tgcagcagat cggccacggt gcgtccgggc
     1381 acgttgtaga ggatgatggg caggtccacc gcctcggcga tggccttgaa gtgcaggtat
     1441 tggccttcct gggtcggctt gttgtaatag ggcacgacct gcagctggca gtcggcgccg
     1501 atcttcctgg cgaacttcgc cagctcgacc gcctcggctg tcgagttggc gccgcaaccg
     1561 gccatgatgg gcacccgctt gttggcctgc tcgaccgcca cacggatgat ttcatggtgc
     1621 tcctgcacgt tgaccgtggg cgactcgccg gtcgtgccga ccacgccgat gcagtcggtc
     1681 ccttcggcga tgtgccagtc aatgagcttg cgcaacgtgg ggtagtccac gctgccgtcg
     1741 tcgtgcatcg gggtggccag cgccacgatg ctgcctgtaa tcggtgtcat gtccggagtt
     1801 tcccgtcgaa agaccaattc taactagcca tgcccggccg cctgaaacgg ctacgtgtcc
     1861 tgctcaggcg cggcgatgcc cggcggatgt tgcagccggt agcgcggccg tggctcaccc
     1921 ggcgccggcc gcacggcggc gattctctgg acgaaccgtg ccggctcgtc caggaagccg
     1981 tcttcgaagg cgaccacctg cagttcgctg caggcgcgca gcagttcgcc cggctgcaac
     2041 aggaagtcgg gccgcgacgg cttgcccacg ctctcgtggc ccgccgtgaa ggtttcatag
     2101 atcagcgcgc cgcccggcgc cacgctttgc aagagagtgg gccacagcgg ccgccacagg
     2161 taattcgtga cgacgacgcc gccgaaggtg cggctcggga agggccaggg accgttctcg
     2221 atgtcggcca gcacggcttc gcccaggctt gacacggcat cgatcgcttc cggggcccgg
     2281 tccacgccgg tgacgggatg gccacgctgt gaaaaccagc gcatgtgccg gccaaagccg
     2341 caagcgacgt cgagcacggc gccgccggcg gggaccaggt gggtccaacg ctggacccag
     2401 gcggaggggg attcggttcc gtgcatggtc gtggtgcctg cgccatgggg catggacgga
     2461 ttgggcatct cagtttccca tgaaggagaa cttcacccac gacggatgaa agtcgattct
     2521 gtaggcgcgg gccaccgcgg aaccggcttt gccgggccgc tggtggcgcc accttgaggg
     2581 ggaggcgccg aaggcgcttc gggggtgggc tttcacttgc ggggccggac ttcttccttg
     2641 agggccgcca gcgccacctt ctcgaccatg ccggggacga tcaatttcgc gaagcggccc
     2701 agcctggccc ctgccgtcat gacgatctcc cgccggcgcc ggttcatccc ctcgatgatg
     2761 agccgggcgc attgctccac gctcatcgct ttttcttcct tcaggccgct gacgccggcg
     2821 gcttcgccct tggcgttata gccgcggtaa cggatggccg tggccaccac gcccgggtac
     2881 gcgatggtca cgcccacgcc gctgctcttg agctcggcgc gaagcgattc aaaaaatccc
     2941 gtcatcgcga atttgctcgc actgtaggcc gtacggccgg gcacgccgat caggccggcg
     3001 accgaggaaa ccgccacgat ctggcccccg ctggctttca ggtgcggcag cgcggcatgg
     3061 gtgcaccaga cgctgcccca caggttgatc gccatgaggt tttcgtacca gtgcaggtct
     3121 tgcgccttga cgtcggcaaa cagggcgtgc gccgacacgc cggcattgtt gatcagaacg
     3181 tcgatgcgcc cgaatgcctg gaccgccatg tcgatcaggg cccggcactg gggctcctcg
     3241 gaaacgttga tctgcgcgac ttcggctcgc gcgccgcgga cctggcactg cgccgcgacg
     3301 gcttcgagct tgtcccgatt gcgcgccgcg agcatcaggc cggcctgcgc gccatgcgcg
     3361 gccgccagct ggcgtgccag ttcggcgccg atcccgtcgg atgcgccggt gatcacgaaa
     3421 actttcatgc gcggattctc actcatgctt tgtcattgcg ggcttgaccc gcaatccagg
     3481 gggcgctcag taggacatgg atgccgggtc aagcccggca tgacgaccgg ctagttgaaa
     3541 caggcccaga tctgctccgc gagtgtgacc atgatgagcg gtcgcgtata gagagcaaac
     3601 accgccagca gcaccgcgat ggcggcagcg taggcgagga gtttgcgcgt gcgaaccgtc
     3661 atcgtcacgc tgcctgcggc atccgggcaa tcggcgcttc gcgcaccggc aggttcacca
     3721 tcgccgcgaa gacgccgagc gcgatcgcga tgtaccagac gatgtcgtag ctgccggtgc
     3781 ggtcgtaaag caagccgccc agccacacgc ccatgaagga gccgatctgg tggctgaaga
     3841 acacgaagcc accgagcatg gacaggtgag cgacgccgaa aatctgcgcc acggtggcgt
     3901 tggtcggagg cacggtggac agccagagca gccccatcac gctggagaaa acgtaaaccg
     3961 acagcggcga gagcggcacc cagaggaaca gcgcgatcgc caccgagcgg gccaggtaga
     4021 tgaaggcgag gatcttgcgg cgggcgagtt tctgccccag gctgcccgcg atataggtgc
     4081 cgaacacgtt gaacagcccg atcagtgcga gcgcgtagct ggcgacctgg ggcgacaggc
     4141 ccttgtcctt caggtagctg ggcatgtgca cgccgatgaa cacgacctgg aaaccgcaga
     4201 cgaagtagcc ggccatcagc aactggaagc tcgggtactt gaaagcttcc ttcaatgctt
     4261 gaaccacggt ctggtcgcgc accggcgcgg cacctcctgc gaagccgggc tcgcgcaggc
     4321 cccaggcgag tggcgccacc agcaggacga acaggctcag cgccagcaat gcttcctgcc
     4381 atccgaagcc ggcgatgagg aagccctcca ccggcaccat caggaactgc ccgaaggatc
     4441 cggccgcggc cgccacgccc atggcccagg atcgcctggc cggatcgatc tggcggccga
     4501 tcaccccgta gatcaccgca taggtggtgc ccgcctgtgc cgcgccgatc aggatgccgg
     4561 cggtcagcga gaacacaaag gcgttgggcg acagcgccat gccggccagt cccgcgccgt
     4621 agagcagcgc gccaacgatc aacacgcgaa aggcgccgaa gcggtcggcc accatgccgg
     4681 caaagatgcc gaaaaagccc caggccagat tctggatggc gatggcgaac gcgaatgtct
     4741 cccgcgacca gccctgcgcc atcgtgatgg gctgcagcca caagccgaag ccgtggcgga
     4801 tgcccatcga caatgtgacg atggcggcac cgcaggccag tacctggacc atggagaggt
     4861 ggcggggggc gggattgtgc atccggcgac tttatcgaaa gccgaccgta gccgcccccg
     4921 caagggcaaa aggcctgttg aattttctgg agcgcattga tgcggcccag gcatgaatgg
     4981 acttgaatct gtatgcccat acagtggttg aaggcggccc gactacaatc gacctcccat
     5041 ggccagcaaa ctccccgccg aatattccga agtttcgatc cgcgtcctca agggcctgga
     5101 gcccgtcaag cagcggccgg gcatgtacac gcgcaccgac aacacgttgc acgtgatcca
     5161 ggaagtgctg gacaacgccg cggacgaatc cctggccggt tacggcaagc gcatcaagac
     5221 cacgctgcac acggacggtt cggtcagcgt ggaggacgac ggccgcggca tccccatcgg
     5281 cctgcacccg gaagagaagg caccggtgat cgagctggtg ttcacacgtc tgcatgccgg
     5341 cggcaagttc gacaagaaag gcggaggcgc gtacagcttc tcgggcggcc tgcacggggt
     5401 cggcgtgtcg gtcaccaacg cgctggccaa gcgtctcgaa gtcgtcacgc atcgcgaggg
     5461 gcaggtcgca agaatggtgt tcgcgggcgg cgacgttgcc gagaaactgc aggtgcgcaa
     5521 agcccaggag ggcgaccggc gccagggcac gacggttcgc gtctggcccg atccgaaata
     5581 cttcgagtcg agtcagctcc cgatggccga gctggcccac ctgctacgca gcaaggccgt
     5641 gttgatgccg ggcgttacgg tctcgctggt caacgaaaag acccgcgaaa cgcagagctg
     5701 gcaatacaag ggcggactgc gcgattacct gatgcagacg ctgacgggcg accccgtcat
     5761 cccgctgttc gagggcgagg cgttcgctgc gaacaacgac aacttcgccg aaggcgaggg
     5821 cgcgcagtgg tgcgtggctt tcaccgagga ggggcagccg gtgcgcgaaa gctatgtgaa
     5881 cctgattccc accagcgcgg gcggcacgca tgaagcgggc ctgcgcgacg gcctgttcac
     5941 cgccgtgaag agcttcgtcg acctgcattc gctattgccc aagggggtga agctgctgcc
     6001 cgaggacgtg ttcgcccgcg cctcctacgt cctgtcggcc aaggtgctgg acccgcagtt
     6061 ccagggccag atcaaggaac ggctgaattc gcgcgacgcc gtgcgcctgg tgtccagctt
     6121 cgtgcgtccc gcgctcgagc tgtggctcaa ccagcatgtg gagtacggcc gcaaactggc
     6181 cgaactcgcc atcaaggcgg cgcaaagccg gcagaaggcc ggccagaaag tcgagaagcg
     6241 aaagggctcc ggcgtggccg tgctgccggg caagctcacc gactgcgaaa gcagggactt
     6301 gagccataac gaggtgttcc tggtcgaggg cgactcggcc gggggcagcg ccaagatggg
     6361 acgcgacaag gagtgccagg cgatcctgcc gctgcgcggg aaggtgctca acacatggga
     6421 ggtcgagcgt gaccggttgt tcgccaataa cgagatccac gacatcgcgg tcgccatcgg
     6481 cgtggacccg cacggccccg aggacgcccc cggactgagc ggcgccgggc cgtcccaagc
     6541 cgcgagcgcc acctcggggg gcagcgacca acgggagcgt gggggggacg tattggccgg
     6601 cctgcgctac ggcaagatct gcatcctgtc ggacgcggac gtggacggat cgcatatcca
     6661 ggtgctgctg ctgaccttgt tcttccgcca tttccccaag ctgatcgagg aaggccgggt
     6721 cttcgtggcg cggccgccgc tgttccgggt ggacgcgccc gcgcgcggga aaaaaccggc
     6781 ttccaagact tatgcgcttg acgcgggcga attgactgct atcttggaca aattgcgcaa
     6841 ggagggtgtg cgcgaggggg cctggaacat cagccgcttc aaggggctgg gggagatgag
     6901 cgcggaacaa ttgtgggata cgacgctcaa tcccgatacc cgccgcctgc tgccggtcca
     6961 tctgggcacg caggacttcc tgcaaaccga gagcctcatc accaaactga tgggcaaggg
     7021 cgaagccgcc gcccgccgcg agctgatgga actgcacggc gatgccgtgg aaatcgacgt
     7081 ttgagcaaga tttgcatgac cgctgccaac ttcgccgcca gcaaacccga ccgcaccacc
     7141 acgcgggttc ggctgcgccc gacgatgacg agcgatctgg aatacgtgct ttcgctggag
     7201 caggatcccg acaacctggc gttcatcacc ccttgggagc gaacgcagca tgaggcggcg
     7261 atccgctttc ccgatttccg ccacttcatc gtcgaaggcg gagagggcct ggatgcggtg
     7321 ggctttctca tcctgatcgg ctgtcgcagc cagaaccagt cgctcgagct caagcgcatc
     7381 gtcatccaga ccaagggctc ggggttcggg cgcgcggcgc tgcgggtggc caagaaagtg
     7441 gcgttcgacg acttgggcgc gcaccggttc tggctggact tgaaggtgcg caacacgcgc
     7501 gccaaggccc tgtacgacag cgagggtttc gtcgtggaag gcgaactgcg cgacgcggtg
     7561 aaagtagccg gcggcttcga ttccctggtc gtgatgtcga tgctgcggcc cgaattcacc
     7621 ggacggcgca gcctgggact ggagctgcgc gaatgaaagc ggcgatgttc aaggccgcgc
     7681 tggcggggtt gctgctggcg gcgctggtgc cggcggcgcg ggcggaggtc tggggctatg
     7741 tcgacgacaa cggtgtcgcc catttcgcct ccgagaaaat ggacgaacgc tatgagctgt
     7801 tctaccgtgg tggggaaagc ttcgacaccg cgagcggcgt gggagcggcc agggcgatgg
     7861 cggtgccgac gacggctccc aaactcgtcg cgttcttcga gatctcgccc agcttcaggc
     7921 aggtcaagca ccacctgctc gaagcatcca gggtccaggg gatcgactac gaactgctga
     7981 aagcgctcat cgccacggag tccggcttca atacccgcgc cgtgtcgccc aagggcgccg
     8041 tcggcctgat gcagctcatg ccggccaccg ccgagcgcta tggcgtgacc ggcgacagga
     8101 aaacgccgct ggcgaaaaag ctcaccgatc cccggaccaa catcaagacc ggcgtgcgct
     8161 acctgcgcta cctgatcgac ctgtttccgg gccggctcga cctggcactg gcggcctaca
     8221 acgccggcga aggggcggtg cagcgggccg gcaacaaggt gccgaagtac cgcgagacgc
     8281 agaactacgt gaagacggtg atgcagctct atacgatcct caagccccct tccatgacgc
     8341 cgcgccacct caagggccgc acgcccggcc gcgtgcgcgt gcagttgccg ggcccgccga
     8401 acggcggcgt gtacgctccg gcaacgatca tcggcggggc ggcgaaccgg ggcaatcttc
     8461 cgtcgcggcc tttgcggcag tcccagccga cgacggtcac gacgccgggg tcggctttcg
     8521 acctggcggc ggccatgcaa ggcgaatccc gcaccgagcg tgactgaaac tgccggtccg
     8581 caccgcgcag taccctgccg ccctcccaga gggcccgccg aatgacgcct gtgccggccg
     8641 atcccgacca gctggtggcg ctgggcgcgt tcgccggcgc ccaggtgctg gtcgtgtgtt
     8701 tcagcgtgat cgtcgccaac acctaccggg agcgggcgtt gctgctgcac ggcgcggcca
     8761 ccatgatggg cgttctggcc gtgcaatcgc tggtgggagg cagacccttc ctcgccgagg
     8821 cggcggtgct gctggtcctg gcgctggacg ggatgcagtt gcgcgacctg gtcagccaca
     8881 ccggcgccct gaggcagccg cgtcgctggc tggccgggat cagcctgctg ctgatgccgg
     8941 cgctggcgct ggcgattttc ctgggcgagc ggcacctgct gctgcccggg ctggcggcgt
     9001 ggatcgcggt ggtggtggtg atcatgatgc gggtgtggtc gcaaagccag ccgtgggcct
     9061 ggtggctcgt gccgggccag ttggcactgg cggccggcgc cggctggctg ggttggcgct
     9121 cgctggacgc cgagcccgat ccggcggtgc cgctcgcggc cctgctgagc ctgtgggcgg
     9181 ccgccatctt cctcgccacc gcctggcgca gccgcatctt cagcgaaaca cgattgcgca
     9241 tcgatgcgcg caataccgtg gacccgctca cgggcctgtc caccccgttg atcttctacg
     9301 accgggtgcg cgcggtgcgc agcctgatgg cgcgttacgg ccatccgagc gtgctgctgc
     9361 tggtgcacat cgaagacctg caaaaactgt ccgcccgatt cgggcctgaa ctcgcggagt
     9421 cggcgctgct ggtcgcggcc agccgcatcc gcgaggcgct gcgcgaaggc gatgtggccg
     9481 cccgactgtc gcactcgcgc ttcggcgttc tggccgaagg aatgtcgctg gccgaaggct
     9541 cggccaacgt cgccagccgc atcctggtgg ccggcctgaa agagcccttg ccggcggcgc
     9601 ccaccgagtt cctgcacttt cgcatcgtgc tcgcgacggt gcccgagagc gacgtgccgg
     9661 ccaaagtcct gttacaacgc atggccgccc ggatggacga gcagttgctg gcgccgtccg
     9721 agcggcgcat cgtcgcgctt gcggccgaag agttgctggc gtgaagtttt ctttttttgt
     9781 tttttcctgc ctgaccccat ggagcaagac ctgattcccc tagccaacgg cgatgacgcc
     9841 gaactggacc tggcctcgta tgcgcagcgc gcttacctcg aatacgccct cagcgtcgtc
     9901 aagggccgcg cgctgcccga cgtgtgcgat gggcaaaagc ccgtccagcg ccgcatcctc
     9961 tattccatgg cgcgcatggg actgggcttt accggcgcgg ccggcgccag gcccgtcaag
    10021 agcgcgcgcg tggtgggcga tgtgctgggc cgctttcatc cgcacggaga ccaggcggcg
    10081 tacgacgcgc tggtgcgcat ggcccaggat ttttcgcagc gctatccgct gatcgacggc
    10141 cacggcaatt tcggcagccg cgatggggac ggcgccgcgg cgatgcgcta cacggaggcg
    10201 cggctggcgc gcatcaccaa cctgctgctg gacgaaatcg atgaaggcac cgtggccttc
    10261 atacccaact acgacggcag cacgcaggag ccccaacagt tgcccgcgcg cctgccgttc
    10321 accttgctca acggcgcgag cggcatcgcc gtcgggttgg ccaccgagat ccccagccac
    10381 aacctgcgcg agatcgccga tgcctgcatg gcgctgatca aaacgcccaa gctctccgac
    10441 gaggatctgt tcgcgctcgt accggggccg gactaccccg ggggcgggca gatcatcagc
    10501 gcctcgtccg atatcgcgga ggcttatcgc acgggccgcg gttcgctcaa ggtgcgcgcg
    10561 cgctggaaga tcgaggagct ggcccgcggc caatggcagc tggtggtcac cgagctgccg
    10621 cccggcgtca gcacgcaaag ggtgctggag gaaatcgaag agcttaccaa cccgaaggtc
    10681 aaagccggca agaagggcct gagccaggaa caggtccagc tcaagcagac cgtgcttgcc
    10741 gtgctcgatg cggtgcgcga tgaatcgagc aaggatgccg cggtgcgcct ggtgttcgag
    10801 ccgaagacca gccgcatcga acaaagcgag ctggtcaccg cgctgctggc ccataccagc
    10861 ctggagactt cgtcgccggt caacctgacg atggtcggcc tggacggcaa gccggtgcag
    10921 aagtcgctgc ggctgatgct gtcggagtgg atcgagttcc gccaggccac catcgagcgg
    10981 cgttcgcgcc atcgcctggg caaggtgatg gaccgcatcc acatcctgga aggccggcag
    11041 ctggtgctgc tgaatatcga cgaagtcatc gccatcatcc gcaagagcga cgagccgaag
    11101 gccgcgctcg tcgcgcgctt caagctgagc gaccgccagg ccgaggacat cctggaaatc
    11161 cgcctgcgcc aattggcgcg cctggaggca atcaggatcg agcaggagct caaggagctg
    11221 cgcgaggaca agaagaagct cgacgaaatc ctgggcagcc cggccgcttt gcgcaggctg
    11281 atggtcaagg agatcgagca ggatgcgaaa cagttcgccg acgcgcgccg cacgctgatc
    11341 caggccgata gaaaggtagt cgccgaaatc cgcgtcatcg acgaaccggt cacggtggtg
    11401 ctctcgcaaa ggggctgggt gcgcgcgcgc cagggtcatg gccacgaagc cgcgagtttc
    11461 gccttcaagg ccggcgacgg gctgtacgcc accttcgaat gccgtacggt cgacaccttg
    11521 ttggccttcg ggagcaacgg gcgcgtgtac accgtgcctg tatccatgct gccgggcgcc
    11581 cgcggcgacg ggcagccggt gaccacgctg gtcgacctgg aggcgggcac gcatctgctg
    11641 cactattttg ccggcgcggc cgccgcctgg ctgctcttga gcggctcggg cggttacggc
    11701 ttcctcgcaa ccgtggagaa catgatgtcg cgcctgcggg gcggcaaggc gttcatcagc
    11761 tgcggcaacg gggagaccgt ctgccgtccc tcgccggcca gcgtgccgcc gcaaacgccg
    11821 gccacgcacg tcgcctgtgc ttcgaccggc gggcgcatcc tgacgttcga gatcggcgag
    11881 ctcaagccca tggccaacgg cggccgcggg ctgatgctga tcgatctgga ccccaaggac
    11941 acgctggccg gcgcggctgc ctacacgcgc agcgtgaaaa tctccggcat cggccgcggc
    12001 ggcaaggagc gcgaggaaac gctggaaatc cgctcgctca acaacgccaa ggcgggacgc
    12061 gggcgcaagg gcaagatggc ggaccttggg ttcaagccca cttcgatcga gcgcgtggaa
    12121 tgagcagccc ggccgcggct tgcggcgcgg cgccgccgtt tcgcgccatc cccgacctgg
    12181 tgcgcgaaca cgccgcggcg cggcccgatc ggacggcgct gatcgaaggc gggcgcagcg
    12241 tgacctgggg ccaggtcgac gcgatggccg accgtgtggc ggcttcgctg cagcgcgacg
    12301 ggctcgagcc cggggccact atcgccatct gttcgtcgaa ctcgctggaa tacgtggccg
    12361 tattcctcgg ggcgctgcga gccggcgtga ccgtggcgcc gctggccacg cagtcgtcgc
    12421 cggagcagct tgccggcatg gtcgtcgaca gcggcacagg tcactttttc gtggaccgcg
    12481 gcgtgccggc gttcgacacg accgcgcggc gcatcttcct ggacggcagc gacacgccat
    12541 cgctggaaca atggctggcc gccgcgggga cgcggccgcg gcgcgtcgag atccagcccc
    12601 agtggccgtt caacatcatc tattcctcgg gtaccaccgg ggcgccgaaa ggcatcgttc
    12661 aatcgcatgc gatgcgctgg gcccacgtgg cgcgggcgga gaactacggg tacgggcccg
    12721 aggcggtcac gctgatcgcc acctcgctgt gttccaacac cacgctggtc tgtttctttc
    12781 cgaccgtcgc caggggcggc tgcgtggtgc tggcgccccc gaggttcgac gccgccgctt
    12841 acctcgccct ggcgcgaaag acccgggcga cgcataccat gctggtcccg gtgcagtacc
    12901 ggcgcatcat ggcgctgccc gatttcggcc gctacgacct ctcctcgttc caaatgaagt
    12961 tctgcaccag cgcgccgttc cacgccgcgc tcaaggcgga catcctgaag cgctggccgg
    13021 gcgggctggt cgagttctat ggaatgaccg agggcggcgg ctcctgcctc ctggaggcgc
    13081 acaacttccc gcacaagctg gacacggtgg gcaagccgcc cgaaggccac gacatgcgcg
    13141 tgatcgacga ggaggggcgg gaactggcgg ccggggcagt gggcgaggtg gtgggacgct
    13201 cgccttcgat gatgaccggc taccatggcc ggccggagcg cacgcgcgag gccgagtgga
    13261 acgacgcgca aggcaaccgc ttcatccgca ccggcgatgt cgggcgcttc gacgcggacg
    13321 gtttcctcac gttgatggac cggcgcaagg atatgatcat ctcgggcggc ttcaacatct
    13381 atcccagcga tctggaggcc gtgctgcgcc ggcatgccgg cgtggccgat gccgccgtgg
    13441 tcggcgtggc ttcggcgcag tggggcgaaa cgccggtggc cttcgtggtg ccggcggccg
    13501 atgcgccgcg cgaggaggag ctgcgcgcct gggccaatgc ccagctgggc aaaacccagc
    13561 ggctcgcaga tgtgcgctac atcgacgagt tgccgcgcag cgacatcggc aaagtgctca
    13621 agcggcaatt gcgcgacgcg tacaagggtt gagagatcaa ctcaacaagg gcacccagag
    13681 gacgcaagat tcaccggttg acatcattct aagtatcgct agaatgatcg aatggataag
    13741 cgtcgcatca aagatctcct gtacgaacag gtcgcccgga tcggcaaggc attggcgagt
    13801 cccaagcgcc tggaactgct cgaagtgctg gcccaagggg agaagacagt cgaggcgctc
    13861 agtgccgagc tgtccatcga tttcaagctg gccagctcgc acttgaaggc gctgcgggcg
    13921 tcgcgcctgg tggagagccg gcgcgatggc aagtacgtgc tgtatcgcct gagcggcccg
    13981 gatgtgtcgg acctctgggt caatctcagg gaggtcgcag aggaacatct gctcgagctg
    14041 cgtatggcca tcgatcagat ggtggcccaa ccggacaagc tttccaaggt cgaccgcaag
    14101 gccctgctcg accaagccaa acgcggtgag gtgatcgtca tcgatgtgcg tccgcagggg
    14161 gaatacgaga cggcccacct gccgtttgcg cgctcgatgc cactcgaaga actcgagaag
    14221 cggctgtccg agctgccgcg ccggcgcgag atcgttgcct actgtcgcgg gcctttttgc
    14281 atgctctcgg atgaggcgat caagctgctg cacgccaggg gctacaaggc caggaagatc
    14341 ctcgacggcg tgattgaatg gcaagccgcc ggcctgcccg tgacgtccgg caagcccgcc
    14401 tgaatcgcaa ctgatgaaag gaacatctgg tgcgtgctgg cggcaagtga caggtagctg
    14461 acgcctcggc gcagtcgagg tccaggcgtg ttctgaagga ggtgcaaatg tttttcaagc
    14521 agcttgcgac caaggaagcg tcgctgtcct acttcttcgg ctgcattggc caccgcaagg
    14581 ccattgccgt ggacgtggtg gccggcgacg agagctggtt cgtggaccag gcgaaggctc
    14641 tggatgtgca gatcgctttc gttgtcgata cgcatctcca tgcagaccac tactcgggcg
    14701 gtcgcgctct tgccgccctc acaggcgcgc cttactgctt gcatgaggca gcgtgcgagc
    14761 ttgtgcgttt tgattttgtg tcattgcgcg acggccagga gctggatgcc ggcaacgttc
    14821 gcgccaaggt tctccacacg cccgggcaca cgctcgacag catctgcctg ctggtaaagg
    14881 acatgcgacg cggagatgag ccctggtttg tggtgacggg ggacacgctc ttcgtcggcg
    14941 ccgtgggccg gccggacctc gccggccgcg aaaccgaaat ggccggcatg ctttatgaca
    15001 gcctgcatga gaagctgttg acgctgccag gcgaaatgga ggtgttcccg gcacatcagt
    15061 caggcagcgc ttgcggcgtc ggcctttccg gcaagccgtc atctacgctc ggcttcgaga
    15121 ggcgttggaa ccccgctctg tcgatggacc gtgcgcagtt cattgactcg atcacgcaag
    15181 gcatcccgcc gcgtcccgcc ggcatggaag ccatcgttgc ggcgaacacg gcggcgtagc
    15241 gaaccatgga actgcaacac ggcatccgcg ccaacctgtc gcagttcctc catcagctgc
    15301 tgcaggtgct gctggtgggt ctgaccatcg gcatgatgcg caccgtggtt cctgccctgg
    15361 ccgagtccga gttcgcggtg ccacgcaact cgttccttct gctgtcagca ttcgtcgtgg
    15421 cattcggcat tgtcaaaggc gtcatgaact tcgtggcggg ccggctttcg gagcgcgtgg
    15481 gccgcaagaa ggttctgctc ctgggctgga tcgccgccgc cccgatccct ttcatggtct
    15541 ggtacgcgcc cacatggaac tgggtcgttg cggctaccgt gctgcttggg gtcaaccagg
    15601 gcttgacctg gtcgatgacg cagacggcca agctcgacat caccaagccg gacgaacgcg
    15661 gcctcacgct cggcctcaat gagttcgctg gctaccttgg agtggcattg gcgggcgtgg
    15721 caactgcata cctcgcggag acgctcggag cgcgcgtggg cctcctgtgg tttggcgtgg
    15781 tcgtcatcgg gctgggcctg ctgctcacgg gcgtgtcggt gcgcgacacg cttgcctggg
    15841 cgcacgcgga agcggatcgc caccgcgctc ccgcagcgcc cggccaatcg cggccccgct
    15901 accccgaagg cgtgtccgcc caccccggca cctgggagat cttcaccctc atgagctggc
    15961 gcgaccgccg gctcgctgca ctcagtcaag caggcttggt cgagaaattc gtcgacgcgc
    16021 tggtgtgggt tctctatccg gtcttcctgt accagcaagg cctgagcttg ccgcagatcg
    16081 gctggatcac gggcgtttac ggattcgtct ggggcggatc gcagctcgtg accggccggc
    16141 tttcggaccg gatcgggcgc cacaaaccaa acgtctgggg catgtggatt tgcggcgcgg
    16201 gagtgctcat gatggttctg ggcagcggcc ccgtctggtg ggcgctgtcg gccggcgtca
    16261 gcggcttggg catggcactt ctctacccca atttgtccgc cgcggtggcc gacatctcgc
    16321 accccgcctg gcgcggctcc gcgatcggca tctatcggtt ctggcgcgat ctgggttatg
    16381 gagtgggtgc cctgggcctt gccctggcgg ctcagttcac gggccggctc gaagcggcgt
    16441 tctggttcgt cgggatctcg atgttgctgt ccggcgccgt actggccgca ctcggcgagg
    16501 aaacccatcc tcgcctgaat ccagcctact cggggcacct gccccagaag acgtgatgcg
    16561 cgcagacata cctcgacaag tcctgctgct ggccgcgtcg caagcactcg ccgcctgact
    16621 cgcaaccgcc ttcgcgatgc acctgacctg ctcgcgatga aaactcttga tctatatcat
    16681 agaattattc tagcgaacgt tagaatatgc gatccaacgt tccatggcct cgcccgaccc
    16741 tcggcgcggc tcatggctca tcaagacacc tggcgtggag aaaaccggga atggacgagc
    16801 caatttatct ggactacaac gccacgacgc cggtagcccc cgaagtcgtc gacgccatgc
    16861 tgccgttcct gcgacagcat ttcggcaacc cttcgagcag ccatgtgtat ggccagcggg
    16921 ccgccgcggc ggtcgccgaa gcgcgcagcc aggttgcggc gctggtcggc gcgcgcccgg
    16981 cggagatcgt cttcaccggc tgtgccaccg aggccaacaa cctggccctg ctcggcgtgg
    17041 cacgcgccct tggcgaggca aaacgccacc tggtcgtcag tgctgtggag cacccggcgg
    17101 tcatggcccc ggccgcacgg ctgcaagagc aaggctgggc cctgacagtg gtgggggtag
    17161 accagttcgg ccgcgtctcg ccggatgagg tccagcgcgc gctccgtccc gacacggcga
    17221 tggtgtcggt gatgcacgcc aacaacgagg ttggcacgat ccagccgatc caggagatcg
    17281 cacagctcac gcgagcacgc ggcatcgtgc tgcatgtcga tgcggcccag tcagccggca
    17341 agatcccggt caatgtcgat gcgctcggcg tcgatctgct gacgctcgcg gggcacaagt
    17401 tctacgcgcc aaagggcatt ggcgcccttt acgtgcgtgc cggcacgcca ttgaagtccg
    17461 ttgcattcgg cgccgaacag gaagccgggc tgcggccggg caccgaaaac gtaccgggga
    17521 tcgtgggtct cggtgccgcg gcactgctgg cccgcgtggg acttggcgag gtgaatgccc
    17581 gcctgcgcaa actgcgagat cgattgcaca ccctgctgct tgagtccata cctgggcttg
    17641 ggcttaatgg gcatcccgag catcggctac cgaataccct gcacgtgtcc ttcccgggcg
    17701 taagcgggcg cgcgctgcta ctcgaagccg ccggcatcgt ggcagcgtcg gtgggttctg
    17761 cctgccattc ggaacatgac gctgtcagcg gcgtgctggc cgccatgggc gcggacgcca
    17821 gccgcgcgag cggtgcagtg cggctctcgg tcggccggac gaccaccgaa gacgacatct
    17881 cgcgcgcagc gagcggtctg acgtcggctt ggataaggat ggccggcaga tgaaaccaat
    17941 gggcctccgt ttgtcgaacg ccctccgtac gggtcgtcga ctcaacagta atacgagttc
    18001 gtttcgtctg atgcaatgga tccaagcatg aaaaccctct tcatcctcaa cgacgcgccg
    18061 tatggaaccg agcgcagtta caacggcatg cgtcttgcgc gccatctgct caagaccggt
    18121 tccgaggaag tgaaggtctt tctgatgggc gacgccgtcg cctgtgccaa ggcgggccag
    18181 aaagtcccac aaggctatta caacgccggc gacatgctcg gcatgatcgt ccgtgccggc
    18241 ggcgagatca gtgtctgtgg cacctgcatc gatgcgcgcg gcatatcggc cgaggaactg
    18301 atcgaaggag cctcgcgcgg cggcatggac gtgctcaccc gttggagcca gtgggccgac
    18361 aaggttgtca ctttctaaat gccatgagcc gcaccgcact ggtcctgggc gccggcatcg
    18421 gcggtctggt tgccgccgaa acgctccgca agctcttgcc gccgtccgac cgcgtgatcg
    18481 ctgtcgatcg tgcggcagat catttcttgg catcctcgct cctttggttg atggtgggcc
    18541 aacgcgagcc ggcggacttc acgcgcccgc tgggccgact ggcggagcgg ggagtggatc
    18601 tgcgttgtgg caacgttacg cgcatcgacc cgggacgctg cgcggccgag atcgacggag
    18661 aagcgatctc cgctgacgcc ttggtaattg cgctcggggc cgactacgca cccgaggcga
    18721 ttccggggtt ggccgttgca ggattgaacc tgtatgcgat ggagggcgcg atcgccatcc
    18781 gcgacgcact cgcccgcttc gagggcgggc gcatcgtcat catgactgcc gccccggcgt
    18841 acaagtgccc ggccgcgcct tacgaggccg cgatgctggt tgactcctgc ttgcgcaagc
    18901 aaggcgtgcg cgacaagacg acgatgtttt tcttcgccgc tgaaccgcag ccgatggtcg
    18961 tgaccggtcc cgagattggc gccgccgtgc gcggcatgat cgaggcgcgc gggatcgtct
    19021 atcgaccgga gcaccaggtc gaggaggtca atccctcgac tcgtcaaatc aatttcacca
    19081 atggtgtcgc cgcgcgattc gacctgctca tctacgtccc gccgcaccgc gcccccgcgg
    19141 tggcgcgcaa cgcgggcctc accaacgaca gtggctggat tcccgtcgat cggcatacgc
    19201 tgcagacctc gttcgagaac gttttcgcga tcggcgacat caccagcgtc ccgctcaaaa
    19261 tggggcgccc gctgcccaag gcaggtgtgt tcgcccatgg gcaagccgag gttgtggcga
    19321 acaacattgc gcgtgcctgg accggcaagg gttcctcgcg gcaatttagc ggccaaggca
    19381 tgtgctttat cgagaccggc gatgggcgcg cgggaatcgg caagggcaat ttctacgccg
    19441 agccgacacc agaagtggaa atgcacggcc cgaatcttct ctggcatgcc ggcaaggtgc
    19501 tgtacgagaa gtactggctc taccgccgct tccgagccgg gctctagcgt ggcgaaaagc
    19561 gtccaacgat aaggcagacc atgttcaatt tttcactcgt cgatttctcg atccgccggc
    19621 caaaactgat cgtctgggcg actgtcgtgg tgaccctgct gttcctcacg cagtttgcgg
    19681 ggctcacaac cgatacgaac ccgaagcaca tgctgccgga gacttccgac gtgcgcgtct
    19741 ggaacgacga ggtggacaag accttctcgc tgtacgagga caccatcgcg gtaggagtgc
    19801 acaacgaggc aggggtgctg aaccgggaaa cgctcggccg catcgcccgc atcaccgatg
    19861 cgatcctcaa gctcgaaggg gtggcgagcc gggacgtgaa tggctttacc acgatcacca
    19921 acgtcaccgc cgagggcggc acactgaagg tggggccgct catgcccgga gcaccgaaga
    19981 ccgacgcgga ggtcgaggcg ctgcgcgccg cgctcttcgg aaatccgctg ctgcagggcc
    20041 gcgtcatctc gcgcgatggc aagacgaccg ctatttacgt gccgctggag aagggcgcca
    20101 acggcgccga catcgcgcag aaaatccggg gcgtcgtgtc cgcagagaag ggcccggagc
    20161 gctactacat cgccggggac ccggtcgcgc gcgatacgtt cggggccgaa atgttcaagc
    20221 tgatgggcat cttctcgccg attgccggca tgatcatgtt cgccgccatc cagttcatgt
    20281 tccgcagcct ggcgctctcg ttcgcgatga tggcggtggc gatggcggcg atcatctgga
    20341 gcatgggcct gctgatcggc gcgggctttc cggtgcacat catgagctcg atggcgccag
    20401 tgttcctgat ggccatcgcc accgacagca tccacatctt caacgagttc tatttccggt
    20461 atcgcgagcg gcgcgacaag ctggccgcaa tccgcgagac catgcaggcg gtcagccgcc
    20521 cggtgcgcaa cacggccctc gccacggccg ccggcttcgc cgtgctgctg ttcatggaca
    20581 ttgtgccggt gcaggtcttc ggcggcctga tcgccttcgg aacgattgtg ctgcggctgt
    20641 tctccttcag cctgctgccg gccatcctga cctttgtcag cgaagaaaag atcgctcgtg
    20701 ccgccgatca cgaaagcatg gagctcgatc gcagtgcccg cctcctgcgg cggatcgggt
    20761 ccatcggcgc cgatcacccg gggacgaccg cactggttgg cttggccctt gtcattgcag
    20821 ccatcgtcgg cacctcgaag atccacatca acaacaacat ggtcgcgtgg ttcaaggagt
    20881 cgagcgagat ccgggtggcc gacacggcgt tgaatcgcgc gctgggcggt acctccctgg
    20941 gctatctggt ggtgcagtcc acggaaccgg acttcatgaa gcggcccgag gcgatgcgct
    21001 ggctggaggg cctgcagcgc cacctcgaaa cttccccggt ggtgggcaag acgttctcgg
    21061 tggcggatta cgtcaagcgc atcaacctgg tgctgcacga taacgacgcg cagttcgccg
    21121 ccgtgccgca gacgcaggag atggtcggcc agtatctgtt cctgttcagc atgtcggcca
    21181 agccttcgga tctcgacaac gtggtggatc cgggcttccg caaagccaac gtctgggtgc
    21241 aattgaagac ctgggacgcc gacgccatgc gccaggtcat tcaatcggca caaacctacc
    21301 agaaggccaa ccccctggcg gtgacggtga agccggccgg caccgcctac ttcaacctgg
    21361 tgtggaacga cgaagtgctg ggcgacatgg tcctgggcct ggggctggca ctggtggtgg
    21421 tgttcgtcat cctggcactg gacttccgct cggcccgctg ggcgctggtg ggctacgttc
    21481 cgctgctgtt caccatcctc ctcatctacg gtgtggtcgg cttcgcgggc aaggacttcg
    21541 acatgccgct gtcggtactg agcagccttt ccctcggcat ggcggtggac ttcgcgattc
    21601 atttcatcag ccgctttcgc cagcgactgg ccgaggcggg cggcgacgtt cgtgaggcgc
    21661 tgctgtggac tgccgcgcgg cctggcaagg ggatcgtccg gaacgccttg ctgttcgccg
    21721 cggccttctc ggtcatgctg tttgcccccc tgaccccgta catcgcggtg ggtgcattca
    21781 tcgccgggat gatgctgctc tcggcgctgt tcaccatcct gtatctgccg gcactcgtca
    21841 tgctcgggcg cggctggctg ctggatcaac cgatgccggc ggcgcagccc gttccggcct
    21901 ttgcaaaagg acaatgacat gaaccgctgg tcgctatgga tactcgccag cttgctgccg
    21961 ctgcaggcac tggccttgac gggcgaggaa gtcatgagga aatcgcaggc ggcgttcttc
    22021 tacccgggca aggacttcaa ggcgcgggtg gtgatgaagc tcatcaacaa ggacgggcaa
    22081 gagcgggtgc gcgagatgac catgctgcga aagaacgccg gcgagccggg cggcgatcag
    22141 aagtatttca tgtacttctt ccagccggcc gacgtgaagg acatgagctt catgatccac
    22201 aagtatccgg ccagggacga cgaccgctgg atgttcattc cggcgatcaa catggtcaag
    22261 cgcatcgctg cgcaggacaa gcgctccagt ttcgtcgggt ccgacttcac ctacgaggat
    22321 gtctccgggc gcgatctcga ggacgacagc cacgtgctgg agcgcgagga aaaagtcggc
    22381 caacgcgagg tctacgtggt caagagcacg cccaaaggcg cggacgccga cttcggctac
    22441 aagttcacgt gggtggacaa ggccaatttc ctgccgctca aggaactgca gcatgaccgc
    22501 aaaggcgctg cctacaagca gttcagcgcc gacgaagtag ccgacatcaa gggccagccg
    22561 acggtgatca aacgcaccat gaggaacgtg cagaccggcc atcgcagcga agtcagctac
    22621 ctgaaggccg actatgacat gggcatcgag gacagcctgt tttcggagcg gttcctcagg
    22681 cagccgcctc gcaagtgggt ggattgatgc ggcatgcaat agggtgcgca gtcgcgctgc
    22741 taggggctct agcgccgctg gccgcacgcg cggacgtctc gctgtcgggg tttctgcagc
    22801 agaatacggc cgtcaacacc gcgtcggcaa accccgatgg ccgacaccag aagtggctgg
    22861 aggagcgcgc gcaactcaag cttgacgccg ccgggggcgc atggcgcctg ctggcaaagg
    22921 gtgacgttgc ctatgaccac ctgggccgac aggagcaatc cgagctgcgc gagggttata
    22981 tcgattacac agccggcaat tgggacttgc ggctgggacg gcaggtcatc acctggggtc
    23041 tgggcgacct ggtgtttgtc aacgacgttt tccccaagga ccacgaagcg cttttcgccg
    23101 gccgcccgct cgagtacctc aagcgcgggg tggacgcggt gaagttcggc ctctacccgc
    23161 agctcgcctc cttcgagctg gtcgttgcgc ctcgattccg cgagagcacc atccccgatg
    23221 cgcgccgctt tcacctgttc gatcccatgc cggcggcggc gaaccgggaa accgtcacgc
    23281 ccggcaaagg tgaggccggg ttgcgcatct atcgggatat cgccggttac gatgccgcgc
    23341 tctacctgta ccgcggcttt caccgcacgc cctcgatgcg gccggacagc gcgacggacc
    23401 ccacccagat cacctttttt cacccggggc tttcggtgta cggcgccagc gtgtccggcc
    23461 gggcgggcga aggggtggcg agcctggaaa ccgcctatta cgactcgcgc gaagaccgcg
    23521 cgggcagcga cgccgccgtt cccaattcgc aagcgaggct gcttgtcggc taccagatcc
    23581 agcccctgga ggatctctcg ctcggcttgc agtactacgg cgaatacatg cgccactacg
    23641 cggcctaccg cgcttcattg cccgccggct ttccggtcga caaacgctgg agccacacca
    23701 tcacggcgcg actgacgcag ttctttctcc accagacgct gcggctttcg gtctatgcgt
    23761 cgtataacgg cgccaacggc gaccgtttcc tgaaccccga actgcgctac agctttaccg
    23821 acaaggtgtg gggcgcggtg ggtgccaatc ttttcgatgg caagccgtgg ggacagttcg
    23881 gacagcttgc gcgggacgac aacgtgtatc ttcaggttcg ctatgagttc tgatgcttct
    23941 gcaggtgggc caatcttgta gggtgggcag cttgccgccc atcgaagcgt aggtcgcgcc
    24001 gcggtggggc aagcgcagcg gcttaggcag gctagccgcg gactgaagcg ggctcggccc
    24061 gaacgggtca gcgcggacgc aaacacatca ctcagctagc cgatttccgc gatcagctcg
    24121 atctcgacgc aggcgccggt cgggatctgc gcgacgccga aggcgctgcg ggcatgcgtg
    24181 ccctcgtcgc cgaagacctc gcccagcaat tcgctggcgc cattggtcac caggtggtgc
    24241 tcggtgaagt cgccggtcga gttgaccagg ctcatcagtt ttacgatgcg cttgaccttg
    24301 ccaaggtcgc cca
//
LOCUS       DASWIY010000265         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1275145, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000265 DASWIY010000000
VERSION     DASWIY010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275145"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VGA59_09810"
     CDS             complement(<1..364)
                     /locus_tag="VGA59_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9720014.1"
                     /translation="MRRWYWLAVPLLAAGALAFAEDRVQTDLYTVVDGTKVDQNTMEG
                     FRTWRAAACDRCHGANQEGMVGPSLLESLKVLSKADFKKAITEGRLEKGMPGFSTSQK
                     VMSNLDNLYAYLKGRSDGA"
     gene            complement(443..2245)
                     /locus_tag="VGA59_09815"
     CDS             complement(443..2245)
                     /locus_tag="VGA59_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HEX9720015.1"
                     /translation="MKITTKLLAVSVAAAGAFAGSAVANDELARLSADPKNWAMQAGD
                     FANTRFSQLKQVNKSNVNKLQVAWMFSTGVLRGHEGSPLVIGSSMYLHTPFPNNVFAI
                     DLATQKIKWKYAPKQDPAVIPQMCCDTVYRGLAYAGGKIFLQQADSMLVALDANKGTV
                     VWSVKNGDPKLGAVNTNAPHVFKDKVITGISGGEWGVRGFLAAYDMNTGQQVWKGYST
                     GPDNEMLIDPQTTMTWTDGSMQPVGADSSLKTWQGDQWKIGGGTTWGWYSYDPDLNLV
                     YYGTGNPSTWNPAQRPGDNKWSMTIFARDVDTGKAKWVYQMTPYDEWDFDGINEMILA
                     DLNVKGKYHRALVHFDRNGFGYTLDRATGELLVAEKYDPVVNWATHVDMRTGRPQVVA
                     KYSTAQNGPDVNTKGICPAALGTKDQQPAAFHPGTKLFYVPTNHVCMDYEPFEVEYVA
                     GQPYVGATLSMYPPPGSHGGMGNFIAWDAAAGKIVWSKPEKFSVWSGVLTTAGDIAFY
                     GTLEGYIKAVDISNGRELWKFRTPSGIIGNVFTYQFAGKQYVGVFSGIGGWAGIGMAA
                     GLEKDTDGLGAVGGYRELREYTDLGGQLTVFAIP"
BASE COUNT          380 a          862 c          792 g          519 t
ORIGIN      
        1 tcgcgccgtc ggagcggccc ttcagatacg catacaggtt gtccaggttg ctcatcacct
       61 tctggctggt gctgaagccg ggcatgccct tctccagccg gccttcggtg atggctttct
      121 tgaagtcggc tttgctcagc accttcaggc tctcgagcag cgagggtccc accattcctt
      181 cctgattggc gccgtgacag cggtcacacg cggctgcccg ccaggtgcgg aagccctcca
      241 tggtgttctg gtcgactttg gttccgtcca ccaccgtgta caggtcggtc tgcacccggt
      301 cctccgcgaa cgccagcgcg cccgccgcca gcagcggcac ggccagccaa taccaccttc
      361 tcatcttgat ctcgtctcca gctggaaaaa aatggcagcc cggccgaggc cgggatgcca
      421 gtgaattgct gctcgtctgc cctcagggaa tggcgaagac cgtcagctgt ccgccgaggt
      481 cggtgtactc gcgcagctct ctgtagccgc ccaccgcacc cagaccgtcg gtgtccttct
      541 ccagacctgc cgccatgccg atgccggccc agccgccgat accggagaac acgccgacgt
      601 attgcttacc ggcgaactga taggtgaaca cgttgccgat gatgccggac ggagtgcgga
      661 acttccacag ctcgcggccg ttgctgatgt ccaccgcctt gatgtagccc tccagcgtgc
      721 cgtagaaggc gatatcaccc gccgtcgtca acacaccgct ccacaccgag aacttctcgg
      781 gcttggacca cacgatcttg cccgcggccg cgtcccacgc gatgaagttg cccatcccgc
      841 cgtggctgcc cggcggcgga tacatcgaca gcgtcgcccc gacgtagggc tggccggcca
      901 cgtactcgac ctcgaacggc tcgtagtcca tgcacacgtg gttggtcggc acgtagaaca
      961 gcttggtccc ggggtggaat gccgccggct gctggtcctt ggtgcccaag gccgccgggc
     1021 agatgccctt ggtgttgacg tccggcccgt tctgcgcggt actgtacttc gcgaccactt
     1081 gcgggcggcc ggtgcgcatg tcgacgtgcg tggcccagtt caccaccggg tcgtacttct
     1141 cggccaccag cagctcgccg gtggcgcgat ccagcgtgta gccgaagccg ttgcggtcga
     1201 agtgcaccag cgcgcggtga tacttgccct tgacgttgag gtccgcgagg atcatctcgt
     1261 tgatgccgtc gaagtcccac tcgtcgtacg gggtcatctg gtacacccac ttcgccttgc
     1321 cggtgtccac gtcgcgcgcg aagatcgtca tcgaccactt gttgtcgccg ggcctttgcg
     1381 ccgggttcca cgtgctcggg ttgccggtgc cgtagtagac caggttcagg tccgggtcat
     1441 agctgtacca gccccaggtg gtgccgccgc cgatcttcca ctggtcgccc tgccaggtct
     1501 tcaggctgga atctgccccg accggctgca tgctgccgtc cgtccaggtc atggtggtct
     1561 gcgggtcgat cagcatctcg ttgtccggcc ccgtgctgta gcctttccac acctgctgcc
     1621 cggtattcat gtcataggcc gcgaggaacc cgcgcacgcc ccactcgcca ccgctgatgc
     1681 ccgtgatcac cttgtccttg aacacgtgcg gcgcgttggt gttgacggcc ccgagcttgg
     1741 gatcgccgtt cttgacgctc cagacgaccg tgcccttgtt cgcatccagc gcgaccagca
     1801 tgctgtcggc ctgctgcagg aagatcttgc cgccggcgta ggccagtccg cggtagaccg
     1861 tgtcgcagca catctgcgga atcaccgccg ggtcctgctt cggcgcgtac ttccacttga
     1921 tcttctgcgt ggccaggtcg atggcgaaca cgttgttggg gaacggcgtg tgcagataca
     1981 tgctgctgcc gatcacgagc ggcgagcctt cgtggccccg cagcacgccg gtcgagaaca
     2041 tccaggcgac ctgcagtttg ttgacgttgc tcttgttgac ctgcttgagc tggctgaagc
     2101 gggtattggc gaagtcgccc gcctgcatgg cccagttctt cgggtctgca ctcaggcgcg
     2161 ccagctcgtc gttcgccaca gccgagccgg cgaaggcgcc ggctgctgcg accgacactg
     2221 ccaacagctt ggtagtgatc ttcacgattt gctcctccta gttgtgttac ctgttctgac
     2281 tgcgaggcga tgctcgacgc tttcctgccg gatcggcggg aaacgcgctg gcgcagaatg
     2341 gaaaaaggat tcggcgccgg aacgttgatc gtgctgcctt ggctccctgt atccactggg
     2401 cggggcgcgt aaggccgcgc ccttctggcc aggctggcgc gcataaaagc cggcgcccac
     2461 gggtcggcag cttgagcccg ggtcgggccg cgtgccggga gcagaagtgt tgacggccgt
     2521 cttaccgggc cgctccaggt gttccttcgc cac
//
LOCUS       DASWIY010000266         6097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3144131, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000266 DASWIY010000000
VERSION     DASWIY010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3144131"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VGA59_09820"
     CDS             complement(<1..736)
                     /locus_tag="VGA59_09820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX9720016.1"
                     /translation="MPVITNIEDLRVLAQKRVPRMFYEYADSGSWTESTYRANEADFG
                     KILFRQRVAVNMENRTTRTRMVGQDVAMPVALAPVGLIGMQHADGEIVAARAARKFGI
                     PFTLSTMSICSIEDVAQGTGNHPFWFQLYVFRDRDFIERLIDRAKAAGCSALVVTLDL
                     QIIGQRHKDIRNGLSAPPKLTIANMLNMATKPRWGLGMLGTPRRQFGNIVGHVKGVAD
                     MGSLSEWTSRQFDPALSWKDVEWIKKR"
     gene            complement(899..1684)
                     /locus_tag="VGA59_09825"
     CDS             complement(899..1684)
                     /locus_tag="VGA59_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX9720017.1"
                     /translation="MNVFNLGLGSALFPLVVLLLILLASAIRILREYERAVVFQLGRF
                     WKVKGPGLVLLIPGIQKMVRVDLRIVTMDVPPQDVISRDNVSVKVNAVVFFRVMDPQK
                     AIIQVENFLMATSQLAQTTLRVVLGKHELDEMLAERERLNIDVQRILDAQTDGWGIKV
                     TNVEIKHIDLNESMIRAIARQAEAERERRAKVIHAEGEKQAAAALLEAARMLSQQPEA
                     MQLRYLQTMTQVAGDRASTIVFPLPMDLLGSLLGKGRATAGGG"
     gene            complement(1681..3153)
                     /locus_tag="VGA59_09830"
     CDS             complement(1681..3153)
                     /locus_tag="VGA59_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003279289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX9720018.1"
                     /translation="MGPGSTRIARHRDGAAPFWRAFVAALALCLLTISRAGAAPVVVL
                     KVQDAIGPASADYMIRGIKKAGEAGAPLVVIELDTPGGLDTSMRQLIQAILAAPVPVA
                     VYVSPEGARAASAGTYILYAAHIAAMAPATTLGAATPVAIGFPGTDRKPPGKDGDETK
                     RSGKDGDEAKGSRKPGRPASSPPAAPLGDAMTAKQVHDAAAFIRGLAQLRGRNAAWAE
                     RAVREAVTLTASEALREKVIDVVAKDLPDLLAQVDGRKVRVGAKDVQLATRGAAHEVR
                     LPDWRHQLLSVIANPSFALILMMVGIYGLLFEFSSPGFGVAGVTGAICLLLALFALQL
                     LPVNYAALALILLGMALLVAELLSPSFGILGGGGIVAFIVGGILLFDEEMPGFGIPLP
                     LVLGLAGASAAIILLGGALALRGHRRPVVSGREALVGSPGQVLEVAEGEIWAQVLGER
                     WRVTSSEPLVSGQRIRVIGLRGLTLEVRADTEPKTQGGSR"
     gene            complement(3227..4618)
                     /gene="argH"
                     /locus_tag="VGA59_09835"
     CDS             complement(3227..4618)
                     /gene="argH"
                     /locus_tag="VGA59_09835"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX9720019.1"
                     /translation="MNQLDKKSQAWSALFSEPMSELVKRYTASVYFDQRLWRVDIQGS
                     LAHADMLASQGIISRADLAAIEKGLARIVQEIESGSFEWKLDLEDVHLNIEARLTQLA
                     GDAGKRLHTGRSRNDQVATDVRLWLRGEIDLITGLLLELQKALLALAEKNVETIMPGF
                     THLQVAQPVSFGHHMLAYVEMFSRDADRMADVRKRVNQLPLGAAALAGTSYPLDRERV
                     ARTLGMEGVCQNSLDAVSDRDFAIEFTAAASLAMVHVSRLSEELVLWMSQNFGFIEIA
                     DRFTTGSSIMPQKKNPDVPELARGKTGRVVGHLMGLITLMKGQPLAYNKDNQEDKEPL
                     FDTADTLKDTLRIFAEMVGGVSTRPDAMEKAALRGYATATDLADYLVKKGLPFRDAHE
                     TVAHAVKAAVSHQVDLSELPLAVLKQFNAAIDKDVYDVLSLRGSLNAHNVLGGTAPAQ
                     VRAQIARHRARLG"
     gene            4712..5743
                     /locus_tag="VGA59_09840"
     CDS             4712..5743
                     /locus_tag="VGA59_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9720020.1"
                     /translation="MKDSQILSAFPEAPQVPSRPERALVFDACHVGVILRAVLFVEMV
                     MAAGAMFGAAGVADWTLRVAVLSGAALPATLAWLIAACSLKKLLARATPAAQQVFGIA
                     LGALAGLYGCALMALAGFVGSVPWVASAFTGALLSAALVAALVLRAKGRMPADTAARL
                     SELQARIRPHFLFNTLNSAIALVRQDPARAEAILEDLSDLFRHALMDPGQAVTLAEEV
                     ALARRYLDIEQVRFGERLQVEWAIDDSAGGARLPALLLQPLVENAVKHGVEPSPGGAQ
                     VKVSTQRRGGTVVIKVTNTVPAGQGRRGHGVAQDNVRDRLRLLHDVQCHFQTVLKDGI
                     YQVRIEVPA"
BASE COUNT          949 a         2051 c         2044 g         1053 t
ORIGIN      
        1 agcgcttctt gatccactcc acgtccttcc acgacagggc gggatcgaac tgcctggatg
       61 tccattccga cagcgacccc atgtccgcaa cgcccttgac gtggcccacg atgttgccga
      121 actggcggcg cggggtgccg agcatgccca ggccccagcg cggcttggtc gccatgttca
      181 gcatgttcgc gatcgtgagc ttgggcggcg ccgacagccc gttgcggatg tccttgtggc
      241 gctggccgat gatctgcagg tccagtgtca ccacgagcgc cgagcagccc gcggccttgg
      301 cacggtcgat cagtcgttcg atgaaatcac ggtcacggaa cacgtacagc tggaaccaga
      361 acgggtggtt gccggtgccc tgcgccacgt cctcgatcga gcagatgctc atcgtggaca
      421 gcgtgaaggg gattccaaat ttcctggcgg cgcgagcggc aacgatttcg ccgtccgcat
      481 gctgcatgcc gatcagcccc accggggcga gtgcgaccgg catggcgacg tcctggccga
      541 ccatcctggt gcgggtcgtc cggttttcca tgttcaccgc cacccgctgc cggaacagga
      601 tcttgccgaa gtccgcttcg ttggcccggt aagtgctctc ggtccacgag ccggagtcgg
      661 cgtactcgta gaacatgcgc ggcacgcgct tttgcgccag gacgcgcagg tcttctatgt
      721 tggtgatcac gggcacgggt ttgtctcctt ctcggtgccc tgaatgctag cggctcccgg
      781 ccccggcggt ttgaagaaat ttgcgcacgg ccgcaatttt gctcaaggcg atcaaaatcg
      841 cctcccgctt gttcccgccg tctgttctgg cgacaggatc ggaaaagcgg ccggtttcct
      901 acccgccccc ggcggttgcc cggcccttgc ccaagagcga tcccaacagg tccattggca
      961 gcgggaacac gatggtcgag gcgcggtcac cggccacctg agtcatggtc tggagatacc
     1021 tcagctgcat cgcttcgggc tgctgcgaca gcatgcgggc ggcctccagc agcgccgcgg
     1081 cggcctgctt ctcgccctcc gcgtgaatga ccttggcgcg ccgctcgcgc tcggcctcgg
     1141 cctggcgcgc gattgcccgg atcatggatt cgttcaggtc gatgtgcttg atttcgacat
     1201 tggtcacctt gatgccccag ccgtccgtct gcgcgtcgag gatgcgctgc acgtcgatgt
     1261 tcaatcgttc gcgctcggcc agcatttcgt ccagttcgtg cttgcccagc actacgcgca
     1321 aggtggtctg cgccagctgg ctggtcgcca tcaggaagtt ctccacctgg atgatggcct
     1381 tttgcggatc catcacgcgg aagaacacca ccgcgttcac cttgaccgag acgttgtcgc
     1441 gcgagatcac gtcctgcggc ggcacgtcca tggtgacgat gcgaagatcg acccgcacca
     1501 ttttctggat gccggggatg aggagcacca ggcccggccc cttgactttc cagaagcggc
     1561 cgagctggaa caccacggcg cgctcatact cgcgcagaat gcggatcgcg ctagccagca
     1621 ggatcagcag caacaccacc agcgggaaca gggcgctgcc caggccgagg ttgaagacgt
     1681 tcatcgggaa cctccttgag ttttcggttc ggtatcggca cgcacttcca gcgtcagacc
     1741 gcgcaagccg atgactcgga tgcgctggcc ggacaccagg ggctcgctgc tggtgacgcg
     1801 ccagcgttcg cccagcacct gcgcccatat ttcgccttcg gccacctcca gcacctggcc
     1861 cggcgagccc accagcgctt cacgaccgct gacaaccggc ctgcggtgcc ctctcagcgc
     1921 cagcgcgccc ccgagcagga tgatcgccgc cgaggcgccc gccagtccca ggaccagcgg
     1981 caacggaatg ccgaaacccg gcatctcctc gtcgaacagc agaattccgc ccacgatgaa
     2041 agcgacgatg ccgccgcccc ccaggatgcc gaaactcggc gagagcaact cggccaccag
     2101 cagcgccatg ccgagcagga ttagcgccag cgccgcatag ttcaccggca gcagctgcag
     2161 cgcgaacaag gcgagcaaca ggcagattgc gccggtcacg cccgcgaccc cgaagcccgg
     2221 agatgagaac tcgaacaaca gcccgtagat accgaccatc atcaggatca gcgcgaagct
     2281 gggattcgcg atcaccgaga gcagctggtg gcgccagtcg ggcagccgca cttcgtgggc
     2341 cgcgccgcgg gtggcaagtt gcacgtcttt cgcgccgacc cgcaccttgc gcccgtcgac
     2401 ctgggccagc aggtccggca ggtccttcgc gaccacgtca atcacttttt cccgcagggc
     2461 ctcactggcc gtgagggtga ccgcctcgcg cacggcgcgc tcggcccagg cggcgttgcg
     2521 gccgcgcagt tgcgccaggc cgcggatgaa ggcggccgcg tcgtggacct gcttggccgt
     2581 catggcatcg ccgagcggcg cggcgggcgg cgaactggcg ggccgtcccg gcttgcggct
     2641 gcccttggcc tcgtcgccat ccttgccgct gcgttttgtc tcatcgccat ccttccccgg
     2701 cggcttgcga tcggtgcccg gaaaaccgat cgccaccggc gtcgccgcgc ccagcgtcgt
     2761 ggcaggcgcc atggcggcga tgtgcgccgc atacaggatg taggtgcccg cgctggcggc
     2821 gcgcgcgcct tccgggctga cgtaaacggc gacaggcact ggcgcggcca ggattgcctg
     2881 gatcagctgg cgcatcgatg tgtccaggcc gcccggcgtg tcgagttcga tcaccaccag
     2941 cggggcgccg gcctctccgg ctttcttgat gccgcgaatc atgtagtcgg cgctcgccgg
     3001 gccgatggcg tcctgcacct tgagtacgac caccggggcg gcgccggccc gagagatcgt
     3061 caacaggcaa agcgccagcg cggcgacaaa agcgcgccag aagggggcgg cgccgtcacg
     3121 gtgccgtgcg attctcgtgc ttccagggcc catggacgca ctatgcccct tttggcggga
     3181 ctctgcctag gtcgcgccct gcgccaagcc agccagcgcc gccttgctag cccaaccgtg
     3241 cgcgatgcct ggcgatctgg gccctgacct gcgccggcgc cgtgccgccc agcacgttat
     3301 gcgcgttgag cgaaccgcgc agcgagagca cgtcgtacac gtccttgtcg atcgccgcat
     3361 tgaactgctt gagcaccgcc aggggcagct cggacaggtc gacctggtga gagaccgccg
     3421 ccttcacggc gtgggccacc gtttcgtggg catcccggaa cggcaggccc ttcttgacca
     3481 ggtagtcggc caggtcggtg gccgtggcat agcccctgag cgccgccttt tccatggcat
     3541 cgggcctggt ggaaacgccg cctaccatct cggcaaagat gcgtagcgtg tccttcaggg
     3601 tgtccgccgt gtcgaacagc ggctccttgt cttcctggtt gtccttgttg taggccagcg
     3661 gctgcccctt catcagcgtg atcaggccca tcaggtgccc gacgacgcgg ccggtcttgc
     3721 cgcgggcgag ctcggggacg tccgggtttt tcttctgcgg catgatggac gagccggtgg
     3781 taaagcggtc cgcaatctcg atgaagccga aattctggct catccacaag acgagctcct
     3841 ccgaaaggcg gctgacgtgc accatggcca gcgaggcggc ggcggtgaac tcgatcgcga
     3901 aatcgcggtc gctgacggcg tccagcgagt tctggcacac gccctccatc ccgagggtgc
     3961 gcgcaacccg ttcgcggtcc agcgggtaac tggtgccggc cagcgcggcc gcgcccagcg
     4021 gcagctggtt gacccgcttg cgcacgtcgg ccatccggtc ggcgtcccgg ctgaacatct
     4081 ccacataagc cagcatgtgg tgcccgaaac tgacaggctg tgccacctgc aggtgcgtga
     4141 acccgggcat gatggtttcg acgttcttct cggccagcgc caacagtgcc ttttgcagct
     4201 cgagcagcag gccggtgatc aggtcgatct cgccgcgcag ccacagccgc acgtcggtgg
     4261 cgacctggtc gttgcggctg cggccggtgt gcagtcgctt gccggcatcc ccggccagct
     4321 gggtcagccg cgcctcgatg ttcaagtgca cgtcttccag atcgagcttc cactcgaagc
     4381 tgccggattc gatctcctgc acgatccggg ccaggccttt ttcgatcgcc gccaggtccg
     4441 cgcgcgaaat gatgccttgc gacgccagca tgtccgcatg ggccagcgaa ccctggatgt
     4501 cgacccgcca caggcgctgg tcgaagtaca cgctggcggt gtagcgtttg accaactcgc
     4561 tcatcggctc ggagaacagg gccgaccacg cttgagattt cttatcgagt tggttcatgg
     4621 caaaggggtg gagcggggcg tttactggtt cgccccgggg gtggttgcgg caaggatcac
     4681 caataatcgg gccgaaagca accgttcctt gatgaaagat tcccaaattt tatcggcgtt
     4741 cccggaagcg ccccaagtcc cgtcgcggcc cgagcgggcg ctggtgtttg acgcctgcca
     4801 tgtcggcgtg atcctgcgcg ccgtgctatt cgtcgaaatg gtgatggccg ccggcgccat
     4861 gttcggcgcg gccggcgtgg ccgattggac gctgcgcgtc gcggtgctca gcggcgcggc
     4921 ccttccggcc accctggcat ggctgatcgc ggcctgcagc ctcaagaaac tgctggcgcg
     4981 ggcgacgccg gccgcgcaac aggtgttcgg catcgccttg ggggctttgg cgggcctgta
     5041 tggctgcgcc ctgatggccc tggcgggatt cgtcggaagc gtgccctggg tggcgtcggc
     5101 tttcacgggc gccttgctgt cggcggcgct ggtggccgca ctggtgctgc gggcgaaagg
     5161 gcggatgccc gcggacacgg cggcgcggct gtccgagctg caggcgcgca tccgccccca
     5221 tttcctgttc aacacgctca acagcgcgat cgccctggtg cgccaggatc cggcgcgggc
     5281 ggaggctatc ctcgaagacc tgagcgacct cttccggcac gcgctgatgg acccgggcca
     5341 ggcggtgacg ctggccgagg aagtggcact ggcgcgccgc tacctggaca tcgagcaggt
     5401 gcgtttcggc gagcggctcc aggtcgagtg ggcgatcgac gactccgccg gcggcgcccg
     5461 gttgccggca ctgctgttgc agccgctggt ggagaatgcc gtcaagcatg gggtggagcc
     5521 gagccccggg ggagcacagg tgaaggtaag cacccagcgc cgcggcggca ctgtcgtgat
     5581 caaggtaacc aacacggtgc cggcggggca ggggcggcgc ggccatgggg tggcgcagga
     5641 caacgtacgc gatcgcctgc gcctgttgca cgacgtccag tgccacttcc agaccgtgct
     5701 gaaggatggg atctaccagg tccgcatcga ggtaccggca tgagggcgaa gggcgaggca
     5761 gcgccggaac gtggcgcctc ggtacgtgcg cagtggaggc gctgcgacga ttcggctgcg
     5821 cggagcagct gcgcaggctc tgccgctgcg ccgaatcccc gcgtgcgggg atcgaccagc
     5881 gcgattgccg cctgccgccg acccgcgggc gctagcaccc aatgcttgtc cggaggcggc
     5941 gcgtgagctt gcaggtgttg ctggtggacg acgaggcact ggcgcgttcg cgcctgcgca
     6001 ccctgttggg cgattgcggc gccccgacgg ccgccgtcgc cggtgaggcc tccaattcgg
     6061 cccaggcgat ggagctgctg cagcggctga ctttcga
//
LOCUS       DASWIY010000267         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_551818, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000267 DASWIY010000000
VERSION     DASWIY010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551818"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1721)
                     /locus_tag="VGA59_09845"
     CDS             complement(93..1721)
                     /locus_tag="VGA59_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HEX9720021.1"
                     /translation="MNGLMMQQPLLISSLLVHAERHHPEQEIVSRRVEGDIHRETFRE
                     LAARSRRMAKAVLALGVKPGDRVGTLAWNTYRHMELYYAVSGSGAVLHTLNPRLHPDQ
                     LVWIADHAEDQVLFFDLTFLPLVEAVAPRVKTIKTFVAMTDRAHMPKESRIPNLRCYE
                     ELLQAQDDRYEWPSFDENTASSLCYTSGTTGNPKGALYSHRSTVLHTYAIALPDALNC
                     SSRDSILPVVPMFHVNAWGLPYAACMVGAKLVFPGAGLDGKSLHELFETEKVTVSAGV
                     PTVWQGLLAYVEGNDLKFSTMTRTIIGGSACPPAMIRAFQEKYGVFVLHAWGMTEMSP
                     LGTACTFKPKHLKQTQEERYVVQSKQGRGIYGVDMKIVSEDGKELPWDGQAAGDLYVR
                     GPWIVSGYFKGEGGDPLVDGWFPTGDVAKIDADGYMQITDRSKDVIKSGGEWIGSIDL
                     ENIAMAHPKVAMAACIAAHHPKWDERPLLVVMKKPGTDLTREELLAFYDGKIAKWWTP
                     DDVVFVDAIPLGATGKMQKNKLREQFKDHKLPTA"
     gene            complement(1816..>2324)
                     /locus_tag="VGA59_09850"
     CDS             complement(1816..>2324)
                     /locus_tag="VGA59_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720022.1"
                     /translation="FALPALAALLAACTSYGPGALPKGSSVAAVTQTMGPPTGEHALP
                     SGGRRLEFARGPFGRQTYMLDFDAAGLLVGSQQVLTEANFNAIQAGMSAAQVLSLIGR
                     PAKTWGISRQKQIVWSYRYDSTPFCQWFMVGMGYDDVVVDTAYGPDPLCDDDDFSIFG
                     RGIFRIGR"
BASE COUNT          359 a          753 c          796 g          416 t
ORIGIN      
        1 tcgccgagcc gaccagcagc atgtcgatcc agaacaggcg cgagagcgcc acgccgccat
       61 gccagcgaga agaaaagaag gcggtcaccc tctcaggccg tgggcagctt gtggtccttg
      121 aactgctcgc gcagtttgtt cttctgcatc ttgccggtag cgcccagggg gatggcgtcg
      181 acgaacacca cgtcgtcggg cgtccaccac ttggcgatct tgccgtcgta gaaggccagc
      241 agttcctcgc gcgtgaggtc ggtgcccggc tttttcatca ccaccagcag cgggcgttcg
      301 tcccacttgg ggtgatgcgc ggcaatgcat gccgccatcg ccaccttggg gtgggccatg
      361 gcgatgtttt ccaggtcgat ggagcctatc cattcgccgc cggacttgat gacgtctttg
      421 ctgcggtcgg tgatctgcat gtagccatcg gcatcgatct tggccacgtc gccggtgggg
      481 aaccagccgt ccaccagcgg gtcgccgccc tcacccttga agtagcccga cacgatccag
      541 gggccgcgca cgtacaggtc gccggcggcc tggccgtccc agggcagctc cttgccgtct
      601 tcgctcacga tcttcatgtc cacgccgtag atgccgcggc cctgcttgct ctgcacaacg
      661 tagcgctctt cctgcgtctg cttgaggtgc ttgggcttga aggtgcacgc ggtgcccagc
      721 gggctcatct cggtcatgcc ccaggcgtgc agcacgaaca cgccgtactt ctcctggaag
      781 gcgcggatca tggccggcgg gcaggccgag ccgccgatga tggtgcgcgt catggtgctg
      841 aacttgaggt cgttgccttc gacgtaggcg agcagcccct gccacacggt gggcacgccg
      901 gccgacacgg tgaccttttc ggtttcgaac agctcgtgca gcgacttgcc gtccagcccc
      961 gcgccgggga acaccagctt ggcgccgacc atgcacgcgg catagggcag gccccaggcg
     1021 ttgacgtgga acatgggcac caccggcagg atgctgtcgc gcgaggagca gttcagtgca
     1081 tcgggcaacg cgatggcgta ggtgtgcagc accgtcgagc ggtggctgta gagcgcgccc
     1141 ttggggttgc cggtggtgcc cgaggtgtag caaagcgatg acgccgtgtt ctcgtcaaag
     1201 ctcggccatt cgtagcggtc gtcctgcgct tgcagcagct cttcgtagca gcgcaagttg
     1261 gggatcctgc tttcctttgg catgtgcgcg cggtctgtca tcgcgacgaa ggtcttgatg
     1321 gttttcacgc gcggcgccac cgcctccacc agcggcagga aggtgaggtc gaaaaacagc
     1381 acctggtctt cggcgtggtc ggcaatccac accagttggt cggggtgcag tcgcgggttg
     1441 agcgtgtgca gcaccgcgcc gctgcccgag acggcgtagt acagctccat gtggcggtag
     1501 gtgttccagg ccagcgtgcc cacgcggtcg ccgggcttga cgcccagcgc cagcacggcc
     1561 ttggccatgc ggcgcgagcg ggcggcgagt tcgcggaagg tctcgcggtg gatgtcacct
     1621 tcgacccggc gcgacacgat ctcctgctcg gggtggtggc gctcggcgtg caccagcagc
     1681 gacgagatca aaagcggctg ctgcatcatc aatccgttca tcgcgggtcc tttcgacgag
     1741 gtgggtgctg atctggatcg gcggcaattt gcttccgcga gcttacgcgc gccgggccgt
     1801 cggccctatc gggtcttatc gcccgatgcg aaaaatgccg cgcccgaaga tcgagaagtc
     1861 atcgtcgtcg cacagcgggt cggggccgta ggcggtgtcg acgaccacgt cgtcgtagcc
     1921 catgccgacc atgaaccact ggcagaacgg cgtgctgtcg tagcggtacg accagacgat
     1981 ctgcttctgg cgcgaaatgc cccaggtctt ggccggccgg ccgatcagcg acagcacctg
     2041 cgcggcgctc atgccggcct ggatggcgtt gaagttcgct tcggtcagca cctgctgcga
     2101 gcccaccagc agcccggcgg cgtcgaagtc cagcatgtag gtctgccggc cgaagggccc
     2161 gcgcgcgaac tccaggcggc gcccgcccga gggcaaggcg tgctcgccgg tgggcgggcc
     2221 catcgtctgc gtcacggccg cgacgctgct gcctttgggc agcgcgcccg gcccgtagga
     2281 cgtgcaggcg gccagcagcg cggccagcgc cggtagggcg aagc
//
LOCUS       DASWIY010000268        18096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_910049, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000268 DASWIY010000000
VERSION     DASWIY010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 18096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18096
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910049"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VGA59_09855"
     CDS             <1..400
                     /locus_tag="VGA59_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX9720023.1"
                     /translation="LSMIGEELIVSHMDTCVLYENCAYLPEWPGVPIGDEEGRLISSA
                     LGDKQAVLLAHHGLLTAAKTVEDAAVLAFYVERAAKLQLIARAVRPIKPVKPELAREA
                     RRYRGSPKYMAATFNYLARRVLREMPDCMH"
     gene            480..1451
                     /locus_tag="VGA59_09860"
                     /pseudo
     CDS             480..1451
                     /locus_tag="VGA59_09860"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
     gene            complement(1572..2720)
                     /locus_tag="VGA59_09865"
     CDS             complement(1572..2720)
                     /locus_tag="VGA59_09865"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9720024.1"
                     /translation="MGCLNPGGLARLRSVLQSHVDRQRIPGAVAVVALGGHVELFEAL
                     GRRDPAAGTPMQGGAIFRIYSMTKPLVSLAALMLMEEGRLQLGDAVATYLPEFARQQV
                     AVDEGGSVRLEPARREATVHDLLRHTAGLTYEFLGSSAVQRQYEATQIASRSRSNAEF
                     CKALAALPLAHQPGSRWEYSRATDVLGALLEVVAAQPLGELLQERILAPLGMKDTAFA
                     VPEDSWDRIAQPFPNDPDSGEPVNMLDGREVPRFESGGGGLLSTADDYVRFLQFMRNR
                     GTLEGCRLVSRKTIDWMTSDHLGAIPIQGELLLPGYGFGLGFAVRTHAGLAPQPGSPG
                     QYFWSGIGGTSFFVDPAEDLFAMLLTQAPGQRIYYRNMFRQLVYAALD"
     gene            complement(2720..3739)
                     /locus_tag="VGA59_09870"
     CDS             complement(2720..3739)
                     /locus_tag="VGA59_09870"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX9720025.1"
                     /translation="MFATAIAGSLPKPGWLAETHKLWPQWKVQGDELRQAKADATLLW
                     IKAQEDAGLDIVGDGEQSRQHFVHGFLEQVEGIDFEHKVKMGIRDNRYDAMVPQVVAP
                     LKLKGRVHAFEAQLARAHTKRKLKFTLPGPMTIVDTVADRFYGDKVKMAFAFAELLNQ
                     EALALQADGVDIIQFDEPAFNVYMKDAADWGVKALERAAQGLACTTAVHICYGYGIKA
                     NVDWKNALGEEWRQYEQIFPALAASSIDQVSLECYHSHVPPQLMKLLAGKDVMVGVID
                     VASDEIETPEQVADTIGKALQFVARERLIACTNCGLAPMDREVALKKLEALGKGAELA
                     RRRYG"
     gene            3794..5155
                     /locus_tag="VGA59_09875"
     CDS             3794..5155
                     /locus_tag="VGA59_09875"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9720026.1"
                     /translation="MASPTQGKPRIVIIGCGFGGLEAARAFKSARVEITLVDRTNHHL
                     FQPLLYQVATAGLSAPAIAAPVRHLFRKQANVTSLLGEVKAVDTARRQVLLDGGPALA
                     YDHLIVAPGAAHSYFGHDEWARDAPGLKTLDDAFEIRRRILLAFEAAEKETDPVQRQG
                     WLTFVVIGGGPTGVEMAGQLAEIARHTLPGEFRRIDPASARILLLEGGPRVLQAMPED
                     LSRRAQEQLEKLGVEVRLDARVVAIDSAGVEVEPATAGGGTAAGRYAIATRCVVWAAG
                     VAASPLGRQLAQATGAACDRAGRVVVEPDLSVPGHPEISVIGDLAAANSHAPGRAPQP
                     VPGVSPAAKQMGRAAAANILRRMAGQPTRPFRYRNYGNLATIGRNAAVVDLMLPSGRH
                     IKFSGYPAWLFWLFAHIYFLIGFRNRIVVLIDWASAYWSQQRYARVVTDIAPHTDLQR
                     RTP"
     gene            5152..5538
                     /locus_tag="VGA59_09880"
     CDS             5152..5538
                     /locus_tag="VGA59_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX9720027.1"
                     /translation="MSIRIVRLGTPRTDGEGLRLGTVRRPPRGVPKAQFASRDFFDTW
                     LPNLSPSAPLMAEGQAAQDEKSWAAFKRKFHAEMKQPDASHLLDLLAAMSHQTTFSLG
                     CYCEDENHCHRAVLRELLAQRGAVID"
     gene            complement(5856..6101)
                     /locus_tag="VGA59_09885"
     CDS             complement(5856..6101)
                     /locus_tag="VGA59_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720028.1"
                     /translation="MRILTSMLTALFLLMAGTAWANQCPAMMKAIDDKLATKPTLSKD
                     VSDKVVKLRKDGEAAHKAGKHADSEKALGEAKKLLGI"
     gene            complement(6172..6525)
                     /locus_tag="VGA59_09890"
     CDS             complement(6172..6525)
                     /locus_tag="VGA59_09890"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX9720029.1"
                     /translation="MGPAGNRLAPAPVLYVVEGPEHGFSDMPTAVYWAITTVGFGDIT
                     PKTDIGRLIASVMMLLGWGTLAVPTGIVTAEMTVRRHARPLPVARACTQCGAEGYGPE
                     AKFCQQCGAELPGPL"
     gene            complement(6553..7668)
                     /locus_tag="VGA59_09895"
     CDS             complement(6553..7668)
                     /locus_tag="VGA59_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX9720030.1"
                     /translation="MLKISKVEPFVLHVPVTGSQIADSTHSITHWGVVGARIDTEEGL
                     SGFGFTGTHAWLPGDQLIARCIATCHAPLLIGEDAGDVQRLWLKLARNPALQWIGRAG
                     ITTLSQAAIDVALWDLKAKQAGVPLWKLLGGQVRDKVRAYNTDIGWLSIDDDRLMQGA
                     LRAVGEGFTGIKIKVGSTVERDLRRLEAVRRAIGPDITLAIDGNGKWDLPTCLRFCRA
                     ADAFDVYWFEEPLWYDDVKGHAELARATSIPVALGEQLYTQDAFSEFFHQGAVHWVQP
                     DVTRMAGLTEVWRVCETAHSHRLPVAPHAGDMSQVHVHLSYAHPACAILEYIPWIKDC
                     FTEPADVVEGNFRLPQQPGAGTTPTREAWEKFRQSAA"
     gene            complement(7783..8778)
                     /locus_tag="VGA59_09900"
     CDS             complement(7783..8778)
                     /locus_tag="VGA59_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX9720031.1"
                     /translation="MSSVPVPSTDWKEKIAPGEEQRYAGYARQFAQIQARKSARWGKG
                     RTLHRKQITAAHGTLEVLDGLPGYARHGLFAKPGDHDVWVRLSNGGVNVAADKTPDIR
                     GFALRVFGVQGKSALGNGPAVSQDFTLINQEVFAFHNSDEFVAFVVAAARGNREMFRH
                     IFKRYGLLGGPRQLGKLLKVSGKPFSGFATEPLFSTVPIANGPYAVRVRLLPAASNGT
                     PAAGAREDWAADFCSRLARHPLHWDLQLQFFASEALTPIEDACVNWSTPYSTVARLML
                     PKQDCASAEGQALAEQVEGTAIDPWQALAEHRPLGDVQRARKVVYFESQKGRGAA"
     gene            complement(8817..10505)
                     /locus_tag="VGA59_09905"
     CDS             complement(8817..10505)
                     /locus_tag="VGA59_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9720032.1"
                     /translation="MQRPHHKFWPKRLPYALTPPATSLWDNLEISARRFPDKTALVFF
                     GRCFSYNELRLKAERLAAQLHTLGVRRGDRVMLDMQNCPQLVIAHFAILRANAVVVPV
                     NPMNRFEELKHYIEDPDAKVAITTADLAPEIARASDALPHGQRLGHLIVSHFTDAFDP
                     DAAGADAMPDAWRDWLLARHPLPALAGGQVTSWQAALDNTAPLPPLEVGPDDLAVLPY
                     TSGTTGLPKGCMHRHSSIMHNAVASSVWGNATAENVTLLVVPMFHVTGMISTMHANIY
                     TAATLVMMPRWDRELAGRLISRWKVTTWTNIPTMVIDLLASPNFDQYDLSSLVHIGGG
                     GAAMPQAVAQRLQDQFGLRYVEGYGLTETAAPSHSNPPDNPKQQCLGIPFMSCDARVV
                     DPETLQELPAGEQGEIIMRGPMVFAGYWKRPEATAAAFIEFEGKQFFRSGDLGRMDED
                     GYFFLTDRLKRMINASGFKVWPAEVEALMFRHPAIQEACVISAKDSYRGETVKAVVVL
                     RAGHKGQVSEQDIIDWCRENMAVYKVPRIVQFADALPKSGSGKVMWRTLQEAEA"
     gene            10680..11117
                     /locus_tag="VGA59_09910"
     CDS             10680..11117
                     /locus_tag="VGA59_09910"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9720033.1"
                     /translation="MIEKKASVHWEGAGTTGRGQVSTETGALQGFPYGVASRFADDRK
                     GTNPEEIIGAAHAACFTMFFAVTCEKAGITVGTIDTTATVRLAKDGEGFKIDRIALVM
                     EATVPGIDYAKFQEIAEISKKNCPVSKALAAVPEITLEAKLRT"
     gene            11221..11988
                     /locus_tag="VGA59_09915"
     CDS             11221..11988
                     /locus_tag="VGA59_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9720034.1"
                     /translation="MTSLATSSFDSAAVARRLLRAHKGAAAVPHAEVIGPDAAAAYAV
                     QDATVRGLGPVGGWKVGAKGPSAEPACAPLPAGGLFPSGTTLLGPPWRMRGIELEVAV
                     RLRRDLVPGPGEPEAAMLRDAIEAVLPVIEVVETRLADWRDSPLLAQLADLQSHGGLV
                     LGAARALSPAELDLRTVEAYLAFDGQPVASTRGANPAGDIWRLLGWLARHCAQRGQPL
                     RAGQVVTTGSCTGMLFAPEGAHVQAQLAGIGLVELRF"
     gene            complement(12062..12547)
                     /locus_tag="VGA59_09920"
     CDS             complement(12062..12547)
                     /locus_tag="VGA59_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX9720035.1"
                     /translation="MSSLTGGCDCGAIRYRMETRPLFVHCCHCRWCQRETGSAFALNA
                     MIEADRVVLLGAEPEIVDTPSASGKGQRIARCPKCRIAVWSNYSGAGPSVRFVRVGTL
                     DDPDQLPPDIHIFTASKQPWVVLPPGTPAVPEYYHRSQYWPAESLARREALLAKIAAQ
                     R"
     gene            complement(12596..13018)
                     /locus_tag="VGA59_09925"
     CDS             complement(12596..13018)
                     /locus_tag="VGA59_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9720036.1"
                     /translation="MPEVARKNAGGKAEVRVFLVEDLKSTQGLYADLFAALGGFRIVA
                     TVRTEAEANLWLEENPNGWDLAVIDLVLEQGTGMGVIPRAKALSTAGRVVVLSSYATP
                     GVRKHCLHLGADAVLDKSEASEFIRYCAEHAQRENSGP"
     gene            13236..14207
                     /locus_tag="VGA59_09930"
     CDS             13236..14207
                     /locus_tag="VGA59_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX9720037.1"
                     /translation="MNLRQVFAPLIFLVTAWCVPAAAFDLQGHRGARGLAPENTLAAF
                     ERALEVGVTTLELDIGLTADGVVVIAHDPHLNPALTRDGSGRWLAGAKGPLLRSLTMA
                     QLQAYDVGRIDPGTSYAGTFASQQPRDGMHIPSLAALFDRVKALGANDVRFNIETKIN
                     ANQPQDTVGPEAMVGALMQVVREAGMTARVSIQSFDWRTLRLVRQLEPSIPTVCLTIQ
                     TAKNDNLRDGDWTGGLKFADHGSAPKMAKAAGCAVWSPNGGAVTEPLVRQGRALGLKV
                     VPWTINDPADMERLIGWGVDGLITDYPDRLRGVMQARNLALPKALAN"
     gene            14269..14475
                     /locus_tag="VGA59_09935"
     CDS             14269..14475
                     /locus_tag="VGA59_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEX9720038.1"
                     /translation="MSNHIYKSLELTGSSTVGIEDAVSRAIAKAHETVRNIHWFNVIE
                     TRGHVEDGKVAHWQVTLKVGFTLE"
     gene            14506..15654
                     /locus_tag="VGA59_09940"
     CDS             14506..15654
                     /locus_tag="VGA59_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9720039.1"
                     /translation="MPFILYLFALTNLVIGTGAFVLSGILQPLADSLEVSVAAAGQVM
                     TAYALAAALLAPLFLLATGHWPRKRAILLALALFAAGCVLCASAGSLAVLLLGRALMG
                     VGSVFTALAAGVAVAVVAPERRGRALSLTFLGMSLSYAVGVPLGAWLGFTYGWRAPVW
                     LVAAACVAATAVLMALLPRRIEAPGARFPGLGAAARQWPILRVWLRTLLFFIAIFSVF
                     AYVGPVLLALNPLTPAQLSFTLVMFGLSGVAGTIIGGLAVDRFGPVLTIGVQIALLAS
                     MMLLVPLTRGHHALTVLVFVVWGMAGFGLTAPQQVLLVARSPQQAPLLISLNGSMLYV
                     GTALGAAVSGAFAASLGFDKLSWIGLPFALLALATLWFDTQGRMHPAH"
     gene            complement(15779..16837)
                     /locus_tag="VGA59_09945"
     CDS             complement(15779..16837)
                     /locus_tag="VGA59_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9720040.1"
                     /translation="MTSGGGLPRGVLASLSAALLFGAGTPLAKLLLAQASPWLLAALL
                     YLGSGLGLALLRRIRRAGRVRLPAGEAKWLAGAIVAGGVAGPVLLMLGLAGMPASGAS
                     LLLNAEAVFTALLAWFVFKENFDRRVALGMLAIVAGGLVLAWPGQMTLPQWQSALLVT
                     GACLAWAIDNNLTRKVSLADASWIAMVKGLAAGATNLVLALALGATWPAPAVVAAAAL
                     VGFASYGASLALFVVGLRELGTARTGAYFSVAPFFGAVLAIVLLGEAVTAPLLAAGLL
                     MAVGVALHLSERHEHRHAHEAIEHTHEHVHGAGDVHHDHTHQPPVEPGTRHSHPHRHQ
                     PMVHSHPHFPDAHHRHGH"
     gene            complement(16834..17271)
                     /locus_tag="VGA59_09950"
     CDS             complement(16834..17271)
                     /locus_tag="VGA59_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HEX9720041.1"
                     /translation="MKIGDLSRATGVEVETIRYYEKAGLLPAPAREANGYRSYGAPHL
                     ERLAFVRHCRALDMPLADVKRLLDFVGGARDDCGDIDALIDAQLLRVRARLASLRALE
                     RQLAALRDSCDADHATHECGILHELVAAAHGEACACHTDGTGQ"
     gene            complement(17276..17410)
                     /locus_tag="VGA59_09955"
     CDS             complement(17276..17410)
                     /locus_tag="VGA59_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720042.1"
                     /translation="MEFAWNRNRIGFGGQFELLDHVAEQHRTPSGTAQQFVYCHCLLH
                     "
     gene            17498..>18096
                     /locus_tag="VGA59_09960"
     CDS             17498..>18096
                     /locus_tag="VGA59_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX9720043.1"
                     /translation="MSAHCQHCQPQHDDPHRRPAPGYRRVLWIALLINASMVGVEILA
                     GLSSGSVSLLADAIDFFGDAANYGVTLAVLNLGLAWRARAALLKSASMLAFGGIVLAK
                     ALWAMSQGVPPEALTMGLVGAAALAANLGVAALLYAFRSGDANMRGVWLCTRNDALGN
                     LAVMLAALGVFRTGTAWPDLVVAIVMAGLAISSGLSVMR"
BASE COUNT         2869 a         6171 c         6044 g         3012 t
ORIGIN      
        1 gctgtcgatg atcggggagg aactgatcgt ctcgcacatg gacacctgcg tgctgtacga
       61 gaactgcgcc tatctccctg agtggccggg cgtgccgatc ggcgacgagg agggcaggct
      121 gatttcgagc gcgctgggag acaagcaggc ggtactgctg gcgcaccacg gcctgctcac
      181 cgcggccaag accgtcgagg acgcggccgt gctcgcgttc tacgtggaac gcgcggcgaa
      241 gctgcagctc atcgcgcggg cggtgcggcc gatcaagccg gtgaagccgg aactcgcgcg
      301 tgaggcccgt cgctatcgcg gcagtcccaa gtacatggct gccacgttca attatctggc
      361 gcgccgcgtg ctgcgcgaaa tgccggactg catgcactga cgccgcgagg caatcgtatc
      421 cacccctctg aacaaaccgc gacgccccga tccgagttga ttccaaccaa ggaacgatca
      481 tgacattcct tttcatacgc gcaattttcg cggcactgct cgcgcttgca gccgcgacag
      541 tctcggccca aacctacccg aacagggcga tccgcatcat cgtgccgtac ccggcaggcg
      601 gtggtgccga cattaccgcg cggccgatcg cgcagatgct cagcgaacgc tggggccagc
      661 ccgtcatcat cgagaatcac gccggtgcga gcggcatgat aggcgccgaa atcgtggcca
      721 aggcgccgcc cgacggctac actatcatgg tcagtgcctc gggggaagtc gccctcaacg
      781 taccgctctt ctcgaaaatg ccgtacgacc cggtcaagga tttcgcgccg atcaccctgg
      841 cgacggtgac gccgatggcg ctcgtcgtgc atccgtcggc gcccatcact tcgctgaagg
      901 agtacatcga gtttgcgaaa tcgaagtccg gagccatgac cttcggttcg acgggcatcg
      961 gctcggttca gcacttcgcc ggcgaattgc tgaagatgct gaccacggcc gatatcgtgc
     1021 acgtgcccta caagggctcc ggtccgttga tcaccgacct gctcggcggg cacgcaccca
     1081 gcggatttgc cacgctgctg ccggcgatgc agcacatgaa gacgggcaag ctgcgcgtcc
     1141 tggcggtgac gacgcaatag cgcgcgccgc tgctgcctga cgtgccggcc atcgcggaga
     1201 cgctgcccgg gttcgacgtc acccagtgga atgccgtgtg ggcgcccgcc gcgacgccca
     1261 aggatgtgct cgacaagctg agcgcggaaa tcaggcggat cgtccagtcg gccgactaca
     1321 aagcgcgcat gctggagcag ggcagcgaag ccgtcggcaa ttcgccagcg gaccttgcgg
     1381 cgttccagaa gaccgaaatc gacaagtacc gcaagatcgc gctgcaggcg aatatcaagg
     1441 ccaaccagtg aatttcacct ccgatcgagc gggtcggcat gcgcggtgcc ctcgtgggca
     1501 cccacgcgat aacgctctct ccctgtgggg tgggcacttg ctgcccaccg tgttccaatc
     1561 gccacccgca atcagtccag cgccgcgtaa accagctggc ggaacatgtt gcggtagtag
     1621 atccgctggc ccggcgcctg ggtcaacagc atggcgaaca ggtcttccgc gggatcgacg
     1681 aaaaacgaag tgccgcctat accactccag aaatactgtc ccggcgatcc cggctgcggc
     1741 gcgaggccgg cgtgcgtgcg cacggcgaag ccaaggccga agccataacc gggcagcagc
     1801 agttcgccct ggatcggaat ggcgcccaga tgatcggacg tcatccagtc gatggtcttg
     1861 cgcgacacca gccggcagcc ctcgagcgtc ccgcggttgc gcatgaactg caggaagcgc
     1921 acgtaatcat ccgccgtcga caacaagccg ccgccacccg attcgaaccg cggcacttcg
     1981 cggccgtcca gcatgttgac cggctcgcca ctgtcgggat cattggggaa cggctgggcg
     2041 atgcggtccc agctgtcttc aggcaccgcg aacgccgtgt ctttcatgcc cagcggtgcc
     2101 aggatgcgct cttgcagcag ctcgcccagc ggctgagcgg ccaccacctc gagcagcgcg
     2161 cccagcacgt cggtcgcgcg gctgtactcc cagcggctgc ccggctggtg cgccagcggc
     2221 agcgccgcca gggccttgca gaattccgcg ttgctgcgcg agcggcttgc aatctgggtc
     2281 gcttcgtact ggcgctgcac ggcactgctg ccgaggaatt cgtaggtcag ccccgccgtg
     2341 tggcgcaaca ggtcgtgcac ggttgcttcg cggcgggccg gctccagccg cacggacccc
     2401 ccctcgtcga ccgcgacctg ctggcgggcg aattccggca ggtacgtggc caccgcatcg
     2461 cccagttgca gccggccttc ctccatcagc atgagcgcgg ccagcgatac cagcggcttg
     2521 gtcatggaat agatgcggaa gatcgcgccg ccctgcatcg gtgtaccggc ggcgggatcc
     2581 cgccgcccaa gtgcctcgaa cagctcgaca tgcccgccca gcgcgaccac ggcgacagcg
     2641 ccgggaatgc gctgccggtc gacatgcgat tgcagcaccg agcgcaagcg tgcgaggcca
     2701 ccagggttca agcagcccat cagccgtacc tccgccgcgc cagttccgcg cccttgccca
     2761 gcgcctccag tttcttcagg gcgacctcgc gatccatggg cgccaggccg cagttggtgc
     2821 aggcaatcag gcgctccctg gccacgaact gcagcgcctt gccgatggtg tcggccacct
     2881 gctcgggtgt ttcgatctcg tcgctggcga cgtcgatcac gcccaccatc acgtccttgc
     2941 ctgcaagcag cttcatcagc tgcggcggca catgggagtg atagcactcc aggctgacct
     3001 ggtcgatcga gctggcggcc agcgccggga agatctgctc gtactggcgc cattcctcgc
     3061 ccagtgcatt cttccagtcc acgttggcct tgatgccgta gccgtagcag atgtgcacgg
     3121 cggtggtgca ggcgaggccc tgggccgcgc gctccagcgc cttcacgccc cagtcggccg
     3181 cgtctttcat gtagacgttg aacgcgggct cgtcgaactg gatgatgtcc accccgtccg
     3241 cctgcaaggc cagggcttcc tggttgagca gctcggcaaa ggcaaatgcc atcttgacct
     3301 tgtcgccata gaagcggtcg gccaccgtgt ccacgatggt catcgggccg ggcaatgtga
     3361 acttgagctt gcgcttggtg tgggcccgtg ccagctgcgc ctcgaaggcg tgcacgcggc
     3421 ccttgagttt cagcggcgcg accacctgcg gcaccatggc gtcgtagcgg ttgtcgcgga
     3481 tgcccatctt caccttgtgc tcgaagtcga tgccctcgac ctgctcgagg aaaccatgca
     3541 cgaagtgctg gcgcgattgc tcgccgtcgc cgacgatgtc caggcccgcg tcctcctgcg
     3601 ccttgatcca cagcagcgtg gcgtcggcct tggcctggcg cagctcgtcc ccctggacct
     3661 tccattgcgg ccagagcttg tgggtttcgg ccagccagcc cggcttgggc aggctgcctg
     3721 cgatggcggt cgcaaacatg aaagctccct tgacttcaaa gcgttcattg tgcctcgctc
     3781 gcataatcct ccgatggcct ctccgactca gggcaaaccc cggatcgtca tcatcggctg
     3841 cggcttcggc ggcctggaag ccgcccgcgc gttcaagagt gcgcgggtgg aaatcaccct
     3901 ggtggacagg accaatcacc acctgttcca gccgctgctg taccaggtcg ccacggccgg
     3961 gctgtcggca cccgccatcg cggcgccggt gcgccatctg ttccgcaagc aggccaacgt
     4021 cacgagcctg ctgggcgaag tcaaggccgt cgacacggcg cgccgccagg tgctgctgga
     4081 cggcggtccg gccctcgcct acgaccatct catcgtcgcg cccggcgccg cgcacagcta
     4141 cttcgggcac gacgagtggg cgcgcgatgc ccccggactc aagacgcttg acgatgcgtt
     4201 cgagattcgc cgccgcatcc tgctcgcgtt cgaggctgcc gagaaggaaa ccgaccccgt
     4261 ccagcggcag ggctggctga cattcgtcgt catcgggggc gggcccacgg gcgtggagat
     4321 ggccggacag ctcgcggaaa tcgcgcgcca cacgctgccc ggcgagttcc gccgcatcga
     4381 cccggccagc gcgcgcatcc tgctgctcga aggcggcccg cgcgtgctgc aggccatgcc
     4441 ggaagacctg agccgccgcg cccaggaaca gctggagaag ctgggcgtcg aggtgcgcct
     4501 ggatgcgcgc gtggtcgcca tcgacagcgc cggcgtcgag gtcgagccgg cgacggcagg
     4561 gggcgggacc gcggccgggc gctacgccat cgccacccgc tgcgtggtct gggccgccgg
     4621 cgtggcggcg tcaccgctgg gccggcagct ggcacaggcc acgggcgcgg cttgcgaccg
     4681 cgcgggccgc gttgtggtgg agccggacct cagtgtgccc ggccacccgg agatcagcgt
     4741 catcggcgac ctcgccgccg ccaacagcca tgccccggga cgcgcgccgc agccggtgcc
     4801 cggcgtttcg ccggccgcca agcagatggg ccgggccgcg gcggccaaca tcctgcggcg
     4861 catggccggc cagcccacgc gccccttccg ctaccgcaac tacggcaacc tggcgaccat
     4921 aggccgcaac gccgccgtgg tcgacctgat gctgccttcg gggcggcaca tcaagttcag
     4981 cggctatccg gcgtggctgt tctggctgtt cgcccacatc tacttcctga tcggtttccg
     5041 caaccgcatc gtggtgctga tcgactgggc ttcggcgtac tggagccagc aacgctatgc
     5101 gcgcgtggtg acggacatcg cgccccacac cgatttacaa aggagaacac catgagcatc
     5161 cgcatcgtgc ggctggggac gccgcgcacc gacggcgaag gcctgcgcct gggcaccgtg
     5221 cgcaggccgc cgcgcggcgt accgaaggcg cagttcgcca gccgcgactt cttcgatacc
     5281 tggctgccga atctgtcgcc cagcgcgccg ctcatggccg aaggccaggc ggcgcaggac
     5341 gagaaatcat gggccgcgtt caagcgcaag ttccacgcgg aaatgaagca gccggatgct
     5401 agccacctgc tcgacctgct ggcggcgatg tcgcaccaga ctaccttttc gctgggctgc
     5461 tattgcgagg acgagaacca ttgccaccgc gcagtgctgc gcgagttgct cgcacagcgc
     5521 ggagcagtca ttgactgaag cctcggcatg tcattgcggg ctgggctcgc ggaacgcccg
     5581 gggcgcgcag ctgtccagtg cagcgagccc aatgccgggc ctacgctatg cacgtgccga
     5641 ggcatcacgc tccggcccgg ccccgcaatc caggaagtcg tggatcccgg gtcaagcccg
     5701 ggatgacagc aatagctttg tcattgcggg cttgacctgc acaggcttgt cattgcgggc
     5761 ctgacctggc ctgacccgca atccaggaag tcctggatcc cgggtcgagc ctgccccgga
     5821 cttgatccgg ggcccgggat gacagcctat tccggtcaga tcccgagcag tttcttggcc
     5881 tcgcccagtg ccttttcgga atcggcgtgc ttgcccgcct tgtgggccgc ctcgccgtcc
     5941 ttgcgcaact tgacgacctt gtcggaaacg tctttggaaa gcgtcggctt ggtggccagc
     6001 ttgtcgtcga tcgccttcat catggcggga cattggttgg cccaggccgt gccggccatc
     6061 aacaggaaca gggccgtcag catggaagtc agtatgcgca tggaggtctc cagtcggttg
     6121 aaagaaccgc gacattagcg caaaaccggc tgggtcgttg ttttcgcctg gttacaacgg
     6181 ccccggcagt tcagctccgc actgctggca gaacttggct tccggcccat acccttcggc
     6241 gccgcactgc gtgcaggcgc gcgccacggg cagcggccgg gcgtgccggc gcaccgtcat
     6301 ctcggcagtg acgatgccgg tgggcacggc cagggtgccc cagcccagca gcatcatgac
     6361 cgacgcaatc aggcggccga tgtcggtttt cggtgtgata tcgccgaagc ccacggtcgt
     6421 gatggcccag tagaccgccg tgggcatgtc gctgaacccg tgttccgggc cttccaccac
     6481 gtaaagtaca ggcgcaggcg ccagccggtt gccggccggc ccaattggtc ttgttcggtg
     6541 gtggccatgg cctcaggccg cgctctggcg gaatttctcc caggcttcgc gcgtgggcgt
     6601 ggttcccgca cccggctgct gcggtagccg gaagttgccc tcgaccacgt ccgcgggctc
     6661 ggtgaagcag tccttgatcc aggggatgta ttcgaggatc gcgcaggccg gatgcgcgta
     6721 gctcaggtgc acatgcacct ggctcatgtc gcccgcgtgc ggcgccaccg gcagccggtg
     6781 cgaatgcgcc gtctcgcaca cgcgccagac ctccgtgagg ccggccatgc gcgtcacgtc
     6841 gggctggacc cagtgcacgg cgccctggtg gaagaactcc gagaaggcgt cctgcgtata
     6901 gagctgttca cccagcgcca ccgggatact cgtggcgcgc gccagttcgg cgtggccttt
     6961 cacgtcgtcg taccacagcg gctcctcgaa ccagtacacg tcgaaggcat cggccgcgcg
     7021 gcaaaagcgc aggcaggtgg gcaggtccca tttgccgttg ccgtcgatcg ccagcgtgat
     7081 gtcggggccg atcgcgcgcc gcaccgcctc cagccggcgc aggtcgcgct ccaccgtgga
     7141 gccgaccttg atcttgatcc cggtgaagcc ctcgcctacc gcgcgcaggg cgccttgcat
     7201 gagccggtcg tcatcgatgc tgagccagcc aatgtccgtg ttgtaggccc tcaccttgtc
     7261 ccgcacctgg ccgcccaaca atttccacaa cggcacgccc gcctgtttgg ccttgaggtc
     7321 ccacagcgcc acgtcgatcg cggcttggga cagggtggtg atgcctgccc gcccaatcca
     7381 ttgcagcgcg ggattgcgcg cgagcttgag ccacagccgc tgcacgtcgc cagcgtcctc
     7441 ccctatgagc agcggcgcat ggcaggtcgc gatgcagcgc gcgatgagct ggtcgcccgg
     7501 cagccaggca tgcgtgcccg tgaagccgaa accggacagg ccctcctccg tgtcgatgcg
     7561 cgcgccgacc acaccccagt gcgtgatgct gtgggtgctg tcggcgatct gcgagccggt
     7621 gacgggcaca tgcaggacga agggttcgac cttactgatt ttcaacatgg tttttcaatg
     7681 gcgggcaaaa ggcaatggta gcccgcgccg tgcggcctgg cagccggttg tggtggcggc
     7741 aatgccggcc tggcaaaaga ggggcatggc cgccccccga gttcaggccg cgccccgccc
     7801 cttctggctt tcgaagtaga cgaccttgcg ggcgcgctgc acgtcgccca gggggcggtg
     7861 ctcggccagc gcctgccacg gatcgatggc cgtgccctcg acctgttcgg ccaaggcctg
     7921 gccttcggcc gaggcgcagt cctgtttcgg cagcatcagc cgcgccaccg tgctgtaggg
     7981 ggtggaccag ttgacgcaag cgtcctcgat cggcgtgagc gcctcgctcg cgaagaactg
     8041 caattgcagg tcccagtgca gcgggtggcg tgccagccgc gagcagaagt ccgcggccca
     8101 gtcctcgcgg gccccggcgg ccggggtgcc gttcgacgcg gccggcagca accgcacccg
     8161 caccgcatac ggcccgttcg cgatcggcac ggtgctgaac aacggttcgg tcgcaaaccc
     8221 gctgaacggc ttgccgctga ccttcagcaa cttccccagc tggcgtggcc cgcccagcaa
     8281 tccatagcgc ttgaagatat gcctgaacat ctcgcggttg ccccgggctg ccgcgaccac
     8341 aaaagcaacg aactcatcgc tgttgtggaa ggcgaacacc tcctggttga tcagcgtgaa
     8401 gtcctggctc accgccgggc cattgccgag cgcggacttt ccctggacgc caaaaacccg
     8461 caacgcgaaa ccccggatgt ccggcgtttt gtccgcggcc acgttcacgc cgccgttgga
     8521 aaggcgtacc cacacatcgt ggtcgccggg cttggcgaac agcccgtgcc gggcgtaacc
     8581 gggcaatccg tccaggacct cgagcgtgcc gtgcgccgcc gtgatctgct tgcggtgcaa
     8641 tgtccggccc ttgccccagc gcgcggactt cctggcctgg atctgcgcga actgccgcgc
     8701 gtaacccgcg tagcgctgct cttcgccggg agcgatcttt tccttccagt cggtgctggg
     8761 aacaggcaca ctgctcatcg gtggaccctt cgccgttgtc ttgccgggaa gagagtctac
     8821 gcctccgcct cctgcagcgt gcgccacatc accttgccgc tgccgctctt gggcagggca
     8881 tcggcaaact gcacgatccg cggcaccttg tacaccgcca tgttctcgcg gcaccagtcg
     8941 atgatgtcct gttcgctcac ctgccccttg tggccggcgc gcagcacaac caccgccttc
     9001 actgtttcgc cgcggtagct gtccttcgcg gagatcacgc aggcctcctg gatcgcgggg
     9061 tggcgaaaca tcagcgcctc gacttcggcc ggccagacct tgaagccgga cgcgttgatc
     9121 atccgcttca ggcgatcggt caagaagaag tagccgtcct cgtccatacg gcccagatcg
     9181 ccggagcgaa agaattgctt tccttcgaac tcgatgaaag ccgccgctgt cgcttcgggc
     9241 cgcttccagt agccggcaaa caccatcggg ccgcgcatga tgatctcccc ctgctctccc
     9301 gccggaagtt cctggagcgt ctcgggatcg accacgcgcg cgtcgcagct catgaaggga
     9361 atgcccaggc actgctgctt gggattgtcg ggcgggttgc tgtgcgaagg tgccgccgtt
     9421 tcggtcagcc cgtaaccttc gacatagcgc aggccgaact ggtcctgcag acgttgcgcg
     9481 acggcctgcg gcatggcggc gccgccgccg ccgatgtgga ccaggctgga caagtcgtac
     9541 tgatcgaagt tggggctggc cagcaggtcg atcaccatgg tcgggatgtt ggtccaggtc
     9601 gtcaccttcc agcgtgagat cagccggccc gccagctcgc ggtcccagcg cggcatcatg
     9661 accagcgtgg ctgccgtgta gatgttggcg tgcatcgtgc tgatcatgcc ggtgacgtgg
     9721 aacatgggca cgaccagcag tgtcacgttc tcggccgtgg cattgcccca gactgagctg
     9781 gcgacggcgt tgtgcatgat gctggagtgc cggtgcatgc agcccttggg cagcccggtg
     9841 gtgccgctgg tgtaaggcag taccgccagg tcgtccggac ccacctccag cggcggcagc
     9901 ggcgcagtgt tgtcgagcgc ggcctgccag ctcgtcacct ggccgccggc cagcgccggc
     9961 agcgggtgcc gggcgagcag ccagtcgcgc cacgcatcgg gcatggcgtc ggcgccggcc
    10021 gcgtcggggt cgaaggcgtc cgtgaaatgg ctgacgatca ggtggcccag ccgttgccca
    10081 tgcggcaagg cgtcgctggc cctggcgatc tccggcgcca ggtcggcggt ggtgatcgcg
    10141 accttcgcat cgggatcctc gatgtaatgc ttgagctcct cgaagcggtt catcgggttg
    10201 accgggacca cgacggcgtt ggcgcgcagg atcgcgaaat gggcgatcac cagctggggg
    10261 cagttctgca tgtcgagcat gacacggtcg cccctgcgca cgcccagcgt atgcagttgg
    10321 gctgcaaggc gctcggcctt cagccgcagc tcgttgtagc tgaagcagcg gccgaagaac
    10381 accagggcgg tcttgtccgg gaagcggcgc gcgctgatct cgaggttgtc ccacagcgag
    10441 gtcgcgggcg gtgtgagtgc atagggcagc cgcttgggcc agaacttgtg atggggacgc
    10501 tgcattgaaa ctcctgacga gacggcgagc ctagcgggcg cggtgccgaa tggcatcagg
    10561 gatggcccca ggacggtcgc tttggcgacg cgcgcgcgcc tcgcactggc ggcgccgtcc
    10621 tacagactgc aattgcggcc ttgcgcaaac tcgggcgctc ccccacacag gagtgaacca
    10681 tgatcgagaa aaaggcaagc gtccactggg aaggcgccgg cacgaccggc cggggccagg
    10741 tcagcaccga aaccggtgca ctgcagggct tcccctatgg cgtcgccagc cgcttcgcgg
    10801 acgaccgcaa aggcaccaac cccgaggaga tcatcggcgc cgcccacgcg gcctgtttca
    10861 cgatgttctt tgccgtcacc tgcgaaaagg ccggcatcac cgtgggcacc atcgacacca
    10921 cggccacggt gcgcctcgcc aaggacggcg agggcttcaa gatcgaccgc atcgcgctcg
    10981 tgatggaggc gaccgtgccg ggcatcgact acgccaagtt ccaggaaatc gccgaaatct
    11041 cgaagaagaa ctgtcccgtg tccaaggccc tggccgccgt gcccgagatc acgctggagg
    11101 cgaagctgcg cacttgaggc cgggccgttt atcatgggtg cccccgacgg agacacgcat
    11161 gaagaattcc acgccgcacg ccctcgaggc gctcctcatc gaatgagtcg cggcgccaca
    11221 atgacgtcgc tggcaacctc gtcattcgat tccgccgcgg tcgcacggcg cctgcttcgc
    11281 gctcataagg gcgccgcggc cgtgccgcat gccgaggtga tcggcccgga tgcggcggcc
    11341 gcttacgcgg tgcaggacgc gaccgtgcga gggctcgggc cggtgggcgg ctggaaggtc
    11401 ggggccaagg gcccgtcggc cgagcccgct tgcgcgccgc tgccggccgg cggcctgttc
    11461 ccgagcggca ccaccctgct gggcccgccg tggcgtatgc gcggcatcga gctcgaggtg
    11521 gcggtgcgcc tgcgccgtga cctggtaccc gggccgggcg agcccgaagc ggccatgctg
    11581 cgagacgcca tcgaggccgt gctgcccgtc atcgaagtgg tcgagacccg gctggccgat
    11641 tggcgcgaca gccccttgct ggcccagttg gccgatctgc aaagccatgg cgggcttgtc
    11701 ctcggcgcgg cccgcgcgct gagccccgcc gagctggacc tgcgcacggt ggaggcttac
    11761 ctcgcattcg acgggcagcc ggtcgccagc acacgcggcg ccaacccggc cggggatatc
    11821 tggcgcctgc tcggctggct ggcccggcat tgcgcgcagc gcggccagcc gctgcgggcc
    11881 gggcaggtcg tcaccaccgg ctcgtgcacc ggcatgttgt tcgcgcccga gggcgcgcat
    11941 gttcaggccc aattggcggg catcgggctg gtcgagttgc gcttctagaa atcgtcccag
    12001 cggcccgtga aaggatgcaa cttctcaaac gagtagctga ccgccgaacg tacttggctc
    12061 gctaccgttg cgcggcgatc ttcgccagca aggcctcccg ccgcgccagg ctttcggccg
    12121 gccagtactg gctgcggtgg tagtactcgg gcaccgcagg ggtgcccggc ggcagtacca
    12181 cccagggctg cttcgaggca gtgaagatat ggatatcggg cggcagctgg tcggggtcgt
    12241 ccagggtacc cacacgcacg aagcgcaccg aaggcccggc gcccgaatag ttgctccaca
    12301 ccgcgatgcg gcacttgggg caacgggcaa tccgctggcc cttgccgctg gccgagggcg
    12361 tgtcgacgat ctcgggctcg gcgcccagca gcaccacgcg gtcggcttcg atcatcgcgt
    12421 tcagcgcaaa ggctgagccg gtctcgcgct ggcaccagcg gcagtggcaa caatgaacga
    12481 agagcgggcg cgtttccatg cggtagcgga tggcgccgca gtcgcagccg cctgtgaggg
    12541 aggacatgga cggtcctttc tgcattcgga tattccggcg aacgcaggtc ccggcctatg
    12601 ggccggagtt ctcgcgctgc gcgtgctccg cgcaataacg gatgaattcc gaagcctcgc
    12661 tcttgtcgag cacggcgtcg gcacccaggt ggaggcagtg tttgcgcacg cccggtgttg
    12721 cgtagctgct gaggaccacg acgcgacccg ctgtggaaag agccttggcc cggggtatca
    12781 cacccatgcc ggtgccctgc tccagcacca ggtcgatcac ggccagatcc cagccattcg
    12841 ggttctcttc cagccacagg ttggcctcgg cttcggtcct gacagtggcg acgatgcgaa
    12901 agccgcccag ggccgcgaac aggtccgcgt acaagccttg cgtagacttg aggtcttcaa
    12961 ccagaaaaac tcgaacctcc gccttgcctc cggcgttttt gcgagcaacc tcaggcattt
    13021 gtgccgcatc gcctcgatcg ccgcccgatg ccggctgcac agctacgata gcggtcagcg
    13081 cgggtgttcg gagacttgac atgccggaag tgtaagcatg gcccataggc agaaccatct
    13141 catcaacatc ccgttacctt ggtcgcgcat gccatgcaac agccgggctc gtgccctgcc
    13201 acatactccg gatcggcgct ttgtgggcat tcccgatgaa ccttcgacaa gtcttcgcac
    13261 ccctcatttt cctcgtcacc gcctggtgcg ttcccgccgc ggctttcgac ctccaggggc
    13321 ataggggcgc acgcggcctc gcgccggaga atacgctggc ggcgttcgag cgcgcgctcg
    13381 aagtcggcgt gaccacgctc gagctggaca tcgggctcac cgccgacggc gtcgtggtga
    13441 tcgcgcacga cccccacctc aacccggcgc tgacgcgcga cggcagcggc cgctggctgg
    13501 ccggcgccaa gggtcccctg ctgcgctcgc tgacgatggc acaactgcag gcctacgacg
    13561 tcggccgcat cgatcccggc acgtcttatg ccgggacttt cgcctcgcag cagccgcgcg
    13621 acggcatgca tattccgtcg ctggcggcgc tgttcgatcg cgtcaaggcg ctgggcgcga
    13681 acgacgtgcg tttcaacatc gagaccaaga tcaacgccaa ccagccgcag gacacggtcg
    13741 gccccgaggc gatggtgggc gcgctgatgc aagtggtgcg tgaggcgggc atgacggccc
    13801 gggtttccat ccagagtttc gactggcgca cgttgcgcct ggtgcggcag ctcgagccct
    13861 ccattcccac ggtgtgcctg acgatccaga ccgcgaagaa cgacaacctg cgcgacggcg
    13921 actggaccgg cggcctgaag ttcgccgatc acggcagtgc gccgaagatg gccaaggcgg
    13981 ccggctgcgc cgtctggtca cccaacggcg gcgccgtgac cgagccgctg gtcaggcaag
    14041 gccgggcgct ggggctcaag gtggttccct ggaccatcaa cgacccggcg gacatggaac
    14101 ggctgatcgg ctggggcgtg gacggcctca tcaccgacta cccggaccgc ttgcgcggcg
    14161 tgatgcaggc gcgcaatctg gcgctcccga aagcgctggc gaactgaggc cgggtatgct
    14221 ctggcctcgg ccgggcgccg gcgcgcggcc gttccaagga gagccatcat gagcaaccat
    14281 atctacaaga gcctcgagct caccggatcc tccacggtcg gcatcgagga tgccgtcagc
    14341 agggcgatcg ccaaagccca cgagaccgtg cgcaacatcc actggttcaa tgtcatcgag
    14401 acgcgcggcc atgtggagga tggaaaggtg gcccattggc aggtgacgct caaggtcggc
    14461 ttcacgctcg aatagcgcat cggcgcgagg agtggcgttc cgccgatgcc cttcatcctg
    14521 tatctcttcg cgctgaccaa cctggtcatc ggcaccggcg ccttcgtcct ctcgggcatc
    14581 ctccagccgc tcgccgactc gctggaagtc agcgtcgcgg cggccggcca ggtgatgacg
    14641 gcgtatgcgc ttgccgcggc cttgctggct ccgctgttcc tgctggcgac cgggcactgg
    14701 ccgcgcaagc gggcgatcct gctggcgctg gccttgttcg ccgcgggctg cgtgctgtgc
    14761 gcgtcggccg gttcgctggc cgtgctgctg ttgggccgcg ccctgatggg cgtcggctcg
    14821 gtttttacag cgctcgcggc aggcgtcgcg gtggcggtgg tggcgcccga gcggcggggc
    14881 cgcgctctgt ccctgacttt tctcggcatg agcctgagtt atgcggtggg cgttccgctg
    14941 ggcgcctggc tcggattcac ctatggctgg cgagcgcccg tgtggctggt cgcggcggcc
    15001 tgcgtggccg cgaccgccgt gctgatggcg ctgctgccgc ggcggatcga ggcacccggc
    15061 gcgcgtttcc cgggccttgg cgcggcggcg cggcaatggc cgatcctgcg ggtatggctt
    15121 cgcaccttgc tgttcttcat cgcgatcttc agcgtcttcg cctacgtcgg gccggtgctg
    15181 ctggcgctga accccctgac accggcacaa ctgtccttca cgctggtgat gttcgggctc
    15241 tcgggcgtgg ccggcaccat catcggcggg ctggcggtgg accggttcgg ccccgttctt
    15301 accataggtg tgcagatcgc gctgctggcc tcgatgatgc tgctggtgcc gctcacgcgc
    15361 ggccaccacg cgctcacggt gctggtgttc gtcgtctggg gcatggccgg cttcggcctg
    15421 acggcgccgc agcaggtgct gctggtggcg cgctcgcccc agcaggcgcc gctgctgata
    15481 agcctgaatg gctcgatgct ctacgtgggc acggcgctcg gcgccgccgt gagcggcgcg
    15541 ttcgccgcgt cgctgggttt cgacaagctg tcctggatcg ggctgccttt cgcactactg
    15601 gcgctggcga cgttatggtt cgatacacaa gggcgcatgc atccggcaca ctgaggcctc
    15661 aacgttcgtt cggcgacagc ggcctgctcg accagctgca gcagtggatc gacgaagcgc
    15721 gggcgcaact ggattcgacc ggcgcgccgc cctgatttcc cgagcgcggc gtgccgcatc
    15781 aatgcccgtg ccgatggtgc gcatccggga agtgcggatg gctgtgcacc atgggctggt
    15841 gccggtgcgg gtggctgtgc cgggtgccgg gttcgaccgg cggctgatgc gtatggtcgt
    15901 gatggacgtc gcccgcgccg tgcacgtgct cgtgcgtatg ctcgatcgcc tcgtgcgcat
    15961 ggcggtgttc atggcgctcg gacagatgca gcgcaacgcc gaccgccatc agcaaaccgg
    16021 ccgcgagcag cggcgcggtg accgcttcgc ccagcagcac gatggccagc acggccccga
    16081 aaaaaggcgc caccgaaaag tacgcgccgg tgcgcgcggt gcccagttcg cgcaggccga
    16141 cgacgaacag cgccaggctg gcgccataac tggcgaagcc caccagcgcc gccgcggcca
    16201 ccaccgccgg cgcgggccag gtggcgccca acgccaaggc cagcaccaga ttggtcgcgc
    16261 ccgccgcaag acccttgacc attgcgatcc aggacgcatc ggccagcgac accttgcgcg
    16321 tgaggttgtt gtcgatcgcc caggcaagac acgcgccggt gaccagcagc gcgctctgcc
    16381 attgcggcaa ggtcatttgg ccgggccacg ccagcaccag gccgccggcc acgatggcga
    16441 gcatgccgag cgccacgcgc cgatcgaagt tctccttgaa gacgaaccag gccagcaagg
    16501 ccgtgaatac cgcttcggca ttgagcagca gcgaagcgcc ggaagccggc atgcccgcca
    16561 gtcccagcat cagcagcacc ggccccgcga caccgcccgc gacgatcgcg ccggccagcc
    16621 acttggcctc gccggccggc agccgcacgc gcccagctcg ccggatgcgg cgcagcagcg
    16681 ccaggcccag gcccgagccc aggtagagca aagcggccag cagccacggg ctcgcctgcg
    16741 ccagcagcag cttggccagc ggggtgccgg ccccgaacag cagggccgcc gacaagctgg
    16801 ccagcacccc gcgcggcaag ccgccccctg aagtcattgc ccggtgccgt ccgtgtggca
    16861 ggcacaggcc tcgccatgtg ccgccgccac cagttcgtgc aggatgccgc actcgtgcgt
    16921 cgcatgatcg gcgtcgcagc tgtcacgcag cgccgccagt tgccgctcga gcgcgcgcag
    16981 gctggccagg cgcgcgcgca cgcgcagcaa ctgcgcatcg atcagcgcgt cgatgtcgcc
    17041 gcaatcgtcg cgcgcgccac cgacgaagtc caacagccgc ttcacgtcgg ccagcggcat
    17101 gtccagggcg cggcagtgac gcacgaaggc cagccgctcc aggtgtggtg cgccgtagct
    17161 gcgatagccg ttggcttcgc gcgccggcgc cggcagcagc ccggccttct cgtaatagcg
    17221 gatggtttcc acctcgacgc cggtggcgcg gctcaaatcg ccgatcttca tggcatcaat
    17281 gcagcaggca gtggcaatag acgaactgct gcgccgtgcc ggacggcgtg cggtgctgct
    17341 ccgccacgtg atcgagcagc tcgaactggc cgccgaagcc aatgcgattt cggttccatg
    17401 cgaactccat gtcatggaca gggctttcat gctaacgcgc ttgaccttga agcggcttca
    17461 gggtttccaa tgaccttgac gcataacaag gaatgacgtg tcagcgcatt gccagcactg
    17521 ccagccccag cacgacgacc cccaccggcg ccccgcgcca ggctaccggc gcgtgctgtg
    17581 gatcgcattg ctcatcaatg cctcgatggt cggcgtggaa atcctggccg ggctgagctc
    17641 gggttcggtc tcgctgctgg ccgacgcgat cgacttcttc ggcgacgcgg ccaactacgg
    17701 cgtcacgctt gccgtgctga acctgggcct ggcgtggcgc gcccgtgccg cgctgctcaa
    17761 gtcggcgagc atgttggcgt ttggagggat cgtgctggcc aaggccttgt gggccatgag
    17821 ccagggagtg ccgccggagg ctttgacgat gggcctggtg ggtgcagcgg cgctggcggc
    17881 caacctgggt gtggcggcgc tgctttatgc ttttcgcagc ggcgacgcca atatgcgcgg
    17941 cgtgtggctg tgtacgcgta atgacgcact cggcaacctt gccgtgatgc tggcggctct
    18001 gggtgtgttc cgtaccggca cggcctggcc cgacctcgtc gtggcaatcg tgatggcggg
    18061 ccttgcgatc agttcgggat tgtcagtgat gcgcca
//
LOCUS       DASWIY010000269         3785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_910337, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000269 DASWIY010000000
VERSION     DASWIY010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910337"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VGA59_09965"
     CDS             <1..146
                     /locus_tag="VGA59_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX9720044.1"
                     /translation="AVFENFDEFKKLHPALANLDPKKMVKDGVSAPMHPGALKYYKEK
                     GWL"
     gene            254..2869
                     /locus_tag="VGA59_09970"
     CDS             254..2869
                     /locus_tag="VGA59_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEX9720045.1"
                     /translation="MFKRKSGEAAAVDVKKLVAATDLGGRSPGPLTARMLLGVALAWS
                     LFQLWIASPLPFVFGMFVLNDTESRSIHLAFAVFLAYMAYPASKYSPSDHIPVTDWVF
                     ALAGAFCAAYLFLFYRELATRPGQPTTMDIVVAAAGVLLLLEATRRVLGLPMVVVAVV
                     FLIYTFGGRYMPDMIAHKGHSLARVASHQWLTTEGVFGVALGVSSGFIFLFVLFGSLL
                     DKAGAGNYFIKSAFALLGHMRGGPAKAAVVSSAATGIISGSSIANVVTTGTFTIPLMK
                     RVGYRPDQAGAVEVSSSVNGQLMPPVMGAAAFLMVEYVGIPYTQVIKHAFLPAIISYI
                     ALFYIVHLEALKSDLKGLPRRGNTTALQRLRSLAMTVAGFVVLSGIVYWGIGWLKAVL
                     GDAAIAVVGALVLGAYVGLLWISARQPELVLDDPNAPVFELPETAPTLKSGLHYLLSV
                     VVLIWCLMVEQMSPGLSAFWATMFMVFIMLTQRPILVLLRGEGVMGAALRRGFAELID
                     GLATGARNMIGIGVATAAAGIIVGTVSLTGIGLVMTEVVELVSGGNLMLMLVMVALIS
                     LVLGMGLPTTANYIVVSTLMAPVVVELGAQGGLLVPLIAVHMFVFYFGLMADVTPPVG
                     LASFAAAAIARTDPIKTGVTAFYYSMRTAILPFLFIFNTQLLLIGLDSWWHLGFTVIT
                     AVVAMLVFAAATQGYFLVRTRWYETVALLLVTFTLFRPGYWWDMFYPPYVEAPAAELM
                     KLVQEAPPNMGKRVWVEGEKRDGGEVRKGVLLPLGAPGDARERLGRIGLRVTSFGGAV
                     QVSQVEFGSKAAKLGLAPGQKIVSIELPAERPPKEWMALPAAALLLLVMGLQRARGRR
                     ESDAHAATLGATPGS"
     gene            2866..3510
                     /locus_tag="VGA59_09975"
     CDS             2866..3510
                     /locus_tag="VGA59_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX9720046.1"
                     /translation="MTRIALIHALAHSIAPINEAMVRDWPPATRMNLLDDSLSADLAA
                     GSGLDAAMHERFERLAQYAVDCGSHAILFTCSAFGPCIEAVARNHAPLPVLKPNEAMV
                     ADAAAIELRVGLVASFGPTLQSMPPEFPAGVELECALAGGALAALNAGDPQLHDDLIV
                     RTALELARRGCKAIALAQFSMARARSRVAAETGLPVLTTVDSAVAALKRRLGVG"
     gene            complement(3671..>3785)
                     /locus_tag="VGA59_09980"
     CDS             complement(3671..>3785)
                     /locus_tag="VGA59_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX9720047.1"
                     /translation="LSIEQGRPTKRGRRDLDDAQRGWGDRWSASLDDEGKT"
BASE COUNT          547 a         1237 c         1312 g          689 t
ORIGIN      
        1 aggcggtgtt cgagaatttc gacgagttca agaagctgca tccggcactg gccaacctgg
       61 accccaagaa gatggtcaag gacggcgtgt ccgcgcccat gcacccgggc gcgctcaagt
      121 actacaagga aaagggctgg ctatgattca agcatgacgg cgcggcgcgg gcagccctgg
      181 cagcccgcga cgggcgctgc gtcaggccaa agccccgcgg agccggggct ttttcttttc
      241 gaggagacga gcaatgttca agcgcaagtc cggtgaagcc gctgccgtgg atgtgaagaa
      301 gctggtggcg gccaccgacc tgggtggacg cagcccgggc ccgctcacgg cccgcatgct
      361 gctgggagtg gcgctggcat ggtcgctgtt ccagctgtgg attgcctcgc ctctgccttt
      421 cgtcttcggc atgttcgtgc tcaacgacac cgaatcgcgt tccatccatc tggcgttcgc
      481 cgtgttcctg gcgtacatgg cgtatcccgc ctcgaaatac tcgccatccg accacattcc
      541 ggtgaccgac tgggtgttcg ccctggccgg cgctttctgc gccgcctacc tgtttctctt
      601 ctaccgcgag ctggccacgc ggcccggcca gccgacgacg atggacatcg tcgtggccgc
      661 cgcgggggtg ctgctgctgc tggaggccac gcgccgcgtg ctgggcctgc ccatggtggt
      721 ggtggccgtc gtgttcctga tctacacctt cggcgggcgc tacatgcccg acatgatcgc
      781 ccacaaggga cattcgctgg cccgtgtcgc ctcccaccaa tggctgacga ccgagggcgt
      841 gttcggcgtg gcgctggggg tgtccagcgg cttcatcttc ctgttcgtgc tgttcggatc
      901 gctgctggac aaggccggcg ccggcaacta tttcatcaaa tcggccttcg cgttgctggg
      961 tcacatgcgc ggcggaccgg ccaaggcggc cgtggtgtcg tcggcggcca cgggcatcat
     1021 ctcgggatcg tccatcgcca atgtggtgac aaccggcacc ttcaccatcc cgctgatgaa
     1081 gcgcgtggga taccggcccg accaggccgg cgcggtggag gtgtcgagct cggtcaacgg
     1141 ccagctgatg cctccggtga tgggcgcggc cgcgttcctg atggtggagt acgtgggcat
     1201 cccctacacc caggtcatca agcacgcatt ccttccggcc atcatctcct acatcgcact
     1261 gttttatatc gtgcacctgg aagcgctgaa gtcggacctc aagggcctgc cgcggcgcgg
     1321 caacaccacg gcgctgcagc gcctgcgctc gctcgccatg accgtggccg gcttcgtggt
     1381 gctgtccggc atcgtctact ggggcatcgg ctggctcaag gccgtgctgg gcgacgcggc
     1441 catcgcggtg gtgggcgctc tggtgctggg cgcctacgtc ggcctgctgt ggatcagcgc
     1501 gcgccagccc gagctggtgc tggacgaccc caacgcgccg gtgttcgaac tgcctgaaac
     1561 cgcgcccacg ctcaagtcgg gtctgcatta cctgctctcg gtggtcgtgc tgatctggtg
     1621 cctgatggtc gagcagatgt cgccgggcct gtctgccttc tgggccacca tgttcatggt
     1681 gttcatcatg ctgacccagc ggccgatcct ggtgctgctg cgcggcgagg gcgtgatggg
     1741 cgccgcgctg cggcggggtt tcgccgagct gatcgacgga ctggccacgg gcgcgcgcaa
     1801 catgatcggc atcggcgtgg cgacggcggc ggcaggcatc atcgtgggca cggtgtcgct
     1861 caccggcatc ggcctggtga tgaccgaagt ggtggagctg gtctcgggcg gcaacctgat
     1921 gctgatgctg gtgatggtgg cgctgatcag cctggtactg ggcatgggcc tgccgaccac
     1981 ggccaactac atcgtggtct ccacgctgat ggcgcccgtg gtagtcgagc tgggcgcgca
     2041 gggcggcctg ctggtgcccc tgatcgcggt gcatatgttc gtgttctatt tcggcctgat
     2101 ggctgacgtg acgccgcccg tgggattggc ttcgttcgcg gccgcggcca tcgcccgcac
     2161 cgacccgatc aagaccggcg tcacggcgtt ctactacagc atgcgcacgg ccatcctgcc
     2221 gttcctgttc atcttcaaca cccagttgct gctgatcggg ctggacagct ggtggcacct
     2281 ggggttcacc gtgatcaccg cggtcgtcgc catgctggtg ttcgcggcag ccacccaagg
     2341 ctacttcctg gtgcgcacgc gctggtacga aaccgtggcg ctgctgctgg tgaccttcac
     2401 gctgtttcgg cccggctact ggtgggacat gttttacccg ccctatgtgg aggcgccggc
     2461 ggccgagttg atgaagctgg tgcaggaggc gccgcccaac atgggcaagc gcgtgtgggt
     2521 ggagggcgaa aagcgcgacg gcggggaggt cagaaaaggc gtgttgctgc cgctgggcgc
     2581 tccgggcgac gcgcgcgagc ggctgggtcg catcggcctg cgggtcactt cgttcggcgg
     2641 cgcggttcag gtttcgcagg tggagttcgg ctccaaagcc gccaagcttg gcctcgcgcc
     2701 ggggcagaag atcgtcagca tcgaattgcc cgccgagcgc ccgcccaagg agtggatggc
     2761 gctgccggcg gcggcgctgc tgctgctggt gatgggactg caacgcgcgc gtgggcgccg
     2821 cgaatcggat gcgcacgcgg cgaccctggg agcgaccccg ggctcatgac acgaattgcc
     2881 ctgatccacg cgctggccca ttcgatcgcg ccgatcaacg aggccatggt gcgcgactgg
     2941 ccgccggcca cgcgcatgaa cctgctggac gacagcctgt cggccgatct cgctgccggc
     3001 agtggactgg acgccgccat gcacgagcgc ttcgagcggc ttgcccaata cgccgtcgat
     3061 tgcggcagcc acgccatcct gttcacctgt tcggccttcg gtccctgcat cgaagccgtg
     3121 gcccgcaacc acgccccatt gcccgtgctc aagcccaacg aggcgatggt ggccgacgca
     3181 gccgcgatag agctgcgcgt cggcctggtg gctagcttcg ggccgaccct gcagtccatg
     3241 ccgcccgagt tcccggcggg cgtggaactc gaatgcgctt tggccggggg cgcgctggca
     3301 gcgctgaatg cgggggaccc acaactgcac gacgacttga tcgtccgcac ggcgctggag
     3361 ctggcgcggc ggggatgcaa agcgatcgcg ctcgcacagt tcagcatggc gcgggcccga
     3421 tccagggtgg cggcggagac cggcttgccg gtgctgacca ccgtcgacag cgccgttgcc
     3481 gcgctcaagc ggcggctggg cgtgggctag gctgggagtc gggcgcggga agtcggggag
     3541 ttgccccctc ttccagaggg agagggagaa aatcccggct ctgccgccca agactccctg
     3601 ggccaagccc gggatgacaa accattgtca tcgcgggccg caccgaggca tcatgctccg
     3661 gcccggccga tcaggttttc ccctcgtcgt ccaacgaagc gctccagcga tcgccccagc
     3721 cgcgctgcgc atcgtcgaga tcacgccggc cgcgcttggt cgggcggccc tgttcgatcg
     3781 acagt
//
LOCUS       DASWIY010000270        12496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_167037, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000270 DASWIY010000000
VERSION     DASWIY010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 12496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12496
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167037"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VGA59_09985"
     CDS             complement(<1..463)
                     /locus_tag="VGA59_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720048.1"
                     /translation="MQLLLLLLLLLLSFLDGLLLAKGLPALLLHSFLGGLPLVKGLLA
                     LELPLLCPALLFLLALHLQLALGTLLLHLLRLFTPRALHLLRLLPALLVLRPLLAALA
                     LVAPDLRRPFLSARIYHRRALFFEAAAWLLRADVLTRPEGGAVASAALDRFR"
     gene            complement(642..908)
                     /locus_tag="VGA59_09990"
     CDS             complement(642..908)
                     /locus_tag="VGA59_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720049.1"
                     /translation="MRTATATAADNPFALMTDPGRVRQMIDRSESLSRLSQRQCHPLD
                     KAVIRSASAELAAYDAKIDRTTVYLPPDEEQSSNLVRSFPEPVN"
     gene            1076..2569
                     /locus_tag="VGA59_09995"
     CDS             1076..2569
                     /locus_tag="VGA59_09995"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX9720050.1"
                     /translation="MPYMPPFIAPTRHDLPESALAQVRAIYSQQIEHLRNAMQRFVAG
                     ESLPGHVRACYPFVRIHTDTVVRQAALENLGLSYGFVAGAGRFETTLTRPDLYGNYYL
                     EQFRLLRQNHGVELEVGTSTQPIPVHFSFAENDHIEGTLSLERRLLMRDVFDLPDLGA
                     MDDGIANGTWEARPGESQPLALFTAARVDYSLHRLRHYTGTAPDWFQNFVLFTNYQFY
                     IDEFVRLGHEEMADPRSDYTAFVEPGNVVSRRAGLGAEAGDELGAVPPRLPQMPGYHL
                     IRPDHGGITMINIGVGPANAKTITDHVAVLRPHAWMMLGHCAGLRNSQQLGDYVLAHA
                     YVREDHVLDEDLPLWVPIPALAEIQVALERAVADVTRCKGADLKRIMRTGTVASTDNR
                     NWELLPGNMPQRRFSQSRAVALDMESAAIAANGFRFRVPYGTLLCVSDKPLHGEIKLP
                     GMANQFYRERVDQHLRIGMRAIEILRAEGSQRLHSRKLRSFDEVAFQ"
     gene            complement(2771..3619)
                     /gene="asd"
                     /locus_tag="VGA59_10000"
     CDS             complement(2771..3619)
                     /gene="asd"
                     /locus_tag="VGA59_10000"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349243.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEX9720051.1"
                     /translation="MSNRLAVTPQYLLPKRVLTVLAGKIANLRGGALTHALIRRFVAK
                     YGVDMSEAADPRIESYATFNDFFTRALRDGVRPIAAAPFVCPVDAAVSQFGPIEHDQI
                     FQAKGHSYSTRALVGGDQALAHLFDHGHFATLYLAPRDYHRIHMPCEGRLKRMIYIPG
                     DLFSVNPLTARHVPSLFARNERVVCFFETAYGPFANVLVGATIVGSVATVWHGVVNPP
                     RTRDIREWRYDDQNIVLPQGAEMGRFLLGSTVVMLFPKNVLTFVGDWAPSRSVRLGEA
                     MATALR"
     gene            3698..4621
                     /locus_tag="VGA59_10005"
     CDS             3698..4621
                     /locus_tag="VGA59_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9720052.1"
                     /translation="MSEFLFEAAGLTKRYGQSTVVDDVSFEIARGECLGVIGPNGAGK
                     TTTIRMCLGLTVPDHGSIKALGLSMPRDELAIKAQLGVVSQFDTLDPDFTCAENLLVY
                     GRYFGMKDAQIRERIPRLLEFAALSHKANARPGELSGGMRRRLSLARALVNDPRLLLL
                     DEPTTGLDPQARHLMWERLQLLLQQGKSILLTTHFMDEAERLCSRLLVLDHGRKIAQG
                     RPRDLIAEHLEPDVVEVYGNGALELVESDLKKLAARVEVSGETVFFYTQHAKPLLDAM
                     ADHPKLRTLHRPANLEDLFLKLTGRQIREEA"
     gene            4817..5656
                     /locus_tag="VGA59_10010"
     CDS             4817..5656
                     /locus_tag="VGA59_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9720053.1"
                     /translation="MSALAPSVTAQGVSIWRAPDLSFRWWPVFLRNLLVWRKLAIPSL
                     VGNIAEPLMWLVAFGYGMGALVGQVSITTTSGATTVPYILFLASGSICMSAMNAASFE
                     ALYSAFSRMHVQKTWDGIMNAPVNLDNVVMAEMLWAGFKALFSVTAILCVMLALNISH
                     SPKLLAAWPVLLGVGITFSCIALIFNALAQGYDFFTYYFTLFLTPMMFLSGVFFPREQ
                     LPDALRAFSDWLPLTSAVELVRPMFMDQWPAHPARNALVLVAYTVVAFWVALALTRKR
                     FRA"
     gene            complement(5631..7274)
                     /locus_tag="VGA59_10015"
     CDS             complement(5631..7274)
                     /locus_tag="VGA59_10015"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEX9720054.1"
                     /translation="MDTDFDYIIIGAGSAGCVLAGRLSEDPGVKVALLEAGPPDRSVL
                     IHCPAGLALIAKTGQANWAFSTVPQPGLNGRRGYQPCGKVLGGSSSVNAMIYIRGHHS
                     DYDRWAAEGNEGWGWDDVLPYFKRAEHNERGADEFHGVGGPLNVMDLRSPNRFGPVFV
                     QAAVQAGYPQNPDFNGVQQEGFGLYQVTHRNGERFSAAKAYVAPNLGRPNLKVITDAH
                     TTRILTEGRRVIGVEYHQEGQLKRLKAAREVLLSAGALQSPKLLMLSGIGPGAQLHAH
                     GVPVVQDMPGVGRNLHDHPDVIQVVDAPQLAELFGLWPSTVGPLVRSIFEWRRSRSGR
                     LTTNFAEAGGFIKSGPGVPLPDLQLHFVVAKLVDHGRKTVFGLGYSCHVCLLRPDSRG
                     SLRLASNDPMVAPQIDPNFLGERSDIERLVRGFKITRNILAQSALADYGGRELAASAA
                     AQSDEEIEQFIRDRADTIYHPVGTCRMGSGALDVVDSQLRVRGVDGLRVVDASIMPHI
                     VAGNTNAPIIMIAEKAADMIKAAARRGAEAVLRPGSVCA"
     gene            7574..8020
                     /locus_tag="VGA59_10020"
     CDS             7574..8020
                     /locus_tag="VGA59_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9720055.1"
                     /translation="MAHKIDPEQALLLDNQLCFALYSTSLAMTKLYKPLLDELGLTYP
                     QYLAMLVLWERDGLMVSELGERLFLDSGTLTPLLKRLESAGLVARIRDVQDERRVHVT
                     LTAAGRKLKAKAQKVPGCVLSASQCSIPELVQLTRQLRGLRQRLAG"
     gene            8089..8505
                     /locus_tag="VGA59_10025"
     CDS             8089..8505
                     /locus_tag="VGA59_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX9720056.1"
                     /translation="MSQLEKVLYTAKAHTTGGRDGASRTDDGRLDIKLSPPGMGGSGT
                     NPEQLFAAGYSACFIGALKAVAGKMKMTLPADLAVDAEVDLGPVAGGYGIAARLNVSL
                     PGFERAAAQALVDAAHKVCPYSNATRGNIDVKIKLV"
     gene            8645..9394
                     /locus_tag="VGA59_10030"
     CDS             8645..9394
                     /locus_tag="VGA59_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX9720057.1"
                     /translation="MKLALLSDLHANRQALEACLADARARGATQYAFLGDVVGYGGEP
                     AAVLDRVMSMAGDGAWVVRGNHDEAAIKPLPGSRRADHTGAAWTRAQLNPAQQEFLAA
                     LPLMLRQDFILLVHASAREPQRWAYVEDATGATASLDAAVVEGATHVFGGHVHEQRLF
                     FQGTGRGAMGFEPTAGVPIPLPRHRRWLATVGSVGQPRDGRRDAMYALFDSTSTRLVF
                     LRVPYDHEAAARAIRQAGLPEFYAQRLALGR"
     gene            9418..10911
                     /locus_tag="VGA59_10035"
     CDS             9418..10911
                     /locus_tag="VGA59_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/universal stress protein"
                     /protein_id="HEX9720058.1"
                     /translation="MKQLNPGTEIDGFLIGECVHAGGMAHVYRVRYSRAGHDPGFPMA
                     MKVPRMTSGDGAENIVSFEVEHQMLQALSGPHVPRLVATGDMARLPYLVMEYIEGRTL
                     QHWLDRASEDRTRPSAQDIARLGAATAVAAHSLHQQEAVHLDLKPGNVMIRPDGGAVL
                     LDFGLSWHGHYPDLLAEEMRSAIGSPPWIAPEQVVGVRGDPRSDVFAIGVMLYELATG
                     KLPFGNPQTRGGLRQRLWMDPRPPRLLAPETPSWLQEIILRCLEPEADDRYASAAHLA
                     FDLAHPDQVQVGARGEKTRRTSWRAHAKRWLKAAGLHYHPSPLPSSQVEQVPIVMVAV
                     PWKDASDATLYSLRRAASRSLGIRPGARLACVTVIAPGDTDPTDDRCSEVQVRRWQLD
                     RLRQWAQGLDLQGHRLSFHVLEAGDTAQALLEYARANRVTLMILGAATHGLKMQRLIA
                     TVPVRVAMDAPCSVMLVKQSLPFERLGENSATADAPAATMGHVVSSL"
     gene            10892..11893
                     /locus_tag="VGA59_10040"
     CDS             10892..11893
                     /locus_tag="VGA59_10040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX9720059.1"
                     /translation="MSSHPYEVLTPDVVMDALAFVGLRGDGRLMALSSYENRVYQAHL
                     EEPVEGHGAVVAKFYRPGRWSEAQILEEHAFSAELAAAEVPAVAPLLLNGSTLHEHGG
                     FAFSVSPRRGGRAPELDDAEVLEWIGRFLARIHMVGASRPFATRPALDLRSFGQEPRD
                     WLLEHHMIPLDVQAVWERRCDEALRMIKDTALGDGAAGGMAMIRLHGDCHPGNILWTP
                     DQGPHFVDLDDARTGPAVQDMWMLLSGERAQRQRQLGPLVDGYELFREFDRNELALIE
                     PLRTLRLIHYSAWLARRWDDPIFPINFPWFGSSDYWQGQVQMLEEQIEAMAEPPLVV"
     gene            12050..12385
                     /locus_tag="VGA59_10045"
     CDS             12050..12385
                     /locus_tag="VGA59_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9720060.1"
                     /translation="MPSIKTIPFEVSQSLVVLGQRVKKARMRRQLSQEELAQACKIAR
                     RTLFSIEAGAPGIAVGHIYTVLWKLGLLATTRGVADPDADEHGKILEAAKLGKRVRRP
                     QDKAEDNNF"
BASE COUNT         2113 a         4184 c         4132 g         2067 t
ORIGIN      
        1 gcctgaatcg atcgagcgcg gcgctcgcaa ccgccccgcc ttcaggccgc gtgaggacat
       61 ccgcgcgcaa gagccacgcc gccgcctcga agaacagcgc ccgccggtgg tagatccgcg
      121 ccgacaggaa cggccgccgc aggtccgggg cgacgagcgc caacgcggcg agcagcgggc
      181 gcaggacgag cagcgccggc agcaggcgca gcagatgcaa cgcgcgcggc gtgaacaggc
      241 gcagcaggtg cagcaggagc gtgcccaacg cgagctgcag gtgcagcgcc agcaggaaga
      301 gcagcgccgg gcacagcagg ggcagctcca gcgcgagcag gcccttcacc agcggcaagc
      361 cgcccaggaa cgagtgcagc agcagcgcgg gcaggccctt cgccagcagc aggccgtcca
      421 ggaacgacag cagcagcagc aacagcagca acaacagttg caagggcggc cgcaggttgt
      481 cgctccggcg gcggtccggc cgggtccgcc gcaacgcggc gaagcgcgcg aacgcgccgc
      541 ggaacgcggc cgcgacgtcc aggagcggcg cagcgagcgc cgctcacagg gcgatgacga
      601 tcgccgcggg cgcggccagg gtcaggggcg cggaggctga atcagttgac cggttcggga
      661 aacgagcgga ccaggttcga ggattgttct tcgtccggcg gcaggtagac cgtcgtacgg
      721 tcgatcttcg catcgtaggc cgccagttca gcgctggcgc tgcggatcac ggctttgtcc
      781 aatggatggc actggcgctg gctcaatctg ctcagggact cggatcggtc gatcatctgg
      841 cgcacgcgcc cggggtccgt catcaacgca aagggattgt cggccgccgt tgctgttgct
      901 gttctcattc gctgaactcc tgtagatgca gtgaaaccgc gtttcgatgt gggcttactt
      961 tcgcaggaga gcggcgtttg taacgtcagg tggtttcgtt acaccttgta ggacggcttc
     1021 tcacgctggg gcaggcgccg gcaagggctg cgttgcgcgt tgcacaatag gattcatgcc
     1081 ttatatgccg cctttcatcg cgcccacccg gcacgacctt ccggagagcg cgctggcgca
     1141 ggtgcgtgcc atctattcgc agcagatcga gcacttgcga aacgcgatgc agcgtttcgt
     1201 ggccggggaa tccctcccgg gacatgtccg ggcctgttac cccttcgtcc gtatccatac
     1261 cgacacggtg gtgcgtcagg cggcgctgga aaacctggga ctcagttatg gcttcgtggc
     1321 cggcgccggc cggttcgaaa ccacgctcac ccggcccgac ctgtacggca actattacct
     1381 ggagcagttc aggctgctgc ggcaaaacca cggcgttgaa ttggaagtgg gcaccagcac
     1441 acagcccatc cctgtccatt tttcgttcgc ggagaacgac catatcgagg gaactctcag
     1501 cctcgagcga cgcttgctga tgcgcgacgt cttcgacctg ccggacctgg gtgcgatgga
     1561 cgacggcatc gccaacggca cttgggaggc gcggcccggc gagtcgcagc cgctggcgct
     1621 tttcaccgcg gcacgggtcg attactcgct gcaccggctg cgccactaca ccgggacggc
     1681 gccggactgg ttccagaatt tcgtgctgtt caccaactac cagttctaca tcgacgaatt
     1741 cgtgcgcctg ggacacgagg agatggccga tccccgcagc gactacaccg ccttcgtcga
     1801 accgggcaac gtggtgtcgc ggcgcgccgg attgggcgcc gaggccgggg acgagctggg
     1861 agcggtgccc cccaggctgc cgcagatgcc gggctaccac ttgatccggc ccgaccacgg
     1921 cggcatcacg atgatcaaca tcggcgtggg accggccaac gccaagacca tcaccgacca
     1981 cgtggccgtg ctgcgcccgc acgcctggat gatgctggga cactgcgcgg gcctgcgcaa
     2041 cagccagcag ctgggcgact atgtgctggc gcacgcgtat gtgcgcgagg accatgtgct
     2101 ggacgaggac ttgccgttgt gggtgcccat tcccgctttg gccgaaatcc aggttgccct
     2161 ggagcgggcg gtagccgacg tcacgcggtg caaaggggcc gatttgaagc ggatcatgcg
     2221 cacgggcacc gtggccagca ccgacaaccg caactgggaa ctgctgcccg gcaacatgcc
     2281 gcagcgccgg ttcagccaga gccgcgccgt ggcgctggac atggaaagcg ccgccatcgc
     2341 ggccaacggc tttcgcttcc gcgtgcccta cggcaccctg ctgtgcgtga gcgacaagcc
     2401 cctgcacggc gagatcaagc tgcccggcat ggccaaccag ttctaccgcg agcgcgtcga
     2461 ccagcacctg cgcatcggca tgcgcgccat cgagatcctg cgtgccgaag gcagccagcg
     2521 gctgcacagc cgcaaactgc gcagcttcga cgaggtggcg ttccagtagt gaaatgtcat
     2581 tgcgggccgg gctcgcggaa cgcccggggc gcgcagctgt ccagtgcagc gagcccattg
     2641 ccgggcctac gctatgcacg taccgaggca tcacgctccg gcccggcccc gcaatccatg
     2701 gaccccggat caggcctgcc ccggacttga tccggggtcc gggatgacag cctcctggat
     2761 gacaactccg tcaacgcaaa gccgtcgcca tcgcctcacc cagccgcacc gatcgcgacg
     2821 gcgcccagtc gcccacgaaa gtcaggacgt tcttcgggaa cagcatgacg acggtggagc
     2881 caagcaggaa gcggcccatc tcggccccct gcggcagcac gatgttctgg tcgtcatagc
     2941 gccattcgcg gatatcgcga gtgcgcggcg gattgacgac gccatgccat acggtggcga
     3001 cgctgcccac gatggtggcg cccaccagca cgttggcgaa ggggccgtag gcggtctcga
     3061 agaagcagac cacacgctcg ttgcgcgcga acaggctggg cacatggcgg gccgtgagcg
     3121 gattgaccga aaacaggtcg cccgggatgt agatcatccg cttcaggcgg ccctcgcagg
     3181 gcatgtggat gcggtgatag tccctgggcg cgaggtagag cgtggcgaag tgtccgtggt
     3241 cgaacaggtg cgccagcgcc tggtcgccgc ccaccagcgc gcgcgtcgag tagctgtggc
     3301 ccttggcctg gaagatctgg tcgtgctcga tcgggccgaa ctggctgacg gcggcgtcca
     3361 ccggacagac gaatggcgcc gcggcgatcg gtcgcacgcc gtcgcgaagc gcccgggtga
     3421 agaagtcgtt gaaggtcgcg tagctctcga tgcgcggatc ggcggcctcg ctcatgtcca
     3481 ccccgtactt cgcgacgaac ctgcgaatga gcgcatgggt cagcgcgccg ccgcgcaaat
     3541 tggcgatctt gccggccagc acggtgagca cccgcttggg cagcaggtat tgcggcgtca
     3601 ccgccaggcg attggacacg tagcgcaccg ggagttcatg aaccgaaaat tctaggccac
     3661 cacggcgtcc tttcgggccg tgcgacacaa taaagccatg tccgagttcc tgttcgaagc
     3721 cgcgggcctc accaaacgct atggccagag caccgtggtc gacgacgtgt cgttcgaaat
     3781 cgcgcgcggc gaatgcctgg gcgtgatcgg cccgaacggg gccggcaaga cgaccacgat
     3841 ccgcatgtgt ctggggctca cggtcccgga ccatggttcg atcaaggcgc tgggcctgtc
     3901 catgccgcgc gatgagctgg ccatcaaggc acagctgggc gtggtcagcc agttcgacac
     3961 gctggacccg gatttcacct gcgcggagaa cctcctcgtc tacggccgct acttcggcat
     4021 gaaggatgcg cagatccgcg aacgaatccc gaggctgctg gagttcgccg ccttgtcgca
     4081 caaggccaac gcgcggccgg gcgaactttc aggcggcatg cgcaggcggc tcagcctggc
     4141 gcgggccctg gtcaatgatc cgcgcctgct gttgctggac gaaccgacga ccgggctgga
     4201 cccgcaggcg cggcacctga tgtgggaacg cctgcaactg ctgctgcagc aaggcaagtc
     4261 gattctgctg acgacgcact tcatggacga ggcggagcgg ctgtgctcac gcttgctggt
     4321 gctggaccat ggccgcaaga tcgcgcaggg ccggccgcgt gacctcattg ccgagcatct
     4381 cgagcccgac gtggtggagg tgtacggcaa tggcgcgctg gagctggtcg agtcggatct
     4441 gaagaagctc gccgcccggg tggaggtcag tggtgaaacc gtgttcttct acacgcagca
     4501 cgcgaaaccg ttgctggatg cgatggcgga tcaccctaaa ctgcgcacct tgcaccgccc
     4561 ggccaatctg gaggacttgt tcctcaaact caccggccgg cagatcagag aggaagcttg
     4621 agatgggaaa agatgtcatg ccgggctcga cagagcctgc cccggacctg atccgggggc
     4681 atccaaggcg gcgggccggg caggcatgga ttgcgggtca ggcccgcaat gacaaggggt
     4741 tgctgtcatc ccggactcga tccgggatcc atgaattcga gccatggatt gcgggtcaag
     4801 cccgcaatga caccccgtga gcgccctcgc cccttccgtg actgcacagg gcgtttccat
     4861 ctggagggcg ccggacctgt cgtttcgctg gtggcccgtg ttcctgcgca acctgctggt
     4921 atggcgcaag ctggccatcc ccagcctggt gggcaacatc gcggagccgc tgatgtggct
     4981 ggtggctttc ggctatggca tgggcgcgct ggtggggcag gtcagcatca ccaccacgag
     5041 cggggcgacc acggtgccct acatcctgtt cctggccagc ggctcgatct gcatgagcgc
     5101 gatgaacgcg gcatcgttcg aggcgctgta ttcggccttc tcgcgcatgc atgtgcaaaa
     5161 gacctgggac ggcatcatga atgcgccggt gaacctggac aacgtggtga tggccgagat
     5221 gctgtgggcc ggcttcaagg cgcttttcag cgtgacggcg atcctgtgcg tgatgctggc
     5281 cctgaacatc agccacagcc ccaagctcct ggcggcatgg ccggtgttgc tgggcgtggg
     5341 catcaccttc tcctgcatcg cgctgatctt caacgcgctg gcccagggct acgatttctt
     5401 cacttactat ttcacgctgt tcctcacgcc gatgatgttc ctttcgggcg tgttctttcc
     5461 ccgtgagcag cttcccgatg ccctgcgcgc attttccgac tggctgccgc tcacaagcgc
     5521 ggtggaactc gtgcggccga tgttcatgga ccaatggccg gcccatccgg cgcgcaacgc
     5581 cctggtgctc gtagcctaca cggtggtcgc gttctgggtc gcgctggcgc tcacgcgcaa
     5641 acgcttccgg gcctgagaac cgcttcggcg ccgcgccgtg cggccgcctt gatcatgtcg
     5701 gctgccttct ccgcgatcat gatgatcggc gcgttggtgt tgcccgcgac gatgtgcggc
     5761 atgatggacg catccaccac gcgcagcccg tccacgccac gcacgcgcag ctgcgaatcc
     5821 accacgtcga gcgcgccgct gcccatgcgg caggtgccca ccgggtgata gatcgtgtcg
     5881 gcacggtcgc ggatgaactg ctcgatctcc tcgtccgatt gcgccgcggc cgaggccgcc
     5941 aactcccggc cgccgtagtc cgccagcgcg ctctgggcca ggatgttgcg cgtgatcttg
     6001 aacccgcgca ccagacgctc gatgtcgctg cgttctccca ggaaattcgg atcgatctgc
     6061 ggagccacca tggggtcgtt gctcgccaga cgcaggctgc cgcggctgtc ggggcgcagc
     6121 aggcacacat ggcaggaata gccgaggccg aacaccgtct tgcggccatg gtccaccagt
     6181 ttcgccacga cgaaatgcag ctgcaggtcc ggcaggggca cgccggggcc gctcttgatg
     6241 aagccgcccg cttcggcgaa gttggtcgtc agcctgccgc tgcgggatcg ccgccattcg
     6301 aaaatcgacc tgaccagcgg cccgacggtg gacggccata ggccgaacag ctccgcgagt
     6361 tgcggcgcat ccaccacctg gatcacgtcg ggatggtcgt gcaggttgcg gcccacgccc
     6421 ggcatatctt gcaccaccgg aacaccgtgg gcgtgcagtt gcgcccccgg cccgatgccc
     6481 gagagcatga gcagcttcgg cgactggagg gcgcccgcgc tgagcagcac ctcgcgcgcg
     6541 gctttcagcc gtttgagctg cccttcctgg tggtactcga cccctatgac ccgtcgccct
     6601 tccgtgagaa tgcgcgtcgt gtgagcgtcg gtgatcacct tcaggttcgg tcggcccagg
     6661 ttgggcgcga cataggcctt cgcggcgctg aatcgctcgc cgttcctgtg ggtcacctga
     6721 tagagcccga agccttcctg ctgcacgccg ttgaagtccg ggttctgggg gtagcccgcc
     6781 tgtacggccg cctgcacgaa caccggcccg aagcggttgg ggctgcgcaa gtccatcaca
     6841 ttcagtgggc cgccgactcc gtggaattca tcggcccccc gctcgttgtg ctcggcccgc
     6901 ttgaaatacg gcagcacatc gtcccagccc cagccttcat tgccctcggc agcccagcgg
     6961 tcgtagtcgc tgtgatggcc gcggatgtag atcatggcat tgaccgaact cgatccgccc
     7021 agcaccttgc cgcacggctg gtagccgcgc cgcccgttca gcccgggctg cggcacggtg
     7081 ctgaacgccc agttcgcctg tcccgtcttt gcaatgagcg ccagtccggc cgggcagtgg
     7141 atcagcacgc tgcggtccgg cggtccggcc tccaggaggg caaccttgac gccggggtcc
     7201 tcgctcaggc ggccggccag cacgcagccg gcggatccgg cgccaatgat gatgtagtcg
     7261 aaatccgtgt ccatgcccgt gttcccccgg ttgccggagc accttaccgc caaaagggcg
     7321 ttcgatttgg aggctgacct ctagactcgc tacgactgcg gaacgtctcc tgcaagcgcg
     7381 atcagtccgg gaactgtcat tgcgggcttg acccgcaacc caggaagtca tggatcccgg
     7441 gtcaagcccg ggatgacagc tctcttggtg tcattgcgga cttgatccgc aatccaggac
     7501 agcccttcag tccgggatta cgctctcggg agttagattg cacgcaattt aatttgacgc
     7561 tataattcat cccatggcac acaagatcga tcccgaacag gccctgctgc tggacaacca
     7621 gctttgcttc gcgctgtatt cgacctcgct cgccatgacc aagctgtaca agccgctgct
     7681 ggacgagctc ggcctgacct atccccagta cctggccatg ctggtgctgt gggagcgtga
     7741 cggtctgatg gtctcggagc tgggcgagcg actgttcctc gattccggaa ccctcacgcc
     7801 gttgctcaag cggcttgaaa gcgccggcct ggtcgcgcgc attcgcgacg tgcaagacga
     7861 gcggcgcgtg catgtcacgc tcacggcggc cggccgcaag ctcaaggcca aggcgcaaaa
     7921 agtgccgggc tgcgtcctga gcgccagcca atgctcgatt cccgaactgg ttcagctcac
     7981 ccggcagctg cgcggcctgc gccagcgact ggccggctag ggcctccctc gcccactccc
     8041 cattttctca accgcctcca tctgaaatca acccaaagga aactacccat gagccaactc
     8101 gaaaaagtcc tctacaccgc aaaagcccac accaccggcg ggcgcgatgg cgcgtcgcgc
     8161 accgacgacg gccgcctgga catcaagctg tcgcctcccg gcatgggcgg cagcggcacc
     8221 aaccccgagc agctcttcgc ggccggctac tcggcctgct tcatcggcgc tttgaaagcg
     8281 gtcgcgggca agatgaagat gacgttgccg gccgacctgg ccgtcgacgc cgaagtcgac
     8341 ctgggccctg tcgccggcgg ctacggcatc gccgcgcgcc tgaacgtgag cctccccggc
     8401 ttcgagcgcg ccgccgcgca agccctggtg gatgctgcgc acaaggtctg cccctactcc
     8461 aacgcgaccc gcggcaacat cgacgtcaag atcaagctcg tctgaggctg cgttggccgg
     8521 acggccaggc tcgcggcccg cgtcgtccgg cccggcttga cgtgggtcaa cgagtcgtgg
     8581 cggggcccaa gccagtcagc gacggctaga ggatgatcgg agccggcacc aaccatcctc
     8641 tcccatgaaa ctcgccttgc tgtccgacct gcacgccaac cggcaggcgc tcgaggcctg
     8701 cctggccgac gcccgtgcgc gcggggcgac gcagtacgcg ttcctgggcg acgtcgtcgg
     8761 ctacggaggc gagcccgccg ccgtgctcga ccgtgtcatg agcatggccg gggatggcgc
     8821 ctgggtggtg cgcggcaacc atgacgaagc ggccataaaa cccttgccgg gcagcaggcg
     8881 cgccgatcac accggcgccg catggacccg cgcacaactg aatccagccc agcaagaatt
     8941 cctggcagcc ctgccgctga tgctgcggca ggacttcatc ttgctggtcc atgccagcgc
     9001 gcgcgagccc cagcgctggg cctatgtcga ggatgccacc ggcgcaaccg ccagcctgga
     9061 tgccgccgtt gtcgaaggcg cgacccatgt gttcggcggc catgtgcatg agcaacggtt
     9121 gttcttccag gggaccggcc gcggcgccat ggggttcgag cccaccgccg gcgtgcccat
     9181 cccgttgccc cgccaccggc gatggctggc caccgtcggc tccgtcggcc agccgcgcga
     9241 tggcaggcgc gacgccatgt atgcgctgtt cgacagcacc tccacccggc tggtcttcct
     9301 gcgcgtgcct tacgaccacg aggccgccgc gcgcgcgatc cggcaggccg gcttgcccga
     9361 gttctatgcc cagcgcctcg cgctggggcg ctaggccgcg cccgtgaagg agccgccgtg
     9421 aaacagctga atcccggaac cgagatcgac ggcttcctga tcggcgagtg cgtgcacgcc
     9481 gggggcatgg cccatgtgta ccgcgtgcgc tattccaggg cgggccacga ccccggtttc
     9541 ccgatggcga tgaaagtgcc gcgcatgacc agcggcgacg gcgcggaaaa catcgtgagc
     9601 ttcgaagtcg agcaccagat gctgcaagcg ctgtcgggcc cgcatgttcc gcgcctggtc
     9661 gccaccggcg atatggcacg gctgccttac ctggtcatgg aatacatcga aggccggacg
     9721 ctgcagcact ggctggatcg ggcgagtgag gatcgcaccc ggccgtccgc ccaggacatc
     9781 gcccggctcg gcgcggccac ggcggtcgcg gcgcacagcc tgcaccagca ggaagcggtc
     9841 catctggacc tcaagcccgg caatgtgatg atccgcccgg acggcggcgc cgtgctgctc
     9901 gatttcggcc tgagctggca tgggcactat cccgacttgc tggccgagga gatgcgctcg
     9961 gccatcggct ccccaccctg gatcgcgccg gagcaggtgg tgggcgtgcg cggcgatccg
    10021 cgcagcgatg tgttcgcgat cggcgtgatg ctgtacgagc tggcgaccgg caagctcccg
    10081 ttcggcaatc cgcaaacccg cggcggactg cgccagcgcc tgtggatgga cccgcgaccg
    10141 ccgcgcctgc tggcgccgga aaccccgtca tggctgcagg agatcatcct gcgctgcctg
    10201 gagcccgagg ccgacgaccg ctatgcctcc gccgcgcacc tggcgttcga cctggcgcac
    10261 ccggatcagg tgcaggtggg ggcgcggggc gagaaaacgc gccggacctc ctggcgcgcc
    10321 catgccaaac gctggctcaa ggcggcgggc ctgcactatc accccagccc cctgcccagc
    10381 tcgcaggtgg agcaggtgcc gatcgtgatg gtggccgtgc cgtggaagga tgcgagcgat
    10441 gccacgctct attcgctgcg gcgggcggcc agccgttcgc tcggcatccg ccccggcgcg
    10501 cgcctggctt gcgtgacggt gatcgcgccg ggcgacacgg acccgaccga cgatcgctgc
    10561 agcgaagtcc aggtgcggcg ctggcagctc gaccggctgc gccagtgggc gcagggcctg
    10621 gacctgcagg gccaccggct ctcatttcac gtcctcgagg ccggcgacac ggcgcaggcg
    10681 ctgctcgaat acgcgcgagc caaccgcgtc acgctgatga tcctgggcgc ggccacccac
    10741 ggcctgaaaa tgcagcgcct gatcgccacc gtgccggtac gcgtcgccat ggatgcgcct
    10801 tgcagcgtca tgctggtcaa gcagtcgctg ccgttcgagc ggctgggcga gaacagcgcg
    10861 accgcggacg cgccggcggc gacaatgggc catgtcgtct catccctatg aagtactgac
    10921 gcccgacgtg gtcatggacg cgctggcatt cgtcggcctg cgcggcgacg gccgcctgat
    10981 ggcgctgagc tcctacgaaa accgcgttta ccaggcccac ctggaggaac cggtggaagg
    11041 ccacggcgcg gtggtcgcca agttctaccg gccgggccgc tggagcgagg cgcagatcct
    11101 ggaagaacac gccttctcgg ccgaactcgc cgccgccgag gttccggccg tggcgccgct
    11161 tctgctgaac ggcagcaccc tgcatgagca cggcggtttc gccttcagcg tgagcccgcg
    11221 ccgcggcggc cgggcgccgg aactcgatga cgcggaagtg ctggagtgga tcggccgctt
    11281 tctcgcgcgc atccacatgg tcggcgcgag ccggcctttc gcaacccggc cggcactcga
    11341 cctgcggagt ttcgggcagg agccgcgcga ctggttactg gagcaccaca tgatcccgct
    11401 ggatgtgcag gcggtctggg agcggcgctg cgacgaggcg ctgcgcatga tcaaggacac
    11461 ggccctgggc gacggcgcgg ccggaggcat ggcgatgatc cgcctgcacg gcgattgcca
    11521 tccgggcaac atcctgtgga ccccggacca gggcccccac tttgtggacc tcgatgacgc
    11581 gcgcaccggc ccggcggtgc aggatatgtg gatgctgctg tcgggcgagc gggcgcagcg
    11641 ccagcgccag ctcggccctc tggtcgacgg ctatgagttg ttccgcgaat tcgaccgcaa
    11701 cgagctggcg ctgatcgagc cgctgcgcac gctgcggctg atccactaca gcgcctggct
    11761 ggcgcggcgc tgggacgacc cgatcttccc gatcaatttt ccctggttcg gctccagcga
    11821 ctactggcag ggccaggtgc agatgcttga ggaacagatc gaggcgatgg cggagccgcc
    11881 gctggtggtc tgaagttgtt gtcattgcgg gcttgacccg caatccaggt attcgagcat
    11941 aattttgctc actatcctta ttaatgggca taatgctgct caattttctg gccctcgatt
    12001 ccctttttac gtacaattct gctcactatc caacttaacg agcaatattt tgcccagcat
    12061 caaaactatc cccttcgagg tttcgcagtc gctggtcgtc ctgggccaac gcgtgaagaa
    12121 agcacgcatg cggcggcagc tgagccagga ggagctagcg caggcctgca agattgcccg
    12181 gcgtaccctc ttcagcattg aggccggggc tccgggcatc gccgtcggcc acatctacac
    12241 cgtgctctgg aagcttggcc tgctcgccac aacgcgcgga gttgcagacc ctgatgcgga
    12301 tgagcacgga aaaatccttg aagcggcaaa actaggcaag cgagttcgcc ggccgcaaga
    12361 caaggctgaa gacaacaact tctgagtacg aacattccaa tggctacgcc cgaccagttc
    12421 tgctacgtct acatccagtt gccgggggcc tttgattggg tcccctgcgc ctccctgcgg
    12481 atcagggagg ttgccg
//
LOCUS       DASWIY010000271         4774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3486230, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000271 DASWIY010000000
VERSION     DASWIY010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3486230"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VGA59_10050"
     CDS             <1..428
                     /locus_tag="VGA59_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX9720061.1"
                     /translation="LSSDFIVGFPGETEEDFGKLMKLVQDVGYDSSFSFIFSPRPGTP
                     AATLHDETPHEVKLKRLQRLQTVLDDNVRAISASRQGTVQRVLVEGPSHKDPAVFAGR
                     TDCNRLVLFAGQPRLAGRMVDVRITEASQRSLRGEVVVP"
     gene            complement(542..1906)
                     /gene="ffh"
                     /locus_tag="VGA59_10055"
     CDS             complement(542..1906)
                     /gene="ffh"
                     /locus_tag="VGA59_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX9720062.1"
                     /translation="MASALSDKLSRLAKEIRGQARITEANVQDMLREVRMALLEADVA
                     LPVVRDFIARVKDKAMGQEVLGSLSPGQALVGIVNRELAATMGQGVSDINLQAQPPAV
                     ILMAGLQGAGKTTTTAKLAKHLIEKRKKKVLTVSGDVYRPAAIEQLKTVTKQAGAEWF
                     PSSPEQKPVEIARAALDYARKQYFDVLLVDTAGRLAIDEVLMREIKELHAALNPVETL
                     FVVDAMQGQDAVNTAKAFKEALPLTGIILTKMDGDSRGGAALSVRQVTGAPIKFAGVS
                     EKIDGLEVFDADRHAGRILGMGDIVALVEQVTAGVDMQAAQKLAAKVKSGAGFDLNDF
                     LSQIQQMKSMGGLSSLIDKLPAQMAAKAGEVDMNKAEREIRRKEGIIQSMTPMERRKP
                     ELIKATRKRRIAAGAGVHVQEVNRLLNEFEQMQGMMKKMKGGGLMKMMKRMGGGKGMP
                     KMPF"
     gene            2017..2820
                     /gene="ccsA"
                     /locus_tag="VGA59_10060"
     CDS             2017..2820
                     /gene="ccsA"
                     /locus_tag="VGA59_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX9720063.1"
                     /translation="MILASASPASVVLTVAAAVAYAVPAAAASRLSEKAARMALLAAW
                     LLHAFVLAAGLLGSPPRFGFAPALSVTAWLVLTVYAVERQLFPHLQGRWALAGLGSAA
                     VLLTLIFPGSPLPATASPWLPLHWALGIASYGLFAAAVVHAWMMTRAEKQIRQAADPH
                     AGVPLLTLERLTFRFAAAGFVLLSATLLAGLLFSDALYGAGRGFKWDHKTVFSVLSWL
                     VFAFLLLGRFRFGWRGRKAVRVLYAGSLLLLLAYAGSRFVIEVVLRRPL"
     gene            2817..3062
                     /locus_tag="VGA59_10065"
     CDS             2817..3062
                     /locus_tag="VGA59_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEX9720064.1"
                     /translation="MKYLVLLAVVLAVLWFLRNSRRGGPSRGPPRDRTSDRASAASPQ
                     DMVECPVCLVHLPRSDALPGPDGQFYCCTEHRARAGD"
     gene            3064..>4774
                     /locus_tag="VGA59_10070"
     CDS             3064..>4774
                     /locus_tag="VGA59_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9720065.1"
                     /translation="MPALPSEFPLAPEPPGLTSDSAFARLWRGFMMARIAIALVLLLL
                     LAALYMLVPGINISPWLIGLCAVYLVAALAVRMFTRPSLPGNTFDPQWVSTIGVDLVA
                     VSTLQFLQTAGINYTPLFALSVLMASVLGSGLLALGTAAGVALLLLGDAWLASLAVSA
                     DTAARFLQAGLTGSGYFALAFLANQLAARLAREESAARRGRRAARIQAQVNELVIEAL
                     VDGILVVDPHGRVHAANPAARTLLGFGQAGGNTPFELSDQPEWTPVMELARLTFAQRQ
                     PQFGDVALEEGGAGARRLHVRTRLTPAHDHPAERLCVMFLEDLREMEARLRTEKLAAM
                     GRMSAAVAHEIRNPLAAIAQANALMEEDLREPALKQLSFLVRKNAQRLSQIVDEILDI
                     SRVQHQGLAGAAVLELDTTVWAACGDWMMQNGSGDRLQLTPCAPAAQVAFETDHLRRV
                     LVNLLDNALRYAGHEPDSICVSTHAATGQAGLQVWSDGAPLEPAVRRHLFEPFFSSES
                     RSSGLGLYICRELCERHGAAIGYRRAAGGCAGNPREGNEFFVSFRQVPGPLAGTASFD
                     TIAA"
BASE COUNT          643 a         1642 c         1593 g          896 t
ORIGIN      
        1 ccctgtccag cgacttcatc gtgggcttcc cgggcgagac ggaagaagac ttcggcaagc
       61 tgatgaagct ggtccaggac gtgggctacg acagctcgtt cagcttcatc ttcagtcccc
      121 ggcccggcac gccggccgcc accctgcatg acgaaacacc gcacgaagtg aagctcaagc
      181 gcctgcagcg gctgcaaacc gtgctcgacg acaatgtgcg agcgatcagc gccagccgcc
      241 agggcacggt acagcgcgtg ctggtggaag ggccgtcgca caaggacccg gccgtcttcg
      301 cgggccgcac cgactgcaac cggctggtgc tgttcgccgg gcagccccgc ctcgcggggc
      361 ggatggtgga cgtgcgcatc accgaggcga gccagcggtc gttgcgcggc gaggtggttg
      421 tgccttgatc agttggggac agagcaaaat tgctgcgcaa ttcttgattt gacccacaag
      481 ctctgatcag ttggggacag agcaaaattg ctgcgcaatt cttgatctgt cccacaaggt
      541 ctcagaaagg catcttgggc atgcccttgc cgccgcccat gcgtttcatc atcttcatca
      601 ggccgccgcc cttcatcttc ttcatcatgc cctgcatctg ctcgaactcg ttcaggaggc
      661 gattgacttc ctgcacgtga acgccggcgc cggcggcgat acggcgcttg cgggtggctt
      721 tgatcagttc gggtttgcgc cgctccatcg gcgtcatgct ctggatgatg ccttccttgc
      781 gccggatttc gcgctcggcc ttgttcatgt cgacctcgcc ggccttggcc gccatctggg
      841 ccggcagctt gtcgatcagg ctcgacagcc cgcccatgct cttcatctgc tggatctgcg
      901 acaggaaatc gttcaggtcg aagccggcac cgctcttgac cttggccgcg agcttttgcg
      961 ccgcctgcat gtcgaccccg gccgtgacct gctcgaccag ggccacgatg tcgcccatgc
     1021 ccaggatgcg gcccgcgtgg cggtcagcat cgaacacttc cagcccgtcg atcttctcgc
     1081 tgacgccggc gaacttgatc ggcgcgccgg tgacctgccg caccgacaat gccgcgccgc
     1141 cgcgcgagtc gccgtccatc ttggtgagga tgatgccggt cagcggcaag gcttccttga
     1201 aggccttggc ggtgttgacc gcgtcctggc cctgcatggc gtcgaccacg aacagggtct
     1261 cgaccgggtt caaagccgcg tggagctcct tgatttcgcg catcaggact tcgtcgatcg
     1321 ccagccgccc cgcggtatcc accaggagca cgtcgaaata ctgtttgcgc gcatagtcca
     1381 gggccgccct ggcaatttcc accggcttct gctcggggga gctggggaac cattcggcgc
     1441 cggcctgctt cgtcacggtc ttgagctgct cgatggcggc cggacggtac acgtcgcccg
     1501 acaccgtcag caccttcttc ttgcgcttct cgatcaggtg cttggccagt ttggccgtgg
     1561 tggtggtctt gcccgcgccc tgcaggcccg ccatcaggat caccgccggc ggttgcgcct
     1621 gcaggttgat gtcgctgacc ccctgcccca tggtggccgc gagttcgcgg ttcacgatcc
     1681 ccaccagagc ctggcccgga gagagcgacc ccagcacttc ctggcccatc gccttgtcct
     1741 tcacccgggc gatgaagtcg cgcaccaccg gcaatgccac gtcggcttcg agcagcgcca
     1801 tgcggacctc gcgcagcatg tcctgcacgt tggcttcggt gatgcgggcc tggccgcgga
     1861 tttccttggc gaggcgggaa agtttgtcgg agagcgcgga ggccatggtg gcgttaaggg
     1921 ttcggcgtca ggcgttgggc gtgaaaaaga cgcgtttgga gtgaatcgct caacgctcga
     1981 cgctgaacgc ccaaccagcc tttaaactgt gaacccatga ttctagccag tgcgtccccc
     2041 gcgagtgtgg tgctgaccgt ggccgccgcg gtggcctatg ccgttcccgc cgcggccgcc
     2101 tcccgcctct cggagaaagc ggcgcgcatg gccctgctgg ccgcgtggct gctgcacgct
     2161 ttcgtgctgg ccgcgggcct gctcggctcg ccgccgcgct tcggcttcgc gccggcgctg
     2221 tcggtcaccg cctggctggt gctcaccgtc tacgcggtcg agcggcagct ctttccccat
     2281 ctgcaagggc gctgggcgct ggccggcctg ggttccgccg ccgtgctgct cacgctgata
     2341 ttccccggca gcccgctgcc ggcgaccgcg tcaccgtggc tgcccctgca ttgggccctg
     2401 ggcatcgcct cctacggcct gttcgccgcc gcggtcgtcc atgcatggat gatgacccgc
     2461 gccgaaaagc agatccgtca ggcggccgac ccgcacgcgg gcgtgccgct gctgacgctc
     2521 gagcgcctga cgttccgctt cgcggccgcc ggcttcgtcc tgctctcggc cacgctgctg
     2581 gccggcctgc tgttcagcga tgcgctctac ggggcgggcc gcggcttcaa gtgggaccac
     2641 aagaccgtgt tctccgtgct gtcctggctg gtattcgcgt tcctgctgct gggacgcttt
     2701 cgcttcggct ggcgcggccg caaggcggtg cgggtgctgt acgccggatc gctgctgctg
     2761 ctgctggcgt acgcgggttc gcgtttcgtg atcgaggtgg tgctcaggcg gcccctatga
     2821 agtatctggt gctgttggcg gtcgtgctgg ccgtcctctg gttcttgcgc aacagccggc
     2881 gcggcggccc ctcccgcggg ccgccgcgcg atcgcacgag cgaccgcgcg tcggcggcaa
     2941 gtccgcagga catggtggaa tgcccggtct gtctggtgca cctgccgcgc agcgacgcgt
     3001 tgcccggccc ggatgggcag ttctactgct gcaccgaaca ccgcgcgcgt gccggggatt
     3061 gacatgccgg cgctgccgtc cgaattccct cttgcgcccg agccgcccgg gctgacctcg
     3121 gactcggcct tcgcgcggct ttggcgcggg ttcatgatgg cccgcatcgc catcgcgctg
     3181 gtgctgttgc tgctgctcgc ggcactttac atgcttgtcc cggggatcaa catcagcccc
     3241 tggctgatcg ggctttgtgc cgtgtacctg gtggcggcgc tggcggtgcg catgttcacc
     3301 cggccctcgc tgcccggcaa tactttcgat ccgcagtggg tatccaccat cggtgtcgac
     3361 ctggtggctg tttcgaccct gcagttcctg caaaccgccg gcatcaatta cacgccgctt
     3421 ttcgctttgt ccgtgctgat ggcctcggtg ctgggctcgg ggctgctggc gctgggcacg
     3481 gcggccggcg tggccctgct gctgctgggc gacgcctggc tggcatccct ggccgtctcg
     3541 gccgacaccg cggcacgctt cctccaggcg ggcctgaccg gcagcggcta tttcgccctg
     3601 gccttcctgg ccaaccagtt ggccgcccgt cttgcgcgcg aggaaagcgc ggcccggcgc
     3661 ggccggcgcg cggcccgcat ccaggcccag gtcaacgaac tcgtgatcga ggcgctggtc
     3721 gacggcatcc tggtggtcga tccgcacggc cgggtgcatg ccgccaatcc ggcggcacgc
     3781 accctgctgg gcttcgggca ggcgggcggc aacacgccgt tcgagttgtc ggaccaacct
     3841 gagtggacgc ctgtcatgga gcttgcgcga ctgactttcg cgcagcgcca gccccagttc
     3901 ggcgatgtcg cgctcgagga gggcggcgcg ggtgcgcgcc gccttcatgt ccgcacgcgg
     3961 ctgacgcccg cccacgatca tccggccgag agactttgcg tgatgttcct ggaagacctg
     4021 cgcgaaatgg aggcgcggct gcgcaccgaa aagctggccg cgatgggccg gatgtcggcg
     4081 gccgtggcgc acgaaatccg caatccgctg gcggcgatcg cccaggccaa cgccctgatg
     4141 gaggaggacc tgcgcgagcc cgcactcaag cagctgagtt ttctggtacg caaaaatgcc
     4201 cagcggctgt cccagatcgt ggacgaaatc ctggatatct cgcgcgtgca gcaccagggc
     4261 ctggcggggg ccgccgtgct cgaactggac acgaccgtgt gggccgcctg cggcgactgg
     4321 atgatgcaga acggcagcgg cgaccggctg cagctcacgc cgtgcgcgcc ggccgcgcag
     4381 gtggcgttcg agacggacca cctgcgccgg gtgctggtga acctgctgga caacgcattg
     4441 cgctacgcgg gccacgagcc cgattcgatc tgcgtctcga cgcatgcggc caccggccag
     4501 gccggcctgc aggtctggag cgacggcgcg ccgctggagc cggccgtgcg gcgccacctg
     4561 ttcgagccgt tcttctcctc agagagccgc tccagcggcc tgggcttgta tatctgccgc
     4621 gaactgtgcg agcggcacgg ggccgcgatc ggctaccgcc gcgcggcggg cggctgcgcc
     4681 ggcaacccgc gcgagggcaa cgagttcttc gtcagtttcc gccaggttcc cggcccgctt
     4741 gcggggaccg cttcatttga cacaatagct gcct
//
LOCUS       DASWIY010000272         6827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1667475, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000272 DASWIY010000000
VERSION     DASWIY010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667475"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..737
                     /locus_tag="VGA59_10075"
     CDS             192..737
                     /locus_tag="VGA59_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HEX9720066.1"
                     /translation="MKRLNALLAAALIGVSLAAGAHQGATLGKKAGAVVKEQKAWGVA
                     GDAKAVKRTIEVVMTDNMRFTPDRVEVKEGETIRFVHRNAGKIMHEFVIGTRKELDQH
                     AAMMLKFPNMEHDEPYMAHVAAGKTGEIIWTFNRPGEFEFACLIPGHYQAGMDGKIKV
                     VPGGEGLAKARKETGTAARRS"
     gene            796..1134
                     /locus_tag="VGA59_10080"
     CDS             796..1134
                     /locus_tag="VGA59_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEX9720067.1"
                     /translation="MNAFKLSLLAGAIGMAGAFTLPAQAQTAAAGAAAAATASDMADA
                     EVRKVDKENRKITLKHGAIKNLDMPPMTMVFQVADASMLDKLQPGDKIRFRATSDAGK
                     MTVIEIQPVK"
     gene            1164..1625
                     /locus_tag="VGA59_10085"
     CDS             1164..1625
                     /locus_tag="VGA59_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9720068.1"
                     /translation="MLPWLIAGAVLLLVAAIWLAPRGERSSIDPRDKALVATGKRIYD
                     THCGSCHGANLEGQPNWRERLPNGRLPAPPHDAGGHTWHHPDAVLFGIVKEGLAPGKY
                     APPQYESDMPAFGGSLSDEEIRSVLAYIKSTWPERELAYQRQMDIGSRERH"
     gene            complement(1721..3577)
                     /gene="dsbD"
                     /locus_tag="VGA59_10090"
     CDS             complement(1721..3577)
                     /gene="dsbD"
                     /locus_tag="VGA59_10090"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEX9720069.1"
                     /translation="MFTSPPPPQSSPALPSAGPLDSVRSWLRGPAPPAELLPPEQAFQ
                     LAVQARDAETLIAQLTPAKDYYLYRDRISFSVQEPPGIMVAGVRFPKGKPKADPTFGT
                     VEVFHDPFDVVIRLQRSGEPADRLTLRASYQGCNEPLGVCYPPIEKTISVALSNAVAS
                     GAPSQAAQETPGGASESSAPPRSDSSEDGSFRRLFTGGSTWALVAAFFGFGLLLAFTP
                     CMLPMIPILSGIIVGQGHTMTRRHALGLSAVYVLGMAITYAAAGVAAGLAGTLLAAYL
                     QTPWVLGSFAAIFVLLALSMFGFYELQLPTSLQSRLASAGSRVKGGRVAGAFLMGVLS
                     AVIVGPCVAAPLAGALLYIGQTGDTIFGGLALFSMAVGMGVPLLVVGATAGALLPKAG
                     PWMVSVKRLFGVMMLALAIYLVSPVIPLVAQQLLWAVLLIVSAVFLHAIDPLPHDAPG
                     HRRLFKGVGVIGLAGGVALLIGALSGSKDLLQPLGGLGGAPVEVRRELAFVPVKSVAD
                     LDARLQAARGRPVMLDFWAEWCVSCKEMDRFTFSDAQVQARLKNVLLLRADVTANSAD
                     DQALLRRFSLFGPPGIVFFDTRGTELAYRVIGFEPPQKFLASLDRVLAPAAP"
     gene            complement(3715..5367)
                     /locus_tag="VGA59_10095"
     CDS             complement(3715..5367)
                     /locus_tag="VGA59_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX9720070.1"
                     /translation="MLNDSFGGRRRMLIGSAGAAALAALGAPALAARAATEAPATADV
                     HIELEAVADQVSVRPGAPTRVWRYRGRLLRGEAGALEAPAGTFVGPIIRVRRGQRVRI
                     ELVNRLPESTIIHWHGLHVPEAMDGHPRFAIAPGERYVYDFTVANRAGSYWFHPHPHG
                     RTGKQVYFGLAGLFLVSDEEEARLGLPTGAYDLPLIIQDRNFDADNQLVYLSGGVIGE
                     AASAPRGPGMMGGGMMGGMMGGSRGGMMGGMGAMMAGMMGVLGEQILVNGRPDAGIEV
                     ARRPHRLRLFNGSNTRMYKLAWSDRSPLTVIGTDGGLLAAPLQRDYVMLAPAERIDLW
                     VDFARWPGGPELSLRSLAFEGGMDMGGMMGQTALRDGAPFTVLKLRVARGGEARARLP
                     AKLADVPPPDPVLAVNADHPKVFRLTMGMMTWGINDHGFDMLGASELETVKLGTHEVW
                     EFRNDASSGMMGMTMPHSMHVHGVQFRVIGRSIARRFERDRDTVKAGLVDEGWKDTVL
                     AMPGERVRVLLHFADYTGLFLYHCHMLEREDSGLMRNYLVKA"
     gene            complement(5357..6262)
                     /locus_tag="VGA59_10100"
     CDS             complement(5357..6262)
                     /locus_tag="VGA59_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX9720071.1"
                     /translation="MLFSLRPIFAIASTLLLATLAAIPAHATVTLTHVHGLSYSADGK
                     QLLIPSHHGLAVFADGRWTMAAGPAHDYMGFSATRDALYSSGHPAPGSGLTNPFGLVK
                     SRDGGKTWQGLGLEGESDFHTLATSYGTNAVYVVNHQPNSRMSHPGIYYTLADGLKWQ
                     RAEARGLRTKLNALAVHPADAKVVAAGADDGLYLSRDSAASFSPLVNRTRVLAQTFDL
                     DGQHLWFSAYSGKATLAKIALKPGAKPENRPLPALANDAVAYIAQNPARRDEMAIATF
                     NRSVFISQDEGRSWKQIAKEGSGAE"
     gene            complement(6322..>6827)
                     /locus_tag="VGA59_10105"
     CDS             complement(6322..>6827)
                     /locus_tag="VGA59_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX9720072.1"
                     /translation="STDISVVEWGRDFRLLDHHGNSRSIADFKGEVVLLFFGFTHCPD
                     LCPTTMADMARAVASMGPDGARVQGLFATVDPQRDTQQVLAKYVTAFDPSFLGLYGDE
                     ATTAALAREFKFFHGTEPGHSHDNYSVTHGGSIYVYDPQGRLRLLMGPGWTVDTMLAD
                     VRLLLKE"
BASE COUNT         1264 a         2263 c         2237 g         1063 t
ORIGIN      
        1 atacgagttc caggggcggc tgcccgaggc gccgcgtgca gccgcctctg gaaccggctc
       61 catgccggcc aaggccgcgc ccaggaaaga cgtcgaagtt cagatacgca aaccctccgg
      121 ccattccggc cattccgacc attgatgcgg cgagacatcg ccacccattc acattaccga
      181 aggatcgata gatgaaaaga ctgaatgcct tgctggctgc cgcgctgatc ggcgtatcgc
      241 tcgccgccgg cgcgcaccaa ggggcaaccc tcggcaagaa agccggcgcc gtcgtgaaag
      301 agcagaaggc ctggggcgtt gccggcgacg cgaaggccgt gaagcggacc attgaagtcg
      361 tcatgaccga taacatgcgt ttcacgcccg accgcgtcga ggtcaaggag ggcgagacga
      421 tccggttcgt ccacaggaat gccggcaaga tcatgcacga gttcgtgatc ggtacccgca
      481 aggagctgga ccaacacgcc gccatgatgc tcaagttccc gaacatggag cacgacgagc
      541 cttacatggc gcacgtcgcg gcgggaaaga caggcgagat catctggacc ttcaatcgcc
      601 cgggcgagtt cgaattcgcg tgcctgatcc ccggtcacta ccaggccggc atggacggca
      661 agatcaaggt tgttcccggc ggcgaaggcc tggcgaaggc tcgcaaggag acgggcaccg
      721 cagcccggcg aagctgaacg ttccggctgg atcggccaat gaaacctcac atcagaagca
      781 gaaaggaaat atgacatgaa tgcattcaag ctgtcacttc tcgccggtgc gatcggcatg
      841 gccggcgcgt tcaccctgcc ggcgcaggcc caaaccgcag cggccggggc cgccgccgcc
      901 gcaaccgcca gcgacatggc cgatgcggag gtgcgcaagg tggacaagga gaacaggaag
      961 atcaccctga agcacggcgc catcaagaac ctggatatgc cgcccatgac gatggtgttc
     1021 caggtggcgg acgcgtccat gctggacaag ctccagcccg gtgacaagat ccgcttcagg
     1081 gccacgagcg acgccggcaa aatgacagtg atcgagatac agcctgtgaa gtgaccgtac
     1141 agcgtcgtca tgtcccccgc cgcatgcttc cttggctgat cgccggcgcc gtgcttctcc
     1201 tggtggctgc gatttggttg gccccgaggg gggagcgctc gagcattgat ccgcgggaca
     1261 aggcgctggt ggccaccggc aagcgcatct acgacaccca ctgcggctcg tgtcacggtg
     1321 cgaatctgga ggggcaaccc aactggcgcg agcgactgcc caacgggcgc ctgcccgctc
     1381 cgccgcacga cgccggtggc catacctggc atcatccgga cgccgtgctc ttcggcatcg
     1441 tcaaggaagg cctggcgccg ggcaaatacg caccgccgca atacgaaagc gacatgccgg
     1501 catttggcgg gagcctgtcc gacgaggaga ttcgatcggt gctcgcttac atcaaaagca
     1561 cttggcccga acgggagctg gcctatcaac ggcagatgga catcggcagt cgcgagcggc
     1621 attgagtcgt tgaggggtgg cgcacccgcc cctcggctcc ttgcgactgc ggccaaacgg
     1681 ccgaacgttt tccggcgaat cctgccgcaa cggaaagccc ttacggcgcg gcgggcgcca
     1741 gcactcggtc cagcgaagcg aggaacttct gcggcggctc gaagccgatc acccggtagg
     1801 cgagctccgt tccccgggtg tcgaaaaaca cgatgcctgg cggtccgaac agcgaaaagc
     1861 ggcgcagcaa agcctggtcg tcggcgctgt tggctgtcac gtccgcgcgc aacaggagca
     1921 catttttcag ccgcgcctgc acttgcgcat cgctgaacgt gaagcggtcc atttccttgc
     1981 aggacacgca ccactccgcc cagaagtcga gcatcacggg gcgtccgcgc gcggcctgca
     2041 atcgcgcgtc gaggtcagcc acgcttttca ccggcacgaa cgccagctcg cgccgcacct
     2101 cgacgggtgc gccacccaga ccgcccagcg gctgcagcag gtccttgctg cccgacagcg
     2161 caccgatcag cagcgccacg ccacccgcca gcccgatcac gcccacgccc ttgaacagcc
     2221 gccggtggcc cggtgcatcg tgcggcaacg gatcgatggc gtgcaagaac acggcggaca
     2281 cgatcagcag caccgcccac agcagctgct gggcgacgag cggaatcacc ggcgagacga
     2341 gatagatcgc cagcgcgagc atcatgacgc cgaacaagcg cttgaccgac accatccacg
     2401 ggccggcctt ggggagcaag gcgcccgccg tggcgcccac caccagcagc ggcacaccca
     2461 tgccaacggc catcgagaac agcgccaacc cgccaaagat cgtgtcgcct gtctggccga
     2521 tgtagagcag ggcgcccgcc agcggcgcgg ctacgcacgg gccgacgatc acggcagaga
     2581 gcacacccat caggaaggcg ccggccaccc ggccgccctt gacgcggctg ccggcggaag
     2641 ccagtctgct ctgcagcgaa gtcggcagtt gcagttcgta gaaaccgaac atcgacaacg
     2701 cgagcagcac gaagatcgcg gcgaagctgc ccagtaccca tggagtctgc aagtaagccg
     2761 ccagcagcgt gccggccaag ccggccgcca cgccggccgc cgcgtaggtg atggccatcc
     2821 ccagcacgta gaccgccgac aggccgagcg catgccgtcg ggtcatggtg tggccctggc
     2881 cgacgatgat gcccgacagg atgggaatca tcggcagcat gcagggcgtg aaggcgagca
     2941 gcagcccgaa gccgaagaag gccgccacca atgcccaggt gctgccgccg gtgaacagac
     3001 ggcgaaagct cccatcctcg gaggagtcgc tgcgcggcgg ggcggatgac tcgcttgctc
     3061 cgccaggcgt ttcctgcgct gcttgggaag gtgcgccact cgcaacggca ttggacagcg
     3121 caacgctgat cgtcttctcg atcggcggat agcacacgcc gagcggttcg ttgcagccct
     3181 ggtagctggc gcgcaaggtg agacggtctg cgggctcgcc cgatcgctga agacggatga
     3241 ccacgtcgaa cggatcgtgg aacacctcca cggtcccgaa ggtcggatcg gccttgggct
     3301 tgcccttggg gaatcgaacg ccggcgacca tgatcccggg cggctcctgc acgctgaagc
     3361 tgatgcggtc acggtagagg taatagtcct tggccggagt cagttgcgca atcaaagttt
     3421 cggcgtcccg ggcttgcacc gccagctgga acgcctgctc cggcggcagc agttcggccg
     3481 gcggcgcggg tccacgtaac cagcttcgca ccgaatccag cgggccggcg gacggcagtg
     3541 ccggcgacga ttgcggcggc ggtggactgg tgaacaagtt gccagcttgc ggatatgcga
     3601 cagcgcccgc agccagcagg agcagcccga cgactgcgcg cgcaagcgaa acgctgtggc
     3661 agcggacctc tctgcccacg gcagatcgcg cggcaccgac atgttttccg gcgttcatgc
     3721 cttcaccaga tagttgcgca tcagcccgga gtcttcgcgt tccagcatgt ggcagtggta
     3781 gaggaacaga cccgtgtaat ccgcgaagtg cagcaggacg cgcacgcgtt ctccgggcat
     3841 cgcgagcacg gtgtctttcc atccctcgtc cacgaggccg gccttcaccg tatcgcgatc
     3901 gcgctcgaat cgccttgcaa tcgaacgccc gatcacccgg aactgcaccc cgtgcacatg
     3961 catcgagtgg ggcatggtca tccccatcat cccggacgag gcgtcgttgc ggaactccca
     4021 gacttcgtgt gtgcccagct tgaccgtttc gagctcgctt gcgcccagca tgtcgaagcc
     4081 gtgatcgttg atgccccagg tcatcatgcc catggtcagc ctgaacacct tcgggtgatc
     4141 ggcgttgacg gccagaaccg gatcgggtgg cggcacgtca gccagcttgg cgggcaacct
     4201 ggcgcgggcc tcgccgcccc gcgcgacccg cagcttcaac accgtgaagg gcgcgccgtc
     4261 gcgcagtgcg gtctggccca tcatgccgcc catgtccatg ccgccctcga aggcgaggct
     4321 tcgcaagctc agttccggcc cgccgggcca gcgggcgaag tccacccaca ggtcgattcg
     4381 ctcggccggc gccagcatga cgtagtcgcg ttgcaggggc gcggcgagca gtccgccgtc
     4441 ggtgccgatc acggtgagcg gcgagcggtc gctccaggcg agtttgtaca tgcgcgtgtt
     4501 ggagccattg aaaagccgca gccgatgcgg ccggcgcgcc acctcgatgc ctgcatccgg
     4561 ccggccgttg acgaggatct gctcgccgag cacgcccatc atgccggcca tcatcgcgcc
     4621 catgccgccc atcatgccgc cgcggcttcc tcccatcatg ccgcccatca tgcctccacc
     4681 catcattccg ggcccgcgcg gagcgcttgc ggcttcgccg atcacaccgc ctgacaagta
     4741 gaccaactga ttgtcggcgt caaagttgcg gtcctggatg atcagcggaa gatcgtaggc
     4801 gcccgtgggc aagccgagac gcgcttcctc ctcatcgctg accagaaaca ggcccgccag
     4861 accaaagtac acctgcttgc cggtacggcc atgcggatgc ggatgaaacc agtagctgcc
     4921 ggcgcggttg gcgacggtga agtcgtagac atagcgctcg ccgggcgcga tcgcaaagcg
     4981 cgggtggccg tccatggcct cgggcacgtg caggccatgc caatggatga tggtggactc
     5041 cggcaggcga ttgaccagtt cgatgcgcac ccgttggccc cgccgcacgc ggatgatcgg
     5101 tccgacaaac gtgcccgccg gcgcctcgag cgcgccggcc tccccgcgca gcagcctgcc
     5161 acggtagcgc catacgcggg tgggtgctcc ggggcgaacg ctaacctggt ccgcaaccgc
     5221 ctcgagttcg atgtgaacat cggcggtggc cggtgcttcg gtagcggcgc gggcggccag
     5281 ggccggtgcc cccagcgctg ccagtgccgc cgcgccggcc gagccgatca gcattcggcg
     5341 gcggccgccg aacgagtcat tcagcaccgc tgccctcctt ggcgatctgt ttccagctgc
     5401 gcccctcgtc ctggctgatg aacacgcttc ggttgaatgt cgcgattgcc atctcgtccc
     5461 gccgcgccgg gttctgtgcg atatacgcca cggcgtcgtt cgcgagcgcg ggcaacggac
     5521 ggttctccgg tttggcgccg ggcttcaagg caattttcgc cagcgtggct ttgcccgaat
     5581 aggcgctgaa ccagagatgt tgtccatcca ggtcgaaggt ctgcgcgagc acccgagtcc
     5641 tgttgacgag cggcgaaaag ctggcggcgg aatccctgga caggtagagc ccgtcgtccg
     5701 cgccggccgc gaccaccttg gcgtcggcgg gatgcaccgc cagggcattg agcttggtgc
     5761 gcaggccacg cgcctctgcg cgctgccact tgaggccgtc ggccagcgtg taataaatgc
     5821 cgggatggct catgcgcgag ttcggctggt ggttgaccac gtagaccgcg ttggtgccgt
     5881 aactcgtcgc caacgtgtgg aagtccgact ctccctccag gccgagccct tgccatgtct
     5941 tgccgccgtc gcggctcttg accaggccga acggattggt cagccccgat ccgggtgccg
     6001 gatggccgct gctgtagagc gcatcccgcg tggcggagaa gcccatgtaa tcgtgcgcag
     6061 gtcccgcggc catggtccag cgtccatcgg cgaacacggc cagcccatgg tgggagggga
     6121 tcaggagctg cttgccatcg gcactgtagg acagcccgtg aacatgggtc agtgtgactg
     6181 tggcgtgagc tggtattgct gccagcgtgg cgagcagcag tgtcgaggcg atagcaaaaa
     6241 tgggtcggag cgaaaaaagc atgttgaacc ttggtgttgg ttggatgcgg tgtcgatagc
     6301 gtgaaagtgg gcgtggcaat ctcattcctt cagcagcagc ctgacgtcgg ccagcatcgt
     6361 gtcgacggtc catcccggcc ccatcagcag gcgcagccgg ccttgcggat cgtagacata
     6421 gatggagccg ccgtgggtaa cggagtagtt gtcgtgcgaa tggcccggct cggtgccgtg
     6481 gaagaacttg aactcccggg ccagcgccgc ggtcgtggct tcatcgccgt acaagccgag
     6541 gaagctcgga tcaaacgccg tgacgtactt tgcgagcacc tgctgcgtgt cgcgctgtgg
     6601 atccaccgtc gcgaacagcc cctggacccg cgcgccgtcc gggcccatgc tggcaacggc
     6661 gcgggccatg tcggccatcg tcgtcgggca gaggtccggg cagtgggtga agccgaagaa
     6721 cagcagcact acctcgccct tgaagtcggc gatgctccgg ctgtttccgt gatggtccag
     6781 cagacgaaag tcgcgtcccc actcgacaac cgaaatgtcc gtggact
//
LOCUS       DASWIY010000273         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2413846, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000273 DASWIY010000000
VERSION     DASWIY010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2413846"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VGA59_10110"
     CDS             <1..249
                     /locus_tag="VGA59_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9720073.1"
                     /translation="KVGPTEFKLLHYLMKHPERVHSRSQLLDKVWGDHVFIEERTVDV
                     HVKRLRESLGAAGAMIETVRGAGYRLAAHSAAASGAAG"
     gene            292..1671
                     /gene="phoR"
                     /locus_tag="VGA59_10115"
     CDS             292..1671
                     /gene="phoR"
                     /locus_tag="VGA59_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEX9720074.1"
                     /translation="MAWRFFSFLCCELAGGLIGWWLGHDYAIRTGLVAGGLGWLAVDS
                     LRGARVLQWLRRGDFMAVPSVSGAWGEIVERARRVLRARDQELVDARQRLQEFLAAIQ
                     GSPNGVILLDAQGRIEWCNETAADQFGLDPRRDLMQQVTNLVRDPSFTAYYNDVGSSG
                     GRDIVIPGPRSVPSRPVKLSLYLHSYGEGRRLLLSRDVTAVEQAEVMRRDFIANVSHE
                     IRTPLTVLMGFVETLQNLPLDAEERGRYLDMMARQAQRMRTLVGDLLTLSRLEGSPLP
                     GAGEWTPVAMLLAQCEQDGKALSDLLVKPLNIRFGAPPSLEIGAAPSELLSAMSNLVS
                     NAVRYTPAGGSVDVQWRALPDGRGEFSVRDTGPGIAPEHIPRLTERFYRVDRSRSRET
                     GGTGLGLAIVKHVVQRHGAELSIDSTPGEGSTFAIRFPASRLRPLAPVSPGPQVDTGA
                     PPARESLAG"
     gene            complement(1610..3022)
                     /locus_tag="VGA59_10120"
     CDS             complement(1610..3022)
                     /locus_tag="VGA59_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9720075.1"
                     /translation="MSELRTIARHAGTVLVGQLAVMAFGVADTIVAGRYSQEALAALS
                     VGSAVYISVFVGLLGVLQALLPTWAELHGRSNIAPADSHPLPLGEGRGEGTRHEDVGR
                     SVRQALYLGALCMLAGGSALLFPGPLLRWTEVPVQLQAEVTRYLAVLALALPPALLFR
                     MYSTLNQSLGRPMLVTWLQAMSLLVKVPLTIWFAFGGLGLPAQGAVGCAWATVAVNYL
                     FLGCAVWLLRTQPLYRPYAIWRPLERPDWRAIGQFARLGVPGGLAVMVEVTSFTLMAL
                     FIARQGTLASAAHQIAANLAAVLYMMPLSIAIATSARVSFWLGADNASRARLAIRTGF
                     KLGLLMALAVAASLAMGSGRIPAIYSRSPEVGAVAAGLLAWLALYHVGDAAQVLCVFV
                     LRCYRVAVAPLIAYCVLLWGVGLLGGYALAYRGLGPWNAAHSPAAFWAAGAVALALLS
                     LILPAILWRAVRRYRPAAQG"
     gene            complement(3019..>4192)
                     /locus_tag="VGA59_10125"
     CDS             complement(3019..>4192)
                     /locus_tag="VGA59_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720076.1"
                     /translation="ALAQVAFTSLGFYAMAALPRRPAMAGLWLFMGMAGLALSGAPAM
                     AMLFSAGGLVISLLDGDARSDASRTTPWIAAAILAAALLATMVLAWWLDLWRWRISPE
                     RDWRALGRLLLWFTWPAWPLALWTIWRWRRQLGSRHVALPLWFVLVSVVTTAVTPSSD
                     RSLLLGLPALATLAAFALPTLGRSVAALIDWFTLLFFSGCAFVIWVIWVSMQTGVPAK
                     PAANVAKLAPGFEPSFSALAFVAAVAATLAWAWLVKWRTGRHRAVIWKSLVLPAGGAA
                     LSWLLLMTLWLPVLDFARSYTPVVNAVMRHIDRPGCVEVFGLTRAQLAAFRYHGNLEM
                     RPAGWQGACHWLLVASALQPSMASALDTSGWQLVSIIRRPVDKDDNVLLYRKADSR"
BASE COUNT          740 a         1455 c         1443 g          554 t
ORIGIN      
        1 aaagtgggtc ccaccgagtt caagctgctg cactacctga tgaagcatcc cgagcgggtg
       61 cacagtcgtt cgcagctgct ggacaaagtc tggggcgacc atgtgttcat cgaggaacgc
      121 acggtcgatg tccatgtgaa gcgtctgcgc gagtctctgg gggcggccgg cgccatgatc
      181 gagaccgtgc gtggcgcggg ctaccggctg gcggcccaca gcgccgccgc cagcggggcg
      241 gccggctagg gcggcgctcg gtccgcggtg cccgccgggt cacaatcagc catggcgtgg
      301 cgctttttca gcttcctgtg ttgcgaactg gccggcggcc tgatcggctg gtggttggga
      361 cacgactacg ccatacggac cgggctggtg gccggtggcc tgggttggct ggccgtggac
      421 agcttgcgcg gtgcgcgcgt gctccagtgg ctgcggcgcg gggatttcat ggcagtcccg
      481 tccgtatccg gtgcctgggg tgaaatcgtg gagcgcgcgc ggcgcgtcct gcgcgcgcgc
      541 gatcaggaac tcgtggatgc ccgtcagcgg ctgcaggagt tcctggcggc gatccagggt
      601 tcgcccaatg gcgtgatcct gctggacgcc cagggccgca tcgaatggtg caacgagacg
      661 gcggccgacc agttcgggct cgacccgcgg cgcgacctga tgcagcaggt gaccaatctg
      721 gtccgcgatc cctcgttcac ggcctattac aacgacgtcg gcagcagcgg cgggcgggac
      781 atcgtcattc ccggcccgcg cagcgtgccc tcgcgaccgg tcaagctgtc gctgtatctg
      841 cattcctatg gcgaggggcg caggctgctg ctctcgcgtg acgtgacggc ggtggagcag
      901 gccgaagtga tgcgccgaga cttcatcgcc aacgtttcgc atgagatccg cacgccactc
      961 accgtcctca tgggtttcgt ggagacactc caaaaccttc cgctggacgc ggaagaacgc
     1021 ggccgctacc tggacatgat ggcccggcag gcgcagcgga tgcggacgct ggtcggcgat
     1081 ctgctgaccc tgtcgaggct ggaaggcagc ccgttgcccg gtgccggcga gtggacgccg
     1141 gtcgccatgc tgctggcgca gtgcgaacag gacggcaagg cgctttcgga cctgctggtc
     1201 aagccgctga acattcgttt cggcgcgccg ccgtcactgg aaatcggggc ggcgccttcg
     1261 gaactgctca gcgccatgtc caacctcgtg agcaatgccg tacgctatac gccggccgga
     1321 ggttccgtcg atgtgcagtg gcgggccttg cccgatggcc gcggtgaatt ttcggtgcgc
     1381 gataccggcc ccggcatcgc gcccgagcac attccgcgtc tcaccgagcg tttctaccgc
     1441 gtcgatcgaa gccggtcgcg cgaaaccggc ggcaccgggt tgggcctggc catcgtcaag
     1501 cacgtggtgc agcgtcacgg cgccgagttg tcgatcgaca gcacgccggg cgaggggtcg
     1561 accttcgcca tccggtttcc ggccagccgc ttgcgccccc tcgcgccggt cagccctggg
     1621 ccgcaggtcg ataccggcgc accgcccgcc agagaatcgc tggcaggatg aggctgagca
     1681 gggccagcgc cacggcgccg gccgcccaga aggccgcggg cgagtgcgcc gcgttccatg
     1741 ggcccaggcc ccggtaagcc agcgcatagc cgcccagcag tccaacgccc cacagcagca
     1801 cgcagtacgc gatcagcggt gccacggcga cgcggtagca gcgcagcacg aacacgcaaa
     1861 gtacctgggc cgcatctccc acatggtaga gcgcgagcca ggccagcagc ccggccgcca
     1921 ccgcccccac ctccgggctg cgtgaataga tcgcggggat gcgcccgctg cccattgcca
     1981 ggcttgcggc caccgcgagc gccatcagca ggccgagctt gaagcccgtg cggatggcca
     2041 gccgcgcgcg gctcgcgttg tccgcgccca gccagaagct cacgcgtgcg ctggtcgcga
     2101 tggcgatcga caaaggcatc atgtagagga cggccgccag gttcgcggcg atctggtgcg
     2161 cagccgaagc gagcgtgccc tggcgcgcaa tgaacagcgc catcagggtg aaggacgtca
     2221 cctccaccat gacggcaagc ccgccgggca cgcccaggcg ggcgaactgg cctatggccc
     2281 tccagtcggg ccgctccagc ggccgccaga tcgcgtaggg gcgatagagc ggctgggtgc
     2341 gcagcagcca cacggcgcaa cccagaaaaa gatagttcac cgcgaccgtc gcccaggcac
     2401 agccgaccgc accctgcgcc ggcaggccca gcccgccgaa cgcgaaccag atcgtcaacg
     2461 gcactttcac gagcagcgac atcgcctgca gccaggtcac gagcatgggc ctgcccaggc
     2521 tctggttcag tgtgctgtac atgcgaaaca gcagggccgg cggcagcgcc agcgccagca
     2581 cggccaggta gcgggtgacc tcggcctgca attgcaccgg gacctcggtc cagcgcagca
     2641 gcgggccggg aaacagcagg gccgagccgc cggccagcat gcagagcgcg cccaggtaca
     2701 gtgcttgccg aacggaccgg ccgacatcct cgtgacgagt gccctcaccc cggccctctc
     2761 ccagagggag agggtgagaa tcggctggcg cgatattcga cctgccgtgc agttcggccc
     2821 aggtgggcag caaagcctgc agcacgccca acaggcccac gaagacgctg atatacaccg
     2881 ccgagccaac cgacagcgcg gccagcgcct cctgagaata ccggcccgcc acgatggtgt
     2941 ccgccacgcc gaaggccatc accgcaagct ggcccaccag caccgttccc gcatgccggg
     3001 cgatggtgcg caactcgctc accgcgaatc ggccttccgg taaagcagca cgttgtcgtc
     3061 tttgtcgacc gggcgccgga tgatggagac aagctgccaa ccactcgtgt ccagcgctga
     3121 cgccatcgac ggctggagtg ccgaggccac cagcagccag tggcaggcac cctgccagcc
     3181 ggcgggtcgc atttccagat tgccgtgata gcggaatgcc gccaactgcg cgcgggtgag
     3241 accgaaaact tcgacacagc cgggccggtc gatatggcgc atgacagcat tcaccaccgg
     3301 cgtatagctg cgcgcgaagt ccagcaccgg cagccacagc gtcatcaaca gcagccagga
     3361 cagggcggcc ccgccggcgg gcagcaccag gctcttccag atgaccgcgc gatggcggcc
     3421 ggtccgccac ttcaccagcc aggcccaggc gagcgtggcg gccaccgccg ccacgaaggc
     3481 cagcgccgag aagctgggct cgaagccggg cgcgagcttg gccacgttgg cggccggctt
     3541 ggccggcaca ccggtctgca tggacaccca gatcacccag atgacgaacg cgcagccgct
     3601 gaagaacagc agtgtgaacc agtcgatcaa cgcggccacg ctgcgaccca gcgtgggcag
     3661 cgcaaaagcg gccagcgtgg ccaatgccgg caacccgagc agcagcgaac ggtccgacga
     3721 gggggtgacc gctgtggtca cgaccgacac cagcacgaac cacagcggca gcgcgacgtg
     3781 acggctgccg agctggcggc gccagcgcca gatcgtccac aaggccagcg gccacgcggg
     3841 ccaggtgaac cacagcagca gccgtccgag cgcgcgccag tcgcgctccg gactgatgcg
     3901 ccagcgccag aggtccagcc accaggccag aaccatggtg gcgagcaggg ccgcggccag
     3961 gatggcggcg gcgatccagg gcgtggtgcg cgaggcgtcg ctgcgggcgt cgccgtcaag
     4021 caggctgatc accagtcctc cagcgctgaa cagcatcgcc atggccggcg cgccgctcag
     4081 cgccagtccc gccatgccca tgaacagcca gagccccgcc atggccggcc ggcggggaag
     4141 cgccgccatg gcgtagaagc cgagggaagt gaaggccacc tgggccaacg cc
//
LOCUS       DASWIY010000274         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2416254, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000274 DASWIY010000000
VERSION     DASWIY010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2416254"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VGA59_10130"
     CDS             complement(<1..836)
                     /locus_tag="VGA59_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9720077.1"
                     /translation="MTAAVAAASFAATVRHRTHWTDRIAHAALLVIAALFMVALVAPL
                     WTILAQSVEDRSGQFVGLANFVSYLRTPALASSLWNSSWVSAVVTLAVIPTAFACAYA
                     LTRSCMPAKGLFRAITLIPLLAPSLLSAISFIYWFGNQGLLKSWLSALGITSIYGAPG
                     IILSEVFSVFPHALMILVTALTLADARLYEAADAMGTSATRQFFTITLPGAKYGLISA
                     SLVTFTLVITDFGIPKVIGGNFNMLATDVFKLVIGQQDFQRGAVVALFLLAPALLTFA
                     VD"
     gene            complement(833..1966)
                     /locus_tag="VGA59_10135"
     CDS             complement(833..1966)
                     /locus_tag="VGA59_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX9720078.1"
                     /translation="MTTTRTDFLVLKDISKNFGGFQALKNVEFGVAKGEFVCFLGPSG
                     CGKTTLLRIIAGLEVQTAGSILQAGSDISRLPPAQRDYGIVFQSYALFPNLSVADNIA
                     YGLVNRKTPKARIDARVAELLKLIGLPGSERKYPAQLSGGQQQRIALARALATSPSLL
                     LLDEPLSALDALERVRLRGEIRALQRTLGVTTIMVTHDQEEALSMADRIVVMNQGIIE
                     QIGSAIEVYREPASPFVADFVGRVNVLTATVDGPGRVRIGGRIFACAHAVGETGRAVK
                     AYLRPEDVLARPLEPGDPNVLDGRIEKIEFLGSYCLVRVVVDGMESQLLTVCLSLNLL
                     SEQNLAEGSRLKLRVLTERLRFFSDHASSLPHAQAPLEPGAVA"
     gene            complement(2039..>2665)
                     /locus_tag="VGA59_10140"
     CDS             complement(2039..>2665)
                     /locus_tag="VGA59_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9720079.1"
                     /translation="FNTVEAAKRKIPKPGTWKDLTKAAYKGQIVMPNPASSGTGYFDV
                     TAWLQIWGEAEGWKFMDGLHQNIAQYTHSGSKPCNMAAAGEFVVGISFEYRANSNKAK
                     GAPIDLIFPKEGLGWDLEAFAIHKGTKKLDAARKLADWASSKEAMILYGANFAITAQP
                     GVAKPLPNVPADYEARLVKNDFTWAANNRERILAEWTKRYDAKTEPKK"
BASE COUNT          516 a          814 c          850 g          485 t
ORIGIN      
        1 tgatccaccg cgaacgtcag cagcgccggc gccagcagaa acagcgcgac gaccgcgcct
       61 ctctggaagt cctgctgacc gatgaccagc ttgaagacgt cggtggcgag catgttgaaa
      121 ttgccgccga tcactttggg aatgccgaag tcggtgatca ccagggtgaa agtgaccagg
      181 ctcgcgctga tgaggccgta tttcgcgccc ggcagcgtaa tggtgaaaaa ctggcgcgtc
      241 gcgctcgtcc ccatcgcatc cgcggcttca tagagacggg cgtccgccag cgtcagcgcg
      301 gtcaccagga tcatcaaagc gtgtggaaac acgctgaaca cctcggacag aatgatgccc
      361 ggcgcgccgt agatcgaggt gatgcccagt gccgacagcc agctcttgag cagtccctgg
      421 ttgccgaacc agtaaatgaa cgaaatcgcc gacagcagag acggcgcaag cagcgggatc
      481 agcgtgatcg cgcgaaacag gcccttggcc ggcatgcaac tgcgcgtgag cgcataggcg
      541 caggcgaagg ctgtggggat cacggccagc gtcacgaccg ccgataccca ggaactgttc
      601 cacagcgagc tggcgagtgc cggtgtgcgc aggtaggaaa cgaagttcgc cagaccgacg
      661 aactggccgg acctgtcctc gacgctctgc gcgagaatgg tccagagcgg cgccaccagc
      721 gccaccatga acaacgcggc gatgaccagc agcgcggcgt gcgcgatgcg atcagtccag
      781 tgggtacggt gccgaacggt ggccgcgaat gatgccgccg caacagcggc cgtcacgcca
      841 ctgctcccgg ttcaagtggt gcctgcgcat gcgggaggga agaagcgtgg tcggagaaaa
      901 aacgcagcct ttcggtgagc acccgcagct tgaggcggct gccctcggcg aggttctgtt
      961 ccgacagaag attcaacgac aggcagaccg tcagcagttg cgattccatg ccgtcgacga
     1021 cgacgcgcac caggcaatat gagccgagga actcgatttt ttcgatgcgg ccgtccagca
     1081 cgttcggatc gccgggctcg agtggccgcg ccagcacatc ctcgggccgc agatacgcct
     1141 tgactgcacg ccccgtctcg ccaacggcgt gagcacaggc aaagatgcgc ccgccaatac
     1201 gcacgcggcc tggaccgtca accgtcgccg tcaggacgtt gaccctgccg acgaaatccg
     1261 ccacgaacgg tgacgccggt tcgcggtaga cctcgatcgc agagccgatc tgctcgatga
     1321 tcccttggtt catcacgacg atgcggtcgg ccattgacag cgcttcttcc tgatcgtgcg
     1381 tcaccatgat ggttgtgacg ccgagcgtgc gttgcagcgc gcggatctcc ccgcgcaggc
     1441 gcacccgctc cagcgcgtcg agcgcggaca gcggctcgtc gagcagcagc aggctgggcg
     1501 atgttgccag cgcgcgcgca agcgcgatgc gctgttgttg cccgccggag agctgggccg
     1561 gatacttgcg ctcgctgccg ggcaggccga tcaacttgag caattccgcg acgcgggcgt
     1621 cgatcctggc cttcggcgtc ttgcggttga ccaagccgta cgcgatattg tcggcgacgc
     1681 tgagatttgg aaacagcgcg tacgactgga acacaatgcc gtagtcgcgc tgcgccggcg
     1741 gcagccgtga gatgtcactg ccggcctgaa ggatgctgcc ggcggtctgc acttcgaggc
     1801 cggcgatgat gcgcagcagg gtggtcttgc cgcacccgga cggtccgaga aaacagacaa
     1861 actcgccttt cgccacccca aactcgacgt ttttcagcgc ctgaaagccg ccgaagttct
     1921 tgctgatgtc tttgagcacc agaaagtcgg tgcgggtggt cgtcatgcgt ataaaaacca
     1981 aaaacaaaac ccgaaggcgc cagggcacct tcgggttgaa cgttccacaa ttccgtattt
     2041 acttcttggg ctcagtcttg gcgtcgtagc gcttggtcca ctcggcgagt atgcgctcac
     2101 ggttattggc ggcccaggtg aaatcgtttt tcaccaaacg cgcctcatag tccgccggta
     2161 cgttgggcag cggcttggca acgccgggct gagccgtgat cgcaaagttt gcgccgtaga
     2221 ggatcatggc ttccttgctc gaagcccagt cggcaagctt tctggccgca tccagcttct
     2281 tggtgccttt gtggatggca aacgcctcga gatcccaacc cagtccttct ttcgggaaga
     2341 tgaggtcgat cggcgcgcct ttggccttat tggagttggc gcggtactcg aacgaaatgc
     2401 cgacgacgaa ctcgcccgcg gctgccatgt tgcacggctt ggagccggaa tgcgtgtatt
     2461 gggcgatgtt ctggtgcagg ccatccatga atttccagcc ttcagcctcg ccccagatct
     2521 gcagccaggc ggtgacatcg aaatagccgg tgcccgatga ggccgggttg ggcatcacga
     2581 tctggccttt gtaggctgcc ttggtcaggt ccttccaggt tccgggcttg gggattttgc
     2641 gcttggcggc ttccacagtg ttgaa
//
LOCUS       DASWIY010000275         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3490614, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000275 DASWIY010000000
VERSION     DASWIY010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3490614"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VGA59_10145"
     CDS             complement(<1..786)
                     /locus_tag="VGA59_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720080.1"
                     /translation="MEGSPKGAGLECASTHSARAVSRPPRLFADAPDFVIEARQLGKC
                     YQLYERPADRLKQLVWGGRLRRGKPYYRDFWALKDVDFAIAPGEIVGIVGRNGAGKST
                     LLQLVCGTLHATTGQLSVRGRVAALLELGAGFNPDFTGCENIYMNAAIMGLGRAQVDE
                     RFDEIVAFADIGDFITQPVKTYSSGMYMRLAFAVATCVEPDILVIDEALSVGDGSFAR
                     KSFDRIMALKEAGKTILFCSHSMAQVEALCSRAMWIDAGHLRLA"
     gene            865..1896
                     /locus_tag="VGA59_10150"
     CDS             865..1896
                     /locus_tag="VGA59_10150"
                     /inference="COORDINATES: protein motif:HMM:NF023701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HEX9720081.1"
                     /translation="MMPSSKLLYRPHELAFRTQYAELKERARSTAAMLPGTPGTLVKR
                     TGTGRAYWYRVYRTASGAQVEDLVCKDGDEEALRNARAGLDFAQWTAAQVRNLRKLDF
                     QVADKGVASVLVELHNAGLLAAGVVVVGTLGYMAWLNELGARAVSARTQDIDLAARQR
                     LKLAAPQSFLQTVQATRLGFVPVPGLPNKAQATSVKLSGRDGLRVDVLAHGEHLGATV
                     PVPQLMWHARTVPHYDYLLSRPREAAILAGGHCIPVWLPQPERLVWHKLYSSASRQSF
                     PEKADKDLLQAGTLAAVLTEQEDQPLSDSLDDVPPAMKQVLRKRLPAARRVLSGHDRA
                     LEALEQALA"
BASE COUNT          426 a          707 c          700 g          369 t
ORIGIN      
        1 ggcgaggcgc aggtggcctg cgtcgatcca catcgcgcgc gagcacagcg cctccacctg
       61 ggccatggaa tgggagcaga acaaaatggt tttgcccgct tccttcaggg ccatgatgcg
      121 atcgaaggac ttgcgggcga aagagccgtc tcccacggaa agcgcttcgt cgatcaccag
      181 gatgtcgggc tccacgcagg tcgccacggc gaatgccagt cgcatgtaca tgccgctgga
      241 ataggtcttg accggctggg tgatgaagtc gccgatgtct gcgaacgcca caatctcgtc
      301 gaatcgctcg tcgacctgcg cgcgacccag acccatgatc gcagcgttca tgtagatgtt
      361 ctcgcagccg gtgaaatctg gattgaaccc ggcaccgagc tccagaaggg ccgccacgcg
      421 gccgcggacg ctcagctggc ccgtcgtggc atgcagggtg ccgcatacca actgcagcaa
      481 agtcgacttg cccgctccgt tgcgccctac gatgcccacg atctctcccg gggcgatcgc
      541 aaagtccacg tccttcaacg cccagaaatc ccggtaataa ggctttccgc gccgcagccg
      601 gccgccccac accaactgct tgagccggtc agccggacgt tcgtacagct ggtagcattt
      661 tccgagctgg cgcgcttcaa tcacgaaatc gggggcatcg gcaaacagtc gaggaggacg
      721 ggacacggcg cgggctgagt gagttgacgc gcattctaag cccgcaccct tgggcgaacc
      781 ttccacgccg gggcgcctga tcgtgaaacc atgaatcatg gtcgccttgc atacccagta
      841 aaatcgtaaa ctcctgggta cgccatgatg ccatcgtcaa agctgctcta ccgaccgcac
      901 gaactggcct tccgcacgca gtacgccgag ctgaaggagc gggcccgctc gaccgcggct
      961 atgctgcccg gaacgcccgg cacgctggtc aagcgaacgg gcaccggtcg cgcctactgg
     1021 tatcgggtgt accggacggc ttccggcgcc caggtcgaag acctggtgtg caaggacggc
     1081 gatgaagagg cgctgcgcaa cgcccgtgcc ggactcgact tcgcgcaatg gacggctgcc
     1141 caggttcgca acttgcgcaa gctcgatttc caggtggccg acaaaggcgt tgcgagcgtc
     1201 ctggtggagt tgcacaacgc gggcctgctg gccgcgggcg tggttgtcgt ggggacgctg
     1261 ggctacatgg cctggctgaa cgagttgggg gcaagggcgg tctcggcccg cacccaggac
     1321 atcgacctgg ccgcacgcca gcgcctgaag ctcgcggcgc cgcagtcgtt cctgcagacg
     1381 gtgcaagcca ccaggcttgg attcgtgcct gtgcctgggc tgcccaacaa ggcacaggcc
     1441 acctcggtca agctcagcgg acgcgacggg ctgcgggtgg acgttctcgc gcacggagag
     1501 cacctgggag cgacggtacc cgtgccacag ctgatgtggc atgcccggac ggtgccccac
     1561 tacgactact tgctcagccg gcccagggag gcggccatcc ttgccggcgg acattgcatc
     1621 ccggtatggc tgccgcaacc ggagcggctc gtttggcaca agctctattc gagcgcgtca
     1681 cgccagagct ttccggagaa agccgacaag gacctgttgc aagcaggcac cctggcggct
     1741 gtgctgacgg agcaggaaga ccagccactg tccgactctc tggacgacgt gccgcccgcc
     1801 atgaagcaag tactgcgcaa gcgactgccc gccgcacgcc gggtgttgag cgggcatgat
     1861 cgagcgctgg aggcgctcga acaggcactt gcgtaggagc atgacaacgc ttcataggct
     1921 gtcattgcgg acctgatccg caatccaggc tttcgcggct gcgtggatgc cgggtcaagc
     1981 ccggcatgac aacttacgct ctgcctcgcg gactccgccg cttcacgggc aaaagcgaaa
     2041 ggccgatcgg gccgctcagg tatcgaatat tcatatagga ctgctgtaca ctcccgttcc
     2101 tatatgaaaa aaccgctgcg cgtcaccttg agcacatcgc ccgagcaggc caagggattg
     2161 atggcgttgc aaaaggcatt cgcgcaggtc tgcaacgcgt tg
//
LOCUS       DASWIY010000276         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3151410, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000276 DASWIY010000000
VERSION     DASWIY010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3151410"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..>2342
                     /locus_tag="VGA59_10155"
     CDS             12..>2342
                     /locus_tag="VGA59_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9720082.1"
                     /translation="MRDWLAAKRDRAAALLASRKQAKRLKADAMLEAAHAVFALLPEQ
                     GCEGLAGLTAAHRQELHRDIGKKTAGWSEADFAEAAGLIQIARQALAVDGSLTRDLLA
                     LLCPFAADVRKTFLSQGWVSFDGLLARARALLREHPGIRERLKHEYQAVLVDEFQDTD
                     PVQYEIILYLAERAGRQGRDWRTIDLDPGKLFIVGDPKQSIYAFRRADIEAFERVVEK
                     VAASGGITYALATNFRSDERVLEVVNPLFDRLLVRQDHVQPANVPLIVRPNRRSRIGQ
                     PGVEVRVVRAEEDEEVTSTAATRHEAEQLARWIKDELLANETLADASGGRHELKPGHI
                     ALLFRKLTQAQEYLDALRRHEIAYITDGEKHFYRRQEVIDLVNVLRVVENPHDAIALA
                     GVLRSPLGGVTDRELVELRARGAFDYRQAARLSGWASARAEAVQLLYGRLADLHRAAP
                     SVPLPEAMDRLFDRLPVLELAAASLHGEQAVANLFKVRQMAVELAGRPHLTWTAFVEL
                     MITRLDEQPVEAESALAEESLEAVRVLTIHKAKGLEFPVVILPGAHHGIGRGRELPPV
                     SHDWASGVFGINWADHSSLGAVLVNEKARLREEAERRRLFYVGMTRAKERLILSGGWP
                     SRPSKGTFLDLLREAATGEIGEAAVPTLHIGPVPVAQAVLTAHERAPRRRRQAPSKLQ
                     ASGEWAAVADRWTQRDRTWEAVRAAPIHLTPTGLTKPGERGPGEPAARAAGPAARSRL
                     VGTLAHRVLQHWDFAQPPDRLQKRISALCETDLPSEL"
BASE COUNT          379 a          729 c          885 g          349 t
ORIGIN      
        1 cggcgcccgc gttgcgcgac tggctggccg cgaagcgcga ccgcgccgcg gcactcctgg
       61 cctcgcgaaa acaggcgaaa cgcctcaagg cggacgccat gctggaggcg gcgcacgcgg
      121 tgtttgccct actgccggag cagggctgcg aggggctggc ggggctgacg gccgcgcacc
      181 ggcaggaact gcaccgggac atcggcaaga aaacggcggg ctggagcgag gccgactttg
      241 cggaggcagc gggcctgatc cagatcgcca ggcaggcgct cgcagtggac gggtcgctca
      301 cgcgggacct gctcgcgctg ctctgcccgt tcgccgcgga cgtccgcaag acgtttctct
      361 cccaggggtg ggtgtcgttc gacgggctgc tggcgcgggc gcgcgccctg ttgcgcgagc
      421 atcccgggat ccgggagcgg ctgaagcacg agtatcaggc ggtcctggtg gacgagttcc
      481 aggacacgga ccctgtgcag tacgaaatca tcctctatct ggccgagcgg gcggggcggc
      541 agggacgcga ctggcgcacg atcgatctgg atccgggcaa gctgttcatc gtgggcgatc
      601 ccaagcaatc catctacgcg ttccgacggg ccgacatcga agcgttcgag cgggtggtcg
      661 aaaaggtcgc cgcgtccggc gggatcacct atgcgctggc cacgaatttt cgcagcgacg
      721 aacgggtcct ggaggtggtg aatccgttgt tcgaccggct gctggtccgg caggatcacg
      781 tgcagcccgc caacgtgccg ctgatcgtgc ggcccaaccg ccgaagccgg atcgggcagc
      841 caggcgtgga agtgcgcgtg gtgagggccg aggaggacga ggaggtgacg tcgactgcgg
      901 cgacccggca cgaagcggaa cagctcgcgc gctggatcaa ggacgagttg ctggcgaacg
      961 agacgctcgc cgacgcctcc ggaggccggc acgaactgaa gccggggcat atcgccctgt
     1021 tgttcagaaa gctgacgcag gcgcaggaat atctcgatgc gctgcgccgg cacgagatcg
     1081 cctacatcac ggacggggag aagcattttt accggcggca ggaagtcatc gacctcgtca
     1141 acgtcctgcg tgtggtggag aacccgcacg atgccattgc gttggccggc gtgcttcgtt
     1201 ctccattggg cggcgtgacg gaccgggaac tggtcgaact gcgcgcgcgc ggcgcctttg
     1261 actaccggca ggcggcgcgc ctgtccggat gggcaagcgc ccgggccgag gccgtgcaac
     1321 tactgtacgg ccgcctggcc gacctgcaca gggcggcccc gtcggtcccg ctgcccgagg
     1381 cgatggaccg gctcttcgac cggctgccgg ttctggaact ggcggcggcc tcgctgcatg
     1441 gcgagcaggc ggtcgccaat ctgttcaagg ttcggcagat ggccgtggag ctggccggga
     1501 ggccgcatct gacgtggacg gcgtttgtgg agttgatgat cacgcggctt gacgaacagc
     1561 cggtggaggc ggaaagcgcg ctggcggagg aatcgctgga ggcggtgcgc gtgctgacga
     1621 ttcacaaggc caaggggctc gagtttccgg tggtcatcct gccgggggcg catcatggca
     1681 tcggccgggg gcgtgaactg ccgccggtgt cccacgactg ggcgagcggc gtgttcggga
     1741 tcaactgggc cgaccacagc agtctcggcg ccgtgttggt gaacgagaag gcgcggctgc
     1801 gggaggaggc cgagcggcgg cgcctgttct acgtgggcat gacgcgcgcg aaggagcggt
     1861 tgattctgtc cggcggctgg cccagccgcc cttcgaaggg gacgttcctg gatctgctgc
     1921 gggaagccgc gaccggcgag atcggagagg cggctgtgcc cacgctgcac atcggcccgg
     1981 ttccagtggc gcaggcagtg ctgacggcgc atgaacgggc acccaggcgt cgcaggcagg
     2041 cgccatccaa actgcaggcg tcgggtgaat gggcggccgt ggcagaccga tggacgcagc
     2101 gcgatcgcac ctgggaagcc gtgcgcgcgg ccccgattca tctgacgccg accggcctca
     2161 cgaagccggg cgaacgggga cctggggagc cggccgcgcg ggcggctggc ccggctgcgc
     2221 gaagccgcct ggtcggcacc ctggcccacc gtgtgctgca gcactgggat ttcgcccagc
     2281 cgccggaccg tctccaaaaa cgaatttcag ccctatgcga gacggatctt ccgtctgaac
     2341 tg
//
LOCUS       DASWIY010000277         3849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1282160, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000277 DASWIY010000000
VERSION     DASWIY010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282160"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..1782
                     /gene="glnA"
                     /locus_tag="VGA59_10160"
     CDS             364..1782
                     /gene="glnA"
                     /locus_tag="VGA59_10160"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX9720083.1"
                     /translation="MAAKTVADVMKMVKENEVKFIDFRFTDTRGKEQHVSVPLSAFDE
                     DKFTSGHAFDGSSIAGWKGIEASDMLLMPDPATANIDPFYEEPTLMLSCDVLEPGDGK
                     PYERDPRSLAKRAEAYLKSSGLGDAAYFGPEPEFFVFDGVRWGVDMSGSFSKIESEEA
                     AWNSSKEYEHGNSGHRPTVKGGYFPVPPVDSFQDMRSEMCLILESLGVPVEVHHHEVA
                     TAGQMELGTKFSTLVQRADWLQIQKYVIQNVAHAYGKTATFMPKPIVGDNGSGMHVHQ
                     SVFKDGKNLFAGDGYAGLSEFALYYIGGIIKHARALNAITNPGTNSYKRLVPGFEAPV
                     KLAYSSRNRSASVRIPYVANPKGRRIEVRFPDPQANPYLAFSALLMAGLDGVENKIHP
                     GEAATKDLYHLPPEEDAKIPTVCHSLDQALDYLDKERSFLTKGGVFTDGYIDAYIELK
                     MQEVTRFRMTTHPVEFDMYYSL"
     gene            1918..2421
                     /locus_tag="VGA59_10165"
     CDS             1918..2421
                     /locus_tag="VGA59_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720084.1"
                     /translation="MNRVVSRGWWAMTLVLGLVPAAGWAQGEGKTVYRCPGNPVLYTD
                     ALSAKEAKDKGCRTLEGAPVTVVQSIKPRPPVAASGPAGSKVPPNDQRARDSDARRIL
                     ESELRREEEQLAQLQKEYNNGEPERQGDERNYQRYLDRVADMKAGIARKESDIAAIKR
                     ELAKLPL"
     gene            2442..3527
                     /gene="glnL"
                     /locus_tag="VGA59_10170"
     CDS             2442..3527
                     /gene="glnL"
                     /locus_tag="VGA59_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEX9720085.1"
                     /translation="MPGARHGSGGPAGYTALDHLATMVAIVQPDGRCVFVNAAFESVL
                     GLSRRSVLRGSLFDWFVDSQPLRDTVAAVVRNEYATSRLEAQLRRTASAHDALPVHVI
                     VNQMDRSSNVVVELVELEQQTRQDREERALDQAQANKELIRNLAHEIKNPLGGIRGAA
                     QLLEMEVESRALTEYTQVIIQEADRLQALVDRLLAPHRKPHVVSDVNIHEVCERVRSL
                     ILAEFPRGLQVARDYDISIPDFRGDREQLIQAVLNIAHNAAQALAERVALGDACITLR
                     TRIARQVTLGKQRYRLALDLHIEDNGPGISESIRDRIFYPLVSGRDGGSGLGLTLAQT
                     FVQQHQGMIECDSVPGRTNFKIVIPLP"
     gene            3570..>3849
                     /locus_tag="VGA59_10175"
     CDS             3570..>3849
                     /locus_tag="VGA59_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9720086.1"
                     /translation="MKPIWIVDDDQSIRFVLEKALAREEFAVRSFTNPRDVLAALDEE
                     APQVLVSDIRMPGGSGIELLSKVKERHPGLPVIIMTAYSDLDSAVSAFQ"
BASE COUNT          725 a         1277 c         1226 g          621 t
ORIGIN      
        1 gtcaagggcg atccggcgca actggtcaat gcctacccgg ccttgctgca aggcctggag
       61 gaacatgggg tgaagctggg ccctgaactg gtgcggtagt ccacccggga tcgactcaca
      121 tcggtgcggt tgtacgcact gttttgaggc gccgctcacc gtcgcgatct cctggaggcg
      181 caatccgtgc ccaatcgcga gcaagacggg gcgaactcgg ggcaattggg ggctcgcgaa
      241 ggccggctat cggcatggtt tctgctacat tcctgtgcgc atttttgagg caccgcggcg
      301 tgccttgccg atgttgtctc acccccccac tcacccaaac aagcgtcccg ctaggagatt
      361 ttgatggcag ccaagaccgt cgccgacgtg atgaagatgg tgaaggaaaa cgaggtcaag
      421 ttcatcgact tccgtttcac cgacacccgc ggcaaagagc agcacgtttc cgtgcccctg
      481 tccgcgttcg acgaagacaa gttcacgtcg ggccatgcct tcgatggttc gtccatcgcc
      541 ggctggaagg gcatcgaagc ctccgacatg ctgctgatgc cggacccggc caccgccaac
      601 atcgacccgt tctacgaaga acccacgctg atgctcagct gcgacgtgct cgagccgggc
      661 gacggcaagc cctacgagcg tgacccgcga tcgctcgcca agcgcgccga ggcctacctc
      721 aagtcctccg gcctgggcga tgccgcctac ttcggaccgg agcccgagtt tttcgtgttc
      781 gacggcgtgc gctggggcgt ggacatgtcc ggttcgttct ccaagatcga atccgaagaa
      841 gccgcctgga acagcagcaa ggaatacgaa cacggcaatt ccggccaccg ccccaccgta
      901 aagggtggct atttcccggt gccgccggtc gattcgttcc aggacatgcg ctcggagatg
      961 tgcctgatcc tcgaatcgct gggcgtgccg gttgaagtgc accaccacga agtggccacc
     1021 gccggccaga tggaactggg caccaagttc agcacgctgg tgcagcgcgc ggactggctg
     1081 cagatccaga agtacgtgat ccagaacgtc gcccatgcct acggcaagac cgcgaccttc
     1141 atgcccaagc ccatcgtcgg cgacaacggc tcgggcatgc acgtgcacca gtcggtgttc
     1201 aaggacggca agaacctgtt cgccggcgac ggctatgcag gcctgtcgga gttcgcgctg
     1261 tactacatcg gcggcatcat caagcatgcc cgcgcgctca acgccatcac caaccctggc
     1321 accaactcgt acaagcgcct ggtgcccggc ttcgaagccc cggtcaagct ggcctacagc
     1381 tcgcgcaacc gttcggcgtc ggtccgcatt ccctacgtgg ccaaccccaa gggccggcgt
     1441 atcgaggtgc gtttccccga tccccaggcc aacccctacc tggccttctc ggccctgctg
     1501 atggccggcc tggacggggt ggaaaacaag atccacccgg gcgaagccgc caccaaggac
     1561 ctgtaccacc tgccgccgga agaggacgcg aagatcccga ctgtctgcca cagcctggac
     1621 caggcgctgg actacctcga caaggagcgc tcgttcctga ccaagggcgg cgtgttcacc
     1681 gatggctaca tcgacgccta catcgaactc aagatgcagg aagtgacccg cttccgcatg
     1741 accacccacc cggtcgagtt cgacatgtac tacagcctgt aaaacagctc atcctgcagg
     1801 taaaggggcg gctttggccg ccccttttta cttgaagcgg gaaaccatgc atgcgtgaca
     1861 atgctcccag tcatcgtgcg ggcgccgcag cgcgttgatc ttcccaggag tgcgtgcatg
     1921 aaccgtgttg tttcgagggg ctggtgggcc atgaccctgg tgctgggtct ggtaccggct
     1981 gccgggtggg cccagggcga gggcaagacc gtctaccgct gccccggcaa tcccgtgctt
     2041 tacaccgacg cgctgtcggc caaggaggcc aaggacaagg gctgccgcac gctcgaaggg
     2101 gcgccggtca cggtggtgca gtccatcaag ccgcgtccgc ccgtcgctgc ctcggggccg
     2161 gccggcagca aggttccgcc caatgaccag cgcgcccgcg acagcgacgc gcgccgcatt
     2221 ctcgaatccg aactgcggcg cgaagaggag cagctcgcgc agttgcagaa ggaatacaac
     2281 aacggcgaac ccgaacggca gggcgacgag cgcaactacc agaggtacct cgaccgcgtg
     2341 gccgacatga aggccggcat cgcccgcaag gaaagcgaca tcgcggccat caagcgcgaa
     2401 ctggccaagc tgccgctgtg aaggtacagc ccgcgcacga cattcccggc gcgcggcacg
     2461 ggagcggcgg gccggccggc tacaccgcgc tggaccacct ggcgacgatg gtggccatcg
     2521 tccagcccga tggacgatgc gtgttcgtca atgcggcgtt cgaatccgtg ctcggcttgt
     2581 cgcgacgcag cgtgcttcgc ggctccctgt tcgactggtt cgtcgattcg cagccgctgc
     2641 gcgacaccgt ggcggcggtg gtccgcaacg aatacgcgac cagccggctg gaggcgcaat
     2701 tgcgccgcac ggcctcggcc cacgacgcgt tgccggtgca cgtgatcgtc aaccagatgg
     2761 atcgctccag caacgtggtg gtcgagctgg tcgaactgga gcagcagacg cggcaggacc
     2821 gcgaggagcg tgcgctcgac caggcgcagg ccaacaagga actgatccgc aacctggcac
     2881 acgagatcaa gaacccgttg ggcggcatcc gcggcgcggc gcagctgctg gagatggagg
     2941 tcgagtcgcg cgcgctgacc gaatacaccc aggtcatcat ccaggaggcc gaccgcctgc
     3001 aggcgctggt cgatcgcctg ctggcgccgc accgcaagcc gcacgtggtg agcgacgtga
     3061 acatccacga ggtgtgcgaa cgcgttcgct cgctcatcct ggccgaattt ccgcgcgggc
     3121 tgcaggtggc gcgcgactac gacatctcga ttcctgactt ccggggcgac cgcgagcagc
     3181 tcatccaggc ggtgctcaac atcgcccaca acgcggccca ggcgctggcc gagcgcgtgg
     3241 cgctgggcga cgcctgcatc acgctgcgca cgcgcatcgc ccgtcaggtg acgctgggca
     3301 aacagcgcta tcgtctggca ttggacttgc atatcgagga caacggcccg gggatctccg
     3361 agtcgattcg agatcgcatc ttctacccct tggtgtcggg acgcgatggc gggtcgggct
     3421 tgggtctgac gctggcgcaa accttcgtgc agcaacacca gggcatgatc gaatgcgaca
     3481 gcgttccggg cagaacgaac ttcaagatcg tgattccgct gccctgaacc gccggttcac
     3541 cgcagcgggg ccggaaccat gggcagggca tgaaacccat ctggatcgtt gacgacgacc
     3601 aatccatccg cttcgtgctg gagaaggcgc tggcgcgcga agagttcgcc gtgcgcagct
     3661 tcaccaaccc gcgcgacgtg ctggccgcgc tggacgagga ggcgccgcag gtgctggtgt
     3721 ccgacatccg catgcccggc ggctcgggca tcgagttgct gagcaaggtc aaggaacgcc
     3781 acccggggct gccggtcatc atcatgaccg cctactccga tctcgacagc gccgtcagcg
     3841 ccttccagg
//
LOCUS       DASWIY010000278         9261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_559361, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000278 DASWIY010000000
VERSION     DASWIY010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9261
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559361"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGA59_10180"
     CDS             <1..366
                     /locus_tag="VGA59_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9720087.1"
                     /translation="ALDNVGMPLRIRREPPEVCARQAAATLAAVGLAERLRHRPSELS
                     GGERQRVAIARALVTHPACVMADEPTGNLDRSTADVVFDLMLQLARERGTAFILVTHD
                     ETLAVRCGRVLRLSGGRLS"
     gene            complement(510..899)
                     /locus_tag="VGA59_10185"
     CDS             complement(510..899)
                     /locus_tag="VGA59_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9720088.1"
                     /translation="MPTLGLNHINLSGTRDVLDALKDFYCNVIGLEVGARPNFPRFGY
                     WLYAGSHPVVHLYESAADEARKTDVLTTFDHVAFTCEDCAAFEALLRRRGIEYSKAIV
                     PGSDQVQLFLRDPAGIKVELNFPAGGY"
     gene            1000..1596
                     /locus_tag="VGA59_10190"
     CDS             1000..1596
                     /locus_tag="VGA59_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX9720089.1"
                     /translation="MHQSLASILFPEYRRRVLGLLLLRPEEALHGREVARRTGLPAGT
                     ITRELTRLAAVGLLKREKRGNQQVYSADTGSQIYSELASILRKTSGMADVLVQALMPL
                     APSLRAAFIFGSVARGSETAGSDVDLMLIGDVTFKQAVELLYPVQTTLGREVNAQVLS
                     PSEFRKKARKQTFLVDVLAKPKIFLIGNEDDLEELAGH"
     gene            1568..2005
                     /locus_tag="VGA59_10195"
     CDS             1568..2005
                     /locus_tag="VGA59_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX9720090.1"
                     /translation="MTLKNLLGISLDQIKPDKAQIGKLLAAAERNIADAQLDGLSPES
                     RFDVAYKAIMQLAMVSLNSNGYRTLTSKPGHHQTAIQTLAQTVGVPQAEVIVLDALRK
                     QRNVSDYSGDLVSDAAVAECLASAKALLAHVRVWLRANRPELL"
     gene            complement(2102..3412)
                     /locus_tag="VGA59_10200"
     CDS             complement(2102..3412)
                     /locus_tag="VGA59_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9720091.1"
                     /translation="MLTDTKDQAVARALADAKSRYAAANPLSREQHEKAAKVLPGGNT
                     RTVLFTEPFPITLATGRGCRVTSLDGREYIDFLGDYTAGLYGHSDPVLKQALIGALDH
                     GWVMGGHIRLEEELARLICSRFASIELVRFTNSGTEANLFAISAARAISGRNKVMVFE
                     GAYHGGVFMFGGKPSSLNAPFDFLKAPYNDVDGTRALLEAQREEIGTIIVEPMLGSAG
                     CIPADKAFLGMLRDWATANGALLIFDEVMTSRLSPGGLQAIVGVAPDLTTLGKYVGGG
                     LSFGAFGGRADVMRRFDPLQPDAFSHAGTFNNNVFTMSAGVAGLRDVYTPSAALALNA
                     RGDALRQRLNTAAQGAKFPMQFTGWGSMMNVHMCPGAVRSVRDLEQSSRPLSDLFYFH
                     MLDRRVWIARRGMINLSRPIGDAECDHLVSAVEGFVETCNRLLA"
     gene            complement(3725..6919)
                     /locus_tag="VGA59_10205"
     CDS             complement(3725..6919)
                     /locus_tag="VGA59_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9720092.1"
                     /translation="MFKRILLTVLGKRPLSVHHTVVLLLAWLITPLAAGSAILVFALW
                     TDEHAKVEQELQGRSVGIAHSVELHLGHQRSQLEAIAALPEVDRGDLGAIYRFAQDVV
                     ADHPGTLVALVRPDGQLLVNTSVPFGTSLPNLWQLEDQRNEASWQGHALPVSSQGLTR
                     EVFRSGQIAYGGLFFGVTSRGPRLAMALPVKRGGAILYALMLSQPTDVLEGLLGNAGL
                     PREAGAVLLDRNGAVIATSGVFPYGVAQKLPLPGPGAALRELVAPDGTPMVAATAKTS
                     AGHTVLVGMVRADAYGTARTVAAGWGLVFVAALVLSILGAARATRRLADPLGRLAAGA
                     LAGGPLLTESSNIHEIDLLNAALEAGARNAELRREDQLRRIEAEKEEEAVKASEAQMR
                     RAFDSIYASVAVLDLDGRVIEVNRAALRRVDVSRAEVVGQLFWDCYWWSHDPLVQHTM
                     RESIAEAREGETVRYNIPARVENGRLVMVDFQLSAMRDEAGEVTQLIACAVDVQDRVD
                     AIRSLQAREAQAHEVAHKLDEQRWLLDAALQVTPAGILVSDGSGRMLRMNQANQRLWG
                     PAPLSASIDEYAAWKGWWAQGQEREGQAVQPHEWPLARALKSAQPESSVIDIEPFDAP
                     GVRKTVEIRAEPVLDEKGVVVAGVVVQMDITDRVRAEAALRQADRHKDEFLATLGHEL
                     RNPLGPIRSAIYILRSHPTAEPALIRAQEVIERQTKHMTRLVDDLLDVARITQGSIRM
                     EQESVALQDVVAAAAEAVAPALDAKRVRLRQELAEEPLFARGDATRLSQALVNLLTNA
                     CKFTHPEGQVVLRLRRDDAAAIIEVEDSGIGLASDSLERIFGLFVQEEPSGAAGNSGL
                     GIGLALSRNLLVMHGGALSAASAGLGKGSTFTARLPLAAEPAPVPVPVIEMKQERPSR
                     RHRLLVVDDNRDGCDTMKELLEISGFDVDTAYDGATALIAVQKTSHDALVLDIGLPDI
                     NGYELCRRIRAHLGHAPVMIALTGWGQAEDIKAAERAGFDAHITKPADPDVLCNTLAK
                     LLKERERDGDEAAGGEGHRAQGVPGAAASPSVAG"
     gene            complement(7252..7647)
                     /locus_tag="VGA59_10210"
     CDS             complement(7252..7647)
                     /locus_tag="VGA59_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9720093.1"
                     /translation="MTILVAYVPRPEGDAALAKGIEIAKRRNEPLMVVNASPGGPKED
                     ASMADTLDVERIEKQLSAVPGLNAQFKQFVRGKNAVEEIEALVESLPASLLIIGIRKR
                     SAVGKLILGSVAQELLLSVSCPVLAVKAG"
     gene            7840..8736
                     /locus_tag="VGA59_10215"
     CDS             7840..8736
                     /locus_tag="VGA59_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX9720094.1"
                     /translation="MNKILVLGGTGFVGRHLCKELARLEYSVTVPTRNISKAQAVQSV
                     PLLDLVRADVHDEPTLERLVAGHDAVVNLVAILHGSSFEFQRVHVDLVKKLTRACARA
                     GPLRLVHVSALGAATDAPSMYQRSKAGGEAVIQACGLDWTVLRPSVMFGEGDRFLTVF
                     ARLQAIFPLVPLAGADTLFQPVWVGDVVSAIVQCLQRNDTGKRTYEACGPEVFSLKQL
                     VELAGSLSGHPRLVVGLPPVLGRLQALLMELAPGPRMMSRDNLDSMKVNNVASGKLPG
                     LDALGISPSSLGAIAPSYLRNQ"
     gene            complement(9087..>9261)
                     /locus_tag="VGA59_10220"
     CDS             complement(9087..>9261)
                     /locus_tag="VGA59_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720095.1"
                     /translation="YFYAGTATDLKKVYETLSSRLTVEKKETEISALFAMAAAALALL
                     SAGLSLLWFNRIL"
BASE COUNT         1704 a         2968 c         2892 g         1697 t
ORIGIN      
        1 gcgctggaca acgtgggcat gccgctgcgc atccggcgcg agccgccgga ggtatgcgca
       61 cgtcaagccg ccgcgacgct ggcggcagtg ggcttggccg agcgtctgag gcaccgcccg
      121 tcggaactct ccggcggcga gcgccagcgc gtggccatcg cccgcgcgct tgtcacgcac
      181 ccggcgtgcg tcatggccga cgagcccacc ggcaacctgg accgcagcac ggcggatgtg
      241 gtgttcgacc tgatgctgca actggcgcgc gagcgcggca cggctttcat cctggtcacg
      301 catgacgaga cgctggcggt gcggtgtggg agggtgttga ggttgagtgg gggacggctg
      361 agctagaagg gtgcccggca aagagtcttg tcattgcggg ctcgacccgc aatccatgca
      421 gtccaaagct gtcatgccgg accccggatc aagtcggggc aggccagatc gggcatccat
      481 ctcggtgagc cgtttacctc aacctggcat cagtaaccac ccgccggaaa attgagctcc
      541 actttgatgc cggccgggtc cctgagaaac aactgcacct gatcgctccc cggcacgatt
      601 gccttcgagt actcgatgcc gcgccggcga agcagcgcct cgaaagcggc gcagtcctcg
      661 caggtaaacg cgacgtgatc gaaggtcgtc aaaacatccg tcttgcgcgc ttcatccgct
      721 gcggattcgt acagatgaac gacgggatgg cttcccgcgt aaagccagta cccaaaccgc
      781 ggaaaattcg gccgcgctcc aacttcaagg ccgatgacgt tgcaatagaa atccttcaac
      841 gcatcgagca cgtctcgagt tccgctgagg ttgatgtggt tcaggccaag agtgggcatt
      901 ccggctcctt gcgaacgaaa gggcaagtgc cagcatacat cgccactact agcatcacat
      961 tgccaagtga tgcaaataaa gcgacaatcg acgccttaaa tgcatcaatc cctcgcctcc
     1021 atcctgtttc ccgagtaccg acgccgggtg ctcgggctgc tgttgctgcg ccccgaagag
     1081 gcgctgcatg gccgcgaggt tgcccggcgt accgggctgc ctgccgggac catcacgcgc
     1141 gaattgacca ggcttgcggc cgtgggccta ctcaagcgcg agaagcgcgg caaccagcag
     1201 gtgtacagcg ccgacactgg gtcgcaaatc tattccgagc tggcgagcat tctgcgcaag
     1261 acatccggca tggccgacgt gttggtgcaa gcgcttatgc cattggcccc atcactgcgc
     1321 gccgcgttca tattcggatc cgtggcccgt ggcagcgaga cagcgggcag cgacgtcgac
     1381 ctgatgctga tcggcgatgt cacgttcaag caggcggtcg aactgctcta tccggttcaa
     1441 accacgctgg gccgtgaagt gaatgcgcag gtgttgtccc cgtccgaatt caggaagaag
     1501 gcgcgcaagc agaccttcct cgttgacgtg ttggccaagc cgaaaatctt cctgataggg
     1561 aacgaagatg accttgaaga acttgctggg cattagcctt gaccagatca agcccgacaa
     1621 ggcgcagatc ggcaagctgc tcgcggccgc cgagcgcaac atcgccgacg cacaactcga
     1681 cggcctgagc cccgaatccc gattcgacgt agcgtacaag gccatcatgc agctggccat
     1741 ggtgtccctc aactcgaacg gctaccgcac attgaccagc aagccgggcc accaccagac
     1801 cgccatccag acgttggcgc aaacggtggg cgtgccacaa gccgaagtga tcgtattgga
     1861 cgcgttgcgc aagcagcgca acgtctcgga ttactccggc gacctcgttt ctgacgcggc
     1921 ggttgcagag tgcctggcca gtgcgaaagc acttctcgcg cacgtgcggg tgtggctccg
     1981 agcgaacaga ccggagctgc tttgattcgc tcgaattgat gctcggaacc aggaattagc
     2041 aaagggttgt tcttcctcgt cattgcgggt gggccggtcc ggccgacagc cgctctcgct
     2101 cctacgcgag caacctgttg cacgtctcga caaagccttc aaccgcgctc accaggtggt
     2161 cgcactccgc atctccgatg ggccgcgaca gattgatcat cccgcgccgc gcgatccaga
     2221 cacgccggtc cagcatgtga aagtagaaca ggtcggaaag cggcctcgaa ctttgctcca
     2281 gatcgcggac gctgcgcacg gcgccagggc acatgtgcac attcatcatc gatccccaac
     2341 cagtgaactg catcgggaac ttggcccctt gcgccgcggt gttcaggcgc tgacgcaacg
     2401 cgtcgccgcg cgcattcagc gccagcgcgg ctgaaggcgt gtacacgtct cgcaggcccg
     2461 ccacgcctgc cgacatggtg aatacgttgt tgttgaaggt gcccgcatgg gaaaacgcgt
     2521 ccggctgaag cgggtcgaag cgacgcatca cgtccgcgcg gcctccgaac gcgccgaaac
     2581 tcaagcctcc tccgacgtac ttacccagcg tcgtgaggtc gggcgcaaca ccgacgatcg
     2641 cctgcagccc gccgggcgac aggcgcgagg tcatcacctc gtcgaagatc agcagcgcac
     2701 cgttggcggt ggcccagtcg cgcagcatcc cgaggaaggc tttgtcagcc ggtatgcaac
     2761 cggccgagcc gagcatgggc tcgacaatga tggtgccgat ctcttcgcgt tgagcctcga
     2821 gcagcgcccg ggtgccgtcc acatcgttgt acggcgcctt caagaaatcg aacggcgcgt
     2881 tcaaggagct cggcttgccg ccgaacatga acacgccgcc gtggtaagcg ccctcgaaca
     2941 ccatcacctt gttcctgccg ctgatggcgc gcgccgcgga gatggcgaag agattcgcct
     3001 cggtgccgga attggtgaac cgaacgagct cgatcgatgc gaaacgggaa cagatcaggc
     3061 gggcaagttc ttcttccagg cggatatggc ctcccatcac ccagccgtgg tctagcgctc
     3121 cgatcaaagc ctgcttgaga acaggatcgg agtggccgta caacccggcc gtgtaatcgc
     3181 cgaggaaatc gatgtactcc cgtccatcca gcgaagtcac cctgcaaccc cggcccgtgg
     3241 ccagcgtaat cggaaacggc tcggtgaaaa ggacggtgcg ggtgttgccg ccgggcagca
     3301 cctttgcggc cttctcgtgc tgctcacggc tgagcggatt ggcggcggca tatctcgatt
     3361 tcgcgtccgc gagcgcccgg gcgacggctt gatctttcgt atcggtcaac atgggagcac
     3421 ctccatcttc ggcgtttgcg ggatggctca cgccgccttc cgatcgagcc agtcgcgagt
     3481 gcggtcagct ggcgcaggcc tacgcggcgt ggcaggccaa tttcgaagcg gcgccgtttt
     3541 tccgctcgat tgagccgcac gaccaggatc acctggaacg attcggatat cgattggcac
     3601 ctttcaacaa gagcgacaac cgtttcggtg gatctgcagg cggaatgaca agaggatgca
     3661 tggattgcgg gtcaagcccg caatgacaat tcaaagaacc ggcaggagca gctgcgacgg
     3721 ccgctcaccc ggccaccgaa ggcgacgctg ccgccccagg aaccccctgc gctcgatgac
     3781 cctcgccgcc agccgcctca tcgccatccc gctcccgctc tttcaacaac ttcgcaaggg
     3841 tattgcaaag cacatccgga tccgccggct tggtgatatg agcgtcaaac ccggcgcgct
     3901 ccgcagcctt tatgtcctcc gcctggcccc acccggtcag cgcgatcatc acgggcgcgt
     3961 ggccgaggtg ggcacggatg cgccggcata gctcgtaccc gttgatgtcg ggaagtccaa
     4021 tgtccagaac cagggcgtcg tggctcgttt tctgtacagc gatgagcgcg gtggcgccgt
     4081 cgtaggcggt gtcaacgtca aagccgctga tctccagcag ttccttcatc gtgtcgcagc
     4141 catcgcggtt gtcatccacg accaacaggc ggtgacgccg gctcgggcgc tcctgtttca
     4201 tttcgatgac aggcacgggc accggtgccg gctcggccgc cagcggcaga cgggcggtaa
     4261 aggtgcttcc tttgccgagc ccggcgcttg ccgccgagag tgccccgccg tgcatgacca
     4321 ggaggtttct ggacaacgcc aggccaatgc ccaggccgct gtttcccgcc gcaccgctgg
     4381 gttcctcctg aacgaacagg ccgaagattc gctccaggct gtccgatgcc agcccaatgc
     4441 cgctgtcctc cacctcgatg atggcggccg catcgtccct gcgcaggcgc aggacgacct
     4501 ggccctcggg atgcgtgaac ttgcaagcgt tcgtcaacag gttgaccagc gcttgcgaaa
     4561 gccgggtcgc gtcgccccgc gcgaacaagg gctcttccgc caattcctga cggagccgca
     4621 ctcgcttcgc gtccagcgcg ggcgcgacag cttccgctgc cgcggcgacg acgtcctgga
     4681 gagccacgct ctcctgctcc attctgatcg agccctgcgt gattcgcgcc acgtcgagca
     4741 ggtcgtccac caggcgggtc atgtgcttcg tctggcgttc gatcacttcc tgcgcccgga
     4801 tcagggccgg ctcggcagtg gggtggcttc tgaggatgta aatggccgat cgaatgggac
     4861 ccagcgggtt gcgcagctcg tgccccagtg tggcgaggaa ttcgtccttg tggcggtcgg
     4921 cttgtcgaag cgcagcctcg gcccgcacgc ggtcggtgat gtccatctgg acgaccactc
     4981 cggccacgac gacgcctttt tcatcgagca cgggttcggc tctgatttca acggtcttgc
     5041 gcaccccggg ggcatcgaac ggttcgatgt cgatcaccga ggactcgggc tgtgcgctct
     5101 tgagtgcgcg ggccagcggc cactcgtgcg gctggacggc ttgaccctcg cgctcctggc
     5161 cttgcgccca ccaacccttc caggcggcgt attcgtcgat ggaggccgac aacggggctg
     5221 gtccccacag gcgctggttg gcctggttca tgcggagcat ccggccgctt ccgtccgata
     5281 cgagaatgcc cgccggtgtc acctgcagcg ccgcatcgag cagccagcgc tgctcgtcga
     5341 gcttgtgagc gacttcgtgc gcctgcgcct cccgcgcctg cagggacctg atggcgtcca
     5401 cccggtcctg gacgtccacg gcacaggcga tcagctgcgt gacctcaccg gcctcatccc
     5461 tcatcgcgga cagctggaag tcgaccatca cgagccggcc attctccacg cgcgcaggga
     5521 tgttgtagcg caccgtctca ccctcgcgcg cctcggcgat cgattcgcgc attgtgtgct
     5581 gcaccaaggg atcgtggctc caccagtagc agtcccaaaa cagttgcccg accacttcgg
     5641 cccggctgac gtcaacccgc cgcaacgcgg cacgatttac ttcgatcacc cgtccgtcga
     5701 gatcgagcac ggcgacggat gcgtagatgc tgtcgaaggc acggcgcatt tgcgcctcgc
     5761 tggccttgac tgcttcctct tccttctcgg cctcgatgcg ccgaagttga tcctcgcgtc
     5821 gtagctctgc gttccgggcg ccggcctcaa gcgctgcatt cagcaagtcg atttcatgga
     5881 tgttgcttga ttcagtgagc agtggcccac ccgccaaagc gcctgcggcc agccgcccca
     5941 gcggatcggc aaggcgacgg gtggcccgtg cggcgccaag gatgctcaat accagcgccg
     6001 ccacaaagac gagtccccag ccggcagcga ctgtgcgcgc cgtgccatag gcgtccgcac
     6061 gcaccatgcc caccagcacc gtgtgtccgg cgcttgtctt ggcggtggcg gcgaccatgg
     6121 gtgtaccgtc cggcgccacc aattcccgca gcgcggcgcc cggccccgga agcggaagtt
     6181 tctgtgcaac cccatacggg aaaacgccgc tggtcgcgat gaccgcacca ttgcgatcga
     6241 gcagcaccgc accggcctcg cgcggaaggc cggcgttgcc cagcagccct tccagaacgt
     6301 ccgtgggctg cgacagcatc agcgcgtaca agatcgctcc gccgcgcttg acgggaaggg
     6361 ccatggccaa tctcgggccc cgggaggtca caccaaaaaa caagccgccg taggcgatct
     6421 ggccgcttct gaaaacctcg cgtgtcaggc cctgcgaact gacgggaagc gcgtgtccct
     6481 gccaactggc ttcatttcgc tgatcttcca gctgccagag gttggggagc gatgtcccga
     6541 aagggacgga ggtattgacc aggagttgcc cgtcgggccg gaccagcgcg acgagcgttc
     6601 ccgggtgatc cgctaccacg tcctgggcaa agcggtatat cgcgcccagg tcgccacgat
     6661 ccacttcagg aagggccgcg atcgcctcca gctgcgaacg ttgatggcca aggtgcagtt
     6721 cgacggaatg tgcgatgccg acggaccgtc cttgaagttc ttgttcgacc ttggcgtgct
     6781 catcggtcca cagtgcaaac acgagaatgg cggagccggc ggcgagtggg gtgatcagcc
     6841 acgcgagcag gagcacgacc gtgtggtgca cactcaacgg cctctttcca agcaccgtca
     6901 gaagaatccg cttgaacact cctgccttcc cgccgggcgc cgggcgcctt tgggcgcgcc
     6961 catggaagcc aactcccctg ttaccgattt gaaagtactc tttgcttgtg tgagctggtt
     7021 tgacaaagct caacagcgcc ctggatagcg aggctgctgc cgcttgaggc gaagtcaatc
     7081 aaaaaacata gcgatcaagg ttcgtgtaat caaaaatacc gttacataag gggagagcca
     7141 tggcgggact ttgtccgctc accagcgcaa gcaggtcaag ggcgaggttc ccgccgacta
     7201 tttcgacgcg atcgagcgaa cggtcgtcga agcgttcggg cttggacgtt tctagccggc
     7261 tttgacggcc agcacaggac acgaaacgga aagcagaagt tcctgggcaa cactgcccag
     7321 gatgagtttg cccaccgccg agcgcttcct gatgccgatg atgagcaggg atgccggcag
     7381 ggactcgacc agggcttcga tttcctcgac ggcgttcttg ccgcgaacga actgcttgaa
     7441 ttgcgcgttc aggccgggca ccgccgacag ctgtttttct atccgctcga cgtccagggt
     7501 atcggccatc gatgcatctt ccttcggccc gccgggactg gcgttgacca ccatcagggg
     7561 ttcattgcgg cgtttggcga tttcgatccc cttggccagg gcggcatcgc cttcgggccg
     7621 cgggacataa gctacgagta tggtcatctt tcgctcctat tgcgctgcag ttgctgcaga
     7681 tcttgataaa cgtgggcact tctccgccga caacaaacag cgtggggccg gaccccttcg
     7741 tgaatactac gcccctttgc ggcgaacaag ttccgggcga gttatgctgg tgatgcttgc
     7801 gtttcaaaca ccggcttgcg ggccggggag ggagctgcaa tgaacaagat tcttgtcttg
     7861 ggcggtacag gatttgtcgg ccggcatctg tgcaaagagc tggctcgcct ggaatacagc
     7921 gtcaccgtcc ccacgcgcaa tatttccaag gcgcaggctg tgcaatcggt gcccctgctg
     7981 gacctggtgc gggccgacgt gcatgacgag cccacgctcg aacgtttggt ggcgggccac
     8041 gatgccgtcg tcaacctggt cgccatcctg cacggcagct cattcgagtt ccagcgtgtt
     8101 cacgtggatc tggtgaaaaa gttgactcgc gcctgcgctc gtgcggggcc gctgcgcctc
     8161 gtgcacgtga gtgcgctggg cgccgcaacc gacgctccat cgatgtacca gcgcagcaag
     8221 gccggcggcg aagcggtgat ccaggcctgc ggtctggatt ggactgtgct gcgccccagc
     8281 gtcatgtttg gcgagggaga tcgattcctg acggttttcg cgcgcctgca ggcaatcttt
     8341 ccgttggtgc cgctggcggg cgcggatacg ttgttccagc cggtatgggt gggcgatgtc
     8401 gtcagtgcca tcgtgcaatg cctgcaaagg aatgacaccg gcaaacgaac ctacgaagct
     8461 tgcgggccgg aagtgttttc attgaagcag ttggtcgagc tggcgggcag tttgtccggg
     8521 caccctcggc tggtggtggg tttgccgcct gtgctgggcc gattgcaggc gctgctgatg
     8581 gaactggctc ccgggccgcg catgatgagc cgggataatc tggattccat gaaggtgaac
     8641 aatgtggcga gcggcaagct gcccggcctg gatgcgctgg ggatctcacc cagttccctg
     8701 ggtgccatcg ccccgtcgta cctcaggaac cagtagcagc gaagcagcgc ccggaactag
     8761 tgaatcacgc gcctggtgcg aagcgctcat cgtgtcgcgc tagttcctgg atccctcgtt
     8821 tctcgtacaa ctcagacgcc gaaacggcta ggtccggcgg tggccgattc tgacttctcg
     8881 gtcccgcgac gatccgcagg ggcaatgagc gctgccgcgg cgtgctccag ccgaatgaga
     8941 ggatgcatgg attgcggctc aaggccgcaa tgacaaacca ccatggattg cggctcaagg
     9001 ccgcaatgac aagaatggct gtcatgccgg gcttgacccg gcatccatga cttcctggat
     9061 tgcggctcaa ggccgcaatg acaagatcac aggatccggt tgaaccaaag cagcgacagc
     9121 cccgccgaca gcaaggccag cgccgccgcg gccatggcga acagcgccga gatttccgtt
     9181 tccttctttt ccaccgtcag gcgtgagctc agcgtttcat agaccttctt gaggtcggtc
     9241 gccgtgccgg cgtagaagta a
//
LOCUS       DASWIY010000279        16472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2043481, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000279 DASWIY010000000
VERSION     DASWIY010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 16472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16472
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043481"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VGA59_10225"
     CDS             <1..815
                     /locus_tag="VGA59_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720096.1"
                     /translation="FLSPADKRLLSQVQGRTYAYVFALVERFISAKVLDISREHWLGD
                     QVALEALVRMSDEEIKHQEMFRRLEAQMTQDMPDGYVQTADPNAVARAVLGKSTWAVL
                     ALTLDIELFVQAHYRASIEPQDDLSELWKDVFLFHWKEESQHAILDEIELINEDRKLS
                     VAQRSDAVDDLIALVGAVDGILQAQAMADGDYFTQAAGSHFTDAQREQIHEKVLKAYR
                     WQYIVSGVMEPRFQKVLFGLLDENQAVRIKNALAPLTYAIPDQPEQPLPLAA"
     gene            880..1164
                     /locus_tag="VGA59_10230"
     CDS             880..1164
                     /locus_tag="VGA59_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720097.1"
                     /translation="MSYAVQSRRAATAQPRSVRFADGLHSALRTFMRELAGAWRYAVA
                     RREFNRLDAGALRDLGMSQSEFDSYWAESHGLAEPTRVRVIEGVRRRSGP"
     gene            1161..2399
                     /locus_tag="VGA59_10235"
     CDS             1161..2399
                     /locus_tag="VGA59_10235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9720098.1"
                     /translation="MNAAGWRLTLAAAVLMGLVVGGRSAFGLFVSPLNTASGMGLAAL
                     SFALALGQLGIGFAQPVIGALADRHGAARVIVAGALVLAATLAVPSVWPVPMAVSLAL
                     VSSAVAGSAVGSNGLLLGEVARAVSPARAGLAVGLVGAGASAGQLVLGPATQWAIDEQ
                     GWAVALLATAALSLIALPLAIPFRRQSPVKAAQPSQPVGDVLREWRFWRVAASFGVCG
                     FHIAFLAVHMPGVIERCGLPPSLAGTWIALAGAANIAGSIAVGFALKRHDAGVLLGSL
                     YATRALGIAALLVLPVTPMLMLGFAVVMGASHMATLPPTAQLVARQHGVQRLGTLFGI
                     VMLVHQVGSFAGIWLGGWAVEATGSDNLLWCVDAGLALCAAALVWPRPRAAESASVPR
                     LRASPVPARATALAQRRRIP"
     gene            complement(2444..3946)
                     /locus_tag="VGA59_10240"
     CDS             complement(2444..3946)
                     /locus_tag="VGA59_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9720099.1"
                     /translation="MRHEHILQTVGRTPVVRLSRLAPAGIELFVKLEAFNPMGSVKDR
                     MALAIIEDAEASGRLVPGQTVVEATSGNTGIGLAMVCARKGYPLVIVMAENFSVERRR
                     LMRFLGASVVLTPAGLKGSGMLAKARELAQAHGWFLPSQFDNEANAAAHERTTALEIV
                     QDFHDRPLTHWITGAGTGGTLLGVGRALRRHSPQTRIVVCEPDNAPVLGSGIAAPPPA
                     TDGTPSSHPHFRPHLMQGWTTDFVPALVQRAREEGLIDRVQAVPGARALETARELARK
                     EGILAGISGGATVAGALLLASELPPGSRLLAMLPDTGERYLSTPLFSDIDSEMNAEEW
                     AISRSTPRARFDALSPAAPTPAPAPAPLPAVTPWGRDFVERAVIEPARPVVMFALEWC
                     EFCWTIRRLFKRIGVPLHSIDLDAAAWHAEGRGLQIRAALHAHTGQPTIPQVFVGGQW
                     LGGCMDTLAAWREGRLQALLIRNSVPFQAEAIGDPSELLPAWLQPAAPVR"
     gene            complement(4077..8195)
                     /locus_tag="VGA59_10245"
     CDS             complement(4077..8195)
                     /locus_tag="VGA59_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9720100.1"
                     /translation="MSSQASPTGPTEAEAIPLTQVQALRRVALAVAHPGGPNLFADLA
                     RELAASLQVGLVFIAVFADDSRTVMRTLAACLDGRLLRNFDYRLEGSPCAAVVGRSCQ
                     FIESGLLPRLAPESMSARKRMDSFAAYPLNDSAGEPLGLVVAMDRRPIAAGDADHAEA
                     MLKIIAGRVAAEIERERTDEALRAVALAVSGSRSGTVFDELVRLLATILHVEVAFIAR
                     YEPARPNSLRMLAMYYDGQVVQDITYPLAGTPCETVLGQRFRAYPADLQSLFPDDQDA
                     REQCTVSYAGHPLVALDGSPLGIVSVASRRPIPPAQVDRVEAMLKIFAVRAAAEVERL
                     RASEALQRSEASYRAIFEASEDAVFIHDWETGAVLDVNRKACDIYGYTFEELRRSTMA
                     DTSSGVFPYTAEEALRLMQSAKLGRCPPFEWQRRNKDGSLHWDEVRLKPAMIDGRPHI
                     VAFSREITAQKQALEDLRLREEQYRAIFESSLDGLFMWDEDLRIVDVNPAGLALYGYR
                     REDVIGRTFPKTMPQAYVRERLQMVRRAMAGETTHVETTVLRPDGTTFDADLRVLPFV
                     QRGQAHALAVVRDISERRRRERELQRSEARLRATVEAAFDAVVGMDSDGLIVEFNAAA
                     ERVFGHRREDVFGRPLAEMMLPKRHHDAYEREMRNLRASGGGPMAGRLVETKMLRADG
                     SEIPVELAMSVAVVPEGNIFVGHLRDITERQRADQALRDSEEQYRAIFNASADALVLR
                     AADFSIVDVNATYEAMSGYSRAEVLGVNRVLANPPEVVATIRALHEKALAGEAVSLET
                     QMFRRDGRRYDLELRGVAIQHRGEPHVLYMGRDITQAKSAERALRDSEEQYRAIFNAS
                     ADALVLRDADYRAVEVNPAYTTMSGYTREEVMAANHVLTQADGEVRARHRSDHELALA
                     GQELRFEVTATRKDGSTMQAEVRGTPMMYRGRPHVLYASRDVTERNAAEQRRRELERQ
                     LRQAQKMEAIGQLTGGLAHDFNNILTSVLGYVTMAQERPTAGEDPVLARQLGQARLAA
                     ERARDHVAQLLAFSRPRRGERRLLAPAPVARQALQLLRPNMPSSIAVECPGICSDTDP
                     AVPPVVADPVQLEQVLFNLCINARDAIRDHGTIRVRIGYSETAGHCASCSAPLDDSRW
                     VWVEVADDGRGMPREVMDRMFEPFFTTKEVGRGTGMGLAMVHGIVHDHGGHIQVVSTP
                     GQGSLFRVLLPAAAEDTRRTDPPAAPAPVVRAAPSLRGRVLLVEDESIVSDFMMDVMT
                     GWGLDVALERDPLAAARRLASEEEVFDLLLTDQTMPGLTGLALSKHAVRHRPALPVLL
                     YTGNASEIGQAELAECGVSALLRKPIDSDALRSLLRELLERPGTEKVPAAFA"
     gene            8399..9220
                     /locus_tag="VGA59_10250"
     CDS             8399..9220
                     /locus_tag="VGA59_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9720101.1"
                     /translation="MSIIRHLFGNRSRSTPEVVIALHCSGSSGRQWDAYAKLLPRGMR
                     LAAPELMGYCPRECWECGTPVSLEAEARRLGPLLFSHTGGVHLVGHSYGGAVALQMAL
                     RWPDRVKTLTLFEPVRFALLLEDHDQEATWKAIVSIGRRIGWHVLSGRLDEAAALFVD
                     YWSGSGAWERLSAARRRAIAERMPKVRAEFEALFADTAPAWWFRKLEMPVRLITGSTS
                     PLPARRVADIIARHCAQAEVVRLEGVGHMGPISHAPLVASHLAFQARDAQLALAA"
     gene            9401..9940
                     /locus_tag="VGA59_10255"
     CDS             9401..9940
                     /locus_tag="VGA59_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX9720102.1"
                     /translation="MDIMIRWFPSRESLMRSRWLGPLAHHFQDESLWQVERGSIARGV
                     AIGLFFGLLLPLAQFLFAVAFAILLRGHVAVAAAATLVSNPLTFAPLYWLAHRIGSAL
                     LGSSRAEARVHAAMVEAQVEVTAAAQGWLRAIWEAVLNAGAPLLLGLAVMAVAGAVLG
                     FLVVWLLWRPRRDGRPNAG"
     gene            10043..11506
                     /locus_tag="VGA59_10260"
     CDS             10043..11506
                     /locus_tag="VGA59_10260"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720103.1"
                     /translation="MAPFLSRLARDGCAGKAFVPALNLLVAVLLSLTGTLARAALPVL
                     APSPFADGTQVDTAVLGYRIPVILVHGLGGSTEGWDRFLRAYEQSPGWRDAFKPYSFR
                     FGSTTAEVIFDPAAPRTISALGAALRDAMQGFYDRPEAAPDFGFGNKRVIVLAHSMGG
                     LVARSMMQEHVFSDGQRGGQKVLHLITLGTPHHGTPLADAAIALGLHTVSELDEGYFA
                     FVADTAWTNFDGLNMSGGRCNHWLAQLNNYAPATGGNYGRCGAVPANPLPGYYWKIIA
                     YGASALQNRDWYLGETGVYKPGSSTSLLFPYNYLRGALPRSYPNDGIVPLVSAQLEGA
                     SLWLRRQAYDCDHRYIRRGYPEFVRSPASTYTDWAFCAGTDSGQSYASGISGGYAVSG
                     SIMGVAGGIVDTIKTASEMERVLNWVEWKFPGYLAPAGATSDIVGKHYYRYYSSTEAY
                     LAVSSGNLYYLGAASGSPLVFLGTLGDLLPHAQAAGF"
     gene            11514..12362
                     /locus_tag="VGA59_10265"
     CDS             11514..12362
                     /locus_tag="VGA59_10265"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9720104.1"
                     /translation="MHLLRRHLIGLLAGLAALVCAHAPAQTDEEVPFITSPDDVTLEM
                     LRIAGVAPGDHVIDLGSGDGRIVILAARRFGATGLGVEIDPELVRKSMRHAREAGVAQ
                     RVTFREQDLFQTDLSAASVITMYLLPQVNLQLRPALLALKPGTRIVSRDWDMGDWRPD
                     RTTVVPVQDKKVGLDKSSKVHLWVVPARVEGLWCGTGLLREFSLQLSQLYQQVNGSLA
                     RRDRARAIEGRIDGATVRTQATRHGSLVLALVGDELSITGGEGTLALARGAAFRRAAD
                     RGSCDG"
     gene            complement(12377..12793)
                     /locus_tag="VGA59_10270"
     CDS             complement(12377..12793)
                     /locus_tag="VGA59_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9720105.1"
                     /translation="MPVLDSYLFFNGNCAQAMRFYETALGGKLEMMMTYGQSPEPEHC
                     PAGSEDRIMHASLKIDGRSLMASDIPANQPSAGMNGFSLSLNYASADEARRVFDTLAD
                     GGKVIMPLGKTFWAEAFGMLTDRFGTPWMVGGGQSS"
     gene            13116..13766
                     /locus_tag="VGA59_10275"
     CDS             13116..13766
                     /locus_tag="VGA59_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720106.1"
                     /translation="MSAGPFAEMLLLAAALAASLVVRPWRMLRGADSGSGLATPLLAA
                     LTLLPWLWAWPSSAAMPIPIQWSGAALAVLMLGWPLAVPVLVAAGISTVFTTGASWPV
                     ALSTIVWSGLLPATIVLLLGYAMRRVFGTHPAVYLLGRAFAVPLLALFGCALAGAMTS
                     GTLAGEDREMQVVAAFLMAMAETTWTCAVASILVAWRPQWLATWSDALYLGPRRRA"
     gene            13849..15276
                     /locus_tag="VGA59_10280"
     CDS             13849..15276
                     /locus_tag="VGA59_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX9720107.1"
                     /translation="MSASPRITRRSLLLAGVPASLLQAGCAAGRSAITPRQVELAAAP
                     AQVFLRGSAQPPVAAWSYGGSVPGPEIRVRQGERLRVVVHNRLAQGTTVHWHGVRVPN
                     AMDGVPHLTQPPIEPGARFVYEFDVPDAGTYWYHSHLHSAEQLERGLYGAFIVQEPQP
                     LEVDRDLTWVLDDWRLTREGRLNESFNDPHDVAHAGRLGNVVTVNGSFPRDLALRRGE
                     RVRLRLINAANARIFSLQFDGHRPRVVAIDGQPVMPHEPAGGRVTLAPAMRYDLVLDA
                     IGEPGKSYAVGDVFNPRAAYDLLQLAYGPTVLREQFLEAPVALAPNALPEPDIDSALR
                     HEIRFEGGMMGRMHQALLNGKPLNMMGLLRQGKAWAVNGVVADGHDMKPMLSLQRGRS
                     YVLALHNDTRWHHPMHLHGHSFRVIRRNGQPTRYREWQDTVLMDPQERVDIVFVADNP
                     GDWMFHCHILEHQDGGMMSVVRVSA"
     gene            15452..>16472
                     /locus_tag="VGA59_10285"
     CDS             15452..>16472
                     /locus_tag="VGA59_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX9720108.1"
                     /translation="MPEKVPATLIPGDGIGPEIVDAALAALDALGSPFEWDRQTAGMA
                     GVKDSGDPLPAAALASIRRTRLALKGPLETPSGGGFRSSNVRLREEFQLYANLRPART
                     IIPGGRFDKIDLVVVRENLEGLYIGHEHFVRVDDDPHAVAMATGINTRQGSRRLLEFA
                     FDHALATGRRKVTIVHKANILKALTGMFLETGVALYEQNYKGRFELDTVIVDACAMKL
                     VLNPWQFDMLVTTNLFGDVLSDLVAGLVGGLGMAPGGNIGCDAAIFEAVHGSAPDIAG
                     KGIANPVALLLAAAMMLDHCASPELAARLRKAIGETLNIDQVRTGDLGGHANTAAFTK
                     ALVSRI"
BASE COUNT         2530 a         5557 c         5585 g         2800 t
ORIGIN      
        1 cgttcctgtc cccggccgac aagcgcctgc tcagccaggt gcagggccgc acttatgcgt
       61 acgtgttcgc cctggtcgag cggttcatca gcgccaaggt gctggacatc agccgggagc
      121 actggctggg ggaccaggtc gcgctggagg ccctggtgcg catgagcgac gaggaaatca
      181 agcaccagga gatgttccgg cgcctggagg cgcagatgac tcaggacatg cctgacggct
      241 acgtgcagac cgccgacccg aacgccgtgg cccgcgccgt gctgggcaag agcacctggg
      301 ccgtactggc cctgacgctg gacatcgagc tgttcgtgca ggcccattac cgggccagca
      361 tcgagcccca ggatgacctg tccgagctgt ggaaggacgt gttcctgttc cactggaagg
      421 aagaatctca gcacgcgatc ctggacgaga tcgagctcat caatgaggac cgcaagctga
      481 gcgtcgcgca gcgctccgat gccgtggacg acctgatcgc gctggtgggc gcggtggacg
      541 gcatcctcca ggcgcaagcc atggccgacg gcgactactt cacgcaagcc gccggatcgc
      601 atttcaccga tgcccagcgc gagcagatcc acgaaaaggt gctgaaagcc taccgctggc
      661 aatacatcgt gtcgggcgtc atggaaccgc ggttccagaa agtgctgttc ggcctgctcg
      721 acgagaacca ggcggtgcgc atcaagaacg cgctcgcgcc gctcacctac gccattccgg
      781 atcaacccga gcagccattg ccgctcgcag cttgagcccg cccggaccgc gctgtgcggc
      841 ccgagcgcgg cccggcgttc actttcaaag gagattccca tgtcctatgc tgtccaatca
      901 cggcgcgccg ccacggcgca gccccgttcc gttcgttttg cagacggcct gcacagcgcg
      961 ctgcggacgt tcatgcgcga gcttgcaggc gcctggcgct acgccgtggc ccgccgcgag
     1021 ttcaatcgtc tcgacgccgg cgccttgcgc gacctcggga tgagccagag cgagttcgat
     1081 tcctactggg ccgagtccca cgggctggcg gaacccacgc gcgtgcgcgt gatcgagggc
     1141 gttcgccgca ggtccgggcc gtgaacgccg caggatggcg gctgacgttg gcagccgccg
     1201 tattgatggg cctcgtggtg ggcgggcgca gcgccttcgg cctcttcgtt tcgcccctga
     1261 acaccgcttc gggcatgggc ctcgccgcgc tgagctttgc gctggcgctg gggcaactcg
     1321 gcattggctt cgcgcagccg gtgatcgggg cgttggccga ccgtcacggc gcggcacgcg
     1381 tgatcgtcgc cggtgccttg gtgctggcgg caacgctggc ggtgccgtcc gtctggcctg
     1441 tgccgatggc ggtgtcgctg gcgctggtgt ccagcgcagt cgccggcagt gccgtaggca
     1501 gcaacgggct gctgctgggc gaagttgcgc gggcggtttc gcccgcgcgt gccggactgg
     1561 ccgtcggctt ggtcggcgcc ggcgcgtccg ccggccagct cgtcctgggc cccgccaccc
     1621 agtgggcaat cgacgagcag ggttgggccg tcgcgctgct ggcgacggct gcgctgagcc
     1681 tgatcgcctt gccgttggct attccgttcc ggcgccagtc tcccgtcaag gcagcgcagc
     1741 cgtcccagcc ggtgggcgac gtgctgcgcg agtggcggtt ctggcgcgtg gcggcgagtt
     1801 tcggcgtctg cggcttccac atcgcctttc tcgccgtcca catgcccggc gtgatcgagc
     1861 gctgtggatt accgccgtcc ctggcgggaa cctggatcgc gctggcgggc gctgccaaca
     1921 tcgccggcag catcgcggtc ggcttcgccc tcaagcgcca cgatgcgggc gtcttgctgg
     1981 gcagccttta tgcgacacgc gccctcggga tcgccgcgtt gctcgtgctt ccggtgacgc
     2041 cgatgctgat gctcggcttc gcggtcgtca tgggcgcgag ccacatggcg acccttccgc
     2101 ccaccgccca actcgttgcc cgccagcacg gcgtgcaacg gctgggcact ttgtttggca
     2161 tcgtgatgct ggtgcaccag gtcggcagct tcgccggcat ctggctcggc ggctgggccg
     2221 ttgaagccac gggcagcgac aacctgctgt ggtgcgtgga cgcgggcctg gccttgtgcg
     2281 cagcggcgct ggtgtggccg cgcccgaggg ctgcggaatc cgcatccgtc ccaaggttgc
     2341 gcgccagccc tgtgcccgcc cgcgccactg cgctggcgca acgccggcgc attccttgaa
     2401 cgcgcgactg cacgccgcgt gcggtttcgc aaggacagaa gcctcatcga accggcgctg
     2461 ccggctgcag ccacgccggc aacaattccg aggggtcacc gatcgcttcc gcctggaacg
     2521 gtacggagtt ccgtatcagt agcgcctgca gccttccctc gcgccatgcg gccagcgtgt
     2581 ccatgcagcc gcccaaccat tggccgccga cgaagacctg cggaatggtc ggctgcccgg
     2641 tgtgcgcgtg cagagcagcg cggatctgca ggccgcgccc ttccgcgtgc caggcagcgg
     2701 catcgaggtc gatgctgtgc aagggcaccc cgattcgctt gaacaaccgc cgtatcgtcc
     2761 agcagaactc gcaccactcc agggcgaaca tcactaccgg gcgcgccggc tcgatcacag
     2821 cacgttcgac gaaatcacgt ccccaggggg tgacggcggg caacggggcg ggagctggcg
     2881 ccggggtggg ggcggcgggg gacagtgcgt cgaagcgcgc gcgcggcgtg ctgcgggaga
     2941 tagcccattc ttcggcgttc atttcggaat cgatgtccga gaacagcggt gtgctgaggt
     3001 agcgctcgcc cgtgtcggga agcatcgcca gcagtcggct gcccggcggc agttcggatg
     3061 ccaacagcag cgcgccggcg acggtcgcgc caccggagat gccggccagg atgccttcct
     3121 tgcgcgccag ttcgcgcgcg gtttccaacg cgcgggcgcc gggcacggct tgcacgcggt
     3181 cgatgagtcc ttcctcgcgc gcccgctgca ccaaggccgg cacgaaatcg gtcgtccatc
     3241 cctgcatcaa gtgcgggcgg aagtgcgggt ggctggacgg tgtgccgtcc gtcgccggcg
     3301 gcggggccgc gatgccgctg cccagaacag gcgcgttgtc tggctcgcag accacgattc
     3361 gcgtctgtgg cgagtggcgg cgcagcgcgc gccccacgcc gagcagggtg cctcccgtgc
     3421 cggcgcccgt gatccagtgc gtcagcggcc tgtcgtggaa gtcctgcacg atttccagcg
     3481 ccgtcgtgcg ttcgtgcgcg gcggcattgg cctcgttgtc gaactggctg ggcaggaacc
     3541 atccgtgcgc ctgggccagc tcacgcgcct tggccaacat gccgctgccc ttgaggcccg
     3601 cgggcgtcag cacgacgctg gcgcccagga agcgcatcag ccgccttcgc tcgacgctga
     3661 agttttccgc catcacaatc accagcggat agcccttgcg ggcgcagacc atggccaggc
     3721 cgatgccggt attgccgctg gttgcctcga ccactgtctg gccgggcacc aggcgcccac
     3781 tggcttcggc atcttcgatg atggcgagcg ccatgcgatc cttgacggag cccatggggt
     3841 tgaatgcctc gagcttgacg aacagctcta tgccggccgg cgcaaggcgg gagagccgga
     3901 ccacaggggt tcggccgacg gtctgcagga tgtgctcgtg cctcatgaga ttcctttcgc
     3961 gccgcccatt gggaggctgg ttgcggaaaa ggaagtctcg acggcggccg tatttacgag
     4021 atgtcagcgg gccgccaatg tgtatcccgg cggaaatgcg atggaaaacg gccccttcaa
     4081 gcgaaggcgg cgggaacttt ctcggtgcca ggtcgctcga gcagctcgcg cagcaacgag
     4141 cgcagcgcgt cgctgtcgat cggcttgcgc agcagggcgc tcacgccgca ctcggccagt
     4201 tccgcctgcc cgatttcaga cgcgttgccc gtgtacagca gcaccgggag cgcaggcctg
     4261 tgccgtacgg catgcttcga aagcgccagg cccgtcaagc ccggcatggt ctggtcggtc
     4321 agcagcaggt cgaacacctc ctcctccgat gccaaccgcc gggccgcggc cagcggatcg
     4381 cgctccagcg ccacgtcaag tccccacccc gtcatcacgt ccatcatgaa atcgctcacg
     4441 atggactcgt cctcgaccag cagcacccgc ccgcgaagac tgggcgctgc ccgcacgacg
     4501 ggtgcgggcg cggccggagg atcggttcgc cgtgtgtcct cggccgcggc cggcaacagc
     4561 acccggaaga gcgatccctg gcccggcgtg gaaacgacct ggatgtgccc cccgtggtca
     4621 tggacgatac cgtggaccat ggccaggccc atgccggtac cccggcccac ttccttggtc
     4681 gtaaagaagg gctcgaacat ccgatccatc acctcgcgcg gcatcccgcg gccgtcgtcc
     4741 gccacctcga cccagaccca gcgactgtcg tcgagcggcg cgctgcatga agcgcaatgc
     4801 cccgccgttt ccgagtagcc gatgcgcacg cggatggtcc catgatcgcg gatggcgtcg
     4861 cgcgcattga tgcacaggtt gaacagcacc tgttcgagtt ggaccgggtc ggccacgacg
     4921 ggcggcactg ccgggtcggt atcgctgcaa ataccggggc attcgacggc gatcgacgag
     4981 ggcatgttcg ggcgcagcaa ctgcagcgcc tgtcgcgcga ccggcgccgg cgccagcagg
     5041 cgcctttcgc cgcgcctggg acgagagaac gccaggagtt gcgccacgtg gtcgcgcgcc
     5101 cgctcggcgg cgaggcgggc ctgccccagc tggcgcgcca ggaccgggtc ttcgcccgcc
     5161 gtgggtcgct cctgcgccat ggtgacgtag cccagaacgc tggtcagtat gttgttgaaa
     5221 tcgtgcgcca gcccgcccgt gagctggccg atcgcctcca tcttctgcgc ctggcgcagc
     5281 tgccgctcga gctcgcggcg gcgttgttcg gcggcgttgc gctcggtgac gtcacgcgag
     5341 gcatacagca catgcggccg gccgcggtac atcatcggcg tgccgcgcac ctcggcctgc
     5401 attgtcgagc cgtccttgcg cgtggcggtg acctcgaatc tcagctcctg gcctgccaga
     5461 gcgagttcgt gatcggagcg atgccgggcg cgcacttcgc catcggcctg cgtgagcacg
     5521 tggttcgcgg ccatcacctc ctcgcgggtg tagccgctca tcgtggtgta ggccgggttg
     5581 acctcgacgg cgcggtagtc ggcgtcccgc agcaccagcg catcggccga ggcgttgaag
     5641 atcgcgcggt actgctcctc gctgtcgcgc agcgcgcgtt cggcactctt ggcttgcgtg
     5701 atatcgcgcc ccatgtacag cacatgcggc tcgccgcgat gctggatggc cacgccgcgc
     5761 agttccaggt cgtagcgccg gccgtcgcgc cggaacatct gggtttcgag cgaaaccgcc
     5821 tcgcctgcga gcgccttttc gtgcagggca cggatcgtcg ccaccacctc gggcgggttc
     5881 gccagcacac ggttcacacc cagcacttcc gcgcggctat agccgctcat cgcctcgtag
     5941 gtcgcgttga cgtcgacgat tgaaaaatcg gccgcgcgca gcaccagcgc gtcggccgag
     6001 gcgttgaaaa tcgcgcggta ctgctcttcg ctgtcgcgca gcgcctggtc ggcacgctgt
     6061 cgctccgtga tgtcgcgcag atggcccacg aagatgttgc cctcgggcac gaccgcgacg
     6121 ctcatggcca gttcgaccgg gatttcgctg ccgtcggcgc gcagcatctt ggtttccacc
     6181 agccttccgg ccatgggccc tccgccactc gcgcgcaaat ttctcatctc gcgctcgtag
     6241 gcatcgtgat ggcgcttggg cagcatcatt tcggccaggg gccggccgaa cacgtcttcg
     6301 cggcggtggc cgaacacccg ttcggcggcc gcgttgaatt cgacgatgag gccgtcgctg
     6361 tccatgccca cgacggcgtc gaatgcagcc tcgaccgtgg cgcgcaaccg ggcctcgctg
     6421 cgctgcagtt cgcgttcgcg ccggcgccgt tcgctgatgt cgcgcaccac ggcaagcgcg
     6481 tgcgcctggc cccgctgaac gaagggcaag acccgcagat cggcgtcaaa cgtggtgccg
     6541 tccggccgca ggacggtggt ttcgacgtgc gtcgtttcgc cggccatggc gcggcgaacc
     6601 atctgcaacc gttccctgac gtacgcctgc ggcatggtct tcgggaaagt gcgcccgatg
     6661 acgtcctcac ggcgatagcc atacagcgca agcccggccg ggttgacgtc cacgatgcgc
     6721 aggtcttcgt cccacatgaa caggccgtcg agcgaacttt cgaagatggc gcggtattgc
     6781 tcctcgcgca ggcgcaggtc ttccaatgcc tgcttctgcg cggtgatttc gcggctgaat
     6841 gccacgatgt gcgggcgccc gtcgatcatg gccggcttga gccgcacctc gtcccaatgc
     6901 aggctgccgt ccttgttgcg ccgctgccat tcgaacggcg ggcagcggcc cagcttggcc
     6961 gactgcatga ggcgcagcgc ttcctcggcc gtgtagggga atacgcccga gctggtatcg
     7021 gccatcgtgc tgcggcgcag ttcctcgaag gtgtagccgt agatgtcgca ggccttgcga
     7081 ttgacgtcga gcaccgcgcc cgtttcccag tcgtggatga agaccgcgtc ctcggaggcc
     7141 tcgaagatgg cgcggtagct cgcctcggaa cgctgcaatg cctcgctggc ccggagcctc
     7201 tccacctcgg cggcggcccg aaccgcgaag atcttgagca tcgcttccac acggtccacc
     7261 tgcgccggcg ggatcgggcg ccgcgatgcc accgacacga tgcccagcgg cgagccgtcc
     7321 agcgcaacga gcgggtggcc cgcgtagctc acggtgcatt gctcgcgcgc gtcctggtcg
     7381 tcggggaaca gcgactgcag gtcggcaggg taggcccgga agcgttgccc cagcaccgtt
     7441 tcgcaaggcg tgccggcgag cgggtaagtg atgtcctgca cgacctgccc gtcgtagtac
     7501 atggccagca tccgaagcga gtttggccgc gccggctcgt agcgcgcgat gaaggctacc
     7561 tcgacatgca ggatggtggc cagcaggcgt acgagctcgt cgaagaccgt cccgctgcgc
     7621 gaacccgata ccgccagcgc caccgcgcgc agcgcttcat cggtacgctc gcgctcgatt
     7681 tccgccgcca cgcggccggc gatgatcttg agcatcgcct ccgcatggtc ggcgtcaccc
     7741 gcggcgatcg gccggcggtc catcgccacc accagcccca gcggctcgcc cgcgctgtcg
     7801 ttgagcggat acgccgcgaa ggaatccatg cgcttcctgg cgctcatcga ctcgggcgcg
     7861 agccgtggga gaaggccgga ctcgatgaac tggcatgacc gtccgaccac cgcggcgcaa
     7921 ggcgagcctt cgagccggta atcgaaattg cgcagcaagc gcccgtccag gcatgccgcc
     7981 agggtgcgca tgacggtgcg cgaatcgtcg gcgaacaccg cgatgaagac caggcccacc
     8041 tgaaggctgg ccgccagttc ccgcgcgagg tccgcgaaca ggttcggccc gcccgggtgg
     8101 gccactgcca gggcgacccg gcgcaaggcc tgcacctgag tcagcggaat ggcctcggcc
     8161 tcagtgggcc cggtaggcga cgcttgactg ctcatggccg cagtctacgg cgcagttgcg
     8221 cgccccgggg ccggaatttc cttgcggata cacggcggat acatggcggt tgcaaaccgg
     8281 cgcgccggtc gcaaaccggc taccaggacg gggcgccgcg aagcagccgg gttacctgcg
     8341 cgggcgatgc ttggcacctc gctcgataag agcggcattt ccccaggagc cagccaccat
     8401 gtccatcatt cgccacctgt tcggaaaccg ttcgcggtcg acccccgagg tcgtgatcgc
     8461 gctccattgc agcggcagca gcggccgcca gtgggatgcg tacgccaagc tgctgccgcg
     8521 cggaatgcgc ctggctgcgc cggaactgat gggctactgc ccgcgcgagt gctgggagtg
     8581 cggcactccg gtcagcctcg aagcggaagc gcgcaggctg ggcccgctgc ttttctcgca
     8641 caccggaggc gttcacctgg tgggccattc ctatggcggc gcggtcgcgc tgcagatggc
     8701 cctgcgctgg cccgatcgcg tcaagacgct gacgctgttc gagcccgttc gcttcgctct
     8761 gctgctggaa gatcacgatc aggaagccac ctggaaggcg attgtcagca tcggccgccg
     8821 catcggctgg cacgtgctgt cgggccgcct cgacgaagca gccgcgcttt tcgtcgatta
     8881 ctggtcgggg agtggcgcgt gggaacgctt gtccgctgcc cggcggcgcg ccatcgccga
     8941 gcggatgccc aaggtgcggg cggagttcga ggcgctgttt gccgacacgg cacccgcctg
     9001 gtggttccga aagctggaaa tgcccgtgcg gctcatcact ggcagcacgt cgccgctgcc
     9061 ggcgcgcagg gtcgccgaca tcattgcccg ccattgcgcg caggccgaag tcgttcggct
     9121 ggagggcgtg ggtcacatgg gccctatcag ccatgcgccg ctggtggcgt ctcacctggc
     9181 gttccaggcg cgcgatgcgc aactcgcgct ggccgcctga ggtgacgcca tgagctccgg
     9241 atcagcaaga ctgtttgccg tcgtgcgatg cgtgccggct tctgaggaaa agaaactaaa
     9301 gctcagcccg ggcgcccgcc cgatcccagt tgagctaacc cgacacgatg aagtcaggat
     9361 tgaacatgcc tgcgtgcgcc aaatcgaaca aggatcgaca atggacatca tgattcgctg
     9421 gtttccttcg cgtgaaagcc tgatgcggtc ccgctggctg gggccgctgg cgcaccattt
     9481 ccaggatgaa agcctgtggc aggtggagcg cggcagcatc gcacgcgggg tggcgatcgg
     9541 cctgttcttc gggttgctgc tgccgttggc gcagttcctg ttcgccgtgg cattcgcgat
     9601 cctgttgcgc ggccatgtgg ccgtggcggc cgccgcgacg ctggtttcga acccgctgac
     9661 attcgcaccg ctgtactggt tggcacaccg catcggaagc gcgttgctgg gcagctccag
     9721 ggcggaggcc agggtgcatg ccgccatggt ggaggcgcag gtggaagtca ccgccgctgc
     9781 gcaaggatgg ctccgcgcca tctgggaggc ggtactgaac gcgggcgcgc ctctcctgct
     9841 tggcctggcc gtgatggcgg tggccggagc agtgctcggc ttcctggtgg tctggctcct
     9901 gtggcggcct cggcgcgacg gcaggccaaa cgcggggtaa gaaagcaaga cgctggcggg
     9961 cttccgcccg cccccgattt cgtacctaac ctgatcgcca cgatttacac tgccgcatcc
    10021 gaatcaattg cgggaaagat ccatggcgcc gttcctatcc cgccttgcgc gagacggttg
    10081 cgccggcaag gcatttgtgc cggcactgaa ccttcttgtc gccgtactgc tgtcgttgac
    10141 aggaacgctg gcccgcgccg cgctgccggt gctggcgccc tcgccgttcg ccgatggcac
    10201 gcaggtcgat acggctgtgc tgggctaccg cattcccgtc atcctcgttc acggcctggg
    10261 agggtccacc gagggctggg accgttttct gcgggcttac gagcaaagcc cgggctggcg
    10321 cgatgcgttc aagccttact cgtttcgctt cggcagcacc accgcggagg tgatcttcga
    10381 cccggcggcg ccgcgcacga tcagcgccct gggcgcggca ttgcgcgacg ccatgcaggg
    10441 cttctacgac aggccggagg ccgcgccgga cttcgggttc ggcaacaagc gggtgatcgt
    10501 cctggcccat tccatgggcg ggttggtcgc gcggtcgatg atgcaggaac acgttttttc
    10561 agacggccag cgcggcgggc agaaagttct gcacctgatc acgctcggaa ccccgcacca
    10621 cggcactcca ctcgccgacg cggccatcgc gctgggcctg cacacggtga gtgaactgga
    10681 cgagggttac ttcgctttcg tggccgatac ggcctggacg aatttcgacg gcctgaacat
    10741 gtccggtgga cgctgcaacc actggctcgc gcagttgaac aactatgcgc ccgccaccgg
    10801 cggaaattac ggccgttgcg gtgccgtccc ggccaatccg ctgccgggtt actactggaa
    10861 aatcatcgcg tacggcgcga gcgccttgca gaaccgggac tggtacctgg gggagaccgg
    10921 cgtttacaaa cccggctcct ccacgtccct tctcttcccc tacaactacc tgcgcggcgc
    10981 actgccgcgc agctacccca atgatggcat cgtgcccctg gtcagcgccc agctggaggg
    11041 agcttcgctt tggctcaggc gccaagcgta cgactgcgat cacaggtaca tcaggagggg
    11101 ctaccccgaa ttcgtcagat cgccggcgtc cacctacacg gattgggcgt tttgcgcggg
    11161 caccgacagc ggccaaagtt atgcctcggg catttcgggg ggctatgcgg tgtccggctc
    11221 gatcatgggc gtggccggcg gcatcgtcga tacgatcaag accgcgtcgg agatggaacg
    11281 cgtcttgaac tgggtcgagt ggaagtttcc gggctacctt gccccggcgg gcgccacttc
    11341 cgacatcgtc ggcaagcact attaccggta ctactcgtca accgaggctt atctcgccgt
    11401 gagtagcggc aatctttatt acctgggcgc cgcctcgggc agcccgcttg ttttcctggg
    11461 aacgcttggc gatctcctgc ctcatgcgca ggcggcggga ttttagggcc cgcatgcatt
    11521 tgcttcgccg ccatctgatc ggcctcctcg ccggccttgc cgcgctggtc tgcgcccacg
    11581 cgcccgccca gacggacgag gaagtcccgt tcatcacttc gcccgacgac gtcacgctgg
    11641 aaatgctgcg catcgccggc gtggcgccgg gtgaccatgt gatcgacctg ggctcgggcg
    11701 atgggcgcat cgtgatcctg gcggccaggc gcttcggcgc gacgggcctg ggcgtggaga
    11761 tcgatcctga actggtgcgc aagagcatgc ggcacgcacg cgaggcgggt gtggcgcagc
    11821 gcgtgacgtt ccgcgagcag gacctgttcc agaccgacct ctcggcggcg agcgtgatca
    11881 ccatgtacct tctgccgcag gtcaacctgc aactgcgccc cgccttgctg gcgctgaagc
    11941 ccggcacgcg catcgtgtcg cgcgattggg acatgggcga ctggaggccc gaccggacga
    12001 cggtggtgcc cgtgcaggac aagaaggtcg gcctggacaa atcgagcaag gtgcacctgt
    12061 gggtcgtgcc ggcgcgggtg gaaggcctgt ggtgcggcac cgggctgctg cgggaattca
    12121 gcctgcagtt gtcacagctg taccagcaag tgaacggcag cctcgcgcgg cgggatcgcg
    12181 cccgcgccat cgaaggccgc atcgatggcg ccaccgtgcg cacccaggcg acccgccatg
    12241 gctcgctggt actcgctctg gtgggcgatg agctgagtat caccggcggc gaaggtactc
    12301 tcgcgctggc gcgaggcgcc gcgttccggc gcgcggccga ccggggttcc tgcgacggct
    12361 gaagaaccgc gcgcagtcag ctgctctggc cgcctcccac catccagggc gtgccgaacc
    12421 ggtccgtgag catgccgaac gcctcggccc agaaagtctt gcccaacggc atgattacct
    12481 tgccgccgtc ggccagcgtg tcgaatacgc gccgggcttc gtcggccgat gcatagttca
    12541 gtgagagcga gaaaccgttc atgcccgcac tgggctggtt cgccggaatg tccgacgcca
    12601 tcagcgagcg gccatcgatt ttcaggctcg catgcatgat gcggtcctcc gagccggccg
    12661 ggcagtgttc gggctccggc gactggccat aggtcatcat catttcgagc ttgccgccca
    12721 gcgcggtttc gtaaaagcgc atggcctgcg cgcagttgcc gttgaagaag aggtagctgt
    12781 cgagtacggg catgagatat ctcctggttg ggtggtcagg acgggccccg caggtctttg
    12841 cgcggaaatc ccgctaaccg aatctacgac gaaccaggca cccggaaatc gacagaatgt
    12901 gcgcgaacct catcggcgcg cgactcgaca gctgcgggaa atccggcagc tgccgagccg
    12961 gctatcactc acgcctcgcc ccgaactgat gcatagccgg tggcctacac gcgcgttgtc
    13021 tcacggatgg ctcacacaca gcctggcgtc ggacggctcc aaccggttgc acgctgcaaa
    13081 gttgctactt cacgcaaggc aacaagcagc atcgcatgag tgccggacct tttgcagaaa
    13141 tgctcttgct ggcggccgca ctggcggcgt cgctggtggt gcggccgtgg cgaatgctgc
    13201 gcggggccga ctccggctcc gggctcgcca cgccgctgct cgcggcgctg acgcttcttc
    13261 catggctctg ggcgtggccc tccagtgctg ccatgccgat tccaatacag tggtccggcg
    13321 cggcccttgc ggtactgatg ctggggtggc cgctggcagt tccggtgctg gtcgcggcgg
    13381 gcatcagcac ggtgttcacc accggcgcgt catggcccgt ggcactttcc accatcgtct
    13441 ggtcgggctt gctgccggcc accatcgtgt tgctgctggg ctatgcgatg cgcagggtgt
    13501 tcggcaccca tcccgccgtt tacctgctgg gccgcgcctt cgccgtgccg ctgctggcgc
    13561 tgttcggctg tgcgctggca ggggccatga caagcgggac ccttgcgggc gaggatcgtg
    13621 aaatgcaggt agtggccgcc ttcctgatgg cgatggccga aacgacctgg acctgcgccg
    13681 tcgcctccat cctggtggcc tggaggccgc aatggctggc cacctggtcg gatgcgctct
    13741 acctgggtcc gcgccgtcgc gcatagcaac atgccggcgc ggcggcgcca gtcttcgccg
    13801 actgggcgcc cggccctcgg cgacggacaa atggagtaaa acacgcccat gtccgcgtcg
    13861 ccccgcatca cccgccggtc cttgctcctc gccggcgttc ctgcttcgct gttgcaagcc
    13921 gggtgcgccg ccggccgatc ggcgatcacg ccgcggcagg tggaactggc ggccgcgccg
    13981 gcacaggttt tcttgcgcgg cagtgcacag ccgccagtag cggcctggag ttacggtggg
    14041 tccgtgcccg gcccggaaat ccgggtgcgg cagggcgagc gcctgcgcgt ggtggtgcac
    14101 aaccggcttg cccaggggac aaccgttcat tggcacggcg tccgcgttcc caacgcgatg
    14161 gacggggttc cccacctgac gcagccaccc atcgaacccg gcgcccggtt cgtgtacgag
    14221 ttcgacgtac cggacgcggg cacttactgg taccactccc acctgcacag cgcggaacag
    14281 ctcgaacgcg ggctgtacgg ggcgttcatc gtgcaggagc cacagccgct cgaggtggac
    14341 cgcgacctca cctgggtgct cgacgactgg cggctcacgc gcgaggggcg gctcaatgag
    14401 tcgttcaatg acccgcacga tgtcgcgcat gccggccggc tgggcaacgt ggtcactgtc
    14461 aacggaagtt ttcctcgaga cctcgcgctg cggcgtggcg agcgcgtccg cctgcgcctg
    14521 atcaatgccg ccaacgcgcg catcttttcg ctccaattcg acggccaccg gccgcgggtc
    14581 gtcgccatcg acgggcaacc ggtcatgccg cacgagcctg cgggcgggcg cgtgacgctg
    14641 gcgccggcca tgcgttacga cctcgtgctg gacgcgatcg gcgagccggg aaaaagctat
    14701 gcggtgggcg acgtcttcaa cccgcgtgcg gcgtatgacc tgctgcaact ggcttatggc
    14761 ccaacggtgt tgcgagagca attcctggag gcgccggtcg cgctggctcc caacgcgctg
    14821 ccggagcctg acatcgacag cgcactgcgc catgaaatcc gttttgaggg cggcatgatg
    14881 ggccggatgc accaggcgtt gctcaatggc aagccgctga acatgatggg gttgctgagg
    14941 cagggcaagg cctgggccgt gaatggcgtg gtggccgatg ggcatgacat gaaacccatg
    15001 ctgagcctgc agcgaggccg cagctacgtc ctggcgctgc acaacgacac ccgctggcac
    15061 cacccgatgc atttgcatgg ccacagcttc cgggtgatcc gccgcaatgg ccaacccacg
    15121 cgttaccggg aatggcagga cacggtgctg atggaccccc aggagcgcgt ggacatcgtc
    15181 ttcgtcgcgg acaaccccgg cgactggatg ttccattgcc acatcctcga gcatcaggac
    15241 ggcgggatga tgagcgtcgt ccgcgtgtcg gcctgatcgg cctcgcggca gctcgcaacg
    15301 ggtccccgcc atcaagcccg gaactgtttc gccacgaagg cccacaacat ccgcgacgca
    15361 tcgggacctt tggcgtcgct gtgggcacta ttatcggcct cgaaagcacg tgagccatgc
    15421 aaaattgcag gacctgcctg gagagcgcac catgcccgaa aaagttcccg ccacgctgat
    15481 tcccggtgat ggcatcggcc ccgaaatcgt cgacgccgcc ctggcggcgc tggacgcgct
    15541 gggctcgccc ttcgagtggg accgccagac cgccggcatg gccggcgtga aagacagcgg
    15601 cgacccactg cccgcggccg cgctcgccag catccgccgc acacggttgg cgctcaaggg
    15661 cccgctggag acgccttcgg gcggcggctt ccgctcgtcc aacgtgcggc tgcgcgaaga
    15721 gttccagctc tatgcgaacc tgcgaccggc gcgcaccatc atcccggggg gccgcttcga
    15781 caagatcgac ctggtggtgg tgcgcgaaaa tctcgagggg ctgtacatcg gccacgaaca
    15841 cttcgtgcgg gtggacgacg atccccatgc ggtcgcgatg gcgaccggca tcaatacgcg
    15901 gcagggcagc cggcgcctgc tcgaattcgc gttcgatcat gcgttggcga ccgggcgcag
    15961 gaaggtcacg atcgtgcaca aggccaacat cctgaaggcg ctgacgggga tgttcctgga
    16021 gaccggcgtc gccctttacg agcagaacta caagggacgg ttcgaactcg acaccgtgat
    16081 cgtcgatgcg tgcgcgatga agctggtgct caacccctgg cagttcgaca tgctggtcac
    16141 caccaacctt ttcggcgacg tgctgtcgga cctggtcgcc gggctggtcg gcggcctggg
    16201 catggcgccc ggcggcaaca tcggatgcga cgcggccatt ttcgaggccg tgcacggctc
    16261 ggcaccggat atcgccggca agggcatcgc caacccggtg gccctgctgc tggcggccgc
    16321 gatgatgctc gaccactgcg cttcgccgga gctggccgca cgcttgcgca aggccatcgg
    16381 cgagacgctg aacatcgacc aggtgcgcac cggcgacctc ggcggtcatg cgaacacggc
    16441 cgcgttcacc aaggcactgg tgagccgcat cg
//
LOCUS       DASWIY010000280         6368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2043712, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000280 DASWIY010000000
VERSION     DASWIY010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043712"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VGA59_10290"
     CDS             <1..257
                     /locus_tag="VGA59_10290"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEX9720109.1"
                     /translation="YLACTEATVPTCVGHWTSVVSAKVFLLSRNVEAQGGSADTNKYV
                     LGRKADGTPNEYTSLPAGYKRKVFQEVVRLQNPSGRRATP"
     gene            254..844
                     /locus_tag="VGA59_10295"
     CDS             254..844
                     /locus_tag="VGA59_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720110.1"
                     /translation="MKRQGQRGITLVISLIMLVLLTIMALTSFNIGKSSLQVIDNAQQ
                     QGQVLNAAQTMLNQVVSSPIFTEAPDNVLDNSNCPPALAAPANSRCMDLYGDGKTVIV
                     VAMTPQPVCVQATPVPATALNLDTQNSEDWGCTAAIRTDHGIEGLETGDSLCANSLWQ
                     LNADASEPVSGARAVVTQGVAMRVSLDSVDTTCPAP"
     gene            892..4032
                     /locus_tag="VGA59_10300"
     CDS             892..4032
                     /locus_tag="VGA59_10300"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HEX9720111.1"
                     /translation="MFCSPWIAIRNSLAGVLALAAVLAAPAVFAEDIDLFVVRDAESA
                     AAPNLMIMLDNSANWARQSQQWPDNGGAQGAAELQALINILSSPLNANIGLTFYNKVG
                     NENGGYIRFGVRDVSGSTAGTANRTALVNILGLMKANINDPIEKVNDNNGEAAALYEV
                     YKYYKGMRPFRGSVSADTPNADKGNNSGSSAGRTAFGQGLTSDFALKSNGDYLGAPVN
                     TCGRNYAIFIVNNAQGTIPPGQQTYESTSAGTSLGPIQGVTDVSWTDEWARFLYNNGI
                     SVYILDAYYAQQNVSHSSVLKRAADIAGGRYIKVSKQEDIQLAIEDILAEIRAINTTF
                     AAASLPISATNRSQNLNQVFIGMFRPADTNKPRWMGNLKQYQLGTSASGVDLVDRLGM
                     NAVNSQTGFVSECAVSLWTTGSGTYWQNVTANRLARSGCVNFPTVDGVLGAEWSDSPD
                     GATVEKGGVAEILRKGNNPPATDTSPTWTNNRTILTYSSATATKLTPLTTAATGWTST
                     LHDWVTGIDDPAVGYTEFIDTSVSARTRASIHGDVIHSRPLPVNYGGTTGVTVYYGTN
                     DGMLRAVDAATGKERWAYVAPEHYANYQRLYDNSPVVTFQNADPTSSATSKKYFFDGS
                     IGVYQKADNSQVWIFPSMRRGGRMLYGFDVTNPASPILKWRVGCPNQTDNTGCTSAEF
                     EAMGQTWSLPSVAFLKPYDLNAAKIPVVVVGGGYDGCEDEDVPIPTCTGRKGKAVYVL
                     NADTGAVIKKFVPTEDAGSFAADVAMADANGDGVVDHAYAVDTRGNIYRIDFSSDSNE
                     PAAYGSWTMRRVAYTSGAGRKFLYPPSLLRVANKMYVALGSGDRERPLLGNYPWDTNV
                     VNRFYVLLDDLTIRDAAAPAVPLDTDPGMKDFTTLASTSCDESGVLPTSGLKGWFMSL
                     PNRGEQVVTSALIAAGMVAFNTNRAVPSAEAAACVAPLGEARGYWLNLVNASGAIAAG
                     NKTCGGDRSSVFVGGGLTPSPTLARVVIGNRVTTVAIGAANRDGGVSVPIDPQEVKPV
                     ISSKRRTIYWKSNAAD"
     gene            4141..4746
                     /locus_tag="VGA59_10305"
     CDS             4141..4746
                     /locus_tag="VGA59_10305"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEX9720112.1"
                     /translation="MDTPNNKPESRRSRSTHHGRASRRPLGFTLVELISAVGVMAILA
                     ALAMPSMARIVDSTRLTAISNDFLSSVYLARSEAVKRNRPVGLCKSATGVSCTSTGGW
                     EQGWIVFHDPNNNGSADAGEAVVHHTQALPQGFKLSGNLNVAKYISFTPFGRTRMVSG
                     AFQAGTLTLCKANGTEIGAREIVINHVGRARVNKRSISACA"
     gene            complement(4759..5208)
                     /gene="nrdR"
                     /locus_tag="VGA59_10310"
     CDS             complement(4759..5208)
                     /gene="nrdR"
                     /locus_tag="VGA59_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX9720113.1"
                     /translation="MKCPFCGNAETQVVETRMAEDGDFIRRRRQCLGCEKRFTTYERP
                     DVSFPAVVKKDGRRIDYERAKLQASMNLALRKRPVSTEQVDGAVERIEEKLLNLGVRE
                     IPSARIGELVMRELKKLDKVAYVRFASVYRSFEDIDEFKTLVDEVRR"
     gene            complement(5224..>6368)
                     /gene="glyA"
                     /locus_tag="VGA59_10315"
     CDS             complement(5224..>6368)
                     /gene="glyA"
                     /locus_tag="VGA59_10315"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX9720114.1"
                     /translation="SENYASPAVMAAQGTQLTNKYAEGYPGKRYYGGCENVDVAEQLA
                     LARVKQLFGADAANVQAHSGAQANEAVFLAFLKPGDTIMGMSLAEGGHLTHGMALNMS
                     GKWFNVVSYGLDEKEEIDYDAMERKAHESRPKLIIAGASAYALRIDFERFAKVARDVG
                     AIFMVDMAHYAGLIAAGVYPNPVPHADVVTSTTHKSLRGPRGGFILMKPEHEKAVNSA
                     IFPGLQGGPLMHVIAAKAVAFKEALAPEFKVYQQQVARNAQVVAQTLVERGLRIVSGR
                     TESHVMLVDLRAKGITGKEAEAVLGDAHMTINKNAIPNDPEKPMVTSGVRIGTPAMTT
                     RGFREEEASLTANLIADVLDNPRDAATIAQVREKVNALTRRFPVYG"
BASE COUNT         1186 a         2062 c         1981 g         1139 t
ORIGIN      
        1 tgtacctggc atgcacggaa gcgaccgtgc cgacctgcgt cggccactgg acgtcggtgg
       61 tttcggccaa ggtgttcctg ctgagccgga acgtcgaagc ccaggggggc tctgcggaca
      121 ccaacaagta cgttctcgga cggaaagccg acggcacccc caacgagtac acgtccctgc
      181 cggcgggcta caaacgcaag gtcttccagg aagtcgtgcg tctgcagaat ccgagcgggc
      241 ggagagccac accatgaagc ggcaaggaca gcggggcatc accctggtca tctccctcat
      301 catgctggtg ctgttgacca tcatggcact cacttctttc aatatcggaa aaagcagcct
      361 ccaggtcatc gacaatgcgc agcagcaagg gcaggtcctg aatgcggccc agaccatgct
      421 caatcaggtg gttagttccc cgatcttcac ggaggcgccg gacaacgtcc tggacaactc
      481 caattgcccg cccgcattgg ctgcccccgc caacagccgc tgcatggacc tctacggcga
      541 cggcaagacg gtcatcgtcg ttgccatgac gccgcagccg gtatgcgtgc aagccacgcc
      601 tgttccagcg acagccctca acctggacac ccagaatagt gaggactggg gctgcacggc
      661 ggctatccgg acagatcacg gcatagaagg cctcgagact ggcgactcgc tgtgcgccaa
      721 cagcttgtgg caactcaacg ccgacgccag cgaaccggta tccggcgcaa gggccgtggt
      781 gacccagggc gtcgccatgc gcgtctcgct cgactccgtc gacacgacct gcccggcgcc
      841 gtgaagccca agcccgccga tcgaagaata tcccgaggaa gaaaggcaat catgttctgc
      901 agcccatgga tcgcgatacg gaactcattg gcgggtgtgc tcgccctggc tgcggtactg
      961 gccgcgccgg ctgtttttgc cgaagatatc gacctgttcg tcgtcaggga tgcggagagc
     1021 gcggccgcac ccaacctcat gatcatgttg gacaactccg cgaactgggc ccgccagtcg
     1081 cagcagtggc ccgacaacgg aggcgcccag ggggcggcgg agttgcaggc cctgatcaac
     1141 atcctgtcga gccccctcaa tgccaatatc ggcttgacct tttacaacaa ggtcggcaac
     1201 gagaatggcg gctacatccg gttcggggtc cgcgacgtga gcggcagcac ggccggaacg
     1261 gccaaccgca cggcgttggt gaacatcctg ggcctgatga aggcgaacat caacgatccc
     1321 atcgaaaagg tcaacgacaa taatggcgag gctgccgcgc tttatgaggt ttacaagtac
     1381 tacaagggaa tgaggccgtt tcgcgggtct gtgagcgccg atacacccaa cgcggacaag
     1441 ggcaacaatt cgggcagttc tgctggacgg acggcatttg gccagggact gacttcagat
     1501 tttgccctga agtccaacgg ggactacctg ggcgcccccg tgaatacgtg cggccgcaac
     1561 tacgcgatct tcatcgtcaa caacgcgcag ggcacaatcc cgcccggcca gcaaacgtac
     1621 gagtccacca gcgccggcac ctcactaggc ccgatccagg gggtgaccga cgtgagctgg
     1681 accgacgagt gggcgcgctt tctgtacaac aacggcatca gcgtctacat cctggacgca
     1741 tactacgcgc agcagaatgt ctcgcactcc agtgtgctca agcgggcagc cgacatcgcc
     1801 ggaggccggt acatcaaggt cagcaaacag gaagatatcc agctggcgat cgaggacatt
     1861 cttgcggaaa tccgggccat caacacgacg ttcgcggccg ccagcctgcc gatcagtgcc
     1921 accaatcgct cccagaacct gaaccaggtg ttcatcggaa tgttccggcc cgcggacacg
     1981 aacaagccgc gctggatggg caacctcaag cagtaccagc tcggcacgag cgccagcggt
     2041 gtcgacttgg tcgacaggct cggcatgaat gcggtcaact cgcagaccgg cttcgtgagc
     2101 gagtgcgctg tcagcctctg gaccaccggc tccggcacct attggcagaa tgtcactgcc
     2161 aatcgactgg cgcgcagcgg ctgcgtgaat ttcccgacgg tggacggtgt ccttggcgcc
     2221 gaatggtcgg attcccccga tggggcaacg gtcgaaaagg gcggtgtggc tgaaatcctg
     2281 cgcaagggga acaatccacc ggctaccgat acgtcgccca cctggaccaa caaccgcacg
     2341 atcctgacct attcctcggc cactgccacg aagctgaccc cgctgacgac cgccgccacc
     2401 ggctggacgt ccacgcttca tgactgggtc accggtatcg acgaccccgc ggtcggctac
     2461 acggaattca tcgacacgag cgtcagcgcc cgcacccgcg catccatcca tggcgatgtg
     2521 attcattcca ggccgttgcc ggtcaactac gggggcacga ccggcgtgac cgtgtactac
     2581 ggaaccaacg acggcatgct gcgcgccgtc gacgccgcga cgggcaagga gcgttgggcc
     2641 tatgtggcgc ccgaacacta tgcgaattac cagcgcctgt acgacaactc gccggtcgtc
     2701 accttccaga atgccgaccc gacgtccagt gccacctcca agaagtactt cttcgacggg
     2761 tcgattggtg tttaccagaa ggcggacaac tcgcaggtgt ggatcttccc gtcgatgcgg
     2821 cggggcggcc gcatgctgta cggcttcgac gtgaccaacc cggcgtcgcc cattctcaag
     2881 tggcgcgtgg ggtgccccaa ccagaccgat aacaccggct gtacctccgc tgaattcgag
     2941 gcgatgggcc agacatggtc gttgccgagc gtggcttttc tcaagccgta tgatctcaat
     3001 gccgcaaaga tccccgtggt ggtggtcggc ggcggctatg acggctgtga ggatgaggat
     3061 gttcccatcc cgacttgcac cggtcgcaag ggcaaggcgg tctacgtcct gaatgccgac
     3121 acgggagccg tgatcaagaa gttcgtcccc acggaggacg cgggcagctt cgcggccgac
     3181 gtcgcgatgg ccgacgccaa cggcgacggt gtggtggacc atgcctatgc ggtcgacacc
     3241 aggggcaata tctaccggat cgatttctcg agtgactcga acgagccggc ggcatacggc
     3301 tcgtggacca tgcggcgcgt cgcttacacg tcgggggcgg gacgcaagtt cctgtacccg
     3361 ccgtcgctcc tcagggtggc gaacaagatg tatgtcgcgc ttgggtccgg tgatcgggag
     3421 cgcccgctgt tgggaaacta tccctgggat acaaacgttg tcaaccgctt ctatgtgctg
     3481 ctcgatgacc tgaccatacg ggacgcggcg gctcctgccg taccgttgga caccgaccca
     3541 ggcatgaagg atttcactac gctggcgtcc acctcctgtg acgaaagcgg ggtattgccg
     3601 acatcgggct tgaaaggctg gttcatgagc cttcccaacc gcggagaaca ggtggtgact
     3661 tccgctctta tcgccgccgg catggtcgct ttcaacacca atcgcgccgt tccctcggcg
     3721 gaggcggcgg cgtgtgtagc tcccttgggc gaggcgcggg gctactggct gaatctcgtg
     3781 aatgcgagcg gggcgatcgc cgcgggcaac aagacctgcg ggggcgacag gtcctcggtc
     3841 ttcgtgggcg gcgggctgac accgtcgccc accctggcca gggtcgtgat cggcaacagg
     3901 gtcaccacgg tcgcgatcgg tgccgccaac cgcgacggcg gcgtcagcgt gccgatcgac
     3961 ccacaggagg tgaaaccggt gatcagttcc aagcgcagga cgatctactg gaaatcgaac
     4021 gccgccgatt gacctcacga tttcatcgac gcggcccgca ggagcgggat gttaaaaagt
     4081 cacccgtttg ctctacatta gtggcatgaa gttctagcac gactcgccta ttgtccggtg
     4141 atggacacac cgaacaataa gcccgagtcg cgcagatccc gttcaaccca tcacggccgc
     4201 gcttcgcgcc ggccgttggg tttcaccctg gtcgagttga tctcggcggt cggcgtgatg
     4261 gccatcctgg cggctttggc catgccttcg atggcgcgca tcgtcgactc gacgcggttg
     4321 acagcgatat cgaacgactt tctctcttcc gtttacctgg cgcgaagcga ggccgtcaaa
     4381 cgcaaccggc cggtgggcct ttgcaagtcc gcgaccggag tcagctgcac gtcgaccggc
     4441 ggctgggagc agggatggat cgtgttccat gaccccaaca acaacggcag cgccgatgcg
     4501 ggggaagcgg tcgtccatca cacccaggcc ttgccgcagg gtttcaagct gtccggcaac
     4561 ttgaacgtgg cgaaatacat ctcgttcaca ccgttcgggc ggacccggat ggtcagcggc
     4621 gcctttcagg caggcaccct cacgctgtgc aaggcaaacg ggacggaaat tggagcgcgg
     4681 gagatcgtga tcaatcacgt cggccgtgca cgtgtgaaca agcgctcgat cagcgcctgc
     4741 gcttgagcca gcttgccctc agcgccgcac ttcgtccacc agcgtcttga actcgtcgat
     4801 gtcctcgaag ctgcggtaca cgctggcaaa gcggacatag gcgaccttgt ccagcttttt
     4861 cagctcgcgc atcaccagct cgccgatgcg cgccgaggga atctcgcgca cccccaggtt
     4921 cagcagcttc tcctcgatgc gctccacggc gccgtcgacc tgctcggtgc tcaccgggcg
     4981 cttgcgcagc gccaggttca tcgaggcctg cagcttggcg cgctcgtagt cgatgcgccg
     5041 tccgtccttc ttgacgacgg ccggaaagct cacatccggg cgctcgtacg tggtgaagcg
     5101 cttctcgcac cccaggcact ggcggcgcct gcgaatgaaa tcgccgtcct ccgccatgcg
     5161 cgtttcgacc acctgggtct cggcgtttcc gcaaaaaggg catttcatgg ccggcgcctg
     5221 cgcttatccg taaaccggga accggcgcgt cagcgcattg accttctcac gcacctgcgc
     5281 gatggtcgcc gcatcacgcg ggttgtccag cacgtcggca atgagattgg cggtcaggct
     5341 cgcttcttcc tccctgaagc cgcgggtggt catggccggc gtgccgatgc gcacgccgct
     5401 ggtcaccatc ggcttctccg ggtcgttcgg gatggcgttc ttgttgatgg tcatgtgggc
     5461 gtcgcccagc acggcctcgg cctccttgcc ggtgatgccc ttggcccgca ggtccaccag
     5521 catcacatgg ctttcggtgc ggccgctgac gatccgcagg ccgcgctcca ccagcgtctg
     5581 cgccaccacc tgggcgttcc tcgccacttg ctgctggtaa accttgaact cgggcgccag
     5641 cgcttccttg aaagccaccg ccttggcggc gatcacgtgc atcagcggac cgccctgcag
     5701 gcccgggaag atggcgctgt tgacggcctt ctcgtgctcg ggcttcatca ggatgaaccc
     5761 gccgcgcggg ccgcgcaggc tcttgtgggt ggtggaggtc acgacgtcgg cgtggggcac
     5821 cggattggga tagacgcccg cggcgatcag gccggcgtaa tgcgccatgt ccaccatgaa
     5881 aatcgccccc acgtcgcgcg ccaccttggc gaaccgctcg aagtcgatgc gcagggcata
     5941 ggcggaggcg ccggcgatga tcagcttggg cctggactcg tgcgccttgc gctccatcgc
     6001 gtcgtagtcg atctcttcct tttcatcgag gccgtaggag acgacgttga accacttgcc
     6061 gctcatatta agagccatcc catgcgtgag gtggccgcct tcggccaggc tcatgcccat
     6121 gatcgtgtcg ccgggcttca ggaaggccag gaacacggct tcgttggcct gcgcgcccga
     6181 gtgcgcctgc acgttggccg cgtcggcgcc gaacagctgc ttcacccgcg ccagcgccag
     6241 ctgttcggcc acgtcgacgt tctcgcagcc gccgtaatag cgcttgcccg ggtaaccttc
     6301 ggcgtacttg ttggtcagct gggttccctg cgcggccatc acggccggcg aggcgtagtt
     6361 ttcgctgg
//
LOCUS       DASWIY010000281         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2043949, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000281 DASWIY010000000
VERSION     DASWIY010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043949"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VGA59_10320"
     CDS             complement(<1..921)
                     /locus_tag="VGA59_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX9720115.1"
                     /translation="MDEIQSLMHGFTVVLTPTNLGLMFTGVILGVIIGVLPGLGGANG
                     VAILLPLTFSMAATPEGQTSAIILLSCIYWGALFGGAITSILFNIPGEPWSVATTFDG
                     YPLAQKGQAGTALTAAFTSSFVGALVAVILITFIAPVIASFALAFGPPEFFSVYLLTF
                     CAFVGMSKEPPYKTIAAMMTGFALAAVGTDPVTGTLRLTYGFTKLLTGFDFLVAVIGL
                     FGIGEIFLTLEEGLEFRGTRAKLNMRVVLETWKELPRYWLTSLRSAAVGCFMGIVPGG
                     ATPASFMAYGVARRFSKDGDKFGTGQVEGIV"
     gene            complement(926..1432)
                     /locus_tag="VGA59_10325"
     CDS             complement(926..1432)
                     /locus_tag="VGA59_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX9720116.1"
                     /translation="MSQQPAERAAFSLRSAEIGIALVFLLFAALVMFDSVRQGARWLE
                     EGPQSGYFPFYLGVIIALASTVTLVRALMMPAARNKAFVQVGQLKLVLSVLVPSAVYV
                     ALVGWIGIYVSAVLFVALFMRWLGKYPWWKVGTVSVGHSVVLFCIFEIWFLVPLPKGP
                     LERLLGLG"
     gene            complement(1495..2487)
                     /locus_tag="VGA59_10330"
     CDS             complement(1495..2487)
                     /locus_tag="VGA59_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9720117.1"
                     /translation="MKNNVLRVLAAAFAFGWLGAAQAWEPTRNIEFIIPAGTGGGADQ
                     MARAIQAIVAKHNLAKVAILPINKSGGAGAEGFLDVKNSPGEPHKIIITLSNLFTTPL
                     ATNTPFNWRDLTPVQMLALDNFVLWVKTDDPYKTPKDYIAAAKAAGPGKFRMAGTGAK
                     QEDQIITVALEVSTGAKFTYIPFKGGGAVATQLVGGHVNSTVNNPIEAVAHWRGGKLK
                     PLCVFELNRLPYKAKVTSTMSWADIPTCKETGLDIDYLMLRGVFMPAGVSKEHVDYYL
                     GLFTKVFATKEWKDLMERGAFNQTKLKGQEYAAWVAKEEARHVSLMKAAGFIAK"
BASE COUNT          498 a          909 c          891 g          452 t
ORIGIN      
        1 gacgatgccc tccacctgcc cggtgccgaa cttgtcgccg tccttggaaa agcgccgcgc
       61 cacgccgtag gccatgaacg aggccggcgt cgcgccgccc ggcacgatgc ccatgaagca
      121 gcccacggca gcggagcgca gcgaggtgag ccagtagcgc ggcagctcct tccacgtctc
      181 gagcaccacg cgcatgttga gctttgcgcg cgtgcccctg aattccagcc cttcctccag
      241 cgtgaggaag atctcgccga tgccgaacag gccgatcacc gccaccagga agtcgaagcc
      301 ggtgagcagc ttggtgaagc cgtaggtgag gcgcagcgtc ccggtcaccg ggtcggtgcc
      361 gacggcggcg agtgcgaaac ccgtcatcat cgcggcgatg gtcttgtacg gcggctcctt
      421 gctcatgccg acgaacgcgc agaacgtgag caggtagacc gagaagaact cgggtgggcc
      481 gaaggccagc gcaaagctcg cgatcaccgg cgcgatgaag gtgatcagaa tcactgcgac
      541 cagcgcgccg acgaaggacg acgtgaaggc ggcagtgagc gcggtgccgg cctgtccctt
      601 ttgcgccagc ggatagccgt cgaaggtggt ggccaccgac cagggctcgc ccgggatgtt
      661 gaagaggatg ctggtgatgg ctccgccgaa cagtgccccc cagtagatgc aggacagcag
      721 gatgatggcc gaggtctgcc cctccggcgt cgccgccata ctgaaggtga ggggcagcag
      781 gatggcgacg ccgttcgccc cgcccaggcc cggcagcacg ccgatgatca ccccgaggat
      841 cacgcctgtg aacatcagcc ccaggttcgt cggcgtcagc acgacggtga agccgtgcat
      901 cagggactgt atttcgtcca tcgcgctagc ccagtcccag caagcgctcg agcggcccct
      961 tgggcagcgg cacgagaaac cagatctcga agatgcagaa cagcaccacg ctgtggccga
     1021 ccgagacggt ccccactttc caccagggat acttgcccag ccagcgcatg aacagcgcga
     1081 cgaacaggac cgcggacacg tagatgccga tccagccgac cagcgcgacg tacaccgcgg
     1141 aggggaccag cacgctcagc acgagcttca actgccccac ctgcacgaac gccttgttcc
     1201 gggcggcggg catcatcagc gcccgcacca gggtcaccgt cgacgcgagc gcgatgatga
     1261 cgcccagata gaaggggaag tagcccgatt gcgggccctc ctccaaccag cgcgcaccct
     1321 gccggacgct gtcgaacatc accagcgcgg cgaacagcag gaaaaccagc gcgatgccga
     1381 tctccgcact tcgcaggctg aatgccgcgc gctctgccgg ttgctgggac atgcccttcc
     1441 cttcaggtca aaaaaaggcc cggcacgtgc cgggcctgtt tgcgccgtac cgcgttactt
     1501 ggcgatgaag cccgccgcct tcatcagcga gacgtggcgc gcctcttcct tcgccaccca
     1561 ggccgcgtac tcctggccct tcagcttggt ctggttgaaa gcgccgcgct ccatcaggtc
     1621 cttccattcc ttcgtcgcga acaccttggt gaacaacccg aggtagtagt cgacgtgctc
     1681 cttggagacc cccgcgggca tgaagacgcc gcgcagcatc aggtagtcga tgtcgagccc
     1741 cgtctccttg caggtcggga tgtcggccca ggacatggtg ctggtgacct tcgccttgta
     1801 gggcaggcgg ttcagctcga acacgcacag gggtttcagc ttgccaccgc gccagtgcgc
     1861 cacggcctcg atggggttgt tgaccgtcga gttgacgtgg ccgccgacca gctgcgtggc
     1921 caccgcgccg ccgcccttga acgggatgta ggtgaacttc gcgcccgtgg acacctcgag
     1981 cgccaccgtg atgatctggt cctcctgctt ggcgccggtg ccggccatgc ggaacttgcc
     2041 cgggcccgcc gccttcgccg ccgcgatgta gtccttgggc gtcttgtagg gatcgtcggt
     2101 cttcacccac agcacgaaat tgtccagcgc cagcatctgc accggggtca ggtcgcgcca
     2161 attgaacggc gtgttggtgg cgaggggcgt ggtgaacagg ttcgacagcg tgatgatgat
     2221 cttgtgcggc tcgcccgggg agttcttcac gtcgaggaag ccctcggcac cggcgccgcc
     2281 cgacttgttg atcggcagga tcgccacctt ggcgaggttg tgcttggcga cgatggcctg
     2341 gatggctcgc gccatctggt cggcgccgcc gcccgtgcct gccgggatga tgaattcgat
     2401 gttgcgggtg ggttcccagg cctgcgccgc cccgagccag ccgaaggcga atgccgctgc
     2461 gagaacgcgc aaaacgttgt tcttcatagt ccctcctttg tcggttgtag ctgtcgccgc
     2521 ggcgcaccat tcttcggact ggcttgggca gtgctgcgct ggccaattct gagttctacc
     2581 ccaaacgttt cacattgacc agcgtcaaga aaacggcgcg ccggcgcatt tcacccttgg
     2641 gcaaggacat acacggcgta cagcgcaccg atgtagacga cgggaatcgc cggggccagg
     2701 gcgcgcaacg gcgcgaatcg gcgcgactgg caggatttga acacgaggta
//
LOCUS       DASWIY010000282        11220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1672590, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000282 DASWIY010000000
VERSION     DASWIY010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11220
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672590"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VGA59_10335"
     CDS             <1..892
                     /locus_tag="VGA59_10335"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEX9720118.1"
                     /translation="RRTTVNLKECIRAAKDRVVFINTGFLDRTGDEIHTSMELGPMIR
                     KGDMKEAAWIGAYEDWNVDMGLECGLKDRAQIGKGMWAMPDLMAAMLETKIGHPKAGA
                     NTAWVPSPTAATLHALHYHQVNVAEVQRELEKIDADAQREALLAGLLQVPVAAEAKWS
                     AAERQQEIDNNAQGILGYVVRWIDQGVGCSKVPDIHNVALMEDRATLRISSQHIANWL
                     LHGVVSEEQVKETFRRMAAVVDGQNAGDPFYKPMAGNFETSAAFKAACDLVFKGLVQP
                     SGYTEPLLHAWRLKVKAGLA"
     gene            1088..3532
                     /locus_tag="VGA59_10340"
     CDS             1088..3532
                     /locus_tag="VGA59_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEX9720119.1"
                     /translation="MAPQSSALQQCFDEVADSAPMALERCLDHVVTVLQEAECSSARA
                     DERMELGDAWRELLAHRAAWCKRYPDELRAAFKTAAAPKTESAPDAGAGRDRRMPLEL
                     ALVDDADIMQEIESSRLVRYVTPLVEQPVSELDALVSSAMGLGSVRPELNPVRPEVFA
                     QTLRALIGHSQVKPATGTLWMKYMAEPLGAELQHLYGRLVTQFKNANVQPADYRSVPA
                     SPAPGPRAAAASRVAPAGAATAPQAHMAAPPKPDSCFDSRQISHELLREFLEGSGSEA
                     SQSLPASYYTEAEEELAQLRSLTGGVNVAAPPVVPAGYRELPAVERPQLPVGVQSTLD
                     PEVWGDYANSHERSLVHAQLRKDAMRVAQVLGLELVRKVVNQIARDPKLLAPVREAIV
                     ALEPSLLRLAMVDPRFFSEEQHPGRLLMERVAQRSFRYNDEFAADFAVFFQGVTRCFN
                     GLNGAAIPDALPFERALAQLEAVWIELDRKDEEQRSQAVKAMRFAELRQQEADQIAWE
                     LSSRPDLENVPSVVQDFLFGRWALVLAHARLTDTAHQIDPHGYRSLISDLLWSVKREV
                     TLKQPAQLFARVPGLVASLRAGLTSIGEEPQENEAFFQALMQLHHPVLKLRRAKSRRD
                     ARESGMVPLSAVDRAAAPEAAATQATTEPADGQPWMSPHELDAFGFEETLPTDWAQLE
                     AESTSSSPAPLMPQAERNPAPADRQLPTRAAPSAPDVESVMSTLREGDWFDLYSKRRW
                     LRAQLIWASSKATLFMFVSHGGRPHSMTKRVCERLIRDRYLRPVRTHGVVSRALDTLD
                     QEPAADAERRPLSPAP"
     gene            3567..3791
                     /locus_tag="VGA59_10345"
     CDS             3567..3791
                     /locus_tag="VGA59_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HEX9720120.1"
                     /translation="MNTVAATATYCPLCGQPNQCAMEIERATGVEQPPCWCTQQKFDA
                     DLMARVPEDGRGKVCICPACSRRTGVHSVD"
     gene            complement(3793..5739)
                     /locus_tag="VGA59_10350"
     CDS             complement(3793..5739)
                     /locus_tag="VGA59_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HEX9720121.1"
                     /translation="MHTHLDEHAPLGRRRNKVVSAAEAVRLIHDGDTVATGGFVGIGF
                     AEEVAMALEARFLATQAETGTGSPQGLTLVYAAGQGDGKERGLNHLGHAGLVRRVVGG
                     HWGLVPALQRLAVSNAIEAYNLPQGVLTHLFRDIAAGKPGTLTHVGLDTFVDPRHGGG
                     RVNARTTEEIVRLMEIDGREYLFYKAFPISVGIIRATTADPDGNLTMEREALTLEVLA
                     IAMAARNSGGIVIAQVERVAERGTLHPRQVKVPGILVDAVVVATDPAHHMQTFVEQYN
                     PAYSGEIRLPVDHLVAMPLGIRKIIARRAALELKTNSIVNLGIGMPEGVAAVAAEERV
                     IDLVTLTAEPGVIGGIPASGLSFGAAVNAQAVIDQPNQFDFYDGGGLDLAVLGLAQAD
                     AEGNLNVSRFGPRLAGAGGFINISQNAKTVVFVGTFTAGDLSVELVGGRIRIASEGTM
                     RKFVRQVEQRTFSGREARRRGQRVLYVTERCVLRLADAGGLELIEIAPGIDLERDVLG
                     QMDFMPAISPDLALMDRALFADAPMDLRVRLLAKPLSERYELDRENRLLFINFEGLNI
                     DNRSAIAEIEQHVTQLLGPVVTSPDERLAVVVNYDNFSILPGLVDEYSAMVMRLTDRF
                     YSHVTRYGTHGFLKARLEGRIAGS"
     gene            5834..7288
                     /locus_tag="VGA59_10355"
     CDS             5834..7288
                     /locus_tag="VGA59_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX9720122.1"
                     /translation="MTAADSLSALRERYGPRYRWLLLLSVMIGTMASIMSSTIMNVAI
                     PDLSQHFTLGQERAQWVSSGFMVAMTVSMLTTPWLLARYGYRRTYVGTMLLLMAGGIV
                     GGFSNHFTVVLAARVAEGLAAGVVQPIPAIIILRAFEPNEQGRASGVFGMGVVLAPAL
                     GPSIGGVLVDLFGWRSIFFMVVPFCLASIWLALKFVPVSAPGGGAAQPGNRLDWRGLV
                     LGSAGTLCLLNGLVQLHGGGASAGVLLLGAAAALVAFLAWQRRLMAGGGEPLMNLALF
                     GSRSFAMGSIVAFIYGTAMFGSIYLLPVYMQLALGLSPSYVGTILLPSGLVLAVTIPL
                     AGRLADHQPMHLLVSIGLALLAASFVLMVTISLGTGIWLLVAWTTLGRIGLGFILPSL
                     HIGSMRALDKSLIPQGSSAINFLRMLGGAAGVSLCGIVLEWRIAAHGDSLANPVGSAA
                     RLAAFNEVFLMLAAVCALALAAAWRLRERSNGTG"
     gene            7275..8234
                     /locus_tag="VGA59_10360"
     CDS             7275..8234
                     /locus_tag="VGA59_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720123.1"
                     /translation="MGQAEARAPVMLWVGELLVPFHHGQAHPNAIDRQEVFVDEARRQ
                     GLLDRVAIGLPRAATREELLRFHDEAHVNWVRHCSLEGTGQLGDEDTLAYSGVYEDAA
                     ARVGTALEAAARLMDGTARRCFQPVGGTHHARRHRGGGFGPFSDIGVVIESLRAHYGV
                     RRIAYADIDAHHGDGVYDAYQHDPHLAIADIHQDGATLFPGTGSAQDTGHGPAAGSKL
                     NLPLAPGATDGQFQAAWARAQAHLEAREPEFVILQCGADSLAGDPLADLCFTAACHEH
                     AARQLCALADRHARGRLLVLGGGGYAAANVAAAWTGVLRALLA"
     gene            8284..9048
                     /locus_tag="VGA59_10365"
     CDS             8284..9048
                     /locus_tag="VGA59_10365"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX9720124.1"
                     /translation="MCQLLGMNCNTPTDVTFSFTGFAQRGGRTDQHVDGWGIAFFEGA
                     GLRHFVDHQPACESPVAELIRRYPIKSRNVIAHIRKATQGAVALENCHPFVRELWGRY
                     WVFAHNGNLEDFHPRLHASFRPVGATDSERAFCWLMQEMSKSHAGVPSVQELTLTLRE
                     LTPRIARHGPFNFMLSNGQALWAHCSTNLFFVERKHPFAHAHLADEDLSIDFARNTSP
                     HDRVAVVVTAPLTTNETWTAFEPGDLRVFVDGQATG"
     gene            complement(9050..9997)
                     /gene="gshB"
                     /locus_tag="VGA59_10370"
     CDS             complement(9050..9997)
                     /gene="gshB"
                     /locus_tag="VGA59_10370"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX9720125.1"
                     /translation="MNILFVADPLEVFQTYKDTTFSMMREAQRRGHRLAACEPRHLLW
                     RSGDLVKAHVRDITLTGALNDWYEECGRGIKVLKDFDVVLMRKDPPFDSEYFYATHLL
                     EQAGREGARVFNRPASLRDHPEKLAIMEFAQFASPTLVTRNAEDVKRFHAEHQEVILK
                     PLDGMGGMGIFRVGADGLNLGSIIETLNHHGTTTIMVQRFVPEIAQGDKRILVIGGQP
                     VPYSLARIPQGSEIRGNLAAGGKGVAQPLSARDFEIAQALGPVLAARGLLLVGLDVIG
                     DYLTEINVTSPTCFQEITQQAGFDVPAMFIDALEAAVPR"
     gene            complement(10082..>11220)
                     /locus_tag="VGA59_10375"
     CDS             complement(10082..>11220)
                     /locus_tag="VGA59_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX9720126.1"
                     /translation="LAWFAIVMPALTLNYFGQGALLLKNPEAVKNPFYMMAPDWALLP
                     MVALATAATVIASQAMITGAFSVTKQVIQLGYLPRLQIRHTSVRDTGQIYIPFVNWGL
                     FVAVVLAVVLFRSSSNLAAAYGITVTLDMLITTTLTFFVIRYSWNYPLALCVAATGWF
                     FMVDLAFFSSNLLKIAQGGWFPLVIGGAVFMLMITWKRGRELLGEKLKADAIDLKSFL
                     EAVFVSPPMRVEGTAVFLTAEPGTVPNAMLHNLKHNKVLHENNLFVTVQSHEVPWIGI
                     SKRVEIEPLGHSCWQVVLHYGFKNDPDVPRALEQIRARGCEIEKMKTSYFLSRDTVVP
                     TIGRGMAPWREKLFAQMHHNASAAADFLNLPNNSVVELGSKIEI"
BASE COUNT         1831 a         3716 c         3767 g         1906 t
ORIGIN      
        1 acgccgtacc acggtgaacc tgaaagagtg catccgcgcc gccaaggacc gcgtcgtgtt
       61 catcaacacc ggtttcctcg accgcaccgg cgacgagatc cacacctcga tggaactggg
      121 accgatgatc cgcaagggcg acatgaagga ggccgcatgg atcggcgcct atgaggactg
      181 gaacgtcgac atgggcctcg aatgcggcct caaggatcgc gcccagatcg gcaagggcat
      241 gtgggccatg ccggacctga tggccgccat gctggaaacc aagatcggcc acccgaaggc
      301 cggcgccaac acggcctggg taccgtcgcc gacggccgcc acgctgcatg cattgcacta
      361 ccaccaggtc aatgtcgcgg aagtgcagag ggagctggag aaaatcgacg ccgacgcgca
      421 gcgcgaggcc ctgctggcgg gcctgctgca ggtcccggtc gccgccgaag ccaaatggag
      481 cgccgccgaa cgccagcagg aaatcgacaa caacgcccag ggcatcctgg gctacgtcgt
      541 gcgctggata gaccagggcg tgggctgctc caaagtgccc gacatccaca acgtggcatt
      601 gatggaagac cgcgccacgc tgcgcatcag cagccagcac atcgccaact ggctattgca
      661 cggcgtggtg agcgaagagc aggtgaaaga aacgttccgg cgcatggccg ccgtggtgga
      721 cggccagaac gccggggatc cgttctacaa gcccatggcc ggcaacttcg aaacgtcggc
      781 ggctttcaag gctgcatgcg acctggtgtt caagggcctg gtgcagccca gcggctatac
      841 cgagccgctg ttgcatgcgt ggcggctgaa ggtgaaggca gggttggcgt agcgtcgtcg
      901 gggccgaacg gtgtctccag cccgacgagc ggcacgtccg gcgccggaat tggtaacgag
      961 ttattgctga ggttgcagaa cctctggggg ttgctacaaa ctcgctaaac ctgctcggca
     1021 ataagggctc ggtcacctcc atgccgattc agctcctgcc cagtttcctg ccgaggtttg
     1081 gataccgatg gctccgcaat cgtctgcgct tcagcagtgt ttcgacgagg tcgccgactc
     1141 ggcgcccatg gcgctggagc gttgcctcga ccatgtcgtc acggtgctgc aggaggcgga
     1201 atgctccagc gcgcgagcgg atgaacgaat ggagctggga gacgcatggc gtgagttgct
     1261 ggcgcaccgg gccgcctggt gcaaacgcta tccggacgag ttgcgcgccg ccttcaagac
     1321 cgcggcggcg cccaagaccg agtcggcccc ggatgcgggc gccggccgcg accggcgaat
     1381 gccgctggag ttggcgctcg tggacgacgc ggacatcatg caggagatcg agtcctcgcg
     1441 gctggtgcga tatgtgacgc cgctggtgga gcagcccgtg tcggaactcg atgcactggt
     1501 cagttcggcc atgggcctgg gcagtgtgcg gcccgagttg aacccggttc gacccgaggt
     1561 ttttgcccag accctgcggg cactgatagg tcatagccag gtgaagccgg ccaccggaac
     1621 gctgtggatg aaatacatgg ccgaaccgtt gggtgcggaa ctgcagcact tgtatggcag
     1681 gctggtcacc caattcaaga atgccaacgt gcagccggcg gactaccgct cggtacccgc
     1741 ttcgccggcg ccggggcccc gcgcggcggc cgcgagccgg gtcgcccccg cgggtgcggc
     1801 aacagcgccc caggcccaca tggcggcacc cccgaaaccg gactcctgtt tcgacagcag
     1861 gcagatcagt catgagctgc tgcgggaatt tctggaaggc agcggcagcg aggcgagcca
     1921 gtcattgccc gcctcgtact acaccgaggc ggaagaggag ctggcccaac tgcgcagctt
     1981 gacgggtggc gtgaacgtcg ccgcgccgcc agtggtgccc gccggctatc gcgagttgcc
     2041 cgcggtcgag cggccgcagc tgcccgtagg cgtgcaaagc accctcgatc ccgaggtttg
     2101 gggagactat gccaattcgc atgagcgctc actggtgcac gcgcagctgc gcaaggacgc
     2161 aatgcgcgtg gcccaggtgc tcggcctcga actggtgcgc aaggtggtca accagatcgc
     2221 ccgggacccg aagctgctgg cgccggtacg cgaggccatc gtcgcgctcg aaccttcgct
     2281 gttgcggctg gccatggtgg atccgcgctt cttcagcgag gagcaacatc cgggccgctt
     2341 gctgatggaa cgggttgccc agcgcagctt caggtacaac gacgaattcg ccgccgactt
     2401 cgcggttttc ttccagggcg tcacccgttg cttcaatgga ttgaacggcg cggcgattcc
     2461 cgacgcgctg ccgttcgagc gggcgctggc ccaactcgag gccgtttgga tcgagctgga
     2521 ccgcaaagac gaggagcagc gcagccaagc cgtcaaggcg atgcgctttg ccgaattgcg
     2581 gcaacaggaa gcggatcaga tcgcctggga actgagttcg cgccccgatc tcgagaacgt
     2641 gccttcggtg gtgcaggatt tcctgttcgg ccgttgggcg ctggtcctgg cgcatgcgcg
     2701 cttgacggac acagcccacc agatcgaccc gcacggatat cggtcgctga tctccgacct
     2761 gttgtggagt gtcaagcgcg aggtgactct caagcaaccg gcccagcttt tcgcgcgcgt
     2821 gccggggctg gtggcatcgc tgcgggcggg gctgacctcg attggagagg aaccgcaaga
     2881 gaacgaagct ttcttccagg ccttgatgca actgcaccat cctgtcctga agctgcggcg
     2941 cgccaagtcg cgccgcgacg cgcgcgagtc gggcatggtg ccgttgtccg ccgtcgatcg
     3001 cgctgcggcg ccggaagctg cagcgacgca agccacgacc gaaccggcag atggccagcc
     3061 atggatgagc ccccacgaac tcgacgcctt cggattcgag gaaacgctgc cgaccgattg
     3121 ggcgcagctg gaggccgaat ccaccagttc ctcgccggcg ccgctgatgc cacaggccga
     3181 aaggaacccg gcccctgccg atcgacagct tcccacgcgc gcggcgccca gcgcgcccga
     3241 tgtcgagagc gtgatgtcca cgctgcgcga gggcgactgg ttcgacctct actcgaagcg
     3301 gcgctggctg cgcgcccagc tgatctgggc cagcagcaag gccaccttgt tcatgttcgt
     3361 gagtcacggc gggcggcccc actcgatgac caaacgggtt tgcgaacgcc tgatccggga
     3421 ccgctacctg cgcccggtac gtacgcacgg cgtcgtgtcc cgcgccctgg acacgctcga
     3481 tcaggaaccc gccgccgacg cggaacggcg ccccctcagt ccagcgcctt gagccactcc
     3541 agcacggtga agtgtatgct gcacgcgtga ataccgtcgc cgccaccgcc acgtactgtc
     3601 ccttgtgcgg gcagcccaat caatgcgcga tggaaatcga gcgcgccacc ggcgtggagc
     3661 agccgccctg ctggtgcacg caacagaaat tcgatgccga cctgatggca cgtgtgcctg
     3721 aagacgggcg cggcaaggtt tgcatctgcc cggcctgctc gcgccgcacg ggagtccact
     3781 cagtggacta ggctaggaac ccgcaatccg gccttccagc ctggccttca ggaagccgtg
     3841 ggtcccatac cgcgtgacgt ggctgtagaa gcggtcggtc aggcgcatga ccatggcgga
     3901 atactcgtcg accaggccgg gcaggatgct gaagttgtcg tagttgacta ccacggccaa
     3961 tcgctcgtcc gggcttgtca ccaccggtcc gagcaattgc gtcacatgct gctcgatctc
     4021 ggcgatcgcg ctcctgttgt cgatgttcag cccttcgaaa ttgatgaaca gcaaccggtt
     4081 ctcgcggtcc agttcgtacc gctcggacag cggcttggcc agcagccgga cgcgcagatc
     4141 catgggagcg tcggcgaaca gcgcgcggtc cattaacgcg aggtcgggag agatcgccgg
     4201 catgaagtcc atctgcccca gcacatcgcg ttccaggtcg atgccgggcg cgatctcgat
     4261 cagctccagt ccgcccgcgt cggcgagacg aagcacgcag cgctccgtga cgtacagcac
     4321 gcgctggcct cggcgcctgg cctcccggcc gctgaacgtg cgttgctcca cctgccgcac
     4381 gaacttgcgc atcgtgcctt cactcgcgat gcggatcctt ccgcccacca gctcgaccga
     4441 tagatcgccg gccgtgaagg tgccgacgaa caccacggtc ttggcgttct ggctgatgtt
     4501 gatgaagccg ccggcgcccg ccaggcgcgg gccgaagcgg ctgacgttga ggttgccctc
     4561 ggcgtccgcc tgtgccaggc ccagcaccgc caggtcgagc ccgccgccgt catagaagtc
     4621 gaactggttc ggctggtcga tcaccgcctg ggcgttgacg gcggcaccga agctcaggcc
     4681 gctggcggga attcccccga tcacgccggg ttcggccgtg agcgtgacca ggtcgatgac
     4741 cctttcctcg gccgcgaccg cggccacgcc ttcgggcatg ccgatgccca ggttcacgat
     4801 gctgttggtc ttgagttcca gcgcggcgcg cctcgcgatg atcttgcgga tccccaaagg
     4861 catggccacg aggtggtcca cgggcagacg gatctcaccg gaataggcgg ggttgtactg
     4921 ctccacgaag gtctgcatgt ggtgggccgg atcggtggcg acgaccaccg catcgaccag
     4981 gatgcccggc accttgacct gccgcgggtg cagggtgccc cgctcggcga cgcgttcgac
     5041 ctgcgcgatg acgatgccgc ccgagttgcg cgcggccatc gcgatggcca gcacctcgag
     5101 cgtcagggcc tcgcgttcca tggtcaggtt gccgtcgggg tcggcggtgg tggcgcgaat
     5161 gatgcccacc gaaatcggaa aggccttgta aaacaggtac tcccggccat cgatctccat
     5221 caggcgcacg atctcctcgg tggtgcgcgc gttgacgcgg ccgccgccgt ggcgcggatc
     5281 gacgaaggtg tccagcccga cgtgggtcaa tgtcccgggc ttgccggccg cgatgtcccg
     5341 gaacaggtgg gtcagcacgc cctgcggcag gttgtacgcc tcgatggcgt tggatacggc
     5401 gagcctttgc agcgccggga ccagccccca gtggccgccc accacccgcc gaaccagccc
     5461 ggcatgcccc aggtggttca gaccacgctc cttgccgtcg ccttgtcccg ccgcgtatac
     5521 cagcgtcagg ccttggggcg atccggtgcc cgtttcggcc tgggtggcca ggaagcgcgc
     5581 ttccagcgcc atggcgactt cctcggcgaa gccgatgccg acaaagccgc cggtggccac
     5641 cgtatcgccg tcgtggatca ggcggacggc ctcggccgcg ctgaccacct tgttgcgccg
     5701 ccgtcccagc ggcgcgtgct cgtccaagtg cgtatgcaag gcgattcctc gtgaagtgct
     5761 cctatcatcg ccaggaacgg cgcctcgcgc cttcagggaa acccatgcca tcgacttctt
     5821 tcgcggagcc cgcttgacgg cagccgacag cctgtccgcg ctgcgggagc gctacggccc
     5881 gcgctaccgc tggctgctgt tgctgtcggt gatgatcggc acgatggctt ccatcatgtc
     5941 ctccaccatc atgaacgtgg cgatcccgga cctgagccag catttcacgc tgggccagga
     6001 gcgcgcgcaa tgggtcagtt ccggcttcat ggtggcgatg acggtttcga tgctgaccac
     6061 gccctggctg ctggcgcgct acggttaccg ccgcacttat gtcggcacca tgctgttgct
     6121 gatggccggc ggcatcgtcg gcggtttctc gaatcacttc accgtggtgc tggccgcgcg
     6181 agtggccgaa gggctggccg cgggcgtcgt gcaacccatt ccggcgatca tcatcctgcg
     6241 cgccttcgag ccgaacgagc agggccgcgc aagcggcgtc ttcggcatgg gcgtcgtgct
     6301 ggcgccggcc cttgggccca gcatcggcgg cgtgctggtg gacctgttcg gctggcgttc
     6361 catctttttc atggtcgtgc cgttctgcct ggcctcgatc tggctggcgt tgaaattcgt
     6421 tccggtgagc gcgccgggcg ggggtgcggc ccagcccggc aacaggcttg actggcgcgg
     6481 cctggtgctg ggcagcgccg gcaccctgtg ccttctcaac gggctggtgc aactgcacgg
     6541 cggcggcgcc tcggccggcg tgctgctgct gggtgccgcg gccgcgctgg tcgccttcct
     6601 ggcatggcag cgacggctca tggccggagg tggcgagccg ctgatgaacc tggcgctgtt
     6661 cggcagccgc agcttcgcga tgggcagcat cgtggccttc atctacggca cggcgatgtt
     6721 cggctcgatc tacctgctgc cggtctacat gcagcttgcc ctggggcttt cgccatcgta
     6781 tgtgggcacc atcctgttgc cctcggggct ggtgctcgcc gtgacgatcc cattggcggg
     6841 tcggctggcc gaccatcagc ccatgcacct gctggtgagc attggcctgg cgctgctggc
     6901 ggcatcgttc gtgctgatgg tcacgatcag cctcggcacc ggcatctggc tgcttgtcgc
     6961 ctggaccacc ctggggcgta tcgggctggg attcatcctg ccgtcgctgc acatcggctc
     7021 gatgcgggcc ctcgacaaga gcctgatccc gcaaggttcg agcgcgatca atttcctgcg
     7081 aatgctcggc ggtgccgccg gcgtgagcct gtgcgggatc gtgctggaat ggcgaattgc
     7141 cgcgcatggc gattcgctgg cgaatccggt cggcagcgcc gcccggctgg ccgcgttcaa
     7201 tgaggtattc ctgatgcttg cggcagtctg cgcactggcg ctggcggccg cgtggcggct
     7261 gcgggaaaga agcaatggga caggctgaag cgcgggcccc cgtcatgctc tgggtgggcg
     7321 aactgctggt gccctttcac catggccagg cgcatcccaa tgccatcgac cgccaggaag
     7381 tgttcgtgga cgaggccagg cgccaagggc tgctcgaccg ggtcgccatc ggcctgcccc
     7441 gtgcggccac ccgggaagag ttgcttcggt ttcacgacga ggcgcacgtg aactgggtgc
     7501 ggcattgctc gctcgaaggc acgggtcagc tgggcgacga ggacacattg gcctacagcg
     7561 gcgtgtacga ggacgccgcc gcccgcgtcg gtaccgcgct ggaggccgcg gcgcggctca
     7621 tggacggcac cgcgcggcgc tgcttccagc cggttggcgg cactcatcat gcgcgccggc
     7681 accgcggggg cggcttcggg ccgttcagcg acatcggcgt ggtgatcgaa tctcttcgcg
     7741 cccactacgg cgtgcggcgc atcgcctatg cggatatcga tgcccatcac ggcgacggcg
     7801 tgtacgacgc ctaccagcac gacccgcacc tggccatcgc cgatatccat caggacggcg
     7861 ccaccctctt tcccgggacc ggcagcgcac aagacacagg ccacgggccg gccgccggca
     7921 gcaagctcaa cctgccgctt gcgcccggcg ccaccgacgg gcagttccag gcggcctggg
     7981 cgcgcgcgca ggcgcacctg gaggcccgcg agccggaatt cgtcatcctg cagtgcggcg
     8041 ccgacagcct ggcgggcgac ccgctggcgg acctgtgctt cacggcggct tgtcatgagc
     8101 atgcggcacg gcagctgtgc gccctggccg accgtcacgc acgcggccgg ctgctggttc
     8161 tgggcggcgg cggctacgcg gccgcgaatg tcgccgcggc ctggaccgga gtgttgcgcg
     8221 ccctgcttgc ctgatcccgg gcgcaatgcg ccacaagccc agggcctggc caataatgga
     8281 gccatgtgcc agctgctcgg gatgaactgc aacacgccca ccgacgtgac tttcagtttc
     8341 acgggtttcg cccagcgcgg cggccgcacc gaccagcatg tcgacggctg gggcatcgcc
     8401 ttcttcgagg gcgcgggcct gcggcatttc gtcgaccacc agccggcttg cgaatcgccc
     8461 gtggccgagc tgatccgccg ttaccccatc aagagccgca acgtcatcgc ccacatccgc
     8521 aaggccacgc agggggcggt ggcgctggag aattgccatc cctttgtgcg cgagctgtgg
     8581 ggccgctatt gggtgttcgc gcacaacggc aacctggagg acttccatcc ccgcctgcac
     8641 gccagctttc gtccggtggg cgcgacggac agcgagcgcg ctttctgctg gctgatgcag
     8701 gaaatgtcca agtcgcatgc gggcgtgccc agcgtgcagg aactcaccct gacgctgcgc
     8761 gagctgacgc ccaggatcgc gcgccacggc ccgttcaact tcatgttgtc caatggccag
     8821 gccctgtggg cccattgctc caccaacctg tttttcgtgg agcgcaagca tccgttcgcg
     8881 catgcccacc tggccgacga ggacctgagc atcgacttcg cccgcaacac ctcgccgcac
     8941 gaccgggtcg ccgtggtcgt cacggcgccg ctcacgacca acgagacctg gaccgctttc
     9001 gagcccggcg acctcagggt gttcgtggac gggcaggcca ccggctaggc tagcgtggga
     9061 cggccgcctc cagcgcgtcg atgaacatcg cgggcacgtc gaaaccggcc tgctgcgtga
     9121 tctcctggaa gcaggtcggg ctggtcacgt tgatctcggt gagatagtcg ccgatcacat
     9181 cgagtcccac cagcagcagg ccgcgcgccg ccagaacggg gcccagcgcc tgcgcgatct
     9241 cgaagtcgcg cgccgacagc ggctgcgcca cgcccttgcc gccggcggcc aggttgccgc
     9301 ggatttcgct gccttgggga atgcgcgcca ggctgtaagg caccggctgg ccgccgatga
     9361 ccaggatccg cttgtcgcct tgcgcgatct ccggcacgaa gcgctgcacc atgatcgtgg
     9421 tcgtgccgtg gtggttgagc gtctcgatga tggagcccag gttgagccca tcggcaccaa
     9481 cgcggaagat tcccatgccg cccatgccgt ccagcggctt gaggatgacc tcctggtgct
     9541 cggcgtgaaa gcgcttcacg tcctcggcgt tgcgggtgac cagcgtcggg ctggcgaact
     9601 gcgcgaactc catgatcgcc agcttctccg gatggtcgcg cagcgatgcc ggccggttga
     9661 acacccgcgc cccctcgcgc ccggcctgct cgagcaggtg cgtcgcgtag aagtactcgc
     9721 tgtcgaaggg cgggtccttg cgcatgagca ccacgtcgaa gtccttcagc accttgattc
     9781 cccgcccgca ctcctcgtac cagtcgttca gtgctcccgt cagtgtgatg tcacggacat
     9841 gcgccttgac caaatccccc gagcgccaca aaaggtgccg cggctcgcag gcagccaggc
     9901 gatggccgcg ccgctgcgcc tcgcgcatca tggagaaagt cgtgtccttg taggtctgga
     9961 acacttccag gggatcggcg acgaagagga tgttcatggg aggggttgcc tgggtgcgac
    10021 tgtactgttg cacaaaaaat cccggctcgc tggcgccgcc ccgattgcgg cgtgccgccg
    10081 gtcagatctc gattttcgaa cccagctcca cgaccgaatt gttcggcagg ttcaggaaat
    10141 ccgcggccgc gctggcgttg tgatgcatct gcgcgaacag tttttcgcgc cacggcgcca
    10201 tgccccggcc aatggtgggg acgacggtat cgcgcgacag gaaatagctg gttttcatct
    10261 tctcgatctc gcagccgcgg gcgcgaatct gctcgagcgc gcgcggcacg tccggatcgt
    10321 tcttgaagcc gtagtgcagc accacctgcc agcagctgtg gcccagcggc tcgatctcga
    10381 ctcgcttgct gatgccgatc cacggcactt catggctttg cacggtgacg aacaggttgt
    10441 tctcgtgcag caccttgttg tgcttgaggt tgtgcagcat ggcattgggc acggtgccgg
    10501 gctccgccgt gaggaacacg gccgtgcctt ccacgcgcat cggcgggctg acgaacaccg
    10561 cttccaggaa actcttgagg tcgatggcgt cggccttcag tttctcgccc agcagctcgc
    10621 ggccgcgctt ccaggtgatc atcagcatga agacggcgcc gccgatcaca agcgggaacc
    10681 agccgccctg ggcgatcttc agcaggttgg agctgaaaaa ggcgaggtcc accatgaaga
    10741 accagccggt ggcggcgacg cacagcgcca gcggatagtt ccagctgtag cggatcacga
    10801 agaaggtcag cgtcgtggtg atgagcatgt ccagcgtcac ggtgatgccg taggccgccg
    10861 ccaggttgct ggacgagcgg aacagcacca cggccaggac cacggcaacg aacaggcccc
    10921 agttgacgaa cggaatgtag atctggccgg tatcacgcac gctggtgtgg cggatctgca
    10981 ggcgcggcag gtagcccagc tggatcacct gcttggtgac gctgaaggcg ccggtgatca
    11041 tggcctggga ggcgatcacg gtagcggcag tggccagggc caccatcggc agcagggccc
    11101 agtccggcgc catcatgtag aaggggttct tcaccgcctc cgggttcttg agcagcagcg
    11161 cgccctggcc gaagtagttc agcgtgagcg ccggcatgac gatggcgaac caggccagcc
//
LOCUS       DASWIY010000283         2684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2045495, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000283 DASWIY010000000
VERSION     DASWIY010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2045495"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1167
                     /locus_tag="VGA59_10380"
     CDS             1..1167
                     /locus_tag="VGA59_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX9720127.1"
                     /translation="MDSETTFLGYRRENGRVGVRNHVIILPLDDLSNAAAQAVAHNIK
                     GALAIPHPYGRLQFGADLDLHFRTLIGTGCNPNVAAVVVIGIEDSWTKKVVDGIAATG
                     KPVSGFGIEGHGDHDTIMRASKAAREYVQWASEKQRERCAIRDLWVSTKCGESDTTSG
                     CGANPTVGNAFDKLHSLGNYLCFGETTELTGGEHIVAARCANDAVREKFMFMFNRYQD
                     VINRHKTSDLSDSQPTKGNIAGGLTTIEEKALGNIQKIGKSCTVDGVIDKAEVPTHPG
                     LWFMDSSSAAAEMVTLCAASGFAVHFFPTGQCNVIGNPILPVIKICANPRTVRLMSEH
                     IDVDTSGLLQREITLDQAGDRLLEMMLRTANGRLTAAEALGHREFVLTRLYESA"
     gene            complement(1175..1939)
                     /gene="moeB"
                     /locus_tag="VGA59_10385"
     CDS             complement(1175..1939)
                     /gene="moeB"
                     /locus_tag="VGA59_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX9720128.1"
                     /translation="MTDDQLLRYSRHILLEEIGVEGQERLMAAHALVIGAGGLGSPVA
                     LYLGTAGVGRITIVDHDTVDLTNLQRQIAHNMARIGEPKALSARDAIAAINPEPRVRG
                     LIARADAALLDDWVPQAHVVVDCCDNFSTRHLVNAACVKHGKPLVSGAAIRFDGQISV
                     YDTRDDKSPCYACVFAPSDDFEETLCATMGVFAPLVGIVGAMQAAEALKLLSGAGSSL
                     AGRLQMLDGRAMEWNEVRVPRNMACPVCKERPSARD"
     gene            2232..>2684
                     /locus_tag="VGA59_10390"
     CDS             2232..>2684
                     /locus_tag="VGA59_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9720129.1"
                     /translation="MTTQAKILIVDDEEVVRLSYMRILATSNCEVKAAWTWVQVAQAM
                     RHEPFDVVLLDLRMPDMDGMKVLRALKQSWPESEVIIITGYPTLESAKEAVSLGAYDY
                     LTKPVGPEQVITAANAAMLHKRWALHNAVDSRSEPSRQQSSPEVPGRAS"
BASE COUNT          527 a          881 c          861 g          415 t
ORIGIN      
        1 atcgacagcg aaacgacctt cctgggctat cgtcgcgaaa acggccgcgt gggcgtgcgc
       61 aaccacgtca tcatcctgcc gctggacgac ctgtccaacg cggcggccca ggccgtggcc
      121 cacaacatca aaggagcgct ggcgatcccg cacccctacg gccggctgca attcggcgcc
      181 gacctggacc tgcatttccg caccctgatc ggcaccggct gcaaccctaa cgttgccgcc
      241 gtcgtggtga tcggcatcga ggacagctgg acgaagaaag tcgtcgatgg catcgccgcg
      301 acgggcaagc cggtcagcgg gttcggcatc gaaggccacg gcgaccacga cacgatcatg
      361 cgcgccagca aggccgcgcg cgaatatgtg cagtgggcca gcgagaagca gcgtgaacgc
      421 tgcgcgatcc gcgacttatg ggtatcgacg aaatgcggcg agtccgacac cacctccggc
      481 tgcggcgcca accccacggt gggcaacgca ttcgacaagc tgcactcgct gggcaactac
      541 ctgtgtttcg gcgagaccac cgaactgacc ggcggcgaac acatcgtggc ggcgcgctgc
      601 gccaacgatg ccgtgcgcga gaaattcatg ttcatgttca atcgctacca ggacgtgatc
      661 aaccgccaca agaccagcga cctgtcggac tcgcagccga ccaagggcaa catcgcggga
      721 ggcctcacga ccatcgagga aaaggcgctg ggcaacatcc agaaaatcgg caagagctgc
      781 acggtggatg gcgtgatcga caaggcggaa gtgccaacgc accccgggct gtggttcatg
      841 gactcgtcca gtgccgcggc cgagatggtg acgctgtgtg cagcgtccgg cttcgcggtg
      901 catttctttc ccaccggcca gtgcaacgtc atcggcaacc cgatcctgcc ggtgatcaag
      961 atctgcgcca acccgcgcac ggtccgcttg atgagcgagc acatcgacgt ggacacctcg
     1021 gggctgctgc aacgcgagat cacgctcgac caggcgggcg acaggctgct cgaaatgatg
     1081 ctgcgcaccg ccaatgggcg gctcacggcg gccgaggcgc tgggccaccg cgaattcgtc
     1141 ctgacgcggc tgtacgagtc ggcctgaccc agcgtcaatc gcgggcggac ggccgttcct
     1201 tgcacaccgg gcaggccatg ttccgcggca cccgcacttc gttccattcc atggcccggc
     1261 cgtccagcat ctgcaggcgg ccggccagcg agctgccggc gccgctgagc agcttgagcg
     1321 cctcggctgc ctgcatggca ccgacgatgc ccaccagcgg cgcgaacacg cccatggtcg
     1381 cgcaaagcgt ttcctcgaaa tcgtccgagg gcgcgaacac gcaggcatag cagggcgact
     1441 tgtcgtcgcg tgtgtcgtag accgagatct gcccgtcgaa gcggatcgcg gccccggaga
     1501 ccagcggctt gccgtgcttg acgcatgccg cgttgaccag atgccgcgtg ctgaagttgt
     1561 cgcagcaatc gacaaccaca tgagcctgcg gcacccaatc atccagcagc gccgcgtcgg
     1621 cgcgtgcgat caggccccgc acgcgcggct cgggattgat cgcggcgata gcgtcgcgcg
     1681 ccgacagggc cttgggttcc ccgattcgtg ccatgttgtg tgcgatctgc cgctgcaggt
     1741 tggtgaggtc caccgtgtca tggtcgacga tcgtgatgcg cccaacgccg gccgtgccca
     1801 ggtaaagcgc caccggcgag cccaggccgc cggcgccgat gaccagcgcg tgcgcggcca
     1861 tcaggcgctc ctgtccttcg acaccgattt cctcgagcag gatgtggcgc gaatagcgca
     1921 gcagctggtc gtccgtcatg aagcgcattg tggcgtatgg gggcggtctg ccccccggcc
     1981 cggtgtatag aaacgagcgc acggcgggaa tcgcgtaagc cgttgaatta catggcaatt
     2041 tgggatcgct gtggcgaagg tgcataaaac ccatgtactt cgactcccat tgcagcgtgc
     2101 cggggagccg ccgaccggcg gcctgcgcat gcgctaagcc attgacttga aaggcaaaag
     2161 cgcagggggc cgccgagctg gcacggctga tgcgtagacc cgtgtgcata cgcaagaaca
     2221 gagtccgcaa catgaccacc caagccaaga tcctgatcgt cgacgatgaa gaagtcgtgc
     2281 gcctcagcta catgcgaatc ctggccacca gcaactgcga ggtcaaggcg gcatggacct
     2341 gggtccaggt cgcgcaggcg atgcgccatg agcccttcga tgtcgtgctg ctggacctgc
     2401 gcatgccgga catggacggc atgaaggtcc tgagggcgct caaacagagt tggcccgaga
     2461 gcgaagtgat catcatcacc ggctatccga cgctcgaatc ggccaaggaa gccgtttcgc
     2521 tcggcgccta tgactatctc accaagccag tcgggccgga acaggtcatc acagccgcga
     2581 acgccgcgat gctgcacaaa cggtgggcat tgcacaacgc ggtcgattcg cgaagcgaac
     2641 cgagccggca acagagttct cccgaagtac ccggccgggc ttcc
//
LOCUS       DASWIY010000284         3890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1674569, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000284 DASWIY010000000
VERSION     DASWIY010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674569"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VGA59_10395"
     CDS             <1..442
                     /locus_tag="VGA59_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase small subunit"
                     /protein_id="HEX9720130.1"
                     /translation="FLSNGPGDPEPCDYAIEATRELVESGYPTFGICLGHQIMALASG
                     AKTFKMKFGHHGANHPVKDLDSGRVSITSQNHGFAVDEKTLPANLRATHVSLFDGTLQ
                     GLARTDKPAFCFQGHPEASPGPHDIGYLFDRFTGLMDQAAGDKK"
     gene            528..3758
                     /gene="carB"
                     /locus_tag="VGA59_10400"
     CDS             528..3758
                     /gene="carB"
                     /locus_tag="VGA59_10400"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX9720131.1"
                     /translation="MPKRTDIKSILIIGAGPIIIGQACEFDYSGVQACNALREEGYKV
                     ILINSNPATIMTDPATADVTYIEPITWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     WHNGVLAKYQVELIGATPQAIDKAEDRQKFKEAMTKIGLGSARSGTAHSMEESWAVQK
                     TLGFPTVIRPSFTLGGTGGGIAYNSEEFEVICKRGLEASPTSELLIEESLVGWKEFEM
                     EVVRDKADNCIIICSIENLDPMGVHTGDSITVAPAQTLTDKEYQIMRNASIAVLREIG
                     VDTGGSNVQFAINPNDGRMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LRNEVTGGATPASFEPSIDYVVTKVPRFAFEKFPAADSRLTTQMKSVGEVMAIGRTFQ
                     ESFQKALRGLEVGVDGMNEKTQDREVLEEELGEPGPERIWYVGDAFAQGMSVDEVFEL
                     TKIDRWFLVQIEEIVKIELELEKMTLDQIDEPTLRLLKKKGFSDRRLARQLKTTDKVI
                     RERRRALGVRPVYKRVDTCAAEFATHTAYMYSTYEDECEADPTDRKKIMVLGGGPNRI
                     GQGIEFDYCCVHAALAMREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLEDVLEIV
                     DKEKPLGVIVQYGGQTPLKLALGLEAEGVPIIGTSPDMIDAAEDRERFQKLLHELKLL
                     QPPNATARTEAEALKKAAALGYPLVVRPSYVLGGRAMEIVHEQRDLERYMREAVKVSN
                     DSPVLLDRFLNDAIECDVDCLSDGKQTFIGGVMEHIEQAGVHSGDSACSLPPYSLSSE
                     TVDEMKRQTAAMARALNVVGLMNVQFAIQELGGKDVIYVLEVNPRASRTVPFVSKATG
                     IQLAKVAARCMVGQSLESQGITKEAVPPYFSVKEAVFPFVKFPGVDTILGPEMKSTGE
                     VMGVGKTFGEAFIKSQLGAGTKIPRPTDAVRKVFLTVKNSDKPRAVEVARQLAAQGFQ
                     MAGTKGTAAAINAAGIPCESVNKVTEGRPHVVDMIKNNEIVMVINTVEERRNAIADSR
                     AIRTSALLARVTTFTTIAGAEAAVEGMKYLDNLDVSSLQELHAQLV"
BASE COUNT          790 a         1233 c         1257 g          610 t
ORIGIN      
        1 gttcctttcc aacggcccgg gtgaccccga gccgtgcgac tacgccatcg aagccacgcg
       61 ggagctggtc gaatccggct atcccacctt cggcatctgc ctgggccatc agatcatggc
      121 cctggcttcc ggggcgaaga ccttcaagat gaagttcggg catcacggcg ccaaccatcc
      181 cgtgaaggac ctggacagcg gccgcgtcag catcacgagc cagaaccacg gcttcgccgt
      241 cgatgaaaag acattgcccg ccaacctgcg cgccacgcac gtcagcctgt tcgacggcac
      301 gctgcagggc ctggcccgca ccgacaagcc ggcgttctgt ttccagggcc atccggaagc
      361 gtcgccggga ccccatgaca tcggttatct gttcgatcgc ttcacgggcc tgatggacca
      421 ggccgccgga gacaagaagt aaatgtgccc ccacgttcac ggctacgccg tatctctgcc
      481 ccccgagggg gccttcgctc ccttggagcg gtcctgcgga atcgaaaatg cccaagcgca
      541 ctgacatcaa gagcatcctg atcatcgggg ccggtcccat catcatcggg caggcttgcg
      601 agttcgacta ctcaggggtg caggcctgca acgcgctgcg cgaggaaggc tacaaggtca
      661 tcctgatcaa cagcaatccg gccacgatca tgaccgaccc ggccacggcg gacgtcactt
      721 acatcgagcc gatcacctgg cagacggtcg aaaagatcat cgccaaggaa aggccggatg
      781 cgatcctgcc gaccatgggc ggccagacgg cgctcaattg cgcgctcgac ttgtggcaca
      841 acggcgtcct cgccaagtac caggtggaac tgatcggcgc cacgccgcag gccatcgaca
      901 aggccgagga tcggcagaaa ttcaaggagg cgatgacgaa gatcgggctg ggctcggcac
      961 gctccggcac cgcccattcg atggaagagt cctgggccgt gcagaagacc cttggctttc
     1021 ccacggtgat ccgcccgagc ttcacgctgg gcggcaccgg cggcggcatc gcctacaact
     1081 ccgaggagtt cgaggtgatc tgcaagcgcg gcctcgaagc ctcgcccacc agcgagctgc
     1141 tgatcgagga atccctggtc ggctggaaag aattcgagat ggaggtggtg cgcgacaagg
     1201 ccgacaactg catcatcatc tgctcgatcg agaacctgga cccgatgggc gtgcacaccg
     1261 gcgactcgat caccgtggcg ccggcgcaga cgctgaccga taaggaatac cagatcatgc
     1321 gcaacgcctc catcgcggtg ctgcgcgaga tcggcgtgga cacgggcggc tccaacgtgc
     1381 agttcgcgat caaccccaac gacgggcgca tgatcgtgat cgagatgaac ccgcgggtgt
     1441 cgcgctcgtc cgcgctcgcg tccaaggcca ctggatttcc gatcgccaag gtcgcggcca
     1501 agctggccgt cggctacacc ctggacgaac tgcgcaacga ggtcacgggc ggcgccacgc
     1561 ccgccagttt cgagcccagc atcgactatg tggtgacgaa ggtgccgcgt ttcgccttcg
     1621 aaaaattccc ggcggcggat tcgcgcctga cgacccagat gaagtcggtc ggcgaagtga
     1681 tggccatcgg ccgcaccttc caggagtcgt tccagaaggc cttgcgcggc ctggaggtgg
     1741 gcgtcgacgg catgaacgag aagacgcagg accgcgaggt gctggaagag gaactgggcg
     1801 agcccggacc cgaacgcatc tggtacgtgg gcgatgcgtt cgcccaggga atgagcgtcg
     1861 acgaggtctt cgaactcacg aaaatcgaca ggtggttcct ggtgcagatc gaggagatcg
     1921 tgaagatcga gctcgaactc gaaaagatga cgctggacca gatcgacgag cccacgctgc
     1981 ggttgctgaa gaaaaagggt ttctccgacc gccggctggc gcggcagctc aagacgaccg
     2041 acaaggtcat ccgcgaacgg cggcgagccc tcggtgtgcg cccggtgtac aagcgtgtcg
     2101 atacctgcgc cgccgaattc gcgacccaca cggcctacat gtactcgacg tacgaggacg
     2161 aatgcgaggc cgacccgacc gaccggaaga agatcatggt cctgggcggc gggcccaatc
     2221 gtatcggcca gggcatcgag ttcgactact gttgcgtcca cgccgcgctc gccatgcgcg
     2281 aagacggcta cgagacgatc atggtgaact gcaacccgga aacggtgtcg accgactacg
     2341 atacctccga ccgcctgtac ttcgagccgg tgacgctgga agacgtgctg gagatcgtgg
     2401 acaaggaaaa gccactgggc gtgatcgtcc agtacggcgg ccagacgccg ctgaagctcg
     2461 cgctgggcct ggaagccgag ggcgtgccga tcatcggcac ttcgcccgac atgatcgacg
     2521 ccgccgaaga ccgcgaacgc ttccagaagc tgctgcacga gctgaagctc ctgcagccgc
     2581 ccaacgccac ggcgcggacc gaggcggagg cgctgaaaaa agccgccgcc ctgggttatc
     2641 cgctggtggt tcggcccagt tacgtgctcg gcggccgagc gatggagatc gtgcatgagc
     2701 agcgcgacct ggagcgctac atgcgcgaag ccgtgaaggt ctccaacgat tctccggtgc
     2761 tgctggaccg gttcctgaac gacgcgatcg aatgcgacgt cgactgcctc tccgacggca
     2821 agcagacgtt catcggcggc gtgatggagc acatcgaaca ggccggcgtg cattcgggcg
     2881 actccgcctg ttcgctgccg ccttattcgc tgtcctcgga aaccgtggac gagatgaagc
     2941 ggcagaccgc cgcgatggcg cgggccctga acgtcgtggg gctgatgaac gtgcagttcg
     3001 cgatccagga gttgggcggc aaggacgtga tctacgtgct cgaagtcaac ccccgggctt
     3061 cgcgcaccgt gcccttcgtg agcaaggcga ccggcatcca gctggccaag gtggcggcgc
     3121 gctgcatggt gggccagtcg ctggaatcgc agggcatcac gaaggaagcg gtaccgcctt
     3181 atttcagcgt caaggaagcc gtattcccgt tcgtcaagtt tcccggcgtc gacacgatcc
     3241 tcggcccgga aatgaaatcc accggcgaag tgatgggggt gggcaagacc ttcggtgaag
     3301 ctttcatcaa gagccagctg ggtgcgggca ccaagattcc gcggccgacc gacgcggtgc
     3361 gcaaggtgtt cctgacggtg aaaaacagcg acaagccgcg cgccgtcgag gtcgcgcgcc
     3421 agctcgcggc ccagggtttc cagatggccg gcaccaaggg aaccgccgcc gcgatcaacg
     3481 ccgcgggcat cccctgcgag agcgtgaaca aagtcacgga aggccgcccc catgtggtgg
     3541 acatgatcaa gaacaacgag atcgtcatgg tgatcaatac cgtggaggag cgccgcaacg
     3601 cgatcgccga ctcccgggcc atccgcactt cagccctgct ggcgcgggtg accacgttca
     3661 cgaccatcgc cggcgccgaa gcggccgtgg aagggatgaa gtacctggac aacctggacg
     3721 tctcttcatt gcaggagctg cacgcccaac tggtttgaac tcgacggcgc gccgtcaggc
     3781 cccgtccctt ataatcgcgg catcgagaac accgccggtc ggctaggtcg gcggtgttcc
     3841 tttttgtgga gcattcagat ggcgacgatt ccgatcacgc ggcgaggagc
//
LOCUS       DASWIY010000285         6608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3494643, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000285 DASWIY010000000
VERSION     DASWIY010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3494643"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VGA59_10405"
     CDS             complement(<1..319)
                     /locus_tag="VGA59_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX9720132.1"
                     /translation="MAARRLQDLLLLLLAFLLILPVLALLASWLQWGGHGAQVLGEMA
                     RTVLPGYVWTSVGLCIVVGIGVALIGGATAAAVTLFDFPGRRTFEWALLLPLAMPAYV
                     VAYA"
     gene            382..1545
                     /locus_tag="VGA59_10410"
     CDS             382..1545
                     /locus_tag="VGA59_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720133.1"
                     /translation="MFVELKKLSVCYPGRPQPAVDEVSFGLRAGDIGVLIGPSGCGKT
                     TLLRAVAGLERVSAGEVRISGQVVGGAGIHVPAEARRIGMVFQDYALFPHIDVGRNVA
                     FGIHRLPKPERSARVAEVLELVGLDGIQRRFPHELSGGQQQRVALARALAPKPQLLLL
                     DEPFSNLDVDLRERLAHEIRGILKAAQATALFVTHDQLEAFAIGDVIGVVHEGRLQQW
                     DDAYTLYHRPATRFVADFIGHGVFAPATLREVNSQVVVQTPLGELRDMAECPLPCAFA
                     AGQCDVLLRADDIIHDDDAPVKAEILRKAFRGSEFLYTLRLASGQIVLAHVPSHHDHK
                     IGEWIGIRADVDHVVTFNRTCPIKDNEMCRLPCEHARPATVPAASGLAQAIDG"
     gene            complement(1516..2253)
                     /locus_tag="VGA59_10415"
     CDS             complement(1516..2253)
                     /locus_tag="VGA59_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9720134.1"
                     /translation="MNSLRAAWRLGRALVHALAGLATIALRFPRMTEIQRDAHVQSWA
                     LRMLPLLGIRLELRGRPPVTGPVLLVANHISWLDILVMHAARHCRFVSKADVRRWPLI
                     GTLATGAGTLYIERKSRRDAMRVVHHMAESLKRGEVVAVFPEGTTSNGVAMLPFHGNL
                     IQAAISANAPVQPVALSFRDGKTRAISLAPCYIDEDTLVMSIWRTLTAPAITAVVHYG
                     EPQSAQGRDRRTWANDLRSAVDGLREA"
     gene            complement(2262..3563)
                     /locus_tag="VGA59_10420"
     CDS             complement(2262..3563)
                     /locus_tag="VGA59_10420"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX9720135.1"
                     /translation="MKILIKNGRVIDPASNFDEICDVALAAGRVLAIKNIAPEFSASK
                     VIDATGCIVAPGLVDLAVRLREPGHEHEGMLESEMAAAVAGGVTSLVCLPDTDPVLDE
                     PGLVEMLKFRAEKLHQARVFPLGSLTRNLEGETLTEMVELTEAGCVGFSQAEVPLEST
                     QVLQRALQYAGTYGYTVWLRPQELHLGRGVAASGALATRLGLAGVPVAAETIALHTIF
                     ELMKSTGARVHLCRLSSAAGLELVRRAKDDGLAVTCDVSINSLHLTDVDIGYFDSRMR
                     LNPPLRQQRDRDALREALADGTIDALVSDHTPVSEDAKTLPFAEAEPGATGLELLLAL
                     ALKWGRENGVGLARSLAVLTSEPARVLGPSLGTLQASAGQLVEGGIADVCVLDPQAEW
                     IVTADALRSQGKHTPFSGYEMAGRVRCTIAAGQIAYEGWDR"
     gene            complement(3560..4522)
                     /locus_tag="VGA59_10425"
     CDS             complement(3560..4522)
                     /locus_tag="VGA59_10425"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX9720136.1"
                     /translation="MLYKKNPQLNKNGELVHLLTVEGLPRQILTHILDTAASFVSVSD
                     REVKKVPLLRGKSVFNLFFENSTRTRTTFEIAATRLSADVINLDIARSSASKGESLLD
                     TIANLSAMAADLFVVRHSESGAPFLIAQHVAPHVHVINAGDGRHAHPTQGLLDMYTIR
                     HYKKDFSNLTVAIVGDVLHSRVARSDIHALTTLGCAEVRVVGPKTLVPADMAQMGVRV
                     CHKLEEGIRGCDVIIMLRLQNERMSGALLPSSQEYFKSFGLTPEKLRLARPDAIVMHP
                     GPINRGVEIDSAVVDGGQSVILPQVTFGIAVRMAVMSMVAGTEA"
     gene            complement(4609..5106)
                     /gene="pyrR"
                     /locus_tag="VGA59_10430"
     CDS             complement(4609..5106)
                     /gene="pyrR"
                     /locus_tag="VGA59_10430"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX9720137.1"
                     /translation="MSELVLDAEALYRELLRGVQQLHTADTQLVGITSGGAWLAERLH
                     RDLGLPGEAGVISSAMHRDDFAKRGLAGAGQQTQLGFEVNDSHIVLLDDVLYTGRTIR
                     AVLNELFDYGRPASVKLAVLVDRGGRELPVQADYAAARVALPPTQSLELARDDAGKFS
                     FKVET"
     gene            complement(5162..5575)
                     /gene="ruvX"
                     /locus_tag="VGA59_10435"
     CDS             complement(5162..5575)
                     /gene="ruvX"
                     /locus_tag="VGA59_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX9720138.1"
                     /translation="MSTLVLDVPSHFQTFLAFDFGLKRTGVAVGNRLLRTATPQPTIR
                     AQGAAQLAEAEARAREWRPDAVVVGVPFHPDGASHDNTSRARKFARQLRGRLKLPVFE
                     VDERYSTTEALAAGAKDPDAGAACVILEQFLRSLP"
     gene            complement(5777..6376)
                     /locus_tag="VGA59_10440"
     CDS             complement(5777..6376)
                     /locus_tag="VGA59_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX9720139.1"
                     /translation="MPADAHSIDLTHHFLIAMPGMEDQAFARSVVYLCEHSARGALGL
                     VINKPSDINLRHLFDKVELPLGRDDLARTPVFQGGPVQTERGFVLHEAVFAADAAPNE
                     PVYASTMTIPGGLEMTTSKDVLEAISTGAGPRKVLVSLGYSAWGEGQLESEIAENSWL
                     TVGADPVVIFDTPVEQRYEKALSLLGLQSWMLSRDAGHA"
BASE COUNT         1061 a         2210 c         2246 g         1091 t
ORIGIN      
        1 aggcgtaggc gaccacgtat gccggcatcg cgagcggcag cagcagggcc cattcgaagg
       61 tgcggcggcc gggaaaatcg aacaaagtca cggccgccgc cgtcgcgccg ccgatgagcg
      121 cgacaccgat ccccacgacg atgcacaggc ccacgctggt ccatacatag ccgggcagta
      181 ccgtgcgggc catctcgccc aatacctggg cgccgtgccc gccccactgc agccaggacg
      241 ccagcaaagc caacaccggg aggatgagca ggaacgccag cagcagcagc aaaaggtctt
      301 ggagccggcg tgcggccatg gcgggaagct tgggaaagcc agtcgatgca aatgagaatt
      361 gtaatcatct acaatgagcc catgttcgtc gagctcaaga agctgtcggt ttgctatccg
      421 ggccggcccc agccagcggt ggacgaggtc tcgttcggcc tgcgcgcggg cgacatcggt
      481 gtgctgatcg ggccttccgg ctgcggcaag acaaccctgc tgcgcgcggt ggcgggcctg
      541 gagcgtgtgt ccgccggcga ggtgcgcatt tcggggcagg tcgtgggcgg tgccggcatc
      601 catgtgcctg ccgaagcccg gcgcatcggc atggtgttcc aggattacgc gctgttcccg
      661 catatcgatg tgggacgcaa tgtcgctttt ggaatccacc ggctgcccaa gcccgagcgc
      721 agcgcgcgtg tggccgaggt gctggaactg gtgggcctgg acggcatcca gcggcgcttc
      781 ccgcatgagc tttcgggcgg ccagcagcag cgcgtggcgc tggcgcgtgc gctcgcgccc
      841 aagccgcagt tgctgctgct ggacgagccc ttttcgaacc tggacgtcga cctgcgcgag
      901 cgcctggcgc acgagatacg aggcatcctg aaggccgcgc aggccacggc cttgttcgtg
      961 acgcatgacc agctcgaggc cttcgcgatc ggtgacgtga tcggcgtcgt gcacgagggc
     1021 cggctgcagc agtgggacga tgcctacacg ctgtaccacc ggccggccac gcgcttcgtc
     1081 gccgatttca tcggccatgg cgtcttcgcg ccggccacgc tgcgcgaggt gaacagccag
     1141 gtggtggtgc aaacaccgct gggcgagttg agggacatgg ccgagtgccc cctgccctgt
     1201 gccttcgcgg ccggtcagtg cgatgtgctg ctgcgcgccg acgacatcat ccacgacgac
     1261 gacgcgccgg taaaagccga gatcctgcgc aaggcgttcc ggggctccga gttcctctac
     1321 acgctgcggc tggccagcgg ccagatcgtg ctggcgcatg tgccgagcca ccacgaccac
     1381 aagatcggcg agtggatcgg catccgggcc gacgtcgacc atgtggtgac cttcaaccgc
     1441 acctgcccga tcaaggacaa cgagatgtgc cggctgccgt gcgaacacgc gcggcccgcc
     1501 accgtgccgg cggcatcagg cctcgcgcaa gccatcgacg gctgagcgca ggtcgttggc
     1561 ccaggtccgg cggtcgcgcc cctgcgcgct ctggggttca ccgtaatgga ccacggcggt
     1621 aatcgcgggc gcggtcaggg tccgccagat cgacatgacc agcgtgtcct cgtcgatgta
     1681 gcagggcgcc aggctgatgg cgcgcgtttt gccgtcccga aagctcagcg ccaccggctg
     1741 caccggcgcg ttggccgaga tggcggcctg gatcaggttg ccgtggaacg gcagcatggc
     1801 gacgccgttg ctggtcgtgc cttccggaaa aacagcaacg acctcgccgc gcttcaggct
     1861 ctcggccatg tggtgcacca cccgcatcgc atcgcgccgc gacttgcgtt cgatgtagag
     1921 ggtgcccgcg cccgtggcca aggtgccgat caggggccag cgccggacat cggccttgga
     1981 cacgaagcgg caatggcgcg ccgcatgcat gacgaggatg tcgagccatg agatgtggtt
     2041 ggccaccagc agcaccggcc cggtgactgg gggccggccg cgcagttcga gccggatgcc
     2101 cagcagcggc agcattctga gggcccagga ctgcacgtgc gcgtcgcgct ggatttcggt
     2161 catgcgcgga aagcgcagag cgattgtggc aaggccggcc aaggcatgca ccagcgcccg
     2221 ccccaaccgc cacgcggcgc gcagcgaatt catgggcgaa gtcagcggtc ccagccttcg
     2281 taggcgatct ggccggccgc gatcgtgcag cgcacgcggc cggccatttc atagccggag
     2341 aacggcgtgt gcttgccctg gctgcgcagc gcgtcggcgg taacaatcca ttcagcctgc
     2401 gggtcgagca cgcagacgtc ggcgattccg ccttcgacca gctggccggc gctggcttgc
     2461 agggtcccga gcgaggggcc gagcacgcgc gccggctcgc ttgtgagcac cgccagggac
     2521 cgagcgaggc caacgccgtt ttcccggccc cacttgagcg ccagtgccag cagcagttcc
     2581 aggccggtgg cgccgggctc ggcttccgcg aacggaagtg tcttggcgtc ctcgctcacc
     2641 ggcgtatggt ccgacaccag cgcatcgatg gtgccgtcgg ccagggcttc gcgcagggca
     2701 tcgcgatccc tttgttgcct gagcgggggg ttcaagcgca tgcggctgtc gaaatagccg
     2761 atgtccacgt cggtgaggtg cagggagttg atgctcacgt cgcaggtcac ggccagaccg
     2821 tcatccttgg cgcgccgcac cagctccagt ccggccgcgc tgctgaggcg gcacaggtgc
     2881 accctcgcgc cggtggactt catcagctcg aaaatcgtgt gcaaggcgat cgtttccgcg
     2941 gcgaccggca cacccgccag gcccaggcgc gtggccagcg cgccgctggc ggccacgccg
     3001 cggccaagat gcagctcctg cggccgcagc cagaccgtgt agccataggt gcccgcgtac
     3061 tgcagggcgc gctgcaacac ctgggtgctt tccagcggca cctcggcctg gctgaacccg
     3121 acgcatcctg cctccgtgag ttcgaccatc tcggtcagcg tttcgccctc gaggttgcgc
     3181 gtgagcgacc ccagcggaaa tacccgcgcc tggtgcagct tctcggcgcg gaacttgagc
     3241 atctcgacca gccccggctc gtccagcacc ggatcggtgt cgggcaggca gaccaggctg
     3301 gtcacgcccc cggccaccgc cgcggccatt tcgctttcca gcatgccctc gtgctcgtgc
     3361 ccaggttcgc gcaggcgcac ggcgaggtcc accaatcccg gcgcgacgat gcaaccggtc
     3421 gcgtcgatga ccttgctggc cgagaactcc ggcgcgatgt tcttgatcgc aagcacacgg
     3481 ccggcggcca gtgcgacgtc gcagatctcg tcgaaattgg aggccgggtc gatgacacgg
     3541 ccgttcttga tcaggatttt catgcttcag ttcccgcgac catgctcatc accgccatgc
     3601 gcaccgcgat accgaaggtg acctgcggca ggatcacgct ttgcccgccg tcgaccaccg
     3661 ccgaatcgat ttccacgccc cggttgatcg gccccggatg catgacgatg gcgtcgggcc
     3721 tggccagccg cagcttttcg ggcgtgaggc cgaaactctt gaaatactcc tgcgaggacg
     3781 gcagcagcgc gccgctcatg cgctcgttct gcagccgcag catgatgatc acgtcgcatc
     3841 cccggatgcc ttcttccagc ttgtggcaca cgcgcacgcc catttgcgcc atgtcggccg
     3901 gcaccagcgt cttcggcccg accacccgga cctcggcaca acccagcgtc gtgagcgcgt
     3961 ggatgtccga gcgcgcgacg cgcgaatgca gcacgtcgcc cacgatggcc accgtgagat
     4021 tggagaaatc cttcttgtag tgccggatgg tgtacatatc cagcagtccc tgggtcgggt
     4081 gggcgtgacg gccgtcgccc gcgttgatca cgtgcacatg cggcgccacg tgctgcgcga
     4141 tgaggaaggg cgcgcccgat tcgctgtggc gcaccacgaa caggtcggcc gccatcgccg
     4201 acaggttggc tatcgtgtcc agcagcgatt cgcccttgga ggccgaggag cgcgcgatgt
     4261 cgaggttgat cacgtcggcg gacaggcgcg tggccgcgat ctcgaaagtg gtgcgggtgc
     4321 gggtggagtt ctcgaagaac aggttgaaca cgctcttgcc gcgcagcagc ggcaccttct
     4381 tgacctcgcg gtcgctgacg ctgacgaagc tggccgcggt gtccaggatg tgcgtgagga
     4441 tctggcgcgg cagtccttcc accgtgagca ggtggacgag ctcgccgttc ttgttgagtt
     4501 ggggattttt cttgtacaac atagctcaaa ccgtccgacg ggccgcccct agggcggttt
     4561 gcgccccctt agggggcaga gcagcggcgt agccgcaaac gtgggggctc atgtttccac
     4621 cttgaagctg aattttccgg cgtcgtcgcg cgccagctcg agcgattgcg tggggggcag
     4681 cgccacgcgg gcggcggcgt agtccgcctg caccggcagc tcgcgcccgc cgcggtccac
     4741 cagcaccgcc agcttcacgc tggccgggcg tccgtaatcg aacagctcgt tgagcacggc
     4801 gcggatggtg cggccggtgt agagcacgtc gtccagcagg acgatgtgcg aatcgttgac
     4861 ctcgaagccc agctgggtct gctgccccgc gccggcgagg ccacgcttgg cgaagtcgtc
     4921 gcgatgcatg gccgaggaga tcacccccgc ctcgccgggc aagcccaggt cgcggtgaag
     4981 gcgctccgcg agccaggctc cgccggaggt gatgccgacc agctgggtgt ccgcggtgtg
     5041 cagctgctgc acgccgcgca gcagttcgcg gtagagcgct tccgcatcca ggaccagctc
     5101 gctcatttgg caaacctccg cgccgcgcgc gtgaagttgg aggggggaac ggtcccatgg
     5161 ctcatggcag actcctcaag aattgttcaa gaatcacgca ggcagcgccc gcgtcgggat
     5221 ctttcgcgcc ggccgccagc gcctcggtgg tgctgtagcg ttcatccacc tcgaacaccg
     5281 gcagtttcag ccggccgcgc agctgccgcg cgaatttcct cgcgcgcgac gtattgtcgt
     5341 ggctcgcgcc gtcggggtga aagggcacgc ccaccaccac cgcgtcgggc cgccattcgc
     5401 gcgcccgcgc ctcggcctcg gccagctggg ccgctccctg cgccctgatc gtgggctgcg
     5461 gcgtggccgt gcgcagcagc cggttgccca cggcgacgcc ggtgcgcttc aggccgaaat
     5521 cgaaagcgag aaaagtttgg aagtgggaag ggacatcgag caccagggtg ctcatgcgac
     5581 cgcctcggga aggcgaacca tggcgccacg cccggtagcc ggcaacgtac gcgcgctcgc
     5641 ggcggtcagt cccaggcaag ttctcatgcg ggtcgcctca gaaagatcgc gcaggctctt
     5701 ggcttccgcg ggttcggcga catgcatgca ttcggctggt cgatccccag caagctgggg
     5761 cccctcgccc cgcgcctcat gcatgcccgg catccctcga cagcatccac gactgcaggc
     5821 ccagcaagga cagcgctttc tcgtagcgct gctccaccgg cgtgtcgaag atcaccaccg
     5881 ggtcggcgcc cacggtcagc cagctgttct ccgcgatttc ggattccagc tggccttcgc
     5941 cccaggcgga atagccgagc gacaccagga ccttgcgcgg tccggcgccc gtggaaatgg
     6001 cttcgagcac gtctttggag gtggtcatct ccagcccgcc gggaatcgtc atcgtcgagg
     6061 cgtagaccgg ctcattgggc gcggcatcgg cggcgaagac ggcctcgtgc aggacgaagc
     6121 ctcgctcggt ctgcacggga ccgccctgga ataccggtgt gcgcgccagg tcgtcgcgcc
     6181 ccagcggcag ttcgactttg tcgaacaggt ggcgcaggtt gatgtcgctg ggtttgttga
     6241 tcaccagacc cagcgcgccg cgggcgctgt gctcgcacag gtacaccacg ctcctggcaa
     6301 aagcctggtc ttccataccc ggcatggcga tcaggaagtg atgcgtcagg tcgatcgaat
     6361 gtgcatccgc gggcataggg tagattttag cgccgccgca agacggcgcg ccgtttccgg
     6421 cacccgaaag cgcggcttcc tgcgcgatca ggccgtgatc gcggcggcgc tgtagtgctt
     6481 gaccaggctc agcagctctt cttccgaata gggcttgccg aggtaatggt cgacgccgag
     6541 ttccttggcg tgctcgcggt gcttttcggc gatgcgcgag gtgatcatga tgatctgcag
     6601 cttggcca
//
LOCUS       DASWIY010000286         4792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_920975, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000286 DASWIY010000000
VERSION     DASWIY010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920975"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..470
                     /locus_tag="VGA59_10445"
     CDS             99..470
                     /locus_tag="VGA59_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9720140.1"
                     /translation="MKPTAAPAPHDWWVICLCAEWCGVCREWRDSFEKLAAAHPDLHF
                     AWIDVEDEAQAMGDMDIETFPTMLVARGDSPRFFGPVQPSAAQLSRLLASLVEGPVPA
                     RELPQAAALLERLASAVLSKG"
     gene            621..848
                     /locus_tag="VGA59_10450"
     CDS             621..848
                     /locus_tag="VGA59_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEX9720141.1"
                     /translation="MANQGNKGSSNRGFASMDPQRQREIASEGGRAAHEKGTAHEFTS
                     EEAREAGRKGGQARSESNRSGSRSNSGSSSR"
     gene            complement(1072..1251)
                     /locus_tag="VGA59_10455"
     CDS             complement(1072..1251)
                     /locus_tag="VGA59_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720142.1"
                     /translation="MRFPAAAIKPKQPLRAAAAAELIRRIVPARELPPAEPAPLRNED
                     LPADLDQRVRLVGEW"
     gene            complement(1358..1894)
                     /locus_tag="VGA59_10460"
     CDS             complement(1358..1894)
                     /locus_tag="VGA59_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9720143.1"
                     /translation="MTIVADVMTLGVRTMSPSDTVSKAAQAMDELNVGVIPVCDGDKL
                     VGMVTDRDIVVRGVAQECDARTTKLADVMSTNVRCATEDQDVDEILGEMADSQIRRLP
                     VVDSQRRLVGIVTLGDIADKDPDNEVDVATSLADISSPAQPDRSGQSQASGSAGGGSA
                     SGKRSAMTKDELEQAVGR"
     gene            complement(2040..2858)
                     /locus_tag="VGA59_10465"
     CDS             complement(2040..2858)
                     /locus_tag="VGA59_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX9720144.1"
                     /translation="MATKSRFWADLTTRDFAQLDAASAVAVLPLGATEQHGPHLPLSV
                     DHRLVDGVIDACLPNLPAGLPVLFLPTQQVGYSPEHSRFAGTLTLSSATVMASWMEIG
                     EGVALAGLKKLLLLNSHGGQVSLMDIVARELRTHYDMLVYSCSWWNLPLGDTVNGLFS
                     AEEHRFGVHGGEIETSMMLALRPQDVVMGQARDFKSSSQVRAAAYPILGNGSSAKLGW
                     QMQDYNAQGAAGNAAAASADKGRAVIEAAGRQLALMLQELSALPLSTLVDQPPT"
     gene            complement(2984..>4792)
                     /locus_tag="VGA59_10470"
     CDS             complement(2984..>4792)
                     /locus_tag="VGA59_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX9720145.1"
                     /translation="KLFRGAGWNVIKLVWGSDWDGLFARDTTGALVRAFANTVDGQMQ
                     TFAAKDGRFNRDNFFGQNEELKRLAQGMTDEQIDRLKRGGHDLVKIHAAYTAAAAHRG
                     QPTVVLAHTKKGYGMGAAGQGKMTTHSQKKLDEGTLIEFRNRFNLPLSDEQARNLEFF
                     RPPADSAEMRYLHQQRAKLGGYMPRRLAASQPLTVPALATYAAFATGAAGKEMSTTMA
                     FVRLLGNLLKDPELGPRIVPIVADEARTFGMANLFKQVGIYSSVGQRYEPEDIGSVLS
                     YREALDGQILEEGISEAGAIASWTAAATSYSVHGFPMLPFYIYYSMFGFQRVGDAIWA
                     AADQRSRGFLLGATSGRTTLAGEGLQHQDGSSHLVAATIPNCRAYDPAFAGEMAVIVD
                     HGMREMLVEQRDCFYYVTLMNDNYAQPDLPAGVEAHVVRGCYKFKRFLARTGIGEATH
                     VPPSVTLMGSGAILIEVVKAAAELAGEGIHCDVYSVTSWSELARDGRACEQRMLAGKT
                     SEPAQHGSAAERHAPVPFVEQQLAGGEGPIVAASDYVRAVPEAIRAFIPPGRRYLTLG
                     TDGFGRSDTRAALREFFGVDAAHIVRAAKRALSLRA"
BASE COUNT          785 a         1605 c         1573 g          829 t
ORIGIN      
        1 tcaacgccat ttcgcatctg gaacacggcg actacgggca gtgggtgacc tactcctgaa
       61 gcgattgcct cagggcgcag cttgctaaac tggcggcaat gaagccgacc gcagcccccg
      121 cgccccacga ctggtgggtg atctgcctgt gtgccgagtg gtgcggcgtc tgccgcgagt
      181 ggcgtgactc gttcgagaag ctcgccgccg cgcatcccga tctccacttc gcatggattg
      241 atgtcgagga tgaagcgcaa gccatgggcg acatggacat cgaaaccttc ccgacgatgc
      301 tggtcgcgcg gggcgacagt ccccgcttct tcggccccgt gcagccgtcg gccgcgcagc
      361 tctcgcgcct cctggcgagc ctggtcgaag gtcccgtccc tgccagggag ctgccgcaag
      421 cggcggcgct cctggaacgg ctggcatcgg cggtgctatc caagggatag caaccaatcg
      481 taaccagaca aggtctcgtc ctacacagcc ctgcttttct tcctgacgag cgtttcagcg
      541 ccgcgaacgc tcaatcgggg ttatggccgg ctttagcgcc ggtcatgatt taaccacccc
      601 ttttttcagg agttccgatc atggcaaacc aaggcaacaa aggcagttcc aatcgcggct
      661 tcgcgtcgat ggacccgcaa cgtcaacgcg aaatcgccag tgaaggaggc cgggccgcgc
      721 acgaaaaagg aacggctcac gaattcacgt cggaagaagc acgcgaagcc ggtcgcaagg
      781 gtgggcaggc ccgcagcgag agcaaccggt ccggttcgcg cagcaacagc ggcagcagca
      841 gccgctgaac ccgcaagcgc gccacgcagg gcgccgcggc gccctttttt actgggccgg
      901 cgctcctcgc gtcaggaaga cttgtatggc aggaagttgc ccgagaggac cacgctaaca
      961 cggtcacgct cgggaatcgg gctcgcgact gatgtccatc cgtgccgtac acattgacga
     1021 tctcgtatgc caacggttcg gcacggccag cctggctgaa tcggaggctg cttaccactc
     1081 gccgaccagt cgcacacgct ggtcgagatc ggcgggaaga tcctcattgc gcagcggtgc
     1141 aggctcagcc ggcggcagct cccgcgcggg gacaatacgg cggatcagtt cggcagccgc
     1201 ggcggcgcgc agcggctgct tgggtttgat ggcggcggct ggaaatcgca tggcggctcc
     1261 tttcatgtgc cagtgtagga actaccgcgg tttcgcgctg tgacgtggct cacatcctcg
     1321 tgcagatgtc gccttccagt gggcttgaag caacaggtca gcggccgaca gcctgctcca
     1381 actcgtcctt cgtcatggcc gaacgcttgc cgctcgcgga gccgccgccg gcgctgccac
     1441 tggcctgcga ctgtccggag cggtccggct gggccggtga cgagatatcg gccagcgacg
     1501 tcgcgacgtc cacctcgtta tcaggatcct tgtccgcgat gtcccccagc gtcacgatgc
     1561 ccaccagccg ccgctggctg tccacgaccg gcagccggcg tatctggctg tcggccattt
     1621 cgcccaggat ctcgtccacg tcctggtcct cggtagcgca gcgcacattg gtgctcatca
     1681 cgtcggccag tttggtggtc ctggcgtcgc attcctgcgc cacgccgcgc accacgatgt
     1741 cgcggtcggt caccatgccc accagcttgt cgccgtcgca gaccgggatc acgccgacat
     1801 tgagttcgtc catggcctgg gcggccttgg acacggtatc cgatggagac atggtccgca
     1861 cgccgagggt catcacgtca gcaactattg tcatttcgcg ctccttggag aaaatgccgg
     1921 cggggcgccg ggcacttcac caatctagcg tttgggccgg cccccggtgt cggtcgtctc
     1981 gcaagctggc gtaggacgac gctgatggcg cgccaggccc gatgccgcgg gccgcggctt
     2041 caagtcggag gctggtcgac cagagtggac agcggcagcg cggacaactc ctgcaacatc
     2101 agggccagct ggcggcccgc cgcttcgatc acggcacggc ccttgtcggc cgaggccgcg
     2161 gcagcattgc cggccgcgcc ttgggcgttg tagtcctgca tctgccagcc cagcttcgcg
     2221 ctgctgccgt tgccgaggat agggtaggcg gcggcgcgca cctgcgagga cgacttgaaa
     2281 tcgcgggcct gtcccatcac gacgtcttgc ggccgaagcg ccagcatcat cgacgtttcg
     2341 atttctccgc catgcacgcc gaagcggtgt tcttcggcac tgaacaggcc gttcacggtg
     2401 tcgcccaggg gcaggttcca ccagctgcag ctgtagacga gcatgtcgta gtgcgtgcgc
     2461 agctcccgtg ccacgatgtc catcaggctg acctggccgc cgtgcgagtt gagcagcagc
     2521 agtttcttca ggcccgccag cgcgacgcct tcgccaattt ccatccagct tgccatcacg
     2581 gtggctgacg acagggtgag cgttccggca aaacgcgagt gctcggggct gtagcccact
     2641 tgctgcgtcg gcaggaaaag caccggcagg ccggccggca gattgggaag gcaggcgtcg
     2701 atgacgccat ccaccagcct gtgatccacc gacagaggga ggtgaggccc gtgttgttcg
     2761 gtcgcaccca gcggcagcac ggccacggcg ctggcggcat cgagctgcgc gaagtcgcgc
     2821 gtggtcaggt cggcccagaa gcgggatttg gttgccatga gcgcatatta atgcggcaga
     2881 caggtttgtc atccccacca ggcttgtcat cccgggcttg acccgggatc catgacttcc
     2941 tggattgcgg ggcagggccg gagcgtgaca agcgcggcgc gggtcaagcc cgcaatgaca
     3001 aggcgcgttt cgcggcccgg acgatatgcg cggcgtccac gccgaagaac tcccgcagcg
     3061 cggcgcgggt gtcgctgcgc ccgaagccgt cggtgcccag cgtcaggtag cggcggcccg
     3121 gcgggataaa agcgcggatg gcttccggca ccgcgcgcac gtaatcgctg gccgcgacga
     3181 tcggaccttc gccaccggcg agctgctgct cgacgaaagg cacaggcgca tgacgttccg
     3241 cggcggatcc gtgttgggcc ggttcggacg tcttcccggc aagcattcgc tgctcgcacg
     3301 cccggccatc gcgcgccagc tcgctccagc ttgtgacgct gtagacgtcg cagtggatgc
     3361 cctcgcccgc cagctctgcg gcggccttga cgacctcgat caggatcgcg cccgaaccca
     3421 tcaaggtgac gctcggcggg acatgcgtgg cctcgcctat cccggttcgc gccaaaaagc
     3481 gcttgaattt gtagcacccg cgcaccacgt gcgcttccac accggccggc aggtcgggct
     3541 gggcgtagtt atcgttcatc agcgtcacgt agtagaagca gtcccgctgc tccaccagca
     3601 tctcgcgcat gccgtggtcg acgatcacgg ccatctcgcc ggcgaaggcc gggtcatagg
     3661 ccctgcagtt cgggatggtg gccgccacca ggtggctgct gccgtcctgg tgctgcaggc
     3721 cctcgccggc cagcgtggtg cgccccgacg tcgcgcccag caggaagccg cgcgagcgct
     3781 ggtcggccgc cgcccagatc gcgtcgccga cgcgctggaa cccgaacatc gagtagtaga
     3841 tgtagaacgg cagcatcggg aaaccatgca cgctgtagct ggtcgctgcc gccgtccagc
     3901 tggcgatggc accggcctcg ctgatgcctt cttcgaggat ctgcccgtcc agggcctcgc
     3961 gataggacag caccgagccg atgtcctccg gctcgtagcg ctggcccacg ctggaataga
     4021 tcccgacctg cttgaacagg ttggccatgc cgaaggtgcg cgcctcgtcg gcgacgatcg
     4081 gcacgatgcg cggccccagc tccgggtcct tcagcaggtt gcccagcagc cgcacgaaag
     4141 ccatcgtggt ggacatctcc ttgccggccg cccccgtggc gaaggccgca taagtggcaa
     4201 gtgcgggcac ggtcaagggt tgcgaggcgg ccagccgccg cggcatatag ccgcccagct
     4261 tcgcgcgctg ctggtgcagg tagcgcatct ccgcgctgtc cgccggcggc ctgaagaact
     4321 caagattgcg cgcctgctcg tcggaaagcg gcaggttgaa gcggttgcga aactcgatca
     4381 gcgtgccctc gtccagcttc ttctggctgt gcgtggtcat cttgccctgg ccggccgcac
     4441 ccatgccgta gcccttcttg gtgtgtgcca ggacgactgt gggctggccg cggtgggccg
     4501 cagccgcggt ataggcggcg tggatcttca ccaggtcgtg cccgccgcgc ttcaagcggt
     4561 cgatctgctc gtcggtcatg ccctgggcca ggcgcttgag ctcctcgttc tggccgaaga
     4621 agttgtcgcg gttgaagcgc ccgtccttcg ccgcgaacgt ctgcatctgg ccgtccacag
     4681 tattggcgaa agcacgcaca agggcgccgg tcgtgtcgcg tgcgaacagc ccatcccagt
     4741 cgctgcccca gaccagcttg atcacgttcc agccggcgcc gcggaacagc tt
//
LOCUS       DASWIY010000287        14264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3496925, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000287 DASWIY010000000
VERSION     DASWIY010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 14264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14264
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3496925"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VGA59_10475"
     CDS             <1..728
                     /locus_tag="VGA59_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720146.1"
                     /translation="AYTQEHEKTDDFARRAMALREKRRQQPSDPASVWRRLSMPLAKA
                     GAAESVEIDCFQRVMVCSARGTESFDLVHWLGQVHQCTVRSHRFAGAQAHARDIATDY
                     VDLEGSTGEVVRRVVAMARNGLHIGLTQAFLPDYSHLIVLLSGESKRDADLLNMLVSS
                     CWRLAHRPVLVGVAPPGCSHAGLREVAREGGLVYLDCDLSSRVDAPALWAVTVPALDF
                     SLGAHPGGSPCHPQQASTPNPNK"
     gene            complement(763..1680)
                     /locus_tag="VGA59_10480"
     CDS             complement(763..1680)
                     /locus_tag="VGA59_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HEX9720147.1"
                     /translation="MKSSEQQTRVGLIGHGEVGKIFAAGLKGQIGVIGVSAWDLKFAE
                     PSVRDAELALASRAGVTAADSMQALCAASDLIISAVTASNTLAVAREAAAHIPAGTVF
                     LDLNSASPGTKQKAAALIDAAGAHYVEAGVMTSVPPYGIKVPMLLGGPGAAELAAVLA
                     RWGMDAKPVSDRLGVASAIKMCRSVMIKGLEALVIESYTTAREYGVEDHVLPTLAETF
                     PSIDWPEQGAYFFSRVVQHGKRRAEEMREAANTVREAGFEPFMSAAIAYKQQWVADQA
                     AGGVFQGLAKDAPWQEYADRLIAARPNWK"
     gene            complement(1739..3055)
                     /locus_tag="VGA59_10485"
     CDS             complement(1739..3055)
                     /locus_tag="VGA59_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9720148.1"
                     /translation="MSGIELAVWSFPVLLGLIFLRVPIGLAMLVCGFGGAYLVYGSIS
                     PILNQLKQVTYSTFSGYSLSVIPLFLLMGQFAAIGGLSAALFKAAEAWIGHRRGGVAM
                     AAIGACAGFGAICGSSIATAATMGQVTLPELKRNGYSGALATGCLAAGGTLGILIPPS
                     IILVIYAILTEQNIAKLFLAAFVPGILAMIGYMVVIAIYVRVSPGSAGRLPAVPMRER
                     LVQTWKVWPVLAIFIAVVGGIYGGIFTPTEGAAVGAAGTGLVAWVNRSLDRRRLVASL
                     LATAMSTGMIFMIVLGAGVYNTFLALSQLPQEGAQLVAAAGLSPWTVLTAILLLYLVF
                     GCVMDSLSMILLTIPIFFPIVSVLDFGLPPEEFAIWFGILVLIVVEVGLITPPVGMNL
                     FIINSIARETPILHTFRGVIPFVASDLIRVVILTLFPAITLFALRL"
     gene            complement(3052..3573)
                     /locus_tag="VGA59_10490"
     CDS             complement(3052..3573)
                     /locus_tag="VGA59_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9720149.1"
                     /translation="MMNIANSLARWLAVAGGVLLTLVMVLVVVSVSGRALLNFGLGPV
                     PGDFELVEMCTAVVVFFFLPWCYLRDGHAMVDLAYIHFPRPLQRAVTVFSDVLMLLAW
                     VVLTWRLGIAVGEKYAEQETTFILQWPLWPGYAAGFFGAVIGCFVYMAKTLSEFGLVK
                     RLEDPRISAGTHV"
     gene            complement(3647..4675)
                     /locus_tag="VGA59_10495"
     CDS             complement(3647..4675)
                     /locus_tag="VGA59_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9720150.1"
                     /translation="MHKRAFIKTAIAAAALFAAGHGFAQQVTLRLHQFLPPQATIPAK
                     ALVPWAQKVEKDSGGRIKVQLYHAMQLGGTPPQLFDQARDGIADLTWTVLGYTPGRFN
                     KSEVFELPFMTTTAEASSRAFHEYMEKFSADEFKDVKVIAVHTHGPGLFHTKEPVTGL
                     ESLHGMKIRGGSRIINNMLTKLGATPVGMPVPAVTEALSKGVISGTTIPWEVTPALKV
                     SQLVKNHTTFAGKYGLYTQTFVFAMNKNAYEKLPADLKKVIDDNSGVETAAMFGRAMD
                     QGDVAGRAIAVKAGNNIVALDVNETQRWRRTAAAVETDWISEMKGKGIDGAKLAAEAR
                     ALVEKHNK"
     gene            complement(4812..5516)
                     /locus_tag="VGA59_10500"
     CDS             complement(4812..5516)
                     /locus_tag="VGA59_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720151.1"
                     /translation="MTELLQVENLSAGYGEAVILDRVSLSLPAGETMALLGRNGTGKT
                     TLINTLAGATRQHGGTIRLAGVELHKLPSHERAAAGIGWVPQERNIFKSLTVQENLTA
                     VARPGPWTLARVYEMFPRLAERTHNLGTQLSGGEQQMLAVGRALAINPRLLLLDEPLE
                     GLAPIIVKELLRAIARITREEGLSAIIVEQHPQAILAISHRAVVLDHGAVVHSGAARD
                     LKEAPELLDRLLGVAR"
     gene            complement(5509..6288)
                     /locus_tag="VGA59_10505"
     CDS             complement(5509..6288)
                     /locus_tag="VGA59_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720152.1"
                     /translation="MNAGSQADEAVLSARGLVKRFGGIMATSNVSLDLRKGARHALIG
                     PNGAGKTTLVNLLTGVLDATEGTIALEGRDITHLPPHQRVRRGLVRTFQINQLFDSLT
                     PLETLALTVSQHQGVGAKWWQPLGARLAVAERCDQLLEQFHLTSVKDQPTRVLAYGKR
                     RLLEIAIALACEPRVLLLDEPVAGVPAGEREELLRTIANLPAEVSVLLIEHDMDLVFS
                     FATRMTVMVNGAVLTEGTPQEISSDARVKAVYLGQGDSRND"
     gene            complement(6285..7262)
                     /locus_tag="VGA59_10510"
     CDS             complement(6285..7262)
                     /locus_tag="VGA59_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9720153.1"
                     /translation="MKSGSGAQQSLLSGRRWRAWEPLLWLAAFAMPVLLPSHALIVNE
                     IAIVALFAISLDLVLGYTGIVSLGHAAFFGMGAYSAALFAKHIMPDPMVGLAVGIATA
                     TLLGAVFSVTIQRGSDLTRLMVTLGAALILMELANKLDWLTGGSDGLQGVIMGPLLGR
                     FEFDLSGRTAAWYSLTVLLVLFFLARRLVNSPLGATLKAIRDNRLRAMAIGIPVGSRL
                     SVSYTVAAGMAGAAGALLAQTTGFASLDLFEFHRSADVMLMLVVGGTGWLYGGVAGAI
                     VFKLMQDALSAITPQYWTFWLGLFLVVLVLVGRDRLIRPWTWFGKGRRA"
     gene            complement(7259..8134)
                     /locus_tag="VGA59_10515"
     CDS             complement(7259..8134)
                     /locus_tag="VGA59_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9720154.1"
                     /translation="MLTILFDGIAYGMLLFILAVGLAVTMGLMNFINLAHGAFAMAGG
                     YITVLLMQRLDVPFLWCLPLAFIGAALLGAVFERTLYRPLYHKPHLDQVLFSIGLTFM
                     AVAATDYFMGSTQQIIQLPSFLRGRTELGTGDWILGMGHYRLFIIAVCAALTVGLQFV
                     LARTRFGSRLRASVDDQRVAAGLGINVNVVFLSTFAFGSGLAGLGGALGAEVLGLDPT
                     FPLKFMIYFLIVVAVGGTSSITGPLLAALLLGIADVAGKYYIPKLGAFIVYSLMIVIL
                     VWRPQGLFVRRGGRP"
     gene            complement(8359..9531)
                     /locus_tag="VGA59_10520"
     CDS             complement(8359..9531)
                     /locus_tag="VGA59_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9720155.1"
                     /translation="MHKRDLIRAAIAAALLAASGLGLAQDNKFKIGLILPMTGPFAST
                     GKQIEAAARLYMAQNGDTVAGKKVELIVKDDTGTPDVTKRIAQELVVNDKVNVLAGFG
                     LTPLALATAPIATQSKTPLVVMAAATSSITQASPYIVRTSFTLPQVAVGIADWAPKNG
                     IKKVVTLVTDYGPGIDAEKFFKERLQFNGGQVVESLRVPLRNPDFAPFLQKVRDAKPD
                     ALFAFVPSGAGAALMKQFAERGLDKAGIRLIGTGDVTDDDILNSMGDVAVGVVTSHHY
                     SAAHNSSANRKFVEAFTKANNNLRPNFMAVGGYDGMRLIYEALKKTKGAGGGDALLAA
                     MKGQIFESPRGPMFIDAQTRDVVHNVYIRKVEKKDGQLYNVEFDVIKDVKDPGKAK"
     gene            9788..10363
                     /locus_tag="VGA59_10525"
     CDS             9788..10363
                     /locus_tag="VGA59_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX9720156.1"
                     /translation="MLKRRPPPAQSPPGRKISSIEDLERYLPAFLSWIASKLQRGASQ
                     HYLNVFDVGIETWRCLMLLAIENSISAQRVSRIIGMDKASISRCFKRMQARGLITMAL
                     DDADGRLRIAKLTAKGRKLHDQMIGIALERERALVEVLTPAELDTLIGLLQRLHENLP
                     AVEEATERYVAQRFPRAAGRRRARPIADEDE"
     gene            10560..11222
                     /locus_tag="VGA59_10530"
     CDS             10560..11222
                     /locus_tag="VGA59_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX9720157.1"
                     /translation="MSDWNDEERQLVEWMADMNEDDALALAKRMLVEEGKDPVRVLEL
                     CRSAMDIVGKRFEEGEYFLPELVLAGEMLETIGAIAKPLIQVKPGEESKKLGKVLIGT
                     VHGDLHDIGKNIVCFMLDINGFEVKDIGIDVPIDNFLGAIDEFKPDVVGLSGFLTLAF
                     DSMKETIAAIERAGRRDGLKIMIGGGQIDETVRAYTGADAFGVNAVEAVNLCKNWMGA
                     TA"
     gene            11219..12484
                     /locus_tag="VGA59_10535"
     CDS             11219..12484
                     /locus_tag="VGA59_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX9720158.1"
                     /translation="MNAPLEQTPVAKAAYDARWQRILDCVALKQPDRMPVAMYATFWL
                     GKYAGVSCRELMYDYEKTREIAERAVLEFDPDVYSTLLLSTTSGPTLEALGYKQLQWP
                     GHGVGDNQPFQYLDREYMKADEYDDFLFDPTGYYLHTYLPRVATAFEGFEELPIFPGL
                     HYFRLVAGIRAFAKPRVREAFEKVIKAAEEAHRFMQHHVAFTNRMTQLGYPVTNGSAA
                     VSPYDFIADYFRGARGMMTDIYRNRDKLLQMLDKASIFLLRMTIANAKGSGNPIVFIP
                     IHWAPDAFMSDKQFREFWWPPFRKMLIGLIDAGLIPMPLWEADCTKRLEIIKDVPAGK
                     CIYWFERTDMVKAFEVLGDVVALRGNLSPSMMTTGQPHEVDAAVRHLAENVFHKGGKL
                     ILDCAFGIPDETPVANVRAMFDAARKYAG"
     gene            12634..13320
                     /locus_tag="VGA59_10540"
     CDS             12634..13320
                     /locus_tag="VGA59_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720159.1"
                     /translation="MAVARGFDVLRQAQTAPLNGRPSPARTRLRAQALAMARDEALVH
                     AAFSYRIVALQAPAAPVLHACGESLEAPWLLPASGQLTALACGVCTLGPALERRVSEL
                     FAQRRASLAMALDELGNELLFDISRRAQDRMQAEVSRRGLTMSGELRAGDPGLALDAQ
                     GAVLRLACAESIGIKLGDGLLMHPLKSTSMVLGVGVDLPVSAWSRCDSCRSRARCGVR
                     LRAFAAAATA"
     gene            13358..>14264
                     /locus_tag="VGA59_10545"
     CDS             13358..>14264
                     /locus_tag="VGA59_10545"
                     /inference="COORDINATES: protein motif:HMM:NF036965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9720160.1"
                     /translation="MTQNPRVADAPTGHKLRFAHLEHAITAQAGDTLFQSARRNGVRI
                     VGACGGRGTCGTCAVHVVDGQVERSPHAAPGGETRAGRKKWQRACQLRALSDCTVEVA
                     PRSLAAVVRADVAGSANAEPLPLDPLVRSLDLSLAPASLVDPASDIERLRRALPDLDL
                     RLDLGALRQLPGVLRAGSWSLCARLRQVELIGVAPAGSASLGLAVDLGTTNVAAFLID
                     LQSGRRPASLAIENPQVAWGADVISRVNHAVAQPGGGDELREATITAINALAQDLCQA
                     VAATPRDIVDVVVCGNTAMQHLLLGL"
BASE COUNT         2571 a         4767 c         4616 g         2310 t
ORIGIN      
        1 tggcgtacac gcaggagcat gaaaaaacgg acgatttcgc gcgccgggcg atggcgctgc
       61 gcgagaaacg ccgccagcag ccgtcggacc cggcctccgt ctggcgccgc ctttccatgc
      121 cgctggccaa ggcgggcgct gcggaatccg tcgaaatcga ctgcttccag cgcgtcatgg
      181 tctgctcggc gcgaggcacc gaatcattcg atctggtgca ctggctcggc caggtccatc
      241 agtgcacggt gaggagccac cggttcgccg gcgctcaagc gcatgcgcgc gacattgcga
      301 cggactacgt cgatctcgaa ggcagcaccg gtgaggtcgt gcgaagggtc gtggcgatgg
      361 cccgcaatgg cctgcacatc gggctgacgc aggcattcct gccggactat tctcacctga
      421 tcgtcttgct gagcggagaa tcgaagcggg acgcggacct gctcaacatg ctggtgtcgt
      481 cttgctggcg gctggcccac cgcccggtgc tcgtgggagt cgctccgccc ggctgttccc
      541 atgccgggct gcgggaggtg gcgcgcgaag gtggactggt ctatctcgac tgcgacctca
      601 gcagccgcgt tgatgcaccg gctctgtggg cagtcacagt gcccgcgctg gacttcagcc
      661 tcggggccca tccgggcggc tcgccgtgcc atccccagca ggccagcacc ccgaatccga
      721 acaagtgatt gcgcgacgcg cgacgccacg ctccctggaa agtcacttcc agtttgggcg
      781 cgcggcgatc agccggtccg catactcctg ccatggcgcg tccttggcga ggccctggaa
      841 cacgccaccg gccgcctggt cggccaccca ttgctgcttg tacgcgatcg ccgccgacat
      901 gaagggctcg aagcccgcct cgcgcacggt gttggcggct tcccgcattt cctcggcccg
      961 gcgcttgcca tgctgcacga cgcggctgaa gaaataggcg ccctgctccg gccagtcgat
     1021 ggaagggaaa gtttcggcca gcgtgggtag gacatggtcc tccacgccgt attcgcgcgc
     1081 ggtcgtgtag ctctcgatca ccagcgcttc cagccccttg atcatgacgc tgcggcacat
     1141 cttgatggcc gaagcgaccc ccagcctgtc cgagacgggc ttggcatcca tgccccagcg
     1201 tgcgagcacg gccgcgagtt cggcagcgcc tggcccaccc aggagcatag ggaccttgat
     1261 gccgtacggc ggcacggagg tcatcacccc ggcttcgacg taatgcgccc cggctgcgtc
     1321 gatcaaagcc gctgccttct gcttggtgcc cggcgaagcc gagttcagat ccaggaacac
     1381 cgtgcccgcg ggaatatgcg cggccgcctc ccgcgcgacg gccagggtgt tggacgctgt
     1441 cacggcggaa atgatcaggt ccgacgcggc gcacagcgcc tgcatcgagt ccgcggccgt
     1501 cacgcccgcc cgcgatgcca gggcgagctc ggcgtcccgc acagaaggct cggcgaattt
     1561 caggtcccac gcactgacgc cgatcacgcc gatctggccc ttcaggccgg cggcgaaaat
     1621 cttgccaact tcgccatggc cgatcaggcc cacccgcgtt tgctgctcgc tcgatttcat
     1681 ggctgcccct tgctgacgtc cttactgctt ctgtatgtta gggcccggca accggacact
     1741 acaagcgcag cgcgaacagc gtgatcgccg ggaacagcgt caggatgacc acgcggatca
     1801 ggtcgctggc cacgaaggga atcacgccgc ggaaggtgtg caggatggga gtttcgcgcg
     1861 ctatcgagtt gatgatgaac aggttcatgc ccaccggcgg cgtgatcagc cctacctcga
     1921 cgacgatcag caccaggatg ccgaaccaga tcgcgaactc ctcgggcgga agcccaaagt
     1981 cgaggaccga gacgatcggg aagaagatcg ggatcgtcag caggatcatc gacagcgagt
     2041 ccatgacaca gccgaagacg aggtaaagca gcagtatcgc cgtcaacacc gtccatgggc
     2101 tcaggcccgc ggccgcgacg agctgcgctc cctcctgggg cagctgcgac agcgcgagga
     2161 aggtgttgta cacgccggcg ccgagcacga tcatgaagat catgccggtc gacatcgccg
     2221 tggcgagcag cgatgccacg agcctgcgcc ggtccaggct cctgttgacc catgccacca
     2281 ggccggtgcc ggccgcaccc acggctgcgc cctcggtcgg tgtaaaaatg ccgccgtaaa
     2341 tgccgccgac gaccgcgatg aaaatcgcca gcaccggcca cactttccac gtctgcacga
     2401 ggcgctcccg catgggaacg gccggcaggc ggccggcgct gcccgggctt accctgacgt
     2461 aaatcgcgat gaccaccata tagccgatca tggccagtat tcccggcacg aaagcggcga
     2521 ggaacagctt ggcgatattc tgttcggtca ggatagcgta gatcaccagg atgatggacg
     2581 gagggatcag aatgcccagg gtgccacccg ccgccaggca gccggtggcg agagcgcccg
     2641 aatagccgtt gcgcttcaat tccggcagtg tcacctggcc catggtggcc gccgtcgcga
     2701 tcgaggagcc gcagatggcg ccgaaaccgg cgcaggcgcc gatcgccgcc atggccacgc
     2761 cgccgcgacg gtggccgatc caggcctcgg ccgccttgaa cagtgcagcc gacagtccgc
     2821 cgatcgccgc gaactgaccc atcagcagga acagcggaat cacagaaagt gaatagcccg
     2881 agaaagtgct gtaagtcacc tgcttgagct gattgaggat gggcgaaatg ctgccgtaca
     2941 ccaggtaagc gccgccgaag ccgcaaacca gcatcgcgag gccgatcggg acgcgcagga
     3001 aaataaggcc cagcaacacg ggaaacgacc agaccgccaa ttcgatgccg ctcatacgtg
     3061 ggtccccgcg gagatccggg gatcctcgag ccgcttcacc agcccgaact ccgacagtgt
     3121 cttggccatg taaacgaagc agccgatcac cgcgccgaaa aagcccgctg cgtagcccgg
     3181 ccagagcggc cattgcagaa tgaaagtggt ttcctgctcg gcatattttt cgcctacggc
     3241 tatgccaaga cgccaggtca gcaccaccca ggccagcagc atcagcacgt cgctgaagac
     3301 ggtgacggcg cgctggagcg gccgtgggaa atgtatgtag gccaggtcca ccatggcgtg
     3361 accgtcacgc aggtagcacc acggcaggaa gaagaacacc accaccgccg tgcacatctc
     3421 caccagctcg aaatcgccgg gcaccgggcc caggccgaaa ttgagcagtg cacggccact
     3481 gacgctcacc accaccaaca ccatcaccag ggtcagcagc accccgccgg cgacggccag
     3541 ccaacgcgca agggaatttg caatgttcat catggcatat gaagcggggc acccaggtgc
     3601 cccgctgtgt tgtcaccgag agagaaagaa aaccccgtcg cgcagtctac ttgttgtgtt
     3661 tttcgaccag tgcacgggcc tccgccgcca gcttggcgcc gtcgatgccc ttgcccttca
     3721 tctcgctgat ccagtcggtc tcgacggccg cggccgtgcg gcgccagcgc tgcgtctcgt
     3781 tcacgtccag cgccacgata ttattgccgg ccttgacggc gatcgcgcgc cccgcgacgt
     3841 cgccctggtc catggcacgg ccgaacatcg cggccgtttc cacgcccgag ttgtcgtcga
     3901 tgaccttctt caggtcggcc ggaagcttct cataagcgtt cttgttcatg gcaaagacga
     3961 acgtctgggt gtacagcccg tatttgccgg cgaacgtggt gtggttcttg accaactgcg
     4021 acaccttcag cgccggcgtg acttcccagg gaatggtagt gcccgagatc acgcccttgg
     4081 acagcgcctc ggtcaccgcc ggcaccggca tgcccaccgg cgtggcgccc agcttggtca
     4141 gcatgttgtt gatgatgcgc gagccgccgc ggatcttcat gccgtgcagc gattccaggc
     4201 cagtgaccgg ctccttggtg tggaacaggc cgggcccgtg cgtgtgcacg gcgatgacct
     4261 tcacgtcctt gaactcgtcg gcggagaatt tttccatgta ctcgtgaaat gcgcgcgagc
     4321 tggcttccgc cgtggtggtc atgaacggca gttcgaacac ctcggacttg ttgaatcttc
     4381 ccggggtgta gcccagcacc gtccaggtga ggtccgcgat gccgtccctg gcctggtcga
     4441 acagctgcgg cggggtgccg cccagctgca tggcgtggta aagctgcacc ttgatcctgc
     4501 cgccggagtc cttttcgacc ttctgagccc aggggaccag cgccttcgcc ggaatggtgg
     4561 cctggggagg cagaaactgg tgcaggcgca gggtgacctg ctgggcgaag ccgtggccgg
     4621 ccgcgaacag cgcggcggcg gcgatggcgg tcttgatgaa agctctcttg tgcatggcag
     4681 tctccggagg gttgaacatt ggacaggaca aacgggaccg attgcggtga ccggcctggg
     4741 gggccattgt cacccggatg ggttgcgagc ggaagggcta tcgcacacaa gcgcacggcg
     4801 aatccgctcc cttagcgtgc cacgcccagc agccggtcca gcagctcggg cgcctccttc
     4861 aggtcccgcg cggcgcccga atgcacgacg gcgccatgat cgagcaccac ggcgcggtgc
     4921 gagatggcca ggatcgcctg cgggtgctgc tcgacgatga tggccgacag tccttcttcg
     4981 cgcgtgatcc gggcgatcgc cctgagcagt tccttgacga tgatcggcgc caggccttcc
     5041 agcggttcgt ccagcaacag caggcgcggg ttgatcgcca atgcgcgccc caccgccagc
     5101 atttgctgct cgccgcccga caactgcgtt cccaggttgt gcgtacgctc ggcgaggcgc
     5161 gggaacatct cgtacacacg cgcaagcgtc cacggaccgg ggcgcgccac tgcggtcagg
     5221 ttctcctgca ccgtcagcga tttgaagatg ttgcgctctt gcggcaccca gccaatgccg
     5281 gccgccgccc gctcgtgaga gggtaatttg tgcagctcga cgccggccag cctgatggtg
     5341 ccgccgtgct gccgcgtggc gcccgcgagc gtattgatca gggtggtctt gcccgtgccg
     5401 ttgcgcccca gcagcgccat ggtttcgccc gccggcagcg acagcgacac ccggtcgagg
     5461 atgactgcct cgccgtagcc ggcgctcagg ttctcgacct gcagcaactc agtcattgcg
     5521 gctgtctcct tgcccgaggt agaccgcctt gacgcgagcg tcgctcgaga tctcctgcgg
     5581 cgtgccttcc gtgagcacgg cgccgttgac catcaccgtc atgcgcgtgg cgaagctgaa
     5641 cacgaggtcc atgtcgtgct cgatcagcag caccgagacc tcggccggca ggttggctat
     5701 ggtgcgcaac aattcctcgc gctcaccggc aggaacgccg gcgaccggct cgtcgagcag
     5761 cagcacgcgc ggctcgcagg ccaaggcgat ggcgatctcc agcagccggc gcttgccgta
     5821 ggccagcacc cgggtgggct ggtccttcac gctggtcagg tgaaactgct ccagcaactg
     5881 atcgcaacgc tcggccacgg ccagccgcgc gcccagcggc tgccaccatt tggcaccgac
     5941 gccttggtgc tgcgacacgg tgagcgccag cgtttcgagc ggcgtgaggc tgtcgaacaa
     6001 ctggttgatc tggaaggtgc gaaccaggcc ccggcgcacg cgctggtgcg gcggcagatg
     6061 cgtgatgtcc cggccttcca gcgcgatcgt cccttccgtg gcgtcgagca cgccggtcag
     6121 cagattgacc agcgttgtct tgcctgcgcc gttggggccg atcagcgcgt ggcgagcgcc
     6181 cttcctgaga tccagggaca cattgctggt ggccatgatg ccgccgaagc gcttgaccag
     6241 gccccgcgcg ctcagtacgg cctcgtctgc ctggctcccg gcattcatgc ccgccgtccc
     6301 ttgccaaacc aggtccaggg cctgatcagg cgatcgcggc cgaccaggac cagcaccacg
     6361 aggaacagtc cgagccagaa agtccagtat tggggcgtga tcgccgacag cgcgtcctgc
     6421 atcagcttga agacgatcgc gccggccacg ccgccataga gccagccggt gccgcccacc
     6481 accagcatca gcatcacgtc ggcggagcgg tggaactcga acaggtccag cgaggcgaac
     6541 cctgtcgtct gcgccagcag cgcgccggcg gcgcccgcca tgccggccgc caccgtgtac
     6601 gacaccgaca ggcgcgatcc gaccggaatg ccgatcgcca tcgcgcgcag ccggttgtcg
     6661 cggatcgcct tcaaggtcgc gcccagcggc gaattcacca ggcgccgcgc caggaagaac
     6721 agcaccagca gcaccgtaag cgaataccag gcggccgtgc gtcccgaaag atcgaactcg
     6781 aagcggccca gcagaggtcc catgatcaca ccctgcaaac catccgagcc gcccgtcagc
     6841 cagtccagct tgttggccag ctccatcagg atcagggccg ccccgagcgt caccatcagc
     6901 cgggtcaggt cgctgccgcg ctggatggtc acggaaaaga ccgcaccgag caatgtcgca
     6961 gtcgcaatgc ccaccgcgag ccccaccatc ggatccggca tgatgtgctt cgcgaacagc
     7021 gccgcggagt aagcccccat gccgaagaac gcggcatggc cgagcgacac gatgccggtg
     7081 tatcccagca ccaggtccag cgaaatcgcg aacagcgcca cgatggcgat ttcattgacg
     7141 attaaggcat ggctgggcag cagcaccggc atcgcgaaag ccgccagcca cagcagcggc
     7201 tcccaggcgc gccagcggcg gccggacaac agcgattgct gcgcgccgga tccactcttc
     7261 acggcctgcc ccctctgcgc acgaacaggc cctgcggccg ccacaccagg atcacgatca
     7321 tcaggctgta gacgatgaag gcgccgagct tggggatgta atacttgccg gccacgtcgg
     7381 cgatacccag cagcagcgcc gccagcagcg gcccggtgat ggacgaggtg ccgccgaccg
     7441 cgacaacgat caggaaatag atcatgaact tgagcggaaa agtcgggtcc agccccagca
     7501 cctcggcacc cagcgccccg cccagacccg ccaggccgga accgaaagcg aacgtggaga
     7561 gaaacacgac gttgacgttg attcccaggc cggcggccac ccgctggtcg tccaccgagg
     7621 cccgcaagcg gctgccgaag cgcgtgcgcg ccagcacgaa ttgcagcccc accgtgagcg
     7681 cggcgcagac ggcgatgatg aagaggcggt agtggcccat gcccaggatc cagtctcccg
     7741 tgccgagctc ggtgcgcccg cgcaggaacg aaggcagctg aatgatctgc tgggtggacc
     7801 ccatgaagta gtcggtcgcg gccacggcca tgaacgtcag gccgatggag aacagcacct
     7861 ggtccaggtg cggcttgtgg tacagaggcc ggtacagcgt gcgttcgaac acggcgccca
     7921 gcaacgccgc gccgatgaag gccagcggca ggcaccagag aaacggcacg tcgaggcgct
     7981 gcatcagcag caccgtgatg tagccgcccg ccatggcgaa tgcgccgtgg gccaggttga
     8041 tgaagttcat caggcccatg gtcaccgcca ggccgacggc aaggatgaac agcagcatgc
     8101 cgtaggcaat gccgtcaaaa aggatggtca acatggacga aaggacgtgg tggcgggacg
     8161 gggctgggta cagggcactg tcatgccggg ctcgacccgg catccatcgc ggccgctgct
     8221 caccgccatg gattgcgggg ccgggccgga gcgcaatgcc tcgggacgtg catagggtag
     8281 gcccggcatt gggctcgctc cactgtacag ctgcgcgcac ggacgttgca cgagcccagc
     8341 ccgcaatgac aaacctgcct acttggcttt gcccgggtcc ttgacgtcct tgatcacgtc
     8401 gaactccacg ttgtacagct ggccgtcctt cttttccacc ttgcgaatgt agacgttgtg
     8461 caccacgtcg cgcgtctggg catcgatgaa catcgggccg cgcgggctct cgaaaatctg
     8521 gcccttcatg gccgccagca gcgcatcgcc gccgcccgcg cctttggtct ttttcagcgc
     8581 ttcgtagatc agccgcatgc cgtcgtagcc gccgacagcc atgaagttgg gacgcaggtt
     8641 gttgttcgcc ttggtgaagg cctcgacgaa cttcctgttg gccgacgagt tgtgggcggc
     8701 ggagtaatga tgcgaagtca cgacccccac ggccacgtcg cccatgctgt tgaggatgtc
     8761 gtcgtcggtc acgtcccccg tgcctatgag cctgatgccg gccttgtcca ggccgcgctc
     8821 ggcgaactgc ttcatgagcg ccgcaccggc accggaaggc acaaaggcga acagcgcgtc
     8881 cggcttggca tcgcgcacct tctgcaggaa cggcgcgaag tccggattgc gcagcggcac
     8941 gcgcaggctc tcgacgacct gaccgccgtt gaactgcagc cgctccttga agaatttctc
     9001 ggcgtcgatg cccggaccgt aatcggtcac cagggtcacg actttcttga tgccgttctt
     9061 gggcgcccag tcggcgatac cgacggcaac ctgcggcagc gtgaagctgg tacgcacgat
     9121 gtacggcgaa gcctgggtaa tgctggaagt cgccgcagcc atcacgacca gcggcgtttt
     9181 cgactgcgtg gcgatgggcg cggtagccag tgccaacggc gtcaggccga agccggccag
     9241 cacgttgacc ttgtcgttga cgaccagctc ctgcgcgatg cgcttggtca cgtcgggcgt
     9301 gcccgtatcg tccttgacga tcaactcgac cttcttgccg gccaccgtgt cgccgttctg
     9361 cgccatgtac agcctggccg cggcctcgat ctgcttgccg gtggaggcga acggccccgt
     9421 cattggcagg atcaggccga tcttgaactt gttgtcctgg gcgaggccga ggccgctggc
     9481 agccagcaaa gcggcggcga tggcggcccg gatcaagtct ctcttgtgca tggagtctcc
     9541 ttcagaattg ctgcggtccg cggtcacagg aacacctgct ccgggaacag ccgtgattct
     9601 gttccctcgc cgtcccgaac gcaacgcgcc gccccgtcta gcagctatct gttttcccta
     9661 tatccaggca ggctgcggga taaccactag atggttgcca tcccaactgg ttgccaaacc
     9721 aactgcgcgc gtctatcgtt ggcgggaatc catttgacgg gtggctttgc ctatgaccgc
     9781 ggtgaccatg ctgaagcgac gcccacctcc tgcccagagc ccacccggcc ggaagatctc
     9841 cagcatcgag gatctcgagc gctacttgcc ggcgttcctg agctggattg ccagcaagct
     9901 gcagcgcggc gcatcgcagc attacctgaa cgtgttcgat gtcggcatag aaacctggcg
     9961 ctgcctgatg ttgctggcca tcgagaattc gatctcggct cagcgggtat cgcgcatcat
    10021 cggcatggac aaggcttcga tcagccgctg cttcaagcgc atgcaggcca ggggcctgat
    10081 cacgatggcg ctggacgatg cggacgggcg gctgcgcatc gccaagctca cggccaaggg
    10141 gcgcaagctg cacgaccaga tgatcggcat cgcgctcgag cgcgaacgcg ccctcgtcga
    10201 agtgctcacc ccggccgagc tcgatacgct gatcggcctg ctccagcgcc tgcacgaaaa
    10261 cttgccggcg gtcgaggaag cgacggagcg ctacgtcgcg cagcgtttcc cgagagccgc
    10321 cggacgccgg cgtgctcgtc ccatcgcgga cgaagacgag tgagctcgcc ggcatcacca
    10381 cagcagatgg gacgaggatc gggttaacca ctagccgcgt tagcgcaaag ccttgagatc
    10441 aattggttga catgccaacc aattgaggct accgtgatat tcactcgaat ggtttccgag
    10501 cgcattcgag agcgagccgc tacgcgagcc cggataccac gcctcaagga gacaagccca
    10561 tgagcgactg gaacgacgaa gagcgccaac tcgtcgaatg gatggccgac atgaacgagg
    10621 acgacgcgct ggcgctcgca aagcgcatgc tggtcgagga aggcaaggac ccggtgcggg
    10681 tgctcgaact gtgccgctcg gcgatggaca tcgtcggcaa gcgtttcgag gagggcgaat
    10741 acttcctgcc cgaactggtg ctggccggcg agatgctcga gaccatcggt gccattgcca
    10801 agcctttgat ccaggtcaaa cccggcgaag agagcaagaa gctcggcaag gtgctgatcg
    10861 gcaccgtgca cggtgacctg cacgacatcg gcaagaacat cgtctgcttc atgctcgaca
    10921 tcaacggctt cgaggtcaag gacatcggga tcgacgtgcc gatcgacaat ttcctcggcg
    10981 caatcgatga gttcaagccc gacgtcgtcg gcctgtccgg cttcctcacg ctggcgttcg
    11041 actcgatgaa ggaaaccatc gccgcgatcg agcgcgcagg gcggcgcgac gggctgaaga
    11101 tcatgatcgg cggcggccag atcgacgaaa cggtgcgcgc ctacaccggt gccgacgcgt
    11161 tcggcgtcaa cgccgtcgag gccgtcaacc tgtgcaagaa ctggatggga gcaacggcgt
    11221 gaacgccccc ctggagcaga cccccgtcgc caaggccgcc tacgatgcgc gctggcagcg
    11281 catcctggat tgcgtcgcgc tgaagcagcc cgaccggatg cccgtggcga tgtatgccac
    11341 tttctggctg ggcaagtacg ccggtgtcag ctgccgtgag ctgatgtacg actacgaaaa
    11401 gacccgggaa atcgccgaac gcgcggtgct cgaattcgat ccggatgtct acagcacctt
    11461 gctcctgtcg accacctcgg ggcccaccct ggaggcgctg ggctacaagc aactgcaatg
    11521 gcctggacac ggggtcggcg acaaccagcc gttccagtac ctcgatcgcg aatacatgaa
    11581 ggccgacgaa tacgacgact ttctgttcga ccccaccggc tactacctgc acacgtacct
    11641 gcctcgggtg gccaccgctt tcgagggttt cgaagaactg ccgatctttc cgggcctgca
    11701 ttatttccgc ctggtcgccg gtattcgggc gttcgccaag ccgcgagtgc gcgaagcctt
    11761 cgagaaggtc atcaaggcag ccgaagaagc ccatcgcttc atgcagcatc acgtggcgtt
    11821 caccaaccgc atgacccagc tcggctaccc ggtgacgaac ggctcggccg ctgtttcgcc
    11881 ctacgacttc atcgccgact actttcgcgg cgcccgcggc atgatgaccg acatctaccg
    11941 caaccgggac aagctgctgc aaatgctcga caaggcgtcg atcttcctgc tgcgcatgac
    12001 catcgccaat gccaaagggt cgggcaaccc gatcgtgttc atcccgatcc actgggcgcc
    12061 cgacgccttc atgtcggaca agcagttccg cgaattctgg tggccgccgt ttcgcaagat
    12121 gctgatcggc ttgatcgacg ccgggctgat cccgatgccc ttgtgggaag ccgactgcac
    12181 caaacggctg gagatcatca aggacgtgcc ggccggcaaa tgcatttact ggttcgagcg
    12241 caccgacatg gtcaaggctt tcgaggtgct gggcgacgtg gtcgccttgc gcggcaatct
    12301 gtcgccctcg atgatgacca ccggccagcc gcacgaagtg gacgcggccg tgcggcacct
    12361 ggccgagaac gtcttccaca agggcggcaa gctgatcctc gactgcgcat tcggcattcc
    12421 cgatgaaacc ccagtggcga acgtgcgggc catgttcgac gcggcgcgca aatacgcggg
    12481 ttgaagtgaa agcccacccc cgaagcgcct tcggcgcctc cccctcaagg gggcgccacc
    12541 agcggcccgg caaagccggt tccgcggtgg cccccgcctg gacccgggga tttcatgcgt
    12601 cgcgggtgaa gcgcggcatc gcggagaact gagatggccg tggcccgcgg cttcgacgtc
    12661 ctgcgccagg cgcagacggc gccgctcaac gggcggccct cgcctgcacg cacacgctta
    12721 cgcgcacagg ccctggccat ggcacgcgac gaagcgctcg tccacgccgc tttcagctac
    12781 cgcatcgtgg cgttgcaggc cccggcggca ccggtgctgc acgcgtgcgg cgaatcgctg
    12841 gaggcgccct ggctgctgcc ggcctcgggc cagctgaccg ccctggcctg cggtgtgtgc
    12901 accctgggtc cggcgctgga gcggcgcgtg agcgagctgt tcgcgcagcg ccgcgcctcg
    12961 ctcgccatgg cgctggacga actgggcaac gagttgctgt tcgacatctc gcgccgggcc
    13021 caggaccgca tgcaggccga ggtcagccgg cgcggcctga ccatgagcgg cgagttgcgc
    13081 gccggcgacc ccgggctggc gctggatgcg cagggcgccg tgctgcgcct ggcctgcgcg
    13141 gaaagcatag gcatcaaatt gggcgacggc ttgctgatgc atcccttgaa gtccacgtcg
    13201 atggtcctgg gcgtgggggt ggacttgccc gtgagcgcct ggtcgcgctg cgactcttgc
    13261 cgctcgcgcg ccagatgcgg cgtgcgtttg cgcgcgtttg ccgcggcggc aacggcctga
    13321 gaaacgccct ccggccgtcc agggaagctt gcccaccatg acccagaatc cgcgtgttgc
    13381 cgatgccccg acggggcaca agctacgctt tgcgcatctg gaacatgcca ttaccgcgca
    13441 ggccggcgac accctgttcc agagcgcacg gcgcaacggg gtgcggattg tcggtgcctg
    13501 cggcggacgc ggcacctgcg gcacctgcgc ggtccatgtc gtcgacggcc aggtcgagcg
    13561 cagcccccac gccgcgcccg gcggcgagac gcgcgcgggg cgcaagaagt ggcaacgcgc
    13621 ttgtcagctg cgggccttgt cggactgtac ggtcgaggtc gcaccacgct cactggcagc
    13681 ggtggtgcgc gccgatgtgg cgggcagcgc aaacgccgag ccgctgccgc tggacccgct
    13741 ggtgaggagc ctggacctga gcctggcccc ggctagcttg gtggacccgg ccagcgatat
    13801 cgagcggctg cgccgcgccc tgcccgacct ggatttgcgc ctggacctgg gcgcgctgcg
    13861 gcaacttccc ggtgtgctgc gcgcaggctc atggtcgctg tgcgcgcgcc tgcggcaggt
    13921 cgaactgatc ggtgtggccc ctgccgggag cgcgagcctg ggcctggcgg tggacctggg
    13981 caccaccaat gtggctgcgt tcctgatcga tctgcaaagc ggccggcggc ccgccagcct
    14041 cgccatcgag aacccgcaag tggcctgggg tgcggacgtg atcagccggg tcaaccacgc
    14101 cgtggcccag cccggcggcg gcgacgaact gcgcgaagcc acgatcaccg cgatcaatgc
    14161 gctggcccag gacctgtgcc aggccgtggc cgccacgccc agggacatcg tggacgtggt
    14221 ggtctgcggc aacacggcga tgcagcatct gctgctgggc ctgc
//
LOCUS       DASWIY010000288         7317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3500230, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000288 DASWIY010000000
VERSION     DASWIY010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3500230"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VGA59_10550"
     rRNA            complement(<1..1154)
                     /locus_tag="VGA59_10550"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1786..3675
                     /locus_tag="VGA59_10555"
     CDS             1786..3675
                     /locus_tag="VGA59_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9720161.1"
                     /translation="MALITLLNAQLAFGHVPLLDHTDFSLEAGERVGLIGRNGAGKSS
                     LLKILAGLERPDDGTLQLQQGLRIAYVAQEPVLDAGTTVFAATLAGVADVIALIDQYT
                     HGHGDLDALQGAIEAKDGWNWEQRVEETLQRLHLDKNAVIGTLSGGTKKRVALGQALV
                     RRPDVLLLDEPTNHLDLDSIEWLENLLIDFKGSVVIVTHDRVFLDRVATRIVELDRGR
                     LLSYPGNFTQYQLQKEAQLAQEAVISAKADKLLAQEEVWIRKGVEARRTRAQGRVTRL
                     EKLRARRAARREVLGSVKLDVASGLPSGKIVAELKRVGKSFGAKTIVRDFTATILRGD
                     KVGVLGPNGAGKTTLLKLILGELAPGSGTVRQGANLQVAYFDQMRDALDLDATLEDFI
                     SPGSEWIEIGNQRKHVKSYLGDFLFSPARAGSPVRSLSGGERNRLLLARLFARPANVL
                     VLDEPTNDLDIDTLELLEDLLQNYDGTVFLVSHDRAFLDNVVTSTIVFEGNGLWREYE
                     GGVEDWLTQSRRAQALASAAAPAAEARTAAQPAAAAKPVHSAGSATPRKKLSYREQRE
                     LDALPGLIDALEQEQKAIHDELADGRLYLRDVGRAQQLTERIAQIEDDLMAALERWEA
                     LGTGL"
     gene            3820..5460
                     /locus_tag="VGA59_10560"
     CDS             3820..5460
                     /locus_tag="VGA59_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX9720162.1"
                     /translation="MLRCLVTLILSTWAAGCASLPANVERKASSAFDAPEQTVLGRLV
                     QERKAQAGARSDSAFRLLDSVGAAFSSRLALVESAQRSLDLQYYAIHADASTEILLQR
                     LRDAARRGVRVRILLDDFNIVGKDAQVLRLAFEPNVEIRLFNPLPGSRASLIGRLFTS
                     LHDMPRIQKRMHNKLFIADNAMGITGGRNLGDAYFGTDDKSNFVDLDVLAVGRVVRDM
                     SASFDRYWNNELAYPMQSLVSAGDLEKLRKEPAPAARDPSQAPPKPLPSMPAPTLTST
                     ILPGVTATAVVGPEPVPMDLRTIALAWAPAAVMVDKPGKIGPGDDEVDAGDTLVDGLL
                     QLMGEARQDVLIVSSYFVPGAPMMQAFARMRAKGVRIRVLTNSLASTDAPAAHAGYAR
                     YRKDLLALGVELYELRADQEGTLGGMGSTAGFGSGRAPGGSKSGASRASLHTKAVILD
                     QRLAVIGSMNLDLRSQIQNSEVALLIRSAAVSREAARQVEATIAQGAYRLELDKDRLV
                     WRAPPGAPFKDARSEPDASTKLRLLVRLIAPFAPDEML"
     gene            complement(5601..7058)
                     /locus_tag="VGA59_10565"
     CDS             complement(5601..7058)
                     /locus_tag="VGA59_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX9720163.1"
                     /translation="MLTRTSSQSLTEQLSTRFAQRIRDRLLAPGARLPSVRQCAQQQG
                     VSPSTVVAAYDQLLAQGMVEARKNRGFFVRESRTEPAPAAQRVPAKTSVSAWTTATGF
                     GTARGDAPIDATALIRGMFHKVSNKPQPGMGVFPPEWLESTFMPAAVRKVTSTRALQE
                     FSLQYGEPKGDSGLRRALSKKLATLSVHAAPENIITTVGATHALDIVSRTLLRHGDHV
                     MVEEPGWAVEFARLTALGMRLLPVPRGPDGPQLDVMARYCEVHQPKLYVSVSVLHNPT
                     GYCLSPGSAHRVLQLANQHDFHIIEDDTYSHIAPEYATRLCALDGLQRTIHVSGFAKI
                     LAPNWRVGFLAAHSSLIERLLDTKLLATLTTPALLEKALALCMEQGQLRRHAERIKTR
                     LDAARSRSVKLALHAGCTFAAEPAGLFGWVDTGVDTDALAQRMLDEGYLLAPGALFHA
                     QRKPSTLMRINFATTQDAVFWKKYAEVTRKMSSRA"
     gene            7127..>7317
                     /locus_tag="VGA59_10570"
     CDS             7127..>7317
                     /locus_tag="VGA59_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720164.1"
                     /translation="MCTGSKDYIVLYGKVKAHFIQEREAAAALAGIARCPGSAGRMKP
                     DEIKGLWLGLAGVTIFALT"
BASE COUNT         1262 a         2432 c         2274 g         1349 t
ORIGIN      
        1 gcagtctcat tagagtgccc aactaaatgt agcaactaat gacaagggtt gcgctcgttg
       61 cgggacttaa cccaacatct cacgacacga gctgacgaca gccatgcagc acctgtgtta
      121 cggctccctt tcgggcaccc ctccatctct ggagggttcc gtacatgtca aaggtgggta
      181 aggtttttcg cgttgcatcg aattaaacca catcatccac cgcttgtgcg ggtccccgtc
      241 aattcctttg agttttaacc ttgcggccgt actccccagg cggtcaactt cacgcgttag
      301 cttcgttact gagtcagtga agacccaaca accagttgac atcgtttagg gcgtggacta
      361 ccagggtatc taatcctgtt tgctccccac gctttcgtgc atgagcgtca gtgcaggccc
      421 aggggattgc cttcgccatc ggtgttcctc cgcatatcta cgcatttcac tgctacacgc
      481 ggaattccat ccccctctgc cgcactctag ccgtgcagtc acaaatgcag ttcccaggtt
      541 gagcccgggg atttcacaac tgtcttacat caccgcctgc gcacgcttta cgcccagtaa
      601 ttccgattaa cgcttgcacc ctacgtatta ccgcggctgc tggcacgtag ttagccggtg
      661 cttattctta cggtaccgtc atgacccctc tgtattagag aagggctttt cgttccgtac
      721 aaaagcagtt tacaacccga aggccttcat cctgcacgcg gaatggctgg atcaggcttg
      781 cgcccattgt ccaaaattcc ccactgctgc ctcccgtagg agtctgggcc gtgtctcagt
      841 cccagtgtgg ctggtcgtcc tctcagacca gctacagatc gacggcttgg tgagccttta
      901 ccccaccaac tacctaatct gccatcggcc gctccaatcg cgcgaggtct tgcgatcccc
      961 cgctttcatc cgtagatcgt atgcggtatt agctacgctt tcgcgtagtt atcccccacg
     1021 actgggcacg ttccgatgta ttactcaccc gttcgccact cgccaccagg gttgcccccg
     1081 tgctgccgtt cgacttgcat gtgtaaagca ttccgccagc gttcaatctg agccaggatc
     1141 aaactcttca gttcgatctt gatttgttgc cccttgcggg gccactcatt tggaatcgac
     1201 aaagaacttc atctctgaaa tttcttgtct atcttatgag cgtttaaagg tcttgcgacc
     1261 tagttccgaa gaacttggca atcgccttca aacgcccacg cttatcggct gtatgttgtt
     1321 aacgaacctt gtcggcatcc actcgacttt gcgtcttgct ttgcctgctt tgctgcgatc
     1381 agcgaagcct tgaattatcg caggttttgg caagtcctgt caacttgaag gtttccaccg
     1441 gcttgccctt gctgcttcgc gatgctcttc gcttcgcgct gcctggcact tcccgcagag
     1501 ccttgaattc tataccaggt tttggtccct ggtcaaaatc tcggcccttc tttcccccgc
     1561 cttgccgggt tccgcgtcct gcggctccct gctcaacaga gccgtaaact atagcacgga
     1621 aacgcccgca tgccagccat gaacgcgctg tttcacctcg gctcgacagg agcttccgca
     1681 gcctggggga ctcccgggtg ctgcgcggaa cggaacgttc cctgccgaac cgccgagttg
     1741 ccagacgagt cagctgcgcg gcggggcggg ataatcgccg ccttcatggc acttattacc
     1801 ctgctcaatg cacagctggc cttcggccac gtgccactgc tggaccatac cgacttctcg
     1861 ctcgaggccg gcgagcgcgt gggcctgatc gggcggaatg gcgccggcaa atcctcactg
     1921 ctcaagatcc tggcggggct ggaaagaccc gacgacggca ccctgcaatt gcagcaaggc
     1981 ctgcgcatcg cctacgtagc acaggagccg gtgctcgacg ccggcaccac cgtattcgca
     2041 gccaccctgg ccggcgtggc cgacgtcatc gcgctgatag accagtacac ccatggccac
     2101 ggcgacctgg acgcgctgca gggcgcgatc gaggccaagg acggctggaa ctgggagcag
     2161 cgggtcgagg agacgctgca gcgactgcac ctggacaaga acgcggtgat cggcacgctt
     2221 tccggcggca ccaaaaaacg cgtggccctg ggccaagccc tggtacgccg acccgacgtg
     2281 ctgctgctcg acgagccgac caaccacctg gaccttgatt ccatcgagtg gctggaaaac
     2341 ctgctgatcg acttcaaggg cagtgtcgtc atcgtcacgc acgaccgcgt tttcctcgat
     2401 cgtgtggcga cccgcatcgt cgagctcgac cgcggccggc tgctctccta tcccggcaac
     2461 ttcacgcaat accagctgca aaaggaagct cagctggcac aggaagccgt gatcagcgcc
     2521 aaagccgaca agctgctggc ccaggaagaa gtctggatac gcaagggtgt cgaggccagg
     2581 cggacccgag cccagggccg cgtcacccgg ctggaaaagc tgcgcgcgcg ccgcgcggcg
     2641 cggcgtgagg tgctgggcag cgtcaagctc gacgttgcct cgggcttgcc aagcggcaag
     2701 atcgtggccg agctcaagcg cgtcgggaaa tcgtttggcg ccaagacaat cgtgcgcgac
     2761 ttcaccgcca ccatcttgcg cggcgacaag gtcggcgtgc tcggacccaa tggcgccggc
     2821 aagaccacgc tgctcaagct catcctcggc gagctcgcgc cgggcagcgg cacggtgcgc
     2881 cagggcgcca acctgcaggt cgcctacttc gaccagatgc gcgacgcgct ggacctggac
     2941 gccacgctgg aggacttcat cagccccggc agcgagtgga tcgagatcgg caaccagcgc
     3001 aagcatgtca agagctacct cggcgacttc ctgttctccc cggcgcgggc gggctcgccg
     3061 gtacggtccc tttccggcgg cgagcgcaac cggctgctgc tggcgcgcct gttcgcacgg
     3121 ccggcaaacg tgctggtcct ggacgaaccc accaacgacc tggacatcga cacgctggaa
     3181 ctgctggaag acctgctgca gaactatgac ggcacggtgt ttctcgtcag ccacgaccgg
     3241 gcgttccttg acaacgtggt gaccagcacc atcgtgttcg agggcaacgg cctgtggcgc
     3301 gagtacgaag gcggcgtgga ggactggctc acccagtcgc gccgggccca ggccttggca
     3361 tcggcggccg cgccggccgc cgaagcgcgc acggcagcgc agccggcggc cgccgccaaa
     3421 ccggtgcact ccgccggcag tgcaacgccc cgcaagaagc tgagctaccg ggagcagcgc
     3481 gagctcgacg cgctgccggg cctcatcgac gcgctcgaac aggagcagaa ggcaatccac
     3541 gacgaattgg ccgacggccg cctctacttg cgcgacgtcg ggcgcgccca gcaactgacc
     3601 gagcgaatcg cgcagatcga ggacgacttg atggcggcgc tggagcggtg ggaggctctg
     3661 ggcaccggct tgtagccttt gtccggcagc aactagcgac tacagctgac ctggctcgcg
     3721 cccgcagcgc cagctgcggg ggtagagtgg catgtaccca tgcagtacct tccctccgcc
     3781 tcctcgtcgc gccatccgcc gggtcttcga tgggcggtca tgttgcgctg cctggtgacc
     3841 ctgattcttt cgacctgggc ggccggatgc gcgagcttgc cggccaacgt ggagcgcaaa
     3901 gcatcgagcg ccttcgacgc gccggagcag acggtgctgg gacggctcgt ccaggagcgc
     3961 aaggcgcagg ccggcgcgcg cagtgattcg gccttccgcc tcctggacag cgtcggtgct
     4021 gcgttttcca gccggctggc gctggtcgag tcggcccaac gcagcctgga cctgcagtat
     4081 tacgccattc acgccgacgc cagcacggaa atcctgctgc agcggctgcg cgacgccgcc
     4141 cgccgcggcg tgcgggtgcg catcctgctg gatgacttca acatcgtggg caaggacgcc
     4201 caggtgctgc ggctggcgtt cgagcccaac gtggagatcc ggctgttcaa tcccctgccg
     4261 ggttcccgcg catcgctgat tggccgcctt tttacctccc tgcacgacat gccacgcatt
     4321 caaaagcgca tgcacaacaa gctgttcatc gccgacaacg cgatgggcat tacgggcggg
     4381 cgcaacctgg gcgacgctta tttcggcacg gacgacaaga gcaatttcgt cgatctggat
     4441 gtgctcgccg tcggccgcgt ggtacgggac atgtcggcca gcttcgaccg ctactggaac
     4501 aacgagctgg cttacccgat gcagtcactc gtttcggccg gcgacctgga aaagctgcgc
     4561 aaagagcccg cacctgcggc tcgcgatcca tctcaggctc ccccgaagcc cttgcccagc
     4621 atgccggcgc caactctcac ctcgacgatc ctcccgggcg tcaccgcgac cgcggtggtc
     4681 ggacccgagc ccgttcccat ggacttgcgc accattgcgc tcgcgtgggc gccggccgcg
     4741 gtcatggtgg acaagccggg gaagatcgga cccggcgatg atgaagtcga cgcgggcgac
     4801 accctggtgg acggcctgct gcagctgatg ggcgaggcgc gccaggacgt tctcatcgtg
     4861 tcctcctatt tcgtgccggg tgcgccgatg atgcaggcgt tcgcccgcat gcgcgccaag
     4921 ggcgtgcgca tccgtgtgct gaccaactcg ctggcttcca ccgatgcccc ggccgctcat
     4981 gccggctatg cgcgctaccg caaggacctg ctcgcgctgg gggtggagct ctacgagctg
     5041 cgggccgacc aggaaggtac cctgggcggc atgggttcca ccgccggctt cggatccggc
     5101 cgcgccccgg gcggctccaa gagcggcgcg tcacgggcca gcctgcacac caaggccgtg
     5161 atcctggacc agcggctggc ggtgatcggc tcgatgaacc tcgacctgcg ctcccagatc
     5221 cagaacagcg aggtcgcact cctgatccga agtgccgccg tgtcgcgtga ggcggcgcgc
     5281 caggtggaag ccaccatcgc tcagggcgcg taccggctcg agctggacaa ggaccggctg
     5341 gtatggcgcg caccgcccgg cgcgcccttc aaggacgccc gtagcgaacc ggacgccagc
     5401 accaagctga ggctcctggt gaggctgatt gcgccgttcg ccccggacga gatgctgtaa
     5461 gggcagcgtc attgcggacc cggatcaggc ctgccccgga cttgatccgg ggtccggggc
     5521 aggcccgatc cgcaatcctg acttgggcct accatgtcat tgcggactcg atccgcaatc
     5581 catgactcct ggatcccggg tcaggcccgg gatgacatct ttcgcgtcac ctccgcatac
     5641 ttcttccaga acaccgcgtc ctgcgtggtc gcgaagttga tccgcatcag cgtggacggc
     5701 ttgcgctggg cgtggaacag tgcgccgggc gcgagcaggt agccttcgtc gagcatgcgc
     5761 tgggccagcg catcggtgtc cacaccggta tcgacccacc cgaacagccc cgccggttcc
     5821 gccgcgaagg tgcagcccgc gtgcagggcg agcttcacgc tgcggctgcg cgccgcatcg
     5881 agccgggtct tgatgcgttc ggcatggcgc cgcagctggc cctgctccat gcacagggcc
     5941 agcgcctttt ccagcaatgc cggcgtggtc agcgtggcca gcaacttggt atcgagcagg
     6001 cgctcgatca aggaggaatg cgccgccagg aagccgacgc gccagttcgg cgccaggatc
     6061 ttggcgaatc cgctgacgtg gatcgtccgc tgcagcccgt cgagcgcgca cagccgcgtc
     6121 gcatattcgg gggcgatgtg gctgtaggtg tcgtcctcga tgatgtggaa gtcgtgctgg
     6181 ttggccagct gcagcacgcg gtgcgcactg ccgggcgaca ggcagtagcc cgtcgggttg
     6241 tgcaacacgc tcacgctgac gtagagcttg ggctggtgga cttcgcaata gcgcgccatc
     6301 acatccagtt gcggcccgtc gggtccgcgc ggcacgggca gcaggcgcat gcccagggcc
     6361 gtgagccggg cgaattcgac cgcccacccc ggctcctcga ccatcacgtg gtcaccgtgc
     6421 cgcagcagtg tgcggctgac gatgtccagg gcgtgggtgg cgcccacggt ggtgatgatg
     6481 ttttccggcg cggcgtgcac ggacaaggtc gccagtttct tggaaagcgc ccgccgcagc
     6541 ccgctgtcgc ccttgggctc gccgtattgc agcgagaact cctgcagcgc ccgggtgctg
     6601 gtgaccttgc gcaccgccgc cggcatgaag gtcgattcca gccattccgg cggaaacacg
     6661 cccatgccgg gttgcggctt gttgctgacc ttgtggaaca tgccgcggat cagcgcggtg
     6721 gcatcgatcg gggcgtcacc gcgtgccgtg ccgaagccgg tggccgtggt ccaggccgaa
     6781 accgacgtct ttgcgggaac ccgctgcgca gccggcgcgg gctcggtgcg gctttcgcgc
     6841 acgaagaagc cccggttctt gcgcgcctcc accatgccct gcgccagcaa ctggtcgtag
     6901 gcggccacca ccgtcgacgg actcacgccc tgctgctgcg cgcattgacg caccgagggc
     6961 agtcgcgccc cgggcgccag caggcgatcg cgaatacgct gcgcaaagcg cgtggacagc
     7021 tgctcggtga gcgattgcga tgaagtcctg gtcagcatgg gcgactcctc gtgtattgcc
     7081 agctcgggca atacagaacc cgaactgcgc tgcctgactg tattgcttgt gtactggtag
     7141 caaagactac attgttctgt atggaaaggt caaggcccac ttcatccagg agcgcgaagc
     7201 agccgctgcc cttgccggca tcgcgcgatg cccggggagt gccggccgta tgaagcccga
     7261 tgaaatcaag gggctctggc tcgggctggc gggtgtcacg atcttcgcgc tcacgtt
//
LOCUS       DASWIY010000289         7189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_926643, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000289 DASWIY010000000
VERSION     DASWIY010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926643"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="VGA59_10575"
     CDS             complement(<1..1687)
                     /locus_tag="VGA59_10575"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX9720165.1"
                     /translation="MSDTSPQQPGLNSLSKSFEPAAIEAKWGPLWEERGYARAGYRGT
                     GEPREGEPAFSIQLPPPNVTGTLHMGHAFNQTIMDSLARYHRMRGFNTLWVPGTDHAG
                     IATQIVVERQLQDQGQSRHDLGRKNFVAKVWEWKEKSGNIITTQMRRMGDSVDWKHEY
                     FTMDDNLSKVVTETFVQLYEQGLIYRGKRLVNWDPELKTAVSDLEVESEEEEGFLWYI
                     RYPLVDPDGSLAAAGTQALTVATTRPETMLGDVAVMVHPDDERYRHLVGKRVLLPLCD
                     RELPVIADEYVDKEFGTGVVKVTPAHDANDYAVGQRHKLPIIGVLTLDAKINDNAPAK
                     YRGLDRFVARKQVVADLKALDLLAETKKHKLTVPRCGRTGQIVEPMLTDQWFVAMSKV
                     SAKDPTGKSIAQKAIDAVESGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPA
                     WYDEDGKVYVARDEAHAQAQAPGKALRRDEDVLDTWYSSALVPFSTLGWPDKTREMDL
                     YLPSSVLVTGYEIIFFWVARMIMMTTHFTGQVPFRDVYIHGMVRDSEGKKMSKS"
     gene            complement(1759..1947)
                     /locus_tag="VGA59_10580"
     CDS             complement(1759..1947)
                     /locus_tag="VGA59_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HEX9720166.1"
                     /translation="MKATLNLQDADGFYELLLDAHEGLSPEQSQLLNARLILLLANQV
                     GDAKVLKECIEAAKTLPK"
     gene            complement(1944..3659)
                     /locus_tag="VGA59_10585"
     CDS             complement(1944..3659)
                     /locus_tag="VGA59_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9720167.1"
                     /translation="MAASGTQTSTSSGYEFTQGGAYVLPEYPFVEPPEIRKGVVSHHP
                     IVIVGGGLAGLTMACSLARYGVPAVLLDEDNTVGAKGASSRGICYTQKSLEIFQRLGV
                     YDRIAAKGIQWSVGRTFAGSDEVYSFDLRQQSAYNLSSQPPFINIQQFYIEGFLVERI
                     RELGRVDVRWCSRVIAFEQDNECTTLTVETPAGDYRIRADHVIDATGAHSPFRAWAGA
                     SMTSKKGDDRWCIADVRFSRHPPVERHTWIEAPFNDNRAVWQHLMGDNVWRIDYQMGL
                     HADADYVSREDVVRERLARQFGDDCEIEIVWVGPYTYRSECIDQMRHGRVFFMGDAAK
                     VVSPFGARGGNTGVADADNLAWKLAAVMKGQAAPALLDSYHEERHEAARENVLVTNRT
                     ARFLRPADGMERLFRDAALGLARQYVFARQLVNTGRMAIANPYTRSSVCDASGGLSVQ
                     NVSFQWPDGSEGTLNDLLEWAGGRLLLLLFGNASPRCYERLRALGETAPVRSVHVLGA
                     DEPALALEHVRDPKGHLQGACHVFGHAWALIRPDSYIAATGESVDTTLVHAVSKSIAA
                     EGEGA"
     gene            complement(3822..4442)
                     /locus_tag="VGA59_10590"
     CDS             complement(3822..4442)
                     /locus_tag="VGA59_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9720168.1"
                     /translation="MSAVPTDAGDLPAPPPIQQLHHYAYRARDAEETRHFYEDILGLP
                     LYHIIQSDYVPSTGEYCPYTHFFFRLQDGSFIAFFDLGDDQAAQPSPNTPLWVNHISF
                     RVDSQQALRDMKARLEAHGIEVLGVTDHHIFHSIYFFDPNGIRLELTAQLADEFQMLQ
                     ESKTAHARLAEWTARKDQWRKDRASGKAGEPLKPQQNDRPELVGRK"
     gene            4687..5109
                     /locus_tag="VGA59_10595"
     CDS             4687..5109
                     /locus_tag="VGA59_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX9720169.1"
                     /translation="MAELKGSKTEENLKAAFAGESQANRRYLYFANKADVEGQNDVAA
                     LFRSTAEGETGHAHGHLEWLEQCGDPATGLPIGPTRDNLKAAVAGETHEYTDMYPGMA
                     RTAREEGFDEVADWFETLAKAERSHANRYTRALNELVD"
     gene            5179..6531
                     /locus_tag="VGA59_10600"
     CDS             5179..6531
                     /locus_tag="VGA59_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX9720170.1"
                     /translation="MAPREGNLEAPTRHPVDWKNPEYYDEKPAFDELERIFHICHGCR
                     RCVSLCGAFPTLFDLVDESKTGEVDGVDRKDYRKVVDQCYLCDLCYMTKCPYVPPHEW
                     NVDFPHTMLRAKAIKFKQGGVSFGEKFLSSTDVHGQFAGIPIVVQVANAVNRTKPARA
                     AMEKILHVDRNAWMPALATRKFRSGAPKSADFAVKDGAKTPGKVAIFSTCYINYNEPG
                     IGHDLLKLLNHNEVPYVLVNKEKCCGMPKLELGDLESVNQAKEANIPVLVEYAKQGYA
                     ILTAVPSCTLMFKQELPLMYPGDADVQAVKDAMWDPFEYLMARKRDGLLKTDFSRPLG
                     KVSYHIPCHNRVQNIGKKTEEMLKMVPETFVQTNERCSGHAGTFGVKKPFHEVAMKIG
                     KPLFKRMSEHPQGGLPDYISSDCPLGGHHINQGFDRNSLGTPKLEHPLTLVRMAYGLS
                     "
     gene            6670..>7189
                     /locus_tag="VGA59_10605"
     CDS             6670..>7189
                     /locus_tag="VGA59_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEX9720171.1"
                     /translation="MQLTGKITRDSLMTLEAYSRYRKDHKADVIAHRKLRSVALGEHI
                     TMQFESELTIRYQIQEMLRLEKIFEEEGIEQEIQAYAPLMPDGSNWKATMMIEYPDVN
                     ERKRELGRLIGIEDRMFVEVEGHPRVYAIADEDLDRENEEKTSAVHFVRFEFTPPMVS
                     AIKAGAGVKLGCD"
BASE COUNT         1301 a         2267 c         2292 g         1329 t
ORIGIN      
        1 cggacttgct catcttcttg ccttcgctgt cgcgcaccat cccgtggatg tacacgtcgc
       61 gaaagggcac ctgcccggtg aagtgcgtcg tcatcatgat catccgggcg acccagaaga
      121 agatgatctc gtagccggtg accagcaccg acgacggcag gtacaagtcc atttccctgg
      181 tcttgtcggg ccagccgagc gtagagaacg gcaccagcgc cgaggagtac caggtgtcca
      241 ggacgtcctc gtcgcggcgc agcgccttgc ccggcgcctg cgcctgggca tgcgcttcgt
      301 cgcgcgcgac gtagaccttg ccgtcctcgt cgtaccacgc gggaatctgg tgtccccacc
      361 agagctggcg cgagatgcac cagtcctgga tgttgttcat ccactggttg tacgtgttga
      421 cccagttctc cgggatgaag cgcaccgcgc ccgattccac cgcatcgatc gccttttgcg
      481 cgatggactt gccggtcggg tccttggcgc tgaccttgct catcgcgacg aaccactggt
      541 cggtcagcat cggctcgacg atctggccgg tgcgtccgca gcgcggcacc gtcaacttgt
      601 gcttcttcgt ttcggcgagc agatccagcg ccttcaaatc cgccaccacc tgcttgcgcg
      661 cgacgaagcg gtccagcccg cggtacttcg ccggcgcgtt gtcgttgatc ttcgcgtcca
      721 gcgtcagcac gccgatgatc ggcagcttgt ggcgctggcc gaccgcgtag tcgttggcgt
      781 cgtgggcggg cgttaccttc accacgccgg tgccgaattc cttgtccacg tattcgtcgg
      841 cgatgacggg gagctcgcga tcgcaaaggg ggagcaaaac gcgcttgccc accaggtgac
      901 ggtagcgctc gtcgtccgga tgcaccatca cggcgacgtc gcccagcatg gtttcgggcc
      961 gggtggtggc cacggtgagt gcttgcgtcc cggcagccgc aaggctgccg tccggatcca
     1021 cgaggggata gcggatgtac cagagaaagc cctcttcctc ctcgctctcc acttcgaggt
     1081 cgctcacggc ggtcttgagt tcggggtccc agttcaccag gcgcttgccg cggtagatca
     1141 ggccctgttc gtacaactgc acgaaagttt cggtgaccac cttcgacagg ttgtcgtcca
     1201 tggtgaagta ttcgtgcttc cagtccacgc tgtcgcccat gcggcgcatc tgcgtggtga
     1261 tgatgttgcc cgacttctcc ttccactccc acaccttggc gacgaagttc ttgcggccca
     1321 gatcgtggcg cgactggccc tggtcctgca gctggcgttc caccacgatc tgcgtggcga
     1381 tgccggcgtg gtcggtgccc ggcacccaca gcgtgttgaa gccgcgcatg cggtggtacc
     1441 gcgccaggct gtccatgatg gtctggttga aggcatggcc catgtgcagc gtgcctgtca
     1501 cattgggtgg cggcagctgg atcgagaaag cgggctcgcc ctccctgggc tcgccggtgc
     1561 cgcggtagcc agcccgggcg tagccgcgtt cctcccacaa cggaccccat ttcgcctcga
     1621 tggcggcggg ctcgaaggac ttggacaggc tgttcaggcc gggttgctgc ggggacgtgt
     1681 cgctcataat ttaattgggg tcagattcca atttttaatt ggaatctgac cccaatttgt
     1741 gtgggggatg gagcgatttt acttgggcag cgtcttcgcg gcctcgatgc actccttgag
     1801 caccttggcg tcgccgacct ggttggccag cagcaggatc aggcgggcgt tgaggagctg
     1861 cgactgctcc ggcgacaggc cctcgtgcgc atcgagtagc agctcgtaaa aaccgtcggc
     1921 gtcctgcagg ttcagtgtcg ctttcatgcg ccttctcctt cggccgcgat cgacttggat
     1981 acagcatgga ccagcgtcgt gtcgacgctt tcaccggtgg ccgcgatgta actgtccggg
     2041 cggatcaggg cccaggcatg gccgaagacg tggcaggcgc cttgcaggtg gcctttcggg
     2101 tcgcgaacat gctcgagcgc cagggcgggc tcgtcggcgc ccagcacatg cacgctgcgc
     2161 accggtgccg tttcgcccag cgcccgcagc cgctcgtagc atcgtgggct ggcattgccg
     2221 aaaagaagca ggagcaggcg gccgccggcc cactccagca ggtcgttgag cgtgccttcc
     2281 gagccgtccg gccactggaa actcacgttc tgcaccgaca gcccaccgct cgcgtcgcac
     2341 acgctggagc gcgtgtaggg attggcgatg gccatgcggc cggtgttgac cagctgccgc
     2401 gcaaaaacgt actgccgcgc cagtcccaat gcggcgtcgc ggaacagcct ttccatgccg
     2461 tcggccggcc gcagaaaacg cgccgtgcgg ttggtcacca gcacgttctc ccgcgccgct
     2521 tcgtggcgct cctcgtggta gctgtccagc aaggcagggg ccgcctgacc tttcatcacg
     2581 gcggccagct tccaggccag gttgtcggca tcggcgacac cggtgttgcc gccacgcgcg
     2641 ccaaaggggc tgaccacctt ggccgcatcg cccatgaaga agacccggcc gtgccgcatc
     2701 tggtcgatgc attcgctgcg gtaggtgtaa ggtccgaccc acacgatctc gatctcgcag
     2761 tcgtcgccga actgccgcgc caggcgctcg cgcaccacgt cttcccggct gacgtagtcg
     2821 gcatcggcgt gcaaccccat ctggtagtcg atgcgccaga cgttgtcccc catcaggtgc
     2881 tgccagacgg cccggttgtc gttgaacggc gcctcgatcc atgtgtggcg ctccaccggc
     2941 gggtgcctgc tgaaccgaac gtccgcgatg caccagcggt cgtccccctt cttcgaggtc
     3001 atcgatgcac cggcccaggc ccggaacggg ctgtgcgcgc cggtggcgtc gatcacgtgg
     3061 tcggcgcgga tccggtagtc gcccgccggt gtttcgaccg tcagcgtcgt gcattcgttg
     3121 tcttgctcga aggcgatgac gcggctgcac cagcgcacgt ccacacggcc gagttcccgg
     3181 atgcgttcga ccaggaagcc ctcgatgtag aactgctgga tgttgatgaa gggcggctgg
     3241 ctggacaggt tgtaggcgct ttgctggcga aggtcgaatg aatagacctc gtcgctgcct
     3301 gcgaaggtgc ggcccacgct ccactggatg cccttggcgg cgatgcggtc gtagaccccc
     3361 agccgctgaa agatctccag cgatttctgt gtgtagcaga tgccgcgcga cgatgcgccc
     3421 ttggcaccga cggtgttgtc ctcgtccagc agcacagccg gcacgccgta gcgcgccagc
     3481 gaacaggcca tcgtcaagcc ggccagtccg cccccgacga tgacgatggg atggtggctg
     3541 accacgccct tgcggatttc aggcggctcc acgaacgggt actcaggcag cacgtaggcg
     3601 ccgccctggg tgaactcgta accgctgctg gttgatgtct gcgtgccgct tgccgccatg
     3661 cgctgtctcc tcgttctgtc gttgcgtttg agccgctgct cctggatccc gggtcaagcc
     3721 cgggatgaca gcccaattgc cctgtcattg cgggcttgac ccgcaatcca ttgcgtccca
     3781 tcaagcgcgg catgaccaac ctgttccagg tgatcaccat cttattttct acccacgagc
     3841 tcgggccggt cattttgctg cggtttgagt ggttcgccgg ccttgcccga ggcgcggtcc
     3901 ttgcgccatt ggtccttgcg cgcggtccac tcggccagcc gggcatgggc agtcttgctt
     3961 tcctgcagca tctggaactc gtcggccagc tgggccgtca actccagccg gataccgttg
     4021 gggtcgaaga aatagatgct gtggaagatg tggtggtcgg tgacgcccaa cacctcgatg
     4081 ccatgggctt ccagccgggc cttcatgtcg cgcagcgcct gctgcgagtc gacgcgaaac
     4141 gagatgtggt tgacccacag cggcgtgttg ggcgagggct gggccgcctg gtcgtcgccg
     4201 aggtcgaaga acgcgatgaa agaaccgtcc tgcagccgga agaagaaatg ggtgtaaggg
     4261 cagtattcgc cggtgctggg gacgtagtcg ctctggatga tgtggtacag cggcaggccc
     4321 aggatgtcct cgtagaaatg ccgggtttcc tcggcgtccc tggcccggta ggcgtagtgg
     4381 tgcaactgct ggatgggcgg cggcgcgggc aggtcgccgg catctgtcgg tactgcggac
     4441 atgggctctc cttgtttact aatgcgtaac gcgtttacta taccgtaatc gctgcggcgg
     4501 taaccctggg ttgggttttg gggtatattc acgttggcta tcgattgata acatagatag
     4561 ttaacgttta tcgactgcat tgacgcagcc aattagacgg ctacccgcaa caggactaat
     4621 ctccaaaagg actttgaccc tgttcaaggc gaccggacgt ttcatttcaa cctataggag
     4681 atttccatgg cagaactcaa aggctcgaag acggaagaga acctgaaggc cgcctttgcc
     4741 ggcgaatccc aggccaatcg ccgctacctg tacttcgcga acaaggccga cgtggaaggc
     4801 cagaacgacg tggccgcgct gtttcgctcc accgcggaag gcgagaccgg ccatgcccac
     4861 ggccacctgg aatggctcga gcagtgcggc gaccccgcca ccggcctgcc gatcggcccc
     4921 acccgcgaca acctgaaggc cgcggtagcc ggagagaccc acgagtacac cgacatgtac
     4981 cccgggatgg ccaggacggc gcgcgaggaa ggtttcgacg aagttgccga ctggttcgag
     5041 acgctggcca aggcggagcg atcgcacgcc aaccgctaca ccagggcgct gaacgagctg
     5101 gtggactaag agaaattcct ctccctttcg gagagaaaat tctttccctc tcccacaggg
     5161 agagggagaa agaaatcaat ggcaccccgc gaaggcaatc tcgaagcgcc cacccggcat
     5221 cccgtcgact ggaagaaccc cgagtactac gacgagaagc ccgccttcga cgagctcgag
     5281 cgcatcttcc acatctgcca cggctgccgc cgctgcgtca gcctgtgcgg tgcctttccc
     5341 acgctgttcg acctggtcga cgagagcaag accggcgaag tggacggcgt ggaccggaag
     5401 gactaccgga aggtggtcga ccagtgttac ctgtgcgacc tgtgctacat gaccaagtgc
     5461 ccatacgtgc cgccgcatga gtggaacgtc gactttccgc acaccatgct gcgcgccaag
     5521 gcgatcaagt tcaagcaggg cggggtgtcc ttcggcgaga aattcctctc gtcgaccgac
     5581 gtgcacggcc agttcgccgg catcccgatc gtggtgcagg tcgcgaacgc cgtcaatcgg
     5641 accaagccgg cgcgcgccgc gatggaaaag atcctgcacg tggaccgcaa cgcctggatg
     5701 ccggcgctgg ccactcgcaa gttccgctcc ggcgcgccca agtcggcgga cttcgccgtg
     5761 aaggacggcg ccaagacgcc gggcaaggtc gcgatcttct cgacctgcta catcaactac
     5821 aacgagccgg gcatcggcca tgacctgctc aagctgttga accacaacga agtgccttac
     5881 gtgctggtga acaaggaaaa atgctgcggc atgcccaagc tggagctggg cgacctggag
     5941 tcggtgaacc aggccaagga agcgaacatc ccggtgctgg tggagtatgc gaagcagggc
     6001 tacgccatcc tcaccgccgt gcccagctgc acgctgatgt tcaagcagga actgccgctg
     6061 atgtatcccg gcgacgccga cgtgcaggcc gtcaaggacg cgatgtggga cccgttcgag
     6121 tacctgatgg cccgcaagcg cgacggcttg ctcaagaccg atttcagccg gccgctgggc
     6181 aaggtcagct accacattcc ctgtcacaac cgggtgcaga acatcggcaa gaagaccgaa
     6241 gagatgctga agatggtgcc cgagaccttt gtgcagacga atgagcgttg ctcgggccac
     6301 gccggcacgt tcggcgtgaa gaagcccttc catgaggtcg ccatgaaaat cggcaagccg
     6361 ctgttcaagc gtatgtctga acatcctcag ggcggtctgc ccgactacat cagctccgac
     6421 tgcccgctgg gcggccacca catcaaccag gggttcgacc gcaactcgct gggcacgccg
     6481 aaactggagc acccgctgac gctggtcaga atggcctatg ggctaagcta gcctctcatg
     6541 cgggcttgac ccgcaatcca tgcgggcgtg gcatgcgcgc atggatgccg gatcaagtcc
     6601 ggcatgacag cccagggggt ccggcatggc tgccaaggag cctgggacga caacgtaggg
     6661 agctggagaa tgcaactcac cggaaaaatc acccgcgaca gcctgatgac gctggaggcg
     6721 tatagcaggt accgcaagga ccacaaggcg gatgtcatcg cgcaccgcaa gctgcgcagc
     6781 gtcgccctgg gcgagcacat cacgatgcag ttcgagagcg agctgacgat ccgctaccag
     6841 atccaggaga tgctgcggct cgaaaagatt ttcgaggaag aaggcataga gcaggaaatc
     6901 caggcctatg ccccgctgat gcccgatggc agcaactgga aggcgacaat gatgatcgag
     6961 taccccgacg tcaacgaacg caagcgcgaa ctgggccgcc tgatcggcat cgaggaccgc
     7021 atgttcgtcg aggtggaggg ccacccgcgc gtgtacgcca tcgccgacga ggatctggat
     7081 cgcgaaaacg aggagaagac gtcggcggtg catttcgtgc gcttcgaatt cacgccgccg
     7141 atggtgtcgg cgatcaaggc cggcgctggc gtgaagctcg gctgcgatc
//
LOCUS       DASWIY010000290         3064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3502441, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000290 DASWIY010000000
VERSION     DASWIY010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3502441"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VGA59_10610"
     CDS             <1..679
                     /locus_tag="VGA59_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX9720172.1"
                     /translation="AESLGARYKGRASGTFGKIGVYSFNGNKIITTSGGGMLVADDPA
                     LMERARKLATQAREPAAHYEHVEVGFNYRMSNVLAGIGRGQLRVLEQRVAQRRKVFDK
                     YREALADQPQLEWMPEPQGYQSTRWLTSFSLRGPNAELRRDLVLRSLERHSIEARPVW
                     KPMHRQPLFRGAPYFPHGGEDVSARLFESGICLPSGSNLTPDQQGRVIEQLRRALALA
                     EERRAAA"
     gene            666..2471
                     /locus_tag="VGA59_10615"
     CDS             666..2471
                     /locus_tag="VGA59_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX9720173.1"
                     /translation="MRLPRDTLVLLAVDLALVLLAGWIAFWLRFNFDIPSEFDALALR
                     ASPWCVAGYAAGLTLARVYRQVWSYIGLPELRQLAVGIMLGALLTAAAVLMLRMPAFP
                     RSVLLLQPLIALLLLGAARASWRTVVERRTLGQGDGRRLIIVGSLQDASDALRALKGS
                     HQWRPVGIVSPAAAEVGRSMQNVRVLGQTAAISDICAAAQAEAALVASPPGSAERREA
                     LMSAAGSRLTLLTMPRPDEWLKTESAGPRKIELEDLLGRTAVQLDEAGLAELFSGQTV
                     LVTGAGGSIGSELCRQVARFGVGRLVCLDVSEYAIYQLEQELREAHPQMHGCYYTANV
                     REVDRLRAIALKHRPAVMFHAAAYKHVPLMEELNEIEALRTNVLGTLHAARVAGECGA
                     RRFVMISTDKAVNPTNVMGSSKRLAELMVQGVAAGNGGTQYVSVRFGNVLGSSGSVVP
                     LFAAQIARGGPVTVTHPEIVRYFMTIPEAAQLVLQAGLMGRSGQIFVLDMGEPMKIVE
                     LARMLIRLSGKTEQEIPISFTGLRPGEKLFEELLADDETTEPTPHPKLRVAKTAGQLP
                     DIEAVMRWICEAGPAPAGDDLRRWLRSQVPEYSSR"
     gene            complement(2536..>3064)
                     /locus_tag="VGA59_10620"
     CDS             complement(2536..>3064)
                     /locus_tag="VGA59_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX9720174.1"
                     /translation="DMIGRCHKAGAPFGVVLLTQGNEVRQPGSTEAFSHVGTLATIGE
                     LETPRPGLMMIRASGTQRFRITASDQLRHGLWIADVERLAPDMTVPIPDDLKNTAQAL
                     GKLIHSLQLKTTAPGQMPLQGPWRLDDCGWVANRWCELLPLPMPLKQRLMELDNPLVR
                     LELVSDVLARTGIAG"
BASE COUNT          490 a          991 c         1068 g          515 t
ORIGIN      
        1 ggccgaatcg cttggggccc gatacaaggg ccgtgccagc ggcacctttg gaaagatagg
       61 cgtgtattcc tttaacggca acaagatcat cacgacctcc ggcggcggaa tgctggtggc
      121 cgacgatccg gcgctcatgg aacgcgcgcg caagcttgcc acccaggcgc gcgagccggc
      181 cgcgcattat gaacacgtgg aggtgggctt caactaccgc atgagcaatg tgctggccgg
      241 catcggccgt ggccagctgc gcgtgctgga gcagcgagtg gcccagcggc ggaaggtttt
      301 cgacaagtac cgtgaggcat tggccgacca gccgcaactg gagtggatgc cggagccgca
      361 aggctaccag tctacgcgct ggctcaccag tttcagcctg cgaggcccga atgccgaact
      421 gcggcgcgac ctggtgctca ggtcgctgga gcgccattcg atcgaagccc ggccggtatg
      481 gaagccgatg catcggcaac cgctgttccg cggagcgccg tattttccgc acggcggcga
      541 agatgtgtcg gcccgccttt tcgaatcggg aatctgcctg ccttcaggat cgaacctcac
      601 ccccgaccag cagggccggg tgattgaaca gctgcgccgg gctttggcgc ttgcggagga
      661 gcggcgtgcg gctgcctaga gacacgctgg tactgctggc tgtcgacctg gcactggtgc
      721 tgctggcagg gtggatcgcg ttctggcttc gtttcaattt cgacattccc tctgaattcg
      781 acgccctggc gttgagggcc agcccatggt gcgtagccgg ctacgcggcc ggcctgactt
      841 tggcaagggt ttaccgccag gtctggagct acatcggcct gccggaactc aggcagctgg
      901 cagtgggcat catgctcggc gcattgttga cggcggccgc ggtactgatg ctgcgcatgc
      961 cggcctttcc gcgctcggtg ctgctgctgc agccgctgat cgcgctgctc ctgctaggcg
     1021 ccgcgcgggc gagctggcgc acagttgtcg aacgccgcac gctggggcag ggagacggcc
     1081 gccgcctgat catcgtgggc tcgctgcagg acgcgtccga tgcgctgcgg gcgctcaagg
     1141 gctcgcatca atggcgcccg gtaggcatcg tctcgcccgc cgccgccgag gtaggccgat
     1201 cgatgcagaa cgtgcgagtg ctgggccaga ccgccgccat ctcggacatt tgcgccgctg
     1261 cccaggccga agccgccctg gtggcgagcc cgccgggctc ggccgagagg cgcgaggcgc
     1321 tcatgagcgc agccgggtcg cggctgacgc tcctcaccat gccccgcccc gacgaatggc
     1381 tcaagaccga atcggcgggg ccgcgcaaga tcgagctgga ggacctgctc gggcgcacgg
     1441 cggtacagct cgacgaagcc gggctggcgg agctgttctc tggccagact gtcctggtca
     1501 cgggggcggg cggctccatc ggctccgagc tgtgccgcca ggtcgcgcgc ttcggcgtgg
     1561 gacgccttgt ctgcctcgac gtgtcggagt acgcgatcta ccagctcgaa caggagctgc
     1621 gtgaggcgca cccgcagatg cacggttgct attacacggc caacgtgcgt gaagtcgacc
     1681 gcctgcgcgc gatcgcactc aagcatcgac cggcggtgat gtttcacgcc gcggcgtaca
     1741 agcacgtgcc gctaatggag gaactcaacg agatcgaagc attgcgcacc aatgtgctgg
     1801 gaaccttgca cgcggcgcgc gtggccggcg agtgcggcgc ccggcggttc gtgatgatct
     1861 ccacggacaa ggccgtgaac ccgaccaatg tgatgggctc gagcaaacgg ctggccgagc
     1921 tcatggtcca aggcgtggcg gccggcaacg gcgggaccca gtacgtctcg gtgcgcttcg
     1981 gcaacgtgtt gggatcgagc ggctcggtcg tgcccctgtt cgccgcgcag atcgcgcgcg
     2041 gcggcccggt caccgtgacc catcccgaga tcgtgcgcta cttcatgacg atcccggagg
     2101 cggcccagct ggtgctgcag gcggggctca tggggcgctc cggccagatc ttcgtcctgg
     2161 acatgggcga accgatgaag atcgtcgaac tcgcgcgcat gctgatccgc ctgtccggca
     2221 agaccgaaca ggaaattccc atcagtttca caggcttgcg gccgggcgaa aagctgtttg
     2281 aggagctgct ggccgacgac gagaccaccg agcccacgcc gcaccccaag ctgcgtgtgg
     2341 ccaagaccgc gggccagtta ccggacatcg aggcggtgat gcgctggatt tgcgaagccg
     2401 gaccggcgcc ggccggcgac gatctgcgcc ggtggctgcg cagccaggtg ccggaatact
     2461 cctcgcgctg aggatgcctg aaaccttgcg ggtcagatct ttagctccgt cctcaactga
     2521 ccgtgtgaac aggccctacc cggcgatacc ggtgcgggcc agcacgtcgc tcaccagctc
     2581 cagccgcacg agagggttgt ccaattccat caggcgttgc ttgagcggca tcggcagcgg
     2641 cagcagctcg caccagcggt tggccaccca cccgcaatcg tccagccgcc acgggccctg
     2701 caaaggcatc tgcccgggcg ccgtggtctt gagctgcagc gaatggatca gcttgcccag
     2761 ggcctgcgcc gtgttcttca ggtcgtcggg aatgggcacg gtcatgtcgg gcgcgagccg
     2821 ctcgacgtcc gcgatccaca ggccgtgccg gagctggtcg ctggcggtga tccggaagcg
     2881 ctgggtgccg ctggcgcgga tcatcatgag gccggggcgg ggcgtctcga gttcgccgat
     2941 ggttgcgagg gtgcccacgt gcgagaacgc ctcggtgctg cctggttgcc gcacttcatt
     3001 gccttgcgtc agcagcacga cgccgaacgg cgcgccggct ttgtggcagc ggcctatcat
     3061 gtcg
//
LOCUS       DASWIY010000291         5781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1683110, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000291 DASWIY010000000
VERSION     DASWIY010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683110"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VGA59_10625"
     CDS             complement(<1..480)
                     /locus_tag="VGA59_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720175.1"
                     /translation="MAALSMATDLAMGQPMEYAMASCVVSVRLGERAGLGPQDLRDAY
                     YEALLRYIGCNADVAWLSSIVGDEIALRREFAHVDSADHAASLALMLRSISAANAGAN
                     PLRATLAMVRGLSQLTLVHSSFFPGHCEVASRLAQRMGFAPSFVATVGQLYARWDGKG
                     "
     gene            complement(531..1040)
                     /locus_tag="VGA59_10630"
     CDS             complement(531..1040)
                     /locus_tag="VGA59_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HEX9720176.1"
                     /translation="MKRREFLIVSLTSAGGVAMADLSQVPSLDHSLRWLDSLEMTADV
                     KAIGAWPMGAVLEHLAQSIEMSMDGFPQTKGPLFQKTVGSAAFAFFKWRGRMSHGLAE
                     PIPGAPALSAGADWKPAALRLRQAIARFNGNSGPLKPHFAYGELSKAEFALAHSLHIA
                     NHRDEILVA"
     gene            1184..2203
                     /locus_tag="VGA59_10635"
     CDS             1184..2203
                     /locus_tag="VGA59_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720177.1"
                     /translation="MKAQLRNQVAALFLLAPAAVTFTALPSTVLAQSATPQVHSFQVA
                     SDDGLAPGSRLQFTVQGSPNARASIRIRGVRDRIPLAETSRGIYTGRYVIRRADRIDE
                     DSPIRAVLRRGNLSVAASYNFPPDMASVASSAPPPPALRIERFHMATVDRLEPGAELR
                     FTLEGLPGAVAFVDLPGIERNVRLRETRPGYYEGSYTIRRSDDLNIRGPVVATLRAGD
                     RFVTSNLAQPLVAAGPPNVPIRILSHSNNSQVDGGTTHVRGRTAPFASVEIKVDAVPP
                     VIGQFGVAQRVFSERMRADANGHFDFSFSSPFPVPGTRYDISMVASKGDVTNEARLVL
                     HQRQG"
     gene            complement(2302..2574)
                     /locus_tag="VGA59_10640"
     CDS             complement(2302..2574)
                     /locus_tag="VGA59_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720178.1"
                     /translation="MDPVPVSEVPISLGPNCYRLTTKGRACVDLVEAQGQTIGSLSRY
                     LHDVLMMCGSVAWFDQLRQFMPPRPLEQSLQSLLALGLIERLAPAA"
     gene            2963..3793
                     /locus_tag="VGA59_10645"
     CDS             2963..3793
                     /locus_tag="VGA59_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9720179.1"
                     /translation="MQCFTLTQQDHVAHLVLNRPEAMNTMHPAFWRELDQVLAQLHRE
                     GQSRALVISSTGKHFSAGMALETFGGEIAMDDRTPEGRAAILDLLTDMQATFTRLETL
                     RIPVIAAIQGGCIGGAVDMVTACCIRYATADAFFCIQEINIGMVADVGTLQRLPKLVP
                     LGVVKELAYTGRRLPAQKALAYGLVNELFETHEALVAAALQCAREIAAKPPVAIWGTK
                     QVIHFARDHSVDDSLKQMGWVQGAIWSNAHVREAVTAMKEKRGAEFPSLPPLRSFREL
                     "
     gene            complement(3797..4114)
                     /locus_tag="VGA59_10650"
     CDS             complement(3797..4114)
                     /locus_tag="VGA59_10650"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX9720180.1"
                     /translation="MTQGVERRQMFGYPCAFINGNMFAGLHEQRLIVRLPAQASSRPF
                     EVMGKVMKEYAAIEDALDCPPAQFRDWIARALAYARTLPPKKPKGKAKAGAKTTPRHP
                     ARR"
     gene            complement(4200..5045)
                     /gene="queF"
                     /locus_tag="VGA59_10655"
     CDS             complement(4200..5045)
                     /gene="queF"
                     /locus_tag="VGA59_10655"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913989.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEX9720181.1"
                     /translation="MNTPDQSQLGKPAPYADRYDASLLFPISRLPKRAELGLHGAVPF
                     FGADLWTAFELSWLTPRGKPQVAIAHVTVPCETPNIVESKSFKLYLNSFSGTAFASAE
                     EVRDRIRADVGEAVWRGAPTQSSVGLRLLLPEQFDREPVRELDGLSLDRLEIECTQYT
                     PAPELLTAAFGDQPVQEVLTSNLLKSNCPVTGQPDWGSIQIRYSGPQIDQGGLLRYLV
                     SFRNHNELHEQCVERIFMDLWRRCKPVKLVVYARYTRRGGLDINPFRTSHPMAPPPNV
                     RMARQ"
     gene            complement(5059..>5781)
                     /locus_tag="VGA59_10660"
     CDS             complement(5059..>5781)
                     /locus_tag="VGA59_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9720182.1"
                     /translation="VDNRDVGLSQHFDQLGVPNLLWESLKFRAGFAVRAPYSVHEMAA
                     DALGVLDALGIAQAHVVGVSMGAMIAQRVALAAPGRVLSLASIMGSSGARYLPGPKPQ
                     VLRVLLSRPKGGGEQAIVEHLVKLFRVIGSPGFPLDEAALRERLLASVRRSFHPAGTL
                     RQMTAVAADTRRADELAAIRTPTLVLHGREDPLVPFACGHDTARRIPGARFLGIEGMG
                     HDLPPGVVERLLEPLVPHLKPA"
BASE COUNT          982 a         1982 c         1878 g          939 t
ORIGIN      
        1 gcccttgcca tcccaacgcg catacagctg accgacggtc gccacgaacg acggcgcgaa
       61 acccatgcgc tgtgcgagtc gtgaagccac ctcgcaatgg ccaggaaaga aactgctgtg
      121 caccagcgtc aattgactca ggccccggac catcgccagg gtggcgcgca gcggattggc
      181 gcccgcgttg gcggcgctaa tggagcgcag catcaatgcg agcgacgccg catggtcggc
      241 gctgtccacg tgggcaaatt cgcgccgcag cgcgatctcg tctccgacga tcgacgacag
      301 ccaggcgacg tcggcattgc agccgatata acgcagcagc gcctcgtagt acgcgtctcg
      361 caggtcctgc gggcccaggc cggcacgctc gcccagccgg accgacacca cgcagctggc
      421 cattgcgtat tccatcggct ggcccatggc caggtccgtg gccatcgaca gcgccgccat
      481 cagctcagcc cgccgtactt ggccctcctc agcagtgaga gcggtgccgc tcacgcgaca
      541 aggatctcgt cgcggtggtt cgcgatatgc aagctatgcg ccagcgcgaa ctccgctttg
      601 ctcaattcgc cgtaggcgaa atgcggcttg agcggaccgc tgttgccgtt gaagcgcgcg
      661 atggcctggc gcaggcgcag cgcagcgggc ttccagtcgg ctccggcact caacgccgga
      721 gcgcccggta tcggctcggc caggccgtgg ctcatacggc cgcgccattt gaaaaaagcg
      781 aaggcggcac tgccgacggt cttctggaac agtggcccct ttgtctgcgg gaagccgtcc
      841 atgctcatct cgatgctctg cgccaggtgc tcaagcacgg cccccatcgg ccacgcaccg
      901 atcgccttga cgtcggccgt catttccagg ctgtcgagcc agcgcagcga gtgatccagc
      961 gatggcacct gggacagatc agccatggca acacctccgg cagatgtcag ggatacgatc
     1021 aggaactcgc gccgcttcat cgaccgcatg gtagcaagcc gcaggccgcg ccgcagtgcc
     1081 tgtgttggac ccgtcctacc gcgtcagccc ggctttgcct acaggccgcg cgccgattta
     1141 cccctacggt aaagagcaaa tccactattc caggggtgtc gatatgaaag cacagctacg
     1201 caatcaggtg gcggccctct tcctgctggc gccggccgcc gtaacgttca cggccctgcc
     1261 gtcgacagtg ctggcacagt ccgccacgcc gcaggtccat tcattccagg tggcaagcga
     1321 cgatgggctg gcaccgggct cgcgcctgca gttcacggtg cagggttcgc ccaacgcccg
     1381 cgccagcatc cgcatccgcg gcgtcaggga ccgcatcccg ctcgcggaaa cttcgcgcgg
     1441 catttacacc ggccgttatg tcatcaggcg ggccgaccgc atcgatgagg acagcccgat
     1501 ccgcgccgtc ctgcgccgcg gcaacctgag cgtggcggcc agctacaact tcccgccgga
     1561 catggccagc gtggcgtcgt cggcgccgcc gccgccggcg ctgcgcatcg agcgcttcca
     1621 catggccacg gtggaccggc tcgagccggg cgccgaactc aggttcacgc tcgagggctt
     1681 gcccggcgcc gtggcctttg tggatctgcc cggtatcgag cggaacgtcc gcctgcgcga
     1741 aacgcgtccc ggctactatg aaggcagcta cacgatcagg cgctcggacg acctcaacat
     1801 ccgcggcccg gtggtggcca ccttgcgggc gggcgaccgc ttcgtgactt cgaacctggc
     1861 ccagccgctg gtggcagcgg ggccgcccaa cgttcctatc cgcatcctga gccattccaa
     1921 caacagccag gtggatggcg ggacaaccca cgtgcgcggg cgcacggcac cgttcgcctc
     1981 ggtcgagatc aaggtcgacg ccgtaccgcc ggtcatcggc cagttcggcg tggcgcagcg
     2041 ggtcttctcg gaacgaatgc gggcggacgc caacggccac ttcgacttca gcttcagctc
     2101 gcccttcccg gtgcccggca cccggtatga catttccatg gtggcgagca agggcgatgt
     2161 gaccaacgag gcccggctgg tgctgcacca gcgccagggc tgacccagcg gctgtccggc
     2221 cgccgtcgcc gccgttgggc gggaaaggca atccgcgggg aaaccgtgga gcgcgccgcc
     2281 gtgcccggcc cggtagcccg gctaggcggc cggcgcgagc cgctcgatca gtccaagcgc
     2341 caacagggat tgcagcgatt gctcgagggg ccggggcggc atgaactgcc gcaactgatc
     2401 gaaccacgca accgagccgc acatcatcag cacatcgtgc aggtagcggg acagcgatcc
     2461 tatggtctgt ccctgggcct cgaccaggtc cacgcaggcg cggcctttcg tggtgaggcg
     2521 atagcagttc ggaccgaggc tgatggggac ttcagaaaca ggaaccgggt ccatttcatg
     2581 cctccttcaa tgaacatctt tccagtttca ctgtggccga cgtctctgac agccaactga
     2641 cgcgcggggt cggtatttac cagcaccgaa actgaatcgc acctgacatc cggctggcag
     2701 atagcctgtg ctcttgttac gatgctcccg aatctatagc agcggcaact tgctcctaac
     2761 ttgcagcaga actgacccga aacttacagc gcgcgtcgcc aggagatcgt gatgaagctc
     2821 gccgaactgc aacgcatcag gcaatggcac gtcgcccatc gcgccgacca tcctgtcgag
     2881 taccactcct gtcccccatt tgccacgcgc tcgtgtcgcg cggcattaca ttggcgatcc
     2941 agcctggtca aataggatcg ccatgcaatg cttcacgctc acccagcagg accacgtcgc
     3001 ccatctcgtg ctcaatcgcc ccgaggcgat gaacaccatg cacccggcgt tctggcgcga
     3061 attggatcag gtcctcgcgc aactccatcg cgagggacag tcacgggccc tggtgatttc
     3121 ctcgaccggc aagcatttca gcgccggcat ggcgctggaa actttcggcg gcgagatcgc
     3181 gatggatgac cgcacacccg aggggcgcgc cgcgatcctg gacctgctga ccgacatgca
     3241 ggccaccttc acccggctgg aaacgctgcg catccccgtc attgccgcga tccagggcgg
     3301 ctgcataggc ggcgcggtgg acatggtgac cgcctgctgc atccgttacg cgacggccga
     3361 cgcgttcttc tgtatccagg aaatcaacat cggaatggtg gccgacgtcg gcacgctgca
     3421 gcggctgccc aagctggtgc cgctgggcgt tgtgaaggaa ctggcgtaca ccggccgccg
     3481 gctgccggcc caaaaggcgc tggcgtatgg tctggtgaac gaactgttcg agacgcacga
     3541 ggccctggtc gccgcggcgc tgcagtgcgc acgcgagatc gccgccaagc cgccggtggc
     3601 gatctggggc accaagcagg tcattcactt cgcgcgcgac cattcggtgg acgattctct
     3661 caaacagatg ggctgggtcc agggcgcgat ctggagcaac gcccacgtgc gcgaggccgt
     3721 gacggcgatg aaggaaaagc gcggcgcgga atttccttcg ctgccgccgc tgcgctcgtt
     3781 ccgcgaactc tgaggatcag cgcctggcgg gatgtctggg tgtggtcttc gcgcctgctt
     3841 ttgccttgcc cttcggcttc ttcggcggca aggtcctggc gtaggccagg gcccgcgcta
     3901 tccagtcgcg aaactgcgcc ggcgggcaat cgagcgcatc ctcgatcgcc gcatactcct
     3961 tcatcacctt gcccatcacc tcgaacggcc gcgaggaagc ctgcgccggc agtcgcacga
     4021 tcagccgctg ctcgtgcagt cccgcgaaca tgttgccgtt gatgaacgcg caggggtagc
     4081 cgaacatctg cctgcgctcc acgccctgcg tcaccggcag gctcgccaga aagcgctcga
     4141 ccagggccgg gtcggatttt tgccaggtcg ccgtcagttg ctgctccttc ccattgcccc
     4201 tactgccgcg ccatcctgac gttgggcggc ggcgccatcg gatggctggt gcggaagggg
     4261 ttgatgtcaa gcccgccgcg ccgcgtgtag cgcgcataga ccacgagctt gaccggcttg
     4321 cagcgccgcc acaggtccat gaagatgcgc tcgacgcact gctcgtgcaa ttcgttgtgg
     4381 ttgcgaaagc tcaccaggta gcgcagcaat ccgccctggt cgatctgcgg gccgctgtag
     4441 cggatctgga tgctgcccca gtccggctgg ccggtgaccg ggcagttgct cttgagcagg
     4501 ttgctggtca gcacttcttg caccggctgg tcgccgaacg cggccgtgag caattccggc
     4561 gccggcgtgt actgggtgca ttcgatctcg agccggtcga gactcaggcc gtcgagctcc
     4621 cgcaccggct cgcgatcgaa ctgctccggc agcagcagcc gcaggcccac cgaggactgc
     4681 gtgggcgcac cgcgccagac ggcctcgccc acatcggcgc ggatgcggtc gcgcacctcc
     4741 tcggccgagg cgaaggcggt gccgctgaaa ctgttgaggt agagcttgaa ggacttgctc
     4801 tccacgatgt tgggtgtctc gcaaggcacc gtcacatggg cgatcgccac ctgcggcttg
     4861 ccgcggggcg taagccagct cagctcgaac gccgtccaca ggtcggcgcc gaaaaacggc
     4921 accgcgccgt gcaggcccag ctccgcccgc ttgggcaggc gcgaaatcgg aaacagcagc
     4981 gacgcgtcgt accggtccgc atagggtgcc ggtttgccga gctgcgattg gtcgggcgtg
     5041 ttcatcgtgc gagctgccct aggctggctt caggtgcggt accaacggtt cgagcaaccg
     5101 ctcgaccacg ccgggcggaa ggtcatgccc catgccttcg atacccagga agcgggcgcc
     5161 gggaatgcgg cgggccgtgt cgtggccgca ggcaaagggc accagggggt cttcccggcc
     5221 atgcagcacg agcgtgggcg tccggatggc cgccagctcg tccgcgcggc gggtatcggc
     5281 ggccacggcc gtcatctggc gcagggtgcc cgccggatga aagctgcgcc ggacggaggc
     5341 cagcaagcgc tcgcgcaggg ccgcctcgtc gagcgggaat ccggggctgc cgatcaccct
     5401 gaacagcttg acgaggtgct cgacgatggc ctgctcgcca ccgcctttgg ggcgactgag
     5461 caggacacgc aacacctgcg gcttcggacc gggcaggtag cgggcgccgc tcgaccccat
     5521 gatgctggcg aggctcagca cccggccggg cgccgccagg gcgacccgct gggcgatcat
     5581 cgcgcccatg ctgacgccca ccacatgggc ctgcgctatg cccagggcat ccagcacccc
     5641 cagggcgtcg gccgccatct catgcacgct gtagggcgcg cggacagcga acccggcccg
     5701 gaacttgagg ctctcccaca gcaggttggg cacgcccagc tggtcgaaat gctgggacag
     5761 gccgacgtcc cggttgtcga c
//
LOCUS       DASWIY010000292         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2055717, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000292 DASWIY010000000
VERSION     DASWIY010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2055717"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VGA59_10665"
     CDS             <1..259
                     /locus_tag="VGA59_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX9720183.1"
                     /translation="QADCFAGVWAHHAQNARQILEQGDVEEAMNAAAKIGDDALQGSR
                     SGAVVPESFTHGSSAQRQRWFSTGLKTGSVKACDTFGARQL"
     gene            372..1802
                     /locus_tag="VGA59_10670"
     CDS             372..1802
                     /locus_tag="VGA59_10670"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9720184.1"
                     /translation="MTASFSNLSLAEPLARAVAEMGYESMTPIQAQAIPVVLTGIDVM
                     GAAQTGTGKTAAFALPLLQRLLKNENTSTSPARHPVRALVLLPTRELADQVAQQIKLY
                     GKYTNLRSAVVFGGIDMKPQTAELKKGVEVLVATPGRLLDHIEAKNAVLNQVEYVVLD
                     EADRMLDIGFLPDLQRILSFLPKQRTTLLFSATFSAEIKRLANSYLQNPVTIEVDRPN
                     QEADTVEQHFYSVSDDEKRHALKQIVRQRGLTQAFVFVNSKLGCARLARSLEREGMNT
                     TALHGDKTQDERLKALDAFKQGKVDLLVATDVAARGLDIKDVPAVFNFDVPFNAEDYI
                     HRIGRTGRAGQSGLAVTFVSGSDTRLIGDIEKLLKRKIELEAIEFDEDRPRGRINDGR
                     RAWREVGEVGDPRDVLDAPPRERAPRQQREYRPPAAPRDPFFDQPYQPPVTEAPPAWE
                     AAVKPATRVSANIKPKRKVAALFKSE"
     gene            complement(1928..>2162)
                     /locus_tag="VGA59_10675"
     CDS             complement(1928..>2162)
                     /locus_tag="VGA59_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HEX9720185.1"
                     /translation="DAPPGYLPWFDVPGRKTADATIAFGHWSTLGFLQRPDVISLDTG
                     CVWGGALSALRLGAPGARHELIQVKCEQVRVPG"
BASE COUNT          397 a          742 c          705 g          318 t
ORIGIN      
        1 gcaggccgac tgcttcgccg gtgtgtgggc ccatcacgcg cagaacgcgc gtcagatcct
       61 ggagcagggc gacgtcgaag aggcgatgaa cgccgccgcg aaaatcggcg acgacgccct
      121 gcagggatcg cgcagtggcg ctgtggtccc ggagagcttc acccatggca gcagtgccca
      181 gcgccagcgc tggttttcga ccgggctgaa gaccggcagc gtcaaggcct gcgacacctt
      241 cggcgcccgc cagctgtaag ccgccgactc ggggcggggc cggccggcgc aaggcgaaaa
      301 cggcgtgctg cggcggtttc acgcgctgtc acagggatgt cagccgcgaa gtgcgacaat
      361 acccggttga catgacagct tccttttcca atctatcgct ggccgagccg ctggcacgcg
      421 ccgtagccga aatgggctac gagtccatga cccccatcca ggcgcaggcc attccggtcg
      481 tcttgaccgg catcgacgtg atgggcgccg cccagaccgg caccggcaag acggcggcct
      541 ttgcgctgcc gctgctgcag cgcctgctca aaaacgagaa cacgtcgacc tcgccggcgc
      601 gccatccggt gcgggctttg gtgctgctgc ccacgcgcga gctggccgac caggtggccc
      661 agcagatcaa gctctatggc aagtacacca atctgcgcag tgcggtggtg ttcggtggca
      721 tcgacatgaa gccgcagact gccgaactca agaagggcgt cgaggtgctg gtggccacgc
      781 cggggcggct gctggatcac atcgaagcca agaacgccgt gctgaaccag gtcgaatacg
      841 tggtccttga cgaggccgac cgcatgctgg atatcggctt cctgccggac ctgcagcgca
      901 tcctgtcgtt cctgcccaag caacgtacca ccctgctgtt ctcggccacc ttctcggccg
      961 agatcaagcg gctggccaac agctacctgc agaatccggt gaccatcgag gtggatcggc
     1021 ccaaccagga agccgatacg gtcgaacagc atttctacag cgtttccgac gacgagaagc
     1081 gccatgcgct caagcagatc gtgcgccagc gcggcctgac ccaggcgttc gtgttcgtca
     1141 acagcaagct cggttgcgcc cgcctggcgc gttcgctcga acgcgaaggc atgaacacca
     1201 cggccctgca cggcgacaag acccaggacg agcgtctgaa ggcactggac gctttcaagc
     1261 agggcaaggt cgacctgctg gtggccaccg acgtggcggc ccgcggcctg gacatcaagg
     1321 atgtgcccgc ggtgttcaac ttcgacgtgc ccttcaacgc cgaagattac atccaccgca
     1381 tcggccgcac cggccgggcc ggccagtcgg ggctggccgt gaccttcgtc tccggcagcg
     1441 acacgcgcct gatcggcgat atcgagaaac tgctcaagcg caagatcgag ctggaagcca
     1501 tcgaattcga cgaggacagg ccgcgcggcc gcatcaacga cggccgccgc gcctggcgcg
     1561 aggtgggcga agtgggcgat ccgcgcgacg tgctcgatgc gcccccgcgc gaacgcgcgc
     1621 cgcgacagca gcgcgagtac cgtccgccgg ccgcgcctcg tgacccgttc ttcgaccaac
     1681 cctaccagcc gcccgtcacc gaggcgccgc cggcctggga agcggccgtc aagcccgcca
     1741 cccgcgtctc ggccaacatc aagccaaagc gcaaggtcgc ggcattgttc aagagcgagt
     1801 gaagcttgcg gtggctgtca tcccgggcgt gacccgggac tgagggctgt catcccggac
     1861 tcgatccggg atccatgact tcagaattca tggattgcgg ggccgggcgc aatgacaagc
     1921 ggggctgtca tcccggcacc cgcacctgct cgcatttcac ctggatcagt tcatgccggg
     1981 cgcccggcgc gcccagccgc agcgcgctca acgcaccgcc ccatacacaa cccgtgtcca
     2041 gcgaaatgac gtcgggccgc tgcaggaagc ccagcgtcga ccagtgcccg aaggcgatcg
     2101 tcgcgtcggc ggtcttgcgg cccggcacgt cgaaccaggg caggtagccg ggcggcgcat
     2161 cg
//
LOCUS       DASWIY010000293         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1294869, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000293 DASWIY010000000
VERSION     DASWIY010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294869"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..923)
                     /locus_tag="VGA59_10680"
     CDS             complement(57..923)
                     /locus_tag="VGA59_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX9720186.1"
                     /translation="MSSPTPDVAARIARLFVYPVKSCAGVELTQALLTESGLDLDRAW
                     MVVDDKGEFVTQRELPRMALVRPQLKHSEVVLRAPGMLALHLAIDAVEQPAKVRLWDE
                     PVEAFDMGDVAAQWFSDFLQRKLRLVRFDPDHRRLSSPQWTGGLEVPNQFSDGFPLLV
                     LGEASLAELNDKLVAAGHGAVGIERFRPNIVLAGLQAQDEDRLDVLRVATGGEEVLLR
                     AVKPCPRCPIPDVDPATAQIDPQVSDTLRTFRKNERLQGAVTFGMNAIVLQGAEQTLR
                     VGQSVTAGWRFD"
     gene            1003..2121
                     /locus_tag="VGA59_10685"
     CDS             1003..2121
                     /locus_tag="VGA59_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9720187.1"
                     /translation="MAPALDVCIRGDGVVGRTLALLLARDRLRVGLVRQSVAAGGATS
                     DVRAYALNSASKDLLEMLRAWPDAAHATPVVAMQVHGDEGGQVQFSAQAQGVPALAWI
                     VDVQALQERLGDALRFQPLVQSLDAPQPAALTVVCEGKASSTRAEFGVQYDVTQYPQR
                     AIAARLNCEKPHGQIARQWFADGEILAFLPLGGSGPLSQGNSVAVVWSVQEQRAPDLL
                     AMEGHAFEAQLEAVSHHGLGKLTLASERAAWPLQLARADRWCGPGWALAGDAAHTVHP
                     LAGQGLNLGLADARELAQVLHQREGWRSVGELKLLRRYERSRKADVLAMGVATDSLQQ
                     LFAQQAEPWGMLRNWGMTGFDRSGPLKQWVARQAMGRV"
     gene            2144..>2563
                     /locus_tag="VGA59_10690"
     CDS             2144..>2563
                     /locus_tag="VGA59_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEX9720188.1"
                     /translation="MHLIKKAALAALLASGLVAGVAHAQEAAIRKNLPERLPQMGKID
                     EVTKTPMPGLFEVRVGTDVYYSDAEGNFLFHGQLIDTRQKRNLTEERQEKLLAIDFAA
                     LPVKDAFTIVRGNGSRKIAVFEDPNCGYCKRFERDLQK"
BASE COUNT          411 a          848 c          909 g          395 t
ORIGIN      
        1 gcgcgagtcc gacagcctgc cgccgtcctt gcaaccgccc gcggccgctg acgcgctcag
       61 tcgaagcgcc agccggccgt gacgctctgg cccacgcgca aggtctgctc cgcgccctgc
      121 agcacgatcg cgttcatgcc gaaagtcacc gcaccctgaa ggcgttcgtt cttgcggaag
      181 gtccgcagcg tgtcgctcac ctgcggatcg atctgcgcgg tggcgggatc gacgtcggga
      241 atcgggcaac gcggacaggg cttcacggcc cgcaacagca cttcctcgcc gccggtggca
      301 acccgcagca cgtccagccg gtcttcgtcc tgcgcctgca ggccggcgag gacgatgttg
      361 gggcgaaaac gctcgatgcc cacggcgcca tggccggccg ccaccagctt gtcgttcagc
      421 tcggccagtg aagcttcgcc caggaccagc agcggaaaac cgtcgctgaa ctggttgggc
      481 acttcgagcc cgccggtcca ctgcgggctg gacaggcggc ggtggtcggg gtcgaagcgc
      541 accagccgaa gtttgcgctg caggaagtcg ctgaaccatt gcgccgccac gtcgcccatg
      601 tcgaaggctt cgaccggctc atcccacagc cgcaccttcg ccggctgctc caccgcatcg
      661 atggccaggt gcagggccag catgcccggt gccctcagca ccacctcaga gtgcttgagc
      721 tgaggtcgca ccagcgccat gcgcggcagc tcgcgctggg tcacgaattc gcctttgtcg
      781 tccaccacca tccaggcgcg gtccaggtcc aggcccgatt cggtcaggag agcttgcgtc
      841 agttccacgc cggcgcagga cttgacggga tacacaaaca ggcgggcgat gcgggcggcg
      901 acgtcgggtg tgggagaact catgggcttg gggcgtggat gctaggggct tggcggaaga
      961 gaaccagccg cgattgtgcc cggctcctac aatcccccca tgatggcccc tgcccttgat
     1021 gtttgtattc gcggcgacgg cgtcgtcggg cgcaccctgg ccttgctgct ggcgcgcgac
     1081 cggctgcgcg tcgggctggt gcggcagagc gttgcggcgg gcggcgccac cagcgacgtg
     1141 cgtgcctacg ccctgaactc ggcctccaag gacctgcttg aaatgctgcg tgcctggccc
     1201 gatgccgcgc acgccacccc ggtcgtggcc atgcaggtcc atggcgacga gggcggacag
     1261 gtccagttca gcgcgcaggc ccagggcgtg ccggcgctgg cctggatcgt cgacgtccag
     1321 gcgctgcagg agcggctggg cgatgccttg cgctttcagc cgctggtgca atcgctggac
     1381 gcgccccagc cggcggcgct cacggtggtg tgcgaaggca aggccagcag cacccgggcc
     1441 gagttcggcg tgcagtacga cgtcacgcag tatccgcagc gcgcgatcgc ggcgcggctt
     1501 aactgtgaaa aaccccatgg gcagatcgcg cggcaatggt tcgccgacgg cgaaatcctc
     1561 gctttcctgc ccctgggcgg ctccggcccg ctgtcgcagg ggaactcggt ggccgtcgtc
     1621 tggtcagtcc aggagcaacg cgcgccggat ttgctggcga tggagggtca cgccttcgag
     1681 gcgcagctgg aggcggtgag ccaccatggc ctgggcaagc tgaccctggc gagcgagcgg
     1741 gcggcctggc cgttgcagct ggcgcgggcc gaccgctggt gcggaccggg ctgggcgctg
     1801 gcgggcgatg cggcgcacac ggtgcatccg ctggccggcc agggcttgaa cctggggctg
     1861 gccgatgcga gggaattggc ccaggtgctg caccagcgcg agggctggcg cagcgtgggc
     1921 gagctgaaac tgctgcgcag gtacgagcgc tcgcgcaagg ccgacgtgtt ggcgatgggt
     1981 gtcgccacgg acagcctgca gcagcttttt gcccagcagg ccgagccttg ggggatgctg
     2041 cgcaactggg gcatgacggg gttcgatcgc agcggtccgc tcaagcaatg ggtggcgcgg
     2101 caggcgatgg ggcgggtttg attctggaag taatggaaca acaatgcatt tgatcaagaa
     2161 agccgcgctg gcggccctgc tcgcatccgg gctggttgcc ggggtcgcgc acgcccagga
     2221 agcggccatc cgcaagaacc tgcccgagcg gctgccgcag atggggaaga tcgacgaggt
     2281 caccaagacc cccatgcccg gcctgttcga ggtgcgcgtc ggcacggacg tgtactacag
     2341 cgacgccgaa ggcaatttcc tcttccacgg ccagctgatc gacacccggc agaagcgcaa
     2401 cctgaccgag gagcgccagg agaagctgct ggcgatcgac ttcgcagccc tgccggtcaa
     2461 ggacgctttc accatcgtcc gcggcaatgg ctcgcgcaag atcgccgtgt tcgaggaccc
     2521 gaactgcggc tactgcaagc gcttcgagcg cgacctgcag aaa
//
LOCUS       DASWIY010000294        21602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3166012, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000294 DASWIY010000000
VERSION     DASWIY010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 21602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21602
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3166012"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(440..1489)
                     /locus_tag="VGA59_10695"
     CDS             complement(440..1489)
                     /locus_tag="VGA59_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9720189.1"
                     /translation="MSEITRVGVDLAKQVIQVHAVDQAEAVVTRRAVPRRAFLDWCAR
                     LPPGCLVAMEACTGAHHWARLLSKLGLKVRLIAPHLVTPYRRQGKTGKNDANDAAAVC
                     EAASRPDMHFVPVKTVEQQGILSVHVMRQGWEKDRTACINRIRALLAEFGLVLAKGPE
                     KLRLQLPELLEDATNDMPGVTRLAIQRAVQHWRQLDEDIGWCDQQIQRHARTEPRAQQ
                     AQTLLGIGPIGASALVASVSDFSQFTSGRQFAAWLGITPSQNSSGGRTRLGGITKHGD
                     EYLRMLLVTGAKAAIHRGKPREERVWLWAKQLSERAGWQKAAVALAAKNARILWAMFT
                     RGQSFVPNHTSVKPT"
     gene            complement(1726..2919)
                     /locus_tag="VGA59_10700"
     CDS             complement(1726..2919)
                     /locus_tag="VGA59_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX9720190.1"
                     /translation="MAQFIQVDEPPTPQAGKGFALWALGFRPFYLLASAFAALSIGLW
                     ALQFSGWLGRPYLQGPLWHAHEMLFGFTLAVVVGFLFTAGSNWSNRPTPTGIPLAAMA
                     ALWVAGRVLVLTPFGWAAAIVNAAFPLAAAIALAIPFIASGNRRNYFFVGLLVLMSIA
                     VLAVHLEQLGVLQVPGWVGIQVALDAVLFIVAVMGGRVIPMFTNNGVWGANATRRPVV
                     EKLALGSVLVLLVADALQVQGAPLAVLAVACAAAHLARWFLWQPWKTLRTPIVWVLHA
                     AYLWIPVHLLLRAMAVGGLVTPSAASHALTVGAIGGMIIGMMTRTARGHTGRPLQADR
                     YEVACYAMVLLAALVRVLVPLAAPAQTVNAVVWSSALWSAAFALYTVRYWPILTRPRL
                     DGRPG"
     gene            complement(2919..4019)
                     /locus_tag="VGA59_10705"
     CDS             complement(2919..4019)
                     /locus_tag="VGA59_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720191.1"
                     /translation="MAVAARPLFLVLVAVSLLTGIAGGLLRVGVMLPSTANTAWLGQA
                     GLQHAALMICGFLGTVIGLERAVAVKLPAAFVAPLASSLGALCLLLGQTTHGAWLGVA
                     AAAVFTAVNVVVVRRQWAGHTLLLLAAAAAWLAGSLLFALGQGAEATLPWWFAFLVMT
                     IAAERLEMTRLMRRRPAAPVLLVAVLAVMVLGCVASAVSVQVGGVLYGVSLVALALWL
                     GTFDIARRTVFAQGLSRYMAVCLLTGYIWLAIAGAAWAATALGFPLRDVALHALGLGF
                     IFSMIMGHGPVILPAIARVKLQFGGFFYVPLLALHLSLALRLGWGAIEPEQRAIGAIS
                     NGLSIALFAATVVGAAIAWKIRYGADGARKTS"
     gene            complement(4055..4426)
                     /locus_tag="VGA59_10710"
     CDS             complement(4055..4426)
                     /locus_tag="VGA59_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX9720192.1"
                     /translation="MKTLEDFASDDALEPKPQYPYEGPEALRQPILDALTRVVDPEVA
                     MSIVDVGLIYGVTVNDEKVHVLLTMTSAACPVTDVIKEEVETELDRLAPPELLVHVEL
                     VWDPPWTMDRMSERARRFMGW"
     gene            complement(4423..4893)
                     /locus_tag="VGA59_10715"
     CDS             complement(4423..4893)
                     /locus_tag="VGA59_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9720193.1"
                     /translation="MKLTAFTDYSLRVLIYVAAQPERRATIADIAEAFEVSENHLTKV
                     VHFLGRNGWLATVRGKGGGLELPMPPELIEVGKVVRETEGAGLPADCFGKNGDHCCIA
                     GICRLRGVLGEAVGAFYAVLDKYTLADLVHNRQSLANVLFTEQVMAPPAAGRAA"
     gene            complement(4922..5173)
                     /locus_tag="VGA59_10720"
     CDS             complement(4922..5173)
                     /locus_tag="VGA59_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX9720194.1"
                     /translation="MTTIPIFSNVHPFDARGVAKRFRHAAIFGALQSLNAGETMRFVN
                     DHDPLPLLAQLQQAFGDTIGIEYVRREPGDIVIDFQMRV"
     gene            complement(5430..5717)
                     /locus_tag="VGA59_10725"
     CDS             complement(5430..5717)
                     /locus_tag="VGA59_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX9720195.1"
                     /translation="MNTATATRIDVRQLACEQRHSLIFSTFGSLAAGKALELVNDHDP
                     KPLRNVFQAQYPGKFGWEYLEQGPDTWRVAITKLADAHGSSGSCCGSCGGE"
     gene            5863..6270
                     /locus_tag="VGA59_10730"
     CDS             5863..6270
                     /locus_tag="VGA59_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase subunit alpha"
                     /protein_id="HEX9720196.1"
                     /translation="MTSPSHFELLLQAAASQPEPQRLLFVFAEAELPADASPAQRARF
                     EAGQGGALTPLACVDKAVDDLVSFEALVAESRHASPPWQVVFIAGLSGQGGRPPSSVQ
                     VDSALQAMVENVRAGRFGGLLAFGPDGEPLSFS"
     gene            complement(6296..7213)
                     /locus_tag="VGA59_10735"
     CDS             complement(6296..7213)
                     /locus_tag="VGA59_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX9720197.1"
                     /translation="MALACAALVLAITSLSAFIRLSQAGLGCQPWPQCYAQSLEAAGQ
                     RAAPPTEGAVAVARITHRVVAVAALLLVIALVMMTLSKVPVLWREGRMALGLLALALF
                     LAILGRWTTDARVPAVMLGNLLAGFAMFALSWRLAESTHHHPGAIAAPHSLTRWARVG
                     LALLVAQIILGGLVSAGHAGLSCPQLLACDVAGGSWQLLNPWHETPLDVADPVNPAGA
                     LVHGLHRAGAVLVAAVLLPLGVAAWRSGRRAGAAVILLLVVQAALGVLLVTGGLPLPM
                     ALAHNVTAALLLAAVLGLAADGGRLPERP"
     gene            7422..7577
                     /locus_tag="VGA59_10740"
     CDS             7422..7577
                     /locus_tag="VGA59_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720198.1"
                     /translation="MSEEVEDTGPVPLMQQLLDNPFLLLFLGVLIPMVVYTLWGVIDI
                     LTIPLAK"
     gene            7588..8127
                     /locus_tag="VGA59_10745"
     CDS             7588..8127
                     /locus_tag="VGA59_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HEX9720199.1"
                     /translation="MSAILPPAERLWWKHPLDRVEGTWIAIAFAWCMVMFFMMIYWHA
                     YGKQNLSTETYKTTPDQFSAKAQAVVDKYTVRTETAEKIPVVAPPEGSDVYLVARLWS
                     WWPILELEKNKTYKLHLTAMDYNHGFSLQPANINIQVVPGYEHVIKVTPNQSGTFSIV
                     CNEYCGINHHSMVGRIYVK"
     gene            8138..9868
                     /locus_tag="VGA59_10750"
     CDS             8138..9868
                     /locus_tag="VGA59_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9720200.1"
                     /translation="MSATLPIRTTPPHATSYRTCPRSGLQFELQAERLMIANAVIAVV
                     MLLVGGVLAIGVVLTRWPAVHWLAADTFYMVLTAHGIDMLIFWIIFFEIAVLYFCSST
                     LLRCRIATPNLAWLALALMVIGAVTNNVAVFQGGSSVMMTSYVPMMASPAFYLGLILF
                     AVGALIGCFVFFGTLVIAKRDKTYQGSVPLVTFGAITAAIIAVFTIASGAIILIPTFL
                     MSIGVIQSVDPLVYRTIWWAFGHSSQQINVAAHISIWYAVAAIAFGAKPMSERVSRGS
                     FLLYILFLQLASAHHLLADPGLSTGWKIVNTSYFMYFAVLASMIHGLTIPGAIEVAQR
                     AKGYNKGLFEWLRKAPWGNPVFSGMFISLIGFGFLGGISGVMMGTEQLNMLIHNTIYV
                     PGHFHATVVIGTTLTFMALTYFLIPVLFRREMINPGLAKLQPYIFGLSMYFFVLVMMG
                     AGTLGVSRRHWDMALTGAALGYEWPGAAYLMMALVGIAGMAAIIGGGIYIYITVGSLL
                     WGKKLDTGAVSPMFTPIPPTAKTAVAQSYGSAGFAAPGTFMLAMLFLVAFVLYYFINW
                     KYLGQLWGLS"
     gene            9882..10781
                     /gene="cyoE"
                     /locus_tag="VGA59_10755"
     CDS             9882..10781
                     /gene="cyoE"
                     /locus_tag="VGA59_10755"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX9720201.1"
                     /translation="MNTTTLGGTRRRTAQVARDVLSLFKLRIGVLIMVTALVGLAVTP
                     GAALGLGQVLVLALSVLVASAAAGAFNQYVEHDSDALMARTRRRAFVTGALSHHPAWL
                     LLIGVLLAGSVAAAWFTLNAMAAMYVFLGAFFYAVVYTVWLKRRSALNIVVGGLAGSF
                     AVLAGAAAADPTPGPLPLLLALVLFLWTPPHFWSLAIANRDDYAAAGVPMLPVVVGIE
                     RAARIVFASTAALVVASLLPLAFGAGPIYAVGAIGGGFHFLRKSWLLARRPDRASAMG
                     SFFASLVQLSALLVAATLDGLLR"
     gene            10949..11803
                     /locus_tag="VGA59_10760"
     CDS             10949..11803
                     /locus_tag="VGA59_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX9720202.1"
                     /translation="MRFPRLAGAAQLAATLSLAGALLQPLAAPAQEAARVPGLDQQAA
                     IKASQAVLGQSIGDYTLLDREGRPVRLASYRGKPLLVSFIYTGCFQVCPTATRSLDEA
                     VRALQGRFGDNQFNVVSIGFNQPADSPQALKAFAAQHRISRPNWDFLSPPSPIVGPLT
                     RDFGFLYQATPAGFDHVLQVSLVDAQGRIARQVYGDRVAADALGEPLKQMLIGAPLPQ
                     GIRLDDIIDQVRLLCTVYDPKTGTYRVDYSLAIEFAGGLTFIIAVALYMLNEWRSRRR
                     ARRRAQGA"
     gene            11847..13436
                     /locus_tag="VGA59_10765"
     CDS             11847..13436
                     /locus_tag="VGA59_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HEX9720203.1"
                     /translation="MAKLAPAGPSVLHLAQPTPPERPGAKAAYRRLENAFDAAFGGAL
                     NPLKHLGALAFLLFWLLAVTGIVLYVVLDTSAHGAYRSIDELSRDPWSFGSALRGLHR
                     YAADAFVFAALAHLAREWLLGRYSGFRAFSWLTGVPLLPLAYVCAIGGFWLNWDRLGQ
                     FSAIATAEWLDALPFLASPLARNFLGGAVNDRLFSLFVFVHLGVPLLLVFGLWFHIQR
                     ISRAEVFPPRALALGTTAALLALALALPVRSQGPADLAVVPGRLSLDWFLLFIHPLTG
                     ATSAAFVWALLTAAIVGLFALPFLRRRAAPPVAQVDPANCNGCRRCADDCPYGAVTMV
                     PHPNGRPGAQLAQVKAELCASCGICVGACPSATPFRKGETLVTGIDMPQWPIDDLRRR
                     LVRGLAAMRAERRLVVFGCDQGARVQALAAPDVLPFSLVCAGMLPPSFVEYALRAGAD
                     GVLVAGCREGGCEFRLGQRWTAQRLLGLREPRLRAAMTAGRWATLWADAGDEAALQAA
                     LDKLRREAGMAAPATLAEAPA"
     gene            13433..13936
                     /locus_tag="VGA59_10770"
     CDS             13433..13936
                     /locus_tag="VGA59_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720204.1"
                     /translation="MKRVMSWAGQVLLYGLFAVFVGVFSSWPAYEALPPDQALVKVSF
                     IHHGQRVAECRPYTAEELARTAPNMRTPMKCERERSPVTIEVDLDGATVYRRVAAPSG
                     LSRDGASSVYHRIAVPAGEHRIAVRLKDSASARGFTHTRDATLTLKPAQVLVIDFDAE
                     KGGITLS"
     gene            13933..15378
                     /locus_tag="VGA59_10775"
     CDS             13933..15378
                     /locus_tag="VGA59_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9720205.1"
                     /translation="MSAVPTSLAGPLAQRPLPAVQSATDYSFTVPADARLALARGWLW
                     LGMAALIGSGLFSVLLVLSRTPGVNELLPVADFFRVALVVHVDLSVLVWFMALAGMLW
                     TLNGSARRMGWGWCGLALAAAGAAAMSIAPFSGPGEAIMANYIPVLTAPLFMIGLLAF
                     AAGVAVLVLRSLANAPRLGLEFDGRGALRFGLNAAAVATAVALLSFGWSYAVLPGDLS
                     GKAYYEILFWGGGHALQFTWTLLMLVAWLWLAGACGARVPLSPRVTLLMFALALVSVF
                     VTPYAYLAYDIASVEHRALLTWAMRLGGGVAILPIGLAVVFGLWNAPPLDAAARPLRA
                     ALLSSVLLFAAGGVIGIFISGSNVRIPAHYHGCIVGVTLALMGLVFKLLPALGYGAPR
                     GRLATSQPWLYAVGQLMHIVGLVWSGGYGVQRKVAGAEQVLRSPAEIAGMGLMGLGGA
                     IAIVGGLLFVVVVLRAMRHARVPAGSLQAAR"
     gene            15378..17306
                     /locus_tag="VGA59_10780"
     CDS             15378..17306
                     /locus_tag="VGA59_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX9720206.1"
                     /translation="MKRLQALLQWLFMRLEALFNAAFGDRLNPLYHLGAITFFLFWVV
                     GASGLYLYVFFETGVAEAYSSVEALTHRQWFAGGILRSVHRYASDAMVVTMGVHMLRH
                     FAFDRFHGFRWFSWITGVSLIWLVYISGINGYMLPWDRLAQFVIVTSFEWLDWLPTFG
                     GTLMRNFVHANSVGDRFFSLLSFMHIGLPLIVLLLMWVHVQRVPKARTTPPRPIVASL
                     LATLLVLSLVRPALSQGGAADLGTAVSSLQLDWFYLALFPLLVAWPLGQVWALMAGGT
                     ALLVALPLWPELFRRNRHREYQLVVHSADGAATQLRLRDGETLLEAGLREGLALPYEC
                     RNGGCGVCLCSVEHGAVSHRPYQRSALPDALKAQGKALMCCAVPMGDMVIEVEGLAHG
                     GAATAKLHTGRVVALERLAQDVMRVLIALPEGQRVAFTAGQYINVLLDDGERRAFSFA
                     NAPHENELVELHVRRIPGGRFTGHVFEAMRVGDTLRFEGPLGSFTLNESARPILFVAG
                     ATGFAPVKSIIEDAFHRGLHRPMRLYWGVRRREDLYLLDLAERWQREHENFLVVPVLS
                     HPEAGDEWHGRTGLVHEAMLADFGDLVGYEVYVCGSLRMVEVAVPAFIAQGLNEDACF
                     SDAFLPSAEVSQPAGLVA"
     gene            complement(17314..18798)
                     /gene="ubiD"
                     /locus_tag="VGA59_10785"
     CDS             complement(17314..18798)
                     /gene="ubiD"
                     /locus_tag="VGA59_10785"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEX9720207.1"
                     /translation="MAYRDLRDFMAQLEGLGELRRVSQPVSPHLEMTALCDRVLRAGG
                     PAVLFERPTGHTIPVLGNLFGTPARVARAMGVNDLRELRPFGAALAALKEPEAPKGFK
                     EMMGLSGLLKTLWHMAPRVVSGAPCQEVVWEGADVDMARLPVQHCWPGDVAPLVTWGL
                     VVTRGPNKGRQNLGIYRQQVISRNKLIMRWLAHRGGAQDFREHGIAHPGEPYPVAVAL
                     GADPATLLGAVTPVPDSLSEYQFAGLLRGGRSEIAKAVGIDLQVPATAEIVLEGHIQP
                     DASHPSGYEHALEGPFGDHTGYYNEQAWFPVFTIDRITMRRDPIYHSTYTGKPPDEPA
                     VLAMALNELFIPLLQRQFPEITDFYLPPEGCSYRMAVVQIRKAYPGHARRVMMGVWSH
                     LRQFMYTKFIVVVDEDVDVRDWREVIWAMTTRMDPARDTMLVENTPIDYLDFASPVSG
                     LGSKMGLDATNKWPGETPREWGRPIAMDKAVQARMDEILQGLGL"
     gene            complement(18809..19414)
                     /locus_tag="VGA59_10790"
     CDS             complement(18809..19414)
                     /locus_tag="VGA59_10790"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX9720208.1"
                     /translation="MKTAPPALIRMIVAISGASGAVYGVRLLQVLRGIDGVETHLVVS
                     DAGWRNLQQEQGLDRGSAEALAHRVHDVRDVGAAIASGSFRSSGMVIAPCSMRTLGAV
                     AHGLGDNLITRCADVMLKERRRLVLMVRESPLNLAHLRNMVSATEMGAIVCPPMPAFY
                     LRPQSVAEIVDAGVARVLDLLELPHNLAPRWEGLSPAGASL"
     gene            complement(19418..19927)
                     /locus_tag="VGA59_10795"
     CDS             complement(19418..19927)
                     /locus_tag="VGA59_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX9720209.1"
                     /translation="MNYTPSHAVPRPLGMLLARLPAYPGSVLLSTALNLGLADQLQDD
                     VRQLLAGKKLRIHVRDARLTFDFTWTGQGFAACAGGNEADLTIGASAYDFLQLAQRRE
                     DPDTLFFSRRLSMEGDTELGLVVKNTLDALELPVFELQRWAPHRVLARLAPRTHSSDI
                     GPRHHGSGK"
     gene            complement(20072..20689)
                     /locus_tag="VGA59_10800"
     CDS             complement(20072..20689)
                     /locus_tag="VGA59_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX9720210.1"
                     /translation="MYAGERFNSISHLVGAGLAVGGTALLVVLAAQLGDPWKIVSFSV
                     YGAMLVALYVFSTLYHSVRGRAKNVLRKIDHCSIYLLIAGSYTPFTLVSLRGPWGWSL
                     FGAVWTLALLGIAQEIWLAKGSRVLSLVIYIVMGWLALVAVLPLWQALKPAGFAWLLA
                     GGAMYTIGILFYATDHKLRHGHGIWHLFVLGGSACHFFAVLLHVA"
     gene            complement(20689..21582)
                     /locus_tag="VGA59_10805"
     CDS             complement(20689..21582)
                     /locus_tag="VGA59_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX9720211.1"
                     /translation="MKLSMGAVQFFWPRDKVFEFYRQLADQPVDVVYLGETVCSRRHE
                     LRLADWLEIAGRLRQAGKDVVLSTQVLLESGSDVSSMHKIAANGEYLVEANDMGAVHR
                     LAGRGAFVAGPHLNLYNLPTLEWMAKLGASRWVIPLEMKRDDLALLQPARPAGLQTEV
                     FAYGRMPLAFSARCFTARHRNLPKDDCRFSCMEHPDGLLLKTRESEEFLVLNGTQTQS
                     ARIYNLIDEIEDMRALGVDMLRISPQANDTPDVIALFDQARKKALAPREALARLAPLM
                     PGEACNGYWHGRPGLEQGQAA"
BASE COUNT         3371 a         7414 c         7126 g         3691 t
ORIGIN      
        1 gacacgtacc aggagttcgt ggagcgccat cgcagggcga tggagcagga caagtgacgc
       61 ccgcagcgcg tgcgtcatcc cgggcttgac ccgggattca tagcggctca cgcagagcgc
      121 cgctccagtg acatggatgc ctactggggc ttccctcctt gtcaagcgat ttcgttgata
      181 cggggttttc tccttgctaa gcaatatggg cgacggctca ggcgcggcga gcgaactggg
      241 gcagactgca aacctactgt cggccttgtt gacaggcacg agggcctgtc gggcccagat
      301 acgaaccgct cagcggactc tcctattcgg gtctcgtcgc acgggtgcga cacggcatcc
      361 atcgagtttg tccgctgccg gtctgacctg ttcaatcgat ctacgctatt gccgtcttgc
      421 tgataattgg tgatttggtt caggtcggtt tgacgctggt gtgattgggc acgaacgact
      481 ggccgcgggt aaacatggcc cacaggatgc gggcgttctt ggctgccaaa gcgacggcag
      541 ctttttgcca gcctgctcgc tcactgagct gcttggccca tagccacact cgctcctcgc
      601 gcggcttgcc gcggtggatg gcggctttgg cgccggtgac cagcaacatc ctcaggtact
      661 cgtctccgtg cttggtgatg ccgcccagac gagtcctgcc gccgctggag ttttgcgagg
      721 gtgtgatccc aagccaggcg gcaaactggc gcccgctggt gaactggctg aagtcgctga
      781 cgctggcgac caacgccgat gcgcctattg ggccgatccc gagcagcgtt tgggcctgct
      841 gggctcgcgg ttcggtgcgg gcgtggcgct ggatttgctg gtcgcaccag ccaatgtcct
      901 catcgagctg gcgccagtgc tgcaccgctc tttggatggc caatcgggtg accccgggca
      961 tgtcattggt ggcgtcctcc agcagctcgg gcagctgcag ccgcagcttc tcggggcctt
     1021 tggccagcac caaaccaaat tcggccagca gagcgcggat gcggttgatg caggccgtgc
     1081 ggtccttctc ccagccctgg cgcatcacgt gtacgctcag gatgccttgc tgctcgaccg
     1141 tcttgacggg cacgaaatgc atgtccgggc gagaagcggc ctcgcaaacg gccgcggcgt
     1201 cattggcgtc gttcttgcca gtcttgcctt gccggcgata gggagtcacc aagtggggtg
     1261 cgatcagacg caccttcagg cccagcttgc tgagcaggcg cgcccaatgg tgcgcgccgg
     1321 tgcacgcctc catcgccacc aagcagcctg gcggcaaccg cgcgcaccag tccaggaagg
     1381 ctctgcgggg aactgcgcgg cgggtgacca cggcctcggc ctgatcgact gcatgcactt
     1441 gaatgacctg ctttgccaag tcaacgccga ctcgtgtaat ctcgctcatg ggacttctcc
     1501 tttccgttca gattgacgtt tttcccaaca tcaatcttgg cgctttgacg ccgttcaagg
     1561 aaggtgggaa gtcccctcgt attcgggtca aacccggcat gacaactccc atcaaatctc
     1621 cggacagcag tgggcttgac cggcaatcca cttctcttca accccggact tgatccgggg
     1681 ccggatgcca atcgtacttc aactgcgatc agggccggtt cggccctacc ccggccgccc
     1741 gtccaggcgc ggccgggtca ggatcggcca gtagcgcacg gtgtaaagcg cgaacgccgc
     1801 cgaccacagc gcggacgacc agaccacggc attgacggtc tgcgccggcg cggcaagggg
     1861 tacgagaacg cgcaccagcg ccgccagcag caccatcgcg tagcaggcca cctcgtaacg
     1921 atccgcctgc agcggccggc cggtgtggcc gcgcgccgtg cgggtcatca tgccgatgat
     1981 catcccgccg attgcgccca cggtcagcgc gtgcgacgcc gcagaaggcg tcaccaggcc
     2041 tcccacggcc attgcgcgca gcagcaagtg caccggaatc cagaggtagg cggcatgcag
     2101 cacccagacg atcggcgtgc gcagcgtctt ccagggctgc cacaggaacc agcgcgccaa
     2161 gtgcgcagcg gcgcaggcga ccgccaggac cgccagcggc gcgccctgca cctgcagcgc
     2221 gtcggccacc agcagaacca gcaccgaacc cagggcaagc ttctcgacga ccgggcggcg
     2281 cgttgcgttc gctccccaca cgccgttgtt ggtgaacatc ggaatcacgc gcccgcccat
     2341 caccgcgacg atgaacagca ccgcgtccag cgccacctgg atgccgaccc agccgggaac
     2401 ctgcagcacc ccgagttgtt ccaggtgcac cgccagcacc gcaatggaca tcagcaccag
     2461 caggcccacg aagaagtagt tgcggcggtt gccggaggcg atgaagggaa tggcgagggc
     2521 gatcgcggca gccagcggaa acgcggcgtt gacgatcgct gccgcccagc cgaaaggcgt
     2581 gagaacgagc acgcggccgg ccacccagag cgcggccatc gcggccagtg gaatacccgt
     2641 gggcgtggga cggttggacc aattgctccc ggccgtgaag agaaagccga ccaccaccgc
     2701 gagggtgaag ccgaacagca tctcgtgcgc atgccacagc ggcccctgca ggtagggccg
     2761 gcccagccag ccggaaaact gcagcgccca caggccgatc gacagcgcgg caaacgcgct
     2821 ggccagcagg tagaacgggc gaaagccgag cgcccagagc gcgaagccct tacccgcctg
     2881 cggagtcggc ggctcgtcga cttggatgaa ctgtgccatc agcttgtctt tctggcgcca
     2941 tcggcgccgt atcggatttt ccatgcgatg gccgccccga ccacggtggc ggcgaataat
     3001 gcaatggaga gcccattgga aattgctccg atcgcacgct gctccggctc gatcgcaccc
     3061 cagcccagcc gcaaagccag cgacagatgc agcgccagca acggcacata gaagaacccg
     3121 ccgaactgca gcttgacgcg cgcgatcgcc ggcaggatca ccggcccgtg gcccatgatc
     3181 atgctgaaga tgaatcccag ccccaaggca tgcagcgcca cgtcccgcag gggaaagccg
     3241 agtgccgtcg ccgcccacgc ggcaccggca atggccagcc agatataccc ggtcagcagg
     3301 cacaccgcca tgtaacggct caagccctgg gcaaacacgg ttcggcgcgc aatgtcgaag
     3361 gtgcccagcc acagcgccag cgcaaccagg gacacgccgt acagcacgcc gccgacttgc
     3421 accgaaacag cggaggccac acagcccagc accatgaccg cgaggacggc cacaagcaag
     3481 accggcgcgg ccggccggcg ccgcatcagc cgtgtcattt cgaggcgctc ggccgcgatc
     3541 gtcatgacca ggaacgcaaa ccaccagggc agcgtcgcct ccgcgccctg tcccaatgcg
     3601 aacaacaggc tgcccgcgag ccaggcggcc gcggcagcga gcagcagcag cgtgtggccc
     3661 gcccattgac gccgcacgac cacgacgttg acggcggtga atacggctgc ggccgccacg
     3721 cccagccagg ccccgtgagt ggtctgcccg agcagcaggc acagcgcgcc gaggctggac
     3781 gccagcggcg ccacgaaagc ggcgggcagc ttgacggcga ccgcgcgctc cagcccgatc
     3841 acggtgccca gaaaaccgca gatcatcaga gccgcgtgtt gcaggcccgc ctgaccgagc
     3901 caggcggtgt tcgctgtcga tggaagcatc acgcccaccc gcagcaggcc gcccgcgatg
     3961 ccggtcaaga gcgaaacggc gaccagcacc aggaacagcg gccgcgcggc gaccgccatg
     4021 gcggatggct ttcgccggga cgacgtgcgc ggcatcacca gcccatgaaa cggcgcgcgc
     4081 gctcgctcat ccggtccatg gtccatggcg gatcccaaac cagctcgacg tgcaccagca
     4141 gctccggcgg agcgaggcgg tccagctcgg tttcgacctc ttccttgatc acgtcggtga
     4201 cggggcaggc agccgacgtc atcgtcagca gcacatgcac cttctcgtcg ttcacggtca
     4261 cgccgtagat cagcccgaca tcgacgatgc tcatcgccac ttccgggtcc accacccgcg
     4321 tcagcgcatc caggatgggt tggcgcaacg cttcggggcc ctcgtacggg tactgaggct
     4381 ttggctccaa cgcgtcgtcc gatgcgaaat cttccaatgt cttcatgctg ccctcccggc
     4441 ggccggtggt gccatgacct gctccgtgaa aagcacattg gcgagcgact ggcggttgtg
     4501 caccaggtcg gccagcgtgt atttgtccag cacggcgtag aaagccccga cggcctcgcc
     4561 cagcacgccg cgcagacggc agatcccggc gatgcagcaa tggtcgccat tcttgccgaa
     4621 gcaatccgcc ggcagcccgg ccccttcggt ctcgcgcacc accttgccga cctcgatgag
     4681 ctcgggcggc atgggcagct cgagcccgcc ccctttgccc cgcaccgtcg ccagccagcc
     4741 gttcctgccg aggaagtgca ccactttggt caggtggttc tccgaaacct cgaacgcctc
     4801 ggcgatgtcg gcgatggtgg cgcgccgctc gggctgcgcg gccacgtaga tcagcacgcg
     4861 caggctgtag tcggtgaatg cggtgagttt catgggagtt cccgggcagg ccgtgccagg
     4921 ttcagacgcg catctggaaa tcgatgacga tatcccccgg ctcgcgccgc acatattcga
     4981 ttccgatggt gtcgccgaac gcctgctgca gttgcgcgag cagcggcagc gggtcgtggt
     5041 cgttgacgaa gcgcatggtt tcgccagcat tcagactttg caaggcgccg aagatcgccg
     5101 cgtggcggaa ccgcttggcc acgccacgag cgtcgaaggg atgcacgttg ctgaagatcg
     5161 ggatggtggt catattacag gctttcaaca gatgaaacga aggcccggat aaagctgcgc
     5221 taaggagaaa acgaagagcc ggcgaagccg gcccttcgga cctgggtcag gaatgcgctg
     5281 tgacggctcc tgctggagtg cgcagccccg ccgggcggcg cagcagacgc agcgcgtacc
     5341 aggtgagcat caccgcgccg cgccagagcc catgattgct tttcatcgaa agctttcctg
     5401 caataaaatg tccttgaatc ggttctgttt cactcgccgc cgcaggagcc gcagcaggag
     5461 ccactgctgc catgggcatc cgccagcttg gtgatggcaa cgcgccaggt gtcggggccc
     5521 tgctccaggt attcccagcc gaacttgccg gggtactggg cctggaacac attgcgcagc
     5581 ggcttgggat cgtggtcgtt gaccagttcc agggccttgc cggccgcgag gctgccgaag
     5641 gtggagaaga tcagggaatg gcgctgctcg caggcgagtt ggcgaacgtc gatccgggtg
     5701 gcggttgcag tgttcatggg gactccgttg aggctggggg tttaaaggtg tatttcagat
     5761 acacctatcc tatcgacccg cccttgcgcg cacttgatct ggatcaacct cagcgctgac
     5821 acatccggca gcgcgccgga aattcagcga cgcttggcgc ccatgaccag cccatcccac
     5881 ttcgagctgt tgctgcaagc cgcggcgtcc cagcccgagc cgcaacggct cctgttcgtt
     5941 tttgccgagg ccgaattgcc cgccgatgcc agccccgccc aacgcgcgcg cttcgaggcc
     6001 ggccagggtg gcgccctcac gccgctggcc tgcgtggaca aggcggtgga cgacctcgtc
     6061 agcttcgaag cgctggtggc cgaatcgcgc cacgccagtc ccccatggca agtcgtattc
     6121 attgcgggcc tgtccggcca gggtggccgg ccgccttcat cggtgcaagt ggacagtgcc
     6181 ttgcaggcga tggtcgaaaa cgttcgtgcc ggccgcttcg gcggcctgct cgcctttggc
     6241 ccggacggcg agccgctgtc cttttcctga ccattgcgtg cgccactcga gagccttacg
     6301 gtctctccgg caagcgcccg ccatcggcgg ccaggcccag cacggcggcc agcagcaaag
     6361 ccgcggtcac gttgtgcgcg agcgccatgg gcaagggcag gcccccggtc acgagcagca
     6421 cgcccagagc ggcttgcacg accagcagca ggatgaccgc ggcacccgcg cggcggccac
     6481 ttcgccatgc agcgacgccc agcggcaaca gcacggccgc caccagcacg gcgcccgcgc
     6541 gatgcaggcc atgcaccagc gccccggccg gattgacggg gtccgcgaca tcgagcggcg
     6601 tttcatgcca cgggttcagg agttgccagg agccgccggc cacgtcgcac gccagaagtt
     6661 gcgggcagct gagtcccgca tgacccgcgc ttaccagccc gcccaggatg atctgcgcca
     6721 cgagcagcgc caggccaacg cgggcccagc gggtcaggct gtgcggtgcc gcgatagcac
     6781 cgggatggtg gtgcgtggat tcagccagcc gccagctcaa ggcaaacatc gcgaaacccg
     6841 cgagcaggtt gcccagcatg acggccggca cgcgcgcatc cgtggtccag cgtcccagga
     6901 tcgcgaggaa caaggcaagg gccagcaggc cgagggccat gcgcccttcg cgccacagga
     6961 ccggcacctt ggacagcgtc atcatgacca gtgcgatcac gagcagcagc gccgctacgg
     7021 cgaccacgcg gtgcgtgata cgcgccaccg ccaccgcgcc ttccgtcggc ggtgcggcgc
     7081 gttgaccggc cgcttccagg ctctgcgcgt agcattgcgg ccagggctgg cagccaagcc
     7141 cggcctggga caagcgaatg aatgcgctca ggctcgtgat agccagtacc agcgccgcac
     7201 aggccaatgc caattttcga attgtgggta ttcgggcgtc ggctccagcc atgtttcgca
     7261 tgttagggcc tgttcaagtt tgaacaggcc ctggtccgtg ccgggtcggg tattccccat
     7321 aagaggcatt ttcaatacac cttcttgagc cagatcaaag gcacgtcatc agtgtgccga
     7381 caccatccgc aaaccccgcc ccgttccaac aaggagacaa catgagtgaa gaagtcgagg
     7441 acaccggccc cgtgccattg atgcagcagc tgctggacaa cccgttcctg ctgctgttcc
     7501 tgggcgtatt gatccccatg gtggtctaca ccctgtgggg cgtcatcgac atcctgacca
     7561 ttccgctggc caagtaggga gatcctcatg agcgcaattc ttccgccggc ggaacggctg
     7621 tggtggaaac acccccttga ccgcgtcgag ggcacctgga tcgccatcgc cttcgcctgg
     7681 tgcatggtca tgtttttcat gatgatctac tggcacgcgt acggcaaaca gaacctctcg
     7741 accgaaacct acaagaccac gcccgaccag ttctccgcca aggcgcaggc ggtggtggac
     7801 aaatacacgg tgcgcaccga aaccgcggag aagatcccgg tggtcgctcc gccggaaggc
     7861 agcgacgttt acctggtggc gcggctgtgg agctggtggc ccatcctcga gctggaaaag
     7921 aacaagacct acaagctgca cctgacggcg atggactaca accacggctt ctcgctgcag
     7981 ccggccaaca tcaacatcca ggtggtgccc ggctatgaac acgtgatcaa ggtcaccccg
     8041 aaccagtcgg gcaccttctc gatcgtctgc aacgaatact gcgggatcaa ccatcactcg
     8101 atggtcggcc gcatctacgt gaagtaaggg ggccgacatg tccgcgacac tcccgatccg
     8161 aaccacgccc ccgcacgcca cttcctaccg cacctgcccc aggtccgggc tgcagttcga
     8221 gctgcaggcc gaacggctga tgatcgccaa cgccgtgatc gcagtcgtga tgctgctggt
     8281 tggcggcgtg ctggccatcg gcgtggtgct cacgcgctgg cccgccgtcc actggctggc
     8341 cgccgatacc ttctacatgg tgctcaccgc gcacggcatc gacatgctga tcttctggat
     8401 catcttcttc gaaattgccg tcctgtactt ctgctcgtcc acgctgctca gatgccgcat
     8461 cgccacgcca aatctcgcct ggctggcgct cgccctgatg gtgatcggcg ccgtgaccaa
     8521 caacgtcgcg gtgttccagg gcggctccag cgtgatgatg acgtcctacg tgccgatgat
     8581 ggcctcgccc gcgttctacc tgggcctgat cctgtttgcc gtcggcgcgc tgatcggctg
     8641 cttcgtgttc ttcggaacgc tggtgattgc caagcgcgac aagacgtacc agggctcggt
     8701 gccgctggtg accttcggcg ccatcacggc cgcgatcatc gcggtattca ccatcgcctc
     8761 gggcgcgatc atcctgatcc ccaccttcct gatgtcgatc ggcgtcatcc agtcggttga
     8821 cccgctggtc taccgcacga tctggtgggc gttcgggcac tcttcgcagc aaatcaacgt
     8881 cgcggcccat atctcgatct ggtacgccgt cgcggcaatc gctttcggcg cgaagccgat
     8941 gtcggagcgc gtgagccgcg gctccttcct gctgtacatc ctgttcctgc agctcgcgag
     9001 cgcgcaccac ctgctggccg acccgggcct ctcgacaggc tggaagatcg tcaacaccag
     9061 ctacttcatg tatttcgcgg tgctggcgtc gatgatccac gggcttacca ttcccggcgc
     9121 catcgaggtc gcgcaacggg ccaagggcta caacaagggg ctgttcgaat ggcttcgcaa
     9181 ggcaccctgg ggcaacccgg tgttctccgg catgttcatc tcgctgatcg gcttcggctt
     9241 cctgggcggc atctcgggcg tgatgatggg cacggagcag ctcaacatgc tgatccacaa
     9301 caccatctat gtgccgggcc acttccacgc caccgtggtg attggtacga ccttgacgtt
     9361 catggcgctg acctacttcc tgatcccggt gctgttccgc cgcgaaatga tcaatccggg
     9421 cctggcgaag ctgcagccgt atatcttcgg cctgtcgatg tacttcttcg tcctggtgat
     9481 gatgggggcg ggcacgctcg gcgtctcgcg ccgccactgg gacatggcgc tcaccggcgc
     9541 cgccctgggc tacgagtggc cgggcgcggc gtacctgatg atggccctgg tcggcattgc
     9601 cggcatggcc gccatcatcg gcggcggcat ctatatctac atcaccgtcg gctcgctctt
     9661 gtggggcaag aagctcgaca ccggtgccgt cagcccgatg ttcacgccga tcccgcctac
     9721 cgcgaagacg gctgtcgcgc agtcgtacgg ctccgccggt ttcgcggcac ccggcacctt
     9781 catgctggcg atgctgttcc tggtggcgtt cgtgctgtac tacttcatca actggaagta
     9841 cctcgggcag ctgtggggcc tgagctaatc cgcacaacgg catgaatacg accactctgg
     9901 ggggcacgcg tcggcgcacc gcgcaagtcg cgcgcgacgt gctctccctg ttcaagctgc
     9961 gcatcggcgt gctgatcatg gtcacggcgc tggtaggcct ggcggtgacg ccgggcgccg
    10021 cgctggggct cggccaggtg ctggtgctgg cgctgtcggt gctggtcgca tcggctgccg
    10081 ccggcgcctt caaccagtat gtcgagcacg acagcgacgc gttgatggcg cgcacgcggc
    10141 gccgcgcctt cgtgacgggc gcgctgtccc accatccggc atggctgctg ctgatcggcg
    10201 tgctgctcgc cggctcggtg gcggcggcgt ggttcacgct gaacgcgatg gcggcgatgt
    10261 acgtcttcct gggtgcgttc ttctacgcgg tggtgtacac ggtctggctc aagcgccgca
    10321 gcgcgctcaa catcgtggtg ggcgggttgg ccggcagctt cgccgtgctg gccggcgcgg
    10381 ccgccgcgga ccccacgccc ggccccctgc cgctgctgct ggcgctggtg ctgttcctgt
    10441 ggacgccgcc gcacttctgg agcctggcca ttgccaaccg cgacgactac gccgccgcgg
    10501 gcgtgccgat gctgccggtc gtggtcggga tcgaacgcgc cgcgcgcatc gtgttcgcaa
    10561 gcaccgcggc cctggtcgtg gcatcgctcc tgccgctggc cttcggcgcg ggcccgatct
    10621 acgccgtggg cgccatcggc ggcggctttc actttctgcg caagtcgtgg ctgctggccc
    10681 ggcggcccga ccgcgccagc gcgatgggct cgttcttcgc ttccctggtc cagctgagcg
    10741 cattgctggt cgccgcgacc ctggacggcc tgctccgctg aaatgaaaac ccacccccga
    10801 agcgccttcg gcgcctcccc ctcaaggggg cgccaccagc ggcccggcga agccggttcc
    10861 gcggtggccc ccgcactgac cgcaaggttt catgtgctgc gggtggcgct aagcgcaatg
    10921 gatcgctgaa aggtttcagg caggccttat gcgcttcccc cgactcgccg gtgcggccca
    10981 gctcgcggca acgctgtcgc tggccggcgc cttgctgcag ccgctggcgg cgccggcgca
    11041 ggaggccgcg cgcgttcccg ggctcgacca gcaggccgcg atcaaggcca gccaggcggt
    11101 gctgggccaa agcatcggcg actacacctt gctggaccgc gaaggccgtc cggtgcggct
    11161 ggcctcctac cgtggcaagc cgctgctcgt gagcttcatc tacaccggct gcttccaggt
    11221 gtgcccgacc gccacgcgct cgctggacga ggccgtgcgc gccctgcagg gccgcttcgg
    11281 cgacaaccag ttcaacgtcg tgagcatcgg cttcaaccag ccggccgact cgccgcaggc
    11341 gcttaaggcc ttcgcggcgc aacaccgcat ttcgcggccg aactgggatt tcctcagccc
    11401 gccctcgccc atcgtcgggc cgctgacgcg agacttcggc ttcctctacc aggccacccc
    11461 ggcgggattc gaccatgtgc tgcaggtctc gctggtcgac gcccaggggc ggatcgcgcg
    11521 gcaggtctac ggcgaccgcg tagcggccga cgccctgggc gagccgttga aacagatgct
    11581 gatcggcgcg ccgctgccgc agggcatccg gctggacgac atcatcgacc aggtccggct
    11641 gctgtgcacg gtgtacgacc cgaaaacggg cacttaccgc gtggactaca gtctggccat
    11701 cgaattcgcc ggcggactga ccttcatcat cgccgtagcc ctgtacatgc tcaacgagtg
    11761 gcgctcgcgg cgcagggcac gccgccgggc gcagggcgcc tgagggcgct gtctcgccgc
    11821 gcagtttcag gcccggacag atcgacatgg caaagctcgc acccgccggc cccagcgtgc
    11881 tccacctcgc gcagcccacg ccaccggagc ggcccggtgc gaaagcggcc tatcgccggc
    11941 tcgaaaacgc attcgacgcg gctttcggcg gcgctttgaa tccgctgaag cacctgggcg
    12001 cgctcgcctt cctgctgttc tggctgctcg ccgtcaccgg aatcgtgctg tacgtcgtcc
    12061 tcgatacttc cgcacatggg gcttatcgct ccatcgacga actctcgcgc gatccctgga
    12121 gcttcggcag cgcgctgcgc ggcttgcacc gctacgccgc cgacgctttt gtgttcgccg
    12181 cgctggccca tctcgcgcgc gaatggctgc tcggccgcta cagcggcttt cgcgccttct
    12241 cctggctcac gggcgtgccg ctgttgccgc tcgcctatgt ctgcgccatc ggcggcttct
    12301 ggctgaactg ggaccggctc ggccagttct ccgccatcgc cacggccgaa tggctcgatg
    12361 ccctgccctt cctcgcctca cccctggcac gaaatttcct cggcggcgcc gtgaacgacc
    12421 ggctgttctc gctgttcgtg ttcgtgcatc tgggcgtgcc cttgttgctg gtgttcgggc
    12481 tgtggttcca catccagcgc atcagccggg ccgaggtgtt cccgccgcgg gcgctggcgc
    12541 tgggcaccac cgccgctttg ctggcgctcg cgctggcact gccggtccgc agccagggcc
    12601 cggccgacct cgccgtcgtc ccgggacggc tgtccctgga ctggttcctg ctcttcatcc
    12661 acccgctgac cggcgcgacc tcggctgctt tcgtatgggc actgctcacc gcggcgatcg
    12721 tcggcctgtt cgcgctgccg ttcttgcgcc gccgcgccgc gccacccgtg gcgcaggtgg
    12781 accccgcgaa ctgcaatggc tgccggcgct gcgccgacga ttgcccgtat ggcgccgtga
    12841 cgatggtccc gcatccgaac gggcggcccg gcgcgcagct ggcccaggtg aaggccgagc
    12901 tgtgcgcgag ctgcggcatc tgcgtcggcg cctgcccctc ggccacgccg tttcgcaaag
    12961 gcgaaacgct cgtgaccggc atcgacatgc cgcaatggcc catcgatgat ctgcgccgtc
    13021 gcctggtccg ggggcttgcg gccatgcgcg ccgagcggcg cctggtcgtc ttcggttgcg
    13081 accagggcgc gcgcgtccag gcgctggccg cacccgacgt gctgccgttc agcctggtct
    13141 gcgccggcat gctgccgccg tcgttcgtcg agtacgcatt gcgcgccggt gccgacggcg
    13201 tgctcgtcgc gggctgccgc gagggtggat gcgaatttcg tctgggccag cgctggacgg
    13261 cgcagcgcct gctcggcctg cgcgagccgc gcctgcgcgc cgcgatgacg gccggacggt
    13321 gggccacctt atgggccgac gccggtgacg aggccgcgct gcaagcggcc ctcgacaagc
    13381 tgcgccgcga agccggcatg gccgcaccgg cgacgctcgc ggaggccccc gcatgaagcg
    13441 cgtgatgtcc tgggccggac aggtcctgct gtatggcctg ttcgccgtct ttgtcggggt
    13501 gttctcgagt tggccggcct acgaggcgtt gccgccggac caggcgctgg tcaaggtgag
    13561 cttcattcat catggccaac gggtcgccga atgccggccg tacacggccg aggaactggc
    13621 caggacggca cccaacatgc gcacgccgat gaaatgcgaa cgcgaacgct cccccgtcac
    13681 catcgaggtc gacctggacg gggccaccgt gtaccgccgg gtggccgcgc cttcggggct
    13741 atcgcgcgac ggcgcctcgt cggtctacca ccgcatcgcc gtgcccgccg gcgagcaccg
    13801 cattgccgtg cggctgaagg acagcgcttc ggcacgcggc ttcacgcaca cccgcgacgc
    13861 gacgttgacg ctcaagccgg cccaggtgct ggtgatcgat ttcgacgcgg aaaaaggagg
    13921 aatcaccctg tcatgagcgc cgttcccact tccctggccg gaccgcttgc ccagcgaccc
    13981 ttgcccgcgg tccagtccgc cactgactac agcttcaccg tgccggccga tgcgcgcctg
    14041 gcgctcgcac gcggctggct gtggctcggc atggcggcac tgataggctc gggcctgttc
    14101 tcggtcctgc tggtgctctc ccgcacaccc ggcgtcaacg aactgctgcc ggtggcggat
    14161 ttcttccggg tcgcgctcgt ggtgcacgtg gacctgtcgg tgctggtctg gttcatggcg
    14221 ctcgcgggca tgctgtggac gctcaacgga tccgcgcgcc gcatgggctg gggctggtgc
    14281 ggcctcgcgc tggcggcggc cggtgcggcg gcgatgtcga tcgcgccgtt cagcggcccc
    14341 ggcgaggcca tcatggccaa ctacatcccc gtgctcaccg cgcccctgtt catgatcggc
    14401 ctgctggcat tcgcggccgg cgtggccgtg ctggtgctgc gcagcctcgc caacgcgccc
    14461 cggctgggac tggagttcga cgggcgcggc gcgctgcgct tcggcctgaa cgccgccgct
    14521 gtcgcgaccg cggtcgccct gctctcattc ggctggtcct atgcggtgct tcccggagac
    14581 ctcagcggca aggcctatta cgagatcctg ttctggggcg gcggccatgc cctgcaattc
    14641 acctggacgc tgttgatgct ggtggcctgg ctctggctcg cgggtgcctg cggcgcgcgc
    14701 gtgccgctca gcccgcgtgt cacgctgctg atgttcgccc tcgccctcgt gagcgtgttc
    14761 gtcacgcctt atgcctatct cgcgtacgac atcgcgtcgg tggagcaccg cgcattgctg
    14821 acctgggcca tgcgccttgg gggtggggtc gccatcctgc ccattggcct ggcggtggtg
    14881 ttcggcctgt ggaacgcgcc gccgctcgac gcagcggccc ggcctttgcg ggcggccctg
    14941 ctgtcgtcgg tcctgctgtt cgccgcgggt ggcgtcatcg gcattttcat cagcggcagc
    15001 aatgtgcgca tcccggcgca ctaccacggc tgcatcgtcg gcgtgaccct ggcgctgatg
    15061 ggactggttt tcaagctgct gcctgcgctc ggttacggcg cgccgcgggg acgcctggcc
    15121 accagccagc cctggctgta cgcggtcggc cagttgatgc acatcgtcgg cctcgtgtgg
    15181 tcgggcggct acggcgtgca gcgcaaggtg gccggcgccg agcaggtgct gcgcagtccg
    15241 gccgaaatcg cggggatggg cctgatgggc ctgggcggcg ccatcgccat cgtcggcgga
    15301 ctgctgttcg tcgtggtcgt gctgcgcgcc atgcgccacg cccgcgtgcc ggccggcagc
    15361 ttgcaagcgg cgcggtaatg aagcggttgc aggcgttgct gcagtggctg ttcatgcgcc
    15421 tggaagcgct gttcaacgcc gcgttcggcg accgcctcaa ccccctgtac cacctcggcg
    15481 cgatcacttt cttcctgttc tgggtggtcg gcgcgagcgg gctgtacctg tacgtgtttt
    15541 ttgaaaccgg ggtggccgaa gcctattcgt cggtcgaggc gctgacgcat cgccaatggt
    15601 tcgccggcgg catcctgcgc agcgtccatc gctacgcctc ggacgcgatg gtggtcacga
    15661 tgggcgtcca catgctgcgc catttcgcgt tcgaccggtt ccatggcttt cgctggttct
    15721 cgtggatcac gggggtgtcg ctgatctggc tggtctacat ctcgggcatc aacggctaca
    15781 tgctgccgtg ggaccggctg gcgcagttcg tgatcgtcac cagcttcgag tggctggact
    15841 ggctgccaac cttcggcggc acgctgatgc gcaacttcgt ccacgcgaac agtgtcggcg
    15901 accgcttctt ttcgctgctt tcgttcatgc acatcggcct gccgctgatc gtgctgctgc
    15961 tgatgtgggt gcatgtgcag cgcgtgccca aggctcgcac gacgccgccg cggccgatcg
    16021 tggcgagcct gctggcgacg ctgctggtgc tgtcgctggt gcggccggcg ctgagccagg
    16081 gcggcgcggc ggacctgggc accgcggtat cgagcctgca gctcgactgg ttctacctcg
    16141 cgctgtttcc gctgctcgtg gcgtggccgc tgggccaggt ctgggcattg atggcgggcg
    16201 gcaccgcgct actcgtggcg ctgccgttgt ggccggagct gttccgccgc aatcgccatc
    16261 gcgaatacca gctcgtcgta cacagcgccg acggcgccgc gacccagctg cgcctgcggg
    16321 atggcgaaac actgctggag gccggcttgc gcgagggcct tgcgcttccc tacgaatgcc
    16381 gcaatggcgg gtgtggcgta tgcctttgca gcgtcgagca cggcgccgtg tcgcatcgcc
    16441 cctaccagcg cagcgccctg cccgatgcat tgaaggcaca gggcaaggcg ctgatgtgtt
    16501 gcgcggttcc gatgggcgat atggtgatcg aggtcgaagg gctcgcgcac gggggcgccg
    16561 cgacggcaaa gctgcacacc gggcgagtgg tcgcactcga acgactcgcc caagacgtga
    16621 tgcgggtgct gatcgcactc ccggaaggcc agcgcgtcgc tttcaccgcg gggcagtaca
    16681 tcaacgtcct gctggacgat ggcgagcggc gggcgttttc gttcgccaat gcgccgcatg
    16741 agaacgagct cgtcgaattg catgtgaggc gcatccccgg cggccgcttc accggccatg
    16801 tgttcgaggc catgcgggtg ggcgacacac tgcggttcga gggaccgctg ggcagcttca
    16861 cactgaacga gagcgcccgc ccgatcctgt ttgtcgcggg tgcgaccggc ttcgcgccgg
    16921 tgaagagcat catcgaagac gcgttccacc gcggcctgca ccgtcccatg cgcctgtatt
    16981 ggggtgtgcg gcggcgcgag gacctctacc tgctcgatct cgcggaacgc tggcagcgcg
    17041 agcacgagaa cttcctcgtg gtgcccgtgc tctcccaccc ggaagccggc gacgagtggc
    17101 acggccgcac gggcctcgtg cacgaagcca tgctcgccga tttcggcgac ctcgtcggct
    17161 acgaggtcta cgtctgcggt tctctgcgca tggtggaagt ggcggtgccc gcattcatcg
    17221 cgcagggcct gaacgaggac gcttgctttt cggacgcttt cctgccgtcg gcggaagtca
    17281 gtcaacccgc cgggttggtt gcttgagctc cgttcagagc ccgagcccct gcaggatctc
    17341 gtccatgcgg gcctggacag ccttgtccat ggcgatcggg cggccccatt cgcgcggggt
    17401 ttcgccgggc cacttgttgg tggcgtccag gcccatcttg ctgcccaggc cgctgaccgg
    17461 cgacgcgaaa tccaggtagt cgatcggcgt gttctccacc agcatggtgt cgcgcgccgg
    17521 gtccatgcgc gtggtcatcg cccagatcac ctcgcgccag tcgcgcacgt cgacgtcctc
    17581 atcgaccacc acgatgaact tggtgtacat gaactgccgc aggtggctcc acacgcccat
    17641 catcacgcgc cgcgcatggc ccggataggc cttgcggatc tgcaccaccg ccatgcggta
    17701 gctgcagcct tcgggcggca ggtagaaatc ggtgatctcg ggaaactgcc gctgcagcag
    17761 cgggatgaac agctcgttca gcgccatggc cagcaccgcg ggctcgtccg gcggcttgcc
    17821 ggtgtaggtg gagtggtaga tcgggtcgcg ccgcatcgtg atgcggtcga tcgtgaatac
    17881 cggaaaccag gcctgctcgt tgtagtagcc ggtgtggtcg ccgaacggcc cctcgagcgc
    17941 gtgttcatag ccgctgggat ggctggcatc gggctggata tggccttcca gcacgatctc
    18001 ggccgtggcc ggcacctgca ggtcgatgcc caccgccttc gcgatttcgg accgccctcc
    18061 gcgcagcagc ccggcgaact ggtactccga caggctgtcg ggcacggggg tgacggcgcc
    18121 cagcagcgtg gcgggatcgg cgccgagcgc caccgcgacc gggtagggct caccgggatg
    18181 ggcgatgccg tgttcccgga agtcctgcgc gccgccgcgg tgcgcgagcc agcgcatgat
    18241 gagcttgttg cgcgagatca cctgctgccg gtagatgccc aggttctgtc gccccttgtt
    18301 gggaccgcgc gtgacgacca ggccccaggt gaccagcggc gccacgtcgc cgggccagca
    18361 atgctgcacc ggcagccgtg ccatgtccac gtcggcgcct tcccagacca cctcctggca
    18421 gggcgcgccg ctcaccacgc gcggcgccat gtgccacaag gtcttgagca ggcccgagag
    18481 gcccatcatc tccttgaagc ccttgggcgc ttccggctcc ttgagcgcgg ccagcgccgc
    18541 gccgaacggc cgcagctcgc gcaggtcgtt cacgcccatg gcgcgcgcca cgcgcgccgg
    18601 cgttccaaac aggttgccca gcaccggtat cgtgtggccg gtgggccgct cgaacagcac
    18661 ggccggtccg ccggcgcgca gcacgcggtc gcacagcgcc gtcatctcca ggtgcggcga
    18721 caccggctgg ctcacacgcc gcagctcgcc caggccttcc agctgggcca tgaaatcgcg
    18781 caggtcgcgg taagccattg cgtcccgctc agaggctcgc gccggcgggc gaaagccctt
    18841 cccagcgcgg cgccaggttg tgcggcagct cgagcaggtc gagcacgcgg gccacgccgg
    18901 cgtccacgat ctcggccacc gattgcggcc gcaggtagaa ggccggcatg ggcgggcaga
    18961 cgatggcgcc catctcggtc gcgctgacca tgttgcgcag gtgcgccagg ttcagcggcg
    19021 attcgcgcac catgagcacc aggcgccgcc gctctttgag catgacgtcg gcgcagcgcg
    19081 tgatgagatt gtcccccagg ccatgcgcca ccgcgcccag cgtgcgcatc gagcaaggcg
    19141 cgatcaccat gccgctgctg cggaacgaac cgctggcgat ggccgcgccc acgtcgcgca
    19201 cgtcgtgcac gcgatgcgcc agcgcttcgg ccgatccgcg gtccaggccc tgttcctgct
    19261 ggagattgcg ccagcccgcg tccgatacga ccaggtgtgt ttcgacgccg tcgatgcccc
    19321 gcagcacctg cagcagccgc acgccgtaga cggcgccgct ggcgcccgag atcgcgacga
    19381 tcatgcgaat cagcgcgggc ggcgcggtct tcatcgccta cttgcccgat ccgtgatggc
    19441 gtggcccgat gtccgaggaa tgcgtccggg gcgccaggcg agccagcacg cggtgcggcg
    19501 cccagcgttg cagttcgaac accggcagtt ccagcgcatc gagcgtgttc ttgaccacca
    19561 ggccgagttc cgtgtcgcct tccatcgaca ggcggcggct gaaaaagagc gtgtccgggt
    19621 cttcgcggcg ctgcgccagc tgcaggaagt cataggcgct ggcgccgatc gtgagatccg
    19681 cctcgttccc gccggcacag gcggcgaagc cctggccggt ccaggtgaaa tcgaaagtca
    19741 ggcgggcgtc gcgaacgtgg atgcgcagtt tcttgcccgc aagcagctgg cgcacatcgt
    19801 cctgcaactg gtccgccaat cccaggttga gtgccgtgga cagcagcacc gatccggggt
    19861 aggccggcag gcgcgccagc agcataccga ggggccgcgg cactgcgtgg gaaggagtgt
    19921 agttcatagg tttccaatgg cgtcccgcgc cacccgtgaa gtgtgagagc cgaggctccg
    19981 ttcgcgggcc accgcggaac cggctttgcc gggccgctgg tggcgcccct tgaggggagg
    20041 cgccgaaggc gcttcggggt ggtcttccat ttcaggccac gtggagcagg acggcgaaaa
    20101 aatgacaggc gctgccgccc agcacgaaca ggtgccagat cccgtggcca tggcgcagct
    20161 tgtggtcggt ggcgtagaac aggataccga tcgtgtacat ggcgccgccg gccagcagcc
    20221 aggcgaaacc cgccggcttg agcgcctgcc acagcggcag caccgccacc agcgccagcc
    20281 atcccatcac gatatagatg acgagcgaga gcacgcgcga tcccttggcc agccagatct
    20341 cctgcgcgat gcccagcagc gccagcgtcc acacggcgcc gaacagcgac cagccccagg
    20401 ggccgcgcag cgaaacgagc gtaaaggggg tgtagctgcc ggcgatcagc aggtagatgg
    20461 agcagtggtc gatcttgcgc agcacgttct tggcccggcc gcgcacgctg tggtacagcg
    20521 tcgagaaaac gtacagcgcc accagcatcg cgccgtagac gctgaagctc acgatcttcc
    20581 aggggtctcc cagttgcgcc gccagcacga ccagcagggc agtaccgccg acagccagcc
    20641 cagccccgac gaggtggctg atgctgttga atcgctcgcc ggcatacatc aggcggcctg
    20701 gccctgctcg agcccgggcc ggccatgcca gtagccgttg caggcttcgc cgggcatcag
    20761 gggtgcgagg cgggccagcg cttcccgcgg cgccagcgcc ttcttgcggg cctggtcgaa
    20821 cagcgcgatc acgtccgggg tatcgtttgc ctgcgggctg atccgcagca tgtccacgcc
    20881 cagcgcgcgc atgtcctcga tctcgtcgat caggttgtag atgcgcgccg actgcgtctg
    20941 ggtgccgttg agcaccagga actcctcgct ctcgcgcgtc ttgagcagca ggccgtcggg
    21001 atgctccatg cagctgaagc ggcaatcgtc cttgggcagg ttgcggtggc gcgcggtgaa
    21061 gcagcgcgcc gaaaaggcca gcggcatccg gccgtaggcg aacacctcgg tctgcagccc
    21121 cgcggggcgg gccggctgca acagcgcgag gtcgtcgcgc ttcatctcca gcgggatcac
    21181 ccagcggctg gcgcccagtt tggccatcca ctccagcgtc ggcaggttgt acaggttcag
    21241 gtgcgggccg gcgacgaagg cgcctctgcc cgcaaggcga tgcaccgcgc ccatgtcatt
    21301 ggcttccacc aggtactcgc cgttggcggc gatcttgtgc atgctgctca cgtcgctgcc
    21361 ggactccagc agcacctggg tggagagcac cacgtccttg ccggcctggc gcagccgccc
    21421 cgctatttcg agccagtcgg ccaggcgcag ttcatggcgc cgcgagcaca cggtctcgcc
    21481 gaggtagacc acatcgaccg gttgatcggc cagttggcga tagaattcaa acactttatc
    21541 gcgcggccag aagaactgca ccgcgcccat ggataatttc atcgttctac tcctgcacgc
    21601 tt
//
LOCUS       DASWIY010000295        18224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1685482, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000295 DASWIY010000000
VERSION     DASWIY010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 18224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18224
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685482"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..294
                     /locus_tag="VGA59_10810"
     CDS             118..294
                     /locus_tag="VGA59_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720212.1"
                     /translation="MPRLHQHGCRRRNGFLATEVFHLWHPENSRAGEAGKRERVVQRM
                     QGSGTAPIVTVLNE"
     gene            316..1596
                     /gene="tolB"
                     /locus_tag="VGA59_10815"
     CDS             316..1596
                     /gene="tolB"
                     /locus_tag="VGA59_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX9720213.1"
                     /translation="MNNPVLWRRRQLVALLGAAALPAFAQFRVEVTGVGLTQLPIAIA
                     PFRGEGQAPQKIAAIVQADLERSGQFRAVDAAGAALDEGSRPDLTPWRQKGADSLAAG
                     SMARLADGRWDVRFRLWDVVRGQDLGGQSYAVTVGDLRLAAHRIADFIYEKLTGEKGV
                     FSTRIAYVTKTANRYNLWVADSDGENAQSALASAEPIISPAWSASGAQLAYVSFESRK
                     PVVYVHDVATGRRRLIANFRGSNSAPAWSPDGRTLAVTLSRDGGSQLYTINANGGEPR
                     RLTQSAGIDTEPLYAPDGRNIYFVSDRGGAPQIYRMPSSGGSPERVTFTGSYNISPAL
                     SPDGRWLAYISRISGAFKLQVMELGSGTVHSLTDTNADESPSFAPNGRLIVYATRQNG
                     REALMTTTLDGKIKARLSGQSGDIREPDWGPFQK"
     gene            1617..2147
                     /gene="pal"
                     /locus_tag="VGA59_10820"
     CDS             1617..2147
                     /gene="pal"
                     /locus_tag="VGA59_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX9720214.1"
                     /translation="MIKRMFLAVAVALAVAGCSSVPLDDVPVEDKSAGAVTGQPGGPG
                     SGTQSSVAPVAADPAIGTVVGPANVARIIYFDYDSYVIKPQFQSLLDGHARFLRANNG
                     RRISIEGHTDERGGREYNLALGQRRSEAVRQALGLLGVPDRQVEAVSFGKEKPVAMGS
                     DEAAWSQNRRAEIVYR"
     gene            2147..2953
                     /gene="ybgF"
                     /locus_tag="VGA59_10825"
     CDS             2147..2953
                     /gene="ybgF"
                     /locus_tag="VGA59_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX9720215.1"
                     /translation="MSTNTRHPGFHWRAAVASGLAMLCLAASSVASAALFEDDEARRA
                     ILELRQRVDAQRLAGDRQAEELRRAYEENGQMRRSLLELQSQIEALRGEVARLRGQDE
                     QLVRDLADVQRRQKDLVQGVDERLRKVEPSQVSVDGREFIADPAEARDFEAALAIFRR
                     GEFPAAQAAFAEFIKRYPQSGFKPTAMYWLGNAQYANQDYRGAIGTFRALLAQSPDHP
                     RAAEAVLSIANSQLGLKDTPGARKTMEELIKAYPQSEAAVAAKERLARFR"
     gene            2976..3764
                     /locus_tag="VGA59_10830"
     CDS             2976..3764
                     /locus_tag="VGA59_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEX9720216.1"
                     /translation="MTHLDEAADLQRRFGGLERLYGMSGAAAIRASHVAVVGIGGVGS
                     WAAEALARSGVGELTLVDLDHIAESNINRQIHALAGTLGQAKVRAMAERIAQINPRCR
                     VHGIEEFVEPDNWPQLLPDGVVAVIDACDQVKAKVAMAAWSLQTRTLFVAAGAAGGKR
                     LAHKVDLDDLANTTHDPLLAQVRTRLRKLHGAPREGRKIGVACVFSREAVAPPDASCA
                     IESDGSLNCHGYGSVVSVTATFGQCAAGWALDRLATAKTGIGTI"
     gene            3856..3931
                     /locus_tag="VGA59_10835"
     tRNA            3856..3931
                     /locus_tag="VGA59_10835"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3889..3891,aa:Lys,seq:ttt)
     gene            4330..4632
                     /locus_tag="VGA59_10840"
     CDS             4330..4632
                     /locus_tag="VGA59_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720217.1"
                     /translation="MRNFVVKLAAWGRVCKDLDDARLRLAHAETAQPPGQRARATEAH
                     VRRLERARDQALGELRIAAEAYMATVRQQSASTPVENPRSRLMDLDLDLGQGTAWA"
     gene            complement(4816..5331)
                     /locus_tag="VGA59_10845"
     CDS             complement(4816..5331)
                     /locus_tag="VGA59_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720218.1"
                     /translation="MATSLQLADGNAMLMDAVRRSRKLLNRRALVGAAASTVPMPGLD
                     WAVDAALLSRLVPAINAEFGLTPAQLDQLPGHKREQVQKALAMVGSVMVGKFITRDLV
                     IRMAQTVGRRLTVKQAAKYVPLAGQAVSAIMGYTAIRYLGEEHIKDCVRVAQEAQLLL
                     PAPEKKRLTHR"
     gene            complement(5374..7485)
                     /locus_tag="VGA59_10850"
     CDS             complement(5374..7485)
                     /locus_tag="VGA59_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9720219.1"
                     /translation="MIVLRNLTLRRSAKVLLDKVSVTINPGEKVGLVGRNGSGKSTLF
                     ALFNGSLHEDSGDFSMPPQWRLAQVAQDMPETGEAATAFVLGGDTRLVALREALARAE
                     ASHDANAHDDHGMEIAHLHSDLADAGEHDAVPRAQSLILGLGFKASELYLPVNSFSGG
                     WRMRLQLARALMCPSDLLLLDEPTNHLDLDALVWLEAWLKRYTGTLVVISHDREFLDA
                     VTSVTLHIERQQLTRYGGNYSAFETLRAQQLELQQSAFSKQKEKIAHLQKFIDRFKAK
                     ATKARQAQSRVKALERMEKIAPVLADADFSFEFKEPANLPNPMLAIRDGVFGYLPSPQ
                     PSPASGRGREHEADRNLRPLPAGEGGGEGTAPKIILRGVSRSVMAGQRIGILGANGQG
                     KSTLVKTIARDIPALGGTITEGKGLNIGYFAQQELDVLRPFDTPLEHMFRLAREPGPN
                     SGESSREQDLRNFLGTFNFAGDMVKQAVGTMSGGEKARLVLAMMVWQRPNLLLLDEPT
                     NHLDLATREALSMALNEFEGTVMLVSHDRALLRAVCDEFWLVGRGQIAPFDGDLDDYQ
                     RYLLEEAKRLREEARAEGAAAAADSRPPEAAPQAAGSGAAWQAVLTPQEQRKQDAQLR
                     QQRATRAKPLKRELEQAEQRMAALHADKAALESTLATAPPPLEIAQAGKRLKAVTDEL
                     RALEERWLALSDDLEAIETAR"
     gene            complement(7482..8717)
                     /locus_tag="VGA59_10855"
     CDS             complement(7482..8717)
                     /locus_tag="VGA59_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEX9720220.1"
                     /translation="MTKDITTRILHADRLGSVEHGAVLKPLHIATAYGYPTAEELTAV
                     FQGRQSGHVYGRQGNPTTAALEVKISLMEDAVGTVCFATGMAAICSAMMALLKKGDHV
                     VASRYLFGNTASWMATLSQNGCEVSLVDATDAANVRQALRPETRMVFVETIANPRTQV
                     ADLEGIGRLCTEHGLLYVVDSTMTTPHLFQPKAVGASLVVHSLSKSICGHGNALGGAI
                     SDTGLYDWNRYPHLLPAYRKGPPSGWGLMQIRKKGLRDIGATLSSEHGHRIAAGAETL
                     SLRMERTCANARALAEWLEAQPLVSAVHYPGLASHPQHARAASLFKDFGALLSFETVE
                     GVDPFQVLDALELVIKSSHLGDNRTSALPVAHTIFWEMGPEQRASMEIADSLIRVSVG
                     IEAQGDLLDDFAQAFAKLK"
     gene            complement(8755..9714)
                     /gene="prmB"
                     /locus_tag="VGA59_10860"
     CDS             complement(8755..9714)
                     /gene="prmB"
                     /locus_tag="VGA59_10860"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX9720221.1"
                     /translation="MTVIELVEASARQLADAGVAFGHGTTNAFDEAAWLVLWQLGLPL
                     DDLDGVADRAVSAQQRGGVLELVAERIRSRKPAAYLTHEAWLQGLPFYVDERSIVPRS
                     FIAELLVDGGIDPWLGERTSRVLDLCTGNGSLAVLAAMAYPEVAVDASDVSADALAVA
                     RINVDKHGLRDRIRLLQSDGLASVPGPYDLILCNPPYVNAQSMAELPAEYRAEPPMAL
                     AGGADGMDFIRALLRHAPAHMSEHAVLVLEIGNERGNFEAAFPRLEVVWLETSAGEDQ
                     VLLVTQDSFQACHCGPGSSPGFQHPQSMDPGSMLAPDSIRGPG"
     gene            complement(9711..10883)
                     /gene="dapE"
                     /locus_tag="VGA59_10865"
     CDS             complement(9711..10883)
                     /gene="dapE"
                     /locus_tag="VGA59_10865"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX9720222.1"
                     /translation="MMAALHLTEQLISRRSVTPEDAHCQQIIAERLQPLGFACETILS
                     GPDTFRVTNLWAKRPGSRELAKTGAGRTVVFAGHTDVVPTGPADQWTTDPFTPSHRDG
                     KLYGRGAADMKTSIAAFVVAVGEFLAARPEPALSIALLLTSDEEGPAVDGTVVVCDRL
                     EDRRERLDYCIVGEPCSVERLGDMIKNGRRGSMSGRLTVKGVQGHIAYPQLAKNPIHL
                     ALPALAELATLEWDWGNEFFQPTSWQISNIHGGTGAGNVIPGTVVVDFNFRFSTESTP
                     EGLQKLTHGVLERHGLDYGVEWVVGGLPYLTTPGELVGAIQDAIRGETGIAARLSTTG
                     GTSDGRFISRICPQVVEFGPVNASIHKIDEHVLVADIEPLKNIYRRTLENLEELLR"
     gene            complement(10880..12037)
                     /locus_tag="VGA59_10870"
     CDS             complement(10880..12037)
                     /locus_tag="VGA59_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX9720223.1"
                     /translation="MSTMERILRLMSEKKASDVYLSAHSPALIRINGQCLPINNQRLP
                     PDAPRALLAEVLPAQRMDELEQTGELNMAHAIDGIGNFRVSAMRQRGTYAAVIRYITT
                     EIPSLPSLSVPMVLADLIMEKRGLLLMVGATGCGKSTTLAAMMDHRNDKVSGHILTIE
                     DPIEYLFTNKKSVVNQREVGSDTESMQTALKNALRQMPDVILIGEIRDRETMSAAIAY
                     AQSGHLCLATMHANNSYQALNRILSFYPVEVRPTMLGDLAAALKSIVSQRLLRTVHGA
                     RTPAVEVLLNTKLVAELIEKGDFSGVKEAMEQSMAEGSQTFEQDIARLIVEGTVDKKE
                     GLAFADSPTNLQWRLQNDFAGKDGAQAEDGASDEDLGDEPSFTEITLDVKH"
     gene            complement(12068..12892)
                     /gene="dapD"
                     /locus_tag="VGA59_10875"
     CDS             complement(12068..12892)
                     /gene="dapD"
                     /locus_tag="VGA59_10875"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX9720224.1"
                     /translation="MTQQLQQIIDNAWENRASLSPTCVPKEVADAVEHVIGEMNNGHL
                     RVATRIGVGEWTVHQWIKKAVLLSFRLRENELMRAGDLGFFDKVPTKFAHLSMQEMVA
                     TGVRVVPPAVARRGSYVGKGAILMPSFVNIGAYVDEGTMVDTWATVGSCAQVGKNVHL
                     SGGVGLGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEENSVISMGVYLGQSTPIYD
                     RAAGTVSYGRIPAGSVVISGSLPKDGGRYSLYAAIIVKRVDAQTRAKTSLNDLLRD"
     gene            complement(12889..14091)
                     /gene="dapC"
                     /locus_tag="VGA59_10880"
     CDS             complement(12889..14091)
                     /gene="dapC"
                     /locus_tag="VGA59_10880"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEX9720225.1"
                     /translation="MNPLLSRLQPYPFERLRQLFVGVTPNPAYRPISLGIGEPKHPTP
                     EFIKQALTASLSGLSSYPLTAGAPHLRDAFRRWLQTRYGLMAVDANTQMLPVNGSREA
                     LFAFAQTVIDPGGKPVVVCPNPFYQIYEGAALLAGAEPFYAASDPARNFAVDWDSVPL
                     ETWSRTQLLYVCSPGNPTGAVMPLAEWEKLFALSDRHGFVIASDECYSEIYFTGEPPL
                     GSMQAAAKLGRADFRNLVSFTSLSKRSNVPGMRSGFVAGDAALMKKFLLYRTYHGSAM
                     SPVVQQASIAAWGDERHVVENRDLYRGKFAEVTPMLARVLDVRLPDAGFYLWAGVPGS
                     DTEFARDLLAQYNVTVLPGSYLARESGGFNPGAGRIRMALVAETAECVEAARRIVQFV
                     QSRNEVNQ"
     gene            14334..17849
                     /gene="smc"
                     /locus_tag="VGA59_10885"
     CDS             14334..17849
                     /gene="smc"
                     /locus_tag="VGA59_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX9720226.1"
                     /translation="MRLNSIKLSGFKSFAEPTNFMLPGQLVGVVGPNGCGKSNIMDAV
                     RWVLGESRASELRGESMQDVIFNGTNTRKPASRSSVELVFDNADHRAGGQWSQFTEIA
                     VKRVLTRDGTSSYYINNQPVRRRDVQDVFLGTGLGPRAYAIIGQGTISRIIESKPEEL
                     RLFLEEAAGVSKYKERRRETESRLSDTRENLTRVEDILRELNANLDKLEKQAEVAQQY
                     NALQAEANLKQQQQWFLRRAESAADQAKVKADAEKSVNDLESRVADLRRIESDLETIR
                     QAHYAAGDQVNQAQGQLYEASAEVGRLEAEIRFVVEGRQRVEQRLAQLKEQTAQWTAR
                     KDDALAETETLASQGAQAEEKAMLLAAQVEEQAQQLPELEEVLRQAQARSSEQQGNVG
                     QVQQQIQVLAADQRNIEEQSRQLAQRRERLAADRNALAAPDEARLTGLQAQLAVAQEA
                     AETADARLHELQEQVPQLDEDRRARQQAVNSESHKQADLSARMEALKALQEKVKTDGK
                     LRPWLAKHGLDGLQGLWSRIHIELGWENALEAALRERMNSLEVSRLDMVRAFAGDSPP
                     AKLAFYSPPQAGAPENPSPLPRLADLLRLNDAGQRALLADWLHGCYTAGSFEEALAGR
                     DKLRAGEVIYVRSGHAVTAHSVSFYAQDSEQAGLLARQQEIENLEKQLRAQALIAEEA
                     RSSLVRAEAACTDASQRLVSARREAADTRSRAHELQVETLRLAQLAEQTRARGEQIAA
                     DLAEVDTLLEELQERRVTAEGRFEELDMQLADSQERHAQLGDRVIEAERKLAECREQQ
                     RSLERQAQEAQFSQRTVEARKAELQRAIETAAEQALSIAAEEARANEELSRLTDAAAQ
                     AGLQSALALKLEREQALGAKRSEYDDLTAKLRASDERRLQLERELDPLRQRITDFQLK
                     EQAARLGFEQYSQLLADVQADLEAVARSIEEGKVRLPGMQGDIDRLHREIAALGAVNL
                     AALDELTTARERKQFLDAQSADLNEAMATLEDAIRKIDGETRELLAGTFNTVNEHFGK
                     MFPELFGGGNARLVMTGDEILDSGVQVMAQPPGKKNQTIHLLSGGEKALTAIALVFAI
                     FQLNPAPFCLLDEVDAPLDDANTERYCRLVTSMSRETQFLFISHNKIAMEMAEQLIGV
                     TMQEQGVSRIVAVDMESAVSMAEAA"
     gene            17883..>18224
                     /locus_tag="VGA59_10890"
     CDS             17883..>18224
                     /locus_tag="VGA59_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX9720227.1"
                     /translation="MSGFTVGLAVLGGLLLAILVAWNTWTTHRNTPRQPEAAAARPRP
                     LDEREDPVFDPSLVPPLPVMEKKPGLDALIDVLTPIALEGPVTGEAALAAMPPTRRAG
                     SKPFAVEGFNDI"
BASE COUNT         3078 a         6001 c         6170 g         2975 t
ORIGIN      
        1 gaggtcacct actaccggta tgtcaaactg tggctgctac agagttacgg ggtgaccgac
       61 agggccgtat gaagacggac gatggctcgc ggccggagca tgtgtagggc cgacctggtg
      121 ccacggctgc atcagcatgg ctgccggcgc aggaatggat tcctggccac cgaggttttc
      181 cacctctggc accctgaaaa tagccgggcc ggtgaagccg gcaagcgcga gcgggtcgtc
      241 cagcgcatgc aaggcagcgg caccgccccg atcgtgacgg tactgaacga atgatgaatg
      301 gagagaccaa cgagcatgaa taaccctgta ctgtggcgac gacgccaatt ggtggccctt
      361 cttggggctg cggccttgcc ggcctttgcg cagttccgcg tcgaggtgac cggggtgggg
      421 ctgacgcagc tgccgatcgc gatcgcaccg tttcgcggcg aagggcaggc gccgcagaag
      481 atcgccgcca tcgtccaggc cgacctggag cgcagcggcc agttccgcgc ggtcgatgcc
      541 gcgggagccg cgctggacga gggcagccgg cccgacctca cgccgtggcg gcagaagggc
      601 gccgattcgc tggccgccgg cagcatggca cggctggcgg acggccgttg ggacgtgcgc
      661 tttcgcctgt gggacgtggt gcgtggacag gacctgggcg gccagagcta tgccgtcacc
      721 gtgggcgacc tgcggctggc cgcccaccgc atcgccgact tcatctacga aaagctgact
      781 ggcgagaaag gcgtgttttc gacccggatc gcctacgtga ccaagaccgc caaccgctac
      841 aacctctggg tcgccgattc cgacggcgag aacgcgcagt cggcgttggc cagcgcagag
      901 cccatcatct cgccggcctg gtcggccagc ggcgcacaac tcgcctacgt ctcgttcgag
      961 tcgcgcaagc cggtggtcta cgtgcacgac gtcgccaccg gccgcaggcg cctgattgcc
     1021 aacttccgcg gttccaacag cgcgccggcc tggtcgcccg acggacgcac cctggccgtg
     1081 acgctcagcc gcgacggcgg ctcgcagctc tacaccatca acgccaatgg cggcgagccg
     1141 cggcgcctga cgcagtcggc cggcatcgat accgagccgc tctatgcgcc ggacggccgc
     1201 aacatctact tcgtgagcga ccggggcggc gcgccgcaga tctaccgcat gccgtccagc
     1261 gggggcagcc cggagcgagt cacctttacg gggagttaca acatctcgcc cgcccttagt
     1321 cccgacggcc ggtggctggc ctacatttcc cgcatcagcg gggccttcaa gctccaggtc
     1381 atggagctcg gcagtgggac cgtacactcg cttaccgaca cgaacgccga cgaaagcccc
     1441 agctttgcgc ccaatggccg cttgatcgtc tatgcgacaa ggcaaaatgg ccgggaagcc
     1501 ctgatgacga ccaccctgga tggcaagatc aaggcccgcc tgtcgggcca gagtggcgac
     1561 atccgcgagc ccgactgggg cccgtttcag aaatgacact tactggaggt atctggatga
     1621 tcaagagaat gttcctggcc gttgccgtgg ctctggctgt ggcgggatgt tcgagcgtgc
     1681 cgctcgacga tgttccggtc gaagacaaga gcgccggcgc cgtgacgggc cagcccggcg
     1741 gtccaggcag cggcacccaa agcagcgtgg cgccggtggc cgccgatccg gccatcggca
     1801 ccgtggtggg accggccaac gtggcgcgga tcatctactt cgactacgac agctacgtca
     1861 tcaagccgca gttccagtcg ctgctggacg gccatgcgcg cttcctcagg gccaacaacg
     1921 gccgccggat ttccatcgag ggccacaccg acgagcgggg cggacgtgaa tacaacctgg
     1981 cgctgggcca gcggcgctca gaggccgtcc gccaggcgct ggggctgcta ggcgtgccgg
     2041 acaggcaggt ggaggccgtg agtttcggca aggaaaaacc agtcgcgatg ggcagcgatg
     2101 aggcagcgtg gtcgcagaat cgccgggccg agatcgtcta ccgctgatga gtaccaacac
     2161 ccgccacccg ggctttcatt ggcgcgccgc cgtcgcttcg gggctggcga tgctttgcct
     2221 ggcggccagc tccgttgcgt cggccgcgct gttcgaagac gacgaggcgc gccgggccat
     2281 cctcgaattg cgccaacggg tcgatgccca gcggctggcc ggcgatcgcc aggccgagga
     2341 gctgcgccgg gcctatgagg aaaatggcca gatgcgccgc agcctgcttg agctgcagag
     2401 ccagatcgaa gccttgcggg gcgaggtcgc ccgcctgcgc ggccaggacg agcagctggt
     2461 gcgtgacctc gccgacgtcc agcggcgcca gaaggacctg gtgcaaggcg tggacgagcg
     2521 cttgcgcaag gtcgaaccgt cacaggtgtc ggtcgatggg cgcgagttca tcgccgatcc
     2581 ggccgaagcg cgcgacttcg aggccgccct ggccattttc cgccgcggcg aattcccggc
     2641 ggcacaagcc gcattcgccg aattcatcaa gcgctatccg caaagcggct tcaagcccac
     2701 cgccatgtac tggctgggca atgcccaata cgccaaccaa gactatcgcg gcgcgatcgg
     2761 gactttccgc gccctcctgg cgcagtcgcc tgaccacccg cgcgctgccg aggccgtgct
     2821 gtcgatcgcc aatagccagc tcgggctgaa ggacacgccc ggtgcacgca agaccatgga
     2881 ggagctgatc aaggcttatc cgcaatccga ggcggccgtg gcggccaagg aacggctggc
     2941 gcggttccgg tagccggggc agggcatcgc ccggcatgac ccacctcgat gaagccgccg
     3001 atctccagcg ccgtttcggc gggctggagc gcctgtatgg catgtccggc gcggcagcga
     3061 tccgggcatc gcacgtggcc gtggtcggga tcggcggcgt gggctcctgg gcggcggagg
     3121 cgctggcgcg cagcggcgtg ggcgagctga ccctggtaga cctcgaccat atcgccgaat
     3181 ccaacatcaa tcggcagatc catgccctgg ccggcacgct ggggcaggcg aaggtgcggg
     3241 ccatggccga gcgcatcgcg cagatcaatc cgcggtgcag ggtccacggc atcgaggagt
     3301 tcgtcgaacc ggacaattgg ccgcaactgc tgccggatgg cgtcgtcgcg gtgatcgacg
     3361 cctgcgatca ggtgaaagcc aaggttgcca tggccgcgtg gtcgctgcag acccgcacgc
     3421 tgttcgtggc cgccggtgcc gccggcggca agcgcctggc gcacaaggtc gacctggacg
     3481 acctggccaa caccacgcat gacccgttgc tggcgcaggt ccgcacgcgc ctgcgcaagt
     3541 tacacggcgc accgcgcgaa ggcaggaaga tcggggtagc ctgtgtgttc agccgcgagg
     3601 cggtggcccc gcccgatgcc tcctgcgcca tcgagagcga cggatcgctg aactgccatg
     3661 gctacggttc ggtcgtcagc gtcaccgcca ccttcgggca atgcgccgcg ggctgggcgc
     3721 tggaccgcct ggccacggcg aaaaccggga tcgggacgat ataatcgctg gctttgctgc
     3781 agcagcaagc aaagcagcag aggtttttga aagcgccgcg aggcggcatt tggaggcgcc
     3841 cgcaagggcg cgtgtgggac gttagctcag ttggtagagc agcggacttt taatccgttg
     3901 gtcacaagtt cgaatcttgt acgtcctacc aaatggaata aggaaggcat gctatcaaat
     3961 tggtagcagg ctttttcatt gccggcatca ttgccagaac attgccggaa ttgctggcat
     4021 tcggccggac gtcgtcagac cctgctcatg cagcacgctg gaaatcagca tcggtacgga
     4081 cgccctgggc gagcggccga gaaattcctc tcgttcaaag acctatcgcc gcaaaggctt
     4141 tgggctacga acttgccttt ggtgacgcct tcggatttga cttggctggt cagtgagccg
     4201 ggttttccct ggaccccacc atcgagtgca aggaaaccta ttgaatcttg aacataaagg
     4261 gcgcaaactg cgcctccctt cagtttcttc gctggccgta ttggcagggc actgtcgaga
     4321 ggaattggta tgcgcaactt cgtcgtgaaa ttggctgctt ggggccgggt gtgcaaggat
     4381 ctcgatgacg cgcgtctgcg gttggcgcat gcggaaactg cacaaccgcc tggccagcgg
     4441 gctcgcgcaa cggaggcgca cgttcggcgc cttgaacgtg cgcgcgatca agcgcttgga
     4501 gagcttcgga ttgccgctga agcctacatg gcaacggtgc gtcagcaaag cgcttcgacc
     4561 ccggttgaaa atcccagatc aagactcatg gatctcgatc tcgatctggg gcagggaacc
     4621 gcctgggctt gactttgcgc accggtggct ccgcaccggg cgcccagcgg cgaggcatcg
     4681 aatcgagagg gtccaccatc tgagagaggg agcaactgcg cgctggacgc ggagcatgcg
     4741 gcgcttggcg gccggaagcc gggcgacgca gccccgagtt aaaggattcc cgcgcaggcc
     4801 cgcaagtagc gcggcttacc tgtgagtcag ccgtttcttt tccggcgcgg gaagcagcag
     4861 ctgcgcctcc tgcgccacac gcacgcaatc cttgatgtgc tcttcgccca ggtagcggat
     4921 cgcggtgtag cccatgatgg ccgagaccgc ctggccggcc agcggcacgt acttggcggc
     4981 ctgcttgacg gtcaagcgcc tgccgacggt ctgggccatg cggatcacga ggtcgcgcgt
     5041 aatgaacttg ccgaccatca ccgagccgac catggccagg gccttctgca cctgctcgcg
     5101 cttgtggccg ggcaactggt cgagctgggc cggcgtcagg ccgaattccg cattgatggc
     5161 cggcaccaga cgcgacagca gggcggcgtc cacggcccaa tccaggcccg gcatgggcac
     5221 cgtgctggcc gccgcgccaa ccagggcgcg ccggttcagc agcttgcggc tgcggcgcac
     5281 ggcgtccatc agcatagcgt tgccgtcggc gagttggagt gaagtggcca tcgggttgca
     5341 gttggggtcg cgcccagtgt gcctcaagag cggctagcgc gctgtttcaa tcgcttccag
     5401 gtcgtccgac agggccagcc aacgctcttc cagcgcgcgc aactcgtcgg tgacggcctt
     5461 gaggcgcttg ccggcctggg cgatctccag cggcggcggg gccgtggcaa gcgtggactc
     5521 cagggccgcc ttgtccgcgt gcagcgcggc catgcgttgt tccgcctgtt ccagctcgcg
     5581 cttgagcggt ttggcgcgcg tggccctttg ctggcgcagc tgcgcgtcct gtttgcgctg
     5641 ttcctggggg gtgagtacgg cctgccaagc cgcgccgctg ccggcagctt gcggcgccgc
     5701 ctccggcggc cgggaatccg ccgctgccgc cgcgccttcg gcccgtgcct cctcgcgcag
     5761 acgcttggcc tcctcgagca ggtagcgctg gtaatcgtcc aggtcgccat cgaacggcgc
     5821 gatctggccg cggccgacca gccagaactc gtcgcacacg gcgcgcagca gcgcccggtc
     5881 gtggctgacc agcatgacgg tgccttcgaa ctcgttcagc gccatcgata gcgcctcacg
     5941 cgtggccagg tccaggtggt tggtcggctc atccagcagc agcaggttgg ggcgctgcca
     6001 gaccatcatc gccagcacca ggcgcgcctt ctcgccgccg ctcatggtgc ccacggcctg
     6061 cttgaccatg tcgcccgcga agttgaaagt gcccaggaag ttgcgcaggt cctgctcgcg
     6121 gctggattcg cccgaattcg gccccggttc ccgcgccagc cggaacatgt gttccagcgg
     6181 cgtgtcgaac ggccgcagca cgtcgagctc ctgctgcgcg aagtagccga tgttcaggcc
     6241 cttgccttcg gtgatggtgc cgcccagcgc cgggatgtcg cgagcgatgg tcttgaccag
     6301 tgtcgactta ccctggccgt tggcgcccag gatgccgatg cgctgcccgg ccatcacgga
     6361 gcggctgacg ccgcgcagga tgattttggg cgcggtgccc tcacccccgc cctctcccgc
     6421 agggagaggg cgcaaattcc tgtccgcctc atgttcgcgc cctctcccgc ttgcgggaga
     6481 gggctggggt gagggcagat aaccgaacac cccgtccctg atcgcgagca tcgggttcgg
     6541 caggttcgcc ggttccttga attcgaagct gaagtccgcg tcggccagca ccggcgcgat
     6601 tttctccatg cgttccagcg ccttgacgcg gctttgggcc tggcgcgcct tggtcgcctt
     6661 ggccttgaag cggtcgatga acttctgcag gtgggcgatc ttttccttct gtttcgagaa
     6721 cgccgactgc tgcagttcca gctgctgggc gcgcagcgtc tcgaatgcgc tgtagttgcc
     6781 cccgtagcgc gtgagctgct gccgttcgat gtgcaaggtg acgctcgtca cggcgtccag
     6841 gaactcgcga tcgtggctga tcacgaccag cgtgccggtg tagcgcttga gccaggcttc
     6901 cagccagacc agggcatcga ggtccaggtg attggtcggc tcgtccagca gcagcaggtc
     6961 cgaagggcac atcagcgcgc gtgccagctg cagccgcatg cgccagccgc ccgagaagct
     7021 gttcaccggc aggtacagct cgctcgcctt gaagcccagg cccaggatca gcgactgggc
     7081 ccgcggcacg gcatcgtgct cgcccgcgtc ggccaggtcc gagtgaaggt gcgcaatctc
     7141 catgccgtgg tcgtcgtgcg cgtttgcgtc gtggctcgct tcggcgcgcg ccagggcctc
     7201 gcgcaacgcg accaggcgcg tgtcgccgcc gagcacgaaa gcggtcgcgg cttcgccggt
     7261 ttcgggcatg tcctgcgcca cctgggccag gcgccactgc ggcggcatcg agaagtcgcc
     7321 gctgtcctcg tgcaggctgc cgttgaagag ggcgaacagc gtggacttgc cggagccgtt
     7381 gcgcccgacc agtccgacct tctcccccgg gttgatggtg accgagacct tgtccagcag
     7441 caccttggcg ctgcggcgaa gggtgaggtt gcgcaggacg atcatttcaa tttggcaaaa
     7501 gcctgcgcga agtcgtccag caggtcgccc tgcgcttcga tgccgaccga cacgcggatc
     7561 agcgaatcgg cgatctccat gctcgcgcgc tgctcggggc ccatctccca gaagatggtg
     7621 tgtgccaccg gcagggcgga tgtgcggttg tcacccaggt ggctggactt gatcaccagc
     7681 tccagggcgt cgagcacctg gaacggatcg acgccctcca cggtctcgaa actcagcagg
     7741 gcaccgaaat ccttgaacag gctcgcggct ctggcatgtt gcgggtggct ggccaagccc
     7801 gggtagtgca ctgcggaaac cagcggctgc gcttcgagcc attcggccag ggctctcgca
     7861 ttggcacagg tgcgttccat cctcagcgac agcgtctcgg cgcccgccgc gatacggtgg
     7921 ccatgctcgg agctgagcgt ggcaccgatg tcgcgcaggc ccttcttgcg gatctgcatc
     7981 aggccccagc ccgagggcgg gcccttgcgg taggcgggca gcaagtgggg atagcggttc
     8041 cagtcgtaca agcccgtgtc cgagatggcg ccgcccaggg cgttgccatg gccgcagatc
     8101 gacttgctca gcgaatgcac gaccagggat gcccccacgg ccttgggctg gaacaggtgc
     8161 ggtgtcgtca tcgtgctgtc caccacatac agcagcccgt gctcggtgca aagccggcct
     8221 atgccttcca ggtcggccac ctgggtgcgc ggattggcga tggtttcgac gaagaccatg
     8281 cgcgtttcgg gacgcagggc ctgccgcacg ttcgcggcat cggtcgcatc gaccaggctg
     8341 acctcgcagc cgttctgcga caaggtggcc atccagctcg cggtgttgcc gaacaggtag
     8401 cggctggcca ccacgtggtc gccctttttc agcagggcca tcatggccga gcagatagcc
     8461 gccatgccgg tggcgaagca cacggtgccc accgcgtcct ccatcaggct gatcttcacc
     8521 tccagcgcgg ccgtggtggg attgccctgg cggccgtaga catggccgct ttgccgcccc
     8581 tggaacaccg cggtgagttc ttcggccgtg ggatagccgt aggccgtggc gatgtgcagc
     8641 ggcttgagca cggcaccgtg ctccaccgag cccaggcggt cggcgtgcag gattcgggtg
     8701 gtgatgtctt ttgtcatagg gaatattgtc atcccgggct gtcatcccag gctgtcatcc
     8761 cggaccccgg atcgagtccg gggcaagcat tgatccggga tccatggatt gcgggtgctg
     8821 aaaccccgga ctcgatccgg ggccgcaatg acaagcttgg aagctgtctt gcgtgaccag
     8881 cagcacctgg tcctcgcccg cgctggtttc cagccagact acctccagcc gggggaaagc
     8941 ggcttcgaag ttgccgcgtt cgttgccgat ttcaaggacc agcacggcat gctcgctcat
     9001 gtgggccggc gcatggcgca gcagcgcgcg gatgaaatcc atgccgtcgg cgccgccggc
     9061 cagcgccatc ggcggttcgg cccggtattc ggccggcagc tcggccatgc tttgcgcgtt
     9121 gacgtaaggc ggattgcaaa ggatcaggtc gtaaggccct ggcacggacg ccagcccatc
     9181 ggactgcagc agccggatgc ggtcccgcag gccatgcttg tcgacgttga tgcgggccac
     9241 cgccagcgcg tccgccgaga cgtccgaggc gtccaccgcc acttccggat acgccattgc
     9301 ggccagcacg gccaggctgc cgttcccggt gcacaggtcc agcacgcggc tggtgcgctc
     9361 gcccagccag ggatcgatcc cgccatccac cagcagctcg gcgatgaagc tgcgcggcac
     9421 gatgctgcgc tcgtcgacat agaagggcag cccttgcagc caggcttcat gggtcaggta
     9481 ggctgcgggc ttgcgcgagc ggatgcgctc ggccaccagc tccagcacgc cgccgcgctg
     9541 ctgcgcggac acggccctgt ccgcgacgcc atccaggtcg tcgagcggca gcccgagttg
     9601 ccacagcacc agccacgcgg cctcgtcgaa ggcgttcgtg gtgccatggc cgaaggcaac
     9661 gccggcgtcg gcgagctgcc tggcgctcgc ctcgacgagt tcgatcacgg tcaccgcagt
     9721 agctcttcga gattctcgag cgtgcggcgg tagatgttct tcagcggctc gatgtccgcc
     9781 accagcacat gctcgtcgat cttgtggatg ctggcgttca cgggcccgaa ctcgaccacc
     9841 tgcgggcaga tgcgcgagat gaaacggccg tcgctggtgc cgcccgtggt ggacagcctg
     9901 gcagctatgc ccgtttcgcc gcggatggcg tcctggatcg cacccacgag ttcgcccggc
     9961 gtggtgagat acggcaatcc gccgacgacc cattccaccc catagtccag cccgtgccgt
    10021 tccagcaccc cgtgcgtgag cttttgcagg ccttccggcg tggactcggt cgaaaagcgg
    10081 aaattgaagt cgaccaccac cgtgcccggg atcacgttgc ccgcgccggt gccgccgtgg
    10141 atgttgctga tctgccagct cgtgggctgg aagaattcgt tgccccaatc ccattccagc
    10201 gtggccagtt cggccagcgc cggcagcgcc aggtggatcg ggttcttcgc gagctgggga
    10261 taggcgatat ggccttgcac ccctttgacg gtgagcctgc cgctcatgct gccgcgccgg
    10321 ccgttcttga tcatgtcgcc gaggcgttcg accgagcacg gctcgccgac gatgcagtag
    10381 tcgagccgtt cgcggcgatc ctcgagccgg tcgcagacga cgaccgttcc gtcgacggca
    10441 ggaccttcct cgtcgctggt cagcagcagc gcgatcgaca gcgccggctc gggacgagcc
    10501 gccaggaatt cccccaccgc caccacgaaa gcggcgatcg aggtcttcat gtcggcggcg
    10561 ccgcggccgt acagtttgcc gtcgcggtgc gaaggcgtga agggatcggt ggtccactgg
    10621 tccgcaggcc cggtgggcac cacgtcggtg tggccggcga acaccaccgt gcggccggca
    10681 ccggtcttgg ccagctcgcg gcttccggga cgcttggccc agagattggt aacccggaaa
    10741 gtgtcgggac cgctgaggat cgtttcgcac gcaaacccca gcggctgcag gcgctccgcg
    10801 atgatctgct ggcaatgcgc gtcttccggg gtgacggagc ggcgcgagat gagctgttcg
    10861 gtgaggtgca gagccgccat caatgcttca cgtccagcgt gatttccgtg aaggacggct
    10921 cgtcgcccag gtcctcgtcg gacgcgccgt cttccgcctg cgccccgtcc ttgcccgcga
    10981 agtcgttctg caggcgccat tgcaggttgg tcggcgaatc ggcgaaggcg agaccttcct
    11041 tcttgtccac ggtgccttcg acgatcaggc gcgcgatgtc ctgctcgaat gtctgagagc
    11101 cctcggccat ggattgctcc atcgcctcct tgacaccgga aaagtcgcct ttctcgatca
    11161 actcggccac cagcttggta ttgaggagca cttccacggc aggcgtgcgg gcgccgtgca
    11221 cggtgcgcag cagtcgctgg gacacgatcg acttgagcgc ggcggcgagg tcgccgagca
    11281 tggtcggccg cacctcgacc ggatagaacg acaggatccg gttcagggcc tggtagctgt
    11341 tgttggcgtg catggtggcc aggcacaggt ggcccgactg ggcataggca atggcagccg
    11401 acatggtttc gcggtcgcgg atttcgccga tcaggatcac gtcgggcatc tgccgcagcg
    11461 cgtttttcag cgcggtctgc atcgactcgg tgtcgctgcc cacctcgcgc tggttgacca
    11521 ccgacttctt gttcgtgaac aggtactcga tcgggtcctc gatggtcagg atgtggccgg
    11581 agaccttgtc gttgcgatgg tccatcatcg ccgccagcgt ggtgctcttg ccgcagccgg
    11641 tcgctcccac catcaacagc aggccgcgct tttccatgat caggtcggcc agcaccatgg
    11701 gcaccgacag cgagggcagc gacggaatct cggtggtgat gtagcggatc acggccgcgt
    11761 aggtgccgcg ctggcgcatc gccgacacac ggaagttgcc gatgccgtcg atggcgtggg
    11821 ccatgttcag ctcgccggtc tgttccagct cgtccatccg ctgggccggc agcacttcgg
    11881 ccagcagggc cctgggcgcg tccggcggca accgctggtt gttgatcggc aggcactggc
    11941 cattgatcct gatcagcgcc ggcgaatggg ccgacaggta cacgtccgat gctttcttct
    12001 cgctcatcag gcgcaggatt cgttccatgg tgctcattgg gtacccctca gtacttcttg
    12061 gaaaccgtca gtcgcgcagc aaatcgttca ggctggtctt ggcacgggtc tgcgcatcga
    12121 cccgcttgac gatgatggcc gcatacaggc tgtacctgcc gccgtccttg ggcaggctgc
    12181 cgctgatcac caccgagccg gccgggatgc ggccgtagct caccgtgccc gccgcgcggt
    12241 cgtaaatcgg cgtgctctgg cccaggtaca cacccatcga gatcaccgag ttctcctcga
    12301 cgatcacgcc ttccacgact tcggatctcg ccccgatgaa gcagttgtcc tcgatgatcg
    12361 tcggattggc ctgcagcggc tccagcacgc cccccaggcc cacgccgccg gaaaggtgaa
    12421 cgttcttgcc gacctgggcg caactgccca cggtggccca ggtgtcgacc atcgtgcctt
    12481 cgtcgacgta ggcgccgatg ttcacaaagc tgggcatgag gatcgcgccc ttgccgacgt
    12541 agctgccgcg gcgcgccacg gcaggcggca ccacacgcac gccggtggcc accatttcct
    12601 gcatcgacag atgcgcgaac ttggtcggga ctttgtcgaa aaagccgagg tcgccggccc
    12661 gcatgagctc gttttccctc agccggaacg acagcagcac ggccttcttg atccactggt
    12721 gcaccgtcca ctcgccgacg ccgatgcgcg tggccacgcg caagtggcca ttgttcattt
    12781 cgccgatcac atgctccacc gcgtcggcca cttccttggg cacgcaggtg ggggacaggc
    12841 tggcgcggtt ctcccacgcg ttgtcgatga tttgctggag ctgttgggtc attgatttac
    12901 ttcgtttcgg gactggacga actggacgat gcgccgcgcg gcttccacgc attccgcggt
    12961 ctcggccacc agggccatgc ggatgcggcc ggctcccggg ttgaagccgc cgctttcgcg
    13021 ggcgaggtag ctgcccggca gtacggtgac attgtattga gccagcaagt cccgcgcgaa
    13081 ttcggtgtcg cttccaggca cacccgccca gaggtaaaag cccgcgtccg gcagccgcac
    13141 gtccagcacc cgggcgagca tgggcgtgac ttcggcgaat ttcccgcggt acaggtcgcg
    13201 gttctcgacc acgtgccgct cgtcgcccca ggcggcgatg ctcgcctgct gcaccaccgg
    13261 gctcatcgcg cttccgtggt atgtgcgata cagtaggaat ttcttcatga gcgccgcatc
    13321 gcccgccacg aagccgctgc gcatgcccgg cacgttgctg cgcttggaca ggctggtgaa
    13381 ggaaaccagg ttcctgaagt ccgcccgccc cagcttcgcg gcggcctgca tgctgcccag
    13441 gggcggctcg ccggtgaaat agatctcgct gtaacactcg tccgaagcga tcacgaagcc
    13501 atggcggtcg gacagcgcga acagcttttc ccattcggcg agcggcatga cggcgcccgt
    13561 gggattgccc ggcgagcaca cgtacagcag ctgggtgcgc gaccaggtct ccagcggcac
    13621 gctgtcccag tccaccgcga aattgcgggc cgggtcgctg gcggcgtaaa agggctcggc
    13681 gcccgccagc aaggccgcgc cttcgtagat ctggtagaac gggttggggc agaccaccac
    13741 cggctttccc ccggggtcga tcacggtctg ggcgaaggcg aacaaggcct cgcgcgagcc
    13801 gttcaccggc agcatctgcg tgttggcgtc caccgccatg agcccgtaac gggtctgaag
    13861 ccagcggcga aatgcatcgc gcagatgagg cgcgccggcc gtgagcggat agctggacag
    13921 cccgctcagc gacgccgtga gcgcctgctt gatgaactcg ggcgtgggat gcttgggctc
    13981 gccgatgccc aggctgatgg ggcgataggc ggggttgggc gtcacgccca cgaaaagctg
    14041 ccgcagccgc tcgaaaggat agggctgcag gcgggacaac aggggattca tgggccgatt
    14101 atccgtgccc ccgggcccgg tttgtcagcc cggcctccct ttggatgcca tggcctcctt
    14161 gtgccggaaa gcctcgcggc ccgtggcacg gtttacgctt ggttgcaagg ctgtgcccgg
    14221 gaggcgactg gaggcgctgc cgccgcggtg agccggcggc agcacgttat catccgtgcc
    14281 actgtggccg gacccgccgg caacttcttc cgtcagcatc atcgggcagg gccgtgcgtc
    14341 tcaattccat caagttatcc gggttcaagt ccttcgccga acccaccaac ttcatgttgc
    14401 cgggccagct ggtcggtgtg gtgggcccca acggctgcgg caagtccaac atcatggacg
    14461 ccgtgcgctg ggtactgggc gagtcgcgcg ccagcgaatt gcgcggcgag tcgatgcagg
    14521 acgtgatctt caacggcacc aatacccgca aaccggcctc gcgctcctcg gtggagctgg
    14581 tcttcgacaa cgccgaccat cgggccggcg gccagtggag ccagttcacc gagatcgccg
    14641 tcaagcgcgt gctgacgcgc gacggcacct ccagttacta catcaacaac cagccggtgc
    14701 gccggcgcga cgtgcaggac gtgttcctgg gcaccggcct gggtccgcgc gcgtacgcca
    14761 tcatcgggca gggcacgatc agccgcatca tcgaatccaa gcccgaggag ctgcgactgt
    14821 tcctcgagga agcggcgggg gtgtccaaat acaaggaacg gcgccgcgag accgaaagcc
    14881 gcctgtccga cacgcgcgag aacctgaccc gggtcgagga catcctgcgc gagctcaacg
    14941 ccaatctcga caagctcgaa aagcaggccg aggtggcgca gcagtacaac gcgctgcagg
    15001 ccgaggcgaa tctcaagcag caacagcagt ggttcctcag gcgcgcggag agcgcagccg
    15061 accaggccaa ggtcaaggcc gacgccgaaa agtccgtcaa cgacctcgag tcgcgcgtag
    15121 ccgacctgcg gcgcatcgaa agcgacctgg aaaccatccg ccaggcccac tatgccgcgg
    15181 gcgaccaggt caaccaggcc caggggcagc tctatgaggc cagcgccgaa gtggggcggc
    15241 tggaggccga gatccgcttc gtggtcgaag gccgccagcg cgtcgagcag cgcctggccc
    15301 agttgaagga gcagacggcg caatggaccg cgcgcaagga cgatgcgctg gccgagacgg
    15361 aaacactggc gtcgcagggc gcgcaagccg aagaaaaggc gatgctgctg gccgcccagg
    15421 tcgaagagca ggcgcagcag ctgcccgagc tggaggaagt gctgcgccag gcgcaggcca
    15481 ggtccagcga gcagcagggc aatgtggggc aggtgcagca gcagatccag gtgctcgcgg
    15541 cggaccagcg caatatcgaa gagcagtcgc gccagttggc ccagcgccgc gagcggctgg
    15601 cggcggatcg caacgccctg gccgcgcccg atgaagcgcg cctgaccggt ctccaggcgc
    15661 agctggcggt agcgcaggaa gcggccgaaa ccgcggacgc ccggctgcac gagctgcagg
    15721 aacaggtgcc gcagctcgat gaagaccgcc gcgcgcggca gcaggccgtc aacagcgaga
    15781 gccacaagca ggccgacctg tccgcgcgca tggaagcgct caaggccttg caggaaaagg
    15841 tcaagaccga cggcaagctg cgtccctggc tggccaagca tggactcgat ggcctgcaag
    15901 gcctctggag ccgcattcac atcgagctag gctgggaaaa cgcgctggag gcggcgctgc
    15961 gcgagcgcat gaattcgctc gaagtctcgc ggctggacat ggttcgcgcc ttcgccggcg
    16021 attcgccacc ggccaagctg gcgttctaca gcccgccgca ggcaggcgcg cccgaaaacc
    16081 catcaccatt gccgcgcctg gcggacctgc tgcggctgaa cgacgccggc cagcgggcct
    16141 tgctggccga ctggctgcac ggttgctaca cggcaggcag cttcgaggag gcgctggccg
    16201 gccgggacaa gctgcgggcc ggcgaggtga tctacgtgcg cagcgggcat gcggtcaccg
    16261 cgcacagcgt cagcttctat gcccaggatt cggagcaggc cggcctgctg gcgcggcaac
    16321 aggagatcga aaacctggaa aagcagctgc gcgcgcaggc gctgatcgcc gaggaagccc
    16381 gctcgtccct ggttcgcgcc gaggccgcct gcaccgatgc gtcgcagcgg ctggtatcgg
    16441 cccggcgcga agccgccgac acgcgcagcc gggcccacga gctgcaggtc gaaaccctgc
    16501 ggctggcgca actggccgaa cagacccggg cgcgcggtga acagatcgcg gccgacctgg
    16561 ccgaggtgga cacactgctc gaggagttgc aggagcgccg cgtcaccgcg gagggccgct
    16621 tcgaagagct ggacatgcaa ctggccgaca gccaggaacg ccacgcgcag ctcggggacc
    16681 gggtgatcga ggccgaacgc aaactggccg aatgccgtga gcagcagcgc agcctggagc
    16741 gccaggccca ggaggcgcag ttctcgcagc gcacagtgga agcacgcaag gccgagttgc
    16801 aacgcgcgat cgagaccgcg gccgagcagg cgctgagcat cgcggccgag gaggcacggg
    16861 ccaacgaaga actgtcccgt ctgacggacg ccgcggccca ggcgggcctg caaagcgccc
    16921 tggccctgaa gctggagcgc gaacaggccc tgggcgccaa gcgcagcgaa tacgacgacc
    16981 tcacggcgaa gctgcgggcc agcgacgaac ggcgcctgca acttgagcgc gagctcgatc
    17041 cgctgcgcca gcgcatcacc gacttccagc tcaaggagca ggccgcgcgc ctgggtttcg
    17101 agcagtacag ccagttgctg gccgacgtgc aggccgatct cgaagccgtt gcccggtcca
    17161 tcgaggaggg caaggtgcgg ctgcccggca tgcaaggcga catcgaccgc ctgcaccggg
    17221 aaatcgccgc actgggcgcg gtgaacctgg cggcgctcga cgagctgacc actgcgcgcg
    17281 agcgcaagca gtttttggat gcgcagtcgg ccgacctgaa cgaagcgatg gctaccctgg
    17341 aagatgccat ccgcaagatc gacggcgaaa cccgcgagct gctggccggc accttcaaca
    17401 ccgtcaacga gcatttcggc aagatgttcc ccgagctgtt cgggggcggc aatgccaggc
    17461 tggtgatgac cggcgacgaa atcctcgatt cgggcgtgca ggtcatggcc cagccgcccg
    17521 gcaagaagaa ccagaccatc cacctgcttt ccggcggcga aaaggcgctc accgccatcg
    17581 cgctggtgtt cgcgatcttc cagctcaacc ccgcgccgtt ctgcctgctc gacgaggtcg
    17641 acgccccgct ggacgacgcc aacaccgagc gctattgccg gctggtcacc agcatgagcc
    17701 gcgaaacgca gttcctgttc atcagccaca acaagatcgc gatggaaatg gccgagcagc
    17761 tgatcggcgt gaccatgcag gagcaaggcg tttcgcgcat cgtggcggtg gacatggaat
    17821 cggccgtttc gatggccgag gcggcatgag cgcgaagggc gaggcatgac ggcgagggca
    17881 cgatgagcgg ttttaccgtc gggctggcag tccttggcgg cttgctgctg gcgatactgg
    17941 tggcctggaa cacctggacc acccacagga acacgccgcg ccagcccgaa gccgcggcag
    18001 cccggccgcg accgctcgac gagcgtgaag atccggtgtt cgacccgagc ctggtgccgc
    18061 cgttgccggt gatggagaaa aagcccggcc tggacgcgct gatcgacgtg ctgacgccca
    18121 ttgcgctgga agggcccgtc acgggggagg ccgcgctggc ggccatgccc ccgacccgcc
    18181 gcgccggcag caagccgttc gccgtggaag gcttcaacga catc
//
LOCUS       DASWIY010000296        21540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2785755, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000296 DASWIY010000000
VERSION     DASWIY010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 21540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21540
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2785755"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..187)
                     /locus_tag="VGA59_10895"
     CDS             complement(44..187)
                     /locus_tag="VGA59_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720228.1"
                     /translation="MNSAAFQKARADVGLPMVRVHVAQKVAQTPFGGANVGWGITPPN
                     GPR"
     gene            complement(360..2807)
                     /locus_tag="VGA59_10900"
     CDS             complement(360..2807)
                     /locus_tag="VGA59_10900"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9720229.1"
                     /translation="MTRSSKTQASSPRRAPAEPRLSRTRQPDTMSVEDWQASLRRQFG
                     REQSFVLERLGAEPEFVVSNPQSKGRYRVALRGMEPGDNFCSCPDFATNDLGTCKHIE
                     FTLARLLSRRDGKAMLASGFAPPYSEIYLRYRGERSLCFRAGTECPPALATKGAAMFG
                     GGAAHALELEGFLRAAHKARHEVRCYDDARAFVAERRDAERRRRIFAETYPRGAKDAH
                     LRKLLKAPLYPYQAEGALFAATAGRALVADDMGLGKTIQAIAAAELLSRHCGAERVLV
                     ICPTSLKHQWQREIDRFSGRGAFVIRGLRSARAQQWADASERAGWAKITNYETLARDL
                     DLINAWAPDVVIVDEAQRIKNWNTVAARALKRIASPYAIVLTGTPLQNRLEELVSIVQ
                     FVDQHRLGPTWQLLHAHQLRDESGRVVGYRDLDRIGATLAPVMLRRRKAEVLSQLPER
                     VDNTLFVPMTGPQLAHHNENGNIVRRIVQRWRKTGFLSDTDQRLLTCALQNMRMSCNS
                     TYLLDHETDHGTKADEVVALLEDLFEQPDAKAVVFSQWVRTHELIIRRLQARGWSHVL
                     FHGGVPGDQRPALIDRFHDDPQCRVFLSTDAGGVGLNLQHAAAVIVNMDLPWNPAVLE
                     QRIGRVHRLGQRKSVHVVNFVAQGSIEEGMLSVLAFKKSLFAGVLDGGESEIFLQGTR
                     LSKFMDTVDEVTGAIGPAEVAEASAPDVAAAQTAGHVPGEVLPAATAEPRPTASQEPA
                     AADPWAALVDAGLELVELLTRSRSGDATEPGMAIERDPRTGRDVLKIPLPQPQTVQRL
                     ADALGGLLSGLRREGTP"
     gene            complement(2931..3605)
                     /locus_tag="VGA59_10905"
     CDS             complement(2931..3605)
                     /locus_tag="VGA59_10905"
                     /inference="COORDINATES: protein motif:HMM:NF021567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HEX9720230.1"
                     /translation="MPESQDLKVFIAAGEPTCGECGSVLDRHAWIMLAGERGALCLAC
                     ADLDHLVFLPAGDVALTRRARKHSALAAVVLKWSRARKRYERQGLLVEEDALARAEAE
                     CLGDADARARAREREAARRAGTDREYLDRFAQRVRELFPHCPPGTELAIARHACLKFS
                     GRVGRSAAAKALDEQAVQLAVAAHVRHAQTGYDVLLATGHERHEARRQVQARVTQVLA
                     TWRGTP"
     gene            complement(3769..4596)
                     /locus_tag="VGA59_10910"
     CDS             complement(3769..4596)
                     /locus_tag="VGA59_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HEX9720231.1"
                     /translation="MDRSSSHVDPLVIAAEALAALDQGRQIAPFSARYQGFDLDAAYR
                     AAAHVRALRCARGETPIGRKIGFTNSTIWAEYDVWAPIWGDCYDRTVRNAPTDGAAFG
                     LRNLAEPRIEPEIVFGIAMAPAAGMDAAALLDCIGWVAHGFEIVQSIFPGWKFAAADT
                     VAAFGLHGALLIGPRHEVAPRRQQWLATLASFQIDLFCDGVIADRGRAANVLGGPLLA
                     LRHLVEFLARDPAQPPLAAGEIVTTGTLTRAFPIVPGQTWSTALYGVALEGLAVRFD"
     gene            complement(4861..6393)
                     /locus_tag="VGA59_10915"
     CDS             complement(4861..6393)
                     /locus_tag="VGA59_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9720232.1"
                     /translation="MNYFLSALLRPGVQWMSSKSLRTRLAVVGYMLILPLVGIVLAQW
                     RDTSRDWVLAGGILAVVAGAYALAVLYRSVMDAVDALQTVVHAGSRGDLTVLATASGA
                     DELARIGQNLERMNEGLSGVVGQVRSQTVLVAMAGEMLASNSRELSQRTDAQAASLEQ
                     SSASIQGLSGSVRNNAGSAQTIDQLARRVRLEAEAGVQAMGVAEQAMRQLDEQSRRMD
                     EIIGVIDGIAFQTNILALNAAVEAAHAGEAGRGFGVVAAEVRTLAKGSSESAREIRNL
                     VRQSAEQVDVAVARIAAASSSLSTIAQGVGELAAGVSGIARANGEQNMALQEITQAVK
                     VLEDITQRNATMAEQVSNAAGQLKERAAGLGAAVLGVRLRRGSADEAYALVRKAQAFV
                     ENRGMDAAVAEFHRPPGQTAFRDRDLYIFVFDRRGHYTVFGCAPERIGTTTHQVPGLD
                     GDLVLQRGFAAADAGGGWIEYEIVHPQTREVEEKVSYVELLSADTLIGCGVFKPNSGF
                     AH"
     gene            6806..9055
                     /locus_tag="VGA59_10920"
     CDS             6806..9055
                     /locus_tag="VGA59_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HEX9720233.1"
                     /translation="MNLPHIRRRPRLPGVTVFLLAACVWLPASAAPAQPAAAALPPMA
                     AASAPSASEVFRRMSADQWIARVVTFADLGFKGPLVLGYPETQREITLPVPPGVPLAN
                     ATLQVDASFVRADGGRTTLILSLDGFPVSARPVTAERGDGSLTLAVDGAPRASGLVRF
                     NIDWRTAIGRENTCTDSRTPGNLLRIEPSTRFTYRYDGSAVQDLSTAWAALPSSPVIL
                     IAGNKLSADAYDSAWRVGVALDRAGKRPRIRSLPAVGDVVDLRGVVVPTALRRMPAFA
                     ALADGGKRRIKDLAEIGALMALGQGGPLQPDIVIGDRATGSVLAEALDELRAQLPAEV
                     IDAFSEWRGHALDGWGRQLAAGQVRLTNVFGRPAIVIAPDAGGHAAALFTQSWQQVAM
                     GPLLTVHAADEQKADLATVSLKYLGAKPATLDVLARGDWNAGFDIGAVAMDGRGPGTL
                     VVDVAAAPGAARTPPVVSVFLNEVLLAAKEMEANGRRERIVAPIPRHVLSTRNDIRVS
                     FVRQQASDRCRESPEPYPVSVLSSSHIVLDKIEPMGDFSGLISRFASGVNLLVPIAYL
                     YDAQNTLPRVISLASATGVAPARARFIPVADVGPPKIKGPFLAIDVALKDADQSEVKV
                     ERGRLFLSDGSERPLLDVSGLNRAGLLEVARVGRDAGAIYRTLGRDAPTMDIPMRLSG
                     GNIAVIGMDGLRAEVNTVDPSGQGMAREARPSLTDRGFWWLLPLLVVAFVAALLAYAY
                     RVHRRQANG"
     gene            complement(9068..9481)
                     /locus_tag="VGA59_10925"
     CDS             complement(9068..9481)
                     /locus_tag="VGA59_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9720234.1"
                     /translation="MSFPVELQAYVVEDNPTIRENLVGTLEELTCVKVAGTSATEDEA
                     LAWIDKNMDRWDLLIVDLFLKQGSGMHLAQLMSRRRPNQKVVVFSNYINASVRKRCAQ
                     LGVDAVFDKSTEIDALVDYCSHLCFRVAQAQAAAA"
     gene            9622..12081
                     /locus_tag="VGA59_10930"
     CDS             9622..12081
                     /locus_tag="VGA59_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9720235.1"
                     /translation="MDRPSIAIEPDRLQRTESLLKLLERAAFIGVWTLDLESDRLEWS
                     DQLAAIHDAAPGFTPTRDDAFALYAPEWRELVRALVQGCATAGEPFDEEMQILTLRGR
                     RAWVRTLGHAVRDEAGNIVRIEGAVQEIAPHGHRAGTLLRHTVSMGGAMGGVEAFATI
                     DREGRLTYLNEQAELLLGKGAPELLGRKIWNSFQKTVRLRLEEQFRDAFESEKIVEVE
                     ELDANLSRRIEARGYPFGAGLAVHLRDVTARHKSQEQLRLLESSIARLNDIVIITEAG
                     PFNAPGPRIVFVNEAFERRTGYSPQEVMGRTPRLLQGPATQREELDRIRSAMEQWQPA
                     RVDLINYKKNGEAFWVDLEVSPVWDHERKLTHWVAVGRDITERKTAEEKIQYLAFYDP
                     LTRLPNRQLLLDRLQRALSDQDRPREGALMFIDLDNFKVLNDTMGHHKGDLLLQQVAE
                     RLRSCVARGDTVARLGGDEFVILLENTGHKPLEPAAGARIVSERILARLGETYLLAGH
                     LHHSTCSIGVTLFGRAPWTVSELLKQADLAMYQAKGAGRNAVRFFDPEMQAVVTANAA
                     LATDLREAWRESQFLIDYQPQVGADGRMTGVEALLRWRHPQRDMVPPAHFISTAEETS
                     LIIPIGHWVLEQACAQLAQWATRPERRHLSIAVNVSVRQFRHPDFVDEVMTAIENSGI
                     APHKLKLELTESLLADGIEVTVAKMGRLKEMGVTLSLDDFGMGYSSLSYLKRLPLDQL
                     KIDREFVKDILTDANDAAIARTIVGLAQSLGLGVIAEGVETQAQRAFLAQQGCYAYQG
                     FLFCRPLPIEQLEVFMDEVEP"
     gene            complement(12104..12919)
                     /locus_tag="VGA59_10935"
     CDS             complement(12104..12919)
                     /locus_tag="VGA59_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720236.1"
                     /translation="MKRDTRGAEDDLEMQGRQGRWGTGEVVPMPLVADAHHGEGSASV
                     ARRPRVAGAEAARYGVLRRLAPALKHDMVVNLQAISMMAEVMSARLERGSSAPADFQS
                     SISKLNRLARDAVMNCLKVAAWIEPGEDEGVRLARGIEDCLALLSTNFNFRGFSIVSE
                     VPDTDFEVWRVTLRNLLMASLLALTDAAPGPSEVRVKAEVQGGFAEISVRVTPRQDEF
                     EALPFGPSYRLLDWSDVQALAVAESVELVRGPDQIVMRMPRAVATAPLRIAPI"
     gene            13163..13744
                     /gene="greB"
                     /locus_tag="VGA59_10940"
     CDS             13163..13744
                     /gene="greB"
                     /locus_tag="VGA59_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEX9720237.1"
                     /translation="MSKAFTREPDVDDEEEASLPELGSGGKNYITPQGYARLRAELLQ
                     LMDEERPKVVEAVHWAALNGDRSENGDYIYGKKRLREIDRRIHFLTHRLEIAEVTDPA
                     VHHGGDQVFFGASVTYAEETGQERTVTILGIDEADSAHGQVSWISPIARALLKARVGD
                     VVKLVTPAGTHEIEVLRVNYPAPAAGPDRDTRG"
     gene            complement(13757..15982)
                     /locus_tag="VGA59_10945"
     CDS             complement(13757..15982)
                     /locus_tag="VGA59_10945"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX9720238.1"
                     /translation="MAEAAASPAAANAEAASFAALTARLDYLDPAEAEQVRKAYRFAD
                     EAHLGQIRASGEPYITHPIAVAAQCAEWKLDAQALMAALLHDAIEDCGVTKPQLIERF
                     GAPVAELVDGLTKLDRLQFDTREESQAESFRKMLLAMARDVRVILIKLADRSHNMRTL
                     VDVPREKWARISSETLDIYAPIAHRLGLNQTYRELQELAFRHLRPWRHAILTKAVTKA
                     RNRRRDLIQKVQREVESAFETAGMNVRIAGREKTLYSVYRKMDEKHLSFAQVTDIYGF
                     RVIVPTVTDCYTALGVLHQMYKPVPGKFKDHIAIPKVNGYQSLHTTLVGPSGVNVEFQ
                     MRTEAMHVVAESGVAAHWLYKASGPEGEGGDRLGTKWLQSLLDIQDETRDAAEFWDHV
                     KIDLFPDAVYVFTPKSQIMALPRGATVVDFAYAIHSDIGDRTVAAKINGLQVPLRTEL
                     KNGDVVEVVTAPVSTPNPAWLGFVRTGRARSKIRHHLKSMAQAESLELGQKLLTQALR
                     AEGLEKLPADDEEHRPIWEKLLRFTGNRSRAELLTDIGLGKRIASIVAKRLVTMLAER
                     GEKPDALLITRERYTAHENVSQGEIILDGSENASVQFATCCRPVPGDAIVGYLGRGEG
                     LVVHTEDCAVAKRLQHKDSERFISVEWADEPVRPFETGLLVSVENGKGVLARVAAALA
                     GAEADITHVDMGDEKAQDATDLRFIVAVRDRAHLETAMRALKRTPSVIRVQRARPGST
                     F"
     gene            complement(16303..16506)
                     /gene="rpoZ"
                     /locus_tag="VGA59_10950"
     CDS             complement(16303..16506)
                     /gene="rpoZ"
                     /locus_tag="VGA59_10950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX9720239.1"
                     /translation="MARITVEDCLQHIPNRFQLVLAATYRARMLSQGHAPKIESKNKP
                     GVTALREIAQGKIGLEMLKKVPG"
     gene            complement(16547..17167)
                     /gene="gmk"
                     /locus_tag="VGA59_10955"
     CDS             complement(16547..17167)
                     /gene="gmk"
                     /locus_tag="VGA59_10955"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX9720240.1"
                     /translation="MDYPGNLFVVAAPSGAGKSSLVNALMELDARVQPSVSHTTRPPR
                     GQEKHGREYFFVSPPEFDAMVLADAFVEWAFVHGHRYGTSKKAIEERIAQGADVILEI
                     DFQGALQIKRIFGNAVLIFILPPSWEELRSRLERRGEDSAEVIDMRLKNAAEEIAQVR
                     EFDFVIINELFDRALFDLKAVVHAQRLKFSAQRRARADTFKALNIT"
     gene            17485..19935
                     /locus_tag="VGA59_10960"
     CDS             17485..19935
                     /locus_tag="VGA59_10960"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX9720241.1"
                     /translation="MTDDEFPYSRSLAPLESLLAGVKRPGDFCVHGALALPMPMVQVD
                     GVGLLSFPLPPAQIAALIAAAERAPYGRGEQTVVDTSVRRVWQIDAARVRISGKSWTQ
                     SLGRILAETGRGLGCEDTAIQAELYKLLVYDPGGFFLAHRDSEKAPGMFGTLLLQLPS
                     PHSGGELRIRHGSREARIDLSATDCSEFGFAAFYADCEHEVRPVESGHRVCLVFNLLR
                     GGASDTPPASAPSHDAEVAQAAWLIGAAFAAEGAPRKLVWLLAHQYSQAELRLAALKG
                     ADAAVAGVLCRAAEAAACRAHLGVVHIEESGPAEVHYAPSRGRRYHDFGDFDDIDATG
                     ADYEVVEISDTTRYIDHWVAPGDQKPALGRLPLGPGELLPAGALDDEPPDEDRLLEST
                     GNEGVSFERAYRRAALVLWPRARQVEVLLQAGVAAALPMLEEALAADAADAGAAALDV
                     AERIVAHWSRQSPPRYAAAREGPLRARMLRCLARLGTTAAGERFLREVLVADYDGSEN
                     AALAEAAPAMLAAQPLQELLAALVESAFLRCPRALCELLFLLDAGNQAQPSLAGDLAL
                     RRFAGALVDALPGLAARPSRSDLGWVLQHDETAADAESCHRLLGVLERLASGEHHAAA
                     VTTLIAQPQVFDPGRVLVPALQALCEITQGLKSRAGAERGGIDRDAQRLRLWRHCCAL
                     LLARSEFAPPAPRDWSQPAVLACRCEDCRALQAFALDPRLREQRFRVRQDRREHLQRQ
                     IAQHGLDMHHVTDRSGSPQTLVCRKTRESYRRQCRQHAEDIAAMRALQALAVAAPNAQ
                     ESARLAAAVARQPQAVEG"
     gene            19939..>21540
                     /locus_tag="VGA59_10965"
     CDS             19939..>21540
                     /locus_tag="VGA59_10965"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX9720242.1"
                     /translation="MAFHAAEAQPAPASATLHWQPNHVDLSSTARAKVALFRSLFRGR
                     DDLYARRFESATTGKSGYAPACASEWVPGICRKPRIKCADCAHRAFLPLTDQVVYWHL
                     CGHAPGEPPHRPFVAGVYPLREDETCFFLAADFDKAGWQDDAAAFVQACRRLHLPVAL
                     ERSRSGEGGHVWLFFETALPAAMARRLGSHLLTEAMEARPDIGLDSYDRLFPSQDTMP
                     RGGFGNLIALPFQKEPRELGNSVFLNDGFMPWADQWAFLANVGKIGRAQVEGIVREAE
                     QRGRILGVRLPMQEEGEDEPWTLPASRRRKEPPLAGELPPTLELVLADQIYIARQGLP
                     PGLMNRLMRLAAFQNPDFYRAQAMRLSTYGKPRVITCAEDHPLHLALPRGCLDDVRRT
                     LKDLGVRVTLRDERCSGQPLDLRFHGELRPEQTSAAQALLAHQTGVLAATTAFGKTVV
                     AAWLIAQRGVNTLVLVHRQQLLDQWIARLSAFLDLPANAIGRIGGGRRRPNGRIDVAL
                     IQSLVNKGVVADEVANYYGQLIVDEC"
BASE COUNT         3360 a         7217 c         7343 g         3620 t
ORIGIN      
        1 tccatcgtca gctccgttct gctcaatctg aagccggatc ggatcacctc ggaccgttcg
       61 gcggtgtaat gccccatccc acgttcgcgc cgccgaacgg cgtctgcgcg actttttgcg
      121 cgacgtgcac gcgcaccatg ggcaggccca cgtcggcgcg ggccttctga aatgcagcgg
      181 agttcatggt gtcgacgcgt gatcatgccg atgctcgagc agggtgcgtg cggtcgtgcg
      241 ggaaactaac ggctaactgc ggcttggctg gcggcacgac gcggcgtgag cctcgccgtg
      301 gtcggcacag gacgatcacc ccaggcgggc ggtgcgcaag ccggcaaatg ggaccggcat
      361 catggcgtgc cttcgcgccg caagcccgac aggagcccgc cgagcgcatc ggccaagcgc
      421 tgcacggttt gcggctgcgg caaaggaatc ttcagcacgt cgcgcccggt acgcggatcg
      481 cgttcgatcg ccatgccggg ctcagttgcg tcgccgctgc ggctgcgggt gagcagttcc
      541 accagttcga gccctgcatc gacaagtgca gcccaaggat cggcggcagc cggctcttga
      601 ctcgcagtgg gccggggctc tgcggtggcc gctggaagta cctcgcccgg cacgtgcccc
      661 gcggtttgcg cggccgccac gtcaggcgcc gacgcttcgg caacctctgc cgggccgatc
      721 gcgccggtca cttcgtccac cgtgtccatg aacttcgaca agcgcgtgcc ctgcaggaag
      781 atctcactct cgccgccgtc gagcacaccg gcgaacagcg acttcttgaa tgccagcacc
      841 gacagcatgc cctcctcgat gctgccctgc gcgacgaagt tgacgacgtg cacactcttg
      901 cgttgcccga gccggtgcac gcggccgatg cgctgctcca ggactgctgg attccatggc
      961 aggtccatat tcacgatgac cgccgcggcg tgctgcagat tgaggccgac gcccccggcg
     1021 tcggtggaca ggaacacgcg gcactgcggg tcgtcgtgaa agcggtcgat cagcgccggc
     1081 cgctggtcgc ccggcacgcc gccgtggaac agcacatggc tccagccccg ggcttgcagg
     1141 cgccggatga tcagctcgtg cgttcgcacc cactggctga agaccaccgc cttcgcatcg
     1201 ggctgctcga acagatcctc cagcagcgcc acgacttcgt cggccttggt gccgtggtcg
     1261 gtctcgtggt cgagcaggta ggtgctgttg cacgacatgc gcatgttctg cagcgcgcag
     1321 gtcagaagcc gctggtcggt atcggacagg aagccggtct tgcgccagcg ctgcacgatg
     1381 cggcgcacga tgttgccgtt ctcgttgtgg tgcgcaagct gcgggccggt catgggcacg
     1441 aacaaggtgt tgtcgacgcg ctcgggcagc tgggacagca cctcggcctt gcgccggcgc
     1501 agcatgaccg gcgccagtgt cgctccgatg cgatccaggt cgcgatagcc gacgacgcgg
     1561 ccggactcgt cgcgcagctg gtgcgcgtgc agcagctgcc aggtgggtcc aagccggtgc
     1621 tggtcgacaa attgcacgat cgacaccagt tcctccagcc ggttttgcag cggcgtgccg
     1681 gtgagcacga tcgcgtaggg gctcgcgatg cgcttcagcg cccgtgccgc aacggtgttc
     1741 cagttcttga tgcgctgcgc ctcgtcgacg atcacgacgt cgggcgccca ggcattgatg
     1801 aggtcgaggt cgcgggccag tgtctcgtag ttggtgatct tggcccagcc agcgcgctcg
     1861 gaagcatcgg cccattgctg cgcacgcgcg ctgcgcagcc cccggatgac gaaggccccg
     1921 cgcccgctga agcggtcgat ctcgcgttgc cactggtgct tcagtgacgt ggggcagatc
     1981 acaagcacgc gctcggcgcc gcaatggcgg ctcagcagtt cggcggcggc gatcgcctgg
     2041 atcgtcttgc ccaggcccat gtcgtcggct accagcgccc ggcccgccgt ggcggcgaac
     2101 agcgcgcctt cggcctggta cgggtacagc ggcgccttca gcagcttgcg caagtgggca
     2161 tccttcgcgc ccctggggta cgtctcagcg aagatgcggc gccggcgctc ggcgtcccgc
     2221 cgctcggcaa cgaacgcacg ggcatcgtcg tagcatcgga cttcgtggcg ggccttgtgc
     2281 gcggcccgca ggaacccttc gagctccagg gcatgagcgg cgccgccgcc gaacatcgcc
     2341 gcgccctttg tcgccagcgc cggcggacac tcggtgccag cccgaaaaca caagctgcgc
     2401 tcaccgcgat agcgcaggta gatctcgcta tacggcggcg cgaagccgct tgccagcatg
     2461 gctttgccgt cgcgccgtga cagcagcctc gccaacgtga actcgatgtg cttgcaggtg
     2521 ccgagatcgt tggtcgcgaa gtcggggcac gagcagaagt tgtcgcctgg ctccatgccg
     2581 cgcagcgcga cgcggtagcg gcccttgctc tgcggattgc tcaccacgaa ttccggttcg
     2641 gcgcccaggc gctcgagcac gaacgactgt tcgcggccga actgccggcg caggctggcc
     2701 tgccagtcct ccaccgacat ggtgtcgggc tggcgggtgc gcgacaggcg gggttcggcc
     2761 ggggcgcggc gcggtgatga ggcttgggtc ttggacgagc gggtcatggc tgatggcgaa
     2821 gcaagcacgg attgtcgtct accggcgctg ccgcttccgc gtcggccggc gacgcggccc
     2881 cggtggcttg cccagccgga cgaagtggcg cggttcgaga cctggccgct tcatggggtg
     2941 ccgcgccacg tcgccagcac ctgagtcacc cgagcctgca cctggcgccg cgcctcgtga
     3001 cgctcgtgcc ccgtcgcgag cagcacgtcg tagccggtct gcgcatggcg cacatgggcg
     3061 gcaacggcaa gctgcacggc ctgctcgtcg agcgccttcg cggcggcgct gcggccgaca
     3121 cgaccgctga acttcaggca ggcgtggcgc gcgatcgcca gctcggtgcc cggcgggcag
     3181 tgcggaaaga gctcgcgtac acgctgcgcg aatcgatcga ggtactcccg gtcggtcccg
     3241 gcgcggcgcg ccgcttcacg ttcgcgcgct cgtgcacgcg cgtcggcgtc gcccaggcat
     3301 tccgcctcgg cacgggcgag cgcgtcttcc tcgaccagca ggccctgacg ttcgtagcgc
     3361 ttgcgcgcgc ggctccactt cagcacgacg gcggcgagcg ccgagtgctt gcgcgcgcgg
     3421 cgcgtcagcg cgacatcgcc ggcgggcagg aacacgagat ggtcgaggtc ggcgcaggcg
     3481 aggcagagcg caccgcgctc gccggccagc atgatccatg catggcggtc gagcacgctg
     3541 ccgcactcgc cgcaggtcgg ttcacctgcg gcaatgaaaa ccttcagatc ctgggattca
     3601 ggcataggga gtcactcatt tcaccggtac gaccgtcatg atgcccagat cccgcgccac
     3661 ggcttcgcgc cgccttcgcg cggccggcac cccctcgcgt caatgacacg caaatttagt
     3721 gtcgcgacgg gccatcgagc gagctttcac cggagtgccc ccggggcgtc agtcgaaccg
     3781 aacggccagc ccttcgagtg ccacgccgta caaggcggtc gaccaggtct gtcctggcac
     3841 gatggggaac gcgcgcgtga gcgtccctgt cgtgacaatt tcgcccgcgg ccagcggcgg
     3901 ttgcgccggg tcgcgggcga gaaattccac caggtgccgc aatgccagga gcggtccccc
     3961 gagcacgttc gccgcgcgac cccggtcggc aatgacaccg tcgcaaaaaa ggtcgatctg
     4021 gaacgacgcc agcgtcgcca gccactgctg gcgccgcggc gcgacctcat ggcgcgggcc
     4081 gatcaggagc gcaccgtgca agccgaaggc cgcgacggtg tcggccgcgg cgaacttcca
     4141 gccggggaag atggactgca cgatctcgaa gccgtgtgcc acccagccga tgcaatctag
     4201 caatgctgcc gcatccatgc cggcggccgg cgccatcgct attccgaaaa cgatctcggg
     4261 ctcgatccgg ggttccgcca gatttcgcag gccgaatgcg gcgccatccg tgggggcatt
     4321 ccgtaccgtt cggtcataac aatcgcccca gatcggagcc cagacgtcgt actcggccca
     4381 gatcgtgctg ttggtgaacc cgatctttct gccgatcggc gtttcgccgc gcgcgcagcg
     4441 cagggcgcgg acatgagcgg cagcgcgata agcggcatcc aggtcgaacc cctgatagcg
     4501 cgccgaaaac ggcgcgatct gccggccctg gtcgagggcg gcaagggcct cggcggcgat
     4561 gaccagcggg tcgacgtggg aagaggaacg gtccatggag tcgcccgccc gcagggatgg
     4621 gaatcggccc gaacgcgggg tgcatccgtt gttcaaaagg tgattctgtc gcagcggctt
     4681 tatttgtgcc actgctcgcc gtcgcggctg taagtaccgc tcagccgttg gaacagccac
     4741 tcgttatttg gcttgcgtga gtcatgacct tcgccgactt ggcctgcggc ccgctacggg
     4801 ccggcgagag aacagatgtt tacgggacag actgaggcag cgagctcatc ctttgcgcag
     4861 tcaatgcgca aacccgctgt tcggcttgaa cacgccgcag ccgatcaggg tgtccgcgga
     4921 caacagttcc acgtaggaga ctttctcctc cacttcccgt gtctgcgggt gcacgatctc
     4981 gtactcaatc cacccgccac cggcgtccgc ggccgcgaat ccccgctgca agaccaggtc
     5041 gccgtccagc cccgggactt gatgcgtagt ggtgccgatc cgctcaggcg cgcagccgaa
     5101 caccgtgtag tgccctcggc gatcgaagac gaaaatgtac aggtcgcggt cgcggaaggc
     5161 ggtctggccc ggtggccggt gaaactcggc cacggccgcg tccatgccgc ggttctcgac
     5221 gaaggcctgg gctttcctga ccagcgcata cgcttcgtca gcgctgccgc gccgcaggcg
     5281 cactcccagc acggcggcgc ccaacccggc ggcgcgctct ttgagctggc ccgctgcgtt
     5341 ggagacttgc tccgccatcg tcgcattgcg ctgcgtgatg tcctccagga ccttgaccgc
     5401 ctgggtaatt tcctgcagcg ccatattttg ttcgccattg gctcgggcga tgccgctcac
     5461 acccgctgcc agctcgccca cgccctgcgc tatcgtggac aggctgctgc ttgcggcggc
     5521 gatgcgcgcg acagcgacgt ccacctgctc ggcgctctgc cggaccaggt ttcgaatctc
     5581 gcgcgctgac tcgctgctgc ccttggccaa ggtgcgcacc tcggcggcca caacaccgaa
     5641 gccacggccc gcctcacctg catgcgctgc ttccacggca gcattgaggg ccaggatgtt
     5701 ggtctgaaag gcaatgccgt cgataacgcc gatgatttca tccattcggc gcgattgctc
     5761 gtccagctgg cgcatcgcct gctcggccac acccatggcc tgcaccccgg cttcggcttc
     5821 cagccgaacg cgacgggcga gctggtcgat ggtttgggcg ctccccgcgt tgttgcgcac
     5881 ggaaccgctc agcccctgaa tgctggccga cgattgctcc aggctcgcag cctgcgcatc
     5941 ggtccgctgg gacagttcac ggctgttgct cgccagcatc tcacccgcca ttgcaaccag
     6001 caccgtctgg ctgcgcactt gccccactac gcccgacaag ccctcgttca tgcgctcgag
     6061 gttctgcccg atgcgcgcca gctcgtcggc gcccgatgcg gttgccagca cggtcaggtc
     6121 gccgcgcgaa ccggcgtgca ccactgtctg caaggcgtcc acggcatcca tcacactgcg
     6181 gtagagcacg gccaaagcgt acgcgccggc cacgacggca agaataccgc cggccagcac
     6241 ccagtcgcga gacgtgtcgc gccactgcgc caacacaatg ccgaccaggg gcagaatgag
     6301 catgtatccg accactgcca gtctggtacg caggctcttg ctgctcatcc attggacacc
     6361 tggccgaagg agtgcgctga gaaaatagtt catctattga gctccgcatt cggtcacgca
     6421 ggggtgccaa cccactgaat gccgctggaa ctcatcaatt caaccataag aacaacgaaa
     6481 ccggggggtt ttcgcaatcg atggcgaggc ggcgttgcaa cggcacgccg cctgcgcgga
     6541 atttcagggt aggcagcctc ctacatcctg acaggagctt ggctgtcgag ccgccttcgt
     6601 tacaaattct agattcgagg ctggatttga tacacccaag ctcagcccga gcggcgatgc
     6661 ttgagccgtg tgcctcagca gtagcgtaag cggtttcgca ggggggtccg tgcaatggca
     6721 ggcggatgct acccaggaag tcgctgcgcc tcgtgtggcg gaatcggagc gaaccgtgca
     6781 gaaaccgttt ggtcgcgagc gtgtcatgaa cttgccgcat atccgccgcc gcccgcggct
     6841 gcccggcgtg acggttttcc tcctggccgc ctgcgtctgg ctcccggcgt cggcagcgcc
     6901 ggcccagccc gccgcggccg cattgcctcc gatggccgcc gcctccgcgc cgtcggcgtc
     6961 cgaggtgttc cggcgcatga gcgcggacca gtggattgcg cgcgtggtga ccttcgccga
     7021 cctgggcttc aaggggccgc tggtgctggg ctacccggaa acccagcgcg agatcacgct
     7081 gccggtgccg ccgggcgtgc cgctggccaa tgccaccctg caggtggacg cgagcttcgt
     7141 gcgggccgac ggcggccgca ccacactgat cctctcgctc gatggcttcc cggtttcggc
     7201 acggccggtg acggcggagc gcggcgacgg cagcctcacg ctggccgtcg atggcgcgcc
     7261 gcgggccagc ggcctggtgc ggttcaacat cgactggcga accgcgatcg gccgcgagaa
     7321 cacttgcacc gattcgcgca cgcccggcaa cctgcttcgc atcgagccgt ccacgcgctt
     7381 tacctatcgc tacgatggct cggccgtgca ggacttgtcc actgcctggg cggccctgcc
     7441 atcctcgccc gtgatcctga tcgccggcaa caagctttcc gccgatgcgt acgactccgc
     7501 ctggcgggtg ggtgtggcgc tggatcgcgc cggcaagcgg ccccggatca ggtccttgcc
     7561 ggctgtcggc gacgtggtcg atctgcgggg cgtggttgtg cccacggcgt tgcgcaggat
     7621 gcccgccttt gccgcgctgg ccgacggcgg caagcggcgg atcaaggacc tggccgagat
     7681 cggggcgctg atggcgttgg ggcaaggggg gcccttgcaa cccgacatcg tgatcggcga
     7741 ccgtgccacc ggcagcgtgc tggccgaggc cctggacgag ttgcgcgccc aactgccggc
     7801 cgaagtcatc gatgcctttt ccgaatggcg agggcacgcc ctggacggct ggggcaggca
     7861 gctggcggcc ggccaggtca ggctcaccaa tgtgttcggg cggcccgcca tcgtgatcgc
     7921 gccggacgcc ggcggccatg ccgcggccct gttcacccag tcctggcagc aggtcgcgat
     7981 ggggccgctg ctcaccgtgc acgcggccga cgagcagaag gccgatctgg cgacggtgtc
     8041 cctgaagtac ctgggcgcga agccggcgac cctggatgtc ctggcccgcg gcgactggaa
     8101 tgcgggtttc gacatcggcg cggtcgcgat ggacggccgc gggccgggga cactggtggt
     8161 ggacgttgcc gccgcgccgg gcgccgcgcg aactccgccg gtggtgtcgg tgttcctcaa
     8221 cgaggtgctg ctggccgcca aggagatgga ggccaatggc cggcgcgagc gcatcgtggc
     8281 gccgatccca cgccatgtac tgagcacgcg caacgacatc cgcgtgtcct tcgtgcgcca
     8341 gcaggccagc gaccgttgcc gcgagtcgcc cgaaccttac ccggtttcgg tgctttccag
     8401 cagccacatc gtcctggaca agatcgagcc catgggcgat ttcagcgggc tgatttcccg
     8461 cttcgccagt ggcgtgaacc tgctggtgcc gattgcctac ctgtacgacg cgcagaacac
     8521 gctgccgcgc gtgatcagtc tcgcgtccgc gaccggtgta gcgccggcac gcgcgcggtt
     8581 catcccggtc gccgatgtcg ggccgccgaa gatcaagggg ccgttcctgg ccatcgatgt
     8641 cgccctgaag gacgccgacc agagcgaggt caaggtggaa cgcggccgcc tgttcctgtc
     8701 ggacggatcc gagcgccccc tgctggatgt gagcggcctg aaccgggccg gcctgctgga
     8761 ggtcgcccgg gtcggccgcg atgcgggtgc gatctaccgc acgctgggcc gggacgcgcc
     8821 gaccatggac atacccatgc ggttatcggg cggcaacatt gcggtgatcg gaatggacgg
     8881 cctgcgcgcc gaggtgaaca ccgtcgaccc ctccggccag gggatggcgc gggaggcgcg
     8941 gccgtcgctg acggaccgcg gcttctggtg gctgctgccg ctgctggtgg tggctttcgt
     9001 cgccgcgctg ctcgcgtacg cttaccgcgt gcaccggcgc caggccaatg gctgaggcgc
     9061 ttgcctgtca agccgccgcg gcttgggcct gggcaacacg gaagcacagg tgcgagcaat
     9121 agtccaccag tgcgtcgatt tcggttgatt tgtcgaagac cgcgtccaca ccgagttgcg
     9181 cgcagcgctt gcgtacactg gcattgatgt agttgctgaa aacgactact ttctgattcg
     9241 gccggcgtcg cgacatgagc tgcgccaggt gcatgccgct gccttgcttc agaaacaggt
     9301 cgacgatcag caggtcccat cggtccatgt tcttatcgat ccaggcgagc gcttcatcct
     9361 cggtcgcgct cgttcctgcc accttcacgc aggtcagttc ctccagcgtg ccgaccagat
     9421 tttctcggat ggtcgggttg tcctcgacga cataggcttg caactccacg gggaaactca
     9481 tttcgtaaag gtttcgggat ttcagttact gtatgcacgt tgatctttgg atcgcaccgg
     9541 atgagcccgc atcctcgtgt aggacgcagc ctaccggcgc cgcccggcga tgcggccggg
     9601 cgcgcctaag atgtgaaatt gatggaccgc ccctcgatcg caatagaacc ggaccggctg
     9661 caacggaccg agagcttgct gaaactgctg gaacgcgccg cgttcattgg cgtgtggacg
     9721 ctcgatctcg agtcggaccg gctcgagtgg tccgaccagc tggcggcgat ccacgacgcc
     9781 gcgccgggct tcacgccgac gcgcgatgat gcattcgcgc tgtatgcgcc cgagtggcgg
     9841 gaactggtgc gggccctggt gcagggatgt gcgacggccg gcgagccttt cgacgaggaa
     9901 atgcagatcc tcaccttgcg tggccgacgg gcatgggtgc gtaccctggg gcatgcggtg
     9961 cgcgacgagg ccggcaacat cgtccggatc gaaggcgccg tgcaggaaat cgcgccccac
    10021 ggccaccgcg cgggaaccct gttgcgccac acggtcagca tggggggggc gatgggcggc
    10081 gtcgaagcgt tcgcgacgat cgaccgggaa gggcgcttga catatctgaa cgagcaggcg
    10141 gagctcctgc tgggcaaagg ggcgcccgag ctgctgggcc gcaagatctg gaactcgttc
    10201 cagaagacgg tgcggctgag gctggaggaa cagttcaggg acgcgttcga gtccgagaag
    10261 atcgtggagg tcgaagaact cgacgccaac ctttcgcgcc gcatcgaggc caggggctat
    10321 ccgttcggcg cgggcctggc cgtgcacctg cgcgatgtca cggcgcgcca caagtcgcag
    10381 gagcagctca ggctgctgga aagcagcatc gcgcgcctga acgacatcgt gatcatcacc
    10441 gaggcggggc cattcaacgc gcccgggccg cgcatcgtgt tcgtcaacga ggcgttcgag
    10501 cgccgtaccg gctactcgcc gcaagaggtg atggggcgaa cgccgcggct cctgcagggt
    10561 ccggccaccc agcgcgaaga actggaccgc atccgctcgg cgatggagca atggcagccc
    10621 gcgcgcgtgg acctgatcaa ctacaagaaa aacggcgaag ccttctgggt cgatctggag
    10681 gtgtcgcccg tgtgggacca cgagcgcaag ctgacgcact gggttgcggt gggccgcgac
    10741 atcaccgagc gcaagacggc cgaggaaaag atccagtacc tcgcgttcta cgacccgctg
    10801 acccggctgc ccaatcgcca gttgctgctg gaccggctgc agcgcgcgct ttccgaccag
    10861 gaccgcccgc gcgagggtgc gctgatgttc atcgacctgg acaacttcaa ggtcctcaac
    10921 gacacgatgg gccaccacaa gggcgacctg ctgctgcagc aggtggccga gcgcctgcgc
    10981 tcctgcgtgg cgcgcggcga caccgtggcc cggctggggg gcgacgaatt cgtgatcctg
    11041 ctcgaaaaca ccgggcacaa gcccctggag ccggccgccg gcgcgcggat cgtcagcgag
    11101 cgcatccttg cccggctggg tgagacgtac ctgctggccg gccatctgca ccacagcacc
    11161 tgcagcatcg gcgtcacatt gttcggccgc gcgccgtgga cggtgagcga actgctcaag
    11221 caggccgacc tcgcgatgta ccaggccaag ggcgcgggac gcaacgccgt gcgtttcttc
    11281 gacccggaaa tgcaggcggt ggtgaccgcg aacgcggcgc tggcgaccga cctgcgcgag
    11341 gcctggcgcg agagccagtt cctcatcgac taccagcccc aggtcggcgc cgacgggcgg
    11401 atgaccggcg tggaggcgct gctgcgctgg cggcacccgc agcgcgacat ggtgcccccg
    11461 gcgcatttca tctccaccgc cgaagagact tcgctgatca tcccgatcgg gcactgggtc
    11521 ctggagcagg cctgcgcgca actggcgcag tgggcgacga ggcccgagcg gcggcacctg
    11581 agcattgccg tcaatgtcag cgtgcgccag tttcgccatc ccgatttcgt ggacgaggtc
    11641 atgaccgcca tcgagaactc gggcatcgcg ccgcacaagc tcaagctgga gctgaccgaa
    11701 agcctgctgg cggacggcat cgaagtgaca gtggccaaga tggggaggct caaggagatg
    11761 ggcgtcacgc tgtcgctgga cgacttcggc atgggctact cctcgctgtc ctacctcaag
    11821 cggctgccgc tggaccagct gaagatcgac cgcgagttcg tcaaggacat cctcaccgac
    11881 gccaacgatg ccgccatcgc gcgcaccatc gtcgggctgg cccagagcct gggcctcggg
    11941 gtcatcgcgg aaggggtgga aacgcaggcc cagcgcgctt tcctggcgca gcagggctgc
    12001 tatgcgtacc agggcttcct gttctgcagg ccgctgccga tcgagcagct cgaggtcttc
    12061 atggacgagg tcgaaccctg aagcggcggg ggtccgccgg gggtcagatc ggcgcgatgc
    12121 gcaagggcgc ggttgccacg gcacgcggca tccgcatcac gatctggtcg ggtccacgta
    12181 ccagctccac cgattcggcg accgccaggg cctgcacgtc ggaccagtcc agcaggcggt
    12241 agctgggccc gaacggcagg gcctcgaact cgtcctgccg gggcgtcaca cgcacggaga
    12301 tctcggcgaa tccgccctgc acctcggctt tcacccgtac ttcggacggc cccggggccg
    12361 cgtccgtcag ggcgaggagc gacgccatca gcaggttgcg cagggtgacg cgccagacct
    12421 cgaaatcggt gtcgggtact tcactgacga tggagaaacc gcggaagttg aaattggtgc
    12481 tcagcagggc gaggcaatcc tcgatacccc gggccagtcg aacgccctcg tcttcccccg
    12541 gctcgatcca tgcggccacc ttcaggcagt tcatgacggc atcgcgcgcc aggcggttga
    12601 gtttggaaat gctggactgg aagtcggcgg gcgcggaaga gccgcgctcc agccgggcgc
    12661 tcatcacctc ggccatcatc gagatagcct gcaggttgac caccatgtcg tgcttgagcg
    12721 ccggcgcgag ccggcgcagc acgccatagc gcgcggcttc ggccccggcg acgcgcggcc
    12781 tgcgcgctac gctcgcgctg ccttcgccgt ggtgcgcatc ggccactagc ggcatgggca
    12841 ccacctcgcc cgtgccccag cgcccctgcc ggccttgcat ctccagatca tcttcggcac
    12901 ctcttgtgtc gcgcttcacg ttgccttcct gctgtgtcgg gtcgcgtccc ggtacgtttc
    12961 gtgcaaagct tgccgccggc cgggcaacgg ctcgcgcagg cccgttgggg taaggaaacg
    13021 gacggaatcg cggctaccca taacttctcc tgagtatggc tcatgttcca cgatccgtgt
    13081 atcggggcct gtaggagaag tctcatgcat ttgtaggata aaaccagggc ggcgaacgcc
    13141 cgagcgccgg tatgctcgtg gaatgagcaa ggcattcacg agggaacccg acgtcgacga
    13201 cgaggaagag gcctcgttac cggagctcgg gagcggcggc aagaactaca tcacgccgca
    13261 aggctacgcg cggctgcgcg ccgaactgct gcagctgatg gacgaagagc ggcccaaggt
    13321 ggtcgaggcg gtgcactggg cggccctcaa cggcgaccgg tccgagaacg gcgactacat
    13381 ctacggcaag aagcgcctgc gcgagatcga ccggcgcatt catttcctca cccaccggct
    13441 ggaaatcgcg gaggtgaccg acccggccgt ccatcatggc ggcgaccagg tgttcttcgg
    13501 cgccagcgtc acctacgccg aggagaccgg ccaggagcgc actgtcacca tcctgggcat
    13561 cgacgaggcg gacagcgccc atggccaggt cagctggatc tcgccgatcg cgcgggccct
    13621 gctcaaggcg cgcgtgggcg acgtggtcaa gctggtcact cccgcgggca cccacgagat
    13681 cgaagtcctg cgcgtgaact atcccgcacc ggcggccggg ccggatcgcg acacccgcgg
    13741 gtagggaagc ggcggatcag aaggtgctgc cgggccgggc ccgctggacc cggatcaccg
    13801 agggcgtgcg cttcagggcc cgcatcgccg tctcgaggtg ggcgcggtcg cgcaccgcga
    13861 cgatgaagcg caggtcggtc gcgtcctgcg ccttttcgtc tcccatgtcc acatgcgtga
    13921 tatcggcctc ggcgccggcg agcgcggcgg caacccgcgc cagcacaccc ttgccgttct
    13981 ccaccgacac cagcaggccg gtttcgaacg gccgcaccgg ctcgtcggcc cattcgacgc
    14041 tgatgaagcg ttcgctgtcc ttgtgctgca gccgcttggc gacggcgcag tcctcggtgt
    14101 gcaccaccag gccctcgccg cgtcccaggt agccgacgat ggcgtcgccc ggcaccggcc
    14161 ggcagcaggt ggcgaactgc acggacgcgt tctcgctgcc gtccaggatg atttcgccct
    14221 gcgacacgtt ctcgtgcgcg gtatagcgct ctcgcgtgat cagcagcgcg tcgggtttct
    14281 cgccgcgttc ggccagcatg gtcaccagcc gcttggccac gatgctcgcg atgcgcttgc
    14341 ccagcccgat gtcggtgagc agttcggcgc ggctgcggtt gccggtgaag cgcagcagct
    14401 tttcccagat cgggcgatgt tcctcgtcgt ccgccggcag cttttccagt ccttcggcac
    14461 gcagcgcctg cgtcagcaat ttctggccga gctccagcga ttcggcctgg gccatcgact
    14521 tcaggtggtg cctgatcttg gagcgggcgc ggccggtgcg aacgaacccg agccaggccg
    14581 ggttgggcgt ggacaccggc gccgtcacca cttcgaccac gtcgccgttc ttcaactcgg
    14641 tgcgcaaggg cacctgcagc ccgttgatct tggccgcgac ggtgcggtcg ccgatgtcgc
    14701 tgtgaatggc gtaggcgaaa tcgaccaccg tcgcgccgcg gggcagggcc atgatctggc
    14761 tcttgggcgt gaacacgtag accgcgtccg ggaacaggtc gatcttgacg tggtcccaga
    14821 actccgccgc gtcgcgcgtc tcgtcctgga tgtccaacag cgactgcagc cacttggtgc
    14881 ccagccggtc gccgccttcg ccttcggggc cgctggcctt gtacagccag tgcgcggcga
    14941 cgcccgattc ggcgaccacg tgcatggcct cggtacgcat ctggaattcg acgttcacgc
    15001 ccgaggggcc caccagcgtg gtgtgcagag actgatagcc gttgaccttc gggatggcga
    15061 tatggtcctt gaacttgccg ggcaccggtt tgtacatctg gtgcaggacc ccgagcgccg
    15121 tgtagcagtc ggtgacggtg ggcacgatca cccggaagcc gtagatatcg gtcacctgcg
    15181 cgaagctcag gtgcttctcg tccatcttgc ggtagaccga gtacagcgtc ttttcgcggc
    15241 cggcgatccg cacgttcatg ccggccgtct cgaacgcgct ttccacttcg cgctgtacct
    15301 tctggatcag gtcgcggcgg cggttgcgcg ccttcgtgac ggccttggtc aggatggcgt
    15361 ggcgccaggg ccgcaggtgg cggaaggcca gttcctgcag ctcgcggtaa gtctggttca
    15421 gccccaggcg atgggcgatg ggcgcataga tatccagtgt ctccgacgag atgcgcgccc
    15481 atttctcgcg cggcacgtcc accagcgtgc gcatgttgtg gctgcggtcc gcgagcttga
    15541 tcaggatgac gcgcacgtcg cgcgccatcg ccagcagcat cttgcggaac gactcggcct
    15601 ggctttcctc gcgggtgtcg aactgcagcc ggtcgagctt ggtcaggccg tccaccagtt
    15661 cggcgaccgg cgcgccgaag cgctcgatca gctggggctt ggtgacgccg cagtcctcga
    15721 tcgcgtcgtg cagcagtgcc gccatgagtg cctgcgcgtc cagcttccat tccgcgcatt
    15781 gcgcggccac cgcgatcgga tgggtgatgt agggttcgcc gctggcccgg atctgcccca
    15841 ggtgggcttc gtcggcgaag cggtaggcct tgcggacctg ctcggcttcg gcggggtcca
    15901 ggtagtcgag cctggcggtg agggcagcga agctcgccgc ctcggcgttg gccgcggccg
    15961 ggctggccgc ggcttccgcc atcgggcgat tgcctttgcc ggacgaggat tgcagcaccg
    16021 cgctcataag acccccacgc ttgcgggcat gcccgcagcg ctcggctcgc gcaacgtccg
    16081 tgcgcggagc tgtacagtgg agcgagccga tgccgggcct gcgctatgca cgtttcgagg
    16141 cataacgctg cggcccggcc ccgagggggt gcaagcttgc ttgaagcggt tctgcgcggc
    16201 gctcatacct taaatgtagc gcgcgcgtgc tccaggtgtg cacagcaaca aaaaagcacc
    16261 gcatggcggt gctttagtag ggcaaaagac ctgtttgaag gctcagccgg gcactttctt
    16321 gagcatttcc aggccgatct tgccttgggc gatctcgcgc agggccgtga cgccgggctt
    16381 gttcttgctt tcgatcttgg gcgcatggcc ctggctcagc atgcgggcgc ggtaggtggc
    16441 ggccagaacg agctggaagc ggttggggat gtgctgcagg cagtcttcga cggtgatgcg
    16501 ggccatgaat gttctccagc gattgatgaa tcgactccct ggggagtcac gtgatgttga
    16561 gggccttgaa ggtatcggcg cgggcacggc gctgggccga aaatttgagc cgttgggcgt
    16621 gaacgaccgc tttcaggtcg aacaacgcgc gatcaaataa ctcgttgatt ataacgaaat
    16681 cgaattccct gacctgcgca atttcttcgg cggcgttctt caggcgcatg tcgatgactt
    16741 cggccgagtc ctcgccgcgc cgttccaggc gggaccgcag ttcctcccag ctcgggggca
    16801 ggatgaagat cagcaccgcg ttgccgaaga tgcgcttgat ctgcagggcg ccctggaaat
    16861 cgatctccag gatcacgtcg gcgccctggg cgatgcgctc ctcgatggct ttcttggacg
    16921 tgccgtagcg atgcccgtgg acgaaagccc actcgacaaa ggcatcggcg agcaccattg
    16981 cgtcgaactc cgggggcgaa acgaagaaat actcgcgacc gtgcttttcc tggccgcgcg
    17041 gcggccgggt cgtgtgcgag accgaaggct gtacccgcgc atccagctcc atcagcgcgt
    17101 tgaccaggct ggacttgccg gccccgctgg gagcggcgac gacgaacagg ttgccgggat
    17161 agtccatgtg gtcgccgttt tactcgatgt tctggacctg aagctcgata tcggccgcaa
    17221 ggtcttgatt cttatggatg tcgcactccc gggtaggtgc cgcaccccac gatttacccc
    17281 acagaaatcc tgggctgctg tgagtcgtca tgaggcgatc gcgtgtcggc aatttcaacg
    17341 tcgctacgag ccgttatctt atcagtcgga cttttcgtga gcttgttgtc gcgtgggcgg
    17401 aaaacccgtg gcttgcccca ctcgacggca agcgggactg actggaacgg caggcaatgg
    17461 gccggtgtgt cagactcgtc ttccatgacc gacgacgagt tcccgtacag ccgctcgctt
    17521 gccccgttgg aatcgctgct cgcgggcgtg aagcggcctg gcgacttctg cgtgcacggg
    17581 gctctggcgt tgccgatgcc aatggtccag gtggacggcg tcggcttgct gtcgtttccg
    17641 cttccgcctg cgcagattgc ggccctgatt gctgccgctg agcgcgcacc ctacgggcgc
    17701 ggcgaacaga ccgtggtcga cacgtcggtg cgccgggtct ggcagatcga cgcggcgcgg
    17761 gtgcggatca gcggcaagag ctggacgcaa tcgctgggcc ggatcctcgc cgagactggg
    17821 cgcggactcg gctgcgagga caccgcgatt caagccgagc tgtacaagct gctggtgtac
    17881 gacccgggcg gcttctttct tgcgcaccgc gacagcgaga aggcgcccgg catgttcggc
    17941 acgctgctgc tgcaactgcc atcgccgcat tcgggcggcg agctgcgcat ccgccacggc
    18001 agccgcgagg cgcgcatcga tctgtcggcc accgactgct ccgagttcgg cttcgccgcc
    18061 ttttatgccg attgcgagca cgaggtgcgg ccggtcgaat cggggcaccg ggtctgcctg
    18121 gtcttcaacc tgctgcgcgg cggcgcgagc gacacgccgc cggccagtgc gccgagccac
    18181 gacgccgagg tcgcgcaggc ggcatggctg atcggcgcgg ccttcgccgc cgagggtgcg
    18241 ccgcgcaagc tggtctggct gctggcgcac cagtacagcc aggccgagct gcgcctcgcc
    18301 gcgctcaagg gcgccgacgc ggcggtggcc ggggtgctgt gccgggcggc ggaggcggcg
    18361 gcctgccgcg cgcatctggg cgtggtgcac atcgaggagt ccgggccggc ggaggtgcac
    18421 tacgcgccca gtcggggtcg ccgctaccac gacttcggcg acttcgacga cattgatgcc
    18481 acgggggccg attacgaggt cgtcgaaatc tccgacacca cccgctacat cgatcactgg
    18541 gtcgcgcctg gcgaccagaa acccgcgctc ggccggttgc cgctgggacc cggcgaactg
    18601 ctgcccgccg gcgcgctgga cgacgaacca ccggatgagg accgcctgct ggagtccacc
    18661 ggcaacgagg gggttagttt cgagcgcgcg taccggcgcg ccgcgctggt cctgtggccg
    18721 cgggcgcggc aggtcgaggt gctgctgcaa gccggggtcg ccgcggcgtt gcccatgctc
    18781 gaggaggcgc tcgcggccga cgcagcggac gctggcgcgg cggcgttgga tgtggccgag
    18841 cgtatcgtgg cccattggtc gcggcaaagc cccccgcgct acgccgccgc gcgggaaggc
    18901 ccgctgcgtg cccgcatgct gcgctgtctg gcccggttgg gcacgacggc ggcgggcgaa
    18961 cgtttcctgc gcgaggtgct ggtcgccgac tacgacggca gcgagaacgc cgcccttgcc
    19021 gaggccgcgc cggccatgct ggcggcccag cccctccagg aactgctcgc tgcgctggtc
    19081 gaatcggcgt tcctgcgctg cccgcgcgcg ctgtgcgaac tgctgttcct gctggacgca
    19141 ggcaaccagg cgcagcccag cctggccggc gacttggcgt tgcgccgctt tgcgggggcc
    19201 ttggtcgatg ccctgccggg gctcgcggcc cgaccgtcac gcagcgacct tggctgggtg
    19261 ctgcagcacg acgaaaccgc cgccgacgcc gaaagctgcc accgcctgct gggggtgctg
    19321 gaacggctgg cgagcggaga gcatcacgca gcagcagtca ccaccttgat cgcgcagccc
    19381 caggtcttcg accccggccg ggttctggtg ccggcgctgc aagctttgtg cgagatcacc
    19441 cagggcctga agagccgcgc cggtgcggag cgcggcggca tcgatcgcga tgcccaacgc
    19501 ttgcggctgt ggcgccattg ctgcgcctta ctgctggcgc gcagcgagtt cgcgcccccg
    19561 gcgccgcgcg actggagcca gccggctgtg ctggcctgcc gttgcgagga ttgccgggcg
    19621 ctgcaggcct tcgcgctgga tccgcggctg cgcgagcagc gctttcgcgt ccgccaggac
    19681 cggcgcgagc atctgcaacg ccagatcgcg cagcacgggc tggacatgca ccacgtcacc
    19741 gatcgcagcg gttcgccgca gaccctcgtg tgccgcaaga cgcgcgagag ttaccgccgc
    19801 cagtgccgcc agcacgccga ggacattgcc gccatgcgcg ccctgcaggc gctggccgtc
    19861 gccgcgccga atgcgcagga aagcgctcgg ctcgcggcag cggtggcgcg ccagccgcag
    19921 gcggtggagg gctgaccctt ggccttccat gcagcagagg ctcagcctgc gcctgcatcg
    19981 gcgacgctac attggcagcc gaaccacgtt gacctgagtt cgaccgcgcg ggcgaaggtc
    20041 gcactgttcc gatcgctttt tcgtggccgg gatgatctct atgcgcgccg cttcgaaagc
    20101 gcgacgacgg gcaagtccgg ctacgcgccc gcctgcgcca gtgaatgggt gcccggtatc
    20161 tgccgcaagc cccggatcaa gtgtgcggat tgcgctcatc gggcgtttct cccgctgact
    20221 gatcaggtcg tctattggca cttgtgcggt catgcgcccg gcgagccgcc gcaccgcccc
    20281 ttcgttgcag gcgtctatcc gctgcgcgag gacgagacct gcttctttct tgccgccgac
    20341 ttcgacaagg ccggctggca agacgacgcc gcggcgtttg tccaggcgtg ccgccggctg
    20401 catctgcctg tggcgctgga gcgatcacgc tccggggaag gcggccacgt ctggctcttc
    20461 ttcgagactg ccctgccggc tgcaatggca cggcggctcg ggtcccacct cctgacagaa
    20521 gcgatggagg cccgccccga catcggcctg gattcgtacg accggctctt tccaagtcag
    20581 gacacgatgc cccgcggcgg gtttggcaac ctcatcgccc tgccattcca aaaggaaccg
    20641 cgcgagctgg gcaacagcgt gttcctgaat gacggcttca tgccgtgggc cgaccagtgg
    20701 gcatttctcg cgaacgtggg caagataggg cgggcacagg tcgaaggcat cgtccgcgaa
    20761 gccgagcaac gcgggcgcat cctgggcgtg cgcctgccga tgcaggagga aggggaagac
    20821 gagccgtgga ccctgcctgc ctcgcgccgg cgcaaggagc cgccgctcgc gggcgagttg
    20881 ccgccgacgc tggaactggt tctcgccgac cagatctaca ttgcccggca aggattgccc
    20941 cccggtctga tgaaccgtct gatgcgcctg gcggcctttc agaaccccga tttctaccgg
    21001 gcgcaggcca tgcggctttc cacctacggc aagccgcgtg tcatcacctg cgccgaggat
    21061 catccgcttc acctcgcgct gccgcgcggt tgcctggacg atgtgcgccg gaccttgaag
    21121 gacctgggcg tgcgcgtgac gctgcgcgac gagcgctgca gcggccagcc tttggatttg
    21181 cgctttcatg gtgaactgcg gccggagcaa acgtcggctg cacaggcgct actggcgcat
    21241 cagaccggag tgctcgccgc cacaaccgcc ttcggcaaga ccgtggtcgc ggcctggctc
    21301 atcgcgcagc gcggcgtgaa cacgctggtg ctggtgcacc ggcagcagtt gctcgaccag
    21361 tggatcgcgc ggctgtcggc tttcctcgac ctgcccgcca acgcgatagg ccgcatcggc
    21421 ggcggacgac gacggccgaa cgggcggatc gacgtcgccc tcattcagag cctcgtcaac
    21481 aaaggcgtcg tcgcagacga ggtcgccaac tattatggcc aactgatcgt cgacgagtgc
//
LOCUS       DASWIY010000297         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_934086, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000297 DASWIY010000000
VERSION     DASWIY010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934086"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            538..1170
                     /locus_tag="VGA59_10970"
     CDS             538..1170
                     /locus_tag="VGA59_10970"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX9720243.1"
                     /translation="MLFLGAVAVAIGAFLLLRVISIHEMIERIGALGPLPFFLALAVL
                     PAFGAPVTPFYLLAGASFGTTAAIPIVLGGLAGNVALSYVMARWVVCPLIVRIVKHLG
                     YDIPVVRPEDRWNITLLVRVTPGPPFPVQSYVLGLAGVPFSIYMVISMLVASVFAIGM
                     IFFGESLMHGSSGLLVAGVSMLIAAFVAIRLVRGMVQRRAARASATNPAA"
     gene            complement(1267..>1952)
                     /locus_tag="VGA59_10975"
     CDS             complement(1267..>1952)
                     /locus_tag="VGA59_10975"
                     /inference="COORDINATES: protein motif:HMM:NF018749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HEX9720244.1"
                     /translation="AVTSHNTSTLTTATMTNFAIAGSAPPTTSGTPGGTTSPPGGTSA
                     TPLSQLDIGSPLLAGSTSFDGNSGIYYMKAAGNDIWGTADQFQFAYRQVSGNCTLIAR
                     FDSLSPTNIWAKAGLMIRGSLTGGSAHASLFVTPGVGVVQQARVSTNGESISTSPAPG
                     IAPQWLKLVRNGSAFTSYRSSNGSTWDYIDSFTVSLPNSVYVGLAATSHEAATATNAI
                     FSNLSITSP"
BASE COUNT          327 a          529 c          696 g          400 t
ORIGIN      
        1 tcggtcgtgg tttgccgttg aaccggcaag ccagcgctgg agaaaggcgg caagaaacag
       61 cgcggagagg agcggaacgg ctgcgccgag gctaatttca gggtgtctgc tgaacacagc
      121 ggcgaccgcg agggcaagac ccatcgcggt gagcgcagcg ctgaaaagcg gagaggatcc
      181 cagctccgag gcgcggttgt cggcctgtcg cggagcgacg agacagagga agacaggaac
      241 gagaagggcg gattgccaga gcagccacca cggccaagcg cacatgcgtg tggccccgct
      301 tggcgagaga gaaagtgctg ccagcgagag agttccgaag cttgtcgcaa tcacggcaag
      361 cgagcggcgc cacccatgac cggggctggc cggatggggc gcggctgggc gcatgggtgt
      421 tgcaggcagt cgcgcggcgc gcattgtcag ggtttttgcc ttggtttgcg cgccaatgca
      481 tgatgaatcc gcgtcgtggc tgttaggatt cccaagctga agtcgtggca gtggggcgtg
      541 ctcttcctcg gcgcggtggc cgtggctatc ggcgcctttc tgctcctgcg ggttatttcc
      601 attcacgaga tgattgaacg gatcggcgcc ctcgggcctt tgccattctt tctcgcgctt
      661 gctgttttgc ctgcgtttgg cgcaccggtg acaccgtttt atctcctggc cggggcaagt
      721 tttggaacga cggctgccat acccatcgtg ttgggaggac tggcggggaa tgtggcgctg
      781 tcgtacgtca tggcgcgctg ggtagtgtgt ccgttgatcg tccggatcgt gaagcacctg
      841 ggctacgaca tcccggtggt tcgtccggag gaccgatgga atatcaccct gctcgtgcga
      901 gtcacgccgg gaccaccctt cccggtgcag agctacgtcc tgggtctggc gggagttcct
      961 ttcagcattt acatggtcat ctcgatgctg gttgcttcgg tttttgcgat tggcatgatc
     1021 ttttttggcg agagcctcat gcacggcagc tccgggctgc tggtcgcagg agtgtccatg
     1081 ttgatcgccg cgtttgtcgc gatccggctg gttcgcggca tggttcagcg gcgggcggct
     1141 cgcgcgagcg cgacgaatcc ggcggcctga ggctggcggg tcgctcacga gagatacgcc
     1201 aagaatgaaa gcacgcggcc ggtgggccgc gtgctccctg ggtttgtcgt gcgctggtgt
     1261 ctggtcttac gggctcgtga tcgacaggtt gctgaaaatc gcgttcgtcg ctgttgctgc
     1321 ttcgtgggat gtggccgcaa gacccacata aacgctgttc ggcagggaaa cagtgaaact
     1381 atcgatgtaa tcccatgtgc tcccgttgct cgaccggtag ctggtgaaag ccgagccgtt
     1441 gcgcacgagt ttcaaccact gcggggcaat cccgggggcg ggggaggtcg agatggactc
     1501 gccgttggtt gatacccgcg cctgctggac cacgccgacg ccgggagtga cgaacagact
     1561 tgcgtgagcc gaccctccgg tgagggagcc gcggatcatc aagcctgcct ttgcccagat
     1621 gtttgtgggc gagagtgagt cgaagcgggc gatcagcgtg cagttgccgg agacctgccg
     1681 gtaggcgaat tgaaactggt ctgcggtgcc ccagatgtcg tttccagcag ccttcatgta
     1741 atagataccg ctgttgccgt cgaacgaagt ggatccggcg agcaacgggc ttccgatgtc
     1801 gagctgggac aggggcgtcg cggaggtgcc acccgggggc gaggtcgttc ctcccggggt
     1861 tcccgatgtc gtcggcggag ccgaaccggc aatggcgaag ttggtcatcg tcgccgtggt
     1921 gagagtggat gtattgtggg acgtcaccgc ga
//
LOCUS       DASWIY010000298         8682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_578027, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000298 DASWIY010000000
VERSION     DASWIY010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8682
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578027"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VGA59_10980"
     CDS             <1..485
                     /locus_tag="VGA59_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720245.1"
                     /translation="FENLRCGVLWSLGYRYTFLKFLADLHDANDRAEQLLDMIKLHRK
                     RDVLAMNLTYAEQRALEIGITIAGGANVILLDEPTAGMSKSETRRFIELIKEVTVGKT
                     LLTVEHDMGVVFGLADKIAVVVYGEVIAFDRPEAVRANARVQEAYLGSHVADAQVEAR
                     "
     gene            485..1180
                     /locus_tag="VGA59_10985"
     CDS             485..1180
                     /locus_tag="VGA59_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720246.1"
                     /translation="MLTIDNLHAFYGKSHVLHGVHFQVGQGEIVALLGRNGSGRSTTA
                     KSIMGMVECTGTVDWKGRHTLGMKPYEIAHLGIGYVPENRDIFPKLTVHQNLMLGQKS
                     AQQKGRWAFDDMYAMFPRLKERQHTEAGVLSGGEQQMLTLCRTLMGDPDLIIIDEPTE
                     GLAPKIVELVAEYLQKLKQRGISVLLIEQKLTIAMAISDRALVMGHGSIVFQGTPDSL
                     RADAYIRKEWLEV"
     gene            1410..3509
                     /locus_tag="VGA59_10990"
     CDS             1410..3509
                     /locus_tag="VGA59_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9720247.1"
                     /translation="MTAEYKVHGDVAVITMNNPPVNGLGHATRLGLASGLDQANADAS
                     VKAVVITGAGKAFSGGADINEFGSPKSYAEPNLHSVILAIENSAKPVVAAVHSVAMGG
                     GLELALGCHYRIAAPGTNVALPEVKLGLLPGAGGTQRLPRVLGAETALNMIVSGESVK
                     SELLAQQPGQKLFDRMAASPESLMEEAISFARSVADTRPLPLVRNLPCKHPLGDAYFQ
                     FARNMVKGMAKNFPAPAKCVDAVEAATKKKFDDGLALEREIFLNLMLTPESRALRHVF
                     FAERATSKIPDVPAETPQREIKSVAVIGAGTMGGGISMNFLNAGIPVKILEMKQEALD
                     RGIATIRKNYESQVKKGKLKVDKYEQRMSLLSTTLDYNDLKDADLVIEAVFEEMGVKQ
                     KVFEKLDEVGKPGAILASNTSTLDVNKIASFTKRPQDVVGLHFFSPANVMKLLEVVRG
                     AKTGKDVMATVMTLAKKIKKTAVVSGVCDGFIGNRMVEQYSRQAGFLLDEGATPQQVD
                     KAIEKFGFAMGPFRMGDLAGNDIGWAIRKRRAQERPNMLYSRTADKLCELGRFGQKTG
                     AGWYDYQAGKRDAIPSELVNKMIEDHRKELGITPRKISDEEIVQRLVFALVNEGARIL
                     EDGIASRASDIDMVYLTGYGFPLHRGGPMLYADQVGLFNVMQAMKRFQRNPHDDAKFW
                     EPAPLLARLAAEGKNFN"
     gene            3613..4788
                     /locus_tag="VGA59_10995"
     CDS             3613..4788
                     /locus_tag="VGA59_10995"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX9720248.1"
                     /translation="MSNAVIVSTARTPLAKSWKGSFNMTHGATLGGHVVKHALQRAGI
                     DAAAVEDVIMGCANPEGATGANIARQIAIMAGCPVTTAGMTVNRFCSSGLQTIALASQ
                     RVIAGEGEVFVAGGVESISCVQQEMNTHMMKDPDLDKLKPEIYWSMLQTAEQVAKRYK
                     IERDVMDEYGAVSQQKACAAQAEGRFDAEIAPITVKAGVADPVMGLRTKEVTVSKDEG
                     TREGTTKEGIGGIKPALPGGVIAAGNASQFSDGAGACVVVSEEYAARNNLKPLGRFLG
                     FAVAGCEPDEMGIGPVFAVPKVLKKLGLKVSDIDLWELNEAFAVQVIYCRDRLGIPND
                     RLNVNGGAIAVGHPYGVSGQRLTGHALIEGKRRGAKRVLVTMCIGGGMGAAGVFEVL"
     gene            5023..6849
                     /locus_tag="VGA59_11000"
     CDS             5023..6849
                     /locus_tag="VGA59_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9720249.1"
                     /translation="MSLRPTLNFLLYDWLNAEELTARSRFADHSRETFDAVFDTCERI
                     AREKYAPFNRTVDTEEPRFDGEKVILPKATHEAHKAFAGSGMLSAAQDYEIGGMQLPY
                     TVEAASHTFFAMASASIGSGMLTVGNANLLMAHGTEPQKTVFALNEFSGRWSGTMCLS
                     EPQAGSSLSDITTRAVPDGDGFEGDPLGPRYRLRGNKMWISAGEHELTENIIHLVLAK
                     IPGPDGKLIAGTRGISLFIVPKMLVNERGELTGERNDVALAGLNHKLGWRGTTNTLLN
                     FGEGKFPVDGQAGAVGYLVGRPHEGLRCMFHMMNEARIGVGMAAVMLGMAGYHASLDY
                     AKNRPQGRPVGPRGKDPAQPQVRIIEHADVKRMLLAQKSYCEGALALELYCARLVDEQ
                     HTGEPQAADEARLLLEVLTPIAKSWPSEWCLEANSLAIQVHGGYGYTRDFPVEQYWRD
                     NRLNMIHEGTHGIQAADLLGRKVLMEGGRGLQLLAGRIGATIERARQHSRFPEEARAL
                     DQALRDVQAATQAAWSTGDPAQALANAVPYMQAFGHMVLAWIWLDVVLATPEAASGEP
                     AHQGRLRAARYFYRYELPKIGAWLNVAATRDETCAGMPEEAF"
     gene            6961..7752
                     /locus_tag="VGA59_11005"
     CDS             6961..7752
                     /locus_tag="VGA59_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9720250.1"
                     /translation="MTRSVSQLFDLKGRTALVTGGSRGLGLQMAHALGEAGARVMLTS
                     RKADDLEQAAAELQSAGIDARWIAADCGKESDIHRLADETLQRMGDVDILVNNAGAAW
                     GAPAEDHPVEAWDKLMNLNVRGYFILSQRIAKRSMIPRKYGRIVNIASIAGLGGNPPE
                     MQTLAYNTSKGAVINFTRALGCEWGKYNITVNAICPGFFPSKMTKATFERLGEAELAA
                     HAPLQRLGDDEDLKGVTLLFASEAGKHITGQWLAVDGGVSVVTGG"
     gene            7765..8193
                     /locus_tag="VGA59_11010"
     CDS             7765..8193
                     /locus_tag="VGA59_11010"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9720251.1"
                     /translation="MQFGVDIPFVHHLGFELVQFEGGRSEIQYEARPEHLNSFAVTHG
                     GACMTLLDVTMAAAARSVDKNMGVVTIEMKTSFMQAARGKLAGRGRLMHRTATMAFTE
                     ATIYDGEGRACAHATGTFKYVKRLPTGAKSVLPLNIISTD"
     gene            8290..>8682
                     /locus_tag="VGA59_11015"
     CDS             8290..>8682
                     /locus_tag="VGA59_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX9720252.1"
                     /translation="MPQNKQFLLDNRPQGEATAGNFKLVTGETPPLQDGQVLVRHHFL
                     SLDPYMRGRMNDAKSYAAPQPLGQVMQGGTAGEVVESRSPKYQPGDKVVGSGGWQEYS
                     VVDADQPGALRKVDTTQVPLSHYLGAVGM"
BASE COUNT         1688 a         2733 c         2896 g         1365 t
ORIGIN      
        1 tcttcgagaa cctgcgctgc ggtgtgctct ggagcctggg ctaccggtac acgttcctga
       61 agttcctcgc cgacctgcat gatgcgaacg accgcgccga acagctgttg gacatgatca
      121 agctgcaccg caagcgcgac gtgctggcca tgaacctgac ctatgcggag cagcgtgcgc
      181 tggagatagg catcaccatc gccggcggcg ccaacgtgat cctgctcgac gaaccgaccg
      241 ccggcatgag caagagcgag acccggcgct tcatcgagct aatcaaggag gtcaccgtgg
      301 gcaagacgct gctgacggtc gagcacgaca tgggcgtggt cttcggcctg gccgacaaga
      361 tcgccgtggt cgtttacggc gaggtgatcg ccttcgaccg cccggaggcc gtgcgcgcca
      421 atgcccgcgt gcaggaggca tacctcggtt cccatgtcgc cgacgcccag gtggaagctc
      481 gctgatgctg acaatcgaca acctccacgc cttctacggc aagagccacg tgctgcacgg
      541 cgtgcacttc caggtgggcc agggcgagat cgtcgcgctg ctgggccgca acggctccgg
      601 ccgttcgacc accgccaaat cgatcatggg catggtcgag tgcaccggaa cggtggactg
      661 gaagggccgg cacacgctgg gcatgaaacc gtacgagatt gcccacctgg gcatcgggta
      721 cgtgcccgag aaccgcgaca tcttccccaa gctgaccgtg caccagaacc tgatgctggg
      781 ccagaagagc gcccagcaaa aagggcgctg ggcgtttgat gacatgtacg cgatgttccc
      841 ccgcctgaag gaaaggcagc acaccgaggc cggcgtgctg tcgggcggcg agcagcagat
      901 gctgacgctg tgccggacgt tgatgggaga tcccgacctg atcatcatcg acgagcccac
      961 ggaaggcctg gcgccgaaaa ttgtcgagct ggtggccgag tacctgcaga agctcaagca
     1021 gcgcggcatc tcggtgctgc tgatcgagca gaagctcacg atcgcgatgg cgatttccga
     1081 tcgcgcgctc gtcatggggc acggcagcat cgtgtttcaa ggcacgcctg acagcctgcg
     1141 cgccgacgcc tatattcgca aggagtggct ggaagtgtga tcacttcaaa aacgctgcgc
     1201 gttttgtgaa gtggattgaa cagatgaaga aatgcggcaa agccgcattt cttcattggg
     1261 gccgcactgc acgttgttac gtgcggccgg tgcccctgcg gggagtttgt cccggtggag
     1321 acaaagggcg tgctgcgatc agcgatacca ctctagaatc tggaaaagaa cgaccgtacg
     1381 tttttcgata aatcggtgaa ggaaccaaga tgaccgctga atacaaggtg cacggcgacg
     1441 tggccgtgat tacgatgaac aatccgcccg tcaacgggct ggggcacgcg acccgcctgg
     1501 ggctggccag cggcctggac caggccaacg ccgacgcgtc ggtgaaggcc gtcgtcatca
     1561 cgggcgccgg caaggcattc tccggcggcg ccgacatcaa tgaattcggc tcgcccaaat
     1621 cctacgccga gcccaacctg cactcggtga tcctcgcgat cgagaattcg gccaagcccg
     1681 tcgtggccgc cgtgcattcg gtcgccatgg gcggcgggct cgaactcgca ctgggctgcc
     1741 attaccgcat cgccgcgccc ggaacgaacg tcgccctgcc cgaagtgaag ctgggcttgc
     1801 tgcccggcgc gggcggaacg cagcgcctgc cacgcgtgct gggcgccgag accgcgttga
     1861 acatgatcgt gagcggcgag agcgtcaaga gcgaattgct ggcgcaacag cccggccaga
     1921 agctgttcga ccgcatggcc gcttcacccg agtcgctgat ggaggaggcg atctctttcg
     1981 cccgctccgt ggccgatacc cgtccgctgc cgctggtgcg caacctgcct tgcaagcatc
     2041 cgctgggcga cgcctatttc cagttcgcgc gcaacatggt caagggcatg gccaagaact
     2101 tcccggcgcc cgccaagtgc gtcgacgccg tcgaggcggc cacgaagaag aagttcgatg
     2161 acgggctggc gctggaacgc gagatcttcc tgaacctgat gctcacgccc gaaagccgcg
     2221 cgctgcgcca tgtcttcttc gccgagcgcg cgacctccaa gatcccggac gtgccggccg
     2281 agacgccgca gcgcgagatc aagtcggtgg ccgtgatcgg cgccggcacc atgggcggcg
     2341 gcatctcgat gaacttcctg aatgccggga ttcccgtgaa gatcctggag atgaagcagg
     2401 aagcgctgga ccgcggcatc gccaccatcc gcaagaacta cgagtcgcag gtcaagaagg
     2461 gcaagctgaa ggtggacaag tacgaacagc gcatgagcct gctgtccacc accctcgatt
     2521 acaacgacct gaaggacgcc gacctggtga tcgaggcagt gttcgaggag atgggggtca
     2581 agcagaaggt gttcgagaag ctcgacgagg tgggcaagcc cggcgcgatc cttgcctcca
     2641 atacctcgac cctggacgtg aacaagatcg cctcgttcac caagcggccg caggacgtgg
     2701 tcgggctgca cttcttcagc ccggccaacg tgatgaagct gctggaagtg gtgcgcggcg
     2761 ccaagaccgg caaggacgtg atggccacgg tgatgaccct cgccaagaag atcaagaaaa
     2821 ctgcggtggt ctccggcgta tgcgacggct tcatcggcaa ccgcatggtc gagcagtaca
     2881 gccgccaggc cggtttcctg ttggacgagg gagccacgcc acagcaggtg gacaaggcga
     2941 tcgagaagtt cggcttcgcc atgggtccgt tccgcatggg cgacttggcc ggcaacgaca
     3001 tcggctgggc catccgcaag cgtcgtgcgc aggagcgccc gaacatgctc tacagccgca
     3061 ccgccgacaa gctgtgcgaa ctcggccgct tcggccagaa gaccggcgcg ggctggtacg
     3121 actaccaggc cggcaagcgc gacgccatcc cgtccgagct ggtcaacaag atgatcgagg
     3181 accaccgcaa ggagctgggc atcacgccgc gcaagatctc cgacgaggaa atcgtgcagc
     3241 ggctggtgtt cgccctggtc aacgaaggcg cgcgcatcct ggaagacggc atcgcttcgc
     3301 gggccagcga catcgacatg gtctacctca ccggctacgg cttcccgctg catcgcggcg
     3361 gcccgatgct ctatgccgac caggtgggcc tgttcaatgt gatgcaggcc atgaaacgct
     3421 tccagcgcaa cccgcacgat gacgcgaagt tctgggaacc cgcgccgctg ctggcgcgcc
     3481 tggcggccga aggcaagaac ttcaactgac gtccggaagg gcccgcgaag cggggccgcc
     3541 gtacgtcatc ccggcgcagg ccgggatcca tgactttcag cgcaacgatt tgatttatta
     3601 gagagaaccg ccatgagcaa cgcagtcatc gtctccaccg cccgcacgcc cctggccaag
     3661 agctggaagg gcagtttcaa catgacgcac ggagccacgc tgggcgggca cgtggtcaaa
     3721 cacgcgctcc agcgcgccgg catcgatgcc gccgccgtgg aagacgtgat catgggctgc
     3781 gccaatcccg agggcgcgac cggtgccaac atcgcgcggc agatcgcgat catggccggc
     3841 tgtccggtga ccacggccgg catgaccgtc aaccggttct gttcgtcggg cctgcaaacc
     3901 atcgcgctgg cgtcgcaacg cgtgatcgcg ggcgagggcg aggtgttcgt cgccggcggc
     3961 gttgaaagca tttcctgcgt gcagcaggaa atgaacacgc acatgatgaa ggaccccgac
     4021 ctggacaagc tcaagcccga gatctactgg tcgatgctgc agaccgccga gcaggtggcc
     4081 aagcgctaca agatcgagcg cgatgtaatg gacgaatatg gtgcggtcag ccagcagaag
     4141 gcctgtgcgg cacaagccga ggggaggttc gacgccgaaa tcgcgccgat cacggtgaag
     4201 gccggcgtgg ccgacccggt gatggggctg cgcaccaagg aagtcaccgt cagcaaggac
     4261 gaaggcaccc gtgaaggcac gaccaaggaa ggcatcggcg gcatcaagcc cgcgctgccc
     4321 ggcggtgtga tcgcggcggg caatgccagc cagttctccg acggcgccgg cgcctgcgtg
     4381 gtggtgagcg aagagtacgc ggcgaggaac aacctgaagc ccctcggccg cttcctcggc
     4441 tttgccgtcg ccggctgcga gcccgacgag atgggcatag gcccggtgtt cgcggtgccc
     4501 aaggtgctga aaaagctggg cctgaaagtg agcgacatcg acctgtggga attgaatgaa
     4561 gcgttcgccg tgcaggtgat ctactgccgc gaccggctgg gcatccccaa cgaccgcctg
     4621 aacgtcaacg gcggagccat tgccgtgggc catccctatg gtgtttccgg ccagcggctg
     4681 accgggcatg ccctgatcga aggcaagcgc cgcggcgcca agcgcgtcct cgtgacgatg
     4741 tgcatcggcg gcggcatggg cgcggcgggg gtgttcgaag ttctgtaagg ctgtaatccc
     4801 ggacccgaac ccggatccac cggacgtcat cccgggcttg acccgggatc catgtctctg
     4861 tcggacgcgg ccgaagacat ggattgcggg gccgggccgg aacgtgatgc ctcggaacgt
     4921 gcatagcgta ggcccggcat tgggctcgct gcactggaca gctgcgcgcc cccgggcgtt
     4981 ccgcgagccc agcccgtcat gacagattat tggagcgcgt gtatgtctct gcggccgacc
     5041 ttgaatttcc ttctctacga ctggctcaac gccgaagagc tgacggcccg ctcgcgtttc
     5101 gccgatcact cgcgcgaaac gttcgatgcc gtcttcgata cctgcgagcg gatcgcgcgg
     5161 gagaaatatg cgccgttcaa ccgcacggtg gacaccgagg agccgcgctt cgacggcgaa
     5221 aaggtgatcc tgcccaaagc gacgcacgag gcccacaagg cgtttgccgg gtcgggcatg
     5281 ctgagcgcgg cgcaggacta cgaaatcggc ggcatgcagt tgccgtacac ggtcgaggcg
     5341 gcgtcgcaca cgtttttcgc catggcctcc gccagcatcg gctcgggcat gctcacggtg
     5401 ggcaacgcga acctgctgat ggcgcatgga accgagccgc agaaaacggt gtttgcgctg
     5461 aacgagtttt ccgggcgctg gtcgggcacg atgtgcctgt ccgaacccca ggcggggtcc
     5521 tccctttccg acatcacgac gcgggccgtg ccggacggag atggcttcga gggcgatccg
     5581 ctgggaccgc gctaccgcct gcgcggcaac aagatgtgga tttcggccgg tgagcacgag
     5641 ctgaccgaga acatcatcca cctggtgctc gccaagattc ccgggccgga cggcaagctc
     5701 attgccggca cgcggggcat ttcgcttttc atcgtgccga aaatgctggt gaacgagcgc
     5761 ggcgaactga cgggcgagcg caacgacgtc gcactcgccg gcctgaacca caagctcggc
     5821 tggcgcggca caaccaatac gctgctcaat ttcggggagg gcaagttccc ggtggacggg
     5881 caggcgggcg ccgtggggta tctggtcggc cggccgcacg aaggcctgcg ttgcatgttc
     5941 cacatgatga acgaggcgcg catcggcgtc gggatggcgg ccgtgatgct gggcatggcc
     6001 ggctatcacg catcgctcga ctatgcgaag aaccggccgc aaggccgtcc cgttggcccc
     6061 aggggcaagg acccggcgca gccgcaggtg cgcatcatcg agcacgccga tgtcaagcgc
     6121 atgctgctgg cgcagaagtc ctattgtgaa ggcgccctgg ccctggaact gtattgcgcg
     6181 cggctcgtgg atgagcagca cacgggcgag ccgcaggcgg ccgacgaggc gcggctgctg
     6241 ctggaggttc tcacgccgat tgccaagagc tggccgagcg agtggtgcct ggaagccaat
     6301 tcgctggcga tccaggtgca cggcggctat ggctacacgc gcgacttccc ggtggagcag
     6361 tactggcgcg acaaccgcct gaacatgatc catgagggca cgcacggcat ccaggcggcc
     6421 gacctgctcg gacgcaaggt gctgatggaa ggcggccgcg gccttcagct gctggccggg
     6481 cggatcggcg cgacgatcga gcgcgccagg cagcactccc gcttccccga agaggctcgc
     6541 gcgctggacc aggctctgcg cgacgtgcaa gcggcgacgc aagccgcctg gtcgacaggc
     6601 gatccggcac aggccctggc caacgccgtg ccctacatgc aggccttcgg gcatatggtg
     6661 ctggcctgga tctggctgga cgtggtgctc gcgacacctg aagcagcatc gggcgagccg
     6721 gcgcaccagg gacggttgcg ggcggcgcgg tatttttatc gctacgagct gccgaagatc
     6781 ggcgcctggc tgaacgtggc cgccacccgc gatgagacct gcgccgggat gccggaggag
     6841 gccttctgat ggcctccgcc ttgaccagac gggttgaggt ttgccatatc actgccggga
     6901 cagacaaacg cccgcggcga acgcacaatc ggtggccgca ccactgcaag gaaagaatac
     6961 atgacccgtt ccgtttcaca actcttcgac ctcaaaggca ggacggcgct ggtgaccggc
     7021 ggttcgcgcg gcctgggcct gcagatggct catgccctgg gcgaagccgg ggcccgcgtc
     7081 atgctgacct cgcgcaaggc agacgacctg gaacaggcag ccgccgaact gcagtccgcg
     7141 ggcatcgacg cgcgctggat cgcggccgat tgcggcaagg aatcggatat ccaccgactg
     7201 gccgatgaaa cgctgcagcg catgggtgac gtggacatcc tggtgaacaa cgcgggcgcg
     7261 gcgtggggcg caccggcaga ggaccatccg gtcgaagcct gggacaagct gatgaacctg
     7321 aacgtgcggg gctacttcat cctgtcgcag cgcattgcca agcgcagcat gattccgcgc
     7381 aagtacgggc gcatcgtcaa catcgcgtcg atcgcgggcc tgggcggcaa tccgccggag
     7441 atgcagacgc tggcctacaa cacttccaag ggggcggtga tcaacttcac gcgggcgctg
     7501 ggttgcgagt ggggcaagta caacatcacc gtgaacgcca tctgcccggg tttcttcccg
     7561 agcaagatga cgaaggcgac gttcgagcgg ctgggcgagg cggaacttgc cgcccatgca
     7621 ccgctgcagc ggctcggcga cgacgaggac ctgaagggtg tgacgctgct cttcgcctcc
     7681 gaggccggca agcacatcac gggccaatgg ctggcggtgg acggcggcgt gagcgtggtc
     7741 accggcggct aggggggcct tgacatgcaa ttcggcgtcg acatcccttt cgtgcatcac
     7801 ctcgggttcg aactcgtgca attcgagggc ggccgctccg agatccagta cgaggccagg
     7861 ccggagcacc tcaattcctt cgcggtcacc catgggggcg cgtgcatgac cctgctggac
     7921 gtcacgatgg cggctgcggc tcgcagcgtg gacaaaaaca tgggggtagt caccatcgag
     7981 atgaagacca gcttcatgca ggcagcgcgc ggcaagctgg ccggccgcgg ccgcctgatg
     8041 caccgcacgg cgacgatggc tttcaccgaa gccaccattt acgacggtga aggccgcgct
     8101 tgcgcccacg ccaccggcac tttcaagtac gtcaaacgcc tgcccacggg cgcaaagagc
     8161 gtcctcccat tgaacatcat ttccacagac tgagaccttg cgggacagat caagaattgc
     8221 gcagcaattt tgctctgtcc tcaactggct aagcaatttt gctctgtcct caactgatta
     8281 gagagcccta tgccccaaaa caagcaattc ctcctcgaca accgccccca gggcgaggcc
     8341 accgccggca acttcaagct ggtcactggc gaaacaccgc ccctgcagga tggccaggtg
     8401 ctggtgcgcc accatttcct cagccttgac ccgtacatgc gcggccgcat gaacgatgcc
     8461 aagagctatg ccgcacccca gccgctgggc caggtgatgc aaggcggtac ggcgggcgaa
     8521 gtggtggaaa gccgctcgcc caaatatcag ccgggcgaca aggtggtcgg cagcggcggc
     8581 tggcaagagt acagcgtggt cgatgccgac cagcccggtg cgctgcgcaa ggtcgacacg
     8641 acgcaagtgc cgctgtcgca ttacctgggc gcggtgggaa tg
//
LOCUS       DASWIY010000299         5361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3511214, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000299 DASWIY010000000
VERSION     DASWIY010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3511214"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..337)
                     /gene="ffs"
                     /locus_tag="VGA59_11020"
     ncRNA           complement(241..337)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGA59_11020"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            415..2277
                     /gene="dnaX"
                     /locus_tag="VGA59_11025"
     CDS             415..2277
                     /gene="dnaX"
                     /locus_tag="VGA59_11025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX9720253.1"
                     /translation="MSYLVLARKYRPKTFGEMVGQEHVVQALSNALTQQRLHHAYLFT
                     GTRGVGKTTVSRILAKSLNCQGPDGQGGITATPCGVCQACSDIDSGRFPDYTELDAAS
                     NRGVDDMQSLLEQAVYKPVQGRFKVFMIDEVHMLTGHAFNTMLKTLEEPPEYLKFVLA
                     TTDPQKVPVTVLSRCLQFNLRPMAPETMQEHLVRVLDAEGVPSEAPALRLLARAARGS
                     MRDALSLTDQAIAFGGGKLEEATVRQMLGSVDRGHVFRLIDALAQADGRTVVEISEAL
                     RTLGLSAASTLEEMSAVLQRMAVIQAVPAAADNSDSETAEIARLAGLMPADETQLLYS
                     LCLHGRAELGLAPDEYAGLTMVLLRLLAFKAPAVREPQKKTLNNGDTGLAPQRSPASP
                     VVARPTPAPAAPLQAPAPARTAVPATGAPPGRVLRVVEAPPPQPAQRGASGDAPNGRP
                     AGEVVGVPVRVQSESMREATPATTALVPTEEGDFWHATVTGLVAREAITALVRELALQ
                     SQLVGRDEGHWMLRVERESLNQPTSRERLQNALATTGHRVSLSVEVGAVTDSPARRMA
                     AASAARQRAAEEIIHGDPFVQAMMREFGAKIVPGSIKPVTNDRGDRPSALSQTD"
     gene            2292..2615
                     /locus_tag="VGA59_11030"
     CDS             2292..2615
                     /locus_tag="VGA59_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX9720254.1"
                     /translation="MFNKGQLAGLMKQAQAMQDNIKKAQEELATIEVSGESGAGLVKV
                     TMTCKHDVKRVTIDPSLLAEDKDMLEDLVAAAFNAAVRKAEETSQEKLGKITAGLPPG
                     MKMPF"
     gene            2615..3205
                     /gene="recR"
                     /locus_tag="VGA59_11035"
     CDS             2615..3205
                     /gene="recR"
                     /locus_tag="VGA59_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX9720255.1"
                     /translation="MSDTNALTALIEALRRLPGVGVKTASRMAFHLLQHDRAGAQVLA
                     QALQQAASQIRHCSLCHTFTENDVCATCLDPRRDRSKLCVVETPADQAAVERTAAFRG
                     LYFVLMGKLSPLDGIGPKDIGLAKLFARATDGEVQEVILATNFTAEGEATAHVIGQAL
                     KERGLNVTRLARGVPAGSELEYVDLGTIAHALVDRR"
     gene            3336..4382
                     /locus_tag="VGA59_11040"
     CDS             3336..4382
                     /locus_tag="VGA59_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9720256.1"
                     /translation="MRNIPFTRRTFTSVAALAVASIAAPVLRAQSKLEKSKVAIAVGG
                     KAAFYYLPLTVAEQLGYFKAEGLDVEISDFAGGARALQALIGGSADVVSGAYEHTINM
                     QSKNLNIQAFVLQGRAPQIALGVSTKAMPNYKSLADLRGKKIGVSAPGSSTNMVANLV
                     LSRAGIKASDVSYVGVGTSAGALTALRSGQIDAMSNTDPVMTMLEQKGDVKIISDTRT
                     LKGTIDVFGGPMPAACFYARAEFIQKNPNTCQALANAIVHGLKWLQTAGPSDIVKTVP
                     EGYLLGDRALYLASFNKVREAIALDGLIPEEGARTALKALASFDPAIKADKIELSRTY
                     TNEFARRAKEKFKA"
     gene            complement(4538..5107)
                     /locus_tag="VGA59_11045"
     CDS             complement(4538..5107)
                     /locus_tag="VGA59_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720257.1"
                     /translation="MFKKTLLGVIVAAGSLVSLLPTLANAQYSAVISVAPPAPMHEAV
                     PPPRRGYVWAPGHYEWRNSRYVWIAGHWLQARSGHEYREPRWVQRGNGEWYLVGGNWE
                     RRGPYGDRDRDGIANRYDRDKDGDGIPNATDDRPNRADYGRNDRRDRRFGPYGDLDRD
                     GIRNRDDRDRDGDGIRNRRDRFPDNPRRS"
BASE COUNT          964 a         1763 c         1798 g          836 t
ORIGIN      
        1 gcctgaatcg ctccggcgcc accgccccgg cccgcttgat cttccgccac gccgtcatcc
       61 gttcgcccgg catatagatg gctggaaccg cgcgagaaaa ggccggtcag tttgctgatc
      121 ggatgctcca cgtttgttga acgtgacacc cgaagtgaca caggaagatg acacaggatg
      181 gtccgatttg ctcgaccagc gactgcaagt cgttgatctt gaaggacatc cttgacgaag
      241 gttgacgagc cttacccccg gcactggctc cacagttagg gctgcttggt tccccacctg
      301 acccggttgg ccgcttcacc atgcggggag gcccgtcagc cgtcattgta ggccgagggc
      361 aaagcccctc gctgtcgcgg ggaccgccat gaggcagccc gtagaatccg gcgcatgtcc
      421 tatctcgtgc tcgcccgcaa gtaccggccc aagaccttcg gcgagatggt cggccaggaa
      481 cacgttgtgc aggcgctcag caatgcgctc actcagcagc ggttgcacca tgcctatctt
      541 ttcaccggaa cgcgcggagt cggcaagacc acggtgtcgc gcatcctggc caagtccctc
      601 aactgccagg gcccggacgg gcagggcggc atcacagcga cgccgtgcgg cgtgtgccag
      661 gcctgcagcg atatcgacag cggccgcttt ccggactaca cggaactgga tgccgcttcc
      721 aatcgcggcg tggacgacat gcagtcgctg ctggagcagg cggtgtacaa gccggtgcag
      781 ggccgcttca aggtcttcat gatcgacgaa gtgcacatgc tcacgggcca cgccttcaac
      841 accatgctca agacgctgga agagccgccc gagtacctca agttcgtgct ggccaccacc
      901 gatccgcaaa aggtgccggt gacagtgctg tcgcgctgcc tgcagttcaa cttgaggccg
      961 atggcgccgg agacgatgca ggagcatctg gtgcgggtgc tggacgccga gggcgtgccg
     1021 tcggaggccc cggccttgcg cctgctcgcg cgcgccgcgc gcggctccat gcgcgatgcg
     1081 ttgtcgctga ccgaccaggc gattgctttc ggcggcggca agctcgagga agcgaccgtg
     1141 cgccagatgc tgggcagcgt ggaccgcggc cacgtgttcc ggttgatcga cgcgctggcg
     1201 caagccgacg gcaggaccgt ggtcgaaatc tcggaggcct tgcggactct gggcctgagc
     1261 gcggcctcga ctctggagga gatgagcgcg gtgctgcagc gcatggccgt gatccaggcc
     1321 gtgcccgcag cggccgacaa cagcgacagc gaaaccgccg agatcgcgcg gctggccggg
     1381 ctgatgccgg cggacgaaac ccagctgctc tacagcctgt gtctgcacgg ccgcgcggag
     1441 ctgggcctgg cgccggacga gtacgcgggg ctgaccatgg tgctgctgcg cttgctggct
     1501 ttcaaggcgc cggcggttcg cgagccgcaa aaaaaaactc tgaataatgg cgataccggg
     1561 cttgccccgc aacgcagtcc ggcgtcaccg gtagttgccc ggccgacacc ggctcctgcc
     1621 gcgcccttgc aggctccggc tccggcgcgc acggcggtcc cggcgacagg tgccccgccg
     1681 gggcgggtgc ttcgggtagt ggaagcgccc cccccacagc cagcccagcg gggcgcgagc
     1741 ggcgacgcgc cgaacggccg gcccgccggt gaggtcgtcg gggtgccggt gcgcgtgcag
     1801 tccgagtcca tgcgcgaggc gacgcccgcg acgaccgccc tggtgccgac cgaagagggg
     1861 gatttctggc atgcgaccgt gaccggcctg gtggcccgcg aggcgatcac ggcgctggtg
     1921 cgtgagctgg cgctgcaatc gcagctggtc gggcgggacg agggccactg gatgctgcgc
     1981 gtggagcgcg agtccctgaa ccagcccacc agccgggagc ggctgcaaaa tgcgctggca
     2041 acgaccggcc accgggtgag cctgagcgtg gaggtgggcg cggtcaccga cagtccggcc
     2101 cggcgcatgg cggccgcctc ggcggccaga cagcgggcgg ccgaggaaat catccacggc
     2161 gatcccttcg tccaggcgat gatgcgtgag ttcggcgcga aaatcgtgcc tggcagcatc
     2221 aagcccgtaa ccaacgatcg cggggacaga ccttcagcgc tgtcccaaac tgactaagta
     2281 aaggactgat catgttcaac aagggacaac tcgccggcct catgaaacag gcgcaggcca
     2341 tgcaggacaa catcaagaag gcgcaggagg agctggccac catcgaagtc tccggcgaat
     2401 ccggcgccgg gctggtgaag gtgacgatga cgtgcaagca cgacgtcaag cgcgtgacca
     2461 tcgatcccag cttgctggcc gaggacaagg acatgctcga agacctggtg gccgccgcct
     2521 tcaatgccgc tgttcgcaag gccgaggaaa cctcccagga gaagctgggc aagatcaccg
     2581 ccggcctgcc gcccgggatg aagatgccgt tttagtgtcc gacaccaacg ccctcactgc
     2641 cctgatcgag gcgctgcgcc ggctgccggg cgtcggcgtc aagactgctt cgcgcatggc
     2701 attccacctg ctgcagcatg accgggcggg cgcccaggtg ctggcacagg ccctgcagca
     2761 ggccgcctcg cagatcaggc attgcagcct ttgccacact ttcaccgaaa acgacgtctg
     2821 cgccacgtgc ctggacccgc ggcgcgaccg cagcaagctt tgcgtggtgg aaactcccgc
     2881 cgatcaggcc gcggtggagc gtacggccgc cttcagggga ctttatttcg tgctgatggg
     2941 caagctcagc ccgctggacg gcatcggtcc caaggacatc ggcctggcga agctgttcgc
     3001 gcgcgccacg gacggcgagg tgcaggaggt gatcctggcg accaacttca ccgcggaggg
     3061 tgaagccaca gcgcatgtca tcgggcaagc cctcaaggag cgcgggctga acgtcacacg
     3121 gctggcgcgg ggcgtgccgg cgggcagcga actggagtac gtcgacctgg gcacgatcgc
     3181 ccacgcgctc gtggaccgga gataactggg aatgaggtcc tgttgcgcgg cccgccgcgc
     3241 agcgacaact tacgtggaaa cccgctcggg atgttcccga tgcggtgtgc cggccccccg
     3301 actaccctcg aaaacacgca gagaggaccc atccaatgag gaatattccg ttcacgcgac
     3361 gaacgttcac ttcggtcgcg gcactcgccg tcgcctcgat cgccgcgccc gtgctgcgcg
     3421 cgcagagcaa gctcgagaaa agcaaagttg cgatcgccgt gggcggcaag gccgccttct
     3481 actaccttcc cctcactgtc gccgagcagc tgggctactt caaggccgaa ggcctggacg
     3541 tcgagatctc ggatttcgcc ggtggcgcgc gggccctgca ggcactgatc ggcggctccg
     3601 ccgacgtggt gagcggcgcc tacgagcaca ccatcaacat gcagagcaag aacctgaaca
     3661 tccaggcgtt cgtgctgcag ggaagggcgc cgcagatcgc cctgggcgtt tcgaccaagg
     3721 ccatgcccaa ctacaagtcg ctggccgacc tgaggggcaa gaagatcggc gtctccgccc
     3781 ccggctcttc caccaacatg gttgccaacc tggtactctc gcgcgccggc atcaaggcca
     3841 gcgacgtgag ctatgtggga gtgggcacat ccgccggcgc gctgaccgcg ttgcgctcgg
     3901 ggcaaatcga cgccatgagc aacaccgacc cggtgatgac gatgctggag cagaagggcg
     3961 acgtgaagat catcagcgat acgcgcacgc tcaagggcac catcgacgtc ttcgggggac
     4021 ccatgcccgc agcctgcttc tacgcgcgcg ccgagttcat ccagaagaac ccgaatacct
     4081 gccaggcgct ggccaatgcc atcgtgcacg gcctgaaatg gctgcagacg gcgggcccca
     4141 gcgacatcgt caagacagtg cccgaaggct accttctggg cgaccgcgcg ctctacctgg
     4201 cttccttcaa caaggtgcgc gaggcgatcg cgctcgatgg cctcatcccg gaggaaggcg
     4261 cccgcacggc actgaaggcg ctggccagtt tcgatcccgc catcaaggcc gacaagatcg
     4321 agctgtccag gacctatacc aacgagttcg cgcggcgggc gaaggaaaag ttcaaggcat
     4381 aagcttcaac cgccgcgccg gctacatgtt ccagaccgag gcatcgaagc ggctcatggt
     4441 gtggcagccg cgccggggcg agaccgaaaa gctctgacct gtcgcgtcaa gccggaaaaa
     4501 aagcccggtg ccatggccgg gcttttttga tcaggcgtta gctgcgccgc gggttgtccg
     4561 ggaagcggtc gcgccggttg cgaatgccgt caccgtcgcg gtcgcggtca tccctgttgc
     4621 ggatgccgtc gcggtcgagg tcgccgtagg ggccgaagcg ccggtcgcgg cggtcgttac
     4681 ggccatagtc ggcgcggttg ggccggtcgt cagtggcgtt ggggatgccg tcgccgtctt
     4741 tgtctctgtc atatcggttg gcgattccat cgcggtcgcg gtcgccgtag ggcccgcgcc
     4801 gttcccagtt gccgcccacc aaataccact cgccattgcc gcgctgcacc cagcgcggtt
     4861 cgcggtattc gtgaccggag cgagcttgca gccaatgccc ggcaatccac acataccggc
     4921 tgttgcgcca ctcgtagtgg cccggcgccc acacataacc ccggcggggc ggcggaacag
     4981 cttcatgcat cggcgccggc ggagcaaccg aaatgacggc tgagtactgg gcgttggcaa
     5041 gggtcggcag caggctcacc agcgagccgg ctgccacgat gacacccaag agcgttttct
     5101 tgaacatggt cgaactccaa tctagagaca gtaagaccac cttgaaccgg gcgcgcccgc
     5161 cccggcatcg gaatcgggag caattactcg tcggagccgt cctgcgcggc cgatgtgaca
     5221 agttgtagcc ctaccgccgg gccggcgtga aatcagcgca ccggcttgga tcgccgcttg
     5281 gtgacagcgc gcttggcggg cctggcgccg tgtttgccgg ccttggcacg aattcccggc
     5341 ttgacgccgg tgcgtgcccg g
//
LOCUS       DASWIY010000300         4977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1302114, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000300 DASWIY010000000
VERSION     DASWIY010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302114"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="VGA59_11050"
     CDS             complement(<1..1342)
                     /locus_tag="VGA59_11050"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX9720258.1"
                     /translation="MDIKTIKINRRSSNITEGKSRAPNRSMYYAMGYGEGDFSKPMVG
                     VANGHSTITPCNSGLQKLADAAVAGIEEAGGNAQIFGTPTVSDGMAMGTEGMKYSLVS
                     REVISDCVETCVQGQWMDGVLVVGGCDKNMPGGLMGMLRANVPAIYVYGGTILPGHWK
                     GQDLNIVSVFEAVGQNSAGNLSDADLREIEMRAIPGTGSCGGMYTANTMSSAFEALGI
                     SLPYSSTMANPHDEKTNSARESAKVLIEAIRRDIKPRDIVTRKAIENAVAVVMAIGGS
                     TNAVLHFLAIAHAAGVEWTIDDFERVRKKTPVLCDLKPSGRYLAVDLHQAGGIPQVMK
                     ILLKAGLLHGDCLTITGRTVAEVLEDVPDEPREDQDVIRPIDKPMYEQGHLAILKGNL
                     SPEGCVAKITGLKNPVMTGPARVFDDEQSALQAILDGRIKAGDVMVLRYLGPKGG"
     gene            1495..2415
                     /locus_tag="VGA59_11055"
     CDS             1495..2415
                     /locus_tag="VGA59_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9720259.1"
                     /translation="MSQHLVELRLWRQFIAVAEELHFGRAALRLHMTQPPLTQAIAGL
                     ERLLGVRLFDRTKRSVHLTAAGHALLPEARELLARALALPAHARAAAHGESGRLRLAF
                     VSTVGFGLLPQWVLAFRSIYPQVELELIESTGDVQMQALERGDIDAGFMLHSPGFAPA
                     GLSHLRILREPLVLALPEQHELAVAHTLPLGRVLDQPLVLFPRRILPSIHDAVFGMYH
                     AAGRTPRIAQEAIQMQTIVNLVSAGLGLAWVPDSVRQFQRPGVVYRQIGARKAQAVPA
                     CETTLVWAPTANPTLERFVEFARGRLAAPG"
     gene            2480..3310
                     /gene="lgt"
                     /locus_tag="VGA59_11060"
     CDS             2480..3310
                     /gene="lgt"
                     /locus_tag="VGA59_11060"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX9720260.1"
                     /translation="MLMYPRIDPVALQLGPVAIHWYGLTYLAAFGLFLLLALRRLQHE
                     PFASVRGQGAWSRKDVEDMLFFGVLGVVIGGRLGYCLFYKPGYYLAHPLEIFFVWQGG
                     MSFHGGMLGVIVSQWLFARSRGKPFWQLMDFVAPCVPLGLAAGRVGNFINGELWGRAA
                     DPSLPWAMVFPQSGSMIPRHPSQVYQFLLEGLLLFVILWLYGRKERKPAQVSAVFLMG
                     YGLLRFIAEYFREPDAHLGLLSLGLSMGQWLCIPMVLAGLLLWLWGGSRPAPLPAKAR
                     "
     gene            3324..4787
                     /locus_tag="VGA59_11065"
     CDS             3324..4787
                     /locus_tag="VGA59_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HEX9720261.1"
                     /translation="MNSSDWIARSVSLLRPGLEPRAVATQSGAETAPRAAGRPAPAQR
                     STHERLQATLRRHQEALSPRVLRRTLAELKAIIDPQVSEVEAGRRARGVAGWYAKATP
                     EQRRDCWLLMSEQFAPDPKRIQAARTQYEAVQGTGEEGQAEIRLRKAFVSPRTRLLQR
                     FAAFPEGLRFLVDMRAELLQQLRSDKRLLALDAELENLFSTWFDVAFLELRRISWDSP
                     ASLIEKLIKYEAVHDIRSWADVKNRLDSDRRCYGFFHPRLDSEPLIFVEVALMDQMAG
                     GITPLLDEAAAPSDLVRATTAIFYSISNTQNGLRGVSFGDSLIKRVVETLKGEFPRLK
                     VFATLSPIPGLRLWLAKNAAAMIERLDPKPRAELGRALGAEPTAELLLAAFEKPLELD
                     PKSPVRLMLLGCAAQYLGRESQDGKPLDAVARFHLGNGARIERLNWAGDPSAKGLKQS
                     YGLMVNYLYDLKRLDKHRVRLSQGQIPISGAIEDLYF"
BASE COUNT          739 a         1645 c         1651 g          942 t
ORIGIN      
        1 gtccgccctt gggtcccagg tagcgcagca ccatcacgtc gccggccttg atcctgccgt
       61 ccaggatcgc ctgcagggcc gactgttcgt cgtcgaacac ccgggccggc ccggtcatga
      121 ccggattctt caggccggtg atcttggcga cgcagccttc gggggagaga ttgcccttga
      181 ggatcgccag gtgcccctgc tcgtacatcg gcttgtcgat cggccggatc acgtcctggt
      241 cctcgcgcgg ttcatcgggc acgtcctcga gtacctcggc gacggtccgg cccgtgatgg
      301 tgaggcagtc tccgtgcaac agtccggctt tcagcaggat cttcatcacc tgcggtatgc
      361 cgcccgcctg gtgcaggtcc accgccaggt agcggccgct gggcttcaag tcgcacagga
      421 ccggcgtctt cttgcggacg cgctcgaaat cgtcgatggt ccactccacg cccgccgcgt
      481 gcgcgatcgc caggaagtgc agcaccgcgt tggtcgagcc gccgatcgcc atgacgacgg
      541 ccacggcgtt ctcgatcgcc ttcctcgtga cgatgtcgcg tggcttgatg tccctcctga
      601 tcgcctcgat cagcaccttg gcggattccc tggccgagtt cgtcttctcg tcgtgcgggt
      661 tggccatggt ggacgagtaa ggcagcgaga tgcccagcgc ctcgaacgcc gacgacatgg
      721 tgttggcggt gtacatgccg ccgcacgatc ccgtgccggg gatcgcccgc atctcgatct
      781 cgcgcaggtc ggcgtcgctc aggttgccgg ctgagttctg gcccaccgcc tcgaacacgc
      841 tgacgatgtt caggtcctgg cctttccagt ggcccggcag gatcgtgcca ccatacacgt
      901 agatggcggg cacgttggcg cgcagcatgc ccatcaggcc gccgggcatg ttcttgtcgc
      961 agccgcccac caccagcacg ccatccatcc actggccctg cacgcaggtc tcgacgcaat
     1021 ccgagatcac ctcgcggctg accagcgagt acttcatgcc ttccgtgccc atggccatgc
     1081 cgtccgagac cgtcggcgtg ccgaagatct gcgcgtttcc gccggcttcc tcgatgcccg
     1141 ccacggccgc gtcggcgagc ttttgcaggc cgctgttgca gggcgtaatc gtgctgtggc
     1201 cgttggccac gccgaccatg ggcttgctga agtcgccctc cccatagccc atcgcgtagt
     1261 acatcgagcg gttcggcgcg cgcgacttgc cttctgtgat gttgctgctg cggcggttga
     1321 tcttgatcgt cttgatgtcc atgtgtcctc catgacccgt tcgggccggg cttgccggga
     1381 tggggcaggc tcggggcgaa cggagggttg tccggcctgg gaaggccacg agtatgcgcc
     1441 gggccattta ttctttccaa tccgttttcg atgctgtatt aatatgctgg gcgtatgagt
     1501 caacacctgg tcgagctgcg cctgtggcgc cagttcatcg ccgtcgccga ggaactgcac
     1561 ttcggccgcg ccgcgttgcg cctgcacatg acgcagccgc cgctcaccca ggccatcgcc
     1621 ggcctcgagc ggctgttggg cgttcgcctt ttcgatcgca ccaaacgcag cgtgcatctg
     1681 acggccgccg gacatgcgct attgcccgaa gcgcgcgagc tactggcgcg ggcgctggcc
     1741 ctgccggcac atgcccgcgc cgccgcgcac ggcgagagcg gccgcttgcg gctggcgttc
     1801 gtttccaccg tcgggttcgg gctgctgcct caatgggttc tggccttccg ttcgatttat
     1861 ccgcaggtcg aactcgagct gatcgagtcc accggcgacg tccagatgca ggcgctggag
     1921 cgcggcgaca tcgatgccgg attcatgctg cattcgccgg gcttcgcgcc ggccggactg
     1981 agccacctgc gcattcttcg cgagccgctg gtgctggcgc tgcccgagca gcatgagctg
     2041 gcggtcgcgc atacgctccc attgggacgc gtgctcgacc agccgctggt gctatttccc
     2101 aggcgcatcc tgccgtcgat ccacgatgcg gtattcggca tgtaccacgc agctgggcgc
     2161 acgccgcgca ttgcgcagga ggcgatccag atgcaaacca tcgtcaacct ggtttctgct
     2221 gggctgggcc tggcctgggt gcccgatagc gtgcgccagt ttcagcggcc gggcgtggtt
     2281 tatcggcaga tcggcgcgag gaaagcgcag gccgtcccgg cgtgcgaaac gacgctggtg
     2341 tgggccccga cagcgaatcc gacgctggaa cgattcgtgg agtttgcgcg cgggcgtttg
     2401 gcggcgcccg gctgacgatt cctgcaggcc ggccgggccc ggtccccgca cccgcgggcg
     2461 gttgcagcac aatacccgca tgctgatgta ccccagaatc gaccccgtgg ccctgcagct
     2521 gggccctgtc gccatccact ggtatggact gacctacctt gccgcgttcg gcttgttcct
     2581 cctgctcgcg ctgcggcggc tgcaacacga gccttttgcg tcagtcaggg ggcagggggc
     2641 gtggagccgc aaggatgtcg aggacatgct ctttttcggc gtgctcggcg tggtgatcgg
     2701 gggacggctg ggctactgcc tgttctacaa gcccggctac tacctcgcgc atccgctcga
     2761 aatcttcttc gtgtggcaag gcgggatgag cttccacggc ggcatgctcg gagtcatcgt
     2821 ttcccaatgg ctgttcgcgc gctcgcgcgg caagccgttc tggcagttga tggatttcgt
     2881 cgcgccatgc gtgccgctcg gcctggctgc gggacgcgtg ggcaacttca tcaacggtga
     2941 actctggggc cgcgccgcag atccgtcgct gccgtgggcg atggtcttcc cacaaagcgg
     3001 ctcgatgatt ccgcggcatc cgtcgcaggt gtaccagttc ctgctggaag gcttgctgct
     3061 cttcgtgatc ctgtggctgt atgggcgcaa ggaacgcaag ccggcgcagg tctccgccgt
     3121 attcctgatg ggctacggct tgctgcgttt cattgccgag tactttcgcg agccggacgc
     3181 ccatctgggg ctgctctcgc ttggtttgag catgggccag tggctgtgca tccccatggt
     3241 gctggcgggc ctgctgctct ggctttgggg cggcagccgc cccgcgccac tgccagccaa
     3301 agccaggtag cgaggttggc gtcatgaact cgagcgactg gatcgcccgc agcgtgtcct
     3361 tgctgcgccc cggcctggag cctcgggcgg tggcgacgca gtcgggggcc gaaacggcgc
     3421 cgcgcgcggc ggggcgtccc gctcccgcgc agcgctccac gcacgagcgc ctgcaggcga
     3481 cgctgcgccg ccatcaggag gcgctgtcgc cgcgtgtgct gcgccgcacg cttgccgagc
     3541 tgaaagccat catcgatccg caggtcagcg aggtggaagc cggccgccgc gcccgcggcg
     3601 tcgccggctg gtatgccaag gcgacgcccg aacagcggcg cgactgctgg ctgctgatga
     3661 gcgaacaatt cgcgccggac ccgaaaagga tccaggcggc ccggacgcag tacgaagcgg
     3721 tgcaaggcac cggcgaggaa gggcaggccg aaatccgcct gcgcaaggct ttcgtgtcgc
     3781 cgcgcacgcg gctgctgcag cgcttcgccg cttttccgga aggcttgcgg ttcctggtgg
     3841 acatgcgtgc ggagctcctg cagcaactca ggagcgacaa gcgcctgctg gcactggacg
     3901 cggaactcga aaacctgttt tccacctggt tcgacgtggc cttcctcgag ctgcggcgca
     3961 tcagctggga ttcgccggct tcgctgatcg agaagctgat caagtacgaa gccgtgcacg
     4021 acatccgcag ctgggccgat gtgaagaacc ggctggacag cgaccggcgc tgctacggct
     4081 ttttccatcc gcggctggac agcgaaccgc tgatcttcgt tgaggtggcg ctcatggacc
     4141 agatggccgg cggcatcacg ccgctgctgg acgaggcggc ggcgcccagc gacctggtcc
     4201 gggcgaccac ggcgattttc tattccatca gcaacacgca gaacggcctg cgcggcgtga
     4261 gtttcggcga ttcgctgatc aagcgggtgg tggagacgct caagggcgag tttcccaggc
     4321 tcaaggtctt cgccaccctg tcgccgatcc cgggcttacg cctctggctc gcgaagaacg
     4381 cggccgcgat gatcgagcgg ctcgatccca agccgcgggc ggaacttggc cgtgcactgg
     4441 gcgcggaacc gacggccgaa ctcctgctcg ccgccttcga gaagccgctg gaactggacc
     4501 ccaagtcccc ggtgcgcctg atgctgctcg gctgcgcggc gcaatatctt ggccgggaat
     4561 cgcaggatgg caagccgctc gacgcggtcg cgcgattcca cctgggcaac ggggcgcgca
     4621 tcgagcggtt gaactgggcg ggcgatccct cggcaaaagg cttgaagcaa tcttatggat
     4681 taatggtcaa ctacctttat gacctgaagc gcctcgacaa acaccgcgtc aggctctcgc
     4741 aaggacaaat cccgatttct ggggcgatcg aagacctgta cttttgagca ttcggccgcg
     4801 gcaggccgat tgccggctgt gttgcccgct cgatgtgcgg cggtttcttg aaattcattc
     4861 ataacaagga gacaagggcc atggaccaca gccctgccag gcgagacgtt ttgcgcgccg
     4921 cctcggccgg cgccgcctcg ctggcggcgc ggttcgccaa gcagaccggc aagcagc
//
LOCUS       DASWIY010000301         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_937116, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000301 DASWIY010000000
VERSION     DASWIY010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937116"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1393)
                     /gene="ftsA"
                     /locus_tag="VGA59_11070"
     CDS             complement(164..1393)
                     /gene="ftsA"
                     /locus_tag="VGA59_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX9720262.1"
                     /translation="MAKEYKDLVVGLDIGTAKVMAVVAEVLQGGELKLAGLGVAASNG
                     LKRGVVVNIDATVQSIQQALKEAELMADCKITRVYTGITGSHIRGINSSGMVAVKDKE
                     VTAADVARVIETARAINISTDQRLLLVEPQEFVIDGQDVKEPIGMSGIRLEAKVHIVT
                     GAQSAAENIIKCVRRCGLEVEQLMLNALASSLAVLTDDEKELGVALVDIGAGTTDVAI
                     FTSGAIRHTAVIPIAGDLITSDIAMALRTPTKDAEDIKVESGYAKQLLADPEQQVEVP
                     GLGDRGPRMLSRQALAGVIEPRIEEIFSLVQQVVRESGYEEVLSSGIVITGGSAVMPG
                     MVELGEDIFLKPVRRGIPKYTGALSDMVAQARAATVMGLLEEARMARLRGFKVAQKNG
                     SMKTAFGRFKDFIVGNF"
     gene            complement(1459..2244)
                     /locus_tag="VGA59_11075"
     CDS             complement(1459..2244)
                     /locus_tag="VGA59_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX9720263.1"
                     /translation="MPATLQQPFDVKLMNITATALFSVCVMLLLAGVAWWAVRHPLFA
                     IAGITVQGDVAHNNAVTLRANVAPRLAGNFFTVDLGKAREAFEAVPWVRKAVVRREFP
                     NRLRVRLQEHQAVALWGADGETRLVNSFGEVFEANAGDVEPDELPRLSGPQEQSAQVL
                     AMHGSLRPMFEALDLGVVRLALTARGSWQLELDTGGVVELGRGTPEEVTARTQRFAQT
                     LTQVASRYGRRPDALISADLRHGDGFAVRLRGVGTTGADAAKR"
     gene            complement(2394..>2585)
                     /locus_tag="VGA59_11080"
     CDS             complement(2394..>2585)
                     /locus_tag="VGA59_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX9720264.1"
                     /translation="GWGRVDVMIDAATRKPWLLEINTSPGMTGHSLVPMSARAAGISY
                     EELCLQLLASAALDHEKGA"
BASE COUNT          376 a          940 c          823 g          446 t
ORIGIN      
        1 gtcggcggat cggtacatcg ccatcgctcc tttctcccgc ctttgggcga ggctcgcgca
       61 tgccaggcag ccatcgttcg ggggccaccg cggaaccggc tttgccgggc cgctggtggc
      121 gcccccctgg gggggaggcg gccgcaggcc gcttcggggg gggtcaaaag ttccccacga
      181 tgaaatcctt gaagcggccg aaagccgtct tcatcgaacc gttcttctgc gccaccttga
      241 agccacgcag ccgcgccatg cgcgcctctt cgagcaggcc catcacggtg gcggcgcgcg
      301 cctgcgcaac catgtccgat agcgcgcccg tgtacttggg aatgccccgg cggacgggct
      361 tcaggaagat gtcctccccc agctccacca tgccgggcat gacggcgctg ccgccggtga
      421 tgacgatgcc ggaggacagc acctcctcgt agccggactc gcgcacgacc tgctgcacaa
      481 gagaaaagat ctcctcgatc cgcggctcga tcacgcccgc cagtgcctgc ctggacagca
      541 tgcgggggcc gcggtcgccc agccccggca cctcgacctg ctgctccggg tcggccagca
      601 gctgcttggc atagccgctt tccaccttga tgtcctccgc atccttggtc ggggtgcgca
      661 gcgccatcgc gatgtcgctg gtgatcaggt cgcccgcgat cgggatcacg gccgtgtgcc
      721 ggatcgcgcc gctggtgaag atcgccacgt cggtggtgcc ggcgccgatg tcgaccagcg
      781 ccacgccgag ttccttctcg tcgtcggtca gcaccgccag gctggaggcg agcgcgttga
      841 gcatcagctg ctcgacttcc aggccgcagc ggcgcacgca cttgatgatg ttttcggccg
      901 cgctctgcgc gccggtgacg atgtgcacct tggcttccag ccggatgccg ctcatgccga
      961 tcggctcctt cacgtcctgg ccgtcgatca cgaattcctg gggctcgacc agcagcagcc
     1021 gctggtcggt ggagatattg atggcgcgcg cggtttcgat cacgcgcgcc acgtcggccg
     1081 ccgtgacttc cttgtccttc acggccacca tgccgctgga attgatgccg cggatatggc
     1141 tcccggtgat gccggtgtag acccgggtga tcttgcagtc ggccatcagc tcggcctcct
     1201 tcagggcctg ctggatgctc tggacggtcg cgtcgatgtt caccaccacg ccgcgcttga
     1261 gcccgttgga ggcggccacg ccgaggccgg ccagcttcag ctcgccgccc tggagcacct
     1321 cggccaccac cgccatcacc ttcgcggttc cgatgtccag ccccaccacc aggtccttgt
     1381 attcttttgc catttcttgc ctgtgttttg ccttctattc agttggcgac agagctaaag
     1441 atctgtcccc aaagtcattt atcttttcgc cgcgtccgca cccgtggtgc cgacgcccct
     1501 cagtcgcacc gcgaacccgt cgccgtgccg cagatcggcc gagatcaacg catctggccg
     1561 ccggccgtag cgcgacgcca cctgggtcag cgtctgcgcg aagcgctggg tgcgcgccgt
     1621 cacctcctcg ggcgtcccgc gccccaactc gaccacaccg ccggtatcga gctccagctg
     1681 ccagctgccg cgcgccgtga gggccagccg gaccacgccc aggtccaggg cttcgaacat
     1741 cggcctgagc gagccatgca tggccagcac ctgggccgac tgttcctgtg gcccggacag
     1801 cctgggcaac tcgtcgggct cgacgtcgcc cgcgttggcc tcgaacactt cgccgaagct
     1861 gttgaccagc cgggtctcgc cgtcggcgcc ccacaaggcc accgcctggt gttcctgcag
     1921 ccgcacccgc agccggttgg gaaattcgcg ccgcaccacc gcctttcgca cccacggcac
     1981 ggcctcgaag gcctcgcgcg ccttgcccag gtcgacggtg aagaaattgc cggccagccg
     2041 cggcgccacg ttggcccgca gcgtcaccgc gttgttgtgc gccacgtcgc cctgcaccgt
     2101 gatgcccgcg atcgcgaaca ggggatgccg caccgcccac caagccaccc cggccagcag
     2161 caacatgaca cacaccgaga acagcgccgt ggcggtgatg ttcatcagct tgacgtcgaa
     2221 gggctgctgc agggtggcgg gcatctcaaa tctcctgggc gctgcgcgcc agccgcaacg
     2281 tacgaaaccg tgccgtccgt gcgggggcca ccgcggaacc ggcattgccg ggccgctggt
     2341 ggcgccccct tgagggggag gcgccgaagg cgcttcgggg gtgggctttc atctcaggcc
     2401 cctttctcgt ggtccagcgc ggcggaggcc agcaactgga ggcacagttc ttcgtagctg
     2461 atgcccgccg cccgggcgga catcggcacc agcgaatgcc cggtcatgcc gggcgaggtg
     2521 ttgatctcca gcagccaggg cttgcgagtg gccgcatcga tcatcacgtc gacgcggccc
     2581 cagcc
//
LOCUS       DASWIY010000302         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_580918, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000302 DASWIY010000000
VERSION     DASWIY010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580918"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VGA59_11085"
     CDS             <1..1110
                     /locus_tag="VGA59_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9720265.1"
                     /translation="PLRYELAEMLADVMPGRALPRTNYEVSGTEAAEAAVHLALTHTQ
                     RRYVITFSSSYHGMSVGAKLLSGLGGEYGTYLEGWGGGVIKAPYPYSERIPAGMTEEQ
                     YTEYCLWYLDNHIPSCIAARDNIAAIMVEPGLAEGGNWIPNTRFLQGIREICDKNDWL
                     MIADEVLTGLGRTGRMWGIDHYSVVPDILVYGKNLCGGIAPLCGISARDDILGDNSEF
                     MSGSTFAGTPAGCAAAIKTLQIFERDKIVAHSHRLGGIAAEIMSGWEQYSIVRQARGN
                     GLLLGVSFGKPESAEPEAKDWWISRAVRGKMLENGVWAISDRQDTIRMYPALNMDEAV
                     LREGLQIMQDAIQHVEKHGHIEGDAAAWPSGVAGF"
     gene            1259..>2018
                     /locus_tag="VGA59_11090"
     CDS             1259..>2018
                     /locus_tag="VGA59_11090"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720266.1"
                     /translation="MNLFTIADKSAELPFRELVEQYGSPLLVLDCNVLRAGYRALQDA
                     LPGVDLFYAIKSLPERAALAALAAEGANFDIATSGEIELLRSIGADPAATIHTHPIKR
                     DQDIRDALDFGTTIFVADNIDELAKFEAYRSCARVLLRVSFRGKQVKCDLSRKFGCDP
                     ADIPWLLTQAAALGVEVVGLSFHVGSQSGDSDMHVAAIEACRAYFSDTSLAGTEHLHI
                     LDIGGGFPADYARSGFDIATFCRPIRDALASLPGY"
BASE COUNT          366 a          630 c          637 g          385 t
ORIGIN      
        1 ccgctgcgct acgaactggc cgaaatgctg gctgacgtga tgccgggccg ggccttacca
       61 cgcaccaatt acgaggtttc cggcaccgaa gctgccgaag cggcagtgca cctggcactc
      121 acccataccc agcgccgcta tgtcatcacc ttcagttcct cttaccacgg catgagtgtt
      181 ggcgccaagc tgctcagtgg tctgggtggt gagtacggca cgtatctgga aggctggggc
      241 gggggcgtga tcaaggcacc ttatccctac agcgagcgta ttccggccgg gatgaccgaa
      301 gagcagtaca ccgagtactg cctctggtac ctggataacc acattccttc gtgcatcgct
      361 gcacgggaca acatcgcggc catcatggtc gagccggggc tggcagaggg cggcaactgg
      421 attccgaata cgcggttcct gcagggtatt cgggagattt gcgacaagaa tgactggctg
      481 atgatcgccg atgaagtcct caccggcctt gggcgcacgg gccgtatgtg gggcatagac
      541 cattacagcg tggtgccgga cattctcgtg tacggcaaaa atctctgtgg cggcatcgcg
      601 cccttgtgcg gaatttctgc gcgtgacgac atactgggcg acaacagcga attcatgtcc
      661 ggttctactt ttgccggtac gccggcaggc tgcgcggcgg cgatcaagac cctgcagatc
      721 ttcgagcgcg acaaaattgt cgcacattcg catcgcctcg gtggcatcgc agccgagatc
      781 atgagtggat gggagcagta ctcgattgtc cgccaggcac gcggtaacgg cttgctgttg
      841 ggtgtgagtt ttggcaaacc cgagtcggca gaaccggaag ccaaggactg gtggatctcg
      901 cgtgccgtgc gcggcaagat gctggagaac ggcgtctggg ccatttccga ccgccaggac
      961 acgatccgca tgtatccggc gctcaatatg gacgaagcgg tgttgcgcga aggcttgcag
     1021 atcatgcaag acgccatcca gcacgtcgaa aaacacggtc acatcgaggg tgatgcagcg
     1081 gcttggccca gcggagtggc ggggttctag cttgttctcc aaaataacgc ttgcgtccgg
     1141 gcgcagaaga cctacaatgc tccgccggca tcgccgtccc cgccacgcgc catgtggccg
     1201 cgtggacacc ctgatactgt gcgcccgccc ccacgggctg taggaaacca ccacctggat
     1261 gaaccttttc acgattgcgg acaagtccgc tgaattgccg ttccgcgagc tggtagagca
     1321 gtacggctcg ccgctgctgg tgctggactg caatgtcctg cgcgccgggt accgtgccct
     1381 gcaggacgcg ctgccgggcg tcgacctgtt ctacgcgatc aagtccctgc cggagcgcgc
     1441 ggcgctggcc gccctggccg ccgaaggcgc caatttcgac atcgcgacca gcggcgagat
     1501 tgaattgctg cgcagcatcg gcgcggaccc ggccgccacc atccacacgc acccgatcaa
     1561 gcgcgaccag gacatccgcg atgccctgga tttcggcacg acgattttcg tcgcggacaa
     1621 cattgacgag ttggccaagt tcgaggccta ccggtcctgc gcgcgagtgc tgttgcgcgt
     1681 gtctttccgc ggcaagcagg tcaaatgcga cctgtcgcgc aagttcggtt gcgatcccgc
     1741 cgacatcccc tggctgttga cgcaggccgc cgcgctcggc gtcgaggtcg tgggcctgag
     1801 ttttcacgtc ggctcgcagt cgggcgacag cgatatgcac gtggctgcga tcgaggcctg
     1861 ccgcgcctat ttttccgata cctcgctggc cggcaccgag cacttgcaca ttctcgacat
     1921 cggcggcggc tttcctgccg attacgcgcg cagcgggttc gacatcgcaa cgttctgccg
     1981 gcccatccgc gacgcgctgg cgtcgttgcc cggctacg
//
LOCUS       DASWIY010000303         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2793906, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000303 DASWIY010000000
VERSION     DASWIY010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793906"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..731)
                     /locus_tag="VGA59_11095"
     CDS             complement(72..731)
                     /locus_tag="VGA59_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX9720267.1"
                     /translation="MTFRNPWLFGWIALLFAVALEAQDPAQENPGTTEDSAPSAAPKY
                     TPSYSEEGAASCLRCHAGGEMRAIQSGPHFNLQNPGAPAAHHYCESCHGPGSIHVSRA
                     HGGRGYPPLTEFGRGKDKASRDEQLAACMQCHGTAGEVRKTIGFSGSPHDRKAINCST
                     CHTLHAQSDPIKVREEQAETCYGCHRKMRAGHPRFEAESMNIQVLKCSDCHDVHRPLP
                     E"
     gene            complement(867..>1755)
                     /gene="arsB"
                     /locus_tag="VGA59_11100"
     CDS             complement(867..>1755)
                     /gene="arsB"
                     /locus_tag="VGA59_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX9720268.1"
                     /translation="WLMILPMLLKIDFHSLQEVRQHWKGVGVTLFINWAIKPFSMALL
                     AWIFIRNLFADWLPADQLDSYIAGLILLAAAPCTAMVFVWSNLSGGHPGFTLSQVALN
                     DVIMVFAFAPIVALLLGIASITVPWDTLLLSVVLYIVLPVIVAALWRRWLIRGADGHA
                     RLQRVLQRMHPVSLGALLLTLVLLFGFQGGQILARPVIILLLAIPILIQVYFNSGLAY
                     LLSRKLGVAHCVAAPSALIGASNFFELAVAAAISLFGLESGAALATVVGVLVEVPVML
                     SVVWIVNRSKPWYEAALRT"
BASE COUNT          331 a          561 c          585 g          278 t
ORIGIN      
        1 cgggtaaacc acctgctcgg gttcgcccgc gatgggaacg tcgaatccct tgcgtatacg
       61 aatggtccgg gtcattcggg cagcggacgg tgcacgtcgt ggcagtccga gcacttcagc
      121 acctggatgt tcatgctctc ggcttcgaac cgggggtgcc cggctcgcat cttgcgatgg
      181 caaccgtagc aggtttcggc ctgctcctcg cgaaccttga tcgggtcgga ctgcgcatgg
      241 agcgtgtggc aggtcgagca gttaatggct ttgcggtcgt gcgggctgcc ggaaaagccg
      301 atggtcttgc gcacttcgcc cgcggtcccg tggcattgca tgcaggccgc caactgctcg
      361 tcgcgcgacg ccttgtcctt gccgcggccg aattcggtca gcggcgggta accgcggccg
      421 ccatgcgccc gcgagacgtg gatgctgccg ggcccgtgac aggactcgca gtaatggtgc
      481 gcggccgggg cgccggggtt ctgcaggttg aagtgcgggc cggactgaat ggcccgcatt
      541 tcgccacccg cgtggcagcg caggcagctc gccgcgccct cctcgctgta actcggcgtg
      601 tatttcggcg cggcggaagg ggccgaatcc tcagtcgtgc ccggattctc ctgggccgga
      661 tcctgcgcct cgagcgccac cgcgaacagc agtgcaatcc agccgaaaag ccatggattt
      721 ctgaaagtca tttcgggccc cgaatccctc tcgagtttgg agccccaatg ctacaccgtg
      781 gcatctcagg cttgaacggg agcggatgat cagggtcaag cccggcgcgg gtcgctgcga
      841 aaattcggcg gcaaacttga cctttgtcag gtccgtaggg ctgcctcgta ccacggtttg
      901 ctgcggttga cgatccagac cacggagagc atcacgggca cctcgaccag cacgcccacc
      961 acggtggcca gggcggcgcc ggattccaga ccgaacaggc tgatggcggc ggccaccgcc
     1021 agctcgaaga agttgctggc gccgatcagc gccgaaggcg ccgctacgca gtgggccaca
     1081 cccagcttgc gactgagcag gtaggccagg ccggagttga aatagacctg gatcaggatg
     1141 gggatcgcca gcagcaggat aatcaccggg cgggccagga tctggccgcc ctggaagccg
     1201 aacagcagca ccagcgtcag caacagcgcg cccagcgaca ccgggtgcat gcgttgcagc
     1261 acgcgctgca gtcgcgcgtg cccgtccgcg ccacgtatca accagcgccg ccacagcgcc
     1321 gcgacgatca ccggcagcac gatgtacagc accaccgaca acaacagcgt gtcccacggc
     1381 accgtgatgg aggcgatgcc cagcagcagg gcgacgatcg gcgcgaaggc aaagaccatg
     1441 atcacgtcgt tcagcgccac ctgcgacagg gtgaagccgg ggtggccgcc actgaggttg
     1501 ctccagacga acaccatcgc cgtgcacggg gccgccgcga gcaggatcag gccggcgatg
     1561 tagctgtcga gctggtcggc cggcagccag tccgcgaaca ggttacggat gaagatccag
     1621 gccagcaaag ccatggagaa cggtttgatg gcccaattga tgaacagggt gacgccgacg
     1681 cccttccagt gctgccggac ttcctgcagc gaatgaaagt cgatcttcag caacatgggc
     1741 aggatcatca gccag
//
LOCUS       DASWIY010000304         4425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1695520, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000304 DASWIY010000000
VERSION     DASWIY010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695520"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VGA59_11105"
     CDS             <1..583
                     /locus_tag="VGA59_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX9720269.1"
                     /translation="TATHEALSAALPGVMVGLLHSRMPAPEKKAVMSLFTGGQMGVLV
                     ATTVIEVGVDVPNASLMVVEHAERFGLSQLHQLRGRVGRGAAASACILLYSAGEGGRL
                     GETARARLKAMVETADGFEIARRDLEIRGPGEFLGARQSGSPLLRFADLATDTALLDW
                     ARKLAPLMLDRHPELAKRHTARWLGGKSEYLKA"
     gene            716..1669
                     /locus_tag="VGA59_11110"
     CDS             716..1669
                     /locus_tag="VGA59_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9720270.1"
                     /translation="MTLTELKYIVAVAREKHFGKAAEACFVSQPTLSVAIKKLEDELE
                     VKLFERSANEVTVTPLGEEIVRQAQSVLEQAASIKEIAKRGKDPLAGPLKLGIIYTIG
                     PYLLPDLVRQAIARTPQMPLMLQENFTVKLLEMLRTGDIDCAIMAEPFPDTGLAVAQL
                     YDEPFLAAVPSSHPLAANKTITSQELKSETMLLLGTGHCFRDHVLEVCPEFARFSSDA
                     EGIRKSFEGSSLETIKHMVAAGMGVTLVPRLSVPKEAFEARPKRRKEDEAYIRYLPFD
                     GDPPTRRVVLAWRRSFTRYEAIAALRNAIYACELPGVKRLS"
     gene            1695..2612
                     /locus_tag="VGA59_11115"
     CDS             1695..2612
                     /locus_tag="VGA59_11115"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006753361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEX9720271.1"
                     /translation="MKDMKPYQPGIIEPVPAVARYVLFNLAETGADAQAIKDAFTRIS
                     PLANGSDVVLGIGPSLVEALGARIPGLHPFPDFSGHGVKVPATPGTLWCWVRGDDLGD
                     LLHLTRRVQKALAPVFVVRHVVDAFRHAVGESGHGKDLTGYEDGTENPQGEAAEEAAF
                     AHGLGEGLDGSSYVAVQQWVHDLDAFDALAREEQDHHFGRRRSDNEELDDSPTWAHVK
                     RTAQESFDPPAFILRRSMPWMMSMQAGLMFVAFGKSLDAFEAQMRRMAGRDDGITDAM
                     FRISKPVSGAYYWCPPMRDGKLDLRKLGL"
     gene            2612..3472
                     /gene="ubiA"
                     /locus_tag="VGA59_11120"
     CDS             2612..3472
                     /gene="ubiA"
                     /locus_tag="VGA59_11120"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX9720272.1"
                     /translation="MFGEKLRLYLDLIRWNRPAGWLLLLWPSLSALWIAAHGFPGWHL
                     LVIFTLGTILMRSAGCCVNDVADREFDRHVKRTAQRPVTRGAVSVREALLLGAVLALA
                     AFGLVLTTNAAAVSWSFAALAIAVAYPYAKRFVSMPQAVLGVAFSFGIPMAFAAVQSR
                     VPLLAWVLLLGNLFWVLAYDTEYAMVDRDDDLKIGMKTSAITLGRADVPVIMLCYASY
                     LCIWAVALAGRIPLPVILIATAAAAGQAIWHFVLIRDRDREGCFKAFRLNHWLGLTMF
                     AGIVAGFALK"
     gene            complement(3465..4325)
                     /gene="proC"
                     /locus_tag="VGA59_11125"
     CDS             complement(3465..4325)
                     /gene="proC"
                     /locus_tag="VGA59_11125"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX9720273.1"
                     /translation="MTSRTALTSKEPIAFIGGGNMASAIIGGLIRQGLPATEIEVVEP
                     FAPAREKLKDQFGIAAQEHAGAALVRAGLVVWAVKPQTFREAAVQARAHTTAALHLSV
                     AAGIRSDSIAQWLGTERIVRSMPNTPALIGKGIAALYARAGVSAQDQSRVEEIIATTG
                     ESLWVQEEAQLDAVTALSGSGPAYVFFFLEAMTRAGTEMGLPRAQAYRLAVATFAGAA
                     ELARASEDPPEVLRQRVTSKGGTTHAAIGSMEQDQVQALFVKALHAARQRARELGDEF
                     GGQAGLAPPT"
BASE COUNT          700 a         1464 c         1498 g          763 t
ORIGIN      
        1 gacggccacg cacgaggcgc tgagcgccgc cctgccaggc gtcatggtcg gcctgctgca
       61 ttcgcgcatg ccggcgccgg aaaaaaaggc cgttatgtcg ctcttcaccg gcggccagat
      121 gggcgtgctc gtcgccacca ccgtcatcga ggtgggcgtg gacgtaccca acgcgtcgct
      181 gatggtggtc gagcacgccg agcgcttcgg gttgtcgcag cttcaccagc tgcgcgggcg
      241 ggtcggccgc ggtgccgcgg cgtcggcctg catccttctt tactcggcgg gggaaggcgg
      301 ccgactgggc gagacggccc gcgcccggct gaaagccatg gtcgaaaccg cggacggctt
      361 cgagatcgcg cggcgcgacc tggagattcg cggtcccggc gagttcctgg gcgcccgcca
      421 gtcgggttcc ccgctgcttc ggttcgccga cctggccacc gacaccgcgt tgttggactg
      481 ggcccgcaag ctcgccccgc tgatgctcga tcggcacccc gagctggcta aacgccacac
      541 ggcgcgctgg ttgggtggaa aatccgaata cctcaaggcc tgacgcaaga atcccaatga
      601 cgctcactga gctcaagtac atcgtaatcc tggaaaggaa agggcgaggg gccccgcttg
      661 cggggatcga cccctggaca ggattggcga cggcctttgg aaaaggtgtc tcgcgatgac
      721 cctcaccgag ctgaagtaca tcgtcgccgt cgcgagggag aagcacttcg gcaaggccgc
      781 cgaagcctgc ttcgtatcgc agccgacgct gtccgtggcg atcaagaagc tcgaggacga
      841 gttggaggtg aagctcttcg agcgcagcgc caacgaggtg acggtgaccc ccctgggcga
      901 ggagatcgtg cggcaggcgc agagcgtgct ggaacaggcg gccagcatca aggagatcgc
      961 caagcgcggc aaggacccgc tggcgggccc cctcaagttg ggcatcatct acaccatcgg
     1021 cccctacctg ctgccggacc tggttcgcca ggcgatcgcg cgaacgccgc agatgcccct
     1081 gatgctgcag gaaaacttca ccgtcaagct gctcgagatg ctgcgcaccg gcgatatcga
     1141 ttgcgccatc atggccgagc ctttcccgga taccgggctg gccgtggccc agctctacga
     1201 cgagccgttc ctcgcggccg tgcccagcag ccatccgctg gccgcgaaca agaccatcac
     1261 ctcccaggag ctcaagagcg aaacgatgct gctgctgggc accggccatt gtttccgcga
     1321 ccatgtcctg gaggtgtgcc ccgagttcgc gcgcttttcc agcgatgccg agggcatccg
     1381 caagagtttc gaaggctcat ccctggagac gatcaagcac atggtggccg ccggcatggg
     1441 tgttacgctg gtgccgcggc tgtcggtgcc caaggaagca ttcgaagcgc ggcccaagcg
     1501 gcgcaaggaa gacgaggcct acatccgcta ccttcccttc gacggcgacc cgccgacccg
     1561 gcgcgtcgtg ctggcctggc gccgcagctt cacccgctac gaggcgatcg ccgcgctgcg
     1621 caatgccatc tatgcttgcg aactgccggg cgtcaaacgc ctgtcctgag ttcttcaatc
     1681 ccttgtttgg aaccatgaaa gacatgaagc cctaccagcc cggaatcatc gaaccggtgc
     1741 cggccgtcgc tcgctatgtg ctgttcaacc tcgccgaaac cggcgcggac gcccaggcca
     1801 tcaaggatgc gttcacccga atctcgcccc tggccaatgg cagcgacgtg gtgctcggca
     1861 tcggcccgtc gctggtggag gcgctgggtg ccaggattcc cggcctgcac ccctttccgg
     1921 acttctccgg ccatggcgtc aaggttcccg ccacgccggg aacgctctgg tgctgggtgc
     1981 gcggagacga cctgggcgac ttgttgcacc tgacacgacg agtccagaag gcgttggcgc
     2041 cggtgttcgt ggtgcgccac gtggtggacg cgtttcgcca cgcagtgggc gagagtggcc
     2101 atggcaagga cctgaccggt tacgaggacg gcacggaaaa cccgcagggc gaggcggccg
     2161 aggaagccgc attcgcgcac ggcctggggg aagggctgga cggctcgagc tatgtggcgg
     2221 ttcagcagtg ggtgcacgac ctcgacgctt tcgacgccct tgcccgcgaa gagcaggacc
     2281 accacttcgg ccggcgccgc agcgacaacg aggaactgga cgattcgccg acctgggccc
     2341 acgtcaagcg aaccgcgcag gaaagcttcg atccgccggc tttcatcctg cgccgctcca
     2401 tgccctggat gatgagcatg caggcgggcc tgatgttcgt ggctttcggc aagtcgttgg
     2461 acgccttcga agcccagatg cggcgcatgg cgggacgcga tgacggcatc accgacgcga
     2521 tgttccgcat ctccaaaccg gtttccggtg cgtactactg gtgcccgccg atgcgggacg
     2581 gcaagttgga cctgcgcaag ctcggcctgt gatgtttggc gaaaagctgc gtctttatct
     2641 ggacctgatc cgctggaacc gtccggcggg ttggctgctg ctgctgtggc cgtcgctcag
     2701 tgccctgtgg atcgccgccc atggcttccc cggttggcac ctgctggtga tcttcacgct
     2761 gggaaccatc ctcatgcgca gcgccggctg ctgcgtcaac gacgtggcgg atcgcgagtt
     2821 cgaccgccat gtgaagagga cggcacaaag gccggtcacc cgcggcgcgg tctcggttcg
     2881 cgaagccttg ttgctggggg cggtgctggc gctggccgcg ttcggcctgg tgctgaccac
     2941 caatgcggcg gcggtctcct ggtcgttcgc cgccctggcg atcgccgtgg cctatcccta
     3001 cgccaagcgc ttcgtctcca tgccgcaggc ggtgctgggc gtggcgttca gcttcggcat
     3061 cccgatggcc ttcgccgccg tgcaatcacg ggtgccgctg ctggcctggg tgctgttgct
     3121 gggtaacctg ttctgggtac tggcctacga caccgagtac gcgatggtcg atcgcgacga
     3181 cgacctgaag atcggaatga agacctcggc catcacgctg ggccgcgccg acgtgccggt
     3241 gatcatgctc tgctatgcga gctacctttg catctgggcg gtggccctgg ccgggcgcat
     3301 cccgctgccg gtgattctga tcgccacggc cgcggccgcc gggcaggcga tatggcattt
     3361 cgtgctgatt cgcgaccgcg accgcgaggg ttgcttcaag gcgttccgcc tcaaccattg
     3421 gctgggcctc acgatgtttg ccggcatcgt ggccggcttc gcgctcaagt aggtggcgcc
     3481 aagccggcct ggccgccgaa ttcgtcgccc agctcccgcg cgcgctgccg ggccgcatgc
     3541 aaggccttga cgaagagcgc ctggacctgg tcctgttcca tggaaccgat ggcggcgtgc
     3601 gtggtgccac ccttggacgt cacccgctgt cgcagcactt cggggggatc ttcggaggcc
     3661 cgcgccagct cggccgcgcc ggcaaaggtg gccacggcaa gccggtaggc ctgcgcgcgg
     3721 ggcaacccca tttcagtgcc ggcccgggtc atggcctcca ggaagaagaa cacataggcc
     3781 gggccggaac ccgagagggc ggtgacggca tccagctgcg cctcttcctg cacccacagc
     3841 gattcgccgg tcgtggcgat gatttcctcc acccgtgact ggtcctgggc ggaaacgccc
     3901 gccctggcat aaagcgccgc gatgcccttg ccgatcagcg cgggcgtgtt gggcatggag
     3961 cgcacgatgc gctcggtgcc cagccattgc gcgatgctgt cggagcggat tccggcggct
     4021 acgctcaggt gcagcgccgc cgttgtgtgg gcccgcgcct ggaccgcggc ctccctgaag
     4081 gtctggggct tgaccgccca gaccaccaag ccggcgcgca ccagcgccgc gcccgcgtgc
     4141 tcctgcgccg cgatgccgaa ctggtccttg agcttctcgc gggccggggc aaacggctcc
     4201 accacctcga tctcggtggc gggcaggccc tggcggatca ggccgccgat gatggcgctg
     4261 gccatgttgc cgccaccgat gaaggcaatg ggctctttgg aagtgagggc tgtcctgctt
     4321 gtcatggccg cagtctatcg ctgcggcctt ccggtggtgc gtcaggcgtc ccgcggctga
     4381 ggtcggtgag aatagggtaa cagtgtgaac gggccctcga tgaac
//
LOCUS       DASWIY010000305         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_198115, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000305 DASWIY010000000
VERSION     DASWIY010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198115"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="VGA59_11130"
     CDS             <1..1877
                     /locus_tag="VGA59_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX9720274.1"
                     /translation="DVARAQPAMTGPRVPPPVLVRGRIDKTLVIDTLIPPDPGKFSWA
                     GHLGARMKQPVVDEIARSGTTLVFTNVRSQAEIWYQLLLEAKPEWAGEIALHHGSMDK
                     ATREWVEQGLKQGSLRAVVATSSLDLGVDFLPVERVLQIGSAKGVARMMQRAGRSGHA
                     PGRASRVTLVPTNTMEIIEAAAARRAACAGRVEQRESPDKPLDVLVQHLVTIALGGGF
                     RAGELFDEVRTAWAYRGLTREEFDWALSFCERGGDSLTAYPDYHRITQDEDGVYRVRD
                     RGIAKRHRLGIGTIVSDASMQVKYMSGANIGTIEEGFIARLRKGDCFYFAGKLLEFIR
                     VQEMAAYVRRATRNKGTVPTWQGSKMALSAELGDAVLEMMQRAAQGDFVEPELEAARP
                     MLQTQARLSRIPTPDTVLVETFRSREGHHLYVYPFAGRHVHLGLASLLAWRLARERPN
                     TFSISVNDYGFELVAAGQFDLAPVMDKSVFSTADLLHDVLASLNSTELAQRRFREIAR
                     VAGLVFQGYPGQPKSTRQLQASSGLFFEVFRKYDKGNLLLGQAEREVLSQELEISRLR
                     ATLTRLARKKLEHVELRHPSPMSLPLMVERFREQLTTEQLSRRLDRMLGDMERDAAGA
                     "
     gene            1994..2446
                     /locus_tag="VGA59_11135"
     CDS             1994..2446
                     /locus_tag="VGA59_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX9720275.1"
                     /translation="MKPFHQKSLMLASSLAMLLALGACDRYGDQSAGKTPADSAGQAT
                     SQATEESRDAAARARAAADKAGEETKAMGAAAVDKVDDATITTKVNAELATDKDLSAI
                     KIDVDTKDGVVTLTGPAPSADARERATEIAKNVKGVTSVNNQLTVKAG"
BASE COUNT          447 a          824 c          870 g          375 t
ORIGIN      
        1 cggacgttgc gcgagcccag cccgcaatga caggcccccg ggtgcccccg ccggttctcg
       61 tgcgcgggcg catcgacaaa accctcgtga tcgacacgct cataccgccc gaccccggca
      121 agttctcgtg ggcggggcac ctgggcgcgc gcatgaagca gccggtggtc gacgagatcg
      181 caagatcggg caccaccctg gtgttcacca acgttcgctc gcaagccgag atctggtacc
      241 agctgctgct cgaggccaag cccgagtggg ccggcgaaat cgccctgcat catggctcga
      301 tggacaaggc cacgcgcgaa tgggtggagc agggcttgaa gcaaggcagt ttgcgggccg
      361 tggtcgccac gtcgtcgctg gacctgggcg tggacttcct gccggtggaa cgcgtgctgc
      421 agatcggctc ggcaaaaggc gtggcgcgca tgatgcagcg ggccggccgc agcggccatg
      481 cgcccggacg cgcgagccgc gtgacgctgg tgcccaccaa cacgatggag atcatcgagg
      541 cggcggcggc gcgccgcgcc gcttgcgccg gccgggtgga acagcgcgag tccccggaca
      601 agccgctgga cgtgctggtc cagcatctgg tcaccattgc gctgggcggc gggtttcgcg
      661 ccggcgagct gttcgacgag gtccgcaccg cctgggccta tcgcggtctc acgcgcgagg
      721 aattcgattg ggcgctgtcg ttctgcgaac gtggcgggga cagtcttacg gcttatcccg
      781 attaccaccg catcacgcag gacgaggacg gcgtctaccg cgtgcgcgac cggggcatcg
      841 ccaagcgcca tcgcctgggc ataggcacga tcgtgagcga cgcctcgatg caggtgaagt
      901 acatgagcgg cgccaacatc ggcacgatcg aggagggctt catcgcgcgg ctgcgcaagg
      961 gcgactgttt ctacttcgcc ggcaagctgc tggagttcat ccgggtgcag gagatggctg
     1021 catacgtcag gagggccacg cgcaacaagg gcacggtgcc gacctggcag ggcagcaaga
     1081 tggcgctgtc cgccgagctg ggcgacgcgg tgctggaaat gatgcaaagg gcggcgcagg
     1141 gcgatttcgt cgaacccgaa ctcgaggcgg cccggcccat gctgcagacg caggcgcggc
     1201 tgtcgcgcat tcccacgccc gacacggtgc tggtcgagac cttccgctcg cgcgagggtc
     1261 atcacctcta cgtttacccg ttcgccggcc ggcatgtgca cctggggctg gcgagcctgc
     1321 tggcgtggcg cctggcgcgc gagcggccca acacgttcag catttcggtc aatgactacg
     1381 gcttcgagct ggtggccgcc gggcagttcg atctggcgcc ggtcatggac aagagcgtgt
     1441 tctccacggc ggacctgctg cacgatgtgc tggcttcgct caactccacc gagctggcgc
     1501 agcggcggtt tcgcgagatc gcgcgcgtgg ccgggctggt ttttcaaggc taccccggcc
     1561 agcccaagag cacccggcag ctgcaggcct ccagcgggct ttttttcgag gtgtttcgca
     1621 agtacgacaa gggcaacctg ctgctcggcc aggccgagcg tgaagtgctg agccaggagc
     1681 tggagatttc gcgcctgcgc gccacgctga cccgcctcgc ccgcaagaag ctggaacatg
     1741 tggaactgcg ccatcccagc ccgatgagcc tgccgctgat ggtcgagcgc tttcgcgagc
     1801 agctcaccac cgaacagctg tccaggcgcc tggaccgaat gctgggtgac atggagcgtg
     1861 acgccgccgg cgcctgatcc gtcctacagc gggtgcggcg ctgtccgact gccgcgcccc
     1921 ggccaggggc ttataaattg aagggcactc gggcgttggc gcccgtcgat ccacattcac
     1981 cgaaaggaac accatgaaac ccttccacca gaaaagcctg atgctggcct cgtcgctggc
     2041 gatgctgctc gcactgggcg catgcgaccg ctatggcgac cagtcagccg gcaagacgcc
     2101 tgcggatagc gccgggcagg ccaccagcca ggcgacggaa gagtcacgcg atgcggcggc
     2161 ccgagcccgc gccgcggcgg acaaagccgg cgaagaaacc aaggccatgg gcgccgcggc
     2221 cgtcgacaag gtggacgatg ccaccatcac caccaaagtg aatgcggagc tggccaccga
     2281 caaggacctg tcggcgatca agatcgatgt ggacacgaag gacggcgtgg tcaccctgac
     2341 cggcccggcg ccttcggccg acgcgcgcga gcgggcgact gaaatcgcca agaacgtcaa
     2401 gggtgtgaca tcggtcaaca accagttgac ggtcaaggct ggatgagcca gcagcgggcc
     2461 aacacaccat caccgccacg gcccgtttga ccggatcgag ggccaccttc gggtgg
//
LOCUS       DASWIY010000306         8012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_942292, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000306 DASWIY010000000
VERSION     DASWIY010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942292"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..964)
                     /locus_tag="VGA59_11140"
     CDS             complement(284..964)
                     /locus_tag="VGA59_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9720276.1"
                     /translation="MKLLSCLLTAALVATASSAFAAEEKPAAPAAGAAAAAPGAPAVP
                     APAKPDLAQGQAQYAAVCAACHGPDGNSGVPANPKLANQHPEYLVKQLQEFKNGKRPS
                     PIMQGFASQLSDADMKNIAYWIGAQKAKPGFAKDKELVALGERIYRGGIGERQVAACA
                     GCHSPNGAGVPSQFPRVSGQHADYTTAQLLAFRDGARKNSLLMSQVAAKLSDREIKAV
                     ADYIAGLR"
     gene            1047..1727
                     /gene="yihA"
                     /locus_tag="VGA59_11145"
     CDS             1047..1727
                     /gene="yihA"
                     /locus_tag="VGA59_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX9720277.1"
                     /translation="MTSAPGQPSGTKTAAALAVGWMHTARFLTTAPKLEFLPTMSVPE
                     IAFVGRSNAGKSTCINTLTQQKRLAFASKTPGRTQSINLFALGKQGITDAVLADLPGY
                     GYAAVPKQDKLRWQQVMASYLVTRENLRAVVLMCDPRLGLTELDDILLEVIRPRVEQG
                     LKFLLLLTKADKLNRSEQAKALSIARLQAGGGEVKLFSALKKQGVEQSSLLLWQWAHP
                     ETAEPPSQ"
     gene            1724..2614
                     /locus_tag="VGA59_11150"
     CDS             1724..2614
                     /locus_tag="VGA59_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9720278.1"
                     /translation="MIEQFQQDLPHGITLSCRAAGQVGRPTLVFLHGFPEGAFVWDGL
                     LEHFARPENGGYRCVAPNLRGYEKSSQPADVKAYRVKFLLQDIAALIRAEGAPLECLV
                     AHDWGGAVAWNLANQLPELTKKLAIINSPHPGTFLRELQSSPSQRAASAYMNFLIRPD
                     AEALLREDDYRRLWAFFTSWGPAAWLTEPLKQQYREVWDASLAGGCNYYRASPLRPPR
                     PEDPGAAAITLPHEMLTVSLPTLVLWGMDDAALPPALVDGLEQYVPRLTLEKVAGAGH
                     WIVHERPQFVAQRLAGFLRR"
     gene            complement(2632..3495)
                     /locus_tag="VGA59_11155"
     CDS             complement(2632..3495)
                     /locus_tag="VGA59_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HEX9720279.1"
                     /translation="MSELGILFMRLLAHLPLAWIRALGWVLGWFLYLVVVPRRHVVQT
                     NLALCFPHWSARRRRALVPRIFVSVAQAWLDRSWLWHGKPERTRRRLTLAGAVEELEG
                     LAPTVIFAPHFVGMDAGWTALTQQVQRDFTGIYTGQSNKVVDAWMLVGRRRFSSGRPF
                     GRADGVKAIVSALRGGAPLYLLPDMNFGPEESIFVPFYGIEAATVPSLSRFARLGRAK
                     VVPVVTRMTREGYEVGVLPAWKDFPSDDAARDTALMNERLQGYIDGMPEQYYWVHKRF
                     KTRPPGMPQVY"
     gene            complement(3492..4319)
                     /locus_tag="VGA59_11160"
     CDS             complement(3492..4319)
                     /locus_tag="VGA59_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9720280.1"
                     /translation="MKTLFRVFALLPLPVLHAVGAALGWVSFLASSTYRRRFTDNAQQ
                     AGYTLAQVRSAIAEAGKLVAETPRLWFGRSVAIHWEGAELVDMARAHGRGIVFLTPHL
                     GCFEVTAQGYAMRYGRITVLYRPARKPWLRDLVDTARDRANLATAPTTLGGVKQLLKA
                     LKAGEAVGLLPDQVPPEGLGVWAPFFGRAAYTMTLPARLAQQTGATVLLAWGERLPWG
                     RGYRIRLRPWPGELPSEAADAAARVNAQMEHLIRERPHQYLWGYARYKAPAGGSSRP"
     gene            4454..5620
                     /gene="metK"
                     /locus_tag="VGA59_11165"
     CDS             4454..5620
                     /gene="metK"
                     /locus_tag="VGA59_11165"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX9720281.1"
                     /translation="MANDFLFTSESVSEGHPDKVSDQISDAILDAVFAQDPRSRVAAE
                     TLCNTGLVVLAGEITTNAHVDYIQVARDTITRIGYDNTEYGIDYKGCAVLVAYDKQSP
                     DIAQGVDRASDDYLNQGAGDQGLMFGYACSETDTLMPLPIYLSHRIVERQAQLRHDGR
                     LPWLRPDAKSQVTIKYVDGKPHSIDTVLLSTQHAPEMTLEQIREAAIEEIIKPVLPAE
                     LIKGDIKFLVNPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDR
                     SAAYAGRYVAKNIVAAGLADKCLVQVSYAIGVAQPTSIMVSTFGTGHISDEKITELVK
                     KHFDLRPKGIVQMLDLLRPIYQNTAAYGHFGREEPGFTWENTDKAAALRADAGL"
     gene            6017..7243
                     /locus_tag="VGA59_11170"
     CDS             6017..7243
                     /locus_tag="VGA59_11170"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX9720282.1"
                     /translation="MLKRIPVEQLRLGMFVHEFCGSWMEHPFWRGAFLLDDPKDLETI
                     LSTGLKEVWIDTSRGQDVEGGATREAVAAEVEAALVQVEPGKMVPRTAVAQEAVRAVK
                     ICAQSKQAVASMFHEARMGKALDVGDALPIVEEISTSVMRNPGALISLARLKNKDEYT
                     YMHSVAVCALMVSLARTLGFNDQQTRQAGLAGLLHDVGKMMIPLSILDKPGKLTEAEF
                     ETMKSHPVEGHRMLLGGTGIGEVALDVCLHHHEKMDGSGYPHGLGGGEISQYARMGAV
                     CDVYDAITSNRPYKNGWEPAESIRKMAEWSKGHFDPRIFEAFVRSIGIYPIGSLVRLA
                     SDHLGVVVEQTEQSLLTPIVMVFFSIKSDARIAPELLNLSKPGCKDRIVSHEDPVKWD
                     IKDTQALWSGMDVAPW"
     regulatory      7285..7360
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            7385..>8012
                     /locus_tag="VGA59_11175"
     CDS             7385..>8012
                     /locus_tag="VGA59_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX9720283.1"
                     /translation="MNAVAFNDYVIADIKLADWGRKEIAIAEHEMPGLMAIREEFAKT
                     QPLKGARITGSLHMTIQTAVLIETLTALGAEVRWASCNIFSTQDHAAAAIAADGIPVF
                     AVKGETLAEYWDYTHRIFEWADGGYSNMILDDGGDATLLLHLGARAETDASLISKPGS
                     EEETCLFAAIKAKLATDPTWYSTRLAQIKGVTEETTTGVHRLYQMHQRG"
BASE COUNT         1471 a         2641 c         2605 g         1295 t
ORIGIN      
        1 tccgccgctc ctgccatgtt ccatggctgt gcagggcgca taggcgccga tgaaggggca
       61 gtcgggcgaa ccccggcgac aaaaagaaaa gcggggcctc gcggccggct agttcatctg
      121 gtccattccg ctgcgcaccc gcgacgtatg aaatcgcgcg gacagtgcgg ggccaccgcg
      181 gaaccggctt tgccgggccg ctggtggcgc ccccttctat gagggggaga gggcgaagcc
      241 cgagggggca tcgccgaagg cgcttcgggg gtgggctttc acttcagcgc aggccggcga
      301 tgtagtcggc aaccgctttg atttcgcggt cgctgagctt ggccgcgacc tgcgacatca
      361 gcaagctgtt cttgcgagcg ccgtcccgga aagccagcag ctgagccgtc gtgtagtcgg
      421 catgctggcc cgacacgcgc gggaactggg aaggaacgcc cgctccgttc gggctgtgac
      481 agccggcgca ggcggccacc tggcgctcgc cgatgccgcc gcggtagatg cgttcaccca
      541 gggccacgag ctccttgtct ttcgcgaaac ccggcttggc tttctgcgcg ccgatccagt
      601 aggcgatgtt tttcatgtcg gcgtccgaca gctgggacgc aaagccctgc atgatcgggc
      661 tcgggcgctt gccgttcttg aactcctgca gttgcttgac caggtattcc gggtgctggt
      721 tagccagctt ggggttggcc ggcacgcccg agttgccgtc cgggccatgg caggcggcgc
      781 acacggccgc gtactgcgcc tggccttgcg ccaggtccgg cttggcggga gccgggaccg
      841 ccggcgcccc cggcgcagcc gccgcagccc ccgctgcggg agcggcaggc ttttcctccg
      901 cagcaaaggc ggacgaggcg gtcgccacca gggcagccgt caagaggcag gaaagcaact
      961 tcattgtcag gcgtcggatt gttggggcaa agccggtaat tctacaatgc cgcactcggg
     1021 tttccccgca aacgaaaagg cacctcatga cctccgcccc cgggcaaccc agcggcacca
     1081 agaccgcagc agcgctcgcc gtcggctgga tgcacacggc ccgctttctc accacggcgc
     1141 ccaagctcga gttcctgccc acgatgagcg ttcccgagat cgctttcgtc ggccgatcca
     1201 acgcgggcaa atccacctgc atcaacacgt tgacccagca aaaacggctg gcgttcgcct
     1261 cgaagacgcc gggtcgcacg cagagcatca acctgttcgc gctgggcaag cagggcatca
     1321 ccgacgccgt gctggccgac ctgcccggct atggctatgc ggccgtgccc aagcaggaca
     1381 agctgcgctg gcagcaggtg atggccagct acctggtcac gcgcgagaac ctgcgcgccg
     1441 tggtgctgat gtgcgacccc cgcctggggc tgaccgaact cgacgacatc ctgctggaag
     1501 tgatccgccc ccgggtcgag caaggcctga agttcctgct gctgctgacc aaggcggaca
     1561 agctcaaccg cagcgagcag gccaaggccc tgtccattgc gcgactgcaa gccggcggcg
     1621 gcgaggtcaa actgttctcc gccctgaaga agcagggcgt cgaacaatcg tccctgctgc
     1681 tgtggcagtg ggcacatccc gagaccgccg agcctcccag ccaatgatcg aacagtttca
     1741 gcaagacctg ccgcacggaa tcaccctgag ctgccgcgcc gccggccagg tcggccggcc
     1801 caccctggtg ttcctgcatg gctttccgga aggcgccttc gtctgggacg gcctgctcga
     1861 gcatttcgcc cggcccgaga acgggggcta tcgctgcgtg gcgcccaacc tgcgcggcta
     1921 cgagaaatcc agccagccgg ccgatgtcaa ggcctaccgc gtcaaattcc tgttgcagga
     1981 catcgccgcg ctgattcggg ccgagggtgc gcccctcgaa tgcctggtgg cgcacgactg
     2041 gggcggcgcg gtcgcctgga acctcgcgaa ccagttgccg gaactcacga agaagctcgc
     2101 catcatcaac tcgcctcatc cggggacgtt cctgcgcgag ctccagtcca gtcccagcca
     2161 gcgggccgcc agcgcctaca tgaacttcct gatccgcccg gatgccgagg cgctgctgcg
     2221 cgaagacgat taccgcaggc tgtgggcctt cttcaccagc tggggcccgg cggcctggct
     2281 gaccgaaccg ctcaagcagc agtaccgcga ggtctgggac gccagcctcg ccggcggctg
     2341 caactactac cgggcctcgc cgctgcggcc gccccggccg gaagaccccg gcgcggcggc
     2401 gatcacgctg ccgcatgaga tgctcaccgt gagcctgccc accctggtgc tgtggggcat
     2461 ggatgacgcc gcgctgccgc cggcactggt ggacggactg gagcagtacg tgccgcgact
     2521 cacgctggaa aaagtggcgg gggccggcca ttggatcgtg cacgagcggc cgcaattcgt
     2581 ggcgcagcgg ctcgccgggt tcctgcggcg ctgaggccgc gccacgcaga ctcaatagac
     2641 ctggggcatg cccgggggtc gcgtcttgaa gcgcttgtgc acccagtagt actgctcggg
     2701 catgccgtcg atgtagccct gcaagcgctc attcatgagc gccgtatcgc gcgcggcgtc
     2761 gtccgaaggg aaatccttcc aagccggcaa cacccccacc tcgtagcctt cacgcgtcat
     2821 tcgtgtgaca accggcacca ccttggcgcg gcccagcctg gcaaaacggg agagcgaggg
     2881 cacggtggcg gcctcgatgc catagaaggg cacgaagatc gactcctccg gcccgaagtt
     2941 catgtcgggc aacaggtaca gcggcgcgcc gcctcgcagc gccgagacga tggccttgac
     3001 gccatcggcc cggccgaacg ggcggccgga actgaaacgc cgccggccaa ccagcatcca
     3061 cgcatccacc accttgttcg attggccggt gtagatgccg gtgaagtctc gctgcacctg
     3121 ctgcgtcagt gcggtccagc ccgcgtccat ccctacgaag tggggggcga agatcaccgt
     3181 cggcgccagg ccttcgagtt cctcgacggc gcccgccagc gtgagccgcc gccgtgtgcg
     3241 ttcgggcttg ccatgccaca gccagctgcg atcgagccac gcctgggcca cgctcacgaa
     3301 aattcgcggc accagcgcgc ggcgccggcg agcgctccaa tgcggaaaac acagcgccag
     3361 gttggtctgg acgacatggc ggcgcggaac caccaccagg tagaggaacc agccgagcac
     3421 ccatcccagt gcgcgtatcc aggccaaggg caggtgcgcc agcagcctca tgaaaagaat
     3481 gcccagttcg ctcatggcct gctgctgcca ccggccggcg ccttgtagcg cgcatagccc
     3541 cagaggtact ggtgcgggcg ctcgcggatg aggtgctcca tctgggcgtt gacgcgcgcc
     3601 gccgcatctg cggcttcgct cggaagctcg cccggccacg gccgcaagcg gatgcgatag
     3661 ccgcgccccc agggcaggcg ctcgccccat gccagcagca cggtggcgcc ggtctgctgc
     3721 gcgagtcgcg ccggcaaggt catggtgtag gccgcgcggc cgaaaaatgg cgcccagaca
     3781 cccaggcctt caggcggcac ctggtccggc aagaggccca cggcctcgcc ggccttcaag
     3841 gccttcagca actgtttcac gccacccagc gtggtcggcg ccgtggcgag attggcacgg
     3901 tcgcgcgcag tgtcgaccag gtcacgcaac cagggcttgc gcgccggacg gtacagcacg
     3961 gtgatgcggc cgtagcgcat ggcgtagccc tgggccgtga cctcgaagca acccaggtgc
     4021 ggcgtcagga aaacgatgcc acggccgtgc gcccgcgcca tgtcgaccag ctcggcgcct
     4081 tcccaatgga tggctaccga gcgtccgaac cagagccggg gcgtttcggc caccagcttg
     4141 ccggcttccg cgatggccga tcgaacctgc gcgagcgtgt agccggcctg ttgcgcgttg
     4201 tcggtgaacc ggcggcggta ggtggacgat gccaggaaag acacccagcc cagggccgcg
     4261 ccgaccgcgt gcaggacagg cagcggcagc agcgcgaaca cgcggaagag ggttttcaca
     4321 agcggtttag aatgctcatg tcgccgagtt atgaactact tgcagggcga cattaaaaaa
     4381 gttctgctaa agcgttcgcc ccaagctccg gctcaaggca acgcgccttc agcaacccgt
     4441 ggagtttatt caaatggcga acgactttct cttcacttcc gaatcggtat ccgaaggcca
     4501 tcccgacaag gtgtccgacc agatctccga cgcgatcctc gatgccgtct tcgcccagga
     4561 cccgcgctcg cgcgtggccg ccgagacgct gtgcaacacc ggcctggtgg tgctggccgg
     4621 cgaaatcacc accaacgccc atgtcgatta catccaggtg gcgcgcgaca ccatcacgcg
     4681 catcggctac gacaacaccg aatacggcat cgactacaag ggttgtgcgg tgctggtcgc
     4741 ctacgacaag cagagcccgg acatcgccca gggcgtggat cgcgcctccg acgactatct
     4801 gaaccagggc gcgggcgacc agggcctgat gttcggctac gcctgctccg agaccgacac
     4861 gctgatgccg ttgccgatct acctgtcgca ccgcatcgtc gagcgccagg cgcaattgcg
     4921 ccacgacggt cgcctgccct ggctgcgccc ggacgcgaag tcgcaggtca cgatcaagta
     4981 tgtcgacggc aagccgcaca gcatcgacac cgtgctgctg tccacccagc atgccccgga
     5041 aatgacgctg gagcagatcc gcgaagccgc catcgaggag atcatcaagc cggtgttgcc
     5101 ggccgaactg atcaagggcg acatcaaatt cctggtgaac ccgaccggcc gtttcgtcat
     5161 cggcggcccg cagggcgact gcggcctgac cggccgcaag atcatcgtcg acacctacgg
     5221 cggcgcggcc ccacacggcg gcggcgcgtt ctccggcaag gatccctcca aggtggaccg
     5281 ctcggcagcc tatgccggcc gctacgtggc gaagaacatc gtcgccgcgg gcctggccga
     5341 caagtgcctg gtgcaggttt cctacgcgat cggcgtggcg cagccgacca gcatcatggt
     5401 cagcaccttc ggtaccggcc acatctccga cgagaagatc accgaactgg tgaagaagca
     5461 cttcgacctg cgtcccaagg gcatcgtgca gatgctggac ctgctgcgcc cgatctacca
     5521 gaataccgcc gcctacggcc actttggtcg cgaagagccc ggcttcactt gggagaacac
     5581 ggacaaggcg gcggcgctcc gagccgacgc cggtttgtaa gcagcgctta caaaccgcac
     5641 gccataggcg tgcttgtcct tattccggcg caggctaaca tcgcttgcga tgttaaggtg
     5701 cgtagcactg gccgaagcca aaatactgac aaagcagccg cgctccgcgc ggatgccgga
     5761 ctgtaagtcc gccgcgtagc ggtttgtcat tgtttcggcg caggctcata tccgcgtagc
     5821 ggatgtgaag ggggcgcaag cccccggccg aagccaaaac attgacagta aggtgcttgc
     5881 accttattgc ggtgcagact gacctttagg tcatgtcgga gccacaaggc aacaaatcga
     5941 agccgccccc gatgaaactc gggggcggct ttttttcata tagaatcccg agcacttcca
     6001 tcgggcaggg gctgccgtgc tgaaacgtat tccggttgaa caattgagat tgggcatgtt
     6061 cgtccatgag ttttgcggtt cgtggatgga gcatccgttc tggcgcggcg ccttcctgct
     6121 ggacgacccg aaagacctgg aaaccatcct gtccaccggc ctcaaggagg tgtggataga
     6181 tacctccagg gggcaggatg tcgaaggcgg cgcgacccgg gaggcggtgg cagccgaggt
     6241 cgaggccgcg ctggtgcagg tggagcctgg caagatggtg ccgcgcaccg cggttgcaca
     6301 ggaggcagta cgggccgtga agatctgcgc ccagtccaag caggccgtgg cttcgatgtt
     6361 ccacgaggcg cgcatgggca aggcgttgga tgtcggcgat gcgctgccca tcgtcgagga
     6421 aatctcgacc tcggtgatgc gcaacccggg cgcgctgatc agcctggcgc ggctgaagaa
     6481 caaggacgaa tacacctata tgcattcggt cgcggtatgc gcgctgatgg tgtcgctggc
     6541 gcggacgctg ggcttcaacg atcagcagac gcggcaggcg gggctggccg gactgctgca
     6601 cgatgtcggc aagatgatga tcccgctgtc catactcgac aagccgggca agctgaccga
     6661 ggcggaattc gagacgatga agagccatcc ggtcgaaggc caccggatgc tgctgggcgg
     6721 cacgggaatc ggcgaagtcg cgctcgatgt ctgtctgcac catcacgaga agatggacgg
     6781 cagcggctac ccgcacggac tgggcggcgg ggagatttcc cagtatgcaa ggatgggggc
     6841 ggtgtgcgat gtgtatgacg cgatcacctc caatcgcccg tacaagaacg gctgggagcc
     6901 ggccgagtcg atacgcaaga tggccgagtg gagcaagggc catttcgatc cgcggatatt
     6961 cgaggccttc gtgcgcagca tcggcatcta ccccatcggc tcgctggtca ggctggcgtc
     7021 ggatcatctg ggggtggtcg tcgaacagac ggaacagtcg ctgctgaccc cgatcgtcat
     7081 ggtgttcttc tcgatcaagt cggacgccag gatagcgccg gaattgctga atttgtcgaa
     7141 accgggctgc aaggacagga tcgtttccca cgaagacccg gtcaagtggg acatcaagga
     7201 cacgcaggcg ctgtggagcg gcatggacgt cgcgccctgg tagtccgccg cgcgcccggt
     7261 tttgcttttg cgctataatc cgccccctcc gaggggtgct gcagcgggtt gagcgtcatt
     7321 tccccgccag gctcggatgt ccaaacggca cccattcatt aacctttatg aatggagctt
     7381 gctcatgaat gctgttgctt tcaacgatta cgtcatcgcc gacatcaaac tggccgattg
     7441 gggccgcaag gaaatcgcca ttgccgagca cgagatgccc ggcctgatgg ccatccgcga
     7501 agaattcgcc aagacccagc cgctgaaggg cgcgcgcatc accggttcgc tgcacatgac
     7561 catccagacc gccgtgctga tcgagacgct gaccgcgctc ggcgcggaag tgcgctgggc
     7621 ttcgtgcaac atcttctcca cccaggatca cgctgcggct gccatcgccg ccgacggcat
     7681 cccggtgttc gcggtcaagg gcgagacgct ggccgaatac tgggactata cccaccgcat
     7741 cttcgaatgg gcggacggcg gctattcgaa catgatcctc gacgacggcg gcgacgccac
     7801 gctgctgctg catctgggcg cgcgcgccga gactgacgct tcgctgatct ccaagccggg
     7861 ttcggaagaa gagacctgct tgttcgccgc aatcaaggcc aagctggcga ccgacccgac
     7921 ctggtactcc acccgcctcg cccagatcaa gggcgtgacc gaggagacca ccaccggcgt
     7981 gcatcgtctg tatcagatgc atcagcgcgg cg
//
LOCUS       DASWIY010000307         5227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_584161, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000307 DASWIY010000000
VERSION     DASWIY010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584161"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VGA59_11180"
     CDS             complement(<1..221)
                     /locus_tag="VGA59_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor MviN"
                     /protein_id="HEX9720284.1"
                     /translation="MFLKAGALSLALLLASRVLGLVRESAQAAAFGTTGLADVVVLML
                     TLPDWLASVLASGALAYVLVPAWAGQARA"
     gene            311..1849
                     /gene="lysS"
                     /locus_tag="VGA59_11185"
     CDS             311..1849
                     /gene="lysS"
                     /locus_tag="VGA59_11185"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX9720285.1"
                     /translation="MSDKNPPAPPTPASDENQLIAERREKLKALREAERQGKGIAFPN
                     DFKPGHHAADLLAHHGTKTPELLAEQGLQVSIAGRMMLKRVMGKASFATIQDATGRFQ
                     IYVTRDDVGEDTYAAFKHWDLGDIIGAEGKLFKTRTGELSLHASVIRLLTKNLRPMPD
                     KFHGVADQEVKYRQRYVDLMMDPATRDRFVARSKAVGSIREFMVSHGFLEVETPMLHP
                     IPGGANAKPFVTHHNALDQQMFLRIAPELYLKRLIVGGFERVFEINRNFRNEGISVRH
                     NPEFTMMEFYAAYWNYQDLMDFTEALMRDAAEKAVGTLQLSYSGRPVDLAKPFERLTI
                     REAILLHTEAGDNADDRQWLVNALRKLGLNEEKHKLSARSLASLQVMYFEETVEEKLW
                     HPTFIMEHPTEISPLARAHAARPDVTERFELYITGREFGNGFSELNDAEDQAARFLAQ
                     VNAKDAGDDEAMFYDQDFVRALEYGMPPTGGCGIGIDRLMMLLTDSPSIRDVILFPAL
                     RHEA"
     gene            complement(1885..2967)
                     /gene="nagZ"
                     /locus_tag="VGA59_11190"
     CDS             complement(1885..2967)
                     /gene="nagZ"
                     /locus_tag="VGA59_11190"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX9720286.1"
                     /translation="MSEHAPVILDIAGTALSEVDRRRLAHPLAGGIILFGRNWQDRAQ
                     LAALCADIKSVRQDLLICVDHEGGRVQRFRTGGFTHLPPMRALGQLWMNGGGRGDGTG
                     AMAATDAATAAGYVLGAELRACGVDFSFTPVLDLDWGASGVIGDRSFHRDPRVAALLA
                     KSLMHGLLRAGMANCGKHFPGHGFVQADSHTDVPVDRRSLKAILADDAAPYRWLSTSL
                     ASVMPAHVIYPKVDPRPAGFSSRWLNDILRRQLGFGGAIFSDDLSMAGARLIDGREVS
                     FAQAAIAALNAGCDMVLLCNQCVRDPPAVDALLDGLAEAQLKGWWRPGGASEQRRLAL
                     LPGAPALPWDALMAHPDYIHALGLLP"
     gene            complement(2964..3365)
                     /gene="acpS"
                     /locus_tag="VGA59_11195"
     CDS             complement(2964..3365)
                     /gene="acpS"
                     /locus_tag="VGA59_11195"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX9720287.1"
                     /translation="MIHGIGTDVCDLRRIRASLQRHGERFARKILNDAEMAVWKSRSV
                     RWPERGIRFLATRFSAKEAFSKAVGLGLRLPMSWQLCEIANLPSGKPVIVLHGALKDW
                     FEAQGLAAHVSVTDESDYAASFVVVEKKAAP"
     gene            complement(3362..4132)
                     /locus_tag="VGA59_11200"
     CDS             complement(3362..4132)
                     /locus_tag="VGA59_11200"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX9720288.1"
                     /translation="MTSPTRTALSVNLNKVALVRNTRHLGIPSVERAAEICLRAGANG
                     ITVHPRPDARHIRAADVRALHGLLRSWPGAEFNIEGNPFHNLMDFVRELRPHQCTFVP
                     DSEGQFTSDHGWNLPADRERLVPVIAQAKALGVRVSLFMDPLPHAMAAAKAVGADRVE
                     LYTESYANAWGTARQDEVLRSFVEAARAAQAVGLEVNAGHDLNRDNLTDFLCAVPAVR
                     EVSIGHALISDALELGYAATVKAYLDCIARANPDSGGA"
     gene            complement(4129..4881)
                     /gene="recO"
                     /locus_tag="VGA59_11205"
     CDS             complement(4129..4881)
                     /gene="recO"
                     /locus_tag="VGA59_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX9720289.1"
                     /translation="MAVKRVSDEPAFVLHRYDWSESSLILEVFTREYGRVALVAKGAK
                     KPSSSFRPILLPLQPLRIAFGGDAEIRTLKGAEWVGGQVMPTGDALLSGYYLNELVLR
                     LLARDDPHPGLFDAYAPAVQVLASEHGEALQPALRAFELVLLREIGLLPVLDLQTLAL
                     APLAAQERYSLVPEGGLVQASHGDGRASLSGSQWRALQRALDDAAPFSATLRECANVA
                     PELKPQLRTLLNYHCGVSTLRTRQLMIDLQAL"
     gene            complement(4895..>5227)
                     /locus_tag="VGA59_11210"
     CDS             complement(4895..>5227)
                     /locus_tag="VGA59_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX9720290.1"
                     /translation="LAAETVREKLFRFTGDELPYTSTVVVDKFEEEPSAKHGRMVRIA
                     ATIVVEREGHKAMVIGDKGERLKRIGTEARQELEKLMDCKVFLEIWVKVRTGWADDEA
                     RVRSFGYE"
BASE COUNT          913 a         1798 c         1700 g          816 t
ORIGIN      
        1 tccgctctcg cctgtccggc ccaggccggc accagcacat acgccagcgc gccgctcgcc
       61 agcacactgg cgagccaatc cggcaacgtg agcatcagca ccaccacgtc ggcgaggccc
      121 gtcgtgccga aggcggctgc ctgcgccgac tcgcgcacca gtcccagcac ccggctggcc
      181 agcagcaggg ccagggacag agcgccggct ttcaaaaaca tggcatgaat tatcgagggc
      241 ggcgggccgc agccgacaaa aaactaaaat ccatggtttg gccccgcctc cgccctactc
      301 cttccctttc atgtccgaca agaacccccc tgctccgccc acgccggcat ccgatgaaaa
      361 ccagctgatc gccgagcgcc gcgagaagct caaggccctg cgtgaggccg agcgccaggg
      421 caaggggatc gcgtttccga acgatttcaa acccggccac catgcggcgg acctgctggc
      481 tcatcacggc acgaaaacac ccgaacttct cgccgaacaa ggtttacagg tcagcatcgc
      541 cggccgcatg atgctcaagc gcgtgatggg caaggcgagc ttcgcgacca tccaggatgc
      601 gaccggccgc ttccagatct atgtgacgcg cgacgacgta ggcgaggata cctacgccgc
      661 cttcaagcac tgggacctgg gcgacatcat cggcgccgag ggcaagctgt tcaagacgcg
      721 caccggcgag ctgtcgctgc atgcaagcgt catccgcctg ctgaccaaga acctgcggcc
      781 catgcccgac aagttccatg gcgtggcgga ccaggaagtg aagtaccgcc agcgctacgt
      841 ggacctgatg atggatccgg ccacgcgtga ccgcttcgtg gcgcgcagca aggccgtggg
      901 cagtatccgt gagttcatgg tgtcgcacgg tttcctggaa gtcgaaacgc cgatgctgca
      961 cccgattccg ggcggcgcca acgccaagcc cttcgtcacg caccacaacg ccctggacca
     1021 gcagatgttc ctgcggatcg cgcccgagct gtacctgaag cgactgatcg tcgggggctt
     1081 cgagcgggtg ttcgagatca accgcaactt ccgaaacgaa ggcatttcgg ttcgccacaa
     1141 tcccgagttc acgatgatgg agttctatgc ggcgtactgg aattaccagg acctgatgga
     1201 cttcaccgaa gcattgatgc gcgacgccgc cgaaaaagcc gtgggcacgt tgcagctgag
     1261 ctacagcggc cggcccgtgg acctggcaaa gccgttcgaa cggctcacca tccgcgaagc
     1321 gatcctcctg cacaccgagg ccggcgacaa cgcggacgac cggcagtggc tggtcaacgc
     1381 gctgcgcaag ctgggcctga acgaggaaaa gcacaagctg tccgcccggt cgctggccag
     1441 cctgcaggtg atgtacttcg aagaaacggt ggaagaaaag ctctggcacc cgaccttcat
     1501 catggagcac ccgaccgaga tttcaccctt ggcgcgtgcc cacgccgcgc ggcccgatgt
     1561 cacggaacgc ttcgagctct atatcaccgg ccgcgagttc ggcaacgggt tttccgagct
     1621 caatgatgcc gaggaccagg cggcccgctt cctggcccag gtgaacgcca aggatgcggg
     1681 cgacgacgaa gccatgttct acgaccagga tttcgtacgt gcgctcgaat acgggatgcc
     1741 gcccacgggc ggctgcggca tcggcatcga ccggctgatg atgctgctca ccgacagccc
     1801 cagcatccgc gacgtgatcc tgttccccgc tttaaggcac gaagcctagg accggcgttc
     1861 cggcggcgcc ggttcacggt ttgactacgg gagcagcccg agcgcgtgga tgtagtcagg
     1921 atgcgccatc agggcgtccc acggcaaggc gggcgcgccc ggcagcagcg ccaggcgccg
     1981 ctgctcgctg gccccgcccg gccgccacca gcccttgagc tgcgcctcgg ccaggccatc
     2041 gagtagcgcg tccacggccg ggggatcccg gacgcactgg ttgcacagca gcaccatgtc
     2101 gcaacccgcg ttcagcgccg caatggcggc ttgcgcgaag ctcacctcgc gtccgtcgat
     2161 cagccgcgcg cccgccatgc tgaggtcgtc gctgaagatg gccccgccga agcccagctg
     2221 ccggcgcagg atgtcgttca gccagcggct ggaaaagccc gccggccgcg gatcgacttt
     2281 cgggtagatg acgtgcgccg gcatcacact ggccagggag gtgctgagcc atcggtaggg
     2341 agccgcatcg tccgcgagga tggccttgag gctgcgccgg tcgacgggca cgtcggtgtg
     2401 cgagtcggcc tgcacgaagc catgccccgg aaagtgcttg ccgcagttgg ccatgcccgc
     2461 gcgcagcagg ccgtgcatga ggctcttggc cagcagcgct gcgacgcgcg ggtcgcgatg
     2521 gaaggagcgg tcgccgatca cgccgctcgc accccaatcc aggtcgagca cgggggtgaa
     2581 gctgaagtcc accccgcacg cgcgcaattc ggcgcccagc acatagcccg cggccgtggc
     2641 cgcgtcggtg gcggccatgg cgcccgtgcc atcgccccgg ccaccgccgt tcatccacag
     2701 ctggcccagg gcccgcatcg gcggcagatg ggtgaatccg ccggtgcgaa agcgctgcac
     2761 cctcccgccc tcatggtcaa cgcagatcag caggtcctgc cgcacggact tgatgtcggc
     2821 gcacaaggcc gccagctggg cgcggtcctg ccagttgcgc ccgaaaagaa tgattccacc
     2881 cgcgagcgga tgcgccagcc ggcggcggtc cacctcgctc aaggcggtac cggctatgtc
     2941 caggatcaca ggggcgtgtt cgctcatggc gcggctttct tctcgaccac gacaaagctc
     3001 gcggcgtaat cgctttcatc ggtgacgctg acatgggccg caagcccctg cgcctcgaac
     3061 cagtccttga gcgcgccatg caggacgatg accggcttgc cgctgggcag gttggctatt
     3121 tcgcacagct gccagctcat gggcagccgc aggcccagtc cgaccgcctt gctgaacgcc
     3181 tccttggccg aaaagcgcgt ggccagaaag cggatgccgc gctccggcca gcgtacgctg
     3241 cgcgatttcc agaccgccat ttcagcgtcg ttcaggatct tgcgcgcaaa gcgctcgcca
     3301 tgccgctgca gcgacgcgcg gatgcggcgc aggtcgcaga catcggtccc tatgccgtgg
     3361 atcatgcgcc gccgctgtcg ggattggccc gggcgatgca gtccaggtag gccttcacgg
     3421 tggcggcata acccagttcc agcgcgtccg agatcagggc atggccgatc gatacctccc
     3481 gcacggccgg cacggcgcac aggaaatccg taaggttgtc ccggttgagg tcgtgccctg
     3541 cgttgacttc caggcccacg gcctgggcgg ctcgcgcggc ttcgacgaaa gagcgcaaca
     3601 cctcgtcctg ccgggccgtg ccccaggcat tcgcgtaact ctcggtgtaa agctccaccc
     3661 ggtccgcccc gacggccttg gccgccgcca tcgcgtgcgg caacgggtcc atgaacaggc
     3721 tgacccgcac gcccagcgcc ttggcctgcg cgatcaccgg caccagccgc tcgcggtcgg
     3781 ccggcaaatt ccagccatgg tcgctggtga actggccctc gctgtcgggc acgaaagtgc
     3841 attgatgggg ccgcagttcg cgcacgaaat ccatcaggtt gtggaatgga ttgccctcga
     3901 tgttgaactc ggcgccgggc caggatcgca gcagcccgtg cagggcgcgc acatccgccg
     3961 cgcggatatg ccgcgcatcc ggtcgcggat gcaccgtgat gccgttggcc ccggcccgca
     4021 ggcagatttc ggccgcgcgc tcgacgctcg ggatgccaag gtggcgcgtg ttgcggacca
     4081 gggcgacctt gttgaggttc accgagaggg cggttcgggt tggcgaagtc atagggcttg
     4141 caggtcgatc atcaactggc gggtgcgcag cgtcgacaca ccgcaatggt agttcaggag
     4201 cgtgcgcagc tgcggcttga gttccggcgc gacgttcgcg cattcgcgca acgtggcgga
     4261 aaacggcgcg gcgtcgtcca gcgcgcgctg caaggcccgc cactggctgc cgctcaggct
     4321 ggcgcggcca tcgccgtgcg acgcctgaac gagcccgccc tcgggtacca ggctgtagcg
     4381 ttcctgggcg gccaggggcg cgagtgccag cgtttgaagg tccagcacgg gcagcagccc
     4441 gatctcgcgc agcagcacca gttcgaacgc gcgcaacgcg ggctggagcg cctcgccgtg
     4501 ctcggaggcc agcacctgga cggccggggc ataggcgtcg aacaggccgg gatgcggatc
     4561 gtcgcgcgcc agcagcctca gcaccagttc gttgaggtaa tagcccgaca gcagggcgtc
     4621 cccggtgggc atcacctgcc ctcccaccca ttcggcgccc ttgagcgtgc gaatctccgc
     4681 gtcaccgcca aaggcgatgc gcagtggctg caacggcagc agaatcggcc ggaagctgga
     4741 gctcggcttc ttggctccct tggcaaccag cgccacgcgg ccgtactcgc gggtgaagac
     4801 ttcgaggatc aggctggatt cgctccagtc gtaacggtgc aggacgaagg cgggctcgtc
     4861 ggagacgcgc ttgacggcca tggggctggg ccggctactc gtagccgaag ctgcgcacgc
     4921 gcgcctcgtc atccgcccag ccggtgcgca ccttcaccca gatttccagg aacaccttgc
     4981 aatccatcag cttttccagc tcctggcgcg cctcggtgcc gatgcgcttg agccgctcgc
     5041 ccttgtcgcc gatcaccatg gccttgtggc cctcgcgctc gaccacgatt gtcgccgcga
     5101 tcctgaccat gcggccgtgt ttcgcactgg gctcttcctc gaacttgtcg accaccacgg
     5161 tggaggtgta gggcagctcg tcgccggtga accggaacag cttttcgcgc acggtctcgg
     5221 cggccag
//
LOCUS       DASWIY010000308        18354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1308433, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000308 DASWIY010000000
VERSION     DASWIY010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 18354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18354
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308433"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1253)
                     /locus_tag="VGA59_11215"
     CDS             complement(51..1253)
                     /locus_tag="VGA59_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9720291.1"
                     /translation="MSKRTYQWLGLALFVLAIAFSVMKALSARKAQQEAVAQASIPKV
                     QTVVELADSDVVKAETRELAQGLPISGSLKAVNSALVKARAAGELQGLALREGDAVKA
                     GQVIARVDATEYNARVKQAQEQADAAKAQIDSAQRQWDNNKALVDQGFISKTALDTSW
                     NNFAAAQATHKAALAAVEMARKTLDDTVLRAPISGVVAQRLAQPGERVGIEARVIEIV
                     DLSRLELEATLSPADSMDLRVGQEALLQIEGSNRPVRARVARINPSAQAGSRSVLAYL
                     VIADASGLRQGLFAQGTLGTGRTEALAVPLSAVRTDKPAPYVQAVENNQIVHKPVELG
                     ARGESGKEVIVAVKGLAAGTVVVRGNIGLLREGTAVRFTAGGPHPSPLPQAGEGATTR
                     APAKAPSP"
     gene            complement(1250..1954)
                     /locus_tag="VGA59_11220"
     CDS             complement(1250..1954)
                     /locus_tag="VGA59_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9720292.1"
                     /translation="MSLSKLIGMPQEEVQAKRERRKEARPGELLDAALDLFVEKGFAA
                     TRAEEVAARAGVSKGTLFLYFPSKEDLFKAAVRENISGRFPGWNKEFEEFQGSTPDML
                     RHFMRMWWERIGATKASGLTKLIISEARNFPDIAAFYQQEVIQPAHLLIRKMLQRGVD
                     RGEFEVPDMDYALFTVTAPMVFLMMMKHSMGACVPRDFPLDPERYVASQVETLLRGLC
                     VRPEDKGGKKDGRANP"
     gene            2277..2819
                     /gene="msrA"
                     /locus_tag="VGA59_11225"
     CDS             2277..2819
                     /gene="msrA"
                     /locus_tag="VGA59_11225"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX9720293.1"
                     /translation="MSTSSQTIVLGGGCFWCTEAVYVKVRGITDVESGYSNGHVPQPT
                     YEQVCTGTTGHNEVVKLTYDPSQISVRQVLEIFFVIHDPTTLNRQGADSGTQYRSGIY
                     HTTPEQKDVADDMIRQMSQDKLFGKPIVTEVLPLANYWPAEEYHQDFFEKNPYQGYCM
                     AVAGPKVAKFRKTFAELVKQ"
     gene            2833..3501
                     /locus_tag="VGA59_11230"
     CDS             2833..3501
                     /locus_tag="VGA59_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9720294.1"
                     /translation="MVETRGLRYQYPGGPALSFDDLDVPQGATLLLQGVSGAGKSTWL
                     ALAAGLLTPSAGEAVIAGQRLSALSRAARDAWRGRHLGFLPQKLHLSEALTVERNMAL
                     AFYAAGVPEIRGTVQTALNLLGVGELAGRRPSQLSGGQAQRVALARTILMEPRIILAD
                     EPTASLDDEAASTGLKLLENCAFQCSATLVIATHDRRVQEALPAAMVYRIGHRAAQGS
                     VAAA"
     gene            3498..4700
                     /locus_tag="VGA59_11235"
     CDS             3498..4700
                     /locus_tag="VGA59_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9720295.1"
                     /translation="MKTIALAFRYLWSRPLQALLNLLLLTLGLASITFVLLASEQIDR
                     AFQRDLAGIDLVVGAKGSPMQLILAGVFHIDVPPGNISLADVEELRRQPQVAKLIPLS
                     LGDAFRGFRIVGTNADYLSHYRMAFAQGQPWAQSMEAVLGAQAAQATAMKTGDSFIGS
                     HGLGGGGHGHGEHPYKITGVLAPCGCVVDRLILTPTESVWQVHEKAMAVDDEDRKALE
                     AEREITIALITYNSPLAAVTFPRYVNSRTDMQAAAPAVEVSRLLRMLGVGSEVLRGVG
                     AVLLLTAALSVFVALWNAVRERRADLAMLRMLGATPAKVAALLVCEALWLAVLASVLG
                     LAAGHGLTALVGWLLEGQRSLPMSGRLWVPAEAWIPAAAVGVAALAALIPTVSAYRVD
                     VAHLLNTR"
     gene            4720..4941
                     /locus_tag="VGA59_11240"
     CDS             4720..4941
                     /locus_tag="VGA59_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720296.1"
                     /translation="MFLAAWLPLLALAAESPEEIKKDAQRHRAMAVAHEAAAKCLESG
                     KNPDQCQKDLQAACKGLAIGKYCGMKHAH"
     gene            5010..5501
                     /locus_tag="VGA59_11245"
     CDS             5010..5501
                     /locus_tag="VGA59_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HEX9720297.1"
                     /translation="MKRIVLVLAALFAASAGAQLSPPVPLGTGPGVHSPNSPFAPLPA
                     RDDVVPWSLLTAVKTKTEKNKVLPVFDKPQLALNAKTQRVQGFMMPLEPGEKQTHFLL
                     SSIPLTCAFCVPGGPESMVEVKTRTPVKYTLDPVTVEGMFAVLNDDPYGLYYRITDAA
                     SIR"
     gene            complement(5544..5930)
                     /locus_tag="VGA59_11250"
     CDS             complement(5544..5930)
                     /locus_tag="VGA59_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEX9720298.1"
                     /translation="MKRWVWLHREIILAVHEHQLAEHGGAAGVRDQGLLESALARPEN
                     LAAYGKPDIADLAAAYGYGISRNHAFIDGNKRTGFVATELFLELNRHALAADDVDCVV
                     TMLAVASGDITEAEFAAWIRDHAAKR"
     gene            complement(5927..6151)
                     /locus_tag="VGA59_11255"
     CDS             complement(5927..6151)
                     /locus_tag="VGA59_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9720299.1"
                     /translation="MYTLKLTQVGNSVGAIFPKEVLARLNLEKGDELYLTDSPDGLRL
                     TVHNPEFEVQMKAARDVMKKRRAVLRELAK"
     gene            complement(6305..6952)
                     /gene="pdxH"
                     /locus_tag="VGA59_11260"
     CDS             complement(6305..6952)
                     /gene="pdxH"
                     /locus_tag="VGA59_11260"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020684118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX9720300.1"
                     /translation="MSTTSSRLAELRKSYERAELSEEASYADPLKQFDQWLAEAISAE
                     IPEPNAMTLATVAGDLRPSTRIVLIKGFDERGIVWYTNYESRKGQELGGNPYAALQFH
                     WVELERVVRIEGRVEKTSAEESDTYYATRPLDSRIGAWASPQSQVIASRTLLVANAAK
                     FGAQYLLNPPRPSHWGGYRLVPDRWEFWQGRKSRLHDRLRYRLEGGNWVRERLAP"
     gene            7036..8016
                     /locus_tag="VGA59_11265"
     CDS             7036..8016
                     /locus_tag="VGA59_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX9720301.1"
                     /translation="MESLDLRVLGDALAWRRAGHAVTLVTVVETWGSAPRPPGALLAV
                     RDDGVVSGSVSGGCVEDDLIARVKAGERNAAPSMIAYGVSKDEAARFGLPCGGTLRLV
                     QEPLRETGWVEELLARTAAHELVARTLQLASGQVTLAAASRDQSMRFDGVELTTLFGP
                     KWRLLLVGAGQLSQAVAQMAQMLDFEVMVCDPREEYSNTFAVTGARHVPGMPDDVVRD
                     MRPDGHTAIVALTHDPKLDDMALLEALRSDAFYVGALGSSRNQAARKARLAEHFGLTG
                     EELARLHGPVGLRIGAKTPAEIAVSILGQIVECKNPAAQALRPAAGCAVT"
     gene            8013..8552
                     /locus_tag="VGA59_11270"
     CDS             8013..8552
                     /locus_tag="VGA59_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX9720302.1"
                     /translation="MNQPVVLVLASGRGERFVASGGSGSKLRALLGGKPVLERTLDAV
                     RASGLPWHLEDAGHEGMGDSIAAAVRATQGAPGWLVLPGDLPLVRPQTLQAVAAALAD
                     HVVVLAHYRGQRGHPVGFAASCRDALLLLQGSQGAALVVRAQATVKPVAQLELDDPGI
                     VTDIDTLQDLVNAERLLAG"
     gene            complement(8868..9260)
                     /locus_tag="VGA59_11275"
     CDS             complement(8868..9260)
                     /locus_tag="VGA59_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX9720303.1"
                     /translation="MRFVQSGDVVRVSGEVRGLKPGAEHGFHVHEKGDCSSPDGQQRR
                     WPLQSRWQAPRRHGQGVHHAGDLPGLRADANGVAAFSFESRTVKVGSGPTDVVGKGLI
                     VHRDPDDFTTQPTGNAGPRLACAVIARG"
     gene            complement(9483..10031)
                     /locus_tag="VGA59_11280"
     CDS             complement(9483..10031)
                     /locus_tag="VGA59_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEX9720304.1"
                     /translation="MLERVLQEWGGHEDLWIFGYGSLIWRPDFEFAERRPARVHGWHR
                     ALKMWSRINRGTPERPGLVFGMLSGGCCQGMVFRIPRQAGQQVLTHLWSREMIMGVYD
                     PRWLTSHTSHGPVTALAFTLSRKSPSHTGALTEAQYRLIFSEASGIYGSTLDYAHRTL
                     EELRRHNIRDRGLERLLKLMQR"
     gene            10281..12098
                     /gene="edd"
                     /locus_tag="VGA59_11285"
     CDS             10281..12098
                     /gene="edd"
                     /locus_tag="VGA59_11285"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEX9720305.1"
                     /translation="MKPHPVLAAVTERIRERSAPRRAAYLRQIDEAAGRDRGADRMGC
                     ANVAHAFAGLPPNDKFRVVAEKAPNIGIVNAYNDMLSAHAPLGRFPDLIKEEARKHGA
                     TAQVAGGVPAMCDGVTQGTAGMELSLFSRDVIAMATAVSLSHDVFDAALMLGICDKIV
                     PGLLIGALHFGHLPMVFVPGGPMPSGLSNSEKAKVREQAAQGLVGREALLDAESRAYH
                     APGTCTFYGTANSNQMLMEAMGLHVPGTAFVNPGNELRDELTREAVRTVLDILKGRRF
                     APIGRLVDERAVANAMAALLATGGSTNHLIHWIAVARSAGITIDWDDFAQLSAVVPLL
                     ARVYPNGSADVNQFQAAGGPGFVIRELLDAGLMHEDVLTVRSGGIREYTGEPRLADAG
                     LAWSPIGPSRDTDIVRPAADPFSATGGLKLLAGNLGRSVIKVSAVPADRHLIEAPARI
                     FDAQEELQRAFNAGELDRDVVCVVRWQGPQANGMPELHKLTPPLAVLQGRGFRVALVT
                     DGRMSGASGRVPAAIHVSPEAAAGGPLARLRDGDAVRLDADAGVLQALVPAHEWAARE
                     NAAMPEALSSANGLGIGRELFAAFRRNALPAEEGACTWL"
     gene            12089..12754
                     /gene="eda"
                     /locus_tag="VGA59_11290"
     CDS             12089..12754
                     /gene="eda"
                     /locus_tag="VGA59_11290"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX9720306.1"
                     /translation="MAVGNEQFAAGRLTSLQVMTDAPVIPVIVLTDAAHAVPLARALV
                     AGGIRMLEVTLRTPAALACIEAIARDVPEAMTGAGTVRSAADAQAAAMAGARFAVSPG
                     YTHATGRACHDLELPLLPGVATGSEIMAAQADGYTALKFFPAMPAGGVAMLKAWQGPF
                     GDVTFCPTGGITAANAPDFLALANVVCVGGSWLTPADAVASGDWARITQLAREAVALQ
                     PSR"
     gene            complement(12770..13165)
                     /gene="gloA"
                     /locus_tag="VGA59_11295"
     CDS             complement(12770..13165)
                     /gene="gloA"
                     /locus_tag="VGA59_11295"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEX9720307.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTHVLGMTLLRTTERPEQNYSLAFV
                     GYGSNPEHAEIELTYNYGVDRYDLGTGYGHIALGVPDVYVACEKIRAGGGNITREPGP
                     VKGGTTVIAFVTDPDGYKIELIQRSTESV"
     gene            13250..13723
                     /locus_tag="VGA59_11300"
     CDS             13250..13723
                     /locus_tag="VGA59_11300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9720308.1"
                     /translation="MILLPSVRLATPHDARGIAEMSREYIEHGLGWTWTRERVLKAID
                     ERSTNVAVVREDHRLLGFGIMQYGDQRAHLSLLGVQASQRQRGLATLLLSWLEKSAAV
                     AGIERIQLEARSDNAIALAFYRGQGYRQTGVITGYYRGVLDAVRLEKKLGPTAES"
     gene            13870..15075
                     /locus_tag="VGA59_11305"
     CDS             13870..15075
                     /locus_tag="VGA59_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9720309.1"
                     /translation="MALSCAHRLHRLLAVAGFTCLLSACGQGGSAQSAAPAPPAEVGV
                     VTVAPGDVGLVTELPGRLEASRVAQVRARAAGIVQRRLFREGSDVRAGQALFTIDPAP
                     YAAAHASAQAALARAQANLGQAAALAERYKPLVEANAISKQEYANAVAARKQGEAEVA
                     AARAAVQTAQINLGHASVTAPISGRIGRALVTEGALVGQGEATPLAVIQQINPMYVNF
                     TQPATEVLNLRRAFEQGRLKRAIGAEGALVRVVLEDGSEHPNPGRLLFSDLSVDPTSG
                     QITLRAEVPNPGGALLPGLYVRVRLEQAKAGNAVLLPQQAVTRSAQGDTVMVVASDGQ
                     VSPRPVKVGGSQAGQWVILEGLKPGEQVIVDGFQKLRPGAAVKPVPWRAGPAAAATPA
                     SAPAAAAKS"
     gene            15088..18312
                     /locus_tag="VGA59_11310"
     CDS             15088..18312
                     /locus_tag="VGA59_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEX9720310.1"
                     /translation="MAKFFIDRPIFAWVIALFIIVLGGVAITQLPIAQYPPVAPPSIV
                     ISTNYPGASARTLEESVISVIEQEMNGSPGLIYMESVSQANGSGQITLSVQPGTPPDL
                     AQVDVQNRLSRASPRLPQAVTQQGVRVDKARSNFLLFTILSSDDPAFTPVALGDYASR
                     SVLPEIQRIPGVGQARLFGTEQAMRIWIDPAKLLGFNLSSAEVNAAIRAQNAQVSSGS
                     IGDLPNIAGQGIAATVVVNGQLASVEQFGAIVLRANTDGSTVRIKDVARIELGAQSYA
                     ISARLNGRPSTGIGVQLSPTGNALATADAVRKRMQELSAFFPSGVKWTIPYDSSRFVK
                     ISITQVVITLLEAVALVFLVMFLFLQNWRYTIIPTLVVPIALLGTFGALLALGFSINV
                     LTMFGMVLVIGIVVDDAIVVVENVQRNIEAGLSPREATYRAMREVSGPIIAIALALVA
                     VFVPMAFISGLSGQFYQQFALTIAISTVISTVNSLTLSPALSALLLKAHGAPKDALTR
                     GMDRTFGWLFRGFNALFGGGAHAYRGGVARVLSRKALMMALYLFLVAATALLFRAVPG
                     GFVPQQDKGYLIGFAQLPDGATLDRTEDVIRRMGDIMIEQPGVENAVAFPGLSINGFT
                     NSSNAGIVFARLGEFEDRRSPALSGGAIAGQLNQKFAAIPDAFIAIFPPPPVQGLGTT
                     GGFKLQLEDRASLGYEALDGATKSFLQKATQTPELAGMFSSYQVNVPQLYADVDRTKA
                     RQLGVSVTDVFDAMQIYLGSLYVNDFNRFGRTYSVRVQADAPYRARAEDVGLLKVRSS
                     SGQMVPLSALMSVSPSSGPERAMRYNGFLAADINGRSAPGFSSGQAQDAVERVAAETL
                     PAGIGFEWTELTYQEILAGNSAVWVFPLSVLLVFLVLAALYESLVLPLSIILIVPMGL
                     LAAMSGVWLTGGDNNIFTQIGLMVLVGLSAKNAILIVEFARELEFAGRTPVQAAIEAS
                     QLRLRPILMTSIAFIMGVLPLAFSTGAGAEMRQAMGVAVFSGMIGVTAFGIFLTPVFY
                     VLLRAATGNRPLKRHGEVPHDVEAFAGGGALQAGPAAPLRKDMS"
BASE COUNT         2826 a         6163 c         6234 g         3131 t
ORIGIN      
        1 cacatggggg aaggggctag gggccagtga ctggggccag cggcgataga ttaagggctg
       61 ggggctttgg ccggagcgcg ggtagttgct ccctctcccg cttgcgggag agggctgggg
      121 tgagggccgc ccgccgtgaa ccgcaccgcc gtaccttcgc gcagcaggcc gatgttgccg
      181 cggaccacca ccgtgccggc cgcgaggccc ttcacggcaa cgatcacttc cttgcccgat
      241 tcgccgcgag cgccgagctc caccggcttg tggacgatct ggttgttttc gactgcctgc
      301 acgtaaggcg ccggcttgtc ggtgcgcacc gcggacagcg gcaccgccag ggcctcagtc
      361 cgcccggtcc ccagcgtgcc ctgggcgaac aggccctggc gcaggccgga cgcgtcggcg
      421 atgaccaggt aggccagcac gctgcggctg ccggcttgcg cgctcggatt gatgcgcgcg
      481 accctggcgc gcaccggccg gttgctgcct tcgatctgaa gcagcgcttc ctgtcccacc
      541 cgcagatcca tcgagtcggc ggggctgagc gtggcttcga gctccaggcg gctcaggtcg
      601 acaatctcga tgacccgcgc ctcgatgccg acccgctcgc cgggctgcgc cagccgctgc
      661 gccaccacgc cggagatcgg agcccgcagc acggtgtcgt ccagcgtctt gcgcgccatc
      721 tccaccgccg ccagcgccgc cttgtgcgtc gcctgtgcgg ccgcgaagtt gttccacgac
      781 gtatccaggg cggttttcga gatgaagccc tggtccacca gggccttgtt gttgtcccat
      841 tggcgctgtg cgctgtcgat ctgcgccttg gccgcatcgg cctgctcctg ggcctgcttg
      901 acccgggcgt tgtactcggt ggcgtccacc cgggcgatca cctggccggc cttgaccgcg
      961 tccccttcgc gcaacgcgag gccctgcagt tcaccggccg cacgtgcctt gaccagggcg
     1021 gagttgaccg ccttgagcga gccggagatc ggcaggccct gcgcgagctc gcgcgtttcg
     1081 gccttcacga cgtcgctgtc ggccagctcg accacggtct ggaccttcgg gatgctggcc
     1141 tgcgccacgg cttcctgctg ggccttgcgc gccgagagcg ccttcatcac gctgaatgcg
     1201 atcgccagca cgaacagggc caggcccagc cattggtatg tgcgcttgct catgggttag
     1261 cccgtccgtc ttttttgccg cccttgtctt ctggtcgcac gcacaggccg cgcaacagcg
     1321 tttcgacctg cgaagcgaca tagcgctcgg ggtccagcgg gaagtcccgc ggcacacagg
     1381 cccccatcga atgcttcatc atcatgagaa agaccatcgg cgccgtgacg gtgaagagcg
     1441 cgtagtccat gtccggcacc tcgaactcgc cgcggtccac cccccgctgc agcatcttgc
     1501 ggatcagaag gtgggccggc tggatcacct cctgctggta gaaggcggcg atgtcgggga
     1561 agtttcgcgc ttcgctgatg atgagcttgg tcaggcccga ggccttggtg gcgccgatcc
     1621 gctcccacca catgcgcatg aagtggcgca gcatgtccgg cgtgctgccc tggaattcct
     1681 cgaactcctt gttccatccg gggaagcggc ccgagatgtt ctcgcgcacg gccgccttga
     1741 acaggtcttc cttgctcggg aaatacagga acagcgtgcc cttggagacg ccggcgcggg
     1801 cggccacttc ctcggcgcgg gtggcggcga agcctttttc gacgaacagg tcgagcgcgg
     1861 catcgagcaa ctcacccggg cgcgcttcct tgcgccgctc gcgcttggcc tgcacctctt
     1921 cctgaggcat accgatgagt ttggaaaggg acattgagtt aatgactgac tggttagtaa
     1981 tataactttg aaggtccccg gccgtcaagc cgccaagttg atgaagttgc gccagatcat
     2041 tgttttcaca cggtaaatat tcggtaaacg gccttggcgg cctcgtgctg cgcgagcagt
     2101 tgcaggcgcc agaaccggga cgtgtcaacc gtggtagtga cttgcgggtt tcctgaacgg
     2161 cgtcctcttc tgagcgagcc cgaatttctc aagtgaagag cgcaagcatc gcgccgaggc
     2221 cgggccggta agattcgcca ttccaaagcg acctacgttc tttccggaga accaccatgt
     2281 ccacttcctc gcaaaccatc gtcctgggcg gcggctgctt ctggtgcacc gaagccgttt
     2341 acgtgaaggt gcgcggaatc accgacgtgg agtcgggcta cagcaacggc catgtgccgc
     2401 agcccacgta cgagcaggtg tgcacgggaa ccaccgggca caacgaagtc gtcaagctca
     2461 cgtacgaccc atcgcagatc agcgtgcgac aggtcctgga gatcttcttc gtgatccacg
     2521 accccaccac gctgaaccgg cagggcgccg acagcggcac gcagtatcgc agcggcatct
     2581 accacaccac gccggagcag aaggacgtcg ccgacgacat gatccggcaa atgagccagg
     2641 acaagctgtt cggcaagccc atcgtgaccg aggtgctgcc gctcgcaaac tactggccgg
     2701 ccgaggagta ccaccaggac ttcttcgaga agaatcccta ccagggctac tgcatggcgg
     2761 tggccgggcc caaggtcgcc aagttccgca agacctttgc cgaactggtg aagcaatagg
     2821 tcccgcacgt cgatggtcga aacccggggt ttgcgctatc aatatccggg cggcccggcg
     2881 ctctcgttcg atgacctcga cgtgccgcaa ggcgccacct tgctgctgca gggcgtctcc
     2941 ggggccggca aatcgacctg gctggctctc gccgccggct tgctcacccc cagcgcgggc
     3001 gaagcggtga tcgcgggcca gcggctgtcc gcgctctcgc gggccgcgcg tgacgcatgg
     3061 cgcgggcgcc acctgggctt cctgccgcag aagctccacc tgagcgaggc gctgaccgtg
     3121 gagcgcaaca tggcgctggc tttctatgcc gcgggtgtgc ccgaaatccg cggcacggtg
     3181 caaaccgcat tgaatctcct gggcgtgggt gagctggccg gccgccggcc gtcccagctg
     3241 tccggcggcc aggcccaacg cgtggcgctg gcgcgcacca tcctgatgga gccccgcatc
     3301 atcctggccg acgaacccac ggcgagcctg gacgacgagg ccgcgtcgac gggcttgaag
     3361 ctgttggaga actgcgcgtt ccagtgcagt gccacgctgg tgatcgctac ccacgaccgg
     3421 cgcgtgcagg aggcattgcc ggccgcgatg gtctaccgga tcggccaccg ggcggcgcag
     3481 ggcagcgtgg ccgccgcatg aaaaccatcg cgctcgcctt ccgctatctc tggtcgcggc
     3541 cgctgcaggc cctgctcaac ctgctgctgc tcacgctggg cctcgcatcg atcaccttcg
     3601 tgctgctggc cagcgagcag atcgaccgcg cattccagcg cgacctggcc ggcatcgacc
     3661 tggtggtggg cgccaagggc agcccgatgc agctgatcct ggccggcgtg ttccacatcg
     3721 acgtcccgcc cggcaacatc tcgcttgccg acgtggagga attgcgccgg cagccccagg
     3781 tggccaagct gattcccttg agcctgggcg acgccttccg cggctttcgc atcgtcggca
     3841 ccaacgccga ctacctgtcg cactaccgca tggcgtttgc ccaagggcag ccctgggcgc
     3901 aaagcatgga agccgtgctg ggcgcgcagg cggcgcaggc caccgccatg aaaaccggcg
     3961 acagtttcat cggcagccat ggtctgggcg gcgggggaca cggacacggc gagcaccctt
     4021 acaagatcac cggcgtgctg gcgccctgcg gctgcgtggt cgaccggctg atcctcacgc
     4081 ccaccgaatc cgtctggcag gtgcacgaga aagccatggc ggtggacgat gaagaccgca
     4141 aggccctgga ggccgaacgc gagatcacga tcgcgctgat cacctacaac agcccgctcg
     4201 ctgccgtcac ttttccgcgt tatgtcaaca gccgcaccga catgcaggcc gccgcgccgg
     4261 ccgtggaagt gtcgcggctg ctgcgcatgc tgggcgtggg ctccgaggtg ctgcgcggcg
     4321 tcggcgccgt gctgctgctc accgcggcct tgagcgtctt cgtcgccctg tggaacgcgg
     4381 tgcgcgagcg ccgggccgac ctggccatgc tcaggatgct gggcgccacg ccggccaagg
     4441 tcgcggccct gctggtttgc gaagccttgt ggctggcggt gctggcctcg gtgctggggc
     4501 tcgccgccgg ccatggcttg acggccctgg taggctggtt gctggaaggg cagcgttcgc
     4561 tgccgatgag cggccggctg tgggtgccgg ccgaggcctg gattcccgcc gcggcggtgg
     4621 gcgtggccgc gctggcggcg ctgattccca cggtgagcgc ctatcgtgtc gatgtggccc
     4681 acctgctcaa cacgaggtga acgaatgatg aaacagctta tgtttttggc cgcctggctg
     4741 ccgctgctgg cgctggcggc cgagagtccg gaagagatca agaaggatgc tcagcggcat
     4801 cgggccatgg ctgtcgcgca cgaggcggca gccaaatgcc tggaatcggg caagaacccc
     4861 gaccaatgcc agaaagacct gcaggctgcc tgtaaaggcc tggctatagg aaaatactgt
     4921 ggaatgaagc acgcgcattg atcgggccgt gtcggctgcg ccgctgcacg gtccattgcc
     4981 ggtccgatgg tgcttcccgg gagtacgcca tgaagagaat cgttttagtg ctggcagcgt
     5041 tgttcgccgc gtccgcgggc gcgcagctaa gcccaccggt gcccttgggc acggggccgg
     5101 gcgtgcacag ccccaacagc ccgtttgcgc cgctgcccgc gcgagacgac gtggtgccgt
     5161 ggtcgctgct gacggccgtg aagaccaaga ccgaaaagaa caaggtgctg cccgtgttcg
     5221 acaagccgca gcttgcgctg aacgcgaaga cccagcgcgt ccagggattc atgatgccgc
     5281 tggagccggg cgaaaagcag acccacttcc tgctcagttc gataccgctg acctgcgctt
     5341 tctgcgtgcc gggcggcccc gaaagcatgg tcgaggtcaa gaccaggacg cctgtcaagt
     5401 acacgctcga ccccgtcacg gtcgagggca tgttcgccgt gctgaacgac gacccgtatg
     5461 ggctgtacta ccgcatcacc gacgcggcga gcatcaggta aatgacgagt ttctgaattc
     5521 gctcgaaaag tttcccggct gaattagcgc ttcgcggcgt ggtcgcgtat ccacgccgcg
     5581 aactcggctt ccgtgatgtc gccgctggcc acggcgagca tggtcaccac gcagtcgacg
     5641 tcgtccgccg ccagcgcgtg gcggttgagc tcgagaaaca gttccgtcgc gacaaaaccc
     5701 gttctcttgt tgccatcgat gaaggcgtga tttcgcgaaa taccgtagcc ataagccgca
     5761 gcaagatcgg cgatatcagg tttgccatag gccgccaggt tttccggtcg ggctaaggcg
     5821 gactccagca agccttggtc ccgcactccg gccgcgccgc cgtgttcggc caactgatgt
     5881 tcatgtacgg cgaggatgat ctctcggtgc agccagaccc agcgcttcat ttagccagtt
     5941 cacgcagaac tgcccgcctc tttttcatga cgtcccgggc ggccttcatc tgaacctcaa
     6001 actccgggtt atgcaccgtc agccgcagcc cgtcaggtga atccgtcaag tacagttcat
     6061 ctcctttttc gaggttcagg cgcgccagca cttccttggg aaaaattgca ccgacggagt
     6121 ttccgacttg agtcagtttg agcgtataca tggcccagcc ctttgtaata acatccgtta
     6181 tactaacgtt gatccccggg aagtgcaaca cgagatgaat tcgagcgtcc cctccgctct
     6241 cgtctcgcac tgctgcggcc gcatgaccgt gtcgcttgtc ctgacagcga gctctgcgct
     6301 gtgactacgg cgccagccgt tcgcgcaccc agttgccgcc ttcgagccgg tagcgcagcc
     6361 tgtcgtgcag ccggctcttg cggccctgcc agaactccca ccggtccggc accagccggt
     6421 agccgcccca atgcgatggg cgcggcgggt tgagcaggta ctgcgcgccg aatttcgccg
     6481 cgttggcaac cagcagcgtg cggctggcga tcacctggct ctgcgggctg gcccaggcgc
     6541 cgatgcgcga gtccagcggg cgcgtggcgt aataggtgtc gctttcttcg gcgctcgttt
     6601 tctcgacccg gccttcgatc cgcaccacgc gttccagctc cacccaatgg aactgcaggg
     6661 ccgcgtaggg atttcctccc agttcctggc ccttgcggct ctcgtaattg gtgtaccaga
     6721 cgatgccgcg ctcgtcgaag cccttgatca ggacgatgcg ggtgctggga cgcaggtcgc
     6781 cggccaccgt cgccagcgtc atggcgttgg gctcgggaat ttccgcgctg atcgcttcgg
     6841 ccagccattg gtcgaactgc ttcagcggat ccgcatacga agcctcctca ctgagttcgg
     6901 cgcgctcata gctctttctc agttcggcca gccgggaaga agtggtgctc attaagcgat
     6961 tttcgcactg tggacggcgg gaaccgttgt acattgacaa ggggtgtccg ttgacggaaa
     7021 ccagaggaaa ttgcaatgga gagcctggat ctgcgtgtgc ttggcgacgc cctggcctgg
     7081 cggcgagccg gccacgcggt gacgctggtg accgtggtgg aaacctgggg cagcgcgccc
     7141 cggccgcccg gcgccctgct ggccgttcgc gacgacggtg tcgtgagcgg ctcggtttcc
     7201 ggcggctgcg tggaggatga cctcatcgcg cgcgtcaagg ccggcgagcg caacgccgcc
     7261 ccctcgatga tcgcttacgg cgtgagcaag gacgaggccg cccgattcgg cctgccgtgt
     7321 ggcggcacgc tgcggctggt gcaggagccc ttgcgcgaaa ccggctgggt cgaagagctg
     7381 ctcgcccgca ccgccgcgca cgaactggtc gccaggacgc tgcagctggc cagcggccag
     7441 gtcacgctcg cggccgcgtc ccgcgatcaa agcatgcggt tcgacggggt cgagctcacc
     7501 accttgttcg gcccgaagtg gcggctgctg ctggtgggag ccgggcagct gtcgcaagcg
     7561 gtggcgcaaa tggcgcagat gctcgacttc gaagtgatgg tgtgcgatcc gcgcgaggaa
     7621 tattcgaaca cgttcgccgt cacgggcgcg aggcacgtgc ccggcatgcc ggacgacgtg
     7681 gtgcgggaca tgcggcccga tgggcatacg gcgatcgtcg cgctcacgca tgaccccaag
     7741 ctcgacgaca tggcgctgct cgaggccttg cgctccgatg ctttttacgt cggcgcgctg
     7801 ggttcatcgc gcaaccaggc ggcgcgcaag gcacggctgg cggagcactt cggcctcacg
     7861 ggggaagagc tggcccgcct gcatggaccg gtgggattgc gcataggcgc caagaccccg
     7921 gccgagatcg cggtgtccat ccttgggcag atcgtcgagt gcaagaatcc cgccgcgcaa
     7981 gcgctcaggc cggctgcggg ctgcgccgtg acatgaacca gccggtcgtg ctcgtcctgg
     8041 cctccggccg cggcgagcgc tttgtcgctt ccggcggcag cggctccaag ctgcgcgcgc
     8101 tgctgggcgg caagcccgtg ctggaacgca cgcttgacgc cgtgcgggcc agcggcctgc
     8161 cgtggcacct cgaggacgcc gggcatgaag gcatgggcga ctccatcgca gccgcggttc
     8221 gcgccaccca gggcgctccg gggtggctgg tactgcccgg cgatctgccg ctggtgcgcc
     8281 cgcagacatt gcaggcggtg gccgcggcgc tggccgatca cgtggtggtg ctggcccact
     8341 atcggggcca gcgcgggcat cccgtcggtt ttgccgcctc ctgccgcgat gccctgttat
     8401 tgctgcaagg atcgcagggc gcggcgctgg tggtgcgcgc gcaggctacc gtgaaaccgg
     8461 tcgctcaact cgaactcgac gatcccggca tcgtcaccga tatcgacacc ctgcaggacc
     8521 ttgtgaacgc cgaacggctg ctggcggggt aaagcggtct ccccgacggc tttggaggac
     8581 ggcacaccgt tgtggcaaag gcggagccgg gcgggccgcg gcgcatccac gaagcggtcg
     8641 gcgcttgcgc accgaactca tggcatgtca ttgcgggctt gacccgcaat ccaggaagtc
     8701 ctggatgccg gatcaagtcc ggcatgacaa ctcatagcat gttcgaggcg cttgacggga
     8761 tgccggatca agtccggcca cagtgtgagc ccccgccggg taccgcctgc cgcggccccc
     8821 caatccgatg cagcccagcg cgtcagagct tctctttcgc acactcctca gcccctcgcg
     8881 atcaccgcgc aggccaggcg cgggcccgcg ttgccggtgg gctgggtcgt gaaatcgtcg
     8941 ggatcgcggt gcacgatgag gcccttgccg accacgtccg tcgggccgct tccgaccttg
     9001 accgttctcg attcgaagct gaaggccgcc acgccgttcg cgtccgctct caggccgggc
     9061 aggtcgcccg catggtgcac gccctgtccg tgccgccgtg gggcttgcca tcgggattga
     9121 agtggccacc ggcgctgttg cccgtccggg ctggagcagt cgcctttctc gtgcacatga
     9181 aagccgtgct cggcccccgg cttgagcccg cgcacttcgc cactgacccg caccacgtcg
     9241 cccgattgca cgaaccgcac cgtgccggcg gtggtattgc ccgctgtcgg cctcaggctc
     9301 gcgacggcct gcggaccttg tagggctgcg cagcccgcca gactgcgagc gcggcagcgg
     9361 cggcgacgac gaagcggaat gtgtctatgg ccatggcggg tctcctgttg ttgcttgcgt
     9421 tgcggactgt agaggctgtc atggcctctg acaatggggg aaggcctccc cggccgatct
     9481 catcagcgct gcatcagctt gagcaaccgc tccagtccgc ggtcccggat gttgtgccgg
     9541 cgcagctctt ccaaggtgcg atgcgcgtag tccagggtgc tgccgtagat gccggaggcc
     9601 tcggagaaga tgagccggta ttgcgcctcg gtgagcgcgc cggtgtggct ggggctcttg
     9661 cgcgacagcg tgaaggccag ggccgtgacg gggccgtggc tggtgtggct ggtgagccat
     9721 cgcggatcgt agacgcccat gatcatctcg cgcgaccaca ggtgggtgag tacctgctgg
     9781 ccggcttggc gcggaatgcg gaacaccatg ccctggcagc atccgcccga cagcatgccg
     9841 aacacgaggc ccggccgttc cggcgtgccg cgattgatcc ggctccacat cttgagggcc
     9901 cgatgccagc catgcaccct ggccggccgg cgctccgcga attcgaaatc gggcctccag
     9961 atcagcgagc cgtagccaaa gatccacagg tcttcgtggc caccccattc ctgcagtacc
    10021 cgctcgagca tgggcgcggg gtcgcgcagc ggcttgatcc catgcggccg gaatggctgc
    10081 cccgtcgagg tggacgtggc ggggaagcct tcggactggt cgtcggccgg actggagcgg
    10141 ttctgcgcag ggctcatggc gcgaacttta acgtcgcgcg cgtcgccgcg ccttggtaac
    10201 ctgttcgtaa ctgcggcaca catcgcagcc cgggttccga ggtaggatca ccggctgatg
    10261 taacttagtt accaatccac atgaaaccgc atcctgttct cgccgccgtg accgagcgca
    10321 tccgtgagcg cagcgcgccg cggcgcgccg cctacctgcg ccagatcgac gaagccgccg
    10381 gacgcgaccg tggcgccgac cgcatgggct gcgccaacgt ggctcatgcg tttgccggcc
    10441 tgccgccgaa cgacaagttc cgggtcgtgg ccgagaaggc tcccaacatc ggcatcgtca
    10501 acgcgtacaa cgacatgctt tccgcgcatg caccgctggg acgcttcccc gacctcatca
    10561 aggaggaagc gcgcaagcac ggcgccacgg cccaggtggc cgggggcgta cccgccatgt
    10621 gcgatggcgt cacccagggc acggccggca tggagctgag cttgttctcg cgggacgtga
    10681 tcgcgatggc gacagcggtg tcgctcagcc acgacgtgtt cgacgcggct ctgatgctcg
    10741 gcatttgcga caagatcgtg ccgggcctgc tgatcggggc gctgcacttc ggccacctgc
    10801 cgatggtgtt cgtgccgggc gggcccatgc ccagcggctt gtccaacagc gagaaggcca
    10861 aggtgcgcga gcaggccgcg caggggctgg tcgggcgcga agcgctgctc gacgccgaat
    10921 cgcgcgcgta ccacgccccg ggcacctgca cgttctacgg cacggccaac agcaatcaga
    10981 tgctgatgga agccatgggc ctgcatgtgc cgggcacggc cttcgtcaac ccgggcaacg
    11041 aactgcgcga tgaactcacc cgggaagcgg ttcgcacggt actggatatt ctcaagggca
    11101 ggcgctttgc cccgatcgga cggctggtcg acgagcgcgc cgtagcgaac gcgatggccg
    11161 ccttgctggc caccggcggc tcgaccaatc acctgatcca ttggatcgcc gtggcgcgtt
    11221 cggccggcat cacgatcgat tgggacgatt tcgcgcagct gtcggccgtg gtgccgctgc
    11281 tggcccgcgt ctatccgaac ggcagcgcgg acgtgaacca gttccaggcg gcgggaggtc
    11341 ccggcttcgt catccgcgag ctgctggacg ccgggctgat gcatgaagac gtgctgacgg
    11401 tgcgttccgg cggcattcgc gaatacacgg gcgaacccag gctggccgac gcgggcctcg
    11461 cctggagtcc catcgggccc tcgcgcgaca cggacatcgt gcgccctgcc gccgatccct
    11521 tcagcgccac cggcggcctg aagctgcttg ccggcaacct cgggcgcagc gtgatcaagg
    11581 tctcggccgt gccggccgac cgccacctga tcgaagcgcc ggcccgcatt ttcgacgcgc
    11641 aggaggaact gcagcgtgcc ttcaatgccg gggagctcga tcgcgatgtc gtgtgcgtgg
    11701 tgcgctggca gggtccccaa gccaacggca tgcccgagct gcacaagctc acaccgccgc
    11761 ttgcggtgtt gcagggccgg ggctttcgcg tcgcgctggt caccgacggg cggatgagcg
    11821 gtgcctcggg cagggttccc gcggccatcc acgtgtcgcc cgaggccgct gccggcggcc
    11881 cgctggccag gctgcgcgac ggcgatgcgg tgcggctgga cgcggatgcg ggggtcttgc
    11941 aggcgctggt gcccgcgcat gaatgggcgg cgcgcgagaa tgcggccatg cctgaagcgc
    12001 tcagttccgc caatggcctg ggcatcggcc gcgaattgtt cgcggcgttc cggcgcaacg
    12061 cgctgccggc cgaggaggga gcctgtacat ggctgtaggc aatgaacagt tcgccgccgg
    12121 tcggctgact tcgctgcagg tgatgacgga tgcgccggtg atcccggtga tcgttctcac
    12181 cgacgccgcg cacgccgtgc cgctggcgcg cgcactggtc gccggcggca tccggatgct
    12241 ggaagtgacg ctgcgcacgc ccgcggccct ggcttgcatc gaggcgatcg cgcgcgacgt
    12301 gcccgaggcg atgaccggcg ccggcacggt gcgcagcgcc gccgatgcgc aggccgcggc
    12361 tatggccggc gcgcgcttcg cggtcagccc gggctatacg catgcgacag gcagggcctg
    12421 ccacgacctg gagttgccgc tgctgcccgg ggtggcgacc ggcagcgaga tcatggcggc
    12481 gcaggcggac ggctacacgg cactgaaatt ctttcccgcg atgccagccg gcggcgtggc
    12541 catgctcaag gcctggcagg gcccgttcgg cgacgtgacg ttctgcccca ccggcggcat
    12601 cacggccgcc aacgcgccgg acttcctggc gctcgccaac gtggtgtgcg taggcggctc
    12661 ctggctcaca ccggccgatg cggtggcgag cggcgactgg gcgcgcatca cgcagctcgc
    12721 acgcgaggcc gtggcattgc agccatcgcg ctgagcggat gcccggcgtt tacaccgact
    12781 cggtggaccg ctggatcagc tcgatcttgt agccgtccgg atcggtgacg aaagcgatca
    12841 ccgtcgtgcc gcccttgaca ggccccggct cgcgggtgat gttgccgccg ccggcgcgga
    12901 tcttctcgca cgccacgtac acgtcgggca cgcccagtgc gatgtgccca taacccgtcc
    12961 ccaggtcgta gcggtccacg ccgtagttgt acgtgagctc gatctcggca tgctcgggat
    13021 tgctgccgta gcccacgaag gcgagcgagt agttctgctc gggccgctcg gtggtgcgca
    13081 gcagcgtcat gccgagcaca tgggtgtaga agtcgatcga gcgctgcagg tcgcccacgc
    13141 gcagcatggt gtggaggagt ctcatgcggt gattatggca agtccgcgtg cgccgcggcg
    13201 aagcgcgagg tatgctggga cggttcgccg gcaagccacg aacacagcca tgatcctcct
    13261 tcccagcgtg cgcctggcca ccccccacga tgcccgcggc atcgcggaga tgtcgcgcga
    13321 gtacatcgag catggcctgg gctggacctg gacgcgtgag cgcgtgctca aggccatcga
    13381 tgagcgatcg accaacgtcg cggtcgtccg cgaggaccat cggctcctgg gcttcggcat
    13441 catgcagtac ggcgatcaaa gggcgcacct gtcgctgctg ggcgtgcagg cgagccagcg
    13501 ccagcgcggg ctggcaacgc tgctgctgtc ctggctcgag aaatccgcag cggttgcggg
    13561 catcgaacgc atccagctcg aagcgcggtc ggacaatgcg atcgccctgg ccttctaccg
    13621 cgggcagggc taccggcaga cgggcgtgat caccgggtac taccgcggcg tgctggatgc
    13681 ggttcggctc gaaaagaagc tggggcccac ggcggaaagc tgatcgcgtc gcccggattg
    13741 cctgccgcac agtggacgcg aacaggcttt ggagatattc cttatactgg ttcagccgct
    13801 tttttcctgg tggcgcagcg ccgcgatcct ggtccggcac ctccattgcg aatcttgaag
    13861 ttcatcccca tggccctttc gtgtgcccac cggcttcatc ggctgctcgc cgttgccggg
    13921 ttcacctgct tgttgtccgc ctgcgggcag ggcggaagcg cacagtctgc ggcacccgcg
    13981 ccgccggccg aggtcggcgt ggtcaccgtc gctcccggtg acgtggggtt ggtgaccgag
    14041 ttgcccggcc gcctggaggc ctcgcgcgtg gcgcaggtcc gcgcccgcgc cgccggcatc
    14101 gtccagcggc ggctgttccg cgagggcagc gacgtgcgcg cgggccaggc gctgttcacc
    14161 atcgaccccg cgccctacgc ggcggcgcat gccagtgccc aggccgcgct ggcgcgcgcc
    14221 caggccaatc tgggccaggc cgcggcactg gccgagcgct acaagccgct ggtcgaagcc
    14281 aatgccatca gcaagcagga atacgcgaac gcggtggcgg ctcgcaagca gggcgaggcc
    14341 gaggtggccg cggcgcgggc agcggtgcag acggcgcaga tcaatctggg acatgcctcg
    14401 gtcacggcgc cgatttccgg ccgcatcggc cgggcgctgg tgacggaagg cgcgctggtg
    14461 gggcagggcg aggcgacgcc gctcgccgtg atccagcaga tcaatcccat gtacgtcaac
    14521 ttcacgcagc cggcaaccga agtgctcaac ctgcggcgcg cgttcgagca ggggcgcctc
    14581 aagcgtgcga tcggcgcgga aggcgcgctc gtgcgcgtgg tgctcgaaga cggtagcgag
    14641 catcccaatc ccggccgctt gctgttctcc gacctgtcgg tcgacccgac cagcggccag
    14701 atcaccttgc gcgccgaggt gcccaacccc gggggcgccc tgctgcccgg cctgtacgtg
    14761 cgggtgcggc tggagcaggc caaggccggc aacgccgtgc tgttgccgca gcaggccgtg
    14821 acgcgctccg cccagggcga caccgtgatg gtggtggcgt ccgatggcca ggtcagcccg
    14881 cggcccgtga aggtgggcgg ctcgcaagcc gggcagtggg tcatcctcga gggtctcaag
    14941 cccggcgagc aggtgatcgt ggacgggttc cagaagctgc ggcccggcgc cgccgtcaag
    15001 ccggtgccgt ggcgcgcggg gcccgcagct gcggcaacgc cggcgtccgc gcccgctgcc
    15061 gcggccaagt cgtaaggagc tcgccgcatg gcaaagttct ttatcgaccg gccgattttc
    15121 gcgtgggtga tcgcgctgtt catcatcgtg ctgggcggcg ttgccatcac ccagctgccg
    15181 attgcgcagt acccgccggt ggcgccgccg tcgatcgtca tctccaccaa ttatcccggc
    15241 gcgtcggccc gaacgctgga ggaaagcgtg atcagcgtga tcgagcagga gatgaacggc
    15301 tcgcccggcc tgatctacat ggagtcggtg agccaggcca atggcagcgg ccagatcacc
    15361 ctgagtgtcc agcccggaac gcctcccgac ctggcgcagg tcgacgtgca gaatcgcctg
    15421 tcacgcgcca gcccgcgcct gccgcaggcg gtgacgcagc agggcgtgcg ggtcgacaag
    15481 gcgagatcga acttcctgct cttcaccatc ctgtcgtcgg acgatccggc cttcacgccg
    15541 gtggcgctgg gcgattacgc ctcgcgctcg gtattgcccg aaatccagcg cattcccggt
    15601 gtcggccagg cccggctgtt cggcaccgag caggccatgc ggatctggat cgacccggcc
    15661 aagctgctgg gcttcaacct ctcgtcggcc gaagtcaacg ccgcgatccg cgcgcagaac
    15721 gcacaggttt cttcgggctc catcggcgac ctgcccaaca tcgcgggcca gggcatcgcc
    15781 gccacggtcg tcgtcaacgg ccagctggcc tcggtcgagc agttcggcgc catcgtgctg
    15841 cgcgccaaca ccgacggatc gacggtgcgc atcaaggacg tggcacgtat cgagctgggc
    15901 gcgcaaagct acgccatctc cgcgcgcctg aacggccggc cgtccaccgg catcggcgtg
    15961 cagctctcgc ccaccggcaa cgcactggcc acggccgacg cggtgcgcaa gcgcatgcag
    16021 gagctgtccg cgttttttcc cagcggggtg aaatggacca tcccttacga cagctcgcgc
    16081 ttcgtcaaga tctcgatcac gcaggtggtg atcacactgc tcgaagccgt ggcactggtg
    16141 ttcctggtga tgttcctgtt cctgcagaac tggcgctata ccatcatccc gaccctggtg
    16201 gtgccgatcg cgctgctggg gactttcggc gcgctgctgg cccttggctt ctcgatcaac
    16261 gtgctgacca tgttcggcat ggtgctggtg atcggcatcg tggtggacga cgccatcgtc
    16321 gtggtggaga acgtgcagcg caacatcgag gccgggctct cgccgcgcga ggccacttac
    16381 cgggcgatgc gcgaggtgtc cggaccgatc atcgccatcg cgctcgcgct ggtggcggtg
    16441 ttcgtgccca tggcgttcat cagcggcctg tcgggccagt tctaccagca gttcgcgctc
    16501 accatcgcca tctccacggt gatttcgacg gtcaattcgc tcacgctctc gcccgcgctc
    16561 tcggcgctgc tgctcaaggc gcacggtgcg ccgaaggacg cgctcacgcg cggcatggac
    16621 cggaccttcg gctggctgtt ccgcggcttc aacgcgctct tcggcggcgg cgcgcacgcc
    16681 taccgcgggg gcgtggcgcg ggtgctctcg cgcaaggcgt tgatgatggc gctctacctg
    16741 ttcctggtgg ctgccacggc gctgctgttc cgggccgtgc cgggcggctt cgtgccgcag
    16801 caggacaagg gatacctgat cggcttcgca cagcttccgg acggggctac gctcgaccgc
    16861 accgaggacg tgatccgccg catgggcgac atcatgatcg agcagcccgg ggtcgagaac
    16921 gccgtcgcct tcccggggct gtcgatcaac ggcttcacca acagctccaa cgcgggaatc
    16981 gtgttcgcgc gcctcgggga gttcgaggat cgcaggagcc cggccttgag cggcggcgcc
    17041 atcgccgggc agctcaacca gaaattcgcc gcgattcccg acgccttcat cgccatcttc
    17101 ccgccgccgc cggtgcaagg gctggggacc accggcggct tcaagctgca gctcgaggac
    17161 cgggcttcgc tcggctacga agcgctcgac ggcgcgacga agagcttctt acagaaggcc
    17221 acccagacgc cagagctcgc cgggatgttc tcgagctacc aggtcaacgt gccgcagctc
    17281 tacgccgacg tcgaccgcac caaggcacgc cagctgggcg tctcggtgac cgacgtgttc
    17341 gacgccatgc agatctacct cggcagcctg tacgtgaacg acttcaatcg attcgggcgg
    17401 acctactcgg tgcgcgtgca agcggacgcg ccgtaccgcg cgcgcgccga ggacgtgggg
    17461 ctgctcaaag tgcgctccag ctcgggccag atggtgccgc tctcggcgct gatgagcgtg
    17521 agcccgagct ccggacccga gcgcgccatg cgctacaacg gcttcctcgc ggccgacatc
    17581 aacggccgca gcgcgcccgg tttctcctcg ggccaggcgc aggacgcggt cgagcgcgtc
    17641 gccgccgaga cgctgccggc gggcatcggc ttcgaatgga ccgagcttac ctaccaggag
    17701 atcctcgccg gcaattccgc ggtctgggtg ttcccgctgt cggtgctgct cgtgttcctg
    17761 gtgctcgcgg cgctctacga gagcctggtg ctgccgctct cgatcatcct tatcgtgccg
    17821 atggggctgc tcgcggcgat gagcggtgtg tggctcacgg gcggcgacaa caacatcttc
    17881 acgcagatcg ggctgatggt gctggtgggg ctgtcggcga agaacgccat cctgatcgtg
    17941 gagttcgcgc gcgagctcga gtttgccggc cgcacgccgg tgcaggcggc gatcgaggcg
    18001 agccagctgc ggctgcgccc catcctcatg acctcgatcg ccttcatcat gggcgtgctg
    18061 ccgctcgcct tctcgaccgg cgccggtgcc gagatgcgcc aggcgatggg cgtggcggtg
    18121 ttctcgggaa tgatcggcgt caccgccttc ggcatcttcc tcaccccggt gttctacgtg
    18181 ctgctgcgcg ccgcgactgg caaccggccg cttaagcgcc acggcgaagt gccgcacgac
    18241 gtcgaggcgt tcgcgggcgg aggcgccttg caggcgggcc cggccgcgcc gctccggaaa
    18301 gacatgtcat gagattcgtt gttttgggct tgctgctcgc cggctgcgct tcca
//
LOCUS       DASWIY010000309         7644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3518148, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000309 DASWIY010000000
VERSION     DASWIY010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3518148"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VGA59_11315"
     CDS             <1..1333
                     /locus_tag="VGA59_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9720311.1"
                     /translation="SGKWTGTMNLTEPQAGSDLAMVRTRAEPQPDGTYKIFGTKIFIT
                     YGEHDMAQNIVHLVLARVTGAPEGVKGISLFVVPKFMVGKDGSPGARNDVHCVSIEHK
                     LGIKASPTAVLQFGDHGGAAGYLVGQENRGLEYMFIMMNAARYAVGVQGIAIAERSYQ
                     QAVQYAKERIQSRPVDGSLNASAPIIHHPDVKRMLMTMRACTEGCRAMASVAAAAYDA
                     AHHHPDPQARKQNQAFYEFLVPLVKGYSTEMSIEVTSLGLQVHGGMGYIEETGAAQYY
                     RDARILTIYEGTTAIQANDLVGRKTARDGGQVAKAIAAQIAKTEAELARRDSVAARAV
                     LKRLAAARQAFIEVVDFVAGNTKASPNAVFAGSVPYLMLAGNVMAGWQLARSLLVAED
                     MAARGEDAEFMRAKIATARFYADHMLSKVPGIRDAIVEGAESVTALPAESF"
     gene            1408..2394
                     /locus_tag="VGA59_11320"
     CDS             1408..2394
                     /locus_tag="VGA59_11320"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX9720312.1"
                     /translation="MSKLTKALQNLALPVIGSPLFIISNPKLVIEQCKAGIIGSMPAL
                     NARPASQLDEWLHEITETLAAYDRAHPHAPAAPFAINQIVHKSNDRLEHDMEMVVKYK
                     VPVIITSLGARTDVNAAIHSYGGIVLHDIINNAFAHKAVEKGADGIIAVAAGAGGHAG
                     VKSPFALIQEIRQWFDGPLVLSGSISTGGAVLAAQAMGADFAYIGSAFIATHEARASD
                     AYKQAIVECDSDDIVYSSLFTGVHGNYLKPSIRAAGLDPDNLPDGDVKTMNFAGGEGS
                     KSKAWKDIWGCGQGIGAVTEVTSAAALVARLKREYRAAQQRLLPQPVVLEAA"
     gene            complement(2429..3442)
                     /locus_tag="VGA59_11325"
     CDS             complement(2429..3442)
                     /locus_tag="VGA59_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX9720313.1"
                     /translation="MNLDWAHLLPLIFMAVMGLALLAYVVLDGYDLGVGILLPFATEG
                     EKDVMVSSIGPFWDANETWLVLGIGVLLIAFPLAHGIVLTALYLPVAVMLIGLVLRGV
                     SFDFRVKARAAQKTTWNRLFTLGSFMAAAAQGWMLGSYLTGFDRSLWPMLFSLGIALT
                     LPTAYAMLGAGWLIMKTGDALQLKAVGWARTVLWPMGAALVGISLATPMVSQTVFDRW
                     FSMPQFIGLLPIPVACAVAFYAVFHVTGRPRVVAAGYGWVVFAGTVLIFVLSFLGLAY
                     SLYPYIVVDRLTIWQAASATKSLIFIFVGVAITLPAIIVYTVFMYRVFWGRASSLTYG
                     PGQ"
     gene            complement(3457..4851)
                     /locus_tag="VGA59_11330"
     CDS             complement(3457..4851)
                     /locus_tag="VGA59_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX9720314.1"
                     /translation="MDTLLLSRIQFAANIGFHILFPTINIALCWFLVYFRLRHSAAGD
                     TAEAQGWEEAYYLWTKIFALTFALGVVTGITMSFQFGTNWPGYMERAGNIAGPLLGYE
                     VLTAFFLEATFLGIMLFGRGRVSDRVHLFATLMVAAGTTLSAFWILSLNSWMQTPAGY
                     EIIDGRFHATSWLAVIFNPSFPYRLVHKLLASALTASFLIAGLSAWQLLRGTANSGTR
                     RALNAGVVAAAIAIPLQIFAGDLHGLNTLEHQPAKIAAIEGIWHTERSAPLTLFGWPN
                     EKERRTDYALQVPKLASLILAHDWEAELKGLDEFPGAHPPVAPVFWSFRVMVGIGVLM
                     LLVSWPCAWTLWRRRAGAPARDDAVPFSRWQLLLLAAMTFSGWVSTLAGWFVTEIGRQ
                     PFMVYGLLRTADLVAPHPVAMVASTLVAYLVVYAFLLASYVLVLMYMASHPAQPTADT
                     LQSAKAALPIGGPA"
     gene            complement(5023..6231)
                     /locus_tag="VGA59_11335"
     CDS             complement(5023..6231)
                     /locus_tag="VGA59_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9720315.1"
                     /translation="MDTTQPTDRKPRILVVDDTPDNIFLMSALLEDEYEVATASGGQE
                     ALRIAQSDAPPALILLDIMMPEMDGYEVMRRLRNDTATAGIPVIFLTALTSIEEEQFG
                     LDLGAVDYITKPISPPVVLARVHAHLERSSNARRLQSLSEKLGRYLAPQVYKSLFDGS
                     RDAEIRTQRKKLTVFFSDIKDFTASTAKWQPEDITFLLNSYFSEMSKIALEYGGTLDK
                     FIGDAMVIFFGDPESRGIKEDALQCVKMAIAMQRRMAELQMLWREMGSDKIFQVRMGI
                     NTGYCDVGNFGSDLRMDYTIIGAEVNLAARLEQAADPGGILISRETYGLVRDEIVAEE
                     QEPLTVKGFADPIESYAVQEEAEVRSNARKVFQWERLGMRVLIDLDRLPSAERAAAAV
                     ELREMADRLH"
     gene            6449..7537
                     /locus_tag="VGA59_11340"
     CDS             6449..7537
                     /locus_tag="VGA59_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX9720316.1"
                     /translation="MDEVRIPLEGLKGRGAATRLAHRFERDTRGAFDDGWATLEAGLA
                     QAHEVPHTEVIWEEARSIMTSNESPDVYFDRSINPYRGCEHGCIYCYARPTHSYLNLS
                     PGLDFETKIIAKRNIAALLRAELSARNYEPRLIAIGTATDCYQPVERELRLTRSVIEL
                     MHETRHAFSLVTKSSGVERDLDLIAPMAAQGLAAVYLTITTLDADLTRKLEPRAAAPH
                     RRLRTLRTLAEHGVPCGVSLAPQIPFLTQDMEQVLEAAWDAGARSAFYHVLRLPWEVS
                     ALFRQWLELHYPQRAGRIMARIHDMRGGKDYDSDFATRMKGTGPWAELIGQRFLKASR
                     RLGFNSGRVDLDTTAFRKPGAAGQGHLF"
BASE COUNT         1422 a         2512 c         2498 g         1212 t
ORIGIN      
        1 cagcggcaag tggaccggca ccatgaacct gaccgagccg caggccggct cggaccttgc
       61 gatggtgcgc acgcgcgcgg agccgcaacc cgacggcacc tacaagatct tcggcaccaa
      121 gatcttcatc acctacggcg agcacgacat ggcgcagaac atcgtgcacc tggtgctcgc
      181 gcgtgtcacg ggcgcgcccg agggcgtcaa gggcatcagc ctgttcgtgg tgcccaagtt
      241 catggtgggc aaggacggct cgcccggcgc gcgcaacgac gtgcattgcg tcagcatcga
      301 gcacaagctg gggatcaagg cctcgccgac cgcggtgctg cagttcggcg accatggcgg
      361 agccgcgggc tacctggtgg gccaggaaaa ccgcggcctc gagtacatgt tcatcatgat
      421 gaacgcggcg cgctacgccg tcggcgtgca gggcatcgcg attgccgagc gcagttacca
      481 gcaggccgtg cagtacgcca aggagcgtat ccagtcgcgg cccgtggacg gctccctcaa
      541 cgccagcgcg cccatcatcc atcatcccga cgtcaagcgc atgctgatga cgatgcgtgc
      601 ctgcaccgaa ggctgccgcg cgatggccag tgtcgccgcg gccgcttacg acgccgcgca
      661 ccaccacccc gatccgcagg cgcgcaagca gaaccaggct ttctacgaat tcctggttcc
      721 gctggtgaag ggctacagca cggaaatgag tatcgaagtg acctcgctcg gcctgcaggt
      781 gcacggcggc atgggctaca tagaggaaac cggcgcggcc cagtactacc gcgacgccag
      841 gatcctgacc atctacgaag gcacgaccgc gatccaggcc aacgacctgg tgggccgcaa
      901 gaccgcgcgc gacggtggcc aggtcgccaa ggctatcgcc gcgcagatcg caaagaccga
      961 agccgagctg gcacggcgcg acagcgtcgc ggcgcgcgcg gtgctcaagc ggctggcggc
     1021 ggcgcgccag gcgtttatcg aagtcgtgga tttcgtggcc ggcaacacca aggcttcgcc
     1081 caatgccgtg tttgccggct cggtgcctta ccttatgctg gcgggcaacg tgatggcggg
     1141 ctggcagctg gcccgctcgc tgctggtggc ggaggatatg gcggcaaggg gcgaagacgc
     1201 cgagttcatg cgagccaaga tcgccacggc acgcttctat gccgaccata tgctcagcaa
     1261 ggtgcccggc attcgcgacg ccatcgtcga gggcgccgag agcgtgacgg cgttgccggc
     1321 ggaatcgttc tagggttcgt gtcgcggctt tgacacgcag cgccgctcga atttgaggaa
     1381 aatcgagttt tattgagcgg gaaatccatg tccaagctaa ccaaggccct gcagaacctc
     1441 gcgctgcccg tgatcggctc gccgctgttc atcatcagca atcccaagct ggtgatcgag
     1501 cagtgcaaag ccggcatcat cggctcgatg ccggcgctga acgcgcggcc ggcctcgcag
     1561 ctggacgagt ggctgcacga gatcacggag acgctggccg cctacgaccg ggcccacccg
     1621 catgcccccg ccgcgccgtt cgcaatcaac cagatcgtgc acaagagcaa cgaccggctc
     1681 gagcacgaca tggaaatggt ggtcaagtac aaggtgccgg tgatcatcac ctcgctgggc
     1741 gcgcgcacgg acgtgaatgc cgccatccac agctatggcg gcatcgtgct gcacgacatc
     1801 atcaacaacg cctttgcgca caaagcggtg gagaagggcg cggacggcat catcgccgtg
     1861 gccgcgggcg cgggcgggca tgcaggcgtc aagagcccgt tcgcgctgat ccaggaaata
     1921 cggcagtggt tcgatgggcc cctcgtgctc agcggttcga tctcgaccgg cggcgccgtg
     1981 ctggccgcgc aggcaatggg tgccgacttc gcgtacatcg ggtcggcttt catcgccacg
     2041 cacgaggcgc gcgcctccga cgcctacaag caggcgatcg tcgagtgcga ttccgacgac
     2101 atcgtgtatt cgagcctgtt caccggcgtg cacggcaatt acctgaagcc ctccatccgg
     2161 gcggccggcc tggaccccga caacctcccg gacggcgatg tgaagaccat gaacttcgcc
     2221 ggcggcgaag gcagcaagtc gaaggcctgg aaggacatct ggggctgcgg ccagggcatc
     2281 ggcgccgtga ccgaggtcac ctccgcggcc gcgctggtgg cccgtctcaa gcgggaatac
     2341 cgggcggcgc aacagcgcct tctgccgcag cccgtggtgc tcgaagcggc ttagcaatat
     2401 tcacgtcata cgcggcacta gccgagccct actgccccgg gccataggtg aggctgctcg
     2461 cccggcccca gaacacgcgg tacatgaaga ccgtgtagac gatgatcgcc ggcagcgtga
     2521 tcgccacccc gacgaaaatg aagatcagcg acttggtcgc gcttgcggcc tgccagatcg
     2581 tcagccggtc gacgacgatg tacggataga ggctgtaggc gaggccaagg aacgacagca
     2641 cgaagatcag cacggtgccg gcgaagacca cccagccgta accggccgcc acgaccctgg
     2701 gcctgccggt gacgtggaag acggcgtaga acgccacggc gcacgcaacc gggatcggca
     2761 gcaggccgat gaactgcggc atgctgaacc agcggtcgaa cacggtctgg ctcaccatcg
     2821 gcgtcgccag cgaaatcccc acgagcgccg cgcccatggg ccacagcacg gtgcgcgccc
     2881 agcccacggc cttgagctgc aacgcgtcgc ctgtcttcat gatcagccat ccggcgccca
     2941 gcatggcgta ggccgtgggc agcgtcagtg cgatgcccag gctgaacagc atcggccaca
     3001 ggctccggtc gaatcccgtc aggtagctgc cgagcatcca tccctgggcc gcggcggcca
     3061 tgaacgatcc gagcgtgaac aggcggttcc aggtcgtctt ctgcgcggcg cgcgccttga
     3121 cccggaagtc gaacgagacg ccgcgcagga ccaggccgat cagcatcacg gccaccggca
     3181 ggtacagcgc cgtgagcacg atgccatgcg ccagcgggaa cgcgatcagc aacacgccga
     3241 tgcccagcac cagccaggtt tcgttggcat cccagaacgg gccgatgctg gacaccatga
     3301 cgtccttttc cccttcggtg gcgaacggca gcaggatgcc cacgcccaga tcgtagccat
     3361 cgagcaccac atacgcgagc agcgcaagcc ccatcaccgc catgaaaatc agcggcagga
     3421 ggtgcgccca gtccaggttc atcttgttct cccgtttcag gccgggccgc cgatcggcag
     3481 ggccgccttg gcgctttgca gcgtgtcggc tgtcggctgc gcgggatggc tcgccatgta
     3541 catcagcacc agcacatagg aggccagcag gaaagcgtag accacgaggt acgcgacgag
     3601 cgtggaagcc accatggcca ccggatgcgg cgccaccagg tcggctgtgc gcagcaggcc
     3661 gtacaccatg aagggctggc ggccgatctc ggtgacgaac cagcccgcca atgtcgagac
     3721 ccatccggaa aaggtcatgg ccgccagcag cagcagctgc cagcgcgaga agggcaccgc
     3781 gtcgtcgcgc gccggggcgc ccgcgcgccg gcgccacaac gtccaggcac acggccagga
     3841 caccagcagc atcagcacgc cgatgcccac catcacgcga aagctccaga acaccggggc
     3901 caccggcggg tgcgcaccgg ggaattcgtc caggcccttg agctcggctt cccaatcgtg
     3961 ggccaggatc aggctggcca gcttggggac ctgcagcgcg tagtccgtgc gccgctcttt
     4021 ctcgttgggc cagccgaaca gcgtcagcgg cgcgctcctc tccgtatgcc agatcccctc
     4081 gatcgccgcg atcttggcag gctggtgctc gagcgtattg aggccgtgca ggtcgcctgc
     4141 gaagatctgc agcggtatcg cgatcgccgc cgccaccacg ccggcgttca gcgcgcggcg
     4201 ggttccgctg ttggccgtgc cccgtagcag ctgccaagcc gacaggccgg cgatcaggaa
     4261 cgaagccgtc agtgccgaag ccagcagctt gtgcaccagc cggtacggga aggacgggtt
     4321 gaaaatgacg gccagccagc tggttgcatg gaagcggccg tcgatgattt cgtagccggc
     4381 cggcgtctgc atccaggagt tcaggctgag aatccagaac gccgacagcg tggtgccggc
     4441 ggcgaccatc agcgtcgcga acaggtggac acggtccgag acccggccgc gcccgaacag
     4501 catgatgccc aggaacgtgg cttccaggaa gaacgcggtc aagacctcgt agcccaaaag
     4561 cggtccggcg atgttgcccg cgcgttccat gtaccccggc cagttggtgc cgaactggaa
     4621 gctcatggtg atgcccgtga ccacccccag cgcgaacgtc agcgcgaaga tcttggtcca
     4681 caggtaataa gcctcctccc agccctgggc ctcggcggtg tccccggccg cgctgtggcg
     4741 cagccggaag tacaccagga accagcacag cgcgatattg atggtgggga agagaatgtg
     4801 aaagccgatg ttcgcggcga actggatgcg cgagagcagg agcgtgtcca tgcggccatt
     4861 atcggcgctg cccgccgatc gcgtgtggcc ggcgttcagg agctgtatcg ctcttcggtt
     4921 gtcatgccgg actccgatcc ggcatccatg agttcctgga ttgcgggtca agcccgcaat
     4981 gacagccctg ccggtcggct cgaccaacgc gcgtcacgcg ggttagtgca aacggtcggc
     5041 catttcccgc agttcgacgg cggcggccgc gcgctccgcg gagggcagac ggtccaggtc
     5101 gatcagcacc cgcatgccca ggcgttccca ctggaacacc ttgcgcgcgt tggatcggac
     5161 ttccgcctct tcctgcacgg cataggattc gatcggatcc gcgaagccct tcaccgtcag
     5221 gggctcctgc tcctcggcca cgatctcgtc gcgaaccagt ccataggtct ccctggagat
     5281 caggatgccg ccggggtcgg cggcctgctc cagccgggcc gccaggttca cctcggcgcc
     5341 gatgatggtg tagtccatgc gcaggtcgct gccgaagttg cctacgtcgc aatagccggt
     5401 attgatgccc atgcgaacct ggaagatctt gtccgagccc atctcgcgcc acagcatctg
     5461 cagttcggcc atgcggcgct gcatcgcaat cgccatcttc acgcattgca gcgcgtcttc
     5521 cttgatgcct ctactttccg ggtcgccgaa aaaaatcacc atcgcgtcgc cgatgaactt
     5581 gtccagcgtg ccgccgtact ccagggcgat cttggacatc tcggagaaat agctgttcag
     5641 caggaacgtg atgtcctcgg gctgccattt ggccgtggac gcggtgaagt ccttgatgtc
     5701 ggagaaaaac accgtgagct tcttgcgctg ggtccggatc tcggcgtcgc gcgagccgtc
     5761 gaacagcgac ttgtacacct gcggggcgag gtagcggccg agcttttccg acagcgactg
     5821 caggcgccgc gcgttgctgc tgcgctcaag gtgcgcgtgc acgcgcgcca gcacgacggg
     5881 cgggctgatc ggcttggtga tgtagtccac cgcgccgaga tccagcccga actgctcttc
     5941 ttcgatgctg gtcagggccg tgagaaagat gaccgggatg ccggcggtcg cggtgtcgtt
     6001 gcgcagccgg cgcatgactt cgtagccgtc catctccggc atcatgatgt ccagcaggat
     6061 cagcgcgggt ggcgcgtccg actgcgcgat ccgcagggcc tcctgcccgc ccgaggcggt
     6121 cgccacctcg tactcgtcct cgagcagggc gctcatcagg aagatgttat ccggcgtgtc
     6181 gtcgaccacc agaatcctgg gcttgcggtc ggtgggctgg gtcgtatcca tgggcgcttc
     6241 tcgatgtcgg gagttcgccg ccccaatgtt gggcgacgaa tgaatgagaa catccgcgcc
     6301 attggccctg ctacgaaaaa cactcgcaag cgctaggcaa tatgctgtcc gctgaaagtg
     6361 ttaagtgata cagcatgttc cgcaggcgtt gtgaacactg gccaatattg cgatactgtt
     6421 tatatgtaca gtctatggac aatgcctcgt ggatgaagtt cgtatccccc tggaggggct
     6481 gaaaggccgc ggcgcggcga cgcggctggc gcatcgcttc gagcgcgata cgcgcggcgc
     6541 tttcgacgat ggctgggcca cgctcgaagc agggctggca caagcgcacg aagtaccgca
     6601 caccgaggtg atctgggaag aagcgcgcag catcatgacc agcaacgaat cgcccgacgt
     6661 ttatttcgac cgctcgatca acccctaccg cggatgcgaa cacggctgca tctactgtta
     6721 tgcgcggccg acccatagct acctgaacct gtcgccgggc ctggacttcg aaacgaagat
     6781 catcgccaag cgcaacatcg cggcgctgct gcgggctgaa ctctcggcga ggaactacga
     6841 gccgcggctc attgcgatcg gcaccgccac cgattgctat cagccggttg agcgcgaact
     6901 gcggctgacg cgctccgtca tcgagctgat gcacgagacg cgccatgctt tttcgctggt
     6961 gaccaaatcg agcggcgtgg agcgcgacct ggacttgatc gcgcccatgg cggcgcaagg
     7021 gctggccgcg gtgtacctca cgatcaccac gctcgatgcc gatctcacgc gcaagctcga
     7081 gccgcgcgcg gcggcgcccc accggcgcct gcgcaccttg cgcacgctgg ccgagcatgg
     7141 cgtgccttgc ggcgtgagcc tggcgcccca gattccgttc ctcacgcagg acatggaaca
     7201 ggtgctggaa gccgcctggg acgcgggcgc gcgcagcgcc ttctaccatg tgctgcgcct
     7261 gccgtgggag gtcagtgcgc tgtttcgcca gtggctggaa ctgcattacc cgcagcgcgc
     7321 gggccgcatc atggcgcgta tccatgacat gcgcggaggc aaggactacg acagcgattt
     7381 cgccacgcgc atgaagggca cgggcccgtg ggcggaattg atcgggcagc gcttcctgaa
     7441 ggccagccgc cggctcggct tcaacagcgg ccgtgtggac ctggacacca cggcttttcg
     7501 aaagcccggc gccgcggggc agggccatct gttttagcgt cactggctcc catggcgcgg
     7561 gcgctccgaa gccggtcaga tgaagccgct gaacagaagg aagccagtga gggcgagcac
     7621 ggcgccggcc accatgacac ctgt
//
LOCUS       DASWIY010000310         7429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1698362, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000310 DASWIY010000000
VERSION     DASWIY010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698362"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..486)
                     /locus_tag="VGA59_11345"
     CDS             complement(40..486)
                     /locus_tag="VGA59_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9720317.1"
                     /translation="MNIFEALRVSHETQRALSARLLASEPGTPERHRVFLDLKRELLV
                     HETAEERCFYVPMMKHDATVDAARHAIAEHHEMDEMVEELEGQELNAPGWMAGAKKLC
                     EKVEHHLQEEETKFFQEAGKVLTEPQKLSLAKEYEAEFLTLRTKED"
     gene            complement(575..1348)
                     /locus_tag="VGA59_11350"
     CDS             complement(575..1348)
                     /locus_tag="VGA59_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720318.1"
                     /translation="MAKWTAFPQLGDFHFTNASVKKQWGRLHAGDAEPLPENPEVIEA
                     WTLFHNGEFQKAAEAGLKAGGGGITAANKATAIYANYLEKKEKTKLDLFVEVSQRAEA
                     QAAQDAKNPNAWYWQAYALGRYSQGISVAKALAQGLGGKVKEALEKTIKLQPKHADAH
                     IALGAFHAEVIDKVGSLIGGMTYGAKKEVGLKLFQDALKINPASAIGMIEYANGLVML
                     EGDRKMKEATKLYEQAAASKPMDAMERLGVELAKAELQE"
     gene            complement(1365..2030)
                     /locus_tag="VGA59_11355"
     CDS             complement(1365..2030)
                     /locus_tag="VGA59_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9720319.1"
                     /translation="MAKKAPRRTAERILEVTLVLFNRFGEPNVSTTLISAELGISPGN
                     LYYHYPAKDELINSLFDRYERSLSELLNASDGVRDVEDAWFFMHTLFELIWQYRFLYR
                     DLNDLLSKNRRLETHFQWVLKNKTRAIKTMLDSMSRSGCVSIDSREVEATATSMVVVL
                     TYWLSYEYVRDPRRALEPESAQVALLRGAHHVLNLLVPYLEQGQRMHLLGLIAAYSNS
                     ETS"
     gene            complement(2040..3290)
                     /locus_tag="VGA59_11360"
     CDS             complement(2040..3290)
                     /locus_tag="VGA59_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9720320.1"
                     /translation="MRFRKSSSPKIALALAGGGPLGAIYEIGALCALDEVLEGVDFTR
                     LHHYVGVSAGGFIAAGLANGMSPRELCASFIESDDGTSEVFDPSWLMVPAYGEFARRA
                     IMFPGLAASALWRATLGRRSLTHSLERLGPSLPTGIFSNEQVDRRLARLFSRRGRTND
                     FRELKAQLTLVATNLDTGEAAPFGRPGWDHVPISKAVQASSALPGLFPPVEIDDQYFV
                     DGALKKTLHASVALDGGVDLMLCLNPLVPFNSTEPAAAKVMQRGLPAERRRIPRIADG
                     GLPAVLSQTFRSMIHSRMELGMKHYERAYPHTDILLIEPDHRDPELYLANTFGYGRRR
                     QLAEHAYQQTRAMLRSRRGSLSAKLLRHGIRLSAQVLDDPRRHLVAPPRPLTRVGRAV
                     ASLQEVMEDLGHTVQPGLAGRSGA"
     gene            complement(3420..3917)
                     /locus_tag="VGA59_11365"
     CDS             complement(3420..3917)
                     /locus_tag="VGA59_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX9720321.1"
                     /translation="MVKKLQKKAAEQKSTGAQLSGTVKESAQQIWLAGLGAFAKAQEE
                     GGKVFEALVKEGTSIQRKTQAAAEEKLSDATNRMATMATDISSRASGQWDKLENIFEE
                     RVSKALNKLGVPSSKDIDVLISRIEELNRNVAKLSAKTTAAGRRTVNGAAKPAAKRAS
                     ARKSA"
     gene            4413..6398
                     /locus_tag="VGA59_11370"
     CDS             4413..6398
                     /locus_tag="VGA59_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9720322.1"
                     /translation="MQYFVTGATGFIGKRLVKKLLERKGSVVYFLIRKESEDKVAALR
                     EYWGSTAARTIPVFGDLTARKLGVGPEDVKKLKGQIDHFHHLAAVYDLSADEESQITV
                     NVEGTRNTVEFAKAIDAGHFHHVSSIAAAGLYEGVFREDMFDEAENYEHPYFLTKHES
                     EKIVRKDCKVPWSVYRPAMVVGDSQTGEMDKIDGPYYFFKLIQRMRQLLPPWMPAVGL
                     EGGRVNIVPVDFVVDALDYLSHKSDINKRCFHLVDPVGYRVGDVLDIFGRAAHAPKMN
                     LFVNAALFGFIPKSIKKGLMAVAPVRRIRNAVMKDLGLPEDILAFVNYPTRFDSRDTQ
                     AALKGSGIEVPNLKDYAWRLWDYWERHLDPDLHIDRTLKGTVGGKVVLVTGGSSGIGL
                     AAAHKFAEAGAITIICGRDQDRLDEACRQAKAKGYQFVAYAADIADMADCDRFTQLLV
                     KNHGGVDFLINNAGRSIRRAIEASYDRFHDFERTMQLNYFGSLRVTMGLLPAMVARHR
                     GHVVNISSIGVLTNAPRFSAYVASKAALDAWTRCASSEFADQGISFSTINMPLVRTPM
                     IAPTQLYKNVPTLAPEEAADMIAQACIFKPVRVATRLGITGQILHALLPRVAQIAMNT
                     SFRMFPDSSAAKGDKSGKPTLSAEAIAMQQMMRGIHF"
     gene            6517..7368
                     /locus_tag="VGA59_11375"
     CDS             6517..7368
                     /locus_tag="VGA59_11375"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9720323.1"
                     /translation="MDFTTRVNFLKNRLVGLLSGESSRAAALAEERDALIKERDLLAQ
                     RAKELMLCGAPSELDRTLYSIPAPPTVNPWEWLSGFANNKDSSILEIGSREVVGKSVC
                     KTRLPLAKYTGFDFHQGENVDVVGDAHRLSDYFAPNSFDVVYSAAVFEHLAMPWVVAE
                     EITKVLRVGGVACILTHFSYGEHEVPWHFFQFNSTGLEALFNESLGFKTIYAAKEMPM
                     VGRFAYDCDADHAGKAIANLYCSSYIVTEKNKDIFHGDAAVEFRWRDALDGIYGQTRY
                     PSDTSRL"
BASE COUNT         1301 a         2307 c         2375 g         1446 t
ORIGIN      
        1 catggattcc ggatcaagtc cgggatgaca ggcctgccct caatcttcct tcgtccgcag
       61 cgtcaagaac tccgcctcgt actccttggc cagcgacagc ttctgcggct cggtcagcac
      121 cttgccggct tcctggaaga acttggtctc ttcctcctgc aggtggtgct ccaccttctc
      181 gcacagtttc ttcgcgccgg ccatccagcc cggggcattg agctcctgac cttccagttc
      241 ttccaccatc tcgtccatct catggtgctc ggcgatcgcg tggcgcgccg cgtccaccgt
      301 cgcgtcgtgc ttcatcatcg ggacatagaa acaacgctct tcggccgtct cgtgcaccaa
      361 cagttctcgc ttgaggtcca gaaagacgcg atgccgctcc ggcgtgccgg gttcgctcgc
      421 aagcaagcgg gccgacaggg cccgttgcgt ttcatggctg acacgcagtg cttcgaaaat
      481 attcatgcac cgcatcgtgc ctgtgaaagc gcgcggcgtc tgtaggcgcc tggcgcgaaa
      541 cgccccggcg ccctcgaaca gcgccgccgg ctgcctactc ctgaagctcg gccttggcca
      601 gttccacgcc cagccgttcc atggcgtcca tgggtttgct ggcggcggcc tgctcataga
      661 gcttggtcgc ctctttcatc ttcctgtcgc cttccagcat gaccaggccg ttggcgtact
      721 cgatcatccc gatcgccgaa gccggattga tcttcagcgc atcctggaac agcttcaggc
      781 cgacttcctt cttcgcgcca taagtcatcc cgccgatcaa cgagccgacc ttgtcgatca
      841 cttcggcgtg gaacgcgccc agcgcgatgt gggcatcggc atgcttgggt tgcagcttga
      901 tggttttctc gagcgcttcc ttgaccttgc cgcccaggcc ctgggccagc gccttggcca
      961 cgctgatgcc ctggctgtag cggcccagcg cgtaggcttg ccagtaccag gcgttggggt
     1021 tcttcgcgtc ctgcgcggcc tgcgcttcgg cgcgctgcga cacctcgacg aacaggtcca
     1081 gcttggtctt ttcctttttc tcgagatagt tggcgtagat ggccgtggcc ttgttggccg
     1141 ctgtgatgcc gccgccaccg gccttcaggc ccgcctcggc ggctttctgg aactcgccgt
     1201 tgtgaaacaa ggtccaggcc tcgatgacct cgggattctc gggcaggggc tcggcatcgc
     1261 cggcgtgcag gcgtccccat tgcttcttca cgctggcgtt ggtgaaatgg aagtctccca
     1321 gctgggggaa agcggtccat ttggccatgg tcgtctcctt tgttttatga agtctcggaa
     1381 ttgctatagg cggcgatcag gcccagcagg tgcatgcgct ggccctgttc aaggtagggc
     1441 accagcagat taagcacatg gtgcgccccg cgcaacaagg cgacttgcgc gctttcgggt
     1501 tccagcgcgc ggcgcgggtc gcgcacgtat tcatagctca gccagtaggt cagcaccacc
     1561 accatgctgg tggccgtggc ctcgacttcg cgtgaatcga tgctgacgca gcccgaccgg
     1621 ctcatgctgt cgagcatggt cttgatcgcg cgggtcttgt tcttcagcac ccactggaag
     1681 tgcgtttcca gccggcggtt cttcgacagc aggtcgttca ggtcgcggta gaggaatcgg
     1741 tattgccaga tcagctcgaa cagcgtgtgc atgaagaacc acgcgtcctc cacgtcgcgc
     1801 acgccgtcgc tggcgttcag cagctcgctc agggaccgct cgtagcggtc gaacagcgaa
     1861 ttgatgagct cgtccttggc cgggtagtgg taatagaggt tgcccgggct gatgccgagt
     1921 tcggccgaga tcaatgtggt cgagacgttc ggctcgccga agcggttgaa caggacgagt
     1981 gtgacctcga ggatgcgttc ggccgtgcgg cgaggggctt tcttggccat ggccggtgat
     2041 caggccccgc tgcggccggc gaggcccggc tggacggtgt ggcccaggtc ttccatcact
     2101 tcctgcagcg acgcgaccgc gcggcccacg cgtgtgagcg gtctgggcgg ggccaccaga
     2161 tgccggcgcg gatcgtccag tacctgcgcg cttagccgta tgccgtgccg cagcagcttg
     2221 gccgagaggc tgccgcggcg cgatcgcagc atggcacgcg tctgctggta ggcatgttcg
     2281 gccaattgcc ggcgccggcc atagccgaag gtgttcgcca gatagagttc ggggtcgcgg
     2341 tggtcgggct cgatcaggag gatgtcggtg tgggggtagg cccgttcgta atgcttcatt
     2401 cccagttcca tgcgggaatg gatcatcgat cggaaggtct ggctcagcac agccggcagg
     2461 ccgccgtcgg caatgcgcgg aatgcgtcgc cgctcggccg gcagcccgcg ctgcatgacc
     2521 ttggcggcgg ccggctcggt ggaattgaag ggcaccagag ggttgaggca cagcatcagg
     2581 tccacgccgc cgtccagggc gaccgaggcg tgcagggtct tcttcagcgc gccgtcgacg
     2641 aaatattggt cgtcgatctc caccggcggg aacagcccgg gcagcgccga actcgcctgc
     2701 accgctttgg agatcggcac atggtcccac cccggccggc cgaagggcgc ggcttccccg
     2761 gtatccagat tggtcgccac cagcgtgagc tgcgccttga gctcgcggaa atcgttggtc
     2821 ctgccccggc gcgagaacag ccgtgccagc cgccggtcga cctgctcgtt ggaaaagatg
     2881 ccggtgggca ggctgggacc cagccgttcg agcgagtggg tcagcgatct gcggcccaat
     2941 gtggcacgcc acaacgccga tgccgccagc cccgggaaca tgatcgcccg gcgcgcgaat
     3001 tcgccatacg caggcaccat gagccaggaa ggatcgaaca cttcgctggt gccatcgtcg
     3061 ctttcgatga acgaagcgca cagttcgcgt ggcgacatgc cgttagccag gccggcggcg
     3121 atgaagcccc cggccgaaac gccgacgtag tggtgcaggc gcgtgaagtc aactccctcg
     3181 agcacttcgt ccagtgcgca caaggccccg atctcgtaaa tcgcgcccaa tgggccgcct
     3241 ccggcgagcg caagcgcgat tttggggctg gacgattttc tgaatctcat gtgcggtcag
     3301 tgtagggcgc ttgcctcacg ttctctgctg cgttgcagca aaacaggctg gccttcgcaa
     3361 caggtgccgg ggcgacaaaa aaggcgccga agcgccttgg gtgagtgccc ggcctcgcgt
     3421 caggccgact tgcgcgcgga ggcgcgcttg gcggcgggtt tggcggcgcc gttcactgta
     3481 cggcgaccgg ccgccgtggt tttcgcgctc agtttggcga cattacggtt gagctcctcg
     3541 atgcgcgaga tcagcacgtc gatgtccttg gacgagggaa cgcccaactt gttgagggcc
     3601 ttggacacgc gttcctcgaa aatgttttcc agcttgtccc actggcccga agcgcgcgac
     3661 gagatgtcgg tcgccatcgt ggccatgcgg ttggtggcgt cggaaagctt ttcctcggcg
     3721 gccgcctggg tcttgcgctg gatcgaggtg ccttccttga ccagggcctc gaacaccttg
     3781 ccgccttctt cctgcgcctt cgcgaaagcg cccaggccgg cgagccagat ttgctgtgcc
     3841 gattccttga cggtgccgga cagttgagcg ccggtcgatt tttgttcagc agccttcttt
     3901 tgcagtttct tgaccatggt ggcctccagt cggtgtgttt agggttccgc gccgccggtc
     3961 tcacccggca cggcgcacag atggacttta agccttgggg acgggtgctt tctaggtgcg
     4021 gctttctaaa gcgctaggcg atcaacccta gcagttctgt cattgcgggc tcgacccgca
     4081 atccaacggg gctgtcattg cgggctgggc tcgcggaacg cccggggcgc gcagctgtcc
     4141 agtgcagcga gcccaatgcc gggcctgcgc tatgcacgta ccgaggcatc acgcgacggc
     4201 ccggccccgc aatccaggaa gtcatggatc ccggatcgag tccgggatga cagaaagact
     4261 cgtccgggat gacaagaccg cgtggtttag ttgcggcttc ctaaagcgct agacaatcga
     4321 ccctaggcag cgggcgggtt tgtgctgaat cgccgccgcg ccgctttggg caagaatccc
     4381 cccaaagcaa aatccaatcc tccggaggtt caatgcagta tttcgtcacc ggcgcgaccg
     4441 gcttcatcgg caagcgcctg gtcaagaagt tgctcgagcg caagggctcg gtcgtgtatt
     4501 tcctgatccg caaggaaagc gaggacaagg tcgcggcgtt gcgcgagtac tggggctcga
     4561 ccgccgcccg cactattccc gtgttcggcg atctcaccgc caggaagctg ggcgtcgggc
     4621 ccgaggacgt caagaagctc aagggccaga tcgaccattt ccaccacctc gccgcggtat
     4681 acgacctgtc cgccgacgag gagagccaga tcaccgtcaa cgtcgagggc acgcgcaaca
     4741 cggtggaatt cgccaaggcc atcgatgccg ggcactttca ccacgtgtcc tcgatcgccg
     4801 ccgcgggatt gtacgaaggc gtgtttcgcg aggacatgtt cgacgaggcc gagaactacg
     4861 agcatcccta tttcctgacc aagcacgaga gcgagaagat cgtgcgcaag gactgcaagg
     4921 tgccctggtc ggtctaccgg ccggcgatgg tcgtgggcga cagccagacc ggcgagatgg
     4981 acaagatcga cggcccctac tacttcttca agctgatcca gcgcatgcgc cagctgctgc
     5041 cgccctggat gccggcggtg ggcctggaag gcgggcgcgt caacatcgtg ccggtggact
     5101 tcgtggtcga tgcactggac tatctgagcc acaagagcga catcaacaag cgttgcttcc
     5161 atcttgtcga ccccgtcggg taccgggtgg gcgacgtgct ggacatcttc ggccgtgccg
     5221 cgcatgcgcc caagatgaac ctgttcgtca acgccgccct gttcgggttc attcccaaga
     5281 gcatcaagaa gggcctgatg gcggtggcgc cggtgcgccg catccgcaac gccgtcatga
     5341 aggacctggg gctgccggag gacattctgg cgttcgtcaa ctatcccacc cgcttcgaca
     5401 gccgcgacac gcaggcggcg ctgaaaggct cgggcatcga agtgcccaac ctgaaggact
     5461 acgcctggcg actgtgggac tactgggagc gccacctcga ccccgacctg cacatcgacc
     5521 gcacgctcaa aggcacggtg gggggcaagg tggtgctggt caccggcggc tcctcgggca
     5581 tcgggctggc ggcggcgcac aagttcgccg aggcgggcgc gatcaccatc atctgcggcc
     5641 gcgaccagga caggctggac gaggcgtgca ggcaagccaa ggccaagggc taccagttcg
     5701 tcgcctacgc ggcggacatc gccgacatgg ccgactgcga ccgtttcacc cagctgctgg
     5761 tgaaaaacca cggcggcgtg gacttcctga tcaacaacgc cgggcgttcg atccgccgcg
     5821 caatcgaagc cagctacgac cgcttccacg atttcgagcg caccatgcag ctcaactatt
     5881 tcggcagcct gcgcgtgacg atgggcctgc tgccggccat ggtggcccgg cacaggggcc
     5941 acgtggtcaa catcagctcg atcggcgtgc tgaccaatgc gccgcgcttt tcggcctacg
     6001 tggcctcgaa ggcggcgctc gatgcgtgga cacgctgcgc ctccagtgag ttcgcggacc
     6061 aggggatcag cttctcgacg atcaacatgc cgctggtgcg caccccgatg atcgcgccga
     6121 cccagcttta caagaacgtg ccgacgctgg cgccggaaga agcggccgac atgatcgcgc
     6181 aggcctgcat cttcaagccg gtgcgggtcg ccacgcggct gggcatcacg ggccagatcc
     6241 tgcatgcgtt gctgccgcgc gtggcccaga tcgcgatgaa cacgagcttc cgcatgttcc
     6301 ccgattcatc cgccgccaag ggcgacaaga gcggcaagcc gacgctgtcg gccgaggcga
     6361 tcgcgatgca gcagatgatg cgggggattc acttctaggc gcgccgggag ctgggcgggc
     6421 cggccgattt gccgcggcaa gggcagccgc ggcgcgtgac gctttgatgg gttgatcccc
     6481 actctctcat tcgaatcaga cagacccgga cgtatcatgg atttcacaac gcgggtcaat
     6541 ttcctgaaga atcgtctggt cgggttattg agcggcgaat caagcagggc cgcggctctt
     6601 gctgaggaac gggatgctct gatcaaggag agagatttgt tagctcagcg ggcaaaggag
     6661 ttgatgcttt gcggcgcgcc ttctgaactt gacagaaccc tgtattcgat tccggctccc
     6721 ccgacagtca atccttggga atggctctca ggcttcgcca ataacaagga ttcttccatt
     6781 ctggagattg gttccagaga ggtggtcggc aagagcgtct gcaagacgcg cttgccgctg
     6841 gcgaaataca caggctttga cttccaccag ggagaaaacg tcgacgtggt cggtgacgcc
     6901 catcgcctgt ccgactactt tgcaccgaat agttttgatg tggtctattc ggctgccgtg
     6961 ttcgagcatc ttgcaatgcc gtgggtggtc gctgaggaaa ttacaaaagt acttcgtgtg
     7021 ggcggtgtgg cctgtatcct cacgcatttc agctatggcg agcatgaggt gccttggcat
     7081 ttctttcagt tcaacagcac gggactcgag gcgcttttca atgaaagcct cggattcaag
     7141 accatttacg cggccaagga aatgcccatg gtgggcaggt ttgcctacga ttgcgatgca
     7201 gaccatgcgg gaaaggcaat cgcaaatctc tactgttcct cctacatcgt gacagaaaag
     7261 aacaaagaca tatttcacgg cgacgcagca gtcgaattcc ggtggagaga tgctcttgat
     7321 gggatatatg ggcaaactcg atatccgagt gatacaagcc ggctttgacg ccgttgggag
     7381 cgaccagagg ggcaagccgt cggcttgagc cggtccggcg accccgatg
//
LOCUS       DASWIY010000311         5096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_200983, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000311 DASWIY010000000
VERSION     DASWIY010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200983"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VGA59_11380"
     CDS             <1..303
                     /locus_tag="VGA59_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX9720324.1"
                     /translation="SAENANRQKAELRQIGVSFEALADPSLEPGLSLGGFATEAAATQ
                     AMEALSQRGVRNAQVLRERAELRGLLLKLPAVDDSLRPRLGELASVLGGRNLRPCR"
     gene            complement(362..1840)
                     /locus_tag="VGA59_11385"
     CDS             complement(362..1840)
                     /locus_tag="VGA59_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9720325.1"
                     /translation="MKIFPREQRSLFGEILDWMLTPLLLLWPVSLALTWLVAQNIAGK
                     PFDRALEYNVQALAQLVIVQQQRAVFNLPQPAREILRADDSDLVYYQVLGAHGELLSG
                     ERDFPLPDEDERPLIGEVRMRDDEMRGQDVRVAYTWVKLDLPGAQPSLVQVAETREKR
                     SVLATEIIKGVMLPQFVILPLAVLLVWLALVRGIKPLSQLEERIRARKPDDLSPLDDK
                     TVPLEVAPLVSSVNDLLTRLKDSIATQKRFLADAAHQLKTPLAGLRMQADLAQREAFN
                     AEELKQSLKQIGRSSIRATHTVNQLLALARAESSGHTIARQPCDLAQLTMEAVQDSVP
                     RTLDKHIDLGYDGAQPGSPGVWLSGNPTLLQEMIRNLLDNAINYTPSSAEHPGVITAR
                     VLADPFGHVLILQVEDSGPGIPESERELVLQPFYRALGTNVDGSGLGLPIVLEIARQH
                     NAEIKIEDAHPGRVPPGSRVSVRFVLGDEAAGVEAAEAQVPL"
     gene            complement(1985..2659)
                     /locus_tag="VGA59_11390"
     CDS             complement(1985..2659)
                     /locus_tag="VGA59_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9720326.1"
                     /translation="MRILIAEDDQVLADGLLRTLRASGAAVDHVASGTEADAALMTNN
                     EFDLLILDLGMPKMHGLEVLRKLRGRGSSLPVLILTAADAVEERVKGLDYGADDYMAK
                     PFSLQELEARVRALTRRGMGGTSSVIKHGPLVYDQAGRVATIDGKMIELSARELGLLE
                     VLLQRAGRLVSKDQLVERLCEWGEEVSNNAIEVYIHRLRKKIEKGPIRIATVRGLGYC
                     LEKIPG"
     gene            complement(2720..3205)
                     /locus_tag="VGA59_11395"
     CDS             complement(2720..3205)
                     /locus_tag="VGA59_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX9720327.1"
                     /translation="MDKRPPVIDDGWRLTHLGRLLGHAMRRFDERVLQLMARNVEVPL
                     ALSNLAARSQVSAAHIHITRHLTLDGSRLTDLAQRAGMSKQAMGDLVDQCEAWGLVTR
                     EPDSQDARARLVRFTPTGLAWLQAFKEAVAQAEAEFRADVGAEVATVVMIGLEAYAGA
                     A"
     gene            3348..4421
                     /gene="recA"
                     /locus_tag="VGA59_11400"
     CDS             3348..4421
                     /gene="recA"
                     /locus_tag="VGA59_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX9720328.1"
                     /translation="MDAQVKGTRIAADSEKARALQAALAQIDKQFGKGTIMRLGEGEK
                     LEDIQVVSTGSLGLDIALGVGGLPRGRVIEIYGPESSGKTTLTLQVIAEMQRQAGQCA
                     FIDAEHALDVQYAQKLGVNLSDLLISQPDTGEQALEIVDSLVRSGAVDLIVIDSVAAL
                     TPKAEIEGEMGDSLPGLQARLMSQALRKLTASIKRTNCMVIFINQIRMKIGVMFGSPE
                     TTTGGNALKFYASVRLDIRRTGTIKKGEEAIGNETKVKVVKNKVAPPFKTAEFDILFG
                     EGISRHGEIIDMGVNARILEKSGAWYAYNGEKIGQGRDNAREFLRENPDLAREIENKV
                     RESLGIPLLPPDVEEEAPAAKSK"
     gene            4426..4869
                     /gene="recX"
                     /locus_tag="VGA59_11405"
     CDS             4426..4869
                     /gene="recX"
                     /locus_tag="VGA59_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEX9720329.1"
                     /translation="MTRGQLSLKGRALRLLSGREHSRVELERKLAAREEEPGQLRRVL
                     DELQAKGFIDEQRVLESVLHRRAARLGAARIRQELQAKGLDPQMVARAVESLRSSELQ
                     RAREVWRKKFGEPPADARERGQQARFLAARGFDGEVIVRVLAGAE"
BASE COUNT          858 a         1711 c         1717 g          810 t
ORIGIN      
        1 agtgccgaaa acgcaaaccg gcaaaaggcc gagctgcggc agatcggcgt ttccttcgaa
       61 gccttggccg acccttcgct ggaacctggc ctgtcgctgg gcggcttcgc aaccgaggcg
      121 gccgccacgc aggcgatgga agcgctgtcg cagcgcggcg tgcgcaacgc ccaggtgttg
      181 cgcgagcggg ccgagctgcg ggggctgctg ctcaagctgc cggccgtaga cgacagcctg
      241 cggccgcgcc tgggagaact cgcatcggtc ctgggcggaa gaaatctgcg gccctgccgc
      301 taggaacccg cggccctgcc gccgattcgg gcgatcgcgc gcgaacggct ccagtgaacg
      361 tctacaaggg aacctgagcc tcggcggctt cgacgccggc ggcttcgtcg cccagcacga
      421 agcgcacgct gacccggctg cccggcggca ctcggccggg gtgcgcgtcc tcgatcttga
      481 tttcggcgtt gtgctggcgt gcgatctcca gcacgatcgg caggcccagg cccgagccgt
      541 ccacattggt tcccagcgcc cggtagaacg gctgcagcac cagttcgcgc tcgctctccg
      601 ggatgcccgg gcccgagtcc tcgacctgga gtatcagcac gtggccgaac ggatcggcca
      661 gcacccgcgc cgtgatgacc ccgggatgct cggcgctcga gggcgtgtag ttgatggcgt
      721 tgtccagcag gttgcggatc atctcctgca gcagcgtcgg gttgccggac agccatacgc
      781 ccggcgaacc cggctgggcg ccgtcgtacc cgaggtcgat gtgcttgtcc agggtgcgcg
      841 gcaccgagtc ctgcacggcc tccatcgtca gttgcgccag gtcgcagggc tggcgcgcga
      901 tggtgtggcc gctgctttcc gcgcgcgcca gggccagcag ctggttcacg gtgtgggtgg
      961 cgcggatgct ggagcgcccg atctgcttga gcgactgctt cagctcctcg gcgttgaacg
     1021 cctcgcgctg ggccagatcg gcctgcatgc gcagtcccgc cagcggcgtc ttgagctggt
     1081 gcgcggcatc ggccagaaag cgtttctggg ttgcgatgga gtccttcagg cgcgtcagca
     1141 gatcgttgac ggaagacacc agcggcgcca cttccagcgg cacggtcttg tcgtccaggg
     1201 gactcaagtc gtcgggcttg cgcgcgcgga tgcgctcttc cagctgggac agcggcttga
     1261 tgccgcgcac cagggccagc cagaccagca gcacggccag cggcaggatg acgaactgcg
     1321 gcagcatcac gcccttgatg atctcggtgg ccagcaccga gcgcttttcg cgcgtctcgg
     1381 ccacctgcac gagggagggc tgcgcgccgg gcaggtcgag cttgacccag gtataggcca
     1441 cgcgcacgtc ctggccgcgc atctcgtcgt cgcgcatccg cacctcgccg atcagcggcc
     1501 gttcgtcctc gtccggcagc ggaaagtccc gctcgccact gagcagctcg ccgtgggcgc
     1561 ccagcacctg gtaatagacc aggtcggaat cgtcggcgcg caggatttcg cgcgccggct
     1621 gcgggaggtt gaagaccgct cgctgctgct gcacgattac cagctgcgcc agcgcctgga
     1681 cgttgtactc cagcgcgcgg tcgaacggct tgccggcaat gttctgcgcc accagccagg
     1741 tcagcgccag gctgaccggc cacagcagca gcagcggcgt gagcatccag tcgaggattt
     1801 cgccgaacag cgagcgctgc tcacgcggga agatcttcaa gcgcgccacg gttgtcccgg
     1861 tgggcgatgc ggccgccgcg caggcgcggt cgggggttca tgcgcgccgc cgggccgcct
     1921 caaagcgcga gcgccccctt ggggacggcg caggcatgca gtggcacgcg tgggggccat
     1981 ggatctaccc cgggatcttc tccaggcagt agccgagacc gcgcaccgtg gcgatgcgga
     2041 tcggaccctt ttcgatcttc ttgcgcagcc gatggatgta gacctcgatc gcgttgttgc
     2101 tgacttcctc gccccactcg cacagccgct cgaccagctg gtccttgctg accagacgcc
     2161 cggcgcgctg cagcagcacc tccagcaggc ccagctcgcg cgccgacagt tcgatcatct
     2221 tgccgtcgat cgtggcgaca cggccggcct gatcgtagac cagcggtccg tgcttgatga
     2281 cgctgctggt gccgcccatg ccccggcggg tgagcgcgcg tacccgggct tccagttcct
     2341 gcaggctgaa gggcttggcc atgtagtcgt cggccccata gtccaggccc ttgacgcgtt
     2401 cttccacggc gtccgccgcc gtgaggatca gcaccggcag cgaggagccg cgcccgcgca
     2461 gtttgcgcag cacttccagg ccgtgcatct tgggcatgcc caggtccagg atcagcaagt
     2521 cgaactcgtt gttggtcatc agcgccgcgt cggcctccgt gccgctggcg acgtggtcga
     2581 ccgcggcccc cgaggcccgc aaagtgcgca acagcccgtc ggccagcacc tggtcgtctt
     2641 cggcaatcag aatccgcatg gcttgtctcc ttatcggcgc cgacgtccta tgcgcccttg
     2701 gcaaattcta ggctgccgtt cacgcggcac cagcataagc ctccagcccg atcatcacca
     2761 cggtggcaac ctccgccccg acgtcggcgc ggaattccgc ctcggcctgc gccactgctt
     2821 ccttgaacgc ctgcagccag gccagcccgg tgggcgtgaa acggaccagg cgggcacggg
     2881 cgtcctgcga atcgggctcg cgcgtcacca gcccccaggc ctcgcactgg tcgaccaggt
     2941 cgcccatggc ctgcttgctc atcccggctc gctgggccag gtcggtcagg cgcgatccgt
     3001 ccagcgtcag gtggcgggtg atgtggatgt gggcggcgct gacctgcgag cgtgcggcca
     3061 ggttcgacag cgcgagcggc acctcgacgt tgcgggccat gagctgcagc acccgctcgt
     3121 cgaatcggcg catcgcatga cccagcagcc gtcccagatg ggtgaggcgc cagccgtcgt
     3181 cgatgaccgg gggccgtttg tccatcctta gatcgtaagg caaactgatg aaaaatctcg
     3241 tttactaaat tcaacgcggc acgttaaagt actggtcagg catccaggct gtgaataaga
     3301 acactggatg agacacctca aaacgttgat tcacaaggag atttctgatg gatgctcaag
     3361 tcaagggaac ccgtattgcc gccgacagcg aaaaagccag ggccttgcag gccgcgctgg
     3421 cccagatcga caagcagttc ggcaagggca ccatcatgcg cctgggtgag ggcgagaagc
     3481 tcgaggacat ccaggtcgta tcgacgggtt cgctgggcct ggacatcgcc ttgggcgtgg
     3541 gcggcctgcc gcgcggacgg gtgatcgaga tttacggccc ggagtcttcc ggcaagacca
     3601 cgctgacgct gcaggtcatc gccgaaatgc agcgccaggc cggccagtgc gccttcatcg
     3661 atgccgagca cgcgctggac gtgcaatacg cgcaaaagct cggcgtcaac ctgtccgacc
     3721 tgctgatcag ccagcccgac accggcgagc aagccctgga aatcgtcgac agcctggtgc
     3781 gctcgggcgc ggtggacctg atcgtgatcg actcggtggc cgcgctcacg cccaaggccg
     3841 aaatcgaagg cgagatgggc gactcgctgc ccggcctgca agcccgcctg atgagccagg
     3901 cgctgcgcaa gctcaccgcc tcgatcaagc ggaccaactg catggtcatc ttcatcaacc
     3961 agatccgcat gaagatcggc gtgatgttcg gcagccccga gaccaccacc ggcggcaacg
     4021 cgctgaagtt ctacgcgtcg gtgcgcctgg acatccgccg caccggcacc atcaagaagg
     4081 gtgaagaggc gatcggcaac gagaccaagg tcaaggtggt gaagaacaag gtggcgccac
     4141 ccttcaagac cgccgaattc gacatcctgt tcggcgaggg catcagccgg catggcgaga
     4201 tcatcgacat gggcgtgaat gcccgcatcc tcgagaagtc cggcgcctgg tacgcctaca
     4261 acggcgaaaa gatcggccag ggccgcgaca acgcgcgcga gttcctgcgc gagaaccccg
     4321 acttggcgcg cgagatcgag aacaaggtgc gcgaatcgct gggcattccg ctcctgccac
     4381 cggacgtcga ggaagaggcg cccgccgcca agtcgaagta aggccgtgac caggggccag
     4441 ctctcgctca agggccgggc cctgcgcctg ctcagcgggc gcgagcattc acgcgtggag
     4501 ctggagcgaa aactggctgc gcgcgaggaa gagcccggcc agctgcgccg ggtgctcgac
     4561 gaactccagg ccaagggctt catcgacgag cagcgcgtgc tcgaatcggt gctgcaccgc
     4621 cgcgcggcgc ggcttggcgc cgcgcggatc cgccaggaat tgcaggccaa gggcctggat
     4681 ccgcagatgg tggcccgtgc ggtggaaagc ctgcgatcct ccgagttgca gcgtgcccgc
     4741 gaggtctggc gcaagaagtt cggtgaaccg cctgccgatg cccgcgaaag aggacagcag
     4801 gcgcgcttcc tggcggcgcg gggcttcgat ggcgaagtga tcgtgcgggt gctggctggc
     4861 gcggaatagc cgtcattgcg ggctgggctc gcggaacgcc cggggcgcgc agctgtccag
     4921 tgcagcgagc ccaatgccgg gcctgcgcta tgcacgtgcc gaggcatcac gctccggccc
     4981 ggccccgcaa tccatgactc ctgaattcct ggatcccggg tcaagcccgg gatgacaacc
     5041 ccatcacaaa tccagcatca atttgaggtt ctgcacggcg gcgccgctgg cgccct
//
LOCUS       DASWIY010000312         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3519286, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000312 DASWIY010000000
VERSION     DASWIY010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3519286"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGA59_11410"
     CDS             <1..289
                     /locus_tag="VGA59_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX9720330.1"
                     /translation="KEEFSANFLHKLAAIAAYPALRNLGKRIDPRRHNGASFLGLNGI
                     VIKSHGSADALGFANAIRVGLEEVEKNVPARINALMAAMPAAPLSPKAAAN"
     gene            662..1606
                     /locus_tag="VGA59_11415"
     CDS             662..1606
                     /locus_tag="VGA59_11415"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9720331.1"
                     /translation="MQLPKDVGVDVAKDFIVAAFADQSSSPRQVPNQARALRAWLRTL
                     PAGSRIAMEATGRYHVLLADLAHEAGFTAYVLNPKDVWYYAKGLGRRGKTDRIDAEVI
                     ASYVADHHPKLKPYTPISPEQRAIDRLLKRRARLIVLQGAARATTGDLTECRRQVKAL
                     LDRFDSLLAEIDRQLQDLTRPLEPLRQSQARLQKIVGIGPLTGLSLASTFLRIPFRNG
                     DAVVAYSGFDPRPHDSGRMVGRRRLSKRGPSELRRLLYVAAMSAAQTPIWKPIYERYR
                     ARGLSSTASYVILARRILRVAWSVHSHQTDFDPARLAA"
BASE COUNT          313 a          573 c          556 g          304 t
ORIGIN      
        1 caaggaagaa ttctccgcga acttcctgca caagctcgcg gcgatcgcgg cctatccggc
       61 cctcaggaac ctcggcaagc gcatcgaccc ccggcgccac aacggcgcca gcttcctcgg
      121 cctcaacggc atcgtcatca agagccacgg cagcgccgac gccctcggct tcgccaacgc
      181 gatccgcgtg gggctggagg aagtcgagaa gaacgtgcca gcgcggatca acgcgctgat
      241 ggcggcgatg ccggcggcgc cgttgtcacc aaaggcagcc gccaactaag cgggcttggg
      301 ctttacgtcg gttcgcttcg gcgaggggcg ggtcggggac ttgcctccgc gaattgctcg
      361 ccggactgcg gcttgggtct ggcgggccat ggcccgccag accacggcgc cgcacccctc
      421 gggctgcgcc cgaggggctt gtatggtcgc gcggcgtctt tgagcgatgc ttgaaggcgc
      481 tggggtggag ggacgcgaca ggggctatct gccaatgact tggacagacc accggagaaa
      541 tcccccaccg catttgagcg tcgatgagga atctagcggg tatgaccgcc gatttatcgc
      601 aagctaacgc cgatgcggct tccaccccag caagcccaaa gctacgccgg gaggaaggcc
      661 gatgcaatta cccaaggatg ttggagtgga tgtcgccaag gacttcatcg tggcggcgtt
      721 tgcagatcag agcagctcgc ccagacaggt cccgaatcag gctcgcgcgt tgcgtgcctg
      781 gctcaggact ttgcctgcag gaagccgcat cgcgatggaa gccaccggcc gctatcacgt
      841 cctgctggcg gacttggcac acgaggcggg ctttacggcc tacgtgctca atccgaaaga
      901 cgtctggtat tacgccaagg gcctgggccg tcgaggcaag accgaccgga tcgatgcaga
      961 ggtcatcgca tcctatgtgg ccgatcatca ccccaagctc aagccctaca cgcccatctc
     1021 acccgagcag cgcgccatcg accggctgct caagcggcgt gcgcgactga tcgtcctgca
     1081 gggcgccgcg cgcgccacga cgggcgatct tacggaatgc cgcaggcagg ttaaggcgct
     1141 gctggaccgc ttcgattcgc tgcttgccga gatcgatcgc cagctgcaag acctgacacg
     1201 gccgctcgag ccgctgcggc agtcccaggc gcgactccag aagattgtcg gcattgggcc
     1261 gctgacgggc cttagcctgg cgagcacgtt tctgcgcatt ccgtttcgga atggcgacgc
     1321 cgtggtcgcg tattcggggt ttgatccacg gccccacgat tccggccgca tggtcggacg
     1381 ccggcgcttg tccaaacgtg gccccagcga actgcggcgc ctgctgtacg tcgccgccat
     1441 gtcagccgcc caaactccta tttggaagcc catctacgag cgataccggg cccgagggct
     1501 atcgtcgacc gccagctacg tcatcctggc gcgccggatc ttgcgggtgg cgtggtcagt
     1561 gcattcgcac cagaccgact ttgaccccgc tcgactggcc gcttgatttc gtccatagaa
     1621 tctccggtcc gtctgcgctt gtatgttcca taccggcagg tgaaaaagag cctgtcgggg
     1681 tggagggact tctgcgctat ctggccgtcc ggcacagacg agtaaagaga tcgtcccgcc
     1741 cctcac
//
LOCUS       DASWIY010000313        12744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3181295, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000313 DASWIY010000000
VERSION     DASWIY010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 12744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12744
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3181295"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(392..1489)
                     /gene="zapE"
                     /locus_tag="VGA59_11420"
     CDS             complement(392..1489)
                     /gene="zapE"
                     /locus_tag="VGA59_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEX9720332.1"
                     /translation="MSVKEVYEAELTARGYRSDPAQLRAVEALDRCAREWAQFKEQRS
                     NVWKKLIKRPDIPRGVYMYGGVGRGKSFLMDCFYTAVPIRRKTRLHFHEFMREVHREM
                     GDLQGTVNPLDELGERIAKRHRLICFDEFHVADITDAMILHRLLNALFDNGVGFVTTS
                     NFKPHDLYPDGLHRERILPAIDLLDRKLEVIGVDAGLDYRRRALEQVELYHVPLDPAA
                     DEQMNRAFTRLAESHEEDPVLHIEAREIRARRKAGGVVWFDFKTLCGGPRSQNDYLEI
                     AAQFHTVLLSGVRQMPMRMSSEARRFTWLVDVLYDRRVKLILSAEVPPEALYIEGPLA
                     HEFPRTVSRLHEMQSAGFLALEHRTVDTRLT"
     gene            complement(1623..3050)
                     /gene="lpdA"
                     /locus_tag="VGA59_11425"
     CDS             complement(1623..3050)
                     /gene="lpdA"
                     /locus_tag="VGA59_11425"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX9720333.1"
                     /translation="MNKQFDVIVIGGGPGGYIAAIRAAQLGFNTACIDEWKNSKGGPA
                     LGGTCTNIGCIPSKALLQSSEHYEHAGKHFADHGIEVKGLGLDLAKMLARKDQVVKQN
                     NDGIAFLFKKNKVSFFHGRGSFVKAGEGGYEIKMTAAAEETIVGKHIVVATGSNARAL
                     PGAPFDEENILSNDGALRVQGVPKKMGLIGAGVIGLEMGSVWRRLGSEVTVLEALPAF
                     LGLVDEQIAREARKAFDKQGLKIELGVKVGEVRNGKKGVSVAWTNAKGEARTLEVDKL
                     IVSIGRAPNTTGLNSDAVGLKLDERGAVIVDDDCRTNLPNVWAIGDVVRGPMLAHKAE
                     EEGVAVAERIAGQHGHVNFNTIPWVIYTSPEIAWVGQTEQQLKAAGVKYRAGTFPFLA
                     NGRARALGDTTGMVKFLADAASDEILGVHIVGPMASELISEAVVAMEFRASSEDIARI
                     CHAHPSLSEATKEAALAVDKRTLNF"
     gene            complement(3188..4453)
                     /gene="odhB"
                     /locus_tag="VGA59_11430"
     CDS             complement(3188..4453)
                     /gene="odhB"
                     /locus_tag="VGA59_11430"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX9720334.1"
                     /translation="MAIVEVKVPQLSESVAEATLLQWKKQAGELVALDEIVIEIETDK
                     VVLEVPAPAAGVLAQIVVPDGSTVTAEQVIAKIDTEGKAAAGAPAAEAAPTAAAPAAV
                     PPAAAPLAAPGSKAGIAMPAAAKIMADNKLAAGSVPGTGRDGRVTKGDVLGAVAGKAV
                     AAPATSPAAAAKPVLPQVQAPVAAPDLGERPEQRVPMSRLRARVAERLLQSQSSSAIL
                     TTFNEVNMAPVMEMRKKFQEKFEKEHGVKIGFMSFFVKAAVHALKKYPVINASVDGND
                     IVYHGYFDIGIAVGSPRGLVVPILRNADQMSFADIEKKIAEYGVKARDGKLGLEEMTG
                     GTFSISNGGVFGSMLSTPIINPPQSAILGVHATKDRAVVENGQVVVRPMNYLAMSYDH
                     RIIDGREAVLGLVAMKEALEEPARLLFDI"
     gene            complement(4529..7396)
                     /locus_tag="VGA59_11435"
     CDS             complement(4529..7396)
                     /locus_tag="VGA59_11435"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX9720335.1"
                     /translation="MSETSSVYTAYQGNSYLFGGNAPYVEEMYENYLANPTSVPETWR
                     DYFDALQNVPAADGSNAKDVPHMPVINAFAERAKQGGIKLVPGSGEAAMGRKRTAVQQ
                     LIAAYRNVGSRWADLDPLKRAEREKIPELDPAFYGFSDADQELVFNISNTFFGKESMS
                     LRELINALRETYCGAIGAEYMYISDQYKKRWWQQQLESIRSKPNFSIEKKKHILERVT
                     AAEGLERFLHTKYVGQKRFSLEGGESFIASMDELIQEAGAKGVQEIVIGMAHRGRLNV
                     LVNSLGKMPADLFAEFDHTAKEELPSGDVKYHQGFSSDVTTRGGPVHLSLAFNPSHLE
                     IVNPVVEGSVRARMDRRADPQGKQVLPVQVHGDAAFAGQGVVMETLALAETRGYFTGG
                     TVHIVINNQIGFTTSDPRDSRSTLYCTDVVKMIEAPVLHVNGDDPEAVVLATQLGLEY
                     RMEFAKDVVVDIVCFRKLGHNEQDTPSLTQPLMYKKIAAHPGTRKLYADKLAAQGLGE
                     TLGDDMVKGYRAAMDAGKHTTDPVLTNFKSKYAVDWTPFLRKKWTDSADTAIPMAEWK
                     RLAGKITTIPANFTAHPLVKKVLDDRAAMGRGETGVDWGMGEHMAFASLVASGYPVRL
                     SGEDSARGTFSHRHAVLHDQKREKWDTGTYTPLENVAENQAPFVIIDSILSEEAVLGF
                     EYGYASNDPNTLVIWEAQFGDFVNGAQVVIDQFIASGEVKWGRINGITLMLPHGYEGQ
                     GPEHSSARLERFMQLSADTNMQVVQPTTASQIFHVLRRQMIRGLRKPLVIMTPKSLLR
                     NRDATSPLSEFTRGTFQTIIPENKDLKAEKVKRIIVCSGKVYYDLAKRREERGADDTV
                     ILRVEQLYPFPHKVFAAELRKYPAVTDIVWCQDEPQNQGAWFFVQHYIHENMAEGQKL
                     GYSGRAASASPAVGYSHLHQEQQKALVDGAFTKLKGFVLSK"
     gene            complement(7604..9496)
                     /locus_tag="VGA59_11440"
     CDS             complement(7604..9496)
                     /locus_tag="VGA59_11440"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HEX9720336.1"
                     /translation="MSSYAEFHRRSLQDRDAFWAEQARLVDWRTPPQTICDYSRPPLV
                     NWFAGGTTNLCYNAVDRHLKDRADQAALIYVSSETGAERTYSFRQLHEEVQRAAAVLK
                     SLGVQKGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASVSLASRIEDASPRVI
                     ISADAGSRAGKVVAYKPLLDEAIRLSSHKPQSVVLVDRGLAPMERVPGRDHDWSSLGA
                     KQGDAKVECEWLQSTDVSYILYTSGTTGQPKGVQRDVGGYAVALAASMKLIFLGEPGE
                     TYFSTSDIGWVVGHSYIVYGPLIAGMATIMYEGLPIRPDAGIWWSLVEKYKVTSMFSA
                     PTAVRVLKKQDPAFLKKYDLSSLKALFLAGEPLDQPTAQWIADGLGRPIIDNFWQTES
                     GWPILALANGVERQTSKFGSPGLPMYGYDVRLVHESTGEELTRPNEKGVVAIAGPTPP
                     GFMQTVWGDDDRYVKTYWETIPGKMLYSTFDWGIRDEDGYFFILGRTDDVINVAGHRL
                     GTREIEESISSHPNVAEVAVVGVADQLKGQVAVAFVVARDASALHDAAARHTLEGAIM
                     KVVDDQLGAVARPARVYFVSVLPKTRSGKLLRRAIQAVCEGRDAGDLTTIEDPAALQQ
                     IRDLVA"
     gene            complement(9592..10197)
                     /locus_tag="VGA59_11445"
     CDS             complement(9592..10197)
                     /locus_tag="VGA59_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX9720337.1"
                     /translation="MLLSADESQLVLVDYQERLMPFIHEREAVLLNAMRLGRVAQALQ
                     VPTWGTEENPEGLGPNPAQLKALCRKTLSKMRFSACNDGLTELLRAPARQPQGGNARS
                     LPKHLQKAPTPEPERNSIVLAGCETHVCLMQTALDLLEEEFEVWVVTDASSSRSERNR
                     DAAFDRLAGAGAELLTTEMVAFEWLRTAEHPQFKEILSLIK"
     gene            complement(10215..10826)
                     /locus_tag="VGA59_11450"
     CDS             complement(10215..10826)
                     /locus_tag="VGA59_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9720338.1"
                     /translation="MEARLLRQQTLSETRRALVLDAARAVFEEHGIEGANIREIAKRA
                     GYTPGAIYSYFDNKEAIYGALLAESLDRLNAAVQAAMPADATAAAFLNAKAKGWFGFY
                     AANPRELDLGFYLVHGLAPRGLTSELNQRLNARLYEALAPCEQALLAMGLDAPAAARE
                     NTALFAHGVGLLLLQHTGRIRMFRQDATGLFDHYLQALLLRAP"
     gene            10923..12518
                     /locus_tag="VGA59_11455"
     CDS             10923..12518
                     /locus_tag="VGA59_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9720339.1"
                     /translation="MNAPSPPAAMMSGAAYRESLRRLHPTVFVDGRRVDSVADEPALR
                     PGVNAVALTYDYALKPEYEPLMTAVQHTSGKRVNRLSHIDTSAADLLNKLEAVRLVCQ
                     QTGCAQRYLTHDALNAIGQVTARIDDARGVKEHTARFLEFLHRVQDQDLTLAVAMTDA
                     KGDRSLRPHQQANVDSYVHIVERNARGIVISGTKAIVTGAPYVHELLVMPCRNMGQED
                     ADFAVCCSVPIDVAGLTIVARPAGRPGEKVEHGEPLFSRKYGQSTAVCMFDRVFVPWE
                     SVFYAGDWEHSGHLTYSYATHHRQTCIAARAGFGDLLIGAGALMCEANGFDPGRQTHL
                     REQMVELIKIVEGFYACGVAASVYGKADEHSGTFMPDPVFSNIGKLLLSTQIYDMHRI
                     AHYVSGGLIVSLPGPDEDHNPETAAKLSDVLRANPNVPYEQRIQTARFIEDLTAGYQG
                     GWYSMISLHGGGSPAAMKQEIWRNYPVGSKVELVERLLERDVAHDPQRPITSNRQPAR
                     CCDMGCSAPGQPVMVDLPVSRKR"
BASE COUNT         2029 a         4216 c         4124 g         2375 t
ORIGIN      
        1 cccaatgccg ggcctgcact atgcacgtgc cgaggcatga cgctgcggcc cggccccgca
       61 atccatgccg gtggagggcg ctgacgccgc atggatcccg ggtcaagccc gggatgacag
      121 cagcatgtgg gacaggtttc atctcagttg ctccaccgcg catcgcaccc cccgcagcgc
      181 ctgaaagcgc gtggccagtg cggggccacc gcggaaccgg ctttgccggg ccgctggtgg
      241 gccttgcagg ccgcatggcc gcgagacgcg gccattcctc gacctgtcca aaccgacgca
      301 ggtcggtttg gagccgcagg tctcggcccc ctcgaggggg agagggcgaa gcccgagggg
      361 gcatcgccga aggcgcttcg ggggtgggtt ttcacgttag gcgggtgtcc acagtccggt
      421 gttcgagtgc caggaacccg gccgactgca tttcgtgcag gcgcgacacg gtccttggaa
      481 actcatgggc cagcggcccc tcaatgtaca acgcctcggg cggtacctcg gccgacagga
      541 tcaactttac gcggcggtcg tacagcacgt ccaccagcca ggtgaaacgg cgcgcctcgg
      601 aggacatccg catcggcatt tgccgcacgc cgctgagcag caccgtgtgg aactgggcgg
      661 cgatctcgag gtaatcgttc tgcgagcgcg gcccgccgca caaggtcttg aagtcgaacc
      721 agaccacgcc gccggccttg cgccgggcgc gaatttcgcg cgcctcgata tgcagcaccg
      781 gatcctcctc atgcgactcg gccaaccgtg tgaacgcccg gttcatctgt tcgtccgccg
      841 ccgggtccag cggcacgtgg tacagctcca cctgctccag ggcgcggcgg cggtagtcga
      901 ggcctgcgtc caccccgatg acttcgagct tcctgtcgag caggtcgatg gcaggcagga
      961 tccgctcccg gtgaagtccg tccgggtaga gatcatgcgg cttgaagttc gacgtggtga
     1021 cgaagccgac gccgttgtcg aacaatgcat tcagcagccg gtgcaggatc atcgcatcgg
     1081 tgatgtcggc cacgtggaac tcgtcgaagc agatcagccg gtggcgtttc gcgatgcgct
     1141 cgccgagttc gtccaacggg ttcacagtgc cctgcagatc gcccatttcc cgatggacct
     1201 cgcgcatgaa ttcgtggaag tgcagccgcg tcttgcgccg gatgggcacg gccgtgtaga
     1261 agcagtccat caggaagctc ttgccccggc ccacaccccc gtacatgtag accccgcggg
     1321 ggatgtcggg acgtttgatc agcttcttcc agacgttgga gcgctgttcc ttgaattggg
     1381 cccattcgcg cgcgcaccgg tccagcgctt cgacggcgcg caactgcgcg gggtcgctgc
     1441 ggtagccgcg cgcggtgagt tcggcttcgt agacttcttt gaccgacata aaaggtcgtc
     1501 attgcgggcc ccggatcaag tccggggcag gcctgatccg cgatccatgg ctgctctgca
     1561 tgcgggcatg aatcccggga gctgaaaccc ccgagtcgat ccggggccgg gatgacaggc
     1621 cactagaaat tcagcgttcg cttgtccacc gccagggctg cttccttggt ggcctccgac
     1681 agcgaggggt gggcgtggca gatgcgcgct atgtcctcgc tggaggcccg gaattccatc
     1741 gccaccaccg cttcggaaat cagctcgctg gccatcgggc ccacgatgtg cacgccgagg
     1801 atctcgtcgc tggccgcgtc ggccaggaac ttcaccatgc cggtggtatc gcccagcgcg
     1861 cgcgcccggc cgttggccag gaacgggaac gtgccggccc ggtacttcac gcccgccgcc
     1921 ttgagctgct gctcggtctg gccgacccac gcgatttcgg gactggtgta gatcacccag
     1981 ggaatggtgt tgaagttgac gtgcccgtgc tggccggcaa tgcgttcggc cacggccaca
     2041 ccctcttcct ccgccttgtg cgccagcatc gggccgcgca cgacgtcgcc gatggcccag
     2101 acattcggaa ggttggtgcg gcagtcgtca tccacgatca cggcgccgcg ctcatcgagc
     2161 ttcaagccca cggcatccga attcagcccg gtggtgttgg gtgcacggcc gatggaaacg
     2221 atcagcttgt cgacctccag cgtcctcgcc tcgcccttgg cgttggtcca ggcgaccgac
     2281 acgcccttct tgccgtttct gacctcgccg accttcacgc ccagctcgat cttcaggccc
     2341 tgcttgtcga atgctttcct ggcttccctg gcgatctgct cgtccaccag gcccaggaag
     2401 gcaggcagtg cctcgagcac ggtcacttcc gagcccaggc ggcgccagac cgatcccatc
     2461 tccagtccta tgacgccggc accgatcagt cccatcttct tcggcacgcc ctgcacgcgc
     2521 agggcgccgt cgttcgacag gatgttttcc tcgtcgaagg gcgcacccgg gagcgcgcgg
     2581 gcgttggagc ccgtggccac cacgatgtgc tttccgacga tcgtttcctc ggccgccgcg
     2641 gtcatcttga tctcatagcc gccctcgccc gctttcacga acgatccgcg gccatggaag
     2701 aacgaaacct tgttcttctt gaacaggaag gcgatgccgt cgttgttctg cttgaccacc
     2761 tggtccttgc gcgccagcat cttcgccaga tccagcccca ggcccttcac ctcgatgccg
     2821 tggtcggcga agtgcttgcc ggcatgctcg tagtgttccg aggactgcag cagcgccttg
     2881 gacggaatgc agccaatgtt ggtgcaggtc ccgcccaggg cgggcccgcc cttgctgttc
     2941 ttccactcgt cgatgcaggc cgtgttgaaa cccagttgcg ccgcgcggat ggccgcgatg
     3001 tagccgccgg ggcccccgcc gatgacgatg acgtcgaatt gcttattcat tgtgtttttc
     3061 cttgcttgtc atgccgggct cgacccggca tccatgcatc gccgaccgaa acgcgatccg
     3121 aagctgcatg gattgcggat caagcctgcc ccgggcttga tccgggggtc cgcaatgaca
     3181 gtcatcctca aatatcgaac agcagccgcg ccggctcttc cagcgcttcc ttcatcgcca
     3241 ccagccccag cacggcctcg cggccgtcga tgatgcggtg gtcgtagctc atggccaggt
     3301 agttcatcgg ccgcaccacg acctgcccgt tctccaccac cgcccggtcc ttggtcgcgt
     3361 gcacgcccag gatcgccgac tgcggcggat tgatgatggg ggtggacagc atcgagccga
     3421 acacgccgcc gttggagatg gagaaggtgc ctccggtcat ctcctccagc cccaacttgc
     3481 cgtcgcgtgc cttgacaccg tactcggcga tctttttctc gatgtccgca aaactcatct
     3541 ggtcggcgtt gcgcaggatc ggcacgacca ggccgcgcgg cgaaccgacg gcgattccga
     3601 tgtcgaagta gccgtggtag acgatgtcgt tgccgtcgac cgaggcattg atcacggggt
     3661 actttttcag cgcatgcacg gccgccttga cgaaaaagct catgaagccg atcttgacgc
     3721 cgtgctcctt ctcgaacttt tcctggaact tcttgcgcat ttccatcacg ggcgccatgt
     3781 tcacttcatt gaaagtcgtg aggatcgcgc tggaggactg cgactggagc agccgttcgg
     3841 ccacccgtgc gcgcagtcgg ctcatgggca cgcgctgttc gggccgctcg ccgaggtccg
     3901 gcgcggccac cggcgcctgg acctggggca gcaccggctt cgccgcggcg gccggcgagg
     3961 tggccggcgc ggcaaccgcc ttgccggcga ccgcccccag cacgtcgccc ttggtcaccc
     4021 ggccgtccct gccggtgccg ggcaccgaac ccgcggccag cttgttgtcg gccatgatct
     4081 tggcggcggc cggcatcgcg atgccggcct tcgagccagg ggcggcgagc ggcgccgcgg
     4141 ctggcggcac ggcggcgggc gctgcggccg tgggcgccgc ttccgcggca ggcgcacccg
     4201 cggcggcctt accttcggtg tcgatcttcg cgatcacctg ctccgccgtc accgtgctgc
     4261 cgtccggcac cacgatctgc gccagcacgc ccgcggcggg tgccggcacc tccagcacca
     4321 ccttgtcggt ttcgatttcg atgacgattt catcgagcgc gacgagctcg ccggcctgtt
     4381 tcttccactg cagcagggtc gcctcggcaa ccgattcgga cagctggggg accttcactt
     4441 cgacgatggc cattttttgt tcctgaagaa tcttgtatgc gcgtagccgg accccacccg
     4501 cgcagcgggt cgggtccggc ttctcgcctt atttactgag aacgaagccc ttgagcttgg
     4561 tgaaggcgcc gtccaccagg gctttttgct gttcctggtg caggtgcgag tagccgaccg
     4621 cgggagaggc cgaggcggcc cggccggaat agcccagttt ctgcccctcg gccatgttct
     4681 cgtggatgta gtgctgcaca aagaaccagg cgccctggtt ctgcggctcg tcctggcacc
     4741 agacgatgtc ggtgaccgcg gggtatttcc tcagctcggc ggcgaacacc ttgtgcggga
     4801 acgggtagag ctgctcgacc cgcaggatga ccgtgtcgtc cgcgccccgt tcctcgcgcc
     4861 gctttgccag gtcgtagtag accttgcccg aacagacgat gatgcgcttg accttctcgg
     4921 ccttgaggtc cttgttctcg gggatgatcg tctggaaagt tccccgggtg aactccgaca
     4981 agggtgaggt ggcgtccctg ttgcgcagca gcgacttggg cgtcatgatc accaggggct
     5041 tgcgcagccc acggatcatc tggcggcgca ggacatggaa gatctggctg gcggtggtcg
     5101 gctgcaccac ctgcatgttg gtgtcggccg acagctgcat gaagcgctcc aggcgcgccg
     5161 agctgtgctc gggaccctgg ccttcatagc catgcggcaa catgagcgtg atgccgttga
     5221 tccgccccca cttgacttcg cccgaggcga tgaactggtc gatcaccacc tgggcgccgt
     5281 tgacgaagtc gccgaactgc gcctcccaga tcaccagcgt gttcgggtcg ttggaggcgt
     5341 agccatattc gaagccgagt accgcttcct ccgacaggat ggaatcgatg atgacgaaag
     5401 gcgcctggtt ctctgcgacg ttttccagcg gcgtgtaggt gccggtgtcc cacttctccc
     5461 gcttctggtc gtgcagcacg gcatggcggt ggctgaacgt gcctcgggcg ctgtcttcgc
     5521 ccgagagccg caccgggtag ccgctggcga ccagcgaggc gaatgccatg tgttcgccca
     5581 tgccccagtc cacgccggtt tcgccgcggc ccatggcggc acggtcgtcc agcacctttt
     5641 tcaccagcgg atgggccgtg aaattcgccg gtattgtggt gatcttcccg gccagccgct
     5701 tccactcggc catcggaatc gccgtgtccg cgctgtcggt ccacttcttg cggaggaaag
     5761 gcgtccagtc caccgcgtac ttgctcttga agttggtcag caccgggtcg gtcgtgtgct
     5821 tgcccgcatc catcgccgcg cggtagccct tgaccatgtc gtcgcccagc gtttcgccca
     5881 gcccctgcgc agcgagcttg tccgcataga gcttgcgggt gcccgggtgg gcggcaatct
     5941 tcttgtacat gagcggctgg gtcagcgagg gcgtgtcctg ctcgttgtgg ccgagcttgc
     6001 ggaaacacac gatgtccacc acgacgtcct tggcgaactc catgcggtat tccagcccga
     6061 gctgggtcgc cagcacgacg gcttccgggt cgtcgccgtt cacgtgcagc accggcgcct
     6121 cgatcatctt gaccacgtcg gtgcaataca gcgtcgagcg gctgtcgcgc ggatcgctgg
     6181 tggtgaagcc gatctggttg ttgatcacga tgtgcacggt gccgccggtg aagtagccgc
     6241 gcgtttcggc cagcgccagc gtttccatga ccacgccctg ccccgcgaat gcggcgtcgc
     6301 cgtgtacctg gaccggcagc acctgtttgc cttgcgggtc ggcacgccgg tccatgcggg
     6361 cccgcaccga accttcgacg acggggttga cgatttccag gtgggagggg ttgaaggcca
     6421 ggctcaggtg gaccgggccg ccgcgcgtgg tcacgtcgga gctgaagccc tggtggtact
     6481 tgacgtcgcc ggacggcaat tcctccttgg ccgtgtggtc gaactcggcg aacaggtcgg
     6541 ccggcatctt gcccagggaa ttgaccagca cgttcaggcg gccgcggtgc gccatgccga
     6601 tcacgatctc ctgcactccc ttggcgccgg cctcctggat cagctcgtcc atggaagcga
     6661 tgaaactttc gccgccttcc agtgaaaagc gcttctggcc gacatacttg gtgtgcagga
     6721 aacgctccag gccttcggcc gccgtgacgc gctcgaggat gtgcttcttc ttctcgatgg
     6781 aaaagttcgg cttgctgcgg atcgattcga gctgttgctg ccaccagcgc ttcttgtact
     6841 ggtcgctgat gtacatgtac tcggcgccga tggcgccgca ataggtctcg cgcagcgcgt
     6901 tgatgagctc gcgcagcgac atgctttcct tgccgaagaa ggtgttgctg atgttgaaca
     6961 ccagctcctg gtcggcatcg ctgaatccat agaaggccgg gtccagctcg gggatctttt
     7021 cgcgctcggc gcgcttgagc gggtcgaggt cggcccagcg gctgccgacg ttgcggtagg
     7081 cggcgatgag ctgctgcacc gcggtgcgct tgcgccccat cgcggcttcg ccgctgcccg
     7141 gcaccagctt gatgccgccc tgcttggcac gctcggcgaa cgcattgatg accggcatgt
     7201 gcggcacatc cttcgcgttg ctgccgtcgg cagccggcac gttctggagc gcgtcgaagt
     7261 agtcgcgcca ggtttcggga acgctggtgg gattggcgag gtagttttcg tacatctcct
     7321 cgacataggg cgcattgccg ccgaagagat aggaattgcc ctggtaggcc gtgtagacgg
     7381 agctggtctc actcatgcgc tgactttccg cctccctgca ggagacattg gctggttgtg
     7441 gaaaaccttc cgcgacacgg ctgaaccgga tggcggatgc gactgtggct ggaaggggcc
     7501 gttgttgcga cccgcattgt gccactattt gccgcaggca tcgcctgcag ggaatacgtt
     7561 acagccggcc gcgtccgtcg cggccggctg tttccggctt gcttcaggcc accagatctc
     7621 ttatctgctg cagcgcggcc gggtcctcga tcgtggtgag atcgccggcg tcgcggcctt
     7681 cgcagaccgc ctggatcgcc cggcgcagca gcttgccgct gcgggtcttg ggcaggacgc
     7741 tgacgaaata cacccgcgcc ggcctggcga cggcgccaag ctggtcgtcg accaccttca
     7801 tgatggcgcc ttcgagcgtg tgcctggcag cggcgtcgtg cagcgccgac gcgtcacgcg
     7861 ccaccacgaa ggcgacggcc acctggccct tgagctggtc ggcgacaccc acgaccgcca
     7921 cttcggcgac gttcggatgg ctcgagatgc tctcttcgat ctcgcgggtg cccagacggt
     7981 ggccggcgac gttgatcacg tcgtcggtac ggccgaggat gaagaagtag ccgtcctcgt
     8041 cgcgaatgcc ccagtcgaac gtgctgtaga gcatcttgcc ggggatcgtt tcccaatagg
     8101 tcttgacgta gcggtcgtca tcgccccaga cggtctgcat gaagccgggc ggcgtcggcc
     8161 cggcgatcgc gaccacgccc ttttcgttgg gccgggtcag ttcctcgccc gtgctctcgt
     8221 gcaccagcct gacgtcgtag ccgtacatcg gcagtccagg actgccgaac ttgctggtct
     8281 ggcgctcgac accgttcgcc agggccagga tgggccagcc gctttccgtt tgccagaagt
     8341 tgtcgatgat cgggcggccc aggccgtcgg cgatccattg ggcggtgggc tggtccagcg
     8401 gctcgcccgc caggaacagc gccttcaggc tcgacaggtc gtacttcttg aggaacgcgg
     8461 gatcctgctt cttgagcacc cgcactgcgg tgggggcgct gaacatggac gtcaccttgt
     8521 atttctcgac caggctccac catatgccgg cgtcgggccg gatcggcagg ccctcgtaca
     8581 tgatcgtggc catgcccgcg atcagggggc cgtacacgat gtagctgtgc cccacgaccc
     8641 agccgatgtc gctggtggag aaataggttt cacccggctc gccgagaaag atgagcttca
     8701 tgctcgccgc cagcgccacg gcgtagccgc ccacatcgcg ctgcacgccc ttgggctggc
     8761 cggtggtgcc gctggtgtag aggatgtagc tgacgtcggt ggactgcagc cattcgcact
     8821 cgaccttcgc gtcgccctgc ttggccccca ggctgctcca atcgtgatcg cgcccgggga
     8881 cgcgctccat cggcgccagg ccgcggtcca ccagcaccac ggactgcggc ttgtggctgg
     8941 acagccgtat cgcctcgtcc agcagcggct tgtaggccac caccttgccg gcgcgcgagc
     9001 ccgcgtcggc actgatgatg acgcggggcg acgcatcctc gatgcgcgaa gcgagcgaca
     9061 ccgaggcgaa gccgccgaat accaccgaat ggatcgcgcc gatgcgcgcg caggccagca
     9121 tcgcgaacgc cgcctcggcg atcatcggca tgtagatcag cacccggtcg cctttttgca
     9181 cgccgaggct cttgagcacg gcggctgcgc gctggacctc ttcatgcagc tggcggaagc
     9241 tgtacgtgcg ctcggcgccc gtttcactgg acacgtaaat cagggccgcc tggtcggcgc
     9301 ggtccttcag atgccggtcc acggcgttgt agcagaggtt ggtggtgccg ccggcaaacc
     9361 agttgaccag cggggggcgg ctgtagtcgc agatcgtttg cggcggcgtc cgccagtcga
     9421 ccaggcgggc ctgttcagcc cagaaagcgt cgcgatcctg tagcgaccgg cggtgaaatt
     9481 ctgcgtagct gctcatttat gtctccaaat ggtgaaccgg cgctgcacat tgtggacttt
     9541 tggcttgccc caagctgacg gcatggctga ttggacgcct cgtcggcgcc atcatttgat
     9601 caagctcaag atttccttga actgcggatg ttccgccgtg cgcagccact cgaaggcgac
     9661 catttcggtg gtcaagagtt cagcgccggc gccggccagg cggtcgaagg cggcatcccg
     9721 attgcgttcg ctgcgcgagc tggaggcgtc cgtgaccacc cagacctcga attcctcctc
     9781 cagcaggtcc agggcggtct gcatcaggca gacatgggtt tcgcagccgg ccaggacaat
     9841 gctgttgcgc tccggctccg gcgtcggcgc cttctgcagg tgcttgggca ggctgcgggc
     9901 atttccccct tggggctggc gagcgggtgc gcgcagcagt tccgtcaggc cgtcattgca
     9961 ggcgctgaag cgcatcttgg acagggtctt gcggcacaag gctttcagct gcgccggatt
    10021 tgggcccagc ccctcgggat tttcttccgt gccccaggtc ggcacctgca gggcctgcgc
    10081 cacgcggccg agccgcattg cattgagcag caccgcctcg cgctcgtgga tgaatggcat
    10141 caggcgctcc tggtagtcca ccagcaccag ctgggactcg tcggcggata gcagcatctg
    10201 gctcgtctcc tcggtcaggg cgcccgcagc aacagcgctt gaaggtaatg gtcgaacagg
    10261 ccggtggcgt cctgcctgaa catccggatg cggccggtat gttgcagcag gagaagcccc
    10321 acgccatggg cgaacagcgc cgtattttcc cgcgccgcgg ccggggcatc cagccccatg
    10381 gccagcaagg cctgttcgca gggagccagc gcctcgtaca gcctcgcgtt gaggcgctgg
    10441 ttcaactccg atgtcaggcc acggggcgcc aggccgtgca ccaggtagaa gcccaggtcg
    10501 agctcgcgcg ggttggcggc atagaagccg aaccagccct tggccttggc gttgaggaaa
    10561 gccgccgcgg tggcgtccgc cggcatggct gcctgcaccg cggcgttcag ccggtccagc
    10621 gattcggcga gcaaggcccc gtaaatggct tccttgttgt cgaaatagga atagatcgcc
    10681 cccggcgtgt agcccgcgcg tttcgcgatc tcgcggatat tagctccttc gattccatgt
    10741 tcctcgaaca ccgcgcgagc tgcgtccagc accagggcgc ggcgggtttc gctcagggtc
    10801 tgctggcgca gcagtcttgc ttccatgcgc ggactataaa gccaaaccgc gggtcgaatg
    10861 aacgttgaca gcatttttga acgctgttca aatttggcat ccctcgagcg caggagccga
    10921 ccatgaatgc cccatctccc cccgccgcga tgatgagcgg cgcagcctac cgcgagtcgc
    10981 tgcgacggct gcaccccact gttttcgtcg acggccggcg agtggatagc gtggccgacg
    11041 aaccggcctt gcgccccggc gtcaacgcgg tcgcgctgac ttacgactac gcgctgaagc
    11101 cggagtacga gccgctgatg acggcggtgc agcacaccag cggcaaacgg gtcaaccggc
    11161 tcagccacat cgacaccagc gcggcggatc tgctgaacaa gctggaggcg gtgcggctgg
    11221 tctgccagca gacaggctgc gcacagcgct acctcaccca cgatgcgctc aacgccatcg
    11281 gccaggtcac ggcacggatc gacgacgccc gcggcgtgaa agagcacacg gcacgcttcc
    11341 tggaattcct gcaccgggtg caggaccagg atctcaccct ggcggtggcg atgaccgacg
    11401 ccaaaggcga ccgcagcctg cgcccgcacc aacaggccaa tgtggacagc tacgtgcaca
    11461 tcgtggagcg caacgccagg ggcatcgtga tctcgggcac caaggctatc gtgaccggcg
    11521 cgccctacgt tcacgaactg ctggtgatgc cctgccgcaa catgggccag gaggatgccg
    11581 acttcgccgt ctgctgctcg gtgccgatcg acgtggccgg cctgaccatc gttgccaggc
    11641 ccgccggccg ccccggcgaa aaggtcgagc acggcgagcc cttgttctct cgcaagtacg
    11701 gccaaagcac ggccgtctgc atgttcgacc gcgtcttcgt cccgtgggaa agcgtgtttt
    11761 atgccggtga ctgggagcac agcgggcatc tcacctacag ctatgccacg caccaccggc
    11821 agacctgcat cgcggcgcgc gccggattcg gcgacctgct gatcggcgcg ggcgcgctga
    11881 tgtgcgaggc caacggcttc gatcccggca ggcaaaccca cctgcgcgag cagatggtgg
    11941 agctgatcaa gatcgtggaa ggcttttacg cctgcggcgt ggcggccagc gtctacggca
    12001 aggccgacga gcacagcggc acgttcatgc ccgacccggt gttctccaat atcggcaagc
    12061 tgctgctgtc cacacagatc tacgacatgc accggatcgc ccactacgtg agcggcggcc
    12121 tcatcgtcag cctgcccggc cccgacgagg accacaaccc cgagacggcg gcaaaactct
    12181 ccgacgtgct gcgcgcgaat cccaacgttc cttacgaaca gcgcatccag accgcgcgtt
    12241 tcatcgagga cctgacggcc ggctatcagg gcggctggta cagcatgatc agcctgcacg
    12301 gcggcggctc gccggcagcc atgaagcagg agatctggcg caactatccg gtcggctcca
    12361 aggtggagct ggtcgaacgc ctgctggagc gggacgtggc gcacgatccg caacgcccca
    12421 tcacgagcaa ccgccagccc gccaggtgct gcgacatggg ctgcagcgcc ccggggcagc
    12481 cggtgatggt ggacctgccg gtgtcgcgaa agcgctgaaa cgacgtttga gatcccaacg
    12541 aaaattgtca tgccggactt gatccggcat ccttgacttc ctggattgcg ggtcaagccc
    12601 gcaatgacat cgaaaggacg gtctcagcag cacctacagc gacagcaggc gctggaagaa
    12661 ggagcggtag cggcgcagag ccacccgcag gttctccgtg gaggcgctgt cctgttgctc
    12721 gagctggctt tctaacctgt cgcg
//
LOCUS       DASWIY010000314         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1310515, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000314 DASWIY010000000
VERSION     DASWIY010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310515"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VGA59_11460"
     CDS             complement(<1..294)
                     /locus_tag="VGA59_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9720340.1"
                     /translation="MSQAERLAKLRYLLDEGRWPTRPFLLDTLRVSPATLKRDRMNEP
                     VVFDRERRGWRLDPAAQLAGTQYELPGLWLSAEEIHALLTMQHVLAHLDTGGLL"
     gene            434..892
                     /locus_tag="VGA59_11465"
     CDS             434..892
                     /locus_tag="VGA59_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX9720341.1"
                     /translation="MKLLRLVLFAAATLGFVNLASAQTAGTREEAKAMVDAAVEHVKA
                     VGPEQAFKDFTEDKAKWTNKDLYVMAYDNNGKCVGHGANPKLIGKDLLELKDPNGKPL
                     IRELIDTAKKGGGWVDYDWPQPQTKRLEGKSTYARKLANFDGWVGVGVYR"
     gene            933..>1614
                     /locus_tag="VGA59_11470"
     CDS             933..>1614
                     /locus_tag="VGA59_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720342.1"
                     /translation="MLQWLKNAPVGVKVALAPALGVLCLAFMGLLGGYANVKLGGALS
                     KLSGERLPRVVMVANAQRRLVRLNALVNQSLAWEAAGFKADKIEALDKRIVSGLDSFS
                     GQLKEFAADPALDATEREATQQLVQDYAKFRKSAVDALDIKSGMVANAATFMTMMEAN
                     FNDLTSMFEKLVAHEQALADEAAAEGKNLANRNNLLILGGGVLATALAAGFAWLSSRL
                     IVIPLRQAS"
BASE COUNT          316 a          489 c          531 g          278 t
ORIGIN      
        1 gagcaggccg ccggtgtcca ggtgagccag cacgtgctgc atggtcagca gggcgtggat
       61 ttcttcggcg ctcagccaca ggccgggcag ttcgtattgg gtgcccgcga gctgggcggc
      121 ggggtcgagg cgccagcccc ggcgctcgcg gtcgaagacg accggctcgt tcatgcggtc
      181 tcgcttcagg gtggcggggg agacgcgcag ggtgtcgagc agaaagggcc gggtcggcca
      241 gcgcccttcg tcaagcaagt acctgagctt tgccagccgc tcggcctgtg acatgccatg
      301 tatccggttc gacgggcgct tgaccgaagg tgaatgtcaa caatggtcaa acgaattcca
      361 gcgcatgccc atccagtcga tattgaaggg gcgggtgctg atgcaccgca cccatcaacc
      421 cctggagatc accatgaaac ttcttcgcct tgttctcttt gccgccgcca ctcttggctt
      481 tgtcaacctc gcttcggcac aaaccgccgg aacgcgcgag gaggccaagg cgatggtcga
      541 tgcggccgtc gagcatgtga aggccgtggg ccccgagcaa gcgttcaagg acttcaccga
      601 agacaaggcg aagtggacga acaaagacct gtacgtcatg gcgtatgaca acaacggcaa
      661 gtgcgttggc catggcgcca accccaagct gattggcaag gatctgctcg aactcaagga
      721 tccgaacggc aagccgctga tccgcgaact catcgacacc gccaagaagg gcggcggctg
      781 ggtcgactac gactggcccc agccgcagac caagaggctc gaaggcaagt ccacctacgc
      841 gcgcaagctg gccaacttcg acggctgggt cggagtgggc gtgtaccgct gactcgtctg
      901 acgtcacgcc gtcgagacca caggagagtc gtatgttgca gtggctgaaa aacgcccccg
      961 tgggggtcaa ggtcgctctc gcacccgccc tcggcgtgtt gtgcctggca ttcatggggc
     1021 tgctgggagg ctacgcgaac gtgaagctcg gaggcgccct gtccaagctc agcggcgagc
     1081 gccttccccg cgtcgtgatg gtcgccaacg cgcagcggcg gctcgttcgc ctcaacgccc
     1141 tggtcaatca aagcctggcg tgggaggcgg ctggtttcaa ggcagacaag atcgaagcgc
     1201 tggacaagcg gatcgtcagt ggcctcgatt cgttctcagg ccagctcaag gagtttgccg
     1261 ccgatccggc gctggacgcg accgagcgcg aagccacgca gcaactggtg caggactacg
     1321 ccaagttccg caagtcggcc gttgatgcgc tggacatcaa gtcgggaatg gttgcgaacg
     1381 cagccacgtt catgacgatg atggaggcga acttcaacga cctgacgtcg atgttcgaaa
     1441 aactcgtcgc gcatgaacag gcgctggctg atgaggccgc agcggaaggc aaaaacctcg
     1501 ccaaccgcaa caaccttctc atcctggggg gtggcgtgct ggccacggcg ctggctgccg
     1561 ggtttgcgtg gctgtcttcc aggctgatcg tcattccgct tcggcaggct tcca
//
LOCUS       DASWIY010000315         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2800195, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000315 DASWIY010000000
VERSION     DASWIY010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2800195"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VGA59_11475"
     CDS             complement(<1..186)
                     /locus_tag="VGA59_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004555917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720343.1"
                     /translation="MKLKLVRLGTAALAAVAMALAGCGGGGGGGGGGDTDAGPVQPVQ
                     QTLAAAAAQSSNDTSTNP"
     gene            complement(640..1839)
                     /gene="nrfD"
                     /locus_tag="VGA59_11480"
     CDS             complement(640..1839)
                     /gene="nrfD"
                     /locus_tag="VGA59_11480"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX9720344.1"
                     /translation="MEHLIPFAPYTGWVYPNETVFEWSVLIAVYPYLTGLVAGAFTVS
                     SLYQVFGFERLKPVAHLALLTSLCFMIFVPLPLLMHLGHPERAFNSMTTPHLTSAMAM
                     FGFFASFYVGLLILEGWFAYRPFIVEQAQKRTGLLGRFYRLLTLGSHDLSENAMRYDK
                     KWIFALAVIGIPAAHGLHGYVGFMYGSLKSREWWSSDLMPVIFLFSAIISGVSLLAVL
                     YVASCKLRKIPVDLACLKGLAYTIWAFIMVALVLEGLEFANIVYKGREGVDMIMQYVT
                     GPLLVPYFILQFAVGAITPFLWLSYMIWRGTTGRALVVGVTVCALLVLFSVFMMRWNV
                     VIGGQEISKTGKGLLSYHMPLMGKEGALVAIALTLAPLALLFGLTRLFPPWEDKAHLT
                     EAQAVGH"
     gene            complement(1839..2735)
                     /locus_tag="VGA59_11485"
     CDS             complement(1839..2735)
                     /locus_tag="VGA59_11485"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9720345.1"
                     /translation="MSEKAELSMQARRAFLGKLAATCTGAAVGAGVLGAVQAQDAKSG
                     NSAGGAPDGAPAGNVKAASTAAGGSFPTPDYDWTKHQWAFGVDATQCIGCLRCVEACK
                     RENDVPGNAHQFRTWVERYVYLEGEEHVRIDSQQDPVNIEASGSQKEFRFANRYKDAK
                     VDKAFFVPKLCNQCTHPACVQVCPVGATYRTKDGVVLIDHDYCIGCRYCVQACPYGAR
                     YFDEEKGVSDKCTWCYHRITKGLEPACVEVCPVGARVFGDTRDKQSPISLFIRNNRVQ
                     VLRPESGNAPNVFYVGIDKEVN"
     gene            complement(2738..>3523)
                     /locus_tag="VGA59_11490"
     CDS             complement(2738..>3523)
                     /locus_tag="VGA59_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9720346.1"
                     /translation="HKSVTCEVCHGAALGHPENGKLPIPKDTAKLCTLCHEAMPGRPS
                     TQPQIVLAQHNPLATSGQQCAACHNPHSPKIAAGLVKVTGNAAMGKKRADEDCAACHG
                     DDGNSPNNTWPTLAGQNAAYLARILTAYKSGDQEDVAMTPLAKELADADIQNLAVYYA
                     GSSCKSPAQVKSVGDAAIGKTLAKNCAGCHGETGIAGNPAWPHVAAQKPGYLINALKA
                     FRSGQRKDPMMAGVSRGLSDEDIANLAAYYAVQSCPPATQGRK"
BASE COUNT          649 a         1087 c         1121 g          666 t
ORIGIN      
        1 ggggttggtc gaagtgtcgt tcgacgattg cgcggcggcc gcggccagcg tctgctgcac
       61 cggttgcacg gggcccgcgt ccgtgtcgcc gcctccgcct ccgcctccgc ctccgccgca
      121 gcctgccagg gccatggcga ccgcggccaa agccgctgtt cccaacctta ccaacttgag
      181 cttcatatgt gactccgtga cgtctgacga cgaactttct cgactttcaa atacgggtgg
      241 tcgtcgcaga cgctgaacgc gtcgttgatt ccttccttga cccacatcaa ccgttggcag
      301 cagttattgg tgtattagca gctgcgcagc tcagtggaaa agggcgactt cgacctgtgc
      361 tgagattgtc gaagtcaggg tttgcccgcg ccatcggaag cagctcaaga ttgccgtagg
      421 aaaactccga gagtggttac tgtgcgtgca aagtcagctc ggagaaatcg atgagctgcg
      481 gacgcgtccg gcggcaagaa cgcgtccggg cgggggcgga cgccgcgccg ctttcgggcg
      541 gcatcgcgcc cttgcgcttc acgagttgac ggtggccgaa aaatgatcag gcgtgcactg
      601 caggacggag cgattccatt ccctgcccac cccgttcatt cagtggccga cagcttgcgc
      661 ttcggtcagg tgggctttgt cttcccaggg cggaaacagc cgggtcaatc cgaacaacag
      721 ggcgagcggc gcgagcgtca acgcgatcgc caccagcgcg ccttccttgc ccatcagcgg
      781 catgtgatag gacaggagcc ctttaccggt cttggagatt tcctgtccgc cgatcaccac
      841 gttccagcgc atcatgaata cggagaacag caccagcagc gcgcagacgg tcacccccac
      901 gaccagggcc ctgccggtgg tgccgcgcca gatcatgtag gaaagccaga ggaacggcgt
      961 gatggcgccg acggcgaact gcaggatgaa gtacggaacc agcagcgggc cggtgacgta
     1021 ctgcatgatc atgtccacgc cctcgcgacc cttgtagacg atgttggcga attcgagacc
     1081 ttccagaacc agggcgacca tgatgaaggc ccagatcgtg taagcgaggc ccttgaggca
     1141 tgccaggtcg accggaatct tgcgcagctt gcacgaggcc acatagagca ccgccagcag
     1201 cgaaacccct gagatgatcg ccgagaacag gaagatgacc ggcatcaggt cggaagacca
     1261 ccattcgcgc gacttgagcg aaccgtacat gaagccgacg tagccgtgca ggccgtgggc
     1321 cgccggtatg ccgatcacgg cgagtgcgaa tatccacttc ttgtcgtagc gcatggcatt
     1381 ttcggacaag tcgtgcgagc ccagggtgag caggcgataa aaccgcccga gcagcccggt
     1441 tctcttttgc gcctgctcca cgatgaaggg ccggtacgca aaccagcctt caagaatcag
     1501 gagaccgacg tagaagctgg cgaaaaagcc gaacatcgcc atcgcggacg tcagatgcgg
     1561 cgtggtcatg gagttgaatg cgcgctccgg gtgccccagg tgcatcagca aaggcagcgg
     1621 cacgaaaatc atgaagcaca gcgaagtcag cagcgccagg tgcgccaccg gcttcagacg
     1681 ctcgaaaccg aacacctggt acaggctcga cacggtaaac gcgccggcaa ccagtccggt
     1741 gaggtaggga tagacggcga tcaggaccga ccactcgaat accgtttcgt tcggatagac
     1801 ccacccggtg tagggtgcaa acgggatgag atgttccatc agttcacttc cttgtcaatt
     1861 ccgacataga agacattcgg ggcgttgccg gattccggcc tcagcacctg aacgcggtta
     1921 ttgcgaatga acaggctgat cggactttgc ttgtcccggg tatcgccgaa aacgcgcgcg
     1981 ccgaccgggc acacctccac gcaagcgggc tcgagcccct tggtgatgcg gtgatagcac
     2041 caggtgcatt tgtcggaaac acccttttcc tcgtcgaaat agcgggcgcc gtaggggcag
     2101 gcctgcacgc aatatctgca accgatgcag tagtcgtggt cgatcagcac cacgccgtct
     2161 ttggtcctgt aggtcgcgcc gacggggcat acctgcacgc aggccggatg ggtgcactgg
     2221 ttgcacagct tgggaacgaa gaaggccttg tcgaccttcg catccttgta gcgattggcg
     2281 aatcgaaact ccttctgcga acccgatgcc tcgatgttca ccgggtcctg ctggctgtcg
     2341 atgcgcacgt gttcctcgcc ctcgagatag acgtagcgtt cgacccaggt gcggaattgg
     2401 tgtgcgttgc ccgggacgtc gttttcgcgc ttgcaagcct cgacgcagcg caggcagccg
     2461 atgcattggg tggcgtcgac gccaaacgcc cattggtgct tggtccagtc gtaatcgggc
     2521 gtcggaaagc tgccacccgc ggcggtggag gcggctttca cattgcctgc gggggcgccg
     2581 tcgggggcgc cgcctgccga attcccactc ttggcatcct gggcctggac ggcgcccaac
     2641 acgccggcac ccacggccgc acccgtgcag gtggccgcca gcttgccgag aaacgcgcgc
     2701 cgcgcctgca ttgacaattc ggccttctcg ctcatggcta cttccttcct tgggttgcgg
     2761 gcgggcagct ctgcacggcg tagtaggcgg cgaggttcgc gatgtcctcg tcactcaagc
     2821 cccgggaaac acccgccatc atcgggtcct tgcgctgtcc cgacctgaag gccttgagcg
     2881 cgttgatcag gtagcctggc ttctgtgccg cgacatgtgg ccacgccggg ttgccggcga
     2941 tgccggtctc gccatggcag cctgcgcagt tcttcgccag ggtcttgccg atcgcggcat
     3001 cgccaaccga cttgacttgt gccggcgact tgcagctgga accggcgtag tagaccgcga
     3061 ggttctgaat gtcggcatcc gcgagctctt tggcgagcgg cgtcatcgcg acgtcttcct
     3121 ggtcgccgct cttgtatgcg gtgaggatcc tggcgagata agcggcgttc tgcccggcca
     3181 gggtcggcca cgtattgttg ggactgttcc cgtcatcgcc gtggcaagcc gcgcaatcct
     3241 cgtcggcgcg cttcttcccc atagccgcgt tgcccgtcac tttcaccagg ccggccgcta
     3301 tcttggggga atgcgggttg tggcaggcgg cgcattgctg cccgctcgtt gccagcgggt
     3361 tgtgttgcgc cagcacgatc tggggctggg tggaggggcg tccgggcatt gcctcgtgac
     3421 acaaggtaca caacttcgcc gtgtccttgg ggatcggcag cttgccgttc tcgggatggc
     3481 ccagggctgc gccgtgacac acttcgcagg tgacggattt gtg
//
LOCUS       DASWIY010000316         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1312001, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000316 DASWIY010000000
VERSION     DASWIY010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312001"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VGA59_11495"
     CDS             <1..874
                     /locus_tag="VGA59_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX9720347.1"
                     /translation="LLSMEEGLAFSGKSAKINPRVVFETWKQLPQYWVTSLRSALVGI
                     WMGITPGGATPASFMSYGLAKKMSRDGAKFGTGQIEGVVAPETAAHAAGTSALLPMLA
                     LGIPGSPTAAVLLGGLLIWGLQPGPLLFVEQKDFVWGLIASMYLGNLVGLIVVLTTVP
                     LFASILRIPFSIIAPVIVVICAIGAYTVHNAMLDIWLMMLFGAVGYVFKKLDYPLAPL
                     VLALVLGDKAEDSFRQAMLLSQGELSILWSNPLVGSISTLALVLLLWPVVSLVIARFR
                     PSKSRDFVAEQPVD"
     gene            910..1743
                     /locus_tag="VGA59_11500"
     CDS             910..1743
                     /locus_tag="VGA59_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEX9720348.1"
                     /translation="MPVIAMTQEMGSLAKDVALELALKGKLDVMRHEVLDNVAQKMHV
                     PSSVISRLREGRAGLVERLTTDPQRVAVYTAAEVFALAAKGNIVLRGWGTTCLLRPVQ
                     HVITVRITRPFALRVKWLMEHLELDDESFAATEVRRSDEAHAAKIKAQFGVTWGDPLL
                     YNMVLNTDRMSVETCAQQILHLAGRPEFQETAQSRAMLADLALSAAVRAALKENEPTR
                     ETNIEIESQGGKVMLRGIVLNAQERAEAARVAGTVAGVGDVDNQLRLMVISRRFTYAK
                     T"
BASE COUNT          289 a          569 c          607 g          318 t
ORIGIN      
        1 cctgctgtcg atggaggaag gcctggcgtt ttccggcaag agcgccaaga tcaatcccag
       61 ggtggtgttt gaaacctgga agcagctgcc gcagtactgg gtgacctcgc tgcgcagcgc
      121 gctggtcggc atctggatgg gcatcacgcc cggtggcgcc acgcccgcct ctttcatgag
      181 ctacggcctg gccaagaaaa tgtccaggga cggcgccaag ttcggcaccg gccagatcga
      241 gggcgtggtc gcgcccgaaa cggcggccca cgccgccggc accagcgccc tgctgcccat
      301 gctggcgctg ggcattcccg gctcgcccac ggccgccgtg ctgctgggcg gcctgctgat
      361 ctggggcctg cagcccgggc cgctgctgtt cgtcgaacag aaggacttcg tctggggcct
      421 gatcgcgagc atgtacctgg gcaacctggt gggcctgatc gtggtgctga ccacggtgcc
      481 gctgttcgcc tccatcctgc gcatcccgtt ctcgatcatc gcgcccgtga tcgtggtgat
      541 ctgcgcgatc ggcgcctaca ccgttcacaa tgccatgctg gacatctggc tcatgatgct
      601 gttcggcgcc gtgggctacg ttttcaagaa actcgattac ccgctggcgc cgctggtgct
      661 ggcgctggtg ctgggcgaca aggccgaaga ctcgttccgc caggcgatgc tgttgtcgca
      721 gggcgagctg tcgatcctgt ggtccaatcc gctggtcggc agcatctcca cgctggccct
      781 cgtcctgctg ctgtggccgg tggtgtcgct ggtcatcgcc aggttccggc catcgaaatc
      841 gcgggacttc gtggcggagc agccggtcga ttagcgcctc tctattcatg gtatttgggg
      901 agatcaacga tgccggtaat cgccatgacc caggaaatgg ggtccctcgc caaggacgtt
      961 gccctcgagc ttgcgctcaa gggcaagctc gacgtgatgc gccacgaggt gctggacaac
     1021 gtcgcgcaga agatgcacgt gcccagcagc gtgatcagcc ggttgcgcga aggaagggcc
     1081 ggcctggtgg aacgcctcac caccgacccg cagcgggtgg ccgtctacac cgcggccgag
     1141 gttttcgcac tggcggccaa gggcaacatc gtgctgcgcg gctggggcac gacctgcctg
     1201 ctgcggccgg tgcagcatgt gatcacggtt cgcatcacgc gtccgttcgc gctgcgcgtc
     1261 aagtggctga tggagcacct ggaactcgac gacgagtcgt tcgcggcaac ggaggtccgg
     1321 cgcagcgacg aggcccacgc ggcgaagatc aaggcccagt tcggcgtgac ctggggcgat
     1381 ccgctcctgt acaacatggt gctgaacacc gaccggatgt cggtcgaaac ctgcgcccag
     1441 cagatcctgc atctggcggg ccgtcctgaa ttccaggaaa cggcgcagtc gcgcgccatg
     1501 ctggccgacc tggcattgag cgccgccgtg cgcgccgcgc tgaaggagaa cgagccgacc
     1561 cgcgagacca acatcgagat cgaatcgcag gggggcaagg tgatgctgcg cggcatcgtg
     1621 ctcaacgcgc aggaacgggc cgaagccgcc cgggtggcgg gcacggtggc cggcgtcggc
     1681 gacgtggaca accagctgcg gctgatggtc atcagccggc ggtttactta cgcgaagact
     1741 tgactttcag attttccccc gctgcttcag gcggctgagc gtt
//
LOCUS       DASWIY010000317         2977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_947780, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000317 DASWIY010000000
VERSION     DASWIY010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947780"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(391..810)
                     /locus_tag="VGA59_11505"
     CDS             complement(391..810)
                     /locus_tag="VGA59_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720349.1"
                     /translation="MLRNLSIAVLCLAAGGWIAPAAAQGIYRCGDSYSRQRCPDGKPV
                     QAEDARSAEQARQTSVAAQRDARMADAMEKARLKEEAKPAQAYIPLPKAQNAAQERGK
                     TASGAKAAKPAHFTAVSPKKPGQTQTKKKKKAKKKAA"
     gene            complement(848..1456)
                     /gene="pth"
                     /locus_tag="VGA59_11510"
     CDS             complement(848..1456)
                     /gene="pth"
                     /locus_tag="VGA59_11510"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX9720350.1"
                     /translation="MIKLFVGLGNPGPEYEATRHNVGFWWVDALARELKLTLVAQKGF
                     HGLVARGMLHGQTVWLLEPQTFMNLSGRAVAALSRFYKIAPEDILVVHDELDLVPGQA
                     KLKFGGSHAGHNGLRDIHAQLGTDDYWRLRLGIGHPGAKDEVINWVLKKPAPDQRTAM
                     EECIARTLKAAPALLAGEMDKATLLVHTSKPPRPKPPRPEGV"
     gene            complement(1557..2261)
                     /locus_tag="VGA59_11515"
     CDS             complement(1557..2261)
                     /locus_tag="VGA59_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX9720351.1"
                     /translation="MKFVAYERAKQGTGASRRLRNTGKTPGIVYGGEGQPQLIELDHN
                     ALWHALKKEAFHSTILDMELDGKDAKVLLRDVQVHPYKQQVLHIDFQRVDAKTRLHMK
                     VPLHYVKAEESQAVKFEGCVVNHVMSEIDISCLPADLPEFVEVDLSAMKKGMSLHLNE
                     ITLPKGVTAVTRGKPNPVLVSMVATGGGEEAAEAEVEGAVPAVVAAPVPEVKAPAAGK
                     GDKGDKGDKKAPAKKK"
     gene            complement(2355..>2977)
                     /locus_tag="VGA59_11520"
     CDS             complement(2355..>2977)
                     /locus_tag="VGA59_11520"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX9720352.1"
                     /translation="NLLQTVGVARVLTMDLHADQIQGFFDIPVDNIYASPVLLGDLRH
                     KNYDDLIVVSPDVGGVVRARALAKQLDTELAIIDKRRPRANVSEVMHVIGEIEGRNCV
                     IMDDMIDTAGTLLKAAEVLKERGAKKVYAYCTHAIFSGPAIDRIAKGAVLDEVVVTNT
                     IPLSQAARKCGKIRQLTVAPLIAETIQRIAKGESVMQLFSDQENLF"
BASE COUNT          447 a          982 c          945 g          603 t
ORIGIN      
        1 gccatttcga tgagccgcag tgcgtgcagg tctgccccgt gtcgtgcatc cccgtcaacc
       61 cccagcatgt ggaaagccgc gagacgctgt ggcagaagta ccagcggctc gccgaacagg
      121 cccggcaggc ggccgcgcca gcgggtatgg gcacaaggga cggcggctag aagcttgtca
      181 ttgcgggctg ggctcgcgta acgcccgggg cgcgcagctg tccagtgcag cgagcccaat
      241 gccgggccta cgctatgcac gtaccgaggc atcgcgctcc ggcccggccc cgcaatccat
      301 gactccggaa ttcatggatg ccggatcaag tccggcatga caagagggat gtcattgcgg
      361 gcttgacccg caatccatgt ccctctcagc ttaggccgcc ttcttcttcg ctttcttctt
      421 tttcttcgtc tgggtctggc ccggcttctt cggcgatacg gcggtgaagt gggccggctt
      481 ggcggctttc gcgccgctcg ccgtcttgcc gcgctcctgg gcggcattct gcgctttggg
      541 cagcggaata taggcctgcg cgggcttggc ttcttccttc agccgggctt tttccatcgc
      601 gtcggccatc ctggcgtcgc gttgggcggc aacgctggtc tgcctggcct gctcggcgct
      661 gcgagcgtcc tcggcctgca ccggcttgcc gtcggggcag cgttgccggc tgtagctgtc
      721 gccacagcgg tagatgccct gggcagcggc cggcgctatc cagccgccgg ccgccaggca
      781 cagaacggcg atcgacaaat tcctgagcat ggtttttctc ctcctctttc tctctctgtt
      841 gaaagcgtca gacgccctcg ggccgcggcg gcttgggccg cggtggcttg ctggtgtgca
      901 ccagcagcgt cgccttgtcc atctcaccgg ccaacagcgc gggcgcggcc ttgagcgtgc
      961 gcgcgatgca ctcctccatc gccgttcgct ggtcgggagc cggtttcttg agtacccagt
     1021 tgatgacctc gtccttcgcc cccggatggc cgatgcccag ccgcagccgc cagtaatcgt
     1081 ccgtgcccag ttgcgcatgg atgtcgcgaa gtccgttgtg cccggcgtga ctgccgccga
     1141 acttgagctt ggcctggccc gggacgaggt cgagttcgtc atgcacgacc agtatgtcct
     1201 cgggtgcgat cttgtagaag cgcgagagcg cggccacggc ccggccggag aggttcatga
     1261 aggtctgcgg ctccagcagc cagacggtct ggccgtgcag catgccgcgc gcgaccaacc
     1321 cgtggaagcc cttctgcgcg accagcgtca gcttgagttc gcgcgccagc gcatcgaccc
     1381 accagaaacc cacgttgtgg cgcgtggcct cgtactcggg gcccgggttt cccagtccga
     1441 cgaacagttt gatcattatt aaatggggtc agattccaat ttcgatgatt atcgggctgc
     1501 ccaaatggca aacggcccac cgaagtgagc cgtttaccgc tggctttgtg aagaaattac
     1561 ttcttcttgg cgggcgcctt cttgtcgccc ttgtcgccct tgtcaccctt gccggcggcg
     1621 ggcgccttga cttcggggac cggggcggcg acgacggcag gaaccgcgcc ttcgacttcg
     1681 gcctcggcgg cttcttcacc gccgccggtc gccaccatgg agaccagcac cgggttgggc
     1741 ttgccgcgcg tcacggccgt gacgcccttg ggcagggtga tctcgttcag gtgcagggac
     1801 atgcccttct tcatggcgga gaggtcgacc tcgacgaact cgggcaggtc ggccggcagg
     1861 caggaaatat cgatctccga catcacgtgg ttgaccacgc agccctcgaa cttcacggcc
     1921 tgcgattcct cggccttgac gtagtgcagc ggcaccttca tgtgcagccg ggtcttggcg
     1981 tccacgcgct ggaaatcgat gtgcagcacc tgctgcttgt acgggtgcac ctgcacgtcg
     2041 cgcagcagca ccttggcgtc cttgccgtcc agttccatgt ccaggatggt ggagtggaac
     2101 gcctccttct tcagggcgtg ccacagcgcg ttgtgatcga gttcgatcag ttgcggctgg
     2161 ccttcgcccc cgtagacgat gccgggcgtc ttgcccgtgt tgcgcagacg gcggctcgca
     2221 cccgtaccct gctttgcgcg ctcataagcg acgaatttca tgacttgctc caaaaggagt
     2281 ccaccgcgac cagtgtgact ccggttaaaa aaggcccgct cagcgcgggc cgctcatgcc
     2341 ccggatcagg ctgcctaaaa caggttctcc tgatccgaga acaattgcat caccgactct
     2401 cccttggcaa tgcgctggat cgtctcggcg atcagcggcg ccacggtgag ctggcggatc
     2461 ttgccgcact tgcgtgccgc ttgggacaac gggattgtgt tggtcaccac cacctcgtcc
     2521 agcaccgctc ccttcgcgat gcggtcgatg gcggggcccg agaagatcgc gtgcgtgcaa
     2581 taggcgtaga ccttcttggc tccccgttcc ttcaggacct cggcggcttt cagcagggtg
     2641 cccgcggtgt cgatcatgtc gtccatgatc acgcagttgc ggccctcgat ctcgccgatc
     2701 acatgcatca cttcgctcac gttcgcgcgc ggacgccgct tgtcgatgat ggccagctcg
     2761 gtatcgagct gcttggccag cgcgcgggca cgcaccacgc cgcccacgtc gggcgacacg
     2821 acgatcaggt cgtcgtagtt cttgtggcgc aggtcgccca gcagcaccgg cgaagcgtag
     2881 atgttgtcca ccggaatgtc gaaaaagccc tggatctggt cggcgtgcag gtccatggtc
     2941 agcacgcggg ccacgcccac cgtctgcagc aggttgg
//
LOCUS       DASWIY010000318         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2075922, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000318 DASWIY010000000
VERSION     DASWIY010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075922"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="VGA59_11525"
     CDS             <1..1452
                     /locus_tag="VGA59_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9720353.1"
                     /translation="TTRKFGGTGLGLAICKRLAELMGGEVGVESEPGKGSTFWFSARL
                     GIGTTTQRRLVPSCDLRGRRALVVDDNDHARAVIMDMLERMTFLASEVASGAAAVDEV
                     RRAASTGHPYDVVYLDWRMPGMDGMDTARRIKSLGLAQPPILMMVTAYSREEMLKEAR
                     SIGIDNVLVKPVNASLLFDTTMSVLSTVQRAAPQTGAPPEPDSARTRLAAIRGARVLL
                     VEDNDINQQVARELLEDAGLVVDVADNGQIALDMVQRASYDLVFMDMQMPVMDGVTAT
                     RQIRKIGWLARLPIVAMTANAMEQDRRRCIEAGMNDAVIKPIDPQGLWDMLLRWVRLD
                     EPAPAADPGPPSGTQRASAKPDASSVDGLPQDIAGLDTPLGLSRMLGKKSLYVAMLRR
                     YATGQKGVCEQIHQALAMGDMPTAERLAHTTKGVSGNIGATGIQNLAGALEQSLKEYQ
                     PPLDVQQRLRELEGPLAALVEALEYQWGAEQPA"
     gene            complement(1458..1847)
                     /locus_tag="VGA59_11530"
     CDS             complement(1458..1847)
                     /locus_tag="VGA59_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720354.1"
                     /translation="MQGESTSTGPGPKLSLSLAWVTGRQILRCVCEGQADIEESARLL
                     KMAADEPTPPGSALLVNLLGVTGNLALNERFELGVVTVKALSKFRRVAIVQEARPDDG
                     FGALVARNRGANIAVFRTENDAMNWLS"
     gene            2239..>2781
                     /locus_tag="VGA59_11535"
     CDS             2239..>2781
                     /locus_tag="VGA59_11535"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEX9720355.1"
                     /translation="MDTAAVALADYYEDKFAVMLARSPVAGGDTVKLGRNPLPGKPGP
                     GNGLGLHATTDGSLADVWHWKSVRSDAGHQFDDNYFGPPMEATAGKRYTGGYTQDPKT
                     GGGFEENFKKIKDSKFVKVPYLPKDLAAQQARMGRFDPSPNVSDMGEFSMARDDVVPW
                     TAELDASIPVGTVIPSVIVDK"
BASE COUNT          529 a          887 c          909 g          456 t
ORIGIN      
        1 accacgcgca agttcggcgg caccgggctg ggcctggcga tctgcaagcg gctggccgag
       61 ctcatgggcg gcgaggtggg cgttgaaagc gaacccggca agggcagcac cttctggttc
      121 agcgcccggc tgggcatagg caccaccacc cagcggcgac tggtaccgag ctgcgacctg
      181 cgcggccggc gcgccctggt ggtggacgac aacgaccacg cgcgcgccgt gatcatggac
      241 atgctcgagc gcatgacgtt cctggccagc gaagtcgctt cgggtgcggc cgcggtcgat
      301 gaggtgcgca gggccgcgag taccgggcat ccttacgacg tggtctacct cgactggcgc
      361 atgccgggca tggacgggat ggacacggca cggcgcatca agtcgctggg gctcgcgcag
      421 ccgcccatcc tgatgatggt caccgcctac agccgcgagg agatgctcaa ggaggcccgc
      481 tccatcggca tcgacaacgt gctggtcaag ccggtgaacg cctcgctcct gttcgacacc
      541 accatgtcgg tcctcagtac ggttcagcgt gccgcgccgc aaaccggagc cccgcccgag
      601 cccgactcgg ctcgcacgcg gctggccgcg atccggggcg cgcgcgtcct gctcgtggaa
      661 gacaacgaca tcaaccagca ggtggcgcgg gagctgctcg aagatgcggg gctggtcgtg
      721 gacgtcgccg acaacggcca gatcgccctg gacatggtcc agcgcgcttc ctacgacctg
      781 gtcttcatgg acatgcagat gccggtgatg gacggggtca ccgccacccg ccagatccgc
      841 aagatcggct ggctggcgcg gttgccgatc gtcgcgatga cggccaacgc catggagcag
      901 gaccggcgca ggtgcatcga ggccggcatg aacgatgcgg tgatcaagcc gatcgatccg
      961 cagggcctgt gggacatgct gctgcgctgg gtccgcctgg acgagccggc gccggcagcg
     1021 gaccccggcc cgccgtccgg aactcagcgc gcatcggcaa agccggacgc ttcatccgtg
     1081 gacggcctgc cgcaggacat cgccgggctg gacaccccgc ttggcctgag ccgcatgctc
     1141 ggcaagaagt ccttgtacgt ggccatgctg cggcgctatg cgaccggcca gaaaggggtt
     1201 tgcgaacaga ttcaccaggc actggccatg ggcgacatgc ccactgccga gcggctggcc
     1261 cacacgacca agggcgtgtc cggcaacatc ggcgccaccg gaatacagaa cctggccggc
     1321 gcattggaac agtcgctcaa ggagtaccag ccgccgctgg acgtgcagca gcggctgcga
     1381 gaactggaag gcccgctggc cgcgctggtc gaagcgctgg aatatcaatg gggcgccgaa
     1441 cagcctgcct agcctgccta gctcagccag ttcatcgcat cgttttcggt acggaacacg
     1501 gcgatgttgg cgccgcggtt gcgcgccacc agcgccccga agccgtcgtc cggcctggcc
     1561 tcctgcacga tggccacccg gcggaatttc gaaagcgcct tgactgtcac aacaccgagc
     1621 tcgaagcgtt cgttaagggc caggttcccc gtcacgccaa gcagattcac cagcaaagca
     1681 ctgccgggag gggttggttc gtcggcagcc atcttcaaca ggcgtgccga ttcctcgatg
     1741 tccgcctgcc cttcacagac gcagcgaagt atttgtcttc cggttaccca agccagactc
     1801 agggacagtt tgggacccgg gccggtgctg gtggattcgc cttgcatgga agattgctcc
     1861 ttgacgccag ttgaattgtg gcgcaagcgt atccgtgctg ggcagttagt gcgaggagtc
     1921 ccagttattg ctgtggactt gtcgcacgac gccgatgacg gcaaacgcca tggagtcgcc
     1981 attcagtgca tcgtccgcaa aaatcacgtt ctaaaacaaa atcattcacg cgaatgcggc
     2041 tcctggactt ttgtaagcgc tcgcgtaacg caacaggaag tcgggtgtgg ttaatcagtg
     2101 acggcatgcg cagtaaatgg taacggccac attgatgcgc tgcagcattt ggaaccctat
     2161 ttctcccagc agaccatttg ccaatattgg cccttgtccg gccccgcttg agctcaagca
     2221 acgctacaag tccggatttt ggatacggcg gcagtcgctc ttgccgacta ctacgaagac
     2281 aagttcgccg taatgctggc ccgctcgccc gttgctggcg gagacaccgt caagctcggc
     2341 cgcaatccct tgccgggcaa gcccggaccg ggcaacggcc tgggccttca tgccaccacc
     2401 gacggcagcc tggctgacgt gtggcactgg aagagcgtgc gaagcgacgc gggccatcag
     2461 ttcgacgaca actatttcgg gccgcccatg gaggccacgg cggggaaacg atacaccggc
     2521 ggctacacgc aggatcccaa gaccggcggc gggttcgagg aaaacttcaa gaaaatcaag
     2581 gacagcaagt ttgtcaaggt gccgtacctt ccgaaggacc tggcggcgca gcaggcgcgc
     2641 atgggccggt tcgacccgag cccgaacgtg agcgacatgg gcgagttttc gatggcgcgc
     2701 gatgacgtcg tgccatggac tgccgagctc gatgcaagca tcccggtggg caccgtgatc
     2761 ccgtcggtga tcgtcgacaa g
//
LOCUS       DASWIY010000319         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2450663, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000319 DASWIY010000000
VERSION     DASWIY010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2450663"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1747
                     /locus_tag="VGA59_11540"
     CDS             125..1747
                     /locus_tag="VGA59_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX9720356.1"
                     /translation="MTDVNKKELAGEIARRRTFAIISHPDAGKTTLTEKLLLFSGAIQ
                     MAGAVKAKKAVRHATSDWMKLEQERGISVTSSVMQFEYHGRVVNLLDTPGHADFSEDT
                     YRTLTAVDSALMVIDCAKGVEERTIRLMEVCRLRQTPIFTFINKLDREGREPVELLDE
                     VESVLGIQCAPITWPIGMGKRLKGVYHLLDDAVHLYESGKNYVTQQAEIIPGIDNPLL
                     DQRLGQEMGMGAAELREEIELVRGASHDFDSCAYLAGEQTPVFFGSAINNFGVTPLLD
                     AFVEHAPPPGSRETVGRAVQADETALTGFIFKIQANMDPAHRDRVAFMRICSGRFESA
                     MKMYHVRLGKTVKAANALTFMAQEREHVESAWPGDIVGLHNHGTISIGDTFSEGETLQ
                     FTGIPHFAPELFRRAQLRDPLKLKALQKGLAQLSEEGATQFFRPLASNDLILGAVGIL
                     QFDVVAYRLQNEYGVEAVFENVNVQTARWVDCDDARKLADFTRQLQNNLAEDGAGQLV
                     YLAPTRVNLQLTQERWPEVRFKATRETTIFAG"
     gene            1751..3091
                     /locus_tag="VGA59_11545"
     CDS             1751..3091
                     /locus_tag="VGA59_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9720357.1"
                     /translation="MNLLHFPDPERRRRVWAIALPITGGMMSQNLLNLVDIGMVGHLG
                     DSALAATGIGSFSNYLAISFIIGLSAGVQALAARRLGEGRVDETAVPLNGGLMLAAII
                     GLPLCILFILGTPLAFTFLTDDPAVREQGIPYLQVRIASMIAVGMNFSFRGYWSAIHM
                     TGVYLRTLLIMHAINIFLNWVLIFGNLGAPELGVFGAGLATTISLYIGTLLYFFFALR
                     HARDKGFLHRRPSRQTLWQQFRLSLPSSLQQLFFAGGMVALIWIVGHIGTAEVAAVNV
                     LMTFHITAILPAFGVALATMTLVGNALGRGDAEDAAAWGWNGAALTFVYGAALSLLLI
                     PLADPILGVFLKNPETRQLAYLPMVLWALMISVDSAGMVLMNALIGAGDTRRSMWISI
                     VWQWLFYLPLAWLVGPVLGFGLLGVWIVNVLYRVGQSVNCVTQWRGRKWAQIQI"
BASE COUNT          536 a         1059 c         1011 g          566 t
ORIGIN      
        1 gaacccgcag cgctagcgcg cgcggtgcat agcctgcttc tctgttagaa tggcgggctt
       61 tttcgccttt gccgactttg tccatttcct tgaactgcca cagcccagtc accgcagccc
      121 agtcatgacc gatgtgaaca agaaagaact tgccggggaa atcgcccgcc gccggacctt
      181 tgccatcatt tcccacccgg acgcgggcaa gaccacgctg accgaaaagc tgctgctgtt
      241 cagcggcgcg atccagatgg ccggtgcggt caaagccaag aaggccgtgc gccacgcgac
      301 ctccgactgg atgaagctgg agcaggagcg cggcatctcg gtcacctcct cggtgatgca
      361 gttcgaatac cacggacggg tggtcaacct gctggacacg cccggccacg ccgatttctc
      421 cgaggatacc taccgcacgc tgaccgcggt cgactcggcc ctgatggtga tcgactgcgc
      481 caagggcgtc gaagagcgca ccatccggct gatggaagtc tgccggctgc ggcagacccc
      541 gattttcacc ttcattaaca agctggaccg cgaaggccgc gagcccgtcg agttgctcga
      601 cgaagtggag tccgtgctgg gcatccagtg cgcgccgatc acctggccca tcggcatggg
      661 caagcggctc aagggggtct accacctgct ggatgacgcc gtgcacctgt acgaatcggg
      721 caagaactac gtgacccagc aggccgagat catcccgggc atcgacaacc cgctgttgga
      781 ccagcggctg ggccaggaga tgggaatggg cgcggccgaa ctgcgcgaag agatcgagct
      841 ggtgcgcggc gccagccacg atttcgattc gtgcgcttac ctggcgggcg agcagacgcc
      901 ggtgttcttc ggctccgcca tcaacaactt cggcgtcacg ccgctgctgg acgctttcgt
      961 cgagcacgcg ccgccgccgg gctctcgcga aaccgtcggc cgggccgtac aggccgacga
     1021 aactgccctg accggcttca ttttcaagat tcaggccaac atggacccgg cccaccgcga
     1081 ccgggtcgcc ttcatgcgca tctgctcggg ccgcttcgaa tccgccatga agatgtacca
     1141 cgtgcggctg ggcaagaccg tcaaggcggc caacgcgctg accttcatgg cgcaggagcg
     1201 cgagcacgtg gagagcgcct ggccgggcga catcgtgggc ctgcacaatc acggcaccat
     1261 cagcatcggc gataccttca gcgagggcga aacgctgcaa ttcaccggca ttccgcactt
     1321 cgcgcccgag ctgttccgcc gcgcgcagct gcgcgacccg ctcaagctca aggccctgca
     1381 gaagggcctg gcccaactgt ccgaggaagg cgccacgcag ttcttccggc cgctggccag
     1441 caacgatctg atcctgggcg cggtgggcat cctgcagttc gacgtggtcg cctaccgcct
     1501 gcaaaacgag tacggcgtgg aagccgtgtt tgaaaacgtc aacgtgcaga ccgcccgctg
     1561 ggtcgactgc gacgacgcca ggaagctggc cgatttcacc cggcagctgc agaacaacct
     1621 ggcggaggac ggcgcgggcc aactcgtgta cctggcgcca acccgcgtca acctgcagct
     1681 gacccaggaa cgctggccgg aggttcgctt caaggccacc cgggaaacca cgatttttgc
     1741 cggctgagcc atgaacctgc tgcatttccc ggaccccgaa cgccgccggc gggtctgggc
     1801 cattgcgctg ccgatcacgg gcggcatgat gtcgcagaac ctgctgaacc tggtggacat
     1861 cggcatggtc gggcacctgg gggacagtgc gctggcggca accgggatcg gcagtttcag
     1921 caactacctc gcgatctcgt tcatcatcgg cctgtcggcg ggcgtgcagg ccctggccgc
     1981 tcggcggctg ggtgaggggc gtgtcgatga aacggctgtt ccactgaacg gcggcctgat
     2041 gctggcggcc atcatcggcc tgccgctgtg catcctgttc atactgggca cgccgctggc
     2101 ctttaccttc ctgaccgacg accccgccgt gcgcgagcag ggcatcccct acctgcaggt
     2161 gcgcatcgcc agcatgattg cggtcggcat gaacttcagt tttcgcggct actggagcgc
     2221 cattcacatg acgggcgtct acctgcgcac gctgctgatc atgcacgcga tcaacatttt
     2281 cctgaactgg gtactgatct ttggcaattt gggcgcccct gagctgggcg tgttcggcgc
     2341 cggcctggcg actacgatct cgctgtacat cggcaccctg ctgtattttt tctttgccct
     2401 ccggcacgcc cgggacaagg gcttcctgca ccgccggccg tcccgccaga ccctgtggca
     2461 gcagttccgg ctgtcgctgc ccagcagcct gcagcagctg ttcttcgccg gcgggatggt
     2521 cgcgctgatc tggatcgtcg gccacatcgg caccgccgaa gtggcggcag tcaacgtcct
     2581 gatgaccttt cacatcacgg ccatcctgcc ggccttcggc gttgcgctgg ccaccatgac
     2641 gctggtcggc aatgccctgg gccgcggcga cgccgaagac gcggcggcct ggggctggaa
     2701 cggcgccgct ttgacgttcg tctatggcgc cgcgctgagc ctgctgctga tcccgctcgc
     2761 cgacccgatc ctgggcgtgt tcctgaagaa cccggagacg cggcagctcg cttacctgcc
     2821 gatggtgctg tgggccctga tgatcagcgt ggacagcgcc ggcatggtcc tgatgaacgc
     2881 cctgatcggc gccggcgaca cgcgccgcag catgtggatc agcatcgtgt ggcagtggct
     2941 gttctatcta ccgctggcct ggctggtcgg cccggtactg ggctttggcc tgctcggcgt
     3001 atggatcgtc aacgtcctct accgcgtcgg ccagtcggtc aactgcgtca cgcagtggcg
     3061 cggccgcaag tgggcgcaga tccagatctg agcgcttgaa atgcggtcgc accggacgca
     3121 gattcagagt cagtcgcggg tgttgaccgc gcccgcccga ttctcgccgg ac
//
LOCUS       DASWIY010000320         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2450791, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000320 DASWIY010000000
VERSION     DASWIY010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2450791"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..759
                     /locus_tag="VGA59_11550"
     CDS             244..759
                     /locus_tag="VGA59_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9720358.1"
                     /translation="MSGSPPPIDIRPASVGDVALILRFIKELAAYERAEQEVVATEAS
                     LRASLFGEGARAHALICRVAGVEAGFAVYFFNYSTWQGRQGLYLEDLYVSPAHRKAGA
                     GKAMLQHLARIAVKKRCGRFEWSVLDWNEPAIAFYRSIGAVAMDEWVRYRLAGEALAN
                     FAGGSGPGAAA"
     gene            complement(913..3573)
                     /locus_tag="VGA59_11555"
     CDS             complement(913..3573)
                     /locus_tag="VGA59_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEX9720359.1"
                     /translation="MSIRNLEALFQPRSIAVIGASDRPGSVGSVVLRNLKSGGFYGVI
                     WPVNRRHAMVDAAPAWLDVASLPGVPDLAVICTPAPTVPDLIAELGRKGVRAAIVMTA
                     GLKQSVGEGGTLEQAMLAAAQPYLLRILGPNCIGLLVPGIGLNASFAPGNALPGKLAF
                     VTQSGALATAMLDWANGRGIGFSHFISLGDSADVDFGDALDYLGSDSGTRAILMYAES
                     LQAGRKFMSAARAAARNKPVIVVKAGRAPDGARAAASHTGALAGSDAVFDAVVHRAGM
                     LRVDTIEALFDAAETLAHPHPQRGEYLAITTNGGGAGVLASDALALGGGKLAQLTPAS
                     MAALDQCLPANWSRGNPVDLTAESPVQRYQDALRILLAAPEVDSVLFVHAPTALVPAA
                     DIAAACLPLVERAQKPVLACWLGGPTVAAARASWSAAGIACFNTPERAVSAWLQLVMH
                     ARNQLALQQIPPASLIEFTPQRQRAQALIDKVLREGREWLDEVQAMELLQAYGIPVAD
                     TRRAHDAEQAVEQAVQIGFPVALKIISPQTVHKSDAGGVVLNLASADEVRAAAVKMKE
                     LVARQQPARMRGFAVQAMARRSRELIVGLSNDRVFGPVMLCGSGGTQVELNKQHAVAL
                     PPLNMLLARDLIERSGVAPLLGIWRGRPAANEQALLETLLKVSQMACDLTGIAELDIN
                     PLLVDAQGVLALDSRVRVRDHRVLAPLAIRPYPQELSEPLQLAGSALLLRPIRPEDGE
                     RLKLFYQKAAPADMRLRFFMSRRELPPSELARYSQIDYDREMTFVALEPADAQGRQEM
                     VGEVRAVCDPDNRTAEFAIQVASEWQARGLGRRLMERLVQYLREHGTAEVVGECLPEN
                     ASMAALAKSLGFAVTPGPPGESTALRLALR"
BASE COUNT          646 a         1187 c         1180 g          616 t
ORIGIN      
        1 ttgtatgagt tggagattca cgctggcgaa gatgtcgctg tcgcgtatgg cttgcttgcg
       61 atcgccggag cgcctgcgcg gtgcaggctc acggttggac tacggcgaat tggacgcact
      121 tgggcagtct ttcatgaaca ccactcgatg cccatcgatc ttctacagta atacgcccgc
      181 tgagatcttc gcgtctgctt gcggccagga gcagccctca cgacgatccg acataatttc
      241 cgcatgtctg gctcgccgcc cccaatcgac atccgtccgg ccagcgtcgg cgatgttgcg
      301 ctgatcctcc gtttcatcaa ggaactggcc gcttacgaaa gagcggagca ggaagtggtc
      361 gctaccgagg cgagtctccg cgcgtcgcta ttcggggaag gggcccgtgc acatgccctg
      421 atctgccgtg tcgcgggtgt ggaagccgga ttcgccgtct acttcttcaa ctactccacg
      481 tggcaaggca ggcaaggcct ctacctcgag gacttgtacg tgtcgccggc ccacaggaag
      541 gcaggtgcgg gaaaggcgat gctgcaacac ctggcgcgta tagcagtgaa aaagcgctgc
      601 ggccgcttcg agtggagtgt gctcgactgg aacgagcctg ccatcgcctt ctacaggtca
      661 atcggtgccg tcgctatgga cgaatgggtg cggtaccgcc tggccggcga ggcgctagcg
      721 aacttcgccg gaggcagcgg cccgggtgct gccgcgtagc cctcggcaag gagcagaggc
      781 cgactcgcgc tggtgtgacg tttcgcccta tggctgctct gggccgaggc cgaatccgaa
      841 gcgaccagat ttcgtttgtt gccggcctga attcgactcg caggctaacc cgtgctgatt
      901 ccgtgaagca gctcagcgca gcgcgagacg cagcgccgtg gactctcccg ggggccccgg
      961 agtcaccgcg aaacccaggg acttggccaa cgccgccatg gatgcgttct caggaaggca
     1021 ctcgcccacg acttcagcgg tcccatgctc acgcaggtac tgcaccagtc gctccatcag
     1081 tcttcttccc aggcctcttg cctgccattc ggacgccacc tggatcgcga actccgccgt
     1141 acgattgtcc gggtcgcaca ccgcacggac ttcgcccacc atttcctgcc ggccctgagc
     1201 gtccgcaggt tcgagcgcga cgaatgtcat ctcccggtcg tagtcgatct ggctgtatct
     1261 ggcgagttcg gatgggggca actcgcggcg ggacatgaag aacctcaatc gcatatcggc
     1321 gggtgccgcc ttttggtaga acagcttgag gcgttcgccg tcttcggggc gaatcggtcg
     1381 cagcagcagc gcgcttcccg ccagttgcaa gggttcgctc agttcctgcg gataggggcg
     1441 tatcgccaac ggcgccaaga ccctgtggtc gcgaacccgc accctcgagt ccagggcaag
     1501 cacaccttgc gcatccacca gcaagggatt gatgtcgagt tcggcgatgc cggtgaggtc
     1561 gcaagccatc tgcgaaacct tcaacagggt ctccagcagc gcttgctcgt tggcggccgg
     1621 ccggccgcgc caaatcccca gcagaggcgc aacaccgctg cgctcgatca gatcacgcgc
     1681 gagcagcata ttcagcggag gcaaagccac cgcgtgctgc ttgttcaatt cgacttgggt
     1741 gccaccgctg ccgcaaagca tgaccggccc gaacacgcga tcgttggaca ggcccacgat
     1801 gagttcgcga gaacgtcgcg ccatcgcctg aactgcaaag ccccgcatcc tggcgggctg
     1861 ctgccgtgcc accagttcct tcatcttgac cgcggcggct cgcacctcgt cggcggaagc
     1921 cagattcagg accacgccgc cggcatcgga cttgtgaacg gtctggggcg agatgatttt
     1981 caatgccacc gggaaaccga tttgcacggc ctgctcgacc gcttgctcgg catcatgggc
     2041 cctgcgcgtg tccgcgacag ggatgccata cgcctggagc aactccatag cctgcacttc
     2101 atccagccat tcgcgacctt cgcgaagtac cttgtcgatc agcgcttgcg cgcgctggcg
     2161 ctgcggcgtg aattcgatca acgaggcagg cggaatctgc tgcagggcga gctgattgcg
     2221 cgcatgcatg accagttgaa gccaggccga cacggcgcgt tcgggcgtgt tgaagcaggc
     2281 aatgcccgcc gcggaccagg aggcccgggc ggccgcgacc gtgggtccgc ccagccagca
     2341 ggccaatacc ggcttttgcg cacgctccac cagcggcagg cacgcggccg cgatgtcggc
     2401 ggccggcacc agggccgtcg gcgcatgaac gaagaggact gaatcgactt cgggcgcggc
     2461 aagcaggatg cgcaaggcgt cctggtagcg ctgcaccggt gattcggcgg tcaggtccac
     2521 cgggttgccg cgcgaccagt tcgccggcag gcactggtcg agggcggcca tcgacgccgg
     2581 ggtcaattgc gccagtttcc cgccaccgag cgcaagggcg tcggaagcaa gtacgcccgc
     2641 tccaccgcca ttggtcgtga tggcaaggta ttcgccgcgc tgcgggtgcg ggtgcgccag
     2701 tgtttcggcg gcgtcgaaca gcgcctcgat cgtgtcgacc cgcagcatgc ccgcccgatg
     2761 gaccaccgcg tcgaagaccg catcggagcc ggccagggcg ccggtgtgcg aagccgcggc
     2821 ccgggcacca tctggcgcgc gtccggcctt gaccacgatc accggcttgt tgcgggccgc
     2881 ggcacgcgcg gccgacatga acttgcgccc agcctgcaac gattccgcgt acatcaggat
     2941 ggcgcgggtg cccgagtcgc tgcccagata gtccagtgca tccccaaaat ccacatcggc
     3001 gctgtcaccg agcgatatga agtgcgagaa gccgatgcct cggccgttgg cccagtccag
     3061 catggccgtg gccaaggcgc ccgattgcgt gacaaaggcc agcttgccgg gcaaggcatt
     3121 gccgggagca aaactcgcgt tcaggccgat accgggcacc agcaggccga tgcagttcgg
     3181 gccgagaata cgcagcagat aaggctgggc agcagcgagc atggcctgct ccagcgtccc
     3241 cccctcgccc accgactgct tgaggccggc ggtcatcacg atagcggccc gcacaccttt
     3301 gcggccgagt tccgcgatga ggtcgggcac ggtgggggca ggcgtacaga tgaccgccag
     3361 gtcgggcaca cccggcagcg acgccacatc gagccaggcg ggggcggcgt ccaccatggc
     3421 atggcgccgg ttgaccggcc atatcacgcc ataaaaaccg ccgctcttga ggttgcgcag
     3481 cacgaccgac ccgacgctac ccgggcggtc ggaggcgccg atcacagcga tcgagcgcgg
     3541 ctggaagagg gcttcgagat tgcggatgct catggctgta gggccggcga gaaattctct
     3601 ggcgcggacc ttttctttat agccgccga
//
LOCUS       DASWIY010000321         4096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2451860, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000321 DASWIY010000000
VERSION     DASWIY010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2451860"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1023)
                     /locus_tag="VGA59_11560"
     CDS             complement(256..1023)
                     /locus_tag="VGA59_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9720360.1"
                     /translation="MTTEILTHVERGVMTITLNRLDRKNAITSAMYGAMADALAAAEN
                     DVNVRVALVQGHETVFSAGNDIGDFLNRPPAGMDSPVFRFLQGIATFTKPLLAAVCGP
                     AVGIGTTMLFHCDLVYAGDNAAFSMPFVNLGVCPEAASSLLVPQMLGYHRAAEALLLG
                     EPFMAEAALEVGLVNRIVPPTEANAVAQSVAQKLAAKPIASLVETKRLMKKGQQQLVL
                     RQMADEGQSFSRLLAEPAAREAFGAFMEKRTPDFSKL"
     gene            complement(1020..1619)
                     /locus_tag="VGA59_11565"
     CDS             complement(1020..1619)
                     /locus_tag="VGA59_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX9720361.1"
                     /translation="MSQSVEFFFDVGSPASYLAWCRLPRICEEAGAALEYRPMLLGGV
                     FQATGNQSPTTIPAKGQYIMADLARFAQRDRIPFIHNPFFPINTLTLMRGAAGLQMRD
                     PSRLVPYVDAVYRAIWVEGRNMNEPAIVAAVLQQAGFEPQTLPALAADPEVKERLKTV
                     TQEAVARGVFGAPTFFVGEQMYWGQDRLDFVKQALEDKT"
     gene            complement(1742..2476)
                     /locus_tag="VGA59_11570"
     CDS             complement(1742..2476)
                     /locus_tag="VGA59_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9720362.1"
                     /translation="MSPVALVIGAGDATGGAIARRFARGGYTVCATRRSLDKLQPLID
                     RIEADGGKAHGFASDARKEDEVAALVERIESTVGPIEVLVFNIGANAPSSILDETARR
                     YLKIWEMACLGGFLNGREVARRMVAREGEGHKGTIIFTGATASLRGSANFAAFAGAKH
                     ALRALAQSMARELGPRGIHVAHVIIDGAIDTAFIRDNFPERYVLKERDGILNPDHIAD
                     AYWMLHRQPRDAWTHELDLRPWMEKF"
     gene            complement(2484..3287)
                     /locus_tag="VGA59_11575"
     CDS             complement(2484..3287)
                     /locus_tag="VGA59_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9720363.1"
                     /translation="MHSFDAAIALAPHGEGAWQGHTSAAYANMIGPFGGVTAAQALNA
                     VLRHRERLGEPIAFTVNFAAALADGPFLVLARPARTNRSTQHWIVEMRQGERTVLTAT
                     AVTALRRDTWGATEMTMPHVPGAQEVPPPTRRARVEWINRYDMRFIHGGLPPAWDGAE
                     GHNSRTQLWVRDNPPRPLDFASLTATADVFFPRIWRRRATMTPIGTVSMTLYFHAGEA
                     QLQATGAGYLLGQAEAQAFRNGFFDQTAQLWNEAGDLLATSHQVVYYKE"
     gene            complement(3362..>4096)
                     /locus_tag="VGA59_11580"
     CDS             complement(3362..>4096)
                     /locus_tag="VGA59_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9720364.1"
                     /translation="AQWLAGQGWRVVTFDYRGSGETGPSGRALRGFKADLFDWTRDYE
                     AAIDHAHAALPDRPLYLLGHSLGAQLPGLLNNQAKVSGMLSVAAGSGYWRENAPQLRR
                     IVFYFWFVLVPLATWLCGYFPGRSLRKVGDLPAGVVMQWRKWCLNPRYSVGAEGEAVR
                     QRYASAKFPVQALSITDDELMTLRGTHSLIDLYENAPSEVRRVAPGDLGVRRLGHFGP
                     FRSEHEARLWPRMAEWIGALARPKAA"
BASE COUNT          664 a         1365 c         1375 g          692 t
ORIGIN      
        1 cggctctctc cgtatacctg gacatccaga agaggtgatc ggcggttctt gaaagcatgt
       61 tcgttctccg ggttcccttg tcttgtcatt gcgggctggg ctcgcggaac gcccggggcg
      121 cgcagctgtc cagtgcagcg agcccaatgc cgggcctaca ctatgcacgt accgaggcat
      181 gacgctccgg cccggccccg caatccatgg atcccgggtc aagcccggga tgacatactt
      241 tggtggtgga tgacttcaga gcttcgaaaa atccggcgtg cgcttctcca tgaacgcgcc
      301 gaacgcttcc ctggccgcgg gctcggccag caaccgcgag aagctctggc cctcatcggc
      361 catctgccgc agcacgagtt gctgctggcc ctttttcatg aggcgcttgg tttccaccag
      421 cgatgcgatc ggcttggccg cgagcttttg cgccacggac tgggcgacgg cgttcgcctc
      481 cgtgggcggg acgatgcggt tgacgaggcc gacttccagg gcggcttccg ccatgaacgg
      541 ctcgcccagc agcagcgctt cggcggcgcg gtggtagccc agcatctgcg gcaccagcag
      601 gctcgaagcg gcttccggac acacgcccag attgacgaag ggcatggaga atgccgcgtt
      661 gtcgccggca tagaccaggt cgcagtggaa cagcatcgtg gtgccaatgc ccaccgcggg
      721 cccgcaaacg gcggccaaca agggcttggt gaacgtggcg atgccttgca ggaagcggaa
      781 gaccggggaa tccatgccag cgggtggccg gttcaggaag tccccgatgt cgttgccggc
      841 actgaacacg gtttcgtgac cttgcaccaa ggcgacacgg acgttcacat cgttttccgc
      901 agcggccagt gcgtcggcca tcgccccgta catcgcggac gtgatcgcgt tcttgcggtc
      961 gagccggttg agggtgatcg tcatcacccc acgctcgacg tgggtgagta tctcggtggt
     1021 catgtcttgt cttccaatgc ttgcttgaca aaatcgagcc ggtcctggcc ccagtacatt
     1081 tgttctccga cgaagaacgt cggcgcgccg aacacgccgc gcgccacggc ttcctgcgtc
     1141 accgtcttca ggcgctcctt gacttcggga tcggcagcca gcgccggcaa ggtctgcggc
     1201 tcgaaccccg cctgctgcag gacggcggct acgatcgcag gctcgttcat gttcctgcct
     1261 tcgacccaga tcgcacggta gaccgcgtcg acatagggca ccagccgcga cggatcgcgc
     1321 atctgcaggc cggccgcgcc gcgcatcagc gtgagcgtat tgatcggaaa gaacgggttg
     1381 tgaatgaacg ggatgcgatc gcgctgcgcg aaccgcgcca ggtcggccat gatgtattgg
     1441 cccttggccg ggatggtcgt gggcgactgg ttgcccgtgg cctggaacac gccgcccagc
     1501 agcatgggcc tgtattccag cgcggccccc gcttcttcgc aaatacgcgg cagccggcac
     1561 caggccaggt aggacgccgg gctgccgacg tcgaaaaaga actcaacgga ctggctcatg
     1621 atctcctctt ggttgtcatg ccggaccccg gatcaagtcc ggggcaggct tgatccggca
     1681 tccatggatt gcgggtcaag cccgcaatga cagacccggt tggcttcccc gggaccttac
     1741 tctagaactt ctccatccac ggccgcagat cgagttcgtg ggtccaggcg tcgcgcggct
     1801 ggcggtggag catccagtac gcgtcggcga tgtggtcggg attgaggatg ccgtcacgct
     1861 ccttgagcac ataacgctcg ggaaagttgt cgcggatgaa ggccgtatcg atggcgccgt
     1921 cgatgatcac atgagcgacg tggatgccgc gcgggccgag ctccctcgcc atgctctggg
     1981 ccagcgcccg cagcgcatgc ttggcgccgg caaaggcggc gaagttggcc gatccgcgca
     2041 acgaagccgt ggcgccggtg aagatgatgg tgcccttgtg gccctcgccc tcgcgcgcca
     2101 ccatgcgcct ggcgacttcg cggccgttga gaaagccgcc caggcacgcc atctcccaga
     2161 tcttcaaata ccggcgcgcg gtctcgtcga ggatgctgct gggcgcgttg gcgccgatgt
     2221 tgaacaccag cacttcgatc ggcccgacgg tggactcgat ccgctcgacc agcgcggcca
     2281 cctcgtcctc cttgcgggcg tcggacgcga agccatgcgc cttgccccca tcggcttcga
     2341 tccggtcgat caggggctgg agcttgtcca ggctgcggcg cgtcgcgcac acggtgtagc
     2401 cgccgcgcgc gaagcgccgc gcgatcgcac cgccggttgc gtcgccggcg cctatcacca
     2461 aagccacggg agacatcaag ccctcactcc ttgtagtaga cgacctggtg ggacgtggcc
     2521 agcaggtcac ccgcctcgtt ccacagctgg gccgtctggt cgaaaaaacc gttgcgaaac
     2581 gcctgggcct cggcttggcc cagcaggtag ccggcgccgg tggcttgcag ctgcgcctcg
     2641 ccggcgtgga agtagagggt catggacacg gtgcctatcg gcgtcatggt ggcgcggcgg
     2701 cgccagatgc gcggaaagaa cacgtccgct gtcgccgtga gcgaggcgaa gtccagcggc
     2761 cgcggcgggt tgtcgcgcac ccagagctgc gtgcggctgt tgtgcccctc cgcgccgtcc
     2821 catgccgggg gcaaccctcc gtggatgaaa cgcatgtcgt atcgattgat ccattccacg
     2881 cgggcgcgac gtgtgggtgg cggcacctct tgcgcgcccg gcacgtgagg catcgtcatt
     2941 tcggtcgcgc cccaggtgtc gcgccgcaag gcagtgaccg cggtcgccgt gagcaccgtg
     3001 cgttcgccct gccgcatctc cacgatccag tgctgggtcg agcggttggt gcgggccggt
     3061 cgggcgagga cgagaaaagg accgtcggcg agcgccgcgg cgaaattgac ggtgaacgcg
     3121 atcggctcgc ccaggcgctc gcgatgccgc agcaccgcgt tgagagcctg ggccgcggtg
     3181 acgccgccga acggcccgat catgttggcg taggccgcgc tggtgtggcc ctgccacgcc
     3241 ccttcgccat ggggggcgag ggcgatcgcg gcgtcgaacg aatgcatgcg atccggcccg
     3301 ctcatacgta aaacctccgc gccacgcgag gcggcatccg gttcggaagc gatctgccgg
     3361 ctcatgccgc tttcggcctc gccagggcac cgatccactc ggccatgcgg ggccagagcc
     3421 gggcttcgtg ctcgctgcga aacggcccga agtggccgag gcgccgcacc ccgaggtcgc
     3481 cgggcgccac ccgccgcact tcggacggcg cgttctcgta aagatcgatc aggctgtggg
     3541 tgccgcgcag cgtcatcagt tcatcgtcgg tgatcgagag cgcctggacg ggaaacttcg
     3601 cgctggcata gcgttgacgc acagcctcgc cctcggcacc cacgctatag cggggattga
     3661 ggcaccactt gcgccactgc atgacgacgc ccgccggcag gtcgcccacc ttgcgcaggc
     3721 tgcggcccgg aaagtagccg cacagccacg tcgcgagcgg caccagcacg aaccagaaat
     3781 agaacacgat gcgcctgagc tgcggcgcat tctcgcgcca gtaaccgctg cccgccgcca
     3841 cgctgagcat gccgctgacc tttgcctggt tgttcaggag tcccggcaat tgcgcaccca
     3901 ggctgtggcc cagcaggtaa agcggccggt ccggcagtgc cgcatgcgcg tggtcgatgg
     3961 ccgcttcata gtcgcgagtc cagtcgaaca ggtcggcctt gaagccgcgc agggcgcggc
     4021 cgctgggtcc ggtctcgccg gagccgcggt agtcgaaggt caccacgcgc cagccctggc
     4081 ccgcgagcca ttgcgc
//
LOCUS       DASWIY010000322         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3526240, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000322 DASWIY010000000
VERSION     DASWIY010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3526240"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="VGA59_11585"
     CDS             complement(<1..1165)
                     /locus_tag="VGA59_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9720365.1"
                     /translation="MFSRFFIDRPIFAAVLSVFIVIAGLAAMRGLPIAQYPEIAPPVV
                     QVQAIYPGASAQVLEQTVAAPIENAINGVERMLYMSSTSAGNGVVQIQVTFEIGTDVN
                     QAALNVNNRVKQAEAKLPQEVRRQGVTVEKGSSSFLQVLAFYSPDGRYDDIFTSNYAT
                     LNVLDALKRVPGTTNVQIFGAKDYAMRIWLKPDRMAQFKLTTSDVAGAINEQNAQFAA
                     GKVGQSPIGGPQELVYTITTRGRMSEPREFEEIIVRSNPDGSAVRLRDVARVELGGKD
                     YDFIGRINGRPATLVGIFLQPGANALDVAGEVKNTMKTLAGRFPDGLTYSIPYDTTRF
                     VEVSIREVLITLAEAMVLVFLVVFVFLQNWRATLIPTIAVPVSLIGTFAGLHLL"
     gene            complement(1182..>2035)
                     /locus_tag="VGA59_11590"
     CDS             complement(1182..>2035)
                     /locus_tag="VGA59_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9720366.1"
                     /translation="LLARVKPLAEVGMVSKRDYDDSLSAEQVAVADLKAADARVNEAK
                     LNLGYTRVVAPVSGVAGGAAKSEGSLVSGPEVLLTTIVQTNPVEAVFGVPDAEHARIQ
                     ADIRAGRLMLPPGGFGVEVAAADGTLLARGGKLQFSEPLVNAATGTVRSQAELPNGDY
                     AIKPGQFVRVRLIGATRPDVVQVPQRAVLEGPQGKFVYVAAASDKPEMKGATVATSRP
                     VQAGEWVDLPAGKGWVIRGGLHAGDKVIVDGLMRIGPGAPVQVAEGPAVATLGAAAAA
                     PAQAASR"
BASE COUNT          317 a          721 c          665 g          332 t
ORIGIN      
        1 ccagcaggtg cagcccggcg aacgtgccga tcagcgagac cggcaccgcg atggtgggta
       61 tcagggtcgc gcgccagttc tgcaggaaga cgaacaccac caggaatacc agcaccatgg
      121 cttcggccag cgtgatgagc acttcgcgga tcgagacttc cacaaagcgc gtggtgtcgt
      181 acggaatgga gtacgtcagc ccgtcgggaa agcgcccggc cagcgtcttc atggtgttct
      241 tgacctcgcc ggcaacgtcc agcgcgttgg cgcccggctg caggaaaatg ccgaccagcg
      301 tggcgggccg gccgttgatg cggccgatga agtcgtagtc cttgccgccc agctccaccc
      361 gcgcgacatc gcgcaatcga accgcggacc catccgggtt cgagcgcacg atgatctcct
      421 cgaactcgcg cggctccgac atacggcccc gggtggtgat ggtgtagacc agctcctgcg
      481 gacctcctat gggcgactgg cccaccttgc ccgccgcgaa ctgggcgttc tgctcgttga
      541 tggcgccggc gacgtccgag gtggtgagct tgaactgtgc cattcggtcc ggcttgagcc
      601 agatgcgcat cgcatagtct ttcgcgccga agatctgcac gttggtcgtg cccggcacgc
      661 gcttcaacgc gtcgagcacg ttcagcgtgg cgtagttgct ggtgaagatg tcgtcgtagc
      721 ggccgtcggg cgaataaaag gccagcacct gcaggaagga ggacgagccc ttctccaccg
      781 tcacgccctg ccggcgcacc tcctgcggca gcttggcctc ggcctgcttc acgcgattgt
      841 tgacgttcag cgcggcctgg ttgacgtcgg tgccgatttc gaaagtcacc tggatctgca
      901 ccacgccgtt gccggccgag gtcgaactca tgtacagcat gcgctcgaca ccgttgatcg
      961 cgttttcgat cggggccgcc acggtctgct ccagcacctg cgcgctggcg ccgggataga
     1021 tcgcttgcac ctgcacgacc ggcggggcga tctccggata ctgcgcgatc ggcaggcccc
     1081 gcatggcggc caggccggcg atcacgatga agacggacag gaccgccgca aagatcgggc
     1141 ggtcgatgaa aaagcgggag aacatggcct ggccccccgg gttacctgga ggccgcttgc
     1201 gccggagccg ctgcggcggc ccccagcgtc gccacggccg gaccctccgc aacctgcaca
     1261 ggcgcgccgg ggccgatgcg catcaggcca tcgacgatca ccttgtcgcc ggcgtgcagt
     1321 cctccgcgga tcacccagcc cttgccggcc ggcaggtcca cccattcgcc ggcctgcacc
     1381 ggccgcgacg tggccacggt ggcccccttc atctccggct tgtcgctggc cgccgccaca
     1441 tagacgaact tgccttgcgg gccttcgagg actgcgcgct gcggcacctg caccacatcg
     1501 gggcgcgtcg ccccgatcag gcgcacccgc acgaactgtc ccggcttgat ggcgtagtcg
     1561 ccgttgggca gttcggcttg ggagcgcacc gtgcccgtcg ccgcgttgac cagcggttcg
     1621 ctgaactgga gcttgccgcc gcgcgccagc agcgtcccgt cggccgcggc cacttcgacg
     1681 ccgaaccccc ccggcggcag catcagccgc ccggcgcgga tatcggcctg gatgcgcgca
     1741 tgctccgcat ccggtacgcc gaacacggcc tcgacggggt tggtctgcac gatagtggtg
     1801 agcaggactt ccggtccgga caccaggctg ccctcggact tggccgctcc gccggcgaca
     1861 ccggacaccg gcgccaccac cctcgtgtag cccaggttga gcttcgcctc gttcaccctt
     1921 gcgtcggccg ccttgagatc ggccaccgcg acctgctcgg cggacaggga atcgtcgtag
     1981 tcgcgcttgc tcaccatgcc aacctcggcc agcggcttga cgcgcgccag caggc
//
LOCUS       DASWIY010000323         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3188519, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000323 DASWIY010000000
VERSION     DASWIY010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3188519"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..860
                     /gene="xth"
                     /locus_tag="VGA59_11595"
     CDS             84..860
                     /gene="xth"
                     /locus_tag="VGA59_11595"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX9720367.1"
                     /translation="MKITTWNVNSLTARLQHVLEWTAANPVDVLCLQELKLTDDKFPL
                     EVLRSAGYEHCAVFGQKTYNGVAIMSRHPLRDVVRNIVGFDDEHSRVIAATLDAPGGA
                     LRLVNGYFVNGQAPGTAKFEYKMSWLRGLRDYLRDEVARHRRLALLGDFNIAPEDRDS
                     WDPEGLRETIHHTSDERAHFRKLVELGLCDSFRLFDQPEKSFSWWDYRMLGYQKNRGL
                     RIDHILVSEALKPEVKACTIDRAPRKWKQPSDHAPVTVEL"
     gene            complement(1063..2604)
                     /gene="ntrC"
                     /locus_tag="VGA59_11600"
     CDS             complement(1063..2604)
                     /gene="ntrC"
                     /locus_tag="VGA59_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEX9720368.1"
                     /translation="MKPIWIVDDDQSIRFVLEKALLREDLPTRSFTSPREVLQALDSG
                     DDENGPQILVSDIRMPGGSGLDLLAKVKEKLPGLPVIIMTAFSDLDSAVSAFQGGAFE
                     YLPKPFDLPKAIELIRRAVEESQREEVAEERMADTPEMLGQAPAMQDVFRAIGRLSQS
                     NVTVMITGESGTGKELVARALHKHSPRASGPFVAINTAAIPKDLLESELFGHERGAFT
                     GAQTMRRGRFEQAEGGTLFLDEIGDMPFDLQTRLLRVLSDGHFYRVGGHNAVKANVRV
                     IAATHQDLEQRVKEGAFREDLFHRLNVIRLRLPALRERLDDIPMLTRHFLQQSAKQLG
                     VEPKRISDAALARLSTFAFPGNVRQLENICHWLTVMAPAQLIEPKDLPPEVAPALGET
                     VISVSAPPASEPPRAAARDDDSRVDAPAAASGALLAAAGTTAVSDSRGADPRSWELGL
                     EAEALELLASGRHDVWDALTKRFESKLILTALANTRGRRIEAAQKLGIGRNTITRKIQ
                     ELGLE"
BASE COUNT          483 a          921 c          878 g          470 t
ORIGIN      
        1 cgttgcccta gaatcggccg ctggcgtatt agcaatgctt cacatctgat ggcactatcg
       61 cccgcccaag tgcggcacgc aaaatgaaga tcaccacctg gaacgtcaat tcgctgacgg
      121 cgcgcctgca gcatgtcctg gaatggaccg ccgccaaccc cgtcgatgtg ctgtgcctgc
      181 aggagctgaa gctgaccgac gacaagttcc cgctcgaggt cctgcggtcg gcgggctacg
      241 agcactgtgc cgtgttcggc cagaagacgt acaacggcgt ggccatcatg agccggcacc
      301 cgttacgcga cgtcgtgagg aacatcgtcg gcttcgacga cgaacactca cgcgtgatcg
      361 cggcgacgct cgacgccccc gggggcgcac tgcgactggt caacggctac ttcgtgaacg
      421 gccaggcgcc gggcacggcc aagttcgaat acaagatgag ctggctgcgc gggctgcgcg
      481 actacctgcg cgacgaagtg gcccggcatc ggcggctggc actgctgggc gacttcaaca
      541 tcgcacccga agaccgcgac tcctgggatc ccgagggcct gcgcgagacg atccaccaca
      601 ccagcgacga gcgcgcccat ttccgcaagc tggtggagct gggactttgc gacagcttcc
      661 gcctgttcga ccagccggag aaaagcttca gctggtggga ctaccgcatg ctgggctacc
      721 agaagaaccg cgggctgcgc atcgaccaca tcctggtgag cgaggcgctg aagccggagg
      781 tcaaggcgtg cacgatcgac cgcgcgcccc gcaagtggaa gcagcccagc gaccatgcgc
      841 cggtgacggt ggaactctga ggttgtcatt gcgggcctga tccgcaatcc acgacggcgc
      901 tcgaaacatg gatcccgggt caagcccggg atgacaaaca gctcatcatg gaccccggtt
      961 caggcccggg atgacaaacc ctctgtcatt gcgggcctga cccgcaatcc gcgacggcgc
     1021 tcgaaccatg gatcccgggt caagcccggg atgacagctt acctactcca gccccaactc
     1081 ctggatcttg cgcgtgatcg tattgcgccc gatgccgagc ttttgcgcgg cttcgatgcg
     1141 ccgcccgcgg gtgttcgcca gcgcggtgag gatcagcttg gactcgaatc gcttcgtgag
     1201 tgcgtcccag acatcgtgcc gcccgctcgc cagcaattcc agcgcttcgg cttcgagccc
     1261 caactcccag ctgcgcgggt ccgcgccacg cgaatccgat acggccgtgg taccggctgc
     1321 ggcaaggagc gcgccggacg cggccgcggg cgcatccacg cgcgaatcgt cgtcccgcgc
     1381 ggccgcccgc ggcggctccg aggctggagg cgccgagacg ctgatgaccg tttcacccag
     1441 cgccggcgcc acttcaggcg gcaggtcctt gggctcgatc agctgggccg gcgccatgac
     1501 ggtgagccag tggcagatgt tctccagctg acgcacattg cccgggaagg cgaacgtact
     1561 cagccgggcc agcgccgcat ccgagatccg cttcggttcc acgcccagct gcttggcgct
     1621 ttgctgcagg aaatggcgcg tgagcatcgg gatgtcgtcc agccgctcgc gcagcgcggg
     1681 cagccgcagg cggatcacat tgagccggtg gaacaagtct tcgcggaaag cgccctcctt
     1741 gacgcgctgc tccagatcct ggtgggtagc cgcgatcaca cgcacattcg ccttgacggc
     1801 gttgtggccg cccacccggt agaaatggcc gtccgaaaga acacgcagca ggcgcgtctg
     1861 caggtcgaaa ggcatgtcgc cgatttcatc caggaacagg gttccgccct cggcctgctc
     1921 gaagcgcccg cgccgcatcg tctgggcgcc ggtgaaggcg ccccgctcgt gcccgaacag
     1981 ttcgctttcc agcaggtcct tggggatggc cgccgtgttg atggccacga acgggccgct
     2041 ggcccggggg gagtgcttgt gcagggcgcg cgcgaccagt tccttgccgg tgcccgactc
     2101 gccggtgatc atgaccgtca cgttgctctg cgacaggcgg ccgatggcgc ggaacacgtc
     2161 ctgcatcgcg ggtgcctgac ccagcatttc gggcgtgtcg gccatgcgtt cctcggccac
     2221 ttcctcgcgc tggctttctt ccacagcgcg gcggatcaac tcgatcgcct tgggcagatc
     2281 gaatggtttg ggcaagtatt cgaacgcgcc tccctggaac gccgacacgg cgctgtccag
     2341 gtcggagaat gccgtcatga tgatgacggg aagccccggc agcttctcct tgaccttggc
     2401 caggaggtcc aggcccgatc cgcccggcat ccggatatcg ctcaccagga tctgggggcc
     2461 gttctcgtcg tcgcccgagt ccagggcctg cagcacctcc cgcgggctgg tgaagctgcg
     2521 ggtgggcaga tcctcgcgca acagcgcctt ttccagcacg aagcggatgg attggtcatc
     2581 gtcaacgatc cagatcggct tcatgttgtc aacctcggtc ccttgtaagt gtcattgcgg
     2641 gctcgacccg caatccatgg atcccgggtc aagcccggga tgacaaaacc aatctttatg
     2701 gcaacggaat caatatcttg aaatccgtcc tcccaggcac cgtgtcgcac tc
//
LOCUS       DASWIY010000324         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2454226, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000324 DASWIY010000000
VERSION     DASWIY010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2454226"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VGA59_11605"
     CDS             complement(<1..628)
                     /locus_tag="VGA59_11605"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX9720369.1"
                     /translation="MEPQWLRRLREILWGERVALTRYQHELRQAGRYIYALARDLLDG
                     QINMRAMSLIYTTLLSLVPLLALGFSLLKGLGVHNSLQPVLDRFLAPLGPQGMQLSQN
                     IITFVENIKVGVLGSFSVMLLLYAVLSMIQKVEDSFNFIWRIEARRKLGQRVSEYLSV
                     LLVGPAAIVLALGTTATVANSEFVARLAQVEPLGLVFYVVGRLVPYAVI"
     gene            813..1256
                     /locus_tag="VGA59_11610"
     CDS             813..1256
                     /locus_tag="VGA59_11610"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX9720370.1"
                     /translation="MTFEKAVKEVQGRLETVGERSQDVAKVSLKTLTKANEIVISGVQ
                     DIVTAQTAAAKDLFSASKQSFDKAVAAGLVAVVKSPVEYIPDGKDTIVGAFNETVDTV
                     AKTGEKLVKVVKTGYQDVAKTIQGKPLRKATRHAKKATRSVQAAA"
     gene            1332..>1585
                     /locus_tag="VGA59_11615"
     CDS             1332..>1585
                     /locus_tag="VGA59_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720371.1"
                     /translation="MIDRTLVQSRRHRRRGEAYAHRAVGGLALIVMHELDALSGHVAE
                     VRERLAVARKARGLGELVRDQVDLLPETRVRLTLDQRERVA"
BASE COUNT          301 a          512 c          533 g          239 t
ORIGIN      
        1 cgatcacggc gtacggcacc agccgtccga ccacgtagaa gaccaggccc agcggctcga
       61 cctgcgcgag tctcgcgacg aactcgctgt tggccacggt cgcggtggtg cccagcgcga
      121 gcacgatcgc cgcgggcccg accagcagca ccgacaggta ctcgctgacg cgctgcccga
      181 gcttgcgccg cgcctcgatg cgccagatga aattgaagct gtcctcgacc ttctggatca
      241 tcgacaggac ggcatagagc agcagcatca cgctgaagga gccgagcacg ccgaccttga
      301 tgttctcgac gaaggtgatg atgttctggg acagctgcat gccctgcggc cccagcggcg
      361 cgaggaagcg gtcgagcacc ggctgcaggc tgttgtgcac gcccaggccc ttgagcagcg
      421 agaagcccag cgccagcagc ggcaccagcg acagcagcgt ggtgtagatc aggctcatcg
      481 cccgcatgtt gatctggccg tcgagcaggt cgcgcgccag ggcgtagatg tagcgcccgg
      541 cctggcgcag ctcgtgctgg tagcgggtca gggccacgcg ctccccccac aggatttcgc
      601 gcagccggcg cagccattgc ggttccacgg tttcggggtt ttcgggaggg ggcgggcccg
      661 taggataccc cccaaagcca gccgttcgtc ggcccgatct ggggccggct tgacatgttg
      721 cactgcagca attacattgc actgcacaaa ttgctgcggt gcagcagaac tagaaaacaa
      781 gcggtccccg tcaactactt atggagattt acatgacatt cgaaaaggct gtgaaggaag
      841 ttcagggccg tcttgagacg gtgggcgagc ggtcgcagga cgtcgccaag gtttcgctga
      901 agacgctgac caaggccaac gagatcgtga tctcgggcgt gcaggacatc gtgaccgcgc
      961 agaccgcggc cgcgaaggac ctcttcagcg ccagcaagca gagcttcgac aaggcagttg
     1021 ccgccggcct ggtggccgtc gtcaagagcc cggtcgagta catcccggat ggcaaagaca
     1081 cgatcgtcgg cgcgttcaac gagacggtcg ataccgtcgc gaagaccggc gagaagctcg
     1141 tgaaggtcgt gaagaccggc taccaggacg tcgccaagac catccagggc aagccgctgc
     1201 gcaaggccac gcgccatgcg aagaaggcca cccgctcggt tcaggcggcc gcctaagtcc
     1261 cagcgtcaat gcggtacgca agggggctgg aattccagcc ccctttttta ttggagccag
     1321 ggagatcccc catgattgac cgaaccctcg tccagtcgcg gcgtcaccgc cggcgcggcg
     1381 aggcctatgc ccaccgggcg gtgggcggcc tggccttgat cgtgatgcac gagctcgacg
     1441 cgctgtcggg ccacgtcgcc gaagtgcgcg agcgcctggc ggtcgcgcgc aaggcacgcg
     1501 gcctgggcga gctcgtgcgc gaccaggtcg acctgctgcc ggaaacgcgc gtgcgcctca
     1561 cgctcgacca gcgcgagcgg gtcgc
//
LOCUS       DASWIY010000325        13088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3189456, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000325 DASWIY010000000
VERSION     DASWIY010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 13088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13088
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3189456"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VGA59_11620"
     CDS             complement(<1..460)
                     /locus_tag="VGA59_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9720372.1"
                     /translation="MESKTIRFIRRGEIVSLSNVPPERTLLELLREDLGATGTKEGCA
                     EGDCGACTVVLGELAPGPAGQGLRYRAINSCIRLAHSVDGMALWTTEDLAGGDGTLHP
                     AQEAMVRSHGSQCGFCTPGFVMSLFAMYQNHVCRGEAITRELAREELSGNL"
     gene            634..2274
                     /locus_tag="VGA59_11625"
     CDS             634..2274
                     /locus_tag="VGA59_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720373.1"
                     /translation="MSIPRLQLAHITKRYPAVVANNDVSLTVQPGEIHAVLGENGAGK
                     STLMKIIYGSVKPDEGSMLWNGQPVHIRNPQEARALGISMVFQHFSLFDTLTVAENVW
                     LGLSRSLTLNQVAHSIAAKAGEYGLDIDPARPVHTLSVGEMQRVEIIRALLTNPKLLI
                     LDEPTSVLTPQAVEKLFVVLKKLGSEGCSILYISHKLHEIRELCTACTVLRAGEVTGV
                     CNPQYESNASLSRLMIGAEPPQLEHHERKPGEVVLKVTDLHLAREDQFGVDLDGVSLE
                     VRAGEVVGIAGVSGNGQLELLHALSGEDTRAQPGEIQVFGKKVGRFGPAHRRHLGVHF
                     VPEERLGRGAVPTLGLAHNLMLTRTEAIHQSGWIRVKALEKQARAIIARFNIKAGGPH
                     AAARSLSGGNLQKFIVGREIDANPKLLIVSQPTWGVDVGASAQIRGEILALRDAGCAV
                     LVVSEELEELFEISDRLHVIAKGRLSPSIDRARATVSLIGEWMSGLWGVQPLRQPASP
                     ALTPTLSRKRERESDTFSSVEGRGEGPSGSSQSGSNNA"
     gene            2267..3346
                     /locus_tag="VGA59_11630"
     CDS             2267..3346
                     /locus_tag="VGA59_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9720374.1"
                     /translation="MLKLELRPQPSRFWSLASPILALAITVVIGVLLFVALGKDPVRG
                     LQVFFWEPIRSRYALGELMVKATPLLVIALGLAVCFRSNVWNIGAEGQFIIGAVVAGG
                     VALLADKDSSRWIVLAIVLAGTLGGMAWAGITAVLRDKFNANEILVSLMLVYVADMVL
                     SYLVYGPWKDPAGYNFPQTKTFEAATQIPRLMQGSRVSIGLVIALAGVAGLWVFLFRT
                     RAGFAQQVGGLAPAAARYAGFSSRRALWIALLASGGAAGLAGALEVAGPIGQLTPYVP
                     AGYGFAAIIVAFVGRLHPVGMVFSAILMSMFYIGGELAQSRLGLPKSLTGVFQGLLLF
                     TLLACDTLMTYRIRFTPLSGLKGAR"
     gene            3346..4266
                     /locus_tag="VGA59_11635"
     CDS             3346..4266
                     /locus_tag="VGA59_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9720375.1"
                     /translation="MESYSLLLAATLNAGTVLAIAALGLLINEKAGIVNLGAEGMMLC
                     AAIAGFAAVVHTGSDWLGFAAGMGAGALLAAIFGLLVIWLNTNQYATGLALSLFGAGF
                     SAFAGITYVQQVLPERRAFSVPLLADIPLVGPALFRQHPMVYVTIALVAWLIWFLYRS
                     RAGLVLRSVGQSPESAHALGYPVRRIRLAAVVAGGALCGLAGAYISIIYTPLWVEGMV
                     AGKGWIALALTTFATWRPARVLLGAYLFGGVTMLQFHFQGIGVEVPSQFLTMLPYLAT
                     IVVLALISRNPAWIRINMPASLGKPFYPGS"
     gene            4307..5467
                     /locus_tag="VGA59_11640"
     CDS             4307..5467
                     /locus_tag="VGA59_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9720376.1"
                     /translation="MTDLKKRSMFKLAALSALAATALVGCGKKEEPPPPAPAPAPVAA
                     PAPKPEPLKIAFAYIGPVGDGGWTFAHDNGRKAMEKELGDKVATSFVENVPESADAER
                     VIRDMVGQGNKLVFGTTFGYMEPMLKVAADHKDVKFEHATGYKTAENLRTYDSRTYEG
                     AYMAGVIAGAMTKTNTLGVVGSVPIPEVIRNINSFTLGAQSVNPKIKTKVVWVNKWFD
                     PPKESEAATSLINGGADVLMQNTDSPAVLKTAQDKGKRAFGWDSDMTAYGPKAHLGSA
                     VINWGPYYIKATKDALEGNWTTGQAWWGVKEGAIDFVSVAEDVPADIKKKVEDVKAGL
                     KDGSFSIWKGPIADNTGKQVLAKDAVADDKFLGGINWYVKGVEGKVPGGEKK"
     gene            5742..6116
                     /locus_tag="VGA59_11645"
     CDS             5742..6116
                     /locus_tag="VGA59_11645"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEX9720377.1"
                     /translation="MKLLSVLLSLALTAPAVQGQTSPAQAKPAAPAPARQLPLARGEV
                     LEVNRQEASVLVKHGPIPSLGMDPMTMVFLVPDAKLLALLKPGDKIRFAAAWKKGDYL
                     ITRAEVQKPGAVQRPMSAPPGK"
     gene            6181..7221
                     /locus_tag="VGA59_11650"
     CDS             6181..7221
                     /locus_tag="VGA59_11650"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX9720378.1"
                     /translation="MPSTIPVAPERLPDLLRRMPKAELHIHIEGSLEPELIFALSERN
                     KVRIPYSSVEELRRAYAFTNLQSFLDIYYAGASVLREEQDFFDMAWAYLQRAAADNVV
                     HTEMFFDPQTHTARGVSMETVITGLHRACTQAGPALGVSASLIMCFLRHLSEQEAFET
                     LEQALPFQDKFIGVGLDSSEVGHPPEKFAQVFARCGELGLHRVAHAGEEGPPAYVWTA
                     LDLLKVERIDHGVQSSKDAALMARLAKDRIPLTVCPLSNLKLCVFPKLADHNIGKLLD
                     AGLVATVNSDDPAYFGGYMNDNFVQTFAATGLTATHAYQLAKNSFDASFIHESMKRRY
                     TDLLDATFAAFE"
     gene            7328..8416
                     /locus_tag="VGA59_11655"
     CDS             7328..8416
                     /locus_tag="VGA59_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9720379.1"
                     /translation="MYKNLAAALAAACFFSPAFSQSKSQPKPQEPLKVGFVYVAPITE
                     AGWVQQHERGRQAVEVAFAGKVKTSFVENVAEGADSERVIRDLAKQGHKLIFTPSFGY
                     MEPTLKVAKEFPDVKFESITGYKTAPNVAVANARYYEGRYLAGIAAGRMTKTNVAGYV
                     AGFPIPEVLQGINAFTLGMRSVNPKAQVKVVWLNAWFDPPRERDAAMTLFNQDVDVIA
                     FHTASTAVMAAAQERGKMAVAYHSDMRKAAPDAQIVAVTHHWGDYYVRRVQALLEGRW
                     QTGSFWGGVKHAMVRAGDFGPKVPKAVRDEVLVRQGEIAKGKLLPFSGPIVDNEGKQV
                     LAKGKAMTDEQILSMNFLVAGVQGKVAK"
     gene            8421..9719
                     /gene="guaD"
                     /locus_tag="VGA59_11660"
     CDS             8421..9719
                     /gene="guaD"
                     /locus_tag="VGA59_11660"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HEX9720380.1"
                     /translation="MQAFRSAILRFADDQSAVYEQDGLLVIGPDARGRQVVHAVGDYH
                     SLAPAYPGLAVEHLPGRIIAPGFIDLHIHFPQTDLIGSPAAGLLPWLEQYTFPHESRF
                     SENAHAQELAIFFLDELLRHGVTTALAFASSHPPSVNALMAEAQRRGLRLIAGKVLQD
                     RHSPDGVRDETEQSLVDSEDLIRKWHGVDRLGYAITPRYAPSCSEAQLLGAGELAARY
                     PEVWIQSHVAENLEEVKWARELFPASRSYLAIYDDFGLMRERAIYAHCIHFDDEDRAM
                     MRATGAAAAVSPTSNLFLGSGFFDYEGAQRAGFKYGLASDVGGGTSFSPFHTMLAAYY
                     VGREGRSKPGLSLSPQHLWWQHTAGAAQALGLQGVIGNLQPGCEADFIVLDPGATPLL
                     ARRTASAGTLAELLFALIVLGDDRVIQEVSISKNTEEVRS"
     gene            complement(9879..10451)
                     /locus_tag="VGA59_11665"
     CDS             complement(9879..10451)
                     /locus_tag="VGA59_11665"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HEX9720381.1"
                     /translation="MSDDTPRLPLPRALIGWQSMFMGVALLASARSKDHRTRNGACIV
                     TPDNRIIGVGYNGLPRGCDDADPVYWSDNDDDAFRSRHSYVVHAEINAILNCVVLPLR
                     NATIYTTQFPCPRCAQAIIQAGIRSVIYLREKTHHVDSNAASSKMFAASGVACEPLES
                     IDNDAHAWSTQFGTFIVGRDSRTIFNDEDV"
     gene            complement(10713..11627)
                     /locus_tag="VGA59_11670"
     CDS             complement(10713..11627)
                     /locus_tag="VGA59_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta(1)-pyrroline-2-carboxylate reductase
                     family protein"
                     /protein_id="HEX9720382.1"
                     /translation="MTRLLEPTQTAACLAFPALVAQLRELLADASVRVPERLVQPLPG
                     GGSLFVMPALDRRIAIAKLITFTPANVGTRRPAIQGDVVVFDVATGERTLILDGPTVT
                     ARRTAAVSLLAAQCLAPRKEGPLLIVGAGAQGRAHLEAFAQGLPLRQVFIASRSEASA
                     QALAGYARALGLHAGTVVDANQAVADCPLIVTCTPANDVVLHALPREDAFIAAVGAFT
                     PSMVELDSGLCRHCATHGRVVVDTPQAVHEAGDLLQAGLDVSGFPTLREVVLGTAPAS
                     TRAPILFKSCGWAGWDLAAARVALALQK"
     gene            11747..12091
                     /locus_tag="VGA59_11675"
     CDS             11747..12091
                     /locus_tag="VGA59_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720383.1"
                     /translation="MLSRLPERPAASRDRLFRAVLVALAIGQLVAFWMLCSHQVRKAQ
                     MRDVGLAVQRVAIADCLQYIPRATLNSCINRVDPSRESSAVMATGHKPTAAPDATRAT
                     MSSGTPVSVSYQ"
     gene            12235..12555
                     /locus_tag="VGA59_11680"
     CDS             12235..12555
                     /locus_tag="VGA59_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720384.1"
                     /translation="MNNTVAHSGFPWSDPMLKSLDASDDFDAMPDATRYCPNQLLSAF
                     CLLMAGHGRSISTAMMLGDREYAMWQLARAHTLDDRELRSVAARLFGYFDDVHAPVPA
                     GLHD"
     gene            12678..>13088
                     /locus_tag="VGA59_11685"
     CDS             12678..>13088
                     /locus_tag="VGA59_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9720385.1"
                     /translation="MTPTLPSWETLDTGDTLPPLELPPITRLALALYCGASGDHNPIH
                     VDTDFAKSAGMPDVFAHGMLSAAWLGRLLTNWVPQSAIRSLDVRFAVITHVGERITCT
                     GKVTEKFEDQGRRYARLALTTTNQDGLVKLSGEAL"
BASE COUNT         2309 a         4300 c         4214 g         2265 t
ORIGIN      
        1 acaggttgcc ggagagctct tctcgcgcca gctcgcgcgt gatcgcttcg ccgcggcaga
       61 catggttctg gtacatggcg aacaggctca tcacgaagcc gggcgtgcag aagccgcatt
      121 gcgagccgtg gctgcgcacc atcgcctcct gcgccgggtg cagcgtgccg tcgccgccgg
      181 ccaggtcctc cgtggtccac agcgccatgc cgtcgacgga atgggccagc cggatgcagc
      241 tgttgatggc gcgatagcgc aggccttggc cggccgggcc cggtgccaat tcgcccagca
      301 cgacggtgca ggcgccgcag tcgccctcgg cgcagccctc tttggtgccg gtggcgccca
      361 ggtcctcgcg cagcagttcc agcagcgtac gctcgggtgg tacattgctc agcgaaacaa
      421 tctcgccccg gcggatgaat ctgatcgttt tcgattccat gaagctcagt atggttgatt
      481 tacagcgtcc cttgtaagtg tggcgttgtc attgcgggcc atgacgctcc ggcccggaac
      541 cgcaatccat ggatcccgga tcaagtcctg acagccggga tgacagccgg gatgacagaa
      601 tactcggcaa gccagtactg agacccagcc caagtgagca tcccaagact ccagctcgcc
      661 cacatcacca agcgctaccc ggccgtggta gccaacaacg atgtgtcgct caccgtccag
      721 cccggcgaaa tccatgccgt gctgggggag aacggtgccg gcaagtcgac gctgatgaag
      781 atcatctacg ggtccgtgaa gcccgacgag ggcagcatgc tgtggaacgg ccagccggtg
      841 cacatccgca acccgcagga agcgcgtgcc ctgggcatca gcatggtgtt ccagcacttc
      901 agcctgttcg atacgctcac ggtggccgag aacgtgtggc tgggcctgtc caggagcctg
      961 acgctgaacc aggtggcgca cagcatcgcg gccaaggcgg gtgaatacgg gctggacatc
     1021 gacccggcgc ggccggtgca cacgctttcc gtgggcgaga tgcagcgggt cgagatcatc
     1081 cgcgccctgc tcaccaatcc caagttgctg atcctggacg agccgacctc ggtgctgacg
     1141 ccgcaggcgg tggaaaaact gttcgtcgtg ctcaagaagc tgggctccga gggctgcagc
     1201 atcctctaca tcagccacaa gctgcacgag atccgcgagc tgtgcaccgc ctgcaccgtg
     1261 ctgcgcgcgg gcgaggtcac aggcgtgtgc aacccgcagt acgagtcgaa cgcatcgctg
     1321 tcacggctga tgattggcgc cgagccgccg cagctcgagc accacgagcg caaacccggc
     1381 gaggtggtgc tgaaggtgac ggatctgcac ctcgcgcgcg aagaccagtt cggcgtcgac
     1441 ctggatggcg tctcgctgga agtgcgcgcg ggcgaagtcg tgggcatcgc cggcgtgtcg
     1501 ggcaatggcc agctggaact gcttcatgcc ctgtcgggcg aggacacccg ggcgcagccg
     1561 ggcgagatcc aggtattcgg aaaaaaggtc ggccgcttcg ggccggcgca ccgccgccac
     1621 ctgggcgtgc acttcgtgcc cgaagaacgg ctggggcgcg gtgccgtgcc cacgctgggc
     1681 ctggcccaca acctcatgct cacgcgcacg gaagccatcc accagagcgg ctggatccgc
     1741 gtcaaggcgc tggagaagca ggcgcgggcc atcatcgcgc gcttcaacat caaggctggt
     1801 ggcccgcacg cggccgcccg ctcgctgtcg ggcggcaacc tgcagaaatt catcgtcggg
     1861 cgcgagatcg atgccaaccc caagttgctg atcgtgtccc agccgacctg gggcgtggac
     1921 gtgggagcct ccgcgcagat ccgcggtgaa atcctggcgc tgcgcgacgc cggctgcgcc
     1981 gtgctggtcg tcagcgagga gctggaagag ctgttcgaaa tcagcgaccg cctgcatgtg
     2041 atcgccaagg gccgcctgtc gccgtccatc gaccgcgcgc gcgccaccgt ctcgctcatc
     2101 ggagaatgga tgagcgggct gtggggggtg cagccgctgc ggcagcctgc ctcgccagcc
     2161 ctcaccccaa ccctctcccg caagcgggag agggagtcag acaccttctc ctccgtcgag
     2221 ggcaggggtg aggggccttc cggttccagc caatcgggat caaacaatgc ttaagctcga
     2281 actgcgcccg cagccctcgc ggttctggag cctggcttcg ccgatcctgg cactggccat
     2341 caccgtggtc atcggtgtgc tgctgttcgt cgcactgggc aaggacccgg tcagggggct
     2401 gcaggtcttc ttctgggagc cgatcaggtc gcgctatgcg ctgggcgaac tcatggtcaa
     2461 agcgacgccg ctgctggtca tcgcactggg actggcggtg tgcttccgct ccaacgtctg
     2521 gaacatcggc gccgagggcc agttcatcat cggcgcggtg gtggccggcg gtgtcgcctt
     2581 gctggccgac aaggactcga gccgctggat cgtcctggcc atcgtgctgg cgggcacgct
     2641 cggcggcatg gcctgggccg gcattacggc ggtgctgcgc gacaagttca acgccaatga
     2701 aatcctggtg agcctgatgc tggtctatgt ggccgacatg gtgctcagct acctcgtcta
     2761 tggcccctgg aaggacccgg ccggctacaa cttcccgcag accaagacct tcgaggccgc
     2821 cacgcagatc ccgcgcctga tgcagggttc gcgcgtcagc atcggcctcg tgatcgcgct
     2881 ggccggcgtg gcggggctgt gggttttcct gttccgcacc cgcgccggct ttgcgcagca
     2941 ggtggggggg ctggcgccgg ccgccgcgcg ctacgcgggc ttttcctcgc gccgggcgct
     3001 gtggatcgcg ctactcgcct cgggcggcgc ggcggggctg gcgggcgcac tggaggtggc
     3061 gggaccgatc ggccagctca caccctatgt gccggcgggc tacggattcg cggccatcat
     3121 cgtcgccttc gtcgggcggc tgcatccagt gggcatggtc ttctccgcga tcctgatgag
     3181 catgttctac atcggcggcg agctggcgca gtcgcgcctt ggattgccca agtcgctgac
     3241 cggcgtattc cagggcctgc tcctgttcac gctgctggcg tgcgacacgt tgatgactta
     3301 ccggatccgt ttcacgccgc tgtcaggcct caagggagcg cgttgatgga gtcctattcg
     3361 ctccttctcg cggccacgct caacgctggc accgtgctgg ccatcgcggc cctcggcctg
     3421 ttgatcaacg aaaaggccgg catcgtcaac ctgggcgccg agggcatgat gctgtgcgct
     3481 gcgatcgccg gcttcgccgc tgtggtccac actggcagcg actggctggg cttcgcggcc
     3541 ggcatgggcg cgggcgcgct gctggcggcg atcttcgggc tgctcgtgat ctggctcaac
     3601 accaaccagt acgcgacggg actggcgctc agccttttcg gtgccggctt ttccgcattc
     3661 gccggcatca cctacgtgca gcaggtcctg cccgagcgac gtgctttttc cgtgccgctg
     3721 ctggcggaca ttccgctggt cggccctgcg ctgttccgcc agcatcccat ggtctacgtg
     3781 accatcgccc tcgtcgcgtg gctcatctgg ttcctgtacc gctcgcgcgc gggcctggtg
     3841 ctgcgctcgg taggccaatc gcccgaatcg gcgcacgccc tgggctatcc ggtgcggcga
     3901 atccggctcg cggcggtggt ggccggcggc gcgctctgcg ggctggcggg cgcttacatc
     3961 tcgatcatct acacgccgct gtgggtcgag ggcatggtgg ccggaaaagg ctggatcgcc
     4021 ctcgcgttga ccacgttcgc cacctggcgc ccggcgcgcg tgttgcttgg cgcctacctg
     4081 ttcggcgggg tgacgatgct gcaattccat ttccagggca tcggcgtcga ggtgcccagc
     4141 cagttcctca ccatgctgcc ctatctcgcg acgatcgtgg tgctggcgct gatctcccgc
     4201 aatccggcat ggatcaggat caacatgccg gcctcgcttg gtaaaccgtt ttatcccggc
     4261 tcataattgc tgctttgcca aaaccatccc tgcagaagga actgacatga ccgacctgaa
     4321 gaagcgctcg atgttcaagc tggccgcgct ttccgcgctt gcggcgacag ccctggtggg
     4381 ctgcggcaag aaggaggagc cgccgccgcc cgcgcccgca ccggcccccg tggcggcccc
     4441 ggcccccaag cccgagccgc tcaagatcgc ctttgcctac atcggaccgg tgggcgacgg
     4501 cggctggact ttcgcgcacg acaacgggcg caaggcgatg gagaaggaac tgggcgacaa
     4561 ggtcgccacc agcttcgtcg agaacgtgcc cgaatcggcc gacgccgagc gcgtgatccg
     4621 cgacatggtg ggacagggca acaagctcgt tttcggcacg accttcggct acatggagcc
     4681 catgctcaag gttgccgccg accacaagga cgtgaagttc gagcacgcca ccggctacaa
     4741 gaccgccgag aacctgcgca cctacgacag ccgcacttat gaaggcgcgt acatggccgg
     4801 cgtgatcgcc ggcgccatga ccaagaccaa caccctcggc gtggtgggct cggtgccgat
     4861 ccccgaggtg atccgcaaca tcaacagctt cacgctgggc gcgcagtcgg tcaacccgaa
     4921 gatcaagacc aaggtggtgt gggtcaacaa gtggttcgat cccccgaagg aatccgaagc
     4981 ggccacgtcg ctgatcaacg gcggcgccga cgtgctgatg cagaacaccg attcacccgc
     5041 cgtgctgaag accgcgcagg acaagggcaa gcgcgcattc ggctgggaca gcgacatgac
     5101 ggcgtacggc cccaaggctc acctcggctc ggctgtgatc aactgggggc cttactacat
     5161 caaggccacg aaggacgcgc tggaaggcaa ttggacgacc ggccaggcct ggtggggcgt
     5221 caaggaaggc gctatcgact tcgtgtcggt cgccgaggac gtgccggccg acatcaagaa
     5281 gaaggtcgag gacgtcaagg ccggcctgaa ggacggcagc ttctcgatct ggaagggccc
     5341 catcgccgac aacaccggca agcaggtgct ggcgaaggac gccgtggccg acgacaagtt
     5401 cctgggcggc atcaactggt acgtcaaggg ggtggaaggc aaggtgccgg gcggcgagaa
     5461 gaaataagga tccgtcattg cggggtgagc ccgtccctgg actcgatccg ggaacccgca
     5521 atgcatggat cccgggtcga gcccgggatg acagcctttg aatgctgtca ttgctgtcaa
     5581 tgctgtcaat gctgtcaatg ctgtcaatgc tgtcattgct gtcattgctg tcattgctgt
     5641 cattgcgggc ttgacccgca atccatgcct cggttcccat gatcgaagag ctgctccccg
     5701 gcagctcttt ttttgcccac agcaactaca ctgcccattc catgaagctc ctttccgtgc
     5761 tcctgtcatt ggcattgacc gctccggctg tgcagggcca gacctcgccc gctcaagcga
     5821 agcccgctgc acccgcgccc gcccggcaac tgccgttggc gcgcggcgag gttctcgaag
     5881 tcaacaggca ggaagcgtcg gtcctggtga agcacggccc cattcccagc ctgggcatgg
     5941 atccgatgac gatggtattc ctggtacccg acgccaaact tcttgcgctg ctcaagccgg
     6001 gcgacaagat ccgcttcgcc gcggcctgga agaagggtga ctacctgatc acgcgcgccg
     6061 aggtacagaa gcctggcgcc gtgcagcgcc cgatgtctgc gccgccgggc aagtgagccc
     6121 ttgtgcaaca gcgcgaatgc ggttgaaaat cgggcccacg aatcaacccg ctttcctcct
     6181 atgccttcca ccatccccgt cgcgcccgaa cgcctgcccg atctgctgcg ccgcatgccc
     6241 aaggccgagc tgcacatcca catcgagggc tcgctcgagc ccgagctgat cttcgcgctg
     6301 tccgagcgca acaaggtgcg gattccctat tccagcgtcg aagagctgcg ccgggcctac
     6361 gccttcacca acctgcaaag cttcctggac atctactatg cgggcgccag cgtcctgcgc
     6421 gaggaacagg attttttcga catggcctgg gcctacctgc aaagggccgc tgccgacaat
     6481 gtcgtgcaca cggagatgtt cttcgaccct cagacgcata cggcgcgcgg cgtgagcatg
     6541 gaaaccgtga tcaccggcct gcaccgcgcc tgcacccagg ccggcccggc gctgggcgtc
     6601 agcgcctcgc tgatcatgtg cttcctgcgc cacctgagcg agcaggaggc gttcgagacg
     6661 ctggagcagg cactgccgtt ccaggacaag ttcatcgggg tcggccttga ctcgagcgag
     6721 gtgggccatc cgccggaaaa attcgctcag gtgtttgcgc gctgcggcga gctcggcctg
     6781 caccgcgtcg cccacgcggg cgaggaaggg ccgccagcct acgtctggac cgcgctggat
     6841 ttgctgaagg tcgaacgcat cgatcatggc gtgcagtcca gcaaggacgc ggcgctgatg
     6901 gcgcggcttg cgaaggaccg cattccgctc accgtgtgcc cgctgtcgaa cctgaagctg
     6961 tgcgtatttc caaagctcgc cgatcacaac atcggcaagc tgctcgatgc gggcttggtc
     7021 gccaccgtga attccgacga tccggcttac ttcggcggct acatgaacga caacttcgtc
     7081 cagaccttcg ccgccaccgg gctcacggca acgcacgcgt accagctcgc caagaacagc
     7141 ttcgacgcga gtttcatcca tgagtccatg aagcggcgtt acacggactt gctggacgcg
     7201 accttcgccg cgttcgaatg agggggcttc gatccggacc gcgatgacgg ccagagggta
     7261 aaatcgcctc gccgctgtgc cccgcagcgg cgtttttcat tgacccgggg ccatgtagag
     7321 gaccaccatg tacaaaaacc tcgcagccgc gctcgcggcc gcctgttttt tctctcccgc
     7381 tttttcccag agcaagagcc agcccaagcc ccaggagccg ctcaaggtcg gcttcgtcta
     7441 tgtcgcgccg atcaccgagg ccggctgggt gcaacagcac gaacgaggcc gccaggcggt
     7501 cgaagttgcg ttcgccggca aggtcaagac gagctttgtc gagaacgtcg ccgaaggcgc
     7561 cgattccgaa cgcgtcatcc gcgacctggc caagcagggc cacaagctga tcttcacgcc
     7621 cagcttcggc tacatggagc cgacgctgaa agtggccaag gaatttcccg acgtgaagtt
     7681 cgagtcgatc accggctaca agaccgcgcc caacgtcgcc gtggccaatg cacggtacta
     7741 cgagggccgc tacctggcgg gcattgccgc cgggcgcatg accaagacca acgtggccgg
     7801 ttatgtggcc ggtttcccga tccccgaagt gctgcagggc atcaacgcgt tcacgctggg
     7861 catgcgttcg gtcaacccga aggcgcaggt caaggtggtg tggctcaacg cctggttcga
     7921 tccgccgcgc gagcgcgacg cggccatgac cctgttcaac caggacgtgg acgtgatcgc
     7981 cttccacacg gcgtcgaccg cggtgatggc tgccgcccag gagcgcggca agatggccgt
     8041 cgcctatcac tccgacatgc gcaaggccgc gcccgacgcg cagatcgtgg ccgtcaccca
     8101 tcactggggc gactactacg ttcgccgcgt gcaggcgctg ctggagggac gctggcagac
     8161 cggcagtttc tgggggggcg tgaagcacgc catggtgcgg gccggcgact tcggacccaa
     8221 ggtgccgaag gccgtgcgcg acgaagtgct ggtgcgccag ggcgagatcg cgaagggcaa
     8281 gctgttgccc ttcagcggcc cgatcgtgga caacgagggc aagcaggtgc tcgccaaggg
     8341 caaggccatg acagacgagc agatcctgtc catgaacttt ctcgtagccg gggtgcaggg
     8401 caaggtcgca aaataggctg atgcaagcct ttcgttccgc gatcctgcga tttgccgacg
     8461 accagtcagc cgtctatgaa caggacggcc tgctggtgat cgggccggat gcgcgagggc
     8521 ggcaggtggt gcacgccgtc ggcgattacc acagcctcgc gcccgcctac ccgggcctgg
     8581 ccgtggagca cttgcccggg cgcatcatcg cgcccggttt catcgacctg catatccact
     8641 tcccgcagac cgacttgatc ggctcgccgg cggcgggcct gctgccttgg ctggagcagt
     8701 acacgtttcc gcacgaatcg cgtttttccg agaatgcgca tgcgcaggaa ctggcgatct
     8761 tctttctcga tgagctgctg cggcacggcg tcaccacggc gctggcgttc gccagttcgc
     8821 atccgccgtc ggtcaatgcg ctgatggctg aagcgcagcg gcgcggcctg cggctgatcg
     8881 cgggcaaggt gctgcaggac cggcattcgc cggacggcgt gcgcgacgaa accgagcagt
     8941 ccctcgtgga cagcgaagac ctgatccgca aatggcacgg ggtggaccgg ctgggctatg
     9001 cgatcacgcc gcgctacgcg cccagctgca gcgaagccca gttgctcggc gcgggcgagc
     9061 tggcggcgcg ttatcccgag gtctggatcc agtcccatgt ggccgagaac ctcgaagaag
     9121 tgaagtgggc gcgcgagctg ttccccgcat cgcgcagcta cctggcgatc tacgacgact
     9181 tcggattgat gcgcgagcgg gcgatctacg cccactgcat ccatttcgac gacgaggacc
     9241 gggcgatgat gcgcgccacg ggcgcggcgg ccgccgtcag ccccaccagc aacctgttcc
     9301 tgggcagcgg attcttcgat tacgagggcg cgcagcgcgc cggcttcaag tacggcctgg
     9361 ccagcgacgt cggcggcggc accagcttca gcccgttcca caccatgctc gcagcctatt
     9421 acgtgggccg cgaggggcgc agcaagccgg gcctgtcgct gtcgccgcag cacctgtggt
     9481 ggcagcacac cgcgggcgcc gcgcaggcgc tggggctgca gggcgtgatc ggcaacctgc
     9541 agcccggctg cgaggcggat ttcatcgtgc tggatcccgg tgccacgccg cttctggcgc
     9601 gcaggacagc cagtgccggg accctggccg aactgctgtt cgcgctgatc gtgctggggg
     9661 atgatcgtgt cattcaggaa gtgtcaattt cgaaaaacac agaggaggtc cgatcatgag
     9721 tgatgaggag cattttgctt tgaagtcatt ttcatatttg gaccccaaat acactcgcga
     9781 tcgatttcat gcggacattg ctgatgcgga tcccgcactg ctaccgttca gggtaacggc
     9841 cgcgtgttca agggccgcct tggcagagat ggcaccaact acacgtcctc atcattgaat
     9901 atcgtacgag aatcacgccc gacaatgaag gtaccgaatt gggttgacca ggcgtgagca
     9961 tcattgtcaa tcgattcaag cggttcacac gcaaccccgc tggccgcaaa cattttgctc
    10021 gaagcagcat tggagtcgac gtgatgtgtc ttctcccgta gatatattac gcttctaatg
    10081 ccggcctgaa taatcgcttg ggcacaacgc ggacaaggaa attgcgtcgt gtatatcgtt
    10141 gcgtttctca atggcagaac tacgcaattg agaattgcat ttatttctgc gtgcacgaca
    10201 taactgtgtc gcgagcgaaa tgcgtcatcg tcgttgtcag accagtacac cggatccgcg
    10261 tcatcgcacc cacgcggcaa tccgttgtaa cctacgccaa taattctgtt gtccggggtt
    10321 acgatgcatg caccgttacg agtccgatga tctttggatc gcgcagatgc cagcaaagca
    10381 actcccatga acattgactg ccagccaatc aaagcgcggg ggagggggag tcggggggta
    10441 tcgtcgctca tgtgttcaac aaacttgatt gcgtgcttgt gtttcgcttg ggtccgatga
    10501 tgtcctctac taatccgaac cccatcctcc cacccgcccc cggagctgat tgccacttcg
    10561 caatctgaca gtttgtatta ggcgcaagta attagacaga atatattagc actagggaca
    10621 gcttgcgtct gcctcggcgc tgtgtacggc gtgaccctgc ccaaggcctg agaaaccggc
    10681 gaacccggtc tgcggcccga gcgcgaggcc ggctatttct gaagcgccag cgccacccgc
    10741 gccgccgcca ggtcccagcc cgcccagccg cagctcttga acaggatcgg cgcgcgcgta
    10801 ctggccggcg cggtccccag caccacttcg cgcagcgtcg gaaagccgct cacgtccagc
    10861 cccgcctgca gcaggtcgcc cgcttcatga acggcctgcg gcgtatcgac caccacccgc
    10921 ccatgggtcg cgcaatggcg gcacagcccg gagtccagct cgaccatgga cggcgtgaaa
    10981 gcgccgaccg cggcgatgaa ggcgtcttcg cgcggcaggg catggagcac cacgtcattg
    11041 gccggcgtgc aggtcacgat cagcgggcag tcggcgacgg cctggttggc gtccaccacc
    11101 gtgccggcgt gcaatcccag ggcgcgcgca tagccggcca gcgcctgcgc gctcgcttcg
    11161 ctgcgagagg cgatgaacac ctgccgcaag ggcaggccct gcgcgaaggc ttccaggtgg
    11221 gcccgtccct gcgccccagc gcccacgatc aatagcgggc cttccttcct gggcgccagg
    11281 cactgcgccg ccagcagcga gaccgccgcg gtgcggcgcg ccgtgaccgt cggcccgtcg
    11341 aggatcagcg tgcgctcgcc ggtggcgacg tcgaacacca cgacgtcgcc ctggatggcg
    11401 ggccgccgcg tgcccacgtt ggccggcgtg aacgtgatca acttggcaat ggcgatgcgc
    11461 cgatccaacg ccggcatgac gaacaggctg ccgccgcccg gcaacggctg caccagccgc
    11521 tcgggcaccc gcaccgaggc gtcggccagc agctcgcgca gctgcgccac cagcgccggg
    11581 aaggccaggc aggcggcggt ttgcgtcggc tcgagcaggc gtgtcatggc gtttccggtg
    11641 cgaagctgga gtagcgagat tttgtcctac agcatgacgc gaaaaaaaag gggttgcggg
    11701 ccaggatagt cattcctaac atgaatccaa ccccaaggag ttcctcatgc tgtcccgcct
    11761 gcccgaaaga cctgccgcct cgcgcgacag gctgttccgg gcggtgctgg tggcgctggc
    11821 catcggacaa ctggtggcct tctggatgct gtgcagccac caggttcgca aggcccagat
    11881 gcgcgacgtc ggcctggcgg tacagcgcgt cgccatagcc gactgcctgc agtacattcc
    11941 ccgggccacg ctcaacagtt gcatcaaccg ggtcgacccc agccgcgaaa gcagcgctgt
    12001 gatggccacc ggccacaagc cgacagcggc gccggacgcc acgcgcgcga cgatgagcag
    12061 cggcacgccg gtcagcgtct cctaccagtg atccccttcc cgacgcacaa gcatcgtctg
    12121 aaagactgat gcctcagcgg gaacgcgtcc gacagcgacc gggtatgcgc accgactgtc
    12181 ccggcacggc gccgcgccta atattcgatc atggaaaaaa gggacgaacc caccatgaac
    12241 aataccgttg cccattctgg ttttccctgg agcgatccga tgctcaagtc gctggacgct
    12301 tcggacgact tcgatgcgat gcccgacgcg acgcgctact gtcccaatca gttgctgtcg
    12361 gctttctgcc tgttgatggc ggggcacggc cggtccatca gcacggccat gatgctcggc
    12421 gaccgggaat acgccatgtg gcaactggcc cgggcccata cgctggacga tcgcgagctg
    12481 cgcagcgtcg cggccaggtt gttcggctat ttcgacgacg tccacgcccc ggtgcccgcc
    12541 ggcctgcacg attgaggctg tcatgccagg ctcgtagcaa atgacacagt ggctgtcatg
    12601 ccgggcttga tccggcatcc aggaagtcat ggattgcggg tcaagcccgc aatgacaact
    12661 catgggaaac acggcagatg acaccaacgc ttccttcgtg ggaaaccctt gacaccggcg
    12721 acaccctgcc gccgctggag ctgcccccta tcacccgcct ggcgctcgcg ctgtactgcg
    12781 gcgcctcggg cgaccacaac ccgatccacg tggacaccga cttcgccaag tccgccggca
    12841 tgccggacgt gttcgcgcac ggcatgctgt cggccgcgtg gctgggccgg ctgctgacga
    12901 actgggtgcc gcaatcggcg atccgctcgc tggacgtgcg cttcgcggtc atcacgcacg
    12961 tgggcgaacg catcacctgc accggcaagg tgaccgagaa attcgaagac cagggccggc
    13021 gctatgcgcg ccttgccctc accaccacca accaggacgg cttggtcaag ctgagcggtg
    13081 aggcactg
//
LOCUS       DASWIY010000326         3446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2810475, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000326 DASWIY010000000
VERSION     DASWIY010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2810475"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..669
                     /locus_tag="VGA59_11690"
     CDS             172..669
                     /locus_tag="VGA59_11690"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9720386.1"
                     /translation="MSSNIVRVTHRFNTSAQRVFDAWLTPALAARFLFTTRTGNILHC
                     EIDPQIGGGFTVTDRRPVTDGEESFFEARHRGVYVEIDRPGRLAFDLSVEPSFETPTR
                     VTIDVVPMGANMCDLVLTHDMGEGDNARAMAERSRQGWDNMLRQLDKVLSTRSWGGFR
                     TPGNH"
     gene            905..1135
                     /locus_tag="VGA59_11695"
     CDS             905..1135
                     /locus_tag="VGA59_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720387.1"
                     /translation="MQFVQDSFDAGSVVAAWLSPGTLGQLEPVLRRLMAGKQLFVKQP
                     DGTYRPRGSELGLAHYFEFSDLQAPALRNVNY"
     gene            complement(1274..1681)
                     /locus_tag="VGA59_11700"
     CDS             complement(1274..1681)
                     /locus_tag="VGA59_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9720388.1"
                     /translation="MSNASLPPSVLSHVSLGTNDYARAKPFYDAVLAALQIGCVMDFP
                     GGAGYGRAFPEFWIQSPHDGGRASAGNGVHVSFLANSVEEVKAFHAKALELGATDEGA
                     PAYRPEYTLNYYAAFVRDLDGNKIEAMLMVPGK"
     gene            complement(1745..2353)
                     /locus_tag="VGA59_11705"
     CDS             complement(1745..2353)
                     /locus_tag="VGA59_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9720389.1"
                     /translation="MTAVAVIDFETTGLSPATGDRATEIAVVLLEGDRVVDRFQSLMN
                     AGVRISAFIEAYTGISNAMIAGAPPAATVMADASRFVGGRPMVAHNASFDRRFWMAEL
                     DRVDRPAAEPFACTLLLSRRLYPHAPNYRLGSLAAFHALPSSGRAHRAMADAETAAAL
                     LGRIRSDLRFRFGVAEPRHALLMKLQACSRKALPRAIADSAP"
     gene            complement(2361..2807)
                     /locus_tag="VGA59_11710"
     CDS             complement(2361..2807)
                     /locus_tag="VGA59_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEX9720390.1"
                     /translation="MKVHVLRLAPGDDLRAKLEAAFAELSRTQGITAACIVSAVGSLS
                     QAILRYADRSGGSETNGPLELLMLSGTLSADGAHLHASVADSHGAVKGGHLMPGCIVR
                     TTCEVVIALLPGWEFRRELDVATGFRELQAEPEMPAPQPFPRGKGS"
     gene            complement(2804..>3446)
                     /locus_tag="VGA59_11715"
     CDS             complement(2804..>3446)
                     /locus_tag="VGA59_11715"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEX9720391.1"
                     /translation="NNHVSVDLPQFGINIAAPSMLRFWTMLAHFHGQIWNAAHPAQSL
                     GVSEPTVRKYLDTLTQTLMIRQLQPWYENLGKRQVKSPKIYFRDTGLLHTLMGVKTLP
                     ELATHPRAGASWEGFALEQVLRIAQPDQAYFWATHQGAELDLLMFKGRQRVGVEFKRA
                     DAPKVTQSMRIAMQDLKLDALYVVHPGLHRFKLADGIEAVPLWAMLPAAGVLS"
BASE COUNT          603 a         1149 c         1125 g          569 t
ORIGIN      
        1 tgggttcgac catgccccct gtcattgcgg gttcgaccac gccccctgtc attgcgggct
       61 cgacccgcaa tccatgaatt ctgagtcatg gatcccgggt caagcccggg atgacagcct
      121 ttgacatctc tgggacttga caccacgcag ttcgcggcag ataaacggtc gatgagcagc
      181 aatatcgtgc gtgtgacgca tcgtttcaac acgtccgcac aacgcgtgtt cgacgcgtgg
      241 ctcacgccgg cgctggcggc gcgcttcctg ttcaccaccc gcaccggcaa catcctgcat
      301 tgcgagatcg atccgcagat cggcggcggc ttcaccgtga ccgaccggcg gcccgtgacc
      361 gatggcgaag agagtttttt cgaggcgcgg caccgcggcg tctatgtgga gatcgaccgg
      421 cccggacggc tggccttcga cctcagcgtc gagccctcgt tcgagacgcc cacgcgcgta
      481 acgatcgacg tcgtgccgat gggcgccaac atgtgcgacc tggtcctcac gcacgatatg
      541 ggcgaaggcg acaacgcgcg tgccatggcc gagcgctccc gccagggctg ggacaacatg
      601 ctccggcagc tggataaggt gctgagcacc cgcagctggg gcggcttcag gacccccgga
      661 aaccactgat ctcggccacc ggccaccccc gctaaaccga aaggccgcaa caaaccagct
      721 gcgtgaggca gctgttttgc gcgcatacaa cggattcgta aaagtggcgg ccagaacggc
      781 cgtttgtcat gccctggact tcccaaaatt gctgcagcgc gcaaaatccg ccccaaggca
      841 gcagtcaggc caaaaaatag accgcggtaa gggagcccgt ccaatggatt tgcaccggag
      901 tgccatgcag tttgttcaag actctttcga cgccggcagc gtggtcgctg cctggctgag
      961 cccgggcacg ctgggccagc tcgagcccgt gctgcgccgg ctgatggccg gcaaacagct
     1021 attcgtcaag cagcccgacg gcacctaccg cccccgcggt tccgagctgg ggctggcgca
     1081 ctacttcgag ttcagcgacc tgcaggcgcc cgccctgcgc aacgtgaatt actgagcgac
     1141 gttcttctgc gtgggctttt gctcgccgag cttgttgaaa tcgcgaccca ttctgggagg
     1201 gcgaccagcg cctggctgtc ggactgcgtc gcatccggcg tagggcccag cttgcccggc
     1261 ccagccccgt ccgctacttg cccggcacca tcagcatcgc ctcgatcttg ttgccgtcga
     1321 ggtcgcgcac gaatgcggcg tagtagttga gcgtgtactc cggccgataa gccggtgccc
     1381 cttcgtcggt ggcgcccagc tcgagcgcct tggcatggaa ggccttcact tcctcgaccg
     1441 aattggcaag aaatgaaacg tgcacaccgt tgccggcgct cgcccggccg ccgtcgtgcg
     1501 gcgactggat ccagaactcc ggaaaggcgc ggccgtaacc ggcgccgccc gggaagtcca
     1561 tgacacagcc gatctgcagc gcggcgagca ccgcgtcgta gaagggcttg gcacgtgcat
     1621 agtcattggt gccgagcgac acatgggaca gcacgctggg aggaaggctg gcgtttgaca
     1681 tcggtgtacc tcgtgaagga acgaacggag cggatattgg acacccgcct gccgccggct
     1741 tgtgtcaggg tgccgagtcc gcgatcgcgc gcggcagtgc cttgcgcgag caggcctgca
     1801 gcttcatcag cagcgcgtgc ctcggctcgg ccacgccgaa ccggaaccgc aggtcgctgc
     1861 ggatgcggcc cagcaatgcg gcggcggtct ccgcgtcggc catcgcccgg tgggcgcgcc
     1921 ccgaagacgg cagcgcatgg aaggcggcca gcgagccgag ccggtaattc ggcgcatgcg
     1981 ggtacagccg ccgcgacagc aacagcgtgc aggcaaacgg ctcggccgcg ggccggtcca
     2041 cgcgatcgag ttccgccatc cagaagcggc ggtcgaacga tgcgttgtgc gccaccatcg
     2101 gccggccgcc gacaaagcgg ctggcatcgg ccatcaccgt cgccgccggc ggcgcgccgg
     2161 cgatcatcgc gttgctgatg ccggtgtagg cctcgatgaa ggcggagatg cgcacgccgg
     2221 cgttcatcag gctctggaag cggtcgacca cccggtcgcc ctccagcagc accaccgcaa
     2281 tctccgtcgc ccggtccccc gtcgcgggcg agagcccggt ggtttcgaag tcgatgacgg
     2341 cgacggcggt cacaaaggct tcaactcccc ttgcctcttg gaaagggctg gggcgcgggc
     2401 atctccggct cggcctgcaa ttccctgaag ccggtcgcca catccagctc gcggcggaac
     2461 tcccagcccg gcagcagcgc gatcaccact tcgcaggtgg tgcgcacgat gcagccgggc
     2521 atcaggtgcc cgcccttcac ggcgccgtgg ctgtcggcca cgctggcatg caggtgcgca
     2581 ccgtcggcac tgagcgtgcc cgacagcatg agcagctcca gcggcccgtt ggtctcgctg
     2641 ccgccggacc ggtcggcata gcgcaggatg gcctgcgaca ggctgcccac cgcggaaacg
     2701 atgcaggccg cggtgatgcc ctgcgtgcgc gacagttcgg caaacgccgc ctccagcttg
     2761 gcgcgcaggt cgtcgccggg cgcgaggcgc aggacatgga ctttcatgac aacactcccg
     2821 cggcaggaag catggcccac agcgggacgg cctcgatgcc atcagccaat ttgaatcggt
     2881 gcaagccggg gtgcacgacg tagagcgcgt ccaatttcaa gtcctgcatg gcaatgcgca
     2941 tggactgcgt caccttgggc gcgtcggcgc gcttgaattc cacgcccacg cgctgccggc
     3001 ccttgaacat cagcaggtcc agctcggcgc cctgatgagt ggcccagaag taagcctggt
     3061 cgggctgcgc gatgcgcagc acctgttcga gcgcgaagcc ttcccagctg gcgccagcgc
     3121 gcggatgtgt ggccagttca ggcaaggtct tgactcccat cagcgtatgc agcaggccgg
     3181 tatcccgaaa gtagattttg ggcgacttca cctgccgctt gcccagattt tcgtaccagg
     3241 gctggagctg ccggatcatc agcgtttgcg tcagtgtatc gaggtacttg cgcaccgtcg
     3301 gctccgacac gcccagggac tgcgccggat gggcggcatt ccagatctgg ccatggaaat
     3361 gggcgagcat ggtccagaag cgcagcatcg atggtgccgc aatgttgatg ccgaactgtg
     3421 gcagatccac gctcacgtgg ttgttg
//
LOCUS       DASWIY010000327         6205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_590137, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000327 DASWIY010000000
VERSION     DASWIY010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590137"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /gene="sucC"
                     /locus_tag="VGA59_11720"
     CDS             <1..214
                     /gene="sucC"
                     /locus_tag="VGA59_11720"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485552.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HEX9720392.1"
                     /translation="NIFGGIMKCDTIASGIVAACKAVNLSVPLVVRMKGTNEEMGKKL
                     LAESGLPIISADTMAEAAQKVVAAVR"
     gene            275..1168
                     /gene="sucD"
                     /locus_tag="VGA59_11725"
     CDS             275..1168
                     /gene="sucD"
                     /locus_tag="VGA59_11725"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX9720393.1"
                     /translation="MSIYINKDTKVITQGITGKTGQFHTEKCQEYANGKNCFVAGVNP
                     KKAGEKVFDIPIFASVKEAKAQTGATVSVIYVPPAGAAAAIWEAAEADLDLAICITEG
                     IPVRDLLEVRNKIKAKVAAGGKDTLLLGPNCPGLITPDEIKIGIMPGHIHRKGRIGVV
                     SRSGTLTYEAVAQLTELGLGQSSAVGIGGDPINGLKHIDIMKAFNDDPDTDAVIMIGE
                     IGGPDEADAARWCKDHMKKPIVGFIAGVTAPAGKRMGHAGALISGGADTAEAKLAIME
                     ECGFKVTRNPSEMGKLLKALL"
     gene            1267..1974
                     /locus_tag="VGA59_11730"
     CDS             1267..1974
                     /locus_tag="VGA59_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX9720394.1"
                     /translation="MEAMLHAPFWIAVGQIILIDILLGGDNAVVIALACRGLPPKQRK
                     LGIIYGTAGAIVLRVILIAFALALLAVPYLKLVGGLLLIWIGVKLLVPADDEEDHLNI
                     NTSDKLWGAVKTVIVADLVMSVDNVLAIAGAAENAGKNQLFLVIFGLLVSIPIIVAGS
                     QLVLKLMDRFPIIITLGAMLLGWIAGQMMYTDPATHAHLPEGKVWEYATAVFGALLVL
                     GIAKIVQARRAGKPDPA"
     gene            2162..4942
                     /locus_tag="VGA59_11735"
     CDS             2162..4942
                     /locus_tag="VGA59_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9720395.1"
                     /translation="MGKSSSAKRRQFWRTDWFVAGLVILAVVVLHQATDVIGTAERRF
                     YDFASTSSGRQPSDRIAVIAIDDQSIANIGRWPWPRDVHAQLIDQLSAAKAKTIVYTT
                     FFFEPQTDRGLVFIRRIKEALGSVAEGQGGPAEQLGKVIEEAEQTLDTDAKLAASMAR
                     AGNVLLPSVYELGEPQGRADGNLPAYVARSAIDESSGFSVPAIRGQYPIASIGSAASG
                     IGHLNQLSDVDGAVRDDPLLVNYYGKAVPSMALLAAARSLNLGPGDIKLNPGESVQIG
                     KLRVKTDDSARMLPQFYKGRDGKPAFAVDSFYDVLSGKIPAGKYADKIVLIGATAAGV
                     GTTFAAPGYAGLSPAEMMAHITSSILGEHFIVQPAWATWAALAAFLLVAAYLIALLPR
                     LSAGMGAAATAALFLLLIGSEFVLLSSASLWLNLVFPAALLVIGHLALTTKRFLVTEA
                     GKLKSDDESAETNRMMGLALQGQGQLDASFDRFRRVPMGETVMENLYNLALDFERKRQ
                     FNKAQTVYEHMAAWNKDFKDLQSKLNRARNLSETVILGGGGSRSGGTMLLDGGAVEKP
                     MLGRYQVEKELGKGAMGVVYLGKDPKIGRVVAIKTMALSEEFEGEELTDARERFFREA
                     ETAGRLQHQNIVTIFDAGEEHDLAYIAMEFLKGKDLVDFCKDGQLLPIPKVVAIVIRV
                     AEALAYAHKQNVVHRDIKPANIMYEFESDTVKVTDFGIARITDSSKTRTGLVLGTPSF
                     MSPEQIAGKKVDGRSDLYSLGVMLFQMLSGVLPFRGESMAELMYKIANEDAPDVRSIR
                     KDIPERLANIVALSLSKRSETRYQDGGQFAADLRAVLAEMSGAPAASIASPVRREPVP
                     ATAQDQTVILGPGGAGLAFEPTVVGRPVAPGYDAAQASEAGNAFAKTDVISRPGAPGS
                     PAAGARKTDPEA"
     gene            4954..5739
                     /locus_tag="VGA59_11740"
     CDS             4954..5739
                     /locus_tag="VGA59_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX9720396.1"
                     /translation="MNYEFCIRTDPGLARENNEDSVTLDEPARLGILADGMGGYNAGE
                     IASGMATTFIKSELGRWLSQAGRHANAREVRRAMEICVDNANRSIFNAANSNPQYSGM
                     GTTLVLGVFQDGRLMLGHIGDSRCYRLRGNEFTQITKDHSLLQEQMDAGLITPEQAAT
                     SSNKNLVTRALGVEDAVLLEVNEHKVEPADIYLMCSDGLSDMVDDEGIGKILASDAPL
                     EQRVAQLIDTANANGGRDNISVLVAQANTGSKKRGLISRLLGK"
     gene            5789..>6205
                     /locus_tag="VGA59_11745"
     CDS             5789..>6205
                     /locus_tag="VGA59_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX9720397.1"
                     /translation="MPKMIVSIDGVVIKEVQLTKDRTTLGRRPYNDIVIDNLAVSGEH
                     AVLQMAGNEVYLEDLNSTNGTYVNGKAVKKQLLQNSDTVEIGKYKIKYINEAAGPSFE
                     KTMIMKSGSAGIVPPAPVRPPVGAAPAQAPAPAADLA"
BASE COUNT         1221 a         1973 c         1991 g         1020 t
ORIGIN      
        1 caacatcttc ggcggcatca tgaaatgcga caccatcgcc agcggcatcg tcgcggcctg
       61 caaggcggtg aacctcagcg tgcccctggt ggtgcggatg aagggtacca acgaagagat
      121 gggcaagaag ctgctcgccg agtccggcct gccgatcatc agcgcggaca ccatggcgga
      181 agcggcccag aaggtcgtgg cggcagttcg gtgactttgc gggacagatc ttcagctctg
      241 tccccaactg actaaagaca aatcaggacg aatcatgtcg atctacatca acaaagacac
      301 caaggtcatt acccagggca tcacgggcaa gaccggccag ttccacaccg aaaagtgcca
      361 ggaatacgcc aacggcaaga actgcttcgt cgccggcgtg aaccccaaga aggccggcga
      421 gaaggtcttc gacattccga tcttcgcgtc ggtgaaggaa gccaaggcgc agaccggcgc
      481 gacggtgtcg gtcatctacg tgcccccggc gggcgcagcg gccgccatct gggaagccgc
      541 ggaggccgat ctggacctgg cgatctgcat caccgaaggc attccggtgc gcgacctgct
      601 cgaggtgcgc aacaagatca aggccaaggt ggccgccggc ggcaaggaca cgctgctgct
      661 gggacccaat tgcccgggcc tgatcacgcc cgacgagatc aagatcggca tcatgcccgg
      721 ccacatccat cgcaagggcc gcatcggggt ggtgtcgcgc tccggcacgc tgacctacga
      781 agccgtcgcg cagctcaccg agctcgggct gggccagtcc agtgccgtgg gcatcggcgg
      841 cgacccgatc aacggcctca agcacatcga catcatgaag gccttcaacg acgatcccga
      901 caccgacgcc gtgatcatga tcggtgaaat cggtggcccc gacgaagccg atgccgcgcg
      961 ctggtgcaag gaccacatga aaaagccgat cgtcggcttc atcgcaggtg tcaccgcgcc
     1021 ggccggcaag cgcatgggcc acgcgggtgc gctgatctcc ggcggggccg acacggccga
     1081 agccaagctg gcgatcatgg aagagtgcgg cttcaaggtc acccgcaacc cgtccgagat
     1141 gggcaaactg ctcaaggcgc tgctctagcg cgccgctgcg gttacccaca acagacgggc
     1201 cgactgtcgt cgcggaaaat aggtggtcct gttttttttg tctgaacggt cgttcgagag
     1261 tcccgcatgg aagcaatgct gcacgccccc ttctggatcg ccgtcggcca gatcatcctg
     1321 atcgacatcc tgctgggcgg cgacaacgcc gtggtgatcg ccctggcgtg tcgggggctg
     1381 ccgcccaaac agcgcaagct gggcatcatc tacggcacgg ccggcgccat cgtcctgcgc
     1441 gtcatcctga tcgccttcgc cctggcgctg ctggccgtgc cctacctgaa gctggtcggc
     1501 ggcctgctgc tgatctggat cggcgtgaag ctgctggtgc ccgccgacga cgaagaagac
     1561 catctcaaca tcaataccag cgacaagctg tggggcgccg tcaagaccgt gatcgtggcc
     1621 gacctggtga tgagcgtgga caacgtgctg gcgatcgcgg gcgcggctga aaacgccggc
     1681 aagaaccagc tcttcctggt gatcttcggc ttgctggtca gcatccccat catcgtcgcc
     1741 ggcagccagc tggtgctcaa gctcatggac cgcttcccga tcatcatcac gctcggggcg
     1801 atgttgcttg ggtggatcgc aggccagatg atgtacaccg acccggccac gcacgcccac
     1861 ctgcccgagg gaaaggtgtg ggaatacgcg actgcggtct tcggcgcctt gctggtgctg
     1921 gggattgcca aaatcgttca ggcccgtcgc gctggcaagc ccgaccctgc atgaccgccg
     1981 gcgtacgctt gatcgctaaa ttgcaacgaa acgtttggtg ggcgtgacaa agtgccttgt
     2041 ccgctgctga gcggtctgtt aacgtccccg tgaggaggcc tgcacccttt ttctggcgtc
     2101 gcgggtgtga aatgccgcgc aagccgcgga acggaacgtt atcctggaca aggggccggc
     2161 agtgggcaaa tcatcatcgg cgaagcgtcg ccagttctgg cgcacggact ggttcgtggc
     2221 cgggctggtc atcctcgccg tcgtcgtcct gcatcaggcc acggatgtga tcggcacggc
     2281 cgagcggcgc ttctatgact tcgccagcac cagcagcggc cggcaaccgt ccgatcgcat
     2341 cgccgtcatc gccatcgacg accagagtat cgcgaacata ggccgctggc cctggccgcg
     2401 cgatgtccat gcccagctga tcgaccagct ttcggcagcc aaggccaaga ccatcgtcta
     2461 cacgactttc ttcttcgagc cccagaccga tcgcggactg gttttcattc gcaggatcaa
     2521 ggaagccctc ggatccgtgg ccgaaggcca gggcgggccg gcagagcagc tgggcaaggt
     2581 catcgaggag gccgaacaga ccctcgatac cgacgccaag ctcgccgcca gcatggcccg
     2641 cgcgggcaac gtgctgctgc cttccgttta cgaactgggc gaaccgcagg gccgggcgga
     2701 cgggaatctt cctgcctatg tcgctcgcag cgcgatcgac gaaagcagcg ggttttcggt
     2761 gccggcgatc cgcggccagt atccgatcgc atccatcggc agtgcggcat cgggcatcgg
     2821 ccacctgaac cagctcagcg acgtcgacgg ggccgtgcgc gacgacccgc tgctggtcaa
     2881 ctactacggg aaagccgtgc cctcgatggc gttgctggcc gccgcccgca gcttgaacct
     2941 gggccccggc gacatcaagc tcaacccggg cgagtccgtc cagatcggaa aattgcgcgt
     3001 gaagaccgac gacagcgcca ggatgctgcc gcagttctac aagggacggg acggcaagcc
     3061 cgcgttcgcg gtggactcct tctacgacgt gctgagcggc aagattccgg ccggcaagta
     3121 cgccgacaag atcgtgctga tcggcgccac ggccgcggga gtgggcacca cgtttgcggc
     3181 accgggctat gccgggttgt cgccggccga aatgatggcc cacatcacgt cgagcatcct
     3241 gggcgagcac ttcattgtcc agcccgcctg ggcgacctgg gccgcgctgg cggcattcct
     3301 cctggtggcc gcctacctga tcgccctgct gccccggctg tcggctggca tgggggccgc
     3361 cgccacggcc gcgctgttcc tcctgctgat cggaagcgag ttcgtccttt tgtcgagcgc
     3421 gtcgctctgg ctgaacctcg tgttcccggc cgccctgctg gtgatcggcc atctggcgct
     3481 caccaccaag cgcttcctgg tcacagaggc cggcaaactg aaatcggacg acgagtccgc
     3541 cgagaccaac cgcatgatgg ggctggcgct gcaaggccag ggccagctcg atgcatcgtt
     3601 cgaccgcttc cggcgcgttc ccatgggcga aacggtcatg gagaacctgt acaacctcgc
     3661 gctcgatttc gagcgcaagc gccagttcaa caaggcgcaa acggtgtacg agcacatggc
     3721 cgcgtggaac aaggatttca aggacctgca atccaagctc aatcgcgcca ggaacctgtc
     3781 cgaaaccgtc atcctcggcg gtggcggctc gcgttccggc ggcaccatgc tgctcgatgg
     3841 cggcgccgtg gaaaagccca tgctgggccg ctaccaggtt gagaaggaac tcggcaaggg
     3901 cgcgatggga gtcgtgtacc tcggcaagga tcccaagatc gggcgcgtgg tggcgatcaa
     3961 gaccatggcc ctttccgaag agttcgaagg cgaggaattg accgacgcgc gcgagcgctt
     4021 cttccgcgag gccgagacgg ccgggcgcct gcagcaccag aacatcgtca cgatcttcga
     4081 cgccggcgag gagcacgacc tggcctacat cgccatggag ttcctcaagg gcaaggacct
     4141 ggtggatttc tgcaaggatg gccagctgct gcccattccc aaggtggtgg ctatcgtgat
     4201 ccgggtggcc gaagcgctgg cctatgccca caaacagaat gtggtgcacc gggacatcaa
     4261 gccggccaac atcatgtacg agttcgaaag cgacacggtg aaagtgacgg acttcggcat
     4321 cgcccgcatc accgattcaa gcaagaccag gacggggctg gtcctgggca cgcccagctt
     4381 catgtcgccc gagcagatcg ccggcaagaa ggtggacggc cgctccgacc tctactcgct
     4441 gggcgtcatg ctgttccaga tgctcagcgg cgtgcttccg ttccggggcg agtcgatggc
     4501 ggaactgatg tacaagatcg ccaacgagga cgcgccggac gtgcgcagca tccgcaagga
     4561 catccccgag cggctcgcca acatcgtggc gctttcactg agcaagcggt cggagacccg
     4621 ctaccaggat gggggccagt tcgccgccga cttgcgcgcg gttctggccg agatgtcggg
     4681 cgcgccggct gcctccatcg cgtcgccggt gcggcgcgag ccggtaccgg ccacggccca
     4741 ggaccagacg gtgatcctgg gccccggagg cgccggactc gcgttcgaac ccaccgtcgt
     4801 cggcaggccc gtggcacccg gctatgatgc ggcgcaggca tcggaggcgg gaaatgcttt
     4861 cgccaagacc gatgtgatca gcaggccggg cgcacccggc tcccccgcgg cgggggccag
     4921 gaagaccgat ccagaggctt gaccccgcaa tttatgaatt acgagttttg catacgcacc
     4981 gacccgggtc tcgcgcgcga gaacaacgag gattcggtga ccctcgacga acccgcccgc
     5041 ctgggcatac tggccgacgg catgggcggt tacaacgccg gggaaatcgc cagcgggatg
     5101 gctaccacct tcatcaagtc ggaattgggt cgctggctct cgcaggctgg acggcacgcg
     5161 aacgcgcgcg aagtgcgccg ggcgatggaa atctgcgtgg acaatgccaa ccgctcgatc
     5221 ttcaacgccg ccaactcgaa cccccagtac agcggcatgg gcacgacgct ggtgctgggc
     5281 gtgttccagg acggccggct gatgctgggc cacatcggcg attcgcgttg ctaccggttg
     5341 cgtggcaacg aattcacaca gatcaccaaa gaccactcct tgctgcagga gcagatggac
     5401 gccggcctga tcacgcccga gcaggccgcg acctcgagca acaagaacct ggtgacgcgg
     5461 gcgctcggag tcgaggacgc agtgttgctg gaagtcaacg aacacaaggt cgagccggcc
     5521 gatatctacc tgatgtgctc ggacggcctg tcggacatgg tggatgatga gggcattggc
     5581 aagatactgg caagcgacgc tccactggag cagagggtgg ctcaactgat cgatacggcc
     5641 aatgccaacg gagggcggga caatatctcc gtcctggtgg ctcaggccaa caccggttcg
     5701 aaaaaacgcg gcttgatttc cagattgctg ggaaaatagc ctttggtatt agttttctga
     5761 cacgagttac ataagacagg agcggaccat gccgaaaatg atcgtttcga tcgacggtgt
     5821 tgtcatcaag gaagtgcagt tgaccaagga ccgtacgacg ctgggtcgcc gtccttacaa
     5881 cgatatcgtg atcgataacc tggccgtcag cggcgagcat gccgtactcc agatggcggg
     5941 caacgaggtc tatctggaag acctcaacag cacgaacggc acctatgtga acggcaaggc
     6001 cgtgaagaag cagctgttgc aaaatagcga cactgtcgag atcggcaagt acaagatcaa
     6061 gtacatcaac gaagcagccg gcccttcatt cgaaaagacc atgatcatga agtccggctc
     6121 ggccggcatc gtgcctcctg cgccggtccg gccccccgta ggcgctgctc cggcccaggc
     6181 gccggcgccg gccgccgacc tggcg
//
LOCUS       DASWIY010000328        10415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3531293, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000328 DASWIY010000000
VERSION     DASWIY010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3531293"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2383
                     /locus_tag="VGA59_11750"
     CDS             <1..2383
                     /locus_tag="VGA59_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HEX9720398.1"
                     /translation="QGAGVPAVTVNSAPKVNFTVFSDGAIKQGLALSDVSFIISKLVP
                     GSNGDPDKWVSYTYRTESTASGTAKTGPGGVPVLASALQATTDTKLTGTAAATQLVYN
                     PDGYYTYTFSTDITDPTKTQGVVFEPNLAHRVAIQLSYTNAAGTTIRVNPYFDFTVGA
                     DGKAVAATARKMVDVATCNGCHDELRLHGGGRKDTQFCVLCHNPSTVDAQSGNNLNLA
                     TMVHKIHSGRELHENGENYTIWGFNASKHDYSEVGFPQPTRNCAACHTGSNPATPQGD
                     NWKSKPAKEACLSCHQSGDTSAWFATHVTALKLGASAAAIPNSSCNSCHGANSTWAPE
                     KAHWVQEMANAALYKGVIESVTVTKAPTAAAAGTISVKYAVINPATGAAYDLAEGCTG
                     AATTDQKGNPIANSCNTNYRWDANLDPTKWTAPPNKFGTFTLGLATNTLAQTLDDTTG
                     TAGGVLPAYRGVKDAGNHYTMEITVPAASKGNARLLLLGSVAEPRLDPMTRTAAGAIP
                     ATKNGDVAYVPVKNDVYEFTATGNGARATSTARRQIVSNDNCNSCHGILGLPTGPNEP
                     ERTGFHKGVRNNSEGCVNCHNANQAGGYTLMTDGSTGPIAGDSQLAQPNTSSFLHESY
                     QAKRFIHGIHGGAKRTYPFTHCLTTGGEYNKDGTSKTGGKPLGDETCVNYPGETLNFT
                     AEVAYPAQLGNCSNCHVNDSWKQDRSVIGSVVFKPVGATALDWLVISPKAATCTSCHD
                     SKAVQTHVTAQGGASFGTVTQDGLLNKGAVFEACTGCHIPGSPIGVDVVHGFK"
     gene            2526..4988
                     /locus_tag="VGA59_11755"
     CDS             2526..4988
                     /locus_tag="VGA59_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HEX9720399.1"
                     /translation="MSATVLFALAIAGCGGGGGGGVDPGTGGTGTPTGSVSEAIAKAA
                     AVPANDTSTNSSAPFAVVQAAGVPAVTVASPPVVNFSVFSDGKVKTGLELSNVSFAIA
                     KLVPGTNGNLDQWVSYVYRTETAAAGVGPGGAPVLAKATQATTDAKPAAAAATQLVFN
                     ADGYYTYTFTTDIKDPAKTNGVTFEPNRTHRVAIQLSYVNAAGETERVNPYFDFTFDA
                     NGNSVPVTDPALTKKMTDVSSCNGCHEKLALHGGGRVDTQFCVMCHNPGTTDANSGNV
                     LTLSTMVHKIHAGRLLHSKLSAGGEDYVIWGYRDTPHDYSEVGFPQDLRNCTKCHTGA
                     NPATPQGDNWKTKASREACLTCHANKEGSEWFSFHKILNNDQDPNAAATLMPTSACAG
                     CHAPGTSIAPERVHWNQNEENAAKYKMNIEDAAYDAATRKVTVKYFLSDPTNGNAAYN
                     LVTPECTGTSTIACSNQTRFGNLRFYLPHQNMIGQPTGTTEFSAHNNGGSSANAFAYK
                     GTNDGSNHYTVQISVPPDSTVSMAFGTARVVSIGQIKEPKLQVKTVSDPRPEVTPREL
                     VNVVVQHTFKDVVLSGTLNPRRAVVSNEKCNVCHGALGTTSGSNTLANAFHGGARNTV
                     EACVICHDPNRMSSTKMTNGLDLNESYQFKRMIHGIHGNSKRTYPFTHGNKVIDAFNK
                     DGTSKTGGLPMLEANLAIGNTSTVENYAAEVAWPGVGLNCNACHVDNSYKLDRGTLGA
                     VVTKPAGVTDPMQWLVISPKAATCTACHDSPAAMGHVTSFGAATFANRTQAESLQIQE
                     TCADCHASGIGFKAVDIVHGQE"
     gene            5072..6037
                     /locus_tag="VGA59_11760"
     CDS             5072..6037
                     /locus_tag="VGA59_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HEX9720400.1"
                     /translation="MRIKRALAAIALGLFGLVATVGAAGVDAEASAACVKCHDEEDMP
                     DNSRHAHAVVTDSRTPTCVTCHGPSTAHRAKPSDTKPDRLFGRKANLPTSELNGACLT
                     CHQKDSKRALWGGSQHETADVACFTCHKNHTNQDKVLARVTQTDVCLTCHKEQRTQLA
                     RPSHHPIPEGKMTCSDCHNVHGSAGPKLAKRDSTNNTCYTCHAEKRGPFVQNHEPVME
                     DCAICHNPHGSTVAAMLKTRPPMLCQQCHTPHVGGGVGALGGQTGVFPPAAAPGQLSS
                     AVTGSSGGKNVVNIWQGRSCTNCHTQVHGSNNPSATNPTPKFRFR"
     gene            6056..8260
                     /locus_tag="VGA59_11765"
     CDS             6056..8260
                     /locus_tag="VGA59_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HEX9720401.1"
                     /translation="MNSLFQPKNARLSLLALALLSAFGSVQAQIAPPVEGSVGVGLGL
                     VSGDRQDRLLFDQYNGLRPGSNAYGIFGAEYYRRDDAKGTAVDFQVSDLLTGNRELGL
                     RWKKQGDWKFSADYRELVRRDTQVPNTGLIGAGTTNPLAVVLPGGPGTGSDLDLKIKR
                     SNLGFAVSKVISSALQLDASVQTEKKEGSRLWGIGFNCPSPIAPGCRTTTGAELGWAV
                     LMMPEPINANHSQAEARLSYASGKLHLSGGYYGSFYRNDYGSLNPVVPDRLYNNVLGT
                     TLPLSTGFQPILNQAVALPPDNQAHHLDLTGSYALAPTTLLNFKLGYSRATQDQNFAA
                     AGFAAAPAGVSDLGGKMTTALAQVGFTARPLPKLSVSGNLRYEDRDDDTPLALYNVED
                     TSRYTNRQLPYRNVRGKLQAGYQFSSDYRGTLAATYQSIDRGAFTSSSAVAGITALRQ
                     ETEEAGLRAELRRRMSQDVSGAVSVESSRRDGSNWLRDNSGVGVTEVTTDANAVGPAF
                     TTGIFMPTLADRKRDKIKLQADWQPSEALSLQFMAEAGKDRFSSPSSYGLRDASMNSV
                     GMDLTYAVNDKWNLNGYVSRGRQQLNQARPEAAVIAFDNTATTLGVGFTGKPMGQLEV
                     GGTLAFMNDRSEFAQTLDTNAGLGSAALLAATGGLPDIVFRQTTLKLFGKYTLDKQSA
                     VRVEVGHQRSTWNDWAWNFNGTPFVYSDATIVNRQPKQRVSFIGVSYIYRWQ"
     gene            8561..8893
                     /locus_tag="VGA59_11770"
     CDS             8561..8893
                     /locus_tag="VGA59_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9720402.1"
                     /translation="MRATPLLAAAAAVFAFSLPAAAAPDAAAATKLLKDSGCTKCHSV
                     DKTKKGPAFKAVAAKYKGKAEGEAKITDVITKSPKVKMGDSEEEHAAVKTKDATQVKN
                     LVQWILAQ"
     gene            8931..9662
                     /gene="rquA"
                     /locus_tag="VGA59_11775"
     CDS             8931..9662
                     /gene="rquA"
                     /locus_tag="VGA59_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodoquinone biosynthesis methyltransferase
                     RquA"
                     /protein_id="HEX9720403.1"
                     /translation="MPPPPVADAVFPAPAPAPALKLPIPGYLEQVYWWAYVHPNAVRL
                     FEREWLVNLILFGNYGRLRDAALADLGETVHGKTLQVACAYGNITPRLASRLAPDASL
                     DVVDILPIQLLNLGKKLPADDRIALLQGDSSSLACADESYDQVLLFFLLHEQPEAVRR
                     ATLSEALRVVKPGGKIVIVDYHRPQAWHPLRLLMTGVFRKLEPYAMDLWRNEVEAFLP
                     RHDRPVSIAKKTYYGGLYQKLVLTR"
     gene            9693..>10415
                     /locus_tag="VGA59_11780"
     CDS             9693..>10415
                     /locus_tag="VGA59_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9720404.1"
                     /translation="MDVFKADVAIVGAGGAGLRAAIAVAEADPSLTIALISKVYPMRS
                     HTVAAEGGAAAVTQSHDSLEAHFHDTVAGGDWLCEQDVVEFFVGQAHEEMVQMEHWGC
                     PWSRTADGRANVRAFGGMKIERTWFAADKTGFHMLHTLFQTSIKYPSIKRFDEHFCAD
                     LLVDEGRVQGVVAIEIATGEFRMIQAKSVIIATGGAGRVFRENTNGGIVTGDGMALAF
                     RHGVPLRDMEFVQYHPTCMPGTG"
BASE COUNT         2142 a         3524 c         3175 g         1574 t
ORIGIN      
        1 gcagggtgcg ggcgtgccgg cggtgacggt gaacagcgcg cccaaggtga acttcacggt
       61 cttctcggac ggcgccatca agcagggcct ggccctctcg gacgtgagct tcatcatctc
      121 caagctcgtc cccggcagca acggcgaccc ggacaagtgg gtgagctaca cctaccgaac
      181 cgagtccacg gccagcggca ccgccaagac gggtcccggc ggcgttccgg tgctcgcgtc
      241 ggctctgcaa gccacgaccg acaccaagct gacgggtacc gcggcggcga cgcagctggt
      301 ctacaacccc gatggctact acacctacac cttctcgacc gacatcacgg atccgacgaa
      361 gacgcaaggc gtggtgttcg agccgaacct ggcccatcgc gtcgcgatcc agctcagcta
      421 cacgaatgcc gccggcacga cgattcgcgt caacccgtac ttcgacttca ccgtcggggc
      481 ggacggcaag gcggtggccg ctaccgcgcg caagatggtc gacgtcgcaa cctgcaacgg
      541 ctgccacgac gagctcaggc tgcacggcgg cgggcgcaag gacacgcagt tctgcgtgct
      601 gtgccacaac ccgagcacgg ttgacgccca gagcggcaac aacctgaacc tcgccacaat
      661 ggtccacaag atccactcgg gccgggaact ccatgaaaac ggcgagaatt acaccatctg
      721 gggcttcaac gccagcaagc acgattactc ggaagtgggc ttcccgcaac ccacgcgcaa
      781 ctgcgccgcg tgccacaccg gcagcaatcc cgccacaccg caaggcgaca actggaagag
      841 caagcccgcc aaggaagcct gcctgagctg tcaccagtcc ggcgacacca gcgcctggtt
      901 cgcgacccac gtgaccgccc tcaagctggg cgcctccgcg gccgccatcc cgaactcctc
      961 gtgcaattcc tgccacggcg ccaatagcac gtgggccccg gaaaaagcgc actgggtcca
     1021 ggaaatggcc aacgccgccc tttacaaggg cgtgatcgag agcgtgacgg tcaccaaggc
     1081 accgaccgcc gccgccgctg gaacgataag cgtcaagtac gccgttatca atccggcgac
     1141 cggcgctgcg tacgacctgg ccgaagggtg caccggcgcg gcgaccactg accaaaaagg
     1201 caatccgatc gccaactcct gcaataccaa ttaccggtgg gatgcgaacc tcgacccgac
     1261 gaagtggacc gctccgccga acaagttcgg taccttcacg ctgggcctcg ccaccaacac
     1321 gctcgctcaa accctcgacg atacgaccgg aacagccggc ggcgttctcc ccgcctaccg
     1381 cggggtcaag gatgccggca atcactacac gatggagatc acggtacctg ccgcctcgaa
     1441 aggcaatgca aggctgctgc tgctcggctc ggtcgcagaa cccaggctcg atccgatgac
     1501 acgcacggcc gccggtgcta tcccggccac gaagaatggc gacgtggcct acgtgccggt
     1561 gaagaacgac gtctacgagt tcaccgcgac aggaaacggt gcgcgggcaa ccagcacggc
     1621 gcggcgccag atcgtcagca atgacaactg caacagctgc cacggcatac ttggcctgcc
     1681 caccggtccg aatgaaccgg agcgtacagg ctttcacaag ggcgtgcgca ataactccga
     1741 agggtgcgtg aactgccaca acgccaacca ggcgggcggc tacacgctca tgaccgacgg
     1801 cagcaccggc ccgatcgcgg gtgacagcca actggcccag ccgaacacga gttcgttcct
     1861 gcacgagtcc tatcaggcca agcgattcat ccacggcatc cacggcggcg cgaagcgcac
     1921 atatccgttc acccactgcc tgaccacggg cggcgagtac aacaaggacg gcaccagcaa
     1981 gaccggcggc aagcccctgg gcgatgaaac ctgcgtgaat taccctgggg agacgcttaa
     2041 cttcaccgcc gaagtagcct acccggccca gctcggcaac tgctcgaact gccacgtgaa
     2101 tgactcctgg aagcaggaca ggagcgtgat cggatcggtg gtgttcaagc ccgtcggcgc
     2161 caccgcactg gattggctgg tgatctcgcc gaaggcggcg acctgcacgt cctgccacga
     2221 ctcgaaggcg gtgcagacgc acgttacggc acagggcggc gccagtttcg ggaccgtgac
     2281 acaggacggc cttctcaaca agggcgcggt attcgaggcc tgcacaggat gccacatacc
     2341 cggcagcccc attggcgtgg acgtcgtgca cggcttcaaa taggcaagat gttgcatacg
     2401 ggttgaaaga gcatccgtga caacgatgtt gtgaaaaaaa aagggccggc cggccggccg
     2461 gccctttatc atgtgtcgga ccgatccgaa cgaggagcac taacgtgatg aaatcttggg
     2521 ttcgagtgag cgctacagta ctgttcgctc tggctatcgc cggttgcggt ggtggcggtg
     2581 gtggcggggt tgaccccggt accggtggca cgggaacccc gacggggtcc gtaagcgaag
     2641 cgatcgccaa ggcggccgcg gtcccagcga acgatacctc gaccaactca tccgcgccgt
     2701 ttgcggtggt ccaggccgcc ggcgtgccgg ccgtcacagt tgccagccct cctgtagtca
     2761 acttctccgt cttttccgac ggcaaggtaa aaaccggcct ggagctttcc aacgtcagct
     2821 tcgcgatcgc caagctggtg cccggcacca atggcaacct cgaccaatgg gtcagctatg
     2881 tctaccgcac ggagacagcc gcggcaggcg tcggcccggg cggagctccc gttctggcca
     2941 aggcgacaca ggcgaccacc gacgccaagc cggctgccgc cgccgcgaca cagctggtct
     3001 ttaacgccga cggctactac acctacacct tcacgaccga catcaaggac ccggcgaaga
     3061 ccaacggtgt gacgttcgag ccgaaccgga cccaccgcgt cgccattcag ctgagctacg
     3121 tgaacgccgc cggtgagacc gagcgcgtca acccctattt cgatttcact ttcgatgcca
     3181 atggcaactc agtgcctgtc accgacccgg ccttgaccaa gaaaatgacg gacgtttcct
     3241 catgtaatgg ctgccacgag aagttggcgc tgcatggcgg cggtcgcgtc gatacgcagt
     3301 tctgcgtgat gtgccacaac cccggcacca ccgacgccaa cagcggcaat gtgctgacac
     3361 tatccacgat ggtgcacaag atccacgctg gcaggctgct gcactccaaa ctcagcgccg
     3421 gcggcgagga ttacgtcatc tggggctatc gcgatacccc gcacgattac tcggaagtcg
     3481 gcttccccca agacctgcgc aactgcacga agtgccatac cggcgccaac ccggccacgc
     3541 cccagggcga caactggaag accaaggcga gccgtgaagc ctgcctgacc tgccatgcga
     3601 acaaggaagg ctccgaatgg ttcagcttcc acaagatcct gaacaacgac caggacccca
     3661 acgccgccgc gacgctcatg cccaccagcg catgcgcggg ctgccacgca cccggaacct
     3721 cgattgcgcc cgaacgcgtc cactggaacc agaacgagga gaacgcggcc aagtacaaga
     3781 tgaacatcga agacgcggcg tacgacgcgg ccacccgcaa ggtgacggtg aaatacttcc
     3841 tgtccgaccc gaccaatggc aatgccgcct acaacctggt cacgccagag tgcacgggca
     3901 cgagcacgat cgcttgcagc aaccagaccc ggttcggcaa cctcaggttc tacctgccgc
     3961 accagaacat gatcggccag cccacgggga cgaccgaatt ctccgcccac aacaacggcg
     4021 gcagtagtgc caacgcattc gcctataagg gcaccaacga tggctccaac cattacaccg
     4081 tgcagatttc ggttccgccc gactccacgg tttcgatggc cttcggcacg gcgcgcgtcg
     4141 tcagcatcgg gcagatcaag gaacccaagc tgcaggtgaa gaccgtcagc gatccgaggc
     4201 cggaagtgac gccgcgcgaa ctcgtcaacg tggtcgtgca gcacacgttc aaggacgtgg
     4261 tcctgagcgg gacgctgaat ccccgccgtg cggtcgtctc caacgagaag tgcaacgtct
     4321 gccacggcgc gctcggcacc acgtcgggct ccaacacgct ggccaacgcc ttccatggcg
     4381 gcgcacgcaa cacggtggaa gcctgcgtga tctgccacga cccgaaccgg atgtcctcca
     4441 cgaagatgac caatggcctg gacctgaacg agtcctacca gttcaagcgc atgatccacg
     4501 gcatccacgg caactccaaa cgcacctatc cgtttaccca cggcaacaag gtgatcgacg
     4561 ccttcaacaa ggacggcacc tcgaagaccg gcggccttcc catgctggag gctaacctgg
     4621 cgatcggcaa cacgagcacc gtggagaact acgccgccga ggtcgcctgg ccaggcgtcg
     4681 ggctcaactg caatgcctgc cacgtcgaca actcgtacaa gctggaccgt ggcaccctgg
     4741 gtgctgtcgt gaccaagcct gccggcgtga ccgatccgat gcagtggctg gtgatttccc
     4801 cgaaggcggc cacctgcacg gcatgccatg attcgcccgc ggccatgggc cacgtgacga
     4861 gcttcggcgc cgctacgttc gccaaccgga cgcaggccga gtcgctgcag atccaggaaa
     4921 cctgcgcgga ctgccatgcc agcgggattg ggttcaaggc agtggatatc gtccacggac
     4981 aagagtaaga aaagcaacca ggggccggcg actggggcac ggaaccagtc gccggcaacc
     5041 cgctacaggg ccgaggggcc gaggagatcg aatgcgaatc aagcgagcgt tggcggccat
     5101 cgcgctgggg ctatttgggt tggtcgcaac ggtgggcgcg gccggcgtgg atgcggaggc
     5161 ttcggcggcc tgcgtgaaat gccacgacga ggaggacatg ccggacaaca gccgccatgc
     5221 gcacgctgtt gtcacggatt cccgcacccc gacctgcgtg acctgccatg gaccgagcac
     5281 cgctcaccgg gccaagcctt ccgacaccaa gcccgatcgc ctgttcggca ggaaagcgaa
     5341 cctgccgacg agcgaactca atggcgcctg cctgacctgc caccagaagg actcgaagcg
     5401 cgcgctgtgg ggcggaagcc agcatgaaac ggccgacgtc gcctgcttca cttgccacaa
     5461 gaaccacacc aaccaggaca aggtcctggc tcgggtgacc cagaccgacg tctgcctgac
     5521 ctgccacaag gagcagcgca cgcagctcgc ccgaccgtcg caccacccca tacctgaagg
     5581 gaaaatgacc tgttcggatt gccacaacgt gcatggatcg gcgggcccga aactcgccaa
     5641 gcgcgacagc accaataaca cttgctacac ctgccacgcg gagaagcgcg gcccgttcgt
     5701 gcagaaccat gagccggtca tggaggactg cgcgatctgc cataacccgc acggcagcac
     5761 cgtggcggcc atgctcaaga ccaggccgcc gatgctgtgc cagcagtgcc atacaccgca
     5821 cgtgggcggc ggggtcggcg ccttgggggg ccagaccggc gtcttcccgc cggccgccgc
     5881 acccggacag ctttcttccg ccgtcaccgg ctcgagcggc ggcaagaacg tcgtgaacat
     5941 ctggcagggc cgcagctgta ccaactgcca tacccaggtg cacggttcga acaacccgtc
     6001 ggcgaccaat ccgacgccga agttccgttt ccgctgagac cggagaatga ataatatgaa
     6061 ctccctcttc cagcccaaga acgcccgcct gtcgttgctg gcgctggcgc tgctctcggc
     6121 attcggttcg gtccaggccc agatcgctcc tcccgtcgaa ggatccgtgg gcgtcggcct
     6181 cggcctcgtc agcggcgatc gccaggaccg gctgctcttc gaccagtaca acggcttgcg
     6241 cccgggcagc aatgcctatg gaattttcgg cgccgaatac taccggcggg acgatgcgaa
     6301 gggtaccgcc gtcgacttcc aggtctccga tctcctgacg ggcaaccgcg aactgggact
     6361 gcgctggaag aaacaggggg actggaagtt ctccgccgat taccgcgaac tcgttcggcg
     6421 cgacacccag gtccccaaca ccggactgat aggcgccggg acaaccaacc ccctggcggt
     6481 ggtcttgccg ggcgggccgg gcacgggctc cgacctggat ctgaagatca aacgcagcaa
     6541 cctgggtttc gcggtctcca aggtgatcag cagtgccttg cagctggacg cgagcgtgca
     6601 gaccgaaaag aaggaaggtt cgcgcctgtg gggcatcggc ttcaactgtc cgtccccgat
     6661 cgcgcccggc tgccgcacca ccaccggcgc ggagctcgga tgggccgttt tgatgatgcc
     6721 ggagccgatc aacgccaatc acagccaggc cgaagcgagg ctgagctatg ccagcggcaa
     6781 gttgcacctg tccggcggct actatggctc gttctaccgc aatgactacg gcagcctgaa
     6841 cccggtggtt cccgatcgcc tttacaacaa cgtactgggc acgacgcttc ccctttcgac
     6901 cgggtttcag cccatcctca accaggcggt ggcgctgccc cccgacaacc aggcgcatca
     6961 cctggacctg accggcagct acgcgctggc gccgaccacg ctgctcaact tcaagctcgg
     7021 ttactcgcgg gccacgcagg accagaactt tgcggcggcc ggctttgccg cggcgccggc
     7081 cggtgtctcc gacctgggcg gcaagatgac gaccgccctc gcgcaggtcg gattcacggc
     7141 gcgtccgctg cccaagctgt cggtatccgg aaacctgcgc tacgaagaca gggacgacga
     7201 cacgccgctg gcgctctaca acgtggaaga cacgtcgcgc tacaccaacc gccagcttcc
     7261 ctaccggaat gtccgcggca aattgcaggc gggttaccag ttctccagcg actaccgcgg
     7321 cacgctggcc gcgacctacc agtccatcga ccgcggcgcg ttcacttcca gcagcgccgt
     7381 ggccggtatc accgccttgc gccaggagac cgaagaagcg ggcctgcggg ccgaattgcg
     7441 ccggcgcatg tcccaagacg tcagcggcgc cgtgagtgtg gaatcgagcc ggcgggacgg
     7501 ctcgaactgg ctgcgcgaca acagcggtgt cggtgtgacc gaggtaacga cggacgccaa
     7561 tgccgtgggc ccagccttca cgaccggcat cttcatgccg acgctggcgg accgcaagcg
     7621 cgacaagatc aagctgcagg ccgattggca gccgagcgaa gcgctgtcgc ttcagttcat
     7681 ggccgaagcg ggcaaggacc ggttctcctc ccccagcagc tacggcttgc gcgacgcctc
     7741 gatgaactcc gtgggcatgg acctcacgta cgcggtgaac gacaagtgga acctcaacgg
     7801 ttatgtgtcg cgcgggcgcc agcaactcaa ccaggcgcgg ccggaagcag ccgtcattgc
     7861 cttcgacaac acggccacga cgctcggggt cggcttcacc ggcaagccaa tgggccagct
     7921 cgaggtgggc ggcacgctcg ctttcatgaa cgatcgcagc gagtttgcgc agacactgga
     7981 cacgaacgcg gggctcggca gcgccgcgct tctggccgcg acgggcggac tgcccgacat
     8041 cgtgttccga cagacaaccc tgaaactgtt cggcaagtac acgctggaca agcagtccgc
     8101 ggtgcgggtc gaggtggggc atcagcgttc cacctggaac gattgggcgt ggaacttcaa
     8161 cggcacgccg tttgtgtact ccgacgccac gatcgtgaat cgccaaccga agcagcgagt
     8221 cagcttcata ggcgtgtcgt acatctaccg ctggcagtga caccgcgcgc aagcgtgacg
     8281 cttgcgcggc agtgacacga cgacaacatc acgacccgcg gacaccacgt gtcttatatc
     8341 aagacgctgg gtctctgggg cgccaacacc aaggccgata tcaaggccgt gaaactgccc
     8401 tgagaaacct tgccgtccgc tcggtcgttc aagacccata ggcataatct gaactgtggg
     8461 ggggcgttga cattcgtcaa tcccccctct actggctggc ggcaatatgc cctcatccaa
     8521 tagaaggcta tttcaccatt ttcttaacag gagcccaaac atgagagcca ctcccctgct
     8581 ggcagccgcc gctgccgtat tcgcattctc gctgccggcc gcagcggcac ccgatgccgc
     8641 cgccgccacc aagttgctga aggacagcgg gtgcacgaaa tgccattccg tcgacaagac
     8701 gaagaagggc cctgctttca aagcggtagc cgccaaatac aagggcaagg ccgaaggcga
     8761 agccaagatc accgacgtga tcaccaagag cccgaaggtc aagatgggcg acagcgagga
     8821 ggagcacgca gccgtcaaga ccaaggacgc cacccaggtc aagaacctgg tgcagtggat
     8881 cctcgcgcaa taagcgcgcc gacgacaagc agtcaccgag gtcgcaaccc atgccgccgc
     8941 cgcccgtcgc agacgccgtc tttcccgcgc ctgcgccggc tcccgccctg aagctgccga
     9001 ttccgggcta cctggagcag gtctactggt gggcgtacgt tcacccgaac gccgtacgct
     9061 tgttcgagcg cgagtggctg gtcaacctga tcctgttcgg caactacggg cggctcaggg
     9121 atgcggcact ggccgatctc ggcgaaaccg tgcatggcaa gacgctgcag gtcgcctgcg
     9181 cttacggcaa catcacgccc aggcttgcct cgcgcctcgc gcccgacgcg agcctggatg
     9241 tggtggacat cctgccgatc cagttgctga acctgggcaa gaagctgccg gccgatgacc
     9301 gcattgcgct gctgcagggc gactcgtcgt cgctcgcctg cgccgatgag agttatgacc
     9361 aggttctgct gttcttcctg ctgcacgaac aacccgaggc ggtacggcgc gccacgcttt
     9421 ccgaggcgct gcgcgtggtc aagccggggg gcaagatcgt gatcgtcgac taccatcggc
     9481 cccaggcgtg gcatccgctg cgcctgctga tgaccggggt gttccgcaag ctcgagcctt
     9541 atgcgatgga cctgtggcga aacgaggtcg aggctttcct gccgcgccat gaccggccgg
     9601 tctccatcgc caagaaaacg tattacggcg ggctctacca gaaactggtt ctgacccgtt
     9661 agccttcaac ccaagaaaga agaggccacc agatggatgt cttcaaggcc gatgtcgcga
     9721 tcgtcggcgc cggaggggcg ggcctgcgcg ccgcaatcgc ggtggccgag gccgacccct
     9781 ccctgaccat cgcgctcatc tccaaggtct acccgatgcg cagccacacg gtggccgctg
     9841 aaggaggcgc cgcggctgtc acgcagtcgc acgacagcct ggaggcccat ttccacgaca
     9901 ccgtggccgg cggggactgg ctgtgcgagc aggacgtggt cgagttcttc gtgggccagg
     9961 cccatgaaga gatggtgcag atggagcact ggggctgccc ctggagccgc acggccgacg
    10021 gccgcgccaa tgtgcgcgcc ttcggcggca tgaagatcga gcgcacgtgg ttcgccgccg
    10081 acaagaccgg cttccacatg ctgcatacgc tgttccagac gtcgatcaag tacccgtcga
    10141 tcaagcgctt cgacgagcac ttctgcgccg acctgcttgt ggacgaaggg cgagtgcaag
    10201 gcgtcgtggc gatcgaaatt gccaccggtg aattccgcat gatccaggcc aagtcggtga
    10261 tcatcgccac cgggggcgcc ggccgcgtgt tccgcgagaa caccaacggc ggcatcgtca
    10321 ccggcgacgg catggcactt gccttccgtc atggcgtgcc gctgcgcgac atggagttcg
    10381 tgcaatacca ccccacctgc atgccgggca ccggc
//
LOCUS       DASWIY010000329         3822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_957705, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000329 DASWIY010000000
VERSION     DASWIY010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957705"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VGA59_11785"
     CDS             complement(<1..466)
                     /locus_tag="VGA59_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9720405.1"
                     /translation="MTTRRLVLSRSAAIVGAASTGLLLPEIVRAQSNKVRVGFMLPYT
                     GTYAQLGVAIENGVRMAIDEKGGKLGGREIEWFKVDDESEPSKGVENASRLVQRDKVD
                     VLIGTVHSGVQMGIQKVARDSGVLCLIPNAGVHAATRALCAPNVFRTSFTNSQ"
     gene            complement(576..2144)
                     /locus_tag="VGA59_11790"
     CDS             complement(576..2144)
                     /locus_tag="VGA59_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX9720406.1"
                     /translation="MHLSRADHSTSPPRVEIPRDYNAAHDLLARNAARADKAAFVDAS
                     SGAQLTYGELTDQAHRFANALRAGGFSPETRVLLAMLDTLEWPVVFLGCILAGVVPIA
                     ANTLLTTKDFDFMLRDSRARALFVSKPLLPTFEPLIGSIGTLKTLVVAGDSAPNSVAA
                     MIASGAAALHVESTCADDACFWLYSSGSTGTPKGTVHLHSHLVQTAELYARAVLGIRE
                     QDVVFSAAKLFFAYGLGNSLTFPMSVGATAVLLPSRPTPADVFGVLTRFRPTIFYGVP
                     TLYAALLADAARPKREAVNLRVCTSAGEALPADIGKRWTEAYGVEILDGIGSTEMLHI
                     FLSNRPGEVRYGTTGKAVPGYELRIVGDDGRECGVGEIGELQINGPSAAIMYWNNRAK
                     TKATFAGEWTRSGDKYTLSADGYYTYGGRSDDMLKVGGIYVSPFEVEACLTTHAAVLE
                     AAVIGVADTDELVKPKAYVVLKPGQVATAEELQLHVKSLLAPYKYPRWIEFVAELPKT
                     ATGKIQRFKLRMSA"
     gene            2260..3174
                     /locus_tag="VGA59_11795"
     CDS             2260..3174
                     /locus_tag="VGA59_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9720407.1"
                     /translation="MQLEIDHDAKATMAELGSRVRGWRARRGMTRKQLAADSGLSERF
                     LADVESGRGNVSINSLEAVARALNITILELFQDAPRPAMGRVQAVLAQLDDAELDQAY
                     TLLAVNFGRADQNGRDKRIALIGLRGAGKTTLGAQLAAQRGVPFVELDREIEREAGTS
                     MNEILLLHGQAGYRRYERRALFRIAEDHADGVVVTTGGSIVSERETFDLLQSRFYCVW
                     LKANPEEHMSRVVAQGDLRPFHSTNGDDGHFAQGAMGEAMEDIRRILASREALYARAD
                     AVVDTAARSVKQSLKDLERAVPPMSVRP"
     gene            3347..3423
                     /locus_tag="VGA59_11800"
     tRNA            3347..3423
                     /locus_tag="VGA59_11800"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3381..3383,aa:Pro,seq:tgg)
BASE COUNT          706 a         1197 c         1224 g          695 t
ORIGIN      
        1 gctgggagtt ggtgaacgac gtgcgaaaga cattcggggc gcacagggcg cgagtggctg
       61 catgcacacc ggcattgggg atcaggcaca gcacgccgct gtcgcgcgcg actttctgga
      121 tacccatctg cacgcccgag tgcaccgtgc ctatcagcac gtccactttg tcgcgctgca
      181 ccagccggct ggcgttttcc acacctttcg aaggctcgga ctcgtcgtca accttgaacc
      241 attcgatctc gcggccgccc agtttgccgc ctttttcgtc gatggccatg cgcacgccgt
      301 tttcaatggc cacgccaagc tgggcgtagg tgcctgtgta aggcagcatg aaacccacgc
      361 gcaccttgtt ggactgtgcg cgaacgattt caggcagcag caggcccgtg gatgcggcgc
      421 cgacgatggc ggcgctgcgg gaaaggacta ggcggcgggt ggtcataaca aaagtctcct
      481 cacgatatgg gttctaaaca attcttaccg gacacagcgc ggctgcatac catgattacc
      541 cgcattcatc gcggtgccgg tgcgcgatcc atggctcatg cggacatgcg cagcttgaag
      601 cgctggatct tgccggtggc ggtcttcggc aactccgcga cgaactcgat ccagcgcggg
      661 tatttgtagg gagctagcaa gctcttcacg tgcagctgca attcttcagc cgttgcgacc
      721 tggcccggct tgagaaccac ataggctttc ggtttgacga gttcatcggt gtcggccaca
      781 ccgatcacgg cggcttcgag caccgccgca tgcgtggtga ggcaagcctc gacctcgaag
      841 ggcgaaacgt agatcccgcc caccttgagc atgtcgtcgc tgcgcccacc gtaggtgtag
      901 tagccgtccg cgctcagggt gtacttgtcg ccgctgcggg tccactcacc cgcgaacgtc
      961 gccttggtct tggcgcggtt gttccagtac atgatggccg cactcgggcc gttgatctgc
     1021 agctcaccga tctcgcccac accgcactcg cgtccgtcgt cgccgacgat cctcagttca
     1081 tagccaggca cggccttgcc ggtcgtgccg tagcgaacct cgcccggccg attggacagg
     1141 aagatgtgca gcatttccgt ggagccgata ccgtcgagga tttccacccc gtaggcttcg
     1201 gtccagcgtt tgccgatgtc ggccggcagg gcctcgccgg cgctggtgca tacgcgcagg
     1261 ttgaccgctt cgcgcttcgg ccgggccgcg tcggccagca aggcggcata aagcgtcggc
     1321 acgccataga agatggtggg ccggaacctg gtcagcacgc cgaacacatc ggcgggcgtt
     1381 ggtcgggagg gcaggagcac ggcggtcgcg ccgacgctca tggggaaagt cagcgaattg
     1441 cccaggccat aggcgaagaa caacttggcg gccgaaaaga cgacgtcctg ctcacggatg
     1501 ccgagcacgg cccgcgcgta cagttccgcc gtttgcacca ggtggctgtg caggtggacg
     1561 gtgcccttgg gcgtgccggt cgagccgctg gaatagagcc agaagcaggc atcgtcggcg
     1621 caggtgctct cgacatggag cgccgcggcg cccgacgcga tcatggccgc cactgaattg
     1681 ggcgccgagt ctcccgccac gaccaaggtc ttgagcgtgc cgatgctgcc gatcaaaggc
     1741 tcgaacgtcg gcagcaaggg cttggagacg aacaacgccc gtgcccgcga gtcgcgcagc
     1801 atgaagtcga aatccttggt ggtcagcagc gtattggccg cgatcggcac cacgccggcc
     1861 aggatgcaac ccaggaaaac caccggccat tcgagcgtgt cgagcatggc gagcagcacc
     1921 cgcgtctcgg gcgaaaagcc gccggcccgc agcgcgttgg cgaatcggtg ggcctggtcc
     1981 gtcaattccc cgtaagtcag ctgggcgccg ctcgaggcgt cgacgaaggc ggccttgtcg
     2041 gcgcgcgcgg cattgcgcgc cagcaggtcg tgagctgcgt tgtagtcgcg cgggatttca
     2101 acccgcggcg ggctggtgct gtgatcggcg cgactgaggt gcatcaattc gtctcctcgc
     2161 gtattgtcca tgtgcaataa actgcttgac aggtcattaa taggaagcat acttcaatct
     2221 tgatctagcg caaacgttat tcagaaaaac ccgctctcta tgcagttaga gattgaccat
     2281 gacgccaagg ctactatggc cgagctgggt tcgcgggtgc gcggctggcg ggcccggcgt
     2341 ggcatgaccc gtaagcagct ggcggccgat tcgggcttgt ccgagcgctt tcttgccgac
     2401 gtcgaaagcg gcaggggcaa cgtttccatc aattcgctgg aagcggtggc gcgggccctg
     2461 aacatcacca ttctcgagct attccaggat gcgccgcgtc cggcaatggg ccgtgtgcaa
     2521 gccgtactgg cgcagctgga tgatgccgag ctggaccagg cctatacgct gctggctgtc
     2581 aactttggcc gggcggatca gaacggccgc gataagcgca tagcgctgat cggcctgcgc
     2641 ggcgcgggga aaacgaccct cggggcccag ctcgcggcgc agcgtggcgt gccgttcgtc
     2701 gaactcgacc gtgagatcga gcgcgaggcc ggtaccagca tgaacgagat attgctgctt
     2761 catggacagg ccggctaccg gcggtacgag cgccgcgcgc tattccggat tgccgaggac
     2821 catgccgacg gcgtggtcgt gaccaccggc ggcagcatcg tgagcgagcg cgagaccttc
     2881 gacctgctgc aaagccgttt ctattgcgtc tggctcaagg ccaatcccga agagcacatg
     2941 agccgcgtgg tcgcgcaggg cgacctgcgg ccgttccatt caaccaacgg cgacgatggc
     3001 catttcgccc agggcgccat gggggaggcg atggaagaca tccggcgcat tctggccagc
     3061 cgcgaagcgc tgtacgcacg tgcggatgcg gttgttgaca ccgccgcgcg ctcggtcaag
     3121 caatccctca aggacctcga acgcgcggtt ccgccaatgt ccgttcgccc ctagactgcc
     3181 gatcccttca gcgcaaccag ggcgaatcgc ccctcacgga gacgaagtca atgaatgcga
     3241 tggccggcaa gaaggccaag ttcgactaaa agcgaatctg aaggccggga tcgaatacga
     3301 acatgtcaag cgttgaaatc ggccgggcca gctagaatgc cggcttcgga gcgtggcgca
     3361 gtctggtagc gcatctggtt tgggaccaga gggtcgtagg ttcgaatcct atcgctccga
     3421 ccattcaagc ccatgatttt catgggcttt cctgttggcc ggttgaacga gacactcccc
     3481 ggccgttcga ggtgccgcac gccgtgaaat agctcggccg tagcgtggcg ccccccctgc
     3541 cgcaggcaag cgcagggggt ttttctttca gcgccgcacg ccaagccatt actggctata
     3601 aagcgatctc acacgggctg cacatgcccg tggtccggtt tcttgccgcc ccgcgttcaa
     3661 aaggagacat tgtgaagatc cactatcacc ccgcttccac caccagccgc cccctgatgc
     3721 tgttcgccgc cgagcagggg ttggacgccg aattcgtgct ggtcgacatc ttcacgggcg
     3781 aacacgtgcg cgagccctac gtccagctca accccaacca gc
//
LOCUS       DASWIY010000330         3353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2083743, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000330 DASWIY010000000
VERSION     DASWIY010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083743"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VGA59_11805"
     CDS             <1..1004
                     /locus_tag="VGA59_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9720408.1"
                     /translation="MQGAWGAKDIFNEMAEQYVKRVNDMAGGRLRIDYLVAGAVVKAF
                     EVKDATSKGVLDAAHSVPVYWYGKHKAASLFGTGPVFGNDAHHTIAWFYEGGGQALYH
                     ELVQKTLGLNVVGFLNFPMPTQPFGWFKNPIKNAAGLKGLKYRTVGLAADLFQAMGAK
                     VTQLPGGEIVPALERGVIDAFEFNNPTSDMRFGAQDVIKNYMMGSYHQASESFEIIFN
                     KAKFDALPADLRAILRTGVEAVNSANFWFAMENYSRDLQVLKDKHKVNVLRTPDSIML
                     EQLKAWDTLVAELSKDPISKKIIDSQRAWYQRVVYYEQFNAANYKLAYEHVYKTKLF"
     gene            1089..1691
                     /locus_tag="VGA59_11810"
     CDS             1089..1691
                     /locus_tag="VGA59_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9720409.1"
                     /translation="MVKRFIYGVDQLSKTIGHAFAWCIVILTLGTSYEVFVRYALNNP
                     TSWAFDFSYTMYGAMFFMAGAYTLSRGAHVRADMFYRLWQPRTQAAIELTLYILFFFP
                     GILALVISGWSYGFDSMSIREVSVNSPAGVPIWPLKMLIPLGAGLLALQGVAEVLRCI
                     VCLRENRWSPRLHDVEELEKQLIEQHARAAMQETQQGAAR"
     gene            1688..3034
                     /locus_tag="VGA59_11815"
     CDS             1688..3034
                     /locus_tag="VGA59_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9720410.1"
                     /translation="MSGPVIALIMLGILVLTIMIGFPVAFTLMALGVGFGYYAYFQAG
                     QAFFDNRVFYLLTQNTFTVLNNDVLVAVPLFLFMGYMIERSGILDSLFFSIQVALRRV
                     PGSLAIATLITCALFATATGIVGAVVTLMGLLAFPAMLKAGYDVRLASGVVCAGGCLG
                     IMIPPSILLIVYGAMAGLSVVQLYAAAIFPGLLLAGMYIVFVTILAIAKPKLAPRLPK
                     EQTDVPTLQVVAMLAKSFVPLAGMVMCVLGAIMFGLATPTEAAAVGALGSVMLAAAYR
                     QFTWGRLKESVYLTARTSAMVCFLFVGSATFAGVFSYLGGEGIIKEFVLDLQLNPVTF
                     LIISQFIIFLLGWPLEWTEIIIIFVPIFLPLLPHFQIDPLLFGILVALNLQTAFLSPP
                     VAMAAYYLKGIAPPQVLLTEIFWGCLPFLFIVVVSMLVVYVWPGISLWLPRAIYGN"
     gene            3039..>3353
                     /locus_tag="VGA59_11820"
     CDS             3039..>3353
                     /locus_tag="VGA59_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9720411.1"
                     /translation="MDTSRLNWLSAADMARAVRDGAISAGELAEACLARVREVDAEVQ
                     AWAYLDPEHVLRQAHVLDDQRRHGMAAGPLHGVPVGIKDIIDTADMPTEDGTVLHAGR
                     MPD"
BASE COUNT          566 a         1018 c         1094 g          675 t
ORIGIN      
        1 agatgcaggg cgcatggggc gcgaaagaca tctttaacga gatggccgag cagtacgtca
       61 agcgcgtcaa cgatatggct ggcgggcgcc tgcgcatcga ctacctggtc gccggcgcgg
      121 tggtgaaggc gttcgaggtg aaggacgcca ccagcaaggg tgtgctcgac gcagcccatt
      181 ccgtgcccgt gtactggtac ggcaagcata aggcggcgtc gctcttcggc acgggaccgg
      241 tgttcggcaa cgacgcgcac cacacgattg catggttcta cgagggcggc gggcaggcgc
      301 tctaccatga gctggtgcag aagaccctgg gactcaacgt ggtgggcttc ctgaacttcc
      361 cgatgccgac ccagccgttc ggctggttca agaacccgat caagaatgcg gcggggctga
      421 aaggcctcaa gtaccgcacc gtgggcctcg cggccgacct gttccaggcg atgggcgcga
      481 aagtgacgca gctgccgggc ggcgagatcg tgccggccct cgagaggggc gtgatcgacg
      541 ccttcgagtt caacaacccg acctccgaca tgcgcttcgg cgcgcaggac gtgatcaaga
      601 actacatgat gggcagctac caccaggcgt ccgaatcctt cgagatcatt ttcaacaagg
      661 cgaagttcga cgcgctgcct gctgatctca gggcgatcct caggaccggc gtcgaggcgg
      721 tgaactcggc gaacttctgg ttcgcgatgg agaactactc gcgcgatctg caggtcctga
      781 aagacaagca caaggtcaac gtgctccgca cgccggacag catcatgctc gagcagctca
      841 aggcctggga cacgctggtg gcggagctga gcaaggaccc gatcagcaag aagatcatcg
      901 attcgcagag agcgtggtac cagcgcgtcg tttactacga gcagttcaac gccgccaact
      961 ataagctcgc ctacgagcac gtgtacaaga ccaagttgtt ctgattgccc cgaagctcca
     1021 cagtttttcg gatcatgggg cggcaccgcg aggtaccgcc ccatttttct gcaatttccg
     1081 ggtaacccat ggtcaagcga ttcatctacg gcgtcgacca gctgagcaag acgatcggcc
     1141 acgccttcgc gtggtgcatc gtgattctca cgctgggcac ctcgtacgaa gtgttcgtgc
     1201 gttacgcact gaacaatccc acgagctggg cgttcgactt cagctacacg atgtacggcg
     1261 ccatgttttt catggccggg gcctacacgc tttcgcgcgg cgcgcacgtg cgggccgaca
     1321 tgttctatcg cctgtggcag ccgcgcaccc aggcggcgat cgagttgacg ctctacatcc
     1381 tgttcttctt tccgggtatc cttgccctcg tgatttcggg ctggagctac ggctttgact
     1441 cgatgagcat ccgcgaggtg agcgtcaaca gtccggccgg cgttcccatc tggccgctga
     1501 agatgctgat ccccctcggc gcggggctgc tcgcgctgca gggcgtagcc gaggtcctgc
     1561 gctgcatcgt ctgcctccgc gagaaccgct ggtcgccgcg cctgcatgac gtcgaagagc
     1621 tggagaagca gctgatcgag cagcatgcaa gagccgcgat gcaggagacg cagcagggag
     1681 cggcgcgatg agcggtccgg tgatcgccct gatcatgctg gggatcctgg tcctgacgat
     1741 catgatcgga tttccggtcg ccttcacgct gatggcgctg ggggtgggat tcggttatta
     1801 cgcgtatttc caggcgggcc aggcgttttt cgacaaccgg gtgttctacc tgctcacgca
     1861 gaacaccttc accgtgctga acaacgacgt gctggtggcg gttccgctgt tcctgttcat
     1921 gggctacatg atcgagcgct ccggcattct ggacagcctg tttttcagca tacaggtcgc
     1981 gctcaggcgc gttcccgggt cgctggccat cgcgacgctg atcacctgcg cgctgttcgc
     2041 cacggcgacg gggatagtcg gcgcggtggt gacgctgatg ggactgctcg cttttccggc
     2101 gatgctcaag gccggctacg atgtccgcct cgcctccggc gtcgtgtgcg ccggcggctg
     2161 cctgggcatc atgatcccgc cgtcgatcct gctgatcgtg tacggcgcca tggccggctt
     2221 gtcggtcgtc cagttgtacg cggcggcgat ctttccgggc ctgctgctcg cggggatgta
     2281 catcgtgttc gtcacgatcc tggcgatcgc caagccgaag cttgctccgc gcctgcccaa
     2341 ggagcagacc gacgtcccga cgttgcaggt ggtcgcgatg ctggccaaat cgttcgtgcc
     2401 gcttgcgggc atggtgatgt gtgtgctggg agcgatcatg ttcggcctgg ccacgccgac
     2461 cgaggccgcg gcagttggcg cccttggctc ggtaatgctg gcggcggctt accgccagtt
     2521 cacgtggggg cggctcaagg aatcggtgta cctgaccgcg cgtacgtccg cgatggtgtg
     2581 ttttcttttc gtcggctcgg cgacctttgc cggggtgttc agctatctcg gtggcgaggg
     2641 cattatcaag gagttcgtgc ttgacctcca gctcaacccg gtgacgttcc tcatcatttc
     2701 acagttcatc attttcctgc tgggctggcc gctcgagtgg accgagatca tcatcatctt
     2761 cgtgccgatc ttcctgccgc tgttgccgca cttccagatc gatccgctgc tcttcggcat
     2821 cctggtggcg ctgaatctgc agacggcgtt tctgtcaccg ccggtggcga tggccgccta
     2881 ctacctcaag ggcatcgcgc cgccgcaggt gctgctcacg gaaatcttct ggggctgcct
     2941 gccgttcctg ttcatcgtgg tggtatcgat gctggtggtg tacgtgtggc ccgggatttc
     3001 gctctggctg ccgcgcgcga tctacggcaa ctgacaggat ggacacctcg cggcttaact
     3061 ggctctcggc ggcggacatg gcgcgcgcgg tgcgcgacgg cgcaatcagc gcaggggaac
     3121 tggcggaggc gtgtcttgcg agggtgcggg aagtcgatgc cgaggtacag gcctgggcct
     3181 acctggatcc ggaacacgtg ctcagacagg cacacgtgct cgacgaccaa cgccggcatg
     3241 gcatggcggc cggcccgctg cacggggtgc cggtcgggat aaaggacatc attgacaccg
     3301 ccgacatgcc gaccgaagac ggcacggtac tccacgcggg acggatgccg gat
//
LOCUS       DASWIY010000331         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_215731, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000331 DASWIY010000000
VERSION     DASWIY010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215731"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1545
                     /locus_tag="VGA59_11825"
     CDS             <1..>1545
                     /locus_tag="VGA59_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720412.1"
                     /translation="QFDDVNVVASLSGPDAFAAIGAGVLQEPTVESPLPGGIAAVVRF
                     FFNLPAWFQITGLVIGALVAAGVLFVLWRRRQAIIAWATTRPRHVKLWLGGAAAALLL
                     FGAAFGGLSWNYMQHDNGFCTGCHVMGPSYARFTQSEHSTLNCHDCHQQSMFASMRQL
                     YLWVAERPAEIGPHAKVANTVCASCHVTGEREVWQRIASTAGHRTHLESDNPALRDVQ
                     CVTCHGVEVHHFAPLDRTCAQAGCHEMSQIALGRMSGQTTLHCTTCHRFTAEVPTLAT
                     RDSAAGTLTPGGRQCFSCHEMRQRLAGMDMDPGRDPHRGSCGMCHNPHRQQQPAAARA
                     TCTQSACHPDWRSEPFHLGVSHRRAVQQCTTCHLPHQAAVDASDCAGCHRAVGEREPG
                     RRALPQPFDTTRALRRTSWVGPHEEPRGKGDASPPREDPGDYVARFPLPAQPDTFSHD
                     RHKQLSCITCHAVAQGGGRLTFEAPRGCQICHHQAPATSTCATCHTPAELDSARVVRA
                     RVIVAG"
BASE COUNT          240 a          550 c          515 g          240 t
ORIGIN      
        1 ggcagtttga cgatgtgaac gtggtcgcct cgctttccgg ccccgacgcc ttcgcggcga
       61 tcggggccgg cgttctgcaa gaaccgacgg tcgagtcgcc tcttccaggg ggaatcgcgg
      121 ccgtggtccg gttcttcttc aacctcccag cctggttcca gatcacgggc ctggtcattg
      181 gagcgctggt ggccgccggt gtcctgttcg ttctctggcg ccggcgccag gccatcatcg
      241 cctgggcaac cacacggccg cggcatgtga agctgtggct cggcggcgcc gccgccgcac
      301 tgttgctatt cggggcggcg ttcggaggcc tgagctggaa ctacatgcag cacgacaacg
      361 gcttctgcac gggttgtcac gtcatggggc cgtcgtatgc ccggttcacg cagagcgagc
      421 acagcacgct caactgtcac gactgccacc agcagtcgat gttcgcgagc atgcggcagc
      481 tgtacctctg ggtggcggag cggccggcgg agatcgggcc ccacgccaag gtggcaaata
      541 cggtgtgcgc cagttgtcac gtgaccggcg aacgcgaggt ctggcagcgc atcgcgtcca
      601 cggccggcca ccgcacgcac ctcgagtccg ataacccagc gctgcgcgac gtccagtgcg
      661 tcacctgtca cggtgtggaa gtgcatcact tcgcaccctt ggatcgcacc tgcgcacagg
      721 caggctgtca cgaaatgagc cagatcgccc tgggccggat gagcggtcag acgacgctgc
      781 actgcactac gtgtcaccgc ttcactgccg aagtgcccac gctggccacc cgcgactcgg
      841 ccgcgggcac gctcacgccg ggagggcgcc agtgcttctc ctgccacgag atgcgccagc
      901 ggcttgcggg catggacatg gacccgggca gggatccgca ccgcggtagc tgcggcatgt
      961 gccacaaccc tcaccggcag cagcagccgg cagcagcccg ggcgacgtgc acccagtcgg
     1021 cgtgccaccc cgattggcgg agcgagccat tccacctggg ggtgagccat cgccgcgcgg
     1081 tccagcagtg taccacgtgc catctgccgc atcaggccgc ggtggatgcg agtgactgcg
     1141 ccggctgcca ccgggcggta ggggaacgcg agccgggccg gcgcgcgctg ccccagccat
     1201 tcgacacgac gcgcgccctc cgccggacct cctgggttgg cccccatgag gaaccccgtg
     1261 gcaagggaga cgcgtcgccc ccgagggaag accccggcga ctacgttgcc cgtttcccgc
     1321 tgcccgctca acctgacacc ttctcccacg accgccacaa gcagctctcc tgcatcacct
     1381 gccacgctgt ggctcaaggg ggtggacgcc tcaccttcga ggcgccgcgc ggttgccaga
     1441 tctgccacca ccaggcgccg gcgacgagca cctgtgccac gtgtcacaca ccggccgaac
     1501 tcgattcggc gcgcgtggtg cgggcgaggg tcattgtggc gggcc
//
LOCUS       DASWIY010000332         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2813671, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000332 DASWIY010000000
VERSION     DASWIY010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2813671"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VGA59_11830"
     CDS             <1..737
                     /locus_tag="VGA59_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9720413.1"
                     /translation="YKALGQRLEQIGASGAQVLGLADTHASNEELHLFKRLLADGFGS
                     DKVYFPHRPGRQQEQPPRYLTDTFIFTLITTDKSPNTAGALKAGLSGDEDDKRLKAAL
                     KTPPKVVLLLGTPFANDLAVREAVAKAELIVAIATHHSAWVELADVVLPGHTVAEREG
                     SYLNKDARLQRIQPAIEAPEGTQPQLQILGALLELRGKGQPHDSARAVFDAMAAQDGP
                     FKGLSWDRIGALGLSLAGAQPAQASA"
     gene            804..1346
                     /locus_tag="VGA59_11835"
     CDS             804..1346
                     /locus_tag="VGA59_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX9720414.1"
                     /translation="MAIQQIFFYIFAVMAVGGALSVLLFRSPVYCALALVGTFGFLAA
                     IFILLNNEFVAAIQILIYAGAIMVLFLFVIMLLNLRTVTPFSLKWSLPKVLGVAVSIG
                     ILAQMIGVFTSPAARLGPVDVYSPERIANEGAIQIIGQILFTDYVLPFEVISVLLMVA
                     VIGAVIIAKRHLPEGGKEGE"
     gene            1343..1645
                     /gene="nuoK"
                     /locus_tag="VGA59_11840"
     CDS             1343..1645
                     /gene="nuoK"
                     /locus_tag="VGA59_11840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX9720415.1"
                     /translation="MIPVSHVLSLSAILFTIGVTGVIIRRNAIVVFMCVELMLNAVNL
                     GFIGFAYGMHSMVGVMFVFFVLIVAAAEAVIGLSIMLAIFRNKATLNVDEINLLRW"
BASE COUNT          303 a          580 c          602 g          315 t
ORIGIN      
        1 tctacaaggc cctggggcag cgcctggagc agatcggcgc cagcggcgcg caggtgctgg
       61 ggctggcgga tacccatgcc agcaacgagg agctgcacct gttcaagcgg ctgctggccg
      121 acggtttcgg cagcgacaag gtgtacttcc cccaccgccc ggggcggcag caggaacagc
      181 cgccgcgcta cctgaccgac acgttcatct tcacgctgat cacgacggac aagagcccca
      241 acaccgcggg cgcgctcaag gccggtctga gcggagacga ggacgacaag cggctcaagg
      301 ccgccctcaa gaccccgccc aaggtggtgc tgctgctggg cacgcccttc gccaacgacc
      361 tggccgtgcg cgaggcggtc gccaaggccg agctgatcgt ggccatcgcc acgcaccaca
      421 gcgcctgggt ggagctggcg gatgtggtgc tgccgggcca caccgtggcc gagcgcgagg
      481 gcagctacct caacaaggac gcgcgcctgc agcgcatcca gccggcgatc gaagcgccgg
      541 aaggcacgca gccgcagctg cagatcctgg gcgcgctgct ggagctgcgc ggcaaggggc
      601 agccgcacga ctcggcgcgg gccgtgttcg acgccatggc tgcgcaggac ggaccgttca
      661 agggcctgag ctgggaccgc atcggcgcgc tggggctgtc cctggccggc gcgcaaccgg
      721 cgcaggccag cgcctgaacc gcttgcaagg gagcatgcgc gtcatccccg ccgcggcggg
      781 gcaatgctag cgacgcagac gagatggcaa tccagcagat cttcttctac atcttcgccg
      841 tgatggccgt gggcggggcg ctgtccgtgc tgctgttccg cagcccggtc tattgcgcgc
      901 tggcgctggt gggcacgttc ggcttcctgg cggcgatctt catcctgctc aacaacgagt
      961 tcgtggcggc gatccagatc ctcatctacg ccggcgcgat catggtgctg ttcctgttcg
     1021 tgatcatgct gctcaacctg cgcacggtga cgccgttcag cctgaaatgg tcgctgccca
     1081 aggtgctggg cgtggcggtg tccatcggca tcctggcgca gatgatcggg gtgttcacca
     1141 gcccggcggc gcggctgggg ccggtggacg tgtactcgcc ggagcgcatc gcgaacgagg
     1201 gcgcgatcca gatcatcggg cagatcctgt tcacggacta cgtgctgccc ttcgaggtga
     1261 tctccgtgct gctgatggtg gccgtgatcg gcgccgtgat catcgccaag cgccacctgc
     1321 cggaaggcgg caaggagggc gaatgatccc ggtctcccac gtgctctcgc tgagcgcgat
     1381 cctgttcacc atcggcgtga cgggcgtgat catccgccgc aacgccatcg tcgtgttcat
     1441 gtgcgtggag ctgatgctca acgcggtgaa cctgggcttc atcggcttcg cctacggcat
     1501 gcactccatg gtgggcgtga tgttcgtgtt cttcgtgctc atcgtggccg cggcggaagc
     1561 ggtgatcggg ctgtcgatca tgctggccat cttccgcaac aaggccacgc tcaacgtgga
     1621 cgagatcaac ctgctgcgct ggtgaataac aacatcatcc acagattaca cagattacgt
     1681 tttctgcgga ggtcgcagat tggtcactag atcctaacct ctaactgccc acttatcgta
     1741 aacaatcagg atcgtcgcac gacgtcggcc gggcatgcgc cgtaatctgt ggaaatctgt
//
LOCUS       DASWIY010000333        10574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_960068, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000333 DASWIY010000000
VERSION     DASWIY010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960068"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VGA59_11845"
     CDS             <1..651
                     /locus_tag="VGA59_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX9720416.1"
                     /translation="MRLLHIDSSPLAGNSVSRQLTERIVAQWRTTHPGTVVEYLDLAA
                     DAPSHLSIDSLGFRAGPDAKDLSEVQRRENAISERLVSQFLAADVIVVGAPMYNFSIP
                     SQLKAWIDRVAQAGRTFTYTEKGPKGLAAGKTVIVASTRGGIYSSNPAMAGLDHQESY
                     LQTVFGFFGIADVQFVRAEGVAMGDAAKAQALSAADVVIKSLSAAPANEPEAALAA"
     gene            819..1091
                     /locus_tag="VGA59_11850"
     CDS             819..1091
                     /locus_tag="VGA59_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEX9720417.1"
                     /translation="MARTVQCIKLGKEAEGLDFPPYPGELGKRLYENVSKQAWADWLK
                     HQTMLVNENRLNLADQRARQYLARQMENHFFGGGADVAQGYVPPSE"
     gene            1108..2958
                     /gene="mnmC"
                     /locus_tag="VGA59_11855"
     CDS             1108..2958
                     /gene="mnmC"
                     /locus_tag="VGA59_11855"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEX9720418.1"
                     /translation="MNEPVEWLPDGTPRSPRFDDIYRSSTGGLEQARHVFLRGCGLPD
                     AWAGRPQWRILETGFGLGLNFLAAWRAWKDDPQRPRMLHFVSSEAWPVCAADLLRSAA
                     PHPELVPLATALAEQWFGLVGGMHRMAFEEGLVLLTLFVGDVKAALRQQPFNADSIFL
                     DGFDPQGNAAMWELPTLKAVARHCRRGTRLATWTVAGEVRRGLTQCGFVVEKAQGLAP
                     KRHCLRAEFAPAWQPRGLRPETTIVPGHCAIVGSGLAGASVAASLARRGWRVTVLDEA
                     DAPAAGASSLPVGLLAPHFSVDDNLLSRLSRSGVRATLQQARMLLRQGQDWQASGVLE
                     HRFRDLPGVPRNAGAMAASAPQAAQDWSRGADAQQKAAAFLDPDAVAFWHQQAGWIKP
                     AALVRAWLAQPGIRWRGNVRVGRLLCADGGWGLFDARGGELERADLVVIAAGVATADL
                     LEGRLELQPVRGQVSWGIRDAQQRLPPFPVNGNGHFIPDVPLGDMAAWYCGASYGRGE
                     TDLAPRKADHQENLSRLGSLLPAVEQQLVGAFSTRAVKAWTGVRCASIDRRPLLGELA
                     PGLWVSTAMGSRGMTFAMLCAELLAARVHGEPLPLERRMAAALDALRHRH"
     gene            complement(2971..3738)
                     /locus_tag="VGA59_11860"
     CDS             complement(2971..3738)
                     /locus_tag="VGA59_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9720419.1"
                     /translation="MKSEKNVQRVFVKVVGFTDVERHALNTVFRLSEQRAIVYSLWMA
                     DAPAPPRLALIDGRSYEARLELAAVPDPQLNLIWVGAVAPANAQRTFVRPLSWPSVLN
                     AMDELFEQPDRIDFDLEFDLGADGTNPPDELRDTRKRALIASADRNERLYLRAKLALA
                     ELTQADEAETGAQALELARAHHYAVAVVDFALPDVNGWAFLKELMEARPAIPNVIMTK
                     AKASAGERLRAWIGGAKGCFDKPPHPGKLQDLLQKIC"
     gene            3919..4710
                     /locus_tag="VGA59_11865"
     CDS             3919..4710
                     /locus_tag="VGA59_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9720420.1"
                     /translation="MHDFAFVFAGFFVGLVVGLTGVGGGSLMTPLLIFGFGVKPYLAI
                     GTDLLFAATTKFGGSLGLARSRAIDWPVVALLSAGSIPAALVTIFFLHRIGPTSAAVQ
                     SVMTSTLGAALLLTAAATLYKAVKGKNTPRELDATQLIQATRPRHWSQPVLFGAAIGA
                     LVSLTSVGAGAIGVTVLMLLYPALPLPRIIAADIAYAVPLTLVAGLGHASIGSVDWTL
                     LGTLLAGSLPGIWLGSRLVRSAPDRLIRSLLSLLLAYAGARLISL"
     gene            4729..6579
                     /locus_tag="VGA59_11870"
     CDS             4729..6579
                     /locus_tag="VGA59_11870"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX9720421.1"
                     /translation="MYQYTDFDKQFVKTRAAQYRDQLERNLAGTLGDDEFRPLRLQNG
                     WYIQRYAPMLRIAVPYGEISSAQLRVLARIAREYDQPTAELFKDAQAKQDELGPVPLS
                     GGGSVRSHLKVGYGHFTTRQNVQFNWIPLVKSADVMDLLATVNMHGIQTSGNCIRNIT
                     TDERAGIAADEIADTRPFCELLRQWSTLHPEFAFLPRKFKIAFNGAREDRAAIGWYDV
                     GLQLLRSEGEAIHGAPAGELGFKVQVGGGMGRTPIIGTVVREFLPWNQLMNYLEAVIR
                     VYNRYGRRDNIWKARIKILVKAEGQRYIDQVEEEYRQIVEIDGATHTITQAELDRVAA
                     SFVPPALEHPRQRRGADANMHEILRAGALADPEYGRWLHRNVTGHKNPELRAVTLSFK
                     RLGHAPGDATGDQLDVAADLAERFSAGEARVTHEQNLLLPWVHEDDLPELWHAARMAG
                     LAKSNIGLLTDMISCPGGDFCSLANARSIPIAEAITQRYQDLDELHDIGEIDLHISGC
                     INSCGHHHSGHIGILGVDKDGKEWYQVTLGGADGSNLSGAATPGKVVGPSFSAAEVPE
                     VIEAVLDTYRQQRQSVGGKAETFIATLRRVGIEPFKAAANAARFTEKQVA"
     gene            6579..6974
                     /locus_tag="VGA59_11875"
     CDS             6579..6974
                     /locus_tag="VGA59_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEX9720422.1"
                     /translation="MKIMTQAITSGAEAPDELANVLSLANDADALSVSLEGVERIELH
                     FPKFTDGRAFSQAFLLRRRLGFRGDIRATGDVLIDQLLQMQRSGFSSAVLRADQDAAQ
                     AQGQLDRYGRFYQGDAVNLQPRFKEEAAA"
     gene            6971..7708
                     /locus_tag="VGA59_11880"
     CDS             6971..7708
                     /locus_tag="VGA59_11880"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX9720423.1"
                     /translation="MSAIDLHARASADFEAKLAETRSLLQRAAADFQPVTQASSLGAE
                     DVVITHLINSLALDIPVFVLDTGMLHEETLQLLERTKAGSQAPVTVYHPVNETVIHFV
                     GREGKDAMYKSVELRKACCHIRKVEPLERALAGKRAWITGLRREQSTNRAEVPLRDAG
                     DDATARIKFNPLANWTWGDVWHYIRLHQVDYNPLHDAFYPSIGCAPCTRAISLGEDFR
                     AGRWWWEDESAKECGLHVKQKKGEVPA"
     gene            7839..8771
                     /gene="cysD"
                     /locus_tag="VGA59_11885"
     CDS             7839..8771
                     /gene="cysD"
                     /locus_tag="VGA59_11885"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX9720424.1"
                     /translation="MNARVEPQLLGQLSNRHLDALEEEAIFILREVAAAFERPTLLFS
                     GGKDSLVLLKCAEKAFGSGRIPYPLLMIDTGHNFPEVTDFRDFRAAELGAELIVRHVE
                     GSMKRGTVRLAHPGESRNVHQSVTLLEAIEEFRFDALIGGARRDEEKARAKERIFSHR
                     DSFGQWQPKAQRPELWTLFNTRLHPGEHFRVFPISNWTELDVWQYIERENIALPSIYY
                     AHRRQVIERKGLLVPLTEMTPPKQGEDVVTRTVRFRTVGDITCTCPVESTAANASEVV
                     VETLTVEVSERGATRMDDKTSDASMEKRKKDGYF"
     gene            8771..10195
                     /locus_tag="VGA59_11890"
     CDS             8771..10195
                     /locus_tag="VGA59_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9720425.1"
                     /translation="MQDSVIAAKAAIHLPITKSLDPGLRRDDEAHHGDHVAALGTPRL
                     DPGLRRDNEARHGDHVAALRFITCGSVDDGKSTLIGRLLVDSKSVLQDQLAGVQRLGE
                     TDLALLTDGLSAEREQGITIDVAYRYFATPGRKFIIGDTPGHEQYTRNMVTAASSADA
                     AVVLVDATKLQWQDPKLELLSQTRRHSLLVNLLRVPSIVFAVNKLDAVEDPERAFGNI
                     SAALDAFTSAARIPVHAVVPISALKGHNVVAPQPGWCGYQGLSLLELLEQLDVAAAET
                     AEPFAFPVQWVEKFSSSSDISQGRRVFWGRVATGTVQPGQEVKVLPGGQTATVAQVLN
                     HAREPRNVPAGHGAGIVLDREVDVSRGDWLLSPGAAEPSRELSATIAWMDDEPLVAGR
                     VYWALHGHRWVKARVKRIVHKLDVNTLAEADADHLEPNSIGHVDLTLQEPLATLPYQR
                     SRILGSMVLVDTASHKTSGAVLVR"
     gene            10332..>10574
                     /gene="fdxA"
                     /locus_tag="VGA59_11895"
     CDS             10332..>10574
                     /gene="fdxA"
                     /locus_tag="VGA59_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX9720426.1"
                     /translation="MTHVVTESCIRCKYTDCVDVCPVDCFREGPNMLVIDPDECIDCA
                     VCIPECPVNAIFAEEDVPADQIAFVKINAELAQAPGW"
BASE COUNT         1802 a         3579 c         3433 g         1760 t
ORIGIN      
        1 atgagactgc ttcacatcga ttcgagcccc ctggccggca actcggtttc ccgccaactc
       61 accgagcgca tcgttgcgca atggcgcacc acccaccccg gcaccgtggt cgagtacctc
      121 gacctggcgg cggatgcgcc cagccacctg tccattgact cgttgggttt tcgcgccgga
      181 ccggacgcca aggatctgag cgaggtgcag cgccgcgaaa acgcgatctc cgagcgactg
      241 gtgagccagt ttctcgcggc tgacgtgatc gtggtgggtg cacccatgta caacttctcc
      301 attccgagcc agctcaaggc ctggatcgac cgcgtggcgc aagccgggcg caccttcacc
      361 tacaccgaga aaggccctaa gggcctggcc gccggcaaga ccgtgatcgt tgcctcgacc
      421 cgtggcggca tctactcctc gaacccggca atggctgggc tggaccacca ggagagctac
      481 ctgcagacgg tgttcggctt tttcggcatc gccgacgtgc agttcgtacg tgccgaggga
      541 gtcgcaatgg gcgatgcggc caaggcgcag gcactgagcg ccgcggacgt ggtcatcaag
      601 tcgctgtcgg ctgctccggc caacgagccc gaggcagcac tggccgcctg atcgctcgtc
      661 acctcgccat ggaacggcct gcgaaacgca ggccgttttc tttggtgacg ggcgttcggg
      721 gaaccgcgtc gggccgcaag aaggtcattg cgctggcggc ctgcgaatct tgataaagtg
      781 gcgaccctgt ccacccggaa atcatcaaga ggcgtctcat ggctcgtacc gttcaatgca
      841 tcaagctcgg caaggaagcc gaagggctgg atttcccgcc ttatcccggc gaactgggca
      901 agcgcctcta tgagaacgtg agcaagcagg cctgggccga ctggctcaag caccagacca
      961 tgctggtcaa cgagaaccgg ctgaaccttg cggaccagcg ggcgcgccag tacctggcgc
     1021 ggcagatgga gaaccatttc ttcggcggag gcgccgacgt ggcccagggt tacgtgccgc
     1081 ccagcgagta ggccccggcc cccaatcgtg aacgaaccgg ttgagtggct gcccgacggc
     1141 acgccgcgca gccctcgctt cgacgacatc tatcgctcca gcaccggcgg actcgaacag
     1201 gcccgccatg ttttcttgcg gggttgcggc ttgcccgatg cctgggcggg gcggccgcaa
     1261 tggcgcatcc tggaaacggg atttggcctg ggcctgaact tcctggcggc atggcgcgcg
     1321 tggaaagacg atccgcagcg gccgcggatg ctgcacttcg tgtcatcgga agcctggccg
     1381 gtttgcgccg ccgacctgct tcgcagcgcc gcgccccacc ctgaactggt tcccctggcc
     1441 acggcgctgg ccgagcagtg gttcggcctg gtggggggca tgcaccgcat ggccttcgag
     1501 gaaggactgg tcctgcttac gctgttcgtc ggcgatgtga aggctgccct gcgccagcag
     1561 cccttcaatg ccgactcgat cttcctcgat ggcttcgatc cgcagggcaa cgccgcgatg
     1621 tgggaacttc ctacgctcaa ggcggtggcg cggcactgcc ggcgcggcac gcggctggcc
     1681 acctggaccg tggcgggcga agtgcgccgc gggctgaccc agtgcggctt cgtcgtcgag
     1741 aaggcgcagg gcctggcgcc gaaacggcac tgcctgaggg ccgagttcgc accggcatgg
     1801 cagcccaggg gcctgaggcc ggaaaccacg atcgtgcccg ggcattgcgc catcgtgggc
     1861 tcgggccttg ccggcgcttc ggtcgcggcg agcctggccc ggcgcggctg gcgagtcacc
     1921 gtgctggacg aggccgatgc cccggccgcc ggggcctcaa gcctgccggt gggcctgctg
     1981 gccccgcatt tttcggtgga cgacaacctg ttgtcgcgcc tgagccgcag cggcgtgcgc
     2041 gccactttgc agcaggcgcg gatgctgctg cggcaaggac aggactggca agccagtggc
     2101 gtgctggagc accgcttccg cgatctgccc ggcgtgcctc gcaatgcggg ggccatggcc
     2161 gcaagcgcgc cgcaagcggc ccaagactgg agccgcggcg ccgacgcgca gcagaaagcg
     2221 gcggcttttc tcgatcctga tgcggtggcc ttctggcacc agcaggcggg ctggatcaag
     2281 ccggcggcgc tggttcgggc gtggctggcc caacccggca tccgttggcg cggcaacgtc
     2341 cgcgtgggac gcctcttgtg cgccgacggc ggctggggac ttttcgatgc gcgcggcggt
     2401 gaactggagc gtgccgacct ggtggtgatc gccgcggggg tcgcgactgc ggatctgctg
     2461 gaaggccggc tcgaattgca gccggtgcgc ggccaggttt cgtggggcat ccgggatgcg
     2521 cagcaaaggt tgccgccctt ccccgtgaac ggcaacggcc atttcatccc cgatgttccg
     2581 ctgggcgaca tggcggcctg gtattgcggc gccagctatg gccgcggcga aaccgatctg
     2641 gcgccacgca aggccgacca ccaggaaaac ctgagcaggc tgggctcgct gctgcccgcc
     2701 gtggagcagc aactcgtggg cgcgttttcc acccgtgccg tgaaggcatg gaccggcgta
     2761 cgctgtgcct ccatcgatcg ccggcccttg ctcggggaac tcgcgcccgg gttgtgggtg
     2821 agcacggcca tgggttcgcg cggaatgacc ttcgcgatgc tctgcgcgga gctgctggcg
     2881 gcgcgcgtgc atggagaacc gttgcccctg gagcggcgca tggccgccgc gctcgacgca
     2941 ttgcgacacc ggcactgaca cgtcgcctcg ctagcagatc ttctgcagca gatcctggag
     3001 cttgcccgga tggggcggtt tgtcgaagca gcccttcgcg ccgccgatcc aggcgcgcag
     3061 acgctcgccg gccgaggctt tcgccttggt catgatcacg tttggaatcg ccggccgcgc
     3121 ttccatcaat tccttgagaa aggcccagcc gttcacgtcg ggcagggcga aatccaccac
     3181 cgccaccgcg tagtgatggg ctcgtgccag ttccagcgcc tgggcgcccg tttccgcctc
     3241 atcggcctgg gtcagctcgg ccagggccag tttcgcgcgc aggtagaggc gctcgttccg
     3301 gtccgcgctg gcgatcaggg cgcgcttgcg cgtgtcccgc agctcatccg gagggttggt
     3361 gccgtcggcg ccaaggtcga attccaggtc gaaatcgatc ctgtcgggct gctcgaacag
     3421 ctcgtccatg gcgttgagga cactcggcca ggacagcggc cggacaaacg tccgctgggc
     3481 gttggccggg gcgaccgcgc ccacccagat gagattgagc tgggggtcag ggactgcggc
     3541 gagttccagc cgcgcttcgt agctccgtcc gtcgatcagc gccagcctgg gcggcgccgg
     3601 ggcgtccgcc atccaaagcg aataaacgat ggcacgttgc tccgacagcc gaaacacggt
     3661 gttgagcgca tgccgctcca catccgtgaa ccccacgacc ttgacaaaga cccgctggac
     3721 gtttttctcc gatttcatgt gttggctgtc gagctgtcgc cttatcttgc cagaagtagg
     3781 gggccttggc ggctttatcc acaactcgcg cgccgtcgcc tgccaggccg caccggtgct
     3841 ggcgcataag cttatccgta taaagccaga acgaaaaaat agtttgttcc cataaggcgt
     3901 ccccctaaag tcgcgctcat gcacgatttc gcgttcgtct ttgcgggatt ctttgtcggc
     3961 ctggtggtcg ggctgaccgg cgtcggcggc ggctcgctga tgacgccgct gctcatcttc
     4021 ggcttcggcg tcaagcccta cctggccatc ggcaccgatc ttctgtttgc cgccacgacc
     4081 aagttcggcg gctcgctcgg gctcgcgcgc tcgcgggcca tcgactggcc ggtggtcgcc
     4141 ctgctgtccg ccggcagcat cccggccgcg ctggtgacga tcttctttct ccatcgaatc
     4201 ggccccacca gtgccgctgt ccagtccgtg atgacctcga cgctcggcgc cgcgctgttg
     4261 ttgaccgccg ccgcgacact gtacaaggca gtgaagggca agaacacacc gcgcgaactc
     4321 gatgcaacgc agttgatcca ggcgacccgg ccgcgccact ggagccagcc ggtgctgttc
     4381 ggtgccgcca tcggcgccct ggtttcgctc acatccgtcg gcgccggggc gatcggtgtc
     4441 acggtgctga tgctgctcta tcccgcgctg ccgctgcccc ggatcatcgc cgccgacatt
     4501 gcctatgccg tgccgctgac cctggtggcg ggcctgggcc acgcctcgat cgggtcggtc
     4561 gactggaccc tgctgggcac cttgctcgca ggctcgctgc ccggcatctg gctcggttcg
     4621 cgcctggtgc gcagtgcgcc tgaccggctg atccgctcgc tgctttccct cctgctggct
     4681 tacgccggtg cccggctgat ctccttgtag cgcccgagag ccaagaacat gtaccaatac
     4741 acagacttcg acaagcagtt cgtcaagacg cgggccgccc aataccgcga ccagttggag
     4801 cgcaacctgg ccggcaccct gggagacgac gagtttcggc cgctgcggct gcagaacggc
     4861 tggtacatcc agcgctatgc cccgatgctg cgcatcgccg tgccctacgg cgagatttcc
     4921 agcgcgcagt tgcgcgtgct ggcgcggatc gcccgcgaat acgaccagcc gacggccgaa
     4981 cttttcaagg atgcccaggc caaacaggat gagctgggac ccgtgccgct atccggcggc
     5041 ggctccgttc gctcgcacct gaaagtggga tacggccatt tcaccacccg ccagaacgtg
     5101 cagttcaact ggattcccct ggtgaagagc gcggacgtga tggacctcct ggccaccgtc
     5161 aacatgcacg gcatccagac cagcggcaat tgcatccgca acatcaccac cgacgagcgc
     5221 gccggcatcg cggcggacga gatcgccgat acccgcccgt tctgcgagct cctgcgccag
     5281 tggagcacgc tgcatcccga gttcgctttc ctgccgcgca agttcaagat cgctttcaac
     5341 ggcgcgcggg aagaccgtgc cgccatcggc tggtacgacg tcggcctgca gctcctgcgc
     5401 agcgaaggcg aagcgattca tggcgcaccg gccggcgaac tgggcttcaa ggtgcaggtg
     5461 ggcggcggca tgggtcgcac gccgatcatc ggcacggtgg ttcgcgagtt cctgccctgg
     5521 aaccagctca tgaactacct cgaggccgtg atccgcgtct acaaccgcta cggccggcgc
     5581 gacaacatct ggaaagcgcg catcaagatc ctggtgaagg cggagggcca gcgctatatc
     5641 gaccaggtgg aagaagagta ccggcagatc gtcgagatcg acggcgccac gcacaccatc
     5701 acgcaagccg agctggaccg cgtcgcggcc tcgttcgtgc cgcccgcgct cgagcacccg
     5761 cggcagcgca gaggcgccga tgcgaacatg cacgagatcc tgcgcgccgg cgcgctggcc
     5821 gacccggagt acggccgctg gctgcaccgc aacgtcaccg gccacaagaa ccccgagctg
     5881 cgcgccgtca ccttgtcgtt caagcgcctc gggcatgccc ccggtgatgc cacgggtgac
     5941 cagctggacg tcgcggccga cctggccgag cgcttctcgg ccggtgaagc ccgggtcacg
     6001 cacgagcaga acctgctgct gccctgggtg cacgaggacg acctgcccga actctggcac
     6061 gccgcccgca tggccggcct cgccaaatcc aacatcggcc tgctgaccga catgatctcc
     6121 tgccccggcg gcgacttctg ctcgctcgcc aatgcgcgtt cgatccccat cgccgaagcc
     6181 atcacccagc gctaccagga cctggacgag ctgcacgaca tcggcgagat cgacctgcat
     6241 atctccggct gtatcaactc ctgcggccac caccacagcg gccacatcgg catcctgggc
     6301 gtcgacaagg acggcaagga gtggtaccag gtgacgctgg gaggcgccga cggctccaac
     6361 ctgtcgggcg cggccacgcc gggcaaggtt gtcggcccct cgttctccgc cgccgaagtg
     6421 cccgaagtga tcgaagccgt gctggacacc tatcggcaac aacgccagag cgtgggcggc
     6481 aaggccgaaa cctttatcgc cacgttgcgg cgcgttggca tcgagccctt caaggcagct
     6541 gccaacgccg cccgtttcac tgaaaagcag gtggcgtaat gaaaataatg acacaggcga
     6601 taacgtccgg cgccgaggcg ccggacgaac ttgcgaatgt cttgtctctg gcgaacgatg
     6661 cggatgcgct gtcggtatcg ctggagggcg tggagcgcat cgagctgcac tttcccaagt
     6721 tcaccgacgg ccgcgcgttc agccaggctt tcctgctgcg ccgcaggctc ggcttccggg
     6781 gcgacatccg cgccaccggc gacgtgctga tcgaccagct gctgcagatg cagcgcagcg
     6841 gtttttccag cgccgtgctg cgcgccgacc aggacgccgc ccaagcgcag ggccagctgg
     6901 accgctacgg ccgcttctac cagggtgatg ccgtcaacct gcaacctcgc ttcaaggagg
     6961 aagcagcggc atgagcgcga tcgacttgca tgcgcgcgct tccgccgatt tcgaagcgaa
     7021 gctggccgag acccgcagcc tgctgcaacg cgcggcggcc gatttccagc ctgtcaccca
     7081 ggcgtccagc ctgggcgcgg aagacgtggt catcacacac ctgatcaaca gcctggcact
     7141 ggatattccg gtcttcgtgc tggacacggg gatgctgcat gaagagacgc tgcagctgct
     7201 ggagcgcacc aaggccggct cgcaggcccc ggtgacggtg taccacccgg tcaacgaaac
     7261 ggtgattcat ttcgtgggcc gcgaaggcaa ggacgcgatg tacaagagcg tcgagctgcg
     7321 caaggcctgc tgccacatcc gaaaggtcga accgctcgag cgggcgctgg ccggcaagcg
     7381 cgcctggatc acaggcctgc gccgcgagca gtccacgaac cgcgccgagg taccgctgcg
     7441 cgacgccggt gacgatgcca cggcccgcat caagttcaac cccctggcca actggacctg
     7501 gggcgacgtc tggcactaca tcaggctgca ccaggtcgac tacaacccgc tgcacgacgc
     7561 tttctatccc agcatcggct gcgcgccctg cacccgcgcc atcagcctgg gcgaggattt
     7621 ccgcgccggc cgctggtggt gggaggatga atccgccaag gagtgcggac tgcacgtgaa
     7681 gcagaaaaaa ggcgaggtcc cagcatgacc ccctgccttg tcatcccggc cccggatcag
     7741 gtccggggtt ccagcacccg ggatccatgc ctcgcatcgc aatcattttt tcaactccaa
     7801 catggattgc ggatcaagcc cgcaatgaca caccgcctat gaacgcgcgc gtcgaacccc
     7861 aactgctggg ccagctgagc aaccggcacc tggatgcact ggaagaagaa gccatcttca
     7921 tcctgcggga agtggcggcc gccttcgagc gcccgaccct gctgttctcg ggaggcaagg
     7981 attcgctggt gctgctcaag tgcgctgaaa aggccttcgg gagcggccgc attccctatc
     8041 cactcctgat gatcgacacc ggccacaatt tcccggaagt cacggatttc cgcgacttcc
     8101 gcgccgccga gctcggcgct gaattgatcg tgcgccatgt cgagggctcg atgaagcgcg
     8161 gcacggtgcg gctggcccac ccgggcgaat cgcgtaacgt ccaccagtcc gtgacgctgc
     8221 tggaagcgat cgaggaattc cggttcgacg ccctgatcgg cggtgcgcgt cgcgacgagg
     8281 aaaaagcccg cgccaaggaa cgcatcttct cgcaccgcga cagcttcggc caatggcagc
     8341 ccaaggccca gaggcccgaa ctgtggacgc tgttcaacac ccgcctgcat ccaggcgagc
     8401 acttccgcgt attccccatt tccaactgga cggagctgga tgtctggcag tacatcgaac
     8461 gcgagaacat cgcgctgccc tcgatctatt acgcccaccg tcgccaggtg atcgagcgca
     8521 agggcctgct ggtgccgctt acggaaatga cgcccccaaa acaaggcgag gacgtcgtaa
     8581 cgcgcaccgt gcgtttccgc accgtgggcg acatcacttg cacctgcccg gtggaaagca
     8641 cagcggccaa cgcctccgaa gtggtggtgg agacgctgac cgtcgaagtc agcgagcgcg
     8701 gtgccacccg catggacgac aagacatcgg acgcctccat ggaaaagcgc aagaaggacg
     8761 ggtatttctg atgcaagact ctgtcattgc ggcgaaggcc gcaatccacc tcccgatcac
     8821 caagagcctg gatcccggcc ttcgccggga tgatgaagct caccacggcg accatgtcgc
     8881 cgcgctgggc acaccgcgac tggatcccgg ccttcgccgg gataatgaag ctcgccacgg
     8941 cgatcatgtc gccgcgctgc gcttcatcac ctgcggctcg gtggacgacg gcaagagcac
     9001 gctgatcggc cggctgctgg tggacagcaa gtcggtgctt caggaccagc tggccggggt
     9061 gcagcgactc ggtgagaccg atctggcgct gctgaccgac ggcttgtcgg ccgagcgcga
     9121 acagggcatc accatcgacg tggcctaccg ctacttcgcc acgcccggac gcaaattcat
     9181 catcggcgac acaccgggcc atgaacagta cacgcgcaac atggtcacgg ccgcctccag
     9241 tgccgacgcc gccgtggtgc tggtggacgc caccaagctg cagtggcagg acccgaagct
     9301 ggaactgctg tcgcagaccc gccgccattc gctgctggta aacctgctgc gcgtgccttc
     9361 gatcgtcttc gccgtgaaca agctcgatgc ggtcgaggat ccggagcgcg ccttcggcaa
     9421 catcagcgcc gccctggacg cctttacctc ggccgcccgc attccggttc atgccgtcgt
     9481 gccgatctcc gcactcaagg gtcacaacgt cgtcgccccg cagcccggct ggtgcggcta
     9541 ccagggcctc tcgctgctgg aactgctgga acagctggat gtcgcggctg ccgagacggc
     9601 tgaaccgttc gcattcccgg tgcagtgggt cgagaagttc tcttcgtctt ccgacatcag
     9661 ccagggccgc cgcgtgttct ggggccgcgt ggccacgggg accgtgcagc ccggccagga
     9721 agtcaaggtg ctgcccggcg gccagaccgc gacagtggct caggtgctca accatgcgcg
     9781 cgagcctcgg aacgtgccgg ccggccacgg ggccggcatc gtgctggacc gcgaggtgga
     9841 cgtgtcgcgg ggcgactggc tgctgtcgcc gggggcggcg gagccttcgc gcgagctatc
     9901 ggccacgatc gcctggatgg acgacgagcc gctggtcgcc ggccgcgttt actgggcgct
     9961 gcacggccac cgttgggtca aggcgcgcgt gaagcgcatc gtgcacaagc tggacgtcaa
    10021 cacgctggcc gaagccgacg cggaccacct cgagcccaac tccatcggac acgtggacct
    10081 gacgctgcaa gagccgctgg ccaccctgcc ctaccagcgc tcgcgcattc tcgggtcaat
    10141 ggtgctggtg gacacggcca gccacaagac ctcgggggct gtgctggtac gttaggcgcg
    10201 cggccttttt ctgatgaaat gcaatcaagg gccctgccgt gggcgcaggg tcgggaccgt
    10261 cctcgggacg gccttgccgg tcgttggcta aaattgaacg tttcccccag cactaagcat
    10321 caagcataaa catgactcac gtcgtcaccg aatcctgcat ccgctgcaag tacaccgact
    10381 gtgtggacgt ctgccccgtg gattgtttcc gcgaagggcc caacatgctg gtgatcgatc
    10441 ccgacgaatg catcgactgc gcggtgtgca ttccggagtg cccggtcaac gcgatctttg
    10501 ccgaggaaga cgtgcccgcc gaccagatcg ccttcgtcaa gatcaacgcc gaactggcgc
    10561 aggcgcccgg ttgg
//
LOCUS       DASWIY010000334        17447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1323577, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000334 DASWIY010000000
VERSION     DASWIY010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 17447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17447
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323577"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VGA59_11900"
     CDS             <1..691
                     /locus_tag="VGA59_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720427.1"
                     /translation="IVRAKALIARAGQLDNRDAKWNVIDVLLNAAAKVLSDYKQSGTK
                     DPNWHVQMLALAKLRDTPSGKVIELVKQAIESDPQAFATYKAASLALAFDKGWAKPDL
                     VEWMARLAVEKTKDSEAQSRYALIYGDAAVWFMELRIRPFGPGRAEWSTLNQAFKDVE
                     KYHPKLYKLNLHAAFACLAGDKNSTSYLIKRVGTDVRREDWFLGRRDDWATGREYDRC
                     KRWSMMGDKTA"
     gene            complement(748..1863)
                     /locus_tag="VGA59_11905"
     CDS             complement(748..1863)
                     /locus_tag="VGA59_11905"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX9720428.1"
                     /translation="MEAFQHSAALSLGVELELQLVNTHDYDLAPYSDEMLRLMSRYSL
                     PGAVVPEMTSSMIEISTGVCQTSSQVLAELSEIRDALVKCADKLNIAVVGGGTHPFQQ
                     WHERRIYDKPRFRELSELYGYLSKQFTIFGQHVHVGCPGADSALLMLHRMSRYIPHFI
                     ALSASSPFVQGQDTQFDSARLNSVFAFPMSGRAPFTLSWDEFAKFFAKTTRTGVVKSM
                     KDFYWDIRPKPEYGTIEIRVFDTPLTVDRAAALAGFVQSLASWFLHEQPFMPQEDDYM
                     VYTYNRFQACRFGLDAVYVDPPTGQHMPLRDHIIQTLRQIGDHARQVGASHGVQLLGE
                     DAELGTNDARWQRENQARERLLAEVVRQGALRFRGQA"
     gene            complement(1907..3166)
                     /locus_tag="VGA59_11910"
     CDS             complement(1907..3166)
                     /locus_tag="VGA59_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9720429.1"
                     /translation="MNEIMAIWAEWLKPSAGLPTVQWSLLLAAAAAAGHLLQRHTGLP
                     KVVGYTVTGALAGLAGFSGAAWPLQGIALFLLELAVSVVLFEAGGRLALRWFRHNPMV
                     LLQSLLESTLTALFVYYSLRWMGVRPAVAEALGLIAMVASPAVLSRVMMDTQASGPVT
                     ERAMVLSTLSSLYALTLVSARAGVMHRPTTSLSETLFPVAVVLGMSLVVAGLLALALR
                     TALRVMSPTSENTSMLLIALIAAATALAAHFGGFAPLAALLGGILLKQLNPRPWAWPR
                     QLGNAASLLTMLMFVLVSVVAAKADWNPAVAGLVAALVLARGAAKVIGVGAANWGTGT
                     SWRQAFWTGCAMSPMSSVALLLVQQFMTSSLTLGPRIASIALPAILLMEVLGAIIVTL
                     AIYRVGESSRSLPPEPIQVTQPGDLRG"
     gene            complement(3246..3626)
                     /gene="apaG"
                     /locus_tag="VGA59_11915"
     CDS             complement(3246..3626)
                     /gene="apaG"
                     /locus_tag="VGA59_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX9720430.1"
                     /translation="MAKYRFRVEVEPHYLPEQSAPEQNLYSFAYTITITNTGEVPAQL
                     VSRHWIIADALGHIEEVKGLGVIGQQPLLKPGEAFQYTSGCRLRTASGTMQGSYFCVA
                     EDGARFETEIPAFVLDDGASRVLH"
     gene            3668..4351
                     /gene="rpe"
                     /locus_tag="VGA59_11920"
     CDS             3668..4351
                     /gene="rpe"
                     /locus_tag="VGA59_11920"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX9720431.1"
                     /translation="MSTIHRIAPSILSADFARLGDEVAKVIAAGADWIHFDVMDNHYV
                     PNLTFGPMICSALKPHAVTAAGHAVPIDVHLMVEPVDALAQAFAQAGADYISFHPDAS
                     AHVHRSVQAIKATGCKAGLVFNPASSMDVLDWMIEEIDLILIMSVNPGFGGQGFIESA
                     LRKIELARQRITDCGKDIRLEVDGGIKTDNIARVAAAGADTFVAGSAIFGKPDYKGVI
                     DAMRAALKG"
     gene            complement(4341..4646)
                     /locus_tag="VGA59_11925"
     CDS             complement(4341..4646)
                     /locus_tag="VGA59_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720432.1"
                     /translation="MHNRIPSRRRQGPSKGKGPARQGNTMPAQGDTRNRVPRMPHERD
                     ESADSQGASEPSGPRMAKLAQEDIERGLVDTDKGPVMDETYEKLREESSDPVKKFSP"
     gene            4925..5047
                     /locus_tag="VGA59_11930"
     CDS             4925..5047
                     /locus_tag="VGA59_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720433.1"
                     /translation="MELIAGMPEWLVWCAAGGAGVVALASLANILDAAFELDAS"
     gene            5124..7283
                     /locus_tag="VGA59_11935"
     CDS             5124..7283
                     /locus_tag="VGA59_11935"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX9720434.1"
                     /translation="MPQSKQDAQRKSKAANGAAAKQKQLESFSQGPENLLTTNQGVVI
                     PDNHNSLKAGVRGPSLLEDFILREKIAHFDHERIPERVVYAQGAAAHGYFELTRSLVE
                     YTRADFLQEAGARTPVFVRFSAASGSRGSADSLRDSRGFAVKFYTREGNYDLVGGSMP
                     VFFIQDAIKYPDLIHAMKPEPHHGMPQAATAHDSFWDFASLTPETTHMLMWLMSDRAI
                     PRSYRMMEGFGVHTFRLINAKGVAHFVKFHWKPRLGKHALVWDEAQKIAGKDPDFHRR
                     DLWEAIVNGNFPEWELGLQLIEESKADSLGFDLLDPTKLIPEEIVPVLAVGKLVLNRN
                     PDNFFAETEQVAFHPGHVVPGIDFSNDPLLQGRLFSYTDAQLSRLGGPNFHELPINRG
                     LCPFHNFQRDGMHRMLVNKGQVAYEPNSLASGAEFRVDGGRQGFQSYPEALESPKTRR
                     RSPSFDDHFSQATLFWNSQGPAEKEHIIAAFQFELSKVELVAVRQRVVDNLAHVDAKL
                     ARKVAEPLGIALPDPKAAAGRAGFRDMRTELPLETSAALSMEGGAAGIATRRVAVLVA
                     GGVEVGALRVIQQALQDAGAASRIVAAHLGFVATSSGQQLAVDHTFANMPSVMFDAVL
                     VPGGAASAEALARDGDAVHYALEAYKHCKTLCVIGESVQLLRTLGIGAGEGGPAVAGV
                     LIGKNDPTGRAQLAQDFIAAIARHRHWMRPNLEAIPA"
     gene            7433..7849
                     /locus_tag="VGA59_11940"
     CDS             7433..7849
                     /locus_tag="VGA59_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEX9720435.1"
                     /translation="MAASNQGNQGKGQQGGSSNRGFASMDPERQREIASEGGRAAHQK
                     GTAHEFSSEEARQAGSKGGLAAHEKGTAHEFDSEEAREAGRKGGQASHEKGTAHEFDS
                     EEAREAGRKGGQASRGGGSQVGANQGGDQGGSSGKR"
     gene            7978..8664
                     /gene="gph"
                     /locus_tag="VGA59_11945"
     CDS             7978..8664
                     /gene="gph"
                     /locus_tag="VGA59_11945"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655723.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEX9720436.1"
                     /translation="MTPIAAQAAIVDLDGTMVDTLGDFAVALNRVLDELSLPGIERSE
                     IERMVGKGSPHLIRSMLAHVRAAPSLYEQAWQSYRKHYLAINGQYASVYPGVTEGLRT
                     LKAAGLKLACLTNKPIAFAGPLLEAKGLREYFQLVFGGEAFERQKPDPLPLLKTCEAL
                     GSAPARTLVIGDSSNDARAARAAGCPVLLVTYGYNHGEPVRAVDADGFLDSLAEVPGW
                     LRRPYPTALP"
     gene            complement(8633..9217)
                     /locus_tag="VGA59_11950"
     CDS             complement(8633..9217)
                     /locus_tag="VGA59_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEX9720437.1"
                     /translation="MSLLKRWLFATATFLLASAAFAQVTVSGVKFDQSADVRGTKLQL
                     NGAGTRYKAIFKVYAAGLYLPTKGGTPEQVLAMPGAKRLNITMLREIDSSEFGKMFSR
                     GMEDNMEKAAFSKLIPGVLRMSQVFSDHKKLGPGDTLSLEWVPGTGTLLTVKGVPQGE
                     PFKEPEFFNALLRIWLGPNPADWKLKEALLGKAA"
     gene            9559..11058
                     /gene="trpE"
                     /locus_tag="VGA59_11955"
     CDS             9559..11058
                     /gene="trpE"
                     /locus_tag="VGA59_11955"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX9720438.1"
                     /translation="MITELEFKSLGLQGYNRIPLIAEAFADLETPLSLYLKLAHAKGG
                     GKHSFLLESVVGGERFGRYSFIGLPARTLLRCSGFGADAVTQVVEDGQVVETARGNPL
                     DFIAEYQKRFKVALRPGLPRFCGGLAGYFGYDTVRYIEKKLENSCPSDTLGCPDILLL
                     QCEELAVIDNLSGKLYLIVYADPGQPEAYANGKKRLRELREQLKYSVSAPVIRQTQTF
                     AAEREFAKADFLKAVERAKELIAAGDFMQVQVGQRIKKRYTESPLSLYRALRSLNPSP
                     YMYYYHFGDFHVVGASPEILVRQEQTPEGQKVTIRPLAGTRPRATTPEGDKAIEQELI
                     NDPKERAEHVMLIDLARNDIGRIAKTGTVKVTEAFAVERYSHVMHIVSNVEGILNDGM
                     TSIDVLKATFPAGTLTGAPKVHAMELIDQLEPTKRGLYGGACGYLSYAGDMDVAIAIR
                     TGIVKDQTLYVQAAAGIVADSVPELEWKETEAKARALLRASELVEEGLE"
     gene            11141..11713
                     /locus_tag="VGA59_11960"
     CDS             11141..11713
                     /locus_tag="VGA59_11960"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX9720439.1"
                     /translation="MRLLMIDNYDSFTYNLVQYFGELGAQVEVFRNDEITVGGIAQRK
                     PDRLVVSPGPCTPAEAGISVEAIRHFAGKLPILGVCLGHQAVGAAFGGNIVRARQLMH
                     GKTSVITTTGEGVFAGLPEKFTVNRYHSLAIEKSSCPGELATTAWTDDGEIMGVRHKQ
                     LAIEGVQFHPESILTEHGHAMLRNFLEQSK"
     gene            11787..12836
                     /gene="ltaE"
                     /locus_tag="VGA59_11965"
     CDS             11787..12836
                     /gene="ltaE"
                     /locus_tag="VGA59_11965"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEX9720440.1"
                     /translation="MMATIVDLRSDTVTRPTPGMRAAMDSAPLGDDVFGDDPSVNALQ
                     DKIAGMLGFEAALFVPTGTQSNLCAILSHCQRGDEYIVGQMQHCYRWEAGGGAVFGSV
                     QPQPLEHRADGSLALADIAAAIKPDDPHFARTRMLALENTLGGKLLPFAFIEQATELA
                     QRSGLARHLDGARLFNAAVAQAAQTGGDARAEARRIAQRFDSVSVCFSKGLGAPVGSA
                     LCGSRELIARARRVRKMAGGAMRQAGILAAAASYALDHHVDRLAQDHALALRLAQGLG
                     DIENLRVEAPQTNMVFMDLVGPAREESKALLKHLASHGILATGLYRLRFATHLDLDAN
                     GVDRAVAAIRQYFGK"
     gene            12897..13928
                     /gene="trpD"
                     /locus_tag="VGA59_11970"
     CDS             12897..13928
                     /gene="trpD"
                     /locus_tag="VGA59_11970"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX9720441.1"
                     /translation="MPITPQEALQRTIEHREIFHDEMLKVVRMIMSGELSPVMMAALI
                     TGLRVKKETIGEITAAAQVMREFSTKVNVADKTNLVDIVGTGGDGSHTFNISTCSMFV
                     AAAAGAKVSKHGGRSVSSKSGSADVLESLGMNINLPPDAIARCIAEVGIGFMFAPNHH
                     PAMKNVAPVRKELGVRTIFNILGPLTNPAGAPNILMGVFHPDLVGIQVRALQRLGTDH
                     ALVVYGRDGMDEVSLGAATMVGELKNGEISEYEIHPEDFGLPMASNRTLKVETPEQSR
                     QMLTGVLDNEAGAARDIVILNAGAALYAANVADSIQAGIGKAREALASGAAKAKLEQL
                     VKLSQTLAA"
     gene            13932..14723
                     /gene="trpC"
                     /locus_tag="VGA59_11975"
     CDS             13932..14723
                     /gene="trpC"
                     /locus_tag="VGA59_11975"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX9720442.1"
                     /translation="MPDILDRIVAVKREEIAAAQEKVPFAAMRMDAESRVLTRDFEGA
                     LRRKIAAGKSAVIAEIKKASPSKGVLRAEFIPADIAQSYAESGAACLSVLTDRQFFQG
                     QVDDLKQARASCDLPVLRKDFMVDAYQIYQSRAIGADCILLIAACLEDARMAELEAVA
                     RSLDMAVLVEVHDRSELERALKLKTPLVGINNRNLHTFEVTLDATLDLVKEVPADRLL
                     VTESGIVTKADVNKMRAAGVHAFLVGEAFMRADDPGEALAALFGD"
     gene            14795..15514
                     /locus_tag="VGA59_11980"
     CDS             14795..15514
                     /locus_tag="VGA59_11980"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9720443.1"
                     /translation="MREPDRLLRWEPEPWQLAEGWRDIVEAFLSSTAGLRLASYIRER
                     LAAGATIYPPRPFRALELTPLDSVRAVILGQDPYHGPGQAEGLAFSVRAGVKLPPSLR
                     NIFKELHGADDESQPPTGSLVDWARRGVLLLNASLTVEDGQPGSHARKGWEVLTDALL
                     AEVAARASPCVYMLWGAHAQAKAALIENTAARHGCEALVLQANHPSPLSAGRPPVPFL
                     GCGHFALARDWLAARGYPGVF"
     gene            complement(15529..16671)
                     /locus_tag="VGA59_11985"
     CDS             complement(15529..16671)
                     /locus_tag="VGA59_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9720444.1"
                     /translation="MRTLNLALQGGGSHGAFTWGALDALLADDRIAIEGLSGASAGAV
                     NAVALASGYAKGLAAGHDPRQSARESLGRVWHEISDWGTLGAMQQRMVGMLWGGLPPE
                     LAPANIMAKAWRSLLSPYQSNPLDINPLRSLLEREIDFDAIAKSRQLKVFVSATHVNT
                     GRAVLFTGQRLTVQAVMASACLPMLFRAVPIDGENYWDGGYSVNPPLAPLIEACESRD
                     LLLVQINPLKRERVPQSPTEILDRINELTFNASLLTQMRSIDFINRLIEEGALRGGRC
                     KHVLLHRIDGGADMAHYPASSRTSTDAVMIGKLFELGQASATQWLARHFAALGRRSTI
                     DIRRDYLDDTREETLRPQGPGRPQRGFRPWLARLFRGSWRVGQVRN"
     gene            16831..16916
                     /locus_tag="VGA59_11990"
     tRNA            16831..16916
                     /locus_tag="VGA59_11990"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16865..16867,aa:Tyr,seq:gta)
     gene            17051..17124
                     /locus_tag="VGA59_11995"
     tRNA            17051..17124
                     /locus_tag="VGA59_11995"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17083..17085,aa:Gly,seq:tcc)
     gene            17158..17232
                     /locus_tag="VGA59_12000"
     tRNA            17158..17232
                     /locus_tag="VGA59_12000"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17190..17192,aa:Thr,seq:ggt)
     gene            17312..>17447
                     /locus_tag="VGA59_12005"
     CDS             17312..>17447
                     /locus_tag="VGA59_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9720445.1"
                     /translation="MAKAKFERTKPHVNVGTIGHVDHGKTTLTAAITSVLAAKFGGEV
                     K"
BASE COUNT         3140 a         5673 c         5781 g         2853 t
ORIGIN      
        1 aattgtgcgc gccaaggcgc tgattgcgcg cgcaggccaa ctcgacaaca gagatgcgaa
       61 atggaatgtc atcgatgtgc ttctgaatgc cgcggctaag gtgctgagtg actacaagca
      121 gtctggaacc aaggatccca attggcacgt gcaaatgctt gccctcgcga agctgcggga
      181 tacgccctcg ggaaaggtga tcgaacttgt gaagcaggcg atcgaaagcg atccacaggc
      241 ctttgccacc tataaagccg ccagtcttgc cttggcgttc gacaaagggt gggcaaagcc
      301 cgacttggtg gagtggatgg cccgcctggc agtcgaaaag acgaaggatt cggaagcgca
      361 aagccggtat gcgctgatct acggggatgc ggcggtttgg ttcatggaac tgcgcatcag
      421 acccttcggg ccgggtcggg ccgaatggtc cacgctgaac caggcgttca aagacgtgga
      481 gaagtaccac cccaaactct acaagttgaa tctgcacgcc gcctttgcct gtttggccgg
      541 agacaagaat tccacgtcgt atctgatcaa gcgcgtcggc actgatgtcc gtcgagagga
      601 ttggttcttg gggcggaggg acgactgggc tactggaagg gagtacgacc gctgcaagcg
      661 ctggtcgatg atgggtgata aaacggcttg agccgagcag gcgtcgcgaa ccggcccagg
      721 cgagaccatc agcccggatg atgtcgtcta cgcctgtccc cgaaaccgca acgccccctg
      781 cctcaccacc tccgccagca gcctttcccg cgcctggttc tcgcgctgcc agcgcgcatc
      841 gttggtgccc agctccgcgt cttcgccgag cagctgcacg ccatggctgg cgccgacctg
      901 gcgagcatgg tcgccgatct ggcgcaaggt ctggatgatg tgatcgcgca agggcatgtg
      961 ctggccggtg ggcggatcca cgtagaccgc gtccagcccg aaccggcagg cctggaagcg
     1021 gttgtaggta tagaccatgt aatcgtcttc ctgcggcatg aagggctgct cgtgcaggaa
     1081 ccacgaggcc agcgattgca cgaaccccgc cagcgcggcg gcgcgatcca ccgtcagcgg
     1141 cgtgtcgaac acccggatct cgatcgtgcc gtactcgggc ttgggccgga tgtcccagta
     1201 gaagtccttc atgctcttca caacccccgt tcgcgtggtc ttggcgaaga acttggcgaa
     1261 ctcgtcccag ctcagggtga atggcgcgcg gcccgacatc gggaaggcga acaccgaatt
     1321 cagccgcgcc gagtcgaact gcgtgtcctg tccctgcacg aacggcgagg atgccgacag
     1381 cgcgatgaag tgcgggatgt agcgcgacat gcggtgcagc atcagcagcg cgctgtccgc
     1441 gcccggacag ccgacgtgca cgtgctgccc gaagatggtg aactgcttgg acaggtagcc
     1501 gtacagctcc gagagctcgc gaaaacgcgg cttgtcgtaa atgcgccgct cgtgccattg
     1561 ctggaacggg tgcgtgccgc cgccgacgac ggcgatgttg agcttgtcgg cgcacttgac
     1621 cagtgcatcg cgtatttccg acaactcggc cagcacctgc gacgacgtct ggcagactcc
     1681 ggtggagatc tcgatcatgc tcgaggtcat ttccggcacc accgccccgg gcaacgaata
     1741 gcgcgacatc agccgcagca tttcgtcgga gtagggcgcc aggtcgtagt cgtgcgtgtt
     1801 gaccagctgc agctccagct cgacccccag cgacagcgcc gccgagtgct ggaacgcctc
     1861 caggcagggt ggctccccgg tcttcgccgc cggaggtcgc accggcttat ccacgcaagt
     1921 ctccgggctg ggtgacttgg atgggctcgg gcggcagcga cctggaactc tcgcccacgc
     1981 ggtagatggc caaggtgacg atgatcgcgc cgagcacctc catcagcagg atcgcgggca
     2041 gcgcgatgct cgcaatgcgc ggccccagcg tgagcgagga agtcatgaac tgctgcacca
     2101 gcagcaaggc caccgacgac atcggcgaca tggcgcagcc ggtccagaat gcctggcgcc
     2161 agctggtgcc ggtgccccag ttggcagcgc ccacgccgat cactttggcg gcgccgcgcg
     2221 ccagcaccag cgccgcgacc agcccggcca cggccgggtt ccagtcggcc ttggcggcga
     2281 ccaccgatac cagcacgaac atcagcatcg tgagtagcga cgccgcgttg cccagctgcc
     2341 gcggccaggc ccaggggcgc gggttgagct gtttgagcag gatgccgccg agcagggccg
     2401 ccaatggcgc gaagccaccg aaatgcgccg ccagggccgt cgcggcggcg atcagggcaa
     2461 tcagcagcat cgaggtgttc tcgctggtcg ggctcatcac gcgcagcgcc gtgcgcagcg
     2521 ccagcgcaag caatcccgcg accacgagcg acatccccag gaccaccgcc accggaaaca
     2581 gcgtctcgct caagctggtg gtgggcctgt gcatcacgcc ggcgcgggcg ctgaccagcg
     2641 tcagcgcgta cagcgagctc agtgtggaca gcaccatggc gcgctcggtc accggccccg
     2701 aagcctgggt gtccatcatc acgcgggaga gcaccgcagg ggacgccacc atggcgatca
     2761 ggcccagggc ttcggccacc gcgggccgca ctcccatcca gcgcaagctg taatagacga
     2821 acaaggcggt gagggtggat tccagcaggc tctgcaacag caccatgggg ttgtgccgga
     2881 accagcgcag ggcgagccgc ccgccggctt cgaacagcac gacggagacg gccagttcca
     2941 gcagaaacag cgcaatcccc tgcagcggcc aggccgcccc ggaaaatccg gccagtcccg
     3001 ccagcgcgcc ggtcacggta tagccgacca ccttgggcag gccggtatgc cgttgcagca
     3061 ggtggccggc cgcggccgcg gccgccagca gcagcgacca ttgcacggtg ggcaggccgg
     3121 cggagggttt gagccattcg gcccagatgg ccatgatttc gttcatgcac gtctcccccc
     3181 ggcgcggact gtaccgcggc ctgagccgcg accgctgtag gactgggcgc cgaaggcggc
     3241 cacgctcaat gcagcacgcg gctcgcgccg tcatccagga cgaaggccgg aatttccgtt
     3301 tcgaaacgag cgccgtcctc cgcaacgcag aagtagcttc cctgcatggt gcccgaggcg
     3361 gtgcgcagcc ggcagccgct ggtgtactgg aaagcctcgc ccggcttgag caacggctgt
     3421 tgcccgatca cgcccaggcc cttgacttcc tcgatgtggc ccagcgcgtc cgcgatgatc
     3481 cagtgccgcg aaacaagctg ggccggcact tcacccgtgt tcgtgatcgt gatggtgtac
     3541 gcgaagctgt acaggttttg ctccggggcg gactgctcgg ggaggtaatg gggctcgact
     3601 tcgacacgga accggtactt ggccatgagg caatcgtaac ccgttggcga tttgcgagaa
     3661 tccagggatg agcacaatcc atcgcatcgc gccctccatc ctctcggccg acttcgcccg
     3721 cctgggggac gaagtggcga aagtcattgc cgccggcgcc gactggattc acttcgacgt
     3781 gatggacaac cactacgtgc ccaacctgac gttcgggccc atgatctgct cggccttgaa
     3841 gccgcacgcg gtcacggcgg cgggacacgc agttccaatc gacgttcacc tgatggtgga
     3901 gccggtggat gccctggccc aggctttcgc gcaggcgggc gcggactaca tcagctttca
     3961 cccggatgcg tcggcccacg tgcaccgcag cgtgcaggcc atcaaggcca ccggttgcaa
     4021 ggccggattg gtgttcaacc cggcgtcgtc catggatgtg ctggactgga tgatcgagga
     4081 gatcgacctc atcctcatca tgagcgtgaa ccccggtttc ggcggccagg gcttcatcga
     4141 atcggccctg cgcaagatcg agctggcgcg ccagcgcata accgattgcg gcaaggacat
     4201 ccgcctggaa gtggacggcg gcatcaagac cgacaacatc gcgcgcgtgg cggcagcggg
     4261 cgccgacacc ttcgtggccg gcagcgccat tttcggcaag cccgactaca aaggcgtgat
     4321 cgacgcgatg cgcgccgcgc tcaagggctg aacttcttca ccgggtccga ggactcctcg
     4381 cgcagctttt cgtaggtctc gtccatcacc ggccctttgt cggtgtcgac caggccccgc
     4441 tcgatgtctt cttgtgccag cttcgccatc cgcggacccg agggctcgct tgccccctgg
     4501 ctgtcggcgg attcgtcgcg ctcgtggggc atgcgcggga cccggttccg ggtatccccc
     4561 tgggccggca tcgtgttgcc ctggcgcgcg ggacccttgc ccttgctcgg gccctggcgt
     4621 cgccggctcg gaattcggtt gtgcatggcg gtgcccttcg atatgaaaca ggaagaaaga
     4681 aagcaatccg gaaagctctt cacccgatgg tgcgatgggg ccgcgtcccc agcggtagga
     4741 cgcgccgtgc cgcgcctgta ggagaaggcc ggcacgaagg ccgcccccgg ggtgtcggag
     4801 atgtcctact ctcaaatgat gtctgcgccg acaaacgcgc ccgccaccca actacgaacg
     4861 gtgcgcatga gcttgcctat gctgagggcg gcttgacgta agccggtttg gcaaggagat
     4921 cgacatggag ctgattgccg gaatgccgga atggttggtc tggtgcgccg ccggtggcgc
     4981 cggcgtcgtt gcccttgcct cactggcgaa tatcctcgac gccgcattcg agctggacgc
     5041 gagctaaggt gtactcgcgc gcccgctgcg cccttgacgg gcgcgttttc gcgccacgaa
     5101 caacaaaacc gccggggtgt tgaatgccac agtcgaagca ggatgcccag cgcaaatcga
     5161 aggccgccaa cggcgccgcc gccaagcaga aacagctgga gtcgttcagc cagggacctg
     5221 aaaacctgct caccaccaat cagggcgtgg tgattccgga caaccacaac tcgctcaagg
     5281 ccggcgtgcg cggcccatcg ctgctggaag atttcatcct gcgcgagaag atcgcgcatt
     5341 tcgaccatga gcgcattccc gagcgggtcg tctacgcgca gggcgcggcc gcgcacggct
     5401 acttcgagtt gacccgttcc ctggtcgagt acacgcgggc cgatttcctg caggaagccg
     5461 gcgcgcggac cccggttttc gtgcgcttct ccgccgcttc gggctcgcgc ggttcggccg
     5521 actcgctgcg tgattcgcgc ggcttcgcgg tgaagttcta tacccgcgag ggcaactacg
     5581 acctggtggg tggcagcatg ccggtgttct tcatccagga cgcgatcaag taccccgacc
     5641 tcatccatgc catgaagccc gagccgcacc acgggatgcc gcaagccgcc accgcgcacg
     5701 acagcttctg ggacttcgcc tcgctcacgc ccgagacgac tcacatgctg atgtggctca
     5761 tgtcggaccg ggccatcccg cgcagctacc gcatgatgga aggcttcggc gtccatacct
     5821 ttcgcctgat caacgccaag ggcgtggcgc acttcgtcaa attccactgg aaaccccgtc
     5881 tgggcaagca tgccctggta tgggacgagg cacagaagat cgcgggcaag gatcccgatt
     5941 tccaccggcg cgacctgtgg gaggcgatag tcaacggcaa ctttcccgag tgggagcttg
     6001 gcctgcagct gatcgaggag agcaaggccg acagcctggg gttcgacctg ctggacccca
     6061 ccaaactgat tccggaagaa atcgtgccgg tgctcgccgt gggcaagctg gtcttgaacc
     6121 gcaaccccga caattttttt gccgagaccg agcaggtggc gttccatccc ggccatgtgg
     6181 tgccgggcat cgacttcagc aacgatccct tgctgcaggg ccggctgttc tcatacaccg
     6241 acgcgcaact gtcgcgcctg gggggcccca atttccacga gctgccgatc aaccgaggcc
     6301 tgtgcccgtt ccacaatttt cagcgtgacg gcatgcaccg catgctggtg aacaaggggc
     6361 aggtcgctta cgagccgaac tcgttggcca gcggggccga attccgcgtc gacggcgggc
     6421 gccaggggtt ccaaagttac ccggaggcgc tcgagtcgcc gaaaacccgg cgccgcagcc
     6481 cgagcttcga cgaccacttc agccaggcca ccctgttctg gaacagccag ggcccggctg
     6541 agaaggaaca catcattgcc gcattccagt tcgagctgtc gaaagtcgag ctcgtggccg
     6601 tgcgccagcg ggtcgtggac aacctcgcgc atgtggacgc caagctggcc cgcaaggtgg
     6661 ccgagccgct gggcatcgcg ttgcccgatc cgaaggcggc ggcgggtcgc gccggctttc
     6721 gtgacatgcg caccgagctg ccgctggaaa cttccgctgc cctgagcatg gaaggcggcg
     6781 cggcgggcat cgccacgcgc agggtcgcgg tgctggttgc cgggggcgtc gaagtgggcg
     6841 ccttgcgggt catccagcag gccttgcagg acgccggagc cgccagccgc atcgtcgcgg
     6901 cccatctcgg ttttgtcgcg acatcgtcgg gccagcagct ggcggtggac cacaccttcg
     6961 cgaacatgcc gtcggtcatg ttcgacgcgg tgctggtgcc cggcggcgct gccagcgccg
     7021 aggccttggc ccgcgatggc gacgcggtgc attacgcgct ggaagcgtac aagcattgca
     7081 agaccctgtg cgtcatcggg gagagcgttc agttgctgcg cacgctcggc ataggcgcgg
     7141 gcgagggcgg ccccgcggtg gcgggcgtcc tgatcggcaa gaacgatccg acggggcgcg
     7201 cgcagctggc ccaggacttc atcgccgcga tcgcgcggca ccgccactgg atgcggccga
     7261 acctcgaagc catcccggcc tgagggtcgg gtcgcagcag cgcgacagaa gggcattcct
     7321 acgccccatt gactcaactg cttaccccat cgacccttgt gttgtcccca aatatgccgg
     7381 tagccggtgc agtgccggct gcgtgtcagt gagttcattt caggaggcca cgatggcagc
     7441 aagtaaccag gggaaccagg gcaagggcca acagggcggc tcgtccaacc ggggtttcgc
     7501 ctcgatggac ccggagcgcc aacgcgaaat cgccagcgag ggcggcaggg cggcccacca
     7561 gaagggaacc gcgcacgagt tttcctcgga agaagctcgc caggccggca gcaagggcgg
     7621 cttggccgct cacgaaaagg gcacggccca cgagttcgac tccgaggaag cccgcgaagc
     7681 cggccgcaag ggcggccagg ccagccacga aaagggcacg gcccacgagt tcgactccga
     7741 ggaagcccgt gaagccggcc gcaagggcgg ccaggccagc cgcgggggcg gcagccaggt
     7801 tggagccaac cagggcggcg accagggcgg ctcgagcggc aagcgctgaa agcggtccgc
     7861 ggcatcaagg gcgctgtgtt cgggcgccct tttttgttgg gagtgaccat cgtcgtccgg
     7921 cagccaccgg atgcgccaga atccgcgaac cttcttcctt tttttccgat cctcgttatg
     7981 acccccatcg ctgcacaagc ggccatcgtc gacctcgacg gcacgatggt cgacacgctg
     8041 ggcgactttg ccgtggcttt gaaccgggtc ctggacgaac tgtcgctgcc cgggatcgag
     8101 aggtccgaga tcgaacgcat ggtgggcaag ggttcgccgc acctgatccg ctcgatgctg
     8161 gcgcatgtcc gggccgcgcc ctccctgtac gagcaggcgt ggcagtccta ccggaagcat
     8221 tacctggcca tcaacggcca atacgcttcg gtctatcccg gcgtgaccga aggtctgcgc
     8281 acgctcaagg ccgccggcct gaagctcgcg tgcctgacca acaagccgat cgcgtttgcc
     8341 ggccctttgc tggaggcgaa gggcctgcgt gaatacttcc agctggtgtt cggcggcgaa
     8401 gcgttcgagc gccagaagcc ggatcccctg ccgttgctca agacctgcga agcgctgggt
     8461 tcagcgcccg ctcgcacgct cgtgatcggc gattcgagca acgacgcccg tgcggcccgc
     8521 gccgccggtt gcccggtgtt gctggtgacc tacggctaca accacgggga gcccgtgcgc
     8581 gccgtggacg ccgacggttt tctggattcg ctggcggaag tgccgggctg gctcaggcgg
     8641 ccttacccaa cagcgcttcc ttgagcttcc agtcggcggg attgggcccc agccagatgc
     8701 gcaggagcgc attgaagaat tccggttcct tgaagggttc gccctgtggc acacctttaa
     8761 cggtgagtag cgtgccggtg ccggggaccc attcaagcga caaagtgtcg ccgggaccga
     8821 gcttcttgtg atcggagaac acctggctca tgcgcagcac gccgggaatc agcttggaga
     8881 aagccgcctt ttccatgttg tcttccatgc cacgggaaaa catcttgccg aactccgacg
     8941 aatcgatctc gcgcagcatg gtgatgttca ggcgcttggc gcccggcatc gccagcacct
     9001 gttcgggagt gccgcccttc gttggcaggt aaagccccgc cgcgtaaacc ttgaagatgg
     9061 ccttgtaccg cgtgcctgcg ccattgagct ggagcttggt gccgcgcaca tcggccgact
     9121 ggtcgaactt gacccccgat accgtcacct gggcgaaagc ggccgaagcc agaagaaatg
     9181 tggccgtcgc gaacagccaa cgcttgagca agctcatatg gttttcctaa aaagtacgat
     9241 cgttcgattt tcggagacca aggctgagtt cgcaataggg ttttcgcgta accgaatgtt
     9301 aataactccg attcgccgag ggaaaccggc tcgctttgct acactttcac ccatgttcat
     9361 ccaccgcacc acgatcacag ggcatccggg ccggggagcc tggccctggc gtaagcctca
     9421 atcggtgcgt tcctgaccgc acggggcagc ccgtgccccg cgcgcctcga aactgtcggg
     9481 ccgccgcgcc tgatcccaga ggcatcatgt tgaattgaaa accggccgcg aggccgccga
     9541 gcttgtggag gctcaccctt gatcaccgaa ctcgaattca aaagccttgg cttgcagggc
     9601 tacaaccgca tcccgctgat cgccgaggcg ttcgcggacc tcgaaacgcc cctgtcgctc
     9661 tatctgaagc tcgctcacgc caagggcggc ggcaagcaca gcttcctgct ggagtcggtg
     9721 gtgggcggtg agcggttcgg gcgctacagc ttcatcggcc tgcccgcgcg aactttgctg
     9781 cgttgcagcg gcttcggcgc cgacgccgtc acgcaggtgg tcgaggacgg ccaggtggtg
     9841 gaaacggccc gcggcaatcc gctggatttc atcgccgaat accagaagcg cttcaaggtc
     9901 gccctgcgtc cgggcctgcc gcgcttttgc ggtggactgg ccggctactt cggctacgac
     9961 acggtgcgct acatcgagaa aaagctggag aacagctgcc cgagcgatac gctgggctgc
    10021 cccgatatcc tgctgctgca gtgcgaggag ctggccgtca tcgacaacct gtcgggcaaa
    10081 ctctatctca tcgtgtacgc cgatccgggc cagcccgagg cctacgccaa cggcaagaag
    10141 cggctgcgcg aactcaggga gcagctcaaa tattcggtga gcgcaccggt gatccggcaa
    10201 acccagacct tcgccgccga acgcgagttc gcgaaggccg acttcctcaa ggcggtggaa
    10261 cgcgccaagg agctgatcgc cgcgggcgac ttcatgcagg tgcaggtcgg ccagcgcatc
    10321 aagaagcgct acaccgagtc gccgctgtcg ctgtaccgcg cgctgcgctc gctcaacccc
    10381 agcccctaca tgtactacta ccacttcggc gattttcacg tggtgggggc ctcgccggag
    10441 atcctggtgc gccaggagca gacgcccgaa ggccagaagg tcacgatccg gccgctggcg
    10501 ggaacccggc cgcgcgccac cacgccggaa ggcgacaagg ccatcgagca ggagctgatc
    10561 aacgatccca aggagcgcgc cgagcacgtg atgctgatcg acctggcgcg caacgacatc
    10621 ggccgcatcg ccaagaccgg cacggtgaag gtgaccgagg cattcgcggt ggagcgttac
    10681 agccatgtga tgcacatcgt cagcaacgtg gagggcatcc tcaatgacgg gatgaccagc
    10741 atcgacgtgc tgaaggcgac attcccggcc ggcaccctga ccggcgctcc caaggtgcac
    10801 gcgatggagc tgatcgacca gctcgaaccg accaaacgcg gcctctacgg cggtgcctgc
    10861 ggctatctca gctacgcagg cgacatggac gtggcgatcg cgattcgcac cggcatcgtc
    10921 aaggaccaga cgctctatgt gcaggccgcc gccggcatcg tcgcggattc ggtgcccgag
    10981 ctggagtgga aggaaaccga agccaaggcg cgcgcgttgc tgcgggcgtc ggaattggtc
    11041 gaggagggcc tggaatgagc gccgcgcaga accgcttcaa gcaagctcgc gcccccttgg
    11101 ggggcagtgc agcgggcctg tccgcaaacg tgggggccct atgcgtctgc tgatgatcga
    11161 caactacgac agcttcacct acaacctggt gcagtacttc ggcgagctgg gcgcccaggt
    11221 ggaggtgttt cgcaacgacg agatcacggt ggggggcatc gcgcagcgca agcccgatcg
    11281 gctggtcgtc tcgcccggcc cctgcacgcc ggccgaagcc gggatttcgg tcgaggcgat
    11341 ccgccacttc gccggcaagc tgccgatcct gggcgtgtgc ctgggtcacc aggccgtggg
    11401 agcggcattc ggcggcaaca tcgtccgtgc ccggcagctc atgcatggca agaccagcgt
    11461 gatcacgacg accggcgaag gggttttcgc cggcctgccg gaaaagttca ccgtcaaccg
    11521 ctatcactcg ctggccatcg aaaagtcatc ctgcccgggc gaactcgcta ccacggcctg
    11581 gaccgatgac ggcgagatca tgggcgtgcg gcacaagcag ctggcgatcg agggcgtgca
    11641 gttccatccc gaatcgatcc tgaccgagca tggccacgcc atgctcagga acttcctgga
    11701 gcaaagcaaa tgagccccca cgttcacatt cgttctctgc cccccgaggg gtcgcatgcc
    11761 gccctagagg cggctcgtcg ggaggcatga tggcaacgat cgtggacctg cgcagcgaca
    11821 cggtcacccg gccgacaccc ggcatgcgag ccgctatgga cagcgcgccg ctcggcgacg
    11881 acgtgttcgg ggacgatccg agcgtgaacg cgctgcagga caagatcgcc ggaatgctgg
    11941 gattcgaggc ggcgctgttc gtgccgaccg ggacgcaaag caacctgtgc gcgatcctgt
    12001 cgcactgcca gcgcggcgac gagtacatcg tcggccagat gcagcactgc taccgctggg
    12061 aagcgggcgg cggcgcggtg ttcggaagcg tgcagcccca gccgctggag caccgggccg
    12121 acggcagcct ggccctggcc gacatcgcgg cggcgatcaa gccggacgac ccgcatttcg
    12181 cgcgcacccg gatgctggcg ctggagaaca cgctgggcgg caagctgctg ccgttcgcct
    12241 ttatcgagca ggcgaccgaa ctggcgcagc gcagcggcct ggcgcgccac ctggacggcg
    12301 cgcgcctctt caacgccgcc gtggcccagg cggcgcaaac gggcggcgac gcgcgtgccg
    12361 aggcgcggcg catcgcgcag cgtttcgaca gcgtctcggt gtgcttcagc aagggattgg
    12421 gagcgcccgt cggctctgcc ctgtgcggtt cacgcgagct gatcgcgcgt gcgcggcgcg
    12481 tgcgcaagat ggccggcggg gccatgcgcc aggccggcat cctcgcggcg gcggcgagct
    12541 atgcgctgga tcatcatgtg gaccggctgg cccaggacca tgcgctggcg ctgcggctgg
    12601 cccaggggct gggcgacatc gagaacctgc gcgtcgaggc gccgcagacc aatatggtct
    12661 tcatggacct ggtggggccg gcccgggagg agtccaaggc attgctcaag cacctggcaa
    12721 gccatggcat cctggcgacc gggctgtacc ggctgcgatt cgcgacccac ctcgacctgg
    12781 atgccaacgg ggtggaccgg gccgtcgccg cgatccggca gtatttcggg aagtgaccgc
    12841 cgtgcccgat ccaagctcgc tttgaccgac aatcccacac gctaaggaaa accgtcatgc
    12901 ccatcactcc ccaggaagcg ctgcagcgca cgatcgaaca ccgtgagatc ttccatgacg
    12961 agatgctcaa ggtggttcgc atgatcatga gtggagagct gtcgcccgtg atgatggcgg
    13021 cgctgatcac cggattgcgc gtcaagaagg aaaccatcgg cgagatcacc gccgcggccc
    13081 aggtcatgcg cgagttctcg accaaggtca acgtggccga caagacaaat ctggtcgaca
    13141 tcgtgggcac cggcggcgac ggctcgcaca ccttcaacat ctccacctgc tccatgttcg
    13201 tggcggccgc ggcgggcgcc aaggtcagca agcacggggg acgcagcgtg tccagcaaga
    13261 gcggcagcgc cgacgtgctg gaaagcctgg ggatgaacat caatctgccg cccgatgcca
    13321 tcgccagatg cattgccgag gtcggcatcg gcttcatgtt cgcgcccaac caccacccgg
    13381 cgatgaagaa cgtggcgccg gtgcgcaagg aactgggcgt gcgcaccatc ttcaacatcc
    13441 tgggcccgct taccaacccg gccggcgcgc ccaacatcct gatgggcgtg ttccaccccg
    13501 acctcgtggg catccaggtg cgcgcgctgc aacgactggg gaccgatcat gcgctggtgg
    13561 tctatgggcg cgacggcatg gacgaggtga gcctgggcgc ggcgacgatg gtcggcgaac
    13621 tcaagaacgg cgagatctcc gagtacgaaa ttcatccgga ggatttcggc ctgccgatgg
    13681 ccagcaatcg cacgctcaag gtcgaaacac ccgaacagtc gcgccagatg ctgaccggtg
    13741 tgctcgacaa cgaggccggc gctgcgcgcg acatcgtgat cctgaacgcc ggcgccgcgc
    13801 tctatgccgc caacgtggcg gacagcatcc aggcgggcat cggcaaggcg cgggaggcgc
    13861 tcgcttcggg tgccgcgaag gccaagctgg aacagctggt gaagctttcc caaacgctcg
    13921 cagcctgagc catgccggac atcctggacc ggatcgtagc ggtcaagcgc gaggagatcg
    13981 ccgccgcgca ggaaaaagtg ccctttgcgg ccatgcgcat ggacgcggaa agccgcgtcc
    14041 tgacgcgcga tttcgaagga gccctgcggc gcaagatcgc ggccggcaag agcgccgtga
    14101 tcgcggagat caagaaggcg agcccgagca agggcgtcct gcgggccgaa ttcatcccgg
    14161 ccgacatcgc gcaaagctat gccgagtccg gggcggcctg cctgtcggtt ctgaccgacc
    14221 ggcagttctt ccagggccag gtcgatgacc tgaagcaggc ccgggcctcc tgcgacctgc
    14281 cggtgctgcg caaggacttc atggtcgacg cctaccagat ctaccagtcg cgggcgatcg
    14341 gcgccgactg cattctgctc atcgcggcct gcctggagga tgcccggatg gcggaactcg
    14401 aggccgtcgc ccgcagcctg gacatggccg tgctggtcga agtgcacgac cggtccgaac
    14461 tggagcgcgc actcaagctc aagacgccgc tggtgggcat caacaaccgc aatctgcaca
    14521 ccttcgaagt caccctggac gccacgctgg acctggtcaa ggaagtaccg gccgaccgcc
    14581 tgctggtcac cgaatccggc atcgtgacca aggccgatgt gaacaagatg cgcgcggccg
    14641 gcgtgcacgc cttcctggtg ggcgaagctt tcatgcgcgc cgacgatccg ggcgaggccc
    14701 tggccgcgct gttcggcgac tgatcggttc ggcggcgatg gcagcgcgac ccggtccggc
    14761 cgaacaagcc tcgcaagcgg agctcgagct cgagatgcgt gagcccgacc gcctgttgcg
    14821 atgggagccc gagccctggc agctggccga gggctggcgc gacatcgtcg aggccttcct
    14881 gtcgagcacg gccggcttgc ggctggcttc atacatccgc gagcgtctgg ccgccggcgc
    14941 cacgatctat ccgccgcggc cgtttcgcgc gctggagctc acaccgctgg acagcgtccg
    15001 ggcggtaatt ctcggccagg atccctacca tgggccgggc caggccgagg ggctcgcgtt
    15061 ctcggtccgg gcgggcgtca agctgccgcc gtccctgcgc aacatcttca aggaactgca
    15121 tggcgccgac gacgaatcgc agcccccgac cggctcgctg gtcgactggg cccggcgcgg
    15181 cgtgctgctg ctcaatgcca gcctcacggt ggaggacgga cagccgggaa gccatgcccg
    15241 caaaggttgg gaagtgctga ccgatgcctt gctggccgag gtcgcggccc gcgcatcgcc
    15301 ttgcgtctac atgctctggg gcgcccatgc ccaggccaag gcagcgctga tcgagaatac
    15361 cgccgcgcgc cacggctgcg aggctctggt gctgcaggcc aaccatcctt cgcccctgtc
    15421 ggccggacgc ccaccggtgc cgttcctcgg ctgcggccat ttcgcgctgg cccgtgactg
    15481 gctggcggcg cgcggctatc cgggtgtgtt ctagggcctg ttcacaccct agttccggac
    15541 ttgcccgacc cgccaggagc ctctgaacag ccgagccagc catggcctga agccgcgctg
    15601 cggccggccg gggccctgcg gtctcagcgt ttcctcgcgc gtgtcgtcca ggtaatcgcg
    15661 ccggatgtcg atcgtgctgc gccggccaag cgccgcgaaa tgccgggcca gccactgggt
    15721 ggcgctggcc tggccgagct cgaacagctt gccgatcatg acggcgtcgg tcgaggtccg
    15781 gctggacgcg ggatagtgcg ccatgtcggc tccgccgtcg atccggtgca gcagcacatg
    15841 cttgcaccgt cccccgcgca acgcgccttc ctcgatcagt cgattgatga agtcgatcga
    15901 ccgcatctgg gtcagcagac tggcgttgaa ggtcaattcg ttgatccggt ccaggatttc
    15961 cgtgggcgat tgcggcaccc gttcgcgctt gagcgggttg atctgcacca gcagcaggtc
    16021 gcggctttcg cacgcttcga tcagcggagc caacggggga ttgaccgaat agccgccatc
    16081 ccagtagttt tcgccgtcga tcggaaccgc tcgaaacagc atcggcaggc aggccgaggc
    16141 catcacggcc tgcacagtga ggcgctggcc cgtgaagagg accgctcgcc cggtgttcac
    16201 atgggtggcc gaaacgaaaa ccttgagttg ccgggacttc gcgatcgcgt cgaagtcaat
    16261 ttcgcgctcg agcaggctgc gcagcgggtt gatgtccagc gggttggact gatagggtga
    16321 aagcaggctg cgccaggcct ttgccatgat gttagctggc gccaactccg gcggcagacc
    16381 cccccagagc atcccgacca tgcgctgctg catggcgccc agggtgcccc agtccgagat
    16441 ctcgtgccag acgcgtccca gcgactcgcg ggccgattgg cgcggatcgt ggccggccgc
    16501 aaggcctttg gcgtagccgc tggccaaggc caccgcattg acggcgccgg cgctggcgcc
    16561 gctcaggccc tcgatcgcga tccggtcgtc cgccagcagc gcgtccagcg cgccccatgt
    16621 gaaggcaccg tgcgagccgc cgccttgcaa ggccagattg agggtccgca tggcagaagt
    16681 gcaagcaaca ttcatgcggg ccattgtgag tgcggctggt gtcggttccg ggtaggcgcg
    16741 gttcccgtta cacacccggc aggtgcggcg ttaaccagcc ccgtccggga ggtccgcgag
    16801 ttcgaaacgt gctatatttc gaggttcgcc ggaggggtgc ccgagtggct aaagggggca
    16861 gactgtaaat ctgttggctt acgcctacga tggttcgaat ccatcctcct ccaccaggcc
    16921 tcgagcgagg ctcggcgaag ttgtcgaagt tgatttgtgc agagcgcggt tttggagcga
    16981 atcctgcggg gttttcgtgg aaatcccaag gggttcgagg cctgctgggg tcgcctgaag
    17041 ttcacccgat gcgggagtag ttcaatggta gaaccttagc cttccaagct aatgacgcgg
    17101 gttcgattcc cgtctcccgc tccatgaacc ggctcggcgt gcgaaaaaga agaattcgcc
    17161 cttgtggctc agtggtagag cactcccttg gtaagggaga ggccgcgggt ccgattcccg
    17221 ccaagggcac cagatcaggg tgcacgtttt tcgaagtgca ccgcggttga gttgagttgg
    17281 gaatcgtcta gttacttttc ggagtcgaaa aatggcaaaa gcaaaattcg agcggaccaa
    17341 gccgcacgtg aacgtgggca cgatcggcca cgtggaccat ggcaagacca cgctgacggc
    17401 ggccatcacc tcggtgctgg cggccaagtt cggcggcgaa gtcaagg
//
LOCUS       DASWIY010000335         5186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1324477, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000335 DASWIY010000000
VERSION     DASWIY010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324477"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="VGA59_12010"
     CDS             complement(<1..1579)
                     /locus_tag="VGA59_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9720446.1"
                     /translation="MKLPRILTLFVAAVACHVQAQPMADDLIVEMNRAFQQGNQSKLS
                     QLLPQVRGHALEPWAAYWELKTRLDTALPPEVQDFFTRYAGTYQEDRLRNDWLLLLGQ
                     RRDWTNFDAEYPKFRMKDDREVRCYSILVALTREGAAAPAALADEVRHNWFSLRDVDD
                     GCTLAASRLVGNTEGPRMSPNDAWHKARLAMAASRPDAAAAAVRIVAPEAFAQFTELN
                     NSPARFLVRRVVAASKVRKEMIVLALIKLATGDADNAARQLDGKWGPQLTPEERNWVW
                     GVIARQKAQRLGDDALEHYAKVSRDNDLTDDMLAWKVRAALRAARGPQWAAVLQATNA
                     MSEEARREPAWVYWKARALLAGNPADTRRAEAPRLLESIASPRGFYEQLALEELGRKI
                     TVPPRPTPLTAEEKDAVRANPGLNRALQAIALGIRPEGVREWNYTTNVAKPGGMAERE
                     LLAAAQLACEREVWDRCINTSERTKTAFDFEQRFPMPFRESVVKRSQEIGLDPAYVYG
                     LIRQESRFIMDARSNVGA"
     gene            1589..2176
                     /locus_tag="VGA59_12015"
     CDS             1589..2176
                     /locus_tag="VGA59_12015"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX9720447.1"
                     /translation="MDRSEEKSSLRKQLIERRLNMPDRIRRCDMLQRVMRIWLVNRPD
                     TVIGAYWPIKGEFDPLPALHRWKEDGELLEEPQPRRIGLPVIDKLHRTLKFHAWFPGC
                     PMEEDAFGIPKPKDTEIIVPTLLFVPCVGYGLGGHRLGYGGGFYDRTLSSLQPRPYTV
                     GLGFREGFLGDLEPEPHDVALDAILNDNGVVWPIS"
     gene            complement(2283..3266)
                     /locus_tag="VGA59_12020"
     CDS             complement(2283..3266)
                     /locus_tag="VGA59_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9720448.1"
                     /translation="MQHSQTHLRTRPISAGHLRAFEAVARHLNFRAASEEMSLTQSAV
                     SRQIQALEEEVGASLFLRHTRAVELTGAGAQLLLAVTQSLPRIDSAVRQIRQSAGRRT
                     VALTTFASFASMWMIPRLEQFQRDHPDIDIRIDASDTTADLDVADVDLALRYGPMATM
                     PSHATRLFGEQLTPVVSPWLLKSGAPLKKPADLAQFALIEAGDAQHTHYEWLTWRRWF
                     DEQGLPKLQPKRWLYFNYAYQMVQAALTGQGVVLARLPLVAESLANGDLVEPLPQQRM
                     DSPMAYWLLVGPRSAQRPEIKAFCEWLMAQSKATRHTIGEVPDPDTIDDLD"
     gene            3400..3903
                     /locus_tag="VGA59_12025"
     CDS             3400..3903
                     /locus_tag="VGA59_12025"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEX9720449.1"
                     /translation="MASQTLTQMQQSSSAPALPGTWKLAARRAITLQPREAGLLRVAH
                     GQLWVTFDGPHDGPRNDLGDHVIGVGEQLRLRAGQRLVVEAWDPRSASYFSWDPLPAG
                     VRGAAPRFAAVVQPLADLRLAFMFGADAAGRLVAGLAALAWDWLPRAKDAKLSSGERS
                     NPELNCT"
     gene            3959..4558
                     /locus_tag="VGA59_12030"
     CDS             3959..4558
                     /locus_tag="VGA59_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9720450.1"
                     /translation="MARPKSSQHELKRDEILDVAAQCFADRSYPAASMNDIAAAGGTS
                     KARLYHYYESKEAILFDLLDRYTQRLLGVIGQVEATAQRRSLDDRATLHELVRSFLEE
                     YEHSATRHIALLHDTKFLGDAQRDLILNRQRDVVSAVTRFLRRAYPGRVNAANQTPVT
                     MMLFGMINWTFTWLRPGGPLSYSAFAEDVIAMLEHGLAQ"
     gene            4555..5061
                     /locus_tag="VGA59_12035"
     CDS             4555..5061
                     /locus_tag="VGA59_12035"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX9720451.1"
                     /translation="MTAASHVVVMGVAGCGKTAVGRRIAQLRGLPLIEGDEFHPEGNI
                     DKMRQGLALDDQDRAGWLKRLGGELMRHPDGAVLTCSALKRSYRDSLREAAPGLRFVH
                     LSLSEEQALQRVAARDDHFYPPILVASQFEALEDPRSEPGVLVVDALLPVEELACRAA
                     DWLAEPGP"
BASE COUNT          845 a         1701 c         1740 g          900 t
ORIGIN      
        1 aggcgcccac gttggaacgc gcatccatga tgaagcggct ttcctggcgg atcaatccgt
       61 acacgtaggc cgggtcgagc ccgatctcct ggctgcgctt gaccaccgat tcgcggaacg
      121 gcatggggaa gcgctgctcg aagtcgaacg cggttttcgt tcgttcgctg gtgttgatgc
      181 agcggtccca gacctcgcgc tcgcaggcca gctgcgcggc cgccagcaac tcgcgctcgg
      241 ccatgccgcc gggcttggcc acgttggtgg tgtagttcca ttcacgcacg ccttccggcc
      301 ggatgcccag ggcgatcgcc tgcaaagcgc ggttcaggcc gggattggcg cgcaccgcgt
      361 ccttttcttc ggccgtcagc ggcgtcggcc gcggcggcac cgtgattttc cggcccagct
      421 cctccagcgc aagctgttcg tagaaaccgc gcggcgaggc gatcgactcg agcaaccgtg
      481 gcgcttcggc gcggcgagtg tcggccgggt tgccggcgag cagggcccgt gccttccagt
      541 agacccaggc gggctcgcgg cgcgcctcct cggacatcgc attggtcgcc tgcagcacgg
      601 ccgcccattg cggtccgcgc gcggcgcgca aggcggcacg caccttccag gccagcatgt
      661 catcggtgag gtcgttatcg cggctgacct tggcgtagtg ctcgagggcg tcatcgccca
      721 ggcgttgcgc cttctgcctg gcgatcaccc cccacaccca gttgcgctcc tcgggcgtga
      781 gctgtggacc ccatttgcca tcgagctggc gcgcggcgtt gtcggcatcg ccggtggcga
      841 gcttgatcag tgccagcacg atcatttcct tgcgcacctt cgatgcggcc accacgcgac
      901 ggaccaggaa cctggccgga ctgttgttga gttcagtgaa ctgggcgaac gcctcgggcg
      961 ccacgatccg gaccgcggcg gcggcggcgt ctggccggct cgccgccatg gcaagccgcg
     1021 ccttgtgcca ggcgtcgttg ggggacatcc gcggcccctc ggtattgcca accaggcgag
     1081 aggccgcgag cgtgcagcca tcgtcgacat cacgcaaaga gaaccagttg tgccttacct
     1141 cgtccgccag ggcggccggc gcggcggccc cctcgcgcgt gagcgcgacc aatatcgagt
     1201 agcagcgcac ctcgcggtcg tctttcatgc ggaacttggg atactcggca tcgaaattgg
     1261 tccagtcgcg gcgctggccc agcagcagca gccagtcgtt gcgaagtcgg tcctcctggt
     1321 aggtgcccgc atagcgcgtg aaaaagtcct ggacttccgg cggcaacgcc gtgtccaggc
     1381 gggttttcag ctcccagtag gcggcccacg gttcgagcgc gtgaccgcgc acctgaggca
     1441 gcagctgaga cagcttggac tggttgccct gctggaacgc ccggttcatt tccacgatca
     1501 gatcgtcggc catgggctgg gcctggacgt ggcaggcgac ggcagcgacg aacagtgtca
     1561 gaattcttgg caacttcatt ggcgaattat ggacaggtca gaagaaaaaa gctctttgcg
     1621 caagcagctg atcgagcggc gcctgaacat gccggaccgc attcggcgct gcgatatgct
     1681 gcagcgggtc atgcgcatct ggctggtcaa ccgcccggac acggtgatcg gcgcgtactg
     1741 gcccatcaag ggcgagttcg atccgctgcc ggccctgcat cgctggaagg aagacggtga
     1801 actgctggag gaaccacagc cgcggcgcat cggcttgccg gtcatcgaca agctccatag
     1861 gacgctgaag ttccacgcat ggttccccgg ctgcccgatg gaagaggacg ctttcggcat
     1921 tcccaagccc aaggacaccg aaatcatcgt gcccaccttg ctgttcgtcc cgtgcgtggg
     1981 ctacggcctc ggaggccacc ggctcgggta cggcggcggt ttctacgacc gcacgctgtc
     2041 gtcgctgcag ccccggccct acaccgtcgg cctggggttc agggagggct tcctgggtga
     2101 cctcgaaccc gagccgcacg acgtggcatt ggacgccatc ctgaacgaca acggcgtcgt
     2161 gtggccgatc agctgaatcc tcgtttgtca tcccggaccc agctgtcatc ccgggcttga
     2221 cccgggatcc atgacttggc ttcgatggat tgcgggtcaa gcccgcaatg accacaaagc
     2281 tcctaatcga ggtcgtcgat ggtatcgggg tccggcacct cgccgatggt gtggcgcgtg
     2341 gccttgctct gggccatgag ccattcgcag aacgccttga tctccggccg ctgtgcgctg
     2401 cgcgggccga cgagcagcca gtaagccatc ggcgaatcca tgcgctgttg cggcagcggc
     2461 tccaccaggt cgccattggc caggctttcg gccaccagcg gcagccgcgc cagcacgacg
     2521 ccctgtccgg tcagtgccgc ctgcaccatc tggtaggcat agttgaaata cagccagcgc
     2581 ttgggctgca gcttgggcag gccttgctcg tcgaaccagc ggcgccacgt cagccattcg
     2641 tagtgggtat gctgcgcgtc gcccgcctcg atcaaggcga actgggccag gtcggccggc
     2701 ttcttcagcg gcgcgccgct cttgaggagc cacgggctga ccacgggcgt gagttgctcc
     2761 ccgaacaggc gggtcgcatg cgacggcatc gtcgccatgg ggccgtagcg cagcgccaga
     2821 tcgacatcgg cgacgtcaag gtccgcggtg gtgtcggatg catcgatccg gatatcgatg
     2881 tcggggtggt cccgctggaa ctgctcgagc cggggaatca tccacatcga tgcgaacgac
     2941 gcgaacgtgg tcaaggccac cgttctgcgg ccggcgctct ggcggatctg ccgcaccgcg
     3001 ctgtcgatgc gcggcagcga ttgcgtcacc gccagcagca gctgggcgcc ggcgccggtc
     3061 agctccaccg cccgcgtgtg gcgcaggaac aggctggctc cgacctcctc ttccagtgcc
     3121 tggatctggc ggctcaccgc cgactgagtg agcgacatct cctcggaagc ggcgcggaag
     3181 ttcaggtgcc gggcgacggc ttcgaaggcg cgcagatggc cggccgaaat cgggcgggtg
     3241 cgcaggtgtg tctgggagtg ctgcatggtg ccggtttttg tcaggagaga ggcctgcatt
     3301 gatgcgaatc gggaatcaat agcgtagccg gatttcattg gactgccaag cgcctgtcgc
     3361 cgatcattca ctgccgaatc gcataaatgg agaaatgtca tggcctcgca aacgctgacg
     3421 caaatgcaac aatcttcttc cgctcccgcg ctccccggga cctggaagct ggctgcccgc
     3481 cgcgccatca ccctccagcc gcgcgaggcc ggcctgctgc gcgtggccca cgggcagctc
     3541 tgggtcacgt tcgatggtcc gcacgatggg ccgcgcaacg acctgggaga ccacgtcatc
     3601 ggcgtgggcg aacagctgcg gctgcgcgcc ggacagcggc tggtggtcga ggcctgggac
     3661 ccgcgctcgg cctcgtactt cagctgggat ccgctgccgg ccggggttcg cggcgcggcg
     3721 ccccgattcg cagccgttgt ccagcccctg gccgacttgc gcctggcttt catgttcggc
     3781 gcggacgcgg ccggccggct ggtcgcaggc ctggccgcgc tggcctggga ttggcttcca
     3841 cgcgcgaagg acgcgaagct ttccagcggg gagcgaagca atccggaact gaattgcacc
     3901 tgaatccgcg ggtcccgcgg gcacggaagt aagtcgttcg tgcacgcata attggtgcat
     3961 ggcccggccc aagtcatcgc agcacgaact caaacgggac gaaatcctgg atgtggcggc
     4021 ccagtgcttc gccgaccgca gctacccggc ggcgagcatg aacgatatcg ccgcggccgg
     4081 cggcacgtcg aaggcgcggc tgtaccacta ttacgaaagc aaggaagcga tcctgttcga
     4141 cctgctggat cgctacaccc agcgcctgct cggcgtgatc ggccaggtgg aagcgacggc
     4201 gcagcgccgc agcctggacg acagggccac cctgcatgag ctggtccgca gcttcctgga
     4261 agaatacgag cattccgcaa cccgccatat cgcgttgctg catgacacca agttcctggg
     4321 tgacgcgcag cgcgacctga tcctgaaccg gcaacgcgac gtggtctcgg cggtcacccg
     4381 gttcctgcgc cgcgcctacc cgggccgggt caacgcagcc aaccagacgc ccgtcaccat
     4441 gatgctgttc ggcatgatca actggacttt cacctggctg cgccccggcg ggccgctgag
     4501 ctactcggcc ttcgctgaag atgtcatcgc gatgctcgag cacgggctgg cgcaatgacg
     4561 gcggcctcgc acgtcgtggt gatgggcgtt gccggatgcg gcaagacggc ggtgggccgg
     4621 cgaatcgcgc agctgcgggg gctgccgctg atcgagggcg acgagttcca tcctgaaggc
     4681 aatatcgaca agatgcgcca ggggcttgcg ctggatgacc aggaccgggc cggctggctg
     4741 aaacgcctgg gcggcgaact gatgcgtcac ccggacggcg ccgtgctgac ctgttcggcg
     4801 ctcaagcgct cctaccgcga cagcttgcgc gaggccgccc ctgggctgcg ttttgtccac
     4861 ctcagccttt ccgaggagca ggccttgcag cgcgtggccg cccgcgacga ccatttctat
     4921 ccgccaatac tggtggccag ccagttcgaa gcgctggaag acccgcggtc cgaacccggc
     4981 gtgctggtgg tcgatgcgct cctgccggtg gaggaactgg cgtgtcgggc ggcggactgg
     5041 cttgccgagc cggggccttg aagattcaac aatgcgtaac tgattacgct tgggtaagat
     5101 catcggcttc ccgtttttgc gtccgcgcaa aggagagccg agatgtccaa agcctttgcc
     5161 agccaggccg acctggccga caagaa
//
LOCUS       DASWIY010000336         9007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_963781, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000336 DASWIY010000000
VERSION     DASWIY010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9007
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963781"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VGA59_12040"
     CDS             complement(<1..494)
                     /locus_tag="VGA59_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720452.1"
                     /translation="MKTHLRNYVTSAMLLLPATVSLVALPSAALAQPATPEVRSLAVQ
                     ADAGLEPGSRLRFRLVGTPRVKASVRIRGVRENIALRETAPGVYVGRYTLKRADRVGP
                     DSDVRAMLRRGNRTIVANYELGQALGTPPVAVVPPPPVRAADPLRIERFGMVPIERIE
                     PGAEL"
     gene            complement(661..1587)
                     /locus_tag="VGA59_12045"
     CDS             complement(661..1587)
                     /locus_tag="VGA59_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9720453.1"
                     /translation="MSRSLQGKVAIVTGAGGGLGRSHAIELAKHGARVVVNDLGADAE
                     GSGARAVADEIRRCGGEAIVNGGDVTSFSAMEGMVEQAIQAWGRVDVLVNNAGILRDK
                     SFAKMTLDDFRLVIDVHLMGSVNCTKAVWSLMREQNYGRIVMTTSSSGLYGNFGQANY
                     GAAKMALVGLMQTLALEGERNNVRVNCLAPTAATAMTEGILQQDVLARLHPSAVSAGL
                     VALVSDDAPTRTILMSGAGSFECAHITMTQGIHVGHSEDATAQVRARFDEIRDRTGEI
                     VPRTGFEQAHHELTKGGMCADGMSANGRTVMA"
     gene            complement(1765..2721)
                     /locus_tag="VGA59_12050"
     CDS             complement(1765..2721)
                     /locus_tag="VGA59_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9720454.1"
                     /translation="MSLWRRHAWIAYLAVFIGVLGHASTEFFAVLSGVKGPELSVWRF
                     LLGGFALVLLALCFPSSRNLVAPMRTHGWPLLGLSVLGITFGYLVFHWALDFATVAQV
                     ATVVTTIPIFVGISNLLINKKQFTTAKIVSGTCAVIGITLLITDGYLGALAGSSANVF
                     GIVLAILCAAFVAAYSVMVRPIIEQYGALRITAITMGIGAIGLWLVVGVFFGVWVSPE
                     RLTAMESTALLSLLTIAFWNTTITQFLWIGGLAAVPDITRGSYLFFLKPVIAALLAVI
                     ILGQSLTLLQILAIVVITASVLGEIILSQPKSTAPKSAASTG"
     gene            complement(2723..3541)
                     /locus_tag="VGA59_12055"
     CDS             complement(2723..3541)
                     /locus_tag="VGA59_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9720455.1"
                     /translation="MPTFEIDSDNALFYLHTPPAAPEGMTFVFFNALTGDTGMWEGAI
                     GSCLRQAGHGTLSFNFRGQTGSPFAPGTRLDATRIVEDARRLLTALAPQNIVLTGLSI
                     GGLFAAQAWLAGLGGLKVRGLVLINTLRSDGPRLRWVNDALVRCAEVGGLTLFRDLYA
                     PLLFNQDWLAANRANFLKPGPYQPLAPDSGHYQLLANAGSADWDLPYEDLALPVLVIT
                     GLQDHVFLDQDDVNRLFARLPEARRLNMADAAHLLPAERPEALASALLDFAREC"
     gene            complement(3751..4662)
                     /locus_tag="VGA59_12060"
     CDS             complement(3751..4662)
                     /locus_tag="VGA59_12060"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX9720456.1"
                     /translation="MTKPFKVAVMGAGAVGCYYGGMLARAGHDVVLIARPPHVEAIER
                     DGLRMETRTFDEHVRLAASTEPGAVQGANLVLFCVKSTDTEAAGAQMRPHLAPEALVL
                     TLQNGVDNADRLRTVLPPHQVSAAVVYVATEMAGPGHVRHHGRGELVIEPSGAGDAVA
                     RALIAAGVPTDISDNVRGALWTKLVINCAYNAVSAIAQRPYGEAVAGEGVKDVMREVV
                     DECLAVARAEGVQLPGDVHAAVGKIVETMPGQYSSTAQDLARGKRSEIDYLNGLIVRR
                     GQALGIATPANRVLWALVKLMEGKQAN"
     gene            4735..5202
                     /locus_tag="VGA59_12065"
     CDS             4735..5202
                     /locus_tag="VGA59_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX9720457.1"
                     /translation="MNYWLMKSEPAECSVDDALAAPNATVPWVGVRNYQARNFMRDRM
                     RMGDGVLFYHSSCAEPGIVGIARVASAAYPDPTQFDSKSPYCDPASKPESPRWVLVDV
                     QVLRKVVPTLGLRELRARPQLAEMLVLRKGNRLSITPVEPRHWKAIVGGAGRA"
     gene            complement(5240..6802)
                     /locus_tag="VGA59_12070"
     CDS             complement(5240..6802)
                     /locus_tag="VGA59_12070"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX9720458.1"
                     /translation="MNTEIVHPGRGDLTLSGFIAAACVAIVLVGAAYWLQLQQSRAAA
                     WVSHTHEVLATIARTRAALVDIQNGHRGFTISGQEEELEPYRGGRAAIAVETARLRTM
                     LADNPVQQRRLADLESGLVGRLATAAQLVEARRDGGFAAAKSIVDTGLPSEEMANLRV
                     VLKSLEAEEEQVLRERLVDHELRLRWFWAGMTAVVAMLVAALAVLYLQVRRRRAAQQA
                     LLESEHRIHRMTARDLAERERAQEALHAEMRERMRVDDELQRLNRSLEALVGERTNEL
                     RSANADLVDTKQRLRDLSSQLIAAQEQERRQIARALHDDTAQALTVIRMHLTDALRGV
                     EGTTARVPDCIEVVDSAIAQIRAISLHLRPTMLDDLGLEDALEWLLDQQAKAAGWSAA
                     LEAHDIPAELPADVQTACFRIGQEALINAARHARAGVVKLRLRVVGGQLELTVSDDGT
                     GFDLERYRTPEERKKHFGLMSMAERASLVGGSLDVDTTPGGGTRIRASFPIPAEAAIA
                     QGDSPGPAEGRD"
     gene            complement(6960..>9007)
                     /gene="clpA"
                     /locus_tag="VGA59_12075"
     CDS             complement(6960..>9007)
                     /gene="clpA"
                     /locus_tag="VGA59_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEX9720459.1"
                     /translation="SAGKKQVSTLNVLVALFSEKDTHAVFLLNEQGVTRLDIVNYISH
                     GIAKNTPAAPEAAPDQEEREGASEKAPGKTALEQFAVNLNERAKKGLIDPLIGRETEM
                     ERLMQTLCRRRKNNPLLVGEAGVGKTAIAEGLAWRIVQKEVPDILAESNVYSLDMGAL
                     LAGTKYRGDFEQRLKGVLKSLKDKPNAILFIDEIHTLIGAGAASGGTLDASNLLKPAL
                     STGALKCIGATTFTEYRGIFEKDAALSRRFQKVDVVEPSVAETIEILKGLKSRFEEHH
                     SVKYAANALTAAAELSAKYINDRHLPDKAIDVIDEAGAAQRILPVGKRKKTISRTEVE
                     EIVAKIARIPPANVSNDDRGKLQTLERDLKSVVFGQDKALEVLASAVKMARSGLGKGD
                     KPIGSFLFSGPTGVGKTEAAKQLAYIMGIELIRFDMSEYMERHAVSRLIGAPPGYVGF
                     DQGGLLTEAISKKPHAVLLLDEIEKAHPDIFNVLLQVMDHGTLTDNNGRKADFRNVII
                     IMTTNAGAESLNKAVMGFTQVREQGDEMADIKRLFTPEFRNRLDATVSFKSLDEQVIL
                     RVVDKFLLVLEQQLVEKKVEVTFTDKLRKHLAKKGFDPLMGARPMQRLIQDTIRRALA
                     DELLFGRLTEGGRLTVDVELKTDEKGVETSEVMLDIQPLPRKEGKAKPEPEEVSTDQ"
BASE COUNT         1487 a         3002 c         2907 g         1611 t
ORIGIN      
        1 agttcggcgc cgggctcgat tcgctcgatc ggaaccattc cgaagcgctc gattcgcagc
       61 gggtccgcag cacgcaccgg cggcggcgga acgactgcga caggcggcgt gcccagggct
      121 tgcccgagtt cgtagttggc gacgatggtg cggttgccgc gcctgagcat cgcgcgaacg
      181 tcggaatcgg ggccgacacg gtccgcgcgc ttgagcgtat agcgcccgac atagacgccc
      241 ggcgcggtct cgcgcagcgc aatgttttca cgcacgccgc gaatgcgcac gctggctttg
      301 acgcgcggcg tgcccaccag cctgaagcgc aggcgcgatc ccggttcgag gccggcgtcg
      361 gcctgcactg caagcgatcg aacctccggt gtggccggct gcgccaacgc ggcagacggt
      421 agtgccacca gcgaaaccgt ggctggcaag agcagcatgg cggacgtgac gtagttgcgc
      481 agatgtgttt tcatttttcc ctgacaaagt gaacggtggt tgttcaacgt tcggccctgc
      541 cgggcagaag acttccccca ggtgaagcag gtagccaaac gtaagcaatg aagctcgacg
      601 acgagcggtc gagtcacttg ccgcgtctcg tccgacgcca ccacgcggct ggccggctcc
      661 tcaggccatc acggtcctgc cgttggcgga catcccatcg gcgcacatgc cgcccttcgt
      721 gagttcgtgg tgggcctgct cgaaaccggt cctcggaacg atctcgccgg tgcggtccct
      781 gatctcgtcg aatctcgcgc ggacttgcgc cgtcgcgtcc tccgagtggc cgacgtggat
      841 gccttgcgtc atcgtgatgt gggcacattc gaagctgccg gcaccgctca tcaggatggt
      901 gcgcgtgggc gcgtcgtcgc tcacgagcgc caccaggccg gcgctcacgg cagagggatg
      961 aagccttgca agcacgtcct gctgcaagat gccctcggtc atcgccgtgg cggccgtggg
     1021 cgcgaggcaa ttgacgcgga cgttgttcct ttcaccctcg agcgccagcg tctgcatcag
     1081 cccgacgagc gccatcttgg cggcgccgta gttggcctgc ccgaagtttc cgtacaggcc
     1141 cgaggaggac gtcgtcatca cgatccggcc gtagttctgc tcgcgcatca gggaccacac
     1201 cgccttggtg cagttgaccg aacccataag gtggacgtcg atcaccagcc ggaagtcgtc
     1261 gagcgtcatc ttcgcgaagc tcttgtcgcg cagaattccc gcattgttga cgagcacgtc
     1321 gacgcggccc cacgcctgga tagcttgctc gaccatccct tccatggcac tgaagcttgt
     1381 gacgtcgccg ccattcacga tcgcttcgcc accgcagcgc cggatttcat cggcgaccgc
     1441 gcgggcgcca gagccctcgg cgtcggctcc caggtcattg accaccacgc gagcgccgtg
     1501 tttcgccagt tcgatggcat gtgaacggcc cagaccccca ccggcaccgg tgacgatggc
     1561 caccttgcct tgcaaagacc tactcataag gatttgcccc ttttcttcct caaaactcat
     1621 cagttgagga atcatacggg gacaggccga tacactgttg agccaggtag catgctgcga
     1681 tggaacgctg ctttttcggt atttctacgt attcgaaacg ttgttttcgg cccaacgaga
     1741 ggcgtcagga tcgcaggccc cggatcaacc cgttgatgca gcggatttcg gtgcggtgga
     1801 cttcggctgg gacaaaataa tctcgccgag caccgaggcg gtaatcacga cgatcgcgag
     1861 aatctgcagc agcgtcagcg actggcccag gatgatcacc gcgagcaagg ccgcaatgac
     1921 gggcttcagg aagaacaggt agcttccgcg ggtgatgtcc ggtacggccg ccaggccgcc
     1981 aatccacagg aactgggtga tggtggtatt ccagaacgca atcgtcagaa gggacagcag
     2041 tgcagtcgat tccattgccg tcaggcgttc cgggctgacc catacgccga aaaacactcc
     2101 caccaccagc cacagcccaa tggcgccgat gcccatggtg atggcggtga tgcgcagcgc
     2161 gccgtactgt tcgatgatcg ggcggaccat cactgaatag gcggccacga acgctgcgca
     2221 cagaatcgcc agcacgatgc cgaacacgtt cgcgctggat ccggccagtg cgcccaggta
     2281 gccgtcggtg atcaggagcg tgatcccgat gaccgcgcag gtgccactga cgatcttggc
     2341 cgtcgtgaac tgtttcttgt tgatcaggag attgctgatt ccgacgaaga tcggaatggt
     2401 ggtgaccacg gtcgccacct gcgctaccgt ggcgaaatcc agcgcccagt gaaagaccag
     2461 atacccgaag gtgatgccga gcaccgacag ccccagcaat ggccaaccat gggtgcgcat
     2521 gggggcaacg aggttgcggc tggacggaaa acacagcgcg agcaggacca gggcgaagcc
     2581 accgagcagg aagcgccaga ccgaaagctc cggccccttg acgcccgaca acaccgcgaa
     2641 gaactcggtc gaggcgtgac cgagcacgcc aatgaatacc gccaggtacg cgatccaggc
     2701 atgccggcgc cagagtgaca tttcagcact ccctggcgaa gtcgagcagc gccgatgcca
     2761 aggcttcggg gcgttcggcc ggcagcaggt gggcggcgtc ggccatgttc agccggcgcg
     2821 cctcgggcag ccgggcgaag agccggttga cgtcgtcttg atcgaggaac acgtggtcct
     2881 gaagcccggt gatcaccagc accggaagcg cgaggtcctc gtagggcagg tcccagtcgg
     2941 cactgccggc attggcgagc aactggtagt ggccgctgtc gggcgcaagc ggctgatacg
     3001 ggccgggttt caggaagttc gcgcgattgg ccgcgagcca gtcctgattg aacaacaagg
     3061 gagcgtacag atcccggaac aatgtcaacc cgcccacttc ggcgcaacga accagcgcgt
     3121 cgttgaccca gcgcaggcgt ggtccgtcgc ttcgcaatgt attgatcagc acgagaccgc
     3181 gcaccttgag cccgcccagg cccgccagcc aggcctgcgc ggcaaacagg ccgccgatcg
     3241 acaacccggt cagcacgatg ttctgtggcg cgagcgccgt gagcaggcga cgcgcatcct
     3301 cgacgatgcg cgttgcatcg agcctggtgc ccggtgcaaa cgggctgccc gtctggccgc
     3361 gaaaattgaa cgaaagcgtg ccatgtccgg cctgcctgag gcagctgccg atcgcgcctt
     3421 cccacatgcc ggtatcgccg gtgagcgcat tgaaaaaaac aaacgtcatc ccctccggcg
     3481 ccgccggcgg cgtgtgcagg taaaaaagcg cgttgtcgga gtcgatttca aaagtcggca
     3541 ttgcgcgtct cctgtcatgt tgtctgcctg atcggcctgt gccgacgaag cttgtagttc
     3601 gactcaagct agctgattgt gccgctgacc gcgagccata cctggaacaa acgcggcttc
     3661 aaaaacgcac catgttgcgt ttcagcaatg acaacctcgc tccgttggcg ccgtcgtagt
     3721 gggaaagtgg gacacccgtg cggcgaagtt ctaattggct tgcttgcctt ccatcaactt
     3781 caccaacgcc cacagcaccc ggttcgccgg cgtcgcaatt cccagcgcct ggccgcgccg
     3841 cacgatcagt ccattgaggt agtcgatctc gctgcgcttg ccgcgagcca ggtcctgcgc
     3901 ggtggacgaa tactggcccg gcatggtctc gacgatcttg cccacggccg catgcacgtc
     3961 gccgggcagc tgcacgcctt cggccctggc caccgccagg cattcgtcca ccacctcgcg
     4021 catcacgtcc ttcacgccct cgcccgccac ggcttcgcca tagggccgct gggcgatggc
     4081 agagaccgcg ttgtaggcgc agttgatcac cagcttggtc cagagcgcgc cgcggacgtt
     4141 gtccgagatg tcggtcggca cgccggcggc gatcagcgcc ctggccactg catcaccggc
     4201 gccggaaggc tcgatcacca gctcgccgcg gccgtggtgc ctgacgtggc cgggaccggc
     4261 catctcggtg gccacgtaca caaccgcggc cgagacctgg tgaggcggca gcacggtgcg
     4321 caaccggtcg gcgttgtcca cgccgttctg cagggtcagc accagggcct cgggcgcgag
     4381 gtgcggccgc atctgggcgc cggccgcttc ggtatcggtg gacttgacgc agaacaggac
     4441 caggttcgcg ccttgcaccg cgccgggctc ggtgctcgcg gccaggcgca cgtgctcgtc
     4501 gaaagtcctg gtctccatcc gcaggccgtc gcgctcgatg gcttccacgt gcggcggccg
     4561 cgcgatcagc accacgtcat ggccggcccg cgcgagcatg ccgccgtaat agcagcccac
     4621 ggcgcccgcc cccatgacgg cgactttgaa gggtttggtc attggcagca ttctaggaag
     4681 gtcgggaata atggctggcg gggctctcgc cctgcttgat cgaccacgaa tcctatgaac
     4741 tactggctga tgaaatccga gcccgccgaa tgctcggtgg acgatgcgct ggccgcgccc
     4801 aatgccaccg tgccctgggt gggtgtgcgc aactaccagg cccgcaattt catgcgcgac
     4861 cggatgcgaa tgggcgacgg ggtgctgttc tatcactcca gctgcgcgga gccgggcatc
     4921 gtgggcattg cccgcgtggc ctcggctgcc tatccggatc cgacgcagtt cgacagcaag
     4981 tcgccctatt gcgaccccgc atccaagcct gaatcgccgc gctgggtgct ggtcgacgtc
     5041 caggtgttgc gcaaggtggt ccccaccctc ggcctgcgcg agctgcgcgc caggccacag
     5101 ctggccgaga tgctggtgct gcgcaaggga aaccggcttt cgattacgcc cgtggagccg
     5161 cggcactgga aagcgattgt cgggggagcc gggcgcgctt gacggggcca gggcctgtga
     5221 acagggcccc tggaccagcc tagtcgcggc cctcggccgg cccggggctg tcgccctgcg
     5281 cgatcgccgc ttcggccggt atcgggaagc tcgcgcggat gcgtgtgccg cctcctggcg
     5341 tcgtgtcgac atcgaggctt ccgccaacca ggctggcgcg ttcggccatc gacatgagcc
     5401 cgaaatgttt cttgcgctct tcgggtgtgc ggtagcgctc caggtcgaac cccgttccgt
     5461 cgtccgagac ggtgagttcc agctggccgc cgacgacgcg cagccgcagc ttcaccacgc
     5521 cggcgcgggc atgacgcgcc gcattgatga gcgcctcctg gccgatgcga aagcacgcgg
     5581 tctggacatc ggccggcaat tccgcgggaa tgtcgtgggc ttccagcgcg gcactccacc
     5641 ccgccgcctt ggcctgctgg tcgagcagcc attcgagcgc gtcctccagg cccaggtcgt
     5701 ccagcatggt cgggcgcagg tgcagcgaaa tcgcgcggat ctgggcaatg gccgagtcca
     5761 ccacctcgat gcagtcgggc acgcgcgcgg ttgtgccttc gacgccgcgc agcgcgtccg
     5821 tcagatgcat gcggatcacg gtcagcgcct gcgccgtgtc gtcgtgaagc gcgcgcgcga
     5881 tctgccggcg ctcctgttcc tgcgccgcaa tgagctgcga ggaaaggtcc cgcaatctct
     5941 gctttgtgtc gaccaggtcg gcgttggcac tgcgcagctc gttggtgcgc tcccccacca
     6001 gcgcctcgag cgagcggttc agccgctgca actcgtcgtc cacgcgcatg cgctcccgca
     6061 tttcggcgtg cagcgcctcc tgcgcgcgtt cgcgctcggc caggtcgcgg gccgtcatgc
     6121 gatgaatgcg gtgttcgctt tccagcaggg cctgctgcgc ggcgcggcgg cggcgaacct
     6181 gcaggtaaag cacggcgagg gcggcgacca gcatcgccac aacagccgtc atgccggccc
     6241 agaaccaccg cagtcgcagt tcatgatcga caagccgctc gcgcagaacc tgttcctcct
     6301 cggcttcgag gctcttcagg accacgcgaa gattcgccat ttcctcactc ggcaggccgg
     6361 tgtccacgat cgacttggct gcggcgaacc cgccatcgcg ccgggcttcg accagctggg
     6421 cggcggttgc gagccgccca accagcccgc tctccagatc ggccaggcgc cgctgctgca
     6481 cgggattgtc cgcaagcatc gttcgcaagc gggccgtttc gacggcgatc gcggcgcggc
     6541 caccgcggta cggctccagt tcttcctcct ggccgctgat cgtgaagccg cggtggccat
     6601 tctggatgtc gacgagcgcc gcgcgcgttc tggcgatcgt ggcgaggacc tcgtgcgtat
     6661 ggctgaccca ggcggcggca cggctttgct gcagctgcag ccagtaggcc gcgcccacga
     6721 gcacgattgc cacacaggcg gcagcgataa agccggacaa cgtgaggtcg ccacggccgg
     6781 gatggacgat ctcggtgttc aagaggcaaa gagacagggg acaacaggct cggccagatt
     6841 ctagggtgcg tgccgtgcta tgccccggcc gcgggtcgtc gacatcggct gccgttgacg
     6901 aatgtcaaga ccaatggaaa agccgcccgg aggcggcttt tggcgccggc tgcgcgacct
     6961 tactggtcgg tcgacacttc ttcgggctcg ggcttggcct tgccctcttt cctgggcagg
     7021 ggctggatgt ccagcatcac ttcggaggtt tccacgccct tctcgtccgt cttcagctcg
     7081 acgtccaccg tcagccgacc gccctcggtc aggcggccga acagcagttc gtcggccagc
     7141 gcgcgccgga tcgtatcctg gatcagccgc tgcatcggcc gcgcgcccat cagcgggtcg
     7201 aaccccttct tggccaggtg cttgcgcagc ttgtcggtga aggtgacttc caccttcttt
     7261 tcgaccagct gctgctccag caccagcagg aatttgtcga ccacgcgcag gatgacctgc
     7321 tcgtccagcg acttgaagct cacggtcgcg tcgaggcggt tgcggaactc cggcgtgaac
     7381 agccgcttga tgtcggccat ctcgtcgcct tgctcgcgca cctgggtgaa gcccatcacc
     7441 gccttgttca gcgactcggc cccggcgttg gtcgtcatga tgatgatgac gttgcggaag
     7501 tcggccttgc gcccgttgtt gtccgtgagc gtgccatggt ccatcacctg caacagcacg
     7561 ttgaagatgt ccgggtgcgc cttttcgatc tcgtccagca gcagcacggc gtgcggcttc
     7621 ttgctgatcg cctcggtgag caggccgccc tggtcgaagc cgacgtagcc cggaggcgcg
     7681 ccgatcaacc ggctcaccgc gtgccgctcc atgtactcgg acatatcgaa gcggatcagc
     7741 tcgatgccca tgatgtaggc gagctgcttg gccgcttcgg tcttgccgac gccggtgggg
     7801 ccggagaaca ggaacgagcc gatcggcttg tcgcccttgc ccaggcccga gcgcgccatc
     7861 ttcacggcgc tcgccagcac ttccagggcc ttgtcctggc cgaacaccac gctcttcaag
     7921 tcccgctcca gcgtctgcag cttgccgcgg tcgtcgttgg agacgttggc gggcggaatg
     7981 cgcgcgatct tggccacgat ctcctcgacc tcggtcctgg agatcgtctt cttgcgcttg
     8041 cccaccggca ggatacgctg ggccgcgccg gcctcgtcga tcacgtcgat cgccttgtcc
     8101 ggcaggtgcc ggtcgttgat gtacttggcc gacagttccg cagccgccgt cagcgcgttg
     8161 gccgcgtact tgacgctgtg gtgttcctcg aagcgcgact tcaggccctt gaggatctcg
     8221 atcgtctccg cgacgctcgg ctcgaccaca tcgactttct ggaaacggcg cgacagggcc
     8281 gcgtctttct cgaaaatgcc gcggtactcg gtgaacgtgg tcgcgccgat gcacttgagg
     8341 gcgcccgtgc tcagagccgg cttgagcagg ttggatgcgt cgagcgtgcc gcccgatgcg
     8401 gcgcccgcac cgatcagggt gtggatttcg tcgatgaaca ggatcgcatt gggcttgtcc
     8461 ttgagcgact tgagcacgcc cttcaggcgc tgctcgaagt ccccgcggta cttggtgccc
     8521 gccagcaggg cgcccatatc cagcgaatac acattggatt ccgccaggat gtcgggcact
     8581 tccttctgca cgatgcgcca ggccaggccc tcggcgatcg ccgtcttgcc cacgccggcc
     8641 tcgccgacca gcagcgggtt gttcttgcgc cgccggcaga gcgtctgcat caggcgctcc
     8701 atctcggtct cgcggccgat cagcgggtcg atcaggccct tcttcgcgcg ctcgttcaga
     8761 ttcaccgcga actgctccag cgcggtcttg ccgggcgcct tctcggacgc gccctcgcgc
     8821 tcctcctggt cgggcgcggc ttcgggcgcc gccggcgtgt tcttggcgat gccatgcgaa
     8881 atgtagttga cgatgtcgag ccgcgtgaca ccctgctcgt tcagcaggaa cacggcgtgc
     8941 gtgtcttttt cgctgaacag cgcgaccagc acgttcagcg tgctgacctg cttcttgccg
     9001 gccgact
//
LOCUS       DASWIY010000337         5249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_219804, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000337 DASWIY010000000
VERSION     DASWIY010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219804"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..614
                     /locus_tag="VGA59_12080"
     CDS             117..614
                     /locus_tag="VGA59_12080"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX9720460.1"
                     /translation="MNYSLPEFLAHAIALEKEAADRYLELADMMEAHRNDAVSKLFRD
                     MVVFSTLHHDSIVERAGTMQLPRLKSWQYRWSSPPEAGGEEAFDYLLEPYNALRYARE
                     NEVRAMEYYSSVGLQTSDPEIRRMASEFAGEESEHVDALDQWLARTPRPSATWADDPH
                     RDSQP"
     gene            complement(829..1248)
                     /locus_tag="VGA59_12085"
     CDS             complement(829..1248)
                     /locus_tag="VGA59_12085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9720461.1"
                     /translation="MSKTTTMHVEIRFGDCDPAGIVFFPRYHRWMDASSLHFFMTCGV
                     PPWYVLETTKGIIGTPLLEHQARFLKSATYGERIMISTTVEEWRAKVFIQKHTITRGE
                     DLICESRETRVFCVRDPQDRTRIRSIPIPDDIRQLCE"
     gene            complement(1400..1648)
                     /locus_tag="VGA59_12090"
     CDS             complement(1400..1648)
                     /locus_tag="VGA59_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720462.1"
                     /translation="MERVTGPLFGYFVQAQSQATQQGFIATAGIFKQKPDDRPGGLWG
                     IYSVRTQTAYESELVALQAAESLANKVITHELAVFGNW"
     gene            complement(1803..3371)
                     /locus_tag="VGA59_12095"
     CDS             complement(1803..3371)
                     /locus_tag="VGA59_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX9720463.1"
                     /translation="MTEPTLARPLPERFNFAQYLLECNTDRGSKTAFIDDAGTLSFAE
                     LDERVRRMAGALTAAGIKREERVLVLMHDCNDWPVSFLGAMYAGAVPVAVNTLLTADD
                     YAYMLENSRAQAALVSGALLPALTAAMTRSEHEVHTVVVSRPVASLRPSEMDFESFLK
                     DARAAVKPAATGGDDPGFWLYSSGSTGRPKGTVHSHANPYWTVELYGKAVLALCEEDL
                     CFSAAKLFFAYGLGNALTFPLSVGATTLLMAERPTPEATFKRWTEQKPTVFFGAPTGF
                     AGMLASPRLPGREEVALRLVSSAGEALPADLGERFKAHFGVDIVDGIGSTEMLHIFLS
                     NRPDQVRYGTTGWPVPGYEVELRGDEGQAVPDGEPGDLYIHGPSSAMMYWGNRAKTRE
                     TFQGGWTKSGDKYIRNSDGTYTYAGRSDDMLKVSGIYVSPFEVEATLVQHPAVLEAAV
                     IGKEDDEGLTKTKAFVVLKPGQQASEAELKAFVKERLAPFKYPRFIEFLPDLPKTATG
                     KIQRFKLRELESKQ"
     gene            complement(3413..3877)
                     /locus_tag="VGA59_12100"
     CDS             complement(3413..3877)
                     /locus_tag="VGA59_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4863 family protein"
                     /protein_id="HEX9720464.1"
                     /translation="MSPAEFGLQIAQLTASIAGRPLDAELDAWLNEQHGPGSATFEAL
                     KSSCVAGVAEGWLCEREGGGIRYGRIFKPSADLHGFSVDVVDMKDIAGPHHTHPNGEI
                     DLVMPIEGDAHFDGRAAGWLVCPPGSAHRPTVTAGRALVLYLLPDGQIEFTR"
     gene            4010..4939
                     /locus_tag="VGA59_12105"
     CDS             4010..4939
                     /locus_tag="VGA59_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9720465.1"
                     /translation="MELEQIDGATLTGEPRPLGDKNAFLVALGERVRTLRARRGLTRK
                     SLAEAAGVSLRHLANLEYGLGNASILVLLQVAQALQCSLAELIGDVTTASPEWLLIRE
                     LLEDRDDRTLRRVRVAIGEMLGTGGDNVSRSSRIALIGLRGAGKSTLGRMLAEDLGFP
                     FVELSREIEQLAGCNISEIQALYGVNAYRRYERRAIEEVIQIYPEAVIAAPGGLVSDV
                     ATFNQLLSHCTTVWLRANAEDHMKRVAAQGDMRPMAASKEAMEDLKGILAGRTAFYSK
                     AEFSLDTSAQALEPTFMALRTLVRGALRLPVVQ"
     gene            5047..>5249
                     /locus_tag="VGA59_12110"
     CDS             5047..>5249
                     /locus_tag="VGA59_12110"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308957.1"
                     /note="cleaves the ring of
                     2,3-dihydro-2,3-dihydroxybenzoyl-CoA forming
                     6-hydroxy-3-hexenoyl-CoA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA-dihydrodiol lyase"
                     /protein_id="HEX9720466.1"
                     /translation="MTATPARVSYQTHPSQYRHWKLRFEGPVATLQADFDENAGLRPG
                     YKLKLNSYDLGVDIELNDAINRIR"
BASE COUNT          961 a         1676 c         1639 g          973 t
ORIGIN      
        1 caacttgcct ctagcggtga ctgatccgcc ggtccggatg cactccggca cgacgagctg
       61 cgcgagggcg gccgtccgaa ttgacatgcc ccatacctga ccctagagtg ggcgccatga
      121 attacagcct gcctgaattt cttgcccatg ccatcgcact cgagaaggaa gcggcggatc
      181 gttacctcga gctggcggac atgatggagg cccatcgcaa cgacgcggtg tccaaactgt
      241 tccgcgacat ggttgtattt tcgaccttgc accacgactc catcgtcgaa agagccggca
      301 cgatgcagct gcccaggctc aagtcatggc agtaccgatg gtccagcccg ccggaggccg
      361 ggggggaaga ggcgttcgac tacctgctcg agccttacaa cgccctgcgc tacgctcgcg
      421 agaacgaggt gcgggccatg gagtactaca gctcggtagg gctgcaaacg agcgaccccg
      481 agatcaggcg gatggcgtcg gaattcgcgg gtgaggaatc ggaacacgtt gacgccctgg
      541 accagtggct tgcgcgcacg cctcggccct cggccacctg ggcggacgat ccgcatcgcg
      601 acagccagcc ctagtcacgg aattgtcatg ccgggcttga cccggcatcc atgaattcat
      661 ggattgcggc tcaagggccg caatgacaag aaatgctgtc atcccgggct tgacccggga
      721 tccatgtcac cggagcagcg ccactatgga ttgcgggtca agcccgcaat gacatcgata
      781 tagacagcag tgggtcaagc ccgggatgac aattccccgg ggatcgcgct actcgcacag
      841 ctggcgaatg tcgtccggga tcggaatgga acgaatccgc gtgcgatcct gcgggtcgcg
      901 cacacagaac acgcgggttt ccctgctctc gcatatcagg tcttcgcccc tggtgatcgt
      961 gtgcttctgg atgaatacct tcgcgcgcca ttcttccacc gtggtactga tcatgatgcg
     1021 ctcgccatag gtggcgctct tgaggaagcg cgcctgatgc tcgagcagcg gcgtgccgat
     1081 gatgcccttt gtcgtctcca ggacgtacca cggcgggacg ccgcacgtca tgaagaaatg
     1141 cagcgaggac gcatccatcc atcggtgata gcggggaaaa aacacgattc cggcgggatc
     1201 gcagtcaccg aatcgaattt cgacatgcat cgtcgttgtc ttgctcatca atatccctct
     1261 tgcgcggcaa gcgttgattc tcgcagcgtc gcgtcaccgc tgtttttact catgcgtgga
     1321 tcggccccgc ggtggggcga tgtcgaccgg gcatatggct tcgtctcgtg cacgggtcga
     1381 cgagaagccg gtcgccgcgc taccagttcc cgaaaacggc taactcatgg gtgatcactt
     1441 tgttggcaag tgactcggcg gcttgcaacg cgacaagttc cgattcatac gcggtttgcg
     1501 tgcgcacgct gtagatcccc cacaggcccc caggtctgtc gtcgggcttc tgcttgaaga
     1561 taccggctgt cgcaatgaac ccctgttggg tggcttggga ttgtgcttga acaaaatagc
     1621 cgaacaacgg ccctgttacg cgttccatgc ggcgtcgccc ctttcgctcg ggtcataacg
     1681 ccaactgtgg gcccgaacat gcgcactggc atcggggact acccgccggc cggctactta
     1741 tttttgttag gagagcgctg ccgactccgc ttcgcccggg ccaggcggct cgccgcgccc
     1801 attcactgct tcgactcgag ttcgcgcagc ttgaagcgct ggatcttgcc cgtggcggtt
     1861 ttgggcaggt cgggcaggaa ctcgatgaag cgcgggtact tgaacggcgc gaggcgctcc
     1921 ttcacgaagg ccttcagctc cgcctcgctg gcctgctggc cgggcttaag cacgacgaag
     1981 gccttggtct tggtcaagcc ttcgtcgtct tccttgccga tgacggccgc ctcgagaact
     2041 gccgggtgct gcaccagagt ggcctcgacc tcgaatggcg agacgtagat gccgctcact
     2101 ttcagcatgt cgtcgctgcg acccgcatag gtgtaagtgc cgtcggagtt gcgtatgtac
     2161 ttgtcgccgc tcttggtcca gccaccctgg aaagtctctc gcgtcttggc ccggttgccc
     2221 cagtacatca tggcgctcga aggtccatgg atatagaggt cgcccggctc gccatcgggc
     2281 acggcctggc cttcgtcgcc gcgcagttca acctcgtacc ctggaaccgg ccagcccgtg
     2341 gtgccgtagc gcacctgatc gggccggttc gaaagaaaga tgtgcagcat ttccgtggag
     2401 ccgatgccat ccacgatgtc cacgccgaaa tgcgccttga aacgctcgcc cagatctgca
     2461 ggcagggcct cgcccgccga agacaccagg cgcagcgcga cctcctcgcg cccgggcagc
     2521 cgcggcgacg ccagcatgcc ggcgaagccc gtgggcgcgc cgaagaaaac cgtcggtttc
     2581 tgctcggtcc agcgcttgaa ggtcgcctcc ggcgtgggcc gctcagccat caggagcgtg
     2641 gttgcgccga cgctcaacgg gaaagtgagc gcattgccca gcccgtacgc gaagaacagc
     2701 ttcgccgccg aaaaacagag gtcctcctcg cacagcgcca gcacggcctt gccgtacagc
     2761 tccaccgtcc agtagggatt ggcatgcgaa tgcaccgtcc ccttggggcg ccctgtcgag
     2821 cccgaggagt acagccagaa gccggggtca tccccacccg tggccgccgg cttgacagcc
     2881 gcccgagcat ctttcaggaa tgattcgaag tccatctcgg acgggcgcag cgatgccacc
     2941 gggcgcgaca ccaccacggt gtgcacttca tgctcggacc gcgtcatcgc tgccgtcaag
     3001 gccggcagga gcgcgcctga tacgagcgcc gcctgggcac gcgagttctc cagcatgtag
     3061 gcgtagtcgt cggccgtcag cagcgtgttc actgcgacgg gtacggcgcc ggcgtacatg
     3121 gcgcccagga agctgaccgg ccagtcattg cagtcgtgca tcagcaccag cacgcgttcc
     3181 tcgcgtttga tgccagcggc ggtcagcgcg cccgccatgc ggcgcacacg ctcgtcgagc
     3241 tcggcaaagc tcagcgttcc ggcatcgtcg atgaaggctg tcttggagcc gcgatcggtg
     3301 ttgcattcga gaaggtactg cgcgaagttg aagcgctcgg gcagcggccg cgcaagggtc
     3361 ggttcggtca tctgtgtttc cgttcttaaa aaggcggtcg cgccgcgcgc gcttatcggg
     3421 tgaactcgat ctgcccgtct ggcagcagat aaagcacgag cgcgcggccc gcggtcacgg
     3481 tgggccgatg ggcgctgccg ggcgggcaga cgagccagcc ggccgcccgg ccgtcgaagt
     3541 gcgcatcgcc ttcgatcggc atgaccaggt cgatttcccc gttggggtgg gtgtggtggg
     3601 ggcccgcgat atccttcatg tccaccacgt cgaccgagaa tccgtgcagg tcggccgatg
     3661 gcttgaagat ccggccgtag cgaatgcctc ctccttcgcg ctcgcacagc cacccttcgg
     3721 cgacgccggc cacgcagctt gacttcagcg cttcgaaggt ggcgctgccc gggccatgct
     3781 gctcgttgag ccaggcgtcc agttcggcat ccagcggacg gcccgcgatc gaggccgtga
     3841 gctgggctat ctgaagtccg aattcggcag gggacattgc ggcagctcct ttgtgacctg
     3901 ccttgcttgc cgttggcaac aagatgcagt attgtgcgcg tgtgtacttt acgacctcat
     3961 aaaaatcatg tcaagcacta tgttgcactc cccagagacg cctggagcga tggagctgga
     4021 gcaaatcgac ggcgccacat tgaccggcga gccgcggcct ctaggcgaca agaatgcctt
     4081 tctcgtcgct ttgggcgagc gcgtcaggac cttgcgcgcc cggcgaggcc tgacccgcaa
     4141 gtcgctggcc gaagctgccg gcgtgtcctt gcgccacctc gccaacctcg aatacggact
     4201 gggcaacgcc tcgatcctgg tgctgctgca agtagcccag gccctgcagt gctccctggc
     4261 cgagctgatc ggcgacgtga ccaccgcgtc gcccgagtgg ctgctgatcc gcgaactgct
     4321 ggaagaccgc gacgaccgga cgctgcgccg ggtccgtgtg gccatcggtg aaatgctggg
     4381 caccgggggc gacaacgtct cgcgaagttc aaggattgcc ttgatcggcc tgcgcggcgc
     4441 cggcaagtca acgctaggcc gcatgctggc cgaggacctg ggcttcccct ttgtggagct
     4501 gagccgggag atcgagcaac tcgccggctg caacatctcg gagatccagg cgttgtacgg
     4561 cgtgaatgcc tatcgacggt acgaacgccg ggcgatcgag gaagtgatcc agatctatcc
     4621 ggaggcggtg atcgccgctc ccggcggcct ggtgtcggac gtcgccacct tcaaccagct
     4681 gctgtctcac tgcaccacgg tgtggttgcg cgcgaacgcg gaagaccaca tgaagcgcgt
     4741 cgccgcacaa ggtgacatgc gaccgatggc cgccagcaag gaagccatgg aagacctcaa
     4801 gggcatcctg gccggccgaa cggcttttta ttcgaaagcg gagttcagcc tggataccag
     4861 cgcgcaggct ctggaaccca ctttcatggc actgcgaacc ctggtgcgag gtgcgcttcg
     4921 actgccggtc gtccagtgaa cgcaataaag cataaaagtg cttgacatga tctatgacaa
     4981 tgcactatga tgcatgtcaa ccccgcatgg catgcagttt aatgcccgtt ctttctagga
     5041 gcacccttga ccgccactcc cgcccgcgtc agctatcaga cgcatccgtc ccagtaccgc
     5101 cattggaaac tccggttcga aggtcccgtg gccaccctgc aagcagactt cgatgaaaac
     5161 gccggcctgc gcccgggtta caagctcaag ctcaacagct acgacctggg cgtcgacatc
     5221 gagctgaacg acgcgatcaa cagaatccg
//
LOCUS       DASWIY010000338         2932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2090282, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000338 DASWIY010000000
VERSION     DASWIY010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090282"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VGA59_12115"
     CDS             complement(<1..1193)
                     /locus_tag="VGA59_12115"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9720467.1"
                     /translation="MSLAFSLCLLTVLLLAGIATPISLSIIVGAVVYLAVKGQDLGLA
                     AEQMIQGLYDSFVLLAVPLFIVAANIMNAGTISERLLKFCIAVVGRFKGGLGHVNVVA
                     SIIFSGMSGSAVADAAGIGRIIIEMMRSGNRYPPGYAAAITAAAATIGPIIPPSIPMV
                     LYALVSDSSVGYLFLGGILPGLLIGAVLMALNAYQAHRRGFALEAVVPVHQLPRTTIA
                     AFPVLLMPVILLYGIYGGVSTPTEAAAVAAAYALVLAAVVYRALSWSELYQVFLSSAR
                     SSASVGLVIGGALILNYIVASENIPAQVAKAFVGLDTSPITFMIAVNVFLLLLGCLLD
                     ASTIILVIVPLFIPACRVLGIDLVYFGVITVVNCMIGLVTPPYGILLFVINATTGIPL
                     RSIIR"
     gene            complement(1190..1687)
                     /locus_tag="VGA59_12120"
     CDS             complement(1190..1687)
                     /locus_tag="VGA59_12120"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9720468.1"
                     /translation="MLRRARNWLKAAADHVTVALLAAMFAAFILQIVSRYAFNHPLGW
                     TLEMCLTTWLWLVFWASAFVLRDGDHVKFDLAYVSVGQRTRRVFALVSAVAILAGFLA
                     AMPATIDYITFYQIKKSATLRIRLDYVFSVYGLFAGAIVIRYATRLWSIVRGGAPDEL
                     EGTGP"
     gene            complement(1697..2701)
                     /gene="dctP"
                     /locus_tag="VGA59_12125"
     CDS             complement(1697..2701)
                     /gene="dctP"
                     /locus_tag="VGA59_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9720469.1"
                     /translation="MMKPVCRRALLGFAAIAALAVLLLAGAQPAHAQKVLLRLSSPAT
                     PTDQRAIALTELFAPAVASFATFEGHWNATLFKQGTELEAIARGNLEMSITSAQELAT
                     LLPEWSIFTSGYLLRDAGHQKKVFESGIMDDMKARVESKLGVKLLTVMYLGRRQLNLR
                     MDKQIRTPADLAGVKLRMPGTEAWQFLGKALGANPVPMAFTEVYTGLQTGAIDGQDNP
                     LPTDKDSKFYEVTKQIVLTSHLVDQNYLAFSKKAWDKLSAQQQAIVQKAANDAAESGR
                     KKQLALEDSLAQFFKDQGLKVYAPDVDAFRTRVQKMYLESDFAKDWPKGMVEKINAVQ
                     "
BASE COUNT          581 a          928 c          923 g          500 t
ORIGIN      
        1 tcccggatga tgctgcgcag gggaattccc gtggtcgcgt tgatgacgaa caggagaatg
       61 ccgtagggcg gcgtcaccag gccgatcatg cagtttacga ccgtgatcac gccgaaatag
      121 accagatcga ttcccaggac gcggcacgcc ggaatgaaca ggggcacaat caccaggatg
      181 atggtgcttg catccagcag gcaccccagc agcagcaaaa agacgttcac ggcgatcatg
      241 aacgtgattg gcgaggtatc caggcccacg aaggcctttg ccacctgggc cgggatattc
      301 tcgctggcga cgatgtagtt gaggatcagc gccccgccga tcaccaggcc cacggatgcc
      361 gatgagcgcg cgctggacag gaatacctgg tagagctcgg accagctcag tgcccggtag
      421 accacggcgg ccagcaccag cgcataggcc gccgcgacag cggcagcttc cgtcggcgtg
      481 ctcaccccgc catagatccc gtagagcaga atcaccggca tcagcagcac ggggaaggcc
      541 gcaatcgtcg tgcggggaag ctggtgcacc ggcacgaccg cttccagggc gaagcccctc
      601 cggtgggcct gataagcgtt cagcgccatg agcacggcgc cgatcagcag ccccggcaga
      661 atgccgccga ggaagaggta gccgacggag gagtccgata ccagcgcgta cagcaccatc
      721 gggatcgagg gtggaatgat cggtccgatg gtggccgcgg ccgcagtaat cgcggccgcg
      781 tacccgggtg ggtagcggtt gcccgagcgc atcatctcga tgatgattct gccgatgccc
      841 gccgcatcgg ccaccgcgga accggacatg ccggagaaga tgatgctcgc cacgacgttc
      901 acgtgcccca gcccgccctt gaaccgcccc accaccgcga tgcagaactt gagcagacgc
      961 tcgctgatcg tgcccgcgtt catgatgttg gccgccacga tgaacagcgg cacagccagc
     1021 agcacgaaac tgtcgtaaag cccctggatc atctgctcgg ccgccagccc caggtcctgg
     1081 cccttgacgg ccaggtacac cacggcgcca acgatgatgg agagcgaaat gggggtggca
     1141 atgcctgcca gcagcagcac agtgagcagg cacaatgaga aagcgaggct cacgggccag
     1201 tgccttccag ctcatcgggc gcaccgccac gcacgatgct ccagagccgc gtggcatagc
     1261 ggatgacgat ggccccggcg aagagtccgt agacgctgaa gacgtagtcc aggcgaatcc
     1321 ggagcgtggc gctcttcttg atctggtaga aggtgatgta gtcgatggtg gccggcatcg
     1381 ccgcgaggaa gccggccaag atcgccactg ccgagaccag cgcgaatacc cggcgcgtgc
     1441 gctgacccac ggatacgtat gccaggtcga acttgacgtg gtctccgtct cgcaggacga
     1501 aggccgaagc ccagaagacc agccacagcc aggtcgtcag gcacatctcc aacgtccagc
     1561 cgaggggatg gttgaacgcg tagcggctga caatctgcag gatgaaggcg gcaaacatcg
     1621 ccgccagcaa cgccacggtc acgtggtcag ccgccgcctt cagccaattc cgggcacgcc
     1681 gcagcatggg gcccgcctac tgcacggcat tgatcttttc gaccatgccc ttcggccagt
     1741 ccttggcgaa atcggattcg aggtacatct tctgtacgcg ggtgcggaat gcgtccacgt
     1801 cgggggcgta taccttcagc ccctgatcct tgaagaactg cgccaggctg tcttccagag
     1861 ccagctgctt cttgcgcccg ctttccgccg catcgttggc cgctttttgc acgatggcct
     1921 gttgttgcgc tgacagcttg tcccaggcct tcttggagaa ggccaggtag ttttggtcca
     1981 caaggtggct ggtgagcacg atttgcttgg tcacttcata gaacttggag tccttgtccg
     2041 tgggcagcgg gttgtcctgc ccatcgatcg cgccggtctg caggccggtg tagacctccg
     2101 tgaacgccat cggcacgggg ttggcgccca gcgctttgcc caggaactgc caggcttccg
     2161 tccccggcat gcgcagcttc acgccggcca ggtccgccgg ggtgcggatc tgcttgtcca
     2221 tgcgcaagtt aagttggcgg cggcccaggt acatcacggt cagcagcttg acgcccagct
     2281 tgctttccac ccgggccttc atatcgtcca tgatgccgct ctcgaagacc ttcttctggt
     2341 gcccggcgtc acgcagcaga tacccggagg taaagatcga ccattccgga agcagcgtcg
     2401 ccaactcctg tgcggaggtg atcgacattt ccaggttgcc gcgtgcgatc gcctccagct
     2461 ccgtgccctg cttgaacaga gtcgcgttcc agtggccctc gaaggttgca aagctcgcca
     2521 ctgccggtgc gaacaactcc gtgagcgcga tcgcgcgctg atccgtcggc gtcgcgggcg
     2581 aagacagccg caacaggact ttttgcgcgt gggcgggttg agcgccggcc agcagcaaca
     2641 cggccagcgc tgcgatagcg gcgaagccga gcagagctcg gcggcaaacg ggcttcatca
     2701 tcgtaggtcc tccctcgtga ttggacactt cgatcggccg cgcgcaacgg atgccaaggc
     2761 gagcaaccga acgcgcacac cgttatgccg gagcgcaaac gatcgaagct ttcacaaatt
     2821 atcctatgaa acaggttttg ctctatcgta tgtgaattcc ccaggcaagc gggatcttgc
     2881 ggcgtaatgg cgagttcgcc tgccggcgct ggagagagcc catccagcgt ac
//
LOCUS       DASWIY010000339         4097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2465375, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000339 DASWIY010000000
VERSION     DASWIY010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2465375"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VGA59_12130"
     CDS             complement(<1..1054)
                     /locus_tag="VGA59_12130"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEX9720470.1"
                     /translation="MKSIAPPGPAWPHSPATHTPPRPTASPVAAGFRPATAALAVAAV
                     FVAVPNPANAQAVGAQAVHGQASLLQQGNSLVVTTRNGPGTNHSAINWQSFSVPAGST
                     TQFNQPDVASTSINRVLGGNPSAIFGTLSSNGRLVLVNPSGIAVGAGAVVDTAGFTAS
                     TLRMSEADALAGRLVFGGDGLPGAPLNVQGRILARSGDVVLIAPDVQVGADALVHAPN
                     GATMLAAGQKVELTGRGLEGIRLELQAPSDQAVNLGTLQGDAVGIFAGQLKHSGLIQA
                     TGIAAEGGKVILRGGQLADIAGRVTATQGPHGGQVHATAGKVMLRSGAVIDVSGEQGG
                     GQALVGGGWQGKDGRLA"
     gene            1355..2320
                     /locus_tag="VGA59_12135"
     CDS             1355..2320
                     /locus_tag="VGA59_12135"
                     /inference="COORDINATES: protein motif:HMM:NF022839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcNAc-transferase family protein"
                     /protein_id="HEX9720471.1"
                     /translation="MTVFVSIASYCDPVLGFTLGRALATARWPDQLHFGVVDQSPAGA
                     PQPPAADVRPARLSYVRIDPLYARGPCWARAIAMSLYDGEQWFFQIDSHMDFDPRWDE
                     RLMAQALALLPGRQGLVLSAYPNAFAFEGGQVIRRPCTDKVLAHVVKPGSGFEPDHPV
                     LGFEAHPVDQNTAVPGFHVAAGCLFAPGQFVQAFPYDPFYYFHGEEQALAARLFTHGW
                     DIFHMPGQPVYHLYNTGESGAPPRPMHWDAAHDTQRQLTWWTLEQRSRARLAALLVGE
                     PLGVYGLGRVRSMADYAAFSGIDYAARTLAPRAFRPLGTAAAQAC"
     gene            complement(2308..3093)
                     /locus_tag="VGA59_12140"
     CDS             complement(2308..3093)
                     /locus_tag="VGA59_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX9720472.1"
                     /translation="MSLQLDQRRRAMLAQMGIRVDEPYRAAEVATPSPRPSARRADGN
                     PAPAGPPRAVEAVARERPGPAALEPRPAGVELMSWDELAQAVAGCRACKLCAGRRNTV
                     FGVGDAQADWLIVGEAPGENEDLQGEPFVGPAGQLLDNMLKALGLDRRHKVYIANVLK
                     CRPPGNRNPQPEEVAQCEPFLRRQVQLLQPRIILAMGRFAVQSLLDTTEPIGKLRGRA
                     YQYLGVPVVVTYHPAYLLRNLPDKAKAWADLCLAQALVRDQQA"
     gene            complement(3176..3643)
                     /gene="rimI"
                     /locus_tag="VGA59_12145"
     CDS             complement(3176..3643)
                     /gene="rimI"
                     /locus_tag="VGA59_12145"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX9720473.1"
                     /translation="MSAVFKAVEAQFEPLTEARLDEVVAIERQAYDHPWTRGNFTDSL
                     RSGYQAQLLTGGGVALGYFVAMMGVDEVHLLNITVAPRHQRQGWGRVMLDALAMWARA
                     QGAQWLWLEVRAGNARAQRIYEQHGYRRVGERRSYYPAGHGRREDAVVMSLKL"
     gene            complement(3640..>4097)
                     /gene="tsaB"
                     /locus_tag="VGA59_12150"
     CDS             complement(3640..>4097)
                     /gene="tsaB"
                     /locus_tag="VGA59_12150"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX9720474.1"
                     /translation="GLAFGAGVPVLAVDTLLAVAEEARRQEGALQVVAVLDARMDEVY
                     SAAYACGAGGWLRQGEFSLGRPEAVAVPAGWVLAGNAFAGYGERLPVAAARIEALPTA
                     AALLRLAPALIAAGQAGPAARALPLYIRDKVAQTTEERAALRAAPPIKP"
BASE COUNT          605 a         1476 c         1359 g          657 t
ORIGIN      
        1 tcgcaagccg gccgtccttg ccctgccagc ctccgccgac cagcgcctgg ccaccgccct
       61 gctcgccgct gacgtcgatc acggcgccgc ttctgagcat gaccttgccg gcggtggcat
      121 gcacctggcc gccgtgcggc ccctgtgtcg cggtgacccg gcccgcgata tcggccagct
      181 gcccgcctct gaggatcacc ttgccgccct cggccgcgat gccggtggcc tggatcagcc
      241 cgctgtgctt caactggccg gcaaagatgc cgaccgcgtc gccttgcagc gtgccgagat
      301 tcaccgcctg gtcgctgggc gcttgcagct ccaggcggat gccttccagg ccgcgacccg
      361 tcagttcgac tttctgtccc gctgccaaca tcgtggcacc gttcggagcg tgcaccagcg
      421 catcggcacc gacctgcacg tccggcgcga tcaggaccac atcgccgctg cgcgccagga
      481 tacggccctg aacgtttagc ggcgcgccgg gcagcccgtc gccgccgaac accagccgcc
      541 cggcgagcgc atcggcttcg ctcatccgaa gcgtcgacgc cgtgaacccc gcggtgtcca
      601 ccacagcgcc cgcccccacg gcaatgcccg aggggttgac cagcaccagc cggccattgg
      661 aggacaacgt gccgaagatg gcggacgggt tgccgcccag cacgcgattg atggaagtgc
      721 tcgcgacgtc gggctggttg aactgtgtcg tgctgccggc cgggacggag aaactctgcc
      781 agttgattgc ggagtgattg gttccagggc cgttgcgggt cgtgacgacc agggaattgc
      841 cctgctgcaa gaggctggcc tgaccatgaa cggcctgcgc acccaccgcc tgggcgttgg
      901 ccgggttggg gacggccacg aaaaccgcag cgacggcaag cgccgcggtt gcgggacgga
      961 acccggccgc gaccgggctg gcggttggcc gcggcggcgt atgggttgcg ggcgaatgcg
     1021 gccaggcagg ccccggcggt gcgatggatt tcatggttga gcggctgaca gatggcctcg
     1081 tttggaaagc cgcccccttc cgacgtcttt ccatgagctc gcgacggcgc gcgtgagtcc
     1141 tatagttgcg ttcaacggta gcacagcgtt acaggcggcg caacttgcaa cagggccaat
     1201 agggcccggg atcaagacgc ttgtgcggca aatgtcacag gctcgcggcg cagcgcgctg
     1261 ccggccgagc ggcaacagcg cgaaaagacg gtcgaccttt catggttgcc ggacaatgcg
     1321 cgttgtcgcc tcccgctgcg acgccctgat tcccatgacc gtattcgtca gcatcgcctc
     1381 ctactgcgac cctgtcctcg gattcaccct ggggcgcgcg ctggccacgg cgcgctggcc
     1441 cgaccagctg catttcggcg tggtggacca aagccccgcc ggcgcgccgc aacccccggc
     1501 cgccgacgtg aggcccgcgc gcctgagcta cgtgcgcatc gatcccctgt atgcgcgcgg
     1561 tccttgctgg gcgcgcgcaa tcgccatgtc cctgtacgac ggcgagcagt ggttcttcca
     1621 gatcgattcc cacatggatt tcgacccgcg ctgggacgag cgcctgatgg cgcaggcact
     1681 ggccttgctg cccggtcgcc agggactggt gctgtccgca tacccgaatg ccttcgcctt
     1741 cgagggcggc caggtgatcc gccgcccctg cactgacaag gtgctggctc atgtggtcaa
     1801 accggggagc ggtttcgaac cggaccaccc ggtgctcggg ttcgaggccc atccggtgga
     1861 ccagaacacg gccgtgccgg gtttccacgt cgccgccggc tgcctgttcg cgccggggca
     1921 gttcgtccag gcctttccct acgacccctt ctactatttc catggcgaag agcaggcgct
     1981 ggcggcacgc ctttttaccc atggctggga tatctttcac atgccgggcc agcctgttta
     2041 ccacctgtac aacacgggcg aatccggcgc gccgccccgg ccgatgcact gggacgcggc
     2101 ccacgacacg cagcgtcaac tcacctggtg gaccctggaa cagcgttcca gagcgcgcct
     2161 ggctgcgctg ctagtcggtg agccgctcgg ggtgtatggc ctgggccggg tgcgatcgat
     2221 ggccgactac gccgcgttca gcggcatcga ctacgcggcg cggacattgg cgccgcgcgc
     2281 cttccggccc ctcggtacgg cggcggctca ggcctgctga tcgcgcacca acgcctgcgc
     2341 caggcacaaa tcggcccagg ccttggcctt gtcgggcagg tttcgcaaca ggtacgcggg
     2401 gtgataggtc accaccaccg gcacgcccag gtactggtaa gcgcggccac gcagcttgcc
     2461 gatgggctcg gtagtgtcca gcagcgactg caccgcgaaa cggcccatcg ccaggatgat
     2521 cctgggctgc agcagctgca cctgccggcg caggaacggc tcgcattgag ccacttcttc
     2581 cggttgggga ttgcggttgc cgggcggccg gcacttcagg acgttggcga tgtagacctt
     2641 gtggcggcgg tccagcccca gggccttgag catgttgtcc agcagttgcc ccgccggccc
     2701 gacgaaaggc tcgccctgca ggtcctcgtt ttcgcctggc gcctcgccga cgatcaacca
     2761 gtcggcctgg gcatcgccaa cgccgaacac ggtgttgcgc cggcccgcgc acagcttgca
     2821 ggcgcggcag ccggccacgg cttgcgcgag ttcgtcccat gacatgagtt ccaccccggc
     2881 cggccggggt tcgagtgcgg ccggcccggg gcgctcccgc gcgacagcct cgactgcgcg
     2941 cggtggcccc gccggcgccg ggttgccgtc ggcgcgccgc gcggacggcc gcggcgacgg
     3001 cgttgcgacc tcagccgccc ggtatggctc gtcgacacgg atacccattt gcgccagcat
     3061 cgcccgccgg cgctggtcca gttgcaggct catcgagccc ccacgtttgc cgcaaaggcg
     3121 gctgcactgc cccccgaggg ggcgcgagct tgcttgaagc ggttctgcgc agcgctcata
     3181 gcttcaggct catcacgacg gcatcttcgc ggcggccgtg gccggccggg taatagctcc
     3241 tgcgctcgcc cacccgccgg tagccgtgct gctcgtaaat gcgctgcgcg cgcgcattgc
     3301 ccgcgcgaac ttccagccac agccattgtg cgccttgggc ccgcgcccac atcgccaatg
     3361 catcgagcat gacccggccc cagccctggc gctggtggcg cggcgcgacc gtgatgttga
     3421 gcagatgcac ctcgtccaca cccatcattg cgacgaagta ccccagtgcg acgccgccgc
     3481 ccgtcagcag ctgggcctgg tagcccgagc gcagcgagtc ggtgaaattg ccgcgggtcc
     3541 agggatgatc gtaggcttgg cgttcgatcg ccaccacctc gtccagccgc gcttcagtca
     3601 gcggttcgaa ctgcgcttcg acggctttga atacggcgct catggcttga tgggcggcgc
     3661 ggcacgcaag gccgcccgtt cctcggtagt ttgtgccact ttatcgcgaa tatagagcgg
     3721 caaggcacga gcggcgggcc cggcctgacc ggcggcgatc aacgcggggg ccaggcgcag
     3781 cagggcggcc gccgtgggca gggcctcgat gcgcgcggca gccaccggca aacgctcccc
     3841 atagccggca aaggcgttgc ccgccagcac ccacccggcg ggcaccgcca ccgcttcggg
     3901 ccgtcccagg ctgaattcgc cctgccgcag ccagccgccc gcaccacagg catatgcggc
     3961 cgaatagacc tcgtccatgc gcgcatcgag tacggcgacc acttgcaacg cgccttcttg
     4021 ccggcgcgct tcctcggcga cggccagcag ggtatccacc gccagtaccg gcacgcccgc
     4081 gccgaacgcc agcccct
//
LOCUS       DASWIY010000340        13305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3200786, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000340 DASWIY010000000
VERSION     DASWIY010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 13305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13305
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3200786"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /gene="truA"
                     /locus_tag="VGA59_12155"
     CDS             complement(<1..527)
                     /gene="truA"
                     /locus_tag="VGA59_12155"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX9720475.1"
                     /translation="MRLALGISYNGQTYEGWQSQPSGRTVQDRLEAALSEFAAQPIST
                     VCAGRTDAGVHALMQVVHFDARVRREEFSWLRGTNRFLPPDIAVQWARPVPDAFHSRA
                     CALARRYAYVVLESPVRPSVDAGRVGWVFRPLDGGAMQEAAALLIGQHDFSSFRAAAC
                     QAKTAVKTMNRVSIAR"
     gene            complement(567..3347)
                     /locus_tag="VGA59_12160"
     CDS             complement(567..3347)
                     /locus_tag="VGA59_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEX9720476.1"
                     /translation="MGKPHWRATALAIATAVLLAVSASDASALALGRITVLSALGEPL
                     RADIEVPEATAEELASLRAAIASPEAFRAAGFDYNPAMANIQITLLRRPDGRNFLRLS
                     SPRPVNDPFIDLILEANWASGRMVRDYTMLFDPPNLRQSAPAVAAEVPPVAPAARTPA
                     APATQAARRPAAPSAPAARASAQRAPDAATAPAGNGKQVTVQRGDTAGRIAAANKPAS
                     VSLDQMLIALLRGNPEAFMGSNVNRLKAGAVLDMPTPDQVELIPAAEARQDIIAQSRD
                     FNDFRRRLAEGLPATKVGGSERQASGRVQAQVEDRKPAAAAPDKLTLSKGAVQGKVAA
                     DKIARDRAAQDDAMRVAELNKNISDLNKLGVTAPALAGASTPKPGVALPLGTTGSAKP
                     APAAAVPTVPASTAKAAAPAAVAMAPAASTPAAKADSAPAVAAVSPPASAPVAPTLAA
                     SAPAAAGPAAPAPAASEPATAAASPASAAVAAVAPAAAASGLEPVAPPLPETSLVDDL
                     LENPIVPISVGAVLALLLGFGFYRAQQRKKSTQVDSSFLESRLQPDSFFGASGGQRID
                     TNEGNATGSSMVYSPSQLDAAGDVDPVAEADVYLAYGRDLQAEEILKEAMRTTPQRVA
                     VHSKLLEIYAKRRDAKAFELVAAEAYGLTRGEGPEWDHICELGKELDASNSLYRPGGH
                     PAQIAAAGADGSRAAAFDSVATQTMPMAAAPEKPVDLDLDLDFSIGDEPAAATPGASA
                     ATQPIRPHVAEPMPSLDMDFGSATAAVDKPAVPELPASPRSDPVRLDAPNLNVSGNDL
                     SFNATQSMKAAAPTPAPVPAADAGMIEFDLGALSLDLDTPPAAASPAPSAPVAEPDAQ
                     EAVDSTAGSPLSTGGLEDDGGDPLATKLALAQEFNAIGDPDGARSLAQEVLAEASGDL
                     KQKAQRFLAEIA"
     gene            complement(3591..4739)
                     /gene="asd"
                     /locus_tag="VGA59_12165"
     CDS             complement(3591..4739)
                     /gene="asd"
                     /locus_tag="VGA59_12165"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX9720477.1"
                     /translation="MKFLNGQQPLVGLVGWRGMVGSVLMDRMQAEGDFALIEPVFFST
                     SNVGGKAPVQAKNETQLKDAFAIEALKKCDIVITAQGGDYTTEVFAKLRATGWNGHWI
                     DAASTLRMKDDAVIVLDPVNMPVIKNALARSGRNWIGGNCTVSCMLMGVGALYKAGLV
                     EWMTTMTYQAASGGGAQHMRELLTQFGTLNGEVKALLADPKSAILEIDRKVLARQKSL
                     SAEEMANFGVPLGGSLIPWIDKDLGNGVSREEWKGGAETNKILGQGEGFGTAATPVDG
                     FCVRVGAMRCHSQALTFKLKKDVPVGEIEQLIASDNAWVKVVPNTREATIKDLTPVAV
                     TGTLDIPVGRIRKLAMGPEYLGAFTIGDQLLWGAAEPLRRMLRVLLEA"
     gene            complement(4826..5914)
                     /gene="leuB"
                     /locus_tag="VGA59_12170"
     CDS             complement(4826..5914)
                     /gene="leuB"
                     /locus_tag="VGA59_12170"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX9720478.1"
                     /translation="MKIAVLPGDGIGVEIVAEAVKVLQVLDLKFEMETALVGGAAYEA
                     HGHPLPEATLELARQADAVLFGAVGDWKYDKLDRPLRPEQAVLGLRKNLGLFANFRPA
                     ICYEQLVGASSLKSELVAGLDILIIRELTGDIYFGQPRGRRTAVDGHFPGSEEAFDTM
                     RYTRPEIERIAHVAFQAARKRSKRVTSVDKANVLETFQLWKDVVTEIGTQYPDVELDH
                     MYVDNAAMQLVKAPKKFDVVVTGNLFGDILSDEAAMLTGSIGMLPSASLNTSRQGLYE
                     PSHGSAPDIAGKGVANPLATILSAAMMLRFSLDQSQAADRIESAVKDVLASGLRTADI
                     HSDGTKMVGTREMGDAVVAAITKKTTKS"
     gene            complement(6050..6694)
                     /gene="leuD"
                     /locus_tag="VGA59_12175"
     CDS             complement(6050..6694)
                     /gene="leuD"
                     /locus_tag="VGA59_12175"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX9720479.1"
                     /translation="MQKFTTHKGLVAPMDRENVDTDAIIPKQFLKSIRKTGFGQNLFD
                     AWRYLDHGEPGQDPATRKPNPDFVLNQPRYQGASILLARKNFGCGSSREHAPWALDQY
                     GFRALIAPSYADIFFNNCFKNGLLPIVLPEAQVARLFDEVNAFPGYQLTIDLERQVVV
                     KAQGEELCFDVQPFRKFCLANGFDDIGLTLRQSDKIKQFEAQRLAAKPWLAHKL"
     gene            complement(6700..8142)
                     /gene="leuC"
                     /locus_tag="VGA59_12180"
     CDS             complement(6700..8142)
                     /gene="leuC"
                     /locus_tag="VGA59_12180"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX9720480.1"
                     /translation="MANIQEARSRTLYDKIWDEHVVHTEEDGTAILYIDRHLVHEVTS
                     PQAFEGLREAGRKVWRISSIVATADHNTPTTGWERGYDGIEDPISKEQVTTLDKNIGE
                     FGAAAYFPFLSKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEV
                     EHVMATQTLLAKKARNMLIKVEGKLAPGVTAKDIVLAIIGRIGTAGGTGYTIEFGGPA
                     IRSLSMEGRMTVCNMAIEAGARAGLVAVDEKTIQYVKGRPLAPKGVEWEQAVAYWRTL
                     QSDPGAKFDTVIELDAARIIPQVTWGTSPEMVLGVDARVPDPDKEKDASKRGAIERAL
                     TYMGLEAGKALADIYVDKVFIGSCTNSRIEDMREAAAMVKKLGQKVAKNVKLAMVVPG
                     SGLVKEQAEREGLHEIFKAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQ
                     GAGGRTHLVSPAMAAAAAVHGHFVDVRQFV"
     gene            complement(8386..9696)
                     /locus_tag="VGA59_12185"
     CDS             complement(8386..9696)
                     /locus_tag="VGA59_12185"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX9720481.1"
                     /translation="MKLADNKATLSFSNGSPNVELPVYHGSVGPDVIDIRKLYAQTGM
                     FTYDPGFLSTASCQSAITYIDGDKGELLYRGYPIEQLATKCDFLETCHLLLYGELPNA
                     QAKEKFNRTVTMHTMVNEQMQFFLRGFRRDAHPMAVMTGLVGALSAFYHDSTDITNPE
                     HREIAAIRLIAKMPTLVAMAYKYATGQPYMYPQNNLSYTANFMRMMFATPCEEYSPNE
                     VLVRAMDRIFILHADHEQNASTSTVRLCGSSGTNPFAAIAAGVACLWGPAHGGANEAC
                     LNMLHDIQAQGGIEKIGDFIRQVKDKNSGVKLMGFGHRVYKNYDPRAKLMQETCKEVL
                     GELGLGNDPLFRLAMALEKIALEDEYFVSRKLYPNVDFYSGIVQRAIGVPVSLFTAIF
                     ALARTVGWIAQLNEMIGDPEYKIGRPRQLFTGATKRDVLPLAQR"
     gene            complement(9749..10015)
                     /locus_tag="VGA59_12190"
     CDS             complement(9749..10015)
                     /locus_tag="VGA59_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX9720482.1"
                     /translation="MDDELIDQRSLSKLKWRCRRGLLENDLFIERFFDRYEARLTVRQ
                     AAALNELMDLGDNDLLDLLLRRKEPEGAIKTGDVREVLEMLRSP"
     gene            complement(10027..10731)
                     /locus_tag="VGA59_12195"
     CDS             complement(10027..10731)
                     /locus_tag="VGA59_12195"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX9720483.1"
                     /translation="MQKLTFQIYRYDPDKDARPYMQSVEVELDGSERMLLDALMKIKA
                     QDPTLSFRRSCREGVCGSDAMNINGKNGLACLTNMRTVQGTIVLKPLPGLPVIRDLIV
                     DMTQFFKQYNSIKPYLINDNVPPDRERLQSPEEREELNGLYECILCASCSTACPSFWW
                     NPDKFVGPAGLLQAYRFLADSRDEATAERLDNLEDPYRLFRCHTIMNCVDVCPKGLNP
                     TMAIGKIKELMVRRAI"
     gene            complement(10799..12607)
                     /gene="sdhA"
                     /locus_tag="VGA59_12200"
     CDS             complement(10799..12607)
                     /gene="sdhA"
                     /locus_tag="VGA59_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX9720484.1"
                     /translation="MSFTSDNIARRKFDVVIVGAGGSGMRASLQLARAGLNVAVLSKV
                     FPTRSHTVAAQGGIGASLGNMSEDNWHYHFYDTVKGSDWLGDQDAIEFMCREAPKVVY
                     DLEHMGMPFDRNADGTIYQRPFGGHTANYGEKPVQRACAAADRTGHAMLHTLYQQNVK
                     AKTTFFVEWMALDLIRDAEGDVVGVTALEMETGDLHILQARTTLLATGGAGRIFAAST
                     NAFINTGDGLGMAARAGIPLEDMEFWQFHPTGVAGAGVLLTEGCRGEGAILLNSNGER
                     FMERYAPAYKDLAPRDFVSRCMGQEILEGRGCGPNKDYIHMKLDHIGADAIHKRLPSV
                     YEIGINFANVDVTRHPIPVVPTIHYQMGGIPTNIHGQVVAPKDGDDNAVVNGLYAVGE
                     CACVSVHGSNRLGTNSLLDLLVFGRAAGNHIVEFANRNAEFKPLPAAAADLALERLNR
                     LESRAGGEYAQDVANDIRNTMQAHAGVFRSQATMDEGVKKIAQLRERVNAIGLQDKSR
                     IFNTARVEALEVDNLIEAAQATIEGAAARHECRGAHLVRDYEHPADHPTAPLGRDDAQ
                     WMKHTLWYREGNRLSYKPVKIKPLTVESVPPAVRTF"
     gene            complement(12792..13157)
                     /gene="sdhD"
                     /locus_tag="VGA59_12205"
     CDS             complement(12792..13157)
                     /gene="sdhD"
                     /locus_tag="VGA59_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX9720485.1"
                     /translation="MSVNYGSKRVVVGAHYGLRDWLSQRVTAALMALFTLIVLVQVIF
                     SKGPIGYDKWAGIFAAQWMKVLTFTIIAALLWHAWVGMRDVWMDYIKPVWLRLALQVF
                     TIVWLTACAGWALQVLWRL"
     gene            complement(13209..>13305)
                     /locus_tag="VGA59_12210"
     CDS             complement(13209..>13305)
                     /locus_tag="VGA59_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX9720486.1"
                     /translation="SREFGEKSAFFTLAASLLLTLVLGAKLFNVY"
BASE COUNT         2113 a         4219 c         4464 g         2509 t
ORIGIN      
        1 cgcgctatcg atacgcggtt catggtcttt accgcagttt tcgcctggca ggcggcggcg
       61 cggaacgagc tgaagtcgtg ctgcccgatc agcagcgccg cggcttcctg catcgcgccg
      121 ccgtcgagcg ggcgaaacac ccaccctacc cggccggcgt ccacgctggg gcgcaccggc
      181 gattcgagca ccacataggc atagcggcgc gcaagggcgc aggcgcggga atggaatgca
      241 tccggaacgg gcctggccca ctggacggca atatcgggcg gcaggaaccg gttggtgccg
      301 cggagccagg agaactcctc gcgtcgcacc cgcgcgtcga aatgcaccac ctgcatgagg
      361 gcgtgcacac cggcatccgt gcggcccgcg caaacggtgg agatgggctg ggcggcgaac
      421 tcggacaagg cagcctccag gcggtcctgt accgtgcgtc cggaaggctg gctttgccag
      481 ccttcgtaag tctggccgtt gtagctgatg cccaatgcca gcctcattgg cgggctttca
      541 ggatggcttg ggaccaggga aggggatcag gcgatttccg cgaggaatcg ctgggccttc
      601 tgtttcaggt cgcccgaagc ctcggccagc acttcctgcg cgaggctgcg cgcgccgtcg
      661 gggtctccga tcgcgttgaa ctcctgggcc aaagcgagct tggtggccaa ggggtcgcca
      721 ccgtcgtctt ccaggccccc ggtgctcagc ggcgagccgg cggtgctgtc gaccgcctcc
      781 tgggcgtcgg gctcggcgac aggcgcggac ggcgccggcg aagccgccgc aggcggagtg
      841 tccaggtcca gggacaacgc acccagatcg aattcgatca ttcccgcatc agcggccggt
      901 actggtgccg gcgttggcgc cgccgccttc atggattggg tagcgttgaa gctcaggtcg
      961 ttgccggaca cattcaggtt cggcgcatcg aggcgcaccg gatcggatcg ggggctggcg
     1021 ggcaattcgg gaacagccgg cttatccacc gccgccgtgg ccgagccgaa atccatatcc
     1081 agtgacggca tgggttcagc cacgtgcggc cggatcggct gggtggcggc agaggcgccg
     1141 ggggttgcgg cggccggttc atcgccgatc gagaaatcca ggtccaggtc gaggtcgacc
     1201 ggcttttccg gtgcggcggc catcggcatc gtctgcgtgg caaccgagtc gaaagcagcg
     1261 gcccgcgaac cgtcggcacc cgcagccgcg atttgggccg gatggccacc cggcctgtaa
     1321 agcgaattgg acgcgtccag ctccttgccc agttcgcaaa tatggtccca ttccggccct
     1381 tcgccacggg tcaggccata ggcctcggcg gcgaccagct cgaaggcctt ggcatcgcgc
     1441 cgcttcgcat agatctccag cagcttgctg tggacggcca cgcgctgggg ggtggtgcgc
     1501 atggcttcct tcaggatttc ctcggcctgc aggtctctcc cataggcgag gtagacatcg
     1561 gcttcggcca ccgggtcgac atcgcctgcg gcgtcgagct ggctgggcga atacaccatg
     1621 gacgatccgg tggcattgcc ctcgttggtg tctatgcgct ggccgccgct ggcgccaaag
     1681 aacgaatcgg gctggagcct gctttccagg aaagagctgt cgacctgtgt cgacttcttg
     1741 cgctgttgcg cgcggtaaaa accgaagccc agcagaagcg caagcaccgc gcccacagaa
     1801 atgggcacga tcgggttctc aagcaggtcg tcgaccaggc tggtttccgg cagcggcggc
     1861 gcgacgggct cgagcccgga agcggccgcc gcgggcgcaa ctgcggcaac ggcagcgctt
     1921 gcggggctcg ctgccgcggt ggccggttcg ctcgcggcag gcgccggggc ggccgggccg
     1981 gccgcagcgg gtgcggatgc agccaacgtc ggcgcgacag gagcgcttgc cggcgggctg
     2041 accgcggcga cggccggagc cgagtcggcc ttggctgcgg gagtcgaggc ggccggcgcc
     2101 atcgcgaccg ccgccggggc ggccgccttg gccgtggaag ccggaactgt cggcactgcg
     2161 gcagccggcg cgggcttggc cgagccggtg gttcccagcg gcagcgcaac gcccggcttg
     2221 ggggtgctgg cgccggccag cgcaggcgcg gtcactccca gcttgttgag gtcgctgata
     2281 ttcttgttga gttcggccac gcgcatcgca tcgtcttgcg ctgcgcggtc cctggcgatc
     2341 ttatccgccg cgactttgcc ctggactgcg ccctttgaca aggtcagctt gtccggagcc
     2401 gccgcagcgg gcttcctatc ctcgacctgc gcttgcacac ggcccgaagc ctgacgttca
     2461 ctgccaccga ccttcgttgc cggcaagcct tcggccaggc ggcggcggaa gtcgttgaag
     2521 tcgcggctct gcgcgatgat gtcctggcgg gcttcggcgg cgggaatcaa ctcgacctga
     2581 tccggcgtgg gcatatcgag caccgcgccg gctttcagcc gattgacgtt gctccccatg
     2641 aaggcctccg gattgccgcg cagcagcgcg atcagcatct ggtcgagcga gacgctggcg
     2701 ggcttgttgg cggcggcaat gcggcctgcc gtgtctcctc tttgcaccgt gacctgtttg
     2761 ccgttaccgg ccggagccgt ggcggcgtca ggcgcgcgtt gcgcgctcgc gcgagccgcc
     2821 ggcgcggatg gcgcggccgg gcggcgagcg gcttgcgtag caggcgccgc cggcgtgcgc
     2881 gccgctggcg cgacaggcgg cacttcagcc gcgaccgctg gtgcgctctg ccgcaggttc
     2941 ggcgggtcga acagcatcgt gtaatcgcgt accatgcggc cagaggccca gttggcctcc
     3001 aggatcaggt cgataaacgg atcgttgacg ggacgcgggc tcgacaggcg caggaaattc
     3061 cggccgtccg ggcggcgcag cagtgtgatc tggatattcg ccatcgccgg gttgtagtcg
     3121 aacccggcag cgcggaaagc ctcgggggat gcgatggcgg cacgcaggct ggccagctct
     3181 tcggcggtcg cctcgggcac ttcgatatcg gcacgcagcg gctcgcccag ggccgagagg
     3241 accgtgatgc gacccaacgc aagggcactg gcatccgatg cggaaacagc cagcagcacg
     3301 gccgtggcga tcgccagggc ggtcgcgcgc caatgcggct ttcccattgg tttttgcgtc
     3361 cccattgctc tgtctgcagg caactttttt ctcatcatgc gaacaaacgc ctcgccgctg
     3421 aatttgctaa aaaggaccat aacatcaatg tcttaggctg acaagctgag acagctttta
     3481 acttctgggg ggttggttcg accctcgttg caattgacgc gatgttgtca agcgacaacg
     3541 gcctgtaacc actgattaca ggccgttgcg gaaggagttc agcgcgtgac tcaagcctcg
     3601 agcagaacgc gcagcatcct gcgcagcggc tcggccgcgc cccacagcaa ctgatcgccg
     3661 atggtgaagg cgccgaggta ttcgggcccc atggcgagtt tgcggatacg gcctaccggg
     3721 atgtcgaggg tgccggtgac ggccaccggc gtcaggtcct tgatcgtggc ttcgcgcgta
     3781 ttgggcacga ccttgaccca ggcgttgtcg ctggcgatga gctgttcgat ctcccccaca
     3841 gggacatcct tcttgagctt gaaggtgaga gcctggctgt ggcagcgcat cgcgcccacg
     3901 cgcacgcaga acccgtccac cggtgtcgcg gcggtgccga aaccttcccc ctggcccagg
     3961 atcttgttgg tctcggcgcc gcctttccat tcctcgcggc tgacgccgtt gcccaggtcc
     4021 ttgtcgatcc aggggatgag cgagccgccc agcggcacgc cgaagttcgc catctcctcg
     4081 gccgacaggg atttctggcg cgccaggacc ttgcggtcga tctcgagaat ggcggacttg
     4141 ggatcggcca gcagggcttt cacttcgccg ttcagcgtgc cgaactgggt gagcaactcg
     4201 cgcatgtgct gcgcgccgcc gccggaggcc gcctggtagg tcatggtcgt catccattcg
     4261 accagccccg ccttgtacag cgccccgacg cccatcagca tgcagctgac ggtgcaattg
     4321 ccgccgatcc agttgcgtcc gctcctggcc agggcgttct tgatgacggg catgttgacc
     4381 gggtccagga cgatgacggc atcgtccttc atccgcaggg tggaagccgc gtcgatccaa
     4441 tggccgttcc agccggtcgc gcgcagcttc gcgaacacct cggtggtgta gtcgccgcct
     4501 tgggccgtga tgacgatgtc gcacttcttc agggcctcga tagcgaacgc gtccttgagc
     4561 tgggtctcgt tctttgcctg caccggcgct ttgccgccga cgttggaggt ggagaagaac
     4621 accggctcga tcagggcgaa gtcgccctcg gcctgcatcc ggtccatcag caccgagccc
     4681 accatgcccc gccagccgac caggcccacc agcggttgtt gtccgttcag gaatttcatg
     4741 tcagatcgcc ttttcaaaag ttgtcaacaa accttttgtg ccccggctcg tcggtgccgg
     4801 ggaggggcgc gacgaggcgg agctgttagc tcttggtagt cttcttcgta atcgcggcca
     4861 cgacggcgtc gcccatttcg cgggtgccga ccatcttcgt gccgtcggag tggatgtccg
     4921 ccgtgcgcag gccggaggcg agcacgtcct tcacggccga ctcgatgcgg tcggcggctt
     4981 gggattggtc gagggaaaaa cgaagcatca tggcggcaga caatattgta gccaaaggat
     5041 tggcgacgcc cttgcccgcg atgtcggggg cgctgccgtg gctgggctcg tagaggccct
     5101 ggcgggaggt attcaggctg gccgagggca gcatgccgat cgagccggtg agcatggcgg
     5161 cctcgtccga gaggatgtcg ccgaacaggt tgcccgtgac gaccacgtcg aatttcttcg
     5221 gggccttcac cagctgcatc gcggcgttgt ccacgtacat gtggtccagt tccacgtcgg
     5281 ggtactgtgt gccgatctcg gtgaccacgt ctttccacag ctggaaggtc tccagcacgt
     5341 tggccttgtc cacgctcgtc acgcgcttgc tgcgcttgcg cgccgcctgg aaggcgacat
     5401 gcgcgatgcg ctcgatttcg ggccgcgtgt agcgcatggt gtcgaacgct tcctcgctgc
     5461 cgggaaagtg gccgtcgacg gcggtgcggc ggccgcgcgg ctgcccgaag tagatgtcgc
     5521 cggtcagctc gcggatgatc aggatgtcca ggccggcgac cagttccgat ttgaggctgg
     5581 aggcgcctac cagctgctca tagcagatcg ccggccggaa attcgcgaac aggcccaggt
     5641 tcttgcgcag ccccagcacg gcctgctcgg gacgcagcgg ccggtcgagc ttgtcgtatt
     5701 tccagtcgcc cacggcgccg aacaagaccg catccgcttg cctcgcgagc tcgagcgtgg
     5761 cttccggcag ggggtggcca tgcgcctcgt acgcggcgcc acccaccaat gcggtttcca
     5821 tctcgaattt caggtccagg acctggagca ccttgaccgc ctccgcgacg atctcgacgc
     5881 cgatgccgtc acccggcaga actgcgattt tcacgatctt tcccttgcat catgatgagc
     5941 cgggcgttgc accaacgcgg actcacaggt cttgaacaat atttggaagg cgacggcgag
     6001 gtaagcgctg aacaccggct cggccagctg ccgccgcggt ccgcagcgct cataacttgt
     6061 gcgcgagcca gggcttggcg gcaaggcgtt gcgcttcgaa ctgcttgatc ttgtcggact
     6121 gccgcagggt caggccgatg tcgtcgaagc cattggcgag gcagaacttg cggaacggct
     6181 ggacgtcgaa gcagagctcc tcgccctgcg ctttcacgac cacctggcgt tcaagatcga
     6241 tggtgagctg gtagccgggg aaggcattca cctcgtcgaa caggcgggcc acctgcgcct
     6301 cgggcaacac gatcggcaac aggccgttct tgaagcagtt gttgaagaag atgtcggcgt
     6361 agctgggcgc gatgagggcg cgaaaaccgt attggtccag cgcccacggt gcgtgctcgc
     6421 gcgaggagcc gcagccgaag ttcttgcgcg ccagcaggat cgaagcgccc tggtagcgcg
     6481 gctggttcag cacgaagtcc ggattgggct tgcgcgtggc gggatcctgg ccgggctcgc
     6541 catggtccag gtagcgccac gcatcgaaca ggttctggcc gaagccggtc ttgcggatcg
     6601 acttgaggaa ctgcttgggg atgatcgcgt cggtgtcgac gttctcgcga tccatcggag
     6661 cgacaagccc cttgtgtgtg gtgaattttt gcatggtcct cagacaaact ggcggacatc
     6721 gacgaaatgg ccatgcacgg ccgccgcggc ggccatggcg gggctgacca ggtgggtgcg
     6781 gccgccggca ccctggcggc cttcgaaatt gcggttgctg gtggacgcgc agcgctcgcc
     6841 cggctcgagc cggtcggcgt tcatggccag gcacatcgag cagcccggct cgcgccattc
     6901 gaaaccggcc gccttgaaga tctcgtgcag gccttcgcgt tcggcctgtt ccttgaccag
     6961 tccggacccg ggcaccacca tcgccagctt gacgttcttc gcgaccttct ggcccagctt
     7021 cttcaccatg gccgccgctt cgcgcatgtc ctcgatgcgg ctgttggtgc acgagccgat
     7081 gaagaccttg tccacgtaga tgtcggcgag cgccttgccc gcctccagcc ccatgtaggt
     7141 cagcgcgcgc tcgatggcgc cgcgcttgct cgcgtccttt tccttgtcgg ggtcgggcac
     7201 gcgggcgtcg acaccaagca ccatctcggg cgacgtgccc caggtgacct ggggaatgat
     7261 gcgggcggcg tcgagttcaa tcacggtgtc gaacttcgcg ccggggtccg actgcagcgt
     7321 gcgccagtag gcgaccgcct gctcccactc aacgcccttg ggagcgagcg gccgtccctt
     7381 gacgtactgg atggtttttt cgtcgacggc caccagcccc gcgcgggcgc cggcctcgat
     7441 cgccatgttg cacaccgtca tgcggccttc cattgagagc gagcggatcg cggggccgcc
     7501 gaactcgatc gtgtagccgg tcccgccggc cgtgccgatc ctgccgatga tcgccagcac
     7561 gatgtccttg gccgtgacgc cgggggcgag tttgccctcc accttgatca gcatgttcct
     7621 ggccttcttg gccagcaagg tctgcgtcgc catgacatgc tcgacctcgc tggtgccgat
     7681 gccgtgcgcc agtgcgccga acgcgccgtg ggtggaggtg tgcgaatcgc cgcagaccac
     7741 ggtcatgccc ggcagcgtgg cgccttgctc gggcccgatc acgtgcacga tgccctggcg
     7801 cttggacagg aacggaaaat aggcggccgc tccgaactcg ccaatgttct tgtccagcgt
     7861 ggtgacctgc tccttgctga tcgggtcctc gatgccgtcg tagccgcgct cccacccggt
     7921 ggtcggtgtg ttgtggtcgg cggtcgccac gatcgagctg atgcgccaga ccttgcggcc
     7981 ggcttcgcgc aggccttcga acgcctgcgg gctcgtcact tcgtggacca ggtggcggtc
     8041 gatgtagagg atggcggtgc cgtcctcctc ggtatggacg acatgttcgt cccagatctt
     8101 gtcgtacagg gtgcgcgagc gcgcttcctg gatgtttgcc atggtgtact tcctgtgagc
     8161 ggccatttta ttcggtggcc agtgccggcg cgcgggaagc agaaaagccc gccgaagcgg
     8221 gcctggattg cggggccggg ccggagcgtg atgcctcggt acgtgcatag cgcaggcccg
     8281 gcattgggct cgctgcactg gacagctgcg cgccccgggc gttccgcgag cccagccccg
     8341 caacgtcaaa cctgtgccat cccggctgag ccgggatcca aggctttatc tctgtgcgag
     8401 cggcagcacg tcgcgcttgg tggcgccggt gaacagctgg cgcggccggc cgatcttgta
     8461 ctccgggtcg ccgatcatct cgttgagctg ggcgatccag cccacggtcc gcgccagcgc
     8521 gaagatggcg gtgaacagcg acacggggac gccgatggcg cgctgcacga tgccggagta
     8581 gaagtccacg ttggggtaga gcttgcgcga gacgaagtat tcgtcttcga gggcgatctt
     8641 ctcgagtgcc atggccagcc tgaacagcgg atcgttaccc aggcccagct cacccagcac
     8701 ttccttgcag gtttcctgca tcagcttggc gcgcgggtcg tagttcttgt acacgcggtg
     8761 cccgaagccc atcagcttga cgccggagtt cttgtctttg acctgccgga tgaagtcgcc
     8821 gatcttctcg atgccgccct gcgcctggat gtcgtgcagc atgttgagac atgcctcgtt
     8881 ggcgccgccg tgcgccggcc cccagaggca ggccacgccg gcggcaatcg ccgcgaacgg
     8941 gttggtgccc gagctgccgc acaggcgcac ggtcgaagtc gaggcgttct gttcgtggtc
     9001 ggcgtgcagg atgaagatcc ggtccattgc gcggacgagc acttcattgg ggctgtactc
     9061 ctcgcacggc gtcgcaaaca tcatgcgcat gaagttcgcg gtgtacgaga gattgttctg
     9121 cgggtacatg tacggctggc cggtcgcgta cttgtaggcc atcgccacca gggtcggcat
     9181 cttcgcgatc agccggatcg cggcgatctc gcggtgctcg ggattggtga tgtcggtgct
     9241 gtcgtggtag aaggccgaca gggcgccgac caggccggtc atcaccgcca tcgggtgcgc
     9301 gtcccggcgg aaaccgcgca ggaagaactg catctgctcg ttgaccatgg tgtgcatggt
     9361 gacggtgcgg ttgaacttct ccttggcttg cgcgttcggc agttctccgt acagcagcag
     9421 gtggcaggtt tccaggaagt cgcatttggt cgccaactgc tcgatcgggt agccgcggta
     9481 cagcaactcg cctttgtcgc cgtcgatgta cgtgatcgcg gactggcagg aggccgtcga
     9541 aaggaagccc gggtcgtacg tgaacatgcc cgtctgcgcg tagagctttc ggatgtcgat
     9601 cacatcggga ccgacgctgc cgtggtagac gggcagctcg acgttggggc tgccgttgct
     9661 aaacgacagt gtggctttgt tgtcagcgag tttcataggg tttccttact taatcggttg
     9721 ggaacgcagc tgaagcctgt ccccaaagtc atgggcttcg cagcatctcg agcacttctc
     9781 tcacgtcgcc tgtctttatg gcgccctctg gttccttgcg ccgaagcagc agatccagca
     9841 ggtcgttgtc tccaagatcc atcaactcgt tcagggccgc tgcctgcctc acggtaaggc
     9901 gggcttcgta ccggtcgaag aagcgctcga tgaagaggtc gttttccagc aggccccggc
     9961 ggcagcgcca cttcagcttg ctcagcgatc tttggtcgat cagttcatcg tccacaaatt
    10021 catccgtcag atcgcccgcc ggaccatcaa ttccttgatt ttgccaattg ccatcgtcgg
    10081 attcagcccc ttcgggcaga catccacgca attcatgatg gtgtggcagc gaaacaagcg
    10141 gtacgggtcc tcaagattgt ccagtcgctc agccgtggct tcgtcgcggc tgtcggcgag
    10201 gaagcgataa gcctgcagca gcccggcggg tcccacgaac ttgtccggat tccaccagaa
    10261 gctcgggcaa gccgtcgaac agctggcgca caggatgcat tcgtacaggc cgttcagctc
    10321 ttctcgctct tcaggtgact gtagccgttc cctgtccggg ggcacattgt catttatcaa
    10381 ataaggtttg attgagttgt actgcttgaa gaactgcgtc atgtccacga tcaggtcgcg
    10441 gatcaccggc aatccgggca gcggcttgag cacgatggtg ccttgcaccg tgcgcatgtt
    10501 ggtcaggcat gccaggccgt tcttgccgtt gatgttcatc gcatccgagc cgcacacgcc
    10561 ttcccggcac gagcgccgga acgaaagggt cgggtcctgc gccttgatct tcatcagcgc
    10621 gtccagcagc atgcgctcgc tgccgtcgag ttcgacctcg acgctttgca tgtatggcct
    10681 ggcgtccttg tccggatcgt agcgatagat ctggaaggtg agtttttgca tgtcgaacta
    10741 ccttttcatc aaacgaaagt ggcaggcgaa attctgccgg cctcgcaaac tttctgactc
    10801 aaaacgtgcg aaccgccggc ggcacggact ccaccgtgag gggcttgatc ttgaccggct
    10861 tgtacgagag ccggttgccc tcccggtacc acagggtgtg cttcatccat tgggcgtcgt
    10921 cacgcccgag cggcgccgtc gggtggtcgg caggatgttc gtagtcgcgc accaggtggg
    10981 cgccgcggca ctcgtgccgg gcggcggctc cctcgatcgt ggcctgggcg gcctcgatca
    11041 ggttgtcgac ttcgagggcc tcgacgcggg ccgtgttgaa gatccgggat ttgtcctgca
    11101 gcccgatcgc gttcacgcgc tcgcgcaact gcgcgatctt cttcacgccc tcgtccatgg
    11161 tcgcctggct gcggaacacc ccggcatgcg cctgcatggt gttgcggatg tcgttcgcga
    11221 cgtcctgcgc gtactcgccc ccggcccggc tctccaggcg gttcagccgc tcgagcgcga
    11281 ggtcggccgc agcggcgggc agcggcttga attccgcatt gcgattcgcg aactccacaa
    11341 tgtggttgcc ggccgcgcgg ccgaacacca gcaggtccag cagcgagttg gtgcccaggc
    11401 gattggatcc gtggacgctg acgcaggcgc attcaccgac cgcgtacaag ccgttcacga
    11461 cggcgttgtc gtcgccgtct ttcggcgcta cgacctggcc gtggatgttg gtcggaatgc
    11521 cgcccatctg gtagtggatg gtcggcacca ccgggatggg gtggcgcgtg acgtcgacat
    11581 tggcgaagtt gatgccgatc tcgtagaccg agggcagccg cttgtggatg gcatcggcgc
    11641 cgatgtgatc cagtttcatg tggatgtagt ccttgttggg accgcaaccg cgtccttcga
    11701 ggatttcctg gcccatgcag cgtgagacga agtcgcgcgg ggcgagatcc ttgtacgcgg
    11761 gcgcatagcg ctccatgaag cgctcaccgt tgctgttgag caggatcgcg ccttccccgc
    11821 ggcagccttc cgtcaacagc acgcccgcgc cggcgacgcc ggtcggatgg aattgccaga
    11881 actccatgtc ttccagcggg atgccggcgc gcgccgccat ccccaggccg tcgccggtgt
    11941 tgatgaacgc gttggtggat gcggcgaaga tgcggcccgc gccgccggtg gcgagcagcg
    12001 tggtcctggc ctgcaggata tgcaggtcgc cggtttccat ctccagagcc gtcacgccga
    12061 ccacgtcgcc ttcggcgtcg cggatcaggt ccagcgccat ccactccacg aagaaagtgg
    12121 tcttggcctt gacgttttgc tggtacagcg tgtgcagcat cgcgtggccg gtccggtcgg
    12181 ccgccgcgca ggcgcgctgc accggttttt cgccgtagtt ggcggtatgg ccgccgaacg
    12241 gccgctggta gatggtgccg tcggcattgc ggtcgaaagg catgcccatg tgctcgaggt
    12301 cgtagacgac cttgggcgct tcgcggcaca tgaactcgat cgcgtcctgg tcgcccagcc
    12361 agtccgatcc cttcacggtg tcgtagaagt ggtagtgcca gttgtcctcg ctcatgttgc
    12421 ccagcgaggc gccgatgccg ccctgggcgg cgacagtgtg cgagcgcgtc ggaaagacct
    12481 tggacaggac ggccacgtta aggcccgccc gcgccagctg cagcgaagcg cgcatgccgg
    12541 agccgccggc cccgacgatg acgacgtcga acttgcgccg cgcgatgttg tcggaggtga
    12601 agctcatggg gcccccacgt ttgccgccaa ggcggctgca ctgggctcgt ggaacgtccg
    12661 tgcgcgcagc tgtacagtgg aacgagccca atgccgggcc tccactatgc acgtgccgag
    12721 gcatgacgtt gcggcccggc cccccgaggg ggcgcgatct tgcttgaagc ggttcggcgc
    12781 ggcgatcatg ctcacagtct ccagagaact tgaagggccc agccggcgca ggctgtgagc
    12841 cagacaatgg tgaagacctg cagcgcaaga cgcagccaca cgggcttgat gtagtccatc
    12901 cacacatccc gcattcccac ccacgcgtgc cacagcaggg cggcgatgat ggtaaaggtc
    12961 agcaccttca tccactgggc ggcgaaaatg ccggcccact tgtcgtagcc gatcggtccc
    13021 ttcgaaaaga tgacctgcac gagaacgatc agggtgaaca gggccatgag ggcggccgtg
    13081 acgcgctggc tgagccagtc gcgcaggcca tagtgcgcgc ctacgacgac gcgcttggaa
    13141 ccgtagttga cggacataac cactctttca gtcagttggg ggacagggct gacgatcagt
    13201 cccgcaagtt aataaacatt gaacagcttc gcgcccagta ccaaggtgag caggagactg
    13261 gcggccagcg tgaagaaagc ggacttctcg ccgaattccc tgctt
//
LOCUS       DASWIY010000341         5670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_965617, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000341 DASWIY010000000
VERSION     DASWIY010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965617"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2035
                     /locus_tag="VGA59_12215"
     CDS             <1..2035
                     /locus_tag="VGA59_12215"
                     /inference="COORDINATES: protein motif:HMM:NF023407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HEX9720487.1"
                     /translation="NLLAYLFLILGGFLPLGKRERPLLQALGRWASGLRGWRGRSGHL
                     RGDITALFHLHWQRATSSLQLQRATGALHLAALGWAAGVAASLLVQGLVVQYRVGWES
                     TFLDAGQVHAILSLLLMPVVALFPFQPFSVQEVAALQLGAGDLGRADPRWVGMYAALL
                     LVLVIVPRAVLAAFAFWRASALARRIPLDLGDRYFQRLLSLLTPARVQLCVLTHRAED
                     RAALLRVLVQEPDVARTLISSAHGDALGFVDLSGREAPAFAVARRPGAGPAWFDRLVS
                     AVFHRGGADERSGDPGLRQARDDGDVVLHVSGAAGDLEASKRLLQWLGKPVLVLANRP
                     DGMQAEQPGLVARCESEARDIPLVAGVLSFDHFARCWIQERVLLDAIGRSLAGAKLPG
                     FGRIVAAWDDRNLARFGRSMAAVAEHLLHAAQLVEEVPSGALSVKSLVSPAERQAQAQ
                     GRQAAMDAVVRRLEVSAAQMFARLRALHGIEDAAAGALQHRLEEKFVVHQGIDTPQAG
                     LAGAATGAAMGASVDLLVGGLTLGAATALGALVGGSAAFIAAAWKNRSTSSGATVVQL
                     SDDMMQAMVEAALLRYLAVIHYGRGPAGTGNELRPFWRSDVVAAVEAHKPWLAPFWTS
                     ARAQSDEAQIAVLARELETIARKVLQTLYPSQQMNSWIADRARNDSGGLSSRT"
     gene            complement(2094..2273)
                     /locus_tag="VGA59_12220"
     CDS             complement(2094..2273)
                     /locus_tag="VGA59_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720488.1"
                     /translation="MKPLDVRSGEDRRQSPSKPEPATGTSGEGAASALEQLINQEKIR
                     VLHEPKDPPRDRASA"
     gene            complement(2437..4074)
                     /gene="ipdC"
                     /locus_tag="VGA59_12225"
     CDS             complement(2437..4074)
                     /gene="ipdC"
                     /locus_tag="VGA59_12225"
                     /EC_number="4.1.1.43"
                     /EC_number="4.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate/phenylpyruvate decarboxylase"
                     /protein_id="HEX9720489.1"
                     /translation="MNLAQRLLQALARRGATEVFGIPGDFALPLFREIERSRILPLFT
                     FSHEPAVGFAADAATRMRGGLGVAAVTYGAGAFNLVNTVAGAYAERVPLVVLSGAPAA
                     HEAASGYLLHHQVKTLDSQWRVFEELTVDRARLDDARQAPALIARVLDAALRESRPVY
                     LEIPRDMPQRPCEEVPVTPEPTLDAQALAACADELLQRLRSAKRPLLMVGVEVRRYGL
                     EARVAELARRLGIPVVTSFMGLGLLTGHDVPLLGTYLGLAGCPEVAQEVEHSDALLLL
                     GVIVSDTNFAVSGQRIDLRFAIQALCGQVTMGHHVYPEIPLGRLVDALLERIPSRRAA
                     PAQPQALPARPIDQEPALDVDASVVPADVAQAVNRLMQREGAIPLACDVGDCLFTAMD
                     IAPTALIAPGYYASMGYGVPAGLGLQAATGQRPLILVGDGAFQMTGWELGNAQRYGWD
                     PVVIVFNNASWEMLRAFEPEAGFNDLGVWDFASMAAGMGGDGHLVRTRGELERALARA
                     WATRGRFQLIDVRIAPGARSPTLTRFVQAVKRLSSGG"
     gene            4134..5189
                     /locus_tag="VGA59_12230"
     CDS             4134..5189
                     /locus_tag="VGA59_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HEX9720490.1"
                     /translation="MRCRIVESLTTTTSAPSVAPELVCPAGSLPALKAAVDNGASCVY
                     LGLRDATNARNFAGLNFDEAAIANGVRYAHQRGCKVFMALNTYPQASNPGLWRDAMDK
                     AVALGVDAVILADPGLMQYAAANHPQLRLHLSVQGSATNYEAINFYREQFGVVRAVLP
                     RVLSLAQVQQVIEKTPVEIEVFGFGSLCVMVEGRCSLSSYVTGESPNTSGVCSPAKAV
                     RWQETPKGLESRLNGVLIDRYAPGENAGYPTLCKGRFNVGDEESYYAVEEPTSLNTLE
                     LLPQLFKMGVRAIKIEGRQRSPAYVAQVTRVWREAIDQCFANSHRYSPKRAWMASLDQ
                     VAEGQQHTLGAYHRPWK"
     gene            5208..>5670
                     /locus_tag="VGA59_12235"
     CDS             5208..>5670
                     /locus_tag="VGA59_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX9720491.1"
                     /translation="MKLALGPLLYYWPRNTIFRFYDAIAATPVDTVYLGETVCSRRHE
                     LRLADWLEIAGRLRQAGKEVVLSTQVLLESGSDVSSMHKIAANGEYLVEANDMGAVHR
                     LASKGAFVAGPHLNLYNLPTLEWMAKLGASRWVIPLEMKRDDLALLQPARPA"
BASE COUNT          847 a         1908 c         2004 g          911 t
ORIGIN      
        1 gaacctgctg gcctaccttt tcctgatcct gggcgggttc ctgcccctgg gcaagcgcga
       61 gcgccccctg ctgcaggcct tgggtcgctg ggcgtcgggc ttgcgcgggt ggcgcggccg
      121 ctcggggcac ctgcgcggcg acatcacggc cctgttccac ctgcattggc aacgcgcgac
      181 ctcgtccctg caattgcagc gcgcaaccgg cgcgctgcac ctggcggcac tcggatgggc
      241 cgcgggggtg gccgcgtcgc tgctggtgca gggcctggtg gtgcagtacc gcgtaggttg
      301 ggagagcacc ttcctggacg cggggcaggt gcacgccatc ctcagcctcc tgctgatgcc
      361 cgtggtggcg ctgttcccgt tccagccgtt ctcggtgcag gaggtggccg ccctgcaact
      421 cggtgcggga gacctggggc gggccgatcc ccgctgggtg ggcatgtatg cggccttgtt
      481 gctggtgctg gtgatcgtgc cccgcgcggt gctggccgct ttcgcgttct ggcgcgcctc
      541 ggcgctcgcg cgccggattc cgctcgatct cggcgaccgc tatttccagc ggctgttgtc
      601 gttgttgacg ccggcccggg tgcagctgtg cgtgttgacg catcgcgccg aagaccgggc
      661 ggctttgctg cgggtgctgg tccaggaacc ggacgtagcc cggacactga tcagctccgc
      721 tcacggggac gcgcttggtt tcgtggacct gtcgggccgc gaggctccgg ccttcgcggt
      781 cgcgcgcaga cctggcgccg ggccggcgtg gttcgaccgt ctcgtcagtg cggtgttcca
      841 tcgaggcggc gccgacgagc gatcgggcga tcctggtctg cggcaggccc gcgacgacgg
      901 cgacgtggtg ctgcacgtgt cgggcgcggc cggcgatctc gaagcgtcga agcggctgct
      961 tcaatggctg ggcaagccgg tgctggtcct cgcgaaccgg cccgatggca tgcaggcgga
     1021 gcagccgggg ctggtggcgc gatgcgaatc ggaagcgcgc gacatcccct tggtcgcggg
     1081 cgtgctgtcg ttcgaccatt tcgcccggtg ctggatccag gaacgcgtgc tgctcgatgc
     1141 catcggccgg tccctcgccg gagccaagtt gcccggcttt ggccgcatcg tcgcggcctg
     1201 ggacgaccgc aacctcgcgc gcttcggccg ctccatggcg gccgtggcgg agcatctgct
     1261 gcacgcggcg cagctggtgg aggaagtgcc cagcggcgcg ttgtccgtga aaagcctggt
     1321 gagccccgcc gagcggcagg cgcaggcgca gggcaggcag gccgcgatgg acgccgtggt
     1381 gcggcggctc gaggtttcgg cggcgcagat gttcgcgcgg ctgcgggcct tgcacggcat
     1441 cgaggacgcg gcggccggcg ccctgcaaca ccggctggaa gagaagttcg tcgtgcatca
     1501 gggcatcgac acgccgcagg cgggcctggc gggcgccgcg accggcgcgg cgatgggggc
     1561 ttcggtcgac ctgctggtgg gaggcttgac gctgggcgcg gccacggcat tgggcgcgct
     1621 ggtgggcggc agcgccgcgt tcatcgccgc ggcctggaag aaccggtcca cgtccagcgg
     1681 cgccaccgtg gtccagctga gcgacgacat gatgcaggcc atggtggagg cggcgctgct
     1741 gcgctatctt gccgtcatcc actacggccg cggtccggcc ggcaccggca acgagctgcg
     1801 gccattctgg agaagcgacg tcgtcgcggc ggtggaagcg cacaagccct ggctggcgcc
     1861 gttctggact tccgcgcggg cgcagagcga tgaggcgcag atcgccgtgc tggcccgcga
     1921 actggagacg atcgcgcgaa aggtgctcca gacgctttat ccatcgcagc aaatgaattc
     1981 ctggattgcg gatcgagccc gcaatgacag tggagggttg tcatcccgga cttgatccgg
     2041 gatccatgaa ttcctggatt gcgagcccag cccgcgatga cagctccatc gacttatgcg
     2101 gacgcgcggt ccctgggtgg atctttcggc tcgtgcagca cccggatctt ttcctggttg
     2161 atgagctgtt cgagcgcgct cgcggcgcct tcgccggacg tgcccgttgc gggctccggc
     2221 ttgctgggcg actgcctgcg atcctctccg gaacgcacat ccagcggttt catgcggact
     2281 ccttggaagc ggggtactgc aattcccgcc tggctgttca acgcggcggc cgtcccgcac
     2341 gcctaccccc gtgtttgaag ttttgtggtg cctgcgttac ggtgtgtgtg ccgcaacccg
     2401 caggaataac gaggctttgc gagtcgcgcg cggactttag cctccgctcg acagccgctt
     2461 gaccgcctgc acgaagcggg tgagggtggg tgagcgggcg ccgggcgcga tgcggacatc
     2521 gatcagctgg aagcgcccgc gcgtggccca ggctctggcc agggcgcgct cgagctcgcc
     2581 gcgggtgcgc accaggtggc cgtcgcctcc catgccggca gccatggagg cgaagtccca
     2641 gacgccgaga tcgttgaacc ccgcttcggg ctcgaacgcc ctcagcatct cccagctcgc
     2701 gttgttgaag acgatcacca ccgggtccca gccgtagcgc tgcgcgttgc ccaattccca
     2761 gccggtcatc tggaaggcgc cgtcgcccac caggatcagc gggcgctgtc cggtggcggc
     2821 ctgcaggccc agccccgcgg gcacgccata acccatgctg gcgtagtaac ccggcgcgat
     2881 cagcgcggtg ggcgcgatgt ccatcgccgt gaacaggcaa tcgccgacgt cgcaggccag
     2941 cgggatcgcg ccttcgcgct gcatcaggcg attgacggct tgggcgacgt cggcggggac
     3001 cacggacgca tccacatcga gagcaggctc ctggtcgatg gggcgcgccg gcaatgcctg
     3061 gggttgcgcc ggcgccgcac ggcgggaggg gatgcgctcc agcagggcat cgaccagccg
     3121 cccgagcggg atttccggat agacgtggtg gcccatcgtg acctggccgc acagcgcctg
     3181 gatggcgaac cgaagatcga tgcgctgccc cgacacggcg aaattggtgt ccgacacgat
     3241 cacgccgagc agcagcagcg cgtccgagtg ctcgacctcc tgggccacct cggggcagcc
     3301 ggccaggccc aggtaggtgc ccagcagcgg gacgtcatgg ccggtgagca ggcccaagcc
     3361 catgaagctg gtgaccaccg ggatccccag ccgccgcgcc agctcggcca cacgcgcttc
     3421 gaggccgtag cgccgcacct ccacgcccac catcagcaag ggccgtttcg cgctgcgcag
     3481 gcgctgcagc agctcatcgg cgcaggcggc cagcgcctgc gcgtcgagcg tgggctcggg
     3541 cgtcaccggc acctcctcgc acgggcgttg cggcatgtcc cgtggaatct cgaggtagac
     3601 cggccgcgat tcacgcaggg ccgcatcgag caccctggca atcagagccg gcgcctggcg
     3661 ggcgtcgtcc aggcgcgcgc ggtccacggt cagttcctcg aacacgcgcc attgagagtc
     3721 cagcgtcttg acctgatggt gcagcaggta accactggcc gcctcgtgcg cggccggggc
     3781 gcccgagagc acgaccagcg gcacgcgttc ggcgtaggcg ccggccactg tattgacgag
     3841 gttgaaggcg ccggcgccgt aagtcaccgc ggccacaccc agcccgccgc gcatgcgggt
     3901 cgcggcgtcg gccgcgaagc cgaccgccgg ctcgtgcgag aacgtaaaca gcggcaggat
     3961 acggctgcgc tcgatttccc ggaacaacgg cagggcgaaa tcgccgggga taccgaatac
     4021 ttcggttgca ccacggcgcg caagagcttg caaaagtcgt tgggctagat tcatcggcgc
     4081 accttaccga cgcgcgggag cgtatgtatt gaccaggatc agagggaccg gacatgcgct
     4141 gtcgtatcgt cgagagcttg actacgacta catccgcccc ttccgttgcc ccggagctgg
     4201 tctgcccggc gggcagcctg cctgcgctca aggccgccgt cgacaacggc gccagctgcg
     4261 tctacctggg cctgcgcgac gcgaccaatg cgcgcaactt cgccggcctg aatttcgacg
     4321 aagccgccat cgccaatggc gtgcggtacg cgcaccagcg cggctgcaag gtcttcatgg
     4381 cgctcaatac ctatccgcaa gcctccaatc ccgggctctg gcgcgatgcc atggacaagg
     4441 cggtggcgct cggcgtcgac gccgtgatcc tggccgaccc cgggctcatg cagtacgcgg
     4501 ccgccaacca cccgcagctg cggctgcacc tgtcggtgca gggatcggcc accaactacg
     4561 aggccatcaa cttctaccgc gaacaattcg gcgtggtgcg cgcggtgctg ccgcgcgtgc
     4621 tgtcgctggc ccaggtccag caggtgatcg agaagacacc ggtggaaatc gaggtcttcg
     4681 gtttcggcag cctgtgcgtc atggttgagg gccgctgctc gctgtcgtcc tatgtgaccg
     4741 gcgagtcgcc caacacctcc ggcgtgtgtt cgccggccaa ggccgtgcgc tggcaggaaa
     4801 cgcccaaggg actggagtcg cgcctgaacg gcgtgctgat cgaccgctat gcgcccggcg
     4861 agaacgccgg ctaccccacc ctgtgcaagg gccgcttcaa cgtcggcgat gaagagagct
     4921 attacgcggt ggaagagccc accagcctga acacgctcga actgctgccc cagctgttca
     4981 agatgggagt gcgcgccatc aagatcgagg gccgccagcg cagcccggcc tatgtggcgc
     5041 aggtcacgcg tgtgtggcgc gaggcgatcg accagtgctt cgccaactcg caccgctatt
     5101 cgcccaaacg cgcctggatg gccagcctgg accaggtggc cgagggccag cagcacacct
     5161 tgggcgcata ccaccggccc tggaagtagg gcctggaagt cacccttatg aaactcgccc
     5221 ttgggccgtt gctgtactac tggccgcgca acacgatttt ccgtttctac gacgccatcg
     5281 ccgccacgcc ggtcgatacg gtctacctcg gcgagaccgt ctgctcgcgg cgccatgaac
     5341 tgcggctggc cgattggctg gagatcgcgg ggcggctgcg ccaggccggc aaggaagtgg
     5401 tgctctccac ccaggtgctg ctggagtccg gcagcgacgt gagcagcatg cacaagatcg
     5461 ccgccaacgg cgagtacctg gtcgaggcca atgacatggg cgcggtccat cgactggcca
     5521 gcaaaggcgc cttcgtcgcg gggccgcacc tgaacctgta caacctgccg acgctggagt
     5581 ggatggccaa actgggcgcc agccgctggg tgatcccgct ggagatgaag cgcgacgacc
     5641 tcgcgctgtt gcagccggcc cgccccgcgg
//
LOCUS       DASWIY010000342         5293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1720394, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000342 DASWIY010000000
VERSION     DASWIY010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720394"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1377
                     /locus_tag="VGA59_12240"
     CDS             100..1377
                     /locus_tag="VGA59_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9720492.1"
                     /translation="MGESLAERFGLEGLGYDWYRIGNDALAALRERDYALVISDIRLP
                     DITGDALFGQLLDDHVGLPPFIFITGHGDIDTAVSLLKAGAHDYITKPFDLDALIDKI
                     QAIVTPGCGTGGFGTSPAMRRIEAMLVRVAGSAASVLITGESGVGKERVAQALHQLTG
                     AASPFIAVNCGALTETLLEAELFGYEKGAFTGAHRTKKGLFEQANGGTLFLDEIGDMP
                     LSMQIKLLRALQERRIVRVGGETPIDVDIRLVCATHQILQERVASGAFREDLYYRINV
                     VELRIPPLRERLEDILPLARALLAEVAAANGKLPPNLTPAAEHALKAYPWPGNIRELK
                     NALERASLMSNGRSISHDLLFDRPAPKLSGHQDVASSLRDYLNECERDFIVRALGSCH
                     WQIQSCADSLGISRKNLWEKMRKLGIEKETPSE"
     gene            1714..2313
                     /gene="ccmA"
                     /locus_tag="VGA59_12245"
     CDS             1714..2313
                     /gene="ccmA"
                     /locus_tag="VGA59_12245"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEX9720493.1"
                     /translation="MLSASNLTCVRGDRSLFAGIDFEVGAGEWLHVRGANGCGKTSLL
                     RLLAGLSKPAHGEIRWCGQPANDDVFRGDLLFLGHHAAVKEDLTALENLQLASSLDGD
                     ELSREEAVAALRRFGLQGREDLPVRFLSAGQKRRVLLARLVTRKAKLWVLDEPFTALD
                     TRAVQFLGTLIGEHLEQGGMAVVTSHQAIPIAGGKGLDL"
     gene            2319..2975
                     /gene="ccmB"
                     /locus_tag="VGA59_12250"
     CDS             2319..2975
                     /gene="ccmB"
                     /locus_tag="VGA59_12250"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX9720494.1"
                     /translation="MLAVMRRDLLLAMRRKTEVLTALFFFVIVTSLFPLGIGPETALL
                     RKIAPGVLWVGALLATMLGLQRMFASDHADGTLEQMVLSPTPLFFLVAGKNAAHWLVS
                     GLPLVVLAPLLGVQFDLDPESLGVLTLGLLLGTPVLSLVGAIGAALTLGVRGGGVLLS
                     LLVLPLYIPTLIFGAGAVQAHTSGLGAGGHLSLLAALLAMALFFAPWATTAALRISLE
                     "
     gene            2972..3757
                     /gene="ccmC"
                     /locus_tag="VGA59_12255"
     CDS             2972..3757
                     /gene="ccmC"
                     /locus_tag="VGA59_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEX9720495.1"
                     /translation="MSNRIVNWFRFASPYSFYPLAGRMMPWFWTLAAVFGVAGLWVGF
                     FVAPTDATQGEGYRIIFIHVPASWLSMFIYLVMAMWAGIGLALNTRLAGMMAQALAPT
                     GAIMAFLSLWTGAFWGKPMWGTWWVWDARLTSELILFFLYLGFIALQAAIDDPRRADK
                     AGAVLALVGVVNVPIIYFSVKWWNTLHQGSSVSLTRSPSMAQTMLWGMLLMALAFWMY
                     SIAVGLKRLRTIILEREAHTDWVRNLAEVKAPAKLTKVTEVTA"
     gene            3754..3942
                     /gene="ccmD"
                     /locus_tag="VGA59_12260"
     CDS             3754..3942
                     /gene="ccmD"
                     /locus_tag="VGA59_12260"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX9720496.1"
                     /translation="MNWNSASEFFAMGGYGLYVWGSFGVCAVALALEPFLLSRRRQAI
                     LQSLRRHALAEQMDQEIA"
     gene            3939..4379
                     /gene="ccmE"
                     /locus_tag="VGA59_12265"
     CDS             3939..4379
                     /gene="ccmE"
                     /locus_tag="VGA59_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX9720497.1"
                     /translation="MKNWKKRGAVVAGGVAAVSVAATLVLNALNSNIALYVTPTEVAA
                     GKSPKGQAFRIGGMVKEGSLKRDNLTVHFVVTDTAKDIPVSYTGILPDLFKEGKGAVV
                     QGKLGTDGHFTASEVLAKHDENYMPPEAQHSLDQAKKAEGTVKP"
     gene            4376..>5293
                     /gene="ccsA"
                     /locus_tag="VGA59_12270"
     CDS             4376..>5293
                     /gene="ccsA"
                     /locus_tag="VGA59_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX9720498.1"
                     /translation="MIPEIGHFALILSLCVAVVLGTLPLVGAQQGRREWLVLARPAAQ
                     VMFLLVTVSIAALAWSFYVNDFSVAYVAGHSNSMLPTPYRLAAVWGGHEGSLLLWLFM
                     LAGWTVAVAQRSRTLDEAMVARVLGVLGLVMTGLLLFVLLTSNPFDRLIPAAAEGRDL
                     NPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIAALLSGRLDAAWARWSRPWTTTAWIFL
                     TLGIGLGSWWAYYELGWGGWWFWDPVENASFMPWLAGTALLHSALVMEKRDALKVWTI
                     LLAIITFSLSLMGTFLVRSGVLTSVHTFAV"
BASE COUNT          845 a         1691 c         1734 g         1023 t
ORIGIN      
        1 aatccaatct cgggctgacc cgctttaccg taatcattcc tttggaccct gctcatgacg
       61 caagcgccca ggatctgcct gattgaagac gatgagatca tgggcgaatc gctggccgaa
      121 cgattcggcc tcgagggcct cgggtatgac tggtaccgca ttggcaatga cgctctggcc
      181 gcgctgcgcg aacgcgatta cgccctcgtc atcagcgaca tccgcctgcc ggacatcacc
      241 ggcgacgccc tgttcggcca gctgctggac gatcacgtgg gcttgccgcc tttcatcttc
      301 atcacaggtc atggcgacat cgacaccgcc gtgagcctgc tcaaggcagg cgcgcacgac
      361 tacatcacca aaccgttcga tctggacgcg ctgatcgaca agatccaggc catcgtcacg
      421 cccggctgtg gcaccggtgg gttcgggacg tcacccgcca tgcggcggat cgaagccatg
      481 ctggtgcgag tcgccggcag cgccgcgtcg gtgctgatca ccggcgagtc aggcgtcggc
      541 aaggagcggg tggcgcaggc gttgcaccaa ttgacgggcg cagccagtcc cttcatcgcc
      601 gtgaattgcg gggcgctgac cgagaccctc ctcgaagccg agctcttcgg ctatgaaaaa
      661 ggtgcgttca cgggcgccca ccggaccaag aaaggtctgt tcgagcaagc caatggcggc
      721 acgcttttcc tcgacgagat cggcgacatg ccgctgtcca tgcagatcaa gctgctgcgc
      781 gcgttgcagg agcgccgcat cgtgcgggtg ggtggcgaaa ctccgatcga cgtcgacatc
      841 cgcctggtct gcgccacgca tcagatcctg caggagcggg tcgcgagcgg agctttccgc
      901 gaagacctgt actaccgtat caacgtggtt gaactgcgca ttccaccgct gcgcgagcgg
      961 ctcgaggaca tcctgccgct ggcgcgcgcg ctccttgccg aagtcgccgc tgccaatggc
     1021 aagctgcccc ccaacctgac accggccgcc gaacacgcct tgaaggctta cccgtggccc
     1081 ggcaacatcc gcgagttgaa aaacgccctc gagcgcgcca gcctgatgag caacggacgg
     1141 tcgatttccc atgacctcct gtttgaccgc ccggcgccaa agctgagcgg ccatcaggat
     1201 gtggccagca gtctgcgcga ttacctgaat gaatgcgaac gcgactttat cgtgcgcgcg
     1261 ctcggttcct gccattggca gatccagagc tgcgccgact ccctgggcat cagccggaag
     1321 aatctttggg aaaagatgcg caagctcgga atagaaaagg aaaccccttc agaataggtg
     1381 gtatcgaaag cacctcgctc ctggaacata ctcccgccag tacctaaata ctttgcgcac
     1441 tgtcaaagtt ccaagggttt tcccgcgaca ataggcgagt tcaggcgtta gcgaggattt
     1501 gctgccgcgc acgggccaga ggacaagaaa aacttagccg agatagcgac cggtggtgag
     1561 ctacaccgcg tgccgggttt taggggcttt ggcctgcaaa gcaggccgtg ctgaggcaca
     1621 cttggggcac ttgtccagcc cccgttgatc tacgtcaacg gggggaatct cgccgcgttg
     1681 gcagcggccc atagcctgcg gacaatccat ccaatgttga gcgcctccaa tttaacctgc
     1741 gtgcgcgggg accgttccct gttcgccgga atcgacttcg aggtcggcgc cggcgagtgg
     1801 ctgcacgtgc gtggcgccaa cggttgcggc aagaccagcc tgctcaggct gctggccggg
     1861 ctttccaagc ccgcgcacgg cgagatccgc tggtgcggcc agcccgccaa cgacgatgtg
     1921 ttccggggcg acctgctgtt cctgggtcat cacgccgccg tgaaggaaga cctgacggcg
     1981 ctggagaacc tgcagctcgc ctcgtcgctg gacggcgatg aactttcgcg cgaagaggcg
     2041 gtggccgcac tgcggcgctt cggcctgcaa gggcgcgagg acctgcccgt gcgttttctc
     2101 tcggccggcc agaagcgccg cgtcctgctg gcgcgcctgg tcacccgcaa ggcgaagctg
     2161 tgggtgctgg acgagccttt caccgcgctc gacactcgcg ccgtccagtt ccttggaacg
     2221 ctcatcggcg agcacctcga gcagggtggc atggcagtag tcacgagcca tcaggcgatc
     2281 cccatcgccg gtggcaaggg actcgacctg tgaaggcgat gctggccgtg atgcgccgtg
     2341 acctgctcct cgcgatgcgc cgcaagacgg aggtgctgac ggcgctgttc ttcttcgtca
     2401 tcgtgaccag cctgtttccc ctcgggatcg ggcccgagac ggcgctgctg cgcaagatcg
     2461 cgcccggcgt gctgtgggtc ggcgcgctgc tggccaccat gctgggcctg cagcgcatgt
     2521 tcgcgagcga tcacgccgac ggcacgctcg agcagatggt gctgtcgcca acgccgctgt
     2581 tcttcctggt ggccggcaag aacgccgcgc actggctggt ctccggcttg ccgctggtcg
     2641 tgctggcgcc cctgctgggg gttcagttcg acctggaccc cgagtcgctc ggtgtgctga
     2701 ccctggggct gctgctggga acgccggtgc tgtcgctggt aggtgccatc ggggctgcgc
     2761 tgacgctggg cgtgcgcggc ggcggagtgc tcctgagctt gctggtgttg ccgctttaca
     2821 tccccacgct catcttcggc gcgggtgccg tgcaggccca tacgtcgggc ctgggcgccg
     2881 gcggccacct gtcgctgttg gccgcgctgc tggcgatggc cttgtttttc gcgccgtggg
     2941 cgaccacggc cgccttgagg atttcactgg aatgagcaac cgcatcgtca actggtttcg
     3001 attcgccagt ccctacagct tctacccgct cgcgggcagg atgatgccct ggttctggac
     3061 gctggcggcc gtgttcggcg tcgccggctt gtgggtcggc ttcttcgtcg cgcccacgga
     3121 cgccacgcag ggcgagggct accggatcat cttcatccat gtgccggcgt cctggctgtc
     3181 gatgttcatc tatctcgtga tggcgatgtg ggccgggatc ggtctggcgc tcaacacgcg
     3241 cctggccggt atgatggcgc aggcactggc gcccacgggc gccatcatgg cgttcctgtc
     3301 gctgtggacg ggcgccttct ggggcaagcc gatgtggggc acctggtggg tgtgggacgc
     3361 gcgcctgacc tcggaactaa tcctgttctt cctgtatctt ggatttatcg cgctgcaggc
     3421 ggccatcgac gatccgaggc gcgccgacaa ggccggcgcg gtcctggcgc tggtgggtgt
     3481 ggtgaacgta cccatcatct atttctcggt gaagtggtgg aacacgctgc atcagggctc
     3541 ttcggtctcc ctgaccaggt cgccctcgat ggcgcaaacc atgttgtggg gcatgctgct
     3601 gatggcgctc gccttctgga tgtactcgat cgcggtgggg ctcaagcgcc ttcgcaccat
     3661 catcctggag cgggaagccc acaccgattg ggtgcggaac ctggcggagg tgaaggcccc
     3721 cgcgaagctg acgaaggtga cggaggtgac ggcatgaact ggaattcggc atcggaattt
     3781 ttcgcgatgg gcggttatgg gctttatgtc tggggcagct tcggcgtgtg tgccgtggcc
     3841 ctggcgctcg agccatttct tttgagcagg cgccggcagg cgatcctgca aagcctgcgc
     3901 cgccatgcgc tggccgaaca gatggatcag gagatcgcat gaaaaactgg aaaaaacgcg
     3961 gtgcagttgt cgccggcggc gtggcagccg tcagtgtcgc cgcgactttg gtgctcaacg
     4021 cactcaacag caatattgcg ctgtacgtga cgcctaccga ggtcgcggcc ggcaagtcgc
     4081 ccaagggcca ggcgttccgc atcggcggca tggtcaagga aggctcgctc aagcgcgaca
     4141 acctgacagt gcatttcgtg gtgaccgaca cggccaagga tattcccgtg tcgtacaccg
     4201 gcatcctgcc cgacctgttc aaggaaggca aaggcgccgt ggtgcagggc aagctgggaa
     4261 cggacggcca cttcaccgca tcggaggtgc tggccaagca cgacgaaaac tacatgccgc
     4321 cggaagcgca gcactcgttg gaccaggcca agaaagctga agggactgtc aagccatgat
     4381 tcctgaaatt gggcatttcg ccctgatcct ctcgctgtgc gtggccgtcg tgctgggcac
     4441 cctgccgctc gtgggcgcgc agcagggccg ccgcgaatgg ctggtcctgg cgcgccccgc
     4501 ggcgcaagtg atgttcctgc tggtcacggt ttcaatagcc gccctggcct ggagcttcta
     4561 cgtcaacgat ttctcggtcg cctatgtggc cggccactcc aactcgatgc tacccacgcc
     4621 gtaccggctt gccgcggtgt ggggcggcca cgagggatcg ctgctgctct ggctcttcat
     4681 gctcgccggc tggaccgtcg cggtcgcgca gcgctcccgc acgctcgacg aagccatggt
     4741 tgcgcgcgtg ctcggtgtgc tgggactggt gatgaccgga ctgttgctgt tcgtgctgct
     4801 cacctcgaat ccgttcgacc ggctgattcc ggcggccgcg gaaggccgcg acctcaaccc
     4861 gttgctgcaa gaccccggcc tcatcttcca tccgcccctg ctgtacatgg gttacgtggg
     4921 cttttccgtg gcgttcgctt tcgcgatcgc ggcgctgctc tccggccggc tcgacgcggc
     4981 ctgggcccgc tggtcgcggc cctggacgac gacggcctgg attttcctga ccctcgggat
     5041 cggcctgggc tcctggtggg cgtattacga gctgggctgg ggaggctggt ggttctggga
     5101 cccggtcgag aacgcctcct tcatgccctg gctcgccggc acggcgctgc tccattcggc
     5161 gcttgtcatg gagaagcgcg acgcgctcaa ggtgtggacc atcctcctcg ccatcatcac
     5221 cttctccttg agcctgatgg ggaccttcct cgtccgctcg ggcgtgctca cgagcgtgca
     5281 cacctttgcc gtc
//
LOCUS       DASWIY010000343         4183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1329850, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000343 DASWIY010000000
VERSION     DASWIY010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329850"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="VGA59_12275"
     CDS             complement(<1..1270)
                     /locus_tag="VGA59_12275"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HEX9720499.1"
                     /translation="MPDLHFPQPDSAFDALLATAVRRLLHGEALEPFCDWFAHDMASL
                     MMARLPVAPEDEESSRRYQRTVARTLWAALPVPSNRWRPRPLPKVERNDPCHCGSGRK
                     FKQCCAEFAGVAPSMEPDGLYAMALAQAEPAMLTPDQVRLVPGEALGMAAMDWNEQGQ
                     PERTAAVLAPLFQQCNDLDGRHELAFDALMDAMQEQGQETQRRALAQHVSQSRDKTLA
                     TAARCRQASMLADQGEFDAAWAMFQSAQRLSPGEVQLLHLELTMLLAQGRHDEAKLRA
                     PVLAAKARKAGHDDLAAILLQLAEGGFPDAYEDAGVQDVDDPEDLAWIALCEQTPGEF
                     APEDCRSLYRFSELPPDEAGQPPVLSVKPVKTLADLQRRWRRRFPVGKPMLTQLQGDA
                     DALLANLPAAAQFLQKNPQAWLSMEVLDDLL"
     gene            complement(1337..2104)
                     /gene="ugpQ"
                     /locus_tag="VGA59_12280"
     CDS             complement(1337..2104)
                     /gene="ugpQ"
                     /locus_tag="VGA59_12280"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX9720500.1"
                     /translation="MTSEQTWPYPRWIAHRGAGKLAPENTLAAFRLGASHGYRMFECD
                     AKLSADGVVFLMHDDTLERTTNAVQALGPGASNVAGNHPWSALSRLDAGGWHSRSYAG
                     EPLPTLDNIARFCRANGHFLNIEIKPTPGLERRTGEVVAREAARLWAGAAVPPLLTSF
                     QPEALLAAQAGAAPLPRGLLLDTLWTGWLETALSLGCVAIVCNHALWDQSSLTQARSA
                     GFRCLSYTVNDEWAAQRLLDLGTDGIITDRVDLFPPG"
     gene            complement(2101..3108)
                     /gene="ugpC"
                     /locus_tag="VGA59_12285"
     CDS             complement(2101..3108)
                     /gene="ugpC"
                     /locus_tag="VGA59_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX9720501.1"
                     /translation="MASITLKNIVKRYGSGKHELQVIHGVDAMVEDREFVVIVGPSGC
                     GKSTLLRMVAGLEEITEGEISIGNRVVNNLEPSDRDIAMVFQNYALYPHMSVFDNMAY
                     GLKIKKVPPQEIKTRVDKAAGILELGQLLERKPRQLSGGQRQRVAMGRAIVRQPQVFL
                     FDEPLSNLDAKLRAQTRIEIQKLHRELGITSLFVTHDQVEAMTLAQRMLVMNAGRMEQ
                     FGTPEEVYHQPASTFVASFIGSPPMNLLTGAPGSRAGTIMGVRPEHLDVTPSGWPLVV
                     ETTELLGAERLIYGRLGAEQVIVRAEEHTAIPQPGSTVHVTPRPDRVHWFDGASHKRI
                     P"
     gene            complement(3146..3997)
                     /gene="ugpE"
                     /locus_tag="VGA59_12290"
     CDS             complement(3146..3997)
                     /gene="ugpE"
                     /locus_tag="VGA59_12290"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HEX9720502.1"
                     /translation="MVERRPLATAISHFVLVLGVIVIAFPLYVTFIASTHTAQDIVQV
                     PMPLLPGPHLVDNYIAAIKGSTSAAASAAPVGRMMTVSLITALVIAIGKIAISLLSAF
                     AIVYFRFPGRMFFFWAIFVTLMLPVEVRIGPTYQVVSDLGMLNSYAGLTVPLIASATA
                     TFLFRQFFLTVPDELVEAARVDGAGPLRFFKDVLVPLSTTSIAALFVIQFIYGWNQYL
                     WPLLVTTDESMYPVVMGIKRMISGGDAANEWNIVMATAILAMIPPAVVVLAMQKWFVK
                     GLVDTEK"
     gene            complement(3997..>4183)
                     /locus_tag="VGA59_12295"
     CDS             complement(3997..>4183)
                     /locus_tag="VGA59_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866909.1"
                     /note="with UgpEC is involved in the uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter permease"
                     /protein_id="HEX9720503.1"
                     /translation="VDAATEGGPGKDTAILVYKVYYDGFKALDLGGSAAQSVVLMVIV
                     IALTVVQFRYVERKVNY"
BASE COUNT          736 a         1325 c         1401 g          721 t
ORIGIN      
        1 gcagcagatc atccagcact tccatgctca gccaggcttg tgggttcttc tgcaggaatt
       61 gcgccgccgc gggcaggttg gccagcagag cgtccgcgtc gccctgcagc tgggtgagca
      121 tgggcttgcc caccggaaaa cgccgccgcc agcgccgttg caggtcggcg agcgtcttca
      181 cgggcttgac cgacaggacg ggtggctgcc ctgcctcatc aggcggcagc tcactgaagc
      241 ggtacaggga gcggcagtct tccggcgcaa attcgccggg cgtctgctcg cacaaggcga
      301 tccatgcgag gtcttccgga tcgtccacgt cttggacgcc tgcgtcttca tacgcatcag
      361 ggaagccgcc ctcggccagt tgcagcaata ttgcggccag atcatcgtgg ccagccttgc
      421 gtgccttggc ggccagcacc ggggcgcgaa gctttgcttc atcatggcgg ccctgggcca
      481 gcagcatcgt cagttccaga tgcaagagtt ggacctcacc ggggctcagc cgctgtgcgg
      541 actggaacat ggcccaggcc gcgtcgaact caccctggtc ggccaacatc gaggcctggc
      601 ggcaacgcgc ggcggtggcc agcgtcttgt cccggctttg gctgacgtgc tgcgccagcg
      661 cccggcgttg cgtttcctgg ccctgttcct gcatggcatc catcagggca tcgaaggcca
      721 attcatggcg gccatccagg tcgttgcact gctgaaacag gggcgccagc acagcggcag
      781 tgcgctcggg ctggccctgc tcgttccagt ccatcgcggc catgcccagc gcctcacccg
      841 gcaccagacg aacctggtcg ggggtgagca tggccggctc ggcctgtgcc aacgccatcg
      901 catagaggcc gtcgggctcc atcgaaggcg ccacgcccgc aaactcggcg cagcactgct
      961 tgaacttgcg gcccgatccg cagtggcagg gatcgttgcg ctccaccttg ggcaaggggc
     1021 ggggccgcca ccggttggat ggcacgggca gggctgccca cagcgtgcgc gccacggtgc
     1081 gctggtagcg gcgggaactc tcttcgtctt ccggcgcgac cgggagccgc gccatcatca
     1141 gactggccat gtcgtgggca aaccagtcgc aaaacggctc caacgcctcg ccatgcaaca
     1201 ggcgccggac ggcggtggcc agcagcgcgt caaaggcgct atcgggttgg gggaagtgca
     1261 ggtcgggcat ggtcggcggg cgtgaacgaa atgctgcggc gatggtaagc catgccgcac
     1321 gatgggattg ttcagctcaa cccggcggga acaggtcgac ccggtcggtg atgatgccgt
     1381 cggttcccaa gtccagcagg cgctgagcgg cccattcgtc attgaccgta tagctcaggc
     1441 agcggaagcc ggcgcttctc gcctgggtca ggctgctttg gtcccacagg gcgtgattgc
     1501 agacgatggc cacgcagccg agcgacaggg cggtctccag ccagccggtc cacaaagtat
     1561 ccagcaacag tccgcgtggc agaggcgctg ccccggcctg cgcagccagc agcgcctccg
     1621 gctggaacga tgtgagcagg ggtggcaccg cggcgccggc ccagagccgc gccgcctcgc
     1681 gagcgacgac ttcgccggtg cgccgctcca gccccggggt gggcttgatc tcgatgttca
     1741 ggaaatggcc gttggcccgg cagaaccggg cgatgttgtc cagcgtcggg aggggttcgc
     1801 cggcatacga cctcgaatgc cagccaccgg cgtcgagccg cgacagggcg ctccagggat
     1861 gattcccggc aacgttactg gccccggggc cgagcgcttg cactgcgttg gtcgtgcgtt
     1921 ccagcgtgtc atcgtgcatc aggaaaacca cgccgtcggc cgagagcttc gcgtcgcatt
     1981 cgaacatgcg atagccatgg ctcgcgccca ggcggaacgc ggccagcgtg ttctcgggcg
     2041 caagcttgcc cgcaccgcgg tgggcgatcc agcgggggta cggccaggtc tgctcgctag
     2101 tcatggaatg cgcttgtggc tggccccgtc gaaccagtgc acccgatccg ggcggggcgt
     2161 gacatggacg gtggagccgg gttgcgggat ggccgtgtgc tcctccgcgc ggacgatgac
     2221 ctgctcggcg ccgaggcgac cgtagatcag gcgctccgcg cccagcagct cggtggtttc
     2281 gactaccagg ggccagccgc tgggggtgac gtccaggtgc tcggggcgca cgcccatgat
     2341 cgtgcccgcg cgcgatccgg gcgcacccgt cagcaggttc atgggcggcg agccgatgaa
     2401 gctggccacg aacgtggaag ccggctggtg gtacacctct tccggtgtgc cgaactgttc
     2461 catcctgccg gcattcatca ccagcatgcg ttgggccaag gtcatggcct cgacctggtc
     2521 gtgcgtcacg aacagcgacg tgatgcccag ctcgcggtgc agcttctgga tctcgatgcg
     2581 ggtctgggcc cgcagcttgg cgtccaggtt ggacagcggt tcgtcgaaca ggaacacctg
     2641 gggctggcgc acgatggcgc ggcccatggc cacccgttgg cgctggccac cggagagctg
     2701 gcgcggcttg cgctccagca gctgccccag ctcgagaatg ccagcggcct tgtcgacacg
     2761 cgtcttgatc tcctggggtg gcaccttctt gatcttgagg ccataggcca tgttgtcgaa
     2821 tacgctcatg tgcgggtaga gcgcgtagtt ctggaagacc atggcgatgt cgcggtcgga
     2881 gggctccagg ttgttgacga cccggttgcc gattgaaatc tcgccttcgg taatttcctc
     2941 gaggccggcg accatccgca gcagcgtgga ctttccgcat cccgaagggc cgacgatgac
     3001 gacgaattcg cggtcttcga ccatggcgtc gacgccgtgg ataacctgca gctcgtgttt
     3061 gcccgagccg tagcgcttga cgatgttctt gagtgttatc gatgccatgg caaggagagt
     3121 ttccgaacgg agagggtgaa ttccttcatt tctcggtgtc caccaagccc ttgacgaacc
     3181 atttttgcat cgcaagcacc acgaccgctg gcgggatcat tgccaggata gccgtggcca
     3241 tgacgatgtt ccactcgttg gccgcgtcgc cccccgagat catgcgcttg atgcccatca
     3301 ccacgggata catggattcg tcggtggtca cgagcagcgg ccacaggtac tgattccagc
     3361 cgtagatgaa ctggatgacg aaaagcgcgg cgatgctggt ggttgacagc ggcaccaaca
     3421 cgtccttgaa gaagcgcagc ggccccgcgc cgtccacccg ggccgcctcg acgagttcgt
     3481 ccggcaccgt caggaaaaac tgccggaaca ggaaagtggc cgtggcgctt gcgatcagcg
     3541 gcaccgtcag gccggcatag ctgttgagca tgccgaggtc ggagaccacc tggtaagtgg
     3601 gaccgatgcg cacctccacc ggcaacatca gcgtgacgaa aatcgcccag aagaagaaca
     3661 tgcgcccggg gaagcggaaa tagacgattg cgaacgccga gagcagtgaa atcgcgatct
     3721 tgccgatggc gatgaccagc gccgtgatca agctcactgt catcatgcgt cccacgggcg
     3781 cggcggaagc ggctgcactg gtcgaaccct tgatggcggc gatgtagttg tccaccaggt
     3841 gcggccccgg tagcaaaggc atcggcacct gcacaatgtc ctgggcggtg tgcgtgcttg
     3901 ctataaaggt cacgtacaag ggaaaggcga taacaatcac accgagcacg agcacgaagt
     3961 gtgagatggc cgtggcaagc gggcggcgtt cgaccatcag taattcacct ttctctcgac
     4021 gtagcggaac tgcaccacgg tcaaggcaat gacgatgacc atcagcacga cggactgcgc
     4081 cgccgaacca ccgagatcca gcgccttgaa accgtcgtaa tagaccttgt aaaccaggat
     4141 tgccgtgtcc ttgcccgggc ctccttccgt ggcggcgtcg acg
//
LOCUS       DASWIY010000344         4765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_225003, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000344 DASWIY010000000
VERSION     DASWIY010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225003"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..810
                     /locus_tag="VGA59_12300"
     CDS             196..810
                     /locus_tag="VGA59_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9720504.1"
                     /translation="MNLCSFSRRAMVLSKDNPTPHLGFLVEGRLQGVDFTVDGRSVGL
                     YFVDPGDYFGELSVVDGKPPSEFVVAAAKSTVALLEARTARELISVHPALASAVMAKL
                     AQRVRLVTAQRTLLGLPNPFQRLCSLLLQLPQSQADGQAAVDNAPTHQEMAIMINASR
                     ETVTRAFQVLFIKKLLVREGNMLKLLRSDTLRDIAEGRAEPPKA"
     gene            838..1641
                     /locus_tag="VGA59_12305"
     CDS             838..1641
                     /locus_tag="VGA59_12305"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9720505.1"
                     /translation="MTSLLSYLIPLRHAGLIRQFANREVHSRYRQSWLGAFWALLTPL
                     LMLGVYTFVFRYVFGVRWGVTVESNLSFAVRLYAGLAVFNFFAECVARAPRLVLEQPH
                     LVKKVVFPLEVLPWVNAMAGLVHLGIALAMLLGLATWETGRIHLSVLALPLVWLPLVP
                     LTVGLGWWLSAIGAFVRDVGQVISMLLSLMLFLSPIFFPVEALPKAWQSWMFLNPLAL
                     VITETRHVLIDGLWPDWPALALNLGACVLVAVAGAAFFRSARTGFADVV"
     gene            1631..2854
                     /locus_tag="VGA59_12310"
     CDS             1631..2854
                     /locus_tag="VGA59_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720506.1"
                     /translation="MSSEPTQGLVIEARRLGKCYSLYERPADRLKQLLWGRWHRYDRE
                     FWALRDIDLSIRAGEVIGVVGRNGAGKSTLLQLVCGTLPPTAGELHVRGRVAPLLELG
                     AGFSPDFTGIENIYMNAAILGLKRHEVDERLADIVAFADIGDFVKQPVKTYSTGMYMR
                     LAFAVATAVEPDILVIDEALSVGDGAFARKSFDRIMKLREAGKTILFCSHSMYQVEAL
                     CAKAMWIESGALRMFGSSAEVTSAYQASLNATTLPREVRTPAAAAALPAPAGTGRILK
                     VSASADGATGNELKLRSGESDLSITVEFSIDPNLPTPAVAVGICDSNGLTVASATSHD
                     DGVTLQTDAGGRGSASVVFAQLPLLKGQYSVSCFLVSQDGVHPYEQVEPKLVLHVTQK
                     GVEQGLVTLPHEWMQ"
     gene            2851..3873
                     /locus_tag="VGA59_12315"
     CDS             2851..3873
                     /locus_tag="VGA59_12315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9720507.1"
                     /translation="MNAEPLVNGTARQVEIGGALFQVRDLSPADEDSLLRLHVHVFGP
                     GATQAWYQWKYRVGGGLGSGVWHQGELIAHCGGVPRTLWRQGRRQTGLQIGDVMVAPE
                     WRGILTRRGPFFQASHAFYSAHVGDRFGHSIAYGFPSERHLRLAVTLKLLWSAGPVHA
                     LSWDVQAADRSGPGWAWSWHCLDPSDRQFDQSVDAAWQRMRAAGAALTFGERDAAYVR
                     WRYCERPDRRCSFFSLRRPWSPTPAGIAVLDIGASPVQWLDWIGDPAAILAASRACVA
                     EAGRAGAPSLTAWASPVVTQSLQGAEVTSKSVTAWLGIPRASSLAEEDLAGMRWWLMG
                     GDTDFL"
     gene            3870..4625
                     /locus_tag="VGA59_12320"
     CDS             3870..4625
                     /locus_tag="VGA59_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9720508.1"
                     /translation="MTNTADIKPAGELVFVTGVSRLDVLGRYLCQSPCIKSGRRRLVA
                     YFNTSSAANAFNAAMDGIPADASSTWLVWVHQDVLLPDNWDERFLDALRMAQQENPAI
                     AVAGVYGVAGAPANARRAGHVLDRGSLLQEPARLPCAVDSLDELLFAVRADTRLKLDP
                     AMGFDFYATDLVLEAQARGWVAVVVDAYCEHWSDTPAAGAIPDRIADRIIRNGRAFES
                     KWSHRFPIQTSWVAVQAPGDVEQFIAALRHRQQ"
BASE COUNT          764 a         1534 c         1585 g          882 t
ORIGIN      
        1 ggcccgcaat gacaaccgtc acggtcaagc ccgcaatgac agccttttca gcccccgcat
       61 gggaacgtcc agcactgccg cgcagactcc gagcctttaa actcgggccg aatgccggtc
      121 tccctccaac tgcttcacca gctgcccctg ctgcgcccat tgcccagcga agtactagag
      181 actctgtcgg gagccatgaa cctgtgctcc ttctcgcggc gggcgatggt cttgtccaag
      241 gacaacccca caccgcatct gggcttcctg gtcgaggggc ggctgcaggg tgtggacttc
      301 acggtggacg ggcgcagcgt cggcctctat ttcgtcgatc cgggcgatta tttcggcgaa
      361 ctgtccgtgg tggacggcaa gccgccgtcg gagttcgtgg tggcagcggc aaaatccacg
      421 gtcgcgctgc tggaagccag gacggccagg gaactgatct ccgtacaccc ggcgctggcc
      481 agcgccgtga tggcaaagct cgcccagcgc gtgcggctcg tgaccgcgca gcggacactg
      541 ctgggactgc ccaatccgtt ccagaggctg tgctccctgc tcttacaatt gccgcagagt
      601 caggcggacg ggcaggcggc cgtcgacaat gcgccgacgc accaggaaat ggcgatcatg
      661 atcaacgcga gccgggagac ggtgacgcgg gcgttccagg tcctgttcat caagaagctg
      721 ctggtccgtg agggcaacat gctcaagctg ctgcgctccg acacattgcg cgacatcgcc
      781 gaaggccgtg ccgaaccccc caaggcttga ccccctgaaa ccggctcatt tcccaacgtg
      841 acatccttgc tgtcgtacct gataccgctg cgccatgccg gcctgatccg gcaattcgcc
      901 aaccgggagg ttcattcccg ctatcgccag tcctggctgg gcgctttctg ggcgctgctg
      961 acgccgctgc tgatgctggg tgtgtatacg ttcgtgttcc gctacgtgtt cggagtgcgt
     1021 tggggcgtca ccgtcgaaag caacctcagc ttcgccgtgc gcctgtacgc cggcctggcg
     1081 gtgttcaact tcttcgccga atgcgtagcc cgcgcgcccc ggctggtgct tgaacagccg
     1141 catctggtca agaaggtcgt gttcccgctc gaggtgctgc cctgggtcaa tgccatggcc
     1201 ggcctggtcc acctcggcat cgccctggcg atgctgctgg ggctggccac gtgggaaacg
     1261 ggccgcatcc acctgtcggt gctggcgttg ccgctggtgt ggttgcccct ggttcccctg
     1321 accgtgggcc tgggttggtg gctctccgcg atcggcgcct ttgtccgcga cgtcggccag
     1381 gtgatcagca tgctcttgag cctgatgctc tttctcagcc ctattttctt cccggtggag
     1441 gcgttgccca aagcgtggca atcgtggatg ttcctgaacc cgctggcgct ggtcattacc
     1501 gagacccggc acgtcctgat cgatggactg tggcccgact ggccggcgct cgcgctgaac
     1561 ctcggcgcct gcgtcctggt ggccgtggcg ggcgccgcct tcttccgttc ggcccgcacg
     1621 gggttcgccg atgtcgtctg agccgacaca gggcctcgtg atcgaggccc gtcgcctggg
     1681 taaatgctat tcgctgtacg aacgcccggc cgaccgactc aagcagctgc tgtgggggcg
     1741 ctggcaccgg tatgaccgcg agttctgggc cttgcgcgac atcgacctct cgatccgggc
     1801 gggcgaagtc atcggtgtgg tcggccgcaa cggggcgggc aagtccaccc tgctgcaact
     1861 ggtgtgcggg accttgccgc ccacggccgg ggagcttcac gtgcggggtc gcgtcgcgcc
     1921 gctgctggag ctgggtgccg ggttcagccc cgacttcacc ggaatcgaga acatctacat
     1981 gaacgcggcg atcctgggac tcaagcgcca cgaggtggac gaacgcctgg ccgacatcgt
     2041 ggcgtttgcc gatatcggag acttcgtcaa gcagccggtc aagacctatt ccaccggcat
     2101 gtacatgcgc ctggcgttcg ccgtcgcgac cgcggtcgag cccgacatcc tggtcatcga
     2161 cgaggcactg tcggtgggcg acggcgcttt cgcgcgaaag tcgttcgacc gcatcatgaa
     2221 gctcagggag gcaggcaaga cgatcctgtt ctgctcgcac tcgatgtacc aggtcgaagc
     2281 actgtgcgcc aaggccatgt ggattgaaag cggcgcgttg cggatgttcg ggtcatcggc
     2341 cgaagtcacc agcgcctacc aggccagcct caatgcgacc acattgccca gggaggttcg
     2401 cacccccgcg gccgcggccg cgctgccggc cccggcgggt accggacgga tcttgaaggt
     2461 gagcgcgagc gcggacggcg cgaccggaaa cgaactgaag ctgcgcagcg gtgaaagcga
     2521 cctgagcatc accgtcgagt tcagcatcga cccgaacctg ccgacgcctg ccgtggcagt
     2581 gggtatctgc gacagcaacg gcctcacggt agccagcgcg acatcgcacg acgatggcgt
     2641 caccctgcaa acagacgcgg gcgggcgcgg cagtgcctcg gtcgtgttcg cgcaacttcc
     2701 gctgctcaag ggccagtact ccgtcagctg ctttctggtg tcgcaggacg gtgtccatcc
     2761 ctatgaacag gtggagccca aactcgtgtt gcacgtgacg cagaaaggcg tcgaacaagg
     2821 gttggtgacc ctgccgcacg aatggatgca atgaacgcgg aaccgctcgt gaacggcacg
     2881 gcccggcagg tggaaatcgg cggagcactg ttccaggtgc gcgatctgtc tcccgcggat
     2941 gaagacagcc tcttgcggct gcacgtgcac gtgttcggcc ccggcgcgac ccaggcgtgg
     3001 taccagtgga aataccgggt gggcggcggc cttggcagcg gtgtgtggca ccagggcgag
     3061 ctgatcgccc attgcggcgg cgtgccacgc actctctggc gccaggggcg gcggcaaacc
     3121 ggccttcaga tcggggacgt gatggtcgcg ccggaatggc gaggcatcct gacgcgccgc
     3181 gggccgttct ttcaagcgtc ccatgctttc tattcggctc atgtcggcga ccgattcggc
     3241 cattcgatcg cctatgggtt tcccagcgag cggcacttgc gccttgcggt gacgctcaag
     3301 ctgctgtgga gtgcggggcc tgtccatgcc ctgagctggg atgttcaggc agcagatcga
     3361 agcgggccag gctgggcctg gagctggcat tgcctggatc cctccgaccg ccaattcgat
     3421 caatccgtag acgcggcctg gcagcgtatg cgcgcagccg gcgccgcact gacgttcgga
     3481 gagcgcgatg ccgcttacgt gcgatggcgc tattgcgagc ggccggaccg gcgctgcagc
     3541 ttcttttcgc ttcgcaggcc ctggtcgccc acacccgcgg ggatcgccgt gctggacatc
     3601 ggggcttccc cggtgcagtg gctggactgg attggggacc ctgcggccat ccttgccgcg
     3661 agccgcgcgt gcgtcgccga ggctgggcgc gccggcgcac ccagtctgac cgcgtgggcc
     3721 agcccggtag tgacacaatc cctgcaaggt gcagaagtca ccagcaagag cgtcaccgca
     3781 tggcttggca tcccacgcgc atcgtccctg gcggaggaag acctggccgg catgcgttgg
     3841 tggctcatgg gtggggatac cgacttctta tgaccaatac agccgatatc aaacccgctg
     3901 gagaactggt gttcgtcacc ggcgtgagcc ggcttgacgt gctcggtcgc tacctgtgcc
     3961 aatcaccttg catcaagtca gggcgacgcc ggctcgtcgc gtacttcaat acttcgtccg
     4021 cggcgaatgc cttcaacgcc gcgatggacg gcatcccggc cgatgcgagt tcgacctggc
     4081 tggtctgggt gcatcaggac gtattgctgc cggacaactg ggacgagcgt tttctcgatg
     4141 cgctgcgaat ggcccaacag gagaacccgg cgatcgccgt ggccggagtc tacggcgtgg
     4201 caggtgcgcc tgcgaacgca cgccgcgcag ggcacgtcct ggatcgtggc tcgctcctgc
     4261 aagagcctgc gcggctgcct tgcgccgtcg acagtctgga cgaattgctg tttgcggtgc
     4321 gcgccgatac gcggctgaag ctcgaccctg ccatgggctt cgacttttac gcgacggacc
     4381 tggttctcga ggcccaggcg cgcggatggg ttgcggtggt tgtcgatgct tattgcgagc
     4441 actggtcgga cacgcctgcg gccggcgcga ttccggatcg catcgcagac aggatcatcc
     4501 gcaatggcag ggcgttcgag tcgaagtggt cgcaccggtt tccgatccag acatcctggg
     4561 tggcggtgca agcgccgggt gatgtcgagc aattcattgc cgcattgcgt catcgacagc
     4621 aatgatcacg gcgccacagc tcgcccggga cagcgagagc ctgcagaccg ggagacttga
     4681 tgtcctcgcc accctgcgtt gacatcgcga tcgtcaatta cttcagcgcc ccggacgtgc
     4741 ggcgctgcct ggaacggctg gggcc
//
LOCUS       DASWIY010000345         1875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_602755, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000345 DASWIY010000000
VERSION     DASWIY010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602755"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..774
                     /locus_tag="VGA59_12325"
     CDS             211..774
                     /locus_tag="VGA59_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720509.1"
                     /translation="MPARAHAMDGELAHLACPNPDCALFNRFNAGNLSVCERIGKHKR
                     IRRLYCSHCGGRFSERRGSLMQYTKLPEPAVVRIIKCLSHGCSIEATADICQVDPRSV
                     RRMLEKAGTRAEDFHQFQVERLEHPPAVVELDELHGRVCKPPPSARKKGRPARRRTGG
                     GALDAIAAWADGGFTWPWRPQHASSWT"
     gene            735..1475
                     /locus_tag="VGA59_12330"
     CDS             735..1475
                     /locus_tag="VGA59_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720510.1"
                     /translation="MALATAARFILDLRIGPRTIETATELLTSVAMACGAGGGAGGAS
                     PPLLLIDEHLPYPAAILQVFGVVRHRRRRRKRGRRKKPDLKPPPGLLAGVVGKVRDAT
                     GNLLRVRTRALFGRKRDIIGRIRQLGLGQQINTAHIERLNGTMRGQQARLARRTRAGS
                     RRGRTLRWSTALWRDVYNWVRVHASLAGSTPAMAIGLADAPWSVSRYVGHPVHVSDLQ
                     RAIWAEERETWRTSALDAREHRKLLPAS"
     gene            1622..>1875
                     /locus_tag="VGA59_12335"
     CDS             1622..>1875
                     /locus_tag="VGA59_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720511.1"
                     /translation="MAGKTYVVVKSMTVKGGGGVPKEFVKDAPKIMTKAALSALKSAS
                     TEGDDKGDKKRKGFSITAVLNKVDIDPKAKSVTCDVSGYVS"
BASE COUNT          315 a          613 c          676 g          271 t
ORIGIN      
        1 actcggcttt gcgagaagca cgcgggagtc gttgcgcgac cgtgagcgcc cgtcgcgtgc
       61 ctccgaatac ccgggtatcc gggtatccgg gtatccgggt attcggtagt gcctcacgat
      121 gttggcagac aatcgcggtg aatttgggcc tgaaggcggg cggttgtcga tgaatcgggc
      181 agacgaccca ttctggctgg gaggatcccc atgcccgccc gcgcgcatgc catggacggc
      241 gaactggcgc atctggcctg ccccaatccc gactgcgccc tgttcaatcg cttcaacgcc
      301 ggcaacctca gcgtctgcga acgcatcggc aagcacaaac gcatccgccg gctgtactgc
      361 agccactgcg gcgggcgctt cagcgaacgc cgcggctcgc tcatgcaata tacgaagctg
      421 cccgagccgg cggtggtgcg catcatcaag tgcctgtccc acggttgctc catcgaggcc
      481 acggccgaca tctgccaggt cgatccgcgc agcgtccggc ggatgctgga gaaggccggc
      541 acgcgggccg aggacttcca ccagttccag gtggagcgcc tggagcatcc gccggcggtg
      601 gtcgaactcg acgagctgca cggccgggtg tgcaagccgc cgccgtcggc gcgaaaaaag
      661 gggaggccgg cgcggcggcg aacgggcggg ggagcgctgg acgccatcgc ggcctgggcc
      721 gatggtgggt tcacgtggcc ctggcgaccg cagcacgctt catcctggac ctgaggatcg
      781 ggccgcgcac gatcgagacc gccacggaat tgctgacctc ggtggcgatg gcctgcgggg
      841 ccgggggcgg ggccgggggc gcttcgccgc cgctgctgct gatcgacgag cacctgccct
      901 atccggcggc gatcctccag gtcttcggcg tggtccggca tcgccgacgg cgacgcaagc
      961 gcggccggcg caagaagccg gacctcaagc ccccgcccgg cctgctggcc ggcgtggtgg
     1021 gcaaggtccg cgacgccacg ggcaacctgc tgcgggtgcg gacccgggcc ctcttcggcc
     1081 gcaagcgcga catcatcggc cgcatccggc aactgggcct cggccagcag atcaacaccg
     1141 cgcacatcga gcggctcaac gggaccatgc gcggccagca ggcgcggctg gcgcggcgca
     1201 cccgggccgg ctcgcgtcgg ggccggacgc tgaggtggtc gacggcgttg tggcgcgacg
     1261 tgtacaactg ggtgcgggtg catgcgtcgc tggcggggag cacgccggcg atggccatag
     1321 ggctggccga cgcgccctgg tcggtgtcgc gctacgtcgg gcaccccgtg cacgtcagcg
     1381 acctccagcg ggcgatctgg gccgaagagc gcgaaacctg gcgaacctca gccttggacg
     1441 cccgagaaca tcgaaaactc ttaccagcat cgtgaggcac taccgggtat tcgggtattc
     1501 gggtatccgg gggcgccggt agcgcgccac ccggttggcc catgtcgtga gggacgccgg
     1561 ccccgattgc gtactttcag aggtacactg ccgcactcgc actcgcacaa ggagcaatca
     1621 catggccggc aagacctacg tggtggtgaa gtcgatgacc gtcaagggcg gcgggggcgt
     1681 gcccaaggag ttcgtcaagg acgccccgaa gatcatgacc aaggcggcgc tctcggccct
     1741 gaagtcggcg agcaccgagg gggatgacaa gggggacaag aagcgcaagg gtttctcgat
     1801 caccgcggtg ctgaacaagg tggacatcga cccgaaggcc aagagcgtca cgtgcgatgt
     1861 cagcgggtat gtctc
//
LOCUS       DASWIY010000346         5325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2092825, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000346 DASWIY010000000
VERSION     DASWIY010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092825"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1108
                     /locus_tag="VGA59_12340"
     CDS             119..1108
                     /locus_tag="VGA59_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9720512.1"
                     /translation="MSKAFASQADLADKKITFEQLSAHCWAYTAEGDPNSGVIMGDKF
                     IMVSDATATPAMAQDLIARIREVSNKPIKYVLLTHYHAVRVLGASAYAAEGATEIIAS
                     QGTLDLIVERGRQDMQSEMERFPRLFRNAESVPGLTWPTLVIGGGNPAKGEVPGRLTV
                     DLGGVQVQIWHPGAGHTRGDTIAWVEDEKVLFSGDLVEYEAGVYTGDAHLQEWPATLE
                     ALRALRAEALVPGRGQAMKGAADVNKALDYTKRWVETLYAAAREAAAANMDLKAAMAH
                     TRKLMDPVFGHVFIYEHCLPFDVSRAYDEARGIKNPRIWTAERDKEMWDALQA"
     gene            1433..1744
                     /locus_tag="VGA59_12345"
     CDS             1433..1744
                     /locus_tag="VGA59_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720513.1"
                     /translation="MPSNDKSVPRILQQQPPRDSDPEQQQSGSNEFQPKADEPKEGRQ
                     QMGEGSYEATRDYQKNIKEYLKKADVEADAQAARPRSEQEAKDLDEAEKEGRSHSKGE
                     R"
     gene            complement(1749..2897)
                     /locus_tag="VGA59_12350"
     CDS             complement(1749..2897)
                     /locus_tag="VGA59_12350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9720514.1"
                     /translation="MTAVSAMSVIWRLAAATTLIMLAFAMAAPVLAISLQQAGYSTAA
                     VGAFAMIPFLLIALLIPIVPRMLARWGIVRTYRWGCFLQLGGALGYATGDGFAVWSAA
                     SIACGTGAAALWNATEALLAREAPPERRGRVMGLYQTSLGAALALGPFMPALLGWSAR
                     PVLWLAAALVAVCCVMALAIPRHATTEPAPHTQRGTWHALRTVPALAGIAFTGGVFEA
                     GLGSISAAYASATGMGLSMAATVAGAIGVGSFLCQYPAGAAADRFKASTVFSAAALLL
                     LAASLAFALAGRAPWLLWGVGLVWGGVGGALYTLTMVGVAREFSGRATAGGAAAMITG
                     YTLGGTVGPLASGSALQWGGVGGLAAVLGSLALATLWAARGIGRPRTS"
     gene            complement(2894..3766)
                     /locus_tag="VGA59_12355"
     CDS             complement(2894..3766)
                     /locus_tag="VGA59_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HEX9720515.1"
                     /translation="MRIVSILGRIIGLLALATVLGACSAVKLGYNNLGEVAYWWLDSY
                     VDFEGEQAARVREDLARLHLWHRSRELPRLAQVLQHLEELAPGDITAAQACVVVQQVR
                     ERLNATAEHAVPAMVTRALDLAPEQLLHLERKYETNNAKYRKEWLRATAAERAEKRFR
                     QFLERSETIYGDLDGAQRAVLRRQLEQSIFDADRILAERRRRQRDALATLRKLAGQPV
                     SFDEARALLRGYLARIEEPPDPRQRRYLKALIEEGCRTFAGLHNSTSAAQRQAAVRRL
                     RTYQRDLRELAAQQ"
     gene            3784..5184
                     /gene="glmU"
                     /locus_tag="VGA59_12360"
     CDS             3784..5184
                     /gene="glmU"
                     /locus_tag="VGA59_12360"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX9720516.1"
                     /translation="MTAVDVVIMAAGLGTRMKSRLPKVLHRLAGRALVRHVADSAAAL
                     RARRTVVVTGSGAAEVETELTRWAQDRGMTVPAFVRQEPQLGTGHAVQQAVPLLPDEG
                     TTLILNGDVPLIEAATLQSLVERGGGRALALLSVEMPDPTGYGRILRTGDAVAAIVEH
                     KDATEEQRRVTEVYTGFMAVPNSMLKRWLSRLTNDNAQGEYYLTDIVRLAIQDGVDVV
                     AVRTDDKVQVDGVNSPAQLAALERAFQLRQAQALMEQGVRIADPARFDVRGELACAQD
                     VEIDVNCVFEGRVSLGEGVRIGANCVIANADISAGAVIHPFTHIDGEKSGVRVGEGAL
                     VGPFARLRPGADLGPEVHVGNFVEIKNSTLARGAKANHLAYLGDATVGARVNYGAGSI
                     TANYDGANKHRTVIEEDVHVGSNCVLVAPVTIGRGGTVGASSAITKDTPPGALSVARG
                     KQVSIANWQRPKKTGK"
     gene            complement(5229..>5325)
                     /locus_tag="VGA59_12365"
     CDS             complement(5229..>5325)
                     /locus_tag="VGA59_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9720517.1"
                     /translation="TSDANVRNVKAFFSLHRAKFQPRIPLAGRRA"
BASE COUNT          967 a         1770 c         1826 g          762 t
ORIGIN      
        1 cgtaactgat cagggcctgt tcacactgtt ttgcctggca aaaacagtgt gaacaggcca
       61 taggcttggg taagatcatc ggcttcccgt ttttgcgtcc gcgcaaagga gagccgagat
      121 gtccaaagcc tttgccagcc aggccgacct ggccgacaag aaaatcacct tcgagcagct
      181 cagtgcccat tgctgggcct acaccgccga gggcgacccg aactcgggcg tcatcatggg
      241 cgacaagttc atcatggtca gcgatgccac cgccaccccc gcgatggcgc aggacctgat
      301 cgcccgcatc cgcgaggtga gcaacaagcc gatcaagtac gtgctgctca cgcattacca
      361 cgcggtgcgc gtgctgggcg cgagcgcgta tgcggccgaa ggcgccaccg aaatcatcgc
      421 gagccagggc acgctggacc tgatcgtcga gcgcggcagg caggacatgc agtccgagat
      481 ggagcgcttc ccgcgcctgt tccgcaatgc cgagagcgtg cccggcctga cctggccgac
      541 cctggtgatc ggcggcggca atccggccaa aggcgaggtt ccgggccggc tcacggtgga
      601 cctgggcggg gtccaggtgc agatctggca tcccggcgcc ggccacacgc gcggcgacac
      661 cattgcctgg gtcgaggacg aaaaggtgct gttttccggc gacctggtcg aatacgaagc
      721 cggggtctac accggcgacg cgcacctgca ggaatggccg gccacgctcg aagccttgcg
      781 cgcgttgcgc gccgaggccc tcgttcccgg ccgcggccag gcgatgaagg gagccgccga
      841 cgtcaacaag gccctggact acaccaaacg ctgggtcgag acgctgtacg cggcggcccg
      901 ggaggcggct gccgccaaca tggacctcaa ggcggcaatg gcccataccc gcaaactgat
      961 ggacccggtg ttcggccatg tgttcatcta cgagcattgc ctgccattcg acgtgagccg
     1021 cgcctacgac gaagcgcgcg gcatcaagaa cccgcggatc tggaccgccg agcgcgacaa
     1081 ggagatgtgg gacgcgttgc aggcttgagc atgcgcgcga agtgatgtag gccggcaccc
     1141 acaagcctgc cgcatgacgg acgacagagc cgctgggctc gcactcctaa gctgcaacat
     1201 tcgttttgtc atcccgggca gagtcttgaa tttgtcatcc cggacttgac cctgaacttg
     1261 tcatcccgga ccccggatca agtccggggc aggcttgatc cgggatccat gaattcatgg
     1321 attgcgggtc aggcccgcaa tgacaacgtt ttgggtccag gtccgcaatg acaaggctgc
     1381 gggtccaggc ccgcaatgac aaagatcggt gacacagcca caggagtgat ccatgccttc
     1441 caacgacaag tccgtgccgc gcatcctgca gcaacaaccg ccgcgggaca gcgaccccga
     1501 gcagcaacaa tccggctcga acgagtttca gcccaaggcg gacgagccga aagagggccg
     1561 gcagcagatg ggcgagggca gctacgaagc gacccgcgac taccagaaga acatcaagga
     1621 gtacctgaag aaggccgacg tggaggcgga cgcgcaagcg gccaggccgc ggtcggagca
     1681 ggaagccaaa gatctcgacg aggctgaaaa agaaggccgc tcgcactcca agggcgagcg
     1741 ctagtcagtc agcttgtccg tggccggccg atgccgcgcg cggcccagag ggtcgccagg
     1801 gcgagcgagc cgagcactgc cgccaggccg ccgacgccgc cccattgcag ggcgctgccg
     1861 cttgccaatg gcccgacggt gcctcccagc gtatagccgg tgatcatcgc ggccgcgccg
     1921 cccgccgtgg cacggcccga aaattcgcgc gccacgccca ccatggtcag cgtgtacagc
     1981 gcgccgccga ccccgcccca gaccagcccc acgccccaca gcagccaggg cgcgcggcct
     2041 gccaaagcga atgccaggct ggcagccagc aacagcagcg ctgctgccga aaacaccgtc
     2101 gatgccttga agcggtcagc cgcggcgccg gccgggtact ggcaaagaaa gctgcccacc
     2161 ccgatggcgc cggccacggt tgccgccatg ctcaagccca ttccagtggc cgacgcatag
     2221 gcggcgctga tggagccgag cccggcttcg aacacgccgc cggtgaaggc gatgcccgcc
     2281 agcgcaggca ccgttcgcaa ggcgtgccag gtcccgcgct gggtatgcgg cgccggctcg
     2341 gtcgtcgcat gccgcggtat ggccagcgcc atcacgcagc agacggcgac cagcgcggca
     2401 gccaaccaca acacgggccg cgcgctccac cccagcagcg ccggcatgaa aggaccgagc
     2461 gccagcgccg ccccgagcga ggtctggtac aggcccatga cccggccgcg ccgctcgggc
     2521 ggcgcttcgc gagccagcag cgcttcggtg gcgttccaga gtgctgccgc gcccgtgccg
     2581 caggcgatcg aggccgccga ccacaccgcg aagccatcgc cggtcgcgta accgagcgca
     2641 ccgcccaact gcaggaagca gccccagcgg tatgtccgaa cgatgcccca gcgggccagc
     2701 atacggggga cgatggggat cagcagggcg atgagcagga acggaatcat cgcgaaggcg
     2761 ccgacagcgg cagtgctgta gccggcctgc tgcaggctga tcgcgagcac gggtgcggcc
     2821 atcgcgaacg ccagcatgat cagcgtggtc gccgcggcca gccgccagat caccgacatc
     2881 gccgatacgg cagtcattgc tgcgcggcga gttcgcgcag gtcgcgctgg taggtgcgca
     2941 gccgccgcac cgccgcttga cgctgggccg cgctggtgct gttgtgcagg ccggcgaaag
     3001 tgcggcatcc ctcttcgatc agcgccttca gatagcggcg ctgacggggg tcgggcggct
     3061 cctcgattcg cgcgagatag ccgcgcagca aggcgcgcgc ctcgtcgaag gacaccggct
     3121 ggccggccag tttgcgcagg gttgcgaggg cgtcacgctg ccggcgacgg cgttcggcga
     3181 ggatgcggtc cgcatcgaaa atcgattgct cgagctggcg gcgcaagacc gcgcgctgcg
     3241 ccccgtccag gtcgccatag atggtttcac tgcgctccag gaactgcctg aaacgcttct
     3301 ccgcccgttc ggcggcggtc gcgcgcagcc attccttgcg gtacttggca ttgttcgttt
     3361 cgtacttgcg ttcaaggtgc agcaattgct cgggcgcgag gtcgagggct cgggtgacca
     3421 tcgcgggcac cgcgtgctcg gccgtggcgt tgaggcgctc gcgcacctgc tgcacgacca
     3481 cgcaggcttg ggcggcggtg atgtcgccgg gcgccagttc ctccaagtgc tgcagtacct
     3541 gggccagccg tggcagttcc cggctgcggt gccacaagtg cagccgcgcg aggtcctcgc
     3601 gcacgcgtgc cgcctgctcc ccttcgaagt cgacgtagct gtccagccac cagtaggcga
     3661 cctcgccgag attgttgtag cccagcttca cggcgctgca ggcgcccaga acggtggcca
     3721 gggccagcag accgataatc cggccaagta ttgaaacgat acgcatctgt acaaggaaat
     3781 tgcatgacag cagtcgatgt ggtcatcatg gcggcgggcc tgggcacgcg catgaagagc
     3841 cgcttgccca aagtgttgca ccgattggcc ggccgtgcat tggtgcggca cgtggccgat
     3901 tcggcggcgg ccctgcgagc gcgccgcacc gtcgtggtca cgggcagtgg cgcggccgag
     3961 gtggaaacgg agctgacgcg ctgggcgcag gatcgcggca tgaccgtgcc ggcgttcgtg
     4021 cgccaggagc cccagctcgg caccggccat gcggtgcagc aggccgttcc gctgttgccg
     4081 gacgaaggga cgaccctgat cctgaatggc gacgttccgc tgatcgaggc ggccacgctg
     4141 caaagcctgg tcgagcgcgg cggcggccgc gccctggcac tgttgagcgt ggagatgccc
     4201 gaccccaccg gctacggccg catcctgcgc accggcgacg ccgtggcggc gatcgtggaa
     4261 cacaaggacg ccaccgagga gcagcgccgc gtcactgagg tctacaccgg tttcatggcc
     4321 gtccccaaca gcatgctcaa gcgctggctg tcccgactga ccaatgacaa tgcgcaaggc
     4381 gagtactacc tcacggacat cgtccggctg gccatccagg acggcgtcga tgtggtggcc
     4441 gtcaggaccg atgacaaggt gcaggtcgat ggcgtcaaca gtccggcgca gctggccgcc
     4501 ctggagcgcg ccttccagct gcgccaggcc caagccttga tggagcaggg cgtgcgaatc
     4561 gccgacccgg cacggttcga tgtgcgcggc gagctcgcct gcgcgcaaga cgtggagatc
     4621 gacgtcaatt gcgtgttcga gggccgtgtt tcgctggggg aaggcgtgcg catcggtgcc
     4681 aactgcgtga tcgccaacgc cgacatttcg gccggtgcgg tgatccatcc attcacccat
     4741 atcgacggcg agaaatcggg cgttcgcgtc ggcgagggcg ccctggtcgg ccctttcgcg
     4801 cggctgcgtc cgggcgcgga cctggggccg gaggtgcatg tgggcaactt cgtggagatc
     4861 aagaattcga ccctggcacg cggtgccaag gccaaccacc tggcctatct gggcgatgcc
     4921 acggtgggcg cgcgtgtgaa ctacggcgcg ggctccatca ccgccaacta cgacggcgcc
     4981 aacaagcacc gcaccgtgat cgaggaggac gtgcatgtcg gcagcaactg cgtgctggtg
     5041 gcacccgtga ccatcggccg gggcggcacg gtgggagcca gttccgccat caccaaggac
     5101 acgccgcccg gggcgctgtc ggtggcgcgc ggcaagcagg tgagcatcgc gaactggcag
     5161 cggccgaaaa agaccggcaa ataggccgca agaagggcgc ggcggaccgc ttgtgcgttc
     5221 gggcgacctc aggcccggcg gcccgccagc ggaattcgcg gctggaactt tgcccggtgc
     5281 agcgagaaga aggctttgac attgcggaca ttggcgtcgc tggtg
//
LOCUS       DASWIY010000347         6240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1723184, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000347 DASWIY010000000
VERSION     DASWIY010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723184"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2111
                     /gene="rocD"
                     /locus_tag="VGA59_12370"
     CDS             <1..2111
                     /gene="rocD"
                     /locus_tag="VGA59_12370"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEX9720518.1"
                     /translation="VPVVVDHARDCLVWDVEGREYIDMMSAYSAVSHGHLHPRLVAAA
                     HRQLERVAVTSRAYHGTTLGPFLEKLCRLAGFERALPMNTGAEAVETAIKCARRWGYR
                     IKGIPDGRAEILVARGNFHGRTSTIIGFSSEPEYRAGFGPFAPGFVHFDFGDMASVRA
                     ATSANTCAVLVEPIQGEAGVIVPPAGFFAELREWCDRNNVLLIIDEVQAGLGRTGRWF
                     AFEHEGIRPDGLVLGKALGGGLLPVSAFLADARVMDVFDPGSHGSTFGGNALAAAVAL
                     EALQVIEDENLVARSAALGDYLLQRLAEVATACRPLIRAVRGRGLWVGVDVDPAHATA
                     RELVERLAARGVLSKETHETVIRFAPPLTIGKALIDRAVDIFRETVLDKRKELAISSI
                     APAVASGAEAGDRVKTQDSEPHLLMSPPDFFEVAYAINPWMNPEQWSLDAKRLTRDAL
                     SGWNTLRATYEALGARVVVKPAARGWPDLVFTANCAVVLDGKAILARYLRSERAGEEL
                     HGQRMFEQLKARGEIDAICRTPEGVYFEGAGDAIYDPLRRLMWMGYGQRSCRAAHHSL
                     EQVFGIPTLSLELVDPHYYHLDTAFCLLAGGEILYHPAAFTEEGRGQIRALAGDKLIE
                     APLEDAANLAVNAVCIGRDVVMCSCSAVLRERLEERGYRVHVVPLGSFNRSGGAAYCL
                     TLKLNNVFKGRQRDGQKLAA"
     gene            2194..3249
                     /gene="holA"
                     /locus_tag="VGA59_12375"
     CDS             2194..3249
                     /gene="holA"
                     /locus_tag="VGA59_12375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX9720519.1"
                     /translation="MQLAAAQLSQHLQKGLGPLYTLYGDEPLLIQEAADAIRAAARVQ
                     GYTERTVHTVSGAHFDWSEVLAAGGSLSLFADRQIVEIRIPSGKPGKDGSPALQQLAE
                     NARGNESTLTIVLLPKLDRMAKGGAWFSALESNGVTLQVDPVERPALPQWIAQRLARQ
                     GQRVAAGDEGQRTLQFFADRVEGNLLAAHQEIQKLALLYPEGELGFEQVEGAVLNVAR
                     YDVFKLSEAVLAGQTARVQRMLEGLEAEGVASVLVHYTLAEDIRALKRVKDAMAGGRP
                     LPMALREQRIWGLKERLFERVLPRLTPSSLDSLLHSAHLVDGIVKGLKVPDWPTDGWQ
                     ALHRLAFSLCRECSPQP"
     gene            3346..4626
                     /locus_tag="VGA59_12380"
     CDS             3346..4626
                     /locus_tag="VGA59_12380"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX9720520.1"
                     /translation="MNALNVAEYTQTLGLQAKQASALMARAGAATKIKALRALAGLLR
                     ASVQPLQAENAKDIERAKAAGLAAPMLDRLKLTPRIIETVAQGCEQLAAMPDIIGEII
                     GMKQQPSGIRVGQMRVPIGVFGMIFESRPNVTIEAASLSIKSGNACILRGGSEAIDSN
                     KALARLVQRALVEAGLPADAVQLVQTTDREAVGQLIAMPQYVDVIIPRGGKGLIERIS
                     REAKVPVIKHLDGNCHVYVDDPVDFEMAVRIADNAKTQKYSPCNAIEGLLVARGVAGE
                     FLPKIGAVYAAKGVEMRCDADSRTILASVKGANLKEATEQDWYEEYLAPIVSVKVVAG
                     IDEAIAHINRYSSHHTDAIITHDHMNAQRFLREVDSASVMVNASPRFADGFEYGLGAE
                     IGISTDKFHARGPVGIEGLTSLKWVVLGQGEVRS"
     gene            4713..5729
                     /locus_tag="VGA59_12385"
     CDS             4713..5729
                     /locus_tag="VGA59_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HEX9720521.1"
                     /translation="MRDYWRHCGYALLGRTSDNHLVVTDDFLRALLERPELAPVPESC
                     ERELALHQGLLAQPRAEVDTRELATLADEDARSNYAIWLRFRQRLLAHPTLEAGYTAL
                     FRGEGVDVPPVFVHQLTQILVRHTLGEAATAMQARAAEMMFRPQRISVQDDGQVMAAD
                     DETVERHAISANFGTLGELLKQGGAAMRTAELDVLREDNADVYWERDESHDLVVSLNH
                     GQPALAALCLVLEGWVRHFLGTMVRIAVTRGIDDDNWVWHVGLDAQASAVLNDLYQGR
                     DVGEERMAAMLCLFRLDFDEPAVVRPEIAGRPVYLAMAMDGERRLKLKPQNLLLNLPL
                     ARLS"
     gene            5817..>6240
                     /locus_tag="VGA59_12390"
     CDS             5817..>6240
                     /locus_tag="VGA59_12390"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX9720522.1"
                     /translation="MVPHLITALTGPINELEQRVLDSMPAIERWFRLEWMEHTPPFYS
                     SVDIRNAGFKLAPVDTNLYPGGWNNLTPEMLPLAVQSAMAAIEKICPEAKNLLVIPEN
                     HTRNTFYLANLAQLKRIFHMAGLNVRIGSINPDIKEPTS"
BASE COUNT         1082 a         2036 c         2130 g          992 t
ORIGIN      
        1 cggtgccggt ggtggtggac cacgcgcgcg attgcctggt gtgggacgtg gaaggccgcg
       61 aatacatcga catgatgagt gcgtattcgg ccgtgagcca cgggcacctg catccccgcc
      121 tggtggcggc cgcgcatcgc cagctcgaac gggtggcggt aacttcgcgc gcctaccacg
      181 gcaccacgct cggccccttc cttgaaaagc tgtgccggct ggccggtttc gagcgggcct
      241 tgccgatgaa taccggcgcc gaagcggtgg agacggcgat caagtgcgcc cggcgctggg
      301 gctaccggat caagggcatc cccgacgggc gcgcggaaat cctggtggcg cgtggcaact
      361 tccatggccg caccagcacg atcatcggct tttcgagcga gcccgaatac cgcgccggct
      421 tcgggccgtt cgcgccgggc ttcgtgcatt tcgacttcgg cgacatggcc agcgtccgcg
      481 ccgccaccag cgccaacacc tgcgcggtgc tggtcgagcc gatccagggc gaggccgggg
      541 tgatcgtgcc gccggccggc ttcttcgccg agctgcgcga atggtgcgat cgcaacaacg
      601 tgctgctgat catcgacgag gtccaggccg ggctgggccg caccggccgc tggttcgcct
      661 tcgagcatga aggtatccgc cccgacggcc tggtcctggg caaggcattg ggcggcgggc
      721 tgctgccggt cagcgccttc ctggccgatg cgcgtgtgat ggacgtgttc gacccgggaa
      781 gccatggatc gaccttcggc ggcaacgcgc tggccgccgc ggtggcgctc gaggcattgc
      841 aggtgatcga ggacgaaaac ctggtggcgc gcagcgccgc gctgggcgac tacctcttgc
      901 agcgtctggc ggaagtcgcc acggcatgcc ggccgctgat ccgcgccgtg cgcggccggg
      961 ggctgtgggt gggtgtggac gtcgacccgg cgcatgcgac cgcgcgcgag ctggtcgagc
     1021 gcctggcggc gcgcggcgtg ttgtccaagg aaacgcacga gaccgtgatc cgtttcgcgc
     1081 cgcccctgac catcggcaag gcgctgatcg accgcgcggt ggatattttt cgcgagacag
     1141 tccttgacaa acgcaaagag ctcgctataa gttccatagc gcccgcggtg gcatcgggcg
     1201 cggaggccgg cgaccgagtg aaaacccagg acagcgagcc ccatctcctc atgagcccac
     1261 ccgatttctt cgaggtggcc tacgccatca atccctggat gaaccccgag cagtggtcgc
     1321 tcgacgccaa gcgcctgacg cgcgatgcgc tctcgggctg gaatacgctg cgcgccacct
     1381 acgaggcgct cggcgcccgg gtcgtggtca agccggccgc caggggctgg cccgacctgg
     1441 tcttcaccgc gaactgcgcg gtggtgctgg atggcaaggc catcctggcg cgctacctca
     1501 ggtccgagcg ggccggcgaa gagcttcatg gccagcgcat gttcgagcag ctcaaggcgc
     1561 gcggcgagat cgacgcaatc tgccgaacgc ccgagggcgt gtacttcgag ggtgccggcg
     1621 atgcgatcta tgacccgctg cgccgcctga tgtggatggg ttatggccag cgctcctgcc
     1681 gtgccgccca ccacagcttg gaacaggtgt tcggcatccc cacgctctcg ctggagctgg
     1741 tcgacccgca ttactaccac ctcgacacgg ccttctgcct gctcgcgggc ggggaaatcc
     1801 tgtaccaccc ggccgccttc accgaagagg gtcgcggcca gatacgcgcc ctggccggcg
     1861 acaagctgat cgaggcgccg ctggaggacg cggcgaacct ggcggtgaat gctgtctgca
     1921 tcggtcgcga cgtcgtcatg tgctcgtgct ccgcggtgct gcgcgagcgg ctggaggagc
     1981 gcgggtaccg ggtacatgtg gtgcccctgg gctcgttcaa tcgctccggc ggcgccgcct
     2041 actgcctcac cctgaagctg aacaatgtgt tcaaggggcg gcagcgcgat gggcagaagc
     2101 tggccgcata gggtccgtca ttgcggaccc cggatcgggg tccggggcag gcccgatccg
     2161 caatccatgt tggcatgaat acacttcgtc cttatgcaac ttgccgccgc ccaactcagc
     2221 caacacctgc aaaagggcct cgggccgctc tatacgctgt acggcgacga gccgctgctg
     2281 atccaggagg cggcggacgc gatccgagcg gctgcgcgcg ttcagggcta caccgagcgc
     2341 acggtgcaca ctgtttccgg cgcgcacttc gactggagcg aagtactggc ggccggcggc
     2401 tcgctcagcc tgttcgccga caggcagatc gtcgagatcc ggattccctc gggcaagccg
     2461 ggcaaggatg gcagtccggc cctgcagcag ctggccgaaa acgcgcgggg caacgaaagc
     2521 accctcacca tcgtgctgtt gcccaagctg gaccggatgg ccaaaggcgg tgcctggttc
     2581 tcggcgctcg agagcaatgg cgtgacgctg caggtcgatc cggtggagcg gccggccctg
     2641 ccgcaatgga tcgcgcagcg gctggcgcgc cagggccagc gcgtggccgc cggcgacgaa
     2701 gggcagcgca cgctgcagtt cttcgccgac cgggtcgagg gcaacctgct ggcggcgcac
     2761 caggaaatcc agaaactggc gctgctgtat cccgaaggcg aactcggatt cgagcaggtg
     2821 gaaggcgccg tgctgaacgt ggcgcgctat gacgtcttca aactctccga agccgtcctc
     2881 gcgggccaga ccgcgcgggt gcagcgaatg ctggagggcc tcgaggccga gggcgtggcc
     2941 agcgtcctgg tgcactacac gctggccgag gacatccgcg cgctcaagcg cgtgaaggat
     3001 gcgatggccg gcggccggcc gctgccgatg gcgcttcgcg agcagcgaat ctggggcctc
     3061 aaggagcggt tgttcgagcg cgtgctaccg cgcctgaccc ccagctcatt ggacagcctg
     3121 ctgcattcgg cgcatctggt ggacgggatc gtcaagggcc tgaaggtgcc ggattggccg
     3181 accgacggct ggcaggcgct gcatcggctg gctttttcgc tgtgccgcga gtgctcgccg
     3241 cagccctgag tcgcaacggg cgggtcggat cgcatcgcat ttcgcgggtc gtaccgtcag
     3301 gattcgatca acgcaacctt gccgggcatg agaaaatgcc ttcccatgaa tgcgctgaac
     3361 gtcgccgagt acacgcaaac gctgggcttg caggccaaac aggcctcggc gctgatggcg
     3421 cgcgccggcg cggcaaccaa gatcaaggcg ttgcgtgcac tggccggcct gcttcgcgcc
     3481 agcgtgcagc cgctgcaagc cgagaacgcc aaggacatcg aacgcgcaaa ggcagccggc
     3541 ctggccgcac ccatgctgga ccgcctcaag ctgacgccca ggatcatcga gaccgtggcg
     3601 cagggctgcg aacagcttgc cgccatgccc gacatcatcg gcgaaatcat cgggatgaag
     3661 cagcagccca gcggcatccg cgtgggccag atgcgcgtgc cgatcggcgt gttcggcatg
     3721 attttcgaga gccggcccaa cgtgaccatc gaggccgcca gcctgtcgat caagagcggc
     3781 aacgcctgca tcctgcgcgg cggctcggag gcgatcgatt ccaacaaggc gttggcccgc
     3841 ctggtgcagc gggctttggt ggaggccggc ctgcccgcgg acgcggtgca gctggtgcag
     3901 accaccgacc gcgaggctgt gggccagctg atcgccatgc cgcagtatgt ggatgtgatc
     3961 atcccgcgtg gcggcaaggg cctgatcgag cgcatcagcc gcgaggccaa agtccccgtg
     4021 atcaagcacc tggacggcaa ctgccacgtg tacgtggacg atccggtcga cttcgagatg
     4081 gcggtgagga ttgccgacaa tgccaagacg cagaagtaca gtccctgcaa tgcgatcgag
     4141 ggcctgctgg tggcgcgcgg ggtagccggg gagttcctgc cgaagatcgg tgcggtctac
     4201 gccgccaagg gtgtggaaat gcggtgcgac gccgactcgc ggaccatcct cgcgagcgtg
     4261 aaaggcgcca acctgaagga agccaccgag caggactggt acgaggaata cctcgctccc
     4321 atcgtcagcg tcaaggtggt ggcgggcatc gacgaggcca tagcgcacat caaccgctat
     4381 tccagccacc acaccgacgc catcatcacg cacgatcaca tgaatgcgca gcgcttcctg
     4441 cgcgaggttg attcggccag cgtgatggtc aacgccagtc cgcgcttcgc cgacggcttc
     4501 gaatacggat tgggggcgga gatcggcatc agcaccgaca agttccacgc ccgcgggccg
     4561 gtcggcatcg aggggctgac ctcgctcaag tgggtggtgc tgggacaggg cgaagtgcgt
     4621 agttgatcag ttgaggacag agctgaagat ctgtcccgca agcttagtca gttgaggaca
     4681 gagctgaaga tctgtcccgc aaggtatcag gaatgcgcga ctactggcgc cactgcggct
     4741 acgccctgct gggcaggact tcggataacc acctggtggt taccgacgac ttcctgcgcg
     4801 ccctgctgga gcggcccgaa ctcgcaccgg tgcccgaatc ctgcgagcgc gaactcgcgc
     4861 tgcaccaggg cctgctcgct cagccgcgcg ccgaagttga cacgcgggag ctcgcgactc
     4921 tggccgacga ggacgcgcga tccaactacg ccatctggtt gcggtttcgc cagcgcctgc
     4981 tcgcgcaccc cacactggaa gccggctaca cggcattgtt ccggggcgag ggtgtcgatg
     5041 tgccgcccgt gttcgtgcac cagctcacgc agatcctcgt gcgccacacg ctgggcgagg
     5101 ccgccacggc gatgcaggcg cgcgcggccg agatgatgtt ccggccgcag cgtatctcgg
     5161 tgcaggacga cgggcaggtg atggccgcag acgatgaaac ggtggagcgg cacgccattt
     5221 cggccaactt cggcaccctc ggcgagctgc tgaagcaggg cggagcggca atgcgaacgg
     5281 cggaattgga cgtgctgcgc gaggataacg ccgacgtcta ctgggagcgc gacgagagcc
     5341 acgacctggt ggtcagcctc aaccatggcc agccggcgct ggcagcgctg tgcctcgtgc
     5401 tcgagggctg ggtccggcat ttcctcggaa caatggtgcg catcgctgtg acacgcggca
     5461 tcgacgacga caattgggtc tggcatgtgg ggctggatgc ccaggccagc gctgtcctga
     5521 acgacctgta ccagggccgc gacgtgggcg aggaacgcat ggccgcgatg ctctgcctgt
     5581 tccggctcga cttcgacgag ccggccgtgg tgcggcccga aatcgccggc cggccggtgt
     5641 acctggcgat ggcgatggat ggcgaacgcc gcctcaagct caagccgcag aacctgctgc
     5701 tcaacttgcc gttggcgcgg ctgtcctaaa agcccggcga cacttgcaac gggcatagtc
     5761 gccctcaaat ccctacaatt cctgtcagtt atcccccccg aaggcaagag gaccgcatgg
     5821 ttccgcatct gatcaccgcc ctgaccgggc ccatcaacga gctcgagcag cgcgtcctcg
     5881 attccatgcc cgccatcgag cgctggttcc gcctggagtg gatggagcac acgccgccgt
     5941 tctacagctc ggtggacatc cgcaatgccg gcttcaagct ggccccggtc gataccaacc
     6001 tctaccccgg aggctggaac aatctcacgc ccgagatgct gccgctggcc gtgcaatcgg
     6061 ccatggccgc gatcgaaaag atctgccccg aggcaaagaa cctgctggtg atccccgaga
     6121 accacacgcg caacactttc tacctggcca acctggccca gctcaagcgc atcttccaca
     6181 tggccgggct gaatgtgcgg atcggctcga tcaatccgga tatcaaggag ccgaccagcg
//
LOCUS       DASWIY010000348         3656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3543167, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000348 DASWIY010000000
VERSION     DASWIY010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3543167"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VGA59_12395"
     CDS             <1..807
                     /locus_tag="VGA59_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9720523.1"
                     /translation="MIGAIAMSEPAAGSDLQGIKGTAIKQADGSYRLSGSKTFITNGW
                     HADLVIVVAKTNPQAGAKGTSLLLVERGMKGFEYGKRLKKLGLKAQDTAELFFDNVRV
                     PADNLLGGEAFENKGFICLMEQLPWERLQIAVTAVASAQAAIDGTVQYTKDRKVFGQP
                     VAAYQNTRYVLAELQTQVQIARVFVDKCLELLCLDKLDTATASMAKYWTTDLQCKVMD
                     ECVQLHGGYGYMWEYPITRAYADARVQRIYGGTNEIMKEVITRAMGLGGQ"
     gene            complement(844..1188)
                     /locus_tag="VGA59_12400"
     CDS             complement(844..1188)
                     /locus_tag="VGA59_12400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX9720524.1"
                     /translation="MTPDRGDILHLAFDPASGREMKGNHFCMVVSPRAFNSRFKLAMV
                     CPISGGAAEAARSAGFLVSLMGQGLRTDGQVHAHQIKSLDWASRKASLVERAPGVVVQ
                     EVLTCLQSVLAD"
     gene            complement(1185..1427)
                     /locus_tag="VGA59_12405"
     CDS             complement(1185..1427)
                     /locus_tag="VGA59_12405"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9720525.1"
                     /translation="MLHASLRRSGGSYIMTVPLSYVEQNQLEAGSRLAIEIVGDELKV
                     RPQRQRKTLAELLKATPRGLHRAQGWDELGAAGAEL"
     gene            1465..1875
                     /locus_tag="VGA59_12410"
     CDS             1465..1875
                     /locus_tag="VGA59_12410"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX9720526.1"
                     /translation="MLEVIEMTIAYWCVLVALLAPYILSVAARSQVSRADYVQDPRAY
                     SESLTGWRRRAHLAHLNAFEAVPAIIAGVMVAEFSHAPRAYVDALALAFVAFRAMHAA
                     CYIADRPMLRSHAWRLGIVCIIGLFVVAAVQGRS"
     gene            complement(1899..3317)
                     /locus_tag="VGA59_12415"
     CDS             complement(1899..3317)
                     /locus_tag="VGA59_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9720527.1"
                     /translation="MLQLTTLDGGTADISQEDLQAFKTLFKGAVLTPDDPAYEETRKI
                     WNAMIDRRPGLIARCTGTVDVVQAVRLARKHRLLVSVRGGGHNIAGLAVCEGGLMIDL
                     SNMRGVWVDPLNGTARAQAGCTLADVDRETQLHGQAAVLGFISTTGIAGLTVGGGFGY
                     LTRQHGCTCDSLVSVEVVTADAKVLRASIEENEELFWALRGGGGNFGIVTSFEYRIYP
                     VGPEILGGAIAWRGEDTKQVLDTYREFSATAPRELTSVAALRIAPPAPWLPKEIHGKP
                     IAAIFICHTGKIEDGEKLVAPLRKLGQPVADIVTRRPYTQMQSLLDATQPKGRRYYWK
                     SHYLGNLSQEFDDVAVLHAARITSPFSAILLFQIAGALNQLPDEHSPVGNRDAAYVLN
                     IAGSWERPEDDEVNIRWARECFEATRPFSTGGTYVNFLTEEEGADRIEAAYGRANLAR
                     LAELKRKYDPDDLFRHTKRVSV"
BASE COUNT          639 a         1184 c         1175 g          658 t
ORIGIN      
        1 atgatcggcg ccatcgccat gagcgagccg gcggccggca gcgatctgca aggcatcaag
       61 ggcaccgcca tcaagcaggc cgacggcagc taccggctca gcggcagcaa gaccttcatc
      121 acgaatggct ggcatgccga cctggtgatc gtggttgcca agacgaatcc gcaagccggc
      181 gccaagggca ccagcctgct gctggtcgag cgcggcatga agggattcga gtacggcaag
      241 cggctgaaga agctgggctt gaaggcgcag gacaccgccg agctgttctt cgacaacgtc
      301 cgagtcccgg ccgacaacct gctgggcggc gaggcgttcg agaacaaggg cttcatctgc
      361 ctcatggagc agctgccatg ggagcgcctg cagatcgccg tgacggcggt ggcctcggcc
      421 caggccgcga tcgacggcac ggtgcagtac acgaaggacc gcaaggtgtt cggccagccg
      481 gtggccgcgt accagaacac ccgctatgtc ctggccgagc tgcagacgca agtgcagatc
      541 gcccgcgtct tcgtggacaa atgcctggag ctgctctgcc tggacaagct cgacaccgcg
      601 accgccagca tggccaagta ctggaccacg gacctgcaat gcaaggtcat ggacgagtgc
      661 gtgcagttgc acggcggcta tggctacatg tgggaatacc ccatcacccg cgcctatgcc
      721 gacgcgcgcg tgcagcgcat ctacggcggc accaacgaga tcatgaaaga ggtcatcacg
      781 agggcgatgg ggttgggcgg acaatgagta ctcgttggct agagtcctca gtgtgaacag
      841 gccctaatca gcgagcacgc tttgcagaca tgtcagcacc tcttgcacga ccacgccggg
      901 tgcgcgttcc accaggctgg ccttgcgtga ggcccagtcc agtgacttga tctgatgcgc
      961 gtgaacctgc ccgtcggtgc gcaggccttg acccatcagc gacaccaaaa agccggcact
     1021 gcgggctgcc tcggcggcac cgccggaaat ggggcacacc atcgccagct tgaagcggga
     1081 gttgaaggcc cggggcgaaa caaccatgca gaagtggttg cctttcatct cccgaccgct
     1141 ggccgggtca aaggccagat gcaggatgtc gccccggtcg ggcgtcacag ctctgccccg
     1201 gctgccccca gttcgtccca accctgcgca cgatgcaggc cgcgaggtgt tgctttcaac
     1261 aactcggcca gggtcttgcg ttggcgctgg ggcctgacct tgagttcatc cccgacgatt
     1321 tcgatcgcca ggcgcgatcc cgcttccagt tggttttgct ccacatagga aaggggcacc
     1381 gtcatgatgt aagagccgcc cgagcggcgc aaggaagcgt gcagcattgg cgttctccaa
     1441 agttttacaa gagtataacc ttggatgctg gaggtgatcg aaatgacaat cgcgtactgg
     1501 tgtgtcctgg tggctcttct cgcgccgtac atcctgtccg tcgccgcacg ctcgcaagtc
     1561 tctcgtgcgg attacgtcca ggacccccgg gcctacagtg aaagcctcac cggctggcgc
     1621 cggcgtgcac atcttgccca cctcaatgca ttcgaggcgg tgcccgccat cattgccggc
     1681 gtcatggtgg cggagttctc gcacgcaccg cgtgcctacg tcgacgcact ggctctcgcg
     1741 tttgtcgcct ttcgggccat gcatgccgcg tgctacatcg cggacaggcc catgttgcgc
     1801 agccatgcat ggcggctggg catcgtctgc atcatcggcc tctttgttgt cgcggccgtt
     1861 caagggcgtt cttgatttgc ccgcctggcg gccatgattc agaccgacac ccgcttcgtg
     1921 tggcggaaca ggtcgtcggg gtcatacttg cgcttgagct cggcgagccg ggcgaggttg
     1981 gcacggccat acgcggcttc gatgcggtcg gcgccttcct cctcggtcag gaagttcacg
     2041 taggtgccgc cggtcgagaa cgggcgcgtc gcctcgaagc attcgcgtgc ccaacggatg
     2101 ttgacttcgt cgtcttccgg cctttcccag gaacccgcga tgttcagcac ataagcggcg
     2161 tcgcggttgc ccacgggcga gtgctcgtcg ggcaactgat tcaaagcgcc cgcgatctgg
     2221 aacagcagga tcgccgagaa cggcgacgtg atgcgcgccg cgtgcaacac ggccacgtcg
     2281 tcaaattcct gggacaggtt gccaaggtaa tgcgatttcc agtagtagcg ccttcccttc
     2341 ggctgcgtcg cgtccagcag gctctgcatc tgcgtgtaag ggcgccttgt cacgatgtcc
     2401 gccaccggct ggccgagctt gcgcaaaggc gcgacgagct tctcgccgtc ttcgatcttc
     2461 ccggtgtggc agataaagat cgcggcgatc ggcttgccgt gaatttcctt gggcagccag
     2521 ggcgcgggag gagcgatgcg caaggctgcg acgctggtca gctcgcgcgg cgccgtggcg
     2581 ctgaactcac ggtaggtgtc cagcacctgc ttcgtatctt cgccgcgcca tgcgatcgcc
     2641 ccacccagaa tctcggggcc gaccgggtat atccggtatt cgaatgaggt gacgatgccg
     2701 aagttgccgc cgccgccgcg cagcgcccag aacaactcct cgttttcttc gatcgaagcg
     2761 cgcagtacct tggcgtccgc cgtcaccacc tccaccgaga cgaggctgtc gcaggtgcag
     2821 ccatgctggc gggtgagata gccgaagccg ccgccgaccg tcagccccgc gatgccggtt
     2881 gtcgagatga aaccgagcac cgcggcctgg ccgtgcaact gcgtctcgcg gtcgacgtcc
     2941 gccaaggtgc agccggcctg ggcacgcgcc gtgccgttca ggggatcgac ccacacgcct
     3001 cgcatgttcg acaggtcgat catcaagccg ccctcgcata ccgcgagccc ggcgatgttg
     3061 tggccgccgc cgcgcaccga caccagcagc cggtgcttgc gcgcgagccg cactgcctgc
     3121 accacatcga ccgtgcccgt gcagcgggcg atcaggccgg gacgccggtc gatcatcgcg
     3181 ttccagatct tgcgggtttc ctcgtaagcg ggatcgtccg gcgtgagcac ggcgcccttg
     3241 aacagggtct tgaaagcttg caggtcttcc tgcgaaatgt cggcggtgcc gccgtcaagg
     3301 gttgtcagct ggagcatgat tcgtctccta tttttggggc gatcaggaga cgcagcatgc
     3361 cagctgtcgg gggcctttgg ctagggcctg ttcacactgt tgcagagccc gatgagccgg
     3421 cctttccgcc cacagcgcag ttagcgtgcc aggataagca ggacggctga gcgcggccgg
     3481 cgcagcggcg gccggcctga cgggcacgcg ttcacagtcg agccgccacg atggcgtacc
     3541 cttgagggag ttgcctatcg gagcacctca tgctgcgcgc cttgaaagac ctgttcgaca
     3601 ccgtctccgc gcccgtcgac gcgcaaccgg cgtcgtccga acacctgctg cagctc
//
LOCUS       DASWIY010000349        22213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2824143, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000349 DASWIY010000000
VERSION     DASWIY010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 22213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22213
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2824143"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..962)
                     /gene="msrA"
                     /locus_tag="VGA59_12420"
     CDS             complement(327..962)
                     /gene="msrA"
                     /locus_tag="VGA59_12420"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX9720528.1"
                     /translation="MAASAAAEKAVKLPAPAFDTPAAVQGPQTAVFAGGCFWGVQAVF
                     QHTRGVLNAVSGYAGGTRESANYQAVTTGATGHAEAVQVTYDPKQVSYGKLLQIYFSV
                     AHDPTQLNRQGPDVGPQYRSALFFAGKDQKDVAGRYIAQLEAARAFPRKIVTQLAPLQ
                     AFYPAEAYHQDYATRHPDSPYIVAFDRPKIANLKSIMPEVFREAPVLVSHN"
     gene            complement(1055..1561)
                     /gene="msrB"
                     /locus_tag="VGA59_12425"
     CDS             complement(1055..1561)
                     /gene="msrB"
                     /locus_tag="VGA59_12425"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017425457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX9720529.1"
                     /translation="MSNRRNTLLGLGILSGLAAGVRGLFASGPAEAAQPTRSYELTRT
                     DAEWRKLLSNEQYQVLRRAGTERPYSSPLDKEKRAGVFNCAGCGLPLFSSSTKFNSGT
                     GWPSFWQPLDKAVDEKVDSSLGMRRVEVLCRRCGSHLGHVFDDGPKPTGLRYCMNGVA
                     LTFTSQKT"
     gene            complement(1973..2255)
                     /locus_tag="VGA59_12430"
                     /pseudo
     CDS             complement(1973..2255)
                     /locus_tag="VGA59_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            2226..3110
                     /locus_tag="VGA59_12435"
     CDS             2226..3110
                     /locus_tag="VGA59_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX9720530.1"
                     /translation="MSTTLTHPLSLGSSGTAVRALQTQLNASQLALGPKLSVDGSFGK
                     VTHIAVIAFQRRKGLKADGVVGAKTARALAWNYQPVNEKPYIVRYDKPPLPAATPPLA
                     VVAEAIRAGMDPLTEKLADDLWVAFNDPNNDPNYRTLMGKAHAHDPSLSQKQNRQIIL
                     RIKDLIFHYKKFSEVLSQLGDLSASDPEIVPSRLRGAFKEFVNQIISACNAMDFYYGI
                     TEHCRKRLNRLPYESIVGKVESVLKGDRTVTFAVAEIQMLFQTMAYAAVFDRSKVVDR
                     PTLDWLERFEYHLKPNFN"
     gene            3130..3375
                     /locus_tag="VGA59_12440"
                     /pseudo
     CDS             3130..3375
                     /locus_tag="VGA59_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
     gene            3706..4344
                     /locus_tag="VGA59_12445"
     CDS             3706..4344
                     /locus_tag="VGA59_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX9720531.1"
                     /translation="MPQLIASVEGVQIKHVYLQQDRTTLGRHPDNDIVLDNMVVSSHH
                     CVFELKGLADVFIEDLRSTNGTYINGKMVKRQRLHDGEVIAIGNFRIQYLAESERPSG
                     FSSSGFSHTTAMELDAQGVPVAHAFFRVLTGSSAGLEVPVVKAVSTFGKPGVSVVAVS
                     HRRDGFYVAHLDGDAPPTLNGDRIGSQAVMLSNHDVVELAGTRMLFQLKEQP"
     gene            4398..5003
                     /locus_tag="VGA59_12450"
     CDS             4398..5003
                     /locus_tag="VGA59_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720532.1"
                     /translation="MLKKFFGGGRGAGESQPPHSSQFHESETTAQHHGSRNAPRRELV
                     QLVLRDTMRKHGIPSDWIDCRILSVVTRRHMSGMHVQFIVDKGQSRLLAYVQPFQESF
                     WEEIEKFEPHARDWLFSLAWQFDGPGTRGRSMPGPGGWYGDEARAGGDTQSPDADADE
                     LASDLQALYAIRDAALSPPAERTAPQGGPEPSSRPGGPPDS"
     gene            5075..5323
                     /locus_tag="VGA59_12455"
     CDS             5075..5323
                     /locus_tag="VGA59_12455"
                     /inference="COORDINATES: protein motif:HMM:NF019016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 family protein"
                     /protein_id="HEX9720533.1"
                     /translation="MSISDPVAKEDGVEFTATRGGVEQKFLAHREALQDLDYSVFETA
                     EALLKAFHRHQGQIAAIAGRAMDQGVGAGTTVVLQSLL"
     gene            5437..5874
                     /gene="trxC"
                     /locus_tag="VGA59_12460"
     CDS             5437..5874
                     /gene="trxC"
                     /locus_tag="VGA59_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEX9720534.1"
                     /translation="MSEALHVVCPHCHTTNRVGQGDLAKAPDCGNCHKPLFTARSTPL
                     DEAAFERHIARNDIPVLVDFWAPWCGPCRQMAPAFEQAAAQLEPKVRVAKVNTEEAQS
                     LGARHRIRSIPTLALFMKGREVARQAGAMGAADIVRWTQAQLR"
     gene            complement(5899..6429)
                     /gene="thpR"
                     /locus_tag="VGA59_12465"
     CDS             complement(5899..6429)
                     /gene="thpR"
                     /locus_tag="VGA59_12465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX9720535.1"
                     /translation="MQAVAPDAGKPLRLFVALWPKEEVRLAIASWQQAWDWPPRAARV
                     KPERLHITLHFLGDVPADRLLHLTRALRVPFESFTLELDQGEVFPNGVAVLRSAANPP
                     ALLRLHAALRHRLVDLDLPVETRPYLAHVTLARRAKGARPPPQGPALRWHIDSGYVLV
                     RSLPGGAGYQVLERFN"
     gene            6487..6891
                     /locus_tag="VGA59_12470"
     CDS             6487..6891
                     /locus_tag="VGA59_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720536.1"
                     /translation="MTKADIPDEVRRFILTSVPSVPYLEAIILLRAEPKGPWDAQRLS
                     RRLYLPEPRTAELLQALAAAGIAEPAQQEASQRESEAFRFKPATPELDAMLDQLTRVY
                     AGDLMGVTDLIHSRVDKRAQQFADAFRWRKDS"
     gene            6893..7150
                     /locus_tag="VGA59_12475"
     CDS             6893..7150
                     /locus_tag="VGA59_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEX9720537.1"
                     /translation="MAAFIYSLCALTCLAAFGLLLRSWRATRHRLLFWSALCFAFLSL
                     NNILLVVDNLVLPQVDLSTPRLLSALLGVLLLLFGLVWEEE"
     gene            7157..7414
                     /locus_tag="VGA59_12480"
     CDS             7157..7414
                     /locus_tag="VGA59_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEX9720538.1"
                     /translation="MNQMLMGAIAVASLLASLFFLRFWRHTRDRFFLYFALSFAIEGF
                     NRLVMGLLDATREDNPVFYSVRIVAYGLIVAAIWQKNRPRP"
     gene            complement(7424..8179)
                     /locus_tag="VGA59_12485"
     CDS             complement(7424..8179)
                     /locus_tag="VGA59_12485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9720539.1"
                     /translation="MTTIAGPAGQLHVDDGGAGALPVLLVHSYAGSSSHWKAQLAHLR
                     RRRRALAMDLRGHGRSDAPASGDHSVPALAEDIAAVADALELSRFVLVGHSMGGAAAS
                     AYAGRHPRRVAGLVLAGSPGKSSASEAQKIMDALRADYDNVMAGYWSSLLQGAEPPVR
                     ELLDAGMRSVGREPSLAMIGAIFDFDPLPDLGRYPGPKLIIDTPHGDSPTALHRQLPD
                     VSRKVIIGTSHWPHMDKPLEFNRLLDEFLAWLA"
     gene            complement(8192..8818)
                     /locus_tag="VGA59_12490"
     CDS             complement(8192..8818)
                     /locus_tag="VGA59_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9720540.1"
                     /translation="MQQAAPRHFSMLRGFHVADFFTLGNAACGVGAVFFAMLFVAGES
                     PLHFYAAAALAPAAFIFDVLDGRIARARHEHSPLGRELDSLSDVISFGVAPAALGFAA
                     GLQGGWDVAALIFFVCCGVSRLARYNVTAEELSAGSDKVSYFEGTPIPTSVVLTGLLA
                     FAAAQGRLGDELYGGAYTIGPWVLHPLALLFVLSGSLMISKTLHIPKL"
     gene            complement(8988..9275)
                     /locus_tag="VGA59_12495"
     CDS             complement(8988..9275)
                     /locus_tag="VGA59_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX9720541.1"
                     /translation="MKPNQTAARIALAATLLAAGTAFAAGPTNAEYYPFQEIMSMKML
                     DKNKDGMVSKKEFIDMMNLTWDMNARKMGAKGEAMNADQFKEILMYLKAGG"
     gene            complement(9358..9627)
                     /locus_tag="VGA59_12500"
     CDS             complement(9358..9627)
                     /locus_tag="VGA59_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720542.1"
                     /translation="MKPNQVAAALTFAATLLAAGTAIAAGPTNAEFYPFQETTSSVKV
                     IDSSTAMKSTTKSTNGDTKTMGAQGHASSDAQSSSEILMYLRAGG"
     gene            9920..10345
                     /locus_tag="VGA59_12505"
     CDS             9920..10345
                     /locus_tag="VGA59_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9720543.1"
                     /translation="MDIEQFMQGYKRAWEGRDPALFCALFAQDGVYRNTPFAVQRGHA
                     QLAQYWERVKLQEDVKVLFDVLASAPASGIAHWRVTYQVASEELFRIWAASTGTNLVA
                     RKPGDPLPRMVLDGVLHARFSGELCSECRIWWHSMPVPA"
     gene            10386..10691
                     /locus_tag="VGA59_12510"
     CDS             10386..10691
                     /locus_tag="VGA59_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720544.1"
                     /translation="MTSTSKILAALLGGYVLLVVPAYVGPAFLEEFSGFFVLVPLLSI
                     YLFHKVGVPGLLEHGGACGWGWCAPTVFGWIFAALFWTGLAWLAAAGLARLISRFSR"
     gene            10688..11164
                     /locus_tag="VGA59_12515"
     CDS             10688..11164
                     /locus_tag="VGA59_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide prediction"
                     /protein_id="HEX9720545.1"
                     /translation="MRLAALTGARAALRLLAYAWAAPYSLLGLTVGLLALLLGARARL
                     REGAMEFGGGRLGVRLSRLPAPFCFSAITVGHVILGIDEATLAAARAHEHVHVRQYER
                     WGPLFVPAYLLSSLAQLVRGRRPYLDNRFEREAYSVAGCGPGAAQGRSLPKPGEQR"
     gene            11231..11752
                     /locus_tag="VGA59_12520"
     CDS             11231..11752
                     /locus_tag="VGA59_12520"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9720546.1"
                     /translation="MKADDLRALQSPLKERYRNEPAAALITLSAQGRLGEGVTCRIET
                     GKALVAAGLHPATGGTGMSACSGDMLLEALVACAGVTMNAVATSLGIELRDAAVHAEG
                     DLDFRGTLGVSRETPVGFQAIRLRFTLDTDASQEQLSTLVRLTEKYCVVYQTLAHSPA
                     LGVTTEVGRVAPV"
     gene            11775..12428
                     /locus_tag="VGA59_12525"
     CDS             11775..12428
                     /locus_tag="VGA59_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9720547.1"
                     /translation="MDSAPTSPVRHAGGFYENRIFPWLNDRLARDPELERTRGEALAP
                     ARGRVIEIGFGSGLNLRHYPAAVQSIVAVEPNAGMHERAAGRIKASRIPVEVVMGTAE
                     SMPLPAGAFDTAVSVLTMCSVADPVRVLSELRRVLRDDGRLLVLEHGLADEPGVARWQ
                     NRLNGIQRTVGCGCNLNRPMARLVEGHGFRFEAVRRFYAPKIPRTHGWLTVGTAVKA"
     gene            12525..12872
                     /locus_tag="VGA59_12530"
     CDS             12525..12872
                     /locus_tag="VGA59_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9720548.1"
                     /translation="MQYILLIYGNEAAMQSATESQVAQMVAAYGAYSEALKKAGVIVG
                     GERLKPVSSATTVRVTGGKTEVLNGPYAETKEQLGGYYLIDVPDLDAALSWAARCPGA
                     NAGTIEVRPIWAM"
     gene            12877..14121
                     /locus_tag="VGA59_12535"
     CDS             12877..14121
                     /locus_tag="VGA59_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX9720549.1"
                     /translation="MTHAMVAREAAEGAARRSYGKLVAFLAARTRDVAGAEDALAEAL
                     ATALATWPARGVPDNPEAWLLTVARRKMIDAMRERQQGEGAAEHFALLAELAEGEEND
                     ATIPDDRLALMFACAHPAIEAGIRAPLMLQTILGFDAAAIASAFLVSPATMGQRLVRA
                     KSRIRQAGIPFRVPGRAELRERLVPVLDAIYAAYAEGWADAAGTDARRRNLAEEAIWL
                     GGLLASMLPQEPEALGLLSLMLHAQARRRARRDADGAYVPLDEQDVALWDERLIVEAE
                     ALLRRASALEGIGRFQLEAAVQSAHVVRRRDGRADWAAIERLYEALETLTASPVVSIN
                     RAIAIAQTQGPQAGLQLLGSLAADVRLADYQPYWAARAGLLARTGDIPQALSSYERAI
                     GLERDPAVRQFLQRQREKLTGR"
     gene            14139..14465
                     /locus_tag="VGA59_12540"
     CDS             14139..14465
                     /locus_tag="VGA59_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720550.1"
                     /translation="MARRHHYHVYVVELSDQVWNVGRFRRANPHYQLGKPFVYVGMTG
                     LDPDVRLDKHKAGIQANSFVRDYGLRLLPGLYAVYNPMPYDAAREMEVELGIALREAG
                     YGVWQA"
     gene            complement(14496..15617)
                     /locus_tag="VGA59_12545"
     CDS             complement(14496..15617)
                     /locus_tag="VGA59_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9720551.1"
                     /translation="MNRVARMALAGAALAMAQLGQAAQIVVGQVGPMSGLEANQGRAY
                     AAGMQLLFTGVNKAGGVNGHTFTLVRKDDGGRPEDTVSATRQLLAEEKPMVLAGFFGS
                     RNITDLVSSGVLAKDRIALVGYRSAEIRPETPLLYSVRAGLRDEINKLTQHLATIGIQ
                     RLGLLYEEGPGAPALLAAADQAARKGNATIISKASYATGTARVKPAIATFMATPPQAI
                     IMVSSGAAAAGFIEEYRSAGGTAQLFAHSGADIEQLSKRLAEEQMQGVAIAQVTPSPY
                     KISSRLSKEFTDAVAGTEKLEVPVSYAMMEGYIAAKVIVEAVRRQGARPSREGMVSAL
                     DRMDNYNMGGYAVGFKPGMRSGSKFVELSIISTAGKIRQ"
     gene            15751..17025
                     /locus_tag="VGA59_12550"
     CDS             15751..17025
                     /locus_tag="VGA59_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9720552.1"
                     /translation="MTTLAATRKDIESPYAWMRLAVSLLLMTIGGSGMYSVTVVLPAI
                     QAEFAVARADASLPYTLTMIGFGLGGILMGRLADRFGVLVPVGTGALGLGAGFVLAGL
                     APNLWLFCAAQGLLIGLLGTSATFAPLVADTSQWFDRRRGIALAICMSGNYTAGAAWP
                     PVMQHFIDAVGWRQTYIGMGVFCVLTMLPLALVLRRRPPVHAHDATADTVTSRPATQT
                     ASDRPMGLRPGTVQALLCVAGVSCCVAMSMPQVHIVAYCADLGYGAARGAEMLALMLG
                     LGVVSRLLSGWISDHIGGLRTLLLGSILQGVALLLFLPFDGLVSLYVVAGLFGLFQGG
                     IVPAYALIVREHFSPREAGARVGMVLMATLFGMALGGWMSGAVFDLTGSYRAAFINGI
                     AWNLLNVAIVVFLLYRVRAVARGRRQLSGAPS"
     gene            17041..18630
                     /locus_tag="VGA59_12555"
     CDS             17041..18630
                     /locus_tag="VGA59_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX9720553.1"
                     /translation="MAQHRPIDRRRLLQLAAAGATSLWLPQAARGQPRLTGNPFALGI
                     ASGSPTHDSVVLWTRLVAPGFFETLGEQAIQVRWEVAHDEQFSRVVRRGQSPALFELA
                     HSVHVEVAGLEPDRWYFYRFIAGDAASPTGRTRTFAAPDAAVQKLRLAYASCQRWEHG
                     YYSAYRHMRDENLDAVLFLGDYIYEYPIAANPVRVPTGGWVLTLEDYRRRYALHKSDA
                     DLQAMHMACPWLHIWDDHEVHNDYAGLKPGDSGRLAPLPMDGFVARRAAAYQAFYEHM
                     PLRASVLTRAVAGLASGAEMRIYSRQRVGRLATILMLDARQYKDPQVCMPPGTFGSAS
                     MDPAACPDWNDPKRSMLGAVQEQWLEAALDGSTGWTVIGQQTLFGRRDAKPGSDEIFW
                     NDGWDGYGAARTRLTNALQLHRVANPVILGGDVHQNWVGNVKADYARADSATVGVEFC
                     GTSISSRSAANPRIPERLAENPHFVFADAERRGYGVAEFTPARLTTTLRVVDDVRRRD
                     SGVQTLASFVVEAGRPGLEKS"
     gene            18627..20324
                     /locus_tag="VGA59_12560"
     CDS             18627..20324
                     /locus_tag="VGA59_12560"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX9720554.1"
                     /translation="MRRRWWWCLSCLLALIPAAAQSPPGELTIRGDDSYVLSHAFVYL
                     EDKGGQLTLDDVLKPEASAAFRPLPLTGPGANFGMTSSAIWLRITLKALPTAAPDWLL
                     ELAHPPLDRVELFSANASGSQRRLGGDLQPFGSRAVPHRNHVLPVTLAPGAQTVLHLR
                     LKSEGTVVAPVTLWRPAALWRHDQAAYSVLSLYFGLLIGLLLYNLLLFVSVRDVGYLS
                     YVAFVACMGIAQAALTGLGAQFLWPQWTWWNSVSPLAGIAAAAGFAVLFARNFLSSPI
                     RLRRMDRLMLAQLAGWSLALLAALALPYVVSLWMVTILGVLGVATMLVVGVISVRREF
                     AGARYFFTAWAVLLLGVLTLALHSAGIVPSNAITSNALLIGSALEMVLLSFALADRIN
                     VARRFKEQAQARIAAERAMVEALSQSHGQLRMMLDERELILESSIVGIAFLTPEGRFR
                     WANKAMIEIFGTSFRANTSMEPFYLSREQYLQVGGEVSATVGRGEVYETELRMRRADG
                     TPIWVSLSGKAVSRRDLGQGTVWVVMDITRRKELEEQLERSSSDQARIREALEQQKA"
     gene            20543..21913
                     /locus_tag="VGA59_12565"
     CDS             20543..21913
                     /locus_tag="VGA59_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX9720555.1"
                     /translation="MSLQTKYLLDENQMPKFWYNIQADLPTAAPAVLHPGTLQPVGPN
                     DLAPLFPMELIMQEVSTQREIEIPEPVRDVYRAWRPAPLYRAHRLEKALGTPAKIFYK
                     YEGVSPAGSHKPNTAVAQAFYNQQAGVKRLVTETGAGQWGTALSYAGALFGLEVDVYQ
                     VRVSYDQKPYRRAVMETYGARCVASPSNETVYGRSVLAKRPDHPGSLGIAISEAVEMA
                     VQREDTKYALGSVLNHVLLHQTIIGLEAMEQMQMADAWPDVVVGCTGGGSNFAGIAFP
                     FIGDGLRGGQKPRIVAVEPAACPSLTRGKYAYDFGDTGHMTPLSKMHTLGSAFTPPGF
                     HAGGLRYHGMAPLVSHLKELGMLEAVAYNQVECFEAGVLFARNEGIVPAPEANHAVKG
                     AIEEALRCKREGKAETILFNLCGHGHFDMLAYSKYFAGELSDEKYDENELAMALAGLP
                     SVPEPA"
BASE COUNT         3670 a         7123 c         7464 g         3956 t
ORIGIN      
        1 gaatcttgat catcggcggg ctccttgggg tcgggagtga ctgttcgggt cggcttttcg
       61 agcagtcact cccggcccac aagcagtctt tcgcttcgtc gctccgatcc ggtcactggg
      121 cggcctgctg gagtcaactg cagatgtgtc atccttgcgc cggcgttgtc atatcgccgc
      181 gattctgatg gaccatgtat atcaccgacg taaatcactt tcttgacgct tcccgcagcg
      241 cccatcccct cgcaaaatgc atccatcgtc gtcccagtcg ccacaccgga cggctcacgt
      301 gagatccgag ttcagtcggc ctctcatcag ttgtgcgaca ccagcaccgg cgcctcgcgg
      361 aacacctccg gcatgatgct cttcaggttg gcgatcttgg gccggtcgaa ggccacgata
      421 tagggcgaat cggggtgccg ggtcgcgtag tcctggtgat aggcttcggc cggatagaac
      481 gcttgcagcg gcgccagctg cgtcacgatc ttgcgcggga acgcccgcgc cgcctccagt
      541 tgggcgatgt aacgcccggc cacgtctttc tggtccttgc cggcgaaaaa aagcgccgag
      601 cggtattgcg ggccgacatc cgggccctgc cggttgagct gcgttggatc atgggcgaca
      661 gagaagtaga tctgcagcag cttgccgtag gagacctgtt tcgggtcgta ggtgacctgc
      721 actgcctccg catggccggt cgccccggtc gtcacggcct ggtaattggc gctctcccgg
      781 gtgcccccgg cataacccga gacggcattg agcacgccgc gtgtgtgctg gaacacggcc
      841 tgcacccccc agaagcaccc gccggcgaac accgccgtct gcggtccctg gacggccgcg
      901 ggagtgtcga acgccggcgc cggcagcttg accgctttct cggccgcggc gctggcggcc
      961 atccaggcac ccacggcgat cgcgacggcg atcaccggta tcttgtccgc gagaaaacca
     1021 gcggcgttgg ccacgaagcc ggcgggtcgg attttcatgt cttttgggaa gtgaaggtca
     1081 gtgccacgcc attcatgcag tagcgcaggc cggtgggctt ggggccgtcg tcgaacacat
     1141 ggcccaggtg gctgccgcag cggcggcaca ggacttccac gcgccgcatg ccgaggctgg
     1201 aatccacctt ttcgtccacc gccttgtcca gcggttgcca gaagctgggc cagccggttc
     1261 cgctgttgaa tttggtgctg gacgaaaaca gcggcaagcc gcagccggcg cagttgaaga
     1321 caccggcgcg tttctccttg tccagagggc tggagtacgg gcgttcggtc ccggccctgc
     1381 gcagcacctg gtactgctca ttgctgagca gcttgcgcca ttcggcgtcg gtgcgcgtca
     1441 gctcgtatga ccgggtgggc tgagcggcct cggccgggcc gctggcgaac agcccacgga
     1501 ccccggcggc gaggcccgac aggattccga gacccagcag cgtattgcgt cgattgctca
     1561 tggggctgct ccttgttcgc gcattgtgga cctgcacggt agtcggcggg gcggcgccgt
     1621 tcttacaacc atattcggtt cataggtcac ccccggcgct ggccggttgc ggcgtcgggc
     1681 agagcctcga atgccggcgg cgcctgtcaa cgcaaagtag aaatgtccct tttctccaaa
     1741 atagaaatgt cccttttctt ttgtcgagca cgggacagtc tgtggacgga ttgcttcttt
     1801 ttcactccag cggctgtttg gcaagcgaag cgcgtcaggc cgaaggctcc aagccgggga
     1861 gcggtgggta actgacgcac cggagtattc gccggctgtc cacaccttca gggtgtgggc
     1921 aggtcagtta tccacggcgg gcggaatcgg cagggatcaa gggtggctgt tgcacatgct
     1981 cgttcaggct ggagccgaag cgatgaatga aaggcgcggc accgatgtgc agggcggtct
     2041 tgtccagctg cgccgcacca gggcagtggg cctgcagact ttgcgcaacg acccgcagga
     2101 agatgcgcag caccatgttg agcaccaccc cgtcgcgctg catgaagtag cgcagtcgtt
     2161 tgataaagca ctatgatgta ttcgacaacg cttttccaca gtacaggatt cgatcgacgt
     2221 tgcatatgag cactacactt actcatccgc tgagtcttgg aagttctgga acagcggttc
     2281 gtgccctgca aacacaactc aatgcatcac aactggcact aggaccaaaa ctttctgttg
     2341 atggcagttt tggcaaagtg acgcacatcg cggtgatcgc gttccagcgg cggaaaggcc
     2401 ttaaggctga cggcgtcgtc ggggcgaaga ctgcaagggc cctggcctgg aattatcaac
     2461 cggtgaatga aaagccctac atcgtaaggt atgacaaacc gcccctgcct gctgcgactc
     2521 cgccgctcgc ggtcgttgcg gaagctatac gcgcgggcat ggacccatta acggagaagc
     2581 tcgccgacga cctttgggta gcttttaacg acccgaacaa cgatcctaat tatcgaacac
     2641 taatgggcaa ggctcacgcg cacgatcctt ctctttcgca gaaacagaac cggcagatca
     2701 ttctccggat caaagatcta atttttcatt acaagaagtt ttcggaagtc ctgagtcaat
     2761 tgggagatct atcagcgagt gatccggaaa tcgtaccttc acgtctgcga ggcgcattca
     2821 aggaattcgt aaaccaaatc atttcagcgt gcaacgctat ggatttttat tacggaatca
     2881 cggaacactg tcgtaaacgg ctgaaccgtc ttccatatga aagcattgtc ggcaaagttg
     2941 aaagcgtact gaaaggcgac cgcaccgtta cgtttgccgt agctgaaata caaatgctgt
     3001 ttcagacaat ggcatacgcc gccgtatttg atcggtcaaa agtggttgac cggccgactc
     3061 tcgattggct ggaacggttc gaataccacc ttaagcctaa ctttaattaa gtcgtgtctt
     3121 gcccgcgcag tagcgcagga agaggcactg cacttcacgc tcgtgcgcga tcaccttgcg
     3181 tcgctgggtt atgcgtacgg tgatttcccc gtgcacaacg ccttgtggga gatggccgag
     3241 aggactcgcg acgacgtgct ggcgcgcatg gcgctggtgc cacgcacgtt ggaggcgcgc
     3301 ggcctggacg cctcgccgcc catgaaagcg aaattccttg cagcaggcga cgtcgcggcc
     3361 gccggcatcc tggacgcgga aggacagatc tcgaactggc agggtacgtt gtgggacctc
     3421 agtgagcacc ctgacctggg cagttgaagt caggcggtga agtgatgggc ctgtcgttcc
     3481 gtcgatttct gattgctcag gacggaagcc tccatcacct ggctgcgcta aatgtctgct
     3541 gctgccgacg gcgcctgcgt gcggcggccg gaaaatggcc gatggcgggc acatagggag
     3601 ctatattccg taaggccaac ggtcggcggc gccttgccca accgtttgct gcgggctacc
     3661 atcgcttgcg cccgtatgcc cgtccaggtt cctccggagg cttccatgcc gcaactcatc
     3721 gcgtcggtcg aaggtgtcca gatcaagcac gtgtacctgc agcaggaccg cacgaccctg
     3781 gggcgccatc ccgacaacga catcgtgctg gacaacatgg tggtcagcag ccaccactgc
     3841 gtgttcgaac tgaaggggct ggcggacgtg ttcatcgagg acctgcgcag caccaacggc
     3901 acctacatca acggcaagat ggtcaagcgc cagaggctgc acgacggcga agtcatcgcc
     3961 atcggcaact tccgcatcca gtacctcgcg gaatccgaac ggcccagcgg attttcatcc
     4021 agcggttttt cccataccac ggcgatggag ctcgatgcgc aaggagttcc ggtcgcgcac
     4081 gccttcttcc gggtcctgac gggctcgtcg gcggggctgg aggtgccggt cgtcaaggcc
     4141 gtctccacct tcggcaaacc gggcgtatcc gtggtggccg tgtcgcaccg gcgggacggc
     4201 ttctatgtgg cgcacctgga cggcgacgcg ccgccaactt tgaacggcga ccgcatcggt
     4261 tcccaggcgg tgatgctgtc caaccacgac gtggtggaac tcgccggcac caggatgctg
     4321 ttccagctga aagagcagcc gtaggagtct gcagcgataa tgcgcttcat gggatacgcg
     4381 gtcggcgact tatgggaatg ctgaagaaat ttttcggcgg gggccgcggc gccggcgaat
     4441 cgcagccgcc gcattcgtcg cagttccacg aaagcgaaac cacggcgcag catcacggct
     4501 cgcgcaacgc gccgcgccgc gagctggtgc aattggtgct gcgcgacacc atgcgcaagc
     4561 acggcattcc ttccgactgg atcgactgcc gcatcctttc ggtggtcacc cgcaggcaca
     4621 tgtcgggcat gcacgtgcag ttcatcgtcg acaagggcca gtcgcgcctg ctcgcttacg
     4681 tgcagccgtt ccaggagagc ttctgggagg aaatcgagaa gttcgagccg catgcccggg
     4741 attggctgtt cagcctggcc tggcaattcg acggccccgg cactcgcggc aggtccatgc
     4801 ccggcccggg cggatggtat ggcgacgagg cacgcgcagg cggcgacacc cagtcacccg
     4861 acgcggacgc ggacgaactg gcgtccgact tgcaggcgct ctatgccatc cgggacgcgg
     4921 cactgtcccc gccggcggaa cgcacggcgc cgcaaggtgg acccgagcct tcaagccgcc
     4981 ccggcggccc gcccgactcc tgaaaccggc ttgacttggg ccacggccgc cccatactgg
     5041 agcgtccaac ggacggaggc gcaagatgtc tggcgtgagc atatcggacc ctgtcgcgaa
     5101 agaagatggg gtcgaattca ccgcaacccg aggcggcgtc gaacagaaat tcctggcgca
     5161 ccgcgaagcc ttgcaagacc tggactacag cgttttcgaa accgcggagg cactgctgaa
     5221 ggccttccac cggcaccagg gccagatcgc cgccatcgcc ggcagagcga tggaccaggg
     5281 cgtcggcgcc ggcaccacgg tagtactgca gagcctgctc taggcccgac gcgcccatgc
     5341 ggtgggcctt gcattcgggg ccatcagccc cagcttcgat gcatgaaccc taccccgttc
     5401 cttacgctac cgggccaagc ctgaggacgc cacatcatga gcgaagcgct gcatgtcgtg
     5461 tgcccgcact gccataccac caatcgggtc gggcagggcg acctggccaa ggcgccggat
     5521 tgcggcaact gccacaagcc gctgttcacg gcccgttcga cgcccctgga cgaagccgct
     5581 ttcgagcgcc atatcgcccg caacgacatt ccggtgctgg tcgatttctg ggcgccgtgg
     5641 tgcggcccct gccggcagat ggcgccggct ttcgagcagg ccgccgcgca gctggagccg
     5701 aaggtgcgcg tcgccaaggt caacaccgag gaggcgcagt cgcttggcgc gcgccatcgc
     5761 atccgcagta ttcccacact ggcgttgttc atgaaaggcc gcgaggtcgc gcgacaggcc
     5821 ggcgcgatgg gcgcggccga catcgtgcgc tggacgcagg cgcaactgcg ctgaacgcaa
     5881 gaacagtgcg agcaggccct aattgaagcg ctcgagcacc tggtagcctg ccccgccggg
     5941 cagcgagcgc accagcacat aacccgagtc gatatgccag cgcaaggcgg gcccttgcgg
     6001 cggcggcctg gcgcccttgg cgcgccgcgc cagcgtgacg tgcgcgagat acggccgcgt
     6061 ttccaccgga aggtccaggt cgacaagccg gtggcgcaag gccgcgtgca gccgcaggag
     6121 cgcaggcggg ttggcagcgg accgaagcac ggccacgccg ttggggaaga cctcgccctg
     6181 atccagttcc agggtgaagg actcgaaggg aacccgcagc gcccgcgtca ggtgcagcaa
     6241 gcgatccgcg ggcacatccc cgaggaaatg caaggtgatg tgcagccgct cgggcttcac
     6301 gcgcgcggcc cgtggcggcc agtcccaggc ctgttgccag gacgcgatgg ccaggcgcac
     6361 ttcctctttg ggccataacg cgacgaacag ccgcagtggc ttgccggcat cgggggcgac
     6421 agcttgcatg gagccaattt tgcgcgcgcg atacgcggcc acatattccc tagaatggcc
     6481 gcaacgatga caaaagccga cattccggac gaggtccggc gcttcatcct gaccagcgtg
     6541 ccttccgtgc cttacctcga ggccatcatc ctgttgcgcg ccgagccaaa aggtccctgg
     6601 gacgcgcaac gcctgtcgcg ccgactttac ctgcctgagc cgcggacggc cgaactgttg
     6661 caggcgttgg ccgcggcagg catcgcggag ccggcgcagc aagaggcatc gcagcgggag
     6721 agcgaagctt tccggttcaa acccgctacg cccgagctgg acgccatgct cgatcaactc
     6781 acccgcgtct acgccggcga cctgatgggc gtgaccgacc tgatccattc ccgcgtcgac
     6841 aagcgcgcac agcagttcgc ggacgcgttc cgctggcgaa aggactcatg acatggccgc
     6901 atttatctat tcgctatgtg cgttgacgtg cctggccgcc ttcgggctgc tgctgcgctc
     6961 gtggcgggcc acgagacatc ggctgctgtt ctggagcgcg ctgtgctttg ccttcctgag
     7021 cctgaacaac atcctgctgg tggtggacaa cctggtgttg ccgcaggtcg atctatcgac
     7081 gccacggctc ctgtccgcct tgctgggcgt actgctgctg ctgttcggcc tggtctggga
     7141 agaggagtag gcagcgatga accagatgct catgggcgcc attgcggtgg cttcgttgct
     7201 ggcgagcctt ttcttcctgc gtttctggcg ccatacgcgc gaccgctttt tcctgtattt
     7261 cgccctctcg ttcgcgatcg aggggttcaa tcgcctggtg atgggactcc tcgacgcaac
     7321 gcgcgaggac aaccctgtct tctacagcgt ccgcatcgtg gcctatgggt tgatcgtggc
     7381 agccatctgg cagaaaaacc ggccgcgccc gtaacaaacc cgatcaggcg agccaggcca
     7441 ggaactcgtc gagcaaccgg ttgaactcca gcggcttgtc catgtgcggc cagtggctcg
     7501 tgccgatgat gaccttgcgc gacacgtcgg gcaattgccg gtgcaaggcc gtggggctgt
     7561 cgccgtgtgg cgtatcgatg atcagcttgg ggcccggata gcggcccagg tcggggaggg
     7621 gatcgaagtc gaagatcgcg ccgatcatcg cgagcgacgg ctcgcgcccg acgctgcgca
     7681 tcccggcgtc cagcaactcg cgcacgggtg gctcggcccc ttggagcagg gaactccaat
     7741 agccggccat cacgttgtcg tagtcggcgc gcaacgcgtc catgatcttc tgcgcttcgc
     7801 tcgcggagga cttacccggt gaacctgcca gcaccagccc ggcaacccgg cgcgggtgtc
     7861 tgccggcata ggccgacgcg gccgcaccgc ccatgctgtg gccgaccagg acgaagcgac
     7921 tgagctcgag cgcgtcggcc accgctgcga tgtcctcggc caatgcggga acgctgtggt
     7981 cgccggatgc cggggcatcg gagcggccat ggccgcgcaa gtccatcgcc agcgcgcggc
     8041 ggcgtcgcct gagatgggcc aattgcgcct tccagtgcga gctgctgccc gcataggagt
     8101 gaacgagcag caccggcagg gcgcctgcgc cgccgtcgtc gacatggagc tggccggccg
     8161 ggcccgcgat cgttgtcatg gattggctcc ttcagagctt gggaatgtgc agcgtcttgc
     8221 tgatcatcaa gctgccggaa agcacgaaca gcagtgccag cggatgcagc acccaggggc
     8281 cgatggtgta ggcgccgccg taaagctcgt cgcccagccg tccctgcgcc gccgcgaatg
     8341 ccagcagccc ggtgagtact acgctggtgg gaatcggcgt gccttcgaaa tacgacacct
     8401 tgtcgctgcc cgccgaaagt tcctcggccg tcacgttgta gcgggcgagc cggctcacac
     8461 cgcagcagac gaaaaagatc agcgcggcca cgtcccaccc cccctgaagg ccggcggcga
     8521 atccaagcgc cgcgggcgcg acgccgaacg agatcacgtc ggacagggaa tcgagttcgc
     8581 gacccagtgg ggaatgctca tggcgagcgc gggcgatgcg cccgtccagc acgtcgaaga
     8641 tgaaagcagc cggggcgagc gccgcggccg cgtagaaatg caagggggat tcgccggcga
     8701 cgaagagcat tgcgaagaac accgcgccca cgccgcaggc cgcgttgccc agcgtgaaga
     8761 aatcggccac atgaaaaccc cgcagcatcg agaagtggcg gggagcggct tgttgcatgc
     8821 gcggtccttc gaagtgtcga gctcacctta agcgcggcgg ctcgcctttc ccgtcggacc
     8881 cgggcacgcg cgcctgtcgg cgctgtccga cgcccacggc ggagctgccg gcgcaaaaaa
     8941 aagaagggcc cgtgcgaacc cttcctgcaa cgcgtggcga cggcgggtca gccgccggcc
     9001 ttcaggtaca tcaggatttc cttgaactgg tcggcgttca tcgcttcgcc cttcgcgccc
     9061 atcttgcgcg cgttcatgtc ccaggtgaga ttcatcatgt cgatgaattc ctttttcgag
     9121 accatgccgt ccttgttctt gtccagcatc ttcatggaca tgatctcctg gaatgggtag
     9181 tactcggcgt tggtggggcc cgcggcgaat gcggttcccg cggccagcag cgtggccgcg
     9241 agggcgatgc gcgcggcggt ttggttcggt ttcatgggaa agactccggt agtgaggtga
     9301 atgaggatga ccagggccct ggcgggccct tcgcggcgag ggccgcgcgc cggcgtgtca
     9361 gccaccggcc ctcaggtaca tcaggatttc gctggaggac tgggcatccg aggacgcatg
     9421 accctgggcg cccatcgtct tcgtgtcgcc attcgtggat ttcgtcgtgg acttcatcgc
     9481 ggtgctgctg tcgatgactt tgaccgacga cgtcgtttcc tggaagggat agaactcggc
     9541 gttggtggga cccgccgcga tggcggtgcc cgcggccagc agcgtggcgg cgaaggtgag
     9601 ggcggcggcg acttggttcg gcttcatggt gactctccaa tcaatacaag aggtggaaga
     9661 caaggcgggc atcggaattg atgcctgccg catcggaagg aaggtgttcg cctcgtgtcc
     9721 gatgtgcgtg tcagtcgccg ggcgagcggc ttgcttaccg gcggaatggg cgttagtcgc
     9781 agcgtccgtg aaatacgcca cacaacctgg cactatcttc ggtatgggtg ccggcggcac
     9841 aggctcagcc gtgtgcgcca tgccgggcac cgcgcaccgc gtaccattca tgttccgtcg
     9901 ggttcgaaga aagaggcata tggacatcga gcaattcatg caaggctaca agcgggcctg
     9961 ggaaggcagg gacccagcgc tcttctgtgc cctgttcgcg caggacggcg tatatcgcaa
    10021 cacgccattc gcggtgcagc gtgggcatgc gcagctcgcg cagtattggg agcgagtgaa
    10081 gctgcaggag gatgtgaagg tcctgttcga tgtgctggcg tcggcgccgg ccagcggtat
    10141 cgcgcattgg cgcgtgacgt accaggtggc gtccgaggag ctgttcagga tctgggccgc
    10201 gtccacgggc acgaacctgg tcgcgcgcaa gccgggcgat ccgcttccgc gcatggttct
    10261 cgatggtgtg ctgcacgctc gtttctcagg tgagctgtgc agtgaatgca ggatctggtg
    10321 gcacagcatg cctgtgccgg cgtaagaagt cgattgttgt gccggcgagg tgatagattc
    10381 atggcgtgac gagcacatcc aagatccttg ccgccctgct gggcggctac gtcctgctcg
    10441 tcgtgcccgc gtacgtcggg cccgcgtttc tcgaagagtt cagcggcttt ttcgtgctgg
    10501 tgccgctcct gtcgatctac ctttttcaca aggtcggcgt tccggggctg ctcgagcatg
    10561 gcggtgcctg cggctggggc tggtgcgcgc cgactgtttt cgggtggata ttcgcggcgc
    10621 tgttctggac cggactcgcc tggctggcgg ccgcgggcct ggcacgcctg atctcacgct
    10681 tttcaagatg aggctggcgg cgcttaccgg cgcacgcgcc gccttgcggc tgctcgctta
    10741 tgcctgggcg gcgccatact cgctactggg tctgactgtc gggctgctgg ccctgctatt
    10801 gggcgcccgc gcccgcttgc gcgaaggcgc gatggagttt ggcggcggcc ggctgggcgt
    10861 gcggctgtcg cgcttgcccg cgcccttctg tttttccgcg atcacggtcg gccacgtgat
    10921 cctgggcatc gatgaggcaa cgctcgccgc cgcacgggcg cacgagcacg ttcatgtgcg
    10981 gcaatacgag cgttgggggc cgctcttcgt gccggcgtac ctgctgtcga gcctggcgca
    11041 actcgtgcgc ggccggcgtc cctacctcga caatcgcttc gagcgcgagg cgtattccgt
    11101 ggccggctgc ggacccggcg cagcgcaggg gcggtctttg ccaaagccgg gtgaacagcg
    11161 gtagcattcg tctcccctga agagccgggc gccgcaagac gccggttgaa gccgcccaag
    11221 gagactcacg atgaaggccg acgatttgcg ggcgctgcag tcgccgctga aggagcgtta
    11281 tcgcaacgag cccgcggcgg cgctcatcac gctcagcgcg caaggacggt tgggcgaagg
    11341 agtgacctgc cggatcgaga ccggcaaggc tctggtcgcc gcggggctgc atccggcgac
    11401 aggcggtacc ggcatgagcg cttgttccgg cgacatgctg ctggaggcgc tggtcgcgtg
    11461 cgcgggggtc accatgaatg ccgtggcaac ttcccttggc atcgagctgc gggatgccgc
    11521 ggtacacgcc gagggcgatc tggacttccg tggcaccctg ggtgtttcca gggaaacgcc
    11581 ggtcggattc caggccattc ggctgcggtt cacgctggac accgatgcat cgcaggaaca
    11641 gctgtcgacg ttggtgcgcc tgacggaaaa atattgcgtc gtgtaccaga cgctggccca
    11701 ttcgccggcc ctgggcgtga cgaccgaggt cggccgcgtc gcgccggtgt aaagcggttg
    11761 gcaaaggcac gatcatggat tccgccccga cctcacccgt gcggcatgcc ggcggtttct
    11821 acgagaaccg catttttccc tggctcaacg accggctcgc gcgcgacccg gaactcgagc
    11881 gcacccgcgg cgaagctctc gcgcccgcgc gcggacgggt catcgaaatc ggctttggaa
    11941 gcgggctgaa cctgcgtcac tatccggcgg cggtgcaatc gattgtggcc gtggagccca
    12001 acgccgggat gcacgagcgc gcggctgggc ggatcaaggc gtcgcgcatt ccggtcgagg
    12061 tcgtcatggg caccgccgag agcatgccct tgcccgccgg cgcgttcgac accgccgtca
    12121 gcgtcctgac catgtgctcc gtcgccgacc cggtgcgtgt gctgtcggag cttcggcgcg
    12181 tgctgcgcga tgacggacgg ctgctggtgt tggagcacgg cctcgccgac gaacccggcg
    12241 tcgcgcgctg gcagaaccgc ctgaacggca tccagcgcac cgtgggctgc ggctgcaacc
    12301 tcaatcgacc catggcgcgg ctggtggaag gccacggctt ccgcttcgaa gccgtgcgca
    12361 ggttctacgc gccgaaaata ccccgcaccc acggctggct gaccgtgggc acggctgtca
    12421 aggcctgaag aaatttttcg ccccacctgt cgaaatggag cgggctggcg cgtcattgtc
    12481 atagcggcgc atgatgcccg cgacaaccca acctggagcc cgacatgcaa tacatcctgc
    12541 tcatctacgg caacgaagcc gccatgcaat ccgcgaccga atcgcaggtg gcgcagatgg
    12601 tcgccgccta cggcgcctat agcgaggccc tgaagaaggc cggcgtgatc gtggggggcg
    12661 aacgcctgaa gcccgtttct tcggccacca ccgtgcgcgt gaccggcggc aagaccgaag
    12721 tgctcaacgg gccctatgct gaaaccaagg agcaactggg cggctattac ctgatcgacg
    12781 tgcccgacct ggacgcggcg ctgtcctggg ccgcccgctg ccccggtgcc aatgccggca
    12841 cgatcgaggt gcgcccgatc tgggccatgt agccggatga cgcacgccat ggtcgcgcgg
    12901 gaagccgccg aaggtgcggc acgccgcagc tatggcaagc tggtggcctt cctcgcggcg
    12961 cgaacgcgcg atgtggcggg cgccgaggac gcgctggctg aagcgctcgc gacggccctg
    13021 gccacctggc cggcgcgcgg cgtgccggac aaccccgagg cgtggctgct cacggtggcg
    13081 cggcgcaaga tgatcgacgc gatgcgcgaa cggcaacagg gcgagggcgc cgccgaacac
    13141 ttcgcgctgc tggccgaact ggcggagggc gaggaaaacg acgcgactat tccggacgac
    13201 cggctggcgc tcatgttcgc ctgcgcccat ccggcgatcg aggcgggcat ccgcgcgccg
    13261 ctgatgctgc agaccatcct gggcttcgac gcggccgcga tcgcctcggc cttcctggtt
    13321 tcgcccgcga ccatgggcca gcggctggtg cgggcaaaga gccgcatccg ccaggccggc
    13381 atcccgttcc gcgttccggg gcgggccgag ctgcgcgagc ggctggtccc cgtgctcgac
    13441 gccatctatg ccgcttacgc cgaaggctgg gcggacgcgg cgggtaccga cgcgcgccgg
    13501 cgcaacctgg ccgaggaagc gatctggctg ggcggcctgc tcgcatccat gctgccgcag
    13561 gaacccgagg cactgggcct gctgtcgctc atgctgcatg cgcaggctcg ccgccgggcc
    13621 aggcgcgacg ccgacggtgc ctatgtgccc ctcgatgagc aggacgtggc gctgtgggac
    13681 gaacgactca tcgtcgaagc cgaggccctg ctgcggcggg ccagcgcgct ggaggggatc
    13741 ggccgcttcc agctggaagc agccgttcaa tccgctcacg tggtgcgccg gcgcgacggt
    13801 cgcgcggact gggcggcgat cgagcggctc tacgaagcgc tggagacgct gaccgcgtcg
    13861 ccggtggtga gcatcaaccg cgcgattgcc attgcccaaa cgcaagggcc gcaagccggc
    13921 ctgcaactgc tcggcagcct ggccgccgac gtgcgtctgg ccgactacca gccttactgg
    13981 gcggctcgcg cgggcctgct cgcgcgcacc ggcgacatcc cgcaggccct cagctcgtac
    14041 gagcgcgcga tcggactgga gcgcgacccg gcggtgcggc agttcctgca gcggcagcgc
    14101 gagaaattga cggggcgata ggacactaac atacgcgcat ggcgcgccgc caccactacc
    14161 acgtctacgt cgtcgaactg tccgaccagg tgtggaacgt gggccgcttc cggcgcgcga
    14221 atccgcatta ccagctgggc aagccgttcg tctatgtcgg catgaccggc ctggatccgg
    14281 acgtgcgcct ggacaagcac aaggccggca ttcaggccaa cagcttcgta cgcgactacg
    14341 gcctgcgcct gctgccgggg ctgtatgccg tctacaaccc catgccctac gatgccgcgc
    14401 gcgagatgga ggtggaactg ggcatcgcgt tgcgcgaggc gggctacggc gtctggcagg
    14461 cgtaggagcc tgggcggcag agaaacgggc ctgcgttact gccggatctt gccggcggtg
    14521 ctgatgatgg acagctcgac gaacttggag cccgagcgca tcccgggctt gaatccgacg
    14581 gcgtagccgc ccatgttgta gttatccatc cgatccagcg cgctgaccat gccttcgcgg
    14641 ctcggacgcg cgccctggcg gcgcacggct tccacgatca ccttggcagc gatgtagccc
    14701 tccatcatcg cgtagctcac cgggacctcg agcttctcgg tcccggcgac cgcgtcagtg
    14761 aactccttgg acagccggct ggaaatcttg tagggactcg gcgtgacctg cgcgatggcg
    14821 acaccctgca tctgctcttc ggccagccgc ttcgacagct gttctatgtc ggcgcccgaa
    14881 tgggcaaata gttgggccgt gccgcccgcg gagcggtatt cctcgatgaa ccctgccgcg
    14941 gcggcgccgc tggacaccat gatgatggct tgtggcgggg tggccatgaa tgtggcgatg
    15001 gcgggcttca cgcgcgccgt gccggtcgcg taggaggcct tggaaatgat ggtcgcattg
    15061 cccttcctgg cggcttgatc ggccgcggcg aggagcgccg gcgcgccggg accttcctca
    15121 tagagcagcc ccaggcgctg gatgccgatg gtggccaggt gctgggtcag cttgttgatc
    15181 tcgtcgcgca ggccggcgcg aacgctgtac agcaacgggg tctcggggcg gatctcggcc
    15241 gaccggtagc ccaccagcgc gatcctgtct ttcgcaagca cgccggaact gaccaggtcg
    15301 gtgatgttgc ggctgccgaa gaagcccgcc agcaccatcg gcttttcttc cgccagcaat
    15361 tgccgggtgg cgctgacggt gtcctccggg cgcccgccgt cgtccttgcg caccagggtg
    15421 aaggtgtggc cgttgacgcc gcccgccttg ttgacgccgg tgaacagcag ctgcatgccg
    15481 gcagcgtagg cacggccctg gttggcttcc agcccggaca tcggtcccac ctgtccgacg
    15541 acgatttgcg cggcctgtcc gagttgcgcc atcgcgaggg cggcaccggc cagggccatg
    15601 cgtgctaccc gattcatcgt tgtcattgaa acccccacga atagttaagg taactattgt
    15661 ccgaccatat gccacgtacg gccgccgcgg caatgtggaa agcacttatg ccgctaggaa
    15721 aaaacggcgt ttctcatgtc caataccgcg atgacaacgc ttgccgcgac ccggaaggac
    15781 attgaatcgc cttatgcctg gatgcggctg gccgtgtcac tgttgctcat gaccatcggc
    15841 ggctcgggca tgtattcggt cacggtggtg ttgcccgcca tccaagcgga gttcgctgtc
    15901 gcgcgcgcgg acgcctcact gccttatacg ctgaccatga tcggcttcgg gctgggcggc
    15961 attctcatgg ggcgcctggc cgatcgattc ggcgtgctgg taccggtggg gaccggcgcc
    16021 ctcggcctgg gcgccggctt cgtcctcgcg gggctggcgc ccaacctgtg gcttttttgc
    16081 gccgcgcagg ggctgctgat cggcttgctg gggacttcgg ccaccttcgc gccgctggtg
    16141 gcggatactt cccagtggtt cgaccggcgg cgcggcatcg cgctggcgat ctgcatgagc
    16201 ggcaattaca ccgctggcgc cgcatggccg ccggtgatgc agcacttcat cgacgccgtg
    16261 ggctggcgcc agacgtacat cggcatgggc gtcttctgtg tgctgacgat gctgccgttg
    16321 gcgctggtgt tgcggcgccg tccgccggtg cacgcgcacg atgcgacagc ggataccgtg
    16381 acatcgcgcc cggcaacgca gaccgcttcc gatcggccca tgggcttgag gccgggtacc
    16441 gtgcaggctc tgttgtgcgt ggccggcgtt tcctgctgcg tcgccatgtc catgccgcag
    16501 gtccacatcg tggcctattg cgccgacttg ggctatggcg cggcgcgcgg cgcggagatg
    16561 ctggcgctga tgctcgggtt gggcgtggtg agccgcctgc tgtcaggatg gatttccgac
    16621 cacatcggcg gcctgcgcac cctgctgctc ggctccatcc tgcagggcgt ggcgctgctg
    16681 ctgttcctgc cgttcgatgg gctggtgtct ctgtacgtgg tggccggcct gttcggactg
    16741 ttccagggcg gcatcgtgcc ggcctatgcg ctgatcgtgc gcgagcattt ttcgccgcgc
    16801 gaggcgggtg cgcgcgtggg gatggtgctg atggccacgc tgttcgggat ggcgttgggc
    16861 ggatggatgt ccggcgccgt gttcgacctg accggctcgt accgcgccgc gttcatcaac
    16921 ggcatcgcgt ggaacctgct gaatgtcgcg atcgtggttt tcctgctgta tcgggtgcgg
    16981 gccgtggctc gcggcaggcg tcaattgtcc ggcgccccga gttgatatag tgaagcggcc
    17041 atggcccagc atcgcccgat cgatcgccgc cgcttgctgc agctggcggc ggcgggtgcc
    17101 acgagcctgt ggctgccgca ggccgcccgc ggccagccgc ggctgaccgg caacccgttc
    17161 gccctgggca tcgccagcgg ttctcccacc cacgactccg tggttctgtg gacgcggctg
    17221 gtcgcgccgg gctttttcga gacgctcggt gagcaagcca ttcaagtgcg ctgggaagtg
    17281 gcgcacgacg agcaattcag ccgcgtcgtg cggcgcggcc agtccccggc gttgttcgag
    17341 ctggcccact cggtgcacgt cgaagtggcc ggcctggagc cggaccgatg gtatttctat
    17401 cgcttcatcg ccggcgacgc ggcgagcccg accgggcgga cccgcacctt tgccgcgccg
    17461 gatgcggcag tgcaaaagct ccgtctggcc tatgcgtcct gccagcgctg ggaacacggc
    17521 tactacagcg cttaccgcca catgcgcgat gaaaacctcg acgccgtgct gttcctgggc
    17581 gactacatct acgagtatcc gatcgccgcc aacccggtgc gggtgccaac cggcggctgg
    17641 gtcctcacgc tggaagacta ccgccggcgc tatgcgctgc acaagagcga cgccgatctg
    17701 caggcgatgc acatggcgtg cccgtggctg cacatctggg acgatcatga agtgcacaac
    17761 gactacgccg gcctgaagcc tggcgacagc gggcggctcg cgccgctccc gatggacggt
    17821 ttcgtcgccc gcagggccgc ggcgtaccag gccttttacg aacacatgcc gctgcgcgcg
    17881 tcggtgttga cccgagcggt ggcggggctg gcgtcaggcg ccgaaatgcg catctactcg
    17941 cggcagcgcg tcggccggct ggccacgatc ctgatgctgg acgcccgcca gtacaaagac
    18001 ccgcaggtct gcatgccccc cggcacgttc gggtcggctt cgatggaccc ggccgcctgt
    18061 cccgattgga acgatccgaa gcgcagcatg ctgggcgcgg tgcaggagca atggctggag
    18121 gcggccttgg acggcagcac cggctggacg gtgatcggcc agcagacgct gttcggccgg
    18181 cgcgacgcca agcctggctc ggatgagatt ttctggaacg acggctggga tggttatggc
    18241 gccgctcgga cccgcctgac gaatgcgctg cagctgcatc gcgtggccaa tccggtgatc
    18301 ctgggcgggg acgtgcacca gaactgggtc ggcaacgtca aggccgacta tgcgcgtgcc
    18361 gacagcgcca cagtgggcgt ggagttttgc ggcaccagca tctcgtcgcg ctcggcggcc
    18421 aacccgagga ttcccgagcg gctggccgag aacccgcact tcgtctttgc cgacgccgag
    18481 cgcaggggct atggcgtggc cgagttcacg cccgcgcggc tcaccaccac cttgcgcgtg
    18541 gtcgatgacg tgcggcgcag ggattcgggt gtccagacgc tggccagctt cgtggtggaa
    18601 gccggccgtc ccgggttgga aaagtcatga ggcgccgctg gtggtggtgc ctctcctgcc
    18661 tgctggccct gataccggcg gcggcccagt cgccgccggg cgaactcacg atccgcggcg
    18721 acgacagcta cgtgctgtcg catgccttcg tctacctgga agacaagggc gggcagctga
    18781 cgctggacga cgtcctgaag ccggaggcaa gcgcggcatt ccggcccttg ccattgaccg
    18841 gccccggcgc caatttcggc atgacctctt cggccatctg gctgcgcatc acgctgaagg
    18901 ccttgccgac ggccgcgccg gactggctgc tggagctggc gcatccaccg ctggaccggg
    18961 tggagctgtt ctcggccaac gcgtccggat cgcagcggcg cttgggcggc gacctgcagc
    19021 cattcggcag ccgcgccgtg ccccaccgca atcacgtgct gcccgtgacc ctggcaccgg
    19081 gcgcgcaaac cgttctccac ctgcgcctga agtccgaagg caccgtcgtg gcgccggtga
    19141 cgctgtggcg gccggccgca ttgtggcggc acgatcaggc cgcgtactcg gtgctgagcc
    19201 tgtatttcgg cctgctgatc ggcctgctgc tctataacct gctgctgttc gtctcggtgc
    19261 gcgacgtcgg ctacctcagc tacgttgcct tcgtcgcctg tatgggcatc gcgcaggccg
    19321 cgttgacggg attgggcgcg cagttcctgt ggccgcaatg gacctggtgg aacagcgtct
    19381 cgcccctcgc cggcatagcc gccgcggcgg gctttgccgt cctgttcgcg cgcaatttcc
    19441 tgtccagccc gatccgcttg cgccgaatgg acaggctcat gcttgcgcaa ttggcgggct
    19501 ggagcctggc gctgctggcg gcgctggcct tgccgtatgt cgtatcgctc tggatggtca
    19561 ccatcctcgg cgtgctgggc gtcgccacca tgctggtggt cggcgtgatc agcgtccgcc
    19621 gcgaattcgc cggtgcgcgc tatttcttca ccgcctgggc cgtgctgctg ctgggcgtgc
    19681 tgacgctggc actgcacagc gccggcatcg tgccgtcgaa tgccattacc tccaacgcgc
    19741 tgctgatcgg atcggccctg gaaatggtgc tgctgtcgtt cgcgctggcc gaccgcatca
    19801 acgtggcacg ccgcttcaag gagcaggccc aggcccggat cgccgccgaa cgcgccatgg
    19861 tcgaagcgtt gagccagtcc cacgggcaac tgcgcatgat gctggatgag cgcgagctca
    19921 tcctcgaaag ctccatcgtc ggcatcgcgt tcctcacgcc agagggccgt tttcgctggg
    19981 ccaacaaggc gatgatcgaa atcttcggga cctcgttccg cgcgaatacg tcgatggagc
    20041 cgttctacct gtcgcgcgag cagtacctgc aggttggcgg cgaggtttcc gctacggtag
    20101 gccgcggcga ggtgtatgag acggagctgc ggatgcggcg cgcggacggc acgccgatct
    20161 gggtctcatt gtcaggcaag gcggtgagcc ggcgcgacct gggccagggc acggtgtggg
    20221 tcgtcatgga catcacgcga cgcaaggaac tcgaggagca gttggagcgc agcagttccg
    20281 atcaggcccg gatccgcgag gcgctggagc agcagaaagc gtgagcgccg cgttcggcgc
    20341 agcacctgga gcaatggtcc cggccgccag cggtgatcgc ggcggtcgcg tgctcgcgta
    20401 cttttggctt gcctgacgag gctcacggga cgccggccga ctcgccggtg cccctgtcgg
    20461 gggtaaacgt gggttgatgc gaattgacag gggttccata ctcatttccc atttatccaa
    20521 tccagatcga ggagttgcca tcatgagtct tcagaccaag tatctgctgg acgaaaacca
    20581 gatgcccaaa ttttggtaca acatccaggc cgacctgccc actgccgcac cggccgtgtt
    20641 gcacccgggc acgctgcagc ccgtaggccc gaacgatctg gcgccgctgt ttcccatgga
    20701 actcatcatg caagaggtga gcacacagcg cgaaatcgag attcccgagc ctgtgcgcga
    20761 tgtctatcgc gcctggcgtc cggcgccgct gtaccgggcg caccggctgg agaaggctct
    20821 gggtacaccg gcaaagattt tttacaagta tgagggagtc agtcccgcgg ggagccacaa
    20881 gcccaacacc gcggtggcgc aggctttcta taaccagcaa gccggcgtca agcgcctggt
    20941 caccgaaacc ggagccggcc agtgggggac ggcgctgtct tatgccgggg cactgttcgg
    21001 ccttgaggtg gatgtctacc aggtgcgcgt gtcctacgac cagaaaccct atcgccgcgc
    21061 cgtgatggag acctatggcg cgcgctgcgt ggcgtcgccc tccaacgaaa ccgtctatgg
    21121 ccgttccgtg ctggccaaga ggccggatca ccccggcagc ctggggatcg cgatctcgga
    21181 ggcggtcgaa atggcggtgc agcgcgaaga caccaagtac gcgctgggtt cggtgctgaa
    21241 ccacgtgctg ctgcaccaga ccatcatcgg cctggaggcg atggagcaga tgcagatggc
    21301 cgatgcctgg ccggatgtgg tcgtgggctg caccggcggc gggtccaatt tcgccggcat
    21361 tgcctttccg ttcatcgggg acggcctgcg cggcggccag aagccgcgca tcgtggcggt
    21421 ggagccggcg gcctgcccgt cgctcacgcg cggcaaatac gcctacgatt tcggcgacac
    21481 cgggcacatg acgcccctga gcaagatgca caccttgggc agcgcgttca cgccgcccgg
    21541 tttccatgcg ggcgggctgc gctaccacgg gatggcgccg ctggtttcgc acctcaagga
    21601 gttgggcatg ctcgaagccg tggcctataa ccaggtcgag tgcttcgagg ccggcgtgct
    21661 gttcgcgcgc aacgagggaa tcgttcccgc gcccgaggcg aaccatgccg tcaagggcgc
    21721 gatcgaagag gcgctgcgct gcaagcggga gggcaaggcc gagacgatcc tgttcaacct
    21781 ctgcggccat ggccacttcg acatgctggc ctattccaaa tacttcgccg gtgagctgag
    21841 cgacgagaag tacgacgaga acgaactcgc gatggcgctc gccggcctgc cgagcgtgcc
    21901 agagccggcc tgagtcagcg gtaggcgacg gacagggctg tcatgcccct tgaggatgtc
    21961 atgcgctaag gctgtcatcc cggaccccgg atcaagtccg gggcaggccc gatccgggat
    22021 ccaggaaatc ctggattgcg gctccaaggc cgcaatgaca ggactccttt gacagcttcg
    22081 caatgacacg cacctacgcc ggctcggcca ccttgggctg cccgagcgaa cgcagcacgt
    22141 cgcgcaccat ctgcgcgcgg tccttcgggt ccttcagggc ccgctcgatg cgcagcttct
    22201 cgttgcccgc cag
//
LOCUS       DASWIY010000350         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3544681, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000350 DASWIY010000000
VERSION     DASWIY010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3544681"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1134
                     /locus_tag="VGA59_12570"
     CDS             118..1134
                     /locus_tag="VGA59_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9720556.1"
                     /translation="MNDLIRIGIDTSKSVFVLHGVDRSEQPVLRKKLRRRQVVEFFAA
                     LPPTRIGMEACGAAHHWGRELTALGHDVCLLPPQDVKPYVRRNKNDAADAEAICEAMS
                     RPRMRFVAVKSAEQQAALMLAGVRDQLVRRRTQVSNSIRGYAAEFGLVAAKGHDKIEP
                     LLARIAADETLPELAGELFAELGEDYGRLTLQLRKIDARLMAWHRQNELSRRLAEIPA
                     IGPVGASLLAMKVPNPAAFRSGRDFAAWIGLTPKDHSTAGKTRLGAITRAGDEHLRSL
                     LIVGATAVLQQVRRGRGYSSPWVSALIKRKPPKLAAVALANKTARIAWKLMVSGQHYN
                     HPRA"
BASE COUNT          281 a          485 c          542 g          276 t
ORIGIN      
        1 tcggctggcc cgggcacagg ctccagcggg aaaccagcgc tgttggggtg gacggccccc
       61 gacggcatcg atgtgccaga atgaggtcgt tgcagatctc atagaaagga gccgtccgtg
      121 aacgatctta tccgcattgg catcgacacg tccaagagcg tttttgtgct gcatggggtg
      181 gaccggagcg aacagccggt tttgcgcaag aagctgcggc ggcggcaggt ggttgagttt
      241 tttgcagctt tgccgccgac gcggatcgga atggaagcat gcggcgcggc gcaccattgg
      301 gggcgtgagc tgacggcctt ggggcacgac gtctgtctgc tgccgcccca agacgtgaag
      361 ccctatgtcc ggcgcaacaa gaacgatgcg gccgatgcgg aggccatctg cgaggcgatg
      421 agccggccgc ggatgcggtt tgtggctgtg aagtcggccg agcagcaggc ggcgctgatg
      481 cttgccggtg tgcgcgatca attggttcgt cggcgcacgc aggtgagcaa ttcgatccgc
      541 ggttatgcag ccgagttcgg actggttgcc gccaaggggc atgacaagat cgagccgctg
      601 ttggccagga ttgcggcaga cgagaccctt ccggagctgg ccggggagct gttcgcggaa
      661 ctgggcgagg actatgggcg gctcacgctc cagcttcgca agatcgacgc caggctgatg
      721 gcgtggcatc gacagaacga gttgagccgg cgtctggccg agattcccgc catcggtcca
      781 gtgggagctt cgctgctggc gatgaaggtt cccaatccgg ctgcgttccg ctccggccgc
      841 gacttcgcgg cctggatcgg gctgacgccg aaggatcatt caaccgccgg caagacgcgg
      901 ctcggcgcga ttacgcgcgc cggcgacgag catttgcgca gcttgttgat cgtcggcgcc
      961 accgccgtgc tccaacaagt gcggcgcggc cgcggatact cctcgccttg ggtcagcgcg
     1021 ctgatcaagc gcaagccgcc caagttggcc gcggtcgcgc tcgccaacaa gacggcgcgc
     1081 attgcctgga agctgatggt cagcggccag cactacaatc acccgcgcgc atgagactct
     1141 tgccggcgga gcagcctcgc tccgccggca aagccggaag cttgctgaga cggagcagat
     1201 ggcgtgatcg atcgatccca agacgcgaga cactccgtgg gacccagtgg ctcaactgaa
     1261 gtcgctgcgc tgttcggaac tcgcgtcgcg gaacccatct tggccagcgg tcgtgtgtgg
     1321 ccgcatcaac aggccggaca tatgatcgca agcgatccga tccgcacgct gaaacgtcct
     1381 tgcaagtcgg gggccgtcca catatgggtc cccgctttcg cggggacgaa cgggagagtg
     1441 cgtcgctggc tcgatgtttc aacctaaccg tatcttgccc tacaccctgt tgcccgcgct
     1501 ccacagccag aagtcgcggc cctcctcgcg caggaagcgg gagaacacca tcttgtgcac
     1561 ctcgtcggcg ccgtcgacca ggcg
//
LOCUS       DASWIY010000351         5812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_229436, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000351 DASWIY010000000
VERSION     DASWIY010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229436"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VGA59_12575"
     CDS             <1..819
                     /locus_tag="VGA59_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEX9720557.1"
                     /translation="PGLDALIDVLTPIALEGPVTGEAALAAMPPTRRAGSKPFAVEGF
                     NDITQQWEMPQAGQRYTAFQAGVQLANRTGALNEIEYSEFVVKVQAFADAINGSPEFP
                     EMLDEVARARELDQFASGHDAQLSFTLRARNAAWSPGYVQQNAARLGFVAGAIPGRMV
                     LPAEAAGGHPMLGLSFDSLAAMADDPAQTALRSLTLTLDVPQVDRGEQPFVRMRDAAM
                     ALAASMEGIITDDNGQVIRDEALDVIGADLEKLYDTLDARDLAAGSPQARRLFS"
     gene            850..2907
                     /gene="ligA"
                     /locus_tag="VGA59_12580"
     CDS             850..2907
                     /gene="ligA"
                     /locus_tag="VGA59_12580"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX9720558.1"
                     /translation="MPAPPKIQERVDALRELLHHHAHRYYVLDEPEIPDAEYDRLFRE
                     LQELEDRYPELLTPDSPTQRVGGKLLEGFAKVRHRVPMLSIRTETDIEASGARSFDAR
                     VRRELGLAESDPPIEYVAELKFDGLAISLRYENGALVQAATRGDGEVGEDVTQNIRTI
                     GQIPLRLPKDAPAVLEVRGEVYIRRDDFERLNELQREKIARGAKGEKTFVNPRNAAAG
                     AVRQLDPHIARQRPLSFFAYGWGEVTPPQQGGPAFETQFEAMLTLRSWGFPVSVRTQL
                     ATGAQELIAYHQSVGKERDSLPFDIDGVVYKVNSLALQRRLGFVTREPRWAVAHKYPA
                     QEQMTTVEGIDVYVGRTGKLTPVARLAPVFVGGVTVTNATLHNEDEARRKDVRVGDTV
                     IVRRAGDVIPEVVAVVPEKRVHHAPLFTMPRRCPVCGSEAVREEGEVDYRCTGGLFCG
                     AQRKQALLHFAQRRALDIEGLGEKLVDQIVDGNIIKTLPDLYRLGLNALAGLERMAEK
                     SAQNLLAALEHSKRTTLPRFLFGLGIRHVGEATAKDLARHFGSIDAIMDASVEQLLEV
                     ADVGPVVAESIHTFFQQPHNREVVEQLRACGVTWDEGEPAARAPKPLAGKTIVLTGTL
                     PTLSRDEAKDLLEAAGAKVAGSVSKKTDYVIAGAEAGSKLDKALELGVKVLDEDGLRS
                     LLG"
     gene            complement(2950..3372)
                     /locus_tag="VGA59_12585"
     CDS             complement(2950..3372)
                     /locus_tag="VGA59_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEX9720559.1"
                     /translation="MTSRPPGDPHPSALRPLSRPVRWLLKGVAVMSLALGIIGAFVPV
                     LPTVPFILLAAWAAARSSPRLSHWLENHPRMGPHIRDWRNGGVVSRPAKWMATAMMAS
                     GGAIMMIVVRPLWVPLAVIAIMTTVGVWLWRRPEQAPS"
     gene            3422..3961
                     /gene="def"
                     /locus_tag="VGA59_12590"
     CDS             3422..3961
                     /gene="def"
                     /locus_tag="VGA59_12590"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX9720560.1"
                     /translation="MAVREILKMGDPRLLRVAQPVTAFDTDEIHLLVSDMFDTMRAAD
                     GAGLAAPQIGVDLQLVIFGTEAVNPRYPDAPLVPRTVLLNPVITPIGTQEEDGWEGCL
                     SVPGLRGVVPRWARIRYTGFDQYGDAIDRTVEGFHARVVQHECDHLIGKLYPMRVRDF
                     TRFGFTDILFPELDAGQDD"
     gene            complement(3990..>5812)
                     /locus_tag="VGA59_12595"
     CDS             complement(3990..>5812)
                     /locus_tag="VGA59_12595"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX9720561.1"
                     /translation="REDRLVFDLQTAVAESFGYRTQTQPGGRVISRASEALMKRYYWA
                     AKAVTQLNQILLLNIEERLNPKQHEPQPINERFSIKAGMIEVASDDLYLKHPQAILET
                     FLVHQTTVGAEGLSARTLRALYNARKVMDANFRNDPANHATFRAILMAHQGITHAMRL
                     MNQTSVLGRYLWAFRRIVGQMQHDLFHVYTVDQHILMVLRNVRRFFIAEHAHEYPFCS
                     QLAAGWDKPYILYVAALFHDIAKGRGGDHSELGARDVRQFCRQHCIDPEDCKLIAFLV
                     REHLAMSRIAQKQDLSNPDVIAAFARRVGNERYLTALYLLTVADIRGTSPKVWNAWKG
                     KLLEDLYRLTLRTLGGRAPDPDAEVEARKREALVQLALYALPFEAHKKLWDTLDVGYF
                     MRHDAGDIAWHTRHLSRHVGKAKAIVRARLSPVGEGLQIVVYTPDQPDLFARICGYFD
                     QAGFSILDAKIHTAKNGYALDTFQVVSAMLPEHNRELISMVQAELQHTIDRAGPLPAP
                     SRGRVSRRVKSFPIAPRVHLAPDEKAQRWLLSISASDRVGLLYSVARVLARHKINLQL
                     AKVSTLGERVEDTFLIDGAELQQNRRQIEIETELLETLAA"
BASE COUNT          984 a         1914 c         1998 g          916 t
ORIGIN      
        1 cccggcctgg acgcgctgat cgacgtgctg acgcccattg cgctggaagg gcccgtcacg
       61 ggggaggccg cgctggcggc catgcccccg acccgccgcg ccggcagcaa gccgttcgcc
      121 gtggaaggct tcaacgacat cacgcagcaa tgggaaatgc cgcaggcggg gcagcgctac
      181 accgcattcc aggcgggggt acagctggcc aatcgcaccg gtgcgctcaa cgagatcgaa
      241 tattccgagt tcgtcgtgaa ggtgcaggcg ttcgccgacg cgatcaacgg ctcgcccgag
      301 tttccggaga tgctcgacga agtggcgcgg gcccgtgaac tcgaccagtt cgccagcggc
      361 cacgacgcgc agctgagctt cacgctgcgc gccaggaacg ccgcctggag tcccggctac
      421 gtgcaacaga acgcggcgcg cctcgggttc gtcgcgggcg ccattcctgg acggatggtg
      481 ttgccggccg aagcggccgg cgggcacccg atgctgggcc tgtcgttcga ttcgctggcc
      541 gcgatggccg acgacccggc ccagacggcc ctgcgttcgc tgacgctcac cctggacgtg
      601 ccccaggtgg accgcggtga gcagcccttc gtgcgcatgc gcgacgccgc gatggcgctg
      661 gcggcttcga tggagggcat catcaccgac gacaacggcc aggtcatccg cgacgaggcg
      721 ctggacgtga tcggcgccga cctcgagaag ctgtacgaca cgcttgacgc gcgcgatctg
      781 gcagcgggct caccccaggc gcggcgcctg ttctcgtagg ccgggcagca cgctgccgcc
      841 atcctcgcca tgcccgcccc tccgaaaatc caggaacgcg tcgacgcact gcgcgaactg
      901 ctgcatcacc acgcgcatcg ctactacgtg ctggacgagc cggaaatccc ggatgcggaa
      961 tacgacaggc tgtttcgcga actccaggag ctggaggacc ggtatcccga actcctgacg
     1021 ccggactcgc ccacgcagcg cgtgggcggc aagctgctgg aaggcttcgc caaggtgcgc
     1081 cacagggtgc cgatgctgtc catccgcacc gagaccgaca tcgaggccag tggcgcgcgc
     1141 agcttcgacg cgcgcgtgcg gcgcgagctg ggactggccg aatccgatcc gccgatcgaa
     1201 tacgtggccg agttgaagtt cgacgggttg gcaatcagcc tgcgctacga aaacggtgcg
     1261 ctggtccagg ccgccactcg cggcgacggc gaagtgggcg aggacgtgac gcaaaacatc
     1321 cgcactatcg ggcagattcc cctgcgtttg cccaaggacg cacccgccgt gctcgaagtg
     1381 cgcggcgagg tctacatccg gcgcgacgac ttcgaaaggc tcaacgagct gcagcgcgag
     1441 aagatcgccc ggggcgccaa gggcgaaaag acattcgtga atccccgcaa tgccgccgcc
     1501 ggcgcagtgc gccagctcga cccccacatc gcgcggcagc ggccgctgag cttcttcgct
     1561 tacggctggg gcgaggtgac accgccgcag cagggggggc cggcattcga gacgcagttc
     1621 gaagccatgc tgaccctgcg ctcctggggc tttccagtgt ctgtgcgcac ccagctggcc
     1681 accggggcgc aggaactgat cgcctaccac cagtccgtcg gcaaggagcg cgacagcctg
     1741 cccttcgaca tcgacggcgt ggtctacaag gtcaacagcc tggcgctgca gcggcggctg
     1801 ggcttcgtga cgcgcgagcc gcgctgggcc gtggcgcaca agtacccggc gcaggagcag
     1861 atgacgaccg tcgaagggat cgatgtgtac gtcggacgca ccggcaaact gacacccgtg
     1921 gcgcggctcg cgccggtgtt cgtcggaggc gtcaccgtca ccaacgccac gctgcacaac
     1981 gaggacgagg cccggcgcaa ggacgtgcgg gtgggcgaca ccgtcatcgt ccggcgcgcc
     2041 ggtgacgtca ttcccgaggt ggtcgccgtg gtgcccgaaa agcgcgtgca ccacgcaccg
     2101 cttttcacca tgccgaggcg ctgtccggtc tgcggctccg aggccgtgcg ggaggagggc
     2161 gaggtcgact accgctgtac cgggggcctg ttctgcggcg cccagcgcaa gcaggccctg
     2221 ctgcattttg cccagcggcg ggcgctggac atcgagggcc tgggtgaaaa actggtcgac
     2281 cagatcgtgg acggcaacat catcaagacc ttgcccgacc tgtaccgcct gggcctgaac
     2341 gcgctggccg gcctggaacg catggcggaa aaatcggccc agaacctgct ggcggcgctg
     2401 gagcattcca agcgcaccac cttgccgcgc ttcctgttcg gcctgggcat ccgccatgtc
     2461 ggcgaagcca cggccaagga cctggcgcgc catttcggca gtatcgacgc catcatggat
     2521 gccagcgtgg agcagctgct ggaagtcgcc gacgtgggtc cggtggttgc cgaaagcatc
     2581 cacacgttct tccagcagcc ccataaccgc gaggtggtgg aacagctgcg ggcctgcggc
     2641 gtcacctggg atgagggcga gccggcggca cgcgcgccca agcccctggc gggcaagacc
     2701 atcgtgctga cgggcacgct gcccacgctg agccgtgatg aagccaagga tttgctggag
     2761 gcggccggcg ccaaggtggc gggttcggtc agcaagaaga ccgactatgt catcgcgggc
     2821 gcggaagccg gcagcaaact cgacaaggcg ctcgagctgg gcgtgaaggt actcgacgag
     2881 gacgggctgc gctcgctgct gggctgagcg cgcatggatt cgaacggcga gagcacggat
     2941 ttcgttggac taggaaggcg cctgttccgg acgccgccag agccacacgc ccacggtggt
     3001 catgatcgcg atcaccgcca agggcaccca aagcggccgc acgacgatca tcatgatggc
     3061 cccaccggaa gccatcatgg ccgtggccat ccacttggcc ggccggctca ccacgccgcc
     3121 gttgcgccag tcgcggatgt gcggtcccat gcgcggatgg ttctcaagcc agtgcgacag
     3181 gcgcggcgag ctgcgcgccg ctgcccaggc ggccagcagg ataaaaggca ccgtcggcaa
     3241 gacgggcacg aaggcgccga tgatgccaag cgccaggctc atgacagcga cccccttgag
     3301 cagccagcgc acgggccggg acagcggccg cagcgcggac gggtgcggat cacctggagg
     3361 tcgtgaggtc atgaacggat tgtgccggta ggagcccagg ctcccacgct atgcttcacc
     3421 catggctgtc cgtgaaatcc tcaagatggg cgacccgcgg ctgttgcggg ttgcccagcc
     3481 cgtgaccgcg ttcgacaccg acgagattca cctgctggtg tccgacatgt tcgacaccat
     3541 gcgcgccgcc gatggcgcgg gcctggccgc gccccagatc ggggtggacc tgcagttggt
     3601 gatcttcggc accgaagccg tgaacccgcg ctaccccgat gcgccgctgg tgccgcgcac
     3661 ggtgctgttg aatccggtca tcacgcccat cggcacgcag gaagaagacg ggtgggaagg
     3721 ttgcctctcg gttccgggcc tgcggggtgt ggtgccgcgc tgggccagga tccggtatac
     3781 cggcttcgat caatatggcg acgcgatcga ccgcaccgtg gaaggctttc acgcgcgcgt
     3841 ggtgcagcac gaatgcgatc acctgatcgg caagctttac ccgatgcggg tgcgcgactt
     3901 cacccggttc gggttcaccg acattctgtt ccccgagctc gatgcgggac aggacgacta
     3961 gggcctgttc acactctccc tgcgtcgaat caggccgcca gcgtttccag caactccgtc
     4021 tcgatctcga tctgccggcg gttttgctgc agctcggcgc cgtcgatcag gaaggtgtct
     4081 tcgacccgct cgccgagcgt ggaaaccttc gccagctgca ggttgatctt gtgccgtgcc
     4141 agcacacgcg ccaccgaata aagcagcccg acgcggtcgc tggcggaaat gctcagcagc
     4201 cagcgctggg ctttctcgtc gggcgcgagg tgcacgcgcg gcgcgatcgg gaagctcttg
     4261 acccggcgcg acacccggcc ccggctgggg gcggggagcg ggccggccct atcgatcgtg
     4321 tgctgcagct ccgcctggac catgctgatc aattcgcggt tgtgctcggg cagcatggcg
     4381 ctgacgacct ggaaggtatc gagcgcatag ccgttcttgg cggtgtggat cttggcgtcc
     4441 aggatgctga agccggcctg gtcgaagtag ccgcagatgc gggcgaacag gtcgggctgg
     4501 tccggcgtgt acaccacgat ctgcaggcct tcgcccaccg gagacagccg cgccctgacg
     4561 atggccttgg ccttgcccac atggcgcgac aggtggcgcg tgtgccaggc gatgtcgccg
     4621 gcgtcatggc gcatgaaata ccccacgtcc agcgtgtccc acagcttctt gtgggcctcg
     4681 aacggcagcg cgtacagcgc cagctggacc agcgcctcgc gcttgcgcgc ctccacttcg
     4741 gcgtccgggt cgggcgcgcg cccgcccagg gtgcgcagcg tgagtcggta aaggtcctcc
     4801 agcagcttgc ccttccaggc attccagacc ttggggctgg tgccgcggat gtcggccacc
     4861 gtgagcagat acaacgccgt gaggtagcgc tcgttgccaa cgcgccgggc gaaggcagcg
     4921 atgacgtccg ggttgctcag gtcctgtttc tgcgcgatcc ggctcatggc caggtgttcc
     4981 ctgaccagga acgcgatcag cttgcaatcc tcggggtcga tgcagtgctg gcggcaaaac
     5041 tggcgcacgt cgcgcgcccc cagctcggaa tggtcgccgc cacgcccctt ggcgatgtcg
     5101 tggaacagcg ccgccacgta gaggatatag ggcttgtccc agccggccgc cagctgggag
     5161 cagaaggggt actcgtgcgc gtgctcggcg atgaagaagc ggcgcacgtt gcgcagcacc
     5221 atcaggatgt gctggtccac cgtgtacaca tgaaacaggt catgctgcat ctgaccgacg
     5281 atgcggcgga acgcccagag gtagcggccc agcaccgagg tctggttcat cagccgcatc
     5341 gcgtgcgtga tgccctggtg cgccatcaga atcgcccgga aagtggcatg gttcgcggga
     5401 tcgttgcgga agttcgcgtc catcaccttg cgggcgttgt acagcgcgcg cagcgttcgc
     5461 gccgacaagc cctcggcgcc cacggtggtc tggtgaacca ggaacgtctc caggatggcc
     5521 tgcggatgtt tcaggtacag gtcgtcgctg gcgacctcga tcattccggc cttgatggaa
     5581 aagcgctcgt tgatgggctg cggctcatgc tgtttcgggt tcagccgctc ctcgatgttg
     5641 agcagcagga tctggttgag ctgggtgacg gccttggcgg cccagtaata gcgcttcatc
     5701 agcgcctcgc tcgcgcggga gatcacgcgt ccgcccggct gggtctgggt ccggtagccg
     5761 aaggactcgg ccacggcggt ctgcaagtcg aacaccaggc ggtcttcgcg cc
//
LOCUS       DASWIY010000352         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1727201, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000352 DASWIY010000000
VERSION     DASWIY010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727201"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /gene="mupP"
                     /locus_tag="VGA59_12600"
     CDS             complement(<1..596)
                     /gene="mupP"
                     /locus_tag="VGA59_12600"
                     /inference="COORDINATES: protein motif:HMM:NF009696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate phosphatase
                     MupP"
                     /protein_id="HEX9720562.1"
                     /translation="MTRPMRLRAVLFDMDGTLLDSAPDFIAICQAMRAERGLAAVDDQ
                     LIRDQISGGARAMVAASFAMDPLAEQFEALRLEFLERYQDHCALLTRPFDGIQELLAD
                     IEQAKLIWGVVTNKPLRFAEPIMQQLGLAGRSAILICPDHVSNSKPDPEPMILACSQL
                     GLDPASVLFVGDDLRDIESGRAAGTRTAAVTYGYIHPD"
     gene            complement(734..1432)
                     /gene="ubiG"
                     /locus_tag="VGA59_12605"
     CDS             complement(734..1432)
                     /gene="ubiG"
                     /locus_tag="VGA59_12605"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005261499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX9720563.1"
                     /translation="MSNVDHAEIAKFEALAHRWWDRESEFKPLHDINPLRVNWIEERV
                     SLAGQKVLDVGCGGGILSEAMAQRGAEVTGIDMGEAPLAVAQLHQLESGVSVEYRQIT
                     AEALAAEMPEQFDVVTCLEMLEHVPDPASVIRACHSLVKPGGQVFFSTLTRNPTAYMF
                     AVIGAEYLLRLLPRGTHDFKKFIRPSELGAWSREAGLQVKDIIGLTYNPLTKHYKLQA
                     DVDVNYMIQTLKEG"
BASE COUNT          288 a          475 c          556 g          333 t
ORIGIN      
        1 tcgtccgggt gaatatagcc ataggtcacc gcggcggttc tggtgccggc ggcgcgtccc
       61 gactcgatgt cgcgcaggtc atcgccgacg aacaggacgc tggccgggtc caggcccaac
      121 tggctgcagg caaggatcat cggctctggg tcgggtttgc tgttgctcac atgatccggg
      181 cagatcagga tggcggagcg cccggccagg cccagttgct gcatgatcgg ttcggcgaag
      241 cgcagcggct tgttggtcac cacgccccag attagtttgg cctgctcgat atcggcgagc
      301 agttcctgga tgccgtcgaa agggcgggtc agcagcgcac agtgatcctg atagcgctcg
      361 agaaactcca ggcgcagggc ctcgaactgc tcggccagcg gatccatggc gaagctcgcg
      421 gcaaccatgg cgcgggcgcc gccggaaatc tgatcgcgga tcagctggtc atccacggcc
      481 gccaagccgc gttcggcacg catcgcctgg cagatcgcaa tgaagtccgg ggcactgtcg
      541 agcagggtgc catccatatc gaaaagaact gcgcgcaaac gcatcgggcg tgtcattggc
      601 tatttccctc gctgcgccgg agtctgtttt ggcgcgaggc aaggcacgag ccgtgaagtc
      661 tggttgttcc aaatgagcgg cgagaaacgc cgcatcgcgc caaaacagac ccgtaaataa
      721 ccaatggcac gccctagccc tccttgagcg tctggatcat gtaattgacg tcgacatccg
      781 cttgcagttt gtagtgtttg gtcagcgggt tgtaggtcag gccgatgatg tccttgactt
      841 gcaggccggc ttcgcggctc caggcgccca gttccgaggg gcggatgaac ttcttgaagt
      901 catgggtgcc gcgcggcagc aggcgcagca ggtactctgc gcctatcacc gcgaacatat
      961 aggcagtggg gttgcgggtg agggtggaga agaacacctg gccgccgggt ttgaccaggc
     1021 tgtggcaggc gcggatcacc gaggccggat cggggacgtg ttcgagcatt tccaggcagg
     1081 tgaccacgtc gaactgctcg ggcatttccg cggccagggc ttcggcggtg atctgccggt
     1141 actcgaccga gacgccggat tccagttggt gcaactgggc cacggccagc ggcgcttcgc
     1201 ccatgtcgat gccggttact tcggcgccgc gctgggccat ggcttcgctg aggatgccgc
     1261 cgccgcagcc gacgtcgagt accttctggc cggccaggct gacgcgttct tcaatccagt
     1321 tgacccgcag tggattgatg tcgtgcaggg gtttgaattc gctttcgcgg tcccaccagc
     1381 ggtgggcgag ggcttcgaat ttggcgattt cggcgtgatc gacgttgctc atgatgttcc
     1441 ctggatagaa ctggaggcag gaagcctgac gcttgaagtg cctatggtgc cagtttcacg
     1501 ttgtggggtg gacgcctgcg ggcgaatcaa tgtatgtcag tttgtcgcag tctgtaggag
     1561 ccagctcgct ggcgatctgg catcccggtg ctgccggtga tcgccagcaa ggactaggcg
     1621 tccccctggc tcctacctgt tatgtataca tg
//
LOCUS       DASWIY010000353         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_608772, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000353 DASWIY010000000
VERSION     DASWIY010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608772"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..920
                     /locus_tag="VGA59_12610"
                     /pseudo
     CDS             1..920
                     /locus_tag="VGA59_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612008.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1016..1757
                     /locus_tag="VGA59_12615"
                     /pseudo
     CDS             1016..1757
                     /locus_tag="VGA59_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611730.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          391 a          647 c          625 g          339 t
ORIGIN      
        1 ccgctgcacg cgaagatcgg gcaactggcg ctggagaatg attttttgga aggcgcgctc
       61 accaaagcgg gattgctgag cgcaaagcca tgatcgtccg agaccacgaa ttgagcatca
      121 cgcgccaggc ccagttgttg gagatcagcc gtgggacggt gtattacctg ccccggccgg
      181 tcagcccagc cgatctggcg ctgatgcgca gactcgacga gttgcacctg caacacccct
      241 tcatgggggc gcgcatgctg cgcgaccagt tggcccggca gggcatcccg gcggggcggc
      301 gccacatccg aacgctcatg ctgcgcatgg gtatcgaggc cctggcgccg cagccgggca
      361 ccagccaagc cgcgcctggg cacaaggtct acccctacct gctgcgcgcg ctggccattg
      421 cccgcgccaa ccaggtctgg gcgctggaca ccacctacat tcccatggcg cggggctttg
      481 tctacctcac cgccgtggtg gacgtggcct cgcgccgggt actcgcgcac aaggtggcga
      541 tcacgctgga agcggttcac gccagggaag tcatcgagca ggcctttgcc cggtacggtg
      601 tgcccgagat cgtcaatacg gaccaaggca gccagttcac ggcggtggaa ttcaccgatg
      661 tcgtgctggc tcagggctgt aagctctcca tggacgggcg cggcgcctgg cgtgacaacg
      721 tctttgtgga gcgcctgtgg cgcagcgtga aatacgaacg ggtctacctc aaggcctacg
      781 acagcgtgag cgcggccaga gcggacatcg ccgactacct ggactggtac aacgcgcatc
      841 gcagtcattc aagtctcgaa cggctcacgc caaacgaaaa gtactgggcc gccttgccgc
      901 agttgaagcg ggccgcgtaa catgaaaacc ttggtgcacc ccgactcacc attcgcaacc
      961 caccctgagt ccacttataa atcagggaaa gttgttcaaa caaacggagc cacttctcag
     1021 cccggccgat ctggcgctga tgcgcagact cgacgaactg cacctggaac accccttcat
     1081 gggggcgcgc atgctgcgcg accaatttgg cccggcaagg tgtccatgcc ggacggcgtc
     1141 atatcagaac gctcatgctg cgcatgggta tcgaggcctt ggcgccgcag ccgggcacca
     1201 gccaagccgc gcctgggcac aaggtctacc catacctgct gcgcgcgctg gccattgccc
     1261 gcgccaacca ggtctgggcg ctggacacca cctacattcc catggcgcgg ggctttgtct
     1321 acctcaccgc cgtggtggat gtggcgagcc gcaaggtgtt ggcgcacaag ctggcgatca
     1381 cgctggaagc ggttcacgcc aaggaggtga tcgagcaggc cttcgcccgc tacggggcgc
     1441 ccgagatcgt caacaccgac cagggcagcc agttcacggc cgaggagttc acccgcgtcg
     1501 tgctggccaa gggctgcaag ctgtccatgg acgggcgtgg cgcctggcgc gacaacgtgt
     1561 tcgttgagcg cctgtggcgc agcgtgaagt acgaacgggt gtacctcaag gcctacgaca
     1621 gcgtgagcgc ggcacgcatc gacattgccg actatctgga ctggtacaac gcgcaccgca
     1681 gtcattcaag tctcgaacgg ctcacgccga acgaaaagta cctcgcagca ctgccgccga
     1741 tggcgctggc cgcgtaagat gaaatcctgg gtgcgccccg agttgcccac cgcgtcgatc
     1801 ggttcgtcgc aagcgccgag cgccgccgtg gacaactctg caccgttcgc aactcgcccg
     1861 gagtccactt ataaatcggg gaaaactgtt caaacaagcg gagccacttc tcttcaatgt
     1921 cggcggcgga ggttatcggg tggtggtcgc gattcatttt aaccggcaga agctctatat
     1981 ccggcacgta ttcacgcacg cc
//
LOCUS       DASWIY010000354         4652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2099806, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000354 DASWIY010000000
VERSION     DASWIY010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099806"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..898
                     /locus_tag="VGA59_12620"
     CDS             77..898
                     /locus_tag="VGA59_12620"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX9720564.1"
                     /translation="MIDLAPSHKRGLALKSPVLNAAGTLGFADEYAGLIDFSLLGAFV
                     TNPITARPRTPARGEHARQFDGGVLIHTGLPNPGIGEAVRHHARRWARLACPVVVHLA
                     ATMPEEVAAGIERLEALENVAGVEIGLRDDVEAAEAEALLAAAARLASLPVLARLPLL
                     AAEKLAGAAARAGVQAVVVGGPPRGALPTPEGGWLAGRVYGPAVLPLALQAVRAVAGR
                     LDLPVIGAGGIHRAEDAQAMLQAGAVAVQIDSAAWRDPRVFERTAASLGAGAPAQ"
     gene            complement(906..2672)
                     /locus_tag="VGA59_12625"
     CDS             complement(906..2672)
                     /locus_tag="VGA59_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720565.1"
                     /translation="MDRLPLDPNSQTFVETIGPDVGMHPDFGSGLYEGEPIGIPYVEV
                     PADQAGVTVAFEYDDESDPGPYPIPADPPIEGGPNSDNDRHILIVQRDACLLFELYYA
                     WPENDGTWRADSGAIFDLNSNQLRPETWTSADAAGLPILPGLVRYEEVSAGEIRHALR
                     FTAPETQHAYVWPARHFASELTGQAYPPLGQRFRLRGDFDLSSFSPDVQIILRALQRY
                     GMFLADNGSAWFLSGAPDERWDNDVLSELGQVQGSDFEAVDASSLMVDPDSAQAAGPT
                     FEAGPLPPTSAPGAAGQGGHISYTLENNRVYLIAAEAGAQPEDVSQALDGLAEGADDG
                     WLNLSPDGSWLALDTDRFDSECVGWTCLAILPADLSSGEAIRAGDELVHPEGKSAVAS
                     GGNLLVKPAGGGPHDRDLFVSTRSGGEWSATAALTEASPYAWNDLPAISDDGARIVFE
                     CGPQPYGAAGTAICEVGTDGSGFRVVLAPEQGPGGSAGNALRHPDYAPDGSLVFEADW
                     SGQQIWRLPAGGGQPALVSPGFSNDNSPCVLPDGRIVSLWLERPGGNGLHEIKVMPPD
                     GSSYEMILTGVDVHDVGIGCGA"
     gene            3164..3499
                     /locus_tag="VGA59_12630"
     CDS             3164..3499
                     /locus_tag="VGA59_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720566.1"
                     /translation="MSKSKSRRHLHHCKCAACEAHPYGRVARQHQAINRVMATFDEKG
                     RRRFAGLLALQFGRGGVQLAHAITGLSRMTIRAGREEIQRTDRTPGVRRPGAGRPAVE
                     KSNPGSWRR"
     gene            3487..4443
                     /locus_tag="VGA59_12635"
     CDS             3487..4443
                     /locus_tag="VGA59_12635"
                     /inference="COORDINATES: protein motif:HMM:NF033519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEX9720567.1"
                     /translation="MEALSELLKDATAGDPIRGTKWTRKTLRKLVQELRHQKFKVGHE
                     TVRRLLKQLNYWLRYNRKERSKRQDVDRDRQMHYLARKRRAFLKAKKPVISVDTKKKE
                     LIGNFKNPGRTWRQEARSVLATDFPSDADGKAIPYGIYEVAGNRGFVGVGMSHETAEF
                     AVNVIRRWWQAEGRAVSPRASELLIQADGGGANGYRSWLGKWELQRLADETGLTITVT
                     HYPTSASKWNWIEHRLFNHISANWAGEPLVNYHTVLNFIRTTTTETGLCCTAYLDRKH
                     YSTKLTLTPEQTKAIRLTKHRVLPKWNYTIRPHPPSGGKTAK"
BASE COUNT          797 a         1517 c         1539 g          799 t
ORIGIN      
        1 tttaccccga aggcgcgaag atcgcaaagg acgctaagtt agcatccttt gcgcctttgc
       61 ggttcagttt tgatccttga ttgacctcgc cccctcccac aagcgcggcc tggcgctcaa
      121 gtcgccggtg ctcaacgccg ccggcacgct cggctttgcc gacgaatacg ccgggctgat
      181 tgacttctcg ctgctcggcg cgttcgtcac caaccccatc acggcccggc cgcgcacacc
      241 ggcccgaggc gagcatgcgc gccagttcga cggcggcgtg ctgatccaca ccggcctgcc
      301 caaccccggc atcggcgagg ccgtgcgcca tcatgcgcga cgctgggcgc ggctggcctg
      361 cccggtcgtc gtccatctcg ctgctaccat gcccgaagag gtagcagccg gcatcgagcg
      421 tttggaagcc ctggagaatg tggccggggt agagatcggg ttgcgtgacg atgtggaagc
      481 ggcggaggcc gaggcattgc tggccgccgc cgcgcgcctg gcaagcctgc ccgttctggc
      541 ccgcttgcct ctgctcgcgg ccgagaagtt agccggggcc gccgcccgcg ctggggtgca
      601 ggcggtggtg gtgggcggcc cgccacgcgg cgcgttgccc acgcccgagg gcggttggct
      661 ggctgggcgc gtctatggcc cggcggtgtt gccgctggcg cttcaggcag tcagggccgt
      721 cgcgggccgc ctcgatttgc cggtcatcgg cgcgggcggc attcatcggg ccgaggacgc
      781 tcaggccatg ttgcaagccg gggccgtcgc ggtgcagatc gattcggccg cctggcgcga
      841 cccgcgcgtg ttcgagcgca ccgccgccag ccttggggcg ggcgctccgg cccaataagg
      901 cggctctagg cgccgcagcc gatgcctacg tcatgcacgt ccacgccggt caggatcatc
      961 tcgtaactgc tcccgtcggg gggcatgact ttgatctcgt gcaggccatt cccgcccggc
     1021 cgctccagcc acagggaaac aatgcggccg tccggcagaa cgcacggcga attgtcgttg
     1081 gagaaacctg ggcttaccag cgccggttgg ccgccaccgg ccggcagtcg ccaaatctgc
     1141 tggccgctcc aatcggcctc aaatacgagg ctgccgtcgg gagcgtaatc gggatggcgc
     1201 agcgcgttcc cagccgagcc gcccggcccc tgctcgggcg caagcaccac ccggaagccg
     1261 ctgccgtcgg tgcctacctc gcagatagcc gtgcccgccg cgccgtacgg ctgtgggccg
     1321 cattcgaaca caatccgcgc gccgtcatct gaaatggcgg gcaagtcgtt ccaggcatag
     1381 ggcgaggcct cggtcagcgc ggcggttgcg ctccattctc cgccgctgcg cgtggagaca
     1441 aacaagtccc gatcgtgcgg accgcccccg gccggtttga caagcagatt cccgccggag
     1501 gccacggcgc tcttgccttc gggatgcacc aattcgtctc cggcgcgtat cgcttcaccc
     1561 gatgacagat cggccggcag gatcgccagg cacgtccagc ccacgcactc gctgtcgaag
     1621 cggtctgtgt ccagcgccag ccacgatcca tcgggcgaaa ggtttagcca gccgtcatcg
     1681 gcgccctcgg ccagcccgtc cagcgcctgg ctcacgtcct ccggctgcgc gcccgcctcg
     1741 gcggcgatga gatacacccg gttgttttcg agcgtatagc tgatatgccc gccctggccg
     1801 gctgcgcctg gggcgctggt gggcggcagc gggccggcct cgaacgtggg cccggccgcc
     1861 tgagccgagt cgggatccac catcaacgag gacgcgtcta ctgcttcgaa atctgatccc
     1921 tgcacctgcc ccagttcact cagcacgtca ttgtcccaac gctcgtccgg cgcgccgctg
     1981 aggaaccagg ccgaaccgtt gtcggccagg aacatgccgt agcgctggag ggcgcgcagg
     2041 ataatctgaa catcgggcga gaagctggac aagtcgaagt caccccgcag gcggaagcgc
     2101 tggcccagcg gcgggtatgc ctgcccggtc agctccgagg cgaagtgccg ggccggccac
     2161 acatacgcgt gctgcgtctc gggcgcggtg aagcgcaggg cgtgccggat ctcgccggcc
     2221 gacacctctt catagcgcac caggcccggc aggatgggca ggccggctgc gtcggccgag
     2281 gtccaggtct cgggccgcag ttggttggag ttcaggtcga agattgcccc cgaatcggct
     2341 ctccacgttc cgtcattctc cggccaggcg tagtaaagtt caaataagag gcaggcgtcg
     2401 cgctggacga tcagaatgtg ccggtcgttg tcgctgttgg ggccgccctc gataggcggg
     2461 tcggccggga tggggtacgg gcccgggtcg ctctcgtcat cgtattcaaa ggctactgtc
     2521 acgccagcct ggtcggcggg cacctccacg tagggaatgc caatcggttc gccctcgtac
     2581 agcccggatc caaaatcagg gtgcatgccc acgtcgggcc cgatggtctc gacgaacgtc
     2641 tgtgaattgg ggtctagcgg cagcctgtcc acgggcacgt tccacacgtt gtcgcccgga
     2701 aacaggggac agttggccag cgacgggccg ggcccggccg gcgcagcggc gggcggttgc
     2761 gtggcggctg gaggggcttc aaccgagggc gcttcaactg ccggggcctc ggtgggcggc
     2821 aggagaggcg gggcggtggg aggtggttgg gagagcgccc ccaggctgca cgccagagcg
     2881 gccagcgcgg cgccggaaag gctggcgaga cgccgcagtc tgcggctatt cgtcgtcatc
     2941 gcagtatccc ctcgtgggcc gacgatagtt cagaacggac ttactctcta ttataggccc
     3001 aacccgattc cttgccgggg ccttgcccgc gcggcctcga gcgccaagat gcgaccgtcc
     3061 cgcgccgaac taaggatttg tccagaaata agttgtccgt tttgatgtaa actcaggctc
     3121 cgaccgcacg ccgggaacac cggcctgggt tagggcctga gctatgagca aatcgaaatc
     3181 gcgtcgtcac cttcatcatt gcaagtgtgc tgcctgtgaa gcgcatcctt atggtcgtgt
     3241 ggctcggcag catcaggcca tcaatcgggt gatggcgacc tttgatgaga aaggccgccg
     3301 ccgctttgcc ggactgctcg cactccaatt cgggcgcggc ggcgtgcagt tggcccacgc
     3361 gatcaccggt ctcagccgca tgaccatccg cgccggtcgc gaagaaatcc agcgcactga
     3421 ccgcacgccg ggcgtgcgcc gcccgggtgc tggtcgtccg gcggttgaaa aaagcaaccc
     3481 aggatcttgg aggcgctgag cgagttgttg aaggatgcca cggcgggtga cccgatccgc
     3541 ggcacgaagt ggacgcgcaa gacgctgcgc aagctggtcc aagaactgcg gcaccagaag
     3601 ttcaaggtcg gtcatgaaac ggtccgacgc ctgctgaagc aactgaatta ctggctgcgt
     3661 tacaaccgca aggagcgcag caaacgtcag gacgtcgacc gagaccggca gatgcactac
     3721 ctggcccgca agcggcgcgc ctttctgaag gccaaaaagc cagtgattag cgtggatacc
     3781 aagaagaagg agttgattgg caacttcaag aaccccgggc gtacctggcg ccaggaagcg
     3841 cgctcggtgc tggccaccga ctttcccagc gatgccgacg gcaaggctat tccgtatggg
     3901 atttacgagg tggctggcaa tcgtggcttc gtcggggtgg gcatgtcgca tgagacggct
     3961 gagtttgcgg tcaatgtcat tcggcgttgg tggcaggccg aaggccgggc ggtctctccc
     4021 agagcaagcg agttgctgat ccaagcggac ggcggtggcg ccaatggcta tcgcagttgg
     4081 ttggggaaat gggagttgca gcgcctggca gacgaaaccg gcctgacgat caccgtcacg
     4141 cactatccga ccagcgcctc gaagtggaac tggatcgagc atcggctctt caatcatatc
     4201 agcgccaact gggccggcga acccttggtc aattaccata cagtcttgaa cttcattcgg
     4261 acgaccacta ctgaaacggg cctgtgctgc acagcgtatt tggatcggaa acactactcg
     4321 accaagctca ctttgacacc cgaacagacg aaggccatcc gcctgaccaa gcaccgcgtg
     4381 ctgccgaaat ggaactacac catccggccg caccctccat cgggcggaaa aacggccaag
     4441 taattcttgg acaaatcctt agtgtcttcg tgtcttcgtg gctaaaagca cgcacttcgt
     4501 ggttcagcta acctcctcgc cgatctcctt cttccgcctg gcgatgaagt ccatcagcgc
     4561 ctcgtccacg gcggggtcga gcggcggcgc ttggtattcg gccagcatct tcttccaggt
     4621 ggcgttcgcc cgctgggccg tgtcgaggcc gc
//
LOCUS       DASWIY010000355         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_234461, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000355 DASWIY010000000
VERSION     DASWIY010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234461"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..1691)
                     /locus_tag="VGA59_12640"
     CDS             complement(327..1691)
                     /locus_tag="VGA59_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX9720568.1"
                     /translation="MRPLIVKQLNYDLTPVAGLSLVGHHLKRLSPVFEQIDKALPVRT
                     GVSTSDIVRSYVGLLVQGKSDFDAMENFRGDAFFKQSLGIGLLPSSVTLRQRLDAQAA
                     ELFDFVPPLIETVLGRARPDYGVLPCGWLPLDIDTFAMDNGGTVKEGVGRTYAGVDGY
                     CPLAAYLGAHGFCLELALRPGVQHSSLETAFNFERVIPMAQRLSAAGPKAPILLREDS
                     GFDSAAQMQAIESYNRAGMPQVDWLIKWNPRKTDVAEWAAQRDADPGTRWEHPRRGKR
                     VTMWEEAVSLEGVQRPVRRVLRLIERTIDGRGQHLIQPEFQLDGWTTSLSADRFSSPD
                     IIALYADHGTHEQFHSEFKTDLDLERLPSGKFDTNYVVSGLAALTMNILRLMGQAGLH
                     GPDAPVRHPAKRRRLKTVMQELVYRAGRLINHGRRLILGLGANDRAAKAFMRVQGELF
                     AACG"
     gene            1815..1997
                     /locus_tag="VGA59_12645"
     CDS             1815..1997
                     /locus_tag="VGA59_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720569.1"
                     /translation="MITTNPQIDSGSDAQRDTLAAVMEGTVAMVLRQLLEWLRMASSP
                     TLATNDDLPNSKSMAT"
BASE COUNT          371 a          688 c          686 g          366 t
ORIGIN      
        1 ctcatgtctt cacacggcga gagttcttga agtcaaaaaa cacaacagcc gctcttgacg
       61 aaaggggaag tccttgtaga aggcctggat ccgttggctc aatcccggcc tgatcgacat
      121 tcggcatctc aatgcggatc gtcacgtcca atttgatgcc aatgcgccaa ttcaagtgac
      181 ctgatcagcg attcgggttg ccagggcgta tggtgatgcc gccgatacga cagcggcggc
      241 ccgagttgct cacgccgcaa cgcgcgctgc gtgcgcgatt ttcgggatcc gtggggagta
      301 acaggcttgt cgaggtgtgc ggcgcactac ccgcaggcgg cgaacaactc gccttgcact
      361 cgcatgaagg ccttggcggc tcgatcgttg gcgcccaagc ccaggatcag gcgccgcccg
      421 tgattgataa gtcgcccggc gcggtagacg agttcctgca tgacggtctt cagccgccgc
      481 cgcttggccg ggtggcgcac cggcgcatcc ggcccatgca gcccggcctg gcccatcagg
      541 cgcaggatgt tcatcgtcag cgcggccagc ccgctcacga cgtagttggt atcgaacttg
      601 cccgagggca gccgctccag atcgaggtcg gtcttgaatt ccgagtggaa ctgctcgtgc
      661 gtgccgtggt cggcgtacag cgcgatgatg tcggggctgc tgaacctgtc ggcgctcagg
      721 ctggtggtcc agccgtcgag ttggaactcg ggctggatca ggtgctgccc acggccatcg
      781 atggtgcgct cgatcaggcg cagcacccgg cgcaccggcc gctggacgcc ttcgaggctc
      841 accgcctctt cccacatcgt cacgcgcttg ccgcggcgcg ggtgctccca ccgcgtgccc
      901 gggtccgcat cacgctgcgc ggcccactcg gccacgtcgg tcttgcgcgg gttccacttg
      961 atgagccagt ccacctgcgg catgccggca cggttgtacg actcgatggc ctgcatctgg
     1021 gctgcgctgt cgaagcccga atcctcgcgc agcaggatcg gcgccttggg tccagccgcg
     1081 ctcagccgct gcgccatcgg gatcacgcgc tcgaagttga aggccgtctc cagcgacgaa
     1141 tgctgcacgc ccgggcgcag tgccagttcc aggcagaacc cgtgcgcgcc caggtacgcc
     1201 gccagcgggc agtagccgtc cacgccggcg taggtgcggc ctacgccttc cttgacggtg
     1261 ccgccgttgt ccatcgcaaa ggtgtcgatg tccagcggca gccaaccgca cggcagcacg
     1321 ccgtagtcgg ggcgtgcccg gccgagcacg gtctcgatca gcggcgggac gaagtcgaac
     1381 agttcggccg cttgcgcatc caggcgctgg cgcagcgtca cgctcgacgg cagcagcccg
     1441 atgcccagcg attgcttgaa gaacgcgtcg ccgcggaagt tctccatcgc gtcgaagtcg
     1501 gacttgcctt gcacgagcag cccgacgtag ctgcgcacga tgtcgctggt gctcacgccg
     1561 gtcctgaccg gcaaggcctt gtcgatttgc tcgaacaccg gcgacagccg cttcaagtgg
     1621 tggcccacca gcgaaagccc ggccacgggc gtgaggtcgt agttgagttg cttgacgatc
     1681 aatgggcgca tgggccgcga gtagatcacg gccgacgcga tcgctcagca acgcattgca
     1741 accgcttcgc cgtgcacttg cggccgcacc ctcagggtga acgccgctcc tcgtgaggtc
     1801 acggattcag gcacttgatc accacaaatc cacaaattga ctcgggttca gatgctcagc
     1861 gcgacacttt ggcagcagtc atggaaggca cggtggcgat ggtgctcaga caactgctcg
     1921 agtggttgag gatggcaagt tcgcccacgc tcgcgacgaa cgacgacttg ccgaactcga
     1981 agagcatggc gacgtgagaa acgcatgcgg cggcgcaagg aggaactcga aacactcgag
     2041 tgcaccgaac ttgcggccca gcgatggctg cgtgcccaga tgttccgggg ctacgcaggc
     2101 tgccgccaag a
//
LOCUS       DASWIY010000356         8759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_611741, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000356 DASWIY010000000
VERSION     DASWIY010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611741"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..668)
                     /locus_tag="VGA59_12650"
     CDS             complement(201..668)
                     /locus_tag="VGA59_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX9720570.1"
                     /translation="MQDWLAALLAWLALPQYGLSTLFVAAFVSATLLPMGSEPALFGL
                     LRLNPDLFWSAIFVATAGNTLGGVVDWWMGYEAHKVVDKYHHSRSHMKAVEWLEQLGP
                     KACLLAWLPILGDPLCAVAGWLKMSFWPCVGYMAIGKFLRYLTMTVALLWVFP"
     gene            880..1917
                     /gene="dusB"
                     /locus_tag="VGA59_12655"
     CDS             880..1917
                     /gene="dusB"
                     /locus_tag="VGA59_12655"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX9720571.1"
                     /translation="MNIGPYPLANNLFAAPMAGVTDRPFRQLCRKLGAGYAVSEMVTS
                     RRELWASLKTSRRADHEGEPGPIAVQIAGTDAAMMAEAAAYNVERGAQIIDINMGCPA
                     KKVCNKWAGSALMQDEALAIAIARAVVKECEPRGVPVTLKMRTGWSQAHRNAVKLARA
                     FEDAGVRMLIVHGRTREQGYGGQAEYDTIAAVKAAVRVPVVANGDINTPEQAREVLAC
                     TNADAIMIGRAAQGRPWIFREIAHFLATGVHLAPPLVAEVRRLLLEHLRDHYGLYGEF
                     TGVRSARKHIGWYVKALPGGAAFRARMNSIEDSAGQLQAVAAFFDALASIMDRMPATT
                     AVVAGEEEAIA"
     gene            1914..2150
                     /locus_tag="VGA59_12660"
     CDS             1914..2150
                     /locus_tag="VGA59_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEX9720572.1"
                     /translation="MSKKHIEECVRASLEGYFRDLRGTEPDGMYEMLVKVVEKPLLEV
                     VMAKAEQNQSKAAEWLGLNRNTLRKKLLEHRLLK"
     gene            2147..3718
                     /gene="purH"
                     /locus_tag="VGA59_12665"
     CDS             2147..3718
                     /gene="purH"
                     /locus_tag="VGA59_12665"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX9720573.1"
                     /translation="MNALISVSDKTGIVEFARALHALGIRLISTGGTAGLLAGEGLPV
                     TEVAQLTDFPEMLDGRVKTLHPKVHGGLLARRELPEHMAALKAHGIDIIDLLVVNLYP
                     FEATVAKPGCTLEDAIENIDIGGPAMVRSAAKNWKDVGVLTDASQYPLVLEELQSDGK
                     LSDRTRFALSVAAFNRISNYDAAISDYLSSIQEGGGRALFPSQANGRFVKLQDLRYGE
                     NPHQQAAFYRDLYPAPGSLVTARQLQGKELSYNNIGDADAAWECVKGFDEPACVIVKH
                     ANPCGVAVGKDPLEAYSKAFKTDSTSAFGGIIAFNRAVDGAAAQAVAKQFVEVLMAPD
                     FTAQALDMFKGKVNVRILQIAMPPGGPSDWDNGRNAMDIKRVGSGLLMQTADNRELNL
                     AELKVVTKKQPTAQQLQDLLFAWKVAKFVKSNAIVFCKDGMTMGVGAGQMSRLDSARI
                     ASIKAQHAGLSLQDTVVASDAFFPFRDGLDVVVDAGATCVIQPGGSMRDEEVIAAADE
                     RGVAMVFSGVRHFRH"
     gene            complement(3972..5276)
                     /gene="hemL"
                     /locus_tag="VGA59_12670"
     CDS             complement(3972..5276)
                     /gene="hemL"
                     /locus_tag="VGA59_12670"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX9720574.1"
                     /translation="MDQNESLFERAKALIPGGVNSPVRAFRAVGGTPRFVARAQGAYF
                     WDANGKRYVDYIGSWGPMILGHGHPAVVEAVQKAVLEGFSYGAPTEREVALAEEIVSL
                     VPSIEMVRLVSSGTEAAMSAIRLARGATGRSKLIKFEGCYHGHADALLVKAGSGLATF
                     GNPTSAGVPPEVVQHTLVLEYNNLAQLEEAFALHGADIACLIIEPIAGNMNFVRCGVP
                     FMRRCRELCTQHGALLVFDEVMTGFRVALGGAQSVYAKAIPGFRPDLSVLGKVIGGGM
                     PLAAFGGPRAIMEQLAPLGPVYQAGTLSGNPVATACGLATLKEIRKAGFFDGLAGKTA
                     TLVEGLRSAAAAEGVAFSADCEGGMFGFFLLPVLPQNYSQVMKSDSPRFNLLFHGLLD
                     RGVYIAPALYEAGFVSAAHSEDDIAQTISTAREVFKTLSSPA"
     gene            complement(5417..6355)
                     /locus_tag="VGA59_12675"
     CDS             complement(5417..6355)
                     /locus_tag="VGA59_12675"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX9720575.1"
                     /translation="MTNPKFPTAPSEPPEGEEDDTNPACVMVFNASDPSGAGGLSADV
                     TAVASVGAHALPVVTGAYARDTAEVFDHFAFDEEVVSEQARTILEDAPVQVIKVGFVG
                     SPENISTIAEIAADYSEVPLVAYMPDLSWWDEGQIDLYLDAFRELMLPQTTVLVGNHS
                     TLWRWLLPDWSSDRSPTARDIAKAASEMGVPYTLVTGIPLPEQFIDNVLASPQAVLCS
                     EKFERFEAMFSGAGDTLSASLAALVASGTDLAAAATEALGYLDRCLDAGFRPGMGNVV
                     PDRLFWAQPEGETETLTEEEAQALQVFDMPAHDTKH"
     gene            6471..6623
                     /locus_tag="VGA59_12680"
     CDS             6471..6623
                     /locus_tag="VGA59_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX9720576.1"
                     /translation="MCLICGWIYDEAAGAPDHGIAPGTAWSEVPMNWTCPECGARKED
                     FEMVQI"
     gene            6693..7073
                     /locus_tag="VGA59_12685"
     CDS             6693..7073
                     /locus_tag="VGA59_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9720577.1"
                     /translation="MSTTGFKVLVIDDSNTIRRSAEIFLKQGGHDVLLAEDGFDALAK
                     VNDYQPNLIFCDILMPRLDGYQTCAIIKRNAKFASTPVVMLSSKDGVFDKARGRMVGS
                     QDYLTKPFTKDQLLQTVRQFGAAL"
     gene            7085..7450
                     /locus_tag="VGA59_12690"
     CDS             7085..7450
                     /locus_tag="VGA59_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9720578.1"
                     /translation="MAIQKILVVDDSKTELMFMTDMLQKHGFSVKTAENAEDAFRRLA
                     EEKPDLILMDVVMPGQNGFQLTRAITRDPLYADVPIIMCTSKNQETDRVWGMRQGARD
                     YITKPVDADELVTKIKALG"
     gene            7469..7999
                     /locus_tag="VGA59_12695"
     CDS             7469..7999
                     /locus_tag="VGA59_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX9720579.1"
                     /translation="MANREALRELQSRLASRLQAARTEGVQASWLAVESAGARYLFPL
                     AQSGEIFPFVNAQPVPYTQAWFLGVANLRGGLYGVVDLAGFVAGRAPAMRSDATRAES
                     RLVALNSMLEVNCALLIERLAGLRNLDAFSSSAQPPEGSPGYFGSGYTDANGTYWQEI
                     NLQALSQQPQFLSISA"
     gene            8014..>8759
                     /locus_tag="VGA59_12700"
     CDS             8014..>8759
                     /locus_tag="VGA59_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HEX9720580.1"
                     /translation="MSVVVLLKNLFAKKPVEAPVETSGELSLGMPDASLDPMMVTGGM
                     EATGTKKVAVTPIDPDSVAQDDSVAPPEEAAAAELISVPLLGRRTIVTHQRLLFTFLA
                     LALVVLGSVAGFAVRQADTVAQQVAGTGQSLMQSQRLAKSVSQALVGSAQAFPDVKES
                     ADVLAKTVRGLQAGDAALRLEPVQSDMQEDVTKIVPLMERAEKNAGVVMGQQKILTQV
                     GAALRTINRQSSDLLEIAETVSSLKLQQNAP"
BASE COUNT         1604 a         2802 c         2904 g         1449 t
ORIGIN      
        1 gcgctgattt gttccctatg gcgggccagc accggagccg ccccgcgttt ttcgcaggat
       61 acgcgattca tgcattgcca tccactgcca gcgcatctgc cggaaagatc cacagtagca
      121 ccgccgtcat cgtgacatgg cgcaagaact tggcaattat ccacggctca ggcctgcacg
      181 accgtggcgc agccgccgca tcacggaaat acccagagca gcgcgaccgt catcgtcagg
      241 tagcgcagga atttcccgat cgccatgtag cccacgcagg gccagaacga catcttgagc
      301 cagccggcca cggcgcacag cgggtcgccg aggatcggca gccaggccaa caggcaggct
      361 ttgggtccga gctgctccag ccattcgacg gctttcatat gcgagcgcga atggtggtac
      421 ttgtccacca ccttgtgcgc ctcgtagccc atccaccagt cgacgacccc gcccagggta
      481 ttgccggcgg tggccacgaa gatggcgctc cagaacaggt cggggttcag ccgcagcaag
      541 ccgaacagcg ccggctccga acccatgggc aacagcgtcg ccgacacgaa tgccgcgacg
      601 aacaatgtgc tcagcccgta ctgcggcagg gcgagccagg ccaggagcgc agcgagccag
      661 tcctgcatct cagggttcgt ccggtcgccc gcagcgcggc ggcagcctgg tcggagggca
      721 acggacgccg cgtgcatggg ggtgcttcat tcgggaccag tataggcagg cgccgcgcgt
      781 gaacttgtag gcagccacgc agttatcatt tcaaccgctg aaatgcgccg ggcatacaat
      841 cgtgcctcat ttttcagcag tccggctttt ccccttcgca tgaacatcgg cccctaccca
      901 ctggcgaaca acctgttcgc ggcgccgatg gcaggcgtca ccgaccggcc gttccgccag
      961 ttgtgccgca agctcggcgc cggctacgcg gtcagcgaga tggtgacgtc gcgccgcgag
     1021 ctgtgggcga gcctcaagac gtcgcggcgc gccgaccacg agggcgagcc cgggcccatc
     1081 gcggtgcaga tcgcgggcac cgacgcggcg atgatggccg aggctgccgc ctacaacgtg
     1141 gagcgcggcg cgcagatcat cgacatcaac atgggttgcc cggccaagaa ggtgtgcaac
     1201 aaatgggcag gctcggcgct gatgcaggac gaggcgctgg cgatagcgat cgcgcgggct
     1261 gtggtcaagg aatgtgagcc gcgcggcgtg ccggtgacgc tcaagatgcg caccggctgg
     1321 agccaggccc acagaaacgc ggtgaagctc gcccgtgcct tcgaagacgc cggcgtgcgc
     1381 atgctcatcg tgcacgggcg cacccgcgag cagggctatg gagggcaggc ggagtacgac
     1441 accatcgccg ccgtcaaggc cgcggtgcgc gtgccggtcg tggccaacgg cgacatcaac
     1501 acgcccgagc aggcacgcga agtcctggct tgcacgaatg ccgacgccat catgatcggc
     1561 cgggcggcgc aagggcgccc ctggatcttt cgcgagatcg cccatttcct ggcgacgggt
     1621 gtccatctcg cgccgccgct ggtggccgag gtacggcgct tgctgctcga gcatttgcgg
     1681 gatcactacg gcctgtacgg cgaattcacc ggggtgcgca gcgctcgcaa gcacatcggc
     1741 tggtacgtga aggcgctgcc cggcggcgct gccttccgcg cgcgcatgaa ttcgatcgaa
     1801 gacagcgccg ggcagttgca agcggttgcc gcgttcttcg acgcactcgc tagcatcatg
     1861 gaccggatgc ccgcgaccac ggcggtcgtg gctggagaag aagaagcgat cgcatgagca
     1921 agaagcacat agaagaatgc gtgcgcgcga gcctggaggg gtatttccgg gacctgcgtg
     1981 gaaccgaacc cgacggcatg tacgagatgc tggtcaaggt ggtggaaaag ccgctgctcg
     2041 aagtcgtcat ggcgaaggcc gagcagaacc agtcgaaggc ggccgaatgg ctggggctga
     2101 accgcaacac gctgcgcaag aaactgctgg aacacagatt actaaaatga acgcactcat
     2161 ctccgtttcc gacaagaccg gcatcgtcga attcgcccgc gcgctccatg cgctgggtat
     2221 ccgtctgatc tccaccggcg gcaccgccgg gctgctggcc ggcgagggct tgccggtgac
     2281 cgaagtcgcg caactcaccg actttccgga gatgctggac gggcgggtca agacgctgca
     2341 tcccaaggtg catggcggcc tgctggcgcg gcgcgagctg cccgagcaca tggccgcgct
     2401 caaggcgcac ggcatcgaca tcatcgacct gctggtggtc aacctgtatc cgttcgaagc
     2461 cacggtggcc aagcccggat gcacgctgga ggacgcgatc gagaacatcg acatcggcgg
     2521 ccccgcgatg gtgcgctcgg cggcgaagaa ctggaaagac gtgggcgtgt tgaccgacgc
     2581 gtcgcaatat ccgctggtgc tggaagaact ccagtccgac ggcaagcttt ccgatcggac
     2641 gcgattcgcg ctttcggtgg cggcgttcaa ccgcatcagc aattacgacg ccgcgatcag
     2701 cgactatctg tccagcatcc aggaaggcgg cggccgcgcc ctgttcccgt cgcaggccaa
     2761 cggccgcttc gtcaagctgc aggacctgcg ctatggcgag aatccgcacc agcaggcggc
     2821 gttctatcga gatctgtatc cggcacccgg ttcgctcgtc acggcccggc agctgcaggg
     2881 caaggagctg tcgtacaaca acatcggcga cgcggacgcc gcatgggagt gcgtcaaggg
     2941 cttcgacgag cccgcctgcg tgatcgtgaa gcacgccaat ccttgcggcg tcgcggtggg
     3001 caaggatccg cttgaggcgt acagcaaggc gttcaagacc gattccacgt ccgccttcgg
     3061 cggcatcatc gctttcaatc gcgcggttga cggggcggcg gcgcaggccg tggccaaaca
     3121 gttcgtcgaa gtgctgatgg cgcccgattt cacggcgcag gcgctggaca tgttcaaggg
     3181 caaggtcaac gtgcgcatcc tgcagatcgc gatgccgccg ggcggcccca gcgactggga
     3241 caacggccgc aacgcgatgg acatcaagcg cgtcggttcg ggcctgctga tgcagaccgc
     3301 cgacaaccgc gaactgaacc tcgccgagct gaaggtcgtc acgaaaaagc agccgaccgc
     3361 ccagcagctg caggacctgc ttttcgcctg gaaggtcgcg aagttcgtca agtccaatgc
     3421 catcgtgttc tgcaaggacg gcatgacgat gggtgtggga gccgggcaga tgagccgcct
     3481 cgactcggcc cgcatcgcga gcatcaaggc ccagcatgcc ggcctgtcgc tgcaggacac
     3541 ggtggtggcc agcgacgcgt tctttccgtt ccgcgatggg ctggacgtgg tcgtcgacgc
     3601 gggcgcgacc tgcgtcatcc agcccggggg cagcatgcgc gacgaggagg tgatcgccgc
     3661 cgccgacgag cgcggcgtgg cgatggtgtt ttcaggcgtg cggcacttca ggcactgatc
     3721 tggatgtcat cccgggctcg catgactttg aagccggaag tcatggattg cgggtcaagc
     3781 ccgcaatgac aaaggctgtc atcccggact tgatccggga tccatgaatt cagaagtcat
     3841 ggattgcggg gccggagcgt gatgcctcgg cacgtgcata gcgtaggccc ggcattgggc
     3901 tcgctgcact ggacagctgc gcgccccggg cgttcctcga gcccagcccg caatgacatt
     3961 cccagatgcg gtcaagccgg cgacgacagc gtcttgaaca cctcgcgcgc cgtcgaaatc
     4021 gtctgcgcga tgtcgtcctc gctgtgcgcg gcacttacaa acccggcttc gtacagcgcc
     4081 ggcgcgatgt agacgccgcg atccagcagc ccgtggaata gcaagttgaa ccggggcgag
     4141 tcgctcttca tgacctgcga atagttctgc gggaggacgg gcagcaggaa gaatccgaac
     4201 atgccgccct cgcagtcggc gctgaacgct acgccttcgg cggcggcggc ggacctcagg
     4261 ccctcgacca gtgttgcggt cttgcccgcc aaaccatcaa aaaatcccgc tttccggatt
     4321 tctttcaaag tcgccaaacc gcaggccgtg gccaccggat tgcccgatag cgtgcccgcc
     4381 tgatacaccg gcccgagcgg ggccagctgc tccatgatgg cccgcggtcc gccaaaggcg
     4441 gccagcggca tgccgccgcc gatcaccttg cccagcaccg agaggtccgg ccggaatccc
     4501 ggaatggcct tggcgtagac gctctgggcg cctcccaggg ccactcgaaa gccggtcatc
     4561 acctcgtcga acaccagcag cgcgccgtgc tgcgtgcaca actcgcggca gcggcgcatg
     4621 aacgggacgc cgcagcgaac gaaattcatg ttgccggcga tcggctcgat gatcaggcag
     4681 gcgatgtccg caccgtgcag cgcgaacgct tcctcgagct gcgccaggtt gttgtattcg
     4741 agcaccagcg tgtgctgcac cacttcgggc ggcacaccgg cactggtggg attgccgaac
     4801 gtggccaggc ccgagccggc cttgaccagc agcgcgtcgg catgaccgtg gtaacagccc
     4861 tcgaacttga tcagcttgct gcggccggtg gcgccgcggg ccaggcggat cgcgctcatc
     4921 gcggcctcgg tgcccgagct caccaggcgc accatctcga tcgagggcac gaggctcacg
     4981 atctcctcgg ccagcgccac ctcgcgctcg gtcggcgctc catacgagaa gccttccagc
     5041 acggctttct gcaccgcctc caccacggcg ggatggccgt ggccgaggat catcgggccc
     5101 caggagccga tgtagtcgac gtagcgcttg ccgttcgcgt cccagaagta cgcgccttgc
     5161 gcgcgcgcga cgaatcgcgg cgtgccgccg acggcgcgga acgcgcgcac cggcgagttc
     5221 acgccaccgg ggatcagcgc tttggcgcgc tcgaataaag actcgttttg atccatggtt
     5281 tcaacgcttg cgcggtacgt gcggggccgc cgcggatccg gctttgccgg gccgctggag
     5341 gcgccccctt gagggggaga gggcgaagcc cgagggggca tcggccaaag gccgcttcgg
     5401 gggtgggcta agtctttcaa tgcttggtgt catgggcggg catgtcgaat acttgcaggg
     5461 cctgtgcctc ttcctcggtc agggtctcgg tctcgccttc gggctgggcc cagaacaggc
     5521 gatcgggcac gacgttgccc atccccggcc ggaagccggc gtccaggcat cggtcgaggt
     5581 agcccagggc ttcggtggcg gccgcggcca ggtccgtgcc gctggccacc agggccgcga
     5641 gcgacgccga cagggtatcg ccggcgccgg aaaacatcgc ttcgaaacgt tcgaacttct
     5701 cgctgcacag cacggcttgc ggcgacgcca gcacgttgtc gatgaactgc tcgggcaagg
     5761 ggatgcccgt gaccagcgtg tacggcacgc ccatctccga ggcggccttg gcaatgtcgc
     5821 gcgccgtggg gctgcggtcg ctgctccagt caggcaacag ccagcgccag agcgtgctgt
     5881 ggtttcctac caacacggtt gtctgtggca gcatgagctc gcggaaagcg tccaggtaca
     5941 ggtcgatctg gccttcgtcc caccatgaca ggtcgggcat gtaggcgacc agcgggactt
     6001 ccgagtagtc ggcggcgatc tccgcgatcg tgctgatgtt ctcggggctt ccgacgaagc
     6061 ccaccttgat cacttgcacc ggcgcgtcct cgaggatggt gcgggcctgc tcggacacga
     6121 cttcttcgtc gaaggcgaaa tggtcgaata cctcggcggt gtcgcgcgca tacgcgccgg
     6181 ttacaaccgg cagcgcgtgc gctcccaccg acgcgaccgc cgttacatct gccgaaaggc
     6241 cgccggcgcc actgggatcg ctcgcgttga acaccatcac gcatgccgga ttggtgtcgt
     6301 cctcttcgcc ttcggggggc tcggatggtg cggtcgggaa tttggggttt gtcataggcc
     6361 tggatcatcc agccattggc gggctgtgcg ggatagcggc gtaggccctc gatacaatcg
     6421 ttgcattcta ttcgaaggcc ctttaagctg tgaccgaaac gaaaacgtgg atgtgtctga
     6481 tttgcggctg gatatacgac gaagcagccg gagcccccga tcatggtatt gcccccggga
     6541 ccgcctggtc cgaagttccc atgaactgga cttgccccga atgcggtgcc cgcaaggagg
     6601 attttgaaat ggttcagatt taagaggtgg cgcctgttac atcttcggat gatgcagccc
     6661 ccagagaagt tacaaatgag gagcagcgtc aagtgagcac gacgggattc aaggtgctgg
     6721 tgatcgatga cagcaatacc atccggcgca gcgctgagat tttcctgaag cagggcggcc
     6781 acgacgtatt gctggccgag gacggtttcg acgcgttggc gaaagtcaac gactaccagc
     6841 ctaacctgat cttctgcgac atcctgatgc cgcgcctgga cggctaccag acctgcgcga
     6901 tcatcaagcg caatgccaag ttcgcgtcga ccccggtggt gatgctttca tccaaggatg
     6961 gggtgttcga caaggcgcgc gggcgcatgg tcggctcgca ggactacttg accaaaccgt
     7021 ttaccaagga ccagctgctg cagacggtgc ggcagttcgg cgccgcccta taaggagtga
     7081 tgtgatggct atacaaaaaa tcctcgtcgt ggatgattcc aagaccgagt tgatgttcat
     7141 gacggacatg ttgcagaagc acggcttctc ggtcaagacg gcggagaacg ccgaggacgc
     7201 cttccggcgc ctcgcggagg aaaagcccga cctgatcctg atggatgtgg tcatgccggg
     7261 gcagaacggc ttccagctca cgcgggcgat cacgcgcgat ccgctgtatg ccgacgtgcc
     7321 gatcatcatg tgcaccagca agaaccagga aaccgaccgg gtgtggggca tgcgccaggg
     7381 cgcgcgcgac tacatcacca agccggtgga tgccgacgaa ctggtgacca agatcaaggc
     7441 cctgggctga ggcgggtagt cccagacaat ggccaatcgc gaagctctca gagaactcca
     7501 gagccggctg gccagccggc tgcaggcggc ccgtaccgag ggtgtgcagg cttcgtggct
     7561 ggccgtcgag tccgcgggtg cccggtacct gttcccgctg gcccagtccg gggagatctt
     7621 ccccttcgtc aacgcccagc cggtccccta tacgcaggcc tggttcctgg gcgtcgccaa
     7681 cctgcgcggc ggcctctatg gcgtggtcga cctcgcgggc ttcgtcgcgg gccgcgcgcc
     7741 cgccatgcgg tccgatgcga cccgcgctga atcgcgcctg gtcgcgctca actcgatgct
     7801 cgaagtcaat tgcgccctgc tgatcgaacg cctggcaggc ttgcgcaacc tggacgcgtt
     7861 ttcgtcgtcc gcgcaacccc ctgaaggttc gcccggctat tttggcagcg gctacaccga
     7921 tgccaacggt acgtactggc aggaaatcaa cctgcaggcg ttgtcgcaac aaccccagtt
     7981 cctgagcatc agcgcttagt ggaaggctgc accatgtccg tcgtcgtcct actcaaaaac
     8041 ctgttcgcga aaaaacccgt cgaggcgccg gtggaaacga gcggcgagct cagcctgggc
     8101 atgccggatg cgtccctcga cccgatgatg gtcacgggcg ggatggaggc gaccggaacc
     8161 aaaaaggtcg cagtgacgcc gatcgatccc gacagcgtgg cgcaggacga ttcggtcgca
     8221 cctccggaag aagccgccgc ggcggaactg atctcggtac ccctgctggg acgccgcacc
     8281 atcgtcacgc accagcggtt gctgttcacg tttctggctc tcgcgctggt ggtgctgggt
     8341 tcggtagccg gcttcgcggt gcggcaggcc gacacggtcg cccagcaggt ggccggtacc
     8401 ggccagtcgc tgatgcagtc gcaacgcctt gccaagtcgg tgtcgcaggc cctggtgggc
     8461 agcgcgcagg ccttccccga cgtgaaggaa agcgccgacg tgctggccaa gacggtgcgc
     8521 ggcctgcagg ccggcgacgc ggccctgcgg ctggagccgg tgcagtcgga catgcaggag
     8581 gacgtgacca agatcgtgcc gctgatggag cgcgccgaaa agaacgccgg cgtcgtgatg
     8641 ggccaacaga agatcctgac gcaggtgggc gccgccctgc gcaccatcaa ccgccagtcg
     8701 tccgacctgc tggaaatcgc cgagacggtg tcgtcgctga agctccagca gaacgcgcc
//
LOCUS       DASWIY010000357         6267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3550250, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000357 DASWIY010000000
VERSION     DASWIY010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3550250"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1707)
                     /locus_tag="VGA59_12705"
     CDS             complement(<1..1707)
                     /locus_tag="VGA59_12705"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX9720581.1"
                     /translation="MRFRFGIGVTSVLATAILALAPLGAWAQSVKGTVTNGSRAVASA
                     TVYLVPAADVAKLGKAPSNDIRRNVNDDEPMEDNLATNRDKYPNGVTNSKGQFTIAKA
                     AAGKYFVYVEPADKTYLPGGTLANKSQSLADLSAKPLEIQVSGNVPANATFVGSSKCL
                     GCHEDNENIKKTAHKLGISVVGKPSKLQDTSRFPGMNDGLKKLQAGLKLYLYGFDKTR
                     SFDKYLIATAAPADPASVSFTATFFTDKDGSLKLRTENARDPSDPPRVYPVERTYGGP
                     VYKQRYLYRVGDSTYPFLQYNSEGKDAYADRTRKVWRDYHGDWLFSEASNKLIDPPKS
                     KSFEIQCASCHYNGYTLTPTVEGGFVAGAVNDPNGEADIDGDGVANELNIGCETCHGA
                     GSEHAKAKSSLKPSLIVNPAKLAAERATVICTQCHSRPQGTMKNDQPISKENKMLTPG
                     ISRNEYLVNHTTREDAAQKDLWGDGVHSKAHHQQGTDFVRSKHYTNGTQTLSCANCHD
                     PHGNSGLKHQTVKAVRDGQNSLCASCHKVSLKEHTTKSVGTAHTDKIACVDCHMTKTM
                     QTG"
     gene            complement(1905..3269)
                     /locus_tag="VGA59_12710"
     CDS             complement(1905..3269)
                     /locus_tag="VGA59_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9720582.1"
                     /translation="MKSKILLVDDDPFTRRLFQNLFRDGAIDLVTATSVAEARSAYDL
                     GDFNLVILDQRLPDGNGLDLFAEMRKLRPRQTALMITGYAEVRDAMRAVREGLFDYLS
                     KPFENLEELEAVISKALEMDRAYREIADLREIFTSGSGDPIIISHSVAMEKLLAQLRQ
                     AAPLDTTVLVEGESGTGKEVIAKLLHSLSARARGPLKALNCGALSEPLIEAALFGYEK
                     GAFTGAVKTTAGYFEEADNGTILLDEIADMSPKLQVSLLRVLQEGTFVRLGSTVQRPS
                     NFRLICATNKPLDAEVAAGRFRSDLYYRINVVPLRVPPLRERRDDILPLALFFLDHFN
                     RKFNKTAGPLQPDAVASLEGAPWAGNVRELKHTIERAVVINAGGPISSGDLGIDTAAS
                     DAAVLPTQALPYREAHLRFERTYFANLLRLADGNVSEASRLSGIARQNIYGHLERAGV
                     VTKK"
     gene            complement(3266..4663)
                     /locus_tag="VGA59_12715"
     CDS             complement(3266..4663)
                     /locus_tag="VGA59_12715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9720583.1"
                     /translation="MFKLTTPPKMFAAMSLLMIVATVVGTGLVQAPFFRQAIIDREAA
                     MVRDMVNALASEQGIAAQDLTNYTDPEAMKRLDGSFNTLSKLPGTERIRVFNRDNRIA
                     WSDGDPRRLGMKRTSNMERLSRAMDGQVQAVFNTIRSDINGAQPVAEISESIESYVPI
                     FSQEADASAPPVIGVLALYRDPKDLNRTIRQGLYLLWAVTGVAGLVLFFALHKVFNLV
                     YSRQQSAESKFSKLTLEHERIVQIEKMSAMGELVGEIAHQLNNPLVGVINLTQLAQRQ
                     VRDPQRVSELLAEVRKAGTECRDIVQRILRINQPSRSTLQPTDLSELVQDTIAFCHQS
                     IDLKHSVAYPPPAQKYMLDVDSVLIRQALFNLIHNAILAAPDKQVHVGLEVVQRDAAS
                     GILLSVTDSGPGFSAEEAAKLFKPFFTTRASGTGLGLPVAQHIAMKHGGVVLAENLAT
                     GGARFGIWLPMKTTQ"
     gene            complement(4773..5708)
                     /locus_tag="VGA59_12720"
     CDS             complement(4773..5708)
                     /locus_tag="VGA59_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9720584.1"
                     /translation="MSAAPAASSEMAFFSVLARSGSFSAAARELGVTTPAVSKRLAQL
                     EARLRVALMTRTTRRVGLTPEGEIYLAEARRILAEIDDMERLVTSAVAAPKGLLRVNA
                     TLGFGRSHVAPLIAQFSRAHPEVQVQLQLSVSPPPLGEDAFDVCVSFGAPPDARVIAR
                     LLAPNRRLLCASPGYLDRHGVPRVPHDLAQHSCITIRQGDESYGLWRLSLGKRTEAVK
                     VRGNLSTNDGEIAVNWALTGLGIVMRAEWDIARYLRSGRLRQVLENWQTPPADIHAFY
                     PQRHQTSARVRAFVDFVAGHFAKSSTRAAGLVRQW"
     gene            5816..>6267
                     /locus_tag="VGA59_12725"
     CDS             5816..>6267
                     /locus_tag="VGA59_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720585.1"
                     /translation="MSTGKSVSQSVTAHDKAALEKAINNVNWRVKDLRSFNASGIKER
                     WDPRLEALQKKVNTTLADILGARTPEYKQYAIGALDAALDSMFGDRYSPGELQQAIKD
                     SINQAIGKLNAVKKLLTERLEGRAAATPEPTPEPTPEPTPEPTPEPTP"
BASE COUNT         1060 a         1955 c         2051 g         1201 t
ORIGIN      
        1 gccggtctgc atcgtcttgg tcatgtggca gtcgacgcag gcgatcttgt cggtgtgggc
       61 cgtaccgacc gacttcgtcg tgtgctcctt caggctgacc ttgtggcagc tcgcgcacag
      121 cgaattctgg ccgtcgcgca ctgccttgac ggtctggtgt ttcaggccgc tgttgccatg
      181 cgggtcgtgg cagttcgcgc acgagagggt ctgcgtgccg ttcgtgtagt gcttggagcg
      241 aacgaagtcc gtgccttgct ggtggtgggc cttggaatgc acgccgtcgc cccacaagtc
      301 cttttgggct gcatcctcgc gcgtggtgtg gttcacgagg tactcgttgc ggctgatgcc
      361 gggggtcagc atcttgtttt ccttgctgat cggctggtcg ttcttcatcg tcccctgtgg
      421 ccggctgtgg cactgggtgc agatcacggt ggcgcgctcg gcggcgagct tggccggatt
      481 gacgatcagc gaaggcttca gtgacgactt ggccttggca tgctcggagc cggcgccgtg
      541 gcaggtctcg cagccgatgt tcagctcgtt ggccacgccg tcaccgtcga tgtcggcttc
      601 accgttcgga tcgttcacgg cgccggcgac gaacccgcct tccactgtcg gggtgagcgt
      661 gtagccgttg tagtggcagg aggcacactg gatctcgaag gacttgctct tcggcggatc
      721 aatgagcttg ttgctggcct cgctgaagag ccagtcgccg tgatagtcgc gccacacctt
      781 gcgcgtgcgg tcggcgtagg cgtccttgcc ttcgctgttg tattgcagga acgggtaggt
      841 cgaatctccc acgcggtaca ggtagcgctg cttgtacacg gggccgccat aggttctttc
      901 gaccgggtag acccgtggcg ggtcgctcgg gtcacgcgcg ttctccgtgc gcagcttcag
      961 cgatccgtcc ttgtcggtga agaaggtcgc ggtgaagctg accgaggccg gatcggccgg
     1021 cgcagcggtc gctatcaggt acttgtcgaa gctgcgggtc ttgtcgaagc cgtagaggta
     1081 gagcttcagg ccggcctgca gcttcttcaa cccgtcgttc atgccgggga agcgcgaggt
     1141 gtcctgcagc ttgcttggct tgccgaccac ggagatgccg agcttgtgcg cggttttctt
     1201 gatgttttcg ttgtcctcgt ggcaacccag gcacttggag ctgccgacga atgtcgcatt
     1261 ggccgggaca ttgccggaca cctggatctc cagcggcttg gccgagaggt cggccaggct
     1321 ctgggacttg ttggccagcg tgccgcccgg caggtaggtc ttgtcggccg gctccacgta
     1381 cacgaagtac ttgccggccg cggccttggc aatggtgaac tggcccttcg agttggtcac
     1441 cccattcgga tacttgtcgc gattggtggc gaggttgtct tccatcggct cgtcgtcatt
     1501 gacgttgcgc cgaatgtcgt tcgacggagc cttccccagt ttcgcgacgt cggccgcggg
     1561 taccaggtac accgtcgcgc tggcgacagc gcggctgccg ttcgtcactg tccctttgac
     1621 cgactgtgcc caggcgccca aaggcgccag ggccaggatc gcggtggcga gcacgcttgt
     1681 gacgccaatc ccaaatcgaa atcgcatagc tgtttttctc ctctaactag aatccgcgca
     1741 gccgataagg caggatgccc gccgctgaac gcgcaaacca gtgcaagctg cgtgccaacg
     1801 ggccgaggga ccgattgtcg ctgaaacggc gggcgaaaca agggcggcac cgggccgggc
     1861 tggccgggat gggtcaaact gtcatcacaa aatcatgaca gctgtcattt tttcgtgaca
     1921 accccggcgc gctcgagatg gccgtaaatg ttctggcgtg cgatgcccga aaggcgggat
     1981 gcctccgaga cgttgccatc ggccagccgc agcaaattgg cgaaatacgt acgctcgaag
     2041 cgcaggtgcg cctcgcggta aggcagcgcc tgcgtcggca ggacagcggc gtcggatgcg
     2101 gccgtgtcga tgccaagatc gccgctggaa atgggaccgc cggcattgat caccacggcc
     2161 ctctcgatgg tgtgtttgag ctcgcgcacg ttgccggccc agggcgcgcc ctcgaggctg
     2221 gccaccgcgt ccggctgcag gggcccggcg gtcttgttga atttgcgatt gaaatggtcg
     2281 agaaagaata acgccagggg caggatgtca tcgcgccgtt cgcgcagcgg cggcacgcgc
     2341 aatggcacga cgttgatgcg gtagtaaagg tccgatcgga aacgcccggc ggccacctcg
     2401 gcgtccagcg gcttgttggt cgcgcaaatc agccggaaat tggacgggcg ctgcaccgtg
     2461 ctgccgagcc tgacgaacgt gccctcctgc agcacgcgca gcaggctgac ctggagcttg
     2521 gggctcatgt cggcgatttc gtccagcagg atggtgccgt tgtccgcctc ctcgaagtac
     2581 cccgccgtgg tcttcaccgc gccggtgaag gcgccctttt catagccgaa cagagccgcc
     2641 tctatcagcg gctccgagag cgcgccgcag ttcagcgcct tgagcggtcc gcgagcgcgg
     2701 gcactcaacg agtggagcag cttggcgatc acctccttgc cggtgccgct ctcgccttcg
     2761 acgagcaccg tcgtgtccag cggtgcggcc tggcgcaact gggccaggag cttttccatc
     2821 gcaaccgaat ggctgatgat gatcggatcg cccgaacccg aggtgaatat ctcgcgcaga
     2881 tcggcgatct cccggtaggc gcgatccatt tcgagggcct tggaaatgac ggcttccagc
     2941 tcctcgaggt tttcaaaagg tttgctcagg tagtcgaaca gcccttccct cacggcgcgc
     3001 atcgcgtcac gcacttcggc gtagcccgtg atcatcaggg cggtctgccg cggcctgagc
     3061 ttgcgcatct cggcaaagag atccaggcca ttgccgtccg gaaggcgctg gtcgaggatg
     3121 accaggttga agtcgcccag gtcgtatgcg ctgcgcgctt cagcgacgct ggttgccgtc
     3181 accaggtcga tcgcgccatc gcgaaagaga ttctggaaaa gcctgcgcgt gaacggatcg
     3241 tcgtccacca ggagtatttt ggacttcatt gagttgtctt catcggcagc cagataccga
     3301 acctggcgcc gcctgtcgcg agattttcag caagaaccac gccaccatgt ttcatggcga
     3361 tatgctgcgc caccggcaag cccaggcccg tgccgctggc tcgcgtggtg aagaacggct
     3421 tgaacagctt ggccgcctcc tcggcgctga agccggggcc cgagtcggtc acgctgagca
     3481 ggattccgct tgccgcatcg cgctgcacca cctcgagacc gacatgcacc tgcttgtccg
     3541 gcgcggcgag gatcgcgttg tgaatcaggt tgaaaagcgc ctgccgaatg agcacggagt
     3601 ccacatcgag catgtacttt tgcgcggggg gcgggtaggc aacggaatgc ttcaagtcga
     3661 tactctgatg gcaaaaggcg atggtgtcct ggaccagctc gctgaggtcg gtcggctgaa
     3721 gcgtcgaccg cgatggctgg ttgatccgca ggatgcgctg cacgatgtcg cggcattcgg
     3781 tgcccgcctt gcgtacttcg gcgagcagct ccgaaacacg ctgtgggtct cgcacctggc
     3841 gctgggcgag ttgagtcagg ttgatcacac cgaccagcgg gttattgagc tggtgggcga
     3901 tctctccgac cagctcgccc atggccgaca tcttttcgat ctggacgatg cgttcgtgtt
     3961 cgagcgtcag cttgctgaac ttggactcgg cgctttgctg ccggctgtag acgaggttga
     4021 acaccttgtg gagagcgaaa aagaggacca gccccgccac gcccgtcacc gcccacagga
     4081 gatagagacc ttgccggatc gtgcggttca aatccttcgg gtcacgatag agcgcgagca
     4141 caccgatcac ggggggagcg gacgcgtcgg cttcctgcga aaagatcggc acataggact
     4201 cgatcgattc ggaaatttcc gccaccggct gtgcgccatt gatgtcgctc ctgatcgtat
     4261 tgaacacggc ctggacctgg ccgtccatcg cacgtgacag gcgctccatg ttcgaggtgc
     4321 gcttcatgcc cagccgccgg gggtcgccat ccgaccaggc gatcctgttg tcccggttga
     4381 ataccctgat ccgctcggtt ccgggcagtt tgctcaaggt gttgaagctc ccgtcgagcc
     4441 gtttcatcgc ctccgggtcg gtatagttcg tcaggtcctg ggccgcgata ccttgctcag
     4501 aagcgagcgc gttgaccatg tcgcgcacca tcgcggcttc cctgtcgatg atggcctggc
     4561 ggaagaacgg ggcctggacc aggcccgtac cgaccaccgt cgcgacgatc atcagcaggc
     4621 tcatggccgc gaacattttc ggcggggtcg tcaacttgaa catcgcactt cctcatatcc
     4681 ctcggcacgt gccgccaggt gaacgcatga caaccaaagc atcatagggc cggaccggaa
     4741 cccccgactc tcttgccggg gatccgccgc tctcaccatt gacggaccag ccccgccgcg
     4801 cgagtggaag acttggcgaa atggccggcg acgaagtcca cgaaggcgcg cacccgcgcc
     4861 gaggtctggt gccgctgcgg atagaaggcg tggatgtcgg ccggaggcgt ctgccagttt
     4921 tccagcacct ggcgcagccg gccgctgcgc aggtagcgcg cgatgtccca ttcggcgcgc
     4981 atcacgatgc ccagcccggt cagcgcccaa ttcacggcga tctccccgtc gttggtgctc
     5041 aggttgcccc gcaccttgac ggcttcggtt cgtttgccca gcgacagccg ccacagcccg
     5101 tagctttcgt cgccctggcg aatggtgatg caactgtgct gcgccaggtc gtggggcacg
     5161 cgcggcacgc catggcggtc caggtagccg ggcgacgcgc acagcagccg gcggttgggc
     5221 gccagcagcc gggcgatcac gcgcgcgtcg ggcggggcgc cgaagctcac gcacacgtcg
     5281 aacgcatcct cgcccagcgg cggcgggctc acggacagct gcaattgcac ctgcacctcg
     5341 ggatgggccc gggaaaactg ggcgatcaat ggcgcgacgt ggctgcggcc gaatcccagc
     5401 gtcgcattca cgcgcaacaa acccttgggc gcggcgacgg cgctggtcac cagacgttcc
     5461 atgtcgtcga tctcggccag gatgcggcgc gcttcggcca ggtagatctc gccctcgggc
     5521 gtgaggccga cccgccgggt ggtgcgggtc atcagcgcga cgcgcaggcg cgcctccagt
     5581 tgcgccagcc gcttgctcac ggccggcgtc gtcaccccga gctcgcgcgc cgccgctgaa
     5641 aagctgccgc tgcgcgccag cacgctgaaa aaggccattt cggacgatgc ggcgggcgcg
     5701 gcggacattg ttgaactccg ggtcatcaat ggtgtggatt ttcgctcgtc ttttccggcg
     5761 tgccggcccg ccaaggaaga gttaaagtcg gaaaccgtcc acattgggaa gctgcatgtc
     5821 caccggcaaa tcagtgtcgc aatccgttac cgcccacgac aaagctgcgc ttgagaaggc
     5881 gatcaacaac gtcaactggc gtgtcaagga tctgcggtct ttcaatgcct cgggcatcaa
     5941 ggagcgctgg gatccgcgcc tcgaggctct gcaaaagaag gtcaacacga cactggccga
     6001 tatattgggc gcgcgcacgc ccgagtacaa gcagtatgcg ataggcgccc tggacgccgc
     6061 tctcgattcg atgttcggcg accggtactc gccgggcgag ctgcagcaag ccatcaagga
     6121 cagcatcaac caggcgattg gcaagctgaa tgcagtcaag aagctgctga ccgagcgcct
     6181 cgagggcagg gcggctgcga cgcctgagcc tacgcccgag ccgacgcctg aaccgacgcc
     6241 cgagcctacg cctgaaccga cgcctga
//
LOCUS       DASWIY010000358         4508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2106056, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000358 DASWIY010000000
VERSION     DASWIY010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2106056"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(399..2129)
                     /locus_tag="VGA59_12730"
     CDS             complement(399..2129)
                     /locus_tag="VGA59_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9720586.1"
                     /translation="MKMRYTALAIAAACALGSQAWADEAAAKKWVDSEFQPSTMSKEK
                     QMAEMKWFIEAARKLQAKGVKEISVVSETITTHEYESKTLARAFEEITGIKVKHDLIQ
                     EGDVVEKLQTSMQSGRSIYDGWISDSDLIGTHYRYGKVMNLTDYMAGKGKEWTNPGLD
                     LKDFIGTSFTTAPDGKLYQLPDQQFANLYWFRADLFARKDLQDKFKAKYGYDLGVPQN
                     WSAYEDIAEFFTNDVKTIDGKPIYGHMDYGKKDPSLGWRFTDAWLSMAGTADKGIPNG
                     QPVDEWGIRVGTDKCTPVGASVSRGGATNSPAAVYALTKYVDWMKKYAPKDATGMTFG
                     EAGPVPAQGQIAQQIFWYTAFTADMTKKGLPVVNEDGTPKWRMAPGPNGPYWKQGMQN
                     GYQDVGSWTFFAAHDENRTAAAWLYAQFVTAKTTSLKKTLVGLTPIRESDIRSKAMTD
                     MAPKLGGLVEFYRSPARVAWTPTGTNVPDYPKLAQLWWKNVAQAVTGEKTPQGAMDTL
                     AEEMDQVLARLQRAGMAKCPPKLNVKGDPAKFLSDKGAPWAKLANEKPKGETIAYDKL
                     LQAWKDGKVR"
     gene            complement(2227..2535)
                     /locus_tag="VGA59_12735"
     CDS             complement(2227..2535)
                     /locus_tag="VGA59_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HEX9720587.1"
                     /translation="MFDWMVWTTPVAVFFSCIVLMLAGMTAWEIRSPTTLRKGFLPIA
                     TTRGDRLFIGLLTAGYINLAFIGLAGKFTQWFELEQEPSIWISFVVSMGVLALIMRRG
                     "
     gene            complement(2680..3603)
                     /locus_tag="VGA59_12740"
     CDS             complement(2680..3603)
                     /locus_tag="VGA59_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9720588.1"
                     /translation="MSAVVSARASLPLPVGEGRREGTGLPAPSPQPNPLPRGARGKFQ
                     PKTLFLVAYIVFALLPIYWMVNMSFKTNDEILSSFSLLPQHFTWANYRTIFTDVSWYS
                     GYINSLIYVAINTVISVTVALPAAYAFSRYSFLGDKQVFFWLLTNRMTPPAVFLLPFF
                     QLYTTVGLMDTHIAVALAHLLFNVPLAVWILEGFMSGIPREIDETAYIDGYSFPRFFA
                     TIFLPLIKAGVGVAAFFCFMFSWVELLLARTLTSVNAKPIVATMTRTVSASGMDWATL
                     AAAGVLTIVPGAIVIWFVRHYIAKGFAMGRV"
     gene            complement(3600..4490)
                     /locus_tag="VGA59_12745"
     CDS             complement(3600..4490)
                     /locus_tag="VGA59_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9720589.1"
                     /translation="MSTTTKPVNQKAWLLILPVLVCVAFSAILPLMTVVNYSVQDIIS
                     PERRVFVGTEWFAAVMRDEDLHAALLRQLTFSFAVLLVEIPLGIALALSMPAQGWKAS
                     AVLVLIALSLLIPWNVVGTIWQIFGRADIGLLGAALQKIGIDYSYTGNARDAWMTVLA
                     MDVWHWTPLVALLCYAGLRSIPDAYYQAARIDGASKLAVFRYIQLPKMRGVLMIAVLL
                     RFMDSFMIYTEPFVLTGGGPGNATTFLSQYLTQKAVGQFDLGPAAAFSLIYFLIILLL
                     CFILYNWMQRVGTSEKGATQ"
BASE COUNT          791 a         1428 c         1450 g          839 t
ORIGIN      
        1 ttcaagcaga ccttttttaa gcaccctcac cagctttctc tccgccggag acagaatctg
       61 ctggtcgccg atcaaggcga ggcatagggc ggctaagtct tcttccgttt gcatggttat
      121 ttgtacagta tatcgccatg attgcactct gagaacccct gccggaattc tgagatcacc
      181 gacagcgtca ggccaccgct taaacccgcg atacggtgct cgccactcgt tatcgaagcc
      241 acccggcgat tgtcttgaca cgaccaggcc gcgccttttg cgccgacgat gacttgtacg
      301 aattcgacca cggcggtccg cggacagcaa aaaaagccgt gcgaacctgc gcccgcacgg
      361 cttgaaccct caaaggaaac ccgtttcagg gccggcgctt agcgcacctt gccgtccttc
      421 catgcctgca gcagcttgtc gtaggcgatg gtttcgccct tcggcttctc gttggccagc
      481 ttggcccagg gcgcgccctt gtcgctcagg aacttggccg gatcgccctt gacattgagc
      541 ttgggcgggc acttggccat gccggcacgc tgcaggcggg ccagcacctg gtccatttcc
      601 tcagccaggg tgtccatcgc gccttgcggg gtcttctcac ccgtcacggc ctgcgccacg
      661 ttcttccacc acagctgcgc cagcttcggg tagtcgggca cgttggtgcc ggtgggcgtc
      721 cacgccaccc gcgctgggct gcgatagaac tcgaccaggc cgcccagctt gggcgccatg
      781 tcggtcatgg ccttggagcg gatgtcagat tcgcggatcg gggtcaggcc gaccagcgtc
      841 ttcttgagcg acgtggtctt ggccgtgacg aactgcgcgt acagccaggc cgcggcggtg
      901 cggttctcgt catgcgccgc gaagaacgtc cacgagccca cgtcctgata gccgttctgc
      961 atgccctgtt tccagtatgg gccgttcggt cccggcgcca tgcgccactt gggcgtgccg
     1021 tcctcgttca cgaccggcag gcctttcttg gtcatgtcgg cggtgaaggc ggtgtaccag
     1081 aagatctgct gggcgatctg gccttgcgcg ggcaccgggc cggcctcacc gaaggtcatg
     1141 ccggttgcat ccttgggcgc gtacttcttc atccagtcca cgtacttggt cagggcgtag
     1201 acggcggccg gcgagttggt cgcgccgccg cgggagaccg aagcgcctac gggcgtgcac
     1261 ttgtcggtgc ccacgcggat gccccactcg tccacgggct ggccgttcgg gatgcccttg
     1321 tcggcggtgc ccgccatcga cagccacgcg tcggtgaaac gccagcccag cgacgggtct
     1381 ttcttgccgt agtccatgtg gccgtagatc ggcttgccgt cgatggtctt cacgtcgttg
     1441 gtgaagaact cggcgatgtc ctcataggcg ctccagttct gcggcacgcc caggtcatag
     1501 ccgtacttgg ccttgaactt gtcctgcagg tccttgcggg cgaacaggtc ggcgcggaac
     1561 cagtacaggt tggcgaactg ctggtcgggc agctggtaca gcttgccgtc cggcgcggtg
     1621 gtgaagctgg tgccgatgaa atccttcagg tccaggccgg ggttggtcca ctccttgccc
     1681 ttgccggcca tgtagtcggt caggttcatg accttgccgt accggtagtg cgtgccgatc
     1741 aggtccgagt ccgagatcca gccgtcgtag atcgacctgc cggactgcat cgacgtctgc
     1801 agcttctcga ccacgtcccc ttcctggatc aggtcgtgct tgaccttgat gccggtgatt
     1861 tcctcgaagg ccctggccag cgtcttggac tcgtactcgt gcgtcgtgat ggtctcggac
     1921 acgaccgaga tttccttcac gcccttggcc tgcagcttcc tggcggcctc gatgaaccat
     1981 ttcatctcgg ccatttgttt ttctttgctc atggtcgacg gctggaattc gctgtcgacc
     2041 cacttcttcg ccgcggcctc gtcggcccaa gcctggctgc ccagcgcgca cgcggcggcg
     2101 atggccaggg cggtgtatcg catcttcatt ggaatttctc ctcgaatggg tgcttccccg
     2161 tttgcagtga cggtcaccac ggccccgggg aagcatccgg gccggattgc cgtgaacgtt
     2221 gattcgctag ccgcggcgca tgatcagcgc gagcacgccc atggacacca cgaagctgat
     2281 ccagatgctc ggctcctgct ccagctcgaa ccactgggtg aacttgcctg ccagcccgat
     2341 aaaggccagg ttgatgtagc ccgcggtgag caggccgatg aagagccggt cgccgcgggt
     2401 ggtggcgatc ggcaggaagc ccttccgcag cgtcgtgggc gacctgattt cccacgccgt
     2461 catccccgcg agcatcagga cgatgcagct gaagaagacc gccacgggtg tcgtccagac
     2521 catccagtca aacatctcag ctctccataa cgttgcgtgc tgccgcggtc gcatgaacgc
     2581 gaatgcgcca tgccagggcc accgcggaac cggctttgcc gggccgctgg tggcgccccc
     2641 ttgtggggga ggcgccgaag gcgcttcggg ggtgggcgtt catactcttc ccatcgcgaa
     2701 acccttcgcg atgtagtgcc gcacgaacca gatcacgatc gcgccgggca cgatggtcag
     2761 cacgccggcg gccgccagcg tggcccaatc catgcccgat gccgacaccg tgcgtgtcat
     2821 cgttgccacg atcggcttgg cgttgacgct cgtgagcgtg cgcgccagca gcagttccac
     2881 ccagctgaac atgaagcaga agaacgcggc cacgccgaca ccggccttga tcagcggcag
     2941 gaagatcgtc gcgaaaaagc gcgggaacga gtagccgtcg atgtaggcgg tctcgtcgat
     3001 ctcgcgcggg atgccgctca tgaagccctc caggatccac accgccagcg gcacgttgaa
     3061 cagcaggtgc gccagcgcca cggcgatatg cgtgtccatc aggcccacgg tggtgtacag
     3121 ctggaagaac ggcagcagga acacggccgg cggcgtcatg cggttggtga gcagccagaa
     3181 gaacacctgc ttgtcgccca ggaagctgta gcgcgagaac gcataagccg ccggcagggc
     3241 caccgtgacc gagatcacgg tgttgatcgc cacgtagatc agactgttga tatagcccga
     3301 gtaccaggac acgtcggtga agatggtcct gtaattggcc caggtgaagt gctggggcag
     3361 cagcgagaag ctcgagagga tctcgtcgtt cgtcttgaag ctcatgttga ccatccagta
     3421 gatgggcagc aaggcgaaca cgatgtaggc gacgaggaac agcgtcttgg gctggaattt
     3481 ccccctggcc cctctgggga gagggttggg ttgaggggaa ggggcgggca ggcccgtgcc
     3541 ctcacgcctg ccctctccca cagggagagg gagcgaagcc cgtgcggaga cgacagcgct
     3601 cattgcgttg ctcccttttc actggtgccc acccgctgca tccagttgta gaggatgaag
     3661 caaagcagca ggatgatgag gaagtagatc agcgagaacg ccgcggccgg gcccaggtcg
     3721 aactggccga cggctttctg cgtgaggtac tgcgagagga acgtggtggc gttgcccggc
     3781 ccgccgccgg tcaggacgaa gggctcggtg tagatcatga agctgtccat gaagcgcagc
     3841 agcacggcga tcatcagcac gccgcgcatc ttgggcagct ggatatagcg gaacacggcg
     3901 agcttgctgg cgccgtcgat gcgcgcggcc tggtaatagg cgtcgggaat ggaacgcagg
     3961 ccggcatagc acagcagcgc gaccagcggc gtccagtgcc acacgtccat cgccagcacc
     4021 gtcatccagg catcgcgggc gttgccggta tagctgtaat cgatgccgat cttctgcagc
     4081 gcggcgccca gcagcccgat gtcggcgcgc ccgaagatct gccagatggt gccgaccacg
     4141 ttccacggaa tcagcagcga cagcgcgatc agcaccagca cggccgaggc cttccagccc
     4201 tgcgccggca tcgacagcgc caaggcgatg cccagcggga tctcgaccag gagcacggcg
     4261 aacgaaaagg tcagctggcg cagcagcgcg gcgtgcaggt cctcgtcgcg catcacggcg
     4321 gcgaaccatt cggtgccgac gaagacgcgg cgctcgggcg agatgatgtc ctgcaccgag
     4381 tagttcacca cggtcatcaa gggcaggatg gccgagaagg cgacgcagac cagcaccggc
     4441 aggatcagca gccaggcctt ctggttgacg ggtttggtgg tggtgctcat gccaccaact
     4501 cctcatcc
//
LOCUS       DASWIY010000359         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_983073, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000359 DASWIY010000000
VERSION     DASWIY010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983073"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VGA59_12750"
     CDS             complement(<1..179)
                     /locus_tag="VGA59_12750"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9720590.1"
                     /translation="MATTTATTTYHDPSNSSLWDTAALLGRIFMAYLFIPAGWGKIAG
                     FTGVAGYIASKGLPMP"
     gene            complement(204..905)
                     /locus_tag="VGA59_12755"
     CDS             complement(204..905)
                     /locus_tag="VGA59_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9720591.1"
                     /translation="MLTLRKSQERGYADHGWLKSFHSFSFANYFDPQHMGFGNLRVIN
                     EDRIASGTGFGKHGHRDMEIISYVLAGELAHQDTLGNVKGIPPGDVQRMSAGTGVQHS
                     EFNHAKDQTTHFLQIWIQPNVTGIAPSYEQKAFSEGEKRGRLRLVASPDGADGSVLIH
                     ADARMYAGLFDASENFSLVLDPSRKGYVHVARGEVEVNGNRLAAGDAAKLENESQLAL
                     SAGKNAEVLVFDLES"
     gene            1020..1958
                     /locus_tag="VGA59_12760"
     CDS             1020..1958
                     /locus_tag="VGA59_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9720592.1"
                     /translation="MQSPREVLTPDALALLQAVAQAGSFAAAARELGVVPSAVTYRIR
                     QIEDALDVLLFDRSSRQARLTEAGAELLREGGRLLLEIDAVANRVKRVATGWESQLTI
                     AVDTVISCSTVMELAEAFFTLAPNTRLRLREESLSGTLEALTSGQADLALGVVLDTGS
                     SAGIQTSVLGDITFVYVVAPHHPLASVDGPLPDELVQKHRAIAAADSVQRGEALTRGL
                     LAGQDVMTVTTMRAKLDAQLRGLGGGFVPEPMARPYVESGRLVVKEIRRIANRQVRLS
                     YAWRSSPAPGRALQWWLGQLESAATRRALLQQHARA"
     gene            1983..>2812
                     /locus_tag="VGA59_12765"
     CDS             1983..>2812
                     /locus_tag="VGA59_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9720593.1"
                     /translation="MAIQQRKIAVIGAGVAGLSCARTLAQAGHRVTLLEKGSEVGGRM
                     ASCETPFGSFDHGSQYFTVRDERFEKVLSISPGLRKPWSATAVRVLDPHGRVAEAALP
                     AIEPHWVAVPTMDALARHWAQPLMAAGCVEMRTRVTRIERDALDPGRWQLQTSGPGDS
                     VHVFSGFDAVLLAIPSALAAGLLGRSALAQQVRQVEVAPCWTLMLAFPQAVQPTLTHL
                     GPQWNAALSTHHRIAWLARESSKPGRGGVERWTVQASPAWSQEHLEDDGPRVQAKLLK
                     A"
BASE COUNT          451 a          898 c          970 g          493 t
ORIGIN      
        1 ggcatcggca ggcctttgct ggcgatatag ccggcaacac ccgtgaagcc ggcgatcttg
       61 ccccatccgg ccggtatgaa gaggtaagcc atgaagatac ggccgaggag tgccgccgtg
      121 tcccacagcg aactgttgga cgggtcgtgg taggtggtgg tggcggtagt ggtggccata
      181 gcgtctccct ttgaagaaaa aaatcaggat tcgagatcga acaccagcac ttcggcgttc
      241 tttccggcgg acagcgccag ctgggactcg ttctcgagct tggcggcgtc gccggcggca
      301 agccgattgc cgttgacttc gacctcaccg cgcgcaacgt gcacgtagcc ctttcgggaa
      361 gggtccagca ccaggctgaa gttctcgctt gcgtcgaaca gcccggcata catgcgggcg
      421 tcggcatgga tcagtaccga gccgtccgcc ccatcgggcg acgccacaag acgcaggcgg
      481 ccgcgctttt cgccctcgga aaaggccttt tgctcgtagc tcggcgcaat gccggtcacg
      541 ttgggctgga tccagatctg caggaaatgc gtggtctggt ccttggcatg gttgaactcg
      601 ctgtgctgca cgccggtgcc ggcgctcatg cgctgcacat cgcccggcgg gatgcccttg
      661 acgttgccca gcgtgtcctg atgggcgagt tcgcccgcca gcacgtagct gatgatttcc
      721 atgtcgcggt ggccgtgctt gccgaagccg gttcccgatg cgatgcggtc ctcgttgatc
      781 acacgcaaat tgccgaatcc catgtgctgc gggtcgaaat agttcgcgaa cgaaaagctg
      841 tggaacgact tcagccagcc gtggtccgca tagccgcgtt cctgggactt gcgcaaggtc
      901 agcatgaggg tctcttggta atcgcttcca tcttggcaat gtaggagcgc aggggctgtc
      961 agccgacctc tcatttttga tggcatcatt caaaaagttt gaatagagtc gcctcaacca
     1021 tgcaaagccc ccgcgaggtg ctcacgccgg atgccctggc cctgctgcag gcggtggccc
     1081 aggccggcag ttttgccgcg gccgcccgcg aactcggcgt cgtgcccagc gccgtcacct
     1141 atcgcattcg ccagatcgag gatgctctgg acgtgctgct gttcgaccgc agttcgcgcc
     1201 aggcccgcct gaccgaagcc ggcgccgagc tgttgcgcga gggcggccgg ctgctgctgg
     1261 agatcgacgc cgttgccaac cgcgtcaagc gcgtggccac aggctgggag tcccagctga
     1321 ccatcgcggt ggacacggtg atttcctgtt ccacggtgat ggaactggcg gaggcttttt
     1381 tcacgctggc acccaacacc cgcctgcggc tgcgggaaga atcgctgtcc ggcacgctgg
     1441 aggcgctgac ttccggccag gccgacctgg ccctgggggt tgtgctggat accggcagtt
     1501 cggcgggcat ccagaccagc gtgctgggcg acatcacttt cgtctacgtg gtcgccccgc
     1561 atcatccgct ggcgtcggtc gacgggccgc tgccggatga actggtacag aaacaccgcg
     1621 ccatcgcggc ggccgactcg gtgcagcgcg gcgaggcgct cacgcgcggg ctgctggcag
     1681 ggcaggacgt catgacggtg accaccatgc gtgccaagct cgacgcccag ctgcgcgggc
     1741 tgggcggcgg gttcgtgccg gaaccgatgg cgcgtcccta tgtggaaagc ggtcgcctgg
     1801 tggtcaagga gatccggcgc atcgccaatc gccaggtgcg cctgagctat gcatggcgca
     1861 gcagtcccgc ccccggccgg gcgttgcagt ggtggctggg tcagctggag agcgcggcga
     1921 ctcgccgcgc tctgcttcag cagcacgcaa gggcctgatc cgcgctgatg cacaatgaac
     1981 cgatggctat acaacaaaga aagatcgccg tgatcggcgc gggggtcgcg ggcctgtcgt
     2041 gcgcccggac cctggctcag gcggggcacc gggtgacgct gctggaaaag ggcagcgagg
     2101 tgggcgggcg catggccagc tgcgaaaccc cgttcggcag tttcgaccat ggctcccaat
     2161 acttcacggt gcgcgatgag cgcttcgaaa aggtgctgtc gatttcaccg ggcttgcgca
     2221 agccctggag cgcgaccgcg gtgcgggtgc tcgatcccca tggccgggtc gcggaggccg
     2281 ccttgcccgc catcgagccg cactgggtgg cggtgcccac gatggatgcg ctggcgcggc
     2341 actgggctca gcccctcatg gcagccggct gcgtggagat gcgcacacgc gtgacgcgca
     2401 tcgaacgcga tgccctcgac cccggccgct ggcagttgca gaccagcggt cccggggact
     2461 cggtccacgt gttctccggc ttcgacgcgg tactgctcgc cataccgagc gcgctggcgg
     2521 ccggtttgct gggccgatcg gcgctggcgc agcaggtgcg gcaggtggag gtcgctccct
     2581 gctggacgct gatgctggct tttccgcagg cggtccagcc tacgctcacg cacctggggc
     2641 cgcagtggaa cgccgcgctc agcacccatc accgcatcgc ctggctggcg cgggaatcgt
     2701 cgaaaccggg ccgcggcggc gtggagcgct ggacggtgca agccagcccc gcctggtcgc
     2761 aggaacatct ggaagacgat gggccgcgcg tgcaggccaa gctgctcaag gc
//
LOCUS       DASWIY010000360        10548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_984473, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000360 DASWIY010000000
VERSION     DASWIY010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10548
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984473"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VGA59_12770"
     CDS             complement(<1..705)
                     /locus_tag="VGA59_12770"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX9720594.1"
                     /translation="MYHRNILVEQTDPELWSAIQAENRRQEEHIELIASENYASPAVM
                     AAQGTQLTNKYAEGYPGKRYYGGCENVDVAEQLALARVKQLFGADAANVQAHSGAQAN
                     EAVFLAFLKPGDTIMGMSLAEGGHLTHGMALNISGKWFNVVSYGLNDKEEIDYDAMER
                     KAHESKPKLIIAGASAYALRIDFERFAKVAKEIGAIFMVDMAHYAGLIAAGVYPNPVP
                     HADVVTSTTHKTLRGPR"
     regulatory      complement(741..899)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(927..1739)
                     /locus_tag="VGA59_12775"
     CDS             complement(927..1739)
                     /locus_tag="VGA59_12775"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9720595.1"
                     /translation="MTALARTAQGLRTFASDIAQGFFEITHNSFALVGLAVVFSLVGL
                     MARPDLRDAGEIKLRSWLHDRQMAAVGMRIELGAIDRATAVNPQHLPKQQAAVAYWLS
                     RKYRVAPEPLGALVAEAYELGARAKLDPTLILAIMAVESGFNPFAQSSVGAQGLMQVM
                     TGVHSDKYENFGGKLAAFDPVTNLRVGVRVLQESIQRAGSLQAGLKFYVGAANLDSDG
                     GYADKVLAEHARLKHVAAGRGVPLHPPVLRTIAPISPQPQAPEEKVAAVSLS"
     gene            2793..5453
                     /locus_tag="VGA59_12780"
     CDS             2793..5453
                     /locus_tag="VGA59_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEX9720596.1"
                     /translation="MTGGAFSAPTSGERSARVREWLAANPSVEQMQEVFRELSGRDKG
                     AAKLLREKLDEIKRSKGQEAIAAEWAEKARTLLAQDKVNIADAMAWQRDAAKAGAPLS
                     REPLASLKAQLAERIKGIEDLQHRVQVQREAAVLLAQRIEVLSTKSWRDAQAAAEALA
                     GDVPDWQAQAQALLADPNWQSVDNKFPPLLEASRTQLQAVWDAFQSALAQAVAAAEDA
                     SAPLPPVPVWADELRAVRGVAVEATARPAKPKIDPEVRAQATQAVREALAKLEQEIAQ
                     GHGKASAGAAAALRNALKEHGKLIDDKLENQAHAALAAAGELEGWQRWRADQLRQELV
                     AKAEGLFEHSAPVEGQEPQPPKPRYGGRKMQEQLRALREQWKQTDQGGAPNHALWKRF
                     DDACNEAHKVVEAWLDKVKADAAEHRAQRLALIDEVKAWAAENLTARDGDWKGFNRIL
                     HQFEGRWRDAGHLSEKAFAELQPLWKEAIQAAAAPLEAIQKESLERRHAMIDEAKALG
                     AAPQLRVDAVKALQQHWQAEAQAVPLDRKHEQKLWDAFRKPIDDAFNRKGEEREKAAA
                     ALSERDRTVLESSKALEAANATGDVQKIKAAMAALDAALHGQAQAAAAVAAAGPALVA
                     PPAEAPVAEAASADEAAAGPATDEAPAEGEGEAAREPAAPAKPAPKPVIAMRGDDRPG
                     MKKAEPMPAGRGGKFGDRKGGPGGRFGERRDERSGAGGGGRFGDRPAFEDRGPRLGDV
                     AFRAQREALEHAQLTLKKLAAQAHGEALTQLMTAWEQRAADQVPSQQDLGKAVAPAVR
                     SSWVQALGAAPSGDGAEALLRLEIAAEAPTPAEHLSARRALQLQLLTRRNDPSPAQTW
                     GQDAARVLASAYEAGAARRLQGVLKTLLRR"
     gene            complement(5450..6205)
                     /locus_tag="VGA59_12785"
     CDS             complement(5450..6205)
                     /locus_tag="VGA59_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEX9720597.1"
                     /translation="MFSWLRKRRAARDIPDSLWRDLLARYPFLASRPAAELPRLRELA
                     AQFLVTKEFHGANGLVITDAVALAIAAQAVLPVLNLGLAWYDDFVGIVVHPGEVLARR
                     TVTDELGVVHHYNEVLAGEAMEGGPVMLNWSDVDSAGKTAEQGYNVVIHEFIHKIDMR
                     DGAPDGCPPLPTRAARESWLAVMQAEYQAFREKVIIAERFGGEETWLDTYGATGIDEF
                     FAVACEAYFVNRQRFEAEFPALVPLLDSFFRPA"
     gene            complement(6205..6975)
                     /locus_tag="VGA59_12790"
     CDS             complement(6205..6975)
                     /locus_tag="VGA59_12790"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX9720598.1"
                     /translation="MSTVAVPPITELQGPASWHTADVISDMHLHATDPATFEAWRRYM
                     RETPADAVFILGDLFEVWVGDDTAGEPGFAADCASVLQAAAGRMAVFFMHGNRDFLVG
                     EGFLKACRTTLLPDPTVLGFAGRRWLLTHGDALCLADTDYMRFRSQVRAPSWQMEFLS
                     KTLAQRQAIARALREQSEARKRSGVEYPDVDSAAAAAWLDAADAQVLIHGHTHRPADH
                     ALGERRRIVLSDWDAGSKPPRLQALRLTAAGAKRIALS"
     gene            complement(6993..7499)
                     /locus_tag="VGA59_12795"
     CDS             complement(6993..7499)
                     /locus_tag="VGA59_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9720599.1"
                     /translation="MSNPKVELHIAGYGVITLELDQDKAPKSVANFLAYVNKGHYDNT
                     IFHRVIPGFMVQGGGFEPGMKQKPTDAPVENEANNGLKNRNYTVAMARTQDPHSATAQ
                     FFINVADNEFLNHTAPSAQGWGYTVFGKVVGGTDVVDRIKGVKTGRKGFHDDVPNEDV
                     VIEKAVAL"
     gene            complement(7595..8266)
                     /locus_tag="VGA59_12800"
     CDS             complement(7595..8266)
                     /locus_tag="VGA59_12800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9720600.1"
                     /translation="MTPRLAALALSLGLATATGLVAAQEAPKVKFATTAGDIVVEVYP
                     DKAPKTVENFLQYVNDKHYDGTIFHRVIDNFMIQGGGFDSRYIQKPTRPPVAHEGQQA
                     LAKGGPRNVVGTLSMARTSDPNSASSQFFINVKDNASLDPVVIPPGDPVPRFEYKGRV
                     YRNEPRARLAAAQELYGYTVFGKVVSGMDVVNKIKAMPTGAAGPFGSDVPKTPVVITS
                     ATLVK"
     gene            complement(8515..8994)
                     /locus_tag="VGA59_12805"
                     /pseudo
     CDS             complement(8515..8994)
                     /locus_tag="VGA59_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            9210..>10548
                     /gene="cysS"
                     /locus_tag="VGA59_12810"
     CDS             9210..>10548
                     /gene="cysS"
                     /locus_tag="VGA59_12810"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX9720601.1"
                     /translation="MSLRIYNTLSRALEDFSPLEPGHVRMYVCGITVYDLCHVGHARA
                     NVAFDVVQRWLKASGYKLNFVRNITDIEDKIIRRAVENGETVRSLTDRMIAEMYRDFD
                     ALGIERPTHEPRATDYVPQMLDIVRQLEDKDLAYRAEGGDVNYAVRRFPGYGKLSGKT
                     LDELHAGERVAVLEGNKEDPLDFVLWKAAKPTEPDDAQWDSPYGKGRPGWHIECSAMA
                     CALLGKTFDIHGGGADLQFPHHENEIAQSEGANGQPLARYWMHNGFVNIDNEKMSKSL
                     GNFFTIREVLERFDAETLRFFIVRSHYRSPLNYSDVHLDDARASLRRLYNVLADGDAL
                     LHSRGLKASVVPFADLDKSDGMVSAFVRAMDDDFGTPQAISVLFALAGEIGTARNLVM
                     HGQESSDKVLAALQRRVENLRSLGSVLGLLQQNPIAFLRAGTRLDEATIASLIE"
BASE COUNT         1815 a         3364 c         3550 g         1819 t
ORIGIN      
        1 gcgcgggccg cgcagggtct tgtgggtggt cgaggtcacg acgtcggcat gtggcaccgg
       61 gttggggtag acgcccgcgg cgatgaggcc cgcgtagtgc gccatatcaa ccataaatat
      121 cgcgccgatt tccttggcga ccttggcgaa gcgctcgaag tcgatgcgca gcgcataggc
      181 cgaggccccc gcgatgatca gcttgggctt ggactcgtgc gccttgcgct ccatcgcgtc
      241 gtaatcgatc tcttccttgt cgttcaggcc gtaggaaacg acgttgaacc atttgccgct
      301 tatattaaga gccattccgt gcgtcaggtg gcccccttcg gccaggctca tgcccatgat
      361 cgtgtcgccg ggcttcagga aagcaaggaa caccgcttca ttggcttgcg cgcccgagtg
      421 cgcctgcacg ttggcggcgt cggcgccgaa cagctgcttg acccgggcga gcgccagttg
      481 ttcggcgacg tcgacgttct cgcagccgcc gtaatagcgc ttgcccggat agccctccgc
      541 gtacttgttg gtcagctggg ttccctgcgc ggccatcacg gccggcgagg cgtagttttc
      601 gctggcgatc agctcgatat gctcttcctg gcgcctgttt tccgcctgga tcgcggacca
      661 gagttcggga tcggtttgtt cgacgaggat gtttcggtgg tacatggcag tccttttaga
      721 cgtggtccct gtgtaaaaac aagggctgcc caggcgaacg gctgaacgcc gggatgggcc
      781 cggcggtacg ctccccagtg gtttcgaagg gatccgccca aacggacccc gcgctgtgtc
      841 cacgtcagcg ccggccgccc gcaagggcgc cgcgcctatc gccagtcgcg tacagcctta
      901 gtgtaccgga tgggcccggg tgtggctcag gacagcgaga cggccgcgac cttctcctcg
      961 ggggcctgcg gctgggggct gatgggcgcg atcgtcctca gaacgggcgg gtgcagcggg
     1021 acgccgcggc cggcggccac atgcttcagc cgcgcgtgct cggccaggac cttgtcggca
     1081 tagccaccgt cgctgtccag gttggccgca cccacgtaga acttgagccc cgcttgcagc
     1141 gaaccggcgc gctggatgct ctcttgaagc accctcacgc caacgcgcag attcgtgacc
     1201 gggtcgaacg cggcgagttt cccgccgaaa ttctcgtact tgtcgctgtg cacgccggtc
     1261 atgacttgca tcagcccctg ggcgccgacg gagctttggg cgaatgggtt gaatccggat
     1321 tcgaccgcca tgatggcgag gatcagggtg gggtcgagct tggcacgcgc gcccagttcg
     1381 taggcctcgg cgaccagggc gcccaggggc tccggggcca cccggtattt cctgctgagc
     1441 cagtaagcca ccgcggcttg ctgcttgggc agatgttgcg ggttcaccgc cgtggcacga
     1501 tcgatcgcgc cgagctcaat gcgcatgccg acggcagcca tctgccgatc atgcagccag
     1561 ctcctgagct tgatttcgcc ggcatcgcgc aggtcgggcc gggccatgag gccgaccaga
     1621 ctgaacacca cggccaggcc gacgagggca aagctgttat gcgtaatttc gaaaaagccc
     1681 tgggcgatgt cggaggcgaa ggttcgcagg ccctgggctg ttcttgctaa cgctgtcata
     1741 gcacttcctt tctacgcgcg ggcctgcttc gtggtgctgc tcagggcagc atcacaccgt
     1801 caggctcctc gtttggattg gtacgccatc aaattgccgg gagtcctgcg ggactcgtcg
     1861 cagcgattcg acttagccgg ggtcggcagc ggattcgggt tgtcccagtt cgttgctgag
     1921 acgggcgaat tctaggttcc cggctaatac gaagtcaaga atatcaagtt acgttcttat
     1981 tcgcaattaa cctatatacg gcttaattta cgtcctgggc ggtactattt cccgtcagcc
     2041 aaagggtagt cgcgatgtcg gactaagccg gcgagcgttc cttcccaccc cgcaatgcta
     2101 taaaatagat agcgtaatcg caataactgg aaaggccaag cggctgaaac atgggcgggc
     2161 atcgcgatcg cgctgccgct ccgtgtttcc atattgtgga aaatgggccg taattcaccg
     2221 cttatcggaa aatcccgtcg taacgagatt ggtgcggttc tctaacctgt tgaggcgcgc
     2281 gccttttaac ccaggaccgg gcgatcttgt caaggcaata tcggcaatca tggcggcggc
     2341 ggccattgaa aactcggact tgaaaagagc tggacactga cctaacttca aagggcctga
     2401 gtaatcgggc atttagccgg tagggccact tcggtggtag ccccggcccc tcttggaggc
     2461 aatctattgc ctccggcgca tccggggacc aatactccct ggttgcgaac ccaggcggcg
     2521 aaagacttta agtcagccgt caagcccggc aggcggtttc gccgtctgtc cgggctttct
     2581 cttttgcgct tgcttttttc gctttctcat cgtttgttcg cgcacttcgg cgctgccttg
     2641 tgtgagccgt ggttaccgcc cgcgatgccg tgcctcgttt cgcggcgaga atagcggttt
     2701 gccctccccg gcagggcttt cgtccaacga tcccagaacc tccagtgact caagccaccc
     2761 tcaaaaccac cgacacccgt tcgctcgaca agatgaccgg cggcgcgttc tccgctccta
     2821 cttccggcga gcgttccgcg cgagtgcgcg aatggctggc tgccaatccc agcgtggaac
     2881 agatgcagga ggtattccgc gaactcagcg ggcgtgacaa gggcgcggcc aagctgctgc
     2941 gcgaaaagct cgacgagatc aagcgcagca agggccagga agccatcgcc gcggaatggg
     3001 ccgaaaaagc ccgcacgctg ctggctcagg acaaggtgaa tatcgccgac gcgatggcgt
     3061 ggcagcgcga cgccgccaag gccggtgcgc cgctgagccg cgagccgctg gcttcactga
     3121 aggcgcagct ggccgagagg atcaagggaa tcgaggacct gcagcaccgc gtccaggttc
     3181 agcgcgaggc tgccgtgttg ctggcccagc ggatcgaggt gctttccacc aagtcctggc
     3241 gcgacgccca ggcggcggcc gaggccctcg ccggcgatgt tccggattgg caggcacagg
     3301 cccaggcatt gcttgcggat cccaattggc agagcgtgga caacaagttt ccacccttgc
     3361 tcgaggcatc ccgcacccag ctgcaggcgg tgtgggacgc tttccagagt gcgctggcgc
     3421 aagcggtggc cgcggccgag gacgcttccg cgccgctgcc acccgttccc gtatgggccg
     3481 acgaactgcg cgccgtgcgc ggtgtcgcgg tcgaagcaac ggccaggccg gccaaaccca
     3541 agatcgaccc cgaggttcgg gcccaagcca cccaggccgt gcgcgaggcc cttgccaagc
     3601 tggagcagga gatcgcgcaa ggccatggca aggccagtgc cggtgccgcc gcggccctgc
     3661 gcaacgcgct caaggaacac ggcaagctga tcgacgacaa gctcgagaac caggcccatg
     3721 cggcgctcgc ggcggcgggg gaactggaag gctggcagcg ctggcgcgcc gaccagctgc
     3781 gccaggaact ggtggccaag gccgaaggcc tgttcgagca ttcggcaccg gtggagggcc
     3841 aggagccgca gccgcccaag ccccgctatg gcgggcgcaa gatgcaggaa cagcttcggg
     3901 cgctgcgcga gcaatggaag cagaccgacc agggcggcgc tcccaaccat gccctgtgga
     3961 aacgcttcga tgacgcttgc aacgaggccc acaaggtggt ggaggcctgg ctcgacaagg
     4021 tcaaggccga cgcggccgaa caccgggccc agcggctggc gctgatcgat gaggtcaagg
     4081 cctgggccgc cgagaacctg actgccaggg atggcgactg gaagggattc aaccgcatcc
     4141 tgcaccagtt cgaaggccgc tggcgcgacg ccgggcacct gagcgaaaag gcctttgccg
     4201 aattgcagcc cctgtggaag gaggccatcc aggccgccgc cgctcccttg gaagcgatcc
     4261 agaaggaaag cctggaacgg cgccacgcca tgatcgacga ggccaaggca ctcggcgctg
     4321 cgccgcagtt gcgggtcgac gccgtgaagg ccctgcagca gcactggcag gccgaggccc
     4381 aggccgttcc gctggaccgc aagcacgagc aaaagctctg ggatgctttc cgcaagccga
     4441 tcgacgacgc cttcaaccgc aagggtgaag aacgcgagaa ggcggccgcg gcgctgagcg
     4501 agcgcgaccg caccgtgctc gagtcttcca aggcactcga ggccgcgaat gcgaccggcg
     4561 acgtgcagaa gatcaaggcg gcgatggcgg cgctcgatgc ggcgctgcat ggccaggcgc
     4621 aggcggccgc ggccgtcgcg gcggcagggc cggcgctggt ggcgccgccc gccgaagcac
     4681 ccgttgccga ggccgcttcc gccgacgaag ccgcggccgg tcccgccaca gacgaggcgc
     4741 cggccgaggg cgaaggcgaa gcggctcgcg agcccgccgc gccggccaag ccggcgccca
     4801 agcccgtcat cgcgatgcgt ggcgacgacc ggccgggcat gaagaaggcc gaaccgatgc
     4861 cggctgggcg gggcggcaag ttcggcgacc gcaagggcgg cccgggcggc agattcggcg
     4921 agcgccgcga tgaacgctcc ggggcgggtg gcgggggccg tttcggtgac cggccggcgt
     4981 tcgaagaccg cgggccgcgg ctgggcgacg tggctttccg tgcccagcgt gaagccctgg
     5041 aacatgcaca gctgaccctc aagaagctcg cggcgcaggc ccatggcgaa gcgctgaccc
     5101 agctcatgac ggcctgggag cagcgcgcgg ccgaccaggt gccgagccag caggacttgg
     5161 gcaaggcggt ggcgccggcg gtgcgcagca gctgggtgca ggcattgggt gccgcgcctt
     5221 ccggcgatgg ggccgaggcg ttgttgcggc tggagatagc agccgaagcg ccgacgccgg
     5281 ctgagcacct gagtgcgcgg cgggcgctgc agctgcaact gctgacgcgc cgcaacgatc
     5341 catcgccggc gcagacctgg ggacaggacg ccgcgcgtgt gctggcgagc gcttacgaag
     5401 caggcgctgc caggcgcctg caaggcgttc tcaagacgct gctgcgccgc taggccggcc
     5461 gaaaaaaact gtccagcagc ggcaccagcg ccgggaactc ggcctcgaac cgctgccggt
     5521 tgacgaagta ggcttcgcaa gccacagcga agaattcgtc gatcccggtc gccccgtagg
     5581 tgtccagcca ggtctcctcg ccgccgaatc gttcggcgat gatgaccttc tcgcgaaacg
     5641 cctggtactc ggcctgcatc acggccagcc agctctcgcg tgccgcccgc gtgggcaagg
     5701 gcgggcagcc gtcgggcgca ccgtcgcgca tatctatctt gtggatgaac tcgtggatca
     5761 ccacgttgta gccttgttcg gcggtttttc ccgcgctgtc cacatcgctc cagttcagca
     5821 tgacgggacc accctccatc gcttcgccgg ccagcacctc gttgtaatga tggaccacgc
     5881 cgagctcatc ggtcacggtt cggcgcgcga gcacttcccc cgggtgcacg acgatgccca
     5941 cgaagtcgtc gtaccaggcc aggccgaggt tcagcacggg cagcacggct tgcgctgcga
     6001 tggccaatgc aacggcatcc gtgatcacca ggccgttggc gccatggaat tccttggtga
     6061 ccaggaactg cgcggccagc tccctcagcc gcggcagttc ggccgccggg cgcgacgcga
     6121 ggaaaggata acgggccagc aggtcccgcc acaggctgtc cggaatgtca cgcgcggcgc
     6181 ggcgttttcg caaccagctg aacatcagga gagtgcgatg cgcttggcgc cggccgcggt
     6241 cagccgcagg gcctgcaagc gcggcggctt gcttcccgcg tcccagtcgc tgagcacgat
     6301 gcgccggcgc tcgcccaagg cgtggtcggc gggcctatgc gtgtggccgt ggatcagcac
     6361 ctgggcgtcg gccgcatcca gccatgcggc ggccgccgcg ctgtcgacgt cggggtactc
     6421 gacgcccgat cgcttgcggg cttcgctttg ttcacgaagc gcccgggcaa tggcttggcg
     6481 ctgcgcgagg gttttcgaca agaactccat ctgccaggac ggcgcgcgca cctgcgaacg
     6541 gaagcgcatg taatcggtgt ccgccaggca caaggcgtcg ccatgcgtga gcagccagcg
     6601 ccggcccgcg aaacccagca ccgtcgggtc gggcagcagg gtggtgcggc aggctttcag
     6661 gaatccctcg cccaccagga agtcgcggtt gccgtgcatg aagaacacgg ccatgcgccc
     6721 ggccgccgcc tgcagcaccg aggcgcaatc ggcggcgaag ccgggttcgc ccgccgtgtc
     6781 gtcgccgacc cagacctcga acaggtcgcc caggatgaag acggcgtcgg ccggtgtttc
     6841 gcgcatgtag cggcgccagg cctcgaaagt ggcgggatcg gtcgcgtgca ggtgcatgtc
     6901 ggaaatgaca tccgccgtgt gccacgaagc cggcccctgc agctccgtga tcgggggcac
     6961 ggcgacggtg gacatccgcg atccgcgatc ggtcagagcg cgacggcctt ctcgatcacc
     7021 acgtcctcgt tcggcacgtc gtcgtggaag cccttgcgcc cggtcttcac gcccttgatg
     7081 cggtccacga cgtcggtgcc gccgacgacc ttgccgaaca ccgtgtagcc ccagccttgc
     7141 gcgctgggtg ccgtgtggtt gaggaattcg ttgtcggcca cgttgatgaa gaactgcgcc
     7201 gtggcggaat gcgggtcctg cgtgcgcgcc atcgcgaccg tgtagttccg gttcttcagt
     7261 ccgttgttgg cctcgttctc gaccggcgcg tcggtgggct tttgcttcat gccgggctcg
     7321 aagccgccgc cctgcaccat gaagccgggg atcacgcgat ggaagatcgt attgtcgtaa
     7381 tggcccttgt tgacgtaggc gaggaagttc gcgaccgatt tgggcgcctt gtcctggtcc
     7441 agctcgaggg tgatgacgcc gtagccggcg atgtgaagtt cgactttggg gttgctcatt
     7501 ggggtctcct aatcagttga ggacagagct gaaggtctgt cccgcaaggt ttcacgcaag
     7561 ttggggacgc agctgaaggt ctgtcacgga aggttcattt aaccaaggtt gccgatgtga
     7621 tgacgaccgg cgtcttcggc acgtcgctgc cgaacgggcc ggccgccccg gtgggcatgg
     7681 ccttgatctt gttgaccacg tccatgccgc tcacgacctt gccgaatacc gtgtagccgt
     7741 aaagctcctg ggcggcggcc agtcgtgctc gcggctcgtt ccgataaacg cgtcccttgt
     7801 attcgaaccg cggcaccggg tcgcccggcg ggatgacgac ggggtcgagc gacgcgttgt
     7861 ccttgacgtt gatgaagaac tgcgaggagg ctgaattcgg gtcgcttgtt cgtgccatgg
     7921 acagcgtgcc gaccacattc ctcggcccgc ccttggcgag cgcttgctgc ccttcatggg
     7981 caacgggggg ccgggtcggc ttttgaatgt agcgggaatc gaagccgccc ccctggatca
     8041 tgaagttgtc gatcacgcgg tggaagatcg tgccgtcgta gtgcttgtca ttcacgtatt
     8101 gcaggaagtt ctccacggtc ttgggtgcct tgtcgggata aacctcgacc acgatgtcgc
     8161 cggcggtcgt ggcgaacttc accttgggcg cttcctgcgc ggcgacgagc cccgtggccg
     8221 tcgccaggcc cagggacagg gcgagggccg ccaagcgagg ggtcatgaca gtcatcatct
     8281 actgattact ccttcaagaa acgtatcgat tgttgttggc gggccgaatg tccgtgcgat
     8341 gcgcggcggg cgaccctcgt ttggggctgc ccgggggcgt gaaaagaccc aatggtaacg
     8401 gcggtggcgg ccccgtgggc ctgctcagcc accctcggat gcgttgcgct ttgcgtcggt
     8461 tcccggcgag ttcggtcccc gcgccgccgg ccgggcggcg tccttggcgg ccggcgcgag
     8521 cagttggcgg atcagcgcca gcttgggccg cacactcgta ttgcgggcat cgagttgctg
     8581 ggcccggcca taggcctggc tcgccagctg ggcatagatg tcgccaaggt tttcgtgtgc
     8641 cgtcgcatac ccggggttgg cgcgaatggc cgtttccagc gcggcgcggg ccttgtcgaa
     8701 ctcgctttga cccgcgtaca gcacggcaag attgttgtag ggctcggcca gctcggggta
     8761 ttcctgcgtc aggccggtga atgtggcgat cgcctctgcc gtgcggccgg attcggactg
     8821 gaccacgccc ttgaggaatc gcatctgcgg gtcgcgcggc cgggccgcga ggtactggtc
     8881 cgccttgacc agcgcttcgg ccagtttgcc cgatcgcatc agctgggtca cgtcgccgaa
     8941 atcgtcggcg gcgccggcca gcggcaacca caacgcggcg gcgagcacca gcatgaaaaa
     9001 tccacgggga agggcgctgc cgccatgcgt cataaggcct cgatgaaaat cttgcgctga
     9061 caagcccgga cggagggcta tatactgcgc tggattgtag ccgcggcact gcgcgcgcag
     9121 ccccaggcaa gtacccccaa accccttcag atgatccgaa ccagcgccga gcgacgacgt
     9181 accgctcaga gacgcccccg acatcaccca tgagcctgcg catctacaac acgctgtcgc
     9241 gtgccttgga ggatttttcc ccgctcgagc ccggccatgt gcgcatgtat gtttgcggca
     9301 tcaccgtcta cgacctgtgc catgtcggcc acgcccgcgc caacgtggcg ttcgacgtgg
     9361 tgcagcgctg gttgaaggcc agcggctaca agctgaactt cgttcgcaac atcaccgata
     9421 tcgaggacaa gatcatccgc cgcgccgtgg aaaacggcga gaccgtgcgc tcgctcaccg
     9481 accggatgat cgccgagatg taccgtgatt tcgacgcgct cggcatcgag cggccgacgc
     9541 acgagccgcg cgccaccgac tacgtgcccc agatgctgga catcgtcagg cagctcgagg
     9601 acaaggacct ggcgtatcgc gcggagggcg gggacgtgaa ttacgcggtg cggcgctttc
     9661 ccggctacgg caagctctcc ggcaaaaccc tcgacgaact gcacgcgggc gagcgtgtcg
     9721 cggtgctcga aggcaacaag gaagatccgc tggacttcgt gctctggaaa gccgccaagc
     9781 cgaccgagcc ggacgacgcc cagtgggaca gcccctacgg caagggccgg cccggctggc
     9841 acatcgagtg ctcggcgatg gcctgcgcct tgctgggcaa gaccttcgac atccacggcg
     9901 gcggcgccga cctgcagttc ccgcatcacg agaacgagat cgcgcaaagc gagggcgcca
     9961 acgggcaacc gctcgcgcgc tactggatgc acaacggctt cgtcaacatc gacaacgaga
    10021 agatgtcgaa gtcgctcggg aacttcttca ccatccgcga ggtgctggag aggttcgacg
    10081 ccgaaacctt gcgtttcttc atcgtgcggt cgcactaccg cagcccgctg aactacagcg
    10141 acgttcacct ggatgatgcg cgcgcatcgt tgcgccggct gtacaacgta ttggccgacg
    10201 gcgatgctct cttgcattcg cgcgggttaa aagcgtcggt tgtcccgttt gcggatttgg
    10261 acaagtctga cgggatggtg agcgccttcg ttcgcgcgat ggacgacgac ttcgggactc
    10321 cgcaagccat atcggtcctg ttcgcactgg ctggtgaaat cgggactgct cgcaacttgg
    10381 tgatgcacgg tcaggaaagt tcggataaag tcctcgcagc gcttcagcga cgcgtcgaga
    10441 acttgcgatc gcttggaagc gttcttggac ttctccagca gaatccgata gcgttcctgc
    10501 gcgccggtac gcggctggac gaggcgacga tcgcaagcct gatcgagc
//
LOCUS       DASWIY010000361        11489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1343984, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000361 DASWIY010000000
VERSION     DASWIY010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11489
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343984"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VGA59_12815"
     CDS             complement(<1..373)
                     /locus_tag="VGA59_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9720602.1"
                     /translation="MAITEISSPIERPRLSDTIYGQLLEEIMAGRFSAGDRLPTENQL
                     AQRFSVSRPVVREALQRLQTDGVVIARQGSGTYVQRSPSQRVAELTSAVSLHEVLQVM
                     EVRMALEELSARLAARNRSEEQ"
     gene            585..1346
                     /locus_tag="VGA59_12820"
     CDS             585..1346
                     /locus_tag="VGA59_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX9720603.1"
                     /translation="MRTHKPVVWLPACHRHLDLADPGGYTVLADRYAGAVTALGLQPV
                     LFPMAGAPDVPDLLPMVDGVLLTGSPSNVDASRFGAAALPDDLLDPRRDALTMALVPA
                     AIAARTPLFGVCRGMQEMNVALGGSLHQQLHSEPGLLDHREPDDEELAVQFAARHEVR
                     LVPGSAFARWAGGTTAQVNSLHGQGIKRLGEGLVAEAFAPDGLVEGVRVAHAQAFAFG
                     VQWHPEWRHETNPFYARTLEAFAQACAGHQRRAAP"
     gene            complement(1567..2382)
                     /locus_tag="VGA59_12825"
     CDS             complement(1567..2382)
                     /locus_tag="VGA59_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX9720604.1"
                     /translation="MLDLRDWPGAERGRIAGVFTDIDDTLTTGGAITADALAALARLR
                     AAGVHVIPITGRPAGWSEPFAMQWPVDAIVAENGALALVRQEEGTNSLVKLYQQDART
                     RAVNSVRMRQAARRIVSEVPGAVLSRDSAGRETDIAIDHAEFVHLPAARVAQVVRLMQ
                     EEGMNATVSSIHINGWYGGHNKLSGARWIVRELFGRELDAEIDRWVYVGDSPNDVLMF
                     EHFPHSVGVANIRRFEAQLTHPPRYIAQGERGAGFAEVARAILAANAESDGRE"
     gene            complement(2391..4028)
                     /locus_tag="VGA59_12830"
     CDS             complement(2391..4028)
                     /locus_tag="VGA59_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX9720605.1"
                     /translation="MADGSIAEQTRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQIAG
                     SVKARKATRHATSDWMEIEKQRGISVASSVMQMLYRDHVINLLDTPGHKDFSEDTYRV
                     LTAVDSALMVIDAANGVEAQTRRLIEVCRQRDTPIITFVNKMDREVREPLDILDEVER
                     ELGMDCVPMTWPVGQGKTFGGIMNLRTQTMTVFESGSERRPQDFETIPLADRETLLER
                     FGHEFETAEQSMELAVGASPSWDHAAFLAGKQTPVFFGSGVNNFGVAEVLEALVDLAP
                     SPGPRTSHLVVNKQMVVRQVHPDDENFSGVVFKVQANMDANHRDRIAFVRMASGKYTP
                     GMKLKVQRTGKELRPTSVVTFLSQRREAVDEAYAGDIIGFTTHGGVQLGDTITDGASL
                     QFTGLPFFAPELFMTVILRNPLRTKQLQQGLAQLGEEGAIQVFRPQVGGPMLLGAVGQ
                     LQFEVVQHRLKSEYDADVRLEGCQYTGARWITADTPAELRAFVDAYPQRMALDAANSL
                     AFLCTSPYDVRLAQERFPHIHFHPLREHAGLALHNVG"
     gene            4277..4618
                     /locus_tag="VGA59_12835"
     CDS             4277..4618
                     /locus_tag="VGA59_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9720606.1"
                     /translation="MTARNKLLAAPPYPVEQTLKRLGANLRTARLRRNLTIEDIAEKI
                     GTGVRAVADAEHGKASTGMAVYAAMLWALDLLGHMDDVAAPEKDEEGQALALSRERAR
                     ARPDRSLDNDF"
     gene            complement(4629..6473)
                     /locus_tag="VGA59_12840"
     CDS             complement(4629..6473)
                     /locus_tag="VGA59_12840"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEX9720607.1"
                     /translation="MPLHLVGETIDKTRSHYLAETGKLVQLMRGIYVDATDDIDQTVL
                     AHAVRIARYLYPNAYLSAASAVLLGPTRDGRLYLTGQRGQRTRIRALEIVQNKAPGKA
                     SLAPAVVADSMGEFSVNVSSIRQRFLEAFRLRSEHAASIDSATREAIAARLVEEYGSP
                     KAASDAVWALARENDWYREGEGAEKFLLRHPSTAPARNEAAFDLTVAWHGVPIGNLVH
                     DGFEWRWSQTDTGTPMPPVIRQTVPGKLPPFIVSLLPEGWLEAVLKDRDERAVLRSGT
                     RYMSNITIAENRSELAALPLDVLATCLADNAAAGVFTGTYAGPGRSAMEQDFERNLAL
                     IYAHADTPRLSGVQIKAPMTLGPDGKLSPSIGTPFTHILKPAGTSGFEALPLVEWLGL
                     ALAREAGLTVPAAALIAMPDGMPPALLVERFDIRTGADDHRLLAMEDFCSVLDSAPDA
                     KYDGTIERVARALKALSSSPDEDLLILVKRALFTWLIADGDMHLKNMAVLKIAGPGDP
                     AFRSVRLAPVYDTLTTRVFPRLEHDRMALKLNGKDERLKRADFLGVAAIAGLRASDTN
                     AAIDDVLQRMQAAVAAITLPKLPDYGPSGEATVNRTLEICRERIVSFA"
     gene            6798..7409
                     /locus_tag="VGA59_12845"
     CDS             6798..7409
                     /locus_tag="VGA59_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720608.1"
                     /translation="MKTAFFLPIALTLFAAVPGLAQPTNQTVFRADNWFVVRSTKPDG
                     AVACTGFYIGQDGVQLSKDNLTVKIPGEVRSVSLRFGDQPVRAPRPLDPTERQMGAVK
                     LAGPDFEQLRRSKTLGLDVATAQGRESHALKLEGLDAALKNIDAGCPLTAAAERAQRA
                     KQQARAKALAAQCAPDAIAKQRAGGVPEWRIAARCPKAAPAKR"
     gene            complement(7399..8478)
                     /locus_tag="VGA59_12850"
     CDS             complement(7399..8478)
                     /locus_tag="VGA59_12850"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX9720609.1"
                     /translation="MPPPTLQPPIEPMLAKIADELPQGDGFLFEPKWDGFRAIVFRTG
                     GEVYIQSRDLRPLNRYFPELERALAQALPKGCVIDGEIVVVNGAGLDFDALQQRLHPA
                     ASRVARLAKETPAAFVAFDLLAAGGRSIMELPLSQRRVRLERLLGQVSRPVYLTPITR
                     DRALAQEWLERFEGAGLDGVIAKVEAGPYLPGKRSMFKIKHARAAECVVGGFRWYRDT
                     QDAVGSLLLGLYDDAGVLHHVGVASAFTLAMRRQLADELGPLRKDALQDHPWRDWDNA
                     NDDAAGRMPGGQSRWSAGKDLSWEAVRPERACEVKYDHLQGMRFRHAAVFLRWRPDKP
                     PLDCRYDQLEVTTDFELAKVFAASA"
     gene            complement(8544..9449)
                     /locus_tag="VGA59_12855"
     CDS             complement(8544..9449)
                     /locus_tag="VGA59_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720610.1"
                     /translation="MTLGPPIEPMWTLTDLVPASPMQPMVPNRGGYTLTMRECAGSEE
                     SLALLESDAAAASEAVGRLHLGWGSFRNLDVMAVRRSDWGLLLDVNFFQFQVWETVRL
                     ALLNSTGAAAFIEAVVPLLPTQPRLRQFSDSTLTWLRGDLERPGSWLFESRPDRYQHI
                     RALFCKGRVAVACADLRSVSPAQGGGWFEELAGRMESARRAGKLVPDTLYVSNIPWML
                     AQPIGFFGESHAPYVREGGAVLDQVRGNLAQVVNSFSWVISAMRLRNDATADNLQWLT
                     QLQQPDDFMAERDWRQLAEIPAAVG"
     gene            complement(9446..10003)
                     /locus_tag="VGA59_12860"
     CDS             complement(9446..10003)
                     /locus_tag="VGA59_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720611.1"
                     /translation="MKARLALLLVTWLIAAAGWAQSGPPGSALVDSREECAALGGSWL
                     ANRSTWQAACQVPWAREECLRLHGAWTAIPLAPNGGACVAQVSQPATARQCTDAGGTW
                     GPPGSSMPFCQPQTAVAAAPVKTASDANKVCAGQGDCIYGCVYNGPPVATGAEVIGRC
                     RRTNKIEGCYSMVEKGRLAGSICKR"
     regulatory      10047..10116
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            10197..>11489
                     /gene="ahcY"
                     /locus_tag="VGA59_12865"
     CDS             10197..>11489
                     /gene="ahcY"
                     /locus_tag="VGA59_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX9720612.1"
                     /translation="MNAVLKPAAPRDCAIADPSLAEWGRKEIRIAETEMPGLMAIREE
                     FAKSQPLKGARITGSLHMTIQTAVLIETLQALGAQVRWASCNIFSTQDHAAAAIAAGG
                     TPVFAVKGESLADYWDYTHRIFEFGPKGADGEGPNMILDDGGDATLLMHLGRRAEKDP
                     SVIANPGSEEETCLFAAIRAKLAQDATWYSRKGAQIVGVTEETTTGVHRLKEMSAKGT
                     LLFRAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVALVCGYGDVGKGSSQ
                     ALRALSAQVWVTEIDPINALQAAMEGFKVVTMEYACDKADIFVTATGNKHVIRHEHMV
                     KMKDQAIVCNIGHFDNEIDIASVEKYPWEEIKPQVDHIIFPDGKKIILLAKGRLVNLG
                     CATGHPSFVMSSSFANQTIAQIELFTHSDYYEVGKVYVL"
BASE COUNT         1976 a         3780 c         3824 g         1909 t
ORIGIN      
        1 cctgctcttc gctgcgatta cgcgcggcca gcctggccga gagttcttcc agcgccatgc
       61 gcacttccat cacctgcagc acttcgtgca ggctgacagc gctggtgagt tcggccacgc
      121 gctgcgaagg gctgcgctgt acataggtgc ccgagccctg gcgcgcgatg acgacgccgt
      181 cggtctgcag gcgctgcaac gcctcgcgca ccaccgggcg cgataccgag aagcgttgcg
      241 cgagctggtt ctcggtgggc aaccggtccc cggccgagaa gcgcccggcc atgatctcct
      301 cgagcagctg gccgtagatc gtgtcggaca gccggggtcg ctcgatgggg gaggaaatct
      361 cggtgatggc catgccgcgg gtggttgctc gcccattcta caaacggccg ggcacgcgcc
      421 gcattctgca cagcgagcga gcgagcgggc gcgggcgggt gagttcgtgg gcggccatat
      481 catggcggcg gccgactcca ccggcttcgc gcccctggtc agcgaaaaag ccctggtcga
      541 gaagctggag ctggtcaaga cgcagtcagc gctatccttg cggcatgcgc acgcacaagc
      601 ccgtggtctg gctgcccgcc tgccaccgcc atctggatct cgccgacccc ggcggctaca
      661 cggtgctcgc ggaccgctat gccggtgccg tcacggcgct cgggctgcaa cccgtgctgt
      721 tcccgatggc cggcgcgccc gatgtgcccg acctgctgcc catggtcgac ggcgtgctgc
      781 tcacgggttc gccgtccaac gtcgacgcgt cgcgcttcgg cgccgcggcc ttgcccgacg
      841 acctgctcga cccgcgccgc gacgcgctga cgatggccct ggtgcctgcg gccattgccg
      901 cgcgcacgcc cctcttcggt gtctgccgcg gcatgcagga aatgaacgtg gcgctgggcg
      961 gcagcctgca ccagcagctg cattccgaac ccggcctgct ggaccaccgc gaacccgatg
     1021 acgaagagct ggcggtccag tttgccgcgc ggcatgaggt gaggctcgtg ccgggcagtg
     1081 cgttcgcgcg atgggccggc ggcaccacgg cccaggtcaa ctcgctgcac ggccagggca
     1141 tcaagcggct gggcgagggc ctggtggctg aagccttcgc gccggacggg ttggtggagg
     1201 gtgtgcgggt ggcccatgcg caggccttcg ccttcggcgt gcaatggcat cccgagtggc
     1261 ggcacgagac caatcccttc tatgcccgca cgctcgaggc gtttgcgcag gcttgcgccg
     1321 ggcaccagcg cagggccgcg ccgtgaagga gagagagaga gagagagaga gagagagatg
     1381 tcatgccgga cttgatccgg catccatgaa ttcctggatt gcggggccgg gccggagcgt
     1441 gatgcctcgg cacgtgcata gcgtaggccc ggcattgggc tcgctgcact ggacagctgc
     1501 gcgccccggg cgttccgcga gcccagcccg caatgacaac cagaaggctc agacgcggcc
     1561 aggcggctac tctctgccgt cactttcggc gtttgccgcc aggatcgccc gcgcgacttc
     1621 cgcgaaaccc gcaccgcgct cgccctgggc gatgtagcgc ggcgggtgcg tcagctgcgc
     1681 ttcgaagcgg cggatgttgg ccacgcccac gctgtgcggg aagtgctcga acatgagcac
     1741 gtcgttgggc gagtcgccca cgtaaaccca acggtcgatc tcggcatcga gttcgcggcc
     1801 gaacagctcg cgcacgatcc agcgcgcgcc cgacagcttg ttgtgaccgc cgtaccagcc
     1861 attgatgtgg atcgagctga cggtggcgtt catgccctct tcctgcatga ggcgaaccac
     1921 ctgggccacg cgcgcggccg gcaggtgcac gaattcggcg tgatcgatgg cgatgtcggt
     1981 ctcgcggccc gcgctgtcgc gcgacagcac ggcgccgggg acctcgctca cgatgcgccg
     2041 cgccgcctgc cgcatgcgaa cggagttgac ggcccgcgtt cgcgcatcct gctggtagag
     2101 cttgacgagg ctgtttgttc cctcttcctg tcgcaccagg gccagcgccc cgttctcggc
     2161 cacgatggcg tcgaccggcc actgcatggc aaaaggctcg ctccagccgg cgggccggcc
     2221 ggtgatggga atgacgtgga cgccggcggc acgcagcctg gcgagtgccg ccagcgcgtc
     2281 ggcggtgatt gcgccgccgg tggtgagcgt gtcgtcgatg tcggtgaaca cgcccgcgat
     2341 gcgaccgcgt tcagcgccgg gccagtcgcg caggtcgagc atcgaatgcc tcatccgacg
     2401 ttgtgcagcg ccagcccggc atgctcgcgc agcggatgga aatggatgtg gggaaagcgc
     2461 tcctgcgcca gccgcacatc gtagggcgag gtgcacagga aggccagcga gttggccgca
     2521 tccagcgcca tccgctgcgg ataggcatcg acgaatgcgc gcagctcggc cggcgtgtcg
     2581 gccgtgatcc agcgcgctcc ggtgtactgg cagccctcca gccggacgtc ggcgtcgtac
     2641 tcggacttga gccggtgctg caccacttcg aactgcagct ggcccacggc gcccagcagc
     2701 atgggcccgc cgacctgcgg tcggaacacc tggatcgcgc cttcctcgcc gagctgggcc
     2761 aggccctgct gcagctgctt ggtgcgcagc gggttcctca ggatcaccgt catgaacagc
     2821 tcgggcgcga agaagggcag tcccgtgaac tggaggctgg cgccgtcggt gatggtgtcg
     2881 cccagctgca cgccgccgtg cgtggtgaag ccgatgatgt cgcccgcgta ggcctcgtcc
     2941 accgcttcac ggcgctgcga caggaaggtg acgacggatg tcggccgcag ttccttgccg
     3001 gtgcgctgca ccttgagctt catgccgggg gtgtacttgc cggaggccat gcgcacgaag
     3061 gcgatgcggt cgcggtgatt ggcgtccatg ttggcctgaa ccttgaacac cacgccggaa
     3121 aagttctcgt cgtccggatg cacttgcctg accaccatct gcttgttgac caccagatgg
     3181 ctggtgcgcg gccccggcga aggcgccagg tcgaccagcg cttccagcac ctcagccacg
     3241 ccgaaattgt tgacgccgga gccgaagaac acaggcgtct gcttgcccgc cagaaaggcc
     3301 gcgtggtccc aggacggcga ggcgccaacg gcgagttcca tgctttgctc ggcggtttcg
     3361 aattcgtggc cgaagcgctc gagcagggtt tcgcggtccg ccagcggaat ggtttcgaag
     3421 tcctgcggcc ggcgctcgga gccggattcg aacacggtca tcgtctgcgt gcgcaggttc
     3481 atgatgccgc cgaaggtctt gccctggccg accggccagg tcatgggcac gcagtccatg
     3541 cccagttcgc gctccacctc gtcgaggatg tccagcggct cgcgcacctc ccggtccatc
     3601 ttgttgacga acgtgatgat gggcgtgtcg cgctggcggc agacctcgat cagccggcgc
     3661 gtctgcgctt ccacgccgtt ggccgcgtcg atcaccatca gcgccgagtc cacggccgtg
     3721 agcacgcgat aggtgtcctc ggagaagtcc ttgtggcccg gcgtgtccag caggttgatc
     3781 acatggtcgc ggtacagcat ctgcatcacc gagctggcca cggaaatgcc gcgctgcttt
     3841 tcgatttcca tccagtccga ggtggcgtgg cgcgtggcct tgcgcgcctt gaccgaaccg
     3901 gcgatctgga tcgcgccgga aaacagcagc agcttttccg tcagcgtggt cttgcccgcg
     3961 tcggggtggg agatgatggc aaacgttctt ctccgccttg tctgctctgc gattgaaccg
     4021 tctgccatca gaactctttc cccgttatgt acacagctat gtacgcattc cccaatgtgc
     4081 tcatagctcg tgccacatca gtctctttgt cgcccttggc agccggtcag tgctgcccgc
     4141 gactggattt tagcacgcaa acgctcttga gagcgtctta tatccattag aatgctcgtg
     4201 agagcgtgta tgccgggtca ttgcccgcca agcatggtat ctgttggggg acgtgccaat
     4261 ttttcatcgg tgaaccatga ctgcccggaa caagctgctg gctgcgccgc cttatcccgt
     4321 cgaacagacg ctcaagcgcc tgggcgcgaa ccttcgcacg gcacgcctgc gccgaaatct
     4381 caccatcgaa gatatagccg agaagatcgg cacaggggtt cgtgcggttg ccgatgccga
     4441 acacggcaag gcctcgacgg ggatggctgt ctatgcggcg atgctgtggg cgctcgatct
     4501 cctgggccac atggacgacg ttgccgcgcc ggaaaaagac gaggaagggc aagccctcgc
     4561 cttgtcccgc gaacgcgccc gcgcgcggcc tgaccgcagc ttggacaacg atttctagtc
     4621 cctcctgctc acgcgaacga gacaatccgc tcccgacata tctcaagcgt tcgattcaca
     4681 gtagcctcac cagatggccc gtagtcgggg agcttgggta aggtaatcgc ggcaacggca
     4741 gcctgcatcc gttgcagaac gtcatcgatc gcggcattcg tgtccgacgc ccgaagtccg
     4801 gctatcgcgg cgaccccgag gaagtcggca cgcttgagcc gttcgtcctt gccgttgagc
     4861 ttgagcgcca tgcggtcatg ttcaaggcgg ggaaagaccc gtgtcgtcag cgtgtcgtag
     4921 accggcgcaa gccgaacgct gcggaaggcc ggatcgccgg gtccggcgat cttcagaacg
     4981 gccatgttct tgagatgcat gtcgccatcg gcgataagcc aggtaaacaa ggcgcgcttc
     5041 acgagaatca gaaggtcttc atcgggtgat gaggacagag ctttcaaggc acgcgccacg
     5101 cgttcaatgg tgccgtcgta cttggcatcc ggggccgagt cgagcactga gcaaaagtct
     5161 tccatcgcca gcaggcggtg gtcatcggct cccgtgcgaa tatcgaaccg ctcgaccagg
     5221 agcgccggag gcatgccatc aggcatcgcg atcagggccg ctgcgggaac cgtgagcccg
     5281 gcctcccttg ccagcgcgag gccgagccac tcgaccagcg gcaaggcttc gaagccgctg
     5341 gtccctgcgg gtttcaggat gtgcgtgaac ggtgtgccga tgctgggcga cagcttgcca
     5401 tccggcccca gcgtcatagg agccttgatc tgtacgccgg acagacgcgg tgtgtcggcg
     5461 tgcgcgtaaa tcagagcgag gttgcgctcg aagtcctgct ccatcgcgct gcggcctggc
     5521 ccggcatagg tgccggtgaa cacgcctgcc gccgcattgt cggcaaggca cgtcgcgagc
     5581 acatcgagcg gcagggcggc aagctcgctc ctgttctcgg caatggtgat gttggacatg
     5641 tagcgcgtgc ccgagcgcaa cacggcccgc tcatcgcgat ccttgaggac cgcttcgagc
     5701 caaccctcgg gcagcaacga gacgatgaag ggcggcagct ttcccggtac cgtctggcga
     5761 ataacgggcg gcataggcgt accggtgtcg gtctgggacc accgccattc gaagccatcg
     5821 tgaacgaggt tgccaatcgg caccccatgc cacgcgaccg tcaggtcgaa cgcggcctcg
     5881 ttacgggctg gcgctgtcga aggatggcgc agcaagaact tctcggcccc ctcgccctcg
     5941 cggtaccagt cgttctctcg ggcgagcgcc cagacggcat cgcttgcggc cttggggctt
     6001 ccgtactcct cgacaaggcg ggcagcgatg gcctcgcgtg tcgctgaatc gatggacgca
     6061 gcatgttcgc tgcgcaggcg aaaggcttcg agaaagcgtt ggcggatcga ggacacattg
     6121 acggaaaact cgcccatgct gtcggcaaca acggcgggcg cgagagaagc cttcccgggt
     6181 gccttgttct gaacgatctc tagcgctctg atccgggtgc gctggccgcg ttggcccgtc
     6241 aggtagaggc gaccatcgcg ggtcggcccc agcagaactg cgctcgcggc cgacaggtaa
     6301 gcattcggat agaggtagcg tgcgatgcgc acggcatgag cgaggacggt ctggtcgatg
     6361 tcatcggtgg catcgacata gatgccccgc atcaactgga cgagcttgcc cgtctctgcc
     6421 aggtagtggc tgcgggtctt gtcgatggtc tcgccgacga ggtgaagggg catttttgta
     6481 actttcgcgt gtttcagaca cgtgaaagtt agcatccgta ccgtcggaag gcaagaaaat
     6541 tctgactttt ccgtatttca gaaacgcgaa agttaggctc tcagccgatc ggcacacccc
     6601 agacgtacgc aaaaaccgac aaaccggcga gccttccggt gaaaggccga gacgattaca
     6661 tggccgcgct ggaaaaaggg agtgtggaag gcaacatcgt tccttttgcc caatttccag
     6721 ggagtcttct gaaaggctgg cgctgggcgg gtcggttgac gattgcacgg gtcggacgcg
     6781 gctatagtcg agcggccatg aaaaccgcct ttttccttcc catcgcgctg accctttttg
     6841 ccgccgtgcc cggcctggcc cagccgacca accagacggt attccgcgcc gacaactggt
     6901 tcgtcgtgcg ctcgaccaaa ccggacggag ccgtggcttg cacgggcttt tatattggcc
     6961 aggatggcgt gcagctgagc aaggacaacc tgaccgtcaa gatccccggc gaggtgaggt
     7021 cggtcagcct gcgttttggc gaccagccgg tgcgcgcgcc gcggccgctg gaccccaccg
     7081 agcggcaaat gggcgctgtc aaactggcgg gccccgactt cgaacagctg cgacgcagca
     7141 agacgctggg cctggacgtt gccaccgcgc agggccgcga aagccatgca ttgaagctcg
     7201 agggactgga tgccgcgctg aagaacatcg acgccggctg tcctctcacg gcggctgccg
     7261 agcgtgccca gcgcgcgaag cagcaggcgc gggcgaaagc cctggcggcc caatgcgccc
     7321 ccgacgcgat tgcgaaacag cgcgccggcg gtgtccccga atggcggatc gccgccagat
     7381 gccccaaagc cgcgccggct aagcgctagc cgcgaaaacc tttgccagct cgaaatcggt
     7441 cgtgacctcg agctggtcat agcggcaatc caggggcggc ttgtccggcc gccagcgcag
     7501 gaacaccgcg gcatggcgga accgcatgcc ttgcaggtgg tcgtatttca cttcgcaggc
     7561 ccgctcggga cggaccgcct cccacgacag gtccttgccc gcgctccagc ggctctgccc
     7621 gccgggcatg cggccggcgg cgtcgtcgtt ggcattgtcc cagtcgcgcc agggatggtc
     7681 ctgcagggcg tccttgcgca gcggcccgag ttcgtcggcg agctggcggc gcatcgccaa
     7741 agtgaaggcg gaggcgacac ccacatggtg cagcacgccg gcgtcgtcgt acaggcccag
     7801 caacagggag ccgaccgcat cctgcgtatc ccggtaccac cggaaccctc cgaccacgca
     7861 ctcggccgcg cgcgcatgct tgatcttgaa catgctgcgc ttgcccggca ggtaggggcc
     7921 ggcctccacc ttggcgatga cgccgtcgag gccggcgcct tcgaaccgct ccagccactc
     7981 ctgggccagg gcgcgatccc gggtgatggg cgtcaggtac acgggcctgg acacctggcc
     8041 cagcagccgc tccagccgga cccgccgctg tgacagcggc agttccatga tcgagcggcc
     8101 gcccgccgcc agcaggtcga acgcgacgaa ggcagccggc gtttccttcg ccagccgcgc
     8161 gacgcgcgag gccgcgggat gcaaacgctg ctgcagggcg tcgaagtcga ggccggcgcc
     8221 gttgaccacc acgatttcgc cgtcgatcac gcaccccttg ggcagtgctt gagccagcgc
     8281 gcgttcgagt tcgggaaagt aacggttgag cggccgcagg tcgcggctct ggatatagac
     8341 ctcgccgccg gtgcggaaca cgatcgcacg gaagccgtcc cacttgggtt cgaacaggaa
     8401 accgtcgccc tgcggaagct catccgcgat cttggcgagc atgggttcga tgggcggctg
     8461 gagggtgggc ggaggcaccg gcttattttg cggcgccggc gctgcgcgcg ggggctccgg
     8521 gggcgcgcag gttgtaggac gggtcagcca accgctgcgg gaatttccgc caactgccgc
     8581 cagtcgcgct cggccatgaa gtcatccggc tgctggagct gcgtgagcca ttgcaggtta
     8641 tccgccgtcg cgtcgttgcg cagacgcatg gcggagatga cccaactgaa cgaattgacg
     8701 acctgcgcca ggttgccgcg cacctggtcc agcaccgcgc ctccttcccg cacgtagggt
     8761 gcgtggctct caccgaagaa gccgatgggc tgcgccagca tccacgggat attcgacacg
     8821 tagagggtgt cgggcaccag cttgcctgcg cgccgcgccg actccatgcg gcccgccagt
     8881 tcttcgaacc agccgccacc ttgcgcgggc gacacgctgc gcaagtccgc gcaggcgaca
     8941 gccacgcggc ctttgcaaaa gagggccctg atgtgctggt agcggtccgg ccgggactcg
     9001 aacagccagc ttcctggccg ctcgaggtcg ccgcgcagcc acgtcagcgt ggagtcggag
     9061 aactggcgca ggcggggttg cgtgggcagc agcggcacca cggcctcgat gaacgctgcg
     9121 gcgccggtcg aattgagcag ggcgagccgc accgtctccc agacctgaaa ctgaaaaaag
     9181 ttgacatcca gcagcaggcc ccaatcgctg cggcgtacgg ccatgacatc gaggttgcgg
     9241 aaactgcccc aacccagatg cagccgcccc acagcctcac tggcagccgc tgcatcgctt
     9301 tccagcagcg ccaggctttc ctccgacccg gcgcattccc gcatggtcaa ggtataacca
     9361 ccgcggttgg gaaccatggg ctgcatggga gacgcgggaa ccaggtcggt gagtgtccac
     9421 atcggctcga tcggcggccc caacgtcatc gcttgcagat gctcccggca agtcgcccct
     9481 tctcgaccat ggagtagcag ccttcgatct tgttggtccg ccggcaacgc ccgatgactt
     9541 ccgcgccggt agccaccggc gggccgttgt agacgcagcc gtaaatgcag tcgccctggc
     9601 cggcgcaaac cttgttcgcg tccgacgcgg tcttgaccgg cgcggcggct accgccgtct
     9661 gcggctggca aaacggcatt gacgatcccg gcggtcccca cgtgccaccg gcatcggtgc
     9721 actggcgggc ggtggccggc tgcgaaacct gcgccacaca ggcgcccccg ttcggcgcca
     9781 gcgggatcgc cgtccacgcg ccgtgcagcc gcaggcattc ctcgcgcgcc cacggcacct
     9841 ggcatgccgc ctgccaggtg ctccggttgg ccagccagga gccgcccagc gccgcacatt
     9901 cctcgcgcga gtcgaccagc gcggagccgg ggggaccgga ctgcgcccat ccggccgccg
     9961 cgatgagcca tgtgaccagc aggagtgcca agcgtgcctt catgcccgca gtctagcgcg
    10021 ggcaaggcgc gcttggtaga atgcggtcaa ccgaggagcg ttgcagcgcc agtcaggcgt
    10081 gaggctcgga aaccatcgca acgacgctca cccactgttc atgtggggtg agctcttcca
    10141 tgaggcatcg ccgccaccgc gcagtggttc tcgaaacaca tgtttttgga gccgtcatga
    10201 atgccgttct caagcccgca gcccctcgcg actgcgccat tgccgacccg tcgctggccg
    10261 agtggggccg caaggaaatc cggatcgccg aaaccgaaat gcccggcctg atggccatcc
    10321 gcgaggaatt cgcgaagagc cagccgctga agggtgcccg catcaccggc tcgctgcaca
    10381 tgaccatcca gaccgcggtg ctgatcgaga cgctgcaggc gctgggcgcg caagtgcgct
    10441 gggcctcgtg caacatcttc tcgacccagg atcatgcggc ggccgccatc gccgccggcg
    10501 gcacaccggt gtttgccgtc aagggcgaga gcctggccga ctactgggac tacacccatc
    10561 gcatcttcga gttcggcccc aagggtgccg acggtgaagg cccgaacatg atcctggacg
    10621 acggcggcga tgccacgctg ctgatgcacc tgggccggcg cgcggagaag gacccgtccg
    10681 tcatcgccaa cccggggagc gaggaggaaa cctgcctgtt cgccgccatc agggccaagc
    10741 tggcgcagga cgccacctgg tacagccgca agggcgcgca gatcgtcggc gtcaccgaag
    10801 agaccaccac cggggtgcac cgcctgaagg aaatgagcgc gaagggcacg ctgctgttcc
    10861 gcgcgatcaa cgtcaacgac tcggtcacca agagcaagtt cgacaacctg tacggttgcc
    10921 gcgaatcgct ggtggacggc atcaagcgcg ccaccgacgt gatgatcgcc ggcaaggtgg
    10981 ccctcgtctg cggttacggc gacgtgggca agggctcgtc gcaggcgctg cgcgcgctgt
    11041 cggcccaggt gtgggtgacc gagatcgacc cgatcaatgc gttgcaggcg gcaatggagg
    11101 gcttcaaggt cgtcaccatg gaatacgcct gcgacaaggc cgacatcttc gtgacggcca
    11161 ccggcaacaa gcatgtgatc cggcacgagc acatggtcaa gatgaaggac caggccatcg
    11221 tctgcaacat cgggcacttc gacaacgaga tcgacatcgc ctcggtcgag aagtacccct
    11281 gggaagagat caagccgcag gtggaccaca tcatcttccc ggacggcaag aagatcatcc
    11341 tgctggcgaa gggccggctg gtgaacctgg gctgcgccac cggccatccc agcttcgtga
    11401 tgagttcctc gttcgccaac cagacgatcg cgcagatcga gctgttcacc cacagcgatt
    11461 actacgaggt gggcaaggtc tatgtgctg
//
LOCUS       DASWIY010000362        13831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1344289, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000362 DASWIY010000000
VERSION     DASWIY010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 13831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13831
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344289"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VGA59_12870"
     CDS             <1..201
                     /locus_tag="VGA59_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEX9720613.1"
                     /translation="LADLRNQLAGAVLMGFGGVTALGCTIGQGLAGVSTLAIGSLIAV
                     AGIAAGCIGTLKIILWQSQREG"
     gene            complement(489..929)
                     /locus_tag="VGA59_12875"
     CDS             complement(489..929)
                     /locus_tag="VGA59_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720614.1"
                     /translation="MDDSLVPGSACCDTDHACVFAKALLARAATCELSQRRAIAERDL
                     LECTSPVARVNCGTLAALLHERARFALRLPAPGHPLIHAQSLRLQCGGLSALRRELAV
                     ADSDVHRMVRTAHERHGSLTELPWTAIVAAIAAWQPRRRARTGR"
     gene            complement(934..1143)
                     /locus_tag="VGA59_12880"
     CDS             complement(934..1143)
                     /locus_tag="VGA59_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720615.1"
                     /translation="MPYYVYAIKPMAQLEKLAEFASFKEASMQAKALRAEGPAARIKV
                     IFADNPLAAEDLMLQVREAPPDGDD"
     gene            complement(1145..1513)
                     /locus_tag="VGA59_12885"
     CDS             complement(1145..1513)
                     /locus_tag="VGA59_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEX9720616.1"
                     /translation="MSCTCGAPPAPADPHDDGGCSAGESFALRVLGDDMAPEFNEGDI
                     IIVEPDGALKDGCFVLAQHAGQWIFRQLVRRGEAWCLHPFNPARTDLADLPLPDLSAV
                     HGLVIQKAVPGRRKLTRHYL"
     gene            complement(1510..2985)
                     /locus_tag="VGA59_12890"
     CDS             complement(1510..2985)
                     /locus_tag="VGA59_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9720617.1"
                     /translation="MAQWVTIWRAAQLLDVPRGVLQQRVRTGEIELTDGLVSTEALLQ
                     LYPLAQIEPSGMLDRVVQIRNDSFARRVRERLLPSQEVLAQRLFHQSQELADVQRHLQ
                     RYHALVVSLRQHIREAQLAAGGDDPRLLALECQLNDGLARALATEPVDRLNVMDDMLK
                     VMSAQVTLRPSGHQYTIEGRATLLQGAIRAGLNLDYGCGNGTCGMCKVRVTSGEVARA
                     IPSDYPLTEAEKARGYVLACACTAASGEVTIETLEAAGPADIPRQQIVAIVRAIRPLA
                     PDTVQLHLQTPRSQRLRFLAGQSVTLGVADGEGAHARHAVSSCPCDDRNLHFFIPRDS
                     EDGLAARLFAGAIKPGDPVTIWGPHGEFALSERAQPLVFAACDTAFAPVKSMIEHALS
                     IDAAPSISLFWLATRPDGHFLANQCRAWSEAIDLFEYTLSSHADAGEGARQIAQAMRA
                     DLFEIDCAFYIAGPQAFADTLVAQLAGAGVPASQIHMETLA"
     gene            complement(2970..3902)
                     /locus_tag="VGA59_12895"
     CDS             complement(2970..3902)
                     /locus_tag="VGA59_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX9720618.1"
                     /translation="MNAPVPALQRPATPFDLDDEVTYRRWRLWKLALRATRIEDLIVD
                     VADPRALTPAEHSALLARIASTNMALYRSPVAAEDKDLPRRLGAQLGLRRLDANWLAD
                     EDGVSRIAVSRSHDGRGGFIPYTDRAIRWHTDGYYHPGERRIHGMILHCVRPARRGGE
                     NALLDHELAYIALRDIDPSYIVALMQPDAMIIPARTGEDGVARAAQTGPVFSVDASTG
                     RLHLRYTARARSIEWKADAATRAAVACLETVLAGAVPWILRVRLRAGMGLVGHNVLHD
                     RSAFEDDASQPRLLYRARFLDRVDLPAEDAWRNG"
     gene            complement(3907..4563)
                     /locus_tag="VGA59_12900"
     CDS             complement(3907..4563)
                     /locus_tag="VGA59_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720619.1"
                     /translation="MTATAKTPPAPRLKSRWFRAGADKSAGQQASAMAFIVWRVARHA
                     LDRMRQAGFDVEVGPPYFAFLREVLVFLVVLADRIAHARLDGDRRTEFTSALVHHLAR
                     ILQDNEDDLLGPAPAGAPSHADSFIDLVNEVSAHYAEFGADPALPVADAGFQPDFAFL
                     RYLGHRLEPTLSEKDRRWVLDQVMAVDAPEAVGMVQRSMRDLFDPGTRRARRSTVSGD
                     "
     gene            complement(4586..4915)
                     /locus_tag="VGA59_12905"
     CDS             complement(4586..4915)
                     /locus_tag="VGA59_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEX9720620.1"
                     /translation="MTYVVDGEELETDEQGYLLQPDWRDEAVRVIAAAENIELTEDHW
                     KVVNYLREQYREHGHTPNFRIMLKDMAALIPGCDSKRLYELCPLGPAKQGAKLAGLPQ
                     PLGKGGY"
     gene            complement(4954..5568)
                     /gene="cas6"
                     /locus_tag="VGA59_12910"
     CDS             complement(4954..5568)
                     /gene="cas6"
                     /locus_tag="VGA59_12910"
                     /inference="COORDINATES: protein motif:HMM:NF021100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated protein
                     Cas6/Cmx6"
                     /protein_id="HEX9720621.1"
                     /translation="MSDERAATMIDVAFALDGNTLPCDHRYALAEAVEGALPWLAGLP
                     GAGVHHLNLVHGTGREAMLSRRTRLTLRVPRERADDACALAGAELNVAGRRLRAGAAQ
                     RRELLRHGTLYAHFVAADGTDELIFLVAVKEELSALGVQCRPICGRRQVGEGGLLMGF
                     SLMLDALSPDHSLRVLEAGLGRHRRLGCGLFVPHRSAAAVGTPS"
     gene            complement(5574..5789)
                     /locus_tag="VGA59_12915"
     CDS             complement(5574..5789)
                     /locus_tag="VGA59_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720622.1"
                     /translation="MTRLNSLLQLRVPVGGQEIELQQLDYEAGGMSLLRTRIREKSRF
                     TVFDIDPLTAEQWGEALVRWARSQPRE"
     gene            5981..7276
                     /gene="dsrA"
                     /locus_tag="VGA59_12920"
     CDS             5981..7276
                     /gene="dsrA"
                     /locus_tag="VGA59_12920"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HEX9720623.1"
                     /translation="MAKKMHDTPMLDELESGPWPSFVTGLKRLAKDKDYMVDLMGQLE
                     TSYRTKKGYWKGGTVGVFGYGGGVIPRFTELKDENDKPVFPDAAEFHTLRVQPPAGMH
                     YNTEVLRKMCDIWERHGSGLIAFHGQSGDIMFQGARTDKVQAAFDELNELGFDLGGAG
                     PAVRTSMSCVGAARCEQSCYDEARAHRQVLNTFVDDIHRPALPYKFKFKFSGCGNDCM
                     NSIQRSDMAVIGTWRDNMRTDEGLARKWFARHGMNELVNDVITRCPTKTIMLKEIKDV
                     AQGDKISSVAVSDTHALEIDNGNCVRCMHCINVMTGALAHGTETGATILIGGKRTLKI
                     GDLMGTVIVPFMPLKTDEDYEALVALGQKVIDFFAENALEHERTGEMIERIGLVNFLE
                     GIGVEIDPNMVAAPRTNPYVRTDGWDDEVARINAKKAAA"
     gene            7308..8378
                     /gene="dsrB"
                     /locus_tag="VGA59_12925"
     CDS             7308..8378
                     /gene="dsrB"
                     /locus_tag="VGA59_12925"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HEX9720624.1"
                     /translation="MATMRQPIESGVPDNKQYMHPTLLKNYGNWRYHDRPRPGVLHHV
                     SHSGDEVWSVRAGTQRQMDVYTVRKLCDIADTYAEGHVRFTIRSNIEFMVSDPNKVAP
                     LVEALTSNGFPVGGTGNSVSMIAHTQGWLHCDIPGTDASGAVKALMDDLYSEFIREDM
                     PNRVHLSTSCCEINCGGQADIAIIVQHTKPPKINHDLVANVCERPAVVARCPVAAIRP
                     ALVNGKPSLEIDEKKCMCCGACYPPCPPMQINDPEYTKLAIWVGGKNSNARGKPTFMK
                     MVASGLPNNPPRWPEVSAMVKKILYTYKEDARPWERLSDWVERIGWPRFFEKCDLPFT
                     KYLIDDWRGSRASLNASTHIRF"
     gene            8395..8754
                     /gene="tusD"
                     /locus_tag="VGA59_12930"
     CDS             8395..8754
                     /gene="tusD"
                     /locus_tag="VGA59_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HEX9720625.1"
                     /translation="MKFGLLVSEGPYTHQASDTAYQFALAALGKGHEIQRVFFYHDGV
                     YNATRLTEPPQDDRHIVNRWSALASEHKVDLVVCVAAALRRGIKEEVLAPGFRISGLG
                     QLVDSGIKADRVVTFGD"
     gene            9263..9619
                     /gene="tusC"
                     /locus_tag="VGA59_12935"
     CDS             9263..9619
                     /gene="tusC"
                     /locus_tag="VGA59_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HEX9720626.1"
                     /translation="MKTFMFVNRKAPYGTIYALESLEVVLIAATFDQDVSLAFLDDGV
                     YELAKGQQTKGIGVKNFSPSYRALEGYDVEKLYVERESLQARGLAEKDLLVPVEVLSS
                     AEMAELMGRQDVVLSF"
     gene            9639..9938
                     /gene="tusB"
                     /locus_tag="VGA59_12940"
     CDS             9639..9938
                     /gene="tusB"
                     /locus_tag="VGA59_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HEX9720627.1"
                     /translation="MLHIVNKSHLQTRSLDSCLRLAQGGHALLLIEDGVYAATRGGAI
                     AAGMAGATQRLKVYALLPDLEARGMAGNVIDGITPVDYGGFVDLVAEHNTCQTWL"
     gene            9976..10308
                     /locus_tag="VGA59_12945"
     CDS             9976..10308
                     /locus_tag="VGA59_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEX9720628.1"
                     /translation="MGIEVNGNTYETDEEGYLINLADWSEDIAGVIAKSENVDMTSSH
                     WEVVTFLRDYYNEYQIAPAVRVLTKAIGKQLGPDKGNSKYLYELFPYGPAKQACKIAG
                     LPKPTGCV"
     gene            10322..11053
                     /locus_tag="VGA59_12950"
     CDS             10322..11053
                     /locus_tag="VGA59_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEX9720629.1"
                     /translation="MSALALGYAALFYAAAAVLAVGLALKIRVYLRAPAPLKIPTTPA
                     PTTTGGVALRLAREVVFFESLFKASKWTWIFGWMFHAALLLVLLRHLRYFQQPVWDII
                     ALVQPFGTYAGFAMVVGLAGLWARRWLVDRVRYISTPSDHLHLALLLAIGLTGLGMRF
                     VRHTDIVAVKTYMLRLMRFDIHPLPADPILLMHLALVALLMFVFPISKLLHAPGLFFS
                     PTRNQADTSREQRHLAAWAAALERK"
     gene            11077..12573
                     /locus_tag="VGA59_12955"
     CDS             11077..12573
                     /locus_tag="VGA59_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX9720630.1"
                     /translation="MATAAFETPKLKPYPVIPLVAVGAMARSKPFVASPAIQAAIGFP
                     GELTVGWQERAVAKMGELLDKYRSLRVYMDACVHCGACSDKCHYFLGTGDPKNMPVAR
                     QDLMRAVYRRYFTFAGKHFPKLVGAVDMTKDVLDQWWTYYNQCSECRRCSVFCPYGID
                     TAEITMAAREIMDSVGLGQKYANEIIGKVHRIGNNLGIPEPALADTLAGLEEDTKEET
                     GIDVRFPLDVEGAEVLLVTPSADFFSEPHVESLIGYAKVFHAAGISWTLSSKASEAGN
                     FGLFIGNYEQMRNISLRVREAAQQLGVRRIVIGECGHAWRVAYSFWNTLIGPFDWLDS
                     RYPVPQHICEFTHDLIGRGALKFDKEKNDDRIITFHDSCNVARASRMGTMPGGQFIIP
                     REIIKAVANRFHDMAPETIHESTFCCGGGGGLLTDDLLELRVKGALPRMEALKNVVDE
                     HGVNFMATICAICKAQFSKVLPYYGFDMSMVGGVHQLVGKAIRLGEPH"
     gene            12597..>13831
                     /locus_tag="VGA59_12960"
     CDS             12597..>13831
                     /locus_tag="VGA59_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9720631.1"
                     /translation="MSAPTESVQVKPLTFRRYKDGDSRPRAWQEEIFDADHSHKCPTY
                     VQSTPPCQGSCPSGEDIRGYLNIVRGVEKPPAGMPWQEYAWRRLTEANPLPSVMGRVC
                     PAPCESGCNRNELEDYVGINSVEHFLGEWAIEHQLAFPKPAVRTGKKVAVIGGGPAGL
                     SAAYQLARKGHAVTIFDERAALGGMMRYGIPGFRTPRATLDAEIQRILDLGVEARMNC
                     RIGTDVTLEQVRADYDAMFFGLGAQAGRPLAVEGADAPNCVTATAFLKAFNDGRMRHV
                     GKRVVVVGGGDTSIDVATVARRLGHIESQHESDRPELVIAGYAAHDVASVSARQGAEV
                     TLTSLFPVDKMQANRHEIEQALSEGIAIRGGLVPVAVVRDAGGRAIALRVARCEARIV
                     GGRLDIKQIEGTEEDIEADL"
BASE COUNT         2363 a         4679 c         4609 g         2180 t
ORIGIN      
        1 ctggccgact tgcgcaatca gctggccggc gcggtgctga tgggtttcgg cggcgtcacg
       61 gccctgggct gcacgatcgg ccagggcctg gcgggtgtct caacgctggc gatcggctcc
      121 ctgatcgccg tcgccggcat cgcggcggga tgcataggca cgctcaagat cattctttgg
      181 cagtcccagc gcgaaggctg aagcagcatg gattgcgggt caagcccgca atgacaaata
      241 ggttgtcatg ccgggctcaa tccggcatcc atgaattcgg acttcctgga ttgcggagcc
      301 gggccggagc gtgatgcctc ggcacgtgca tagcgtaggc ccggcattgg gctcgctgca
      361 ctggacagct gcgcgccccg agcgttccgc gagtccagtc cgcaatgaca aataggttgt
      421 catgccgggc tcgacccggc atccatgaat tcggtattcg caatgacagg tctcgtattg
      481 ctagcccatc atcgcccggt ccgtgcgcgc cgccgcggct gccatgcggc gatcgccgcc
      541 acgatcgccg tccacggcag ctcggtgagg ctgccgtgcc tctcgtgtgc ggtgcggacc
      601 atgcggtgca catcggaatc ggccaccgcg agttcccggc gcagcgcgct caagccgccg
      661 cactgcaatc gaaggctctg cgcgtggatc agcggatgcc ccggcgccgg caggcgcagc
      721 gcaaaccggg cccgttcgtg caataaggcc gccagtgtgc cgcagttgac gcgtgccacc
      781 ggcgaggtgc attccagcag gtcgcgctcg gcgatggcgc gccgctgcga cagttcgcaa
      841 gtcgctgcgc gcgccagcag cgccttggcg aaaacgcacg cgtgatcggt gtcgcagcac
      901 gcggaccccg ggaccaggga atcgtccatc tcctcagtcg tcgccgtccg gcggtgcctc
      961 gcgcacctgg agcatcaagt cctcggccgc aagcgggtta tcggcgaata tcaccttgat
     1021 cctggcagct gggccctcgg cacgcagcgc tttggcttgc atggaggctt ccttgaaact
     1081 ggcgaactcc gccagtttct cgagctgggc catcggcttg atggcgtaaa cgtaatacgg
     1141 catgctacag gtagtgtctt gtgagtttgc ggcgtcccgg aacggccttc tggatcacca
     1201 ggccgtgcac ggccgaaagg tccggcagcg gcaggtcggc cagatcggta cgggcggggt
     1261 tgaacggatg caggcaccag gcctcgccgc ggcgcacgag ctggcggaag atccactgcc
     1321 ccgcatgctg ggccagcacg aagcagccgt ccttcaaggc gccgtcgggc tcgacgatga
     1381 tgatgtcccc ttcattgaat tccggcgcca tatcgtcgcc gagtacgcgc agcgcgaacg
     1441 actcgccggc gctgcacccg ccgtcgtcat gagggtcggc tggggcgggc ggtgcgccgc
     1501 aagtgcaact catgcgagcg tctccatgtg aatctgcgat gcgggaacgc cggcgcccgc
     1561 cagttgcgcg accagcgtgt cggcgaaggc ctgcggccct gcgatataga acgcgcagtc
     1621 gatttcgaac aggtccgcgc gcatcgcttg cgcgatctgg cgcgcgcctt cgccggcgtc
     1681 ggcgtgggaa gagagcgtgt attcgaacag gtcgatcgct tccgaccagg cccggcactg
     1741 gttggccagg aagtgcccgt cgggccgggt cgccagccag aacagcgaga tcgaaggcgc
     1801 ggcatcgatc gacagggcgt gctcgatcat gcttttcacc ggcgcgaaag cggtgtcgca
     1861 ggcggcaaag accagtggct gggcgcgctc ggacagcgcg aactccccgt gcggccccca
     1921 gatcgtcact ggatcgcccg gcttgatcgc gcccgcgaac agccgggcgg ccagcccgtc
     1981 ttcggagtcg cgcgggatga agaagtgcag gttgcggtcg tcgcagggac agctggagac
     2041 cgcgtgccgg gcgtgggcgc cttcgccgtc ggccacgcca agcgtcaccg actggccggc
     2101 caggaagcgc aggcgctggc tgcgcggcgt ctgcaggtgc agctggaccg tgtccggggc
     2161 cagcggccgg atcgcgcgca cgatggcgac gatctgctgg cgcgggatgt cggctggccc
     2221 ggccgcttcc agggtctcga tcgtcacttc gccgctggcc gccgtgcacg cgcaagccag
     2281 gacgtagcct cgcgcctttt cggcctccgt gagcggatag tcgctgggta tcgcccgcgc
     2341 cacctctccg gaggtgacgc gcaccttgca cattccgcag gtgccgttgc cgcagccgta
     2401 gtcgaggttc agcccggcgc ggatggcccc ctgcagcagc gtggcgcggc cctcgatggt
     2461 gtattgatgg ccgctcgggc gcagggtcac ctgcgccgac atcaccttga gcatgtcgtc
     2521 catcacattc agcctgtcga ccggttcggt ggccagtgcc cgcgccagcc cgtcgttcag
     2581 ctggcactcc aacgcgagca ggcgggggtc gtcgccaccc gccgccaact gcgcttcgcg
     2641 gatgtgctgg cgcagcgaca cgaccaacgc gtggtaacgc tgcaggtggc gctgcacgtc
     2701 ggcgagttcc tggctctggt gaaacagccg ctgcgccagc acttcctggc tgggcagcag
     2761 gcgctcccgc acgcgccggg cgaaagagtc gtttcgaatc tggaccacac gatcgagcat
     2821 gccggagggc tcgatctgtg caagcggata aagctgaagc agcgcctcgg tggaaaccag
     2881 cccgtcggtc agttcgatct ctccggtccg cacgcgctgc tgcagcacgc cgcgcggcac
     2941 gtccaacagt tgcgcggccc gccagatggt cacccattgc gccatgcgtc ctccgccggc
     3001 aggtcgaccc gatccaggaa gcgcgcgcga tagagcagcc gcggctgcga ggcatcgtcc
     3061 tcgaaggcgc tgcgatcgtg cagcacgttg tggccgacca gccccatgcc cgcccgcagc
     3121 cgcacgcgca ggatccaggg cacggcgccg gccagcacgg tttccaggca ggctacggca
     3181 gcccgggtgg cggcgtcggc cttccattcg atcgaccggg cgcgggccgt gtagcgcagg
     3241 tgcaggcggc cggtggaggc gtcgacgctg aacaccggcc cggtctgcgc cgcgcgcgcg
     3301 acgccgtcct cgccggtgcg cgcggggatg atcatcgcgt ccggctgcat cagcgcgact
     3361 atgtagctag ggtcgatatc gcgcagcgcg atgtaagcca gctcgtggtc gagcagagcg
     3421 ttctccccgc cgcggcgggc cgggcgcacg caatgcagga tcatgccgtg aatgcggcgc
     3481 tcgcccgggt ggtaatagcc gtcggtgtgc cagcggatgg cccggtcggt gtaggggatg
     3541 aagccgccgc ggccgtcatg cgagcgcgac accgcgatgc gcgacacgcc gtcctcgtcg
     3601 gccagccagt tcgcgtcgag ccggcgcagc cccagctgtg cgcccagccg gcgcggcagg
     3661 tccttgtcct ccgctgcgac gggggagcgg tacaaggcca tgttcgtgct ggcgatgcgg
     3721 gcaagaagcg ccgagtgctc ggccggtgtc aatgcgcgcg gatcggccac gtcgacgatc
     3781 aggtcttcga tccgggtggc gcgcagagcg agcttccaga ggcgccagcg ccggtacgtg
     3841 acctcgtcat cgaggtcgaa cggggtcgct ggacgctgca gggccggaac aggggcgttc
     3901 atcgcgtcaa tccccgctca ccgtggatcg gcgggcgcgc cgcgtcccgg ggtcgaacag
     3961 atcgcgcatc gaacgctgca ccatgcccac cgcttcgggc gcgtcgacgg ccatcacctg
     4021 gtccagcacc cagcgccggt ccttctcgga cagggtcggc tccagcctgt gccccaggta
     4081 gcgcaggaag gcgaagtccg gttgaaagcc cgcgtccgcg accggcagcg ccgggtcggc
     4141 gccgaattcc gcataatgcg cgctgacttc gttgaccagg tcgatgaaac tgtctgcatg
     4201 cgagggcgct cccgcgggcg cgggtcccag caagtcgtcc tcattgtcct gcaggatgcg
     4261 ggcgagatgg tgtacgaggg cggaggtgaa ctcggtgcgc cgatcgccat cgagccgcgc
     4321 atgggcaatc cggtcggcca gcacgaccag gaacaccagg acttcgcgca ggaacgcgaa
     4381 gtagggcggc cccacctcga cgtcgaaacc cgcctgtcgc atccggtcca gcgcatggcg
     4441 cgccacccgc cagacgatga acgccatcgc gctggcctgc tgcccggcgc tcttgtccgc
     4501 gccggccctg aaccaacggc tcttcaggcg cggcgccggc ggggttttcg cggtcgcggt
     4561 cacgggagtc gcctcgcgct tgcggtcaat agccgccctt gcccagcggc tggggcaggc
     4621 cggcgagctt ggcgccctgc ttggcggggc cgagcggaca cagctcgtac aaccgcttgc
     4681 tgtcgcaacc cggaatgagg gcagccatgt ccttgagcat gatgcggaag ttcggcgtgt
     4741 ggccgtgctc gcggtactgc tcgcgcaggt aattgaccac cttccagtgg tcctcggtga
     4801 gttcgatgtt ctcggccgcc gcgatcaccc tcacggcttc gtcgcgccag tcgggctgca
     4861 gcagatagcc ctgttcgtcg gtttcgagtt cctcgccgtc aacaacgtaa gtcatgttcc
     4921 cgatgttcct ttcacgtgaa atggcgcggc acgtcaggac ggcgtcccga cggccgcggc
     4981 ggatctgtgg ggcacgaaca gcccgcagcc caaccggcga tgccggccta gaccggcctc
     5041 cagcacgcgc agcgagtggt cgggcgatag agcgtcgagc atcaggctga agcccatcag
     5101 caagccacct tcccccacct gccgccgccc gcagatcggc cggcattgca caccgagcgc
     5161 tgagagttct tctttcacgg cgaccaggaa gatcagttcg tccgtgccgt cggccgccac
     5221 gaagtgagcg tagagcgtgc cgtgccgcag cagttcccgg cgctgcgcag cgcccgcccg
     5281 cagccggcgg ccggcaacgt tcagctcggc gccggccagg gcgcaggcgt cgtccgcgcg
     5341 ttcgcgaggc acgcgcagcg tcagccgcgt gcgcctggac agcatggctt cgcgacccgt
     5401 gccatggacc agattgaggt ggtgcacgcc ggcgccgggc aggccggcca gccaaggcag
     5461 cgcaccctcc acggcctcgg ccaacgcgta ccggtggtcg cacggcagcg tgttgccgtc
     5521 gagcgcgaag gcgacatcga tcatggtggc ggcgcgctcg tcggacaccg gcttcactcc
     5581 cgcggctgcg agcgcgccca acgcaccagg gcctcgcccc actgttcggc cgtgagcggg
     5641 tcgatgtcga acaccgtgaa gcggctcttc tcgcggatgc gggtgcgcag caggctcatg
     5701 ccgccggctt cgtaatcgag ctgctgcaat tcgatctcct gcccgcccac gggcacgcgc
     5761 agttggagca gggaattcag gcgcgtcatc ggaggcattg agcgcgatgc cgcgtgcgcc
     5821 cgtccatacc cggtacgggg gaccggcgca taccccttcc gggtagtgcc gccatcctca
     5881 acgagagtga tagccgatcg cgcgccgccc ggcgaggatt cactccattc aaccgaggtc
     5941 gcgcgctgtc gccggcccca cgagccagga gacaaccgaa atggccaaga agatgcacga
     6001 tacgccgatg ctcgacgagc tcgagagcgg cccgtggccg agcttcgtga ccggcctgaa
     6061 gcggctggcc aaggacaagg actacatggt tgacctgatg ggtcagctcg agacctcgta
     6121 ccgcacgaag aagggctact ggaagggcgg caccgtcggc gtgttcggtt atggcggcgg
     6181 cgtgatcccc cgcttcaccg agctcaagga cgagaacgac aagcccgtct tcccggacgc
     6241 cgccgagttc cacacgctgc gcgtgcagcc gccggccggg atgcactaca acaccgaggt
     6301 gctgcgcaag atgtgcgaca tctgggagcg ccacgggtcc ggcctgatcg cctttcacgg
     6361 ccagtcgggc gacatcatgt tccagggcgc caggacggac aaggtgcagg ccgcgttcga
     6421 cgagctgaac gaactcggct tcgacctcgg aggcgccggc cccgcggtgc gcacctcgat
     6481 gtcctgcgtc ggcgccgcgc gttgcgagca gtcgtgctac gacgaggcgc gggcgcaccg
     6541 ccaggtgctc aatacgttcg tggacgacat ccaccgcccg gccctgccct acaagttcaa
     6601 gttcaagttt tcgggctgcg gcaacgactg catgaactcg atccagcgct ccgacatggc
     6661 cgtcatcggc acctggcgcg acaacatgcg caccgacgag ggactggccc gcaagtggtt
     6721 cgccaggcac ggcatgaacg agctggtcaa cgacgtgatc acgcgctgcc cgaccaagac
     6781 catcatgctc aaggagatca aggatgtcgc ccagggcgac aagatttcca gcgtcgccgt
     6841 gagcgacacc catgcgctgg agatcgacaa cggcaactgc gtgcgctgca tgcactgcat
     6901 caacgtcatg acaggggcgc tcgcgcacgg caccgaaacc ggcgcgacga tcctgatcgg
     6961 cggcaagcgc accctgaaga tcggcgacct gatgggcacc gtgatcgtgc cgttcatgcc
     7021 gctcaagacc gacgaagact acgaggcgct ggtcgccctc ggccagaagg tcatcgactt
     7081 cttcgccgag aacgcgctcg agcacgagcg caccggcgag atgatcgagc gcatcggcct
     7141 ggtcaacttc ctcgaaggca tcggtgtcga gatcgacccg aacatggtcg cggcgccgcg
     7201 caccaacccc tacgtgcgca ccgacggctg ggacgacgag gtcgcgcgga tcaatgcgaa
     7261 gaaggcggcc gcctgaaccg gcgccccacc aacacggaag gcgagccatg gccacgatgc
     7321 gacaaccgat cgagagcggt gtaccggaca acaagcagta catgcacccg accctgctga
     7381 agaactacgg caactggcgc tatcacgacc ggccaaggcc cggcgtgctg caccatgtgt
     7441 cgcacagcgg cgacgaagtc tggtcggtgc gcgccggcac gcagcgccag atggacgtct
     7501 acaccgtccg caagctctgc gacatcgccg acacctacgc cgaagggcat gtgcgcttca
     7561 cgatccgcag caacatcgag ttcatggtgt ccgacccgaa caaggtcgcc ccgctggtcg
     7621 aggccctgac cagcaacggc ttcccggtcg gcggcaccgg caactcggtg tccatgatcg
     7681 cccataccca ggggtggctg cactgcgaca tcccgggcac cgacgcctcg ggcgcggtga
     7741 aggcgctgat ggatgacctg tacagcgagt tcatccgcga ggacatgccc aaccgcgtgc
     7801 acctgtcgac ctcgtgctgc gagatcaact gcggcggaca ggccgacatc gcgatcatcg
     7861 tgcagcacac caagccgccc aagatcaacc atgacctggt ggccaacgtc tgcgaacgcc
     7921 cggccgtggt ggcccgctgt ccggtcgcgg cgatccggcc ggcgctggtc aacggcaagc
     7981 cgtcgctcga aatcgacgag aagaaatgca tgtgctgcgg cgcctgctac ccgccctgcc
     8041 cgccgatgca gatcaacgac cccgagtaca ccaagctcgc gatctgggtg ggcggcaaga
     8101 actccaacgc ccgcggcaaa ccgaccttca tgaagatggt ggcctcgggc ctgccgaaca
     8161 acccgccgcg ctggccggaa gtctcggcca tggtcaagaa gatcctctac acgtacaagg
     8221 aagacgcgag gccctgggag cggctgtccg actgggtcga gcgcatcggc tggccgcgct
     8281 tcttcgagaa gtgcgacctg cccttcacca agtacctgat cgacgactgg cgcggttcgc
     8341 gtgcgagcct gaacgcctcc actcacatcc gtttctgaag gtcggatact cacgatgaaa
     8401 ttcggcttgc tcgtcagcga agggccctac acccaccagg ccagcgacac cgcctaccag
     8461 ttcgcgctgg cggcgctggg caagggccac gagatccagc gcgtcttctt ctatcacgac
     8521 ggggtctaca acgcgacccg gttgaccgag ccgccgcagg acgaccgcca catcgtcaat
     8581 cgctggtcgg cgctggcctc cgaacacaag gtcgacctcg tggtctgcgt ggccgccgcg
     8641 ttgcggcgcg gcatcaagga ggaagtgctg gcgccgggct ttcgcatctc gggcctcggc
     8701 cagctcgtgg acagcggcat caaggccgac cgtgtcgtga ccttcggaga ctagcaatga
     8761 gccgtaacgg accccatgct gtcatgccga gccacttcga ctggctcagg acaggctcga
     8821 cccggcatcc acgcggcgat cgcacctgcg ttgcatggat tgcggggccg ggccggagcg
     8881 tgatgcctcg gaacgtgcat agcgtaggcc cggcattggg ctcgctgcac tggacaactt
     8941 cgcgccccgg gcgttccgcg agcccagccc gcaatgacaa acctgggtgt catgccgggc
     9001 ttggcctcag gtgtcatgcc gggcttggcc tcaggtgtca tgccgggctt gacccggcat
     9061 ccatgacttc ctggattgcg gggccgggcc ggagcgtgat gcctcggcac gtgcatagcg
     9121 taggcccggc attgggctcg cttcactgga cagctgcgcg ccccgggcgt tccgcgagcc
     9181 cagcccgcaa tgacatgcct tccaccaggg cctggattgc aagtgaagcc cgcaatgaca
     9241 tgcctctcac caggcgtcag gcatgaagac gttcatgttc gtcaaccgca aggcgcccta
     9301 cggcacgatc tacgcgctcg agagcctgga ggtggtgctg atcgccgcga ccttcgacca
     9361 ggacgtgagc ctggctttcc tcgacgacgg cgtgtacgaa ctggccaaag gccagcagac
     9421 caagggcatc ggcgtcaaga acttctcgcc aagctatcgc gcgctcgaag gctacgacgt
     9481 cgagaaactc tatgtggagc gtgagtcgct gcaggcgcgc ggcctggccg aaaaggacct
     9541 gctggtgccg gtcgaagtgc tctccagcgc cgaaatggcc gagctgatgg gccggcagga
     9601 cgtggtcctg agcttctaga ttgccgcgaa ggaaacccat gctgcacatc gtcaacaagt
     9661 cgcacctgca gacacgctcg ctggacagct gcctgcgcct ggcgcagggc ggccatgcgc
     9721 tgctgctgat cgaggacggc gtgtatgccg ccacccgcgg cggtgcgatt gccgccggca
     9781 tggccggggc cacccagcgc ctgaaggtct atgccctgct gcccgacctg gaggcgcgcg
     9841 gcatggccgg caacgtgatc gatggcatca cgcccgtcga ttacggcggc ttcgtcgatc
     9901 tcgtcgccga gcacaacacc tgccagacct ggctctgaag accggtctgc catcccaacg
     9961 cacaaggagc actacatggg catcgaagtc aacggcaaca cctacgagac cgacgaggaa
    10021 gggtacctga tcaacctggc cgactggagc gaggacatcg ccggcgtgat cgccaagtcg
    10081 gaaaacgtcg acatgacgtc aagccactgg gaggtcgtca ctttcctgcg cgactactac
    10141 aacgagtacc agatcgcgcc cgcggtccgg gtgctgacca aggcgatcgg caagcagctg
    10201 gggcccgaca agggcaacag caagtacctg tacgagctgt tcccctacgg gcccgcgaaa
    10261 caggcctgca agattgccgg gctgcccaag cccaccggct gcgtctagcc ggttcacaac
    10321 gatgtcggcg ctcgccttgg gctatgcggc tctcttctac gccgcagcgg cggtgctggc
    10381 cgtcgggctg gcgctgaaga tacgtgtcta cctgcgcgct ccggcaccgc tgaagatccc
    10441 gacgacgccg gcgccgacca ccaccggcgg ggtcgccttg cgcctggccc gcgaggtggt
    10501 tttcttcgag agcctgttca aggccagcaa atggacctgg atcttcggct ggatgttcca
    10561 cgccgcgctg ctgctcgtac tgctgcgcca ccttcgctac ttccagcagc cggtgtggga
    10621 catcatcgct ttggtgcagc cgttcgggac gtatgccggc tttgcgatgg tcgtgggcct
    10681 ggccggcctg tgggcgcgcc gctggctggt cgatcgcgtg cgctacatct ccacgccgtc
    10741 cgaccacctg catctggcgc tgctgctcgc gatcggcctc accggcctgg gcatgcgttt
    10801 cgtgcgccac accgacatcg tcgcggtgaa gacctacatg ctgcggttga tgcgattcga
    10861 catccatccc ctgccggcgg acccgatttt gctgatgcat ctggcgctgg tggcgctgct
    10921 gatgttcgtg ttccctatca gcaagctgct gcatgcgccg gggctcttct tcagcccgac
    10981 tcgcaaccag gccgatacgt cgcgtgagca gcgccacttg gccgcctggg ccgccgcgct
    11041 ggaacgcaag tagcagcccg caaaggacca gccgttgtgg ccaccgccgc cttcgagaca
    11101 cccaagctca agccctaccc ggtgatcccg ctggtggccg tgggcgcgat ggcgcgcagc
    11161 aagcccttcg tcgcctcgcc ggcgatccag gccgcgatcg gctttcccgg cgagctcacc
    11221 gtgggctggc aagagcgtgc cgtcgccaag atgggcgagc tgctggataa gtaccgttca
    11281 ctgcgcgtct acatggatgc ctgcgtgcat tgcggcgcct gttccgacaa gtgccactac
    11341 ttcctgggca cgggcgaccc gaagaatatg ccggtggcgc gccaggacct gatgcgcgcg
    11401 gtgtaccgcc gctacttcac cttcgcaggc aagcacttcc ccaagctcgt gggcgcggtc
    11461 gacatgacaa aagacgtgct cgaccagtgg tggacctact acaaccagtg ctccgaatgc
    11521 cgccgctgct cggtgttctg cccgtacggc atcgacaccg ccgagatcac catggcggcg
    11581 cgcgagatca tggactcggt gggactgggc cagaaatacg ccaacgagat catcggcaag
    11641 gtgcaccgca tcggcaacaa cctgggcatt cccgagccgg cgctcgccga cacgctggcc
    11701 ggtctggaag aggacacgaa ggaggagacc ggcatcgacg tgcgcttccc gctcgacgtg
    11761 gagggcgccg aagtgctgct ggtcacgccc tcggccgact tcttctccga gccgcatgtc
    11821 gaaagcctga tcggctacgc caaggtgttc cacgccgccg gcatcagctg gacgctctcc
    11881 tccaaggcct cggaagcggg caacttcggc ctgttcatcg gcaactacga gcagatgcgc
    11941 aacatctcgc tgcgcgtgcg cgaggcggca cagcagctag gcgtgaggcg catcgtgatt
    12001 ggcgagtgcg gccatgcctg gcgcgtggcc tacagcttct ggaacaccct gatcggcccc
    12061 ttcgactggc tcgattcccg ctacccggtg ccgcagcaca tctgcgaatt cacgcacgac
    12121 ctcatcggcc gtggcgccct caagttcgac aaggaaaaga acgacgaccg catcatcacc
    12181 ttccacgatt catgcaatgt cgcgcgtgcc tcgcgcatgg gcaccatgcc gggcgggcag
    12241 ttcatcatcc cgcgcgagat catcaaggcg gtggccaacc ggttccacga catggcgccg
    12301 gaaaccatcc acgagagcac cttctgctgc ggtggcggcg gcggcctgct cacagacgac
    12361 ctgctggagc tgcgcgtcaa gggcgcgctg ccccgcatgg aagcgctcaa gaacgtcgtg
    12421 gacgaacacg gcgtcaattt catggcgacg atctgcgcga tctgcaaggc gcagttcagc
    12481 aaggtgctgc cttactacgg cttcgacatg agcatggtcg ggggcgtgca ccagctcgtc
    12541 ggcaaggcga tccggctcgg cgagccgcat tgatcccaac tccccaggaa aaccccatgt
    12601 ccgctccgac cgaatctgtc caggtcaagc cgctgacctt ccggcgttac aaggacggcg
    12661 acagccggcc gcgcgcgtgg caggaggaga tcttcgacgc cgaccattcg cacaagtgcc
    12721 ccacctatgt gcagagcacg ccgccctgcc agggcagctg cccgtcgggc gaggacattc
    12781 gcggctatct caacatcgtg cgcggcgtcg agaagccgcc cgctggcatg ccctggcagg
    12841 aatacgcctg gcgccgcctg accgaggcca accccctgcc gtcggtgatg ggacgggtct
    12901 gtccggcgcc ctgcgagtcg ggctgcaatc gcaacgagct cgaggactac gtcggcatca
    12961 actcggtgga gcattttctc ggcgagtggg ccatcgagca ccaactggcg ttccccaagc
    13021 ccgctgtgcg caccggcaag aaggtggcgg tgatcggcgg cggcccggcc gggctgtcgg
    13081 cggcgtacca gctcgcgcgc aaaggccatg ccgtcacgat cttcgacgag cgcgccgcgc
    13141 tcggcgggat gatgcgctac ggcatccctg gattccgcac cccgcgtgcg acgctcgacg
    13201 cggagatcca gcgcatcctc gacctgggtg tcgaggccag gatgaactgc cgcatcggca
    13261 ccgacgtgac tctcgagcag gtgcgcgccg actacgacgc catgttcttc ggcctgggtg
    13321 cgcaggccgg ccgaccactg gcggtggaag gagccgatgc gcccaactgc gtcaccgcga
    13381 ccgccttcct gaaagccttc aacgacgggc gcatgcgcca cgtgggcaag cgggtggtgg
    13441 tggtgggcgg cggcgacacg tcgatcgacg tggccaccgt ggcgcggcgc ctgggccata
    13501 tcgagagcca gcacgagagc gaccgcccgg agttggtgat cgccggctat gcggcgcacg
    13561 atgtcgcctc ggtatccgcc cgccagggtg cggaagtgac gctgacctcc ctgttcccgg
    13621 tcgacaagat gcaggcgaac aggcacgaaa tcgagcaggc cctgagcgag ggcatcgcca
    13681 tccggggcgg cctggtgccg gtggcggtgg tgcgagatgc gggcggccgc gccatcgcac
    13741 tgcgggtggc gcgctgcgaa gccaggatcg ttggcggccg gctggacatc aagcagatcg
    13801 aaggcacgga agaagacatc gaggccgacc t
//
LOCUS       DASWIY010000363         8913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_985927, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000363 DASWIY010000000
VERSION     DASWIY010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985927"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..2875)
                     /gene="polA"
                     /locus_tag="VGA59_12965"
     CDS             complement(38..2875)
                     /gene="polA"
                     /locus_tag="VGA59_12965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX9720632.1"
                     /translation="MSEPTNEQNANSQPPKTLLLVDGSSYLYRAYHAMPDLRAVPGDP
                     ASPATGAIRGMINMLQKLRKDVRADYVACVFDAPGPTFRDSLYPAYKATRSPMPPDLR
                     LQIDPIHEVVRLLGWKVLNIPGVEADDVIGTLSCMATERGIETVISSGDKDLSQLVNE
                     HVTVIDTMNDRKRDVAGVEAEFGVPPSLMVDYQTLVGDAVDNVPGVEKVGPKTAVKLL
                     QEYGSLDALIARVHEVKGAVGENLRKALDWLPKGRELLTIKKDCELKDHIPGLPSLED
                     IAVTGGQDTEALKAFYDRYGFKGLVKQLETHEVPPELLEEHRSKRKVGPGAGPAGLFE
                     EPDLSGLSQAANLQYDTIFTWERFDEWLARLEAAELAAIDTETSSLDEMQAQIIGLSF
                     SVKPGEAAYIPLAHSYGDAPDQLPLDQVLARLKPWLENPDKLKLGQHVKYDRHVFANH
                     GIEVGGYAHDTMLQSYVLEVHKPHGLASLAERHLGRSGIDYETIAGKGAHQIGFAQVS
                     VDKAAEYSCEDSDQTLDVHLVLWPRLQADSKLRFIYDLEMRSSETLYRIERNGVLIDG
                     PILARQSAELGQRIMQLEREAHELAGQPFNLGSPKQIGDILFTKLGLPVLKKTPSGVA
                     STDEEVLEKLAEDYPLPAKLLEHRGLSKLKGTYTDKLAQLAHPRTGRVHTHYAQAVAV
                     TGRLSSNEPNLQNIPIRTPEGRRVREAFIAPPGNFIASADYSQIELRIMAHLSGDEAL
                     LRAFKEGMDVHRATASEVFGVAPEQVTSEQRRYAKVINFGLIYGMSSFGLARNLGIDN
                     TAAKNYIQRYFERYPGVKRYMDETRQSAKSKGYVETVFGRRLYLPEINSPNGPRRGGA
                     ERAAINAPMQGTAADLIKLSMVKVQDVLDAEQRRTKMIMQVHDELVFEVPEDEVEWVR
                     IEIPRLMAGVAELRVPLLAEIGIGENWDKAH"
     gene            3185..3508
                     /locus_tag="VGA59_12970"
     CDS             3185..3508
                     /locus_tag="VGA59_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720633.1"
                     /translation="MHPRLMLLAPLLACAAVAAQTVPPPQAPAPAEGRKNQRIERIHH
                     EDAGARIDELRYGGETQSIVVQPKADVPEYEIQPTDLARSRPADHRDGMSSATGQRVW
                     NLFRF"
     gene            3530..4504
                     /locus_tag="VGA59_12975"
     CDS             3530..4504
                     /locus_tag="VGA59_12975"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX9720634.1"
                     /translation="MAVFTEVSESEAGELAHTLQLGRLRELRGIPGGIENTNYFLTTD
                     QGDYVLTLFERLTFEQLPFYLHLMKHLAQRGIPVPEPQAIPGSASAGDPDGEILLEVR
                     GKPAAVVDKLRGKSELAPTAAHCAAVGAMVARMHLAGRDYPRRQPNLRGLAWWNETVP
                     VVLPYVDEAQAALLRSELAFQNHVAAGSAYSALPRGVIHADLFRDNALFEQGRLTGFF
                     DFYFAAVDSWLFDLAVCLNDWCIDLATGAHDADRAAAFLRAYAGVRPLAAAEWQLLPA
                     MLRAGALRFWISRLWDFHLPRDAAMLQPRDPVHFERVLRQRAAHPVGL"
     gene            4509..5270
                     /locus_tag="VGA59_12980"
     CDS             4509..5270
                     /locus_tag="VGA59_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEX9720635.1"
                     /translation="MKLNIVPARTGVQWVKLGMKTFAKQPLALAGLFFMYMAAVIAVA
                     QVPVVGVVIGGMLVPAATLGLMAATAEATKGRFPMPSVLISAFRAGRQQVRAMLLLGV
                     IYTVGSKLMTELATLVMGTPAADSGEVDGATLLALVLHTPLFLMFWHAPALVHWHGVT
                     PAKSLFFSMVACLKNFGAFLVYGLTWLVVFLAVGMVFGLMGGLLGGPEMARAVMMPTA
                     LLMAAMFSTSIYFTFLDSFVATPEEPELQPPGEPL"
     gene            5267..5983
                     /locus_tag="VGA59_12985"
     CDS             5267..5983
                     /locus_tag="VGA59_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron dicitrate transport regulator FecR"
                     /protein_id="HEX9720636.1"
                     /translation="MTQHILQGRSEDEILWFRRRGFLQAAAAFSAMGGLAAAQAQQRS
                     NIVELRGDVLLNGERLHAQRTIQTGDSLETGPGSHLVFVIGNSAFQVRQNSRLVVERG
                     ATLNTVSVLRMITGAVASVWGRGTSRQIVTPTLTAGIRGTGVYTEVFPQQGYRSYFCN
                     CYGTVDVDAGGDKTVSRAEYHQSFWGEVEPKNGRYLTPARMINHTDEELELLARLVNQ
                     RTAWQIAGRKGAADGSGYMK"
     gene            complement(6226..7074)
                     /locus_tag="VGA59_12990"
     CDS             complement(6226..7074)
                     /locus_tag="VGA59_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9720637.1"
                     /translation="MMQNTMAIPSSPHTVARPPRRPINAKADRHFVTALARGLDVLSA
                     FRSRDRMLGNQELSRRCGLPKSTISRLTYTLTKHGYLEHADDGSGNTGYRLGSAVLAL
                     GSTMLARMDMRQIARPLMQELADHSHAMVSLGMRDRLSMIYVENCRSEAALTLSLDVG
                     SRIPLATTAMGRAYLAQCGEADRDNLLKRIRERDEAAWPRVREGIARALAEYRELGCS
                     TSFGEWQKDVNAIAIAFRPPGGRSIMAINCGGPSFNLSREFLLDKVRPPLVALAKRLQ
                     GSGGAD"
     gene            complement(7437..>8913)
                     /locus_tag="VGA59_12995"
     CDS             complement(7437..>8913)
                     /locus_tag="VGA59_12995"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9720638.1"
                     /translation="LVRGDGPRGGYERHEIAVLYRSNAQSRVIETALFNAAMPYRVYG
                     GLRFFERAEIKHALAYLRLLENPHDDTSFLRIVNFPPRGIGARSIEQLQDAARAAGCS
                     LHDAVSATTGKAGTNLTAFVAKIDALREQTLNLTLREIIELVLQASGLIEHYKSEREG
                     ADRIENLEELVNAAESFVTQEGFGRDAVALPVDELSQSPVSQGLDPNAPSPQPSPANG
                     RGGRELLPLPPGERGRGGRELLPLPPGEGGGEGAAPDGARPGLRSGVPPDAETGETLS
                     PLAAFLTHAALESGDNQAQAGQDAIQLMTVHSAKGLEFDCVFISGLEEGLFPHENSMS
                     DHEGLEEERRLMYVAITRARKRLYLSHAQTRLLHGQTRYNVKSRFFDELPEGAMKWLT
                     PKNQKFVGSAFGFGGGYATSRGATAGSRGKDVFASPPVPARKSPSHGLRADMKVFHTK
                     FGEGTVMSIEGQGDDARAQVNFPRHGVKWLALSVAKLTPVT"
BASE COUNT         1379 a         2947 c         2950 g         1637 t
ORIGIN      
        1 gcctccgcgg ggatgacgaa agagcccccg cccttagtta atgcgccttg tcccagttct
       61 cgccgatgcc gatctcggcg agcagcggca cgcgtaactc ggccacaccg gccatgaggc
      121 gcggaatttc aatgcggacc cactcgacct cgtcctcggg cacttcgaac accagttcgt
      181 cgtgcacctg catgatcatt ttcgtgcggc gctgctcggc gtcgagcacg tcctgcacct
      241 tcaccatgga gagcttgatc aggtcggccg ccgtgccctg catcggcgcg ttgatggccg
      301 cgcgctcggc gccgccgcgg cgcgggccat tgggtgaatt gatctcgggc aggtacaggc
      361 gccggccgaa cacggtctcg acatagcctt tgctcttggc cgattgccgc gtctcgtcca
      421 tgtagcgctt gacgccggga tagcgctcga agtaacgctg gatgtagttc ttggccgcgg
      481 tgttgtcgat gcccaggttg cgcgccaggc cgaagctgct catgccgtag atcaagccga
      541 agttgatgac cttggcatag cggcgctgct cgctggtcac ctgctcgggc gcgacgccaa
      601 acacctccga cgcagtcgcg cggtgcacgt ccatgccttc cttgaaagcg cgcagcaagg
      661 cctcgtcccc gctcaggtgc gccatgatgc gcagctcgat ctggctgtag tcggcgctgg
      721 cgatgaaatt ccccggcggc gcaatgaagg cttcgcgcac gcgccgacct tcaggggtcc
      781 tgatcgggat gttctgcagg ttgggctcgt tgctggagag gcgccctgtc accgccactg
      841 cctgcgcata gtgggtgtgc acgcggccgg tgcgcggatg cgccagctgc gccagcttgt
      901 cggtgtaggt gcccttgagc ttggacagcc cccggtgttc cagcagcttg gccggcaggg
      961 ggtagtcctc ggccagcttc tccagcacct cttcgtcggt gctcgccacg ccgctgggtg
     1021 tcttcttcaa caccggcaag ccgagcttgg tgaacaggat gtcgccgatc tgcttgggcg
     1081 agcccaggtt gaagggctgg cccgcgagct cgtgcgcttc ccgctccaac tgcatgatgc
     1141 gctggcccag ttcggcgctt tgccgggcca gtatcgggcc gtcgatcagc acgccgttgc
     1201 gctcgatacg gtagagcgtt tcgctgctgc gcatttccag gtcgtagatg aagcgcaatt
     1261 tgctgtcggc ctgcagccgc ggccagagca ccagatggac gtccagcgtc tggtcggaat
     1321 cctcgcagga atattccgcc gccttgtcga cggacacctg cgcaaagccg atctggtgcg
     1381 cgcccttgcc ggcgatcgtt tcgtaatcga tgccgctgcg ccccaggtgg cgttcggcca
     1441 ggctggccag cccatgcggc ttgtggactt cgagcacgta gctttgcagc atcgtgtcat
     1501 gcgcgtagcc gcctacctcg atcccgtgat tggcaaacac gtggcggtcg tacttgacgt
     1561 gctggcccag cttgagcttg tccgggttct cgagccaggg cttcaggcgt gccagcactt
     1621 gatcgagggg caactggtcc ggcgcgtcgc cgtagctgtg ggccaacgga atgtaggcgg
     1681 cctcgcccgg cttgacgctg aaggacaggc cgatgatctg cgcctgcatc tcgtccagcg
     1741 aactggtttc cgtgtcgatg gcggcgagtt cggctgcttc cagccgagcc agccactcgt
     1801 cgaaacgctc ccacgtgaaa atcgtgtcgt attgcaggtt ggccgcttgc gagagacccg
     1861 acaggtccgg ctcctcgaac aggccggccg gtccggcacc cggacccacc ttcctcttgc
     1921 tgcggtgctc ctcgagcagt tcgggcggca cctcgtgggt ctcgagctgc ttgaccaggc
     1981 ccttgaatcc gtaccgatcg tagaacgcct tcagcgcctc ggtgtcctgg ccgccggtga
     2041 cggcaatgtc ttccagcgaa ggcaagcccg ggatgtggtc cttcagctcg caatctttct
     2101 tgatcgtgag caattcgcgg cccttgggca gccagtcgag cgctttgcgc aggttctcgc
     2161 cgacggcgcc tttcacctcg tggacgcggg cgatcagcgc gtcgagggag ccgtactcct
     2221 gcagcagctt gacggcggtc ttcgggccga ccttttcaac gcccggcacg ttgtcgaccg
     2281 cgtcgcccac cagggtctgg taatcgacca tcaggctggg cggcacgccg aactcggctt
     2341 cgacaccggc gacgtcgcgc ttgcggtcgt tcatggtgtc gatcaccgtg acgtgctcgt
     2401 tgacaagctg gctcaggtcc ttgtcgccgc tggagatcac ggtttcgatg ccgcgctcgg
     2461 tggccatgca cgagagcgtg ccgatcacgt cgtcggcttc cacgcccgga atgttcagca
     2521 ctttccagcc cagcaggcgc acgacctcgt ggatcgggtc gatctgcagg cgcaggtcgg
     2581 gcggcatcgg cgagcgggtg gccttgtagg ccgggtacag gctgtcccga aacgtcgggc
     2641 ccggcgcgtc gaacacgcag gccacgtagt cggcgcgtac gtccttgcgc agcttctgca
     2701 gcatgttgat catgccgcgg atggcgcccg tggccgggct ggcggggtcg cccggcacgg
     2761 cccgcaggtc gggcatggcg tggtaggcgc ggtacaggta gctggaaccg tcgaccagca
     2821 ataaggtctt ggggggctgg ctgttcgcgt tttgctcgtt agtcggctcg ctcatgagcg
     2881 gattgtgccg cgcccgggct tgacgcaatt catggatccc gggcttgacc cgggatccat
     2941 ccctgagggg ctgcacgcgc agcatggatt gcggatcaag tccgcaatga caaagagagg
     3001 aggaccgtca ttgacaaaga gaggaggact gtcattgcgg gaaatggatt tgtcatcccg
     3061 ggcttgaccc gggatccatg aaggcgcgag caagaagcag attcgtttct ttgccaacac
     3121 cccagcccag ccctgggctt aggccggaac aaacatccac cgcgcgtccg cctacaattg
     3181 ccccatgcac ccgcgcctca tgctcctcgc cccgctcctg gcctgcgcgg ccgtggcggc
     3241 ccagaccgtg ccgcccccgc aagcgccggc accggccgaa ggccgcaaga accagcgcat
     3301 cgagcgcatc catcacgagg atgcgggcgc gcggatcgac gagctgcgct acggcggcga
     3361 gacgcagagc atcgtggtcc agcccaaggc cgacgtaccg gaatacgaga tccagcccac
     3421 cgacttggcc cgcagccggc cggccgacca ccgcgacggc atgagcagcg ccaccggcca
     3481 gcgcgtctgg aacctgttca ggttctagat gtttgcggaa cgcgcttgaa tggccgtttt
     3541 cacggaggtc tccgagagcg aggctggcga gctggcgcac acgctccagc tgggccggct
     3601 gcgcgagctg cgcggcatcc cgggcggcat cgaaaacacc aactactttc tcacgaccga
     3661 ccagggcgac tatgtgctga cgctgttcga acgcctcacg ttcgagcagt tgccgttcta
     3721 cctgcacctg atgaagcacc tggcgcagcg cggcatcccc gtgccggagc cgcaggcgat
     3781 tcctggcagc gcttcagcgg gcgatccgga cggcgagatc ctgctcgagg tccgcggcaa
     3841 gccggcggcc gtggtggaca agttgcgcgg caagagcgag ctcgcgccga cggcggctca
     3901 ttgcgcggcc gtgggcgcca tggtcgcgcg aatgcacctg gccgggcggg actatccgcg
     3961 ccgccagccc aacttgcgcg gactcgcatg gtggaacgag accgtccccg tggtgctgcc
     4021 ctatgtggac gaggctcagg ccgccctgct tcgcagcgag ctggccttcc agaatcatgt
     4081 ggctgcgggc tcggcttatt ccgccctgcc gcgcggcgtc atccacgccg acctgttccg
     4141 cgacaacgcg ctgttcgagc agggccggct gaccgggttc ttcgatttct atttcgccgc
     4201 cgtcgacagc tggctgttcg acctcgccgt ttgcctcaac gattggtgca tcgaccttgc
     4261 caccggggcc cacgatgcgg atcgggcggc ggctttcctg cgcgcttatg ccggcgttcg
     4321 gcctctcgcc gcggccgaat ggcaattgct gcccgccatg ctgcgggccg gcgcgctgcg
     4381 cttctggatc tcgcgcctgt gggatttcca cctgccccgc gatgccgcga tgctccagcc
     4441 gcgcgatccg gtccacttcg agcgcgtgct gcgccagcgc gcggcccacc cggtagggct
     4501 ttgacgcaat gaaactcaac atcgttcccg cacgcactgg cgtccaatgg gtcaagctcg
     4561 gcatgaagac cttcgccaag cagccgctgg cgctggccgg gctgttcttc atgtacatgg
     4621 ccgccgtgat cgcggtggcg caggtgccgg tggtgggcgt ggtgatcggc ggcatgctgg
     4681 tgccggcggc cacgctgggc ctgatggccg ccacggcgga agcgacgaag gggcgctttc
     4741 ccatgccctc ggtgctgatc agcgcgtttc gcgcggggcg ccagcaagtg cgggccatgc
     4801 tgttgctggg cgtgatctac accgtcggct ccaagctgat gactgagctc gccaccctcg
     4861 tcatgggaac accggcagcc gattcaggcg aggtcgacgg ggcgacgctg ctggccctgg
     4921 tgctgcacac gccgctgttc ctgatgttct ggcacgcacc ggccctggtg cactggcacg
     4981 gggtcacgcc ggccaagagc ctatttttca gcatggtggc ctgcctgaag aacttcggtg
     5041 cgtttctcgt ttatggcctg acctggctcg tcgtgttcct cgcggtcggc atggtgttcg
     5101 gcttgatggg cgggctgctc ggcgggccgg agatggcgcg cgccgtgatg atgccgacgg
     5161 cgttgctgat ggccgccatg ttttccacct cgatctattt cacgtttctt gacagctttg
     5221 tcgccacgcc ggaagaaccc gaactacaac ctcccggaga gcccctttga cccagcacat
     5281 cctgcaaggc cgcagcgagg acgagatcct ctggttccgg cgccgcggct tccttcaggc
     5341 ggcggccgcc ttcagcgcga tgggcggcct ggccgccgcc caggcgcagc agcgcagcaa
     5401 tatcgtcgaa ctgcgcggtg acgtgctgct caatggcgag cgcctgcacg cgcaacggac
     5461 catccagacc ggcgacagcc tggagaccgg gcccggttcg cacctggtgt tcgtcatcgg
     5521 caactccgct ttccaagtgc gccagaactc gcgacttgtc gtggaacgcg gggccaccct
     5581 caacaccgtg agcgtgctgc ggatgatcac gggcgcggtg gccagcgtct ggggccgcgg
     5641 caccagccgg cagatcgtca cgcccacgct caccgccggt atccgcggca ccggcgttta
     5701 caccgaggtc ttcccgcagc aaggctatcg cagctatttc tgcaactgct atggcacggt
     5761 ggatgtggac gccggtggcg acaagacggt ttcgcgcgcc gagtaccacc agtcgttctg
     5821 gggcgaagtc gagccgaaaa acggccgcta cctgacgccg gccaggatga tcaatcacac
     5881 cgatgaagag ctggagttgc ttgcccggct ggtgaaccag cgcaccgcct ggcagatcgc
     5941 gggcaggaag ggcgcggcgg acggcagcgg gtatatgaaa taagtgagtc aagcgcgcaa
     6001 cgacaggtcc ctgtgtctgt catcccggac cccggatcaa gcctgccccg gacctgatcc
     6061 ggggttccgg ggcaggcccg atccggggat cctgacttct gaattcatgg attgcgggtc
     6121 aggcccgcaa tgacaagggg aggctgtcat gccggacttg atccggcatc cacctctggt
     6181 ttgtcatccc gggcttgacc cgggatccat ggattgcggg gcacgctagt ccgcaccgcc
     6241 tgacccctgc agtcgtttgg ccaatgccac caggggcggc cggaccttgt ccagcaggaa
     6301 ctcgcgcgac agattgaagc tcgggccgcc gcagttgatc gccatgatcg accggccgcc
     6361 gggcggccgg aacgcaatgg cgatggcgtt cacgtccttc tgccactccc cgaaggaagt
     6421 gctgcagccg agttcccggt actcggccag tgcgcgggct atgccttcgc gcacgcgcgg
     6481 ccatgccgcc tcgtcccgtt cgcggatgcg tttgagcagg ttgtcgcggt cggcttcgcc
     6541 gcattgcgcg aggtaggccc ggcccatggc ggtggtcgcc agcggaatgc gcgagcccac
     6601 gtccaggctc agcgtcaagg ccgcctcgct gcggcagttc tcaacataga tcatgctgag
     6661 ccggtcgcgc atgcccagcg agaccatcgc atgcgagtgg tcggccagtt cctgcatcag
     6721 cggccgcgcg atctggcgca tgtccatgcg cgccagcatg gtgctgccca gcgcgagcac
     6781 ggcactgccc aggcggtagc cggtgttccc gctgccgtcg tcggcgtgct ccaggtaccc
     6841 gtgcttggtc agcgtgtagg tcaggcgtga aatggtggac ttgggcaggc cgcagcggcg
     6901 cgacaactcc tggttgccca gcatgcgatc gcgcgaacgg aaggcgctca gcacgtccag
     6961 gccacgtgcc agcgcggtga cgaagtgacg atcggccttg gcgttaatcg gtcggcgcgg
     7021 cgggcgcgcg acggtgtggg gtgaggatgg aatcgccatc gtgttctgca tcatggaatc
     7081 ggcactcctt gcagctggtg attatttcat aggcgcgatc gcggattacg gccaagctgg
     7141 ctggtggaat cgaacttcct ggattgcggg tcaagcccgc aatgacaagg aaaaggctgt
     7201 catcccggac cccggatcaa gtccggggca ggcccgatcc gggatccatg aattcaggag
     7261 tcatggattg cggggccgga ccggagcgtg atgcctcggg acgtgcatag cgtaggcccg
     7321 gcattgggct cgctgcactg gacagcttcg cgccccgggc gttccgcgag cccagcccgc
     7381 aatgacaaag ccaaatgatt ccgccttgca gaacttcgcc tgcgcgcgcc gcggcttcaa
     7441 gtcaccggcg tgagcttggc gaccgacagg gccagccatt tgacgccatg ccgggggaaa
     7501 ttgacctgcg cccgcgcgtc atcgccctgc ccttcgatcg acatgacggt gccctctcca
     7561 aacttggtgt ggaagacctt catgtcggca cgcaagccat gcgagggcga cttgcgcgcc
     7621 ggcaccggcg gactcgcgaa cacgtctttg ccccggctgc ccgcggtggc gccgcgggac
     7681 gtggcatagc ccccgccgaa accgaaggcg gagccgacga acttctggtt tttcggcgtc
     7741 agccacttca tggcgccctc gggcaactca tcgaaaaagc ggctcttgac gttgtagcgg
     7801 gtctggccgt gcagcagccg tgtctgcgcg tggctcaggt acaggcgctt gcgcgcacgc
     7861 gtgatggcga catacatcag gcggcgttct tcctccagcc cctcgtggtc ggacatggaa
     7921 ttttcgtgcg gaaacaggcc ctcctccagg ccgctgatga agacgcagtc gaattccagc
     7981 cccttggccg aatgcaccgt catgagctgg atggcgtcct gtccggcctg ggcctggttg
     8041 tcgccggact ccagcgcggc gtgggtgagg aacgccgcca gcggcgacaa ggtttcgccc
     8101 gtctcggcat cgggcggcac cccggatcgg agtccggggc gggctccatc gggtgccgcg
     8161 ccctcacccc cgccctctcc cggagggaga gggagcaact cccttccccc tctcccgcgc
     8221 tctcccggag ggagagggag caactccctt ccccctctcc cgtttgcggg agagggttgg
     8281 ggtgaggggg cattggggtc caggccctgg cttacggggc tctggctcag ttcgtccacc
     8341 ggcagcgcaa cggcatcgcg cccgaatccc tcctgcgtga cgaaactctc ggccgcgttg
     8401 accagttcct ccaggttctc gatgcggtcc gcgccttcgc gctcactctt gtaatgctcg
     8461 atcagcccgg acgcctgcag caccagctcg atgatctcgc gcagcgtgag gttcaaggtc
     8521 tgctcacgca gcgcgtcgat cttcgccacg aacgccgtga ggttggtccc cgccttgccc
     8581 gtggtcgcgc tcacggcatc gtgcagcgag cagccggccg cccgcgccgc gtcctgcaac
     8641 tgctcgatgc tgcgtgcccc gatgccgcgc ggcggaaagt tcacgatgcg caggaagctg
     8701 gtgtcgtcgt gcgggttctc cagcaggcgc aggtacgcca gcgcatgctt gatctcggcg
     8761 cgttcgaaga atcgcaagcc gccatagacg cggtacggca tggcggcatt gaacagcgct
     8821 gtttcgatca cccggctttg cgcgttgctg cgatagagca ccgcgatctc gtggcgctcg
     8881 tacccgcccc ggggaccatc gccgcgcacc agg
//
LOCUS       DASWIY010000364         3720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_987500, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000364 DASWIY010000000
VERSION     DASWIY010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987500"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VGA59_13000"
     CDS             complement(<1..267)
                     /locus_tag="VGA59_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9720639.1"
                     /translation="MRRLLVTLALSGAAFAASAAFPDKPITLVIPYPPGGAVDVTGRI
                     IARGLESQLGQPVIVDNKPGAGTVLAAGALARAPADGYTLLLSGN"
     gene            430..1599
                     /locus_tag="VGA59_13005"
     CDS             430..1599
                     /locus_tag="VGA59_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX9720640.1"
                     /translation="MYVGKTLFAQVMEFVPWTSFARIVHRYGGNAGVRTLSCGEQFRA
                     MAFAQLTWRESLRDIEVSLSANAPKLYAMGFRAAVKRSTLADANESRDWRIWFDLATL
                     LIRRARKLYASDSLGVELDNTVYALDSTTIDLCLSLFDWAPFRSTKAAIKLHTLLDLR
                     GSIPAFIHISDGKLHDVNVLDMLSFEAGAFYVMDRGYVDFERLYALHQAGAFFVTRAK
                     SPMDARRVYSAPVDREAGVICDQRVMLNGYYSAKKYPEHLRRVRFKDPESGKTLVFLT
                     NNTALPALTIAALYKSRWQVELFFKWIKQHLRIKKFLGTSENAVKTQVWCAVATYVLI
                     AIVKKELHLDASLYTCLQILSVSVFEKTQLSCALQLDDSGTELTLDDNQLILFNF"
     gene            1642..2130
                     /locus_tag="VGA59_13010"
     CDS             1642..2130
                     /locus_tag="VGA59_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9720641.1"
                     /translation="MIRKLHHNAYRCRDSEETRRFYENFLGLPLAGTLEIKETKSGRK
                     TETLHTFYRLDDGSYLAFFEAPDMPFEFKAQHDFDLHIALEVEEPSLHAMLEHGKSQG
                     METRGISDHGFIRSIYFRDPNGYVIELTSKQPGHDEAMDPAVNGARAKLDAWQAAKHQ
                     GQ"
     gene            2212..2288
                     /locus_tag="VGA59_13015"
     tRNA            2212..2288
                     /locus_tag="VGA59_13015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2246..2248,aa:Arg,seq:tct)
     gene            2448..2987
                     /locus_tag="VGA59_13020"
     CDS             2448..2987
                     /locus_tag="VGA59_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720642.1"
                     /translation="MLQFWLRWIFAFSLTLTALTPSFAASTAKEALKDVTAAAQKWQP
                     DAVLTHISTLMGRGDGKADQWLYTFYSPKAKKSAIVTARDKTVANVEADVRNTSTDPL
                     GGDYVDSDKAVEAATKAGLKLDKGAKGLGMGLVVGNQAVGKPQLFWSVTIMSGDGMSS
                     VLMNGKDAAFIRRNDHKFK"
     gene            complement(3038..3388)
                     /locus_tag="VGA59_13025"
     CDS             complement(3038..3388)
                     /locus_tag="VGA59_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720643.1"
                     /translation="MKRAAAVEISPCDPPVLGDLSLEPFSPHDWSKAMDVVAWLTEQA
                     GRLAPRGAGEAAMSEAFSAAFGHMVAYFHHVTLDGDLPREALAKAAAGTMELTPEQRD
                     FVRAASSTRGLNKV"
BASE COUNT          720 a         1149 c         1151 g          700 t
ORIGIN      
        1 attgccgctc agcagtagtg tgtagccatc cgctggcgcg cgcgccaacg cgccggcggc
       61 cagcacggtg ccggcacccg gtttgttgtc cacaatcacg ggctggccca gctgcgactc
      121 gagcccacgg gcgatgatgc ggcccgtgac atccacggct ccgccgggtg gatagggaat
      181 gacgagggtg atcggtttgt ccggaaaggc cgcagatgcg gcgaatgcag ccccgctcag
      241 ggccagcgtc acgagcagtc ttcgcatagt tcaagtctcc atctcactgc tgtccggtta
      301 aatgacatgc gccgagcgga agatcaagca ttggcgcggc ttgtaggctg attttgtttg
      361 gtgccgactt gaacttctct tgccaaaaac cgcgtacttt cccggggttg actgccccga
      421 ggaatgcaca tgtatgttgg caagaccctg ttcgcccagg tcatggagtt cgtgccgtgg
      481 acgagctttg cgcgtatcgt gcatcgctac ggcggcaacg cgggcgtgcg cacgctgtcg
      541 tgtggcgagc agtttcgcgc catggctttc gcgcagctga cctggcgcga gagcctgcgc
      601 gacatcgagg tcagcctgtc ggccaacgcc cccaagctct acgcgatggg ctttcgcgca
      661 gcggtcaagc gctccacgct ggccgacgcc aacgaatcgc gcgactggcg catctggttc
      721 gatctggcca ccttgctgat ccgccgcgcg cgcaaacttt atgcgagcga ttcgctcggt
      781 gtcgagttgg acaatacggt gtacgcgctg gactccacca ccatcgatct gtgtttgagc
      841 ttgttcgact gggcgccctt tcgctccacc aaggcggcca tcaagctgca cacgctgctg
      901 gatctgcgtg gctcgattcc ggcgttcatc cacataagcg acggcaagct gcacgacgtg
      961 aacgtgctgg acatgctcag cttcgaggcc ggtgccttct acgtgatgga ccgcggctac
     1021 gtggacttcg agcgcttgta cgcgctgcat caggccggcg ccttcttcgt cacgcgtgcc
     1081 aagtcgccga tggatgcgcg gcgggtctac tcggcacccg tcgatcgcga ggccggtgtg
     1141 atctgcgacc aacgcgtgat gctcaatggc tactattcgg ccaagaagta tcccgagcat
     1201 ctgcggcgcg tgcgattcaa ggaccccgag tcgggcaaga cgctggtgtt cctgaccaac
     1261 aacacggcgc tgccggcatt gaccatcgcc gcgctgtaca agagccgttg gcaggtcgag
     1321 ctgttcttca agtggatcaa gcagcatctg cgcatcaaga agtttctggg caccagcgag
     1381 aacgcggtga agacgcaggt gtggtgcgcc gtggccacct acgtgctcat cgccatcgtc
     1441 aagaaggagc ttcacctgga tgcctcgctc tacacatgtc tacagatctt gtcggtctca
     1501 gtcttcgaga aaacccagct ttcatgcgcc ttgcagctcg atgactctgg aaccgaacta
     1561 acactcgacg ataaccaatt gattttgttc aacttttaac cggacagcag tgagtccttc
     1621 gaaacgatct gaggtgccgc catgatcagg aaactccatc acaacgccta ccgctgccgc
     1681 gactctgaag agacccgccg gttctacgag aacttcctgg gcctgccgct ggccggcacg
     1741 ctggagatca aggaaacgaa aagcggccgc aagaccgaaa cgctgcacac cttctaccgc
     1801 ctggacgacg gctcctacct ggcctttttc gaggcgcccg acatgccctt cgagttcaag
     1861 gcgcagcacg atttcgacct gcatatcgcg ctggaagtgg aggagccttc gctgcacgcc
     1921 atgctggagc acggcaagag ccaaggcatg gaaacgcgcg gcatctccga tcacggcttc
     1981 attcgctcga tctatttccg cgacccgaac ggctacgtga tcgagctcac cagcaagcag
     2041 ccgggccacg acgaggcgat ggacccggcc gtcaacggcg cgcgtgccaa gctcgatgcc
     2101 tggcaggccg ccaagcatca gggccagtga gccactcaca ccgtcccggg gcggcgggtg
     2161 aggaagaagg cgggcactgg gcggcgcagt tatcattccg cgcatgctca tctgcccgta
     2221 gctcagttgg atagagcacc ggccttctaa gccggttgtc gggggttcga ttccctccgg
     2281 gcaggccaat taagataaca aggactcgcc cggccaatcg ggcgatttgg ttcgctgatc
     2341 cttggccttg gcgcctgaaa ggacgccctt tcggtttgta ccaacaaaga cttgtgggca
     2401 ctttcttcaa agccgatgca cacctggact caggaaagga aacagccatg ttgcaattct
     2461 ggctgcgctg gatttttgcc ttctcgctga cactcacggc actgacgccg tcgtttgctg
     2521 catccacagc caaggaagcg ctcaaggacg tgacggctgc ggcccagaag tggcagcccg
     2581 acgccgtgtt gacgcatatc tcaacgttga tgggtcgggg cgacggcaag gccgaccaat
     2641 ggctttacac gttttactcg ccaaaagcga agaagagcgc catcgtgact gcgcgcgaca
     2701 agacggtggc gaacgtcgag gccgacgtgc gcaacacgtc aaccgatccg ctgggcggcg
     2761 actatgtcga cagcgacaag gcggtcgagg ccgccaccaa ggccggcctg aagctggaca
     2821 agggcgccaa agggcttgga atggggctgg tagtcggtaa ccaggcggtc ggcaagccac
     2881 agttgttctg gtcggtgacg atcatgagcg gagacggcat gtcctccgtg ctgatgaacg
     2941 gcaaggacgc agccttcatc cggcgtaacg accataagtt caagtgatgc agttgaatgc
     3001 agcgacttga atgaggcggc caatcagcgg cttcggctca aaccttgttc agcccacgcg
     3061 tgctggaggc cgcgcgaaca aaatcccgtt gctcaggcgt cagctccatc gtccctgccg
     3121 cggctttggc aagggcctca cgcggcagat cgccgtccag ggtgacatgg tgaaagtagg
     3181 ccaccatatg gccgaaagcg gcactgaaag cttccgacat ggccgcctcg cccgcacctc
     3241 tgggagccaa cctgcctgcc tgctccgtca gccacgcgac gacgtccatg gccttgctcc
     3301 aatcgtgcgg gctgaaaggc tccagcgaca gatcgcccag gacagggggg tcgcacggcg
     3361 aaatctcgac tgcggcggca cgtttcattt ttcgctccct tcaggattct ggtgcgccca
     3421 gattaggtgc gctcaggagc gtgggaaagg gacaggttgc tcaatctatc tgggacaaac
     3481 gtcccgggcg ggccacgcgg ttgatcggag ccgtcaggct cgcgtgctgc cccgggaagg
     3541 tgcccccaac gccttctcag tggtccatta cgctgcgcgc cacccgcgac gcatgaaacg
     3601 ctgcggtcag tgcgggggcc accgcggaac cggctttgcc gggccgctgg tggcgccccc
     3661 ttgaggggga ggcgccgaag gcgcttcggg ggtgggacat cagtaccgct ccatctcggg
//
LOCUS       DASWIY010000365         5020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_618117, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000365 DASWIY010000000
VERSION     DASWIY010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618117"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1379)
                     /locus_tag="VGA59_13030"
     CDS             complement(6..1379)
                     /locus_tag="VGA59_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9720644.1"
                     /translation="MSQLEIRNPANGEKIAAIAADDGPSVAAKARRARAAQPAWAALP
                     LQQRKDCIGRFREGVVRDLESLAVTMTRETGKPIRMSRNELSGLLGRLDFFVGMVEPV
                     TATETVFDEGGMTERIEHVPLGLVGNISAWNYPWFVGCNVIVPALLTGNAVLYKPSEY
                     ATLTGLEIARLLHAAGVPEDVFAALVGAGPVGAALLQQPIDGLFFTGSHATGSRIAQA
                     VGQRFMKLQLELGGKDPTYVCDDADPKAAAESLADGAMYNTGQSCCSVERIYVHEKIY
                     DTFVAAFVREVQGFKAGDPMSEDTYIGAITRAPQLDVLDAQVADAKAKGAMLHTGGHR
                     LPGPGNWYAPTVFSNVDHGMALMRQESFGPIVGIQKVSNDEDAVRLMNDTRYGLTAGV
                     YTPNGERARALLARVHAGTLYWNCCDRVSPRLPWSGFGDSGVGLTLSTYGIQAFTRPR
                     AWHLRRP"
     gene            complement(1376..2194)
                     /locus_tag="VGA59_13035"
     CDS             complement(1376..2194)
                     /locus_tag="VGA59_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX9720645.1"
                     /translation="MANGIPLDGASRLKIGISACFMHADPARPLFTGKTLQYVEQSIA
                     HWVMSTGALAVMIPSPTGATQRGDVTLDHYAQWLDALVLHGGADVSPRSYGEEPLQER
                     WAGDRIRDEYEIDLVAAFERQGKPVFGVCRGLQLLNVAYGGTLYQDIPTQRPEALQHR
                     DAVVYDLNFHTVDILAGSRLASLYPELPRVKVNSIHHQAIKDLAPGFTVEAVSNEDGL
                     IEAIRRDDAAKPYLAAVQWHPEFHQPFSDTIDDAALLEDFLTAARAARKDAPLP"
     gene            complement(2201..3334)
                     /locus_tag="VGA59_13040"
     CDS             complement(2201..3334)
                     /locus_tag="VGA59_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX9720646.1"
                     /translation="MNITGFSFPTSIRFGPGARKEVAEHLLGQGLKRPLIVTDKRVAG
                     LPVLSEFKTHLAGMNVAVFGEVFGNPTASQVMEGAAAFRAHEADCVIGFGGGAALDVA
                     KVVGLAATHEGDILEYAWDHPKVRSIDKPLPYFVALPTTSGTGSEVGRSSVVSENETH
                     LKRVIFSPKILARTVFADPELTLDLPPQVTAATGMDALTHNIESYLSPAYHPLCDGIA
                     LEGARIAAGALVTAVHESANLQARSDMMMASMMGAIAFQKDLGAVHSCAHALGAMCDL
                     HHGLANALMIDTVMAWNHEAVPRKFDELAHVCKVWGGGAEFVPWLKDLKARIGLAGKL
                     SAHGVREGQIPRLVEIAVADICHQTNPRPCTAADFKRLFEKAL"
     gene            complement(3591..>5020)
                     /locus_tag="VGA59_13045"
     CDS             complement(3591..>5020)
                     /locus_tag="VGA59_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX9720647.1"
                     /translation="AIYYLFGNLPLLGQYSGIDNVPAISVAGWVFDSGRKSYLLIWIA
                     AMAALVAAQNLLDSRCGRAIRALRFRAAMAESCGVDTAALKQVVFIQAALLAGVAGWL
                     HAHFLRFVSPHAFNVNAGIDYLFMAVIGGASSVWGALVGASIMTVAKEWLKDLLPQVF
                     HASGNYETIVFGVLMILLLRRTREGLVPALSRWWPSSWRLARPPRSAAQLDAAPMSRR
                     ERPAAGTPLLEVSGMQKRFGGLVAVKDMSFSMRAGEILGLIGPNGAGKTTMFNLISGA
                     LPASGGEVRLLGERIDGHPSRDIARRGLARSFQHVHLVSRMSVLDNVALGAHLRGAKG
                     TLSAMLRTDRGEEARLLAEAARQIERMGLGEAMHESAGSLPLGRQRVVEIARALAADP
                     LLLLLDEPAAGLRYAEKQALSELLRRLRGEGVTILLVEHDMEFVMGLVDRLVVMDFGE
                     KIAEGLPQEIQRHPAVIEAYLGGVE"
BASE COUNT          789 a         1726 c         1706 g          799 t
ORIGIN      
        1 gccgctcagg gccggcgcag gtgccaggcc cgcggccggg tgaaggcctg gatgccgtag
       61 gtcgagagcg tgaggcccac gcccgaatcg ccgaagccgc tccagggcag gcgcgggctg
      121 acgcggtcgc aacagttcca gtacagcgtg ccggcatgga cgcgcgcgag cagcgcgcgg
      181 gcgcgctcgc cgttgggcgt gtagacgccc gccgtcaggc cgtagcgcgt gtcgttcatg
      241 aggcgtaccg cgtcctcgtc gttcgagact ttctggatgc cgacgatggg gccgaagctc
      301 tcttgcctca tcagcgccat gccgtggtcg acgttgctga acacggtggg cgcgtaccag
      361 ttgcccggcc ccggcaggcg gtggccgccg gtatgcagca tggcgccttt ggccttggcg
      421 tcggctacct gggcgtcgag cacgtccagc tgcggggccc gggtgatggc gccgatgtaa
      481 gtgtcctcgc tcatgggatc gccagccttg aagccctgga cctcccgcac gaacgcggcg
      541 acgaatgtgt cgtaaatctt ttcgtgcacg tagatgcgct cgaccgagca gcagctctgg
      601 ccggtgttgt acatggcgcc gtcggccagc gattcggccg cggccttcgg atcagcgtcg
      661 tcgcagacat aggtcgggtc cttgccgccc agttccagtt gcagcttcat gaagcgctga
      721 ccgaccgcct gcgcgatgcg cgacccggtg gcatgcgagc cggtgaagaa caggccgtcg
      781 atgggctgct gcagcagcgc ggcgccgacc ggccccgccc ccaccagcgc ggcgaaaaca
      841 tcctcgggca cgccggccgc gtgcagcagg cgcgcgatct ccagcccggt gagggtcgcg
      901 tactcggacg gcttgtacaa cacggcgttg ccggtgagca gcgccggcac gatgacgttg
      961 cagccgacga accagggata gttccaggcc gagatattgc ccaccaggcc gagcggcaca
     1021 tgctcgatgc gttccgtcat gcccccttcg tcgaagaccg tttcggtcgc agtgaccggc
     1081 tcgaccattc ccacaaagaa gtccaggcgc ccaagcaggc cgctcagttc gttgcgggac
     1141 atcctgatcg gcttgcccgt ttcgcgcgtc atcgtcaccg ccagcgattc gaggtcgcgc
     1201 accacgcctt cccggaagcg cccgatgcag tccttgcgct gctgcaacgg gagcgctgcc
     1261 cacgccggct gggcggcgcg cgcgcgccgc gccttggcgg ccaccgacgg cccatcgtcg
     1321 gcggcaatcg cggcgatctt ctcgccgttc gcggggttgc ggatctcgag ctggctcatg
     1381 ggagcggcgc gtccttcctg gcggcgcggg cggcggtgag gaagtcctcg agcagggccg
     1441 cgtcgtcgat ggtgtcggaa aaaggttggt gaaattcagg gtgccattgc accgccgcca
     1501 gataaggctt ggcggcgtca tcgcgcctga tcgcctcgat caggccgtcc tcgttgctca
     1561 ccgcctccac cgtgaagccg ggcgcgagat ccttgatcgc ctggtggtgg atgctgttga
     1621 ccttgaccct gggaagttca gggtacaggc tggccagccg cgagccggcc aggatgtcca
     1681 cggtgtggaa gttgaggtca tagacgacgg catcgcggtg ctgcagggcc tcgggccgct
     1741 gcgtcgggat gtcctggtag agggtgccac cgtaggccac gttcagcagt tgcaggccgc
     1801 ggcagacgcc gaacaccggc ttgccctggc gctcgaacgc ggccaccagg tcgatctcgt
     1861 actcgtcgcg tatccggtcg ccggcccagc gctcctgcag cggctcctcg ccgtagctgc
     1921 ggggcgaaac atccgcgccg ccgtgcagca ccagagcgtc cagccattgc gcgtagtggt
     1981 ccagcgtcac gtcgccgcgc tgggtggcgc ccgtgggact gggaatcatg acggccagcg
     2041 ccccggtgga catgacccag tgtgcgatcg actgctcgac gtactgcagg gtcttgccgg
     2101 tgaacagcgg gcgtgccggg tcggcgtgca tgaagcaggc cgaaatgccg atcttcaggc
     2161 gcgaggcgcc gtccagggga atgccgtttg ccatcatctg ctacagggcc ttttcgaaga
     2221 gccgcttgaa atcggcggcc gtgcaaggcc gcgggttggt ctggtggcag atgtcggcca
     2281 cggcgatctc caccaggcgc ggaatctgcc cttcccgcac tccgtgcgcg gacagcttgc
     2341 cggcgaggcc gatccgcgcc ttcaggtcct tcagccaggg cacgaattcg gcgccgccgc
     2401 cccagacctt gcacacgtgc gccagctcgt cgaacttcct gggaacggct tcgtggttcc
     2461 aggccatgac ggtgtctatc atcagcgcgt tggccaggcc atggtgcagg tcgcacatcg
     2521 ctcccagcgc atgggcacag gagtgaaccg cgcccaggtc tttctggaag gcgatggcac
     2581 ccatcatcga ggccatcatc atgtcgctgc gcgcctgcag gttggcgctc tcgtgcaccg
     2641 ccgtgaccag cgcgccggcc gcgatgcgcg cgccctccag cgcgataccg tcgcacaagg
     2701 ggtggtaggc cggcgacagg tagctctcga tgttgtgggt cagcgcgtcc atgccggtgg
     2761 cggccgtgac ctgcggcggc aggtccaggg tgagctcggg atcggcgaac accgtcctgg
     2821 cgaggatctt cgggctgaag atcacgcgct tcaggtgggt ctcgttttca ctcaccaccg
     2881 aggagcggcc cacttccgag ccggtgcccg aagtcgtggg caaggcgacg aaatacggca
     2941 agggcttgtc gatcgagcgc accttgggat ggtcccaggc atattccagg atgtcgccct
     3001 cgtgcgtcgc ggcgaggccg accaccttgg ccacgtccag cgcggcaccg ccgccgaagc
     3061 cgatcacgca atccgcttca tgcgccctga acgcggccgc gccttccatc acctggctgg
     3121 cggtcggatt gccgaagacc tcgccgaaca ccgccacgtt catgccggcg agatgcgtct
     3181 tgaattcgga cagcaccggc aggccggcca cccgcttgtc agtgacgatg agggggcgct
     3241 tgaggccctg gcccagcagg tgctcggcca cctccttgcg cgcgcccggc ccgaagcgga
     3301 tgctggtggg aaaggaaaag cccgtgatgt tcatagtggc tccagttgct ccacctcgct
     3361 gcgtcccacc cgcaacgtac gacatcgcgg gatcaatgag tgggccaccg cggaaccggc
     3421 tttgccgggc cgctggtggg ccttgcaggc cgcatggccg cgagacgcgg ccattcctcg
     3481 acctgtccaa accgacgcga gtcggtttgg agccgcaggt ctcggccccc ttgaggggga
     3541 gagggcgaag cccgaggggg catcgccgaa ggcacttcgg gggtgggctt tcattccact
     3601 cccccgaggt aggcctcgat caccgccggg tgccgctgga tctcctgcgg caggccctcg
     3661 gcgatctttt cgccgaagtc catcaccacc agccggtcca ccaggcccat cacgaactcc
     3721 atgtcgtgct cgaccagcag gatggtgaca ccctcgccac gcagccgccg cagcagctcg
     3781 gacagcgcct gcttttccgc gtagcgcagg ccggcggcgg gctcgtcgag cagcagcaag
     3841 agcgggtcgg cggccaaggc ccgggcgatc tcgaccacgc gctggcgccc cagcggcaag
     3901 ctgccggcgc tttcgtgcat cgcttcaccc aggcccatgc gctcgatctg gcgcgcggct
     3961 tcggccaaca accgggcctc ttcgccacgg tcggtgcgca gcatcgcgga caacgttccc
     4021 ttcgcgccgc gcaggtgcgc gccgagcgcc acgttgtcca gcaccgacat gcgcgagacc
     4081 aggtgcacat gctggaaact gcgcgccagg ccgcggcgcg cgatgtcgcg cgaggggtgc
     4141 ccatcgatgc gctcgcccag caggcggacc tcgcccccgc tcgcgggcaa tgcaccgctg
     4201 atcaggttga acatcgtcgt cttgcccgcg ccgttgggcc cgatcaaccc caggatctca
     4261 ccggcgcgca tcgagaacga catgtccttg acggccacca gaccgccgaa gcgcttctgc
     4321 ataccgctca cttccagcaa cggcgtgccg gccgccggcc gctcgcgccg cgacatcggc
     4381 gcggcgtcca gctgcgctgc gctgcgcggc gggcgcgcca gccgccacga tgacggccac
     4441 cagcgcgaca gcgccggcac cagaccctcg cgcgtgcgcc gcagcagcag gatcatcagc
     4501 acgccgaaca cgatcgtctc gtagttgccg ctggcatgga acacctgcgg cagcaggtcc
     4561 ttcagccatt ccttggccac cgtcatgatc gacgcaccga ccagcgcgcc ccagacgctg
     4621 ctggcgccgc cgatgacggc catgaagagg tagtcgatgc cggcattgac gttgaaggca
     4681 tgcgggctga cgaagcgcag gaaatgcgcg tgcagccagc cggcgacgcc ggccaacagg
     4741 gccgcctgga tgaacacgac ctgcttcaat gccgcggtgt cgacgccgca gctttcggcc
     4801 atcgcggcgc ggaagcgcag cgcgcgaatg gcgcgtccgc agcgcgagtc gagcaggttc
     4861 tgcgccgcca ccagcgcggc cattgcggcg atccagatca gcaggtagga cttgcggccg
     4921 ctgtcgaaga cccagcccgc cactgaaatt gccggcacat tgtcgatccc gctgtactgg
     4981 ccgagcaggg gcaggttgcc gaacaggtag tagatcgcga
//
LOCUS       DASWIY010000366         3900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_989862, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000366 DASWIY010000000
VERSION     DASWIY010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989862"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VGA59_13050"
     CDS             <1..1081
                     /locus_tag="VGA59_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal
                     protein"
                     /protein_id="HEX9720648.1"
                     /translation="SDYPPEAVEAWQGPRSDMDDVRRWILSYAILAPHSHNLQSWMAD
                     LRQPGEITLYCDLTRLLPETDPFSRQIMMSHGTFLELLDLAARRKGLRAQIELFPQGE
                     FGPAQLDKRPVARIRLAPDAGIKPDPLFDQILRRRTNREAYEPREPAPAALQAIAASA
                     SGFPMRTGFAGADQPEALQKHRAIASEAWRIELVTPRTILESYKVLRVGPREIAQHRD
                     GLSINAPMARALTALGLFDRSKAPGPDDMATTSQLKDFNTKIAGTPAFYWLVTEGNDR
                     KTQVNAGRAFARAQLAATAHGLSMQPLSQALQEYPEQVRPYADIHALLQAPPPRYTVQ
                     MWSRLGYAPPVGPAPRRPLGDMIVA"
     gene            complement(1021..1545)
                     /locus_tag="VGA59_13055"
     CDS             complement(1021..1545)
                     /locus_tag="VGA59_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX9720649.1"
                     /translation="MTSTTRLAQLLQDRGWTLATAESCTGGLIAAACTELPGSSNWFE
                     RGFVTYSDESKVELLGVDAASIVAHGAVSEAVVRAMARGAIRHSPARVSVAVTGIAGP
                     AGGSKEKPVGTVWFGFMVDGLLTSEMKRFDGDRAAVRRDTVEHALARLVELLDQATIM
                     SPNGRRGAGPTGGA"
     gene            complement(1542..2072)
                     /locus_tag="VGA59_13060"
     CDS             complement(1542..2072)
                     /locus_tag="VGA59_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX9720650.1"
                     /translation="MTTPLNPPLRPAVMRPSVRFMLSHPAHCIALGFGSGLSPVAPGT
                     AGTLWAWLAYALMQRWLSPAAIGLVIAAALAIGWWACTVTAAHMHVLDPGSIVWDEVI
                     AFWIVLWLVMPAGLWAQLGAFLVFRVFDAVKPGPVAWADNLFHGFGWRGGFGIIFDDL
                     VAAFCTLLVIAAWRFL"
     gene            complement(2069..3007)
                     /locus_tag="VGA59_13065"
     CDS             complement(2069..3007)
                     /locus_tag="VGA59_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9720651.1"
                     /translation="MVTIRPMRDLQFDDVHLFSRVAELGTLSAVARERDVPVSQVSRT
                     LSRIEKACGARLIHRSTHGLSLTPEGETFRDYCRRIVGTLDELEGEFASKAREVSGLV
                     RVATSTVVAQYQLVPSLPGLNSRHPRLRVELEVGDRLLDMVRDGIDIAIRTVSNLPET
                     AVARQLGTLGRALYAAPSYVASAGLPQLPESLREHRLIANSNVAMLNQWPFIVNGKAF
                     VFTAEGHWRTNDTNMAATMVLQGLGIGRLATLVGERLVKQKLLVPVLEKFVDPGPVPV
                     YAVTAPSRHRLPKIKACIDYWAEWFAKSGDAAAGKP"
     gene            3125..3340
                     /locus_tag="VGA59_13070"
     CDS             3125..3340
                     /locus_tag="VGA59_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720652.1"
                     /translation="MTTRNRTDCSYYDMIDAAKLRAERLRDEAISEFWRGTGEAARRA
                     ERSARRFASSLARHARLRAQSRACPAP"
     gene            complement(3378..>3900)
                     /locus_tag="VGA59_13075"
     CDS             complement(3378..>3900)
                     /locus_tag="VGA59_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX9720653.1"
                     /translation="LPRDVGWAMTFFQCIAAATLLCALTHSARAGSFEGVVTHVTDGD
                     TLWVRPAGGGAPRKIRLQGIDAPESCQVHGTASRDALAARALHQRVKVASHLRDDYQR
                     ALGNVSQGGQDLGLWMVSRGHAWSYRFRRDPGPYAREEAQARRAGLGLWSGHAPVRPR
                     DFRKRHGSCGTDS"
BASE COUNT          651 a         1387 c         1305 g          557 t
ORIGIN      
        1 cagcgactac ccgcctgaag cggtcgaagc ctggcaaggc ccgcgcagcg acatggacga
       61 tgtgcgccgc tggatcctga gctacgccat cctggcgccg cattcgcaca acctgcagtc
      121 ctggatggcg gacctgcgcc agccgggcga gatcacgctg tactgcgacc tcacgcggct
      181 gctgcccgag accgatccct tctcgcgcca gatcatgatg agccacggca ccttcctcga
      241 actgctcgac ctggcggcgc ggcgcaaagg actgcgcgcg cagatcgagc tgtttccgca
      301 aggcgaattc ggccccgccc agctcgacaa gcggccggtg gcccgcatcc gcctggcgcc
      361 cgacgccggc atcaagccgg acccgctgtt cgaccagatc ctcaggcgtc gcaccaaccg
      421 cgaggcatac gagccgcgcg agccggcgcc ggccgcgctg caagccattg ccgccagcgc
      481 gtccggcttc ccgatgcgta ccggtttcgc cggcgcggac cagcccgagg cgctgcaaaa
      541 gcacagggcc atcgcaagcg aggcctggcg catcgaactg gtgacgccgc gcaccatcct
      601 ggagtcgtac aaggtgctgc gggtcggccc gcgcgagatc gcgcagcatc gcgacggctt
      661 gtcgatcaac gcgcccatgg cgcgcgcgct gacggcgctg ggcttgttcg accgcagcaa
      721 ggcgccgggc cccgacgaca tggcgacgac gagtcagctc aaggatttca acacgaagat
      781 cgcgggaacg ccggcgttct actggctggt gaccgaaggc aacgaccgca agacccaggt
      841 caacgccggc cgggccttcg cacgggcgca gcttgccgcc acggcgcacg gcttgtcgat
      901 gcagccgctg tcgcaggcgc tgcaggaata tccggagcag gtccggcctt acgcggacat
      961 ccatgcgttg ctgcaggcgc cgccgccgcg ctacaccgtg cagatgtggt cgcgcctggg
     1021 ctatgccccg ccggtgggtc ccgcaccgcg gcgaccgttg ggcgacatga tcgtcgcctg
     1081 atccagcagc tccaccagtc gggccagcgc atgctccacg gtgtcgcgcc gcaccgcggc
     1141 gcggtcgccg tcgaagcgct tcatctcgct cgtcagcagt ccgtccacca tgaagccgaa
     1201 ccacacggtg cccacaggtt tttccttgct gccgccggcc gggcccgcga tgccggtcac
     1261 ggcgacgctg acacgggcgg gcgaatggcg tatggcgccg cgagccatgg cgcggaccac
     1321 cgcttcgctc acggccccgt gcgccacgat ggatgcggcg tccacgccca ggagttcgac
     1381 cttggactcg tcggaatagg tgacgaagcc gcgctcgaac cagttgctcg atcccggcag
     1441 ttcggtgcag gcggccgcga tcaggccgcc ggtgcagctc tcggccgtcg ccagcgtcca
     1501 gcccctgtct tgcagcaact gcgccagtct tgtcgtgctc gtcacaagaa cctccatgcg
     1561 gcaatgacca ggagcgtgca gaacgccgcc accaggtcgt cgaagatgat gccgaagccg
     1621 ccgcgccagc cgaagccgtg gaacaggtta tcggcccagg ccacgggtcc gggcttcacg
     1681 gcatcgaaca cccggaacac caggaaggcc cccaactggg cccacaagcc cgccggcatc
     1741 accagccaca gcacgatcca gaaggcgatt acttcgtccc acacgatgct gccgggatcg
     1801 agcacgtgca tgtgggccgc cgtgacggtg caggcccacc agccgatcgc cagcgcggcg
     1861 gcgatcacca ggccgatcgc agcgggcgac aaccagcgtt gcatcagcgc ataggcgagc
     1921 caggcccaga gcgtgccggc ggtgccgggc gccaccggcg acagtccgga gccgaaaccc
     1981 agcgcgatgc aatgcgccgg atgcgacagc atgaagcgca cgctgggccg catgacggct
     2041 gggcgcaacg gcggattgag cggcgtggtc atggcttgcc cgcggcggcg tcgccgctct
     2101 tcgcgaacca ttcggcccag tagtcgatgc aggccttgat cttgggcagg cggtggcggc
     2161 tgggcgcggt cacggcatag accggcaccg ggccggggtc gacgaatttc tccagtaccg
     2221 gcaccagcag tttctgcttg accagcctct cgcccaccag cgtggccagg cggccgatgc
     2281 ccaggccttg caggaccatg gtggccgcca tgttggtgtc gttggtgcgc caatgccctt
     2341 cggcggtgaa aacgaaggcc ttgccgttca cgatgaaagg ccactggttg agcatcgcga
     2401 cgttgctgtt ggcgatgagc cggtgctcgc gcagcgactc cggcagctgc ggcaggccgg
     2461 ccgacgccac ataggagggc gccgcgtaca aggcgcggcc aagcgtgccg agctgccggg
     2521 ccaccgcggt ttccggcaga ttgctgaccg tgcggatggc aatgtcgatg ccgtcgcgaa
     2581 ccatgtccag caggcggtcg ccgacctcca gctccacccg cagccgcggg tgccgcgaat
     2641 tcaggccggg caggctgggc accagctggt actgcgccac gacggtgctg gtggcgacac
     2701 gcaccagccc gctgacctct cgggccttgc tggcgaattc accttcgagc tcgtccaggg
     2761 tgcccacaat cctgcggcaa tagtcgcgga aggtctcgcc ttccggcgtc agcgacaagc
     2821 cgtgtgtgga gcgatggatc agccgggcac cgcaggcttt ttcgatgcgc gagagcgtgc
     2881 gcgaaacctg gctgaccggg acgtcgcgct cgcgggccac ggccgacagc gtgcccagct
     2941 cggcgacgcg agagaacagg tgcacatcgt cgaactggag gtcgcgcatg ggacgaatgg
     3001 taaccatcgc gcggcccttg cctttgcacg gccggcaaga cccatttgcg cgccatgccg
     3061 tttccgctcg cgcctgtttt gcctaaagtg gcttcacacg caaaagcctc tgaaggacag
     3121 caccatgacc acccgcaacc gtaccgactg cagctactac gacatgatcg acgccgccaa
     3181 gctgcgcgcc gaacgactgc gcgacgaagc gatctccgaa ttctggcgcg gcaccggtga
     3241 ggccgcccgg cgcgccgagc gctcggcccg gcgctttgcg agcagcctgg cacgccatgc
     3301 gcgcctgcgg gcccaaagca gagcgtgtcc tgcaccctga acctggcgcg cgggccggca
     3361 ggcgatggcg ccgcccctca ggaatccgtg ccgcagctgc cgtgccgctt gcgaaagtcg
     3421 cgcggccgta ccggagcgtg cccgctccac aaacccagcc cggccctcct ggcttgcgct
     3481 tcctctctgg catagggacc ggggtcgcgg cggaaacggt aggaccatgc gtggccgcgg
     3541 ctgaccatcc agaggcccag gtcctggccc ccctggctga cattgcccag cgcgcgctgg
     3601 tagtcgtcgc gcaggtggct cgcgaccttc acgcgctggt gcaaggcacg ggccgccagg
     3661 gcgtcgcgcg acgcagtccc gtgcacctgg caggattccg gcgcgtcgat accctgcagc
     3721 cggatcttgc gcggtgctcc gcccccggcc ggccggaccc acagcgtgtc gccgtcggtc
     3781 acatgggtca ccacgccttc gaagctgccg gcgcgtgccg aatgggtgag ggcgcacagc
     3841 aaggttgcgg cggcgatgca ctggaaaaac gtcatagccc atccaacgtc gcgcggcaag
//
LOCUS       DASWIY010000367         9438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_991959, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000367 DASWIY010000000
VERSION     DASWIY010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991959"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VGA59_13080"
     CDS             <1..138
                     /locus_tag="VGA59_13080"
                     /inference="COORDINATES: protein motif:HMM:NF022833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEX9720654.1"
                     /translation="GWQADFVSRWMRAFLAAYLVAVPVIYFLAPVARRVTARFAEIPR
                     P"
     gene            135..1109
                     /locus_tag="VGA59_13085"
     CDS             135..1109
                     /locus_tag="VGA59_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9720655.1"
                     /translation="MSAADDHGNASADFNAAFAIGIGLNLAFVAIEAFYGWKVNSLAL
                     LADAGHNLSDLIGLVLAWGGALAGRLRPDARHTYGWKRAGILAAFINALLLLVAMGSL
                     AWEAVHRLQSPEPIEGVTIMVVAGIGIAVNTATALLFMRGRKSDLNIRGAFLHMAADA
                     LVSAGVVVAGGLALWFGWAWLDPVVSLLIAAVIVVGTWNLFKQSLHLLFDGVPEGVDL
                     HAVQALLEALPGVARVHDLHVWAMGTSETALTAHLVMPDDHADDAFLQHATELLHDHF
                     QIEHVTLQVMRVPFTTPCATLAPPAAVHAVGKAPHRKPRLRSVGDEHV"
     gene            1102..1923
                     /locus_tag="VGA59_13090"
     CDS             1102..1923
                     /locus_tag="VGA59_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX9720656.1"
                     /translation="MCELLAVSTSQPTRLTFSLHTLASRGGEAGTTRDGWGVAFYQGD
                     DVALFREPAAAGNSPLVRYLETQGPSTNLAISHIRHATQGAVQLSNTQPFARVLGGRT
                     HVFAHNGDLSGIDRSKALNMGAYRPVGQTDSEHAFCALLERLRPLWENNGPPSLQERM
                     SLLAAFAADLRLFGPANFLYADGDALFAHGHRRIQPASGRIEPPGLWTLQKHRPPVDP
                     SPEHQGGVSISNAARSVVCVASVPLNDEAWRPLAEGELLAVRAGELLATRIAATT"
     gene            1985..2443
                     /locus_tag="VGA59_13095"
     CDS             1985..2443
                     /locus_tag="VGA59_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9720657.1"
                     /translation="MKRAYFLWAPILMIWVSLASAQESPFDGFLTRFDYETRADMKID
                     SKRLITLLAERKAVLVDIRFPEETRAWKMGFGLFIPLNELPGRLNELPKDKIIVTACP
                     HKDRSAIAMTYLRTKGFNARYLTDGLTGLAEKLRGDDAKDFMEDIGMAKP"
     gene            complement(2462..3100)
                     /locus_tag="VGA59_13100"
     CDS             complement(2462..3100)
                     /locus_tag="VGA59_13100"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX9720658.1"
                     /translation="MPEHDPDKASARENLCRFLAACYYEPDAAFAEERLFDSMLAAAS
                     RIDPALAKLAKMLGEGFASQDLETLLVDYTRLFLGPPQPLARPHGAFWLTGETTLMQD
                     ATMAVLDLYQEGGFELDDEFREVPDHVAVELEFLYLLIFKRNEAQRVGSTGDLSAAEQ
                     LGHRFLREHLGAWIAPFTAAVKTGAEAPFYRDLAELTERFVRMEEARLGTAH"
     gene            complement(3170..3949)
                     /locus_tag="VGA59_13105"
     CDS             complement(3170..3949)
                     /locus_tag="VGA59_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9720659.1"
                     /translation="MTRLGMVIDLSKCVGCGACALACKAENNTRTRGSEQSYNWADFL
                     IKTEGKFPNVQHTVMPVMCNHCSDAPCVKACLATPNPNKALYKTPEGITMLDSDKCIG
                     CQRCQEACPYSSEKLGMSSLDGGSYSVISFNFDDEKTQPFWADKASAIPGATSSGAEA
                     AAKVGATVPNMNQWTGTDLKPIRRDGVVEKCTFCSHRTLNGLQPACVEACPAKARIFG
                     DQDDPKSQISQVLKSQKSFRLLEEKGTKPNVHYIGKYSARA"
     gene            complement(3982..6543)
                     /locus_tag="VGA59_13110"
     CDS             complement(3982..6543)
                     /locus_tag="VGA59_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9720660.1"
                     /translation="MRLTRRTFIMASGVSAGWVVTGCAVLAPSVPLHSALVPFKAKPT
                     APAQGAWVASTCQGCTQWCAIEIFTQNGRAVRVRGNQLSKSNHGYVCPRGHLIPQQVY
                     DPDRIKGPMKRTNPVKGRGVDPKFVPISWDEALNTVADKMIELRKAGETHKLVYLRGR
                     YSPTSTNLLYGTLPKVFGTPNYFSHSAICAEAEKMGPGLTQGYFGYRDYDLEKTNCLV
                     VWGADPLASNRQVPNTIHRFGEITKRGTVIAVDPRLSNVAAKAHEWLPIKPGTDGALA
                     GAIAHVLLTEGLWNKEFVGEFKDGRNLFVAGKPVDEAAFAEKETYGLVKWWNLELKDR
                     TPAWAEKETQIPAAQIVRVARAMGKAAPKTAVYMGPGVAMTPRGTYASMAVYALNGLL
                     GSIDVEGGVWQGASGAPLAKFPSVDKYVDDVAKAGSKNKKLDGRGAKDMPAMMGAKPG
                     SGVVTNNVANGMLKDPGAVKVMIASWNNFNFSCTGADRWDKAMAKVPFFVHMVPNASE
                     MTQFADIVLPSTFNSAEGWSIVTNMANGYAYAAIQQPTVKRIWDVKQEENEVMWLLAE
                     KLKAKGFPNLFDYYSKEFKDPETGKSPTTALEFAEISVKMTSAPIWAAKEPVKGDAPI
                     KGWAEFKKKGMFSGARYPLKHGWGGKFKTATKKFEFYSETMKKGLTEHAQKYQTTTDD
                     ILAVSGYLARGDLAFVPHYEAPKRHGSMQDYPFEFVDYKSRLNREGRSQNLPWYQEFK
                     KVDPGDVSWGDVVKMNPADGARLGLKTGDTVKITSQAGAITTQLRLWEGVAPGTVAKC
                     YGQGHWAYGRVAAKDYAKAIPNGGNNNEILVDDYDRLSGSTARNGGFTGVRIQKA"
     gene            7060..7914
                     /gene="phnD"
                     /locus_tag="VGA59_13115"
     CDS             7060..7914
                     /gene="phnD"
                     /locus_tag="VGA59_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9720661.1"
                     /translation="MSKLLKLALLWLWLLASSVQAQTANEPEKIRFAITPAVLNDQHA
                     FLAEWRSYLEDRLKRPVEFVQRDRYRDAMDLLQQQKVDFAWICDYPYVVLKNEVRLLA
                     VALNQGRPTYRASLIVPARDNRTRSISDLKGAVFAYADPYSNTGYLSPRYEIKQGGED
                     PSAFFKRTFFTWSHRKAIEAVAAGLAQGASVDSYVWDVLNRVRPDITAKTRVVWSSKE
                     YGFPPIVAHRNVSNPDFVQMQTVLMNMKNDPQGKALLGRLYLDGFIAGSPALYDGVAE
                     MMRMFGEL"
     gene            7914..9383
                     /locus_tag="VGA59_13120"
     CDS             7914..9383
                     /locus_tag="VGA59_13120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9720662.1"
                     /translation="MRLFDLSLRFKMPLWGGGLILATAIALSGSFAVQAWDNLNEDIL
                     RNAQELGRITAHSLFPVMLHDDMWKAFEIVSSPYAANPEAAATETLTVLDTANRVYVS
                     SRPEEQPVLGELASLGIDYDLLNGELPLAADAGIFVRDARDAQHIYVAIPIANGGVRL
                     GTLLVTYNKSLHRERFLLLLKRTAWITLLALAVLLPITAYWGHRMMQPMRLVTGRISR
                     IGIGELEPLDPFFYPYRDEMGMLFLAYEEMVSKLREKAEFEKEVVKSERMAAVGRLSA
                     SIAHEINNPLGGMLNAISTLRRHGPPDPLTQRTVSLLERGLSQIRETVAALLIQTKVK
                     NRPLTPNDFEDIRILVTPAANKQDTRIGWSVALPAAVSLPATLLRQVLINLMLNAIAA
                     AGQGGRLEVCAQIGGNQFVLMVENDGKALLPEQLNRLFEPFTRFSEQGHGLGLWICYT
                     IVNQLGGTIGAESNLGLTRFTVIIPLDPAHDASAQDLPD"
BASE COUNT         1587 a         2958 c         3011 g         1882 t
ORIGIN      
        1 ggctggcagg ccgatttcgt cagccgctgg atgcgcgctt tcctggcggc ctatctggtg
       61 gccgtgccgg tgatctactt tctggcgccg gtggcgcgac gggtgacggc acgcttcgcg
      121 gagattcccc gaccatgagt gcagcagacg atcacggtaa tgcatcggcc gacttcaacg
      181 ccgccttcgc catcggcatc ggcctgaacc tggcattcgt cgccatcgaa gccttctacg
      241 gctggaaagt caactcgctg gccctgctgg cggacgccgg gcacaacctg agcgacttga
      301 tcggcctggt gctggcatgg ggcggggcgc tggccggccg gctgcggccc gacgcgcgcc
      361 acacctacgg atggaaacgc gccggcatcc tggccgcctt catcaacgcg ctgctgctgc
      421 tggtggcgat gggttcgctg gcatgggaag cggtgcaccg gctgcagtcg cctgagccca
      481 tcgaaggcgt gacgatcatg gtggtggccg gcatcggcat cgccgtcaac acggcaaccg
      541 cgctgctgtt catgcgcggc cgaaagagcg atctcaacat tcggggcgcc ttcctgcaca
      601 tggcggccga cgcgttggtc tccgccggcg tggtggtggc aggcgggctg gccctgtggt
      661 tcggttgggc ctggctcgac ccggtggtga gcctgttgat cgccgctgtc atcgtggttg
      721 gcacctggaa cctcttcaag cagtcattgc acctgctgtt cgacggcgtt cccgaaggcg
      781 tggacctgca tgcggtgcag gccctcctcg aagcgctgcc gggcgtggcg cgggtgcatg
      841 acctgcatgt ctgggccatg ggcacgtcgg agaccgcgct gacagcgcac ctggtgatgc
      901 ccgatgacca cgcggacgat gcgtttctgc agcatgccac cgagctgctg catgaccact
      961 tccagatcga gcatgtcacg ctgcaggtga tgcgggtgcc gttcaccacg ccttgcgcca
     1021 ccttggcgcc gccggcggcg gtccacgccg tggggaaggc gccgcatcgc aagccgcgcc
     1081 tgcggagcgt tggagacgaa catgtgtgaa ctcttggcgg tatcgacctc acagccgacc
     1141 cgcctgacct tttcgctgca cacgctggcg tcgcgtggcg gcgaggccgg caccacacgc
     1201 gacggctggg gggtggcgtt ttatcagggc gacgatgtcg cgctttttcg cgagccggca
     1261 gccgccggta acagcccgct ggtccgctat ctcgaaaccc aggggccgag cacgaacctg
     1321 gcgatctcgc acattcggca tgccacacag ggggcggtac agctgtcgaa cacccagccc
     1381 tttgcacgcg tgcttggcgg ccgcacgcat gtgtttgcgc acaacggtga cctgtccggc
     1441 atcgatcgga gcaaagcgct gaacatgggc gcttaccgtc cggttgggca gaccgattcc
     1501 gagcatgctt tctgcgcgtt gctggagcga cttcggccgc tctgggagaa caacggtcca
     1561 ccgtccttgc aggagcgcat gtcgctgctg gcagctttcg cagcggacct gcgtctgttc
     1621 ggaccagcca actttcttta cgccgacggc gacgcgctgt tcgcacatgg acatcggcgc
     1681 atccagcccg ccagcggccg gatcgagccg cccggactgt ggacgctgca aaagcatcgc
     1741 ccacccgtag acccgtcgcc cgagcaccag ggcggggtat cgatcagcaa tgcagcgcgg
     1801 tccgtcgtgt gcgtcgccag cgtgccgctc aacgacgagg cctggcggcc gctggccgaa
     1861 ggcgagttat tggcggtgag ggcgggtgaa ctcctcgcca cccgcatcgc agccacgacc
     1921 tgactccggc acgccgatcg aaacggacat tcagccagct atcgaacctt gcaggaaaca
     1981 tccgatgaaa cgagcatact ttttgtgggc accaatcttg atgatctggg tctccctggc
     2041 atcggctcag gagtcgccct tcgacggctt tctcaccaga ttcgattatg aaacccgtgc
     2101 cgacatgaag atcgacagca agaggctgat caccttgctg gcggaaagaa aagcggtgct
     2161 ggtggatatc cgtttcccgg aagagacaag agcatggaag atgggattcg gattgttcat
     2221 tccgttgaac gagctacccg ggcgcttgaa tgagctgccc aaggacaaga tcattgtcac
     2281 cgcctgcccc cataaggacc ggtcggccat cgccatgact tacctgcgca cgaaagggtt
     2341 caatgcccgc tacctgacag acggtctgac gggattggcg gaaaagctgc ggggcgacga
     2401 cgccaaggat ttcatggagg acatcggaat ggcaaagccg tgacgtcgtc ctccatgtgg
     2461 ctcagtgggc cgtgccgagt ctcgcttctt ccatgcggac aaaacgctct gtcaactcgg
     2521 ccaggtcgcg gtagaagggg gcctcggcgc ccgtcttgac agcggcagtg aagggcgcaa
     2581 tccaggcgcc caggtgctcg cgaaggaagc gatgcccaag ctgttcggca gcgctcaggt
     2641 cgccggtgct gccgacgcgc tgcgcctcgt tgcgcttgaa gatcagcaag tacaggaact
     2701 ccagttccac ggccacgtgg tccggtactt cgcggaattc atcgtcgagc tcgaaacctc
     2761 cctcctggta caggtcgagc accgccatgg tggcgtcttg catcagcgtt gtctcaccgg
     2821 tgagccagaa cgcgccgtgc ggccgggcga gcggctgggg cggccccagg aaaagccttg
     2881 tgtagtccac gagcagggtt tcgaggtcct gcgaagcgaa cccttcgccg agcattttcg
     2941 cgagcttggc cagggccgga tcgatccggc tcgcggccgc gagcatggaa tcgaacaggc
     3001 gttcttccgc gaacgcggca tcgggttcgt agtagcaagc cgcgagaaag cggcacaggt
     3061 tctctcgtgc gctggccttg tcggggtcgt gttcgggcat cgtcactcct gaaatgaaaa
     3121 ggggccggac aacccggccc cagagttgct ggcgagcggc ctcgagtctt caggcgcgcg
     3181 cgctgtactt gccaatatag tgcacgttgg gcttggtgcc tttttcttcc agcagccgga
     3241 acgacttctg gctcttcagc acctgcgaga tctggctctt cgggtcgtcc tggtcgccga
     3301 aaatgcgcgc cttggccggg catgcttcca cgcacgccgg ttgcaggcca ttgagcgtgc
     3361 ggtgggagca gaacgtgcat ttctcgacga cgccgtccct gcggatcggc ttcaggtcgg
     3421 tgccggtcca ctggttcatg ttcggcaccg tcgcgccaac cttggccgcc gcttccgcgc
     3481 cgctggacgt cgcgccaggg atggccgagg ccttatcggc ccagaacggc tgggtcttct
     3541 cgtcatcgaa attgaagctg atgacgctgt agctgccgcc gtccaggctg gacatgccga
     3601 gcttctcgct gctgtagggg caggcttcct ggcacctctg gcacccgatg catttgtcgg
     3661 aatcgagcat ggtgatgcct tcgggcgtct tgtacagcgc cttgttcggg ttgggcgtcg
     3721 ccaggcaggc tttcacgcag ggcgcgtcgc tgcagtggtt gcacatcacc ggcatcacgg
     3781 tgtgctgcac gttggggaac ttgccttccg tcttgatcag gaagtcggcc cagttgtagc
     3841 tctgctcgga gccgcgggtg cgggtgttgt tttcggcttt gcaggccagg gcacaggccc
     3901 cgcaaccgac gcatttcgag aggtcgatga ccattcccag tcttgtcatg atttactcca
     3961 atgtccttat ggtcggtgtg ttcaggcctt ctggatccgc acgccggtga agccgccgtt
     4021 gcgcgccgtg gagccgctca atcgatcgta gtcatcgacc aggatctcgt tgttgttgcc
     4081 gccgttgggg atcgccttgg cgtagtcctt ggccgccacg cggccgtagg cccagtgacc
     4141 ctggccgtag cacttggcca ccgtgccggg cgccacgcct tcccacagcc tgagctgcgt
     4201 ggtgatggca ccggcttgtg aggtgatctt caccgtgtcg ccggtcttca ggcccagccg
     4261 ggcgccgtcg gccgggttca tcttgaccac gtcgccccag gacacgtcgc ccggatcgac
     4321 cttcttgaat tcctggtacc agggcaggtt ctgcgagcgg ccctcgcggt tcagccgcga
     4381 tttgtagtcc acgaattcga acgggtagtc ctgcatggag ccgtgacgct tgggcgcctc
     4441 gtagtggggc acgaaggcca ggtcgccgcg cgccagatag ccgctcacgg ccaggatgtc
     4501 gtcggtggtc gtctggtact tctgcgcgtg ctccgtgagg cccttcttca tggtctcgct
     4561 gtagaactcg aacttcttgg tggccgtctt gaacttgccg ccccagccat gcttgagcgg
     4621 gtagcgcgcg ccgctgaaca tgcccttctt cttgaattcc gcccaaccct tgattggcgc
     4681 gtcgcccttc acgggctcct tggcggccca gatcggggcg ctggtcatct tgacggatat
     4741 ctccgcgaac tccagggcgg tggtcggcga cttgccggtc tccggatcct tgaattcctt
     4801 ggagtagtag tcgaacaggt tcgggaagcc cttggccttg agcttttcgg ccagcagcca
     4861 catcacttcg ttttcttcct gcttgacgtc ccagatccgc ttgacggtag gttgctggat
     4921 cgccgcgtag gcgtaaccgt tggccatgtt ggtgacgatc gaccagcctt cggccgagtt
     4981 gaaggtcgag ggaagcacga tgtcggcgaa ctgcgtcatc tcggaggcgt tgggcaccat
     5041 atgcacgaag aacggcacct tggccatcgc cttgtcccag cggtccgcac cggtgcagga
     5101 gaagttgaag ttgttccagg aggcgatcat caccttcacg gcgcccgggt ccttgagcat
     5161 gccgttagcg acgttgttgg tcaccacgcc gctaccgggc ttggcgccca tcatcgcggg
     5221 catgtccttg gcgccgcggc cgtcgagctt cttgttcttg ctgccggcct tggcgacatc
     5281 atcgacatat ttgtccacac tcgggaactt ggccagcggc gcgccgctgg cgccttgcca
     5341 cacgccacct tccacatcga tcgagccgag caggccgttg agcgcgtaca cggccatcga
     5401 ggcataggtg ccgcgcgggg tcatcgccac gcccgggccc atatacacgg cggtcttggg
     5461 cgccgccttg cccatggcgc gggccacgcg cacgatctgc gcggcgggga tctgcgtttc
     5521 cttctcggcc caggccggcg tgcggtcctt gagctccagg ttccaccact tcaccaggcc
     5581 gtaggtctcc ttctccgcga aagcagcctc gtcaacgggt ttgcccgcca cgaagaggtt
     5641 cctgccgtcc ttgaattcgc cgacgaactc cttgttccac aggccttcgg tcagcagcac
     5701 atgggcgatc gcgccggcca gcgccccgtc cgtgccgggc ttgatcggga gccactcgtg
     5761 cgccttggcg gccacgttgg acagccgcgg gtccaccgcg atcaccgtgc cgcgcttcgt
     5821 gatctcgccg aaacggtgga tcgtgttggg cacctggcgg ttggaggcca gcgggtccgc
     5881 gccccacacc accaggcagt tggtcttttc caggtcgtag tcgcggtagc cgaagtagcc
     5941 ctgcgtcagt ccggggccca tcttctcggc ttcagcgcag atggcgctgt gggagaagta
     6001 gttgggggtg ccgaacacct tgggcagggt cccgtagagc aggttggtgg aggtgggcga
     6061 gtaccggccg cgcaggtaca ccagcttgtg cgtctcgccg gccttgcgca gctcgatcat
     6121 cttgtcggcg accgtgttca gcgcctcatc ccagctgatg gggacgaact tgggatcgac
     6181 gccgcggccc ttgaccgggt tggtgcgctt catcgggccc ttgatgcggt ccgggtcgta
     6241 cacctgctgc gggatcaggt ggccgcgcgg gcagacatag ccgtggttcg acttgctcag
     6301 ctggttgccg cgcacacgca cggcgcggcc gttctgcgtg aagatctcga tggcgcacca
     6361 ttgggtgcag ccctggcagg tgctggcaac ccaagcccct tgggccgggg cggtcggttt
     6421 ggccttgaag ggcacgagcg cgctgtgcag cggcacgctg ggcgccagga ccgcgcagcc
     6481 ggtgactacc cagcccgcgg acacacccga cgccatgatg aaggttcgtc tagtcagcct
     6541 catgtttgct cctgatggga aatttgtggc ccatgacgac tcagagcctc ttgatcctgt
     6601 gggcgcaagg ttcgtgccaa cgaccgcggg aatgcagaaa gtggggtcga atgcggcaac
     6661 acgggcagat cagtcgcggc gcctccagcg aagagactgg tggattgcga gcagattgct
     6721 gatggccttt tgcgggcgtc atcgagccat ccccgcgcat cggcgcgccg ttaccagccg
     6781 gtaacggact cacgcacaac ggacaatgag cgtccgcact tggcaggcgg aaacttgcgc
     6841 cggccaagca cttatcaggg tcaacgttga caatcgctct tgacactgca taagccgtgc
     6901 cggaatattt tgcttttcgc cagatgtggc gttcatgacc acgcggccct ggcgcgcggg
     6961 tctgcgctgc ggcgcgctcg gaaacgacgg cgttaccatt tggtgacccg aaagtgctgt
     7021 gggttgaacc gggttacctt ccggtagcag gaaaacagga tgtcgaagtt gctcaaactc
     7081 gctctattgt ggctctggct gctcgcctcg agcgttcagg cgcagacggc caatgagccg
     7141 gaaaagatcc gtttcgccat cacacccgcc gttcttaacg accagcacgc cttcctggcc
     7201 gaatggcggt cctatctgga ggatcgtctg aaacggcctg ttgagttcgt gcagcgcgat
     7261 cgctaccgcg acgcgatgga tctgctgcag cagcagaagg tcgacttcgc ctggatttgc
     7321 gactacccgt atgtggtcct caagaatgag gtgcggctgt tggcggtggc gctcaatcag
     7381 ggccgaccca cgtaccgggc ctccctgatc gttcccgcac gcgacaaccg tacgcgcagc
     7441 atctcggacc tcaaaggcgc ggttttcgcg tatgcagacc cttactcgaa caccggctac
     7501 ctctcccccc gctatgaaat caagcaaggc ggcgaggacc cgagcgcttt tttcaagcgc
     7561 acgttcttta cctggtccca tcgcaaggcg atcgaggccg tcgccgcggg cctggcacag
     7621 ggcgcatcgg tggatagtta cgtctgggac gtcctgaaca gggtgcgacc ggacattacc
     7681 gccaagacgc gtgtcgtatg gtcgtcgaaa gaatacggct ttccgccgat cgttgcacac
     7741 cgcaatgttt ccaacccgga ttttgttcag atgcaaaccg tcctgatgaa catgaagaac
     7801 gacccgcagg gcaaggccct gctaggccgg ctctaccttg atggtttcat tgcggggtcg
     7861 cccgcgcttt acgacggcgt agccgagatg atgaggatgt tcggtgaact ttgatgcgcc
     7921 tgttcgatct cagtttgcgc ttcaagatgc ctctttgggg tggtggcctg atcctggcca
     7981 ccgcgatcgc cttgtccggc tcgttcgctg tgcaggcatg ggacaacctg aacgaggata
     8041 ttctcaggaa cgcgcaggaa ttgggacgca tcacggcgca ctcgctcttc ccggtaatgt
     8101 tgcacgacga catgtggaaa gctttcgaaa tcgtttcctc gccctatgcc gcaaatccgg
     8161 aagcagctgc caccgagact ctcacggtcc tggacaccgc caacagagtc tatgtttcgt
     8221 cgcgccccga ggagcagccg gtcctcggtg aactggcatc gctgggcatc gactacgacc
     8281 tcctgaacgg cgagctgccg ctggcggccg atgccggcat ctttgtgcgc gatgctcgcg
     8341 atgcccagca tatttacgtt gccatcccga tcgccaacgg cggcgtgcgg ctcggcacgc
     8401 tgctcgtcac ctataacaag tcgcttcaca gggagcgatt cctgctgctt ttgaaacgca
     8461 ctgcgtggat cacgctgctc gcactcgcgg tgctgcttcc catcaccgcc tattgggggc
     8521 atcgcatgat gcaaccgatg cggctggtga ccgggcgcat cagccgcatc ggcattggtg
     8581 agctcgaacc actcgatccg ttcttctatc cttatcgcga cgagatgggc atgctttttc
     8641 ttgcctatga ggaaatggtg tccaagttgc gcgaaaaggc cgagtttgaa aaggaagtgg
     8701 tcaagagcga gcgcatggcg gccgtgggcc ggctgagcgc cagcatcgcg cacgagatca
     8761 acaatccgct gggcggcatg ctgaacgcga tcagcacgct caggcgccac ggccccccgg
     8821 acccgctgac gcaaaggacc gtttcgctgc tggaacgcgg gctttcgcaa atccgcgaga
     8881 cggtcgccgc gctattgatc caaaccaagg tcaagaaccg gccgctgacc ccgaacgact
     8941 tcgaagacat ccgcatcctg gtgacgccgg cggcgaacaa gcaggacacc cggatcgggt
     9001 ggtcggtcgc cctgcccgct gccgtttccc tgcccgcgac gctgctgcgc caggtgctga
     9061 tcaacctgat gctcaacgcc attgccgccg ccggacaggg tggcaggctt gaagtctgcg
     9121 cgcagattgg cgggaatcaa ttcgtgctga tggtcgagaa cgacgggaag gccctgctgc
     9181 cggagcaact gaaccgtctg ttcgagcctt ttacgagatt ttccgagcag ggccatggtc
     9241 tcggcctgtg gatttgctac accatcgtca atcaactggg cggtaccata ggcgccgaat
     9301 ccaatctcgg gctgacccgc tttaccgtaa tcattccttt ggaccctgct catgacgcaa
     9361 gcgcccagga tctgcctgat tgaagacgat gagatcatgg gcgaatcgct ggccgaacga
     9421 ttcggcctcg agggcctc
//
LOCUS       DASWIY010000368        11693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2483981, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000368 DASWIY010000000
VERSION     DASWIY010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11693
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2483981"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1010
                     /locus_tag="VGA59_13125"
     CDS             69..1010
                     /locus_tag="VGA59_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX9720663.1"
                     /translation="MKNGGDRHFDVVALGEGMLEFNQTAAGQPNYLQGFGGDTSNAVI
                     AAARAGANTAYLTRVGGDAFGRSLRELWQSEGVDSRAIETGEEAPTGIYFVTHGPAGH
                     EFSYRRAGSAASRMTPGWLLDGPASPVIRDAKILHLSGISLAISASARETSLAAMKLA
                     RGSGTRVSFDPNLRLKLWSLEQAREGIATAMALSEIFLPSLEDVTALTGIHEVDAVID
                     WGHAAGAPVVVLKLGAEGAVASDGQRRERIASHAVPTVDATGAGDCFCGNLLARVAAG
                     DTVFEAARYANAAAAVAVQGFGAVAPLPRSAQVRALL"
     gene            1007..1903
                     /locus_tag="VGA59_13130"
     CDS             1007..1903
                     /locus_tag="VGA59_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEX9720664.1"
                     /translation="MKQLIALDWGTSSLRAAKLDAQGRVVEERSLPRGILTVAPGEFP
                     AVFEAACAGWLREGDSAALLSGMVGSRQGWLDAPYCPCPAGFADVAAQLAWVEPARIA
                     IVPGLSCERQGMPDVMRGEETQVFGAMQLLGIDRGLFVLPGTHSKWVRVAGQRIESFS
                     TYMTGEFYGLLRRHSILARTLPEADGELDTQAFLRGMKHAMRSGNLLHAAFSARTLSL
                     FDRLPAAALPSYLSGLVIGEELRSQQLAALSGPIVLVGAPSLTQRYELALLALGVPVR
                     SAGSEATWRGLWAIAQTLDAAP"
     gene            1900..2538
                     /locus_tag="VGA59_13135"
     CDS             1900..2538
                     /locus_tag="VGA59_13135"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEX9720665.1"
                     /translation="MNAAARFAAALAACPLVAILRGLRPQEALPVGQALAGNDFKLIE
                     VPLNSPQPLLSIQAIAAAFPHALVGAGTVLEPRQVRQVHAAGGRLIVAPNFNPAVVRE
                     AARLDMICLPGVMTATEAFAALAAGATGLKLFPAEMIPPAAVKALRAVLPADALLLPV
                     GGIGTADMASYRAAGADGFGIGSALYKPGMAPAEVKSKAVDFIAAWTGTLRA"
     gene            2621..3163
                     /locus_tag="VGA59_13140"
     CDS             2621..3163
                     /locus_tag="VGA59_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720666.1"
                     /translation="MNEFFLWGLAVVALALAITAGARVLGRRRHDRLRARGYELIFSL
                     KSYSAWVDSWCDEPLMSRIPDELALPEPLARAREIRDTAFPGLSQHMVRLLQSHSRLI
                     EHLWRHKLLRLSQATGWQPAWQDPHYQQIRGAQEDLIQEMIGLTQEMIGDRAQEWRRT
                     GTDFAFSSGMSTSLGPTAGA"
     gene            complement(3192..3779)
                     /locus_tag="VGA59_13145"
     CDS             complement(3192..3779)
                     /locus_tag="VGA59_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HEX9720667.1"
                     /translation="MRTLQFAIAVLAASLALSACKEGPDASPAKGSVAATPVSTAAIE
                     ADARGFTVGSPMSARTVYVFFDPQCPHCAALWKSAKPLKAQAKFIWIPVALLNKTSEA
                     QGAALLAAKDPVALMDEHEASISAKGGGISAMGAPDPERATVVKNTALMNRFGFASVP
                     SIVAMHAQTGALVTREGGLPTPELAALLGLQVPAQ"
     gene            complement(3848..6070)
                     /locus_tag="VGA59_13150"
     CDS             complement(3848..6070)
                     /locus_tag="VGA59_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720668.1"
                     /translation="MSELRLWALRAWRAGRALWVRWGRRWSPQPGWWRLPVRFGAVLV
                     LGTWMAGLVVAAVQLDAWDDELVRTLLQIRADAVFRDRMADGREPVPREWYRSKTLAL
                     LAASEKLHDDTKWTLFVPGSWRPLDGLRQRVAARIELAFSDIVVETVRRELYFRASQL
                     SGVAQDSHTAEPLATGDCAPPPVAVGLAGWGKAASAPQELPEYAAVRKQLAAIEQLDR
                     AAQALLALQSPGGDAENLRLLARYTLGVELPGQLSRSAAFFRTGLKPEDVTYTARGLP
                     RMQRALRCSLAKAMFALDVRLFERNDLLATEAFLAQRASRLFAPRARPGPFSDMVQGY
                     REVIAALNEQEALLARSEYAWLHHRGAGVGPHDGLLARINRVSLLGPEAVEQIRRQSG
                     AAAQRFRRQFGMVFGADNDPALVWDETRGRLVLSPQRLALRDGLTALLQEPFMVAPAD
                     RVFPEAAHGPLTWDSQRLEQALGLADAHRRFVDDNLPEFPPAVQQPIARFVEAHLAQL
                     VQDATIEAIRPGAPGTTLSDFSSYREQREQLGKVQALLVDMGARSKAYKLRSVLSQDL
                     LGRLALAEAAMWRSPIFSDRARHFGWWQGEGSPVLQAFGVADRLTLRYLLSQQLRHLD
                     EIAREAGSYLAYVDPSIAASGEVKRWQGMVAELKRYRAGTADSSLLALERYLLWLGPE
                     LNRSNCVERLSFAAPANGGSDEFARRHVQFHNALLGRCVELRSPGGVIEPPRPVTPFG
                     "
     gene            complement(6477..7709)
                     /locus_tag="VGA59_13155"
     CDS             complement(6477..7709)
                     /locus_tag="VGA59_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9720669.1"
                     /translation="MNSAANGPGRHVALWLILLAAAGTFALTMGTRQTMGLFLSPLNT
                     STGLGLGSISLAFAFGQLWWGLTQPFAGAMADKVGAGRVLFAGAVLVALGTFITPYMT
                     TTAGLILAIGVLSAGGAGMAGPAVLMAATTRLISPARRGLATGIVNAGGSFGQFVMAP
                     IAAALMVGVGWASALQVMGLLVLLCLPAAFLLKGNSLQSAPAGHKVMGTREAVRAALR
                     NPSYLMLGAGFFVCGFHVAFLATHLPGVIASCGLPTQWAGWSLAIIGLFNIVGSVAMG
                     WAVGRWRMKSLLSLVYAARGLAVIAFLLAPKTGPVILLFAAVMGLTFLSTVPPTVGLV
                     AKFFGTSNMATLFGVVMLGHQVGGFLGAWLGGTVFDATGAYDWVWYIDVMLAVGAALI
                     HLPIREAPPRAAGKTAAA"
     gene            complement(7702..8157)
                     /locus_tag="VGA59_13160"
     CDS             complement(7702..8157)
                     /locus_tag="VGA59_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX9720670.1"
                     /translation="MDTAVKPQGCTNLKLRQLMRRVAQHYDAEVGKTGLKGTQYSLLS
                     YVVKLGPIRPGDLAREMKIGASTLTRNLRPLIDAGWLELGPGADHRSRLVSVTQAGRE
                     KRQEAQRRWRVAQEGINRMLGAHRVVELHALIDECLDLLSPLPHGETDE"
     gene            8341..8880
                     /locus_tag="VGA59_13165"
     CDS             8341..8880
                     /locus_tag="VGA59_13165"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9720671.1"
                     /translation="MAQMNHLETWLAQEQEVMARLDAGVGAGVASPQQLAGQSGLEIM
                     RSMLRGELPYPPIAKTLDFALLEVEEGRALFQGTPGPAHLNPMGTIHGGWYATLLDSA
                     MGCAVHTMMPAGRGYTTAELGVNLVRAINGKAQRVRAEGKVIHCGRQLATAEARLYGP
                     DGTLYAHATTTCLVFEVRA"
     gene            complement(8893..9024)
                     /locus_tag="VGA59_13170"
     CDS             complement(8893..9024)
                     /locus_tag="VGA59_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720672.1"
                     /translation="MLSGREDPHLPAQAIEAGANRFFLKPADWCLLASAASKMLPPT"
     gene            complement(9039..10424)
                     /locus_tag="VGA59_13175"
     CDS             complement(9039..10424)
                     /locus_tag="VGA59_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX9720673.1"
                     /translation="MKKMRTRIALVMCAASLAGCSMMPAYERPAAPVPGEWPYAAAST
                     GNPASELKWEDFFADARLKLLIAIALRNNRDLRVAVLNIEQARAQYDIRRADRFPSVG
                     VTAGGSRTPKSGGGTTSAYTAGLAFTAWEIDLFGRVASLGEAALAQYLATEEGRKAAQ
                     TSLISAVANNWLALLADEELIALTRQTLATREESLRLTKLRFDHGVASELDFRQAQSL
                     VEGARVTLSQLIRQRAIDLNALALLLGQAVPSDFQPAADTASVALPDPPAGMPSDVLV
                     RRPDVRQAEQQLIAANANIGAARAAFFPRITLTAGIGTASSELSGLFKGGSWGWPAAP
                     QLLQPIFDAGRNRAGLAAAHASRDIAVAQYEKAIQSAFREVADALAGRATFGEQLQAQ
                     RNVADAEAVRYRLSELRYNAGVASYLDLLDAQRSLFAARQALVQTRLQQLQNQVQLYK
                     ALGGGWEPRQD"
     gene            complement(10421..>11693)
                     /locus_tag="VGA59_13180"
     CDS             complement(10421..>11693)
                     /locus_tag="VGA59_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9720674.1"
                     /translation="RKGDGQSAQALAGRAFGALSGIRDAIIFPLSPPPISELGTASGI
                     TFRLQDRGGAGPAALIAARNQLIALAGQSKVLTQVRPDGLEDAPQLQIDIDRDKANAL
                     GVPFEAINSTLSTALGSSYINDFPNRGRLQRVVVQADAPARMQPADLLRLTASNNQGK
                     AVPLSAFASTRWISGAMQTVRYNGYPAMRISGSPAPGYSTGAAMAEMERLVAQLPQGF
                     GYEWTGQSREEKLAGAQAMILYGFAILSVFLCLAALYESWSIPLSVILIVPLGVLGVL
                     LATLLRGYANDVYFQVGLITIIGLSAKNAILIIEFAKDLQAQGKSVVESALIAAHLRF
                     RPIVMTSMAFGLGVLPLALASGAGSASQRAIGTGVLGGMLTGTGLAVFFVPIFFVIVR
                     GFFKGSERQRRLYTHDLDKDLPAGAAAGDRI"
BASE COUNT         1965 a         4017 c         3958 g         1753 t
ORIGIN      
        1 ggacaatcat tgatggacat gcggatcaag cctgccccgg actccgatcc ggggtccgca
       61 atgacagcat gaaaaatggc ggcgaccgcc acttcgacgt cgttgccctg ggcgaaggga
      121 tgctggaatt caaccagacc gccgccgggc agccgaacta cctgcagggc ttcggcggcg
      181 acaccagcaa tgccgtgatc gcggctgcgc gcgcgggcgc caacacggct tacctgacgc
      241 gcgtcggcgg cgacgcgttc ggccgctcct tgcgcgagct ctggcaaagc gagggcgtgg
      301 acagccgggc catcgagacg ggcgaggaag cgccgacggg catctatttc gtcacccatg
      361 ggccagcggg gcatgagttc agctaccgcc gcgccggttc cgcggccagc cgaatgacgc
      421 cgggctggct gctggacggg cccgcctcgc cggtcatccg cgacgcgaag atcctccacc
      481 tttccggcat ttcgctggcg atctccgcca gcgcgcgcga aacgtcgttg gccgcgatga
      541 agctggcccg cgggtcggga acgcgggttt cattcgatcc caacctgcgg ctcaagctct
      601 ggagcctgga gcaagcccgt gaaggcattg caacggcgat ggccttgagc gaaatcttcc
      661 tgcccagcct cgaagacgtg accgcgctca cgggaatcca cgaggtcgat gcggtgatcg
      721 actggggcca tgccgccggt gcgccggtcg tggtgctcaa gctgggggcc gaaggcgccg
      781 tggccagcga tggacagcgc cgcgagcgga tagcgagcca cgccgtgccc acggtggacg
      841 ccaccggggc cggggattgc ttctgcggca acctgctcgc gcgcgtggcc gccggcgata
      901 ccgtcttcga agccgcccgc tacgccaacg ccgccgcagc cgttgccgtg caaggtttcg
      961 gtgccgtcgc gccgctgccg cgctccgcgc aggtaagggc cctgctgtga agcagctgat
     1021 cgccctggac tggggaacct cgtcgctgcg cgcggcgaag ctcgatgcgc aaggccgggt
     1081 ggtggaggaa cgctcgctgc cgcgcggcat tctcaccgtg gcgccgggcg agttcccggc
     1141 cgtgttcgaa gccgcatgcg ctggctggct gcgcgagggc gacagcgccg cgctgctcag
     1201 cggaatggtc ggcagccgcc agggctggct cgacgcgccc tactgcccct gccccgccgg
     1261 ctttgccgac gtcgcggcgc agctggcctg ggtggagccc gcgcgcatcg cgatcgtgcc
     1321 cggcctgagc tgcgagaggc aaggcatgcc cgacgtgatg cgcggcgagg aaacccaggt
     1381 gttcggcgcg atgcagctgc tcggaatcga caggggcctg ttcgtgctgc ccggcacgca
     1441 cagcaaatgg gtgcgcgtcg ccggacagcg catcgaatcc ttttcgactt acatgacggg
     1501 cgagttctac ggcttgctgc gccggcactc gatcctggca cgcacgctgc ccgaggccga
     1561 cggcgaactc gacacgcaag cctttctgcg cggcatgaag catgcgatgc gaagcggcaa
     1621 tctcctgcac gccgccttca gcgcccgcac gctgtcgctc ttcgaccgcc tgcccgcggc
     1681 agccctgccg agctacctct cgggcctcgt gatcggcgaa gaactgcgtt cacagcaact
     1741 ggcggccctg tccgggccga tcgtcctggt cggcgctcca tccctcacgc aacgttatga
     1801 actggcgctg ctggcgctcg gcgttcccgt gcgcagcgct ggatctgaag caacctggcg
     1861 cggcctgtgg gccatcgcgc aaacgctgga cgctgcccca tgaacgcagc cgcgcgattc
     1921 gcagccgcgc tcgcggcttg ccccctggtc gcgatcctgc gcggcctgcg gccccaggaa
     1981 gcccttcccg tcgggcaggc cctggccggc aacgacttca agctgatcga agtgccgctg
     2041 aattcaccgc agcccctgct gagcatccag gccattgcag cggcattccc gcacgcgctg
     2101 gtgggggccg gaaccgtcct cgagccccgg caggtccgcc aggtgcacgc ggccggcggc
     2161 cggctgatcg tggcgccgaa cttcaatccc gcggtggttc gcgaagcggc gcggctggac
     2221 atgatctgcc tgcccggcgt catgacggcg accgaggcct tcgccgcgct ggctgccggt
     2281 gccacgggcc tgaagctgtt tccggccgag atgatcccgc ccgccgcggt gaaggcgctg
     2341 cgcgcggtgc tgcccgcgga cgcgttgttg cttcccgtcg ggggcatagg cacagccgac
     2401 atggcaagct accgcgccgc gggcgcggac ggtttcggca tcggctcggc cctgtacaag
     2461 cccggcatgg cgccggccga ggtgaaaagc aaggcagtcg acttcatcgc cgcatggacg
     2521 ggtaccttgc gggcatagga acgccgctcg tcggaacagg accacctgcc atcgcgccgg
     2581 gcacggccgc ggtgccgcgg ggcgcctaga ctgatgtccc atgaacgagt tcttcctttg
     2641 ggggctggcc gtcgtggcgc tggccctggc catcactgcc ggggctcgcg ttctcggccg
     2701 caggcgccac gaccggctgc gggcccgcgg gtatgagctc atcttctcgc tgaagtccta
     2761 ttcggcctgg gtggacagct ggtgcgacga gccattgatg agcaggattc ccgatgaact
     2821 cgccttgccg gagccgctgg cgcgtgcccg cgagatcagg gacactgcgt tccccggcct
     2881 gtcgcagcac atggtgcgcc tgctacagtc gcacagccgc ctgatcgaac acctctggcg
     2941 gcacaaactc ttgcggctga gccaggcgac cggctggcag ccggcctggc aggatccgca
     3001 ttaccagcag atccgcggcg ctcaggaaga cctgatccag gagatgatcg gcctcaccca
     3061 ggagatgatc ggcgaccgcg cgcaggaatg gcggcgcacc ggcaccgact tcgctttctc
     3121 cagcggcatg tccacaagcc tggggcccac ggccggggcc tgaccaccgg ccggctaccg
     3181 cgctcgagca ttcactgggc gggaacctgc aacccaagca gggccgccag ctccggcgtg
     3241 ggcaaaccgc cctcgcgggt caccaaagcg ccggtctgcg catgcatggc gacgatgctg
     3301 ggaaccgagg cgaaaccgaa ccggttcatc agtgccgtgt tcttgaccac cgtcgcgcgt
     3361 tccgggtcgg gggcgcccat ggccgagata ccgcctccct tcgcgctgat cgaagcctca
     3421 tgttcgtcca tgagagccac cggatccttg gcggccagca aggctgcgcc ctgggcctcg
     3481 ctggtcttgt tcaggagggc aaccggaatc cagatgaatt tcgcctgcgc cttcagcggt
     3541 ttggcggact tccatagcgc ggcgcagtgc gggcactgcg gatcgaagaa gacatacacg
     3601 gtgcgcgcgc tcatgggcga gccgacggtg aatccgcgcg cgtcggcctc gatggccgcg
     3661 gtggaaaccg gggtcgcggc gacggagccc ttggcgggcg atgcgtcagg tccctccttg
     3721 caggcggaca aggccaggct ggcggccagg acggcgatgg cgaattgaag tgttctcatg
     3781 cgcgattctc gaagttgggc tactgccgaa gataccaaac tcgctgcggg cccgactcgg
     3841 ggatcgctca tccgaacgga gtgacgggcc gcggcggctc gatgacgccg cccggcgagc
     3901 gcagttcgac gcaccggccg agcagcgcgt tgtggaactg gacatgccgc cgcgcaaact
     3961 catccgaccc cccattggcc ggagcggcga acgagagccg ctcgacgcag ttcgaccggt
     4021 tcaactccgg tcccagccaa agcaggtagc gctccagcgc aagcaggctg ctgtcggctg
     4081 tgcccgcccg gtaccgcttc agctcggcca ccatgccttg ccagcgcttt acctccccac
     4141 tggcggcgat cgacggatcc acataagcaa gatagcttcc agcctcgcgc gcgatctcat
     4201 cgagatggcg cagctgttgc gacagcaggt accgcagcgt cagcctgtcc gcaacgccga
     4261 aggcttgcag gaccggcgat ccctcgccct gccaccagcc gaagtgccgg gcgcgatccg
     4321 aaaagatagg gctgcgccac atcgccgcct cggccagcgc gagacgcccg agcaggtctt
     4381 gcgacagcac ggagcgcagc ttgtacgcct tgctgcgcgc acccatgtcc accagcagcg
     4441 cctggacctt ccccagctgt tcgcgctgct cccgatagga cgaaaaatcc gacagggtcg
     4501 tgccgggggc gccaggcctg atggcttcga tggtcgcatc ctgcaccagc tgcgccaggt
     4561 gcgcttccac gaaccgggca atgggctgtt gcaccgcggg cggaaactcg ggaaggttgt
     4621 cgtcgacgaa gcgccggtgc gcgtctgcca ggccaagagc ctgctcgagg cgctgcgaat
     4681 cccaggtcag aggcccgtgc gcggcctcgg gaaaaacgcg atcggccggc gccaccatga
     4741 acggctcttg cagcaatgcg gtgagtccat cgcgcaaggc caggcgctgc ggcgacagca
     4801 ccagccggcc gcgagtctca tcccagacca gggcaggatc attgtccgcg ccgaacacca
     4861 tcccgaactg gcgcctgaac cgctgggcgg ccgcaccgga ctggcggcgg atctgctcca
     4921 ccgcttccgg tcccagcagg ctgacgcggt tgatgcgcgc gagcaggccg tcgtgcgggc
     4981 cgacgccggc gccgcgatgg tgcagccatg cgtattcgga acgcgccagc agcgcctcct
     5041 gctcattgag cgcggcaatc acttcccgat aaccctggac catgtcgctg aacggccccg
     5101 gcctggcgcg cggtgcgaac agccgcgacg cccgctgggc aaggaaagcc tcggtggcga
     5161 gcaggtcgtt gcgctcgaac aggcgtacat ccagcgcaaa catcgccttg gccaggctgc
     5221 agcgcagggc ccgctgcatg cggggcaaac ccctggctgt gtatgtcaca tcctcgggct
     5281 tcaggccggt gcggaaaaaa gcggcgctgc gcgagagctg ccccggcagt tccacgccca
     5341 gcgtgtagcg cgccagcagg cgcaaattct ccgcatcgcc gccggggctc tgcagggcca
     5401 gcagggcctg cgccgcgcgg tcgagctgtt cgatcgccgc cagctgtttg cgcaccgcgg
     5461 cgtattccgg cagttcctgc ggcgcgctcg cggccttgcc ccatccggcc aggccgaccg
     5521 ccaccggcgg cggcgcgcag tcgccagtgg cgagcggttc agccgtatgg ctgtcctggg
     5581 ccacgccgct caactggctg gcccggaagt agagttcgcg gcggaccgtc tccacgacga
     5641 tatcgctgaa ggcgagctcg atgcgcgcgg ccacccgctg cctcaggccg tcgagcggcc
     5701 gccaggagcc gggcacgaac agcgtccact tcgtgtcgtc atgcagtttt tcgctggccg
     5761 cgagcagcgc cagcgtcttg ctgcgatacc actcgcgggg aaccggctcg cgcccgtcgg
     5821 ccatgcggtc gcgaaaaacc gcgtcggccc ggatctgcag caacgtgcga acgagctcat
     5881 cgtcccacgc atcgagctgg acagcggcca cgaccagccc ggccatccac gtcccgagaa
     5941 ccaggacggc gccgaaacgc accggcaagc gccaccaccc cggctgaggg gaccagcgcc
     6001 ggccccaccg gacccacaac gctcgcccgg cccgccatgc tcgcagggcc caaaggcgca
     6061 actcgctcat atggacgctc ccgcgatcgc ctgttcgccg tcgagcacct gtcgcaccgg
     6121 ccacgaatgc cagagccaaa gaatttcgga caggacgagc agtgcggctg caatcaggac
     6181 atacggccac gccatccgcg ggggccgcaa tcgccgcgac caggccggcc atgaatggct
     6241 ggggtggggg ctgggccgcg ccgctgtttc aaggggcatg ttgcgtctct tcggaggctc
     6301 tcgcaatgcg agtttcgaag aattatcgaa ggcgcggcgg caactgtggg cgctgccggc
     6361 gtgagtgttt cgaagatcaa cgtctgctta ccgggcgcgc cgaaaacacg caaacgcgat
     6421 gagcaccagt cagagccgct gcagagctgc ggcggagccg cccccagggg gcgttcttat
     6481 gcagccgcgg tcttgcccgc tgctcgcggc ggcgcctcgc gaatcggcaa atggatcagc
     6541 gccgcgccca cggccagcat gacgtcgatg taccagaccc agtcgtaggc gccggtcgcg
     6601 tcgaacaccg tgccaccgag ccacgcgccg agaaaaccgc cgacctggtg cccgagcatg
     6661 accacgccga acagcgtcgc catgttgctg gtaccgaaga atttggcgac caggcccacc
     6721 gtcggcggca ccgtggacag gaacgtgagc cccatgacgg cggcgaacag caggatgacg
     6781 ggccccgtct tgggcgcgag caggaatgcg atcaccgcga gaccgcgtgc ggcatagacc
     6841 agggacagca gcgacttcat gcgccagcgg ccgacggccc agcccatcgc gacgctgccg
     6901 acgatgttga acagcccgat gatggccagc gaccagccgg cccattgcgt cggcagtccg
     6961 caggaagcga tcacgccggg cagatgggtg gccagaaagg ccacgtggaa cccgcagacg
     7021 aaaaatcccg cacccagcat cagatagctg ggattgcgca gcgcggcgcg gaccgcctcg
     7081 cgcgtgccca tcaccttgtg tccggctggc gccgactgca gcgagttgcc cttgagcagg
     7141 aaggccgcgg gcaggcacag cagcaccagc aggcccatca cctgcagcgc gctggcccat
     7201 cccaccccga ccatcagcgc ggcggcaatc ggcgccatga cgaactggcc gaatgagccg
     7261 cccgcgttga cgatgccggt ggcaaggccg cgccgggcgg gggaaatcaa tcgcgtggtg
     7321 gccgccatca gaacggcggg accggccatg ccggcaccac cggccgacag caccccaatc
     7381 gcgagaatca agcccgcggt ggtcgtcatg tagggcgtga tgaacgtccc caatgccacc
     7441 agcacagcgc cggcgaacag cacgcgcccc gcgccgacct tgtcggccat ggcgcccgcg
     7501 aacggctggg tcagtcccca ccagagctgg ccgaaggcga acgcaaggct gatgctgccc
     7561 aggcccagcc ccgtcgaggt attcaagggc gagaggaaaa ggcccatggt ctgccgcgtg
     7621 cccatggtca gcgcgaaagt gccggccgcg gccagcagta tcagccaaag cgcgacatgc
     7681 ctgcccgggc cgttcgctgc actattcatc ggtctctcca tgaggcaagg gtgaaagcaa
     7741 gtccaggcat tcgtcgatga gcgcatgcag ctcgaccacg cgatgcgcgc ccagcatccg
     7801 gttgatgcct tcctgcgcga cgcgccagcg gcgttgcgcc tcctgacgtt tttcgcggcc
     7861 ggcctgggtg acactgacaa gccggctgcg gtggtcggcg cccggcccca gctcgagcca
     7921 gccggcgtcg atcaaaggcc gcaggttgcg ggtgagcgta gaggccccga tcttcatctc
     7981 gcgcgccagg tcgccgggac ggatcgggcc gagcttgacg acataggaca gcagcgagta
     8041 ctgggttccc ttcaagcccg tcttgccgac ctcggcgtcg taatgctgcg ccacgcggcg
     8101 catcagctgg cgcagcttca ggttggtaca gccttgcggc ttgacagcgg tgtccatgtc
     8161 gattattgta gctgcaattg ttgtagatgc aagaatattg agacattgcg ggacagacct
     8221 tcagttctgt ccccaacatt tcgagatttg cgggacagac cttcagctct gtcctcaact
     8281 tttttgaaac cttgcgggac agaccttcag ctctgtcccc aactgactag aggaacgacg
     8341 atggcgcaga tgaaccacct tgaaacatgg ctcgcccagg aacaggaagt gatggcgcgg
     8401 ctcgatgccg gcgtcggtgc cggagtcgcc agcccgcaac agctggccgg ccagagcggg
     8461 ctggaaatca tgcgctcgat gttgcgcggt gagttgcctt acccaccgat cgcgaaaacg
     8521 ctggacttcg cgttgctgga agtggaggaa ggccgtgccc ttttccaggg cacgccggga
     8581 ccggcccatc tcaacccgat gggcaccatc catggcggct ggtacgccac gctgctggac
     8641 tcggccatgg gatgcgccgt gcatacgatg atgccggcag gacgcggcta caccaccgcc
     8701 gagctgggcg tgaacctggt ccgggccatc aatggcaagg cccaacgcgt tcgcgccgag
     8761 ggcaaggtca tccattgcgg ccggcagctg gccacggcgg aggcgcgtct ttatgggccg
     8821 gacggaacgc tgtatgccca cgcgaccacc acctgcctgg tgttcgaggt gcgggcctga
     8881 agcaatctgt gttcaggtcg gcggcagcat cttcgatgcg gcgctggcca gcaggcacca
     8941 atcggccggt ttgagaaaga accggttggc tccggcctcg atcgcctgtg cgggcaagtg
     9001 cgggtcttcg cggcccgaca gcatcagtat cggaaaagct agtcctggcg cggctcccag
     9061 cccccgccca gcgctttgta aagctgcacc tggttctgca actgttgcaa tcgcgtctgc
     9121 accagggcct gccgggcggc gaacaacgag cgttgggcgt ccagcaggtc gaggtagctg
     9181 gccacgccgg cgttgtagcg cagctccgat aggcgatagc gcacggcctc tgcatcggcc
     9241 acgttgcgct gcgcctgaag ctgctcgccg aaggtggcgc gcccggccag cgcatcggcc
     9301 acttcgcgga aagcggactg gatggctttc tcatactggg cgacggcgat atcgcgcgac
     9361 gcatgggccg cggccaaccc ggcgcggttg cggccggcgt cgaagatggg ttggagcaat
     9421 tgcggcgccg cgggccagcc ccacgatccg cccttgaaca gccccgagag ttcgctgctc
     9481 gcggtgccga tgcccgcggt gagcgtgatg cgcgggaaga acgcggcacg cgcggcgccg
     9541 atgttggcgt tggccgcgat cagttgctgc tccgcctggc gcacatcggg ccgccgcacc
     9601 agcacgtcgg acggcatgcc cgcgggcggg tccggcagag caacggaggc cgtgtcggct
     9661 gccggttgaa aatccgacgg cacagcctgg cccagcaaga gcgccaatgc gttcaggtcg
     9721 atcgcacgct ggcggatcag ttgcgacagc gtgacgcgag caccctcaac gagcgactgc
     9781 gcctgccgga agtccagctc ggaggccact ccatggtcga agcgcagctt ggtcagccgc
     9841 agcgactctt cgcgcgtcgc cagcgtctgg cgtgtcaacg cgatcagttc ctcgtcagcc
     9901 agcagggcca gccagttgtt cgcgacggcg gaaatcaggc tggtttgcgc cgcttttcgc
     9961 ccttcctcgg tcgcgaggta ttgggccagc gccgcctcgc ccaggctcgc cacacggccg
    10021 aacaaatcga tctcccaggc ggtgaacgcc aggccggccg tgtaggcact ggtcgtgccg
    10081 ccgccgctct tcggggtgcg cgatccgccg gccgtgacgc cgacactggg aaaccggtcg
    10141 gcccggcgga tgtcgtactg ggcgcgggcc tgctcgatgt tcagcaccgc cacgcgaagg
    10201 tcgcggttgt tgcgcagggc gatcgcgatc agcagcttca ggcgcgcgtc ggcaaagaaa
    10261 tcttcccact tcagctcgga cgcggggttg ccggtggagg ccgcggcgta aggccactcg
    10321 ccgggcaccg gggccgcggg tcgctcatag gcgggcatca tcgagcagcc cgccagcgag
    10381 gcggcgcaca tgaccaaagc gatacgcgtg cgcatcttct tcatatccgg tcccccgcgg
    10441 cggcgccggc cggaaggtcc ttgtccaggt cgtgggtgta aaggcgccgc tggcgttcgc
    10501 tgcccttgaa gaaaccgcgc acgatcacga agaagatcgg cacgaagaaa acggcgaggc
    10561 cggtgccggt gagcatgccg cccagcacac cggtgccgat cgcacgctgg ctggccgagc
    10621 ccgcgcctga cgccagcgcc agcggcaata cccccagccc gaacgccatc gaggtcatca
    10681 cgatcgggcg gaagcgcaag tgcgcggcaa tgagcgccga ttcgaccacg ctcttgccct
    10741 gggcctgcag gtccttggcg aactcgatga tcaggatcgc gttcttggcc gagaggccga
    10801 tgatggtgat caggcccacc tggaaataga cgtcgttggc atagccgcgc agcagcgtgg
    10861 ccagcagcac gcccagcacg cccagcggca cgatcaggat caccgacagc gggatcgacc
    10921 agctttcgta cagcgcggcc aggcacagga acaccgacag gatggcgaag ccgtacagga
    10981 tcatggcctg cgcgccggcc agcttttcct cgcgcgactg gcccgtccat tcgtagccga
    11041 atccctgcgg cagctgcgcc accagacgct ccatctcggc catcgcggcg ccggtgctgt
    11101 agccgggcgc gggagagccc gaaatccgca tcgccgggta gccgttgtag cgcacggtct
    11161 gcatcgcgcc gctgatccag cgggtggacg cgaacgccga cagcggcacc gccttgccct
    11221 ggttgttgct ggcggtgagc cgcagcaggt cggccggctg catgcgcgcc ggtgcgtcgg
    11281 cctgcaccac cacgcgctgc aggcggccgc ggttcggaaa atcgttgatg tagctggaac
    11341 ccagcgccgt ggacagggtc gagttgatcg cttcgaacgg caccccgagc gcattggcct
    11401 tgtcgcggtc gatgtcgatc tgcagctgcg gcgcgtcctc cagcccgtcg gggcgcacct
    11461 gcgtcagcac cttgctctgc cccgcgagtg caatcaactg gttgcgcgcg gcgatcagcg
    11521 ccgccgggcc tgcgccaccc cggtcctgca ggcggaaggt gatgcccgag gccgtgccca
    11581 gttcggaaat cggcggcggg ctcaagggga aaatgatggc gtcgcgaatg cccgacaacg
    11641 cgccgaaggc ccgcccggcg agcgcctgcg ccgactggcc atcgcctttg cgc
//
LOCUS       DASWIY010000369         7146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1351650, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000369 DASWIY010000000
VERSION     DASWIY010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351650"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..3328)
                     /locus_tag="VGA59_13185"
     CDS             complement(146..3328)
                     /locus_tag="VGA59_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9720675.1"
                     /translation="MNTTEPKEGFNLSKWALDHPALTRYLMVVLMLLGFAAYFQLGQD
                     EDPPFTFRAMVVRTYWPGATAQQVAEQVTDKIERALQEAPYADKIRSYSKPGESQIIF
                     QIRDSSKPGEVANVWYTVRKKIGDMRFALPGGIQGPFFNDDFGDVYGVIYALESDGFD
                     YAELKSFADDVRQQLLRVQDVAKVELFGVQDEKVYVEISQKRLAQLGLDIGQVLQQLG
                     QQNAVESAGAVQTPLDVVQVRVGGQFEAVEQLRAMPIRGSSGNQLRLGDIATISRGYA
                     DPPAVKVRHQGKEVIALGVSMAKGGDIIRLGQSLKAVSHRVEDGLPAGIKLVQIQDQP
                     RAVANSVNEFVKTLVEAIAIVLAVSFVALGLHKRPDAALLPLWKRWYVDMRPGLVVLI
                     TIPLVLAMTFLAMDFWGIGLHKISLGSLIIALGLLVDDAIIAVEMMVRKMEEGYDKVR
                     AATFAYEITAMPMLTGTLITATGFLPIGMARSTVGEYTFAIFAVTVIALVLSWIVSVY
                     FVPYLGTLLLKPKPVVSEHHEHFDTPFYRTFRRTVDWCVEHRWITIGATLLTFALGIA
                     GMGKVQQQFFPDSSRPEIMVDIWFPEGTSFAANEETTRRVEQRLLREAGVSSVSTWVG
                     SGVPRFYLPLDQVFPQTNVSQFIIQPADLKVRESLRVKLPGLLANEFPEVRGRVKLLP
                     NGPPVPYPVQFRVVGADPVALRERADEVRAVMSKNPNMRGVNDNWNESVKVLRLEVDQ
                     PKARALGVTSQSIAQAARTILSGTNVGQFREGDKALDIVLRQPLDERNAITDIANAYL
                     PTASGRSIPLTQIAKPVFTWEPGVMWRENRDYAITVQGDVIEGLQGATVTNELLPELK
                     ALEAKWPLGYAIQVAGAVEESSKGSSSIAAGLPVMLFITFTLLMLQLHSFSRAMLVFL
                     TGPLGIAGVAGALLALGRPFGFVALLGVIALMGMIMRNSVILIDQIEQDRAKGVPTWD
                     AIVESAVRRLRPIVLTAAAAVLAMIPLSRSVFWGPMAVAIMGGLIVATVLTLLALPAM
                     YAAWFRVKRPDVEWRESPDPRGYSALSLQA"
     gene            complement(3337..4533)
                     /locus_tag="VGA59_13190"
     CDS             complement(3337..4533)
                     /locus_tag="VGA59_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9720676.1"
                     /translation="MSKHPSDRLARITLGLALAASAAVVACSKPEAPQEPVRSVKVLT
                     VGVASFQSGHEFAGEVRPRVESRLGFRVPGKIIRRQAELGQRVKSGQVLAQLDARDYQ
                     LAADAARAQVAAAGTNRDLAAADFKRYAALKEQNFISGAELERREATLKAAQAQWDQA
                     RAQLASQGNQAGYTQLVADVSGVITAVEAEPGQVVTAGMPVVRIAQDGPRDAVFSVPE
                     DKVAAIKPGSAVSVRVWADSTALAGKVREVAASADPVTRTYAVKVGLDAAATPALGAT
                     VYVSPQALSHAGMPVIKLPTSALRKDGQGTAVWVLEKSTMTLASQPVQVASADGNEAV
                     ISAGLQPGVLVVSAGVHVLSAGQTVAIYQEKTAQSTAVAAQDAARNLATASGTAHAAT
                     LPPAGK"
     gene            4893..5906
                     /locus_tag="VGA59_13195"
     CDS             4893..5906
                     /locus_tag="VGA59_13195"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX9720677.1"
                     /translation="MLGLLRGVSRSFYVSIRLLPAPLRRPVALAYLLARATDTLADTA
                     DLPPSQRPEKLEALAAAIDGRLPAAAMAASLASSAHLQHDVAERNLILALPWCLSWLD
                     DLAPADRDDVRAVLRHITYGQALDIERFAQGAPPMALQTAAQLDEYTYLVAGCVGEFW
                     TELCFRHLPGFAALPKHELRELGRDYGMGLQLVNILRDAGADLAAGRCYFPADELAAA
                     GLRPRDILAEPDRFDPVWHRWSALAERRLELGMRYAYAVGHRRVRAAGALPALVGMRT
                     LALLRLAGPQRLHRTVKVPRREVRSMMARLLFTLAGRAPLRALFGRLQGPPPGGGWEN
                     PRP"
     gene            5903..6742
                     /gene="hpnD"
                     /locus_tag="VGA59_13200"
     CDS             5903..6742
                     /gene="hpnD"
                     /locus_tag="VGA59_13200"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEX9720678.1"
                     /translation="MTPQQYVQEKAAASGSSFYYAFLFLPKPRRAAITAFYAFCREVD
                     DVVDNVSDSAVARTKLAWWQLEVAKSFGGQATHPVMKALMPCVPVFGIEQAHLQAVID
                     GCRMDLDQTRYLDFPALERYCHLVAGVVGEVSARIFGQADPRTTEYAHKLGLAFQLTN
                     IIRDVGEDAMRGRIYLPLAELQQFEVKAHEILNRKYSQRFAVLMKFQAERAHRLYDQA
                     LGQLPAVDRRSQKPGLMMANIYRTLLREIEHDGFQVLHQRVSLTPLRKFWLAWKVQAL
                     GKM"
     gene            complement(6788..>7146)
                     /locus_tag="VGA59_13205"
     CDS             complement(6788..>7146)
                     /locus_tag="VGA59_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720679.1"
                     /translation="ELQRGNPEKALELFRESLPLHRNYPTSPWITKGLAHLMIAYAAC
                     KRWRTAARLSGLLAKEGAGAPAAVPAELSGRVAKAFSDAILLTRAALGEQAFSAESGA
                     GRRMAREEGIRFALSD"
BASE COUNT         1185 a         2443 c         2308 g         1210 t
ORIGIN      
        1 ctagcccgca atgacaaaga taggctgtca tcccggactt gatccgggat ccatgaatcc
       61 atggattgtg gttcaagccc gcaatgacaa agataggctg tcatcccgga cttgatccgg
      121 gatccatgaa tccatggatt gtggttcaag cctgcaatga cagagctgaa tagccgcgcg
      181 ggtcgggcga ctcgcgccac tcgacgtccg gccgcttcac cctgaaccat gccgcataca
      241 tcgccggcag cgccagcaag gtgagcacgg tcgcgacgat caggccgccc atgatcgcga
      301 ccgccatcgg cccccagaac accgaccgcg acagcgggat catggccagc accgccgccg
      361 cggcggtcag cacgatgggc cgcagccgcc gcacggccga ttcgacgatc gcgtcccagg
      421 tcggaacacc tttggcgcgg tcctgctcga tctggtcgat caggatcacc gagttgcgca
      481 tgatcatgcc catcagggcg atcacgccca gcagcgccac gaagccgaag ggccggccca
      541 gcgccagcag ggcacccgcc accccggcga tgccgagcgg gccggtcagg aacaccagca
      601 tggcccggct gaaactgtgc agctgcagca tcagcagcgt gaaggtgatg aacagcatga
      661 ccggcaaccc ggcggcgatc gatgacgagc ccttcgagct ttcttccacc gctccggcga
      721 cctggatcgc atagccgagc ggccacttcg cctcgagtgc cttgagctcg ggcagcagtt
      781 cgttcgtcac tgtcgcgcct tgcaagccct cgatgacatc accctgcacc gtgatcgcgt
      841 agtcgcggtt ctcgcgccac atcacgcccg gctcccaggt gaacaccggc ttggcgatct
      901 gggtcagcgg gatcgagcgg cccgatgccg tcggcaggta ggcattggcg atgtcggtga
      961 tcgcgttgcg ctcgtccagc ggctggcgca gcacgatgtc cagcgctttg tcgccctcgc
     1021 ggaactggcc gacgttggtg ccggacagga tggtcctggc cgcctgcgcg atggactggc
     1081 tggtcacgcc cagcgcccgt gccttgggct ggtccacttc caggcgcagc accttgaccg
     1141 attcgttcca gttgtcgttg acgccgcgca tgttcggatt cttgctcatg accgccctga
     1201 cttcgtcggc gcgctcacgc aatgcgacgg ggtcggcacc tacgacacgg aactgcacgg
     1261 gatagggcac gggcggcccg ttgggcagca gcttcacccg gccgcgcacc tccgggaact
     1321 cattggccag cagtccggga agtttgaccc gcagcgactc gcgcaccttc aggtcagccg
     1381 gctggatgat gaactgcgac acgttggtct gcggaaagac ctggtccagc ggaaggtaga
     1441 agcgcggcac gcccgatccg acccaggtgc tgaccgagct gacgccggct tcgcgcagca
     1501 ggcgctgctc cacgcgacgg gtcgtctctt cgttggcggc gaacgaggtc ccttccggga
     1561 accagatgtc caccatgatc tccggccggc tggagtcggg gaagaactgc tgctgcacct
     1621 tgcccatgcc ggcgatgccc aacgcgaagg tcagcagtgt ggcgccgatg gtgatccagc
     1681 gatgctccac gcaccagtcc accgtgcgcc ggaacgtgcg gtagaacggg gtgtcgaaat
     1741 gctcgtggtg ctcgctgacc accggcttcg gtttcaacag caatgttccc agatagggca
     1801 cgaaatagac ggagacgatc caactgagca cgagcgcgat caccgtgacc gcgaagatgg
     1861 cgaaggtgta ctcgcccacc gtcgatcggg ccatgccgat cggcaggaat ccggtggcgg
     1921 tgatcagggt gccggtcagc atcggcatgg ccgtgatctc gtaggcgaaa gtcgcggcgc
     1981 ggaccttgtc gtagccctct tccatcttgc gcaccatcat ctccacggca atgatggcgt
     2041 cgtccaccag caggcccagc gcgatgatga gcgagcccaa cgaaatcttg tgcaggccga
     2101 tgccccagaa gtccatcgca aggaacgtca tcgcgagcac cagcggaatc gtgatgagca
     2161 ccaccaggcc cgggcgcatg tccacgtacc agcgtttcca caagggcagc aacgcggcgt
     2221 cgggccgctt gtgcaggccc agcgcgacga agctcaccgc cagcacgatg gcgatggctt
     2281 cgaccagcgt cttgacgaat tcgttgaccg agttcgctac cgcgcgcggc tggtcctgga
     2341 tctgcaccag cttgatgccg gcgggaaggc cgtcctcgac gcggtgggac acggccttga
     2401 gcgactggcc caggcggatg atgtcgcccc ccttggccat cgaaaccccc agggcgatga
     2461 cttccttgcc ctggtgccgc accttgaccg ccggcgggtc ggcatagccg cgcgagatgg
     2521 tggcgatgtc gcccaggcgc agctggttgc ccgagctgcc gcgaatcggc atggcccgaa
     2581 gctgttccac agcctcgaac tggccgccga cccgcacctg caccacgtcc agcggcgtct
     2641 gcaccgcgcc ggcggattcc accgcgttct gctgtcccag ctgctgcagg acctggccga
     2701 tgtccagccc gagctgggcc aggcgctttt gcgatatctc gacgtagacc ttctcgtcct
     2761 gcacgccgaa cagttcgacc ttggccacgt cctgcacccg cagcagctgc tggcgcacgt
     2821 cgtcggcgaa agacttgagc tcggcgtaat cgaagccgtc cgattcgagc gcatagatca
     2881 ccccatagac atcgccgaag tcgtcgttga agaacgggcc ctggatgccg ccggggagcg
     2941 caaagcgcat gtcgccgatc ttcttgcgca ccgtgtacca gacgttggcc acctcgcccg
     3001 gcttcgacga gtccctgatc tggaaaatga tctgcgactc gccgggcttg gagtaactgc
     3061 ggatcttgtc ggcgtagggc gcctcctgca gcgcccgctc gatcttgtcg gtgacctgtt
     3121 cggccacctg ctgcgcggtg gcgccgggcc aataagtacg caccaccatc gcgcggaacg
     3181 tgaacggcgg gtcttcgtcc tgccccagct ggaagtaggc ggcgaaaccc agcagcatca
     3241 acacgaccat caggtagcgc gtgagcgcgg gatggtcgag cgcccatttg gagaggttga
     3301 acccctcctt cggttccgtc gtgttcatgc cggccctcac ttgcctgcgg ggggtaaggt
     3361 ggcggcgtgg gcggtgccgg acgcggtggc cagattcctt gcggcgtcct gtgccgcgac
     3421 cgccgtgctc tgagccgtct tctcctggta gatggccacc gtctggccgg ccgaaaggac
     3481 atgcactccc gccgacacca ccagcacgcc cggttgcagg cccgccgaga tcaccgcctc
     3541 gttgccgtcc gcgctcgcga cctggaccgg ctgcgacgcc agcgtcatgg tggacttttc
     3601 cagcacccag accgccgtgc cctgcccgtc cttgcgcagc gcgctggtcg gaagcttgat
     3661 cacgggcatg ccggcatggc tcaacgcctg cggcgacacg taaaccgtcg cacccagcgc
     3721 gggggtcgcg gcggcatcga gccccacctt caccgcataa gttcgcgtca ccggatcggc
     3781 gctcgccgcg acctcgcgca ccttgccggc gagcgccgtg ctgtcggccc agacccgcac
     3841 ggaaacggcg gacccgggct tgatcgccgc gaccttgtct tccggcaccg agaacaccgc
     3901 atcgcgcggg ccatcctgcg cgatgcgcac caccggcatg cccgccgtga cgacctggcc
     3961 cggctcggcc tcgacggccg ttatcacgcc cgacacatcg gccacgagtt gggtgtaccc
     4021 cgcctggttg ccctgcgagg ccagctgcgc ccgggcctgg tcccattggg cctgcgccgc
     4081 cttcagcgtg gcctcgcgcc gctcgagctc ggcgccactg atgaagttct gttccttcag
     4141 ggccgcataa cgcttgaaat ccgccgcggc gaggtcacga ttggtgccgg ccgcggcgac
     4201 ctgggccctt gccgcgtcgg cggccagctg gtagtccctt gcgtccagtt gcgcgagcac
     4261 ctggccggac ttgacgcgct gccccagttc ggcctggcgc cggatgatct tgccgggcac
     4321 gcggaacccc agcctggatt cgacccgggg ccggacttcg cccgcgaatt catggcccga
     4381 ctggaagctc gcgacgccga cggtgagcac tttgacggac cgcaccggct cctgcggcgc
     4441 ctcgggtttg ctgcaagcca ccaccgccgc cgatgcggca agggcgagtc cgagcgtaat
     4501 gcgggcaagg cggtcggaag gatgtttgga catggtcgag ctcagctgaa agtacacgaa
     4561 accagtcatt aatgactgac cggttagtaa tctaggagaa acgtggaggc ttgtcaagct
     4621 gcaattcacg agtcgccacg ccggtggatc gcggtttgag tccacccagc cgcctgccac
     4681 agggacttgg cctgggggtc ggttttcctt ggcggccacg atgctgtgat gtccggcttc
     4741 gctgccaaag tggggccgtg cgacgtcgct ccccaatgcc cttgtggtga aatcatccaa
     4801 gtttggaaac aggaaccgcc ctgggccgca atgagcatgc gtgtcgccaa ggcctgatca
     4861 gcatgcaaca ggcgcccacg tcccctaccg aaatgcttgg actcctgcgg ggagtctcac
     4921 gctcgttcta tgtttcgatc cgcctgcttc cggccccact gcgccgaccg gtcgcgctgg
     4981 cctatttgct ggcgcgcgca acggacacgc tggcggacac cgctgacctg cctcccagcc
     5041 agcggccgga gaaactggaa gcgctggcgg ccgcgattga tggccggctt cccgcggccg
     5101 ccatggccgc cagcctggcg tcgtccgcgc acctccaaca tgacgtggcg gaacgcaatc
     5161 tcatcctcgc cctgccctgg tgcctgtcgt ggctcgacga cctggcgccg gcggaccgcg
     5221 acgacgttcg cgccgtgctg cgccacatca cgtacggcca ggcattggac atcgagcgct
     5281 ttgcgcaagg cgcgccaccg atggcgctcc agacggcggc gcagctggat gagtacacct
     5341 acctggtggc cggctgcgtg ggcgagttct ggaccgagct gtgctttcgc cacctccccg
     5401 gctttgccgc gctgccgaag cacgagctgc gtgagctggg ccgcgactac gggatgggcc
     5461 tgcagctggt gaacatcctg cgcgatgcgg gggccgacct ggccgcgggg cgctgctatt
     5521 tcccggccga cgaactggcc gctgccggac tgcggccccg cgacatcctg gccgagcccg
     5581 acagattcga ccctgtctgg caccgctggt ccgcgctggc cgagcgccgg ctcgagctcg
     5641 gcatgcggta cgcgtacgcg gtaggccacc gccgcgtacg cgccgccggc gccctgcccg
     5701 cactggtcgg aatgcgcacg ctcgccttgc tgcggctggc gggtccgcag cgcctgcatc
     5761 gcacggtgaa ggtgccgcgc cgtgaagtcc gctccatgat ggcccggttg cttttcacgc
     5821 ttgcgggccg tgcgccgctg cgggcgctgt ttggccggtt gcaaggcccg ccccccggag
     5881 gcggatggga gaatccccgc ccatgacacc gcaacagtac gtgcaggaaa aggccgccgc
     5941 ctcgggcagc agcttctatt acgcgttcct gttcctgccc aagccgcggc gcgccgcgat
     6001 caccgccttc tatgcgtttt gccgcgaagt cgacgatgtc gtggacaacg tgagcgacag
     6061 cgccgtggcc cgcaccaagc tggcatggtg gcagctggaa gtcgcgaagt cgttcggcgg
     6121 ccaggcgacc catccggtga tgaaggccct gatgccatgc gtgccggtat tcggtatcga
     6181 acaggcgcac ctgcaggcgg tgatcgacgg ctgccggatg gacctggacc agacccgcta
     6241 cctcgatttc cccgccctgg aacgctattg ccacctggtg gccggtgtgg tgggcgaagt
     6301 ctcggcgcgc atcttcggcc aggccgaccc gcgcacgacg gaatacgcgc acaaactggg
     6361 actggcgttc cagctcacca acatcatccg cgacgtcggc gaggatgcga tgcgcggccg
     6421 catctacctt ccgctcgccg agctgcagca gttcgaggtc aaggcccacg agatcctcaa
     6481 ccgcaagtat tcgcagcgct ttgcggtgct catgaaattc caggcggagc gcgcgcatcg
     6541 gctgtacgac caggcactgg gccagttgcc cgccgtcgac cggcgcagcc agaaaccggg
     6601 cctgatgatg gccaatatct accgcacgct gctgcgcgag atcgagcacg acggcttcca
     6661 ggtgctgcac cagcgcgtga gcctgacacc gctgcgaaaa ttctggctgg cgtggaaggt
     6721 gcaggcgctg ggcaagatgt gaaccacgcc tgaacgggtg gatcctggat actccgcgcc
     6781 gccaggctca gtcggaaagt gcaaagcgta taccttcttc gcgtgccatg cgccgtcctg
     6841 caccgctctc ggcgctgaaa gcctgctcgc ccaaggccgc gcgggtgagc aggatggcat
     6901 cactgaaagc cttggctacg cgtcctgata actccgccgg gaccgcagca ggcgcgccgg
     6961 cgccttcttt tgccagcagt ccagacaggc gagcggctgt gcgccatctc ttgcacgccg
     7021 cgtatgcaat catgagatgc gcgagcccct tagtgatcca tggcgacgtg gggtagttgc
     7081 ggtgcagtgg caggctctcc ctgaatagct cgagcgcctt ctcggggttg ccgcgttgga
     7141 gttcca
//
LOCUS       DASWIY010000370         8039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1750665, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000370 DASWIY010000000
VERSION     DASWIY010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750665"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VGA59_13210"
     CDS             complement(<1..442)
                     /locus_tag="VGA59_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEX9720680.1"
                     /translation="MAASLDNKLVVAISSRALFNFEEENAIFESGDPQAYMKLQLERL
                     DLPARPGIAFSLIKKLLAFNDTTGQRVEVVILSRNDPVSGMRIFRSGHANEIKLERGV
                     FTQGRSPFRYLRPLRAHLFLSANLEDVREALAAGFPAARVLTESV"
     gene            complement(520..1752)
                     /gene="gspF"
                     /locus_tag="VGA59_13215"
     CDS             complement(520..1752)
                     /gene="gspF"
                     /locus_tag="VGA59_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEX9720681.1"
                     /translation="MPAYSFEALDAQGQTRRGVMEADTAKAVRSQLRAQALVPMQVVP
                     VSAGVAANEKYFLWSGGAFSRGVFSATGLAIWTRQLAGLITSGLPLERALTALTDEAE
                     NDRERDLVAALRAEVNAGSPFGKALARFPREFPDIYTAVVGAGEQSGNLGLVLERLAD
                     DLEERQALQAKLMGAALYPAIVTVVAIAIVLFLVAYVVPQVANVFAGSKRALPFLTVA
                     MLAVSGLVRNWGWLAVLLAVAGTIAARLALRNAATRERFDAAWLKLPLVGRLARGYNA
                     ARFASTLAMLAGAGVPILKALQAAAETLGNRAMRADAMDALVLVREGAPLASAMAQKK
                     RFPGLLSMFARLGEQTGQLPAMLQRAANQLAAEVQRRAMQLATILEPLLIVAMGLVVM
                     LIVLAVLLPIIQLNQFAR"
     gene            complement(1827..3437)
                     /locus_tag="VGA59_13220"
     CDS             complement(1827..3437)
                     /locus_tag="VGA59_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9720682.1"
                     /translation="MPAQQDRFVHDRLPPRSQWPRLIYDRPEFQFPAQLNLVEELLDK
                     AQAKGFGARPLLRSSSVALSYTQARERVDRICRVLVEDLGLVPGNRVLLRGGNSIDLA
                     LAWLAVVKAGMIAVATMPLLRARELGDIIDQAQPTVALCDVKLLEELELARKERPVLG
                     TVIAFNTPEQPGSLAVRAAPKKGDFAPCATAADDIALLAFTSGTTGRPKAAVHTHRDV
                     LAACEAWPRHVLRATPEDIVVGSPPLAFTFGLGGLLMFPMWAGASVYFPDAPYTPETA
                     VRMIVETGATICYTAPTFYRQMAPFAREHGVGRLRICVSAGEGLPDATRQLWKQATGI
                     EMLDGIGATEMFHIFISSGGSEVRPGAIGKVVPGYQAKVVDDDGREVPRGTIGKLAVI
                     GPTGCKYLDDPRQANYVKDGWNYPGDAFTEDEDGYFFYQARADDMIITSGYNVGGPEV
                     EDALLKHPAVAECGVIGKPDTQRGMIVKAFCVLKPGHAGDAAMVKALQDHVKASIAPF
                     KYPREIEFVAGLPRTETGKLQRFKLRQL"
     gene            complement(3464..3946)
                     /locus_tag="VGA59_13225"
     CDS             complement(3464..3946)
                     /locus_tag="VGA59_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9720683.1"
                     /translation="MDLEARAHSEHPEALRLWLRLLTCTQIVEKQVRTQLRERFDTTL
                     PRFDLMAQLERAPDGLKMNELSRRMMVTGGNVTGITDQLVAEGLVDRVDVEGDRRAYR
                     VRLTPKGRKLFHEMANQHESWVVEAFAGLSDKDVATLHKLLGKVKDHALSRSRAEATA
                     "
     gene            4138..5400
                     /locus_tag="VGA59_13230"
     CDS             4138..5400
                     /locus_tag="VGA59_13230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9720684.1"
                     /translation="MDTLIRKIESQLAGLPVPVALQLPAGERVGPPTAAVTLSFKDWS
                     SLATMAAGQIGRMAEDFVESRVQLEGRMRDLMAVAARLLPGSPVSSDSGWWTQMLRRA
                     KSVAAHSTHKDAQQVQFHYDVSDDFYALWLDPRRVYSCAYYRDMSMSLAQAQEAKLDQ
                     ICRKLMLKPGERFLDIGAGWGGLLMWAAENYGVNATGITLSKNQHAHVQRLIEDERLQ
                     GRVRMELRDYRELGESEGFDKIASVGMFEHVGQANMPAYFGKIHRLLKPGGLVMNHGI
                     TSGGIHPGHLGAGMGEFVEKYIFPGGEVLHISHVLKEMAAAGLEMVDTENLRPHYART
                     LWGWSDALEARLEDAQRVLATTGHADDAAKILRAYRLYLAGSAMIFEQGWIGLHQILA
                     IRPDGQLHTGALRGAQSAYPFNREYMYR"
     gene            5407..5730
                     /locus_tag="VGA59_13235"
     CDS             5407..5730
                     /locus_tag="VGA59_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HEX9720685.1"
                     /translation="MLFKFKSKAAGDLIMLEPNGRRVLEIIGKEALPKGIILTEQMPA
                     AMAALEAAIAREEADQKAMVEDANAKGEVPPKFEAVSLRQRAVPFLDMLRRCEKAGQE
                     IVWGV"
     gene            complement(6036..6542)
                     /locus_tag="VGA59_13240"
                     /pseudo
     CDS             complement(6036..6542)
                     /locus_tag="VGA59_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
     gene            complement(6683..7672)
                     /locus_tag="VGA59_13245"
     CDS             complement(6683..7672)
                     /locus_tag="VGA59_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9720686.1"
                     /translation="MKKLFHSLIALTAVATAIGVQAQAFPIKDKPVTIVVPFAAGGPT
                     DRVARDLAEAMRKPLGGATVVVENAAGAGGTIGANKVAKASADGHTLLLYHIGMATSP
                     ALYRNLPYKTLDDFEYVGLLNEVPMTLIGRPSLPANNYAELRTWINANKGKINLANAG
                     LGAASHLCGLLFMNALKMEMTTVPYKGTGPAMTDLIGGQVDLMCDQTTNTTGQIDAKK
                     VKAYAVTTTKRLTTPALKDLPTLQEAGLKGFEVTIWHGLYAPKRTPPDALKKINAALK
                     AAMKDPDFIKKQEGLGAVLVTDKRIEPAEHKKFVAAEIAKWGPIIKAAGVYAD"
     gene            complement(7847..>8039)
                     /locus_tag="VGA59_13250"
     CDS             complement(7847..>8039)
                     /locus_tag="VGA59_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEX9720687.1"
                     /translation="VSGEMANDVMTLQVETTSVPNGLDERIGDAIRDITKLRGDVQLL
                     RPGSLPNDGKVIEDARSYK"
BASE COUNT         1301 a         2614 c         2742 g         1382 t
ORIGIN      
        1 aaacggactc ggtcagcacc cgtgcggccg ggaagcccgc ggccagcgcc tcgcgcacgt
       61 cctcgaggtt ggccgagagg aacaggtgcg cgcgcaaggg ccgcaggtag cggaagggcg
      121 agcggccctg cgtgaagacg ccgcgttcca gcttgatctc gttggcatgg cccgagcgga
      181 agatgcgcat gccggatacc gggtcgttgc gcgaaaggat cacgacttcc acgcgctggc
      241 cggtggtgtc gttgaaggcc agcagtttct tgatcaggga aaaggcgatg cccgggcgcg
      301 cgggcaggtc cagccgctcg agctgcagct tcatgtaggc ctgcggatcg ccgctttcga
      361 agatcgcgtt ctcctcctcg aagttgaaga gcgcgcgcga ggagatcgcg accaccagct
      421 tgttgtcgag cgaggcggcc attcagactc cgggcttttc cccctctccc tctgggagag
      481 ggcaggggtg agggctggtg cgcaaatggg atgactgtgt catctggcga attgattgag
      541 ctggatgatc ggcagcagca ctgcgagcac gatcagcatg accaccaggc ccatcgccac
      601 gatcagcagc ggctccagga tcgtcgccag ctgcatggcg cggcgctgca cctcggcggc
      661 cagctggttg gccgcgcgct gcagcatcgc gggcagctgg ccggtctgtt cgcccagccg
      721 cgcgaacatc gacagcaggc cgggaaagcg cttcttctgg gccatcgcgg aagccagggg
      781 cgcgccttcg cgcaccagca ccagcgcgtc catggcgtcg gcgcgcatgg cccgattgcc
      841 cagggtttcc gcggcggcct gcagcgcctt gaggatggga acgccggcac cggccagcat
      901 cgccagagtg ctggcgaagc gcgcagcgtt gtagccgcgc gcaagccggc ccaccagcgg
      961 cagcttcagc caggccgcat cgaagcgctc gcgcgtggcg gcgttgcgca gcgccagccg
     1021 cgcggcgatg gtgcccgcca ccgcgagcag gaccgccagc cagccccagt tgcgcaccaa
     1081 gccgctgacg gccagcatgg ccaccgtgag gaacggcagg gcgcgcttgc tgccggcgaa
     1141 cacgttggcg acctggggca cgacataggc caccaggaac agcacgatgg caatcgcgac
     1201 gacggtgacg atggccggat aaagggcggc gcccatcagc ttggcctgca gggcctggcg
     1261 ctcttccagg tcgtcggcca gccgctccag gaccagcccc aggttgccac tctgctcgcc
     1321 ggcgccgacc accgcggtat aaatatcggg gaattcgcgc gggaaccgtg ccagcgcctt
     1381 gccgaagggc gagccggcgt tgacttcggc gcgcaacgcg gccaccaggt cgcgctcgcg
     1441 atcgttctcg gcttcgtcgg tgagggcggt gagggcgcgt tccagcggca gtccggacgt
     1501 gatcaggccg gccagctggc gggtccagat cgccagcccc gtggcgctga aaacgccgcg
     1561 gctgaaagcg ccgccgctcc acaggaagta cttttcattg gccgcgaccc cggcgctcac
     1621 cggcacgacc tgcatcggca ccagcgcctg cgcgcgcagc tggctgcgca cggccttggc
     1681 ggtgtccgcc tccatcacgc cgcggcgggt ctggccctgc gcgtccaggg cctcgaatga
     1741 ataagctggc attccgggat tatctgccgg ttgccttcat cgggcggcgc gccgggaaag
     1801 acctgccgag gccgggccgg ccgtgttcag agctggcgca gcttgaaacg ctggagcttg
     1861 ccggtctccg tgcgcggcag tccggccacg aattcgatct cgcgcgggta cttgaatggc
     1921 gcgatcgatg ccttgacatg gtcctgcagt gccttgacca tcgcggcgtc gcccgcatgg
     1981 cccggcttga gcacgcagaa agccttgacg atcatgccgc gttgcgtgtc gggcttgccg
     2041 atcacgccgc actcggccac ggccgggtgc ttgagcaagg cgtcctcgac ctcgggcccg
     2101 cccacgttat agcccgaggt gatgatcatg tcgtcggccc gcgcctggta gaagaagtag
     2161 ccgtcctcgt cctcggtgaa tgcgtcgccg ggatagttcc agccgtcctt cacgtagttg
     2221 gcctggcgtg gatcgtccag gtacttgcag ccggtgggcc cgatcaccgc cagtttgccg
     2281 atcgtgccgc gcggcacttc gcggccgtcg tcatccacga ccttggcctg gtagcccggc
     2341 acgaccttgc cgatggcgcc gggccgcact tcgctgccgc ccgacgagat gaagatatgg
     2401 aacatctcgg tcgcgccgat accgtccagc atctcgatgc cggtggcctg tttccacaac
     2461 tggcgggtgg cgtccggcag cccttcgccg gcactgacgc agatgcgcag gcgtcccacg
     2521 ccatgctcgc gcgcgaacgg cgccatctgg cggtagaacg tgggagccgt gtagcaaatg
     2581 gtcgcgccgg tctccacgat catccgcacc gcggtctcgg gcgtgtaggg cgcgtcggga
     2641 aaatagaccg aggcgccggc ccacatcggg aacatcagca ggccgcccag gccgaacgtg
     2701 aaggccagcg gcggcgagcc caccacgatg tcctcggggg tcgcgcgcaa gacatggcgc
     2761 ggccaggcct cgcaagccgc gagcacgtcg cgatgggtat ggacggcggc tttcggccgg
     2821 ccggtcgtgc ccgaagtgaa cgccaacagg gcgatgtcgt cggcggccgt cgcgcaaggc
     2881 gcgaaatcgc ccttcttcgg cgcggcgcgc acggccagcg agccgggctg ttccggcgtg
     2941 ttgaaggcga tgaccgtgcc gagcaccggc cgctccttgc gcgccagttc cagctcctcg
     3001 agcagcttga cgtcgcacag cgcgaccgtc ggctgggcct ggtcgatgat gtcgcccagt
     3061 tcgcgcgcgc gcagcagcgg catggtcgcc accgcgatca tgccggcctt gaccacagcc
     3121 agccacgcca gcgccaggtc gatcgaattg ccgccgcgca gcagcacccg attgccgggc
     3181 accagaccca ggtcctcgac caggacgcgg cagatgcgat cgacccgctc gcgcgcctgc
     3241 gtgtaactca aggcgacact gctggagcgc agcagcgggc gggcgccgaa accctttgcc
     3301 tgcgccttgt ccagcaactc ttcgaccagg ttcagctggg ccgggaactg gaattccggg
     3361 cgatcgtaga tcagccgggg ccactgcgac cgcggcggaa ggcggtcgtg gacgaatcgg
     3421 tcttgctggg cgggcatgcc tgtgctcctt tacatccttg ccgtcaagcc gtcgcttctg
     3481 cgcgagagcg tgacagggcg tggtccttga ccttgcccag cagcttgtgc aacgtggcga
     3541 cgtccttgtc gctcaagccg gcgaaggcct cgacgaccca gctctcatgc tggttggcca
     3601 tctcgtgaaa cagcttgcgc cccttggggg tcaggcgcac gcggtaggcg cgccggtcgc
     3661 cttccacgtc gacgcggtcg accaggccct cggcgaccag ctgatcggtg atgccggtca
     3721 cgttgccgcc ggtcaccatc atgcggcgcg acagctcgtt catcttcagg ccatccggcg
     3781 cgcgctccag ctgggccatg aggtcgaatc gcggcagggt cgtgtcgaag cgctcgcgca
     3841 actgcgtgcg cacctgcttt tcgacgatct gggtacaggt gagcaggcgc agccacagcc
     3901 gcagggcctc ggggtgttcg ctgtgcgcgc gcgcttccag gtccatcggc gggttccttt
     3961 gcatcgtcta aatagtttag gcctaaagaa aacgctgtca actcgaccta tcccgcgccg
     4021 cgcggctgcc cgatgcggcc gcaaccaccg cgctaccgtg caaagcgaaa tcgtcctaca
     4081 cttgcgttct tactccctgg gttgacgggc gccttgaaca gaggcaggaa tttgctcatg
     4141 gacacactta ttcgcaagat cgagtcgcag ctggccggtt tgccggtgcc ggtcgcgctg
     4201 cagttgcccg ccggcgaacg cgtggggccc ccaacggcag ccgtgacgct ctcgttcaag
     4261 gactggtcca gcctggcgac catggcggcc ggccagatcg gccggatggc tgaggatttc
     4321 gtcgagagcc gcgtccagct ggaagggcgc atgcgcgact tgatggcggt cgcggcgcgc
     4381 ttgcttccgg gcagcccggt gagcagcgac agcggctggt ggacgcagat gctgcgccgg
     4441 gcgaaatcgg tggcggccca ttcgacccac aaggacgcgc aacaagtgca gttccactac
     4501 gacgtgtccg acgattttta cgccctgtgg ctcgatcccc ggcgggtgta ttcctgcgcc
     4561 tactaccgcg acatgtcgat gtcgctggcc caggcccagg aggccaagct cgaccaaatc
     4621 tgccgcaaac tcatgctcaa accgggggaa cgcttcctgg atatcggcgc gggctggggt
     4681 gggctgttga tgtgggccgc ggagaattac ggcgtcaacg ccaccggcat cacgttgtcg
     4741 aagaaccagc atgcgcacgt gcagcggctg atcgaagacg agcggctgca gggcagggtg
     4801 cgcatggaac tgcgtgacta ccgggaactg ggcgagtcgg aaggtttcga caagatcgcc
     4861 tcggtgggca tgttcgagca tgtcgggcaa gccaacatgc cggcgtattt cggcaagatc
     4921 caccggctgc tcaagcccgg cggcctggtc atgaaccacg gcatcacgtc gggtggcatc
     4981 caccccggcc acttgggcgc ggggatgggc gagttcgtcg agaaatacat ctttcccggc
     5041 ggcgaggtgc ttcacatcag ccatgtcctc aaggaaatgg cggcagcggg actggaaatg
     5101 gtcgacaccg aaaaccttcg gccgcattac gcccggacct tgtggggctg gtcggacgcc
     5161 ctggaggcgc gcctggagga cgcccagcgc gtgctggcca ccaccggcca cgcggacgat
     5221 gcggcgaaga tcctgcgcgc ataccgcctt tacctcgccg gtagcgcgat gattttcgag
     5281 caaggttgga tcgggctgca ccagatcctt gcgatccgcc cggatggcca gttgcacacc
     5341 ggcgcgctga ggggcgcaca atcggcctat cccttcaacc gcgagtacat gtaccgctag
     5401 gcatcgatgc tattcaagtt caagtccaaa gccgccggcg acctgatcat gctggagccc
     5461 aatgggcgcc gcgtgctcga aatcatcggc aaggaggccc tgcccaaagg aatcatcctg
     5521 accgagcaga tgcccgccgc catggccgcc ctggaagcgg cgatagcgcg ggaggaggcg
     5581 gaccagaagg cgatggtcga agacgccaat gccaagggag aggttccgcc aaaattcgaa
     5641 gccgtctccc tgcgccagcg cgccgtgccc tttctggaca tgctgcgccg ctgcgaaaaa
     5701 gccggccagg agatcgtctg gggggtgtag ggctgtcatt gcgggctggg ctcgcgggga
     5761 acgcccgggg cgcgcagctg tccagtgcag cgagcccagt gtcgggccta cgctatgcac
     5821 gcgccgaggc atcaagctcc ggcccggccc tgcaatccat gaattcaggg gtcgtggatc
     5881 ccggatcaag tccgggatga caaccccgta cctttgtcat tgcgggctcg acccgcaatc
     5941 caggaagtct tggatcccgg atcaggcctg ccccggatcc ggggtccggg atgacgggaa
     6001 ctatccggga tcgggatacg gccgctggcg cagcgtcaat cgatcttcgc gcctgaagcc
     6061 ttcaccaccg gctcccattt cttgtgctcg gcctggatgt ggcgcgcgaa ctcggccggc
     6121 gtgttgccgc tcgggatcgc gccgtgggaa agcagctttt ccttgatagc gggcgtgttc
     6181 agcgccttcg ccacttcctg ctggatgcga ttcacgatat ccggcggcgt accggcgggc
     6241 gccagcaggc cgaaccatga agtcgcgtcg aaccccttga ggccggcggc ttcctcgatg
     6301 gtggggacgt cgggcagcgc ggccgagcgt tgccggctcg tcaccgccag cgccttgagc
     6361 ttgcccgcct tgatctgggg catcgacgag ggcaggttgt cgaacatcac atccatggtg
     6421 ccgcccacca tgtccagcat cgccggtccc gagccgcggt aggggaagtg gaccatgtag
     6481 gtgccggtcc tgctcttgaa cagctcgccg gccaggtgga tcgaagtgcc gtttccgctt
     6541 gaaggatcag catcttccgt gcgtcgcgcc gcggctgtcc agcgttggtc ctgcacagcg
     6601 gaaccggcgg cttgcggcca ggggcgcacg accagtgaaa aggccgcccg aaggcggcct
     6661 gcgaatcgct gcgaacgcgc tttcagtcgg cgtaaacgcc ggcagccttg atgatcggac
     6721 cccatttggc gatctcggcg gcgacgaatt tcttgtgctc ggccggctcg atccgcttgt
     6781 cggtcacgag gaccgcgccc aggccctcct gcttcttgat gaagtccggg tccttcatcg
     6841 cggccttgag cgcggcgttg atcttcttca gggcgtcggg gggcgtccgc ttgggggcgt
     6901 acaggccgtg ccagatggtg acttcgaagc ccttgaggcc ggcttcctgc agcgtcggca
     6961 gatccttcag tgccggggtg gtcaggcgct tggtcgtggt gacggcgtag gccttgacct
     7021 tcttggcgtc gatctggccg gtggtattgg tggtctggtc gcacatcagg tcgacctggc
     7081 cgccgatcag gtcggtcatg gccggtcccg tacccttgta cggaaccgtt gtcatctcca
     7141 tcttgagggc attcatgaac agcaggccgc acaggtggga ggcagcgccc aggccggcgt
     7201 tggccaggtt gatcttgccc ttgttggcgt tgatccacgt gcgcagctcc gcgtaattgt
     7261 tggccggcag cgaggggcgg ccgatcagcg tcatggggac ttcgttgagc aggccgacgt
     7321 actcgaagtc atccaacgtc ttgtagggca agttgcggta cagcgcgggc gacgtggcca
     7381 tgccgatgtg atacagcaac agcgtgtggc cgtccgcgct ggccttggcc accttgttcg
     7441 cgccgatggt gccgccggcg ccggccgcgt tttcgaccac gacggtggcg cccccgagcg
     7501 gcttgcgcat ggcttcggcc aggtcgcgcg cgacccggtc ggtcggtccg ccggcggcga
     7561 agggcaccac aatcgtgacg ggcttgtcct tgatggggaa ggcctgcgcc tgcacgccaa
     7621 tagcggtggc cacggcggtc agcgcgatga gggaatggaa cagctttttc atgggtcaag
     7681 actcctgctt gggtgacgtg ggaatcctaa gccccgacat atagtggcgc atcgcgggga
     7741 actacgtagc tcaagctggg gaaattccct aagctcgacc atttcttatg gcgtatggcg
     7801 caggtccgcg tggaccgggc gcgagggccc tcgatgcgct agcgcctcac ttgtagctgc
     7861 gtgcgtcctc gatgaccttg ccgtcattgg gcaagctgcc ggggcgcagc agctgcacat
     7921 cgccgcgcag cttggtgatg tcccggatgg catcgccgat gcgctcgtcc aggccatttg
     7981 gcacggacgt ggtttcgacc tgcaacgtca tcacgtcgtt cgccatttcg ccgctgacc
//
LOCUS       DASWIY010000371        16998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1000892, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000371 DASWIY010000000
VERSION     DASWIY010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 16998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16998
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000892"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..583
                     /locus_tag="VGA59_13255"
                     /pseudo
     CDS             116..583
                     /locus_tag="VGA59_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
     gene            708..3119
                     /locus_tag="VGA59_13260"
     CDS             708..3119
                     /locus_tag="VGA59_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9720688.1"
                     /translation="MAQDEFRHIGASHPRKEDERLLTGRGRYIDDIDVPGALHACFVR
                     SPHAHARIVGIDCRAALDMPGVAAVVTGKDLAAWTTRHRMAPAIEGLQPVEMDTLPID
                     KVRFQGDPVACVIAQDRYVAEDAAEQVAVDYEVLPAVTSMWQALDPASPRVDETLLSN
                     LLSHQHAGHGDVQARMGEAHRVVESTFSQQRQTHLPIETRGCIAVWDEGRRHLTFHVG
                     TQVPHPYRTTLAARLRLSESQVTVISPDVGGGFGQKIALYREELTVAAVARQLKAPVR
                     WREDRMENLLASSQAREDFCRTRAAVDAQGRLLALDLEIVEDFGAYSFYPGNYLARVV
                     AMILTGPYKVRDYSYDIKVLLSNKVGNGPMRAPMSITSWVMEGTMDAIASNLGLDPVA
                     VRRINMVEARDLPYTMATGEVLADITPRETLEAALAAIDYRAFRERQRAEREHGVYLG
                     LGICTVVESTTYGSRFYKSAGIPGSGHEAAWVRIEPSGAVNASCGLGATGQGYETSLA
                     NAVAEGLGVSPDCVEIHLGHTDIAPYGMGSRGARGGTAGGGTLYLCALDARAKVLAIA
                     ARCLGLAGSAGLRMCEGRIEQQAGSQWRDCGLSLADIARTAYLDPLALPPGMGPGLEF
                     HKTYEPPPMTYSNSTHVCEVRVEVATGAIRFERYLVAEDCGTVLSPVVVEGQQHGAIA
                     MGLSGALFEHVEYDDNGQNLSGSLADYLVATAHELPHIEILSMHTPNKATPAGIKGMA
                     EGGVMGAIGALTNAVNDALAPFGVVASQQPLTPMYLRGLLRGRQPSTVFTAPAPVDAQ
                     PATIE"
     gene            3132..4016
                     /locus_tag="VGA59_13265"
     CDS             3132..4016
                     /locus_tag="VGA59_13265"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9720689.1"
                     /translation="MTSTDNKARVGFIGLGIMGQSMAGHILAGGHPLAVYNRSRDKAA
                     ALLARGAQWCATPGEVAGQSDVVITMVGYPSDVEEVWLGPDGILSQANDALLIDMTTS
                     SPELARRIAIAAAGRRCQALDAPVSGGDIGARDAKLSIMVGGAREAFEDALPILQLLG
                     TNVVHQGGPGAGQHTKMCNQIVIASTIMGVCEGLAYGKAAGLDLPTVLKSIGAGAAGG
                     FQLNVFGAKIINGDFAPGFFIEHFVKDLGIALAEAQRMKLELPGLALARKLYMVLVEQ
                     GHARLGTQALYKYYGTSA"
     gene            4013..4633
                     /locus_tag="VGA59_13270"
     CDS             4013..4633
                     /locus_tag="VGA59_13270"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX9720690.1"
                     /translation="MSMEMTGEYRIPAPRQRVWEALNDPAILKACIPGCRQLEKVSDT
                     DFTAIVATKVGPVSATFRGSVNLSDLDPPNGYTLTGQGQGGAAGFARMGARVSLKQDS
                     DDTLLAYAAQADIGGKLASVGSRLVQTVARKNADDFFSAFARHLGGTGAQEPGLATTP
                     AGVAAVPAAGPAAGGLGLGAPVPAWVVVFGTALGLALGYCVALLAR"
     gene            complement(4698..5120)
                     /locus_tag="VGA59_13275"
     CDS             complement(4698..5120)
                     /locus_tag="VGA59_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9720691.1"
                     /translation="MIVLDTNVVSEPMRPNGNLAVRTWLDRQVAETLYLTATSLSELL
                     VGVEILPDGKRKDGLDTALSDLLDMLFGSRILPFDRTAAVKYAPLVSRARASGRPISV
                     ADGQIAAIAAVHGFTVATRDTAPFIAAGVPVTNPWEEA"
     gene            complement(5117..5356)
                     /locus_tag="VGA59_13280"
     CDS             complement(5117..5356)
                     /locus_tag="VGA59_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HEX9720692.1"
                     /translation="MAVVTVRNLPEETHRALKLRAAQHGRSTEAEIRAILEEQVRPKA
                     RVKIGSELAAFGERFGGIDLNIARDQTPTEPAEFE"
     gene            complement(5553..6575)
                     /locus_tag="VGA59_13285"
     CDS             complement(5553..6575)
                     /locus_tag="VGA59_13285"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX9720693.1"
                     /translation="MNIDELKSVANAIVAKPKGVLAADESNPTIKKRFDSIQVESTQE
                     NRRRFREILFTADGIERNIGGVILFDETLRQSARDGVPFPTLLAGRGIIPGIKVDQGA
                     KALALHPGNKVTEGLDGLRERLAEYRQLGARFAKWRAVIEIDERDIPSPFGIRANAHA
                     LARYAALCQEAALVPIVEPEVLMDGAHGIERCEAVTAQVLQAVFAELDAHHVLFEGML
                     LKPNMVIAGKKCARQAGVQEVAEATLRCLRRCVPPAIPGIVFLSGGQSAQDATDHLNA
                     MNAMGAQPWQVSFSYGRALQAPVLDAWQGQEGNVAAAQQALLKRCHLNGLAREGKYSL
                     SMEAAT"
     gene            complement(6627..7142)
                     /locus_tag="VGA59_13290"
     CDS             complement(6627..7142)
                     /locus_tag="VGA59_13290"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX9720694.1"
                     /translation="MKQILNVTAGFVAGAIAMYYLDSTMGRQRRALVRDKIAGVSHDA
                     ADFVEAKGKRMVDRAKGVLATGSIDRESSAEPQSDAQLRERVRSRMGHLVSHPRAIDV
                     SVDDGVVRLSGQILAKELDGLLSQVTEIAGVRKVHNALATLNDPSEFGSEQRGQEAAE
                     MGQSGSQTPRL"
     gene            complement(7223..8203)
                     /locus_tag="VGA59_13295"
     CDS             complement(7223..8203)
                     /locus_tag="VGA59_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX9720695.1"
                     /translation="MAPSSTRTLWKGAISFGLVYIPVTLHSATVEQRMKFNLLDKRTM
                     NPVGNLQVNKATGEPMPKEEIVKGYEYEKDQYVVVTPDEIKAALPKSTQTIEIESFVD
                     GGQIPSVYYDKPYYVAPAAKGQKPYLLLLETLRHTGKVGIARVVISTRQHLAALFPDE
                     QGLVLELLRWHDEVRDTAGLPLPGEDDAKLSARELKMAEQLVNELADKWKPEAFHDEF
                     REKLEKVVEEKVKAGQGEHIMKPLAGEEEAQPSADILDLTELLRRSLRKGAGAKREEE
                     EEEPARKSTARRKAANDEEAPARARRVAAASKSTARASTSVKPKSRKKAA"
     gene            8403..8636
                     /locus_tag="VGA59_13300"
     CDS             8403..8636
                     /locus_tag="VGA59_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720696.1"
                     /translation="MPVCDNCGNDYDKAFQVVKEGKTHTFDSFECAIARLAPVCEHCQ
                     VRIIGHGVESKGRYFCCSHCAEQHGVQGLTDRQ"
     gene            complement(8668..8838)
                     /locus_tag="VGA59_13305"
     CDS             complement(8668..8838)
                     /locus_tag="VGA59_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720697.1"
                     /translation="MGNKIVTEADRKRTPAPKTQEGIKVQAKGRGQKISLERGTSTRN
                     KKNPGKRPHQGG"
     gene            9025..9828
                     /locus_tag="VGA59_13310"
     CDS             9025..9828
                     /locus_tag="VGA59_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HEX9720698.1"
                     /translation="MQWKLKSALGAAALVLASQSMAQVTFYEGEGYRGRAFTTDRRVG
                     DFGRYGFNDRASSAVVERGRWEVCEHNRFEGRCVVLRRGSYDSLRAMGMENRISSVRP
                     LNDRRRYDNEAPEPVAAPGYEYRRRPSERTFEAPVTSVRAVVGPPNQRCWVERQQISQ
                     GGQPNVGGAVVGGLIGGILGHQVGKGTGRDVATVGGAVAGAAIGANTGGGSTGGPGYS
                     DVQRCENVASTTPAYWDVTYVFRGLEHRVQMSAPPGATILVNGNGEPRM"
     gene            complement(9869..10765)
                     /locus_tag="VGA59_13315"
     CDS             complement(9869..10765)
                     /locus_tag="VGA59_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9720699.1"
                     /translation="MARPLRVLKGTRILSLALNMPGPAALMRCRQMGATCVKLEPPSG
                     DPMGLYNKAAYAQLHEGVKILAADLKAPAGQKLLHRELAKADVLLTSFRPSALEKLGI
                     GWKKLHGLYPALSQVAIVGAPGARAEEPGHDLTYLADNDLVPGLELPATLYADMSGSL
                     MASEAVLQAVRPRNERYAGTGEADARGVFIEVALSEAAAYLALPRRWGLTQRSGPVGG
                     AHAGYRIYPGRDGRVAVAALEPHFAAALCAAAGAPASDLRAMFTPATHQAIAGFIATR
                     TREQLDRLAIERDIPLHTMTAT"
     gene            complement(10817..11992)
                     /gene="pobA"
                     /locus_tag="VGA59_13320"
     CDS             complement(10817..11992)
                     /gene="pobA"
                     /locus_tag="VGA59_13320"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEX9720700.1"
                     /translation="MRTQVAIIGAGPAGLLLGQLLFKAGIDNIILERQSGDHVLSRIR
                     AGVLEQVTVDLLDECGVGARMHREGLVHHGIELLFEGKRHRIDMQGLTGGKNVTVYGQ
                     TEVTHDLMDARKAQGLVTLYDTGDTATLHDFDGEQPKVRYVKEGKTHEIECDFIAGCD
                     GFHGVSRASAPANLLKTYEKVYPFGWLGLLADVPPVSHELIYANTERGFALCSMRSHT
                     RSRYYVQVPLQDKVEAWSDEAFWNELRSRLDPAARERLVTGPSIEKSIAPLRSFVAEP
                     MRFGRLFLAGDAAHIVPPTGAKGLNLAATDVKYLSNAFIEYYRDRSSHGIDHYSEVCL
                     RRIWKAERFSWWFTSLMHKFPETGEFGQKVQEAELDYLVNSRAASTSLAENYVGLPL"
     gene            12111..12869
                     /locus_tag="VGA59_13325"
     CDS             12111..12869
                     /locus_tag="VGA59_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX9720701.1"
                     /translation="MTIARADFIEGMAKGMAVLESFDTERQRLNATLAARRAGITRAS
                     ARRHLLTLAHLGYLETDGGYFWLAPKVLRFSGSYLASARLPRVLQPTLNRLAAQTAES
                     FSAVVLDGDQVVIVARSGSTRVLAYGLHLGARLPAHATSTGRVLLAAKRKTEFNGWMK
                     GRELARLTANTTVDPRKFRALIEQVRLQDFCVATEEHELGVQALAVPLRNMQGQAVSA
                     LNVVAAPHRLDARQMQRDMLPLLLDAARELRPLL"
     gene            12920..13120
                     /locus_tag="VGA59_13330"
                     /pseudo
     CDS             12920..13120
                     /locus_tag="VGA59_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901373.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
     gene            13178..13705
                     /locus_tag="VGA59_13335"
     CDS             13178..13705
                     /locus_tag="VGA59_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX9720702.1"
                     /translation="MLIDWFDTAPRRVLALVSAIGVGMLAYGLYLQHVVGLEPCPMCI
                     VQRYALVLVVLLAALTATSNNRGVLIAGSVLMLGAAGFGAFVAARQSWLQWYPPEVSS
                     CGRDFYGMIETFPLQRVIPMIFRGSGDCARVDWTFLGGSLANWSFVSFSVFTLVALVL
                     IVRLARRRALRPSHA"
     gene            complement(13694..15058)
                     /locus_tag="VGA59_13340"
     CDS             complement(13694..15058)
                     /locus_tag="VGA59_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX9720703.1"
                     /translation="MATHPTETIRTDSELITGLERHLHPLAGTPRDYDILLDLIGTAR
                     FALIGEASHGTREFYRERAEITKRLIIEKGFTAVAVEADWPDAWRVNRYVRGLSDDRD
                     APSSLAGFRRFPAWMWRNHEVRDFVEWLRDYNTGRGRETQVGFYGIDLYSLFTSIQAV
                     LAYLDRTDPEAARNARARYACFDHAREDSQAYGYSAGFGLAASCEDEVVQQLREMNRR
                     AAEVSQVSGLERDAAFFAQQNARLVRNAEEYYRTMFRGRVSSWNLRDSHMVETLQALD
                     RHLAADLGKPPRIAVWAHNSHLGDASATEMGNIGEWNVGQLVRDRHGDDAVLVGFSTH
                     RGWVTAAPDWDEPPQRMQVREGLEGSWEDIFHRTDMARALLVLRGNAELGALIATPRR
                     LQRAIGVIYRPETERQSHYFYTRLADQFDAVIHIDETHALEPLDQVAAWTTGEPPETY
                     PSGV"
     gene            complement(15080..15751)
                     /locus_tag="VGA59_13345"
     CDS             complement(15080..15751)
                     /locus_tag="VGA59_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX9720704.1"
                     /translation="MTDARLPFRDRHEAGRALADRLVHYHGRPNMLVLALPRGGVAVG
                     FEVARALHAPLDVIVVRKLGFPGHEEYAMGAIASGGVRVMNPMPGLRVSPDELAAVVA
                     REQDELVRRERLYRGDRPAVSMRGRNVIVVDDGLATGSTMRAAVLAIKQQHPAHLTVA
                     VPVGAPETCKALGAEADEVVCAAMPQPFRAVGLWYEDFPQATDEEVRDLLEEARREHA
                     TAAHG"
     gene            complement(15810..16496)
                     /locus_tag="VGA59_13350"
     CDS             complement(15810..16496)
                     /locus_tag="VGA59_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX9720705.1"
                     /translation="MNSPTHDFIEREVRIPSGDARLYGDLSLPPTFAGVVLFAHGSGS
                     GRHSARNRQVAQRLQQAGIATLLFDLLTAQEEQVDLHTREHRFDIPLLTRRMQDATTW
                     AWSQPELQHAPIGYFGASTGSAAALIAAARLGERIAAVVSRGGRPDLAGAAALAAVKA
                     PTLLIVGGADHGVIELNEEALQRLQCEKQLAIVPGATHLFEERGALEEAARMAASWFS
                     THMAHAEHPV"
     gene            complement(16748..>16998)
                     /locus_tag="VGA59_13355"
     CDS             complement(16748..>16998)
                     /locus_tag="VGA59_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9720706.1"
                     /translation="DGKLGRMGGAPPGPEGLNLDHFCLTIEPFEPEQLSAWLAGKGAT
                     ASEVARRYGAEGYGPSLYFSDPEGNLVELKGPSDGLPG"
BASE COUNT         2643 a         5592 c         5788 g         2975 t
ORIGIN      
        1 tcggtttcgc ccgagggcga cagccgtatc ccggcgcttt atcgcgagga actggcacag
       61 gagctggttg ccgacgcgct cgcacgagct cttgcccagt tgcggaaata gccagatgga
      121 caagcaaagc atcacgctca cggtgaatgg gcgcaatatc gagttcgagg cccagccgcg
      181 caagctgctg tcggatgccc tgcgcgaaga ttgccggctg accggcacgc acgtgggctg
      241 cgagcacggc gtttgcgggg cctgcactgt gctggtcgac ggccgtcccg tgcggtcctg
      301 cctgacctat gccatccaga tggaaggcca cgagctttca accatcgaat cggtggggca
      361 acccggggcg ctgtcgccgc tgcagcaggc cctgcacgag acgcacgggc tgcagtgcgg
      421 cttttgcacg cccggcatcg tcatgacgtt tcaggctttt ctagagggca acccggaccc
      481 gacggaggag cagatccgcg atgtgctgtc gggcaacctg tgccgctgca ccggctacca
      541 gaacatcgtg tcggccatcc agctggcggc gcggcgcatg cgcgaagggg caagtcatgg
      601 agcgaatggc gaggggcccc agcttgctgg ggatcgacac gcggaaggac ttggctgtgg
      661 cgacccccca accgttttgc aacatgacgg cataccggag ccacagcatg gcgcaagatg
      721 aattccgcca tatcggcgcc agccatccgc gcaaggagga cgagcggctg ctgaccgggc
      781 gcggccgtta catcgacgac atcgacgtgc cgggcgcgct gcatgcctgt ttcgtgcgtt
      841 cgccgcacgc gcatgcccgt atcgtgggga tcgactgtcg ggccgcgctg gacatgcccg
      901 gcgtggccgc ggtcgtgacc ggcaaggacc tggcggcctg gacgacgcgc catcgcatgg
      961 cgccggcgat cgagggcttg cagccggtgg agatggacac cttgccgatc gacaaggtcc
     1021 ggttccaggg cgacccggtc gcctgcgtga tcgcgcagga ccgttatgtg gccgaggacg
     1081 cggcggagca ggtggcggtg gactacgagg tgctgcccgc cgtgacgtcg atgtggcagg
     1141 cgctggatcc cgcatcgccg cgggtggacg agaccctgct gtccaacctg ctgtcgcatc
     1201 agcacgccgg acatggcgat gtgcaggcgc gcatgggcga ggcgcaccgc gtggtggaga
     1261 gcactttttc gcagcagcgc cagacccacc tgcccatcga gacccgcggc tgcatcgccg
     1321 tgtgggacga gggccgccgg cacctgacgt ttcatgtcgg cacgcaggtt ccgcatccgt
     1381 atcgcacgac gctcgccgcc cggctgcggc tttccgagtc gcaggtcacg gtgatctcgc
     1441 cggacgtggg cggaggattc ggccagaaga tcgcgctgta ccgcgaggaa ctgacggtcg
     1501 ccgccgtggc gcggcaactg aaagccccgg tgcgctggcg cgaagaccgg atggagaacc
     1561 tgctggcatc gtcgcaggcc cgcgaggatt tctgccgcac ccgcgccgcc gtcgacgcgc
     1621 agggccggtt gctggcgctg gacctggaga tcgtcgagga cttcggcgcc tatagcttct
     1681 acccgggcaa ctacctcgcc cgcgtggtgg cgatgatcct caccggcccc tacaaggtcc
     1741 gcgactacag ctatgacatc aaggtgctgc tgtcgaacaa ggtcggcaac ggtccgatgc
     1801 gcgctccgat gtccatcacg agctgggtga tggagggcac gatggacgcc atcgcgagca
     1861 acctgggcct cgacccggtg gccgtgcgcc gcatcaacat ggtggaggcg cgcgacctgc
     1921 cgtacacgat ggcgaccggc gaggtcctgg ccgacatcac gccgcgcgaa acgctggaag
     1981 ccgcgctggc cgcgatcgat taccgcgctt tccgggagcg ccagcgcgcc gagcgcgagc
     2041 acggcgtcta tctcgggttg ggcatctgca cggtggtcga gtcgaccacc tacggctcgc
     2101 gcttctacaa gtccgccggc atcccgggat cggggcacga agcggcctgg gtgcgcatcg
     2161 agccttccgg tgccgtgaat gcttcgtgcg gcctgggcgc gaccgggcag ggttacgaga
     2221 cttcgctggc caacgcggtg gcggaagggc tgggcgtgag cccggactgc gtcgagatcc
     2281 acctgggcca caccgatatc gctccgtacg gcatgggcag ccgcggcgca cgcggcggca
     2341 cggccggtgg cggcacgctt tatctttgcg cgctggacgc gcgggccaag gtccttgcga
     2401 tcgcggcaag gtgcctgggg ctggccggaa gcgccgggct tcgcatgtgc gagggccgga
     2461 tcgagcagca ggccgggtcg cagtggcgcg actgcgggtt gtcgctggcg gatatcgcga
     2521 ggaccgccta cctcgacccg ctggcgttgc cgccgggcat ggggccgggg ctcgaattcc
     2581 acaagaccta cgagccgccg ccaatgacct attcaaactc cacgcatgtg tgtgaagtgc
     2641 gcgtggaggt cgccaccggc gcgatccgct tcgagcgata cctggtggcg gaggactgcg
     2701 gcaccgtgct cagccctgtc gtcgtcgagg gccagcagca tggcgccata gcgatggggc
     2761 tgtccggggc gctgttcgag cacgtggaat acgacgacaa cgggcagaac ctttcgggct
     2821 cgctggccga ttacctggtg gcgaccgcgc acgagctgcc gcatatcgaa atcctgtcca
     2881 tgcacacccc gaacaaggcc acgccggcgg gcatcaaggg catggccgag ggcggcgtga
     2941 tgggggccat cggcgcgctc accaacgcgg tgaacgacgc gctggctccg ttcggtgtcg
     3001 tcgccagcca gcagccgctg accccgatgt acctgcgcgg gctgctgcgc ggccgccagc
     3061 cttccaccgt tttcactgcg ccggcgccag tcgacgcgca gcccgcaacc atcgagtaac
     3121 gaggcaagca aatgacatct accgacaaca aagcacgtgt cggcttcatc ggccttggca
     3181 tcatgggcca gagcatggcc ggccacatcc tcgcaggcgg ccatccgctg gccgtctaca
     3241 accgctcgcg cgacaaggcg gccgctttgc tggccagggg tgctcaatgg tgcgcaaccc
     3301 ccggcgaggt ggcaggccag tcggatgtgg tcatcaccat ggtgggctac cccagcgatg
     3361 tggaagaggt ctggctgggg ccggacggca tcctgtccca ggccaacgac gccctgctga
     3421 tcgacatgac cacttcaagc ccggaactgg cgcgccgcat cgccatcgcg gccgccggtc
     3481 gtcgctgcca ggcgctggac gcgcctgtgt ccggcggcga catcggcgcc cgcgacgcga
     3541 aactctcgat catggtggga ggcgccaggg aagcgttcga agacgccctg ccgatcctgc
     3601 aactgttggg caccaacgtc gtccaccagg gcgggccggg cgccggccag cacaccaaga
     3661 tgtgcaatca gatcgtgatc gcgtccacca tcatgggcgt gtgcgaaggg ctggcgtatg
     3721 gcaaggcggc ggggctcgac ctgcccaccg tgctcaagtc gatcggtgcc ggggcggccg
     3781 gtggcttcca gctcaacgtg ttcggggcca agatcatcaa cggcgacttc gccccgggat
     3841 tcttcatcga gcatttcgtg aaggacctgg gcatcgccct ggccgaagcg cagcggatga
     3901 agctggagct gccgggcctg gcgctggccc gcaagctgta catggtgctg gtcgagcagg
     3961 gtcacgcccg cctggggacg caggcactgt acaagtacta cgggacctcg gcatgagcat
     4021 ggagatgacc ggcgagtacc ggattcccgc gcccaggcag cgcgtctggg aagcgctgaa
     4081 cgacccggca attctcaagg cctgcattcc gggctgccgg cagctggaaa aggtttccga
     4141 taccgatttc accgcgatcg tggccaccaa ggtcgggccg gtgtcggcca ccttcagggg
     4201 gtccgtgaac ctgagcgacc tggacccgcc caacggctac acgctcaccg ggcaggggca
     4261 gggcggtgca gccggctttg cccgcatggg cgcgcgggtc tcgctcaagc aggacagcga
     4321 cgacactttg ctcgcgtacg cggcgcaggc cgacatcggc ggcaagttgg ccagcgtggg
     4381 aagccgcctg gtgcagaccg tggcgcgcaa gaacgcggat gattttttct cggcttttgc
     4441 acggcatctc ggtggcaccg gtgcgcaaga gccggggctg gccactacgc cggcaggcgt
     4501 ggccgccgtt cccgcagccg gaccagccgc cggagggctg ggtctgggcg ctcctgttcc
     4561 agcatgggtg gtcgtattcg ggaccgcgct tggcctggcc ctcgggtact gcgtcgcatt
     4621 gctcgcgcgt tagggtaatc ttggctggcc aggaactggt gcaagccagt gggagtgtgg
     4681 ccggcctggt ttgtgtgtta tgcctcttcc cacggattgg tgacaggcac gccggcggcg
     4741 atgaagggcg cggtatccct tgtcgctaca gtaaatccat gaacggccgc aatggcggca
     4801 atctgcccgt cagccactga gatcggtcga ccgctcgcgc gcgcgcggct gacaaggggc
     4861 gcatatttga cggcggccgt tcgatcgaac ggcaggatgc gcgatccaaa cagcatgtca
     4921 agcaagtcgc tcaaagcggt atcgagtccg tctttgcgtt tgccgtccgg caatatctcg
     4981 acgccgacca gcaactcgga caagctcgtc gccgtcagat acagcgtttc ggccacttgc
     5041 cgatccagcc aggtccgcac ggcaaggttg ccgttgggcc gcatcggctc cgaaacgaca
     5101 ttcgtgtcca ggacgatcac tcgaactcag ccggctcggt gggcgtctgg tcgcgggcga
     5161 tattcaggtc gatccccccg aagcgctcgc cgaaagcggc gagttccgag ccgatcttga
     5221 cccgggcttt gggccgcacc tgttcttcga ggatcgcccg gatttcggcc tcggtgctgc
     5281 gcccgtgctg agcagctcgc agcttcaacg cccgatgcgt ttcttcaggc agattgcgaa
     5341 cagtgacgac agccattgac ttcactaaaa ttatattgat gtcaatgata gcacgtctgc
     5401 tgaagcgaca tcaatggagc gccgggaagg ctgataaacg caatggaact caagcttacg
     5461 cgcgcgaact gcaacggact acctgagcag ctgggcccac catcgttggg cgcggcgtgc
     5521 acaaaaaaag cgctgcctaa atattcagca aatcatgtgg cggcttccat ggacagtgaa
     5581 tactttcctt cacgggccag gccgttgagg tggcaacgct tgagcagcgc ctgctgcgcg
     5641 gcggcgacgt tgccttcctg cccctgccac gcgtccagta ccggcgcctg caacgcccgc
     5701 ccatatgaaa aactgacctg ccacggctgc gcgcccattg cgttcatggc attcaggtgg
     5761 tcggtcgcgt cctgggcgct ctgcccgccg gacaggaaca caatccccgg gatcgccggc
     5821 ggcacgcagc gcctgaggca gcgcaacgtg gcctctgcca cctcctgcac gccggcctgg
     5881 cgcgcgcact tcttgccggc gatcaccatg ttcggcttca gcagcatgcc ctcgaacagc
     5941 acgtgatggg cgtcgagttc ggcgaacacc gcctgcagca cctgcgccgt caccgcctcg
     6001 cagcgctcga tgccgtgggc accatccatc agcacttccg gttccacgat cggcaccaga
     6061 gcggcctcct ggcacagcgc ggcgtaacgc gccagtgcgt gggcattggc gcggatgccg
     6121 aacggcgacg gaatgtcgcg ctcgtcgatc tcgatcaccg cccgccactt ggcgaaccgc
     6181 gcgcccagtt gccggtattc ggcgaggcgc tcgcgcaagc cgtccagccc ctcggtcacc
     6241 ttgttgccgg ggtgcagcgc cagcgccttg gcgccttggt cgaccttgat gccggggatg
     6301 attccgcggc ccgccagcag cgtggggaag gggacgccat cgcgcgcgct ctggcgcagg
     6361 gtttcgtcga acaggatgac gccgccgata ttgcgttcga tgccgtcggc ggtaaacagg
     6421 atttcgcgga agcgccggcg gttttcctgg gtggactcga cctggatcga atcgaagcgt
     6481 ttcttgatgg tggggttgct ttcgtccgct gccagcacgc ctttgggttt cgcgacgatg
     6541 gcattggcga cggacttgag ttcgtcgatg ttcacgggct gcgaccttcc ggtagaggtg
     6601 aagggatggc gacgatgttc attgccctaa aggcggggcg tctgcgatcc ggattggccc
     6661 atctccgcgg cttcctgccc gcgctgctcc gagccgaatt cgctggggtc gttcaacgtc
     6721 gccagggcgt tgtggacctt cctcacgccg gctatttcgg tcacctgcga cagcaaccca
     6781 tcgagttcct tggcaaggat ctgtccgctg agccgaacca cgccgtcgtc caccgagacg
     6841 tcgatggcac ggggatggct gaccaggtgg cccatccggg agcgcacccg ctcgcgcaat
     6901 tgcgcatcgc tttgcggttc ggcgctcgac tcgcgatcga tgctgccggt ggccaggaca
     6961 cccttggcgc ggtccaccat gcgttttccc ttggcttcca cgaaatcggc ggcgtcgtgg
     7021 gatacaccgg cgatcttgtc tcgcacgagc gcgcggcgct gccgccccat cgtgctgtcc
     7081 aggtaataca tcgcgatagc accggcgacg aatccggcgg tgacgttcag gatctgcttc
     7141 atccggggtt tccttccagc atgttcaatc cccgaatact agtcgcgccg cagctgggca
     7201 gtccgtaaga attctcgttt cttcaagccg ccttcttgcg cgatttgggc ttgacgctgg
     7261 tgctcgcccg ggccgtgctc ttggacgccg ccgccacacg gcgcgcgcgc gccggcgctt
     7321 cctcgtcgtt cgcggctttg cgcctggccg tggacttgcg tgccggctct tcttcctctt
     7381 cctcacgctt ggcgcccgct cccttgcgca ggctgcgccg cagcagctcg gtcaggtcga
     7441 ggatgtcggc gctcggctgg gcttcttcct cgcctgccag cggcttcatg atgtgctcgc
     7501 cctggcccgc cttcactttt tcttccacca ctttctccag cttttcgcga aactcgtcgt
     7561 ggaaggcttc gggcttccac ttgtccgcca gctcgttgac cagctgctcg gccatcttca
     7621 gttcccgcgc gctcagcttg gcatcgtctt cgccggggag gggcagcccc gcggtgtcgc
     7681 gtacctcgtc gtgccagcgc agcagctcca gcaccagccc ctgctcatcg ggaaaaagcg
     7741 ccgccaggtg ctgccgggtg gaaatcacca cgcgggcgat gccgactttg ccggtatggc
     7801 gcaaggtctc cagcaacaac aggtacggct tctggccctt ggccgcgggc gcgacgtaat
     7861 agggcttgtc gtagtagacg ctggggatct ggccgccgtc cacgaacgat tcgatctcga
     7921 tggtctgggt cgacttgggc aaagccgcct tgatctcgtc gggcgtgacc acgacgtact
     7981 ggtccttctc gtactcgtac cccttgacga tttcttcctt tggcatgggc tcgccggtgg
     8041 ccttgttcac ctgcaggttg cccaccgggt tcatggtgcg cttgtccagc aggttgaatt
     8101 tcatgcgttg ctcgaccgtg gccgaatgca gggtcacggg gatatagacc agcccgaagc
     8161 tgatcgcgcc tttccacagg gtgcgggtgc tggagggtgc catgatgctt caaccgtacg
     8221 cgttcgcgcg ctgtccggtg taggacgcgg cagcaaggtc ttgtgcgccg ctgccgttgg
     8281 gcagcgcgaa aggcgcgggc tcagccgcgt caaattccgg tcccgtccta cggaggcgca
     8341 acgccaggcg ggccgatagt tgcccggaag cggcgccgac gccggatcaa aggagcagcg
     8401 acatgcctgt ttgcgacaac tgcggtaacg attacgacaa ggccttccag gtcgtcaagg
     8461 agggcaagac gcacaccttc gacagcttcg aatgcgcgat cgcgcgcctg gcgccggtgt
     8521 gcgagcactg ccaggtgcgg atcattggcc acggcgtgga gtccaaaggc cgctatttct
     8581 gctgcagcca ctgcgccgag cagcacggcg tccagggcct gaccgaccgg cagtgacggg
     8641 cggccccggc cttgccttga aggccggtca gccgccttgg tggggccgtt tgccgggatt
     8701 cttcttgttg cgggtggacg tgccgcgctc caggctgatc ttctggccgc ggcctttggc
     8761 ctggaccttg atgccctcct gggtcttcgg tgcgggggtg cgcttgcggt cggcttcggt
     8821 gacgattttg ttgcccattg gatactccaa ctgaattgac ggatcgggcg attaggccac
     8881 agcctgcgac cggtccgccg tcgagggccc agcaaccacg catggacagg cacgggtgag
     8941 acgatgcgtt cgcttacacg gttgatggcc gcgctgaaat cggctgacct atcatgaacc
     9001 gtccgaactg aacgggagca cactatgcaa tggaagctga aatccgcgct gggcgcggcc
     9061 gcgttggtcc ttgcttcgca atcgatggcg caggtcactt tctacgaagg agagggctac
     9121 cgcggccgcg ctttcaccac cgaccggcgg gtcggcgact tcggccgtta cggcttcaac
     9181 gacagggctt cctcggcggt cgtggagcgc ggccgctggg aggtctgcga gcacaaccgc
     9241 ttcgaggggc gctgcgtggt actgcgccgc ggcagctatg actctctgcg cgccatgggc
     9301 atggagaacc gcatatcttc ggtgcggccg ctcaatgacc ggcggcgcta cgacaacgaa
     9361 gcgcccgagc ctgtggcggc gcccggctac gaataccggc gccgtcccag cgagcggacc
     9421 ttcgaggcgc ccgtgacctc ggtgcgcgcc gtggtcgggc cgcccaacca gcgctgctgg
     9481 gtggagcgcc agcagatcag ccagggcggg cagccgaacg ttggcggagc cgtcgtcggc
     9541 ggtttgatcg gcggcatcct gggtcaccag gtcggaaagg gcaccggacg ggacgtggca
     9601 actgtcggcg gcgcggtggc gggcgcggca atcggcgcga acacgggtgg cggcagcacc
     9661 ggtggccccg gctacagcga cgtgcaacgc tgtgaaaacg tcgccagcac gacgcccgcg
     9721 tattgggacg tgacctatgt cttccgcggg ttggaacatc gcgtccagat gagcgcgccg
     9781 ccgggagcga ccatcctcgt caacggcaac ggcgagccgc gcatgtagca gccgccgcga
     9841 gtcgcgttcg caatcggcgc cgtcgctgct acgtcgctgt catggtgtgc agcggtatgt
     9901 ccctctcgat ggccagccgg tccaactgct cgcgcgtgcg tgtggcgatg aaaccggcga
     9961 tcgcctggtg cgtggccggc gtgaacatcg cgcgtaggtc cgatgccggt gcgcccgccg
    10021 cggcgcacag ggcggccgcg aagtgcggct ccagcgcggc gacggccacc cggccgtctc
    10081 tgcccggata aatgcgatag ccggcgtgcg cgccgccaac cgggccgctg cgttgcgtca
    10141 gcccccatcg ccgtggcagc gccaggtacg ccgccgcctc cgacagcgcc acttcgatga
    10201 acacgccgcg cgcgtcggcc tcgccggtgc ccgcgtagcg ttcgttgcgc ggccgcacag
    10261 cctggagcac agcctcgctc gccatcaagg atccgctcat gtcggcgtag agcgtggcgg
    10321 gcagttccag cccgggcacc agatcgttgt cggcgagata cgtgaggtcg tggcccggtt
    10381 cctcggcgcg cgcgccgggc gcgccgacga tcgccacctg cgacagcgca gggtagaggc
    10441 catgcaattt cttccagccg atgccgagct tttcgagggc cgacggcctg aaggacgtga
    10501 gcagcacatc ggcctttgca agttcgcggt gcagcagctt ctggccggcc ggcgccttca
    10561 ggtcggcagc caggatcttc acgccttcat gcagttgcgc gtaggcggcc ttgttgtaca
    10621 ggcccatcgg gtcgccggac ggcggctcca gcttgacgca ggtcgcgccc atttgccggc
    10681 agcgcatcag cgcggctggg cccggcatgt tcagtgccag gctgaggatg cgagtacctt
    10741 tgagaacacg gaggggacgg gccatggtcg ctatggaaga gctctgtttg tgcaagacta
    10801 gcagatcgac ctgggctcat agcggcaggc ccacgtagtt ttccgccagc gacgtggacg
    10861 ccgcgcgtga attgaccagg taatccagct ccgcctcctg caccttctga ccgaactcgc
    10921 ccgtctccgg gaatttgtgc atgagcgagg tgaaccacca ggagaagcgt tccgccttcc
    10981 agatgcggcg caggcacact tcggagtaat gatcgatgcc gtggctgctt cggtcacggt
    11041 aatactcgat gaaggcgttc gacaggtact tcacgtcggt ggccgccagg ttcaggccct
    11101 tggcgcccgt gggcggcacg atgtgcgcgg cgtcgccggc caggaacagc cggccgaagc
    11161 gcatgggctc ggccacgaag ctgcgcaacg gggcgatgct tttttcgatc gacggcccgg
    11221 tgacgagccg ctcgcgcgcc gccggatcga gccgggaccg caattcattc cagaacgcct
    11281 cgtccgacca tgcctcgacc ttgtcctgca gcggcacctg cacgtaatag cggctgcggg
    11341 tgtggctgcg catcgagcac agggcgaaac cgcgttcggt gttggcgtag atgagttcat
    11401 gggacaccgg cggcacgtcc gccagcaggc ccagccagcc aaaggggtag accttttcgt
    11461 aggtcttcag aagattggcc ggggcgctcg cgcggctcac gccgtggaag ccgtcgcagc
    11521 cggcgatgaa gtcgcattcg atctcatgcg tcttgccttc cttcacgtag cgcaccttgg
    11581 gctgctcgcc gtcgaagtcg tgaagcgtgg ccgtgtcacc ggtatcgtag agggtgacca
    11641 ggccctgcgc cttgcgcgca tccatgaggt cgtgcgtcac ctcggtttgg ccatacaccg
    11701 tcacattctt gcccccggtc aggccctgca tgtcgatgcg gtggcgcttg ccctcgaaca
    11761 gcagctcgat cccgtggtgc acgaggccct cgcggtgcat gcgcgcgccg acgccgcatt
    11821 cgtccagcag gtccaccgtg acctgctcca gcacaccggc acggatgcgg ctgagcacgt
    11881 ggtcgccgct ctgccgctcg aggatgatgt tgtctatgcc ggccttgaac agcaattggc
    11941 cgagcagcaa gcccgcgggg ccggcgccga tgatcgcgac ctgggtgcgc atggaagtct
    12001 cctgtttgtc tcgcgagctt aggacgcact agggttttcc ggtaggggcg cgaagcgatg
    12061 ctgtgcgatc attgccggca ctgcgcgatg atcgcgcaag ggaccgaccc atgaccatcg
    12121 ccagggcgga tttcatcgaa ggcatggcca agggcatggc tgtgctggag agcttcgaca
    12181 ccgagcgcca gcggctgaac gccacgctgg cggcgcggcg cgccggcatt acacgcgcgt
    12241 cggcgcggcg ccacctgctc accctcgctc acctggggta cctggaaacc gacggcggct
    12301 atttctggct ggcgcccaag gtgctgcgct tttcgggaag ctacctggct tccgcgcggc
    12361 tgccgcgggt gctgcagccc acgctcaacc gcctggcggc ccaaacggcc gagtcttttt
    12421 ccgcggtggt gctcgacggc gaccaggtcg tgatcgtcgc gcgcagcggg tccacccggg
    12481 tgctggcgta tggcctgcac ctgggcgcgc gcctgccggc gcatgccacc tccaccggcc
    12541 gtgtgctgct ggcggccaag cgcaagaccg agttcaacgg ctggatgaag ggcagggaac
    12601 tcgcgcgcct gaccgcaaac acgacagtgg acccgcgcaa gttccgggcg ctgatcgagc
    12661 aggtacgcct ccaggacttc tgcgtcgcga ccgaagaaca cgaactgggc gtgcaggctc
    12721 tcgctgtgcc gctgcgcaat atgcagggac aggcggtgtc ggccttgaac gtggtggccg
    12781 ccccgcatcg gctcgacgcc cggcagatgc aacgcgacat gctgcccctg ctgctggacg
    12841 ccgccaggga actgcgtccc ttgctgtgac gccttgttca tcagccacgg cgaggggctg
    12901 gaccccgccg ggcgcccccc gggccgttgt ggtcgggccg atcgcccggc tggaacttat
    12961 tccggccggc gacaccaaac ccgcaggaga taatgagaaa gattccatca ttgaggcgca
    13021 gatatcggcg cagcaattca gggatctggg ctttcgtgag ggcgagacgc tggtggtgac
    13081 gccgcgccgg gcccgcgtgt tcgtggaagg cggcgcctga gcggcagcgt tcggcggcgg
    13141 gtgcagggcc ccggccttgc cgttttggag gaagagaatc ttgatcgact ggtttgatac
    13201 cgcgcctcgc cgcgtgctcg cgctcgtgag cgccatcggc gtgggcatgc tggcctacgg
    13261 cctttacctg cagcatgtgg tggggctgga gccgtgcccg atgtgcatcg tgcagcgcta
    13321 cgctctggtg ctggtggtgc tgctggcggc gctcacggcg acctcgaaca accgcggcgt
    13381 gctgattgcc ggcagcgtgt tgatgctggg ggcggcgggt ttcggcgctt tcgtcgccgc
    13441 gcgccagagc tggctgcagt ggtatccgcc cgaggtctcg tcctgcgggc gcgacttcta
    13501 cggaatgatc gagacctttc ccctgcagcg cgtcattccc atgatcttca ggggcagcgg
    13561 cgactgcgcc agggtcgact ggaccttcct gggcggctcg ctagccaact ggtccttcgt
    13621 gtcattcagc gtcttcacgc tggtggcgct agtgctgata gtgcgtctgg cccggcgccg
    13681 cgccctgcgg ccgtcacacg cctgagggat aggtctccgg cggctctccg gtggtccagg
    13741 ccgccacttg gtccagcggc tcaagcgcgt gggtctcgtc gatgtggatc accgcgtcga
    13801 actgatcggc cagccgggtg tagaaatagt ggctctggcg ctcggtctcg ggccggtaga
    13861 tcacaccgat ggcgcgctga agccggcgcg gggtggcgat cagcgcgccc agttcggcgt
    13921 tgccgcgcag caccagcagc gcccgggcca tgtcggtgcg gtggaagatg tcttcccagc
    13981 tgccctccag gccttcgcgg acctgcatgc gctgcggcgg ctcgtcccaa tccggcgccg
    14041 ccgtgaccca tccgcggtgc gtgctgaacc ccaccagcac cgcatcgtcg ccatggcggt
    14101 cgcgcaccag ctggcccacg ttccattcgc cgatgttgcc catttcggtg gccgaggcgt
    14161 cgcccaggtg cgagttgtgg gcccacacgg caatgcgggg cggtttcccg agatcggccg
    14221 ccagatggcg gtccagcgcc tgcagggtct ccaccatgtg gctgtcgcgc agattccacg
    14281 acgacacccg gccgcggaac atggtgcggt agtactcctc ggcgttgcgc accaggcgcg
    14341 cgttctgctg ggcaaagaaa gcggcgtcgc gctccaggcc ggacacctgc gacacctccg
    14401 cggcgcggcg gttcatttcg cgcaactgct gcaccacctc gtcctcgcag ctggccgcga
    14461 gtccgaagcc ggcggagtaa ccgtaggcct ggctgtcttc ccgcgcgtgg tcgaaacagg
    14521 cataacgcgc acgggcgttg cgcgccgctt cggggtccgt gcggtcgagg taggccagca
    14581 ccgcctggat cgacgtgaac aggctgtaca gatcgatgcc gtagaacccc acctgcgttt
    14641 cccgcccgcg gccggtgttg tagtcgcgca gccattccac gaaatcgcgc acttcgtggt
    14701 tgcgccacat ccaggcggga aaacgccgga atcccgccag ggacgagggc gcgtcgcggt
    14761 cgtcggacaa gccgcgcacg taacggttga cacgccaggc gtcgggccag tcggcttcca
    14821 cggccacggc ggtgaagccc ttctcgatga tcagtcgttt ggtgatttcg gcgcgttcgc
    14881 ggtagaactc gcgcgtgcca tgcgaggctt caccgatcag cgcaaagcgg gccgtgccga
    14941 tcaggtccag cagtatgtcg tagtcgcgtg gggtgccggc aagggggtgc aggtgccgct
    15001 ccaggccggt gatcagttcg ctgtcggtgc ggatggtttc tgtcgggtgg gtggccatgt
    15061 attcctgctc ctcggacagt catccgtgcg ccgccgtcgc atgctcgcgt cgtgcctctt
    15121 caagcaggtc gcgcacctct tcgtcagtcg cctgcgggaa atcctcgtac cacaggccca
    15181 cggcgcggaa cggttgcggc atcgcggcgc agacgacttc gtccgcctcg gcgcccaggg
    15241 ccttgcaggt ctccggggcg ccgaccggca cggcgacggt caggtgcgcc ggatgctgct
    15301 gcttgatggc cagcacggcg gcacgcatgg tcgagccggt tgccagcccg tcgtccacca
    15361 cgatcacgtt gcgtccgcgc atgctcacgg ccggccggtc gccccggtaa agccgctcgc
    15421 ggcggaccag ttcgtcctgt tcacgggcca ccacggccgc cagttcatcc ggcgataccc
    15481 tgaggccggg catcggattc atgacgcgca cgccaccgct ggcgatcgcg cccatcgcat
    15541 attcctcatg gcccggaaag ccgagcttgc gcaccacgat gacgtccagc ggcgcgtgca
    15601 gcgcgcgcgc cacttcgaac cccacggcga cgccgccgcg cggcaaggcc agcaccagca
    15661 tgttgggacg cccgtggtaa tgcaccagcc ggtcggccag agcccgcccg gcttcgtgcc
    15721 ggtcacggaa cggcaatctt gcatcggtca tcgtcgggcc tcacgttggc cgccgaggcg
    15781 tctgcattgc cggcggttct gcgctgcgct cataccggat gctccgcatg cgccatatgg
    15841 gtgctgaacc aggaagccgc catccgcgct gcctcttcca gggcgccgcg ctcctcgaac
    15901 aggtgcgtgg cgccgggcac gatggcgagc tgcttttcgc actgcagccg ctggagggct
    15961 tcctcgttga gttcgatcac gccatggtcg gcaccgccca cgatcagcag ggtgggagcc
    16021 ttgaccgcgg ccagcgccgc ggcgccggcc aggtcggggc gcccgccgcg cgacaccacg
    16081 gccgctatgc gctcgcccag ccgcgcggcg gcaatgagcg cggcggcgct gccggtgctc
    16141 gcgccgaagt aaccgatagg cgcgtgctgc agttcgggct gcgaccaggc ccaggtggtg
    16201 gcatcctgca tccgccgcgt cagcagcggg atgtcgaacc ggtgctcgcg ggtatgcagg
    16261 tcgacctgct cttcctgcgc cgtgagcagg tcgaacagca gggtggcgat cccggcttgc
    16321 tgcagccgct gcgcgacttg ccggttgcgc gcgctgtggc ggccgctgcc gctgccgtgc
    16381 gcgaacagca cgaccccggc aaaggtgggc ggcagggaca ggtcgccata gagccgtgcg
    16441 tccccggacg ggatgcgcac ttcgcgttcg atgaaatcat gagtggggct gttcatctcg
    16501 gtcaccagtt ccttccaagc taggcgttgc ggctcgcctt ggctgtcgga tcacttccat
    16561 ttccatgtag gaaacgtgcc gtccggacgg tttcgacggc ccagccgcgc gctgtcgtgg
    16621 cacggtcctg gggccctttg ttgctcaaac cctacgctag gccgaaggcc gaggcgattt
    16681 gttgaccccg gtcaaaaaac cgggcgccaa cgcgtcaagg ctccggcgtt gcgcaagccg
    16741 cgttcggtca gccggggagc ccgtcggaag ggcctttgag ctccaccagg ttgccttcgg
    16801 gatcgctgaa gtacagcgac gggccatagc cttcggcgcc gtagcggcga gcgacttcgc
    16861 tagccgtcgc gcccttgccg gccagccagg cggacagttg ctccggttcg aacggctcga
    16921 tggtcaggca gaaatggtcg aggttgagcc cttcgggacc gggcggagcg ccacccatac
    16981 ggccgagctt gccgtcca
//
LOCUS       DASWIY010000372        12565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_625319, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000372 DASWIY010000000
VERSION     DASWIY010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 12565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12565
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625319"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VGA59_13360"
     CDS             complement(<1..832)
                     /locus_tag="VGA59_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9720707.1"
                     /translation="MKLATYRDGSRDGQLVVVSRDLGTAHYATGIADRLQQVLDDWSF
                     LSPQLQDLYDTLNNGKARHAFPFDPGQCMAPLPRAYQWADGSAYINHVELVRLARHAE
                     VPANYYTEPLMYQGGSDDFLGPQDDVVVASGEYGIDFEAEVAVVTGDVAMTATPEQAL
                     EGIRLVMLANDVSLRNLIPDELAKGFGFFQSKPATAFSPVAVTLDELGEAWDKGRLHL
                     TLQSTWNGRTVGMCDAGKEMTFHFGQLIAHVCKTRNVRAGSIIGSGTVSNRATEADGR
                     K"
     gene            932..1708
                     /locus_tag="VGA59_13365"
     CDS             932..1708
                     /locus_tag="VGA59_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9720708.1"
                     /translation="MKERAGIQSVEVGFALLEVLSQAPGPLMLRDLAAAAGMSAAKAH
                     RYLVSFQRLQLVAQDAGSTRYDLGPAALKLGLASLSRLDAVRMARERVTALMEQIGHT
                     LALAVWGNHGPTIVHWEESPQAVTVNLRLGDVMPLLSSATGRCFAAYLSQDAIAPLLK
                     DEIARAQKLRRTDVPVTLAEARALLGEVRRRGAARVVDTLLPGIVGFCAPVFDSDGHI
                     ALGMVALGPLGSFDPEWNGAIDKPLRAAASQLSSDLGYRG"
     gene            1705..2724
                     /locus_tag="VGA59_13370"
     CDS             1705..2724
                     /locus_tag="VGA59_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX9720709.1"
                     /translation="MKPASAIPLRPLLALSAAVLAAHLLLLRAAPGPLHAPPKAGAQP
                     FITRSITAAPRPAAAAVVPPAVPRPSRPALARHGPVAASTVQGALTTAPDHAAPEAAS
                     PAAPMPLPPATAAPPGPIQAAPAMRFTVPGSMRLRYTVAAQVRGQARAGESELVWRHD
                     GESYEARLEVDAAPLPKRTQRSTGRITPDGLAPLRFSEKLRNEQAAHFERDAGKVSFS
                     SNRPDAALLAGAQDRLSVLLQLAAMLAGQPEKFPPSATVIVQTAGTRDAEQWLFTVEQ
                     EESLHLPGGTVSALKLTRNPRKEFDQKLEIWLAPGMDYVPVRLRLTQPNGDSLDQLWS
                     STDKG"
     gene            2834..3106
                     /locus_tag="VGA59_13375"
     CDS             2834..3106
                     /locus_tag="VGA59_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HEX9720710.1"
                     /translation="MQMLYDSDSFVVVHMQANEPLDGEPPPRIVRHGFEIVDKRSNKE
                     VYLDGSWAEAFQRQINAWQQNTPTQEEVEETLDGYAELAQNPLVMH"
     gene            3355..4188
                     /locus_tag="VGA59_13380"
     CDS             3355..4188
                     /locus_tag="VGA59_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX9720711.1"
                     /translation="MNRCLASALLVCLVSAACAVPAVSEEPAARLHQLLPADAILLGE
                     QHDAPDHQRIHREVIEALAARGALAAVALEMAPQGGSTAGLARDATESAVQAALRWTD
                     ASWPWAVYAPAVMAAVRSGVPVLGANLPRAAMRLAMADSQLDSLLPGPALKAQQQAIR
                     QGHCDMLPETQIAPMTRVQIARDRAMAQTLVASASPGKSVVLLAGGRHVDRRVGVPLH
                     LPPGLQTRVVALRAGAPPDDSRRVADSDAIWTTPPVPPKDYCADMRPRSSAQPAGAEK
                     P"
     gene            complement(4228..4956)
                     /locus_tag="VGA59_13385"
     CDS             complement(4228..4956)
                     /locus_tag="VGA59_13385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX9720712.1"
                     /translation="MPDPQEGPAGSGQAPADSDQPAVSRPDVARVGVIVPTYNRPDLL
                     RSCVLQLAAQSRPPDIICVHQNGHPDSYAWAVADLQTASRIAWLHTPGKIPQHQWYAV
                     PLKFLLESGCTHFFWADHDDLYLREHIASGLRDLEQFDFSVSSRCGLLFTKGSDFRYA
                     HEVDFTVHAPGGMSSTMCFNRQFAQELLSDIARDGQNYYTDNVVARVTMPKFRCRVSN
                     RRTSIYHAHEGSITSNQWLAKAFA"
     gene            complement(4949..6283)
                     /locus_tag="VGA59_13390"
     CDS             complement(4949..6283)
                     /locus_tag="VGA59_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9720713.1"
                     /translation="MAYNDFSMDNHWLPFTPNRQFRKDPRIFVAADGMHFTTHDGKQV
                     IDGISSLWCVGAGHNRKPINDAIKKQLDTLDYATAFQASNDRAFQAAEKIAAMAPGDL
                     NKVLFCNSGSEAADTSLKVALAYHRARGEGHRNVFIGREKGYHGVGFGGMSVGGIPAN
                     RKVYGAAFLPRVDHMRFIHDPVNNAYIHNREPVWQEDILLELENRILPLHDPSNVAAI
                     MVEPVAGSAGWYLPPQGYLKRLREICDKHGILLIFDEVITGFGRMGTGFASDYYGVVP
                     DMLNFAKCVTNGVIPMGGVICRDKLYDAMMKTEAPEHVVEFFHGYTYSGHPVACAAAI
                     ATLDLYKQENLFQRAGEMGKVLGDAFHATFKGLPNVIGIRSLGLAAAVELAPIAGSPG
                     KRAFDVYIDCFHKGALVRPAGDVLVIAPPYIVEKEHIDTLVNTLADSIRQHA"
     gene            6377..7225
                     /gene="purU"
                     /locus_tag="VGA59_13395"
     CDS             6377..7225
                     /gene="purU"
                     /locus_tag="VGA59_13395"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX9720714.1"
                     /translation="MNRTYILTLSCPDRPGIVHAVSGFLLERGGNIEEAAQYNDHDTG
                     LFFMRVQFACDQLTFEDLKVQLKFFADPFKMTWKLHARSEPMRTVILVSKEGHCLNDL
                     LFRWKSGLLPLEIAAIVSNHRDFYQLAASYNVPFHHMPVAAATKAQVEARQFEIIEAE
                     GAELVVLARYMQILSDELCRKLAGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATA
                     HYVTADLDEGPIIEQDVARVDHSATVEDLTAQGRDTESQVLARAVKWHSEHRVLINGH
                     KTVIFK"
     gene            7406..8272
                     /locus_tag="VGA59_13400"
     CDS             7406..8272
                     /locus_tag="VGA59_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9720715.1"
                     /translation="MTARIPPIQCLLTFEALARLRSVTLAADELCVTPSAVSHRVKQL
                     EQIIGTKLFGRADFSLTTEGNEYLAHVREGLSILERFPGKDSAQGKRKLRVAVTPTFS
                     RSILMPRLRGFIEAYPEIDLTLQVSIPLLDVVAEDADLMIRYGTGRYADVEHLCLMTD
                     EVTPLASPMYIRENGPFDTIEELLAAKLIKSPLEPWRTWFLAHGVDAGEPGDGSSFND
                     IGLVCDAAAQGLGIALVRLKLGQPWLDNGSLARISERNVPSPHAHYLCWRTGTMDRWE
                     CAAFADWLKKSV"
     gene            complement(8358..9146)
                     /locus_tag="VGA59_13405"
     CDS             complement(8358..9146)
                     /locus_tag="VGA59_13405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9720716.1"
                     /translation="MSTLMSNAIFGLVFVLAATGLFFFVVTRGALIVRGLAGAGHESS
                     HVYPIYSNMRLIKMVDYQPIISAILLFQYPRLVSKIVAGLRATDLRNKKVLVTSCAFG
                     NVIPRVVDASLQSGAERILIADIIKNELVHAKSKLSHYGAKIQYVEDNATSMRQEEGI
                     ADANVMFFLLHELPHELKAKALSEAGRMLKPGGKLYLGEFHKPELWVLRALSWTYFKV
                     FEPLGLALWDTHDPLLLLQEMGGWTVERTTFFFGNFQIITATKH"
     gene            9435..10928
                     /locus_tag="VGA59_13410"
     CDS             9435..10928
                     /locus_tag="VGA59_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9720717.1"
                     /translation="MNAAPEPSLLGRAERQSQVVGALQAVLPPDALLWHAEDTTPYEC
                     DGLTAYRQRPLVVALPESETQVQAVLRTCHALDVPVVARGAGTGLSGGAMPHALGVTL
                     SLARFNRILKLDPAARTALVQCGVRNLAISEAAAAYGLYYAPDPSSQIACTIGGNVAE
                     NSGGVHCLKYGLTLHNVIKVRGFTVQGEPVDFGSDALDCAGFDLLSAIVGSEGMLAVV
                     TEVTVKLVPSPLLARCIMASFDDVRKAGDAVAAVIAAGIIPAGLEMMDKPMTAAVEDF
                     VHAGYDLTAQAILLCESDGTPEEVEEEIARMSEVLRSHGANAIAVSRDEAERLKFWSG
                     RKNAFPASGRLSPDYMCMDSTIPRKRLADILLAIQQMEKKYALRCANVFHAGDGNLHP
                     LILFDANDADQLHRAELFGAEILETSVAMGGTVTGEHGVGVEKLNSMCVQFSTAECEQ
                     MFGVKRAFDAKGLLNPGKVVPTLNRCAEYGKMLVRGGRLAHPDLPRF"
     gene            complement(10933..>12565)
                     /locus_tag="VGA59_13415"
     CDS             complement(10933..>12565)
                     /locus_tag="VGA59_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720718.1"
                     /translation="DAGTGSNSLTAAGDILFNGATLSGTGTSTLSAAAAQVAGSTTLN
                     TSLSGTPLDIQGTLAIGADVSVSSLNLAGAGTITGAGNLTVGGSLTWSGGSMTGSGST
                     TLGPSASASITGPVLLARPLATASSGIIKMSGTGQIALDSGASLTNAGTFDIQNNGGF
                     TPAPSPSPVPIVNSGVLKKSAGTGLSLLDGVSFSNTGTLAVSNGTLQFGSAAFSSNSG
                     LILLASGATLDNNGTTLANTSTGVISGSGTLKLGTGTFNNAGQLRPGGLGSMGTLTLD
                     AATVNLDSGTSLYLDIGNRSVPGSTYDVLSITGTPGDVNLASSDVTVNANLISPSIAV
                     GDTFDIVSATGSVAGGALPAVAGFALSVVAGPPQAIRATATVPVPAPTASPTPQPTPE
                     PTPAPTPEPTPEPTPAPTPAPTPAPTPAPTPEPTPAPTPAPTPAPTPAPTPEPTPAPT
                     PAPTPEPTPAPTPARTPDPTPAPRPAPTGASTVDRIIDLFPSEDRGDVMAALADLETN
                     TLANFVTLLIGEEQKQARQRRQGTDNLVDDNQCLR"
BASE COUNT         2067 a         4132 c         4231 g         2135 t
ORIGIN      
        1 ccttccggcc atcggcctcg gtggcgcggt tgctcacggt gcccgagccg ataatgctgc
       61 cggcgcgcac attgcgggtc ttgcagacgt gggcgatcag ctgcccgaag tggaaggtca
      121 tctccttgcc ggcgtcgcac atgcccacgg tacgcccgtt ccaggtgctt tgcagcgtga
      181 ggtgcagccg gcccttgtcc caggcctcac ccagttcgtc cagcgtgacg gccaccgggc
      241 tgaaggctgt cgcaggcttg ctctggaaaa agccgaaacc tttggccagt tcgtcgggga
      301 tcaggttgcg taacgacacg tcgttggcca gcatcaccag gcggatgccc tccagcgcct
      361 gctcgggcgt cgcggtcatg gcgacgtcgc cggtgaccac ggcgacttcg gcttcgaagt
      421 cgatgccgta ctccccgctg gccacgacca catcgtcctg cgggcccagg aagtcgtcgc
      481 tgccgccttg gtacatcagc ggctcggtgt agtagttggc cggcacctcg gcgtgccgcg
      541 ccagccgcac cagttccacg tggttgatgt aggccgagcc gtccgcccac tgataggcgc
      601 gcggcagcgg cgccatgcat tgccccgggt cgaacgggaa agcgtggcgg gccttgccgt
      661 tgttcagagt gtcgtacagg tcctgcaatt gcggcgacag gaagctccag tcgtccagca
      721 cctgctggag gcggtcggcg atgccggtcg cataatgcgc cgtgccgagg tcgcgtgaga
      781 ccacgaccag ctggccgtcg cgcgagccgt ccctgtacgt agcgagcttc attcggaatt
      841 gcctgtgcgg gttgaatgcg ctagagtcgc aagacgttac gcattgtgaa atgaatttac
      901 ctaatcgtaa ttctacagga agcggctccg catgaaggag cgcgccggca tccaatcggt
      961 cgaggtcggc ttcgctttgc tggaggttct gtcgcaggcg cccggcccgc tgatgctgcg
     1021 cgacctggcc gccgcggccg gcatgagcgc ggccaaggcg caccgctatc tggtgagttt
     1081 ccagcggctg caactggtgg cgcaggacgc cggcagcacg cgctacgacc tgggaccggc
     1141 tgcgctcaag ctggggctgg cttctctatc acggctggac gccgtcagga tggcccgcga
     1201 gcgcgtcacc gcattgatgg agcagatcgg ccacaccctg gcgctggccg tgtggggcaa
     1261 ccatgggccc accatcgtgc attgggagga atcgccgcag gcggtcaccg tcaacctgcg
     1321 gctgggcgat gtgatgccgc tgctgtcttc ggccaccggc cgctgctttg cggcctacct
     1381 gtcgcaggac gcgatcgccc ccctgctcaa ggatgaaatc gccagggcac agaaactgcg
     1441 ccgcaccgat gtgccggtca cgctggccga agcccgcgcg ctgctgggcg aggtgcgccg
     1501 tcggggcgcc gcgcgggtcg tggacacgct gttgccgggc atcgtgggtt tttgcgcgcc
     1561 ggtgttcgat tcggacgggc atatcgcgct cggaatggtc gcgctcgggc cgctgggcag
     1621 cttcgacccc gaatggaacg gcgcgatcga caaaccgttg cgcgccgccg cgagccagct
     1681 gtccagcgac ctggggtacc gggggtgaaa ccggcatcgg ccattccgtt gcggccgttg
     1741 ctggcgcttt ccgcggccgt gctggcggcc catctgttgc tgctgcgcgc ggcgccgggt
     1801 ccgttgcatg ccccacccaa agcgggcgcg cagccgttca tcactcgcag catcaccgcc
     1861 gcaccgcgcc cggctgccgc ggcggtggtc ccgccggccg tgccgaggcc ttcgcggccc
     1921 gccctggcgc ggcatggccc cgtcgccgcg agcacggtgc agggcgctct cacgacagcg
     1981 cctgaccatg ccgcgccgga ggccgcgtca ccggcggcgc caatgccttt accgcccgcg
     2041 acggccgcgc cgcccggccc gattcaggcg gcgcccgcca tgcggttcac ggttcccggc
     2101 tcgatgcggc tgcgctacac ggtcgcggcc caggttcgcg gccaagcccg ggccggcgag
     2161 agcgagctgg tctggcgcca cgatggcgaa agctacgagg ccaggctcga agtcgacgcg
     2221 gcgccgctgc caaagcggac gcagcgcagc acgggccgca tcacgccgga cgggctggca
     2281 cccctgcgtt tttccgaaaa gctgcgcaac gagcaggccg cgcatttcga gcgcgatgcc
     2341 ggcaaggtga gcttcagcag caacaggccc gacgccgcgc tgctcgccgg cgcccaggac
     2401 cgcctgagcg tgctgcttca actggcggcg atgctggccg gccagccgga gaaattccca
     2461 ccgtccgcca ccgtcatcgt ccagacggcc ggcacccgtg acgccgagca gtggcttttc
     2521 acggtcgagc aggaggaatc tctgcatctg cccggcggga ccgtgagcgc gctcaagctc
     2581 acgcgcaacc cgcgcaagga gttcgaccag aagctggaaa tctggctggc ccccggcatg
     2641 gattacgtcc ccgtgcgcct gcgtctgaca cagccgaacg gagattcgct cgatcagctg
     2701 tggtcctcca ccgacaaggg ctgagccatc cttcgcctag tcttactacc gagggatccc
     2761 ctcggcgttt gcccggtgca ccttgaaact ggttcaaacg ttgctagata accaggcaga
     2821 aggatttagc accatgcaaa tgctctatga ctccgactcg ttcgtcgtcg tgcacatgca
     2881 ggccaatgaa ccccttgatg gggaaccccc gccgcgcatc gtgcgccacg gcttcgagat
     2941 cgtcgacaag cggtccaaca aggaggtcta cctggacggc tcctgggcgg aggctttcca
     3001 gcggcaaatc aatgcctggc agcagaacac cccgacgcag gaagaagtcg aggaaacact
     3061 ggacggctac gccgagttgg cgcaaaaccc gctcgtcatg cactgagagg cgctcatcag
     3121 cgccgaaagc cccatcgata cgaagtctcg acctcacagc gcaggcggcg gacccgacgg
     3181 caggcaccac cggcacgcca agttgtagaa ccttagccgt aatcgccatc tcggcgatcc
     3241 tgccgggaga cgtccccagc accaatgtgg cccagtactt ccggttccca acgccagcag
     3301 cgcgcggaat cgaaacgttt attgcagcgc tggggaggat gtagcatggg agccatgaat
     3361 cgttgcctgg cctcggcgct gctggtctgc ctggtgagcg ccgcctgcgc cgtccccgcc
     3421 gtttcggagg aaccggccgc ccgcctgcac cagctcctgc ccgccgacgc catcctgctg
     3481 ggtgaacagc acgacgcgcc ggatcaccag cgcattcacc gtgaggtcat cgaggccctg
     3541 gccgcgcgcg gcgccctggc ggcggtggct cttgaaatgg cgccccaggg cggctcgact
     3601 gccgggctgg cgcgcgacgc gacagaatcg gctgttcagg cggcgctgcg ttggaccgat
     3661 gcatcctggc cctgggcggt ctatgcaccg gccgtgatgg cggccgtgcg atcgggcgtg
     3721 ccggtactgg gcgcgaacct gccgcgcgcc gcgatgcgtc ttgccatggc cgatagccag
     3781 ctggattcgc tgctgccggg gccggcgctc aaggcccagc agcaggcgat ccggcagggc
     3841 cactgcgaca tgctgcccga aacgcagatt gcccccatga cgcgggtcca gatcgcgcgc
     3901 gaccgggcga tggcgcagac gctcgtcgcc tcggcctcgc cgggaaaatc ggtggtgctg
     3961 ctcgccggag gccgccatgt ggaccgccgg gtcggcgtgc ccctgcacct gccgcccggc
     4021 ctgcaaacca gggtcgtggc gctgcgcgcc ggcgccccgc ccgacgacag ccggcgagtg
     4081 gccgattcgg atgccatctg gaccacgccg cccgtgccgc cgaaggacta ctgcgcggac
     4141 atgaggcctc gcagctccgc ccagccggcc ggcgccgaga agccttgacc cgaaccggca
     4201 gacctgaaag tcaactggtg gtgaagctca ggcgaatgct ttcgccagcc attgattcga
     4261 agtgatcgag ccctcgtgcg cgtggtagat cgaggtgcgg cggttcgaga cgcgacagcg
     4321 gaacttgggc atggtgacgc gcgccaccac gttgtccgtg tagtaattct ggccatcgcg
     4381 cgcgatgtcg gacagcaatt cctgcgcgaa ctggcgattg aagcacatgg ttgaactcat
     4441 gccgcccggt gcgtgcacgg tgaaatcgac ttcgtgcgca tagcggaagt ccgacccctt
     4501 ggtaaagagc agcccgcagc gcgaggacac ggagaaatcg aactgctcca ggtctcgcag
     4561 gccgctggcg atgtgctcgc gcaggtagag gtcgtcgtgg tcggcccaga aaaaatgcgt
     4621 gcagccgctc tccagcagga acttcagcgg caccgcgtac cactggtgct gcgggatctt
     4681 cccgggggtg tgcagccacg caatgcgcga agcggtctgc aggtcggcca cggcccaggc
     4741 gtaggagtcc gggtgcccgt tctggtgcac gcagatgatg tcgggtgggc gggactgggc
     4801 cgcgagctgc agaacgcacg accgcagcag gtccggccgg ttgtaggtgg gcacgatcac
     4861 gccgacccgc gccacatcgg gccgtgaaac cgccggctga tcggaatcgg ccggcgcttg
     4921 cccggatccg gcgggcccct cctgcggatc aggcatgctg gcggatcgaa tcggcaagcg
     4981 tgttgaccag cgtgtcgatg tgctcttttt ccacgatgta cggcggtgcg atgaccagca
     5041 cgtcgccggc cggccgcacc agcgcgccct tgtggaagca gtcgatgtag acgtcgaagg
     5101 cgcgcttgcc cggcgagccg gcgatcggcg cgagctccac cgcggcggcc agccccaggc
     5161 tgcggatgcc gatgacgttc ggcaggccct tgaacgtcgc gtggaaggcg tcgcccagca
     5221 ccttgcccat ttcgccggcg cgctggaaca ggttctcctg cttgtacagg tccagcgtcg
     5281 cgatcgccgc cgcgcacgcc accgggtgcc cggaataggt gtagccatgg aagaactcga
     5341 ccacgtgctc gggcgcctcg gtcttcatca tcgcgtcgta cagcttgtcc cggcagatca
     5401 cgccgcccat cgggatcacg ccgttggtga cgcacttggc gaagttcagc atgtcgggca
     5461 ccacgccgta gtaatcggat gcgaagccgg tccccatgcg cccgaagccg gtgatcacct
     5521 cgtcgaagat cagcaggatg ccgtgcttgt cgcagatttc gcgcaggcgc ttcaggtagc
     5581 cctggggcgg caggtaccag ccggcgctgc ccgccaccgg ctcgaccatg atcgcggcca
     5641 cgttgctcgg gtcatgcagc ggcaggatgc ggttttcgag ctccagcagg atgtcttcct
     5701 gccagaccgg ctcgcggttg tggatgtagg cgttgttcac cgggtcgtgg atgaagcgca
     5761 tgtggtccac gcgcggcaga aaggcggcgc cgtacacctt ccggttggcc gggatgccgc
     5821 ccacgctcat gccgccgaag cccaccccgt ggtatccctt ctcgcggccg atgaagacat
     5881 tgcggtggcc ttcgccgcgg gcgcggtggt aggccagcgc caccttcagc gaggtgtccg
     5941 ccgcttccga cccggagttg cagaacagca ccttgttcaa gtcgcccggc gccatcgccg
     6001 cgatcttttc ggcggcctgg aacgccctgt cgttcgacgc ctggaacgcc gtggcgtagt
     6061 ccagcgtatc cagctgcttc ttgatcgcgt cgttgatggg cttgcggttg tggcccgctc
     6121 ccacgcacca gaggctggaa atgccgtcga tcacctgttt gccgtcatgg gtggtgaagt
     6181 gcatgccgtc ggccgcgaca aagatgcgag ggtccttgcg gaactggcgg ttgggggtga
     6241 agggcaacca gtggttgtcc atggaaaagt cgttgtaggc catggcaagc gtcctgtgcg
     6301 tactgaagaa agccattttg gcaccgcccg ggctcctggg cagtgcccgg ggcgctcctg
     6361 cgacaattca ggccgtatga acagaactta tatcctcacc ctgtcctgcc cggaccgccc
     6421 gggcatcgtg catgccgttt cgggtttcct gctggagcgg ggcggcaaca tcgaggaggc
     6481 cgcccagtac aacgaccatg acacgggcct gttcttcatg cgagtgcagt tcgcgtgcga
     6541 ccagctgacc ttcgaggacc tcaaggtcca gctgaaattc ttcgccgatc cgttcaagat
     6601 gacgtggaag ctgcatgcgc gcagcgagcc gatgcgcacg gtgatcctgg tcagcaagga
     6661 aggccactgc ctgaacgacc tgctgtttcg ctggaagagt gggctgctgc cgctggagat
     6721 cgccgcgatc gtttcgaatc accgggattt ctatcagcta gcggccagct acaacgtgcc
     6781 cttccaccat atgccggtcg cggcggccac gaaggcccag gtggaggccc ggcagttcga
     6841 gatcatcgaa gccgagggcg ccgaactggt ggtcctggcc cgctacatgc aaatcctttc
     6901 ggatgagttg tgccgcaagc tcgcgggccg cgccatcaac atccaccaca gcttcctgcc
     6961 cagcttcaag ggcgccaagc cgtattacca ggcgcacgac cgcggcgtga agctgatcgg
     7021 cgccaccgca cactatgtca ccgccgatct cgacgaaggc cccatcatcg aacaggacgt
     7081 ggcaagggtg gaccacagtg ccaccgtgga agacctcacg gcccagggcc gcgacaccga
     7141 aagccaggtg ctggcccgcg ccgtgaaatg gcacagcgaa catcgcgtgt tgatcaacgg
     7201 gcacaagacg gtgatcttca aatgacgagg cgaagtgcag cgcagcgttg tcatcccggg
     7261 ctgggttcgc ggaacgcccg gggcgtgcag ctgtccagtg cagcgagccc aatgccgggc
     7321 ctacgctatg cacgtaccga ggcatcacgc tccggcccgg cccgggaatc catggattgc
     7381 gggtcaggcc cgcaatgaca gccagatgac cgcccgcatt ccgccgatcc agtgcctcct
     7441 gactttcgag gcactggcgc gcttgcgctc ggtgacgctg gcggcggacg aactctgcgt
     7501 cacgcccagc gccgtgagcc accgcgtcaa gcagctcgag cagatcatcg gcaccaagct
     7561 gttcggccgc gccgacttct cgctcaccac cgaaggcaac gaatacctgg cgcatgtacg
     7621 cgaggggctc agcatcctgg agcgctttcc cggcaaggac agcgcccagg gcaaacgcaa
     7681 gctgcgcgtc gcggtgacgc ccaccttctc gcgctcgatc ctgatgccgc ggctgcgcgg
     7741 cttcatcgag gcctatccgg agatcgacct gacgctgcag gtgtcgatcc cgctgctgga
     7801 cgtggtcgcc gaggacgccg acctgatgat ccgctacggg accggccgct acgcggacgt
     7861 ggaacacctt tgcctcatga ccgacgaggt gacgccgctg gcgtctccga tgtacatccg
     7921 cgagaacggt ccgttcgaca cgatcgagga actgctggcc gccaaactca tcaagagccc
     7981 gctggagccc tggcgcacct ggttcctggc gcatggggtc gatgccggcg agcccggcga
     8041 cggttcctcg ttcaacgaca tcggcctcgt gtgcgacgct gcggcgcagg gcctgggcat
     8101 tgcgctggtg cgcttgaagc tggggcagcc gtggctggac aacggttcgc tggcgcgcat
     8161 ttccgagcgc aacgtgccca gcccgcacgc gcattacctg tgctggcgca ccggcaccat
     8221 ggatcgctgg gaatgcgccg cgtttgccga ctggctgaag aaaagcgtct gaaaaaaaca
     8281 aacaggtgcg gcggaaggcc cgcgcacctg tttgccctga tccgccgccc ttggaggagc
     8341 ggcattccgt cccgctttta gtgtttggtc gcagtgatga tctggaaatt gccgaagaag
     8401 aaggtcgtgc gttccacggt ccagccgccc atctcctgca gcagcaggag cgggtcatgg
     8461 gtgtcccaaa gcgcgaggcc gagcggctcg aacaccttga aataggtcca gctcagggcc
     8521 cgcagcaccc acagttcggg cttgtggaac tcgcccaggt acagcttgcc gccgggcttg
     8581 agcatgcggc cggcttcgct gagtgccttc gccttcagct cgtgcggcag ctcgtggagc
     8641 aggaagaaca tgacgttcgc gtcggcgatg ccttcttcct gcctcatcga ggtggcgttg
     8701 tcctcgacgt actggatctt ggcgccatag tggctcagct tgcttttggc atgaaccagc
     8761 tcgttcttga tgatgtcggc gatcaggatg cgttcggcgc cggactgcaa agaggcgtcc
     8821 acaaccctgg ggatcacgtt gccgaaagcg caggaggtga ccagcacctt cttgttcctg
     8881 aggtcggtcg ccctcaggcc ggcgacgatc ttggacacca ggcgggggta ttggaacaac
     8941 aggattgccg agatgatggg ctggtagtcc accatcttga tcaggcgcat gttcgagtag
     9001 atcggataca catgcgagct ctcgtgcccc gccccggcga gaccgcgaac gatcagcgca
     9061 ccgcgggtga ccacgaagaa gaacaggccc gttgccgcca aaacgaaaac caggccgaaa
     9121 atcgcattgc tcatcaaggt actcattgcc ccgactcctg ttgtctcgcc cgaaccgccc
     9181 gggccggcac ttcacgttca atcctgctca ggatggtcat ttccgcccac ttacatctat
     9241 tcgcatccgg tcatggcatc gtgccagcgg ctggcggcgc cccaattgat ttagcgcagt
     9301 acggatcatc ggaactttcc tatcttttcc ctaggactgg cggcatccgc cgcgcgagcg
     9361 gcggtggcct tttttcagct gcatcatgca aaaagctcat accgggcctc ggcgacttgc
     9421 cctaaagtgc gtccatgaat gccgcccccg aacccagctt gcttggccgt gccgagcgcc
     9481 agtcgcaggt ggtgggggcg ctgcaggccg tccttccgcc ggacgccttg ctctggcatg
     9541 cggaggacac caccccttac gagtgcgacg ggctgaccgc ctaccgccag cgaccgttag
     9601 tggtcgctct gccggagagc gagacccagg tccaggcggt gctgcgtacc tgccatgcgc
     9661 tggacgtgcc ggtggtagcc cggggcgcgg gcaccgggct gtccggcggc gccatgccgc
     9721 atgccctggg cgtcaccttg tcgctggccc gcttcaaccg gatcctcaag ctcgatccgg
     9781 cggcccgcac ggccctggtg caatgcggcg tgcgcaacct ggccatcagc gaagccgccg
     9841 cggcctatgg cctttactac gcgcccgacc cgtccagcca gatcgcctgc accatcggcg
     9901 gcaacgtcgc cgagaattcg ggcggcgttc attgcctgaa gtacggattg acgctgcaca
     9961 acgtgatcaa ggtgcgcggt ttcaccgtgc agggcgagcc ggtggatttc ggcagcgatg
    10021 cgctggactg cgcgggcttc gacctgctca gtgccatcgt gggcagcgaa ggcatgctgg
    10081 ccgtggtcac cgaggtcacc gtcaagctgg tgcccagtcc gctgctggcg cgctgcatca
    10141 tggccagctt cgacgacgtg cgcaaggccg gcgacgcggt ggccgccgtg atcgccgccg
    10201 gcatcatccc ggccggcctg gagatgatgg acaagcccat gacggcggct gtggaggatt
    10261 tcgtccatgc cggctacgac ctgacggcgc aggccatcct gctgtgcgag agcgacggca
    10321 cgcccgagga agtcgaagag gagatcgctc gcatgagcga ggtcttgcgc tcccacggcg
    10381 ccaatgccat cgcggtgagc cgcgacgagg ccgagcggct gaaattctgg agcggccgca
    10441 agaacgcctt cccggcctcc gggcgcctca gccccgacta catgtgcatg gattcgacca
    10501 ttccgcgcaa gcgcctggcc gacatcctgc tggcgatcca gcaaatggaa aagaaatacg
    10561 cgctgcgctg tgccaacgtg ttccatgccg gcgacggcaa cctgcacccg ctgatcctgt
    10621 tcgatgccaa cgacgccgac cagttgcacc gcgccgagct gttcggcgcc gagatcctgg
    10681 aaaccagcgt ggccatgggc ggcaccgtga ccggagagca tggcgtcggc gtggaaaagc
    10741 tcaactcgat gtgcgtgcag ttcagcacgg ccgaatgcga gcagatgttc ggggtcaagc
    10801 gcgccttcga cgccaagggc ctgctcaacc ccggcaaggt ggtaccgacg ctcaatcgct
    10861 gcgccgaata cggaaagatg ctggtgcgcg gcggccggct cgcacatccg gacctgccgc
    10921 ggttctaggc ggctaacgca ggcactggtt gtcgtccacc aggttgtcgg tgccctggcg
    10981 acgctgccgc gcctgctttt gctcttcgcc gatcagcagg gtgacgaaat tcgcgagcgt
    11041 attcgtctcc aggtcggcca gcgcggccat cacatcgccg cggtcctcgc tggggaacaa
    11101 gtcgatgatc cgatcgaccg ttgaggcgcc ggtaggggcc ggccttggcg cgggcgtggg
    11161 gtctggtgtc cgggccggtg tgggcgctgg cgtcggctcg ggtgttggag cgggcgtagg
    11221 cgccggggtc ggctcgggcg tcggcgcggg cgtaggcgcc ggggtcggcg cgggcgtggg
    11281 cgctggagtc ggctcaggcg tcggcgcggg cgtgggagca ggcgtcggcg caggcgtcgg
    11341 cgcaggcgtc ggttcaggcg tcggttcagg cgtcggcgcg ggcgtcggct caggcgtcgg
    11401 ctgtggcgtg ggtgaagctg tcggcgccgg aaccggcaca gtggccgtcg cccggatggc
    11461 ctggggcggg ccggcgacca ccgacagtgc gaatccggcc accgccggga gtgccccacc
    11521 cgcgaccgat cccgtggcgc tgacgatgtc gaatgtatcg cccacggcga tgctgggcga
    11581 aatcaggttc gcgttgacgg tcacgtcgct gctggcgagg ttgacgtcgc cgggagtgcc
    11641 cgtgatgctg agaacgtcgt aggtcgatcc cgggaccgaa cggttgccga tgtcgaggta
    11701 caggctggtg ccggaatcga ggttgaccgt cgcggcgtcc agggtcaggg tgcccatgct
    11761 gcccaagcct ccgggcctca gttgacccgc attgttgaac gtgcctgtgc ccagtttcaa
    11821 ggtgccggag ccggagatga cacccgtcga cgtgttggcc agcgttgtgc cgttgttgtc
    11881 cagcgtggcg ccggaggcca gcaggatgag gccggagttg ctcgagaacg ccgcactgcc
    11941 gaactgcagc gtgccattcg aaacagccag cgttccggta ttgctgaagc tgaccccatc
    12001 gagcagactg aggccggtgc ccgcactctt ctttagcacg ccggaattga cgatcggcac
    12061 cgggctgggg ctgggggccg gggtgaagcc gccgttgttc tggatgtcga acgtgccggc
    12121 gttggtcagc gaggcgccgc tgtccagtgc gatctgcccg gtcccggaca ttttgatgat
    12181 gccgctgctg gcagtcgcca gcggccgcgc cagcaatacc ggtcccgtga tgctcgcgct
    12241 ggccgacgga cccaaggtcg tgctgccgga accggtcatg ctgcctccgg accatgtcag
    12301 gctgccgccc acggtcaggt tgcccgcgcc ggtgatcgtc ccggcgcctg cgagattgag
    12361 ggaagaaacc gagacatcgg ctccgatggc caaagtcccc tggatgtcca ggggggtacc
    12421 ggacaagctg gtattcagag tggtcgaccc ggcgacctgg gcggcggctg cactcagcgt
    12481 gctggtaccg gtgccgctga gggtggcacc gttgaacaga atgtcccccg cagcggtcaa
    12541 gctgttgctg cccgtccccg cgtcc
//
LOCUS       DASWIY010000373         6146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2855064, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000373 DASWIY010000000
VERSION     DASWIY010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2855064"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3050)
                     /locus_tag="VGA59_13420"
     CDS             complement(<1..3050)
                     /locus_tag="VGA59_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX9720719.1"
                     /translation="MSMSTPLSLPAYAELHCLTNFSFQRGASHPGELVERAWELGYAA
                     LAITDECSVAGVVRAYAALKDMKLPADDPFKLILGSELRFDGFRLVALARNVTGWGNL
                     CEFITAARLHAKKGEYIVSRDHSDFSLLQECEILVAPARDGGDAVDAGDAVALQAIAL
                     WAKRHFGSRCWLAAELSLGLDDALWLDTLQRVGAATGVPLVAAGDVHMHLRSRKALQD
                     VITAVREGKVVSECGLALQASAERYLRPRVRLGGIYPGELLANTLLVAARCDFSLESI
                     KYQYPLETVTAGMTPAQALRQFTIEGARKRYPKGVPWKVRRQLVHELRLIAACKYEMY
                     FLTVHDIVRHARERKILCQGRGSAANSAVCYCLHVTEVDPARANLLFERFISKERKEP
                     PDIDVDFEHERREEVIQYIYTKYGRDRAAIAAVVASYRSRSAIRDVGKALGIPPGLVD
                     AFAKEHYWFDEEGLQVRRLCERAAETGADIGELRMQQWLELTETLRGFPRHLSQHVGG
                     FVLTQGKLTRLVPVENAAMPDRSIIQWDKDDLEDMGLLKVDVLALGMLTALRRCLDFV
                     SRRRGYPFALQDIEDRDVETYDMICRADTVGVFQIESRAQMSMLPRLRPRVFYDLVIE
                     VAIVRPGPIQGGMIHPYLKSRERLRKGEKIRYENDALKPALERTLGIPIFQEQVMQIA
                     MIAAGFSPDEADRLRRSMAAWKRRGGVHQFQDRLIDGMLARGYTREFAESIFKQILGF
                     GEYGFPESHAASFALLAYDSSWLKRHESECFLAAMLNSQPMGFYSPSQLVQDGRRHGV
                     QVLPPDVSYSDWDCTLEKLGSDSNSFRSAELGSDSNSSRPAVRLGLRMIGSLSEAGAQ
                     RVVAARASAPFTSTEDMALRAHLDVKDLNALASGDALLSLSGHRRQQVWDAAAQRGVP
                     ALLQNAPVNEEALELPAAPEGEEIVFDYASLGLTLRRHPLALLRPRLARMKLLSAAQL
                     HDLPSGRRVRACGIVTVRQQPQTAHGTIFVTLEDETGPVNVIVW"
     gene            complement(3047..4423)
                     /locus_tag="VGA59_13425"
     CDS             complement(3047..4423)
                     /locus_tag="VGA59_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX9720720.1"
                     /translation="MYWIALLPSHEDERCAWGWRALRFTPRVAQVDEALLLEASASLR
                     LWGGRKRLLRRLLDECEPLAGVQWAQGATSLIALGLLRLKLRGETAPARLPQDLPLEV
                     LSAAREHALILERTGCRSWGDLHALPRAGVARRFGAGLLDALDAAYGERPERYPWLQL
                     PEAFDMKVELASLATTAPELMWSAQRLLGQLQVWLQARHRGVLAFELEWTLDLRRLNG
                     VRLPSHEQLVVRTAQPTQDIAHLRRLAGEHLSRSSLSAPANYLRLRSLETLPWPGANK
                     SLLPEENVKGERLHQLVERLSVRLGQGNVVVPVAQADHRPERMQRWEAARSSSAIAGV
                     SAGNLAKGSVCAPGSSTPASRPGNKRRLPCDALYPPWLLPQPLRLEVQGEKPQYHGPL
                     RLLAGPQRLETGWWDAAAQGPALRDYFIARSDEAGLLWVYRERLAARSGGEAEIAPVR
                     WFLHGIYA"
     gene            complement(4392..5147)
                     /gene="imuA"
                     /locus_tag="VGA59_13430"
     CDS             complement(4392..5147)
                     /gene="imuA"
                     /locus_tag="VGA59_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HEX9720721.1"
                     /translation="MPARLSLIEPASLPHVWRVEDLACQEAVLPTGHAALDAQLPGGG
                     WPVGSMVEVLQERAGPHLWQLLLPGLAQAVRKQAGPVVLVNAPFEPFGPSLVAQGLPG
                     ERLLCIKADKPAARLWAAEQALRCADVAALLAWLPQARSAELRRLHMAAQQQDRLLFV
                     FRGLNARHDASPARLRLLLEGVDQLELHILKRRGPPLETALALPGHSARLVALLEARK
                     RRGSGTLSTALLPAPVVPLPRRSHVLDRTAALT"
     gene            5265..5567
                     /locus_tag="VGA59_13435"
     CDS             5265..5567
                     /locus_tag="VGA59_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEX9720722.1"
                     /translation="MRVIALKNIRDFCVRQPQAAQALKAWTQEARSASWKSPQDIKKR
                     YSSASFLGRNRVVFNIKGNEYRLVVAVAYQFEAVYIKFIGTHAQYDAVDAETIEME"
     gene            5575..5937
                     /locus_tag="VGA59_13440"
     CDS             5575..5937
                     /locus_tag="VGA59_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9720723.1"
                     /translation="MDVRPIHNKADYKAALKATSSYFDNQPEPGTDDADRFEILLTLI
                     QAWESKHFPVELPDPIEAIKFRMEQSGLAIRDLEPMIGRSNRVYEVLNRKRPLTLAMI
                     RRLHRGLGIPAEVLVAGI"
     gene            5972..>6146
                     /locus_tag="VGA59_13445"
     CDS             5972..>6146
                     /locus_tag="VGA59_13445"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX9720724.1"
                     /translation="MNKPNSSLRSADFARDSHELGRALIGATLLVDGVGGRIVETEAY
                     DRHDPASHSFSGPT"
BASE COUNT         1092 a         2089 c         1959 g         1006 t
ORIGIN      
        1 ttccagacga tcacgttgac cggcccggtc tcgtcctcca gcgtgacgaa gatggttccg
       61 tgcgccgttt gcggctgctg gcgcacggtg acgatgccgc aggcgcgcac gcgccggccg
      121 ctgggaaggt cgtgcaattg cgcggcgctg agcaatttca tgcgcgccaa cctcgggcgc
      181 agcaaggcca gaggatgccg gcgcagcgtc aggcccagcg aggcgtagtc gaatacgatt
      241 tcctcgccct cgggagcagc gggcaattcc agcgcttcct cgttgacggg ggcattctgc
      301 agcaatgcgg gcacgccgcg ttgcgccgcc gcgtcccaga cttgctgtcg ccggtgaccc
      361 gaaagagaga gcagcgcatc gcccgaggcc agggcgttca gatccttcac atcgagatgc
      421 gcgcgcaagg ccatgtcctc ggtgctggtg aacggcgcgc tggccctcgc cgcgacgacg
      481 cgttgggccc ctgcttcgct gaggctgccg atcatccgca ggcccagccg cacggccggc
      541 cgcgaagaat tggaatctga ccccaattct gcggatcgaa aagaattgga atctgacccc
      601 aatttctcca gggtgcagtc ccagtcgctg tacgacacgt cgggcggcag cacctgcacg
      661 ccgtggcggc ggccgtcctg caccagctgc gaaggcgaat agaaacccat gggctgcgag
      721 ttgagcatcg cggccaggaa acactcgctc tcgtggcgtt tcaaccagct gctgtcgtag
      781 gccagcagcg cgaagctggc ggcatggctt tccggaaagc catactcgcc gaaacccagg
      841 atctgcttga agatgctctc ggcaaactcg cgcgtatagc ccctggccag catgccgtcg
      901 atcaaccggt cctggaactg gtgcaccccg ccccggcgct tccaggccgc catcgagcgg
      961 cgcaggcgat cggcctcgtc gggcgagaag ccggcggcga tcatggcgat ctgcatcacc
     1021 tgctcctgga agatcggaat gcccagcgtg cgctccagcg ccggcttgag ggcatcgttc
     1081 tcgtagcgga tcttctcccc ttttcgcagg cgctcccggc tcttgaggta gggatggatc
     1141 atgccgccct ggatcggccc agggcgcacg atggcaacct cgatcaccag gtcgtagaac
     1201 acgcgtggcc gcaagcgcgg cagcatcgac atctgggccc ggctctcgat ctggaacacg
     1261 cccaccgtgt ctgcgcggca gatcatgtcg taggtctcca cgtccctgtc ctcgatgtcc
     1321 tgcagggcga acggatagcc gcggcgccgg ctcacgaaat ccaggcagcg gcgcagcgcg
     1381 gtgagcatgc ccagggccag gacgtcgacc ttgagcaggc ccatgtcctc caggtcgtcc
     1441 ttgtcccact ggatgatgga gcggtcgggc atggccgcgt tttccaccgg aaccagccgc
     1501 gtgagcttgc cttgggtgag cacgaaaccg cccacgtgct ggctcaggtg gcgcggaaag
     1561 ccgcgcagcg tttcggtgag ctccagccat tgctgcatgc gcagctcgcc gatgtccgcg
     1621 ccggtctccg ccgcccgctc gcaaagccgc cgcacctgca ggccctcttc gtcgaaccag
     1681 taatgctcct tggcgaaagc atcgacaagg ccaggcggaa tgcccagggc cttgcccacg
     1741 tcgcggatgg cgctgcgcga gcggtagctg gcgaccaccg ccgcgatggc ggcgcggtcc
     1801 cggccgtatt tggtgtagat gtactggatg acttcttcgc gccgctcgtg ttcgaagtcc
     1861 acgtcgatgt cgggcggctc cttgcgctcc ttgctgatga agcgctcgaa cagcaggttg
     1921 gcacgcgccg ggtccacctc ggtcacgtgc aggcagtagc agactgccga attggcggcc
     1981 gagccgcggc cctggcacag gatcttccgc tcgcgggcat ggcgcacgat gtcgtgcacc
     2041 gtgagaaagt acatctcgta cttgcaggcc gcgatcagcc gcagctcgtg cacgagctgc
     2101 cggcgcacct tccagggcac ccccttcgga taacgtttcc tggcgccttc gatcgtgaac
     2161 tgccgcagcg cctgggcggg cgtcatgccc gctgtcaccg tctccagcgg gtactggtat
     2221 ttgatggact ccaggctgaa gtcgcagcgc gcagccacga gcaaagtgtt cgccagcagt
     2281 tcgcccgggt agatcccgcc cagccggaca cgcgggcgca gatagcgctc ggcactggct
     2341 tgcaaggcca ggccgcactc gctcacgacc ttgccttcgc gcaccgcggt gatcacgtcc
     2401 tgcagggcct tgcgcgagcg caggtgcatg tgcacgtcgc ccgctgccac caggggcaca
     2461 cccgtcgcgg cgccgacgcg ctgcagggtg tccagccaca gcgcatcgtc caggcccagc
     2521 gacaactcgg ctgcgagcca gcagcgtgat ccgaaatggc gcttggccca aagtgcgatg
     2581 gcttgcagcg ccaccgcatc cccggcgtcc accgcatccc cgccatcgcg cgcaggcgcc
     2641 accagaatct cgcactcctg cagcaagctg aaatcgctgt gatcgcggct cacgatgtac
     2701 tcgccttttt tcgcgtggag ccgggccgcg gtgatgaatt cgcacaggtt gccccagccc
     2761 gtcacattgc gcgccaacgc caccagccgg aaaccatcga agcgcaattc gctgcccagg
     2821 atcagcttga acgggtcatc cgcaggcagc ttcatgtctt tcagcgccgc gtaggcgcgc
     2881 accacgccgg ccaccgaaca ttcatcggtg atcgccagcg ccgcgtagcc cagttcccag
     2941 gcgcgctcca cgagttcccc cgggtgggag gcaccgcgct ggaagctgaa gttcgtcagg
     3001 cagtgcagct cggcatacgc gggcagcgaa agtggcgtcg acatgctcat gcgtagatcc
     3061 catgcaggaa ccagcgcacc ggcgcgatct cggcctcgcc gccggagcgc gccgcaagcc
     3121 gctcacggta cacccacagc agcccggcct cgtcgctgcg cgcgatgaag taatcgcgca
     3181 gggccgggcc ttgcgctgcc gcgtcccacc agccggtctc cagccgctgg ggcccggcca
     3241 gcaggcgcag cggcccgtgg tactggggct tttcgccctg cacttccagc cgcaggggct
     3301 gcggtaacag ccaaggggga taaagcgcat cgcatggcaa ccggcgtttg ttgccgggcc
     3361 tggacgccgg cgtgctgctg cccggcgcac agacgctgcc cttggcaagg ttccccgcgc
     3421 taacgcccgc aatggcgctg ctgcttctcg ccgcctccca gcgctgcatg cgctcgggcc
     3481 ggtgatcggc ctgcgctacc ggcaccacca cattgccctg cccgagccgc acactgagcc
     3541 gctccaccaa ttgatgcagg cgttcgccct tgacgttctc ttcgggcagc aggctcttgt
     3601 tggcaccggg ccagggcagg gtttcgagcg agcgcagccg caggtaattg gccggggccg
     3661 acaagctgga acgggacaga tgctcgcctg ccaggcgccg caggtgggcg atgtcctgcg
     3721 tgggctgggc cgtgcgcacc accagttgct catgactggg cagccgcacg ccattgaggc
     3781 gccgcaagtc cagtgtccat tcgagttcga atgccagcac gccgcgatgg cgcgcctgca
     3841 gccagacctg caattggcca agcaggcgct gcgcgctcca catcagttcg ggagcggtgg
     3901 tggccagcga agcgagctcg accttcatgt cgaacgcttc gggaagctgg agccagggat
     3961 aacgctcagg ccgctcgcca taggcggcat ccaatgcatc gagcaggccc gcgccgaagc
     4021 gccgcgccac acccgcgcgc ggcaaagcgt gcagatcgcc ccagctgcgg caaccggttc
     4081 gttccaggat cagggcgtgc tcgcgagcgg cactcagcac ttccaggggc agatcctgtg
     4141 gcagccgggc cggcgccgtt tcgccgcgca acttcaggcg cagcaagccc agagcgatga
     4201 gcgaggtagc accttgcgcc cattgcacgc cggccagggg ctcgcattca tcgagcagcc
     4261 ggcgcaacaa tcgcttgcgg ccgccccaca accgcagcga cgcggacgct tcgagcaata
     4321 gcgcttcatc gacctgcgca acccggggcg tgaaccgcaa ggcgcgccag ccccaggcgc
     4381 agcgttcgtc ttcatgtgag ggcagcagtg cgatccagta catgcgatct cctcggcagc
     4441 gggacaaccg gcgccgggag cagcgccgtg gaaagcgttc ccgacccgcg ccgtttgcgc
     4501 gcttcgagca gcgccaccag ccgcgcggaa tgccctggca aagccagcgc cgtttcaagc
     4561 ggcgggccgc gccgcttgag gatgtgcagc tcgagctggt ccactccttc gagcagcagc
     4621 cgcaaacggg ccggcgaagc atcgtgacgc gcattcaagc cgcgaaaaac gaacagcagc
     4681 cggtcttgct gctgcgccgc catgtgcagg cggcgcagct cggcgctcct ggcctggggc
     4741 aaccaggcca aaagcgccgc cacgtcggcg cagcgcaagg cttgttcggc ggcccagagc
     4801 cgggccgcgg gtttgtcggc cttgatgcaa agcagtcttt cgcccggcag cccttgcgca
     4861 accaggctgg ggccgaacgg ctcgaacggc gcattcacca gcacgacagg acctgcctgc
     4921 ttgcgaacgg cctgcgccag gccgggcagc agcaattgcc acagatgcgg cccggctcgt
     4981 tcctgcaaca cctccaccat gctgccgacg ggccagccgc cgcccggcag ttgtgcgtcc
     5041 agcgctgcat ggccggtggg cagaactgcc tcttggcacg cgagatcctc cacccgccag
     5101 acatgcggca gggaggcggg ttcaatcaga cttaaacggg cgggcacagg ggaaatttcg
     5161 ggtaggatac tgtttgttta tacagtcttt gcgaagcttt aggaagtcgt ccttcttctt
     5221 aaaaagccct tgaaagcgac agtcccatat tgggactaaa atccatgcgt gtgatcgctc
     5281 tcaaaaatat tcgcgatttt tgtgttcggc agccccaggc agcccaggca ttgaaagcgt
     5341 ggacacagga agcgcgcagt gcctcgtgga aatcgcccca agacataaag aaacgctaca
     5401 gcagtgcgag cttcctggga cgcaaccgcg tggtgttcaa catcaagggc aacgagtatc
     5461 ggctggtggt ggcagtggcc tatcagttcg aggccgtcta catcaagttc atcggcaccc
     5521 atgcgcaata cgatgccgtg gatgcggaaa ccatcgaaat ggagtaacgc agccatggac
     5581 gttcgcccca tccataacaa agccgattac aaggcagcgc tcaaggcgac ctcttcctat
     5641 ttcgacaacc agcccgagcc gggcacggac gatgcggacc ggtttgaaat cctgctcaca
     5701 ctgattcagg cttgggaaag caagcacttc cccgtagagc tgcccgaccc catcgaagcg
     5761 atcaaatttc gaatggagca gtcgggactg gccatcaggg acctggagcc gatgatcggc
     5821 aggtctaacc gggtgtatga ggtactcaat cgcaaacgcc cgctgacgct ggccatgatc
     5881 cgtcgcctgc accgcggcct gggcattccg gccgaagtgc tggtcgccgg gatttgagcc
     5941 tcgccgcgct ggagcccatt tgctcccttt gctgaacaag ccgaactcgt cgctcaggtc
     6001 tgctgacttc gcgcgtgact cgcacgagct gggccgcgcg ctgatcggcg ccacgctgct
     6061 cgtcgacggc gtcggagggc gcatcgtgga aaccgaggca tacgatcgcc atgaccccgc
     6121 gtcgcacagc ttttccggtc ccacgc
//
LOCUS       DASWIY010000374         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2857076, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000374 DASWIY010000000
VERSION     DASWIY010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2857076"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..>2021
                     /locus_tag="VGA59_13450"
     CDS             12..>2021
                     /locus_tag="VGA59_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9720725.1"
                     /translation="MKTIKYELQDGIATITFDEEGSPVNTMCQQWQQDLTDVTAQVVK
                     DKEAIKGIILASAKSTFFAGADLKGTMRLQPSDAQQAFREIEQTKKDFRTLETLGKPV
                     VSCLNGSALGGGWEVALVGHYRIAVDDPKIQFGLPEITLGLIPGASGITKMTRLLGLM
                     GAQPYILESKLFPPREALELGLVHELVPQASALRPAALAWTAKHASSAAPAHHPWDDK
                     NYKMPGGTPNHPKIAGMLAVAPAMLKQKTRGLYPAPEYALAAMVEGALVDFDTALRIE
                     SRYLAKLLVSPVAKNMINTFFFNMNAIKSGQSRPKDVPRSKVQKVGILGAGMMGGGIA
                     YVQAGRGIATVLRDLTMDKAETGKAYSGKITQTRVEKGKMSPHDQAALLSRITATDSI
                     EDLRGCDLIIEAVFENRELKARVTREAEPMLAPGGFFASNTSTLPISGLAKASSRPEK
                     FVGIHFFSPVDKMKLVEIIRGKQTDDETVARAFDYVQSIGKIPIVVNDSRGFFTSRVF
                     GTFVMEGAAMVGEGIPAAVVEQAGIQAGMPVGPLAVLGETALSLSVHVMDQTRADYRA
                     EGGTYMAPPGELLIERMVKEFDRNGRAAGAGFYEYPQEKGAKKYLWPELKKLFERPGV
                     TWDMQDLTDRLLYRQAVETARCLCENVLTTVHDANIGSIFGIGFPA"
BASE COUNT          402 a          671 c          656 g          292 t
ORIGIN      
        1 caagggaaac catgaaaacc ataaagtacg aactgcagga cggcattgcc accatcacct
       61 tcgacgagga gggctcgccg gtcaacacca tgtgccagca atggcagcag gacctgaccg
      121 acgtgacggc gcaggtggtg aaggacaagg aggccatcaa gggcatcatc ctggcttctg
      181 cgaaaagcac tttctttgcc ggcgccgatc tcaagggcac gatgcggctg cagccatcgg
      241 acgcgcagca agcgtttcgc gagatcgagc agaccaagaa ggacttccgc accctggaga
      301 cgctgggcaa gccggtggtg agctgcctca acggatcggc cctcggcggg ggctgggagg
      361 tggcgctggt cggccattac cgcatcgccg tggacgaccc caagatccag ttcggcctgc
      421 cggagatcac gctggggctg atccccggcg cgtccggcat caccaagatg acgcgcctgc
      481 tgggcctgat gggcgcgcag ccctacatcc tggagagcaa gctgttcccg ccgcgcgagg
      541 cactggaact gggcctggtc catgagctgg tgccccaagc ctccgcgctc aggcccgcgg
      601 ccctggcctg gaccgccaag cacgcgagca gcgcggcgcc ggcccaccat ccctgggacg
      661 acaagaacta caagatgccg ggcggcacgc ccaaccatcc caagatcgcg ggcatgctgg
      721 ccgtcgcccc ggccatgctc aagcagaaaa cgcgcggcct ctatcccgcg ccggaatacg
      781 cgctggccgc catggtggaa ggcgccctgg tcgatttcga tacggcgctg cgcatcgaga
      841 gccgctatct cgccaagctg ctcgtgagcc cggtcgcgaa gaacatgatc aacacgttct
      901 tcttcaatat gaacgcgatc aagtccgggc aatcgcgtcc caaggacgtg ccgcgcagca
      961 aggtgcagaa ggtgggaatt ctcggcgccg gcatgatggg cggcggcatc gcctacgtcc
     1021 aggccggccg cggcatcgcc acggtgctgc gcgatctcac gatggacaag gctgagaccg
     1081 gcaaggcgta cagcggcaag atcacgcaga cgagagtcga gaagggcaag atgagccccc
     1141 acgaccaggc cgccctgctc tcgcgcatca ccgcgaccga ttcgatcgag gacctgcgcg
     1201 gctgcgacct catcatcgag gcggtattcg agaaccgcga actcaaggcc cgcgtgacgc
     1261 gcgaggccga gccgatgctg gcgcccggcg gcttcttcgc gagcaacacc tccacgctgc
     1321 ccatcagcgg gctggccaag gccagcagcc ggccggagaa attcgtcggc atccacttct
     1381 tcagcccggt ggacaagatg aagctggtgg agattatccg cggcaagcag accgacgacg
     1441 aaacggtggc acgcgccttc gactacgtgc agtccatcgg caagatcccg atcgtggtca
     1501 acgattcgcg cggcttcttc acgtcgcggg tcttcggcac cttcgtgatg gaaggcgcgg
     1561 ccatggtcgg cgaaggcatc cccgccgccg tcgtggaaca ggcgggaatc caggccggca
     1621 tgccggtggg gccgctcgcg gtgctgggcg aaacggccct gtcgctgtcg gtgcacgtga
     1681 tggaccagac ccgcgccgac taccgcgccg agggcggcac ctatatggcg ccccccggcg
     1741 agctactgat cgagcgcatg gtcaaggaat tcgaccgcaa cggccgcgcc gcgggcgctg
     1801 gtttctacga gtacccgcag gagaaagggg ccaagaagta cctctggccc gaactgaaga
     1861 agcttttcga acgccccggc gtcacctggg acatgcagga cctgacggac cggctgctct
     1921 accgccaggc agtggaaacc gcacgctgcc tctgcgaaaa cgtgctgacc acggtgcacg
     1981 acgcgaacat cggctcgatc ttcggcatcg gctttcccgc c
//
LOCUS       DASWIY010000375         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1756643, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000375 DASWIY010000000
VERSION     DASWIY010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756643"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /gene="miaB"
                     /locus_tag="VGA59_13455"
     CDS             complement(<1..1000)
                     /gene="miaB"
                     /locus_tag="VGA59_13455"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX9720726.1"
                     /translation="MNEYDSDKMADVMGAAQGYQTTQNVEEADLILFNTCSVREKAQE
                     KVFSDLGRVKHLKREGVLIGVGGCVASQEGAAIIERAPYVDVVFGPQTLHRLPELLRR
                     REQLQRPQVDISFPEIEKFDNLPPARVEGPTAFVSIMEGCSKYCSYCVVPYTRGEEVS
                     RPFEDVLVEVAGLADQGVKEVTLLGQNVNAYRGRMGDTAEIADFALLIEYVADIPGIE
                     RIRYTTSHPNEFTQRLIDVYAKVPKLVNHLHLPVQHGSDRILMAMKRGYTAMEYKSTV
                     RKLRAIRPALALSSDFIVGFPGETEEDFGKLMKLVQDVGYDSSFSFIFSPRPGTPAA"
     gene            complement(1136..1564)
                     /locus_tag="VGA59_13460"
     CDS             complement(1136..1564)
                     /locus_tag="VGA59_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720727.1"
                     /translation="MNRCNKTLARLTLAACAALAGTSLFFPGAASAQGIQREAPKDVV
                     LGRLVVTAPPQITLDGKPDRLSPGSRIRDLNNMLVLSAGIVGKDLPVVYRRDAAGLVH
                     EAWVLTDEEYSKLGGASGAGGADGYKRFAELLAIIFGARR"
     gene            1723..1986
                     /locus_tag="VGA59_13465"
     CDS             1723..1986
                     /locus_tag="VGA59_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX9720728.1"
                     /translation="MLSILGTIVVGFIVGLIARAVKPGDDKLGLIMTTLLGIAGAFIA
                     RYAGVALGWYQQNEPAGWIASVIGAVVLLFIYSLVKGKSRDNV"
BASE COUNT          350 a          685 c          679 g          404 t
ORIGIN      
        1 tggcggccgg cgtgccgggc cggggactga agatgaagct gaacgagctg tcgtagccca
       61 cgtcctggac cagcttcatc agcttgccga agtcttcttc cgtctcgccc gggaagccca
      121 cgatgaagtc gctggacagg gcgagtgcgg ggcggatcgc gcgcagcttg cgcaccgtgc
      181 tcttgtactc catcgccgtg tagccgcgct tcatcgccat caggatgcgg tcgctgccgt
      241 gctgcaccgg caggtgcagg tggttcacca gcttgggcac cttggcgtac acatcgatca
      301 ggcgttgcgt gaactcgttg ggatggctgg tggtgtagcg gatgcgttcg atgccgggga
      361 tgtcggccac gtactcgatc agcagcgcga agtcggcgat ttccgctgtg tcgcccatcc
      421 tgccgcggta tgcgttgacg ttctggccca gcagtgtgac ctccttcacg ccctggtcgg
      481 ccaggcccgc cacctcgacc agcacgtcct cgaacggacg cgagacttcc tctccgcgcg
      541 tgtacggaac gacgcagtag ctgcagtatt tcgagcagcc ttccatgatg ctgacgaatg
      601 cggtgggtcc ctccactcgc gccggcggca ggttgtcgaa tttctcgatc tccggaaagc
      661 tgatgtcgac ctgtgggcgt tgcagctgct cgcgccgtcg cagcagttcg ggcagccggt
      721 gcagcgtctg cggcccgaac accacgtcca catagggcgc gcgctcgatg atggccgcgc
      781 cttcctggct ggcgacgcag ccgcccacgc cgatcaacac cccttcgcgc ttgaggtgct
      841 tgacccggcc gaggtcgcta aacaccttct cctgcgcctt ttcgcgcacc gagcaggtat
      901 tgaacaggat caggtcggct tcctcgacgt tctgcgtcgt ctggtaaccc tgcgcggcgc
      961 ccatcacatc ggccatcttg tccgagtcgt attcgttcat ctggcagccg aaggtcttga
     1021 tgaaaacctt cttggggacc tgcgcctgac cgttggcggc cgggccggct gcacggccgc
     1081 ccgacggtgg ctccgcttgc ttgaagcggt cctgcgctgt gctcatgtcg gcctcctaac
     1141 gacgcgcgcc gaagatgatg gccagcagtt ccgcgaagcg cttgtaaccg tcggcgccgc
     1201 ccgctcccga tgccccgccg agtttgctgt actcctcgtc ggtcagcacc caggcctcgt
     1261 gcacgaggcc cgccgcatcg cgccgataga ccaccggcag gtccttgccg acgatcccgg
     1321 cggaaagcac tagcatattg ttgaggtcgc ggatgcgcga ccccggggaa agccggtcgg
     1381 gcttgccatc cagcgtgatc tgcggcggcg ccgtgacgac caggcgtccc agcacgacgt
     1441 ccttgggcgc ctcgcgctgg atgccttggg ctgaagcggc gccaggaaag aaaagggacg
     1501 tgcccgccag cgccgcgcaa gcggccagtg tgaggcgggc cagggtcttg ttgcagcggt
     1561 tcatggtatc catccagagg ctgtcgaaga aaaacaaggc gccgcagcgg cgcctcccgg
     1621 gctgatcgcg aagtttacca aagcgtaaga acgtcactgc tgagccccgc gcgtgccagc
     1681 cggcatctaa agtgcactct ccaccactga aggagttatt ccatgctttc gatcctgggc
     1741 accatcgttg tcgggttcat cgtcggcttg attgccaggg ccgtgaagcc cggagacgac
     1801 aagctcggcc tgatcatgac cacgctgctc ggaatcgcag gggccttcat tgcccggtac
     1861 gccggcgtgg cgctgggttg gtaccagcag aacgagcccg ccggctggat cgcctccgtg
     1921 atcggagccg tggtgctgct gttcatctac agccttgtca aggggaagtc tcgcgacaac
     1981 gtttgagcgc gagtccgacc ataggaaagc cgcggtttag accggccgcg acggcagcac
     2041 gaccggccaa agaaaaatgg ttagctgcta cttgccggct aaccattgtg cgatctggtg
     2101 gcgcttcacg gactcgaa
//
LOCUS       DASWIY010000376         3396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2127423, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000376 DASWIY010000000
VERSION     DASWIY010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2127423"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VGA59_13470"
     CDS             <1..332
                     /locus_tag="VGA59_13470"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463630.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9720729.1"
                     /translation="VSHLKELGMLEAVAYNQVECFEAGVLFARNEGIVPAPEANHAVK
                     GAIEEALRCKREGKAETILFNLCGHGHFDMLAYSKYFAGELSDEKYDENELAMALAGL
                     PSVPEPA"
     gene            complement(523..>3396)
                     /gene="mfd"
                     /locus_tag="VGA59_13475"
     CDS             complement(523..>3396)
                     /gene="mfd"
                     /locus_tag="VGA59_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX9720730.1"
                     /translation="PTRSRIYKDIGNGVATAGIEYYLPLFFDDTATVFDYVGPGATVV
                     LHGDLERAFQQFWQDTRDRYRLVRGDPERPALPPETLFLTAEQFYGRANEHAQLAVKG
                     FVVPAPDQVRGFSTRDPSVPEVAPLHAGDGLRVKPAMTEAEEASPYAEFEALPPMAVV
                     RGAEEPLAKFKEHLRNTQHRVLLLAESDGRRESLLDFLRASHLAPPAFDSLAQFQASD
                     EKLGIATAPLNTGFSWFEEGLDFITETELFAAGPTTRRRKKQEQVSDVEALIKDLSEL
                     NVGDPVVHAQHGIGRYKGLVNLDLGQGVDEDGSPLMQEFLHLEYADKATLYVPVSQLH
                     QISRYTGVSADEAPLHKLGSGQWEKAKRKAAEQVRDTAAELLNIYARRAAREGHAFRF
                     SAQDYEAFANDFGFEETADQNGAIHAVIQDMVSPRPMDRLVCGDVGFGKTEVALRAAF
                     VAVTGGRQVAFLAPTTLLAEQHYQTLVDRFAKWPVKIAEVSRFRSGKEVSASLKGLAD
                     GTVDIVVGTHKLLSEATKFKNLGLLIIDEEHRFGVRHKETMKAMRAEVDVLTLTATPI
                     PRTLGMALEGLRDLSVIATAPQRRLAIKTFVRNEGNGVIREAVLRELKRGGQVYFLHN
                     EVETIQNRRERLEQLLPEAHIAVAHGQMPERQLERVMRDFVAQRHNLLLCSTIIETGI
                     DVPTANTIVISRADKFGLAQLHQLRGRVGRSHHQAYAYLMVPDTESLTRQAAQRLDAI
                     QQMEELGSGFYLAMHDLEIRGAGEVLGESQSGNMLEVGFQLYNEMLAEAVRSLKAGKE
                     PDLLAPLSVTTEINLHAPALLPDSYCGDVHLRLSFYKRLATARTTDQIDGLLEEIVDR
                     FGKLPPQAQTLVDVHRLRVLAKPYGVVKVDAAPGVIHITFRPKPPIDSMRIIELVQKN
                     RHIKLAGNEKLRIERALKDPKDRAQMVRDVLRSLGQPKVAEPA"
BASE COUNT          533 a         1124 c         1126 g          613 t
ORIGIN      
        1 tggtttcgca cctcaaggag ttgggcatgc tcgaagccgt ggcctataac caggtcgagt
       61 gcttcgaggc cggcgtgctg ttcgcgcgca acgaggggat cgttcccgcg cccgaggcga
      121 accatgccgt caaaggcgcg atcgaagagg cgctgcgctg caagcgggag ggcaaggccg
      181 aaaccattct tttcaacctt tgcggccacg gccacttcga catgctggcg tattccaagt
      241 actttgccgg cgagctgagc gacgaaaagt acgacgagaa cgaactcgcg atggcgctcg
      301 cgggcctgcc gagcgtgcca gagcctgcct gagggagggc ctgagggagc aggcattctg
      361 gattgcgggt caagcccgca atgacagctt cagggctgtc atcgcggacc ccggatcaag
      421 tccggggcag gcccgatccg ggatccagga aattctgaat tgcggtcagg cccgcaatga
      481 cagctcgcta atgtcagctt cgatacagcg caatcaccca acctacgccg gctcggccac
      541 cttcggctgc ccgagcgaac gcagcacgtc gcgcaccatc tgcgcgcggt ccttcgggtc
      601 cttcagggcc cgctcgatgc gcagcttctc attgcccgcc agcttgatgt ggcggttctt
      661 ctgcaccagc tcgatgatgc gcatcgaatc gatcggcggc ttcggccgga aggtgatgtg
      721 gatgacgccg ggcgcggcat cgaccttcac cacgccatag ggcttggcca gcacccgcag
      781 ccggtgcacg tccaccagcg tctgcgcctg cggcggcagc ttgccgaagc ggtccacgat
      841 ctcttccagc aggccgtcga tctggtcggt cgtcctggcc gtggcgagcc gcttgtagaa
      901 ggacaggcgc aggtgcacgt cgccgcagta gctgtcgggc agcagagccg gtgcgtgcag
      961 gttgatctcg gtggtgacgg acagcggcgc cagcaggtcc ggctccttgc cggccttgag
     1021 cgaacgcacg gcctcggcca gcatttcgtt atagagctgg aaacccactt cgagcatgtt
     1081 gccgctctgg ctttcgccca gcacctcgcc ggcgccgcgg atctccaggt cgtgcatcgc
     1141 caggtagaag cccgagccca gttcttccat ctgctggatg gcgtcgagcc gctgcgccgc
     1201 ctgccgcgtc aggctctccg tgtccggcac catcaggtag gcgtaggcct ggtggtggct
     1261 gcgaccgacc cgcccgcgca gctggtgcag ctgcgccaac ccgaacttgt cggcgcggct
     1321 gatgacgatg gtgttggccg tgggcacgtc gatgccggtc tcgatgatgg tggagcacag
     1381 cagcaggttg tggcgctggg ccacgaagtc gcgcatcacg cgctccagtt ggcgctcggg
     1441 catctgcccg tgggccacgg cgatatgtgc ctcgggcagc aactgctcca ggcgctcgcg
     1501 ccggttctgg atggtctcga cttcgttgtg caggaagtag acctggccgc cgcgcttcaa
     1561 ctcgcgcagc accgcctcgc gaatcacgcc attgccctcg ttgcggacaa aggtcttgat
     1621 cgccagccgc cgctgcggcg cggtcgcgat caccgacagg tcgcgcaggc cctccagcgc
     1681 catgcccagc gtgcgcggga tcggcgtggc ggtgagcgtg agcacgtcga cctccgcgcg
     1741 catggccttc atggtctcct tgtggcgcac accgaagcgg tgctcctcgt cgatgatcag
     1801 cagcccgagg ttcttgaact tggtggcctc gctcagcagc ttgtgggtgc ccacgacgat
     1861 gtcgacggtg ccgtcggcca ggcccttgag cgaagcggag acctccttgc cggagcggaa
     1921 gcgcgatacc tccgcgatct tcaccggcca cttcgcgaac cggtccacca gggtctggta
     1981 atgctgctcg gccagcagcg tggtcggcgc caggaaggcc acttgcctgc cgcccgtcac
     2041 cgcgacgaag gccgcgcgca gtgcgacctc ggtcttgccg aagcccacgt cgccgcaaac
     2101 cagccggtcc atcggacggg gcgagaccat gtcctggatc accgcgtgga tcgcaccgtt
     2161 ctggtccgcg gtctcctcga aaccgaagtc gttggcgaag gcttcgtaat cctgggccga
     2221 gaagcggaac gcatggcctt cgcgcgcggc gcggcgggcg tagatattga gcagttccgc
     2281 cgcggtgtcg cgcacctgtt ccgcggcctt tcgcttcgcc ttttcccatt ggccggagcc
     2341 gagcttgtgc aacggcgctt catcggccga aacgccggtg tagcggctga tctggtgcag
     2401 ctggctcacc ggcacgtaca gggtcgcctt gtccgcgtat tcgaggtgca ggaattcctg
     2461 catcagcggc gagccgtcct cgtccacgcc ctggccgagg tccaggttca ccaggccctt
     2521 gtagcgccct atgccgtgct gcgcgtggac caccgggtcg ccgacgttca gttccgacag
     2581 gtccttgatc agcgcctcga cgtcgctgac ctgttcctgc ttcttgcgcc ggcgcgtggt
     2641 cggaccggcc gcgaacagct cggtttcggt gatgaagtcg aggccttcct cgaaccagct
     2701 gaatcccgtg ttcagcggtg cggtcgcgat gcccagcttt tcgtcgctcg cctggaactg
     2761 cgccagcgaa tcgaaagccg gcggggcgag atggctggcc cgcaagaaat ccagcaggct
     2821 ctcgcggcgg ccatcgcttt cggccagcag gagcacgcga tgctgcgtgt tgcgcagatg
     2881 ctccttgaac ttcgcgagcg gttcctcggc gccccgcaca acggccatcg gcgggagggc
     2941 ttcgaattcg gcgtaaggac tggcttcctc cgcctctgtc attgcgggct tgacccgcaa
     3001 tccatccccc gcgtgaagag gcgcaacttc aggcacggat ggatcccggg tgctgaaacc
     3061 ccggacttga tccggggccg ggacgacaaa tcccttcacc gccagctgcg cgtgctcgtt
     3121 cgcgcggccg tagaactgct cggccgtgag gaacaaggtt tccgggggca gggccggccg
     3181 ttcggggtcg ccacgcacca gccgatagcg gtcccgcgtg tcctgccaga actgctggaa
     3241 ggcccgctcg aggtcgccgt gcagcaccac cgtggcgccg gggccgacgt aatcgaatac
     3301 cgtcgccgtg tcatcgaaga acagcggaag gtagtactcg atgccggcgg tggccacgcc
     3361 gttgccgatg tccttgtaga tgcggctcct ggtcgg
//
LOCUS       DASWIY010000377         7745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1006534, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000377 DASWIY010000000
VERSION     DASWIY010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006534"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..939)
                     /gene="urtD"
                     /locus_tag="VGA59_13480"
     CDS             complement(64..939)
                     /gene="urtD"
                     /locus_tag="VGA59_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEX9720731.1"
                     /translation="MTPDLLEDGARRAQAHRERTLRGRTESGGRQAGFGRPAVAGEVD
                     VTHGRILYLEGVSVSFDGFKAIDKLSLDIAPGELRCVIGPNGAGKTTMMDIITGKTRP
                     DEGTVFFGSTIDLLRYTEPQIAQMGIGRKFQKPTVFEQLTVFENLELALKTGKGVKSS
                     MFFRLDSGQGDRLAEVLHTIHLAQGLTRLAGTLSHGQKQWLEIGMLLMQDPKLLLLDE
                     PVAGMTDEETARTAELFLDLKGRHSLMVVEHDMSFIKSISEIVTVLSEGSVLAQGTLE
                     QVQNDERVIEVYLGR"
     gene            complement(936..2057)
                     /gene="urtC"
                     /locus_tag="VGA59_13485"
     CDS             complement(936..2057)
                     /gene="urtC"
                     /locus_tag="VGA59_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEX9720732.1"
                     /translation="MLPSREPVLGRAGWSAFIVALIAVCAVAPLLNLVLPQGNVFHMS
                     DYAVALVGKIMCYAILALAMDLIWGYTGILSLGHGLFFALGGYAMGMYLMRQIGRDGN
                     YKSDLPDFMVFLDWKALPWHWSFSDSFIATLFLIVAVPGLVAFVFGYFAFRSRIKGVY
                     FSIITQAMTFAAMLLFFRNETGFGGNNGFTDFKRILGIPIATPSMRMVLFVLTGVALL
                     GFFLFGRWLVAAKFGRVLQAVRDAESRVMFSGYNPIGYKLTIWTISAVMCGVAGALYV
                     PQVGIINPSEMSPANSIEIAIWAAVGGRATLIGPIIGAFLVSGAKSWLTVAAPEFWLY
                     FLGALFIGVTLFLPDGVLGLLRKSGGVAAWARRLVGPRP"
     gene            complement(2198..3769)
                     /gene="urtB"
                     /locus_tag="VGA59_13490"
     CDS             complement(2198..3769)
                     /gene="urtB"
                     /locus_tag="VGA59_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEX9720733.1"
                     /translation="MLIRSLFLLVAFLGGQAHALTAAEARAMASGETDARIEAVNKAI
                     AAGDDRTAAFIQALADDAVKAAGDKVLVIKDGKAFDPVTGSETALPADAEDVINNNRM
                     RGELDAAMAALKLFSKDVVQRREAIKTLENESDESKLPLIEKAHASETDSQVKEQLGM
                     VRAAVLLGSSDKAKRLEAAKLLAQSSNPGTKTLLIERLKVEAEDDVKSALSASLRSVE
                     ASLAWGDKAGALFSGISLGSILLLVALGLAITYGLMGVINMAHGELMMLGAYATYVVQ
                     GLFQKYLPASFDWYLVAAVPAAFATSALMGAALERSVIRFLYGRPLETLLATWGISLM
                     LMQLVRSVFGAQNVGVENPSWMSGGVVVMGNLQLPWNRIVIVGFALAVLLGMAFLIGR
                     TRLGLFVRGVTQNRPIASCMGVNTARIDTYAFALGSGIAGLAGCALSQIGNVGPDLGQ
                     GYIVDSFMVVVLGGVGQLAGTVYAALGLGILNKLLEGWAGAVLAKIAVLVFIIVFIQK
                     RPQGLFAMKGRSAEA"
     gene            complement(3973..5241)
                     /gene="urtA"
                     /locus_tag="VGA59_13495"
     CDS             complement(3973..5241)
                     /gene="urtA"
                     /locus_tag="VGA59_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEX9720734.1"
                     /translation="MQRRFTLKALTAAIALAGLTVLPAHAQSKGTIKVGILHSLSGTM
                     AISETVLKDTVLMAIDEINAKGGVMGKKLEPVVVDPASNWPLFAEKTKQLLTQDKVAV
                     IFGCWTSVSRKSVLPVVEELNGLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVDYLM
                     SKDGGGAKRWVLLGTDYVYPRTTNKILRAYLKSKGVKDTDIDEKYTPFGHSDYQTIVA
                     DIKKFSAGGKTAVVSTINGDSNVPFYKELGNAGLKAKDVPVVAFSVGEEELRGVDTKP
                     LVGHLAAWNYFQSIKNPTNTEFSKKWAAYAKAKNLPGHKDKPLTNDPMEATYIGINMW
                     KQAVEKAKSTDVDKVIAAMAGQTFKAPSGIVSKMDEKNHHLHKSVFIGEVKADGQFNV
                     VWKTPGPVKAKPWSPYIEGNDKKPDEPAKK"
     gene            5506..6126
                     /locus_tag="VGA59_13500"
     CDS             5506..6126
                     /locus_tag="VGA59_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9720735.1"
                     /translation="MTSTSLLTRLSPSITNMIRLDHTHVMSTFHQYEVDTPPRIKKGL
                     ADTVCVSLEIHAQLEEEIFYPALRAVAQTEVVQKSTTEHHQMRQLINQLRNLEPYDAA
                     FDQTFFQLMNTLMHHVADEETLLLPAAERVLADRLGELGAEMTKRRMQLAVPRTGQIA
                     GGMARSMSTGALLAAAGTLLAGGYLMTRPADKRWQWGRRSTSEPIR"
     gene            complement(6356..7531)
                     /locus_tag="VGA59_13505"
     CDS             complement(6356..7531)
                     /locus_tag="VGA59_13505"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX9720736.1"
                     /translation="MEQTAEQAQEEVIGLGEDWRVVQALLPERWKEMARELGAFQRAR
                     GIGDARALLQVMLIHLGEGCALRETAVRASLAGIAHVSDVALLKRLRSCGPWFEWLAH
                     ELRAGQPDEGTQGLLGGRRLRVVDGSVVREPGVTGSQWRLHYAIGLPSLQCQEVHLGP
                     CDEGETLKRFQVHAGDIFVADRGYAHPGGIAHVRRHDGDVIVRMNLVTLPLTHPSTGK
                     PLDVLQQVRGLQVGQAGSWPACLKVKAERGGQRAQEIVGRLCAVRKSAAAAAVGRDRV
                     RRESMRGRRQLQPQTLKAAEYVLIFTTLGPDISAEQVMTLYRLRWQIELEFKRLKSLL
                     QLGHLKKHDARAARSWLQGKLLVAVLIARLIAHSERVSPWGYEFAWFDSSARALPVA"
     gene            complement(7665..>7745)
                     /locus_tag="VGA59_13510"
     CDS             complement(7665..>7745)
                     /locus_tag="VGA59_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically inducible protein C"
                     /protein_id="HEX9720737.1"
                     /translation="HDKYCSASVMLGKTAEITTSFELVEV"
BASE COUNT         1366 a         2603 c         2430 g         1346 t
ORIGIN      
        1 ccgcggctcc gaacctgcct gcgctggcag tggcacgtcg catcggcgct tcgggggtgg
       61 gctttacctt ccaagataca cctcgatcac ccgctcgtcg ttctgaacct gctccagggt
      121 gccctgcgcc agcaccgaac cttcggacag caccgtgacg atctccgaga tgctcttgat
      181 gaaactcatg tcgtgctcca ccaccatcag cgaatgccgg cccttgagat cgaggaagag
      241 ttccgccgtg cgcgccgttt cctcatcggt cattccggcc accggctcgt ccagcagcag
      301 cagcttgggg tcctgcatca acagcatgcc gatctcgagc cactgtttct ggccgtggct
      361 caaggttccg gccaggcgcg tcaggccctg ggcgagatgg atggtgtgca gcacctcggc
      421 cagccggtca ccctgccccg aatcgaggcg gaagaacatg gacgacttca cgcccttgcc
      481 ggtcttgagc gccaactcca ggttctcgaa caccgtcaac tgctcgaaca cggtgggctt
      541 ctggaacttg cggccgatgc ccatctgggc gatctggggc tccgtatagc gcaggaggtc
      601 gatcgtgctg ccgaagaaga ccgtgccttc gtcgggccgg gtcttgccgg tgatgatgtc
      661 catcatcgtg gtcttgcccg cgccgttcgg gccgatgacg cagcgcagct cgccgggcgc
      721 gatgtccagc gacagcttgt cgatggcctt gaagccgtcg aagctcacgc tcacgccctc
      781 caggtacagg atgcgtccgt gggtgacgtc cacctcgccc gcgacggccg gccggccgaa
      841 gcccgcctga cggccgccgg actccgtcct gccgcgcaac gtgcgctcgc ggtgagcctg
      901 ggcgcggcgc gcgccgtcct cgagcaggtc cggcgtcatg ggcgagggcc cactagacgc
      961 ctggcccatg cggcaacgcc gcccgatttg cgcagcaggc ccagcacgcc atcgggaagg
     1021 aacagcgtga ccccgatgaa cagggcgccc agaaagtaca gccagaattc cggcgccgcc
     1081 accgtgagcc agctcttggc accgctcacc aggaaagcac cgatgatggg cccgatcagg
     1141 gtggctcgcc cgccgaccgc agcccagatc gcgatctcga tggagttggc ggggctcatc
     1201 tcgcttgggt tgatgatgcc gacctgcggc acatagagtg cgcccgccac gccgcacatg
     1261 accgcggaaa tcgtccagat cgtgagcttg tagccgatcg ggttgtagcc cgagaacatg
     1321 acgcgggact cggcgtcgcg gaccgcctgc agcacccggc cgaacttggc cgcgaccagc
     1381 cagcgcccga acaggaaaaa gcccagcagg gcgacgccgg tgagcacgaa cagcaccatg
     1441 cgcatcgaag gcgtcgcgat cgggatgccg aggatgcgct tgaaatcggt gaagccgttg
     1501 ttgccgccga agccggtctc gttgcggaag aacagcagca tcgcggcgaa cgtcatcgcc
     1561 tgcgtgatga tggagaagta cacgcccttg atgcgcgagc gaaaagcgaa atagccgaac
     1621 acgaaggcca ccaggccggg cacggcgacg atcaggaaca aggtggcgat gaagctgtcg
     1681 ctgaagctcc agtgccacgg caaggccttc cagtccaaaa acaccatgaa gtccggcagg
     1741 tcgctcttgt agttgccgtc gcggccgatc tggcgcatca ggtacatgcc catggcatag
     1801 ccgcccagcg cgaagaacag gccatggccc agcgacagga tgccggtgta accccagatc
     1861 aggtccatgg ccagcgcgag gatcgcgtag cacatgatct tgcccaccag cgcgacggcg
     1921 tagtcgctca tgtggaagac gttgccctgc ggcaagacca ggttcagcag gggagcaacc
     1981 gcgcacacgg cgatgagcgc gacgatgaac gcgctccagc cggcgcggcc gaggacgggc
     2041 tcacggctcg gcagcacgaa tgtggctccc cctcccgctg gcgggagagg attggggtga
     2101 gggtagctcg cggctgcaag tccctcatcc cccgccttct cccgcgcgcg ggacaaggcg
     2161 ccatgctcca gggtctcctc tggtggcagc accgcgctca tgcctccgcg ctccggccct
     2221 tcatcgcaaa aagtccttgc ggccgcttct ggatgaacac gatgatgaat acgagcaccg
     2281 cgatcttggc cagcacggcg ccggcccagc cttcgagcaa cttgttgagg atgcccaggc
     2341 cgagcgcggc gtacacggtg ccggcgagct gcccaacgcc gcccagcacc accaccatga
     2401 acgagtccac gatgtagccc tggcccaggt cggggcccac attgccaatc tggctcaggg
     2461 cgcaaccggc cagccccgcg atgcccgagc cgagcgcgaa cgcgtacgtg tcaatgcgcg
     2521 ccgtattcac acccatgcaa gatgcgatcg ggcggttctg cgtgaccccg cgcacgaaca
     2581 ggcccagccg cgtgcgtccg atcaggaagg ccatgcccag cagcaccgcc agcgcgaaac
     2641 ccacgatgac gatgcggttc cacggcaatt gcagattgcc catcaccacc acgccgccgc
     2701 tcatccagct ggggttttcc acgccgacgt tctgggcgcc gaagaccgac cgcaccagct
     2761 gcatcagcat caggctgatg ccccaggtgg ccagcagcgt ttccagcggc cggccgtaca
     2821 ggaaacggat cacgctgcgc tccagcgccg cacccatgag cgcggaggtt gcaaaggctg
     2881 ccggcacggc ggcgaccagg taccagtcga aggatgcggg caggtatttc tggaacaggc
     2941 cctgcaccac ataggtggca taggcgccca gcatcatcag ctcgccatgg gccatgttga
     3001 tcacgcccat gaggccgtag gtgatggcca gccccagcgc caccagcagc aggatggacc
     3061 ccaggctgat gcccgagaac aacgcgccgg ccttgtcgcc ccaggcgagc gaggcttcca
     3121 cgctgcgcag gctcgcggac agggcggatt tcacgtcgtc ctcggcctcc accttgagcc
     3181 gctcgatcag cagcgtcttc gtgcccgggt tgctgctttg cgccagcagc ttggcagctt
     3241 ccaggcgttt ggccttgtcg gagctgccca gcagcacggc cgcgcgcacc atgcccaatt
     3301 gctccttcac ctgggaatcc gtttccgagg cgtgggcttt ttcgatcagc ggcagcttgg
     3361 actcatcgct ttcgttttcc agcgtcttga tcgcctcgcg ccgctgcacg acgtccttcg
     3421 agaacagctt gagcgcggcc atggcggcat cgagctcgcc gcgcatgcgg ttgttgttga
     3481 tcacgtcctc cgcgtcggcc ggcagcgccg tctcgcttcc cgtcaccggg tcgaaggcct
     3541 tgccgtcctt gatcacaagc accttgtcgc cggcggcctt cacggcgtcg tcggccagcg
     3601 cctggataaa ggcggcagtc ctgtcatcgc ctgcggcgat cgccttgttg acggcttcga
     3661 tacgggcatc ggtctcgcca gacgccatcg cgcgcgcctc ggcggcggtg agcgcatggg
     3721 cttgaccgcc gagaaaggca acgagaagga agagactgcg aatcagcatt gaaagacctt
     3781 gcccctgact tgtcattgcg ggcttgaccc gcaatccatg cctcggccga agagggacat
     3841 ggatcccggg tcaagcccgg gatgacagac tgtgcactgg atcccgggtc aagcccggga
     3901 tgacagacag cgccctggat ccccgccttc gcggggatga cgcagctcat ccggcttcgc
     3961 cggatcgctg cgtcacttct tcgcgggctc gtccggcttc ttgtcgttgc cttcgatgta
     4021 cggcgaccag ggcttggcct tcaccgggcc gggcgtcttc cacaccacgt tgaactgtcc
     4081 gtcggccttg acttcgccga tgaacaccga cttgtgcagg tggtggttct tctcgtccat
     4141 cttggagacg atgccgctcg gcgccttgaa agtctggccg gccatggcgg cgatgacctt
     4201 gtcgacgtcg gtggacttgg ccttctcgac ggcctgcttc cacatgttga taccgatgta
     4261 ggtggcctcc atcgggtcgt tggtcagggg cttgtccttg tggcctggga ggttcttggc
     4321 cttggcgtag gcagcccact tcttggaaaa ctcggtgttg gtcgggttct tgatcgactg
     4381 gaagtagttc caggcggcca ggtggcccac cagcggcttg gtgtccacgc cgcgcagctc
     4441 ttcctcaccc acggagaagg cgacgaccgg cacgtccttg gccttcaggc cggcgttgcc
     4501 cagttccttg tagaacggca cgttggagtc gccgttgatg gtggacacca cggccgtctt
     4561 gccgccggcc gagaatttct tgatgtcggc gacgatggtc tggtagtcgc tgtggccgaa
     4621 gggggtgtac ttctcgtcga tgtcggtatc cttcacgccc ttgctcttca ggtaggcgcg
     4681 caggatcttg ttggtggtgc gcgggtacac gtaatcggtg cccagcagca cccatcgctt
     4741 ggcgccgccg ccgtccttgc tcatcaagta gtccaccgcg gggatggctt gctggttggg
     4801 ggccgcgccc gtgtagaaca cgttcttgga cagctcctcg ccctcgtact gcaccggata
     4861 gaacagcagg ccgttgagtt cttccacgac cggcagcacc gacttgcgcg acaccgaggt
     4921 ccagcagccg aagatcacgg ccactttgtc ctgcgtcagc agctgcttgg tcttttcggc
     4981 gaacagcggc cagttggaag ccgggtccac caccaccggc tccagcttct tgcccatcac
     5041 gccgcccttg gcgttgatct cgtcgatggc catcagcacg gtgtctttca gcacggtctc
     5101 ggagatggcc atggtgcccg acagactgtg caggatgccg actttgatcg tgcccttgga
     5161 ctgggcatgg gccggcagga ccgtgaggcc cgccagcgcg attgcggcgg tgagcgcctt
     5221 gagggtgaaa cgacgttgca tgtgcgtgtg ctcctagcga agtggaaacc tgcgaaaaga
     5281 aaaggcctgg aattcgcccc accttgcccc cgaggggttg ccgatgtgga cacacggatg
     5341 ctaggagcgc cctcgcgggc acggaatacg gcacatggcg tacgctgcgc acacggaacc
     5401 gggtcgggac ggaacggtag gccggacaag gcgccgtccg acaagcagga cagcgcggac
     5461 gcggccaaat gacgcagccc ccttcggcgt ttcgaggaaa actccatgac ttcaacttca
     5521 ctcctgaccc gtctgtcgcc gagcatcacg aacatgatcc gcctggacca cacccatgtc
     5581 atgtcgactt tccatcagta cgaggtcgac acgccgccgc gcatcaagaa ggggctggcc
     5641 gacacggtgt gcgtatcgct cgagatccat gcccagctgg aagaagagat cttctatccg
     5701 gccctgcgcg cggtggcgca gaccgaggtg gtgcagaaga gcacgacgga gcaccaccag
     5761 atgcgccagc tcatcaacca gctgcgcaat ctggagcctt atgacgccgc cttcgatcag
     5821 acttttttcc agctcatgaa cacgctcatg caccatgtgg ccgacgagga gaccctgctg
     5881 ttgccggcgg ccgagcgggt gctggcggac cggctgggcg aactcggcgc cgagatgacc
     5941 aagcggcgca tgcagttggc cgtgccccgc accggtcaga tcgccggcgg catggcccgc
     6001 tcgatgagca caggcgcctt gctggcggcg gccgggacgc tgctggccgg cggctacctg
     6061 atgacgcggc ccgccgacaa gcgctggcag tggggacgcc ggtccacgtc ggagccgatt
     6121 cgctgagtat tgtcttgtca ttgcaggctg ggctcgcaat ccaggaagtc atggatcccg
     6181 ggtcaagccc cataagcgct aacttatagg ctcacgtgct cttttccgct ggagaaccct
     6241 cttgcgtggg ggctctgcga ggcggtgtcg caatgcttgc cactgcgcaa tggcgtcaac
     6301 aagactgagg acgggcgcca cgccacggca aagcagttca agcatcagcg agatttcacg
     6361 ccacgggcag tgctctcgcg gacgagtcga accatgcaaa ctcgtatccc cagggggaaa
     6421 cgcgctcgga gtgagcgatc aggcgagcaa tcaacacggc caccagaagc tttccctgca
     6481 gccaacttcg cgccgcccgc gcatcgtgct ttttcaggtg gcccagctgc aggagcgact
     6541 tcagccgctt gaattccagc tcgatctgcc agcgcagccg atacagtgtc atgacttgtt
     6601 cggcgctgat atcgggcccg agcgtggtga agatcagcac gtactcggcc gccttcagcg
     6661 tctgaggctg cagttgccgg cgcccgcgca tgctctcgcg gcgcacccga tcacgcccca
     6721 ccgcagcggc cgcagcgctc ttgcgcaccg cgcacagccg ccccacgatc tcctgcgcgc
     6781 gctgaccgcc gcgttcggcc ttgaccttca ggcaggcagg ccagctgccc gcctgcccca
     6841 cctgcagtcc tcgcacctgc tgcagcacgt caagtggttt gcccgtactg gggtgcgtca
     6901 gcggcagggt caccaggttc atgcgcacga tcacatcgcc gtcatggcgc ctgacatggg
     6961 caattcctcc ggggtgcgca tagccgcggt cggccacaaa gatgtcgccc gcgtgcacct
     7021 ggaagcgctt gagggtttcc ccctcgtcgc aagggcccag gtgcacttcc tggcactgca
     7081 ggctcggcag cccaatggcg tagtgcagcc gccactgcga tccggtcacg ccaggctcgc
     7141 gcacaacgct gccatctacg acgcgcagcc gtcgcccgcc cagcagccct tgcgtcccct
     7201 catcgggctg cccggcgcgc agctcgtgcg ccagccactc gaaccacgga ccgcaggacc
     7261 gcagtctctt gagcagagcc acgtcgctga cgtgcgcgat gccggcaagg gacgcccgca
     7321 cggccgtctc gcgaagcgcg cagccttcgc ccaggtggat cagcatgact tgcagcagcg
     7381 cccgcgcgtc gccaatgccg cgagcgcgct ggaacgctcc caactcgcgc gccatctcct
     7441 tccatcgctc aggcagcagc gcctgcacga cgcgccaatc ctcacccagc ccgatcacct
     7501 cttcctgggc ctgctctgcg gtttgctcca tgggacaccc tccggctcag ctgttaccag
     7561 ccgaaggata tctcaaaagt aaacgattaa gttagcgctt atggggtcaa gcccgggatg
     7621 acaacttggt caagcccggg atgacaagtt cacggacagc ggcactagac ttcgacgagt
     7681 tcgaagctcg tcgtgatctc ggccgtcttg cccagcatga cgctggccga gcagtacttg
     7741 tcgtg
//
LOCUS       DASWIY010000378         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_630695, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000378 DASWIY010000000
VERSION     DASWIY010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630695"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /gene="nusG"
                     /locus_tag="VGA59_13515"
     CDS             <1..385
                     /gene="nusG"
                     /locus_tag="VGA59_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX9720738.1"
                     /translation="GQKHMSERKFFPGYVLVEMEMDDDTWHLVKNTNKVTGFVGGSAM
                     RPTPISEKEVNAIMQQMREGVEKPKPKVLFENGEMVRVKDGPFTDFQGTVEDVNYEKS
                     RLRVAVTIFGRSTPVELEFGQVEKA"
     gene            448..879
                     /gene="rplK"
                     /locus_tag="VGA59_13520"
     CDS             448..879
                     /gene="rplK"
                     /locus_tag="VGA59_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX9720739.1"
                     /translation="MAKKVVGFIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQKVEPGLPLPVVITAYADKSFSFIIKTPPTTVLIKKAAGIESGSPRPHTDKVGKIT
                     RKQVEEIARAKTPDLTAGDLEAAMRTVAGSARSMGILVEGL"
     gene            880..1578
                     /gene="rplA"
                     /locus_tag="VGA59_13525"
     CDS             880..1578
                     /gene="rplA"
                     /locus_tag="VGA59_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX9720740.1"
                     /translation="MAAISKRRKAIVSKADHDKAYAVIDAMKIIKETATAKFDEAVDV
                     AVNLGIDTSKSDQTVRGAVVLPAGTGKTVRIAVFAQGDKAQAAKAAGADVVGMEDLAE
                     SVKAGKLDFDVVIAEPAAMRVVGQLGQILGPRGLMPNPKVGTVTPDVAAAVKNAKAGQ
                     VQFRADKTGIVQCTIGRASFTPEQLRDNLAALVDALNKSKPQHTKGVFLRKLSVSSTM
                     GIGVRVDPATLQAQ"
     gene            1778..>2227
                     /gene="rplJ"
                     /locus_tag="VGA59_13530"
     CDS             1778..>2227
                     /gene="rplJ"
                     /locus_tag="VGA59_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX9720741.1"
                     /translation="MSLSLEQKQAVVAEIASQLAKAQAVIVAENRGLSVEAVTSLRAK
                     ARKSGLYLRVLKNTLARRAVKGTPFEALAGQLSGPLVYGIAQDPVSGAKVLAEFAKEN
                     GLFVIKGGAMPNAMMSSTDVKALATMPSREELLAKLAGTLQAPIAKLA"
BASE COUNT          459 a          644 c          773 g          351 t
ORIGIN      
        1 cggccagaag cacatgtcgg agcgcaagtt cttccccggc tacgtgctgg tggaaatgga
       61 gatggacgac gacacctggc acctggttaa gaacaccaac aaggtgaccg gcttcgtcgg
      121 cggctcggcc atgcggccga cccccatttc cgagaaggaa gtcaacgcca tcatgcagca
      181 gatgcgtgag ggcgtggaga agcccaagcc caaagtgctg ttcgagaacg gcgagatggt
      241 gcgcgtgaag gacggcccgt ttaccgattt ccagggcacg gtggaggacg tcaactacga
      301 gaagagccgc ctgcgcgtgg cggttacgat tttcggacgc tcgactccgg tcgagctgga
      361 gttcggccag gtcgagaagg cctgagtccg agtcacttgc acggggagag cgctgcggcg
      421 ttcgcttgca cccgatagga gccagacatg gcgaagaaag tagtgggatt catcaagctg
      481 caggtgccgg cgggcaaggc caacccctcg ccgccgatcg gtccggcgct cggtcagcgc
      541 ggcttgaaca tcatggagtt ctgcaaggcg ttcaacgcgc aaacgcagaa ggtggagccg
      601 ggtttgccgc tgccggtggt cattacggcg tatgcggaca agagtttcag cttcatcatc
      661 aagacgccgc cgaccacggt gctcatcaag aaggccgccg gcatcgaatc cggcagtccg
      721 cggccgcata ccgacaaggt gggcaagatc acgcgcaagc aagtcgagga gatcgccagg
      781 gcgaagacgc cggacctgac ggcgggcgat ctggaagcgg cgatgcgcac cgtggccggt
      841 agtgcgcgct cgatgggcat actggtggag ggcctgtaaa tggcggctat ttccaaacgc
      901 cggaaggcga tcgtcagcaa ggccgaccac gacaaggcct acgcggtcat cgatgccatg
      961 aagatcatca aggagaccgc gaccgccaag tttgacgaag ccgtggacgt ggcggtgaac
     1021 cttggcatcg acacttcaaa atccgaccag acggtgcgcg gtgcggtggt attgcctgcc
     1081 ggcaccggca agacagtacg catcgcagtg tttgcgcagg gcgacaaggc ccaggccgcc
     1141 aaggcggcag gcgccgacgt ggtcgggatg gaggacctcg ccgagagcgt caaggctggg
     1201 aagctcgatt tcgacgtggt catcgccgaa ccggcagcaa tgcgggtggt cggccagctc
     1261 ggccagattc tcggcccgcg cggcctgatg ccgaatccga aagtgggtac ggtgacgccg
     1321 gatgttgcgg cagcggtgaa aaacgccaag gcgggccagg tgcagtttcg cgccgacaag
     1381 accggaatcg tgcaatgcac gatcggccgc gcctcgttca cgcccgaaca gttgcgcgac
     1441 aaccttgcgg cgctggtgga cgcattgaat aagtcgaagc cgcagcacac caagggcgtg
     1501 ttcctgagga agctctcggt gtcgagcacg atggggatcg gtgtgcgcgt ggatccggca
     1561 acattgcagg cgcagtgagg aacgatggaa ctttgggccg ctgttccacg gaacagcagc
     1621 tgtcaaagac cgtaggggcc cgatgggctt aatccagttg ccgccctacg cagacggcgc
     1681 acccgaagca ggaatgcatt tgcggttcct gattcaaggt cgccagggtg gaggtccgga
     1741 aacggacttc cgtttgttca acgcaaggag gtagaccttg agtctcagtc tggagcagaa
     1801 gcaggcggtc gttgccgaga tcgcgtcgca gctggcgaag gcccaggcgg ttatcgtcgc
     1861 ggaaaatcgc ggcctgagcg tcgaagccgt taccagcctg cgcgcgaagg cgcggaagtc
     1921 gggcctctac ctgcgcgtgc tgaagaacac gctcgcgcgt cgcgccgtca agggcacgcc
     1981 gttcgaggcg ctggccgggc agctgtccgg tccgctggtg tacggcatcg cgcaggaccc
     2041 cgtgtcggga gccaaggtgc tggcggaatt cgccaaggag aacgggcttt tcgtcatcaa
     2101 gggcggggcg atgcccaacg ccatgatgtc ctcgacggac gtcaaggcgc tggccaccat
     2161 gcccagccgc gaagagcttc tggcgaaact tgccggcacg ctgcaggccc ccatcgcgaa
     2221 gctggcg
//
LOCUS       DASWIY010000379         6270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2131522, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000379 DASWIY010000000
VERSION     DASWIY010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131522"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VGA59_13535"
     CDS             <1..1081
                     /locus_tag="VGA59_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9720742.1"
                     /translation="GFMLPYTGTYAQLGVAIENGVRMAIDEKGGKLGGREIDWFKVDD
                     ESEPSKGVENASRLVQRDKVDVLIGTVHSGVQMGIQKVVRDSGVLCLIPNAGVHAATR
                     ALCAPNVFRTSFTNSQPTLALGKVMVDKGHKKAVWITWKYAAGEEAFEGFRDSYTKAG
                     GTIVKELGVPFPNVEFQALLTEIASINPDAVACFFAGGGAAKFIRDYAAAGLKGRIPL
                     YGSGFLTEGVLDAAGPAADGILTTLHYSDALDTPRNKQFRLAYAKAFRSQPDVYAVQG
                     YDTGQLLVQGAAAVRGDLNNKQALYKALESAVIDSPRGKWTMSKAHNPVQDIYLRQVV
                     NKENKVIGIAAKALADSGAGCKMAG"
     gene            1325..2188
                     /locus_tag="VGA59_13540"
     CDS             1325..2188
                     /locus_tag="VGA59_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9720743.1"
                     /translation="MDLANFLIQLLNSVQYGLLLFMLAAGLTLIFGIMGVVNLAHGSF
                     YMLGAYLAWWVSSMTGSFTLAIILGALLSVVFGLVLERLLFRHFYQRDHLDQVLLTFG
                     LIYIFEESRSILWGDDVHGVKIPELLAASIPLTDNLSYPVYRLFISGVCIALAIGLYL
                     LISKTRLGMKIRAGAFNRPMTEALGVNIKLIHAVVFAIGVALATVAGMIASPISSVYP
                     GMGSQVLIISFVVVVIGGIGSVRGALISAMLVGLVDTFGKVLLPAVAGMLIYMLMAAV
                     LLWKPEGLFKQ"
     gene            2277..3272
                     /locus_tag="VGA59_13545"
     CDS             2277..3272
                     /locus_tag="VGA59_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9720744.1"
                     /translation="MTAAANLETLPRSLQIVLALGFLALAAFPFFGTDFYAQMVSRMM
                     ILAIFAMSLDLLLGVTGLVSLGHAAFFGLAGYALAFITPAGEPVSLWWTLPVAVAASG
                     LAALVIGFFVVRTRGIYFIMVTMAFAQMVFYLFFDNKVLGGSDGLYVNFKPNAGLFDL
                     EDKQVFYYFTLAVLLLVYLFLRRLLWSPFGRVLAGIRVNEHRMRAMGYRTFGYKLTAF
                     SLAGALAGLAGYLWGAQTGYINPELMGFHMSAHAIMMVILGGMGNFAGAIVGAFAFEY
                     LLHEFKDLPTIGAVHLGKHWQMWMGLFIVLVVTFAPRGLLGMVERLSRRKERTGD"
     gene            3265..4014
                     /locus_tag="VGA59_13550"
     CDS             3265..4014
                     /locus_tag="VGA59_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720745.1"
                     /translation="MTEPLLSAKQLTKLFGGLAAVNQVSVDLWQGRIHALIGPNGAGK
                     STLTNLLSGDLPPTTGTITLDGRDVTGWTPEKISRVGLGRSYQKTNIFLPFTVWENVR
                     LAAQSRTPHASRWLSRATGFAAINDRANRAIELAGLEDRTHQIAGNISHGEQRQLEIA
                     IALATDPKVLLLDEPLAGMGIAEAQRMVDLLLKLKPAHAIMLVEHDMDAVFALADHLT
                     VMVNGQVIASGKPPDVRADANVQSAYLGEEH"
     gene            4055..4768
                     /locus_tag="VGA59_13555"
     CDS             4055..4768
                     /locus_tag="VGA59_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720746.1"
                     /translation="MSELLIDARALHTYYGASHILRGIDFTVAPGETIGLMGRNGMGK
                     STLLKTLIGHVKPRSGSVRVMGQDMTARPSYEIAQRGVAYVPEGRGIFGNLSVEENLK
                     MAARPGPRGQRDWTYERVLETFPRLGERLRQGGQQLSGGEQQMLTIGRALMTNPDVLI
                     LDEATEGLAPLIARDIWSICSLIRESGISSVIVDKNWKHVTQITDRNVILVKGEIVFA
                     GTSDELRARPQLLEQYLGV"
     gene            complement(4781..5695)
                     /locus_tag="VGA59_13560"
     CDS             complement(4781..5695)
                     /locus_tag="VGA59_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9720747.1"
                     /translation="MQDLNDMFYFAEVVDRGGFAAAGRALGLPKSKLSRRVAELEARL
                     GVRLLQRTTRKLSLTAAGEVFHRHCAAMREEAEAAADAVAAVHNEPRGTIRVACPVTL
                     AQSTVGPLIPLFLERHPHVRIEMQVSNRVVDLVQDGVDVALRVRSTLDDSGSMIVKNL
                     GISQGLVVASPSQLERQGRPETVEDLQRLSTLAMSAPDGRASWLLSGPSAREYEFQHR
                     PCYTADDLLTLRYAVLKGTGMCIMPDYLCRRELQRGELEQVLPGWAPRPGVIHAVFPS
                     RRGLIPAVRHFLDFLGEHVTGEGLSCPD"
     gene            5817..>6270
                     /locus_tag="VGA59_13565"
     CDS             5817..>6270
                     /locus_tag="VGA59_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX9720748.1"
                     /translation="MRLLHIDSSPLAGNSVSRQLTERIVAQWRATHPGTVVEYLDLAA
                     DAPSHLSIDSLGFRAGPDAKGQSEVQRRENAISERLVSQFLAADVIVVGAPMYNFSIP
                     SQLKAWIDRVAQAGRTFTYTEKGPKGLAAGKTVIVASTRGGIYSSNPAM"
BASE COUNT         1074 a         2081 c         1972 g         1143 t
ORIGIN      
        1 gggtttcatg ctgccttaca caggcaccta cgcccagctt ggcgtggcca ttgaaaacgg
       61 cgtgcgcatg gccatcgacg aaaaaggcgg caagctgggc ggccgcgaga tcgattggtt
      121 caaggtggac gacgagtccg agccttcgaa aggcgtggaa aacgccagcc ggctggtgca
      181 gcgcgacaag gtcgacgtgc tgatcggcac cgtgcactcg ggcgtgcaga tggggatcca
      241 gaaagtggtg cgcgacagcg gcgtgctgtg cctgatcccc aatgccggtg tgcatgcagc
      301 cactcgcgcc ctgtgcgccc cgaatgtctt tcgcacgtcg ttcaccaact cccagccgac
      361 actggcgctg ggcaaggtga tggttgacaa gggccacaag aaagccgtct ggattacctg
      421 gaagtacgca gccggcgagg aagccttcga gggcttccgc gacagctaca ccaaggccgg
      481 cggcacgatc gtcaaggagc tcggtgtgcc gttcccgaac gtggagttcc aggcgctgct
      541 gaccgaaatc gcctcgatca acccggatgc cgtggcctgc ttcttcgccg gcggcggcgc
      601 ggccaagttc atccgcgact atgcggctgc cggcctgaag gggcgcattc cgctgtatgg
      661 ctcaggcttc ctcaccgaag gcgtgcttga cgccgccggc cccgccgccg acggcatcct
      721 caccaccctc cactacagcg acgcgctgga caccccgagg aacaagcagt tccgcctggc
      781 ctacgccaag gcgttccgct cgcagccaga cgtctatgcg gtccagggct atgacaccgg
      841 ccagttgctg gtccaaggcg cggccgcggt tcgaggcgac ctgaacaaca agcaggcgtt
      901 gtacaaggcg ctggagtcgg ccgtcatcga cagcccacgg ggcaaatgga ccatgagcaa
      961 ggcccacaac ccggtgcagg acatctatct gcggcaggtg gtgaacaagg aaaacaaggt
     1021 gatcggcatt gccgccaagg cattggccga ttcgggcgcc ggctgcaaga tggccggcta
     1081 gcatcggcct gcccgattaa catcggccca cccgatacac tgtcaagccg ggtttgaccc
     1141 ggcatccatg tctcacgtgc ccaggtggat gccggatcaa gtccggcatg acaacctttt
     1201 gcgcccatga cgctcgccac ctacctgttt tatgtcgccg cccgcctgtg taaccggatc
     1261 tgtgggggcc ttttcaccat gaagggcggc ctgccgccgt ccacccgccg cagcgcctga
     1321 gccgatggat ctcgcgaatt tcctgatcca gctcctcaac agcgtccagt acggcctgtt
     1381 gctgttcatg ctggcggccg gcctcacgct gatcttcggc atcatgggcg tggtcaacct
     1441 cgcgcacggc agcttctaca tgctaggcgc ctacctcgcg tggtgggtct cgagcatgac
     1501 gggcagcttt accctggcga tcatcctcgg ggcgctactt tcggtggtgt tcggcctggt
     1561 gctcgagcgg ctgctgtttc gccacttcta ccaacgcgac cacctggacc aggtactgct
     1621 gaccttcggg ctgatctata tcttcgagga atcccgctcc atcctctggg gcgacgacgt
     1681 ccacggggtt aagattcccg agttgctggc ggcctcgatt ccgctgaccg acaacctgtc
     1741 gtatccggtc tatcgcctgt tcatttcggg ggtgtgcatc gcgctggcga tcggcctgta
     1801 cctgctgatt tccaagacgc gcctgggcat gaaaatccgc gcgggcgcct tcaatcgccc
     1861 catgacggag gcgctgggtg tcaacatcaa gctgatccat gcggtcgtgt tcgcgatcgg
     1921 cgtggcgctg gccacggtgg ccgggatgat cgcctcgccg atctccagcg tctatcccgg
     1981 catgggctct caggttctca tcatcagttt cgtggtggtg gtgatcggcg gcatcggctc
     2041 ggtgcgcggc gccctgatct ccgcgatgct cgtcggactg gtggacacgt tcggcaaggt
     2101 gctgctcccc gctgtggccg gcatgctgat ctacatgctg atggccgccg tgctgctgtg
     2161 gaaacccgaa ggattgttca agcaatgatg aacatggccc ccacgctcat cacttcgtgt
     2221 aattcgctgc cccccgaggg ggcgttcctc gccttcgggc ggcccagcgg cgactcatga
     2281 cagccgccgc taacctcgaa acgctgccac gcagcctgca gatcgtgctg gcgctgggct
     2341 tcctggcgct cgcggcgttc cctttcttcg gcaccgactt ctatgcgcag atggtgtcgc
     2401 ggatgatgat cctggccatc ttcgccatga gcctggacct gctgctggga gtcaccggcc
     2461 tcgtttcgct gggccatgcc gccttcttcg ggctggcagg ttacgcgctg gccttcatca
     2521 caccggccgg agaaccggtc agcctgtggt ggacgctgcc ggtggcggtc gccgcctcgg
     2581 ggcttgcagc gctggtgatc ggcttcttcg tggttcgcac ccgcggcatc tacttcatca
     2641 tggtgaccat ggcgttcgcc cagatggtgt tctacctgtt cttcgacaac aaggtcctcg
     2701 gcggctcgga cggtctctac gtgaatttca agccgaacgc ggggctgttc gaccttgaag
     2761 acaagcaggt tttctattac ttcacgctgg cggtgctgct gctggtgtac ctgttcctgc
     2821 gccggttgct ctggagcccg ttcggccgcg tgctggccgg cattcgcgtc aacgagcacc
     2881 ggatgcgcgc catgggctat cgcactttcg gctacaagct caccgccttc agcctggccg
     2941 gcgcgctggc cggactcgcc ggctatctgt ggggtgcaca gaccggctac atcaacccgg
     3001 aactcatggg ctttcacatg agcgcgcacg ccatcatgat ggtcattctg ggcggcatgg
     3061 gcaacttcgc cggtgcgatt gtgggcgcct tcgcattcga gtacctgctg cacgagttca
     3121 aggacctgcc gaccatcggc gccgtgcatc tcggaaagca ctggcagatg tggatgggcc
     3181 tgttcatcgt gctggtggtg acgttcgcgc cgcgcggcct gcttggcatg gtcgagcggc
     3241 tgtcgcgccg caaggagcgc accggtgact gagcccctgc tgtcggccaa acagctcacc
     3301 aagctctttg gcgggctggc tgccgtcaac caagtttcgg tggacctgtg gcagggccgc
     3361 atccacgcgc tcatcggccc caacggcgcg ggcaagtcca cgttgacgaa cctgctgtcg
     3421 ggtgacctgc cgccgacgac gggcacgatc acgctggacg gccgggacgt caccggctgg
     3481 acgcccgaga agatttcgcg tgtggggctg gggcgcagct accagaagac caacatcttc
     3541 ctgccgttca ccgtgtggga gaacgtgcgg ctggcggccc aatcccgcac gccccatgct
     3601 tcgcgctggc tcagccgcgc aacgggtttt gccgccatca acgaccgcgc aaaccgggcg
     3661 atcgagcttg cggggctgga agaccgcacg caccaaatcg ccggcaacat cagccacggc
     3721 gagcagcgcc agctcgagat cgcgattgcg ctcgcgaccg accccaaggt actcctgctg
     3781 gacgagccgc tggccggcat gggcatcgcc gaggcgcagc gtatggtcga tctgctgctc
     3841 aagctgaagc cggcccacgc catcatgctg gtggaacatg acatggatgc ggtgttcgca
     3901 ctggccgatc acctcaccgt gatggtcaac ggccaggtga tcgcgagcgg caagccgccg
     3961 gacgtgcgtg ccgacgcgaa cgtgcaatcg gcctacctgg gggaggagca ttgatgcgct
     4021 gtacatgcgt cctgaatccg tgcgagggtc ggccgtgagc gagctcctca tcgatgccag
     4081 ggcccttcac acctactacg gggccagcca catcctgcgc ggcatcgact tcacggttgc
     4141 gccgggcgag accatcgggc tcatgggccg caacggcatg ggcaagagca cgttgctcaa
     4201 gacgctgatc gggcacgtca agccgcgttc cggcagcgtt cgcgtcatgg ggcaggacat
     4261 gacggcccgc ccctcctatg aaatcgccca gcgcggcgtc gcttacgtgc cggagggccg
     4321 cggcatcttc ggcaacctga gtgtggagga gaacctgaag atggccgcgc ggcccggccc
     4381 gcgcggacag cgcgactgga catacgagcg cgtgctggag actttccctc gcctggggga
     4441 acgcctgcgt caaggcggcc agcagctgtc cggcggtgaa cagcagatgc tgaccatcgg
     4501 gcgcgcgctc atgaccaacc ccgacgtgct gatcctggac gaagccaccg aaggcctggc
     4561 gccgctgatc gcgcgcgaca tctggagcat ctgctcgctg atccgcgagt ccggtatctc
     4621 cagcgtcatc gtggacaaga actggaagca tgtcacgcag atcaccgatc gcaacgtgat
     4681 cctggtgaag ggcgagatcg tctttgccgg tacatcggac gagctgcgcg cgcggcccca
     4741 gctgctggaa caatacctgg gcgtatagcg agcgacgatt tcagtccggg cagctcagtc
     4801 cttcgccggt gacgtgctcg ccgaggaaat cgaggaagtg ccgcaccgcc gggatcagtc
     4861 cgcgccgcga agggaatacc gcgtgaatga cgccgggccg cggcgcccat ccgggaagca
     4921 cctgctccag ttcgccgcgc tgcagttcgc ggcggcacag gtagtccggc atgatgcaca
     4981 tgccggtgcc cttgagcacc gcgtacctga gcgtcaataa atcgtcggcc gtgtagcagg
     5041 gacgatgctg gaactcgtac tcgcgtgcgc tcgggccgct gagcagccag ctggcgcgcc
     5101 catccggggc cgacatcgcc aaagtggaca gtcgctgaag gtcctcgact gtctccggcc
     5161 ggccctgccg ctccagctgt gacggactgg ccacgaccag cccctgggag atgcccaggt
     5221 tcttcacgat catgctgcca ctgtcgtcca gcgtggagcg cacccgcagc gccacgtcca
     5281 cgccgtcctg caccaggtcg accacgcggt tgctcacctg catttcgatc cgcacatgcg
     5341 gatggcgctc caggaacagc ggaatcaacg gacccaccgt ggattgcgcc agcgtcaccg
     5401 ggcatgccac ccggatcgtg ccccgcggct cgttgtggac ggcggcgacc gcgtccgccg
     5461 ccgcctcggc ctcctcgcgc atcgcagcgc aatgccggtg gaagacctct cccgcggctg
     5521 tcagggacag cttgcgggtg gtgcgctgca acagccgcac ccccagccgc gcttcgagtt
     5581 cggcaacgcg ccgcgacagc ttggacttgg gcaggcccag cgcccgtcct gctgcggcga
     5641 aaccgccgcg atccaccacc tcggcgaagt agaacatgtc gttgagatcc tgcatgtcgg
     5701 ctttccattg tcctgcgaat aggacaatct aacccgaatt tgccgactta tcaatcattc
     5761 gatctatctc cacaatcaat accagcctga acccaaccct cactggagaa aacatcatga
     5821 gactgcttca catcgattcg agccccctgg ccggcaactc ggtttcccgc caactcaccg
     5881 agcgcatcgt tgcgcaatgg cgcgccaccc accccggcac cgtggtcgag tacctcgacc
     5941 tggcggcgga tgcgcccagc cacctgtcca tcgactcgtt gggttttcgc gccggaccgg
     6001 acgccaaggg ccagagcgag gtgcagcgcc gcgaaaacgc gatttccgag cgactggtaa
     6061 gccagtttct cgcggctgac gtgatcgtgg tgggtgcccc catgtacaac ttctccattc
     6121 cgagccagct caaggcctgg atcgaccgcg tggcgcaagc cgggcgcacc ttcacctaca
     6181 ccgaaaaagg ccctaaaggc ctggccgccg gcaagaccgt gatcgttgcc tcgacccgtg
     6241 gcggcatcta ctcctcgaac ccggcaatgg
//
LOCUS       DASWIY010000380         3816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1010207, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000380 DASWIY010000000
VERSION     DASWIY010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010207"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="VGA59_13570"
     CDS             <1..178
                     /locus_tag="VGA59_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEX9720749.1"
                     /translation="LVDEIPLAGVSPWLLGAVLEQFFARHVGLNSFSEFVLRSTQRGQ
                     IARWHPRIGRRPSV"
     gene            217..1323
                     /gene="tssG"
                     /locus_tag="VGA59_13575"
     CDS             217..1323
                     /gene="tssG"
                     /locus_tag="VGA59_13575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEX9720750.1"
                     /translation="MRLGKLFAGLEADAAQFDFYYALRHIDAAIAGAVPLGRAARPRD
                     EPIRLTQHPSLAFAPAALHQFKPGVAAHGLGRLSVHHFGLFGPNGALPQHLTEYVQER
                     ILHGKDETIARFCDIFQHRMILLFYRAWADCQAVTSVDRPDRDAFGRYAASLVGLGQA
                     SLKDRDSVPDHLKLHHAGHLARQARNAEGLVRPLAALLRVPVAVQEYCLQWLTLATDE
                     RTRLTSVALAGAEAKHRIEVGAASSRLGMGAIAGAKVPDVQHKFRLQLGSMPLHEFER
                     YLPGGLRFFQVRDWVRNYLGVELAWDARLLLKRDEVPATRLGAYGQLGWTSWLAMPPG
                     RRTRDAEDVLLDVERLSRAHPASRNPYTQEAAHV"
     gene            1316..>3816
                     /gene="tssH"
                     /locus_tag="VGA59_13580"
     CDS             1316..>3816
                     /gene="tssH"
                     /locus_tag="VGA59_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEX9720751.1"
                     /translation="MSEISRVALFGKLNPLLYKAIEGATVFCKMRGNPYVELVHWLQQ
                     ILSTNDSDLHRIVRHFEVDAAKLAADLTRALDKLPRGATAISDLSEHIENAVERAWVY
                     GTLKYGDNQVRSAYIMVGLVKTTGLRNILYGISHEFEKLGVDALADKLADIVKGSPET
                     ELSASDGTGLGGEPGEASGAMAPAQMGKQEALKKYAVDLTEKARKGEIDPVTGRDEEI
                     RQIVDILMRRRQNNPILTGEAGVGKTAVVEGFALRLARGDVPPQLKEVSLLMLDVGLL
                     QAGASMKGEFEQRLRQVIDEVQASPKPIILFVDEVHTLVGAGGAAGTGDAANLLKPAL
                     ARGTLRTIGATTWAEYKKYIEKDPALTRRFQVVQILEPEEAKAVAMLRGVATVLEKHH
                     KVLLLDEAITAAVNLSHRYIPARQLPDKAVSLLDTSCARVAVSQHATPPEVEDVQRRI
                     EMLDVEIGIIQREVDVGIETGERLAEATAKRSDAAATLASLESRWSDEKALVAQILEL
                     RGKLRASGDGRVDTAVPLDDAARSATMKELHQLQDTLAQKQGETPLILPAVDAQAVAS
                     VVADWTGIPVGRMVKNEVEAILKIADTLNQRVIGQRHGLDMIAKRIQTSRARLDNPNK
                     PIGVFMLVGPSGVGKTETALTLAETLYGGEQNMITINMSEFQEAHTVSTLKGAPPGYV
                     GYGEGGVLTEAVRRKPYSVVLLDEVEKAHSDVHEIFFQVFDKGTMEDGEGRLINFKNT
                     VIILTSNVGSELVMNMSRDPELMPEPEHIAKALREPLLKVFPAALLGRLQVIPYYPLS
                     DEMLGNIIRLQLNRIKKRIAENHKAQFDYSEEVVKL"
BASE COUNT          748 a         1174 c         1230 g          664 t
ORIGIN      
        1 gctggtcgat gagataccgc tggcgggtgt cagcccgtgg ttgctcggcg ccgtgctgga
       61 gcagtttttt gcgaggcacg tgggcttgaa ctcattttcc gaatttgtct tgcgcagcac
      121 gcagcgcggc cagattgccc gttggcaccc gcgcatcgga cggcgcccct cggtgtgaca
      181 aaacagccca acgcaaccgc ggcaacaccg ccgctgatgc ggttgggtaa gctgtttgcg
      241 ggcctggaag ccgatgcggc ccagttcgat ttctactatg ccctgcgtca catcgatgcc
      301 gctattgcag gcgccgtgcc cctggggcgc gctgcgcgac cgcgcgatga acccatacgc
      361 ctgacccagc acccatccct agcgttcgct ccggcagcgc tgcatcaatt caagcccggt
      421 gtcgcggcgc acgggctggg ccggctttcc gtgcatcatt tcgggctctt cggccccaac
      481 ggggcgctgc cccagcatct gacggagtac gttcaagagc gcattctgca tggcaaggac
      541 gaaaccatcg cgcgtttctg cgatattttc cagcaccgga tgatcctgct gttctaccgc
      601 gcctgggccg attgccaggc cgtgaccagc gtggatcggc cggatcgcga tgccttcggc
      661 cgctacgccg caagcctggt tggcctgggt caggcatcgt tgaaggaccg cgattccgtc
      721 ccggaccact tgaagctgca tcatgcgggc cacctggctc gccaggcccg caatgcggaa
      781 gggctggtac gccccttggc tgcattgctg cgcgtgcccg tggcggtcca ggagtattgc
      841 ctgcagtggc tgacactggc cactgacgaa cgcacgcgtt tgacatccgt tgccttggcg
      901 ggcgcggagg ccaaacaccg gatcgaggtg ggcgcggctt cttcgcgcct gggcatgggc
      961 gcgatagccg gcgccaaagt gcccgatgtc cagcacaagt tccgtctaca gctcggctcc
     1021 atgccgctgc acgaattcga gcgatacttg ccgggcggct tgcgcttttt ccaggtgcgc
     1081 gactgggttc gcaattacct cggtgtcgag ctggcctggg atgcccgtct gctgctcaag
     1141 cgagacgaag tgcccgcaac ccggttgggc gcgtacgggc agctcggatg gaccagttgg
     1201 ctcgctatgc ctcccggccg ccgtacgcgt gacgcggaag acgtattgct ggatgtggag
     1261 cggctgagcc gcgcgcatcc ggccagtcgc aacccctaca cacaggaagc tgcccatgtc
     1321 tgaaatcagc cgagttgcat tgtttggcaa actcaacccc ttgctctaca aagccatcga
     1381 gggggccacc gtattctgca agatgcgtgg caacccctat gtggagttgg tccactggct
     1441 ccagcagatc ctgagcacga acgacagcga tttgcatcgc atcgtgcgtc acttcgaggt
     1501 tgacgcggcc aagctggccg ccgacctgac gcgtgcactg gacaagctgc cccgtggcgc
     1561 aacggcgatt tccgatctct cggaacatat cgagaacgcg gtcgaacggg cgtgggtcta
     1621 cggtacgctg aagtatggcg acaaccaggt acgcagtgcc tacatcatgg tgggcctggt
     1681 caagaccacc gggttacgca atatcctgta tggcatctcg cacgagttcg aaaaactcgg
     1741 cgtcgatgcg ctggccgaca agctggccga cattgtcaag ggatcgcctg aaaccgaatt
     1801 gtccgccagc gacggcacgg gcttgggcgg cgaaccgggc gaggccagcg gcgccatggc
     1861 accggcgcag atgggcaagc aggaagcctt gaaaaagtac gcggtcgacc tgacggaaaa
     1921 agcccgtaaa ggcgagatcg accccgtgac ggggcgcgac gaggagattc gccagatcgt
     1981 ggacatcctg atgcggcgcc ggcaaaacaa ccccatcctg accggcgagg cgggagtcgg
     2041 aaagaccgct gtggtcgaag gctttgcctt gcgtctggcc cggggggatg tgccgcccca
     2101 gttgaaggaa gtctccttgc tcatgctgga tgtgggcctg ctgcaggcag gtgccagcat
     2161 gaagggcgag ttcgagcagc ggctgcgcca ggtgatcgac gaggttcaag ccagccccaa
     2221 acccatcatc ttgttcgtgg acgaagtgca cacccttgta ggcgcgggcg gcgccgccgg
     2281 aacgggtgat gccgccaacc tgctcaagcc cgcgctcgcg cgtggaaccc tgcgcaccat
     2341 cggcgccacc acctgggccg aatacaagaa atacatcgaa aaagacccgg cactgacccg
     2401 ccgcttccag gtcgtccaaa ttctcgaacc cgaagaggcc aaggccgtcg cgatgctgcg
     2461 cggcgtggcg accgtgctgg aaaagcatca caaggtgctg ctcctggacg aagccattac
     2521 cgcggcggtc aatctttcgc accgctatat acccgcccgc caattgcctg acaaggcggt
     2581 gagcctgctc gataccagct gcgcccgcgt ggcggtgagc cagcacgcca cgccgccgga
     2641 ggtggaagac gttcagcgtc gcatcgaaat gctggatgtg gagatcggca tcatccagcg
     2701 ggaagtcgat gtcggcatcg aaacgggtga acggcttgcg gaggctacgg ccaagcgatc
     2761 ggacgcggca gccaccctgg catcgctcga gtcccgctgg tcggacgaaa aggccttggt
     2821 cgcgcaaatc ctggagctgc gcggcaagtt gcgcgccagt ggtgatgggc gggtcgatac
     2881 cgccgtcccc ctggacgatg cagcccgcag cgccacgatg aaagagctcc accagttgca
     2941 ggatacgctg gcgcagaagc agggcgagac gccgctgatc ctgccggcgg tggacgcaca
     3001 agccgtggcg tcggtcgtgg cggactggac gggtattccg gtcggccgga tggtcaagaa
     3061 cgaggtcgaa gccatcctga agattgccga cacactcaac cagcgggtga taggtcagcg
     3121 gcatggcctg gacatgatcg ccaaacggat tcaaaccagc cgcgcccggc tggacaaccc
     3181 gaacaagccg attggcgtgt tcatgctggt tggcccgtca ggtgtgggca agaccgaaac
     3241 ggcgctgacc ctggccgaga cgctctatgg cggtgagcaa aatatgatca ccatcaatat
     3301 gagcgagttc caggaggccc ataccgtctc gacgctcaag ggcgcccccc cggggtatgt
     3361 gggctatggc gaaggcggcg tcttgaccga ggcggtgcga cgcaagcctt acagcgtggt
     3421 gctgctggac gaggtcgaaa aggctcacag cgacgtgcac gagatattct tccaggtgtt
     3481 cgacaaaggc accatggaag acggcgaagg ccgtttgatc aatttcaaga acacggtgat
     3541 cattctcacc agcaacgtcg gaagcgaact ggtgatgaac atgagccgcg accccgaact
     3601 gatgccggag ccggagcaca tcgccaaagc cctgcgcgag cccttgttga aggtgtttcc
     3661 cgcggcgctg ctgggccgtc tgcaggtcat tccctactac ccgctcagcg acgagatgct
     3721 gggcaacatc attcgcctgc agctgaatcg catcaagaaa cggattgcgg aaaaccacaa
     3781 ggcgcagttc gactattcgg aggaggtcgt caagct
//
LOCUS       DASWIY010000381         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3565558, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000381 DASWIY010000000
VERSION     DASWIY010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3565558"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VGA59_13585"
     CDS             complement(<1..910)
                     /locus_tag="VGA59_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX9720752.1"
                     /translation="MSSTHLATALVVPFEQLRMTDVESVGGKNASLGEMISQLAATGV
                     RVPGGFATTAHAFRQFLKHNGLAERINARLAALNTDDVRALAEAGAQIRQWVEETPFP
                     ADLQAAIAAEFDKLSAGNPGASFAVRSSATAEDLPDASFAGQQETFLNVVGISEVLHK
                     MKEVFASLYNDRAISYRVHKGYAHTDVALSAGVQRMVRSDLGAAGVVFTIDTESGFKD
                     VVFITSSYGLGETVVQGAVNPDEFYVHKPALQRGKLPIIRRNLGSKLIKMEFATAAEK
                     AASGKLVKTVDTAPELRNRYSLSDADV"
     gene            1142..1963
                     /locus_tag="VGA59_13590"
     CDS             1142..1963
                     /locus_tag="VGA59_13590"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX9720753.1"
                     /translation="MPNRTVFFVSDGTGITAETFGNSILNQFAIKPRHVRRPFIDTVD
                     KAHQVVREINHTAETEGKRPVAFITLVDRDVLAIVRDHSKALVLDMFNTFIEPLEAEF
                     AVTSNHRVGRFSDVSKSQEYTDRIEAINFSLAHDDGQSAKNLESADVILVGVSRSGKT
                     PTSLYLAMQHGIKAANYPLIPEDFERHQLPSMLAPHKGKCFGLTIDPARLNQIRNERR
                     PGSKYAALENCRFEVREAEAMMRREGIAWLSSTHKSIEEIATTILRDLRPDRLMY"
     gene            complement(1968..>2596)
                     /locus_tag="VGA59_13595"
     CDS             complement(1968..>2596)
                     /locus_tag="VGA59_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9720754.1"
                     /translation="GVPVHAVITEPAWEQAALRTLASHAAEVAVVPPALMGSISTLES
                     PPAVVFAVPWVGVGSVMRAVPSVVLDRLQDAGNVGAILRSASAFGFTQIVAIKGTAAL
                     WSPKVLRAGMGAHFGLNLVEGVEAVPDLGVPLLASSSHAERAIHAVRLPWPCGWILGH
                     EGQGISEALMQHCELTLRIPQPGGEESLNVAAAAAVCLYESARQRDTA"
BASE COUNT          447 a          897 c          826 g          426 t
ORIGIN      
        1 tcacgtcggc gtcgctgagc gaatagcggt tgcgcagctc gggcgccgtg tccaccgtct
       61 tgaccagctt gcccgatgct gccttttccg cggccgtggc gaactccatc ttgatcagct
      121 tggagccgag gttgcggcgg atgatgggca gcttgccgcg ctgcagcgcc ggcttgtgca
      181 cgtagaactc gtcggggttg acggcgccct gcaccactgt ttcgccgagg ccgtagctgg
      241 aggtgatgaa caccacgtcc ttgaagccgc tctcggtgtc gatggtgaac accacgccgg
      301 ccgcaccgag gtcggagcgc accatgcgct gcacgccggc cgacaaggcg acgtcggtgt
      361 gcgcatagcc cttgtgcacg cggtagctga tcgcacggtc gttgtagagg ctggcaaaca
      421 cctccttcat cttgtgcagc acctcggaga tgcccaccac gttgaggaag gtctcttgct
      481 ggccggcaaa gctcgcatcg ggcaggtctt cggccgtggc cgacgagcgc acggcaaacg
      541 acgcaccagg gttgccggcg ctcagcttgt cgaactccgc ggcgatggct gcctgcaggt
      601 cggccggaaa cggcgtctcc tcgacccatt ggcggatttg cgcgccggct tcggccagcg
      661 cacgcacgtc gtcggtgttc aacgcggcca ggcgcgcgtt gatgcgttcg gccaggccgt
      721 tgtgcttgag gaattggcgg aacgcgtgcg ccgtggtggc aaagccgccg ggcacgcgca
      781 cacccgtcgc ggccagttgg ctgatcattt cgccgagcga ggcgttcttg ccgcccaccg
      841 actcgacgtc ggtcattctc agttgttcaa acggtacgac cagggcggtc gccagatggg
      901 tggatgacat gggaaagctc cgtgatgttg gtaaaccggt gcttcccgac cctggcgtgg
      961 cgatcccgag ccgtgctcaa gggatgcaat ggcgaaggcg aaggcgcacg cccgaccctg
     1021 ttcgagttct tggcggtggg aggtggcgct catcggtggc gggcaggaag gattgcctgg
     1081 gattctacgg gtgaactccc tatgatcacg ccacgcccac cgacgcccgc cctcaacccc
     1141 catgccaaat cgcaccgttt tctttgtttc cgacggcacc ggcatcaccg ccgaaacctt
     1201 cggcaactcg atcctgaacc agtttgccat caagccgcgc cacgtgcgcc gccccttcat
     1261 cgacacggtg gacaaggcgc accaggtggt gcgtgaaatc aaccacacgg ccgagaccga
     1321 aggcaagcgg cccgtcgcct tcatcaccct ggtggaccgc gacgtgctgg ccatcgtgcg
     1381 cgaccactcc aaggccctgg tgctggacat gttcaacacc ttcatcgaac cgctggaggc
     1441 cgagttcgcc gtcacctcca accaccgcgt cggccgcttc tccgacgtgt ccaagagcca
     1501 ggagtacacc gaccgcatcg aggccatcaa cttctcgctg gcccacgacg acggccagtc
     1561 ggccaagaac ctggagtccg ccgacgtgat cctggtcggc gtgagccgca gcggcaagac
     1621 gccgacctcg ctgtaccttg cgatgcagca cggcatcaag gccgccaact acccgctgat
     1681 cccggaagac ttcgagcgcc accaactgcc ctcgatgctg gcgccgcaca agggcaagtg
     1741 ttttgggctc accatcgacc cggctcggct gaaccagatc cgcaacgagc gccgcccggg
     1801 gagcaagtac gccgcgctgg agaattgccg cttcgaagtg cgcgaggccg aggcgatgat
     1861 gcgccgcgaa ggcattgcgt ggctgtcgtc gacgcacaag tcgatcgagg aaatcgcgac
     1921 gaccatcctg cgcgatctgc ggccggatcg cttgatgtac tgagtgacta cgccgtgtcg
     1981 cgctggcgtg ccgattcgta caggcacacg gcggcagccg ccgcgacgtt gagcgattcc
     2041 tcgccgcccg gctgcgggat gcgcagcgtg agttcgcagt gctgcatcag cgcttccgag
     2101 atgccctgcc cttcgtgccc caggatccag ccacaaggcc aaggcaggcg cacggcgtga
     2161 atcgcccgct cggcatgcga actgctcgcc aacagcggca ccccaagatc gggcacagcc
     2221 tccacgcctt cgaccaggtt cagcccgaaa tgcgccccca tgccggcacg cagtaccttg
     2281 ggcgaccaca gcgccgccgt gcccttgatc gcgacgatct gcgtgaagcc gaaggccgag
     2341 gcgctgcgca ggatggcacc cacgttgccg gcgtcctgca gccgatcgag caccacgctt
     2401 ggcactgcgc gcatgacgct gcccaccccg acccacggca ccgcaaacac caccgccggc
     2461 ggcgactcca gcgtgctgat gctgcccatc aacgccggcg gcaccaccgc cacctcggcg
     2521 gcatggctgg cgagcgtgcg cagcgccgct tgctcccatg ccggctcggt gatgacggcg
     2581 tgcacgggca cgccgc
//
LOCUS       DASWIY010000382        22330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2493654, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000382 DASWIY010000000
VERSION     DASWIY010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 22330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22330
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493654"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1143)
                     /gene="dnaJ"
                     /locus_tag="VGA59_13600"
     CDS             complement(1..1143)
                     /gene="dnaJ"
                     /locus_tag="VGA59_13600"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX9720755.1"
                     /translation="MATKRDYYETLGVPKNASEEEIKKAYRKLAMKHHPDRHQGEDKG
                     AEAKFKEAKEAYEMLSSPEKRAAYDQYGHAGVDPNMRGHGAGAEGFGGFAEAFGDIFG
                     DIFGQQRGAGGRGGRQIYRGSDLSYAMEITLEEAALGKDAQIRIPSWEQCDSCHGSGA
                     KPGTQAKTCSTCHGQGVVQMRQGFFSVQQTCPHCRGSGKIIPEPCTTCHGQGRVKKQK
                     TLEVKIPAGIDDGMRIRSAGNGEPGTNGGPPGDLYIEIRLRKHDIFERDGDDLHCVVP
                     ISFTRAALGGEIEVPTLQGKAAIDIPEGTQAGKQFRLRGKGIKGVRSSYPGDLYCHVA
                     VETPVKLTEHQRKLLNELDESLKKGGAKHSPTEESWADKLRGFFSA"
     gene            complement(1257..3206)
                     /gene="dnaK"
                     /locus_tag="VGA59_13605"
     CDS             complement(1257..3206)
                     /gene="dnaK"
                     /locus_tag="VGA59_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX9720756.1"
                     /translation="MGRIIGIDLGTTNSCVAIMEGNTPKVIENSEGARTTPSIVAYQE
                     DGEILVGASAKRQAVTNAKNTLYAVKRLIGRKFEEKEVQKDIHLMPYKIVKADNGDAW
                     VEVRGKKLASPQISAEVLRKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGLDKQERGDRKIAVYDLGGGTFDISIIEIADVDGEKQFE
                     VLSTNGDTFLGGEDFDQRIIDYIIAEFKKDQGVDLSKDVLALQRLKEAAEKAKIELSS
                     NSQTDVNLPYITADANGPRHLNIKLTRAKLEALVEELIARTIAPCRTAIKDAGVSISD
                     IHDVILVGGQTRMPKVQEKVKELFGKEPRKDVNPDEAVAVGAAIQGQVLGGERKDVLL
                     LDVTPLSLGIETLGGVMTKMITKNTTIPTKFSQTFSTADDNQPAVTIKVYQGERDIAT
                     GNKLLGEFNLEGIPPAPRGLPQIEVSFDIDANGILHVGAKDKATGKENKITIKANSGI
                     SEEEIQKMVKDAELNAAEDKKKLEIVQARNQGEALVHSVRKSLAEYGDKLDAGEKEKI
                     ESALKDVEEALKGEDKSAIEEKTNTLMTASQKLGEKMYSDMQAKQAAEAAGPGGGGGG
                     GAESASSNPSDDNVVDAEVKEVKKG"
     gene            complement(3327..3908)
                     /gene="grpE"
                     /locus_tag="VGA59_13610"
     CDS             complement(3327..3908)
                     /gene="grpE"
                     /locus_tag="VGA59_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX9720757.1"
                     /translation="MSDNNPSEPARPEAPTALDMSPEEREAAKAANENDLLGQLQADL
                     AGMQAKNAELAEQFLRAQADVQNTRRRAEDEISKARKFAVESFAESLLPVVDSLEASF
                     AHEDATREQLREGSEATLRQLKSALERNKVVEINPAVGAKFNPHQQQAISVVPLPPDS
                     TQEANTVVSVLQKGYLIADRVLRPALVTVAAPK"
     gene            complement(4014..4349)
                     /locus_tag="VGA59_13615"
     CDS             complement(4014..4349)
                     /locus_tag="VGA59_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9720758.1"
                     /translation="MTPSSPLSGKTVRGSRTGRPVMALLDLLGRRGSLRLLWELRDGH
                     PQSFRLLRASADQISPSVLNTRLKELREARLVELAQAGYGLTPSGLELVRHLRPLNRW
                     AEAWADALS"
     gene            4470..5057
                     /locus_tag="VGA59_13620"
     CDS             4470..5057
                     /locus_tag="VGA59_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9720759.1"
                     /translation="MSVTPLPPRIEPLSPPYTPELEALLQRMTPPGAPSILALFRVLA
                     HHPVLAERMSGFGSLLLGRKASLSLRDREVVIDRVCARCGAEYEWGVHVAAFGEAAGF
                     TPAQNTAIADIDSNAAPLTPRDRLLVRMVDELHESGTVSDALWPELASLWSTQQLLEL
                     LVLAGWYHAIGYVCNAARVPLEPWAAKWDGLTPRA"
     gene            complement(5069..5419)
                     /locus_tag="VGA59_13625"
     CDS             complement(5069..5419)
                     /locus_tag="VGA59_13625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX9720760.1"
                     /translation="MITTPSGLQYDDTTVGAGAEAKPGQHVHVHYTGWLFNDGRQGSK
                     FDSSVDRNEPFAFTLGSGMVIKGWDEGVAGMKVGGKRTLVIPPQLGYGARGAGGAIPP
                     NATLKFDVELLDAH"
     gene            complement(5499..6674)
                     /locus_tag="VGA59_13630"
     CDS             complement(5499..6674)
                     /locus_tag="VGA59_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9720761.1"
                     /translation="MKQYLQIQTTLPADADAALLVGRVHIAGTGPMLVQVRPDGVYDL
                     SGLAATCSELLELPDLADSVRDNRGARIAATQEVLANSAFDARHAAKPWFLAPCDLQA
                     IKAAGVTFVASMLERVIEEQARGDASCAEAVRQAVVAVIGDDLAAVQPGSASAAQLKD
                     VLVAQGMWSQYLEVGIGPDAEIFTKSQPMSSVGTGAEVGIHPSSSWNNPEPEIVLAVN
                     SRGEVRGAALGNDVNLRDFEGRSALLLGKAKDNNASCAIGPFIRLFDEHFGMADVGRC
                     DLAMQVEGRGGFRMQGSSSLAKISRDPLDLVSQAIGANHQYPDGFVLFLGTMFAPTQD
                     RHGPGQGFTHEIGDLVTVSTPRLGTLANRVNTSDKVAPWTYGAAALMRDLARRGLLG"
     gene            complement(6671..6982)
                     /locus_tag="VGA59_13635"
     CDS             complement(6671..6982)
                     /locus_tag="VGA59_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX9720762.1"
                     /translation="MGSKENAAISQLLGLLESRYAIRVLWALRDGHAQTFRLLQDSVG
                     GITPNTLNTRIKELREAGLVNHGSDGYVVTPAGLDLLKRLGELPAFATKWVASQAKKK
                     S"
     gene            complement(7091..7612)
                     /locus_tag="VGA59_13640"
     CDS             complement(7091..7612)
                     /locus_tag="VGA59_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9720763.1"
                     /translation="MIARTLKTLQVRNTPAVEEVDTGYLDGLIGYNARRAALAVIDVF
                     LERMKVYDLRPVDFSVLSLIMHNPGITSRQLCATLGILPPNLVGMINALEKRKIIMRK
                     PHPRDGRALGLHVTGPGQKLMRDAERTAAELETEVASRLTAAESKTLIRLLQKVYLER
                     DQPAARALQPQKK"
     gene            complement(7609..9369)
                     /locus_tag="VGA59_13645"
     CDS             complement(7609..9369)
                     /locus_tag="VGA59_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9720764.1"
                     /translation="MNWNPAQDDIRFMLFDVLRADAQLAAMPAFAEADRELMSQVIEQ
                     AGKFVAEVVAPLQGAGDSPGCRWQDGDVTTPPGFREAYQAFWQGGWPALACAPADGGQ
                     GLPGVLEAALYEMLSGANHGWTMAPALLHGAYECLRHHGSEGLKARYLGKIASGEWLA
                     TMCLTEAHAGSDLGLVRTRAMAQADGSWRLNGSKIFISGGEHDLTDNIVHLVLARTPD
                     APAGPKGLSLYLVPKVLPAGTRNGVHCDRIEEKMGLHGSPTCAMRFEDATGWLVGETS
                     SGLNAMFVMMNAARLHVALQGLGLLDAAWQKAEAYALERRQMRVPGRRDGKEAADAIL
                     GHPAIRRILDTQRAWVDGGRVLAYQTALELDIAKHHPQAERRASAQRWCALVTPVLKA
                     ALTEQAFHGASACLQVFGGHGYVREWGIEQIVRDSRVAMIYEGTNEIQAIDLVVRKVL
                     ADGGAGLAALLSGLDADLDASRPAHADVLCRFAELRDLTARLAQAALHDPVLPFEAAD
                     DYLRALALALLAWAWTRIETAAPAGVPRWSRSAAALRLRVLPEFDMRIKIIQGQCAAA
                     SSRPEAPAVAAYVQKSVSAP"
     gene            complement(9378..10115)
                     /locus_tag="VGA59_13650"
     CDS             complement(9378..10115)
                     /locus_tag="VGA59_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720765.1"
                     /translation="MTTPMLRIDNLHAGYGRAEVLHGLALEAAPGSVVTVIGPNGAGK
                     STLLNTLMGVIPSRGTLQFDGQDIGVMPLEERVMMGMALVPERRELFGSMTVEDNLVL
                     GGYRQVQLGNREWRARIDDVYAIFPRLKERRLQLAGTLSGGERQMLAVGRALMSRPRL
                     LMLDEPSLGLAPLIVLDIFRTIDSLRQTGVTILLVEQNARAALEVADHGYVLEMGEIA
                     LEGSAQELASDPRVIDTYLGAAIHADD"
     gene            complement(10125..11942)
                     /locus_tag="VGA59_13655"
     CDS             complement(10125..11942)
                     /locus_tag="VGA59_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX9720766.1"
                     /translation="MNSEPVSIARMATTGPLLSRRQLGLIALAAAVPLVALLPLPDFW
                     ITQLNYIGLYSLTCLGLVLLTGIGGLTSFGQAAFVGIGAYTTAWLTVNTGISPWLTLP
                     IGLAITGASALLLALITLRMSGHYLPLATIAWGLSLYYLMGNIDALGKYDGILGIKSL
                     TFFGLDIGQGRGFFVLTWAIVLALATALLHLLDSRAGRAIRALRSGSQMAESMGISTF
                     RYKVIIFLIAALFASVAGWLFAHFQRTVTPTSFGLKMGIEYLFMTVVGGVGHVWGALT
                     GAALTKLLDDQLQVLLPRLIGTSGSYEVIVFGVALVLMLKYQPDGLWDFVARRLPRRD
                     RTVDWADAPALPEKPKPLSGELLLDVRAVRKEFGGLVAVNDVSFQLRAGEIVGLIGPN
                     GAGKSTIFNLITGVLSLTGGAVTFRGESLGGQRARAIARRGLGRTFQHVKMIPDMTVL
                     ENVALGAHARGRKDVLAAMARLDRAEERALFKEAQRQLERIGMGAWLHELAGNLPMGQ
                     QRLLEIARALCSDPALLLLDEPAAGLRHKEKRALIDVLRQLQREGMSILLVEHDMDLV
                     MDVCDRLVVMEFGTLLMEGTPREVQRSPEVRAAYLGTEH"
     gene            complement(11939..12979)
                     /locus_tag="VGA59_13660"
     CDS             complement(11939..12979)
                     /locus_tag="VGA59_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9720767.1"
                     /translation="MDLSIASVLLIDGATNGAIYALLGLATVLVFTVTRVIFIPQGEF
                     VAYGALTLAILQTGKVPGSVWLLLVLAAVAALMQVWGEWQRHRHLLVALKSGLRTLAF
                     PVAAAAVTAFAAPHQPPLAIQALLSVLVVTAYGPLVYRVAFQSLAHASPLVLLIVSVG
                     VHFAMTGLGLLFFGAEGYRNPSFWDLRISLGIVNLTGQAIIIALASIALIVLLWLFFE
                     RTLRGKALRATAVNRLGARLMGISPQSAGQMTFAMAALIGALSGLLIGPTTTVFYDSG
                     FIIGLKGFVAAVMAGLSSYPGALLGALFVGVVESFGSFWASAFKEVIVFTLILPVLLV
                     RSLYGVHADDEA"
     gene            complement(13145..14275)
                     /locus_tag="VGA59_13665"
     CDS             complement(13145..14275)
                     /locus_tag="VGA59_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9720768.1"
                     /translation="MKFHKTLIAIATLVASAAALADVNIGVSLSLTGPGSGLGIPMQN
                     YYKLFPQTIAGEKVNLIILDDASDPGKGVSNARRFVTDDKVDMIIGSTLTTVAAAMAE
                     VAAESGTVQLAASPAGVPPGKDAWMFRLPQSNTVMSHPMVAHMAKQGVKTVGFLGYTD
                     AYGEQWLKEFTPIAEKAGIKVIAVERFARPDTSVTPQALKLVSANPDAMLIVATGSGA
                     AMPHKAIVERGFKGKIYQTHAAATKDLMRVGGKDVEGAYVVSGPAVIAEQLPETHPSK
                     KLAIDFVQKYEKLVGPGTRNQFAAHAYDSQIVLEKILPVALKKAKPGTKEFRAALREA
                     MENMGRTTFSHGVMNWSKNDHWGFTNETGVMLKVVNGEFKVE"
     gene            complement(14341..16212)
                     /locus_tag="VGA59_13670"
     CDS             complement(14341..16212)
                     /locus_tag="VGA59_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HEX9720769.1"
                     /translation="MSSPKYRALEFPVSRVILRESAGGVCYMKAEQPLREHAPRLTDR
                     LVHWARTAPDRIFMARRVRKADGTTGDWRRISYSEALDSARRIGQALIDRGLNAERPV
                     AILSENDLEHALLALGCLYAGVPFCPVSPAYSTISQDHEKLRHALATLTPGLVFAADA
                     ARYGKAIAATVGPEVELVLGNGALEERGTTPFAALLATTATTAVDAAMQATGPQTIAK
                     FLFTSGSTKLPKAVINTHGMWCANQQQMRQSMPVLAEEPPVLVDWLPWNHTFGGNHDF
                     GLTVYNGGTLYIDDGKPVPALIGETLRNLREIAPTVYFNVPTGFEAIANAMKTDDVLR
                     RNLLSRVRMFFYAGAALAQPVWDTLHEVQEREIGERIVMGTGLGMTESAPFAVFITNP
                     NVKAGYLGVPTPGLELKLVPTDGKIEVRYKGPNITPGYWRNEAATRETFDEEGFFRTG
                     DAVLWIDPDDVHQGLKFDGRIAEDFKLATGTFVSVGPLRARIIAAGAPYIQDAVITGI
                     NLHEVGALVIPTAAVRKLAGLPADAPLKDVLESAPVQAHFQRVADELAGSATGSATRV
                     ARMHLMHEPPSIDKGEATDKGSINQRAVLQHREALVNALHAGTLPFTIQPRGLVRTG"
     gene            complement(16398..17594)
                     /locus_tag="VGA59_13675"
     CDS             complement(16398..17594)
                     /locus_tag="VGA59_13675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9720770.1"
                     /translation="MAHAYIPYGLYWSTPFAKWQGPIAHLNSLRLAANTARQALEAKR
                     FPMERIDLGILGHTNPQHGSFYGLPYVTGMMGLENVAGPTVQQACATSARGLQMAAAE
                     VADGSSRCALLVTTDRCSNGPIVYYPDPTAPGGTGHTERWVLDNFAHDPHAKNAMIDT
                     AENVAAKFSIGTSQQNEVALARYAQYRQALADDRAFQRRYMVDVPITDAGFRKQIGTL
                     SADDGIFPTTVEGLAKLKPVKPGGTVTFGSQTHPADGNAGAIVTTREIAREVSLDPKI
                     EVELLGFGQARVEKGHMPMAVAPAAMAALKNAGLGIKDVAAVKTHNPFAVNDIAFALE
                     TGFPLEKMNNYGCSLIWGHPQGPTGVRSVIELIEELALRGGGVGLFGGCAAGDSGMAL
                     VLRVGG"
     gene            complement(17610..19721)
                     /locus_tag="VGA59_13680"
     CDS             complement(17610..19721)
                     /locus_tag="VGA59_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9720771.1"
                     /translation="MSEAVSYSMTGDVAVVMIDSPPVNAVNQSVRAGLKRVFAELRGL
                     PGTRAIVLGCAGKTFVAGADIKEFDTGIAPPGYHEVLRMIEDSPVPVVAAVHGTALGA
                     GTEIALACHYRVAHEGARFGLPELSLGIIPGAGGTQRLPRIVPLDVALDMALTGKPLP
                     AAKAAEAGLADLVVGNEVVAAAVAYARKLVAGGAGPRRTREQPVKGAEQAAELFAAKR
                     AQVARTMRNRQSPLALITALQAAVEKPFDQGLQVESELSSQLEQATESKALRHLFYAE
                     REVRKIPGLAPDITPRPIARVGIVGAGTMGGGIAMCCVNVGIPVTLVDAAQQNLDRGM
                     ATIRKNYERSVSRGSLKPEQLEERIRLITPSLDFAALADADLIIEAVFENLALKKEIF
                     GKLDAVAKPGAVLGTNTSTLDIDEIAGVTRRPQDVIGLHFFSPANVMQLLEIVQAGKT
                     APDVVVTALELARRIRKTGVVAKVCYGFIGNRMMDPYGREAERCVLEGATPAEVDGAL
                     EQWGMAMGILAVYDMAGVDVGYLTRLERKHLLPQDPSFYRPTAVLYEHGWLGQKAGRG
                     YYRYDGAERKRTPDPEVIALLREEAKRLDIPQRNPGAQEIQERCLYAMINEGALLLEE
                     GIALRASDIDVVYTAGYGFPRYRGGPMFYADTVGLKVVYDKIMEFQKTLDPQYWQPAP
                     LLKKLALSGSSFGQWQTEQSR"
     gene            19919..20782
                     /locus_tag="VGA59_13685"
     CDS             19919..20782
                     /locus_tag="VGA59_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9720772.1"
                     /translation="MDHQNLIAIDVHTHAEVSCWNPFDNYGEEYDRAADKYFGSNRRP
                     TIAETIAYYRERRIGLVMFTVDSESHLGRRRIPNEEIAQAAKENSDMMMCFASIDPHK
                     GKMGAREAERLISEHGVKGFKFHPTVQGYHPYDKMAWPIYEVVNAHKLPAIFHTGHSG
                     IGAGMRCGGGLRLEYSNPMHLDDVAISFPDMQIVMAHPSFPWQDEALSVATHKPNVWI
                     DLSGWSPKYFPKQLVQYANTLLKDRVLFGSDYPLITPERWMKDFEEAGFKPEVMPGIL
                     KGNAVRLLGLG"
     gene            complement(20804..21937)
                     /locus_tag="VGA59_13690"
     CDS             complement(20804..21937)
                     /locus_tag="VGA59_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9720773.1"
                     /translation="MQKLVFPALLLGALAGGAHAGDAGVMPREIRIGASAVLSGPLGP
                     QTREYGVGSRLYLDSINDKGGVYGRKIVYTTLDDAFDVGKALENTRKLIEEDKVFLIY
                     NNSGTGHTAAILPMMEDSRTIVFGPITGASIFRDRFNRYLFHVRASYANEARRIVSQL
                     KQTGVTRVAAFYQDDTFGKTLLGEIRKAAEKEKLQLVAEAKVDPKQPDFNAAAAAAEK
                     AEPQVIIMCTAGTTFPNFVKAMRQTGVKPSFYGFSVASLDVINKELGSGAQGIVLSQI
                     MPSLKNTSIPVVLEYQKLLAEKTPGAKPSASQFEGFVHAKLLVEGLRRGGKNLTTASF
                     IKGMEAAGEVRYGRFTASYSPQSHNGSSYVELAIIDSSGQLRY"
     gene            22124..>22330
                     /locus_tag="VGA59_13695"
     CDS             22124..>22330
                     /locus_tag="VGA59_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720774.1"
                     /translation="MAFPIERKAVKAQANPNKQLLQDVFAGLAEARITACTGRRGRRR
                     PWRLLKSEEGKLARQQQARQPQRNR"
BASE COUNT         3521 a         7323 c         7383 g         4103 t
ORIGIN      
        1 tcaggcgctg aagaagccgc ggagcttgtc cgcccagctc tcctcggtgg gcgagtgctt
       61 ggccccgcct tttttcagcg attcgtccag ttcgttgagc agcttgcgct ggtgttcggt
      121 gagcttgacc ggcgtctcca cggcgacgtg gcagtacagg tcgcccggat agctcgaacg
      181 cacgcccttg atccccttgc cgcgcaggcg gaactgcttg ccggcctgcg taccttcggg
      241 aatgtcgatc gcggccttgc cttgcagtgt cggaacctcg atctcgccgc ccagggccgc
      301 gcgcgtgaaa ctgatcggca ccacgcagtg caggtcgtcg ccgtcacgct cgaagatatc
      361 gtgttttcgc aggcggatct cgatataaag gtcgcccggc ggcccgccgt tggtgcccgg
      421 ctcgccgttg ccggcgctgc ggatgcgcat gccgtcgtcg atgcccgcgg ggatcttgac
      481 ttccagcgtc ttctgcttct tgacccgtcc ctgcccatgg caggtggtgc agggttcggg
      541 aatgatcttg ccggagcccc ggcagtgcgg gcaggtctgc tggacgctga agaaaccctg
      601 ccgcatctgg accacgccct gcccatggca ggtgctgcag gtcttggcct gggtgccggg
      661 cttggcgccg ctgccgtggc agctgtcgca ttgttcccag gaggggatgc ggatctgcgc
      721 gtccttgccc aacgccgctt cctccagcgt gatctccatc gcgtaggaaa ggtcgctgcc
      781 gcggtagatc tggcgcccgc cgcgcccgcc ggcgccgcgc tgctgcccga agatgtcgcc
      841 gaagatatcc ccgaatgcct cggcgaagcc gccgaagccc tcggcgccgg ccccgtgccc
      901 gcgcatgttg gggtccacgc ccgcatggcc gtattggtcg taggccgcgc gcttttcggg
      961 gctcgacagc atttcgtagg cttccttggc ctccttgaac ttggcttccg cccccttgtc
     1021 ctcaccctgg tgacggtcag ggtgatgctt catcgccagc ttgcgatagg ccttcttgat
     1081 ttcttcctcc gaggcgttct tgggaacgcc cagggtttcg taatagtctc tcttggtggc
     1141 cattgtttgt tcgtcaacca taaagcaaat cgcgcgccag ctaaaacgcc gcgttgacga
     1201 ttcccgctag ggcatccgtc gaacgcggcg acttgattcg acgctacgcg tccgaatcaa
     1261 cctttcttta cttccttcac ttcggcgtcg accacattgt cgtcggacgg gttcgaagac
     1321 gcagattctg cgccaccgcc accgccacca ccggggcccg ccgcctccgc ggcctgtttg
     1381 gcctgcatgt cggagtacat cttttcgccc agcttctggc tggccgtcat cagcgtattg
     1441 gtcttttcct cgatggcgga cttgtcctcg cccttgagcg cttcctcgac gtccttgagc
     1501 gcggattcga ttttttcctt ctcgccggcg tcgagcttgt cgccgtactc ggccaggctc
     1561 ttcctgacgc tgtgcaccag cgcttcaccc tggttgcggg cctgcacgat ctcgagcttt
     1621 ttcttgtcct ccgcggcgtt gagctcggcg tccttcacca tcttctggat ctcttcctcc
     1681 gaaatgccgg agttcgcctt gatggtgatc ttgttctcct tgcccgtggc cttgtccttg
     1741 gcgcccacgt gcaggatgcc gttggcgtcg atgtcgaagc tcacttcgat ctggggcaat
     1801 ccgcgcggcg ccggcggaat gccttcgagg ttgaactcgc ccagcagctt gttgccggtg
     1861 gcgatgtcgc gctcgccctg gtagaccttg atcgtcacgg ccggctggtt gtcgtcggcc
     1921 gtagagaagg tctgcgagaa cttggtcggg atggtcgtgt tcttggtgat catcttggtc
     1981 atcacgccac cgagcgtctc gatgcccagc gacagcggcg tcacgtccag cagcagcacg
     2041 tccttgcgct cgcctcccag cacctggccc tggatcgccg cgcccacggc aacggcttca
     2101 tcggggttca cgtccttgcg cggctccttg ccgaacagct ccttcacttt ttcctgcacc
     2161 ttgggcatgc gggtctggcc gcccaccagg atcacgtcgt ggatgtcaga gatcgacacg
     2221 cccgcatcct tgatggcggt gcggcacggc gcgatggtgc gcgctatcag ttcctccacc
     2281 agcgcttcca gcttggcgcg ggtgagcttg atgttcaggt ggcgcggacc gttcgcgtcg
     2341 gccgtgatgt agggcaggtt cacgtcggtc tgcgaattgc tggacagctc gatcttggcc
     2401 ttctccgcgg cttccttcag gcgctgcagg gcgagcacgt ccttggacag gtcgacgccc
     2461 tggtccttct tgaactccgc gatgatgtag tcgatgatgc gctggtcgaa gtcctcgccg
     2521 cccaggaaag tgtcgccgtt ggttgacagc acttcgaact gcttttcgcc gtcgacgtcg
     2581 gcgatctcga tgatcgagat atcgaaggtg ccgccgccca ggtcatacac ggcgatcttg
     2641 cggtcgcccc gctcctgctt gtccaggccg aaggccaggg ccgcggccgt gggctcgttg
     2701 atgatgcgct tgacgtccag gccggcgatc cggccggcgt ccttggtggc ctggcgctgg
     2761 ctgtcgttga agtaggccgg cacggtgatc acggcttcgg tgacgggctc gcccagatag
     2821 tcctcggcgg tctttttcat cttgcgcagc acctcggcgg aaatctgcgg cgacgcgagc
     2881 ttcttgccgc gcacctcgac ccaggcgtcg ccgttgtcgg ccttgacgat cttgtagggc
     2941 atgaggtgga tgtccttctg gacttccttt tcctcgaact tgcggccgat caggcgcttg
     3001 accgcataca gcgtgttctt ggcattggtc actgcctggc gcttggccga ggcgcccacg
     3061 aggatctcac cgtcctcctg gtaagccacg atcgacggcg tggtgcgcgc accctcgctg
     3121 ttctcgatga ccttgggggt gttgccttcc atgatggcga cgcacgaatt cgtggtgccc
     3181 aggtcgatgc cgatgattct tcccatgttc tttctccttg cttgcggccc tgtgcaggcc
     3241 aattcagatt cgatgtgggt tagttgtgga taagctgtga gcttttcaag tgactgtcag
     3301 cccggcctga tgcgggatgc atgcattcat ttcggcgccg ccacggtcac gagagccggc
     3361 cgcagcacgc ggtcggcgat caggtagccc ttttgcaaaa cgctgaccac ggtgttggct
     3421 tcctgtgtgg aatcgggggg caacgggacc accgaaatgg cctgctgttg atgagggttg
     3481 aacttcgcgc cgacggccgg gttgatttcg accaccttgt tgcgctccag cgcgctcttg
     3541 agctggcgca aggtcgcttc cgagccctcg cgcagctgct cgcgcgtggc gtcctcgtgc
     3601 gcgaaactgg cctccaggct gtcgaccacg ggcagcaggc tttctgcgaa gctctccacg
     3661 gcgaatttgc gcgccttgga aatttcgtct tccgcccggc gccgggtatt ttgcacgtcg
     3721 gcctgggcgc gcaggaattg ttccgcgagt tcggcattct tggcctgcat gccggccagg
     3781 tcggcctgaa gctggcccag caggtcgttt tcattggcgg ccttggcggc ttcgcgttcc
     3841 tcgggactca tgtccagggc ggtgggcgcc tcgggccgcg ccggctcgct gggattgttg
     3901 tcggacatct cggtctggtt ctcgtcggac atggcttgtt ctggtggaaa tgaagggctg
     3961 cgcagcagct tggggccggc aagccctttt caagtcgggg agataacgat caactaacta
     4021 agtgcgtccg cccaggcctc ggcccagcgg ttgagcggcc tgaggtgcct caccagctcc
     4081 agccccgacg gggtgaggcc gtaaccggct tgcgccagct cgaccaaacg ggcttcgcgc
     4141 aactccttga gccgggtgtt gagaacggac ggcgaaatct ggtcggcact cgcgcgcagc
     4201 aaccggaagc tctgcggatg gccgtcgcgc agctcccaca gcaagcgcag gctgccgcgc
     4261 cggcccagca ggtcgagcag cgccatgacg ggccggccgg tgcgcgagcc gcgcacggtc
     4321 ttgccggaca ggggggagga aggggtcatg gccggcgatt ctagcgttgg cgccttgtcc
     4381 cgtttgtgac tgccgctacg caatccgtag ccttatattg aggccgctac ggaatcagta
     4441 gcgtatcctc acgccgatcc ggagaaatca tgtccgtcac acccctgccg ccccgaatcg
     4501 aaccgctgtc accgccctac acccccgagc tggaagcctt gctacagcgc atgacgccgc
     4561 ctggagcgcc ttccatcctg gcattgttcc gggtgttggc tcaccacccg gtactggccg
     4621 agcgcatgag cggcttcggc agcctcctgc tcggacgcaa ggccagcctg agcctgcgcg
     4681 atcgcgaggt cgtgatcgac cgcgtctgcg cgcgctgcgg tgccgagtac gaatggggcg
     4741 tgcacgtcgc cgcgttcggc gaggcagccg gcttcacgcc ggcgcagaac acggcgattg
     4801 ccgacatcga ttcgaacgca gcgccgctca cgccgcgcga tcgcctgttg gttcggatgg
     4861 tcgacgaatt gcatgagagc ggcaccgtga gcgatgcgct ctggccggaa ctggcgtcgc
     4921 tgtggtcgac gcagcaactc ctggagctgc tggtgctcgc cggctggtat cacgcgatcg
     4981 gctacgtctg caacgcggcg cgcgtgccgc tggagccatg ggccgcgaaa tgggacggcc
     5041 tgaccccccg ggcctgaccc gcgctatttc aatgcgcgtc gagcagctcc acgtcgaact
     5101 tcagggtggc gttgggcggg atggcgccgc cggcgccccg ggcgccgtaa ccgagctgcg
     5161 gcgggatgac gagggtacgc ttgccgccga ccttcatgcc ggcgacgcct tcatcccacc
     5221 ccttgatcac catgccggag cccagcgtga aagcgaaagg ctcgttgcgg tccacgctgg
     5281 aatcgaattt cgagccctgc cggccgtcgt tgaagagcca gccggtgtag tgcacatgaa
     5341 catgctggcc cggcttggcc tcggcgccgg cgcccacggt cgtgtcgtcg tactgcaggc
     5401 cggaaggggt tgtgatcatg atgggatgaa cctttgctga tgaaaaagat gttggccccg
     5461 cgcgcccctc gcgtcggcaa cggcgggcaa gcgcggcttt aaccgagcag cccgcgccgg
     5521 gcgaggtcgc gcatgagcgc ggccgcgccg taggtccacg gcgcaacctt gtcgctggtg
     5581 ttaacccggt tcgccagtgt accgagccgg ggcgtggaga ccgtaaccag atcgccgatc
     5641 tcatgggtga agccctggcc cggcccgtgg cggtcctggg tgggggcgaa catggtgccc
     5701 aggaacagca cgaatccgtc ggggtactgg tggttggcac cgatggcctg gctcaccagg
     5761 tccagcggat ctcggctgat cttcgcgagc gaactgctgc cttgcatcct gaagccgccc
     5821 cgtccctcga cctgcatcgc caggtcgcag cggcccacgt cggccatgcc gaagtgctcg
     5881 tcgaaaagcc gtatgaacgg cccgatcgcg cagctcgcgt tgttgtcctt ggccttgccc
     5941 aggagcaatg cgctgcggcc ctcgaaatcg cgcaggttga catcgttgcc caaggctgcg
     6001 ccccgcacct cgccgcggct gttcacggcc agcacgattt caggctcggg attgttccag
     6061 ctgctcgacg ggtggatgcc cacttcggcc ccggtgccga ccgagctcat gggctgcgac
     6121 ttggtgaaaa tctccgcgtc cgggccgatg cccacttcca ggtactgcga ccacatgccc
     6181 tgcgccacca ggacgtcctt gagttgcgct gccgatgccg agcccggttg caccgccgcc
     6241 agatcgtccc cgatcaccgc caccacggcc tgccgcacgg cctcggcgca agacgcgtcg
     6301 ccgcgtgcct gctcctcgat gactcgttcc agcatcgaag ccacgaaggt cacgcccgcg
     6361 gccttgatcg cctgcaggtc gcagggtgcg aggaaccagg gcttggcggc gtggcgagcg
     6421 tcgaaagcgc tgttcgccag cacttcctgc gtggcggcga tgcgcgcacc gcggttgtcc
     6481 cgcacgctgt cagccaggtc cggcaactcc agcaattcgc tgcaggtggc ggccagcccc
     6541 gagaggtcgt acacgccgtc gggccgcacc tgcaccagca tcgggccggt gccggcgata
     6601 tgcacccggc cgacgagcag cgcggcgtcc gcgtccgcgg gcaaggtggt ctggatctgc
     6661 aggtattgct tcatgatttt ttcttggctt ggctggcgac ccatttggtc gcgaatgcgg
     6721 gcagctcgcc caggcgcttg agcaggtcca ggccggccgg cgtgacgacg tagccgtcgc
     6781 tgccgtggtt gacgaggccc gcttcgcgca gttccttgat gcgggtgttc agggtattcg
     6841 gtgtgatgcc gccgacgctg tcctgcagca ggcggaaggt ctgcgcatgg ccgtccctca
     6901 acgcccagag cacgcgtatc gcgtaccgcg attcgagcag ccccagcaat tggctgatgg
     6961 cggcgttttc cttcgaaccc atcgtattgt tctcctcggg ccggcggctt tcctgacggc
     7021 ggggagtgga gtgtacgcag ttcctgctac ataaacaata gcagggttac ccccgggcga
     7081 ggcgcggttg ctactttttt tgtggctgaa gggcgcgcgc cgcgggttga tcgcgctcga
     7141 ggtagacctt ctggagcagc ctgatcagcg tcttcgattc ggccgccgtc agccgggacg
     7201 ccacctcggt ttccagttcg gccgccgttc gctccgcgtc gcgcatgagt ttctggccgg
     7261 gcccggtcac gtgcaggccg agcgcgcgtc cgtcgcgcgg atggggcttg cgcatgatga
     7321 tcttgcgttt ctccagcgcg ttgatcattc cgaccaggtt aggcggcagg atgcccaagg
     7381 tggcgcacaa ctgtcgcgag gtgatgccgg ggttgtgcat gatgagggag agtaccgaga
     7441 agtccaccgg ccgcaggtcg tagaccttca tgcgctccag gaacacatcg atcaccgcga
     7501 gcgccgcgcg ccgggcgttg tagccgatca ggccgtcgag atacccggtg tcgacctcct
     7561 ccacagccgg ggtgttgcgg acctgcaggg tcttgagggt tctggcgatc atggcgcgct
     7621 cacggatttt tggacatatg ctgccacagc cggcgcttcg ggcctcgaag aggccgccgc
     7681 gcactggccc tggatgatct tgatgcgcat gtcgaattca ggcagcacgc gcaagcgcag
     7741 tgcggcggca ctgcggctcc agcgcggcac gccggcgggc gcggccgttt cgatgcgggt
     7801 ccacgcccag gccagcaagg ccagcgcgag ggcgcgcagg tagtcatcgg ctgcttcgaa
     7861 cggcagcacc gggtcatgaa gggctgcctg cgccaaccgg gccgtcaggt cgcgcagttc
     7921 ggcgaaacgg caaaggacat cggcatgggc cggtcggctc gcatcgaggt ccgcatccag
     7981 ccccgacagg agcgccgcca ggccggcgcc gccgtcggca agcaccttcc ggaccaccag
     8041 gtcgatcgcc tggatttcat tggtgccctc gtagatcatg gcgacacgcg aatcgcgcac
     8101 gatctgctcg atgccccatt ccctcacgta gccgtggccg ccgaagacct gcaggcaggc
     8161 gctcgcgccg tgaaatgcct gctcggtcaa ggcggccttg agcaccggcg tgaccaaggc
     8221 gcaccagcgc tgcgcgctcg cgcgccgctc ggcctgcgga tggtgcttcg cgatgtcgag
     8281 ttcgagcgcg gtctggtagg ccagcacccg gccgccgtcc acccaggcgc gctgcgtgtc
     8341 gaggatgcgg cggatggcgg gatggccgag aatcgcgtcg gccgcctcct tgccgtcgcg
     8401 ccgaccgggc acgcgcatct gccggcgctc gagcgcatag gcctcggctt tctgccaggc
     8461 cgcgtcgagc aggccgaggc cctgcagcgc cacatgcagc cgcgccgcat tcatcatcac
     8521 gaacatggcg ttgaggccgc tgctggtttc gccgaccagc cagcccgtgg catcttcgaa
     8581 acgcatcgcg caggtggggc tgccgtgcag gcccattttt tcctcgatgc ggtcgcagtg
     8641 caccccgttg cgcgtgccgg ccggcaggac cttcggtacc agatacagcg agagcccctt
     8701 gggtccggcc ggcgcatcgg gcgttcgcgc gagcaccagg tggacgatgt tgtccgtgag
     8761 gtcgtgttcc ccgccggaga tgaagatctt gctgccgttc aggcgccagc tgccgtcggc
     8821 ctgcgccatg gcgcgcgtgc gcaccagtcc caggtcgctg cccgcgtgcg cctcggtcag
     8881 gcacatcgtg gccagccact cgccgctggc gatcttgccc aggtagcgcg ccttgaggcc
     8941 ctcgctgcca tggtggcgca ggcattcgta ggcgccatgc agcagcgcgg gcgccatggt
     9001 ccagccatgg ttggcgccgc tgagcatctc gtagagcgcg gcctcgagca cgcccggcag
     9061 cccctggccg ccgtccgccg gcgcgcaggc cagcgcgggc cagccgcctt gccagaacgc
     9121 ctggtacgcc tcgcgaaaac cgggaggcgt ggtgacgtcg ccgtcctgcc agcggcagcc
     9181 cggcgagtcg ccggcgccct gcaacggcgc cacgacttcg gccacgaact tgccggcttg
     9241 ctcgatgacc tgcgacatca gttcgcgatc ggcctccgcg aaggcgggca tcgccgcgag
     9301 ctgcgcgtcg gcgcgcagca cgtcgaacag catgaaacgg atgtcgtcct gggcggggtt
     9361 ccagttcatg aggtgtacta atcgtccgcg tgaatcgcgg cccccaggta cgtatcgatc
     9421 acgcgcgggt cgctagcgag ttcctgcgcc gatccctcca gggcgatctc tcccatttcg
     9481 agcacgtagc cgtggtcggc cacttcgagc gccgcgcgcg cgttctgctc aaccagcagg
     9541 atcgtgacgc cggtctggcg caggctgtcg atggtgcgga agatatcgag cacgatcagc
     9601 ggcgccaggc ccaggctggg ctcgtcgagc atcagcaggc gcggccgcga catcagcgcg
     9661 cggccgacgg cgagcatctg gcgctcgccg ccggaaaggg tgccggccag ctgcagccgg
     9721 cgctccttca ggcgcgggaa gatcgcgtac acgtcatcga tgcgcgcgcg ccactcgcgg
     9781 ttgcccaatt gcacctgccg gtagccgccg agcaccaggt tgtcctccac ggtcatggag
     9841 ccgaacagct cgcgccgctc gggcaccagg gccatgccca tcatgactcg ctcttccagc
     9901 ggcatgacgc cgatgtcctg cccatcgaat tgcagcgtcc cgcgcgaggg gatcacgccc
     9961 atcagcgtgt tcagcagcgt cgacttgccg gcgccgttgg gaccgatcac cgtgacgacg
    10021 ctgcccgggg ccgcctccag ggccaggccg tgcagcacct cggcccggcc ataaccggcg
    10081 tgcaggttgt cgatgcgaag catgggagtg gtcatggcaa cgccttagtg ttcggtaccc
    10141 agataggcgg cgcgcacctc ggggcttcgc tgcacttcgc gcggcgtgcc ttccatgagg
    10201 agcgtgccga actccatcac caccagccgg tcgcacacgt ccatcaccag gtccatgtcg
    10261 tgctccacca gcaggatgct catgccctcc cgctgcaact ggcgcagcac gtcgatgagg
    10321 gcgcgctttt ccttgtggcg cagtccggcg gccggctcgt ccagcagcag cagcgccggg
    10381 tcggagcaca gggcacgcgc gatttcgagc agccgctgct gtcccatcgg gaggttgccc
    10441 gccagttcgt gcagccacgc tcccatgccg atccgctcga gctggcgctg cgcttccttg
    10501 aagagggcgc gctcctcggc gcgatccagc cgcgccatcg cggcgagcac gtccttgcgc
    10561 ccgcgcgcgt gggcgcccag cgcgacgttc tccagaaccg tcatgtcggg gatcatcttg
    10621 acgtgctgga aggtgcggcc gagcccgcgg cgcgcgatcg cgcgggcccg ctggccgccc
    10681 aggctttcac cgcggaacgt cacggcgccg ccggtcagcg acagcacgcc cgtgatgagg
    10741 ttgaagatgg tggacttgcc cgcgccgttg gggccgatca ggccgacgat ctcgcccgcg
    10801 cgcagctgga agctcacgtc gttgacggcg accaggccgc caaattcctt gcgcaccgcc
    10861 cgcacgtcga gcagcagttc accggacagc ggtttgggct tttccggcag ggcaggcgca
    10921 tcggcccaat cgacggtccg gtcgcggcgc ggcaggcggc gtgcgacgaa atcccacagg
    10981 ccatcgggct ggtacttgag catcaggacc agggcgacgc cgaatacgat cacctcgtag
    11041 ctgccgctgg ttccgatcag gcgcggcagc agcacctgca gctggtcgtc cagcagcttg
    11101 gtcagcgcgg cgcctgtgag cgcgccccat acatggccga ccccgcccac caccgtcatg
    11161 aagaggtact cgatgcccat cttcaggccg aaggaggtgg gggtcacggt gcgctggaaa
    11221 tgcgcgaaca gccagcccgc caccgaagcg aacagcgcgg cgatcaggaa gatgatgacc
    11281 ttgtagcgga atgtgctgat ccccatcgac tcggccatct gcgagccgct gcgcagcgcg
    11341 cggatggcgc ggccggcgcg tgaatcgagc aggtgcagca gcgcggtggc cagcgccagc
    11401 acgatggccc aggtcaacac gaaaaatccg cgcccctgcc cgatgtcgag cccgaagaag
    11461 gtgaggctct tgatgcccag gatgccgtcg tatttgccga gcgcgtcgat gttgcccatc
    11521 aggtagtaca gcgacaggcc ccatgcgatg gtggccaggg gcaggtaatg gcccgacatg
    11581 cgcagcgtga tgagggccag cagcagcgcg ctggcccctg tgatcgccag gccgatgggc
    11641 agcgtgagcc agggcgagat gcccgtgttc accgtgagcc aggcggtggt gtaggcgccg
    11701 atgccaacga acgcggcttg tccgaaggag gtcaggccgc cgatgccggt cagcagcacc
    11761 aggcccaggc aggtcaggct gtacagcccg atgtaattga gctgcgtgat ccagaaatcc
    11821 ggcagcggga gcagcgcgac cagcggcacg gcggccgcga gcgcgatcag gcccagttgc
    11881 cgcctggaga gcagggggcc ggtggtcgcc atgcgggcaa tcgacacagg ttcgctgttc
    11941 atgcttcgtc gtccgcgtga acaccgtaaa gggaacgcac cagcaggacc ggaaggatca
    12001 gcgtgaagac gatcacttcc ttgaacgcgc tggcccagaa cgagccaaac gactcgacga
    12061 cgccgacgaa cagcgccccg aggagggcgc cgggatagct cgacagcccg gccattaccg
    12121 ccgccacgaa acccttcagg ccgatgatga aacccgagtc gtagaacacg gtggtggtcg
    12181 gcccgatcaa caggcccgac agcgcgccga tcagcgccgc catggcgaac gtcatctggc
    12241 cggcggactg cggcgagatg cccatgagcc gcgcgccgag ccggttcaca gccgtcgcgc
    12301 gcagggcctt gccgcgcagc gtgcgttcga agaacagcca gagcaggacg atcagcgcga
    12361 tcgaggccag cgcgatgatg atggcctggc cggtgaggtt cacgatcccg agcgaaatgc
    12421 gcaggtccca gaacgacggg ttgcgatagc cttccgcgcc gaagaacagc aggcccaggc
    12481 ccgtcatggc gaagtgcacg ccgaccgaga cgatcagcag caccagcggg ctggcatggg
    12541 cgagcgactg gaaggccacc cggtaaacca gcggaccata ggccgtgacc accaggacgc
    12601 tgagcaatgc ctggatggcc aggggcggct ggtgcggcgc cgcgaacgcc gtcaccgcgg
    12661 ccgcggccac gggaaacgcc agcgtgcgca ggccggactt cagcgccaca agcaggtggc
    12721 ggtggcgctg ccactcgccc cagacctgca tcagcgccgc cacggcggcc aggaccagga
    12781 gcagccacac gctgcccggc accttgccgg tctgcaggat cgcaagcgtg agggcgccgt
    12841 aggcgacgaa ctcgccttgg gggatgaaga tcacccgcgt gacggtgaac accagcaccg
    12901 tggccaggcc cagcagtgcg taaatcgcgc cgttggttgc gccatcgatc agcaggacgc
    12961 tggcaatcga caaatccatg tcagtgatcc tttgcgtttc gtgccacccg cgacccatga
    13021 agtcgcgctg tcagtgcggg ggccaccgcg gaaccggctt tgccgggccg ctggtggcgt
    13081 ccccttgagg gggagagggc gaagcccgag ggggcatcgc cgaaggcggt tcgggggtgg
    13141 gccgttactc gaccttgaat tcgccgttga cgaccttgag catcacgccg gtttcattgg
    13201 tgaagcccca gtggtcgttc ttggaccagt tcatcacgcc gtgcgagaag gtcgtgcggc
    13261 ccatgttctc catggcctcg cgaagcgcgg cgcggaactc cttggtgccg ggcttggctt
    13321 tcttcagcgc gaccggcagg atcttttcga gcacgatctg cgaatcgtag gcatgggcgg
    13381 cgaactggtt gcgcgtgccg ggtcccacca gcttctcgta cttctggacg aaatcgatcg
    13441 cgagcttctt ggacgggtgg gtctcgggca gctgctcggc gatcacggcg gggccggata
    13501 ccacataggc gccttcgaca tccttcccgc ccacgcgcat caggtccttg gtggccgccg
    13561 catgggtctg gtagatcttg cccttgaagc cccgctcgac gatcgccttg tgcggcatcg
    13621 cggcgccgct tccggtggcg acgatcagca tggcatcggg gttggccgac acgagcttaa
    13681 gcgcttgcgg cgtgacgctg gtgtcggggc gcgcgaagcg ctccaccgcg atcaccttga
    13741 tgccggcctt ctcggcgatg ggcgtgaact ccttgagcca ttgctcgcca tacgcgtcgg
    13801 tgtagcccag gaagcccacc gtcttgacac cctgcttggc catgtgggcg accatgggat
    13861 gcgacatgac cgtgttcgac tggggcaggc ggaacatcca ggcgtccttg cccgggggca
    13921 cgcccgccgg cgaggcggcc agctgcaccg tcccggattc agccgcgact tccgccatcg
    13981 cggcggccac ggtggtcagg gtggaaccga tgatcatgtc caccttgtcg tcggtgacga
    14041 agcggcgggc gttgctcacg cctttgcccg gatcgctggc gtcgtcgagg atgatcaggt
    14101 tcaccttctc gccggcgatg gtctgcggga acaacttgta gtagttctgc atcgggatgc
    14161 ccaggccgga gcccggaccg gtgagcgaga ggctcacgcc gatgttgacg tcggccagcg
    14221 ccgcggccga ggcgaccagc gtcgcgatcg caatcagtgt cttgtggaat ttcatgcagt
    14281 gtctccagtt gtggtgggtg gaaaaaaaca ataatgatgg gtcgggccag actgacgcgt
    14341 tcagccggtc ctgaccagcc cccgcggctg gatcgtgaaa ggcaaggtgc ccgcatgcag
    14401 cgcgttgacc agggcctcgc ggtgctggag cacggcgcgc tggttgatcg agcccttgtc
    14461 cgtcgcttcg cccttgtcaa tcgatggggg ctcgtgcatc aggtgcatcc gggcgacgcg
    14521 ggtggcgctg ccggtggcgc tccccgccag ctcgtcggcg acccgctgga aatgcgcctg
    14581 caccggcgcg ctttccagca cgtctttgag cggggcatcc gcgggcaggc cggccagctt
    14641 gcgcaccgcg gcggtaggga tgaccagggc gccgacttcg tgcaggttga tgcccgtgat
    14701 gacggcgtcc tgtatgtaag gcgccccggc ggcgatgatc ctcgcccgca agggtccgac
    14761 actgacgaag gtgccggtcg cgagcttgaa gtcctcggcg atgcggccgt cgaacttcag
    14821 cccctggtgc acgtcgtccg ggtcgatcca cagcacggcg tccccggtgc ggaagaagcc
    14881 ctcctcgtcg aacgtctccc gcgtcgcggc ctcgttgcgc cagtagcccg gcgtgatgtt
    14941 ggggcccttg tagcgcacct cgatcttgcc gtccgtcggc accagcttga gctcgagtcc
    15001 cggagtggga acgcccaggt agccggcctt cacgttggga ttggtgatga agacggcgaa
    15061 cggcgccgat tcggtcatgc ccaggcccgt gcccatgacg atgcgctcgc cgatctcgcg
    15121 ctcctgcacc tcgtgcaggg tgtcccacac cggctgggcc agcgccgcgc cggcatagaa
    15181 aaacattctc acgcgcgaca gcaggttgcg gcgcagcaca tcgtccgtct tcatcgcgtt
    15241 ggcgatcgcc tcgaaaccgg tgggcacgtt gaagtagacg gtgggcgcga tctcgcgcag
    15301 gttgcgcagc gtctcgccga tgagggccgg caccggcttg ccgtcgtcga tatagagcgt
    15361 gccgccgttg tagaccgtca ggccgaagtc gtggttgccg ccgaaggtgt ggttccacgg
    15421 cagccagtcc accagcaccg gcggttcctc ggccaggacc ggcatcgact ggcgcatctg
    15481 ctgctggttg gcgcaccaca tcccgtgggt gttgatgacg gccttgggca gcttggtcga
    15541 gccggaggtg aacaggaatt tcgcgattgt ttgggggccg gtcgcctgca tggcggcatc
    15601 cacggcggtt gtggccgtgg tggccagcag agccgcgaac ggtgtggtgc cgcgctcttc
    15661 cagcgcgccg ttgccgagga cgagctcgac ctccggtccc accgtcgccg cgatggcctt
    15721 gccgtagcgc gcggcgtccg ccgcgaacac cagccccggc gtcaaggtcg cgagcgcatg
    15781 gcgcagcttc tcatggtcct ggctgatggt ggagtaggcg ggcgacacgg ggcagaaggg
    15841 tacgccggcg tagaggcaac ccagcgccag caatgcgtgc tccaggtcgt tttcgctgag
    15901 gatggccacc ggacgttcgg cgttcaggcc gcggtcgatc agcgcctggc cgatccgccg
    15961 cgcgctgtcc agcgcttcgc tgtaggaaat gcggcgccag tcgccggtgg tgccgtcggc
    16021 cttgcgcacg cgtcgcgcca tgaagatgcg gtccggcgcg gtgcgggccc agtgcaccag
    16081 gcgatcggtc agccgcggcg cgtgctcgcg cagcggctgc tcggccttca tgtaacacac
    16141 cccgccggcg ctttcgcgca ggatcacccg gctcacggga aactccaggg cccggtactt
    16201 cggcgagctc atttcatgct ttcatggctc aggcgcgcga tggcggcctc gtcggcgaaa
    16261 tggagaaatt tcgtactcat aaaggggtat gccaagtggc gcgattatgg ttataaacaa
    16321 taaccaattc aagcggattg tcctaggact tacccgtagc tttccaaccc attgccgatg
    16381 gcccgggatc ggctgggcta gccgcccacg cgcaacacca gcgccatgcc ggaatcgccc
    16441 gcggcacatc caccgaacaa gccgacgccg ccaccgcgca aggccagttc ctcgatgagt
    16501 tcgatcacgc tgcgcacgcc ggtcggtccc tgcggatggc cccagatcag cgagcacccg
    16561 tagttgttca tcttttccag gggaaagccg gtttcgagcg cgaatgcgat atcgttcacg
    16621 gcgaacgggt tgtgcgtctt caccgcggcg acgtccttga tgccgagccc ggcatttttc
    16681 aatgccgcca tcgcggccgg cgcgaccgcc atgggcatgt ggcctttttc gaccctggct
    16741 tgcccgaacc cgagcagttc cacctcgatc ttcggatcga gcgacacctc gcgcgcgatt
    16801 tcgcgtgtcg tgacgatggc gccggcgttg ccgtccgccg gatgcgtctg cgagccgaag
    16861 gtcacggtgc cgcccggctt cacgggcttg agcttggcga gcccttccac ggtcgtggga
    16921 aagatgccgt cgtcggccga cagcgtaccg atctgcttgc gaaagcccgc atcggtgatc
    16981 ggcacgtcga ccatgtaacg gcgctggaag gcgcggtcat cggccagggc ctgccggtat
    17041 tgcgcgtaac gcgccagggc cacctcattc tgttgcgagg tgccgatgct gaactttgcc
    17101 gccacgtttt cggcggtgtc gatcatcgcg ttcttcgcat gcgggtcgtg cgcgaagttg
    17161 tccagcaccc agcgctcggt gtgtccggtg cctcccggcg cggtcggatc cgggtaatac
    17221 acgatcgggc cgttcgagca gcggtcggtc gtgaccagca aggcgcacct ggatgaaccg
    17281 tcggccacct cggcggcggc catttgcagg cctcgggcgc tggtggcaca agcttgctgc
    17341 accgtggggc cggcgacgtt ttccaggccc atcatcccgg tgacataggg caggccgtag
    17401 aaactgccat gctgcgggtt ggtgtggccg aggatcccga gatcgatgcg ttccatcgga
    17461 aagcgtttgg cttccaatgc ctgcctggcg gtgttcgcgg ccagtcgcag gctgttcaga
    17521 tgggcgatgg ggccttgcca tttggcaaag ggggtggacc agtaaaggcc gtagggaatg
    17581 tatgcgtgag ccatgatgtg ttcctgaaat caacgtgatt gttcggtttg ccattgcccg
    17641 aaactcgagc ccgaaagcgc cagttttttc agcagcggcg ccggctgcca gtactgcgga
    17701 tcgagcgttt tctggaactc catgatcttg tcgtagacca ccttgagtcc caccgtgtcc
    17761 gcgtagaaca tcgggccgcc gcgataacgc ggaaagccgt aaccggcggt gtacacgacg
    17821 tcgatatcgc tggcgcgcag ggcgatgcct tcttccagca acagcgcgcc ttcattgatc
    17881 atcgcgtaca ggcagcgctc ctggatttcc tgcgcgccgg gattgcgctg cgggatgtcc
    17941 aggcgcttgg cctcctcgcg cagcagggcg atcacctccg ggtccggcgt gcgcttgcgc
    18001 tcggcgccgt cgtagcggta atagccgcgt cccgctttct ggccgagcca gccgtgttcg
    18061 tagaggaccg ccgtgggccg gtagaagctc gggtcctgcg gcagcaggtg cttgcgttcg
    18121 agccgcgtca ggtagccgac gtcgacaccg gccatgtcgt acacggcgag gatccccatg
    18181 gccatgcccc attgctcgag cgcgccatcg acttcggccg gtgtcgcgcc ctccagcacg
    18241 cagcgctcgg cttcgcggcc gtacgggtcc atcatgcggt tgccgatgaa gccgtagcac
    18301 accttggcca cgacgccggt cttcctgatt cgcctggcca gttccagcgc agtgaccacg
    18361 acatcggggg cggtcttgcc ggcctggacg atttccagca gctgcatgac gttggcgggg
    18421 ctgaagaaat gcagaccgat cacgtcctgc ggccgccggg tgacgcccgc gatctcgtcg
    18481 atatccaggg tcgaggtgtt ggtgccgagg accgcgccgg gcttggccac ggcatcgagc
    18541 ttgccgaaga tctccttttt cagggcgagg ttttcgaaca cggcctcgat gatcaggtcg
    18601 gcatcggcca gcgccgcgaa atccaggctc ggcgtgatca gcctgatgcg ctcctcgagc
    18661 tgctccggct tgaggctgcc gcgcgaaacc gaacgttcgt agttcttgcg gatcgtcgcc
    18721 atgccgcgat cgagattctg ctgcgcggca tccaccagcg tgacgggaat tccgacgttg
    18781 acacagcaca tggcgatgcc gccgcccatg gtgccggcgc cgacgatgcc gacgcgcgcg
    18841 atggggcgcg gcgtaatgtc aggcgcgagg ccggggatct ttcgcacttc gcgctcggcg
    18901 tagaacaggt gacgcagcgc cttggactcg gtggcttgct cgagctggga tgacaactcg
    18961 ctctccacct gcaaaccctg gtcgaaaggc ttttccacgg cggcctgcag cgccgtgatc
    19021 agcgcgagcg gagattgccg gttgcgcatg gtcctggcga cctgggcgcg cttggccgcg
    19081 aacagttccg cggcctgttc cgcccccttg accggttgct cccgggtgcg gcgcggaccg
    19141 gcgccgccgg cgaccagttt gcgcgcgtag gccaccgccg ccgcgaccac ctcgttgccg
    19201 acaaccagat ccgccagccc cgcttcggcg gcctttgctg ccggcaacgg cttgccggtc
    19261 agcgccatgt cgagcgcgac gtcgagcggc acgatgcgcg gcaggcgctg ggtgccgccg
    19321 gcgccgggga tgatgccaag cgagagttcc ggcaatccga agcgcgcccc ttcatgcgcg
    19381 acgcggtagt ggcaggcgag cgcgatttcc gtcccggccc cgagcgcggt gccgtgcacg
    19441 gcggcaacca ccggcaccgg gctgtcctcg atcatgcgca ggacctcgtg atagccgggg
    19501 ggcgcgatgc cggtatcgaa ttccttgatg tcggcgccgg caacgaaggt cttgccggcg
    19561 catccgagga cgatggccct ggttcccggc agtccccgca attcggcgaa tacccgtttc
    19621 agtcccgccc gtacgctctg gttgactgca ttgacgggag ggctgtcgat catgacgacc
    19681 gcgacgtctc cggtcatgga ataactcact gcttcgctca cgcttgttct ccttgtttca
    19741 ggtcgatttc ggattcagct ggatgcgccg tcgatgcacc gcaactgggc ggtggcgtta
    19801 cttttccgtt catgtcgggt ttgccaggcg gcgcgattat ggttatattt gataaccaat
    19861 tcggccggat cggcctaggg cttaccctag tctcctgtca ccaaattccg cgagacacat
    19921 ggatcaccag aacctcatcg ctatcgacgt tcacacccac gctgaagtca gctgctggaa
    19981 ccctttcgac aactacggcg aggagtacga ccgcgccgcg gacaagtact tcggctcgaa
    20041 ccgccggccc accatcgccg aaaccattgc ttactatcgc gaacgcagga tcgggctggt
    20101 gatgttcact gtggacagcg agtcgcacct cggccgcagg cgcattccga acgaggagat
    20161 cgcgcaggcg gccaaagaga acagcgacat gatgatgtgc ttcgccagca tcgacccgca
    20221 caaggggaag atgggggcgc gcgaggccga gcggctgatc agtgaacatg gcgtgaaggg
    20281 cttcaagttc cacccgacgg tgcagggcta ccacccctac gacaagatgg cctggccgat
    20341 ctacgaagtc gtcaacgcgc acaagctgcc cgccatcttc cacaccggcc acagcggcat
    20401 cggggcgggc atgcgctgtg gcggcggctt gcggctggag tacagcaacc cgatgcatct
    20461 ggacgacgtc gccatctcgt tccccgacat gcagatcgtc atggcgcatc ccagcttccc
    20521 gtggcaggac gaagcgctgt ccgtggccac gcacaaaccc aacgtctgga tcgacctgtc
    20581 gggctggagc ccaaagtact ttccgaagca gctggtccag tacgccaaca cgctgctcaa
    20641 agaccgcgtg ctgttcggca gcgactatcc actgatcacg ccggagcgct ggatgaagga
    20701 cttcgaggaa gccggcttca agcccgaggt gatgccgggc atcctgaagg gcaacgcagt
    20761 gcgcctgctc ggcctgggct gaagccctgc ctcacggctg ccttcagtag cgcagctgcc
    20821 ccgagctgtc gatgatcgcc agctccacgt aggaagagcc gttgtgggac tgcggtgagt
    20881 agctggcggt gaagcggcca tatcggacct cgcccgcggc ttccatgccc ttgatgaatg
    20941 atgcggtcgt gaggttcttg ccgccgcgac gcagcccttc caccagtagc ttcgcgtgca
    21001 cgaagccctc gaactgcgaa gcggacggtt tggcgccggg ggtcttttcc gccagcagct
    21061 tctggtactc gaggaccacc gggatcgatg tgttcttgag cgaaggcatg atctgcgaaa
    21121 gcacgatgcc ctgcgctccc gatccgagtt ccttgttgat cacgtccagg ctggcaaccg
    21181 agaatccgta gaagctcggc ttcacgccgg tctgcctcat cgccttgacg aagttcggaa
    21241 aggtcgtgcc ggcggtgcac atgatgatga cttgtggctc cgctttttct gccgcagccg
    21301 cggcggcatt gaagtccggc tgcttggggt cgaccttggc ttcggccacc agctgcagct
    21361 tttccttttc cgccgccttg cggatctcac cgagcaacgt cttgccgaat gtgtcgtcct
    21421 ggtagaacgc cgccacccgg gtgacgccgg tctgcttgag ctgcgagacg atgcgccgcg
    21481 cctcattggc gtaactggcg cgaacgtgga acaggtagcg gttgaacctg tcccggaaga
    21541 tcgacgcgcc ggtaatcggc ccgaagacga tggtccggct gtcctccatc atcggcagga
    21601 tggccgccgt atgcccggtg cccgagttgt tgtagatcag gaaaaccttg tcttcctcga
    21661 tcagcttgcg cgtgttttcc agggccttgc cgacgtcgaa ggcatcatcg agcgtcgtgt
    21721 agacgatctt gcggccgtaa acgcctccct tgtcgttgat cgaatcgagg tacaggcgcg
    21781 aacccacgcc gtattcgcgt gtctgcggcc cgagcggacc gctgagcaca gccgacgcac
    21841 cgatgcggat ttccctgggc atgacgcccg cgtcgccggc atgggcgccg cccgccagcg
    21901 cgccaaggag cagcgcggga aaaaccagct tctgcattgc cattaccttt cagtcaaagc
    21961 gggagcgcgg atggattcgc gctatcgccc ggcactcgag cgtcaccgcc atttagttat
    22021 gaaacatcat caatgtagtg ctacacaata actattgggc ccggtgcttt ccctaagggg
    22081 ccccaacaaa tggctagacc tcgagtgcgc tccagcattt ccaatggcct tccccatcga
    22141 aaggaaagcc gtgaaagctc aagccaaccc caacaagcag ctcctgcagg acgtcttcgc
    22201 cggtctcgcc gaagcgcgaa tcacggcatg tactggccgc cgtggccgtc gtcggccatg
    22261 gcggctcctg aaaagcgaag agggaaaatt agcgcgccag cagcaggccc ggcagccaca
    22321 gcgaaatcgc
//
LOCUS       DASWIY010000383         2828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_633325, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000383 DASWIY010000000
VERSION     DASWIY010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633325"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2091)
                     /locus_tag="VGA59_13700"
     CDS             complement(<1..2091)
                     /locus_tag="VGA59_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9720775.1"
                     /translation="MNARPSCPILLVDDQAANLLALEAVLQGLGEPLVRANSGQEALR
                     RLRDEDFAAVLLDVRMPGMDGFETARLIRAQRRSRATPIIFITADATDLSVEEAYAIG
                     AVDLLTKPLVAAVLNAKVAFFVELHRSRQELQAAERRAVEDRVFLSAVLEAIEDGVVA
                     CGADGVLTLFNRATREFHGLGAQALDADDWAERYDLYRTDGKTPLAKEEIPLFRAFSG
                     EAVRNAEMVIAPKGGKARVVLASGQPLYDAAGRKLGAVVSMHDVTARREAQAASEAAA
                     REQSRRRDAEAAAELIRESRERLRSTEERLRLATDAAGLGVWVWNVADDTETWENERM
                     YALLGLSAQDQPVGGRRFAREFLHPADAGAFKRAMARTVRNNEPLRFEGRFRRHHDHA
                     LRWIELTGIRQAGPDGSPLRVLGTAADITHRKEAEEKLRKSEERYRTLFESMDEGFCV
                     VEMVFDPAGRPCDYRFLEINPAFEKHTGLIGAQGMRMRELVPDHEAHWFETYGRVAAT
                     GEPVRFENEARAMGRWFDVYATRLGGPGSTKVAVVFGDITERRRSRESLERLAAELAE
                     TDRRKTEFLATLAHELRNPLAPISNGLHLMRMASGNPRAQEKAREMMERQLRQLVHLV
                     DDLLDIARISCGKVDLKKERIDLKTVLASAVESSTPLVGAAGHTLALQLADEGLELVA
                     DPIRLGQVVSNLLNN"
     gene            2259..>2828
                     /locus_tag="VGA59_13705"
     CDS             2259..>2828
                     /locus_tag="VGA59_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720776.1"
                     /translation="MNESSARRVILAQAIETADSQGKLLSEVERSQIDREARQAAQAD
                     GAGHAPGVAERFLDLRAQRVLQAMAQRNPAIASLQGVPAWRPWLLVVTPLATLVLGAL
                     TERIADPHRVDLLSLPLLAIVLWNLLVYVFLVVGWFLQPRGRERPLLEATSRWAAGLR
                     GWRRRSGHLRADVTALFHLRWLKATSSVRL"
BASE COUNT          408 a         1011 c          941 g          468 t
ORIGIN      
        1 gttgttcagg aggttgctca ccacctggcc caggcggatc ggatcggcga cgagctccag
       61 gccttcgtcg gcaagctgta gcgccagcgt atgcccggcc gcgccgacca gcggcgtgct
      121 ggactccacc gcactggcca acactgtctt caagtcgatc cgctctttct tcaggtccac
      181 cttgccgcag gaaatacggg cgatatcgag caggtcgtcc accaggtgca ccagctgcct
      241 gagctggcgc tccatcattt cgcgcgcctt ctcctgcgcc cgcgggttgc ccgatgccat
      301 ccgcatcagg tgcaagccgt tgctgatggg cgcgagcgga ttgcgcagct cgtgcgccag
      361 cgtggccagg aactcggtct tgcgccggtc ggtttccgcc agctcggccg ccaggcgctc
      421 caggctttcc cgggagcgcc tgcgctcggt gatgtcgccg aacacgacag cgaccttggt
      481 gctgcccggc ccgcccaggc gagtcgcgta aacatcgaac cagcggccca tggccctggc
      541 ctcgttctcg aagcgaaccg gctcgccggt cgccgcgacg cggccatagg tttcgaacca
      601 gtgcgcttcg tggtcgggca ccagctcgcg catgcgcatg ccctgggcac cgatcaggcc
      661 ggtgtgcttt tcgaaagccg ggttgatttc gaggaagcgg tagtcgcagg gccggcccgc
      721 cgggtcgaac accatctcga ccacgcaaaa gccttcgtcc atcgattcga acagggtgcg
      781 gtaacgctcc tcgctcttgc gaagtttttc ctcggcctcc ttgcgatgcg tgatgtcggc
      841 cgcggtgccc agcacgcgca gcggcgagcc gtcggggccg gcctgccgga ttccggtgag
      901 ttcgatccag cgcagggcgt ggtcgtggtg gcgccggaaa cggccctcga aacgcagcgg
      961 ctcgttattc ctgaccgtgc gcgccatggc ccgcttgaac gctccggcgt ccgccggatg
     1021 caggaattcg cgggcaaagc gcctcccgcc cacaggctgg tcttgcgccg acagcccgag
     1081 cagcgcatac atccgctcgt tctcccaggt ctcggtatcg tccgcgacgt tccacaccca
     1141 tacgcccagc ccggcggcgt cggtggccag gcgcaagcgc tcttcggtcg agcgcagccg
     1201 ctcccggctt tcacggatca gctccgcggc cgcttcggcg tcccggcggc ggctttgctc
     1261 gcgcgcggcc gcctcgctgg ccgcctgtgc ctcgcgccgg gccgtgacat cgtgcataga
     1321 caccacggca ccgagcttgc ggcccgccgc gtcgtacaac ggttgcccgc tggccagcac
     1381 gacccgtgct ttgccgccct tgggcgcgat caccatctcg gcgttgcgca cggcctcgcc
     1441 cgagaaagcg cggaacagcg gtatctcctc cttggcgagc ggcgtcttgc cgtcggtgcg
     1501 atagaggtcg taacgctcgg cccagtcgtc ggcgtccaag gcttgcgccc ccaagccgtg
     1561 gaactcgcgc gtggcgcggt tgaacagcgt caggacacca tccgcgccgc aggccacgac
     1621 gccatcttcg atcgcttcca gcacagccga caggaagacc cgatcctcga cggcgcgccg
     1681 ttcggccgcc tgcagttcct gcctgctgcg atgcagctcg acaaagaagg cgaccttggc
     1741 gttgagcacc gccgccacca gcggcttggt cagcaggtcc accgcgccga tggcgtaggc
     1801 ctcttccacc gacaggtccg tggcatccgc cgtgatgaag atgatgggcg tggcgcgcga
     1861 ccgccgctgc gcgcggatca gccgcgccgt ctcgaagccg tccatccccg gcatgcggac
     1921 atccagcagg acggcggcga agtcttcgtc gcgcagccgc ctcagcgctt cttggccgga
     1981 gttggcccgc accagcggtt cgcccagtcc ctgcaggacg gcttcgagcg ccagcaggtt
     2041 ggcggcctgg tcgtcgacca ggagaatggg gcacgatggg cgggcgttca cggtgcgacg
     2101 ctactacaaa acaccggtcc gcgcgctccg aggaaacgcc acaacctcga tggccgccgg
     2161 ccgccggcac ctgcaaccgg cgcattggcg gccatgaacc gatgcgcgag gcggcaacat
     2221 aatggcggaa tgcacaagtt gccccggccc gcatgagcat gaacgaatcc tcggcgcggc
     2281 gagtcatcct ggcccaggcc atcgagacgg cggactccca gggcaagctt ctgagcgagg
     2341 tggaacgcag ccagatcgac cgggaggccc ggcaagccgc gcaggcggac ggcgccggcc
     2401 acgcgccggg cgtcgccgag cggttcctgg acctgcgcgc gcagcgggtc ctgcaggcca
     2461 tggcgcagcg caatccggcc atcgcgtcgt tgcagggcgt ccccgcctgg cggccctggc
     2521 tgctggtcgt gacgccgctt gccacgctgg tcctgggcgc gctcaccgaa cgcatcgccg
     2581 atccgcaccg ggtcgatctg ctgtccttgc cgctgctcgc gatcgtcctg tggaacctgc
     2641 tggtttatgt cttccttgtc gtgggctggt tcctgcagcc gcgcgggcgc gagcgcccgt
     2701 tgctcgaagc cacgagccgc tgggcggcgg gcttgcgcgg gtggcgccgg cgatcgggcc
     2761 atttgcgagc cgatgtcacg gccttgttcc acctgcgctg gctgaaggcg acctcatccg
     2821 tgcgattg
//
LOCUS       DASWIY010000384         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_263697, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000384 DASWIY010000000
VERSION     DASWIY010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263697"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..1461
                     /locus_tag="VGA59_13710"
     CDS             325..1461
                     /locus_tag="VGA59_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX9720777.1"
                     /translation="MATKMQGEQPGRPGRKPSLNAEHTAALRAITQEHPRSSLDEVTR
                     ELFRRAGVKVSTVTVRKALREAGIKRLKPLRRAGERATTQGSAPARYGYTAAHRRADG
                     ASGMNTDLTDAEWALVADLFERDGQRGAPARYERRHMVNACCYALRTGCAWRLLPKTF
                     PPWQASYKAFSRWAAAGVFEALHDRLRQQWRDRIGRAPEPTAAIIDAQSTRSTAQGGN
                     TGFDAGKKVKGRKRHLVVDTLGLLLAVTVTAAGVQDRDAAADVVAQACTKVPGLKKLY
                     TDAAYGGKCAQAIERTHGISVEVVRHPGNRSTGTWQDAQQPLWPEVVAKGFVVQAKRW
                     VVERTHAWNERARRLMAHHDRSNWAPLAWVWLAEARILATRLAA"
BASE COUNT          292 a          535 c          532 g          228 t
ORIGIN      
        1 accagcacca ccagcagcag cggcagcgtc aggcccagca tcagccagcg ggtgcggcgc
       61 gacagccggc tttcgcctgc gggcgaggtc aagtcagagc ctgtgaatca atccggcgcg
      121 cccgagaggg tggcccagga ggcgccaatc taggcgcaaa gcacagccgt agctcgggct
      181 acggcgagca tttgcaacga cgagtggcgt cttctgggac gcacgagcgg gtgtggtgga
      241 ttggttcaca ggctctcaga gggtgtagac gaaaacaata taccgcgcca tactcttgcc
      301 tagacaagat gcaggagagc gcggatggcg accaagatgc aaggcgagca acccggccgc
      361 cccgggcgca agccctcgct gaacgctgaa cacactgcgg cgctgcgtgc gattacacag
      421 gagcatccgc gctcttcgct ggatgaagtg acgcgtgagt tgtttcgacg tgccggtgtc
      481 aaggtcagca ccgtcactgt gcgcaaggcg ctgcgcgagg ccggtatcaa gcggctcaag
      541 ccactgcgcc gtgccggcga gcgcgcaaca actcaaggct ctgcgcctgc gcgatacggc
      601 tacacggctg ctcatcgccg cgccgatggc gccagcggca tgaacaccga tctgaccgac
      661 gccgaatggg cgctggtggc ggacctgttc gagcgcgacg ggcagcgcgg agcgcccgcc
      721 cgttatgagc gccgccacat ggtcaacgcc tgctgctacg cgctgcgcac cggttgcgca
      781 tggcggctgc tgcccaagac gttcccgcct tggcaggcga gctacaaagc cttcagccgc
      841 tgggctgcgg ccggagtgtt cgaggccctg cacgaccgcc tgcgccagca atggcgcgac
      901 cgcatcggcc gggcacccga gccgacagcc gcgatcatcg acgcgcagag tacgcgcagc
      961 actgcgcagg gcggcaacac cggattcgac gcaggcaaaa aggtcaaagg ccgcaagcgc
     1021 catctggtgg tggacacgtt gggcttgctg ctggccgtca cggtcaccgc agccggcgtg
     1081 caggaccgtg acgctgcggc ggacgttgtg gcgcaggctt gcaccaaagt ccccggtctg
     1141 aagaagctgt acaccgacgc cgcctacggc ggcaagtgcg cccaggccat tgagcgaacg
     1201 cacggcatca gcgtcgaggt cgtccgccac cccggcaacc gcagcacggg cacttggcaa
     1261 gacgcccagc agcctctgtg gcccgaagtc gtggccaagg gcttcgtcgt gcaggccaag
     1321 cgctgggttg tcgagcgaac tcatgcctgg aacgagcgcg cacgccgcct catggcacac
     1381 catgaccggt ccaactgggc ccccctcgca tgggtctggc tcgccgaagc gcgtatcctc
     1441 gcaaccagac tggcagcgta gatcatttcg tctacaccct ctgagggggc ctcaagtctc
     1501 gcgcgccgcc gccgaaacca gtggatgcgt ctcgcgtgcg cacttgcgct ggccttgctg
     1561 ctgcccgacg tcgcggccgc ggccggc
//
LOCUS       DASWIY010000385         9302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3567883, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000385 DASWIY010000000
VERSION     DASWIY010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9302
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3567883"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VGA59_13715"
     CDS             complement(<1..1206)
                     /locus_tag="VGA59_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEX9720778.1"
                     /translation="MTTAAEISHLQDHGLYSAANEHDACGVGFVAHIKGEKSHAIVQQ
                     GLKILENLDHRGAVGADKLMGDGAGILIQLPDALYREEMWRQGVELPPPGEYGVGMIF
                     LPKEHASRLACEQELERAIKTEGQVLLGWRDVPVDRDMPMSPAVRKKEPIIRQLFIGR
                     GNDVIVQDALERKLYVIRKTASAAIQNLHLKHSKEYYVPSMSSRTVVYKGLLLADQVG
                     TYYLDLKDPRCVSALGLVHQRFSTNTFPEWPLAHPYRYVAHNGEINTVKGNYNWMKAR
                     EGVMSSPVLGADLKKLYPISFANQSDTATFDNCLELLTMAGYPISQAVMMMIPEPWEQ
                     HTTMDERRRAFYEYHAAMLEPWDGPASIVFTDGRQIGATLDRNGLRPSRYCVTDDDLV
                     IMASESGVLP"
     gene            complement(1396..2091)
                     /locus_tag="VGA59_13720"
     CDS             complement(1396..2091)
                     /locus_tag="VGA59_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9720779.1"
                     /translation="MARLPRLTIPGYPHHIIQRGNNRQPIFAANADYEYLLSLLDENA
                     RQAGVAIHAYVLMSNHFHVLATPETAEGIPQMMQAIGRRYVRYFNQRQGRTGTLWEGR
                     YKSTLIQSERYLLACMVYIDLNPVRAALVSDPGDYPWSSHGHYVGRRHDRLITPHALY
                     WALGNTPFARDAAYGELVRSGITAQQQRALTESALRGWALGEASYVAELQRRTARRVS
                     KTHAGRPPSAPAR"
     gene            complement(2263..3099)
                     /locus_tag="VGA59_13725"
     CDS             complement(2263..3099)
                     /locus_tag="VGA59_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9720780.1"
                     /translation="MKQDALELRPGQSVELLKELHILTREGRMNQDSRRKLKQVYHLY
                     QFIEKLLLELPAGPDRPTLADHGAGKSYLGFILYDLFFKERGTGHIFGVETRPELVEK
                     SRELAARLGFERMSFLNLSAAQAADAPVLPPQIDMVTALHACDTATDDAIAFGLRKRA
                     RFLVLVPCCQAEVAGCLRQNKALALSRTPLAELWRHPLHTRELGSQLTNVLRCLYLEA
                     CGYQVTVTELVGWEHSLKNELILASFNGQKKRGAAMRLRALLAEFGLQGLEAIRYPGL
                     DA"
     gene            complement(3096..3650)
                     /locus_tag="VGA59_13730"
     CDS             complement(3096..3650)
                     /locus_tag="VGA59_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEX9720781.1"
                     /translation="MKHAPDVVVRDTRNWLERAVIGLNLCPFAKTVHVKGRVHFAVSG
                     ATHAAQLVEDLAGELDALVGTDAAARETTLLMAPECLADFLEFNDFLPRAHRLIRKRG
                     LEGVIQLASFHPAYQFAGTEPDDVANCTNRSPYPTLHLLREASIEWAVQAFPQAQAIY
                     EANIETLRRLGTAGWTALDVGPSR"
     gene            complement(3745..4986)
                     /locus_tag="VGA59_13735"
     CDS             complement(3745..4986)
                     /locus_tag="VGA59_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9720782.1"
                     /translation="MTARRLDPALVVVLGGISAALHVGKLPPALPVLGEALGVTLLQA
                     GFLLSLVQLAGMTLGLPVGLAADSLGLKRTMVGGLAILSLASLLGGWARGPETLMLLR
                     GVEGLGFLLAAMPAPSLIRRLVDPARMNTTLGLWGAYMPFGTAVALLCGPLFMVWAGW
                     QGWWWLLAAASSLMALWLWVALPPDGNHSHLRGSGGAQGWFERLRRTLSSAGPWLVAL
                     SFAVYSSQWLAVIGFLPSMYAQAGLSAAVAGPATALAAAVNMGGNIASGRLLQQGLRP
                     ERLLYLGFLTMGLGGFFAFAPILDAIDARLAAGLRYGCVLMFSLVGGLIPGTLFSLAV
                     RLAPGERTISTTVGWMQQWSALGQFVGPPLVAWVATRAGGWHWSWVVTGGCAAAGLGL
                     AAFIGALLRREPYSSKRETAT"
     gene            complement(4983..6113)
                     /locus_tag="VGA59_13740"
     CDS             complement(4983..6113)
                     /locus_tag="VGA59_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX9720783.1"
                     /translation="MTLARYACDPARSRGRRHAEEGAPSRTEYQRDRDRVVHSTAFRR
                     LVYKTQVFLNHEGDLFRTRLTHSLEVAQLGRSVARSLRLNEDLVEAIALAHDLGHTPF
                     GHAGQDALDDCMSGRGGFEHNLQSLRVVDELEERYPRFNGLNLTFETREGILKHCSRT
                     DAQRLEAAEPDGVARRFLDGSQPSLEAQLCNLADEIAYNAHDIDDGVRSGLITLEQLA
                     GVELFDHFRRAALGEFPELQGRRLLYESIRRMLSAQVYDVIDATRKALQTAKPAGADG
                     ARSAGRLVKFSETMQARSVVLKRFLLQNLYRHPQVAAKTGQARQVVRELFAVYLDDPG
                     QMPPDFAGRANVARTVADYIAGMTDRFAVREHERLTGRKLLA"
     gene            complement(6115..7227)
                     /gene="aroB"
                     /locus_tag="VGA59_13745"
     CDS             complement(6115..7227)
                     /gene="aroB"
                     /locus_tag="VGA59_13745"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX9720784.1"
                     /translation="MPAAHTARLHQVDIDLGDRSYPIRIGGGLLQDPATWDAVPASAQ
                     AVIVTNVTVAPLYAGQLEQALRVRHKAVHVVELPDGEEHKNWQTLNLIFDGLLRDACD
                     RKTVLYALGGGVVGDMTGFAAASYMRGVPFVQVPTTLLAQVDSSVGGKTGINHPLGKN
                     MIGAFYHPRLVVCDLDTLASLPAREFSAGLAEVIKYGPIADMAFLDWLEANIEQLMAR
                     ETAALAHAVRRSCEIKAWVVGRDEREAGLRAVLNFGHTFGHAIEAGLGYGRWLHGEAV
                     GCGMVMALKLSQRLGLVDGAFVDRVTALLRKARLPDVAPALGVDRYLELMRVDKKAEA
                     GELRFVVIDKPGSAAVRTAPEALVREVIEHSCRSAT"
     gene            complement(7238..7786)
                     /locus_tag="VGA59_13750"
     CDS             complement(7238..7786)
                     /locus_tag="VGA59_13750"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX9720785.1"
                     /translation="MSARSADPLIVLIGLPGCGKSTVGRQLARRLGLGFADSDHVIEQ
                     RLGCSIRDYFEREGEAAFRDVEESVIQELSQAGSGVLATGGGVVLRPVNRQRLRQAGH
                     AIYLRSTPEEVFRRLRHDRNRPLLQVDDPLARLRSLFEERDPLYRETAHFVIETGRPS
                     VPTLVNMILMQLELAGVIPARP"
     gene            complement(7759..9210)
                     /locus_tag="VGA59_13755"
     CDS             complement(7759..9210)
                     /locus_tag="VGA59_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720786.1"
                     /translation="MSFTMRFLSYFVTLLVAVLFTACGGGGGSPGITPGSQSFFTTAP
                     ADLTLALGSAQTFTVAGGKAPYSAASNNSAVAVSAITDSTLTLGAVSPGVALVTLRDA
                     SGATLALSVTVRPDRALFTTAPASIALAVGLSSAQTYTVGGGLGPYSVASSNPGAISA
                     VLNGTNLTITGLAVGIGNVQLMDSVGSTVVIQVAVAATPAVALFTTAPSAVTLAPGAA
                     NAQTFSIGGGNPPYVATSSTIGIATVTLLTANTFNITGVAAGPANIVVRDGSGATTSI
                     AVTVAGAAPLVLNPVTVSAFIGDTVYSTISGGVAPFTVLNGFPDVADVDIGTVSAGGL
                     FTANSSGNVLRIRVKQAVATGVIVVKDSAGGSANFTLTATPGTNVISLAPSSLTIGEE
                     FAGPIQLILRGAVGTTNIFSSNPDLIAVTTPVAASASGTIVTVTKTVGEVCTTGAVTI
                     TAIDANAAKAVALITVEDHGNNPDPCPPVVPTP"
BASE COUNT         1670 a         3066 c         2984 g         1582 t
ORIGIN      
        1 cggcagcacg ccggattccg aggccatgat caccaggtca tcgtcggtaa cgcaatagcg
       61 cgagggccgc aggccgttgc ggtccagggt ggcaccgatc tggcggccgt cggtgaagac
      121 gatggaggcc ggtccgtccc agggttcgag catggcggcg tggtattcgt aaaacgccct
      181 gcgccgctcg tccatggtgg tgtgctgctc ccacggctcc gggatcatca tcatcacggc
      241 ctggctgatc gggtagccgg ccatggtcag cagttcgagg cagttgtcga aggtggccgt
      301 gtcggactgg ttggcaaagc tgatcggata gagcttcttg aggtccgctc ccagcacggg
      361 tgaggacatc acgccttcgc gcgccttcat ccagttgtag ttgcccttga cggtgttgat
      421 ctcaccgttg tgggcgacgt agcgataagg gtgggccagc ggccactcgg ggaaggtgtt
      481 ggtggaaaag cgctggtgca cgaggccgag cgccgaaaca cagcgcggat ccttcaggtc
      541 caggtaatag gtgccgacct ggtcggccag cagcaggccc ttgtagacga cggtacggct
      601 cgacatgctg ggaacgtaat attccttgct gtgcttcaga tgcaggttct ggatggcagc
      661 gctggccgtc tttcgaatca cgtacagctt gcgttccagg gcgtcctgca cgatcacgtc
      721 gttgccgcgg ccgatgaaca gctggcggat gatcggctcc ttcttgcgta cggccggcga
      781 catcggcatg tccctgtcca ccggtacgtc gcgccagccc aacaggacct ggccctcggt
      841 cttgatcgcg cgttcgagct cctgctcgca agccagccgc gacgcgtgct ccttgggcag
      901 gaatatcatg ccaaccccgt attccccggg cggcggcagt tcgaccccct gcctccacat
      961 ttcttcgcga tacagcgcgt cgggcaactg gatcagaatg cccgcgccgt cgcccatcag
     1021 cttgtcggcg cccaccgcgc cccggtggtc caggttctcc aggatcttga gaccttgctg
     1081 gacgatggcg tggcttttct cgcccttgat gtgcgcgaca aagcccaccc cacaggcgtc
     1141 atgctcgtta gcggccgaat agaggccatg gtcctgcaaa tgggaaattt cggcagccgt
     1201 ggtcatggcg cgctcctgaa cgttaggcgg aaactccaag aatactgcgc tgcaacaacg
     1261 ttgccaagca aaataattgg ggtcagattc caattatctt ctgaagattc agctggaaat
     1321 ttaattaggg acagattaaa tatgctcgac agccgcactc gtttttggcc cggcgccata
     1381 gcccgagaag acgactcaac gcgcgggcgc gctcggtggg cgccccgcgt gggtcttgct
     1441 cacccgccgg gccgtgcgtc tttgcagttc ggccacatag ctggcctcac ccaaggccca
     1501 gcctctgagg gccgactcgg tcagggcccg ctgctgctgt gcagtgattc cggaacgcac
     1561 cagttcccca tacgccgcgt cccgggcgaa cggcgtgttt cccagcgccc agtaaagcgc
     1621 gtgcggtgtg atcagccggt cgtggcggcg cccgacatag tgcccgtggc tcgaccaggg
     1681 atagtcgccg ggatccgaca ccagtgcggc gcgcaccggg ttgaggtcga tgtacaccat
     1741 gcaggccagc aaatatcgtt cgctctggat caatgtggac ttgtatcgtc cttcccacaa
     1801 tgtgccggtg cgcccctgcc gctggttgaa atatcggaca tagcgacgcc cgatcgcctg
     1861 catcatctgg ggaatgccct cagcggtttc gggagtggcc aacacgtgga aatggttgct
     1921 catcagcaca taggcatgga tggcgactcc agcctgccgt gcattctcgt cgagcagcga
     1981 tagcaaatac tcgtagtcgg cattggcggc gaatatcggc tgccggttgt tgccccgctg
     2041 gatgatgtga tgcggataac cgggaatagt caagcgcgga agacgagcca tggcgaggtt
     2101 tggttatcgg agtcgatcat aaattggaat ctgaccccaa tttcccaatt tcccaatttc
     2161 cgcccagcag attcggccgt agacagaagc cggaaccccg atctcacggc aagtacttct
     2221 cgcacaaggg aaaactcacc ccggggctcc aggaacttcg ggctaagcgt ccagtccggg
     2281 atagcggatg gcttccaggc cctgcaagcc gaactccgcc agcagggcgc gcaagcgcat
     2341 cgccgcgccg cgttttttct gcccattgaa actggccagg atgagttcgt tcttcaggct
     2401 gtgctcccac cctaccagtt ccgtcaccgt gacctggtag ccacaggcct ccagatagag
     2461 gcaacgcagc acgttcgtca attggctgcc cagttcgcgc gtatgcaatg gatggcgcca
     2521 cagctcggcc agcggggtgc gcgagagggc cagcgccttg ttctgccgca ggcagcccgc
     2581 aacctccgcc tggcagcacg gcaccaacac caaaaagcga gcgcgcttgc gcaagccgaa
     2641 ggcgatggcg tcgtcggtcg cggtgtcgca cgcatgaagc gccgtcacca tgtcgatctg
     2701 cggcggcagc acgggggcat cggcggcctg cgccgcgctc aggttgagaa aggacatgcg
     2761 ctcgaacccc agccgcgcgg cgagttctcg cgacttttcc accagttcgg gccgggtttc
     2821 gacgccgaag atgtgacccg ttccccgctc cttgaagaac aggtcgtaaa gaatgaagcc
     2881 gaggtacgac ttcccggcgc catggtcggc cagagtcggc cggtccggtc cggccggcag
     2941 ctccagcagc agcttttcga tgaactggta aaggtgatag acctgcttga gcttgcgccg
     3001 cgagtcctgg ttcatccggc cctcgcgggt gaggatatgc aattccttga gcagctcgac
     3061 cgattggcct ggccgcaatt cgagcgcgtc ctgtttcacc gggaagggcc cacgtccagc
     3121 gcggtccagc ccgctgtccc cagccgccgc agggtctcga tattggcttc gtagatggcc
     3181 tgggcctgcg gaaacgcctg aaccgcccat tcgatgcttg cttcgcgcag caggtgcagc
     3241 gtgggatagg gcgaccggtt cgtgcagttc gccacgtcgt caggctcggt ccccgcgaac
     3301 tggtaggccg ggtggaaact cgccagctgg atcacgcctt ccaggccccg cttgcggatc
     3361 agtctatgag cccgcggcag gaaatcgttg aattcgagaa agtccgcgag acactccgga
     3421 gccatcagca gcgtggtctc ccgcgccgcg gcatccgtgc cgaccagcgc gtcgagctcg
     3481 ccggccaagt cctcgaccag ctgcgcggcg tgggtggcac cgctgaccgc gaagtgaacc
     3541 ctgcccttga cgtgaacggt tttggcaaac ggacacaggt tcaatccgat gaccgctcgc
     3601 tccagccaat tgcgcgtgtc gcgcacgacc acgtcgggag catgtttcat ggctcgtcaa
     3661 accggcaagg ctcgcaggca acctgcgcat tcagttgatt caagaggctc acttcggcgg
     3721 acccggcgtt ggatcgaaac gattctacgt ggcggtttcg cgctttgacg aatagggctc
     3781 gcggcgcagc agcgccccga tgaacgcagc cagcccaaga cccgctgcag cacagccgcc
     3841 cgtcaccacc cagctccaat gccagccgcc ggcacgcgtg gcaacccagg ccaccagcgg
     3901 cggtcccacg aactggccca gcgcggacca ctgctgcatc cagcccaccg tggtggagat
     3961 cgtgcgttcc cctggcgcca accgcaccgc gagcgagaac aaggtaccgg gaatgaggcc
     4021 gccgaccagg gagaacatca gcacgcagcc gtagcgcagc ccggccgcga gccgcgcgtc
     4081 gatcgcgtcc aggatcggcg cgaacgcgaa aaacccgcct aggcccatcg tcaggaagcc
     4141 caggtagagc aggcgttccg gacgcaggcc ttgctgcaac agtcgccccg aagcgatgtt
     4201 gccgcccatg ttcaccgctg cggcaagcgc cgtggcggga cccgccacgg ctgcgctgag
     4261 cccggcctgg gcgtacatcg agggcaggaa tccgatcacc gccagccatt ggctcgagta
     4321 cacggcgaag ctcagcgcca cgagccacgg cccagccgac gacagggtcc ggcgcaaccg
     4381 ctcgaaccag ccctgggcgc cgccgctgcc gcgcaggtgc gaatgattgc cgtccggggg
     4441 caacgccacc cagagccata gcgccatcaa cgatgaggcc gccgcgagca gccaccacca
     4501 accctgccag cccgcccaca ccatgaacag cggaccgcac aacagcgcga cggcggtgcc
     4561 gaacggcatg tacgcgcccc acagtccgag ggtggtgttc atgcgtgccg gatcgaccag
     4621 ccgccgaatc aggctgggcg ccggcatggc cgccagcaga aagcccaggc cctcgacgcc
     4681 gcgcaagagc atcagcgtct cgggcccgcg agcccagcct ccgagcaggc tcgccaacga
     4741 aaggatggcg agcccgccga ccatcgtgcg tttcaggccc aggctgtccg cggccaagcc
     4801 gaccggcagg cccagggtca tgcccgccag ttggaccagc gacagcagaa agcccgcctg
     4861 cagcagcgtc acgccgagcg cctcgcccag caccggcagg gccggcggca acttgccgac
     4921 atgcagtgcg gcgctgatgc cacccagcac caccactaac gcgggatcca gtcgccgcgc
     4981 cgtcacgcca gcagtttccg gccggtgagg cgctcgtgtt cgcgcaccgc gaagcggtcg
     5041 gtcatgcctg cgatgtagtc cgccacggtc ctggcgacat tggcccggcc cgcgaagtcc
     5101 ggcggcattt gccccggatc gtccagatag accgcgaaca gttcgcgcac cacttgcctg
     5161 gcctgcccgg tcttcgcggc gacctgggga tgacggtaca ggttctgcag caagaagcgc
     5221 ttcagcacaa cggatctcgc ttgcatcgtc tcgctgaatt tcaccagccg gcctgccgag
     5281 cgcgccccgt ccgcccctgc gggcttggcc gtttgaagcg ccttgcgggt tgcgtcgatc
     5341 acgtcgtaca cctgggcact gagcatgcgg cgaatggatt cgtacaagag acggcggccc
     5401 tgcagctccg ggaattcgcc cagcgccgcc cgccggaaat ggtcgaacaa ctcgactccg
     5461 gccagctgct ccagcgtgat cagccccgag cgcacgccgt cgtcgatgtc gtgggcgttg
     5521 tacgcgatct cgtcggcaag attgcacagc tgggcctcca ggctgggctg gctcccatcc
     5581 aggaaccggc gcgcgacgcc atcgggctcg gccgcctcca ggcgttgcgc atcggtgcgc
     5641 gagcagtgct tgaggatgcc ctcgcgcgtc tcgaaagtga ggttcaggcc gttgaagcgc
     5701 ggatagcgct cctcgagctc gtccaccacg cgcaggctct gcaggttgtg ctcgaagccg
     5761 ccccgcccgc tcatgcaatc gtccagcgcg tcctggccgg catggccgaa cggcgtgtgg
     5821 cccaggtcat gcgctaacgc gatcgcttcg accagatcct cgttgagccg caaagacctg
     5881 gcgacggagc ggcccagttg cgccacctcg agcgagtgtg tgagccgcgt gcggaacagg
     5941 tcgccctcgt ggttcaggaa aacctgggtc ttgtagacca ggcgccggaa ggcggtggag
     6001 tgaacgacgc ggtcccggtc gcgctggtac tcggtccggc tgggcgcgcc ttcctcggcg
     6061 tggcgccggc cgcgcgaccg ggccggatcg caggcgtagc gggccagggt catttcaggt
     6121 ggcgctgcgg caactgtgtt cgatgacttc acgcaccagc gcctcgggcg cggttcgcac
     6181 tgccgcgctt ccaggcttgt cgatcacgac aaatctgagt tcgcccgcct cggccttctt
     6241 gtccacccgc atcagctcca ggtagcggtc cacaccgagc gcgggtgcca cgtcgggcag
     6301 ccgggctttg cgcagcaacg cggtgacgcg atcgacgaaa gccccatcga ccaggcccag
     6361 gcgctgcgac agcttcagcg ccatcaccat gccgcatccg acggcttcac cgtggagcca
     6421 tcggccatac ccgaggccag cctcgatcgc atgaccgaac gtgtggccga aattgaggac
     6481 ggcgcgcagg ccggcttcgc gctcgtcccg gccgacgacc caggccttga tctcgcagct
     6541 gcgcctgacc gcatgcgcca gggccgcggt ctcgcgggcc atcagctgtt cgatattcgc
     6601 ttccagccaa tccaggaacg ccatgtcggc gatgggtccg tatttgatga cttcggccag
     6661 gcccgcgctg aattcgcgcg ctggcagact cgctaacgta tccaggtcac agacaacaag
     6721 gcgcgggtgg tagaacgcgc cgatcatgtt cttgccaagc ggatggttga tgccggtctt
     6781 gcctcccacc gatgaatcca cctgggccag caacgtcgtc ggcacctgga cgaagggcac
     6841 gccgcgcatg tagctggcag ccgcgaagcc ggtcatgtcg cccaccacgc cgccgcccag
     6901 cgcgtacagg acggtcttgc gatcgcaggc gtctcgcagc aagccatcga aaatcaggtt
     6961 cagggtctgc cagttcttgt gctcttcgcc gtcgggcagc tcaaccacat gaactgcttt
     7021 gtgacgcacc cgcaaggcct gctccagctg cccggcatac agcggcgcca ccgtcacgtt
     7081 cgtgacgatg acggcctggg ccgatgccgg caccgcgtcc caggtggccg ggtcctgcag
     7141 caagccgccg ccgatgcgga tcgggtagct gcggtcaccg agatcgatgt cgacctggtg
     7201 caggcgtgcg gtgtgagcgg ctggcatagc cgcaagttta agggcgggcg ggaatgaccc
     7261 cggccagttc cagttgcatc aggatcatgt tcacgagcgt cggcaccgag ggacggccgg
     7321 tctcgatgac gaaatgggcc gtctcgcgat agagggggtc gcgctcctcg aagaggctgc
     7381 gcagccgggc caaggggtcg tcgacctgca gcagcggccg gttgcggtcg tgccgcaggc
     7441 gcctgaacac ttcctccggc gtcgagcgca ggtaaatggc gtggccggcc tggcgcagcc
     7501 gttggcgatt gaccggcctg agcacaacgc ctcccccggt tgccaacacg ccggaaccgg
     7561 cctggctcag ttcctggatc accgactcct cgacgtcgcg aaacgccgct tcaccttcac
     7621 gctcgaaata gtcgcggatc gagcagccca gccgctgctc gatcacgtgg tccgaatcgg
     7681 cgaagccgag tcccagccgc cgggcgagct gccgtccgac agtggacttc ccgcagccgg
     7741 gcaggccgat gaggacgatc agggggtcgg cactacgggc ggacacggat cggggttgtt
     7801 cccatggtcc tcgacggtga tcagcgccac agccttggcc gcgttggcat cgatcgcggt
     7861 gatggtcacc gcccctgtcg tgcacacctc accgaccgtc ttggtgaccg tcacgatcgt
     7921 tccagaggcg ctcgcggcga ccggcgtggt gacggcgatc aaatccgggt tgctcgagaa
     7981 gatgttggtc gtgccgacag cgccacgcag gataagctgg atcggtccgg caaattcctc
     8041 gccgattgtc aggcttgagg gcgcgagcga tatcacattg gtgccgggcg tcgccgtgag
     8101 cgtgaaattg gccgaaccgc cggcggaatc cttgacgaca atgacgccgg tggcaacggc
     8161 ctgcttcacc ctgattcgca agacattccc gctcgagttt gccgtgaaca atcccccggc
     8221 gctcacggtg ccgatgtcca cgtccgcaac atcggggaag ccgttcaaga cggtaaacgg
     8281 cgccacgcct ccgctgatgg tcgagtacac ggtatcaccg atgaaggcgc tgacagtgac
     8341 cgggttcagc accaatggcg ccgccccggc gaccgtcacg gcgatcgagg ttgtcgctcc
     8401 gctgccgtcc cgtacaacga tgttggcggg gcccgccgcc actccggtga tgttgaacgt
     8461 attcgcggtg agcagtgtta ccgtggcgat tccgatggtg ctgctggtgg cgacatacgg
     8521 cgggttcccg ccaccaattg aaaaagtctg cgcattcgcg gctccgggcg ccagggtgac
     8581 tgctgaaggg gcggtggtga acaacgccac ggctggggtc gctgccaccg cgacctggat
     8641 gaccacggta gagccaacgc tgtccatgag ctgaacgttg ccgatcccaa ccgccagccc
     8701 ggtgatggtc aggttggtcc cattgagtac ggccgagatc gcaccgggat tgctgctggc
     8761 aaccgagtac ggccccaggc cgccgccaac cgtgtaagtc tgggcggaac tcaatccgac
     8821 agccagtgcg atgctcgccg gcgcggtcgt gaacagtgcc cggtctggcc tgacggtaac
     8881 cgacagtgcc aaggtagcgc cgctcgcgtc ccgcagcgtg accaaggcga cgcccggact
     8941 taccgcgccc agcgtcaagg tcgagtcggt gatcgcggag accgcaaccg cactgttatt
     9001 gcttgctgcg gagtagggag cttttcctcc cgccaccgtg aaggtctggg cgctgcccag
     9061 tgcgagcgtc agatcggccg gcgcagtcgt gaagaaggac tggctgcccg gggtgattcc
     9121 gggagaacca cctccgcccc cgcaagcagt gaaaaggacg gcaactagca atgtcacgaa
     9181 ataggacaga aaacgcatgg tgaagctcat ggttagtaat ggttatagat ttgttgaatc
     9241 accgtgccgc ggcacggtcg gcgatcatct tgggcgtgat gaacaccagc atttcctgct
     9301 tg
//
LOCUS       DASWIY010000386         3484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_635728, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000386 DASWIY010000000
VERSION     DASWIY010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635728"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..465
                     /locus_tag="VGA59_13760"
     CDS             211..465
                     /locus_tag="VGA59_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX9720787.1"
                     /translation="MKITFKLFATLTDYLPPGARRSNVLELEVPGDASISQIIEPFGL
                     PPKLVHLVLVNGTFVQPADRLRRTLKEGDVLAIWPPIAGG"
     gene            478..777
                     /locus_tag="VGA59_13765"
     CDS             478..777
                     /locus_tag="VGA59_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720788.1"
                     /translation="MQSAYAERFEREMGCTEAEWLMWLPAAVGDREWRQQAGKATVSI
                     SPGILRLAWYTGEPRVIAQVRLPRLHVSFRFEGLDAGQRLAFMKRFDLYMQRGGG"
     gene            complement(815..2116)
                     /locus_tag="VGA59_13770"
     CDS             complement(815..2116)
                     /locus_tag="VGA59_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9720789.1"
                     /translation="MSEIAVTVLLVVTLFLILGSGVWIGLTLSGVAWIGMQLFSSRPA
                     GDAMAVTIWGSSSSWTLTALPLFVWMGEILFRTRLSQDMFTGLAPWMQGLPGRLLHTN
                     VAGCTIFAAVSGSSAATCATIGKMSLPELMKRGYPEHMVVGTLAGASTLGLLIPPSII
                     MIVYGVSAEVSISRLFIAGVLPGLLLAFLFSGYIVAWSLANPARIPAADRKYSFAQKF
                     AQSRNLIPVMLLIAAVLGSIYSGFATATEAAALGVVGSLIISAVQGSMNWSAFKDSLM
                     GATRLYCMIALILAGAAFLTLSMGYIGLPRHLAEWIASLGLSQAELIAALAVFFVILG
                     CFLDGISMVVLTMGVIMPTVQKAGIDPIWFGIFVVLVVEMAQITPPVGFNLFVLQGMT
                     GRELPWIARVTFPMFILMIVAIVLIYVFPAIVTWLPRQMMN"
     gene            complement(2109..2618)
                     /locus_tag="VGA59_13775"
     CDS             complement(2109..2618)
                     /locus_tag="VGA59_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9720790.1"
                     /translation="MRRFLDAIYDSAAALAAVFLVGLLAMVLLSIASRQLHFHVPGTD
                     AYAGYLMAASGFLALAHTLKHGEHIRVTLLVNALRGRWKRAFEVWALVAATLLALLFA
                     FYASRLSWQSYVFHDISTGNDATPLWLPQSAMALGTIILALAFIDELVLEVRGRQAAS
                     TAAGALRNE"
     gene            complement(2623..>3484)
                     /locus_tag="VGA59_13780"
     CDS             complement(2623..>3484)
                     /locus_tag="VGA59_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9720791.1"
                     /translation="QTENVQQFANDVDKATGGKLKITVHPNASLFKAPEIKRAVQGGQ
                     AQAGEVLLVNFQNEWPIYGADGLPFLADSYDSALKLYKAQRPMLEKKLAGDGIMLLYT
                     VAWPPQGIYSKKPLASAADLKGIKWRAYSPATSRIAELVGAQPVTVQAAEVSQAFATG
                     VAESMMSSGSTGWDSKLYEHVKYWYDTQAWLPKNAVLVNKKAFDALDKPTQDAVLKAG
                     AAAEERGWAASKRANLETLEKLKANGMQILPPSPQLKADMKKVGDTMLKEWLDKAGPE
                     GKQLVDAYRK"
BASE COUNT          647 a         1105 c         1131 g          601 t
ORIGIN      
        1 tcgctgaact aagaggctgt catcccgggc cttcaggttg tggctgtcat cccgggctcg
       61 accccggatc aggtccgggg caggctccgg gatccatgac ttcatggatt gcggatcaag
      121 tccgcaatga caagtcaata aggctgtcat cacgggctcg accccggacc tgattcgggg
      181 tccgtaatat ccatccccgc atttccgccg atgaagatca ccttcaagct attcgccaca
      241 ctcaccgact acctgccgcc cggtgcacgg cgcagcaacg tgctggaact ggaggtgccg
      301 ggcgacgcca gcatcagcca gatcatcgag ccgttcggcc tgccgcccaa gctggtgcac
      361 ctcgtgttgg tgaacggcac tttcgtgcag cccgccgatc gcttgcgccg tacgctgaag
      421 gaaggcgacg tgctcgcaat ctggccgccg atcgcgggcg gctagggcgg agccccgatg
      481 cagtcggcct acgccgagcg cttcgagcgg gagatgggct gcaccgaggc cgaatggctg
      541 atgtggttgc cggcagccgt tggcgaccgc gagtggcggc agcaagccgg caaggccact
      601 gtctcgatct ctccaggaat ccttaggctg gcttggtaca ccggcgagcc gcgcgtgatc
      661 gcccaggtgc gattgccgcg gttgcatgtg agtttccgct tcgaagggct ggacgccggc
      721 cagcgcttgg cgttcatgaa gcggttcgac ctgtacatgc agcgcggcgg cgggtgaggg
      781 tggcccgtgg acccgcacgt cattgcgacc ggcgctagtt catcatttgc cgcggcagcc
      841 aggtcacgat ggcgggaaag acatagatca ggacaatcgc cacgatcatc agaatgaaca
      901 tcgggaaggt gactctggcg atccacggca gctcacgccc ggtcatgccc tgcagcacga
      961 acaggttgaa gcccaccggg ggcgtgatct gtgccatttc caccaccagc acgacgaaga
     1021 tgccgaacca gatcgggtcg atgccggctt tttgcacggt gggcatgatg acgcccatcg
     1081 tcagtaccac catcgagatg ccgtccagaa agcagcccag gatgacgaag aacaccgcca
     1141 gcgcggcgat cagttccgcc tggctcagcc ccagtgaggc aatccattcg gccaggtgcc
     1201 ggggcaggcc gatgtagccc atgctcaggg tcaggaaggc ggcgcccgcg aggatcagcg
     1261 cgatcatgca gtacaggcgc gtggcgccca tcagcgaatc cttgaaggcg gaccagttca
     1321 tcgacccttg caccgccgag atgatgagcg aacccaccac gcccagcgcc gcggcctcgg
     1381 ttgcggtggc aaagccggaa tagatggagc ccagtaccgc tgcgatcagg agcatcaccg
     1441 ggatcaggtt tctggattgc gcgaacttct gcgcgaaact gtacttgcgg tcggccgctg
     1501 gaattctggc cgggttggcc agtgaccagg ccacgatgta tccggaaaac aggaacgcca
     1561 gcagcagccc cggcagcacg cccgcgatga aaaggcgcga aatggacacc tctgccgaaa
     1621 caccgtagac gatcatgatg atggacggag gaatcagcag gccgagcgtg ctggcgccgg
     1681 cgagcgtgcc caccaccatg tgctcggggt agccgcgctt cattaattcg ggcagcgaca
     1741 tcttgccgat ggtggcgcag gtggccgcgc tcgacccgga gacggcggca aagatggtgc
     1801 agccggccac gttggtgtgc agcaggcggc caggcaggcc ttgcatccat ggcgccaggc
     1861 cggtgaacat gtcctggctc agccgcgtgc gaaacaggat ttcccccatc cagacgaaca
     1921 gcggcagcgc ggtgagggtc cagctcgacg acgagcccca gatggtgacg gccatcgcgt
     1981 cgccggccgg ccgtgaggag aaaagctgca tgccgatcca ggccacgccc gagagagtga
     2041 gcccgatcca cacgccgctg cccaggatca ggaacagcgt cacgaccagc aggacggtga
     2101 cggcgatctc actcattgcg aagcgcccct gcggctgtcg aagcggcctg gcgcccgcgc
     2161 acttccagca ccagttcatc gatgaaggcc agggccagga tgatggtgcc cagcgccatc
     2221 gccgactgcg gcagccacag tggcgtggcg tcgttgccgg tggagatgtc gtgaaacacg
     2281 tacgactgcc aggacaggcg gcttgcgtaa aaagcgaaca gcagggccaa cagcgtcgcc
     2341 gcgaccagcg cccagacttc gaaggcgcgc ttccagcgtc ccctgagcgc gttgacgagc
     2401 agggtgacac gaatgtgctc gccgtgcttg agcgtgtgcg cgagcgccag gaaaccgctg
     2461 gccgccatca ggtagccggc gtaggcgtcg gttcccggaa cgtggaagtg caattgccgg
     2521 ctggcgatgg acagcagcac catcgccagc aggcccacca gaaaaactgc cgcaagagcg
     2581 gcggcgctgt cgtagattgc gtcgagaaaa cggcgcatcg gcctacttgc ggtaggcgtc
     2641 caccagctgc ttgccctcgg ggccggcctt gtccagccat tccttgagca tcgtgtcgcc
     2701 caccttcttc atgtcggcct tgagctgggg cgaaggaggc aggatctgca tgccattggc
     2761 cttgagcttt tccagcgtct cgaggttcgc gcgcttgctg gcggcccagc ctcgttcttc
     2821 ggccgcggct ccggccttca gcacggcgtc ctgggtgggc ttgtccagcg cgtcgaaagc
     2881 cttcttgttg acgagcaccg cgttcttggg cagccacgct tgcgtgtcgt accagtactt
     2941 cacatgttca tagagcttgc tgtcccagcc ggtcgagccc gaactcatca tgctttccgc
     3001 gacaccggtg gcaaacgcct ggctgacctc cgccgcctgc accgtcacgg gctgtgcgcc
     3061 gaccaattcc gcaatgcgtg aagtggccgg actgtaggcg cgccacttga tgcctttgag
     3121 gtccgcggcg gacgccagcg gcttcttgga gtagatgccc tggggcggcc aggcaaccgt
     3181 gtagagcagc atgatgccgt ccccggcgag tttcttttcc agcatcggcc tctgggcctt
     3241 gtacagtttc agggccgagt catagctatc ggccaggaag ggcaggccat cggccccgta
     3301 gatcggccat tcgttctgga aattcaccag caggacctcg ccggcctgag cttggccgcc
     3361 ctgcaccgcc cgcttgattt caggcgcctt gaacagggaa gcattgggat gcaccgtgat
     3421 cttcagcttg ccgccggtgg ccttgtccac gtcattggcg aactgctgca cgttttcggt
     3481 ctgg
//
LOCUS       DASWIY010000387         6851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2496295, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000387 DASWIY010000000
VERSION     DASWIY010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496295"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VGA59_13785"
     CDS             complement(<1..432)
                     /locus_tag="VGA59_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9720792.1"
                     /translation="MVVPLVSMATMKNVHGNTLDHLGEAIVAGRYRPGASIPPEPRLG
                     EALGVSRTVIREAVKSLVGKGLVSTGPKVGTRVLPEEHWNWFDPDVILWQAKAGLTPE
                     FLRDLQDLRRVVEPAAVRMAAERATVQDIAEIEEAFAGMCKA"
     gene            541..1614
                     /gene="ugpC"
                     /locus_tag="VGA59_13790"
     CDS             541..1614
                     /gene="ugpC"
                     /locus_tag="VGA59_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX9720793.1"
                     /translation="MAEVQIKGVEKYFGATHIIRGVDIDMEDGQFTVLVGPSGCGKST
                     LLRMIAGLEEITSGEILIGGRVVNNMMPKERDIAMVFQNYALYPHMTVRDNMAFSLML
                     ARQPKAVADERVRKAADILGLNELLDRYPRQLSGGQRQRVAMGRCIVRDPQVFLFDEP
                     LSNLDAKLRVQMRTEIRELHQRLKTTSVYVTHDQIEAMTMADKIVVMRDGIVEQTGNP
                     LDLYDHPANQFVAGFIGSPAMNFLPGTLRRSNAGMSVELNGGARLAAPPGTPDADGQS
                     VVFGTRPEHLTLSDNGGIPARVVVMEPSGMDTFIVCRHEEMQLSAVFRERHDFAPGST
                     IRLQPDLQRAHLFDAGTGRRLAA"
     gene            1670..2998
                     /locus_tag="VGA59_13795"
     CDS             1670..2998
                     /locus_tag="VGA59_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9720794.1"
                     /translation="MTDFNRRKFLEGSAGVAAAATLGAGAIFAPAVHAQAWSNQPEKG
                     AKLRVLRWSRFVQGDIDQYMKNVAAFTAKTGIEVRVDNEGWEDVRPKAAVAANTGAGP
                     DIILSTHDDANLYPEKLVDVTDLANYLGRKYGGWYPVGEAYLRPNGKNWIGVPLGCAG
                     NTLVYRESQVKAAGFDKFPRDTDGFLRLMKALKEKGTPGGMALGNATGDGNVWTHWLL
                     WAFGGQLVDKNNKVVVNSPQTVKALEYARELYQTFVPGTLSWLDPNNNKAFLDGQVSV
                     TPNGISIYYAAKTSKDAKLQEMAKDINHVNMPIGPAGVPTELNLFFNQMIFKHSKYPK
                     AAKEFIRFMMEEEQYGPWMQAAIGYVTQPLVAYEKNPIWTVDPKHTPYRDSMKIMRPI
                     GYAGKLGYASAGAAADFIVVNMVAEAGSGSKTPQEAAERAQKRAERYYKV"
     gene            3007..4014
                     /locus_tag="VGA59_13800"
     CDS             3007..4014
                     /locus_tag="VGA59_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9720795.1"
                     /translation="MPADPTQRPRETGGPTAALGASAGSAASPRDRRPSTLERLQNSR
                     NALGFIFLFPAALLLVLFLTYPLGLGVWLGFTDTKVGRPGVWVGLENYEFIAQDAVTQ
                     LALFNTLFYTFVASVLKFFLGLWLAVLLNRNIRLKTFFRAVILLPYIVPTALSAIAFW
                     WIYDAQFSVISWALFKLGLIDRYIDFLGEPWNARLSVIAANVWRGVPFVAITLLAGLQ
                     TISPSYYEASAIDGATPWQQFRHVTLPLLTPIIAVVMTFSVLFTFTDFQLIYVITRGG
                     PLNATHLMATLSFQRAIPGGALGEGAAISIAMVPFLLAAIMFSYFGLQRRAWQQGGSD
                     K"
     gene            4011..4886
                     /locus_tag="VGA59_13805"
     CDS             4011..4886
                     /locus_tag="VGA59_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9720796.1"
                     /translation="MKADDTIDSAGMTYLETPLRKAVVVYLPLAVFVFVLLFPFYWMG
                     ITAFKPNNELLSREGNPFWVIAPTLAHFKKLLFDTSYPQWMWNTVLVSVVATFFSLAA
                     AVFAAYAIERLRFSGAKQVGLAIFLAYLVPPSILFIPLASVVFQLGLFDTRLALILTY
                     PTFLIPFCTWLLMGYFRSIPYELEECALIDGASRWQILTKIVLPLAVPGLISAGIFAF
                     TLSWNEFIYALTFVSSSEIKTIPVGVVTELVEGDVYHWGSLMAGALLGSLPVAFIYSF
                     FVEHYVSGLTGSVKE"
     gene            4981..6723
                     /locus_tag="VGA59_13810"
     CDS             4981..6723
                     /locus_tag="VGA59_13810"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX9720797.1"
                     /translation="MSNSHPPRKKPEELRSQQWFGRQDRDGFNYRSWVKGKGVPHDQF
                     DGRPVIGICNTFSELTPCNSHFRTLAEQVKIGVYEAGGFPLEFPVMSLGETLLRPTAM
                     LYRNLASMDVEESIRGNPIDGVVLLMGCDKTTPSLVMGASSVDIPTIGVSGGPMLNGK
                     WRGQELGSGTGVWSMSEQVRAGTLKLQDFFEAESCMHRSHGHCMTMGTASTMACMVEA
                     LGIGLPGNAAYPAVDGRRNVLARMAGRRIVEMVHEDLKLSKILTREAFENAIKTLAAI
                     GGSTNAVIHLIAIARRIGVELAIEDFDRLASDLPCLVNLQPSGEHLMEDFCYAGGLPV
                     VMKEIARHLHLDAITANGRKVGENIADAVNYSTEVIQPLARPFKEKAGIAVLRGNLAP
                     RGAVIKPSAATPALMVHKGRAVVFESSDDFHKRIDDESLDIDETCVMVLKNCGPKGYP
                     GMAEVGNMPLPPKVLRKGVTDMVRISDARMSGTAYGTVVLHTAPEAAAGGPLAIVRSG
                     DMIELDVPNRRIHLDISDQELARRLAQWKAPEPPLKSGYWKLYVDHVLQADEGADLDF
                     LVGKRGAFVPKDNH"
BASE COUNT         1222 a         2248 c         2196 g         1185 t
ORIGIN      
        1 ggccttgcac atgcccgcga aagcctcctc gatttcggcg atgtcctgca ccgtggcgcg
       61 ctcggccgcc atccgcacgg cggcgggttc gaccacgcgc cgcagatcct ggaggtcgcg
      121 caggaactcc ggggtgaggc cggccttcgc ctgccacagg atgacgtcgg gatcgaacca
      181 gttccagtgc tcctcgggca gcacgcgcgt gccgaccttc gggccggtgc tcaccagccc
      241 cttgcccacc agcgacttca ccgcttcgcg aatcacagtc cggctcacgc ccagggcctc
      301 gcccagccgc ggctccggcg gaatcgacgc gcccggccgg tagcggccgg cgacgatcgc
      361 ctcgcccagg tgatccaggg tattgccgtg cacgttcttc atcgtggcca tcgaaaccaa
      421 tggtactacc atatgatctt tctccggtcc ggattttccc taaacgggct cttgttcggg
      481 tgacgccgcg tttctttcgt gctacaagat cggggctttc gtcaacgcgc ccgggagaac
      541 atggctgagg ttcaaatcaa gggagtcgag aaatatttcg gcgccacgca catcatccgc
      601 ggcgtggata tcgacatgga ggacggtcag ttcaccgtcc tggtcgggcc gtcgggctgc
      661 ggcaagtcca cgctgctgcg catgatcgcg gggcttgagg aaatcaccag cggcgagatc
      721 ctgatcggtg ggcgcgtggt gaacaacatg atgcccaagg aacgcgacat cgccatggtg
      781 ttccagaact acgctctgta cccgcacatg acggtgcgcg acaacatggc attcagcctg
      841 atgctggcgc ggcagcccaa ggcggtggcc gacgagcgcg tgcgcaaggc ggcggacatc
      901 ctcggcctca acgagctgct cgaccggtat cctcgccagc tctcgggcgg ccagcggcaa
      961 cgcgtcgcca tgggccgctg catcgtgcgc gacccgcagg tcttcctgtt cgacgagccg
     1021 ctgtcgaacc tggacgccaa gctgcgggtg cagatgcgca ccgagatcag ggaactgcac
     1081 cagcgcctga agacaacgtc ggtgtacgtc acgcacgacc agatcgaggc gatgacgatg
     1141 gccgacaaga tcgtcgtcat gcgcgacggt atcgtggagc agaccggcaa tccgctcgac
     1201 ctctatgacc atccggccaa tcagttcgtt gccggtttca tcggctcgcc ggcgatgaac
     1261 ttcctccccg gcacgctccg gcgctctaat gccgggatgt ccgtcgaact caatggcggc
     1321 gcccggctgg cggcgccacc cggcacgccg gacgccgacg gccagagcgt cgtgttcggc
     1381 acgcgccccg aacacctgac gctttcggat aacggcggca ttccggcacg cgtggtcgtg
     1441 atggagcctt cggggatgga cacgttcatc gtctgtcgcc atgaggagat gcagctctcg
     1501 gccgtgttcc gcgagcgcca cgactttgcc ccgggcagca ccatccgttt gcagcccgac
     1561 ctgcagcgcg cgcatctttt cgatgcaggc accggccggc ggctggctgc atgagccctt
     1621 cgccgcgacc gattacctcg acccactctg ccacaacagg agaacagcaa tgaccgactt
     1681 caatcgacgc aaattcctcg aaggctccgc tggcgttgcc gcggcggcca cattgggcgc
     1741 gggcgcgatc ttcgcgccgg cggtgcatgc acaggcctgg tccaaccagc ccgagaaggg
     1801 cgccaagctg cgcgtgctgc gctggagccg cttcgtgcag ggcgacatcg accagtacat
     1861 gaagaacgtg gccgcgttca ccgccaagac cggcattgaa gtccgggtgg acaacgaagg
     1921 ctgggaagac gtgcggccga aggccgcggt ggccgccaac acgggcgccg gccccgacat
     1981 catcctgtcg acgcacgacg atgccaacct gtacccggag aagctggtcg acgtgaccga
     2041 cctggccaac tacctcggcc gcaagtacgg cggctggtac cccgtgggcg aggcctacct
     2101 gcgacccaac ggcaagaact ggatcggcgt gccgctcggc tgcgccggca acaccctggt
     2161 ctaccgcgag agccaggtca aggcggcggg cttcgacaag ttcccgcgcg acaccgacgg
     2221 cttcctgcgg ctgatgaagg cgctgaagga gaaaggcacg ccgggcggca tggcactggg
     2281 caacgccacc ggcgacggaa acgtgtggac gcactggctg ctgtgggcct tcggcggtca
     2341 gctcgtcgac aagaacaaca aggtcgtcgt caacagcccg cagacggtca aggcgctgga
     2401 gtacgccagg gagctgtacc agaccttcgt gccgggcacg ctgtcgtggc tcgacccgaa
     2461 caacaacaag gctttcctcg acggccaggt cagcgtgacg cccaacggca tctcgatcta
     2521 ctacgccgcg aagacctcga aggatgcgaa gctgcaggaa atggcgaagg acatcaatca
     2581 cgtcaacatg ccgatcgggc cggccggcgt gcccaccgaa ctgaacttgt tcttcaacca
     2641 gatgatcttc aagcacagca agtacccgaa ggcggccaag gagttcatcc gcttcatgat
     2701 ggaagaggag cagtacggcc cgtggatgca ggccgcgatc ggctacgtga cgcagccgct
     2761 ggtggcctac gagaagaacc cgatctggac ggtggatccc aagcacacgc cgtaccgcga
     2821 ctcgatgaag atcatgcgcc cgatcgggta cgccggaaag ctgggttatg catcggccgg
     2881 cgccgcagcc gacttcatcg tcgtcaacat ggtggccgag gcgggcagcg gctccaagac
     2941 gccgcaggaa gctgcggaac gggcccagaa gcgcgctgag cgctattaca aggtctgacc
     3001 gccggcatgc cagccgatcc gacgcagcgc ccgcgcgaaa caggtggccc cacggccgcg
     3061 ctgggcgcgt ccgccggatc ggcggcttcc ccgcgtgacc ggcgcccttc caccctggaa
     3121 agactccaga acagccgcaa cgccctcggg ttcattttcc tgttccccgc ggcgctgctg
     3181 ctggtgctgt tcctgaccta cccgctcggg ttgggcgtct ggctcggctt cacggatacg
     3241 aaagtgggac ggccgggcgt gtgggtcggg ctggaaaact atgaattcat cgcgcaggac
     3301 gccgtcacgc agctggccct gttcaacacg ctgttctaca cgttcgtggc cagcgtgctg
     3361 aagttcttcc tcggcctgtg gctcgcggtg ctgctgaacc ggaacatccg cctcaagacc
     3421 ttcttccggg cagtgatcct gctgccctac atcgtgccca ccgccctgtc ggcgatcgcg
     3481 ttctggtgga tctacgacgc gcagttctcg gtgatctcgt gggcgctctt caagctcggg
     3541 ctgatcgacc gctacatcga ttttctcggc gagccttgga acgcgcggct gtccgtcatc
     3601 gcggccaacg tgtggcgcgg cgtgccgttt gtcgcgatca cgctgctggc ggggctacag
     3661 acgatctcgc cgtcgtacta cgaggcgtcg gccatcgacg gcgccacgcc gtggcagcag
     3721 ttcaggcacg tcacgctgcc gctgctgacg ccgatcatcg cggtggtgat gacgttctcg
     3781 gtgctgttca cgttcaccga tttccagctc atctacgtca tcacgcgcgg cggcccgctg
     3841 aacgccactc atctgatggc cacgctgtcg tttcagcgcg ccatccccgg cggcgcgttg
     3901 ggcgaaggcg cagcgatctc catcgccatg gtgccgttcc tgctggcggc catcatgttc
     3961 agctacttcg gcctgcagcg ccgcgcgtgg cagcaagggg gctcggacaa atgaaggctg
     4021 acgacaccat cgacagcgcg gggatgacct acctcgagac gccgctgcgc aaggccgtgg
     4081 tggtgtacct gccgctggcc gtgttcgtgt tcgtgctgct gttcccgttc tactggatgg
     4141 gcatcaccgc cttcaagccc aacaatgagc tgctctcgcg cgaaggcaat ccgttctggg
     4201 tgatcgcgcc gacgctggcg catttcaaga agctcctgtt cgacacgtcc tatccacagt
     4261 ggatgtggaa cacggtgctg gtttccgtgg tggcgacgtt cttctcgctg gcggcggcgg
     4321 tgttcgccgc ctatgcgatc gagcggcttc gcttctcggg cgccaagcag gtcgggctgg
     4381 cgatcttcct ggcctatctc gtgccgccgt ccatcctgtt cattccgctg gcctccgtgg
     4441 tgttccagct cgggctgttc gacacgcggc tggccctgat cctgacttac ccgaccttcc
     4501 tgatcccgtt ctgcacctgg ctgctgatgg gctattttcg ctccatcccc tacgagctgg
     4561 aggaatgcgc cctgatcgac ggcgccagcc gctggcagat cctgaccaag atcgtgctgc
     4621 cgctggccgt tcccgggctg atctcagccg gcatcttcgc gttcacgctg tcgtggaacg
     4681 agttcatcta cgccctcacg ttcgtttctt ccagcgaaat caagaccatc ccggtgggcg
     4741 tcgtcacgga actggtcgaa ggcgacgtct accactgggg atcactgatg gccggcgctc
     4801 tgctcggctc gctcccggtg gcattcattt attcgttttt cgtcgagcac tacgtctcgg
     4861 ggctgacggg ctctgtcaag gaatgaggcg gtcgcgggct cggcagtgcc ggctttcgaa
     4921 cgcatcccag ccttgcctcg cgaggcgccc cggacatttt ttccattaaa tgacacaagc
     4981 atgagcaact cccacccccc caggaaaaaa cccgaggaac tgcgcagcca gcagtggttc
     5041 ggccgccagg accgcgacgg cttcaattac cgcagctggg tcaagggcaa gggcgtgccg
     5101 cacgaccagt tcgatggccg tccggtcatc ggtatctgca acacgttcag tgagttgacg
     5161 ccatgcaaca gccacttccg caccctggcc gagcaggtga agatcggcgt ctacgaggcc
     5221 ggcggcttcc cgctggaatt tcccgtgatg tctttgggcg agacgctgct gcggcccacg
     5281 gcgatgctgt accgcaacct cgccagcatg gacgtggaag agagcatccg cggcaacccg
     5341 atcgacggcg tggtgctgct gatgggctgc gacaagacca cgccctcgct ggtgatgggc
     5401 gcgtcgagtg tcgacatccc gacgatcggc gtgtccggcg gcccgatgct caatggcaaa
     5461 tggcgcgggc aggaactggg ttcgggcacg ggtgtgtgga gcatgagcga acaagtgcgc
     5521 gccggcacgc tcaagctgca ggatttcttc gaggccgaga gctgcatgca ccgcagccac
     5581 ggccactgca tgacgatggg aaccgcctcg accatggcct gcatggtcga agcactgggc
     5641 atcggattgc cgggcaatgc ggcctatccg gctgtcgacg gccggcgcaa cgtgttggca
     5701 cgcatggcgg gccgccgcat cgtggagatg gtccatgaag acctgaagct ctcgaagatc
     5761 ctgacgcgcg aggccttcga gaacgccatc aagacgctgg ccgcgatcgg cggctccacc
     5821 aacgcggtga tccacctgat cgcgattgcc cggcgcatcg gcgtcgagct ggcgatcgag
     5881 gacttcgacc ggctcgccag cgacctgccg tgcctggtga acctgcagcc ctccggcgag
     5941 cacctgatgg aagacttctg ctacgcgggt ggcttgccgg tggtgatgaa ggaaatcgcg
     6001 cggcacctgc acctggatgc catcaccgcg aacggcagga aagtaggcga gaacattgcc
     6061 gacgcggtga actacagcac cgaagtcatc cagcctttgg cgcggccctt caaggagaag
     6121 gccggcatcg cggtgctgcg cggcaatctg gcgccccgcg gcgcggtgat caagcccagc
     6181 gccgccacgc cggcgctaat ggtgcacaag ggacgggccg tggtgttcga gagcagcgac
     6241 gattttcaca agcgcatcga cgacgagagc ctggacatcg acgagacctg cgtcatggtg
     6301 ctgaagaact gcggccccaa gggctacccc ggcatggccg aggtcggcaa catgccgctg
     6361 cccccgaaag tgctgcgcaa gggcgtcacc gacatggtcc gcatcagcga tgcgcgcatg
     6421 agcggcaccg cctacggcac ggtggtcctg cacacggcgc cggaagcagc cgcgggcggg
     6481 ccgctggcca tcgtgcgaag cggcgacatg atcgagctgg acgtgcccaa tcgccgcatc
     6541 cacctggaca tcagcgacca ggaactggcg cggcggctgg cgcaatggaa agcgccggag
     6601 ccacccttga agtcgggcta ctggaaactg tacgtcgacc atgtgctcca ggccgacgaa
     6661 ggcgccgacc tggatttcct ggtcggcaaa cgcggcgcgt tcgtgccgaa ggacaatcat
     6721 tgatggacat gcggatcaag cctgccccgg actccgatcc ggggtccgca atgacagcat
     6781 gaaaaatggc ggcgaccgcc acttcgacgt cgttgccctg ggcgaaggga tgctggaatt
     6841 caaccagacc g
//
LOCUS       DASWIY010000388         4728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1014602, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000388 DASWIY010000000
VERSION     DASWIY010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014602"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /gene="mrdA"
                     /locus_tag="VGA59_13815"
     CDS             complement(<1..1368)
                     /gene="mrdA"
                     /locus_tag="VGA59_13815"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX9720798.1"
                     /translation="MTELRNVEADLARFRVRVLVASIVVVVAFLLLVSRLVYLQVVRH
                     EDLREQAENNRISIVPVVPNRGLILDRNGIVLATNFSAYTLEIMPARLTGSLDKTIEE
                     LSQVVEIQPRETRRFRKLLEESKSIDSLPIRTRLTDEEVARFAAQSYRFPGVDIKARL
                     FRNYPWGELASHVVGYIGRVNQAEKKAMEEWDEDDQANYRGTEYIGKLGVEQAFEKQL
                     HGIAGVEQVETSAGGRAVRSLAYRPATPGNTVMLAIDIKLQKLVEDMYGDRRGALVAL
                     DPKTGEVLAFVSKPTFDPNLFVDGIDSESWQMLNESIDKPLLNRAMRGTYPPGSTYKP
                     FMAMAALKTGKRSPTHVIHDGGSWSFGGHVFRSHGDGLGAVDMHTSIVKSSNVYYYTL
                     ANELGVDTMHDFMKTLGFGQFTGIDLQGELRGVLPSQAWKRGYFKRPEQQKWYAGETI
                     SLGIGQ"
     gene            complement(1591..2109)
                     /gene="mreD"
                     /locus_tag="VGA59_13820"
     CDS             complement(1591..2109)
                     /gene="mreD"
                     /locus_tag="VGA59_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX9720799.1"
                     /translation="MIMRPGQQLLLPANPVFIWGSLLLALLANMLPLGRTPWLPDFLA
                     LALVFWSVHQPLRVGIGAAFMFGLVMDVHQAALLGQHALAYTVLSYFAITIHRRLLWF
                     SVPSQAVQVLPLFVAAHAIELAVRMLAGGIFPGWTVFLTPLIEALLWPVVSVVLLVPQ
                     RMAPDPDENRPL"
     gene            complement(2106..3026)
                     /gene="mreC"
                     /locus_tag="VGA59_13825"
     CDS             complement(2106..3026)
                     /gene="mreC"
                     /locus_tag="VGA59_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX9720800.1"
                     /translation="MPLGTLDRTPPPFFKQGPSAVSKLMVCSALALFLMVADTRFRVA
                     QPLRAVVATVLYPLQWLAMRPVLLVRYGAQYFESLESAQRWEEAARRKLAQQSHRANQ
                     VEQLTLENERLRKLLELRERVNTPSQAAEVLYDAADPYTRKVVIDKGLAQGIQAGSPV
                     IDESGVLGQVTRVYPLVSEVTLLTDREQAIPVLNARTGARSVAYGDPSSQGGALELRF
                     MAGNADVKPGDVLTTSGVDGVYPPGLPVARVAKVERRPDSAFARITCSPQALMDGARH
                     VMVLAPISAQVLPRPGQDDAAAPARKGVRK"
     gene            complement(3067..4110)
                     /locus_tag="VGA59_13830"
     CDS             complement(3067..4110)
                     /locus_tag="VGA59_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX9720801.1"
                     /translation="MFGSFRRYFSTDLAIDLGTANTLIYVRDKGIVLDEPSVVAIRHE
                     GGPQGKKTIQAVGSEAKAMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIKMVHPR
                     GIFKPSPRIIICVPCGSTQVERRAIRESALGAGASEVYLIEEPMAAAIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGMVYKGSVRVGGDKFDDAIISYIRRNYGMLIGEPTAEA
                     IKKNIGSAFPGSEVKEMEVKGRNLSEGVPRSFTISSNEILEALTDPLNNIVSAVKNAL
                     ESTPPELGADIAERGMMLTGGGALLRDLDRLLAEETGLPVLVAEDPLTCVVRGCGIAL
                     ERMERLGSIFTSE"
     gene            4325..4624
                     /gene="gatC"
                     /locus_tag="VGA59_13835"
     CDS             4325..4624
                     /gene="gatC"
                     /locus_tag="VGA59_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX9720802.1"
                     /translation="MALTSQDIARIANLARLELSAAESERMLTQINGFFDIVEKMRAV
                     DTAGIEPLSHPVAAFQDVALRLREDVVSEQNQREANQRSAPAVERGLFLVPKVIE"
BASE COUNT          798 a         1540 c         1557 g          833 t
ORIGIN      
        1 ctgcccgatg cccagcgaga tggtctcgcc cgcataccac ttttgctgct cgggccgctt
       61 gaaatagccg cgcttccagg cctggctcgg cagcacgccg cgcagctcgc cctgcaggtc
      121 aatgcccgtg aactggccga agcccagcgt cttcatgaag tcgtgcatcg tgtccacacc
      181 cagctcgttg gccagcgtgt agtagtacac gttgctggac ttcacgatgc tggtatgcat
      241 gtccaccgca cccaggccgt ccccgtggct gcggaacaca tggccgccga aactccagga
      301 gcccccgtcg tggatcacgt gggtgggcga tctcttgccg gttttcaagg ccgccatcgc
      361 catgaagggc ttgtaggtgg agcccggcgg gtaggtgccg cgcatcgcgc ggttgagcag
      421 cggcttgtcg atcgactcat tgagcatctg ccagctttcg ctgtcgatgc cgtccacgaa
      481 caggttgggg tcgaatgtgg gtttgctgac gaaggccagc acctcgccgg tcttggggtc
      541 cagcgccacc agcgcgccgc ggcgatcgcc gtacatgtcc tccaccagct tttgaagctt
      601 gatgtcgatc gccagcatca ccgtgttgcc gggggtagcc gggcggtacg ccaggcttcg
      661 caccgcgcgt ccgccggctg aagtctcgac ctgttcgacc cccgcgatgc cgtggagctg
      721 cttctcgaac gcctgctcca cgcccagctt gccgatgtat tcggtgccgc ggtagttggc
      781 ctggtcgtcc tcgtcccatt cctccatggc ctttttttcg gcctggttga cgcggccgat
      841 gtagccgacc acgtggctgg caagctcgcc ccagggatag ttgcggaaca gccgggcctt
      901 gatgtcgact ccggggaagc gatagctctg ggcggcgaaa cgggccacct cctcgtcggt
      961 caggcgggtg cggatcggca gcgaatcgat gctcttggac tcctcgagaa gtttcctgaa
     1021 gcgccgcgtc tcgcgcggct ggatctcgac gacttgcgac agctcctcga tcgtcttgtc
     1081 cagggaaccg gtcaggcggg cgggcatgat ctccagcgta taggccgaga aattggtggc
     1141 cagcacgatg ccgttgcggt ccaggatcag gccccggttg ggaaccaccg gcacgatcga
     1201 gatgcggttg ttctcggcct gctcgcgcag gtcctcgtgc cgcaccacct gcaggtagac
     1261 cagccggctg accagcagca ggaacgccac cacgacgacg atgctggcca ccagcacccg
     1321 cacgcgaaaa cgcgcgagat cggcttcgac attgcgcagt tcagtcatgg gacttttgat
     1381 cagttgcgga cagggcaaca ggctcgcgcc ggcgcgcggt gcttagttgg tcagtcccgc
     1441 aaagcttttg atcagttggg gacagagcag catgcttgcg ctggcgcgcg gagcttggtt
     1501 ggtcggtccc gcaaggcttt tgatcagttg gggacagagc aacacgcccg cgctggcgcg
     1561 cggagcttag ttggtcagtc ccgcaaagtt tcataagggc ctattctcat ccgggtcggg
     1621 tgccatgcgc tggggcacca ataacaccac gctcacgacc ggccacagca gcgcttcgat
     1681 cagcggggtc aggaaaaccg tccaaccggg aaaaatgccg ccggccagca tccgcacggc
     1741 cagctcgatc gcatgggccg ccacgaaaag cgggagcacc tgcaccgcct gcgacggtac
     1801 gctgaaccac agcaggcgcc ggtggatcgt gatcgcgaag tagctcagca cggtgtaggc
     1861 cagcgcgtgc tgccccagca gcgccgcctg gtgcacgtcc atgacgaggc cgaacatgaa
     1921 agccgcgcct atgcccacgc gcaggggctg atgcacgctc cagaacacca gggccagggc
     1981 caggaagtcc ggcagccacg gcgtgcggcc cagcggcagc atgttcgcca gcagcgccag
     2041 cagcaggctg ccccagataa agacgggatt ggccggcagc agcagctgct gcccgggacg
     2101 catgatcact tgcgcactcc tttcctagcc ggcgcggcgg catcgtcctg gccgggccgg
     2161 ggcagcacct gcgccgagat cggtgccagc accatgacat ggcgcgctcc gtccattagg
     2221 gcctggggcg aacaggtgat gcgggcaaaa gcggagtcgg ggcggcgctc caccttggcg
     2281 acgcgcgcca ccggcaggcc cggcgggtag acgccgtcga caccgctggt ggtgagaacg
     2341 tcgcccggct tcacgtcggc gttgccggcc atgaagcgca gctcgagcgc gccgccctgc
     2401 gatgagggat cgccatacgc gacactgcgt gcgccggtgc gagcgttgag cacgggaatc
     2461 gcctgctcgc ggtccgtgag caacgtgact tcgctcacca gcgggtacac ccgcgtcacc
     2521 tggccgagca cgcccgattc gtcgatcacc ggcgagccgg cctggatgcc ctgggccagg
     2581 cccttgtcga tcacgacctt gcgggtgtag ggatcggccg cgtcgtacag cacctcggcc
     2641 gcctggctgg gcgtgttgac gcgttcgcgc aactccagga gcttgcgcag ccgttcgttc
     2701 tccagggtga gctgctcgac ctggttggcg cgatgggact gctgcgccag tttgcgccgc
     2761 gccgcttctt cccatctctg ggcactctcg agcgattcga aatactgcgc gccgtagcgc
     2821 accagcagca ccggccgcat ggccagccat tgcagcgggt acagcacggt ggcgacgacc
     2881 gcccgcaggg gctgggcgac gcgaaaacgg gtgtccgcga ccatgaggaa aagcgccagg
     2941 gcgctgcaca ccatgagctt ggacacggcc gaggggccct gcttgaagaa gggcggcggc
     3001 gtccggtcga gggtacctag tggcatggcc gatggccctc cctgcccgtg tgcgggaggg
     3061 agggaactac tcgctggtga agatcgatcc gagccgctcc atgcgctcca gcgcgatgcc
     3121 gcagccgcgc accacgcagg tcagcgggtc ttcggccacg agcaccggca ggcccgtttc
     3181 ctcggccaac aggcggtcca ggtcgcgcag cagggcgcct ccgcccgtca gcatcatgcc
     3241 gcgctcggcg atgtcggcgc ccagttccgg cggcgtgctc tccagcgcgt tcttcacggc
     3301 cgacacgatg ttgttcagcg gatcggtgag cgcttccagg atttcgttgg aggaaatggt
     3361 gaaggaacgc ggcacgcctt cggacaggtt gcggcccttg acttccattt ccttgacttc
     3421 gctgcccgga aaggccgagc cgatgttttt cttgatcgct tcggcggtgg gctcgccgat
     3481 cagcatgccg tagttgcggc ggatgtagct gatgatggcg tcgtcgaact tgtcgccgcc
     3541 gacccgcacg ctgcccttgt agaccatgcc gcccagggaa atgacgccga cctcggtggt
     3601 gccgccgccg atgtccacca ccatcgagcc cgaggcttcc gaaaccggca ggccggcgcc
     3661 gatggcggcg gccatgggtt cctcgatcag gtacacctcg ctcgcgccgg cgcccagcgc
     3721 cgattcgcga atggcgcggc gctcgacctg ggtcgagccg cagggcacgc agatgatgat
     3781 gcgcgggctg ggcttgaaga tgccgcgcgg atggaccatc ttgatgaact gcttgagcat
     3841 ctgctccgtg acggtgaagt cggcgatcac gccgtccttc atcggccgga tggcctcgat
     3901 gttgcccggg accttgccga gcatggcctt ggcttcggag ccgacggcct ggatggtttt
     3961 cttgccctgg ggcccgcctt cgtggcgaat cgccaccact gatggttcgt ccagcacgat
     4021 ccccttgtcg cgcacataga tcaatgtgtt cgcggtaccc aggtcaatgg ccaggtcggt
     4081 cgaaaagtac cgacggaacg atccgaacat caggaagtcc tctcgcgagc aaggcaggca
     4141 tgcgcgcctg ccctcgccgc atcaatcatg ttatttgggc cgaaacccgc tttttcctgc
     4201 aaataccggc ggtttcgcgg atgttgcgct aaaggcggga taatacccga tccccttcga
     4261 atagctggtc cgtgccgggc caaaatatcg ggtttacatc tgtttcgccg cgccttttcc
     4321 cgccatggca ttgacctccc aagacatcgc ccgaatcgcc aatctcgcca gattggaatt
     4381 aagcgccgcc gagagcgagc gcatgctgac ccaaatcaac ggcttcttcg acattgtcga
     4441 gaagatgcgc gccgtggaca cggccggcat cgagccgttg tcgcatccgg tggcggcctt
     4501 ccaggacgtc gcgctgcggt tgcgcgagga cgtggtgagc gagcagaacc agcgcgaggc
     4561 caaccagcgc agcgcccccg ccgtcgagcg cgggctgttt ctcgtgccga aggtgatcga
     4621 atgagcgcga cggcgagggg ccccgcttgc ggggatcgac gcagcgaatt cgatcgggct
     4681 gccgacacca ggagaatgat gatcaccgca agagcttcgg aggcagtt
//
LOCUS       DASWIY010000389         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1015316, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000389 DASWIY010000000
VERSION     DASWIY010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015316"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1367
                     /locus_tag="VGA59_13840"
     CDS             99..1367
                     /locus_tag="VGA59_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="URC4/urg3 family protein"
                     /protein_id="HEX9720803.1"
                     /translation="MKGTDFISPHAQAQAAAEILRTTTAVRDRAKLLLSRARQGESPW
                     FVVDEGFLESAAGEVVAVTRRRYPKLHVPLHSRWRHFEAGGIDRKAQLDRLLAHLPRP
                     LRGHALIDLAFVSVLLDAGAGPGWKYVEPATGQTFTRSEGLAVASFHAFVAGLFSSDT
                     DRPMRVDSAGLRGLHTDHLASAFQVSEANPLVGIEERAILLRRLGEVMAAQPEAFGED
                     GRPSGLFDTMVSPLGPEVPPTADVAAHDILSEILMTLSDIWPFGNSIGTVPLGDCWRH
                     SAVRGAGLSDGWMPFHKLSQWLTYSLIEPFAWSGVSVLGLDELTALPEYRNGGLLIDS
                     GLLRLRDESVALEIWQPGDEIVVEWRALTVALMDEVARAVRHQLRLSEAQLPLACVLE
                     GGTWAAGRELAQRHRNGLPPLQVASDGTVF"
     gene            1390..2019
                     /gene="upp"
                     /locus_tag="VGA59_13845"
     CDS             1390..2019
                     /gene="upp"
                     /locus_tag="VGA59_13845"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX9720804.1"
                     /translation="MANLHLIEHPLVQHKLTLMRKKEASTNSFRRLLNELAMLMAYEV
                     TRDMAMQEVEIETPLEKMKARVIDGKKLVFVSILRAGTGILDGMLSVVPGARVGHIGL
                     YRDPKTLTAVEYYFKMPQHMSDRDVIVVDPMLATGNSAIAAVERLKELSPKSIKFVCL
                     LTCPEGIAAFQGAHPDVPIYTAAIDRQLNDHGYILPGLGDAGDRIFGTK"
BASE COUNT          401 a          782 c          779 g          380 t
ORIGIN      
        1 gtcgagatgg acgccaaggt ggcggccggc tatttcacgc ccgggcgcgt gccggacgcg
       61 caggaactca agcaggccaa gggccggggc ttcgataaat gaagggcacc gacttcatct
      121 cgccccatgc gcaggcgcag gctgccgcgg aaattcttcg caccaccacc gccgtgcgcg
      181 atcgtgcgaa gctgctgctg tcgagagcgc gccagggcga gtcgccctgg ttcgtggtgg
      241 acgagggatt tctcgagtcc gccgccggcg aggtcgtggc ggtcacccgg cggcgctacc
      301 ccaagctgca cgtcccgctg cacagccgct ggcgccattt cgaggccggt ggcatcgacc
      361 gcaaggccca gctcgaccgc ttgctggcgc atttgccgcg gcccttgcgc ggccacgcct
      421 tgatcgatct ggccttcgtg agcgtgctgc tcgacgccgg cgccggaccc ggatggaagt
      481 acgtggagcc ggccacgggc cagaccttca cccggtcgga gggactggcg gtggccagct
      541 ttcatgcgtt tgtcgcgggg ctgttttcca gcgacacgga ccgcccgatg cgcgtcgact
      601 cggcgggcct gcgcggcctc cacaccgatc acctggcgtc cgccttccag gtcagcgagg
      661 ccaacccgct ggtcgggatc gaggaacgtg ccatcctgct caggcgattg ggcgaagtca
      721 tggcggcgca acccgaggcg ttcggcgagg acggccggcc cagcggcttg ttcgacacga
      781 tggtgagccc gctcggcccc gaggttccgc cgaccgcgga cgtcgccgcc cacgacatcc
      841 tgtcggagat cctgatgacg ctgtcggaca tctggccctt cggcaattcc attgggaccg
      901 tgccgctggg cgactgctgg cggcattcgg cggtgcgggg cgccgggctg tccgacggct
      961 ggatgccctt tcacaagctt tcgcagtggc tgacctattc gctcatcgag cctttcgcct
     1021 ggtcgggcgt cagcgtgctc ggactggacg agctcacggc cctgccggag taccgcaacg
     1081 gcggcctctt gatcgacagc ggcctgctgc gcctgcgcga tgaatccgtg gccctggaga
     1141 tctggcagcc aggggacgag atcgtggtgg aatggcgcgc gctgaccgtc gccttgatgg
     1201 acgaggtggc acgggccgtg cggcaccagc tgcgattgag cgaagcgcag ctgccgctgg
     1261 cctgcgtgct ggagggcggc acctgggccg ccggacgcga gctggcgcag cgtcatcgca
     1321 acggcctgcc gcccctgcag gtggcgagcg acggaacggt attttgaaaa gaagaacaag
     1381 gcaaggacaa tggccaacct ccacctgatc gaacatccgc tggtccagca caagctcacg
     1441 ctgatgcgca agaaggaggc cagcaccaac agcttccggc ggctgctcaa cgagctggcg
     1501 atgctgatgg cctacgaagt cacgcgcgac atggccatgc aggaagtcga gatcgagacc
     1561 ccgctggaaa agatgaaggc cagggtgatc gacggcaaga agctggtgtt cgtctccatc
     1621 ctgcgggccg gcaccggcat tctcgacggc atgctcagcg tggtgccggg cgcgcgcgtc
     1681 gggcatatcg gcctgtaccg cgatcccaag acgctcacgg cggtggagta ctacttcaag
     1741 atgccgcaac acatgagcga tcgcgacgtg atcgtggtcg atcccatgct cgccaccggc
     1801 aattccgcga ttgcggcggt ggagcggctg aaggagctca gtcccaagtc gatcaagttc
     1861 gtgtgcctgc tgacctgccc cgaaggcatc gcggcattcc agggtgcgca tccggacgtg
     1921 ccgatctaca ccgcggccat cgaccgccag ctcaacgacc acggctacat cctgcccggc
     1981 ctgggcgacg caggcgaccg gatcttcggc acgaaatagc gttgtcattg cgaagcgtgg
     2041 atgcccccgg atctggtccg gggcaggctc atcaagtccg gcatgacagg aaaaattctg
     2101 tcattgcggc cttgagccgc aatccatgac cccgaattca tggatgccgg gtcatgcccg
     2161 gcatgacgag cccatcggcc cgctagactc gcgtcgatgc cagaaaaacc aacaacaaca
     2221 cttgccgccg gactgctcct cgcctgcgcc gttgcgacag ccgcccccta cacccccgcc
     2281 aatgacaacg acgtcgtcga gcgcctgccc gctggtgcga cggacccttc cgtacgccgc
     2341 gt
//
LOCUS       DASWIY010000390         3126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2137145, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000390 DASWIY010000000
VERSION     DASWIY010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137145"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VGA59_13850"
     CDS             complement(<1..439)
                     /locus_tag="VGA59_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX9720805.1"
                     /translation="MASWIDRIPLWLRNALRPARPFYRAAQLWSDVGGGRMSAAISFY
                     GVLSLAPLMLLLVGVLGWWLDRELLETGLITQIGTIVGEQGGALIRQALDSAKEPRQG
                     VAASLIGFAVLLSGATGVFGELQAAFERLWVAGRDGMPERKWWH"
     gene            complement(652..>3126)
                     /gene="metH"
                     /locus_tag="VGA59_13855"
     CDS             complement(652..>3126)
                     /gene="metH"
                     /locus_tag="VGA59_13855"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX9720806.1"
                     /translation="EPDIARVPIMVDSSKWEVIEAGLRCIQGKGIVNSISMKEGVDAF
                     KHQARLVKRYGAAAVVMAFDEQGQADTYQRKIEICERAYRILVDEIDFPPEDIIFDPN
                     IFAIATGIEEHNNYAVDFINATRWIKQNLPGAKVSGGVSNVSFSFRGNDPVREAIHTV
                     FLYHAIQAGMDMGIVNAGMVGVYDELDPQLRERVEDVVLNRRPRLAPGEPDLSPGERL
                     VEIAESAKGAAKDDSKKNEWRAGTVEQRLSHALVSGITDFIVGDTEEAYQAVLAKGGR
                     PLHVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPYIEEEKKQQAAA
                     GEDVKAKGKIVIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCHEILARAKVEG
                     ADIVGLSGLITPSLEEMQYVAGEMQKDDHFRIRKIPLLIGGATTSRVHTAVKIAPHYE
                     GPVVYVPDASRSVSVAQGLLSEQASKYIAEVEADYEKVRNQHASKKQTPLWPLAKARA
                     NKTPIDWTGYTPPVPKFLGRRVFRNFDLAELAKYIDWGPFFQTWDLAGPFPAILKDEV
                     VGTEAVRVYADGQRMLKRLIEGRWLTASGVMGFWPANSVNDDDIALYTDESRRQVAMT
                     WYGLRQQTEKQAVEGRMRPSRCLADFVAPEGVAADYIGMFAVTAGIGVEKKEKYFLDD
                     HDDYSAIMLKALADRLAEAFAEALHERVRRDLWGYAQGESLSLEDQIGEKYRGIRPAP
                     GYPACPDHSVKRDMFDVLQAQDAGMGLTESMAMTPAASVSGFYLSHPDSTYFNVGKIG
                     DDQLKDQARRRGADVAQLQRLLAPNL"
BASE COUNT          501 a         1080 c          973 g          572 t
ORIGIN      
        1 catgccacca tttccgctcc ggcatcccgt ctctgccagc aacccaaagg cgctcgaagg
       61 cggcctgcag ctcgccgaac acgccggtgg caccggacaa cagcacggcg aaaccgatca
      121 gggaagccgc cacaccctgg cgcggctcct tggcactgtc cagagcctgg cgaatcaggg
      181 cgccgccctg ctccccgacg atggtgccga tttgcgtgat caggccggtc tcgagcagtt
      241 ccctgtcgag ccaccaaccc agcacaccca ccagcagcag catcaggggc gccaggctga
      301 gcacgccgta gaaggatatc gcggcgctca tgcgcccgcc gccaacatcg ctccagagct
      361 gggcggcgcg atagaaggga cgggccgggc gcaaggcatt gcgcagccac agcggaatgc
      421 gatctatcca tgaagccatg cgcaaaggct atggacgcct ggcgctgccc ggcataggcc
      481 gcgcacgccc aaccgcgtag gctgccgccg acgcactcca aaaacatgga tgccgggtca
      541 cgcccggcat gacaaccctc ccctgtcatt gcggacctga cccgcaatcc atctcacgcg
      601 gccggcgaga gctggatgcc ggtccatata gcccggcatg acagcctggc gctagagatt
      661 cggcgcgagc agccgctgca gctgcgccac gtcggcgccg cgccgccggg cctggtcctt
      721 cagctggtca tcgccgatct tcccgacgtt gaagtaggtg ctgtcggggt ggctcagata
      781 gaagccgctg acgctggccg ccggcgtcat cgccatgctt tcggtcaacc ccatgcccgc
      841 gtcctgcgcc tgcagcacgt cgaacatgtc cctcttcacg ctgtggtcgg ggcaggccgg
      901 gtagcccggc gccggccgga tgcctcggta tttctcgccg atctggtctt ccagcgacag
      961 cgactcgccc tgcgcgtagc cccagagatc ccttctgacg cgctcgtgca gcgcctcggc
     1021 aaaagcctcg gccaggcggt cggccaaagc cttgagcatg atggccgagt agtcgtcgtg
     1081 gtcgtccagg aagtactttt cctttttctc cacgcctatg cccgcggtca ccgcgaacat
     1141 gccgatgtaa tcggcggcca cgccctcggg cgcgacgaaa tcggcgaggc agcggctggg
     1201 ccgcatcctg ccttcgacgg cctgcttctc ggtctgctgg cgcagcccgt accaggtcat
     1261 cgccacctga cggcgcgact cgtcggtgta aagcgcgatg tcgtcgtcgt tgacgctgtt
     1321 ggcgggccag aagcccatga caccgctggc ggtgagccag cgcccttcga tcaggcgctt
     1381 gagcatgcgc tggccgtcgg catagacgcg cacggcttcg gtgcccacca cttcgtcctt
     1441 gaggatggcg gggaacggac cggccaggtc ccaggtctgg aagaacggcc cccagtcgat
     1501 gtatttcgcg agctcggcca ggtcgaaatt cctgaacacc cgacggccca ggaatttcgg
     1561 caccggcggc gtgtagccgg tccagtcgat cggcgtcttg ttggcgcgcg ccttcgccaa
     1621 aggccacagc ggcgtctgct tcttgctggc gtgctggtta cgcaccttct cgtagtcggc
     1681 ctcgacctcg gcgatgtatt tcgacgcctg ttcggacaac aggccttggg ccacgctcac
     1741 gctgcgcgac gcatcgggca catagaccac cggcccttcg tagtgcggcg cgatcttcac
     1801 cgccgtatgc acgcggctgg tggtggcgcc gccgatcagc agcgggatct tgcggatgcg
     1861 gaaatggtcg tccttctgca tctcgccggc gacgtactgc atttcctcca gcgacggcgt
     1921 aatcagcccc gacaggccga cgatgtcggc gccctccacc ttggcgcgcg ccaggatctc
     1981 gtggcagggc accatcacgc ccatgttcac cacttcgaag ttgttgcact ggagcaccac
     2041 cgtgacgatg ttctttccga tgtcgtgcac gtcgcccttg acggtggcga tgacgatctt
     2101 gcccttggcc ttgacgtcct cgccggctgc cgcctgctgc ttcttttctt cctcgatgta
     2161 ggggatgagg tgggccaccg cctgcttcat cacgcgcgcc gatttcacca cctgcggcag
     2221 gaacatcttg ccctggccga acaggtcgcc cacgatgttc atgccgtcca tcagcgggcc
     2281 ttcgatcacg tgcagcggcc ggccgccctt ggccagcact gcctgatacg cctcttcggt
     2341 atccccgacg atgaagtcgg tgatgcccga caccagggca tgcgacaggc gctgttccac
     2401 ggtgcccgcg cgccattcgt tcttcttcga gtcatccttg gcagcgccct tggccgactc
     2461 ggcgatttcg accagccgct cgcccgggga aagatcaggc tcgcccgggg cgagccgggg
     2521 ccggcgattg agcaccacgt cctccacccg ctcgcgcagc tgcggatcga gctcgtcgta
     2581 cacgcccacc atgccggcgt tgacgatgcc catgtccatg ccggcctgga tcgcgtggta
     2641 caggaacacc gtgtggatgg cctcgcgcac cgggtcgttg ccgcggaagg agaagctcac
     2701 attggacacg ccccccgaca ccttggcgcc cggcaggttc tgcttgatcc agcgcgtggc
     2761 gttgatgaag tcgacggcgt agttgttgtg ctcctcgatg ccggtggcga tcgcgaagat
     2821 gttggggtcg aagatgatgt cctcgggcgg gaaatcgatc tcgtccacca ggatgcggta
     2881 ggcgcgctcg cagatctcga tcttgcgctg gtaggtgtcg gcctgccctt gctcgtcgaa
     2941 ggccatgacc acggccgcgg cgccgtaacg cttgaccagc ctggcctggt gcttgaaggc
     3001 gtcgacgcct tccttcatcg agatcgaatt gacgatgccc ttgccctgga tgcagcgcag
     3061 gccggcttcg atcacctccc atttggacga atccaccatg atcggcacgc gggcgatgtc
     3121 cggctc
//
LOCUS       DASWIY010000391         3488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1373440, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000391 DASWIY010000000
VERSION     DASWIY010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373440"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /gene="aspS"
                     /locus_tag="VGA59_13860"
     CDS             complement(<1..719)
                     /gene="aspS"
                     /locus_tag="VGA59_13860"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX9720807.1"
                     /translation="MRTTYCGLVTEDLLGRTVTLCGWVNRRRDHGGVIFVDLRDREGY
                     VQVVCDPDRAEMFKAAESLRNEYCIQVKGLVRARPEGTVNENLKSGRIEVLCQDLAVL
                     NPSVTPPFQLDDESLSETTRLVHRVLDLRRPYMQNNLMLRYKVTMEVRKFLDANGFID
                     IETPMLTKSTPEGARDYLVPSRVHDGMFFALPQSPQLFKQLLMVAGYDRYYQITKCFR
                     DEDLRADRQPEFTQVDVEMSFP"
     gene            complement(751..1392)
                     /locus_tag="VGA59_13865"
     CDS             complement(751..1392)
                     /locus_tag="VGA59_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX9720808.1"
                     /translation="MHAVRKWFVAGLLVIVPVAITVAVLQWIIGTLDRTLLILPEAWQ
                     PDRLIGVHIPGFGVLLTLAILLSVGAVVSNFMGKKLITWGDTLVSRIPVVRSIYSSVK
                     QVSDTLFSPSGNAFRTAVLVQWPRPDVWTIGFVTGVPGGDVTNYLVGDYLSVYVPTTP
                     NPTGGYFVMLRKSDCVELKMSVDEALKYVISMGVVAPRVTDDDALPFRGGRVL"
     gene            complement(1400..1759)
                     /locus_tag="VGA59_13870"
     CDS             complement(1400..1759)
                     /locus_tag="VGA59_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX9720809.1"
                     /translation="MPIYAYKCGSCGHAKDVLQKLSDAPLTVCPSCGAAAFGKQLTAA
                     GFQLKGSGWYATDFKNGSKAAPASGADGAKPADATKDGAKPADGAAKPAETTPAATKT
                     DAPSAAGRGCGGAPACH"
     gene            complement(1848..3314)
                     /locus_tag="VGA59_13875"
     CDS             complement(1848..3314)
                     /locus_tag="VGA59_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX9720810.1"
                     /translation="MLITFVVLYLVASVAIGLYAAKRVKNTADYAVAGRSLALPVVIA
                     TTFATWFGSETVLGVSARFVSGGLGAVVEDPFGASMCLVLVGLFFAYRLYQKNLITLG
                     DYYRQRFGSTIEVLCSVMIIFSYLGWVAAQVTALGLVFNLLSQGAISVPVGMFIGISV
                     VLVYTLFGGMWSVALTDFVQMIVIGIGLLVIAWFAADLAGGAGKVIDYAATEGKFQFF
                     PTGDGKEWLFFFAAAITMMLGSIPQQDVFQRVMSSNSAETAVRGPVIGGTLYFLFALI
                     PMFVVTAAVLVMGPEAQALLQDDPQKVLPTLVMQHMPLMLQVAFFGALLSAIMSTASA
                     TLLAPSTAFVENILRNMRPHMSDKATLRAMRITVLVVSAVVLAYAIAMQGTSIYELVS
                     GAYQVPLVGAFVPLVFGLYWTRSTTQGALASVVLGLGTWLVFIATPSLSEAFPQQLAG
                     LLAALAGMIAGSLVPQGVKDHKGRVHHFEASTAPEQAA"
BASE COUNT          675 a         1124 c         1137 g          552 t
ORIGIN      
        1 gggaacgaca tctcgacgtc gacctgggtg aactcgggct gccggtcggc ccgcaggtcc
       61 tcgtcgcgga agcacttggt gatctggtaa taacggtcgt agccggccac catcagcagc
      121 tgcttgaaca gctggggcga ctgcgggaga gcgaagaaca tgccgtcgtg cacgcggctg
      181 ggcaccaggt agtcgcgcgc gccctctggc gtggacttgg tcagcatcgg cgtctcgatg
      241 tcgatgaacc cgttggcgtc caggaacttg cgcacctcca tcgtcacctt gtagcgcagc
      301 atcaggttgt tctgcatgta gggacggcgc aggtccagca cgcggtgcac caggcgggtg
      361 gtctccgaga ggctctcgtc gtcgagctgg aacggcggcg tgacggacgg attgagcacc
      421 gcgaggtcct ggcacagcac ctcgatcctg ccgctcttca ggttctcgtt caccgtaccc
      481 tcgggccgcg cgcgcaccag gcctttcacc tggatgcagt actcgttgcg caggctctcg
      541 gccgccttga acatctcggc ccggtcgggg tcgcacacca cctgcacata gccctcgcgg
      601 tcgcgcaggt ccacgaagat cacgccgcca tggtcgcggc ggcggttcac ccacccgcac
      661 agggtgacgg tccggcccag caggtcttcg gtcaccagcc cgcagtaggt ggttcgcatc
      721 ttgctttctg acataggaga aataccttat tcaaaggacg cgtccgccgc gaaacggcag
      781 cgcgtcatcg tccgttactc ttggagcgac gacgcccatc gagatcacgt acttgagcgc
      841 ctcgtcgacg ctcatcttca gttcgacgca atcgctcttg cgcagcatca cgaaatagcc
      901 gcccgtgggg ttgggcgtgg tcggcacgta gacgctcagg tagtcgccca cgaggtaatt
      961 ggtgacatcg cccccgggaa cgccggtgac gaagccgatg gtccagacat cgggccgggg
     1021 ccactgcacc agcacggcgg tgcgaaaggc gttgccgctg ggagaaaaca gcgtgtccga
     1081 aacctgcttg acgctggagt agatggagcg cacgaccggg atgcggctga ccagggtgtc
     1141 gccccaggtg atcagcttct ttcccatgaa gttgctgacc acggcgccca ccgacagcag
     1201 gatggccagc gtcagcaata cgccgaaacc ggggatgtgg accccgatca ggcggtcggg
     1261 ttgccaggct tcgggcagga tcagcagcgt gcggtccagc gttccgatga tccattgcag
     1321 caccgcgacc gtaatggcca caggcacgat gaccagcaag ccggccacga accattttcg
     1381 cactgcatgc atagcggtct cagtggcagg cgggggcgcc gccgcatcca cggccggcgg
     1441 ccgagggcgc atcggtcttg gtggcggccg gggtggtctc ggcgggtttg gcggcgccgt
     1501 cagccggttt ggcaccgtcc ttcgtggcgt cggcgggttt tgcgccgtcg gccccggagg
     1561 ccggcgccgc cttggagccg ttcttgaagt cggtcgcgta ccagccggat cccttgagct
     1621 ggaaacccgc ggcggtgagc tgtttgccga aagcggcggc cccgcaggac gggcagaccg
     1681 tcagcggcgc atcggagagt ttctgcagca cgtccttggc atggccacag gagccgcatt
     1741 tgtaagcgta gatgggcatg gcgctccagg cagttgggtg aaggtgcaaa gccttcaatt
     1801 ataggcgggc cccccgattt aacagcctgg cccgcctgcc gggcacttca cgccgcttgc
     1861 tcgggcgccg tgcttgcctc gaagtggtgt acacggccct tgtggtcctt caccccctgc
     1921 ggcaccagtg agcccgcgat catcccggcc agcgccgcca gcagccctgc cagctgctgc
     1981 gggaacgcct ccgacaacga gggcgtggcg atgaaaacca gccaggtccc caaccctaga
     2041 accaccgagg ccaatgcgcc ctgggtggta gagcgcgtcc agtacaagcc gaacaccagc
     2101 ggaacgaacg cgccgaccag cggaacctgg tacgcgccgg ataccagctc gtagatggaa
     2161 gtaccttgca tggcgatggc gtaggccagc acgacggcgg acacgaccag gaccgtgatc
     2221 cgcatcgccc gaagcgtcgc cttgtcgctc atgtgcggtc gcatgttgcg caggatgttc
     2281 tccacgaacg cggtgctggg cgccagcaac gtggctgaag cggtggacat gatcgccgac
     2341 aacagcgcgc cgaaaaacgc cacctggagc attaacggca tgtgctgcat caccagcgtc
     2401 ggcagcacct tttgcggatc gtcctggagc agcgcctggg cctcggggcc catcacgagc
     2461 acggcggcgg tgaccacgaa catggggatg agggcgaaca ggaaatacag ggtgccgccg
     2521 atcacggggc cgcgcaccgc cgtctcggcg ctgttggaag acatcacccg ctggaacacg
     2581 tcctgctggg ggatcgagcc gagcatcatg gtaatggccg ccgcgaagaa gaagagccat
     2641 tccttgccat cgccggtggg aaagaactgg aacttgccct cggtggcggc atagtcgatc
     2701 accttgccgg cgcccccggc caggtcggcg gcaaaccagg cgatcacgag gagcccgatc
     2761 ccgatcacga tcatctggac gaagtcggtc agcgccacgg accacatgcc cccgaacagc
     2821 gtgtacacca gcaccaccga aatgccgatg aacatgccca ccggaacgga gatcgcgcct
     2881 tgcgagagca ggttgaatac caggcccagg gccgtcacct gcgcggccac ccaaccgaga
     2941 tagctgaaga tgatcatcac cgagcacagg acctcgatcg tgctgccgaa gcgctggcgg
     3001 taatagtcgc ccagcgtgat caggttcttc tggtagagcc tgtaggcgaa gaacaggccc
     3061 accaggacca ggcacatgga agcgccgaac gggtcttcca ccaccgcgcc caggccgccg
     3121 gagacgaagc gggccgacac gcccagcacg gtttccgagc cgaaccaggt ggcgaaggtg
     3181 gtggcaatga ccaccggcag cgccaggctg cggcccgcga cggcgtagtc ggcggtgttc
     3241 ttgacgcgct tggccgcgta caagccgatg gccacggatg ccacaaggta cagcacaacg
     3301 aatgtgatca gcatggggtc tccgtaacga gtgaaagcct gattgcagcg gccgctctct
     3361 ccggccgatg cccgggatta tccaaaaaaa acgtactaga acaacctgac gcgaaccagc
     3421 agctgcatga cgatcaggcc caaggcaaaa ccgatgccgc cgtacaccag gccctgcagc
     3481 agccggtt
//
LOCUS       DASWIY010000392         5421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2137770, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000392 DASWIY010000000
VERSION     DASWIY010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137770"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="VGA59_13880"
     CDS             complement(<1..1583)
                     /locus_tag="VGA59_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9720811.1"
                     /translation="MFPEADAASPLLQNLNPEQRAAVTLPNEHALILAGAGSGKTRVL
                     TTRIAWLLSTGQVSPGGVMAVTFTNKAAKEMMTRLQAMLPVNVRGMWIGTFHGLCNRF
                     LRAHYKLANLPQSFQILDSQDQLSAIKRLMKQHNVDEERFPARQTQWFIAGSKEDGLR
                     PNAVEVRGEEDRKKVEIYQIYEEQCQREGVVDFGELMLRSYELLRDNDPIREHYQRRF
                     RHILIDEFQDTNRLQYAWIKMFADGNSVFAVGDDDQSIYAFRGARVGNMADFVREFDV
                     KHQIKLEQNYRSHSNILDSANELISHNKKRLGKNLRTTHGPGEPVRVYEAPTDLAEAQ
                     WMVEEMRHLVRGDPGSASGAGFPQGGLERHEIAVLYRSNAQSRVIETALFNAAVPYRV
                     YGGLRFFERAEIKHALAYLRLLENPHDDTSFLRIVNFPPRGIGARSVEQLQDAARAAG
                     CSLHDAVSATTGKAGANLTAFAAKIDQLREQTLNLTLREIIDMVLQASGLIEHYKSER
                     EGADRIENLEELVNAAESFV"
     gene            1617..2321
                     /locus_tag="VGA59_13885"
     CDS             1617..2321
                     /locus_tag="VGA59_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720812.1"
                     /translation="MNNLSRLIVPTLLLALHAGGLRAQPSCSSDGQPQPVGLLERFIN
                     ADCESCWSDPGTARPARGELALDWIVPGGRGDDAPLSAAASRDALARLEALRRAAPPR
                     AASSRQKVQRPARRLRVAQGPPINGYIGASIELRRGGEGPWKAWLLLVETIPAGSEGT
                     PVARNLVRNALQPAWDGKKALSKEEQRRLFEARPMNIPEGADPARLRVVGWVEDAHGR
                     IRAIAQSRCEPPGAKG"
     gene            2437..3414
                     /locus_tag="VGA59_13890"
     CDS             2437..3414
                     /locus_tag="VGA59_13890"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEX9720813.1"
                     /translation="MEIFDYDKVLLLPRKCRVESRSECDAGVELGGRSFRIPVVPANM
                     KTVVDEKLCLWLVQNGYFYVMHRFDFDNVVFMRKMKAQGAYGSISVGVKSADHTMVDQ
                     LAAEGLVPEYVTIDVAHGHADTVKTMIGHLKDKLPTAFVIAGNVATPEAVIDLENWGA
                     DATKVGVGPGKVCITRMKTGFGTGGWQLSALKWCARVATKPIIADGGIQEHGDIAKSV
                     RFGATMVMIGALLAGHEESPGQTVDVDGKLYKEYFGSASDFNKGEYRHVEGKRILEPV
                     KGKLADTLREMEEDVQSSISYAGGKKLMDIRKVNYVILGGDNAGEHLLM"
     gene            complement(3622..4479)
                     /locus_tag="VGA59_13895"
     CDS             complement(3622..4479)
                     /locus_tag="VGA59_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9720814.1"
                     /translation="MSTRYDGQWLDRMYNNRELVPAHPEHLSRWADASAEAVHTQTRE
                     LDVRYGGGPGEALDIFPAAQAGAPVLVFIHGGYWRALDKSDHSFVAPAFTREGACVVL
                     PNYALCPAVTIPQITMQLVQALAWTWRNIARHGGDPHRITVAGHSAGGHLAAMMLACL
                     WPAFDRDLPADLVKGALSISGLHDLEPIMHTPFLQPSLQLTHEQVRQASPARLPAPDH
                     GVLHTVTGGDESEEFQRQNRLIREAWGEERVPVCEALPGLNHFSVLDALVEPGQRLHQ
                     LALQLLRRY"
     gene            4586..5257
                     /locus_tag="VGA59_13900"
     CDS             4586..5257
                     /locus_tag="VGA59_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9720815.1"
                     /translation="MSAQLVRIATAPDLVDQVYRSLLDAVSDGSLAPGERITQEDIAA
                     RLAVSRQPVLQALRMLKKDGFLADAPGRGLMVTPLDPDWILKVYQVRGALDALAARLA
                     ASRRVRLDAKLIEHGRKAARGKDVKAMIDADMAFHNAIYAASRNPLIEQSAHQHWRHL
                     RRVMGAVLQQSRQRETVWDEHEAIARAIAAGDADKAARLIEQHSQHASKDLAARLTHV
                     LKTSA"
BASE COUNT          944 a         1756 c         1798 g          923 t
ORIGIN      
        1 acgaaactct cggccgcgtt gaccagttcc tccaggttct cgatgcggtc cgcgccttcg
       61 cgctcgctct tgtaatgctc gatcagcccc gacgcctgca gcaccatgtc gatgatttcg
      121 cgcagggtga ggttcagggt ctgctcgcgc agctggtcga tcttcgccgc gaaggcggtg
      181 aggttggccc ccgccttacc cgtggtggcg ctgacggcgt catgcagcga acagccggcc
      241 gcgcgcgccg cgtcctgcag ctgctcgacg ctgcgcgccc cgatgccgcg cggcgggaag
      301 ttcacgatcc gcaggaagct ggtgtcgtcg tgcgggttct ccagcaggcg caggtaggcc
      361 agtgcgtgct tgatctcggc gcgctcgaag aaacgcaagc cgccgtagac gcggtacggc
      421 acggcggcat tgaacaacgc cgtttcgatc acccggcttt gcgcgttgct gcggtagagc
      481 accgcgatct cgtgccgctc gagcccgccc tgggggaagc ctgccccgga cgctgatccg
      541 gggtcaccgc gcaccaggtg ccgcatttcc tccaccatcc actgcgcttc ggccagatcg
      601 gtgggcgcct cgtacacccg caccggctcg ccggggccat gggtcgtgcg caggttcttg
      661 cccagccgct tcttgttgtg gctgatcagc tcgttggccg agtccaggat gttgctgtgg
      721 ctgcggtagt tctgttccag cttgatctgg tgcttgacat cgaactcgcg cacgaagtcc
      781 gccatgttgc ccacccgcgc gccgcggaag gcatagatgc tctggtcgtc gtcgccgacg
      841 gcgaacaccg agttgccgtc cgcgaacatc ttgatccagg cgtactgcag ccggttcgtg
      901 tcctggaact cgtcgatcag gatgtggcgg aagcgccgct ggtagtgctc gcgaatcggg
      961 tcgttgtccc gcagcagctc gtagctgcgc agcatcagtt cgccgaagtc caccacgcct
     1021 tcgcgctggc actgctcctc gtagatctgg tagatctcga ccttcttgcg gtcctcctcg
     1081 ccacgcacct ccaccgcgtt gggtcgcagg ccatcttcct tgctgccggc gatgaaccac
     1141 tgggtctgcc gggccggaaa ccgttcctcg tccacgttgt gctgcttcat cagccgcttg
     1201 atcgccgaca gctggtcctg cgagtcgagg atctggaagc tctgcggcag gttggccagc
     1261 ttgtaatggg cgcgcaggaa gcggttgcac aggccgtgga aagtgccgat ccacatgccg
     1321 cgcacgttca ccggcagcat ggcctgcagc cgcgtcatca tctccttggc cgccttgttg
     1381 gtgaaggtca ccgccatcac gccgcccggc gagacctgtc cggtggacag cagccaggcg
     1441 atgcgggtcg tcagcacccg ggtcttgccc gagcccgcgc cggcgagaat cagcgcatgc
     1501 tcgttcggca aggtgacggc ggcgcgctgc tcgggattga gattctgtaa cagcggggac
     1561 gccgcgtcgg cttctgggaa catacggcat tgtagaaagc ggccgagatc ccactcatga
     1621 acaatctgtc acgcctgatc gtgcccacgc tcctgctggc gctgcatgcg ggcgggctgc
     1681 gggcgcagcc ctcctgctcc agcgacggcc agccgcagcc ggtgggcttg ctggagcgct
     1741 tcatcaatgc cgactgtgaa tcctgctgga gcgatcccgg taccgcccgg cccgcccgcg
     1801 gagaactcgc cctggactgg atcgtgcccg gcggccgcgg ggacgacgcg cccctttccg
     1861 ccgcggcgag ccgtgacgcg ctggcccgcc tggaagcgct gcgtcgagcg gcaccgcctc
     1921 gtgccgccag ctcccgccag aaggtgcaac gtcccgctcg ccggctgcga gtggcgcagg
     1981 gcccgccgat caacggctac atcggcgcct caattgaact gcggcgcggt ggcgagggcc
     2041 cctggaaagc ctggctgctg ctggtggaaa ccattcccgc aggcagcgaa ggcacgcccg
     2101 ttgcgcgcaa cctggtgcgc aatgcgctgc agccggcctg ggatggaaaa aaggcgctat
     2161 cgaaagaaga gcagagacgg ctcttcgaag cgcgtccgat gaatatcccc gagggcgccg
     2221 accccgcccg cctgcgggtg gtgggctggg tggaggatgc acacgggcga atcagggcca
     2281 tcgcgcaatc ccggtgtgag ccgcccggcg ccaaggggta aaatcaagca ccgggcccaa
     2341 gcattttgcg gcccggtttg cttttgcttc cgcggcgaag ctccgggccc gatccaagcg
     2401 ctcatctgtt cgacaaacag ctttctggag cttggcatgg aaattttcga ttacgacaaa
     2461 gtcttgctgc tgccgcgcaa atgccgcgtg gaaagccgat ccgaatgcga cgccggcgtg
     2521 gagctgggcg gccgcagttt tcgcatcccg gtggtaccgg ccaacatgaa aacggtggtg
     2581 gacgaaaagc tctgcctgtg gctggtgcaa aacggctact tctatgtgat gcaccggttc
     2641 gacttcgaca acgtggtgtt catgcgcaag atgaaggccc agggcgcgta cgggtcgatt
     2701 tcggtgggcg tcaagagcgc cgaccacacg atggtggacc agctggcggc cgagggcctg
     2761 gtgcccgagt acgtcactat cgatgtcgcg cacggccacg ccgacacggt gaaaaccatg
     2821 atcgggcacc tcaaggacaa gctgcccacc gcgttcgtca tcgcggggaa cgtggccacc
     2881 cccgaggcgg tgatcgacct ggagaactgg ggcgccgacg cgaccaaggt gggcgtgggc
     2941 cccggcaagg tctgcatcac gcgcatgaag accggcttcg gcaccggtgg ctggcagctg
     3001 tcggcgctga agtggtgtgc ccgggtggcg accaagccga tcatcgccga cggcggtatc
     3061 caggagcacg gcgacatcgc caagagcgtt cgcttcggcg cgaccatggt gatgatcggc
     3121 gcgctgctgg cgggacacga ggaatctccc gggcagaccg tggacgtgga cggcaagctc
     3181 tacaaggagt acttcggcag cgcgtcggac ttcaacaagg gcgagtaccg gcacgtggaa
     3241 ggcaagcgca tcctcgagcc ggtcaagggc aaactggcgg acacgttgcg ggaaatggaa
     3301 gaagacgtcc agagttcgat cagctacgcg ggcggcaaga agctgatgga catccgcaag
     3361 gtcaattacg tgatcctggg cggcgacaac gcgggcgaac atctgttgat gtagcggccg
     3421 cgctatctgt catgccggac aagatccggc atccatgact tcctgggtca agcccgcaat
     3481 gacagaggga tttgtcatcc cggactcgat ccgggatcca tgacttcctg ggtcaagccc
     3541 gcaatgacag agggatttgt catcccgggc ttgacccggg atccatgaat tcctggattg
     3601 cgggtcgagc ccgcaatgac atcaatagcg ccgtagcaat tgcagcgcca gctggtgcag
     3661 gcgctgcccc ggctccacca gcgcgtcgag cacactgaag tgattcaggc cgggcaaggc
     3721 ttcgcagacc ggcacccgct cttcacccca ggcctcgcga atcaggcggt tctggcgctg
     3781 gaattcctcg ctttcgtcgc cccccgtcac cgtatggagc acgccgtggt ccggcgccgg
     3841 caaccgtgcc ggactcgcct ggcgaacctg ctcgtgcgtg agctgaaggg aaggctggag
     3901 aaaaggcgtg tgcatgatcg gctcgagatc gtgcaggccg gaaatcgata gcgctccctt
     3961 gacgaggtcg gctggaaggt cgcggtcgaa cgccggccac aggcatgcga gcatcatcgc
     4021 cgccagatgg ccgccggccg agtgcccggc gacggtgatt ctgtgcgggt cgcctccgtg
     4081 ccgtgcgatg ttgcgccagg tccaggccag ggcctgcacc agctgcatcg tgatctgcgg
     4141 aatggtcacc gccgggcaca gcgcgtagtt gggaaggacc acgcacgcac cctcgcgcgt
     4201 gaacgcgggc gccacgaagg aatgatcgct cttgtccagc gcccgccagt agccgccgtg
     4261 gatgaagacc agcaccggcg cgcccgcctg cgccgccggg aagatgtcca gggcctcgcc
     4321 cggtcccccg ccatagcgca catccagttc gcgcgtttgg gtatgcaccg cctcggccga
     4381 agcgtcggcc cagcgactca aatgttcggg gtgcgcgggg accaattcgc ggttgttgta
     4441 catgcggtcc agccattggc cgtcgtagcg ggtgctcatg tttcgtattc cctggtcgaa
     4501 ggcatcatct tgtcacacca cgaacgtcgc tgggcttgcg gccgcggccc ggcggctgta
     4561 ttctgaatac agagtacagt caaccatgtc cgcccagctc gtcaggatag caaccgctcc
     4621 tgacctcgtc gatcaggtct atcgcagcct gctcgatgcg gtcagcgatg gctcgctggc
     4681 ccccggcgag cgcattactc aggaagacat cgccgccagg ctggcggtgt cgcgccagcc
     4741 cgtgctgcag gcgctgcgaa tgctcaagaa agacggcttc ctcgccgacg cgccgggccg
     4801 gggcctgatg gtgacgccgc tggacccgga ctggatactc aaggtttacc aggtgcgcgg
     4861 tgcgctcgac gccctcgcgg cgcggctggc ggcgtcacgg cgcgtccggc tcgatgcgaa
     4921 gctcatcgag cacggccgca aagccgcgcg cggcaaggac gtcaaagcca tgatcgacgc
     4981 cgacatggcg ttccacaacg ccatctacgc ggcgtcgcgc aacccgttga tcgagcagag
     5041 cgcgcaccag cattggcggc acctgcgccg ggtgatgggc gcggtgctgc agcagtccag
     5101 gcaacgcgaa acggtgtggg atgaacacga agcgatcgcc agggcgattg cggccggcga
     5161 cgccgacaag gccgcgcgcc tcattgaaca gcacagccag cacgccagca aggacctggc
     5221 cgcgcggctg acccatgtat tgaagaccag tgcatgagcc cgctttccac acagtaaagg
     5281 agaccatgaa catgagactg tcgaaagatc aactcgaaga gttcgaccgc gaaggctacc
     5341 tgttcttccc cggccgggtc acggccgcgg aaatgaagtc gctaaccgac gctgtgcccg
     5401 agctgtacag ccggcgcgag g
//
LOCUS       DASWIY010000393         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1016479, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000393 DASWIY010000000
VERSION     DASWIY010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016479"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..1338)
                     /locus_tag="VGA59_13905"
     CDS             complement(337..1338)
                     /locus_tag="VGA59_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720816.1"
                     /translation="MALTNPGDHVPSVRIYERDTDALFLRVLRNRLDLPTLLAQLVTG
                     KQLTSTTEVKGQVRHAVGTGSIDIVVRFRGGPVLLIENKIDAAYSITRDGHGQPQRYR
                     RSVTAFREAGTEAFSVLLAPRAYLLSSRLADLFDARISYEELRDFVEDQDRVLLDMAI
                     LQAEAPYEPVVNAHASEFFAAIRELIIRRFPDLVMKHDPNDSGVRPDDSRTVYFDVPR
                     TLRLYGGVPRPRMSLQCRDSGAPSASVKIMLGNGAAQADRLSAPQSLRDIGGYLRPAG
                     RSLGIVVDTPRLDTQRPFEEQADDVTEALEAAVRLQGWWNENGETLQQWAKAFTRCR"
     gene            complement(1476..1551)
                     /locus_tag="VGA59_13910"
     tRNA            complement(1476..1551)
                     /locus_tag="VGA59_13910"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1516..1518),aa:Ile,seq:cat)
     gene            1735..2031
                     /locus_tag="VGA59_13915"
     CDS             1735..2031
                     /locus_tag="VGA59_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720817.1"
                     /translation="MLQQDFVAFLILAVIAVVVSGVLHYVCKYYVTDDVWSFLSKCVV
                     GWFGAWLGSPVLGHWFAGLNYGSVYYVPAAIGALGAVIVAVDMARMFAGRGGGS"
     gene            complement(2045..>2544)
                     /locus_tag="VGA59_13920"
     CDS             complement(2045..>2544)
                     /locus_tag="VGA59_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720818.1"
                     /translation="LTAWAREAFGGPVAVGGVSLGALTAQMAVSAARHWPAAMRPDAA
                     LLVTTSGAMMPVAMESSLTRAIAMPQRMAEAGWDRERLVEWMPLLEPVGAPAVAPGRI
                     VALLGTEDDVTPYAEGRRLMERWGVPADNVFLRPLGHFSAALGLYRDIGPLRRLAALL
                     GAPLA"
BASE COUNT          426 a          845 c          836 g          437 t
ORIGIN      
        1 gccgccctcg gtcaggatct ccacggtgag catcctcatc tgcgtgctgg ccctggggct
       61 gacgctcgtc gcccagaggt tcgcctccag gctctcggtg ctgccgcgcg actagggtcc
      121 cccgcccgcg gcccgccggc gggcatcggc ctgcccgggc ttcgcccggc gcggccgggg
      181 cagcggggcc gggccgcttg ccggccattc cccgaggatg gtggaccgtt caggacaaaa
      241 ccgggcactg gtcgataacg atcttgctgc atccgcctca tatcagggag cgttcgactc
      301 taacaatcgt gcctgactag cttctgcgtg gctctttcag cgacagcgag tgaaagcctt
      361 cgcccattgt tggagggttt cgccgttttc gttccaccag ccctggagtc gcacagcagc
      421 ttccagcgct tcggtaacgt cgtccgcctg ctcctcgaac ggcctttgcg tgtccaggcg
      481 cggcgtgtcg accacaatac cgagcgaacg gccggcaggt cttagatagc ccccgatatc
      541 ccggagactt tgtggagcgc tcagccgatc tgcttgtgcg gcgccattgc cgagcataat
      601 cttgaccgaa gcggaaggag ccccgctgtc cctgcattga agcgacattc gtggtcgtgg
      661 aacgccgccg tagaggcgca gtgtgcgtgg aacatcaaag tagacggtgc gagagtcgtc
      721 tggccgcacg ccactgtcgt tcggatcgtg cttcatcacc agatccggaa agcggcggat
      781 aattagttca cgaatagccg caaaaaactc gcttgcatga gcatttacga ccggctcata
      841 aggtgcctca gcctgcaaaa ttgccatatc gagtaatacg cgatcctggt cttccacgaa
      901 atcccgcagt tcctcatagg agatgcgtgc gtcgaagaga tcagcaagtc gactggatag
      961 gagataggcc ctcggtgcaa gcagcacgga gaacgcttca gtgcctgcct cccgaaatgc
     1021 cgtcacactt cttcgatatc gttgcggctg gccatgacca tcacgggtga tgctataggc
     1081 ggcgtcgatc ttgttctcga tcagaagaac tggaccacca cgaaatctaa cgacgatatc
     1141 gatcgagcca gtgccgacgg cgtgccgcac ttgccccttc acctctgtag tgctggtcaa
     1201 ctgctttccg gtcaccaact gagcgagcag cgttggcaga tcaagccgat tgcgcagaac
     1261 gcgcaagaac agggcatctg tgtcccgctc gtaaatacgg actgaaggca cgtgatctcc
     1321 cgggtttgtc agtgccaatg atagcacgaa cctcaccagc taccggttcg cgagcgcccg
     1381 tgcccgatga gccgaggcga ttcaggccgc ggagagtgcc catttttatc tgcacatgga
     1441 tttgctaagc cactgaagcc aaaagcgatt ggcgatggtg ggcccggcag gattcgaacc
     1501 tgcgacaacg ccgttatgag cggcgcgttc tgaccgctga actacgggcc cgcggccaag
     1561 gtatccggtt ctccggcccg gtcaaggccg gccgccggcc ccgcccccgt ccccgccccc
     1621 ggccccggcg gcggcgggcg ccgggtgggc cgaggcgccc cgattgaacg ccccggcgat
     1681 ccgtggcaag gttcgccggt ccccagggcg gtcaaacctc caagggaggg ggacatgctc
     1741 cagcaagact tcgtggcgtt cctgatcctc gccgtgatcg cggtggtggt ctcgggcgtc
     1801 ctccactacg tctgcaagta ctacgtgacc gacgacgtgt ggtcgttcct gtccaaatgc
     1861 gtggtcggct ggttcggcgc gtggctgggc agcccggtgc tgggccactg gttcgcgggg
     1921 ctcaactacg ggagcgtcta ctacgtgccg gcggcgatcg gcgccctcgg cgcggtcatc
     1981 gtggcggtgg acatggcgcg catgttcgcc ggccgcggcg gcggaagctg accgccggcc
     2041 ccgatcaggc caggggcgcg cccaggagcg cggccaggcg ccggagcggg ccgatgtcgc
     2101 ggtagaggcc gagcgcggcc gagaaatggc cgagcggcct gaggaacacg ttgtccgcgg
     2161 gcactcccca ccgctccatc aggcgccggc cctcggcata gggggtgacg tcgtcctcgg
     2221 tgccgaggag ggcgacgatg cgcccgggcg ccaccgccgg cgcgcccacc ggctccaaga
     2281 gcggcatcca ctccacgagg cgctcgcggt cccatcccgc ctcggccatg cgctggggca
     2341 tggcgatggc gcgggtgagg ctgctctcca tggccaccgg catcatggcg ccgctggtgg
     2401 tgaccagcag cgccgcgtcg ggccgcatgg cggccggcca gtggcgggcg gcggacaccg
     2461 ccatctgggc ggtcagcgcc cccaggctga cgccgcccac cgccaccggc ccgccgaagg
     2521 cctcccgcgc ccaggcggtg agga
//
LOCUS       DASWIY010000394         1685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3234460, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000394 DASWIY010000000
VERSION     DASWIY010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3234460"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1215)
                     /locus_tag="VGA59_13925"
     CDS             complement(19..1215)
                     /locus_tag="VGA59_13925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9720819.1"
                     /translation="MSAIDMSVAPARTNIIVLTYAVALLHGLTLVSFPAVSAVLTGEL
                     GLSNSQYGAIFLPQVGFAVLGSLFGGFLTQRLGLKALLLAALLACAASQAALVTTAFV
                     APDARFITVLAGTAMLGLGFGLGGAPLNALPGLLFPSRRDSALVALHTLLGLGLALGP
                     LVAAGFVRAGAWTGFPQSLLVLCSILFLLISISAFPAREPASASSGGETPLRTPGFWG
                     LVGIAVLYAFAEGTFSNWIVIFLTEAKRLPMTTGTAALSAFWAALVAGRLVVSLLALR
                     LPPQPIWRALPLLMIAAFLALPYADSAVSGIALFAAAGLACSAFFPLTIAVASQRFPT
                     QVPWVSSMMIAALMVGVGLGSFATGALRELLPLEQLYRLSIVYPVSALVLILLLDGRR
                     VEVNRR"
     gene            complement(1212..>1685)
                     /locus_tag="VGA59_13930"
     CDS             complement(1212..>1685)
                     /locus_tag="VGA59_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9720820.1"
                     /translation="GRVKVEADLTVPGLPQVFVVGDTAASAAWNGNPVPGLAPAAKQG
                     GRYVARVIRARATGQRPPGPFVYRHLGSLATIGRKSAVADFGRLRLSGGIAWWLWGLI
                     HMYFLVGVRNRVSVMLDWFWAYLTYRPSTRLITGAESGRRPPIPAQQIDKAVTAP"
BASE COUNT          359 a          541 c          576 g          209 t
ORIGIN      
        1 caggctcgaa ccgcatgttc agcggcgatt gacttctacc cggcgaccat caagcagcaa
       61 tatcagcacg agtgccgaaa ccggatatac gatggaaagc cggtacagct gctcgagcgg
      121 caacagttcg cgcaacgcgc ctgtcgcgaa agaccccaga ccaacgccta ccatcagcgc
      181 ggcgatcatc atcgatgaca cccacgggac ttgcgttgga aaacgctgtg acgcgacggc
      241 gatcgtgagc ggaaagaacg cggagcaggc gaggcccgcg gccgcgaaca gggcgatccc
      301 cgagacggcg ctgtcggcgt agggcagggc gagaaaagcg gcgatcatca gcagcggcag
      361 cgcgcgccat atcggttgcg gcggcaggcg cagcgccagc agcgatacca cgagacgccc
      421 ggcgacgagc gcggcccaga aggccgacag cgccgcggtg ccggtggtca tgggcagccg
      481 cttggcctcg gtgagaaaga tcacgatcca gttgctgaac gtgccctcgg cgaaggcata
      541 cagcacggcg atccccacca ggccccagaa accgggggta cgcaacggcg tttctccccc
      601 ggacgacgcg gaggcgggtt ctctcgccgg aaacgcgctg atacttatta aaaggaacag
      661 gatactgcac agcaccagca ggctttgcgg aaatccggtc catgctccgg ccctgacgaa
      721 gcccgcggcg accagcggac cgagcgccag acccaggccg agcagggtat gcagcgccac
      781 cagggcgctg tcgcggcgcg aaggaaacag cagacccggc agggcattga gcggagcacc
      841 acccagtccg aagcccagtc cgagcatggc ggtgccggcg agcacggtga tgaagcgggc
      901 atcgggagcg acgaaggcgg tcgtcaccag cgcggcctgc gaggcggcgc aggccagcag
      961 cgccgcgagc agcagggcct tgagtccgag gcgttgcgtg agaaatccgc cgaacagaga
     1021 accgagcacg gcaaaaccca cctgcggaag aaaaatggcg ccgtactggc tgttgctgag
     1081 gccgagctcg ccggtcagga ccgcgctcac ggcagggaag ctgaccagtg tcaggccatg
     1141 cagcagcgcc acggcgtagg tcagcacgat gatgttcgtg cgcgcaggtg caacggacat
     1201 gtcgatcgcg ctcatggcgc tgtcaccgcc ttgtcgattt gctgcgccgg tatcggagga
     1261 cgtctcccgg attcagcgcc agtgatgagg cgcgtgctcg ggcggtaagt caggtaggcc
     1321 cagaaccagt cgagcatgac cgacacgcgg ttgcgcacgc cgaccagaaa gtacatgtgt
     1381 atcaatcccc acagccacca ggcaatgccg ccggacaggc gcagccggcc gaaatcggcg
     1441 accgcggatt tgcgcccgat ggtagcgagg ctgccaagat ggcgatacac gaaaggcccg
     1501 ggaggccgct gcccggtcgc gcgcgcccgg atgacgcggg cgacataacg tcctccctgc
     1561 ttggccgccg gcgccagtcc cggcacggga ttgccgttcc aagctgcgga ggccgcggta
     1621 tcgccgacga caaacacttg cggcaggccg gggacggtga gatcagcttc gaccttgacc
     1681 cgtcc
//
LOCUS       DASWIY010000395         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_640766, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000395 DASWIY010000000
VERSION     DASWIY010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640766"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1245
                     /gene="tssF"
                     /locus_tag="VGA59_13935"
     CDS             34..1245
                     /gene="tssF"
                     /locus_tag="VGA59_13935"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEX9720821.1"
                     /translation="MPIHELIPFQADALAPEGFATEQAMLPLDSRAFDGYRLLHEYFA
                     FPERYRFFSINGLQRALPLIAGTRFEVVVLLKAGNVALEAVVDANNLLLHCAPVVNLF
                     PRTSDRIPVSANRFEYHAVVDRGRPLDFEIHSITRVTGHHSDSDREVVFQPFYAAADT
                     DRRGSGAYFSVRREPRLLSEKSRRHGPRTGYIGSECFLSLVDANEAPYSAELRQVTVE
                     ALCTNRDLSLLAPTGNAGSDLTLQASAPVESIRFVAGPTRPTPALAEREITWRLISHL
                     SLNYLTLTDDDPEQGAAALRELLGLYARLGAADAEAQIGAIQKLSVTEVNRRVPGRGP
                     IVFGRGVQLDLLVDEIPLAGVSPWLLGAVLEQFFARHVGLNSFTEFALRSTQRGQVAR
                     WHPRIGRRPSV"
     gene            1242..>1589
                     /locus_tag="VGA59_13940"
     CDS             1242..>1589
                     /locus_tag="VGA59_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEX9720822.1"
                     /translation="MTKQPNATAATPPLMRLGKLFAGLEADATQFDFYYALRHIDAAI
                     AGAVPLGRAARPHDEPLRLTQHPSLAFAPAALHQYKPGVAAHGLGRLSVHHFGLFGPN
                     GALPQHLTEYVQER"
BASE COUNT          252 a          542 c          482 g          313 t
ORIGIN      
        1 cagtgcctgg gcctgttggt ccacgccggc cgcttgccga tccatgaatt gattcctttc
       61 caggccgatg ccctcgcacc cgagggcttt gcgaccgaac aggccatgct gccgctggat
      121 tcacgcgctt tcgacggcta ccgcctgctg catgagtact ttgcatttcc ggagcgctac
      181 cgtttctttt ccatcaacgg tttgcaacgc gccttgccgc tgatagcggg cacccggttc
      241 gaagtcgtcg tgctgctcaa ggccggcaac gtcgcgctcg aggcagtagt cgacgcgaac
      301 aatctgttgc tgcactgcgc gccggtcgtc aacctgtttc cccgtacatc ggaccgcata
      361 cccgtcagtg ccaaccggtt cgaataccac gcggtggtcg atcgcggccg gccgctggat
      421 ttcgagatcc acagcatcac ccgcgtcacc gggcatcaca gcgactccga ccgcgaagtc
      481 gtcttccagc cgttttacgc ggccgccgat accgacaggc gcggttccgg ggcctacttc
      541 tcggtcaggc gcgagcctcg gctcctgtcc gagaaatccc gccgccatgg accgcggacc
      601 ggctacatcg gcagcgagtg cttcctgtcg ctggtcgatg ccaacgaagc accctacagc
      661 gccgaactga ggcaggttac cgtcgaggcc ttgtgcacca accgggatct gtcgctgctc
      721 gcgcccaccg gcaacgcggg ctcggatctc accttgcagg cttccgcgcc cgtcgaaagc
      781 atacggtttg tggccgggcc cacccggccg acacctgcgc tggcggagcg ggagatcacc
      841 tggcgtctga tttcccacct cagtctcaac tacctgacgc tgaccgacga cgacccggag
      901 cagggcgcgg ctgccttgcg cgagctgctg ggcctgtatg cccgtctggg cgcggcagac
      961 gctgaagccc agatcggcgc gatccagaag ttgtccgtga ccgaggtcaa ccgtcgtgtg
     1021 ccggggcgtg gacccatagt tttcggtcgt ggcgttcagc tcgatctgct ggtcgatgag
     1081 atcccgctgg cgggtgtcag cccatggctg cttggcgccg tgctggagca gttttttgcc
     1141 aggcatgtgg gcttgaactc atttaccgaa tttgccttgc gcagtactca gcgcggtcaa
     1201 gttgctcgtt ggcacccgcg cattggacgg cgcccatcgg tgtgacaaaa cagcccaacg
     1261 caaccgcggc aacaccgccg ctgatgcggt tgggtaagct gtttgcgggc ctggaagccg
     1321 atgcgaccca gttcgacttc tactacgccc tgcgtcacat cgatgctgct atcgcaggtg
     1381 ccgtgcccct ggggcgcgct gcgcgaccgc acgatgaacc cctgcgcctg acccagcacc
     1441 catcgctggc gttcgctccg gctgcgctgc atcagtacaa acccggcgtt gcagcccatg
     1501 gactgggccg gctttccgtg catcatttcg ggctcttcgg ccccaacggg gcgctgcccc
     1561 agcatctgac ggagtacgtt caagagcgc
//
LOCUS       DASWIY010000396        12152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1020610, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000396 DASWIY010000000
VERSION     DASWIY010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 12152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12152
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020610"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VGA59_13945"
     CDS             complement(<1..520)
                     /locus_tag="VGA59_13945"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9720823.1"
                     /translation="MLFGRTQRKPISIPAKEVKEWKYTTCNYCSTGCAIEIGLNDKGK
                     IVTSRGHAGADVNRGKLCIKGLLEHELFDSPGRGTVPLMREKAHLPFQPAGWDAALDK
                     TAEKIKEIQARYGRDSFAVVSTGQLLTEEFYTLGKLVRGQIGTNNYDGNTTLCMASAV
                     SGYKRSFGSDGPP"
     gene            complement(521..907)
                     /gene="nirD"
                     /locus_tag="VGA59_13950"
     CDS             complement(521..907)
                     /gene="nirD"
                     /locus_tag="VGA59_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEX9720824.1"
                     /translation="MNEWKKICLVTDIPVLGSRCVKRPQGMDVAVFRNDRDQVFALLD
                     RCPHKGGPLSQGIVFGTSVACPLHNWTIGLNDGRARQPDEGCAQKFSVKVEGGAVYLN
                     ALELAGLATDAAKPVAGPKRRETVGG"
     gene            complement(978..3455)
                     /gene="nirB"
                     /locus_tag="VGA59_13955"
     CDS             complement(978..3455)
                     /gene="nirB"
                     /locus_tag="VGA59_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEX9720825.1"
                     /translation="MKRSKLVMVGNGMAGVRTIEELLKVAPELYDIAVFGAEPHPNYN
                     RILLSPVLAGEQSLDEIVLNSWEWYQENNIRLHAGKKVVEVDRVRRVVRADDGTEEEY
                     DRLLMCTGSKPFILPVPGNQLAGVIAYRDIADTNAMIEASQKYKKAVVIGGGLLGLEA
                     ANGLMRRGMDVTVVHVMPWLMERQLDDVAGKLLQKSLEDRGLKFMIGAQTQELISDKD
                     GRVMAIKFKPVPGCDPGDGTEVPTNLVVMAVGIRPNTELAEKARLHCNRGIVVNDTMQ
                     TVTDARIYSVGECAAHRGIAYGLVAPLFEQAKVAANHLAQFGIGRYSGSLTSTKLKVT
                     GIDLFSCGDFMGESSKRPEDAGTEQIVMSDPSRGVYKKLVIKDGKLVGACLYGDTVDG
                     SWYFKLLRDGRSVSDIRDKLMFGESSIGDSGHEGHTRAATMPDDAEVCGCNGVNKGTI
                     CKAIKEKGLFTLDEVKKHTKASASCGSCTGLVEQILMFTAGGDYSATPKLKPMCACTD
                     HGHQAVRDAIRDNKLLKIADVFAFMEWKTPNGCASCRPAVNYYLISTWPKEAKDDPQS
                     RYINERSHANIQKDGTYSVIPRMWGGETTADELRRIADAVDKYQIPTVKVTGGQRIDL
                     LGVRKEDLQNVWKDIGMPSGHAYAKALRTVKTCVGSEWCRMGTQDSTQMGKDLERAMW
                     RMYAPHKVKFAVSGCPRNCAEAGIKDVGIIGVDSGWEMYIAGNGGIKTEVAQFFTKLR
                     TSEEVLEYTGAFMQLYREEGWYLERTVHFVGRVGLDYVKKRILGDHDGRKALWARLQA
                     ALDGEPDPWFEFDKAAVDTRQFAPLSV"
     gene            3608..5341
                     /locus_tag="VGA59_13960"
     CDS             3608..5341
                     /locus_tag="VGA59_13960"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HEX9720826.1"
                     /translation="MAFEIDIGFTSLPGRKELNEDFAAAMLPEPGQEAMGAIAAIADG
                     VSMGGLGREAAQTTVTSLVRDYFGTPETWDTTVALDRVIGAQNAWLAGQNRRRHPACG
                     LTTLTALVVRGQSYALAHVGDTRAYLLRAGTVTQLTHDHAVEHPDFSHKLLRAVGAED
                     RLVVDYRQGDLHVGDVFVLLSDGVHNLVPAGRLQALTIDSAQALSEALVRAALQQGSR
                     DNATAMVVRVLGILESTLHDENRLAQQLPISPRLKVGDTIDALTVTALVSDNGINILY
                     QVRDPATRKLYALKTLDPRRAHDAQERGMLAHEAWLAKRMRSGRAAENLVGVVDPLVD
                     SPSAFYLLYEWHGGETLQQMLDRKQKFALNQVVAVATQAARALGRLHRQGVIHRDVKP
                     ANLHQGEDGVLRLLDLGVALTGREPEAARLLHAGTPSYMNPEQWGFHAQAGQGPEEPP
                     DAQSDLFALGVTIYQLLTGGKLPYGDVLPYQAGRYFRDPIAPSRRNPGVPIWLDHVVL
                     KAVARDKRQRFETAEEMVLALERGASRPLTAPPPTPLMQRDPTALWKIALAVSVLFNA
                     LLVYWLLFLPK"
     gene            complement(5348..6628)
                     /locus_tag="VGA59_13965"
     CDS             complement(5348..6628)
                     /locus_tag="VGA59_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9720827.1"
                     /translation="MAGFRTFLKSGHAPTLFAAFLYFDFCFAVWVLNGAMAPFISEAY
                     QLTAAQKGFMISVPILAGALMRFPLGILAQYIGRKNAALVEMGLILLALAYGYFFISS
                     YDHVLAMGVLLGIAGASFGVALSLGSGWFPPKYKGLAMGIAGAGNSGTVLAVLFAPPL
                     AMAYGWQAVYGLAAFFMLIPMVVMVVFAKEPPDIEHQTFRQHIACLFEKDGWAFSLIY
                     VVTFGGFIGLASFLPTYFHDQFGVTKVQAGQLTMLSALMGSAVRVVGGYISDRAGGIN
                     TLTGVLAIVAVTLVLCGAAGHSLTLTTLLFMVCFAALGAGNGALFQLVPLRWPLATAV
                     AGSMIGEVGALGGGFLPNAMGLSKEYSGTYFWGFLAFALLAIGLLVMLRLMQIRWTRT
                     WAEKGGRARPNAPAPAPLPEAKPAAAKLTTRSRR"
     gene            complement(6799..7260)
                     /locus_tag="VGA59_13970"
     CDS             complement(6799..7260)
                     /locus_tag="VGA59_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HEX9720828.1"
                     /translation="MTNALHDTELKLFVDSVRRYFQVTTRQEPQITSAFLGTGDIDGH
                     DFNGIVSFSGSYNGHVIVSMTAQLLRELLLLQGETDLSTGNLLDAAGEIANTLGGNAR
                     KMLGSGLQISVPVKLHGASGVKARVRKHPYVITLRWNHQPAMVCVDMERRH"
     gene            complement(7257..7628)
                     /locus_tag="VGA59_13975"
     CDS             complement(7257..7628)
                     /locus_tag="VGA59_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9720829.1"
                     /translation="MRLLIVDDSNMIRSRISRVVQNGGLANIALVGLARNGAEAVRIA
                     RSTRPEVVTMDLTMPEMDGVQCIGELLRILPGINILVVSALSDKSTVIAALKLGARGF
                     VAKPFSDDELKLALLDLMEAR"
     gene            complement(7643..9781)
                     /locus_tag="VGA59_13980"
     CDS             complement(7643..9781)
                     /locus_tag="VGA59_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HEX9720830.1"
                     /translation="MAAVLPLESGATGAAAPRGTTGARKARDQRGFSVGKYREIIIAV
                     AFFLLFDLGVLILNFYTSFQIAEDALGVNLSGRQRMLSQRTAKALLSVDVAHAKGLPA
                     DKETEELKGAVELFDVSLKAFRSGATVPGGDGQPVFLHAAEGAKATEILKEAQDIWAP
                     YHDKLAPVLGGTATDSQLQAAVDYAKANNLKLLGLMNELTTALESVASARARNLRAVQ
                     TGGIVLALLNFVFILFKFLRRLQTSDAAIEAATEENREILTSVREGLFLITPDFRLGS
                     QLSRSAHTLFGRQLAPGDDFFALLKPLVSDKVLTDARGYVELLFAPHVKEALVQGINP
                     LSEVGVAVKNRLGQDAQRHLSFHFNRVQEDGAVRHLLVTVQDISARIELEDKLQSERR
                     RSQKEFSMLLKAIDADPALLRQFVAHAETSLLEINDIMRSASAAQGEGAILKRLEEIA
                     RRIHSLKGNAGTLGLESVATQAHLFEAELLRIRESGGASGDLGAALLSLPLPLEDLLG
                     KVAALRSLTGLQRPRQADSGSPDPVNAALARLAQDVAADSGKKVETLVRMGSQSDLEE
                     STGTLVREIAVQLLRNAVVHGIESPMLRKAANKPDQGKVEVKLVRSEAQWTLSVRDDG
                     CGLSAVRVRQKLLDLRWYTAQQLDSFDDRQIVSHIFKPGFSTAGGVSMHAGRGVGLDL
                     VQANVQKLGARILLSSTPGQFTEFKIKFAA"
     gene            complement(9975..10607)
                     /locus_tag="VGA59_13985"
     CDS             complement(9975..10607)
                     /locus_tag="VGA59_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX9720831.1"
                     /translation="MTPSQTLRLVVIAPDSLYPDAGDSHAVEQAERSRQLRIALLENG
                     YNVVAVLPTDSFLEERLAQLRPDMIVVDAESEARDALEHVVMATRDARRPIVMFTNDN
                     DTSHVKDAVAAGVSAYIVAGLSPDRIRPILEVAMARFQHEQALRRELADAKSELHDRK
                     TIDRAKGLLMQRRGLSEQQAYDKLRKSAMDRGLKLGEVAQRILDVADLLA"
     gene            10698..11717
                     /gene="tsaD"
                     /locus_tag="VGA59_13990"
     CDS             10698..11717
                     /gene="tsaD"
                     /locus_tag="VGA59_13990"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX9720832.1"
                     /translation="MLILGIESSCDETGVALVESQGRELPALRAHALYSQVDMHQAYG
                     GVVPELASRDHIRRVLPLTEQVLDDSGRTLAELDVVAYTRGPGLAGALLVGAGVACAL
                     GAALDRPVLGVHHLEGHLLSPFLGADPPEFPFVALLVSGGHTQLMQVNGVGNYKLLGE
                     TIDDAAGEAFDKSAKLMGLGYPGGPALARLAAQGDGSAFKLPRPLLHSGDLDFSFAGL
                     KTAVLTQTRKLGPELKARTPDLAASTQAAIVEVLVKKSLAALEATGLARLVVAGGVGA
                     NQQLRAQLNSACAQRGAQVHYPEAHFCTDNGAMIAMAAAMRIQAGVQQASRTYSFDVK
                     PRWPL"
BASE COUNT         1987 a         3974 c         4071 g         2120 t
ORIGIN      
        1 cgggcggccc gtcggagccg aacgagcgct tgtagcccga aacggccgag gccatgcaca
       61 gcgtggtgtt gccgtcgtag ttgttggtgc cgatctggcc gcgcaccagc ttgcccaggg
      121 tgtagaactc ctcggtcagc agctggccgg tggagaccac ggcgaacgag tcgcgcccgt
      181 acctggcctg gatttccttg attttttccg ctgtcttgtc cagcgccgcg tcccagccgg
      241 cgggctggaa cggcaggtgc gccttctcgc gcatcagcgg cacggtgccg cggccgggcg
      301 agtcgaacag ctcgtgctcc agcaggccct tgatacacag cttgccgcgg ttcacgtccg
      361 cgccggcatg accgcggctg gtgacgatct tgcccttgtc gttcaggccg atttcaatgg
      421 cgcagccggt cgagcagtaa ttgcacgtgg tgtatttcca ttccttcacc tccttggcgg
      481 gaatggaaat aggcttgcgc tgcgtgcggc cgaagagcat tcagccaccc accgtttctc
      541 tgcgtttcgg tccggcaacc ggcttcgcgg catccgtggc caggccggcc agctccaggg
      601 cgttcaggta gacggcgccg ccttcgacct tcacgctgaa cttctgcgcg cagccttcgt
      661 ccggctgcct ggcgcggccg tcgttcaggc cgatggtcca gttgtgcagc gggcaggcga
      721 cgctggtgcc gaacacgatg ccctgcgaca gcggcccgcc cttatgcggg cagcgatcca
      781 gcagcgcgaa cacctggtcc cggtcgttgc ggaacaccgc cacgtccatg ccctggggcc
      841 gcttgacgca gcgcgagccg agcaccggaa tgtcggtgac caggcagatc tttttccatt
      901 cgttcattca tttctcctgt catcccgggc ttgacccggg atccatggat tgcgggtcga
      961 gcccgcaatg acaacaacta aacactcaac ggcgcgaatt ggcgcgtgtc caccgccgcc
     1021 ttgtcgaact cgaaccaggg atcgggctcg ccgtccagtg cggcctgcag gcgcgcccac
     1081 agggccttgc ggccatcgtg gtcgccgagg atcctttttt tcacgtagtc caggccgacg
     1141 cggccgacga agtgcaccgt gcgctccagg taccagcctt cctcgcggta cagctgcatg
     1201 aacgcgccgg tgtactcgag gacttcctcg gaagtcctga gcttggtgaa gaactgggcc
     1261 acctcggtct tgatgccgcc gttgccggcg atgtacatct cccagcccga gtccacgccg
     1321 atgattccga cgtccttgat gccggcctcg gcgcagttgc gcgggcagcc ggacacggcg
     1381 aacttgacct tgtgcggggc atacatgcgc cacatggcgc gctccaggtc cttgcccatc
     1441 tgcgtggagt cctgcgtgcc catgcggcac cattcgctgc ccacgcaggt cttcaccgtc
     1501 cgcagcgcct tggcgtaggc gtggccgctg ggcatgccga tgtccttcca gacgttctgg
     1561 aggtcttcct tcctgacgcc cagcaggtcg atgcgctggc cgcccgtcac cttgacggtg
     1621 gggatctggt acttgtccac cgcgtcggca atgcggcgca gctcgtcggc cgtggtttcg
     1681 ccgccccaca tgcgcgggat gacggagtaa gtgccgtcct tctggatgtt ggcgtggctg
     1741 cgctcgttga tgtagcggct ttgcggatcg tccttggcct ctttcggcca ggtggagatc
     1801 aggtagtagt tgaccgcggg gcggcagctg gcgcagccgt tgggcgtctt ccattccatg
     1861 aatgcgaaca cgtcggcgat cttcagcagc ttgttgtcgc gaatggcgtc gcgcactgcc
     1921 tggtggccgt ggtcggtgca agcgcacatg ggcttgagct tgggcgtggc cgaatagtcg
     1981 ccgccggcgg tgaacatcag gatctgttcc accaggcccg tgcaggagcc gcaggaggcg
     2041 ctggccttgg tgtgcttctt cacctcgtcg agcgtgaaca ggcctttttc cttgatggcc
     2101 ttgcagatgg tgcccttgtt cacgccgttg cagccgcaga cctcggcgtc gtcgggcatg
     2161 gtggcggccc tggtatggcc ttcgtggccg ctgtcgccga tgctggattc gccgaacatc
     2221 agcttgtcgc ggatgtcgct gacgctgcga ccgtcgcgca gcagcttgaa ataccagctg
     2281 ccgtccaccg tgtcgccgta caggcaggcg ccgaccagct tgccgtcctt gatgaccagc
     2341 ttcttgtaga caccgcgcga ggggtcggac atgacgatct gttccgtgcc cgcgtcctcg
     2401 ggacgcttcg atgactcgcc catgaagtcg ccgcaggaga acaggtcgat gcccgtgacc
     2461 ttgagcttgg tggaggtgag cgagcccgag tagcggccga tgccgaactg cgccaggtgg
     2521 ttggccgcca ccttggcctg ctcgaacagc ggcgccacca ggccataggc gattccgcgg
     2581 tgggctgcgc attcgccgac ggagtagatg cgcgcgtcgg tcaccgtctg catcgtgtcg
     2641 ttgaccacga tgccgcggtt gcaatgcagg cgcgccttct cggccagttc cgtgttgggg
     2701 cgaatgccga cggccatgac gaccaggttg gtggggactt cggtgccgtc ccccgggtcg
     2761 cagcccggga caggcttgaa cttgatggcc atgacgcgtc catccttgtc cgagatcagc
     2821 tcctgcgtct gcgcgccgat catgaacttc aggccgcggt cttccagcga cttctgcagc
     2881 agcttgcccg ccacgtcgtc cagctggcgc tccatgagcc agggcatgac gtgcaccacg
     2941 gtgacgtcca tgccgcgccg catcaggccg ttggccgctt ccaggcccaa caggccgccg
     3001 ccgatgacca cggccttctt gtacttttgg gaggcctcga tcatcgcgtt ggtatcggca
     3061 atgtcgcggt aagcgatcac gcctgcgagc tgattgcccg gcacgggcag gatgaaaggc
     3121 ttggagccgg tgcacatcag cagccggtcg tattcttctt cggtgccgtc gtcggcccgg
     3181 accacccggc gcacgcggtc gacttcgacc accttcttgc cggcatgaag gcggatgttg
     3241 ttctcctggt accactccca ggaattgagc acgatctcgt ccagcgactg ctcgccggcc
     3301 agcacgggcg acaggagaat acggttgtag ttgggatgcg gctcggcgcc gaacaccgcg
     3361 atgtcgtaaa gctcgggtgc gaccttcaag agctcttcga tggtgcgcac gcccgccatt
     3421 ccgttgccca ccatcaccag cttcgatctt ttcatttcac tctccaatga atactgccgc
     3481 tacgggagtg caagggtcat gccagtttgt tgggggcccg cgctatcggc agcggcgcac
     3541 caaagggtgg cggccggttg atgttgcggc gcaccaagat gggccccgat cttgcttaag
     3601 ctggtgcatg gctttcgaaa tcgacatcgg cttcacctcg cttcccggcc gcaaggagtt
     3661 gaacgaggac ttcgccgccg cgatgctgcc cgagccgggg caggaagcca tgggcgccat
     3721 cgccgcgata gccgatggcg tgagcatggg gggcctcggc cgcgaggcgg cccagaccac
     3781 cgtgaccagc ctggtgcgcg actatttcgg cacgccggag acatgggaca ccacggtggc
     3841 gctggaccgt gtcatcggcg cccagaacgc ctggctcgcg ggccagaacc gccgccgcca
     3901 ccctgcctgt ggactcacga cgctcacggc gctggtcgtg cgcggccaga gctacgccct
     3961 ggcccacgtg ggcgacacgc gcgcctacct gttgcgcgcc gggacggtga cacagctcac
     4021 gcacgaccat gccgtggagc acccggactt cagccacaag ctgctgcggg ccgtcggcgc
     4081 cgaggaccgg ctggtggtgg actaccgcca gggcgacctg cacgtgggcg acgtattcgt
     4141 cctgctgagc gacggcgtac acaacctcgt gccggccggc cgcctgcagg cgctcacgat
     4201 cgacagcgcc caggcattga gcgaagcgct ggtgcgggcg gcgctccagc agggcagccg
     4261 cgacaacgcg acagcgatgg tggtgcgagt gctcggcatc ctggagagca cgctgcacga
     4321 cgagaaccgg ctcgcgcagc aactgccaat ttccccccgg ctgaaggtgg gcgacacgat
     4381 cgacgctctg acggtgaccg cgctcgtgtc cgacaacggc atcaatattc tgtaccaggt
     4441 gcgcgatccg gcaacgcgca agctctacgc cctgaagaca ctcgatcccc ggcgcgcgca
     4501 cgatgcgcag gagcgcggca tgctggcgca cgaggcctgg ctggccaagc gcatgcgctc
     4561 gggccgcgcg gccgaaaacc tggtcggcgt ggtcgatccg ctggtcgact cgcccagcgc
     4621 tttctatctg ctgtatgaat ggcacggcgg cgagacgctg cagcagatgc tcgatcgcaa
     4681 gcagaagttc gcgctgaacc aggtggtcgc ggttgccacc caggcggctc gcgcgctggg
     4741 gcggctgcat cgccagggtg tgatccaccg cgacgtgaag ccggccaacc tgcaccaggg
     4801 cgaggacggc gtgctgcgcc tgctggacct gggcgtggcg ctgaccgggc gcgagccgga
     4861 ggccgcgcgg ctgctgcatg ccggaactcc cagctacatg aatccggagc agtggggttt
     4921 tcacgcgcag gccgggcaag gcccggagga gccacccgac gcgcaaagcg acctgttcgc
     4981 cctgggcgtc acgatctacc agcttctcac cggcggcaag ctgccctacg gcgacgtgct
     5041 gccgtaccag gccgggcgct actttcgcga tccgatcgca ccgagccggc gcaaccccgg
     5101 ggtgccgatc tggctggacc atgtggtgct caaggccgtc gcccgcgaca agcgccagcg
     5161 cttcgagacg gccgaggaaa tggtgctggc gctggagcgc ggtgcgtcgc gcccgctcac
     5221 ggccccgccg cccacgccgc tgatgcagcg cgatcccacg gccctgtgga agatcgcgct
     5281 ggccgtcagc gtgctgttca acgcgctgtt ggtttactgg ctgctgttcc tgcccaagta
     5341 attaccacta gcgccggctg cgcgtcgtca gcttggcagc ggccggtttg gcttcgggca
     5401 ggggcgcggg cgccggcgcg ttgggccggg cccggccgcc tttttcggcc cacgtgcggg
     5461 tccagcggat ctgcatcaag cgcagcatga ccagcaggcc gatggccagc agcgcgaaag
     5521 ccaggaagcc ccagaagtag gtgccggagt attccttgga caagcccatc gcattgggca
     5581 ggaagccgcc gcccagggcg ccgacctcgc cgatcatcga gccggcgacc gcggtcgcca
     5641 gcggccagcg tagtggcacc agctggaaca gcgcgccatt gccggcgccc agcgcggcga
     5701 agcacaccat gaagagcagc gtggtcaggg tgagcgagtg gccggctgcc ccgcacagca
     5761 ccagcgtcac cgccacgatc gccagcaccc ccgtcagggt gttgatgccg ccggcgcggt
     5821 ccgagatgta gccgccgacc acgcgcacgg ccgagcccat cagggccgag agcatggtga
     5881 gctggccggc ctgcaccttg gtcacgccga actggtcgtg gaagtaggtc ggcaggaaac
     5941 tcgccaggcc gatgaagccg ccgaaggtga cgacgtagat caggctgaag gcccagccgt
     6001 ccttctcgaa caggcaggcg atgtgctggc ggaaagtctg gtgttcgatg tccggcggct
     6061 ccttggcgaa gacgaccatc accaccatcg ggatcagcat gaagaacgcc gccagtccgt
     6121 acacggcctg ccagccatag gccatggcca ggggcggcgc gaacagcacc gccagcacgg
     6181 tgcccgagtt gccggcgccg gcgataccca tcgccaggcc tttgtacttg ggcgggaacc
     6241 agccggaacc cagcgagagg gccacgccga agctggcacc ggcgatgccc agcagcacgc
     6301 ccattgccag cacgtggtca taggagctga tgaagaaata gccgtacgcc agagcgagca
     6361 ggatcaggcc catctccacc agcgccgcgt tcttgcgccc tatgtactgg gccaggatcc
     6421 ccaacgggaa acgcatcagc gcgccggcca ggatgggcac ggaaatcatg aagccctttt
     6481 gcgccgcggt cagctggtag gcctcgctga tgaagggcgc catcgcgccg ttcaggaccc
     6541 agaccgcgaa gcagaagtcg aaatacagga acgcggcgaa cagcgtcggt gcgtggcctg
     6601 acttcaagaa ggtgcgaaaa cccgccatgg tgcaaatcct ctgtaaatct gaagggggaa
     6661 gttcacagaa tcagtgcgag aagcgtgcca gcttggggca cgccgaaacc cgcccccctg
     6721 cgtcaaagca gggcgtcgtg caagccttgt tggtgcgctg caccaagttg gccgcgaaag
     6781 tcgcctagcg tggccggtct agtggcgcct ctccatatcg acgcacacca tcgccggctg
     6841 atggttccag cgcaaggtga tgacgtaagg gtgcttgcgc acgcgcgcct tgacgccgga
     6901 cgcgccgtgc agtttcaccg gcaccgaaat ctggaggccg gagcccagca tcttgcgggc
     6961 attgccgccc agcgtattgg cgatctcgcc cgccgcgtcc agcaggttgc ccgtggacag
     7021 gtcggtctcg ccctgcaaca gcagcagctc gcgcagcagc tgcgctgtca tggacacgat
     7081 cacgtggccg ttgtaggaac cggagaagga gacgatgccg ttgaagtcat ggccgtcgat
     7141 gtcgccggtg cccaggaagg cggaggtgat ctggggttcc tgccgggtgg tgacctgaaa
     7201 gtagcgccgc acggagtcga cgaagagctt cagttcggtg tcgtgcaagg cgttggtcat
     7261 ctggcctcca tcaggtccag cagggccagc ttgagttcgt cgtcggaaaa cggcttggcc
     7321 acgaagccgc gcgcgccgag cttgagcgcg gcgatcacgg tggatttgtc ggacagggcg
     7381 ctgaccacca ggatgttgat cccgggcagg atgcgcagca gctcgccgat gcactgcacg
     7441 ccgtccatct ccggcatggt caggtccatg gtgacgacct cgggccgggt cgaacgggcg
     7501 atgcgcaccg cctcggcccc gttgcgcgcc aggccgacca gcgcgatgtt ggcgagcccg
     7561 ccgttctgca ccacgcgcga gatgcgcgag cggatcatgt tggagtcgtc gacgatcagg
     7621 aggcgcatgg gcaacgtccc ggctaggcgg cgaacttgat cttgaactcg gtgaattggc
     7681 cgggcgtgga actcagcagg atacgcgcgc cgagtttctg cacgttcgcc tgcaccaggt
     7741 ccaggccgac gccgcggccg gcatgcatgg acacaccgcc ggccgtggag aagccgggct
     7801 tgaagatgtg agagacgatt tgccggtcgt cgaagctgtc cagctgctgc gccgtgtacc
     7861 agcgcaggtc cagcagcttt tgccgcacgc gcaccgcgga gaggccgcag ccgtcatcgc
     7921 gaaccgagag cgtccactgc gcttcgcttc gcaccaattt cacctcgacc ttgccctggt
     7981 cgggtttatt cgcagccttg cgaagcatgg gcgattcgat gccgtgcacc acggcattgc
     8041 gcagcagctg caccgcgatt tcgcgcacca gcgtgccggt cgattcttcc aggtcgctct
     8101 ggctgcccat gcgcaccagg gtttcgacct tcttgccgct gtcggcggcc acatcctgcg
     8161 ccagcctggc caacgcggca ttcacggggt cgggtgagcc gctgtccgcc tgccgcgggc
     8221 gctgcagccc cgtgaggctc ctgagcgcgg ccaccttgcc cagcaggtct tccaggggca
     8281 gcggcaagga cagcagggct gcgccgagat cgccgcttgc gccgcccgat tcgcggatgc
     8341 gcagcagctc ggcttcgaac aggtgcgcct gcgtggcgac gctttcaagg cccagggtgc
     8401 cggcgtttcc cttgagcgag tggatgcggc gcgcgatttc ctccaggcgc ttgaggatcg
     8461 cgccttcgcc ctgagcggcc gaggcgctgc gcatgatgtc gttgatctcc agcaggctcg
     8521 tctcggcgtg cgccacgaat tggcgcagca gcgccggatc ggcgtcgatc gccttgagca
     8581 gcatcgagaa ttccttttgc gaacgccggc gctcgctttg cagcttgtcc tccagctcga
     8641 tgcgtgccga gatgtcctgc accgtcacca gcaggtggcg cacggcgccg tcctcctgca
     8701 cgcggttgaa gtggaacgac aggtggcgct gcgcgtcctg gccgaggcga ttcttcacgg
     8761 cgaccccgac ctccgacagc ggattgatgc cttgcaccag ggcttccttg acatgcggtg
     8821 cgaacaacag ctccacatag ccccgcgcat cggtcagcac cttgtccgag accagcggct
     8881 tgagcaaggc gaagaagtca tcgcccggcg ccagctggcg gccgaacagc gtgtgcgccg
     8941 accgcgacag ctgcgagccg agccggaaat cgggcgtgat caggaacaag ccctcgcgca
     9001 cgctggtcag gatttcgcgg ttttcctccg tggcggcctc gatggcggcg tccgaggtct
     9061 gcaggcggcg caggaacttg aacagaatga agacgaagtt cagcagcgcc aggacgatgc
     9121 cgccggtctg cacggcgcgc agattcctgg cgcgcgccga agccaccgac tcgagcgcgg
     9181 tggtcagctc attcatcaat cccagcagct tgaggttgtt ggccttggcg tagtcgaccg
     9241 cggcctgcag ctgggaatcg gtcgcggtac cgccgagcac tggcgccagc ttgtcgtgat
     9301 atggcgccca gatgtcctgt gcctctttga ggatttcggt ggccttggcc ccttcggcgg
     9361 catgcaggaa taccggttgg ccgtcgccgc cgggaaccgt cgcgccgctg cggaaagcct
     9421 tgagcgacac gtcgaaaagc tcgacggcgc ctttgagctc ttcggtttcc ttgtcggccg
     9481 gcaacccctt ggcgtgcgcc acgtccaccg acagcagcgc cttggcggtg cgctgcgaca
     9541 gcatgcgctg ccgcccggac aggttcacgc ccagggcatc ctcggcgatc tggaaggatg
     9601 tgtagaagtt caggatcagg acgccaaggt cgaacagcag gaagaacgcc accgcgatga
     9661 tgatctcgcg gtatttgccg acggagaagc cgcgctggtc gcgcgccttg cgcgcgccgg
     9721 tcgtgcctcg gggggcggcg gcgcccgtag cgccggattc caggggcagg actgcggcca
     9781 tgtgaaactc ccgtcgtgga aacaaaaagg catccgcacg caacacggca ccgtgctggt
     9841 ggggacgcct ttgtccatat ggccatgccc tcgcttcgcc gttgaagcgt gccggccagc
     9901 aaaccaacaa tggtttacga gcaaagctat gcagtaacca tgccaggcag gctgaacgcg
     9961 ttcgcgttcg cgtttcaggc cagcaggtca gccacgtcca ggatgcgctg ggccacctcg
    10021 ccgagcttga gcccgcgatc catggcgctc ttacgcagct tgtcataggc ctgttgttcc
    10081 gaaaggcccc ggcgctgcat caagagtccc ttggcccggt cgatggtctt gcggtcgtgg
    10141 agttcggact tcgcatcggc cagctcgcgg cgaagtgcct gttcgtgctg aaagcgcgcc
    10201 atcgccactt ccaggatcgg gcggatgcga tcgggggaca gcccggccac gatgtaagcg
    10261 gagacgccgg ctgcgaccgc gtctttcacg tggctcgtgt cgttgtcgtt ggtgaacatc
    10321 acgatcggcc ggcgtgcatc gcgcgtggcc atcaccacgt gctccagcgc atcacgggcc
    10381 tcgctctcgg cgtcaaccac gatcatgtcg ggccgcaact gggccagccg ctcttccaga
    10441 aagctgtcgg tgggcagcac ggccacgacg ttgtagccat tctccagcag ggcgatgcgc
    10501 aattggcgcg agcgttcggc ttgttccacc gcatgggaat cccccgcgtc cggatagagt
    10561 gaatccggtg cgatgaccac aaggcgcagg gtctgggacg gtgtcatgcc cgaacatggt
    10621 gcaagatttg cgccagaacg ttgcccatcg ggccggcggt gttggggggc ttgccctagg
    10681 gccctaaact cggacggatg ctgatactgg gaattgaatc gtcctgcgac gagacgggcg
    10741 tggccctggt cgagtcccaa ggtcgcgaac tgccggcttt gcgcgcgcac gcgctgtaca
    10801 gccaggtcga catgcaccag gcctatggcg gtgtggttcc ggagctggcc agccgcgacc
    10861 atatccggcg cgtgctgcca ctgaccgaac aggtgctgga cgacagcggg cgaaccctgg
    10921 ccgagctgga cgtggtcgcc tacacccgtg ggcccgggct ggcaggggcg ctgctggtgg
    10981 gcgccggcgt ggcctgcgcc ctgggcgccg cgctcgacag gccggtgctg ggcgtgcacc
    11041 acctcgaagg gcacctgctg tctccattcc ttggcgccga tccgcccgag ttcccgtttg
    11101 tcgccctgct ggtgtccggc ggccacactc aattgatgca ggtgaatggc gtgggcaatt
    11161 acaaattgct gggtgagacg atcgacgacg cagccggcga ggcattcgac aagtccgcca
    11221 agctgatggg cctgggttat ccgggcgggc cggcgctggc gcgcctggcg gcgcagggcg
    11281 atggctcggc tttcaagttg ccgcgtccgc ttttgcacag cggcgatctt gatttttctt
    11341 ttgcaggctt gaagacagcg gtgcttacgc agaccaggaa gctgggcccg gaactcaagg
    11401 cgcgcacgcc ggacctggcc gcatcgaccc aggcggccat tgtggaggtg ctggtgaaga
    11461 aatcgctggc tgcgctggag gcaacgggcc tcgcgcgcct ggtggtggcc ggcggcgtgg
    11521 gcgccaacca gcagctgcgc gcgcagctca atagcgcctg tgcccaacgc ggtgcgcagg
    11581 tccactatcc ggaggcacat ttttgtaccg acaacggcgc gatgattgcc atggccgctg
    11641 cgatgcgcat ccaggccggc gtccagcagg ccagccggac gtattcgttc gacgtcaaac
    11701 cgcgatggcc tttgtgagct gacaacgccc gcgcgcaggc gcgcatctgc tcttcttcca
    11761 tcgcgccgcg acggcgctga acagcacccg ctctttccta gtgcgctgca cgggaaccgt
    11821 gcgcgaatgg ccccaaaaac tgtcatcccg ggctgggctc gcggaacgcc cggggcgcgc
    11881 agctgtccag taaagcgagc ccaatgccgg gccttcgcta tgcacgtacc gaggcatgac
    11941 gctccggccc ggccccgcaa tccatgccac acgtcgcgaa ttgcttggtg ggccaacatg
    12001 gatgccggat cgggtccggc atgacaacgc tttgtcattg ggtcaagccc gggatgcccg
    12061 ggatgcccgg gatgacagtt tttggggcca tccgcgcacg gttcccgtgc agcgcaccag
    12121 gcaagagcgg gtgcgatccg gcgccgccgc gg
//
LOCUS       DASWIY010000397         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3237006, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000397 DASWIY010000000
VERSION     DASWIY010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3237006"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VGA59_13995"
     CDS             complement(<1..305)
                     /locus_tag="VGA59_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9720833.1"
                     /translation="MARPLLHVDRIAKQYTRGWLNKQVTFSLAADFVVEGPTVVGVLG
                     PNGSGKTTLFELITGSNLPSAGRVLVAGQNIHEVRTGERERLAIHYHQSYQVRSFQR"
     gene            complement(305..1036)
                     /locus_tag="VGA59_14000"
     CDS             complement(305..1036)
                     /locus_tag="VGA59_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720834.1"
                     /translation="MSAVLKPMTGTPMLSVERIDTFYGETQVLFEVSLDVGAGEVVAL
                     LGPNGAGKTTTLRSILGLTPARSGAVRFDGRDVTRASTHQIARAGIGWVPDDRRIFPT
                     LTVARNLAIARKRSRFRSWSQKECFEIFSALEYLMHRECENLSGGEMQMVAISRALIG
                     APGLVLFDEPSQGLAPKVVQDVMRTILRLKHEGISVLLVEQNVHSALDVADRVYVMNL
                     GKIVHEGPAERLREDDALRRKLLGV"
     gene            complement(1202..1945)
                     /locus_tag="VGA59_14005"
     CDS             complement(1202..1945)
                     /locus_tag="VGA59_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720835.1"
                     /translation="MALFEAAGLAKRFGDQVVLQDISLSFEEGQLAGIMGPNGAGKTT
                     CFNVLTGLFRPDRGRVTFAGEDITGLTPRAIARKGISRSFQIMNLFNDYSALDNVLVA
                     TPHVRCQGFNPWRDLGADSTAQDLAAMVLARVGLKGKEQAQARSLSYGDRRALEIGVA
                     LAAEPRMLFLDEPTAGLGTEGTARLADLVRELKRQLTIVIIEHDMQFLFKLADAVSVI
                     HWGQVIARGTPAELKQNEWVRRSNLGALR"
     gene            complement(1946..>2744)
                     /locus_tag="VGA59_14010"
     CDS             complement(1946..>2744)
                     /locus_tag="VGA59_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9720836.1"
                     /translation="YAFGLAQKTLWANLWFDLLGAVAMAVILGALVGLFISHRRGIYY
                     ALLTIAFGQVFWFIAIKSHRLTGGEDGLLNIKRLPADFGFAAIDLQSNEALFYFCLAV
                     FALALVALWRLIHSPLGRVLSAIKQNETRAAFVGYNVWLYKWLAFTISAGVAGLAGGL
                     FAMAQQSAYPNVMSLHNSGFVVMMVLIGGGLVSFWGPVIGAAFFILARDLLGAYTETW
                     LLWYGLAFMVMVLFKPEGVAGMWQAWRARRPGQRPAAPVVAVQEREA"
BASE COUNT          480 a          951 c          882 g          431 t
ORIGIN      
        1 ttgcgctgga acgagcgcac ctgatacgac tggtggtagt gaatcgccag ccgctcgcgc
       61 tcgccggtgc gcacttcgtg gatgttctgg ccggccacca ggacccggcc cgcgctgggc
      121 aggttgctgc cggtgatcag ctcgaacagc gtggtcttgc ccgagccgtt cggtcccagc
      181 acgccgacca ctgtcgggcc ttcgacgacg aaatccgcgg ccagcgaaaa cgtcacttgc
      241 ttgttgagcc agccgcgcgt gtactgcttg gcgatgcggt cgacgtgaag aagcggcctg
      301 gccatcacac gcccaacagc ttgcgccgca gcgcatcgtc ctcgcgcaat cgctcggccg
      361 gaccctcgtg cacgatcttg ccgagattca tcacgtagac ccggtccgcc acgtcaagcg
      421 cgctgtgcac gttctgctcc accagcagca cggaaatacc ctcgtgcttg agccgcagga
      481 tggtcctcat cacgtcctga accactttgg gcgccaggcc ctggctgggc tcgtcgaaca
      541 gcaccaggcc gggcgccccg atcagcgcgc gcgagatcgc caccatctgc atctcgccgc
      601 ccgacaggtt ctcgcactcg cggtgcatca ggtattccag cgcgctgaag atctcgaagc
      661 attccttctg ggaccatgag cgaaagcgtg acctcttgcg ggcgatcgcc aggttgcgcg
      721 caacggtcag tgtcgggaag atgcgccggt cgtccggcac ccagccgatg ccggcgcgcg
      781 cgatctggtg cgtgctggcg cgcgtgacat cgcggccgtc gaagcgcact gcgccggacc
      841 gcgccggggt caggcccagg atggagcgca gcgtggtcgt cttgccggcg ccgttgggtc
      901 cgagcagggc taccacttcg ccggcgccca cgtcgagcga cacctcgaac agaacctggg
      961 tttcgccgta gaaggtgtcg atccgctcga ccgacagcat gggcgtgccg gtcatcggtt
     1021 tcagaaccgc gctcatttca gttctccatg gcgctgcacg ccacccgcga cgcatgaaag
     1081 cgcgcggcca aggcgggggc caccgcggaa ccggcttcgc cgggccgctg gtggcgcccc
     1141 ctgaggggga gagggcgaag cccgaggggg catcggccgt aggccgcttc gggggggagc
     1201 ttcatctcag cgcccccagg ttcgagcggc gcacccactc gttttgcttc agctcggccg
     1261 gcgtgccccg cgcgatcacc tggccccagt ggatcaccga caccgcatcg gccagtttga
     1321 agaggaactg catgtcgtgt tcgatgatca cgatcgtcag ttgccgcttg agctcgcgca
     1381 cgaggtcggc cagccgggcc gtaccttccg tgcccaggcc ggccgtcggt tcatcgagga
     1441 agagcatgcg cggctcggcg gccagcgcga ccccgatttc cagcgcccgc cggtcgccat
     1501 acgacaggct cctggcctgc gcctgttcct tgcccttcag gcccacgcgc gcgagcacca
     1561 tggccgcgag atcctgagcc gtgctgtcgg cgcccaggtc gcgccacgga ttgaagccct
     1621 ggcagcgcac atgcggcgtg gccaccagca cgttgtccag cgccgagtag tcgttgaaca
     1681 ggttcatgat ctggaacgag cgcgagatgc ccttgcgcgc aatggcacgc ggcgtgaggc
     1741 cggtgatgtc ttcaccggcg aaagtcacgc ggccgcgatc gggacggaac agcccggtca
     1801 gcacattgaa gcaggtggtc ttgcccgcgc cgttggggcc catgatgccg gccagctgcc
     1861 cttcctcgaa cgagaggctg atgtcttgca gcaccacctg gtcgccaaag cgcttggcca
     1921 ggccggcggc ttcgaacagt gccattcagg cctccctttc ctgcaccgcg accaccggcg
     1981 cagccggcct ttgccctggc cgtcgcgcgc gccaggcctg ccacatgccc gccacgccct
     2041 cgggcttgaa cagcaccatc accatgaagg ccaggccgta ccagagcaac caggtttcgg
     2101 tgtacgcgcc cagcaggtcg cgtgcgagga tgaagaacgc ggcgccgatc accgggcccc
     2161 agaaactgac caggccgccg ccgatcagca ccatcatcac cacgaagccc gagttgtgca
     2221 ggctcatgac attggggtag gccgactgct gcgccatcgc gaacaatccg cccgccaggc
     2281 cggccacacc ggccgagatc gtgaaggcca gccacttgta gagccagacg ttgtagccga
     2341 cgaatgccgc ccgggtttcg ttctgcttga tggcgctaag cacccggccg agcggggaat
     2401 ggatcagccg ccacagcgcc accaaggcca gcgcgaaaac ggcaaggcaa aaataaaaga
     2461 gcgcttcatt gctctgcagg tcgatggccg cgaagccgaa atccgcgggc agccgcttga
     2521 tgttgagcag cccgtcctcg ccgccggtga gccggtgcga tttgatcgcg atgaaccaga
     2581 acacctggcc gaaggcgatg gtcagcagcg catagtaaat gccgcggcgg tgcgaaatga
     2641 acaggcccac cagcgccccc aggatcacgg ccatggccac cgcccccagc agatcgaacc
     2701 agaggttggc ccacagcgtt ttctgcgcca gcccgaacgc gtaa
//
LOCUS       DASWIY010000398         2883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3238874, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000398 DASWIY010000000
VERSION     DASWIY010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3238874"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /gene="ppsA"
                     /locus_tag="VGA59_14015"
     CDS             <1..1572
                     /gene="ppsA"
                     /locus_tag="VGA59_14015"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX9720837.1"
                     /translation="VKTVEVPLEQRNRYSLTDAEVEELARYALVIERHYGRPMDIEWG
                     RDGLDGQLYILQARPETVKSQQHGKAEIRYKLKGKGNVLAEGRAIGQKIGTGPVRLVH
                     NSSEMDKVQAGDVLVTDMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVG
                     CGNATDRLKDGTLVTVSCAEGDTGFIYDGLLETEVTEVLRGEMPAIPVKITMNIGNPQ
                     LAFDFCQLPNHGVGLARLEFIINNNIGVHPKAILDYPNVDADLKKAVESVARGHASPR
                     AFYVDKVAEGVATIAAAFWPKPVIVRLSDFKSNEYRKLIGGSRYEPEEENPMLGFRGA
                     ARYISGEFREAFAMECEALRRVRTEMGLTNVQVMVPFVRTLGQADRVTQLLAQQGLKR
                     GEGGDGTAGSELKIIMMCEVPSNAILADEFLAFFDGFSIGSNDLTQLTLGLDRDSGLE
                     LLAADFDERDPAVKALLTRAIAACKAQGKYVGICGQGPSDHPDFARWLADQGISSISL
                     NPDSVIATWRQLAGE"
     gene            1704..>2883
                     /locus_tag="VGA59_14020"
     CDS             1704..>2883
                     /locus_tag="VGA59_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX9720838.1"
                     /translation="MADNPDYAAYKRRLARILTQYVVGLCVALGVLAWAERNGLSRQW
                     LGPIFLFATVMMYAAIGIYSRTADAEEYYVAGRRIPPFYNGMAAAADWMSAASFISLA
                     GGLYLQGFSGSDTQPGGLAYVMGWTGGFCLVALLVAPYLRRLGLYTVPDYFALRYGGR
                     WPRLIAAFAAMLCSFTYVVAQIYGIGLIASRLTGVQFEIGILLGLGGVLLCSFLGGMK
                     AITWTQVAQYVILLMAFLIPVSWLAYKQLGNPLAPLVYGKQLQKISEIEKTLIDSPAE
                     KQVQAEYKRRAAEYEARLADIEGSLRRERAAALQLIRRLKAQNADSAHIVAAHRELAA
                     LPREPSAARELWTRAMHESHDRSRPLGGLLPHSQPFAGDPDGTPAQREMFDTSRLNFL
                     A"
BASE COUNT          497 a          932 c          975 g          479 t
ORIGIN      
        1 gtcaagaccg tggaagtgcc gctggagcag cgcaaccgct attcgctcac cgacgcggag
       61 gtggaggaac tcgcgcgcta tgcgctggtc attgagcggc actatggccg tccgatggac
      121 atcgagtggg gcagggatgg cctcgacggc cagctgtata tcctgcaggc ccgtcccgag
      181 accgtgaaga gccagcagca cggcaaggcc gagatccgct acaagctcaa gggcaagggc
      241 aacgtgctgg ccgaaggccg tgccattggc cagaagatcg gcacgggacc ggtcaggctg
      301 gttcacaaca gcagcgagat ggacaaggtg caggccggcg acgtactggt gaccgacatg
      361 acggacccga actgggagcc ggtgatgaag cgggccagtg cgatcgtcac caaccgcggc
      421 gggcgcacct gccacgcggc catcatcgcg cgcgaactgg gcatccccgc cgtggtcggc
      481 tgcggcaacg ccaccgacag gctgaaggac ggcacgctgg tcacggtgag ctgcgccgaa
      541 ggtgacaccg gattcatcta tgacggcctg ctggaaaccg aggtcaccga ggtgctgcgc
      601 ggcgagatgc cggcgatccc ggtcaagatc acgatgaaca tcggcaaccc ccaactggca
      661 ttcgattttt gccagctacc caaccacggc gtggggctgg cccggctgga attcatcatc
      721 aacaacaaca tcggggtgca cccgaaggcc atcctcgact atcccaacgt ggatgcggac
      781 ctcaagaagg ccgtggaatc cgtggcgcgc ggccacgctt caccgcgcgc tttctacgtc
      841 gacaaggtgg ccgagggcgt ggccaccatc gcggccgcct tctggcccaa gccggtcatc
      901 gtgcggctgt ccgatttcaa gtccaacgaa taccgcaagt tgatcggcgg gtcgcgctac
      961 gagcccgagg aagaaaaccc gatgctgggt ttccgcggcg cggcacgcta catcagcggc
     1021 gagttcaggg aggcattcgc catggagtgc gaggcgctgc ggcgcgtgcg cacggagatg
     1081 ggcctgacga acgtccaggt gatggtgccc tttgtgcgca cgctcggcca ggccgaccgg
     1141 gtgacgcagc tgcttgcgca gcagggactc aagcgcggcg agggcggcga cgggaccgcc
     1201 ggctccgaac tgaagatcat catgatgtgc gaggtgccca gcaacgccat cctcgccgat
     1261 gaattcctcg cgttcttcga cggtttctcc atcggctcga acgatctcac gcagctgacg
     1321 ctgggactgg accgcgactc gggcctggaa ctgctggccg ccgatttcga cgagcgcgat
     1381 ccggcggtga aggcgttgct gacccgggcc atcgcggcct gcaaggcgca aggcaagtac
     1441 gtcggcatct gcggccaggg cccaagcgac caccccgatt tcgcgcgctg gctggccgac
     1501 caggggatct cctccatctc gctcaatccc gacagcgtga tcgccacctg gcgccaactg
     1561 gccggcgagt gacgcgttac gcaggcagct ctgggcaagc cgctcgacgc acttgttcag
     1621 agcttccctg gcggtaaccc tgttggccgg gtcggcccca gggtgcgatg atggcgcgct
     1681 ccgaggggct cgcgaaaatc acaatggccg acaaccctga ctatgccgcc tacaagcggc
     1741 ggctggcgcg catcctgacg cagtacgtcg tcggcctgtg cgtggcgctg ggcgttctgg
     1801 cgtgggccga acgcaacggc ttgtcgcggc agtggctggg gccgatcttc ctgttcgcca
     1861 ccgtgatgat gtatgcggcc atcggcatct atagccgcac cgcggacgcc gaggagtatt
     1921 acgtcgccgg ccggcgcatc ccgccgttct acaacggcat ggcggcggcg gccgactgga
     1981 tgagcgcggc gtccttcatc agcctggcgg gcgggctcta cctgcagggc ttctccggct
     2041 ccgacacgca accgggcggg ctggcctatg tgatgggctg gaccgggggc ttttgcctcg
     2101 tggcgctgct ggtcgcgcct tacctgcgcc ggctcgggct ttatacggtg ccggattatt
     2161 ttgcgctgcg ctatgggggc cgctggcccc gcctgatcgc cgcgttcgcc gccatgctgt
     2221 gctcgttcac ttatgtcgtc gcgcagatct atggcattgg cctgattgct tcgcgcctga
     2281 ccggcgtgca gttcgaaatc ggaatcctgc tggggctggg cggcgtcctt ctgtgctcgt
     2341 tcctgggtgg catgaaggcg atcacctgga cccaggtggc gcagtacgtg atcctgctga
     2401 tggccttcct gattccggtg tcctggctgg cctacaaaca gctgggcaac ccgctggcgc
     2461 cgctcgtgta cggcaagcag ttgcagaaga tcagcgaaat cgaaaagacg ctgatcgact
     2521 cgcccgccga aaagcaggtc caggccgaat acaagcggcg ggctgccgaa tacgaggccc
     2581 ggctggccga tatcgaagga tcgctgcggc gcgagcgcgc cgcggccctg caactcattc
     2641 gccggctcaa ggcacagaat gcggattcgg cccacatcgt cgcggcccat cgtgagctgg
     2701 cggcgctgcc gcgtgagccg tcggcggcgc gcgaactctg gacccgcgcc atgcacgaga
     2761 gccatgacag gtcgcggccc ctggggggcc tgctgcccca cagccagccg ttcgcgggcg
     2821 atccggacgg cacgcccgcg cagcgcgaaa tgttcgatac ctcgcggctt aatttcctgg
     2881 cac
//
LOCUS       DASWIY010000399         5383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_274119, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000399 DASWIY010000000
VERSION     DASWIY010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274119"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VGA59_14025"
     CDS             <1..395
                     /locus_tag="VGA59_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720839.1"
                     /translation="EIDMNRKLASTLAFASTTAAVLAFAAIASGNAYADDITIDNTPF
                     VSSKSRAEVQAEVMGQSGLLSNASSEWSLQMNGPAQPNSAYTREAAQAEYIASRDEVN
                     ARNAEDGGSSYFAPLPRSATVVMGGPAR"
     gene            819..2600
                     /locus_tag="VGA59_14030"
     CDS             819..2600
                     /locus_tag="VGA59_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9720840.1"
                     /translation="MSAPWTEVPFEFPRSTKVILVLDVVESVRLMEQDEHDFVQRWHF
                     FVEQARKILSLHGGRMHKSLGDGLMLEFDDAHGAIKSAFAMQELCRTGNQQRPPELHM
                     HLRVGAHLAEFVEDQYDIYGTDVNLTARIATLAGPGEIVVTAGLRDRLTAGLDADIED
                     LGECHLKHVHEPIRAYRVGPVGHAPVIERGSGIKMDLRPTIAVIPFAMRNAEPGHEML
                     GEALADEVIAALSRTTELHVISRLSTTVFRERHEAVADIRSHLGASYILSGVCRSMGN
                     QFTLFVELIDARTGNVGWADNLKGQVNGLFLTDNELINRLIGAISSAVMSVELQRARS
                     NALPTLEGYTLLLSAVALMHRTALGDFERARQMLDHLIQRSPRHPVPLAYLAHWHVLR
                     VSQGWSDSPGTDTKAAQDCTRRALDGDPHHPLALTVDGLVRTNLLQDPASATAQYEAA
                     IAENPNESLAWLLQGTMHAFIGDGEQAVHDTERALKLSPLDPLRYYYDSLAATAATSA
                     GHYARAIQLAERSLRQNRTHTSTYRAMAIAQALLGDTAGAQRTIQQLLKLQPDFSVSA
                     FLARSPSARYPIGQRYAAALRMAGLRE"
     gene            2631..3743
                     /locus_tag="VGA59_14035"
     CDS             2631..3743
                     /locus_tag="VGA59_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9720841.1"
                     /translation="MSGLSGLSGLSGLSGLSGLSGLSGLSGLAAYVDPALTQALIGGR
                     SALVGPFQGVTLPVDLPLPLDDPERLAQWDAIPRLQVCGLALLAGMRFSVNQNKESVT
                     LGFVDLDRKPLKDYSLVRLVRPSKAVFRQQLDLVANYAELREERASEILTQMVPQVPF
                     WASIVGLQAHRHKWTLELVALSLSLASHVEMRFKHAFACPRPVELSPQIQPMIPTPGH
                     ASWPCGHATEAYMVSGILQALLPNGATYGEQLERQAARISVNRTVAGLHYQVDNAVGR
                     LLGAALAEFFVARCIRNAKLHERGFIGPNFHGPKNTVIDFDPRVSMTDNKSGYYEYDK
                     SPISNGKSPSSTGSVACSPLLAFMWAEAAKEWQSLK"
     gene            3823..>5383
                     /locus_tag="VGA59_14040"
     CDS             3823..>5383
                     /locus_tag="VGA59_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720842.1"
                     /translation="MDPYLVWAEADQFAGYRDVLPKWLPIAMELHANSSIAQLTAAAT
                     TRWLTVPAVYTSGCAPAGLRFCTARVRPEFFAEIAKGGRLHAIVKRVELGLPAAPYND
                     QPAFQGDDKLPQTSNDIKPKVLSAAQQPLSGKVLGLIDDSLALAHANFLGQGGKPRTA
                     FFWRQDSEGKGRVPKDMAYGRELTAADIDAAMRCHSYNGLVDESAVYTALGLSVLGQK
                     WPHDHDPFHALDTAVSHGTHVMDLAGGPRTLLVQVSNLPPGYDAPPSWAPANDAASRS
                     PIVAVQLDYDAVADTSGGSLNVHVLDGLMYILSRCTAAAKIVVNISFGTLAGPHNGTS
                     LLEAAMDQLVSLCGNRLNIALAAGNSYQTRTHANVTLGKNQDATLRWCVLPDDSSQSF
                     LELWMPETPLGLEIEVTPPGREPLPALRLGESRMWLDGGKRPVCALIYPTRVATGEDA
                     TCALLALAPTFSLEPKAATAPSGVWQVRLNNTKTGNVTIDAYVERDDVVVGTRGGAKQ
                     SHFEDEPQLPYS"
BASE COUNT         1042 a         1708 c         1664 g          969 t
ORIGIN      
        1 aggagatcga catgaaccgc aaactcgcat ccaccctcgc cttcgccagc acgaccgctg
       61 ccgtcctcgc cttcgctgca atcgcttcag gcaacgccta cgcggacgac atcaccatcg
      121 acaacacgcc cttcgtcagc agcaagtccc gcgccgaggt gcaggccgaa gtcatggggc
      181 aatccggact cctgagcaat gcgtccagcg agtggtcgct gcagatgaac gggccagccc
      241 agcccaacag cgcttatacg cgtgaggcag cgcaggccga atacatcgct tcgcgggacg
      301 aagtcaatgc gcgcaacgca gaagacggcg gctcgtccta tttcgcacct ctgccgcgca
      361 gcgccaccgt tgtcatgggc ggtccggcgc gttgaaggcg atccagcgcc cgaccgcgtg
      421 ctgccaatcg acagccgtgc gcttttccgg acgcactgac aagcccaccc gcccgccgcg
      481 ccggcgatac cagactcgca cccacagcca gtcaggttga accaggcgcg gcgcgctgct
      541 tcgggcgcgg taaacaaggg tcgcggctca cacattgagc gataacgcga agcattggtg
      601 aagaaaggag gcccacgatg caattgaatc gtgcgcttca gggccaacaa ggatttcgcg
      661 taaggctcct gctcgttggc gagcggggaa atatgagttg ctgcctctgc catggtagag
      721 cttcgacaac ccagctgtca ctcggacgag aagatgtctt tccagatgac gttttctcct
      781 acagaattgg gaggccgttt ctcgctgtaa cgcctacgat gtctgctccc tggactgaag
      841 tacctttcga gtttccgcgc tctaccaagg tgatcctggt cctggacgtg gttgagtcgg
      901 tccgtctgat ggagcaagac gaacatgatt tcgtgcagcg ctggcacttc tttgtcgagc
      961 aggcaaggaa aattctgtca ttgcacggcg ggcgaatgca caaaagcctg ggcgacggtc
     1021 tgatgctgga gttcgacgat gcgcacggcg ccatcaagag cgcttttgcg atgcaggagc
     1081 tttgccgcac cggcaaccag caacggccgc cagagcttca catgcatttg cgggtcgggg
     1141 ctcatctggc ggaattcgtg gaggaccagt acgacatcta cggcacggac gtgaacctga
     1201 ccgcgcgtat tgccacttta gcggggccgg gcgagatcgt ggtgactgca ggcctgcgcg
     1261 acaggttgac ggcgggactc gatgccgaca ttgaggatct cggtgaatgc cacctgaagc
     1321 atgtgcatga gccgatacgg gcctaccgtg tcggcccggt cgggcatgcg ccggtgatcg
     1381 agcgtggaag tggaataaaa atggatttgc gcccgaccat cgcggtgatt ccgtttgcca
     1441 tgcgcaacgc ggagcccggc cacgaaatgc tgggcgaggc actggccgat gaggtcatcg
     1501 cggcgctttc ccgcaccacc gaactgcatg tgatctcgcg cctgtcgacg accgttttcc
     1561 gggagcggca tgaggccgtc gcggacatcc gcagccattt gggcgcgagt tacatcctgt
     1621 ctggcgtctg ccgcagcatg ggcaatcagt tcacgttgtt cgtggaactg atcgacgcca
     1681 ggactggtaa tgtgggttgg gcggacaacc tcaagggcca ggtcaatggc ttgttcctga
     1741 ccgacaacga gctgatcaac cgcctcatcg gcgccataag ctcggccgtt atgtccgtcg
     1801 aactgcagcg ggcacgcagc aatgcgctgc ccacgctcga aggctacacg ctgcttctca
     1861 gcgccgtggc gctcatgcat cgcacggcgc tcggcgattt tgaaagagca cgccagatgc
     1921 tcgaccactt gatccagcgc agcccacgtc atcctgttcc gctggcctat ctggctcact
     1981 ggcacgtgct gagggtatcg caaggatggt ccgacagccc gggcactgac accaaggcgg
     2041 cccaggattg caccagacgc gcactggatg gcgacccgca ccatccactg gcgctcacgg
     2101 tcgacgggtt ggttcgcacg aatctcctgc aagatcccgc ctcggcgact gcccagtacg
     2161 aggcggccat cgcggagaac cccaacgagt cactggcctg gctgcttcag gggacgatgc
     2221 atgccttcat cggggacggc gagcaagctg tacacgatac ggagagagca ttgaagctct
     2281 ctccgcttga ccccctgcgg tactactacg actcactcgc ggcaaccgcg gcgacatcgg
     2341 ccggccacta tgcaagagct attcagctgg ccgagaggtc gctcaggcag aaccgcacgc
     2401 acacctcgac ctatcgggcc atggcgatag cgcaggctct cctaggggac accgcggggg
     2461 cgcagcgcac catacaacag cttcttaagc tccaacccga cttttcagtc tcagccttcc
     2521 tcgcgcggtc gccaagcgcc agatatccga taggccagcg ctacgccgcg gccttgagga
     2581 tggctggcct gcgtgaatga caacaaacac atgcacgaat ggagtagcac atgagcggtt
     2641 tgagcgggtt gagtggcctc agtggtttga gtggcctcag cgggttgagt ggccttagcg
     2701 gattgagcgg cctcgcagcg tatgtcgacc ccgctctcac ccaggccttg atcgggggcc
     2761 gtagcgcatt agtcggcccc ttccagggcg tcacgttgcc cgtggatctt ccccttcccc
     2821 tggacgatcc cgagcggctt gcgcaatggg acgcgatacc tcgtctccag gtatgcgggc
     2881 tggcgctgtt ggcgggcatg cgcttttccg tcaaccagaa caaggagtcc gtcacacttg
     2941 gttttgtcga tctcgaccgg aagcctctga aggattattc gctcgtgcgg ctcgttcgcc
     3001 ccagcaaggc cgtgttcagg caacagctgg atctggtagc gaactatgcc gagctgagag
     3061 aagagcgcgc cagcgaaatc ctcacgcaga tggtgccgca ggtgcctttc tgggcatcga
     3121 tcgttggtct gcaggcgcat cgtcacaagt ggaccctgga actggtggcg ctgtcactct
     3181 cgctggcctc acatgtggag atgcgcttca agcatgcctt cgcctgtccg aggccggtgg
     3241 aactgtcccc gcagatccag ccgatgatcc cgacgcccgg gcatgcgagc tggccctgcg
     3301 ggcacgccac cgaagcgtac atggtgtccg gcatactgca ggcgctgttg cccaacggag
     3361 ccacctacgg cgagcaactc gagcggcagg cggcgcggat ttcagtcaac cgcactgttg
     3421 cgggcttgca ctaccaggtg gacaacgccg tggggcgcct gctcggggct gcgctggcgg
     3481 aattcttcgt cgcccgctgc attcgcaatg ccaagctgca cgaacggggt ttcatcgggc
     3541 ccaacttcca tggcccgaag aacacggtca tcgacttcga ccctcgcgtt tccatgacgg
     3601 acaacaagag cgggtattac gagtatgaca aatctcccat ttcgaatggc aaatctccct
     3661 cttcgactgg cagcgttgcg tgctccccgt tgctcgcctt catgtgggcc gaagccgcaa
     3721 aagagtggca gtcgctgaag taggccggcc atgacctgga tgcctctgcc tacgggcagt
     3781 tttacgggaa tcgactgggc caggccgcac gcgacagccg gcatggatcc ctacctcgtc
     3841 tgggccgaag ccgaccagtt cgccggctac agggacgtcc tccccaaatg gttaccgatc
     3901 gccatggaac tgcatgcgaa ctccagcatc gcgcaattga ccgcggcggc gaccaccagg
     3961 tggctaaccg tccctgccgt ttacacctcg ggctgcgccc ccgcgggact gcgattttgc
     4021 acggcacgag tccggcccga gtttttcgct gaaatcgcca agggggggcg ccttcacgca
     4081 atcgtgaagc gggtagagct gggcttgccc gcggcgccgt acaacgacca gcccgcattc
     4141 cagggcgacg acaagctgcc gcagacttcg aacgacatca agcccaaggt cttgtcagcc
     4201 gcgcagcagc cgctgagcgg caaggttctg ggcctcatcg acgacagtct tgccttggct
     4261 catgccaact tcctggggca aggcggaaag ccccgcacag cttttttctg gcggcaagac
     4321 agcgaaggaa agggccgcgt gcccaaggac atggcgtatg ggcgcgagct gacggctgcg
     4381 gacatcgatg cagccatgcg ttgccacagc tacaacggcc tcgtggacga aagcgcggta
     4441 tacacggcat tgggcctttc cgtactcggc cagaaatggc cgcatgacca tgacccgttc
     4501 catgcgctcg acacggccgt gagccacggc acgcacgtga tggacctggc cggtggcccg
     4561 cgaaccctgc tggtgcaagt ttccaacttg ccaccgggtt acgatgcgcc gccgagctgg
     4621 gcgccggcga atgacgctgc cagccggtcc cccatcgtcg cggtgcaact ggactacgac
     4681 gctgtggccg acacgtcggg cgggtcgctg aacgtgcacg tgctggacgg gctgatgtac
     4741 atcctgtctc gctgcacggc cgccgccaag atcgtcgtga acatcagctt cggcaccttg
     4801 gcgggcccac acaacggcac atcgctgctg gaagccgcga tggaccagct cgtctccctg
     4861 tgcggaaacc gactgaacat cgcgctggct gcgggcaaca gctatcagac ccgcacccat
     4921 gccaatgtca cgctcggcaa aaaccaggac gcgacgcttc gctggtgcgt gctgcccgac
     4981 gattcgagcc agagcttcct tgagctctgg atgcccgaaa cccccttggg tctggagatc
     5041 gaggtcacgc cgcccggtcg cgagccgctg ccggcattgc gcctggggga gtcgcgcatg
     5101 tggctggatg gcggcaaacg ccccgtatgc gcattgatct atccgacgcg ggtagccaca
     5161 ggcgaagacg caacctgcgc cttgctggcg ctggcaccca cgttcagcct ggagcccaag
     5221 gcggcaacgg caccaagcgg ggtctggcag gtgcgcctga acaacacgaa aaccggcaat
     5281 gtgacgatag atgcctatgt ggagcgtgac gacgtagtgg tcggaacgcg cggcggagcg
     5341 aaacagtccc acttcgagga tgagccgcaa ttgccatact ctc
//
LOCUS       DASWIY010000400         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2145628, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000400 DASWIY010000000
VERSION     DASWIY010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2145628"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VGA59_14045"
     CDS             <1..248
                     /locus_tag="VGA59_14045"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9720843.1"
                     /translation="AQTGLLGLVAGFVAAPLGVALAGLLVFVINERAFGWSMTFAVPP
                     GVLAGGILMAVGAALAAGVLPALRLSRMPLAAALREE"
     gene            259..1359
                     /locus_tag="VGA59_14050"
     CDS             259..1359
                     /locus_tag="VGA59_14050"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX9720844.1"
                     /translation="MRRLLGLVLASAAAAAMAQVDVSGLLGSRPAPGFARATAPRAFS
                     FPADHGPHPDFRSEWWYFTGNLAAADGRHFGFQLTFFRVALAPDAAPRASAWATDQAW
                     MAHFTLTDTESGRFHAAERLGRGALSITGAQASPFRVWVDDWQAMSRASTGLFPLRLS
                     ASHGDVAVELELSATKPLVLQGRQGWDRKGPESGNASYYYAYTRLAAQGRVRTRDARF
                     DVTGTAWMDREWSTSALGPDLAGWDWISLQLSDGRELMLYRLRRKDGGASAFSKGALI
                     LPTGARIALSLREFELEPHRWWTSPTTGVRYPVAWSIEIPSLELSLDIAPRLDDQELD
                     LSVRYWEGAVLAKGRVNAAPVTGQGYLELTGY"
     gene            complement(1426..1541)
                     /gene="rrf"
                     /locus_tag="VGA59_14055"
     rRNA            complement(1426..1541)
                     /gene="rrf"
                     /locus_tag="VGA59_14055"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1647..>1834)
                     /locus_tag="VGA59_14060"
     rRNA            complement(1647..>1834)
                     /locus_tag="VGA59_14060"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          271 a          605 c          620 g          338 t
ORIGIN      
        1 tcgcccagac ggggctgctc ggcctggtcg cgggattcgt tgcggctccg cttggcgtcg
       61 cgctggccgg cctgctggtc ttcgtcatca acgagcgcgc cttcggctgg agcatgacct
      121 tcgcggtccc gcccggcgtg cttgcaggcg gcatcctgat ggcggttggc gccgcactcg
      181 ctgcgggcgt tctgcccgcg ctgcggttat cgcggatgcc gctggccgcg gcgctgcgcg
      241 aggaatagag aggaaccgat gcgccgcctc ctcggtctgg tgcttgcctc ggccgcagcg
      301 gcggccatgg cgcaggtgga cgtgagcggt ctcctcggca gccggccggc accgggcttt
      361 gcgcgggcca cggcgccgcg cgcattctcc tttccggcgg atcacggccc gcatcccgat
      421 ttccgcagcg agtggtggta cttcacgggc aacctcgcgg ccgcggacgg gcgccatttt
      481 ggcttccagc tcacgttctt ccgcgtcgcg ctggcgccgg atgccgcccc gcgcgcatcc
      541 gcctgggcga ccgaccaggc gtggatggcg catttcacgc tgaccgacac cgaatccggc
      601 cggttccatg cggccgagcg gctcgggcgg ggcgcgctca gcatcaccgg cgcacaggcc
      661 tcgccgtttc gggtgtgggt cgatgactgg caggcgatga gccgcgcgtc gacgggcctg
      721 ttcccactgc ggctcagcgc gtcgcatggc gacgtggccg tcgagctcga gctttcggcg
      781 acgaagccgt tggtcctgca aggtcggcag ggatgggatc gcaaggggcc ggagtccggc
      841 aacgcctcgt actactacgc ctatacgcga ctcgcggcac agggacgcgt gcgcacgcgc
      901 gatgcgcgct tcgacgtgac cggaacggcc tggatggatc gcgaatggag cacgagcgcg
      961 ctcggcccgg acctcgccgg ctgggactgg atctcgctgc aactgtcgga cggccgcgaa
     1021 ctcatgctct atcggctgcg caggaaggac ggcggcgcca gcgccttcag caaaggcgca
     1081 ttgatcctgc cgaccggcgc acggatcgcg ttgtcgcttc gcgaattcga gctcgagccg
     1141 caccgatggt ggaccagtcc gaccaccggt gtgcgctatc ccgtcgcgtg gtccatcgag
     1201 attccgtcgc tcgaattgtc gctcgatatt gcgccccgac tcgatgacca ggagctcgac
     1261 ttgtcggttc gctactggga gggcgccgtg ctcgcgaaag ggcgcgtgaa tgccgcgccg
     1321 gtcacgggcc aggggtatct ggagctgacg ggctactgag ccttgcggcc gcctgcgccg
     1381 aaaagaaaaa ccccccgacg tttccgtcgg ggggttctgt tgggatcctg gcagtgacct
     1441 actctcacct ggcctacggc caaactacca tcggcgcaga gcgttttcac ttccgagttc
     1501 ggaatgggat cgggtggttc ccgcccgcta tgaccgccag gaaaactgtc acccgcgccg
     1561 ctgatcgcag ccacgcaggc atgtacggaa actgtagata aagcagatgt gtcgcatcca
     1621 cggagctcag gctccttggg tgttatatgg tcaagcctca cgggcaatta gtactggtta
     1681 gctgaacgca ttgctgcgct tacacaccca gcctatcaac gtcgtagtct tcgacggccc
     1741 ttcaggagga tcaagtcctc agggagatct catcttgggg ggggcttccc gcttagatgc
     1801 tttcagcggt tatcccgtcc gcgcatagct accc
//
LOCUS       DASWIY010000401         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1381844, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000401 DASWIY010000000
VERSION     DASWIY010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381844"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /gene="adh"
                     /locus_tag="VGA59_14065"
     CDS             <1..1273
                     /gene="adh"
                     /locus_tag="VGA59_14065"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX9720845.1"
                     /translation="LLRIADRIDQNLELLAYAETVDNGKAIRETLNADIPLSSDHFRY
                     FAGCLRAQEGSLSEIDGDTIAYHFHEPLGVVGQIIPWNFPILMAAWKLAPALAAGNCI
                     VLKPAESTPVSIMVLVELIADLLPPGVLNIVNGLGREAGMPLATSKRIAKIAFTGSTA
                     TGRVIAQAAAANLIPATLELGGKSPNIFFDDVAAADDDFFDKAIEGLVLFAFNQGEVC
                     TCPSRALIQESVYDKFMERALSRVRAIKQGSPLDTDSMMGAQASIEQMKKIESYLALG
                     KEEGAQVLIGGSRAKLPGALAEGYYIQPTLFKGHNKMRIFQEEIFGPVLAVTTFKDEA
                     DALAIANDTPYGLGAGVWTRDGTRAYRMGRAIQAGRVWTNCYHAYPAHATFGGYKESG
                     IGRETHKVMLDHYQQTKNLLVSYSPKALGFF"
     gene            complement(1343..1984)
                     /gene="nth"
                     /locus_tag="VGA59_14070"
     CDS             complement(1343..1984)
                     /gene="nth"
                     /locus_tag="VGA59_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX9720846.1"
                     /translation="MQQKHIEPFFATLRAANPLPASELEFTSPFELLVAVLLSAQATD
                     VSVNKATRKLFPVASTPQAILDLGPERLEGYIKTIGLYRGKGRNLLATCRILVDQYGG
                     EVPRTREALEALPGVGRKTANVILNVAFGEETMAVDTHIFRVCNRTGLAPGKNPLQVE
                     QALMKRVPKEYRVDAHHWLILHGRYVCVARKPKCWQCAVIQYCDYQPKTPPPK"
     gene            complement(2068..>2257)
                     /locus_tag="VGA59_14075"
     CDS             complement(2068..>2257)
                     /locus_tag="VGA59_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9720847.1"
                     /translation="WAGLRPATPGNVPIIGRGRPANLFYNTGHGTLGWTLACGSGQAI
                     ADIVGGRRPARQLPFRGM"
BASE COUNT          398 a          757 c          711 g          391 t
ORIGIN      
        1 cctgctcagg atcgccgacc gcatcgacca gaacctcgag ttgctggcct atgccgagac
       61 ggtggacaac ggcaaggcga tccgcgagac gctcaacgca gacatcccgc tgagctcgga
      121 ccatttccgc tatttcgcgg gttgcctgcg cgctcaggaa ggcagcctga gcgagatcga
      181 cggcgacacc atcgcttacc atttccatga accgctgggc gtcgtgggcc agatcatccc
      241 ctggaacttc ccgatcctga tggcggcctg gaaactggcg ccggccctgg ccgcgggcaa
      301 ctgcatcgtg ctcaagcccg ccgaatcgac gccggtgtcc atcatggtgc tggtcgagct
      361 gatcgccgac ctgctgccgc cgggcgtgct caacatcgtc aatggcctgg ggcgtgaagc
      421 gggcatgccg ctggccacca gcaagcgcat cgccaagatc gccttcaccg gttctaccgc
      481 cactggccgt gtcatcgcgc aggcagcggc cgccaacctg atcccggcca cgctggaact
      541 cggcggcaaa tcgcccaaca tcttcttcga cgacgtcgcc gcggccgacg atgatttctt
      601 cgacaaggcc atcgaaggcc tggtgttgtt cgccttcaac cagggcgagg tctgcacttg
      661 cccgtcgcgc gcactgatcc aggaatcggt ctacgacaaa ttcatggagc gcgcgctgtc
      721 gcgggtgcgg gccatcaagc agggcagccc gctcgacacc gacagcatga tgggcgccca
      781 ggcttccatc gagcagatga agaagatcga gtcctatctc gcactcggca aggaagaggg
      841 cgcgcaggtg ctgatcggcg gcagccgcgc caagctgccc ggcgcgctcg ccgagggcta
      901 ctacatccag ccgaccctgt tcaagggcca taacaagatg cggatcttcc aggaggaaat
      961 cttcggcccg gtgctggccg tgaccacctt caaggacgaa gccgacgccc tggcgatcgc
     1021 caacgacacg ccgtacggtc tgggcgccgg tgtgtggacg cgcgacggca cgagggccta
     1081 ccgcatgggc cgcgccatcc aggcgggccg tgtgtggacc aactgctatc acgcctatcc
     1141 ggcgcacgcc accttcggcg gctacaagga gtcgggcatc ggccgcgaga cccacaaggt
     1201 catgctcgac cattaccagc agaccaagaa cctgcttgtg agctacagcc cgaaggcgct
     1261 cgggttcttc taagccagcc tcgcgttgcg gccctttaat tggggtcaga ttccaattaa
     1321 attaattgga atctgacccc aattatttgg gcggcggcgt cttcggctgg tagtcgcaat
     1381 actggatcac cgcgcactgc cagcacttcg gcttgcgcgc gacgcacaca tagcgcccgt
     1441 gcaggatcag ccagtgatgc gcatcgaccc ggtattcctt gggcacgcgc ttcatcaagg
     1501 cttgctccac ctgcagcgga ttcttgcccg gcgccagtcc ggtgcggttg cagacacgga
     1561 agatgtgcgt gtccacggcc atcgtctctt cgccgaacgc cacgttcagg atcacgttcg
     1621 cggtcttgcg ccccacaccg ggcagcgcct ccagcgcttc gcgcgtgcgc ggaacctcgc
     1681 cgccgtattg gtccaccagg atgcggcagg tggccagcag gtttcgcccc ttgccgcggt
     1741 acaggccgat cgtcttgata tagccctcga gccgctccgg gcccaggtcc aggatcgcct
     1801 gcggcgtgct cgcgaccggg aacagcttgc gggtcgcctt gttgacgctc acgtcggtgg
     1861 cctgcgcgga gagcagcaca gccaccagca attcgaacgg tgaggtgaat tccagctcgc
     1921 tcgccggcaa cgggttggcc gcgcgcaggg tcgcgaagaa cggttcgatg tgcttttgtt
     1981 gcatgtcagg gctgcctggc cgcggaaagt ccgctgccgc atcccaaagg ttaccttgtc
     2041 gtgcgccgcc tgcgagagtc gacagtctta catcccgcga aacggtaatt gccgcgccgg
     2101 ccggcgcccg ccgacgatgt cggcgatggc ctggccggag ccgcaagcca gcgtccagcc
     2161 aagggtgccg tggccggtgt tgtagaacag attggccggc ctgccgcggc cgatgatcgg
     2221 gacgttgccc ggcgtggccg gccgcaatcc ggcccag
//
LOCUS       DASWIY010000402         6370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2876026, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000402 DASWIY010000000
VERSION     DASWIY010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2876026"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..1273)
                     /locus_tag="VGA59_14080"
     CDS             complement(329..1273)
                     /locus_tag="VGA59_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEX9720848.1"
                     /translation="MNLHQFRFVQEAVRRNLNLTEAAKALHTSQPGVSKAIIELEEEL
                     GVEIFARHGKRLKRVTEPGQHVLKSIELIMREVGNLKRIGEQFSAQDSGTLSIATTHT
                     QARYVLPVPVAKLREAYPKVNVSLHQGSPDQVARMVIDEVAEIGIATESLSAYQELVT
                     LPCYEWEHVLVLPLGHPLTKKERINLEDLAAEPLITYHPSFTGRTRIDAAFAHKKLQP
                     RIALEAIDSDVIKTYVRLGLGIGIVAEMAVRDDGSNNDLAVRPLGNLFGQNLARVAFK
                     RSAYLRNFVYKFAELLSDRLDRNLIAKAMSGHPNDYEL"
     gene            complement(1348..1710)
                     /locus_tag="VGA59_14085"
     CDS             complement(1348..1710)
                     /locus_tag="VGA59_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEX9720849.1"
                     /translation="MSRHAIILLGHGSRDPLWRRPIEAVAERLKRRHADLAVCCAYLE
                     LEPPDLATAAARLAAGGAQRVTIVPMFLGTGKHGRDDPPRLLELLRQTHPAVEFDLRP
                     PVGDDPRVLDLLAVIAAE"
     gene            complement(1715..2815)
                     /gene="lptG"
                     /locus_tag="VGA59_14090"
     CDS             complement(1715..2815)
                     /gene="lptG"
                     /locus_tag="VGA59_14090"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX9720850.1"
                     /translation="MRTIRRLIYREVLAAVVFVAVGFLSLFFFFDFVDELPNVGKAGA
                     AYRLTQALAYVTLMVPNHLYELLPIAVLIGTIFVMARLAQSSEYTILRTSGLGPWRAL
                     RTLLALGLIFALLTFAVGDYLAPVADRAAQLLKARLGGRLSIGQTGAWLKEKQTFHSY
                     NVNVGALSPDGDMQGVRIFESDSRGFLVSITQAPKGHFALDGAWLLQDAERTEFGPGG
                     DVARVDRTSSPILRWPTEITPEMVSVALLKPDRMSTIDLFAYIRHLEANGQTSQRYEI
                     EFWRKVFYPLSCIVMVVLALPFAYLHFRSGGITSYVFGGVLIGISFFLLNNVFGYIGN
                     LQNWQPWLTAAAPGLIYSLFSLGAFGWLVLRR"
     gene            complement(2812..3921)
                     /gene="lptF"
                     /locus_tag="VGA59_14095"
     CDS             complement(2812..3921)
                     /gene="lptF"
                     /locus_tag="VGA59_14095"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX9720851.1"
                     /translation="MLFHSSLRKELARSFGATLVVLVTIVMTMTLIRTLAQATRGSVN
                     PQEVLMVMGYTVLGYLPPILTLSLFVAIVGTLSRMYRDSEMVIWFAAGRGLSAFLGPL
                     FRFAWPVLAVVAALALIVWPWANQQTQELKERYGKRGDLERVAPGQFQESASGTRVFF
                     LDKDAPDNKTGKNIFISTREHGKETVTSARSGRIEGVGDGQFLVLADGQRLESNGEGQ
                     LRISEFQELANRIGAIELGVRDVGPAKARSTAALVMEPTAVHLGELAWRLGLALAAMN
                     FVVIAITVSSANPRAGRSGNLVFALFAFVVYFNLLNLGQSWVSSGQVSFGVLLLTLHG
                     GVLLAGLLWLAKQHNNWTLRQLLRRHSRAKQEVAP"
     gene            3961..5445
                     /locus_tag="VGA59_14100"
     CDS             3961..5445
                     /locus_tag="VGA59_14100"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX9720852.1"
                     /translation="MDFELKTLGLAGAAGAKCDALVLLVGETFKPGKDALSKLAADAL
                     KARDLEAKPGKLLQAYRTNGIAAPRVLLAGCGDGSGKRVHAAVQAAVTSLRTSGAKRL
                     VICLPQDADEDAVRAAVLATAETSYVYVTTKSKPEGRELQRVTVAVADAARHRTGFAA
                     AEAAANGIEFARELGNLPPNLATPTRLGEEARKLAKAHGLQCEVLGPKEVAKIGMGSF
                     MAVAKGSDQPLRFIVLKYHGAAQSEAPVVLVGKGITFDSGGISIKPSQDMDEMKFDMG
                     GAASVLGTFHAIGELRPAINVVGLIPSCENMPDGRSVKPGDIVASLSGQTIEILNTDA
                     EGRLILCDALTYAERFKPRAVIDIATLTGACVVALGGVRSGLFATDETLAQALLDAGD
                     AALDPCWRMPLDDEYADGLKSNFADVANVAGRSGGAVNAAKFLQRFAGNYPWAHLDIA
                     GTAWKSGAAKGSTGRPVGLLVQFLLGQQSATPKPARKGGKRKAA"
     gene            5736..6173
                     /locus_tag="VGA59_14105"
     CDS             5736..6173
                     /locus_tag="VGA59_14105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX9720853.1"
                     /translation="MTEVTFHFNAPDKVGHACRLLRKAVASGAKVVVTGDAVLLRELD
                     VALWTFAPLEFVPHCLSGASAPAVLAASPVVLADSARSAPHQHVLLNLGAGVPEGFER
                     FERLIEVVTGQEDDRQQGRRRWKHYADRGYAIKGEDLGAKETR"
     gene            6173..>6370
                     /locus_tag="VGA59_14110"
     CDS             6173..>6370
                     /locus_tag="VGA59_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720854.1"
                     /translation="MANRKPPRYVPTLTEVVGTAGESKLAESAGISQEQIIQRVMQRI
                     DLTLERRLREAIAATVLEQTRS"
BASE COUNT         1152 a         2031 c         2113 g         1074 t
ORIGIN      
        1 caaaattgct acgcaattcc tgatctgtcc cgcaaggtca ctgtcttgtt gtcattgcgg
       61 gcttgacccg caatccatgg atcccggatc gagtcccgcc ttgtcattgc gggctttgac
      121 ccgcaatcca tggatcccgg atcgagtccg ggatgacagt tgatgaagac agagcaaaat
      181 tgctacgcaa ttcttgatct gtcccgcaag cttaatcggt tgaggacaga gcaaaattgc
      241 tccgcaattc ttgatctgtc ccggaaatct taaacagttg aggacagagc aaaattgctc
      301 cgcaattctt gatctgtccc acaaagtctc acaattcata atcattcggg tggccgctca
      361 tggctttggc gatcaggttc cggtctagcc ggtcggacag cagctcggcg aatttgtaga
      421 cgaaattgcg caggtaggcg ctgcgcttga aggcgacacg cgccaggttc tgcccgaaca
      481 ggttaccgag cggccgcaca gcgaggtcgt tgttgctgcc gtcgtcgcga accgccattt
      541 cggcgacgat gccgatgccc aggcccagtc tcacataggt cttgatcacg tccgagtcga
      601 tcgcctcgag ggcgatgcgc ggctgcagct tcttgtgggc gaaagccgcg tcgatgcggg
      661 tgcgtcccgt gaaggaaggg tgataggtga tgagcggttc ggccgccagg tcttccaggt
      721 tgatgcgttc cttcttggtc agcggatgac cgagcgggag caccagcacg tgttcccact
      781 catagcaggg cagggtcacc agctcctggt aggcggacag ggactcggtc gcgataccga
      841 tctcggcgac ctcgtcgatc accatgcgcg ccacctggtc aggcgagccc tggtgcaggc
      901 tgacgttgac ctttggatac gcctcgcgca gtttcgccac cggcaccggc agcacatagc
      961 gcgcctgggt gtgggttgtt gcgatcgaga gcgtgccgct gtcctgggcg ctgaactgct
     1021 cgccgatgcg tttgaggttg ccgacctcgc gcatgatcag ctcgatgctc ttgagcacgt
     1081 gctgcccggg ctcggtgacg cgcttgagcc gctttccgtg gcgggcgaag atctcgacac
     1141 cgagttcttc ttcgagttcg atgattgcct tggacactcc gggctgcgag gtgtgcaggg
     1201 ccttggccgc ctcggtcagg ttcaagttcc ggcgcacggc ttcctggacg aatcggaatt
     1261 gatggagatt cataccgaat ttcttctaag agagaaattc attatgccgc acaacgtcta
     1321 tgctgcgtct ccatcccatc gtgcctgtta ctctgccgcg atgaccgcga ggaggtcgag
     1381 gactcgcggg tcgtcgccga caggcggccg gagatcgaac tccacggccg ggtgggtctg
     1441 gcgcagcaac tccagcaggc ggggcgggtc gtcgcgcccg tgcttgcccg tgccgaggaa
     1501 catcggcacg atcgtgaccc gctgcgcccc gccggcagcc agtcgcgcgg cggctgtcgc
     1561 cagatccggc ggttccagct ccagatacgc acagcacacc gcgaggtccg catgccggcg
     1621 cttcagccgc tcggccacag cctcgatcgg ccggcgccag agcgggtcgc gcgaaccatg
     1681 cccgagcagg atgatggcgt gccggctcat tgctctacct tcgcagcacg agccagccga
     1741 acgcacccag cgaaaagagg gaatagatga gcccgggcgc ggccgcggtc agccagggct
     1801 gccagttctg caggtttccg atgtagccga acacgttgtt cagcaggaaa aagctgatgc
     1861 cgatcagcac tccgccgaaa acgtagctgg tgatgccgcc ggagcggaaa tgcaggtacg
     1921 cgaagggcag cgccagcacc accatgacaa tgcagctcaa gggatagaac accttgcgcc
     1981 agaactcgat ttcatagcgc tgcgaggtct ggccgttggc ttcaaggtgg cggatatagg
     2041 cgaacaggtc gatcgtgctc atccggtccg gcttgagcag cgccaccgac accatctccg
     2101 gcgtgatctc ggtgggccac cgcaggatgg gcgagctagt gcggtctacc cgcgcgacgt
     2161 cgccgccggg cccgaattcg gtgcgttcgg catcctgcag cagccaggcg ccgtcgaggg
     2221 cgaaatggcc cttgggggcc tgcgtgatcg acaccaggaa accgcgcgag tcggactcga
     2281 agatccgcac gccctgcatg tcgccatccg gcgagagggc gcccacgttg acgttgtagg
     2341 aatggaacgt ctgcttttcc ttgagccagg cgccggtctg gccgatgctc aggcgccctc
     2401 cgagccgggc cttgagcaac tgggccgccc ggtccgccac gggtgcgagg taatcgccga
     2461 cggcaaaagt gagcagcgcg aagatcaggc ccagggccag caaggtgcgc agcgcccgcc
     2521 acggccccag cccgctggtg cgcaggatgg tgtattcgga gctttgcgcg agccgcgcca
     2581 tgacgaagat ggtgccgatc agcacggcga tcggcagtaa ctcgtacagg tggttgggca
     2641 ccatcagggt gacgtaggcc agcgcctggg tcagccggta agccgcgccg gccttgccta
     2701 cattgggcaa ctcgtcgacg aaatcgaaga agaaaaagag cgacaggaag cccacggcca
     2761 cgaacaccac cgcggccagc acctcgcggt agatcagccg ccggatcgtc ctcatggcgc
     2821 aacctcctgc ttggcgcggc tgtggcggcg caggagctgc cgcaaggtcc agttgttgtg
     2881 ctgcttggcc agccagagca gtccggcaag cagcacgccg ccgtgcagcg tcagcagcaa
     2941 tacgccgaag ctcacctgcc cggagctgac ccagctttgc cccaggttca gcaggttgaa
     3001 gtacacgaca aaagcgaaca gtgcgaatac aaggttgccg ctgcggcccg cccgtgggtt
     3061 ggcgctcgac acggtgatgg cgatcaccac gaaattcatc gcggccagcg ccagtcccag
     3121 ccgccaggcg agctcgccca ggtggaccgc ggttggctcc atcaccagtg cggccgtgct
     3181 gcgggccttc gccgggccaa cgtcgcgtac cccaagttcg atggcgccga tgcggttggc
     3241 cagctcctgg aattcgctga tcctgagctg cccctctccg ttgctctcca gacgctgccc
     3301 gtcggcgagc accaggaact ggccgtcgcc gacgccctcg atgcggccgc tgcgcgccga
     3361 cgtgacggtt tcctttccgt gttccctggt cgaaatgaag atgttcttgc cggtcttgtt
     3421 gtccggcgcg tccttgtcca ggaaaaacac gcgcgttccg ctggccgatt cctggaactg
     3481 gccgggcgcc acgcgttcga ggtcgccgcg cttgccatag cgctccttga gctcctgcgt
     3541 ctgctggttc gcccagggcc agacgataag cgccagcgcc gccaccaccg ccagcaccgg
     3601 ccaggcaaag cggaacaggg gtcccaggaa ggccgacaag ccgcgccccg ccgcgaacca
     3661 gatcaccatt tcgctgtcgc ggtacatgcg cgagagcgta ccgacgatgg cgacgaaaag
     3721 gctcagggtg agaatgggcg gcaggtagcc cagcaccgtg tagcccatca ccatcagcac
     3781 ctcttgcggg ttcacgctgc cgcgggtggc ctgggccagc gtgcgtatca gtgtcatcgt
     3841 catgacgatg gtcaccagca ccaccagggt cgcgccgaaa ctgcgggcga gttccttgcg
     3901 cagggaggaa tggaataaca tcggcgcgtt tggcgcaaag cgacagaaaa aaagcgaatt
     3961 atggacttcg aactcaagac cctgggcctg gcaggcgcgg ccggcgcgaa atgcgatgcg
     4021 ctggtgctgc tggtcgggga aacgttcaag ccgggcaagg atgcgctgtc gaagctggcg
     4081 gcggatgcgc tcaaggcgcg cgacctcgag gccaagcccg gcaagctgct gcaggcttac
     4141 cgcaccaacg gcatcgcggc gccgcgggtc ttgctggcag gctgcggcga cggctcgggc
     4201 aagcgggttc acgccgccgt gcaagccgcc gtgaccagct tgcggacctc cggcgccaag
     4261 cggctggtca tctgcctgcc gcaggacgcc gacgaggatg ccgtgcgcgc cgccgtcctt
     4321 gccacggcgg aaacgagcta tgtgtatgtg acgaccaagt ccaagccgga aggccgcgaa
     4381 ttgcagcgcg tgaccgtcgc cgtcgcggac gcggcacggc atcggaccgg tttcgccgcg
     4441 gccgaggcgg ctgccaacgg tatcgagttc gcccgtgaac tgggcaacct gccgcccaac
     4501 ctcgccacgc cgacgcggct gggcgaggaa gcaagaaagt tggccaaggc tcatggcctc
     4561 cagtgcgagg tgctgggacc caaggaagtc gccaagatcg gcatggggtc gttcatggcg
     4621 gtggccaagg gatccgacca gccgctgcgc ttcatcgtgc tcaagtacca cggcgcggcc
     4681 caaagcgagg cgccggtggt gctggtgggc aagggcatca ccttcgacag cggcggcatc
     4741 tccatcaagc cctcgcagga catggacgag atgaagttcg atatgggcgg cgcggccagc
     4801 gtgctcggca cctttcacgc gattggcgaa ttgcgcccgg cgatcaacgt ggtcggcctg
     4861 atccccagtt gcgagaacat gcccgacggg cggtcggtca agccgggcga catcgtcgcc
     4921 agcctgagcg ggcaaaccat cgaaatcctc aacacggacg ccgaaggacg gctgatcctt
     4981 tgcgacgcgc tcacctatgc cgagcggttc aagccgcggg cggtgatcga catcgcgacg
     5041 ctcaccggtg cctgcgtggt cgcgctgggc ggcgtgcgca gcggcctgtt cgccaccgat
     5101 gaaaccctgg cccaggccct gctggacgcc ggcgacgcgg cgctggatcc gtgctggcgc
     5161 atgccgctgg acgatgaata tgcggacgga ttgaagagca acttcgccga cgtggccaac
     5221 gtggcggggc gttccggcgg cgccgtcaac gcggccaagt tcctgcagcg ttttgcgggc
     5281 aactatccct gggcgcacct ggacatcgcg ggcaccgcct ggaagagcgg tgcggccaag
     5341 ggttccaccg gccgcccggt cggcctgctg gtgcaattcc tgctgggcca gcagagcgcc
     5401 acgcccaagc cggctcgaaa aggcggcaag cgcaaggcgg catgaacgca tgaaatgtgc
     5461 atttgtcatc ccgggctggg ctcgcggaac gcccggggcg cgcaagctgt ccagtgcagc
     5521 gagcccaatg ccgggcctac gctatgcacg tgccgaggca tcacgctccg gcccggcccc
     5581 gggatccatg gattcggggt catggattgc gggtcaagcc cgcaatgaca actccctctc
     5641 ttgtcatgcc gggcccgacc cggcatccat gaattcggac ttcatggatt gcgggtcaag
     5701 cgatgacaat ccttagcccc tagccccttc cgccaatgac cgaggtcacc ttccatttca
     5761 acgcgccgga caaggtgggc catgcctgcc ggctgctgcg caaggcggtg gccagtggag
     5821 cgaaggtggt ggtgaccggc gacgccgtgc tgttgcgcga gctcgacgtc gccctctgga
     5881 ccttcgcgcc ccttgaattc gtgccccatt gcctgagcgg cgccagtgcg ccggcggtgc
     5941 tggcggcttc ccccgtggtg ctggcggatt cggcccggtc ggccccgcac cagcatgtgc
     6001 tgctcaacct gggagccggc gtgcctgagg gcttcgagcg tttcgagcgc ctgatcgagg
     6061 tcgtgaccgg gcaggaagac gaccggcagc agggacgccg gcggtggaag cattacgccg
     6121 accgcggcta cgccatcaag ggcgaggacc ttggcgcgaa ggagactcgc tgatggccaa
     6181 tcgcaagccg ccgcgctatg tgccgacact gacggaagtg gtcgggactg ccggtgaatc
     6241 caagcttgcg gaaagcgccg gcatttcgca ggagcagatc atccaacggg tgatgcagcg
     6301 aatcgacctg acgctcgagc gccgtctgcg ggaggcgatc gcggccacgg tgctcgagca
     6361 gacccgctcg
//
LOCUS       DASWIY010000403         5298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1025661, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000403 DASWIY010000000
VERSION     DASWIY010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025661"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VGA59_14115"
     CDS             complement(<1..845)
                     /locus_tag="VGA59_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX9720855.1"
                     /translation="MLNIFTLANGRLFQEEIESLEELSKFQPIWVDLEAPTLEEKRWI
                     KQYFGLSIPEDAMDEDIEESARFYEEDNGDLHIRSDFLIADDEEPRTVRVAFILNLVN
                     DSLKSKGVLFSIHDEDVPVFRLLRLRARRAPGLIEDAKEVLLKLFDADAEYSADTLEG
                     IYDELEKVSTQVLAGEVTDALAGEVLGQIARQEDLNGRIRRNVMDTRRAVSFMMRSRM
                     LSAGQFEEARQILRDIESLDNHTAFLFDKINFLMDATVGFININQNKIIKIFSVAAVA
                     LLPPTL"
     gene            complement(1131..2330)
                     /locus_tag="VGA59_14120"
     CDS             complement(1131..2330)
                     /locus_tag="VGA59_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9720856.1"
                     /translation="MSLSTIINAGLTRPSRPIHALVLMGGGARTAYQVGVLQAMAAML
                     ELQGERVMGFPFQVLVGTSAGALNVAYLAGAATHGLQSFGELRRFWSRLRSVDVYELN
                     VSRWVRFSRLVAALSLWRSARGNGAILNTMRLVDTLHHAISLQGIEDSLQTHAIDAVA
                     VTASSYTSGVHWTFCHTRHDRVDSWNRPGARSEFQPLTIEHLMASSAIPFLFPATPLW
                     VDGRREYFGDGSMRQISPLSPALHLGAHRILVVGVGQPQRSGLVGGDPREAPARGPTL
                     GGMAGHALASVFHDTLQADVEQAQRVTRTLQQLPREVAAVLPYRSVEVLSLQPSQSLD
                     AIAQAHAHELPGSVRRALGGLGALKGGGGALASYLLFEPGFVQALVTLGEHDAYAHKE
                     ELLAFFD"
     gene            2653..2853
                     /locus_tag="VGA59_14125"
     CDS             2653..2853
                     /locus_tag="VGA59_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720857.1"
                     /translation="MIQLATDPTASRTSYRPAAQGEERAKESTFETKRGRTGHAWPFL
                     SRYAAQPARLDNRETPRTDSGS"
     gene            2881..3051
                     /locus_tag="VGA59_14130"
     CDS             2881..3051
                     /locus_tag="VGA59_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720858.1"
                     /translation="MSTERKSPNKFADTPRDRPVSEHTEHANLERPGAQAHPRARDWS
                     HFSSPVKAWRLH"
     gene            3328..3657
                     /gene="raiA"
                     /locus_tag="VGA59_14135"
     CDS             3328..3657
                     /gene="raiA"
                     /locus_tag="VGA59_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX9720859.1"
                     /translation="MNLTISGHHLEVTQALRNYVTTKLDRITRHFDQVVDIKVLLTIE
                     KQKEKERRQRAECKIHVKGNDIFAESSHEDLYAALDELVDKLDRQVGRHKTRLQDHHH
                     DAPKRVM"
     gene            3782..4249
                     /locus_tag="VGA59_14140"
     CDS             3782..4249
                     /locus_tag="VGA59_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX9720860.1"
                     /translation="MNRLASILPAAQVLVSVDATSKKRAFEEAGLLFENLHGLSRALI
                     TDSLFARERLGSTGLGHGVAIPHGRIKGLKSPMAALFRLAQPIGFDAPDEQPVGLLIF
                     LLVPEAATQKHLEILSEIAELLSDAPLREKIKASTDAAELHRLIATWQSAQPA"
     gene            4264..5214
                     /gene="hprK"
                     /locus_tag="VGA59_14145"
     CDS             4264..5214
                     /gene="hprK"
                     /locus_tag="VGA59_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX9720861.1"
                     /translation="MKPTVVSADVLFEDHRAQLKWEWVAGLGASERRFDEVAVRAARS
                     GADLVGYLNYIHPYRVQILGQREIAYLTNATSDDCARRISRIVTLEPPVLVLTDSQVA
                     PDALLSMCERAQIPMFATQEPSAFVIDVLRAYLSKHFAERTSMHGVFMDILGLGVMIT
                     GESGLGKSELGLELISRGNGLVADDAVDLFRINQTAIEGRCPELLQNLLEVRGIGLLD
                     IRAIFGETAVRRKMRLQLIVHLVRRDTMEREYERLPYEPLTQDVLGVPVRKAVIQVVA
                     GRNIAVLVEAAVRNTILQMRGVDTYQEFVERHRRAMEQDK"
BASE COUNT          995 a         1732 c         1670 g          901 t
ORIGIN      
        1 agcgtcggcg gcagcagcgc aacggccgcc accgagaaaa tcttgatgat cttgttctgg
       61 ttgatgttga tgaagcccac ggtagcgtcc atcaagaagt tgatcttgtc gaacaggaag
      121 gccgtgtggt tgtccagcga ctcgatgtcg cgcaggatct ggcgcgcctc ctcgaactgc
      181 cccgcgctca gcatgcggct gcgcatcatg aagctcacgg cgcgccgcgt gtccatcacg
      241 ttgcggcgga tgcgcccgtt caagtcttcc tggcgcgcga tctggccgag cacctcgccg
      301 gccagggcgt cggtcacctc gccggccagc acctgggtgc tgactttttc cagctcgtcg
      361 tagatgccct ccagtgtgtc ggcggaatac tcggcgtccg catcgaaaag tttcagcagc
      421 acctccttgg cgtcctcgat caatcccggc gcgcgccgcg cgcgcaggcg cagcagccgg
      481 aataccggga cgtcctcgtc gtggatcgag aacagcacgc ccttgctctt gagcgagtcg
      541 ttcaccaggt tcaggatgaa cgccacgcgg accgttcgcg gctcctcgtc gtccgcgatc
      601 agaaagtcgc tgcggatgtg caggtctccg ttgtcctctt catagaagcg cgccgactcc
      661 tcgatgtcct cgtccatcgc gtcctcggga atcgacaagc cgaagtattg cttgatccaa
      721 cgcttttctt ccagggtggg cgcctcgaga tccacccaga tcggctggaa tttggagagc
      781 tcctccagcg actcgatctc ctcctggaag agccggccgt ttgcgagcgt gaagatgttg
      841 agcatggctg gtcaaattgg ggtcagattc caattattcg gatggttaat gcgtggcgca
      901 cagcagcttt cgaataattg gaatgaactg tcgcagcgac acgcccgtgg cacgtgcttg
      961 gacaaaacag ttcaacgatc gccttcgcgg tgcggctact gcaggcactt gcgataccgc
     1021 gatcacctca atttcttcga aagctaccga ccagggtagt tttccaagga gcgctctccg
     1081 aaagtttcga ctcgacaatt atgccgtggc gtgggcttgc ccagcgccac ctaatcgaag
     1141 aacgccagca attcctcctt gtgcgcgtag gcatcgtgtt cgcccagggt caccagcgcc
     1201 tgcacgaagc ccggttcgaa cagcaggtag cttgcgaggg cgccgccgcc ccccttgagc
     1261 gcgccgaggc cgcccaatgc gcgccgcacc gagccgggga gttcatgggc gtgggcctgg
     1321 gcgatcgcat cgagcgactg gctcggctga agcgaaagca cttccacgct gcgatagggc
     1381 agcacggcgg ccacctctcg cggcaactgc tgcagggtgc gggtcacccg ctgggcctgc
     1441 tccacgtcgg cctgcagcgt atcgtggaaa acgctggcca gggcgtgacc ggccatgccg
     1501 ccgagcgtgg gtcctcgcgc cggcgcctcc cgcgggtcgc cgccgaccag gccggagcgc
     1561 tgcggctggc cgacccccac caccagaatc ctgtgcgcgc ccaggtgcag cgccggcgac
     1621 agcggcgaga tctggcgcat cgagccgtcg ccgaagtatt cgcgccgccc gtccacccac
     1681 aggggcgtcg ccgggaacag gaacgggatg gcgctggaag ccatcagatg ctcgatcgtg
     1741 agcggctgga actcggaacg cgcgcccggc cggttccagg aatccacgcg atcgtggcgg
     1801 gtgtggcaga aggtccagtg aaccccgctg gtatagctcg atgccgtcac cgccacggcg
     1861 tcgatggcgt gggtctggag cgagtcctcg atgccctgca gcgaaatcgc gtgatgcaag
     1921 gtgtccacca ggcgcatggt gttgaggatg gcgccgttgc cccgggcgct gcgccacaag
     1981 ctcagcgccg ccaccagccg gctgaagcgc acccatcggg agacgttcag ttcatagacg
     2041 tcgaccgatc gaagccggct ccagaagcgc cgcaactcgc cgaaggattg caacccgtgc
     2101 gttgccgcgc cggccaggta ggccacgttg agggcgccgg cggaagtgcc caccagtacc
     2161 tggaagggaa atcccatgac gcgctcaccc tggagctcga gcatcgcggc catggcttgc
     2221 agcacaccga cctggtacgc agtgcgggcg ccgccgccca tcagcaccag cgcgtggatc
     2281 ggccggctgg ggcgagtcaa tcccgcgttg atgatggtgc taaggctcat gggccgtcgg
     2341 tgcgcggcgg caggtgctgc cacgctctcc tctgaggcca tggtagagga agacgccgcg
     2401 cttggatacg gcgagcgctt cagccggcgg cccggctttg gcccttgcct taccggcccg
     2461 cgtgcgtgat cacgctgatc tcgccatcgc tctccgggct catcaggccg ccgagccgtc
     2521 cagtccggac catgaggcag gacgtgtcct acaaagctca actgatctgt ccgacatatc
     2581 ggatgaggca cggctcctac attcgatttg actatcccgg ccccaacccg tcgggaacgc
     2641 aaggagttat ccatgatcca gctcgcgacc gatccgaccg caagccgaac gagctaccgt
     2701 cccgccgccc agggcgaaga gcgggcgaaa gaatcgacgt tcgagaccaa gcgcggccgt
     2761 accggtcatg cctggccttt cctcagtcgt tacgcggccc agcctgcccg ccttgacaac
     2821 cgggaaacac cccggacgga cagcggtagc tgatcgcggc ccatttacgg agtgacatcc
     2881 atgtccacag aacgcaaatc cccgaacaag tttgccgaca cgccgcgcga caggcccgtg
     2941 agcgaacaca ccgaacacgc caatctcgag cggccgggcg cgcaagccca cccgcgcgcc
     3001 agggactgga gccatttttc cagcccggtg aaagcgtggc gccttcattg agccaccaca
     3061 acccgccaag atgatggagg cggccctcga agggccgctt ttttttggcc gggataaact
     3121 gctccggcaa aaacccagcg ttttcaatta cttggaaatc agtccgacaa agggtttcac
     3181 ttagccgggc ggctgcgacg aaatccattg ctttccgaag gtatcgcggg catagtgaat
     3241 tcaaccttca agaaaatcca ggttcgccgg ccgtccgggc gtcgcggacg gtcctttcaa
     3301 ccagagtgca gcaacaggag gctactcatg aatttgacga tcagcggtca ccatctcgaa
     3361 gttacccagg ccctgcgcaa ttacgtgacc accaagctcg atcgaatcac ccggcacttt
     3421 gaccaggtcg ttgatatcaa ggtgctactc accatcgaaa agcagaagga aaaggagcga
     3481 agacagcgcg ccgaatgcaa gattcatgtg aagggcaacg acatattcgc ggaaagttca
     3541 cacgaggacc tgtatgccgc cctggacgag ctggtcgaca aactggaccg gcaggtcggc
     3601 aggcacaaga cacgcctgca ggaccatcat cacgacgcac ccaaacgggt gatgtgaagt
     3661 aaaccaggag taaacgaggc gcccgacggg cgcctctttc ttttgggctg tcgacggatg
     3721 agccgtgccg cgcgtttgat gcggggtacc ctccgcagtg cataattcgt acacaccgac
     3781 catgaatcga cttgcgtcca tcctgccagc cgctcaagtg ctcgtaagcg tcgatgccac
     3841 aagcaagaag cgcgctttcg aagaagccgg attgctgttc gaaaacctgc acggcctgtc
     3901 gcgcgcgctg atcaccgaca gcctgtttgc gcgcgagcgc ctgggctcga cgggcctggg
     3961 tcatggtgtg gccattccgc acgggcgcat caagggactg aaatcgccga tggccgcgct
     4021 gttccggctg gcccagccga tcggcttcga tgccccggat gagcagccgg tcggcctgct
     4081 gatcttcctg ctggtgcccg aggcggcgac ccagaagcac ctggaaatcc tgtcggaaat
     4141 tgccgagctg ctcagcgacg cccccttgcg cgagaagatc aaggccagca ccgatgcggc
     4201 cgagctgcac cggctgattg ccacctggca gtcggcacag cccgcctaga gcgcccagcg
     4261 cctgtgaaac ccaccgtcgt cagcgccgac gtcctgttcg aagaccaccg cgcccagctc
     4321 aaatgggaat gggtggccgg gctgggggcg tcggagcgcc gcttcgacga agtcgccgtg
     4381 cgcgccgccc gctcgggtgc cgacctggtg ggctacctca actacatcca cccctaccgc
     4441 gtccagatcc tggggcagcg ggaaatcgcc tacctgacca acgcgacttc cgacgactgc
     4501 gcccggcgca tctcccgcat cgtcaccctg gagccgccgg tgctggtgct gaccgacagc
     4561 caggtggcgc ccgacgcgct gctgtcgatg tgcgagcggg cccagattcc gatgttcgcg
     4621 acgcaggagc cgtccgcctt cgtgatcgac gtgctgcgcg cctacctgtc caagcatttc
     4681 gccgagcgca cctcgatgca cggcgtgttt atggacattc tggggcttgg ggtgatgatc
     4741 acgggcgagt cgggcctggg caagagcgaa ctgggcctgg agctgatctc gcgcggcaac
     4801 ggcctggtgg ccgacgacgc cgtcgacctg ttccggatca accagaccgc gatcgaaggg
     4861 cgctgccccg agctgctgca gaacctgctg gaagtgcgcg gcatcggcct gctggacatc
     4921 cgcgccatct tcggcgagac cgccgtgcgg cgcaagatgc gtttgcagct catcgttcac
     4981 ctggtgcgca gggacacgat ggagcgcgag tacgagcggc tgccctacga gccgctcacc
     5041 caggacgtgc tgggggtgcc ggtgcgcaag gccgtgatcc aggtggtggc cggccgcaac
     5101 atcgccgtgc tggtcgaggc cgcggtgcgc aacaccatcc tgcaaatgcg cggcgtcgac
     5161 acgtaccagg agttcgtgga gcgccatcgc agggcgatgg agcaggacaa gtgacgcccg
     5221 cagcgcgtgc gtcatcccgg gcttgacccg ggattcatag cggctcacgc agagcgccgc
     5281 tccagtgaca tggatgcc
//
LOCUS       DASWIY010000404         7900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1384486, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000404 DASWIY010000000
VERSION     DASWIY010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384486"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..713)
                     /gene="hutC"
                     /locus_tag="VGA59_14150"
     CDS             complement(3..713)
                     /gene="hutC"
                     /locus_tag="VGA59_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HEX9720862.1"
                     /translation="MMRSELPAYEQVKAWVKRHIASGEWKPGDPVPSEAAFMQQFGVS
                     RMTVNRALRELAAEGLVTRVQGSGTRVAQLHRISSRLTIRDIHEEVVERGHEHTTRVL
                     QAGKEKARAELALSLGLRTGTTAFHTVLVHYENGVPIQYEDRYVNPAAAPRYLETDFT
                     RTSPTLHLLQHAPLTEASYSIEACLPTAGEARELSIKRSEPCLVMMRRTVSGGHVASV
                     ARLVYPGTRYSFAGQFQA"
     gene            935..2659
                     /gene="hutU"
                     /locus_tag="VGA59_14155"
     CDS             935..2659
                     /gene="hutU"
                     /locus_tag="VGA59_14155"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX9720863.1"
                     /translation="MSDLSQTPFLDSKARPVQAPRGTQLACANWQIEAAYRMIQNNLD
                     PAVAENPDELVVYGGIGKAARNWPCFDAILDSLRKLQPDETLLVQSGKPVGVFRTHTG
                     APRVLIANSNLVPKWATWEHFNELDRAGLMMFGQMTAGSWIYIGTQGIVQGTYETFAE
                     AGRQHYNGDLAGRWVLTAGLGGMGGAQPLAASFAGACSLNIECQQTRIDFRLRTRYLD
                     KQAKDLDEALQLIAHHTSRNEAVSIGLCGNAAEIVPELARRAKAGGIKPDIVTDQTSA
                     HDIINGYLPIGWAVAQWQAAQKDPAQHARLTKAAGESCAVHVRAMLEFHAMGVPTVDY
                     GNNLRQVAKDFGVANAFDYPGFVPAYIRPLFCRGTGPFRWVALSGDPEDIRKTDAKMK
                     ELFPQDAHLHRWIDMAQQRISYQGLPARICWIGLGERHRAGLAFNEMVKSGELRAPIV
                     IGRDHLDSGSVASPNRETEAMKDGSDAVSDWPLLNALLNTASGATWVSLHHGGGVGMG
                     YSQHAGVVIVCDGTAEAAEKIERVLWNDPATGVMRHADAGYDIAQECARDNGLKLPML
                     QTTSLSGR"
     gene            2656..4227
                     /gene="hutH"
                     /locus_tag="VGA59_14160"
     CDS             2656..4227
                     /gene="hutH"
                     /locus_tag="VGA59_14160"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX9720864.1"
                     /translation="MKSTAKETLVPGELSLAQLRRLCGSERAAVEIDPACRPRVRASA
                     ELVRRATEGEAPVYGVNTGFGKLATTRIESEDLAFLQLKLLRSHAVGVGDPLPERVVR
                     LILLLKAASLARGFSGVREEVIDTLLALHNRGVTPVIPCQGSVGASGDLAPLAHLSLP
                     LVGEGEVFYQGERLPAADALERAGIDAIQLSAKEGLALINGTQVSTALAIDALLACDR
                     LFEAAVISGALTLDAARGSDGPFDPRIHAVRGQPGQIDCAAAYRALMVGSDIRASHLE
                     DDDRVQDPYSLRCQPQVMGACLDQMRYSAQVLVREANAVTDNPLVFAGQADFRGGELV
                     SGGNFHAEPVALVADALAVAIAEIGAITERRIAMLVDTVVSRLPPFLTPEPGLNSGFM
                     IVHVTAAALASENKSLAHPASVDSLPTSANQEDHVSMATFAARRLQPMLRNTAHIVAI
                     ELLAAAQGIEFLRPMKSAPALEGVLRLVRSVSPAMMQDRSLARDIEAVHHLVAAGDIG
                     HATEAHIPELHALRL"
     gene            4410..5354
                     /locus_tag="VGA59_14165"
     CDS             4410..5354
                     /locus_tag="VGA59_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9720865.1"
                     /translation="MKKLLALSLLALACTAGQAQDTKVAVAISGWTGFAPLTLAKEAG
                     IFKKNGLDVSIKKIPQKDRHLAIASGDVQCAATTVETWVVWNANGVATTQIFQLDKSY
                     GADGMVVKPHITKITDLKGKTVAASAPGTSPYFALAWFLKKNGLSTKDVKVVNLEPQA
                     AANAMIAGSTDIDAAMTYEPYLSAVRAKPEAGKIIATTLDYPMVMDTFGCTPKFLNEN
                     PKAAKALTDSYFEAVEMVKRDPKKSFEIMGADVKQSGEAFAKSAAYLRWQDREANLKF
                     FSGEHAAFSKEAADLLMEAGTIKQLPDMSKLADTRFLK"
     gene            5366..6145
                     /locus_tag="VGA59_14170"
     CDS             5366..6145
                     /locus_tag="VGA59_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9720866.1"
                     /translation="MRPLQSVNPGARVVLGIAFFILFFLVWAAATFGGFVSKTFLADP
                     VTMVRSGYDLLANQGFAFDIGMTVWRVLGGFAIAAVLAVPLGVLMGAYKPVEAFFEPF
                     VSFARYLPASAFIPLLILWAGIGEAQKLAVIFIGSFFQLVLMIAVSVGNTRRDLVEAA
                     YTLGAGDRGIVTRVLMPSSAPEIAETLRMVLGWAWTYVIVAELIGASSGIGHMITDSQ
                     ALLATDQIIFGIIVIGLIGLVTDFAFKAFNRKLFPWAQLGR"
     gene            6237..7028
                     /locus_tag="VGA59_14175"
     CDS             6237..7028
                     /locus_tag="VGA59_14175"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720867.1"
                     /translation="MSILEITRVAKTFPGANGGTVALQATDLSVQENDFITVLGPSGC
                     GKSTLLRMVAGLDTPTTGSIELDGRPVLGPGADRGMVFQSYTLFPWLTVLQNVCFGLR
                     EKGMPLSDQHDLARQFIGKVGLEGFESHFPKQLSGGMQQRTALARALANNPRILLMDE
                     PFGALDHQTRELMQELLEGIWEAERKTVLFVTHDIDEAIFMANRVVVMSARPGRIKCD
                     LPVAIDHPRHYSVKTKPVFSDLKARLTEEIRVEVQRAIGMLALAA"
     gene            7053..7523
                     /locus_tag="VGA59_14180"
     CDS             7053..7523
                     /locus_tag="VGA59_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9720868.1"
                     /translation="MKKSAGLLMYRRSDDGTVQVLLAHPGGPFWSGKDEGAWTIPKGG
                     YEAPEEPLAAAQREFTEETGFAVTPPFVPLGEITQKSGKRITAWAFAGDCDPAQICGN
                     TFELEWPPRSGRRQNYPEIDRIEWFTLDEARRKIIPAQRALLDRLEQTLSPGSV"
     gene            7587..>7900
                     /locus_tag="VGA59_14185"
     CDS             7587..>7900
                     /locus_tag="VGA59_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX9720869.1"
                     /translation="MTRLACDIVSPAGGRRASPGLPALAIAALVAGCGPNAPPAWQGY
                     VEGEYVMVAAPAAGRLERRFVDRGAQVQAGAPLFVLEQENEKAARQEAQERLNNAQAR
                     LA"
BASE COUNT         1394 a         2630 c         2612 g         1264 t
ORIGIN      
        1 gctcatgcct gaaactgccc cgcgaagctg tagcgcgtgc cgggatagac cagccgcgcg
       61 acgctggcga catggccacc gctgaccgtg cgccgcatca tcaccaggca gggctcgctg
      121 cgcttgatgc tcagttcgcg cgcttcaccg gccgtgggga gacaggcctc gatcgagtag
      181 ctggcctcgg tcagcggcgc gtgctgcaac aggtgcaggg ttggcgaggt gcgggtgaag
      241 tcggtttcca ggtaccgggg cgccgcagcc gggttcacgt agcggtcttc atattgaatg
      301 gggacgccgt tttcgtaatg gacgagcacg gtatgaaagg cggtagtgcc ggtgcgcagc
      361 cccaacgaca gcgccagttc ggcgcgagcc ttctccttgc cggcctgcaa tacgcgcgtg
      421 gtgtgctcat ggccgcgctc gaccacctcc tcgtggatat cgcggatggt gagcctggac
      481 gatatccggt gcagctgggc cacgcgggta ccggagccct gcacccgcgt caccagcccc
      541 tcggccgcga gttcgcgcag ggcccggttc acggtcatcc ggctgacgcc gaactgctgc
      601 atgaaggcgg cctcgctggg caccgggtcg cccggcttcc actcgccgct ggcgatgtgc
      661 cgcttgaccc aggccttgac ctgttcgtag gcgggtagct cgcttcgcat gatgcggcaa
      721 ggataaccca gttgacacaa gttgtataga caactacgat gccaccgaat cggacagcct
      781 ggatgccccc ggatcaagtc cggggcaggc tcgtcaagcc cggcatgaca gaagagtttg
      841 tcattgcggg catggccagg cttgacccgc aatccatgac ccccgattca tggacgccgg
      901 accgagcccg gcatgacaga agtaaggagc agtgatgtcg gatctctcgc aaacaccttt
      961 cctggacagc aaggcacggc cggtgcaggc gccgcgcgga actcagctgg cctgcgccaa
     1021 ctggcagatc gaagcggcct accgcatgat ccagaacaac cttgaccctg cggtggccga
     1081 aaacccggat gaactcgtgg tctatggcgg catcggcaag gcggcgcgca actggccctg
     1141 cttcgatgcg atcctggaca gcctgcgcaa gctccagccc gacgagacgt tgctcgtgca
     1201 gtcgggcaag ccggtcggcg tcttccgcac ccacaccggc gccccgcgcg tgctgatcgc
     1261 caattccaac ctggtgccca agtgggcgac ttgggaacac ttcaacgagc tggaccgcgc
     1321 cggcctgatg atgttcggcc agatgaccgc gggcagctgg atctacatcg gcacccaggg
     1381 catcgtgcag gggacttacg agaccttcgc agaagccggt cggcagcact acaacggcga
     1441 cctcgccgga cgctgggtgc tcactgcggg gcttggcggc atgggcggag cccagccgct
     1501 ggccgccagt ttcgccggcg cctgctcgct caatatcgag tgccagcaga cccgcatcga
     1561 tttccgcctg cgcaccaggt atctggacaa gcaggccaag gacctggacg aggcgctgca
     1621 gctcatcgcg caccatacct cgcgcaacga agccgtgtcg atcgggctgt gcggcaacgc
     1681 ggccgagatc gtgccggaac tggcaaggcg cgccaaggcc ggtggcatca agccggacat
     1741 cgtcaccgac cagacctcgg cccacgacat catcaacggc tacctgccga tcggctgggc
     1801 cgtggcgcaa tggcaggcgg cgcagaaaga tccggcgcaa cacgcgcggt tgaccaaggc
     1861 agcgggcgag tcctgcgcgg tgcatgtcag ggccatgctg gagttccatg ccatgggcgt
     1921 gcccacggtg gactacggca acaacctgcg ccaagtggcc aaggacttcg gcgtggccaa
     1981 cgcgttcgac tatcccggct tcgtgcccgc ctatatccgg ccgctgttct gccgcggcac
     2041 cgggccgttc cgctgggtcg cgctgtccgg cgatccggag gacattcgca agaccgacgc
     2101 gaaaatgaag gagctgtttc cgcaagacgc ccacctgcac cggtggatcg acatggcgca
     2161 gcagcgcatc agctaccagg gtctgccggc gcgcatctgc tggatcggcc tgggggagcg
     2221 gcaccgggcg ggactggcct tcaacgagat ggtgaagagc ggcgaactca gggcgccgat
     2281 cgtgatcggg cgcgatcacc tggacagcgg ctcggtggct tcccccaacc gcgaaacaga
     2341 ggccatgaaa gacggtagcg acgcggtgag cgactggccg ctgctcaacg ccctgctcaa
     2401 caccgccagc ggcgccacct gggtcagcct gcaccacggc ggcggcgtgg gtatgggcta
     2461 ctcgcagcat gccggggtcg tgatcgtgtg cgacggcacg gccgaggcgg ccgaaaagat
     2521 cgaacgcgtg ctgtggaacg acccggccac cggcgtgatg cgacatgccg acgcgggcta
     2581 cgacatcgca caggaatgcg cccgcgacaa cggcttgaaa ctgccgatgc tgcaaaccac
     2641 ctccctctcc ggccgatgaa atcgaccgcg aaagaaaccc tcgtccccgg cgaactctcg
     2701 ctggcgcagc tgcggcggct gtgcggcagc gagcgcgccg cggtcgagat cgatccggca
     2761 tgccgcccac gcgtgcgtgc cagcgctgaa ctggtgcggc gcgccaccga gggagaagcc
     2821 cccgtctatg gcgtcaacac cggcttcggc aaattggcca cgacgcggat cgaaagtgag
     2881 gatctcgcct tcctgcagct caagctgctg aggtcgcatg ccgtgggcgt cggcgatccg
     2941 ctgcccgagc gggtcgtgcg cttgatcctg ctgctcaagg ccgcgagcct ggcgcgcggc
     3001 ttttcaggcg tgcgcgaaga agtgatcgac acgctccttg ccctgcacaa caggggcgtc
     3061 acgcccgtca ttccctgcca gggttcggtg ggcgcgtccg gcgatctggc cccgctggcg
     3121 catctgtcgc tgcctctggt cggcgagggc gaagtcttct accagggcga acggctgcct
     3181 gcggccgatg cgctggagcg cgccggcatt gatgcgatcc agctctcggc aaaggaaggc
     3241 ctggccctga tcaacggcac acaggtctcg acggcattgg ccatcgacgc gctgctggct
     3301 tgcgaccggt tgttcgaagc ggccgtgata tcgggtgcac tcacgctcga cgcggcacgc
     3361 ggcagcgacg ggccgttcga cccgcggatc cacgccgtgc gcggccagcc ggggcagatc
     3421 gattgcgccg ccgcctaccg cgcgctcatg gtgggcagcg acatcagggc gtcgcacctg
     3481 gaagacgacg accgagtgca ggacccgtac agcctgcgct gccagcccca ggtcatgggc
     3541 gcttgcctgg accagatgcg ttattcggcc caggtgctgg tgcgcgaggc caatgccgtc
     3601 accgacaacc cgctggtgtt cgcgggacag gccgacttcc gcggggggga gcttgtctcc
     3661 ggcggcaact tccatgccga gccggtggca ctggtcgccg acgccctggc cgtcgccatc
     3721 gccgagatcg gcgcgatcac cgagcggcga atcgcgatgc tcgtggacac tgtggtgtca
     3781 cggttgccgc ccttcctcac gcccgagccg ggcctgaact cgggcttcat gatcgtgcac
     3841 gtgaccgcgg cggcactcgc gtcggaaaac aagtcgctcg cccatccggc cagcgtcgac
     3901 agcctgccga cctcggccaa ccaggaagac catgtcagca tggctacctt cgcggctcgg
     3961 cggctgcagc ccatgctgcg caacacggcg cacatcgtcg ccatcgagtt gctggcagcg
     4021 gcgcagggca tcgagttcct caggccgatg aaaagcgcgc cggccctgga aggcgtgctg
     4081 cgcctggtgc gcagcgtttc gcccgcgatg atgcaggacc gcagcctggc gcgcgacatc
     4141 gaggcggtgc accacctggt ggcggcgggc gacatcggac acgccaccga agcccatatc
     4201 ccggaattac acgcattgcg attgtgagca acccaccccc gaagcggcct acggccgatg
     4261 ccccctcggg ctttgccctc tccccctcaa gggggcgcca ccaacggccc ggcaaagccg
     4321 gttccgtggt ggcccgcgaa ttgctcgtcc tgtttccaag cgcgcgtgcg gcgcgtagta
     4381 tctttactca accatccacg gagaacctca tgaagaagct gctcgccctc tcgctgctgg
     4441 ccctggcctg cactgccggc caggcccagg acaccaaggt cgccgtcgcg atctcgggct
     4501 ggaccggatt cgcgccgctg acgctggcca aggaagccgg catcttcaag aagaacggcc
     4561 tggacgtcag catcaagaag attccgcaga aggaccgcca cctggccatc gcctcgggcg
     4621 acgttcaatg cgccgcgacc accgtggaga cctgggtggt gtggaacgcc aacggcgtgg
     4681 cgaccacgca gatcttccag ctggacaaga gctatggcgc cgacggcatg gtggtcaagc
     4741 cccatatcac caagatcacg gacctgaagg gcaagaccgt ggccgccagc gcgccgggta
     4801 cttcgcccta cttcgcgctg gcctggttcc tgaagaaaaa cggcctgtcg accaaggacg
     4861 tcaaggtcgt gaacctcgag ccgcaggcgg ccgccaacgc catgatcgcg ggcagtaccg
     4921 acatcgacgc ggccatgacc tatgagcctt atctttccgc cgtgcgcgcc aagcccgagg
     4981 ccggcaagat catcgcgacc acgctggact atccgatggt gatggacacc ttcggctgca
     5041 cgcccaagtt cctgaacgag aatcccaagg cggcgaaggc gctgaccgac agctatttcg
     5101 aagcggtgga gatggtcaag cgcgacccga agaagagctt cgagatcatg ggcgccgacg
     5161 tgaagcaaag cggcgaagcc ttcgcgaaat ccgcggccta cctgcgctgg caggaccgcg
     5221 aagcgaacct caagttcttc tcgggcgagc acgccgcctt cagcaaggaa gcggccgatc
     5281 tgctgatgga ggccggcacc atcaaacagc tgcccgacat gagcaagctg gccgacacgc
     5341 gcttcctcaa gtaagcccgc agccagtgcg tccgctgcaa tccgtcaacc ccggcgcccg
     5401 tgtcgtgctg gggattgcgt ttttcatcct gttcttcctc gtgtgggcgg ccgcgacatt
     5461 cgggggcttc gtctccaaga cgttcctggc cgatccggtc acgatggtga gatcgggcta
     5521 cgacctgctg gcgaaccagg gcttcgcctt cgacatcggc atgaccgtct ggcgggtgct
     5581 cggcggtttt gcgattgccg cggtgctggc cgttcccctg ggggtgctca tgggcgcgta
     5641 caagccggtc gaggctttct tcgagccgtt cgtgagcttt gcgcgctacc tgccggcctc
     5701 ggccttcatt ccgctgctga tcctgtgggc cggcatcggc gaggcccaga agctggcggt
     5761 catcttcatc ggctctttct tccagctggt gctgatgatc gccgtcagcg tgggcaatac
     5821 gcgccgcgac ctggtcgagg cggcctacac gctgggcgcg ggcgaccgcg gcatcgtcac
     5881 gcgcgtgctc atgcccagca gcgccccaga gatcgccgaa acgctgcgca tggtgctggg
     5941 ctgggcctgg acctatgtga tcgtggcgga gctgatcggc gcttccagcg gcatcggcca
     6001 catgatcacc gacagccagg cgctgctggc gaccgaccag atcatcttcg gcatcatcgt
     6061 gatcggcttg atcggcctgg tcaccgattt cgccttcaag gccttcaacc ggaagctgtt
     6121 tccatgggca cagctggggc gatgactgtc attgcgggct gggctcgcgg atggatcccg
     6181 gatcaagtcc gggatgacaa gtgcgcgcgc gaatcaagcc tgcaacgaca agtcgcatga
     6241 gcattctcga aatcacgcgc gtcgccaaga cgtttccggg ggccaacggc ggcaccgtgg
     6301 cgctgcaagc cacagacctg tcggtgcagg agaacgactt catcaccgtc ctcgggcctt
     6361 ccggctgcgg caagagcacg cttttgcgca tggtggcggg gctggatacg ccgaccaccg
     6421 gcagtatcga actcgacggc cggcccgtgc tgggccccgg cgccgatcgc ggcatggtgt
     6481 tccagagcta caccctcttc ccctggctca cggtgctgca gaacgtctgc ttcggcctgc
     6541 gcgagaaggg catgccgctg tccgatcagc atgacctggc ccggcagttc atcggcaagg
     6601 tggggctcga gggattcgag agccattttc ccaagcagct ttccggcggc atgcagcagc
     6661 gcaccgcgct cgcccgcgcc ctggccaaca atccgcgcat cctgctgatg gacgagccgt
     6721 tcggcgcgct ggaccaccag acccgcgagc tgatgcagga actgctggaa ggcatctggg
     6781 aagccgagcg caagacggtg ctcttcgtca cccacgacat cgacgaggcg atcttcatgg
     6841 ccaaccgcgt cgtggtgatg agcgcgcggc ctgggcgcat caagtgcgat ctgccggtag
     6901 ccatcgatca cccgcgccac tactcggtga aaacgaaacc ggtgttctcc gacctcaagg
     6961 cccggttgac cgaggaaatc cgcgtcgagg tccagcgcgc gatcggcatg ctcgcgcttg
     7021 cggcctagca aaccgcgtcg agcgccgcgg ccgtgaagaa aagcgccggt ttgttgatgt
     7081 accgccgcag cgacgatggc acggtgcagg tactgttggc gcatcccggc ggtccgttct
     7141 ggagcggcaa ggacgaaggc gcctggacga ttcccaaggg cgggtacgag gcacccgagg
     7201 aacccctggc cgcggcccag cgcgagttca ccgaagaaac ggggttcgct gtgacgcctc
     7261 ccttcgtgcc gctcggcgag atcacccaga aaagcggcaa acgcatcacg gcctgggcct
     7321 tcgcgggcga ctgcgacccg gcccaaattt gcggcaacac cttcgaactc gagtggccgc
     7381 cgcgttccgg gcgcaggcaa aactaccccg agatcgatcg catcgagtgg ttcacgctgg
     7441 acgaggcgcg gcgcaagatc atcccggccc agcgcgcttt gctcgatcgg ctggagcaga
     7501 cgttgtcccc cgggtccgtg tgagaagcgg tcgcgatctt gatgcgagac aacagcaagt
     7561 tcacagagaa ggttacagtc cgcggcatga cccgtcttgc ctgcgacatc gtttcaccgg
     7621 ccggtggacg aagggcgtcg cccggcctgc cagcgctcgc gatcgcggca ctggtcgccg
     7681 gctgcggtcc gaacgcaccc ccggcctggc agggctacgt cgagggtgaa tacgtgatgg
     7741 tcgccgcgcc ggcggcaggc cggctggaac ggcgcttcgt cgatcgcggc gcgcaggtcc
     7801 aggccggcgc accgctattc gtgctggaac aggaaaacga gaaggcggcc cgccaggagg
     7861 cgcaggagcg gctgaacaac gcgcaggccc ggctcgcgaa
//
LOCUS       DASWIY010000405         6083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2149341, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000405 DASWIY010000000
VERSION     DASWIY010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2149341"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1361)
                     /locus_tag="VGA59_14190"
     CDS             complement(87..1361)
                     /locus_tag="VGA59_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9720870.1"
                     /translation="MHHVILGAGPAGVIAAETIRKHQPQDVITIVGDEKEPPYSRMAI
                     PYLLMGNIPEAGTWLRKGLGHFDALQIKLVQGRATHIDIGTRSVALDSGQQLRFDTLL
                     IATGSTPIRPPIPGIDLPGVHPCWTLEDARKIMGLAVKGARVLQMGAGFIGCIIMEAL
                     AARGVHLSVVEMGDRMVPRMMGPTAGGMIKEWCEMKGVEVFTGTRVEAIDTATPLSVR
                     LSNGKRLTADLVISATGVRPAIGFLENSGIRCLLGVLTDEHMQTNVPGIYAAGDCAEA
                     FDKISGKMMVSAIQPNAADQARVAGLNMAGQKAVLPGVTQINVLDTLGLVSASFGDWQ
                     GVPGGDQVELTDRAAGRHLSLQFKGDVMVGCNSIGWTDHVGVLRGLVEGQVRLGPWKE
                     YLKQDPTRLMEAYLASAQARDHWQGAGDERRR"
     gene            complement(1365..3212)
                     /locus_tag="VGA59_14195"
     CDS             complement(1365..3212)
                     /locus_tag="VGA59_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX9720871.1"
                     /translation="MSWAGKILRVDLTKGTVTPEPLNMAWAQAYIGSRGLASKYLVEE
                     MDPKVDPLSPANKLIWSTGPLTGTMASTGGRYTVVTKGPLTGAIACSNSGGYFGAELK
                     MAGWDMVIVEGKSPTPVYLYINDDVAELRDASGLWGKTIWQTEELLKKTLQDPLVRVS
                     SIGRAGENQVLYASIVNDLHRAAGRSGVGAVAGSKNLKAIAVRGTKGVGNIKDPAAFM
                     KAVKEKKKILADNAVTGQGLPAFGTQVLMNVINEMGALPTRNHKDVQFEGAKDISAEA
                     MVAVRKTDGKKHLVTNQACFGCTIACGRISKIDEGHFTVANKPQYWGANGGLEYEAAW
                     ALGAANGVNDLEALQYANLLCNEDGIDPISFGATIGAVMELYQMGVLTKEQIGVEAPF
                     GSAQALAFLAEQTVTGQGFGKEIGQGSKRLTAKYGHPELSMSVKGQEFPAYDSRGIQG
                     MGLAYATSNRGACHLRGYTVASEVLGIPVKTDPLVSEGKPELVKAFQDATAAFDSSGL
                     CIFTTFAWGLADLAPQISAACGEDYNTEELGKIGERIWNMERDFNNRAGFTAADDNLP
                     PRLLTEPAQTGPAKGKVNELAKMLPKYYEVRGWNPDGTIKPETRQRLGL"
     gene            complement(3226..3699)
                     /locus_tag="VGA59_14200"
     CDS             complement(3226..3699)
                     /locus_tag="VGA59_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9720872.1"
                     /translation="MQKVLHINADKCTGCLQCEMACSFENYGTFATAKSRIKVFDFHH
                     TGKKVPYTCTQCDEAWCLHACPVEAITIDRLTGAKVVNDSTCVGCKVCTIACPFGTVN
                     YVQETGKVQKCDLCGGEPACADACPTGAITYIDANWTGLDRMKQWAGKLDNQPAA"
     gene            4117..4236
                     /locus_tag="VGA59_14205"
     CDS             4117..4236
                     /locus_tag="VGA59_14205"
                     /inference="COORDINATES: protein motif:HMM:NF018050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720873.1"
                     /translation="MYFTRTAQEEVVLLTLYAKAKTDNITGAKLKEIRRALES"
     gene            4220..4534
                     /locus_tag="VGA59_14210"
     CDS             4220..4534
                     /locus_tag="VGA59_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9720874.1"
                     /translation="MPSKARPLTQEQLRTYEVKRDLAADLLESVRQMKAGKTQVVSSP
                     AVKARERTGLSQSQFAALLGVSVRTLQGWEQGRKQPSGAARTLLTIARTNPKALLAVA
                     GK"
     gene            complement(4814..5887)
                     /locus_tag="VGA59_14215"
     CDS             complement(4814..5887)
                     /locus_tag="VGA59_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX9720875.1"
                     /translation="MENLDVVVLRTLRDWRAAGKRALLATVVRTWGSSPRPVGSIMAL
                     CEDGAVVGSVSGGCIEDDLIYRFTRAYAGNEPGHEFPTGPPGFVKYGITADEAHRFGL
                     PCGGTLELLLEYDPDAAGLDALVRSLEAGQLMRRTVSLRDGTARLSKAGAPEELVLTE
                     GELVNSFGPEYRMLLIGAGQLTEYLATMALFNGFAVTVCDPREEYRGAWSVAGATVVA
                     DMPDDVVTAFKPDSRTCVVALTHDPKLDDLALLEALKTEAFYVGAIGSRRNNEARHQR
                     MIEHFGETEESLARLRGPIGIYIGSKTPPEIAVSVMAEILAVKNGVTLPRDMDVAQAK
                     NDRAVSANEPGDLVCAPARDRVA"
BASE COUNT         1012 a         2021 c         1899 g         1151 t
ORIGIN      
        1 atcccggagc ctgccccgga cctgatccgg ggtcgagccc gggatgacag ccacaacctg
       61 aaggcccggg atgacagcct cttagttcag cgacggcgtt catcccccgc gccctgccaa
      121 tggtcgcgcg cctgcgcgct ggcaaggtag gcctccatca ggcgcgtcgg gtcctgcttc
      181 aggtattcct tccacggccc cagccttacc tgtccttcga ccagcccgcg cagcacaccg
      241 acgtggtcgg tccagccgat gctgttgcag cccaccatca cgtcgccctt gaactgcagg
      301 ctgagatgcc ggccggccgc gcggtcggtc agttccacct ggtcgccgcc gggcacgccc
      361 tgccagtcgc cgaaactcgc cgagacgaga cccagcgtgt ccagaacgtt gatctgcgtc
      421 acgcccggca ataccgcctt ctgcccggcc atgttgagcc ctgcgacccg agcctggtct
      481 gcagcgttgg gctggatcgc actgaccatc atcttgccgc tgatcttgtc gaaggcttcg
      541 gcgcagtcac cggctgcgta gatgcctgga acgttggtct gcatgtgctc gtctgtcagt
      601 acacccagga ggcagcggat accggagttc tccaggaaac cgatggccgg cctgacgccc
      661 gtggcgctga tcaccaggtc ggccgtgagg cgcttgccgt tcgacagcct cacagacagt
      721 ggcgtggcgg tgtcgatggc ctcgacacgg gtgccggtga aaacctccac gcccttcatc
      781 tcgcaccact ccttgatcat gccgccggcg gtcgggccca tcatgcgcgg gaccatgcgg
      841 tcgcccatct cgaccaccga caggtggacg ccgcgcgcgg ccagcgcttc catgatgatg
      901 cagccgatga agccggcgcc catctgaagc acgcgtgcgc ccttgactgc cagccccatg
      961 atcttgcgcg catcctccag cgtccagcag ggatgcacgc ccggaagatc gatgcccgga
     1021 atgggcgggc gaatcggcgt ggagccggta gcgatcagca aggtgtcgaa ccgcagctgc
     1081 tggccgctgt ccagcgcgac gctgcgcgtg ccgatgtcga tgtgcgtggc gcgcccctgc
     1141 accagcttga tctgcaacgc gtcgaaatgg cccaggccct tgcgcagcca ggtgcccgct
     1201 tcggggatgt tccccatcag caggtaggga atcgccatgc gcgaataggg cggctctttc
     1261 tcgtcgccga cgatcgtgat cacatcctgc ggctggtgct tgcggatggt ctcggccgcg
     1321 atcacgcccg ccggaccggc accgaggatg acgtggtgca taacctagag ccctaagcgt
     1381 tggcgcgttt cgggcttgat cgtgccgtcc ggattccagc cccgcacctc gtagtatttc
     1441 gggagcatct tggccagctc gttcaccttg cccttggcgg ggccagtctg cgcgggttcg
     1501 gtgagcaggc gcgggggcag gttgtcgtcg gccgcggtga aacctgcgcg attgttgaag
     1561 tcgcgctcca tgttccagat gcgctcaccg atctttccca gctcttcagt gttgtagtct
     1621 tcgccgcatg ctgcggaaat ctgcggcgcc aagtcggcca ggccccatgc gaacgtcgtg
     1681 aagatgcaca gccccgacga gtcgaacgcg gccgtcgcat cctggaacgc tttcaccagt
     1741 tccggcttgc cttcgctcac cagtggatcg gtcttgaccg gaatacccag cacttcggag
     1801 gccacggtgt agccgcgcaa atggcaggcg ccacggttgc tcgtggcata ggccaggccc
     1861 atgccctgga taccgcggct gtcataggcg gggaactcct ggcctttcac gctcatggac
     1921 agctccggat gcccgtactt ggcggtcagc cgtttggagc cctggccgat ttccttgccg
     1981 aaaccctgcc ctgtcacggt ctgctcggcc aggaaggcca gcgcctgggc cgatccgaac
     2041 ggcgcttcga cgccgatctg ctccttggtc agcacgccca tctggtacag ctccatcacg
     2101 gcgccgatgg tggcgccgaa gctgatcggg tcgatgccgt cctcgttgca cagcaggttg
     2161 gcgtactgca gcgcctccag gtcgttgacg ccattggcgg cgcccagcgc ccacgccgct
     2221 tcgtattcca ggccgccgtt cgcgccccag tactgcggct tgttcgccac cgtgaagtgc
     2281 ccctcgtcga tcttgctgat gcgcccgcac gcaatcgtgc agccgaaaca ggcctggttg
     2341 gtcaccaggt gcttcttgcc gtcggtcttg cgcaccgcga ccatcgcttc ggcggaaatg
     2401 tccttggcgc cttcgaattg cacatccttg tggttgcgcg tcggcagagc ccccatttcg
     2461 ttgatcacgt tcatcagcac ctgggtgccg aaagccggca ggccctggcc cgtgacggcg
     2521 ttgtcggcga ggatcttctt cttttccttc accgccttca tgaacgcggc cgggtccttg
     2581 atgttgccaa cgcccttggt gccgcgcacc gcgatcgcct tcaggttctt gctgcccgcc
     2641 acggccccga cgccggagcg gcccgccgca cggtgcaggt cgttgacgat ggaagcgtag
     2701 agcacctggt tctcgccggc gcggccgatg ctggacacgc gcaccaaagg atcctgaagc
     2761 gtcttcttga gcagttcctc ggtctgccaa atggtcttgc cccacaggcc cgaagcgtcg
     2821 cgaagttcgg cgacatcatc gttgatgtac aggtacaccg gcgtgggcga cttgccctcg
     2881 acgatgacca tgtcccagcc ggccatcttc agttcggcgc cgaagtagcc gcccgaattg
     2941 gagcatgcga tcgcgccggt gagcggcccc ttggtgacta cggtgtagcg gccaccggtc
     3001 gatgccatcg tgcccgtgag cggaccggtg gaccagatca gcttgttggc gggcgagagc
     3061 ggatcgacct tggggtccat ctcttcgacc aggtatttgg atgccaggcc gcgcgagccg
     3121 atgtaggcct gagcccaggc catgttgagg ggctccggtg tcaccgtgcc cttggtgagg
     3181 tcgacgcgaa gaatttttcc agcccaagac atgattgttt ctccttcagg ctgcgggttg
     3241 attgtccaac ttcccggccc actgcttcat gcggtccagg ccggtccagt tggcgtcgat
     3301 gtaggtgatg gcaccggtcg ggcaggcgtc ggcacaggcc ggttcgccac cgcacaggtc
     3361 gcatttctgg accttgccgg tttcctgcac gtagttcacc gtgccgaacg ggcaggcgat
     3421 ggtgcagacc ttgcagccca cgcaggtgct gtcgttcacc accttggcgc ccgtgagccg
     3481 atcgatcgtg attgcctcca ccggacaggc atgcaggcac caagcctcgt cgcactgggt
     3541 gcaggtgtag ggcactttct tgccggtgtg atggaagtcg aacaccttga tgcgcgactt
     3601 ggccgtcgcg aaggttccat agttttcgaa cgagcaggcc atctcgcatt gcaggcagcc
     3661 cgtgcacttg tccgcgttga tgtgcagcac tttttgcatg tgcgtctctc ctgggaattt
     3721 cagcggctgc gccgcttggc ttatgaacgt gatcgcataa gcattgttaa tagcaaggtt
     3781 tctgatcagc ctagcaaaaa ccctaggcac gacgatttac acggtcaaat tctcaagatg
     3841 agacaggcaa gctcgacagc agcgaaatgc gcctcgcgga cgccccactg gataactatg
     3901 cgccttaggc gtatagtggc gtcaatgctc acggtggtcg aaactctctt gttccagaag
     3961 caatggccgc attactggac cgaggaagag ctgggtgagt tcgcgacgta catcgcggaa
     4021 tttccagacg ccggagacgt ggtgcctgag tccggcggga tctgaaaggt ccgctggcgt
     4081 cgggcaggtt ctggcaggtc cggtggagtt cgtgtcatct acttcacgcg cacggctcag
     4141 gaagaggttg ttcttctcac gctgtacgcg aaggcgaaga cggacaacat aacgggcgca
     4201 aagctaaagg agattcgccg tgccctcgaa agctagaccc ctcacccagg agcaactgcg
     4261 cacgtatgag gtcaagcgtg acctggccgc cgacctgctt gagtctgtgc gccaaatgaa
     4321 agcgggaaag acccaggttg tttcatcccc tgcagtgaaa gcgcgggaac gcacaggcct
     4381 gtcccagtca cagttcgcgg cgttgcttgg cgtgtccgtg cgaacccttc aaggatggga
     4441 gcagggccgc aagcaaccga gcggtgccgc gcgtaccttg ctcacgattg ctcgcaccaa
     4501 cccgaaggca ctgctagcgg tggctggcaa atgaccgagg ccggccaact gggttagctc
     4561 ccagtgagtg ccacgccgcg cttcggaaat gcgcgcagca gtttccggaa gtgcgcccat
     4621 accatcagac tcagtaccag cgcatgatcc ggcgcttgct tgatgcctat gtcgaagcgc
     4681 aagaggagcg ccctgtagct ggtcgctcaa gccgaacgac ggcccgcaag cgggccgccg
     4741 tttagcccac cgctgcccac actgctactt accgggccaa agtgaacttc cttcggccag
     4801 ccgacgcaaa atcctacgcc acgcgatccc tcgccggcgc gcacaccaga tcgcccggtt
     4861 cgttggcgct gacagcgcgg tcgttcttgg cctgggcgac gtccatatct cgcggcaggg
     4921 tgacgccgtt cttgacggca aggatttcgg ccatcacgct caccgcgatc tccggcggcg
     4981 tcttgctgcc gatgtagatg ccgatcggcc cccgcagccg cgccaggctt tcttcggtct
     5041 cgccgaaatg ctcgatcatg cgttgatggc gcgcttcgtt gttgcggcgg ctgccgatcg
     5101 cgccgacgta gaaagcctcg gtcttgagcg cctccagcag tgcgaggtcg tcgagcttgg
     5161 gatcgtgggt gagcgccacc acgcaggtgc gactgtcggg cttgaacgcc gtcaccacat
     5221 cgtccggcat atccgccacc acggtggcgc ccgcgacgct ccacgcgccc cggtattcct
     5281 cacgcggatc gcacaccgtc acggcaaacc cgttgaacag agccatcgtg gccaggtatt
     5341 cggtcagctg gcccgcgccg atcagcagca tccggtattc agggccgaaa ctgttcacga
     5401 gttcgccttc cgtgagcacc agttcttccg gtgcgcccgc cttgctcagc cgggcggtgc
     5461 cgtcccgcag gctgaccgtg cggcgcatca gctggccggc ttcgagtgag cgcaccagcg
     5521 catccaggcc ggcggcgtcg gggtcgtatt cgaggagcag ttccagcgtg ccgccgcagg
     5581 gcaaaccgaa ccgatgcgcc tcgtcggcgg tgatgccgta cttcacgaaa cccggcggcc
     5641 ccgtcgggaa ctcgtgaccc ggttcgttgc ccgcgtaggc ccgggtgaag cggtagatca
     5701 ggtcgtcctc gatacacccg cccgaaaccg agccgaccac cgcgccgtcc tcgcacagcg
     5761 ccatgatcga gccgaccggc cgcggcgacg aaccccaggt gcgcaccact gttgccagca
     5821 aagcgcgctt gcccgcggcg cgccagtcgc gcagcgtgcg cagcacgact acatcgaggt
     5881 tttccatgtg agaactcctt tcgtgccccg cgcttcgcaa cggcgctcaa cgcaacagcc
     5941 acagggccgc gagcagcagc acggctatgg cgacccaggc ccatacaggg attttcgcgc
     6001 gagccggctt gccggcgccg gcgtcagccc ccgcctgggt cgcggccgcc gcggcatagg
     6061 cctcggggtg ccggcgctgc att
//
LOCUS       DASWIY010000406        11936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2508865, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000406 DASWIY010000000
VERSION     DASWIY010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11936
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2508865"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VGA59_14220"
     CDS             complement(<1..288)
                     /locus_tag="VGA59_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720876.1"
                     /translation="MSFTMRYLSYFVTLLVAVLFTACGGGGGSPGITPGSQSFFTTAP
                     VDLTLALGSAQNFTVAGGRAPYSAVSNNSAVAVSAITDSTLTLGAVSPGVAL"
     gene            complement(317..2380)
                     /locus_tag="VGA59_14225"
     CDS             complement(317..2380)
                     /locus_tag="VGA59_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX9720877.1"
                     /translation="MVGWPFVVRAQTVIESVSSSLQGGSEVVRIDFSQPLQAIPAGFA
                     IQSPARIALDVPGATNGLGRSTVEINQGNLRSVNVVQAADRTRLVLNLKAAAAYKAQL
                     QGKSLLVVLEPVGVAGPAATAAPAFAESRNTETQPIRDVDFRRGSDSSARVIVDLPSN
                     QVGVDIRQQGQNLVVEFLKSSLPEGLRRRLDVSDFGTPVQTVTTFQAGDRVRVVIEPR
                     GAWEHSAYQSDNQFVVEVRQVKIDPTKLTQGPGFSGEKLSLNFQNIEVRSLLQVIADF
                     TNFNIVTSDSVTGAVTLRLKDVPWDQALDIVLQAKGLGMRKNGNVIWIAPKDEIAAKE
                     KLDLESRVAIQSLEQLRTQSFQLNYTKAADIAAQLTAGTGAARILSTRGSVIAEPRTN
                     QLFVTDITSRLEAVQQLIAKLDIAVRQVLIEARIVEASDTFGKSLGVRLGGSDLRGVR
                     GGDPGYAISGTNRLALGGTYNAVSSTTGESVNTLDTINTTFVNLPAIGQGGFTPSSFA
                     ISLFSAAANRFLNLELSALEADGKGKLVSSPRIVTADQTKAMIEQGTEFPYQQATSSG
                     ATAVAFRRATLKLEVTPQITPEGNIILDLDINKDSRGETTAAGIAINTKHIKTQVLVE
                     NGGTVVIGGIFELTETENEAKVPVLGDLPGVGNLFKTKSRTANKQEMLVFITPKMIAD
                     RAAAR"
     gene            complement(2434..2982)
                     /locus_tag="VGA59_14230"
     CDS             complement(2434..2982)
                     /locus_tag="VGA59_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEX9720878.1"
                     /translation="MLKNAATAATAVSVALLLAACGSSGQEELQAWMDEQRAQTRPKI
                     EPIPEPKKFTPQAYTQEALTDPFSSQKLTQALNRESAQSTSNAALVAPELARRKEPLE
                     SFPLDAMQMVGSMAKEGRPVALVRVDNLLYQVRPGNYLGQNFGKIMKVGETEVVLREI
                     VQDAAGEWIERTATLQLQERSK"
     gene            complement(2982..3653)
                     /locus_tag="VGA59_14235"
     CDS             complement(2982..3653)
                     /locus_tag="VGA59_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX9720879.1"
                     /translation="MATKQIPDINAAAAQQALLGQFRGLNTRDPASWPVLPRVALCLA
                     LLIAIVVALWFAWLESSDQELEAEQKKELVLREDYKKKLAQAVNLDALKKQREQVQQY
                     VTQLEKQLPSKAEMDALLSDINQAGLGRSLSFDLFRPGQVSVKEYYAELPITLRVTGR
                     FHDVGSFAADIANLSRIVTLNNLAATPNKDGTLVMDATAKTYRYLDGEEVAAQRKAAP
                     GAQKK"
     gene            complement(3659..4339)
                     /locus_tag="VGA59_14240"
     CDS             complement(3659..4339)
                     /locus_tag="VGA59_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX9720880.1"
                     /translation="MILINLLPHREAARKRRRESFYASLLVSALAGFVVAGGIYVWYA
                     TRISEQQSKNTVLSTEIKRLEAQIKDIATLQAEIAALRARQQAVEDLQADRNMPVHLV
                     NELVRQLPDGVYLTSLKQDNQVVTLQGVAQSNERVSELLRNLGNNSQWLTKPDLVEIA
                     SGTVALNARDTKRRVSNFTLRVRLLRATDVQKAASAASAASATGAASAAGGAAPAPVG
                     AASAPAKT"
     gene            complement(4336..5415)
                     /locus_tag="VGA59_14245"
     CDS             complement(4336..5415)
                     /locus_tag="VGA59_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX9720881.1"
                     /translation="MSSLGSLFSRQPAPLLGLDISSSSVKLVELGRDAAGNLVLERCA
                     IEPLERGWINDGNVEKFDEVAAAMRRVVKKSGTRAKHVAMALPPSAVITKKIILPGGL
                     TEQDLEAQVEAEANQYIPFSLDEVSLDFCVVGPSSTSAGDVEVLIAASRREKVQDRQG
                     LAEAAGLKPIIIDVESYASRLAASRLIQSLPNQGVDTMVALFEVGAFTTSMQVIRNDE
                     VLYDRDQAFGGAQLTQMIVRQYGFSLEEAENKKRNGDLPEDYESGVLKPFVESMAQEV
                     ARAMQFFFTSTPHNRVDYVMLAGGSAALPGLTDVVTMQTSFPCHLANPFEGMQVGAAV
                     REKKMRREAPSYLTSCGLAMRRFLQ"
     gene            5746..8151
                     /locus_tag="VGA59_14250"
     CDS             5746..8151
                     /locus_tag="VGA59_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEX9720882.1"
                     /translation="MAQRGWQRALLRASLWSVGLVLAGLASIVIIAGVALAVAYPNLP
                     DISDLSDYRPKLPLRVYSADGALIGEFGEERRNLTPIAQIPKVMKDAVLAIEDSRFYS
                     HGGVDYRGLIRAALANLGRIKSQGASTITMQVARNVYLSSEKTFTRKLYEVLLTFKLE
                     HLLSKDQILEIYMNQIFLGNRAYGFASAADTYFGKPMKDLTIAEAAMLAGLPKSPSSN
                     NPISNPSRARGRQLYIIERMLENGFITAEEAEAAKKESLKIRTGLDNTRVHAEYIAET
                     VRQLVYAQYGNEAYTRGLNVFTTVNSVQQDAAYRALRKGIMDYERRQIYRGPEEFVDL
                     PADPRDAEDAIDDALAEHPDNGDVMSAVVLEANPKKIVAVRQNGEKLEITGEGLRPAQ
                     SGLSNKAPPKIKIRRGAVIRVAKTPKNSWEITQLPEVEGAFVALDPRDGAIHALVGGF
                     DFNKNKFNHVTQAWRQPGSAFKPFIYSASLEKGFTPSTVVTDGPLFFDAGVTGGQPWE
                     PKNYDGKFEGPMPLHTALAKSKNMVSIRILQAVGTQNAQDWITRFGFDPEKHPPYLTM
                     ALGAGSVTPMQMATAYSVFANGGYRINPWLITKITDQRGKALVESQPPLPNESVRAVD
                     ARNAFIMSRLLQEIARAGTAARAQRELKRVDLYGKTGTTNDSIDTWFAGYQPTLAAVV
                     WMGYDTPRSLGDRETGGGLSLPVWISFMESALKGVPVMEPSAPEGVVNVGGEWYYEEY
                     ARGGGVGSAGGGSYENGTGAPAGIPRAPEDKSLMNSDGLPPSRAPSAPSEERRSILDL
                     FRN"
     gene            complement(8148..8474)
                     /gene="cyaY"
                     /locus_tag="VGA59_14255"
     CDS             complement(8148..8474)
                     /gene="cyaY"
                     /locus_tag="VGA59_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEX9720883.1"
                     /translation="MTDPEFMDRAEALLRRVEAGCDRINDETDADIDNQRVGGMVTLT
                     FPNGSQIVVNLQKPLHEVWMAARAGGFHYKFDGARWIDTKGNGEFFANLTRYAAEQAG
                     RPLDFA"
     gene            8487..8723
                     /locus_tag="VGA59_14260"
     CDS             8487..8723
                     /locus_tag="VGA59_14260"
                     /inference="COORDINATES: protein motif:HMM:NF025012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEX9720884.1"
                     /translation="MSNVFQILVSAHAARLALAASVVGALAGCGQRGPLYLPTGEAAA
                     GRATLPQTLMPSAPPARPASAPPATGTASPIRTP"
     gene            8720..10000
                     /gene="lysA"
                     /locus_tag="VGA59_14265"
     CDS             8720..10000
                     /gene="lysA"
                     /locus_tag="VGA59_14265"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX9720885.1"
                     /translation="MTITTLPGQPHFDYRGRELFAEGVGLRALVHEYGTPLFVYSKQA
                     MLAALAAYRRGFAGRKARICYAMKANSSLGVLQVFAQAGCGFDIVSGGELERVLAAGG
                     AAEDVIFSGVGKTRAEMRRALEAGVGCFNVESEGELEVLSEVALACGKTAPVSIRVNP
                     DVDPKTHPYISTGLKGNKFGVAHARTLAAYRRAAGLPGLRVAGIDCHIGSQITDEAPY
                     LDAMDRVLDLVQSIESAGIAIHHIDFGGGLGITYNGDRPPAADALWSKLFARLDARGF
                     GDRQVMIEPGRSLVGNAGVCLTEVLYLKPGEQKNFCIVDAAMNDLPRPSMYQGYHMIV
                     PVTRDSGGAVRDYDVVGPVCESGDWIGRDRSLAVQPGDLLAVLSAGAYCMSMASNYNA
                     RGRAAEVLVDGDRTHLIRERESAADLMRSERLVP"
     gene            complement(10174..10848)
                     /locus_tag="VGA59_14270"
     CDS             complement(10174..10848)
                     /locus_tag="VGA59_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEX9720886.1"
                     /translation="MAANGTWRKVVARALVHPAAKPLVFLLALMPFAWLLYGAITDNL
                     GANPAEYLSRSTGDWTLRFLCITLAVTPLRVITGTPALARFRRMTGLFVYFYVVLHFL
                     SYSWFDMGFDPADIAKDIAKRPFILVGFSAFLLLTPLAATSFNRAVKALGAARWQALH
                     KVVYAIASLAILHFFWMRAGKNDFAEVALYAAIIATLLGWRVAQYLKKKRLPARLAPS
                     SRRGLG"
     gene            complement(10848..11843)
                     /gene="msrP"
                     /locus_tag="VGA59_14275"
     CDS             complement(10848..11843)
                     /gene="msrP"
                     /locus_tag="VGA59_14275"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX9720887.1"
                     /translation="MLIQTRRDGFVHPTSSEITPRAVYQGRRDILKLLASGAAGTALA
                     SWASREALAQVQRPGKLAPLPGARSNAAGAATMEKITEYKDATSYNNFYEFGTDKADP
                     VKNAHTLKTSPWAVEVDGLVKKPAKYPLEDLLKLSAQEERIYRLRCVEGWSMVIPWVG
                     YSLSKLIEKVEPLGNAKFVEFISQADPKTMPFVGSRVLEWPYVEGLRLDEAMHPLTLL
                     AFGMYGEVLPNQNGAPVRLVVPWKYGFKSGKSLVRIRFTDREPKTAWNKAAANEYGFY
                     SNVNPEVDHPRWSQATERRIGEDGLFAKKRKTLMFNGYGSQVGQLYAGMDLKKNF"
BASE COUNT         2050 a         3872 c         3822 g         2192 t
ORIGIN      
        1 caaggcgacg cccggactta ccgcgcccag cgtcaaggtc gagtcggtga tcgcggagac
       61 cgcaaccgca ctgttattgc ttactgccga gtagggagct cttcctcccg ccaccgtgaa
      121 gttctgggcg ctgcccagtg cgagcgtcag atcgaccggc gcagtcgtga agaaggactg
      181 gctgcccggg gtgattccgg gagaaccccc tccgccccca caagcagtga aaaggacagc
      241 aactagcaat gtcacgaaat aggacagata acgcatggtg aagctcatgg ttagtaatgg
      301 ttattgaatc attgaatcaa cgtgccgcgg cacggtcggc gatcatcttg ggcgtgatga
      361 acaccagcat ttcctgcttg ttggcggtgc gcgacttggt cttgaacaga ttgccgacgc
      421 caggcagatc gcccaacacg ggaacctttg cctcgttctc ggtttccgtc agttcgaaaa
      481 tgccgccgat caccaccgtc ccgccgtttt cgaccagcac ctgggtcttg atgtgcttgg
      541 tgttgatcgc gatgcccgcg gccgtggttt cgccgcggct gtccttgttg atgtccaggt
      601 ccaggatgat gttgccctcc ggcgtgatct ggggtgtcac ctccagcttc agcgtggccc
      661 tgcggaatgc cacggccgtg gcgccgctcg aggtcgcctg ctgatacggg aactcggtgc
      721 cttgctcgat catcgccttg gtctggtcgg cggtgacgat gcggggactc gaaacgagct
      781 tgcccttgcc gtccgcctcc agcgccgaca gttcgaggtt gaggaagcga ttcgccgcgg
      841 cgctgaacag agaaatcgcg aagctcgacg gcgtgaagcc gccctgcccg atcgcgggca
      901 ggttcacgaa cgtggtgttg atcgtgtcca gtgtgttcac gctttcgccg gtggtcgatg
      961 acacggcgtt gtacgtgccc cctaacgcca ggcgattggt gccggaaatc gcgtagcccg
     1021 gatcgcctcc gcgtaccccg cgcaggtcgc tgccgcccag ccgcacaccc agcgacttgc
     1081 cgaaggtgtc ggaggcttcg acgatgcgcg cctcgatcag gacctggcgc accgcaatat
     1141 cgagcttggc aatgagctgc tgcaccgcct cgagccggga cgtgatgtcg gtgacgaaca
     1201 gctgattggt acgcggctcc gcgatcacgc tgccgcgcgt gctgaggatg cgggcggcgc
     1261 cggtgcccgc cgtgagctgg gccgcgatgt cggccgcctt ggtgtagttc agctggaagg
     1321 actgcgtgcg caactgctcc aggctctgga tggcgacacg cgattccagg tccagctttt
     1381 ccttggcggc gatctcgtct ttcggcgcga tccagatgac gttgccgttc ttgcgcatgc
     1441 ccagtccctt ggcctgcagg acgatgtcga gcgcctggtc ccacggcacg tccttcaggc
     1501 gcagggtgac ggcgccggtg acggagtccg aagtgacgat gttgaaattg gtgaaatcgg
     1561 cgatcacctg cagcagcgaa cgcacttcga tattctggaa attgagcgac agcttttcgc
     1621 cgctgaaacc ggggccctgg gtgagcttgg tgggatcgat cttcacttgc cgcacttcca
     1681 ccacgaactg gttgtcgctt tggtaggcgc tgtgttccca tgcgcccctg ggctcgatca
     1741 cgacgcggac cctgtcgccc gcctggaacg tcgtcaccgt ttgcaccggc gtgccgaagt
     1801 cggacacgtc gagccggcgg cgcaagcctt cgggaagcga ggacttcagg aattcgacca
     1861 ccaggttctg gccctgctgg cggatgtcga ctcccacctg gttgctcggc aggtcgacga
     1921 tcaccctggc cgagctgtcg ctgccgcgcc ggaagtccac gtccctgatg ggttgggttt
     1981 cggtattgcg gctctcggcg aaggcgggtg cggccgtggc cgcgggcccg gccaccccca
     2041 ccggttcgag taccacgagg agcgacttgc cctggagctg ggctttgtag gccgcggcgg
     2101 ccttcaggtt caggaccagg cgggtccgat cagcggcctg caccacgttc acggaccgaa
     2161 gattgccctg gttgatctcg accgttgagc ggcccaggcc attggttgca ccgggaacat
     2221 ccagcgcaat gcgcgccggc gactggatcg caaagccggc cggaatggcc tggagcggct
     2281 gcgaaaaatc gatgcgcacc acttcgctgc cgccctggag ggagctgctg accgattcga
     2341 tgacggtttg ggcccgcacc acgaacggcc agccaaccac gagaaagagg catgcgaagg
     2401 cccaggcctt gcgccaggtg atccggttct tgttcacttt gacctctctt gcagttgcaa
     2461 cgtggctgta cgttcgatcc actcgccggc ggcgtcctgg acgatttctc gcaggaccac
     2521 ctcggtttcc cctaccttca tgatcttccc gaagttctgt cccaggtaat tgccgggccg
     2581 gacctggtag agcaggttgt cgacacgcac cagcgcgacc ggccgcccct ctttggccat
     2641 gctccccacc atctgcatgg cgtccagcgg aaaactctcc agcggctcct tgcgccgggc
     2701 gagttccggt gccaccagtg ccgcgttcga ggtcgactgc gccgactcgc ggttgagcgc
     2761 ctgggtcagc ttctggctgc tgaaagggtc cgtcaatgct tcctgcgtgt aggcctgggg
     2821 cgtgaatttc ttcggctccg gaatcggctc tatcttggga cgagtctggg cccgctgctc
     2881 atccatccac gcctgcagct cttcctggcc ggaggaaccg caggcggcga gcagcaaagc
     2941 gacggaaacg gccgtagctg ccgtagctgc attcttaagc atcatttctt ctgtgctccc
     3001 ggcgccgcct tgcgctgagc cgcgacttcc tcaccgtcga ggtagcggta cgtcttggcg
     3061 gtggcatcca tcaccagcgt accgtccttg ttgggcgtgg cggcgaggtt gttgagcgtg
     3121 acgatgcgcg acaggttggc gatgtcggcc gcgaacgacc cgacgtcgtg gaagcgtccg
     3181 gtcacgcgca aggtgatcgg gagctccgcg tagtattcct tgacactgac ctggcccggc
     3241 cggaacaggt cgaagctcag gctgcggccc agcccggcct gattgatgtc cgacagcagg
     3301 gcgtccatct cggctttgct aggcagctgc ttctccagct gcgtcacgta ctgctgcacc
     3361 tgttcgcgtt gcttcttgag cgcatcgagg ttgacggcct gcgccagctt tttcttgtag
     3421 tcctcgcgca aaaccagttc tttcttctgc tccgcctcca gctcctggtc ggaactctcg
     3481 agccacgcga accacagtgc gaccacgatt gcgatcagca gtgccaagca cagcgcgacg
     3541 cgaggaagta ctggccaaga cgcggggtcg cgggtgttca ggccccggaa ttgtcccagc
     3601 agcgcctgct gggcggctgc cgcattgata tctggaatct gcttggtggc catggcgatc
     3661 aggtttttgc gggagctgaa gcggcgccaa ccggggccgg ggcggcgccg ccggccgcac
     3721 tggcggcgcc ggtcgcgctg gcggcgctgg cggcgctggc cgccttttgc acgtcggttg
     3781 cgcgcagcag acggacccgc agcgtgaagt tggacacgcg cctcttggta tcgcgcgcat
     3841 tgagcgcgac cgtgcctgag gcaatctcca ccaggtccgg cttggtcagc cattggctgt
     3901 tgttgcccag atttcgcagc agttcggata cgcgctcgtt ggactgcgcg acgccctgaa
     3961 gggtcacgac ctggttgtcc tgcttgaggc tcgtcagata gacgccgtcg ggcagctgcc
     4021 tgacgagttc gttcaccagg tggacgggca tgtttcggtc cgcctggagg tcctccaccg
     4081 cctgctggcg tgccctcagg gccgcgatct ccgcctggag cgtggcgatg tccttgatct
     4141 gtgcttccag ccgcttgatt tccgtggaca gaaccgtgtt cttgctttgc tgctccgaaa
     4201 tccgggtggc gtaccagacg tagatcccgc ccgcgacgac gaaacccgcc agtgccgaca
     4261 cgagcaatga cgcatagaag gactcacgcc tgcgtttgcg cgcggcctct ctgtggggaa
     4321 gcaggttgat caggatcact gcagaaacct ccgcatggcc aatccgcacg aggtgaggta
     4381 ggagggcgcc tcgcgccgca tcttcttctc gcgaaccgcg gcgcccacct gcatcccctc
     4441 gaagggattg gcaagatggc aggggaagga ggtctgcatc gtgaccacat cggtcaatcc
     4501 cggcagggcg gccgagccgc cggccagcat cacgtagtcg acccggttat gcggcgtgct
     4561 ggtgaagaag aactgcatcg cgcgggcgac ttcctgcgcc atgctctcca caaacggctt
     4621 gagcacgccc gattcgtaat cttccggcag gtccccgttt cgcttcttgt tttcggcctc
     4681 ttccagcgag aagccgtact ggcgcacgat catctgggtc agctgggcgc cgccgaatgc
     4741 ctggtcgcgg tcgtagagca cctcgtcgtt gcggatgacc tgcatgctgg tggtgaaggc
     4801 cccgacttcg aacagggcga ccatcgtgtc gacgccctgg ttgggcaggg actggatcag
     4861 ccggctggca gccagccggg aggcgtagga ctccacgtcg atgatgatcg gcttgagccc
     4921 ggcagcttcg gccaggccct ggcgatcctg caccttttcc ctgcgcgagg cggcgatcag
     4981 cacttcgacg tctccggccg acgtggaact cggcccgacc acgcagaaat cgaggctgac
     5041 ctcgtcgagc gagaacggga tgtactggtt ggcttcggct tcgacctggg cttccaggtc
     5101 ctgctcggtc aatccccccg gcaggatgat ttttttcgtg atgacggccg aagggggaag
     5161 cgccatcgcc acgtgcttgg cccgggttcc gcttttcttg acgacgcggc gcatggctgc
     5221 ggcgacctcg tcgaatttct cgacattgcc atcgttgatc cagccgcgtt cgagcggctc
     5281 gatggcgcag cgctccagca ccaggttccc cgcggcgtca cggcccagtt cgaccagctt
     5341 cacgctggaa gagctgatgt ccaagcccag cagtggcgcg ggctggcggc taaacaacga
     5401 ccccaaagag ctcaatttcc gtctcctgaa acgcccttgt ctccaggcgt aacaaactta
     5461 agatttcact tcaatgctaa ccgtaacgtc cttgtcacgt aaagactttt cccgttttta
     5521 atgatgagca gcgttgccaa aaaccgacgc cggaacctgc catactggta agcactcaca
     5581 tgtaagctcg gcgaacagat gacctggaat cagccccttc cgggacggcg aaatgagaac
     5641 gtcattttta taatgacctc ttgatgggac gcgatatgga tatttatgcc tgctgaaccc
     5701 tcctccgacg agcccggcgc gtcctcctcc tcgcgatcgc gcgggatggc gcagcgcggc
     5761 tggcagcgcg ccctgctgcg cgcaagcctc tggagcgtgg gcctggtact ggccggtctg
     5821 gcatcgatcg tcatcatcgc cggcgtggcg cttgcggtcg cctatcccaa tctgccggac
     5881 atctccgact tgtcggacta tcggcccaaa ttgccgctgc gcgtgtactc ggccgacggc
     5941 gccctgatcg gcgagtttgg cgaagaacgg cgcaatctga ccccgatcgc acaaatcccc
     6001 aaggtgatga aggacgcggt actggccatc gaggactcgc gtttctattc ccatggcggt
     6061 gtcgactacc ggggcctgat ccgcgctgcg ctggccaatc tgggccggat caagagccag
     6121 ggcgcctcga cgatcaccat gcaggtggcg cgcaatgtct acctctcgtc cgagaagacc
     6181 ttcacgcgaa agttgtatga agttctcctc acgttcaagc tcgaacacct gctgagcaag
     6241 gaccagatcc tcgagatcta catgaaccag atcttcctgg gcaaccgggc ctatggattt
     6301 gcctcggcag cggacactta cttcggcaag ccgatgaagg acttgaccat cgccgaggcg
     6361 gcgatgctcg cgggcctgcc caagtcgccc tcgagcaaca accctatcag caacccatcg
     6421 cgcgcccgcg ggcgccagct gtacatcatc gaacggatgc tggagaacgg tttcatcacc
     6481 gccgaggaag ccgaagcggc caagaaggag agcctgaaga ttcgcaccgg cctggacaac
     6541 actcgggtcc acgcggagta catagcggag acagtgcggc agctggtcta tgcccagtac
     6601 gggaacgaag cctacacgcg gggcctgaac gtcttcacga cagtcaattc cgtccagcag
     6661 gacgccgcgt acagggccct gcgcaagggc atcatggact acgagcggcg ccagatctat
     6721 cgcggtccgg aggaattcgt cgatttgccg gccgacccgc gggacgccga ggatgcgatc
     6781 gatgacgcat tggccgagca cccggacaac ggcgacgtga tgtcggccgt cgtgctggaa
     6841 gccaacccca agaagattgt cgcggttcgc cagaacggcg aaaaactcga aatcacgggc
     6901 gaaggactgc ggccggcgca atccgggctg tcgaacaagg cgccgcccaa gatcaagatc
     6961 cgtcgcggcg ccgtgattcg agtggccaaa acgccgaaga acagctggga gatcacgcag
     7021 ctgcccgagg tcgagggcgc cttcgtggcg ctcgatccgc gcgatggcgc catccacgcc
     7081 ctggtgggcg gcttcgattt caacaagaac aagttcaacc acgtgaccca ggcctggcgt
     7141 cagccgggct cggccttcaa gcccttcatc tactcggcct cgctcgaaaa gggcttcacg
     7201 ccttcgacgg tggtcactga cgggcccctg ttcttcgatg ccggcgtcac cggcgggcag
     7261 ccctgggagc cgaagaacta cgacggcaag ttcgaaggcc cgatgccgct gcacacagcg
     7321 ctggcgaagt ccaagaacat ggtgtctatc cggatcctgc aggcggtagg gacccagaac
     7381 gcccaggact ggatcacccg gttcggtttc gatcccgaaa agcatccccc ctacctgacc
     7441 atggcgctcg gcgcgggctc ggtgacgccg atgcagatgg ccaccgccta ttccgtcttc
     7501 gccaatggcg gctaccgcat caatccgtgg ttgatcacca agatcaccga ccagaggggc
     7561 aaggcgctgg tggaaagcca gccgccgctg cccaacgagt cggttcgcgc ggtggacgcg
     7621 cgcaacgctt tcatcatgag ccggctgctg caggagatcg ctcgcgcggg cacggccgcc
     7681 cgggcccagc gcgaactcaa acgggtcgac ctgtacggca agaccggcac gacgaacgat
     7741 tcgatcgata cctggttcgc cggctaccag cccacccttg ccgcggtggt ctggatgggc
     7801 tatgacacgc ccaggtcact cggcgaccgc gagacgggcg gcggcctgag ccttccggtg
     7861 tggatctcct tcatggagtc cgcgctcaag ggggtgccgg tgatggagcc gtcggcgccc
     7921 gagggcgtgg tcaatgtcgg cggcgagtgg tattacgagg agtacgcgcg gggcggcggt
     7981 gtcggcagcg cgggcggcgg ctcttacgag aatggcacag gtgctcctgc gggcattccg
     8041 cgcgcgcccg aagacaagag cctgatgaac tccgatggcc tgccgcccag ccgtgcgccg
     8101 tcggcaccgt cggaagaacg cagaagcatc ctggacttgt tcaggaacta ggcgaaatcc
     8161 agcggccgcc cggcctgctc ggcggcatag cgcgtgaggt tggcgaagaa ctccccgttg
     8221 cccttggtgt cgatccaccg cgcgccgtcg aacttgtagt gaaagcctcc ggcccgggcc
     8281 gccatccaga cttcatgcag gggtttttgc aggttcacga cgatctggct gccgtttggg
     8341 aaagtgagcg tgaccatccc gccgacccgc tggttgtcga tgtccgcatc ggtctcgtcg
     8401 ttgatgcggt cgcagccggc ttcgacgcgg cgcagcagcg cttcggctcg atccatgaat
     8461 tcggggtcgg tcattacaat ttcaccatgt cgaacgtttt ccaaattcta gtcagtgccc
     8521 atgccgcgcg gcttgccctt gccgcaagcg tggtcggggc gctggccggc tgcgggcaaa
     8581 gaggcccact gtaccttccg acgggcgaag cggccgccgg ccgggccact ttgccgcaaa
     8641 cgctgatgcc ctccgcgccg ccggcccggc ccgcgtcggc gccgcccgcc accggcaccg
     8701 cttcaccgat ccgcacccca tgaccatcac caccttgccg ggccagccgc acttcgatta
     8761 caggggtcgc gagctttttg cagagggcgt cggcctgcgg gccctggtgc acgaatacgg
     8821 cacacccctg ttcgtctatt ccaagcaggc gatgttggcg gccctggcag cctaccgtcg
     8881 cggtttcgcc ggccgcaagg cgcgaatctg ctatgcgatg aaggccaact cctcgctcgg
     8941 cgtgctccag gtgttcgccc aggcgggttg cggcttcgac atcgtctcgg gcggcgaact
     9001 cgagcgcgta ctggccgccg gcggggccgc cgaggacgtc attttttccg gtgtcggcaa
     9061 gacccgcgcc gagatgcgcc gtgcgctcga ggccggcgtc ggctgcttca acgtggagag
     9121 cgagggcgag ctcgaggtac tcagcgaagt ggccctcgcc tgcgggaaga ccgcccccgt
     9181 gagcatccgc gtcaacccgg acgtggaccc gaagacccac ccctacatct ccaccggcct
     9241 gaagggcaac aagttcggcg tcgcccacgc tcgcacgctg gcggcctacc ggcgcgccgc
     9301 gggcctgccg ggcctgcggg tggcgggcat cgattgccac atcggctccc agatcacgga
     9361 cgaggcgccg tacctggatg cgatggaccg cgtgcttgac ctggtgcagt cgatcgagtc
     9421 ggccggcatc gccattcatc acatcgactt cggcggcggg ctgggcatca cctacaacgg
     9481 cgaccggccg ccggcggcgg acgccttgtg gagcaagctg ttcgccaggc tcgatgcgcg
     9541 cggcttcggc gaccgccagg tcatgatcga gcccggccgc tccctggtcg gcaacgcggg
     9601 cgtgtgcctc acggaagtgc tgtacctcaa gcccggcgag cagaagaact tctgcatcgt
     9661 cgacgccgcg atgaacgacc tgcccaggcc gtcgatgtac cagggctacc acatgatcgt
     9721 gccggtgacg cgcgacagcg gcggcgcggt gcgcgattat gacgtggtcg ggccggtgtg
     9781 cgagagcggc gactggatcg gccgggaccg ctcgttggcc gtgcagcccg gcgatctgct
     9841 ggccgtgctg tccgccggtg cctattgcat gagcatggcc agcaactaca acgcccgcgg
     9901 ccgggccgcc gaggtgctgg tcgatggcga tcgcacccac ctcatccgcg agcgcgaatc
     9961 cgcggccgac ctgatgcgat ccgaacggct cgtgccctga aatgaaagcc cacccccgaa
    10021 gcgccttcgg cgcctccccc tcgagggggc gccaccagcg gcccggcaaa gccggttccg
    10081 cggtggcccg cgcctgcaga atcgactttc atccgtcgtg ggtgaagttc tccttcatgg
    10141 gaaactgagt tgccgcgcgc gacgccgcgg cgctcagccc agcccgcggc gcgaagaagg
    10201 agcgagcctt gccggaagcc gcttcttctt caggtactgc gccacacgcc agcccagcag
    10261 cgtggcgatg atggccgcgt aaagcgcgac ctcggcaaag tcgttcttgc cggcccgcat
    10321 ccagaagaag tgcaggatgg ccaatgaggc aatcgcatag acgaccttgt gcagggcctg
    10381 ccagcgagcc gcaccgagtg ccttcacggc gcgattgaaa gaggtcgccg ccagcggcgt
    10441 cagcagcagg aaagcgctga agcccaccag gatgaacggc cgcttggcga tgtccttggc
    10501 gatgtcggcc ggatcgaacc ccatgtcgaa ccagctgtag ctgaggaagt gcagcaccac
    10561 gtaaaagtag acgaacagcc cggtcatgcg gcgaaagcgc gcgagtgccg gcgttcccgt
    10621 gatgacgcgc aacggcgtca cggcaagcgt gatgcacagg aaacgcagtg tccagtcgcc
    10681 ggtggagcga gacaggtact ccgcgggatt ggcgcccagg ttgtcggtga tcgcgccgta
    10741 cagcagccag gcaaaaggca tgagcgcgag caggaacacc agcggcttgg ccgccgggtg
    10801 gacgagcgcc cgcgcaacca ccttgcgcca agtcccgttt gcggccatca gaagttcttc
    10861 ttcaggtcca tgccggcgta cagctgcccg acctggctcc cgtagccgtt gaacatcagg
    10921 gtcttcctct tctttgcaaa cagcccgtcc tcgccgatgc gccgttccgt cgcctggctc
    10981 cagcgcggat ggtcgacttc cgggttcacg ttcgaataga agccgtactc attggcggcc
    11041 gccttgttcc acgccgtctt cggctcgcgg tccgtgaagc ggatgcgcac caggctcttg
    11101 ccgctcttga agccgtactt ccatggcacc acgagccgca ccggcgcgcc gttctggttg
    11161 ggcagcacct cgccatacat gccgaaggcc agcagcgtca gcgggtgcat ggcttcgtcc
    11221 agacgcaagc cctccacata gggccattcg aggacacgcg agccgacgaa cggcatggtc
    11281 ttgggatcgg cctgcgatat gaactcgaca aacttggcgt tgcccagagg ctcgaccttc
    11341 tcgatcagct ttgacagcga gtagccaacc caagggatca ccatcgacca gccctccacg
    11401 cagcgcaaac ggtagatccg ctcttcctgc gcgctgagct tgagcaggtc ttccagcggg
    11461 tacttcgccg gtttcttgac cagcccatcg acctccacgg cccaggggct ggtcttgagg
    11521 gtgtgcgcgt tcttcaccgg atcggccttg tcggtgccga actcgtagaa gttgttgtag
    11581 ctcgtcgcgt ccttgtactc ggtgatcttt tccatggtcg ccgcgcccgc cgcgttggac
    11641 ctggcacccg gcagcggcgc gagtttgccc ggccgctgca cctgggccag ggcctcgcgc
    11701 gaagcccagg aggcgagcgc cgttcccgcc gcgcccgaag ccagcagctt gagaatgtcg
    11761 cgccggccct gatagaccgc gcgcggggtg atttcactgg aggtcggatg gacgaaacca
    11821 tcccggcgag tctggatcag catgcgtctt tcccgtcaat tacccgttgg tcggcagtcg
    11881 cgggccaatc ttacagagat aggacccggc cgctttcacc aagttccatg gggggg
//
LOCUS       DASWIY010000407         7008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2879809, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000407 DASWIY010000000
VERSION     DASWIY010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2879809"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VGA59_14280"
     CDS             complement(<1..297)
                     /locus_tag="VGA59_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720888.1"
                     /translation="MALRLKLPAFMARTRPRQRGEETMEGRLSLRERLVLLVVAAILP
                     LAALSVWFSVSEMESESQLAQSQLKFAASLIAAHQDNTVESAEHLLAAIAAMPMM"
     gene            complement(316..1248)
                     /locus_tag="VGA59_14285"
     CDS             complement(316..1248)
                     /locus_tag="VGA59_14285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX9720889.1"
                     /translation="MFRLLQLTFDFFETAAAAPAPHLEPKAPPERKRRSRDATERVAN
                     EPELLDQVLTPAAFRHPRANREALLGDSVVSYEFRRAKRRNIGFMVGPEGLTVSAPRW
                     VPLHEVDAAVKSKSSWIVKKLGDAHERHRRIESARIEWREGATFPFLGEQVIVVLDPR
                     HAFEEVGAVLHTDAETLPGVPRLTLHIGLPHTAGPAQIRDAVQAWLMRQAKRLFTERL
                     AHFAPRLGVQWRKLVLSNAGTRWGTAHADGLIRLNWRLVHFRLPVIDYVVAHELSHLR
                     VMDHSPRFWDTVRTVVPDYAQLRGQLKDEALPPW"
     gene            complement(1314..2048)
                     /locus_tag="VGA59_14290"
     CDS             complement(1314..2048)
                     /locus_tag="VGA59_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9720890.1"
                     /translation="MAFVRSTVHAIWMLLTVVPWAIAIVVASPWRSKISLYWMAAQWL
                     SWVIGGARVILGIEVRTLGMENLPVGQTSPAVLLVKHQSTLETFLMPTLMPHPLAYVF
                     KKELLQVPFFGWAMGRLDMVHIDRGLRAQAFSRVVEQGQRLLAQGIWVIIFPEGTRIP
                     RGQKGTYKHGGTRLAVATGAPVIPIAVTSAKVWPRKAFIKRPGVVDVSIGKPIPSQGR
                     DPDELMREVEDWIESEMHRLDPEAYG"
     gene            complement(2060..2629)
                     /gene="gmhB"
                     /locus_tag="VGA59_14295"
     CDS             complement(2060..2629)
                     /gene="gmhB"
                     /locus_tag="VGA59_14295"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEX9720891.1"
                     /translation="MKLVILDRDGTINADSDEFIKSPEEWTPLPGALEAIARLNHAGW
                     HTVIASNQSGLGRGLFDVASLNAVHAKMHKMLSAQGGRIDAVFYCPHGPDEGCLCRKP
                     SPGLFEQIGDRFGVDLKGAPVVGDGLRDLQAGVAAGCEPHLVLTGKGAAFRGRALPET
                     FPAGTVVHEDLAAFADWLIAHRPSVKIGA"
     gene            complement(2771..4876)
                     /gene="glyS"
                     /locus_tag="VGA59_14300"
     CDS             complement(2771..4876)
                     /gene="glyS"
                     /locus_tag="VGA59_14300"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX9720892.1"
                     /translation="MATHNLLVELFVEELPPKALKKLGEAFAGVLFEQLKAHGLVSAQ
                     SQLTSFASPRRLAAHVTAVAPRAEDRSVSQKLMPVSVGLDAGGAATPALLKKLQSLGA
                     DRSAVPGLKRAPDGKAEALFYDTTVKGATLAEGLQKALNESLAKLPIPKVMTYQLADG
                     WTDVKFVRPAHSLVALHGTQVVPVEVLGLKAGKTTHGHRFEAAVDPVRLEDADSYAAA
                     LERDGAVIASFEARRAEIVRQLADAAARMGGGAKPIEDEALLDEVTALVERPNVLVCE
                     FEKEFLEVPQECLILTMKANQKYFPLLDARGKLTNRFLVVSNIRPADASAVIGGNERV
                     VRPRLADAKFFFDQDRKKTLESRVPSLAKVVYHNKLGTQGERVERVRAIAKVIGAQLG
                     SDTLASHADRAAMLAKADLLTDMVGEFPELQGIMGRYYALNDGLGHEIADAIEDHYRP
                     RFAGDELPRNPTGLAVALADKLESLAGLFAVGQLPTGDKDPFALRRHALGIVRMLMEK
                     DLPLALDELVTESLGLFPQAKTGTAEQLTDFIYERLAGSLREQGYSPQEVDAVLALRP
                     QRLGDVVKRLAAVRAFAALPEAPALAAANKRIGNILKKADKADAHVSPVLLKEEAEKA
                     LYAMTREIAPRASSQFEAGDYTGSLQTLAALRGPVDAFFDGVMVNAEEADLRLNRLGL
                     LAILHQAMNRVADLSRLAT"
     gene            complement(4880..5785)
                     /gene="glyQ"
                     /locus_tag="VGA59_14305"
     CDS             complement(4880..5785)
                     /gene="glyQ"
                     /locus_tag="VGA59_14305"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX9720893.1"
                     /translation="MLTFQQIILKLQSYWDQQGCALLQPYDMEVGAGTSHTATFLRAL
                     GPEPWKAGYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPAPADILELYLGSLEALGF
                     DLKKNDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCKPITGEITY
                     GLERLAMYLQEVNNVYNLKWTEDLSYGDVYLQNEKEQSAYNFEHSDIDFLFAAFTAHE
                     KQARYLMEQQLALPAYEQVLKAAHSFNLLDARGAISVTERAAYIGRIRNLARSVAQSY
                     YDSRERLGFPMAPREWVAQMQKKAA"
     gene            complement(5822..>7008)
                     /gene="lnt"
                     /locus_tag="VGA59_14310"
     CDS             complement(5822..>7008)
                     /gene="lnt"
                     /locus_tag="VGA59_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX9720894.1"
                     /translation="RALAFAALWLLAELLRVTWFTGFPWGAGGYAHVDGPLSFLAPQV
                     GVHGIGFVAALLAFALSLLARRGTLRSWRYWAAIGGVAGLLAACNMVERPRQGGAMNE
                     PPLSVALLQGNIAQDEKFQGGTGVPLALDWYGRQLKESTASLVVAPETAIPMLPQQLP
                     QGYDDALVARFAQGRQAALIGIPLGSTKEGYTNSVVGLKPGAAQYRYDKHHLVPFGEF
                     IPPLFRWFTEMMNIPLGDFNRGPLGQAPFEWRGQRLAPNICYEDLFGEELAAQFADPA
                     RAPTILVNVSNIAWFGNTVAIDQHLHISRMRALEFERPMIRATNTGATVIIDHRGRVA
                     GSLPRHTRGVLTGEVEGRTGITRYAAWVSHLGLWPLWLLALAVVGVAAWRQRAGKSAA
                     GP"
BASE COUNT         1109 a         2421 c         2291 g         1187 t
ORIGIN      
        1 catcatcggc atcgccgcga tggcggcgag cagatgctcg gccgactcca ccgtgttgtc
       61 ctggtgggcc gcgatcagcg aggcagcgaa cttcagctgc gactgcgcca gctgggactc
      121 ggactccatc tcgctgacgg aaaaccagac cgacagcgcc gccagcggca agatcgcagc
      181 caccacgagc agcaccagcc gctcacgcag actcagcctg ccctccatcg tctcttcccc
      241 cctttggcgc ggccgggtcc tcgccatgaa cgcaggcagt ttcaggcgca gggccatgtc
      301 agcgagcgcc tgccgtcacc acggcggcag cgcttcgtcc ttgagctgtc cgcgcagttg
      361 cgcgtaatcg gggaccaccg tgcgcacggt gtcccagaac cgcgggctgt ggtccatcac
      421 gcgcaggtgg ctcagttcgt gcgccaccac ataatcgatc accggcagcc ggaagtggac
      481 cagccgccag ttcagccgga tcaagccgtc cgcatgggcg gttccccagc gggtgccggc
      541 gttgctgagg accagcttgc gccactgcac gccgagccgc ggcgcgaaat gcgcgagccg
      601 ttcggtgaac aggcgcttgg cctgccgcat cagccaggcc tggaccgcgt cgcgtatctg
      661 cgcggggccg gcggtgtgcg gcaggccgat gtgcagcgtg agccgcggca cgcccggcaa
      721 agtctcggcg tccgtatgga gcaccgcgcc cacctcctcg aaggcatggc gcgggtccag
      781 cacgacgatc acctgctcgc cgagaaatgg aaacgtcgcc ccttcccgcc attcgatgcg
      841 ggccgattcg atgcgccggt ggcgctcgtg cgcgtcgccg agcttcttga cgatccagct
      901 ggacttgctc ttcacggcgg catcgacttc gtgcagcggc acccacctgg gtgcgctcac
      961 cgtgagtccc tcgggtccga ccatgaagcc gatgttgcgc cgcttggcgc ggcgaaattc
     1021 gtaactcacg acggagtcgc ccagcagcgc ttcgcggttg gcgcgcgggt ggcgaaacgc
     1081 ggcgggcgtg agcacctggt ccagcagttc gggctcgttt gctactcttt ctgtagcatc
     1141 cctgcttctg cgcttgcgct ctggcggagc ctttggctcc aggtgggggg ctggcgcggc
     1201 ggcggccgtt tcaaaaaaat cgaaggtgag ctgcagcagc cggaacatgc cgccaagttt
     1261 agccgagccg tttgcgaatc actgtggttt tcgcacacgg caaaagggct tcgctaaccg
     1321 taagcctcgg ggtcgagccg gtgcatctcc gattcgatcc agtcctccac ctcgcgcatc
     1381 aattcgtccg ggtcacggcc ctggctcggg atcggctttc caatcgacac gtcgaccacg
     1441 ccgggccgct tgatgaaggc cttgcgcggc cagaccttgg ccgaagtcac cgcgatcggg
     1501 atcaccggcg cgccggtcgc cacggccagg cgtgtgccgc cgtgcttgta tgttcccttc
     1561 tgcccgcgcg ggatgcgcgt gccctcggga aagatgatga cccagatgcc ctgggccagc
     1621 agccgctgcc cctgttccac cacccggctg aaggcctgcg cgcgcagccc gcggtcgatg
     1681 tggaccatgt ccagccggcc catggcccag ccgaagaagg gcacttgcag cagttccttc
     1741 ttgaacacgt aggccagcgg atgcggcatc agcgtgggca tgaggaaggt ttccagcgtc
     1801 gactggtgct tgaccagcag caccgccgga ctggtctggc cgaccggcag gttctccatg
     1861 cccagggtac gcacttcaat gcccaggatg acacgggcgc cgccgatcac ccagctcagc
     1921 cactgcgccg ccatccagta caaggagatc ttgctgcgcc agggcgaggc cacgacgatg
     1981 gcgatggccc atggcaccac cgtgagcagc atccagatgg cgtggaccgt cgagcggacg
     2041 aacgccatga gccggccgat caggcgccga tcttgacgct gggccggtgc gcgatcagcc
     2101 agtccgcgaa ggccgcgagg tcctcgtgca ccacggtgcc ggccggaaag gtttcgggca
     2161 gcgctcgccc gcggaaagcc gcgcccttgc ccgtcagcac caggtgcggc tcgcaaccgg
     2221 ctgcgacgcc ggcctgcagg tcgcgcagcc cgtcgcccac gaccggagcg cccttgaggt
     2281 cgaccccaaa gcggtcgccg atctgctcga acaggccggg agagggtttg cggcacaggc
     2341 agccttcgtc cgggccgtgc gggcagtaga acaccgcgtc gatgcgtccg ccctgcgccg
     2401 aaagcatctt gtgcatcttg gcatgcaccg cgttgagcga ggcgacgtcg aacagcccgc
     2461 gtcccagccc cgactggttg gaggcgatca ccgtgtgcca gccggcgtgg ttgagccgcg
     2521 cgatcgcctc gagcgcgccc ggcagcggcg tccactcttc cggcgacttg atgaattcgt
     2581 cgctgtcggc gttgatggtt ccgtcgcgat cgaggatgac gagcttcatg gctgggcctc
     2641 cgccgcccgg cgttgtatcc aggcggcatt gcgcagcctc gcaacggctc gccgatggaa
     2701 agcgagcgta aaccggtcaa cgtcttgttg attgggcatg gacaaatggt gctgcgccgg
     2761 cactgcggcg tcaagtcgcc agccgcgaaa gatcggcaac ccggttcatg gcctggtgca
     2821 ggatggccag caagcccagg cgattgaggc gcaggtccgc ctcttcggcg ttcaccatga
     2881 cgccgtcgaa gaaagcgtcg accggtccgc gcagggcggc cagggtctgc agcgagccgg
     2941 tgtagtcgcc cgcctcgaac tgcgaactgg cgcgcggcgc gatctcccgc gtcatcgcgt
     3001 acagcgcctt ctcggcctct tccttgagca gcaccgggct cacatgggcg tcggccttgt
     3061 cggctttctt gaggatgttg ccgatccgct tgttggccgc cgccagcgcc ggcgcttcgg
     3121 gcagggccgc gaacgcgcga acggcggcca gccgcttgac cacgtcgccc aggcgctgcg
     3181 ggcgcagcgc cagcaccgcg tccacctcct gcgggctgta gccctgctcg cgcagcgagc
     3241 cggccaggcg ctcgtagatg aaatcggtca gttgctccgc ggtccctgtc ttggcttggg
     3301 ggaacagccc cagcgactcc gtgaccaact cgtccagcgc cagcggcagg tccttctcca
     3361 tcagcattcg cacgatgccc agcgcgtggc gccgcagcgc gaacgggtcc ttgtcgccgg
     3421 tgggcagctg gcctaccgcg aacagcccgg cgagcgactc cagcttgtcc gccagcgcca
     3481 ccgccagccc ggtcgggttg cgcggcaact cgtcgccggc aaagcgcggc cggtaatggt
     3541 cctcgatggc gtcggcaatc tcgtggccca gaccgtcgtt gagggcgtag taacggccca
     3601 tgatgccctg cagctcgggg aattcgccca ccatgtcggt gagcaggtcc gccttggcaa
     3661 gcatcgccgc ccggtcggca tgcgacgcca atgtgtcgct gccgagctgg gcgccgatca
     3721 ccttggcgat cgcgcgcacg cgctccaccc gctcgccttg cgtgcccagc ttgttgtggt
     3781 agaccacctt ggcgaggctt ggcacccgcg actccagcgt tttcttgcgg tcctggtcga
     3841 aaaagaactt ggcgtcggcc agccgcgggc gcaccacgcg ctcgttgccg ccgatcacgg
     3901 cgctggcgtc cgccggacgg atgttgctca cgaccaggaa cctgttggtc aatttgccgc
     3961 gcgcgtcgag cagcgggaag tacttctggt tcgccttcat cgtgaggatg aggcattcct
     4021 gcggcacctc caggaattcc ttctcgaatt cgcagaccag gacgttgggg cgctcgacca
     4081 gcgcggtgac ttcatcgagc agggcttcgt cctcgatcgg cttcgcgccg cctcccatcc
     4141 tggccgccgc gtcggcaagt tgccgcacga tctcggcgcg gcgcgcctcg aagctggcga
     4201 tcacggcgcc gtcccgttcc agggcagccg catagctgtc ggcgtcttcc agccgcacgg
     4261 gatcgacggc cgcctcgaag cggtggccgt gagtggtctt gccggccttc aggcccagca
     4321 cttcgaccgg tacgacctga gtgccatgca gcgcgaccag cgaatgggcc ggccgcacga
     4381 atttcacgtc ggtccagccg tcggccagct ggtacgtcat gaccttgggg atcggcagtt
     4441 tcgcgaggct ttcgttcagc gccttctgca ggccttcggc cagcgtcgcg cccttgacgg
     4501 tggtgtcgta gaacaatgcc tcggcttttc cgtccggtgc gcgcttgagg cccggcacgg
     4561 cggagcggtc ggcgcccagc gactgcagct ttttcaacag cgccggtgtc gcggcgccgc
     4621 ccgcatcgag ccccacgctg accggcatca gcttctgcga aacggacctg tcttcggcgc
     4681 gcggcgccac cgcggtgaca tgagccgcga gccggcgcgg cgaggcgaac gaggtcagtt
     4741 gcgactgcgc cgaaaccagc ccatgcgcct tcagctgctc gaacaacaca ccggcaaacg
     4801 cctcacccag cttcttcagc gccttgggcg gcagctcttc gacgaacagt tcgaccagaa
     4861 ggttatgcgt tgccatgtct tacgccgcct tcttttgcat ctgcgccacc cactcgcgcg
     4921 gcgccatcgg aaaaccgagg cgctcgcggc tgtcgtaata gctctgggcc acgctgcgcg
     4981 ccaggttgcg gatgcggccg atgtaggccg cccgctcggt cacgctgatc gcgccgcgcg
     5041 catccagcag gttgaagctg tgcgcggcct tgagcacctg ctcgtacgcc ggcaacgcga
     5101 gctgctgctc catcaggtac ctggcctgct tctcgtgagc cgtgaacgcc gcgaacagaa
     5161 agtcaatgtc gctgtgctcg aagttgtagg cggactgctc tttctcgttc tgcaggtaca
     5221 cgtcgccgta gctcaggtct tcggtccact tcaggttgta gacgttgttg acctcctgca
     5281 ggtacatggc gaggcgctcc agcccataag tgatctcgcc ggtgatcggt ttgcagtcga
     5341 tgccgccgac ctgctggaaa taggtgaact gcgtgacttc catgccgttg agccagactt
     5401 cccaacccag gccccacgcg cccagcgtcg ggttctccca gtcgtcttcg acaaagcgga
     5461 tgtcgttctt cttcaggtcg aaccccagcg cttcgagcga gcccaggtac agctccagga
     5521 tgtcggccgg cgccggcttt agcaccacct ggtactggta atagtgctgc agccggttcg
     5581 ggttctcgcc gtaacggcca tccttgggac ggcggctggg ctgcacatag cccgctttcc
     5641 agggctcggg gcccagcgcg cgcaggaaag tggcggtgtg cgaggtcccc gcgcccactt
     5701 ccatgtcgta gggctgcagc agggcgcagc cttgctggtc ccagtaggac tgcaatttca
     5761 ggatgatttg ctggaaggtc aacatggagc aatgagggcc gcgcgcggca aaccgttgat
     5821 tttacggccc tgctgcgctc ttgccggccc gctgccgcca cgcggcgacc ccgacgaccg
     5881 ccagcgccag cagccatagc ggccacagcc cgaggtgcga gacccacgcc gcatagcggg
     5941 taatgcccgt gcgcccctcg acctcgccgg tcagcacgcc gcgggtgtgg cgcggcagcg
     6001 agcccgcgac gcgtccgcgg tggtcgatga tcaccgtggc gcccgtgttc gtggcgcgga
     6061 tcatcgggcg ttcgaactcc agggcgcgca tccggctgat gtgcaggtgc tggtcgatgg
     6121 ccacggtgtt gccgaaccag gcgatgttgc tgacgttgac gaggatggtg ggcgccctgg
     6181 ccggatcggc gaactgcgcg gccagctctt cgccgaacag gtcctcgtag cagatattgg
     6241 gcgccaggcg ctgaccccgc cactcgaacg gcgcctgccc cagcggcccc cggttgaagt
     6301 cacccagcgg aatgttcatc atctcggtga accacctgaa cagcggaggg atgaattcgc
     6361 cgaacggcac caggtggtgc ttgtcgtaac ggtactgggc ggcgcctggc ttcaggccaa
     6421 cgacggaatt cgtatagccc tctttcgtgc tgcccagcgg aatgccgatc agcgctgcct
     6481 ggcgcccctg ggcaaagcgc gccaccagtg cgtcgtcata gccttgcggc aactgctgcg
     6541 gcaacatggg gatcgccgtc tcgggcgcaa ccaccaggct agcggtgctt tctttcaatt
     6601 gccgcccgta ccagtccagc gccagcggca cgccggtgcc gccctggaac ttctcgtcct
     6661 gcgcaatgtt gccctggagc agcgcgaccg aaagcggcgg ctcgttcatc gccccgccct
     6721 gcctgggacg ttccaccatg ttgcaggcgg ccagcagccc cgccacgccc ccgatcgcgg
     6781 cccaatagcg ccacgaccgc agcgtccccc ggcgcgccaa cagggacagc gcgaaagcca
     6841 gcagcgcggc cacgaagccg atgccgtgca cgcccacctg gggcgccagg aaagacagcg
     6901 gtccgtccac atgcgcatag ccgccggcgc cccagggaaa gccggtgaac caggtgacgc
     6961 gcagcaattc ggccagcagc cacaaggccg cgaaggccag agcgcgca
//
LOCUS       DASWIY010000408         7449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3583713, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000408 DASWIY010000000
VERSION     DASWIY010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3583713"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..821
                     /locus_tag="VGA59_14315"
     CDS             144..821
                     /locus_tag="VGA59_14315"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX9720895.1"
                     /translation="MKSVQPCGLRRCAWPIGFLVGILFMATASVQAGSVLETRGEVWV
                     ERAQQLVPMRAGTALQEGDKLITAPGAEVLVRLEDDARLLVRADSRLDLRQLLRRDEL
                     RKQIISIVKGGLRYVSSVSTVRQQVNFTSAQATIGIRGTDIEIAMGEGANAKPVTYLK
                     VNRGIAVLTGLDGTQVELTEGQIALALEEELSRAGVRSIPHPSAMRMEAAPAHLFQPG
                     SLDRLLR"
     gene            complement(839..1693)
                     /locus_tag="VGA59_14320"
     CDS             complement(839..1693)
                     /locus_tag="VGA59_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9720896.1"
                     /translation="MHPNLYALGAIALWATLASLGVSLRHVPPFLLTGLALVIGSMPA
                     WPLLRQWRVPPSTLALGVYGLFGFHFLLFIALRHAPPVEANLVNYLWPLLIVVMAPLI
                     LPGMRLRAPHLIAALVGFAGAAIAILGRGGAGGGWSWGYLPALGSAFIWASYSLLTRR
                     VRPFPTAAIGLFGLVSGVLSLLCHWALEPPAPLQAGDWALISAMGLGPLGAAFFLWDK
                     ALKLGDARHIGILSYLTPLASTLLLMLVSGSAFSWSITAAALLIIGAAVAGTRPVDER
                     STRPAAEA"
     gene            complement(1701..2690)
                     /gene="lipA"
                     /locus_tag="VGA59_14325"
     CDS             complement(1701..2690)
                     /gene="lipA"
                     /locus_tag="VGA59_14325"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX9720897.1"
                     /translation="MSSNEVVREAQPVETYQAGAKQKAAAKLSRIPVKVEQAEVLRKP
                     EWIRVKAGSPSTRFYEIKQILRESHLNTVCEEASCPNIGECFGKGTATFMIMGDKCTR
                     RCPFCDVGHGRPDPLDANEPHSLARTIARLKLKYVVITSVDRDDLRDGGAGHFVDCIR
                     ATRELSPGTTIEVLVPDFRGRDDRALEILKAAPPDVMNHNLETIPRLYREARPGSDYA
                     FSLNLLKKFKALHPHVPTKSGLMVGLGETDEEILQVMRDMREHHIEMLTIGQYLAPST
                     SHLPVRRYVHPDTFRMFEAKAREMGFSHAAVGAMVRSSYHADQQAHAAGVTSA"
     gene            complement(2702..3493)
                     /gene="lipB"
                     /locus_tag="VGA59_14330"
     CDS             complement(2702..3493)
                     /gene="lipB"
                     /locus_tag="VGA59_14330"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX9720898.1"
                     /translation="MEAVDRGRVEYVPTYQAMRAFTAARTPDTADELWLCEHAPVFTQ
                     GLAGRADHVLDAGAIPVIATDRGGQVTYHGPGQVVAYPLVDLRRSGYFVKEYVFRIEE
                     AVIRTLGYFGVTGHRVAGAPGIYVRLDDPFGHARLPSPQPSPASGRGSNTEASGRGSG
                     GEMTLPLRTGRTELPLPPAGEGGGEGRFQGLGKIAALGIKVSRNCTYHGVALNVAMDL
                     EPFARIDPCGYPGLKTVDLSTIGVSTTWNEAARVLGAKLSAHLAP"
     gene            complement(3494..3793)
                     /locus_tag="VGA59_14335"
     CDS             complement(3494..3793)
                     /locus_tag="VGA59_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HEX9720899.1"
                     /translation="MSKPAVPPSAPQDPKQSLIQYPSRFPIKVMGAKVDGFVHAVTTI
                     ARQFDPTFDAASIELRDSKAGNYLGVTITITATSREQLDELYRTLSTHPMVKVVL"
     gene            complement(3955..4839)
                     /locus_tag="VGA59_14340"
     CDS             complement(3955..4839)
                     /locus_tag="VGA59_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEX9720900.1"
                     /translation="MNPYQLPDLPCYLNGEFTTLREAKVSVMDRGFIFGDGVYEVVPV
                     YAGRPFRFAQHMARLARSLLELRITNPMDPAQWRELVDRLVAAYAKTLGKNPGETDQL
                     VYLQVTRGVAMRDHVMPPDITPTVFASSNRMSPPSAQDRAAGVACVSGEDFRWKKAHI
                     KSISLAGAVLARQMSADVGAAETVMFRDGWLSEAAACNVWVVSNGTVMGAPKSNLVLE
                     GIRYGLIEELCGQAGIPFQLRPISRDEVFAADELMLSSATKEVLPVTRLDQQTIGTGR
                     PGPIYEQLYAGYQRAKEK"
     gene            5004..5489
                     /locus_tag="VGA59_14345"
     CDS             5004..5489
                     /locus_tag="VGA59_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX9720901.1"
                     /translation="MKKIAPLPETTEEEPPFKPLTAEEARRLREEHPLVSPWWVVAGQ
                     IVVGVVVALAAWALTGRQNVGWSAGYGALVVVIPAAIFARGLTGRFSSLNAVTAAVGF
                     LVWEMVKIASSIALLAAAPRLVPELSWPALLVGLVLTIKVYWVALAYSPRKGRAANVK
                     L"
     gene            5501..6358
                     /gene="atpB"
                     /locus_tag="VGA59_14350"
     CDS             5501..6358
                     /gene="atpB"
                     /locus_tag="VGA59_14350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX9720902.1"
                     /translation="MAAEHGPTAGEYIIHHLKHLQNHKATNVVDFSVFNFDSMFFSTA
                     LGILVCWVLWLAARKATSGVPGRFQAAVEILVEMVDTQAKGIVHNAQSRKFVAPLALT
                     VFIWIFMLNAMDLLPVDLLPWIWERIYAATGNDPHEAYLRVVPTADLSMTMGLSVSVL
                     LICLFYNIKIKGLGGWVHELFTAPFGAHPALWPFNFAMQMIEFIAKTVSHGMRLFGNM
                     YAGELIFLLIALMGGAWSLSATGIGLAIGHIIAGTAWAIFHILIITLQAFVFMMLALV
                     YLGQAHDAH"
     gene            6418..6684
                     /gene="atpE"
                     /locus_tag="VGA59_14355"
     CDS             6418..6684
                     /gene="atpE"
                     /locus_tag="VGA59_14355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX9720903.1"
                     /translation="MMEVISFVALATGLIIGLGAIGACIGIGIMGSKYLESAARQPEL
                     MGELQTKMFLLVGLIDASFIIGVGIALWFATANPFLAQIANLPK"
     gene            6742..7212
                     /locus_tag="VGA59_14360"
     CDS             6742..7212
                     /locus_tag="VGA59_14360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX9720904.1"
                     /translation="MSITGTLIIQMIVFLILVGFTMKFVWPPIAAALDERARKIADGL
                     AAADKAKAELAAADRRVEEELGKTRTETAALLADAERRGQQIVEEAKARALEEANKII
                     AAAKVEADQQSVKAREALREQVAALAVKGAEQILRKEVNASVHAELLERLKTEL"
     gene            7222..>7449
                     /locus_tag="VGA59_14365"
     CDS             7222..>7449
                     /locus_tag="VGA59_14365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX9720905.1"
                     /translation="MAELATIARPYAEALYKSARSDLNGASLWLDALAAVAGNAQLLQ
                     FANSPKVTDQQVFDVMADVAKEPLPENGKNFL"
BASE COUNT         1317 a         2352 c         2465 g         1315 t
ORIGIN      
        1 aagcaggaag tgctgcgagc cgatacggcc cggctggcgc cctgggcacg tgaggtgctc
       61 gacgccgagg acccggcaca agcgatggcg cgctaaacgt accacctcgt taccatggat
      121 actttcaaag aggcggggtc cgaatgaaat ctgtccagcc atgtggactg cgccgctgtg
      181 cctggccgat cgggtttctc gtcggaattc tgttcatggc gacggcgtcc gtgcaggccg
      241 gctccgtcct cgagacgcgc ggggaggtat gggtcgagcg cgcgcaacag ctcgtgccga
      301 tgcgcgcggg tacagcgctt caggaggggg acaaactcat caccgccccg ggcgccgagg
      361 tcctggtgcg cctcgaagac gacgcccgcc tgctcgttcg cgccgattcc aggctggacc
      421 tgcgccaact gctgcgcagg gacgaactgc gcaagcagat catcagcatc gtcaaaggtg
      481 ggcttcgcta tgtttcgagc gtgagtacgg tgaggcaaca ggtgaatttc acctcggcac
      541 aggcaaccat cggaatccgg ggaacggata tcgaaatcgc aatgggtgaa ggcgcaaacg
      601 cgaaaccggt cacctatctc aaggtcaatc gcggaatcgc ggtcttgacg gggctggatg
      661 ggactcaggt cgagctaacc gaaggccaga tcgcacttgc cctcgaagag gagctctcgc
      721 gggccggcgt gcgcagtatc ccccatccat cggcgatgag aatggaagct gccccggcac
      781 accttttcca gcctggctca ctggaccgcc ttctgcgcta gttcgccgtc gacttttttt
      841 atgcctcagc ggcaggtctg gtacttcgct cgtcaaccgg gcgcgtgccg gccacagccg
      901 caccgatgat caggagcgcg gcggccgtga tgctccagct gaacgcactg ccgctgacca
      961 gcatcagcag cagcgtcgaa gccaggggcg tcaggtagct caggatgccg atatggcgcg
     1021 catcgcccag cttgagcgct ttgtcccaca ggaaaaaagc cgcgcccagc gggcccaggc
     1081 ccattgcgga aatcagcgcc caatcgccgg cctgcagcgg cgcggggggt tcgagagccc
     1141 agtggcacag cagcgagaga acgcccgaga ccagaccaaa caggccgatg gccgcggtcg
     1201 gaaaggggcg cacccggcgc gtgagcagcg agtagctggc ccagatgaag gccgaaccca
     1261 gggccggcag gtaaccccac gaccagccgc cgcccgcgcc tccacgcccc aggatcgcga
     1321 tggcggcgcc tgcgaagcca accagggccg cgatgaggtg cggcgcgcgc aaacgcattc
     1381 ccggaagaat cagcggggcc atgaccacga tcagcagcgg ccagaggtag ttgaccaggt
     1441 tggcctcgac gggcggggcg tggcgcaagg cgatgaacag caggaaatgg aagccgaaca
     1501 ggccatagac gcccagcgcc agggttgacg gcggaaccct ccattggcgc agcagcggcc
     1561 aggccggcat gctgccgatc accaaggcca gcccggtcag caggaaaggc ggcacatgcc
     1621 gcagcgagac gccgagcgat gccaacgtgg cccatagcgc gatcgcgcca agggcgtaaa
     1681 gattcggatg cagggccctg tcaggcggag gtgacaccgg cggcgtgggc ctgctggtcg
     1741 gcgtggtaac tggagcgcac catcgccccc acggccgcat ggctgaagcc catctcgcgg
     1801 gccttcgcct cgaacatcct gaaggtgtcg gggtgcacgt agcggcgcac cggcaggtga
     1861 gacgtcgacg gcgccaggta ctgcccgatg gtgagcatct cgatgtggtg ctcgcgcatg
     1921 tcgcgcatca cctgaaggat ttcctcgtcg gtttcgccca ggcccaccat caggccgctc
     1981 ttggtcggga catggggatg cagcgccttg aacttcttga gcaggttcag gctgaacgcg
     2041 taatcggagc cgggccgcgc ttcccggtac aggcggggaa tcgtttcgag gttgtggttc
     2101 atcacatccg gcggcgcggc cttgaggatt tccagcgccc ggtcgtcgcg gccgcggaag
     2161 tcgggcacca gcacctcgat ggtcgtgccg ggcgacagct cgcgcgttgc acggatgcag
     2221 tccacgaaat gccccgcgcc gccgtcgcgc aggtcgtcac ggtccacgga agtgatgacg
     2281 acgtatttca gcttcagccg ggcgatggtc cgtgccaggc tgtgcggctc gttggcgtcc
     2341 agcggatcgg gccgcccatg gcccacgtcg cagaacgggc agcggcgcgt gcatttgtcg
     2401 cccatgatca tgaaggtcgc ggtgcccttg ccgaagcatt cgccgatgtt ggggcacgac
     2461 gcttcctcgc acacggtgtt gagatggctc tcgcgcagga tctgcttgat ctcgtagaag
     2521 cgcgtgctgg gagacccggc cttgacgcgg atccactcgg gcttcctgag cacctcggct
     2581 tgctcgacct tgaccggaat gcgcgacagc ttggccgccg ccttctgctt ggcgccggcc
     2641 tgataggttt cgaccggctg cgcctcgcgc acgacttcat tggagctcat gggagttgtt
     2701 gtcagggcgc caagtgggcg ctgagcttgg cgcccagcac tcttgccgcc tcgttccagg
     2761 tggttgatac cccgattgta gaaagatcca ccgtcttcag cccggggtag ccgcagggat
     2821 cgatgcgcgc gaaaggttcg aggtccatcg caacgttgag cgccacgccg tggtaggtgc
     2881 agttccggct gaccttgatg cccagggcgg cgatcttgcc caggccctga aagcgaccct
     2941 cacccccgcc ctctcccgca ggcgggagag ggagttctgt tcgccctgtc cgaagcggca
     3001 gcgtcatttc gccaccgctc cctctcccgc ttgcctcggt attgctccct ctcccgctgg
     3061 cgggagaggg ttggggtgag ggtaagcggg catgcccgaa cggatcgtct agccgcacgt
     3121 agatgcccgg cgcgcccgcc acccggtgac ccgtcacgcc gaaatagccg agtgtgcgga
     3181 tgacggcttc ctcgatgcgg aacacgtatt ccttgacgaa gtagccgctg cggcgcaggt
     3241 cgaccagcgg ataagccacg acctgccccg gcccgtggta ggtaacctgg ccgccgcgat
     3301 cggtggcgat cacgggaatc gcgccggcgt cgagcacatg atcggcccgg ccggccaggc
     3361 cctgcgtgaa caccggcgcg tgctcgcaca gccagagctc gtcggccgtg tccggagtgc
     3421 gggccgcggt gaatgcccgc atggcctggt aggtgggcac gtattccacg cgcccgcggt
     3481 cgaccgcttc catctaaagg acgaccttga ccatgggatg cgtggacagc gtgcggtaaa
     3541 gctcgtcgag ctgctcgcgg ctggtcgcgg tgatggtgat cgtgacgccg aggtaattgc
     3601 cggccttgct gtcgcgcagc tcgatgctcg cggcgtcgaa agtgggatcg aattgccggg
     3661 cgatggtggt gaccgcgtga acgaagccgt cgacttttgc gcccatcacc ttgatgggga
     3721 agcgggatgg atactggatc agggactgct tgggatcttg cggcgcggaa ggcggcactg
     3781 cgggtttgct cattcaactg ctccgccgcg tcgcacgata cgcggcatat ggactagtac
     3841 ggccgaggcg cgggccaccg cggaaccggc tttgccggtc cgctggtggc gccccctcga
     3901 gggggagagg gcaaagcccg agggggcatc ggccgcaggc cgcttcgggg gtgatcattt
     3961 ctctttggca cgctggtagc cggcgtacaa ctgctcgtag atggggccgg ggcggccggt
     4021 tccaatagtt tgctggtcca gccgcgtcac gggcaggact tccttggtgg cgcttgacag
     4081 catgagctca tcggcggcga acacttcatc tcgcgaaatg gggcgcagct ggaacgggat
     4141 gccggcttgc ccgcacagtt cttcgatcag cccgtagcgg atgccttcga gcacgaggtt
     4201 gcttttcggc gcgcccatca ccgtgccgtt gctgaccacc cacacgttgc aggccgcagc
     4261 ctcgctgagc cagccatcgc ggaacatgac cgtttcggca gcgcccacgt cggcgctcat
     4321 ctggcgcgcc agcaccgcgc cggccaggct gatgctcttg atgtgcgctt ttttccagcg
     4381 gaagtcctcg ccgctcacac acgccacgcc ggccgcgcgg tcctgcgccg aaggtgggct
     4441 catccggttg ctgctggcga acaccgtggg cgtgatgtcc ggcggcatga catggtcgcg
     4501 cattgcaacg ccccgcgtga cctgcagata caccaattga tcggtttcac ctgggttttt
     4561 gcccagcgtc ttcgcgtacg ccgcgaccag ccggtcgacc agttcgcgcc attgcgccgg
     4621 gtccatcgga ttggtgatgc gcaattccaa caggctgcgg gccagccgcg ccatgtgctg
     4681 cgcaaacctg aacggccgtc ccgcatagac cggcaccacc tcgtacacgc cgtcgccgaa
     4741 gatgaagccg cggtccatga cgctgacctt cgcctcgcgc aaggtggtga attcgccgtt
     4801 gaggtaacag gggagatcgg ggagttggta tgggttcatg ctgctgattg tccagtgcgc
     4861 gcattgtgct gtggctcgaa aaacacctcc gtcatgggac catagcccga cggcacggtc
     4921 aggttcgtga aaatgacccg gggtttctac ttataattaa gggctttgtg aaagtttcgg
     4981 ggccgaaagc gggctggaca gctatgaaaa aaatcgcgcc gctgcctgaa acgacggagg
     5041 aagagccccc tttcaagccg ctgaccgccg aagaggcgcg ccggctgcgc gaagaacacc
     5101 ccctggtttc cccctggtgg gtggtggcgg ggcagatcgt ggtgggagtc gtggtggcgc
     5161 tggcagcctg ggcgctcacg gggagacaaa acgttggatg gtccgccgga tatggcgcgc
     5221 tggtcgtggt gattcccgcg gccatttttg cgcggggcct gaccgggcgt ttttcttccc
     5281 tgaacgcggt gacggcggct gtcggcttcc tggtgtggga gatggtcaag atcgcctcgt
     5341 cgatcgccct gttggcggcg gcgccgaggc tggtgccaga actgagctgg ccggcattgc
     5401 tggtggggct ggtgctgacg ataaaggtgt attgggtcgc attggcgtat tcgccccgca
     5461 agggtcgcgc cgcaaacgtc aaactttgaa ctgaagaaac atggctgctg aacacggacc
     5521 gaccgcgggt gaatacatca ttcaccacct gaagcacctg cagaaccaca aggcgaccaa
     5581 cgtcgtcgat ttctccgtct tcaatttcga ttcgatgttc ttctccacag cgctgggcat
     5641 cctggtctgc tgggtgctgt ggctggcagc gcgcaaggcc acctcgggcg tgccaggacg
     5701 cttccaggcc gccgtcgaga tcctggtcga gatggtggac acgcaggcca agggcatcgt
     5761 ccacaacgcg cagagccgca agttcgtcgc cccgctggcg ctcacggtgt tcatctggat
     5821 cttcatgctc aatgcgatgg acctgcttcc ggtggacctg cttccctgga tctgggaacg
     5881 catctacgct gccaccggca atgacccgca cgaggcctac ctgcgcgtcg tgcccaccgc
     5941 cgacctgtcc atgaccatgg gcctgtcggt gtcggtgctg ctgatctgcc tgttctacaa
     6001 catcaagatc aagggcctgg gcggctgggt ccacgagctg ttcacggcgc cgttcggggc
     6061 gcacccggcg ttgtggccgt tcaacttcgc catgcagatg atcgaattca tcgccaaaac
     6121 cgtgtcgcac ggcatgcggc tgttcggcaa catgtatgcc ggcgaactga tcttcctgct
     6181 gatcgccctg atgggcggcg cctggtcgct gtcggccacc ggaatcggcc tggccatcgg
     6241 ccacatcatt gccggcaccg cctgggcaat cttccacatc ctgatcatca cgctgcaggc
     6301 attcgtgttc atgatgctgg cgctggtgta cctgggccag gcgcacgacg cccactaaag
     6361 agcagttttt cgttttccgt ctttccttat caacgtcaac caagtcccct aggagcaatc
     6421 atggaagtta tcagctttgt cgcactggcc accggcctga tcatcggtct gggcgccatc
     6481 ggcgcctgta tcggcatcgg catcatgggc agcaagtacc tcgaatccgc tgcccggcaa
     6541 cccgagctga tgggcgagct gcagaccaag atgttcctgc tggtcggcct gatcgacgcc
     6601 tcgttcatca tcggcgtcgg catcgcgctg tggttcgcca ccgccaaccc gttcctggcc
     6661 cagatcgcca acctgccgaa gtaagcgtcg aagaaaacgt catgccccgt cgccagcgcg
     6721 ccgagggtga aggatgaaaa agtgagcatt accggtaccc tgatcattca gatgatcgtg
     6781 ttcctgatcc tggtcgggtt cacgatgaag ttcgtgtggc cgccgatcgc cgccgcgctc
     6841 gacgagcgcg cgcgcaagat tgccgacggg ctggctgccg ccgacaaggc caaggccgag
     6901 ctggcagccg ccgaccggcg cgtggaagaa gagctgggca agacgcgcac cgagaccgcc
     6961 gcgctgctgg ccgacgccga gcgccggggc cagcagatcg tcgaggaagc caaggcccgt
     7021 gcgctggagg aagccaacaa gatcatcgcg gccgccaagg ttgaggccga tcagcaaagc
     7081 gtcaaggcac gggaagccct gcgcgagcag gtggccgcgc tggccgtcaa gggcgccgag
     7141 cagatcctgc gcaaggaagt caatgcttcg gtccatgcgg agctcctcga gcgcctgaaa
     7201 accgagctgt agaggatcac catggctgaa ctcgccacca tcgcgcgtcc ttacgccgaa
     7261 gccttgtaca agtccgcccg cagcgacctg aacggagcga gcctttggct cgatgcgctg
     7321 gccgcggttg ccggcaatgc gcagctactg cagttcgcca acagccccaa ggtcaccgac
     7381 cagcaggtgt tcgatgtgat ggccgacgtg gccaaggaac ccctgcccga gaacggcaag
     7441 aacttcctg
//
LOCUS       DASWIY010000409         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_650811, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000409 DASWIY010000000
VERSION     DASWIY010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650811"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VGA59_14370"
     CDS             complement(<1..375)
                     /locus_tag="VGA59_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX9720906.1"
                     /translation="MSVVAVVGLGYVGLPLAVEFGKKGKTIGYDLSQTKVESYRRFID
                     PTCEVSSEDLRAAAQLAVTTDPVALAQADFIIIAVPTPVDGAHQPDLSALSGASESVG
                     RHMKRGAIVVYESTVYPGATEET"
     gene            complement(393..1241)
                     /locus_tag="VGA59_14375"
     CDS             complement(393..1241)
                     /locus_tag="VGA59_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system-associated ATPase"
                     /protein_id="HEX9720907.1"
                     /translation="MYEAFYHLQAKPFQLSPDPRFFYSSSGHRRAMAYLVYGVHQGEG
                     FIVITGEIGAGKTMLANTLARRLASGNLVLAQVVSTQLKDDDMVRMVAAAFGVHQDAS
                     KAVLLNRVEQFLLVCHRHGKRALLIVDEAQNLPASSVEELRMLSNYVSAAKPLLQTFL
                     LGQPEFRKTLQSPEMEQLRQRIIASCHLGPLDSAETEAYIVHRLQTAGWQGDPSFSAD
                     AFTAIYQHTGGIPRKINIMCDRLLLLGRLDELHAFTGSEVAKVIEELPQEFAPPGPRV
                     SQEPRG"
     gene            complement(1248..>1531)
                     /locus_tag="VGA59_14380"
     CDS             complement(1248..>1531)
                     /locus_tag="VGA59_14380"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9720908.1"
                     /translation="AASNTIRQTGAGLAWNWRVTPWTTWNLGGSYGRNEFLDSDRVDH
                     LTFIRAGITRQFQPRLSGSLYYRRQQNDSNEIGSSYTENAGIAALQMRF"
BASE COUNT          280 a          472 c          475 g          304 t
ORIGIN      
        1 tgtctcctcc gtagcgccgg gatagacggt ggattcgtag acgacgatcg cgccgcgttt
       61 catgtgcctg ccaaccgact cgctggcgcc ggagagcgcg gaaagatcgg gctggtgtgc
      121 gccatccacc ggcgtcggca ccgcgataat gatgaaatcg gcttgcgcga gtgccaccgg
      181 atcggtggtc acagcgagct gggcagcagc gcgcaagtcc tcgctcgaca cttcgcaggt
      241 cggatcgatg aacctgcgat agctttcaac ctttgtttgg gacaggtcat agccgatggt
      301 tttgcccttc ttgccaaact ccacggcgag cggcaagccc acgtatccca gtccaacgac
      361 ggcgacaacg ctcatattcc tccgaactgg aatcagcctc ttggttcctg gctgacccgc
      421 ggacccggtg gcgcgaattc ctgcggcaac tcctcgatga ccttggcaac ttcgctgccg
      481 gtaaacgcat gcagttcatc caggcggccc aggagcaaca ggcggtcgca catgatgttg
      541 atcttgcgcg gaatgccgcc ggtgtgctga tagatggcag taaacgcatc cgcgctgaac
      601 gatgggtcgc cctgccagcc tgcggtctgc agccggtgca cgatataggc ctcggtctcc
      661 gcgctgtcca gcggcccgag gtggcacgag gcgatgatcc gttgcctgag ctgctccatc
      721 tcggggctct gcaaggtctt gcggaactcc ggctgcccaa gcaggaaagt ctgaagcaag
      781 ggcttggcgg cggacacgta gttcgacagc atgcgcaact cctcgaccga tgacgccggg
      841 aggttttgcg cttcgtcgac gatcaggagc gctcgcttgc cgtgccggtg gcacacgagc
      901 agaaattgct ctacgcggtt gagcagcacc gccttgctgg cgtcctggtg cacgccgaat
      961 gccgcggcca ccatgcgcac catgtcgtcg tccttcagct gggtactcac gacttgtgcc
     1021 agcacgaggt tccccgaggc gagcctgcgg gcaagagtat tcgcgagcat ggtcttgcct
     1081 gcgccgatct ctcccgtgat gactatgaat ccctcgcctt ggtgcacgcc atacaccaga
     1141 tacgccatgg cgcgccggtg accgctgctg gagtagaaga agcgcggatc cgggctcagc
     1201 tgaaacggct tggcctggag atgatagaaa gcttcgtaca ttgagcgtca gaatctcatc
     1261 tggagggcgg ctatcccggc gttttccgtg tagctggatc cgatctcgtt agaatcattc
     1321 tgttgcctgc gatagtacaa ggacccggaa agccgcggct ggaactgccg ggttattccc
     1381 gcccgaataa acgtgaggtg gtccacccgg tcgctgtcga ggaattcgtt acggccgtag
     1441 ctcccaccca gattccaggt ggtccatggc gtcacgcgcc aattccacgc aaggccggcc
     1501 ccggtctgcc ggatcgtgtt gctcgcggcg a
//
LOCUS       DASWIY010000410         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1030408, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000410 DASWIY010000000
VERSION     DASWIY010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030408"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VGA59_14385"
     CDS             <1..677
                     /locus_tag="VGA59_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9720909.1"
                     /translation="VNDSRGFFTSRVFGTFVMEGAAMVGEGIPAAVVEQAGIQAGMPV
                     GPLAVLDETALSLSAHVMDQTRADYRAEGGTYIATPGELLVERMVKEFDRNGRAAGAG
                     FYEYPEEKGAKKYLWPELKTLFEKPGLEWNMQDLKDRLLYRQAVETARCLCENVLTTV
                     HDANIGSIFGIGFPAWTGGAMQFIYGTGIDAFCDRAAQLADRYGPGFVVTAQVRDAIR
                     KHQPIY"
     gene            825..2057
                     /locus_tag="VGA59_14390"
     CDS             825..2057
                     /locus_tag="VGA59_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9720910.1"
                     /translation="MTNAAALTNVQRVLIAKGLRSFGDGFVSLLLPAYLLALGFTPLQ
                     VGVIATTTLLGSGILTLAVGLQAWRFHYRTWLLASTLLMAGTGLGFALLTDFWPLMLI
                     ALVGTLNPSSGDVSVFLPLEQSLLSRIVSDKRRTWVMARYSLVGSLLAAFGSLAAALP
                     ALAAASAGIAMISALQGMFLLYTLLACAAAAVYRGLPRAEASDAPQPKAALHQSKKNV
                     YTLAALFSLDAFGGGFVVQSLVALWLYQRFGLSLSAASAIFFWTGVLTAFSYLVAARI
                     AGRIGLLKTMVFTHLPSSVCLILIPFMPELGYAIALLFLRSALSQMDVPTRSSYVMAV
                     VPPAERAAAASVTSVPRSLASAASPVLAGYLLGLSPFGWAFVAGGVIKIVYDLLLLAM
                     FHKVRPPEEQPERLSSRT"
BASE COUNT          323 a          749 c          705 g          424 t
ORIGIN      
        1 tggtcaacga ttcgcgcggc ttcttcacgt cgcgggtctt cggcaccttc gtgatggaag
       61 gcgcggcgat ggtcggcgaa ggcattccgg cggccgtcgt ggaacaggcg ggcatccagg
      121 ccggcatgcc ggtgggcccg ctcgcggtgc tggacgaaac ggccctgtcg ctgtcggcgc
      181 acgtgatgga ccagacccgc gccgactacc gcgccgaggg tggcacctac atcgccacgc
      241 ccggcgagtt gctggtcgag cgcatggtca aggagttcga tcgcaatggc cgcgctgcgg
      301 gcgccggctt ctacgaatac cccgaggaga aaggcgcgaa gaagtacctc tggccggagc
      361 tcaagacctt gttcgaaaag cccggtctcg aatggaacat gcaggacctg aaggaccggc
      421 tgctctaccg ccaggccgtg gaaaccgcac gctgcctctg cgaaaacgtg ctgaccacgg
      481 tgcacgacgc gaacatcggc tcgatcttcg gcatcggctt tcccgcctgg accggtggcg
      541 ccatgcagtt catctacggc accggcatcg acgccttctg cgaccgggca gctcaactgg
      601 cggaccggta cggacctggc ttcgtggtca cggcgcaggt ccgggacgcg atccgcaagc
      661 accagcccat ttattgagcg tggtgcgaat cgccgagctc acggaaccca taagggacta
      721 gaatttccgt caccggggga acgagcagac tccccgctgc caaagacgct ccgcgtccaa
      781 gcgctgctcc tgatcatggg actatgccca gttaggagcc tgacttgacc aacgccgccg
      841 ccctcaccaa tgtccaacgt gtcctgatcg ccaagggcct gcgctccttc ggggacggct
      901 tcgtcagcct gctgttgccg gcttatctgc tggcgctcgg attcacgccg ctgcaagtcg
      961 gcgtgatcgc caccacgaca ctgctggggt ccggcattct gacgctggcc gtgggcttgc
     1021 aggcctggcg cttccactac cgcacctggc tgctcgcatc gacgctgctg atggcgggaa
     1081 ccggcctcgg cttcgcactg ctgacggact tctggcccct gatgctgatc gccctggtcg
     1141 gcacactcaa tccctccagc ggcgacgtca gcgtgttcct tccgctggag caatccttgc
     1201 tctcacggat cgtgtcggac aagcgccgca cctgggtgat ggcccgctac agcctggtcg
     1261 gctcgctgct tgcggccttc gggtccctcg ccgcggcgtt gccggcgctg gccgccgctt
     1321 ccgcgggcat cgccatgatc tcagcgctcc agggcatgtt cttgctctat accctgctcg
     1381 cctgcgcggc ggcagcggtg taccgcggcc tgcccagggc cgaggcgagc gacgcgccac
     1441 agccgaaggc cgcgttgcat caatcgaaga agaacgtcta cacgctcgcg gccctgttca
     1501 gcctcgatgc attcggcggc ggcttcgtgg tgcagtcgct ggtcgcgctg tggctgtacc
     1561 agcggttcgg gctgtcgctg tcggcggcga gtgcgatctt tttctggacc ggcgtgctca
     1621 cggcattttc gtatctggtc gccgcgcgca tcgccggccg catcggcctg ctcaagacca
     1681 tggtgttcac ccacctgcct tccagcgtct gcctgatcct tatccctttc atgcccgagc
     1741 tgggctatgc gatcgcgctc cttttcctgc gcagcgcgtt gtcgcaaatg gatgtgccca
     1801 cccgaagctc gtatgtgatg gcggtcgttc cgccggcgga gcgcgccgcc gccgccagtg
     1861 tcacttcggt gccgcgcagc ctggcctcgg ccgccagccc cgtgctggcg ggctacctgc
     1921 tgggcctgtc gccgttcggc tgggccttcg tagcgggcgg cgtcatcaag atcgtgtacg
     1981 acctgctgct gctggccatg ttccacaagg tccgcccgcc ggaggagcag cctgaacggt
     2041 tgtcatcccg gacttgacgc ccggttgtca ccccgggcct gacccggggt ccatgacttc
     2101 ctggattgcg gatcgggcct gcttcggacc ccggatcaag tccggggcag gcttgatccg
     2161 gggtccgcaa tgacagcgtg tagttacttg actttggcaa c
//
LOCUS       DASWIY010000411        17777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1782867, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000411 DASWIY010000000
VERSION     DASWIY010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 17777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17777
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782867"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VGA59_14395"
     CDS             complement(<1..884)
                     /locus_tag="VGA59_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HEX9720911.1"
                     /translation="MARIVKVGAAQLGPIQRGDTREAVVRRLLELLREAHAMACDVVV
                     FPELALTTFFPRWYMEDQAEVDGFFEREMPGPMTRPLFDEAARLGIGFYLGYAELTQE
                     EGRTRRFNTAILVDKTGKIAGKYRKIHLPGHAEHEPWRRFQHLEKRYFETGNLGFNVS
                     RAYGGIMGMCICNDRRWPETYRVMGLQGVEMIMLGYNTPVHNPPAPVHDNLSHFHNQL
                     VMQSGAYQNGTWVIGVAKAGCEEGCDMIGNSSIIAPSGEIVGSCTTLEDELAVARCDL
                     DLCNSFKNSVFNFAVHRQP"
     gene            complement(1052..2302)
                     /locus_tag="VGA59_14400"
     CDS             complement(1052..2302)
                     /locus_tag="VGA59_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX9720912.1"
                     /translation="MNDIASVSVRDLRIDANRLGARIDALGAVGAIPEGGVCRLALTA
                     EDRAGRDLVVGWMRELGLAVTIDAIGNVVGMRQGIESGPPVMCGSHIDTVRTGGRFDG
                     NLGVLAGLEAIQTLADANIVTRRPLAVAFFTNEEGARFNPDMMGSLVYVGGLPLEKAL
                     GTVGIDGESVGDCLTRIGYAGTAPCGNSQVHAYVELHVEQGPVLDIEGITIGAVEGVQ
                     GISWTEFTVAGVSNHAGTTPMRLRHDAGYVACAIAHQAREIAGSLGGDQVATVGAINL
                     SPNLVNVIANKAVFTVDLRNTDESVLQEAERRLHASAGRIAAAEGVTLSHRTLARFEP
                     VAFDPAVVQLVEATARRLGHSVRRLPSGAGHDAQMLARICPAGMIFVPSVGGISHNVK
                     EFTALPDLEAGANVLLHTLLALAK"
     gene            complement(2302..3339)
                     /locus_tag="VGA59_14405"
     CDS             complement(2302..3339)
                     /locus_tag="VGA59_14405"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX9720913.1"
                     /translation="MRLFKSIAISALLAVSVGMMPTAALAQKTIKLAHLNKNDPFDNG
                     TGAMAIVFKSLVESGTNGAVKVEIFPDGQLGKDNEVIQQVKSGVVQSSISSVGGIASV
                     YPMIGVLDVPFAYPNIAVTYEVFDGPFGRKLSADITKKTGLEVLGFGDSGGFFAFTNS
                     KRPIKSPEDMKGLKIRTMGLESHKAVVSSMGGQPVAINWAEVYTSLQTGVADGQMNPI
                     PIVKFAKFDEVQKYLTLTNHLFTPYVWIFNKGALDGLSAQEKEVVKSAAKSAIIACRG
                     INRIIEASDRGLPALAQKMQVYTPTKAEIAKFRDLAQPAVKAQIAKSYGKEGEALMAD
                     FLAAIDKAAKN"
     gene            complement(3396..4679)
                     /locus_tag="VGA59_14410"
     CDS             complement(3396..4679)
                     /locus_tag="VGA59_14410"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9720914.1"
                     /translation="MMLWVLATFFVLLAVGVPIGYVLGIAGVLGILQIGGAEMLAMAP
                     QRYFAGLDLFTFLAMPLFIFAGELMNKAGITDRLAKFADALVGHLRGGMAQSCMVSSV
                     LFAGITGAAVAEAAAFGSTMVPAMEKNGYKKPFACGVVVAGSIIGPTIPPSNLMVIYG
                     SMMGVSIASLFAAGILPGLVMGLVCMSVIAGLGRRLNLPKGGQRQSLLGILLAFKNSL
                     SALVMPVIILGGIIGGFVTPTEGAAIAVFYALVLGVFVFRSVKLQDIVGMLVRTARIT
                     GVVFLIIAAASILSWWMTYMKLPQLITGLFLGLTSDPSMIILMVLVLLLILGMFMDIN
                     AILIVLGPILGALAVSIGMNPVQAGIMIVLALNIGLMTPPVGASLFVLSSITGAKIED
                     ISKAMWPFVIAEVAVLILVAYWPSLTLTMPRLMGL"
     gene            complement(4676..5218)
                     /locus_tag="VGA59_14415"
     CDS             complement(4676..5218)
                     /locus_tag="VGA59_14415"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9720915.1"
                     /translation="MEISPELPPMQRWGGRSAALSGKINRWIELLCAGLLAILVIDIW
                     IGVFGRYVFELPVTWAEELARYLMIWTALLAVSCGVARREHVAVTALLHRLPIRIRRW
                     FEVAIDLLAFAFFAFLFYFGIGMTQQGASQYATIFDMTMAIPFASVPASSALVCLQLV
                     LMGIRDFTVAGLPADFAENE"
     gene            complement(5241..6098)
                     /locus_tag="VGA59_14420"
     CDS             complement(5241..6098)
                     /locus_tag="VGA59_14420"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX9720916.1"
                     /translation="MQQLEPTIQESIMAAYESLPPGERKLADTVLSRLGNLASYSATE
                     LAADASVSKATAARFFRRIGYESFEHARRRARAESHLASPLYALAGVDAKERRSDALS
                     RHLAADLKNLSESFRQSDSAVLEQAVAQTAKAPRVHVVGMRNGHFVAAYAAYLLAQVR
                     EDVVSLPTAAMTLAEDLASLRRGDVLLVVDFRRRATLLQTIVDAARQAGARLVFIASP
                     GMPPLSRAGDVVVHCLTGGASIFDSYGAAISVVNYLCAAVARELGRKSRKRLEAIEDL
                     HASIGDIRH"
     gene            6387..7424
                     /locus_tag="VGA59_14425"
     CDS             6387..7424
                     /locus_tag="VGA59_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9720917.1"
                     /translation="MKKRVFLQLAAALGLACGFGAVQAQTTPIKFQLDWRFEGPAALF
                     LTPAARGYFKDAKLDVTIDAGTGSGVAVTRVASGTYDMGFADMAALMEFHANNPDAPN
                     KPVAVMVVYNNTPASVMALKKSGIRTPADLNGKKLGAPVFDAGRRAFPIFQKANGVGS
                     VAWTAMDPPLRETMLVRGDVDAITGFTFTSLLNLEARGVKAEDVVVMPYASHGVKLYG
                     NVVIASPKIIKENPAAVKAFLAALTKGTKEVLSRPEAAVADVKARDAIINSGMEIRRL
                     KLAIDTVINSSDARAEGFGQINPARLTLMASQVSDAFNTKTRVNPDAVWNGSFLPPKA
                     DLNAVLPAPRK"
     gene            7421..8245
                     /locus_tag="VGA59_14430"
     CDS             7421..8245
                     /locus_tag="VGA59_14430"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9720918.1"
                     /translation="MTEGPFVDFQDVWLSYNEELLRQGQYAVEAIDLTVGSGEFIAIV
                     GPSGCGKSTFMKLATGLKMPSKGRILVEGQPVTGPLKISGMAFQAPSLLPWRTTVDNV
                     LLPLEIVEPYRSNFKSRRKEYEQRARLLLQKVGLGGYEDQYPWQLSGGMQQRASICRA
                     LIHEPKMLLLDEPFGALDAFTREELWCILRDLWTEQRFNVILVTHDLRESVFLADTVY
                     VMSQRPGRFMVRKEVDLPRPRDLEITYTRQFIDLVHELRGHIGAVREQAAAAAAVS"
     gene            8242..9009
                     /locus_tag="VGA59_14435"
     CDS             8242..9009
                     /locus_tag="VGA59_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9720919.1"
                     /translation="MKTTHLERWSPWILLVAVIGLWQIVCSVFNVSEFIFPSPARIWQ
                     QLVEFRGAIAVHAWRTLWVTVAGFGLAIVVGVLLGFLIGSSRIAYAAVYPLMTAFNAL
                     PKAAFVPILVVWFGIGAGPAILTAFLISFFPIMVNIATGLATLEPELEDVLRVLGARR
                     WDVLVKVGLPRSMPYFYGSLKVAITLAFVGTTVSEMTAANEGIGYLLISAGSSMQMGL
                     AFAGLVVVGAMAMLMYELFSWLETHTTAWAHRGSQAR"
     gene            complement(9016..10419)
                     /gene="hydA"
                     /locus_tag="VGA59_14440"
     CDS             complement(9016..10419)
                     /gene="hydA"
                     /locus_tag="VGA59_14440"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX9720920.1"
                     /translation="MTDTLIRGGTLVTADGRRRADLLCRDGLIAAIGTGLELQAGTEV
                     IEAGGCFVMPGGIDPHTHLQLPMMGTVVADDFYTGTAAAAAGGTTSFLDFVGPERGQS
                     PLEALAIWKSRAAKAAIDYGFHMTVSWWGPRFSEEMGALVRDHGITSFKFFLAYKGGL
                     MLPDDELIAGFRRCRELGALPQVHAENGELIAYLQAKMLAEGVTSPLGHVLSRPPQCE
                     GEATGRAITMAEIIDVPLYVVHVSAAEAARAIAQARGRGVKVIGETLPGFLAIDDSVY
                     RHADFDFAAGHVMSPPYRPREHQEVLWQALREGALSTTGTDHCCFTKAQKRLGQDDFT
                     RIPNGCGGIEDRLHVLWHLGVNGGRISPEQFVALTSTNAARTFGLWPRKGGLEIGADA
                     DVIVLDPAGTRTLSAATQRQNTDFSVWEGMEVRGVVVHTFSRGAHLWADGDLRAESGR
                     GRYLGRLPFGHPYQGRL"
     gene            complement(10416..12479)
                     /locus_tag="VGA59_14445"
     CDS             complement(10416..12479)
                     /locus_tag="VGA59_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720921.1"
                     /translation="MAELIPIPFANLATRLFSELEQKKAAFDLPERFFYRGSNTRDLS
                     LNLHGGRASTPFGPAAGPHTQMAQNIVLAWLCGGRFMELKTVQIKDDLHIPRPCIDMQ
                     TVGFNVEWSQELKLAQSLEEYVKASMLIEMLRASGMAAGCTDTVFDMSVGYDLAGIGS
                     PPVRGFLAGLMDAGAVVERLRAQIPPALARFRDLPFRTRISDTLTLSTFHGCPPGEIE
                     AIAAFLLREMDLNVVVKLNPTLLGRESLLEILHGRLGYTELMVPDAAFENDPKWEQVT
                     AIVNRLGDLAARLGRGFGVKFSNTLLVKNHKAFFPATESQMYMSGLPLHVLAMVLVQR
                     FRSVFGDRFPVSFSAGIDDGNFADAVALGLKPVTVCSDLLKAGGYGRAIKYLAALEER
                     MDAVGARGIDEYIVRALGHGQAALKDLGLPGERAEACRSALEGKGDLAAAADADFPAW
                     VSACVLRNTNTYVERLLEDPRYSRAEHAAAPRKIASRLALFDCTIACNKCLPVCPNDA
                     NFPFVIPPGSYPVERLVPTPGGWRSEPAGALVFARHRQLGNFADACNECGNCDVFCPE
                     DGGPYLAKARFFGSAAAWKETPDRDGFAFALDGDTLTMLGRFEGQEVRVETTPGSKLR
                     YAGQGFDIRLDLDDPLGSVEGQATAAVDLRRLRMMDLIRTAVASPQANNFISTGLLLA
                     ATPTP"
     gene            12647..15286
                     /gene="xdh"
                     /locus_tag="VGA59_14450"
     CDS             12647..15286
                     /gene="xdh"
                     /locus_tag="VGA59_14450"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HEX9720922.1"
                     /translation="MNLYQFSLNGTPVEVHAPPDRLLLEVLRENFKIASVKNGCAPQQ
                     ACGACLILINGVPKNSCALDMEKMQGKEVLTLEGVGEDERQLFARAFQAAAGLQCGFC
                     TPGLVLRIKWLADRGRALSRPEIAKALTGHLCRCTGYVKIIDAVELILKAKRGGQLPE
                     LLEGGRVGQSLPRYQGLELALGSRPFVADIEVPGLLHGAVVLSPHAHARVLGIDTGKA
                     AALSGVVKVITAKDVPGDRWVGQICKDWPVLVAEGEEVRYVGDVLAVVAAQTPHIARA
                     AAGLVAVAYEPLPPLLDPAEAIKPSAPQVNPRHDNVLSRTSIQRGAVDAALVSSAHVV
                     SGTWRTQRIEHLFLEPEAALAVPLQDGRLHLLTQGQGVFDDRRQVASVLGESENKVFV
                     ELVPNGGAFGGKEDMTIQAQTALAARLTGRPVRIELNREESIRMHPKRHPITMTYTVG
                     CDAQGRLTAARIYLLGDSGAYASVGGKVLERAAGHACGPYRIPAIDIEAVAAYTNNPP
                     CGAMRGFGVNQTSFAIEGCLDLLAKKVGIDGWEMRWRNAVRVGDVFSSGQVLDKSIGL
                     ERTLLAVKDTYYAARSSGRAVGLACGVKNSGIGNGAIEKGKCRLQVEADASVSLYSGF
                     TEMGQGLFTVLIQFAVEVTGLAPSVFRPKVTSRFELGSGQTTGSRATLLAGRATIDAA
                     GKLSVDLKAGHTLTDLAGRIYEGETTIDDTTAPGQTKNGIIKTHTSFGWSSQVVVLDE
                     RGAVELVHVAQDVGRAINPQQCEAQIEGAVAMGLGYALTEELPCKDGMPVTFHPREIG
                     VLRAPHMPRVEVTLIEEHEPEGPFGAKGIGELGLVPTAGAVAAALEAFDGVRRMTLPM
                     KDSAAARAIDVGLIKDGDRDQWH"
     gene            15277..16467
                     /locus_tag="VGA59_14455"
     CDS             15277..16467
                     /locus_tag="VGA59_14455"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9720923.1"
                     /translation="MALTPPLRIGPDRLGRSVAVADGLVVATAPTDAIRLPCAQGEIA
                     AGAVCAHTHLYSGLVRYGMPPATPAPETFLKILERVWWRLDRVLDAETLRAAAMDYVA
                     RALLAGTTTLVDHHESPNLIEGSLEILAEVCGQLGVRALLCYGATERNFGAAEARRGL
                     DECRRVPASPLLRGLVGLHASFTVSDDTIRAAARLAEDLGTVVHVHVAEDAADVADAL
                     ARGYPGPLERLDRLGALPRGSILAHGVHLDGEQARHCDELGCWLVHNPRSNEGNRVGY
                     ALNLGCSNRVALGCDGWDADMTTEQEALSRLAGIHGDVKAQGRLANGHVLIAERFASH
                     PEPLQPGALGDVVVRQDGAVRHVVVGGRVVVADGVLATGDFDSITAGARKQAGRLWQR
                     MANT"
     gene            16544..17623
                     /locus_tag="VGA59_14460"
                     /pseudo
     CDS             16544..17623
                     /locus_tag="VGA59_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         3289 a         5619 c         5755 g         3114 t
ORIGIN      
        1 ggctggcgat gcaccgcgaa attgaaaacg ctgttcttga acgaattgca caagtcgagg
       61 tcgcagcgcg ccactgccaa ttcgtcttcc agtgtggtgc aggagccgac gatctcgccc
      121 gagggcgcga tgatcgagct gttgccgatc atgtcgcaac cttcctcgca gccggccttg
      181 gccaccccga tcacccaggt gccgttctgg taggcgcccg actgcatgac cagctggttg
      241 tggaagtgcg acaggttgtc atgcaccgga gccggcgggt tgtgcaccgg cgtgttgtag
      301 ccaagcatga tcatctcgac gccttgcagc cccatgaccc gatacgtctc cggccagcgg
      361 cggtcgttgc agatgcacat gcccatgatg ccgccgtacg cgcggctcac gttgaaaccg
      421 aggttgccgg tttcgaagta gcgtttctcc aggtgctgga aacggcgcca cggctcatgc
      481 tcggcgtggc ccggtaaatg gatcttgcga tatttgccgg cgatcttgcc ggtcttgtcc
      541 acgaggatgg ccgtattgaa gcggcgggtg cggccttcct cttgcgtcag ttcggcgtag
      601 cccagataaa aaccgatgcc caggcgcgcg gcttcgtcga acaaggggcg cgtcatcggc
      661 ccgggcatct cgcgctcgaa gaaaccgtcg acctcggcct ggtcttccat gtaccagcgc
      721 gggaaaaacg tggtcagcgc cagctcgggg aacacgacga catcgcacgc catggcgtgc
      781 gcctcgcgca gtaattccag caagcggcgc acgacagcct cgcgcgtgtc gccgcgctgg
      841 atcgggccca gctgcgccgc ccccactttg actatccttg ccatacgctc atctcctggt
      901 cagttcagtt ttccacggca acgctgcgcg ctacccgtag cctatgaaat cgcacggccg
      961 ctgcgggggc caccgcggaa ccggctttgc cgggccgctg gtggcgcccc cttgaggggg
     1021 aggcgccgaa ggcgcttcgg gggtgggctt ttcatttcgc caacgccagc agggtgtgca
     1081 gcagcacgtt cgctccggcc tcgagatccg gcaatgcggt gaattccttc acgttgtggc
     1141 tgatgccacc gacgctcggg acgaagatca tcccggccgg acaaatccgg gccagcatct
     1201 gcgcgtcgtg cccggcaccg ctgggcaagc ggcgcaccga atggccgagg cgtcgcgcgg
     1261 tcgcttcgac cagttggacg acggcggggt cgaaggccac cggctcgaaa cgcgccagcg
     1321 tgcggtggct cagcgtgaca ccttccgccg ccgcgatccg gccggccgac gcatgcaagc
     1381 ggcgctcggc ttcctgcaac acggattcgt cggtattgcg caagtcgacc gtgaataccg
     1441 ccttgttggc aatgacattc acgagattcg gcgacaggtt gatggcaccg acggtggcca
     1501 cctgatcgcc acccagcgaa ccggcgatct cgcgcgcctg atgggcgatg gcgcaggcca
     1561 cgtagccggc gtcgtggcgc aggcgcatcg gcgtggttcc ggcgtggttc gacaccccgg
     1621 ccacggtgaa ctccgtccag gaaataccct gtacgccttc cacggcgccg atcgtgattc
     1681 cttctatatc caggacaggg ccctgctcga cgtgcagctc cacgtaggca tgcacttgag
     1741 agttgccgca gggtgccgtc cccgcatagc cgatcctggt caggcagtcg ccgacggact
     1801 cgccatcgat gccgaccgtg cccagcgctt tctcgagggg caaaccgccg acatagacaa
     1861 ggctgcccat catgtcggga ttgaagcgcg cgccttcctc gttggtgaaa aaggccacgg
     1921 ccagcggccg gcgcgtcacg atgttcgcat cggccagtgt ctggatggct tccagtcccg
     1981 ccagcacgcc gagattgccg tcgaagcgtc cgccggtgcg gacggtgtcg atgtgagacc
     2041 cgcacatgac cggcgggccg gactcgatgc cctggcgcat gccgacgaca ttgccgatcg
     2101 catcgatcgt gacggcgagc cccagctcac gcatccagcc gacgaccagg tcgcgccccg
     2161 cgcgatcttc ggccgtcagc gcgagccggc agaccccgcc ttcgggaatc gcgccaacgg
     2221 cgccgagcgc atcgatacgg gcccccaggc ggttcgcatc gatgcgcaag tctcgcacgc
     2281 tgacgctcgc gatgtcgttc atcagttctt cgcagccttg tcgatcgccg ccaggaagtc
     2341 agccatcagg gcttcgccct ccttgccgta gctcttggcg atctgggcct tgacggccgg
     2401 ttgagccaaa tcgcggaact tggcgatttc agccttggtc ggggtataga cctgcatctt
     2461 ttgcgccagc gccggcaagc cgcggtcaga agcttcgatg atgcggttga tgccgcggca
     2521 ggcgatgatg gccgatttgg cagccgactt gacgacttcc ttttcctggg ccgaaagacc
     2581 atcgagcgcg cccttgttga agatccacac atacggcgtg aacaggtggt tcgtcagggt
     2641 caggtacttc tggacttcgt cgaacttggc gaacttgacg atcgggatgg ggttcatctg
     2701 gccatcggca acaccggtct gcagggacgt atagacctcc gcccagttga tcgccaccgg
     2761 ctggccgccc atgctgctga cgaccgcctt gtgcgattcg agtcccatcg tgcggatctt
     2821 caggcccttc atgtcttcag gcgacttgat cgggcgcttg gagttggtga aagcgaagaa
     2881 accacccgag tcgccaaagc ccagcacttc caggccggtc ttcttggtga tgtcggcaga
     2941 aagtttgcgg ccgaaggggc cgtcgaacac ttcataggtg accgcgatat tgggataggc
     3001 gaagggcacg tcgagcacac cgatcatcgg atacaccgag gcaatgccgc ccaccgacga
     3061 gatcgacgac tgcacgacac ccgacttgac ctgctggatg acctcgttgt ccttgcccag
     3121 ttggccgtcc gggaagatct ctaccttgac ggcgccgttg gtgccggact cgaccagcga
     3181 cttgaagacg atggccatcg cgccggtgcc gttgtcgaac gggtcgttct tgttcaggtg
     3241 ggccagcttg atcgtctttt gggccagggc cgcggttggc atcatgccga ccgaaacggc
     3301 cagcagggcc gaaattgcaa ttgacttgaa taggcgcata aagatctcca tcgggactag
     3361 ggttgaggat tgagcgagaa caactgaaaa tttgatcaaa gacccatcag acgcggcatg
     3421 gtgagcgtca ggctcggcca gtaggccaca aggatcagca cggcgacctc ggcgatcacg
     3481 aacggccaca tggccttcga gatgtcctcg atctttgcgc cggtgatcga actcaagacg
     3541 aacaggctgg cccccacggg tggcgtcatc aagccgatgt tgagcgccag cacgatcatg
     3601 atgccggcct ggaccggatt catgccgatc gacaccgcca gcgcgcccag gatcgggccc
     3661 agcacgatga ggatggcatt gatgtccatg aacatgccca ggatcagcag cagcaccagg
     3721 accatcagga tgatcatcga ggggtccgaa gtcaacccca ggaacaaccc ggtgatcagc
     3781 tggggcagct tcatgtaggt catccaccag ctcaggatcg aagcagcggc gatgatgagg
     3841 aacaccacgc cggtgatacg agctgtgcgc accagcattc cgacgatatc ttgcagcttg
     3901 acactgcgaa agacgaatac gccgaggaca agggcgtaga acacggcgat cgccgcacct
     3961 tcggtgggtg tgacgaagcc gccgatgatg ccgccgagaa tgatcaccgg catgacgagt
     4021 gcggacagac tgttcttgaa cgccaggaga atcccaagca ggctctggcg ctgtccgccc
     4081 ttggggagat tgaggcgacg cccgaggccg gcgatcacgc tcatgcaaac gaggcccatc
     4141 accagtcccg gcaggatgcc cgccgcgaac aggctggcga tcgacacgcc catcatggag
     4201 ccgtagatca ccatcaggtt ggacggcgga atggtcgggc cgatgatcga gccggcaacc
     4261 accacgccac aggcaaacgg tttcttgtag ccgttctttt ccatcgccgg caccatcgtg
     4321 ctgccgaagg ccgccgcctc cgcgaccgcg gcgccggtga tgcccgcgaa cagcaccgag
     4381 gaaaccatgc acgactgggc catgccgccg cgcaagtggc ccaccagcgc gtcggcgaac
     4441 ttggccaggc ggtccgtgat gccggccttg ttcatcaact caccggcgaa gatgaacagg
     4501 ggcatggcca ggaaggtgaa caggtccagg ccggcgaagt agcgctgcgg cgccatcgcc
     4561 agcatctcgg caccgccgat ctggaggatg cccagaacgc cggcaatacc aagaacgtag
     4621 ccgatcggca caccgaccgc cagaaggacg aagaaagttg caagtaccca gagcatcatt
     4681 cgttctccgc gaaatcggcg ggcaggccgg ctaccgtgaa atcgcgtatt cccatcaaca
     4741 cgagttgaag gcagaccagc gccgaggaag cgggtaccga ggcaaagggg atcgccatgg
     4801 tcatatcgaa aatcgtcgcg tactggctgg cgccttgctg cgtcatgccg ataccgaagt
     4861 agaagagaaa cgcgaagaac gcgaaggcca gcaagtcgat tgccacctcg aaccagcgcc
     4921 ggatcctgat gggaagccgg tgcagcaacg cagtcaccgc cacgtgctcg cgtcgcgcca
     4981 ccccgcagga caccgccagg agagccgtcc agatcatcag gtagcgcgcc agttcctcgg
     5041 cccaggtgac cggcagttcg aacacataac ggccgaagac gccgatccag atgtcgatca
     5101 ccaggatcgc gagcagtccg gcacacagca actcgatcca gcggttgatt ttgccgctga
     5161 gcgccgcgct cctgcctccc cagcgctgca tcggcggaag ttcagggctt atttccatgt
     5221 gtcgtctgcc aacctagtga tcagtggcgg atgtcgccga tcgacgcatg cagatcctcg
     5281 atcgcctcga gccgtttgcg gcttttcctg cccagttccc tggcgactgc cgcgcacaga
     5341 taattgacga cgctgatggc cgccccatag gaatcgaaga tcgaggcgcc accggtcagg
     5401 cagtggacga cgacatcgcc ggcacgcgac agcggcggca tgccggggct ggcgatgaaa
     5461 accaatcgtg cgccggcctg gcgcgcggca tcgacgatcg tctgcagcaa ggtggcgcgc
     5521 cggcggaaat caacgaccag cagcacatcg ccccggcgca gcgaggccag gtcctcggcc
     5581 agggtcatcg ccgcagtggg cagcgaaacg acatcctccc tgacctgagc caggaggtaa
     5641 gcggcatagg ctgccacgaa atgaccgttg cgcatgccga cgacatgcac gcgtggcgcc
     5701 ttggctgttt gcgcgactgc ctgctcgaga acggcactgt ccgactgcct gaagctttcc
     5761 gacaggtttt tcagatcggc ggcgagatgc cgcgacaacg cgtcggacct gcgttccttg
     5821 gcatccaccc cggcaagcgc ataaagcggc gaggccagat gcgactcggc acgagcccgc
     5881 cggcgtgcat gctcgaaact ctcgtagccg attcgccgga agaatctcgc ggcggtcgcc
     5941 tttgagacgg aggcatcagc cgccaattca gtggccgaat agctggcaag attgccgagg
     6001 cgggaaagaa ccgtgtcggc aagcttgcgc tcgcccggcg gcagactctc gtatgccgcc
     6061 atgatcgatt cctgaattgt cggctcgagt tgttgcatgc ggcgctgtgg cctcgctcgt
     6121 aacgttcgac ttgcagcagc acggcccgcc gggcgatcca ctccgcgggc atgaaactgc
     6181 agtttcttgt ttatatttcc ataaaacata tgtttcgtca accgttcgag ctgggaatca
     6241 actcatcggc ggcgatttgc gctacatcaa gcacgctcta ggaggaaccc ggaaggcgtc
     6301 cggcgagaat gcggtggaca atccccgttg ccgcccgcgg catcgttatt gcttgcccgt
     6361 cgtcgtccac accacccgga gttcacatga aaaaacgagt gttcctgcaa ctggccgccg
     6421 cccttgggct ggcctgcgga ttcggcgccg tccaggcgca gaccacgccc atcaagttcc
     6481 agctggactg gcgcttcgaa ggaccggccg cacttttcct gaccccggcc gccaggggct
     6541 acttcaagga cgcgaagctc gatgtaacga tcgacgcggg caccggttcc ggcgtggccg
     6601 tcacccgggt ggcgtccggc acctatgaca tgggctttgc cgacatggcg gccctgatgg
     6661 aattccacgc caacaacccc gacgcaccca acaagccggt ggcggtgatg gtggtctaca
     6721 acaacacgcc ggcatccgtc atggcgctga agaagtccgg catcaggacg ccggccgacc
     6781 tgaacggcaa gaagctgggc gcgcccgtgt tcgacgccgg gcgccgcgca ttccccatct
     6841 tccagaaagc caatggcgtc ggcagcgtcg cctggacggc gatggacccg ccgctgcgcg
     6901 aaaccatgct ggtgcgcggc gatgtggatg cgatcaccgg cttcaccttc acgtcgctgc
     6961 tgaacctgga agcgcgcggc gtcaaggccg aggacgtggt cgtcatgccc tatgcgtccc
     7021 atggcgtcaa gctctatggc aatgtggtca tcgccagccc gaagatcatc aaggagaacc
     7081 cggcggcggt gaaggcgttc ctggcggcgc tgaccaaggg caccaaagag gtgctgagca
     7141 ggccggaagc cgccgtcgcg gacgtcaagg cacgtgacgc gatcatcaat tccggcatgg
     7201 aaatccgccg gctcaaactt gccatcgaca cggtcatcaa cagctccgac gcgcgggccg
     7261 aaggtttcgg gcagatcaat ccggcccggc tcacgctgat ggcctcgcag gtgtcggacg
     7321 ctttcaacac caagacgcgc gtcaacccgg atgcggtctg gaacggctcg ttcctgccgc
     7381 ccaaagccga cctgaacgcc gtcctgcccg cccccaggaa atgacggaag gcccgttcgt
     7441 cgatttccag gacgtctggc tctcctacaa cgaggagctg ctgcggcaag ggcagtacgc
     7501 ggtcgaggcg atcgacctga cggtcggcag tggcgagttc atcgcaatcg tcgggccgtc
     7561 gggctgcggc aagtcgacct tcatgaagct ggccaccggg ctgaagatgc cctccaaggg
     7621 gcggattctg gtcgaagggc aaccggtcac gggccccctg aagatctcgg gcatggcatt
     7681 ccaggcgcct tcgctgctgc cctggcgcac cacggtggac aacgtgctgc tgccgctcga
     7741 aatcgtggag ccctaccgca gcaacttcaa gtccaggcgc aaggagtacg agcagcgggc
     7801 gcggctgctg ctgcagaagg tcggcctggg cggatacgaa gaccagtatc cgtggcagct
     7861 ctccgggggc atgcagcagc gcgcgagcat ctgccgggcg ctcatccacg agccgaagat
     7921 gttgctgctg gacgagccgt tcggcgcgct ggatgccttc acgcgcgagg aattgtggtg
     7981 catcctgcgt gacctctgga ccgagcagcg attcaacgtc atcctcgtca cgcacgacct
     8041 gcgcgagtcg gtgttcctgg ccgacaccgt ttacgtgatg agccaaaggc ccgggcgttt
     8101 catggtgagg aaggaagtcg acctgccgcg gccgcgcgac ctcgagatca cctacacgcg
     8161 ccagttcatc gacctggtgc acgagctgcg agggcacatc ggggctgtcc gcgagcaggc
     8221 ggccgcggcg gcggcggtgt catgaagacc acgcacctgg agcgctggtc gccgtggatc
     8281 ctgctggtgg cggtaatcgg cctttggcag atcgtgtgca gcgtcttcaa cgtgtcggag
     8341 ttcatctttc cgagcccggc ccggatctgg cagcagctgg tggaattcag gggcgccatc
     8401 gcggtccatg cttggcgcac gctctgggtc accgtggcgg gcttcgggct ggcgatcgtc
     8461 gtcggcgtgc tgctgggctt cctgatcggc agctcgcgca tcgcgtacgc cgcggtgtat
     8521 ccgctgatga ccgccttcaa cgcgctgccc aaggccgctt tcgtgccgat cctcgtggtg
     8581 tggttcggca tcggagccgg tcctgcgatc ctcaccgcct tcctgatctc gttcttcccc
     8641 atcatggtga acatcgccac ggggctggcg acgctggagc cggaactgga ggacgtgttg
     8701 cgggtgctgg gcgcgcggcg ctgggacgtg ctggtcaaag tggggctgcc gcgttcgatg
     8761 ccctatttct acgggtcgct gaaagtggcg atcacgctgg cgttcgtcgg caccacggtc
     8821 tcggaaatga cggccgccaa cgaagggatc ggctacctgc tgatctcggc cggctcgtcc
     8881 atgcagatgg gcctggcatt cgccggcctg gtggtggtcg gcgcgatggc gatgctgatg
     8941 tacgaactgt tcagctggct cgaaacgcac accaccgcgt gggcgcatcg gggttcgcag
     9001 gcccgttagc ccctgctaaa gccgtccctg ataggggtgg ccaaagggaa ggcgccccag
     9061 gtagcggccc cggccgcttt cggcgcgaag gtcgccatcg gcccagagat gagcgccgcg
     9121 gctgaacgtg tgcacgacca caccccgcac ctccatccct tcccacaccg agaaatcggt
     9181 gttctgccgc tgggtagcgg ccgacagcgt cctggtccct gccggatcga gaacgatcac
     9241 atcggcgtcc gcgccgattt cgagcccgcc cttgcgcggc caaaggccga aggtccgcgc
     9301 cgcgttggtc gatgtcagcg ccacgaactg ttccggcgaa atacggccgc cgttgacgcc
     9361 gaggtgccag agcacatgca agcggtcttc gatccctccg cagccattgg gtatccgggt
     9421 gaaatcgtcc tggccaaggc gcttctgggc cttggtgaaa cagcagtgat cggtgccggt
     9481 ggtgctgagc gcgccctccc gcaaggcctg ccaaaggact tcctggtgct cgcgcggccg
     9541 gtaaggcggg ctcatcacgt ggccggcggc gaaatcgaag tcggcatgcc ggtacaccga
     9601 gtcgtcgatg gcgaggaatc cggggagcgt ctcgccgatc accttcacgc cgcggccgcg
     9661 agcctgtgca atggcccgcg cggcttccgc cgccgataca tgcacgacgt acagcggcac
     9721 atcgatgatt tccgccatgg tgatggcgcg gcctgtcgct tcgccttcgc attgcggcgg
     9781 gcgcgagagc acatgaccca ggggcgaagt gacgccttcg gcgagcatct tcgcctgcag
     9841 gtaagcaatg agctcgccgt tctcggcgtg gacctgaggc agggctccca attcccggca
     9901 gcgccggaaa ccggcaatga gctcgtcgtc cggcagcatc aatccgcctt tgtaggccag
     9961 gaagaacttg aaactggtga tgccatggtc gcgcacgagg gcgcccatct cctcggaaaa
    10021 gcgcgggccc caccatgaca cggtcatgtg aaatccatag tcgatggccg ccttggcggc
    10081 ccggcttttc cagatggcca acgcctccag cggcgactgg ccgcgctcgg gcccgacgaa
    10141 gtccaggaag gaagtggtac cgccggcagc ggcggccgcc gtgccggtgt agaaatcgtc
    10201 ggcgacaacc gtccccatca tcggcagttg caaatgggtg tgcggatcga tgccgcccgg
    10261 catcacgaag catccgccgg cctcgatcac ttcggtgccg gcctgaagct ccaggcccgt
    10321 gccgatcgcg gcgatcaggc cgtcacggca cagcaggtcc gctcgccggc gcccgtcggc
    10381 cgtgaccagc gtgccgcccc gaatcagtgt gtcggtcatg gagttggtgt tgcggcaagg
    10441 agcaaccccg tgctgatgaa gttgttcgcc tggggactgg cgacggctgt ccggatcagg
    10501 tccatcatgc gcagccggcg cagatcgacc gcggcagtgg cctggccctc gaccgagccc
    10561 agcgggtcgt cgaggtcgag gcgaatgtcg aaaccttggc cggcgtacct cagcttgctg
    10621 ccgggggttg tttccacccg cacttcctgc ccttcgaagc ggccgagcat ggtcagggtg
    10681 tccccgtcca gtgcaaaagc gaagccgtcg cgatccggcg tttctttcca ggcggcggcg
    10741 ctgccgaaga aacgtgcctt ggccagatag ggcccaccgt cctccgggca gaacacatcg
    10801 caattgccgc actcgttgca ggcgtccgcg aagttgccga gctgccggtg ccttgcaaag
    10861 accagagcgc cggcaggttc gctgcgccat ccgccgggcg tcggcaccag tcgttcgacc
    10921 gggtaacttc ccggcggaat gacgaacggg aagttggcgt cgttgggaca gacgggcagg
    10981 catttgttgc aggcaatggt gcagtcgaag agcgccagcc gacttgcgat cttgcgtggt
    11041 gccgcagcgt gttcggcacg gctgtagcgg gggtcttcca gcagcctctc gacgtaggtg
    11101 ttcgtattgc gcagcacaca cgccgatacc caggccggga aatccgcgtc cgcggccgct
    11161 gccagatccc ctttgccctc cagcgccgac cggcaggcct cggcccgttc ccctggcagg
    11221 cccagatcct tcaaggctgc ttgcccatga cccaacgccc tgacgatgta ttcatcgatg
    11281 ccgcgagccc cgaccgcatc catgcgctcc tccagcgccg ccaggtactt gatggcgcgg
    11341 ccatagccgc ccgctttcag caggtccgag cagacagtga ccggcttcag gccgagcgcc
    11401 accgcgtcgg cgaaattgcc gtcgtcgatt cccgctgaaa acgaaaccgg gaagcggtcg
    11461 ccgaagaccg accggaaacg ctggaccaga accatggcca gcacgtgaag cggcaacccg
    11521 gacatgtaca tctgcgattc ggttgccgga aagaacgcct tgtggttctt gaccagcagc
    11581 gtgttgctga acttcacgcc gaagccgcgg ccaagccgcg ccgccagatc tcccagccgg
    11641 ttcactattg ccgtgacctg ttcccacttc gggtcgtttt caaaggccgc atcgggaacc
    11701 atgagctccg tatagcccag gcggccatgc aggatttcca gaagcgattc gcgacccagc
    11761 agcgtcggat tgagcttgac cacgacgttc aagtccatct cgcgcagcag gaaagcggca
    11821 atggcctcaa tttcgcccgg cggacagccg tggaaagtgg acagggtgag cgtgtcggaa
    11881 atgcgggtgc ggaacggcag gtcgcgaaag cgcgccagcg ccggcggaat ctgcgcgcgc
    11941 aggcgctcga cgaccgcgcc ggcgtccatc aacccggcca gaaagccgcg caccggcggc
    12001 gagccgatgc cggcaaggtc gtagccgacg ctcatgtcga agacggtatc ggtgcaaccg
    12061 gcagccatgc ccgacgcccg cagcatctcg atgagcatgg atgccttgac gtactcctcc
    12121 agcgattggg ccagcttgag ttcctgggac cattcgacgt tgaatcccac cgtctgcatg
    12181 tcgatgcagg gtctgggaat gtgaaggtca tccttgatct ggacggtctt caactccatg
    12241 aaccggccgc cgcacagcca ggccaggacg atgttctgcg ccatctgggt gtggggtccg
    12301 gcggctggtc cgaaaggcgt cgaagcccgg cctccgtgca gattcaagga aaggtctcgg
    12361 gtgttgctgc cgcggtaaaa aaagcgttcc ggcagatcga acgccgcctt cttttgttcc
    12421 agctccgaaa acaagcgggt cgccaggttc gcaaaaggaa tcgggatcaa ttcggccatt
    12481 gggtctggtc aagtgtgttt gcgaagttgc tgggcaatca ctgtgggctc tcggataatc
    12541 gcacttaatc agttggggac agagctgaag atctgtcccg ctcttaatca gttggggaca
    12601 gagcaaaatt gctgaagcaa ttcttgatct gtcccgcaag gtaaaaatga atctctatca
    12661 attctccctc aatgggacac ccgtcgaggt gcatgcccct cccgaccgct tgctgctgga
    12721 ggtgctgcgg gagaatttca agatagcgtc ggtcaagaac gggtgcgcac cgcagcaggc
    12781 atgcggcgcc tgcttgatcc tgatcaacgg cgtgccaaaa aactcctgcg ccctggacat
    12841 ggaaaagatg cagggcaagg aggtcctcac gctggaaggt gtcggcgagg atgaacgcca
    12901 gctgttcgcg cgggcgttcc aggcggcggc cggcctgcaa tgcgggtttt gcacacccgg
    12961 tttggtcttg cgcatcaaat ggctggccga tcgggggcga gcgctgtcgc gtcccgagat
    13021 cgccaaagcc ttgaccggcc atctctgccg gtgtacgggc tacgtgaaga tcatcgacgc
    13081 tgtcgagttg attctgaagg ccaagcgcgg cgggcagctg cctgagctgt tggagggcgg
    13141 gcgtgtcggc cagtcgctgc ctcgctatca aggactcgaa ttggcgctgg ggtcccggcc
    13201 tttcgtggcc gatatcgagg ttcccggatt gctgcacggc gccgtggtgc tttcgcccca
    13261 tgcacatgcg cgcgtcctcg gtatcgatac cggcaaggcc gctgcgctgt ccggcgtggt
    13321 gaaggtcatc acggcaaagg atgtcccggg cgaccgttgg gtgggccaga tctgcaagga
    13381 ctggccggtt ctggtggccg agggtgagga ggtgcgctat gtcggtgatg ttctcgcggt
    13441 cgtcgccgcc cagaccccgc acatcgcgcg tgcggcggct ggtcttgtag ccgtcgctta
    13501 cgaaccgctg ccgcccctgc tggacccggc cgaggcgatc aagccctcgg cgccgcaggt
    13561 caatcccagg cacgacaacg tgctgtcgcg caccagcatc cagcgcggcg cggtggatgc
    13621 ggcgctggtg tcgtccgccc acgtggtgag cggcacctgg cgcacccagc gcatcgagca
    13681 cctgtttctc gaaccggaag cggccttggc cgtgcctctt caagacggaa ggctgcatct
    13741 gttgacccag gggcaaggcg tgttcgacga ccgccgccag gtggcgtcgg tgctgggcga
    13801 atccgagaac aaggtgtttg tcgaactcgt gcccaatggc ggcgccttcg gcgggaaaga
    13861 ggacatgacg atccaggccc agaccgcgct cgcggcacgc ctgaccggca ggcccgtgcg
    13921 gatcgagctg aaccgcgagg aatccatccg catgcatccc aagcggcatc ccatcaccat
    13981 gacctatacc gtgggctgcg atgcgcaggg tcgcctgacg gccgcccgta tctatctgct
    14041 cggcgattcg ggcgcctacg cctctgtcgg cggcaaggtt ctcgagcggg ccgccggcca
    14101 tgcctgcggg ccttaccgga ttccggctat cgacatcgaa gccgtggccg cttacaccaa
    14161 caatccgccg tgtggtgcca tgcgcggctt cggcgtcaac cagacgagtt ttgccatcga
    14221 aggctgcctg gacctgctgg cgaagaaagt gggcatcgac ggttgggaaa tgcggtggcg
    14281 caatgcggtg cgcgttggcg acgttttcag cagcggtcag gtactcgaca agtccatcgg
    14341 tctcgaacgc accttgttgg ctgtgaaaga cacctattac gccgcgcgca gcagcggacg
    14401 ggcggtgggc ctcgcttgtg gcgtcaagaa ttccggcatc ggcaacggcg ccatcgaaaa
    14461 aggcaagtgc cggcttcagg tggaggccga cgccagcgtc agcctgtatt ccgggttcac
    14521 cgagatggga cagggattgt tcaccgtcct gatccagttc gccgtcgaag tcaccggcct
    14581 cgcgccttcc gtatttcgac ccaaggtgac cagccggttc gaactgggaa gcggccagac
    14641 cacggggtcg cgcgcgacgc tgctggccgg gcgcgccacc attgacgccg ccggcaaact
    14701 cagcgtcgat ctgaaggcgg ggcatacgct caccgatctg gccgggcgga tctacgaagg
    14761 cgagacgacc atcgacgaca ccacggcgcc cggccagacg aagaacggaa tcatcaagac
    14821 ccatacgtcg ttcggctggt cgtcccaggt cgtggttctc gacgagcggg gcgcggtcga
    14881 gctggtgcat gtggcccagg acgtcggccg cgccatcaat ccgcagcaat gcgaggcgca
    14941 gatcgagggc gcggtggcga tggggctggg ttacgcattg accgaagagc ttccctgcaa
    15001 ggacggcatg ccggtaacct tccacccgcg tgaaatcggt gtcttgcggg cgccgcacat
    15061 gccgagagtc gaagtcacct tgatcgaaga acatgagccg gaaggtccgt tcggtgccaa
    15121 gggcatcggc gaactcggcc tggtgcccac ggccggtgcg gtggccgcgg cactcgaggc
    15181 gttcgacggc gtgcggcgca tgaccctgcc gatgaaggac tccgcggcag cgcgcgccat
    15241 cgatgtcggc ctcatcaagg acggcgacag ggatcaatgg cactgacgcc accattacgg
    15301 atcggccccg accgccttgg gcgcagcgtg gctgtggccg acgggctcgt cgttgcgaca
    15361 gcgccgaccg atgcgatacg gctgccttgc gcgcaagggg agatcgccgc cggagccgtc
    15421 tgcgcgcaca cccatctcta cagcggcctg gtccgttacg gaatgccgcc tgcgacaccg
    15481 gcgccggaga cattcctgaa aatcctggaa cgggtatggt ggcgactcga ccgcgtcctt
    15541 gacgccgaga cattgcgggc cgcggcgatg gactatgtgg cgcgagcact gcttgcggga
    15601 acgacgacgc tggttgacca ccacgaatcg cccaatctga tcgaaggatc gctggaaatc
    15661 ctggccgagg tctgcgggca actgggcgtg cgcgcgctcc tgtgctatgg cgcgaccgaa
    15721 cggaacttcg gcgcggcgga agcccgtcgc ggcctggacg aatgccggcg ggtgcctgcc
    15781 tcaccgctgc tgcgtggcct ggtcggactg cacgcgagct tcaccgtgtc ggacgacacg
    15841 atccgggccg ccgctcgcct ggctgaagat ctgggcacgg tcgtccacgt ccacgtggca
    15901 gaggacgcgg ccgatgtcgc cgatgcgctc gcgcgcggct atcccgggcc tttggagcgc
    15961 ctggaccgcc tcggtgccct gccgcgcggt tcgatcctgg ctcatggcgt gcatctggat
    16021 ggcgaacaag cgcgccattg tgatgaattg ggttgctggc tggtgcacaa cccgaggtcc
    16081 aacgagggca accgggtcgg ctacgcgctg aacctgggct gcagcaatcg cgtggccctg
    16141 ggctgcgacg gctgggacgc ggacatgacg acggaacagg aggcgctctc ccgtcttgcc
    16201 ggaattcatg gcgacgttaa ggcgcagggc cggctggcga atgggcacgt cctgatcgcg
    16261 gaacggtttg ccagccatcc cgagcctttg caacccggtg cgctcgggga tgtggtggtc
    16321 aggcaggacg gagccgtgag gcatgtggtc gtgggcgggc gtgtggtcgt cgccgacggc
    16381 gttctggcca cgggcgactt cgacagcatc accgccggcg ctcgcaagca ggccggccgc
    16441 ctttggcagc gaatggcgaa tacctgactt gtatcggcct gctacctagt gtagtgtcca
    16501 gcaaataact ggatgtattg cgcggcttct gatatgctgg cgcatgccca gaacaagccc
    16561 tgctttgaac ctgccagatg ctgatggcaa gcaactggag cagtgggctg cggcctacgg
    16621 gacgccgcag caagtggcgc tgcgatgcag gattgttttg gctgctgctg cgggcgtgac
    16681 aaatctggag attgcggcgg cgttgcaggt cgatgtaaag acagttgcgc tgtggcgcag
    16741 gcggtttgca caggtcggtc cagaaggatt gtgggagatt gccgctgggc gtggacgcaa
    16801 ggccactcat tcgccagaca agatcaaggc gattgtccaa gccacactgt cgacaaagcc
    16861 tcaaggaatg acccaatgga gttgtcggca aatggcgcaa gccgaaggtg tgagcaaatc
    16921 cacggtcagc aatatctggc gcagtcacaa cttgaagccg caccgtatca agagcttcaa
    16981 gctctcgcgc gatgcgctgt tcatggaaaa gctcaccgat gtggtggggc tgtatttgaa
    17041 tcctcccgat caggctctgg tgctatgcgt ggatgagaag acccagatcc aggcgctgga
    17101 ccgcacccaa cctggattac ccatgaagaa ggggcgcctg ggcacgatga cccacgatta
    17161 caagcgcaac ggcaccacgt gcctgttcgc cgcgctggaa gtgctgcaag gcaaagtcat
    17221 cggccagtgt tacagccggc atcgtcatca ggagttcctg aagttcctgc ggcaactgga
    17281 tgcggagttt cccggcgaca ttgccttgca cttggtgatg gacaactacg gcacgcacaa
    17341 gcaccccagc gtgcagagct ggctaaagcg ccatccgcga tttgttccgc actttgtccc
    17401 caccagttcc agttggctta acttggtcga acgctggttc ggtgaactca ccaccaaagc
    17461 cgtacgacgc ggctccttca gcagcgtcgc cgatctacag ttggccatcg ctgaatttct
    17521 cgacgcctgg aatgaaaacc ccaagccttt cgtctggacg gctacggtgg attccattca
    17581 ggccaagctg tctcggtgcc gtcagacctt ggagcagatc aagcccggct gcaccgcacc
    17641 acggacccga aagagaatca aatctccagt tatttccggg acaccacact agcatgtgga
    17701 atcgtcacgc tcgcgctgcc gtccggcttt ccccgcccga cctgatcgga cggtcgtgtg
    17761 ccggaggtac ggagcca
//
LOCUS       DASWIY010000412        17972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1032470, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000412 DASWIY010000000
VERSION     DASWIY010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 17972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17972
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032470"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VGA59_14465"
     CDS             complement(<1..1037)
                     /locus_tag="VGA59_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9720924.1"
                     /translation="MTRWALHEAGDCMTGWGFAAWLMLGGSTVLLFIGMPVALTFMTV
                     NIIGAWLYMGGEPGLAQLARSSVSSITAFALTPIPLFVLMGEVLFHTGLALKVIEGVE
                     RLIRRLPGRLAVVAVVAGTVFSAISGSTIATTAMLGSLMLPVMLARGYHPTLATGPIM
                     AIGAVDMLIPPSALTVLLGSLSGISISKLLIGGVLPGAILSVAFVLWIVVRVRISPHL
                     APPDESEVHYTGWERWKPFFGYVLPTLSIFGIVVGALANGWATPTECAALGAFATMVL
                     AVAYRALTFDALLRALKGTAGISGMILFIILGATTFAQILSFSGASNGLVQFITGQGL
                     STPMIVAGMMML"
     gene            complement(1015..1545)
                     /locus_tag="VGA59_14470"
     CDS             complement(1015..1545)
                     /locus_tag="VGA59_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9720925.1"
                     /translation="MIDRLSGAYGRLLAGLALVGCAILMAMMIIIVADVALRNIAVPG
                     LPRGLAWSNEISELMLYLITMFVAPWLLRQGQHIRIDILLQAIPKRLAWYSEWVSDVL
                     GLACCCVIAWYGGQAAWSSYASGAVNIKTLVTPEWWGLAPLPAVFILLAIEMIFRMIR
                     LQRAERGPRHDAVGAA"
     gene            complement(1542..2513)
                     /gene="dctP"
                     /locus_tag="VGA59_14475"
     CDS             complement(1542..2513)
                     /gene="dctP"
                     /locus_tag="VGA59_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9720926.1"
                     /translation="MNLHALRTAAALLFAAGFAAAAGAQETTLRLVSAFAENGIYVQR
                     LQPWIQKFNAEGKGVLQINFIGGPKAIPPFEAGNAVKTGVVDMAMSTGAFYTNVMPEA
                     DFLKLTQIPVAQQRKNGAFDAINKVWNEKGNMQYLARMVENQPFHIYTNKKVDKPDLT
                     GLKIRITPVYRDFFQALNASVITTPPGEVYTALERGVVDGYGWPIGGIFDLNWHEKTK
                     FRIDPGFYDAEVSLIMNLPAYKKLSEPQRAYLQKQLLALESGNTFWARYTTDEIARQE
                     KAGIQTIRFDAATSKAFRDKAYEVGWASAVKQSPEIAARFKPLFVPK"
     gene            2748..4238
                     /locus_tag="VGA59_14480"
     CDS             2748..4238
                     /locus_tag="VGA59_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9720927.1"
                     /translation="MDTTSVWRATAPQSAFAPLQGDTSTDILIVGGGITGVTLALLLA
                     RQGRSVVLFEAGTIGGGSTGNSTGNLYETLSQGLHGITRAWDEDVARQVLAQRRAAID
                     FIESEARGADCGFRRCALRLYATTEQHQFSIGQENAALAKAGANVRLERAVPAPLPTP
                     LGDVLALPDQAQFQPQAYVTHLAVLAAQAGAGVHEHSRMIGLEQKGKRAATANGTVKA
                     QEIVLATHTPAGFHMVQAEMPVHREYGIARELGRTDPGPGIFWCRGSEFLSVRTFETQ
                     ARRFLICVGQSNKTGSHNAKASLMALEMTVEKQFGSGTTAYCWSAQNYQAADRLPYIG
                     RDLSGCFIATGFSTDGLVWGTVSARIIAEQLMGQSPEFGTLCKPTRWPLKGGRTMMEE
                     VGTMAKALVKDYLTHRQEQALSSLAAGDSAIIEADGESCAAWRAPDGELFAVSPVCTH
                     LGCKVHWNSVETSWDCPCHGSRFRPDGSVIEGPAIRALRRKHVNVG"
     gene            complement(4250..4492)
                     /locus_tag="VGA59_14485"
     CDS             complement(4250..4492)
                     /locus_tag="VGA59_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04438 family Trp-rich protein"
                     /protein_id="HEX9720928.1"
                     /translation="MYFLGIGIVLLALKYLEFGPVAAWDWLVVLSPFALAVAWWFWAD
                     WSGYTKKKAMEKENARKQARIDKSREAMGIKPNRRR"
     gene            4619..4924
                     /locus_tag="VGA59_14490"
     CDS             4619..4924
                     /locus_tag="VGA59_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9720929.1"
                     /translation="MKRALFALIAATASVSAMADLALATSKNCMACHAMDKKLVGPAY
                     KDVAAKYAGQKDAVDKLAVKIMKGGSGVWGPVPMPANTQVNEADAKKLAAWVLSLKK"
     gene            complement(5054..7039)
                     /gene="acs"
                     /locus_tag="VGA59_14495"
     CDS             complement(5054..7039)
                     /gene="acs"
                     /locus_tag="VGA59_14495"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX9720930.1"
                     /translation="MSAIESVLVENRVFPPSEATVKAARISGMEAYNALCDEASRDFE
                     GFWARLARENVVWSKPFTRTLDESNAPFYKWFDDGELNASANCLDKHMGTPVENKTAI
                     IWEADDSSVVEVTYKELLARVCQFANALKAHGIRKGDRVVVYMPMCIEAVVAMQACAR
                     IGATHSVVFGGFSAKALNERIIDAGAVAVVTANFQMRGGKEMPLKAIVDEGIDIGGCH
                     TLKSVLVFMRTPTPCNMVAGRDQTFTEVLQGQSTECRPEPVGAEHPLFVLYTSGSTGK
                     PKGVQHSTGGFLLWAKLTMDWVFDIKPSDVFWCTADIGWVTGHTYVAYGPLAAGCTEV
                     MFEGIPTYPNAGRFWQMIQRHKVSVFYTAPTAIRSLIKAAEADEKIHPARFDLSSLRI
                     LGTVGEPINPEAWMWYYKNVGGERCPVVDTWWQTETGGHMISPLPGATPLVPGSCTLA
                     LPGIMAAIVDDLGKDLPHGDGGLLVIKRPWPAMIRTIWGDPERYKANYYPPELGGKLY
                     LAGDAAARSVDRGYFRIGGRVDDVLNVSGHRLGTMEIESALVSKTDLVAEAAVVGRPD
                     DLTGEAVCAFVVLKRSRPVGEEAKQIANELRNWVAKEIGPIAKPKDIRFGDNLPKTRS
                     GKIMRRLLRSIAKGEAITQDVSTLENPAILEQLAEKL"
     gene            7277..7429
                     /locus_tag="VGA59_14500"
                     /pseudo
     CDS             7277..7429
                     /locus_tag="VGA59_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
     gene            complement(7633..9018)
                     /gene="fumC"
                     /locus_tag="VGA59_14505"
     CDS             complement(7633..9018)
                     /gene="fumC"
                     /locus_tag="VGA59_14505"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX9720931.1"
                     /translation="MNIRIERDTFGPIEVPAERLWGAQTQRSLQNFDISGERQPREIV
                     RALVQIKRSSAVVNHLLGLMDDGKASAIIAAADEVLAGRHDDEFPLVVWQTGSGTQTN
                     MNVNEVLANRASELLGGPRGEGRLIHPNDDVNMSQSSNDVFPTAMNVAAVDAIRNRLM
                     PSLMKLKATLGAKAGAFHDIVKIGRTHLQDATPLTLGQEFSGYAAQLEQADRHLHGSL
                     PHLCELALGGTAVGTGLNAPEGYAEKVAAELARLTGLPFVTAPNKFEAMAAADALVHA
                     HGALKTLAASLMKIANDVRWLASGPRSGIGEINIPENEPGSSIMPGKVNPTQSEALTM
                     LCCQVFGNDVAINFGGASGNFELNVFRPMIAHNFLQSVRLLADGMASFNDHCAAGIEP
                     NRERIAELVGRSLMLVTALNPHIGYDKAAQIAKKAHKEGTSLRDAAIATGFVTAEQFD
                     QWVRPEKMVGK"
     gene            9129..10673
                     /locus_tag="VGA59_14510"
     CDS             9129..10673
                     /locus_tag="VGA59_14510"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX9720932.1"
                     /translation="MTTTIIKQSDLTESIAAALQYISYYHPADFIAHLARAYEREQSP
                     AAKDAIAQILTNSKMSATGHRPMCQDTGIVNVFLKVGMDVRFEGFTGNLDDAINEGVR
                     RAYNHPDNTLRASVVADPQFERKNTKDNTPAVIFTELVPGNKLDVTVAAKGGGSENKS
                     KLAMLNPSDSVVDWVLKTVPTMGAGWCPPGILGIGIGGTAEKAMLMAKESLMDHVDMY
                     ELQQRGPKDKTEEMRLELYEKVNALGIGAQGLGGLTTVLDIKIKMYPTHAAGKPVAMI
                     PNCAATRHAHFVMDGSGPVYLDPPSLDLWPKVQWAPDYEKSRKVNLDALTREEVASWK
                     PGDTLLLNGKMLTGRDAAHQRIQDMLAKGEKLPVDFTNRVIYYVGPVDPIKGEAVGPA
                     GPTTATRMDKFTEMMLAQTGLIAMIGKSERGPVAIEAIKKHKSAYLMAVGGAAYLVSK
                     AIKTAKVVGFGDLGMEAIYEFDVVDMPVTVAVDAGGTSAHITGPAEWQKKIASGEFKS
                     IPVAAA"
     gene            complement(10802..12115)
                     /locus_tag="VGA59_14515"
     CDS             complement(10802..12115)
                     /locus_tag="VGA59_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9720933.1"
                     /translation="MPLFSRDALNPGVRKREVFGWAMYDFANSGYTTVVITAVFAAYF
                     VGGIAGKAEWATFAWTAALSVSYAIVMLTMPSIGAYADLRAAKKRLLALFTGGCVVST
                     AALALAGPGSVALAMLLIVVSNTFYSYGESLTAAFLPELARPDSLGKVSGWGWSFGYF
                     GGMMALGLCLGYVIWAQGQGIAAERFVPVTMLITAVLYGSAALVTFWLLGERARPNPA
                     ALRQGGLKASLQQLHRTFGQARRFEDFMWLLSCAALYQGGVAVAVTLAAIYAETVIGF
                     QQQETMVLIFVLNIAAAAGAFGWGYLQDRIGHKIALGSTLVGWVATCIIAAVTTTKGG
                     FWWAAAIAGLCMGSSQSAGRAMAGMFAPRNQLAEFYGLWTFAIRLASIIGPLSYGAIT
                     WATGGNQRLAIMSTAGLFIAGLLLLVPVNVRRGREAALEADAAAA"
     gene            complement(12233..12856)
                     /locus_tag="VGA59_14520"
     CDS             complement(12233..12856)
                     /locus_tag="VGA59_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HEX9720934.1"
                     /translation="MSRYNAPFEIHVHGEVPLRPDVSYAQLQDALKPLWTYAGAKSLA
                     DGAESSYEEEPGIKFDAPEHLLRMCWTVPGDEDFRQSLDEMCMSLNELSEQGAAIEVT
                     FYDAEFDEEEEAPEAESRDDFVMLFVGPTPAAIMQVQRDLLVEDVVNLMERHFDGSEL
                     GGVVAEIDKLFSQRFDALVNSLEIGKPPRGLGGGPGHGGGRRPRHLH"
     gene            13142..13234
                     /locus_tag="VGA59_14525"
     tRNA            13142..13234
                     /locus_tag="VGA59_14525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13176..13178,aa:Leu,seq:taa)
     gene            13350..13961
                     /locus_tag="VGA59_14530"
     CDS             13350..13961
                     /locus_tag="VGA59_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720935.1"
                     /translation="MKKFVLTAILAAILTGCGGGDSDAPAGPVAVPQRLVGEVIAYVP
                     HVLQHRLEAFTTAAALVRKHIPQARVGVTITPYAMIGRTNSMEYAKRAIALSDWVGTD
                     PYWFGGDNVKELNDWSHSFSAVAKQANPAVETWLIAQAFKMPEWDTATFNAFTKEQLA
                     AAEGYDHILFFGWQFVSEIDTSAAGMHFGAETKALFSKYLKAQ"
     gene            complement(14018..14091)
                     /locus_tag="VGA59_14535"
     tRNA            complement(14018..14091)
                     /locus_tag="VGA59_14535"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14057..14059),aa:Cys,seq:gca)
     gene            complement(14140..14215)
                     /locus_tag="VGA59_14540"
     tRNA            complement(14140..14215)
                     /locus_tag="VGA59_14540"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14180..14182),aa:Gly,seq:gcc)
     gene            complement(14269..16707)
                     /gene="lon"
                     /locus_tag="VGA59_14545"
     CDS             complement(14269..16707)
                     /gene="lon"
                     /locus_tag="VGA59_14545"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX9720936.1"
                     /translation="MSGHIPLPATAIDLPLLPLRDVVVFPHMVIPLFVGRPKSIKALE
                     AAMEAERRIMLVAQKAAAKDEPSVSDMFEVGCVSTILQMLKLPDGTVKVLVEGQQRAR
                     VNRIEDGEQHFSANVTPVESAEKDPKSSEIEALRRAVMQQFDQYVKLNKKIPPEILTS
                     ISSIDDPGRLADTIAAHLPLKLDNKQIVLDLADVKLRLENLFEQIEREVDILNVDKKI
                     RGRVKRQMEKNQRDFYLNEQVKAIQKELGEGEEGADIEEIEKKIKAAKMPKEAKKKAD
                     GELKKLKLMSPMSAEATVVRNYIEVLTALPWSKKTKIKHDLGHAEDVLNQDHHGLEKV
                     KDRILEYLAVQKRVDKLKAPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGMRDE
                     AEIRGHRRTYIGALPGKVLSSLSRVGTRNPLFLLDEIDKLGTDFRGDPSSALLEVLDP
                     EQNHTFGDHYVEVDFDLSDVMFVATSNSMNIPPALLDRMEVIRLSGYTEDEKTSIALK
                     YLLPKQMKNNGVKESELEVTEEAIRDIIRYYTREAGVRSLERDLSKICRKTVKGLQLK
                     KMTPQVVVTAENLNDFLGVRKFTYGRAEQQNQVGQVVGLAWTEVGGDLLTIEAALTPG
                     KGVITSTGSLGDVMKESVAAARTVVRSRSRRLGIPDEVFEKKDMHIHVPDGATPKDGP
                     SAGAAMTTAIVSALTGIPVRADVAMTGEITLRGEVTAIGGLKEKLLAALRGGIKTVLI
                     PQENAKDLQEIPDNVKSGLEIVPVKWIDKVLEVALERNPTPLPDEEPAAVPVAAPIAG
                     DGEQPAGVRVSVKH"
     gene            complement(16829..>17972)
                     /gene="clpX"
                     /locus_tag="VGA59_14550"
     CDS             complement(16829..>17972)
                     /gene="clpX"
                     /locus_tag="VGA59_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX9720937.1"
                     /translation="ELCNDIIREELEEKAEKTREKLPKPQEIKRVLDDYVIGQNRAKK
                     VLSVAVYNHYKRLQTKTSEQRAKDEVELAKSNILLIGPTGSGKTLLAQTLARMLDVPF
                     VMADATTLTEAGYVGEDVENIIQKLLQSCNYEVERAQRGIVYIDEIDKISRKSDNPSI
                     TRDVSGEGVQQALLKLIEGTMASVPPQGGRKHPNQDFLQIDTTNILFICGGAFSGLEK
                     VIEQRTEASGIGFGAAVKSKAQRSLSDAFREVEPEDLIKFGLIPELVGRMPVVATLSE
                     LSEDALVRILTEPKNALVKQYGKLLSMEGVDLEIRPNALKAIARKALARKTGARGLRS
                     ILEQSLIDTMFELPNTANVDKVVVDESTIDENKPPLLVYREAAKKA"
BASE COUNT         3132 a         5894 c         5750 g         3196 t
ORIGIN      
        1 agcatcatca tgccggcgac gatcatgggg gtggacaggc cctggccggt gatgaattgc
       61 accaggccgt tgctggcgcc ggaaaacgac aggatctgcg cgaacgtggt ggcgcccaga
      121 atgatgaaca ggatcatccc cgagatgccg gccgtgccct tgagcgccct gagcagcgcg
      181 tcgaaggtga gcgcgcggta cgccacagcg aggaccatgg tcgcgaaggc gcccagcgct
      241 gcgcactcgg tgggcgtggc ccagccgttc gccaacgctc cgaccacgat gccgaagatc
      301 gagagcgtgg gcagcacgta gccgaaaaac ggcttccagc gctcccagcc ggtgtagtgg
      361 acttcgctct cgtccggcgg cgccagatgc gggctgatac gcacccgcac cacgatccac
      421 agcacgaagg cgacggacag gatggcgccg ggcagcacgc cgccgatcag cagcttggaa
      481 atcgagatgc cggacagcga gccgagcagc accgtgaggg ccgagggcgg gatcagcatg
      541 tccaccgcgc cgatggccat gatggggccg gtggccagtg tggggtggta gccgcgcgcg
      601 agcatcaccg gcagcatcag cgaacccagc atcgcggtgg tggcgatggt ggaaccggag
      661 atggcggaaa acaccgtccc ggccaccacg gccaccacgg ccagccgccc gggcaggcgc
      721 cggatcaggc gttcaacgcc ttcgatcacc ttcagcgcca ggccggtgtg gaacaggact
      781 tctcccatca acacgaacag cgggatcggc gtgagcgcaa acgccgtgat cgaactgacg
      841 ctgctgcgtg ccagttgggc cagcccgggc tcgccgccca tgtagagcca ggcgccgatg
      901 atgttcacgg tcatgaaggt cagtgccacc ggcatgccga tgaacagcag taccgtggag
      961 ccgcccagca tcagccaggc cgcaaagccc cagccggtca tgcagtcccc cgcttcatgc
     1021 agcgcccacc gcgtcatggc gcggcccgcg ttcggcccgc tgcaggcgga tcatgcgaaa
     1081 aatcatttcg atcgcaagaa gaatgaacac agccggcagc ggcgccagcc cccaccactc
     1141 cggcgtcacc agggttttga tgttcacggc gccggaggca tagctggacc aggcggcctg
     1201 gccgccgtac caggcgatca cgcaacaaca ggcgaggccc aggacgtcgc tcacccattc
     1261 ggagtaccag gcaagccgct tcggaatggc ttgcaggagg atgtcgatcc ggatgtgctg
     1321 gccctggcgc agcagccagg gcgcgacgaa catcgtgatg aggtagagca tcagctccga
     1381 aatctcgttg ctccaggcca agccgcgcgg caggccggga acggcgatgt tgcgcagcgc
     1441 cacatcggcc acgatgatga tcatcatggc catgagaatg gcgcagccca ccagggccag
     1501 cccggccagc agacggccgt aggcgcccga gagccggtcg atcacttggg cacgaacagc
     1561 ggcttgaagc gggcggctat ttcggggctc tgcttgacgg cgctggccca acccacttca
     1621 taggccttgt cccgaaaagc cttggacgtg gcggcatcga acctgatggt ctggatgccg
     1681 gctttttcct ggcgcgcgat ttcgtcggtg gtgtagcggg cccagaacgt gttcccggat
     1741 tccagcgcca gcagctgctt ttgcagatag gcgcgttgcg gctcgctcaa tttcttgtag
     1801 gccggaaggt tcatgatgag cgaaacctcg gcatcataga agccggggtc gatgcggaac
     1861 ttggtttttt cgtgccagtt caggtcgaaa atgccgccga tgggccagcc atagccgtcc
     1921 accacgccgc gctccagcgc cgtgtacacc tcgccgggcg gcgtggtgat gacgctggcg
     1981 ttcagggcct ggaagaagtc gcgatacacc ggcgtgatgc ggatcttcaa gccggtgagg
     2041 tcgggcttgt cgaccttctt gttggtgtag atgtggaacg gctggttttc caccatgcgc
     2101 gccaggtact gcatgttgcc cttctcgttc cagaccttgt tgatggcgtc gaaggcgccg
     2161 ttcttgcgct gctgggccac cgggatctgg gtgagcttga ggaagtcggc ttccggcatg
     2221 acgttggtgt agaacgctcc ggtgctcatc gccatgtcca ccaccccggt cttcaccgcg
     2281 ttgcccgcct cgaagggtgg gatcgccttg ggaccaccga tgaagttgat ctgcaggacg
     2341 cccttgcctt cggcgttgaa cttctggatc cacggctgca ggcgctgcac atagatgccg
     2401 ttctcggcga aggcgctgac caggcgcagc gtcgtttcct gtgcgccggc cgcggcggca
     2461 aagccggcgg cgaacagcag cgcggctgcg gttctcaatg catgcaaatt catgatgcct
     2521 gtctccttat agtcttcggt tgaagttaaa cgcttgggct gacgccgtca attcgcacag
     2581 acgccaacaa tttctgcagc ccctgccggc catagtgccc agcgcgtaac ccggcgcacc
     2641 tgtggggacc cggtgctcga caacggctag ccggggttgc gcgcttgtcc tacatctggg
     2701 ccggcaacgg caaccctagc atggcgtttt cccagcggaa aaaagccatg gacactactt
     2761 cggtctggcg cgcgacggcg ccgcaatcgg ccttcgcgcc actgcaaggc gataccagca
     2821 ccgacatact gatcgtgggt ggcggcatca ccggcgtgac gctcgcactg ctgctggccc
     2881 ggcaaggccg gtcggtggtg ctgttcgaag ccgggacgat cggcggcggc agcaccggca
     2941 attcgaccgg caacctgtat gaaactctca gccagggcct gcacggcatc accagggcct
     3001 gggacgagga cgtggcgcgg caggtgctgg cccagcgccg cgccgccatc gacttcatcg
     3061 aaagcgaggc ccggggtgcc gactgcggat tccggcgctg cgccctgcgc ctgtatgcga
     3121 ccacggagca gcaccagttc tccatcggcc aggagaacgc ggcgctggcc aaggccggtg
     3181 cgaacgttcg cctggaacga gcggttccgg cgccgctgcc gaccccgctg ggtgacgtgc
     3241 tcgcgttgcc tgaccaggcc cagttccagc cacaggccta cgtgacgcac ctggcagtgc
     3301 tggcggcgca ggcgggcgcc ggcgtgcatg aacactcgcg catgatcgga ctggaacaga
     3361 agggcaaacg cgccgccacc gcgaacggca ccgtgaaggc ccaggagatc gtgctggcga
     3421 cccacacgcc cgcgggcttc cacatggtcc aggccgaaat gccggtgcac cgcgagtacg
     3481 ggatcgcccg cgagctgggt cgaaccgacc ccgggcccgg catcttctgg tgccgcggaa
     3541 gcgaattcct gtcggtgcgc acattcgaaa cgcaggcgcg gcgctttctc atatgtgtcg
     3601 ggcaatccaa caagacgggg tcccacaacg ccaaggccag cctgatggcg ctcgagatga
     3661 cggtggaaaa acagttcggc tccggcacca ccgcctattg ctggtcggca cagaactacc
     3721 aggcggccga tcgcctgccg tacatcggcc gcgacctgtc cggctgcttc atcgccaccg
     3781 gtttttcgac cgacggtctc gtgtggggaa ccgtgtccgc gcgaatcatc gcggagcaac
     3841 tcatggggca gtcgccggag ttcgggacct tgtgcaagcc cacgcgctgg ccgctcaagg
     3901 gcggcaggac catgatggaa gaagtgggca ccatggccaa ggcgctggtc aaggactacc
     3961 tgactcaccg gcaggaacag gcgctatcga gcctcgcggc cggcgacagc gccatcatcg
     4021 aggccgacgg cgagtcgtgc gcggcgtggc gcgctcccga cggcgagctg ttcgccgtat
     4081 cgccggtctg tacgcacctg ggctgcaagg tgcactggaa cagcgtggaa accagctggg
     4141 actgcccctg ccatggcagc cggttcaggc ccgatggatc ggtgatcgag ggacccgcga
     4201 tcagggccct caggcgcaaa cacgtgaatg tcggataaga cggtgcgggt cagcggcggc
     4261 ggttgggctt gatgcccatc gcttcgcggc tcttgtcgat gcgtgcctgc tttcgcgcgt
     4321 tctctttttc catcgccttt ttcttcgtgt agcccgacca gtccgcccag aaccaccacg
     4381 ccacggccag ggcgaacggc gagagcacga ccaaccagtc ccaagccgcg accggcccga
     4441 attcaaggta cttcaaggcc agcaggacga ttccgattcc caggaaatac atagcgttac
     4501 tccgacgaag cgctgctcgt tcccggtcga gggctttacc ccgcagagac atgagcgtca
     4561 accgcaatca ctacaatgac gttgaagcac tgtaacccaa ctgaagagga cgaaaccaat
     4621 gaaacgtgcc ctgtttgcac tgattgcggc aaccgccagc gtctccgcga tggcggacct
     4681 ggcgctggcc acgagcaaga attgcatggc ctgccatgcc atggacaaga agctggtcgg
     4741 accggcctac aaggacgtcg ccgccaaata cgccggccag aaagacgccg tggacaagct
     4801 ggccgtcaag atcatgaagg gcggctccgg tgtctggggc ccggtgccga tgccggccaa
     4861 tacccaggtg aacgaagccg atgccaagaa gctggcggca tgggtgctga gcctgaaaaa
     4921 ataaggacgc agacccagga acaagcccgc ctcccggcgg gctttgtttt gcctgttctg
     4981 gctgtcaccc cggacttgat ccggggtcca caacggcacc tgcgtggatt gcggatcaag
     5041 tccgcaatga cagttacagc ttctcggcca gctgctccag gatcgcgggg ttctcaaggg
     5101 tcgaaacgtc ctgggtgatg gcctcgccct tggcgatgga acgcagcagc cggcgcatga
     5161 tcttgccgct gcgggtcttg ggcaggttgt cgccgaaccg gatgtccttg ggcttggcga
     5221 tcggaccgat ctccttggcg acccagttgc gcagttcgtt ggcgatctgc ttggcctcct
     5281 cgcccaccgg gcgggaacgt ttgagcacca cgaaggcaca gacggcttcg ccggtcaggt
     5341 cgtccggacg gccgaccacc gctgcttcgg ccaccaggtc ggtcttggac acgagcgcgg
     5401 attcgatttc catcgtgccc aggcggtgac ccgagacatt gaggacatcg tcgacgcgtc
     5461 cgccgatgcg gaagtagccc cggtccacgc tgcgggccgc ggcgtcaccc gccaggtaga
     5521 gcttcccgcc cagttcgggc gggtaatagt tggccttgta gcgctccggg tcgccccaga
     5581 tcgtgcggat cattgccggc cagggccgct tgatcaccag cagcccgcca tccccgtggg
     5641 gcaaatcctt gcccaggtcg tccacgatcg cggccatgat gcccggcagt gccaaggtgc
     5701 acgaacccgg caccagcggg gttgcgcccg gcaacggcga gatcatgtgg ccgcccgttt
     5761 ccgtctgcca ccatgtgtcc accaccgggc agcgctcgcc gccgacgttc ttgtagtacc
     5821 acatccaggc ttcgggattg atgggctcgc ccacggtgcc caggatgcgc aggctcgaaa
     5881 ggtcgaagcg cgcagggtgg atcttctcgt cggcctcggc ggccttgatc agggaacgga
     5941 tcgccgtggg cgcggtgtag aacacgctga ccttgtgccg ctggatcatc tgccagaagc
     6001 ggcccgcgtt cggatatgtg ggaattccct cgaacatgac ttcggtgcag ccggccgcca
     6061 acggcccata agccacgtag gtatggcctg tcacccagcc gatgtccgct gtgcaccaga
     6121 acacgtcgga gggcttgatg tcgaacaccc agtccatcgt gagcttggcc cacaacagga
     6181 agccgccggt ggagtgctgc acacccttgg gcttgcccgt ggagcccgag gtgtagagga
     6241 cgaacagcgg gtgctcggcg cccacgggct ccggcctgca ctcggtggac tggccctgga
     6301 gcacttccgt gaacgtctgg tcgcgcccgg cgaccatgtt gcacggcgtt ggggtgcgca
     6361 tgaacaccag cacgctcttg agcgtgtggc agccgccgat gtcgatccct tcgtccacga
     6421 tggccttgag cggcatttcc ttgcccccgc gcatctggaa attggcggtg accacggcca
     6481 cggccccggc atcgatgatg cgctcgttca gcgctttcgc cgagaagccg ccgaacacca
     6541 cgctgtgggt ggcaccgatg cgcgcgcagg cctgcatggc cacgacggcc tcgatgcaca
     6601 tgggcatgta gacgacgacg cggtcgccct tgcggatgcc gtgcgccttc agggcattgg
     6661 caaactggca cacgcgtgcg agcagttcct tgtaggtgac ctcaaccacc gagctgtcgt
     6721 cggcctccca gatgatcgcc gtcttgttct cgacaggcgt gcccatgtgc ttatccaggc
     6781 agttggccga cgcattgagt tcgccgtcgt cgaaccactt gtagaacggg gcattcgatt
     6841 catccagggt cctggtgaag ggcttgctcc agaccacgtt ttcccgcgcc aggcgagccc
     6901 agaacccctc gaaatcgcgg ctggcttcgt cgcacagcgc gttgtaggcc tccatgccgg
     6961 aaatgcgcgc cgccttgacg gtggcctcgg aggggggaaa gacacggttt tcgaccagga
     7021 ccgactcgat agcactcatg tatgtctccg caaggtgatg aattccgctg ggtactgtca
     7081 gtgcttgcac ttacaagcca ctgacggccg gcaattcttg cgaatctacc cgaaccgggc
     7141 ctgccgggct ggaaatccac aatgccgcac atccgtaata acccgctgcc cgatgctaga
     7201 ccccaaccac ccgaaaaacc cccgctcgcc gctgcgtccc ctgcccggcc tgatcttcgc
     7261 cagccgccgg ctgcagttgc ctctgctcaa gaccttcatc aatgccgaca actactcgga
     7321 tcgcgtgctg atcgcccaga ccgccatcca catcaccttc ctgctgtcgg ccatggcgat
     7381 cgcctacacc gaccgcatca tgtcgggcat ccgcgtggag aaacattagg aattgtcatc
     7441 ccgggctggg ctcgcggaac gcccggggcg cgcagctgtc cagtgcagcg agcccaatgc
     7501 cgggcctacg ctatgcacgt accgaggcat cacgctccgg cccggccccg ggatccatgt
     7561 tggcgcgcgt gcatggatgc ccccggatca gcgtcccggg ggcaggctcc tcgagtccgg
     7621 catgacaccc ctttatttcc ccaccatctt ctccggccgc acccactggt cgaattgttc
     7681 cgcggtgacg aagccggtgg cgatcgccgc gtcgcgcagg ctggtccctt ccttgtgcgc
     7741 cttcttggcg atctgcgccg ccttgtcgta gccgatgtgg ggattgagcg ccgtcaccag
     7801 catcagcgag cggccgacga gttccgcgat gcgctcgcga ttgggctcga tgccggcggc
     7861 gcagtggtcg ttgaagctgg ccatgccgtc ggccagcagc cgcacgcttt gcaggaagtt
     7921 gtgggcgatc atgggacgga acacattcag ctcgaagttg cccgaggcgc ccccgaaatt
     7981 gatggccacg tcgttgccga acacctggca gcacagcatc gtcaacgcct cgctctgcgt
     8041 gggattgacc ttgcccggca tgatcgacga gcccggctcg ttttccggga tgttgatttc
     8101 gccgatgccg ctgcgcggac cgctggccag ccagcgcaca tcgttggcga tcttcatcag
     8161 gctcgcggcc agggttttca gcgcgccatg cgcgtgcacc agcgcgtcgg ccgcggccat
     8221 cgcttcgaac ttgttgggcg cggtgacgaa gggcaggccg gtgagccgcg cgagttccgc
     8281 cgccactttt tcggcatagc cttccggcgc gttcaagccc gtgcccacgg ccgtgccgcc
     8341 cagcgccagc tcgcacaggt gcggcaggct gccatgcagg tggcggtcgg cctgctccag
     8401 ctgggcggca tagcccgaaa actcctggcc cagcgtcagc ggcgtggcgt cctgcaaatg
     8461 agtgcggcct atcttgacga tgtcatggaa cgcgccggcc ttggcgccga gcgtggcctt
     8521 tagtttcatc agcgagggca tcaggcggtt gcggatggcg tccaccgccg ccacgttcat
     8581 cgccgtgggg aagacgtcgt tggacgactg gctcatgttc acgtcgtcgt tcgggtggat
     8641 cagccggccc tccccgcgcg ggccgcccag cagttcgctg gcgcggttgg ccagcacctc
     8701 gttgacgttc atgttggtct gggttcccga gccggtctgc cagaccacca ggggaaattc
     8761 atcgtcgtgc cggcccgcca gcacctcgtc ggccgctgcg atgatggcgg acgccttgcc
     8821 gtcgtccatc aggcccaaca ggtggttcac cacggccgag gagcgcttga tctggaccag
     8881 cgcccgcacg atctctcgcg gctggcgctc gccggagatg tcgaagttct gcagggagcg
     8941 ctgggtctgg gcgccccaca gccgctcggc cggaacctcg atcggcccga aagtatcgcg
     9001 ttcgatccgg atgttcatct tggatcaata cctgggtaag gttgggctgt caaaataccg
     9061 ctttgagagc caccgtaaca gagttgcggc ggcttgtgta ccccagtgtc ctcacccacc
     9121 cccgcatcat gaccaccacc atcatcaagc agagcgacct gaccgaatcg atcgcggccg
     9181 cgctccagta catcagctac taccaccccg cggacttcat cgcccacctg gcgcgcgctt
     9241 acgagcgcga acaaagcccg gcggccaagg acgcgatcgc gcagatcctc accaacagca
     9301 agatgagcgc gaccgggcac cgcccgatgt gccaggacac cggtatcgtg aacgttttcc
     9361 tcaaggtcgg gatggacgtg cgcttcgagg gcttcaccgg caacctggac gatgccatca
     9421 acgaaggcgt gcgccgcgcc tacaaccacc ccgataacac cttgcgcgcc tcggtcgtgg
     9481 ccgatccgca gttcgagcgc aagaacacca aggacaacac accggcggtg atcttcaccg
     9541 agctggttcc gggcaacaaa ctggatgtca ccgtggcggc gaaaggcggc ggcagcgaga
     9601 acaagagcaa gctggccatg ctgaacccga gcgactcggt ggtggactgg gtcctgaaga
     9661 cggtgcccac catgggcgca ggctggtgcc cgcccggcat actgggcatc ggcatcggcg
     9721 gcacggccga gaaggccatg ctgatggcca aggaatcgct gatggaccac gtggacatgt
     9781 acgagctgca gcagcgcggc ccgaaggaca agaccgagga gatgcgcctg gaactttacg
     9841 aaaaggtcaa tgccctgggc atcggcgccc agggcctcgg cggcctcacg acggtgctgg
     9901 acatcaagat caagatgtac cccacgcacg cggcaggcaa gcccgtggcg atgatcccca
     9961 actgcgccgc gacccgccat gcgcatttcg tgatggacgg ctcgggcccg gtgtacctgg
    10021 acccgcccag cctggacctg tggcccaagg tgcaatgggc tccggactac gagaagagca
    10081 ggaaggtcaa cctcgacgcc cttacgcgcg aagaagtcgc gagctggaag ccgggcgaca
    10141 cgctgctgct gaacggcaag atgctgaccg gccgcgacgc ggctcaccag cgcatccagg
    10201 acatgctggc caagggcgag aagctgccgg tggacttcac caaccgcgtc atctactacg
    10261 tcggcccggt cgatccgatc aagggcgagg ccgtgggccc tgcaggcccg acgacggcga
    10321 cccgcatgga caagttcacc gagatgatgc tggcgcagac cggcctgatc gcgatgatcg
    10381 gcaagtccga gcgtggcccg gtcgccatcg aggcgatcaa gaaacacaag agcgcctatt
    10441 tgatggcggt gggcggcgcc gcctacctcg tgtccaaggc catcaagacc gcgaaggtgg
    10501 ttggattcgg cgacctgggg atggaagcca tttacgagtt cgacgtggtc gacatgccgg
    10561 tgacggtagc ggtggacgcc ggcggcacca gtgcccacat caccggaccg gccgagtggc
    10621 agaagaagat cgccagcggg gagttcaaga gcatcccggt cgcggcagcg tgaagctgtc
    10681 atgccgggct tgacccggca tccgaggcgg ccgggaaggt ccggccggcg ggcgccgggc
    10741 gcgtggattt gctcggcacc ggctcgctgc ctgccctgcg ggaagccgcc gcacgctggc
    10801 tctaagccgc ggcggcgtcg gcctccagcg ccgcctcccg gccgcgccgt acattgaccg
    10861 gcaccaacaa caacaagccc gcgatgaaca gccccgccgt ggacatgatg gcgaggcgct
    10921 ggttgccgcc ggtggcccag gtgatggcgc cgtagctcaa gggaccgatg atgctggcca
    10981 gtcggatcgc gaaggtccac aggccataga actccgccag ctgattgcgg ggcgcgaaca
    11041 tcccggccat ggcgcggccg gccgactggc tggaccccat gcacaggccg gcaatcgctg
    11101 ccgcccacca gaaccctccc ttggtcgtgg tcaccgcggc aatgatgcag gtggcgaccc
    11161 agcccacgag cgtgctgccc agcgcgatct tgtgcccgat gcggtcctgc aggtagcccc
    11221 agccgaacgc cccggcggcg gcggcgatat tgagcacgaa aataaggacc atagtctcct
    11281 gttgctggaa cccgatcacg gtttccgcgt agatcgcggc cagcgtgacc gccacggcca
    11341 ccccgccctg gtagagcgcc gcgcacgaca gcagccacat gaaatcctcg aagcgcctcg
    11401 cctggccgaa ggtgcgatgc agttgctgca gggacgcctt gaggccgcct tgcctcagcg
    11461 ccgccgggtt gggccgcgcc ctttcaccga gcagccagaa ggtgaccagc gctgcgctcc
    11521 cgtacagcac ggccgtgatc agcatcgtga ccggcacgaa ccgctccgcc gcaatcccct
    11581 gcccctgcgc ccagatcacg tagcccaggc acaggccgag ggccatcatg ccgccgaaat
    11641 aaccgaagct ccagccccag ccgctgacct tgccgagcga gtccggacgg gccagctcgg
    11701 gcaggaaagc cgccgtgagc gattcgccgt acgagtaaaa ggtgttggag accacgatca
    11761 gtaacatcgc gagggccacg ctgccggggc cggccagcgc gagtgccgcc gtcgagacga
    11821 cacagccccc cgtgaacagg gccagcaggc gcttcttggc ggcacgcaga tcggcatagg
    11881 cgcctatgct gggcatggtc agcatcacga tggcatagga gacgctcagt gccgcggtcc
    11941 aggcaaacgt ggcccattcg gccttgcccg cgatgccgcc gacgaagtac gccgcgaaga
    12001 cggccgtgat caccaccgtg gtgtagcccg agttcgcgaa gtcgtacatg gcccagccga
    12061 acacctcgcg cttgcgcacg ccggggttca gcgcgtcgcg cgagaagagg ggcatgagtt
    12121 aggggctggg ggctgggggc tgggggttag gggcgaggag cctgggttgg gtggagtccg
    12181 gtatttccct agcccctagt ccctaatctc taatccctag cccctgcatt cctcaatgta
    12241 aatggcgagg ccgccgtccg ccgccgtgcc ccggaccgcc gcccaggccg cgcggcggct
    12301 tgccgatttc cagcgagttc accagcgcgt cgaatcgctg gctgaaaagc ttgtcgatct
    12361 cggcgaccac gccgcccagt tccgagccgt cgaaatggcg ctccatcagg ttcaccacgt
    12421 cctcgaccag caggtcgcgc tgcacctgca tgatggcggc cggcgtagga ccgacgaaca
    12481 gcatcacgaa gtcgtcgcgc gactccgctt cgggcgcttc ttcttcctcg tcgaactcgg
    12541 cgtcgtagaa agtcacctcg atggccgcgc cctgttccga cagctcgttc aggctcatgc
    12601 acatctcgtc gagcgattgg cggaagtcct cgtcgccggg cacagtccag cacatccgca
    12661 gcaagtgctc gggcgcgtcg aatttgatgc cgggctcctc ctcgtacgaa ctctcggcgc
    12721 cgtccgccag cgacttcgcg cccgcatagg tccacagcgg tttcagcgcg tcctgcagct
    12781 gggcatagct cacgtcgggg cgcagcggca cctcgccatg gacatggatc tcgaacggag
    12841 cgttatagcg tgacatggtg ctacccaaac tttctgctgc ctccagtggg catttgtcaa
    12901 ttctgcgcct ttggcgtgcc ggttcggctg ccgggcctcg gtggctgcct cgagccgaat
    12961 cgaaccggct tgccgcttgt caggcaagcg gcggatctta ggtcaacacc gcaaactaac
    13021 ataccctaag cacttgattt tgaacagaaa cgggtggttg agtcatgttg atttgtaggg
    13081 tttggcggtg tcgggtgatg gcttgttacg cacgaataac gcacggctat gatgccagag
    13141 tgcgcgggtg gcgaaactgg tagacgcagc gggcttaaaa cccgccgccc acccggaagg
    13201 gagcgtacgg gttcgagtcc cgtcccgcgc accatcactt cgaagtcccc ttggcggacg
    13261 tgacccccgg cccagcgccg gttcgccaag ccgacccgtc ccgcatcaat tgcacctttg
    13321 ctccgccctc gacctctgaa attcaccgca tgaaaaaatt cgtactgacc gccatcctcg
    13381 ctgccatcct gaccggatgc ggtggtggcg actccgatgc cccggcaggc ccggtagcgg
    13441 tgccccagag gctcgtcggg gaggtcatcg cctacgtgcc ccacgtgttg cagcaccgac
    13501 tcgaagcgtt caccaccgcc gccgccctgg ttcgcaagca catcccgcaa gcgagggtcg
    13561 gcgtcacgat cacgccttac gcgatgatcg ggcggacgaa cagcatggaa tacgccaaac
    13621 gggccatcgc cctgtccgat tgggtgggca ccgatcccta ctggttcggc ggcgacaacg
    13681 tgaaggaact caacgactgg tcgcacagct tcagcgcggt ggcgaagcaa gccaacccgg
    13741 cggtcgaaac ctggctcatc gcgcaggcgt tcaagatgcc cgagtgggac acggcaacgt
    13801 tcaatgcctt caccaaggag cagttggccg ccgccgaggg ctacgaccac atcctgttct
    13861 tcggctggca gttcgtgtcc gagatcgaca cgagcgcagc cgggatgcac ttcggggccg
    13921 agacgaaggc cctgttcagc aagtacctga aggcccagtg aatgcagccg cggtgacgac
    13981 cctgcgccgg caacgcaggt tgccggggct gcgaagctgg aggcgcgggg cggagtcgaa
    14041 ccgcccttca cggatttgca atccggtgca taaccgcttt gctaccgcgc catttgtgct
    14101 caagaaaaag ggaagccatc gcttcccttt cctgaaatat ggagcgggca aagagtctcg
    14161 aactctcgac ctcaaccttg gcaaggttgc gctctaccaa ctgagctatg cccgcattga
    14221 agccttcaat tatagcgcaa ctctttggcc cttcgccata accgctgctc agtgcttgac
    14281 cgatacccgc acgcccgccg gctgctcgcc atcgcccgcg atcggcgcgg cgacgggcac
    14341 ggcggccggc tcctcatccg gcagcggcgt cggattgcgc tccagtgcga cctccagcac
    14401 cttgtcgatc catttcaccg gcacgatctc cagcccgctc ttcacgttgt ccggaatctc
    14461 ctgcaggtcc ttggcgttct cctgcgggat caatacggtc ttgatgccgc cccgcaatgc
    14521 cgccagcagt ttctccttca ggccgccgat cgccgtgact tcaccgcgca gcgtgatctc
    14581 gccggtcatc gccacgtcgg cacgcaccgg aatgccggtg agcgcggaca cgatggccgt
    14641 ggtcatcgcg gcacccgcgc tcggaccatc cttgggcgtg gcgccgtcgg gcacatggat
    14701 gtgcatgtcc ttcttctcga acacttcgtc gggaatgccc aggcggcgcg agcggctgcg
    14761 caccaccgtg cgcgcggccg cgaccgattc cttcatcacg tcgcccagcg agccggtgct
    14821 ggtgatcacg cccttgccgg gcgtcagcgc ggcctcgatc gtgagcaggt cgccgccgac
    14881 ttcggtccac gccaggccga ccacctggcc cacctggttc tgctgctcgg cgcggccata
    14941 ggtgaacttg cgcacgccca ggaaatcgtt caggttctcc gccgtgacga cgacctgggg
    15001 ggtcatcttc ttgagctgca ggcccttcac ggtcttgcgg cagatcttcg acaggtcgcg
    15061 ctcgagcgag cgcacgcccg cttcacgcgt gtagtagcgg atgatgtccc ggatcgcttc
    15121 ctccgtcacc tcgagttcgc tctccttcac cccgttgttc ttcatctgct tgggcagcag
    15181 gtacttcaag gcgatgctgg tcttctcgtc ctcggtgtag cccgaaagcc ggatcacttc
    15241 catccggtcc agcagcgccg gcgggatgtt catcgaattg gaggtcgcga cgaacatcac
    15301 atcggacagg tcgaagtcga cctcgacgta atggtcgccg aacgtgtggt tctgctccgg
    15361 gtccagcact tccagcaagg cgctggacgg gtcgccccgg aagtccgttc cgagcttgtc
    15421 gatttcatcc agcaggaaca gcggattgcg cgtgccgacc ctggacaggc ttgacagcac
    15481 cttgccgggc aaggcgccga tgtaggtgcg gcggtggccg cggatctcgg cttcatcgcg
    15541 catgccgccc agcgccatgc gcacgtactt gcggccggtc gccttggcga tcgactggcc
    15601 cagcgacgtc ttgcccacgc cgggcgggcc gaccaggcac aggatgggcg ccttcagctt
    15661 gtccacgcgc ttctgcaccg cgaggtattc gagaatgcgg tccttgacct tttccaggcc
    15721 atggtgatcc tggttgagca cgtcctcggc gtggcccagg tcgtgcttga tcttggtctt
    15781 cttgctccac ggcagggcgg tgagcacctc gatgtagttg cgcaccacgg tggcctcggc
    15841 agacatgggc gacatcagct tgagcttctt gagctcgccg tcggccttct tcttggcctc
    15901 cttgggcatc ttggccgcct tgatcttctt ctcgatctct tcgatgtcgg cgccctcttc
    15961 gccctcgccg agttccttct ggatcgcctt gacctgctcg ttcaggtaga agtcgcgctg
    16021 gttcttttcc atctggcgct tgacgcggcc gcggatcttc ttgtcgacgt tcaggatgtc
    16081 gacctcgcgc tcgatctgtt cgaacagatt ctccagccgc agcttgacgt cggccaggtc
    16141 gagcacgatc tgcttgttgt cgagcttgag cggcaggtgc gcggcaatgg tgtcggccag
    16201 gcggccgggg tcgtcgatgc tggagatcga ggtgaggatt tccggcggga ttttcttgtt
    16261 cagcttgacg tactggtcga actgctgcat cacggcgcgc cgcagcgcct cgatctcgct
    16321 ggatttcggg tctttctcgg ccgattccac cggggtgacg ttggccgaga agtgctgctc
    16381 gccgtcctcg atgcggttga cacgggcgcg ctgctggcct tccaccagca ccttcacggt
    16441 accgtcgggc agcttgagca tctgcaggat ggtggagacg cagccgacct cgaacatgtc
    16501 ggagaccgac ggctcgtcct tggcggcggc cttctgcgcc accagcatga tgcggcgttc
    16561 cgcctccatg gcggcctcca gggccttgat gctcttgggc cggcccacga agagcgggat
    16621 gaccatgtgc ggaaagacca ccacgtcgcg cagcggcagc agcggcaggt cgatcgcggt
    16681 ggcgggcaat ggaatgtgtc cggacattgg gaatcctttt gtggccagtc agtcgaatat
    16741 ggcctggaat agtggatttt caacccgtcc gcatgagtat gcggctcgca gggtcgttat
    16801 tcttgtcgaa ggttaccagg gatgagccct aggccttctt ggccgcctca cggtagacca
    16861 gcaggggcgg cttgttttcg tcgatggtgg actcgtcgac caccaccttg tccacattgg
    16921 ccgtgttggg cagttcgaac atggtgtcga tcagcgactg ctccaggatc gagcgcaagc
    16981 cccgggcgcc ggtcttgcgc gccagcgcct tgcgggcaat ggccttgagc gcgttgggcc
    17041 ggatttccag gtccacgcct tccatggaca gcagcttgcc gtactgcttg accagggcgt
    17101 tcttcggctc cgtgaggatc cggaccaggg cgtcctcgct cagctccgac agcgtcgcga
    17161 cgaccggcat ccgccccacc agttccggga tcaggccgaa cttgatcagg tcttccggct
    17221 cgacctcgcg gaatgcgtcg ctgaggctgc gctgggcctt gctcttcacg gccgccccga
    17281 atccgatacc ggatgcttca gtgcgctgct cgatcacttt ttccagaccg gagaaggcgc
    17341 cgccgcagat gaacaggatg ttggtggtgt ctatctgcag gaagtcctgg ttggggtgct
    17401 tgcgcccgcc ctgcggcggc acgctggcca tggtgccttc gatcagcttg agaagggcct
    17461 gctgcacccc ctcgcccgac acgtcgcggg tgatggacgg gttatcggac ttgcgcgaga
    17521 tcttgtcgat ctcgtcgata tagacaatgc cgcgctgggc ccgctcgacc tcgtagttgc
    17581 agctttgcag cagcttctgg atgatgttct cgacgtcctc gccgacgtag ccggcttcgg
    17641 tcagcgtcgt cgcgtcggcc atcacgaagg ggacgtccag catgcgtgcc agcgtctgcg
    17701 ccaacaaggt cttgccggag ccggtggggc cgatcagcag gatgttgctc ttggccagct
    17761 cgacttcgtc cttggcgcgc tgttcggagg tcttggtctg caggcgcttg tagtgattgt
    17821 agaccgcgac cgacaggacc ttcttcgccc ggttctggcc gatgacgtag tcgtcgagga
    17881 cgcgcttgat ttcctgcggc ttggggagct tctcgcgggt cttctccgcc ttctcctcga
    17941 gctcctcacg aatgatgtca ttgcagagct cg
//
LOCUS       DASWIY010000413        16406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2155543, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000413 DASWIY010000000
VERSION     DASWIY010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 16406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16406
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2155543"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..489)
                     /locus_tag="VGA59_14555"
     CDS             complement(49..489)
                     /locus_tag="VGA59_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720938.1"
                     /translation="MTSFAHVDYPTEHAGVVRAERAAQSLQKAVHGFNGTRAGASFLL
                     AAIVAALVVIASQVIDTWTEGHLLAAWIVMWAVVFAALALFAAPAKRAAAALRVGLQR
                     WNAARKRRAEDDQLWRLALTDARVMADISRAMTEGATREIKAYY"
     gene            605..1576
                     /locus_tag="VGA59_14560"
     CDS             605..1576
                     /locus_tag="VGA59_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9720939.1"
                     /translation="MKATPNFRTLDLNLLRVFDQVMAERNLTRAARNLAMTQPAVSNA
                     LNRLRDAVGDKLVARSGYGVEPTPRALALWPAIADALRQMEASLTPGEFVASEAATTF
                     VLGMADATAAELVPGLVEIIERDAPGVSMRVLPLTTRDPRRLLDEGHMDLAAGFFPLV
                     LADLTAQSQGGGMAPFDHQRLFAGEYVCVMRKGHPLASGPLTVKRYCAARHLLVSFSG
                     RPYGMVDEALASLGRKRRVVITANQFFTAGRVVAGSDLLTVLPRHFVNITGMAHEFVL
                     RDLPFDVPPVLVDSLWHRRQGQRSDHAWLRLAVAAAAHKAFGDGRAW"
     gene            1652..2470
                     /locus_tag="VGA59_14565"
     CDS             1652..2470
                     /locus_tag="VGA59_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9720940.1"
                     /translation="MKIQLLSDLHLEVHPHYCPRPAPEAQLLVLAGDIGSYQDGSLLA
                     GDDFGLERFSPRLGWPVPVLYVPGNHEYDNLDFDSAHARLRETCDRLGITWLEREVLT
                     LDQVRFVGTTLWADFDALVTDSDRADTTRVLKKREKAFRAANYYLQKTGTTRRGEPML
                     APAWREQGLVCEHWLRRALAEPFDGTTVVVTHFAPSLRSADPRYGLTPGTAGFCNSLD
                     ELLPLAQLWLHGHLHHQQDYVAGGCRVVANTLGYAARGEQEGFREQLVLQVPVP"
     gene            2645..3199
                     /locus_tag="VGA59_14570"
     CDS             2645..3199
                     /locus_tag="VGA59_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720941.1"
                     /translation="MTNTIWTPTLAWLAAATGALLLAMAAPDESRLLGQLPTLTVKRL
                     DQQRFVLPQGLLAQRTLALVAFHPNQRDELRSWIRGLRLDQDRSITWFKMPVLSDPGS
                     ERARSDIESVLLAGHASEIDRSRLVPVFTDRDAFIRSAGLSGTEHAWVLVLDRDGKVL
                     ARAEGKFDPDKADALRETLRAEGN"
     gene            complement(3215..4153)
                     /locus_tag="VGA59_14575"
     CDS             complement(3215..4153)
                     /locus_tag="VGA59_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HEX9720942.1"
                     /translation="MSTFDPDALECLAAIVEEGGFERAAQRLSITQSAVSQRLRALEA
                     QVGTVLIVRSRPLKPTAAGQLLLKHTKQMRLLRADLERDLKELAPSSTGGAREEERIS
                     IAINADSIATWALPALNELARQGLPMEIIADDQDFTQERLREGQVLGCVTTLKQALRG
                     CKVVPLGAMSYIAVAEAQYAAQHFPQGLSQHNFRDAPFIAFNRKDDMQSEFVGRAFGL
                     KRVALNQLFVPSSEGQVRAVLAGWGVSVVPELLARGLLEQGRLANIAPAHTLPIQLFW
                     HCWNLESEVLDALTGALTQAANASLTQATDAALAEV"
     gene            4231..5556
                     /locus_tag="VGA59_14580"
     CDS             4231..5556
                     /locus_tag="VGA59_14580"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEX9720943.1"
                     /translation="MKVLVLGAGIIGTSTAWHLLDRGHEVTVVDRQASAAQEASFANA
                     AQISVSYCEPWANRQAPAKLIKWMFRNDAPLLFRPQADWQQWRWGLQFLAQCNDSAFE
                     RNVQQLVALGGYSHAALNELVRATGIEYQRLQCGIAHYYTDQASFEDAGQAAELMRKF
                     GVHRKVVSREELLAIEPAFKSFADRIAGGTYTPSDETGDALVFTEQLAARCKARGARF
                     LFNHDVERLDKAGGSIEAVAVRDRATGAARKLKADEVVVACGSYSAPLLRTVGVDLPI
                     YPGKGYSATFKLKRPELAPRVSAIDDAVKIAMSRLGDELRVAGTIELAGYDLSLDTPV
                     ARARCRMLAERIEQVLPGVCDTRSAEEGGNPRFWTGLRPATPTNIPYIGRTRVGRLWV
                     NAGHGTLGWTHGAGSGKAMAQLISGQRPDLAFDFYGIEPEQQRLSPSPA"
     gene            complement(5618..6409)
                     /locus_tag="VGA59_14585"
     CDS             complement(5618..6409)
                     /locus_tag="VGA59_14585"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9720944.1"
                     /translation="MKEQKPFFERRRERRRLLQTGLAVAAGGALAGCDALSHNDTAVQ
                     VLKSAETLTQAVHRVLGRRAMAQEFSPGDIAPTFRANGTTMPLGDAYAALMAKGFADW
                     RLKVDGLVARPLALSLAQLRAMPARTQITRHDCVEGWSVIGQWKGVRLSHLLNLAGVK
                     PQAMFVIFHCLDRMDKDDERSLYYESVDLDDALHEQTILAWELNGKPVPVENGAPLRA
                     RIERQLGYKHAKYLHRVELVAGLEKTAGGQGGFWEDRGYNWYAGI"
     gene            complement(6406..7221)
                     /locus_tag="VGA59_14590"
     CDS             complement(6406..7221)
                     /locus_tag="VGA59_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX9720945.1"
                     /translation="MEQVSTGNPPLVYRHSLPVRVMHWVNVVCLVVLLMSGLQIFNAH
                     PALYWGHDSRDSTRILQITQKQVAGEWRGVTRVGAAEFDTHGILGTSRSDDGDYAARA
                     FPAWATVPGPQWLAMARLWHFFFAWIFVANGIAYVAWTVFSGHLRRDLVPTHSEWRGI
                     GRSIRDHLLLRHPHGESARRYNVLQNVAYLAIVFGVLPLIVVGGWAMSPMMDAFVPGW
                     VDWLGGRQAARTLHFIAAVLLVAFVLVHVFEVIVAGLVNNLRSMITGYWRVPK"
     gene            complement(7319..8593)
                     /locus_tag="VGA59_14595"
     CDS             complement(7319..8593)
                     /locus_tag="VGA59_14595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9720946.1"
                     /translation="MPAMSFTPAVGDLPFTAKQRSLLSLANELGRTRFAPRAAQWDEA
                     ASFPFANYDDLREAGLLALCVPQSHGGLGADFASYMMVAAEIGRFCGATALTYNMHIC
                     STLWTGVLADGIAMTDRQRAEHARRRELHFKRVVHDGAIYAQPFSEGSAAAAGRAPFG
                     TTARKVEGGWVVNGRKIFASLSGAADFYGVLCTEDKGDEKPDVRDTLYLAVPATSEGF
                     SIQGDWNPLGMRGTVSRNLAFKEVFVSDDEQLMPRGVYYVGAQTWPAMFFTLAPTYLG
                     LANAAYDFTVAYLRGEINEATGEPSRPGERRTVRRMYPTKQIAVAQMRIQLEAMKSLF
                     ARVIQEAAPNPSQDARMRLYAAQYSVMEGANDIARLAIRTCGGQSMMRHLPLERIYRD
                     SRCGSLMLPWTAELVLDRMGRETLYEPGEKDE"
     gene            complement(8619..9452)
                     /locus_tag="VGA59_14600"
     CDS             complement(8619..9452)
                     /locus_tag="VGA59_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9720947.1"
                     /translation="MNVPSFTVLGSGPTILMLHGIGGGHLAFAPQVETLASSGYRAVA
                     WDMPGYGRSAPIEPYTFKGLARSCITLIEALLAGDEKGSAAGTANNARRDGVILLGHS
                     MGGMVAQEVVARRPELVSRLILCGTSPSFGKAEGNWQRKFIAERTAPLDAGKSMADLA
                     DVLVPQMVGPASLPEGVRLATHCMSQIPASTYRRALEALVTFDRRANLSHIQVPTLLV
                     AGELDHNAPPAVMKKMAEAIPNSTYLEMRGIGHLQNLEAPDDFDGLVLNFLALPRALL
                     H"
     gene            complement(9598..10665)
                     /locus_tag="VGA59_14605"
     CDS             complement(9598..10665)
                     /locus_tag="VGA59_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M29"
                     /protein_id="HEX9720948.1"
                     /translation="MLQETIEPAWVDAFEAVLARCALRPGDTVAILSESQSRPVLPQL
                     ARLAAARLGCRSFQISVPTLFDAAGPPVRSTGASTAVQQLAPVIAALAASTLVVDCTV
                     EGLMHASELPAILRGGARVIYVSNEHPEALARLVPGDALEPLVKDHMKRLRSARSMHV
                     TSAAGTDLAISLVGATCGGNWGYTTRPGTMTHWPGGLALAFPAAGSVNGTLVLAEGDV
                     NLTFKRYIERPITLLVENDYVTKIDGAGVDAELMRSYIAAWSDLEAGSENNAGRDAYA
                     VSHVGYGLNAAARWDSMALYDKRDFNGTELRAFAGNFLYSTGANEVAGRYTRGHFDLP
                     LRNCTVELDGVAVVDAGKVVR"
     gene            10715..11224
                     /locus_tag="VGA59_14610"
     CDS             10715..11224
                     /locus_tag="VGA59_14610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9720949.1"
                     /translation="MSPSRKAQPPSFSAHEFRNALGMFATGVTIVTARTSDGRVIGLT
                     ANSFNSVSLDPPLVLWSLSQAAASLPAFRAGSHYAINVLAAEQKALAERFALKGTDRF
                     AGVSFVEGTGGAPLLAGAAATFECFNRSRYEEGDHVIFVGEVERCSWRSGAMPLLFHG
                     GQFYTELPI"
     gene            11221..11667
                     /locus_tag="VGA59_14615"
     CDS             11221..11667
                     /locus_tag="VGA59_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9720950.1"
                     /translation="MKKVPDMGKTRFVDSYLGYLLGQANHALYKDFDAHVRAAGLSSI
                     EWRVLATLHDSEPLTIGQLANEVLSKQPTVTKLVQRMAEQGWVALHADASDQRCTRVT
                     ATAAGKRLVTPLVDEARQQEARILRPLAATEKVALRKLLEKLAQHT"
     gene            11809..12708
                     /locus_tag="VGA59_14620"
     CDS             11809..12708
                     /locus_tag="VGA59_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9720951.1"
                     /translation="MRERKQHLDALAISLLLACCLFWGFQQILIKTTVGEVPPLWQAA
                     LRFVGATALLWLWCAARGVKLFQRDGTLRAGLLAGVLFAGEFACIYLGLRDTTASRLT
                     VFLYTSPFVVALLLPRLVPSEKLRVAQWSGLLIAFAAVAVAFSEGFSGGTTSRQLRGD
                     ALGLGAGILWGLTTLVIRASALSTASAEKTLFYQVAVTALVAPLLSLALGEAWSLDYS
                     AYAWGSLALQTAIGAFASYLAWMWMLRHYPATRMSSFTFLTPVFALVLGVALLKEPLT
                     LQLMLALGAVAVGIVLVNRRPAA"
     gene            complement(12859..13671)
                     /locus_tag="VGA59_14625"
     CDS             complement(12859..13671)
                     /locus_tag="VGA59_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9720952.1"
                     /translation="MSDITPRIDFSQELAGFFSLKGKTAYLPGGYGGIGEAMAWALAL
                     AGAKVAVSGRDPARAEALAGELKAAGHEALGLAMDAHSVAQIQSSVDRVAAELGGVDI
                     LVNCVGMQREQALLEVTEETFDEVVQVNLKAAMFLAQAAARHQIAAGRGGAQIHLLSV
                     RARLGLRGRGYSAYCGSKGGLVMLIRQHAGELAPHGVTVNGIAPTVVRTEMARHWLEN
                     PDTRAQVLGRIPMGRVADPKDVAGAAVFFASPAASFITGQVLYVDGGITASQ"
     gene            complement(13674..14417)
                     /locus_tag="VGA59_14630"
     CDS             complement(13674..14417)
                     /locus_tag="VGA59_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9720953.1"
                     /translation="MRALEGRIAVVTGAAGTMGLAATKFLLEDGCKVAMVDANGQRNE
                     ELAAQLGANARAFCFDISDPKAVREGHERIVATLGEADILLNNAGILSNNKAEATDTA
                     EWRRVLGANLDGAFFLAQRVMPAMKARRFGRIINTCSLAAKTGGLTAGTAYSVSKGAL
                     TSLTFSLARELAPYGVTSNGISPAYVKTPMITEQLNEDQRQRLLKDIPVGRFCEPEEF
                     AHVVRFLASPLAGFITGEIVDLNGGLIMD"
     gene            complement(14584..15495)
                     /locus_tag="VGA59_14635"
     CDS             complement(14584..15495)
                     /locus_tag="VGA59_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9720954.1"
                     /translation="MLPHLLNGLSLGLLFALIALGFMLIVGVMETINLAHGSLFALGM
                     YFALFFVSPRLGWFPELQAWYMALPIDTRYLIALFLAPVFVGLFGMLLELCLRRTYGR
                     DPLYGLLLTFGAALVIEETIRVVWGSTEKQLALPQAISGAFLLGDLIYSKYRFFASGF
                     AILAIAALWLFLEKTPYGAIIKAGAHDSEMVRALGINLSRLRLLVFGLGVALAAIAGV
                     VMAPIWGIRPHVGVDAVVPAFLIIVLGGVGSLWGAVIAGLMVGMVVGLTGAYASEWSL
                     MSMYLLFIAVVTFRSRGLFGKKSVLDA"
     gene            complement(15672..>16406)
                     /locus_tag="VGA59_14640"
     CDS             complement(15672..>16406)
                     /locus_tag="VGA59_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9720955.1"
                     /translation="NDYVWGHNTSKATRAVVEANGGKIIDELMVPQNTRDFSAYLLKL
                     QQMKPQVVATAVGGDDIKALRQQVVQLKLHQNMAWINNQQDWPDVYGLGPEQIFGVFG
                     TTWYWRLQLPGVKEFVAKYQKQFPGMAIRVPGNVYHNGYMATRELLRCVEEAGTTNNL
                     AVIKKLEGRKMTAADRLQHHDAWIDPVTHHCQQTIYMAAYNDKPAEKDDIFKILTEVD
                     PKDVQDKDAPNACKLESYEATPSYEV"
BASE COUNT         2658 a         5560 c         5445 g         2743 t
ORIGIN      
        1 ggctcagcaa gccgggttga actagcgtgg tcattttttc gctccgtctt agtagtaggc
       61 cttgatctcg cgcgtcgcgc cttcggtcat cgcccggctg atgtcggcca tgacgcgcgc
      121 gtcggtcagc gcgagccgcc agagctggtc gtcttcggcg cggcgcttgc gcgcggcgtt
      181 ccagcgctgg aggccgaccc gaagtgcggc ggcggctcgc ttggccggcg cggcgaacag
      241 tgccagcgcg gcgaacacca ccgcccacat cacgatccag gcggccagca gatgcccttc
      301 ggtccaggtg tcgatcacct ggctggcgat caccaccagg gcggccacga tggctgcgag
      361 caggaagctg gcgccggcac gggtaccgtt gaagccgtgt acggcttttt gaaggctttg
      421 tgccgcacgc tcggcgcgaa ccacacctgc gtgttcggtc ggatagtcga catgggcaaa
      481 gcttgtcatc atttttcccc ttctagacgc tgcggctgga ttgccggagt gcgtagtcga
      541 atactagggt taatactgat atttttccaa tttcgattgg tgatggctat cattcacggc
      601 aatgatgaaa gccaccccca acttccggac cctggacctg aacctgctgc gcgtgttcga
      661 ccaggtgatg gccgagcgca acctcacgcg ggcggcacgc aatctggcga tgacccagcc
      721 ggccgtgagc aacgcgctga accggctgcg cgacgcggtg ggcgacaagc tggtcgcgcg
      781 cagcggttac ggcgtcgagc cgaccccgcg cgcgctggcg ctgtggcccg ccatcgccga
      841 tgcgctgcgc cagatggagg cctcgctcac gcccggagag ttcgtcgcct cggaagcggc
      901 caccactttc gtattgggaa tggccgatgc gacggccgcc gaactcgtgc cgggcctggt
      961 cgaaatcatc gagcgggacg cgcccggcgt ctccatgcgg gtgctgccgc tcaccacgcg
     1021 cgacccgcgc cggctgctgg acgaaggtca catggatctg gcggcggggt ttttcccgct
     1081 ggtgctggcc gacctcacgg cccaaagcca gggcggcggc atggcgccgt tcgatcacca
     1141 gcggctgttc gccggcgaat atgtgtgcgt gatgcgcaag ggccatccgt tggcctccgg
     1201 gccgctcacc gtcaagcgct attgcgcggc ccgccacctg ctggtgagct tctcagggcg
     1261 cccctatgga atggtcgacg aggcgctggc gtcgctggga cgaaagcggc gcgtcgtgat
     1321 caccgcgaac cagttcttca cggcgggccg ggtcgtggcg ggttcggact tgctcacggt
     1381 gctgccgcgc cacttcgtga acatcaccgg catggcccac gagttcgtac tgcgcgatct
     1441 gccgttcgac gtgccgccgg tgctcgtcga ctccctctgg caccgccgcc agggccagcg
     1501 cagcgaccat gcctggctgc ggctggccgt ggcggcggcg gcacacaagg ctttcgggga
     1561 tggccgcgcc tggtgagggg atttggcacg gcgcttgcgt tcgggcgtgg tgccgctcgg
     1621 catcgacgcc ggcgtcggca caatggcgcg gatgaagatc cagctcctgt ccgacctgca
     1681 cctggaagtg catccgcact actgcccgcg gccggcgccc gaggcgcagc tgctggtgtt
     1741 ggcgggggac atcggttcct accaggacgg cagcctgctg gccggcgacg atttcgggct
     1801 ggagcgcttc tcgcccaggc tcggctggcc ggtacccgtg ctgtatgtgc cgggcaacca
     1861 tgaatacgac aacctcgatt tcgactcggc ccatgcgcgc ctgcgcgaaa cctgcgaccg
     1921 gctgggcatc acctggctgg aacgcgaagt gctgacgctg gaccaggtgc gattcgtcgg
     1981 caccacgctc tgggccgact tcgacgcgct ggtgacggac agcgaccgcg ccgatacgac
     2041 gcgggtgctc aagaagcgcg agaaggcttt ccgcgccgcc aactattacc tgcagaaaac
     2101 cggcacgacg cgacgcggcg aacctatgct ggcgccggcg tggcgcgaac agggactggt
     2161 gtgcgagcac tggctgcgcc gggcgctggc cgagccgttc gacggcacca cggtggtcgt
     2221 gacgcatttc gcgcccagcc tgcgcagcgc cgacccgcgt tacggcctca cgccgggcac
     2281 cgccggattc tgcaattcgc tcgatgagct gttgccgctg gcacagctgt ggcttcacgg
     2341 ccacctgcac caccagcagg actacgtcgc cggcggctgc cgcgtggtgg cgaacacatt
     2401 gggctacgcc gcccgcggcg agcaggaagg ctttcgcgag cagctggtac tgcaagtgcc
     2461 cgtgccttga accggtgcgc cgcgcactcc atgccggtgc gcgcgagggc gcgatgcata
     2521 acgatgcaga aaagttgtga ggtattcacg ttcacgtaca tctgccctca ctactttaca
     2581 agccgtacgt gacggccggc tgggcgccat ggaacactgg gtcgcagcac ccgggaggcg
     2641 cgtgatgacg aacacaatct ggaccccgac cctcgcatgg ctcgcggccg cgaccggcgc
     2701 gctcctgctc gcgatggcgg ccccggacga gtcgcgcttg ctgggccagc tgcccacgct
     2761 gacggtcaag cggctggacc agcaacgctt cgtattgccg cagggcctgc tggcgcaacg
     2821 cacgctggcg ctggtcgcgt tccaccccaa tcagcgcgac gaactccgaa gctggatacg
     2881 cggcctgcgg ctggaccagg atcgctcgat cacctggttc aaaatgcccg tcctgagcga
     2941 cccgggaagc gaacgcgctc gcagcgacat cgaaagcgtg ctgctcgccg gccatgcgtc
     3001 ggagatcgac cgctcgcgcc tggtgccggt gttcaccgat cgcgacgcat tcatccgctc
     3061 cgccggcctt tcgggcaccg agcacgcctg ggtgctggtg ctcgatcgcg acggcaaggt
     3121 gctggcgcgg gccgagggca agttcgaccc ggacaaggcg gacgcgctgc gcgagacgct
     3181 gcgcgccgaa ggcaactagg agccgctggg gccgctagac ctcagccagc gcggcgtcgg
     3241 tggcctgcgt cagcgacgcg tttgcggcct gtgtgagcgc gccggtcagc gcgtcgagca
     3301 cctcggattc caggttccag caatgccaga acagctggat cggcagcgtg tgcgccggcg
     3361 cgatgttcgc cagccggccc tgctccagca aaccgcgcgc cagcagctcg ggcaccacgc
     3421 tgacgcccca tccggccagc accgcgcgca cctgcccttc cgagctgggc acgaacagct
     3481 ggttgagcgc cacccgcttc aggccgaacg ccctgccgac gaactcgctc tgcatgtcgt
     3541 ccttgcggtt gaaagcgatg aacggcgcat cgcggaaatt gtgctggctc aagccctggg
     3601 ggaaatgttg cgccgcgtac tgcgcttcgg ccacggcgat gtaagacatc gcgcccaggg
     3661 gcacgacctt gcagccgcgc agcgcctgct tcagtgtcgt cacgcagccc aggacctggc
     3721 cctcgcgcag gcgttcctgg gtgaagtcct ggtcgtcggc aatgatctcc atcggcaggc
     3781 cctggcgcgc cagctcgttc agcgccggca gggcccaagt ggcgatgctg tcggcattga
     3841 tcgcgatgga gatgcgctcc tcttcgcgcg ccccgccggt ggagctgggc gccagctcct
     3901 tcaggtcgcg ttccaggtcc gcgcgcagca gccgcatctg cttggtgtgc tttagcagca
     3961 gctggccggc cgccgtgggc ttgagcgggc ggctgcgcac gatcagcacg gtgcccactt
     4021 gcgcttccag ggcgcgcagc cgctgggaga cggccgattg ggtgatcgac aggcgttgcg
     4081 cggcgcgttc gaaaccgcct tcttcaacga tggctgcaag gcattccagc gcgtccggat
     4141 cgaaagtact catattaggc aacctgatgt tttcactgat agtttaattg cacttctatt
     4201 cgaatacaac ttccgtcaaa cttcctgccc atgaaagtac tcgttctcgg cgccggaatt
     4261 atcggcacaa gcaccgcctg gcacctgctg gaccgcggcc acgaagtcac cgtggtcgac
     4321 cggcaggcct cggcggccca ggaagccagc ttcgccaacg cggcgcagat ttcggtgagc
     4381 tattgcgaac cgtgggccaa ccggcaggca ccggccaagc tgatcaagtg gatgttccgc
     4441 aatgacgcgc cgctgctgtt ccggccgcag gccgactggc agcaatggcg ctgggggctg
     4501 cagttcctcg cccagtgcaa cgacagcgct ttcgagcgca acgtgcagca actcgtggcg
     4561 ctgggcggct acagccatgc cgcgctgaac gagctggtgc gcgccaccgg catcgaatac
     4621 cagcggctgc aatgcggcat cgcccattac tacaccgacc aggcttcgtt cgaggatgcg
     4681 gggcaggccg ccgagctgat gcgcaaattc ggcgtgcacc gcaaggtggt gagccgcgag
     4741 gagctgctgg cgatcgagcc ggcgttcaag tccttcgcgg atcgcatcgc gggcggcacc
     4801 tacacgccca gcgacgagac cggcgacgcg ctggtgttca ccgagcagtt ggcggcacgc
     4861 tgcaaggcgc gcggcgcccg gttcctgttc aatcacgatg tcgagcgcct cgacaaggcc
     4921 ggcggttcga tcgaggccgt cgcggtgcgg gaccgcgcca ccggcgcagc gcgcaagctc
     4981 aaggccgacg aggtggtggt tgcctgcggc tcttatagcg cgccgctgct gcgcacagtg
     5041 ggcgtggacc tgccgatcta tccgggcaag ggctatagcg cgaccttcaa gctcaagcgc
     5101 ccggaactgg cgcctcgcgt cagcgccatc gacgatgcgg taaagatcgc gatgagccgg
     5161 ctcggcgacg agctgcgcgt ggccggcacc atcgaactgg ccggctacga cctgtcgctc
     5221 gacacgccgg tcgcgcgggc gcgctgccgc atgctggccg agcgcatcga acaggtgctg
     5281 cccggtgtct gcgacacccg cagcgccgag gaaggcggca acccgcgctt ctggaccggc
     5341 ctgcgccccg cgacgccgac caacatcccc tatatcggcc gcacccgggt cgggcggctg
     5401 tgggtgaacg ccggccatgg gacgctcggt tggacccacg gtgccgggtc gggcaaggcc
     5461 atggcgcagc tgatcagcgg tcaacgcccc gacctggcgt tcgacttcta cggtatcgag
     5521 ccggagcagc agcgcctgtc gcccagtccc gcctgacgac gcggcatgac cgggcgggcg
     5581 cgccgggagt ctgcgcgata ggcgacttcg agccgcctca gatccccgcg taccagttgt
     5641 agccgcggtc ttcccagaag ccaccctgtc cgccggccgt tttctccaag ccggccacca
     5701 gctccacccg gtgcaggtac ttggcgtgct tgtatcccag ctggcgctcg atccgcgcgc
     5761 gcagcggcgc gccgttctcc accggcaccg gcttgccatt gagctcccag gccaggatgg
     5821 tctgctcgtg caacgcgtcg tccaggtcca cgctctcgta gtaaaggctg cgctcgtcgt
     5881 ccttgtccat ccggtccagg caatggaaga tcacgaacat ggcctgcggc ttgacccccg
     5941 cgagattcaa cagatgcgac aggcgcacgc ccttccattg cccgatcacg ctccagcctt
     6001 ccacgcagtc gtgccgcgtg atctgggtgc gcgccggcat ggcccgcagc tgcgccaggc
     6061 tcaacgccag cggccgggcc acgaggccgt cgaccttcag gcgccagtcg gcaaagccct
     6121 tggccatgag cgcggcgtag gcatcgccca aaggcatggt ggtgccattg gcgcggaacg
     6181 tcggcgcgat atcgccgggg gagaactcct gcgccatggc gcggcgcccc agcacccggt
     6241 gcactgcctg ggtcagcgtc tcggcgctct tgagaacctg caccgccgtg tcgttgtggc
     6301 tgagcgcgtc gcaacccgcc agcgcgccgc cggcggccac cgccaagccc gtttgcagca
     6361 ggcggcggcg ctcgcggcgc cgctcgaaga acggcttttg ctccttcatt ttgggaccct
     6421 ccagtagccc gtgatcatcg atcgaaggtt attcacgagc ccggcgacga tgacctcgaa
     6481 cacatgaacc aggacgaacg ccaccagcag cacggcggcg atgaagtgca gggtgcgcgc
     6541 cgcctgccgt ccgcccagcc agtcgaccca gcccggcacg aaggcatcca tcatcggcga
     6601 catggcccag ccgccgacca cgatcagcgg cagcacgccg aagacgatgg cgaggtaagc
     6661 gacgttctgc aacacgttgt agcggcgcgc cgactcgccg tgcggatggc gcagcagcag
     6721 gtggtcgcgg atcgagcggc cgatgcctcg ccattcgctg tgggtgggca ccaggtcgcg
     6781 gcgcaggtgg ccgctgaata ccgtccacgc gacataggca atgccgttgg cgacgaagat
     6841 ccaggcgaag aagaaatgcc acagcctggc catggccagc cactgcggac cgggcacggt
     6901 cgcccacgcg gggaaggccc gcgcggcgta gtcgccgtcg tcgctgcgcg acgtgcccag
     6961 gatcccatgc gtgtcgaact cggccgcgcc aacccgggta acgccgcgcc actcgccggc
     7021 cacctgcttc tgggtgatct gcaggatgcg cgtgctgtcg cgcgagtcat ggccccagta
     7081 cagggccgga tgcgcattga agatctgcag gccgctcatc aggagaacca ccaggcacac
     7141 cacattgacc cagtgcatca cgcgtaccgg caagctgtgc cggtaaacca gcggcgggtt
     7201 gcctgtcgaa acctgctcca tcggacctcc ggagggggga gtgcgtgaca cgagactacg
     7261 ccgaaacggc gttccatgag ttctgtcgcc agggctgcgg ttttcttaca cgcccggttc
     7321 actcatcctt ttcgccgggc tcgtacagcg tttcgcggcc catgcggtcg agcacgagct
     7381 cggcggtcca gggcagcatc agcgagccgc agcggctgtc gcggtaaatg cgctccaggg
     7441 gcaggtgcct catcatgctc tggccgccgc aggtgcggat ggccagccgc gcgatgtcgt
     7501 tggccccttc catcacgctg tactgcgccg cgtacagccg catgcgggcg tcctgcgacg
     7561 ggttcggcgc ggcctcctga atgacgcgcg cgaaaaggct tttcatcgcc tcgagctgga
     7621 tgcgcatctg cgccacggcg atctgcttgg tcgggtacat gcggcgcact gtccttcgct
     7681 cgccggggcg cgacggctcg cccgtcgctt cgttgatttc gccgcgcagg tacgccaccg
     7741 tgaagtcgta cgccgcgttg gccagcccca ggtaggtggg cgcgagcgtg aagaacatcg
     7801 ccggccaggt ctgcgccccc acgtaataga cgccgcgcgg catgagctgc tcgtcgtcgg
     7861 agacgaacac ctccttgaag gccaaattgc ggctcaccgt gccgcgcatg cccagcgggt
     7921 tccagtcgcc ctggatggaa aagccttcgc tggtggccgg cacggccagg tagagcgtgt
     7981 cgcgcacgtc gggtttctcg tcgcccttgt cctcggtgca caaaacgccg taaaagtctg
     8041 ctgcgccgga cagcgaagcg aagatcttcc gcccgttcac cacccagccg ccctcgacct
     8101 tgcgcgccgt ggtgccgaag ggcgcgcggc cggcggcggc ggcgctgccc tcggagaacg
     8161 gctgcgcgta aatcgcgccg tcgtggacga cccgtttgaa atgcagctcg cgccggcgcg
     8221 catgctcggc gcgctgccgg tccgtcatgg cgataccgtc cgccagcacg ccggtccaca
     8281 aggtggagca gatgtgcatg ttgtaggtca gcgccgtggc cccgcaaaag cgcccgatct
     8341 cggccgcgac catcatgtag ctggcaaagt cggcgcccag cccgccatgg gattgcggca
     8401 cgcacagtgc cagcaggccg gcctcgcgca ggtcgtcgta attcgcgaac gggaaactcg
     8461 ccgcttcgtc ccactgggcc gcgcgcggcg cgaaccgggt gcggcccagc tcgttcgcca
     8521 gcgatagcag cgatcgctgc tttgccgtga acggcaggtc gcccacggcg ggcgtgaagc
     8581 tcatggcagg caaggcattc atcaagatcc ttcgggtgtc aatgcagcag cgcgcgcggc
     8641 agcgccagga aattgagcac cagcccgtcg aagtcgtcgg gcgcttccag gttctgcagg
     8701 tggccgatac cgcgcatttc caggtaggtg ctgttcggga tcgcctccgc catcttcttc
     8761 atcacggccg gcggcgcatt gtgatcgagt tcgccggcca ccagcagggt gggcacctgg
     8821 atgtgcgaca ggttggcgcg ccggtcgaac gtgaccaggg cctcgagcgc gcgccggtag
     8881 gtggacgcgg gaatctggct catgcaatgc gtggccagcc gcaccccttc cggcagcgat
     8941 gccggtccca ccatctgcgg caccagcacg tccgcgaggt ccgccatgct cttgccggca
     9001 tccagcggtg cggtgcgctc ggcgatgaac ttgcgctgcc agtttccttc ggccttgccg
     9061 aaggagggcg acgtcccgca caggatgagc ctgctcacca gctcggggcg gcgcgccacc
     9121 acttcctgcg ccaccatgcc gcccatgctg tggcccagaa ggatcacgcc gtctcgcctg
     9181 gcgttgtttg cagtgcccgc ggcagagccc ttttcgtctc cggccagcag cgcctcgatc
     9241 agcgtgatgc agctgcgggc cagccctttg aacgtgtacg gttcgatcgg cgcgctgcgg
     9301 ccgtaaccgg gcatgtccca ggccaccgcc cggtagcccg acgaggccag cgtttccacc
     9361 tggggcgcga aggccaaatg cccgccgccg atgccgtgca gcatgaggat ggtggggccg
     9421 ctgcccagga cggtgaagct cggaacgttc atgcggggac tttgtcattg cggaccccgg
     9481 atcgcggtcc ggggcaggcc tgatccgcaa tccacacggc gcaccatgga tcccggatcg
     9541 agtccgggat gacaaccaaa accgggatga caaccaaacc cgggatgaca acctcggtca
     9601 tcggacgacc tttcctgcat ccaccaccgc cacgccgtcg agttcgaccg tgcagttgcg
     9661 cagcggcagg tcgaagtgtc cgcgcgtgta gcgccccgcc acttcgttgg cgcccgtgct
     9721 gtagagaaaa ttgccggcga aggctcgcag ctcggtgccg ttgaagtcac gcttgtcgta
     9781 cagggccatg ctgtcccagc gcgccgccgc gttcagcccg tagccgacat ggctgaccgc
     9841 ataggcatcc cgtccggcat tgttctcgga gcccgcttcc aggtccgacc aggcagcgat
     9901 gtagctgcgc atcagctcgg catccacgcc ggcaccgtcg atcttcgtca catagtcgtt
     9961 ctcgacgagc agcgtgatcg ggcgctcgat atagcgtttg aaggtgaggt tgacgtcgcc
    10021 ttcggcaagc accagcgtgc cgttgacgct gccggccgcg ggaaacgcca acgcgagacc
    10081 gccgggccaa tgggtcatgg tgccgggccg ggtggtatag ccccagttgc cgccgcaagt
    10141 ggcgcccacc agcgagatcg cgaggtcggt ccccgcggcc gaggtcacat gcatggaccg
    10201 cgccgaacgc aggcgtttca tatggtcctt gaccagcggc tcgagcgcgt cgccgggcac
    10261 cagccgcgcc agcgcctccg gatgttcatt gctcacgtag atcacccgcg cgccgccgcg
    10321 caggatcgcc ggcagctcgc tcgcgtgcat gagtccttcc accgtgcagt ccaccacgag
    10381 cgtgcttgcg gcgagcgccg cgatgacggg tgcgagctgc tgcacggcgg tggaggcgcc
    10441 ggtcgagcgc accggcgggc ccgccgcatc gaacagcgtc ggcaccgaaa tctggaacga
    10501 ccggcagccc agacgcgccg cggccagccg cgccagctgc ggcagcaccg gacggctctg
    10561 gctttcgctg aggatggcca cggtatcgcc cggacgcagt gcacagcgcg ccagcacggc
    10621 ttcgaaggca tccacccagg cgggctctat ggtttcctgc agcatggttt cgcggttccg
    10681 gtcatactac atgaagattc atataaattg gtttatgagc ccatcgcgca aagcccagcc
    10741 gccgagcttt tccgcgcacg aattccgcaa tgcgctggga atgttcgcga ccggggtaac
    10801 gatcgttacc gcacgcactt ccgatggcag ggtcatcggc ttgacggcca actcgttcaa
    10861 ctcggtttcg ctcgatccgc cgctggtgct gtggagcttg tcgcaggccg cggcttcgct
    10921 gccggctttc cgggccggct cgcactacgc gatcaacgtg ctggccgccg agcaaaaggc
    10981 cctggccgag cgcttcgcgc tcaaggggac cgaccgcttc gccggggtga gctttgtcga
    11041 aggcaccggc ggcgccccgc tgctggccgg cgccgccgcc accttcgagt gcttcaaccg
    11101 cagccgctac gaggaaggag accacgtgat cttcgtgggc gaggtggagc gctgcagctg
    11161 gcgctcgggc gcgatgccgc tgctgttcca cggcggccag ttctataccg agcttccgat
    11221 atgaagaaag tgccggacat gggcaagacg cgcttcgtcg acagctacct gggctacctg
    11281 ctgggccagg cgaatcacgc gctgtacaag gacttcgacg cgcatgtgcg cgccgccggc
    11341 ctcagctcca tcgaatggcg ggtgctggcc acattgcacg acagcgagcc gctgaccatc
    11401 ggccagctgg cgaatgaagt gttgtccaag cagccgacgg tgaccaagct cgtgcagcgc
    11461 atggccgagc aaggatgggt ggcattgcat gccgacgcca gtgaccagcg ctgcacgcgg
    11521 gtgaccgcca ccgctgccgg caagcgcctg gtcacgccgc tggtggacga ggccaggcag
    11581 caggaagcgc gcatcctgcg gccgctggcc gccaccgaaa aggtggcgct gcgcaagctg
    11641 ctggagaaac tggcacagca cacatgagct ggcggaggtc gaacgtcgtg cccccctgct
    11701 cgtgggcgac gatgaaccgc gctaccggtt gcgcgatggc tccgctgcgc gccgatgacc
    11761 gtccgcgtta ccgcgcgagc cacggctgag caagctacca tcgatccgat gagagaacga
    11821 aaacaacatc tcgacgcact tgcgatttcg ttgctgctgg cctgctgcct gttctggggc
    11881 ttccagcaaa tcctcatcaa gaccaccgtg ggggaagtgc ccccgctgtg gcaggccgcg
    11941 ttgcggttcg tcggtgccac cgcgctgctg tggctgtggt gcgccgcgcg tggagtcaag
    12001 ctgttccagc gcgacggtac gctgcgggcg ggcctgctgg cgggtgtgct gttcgcgggt
    12061 gagttcgcct gcatctacct gggcctgcgg gacaccacgg cgtcgcggct caccgtgttc
    12121 ctctacacct cgcctttcgt ggtggcgctg ctgctgccac gcctggtgcc ctccgaaaaa
    12181 ctgcgcgtgg cgcagtggtc cggtttgctg atcgccttcg ccgcggtcgc cgtcgccttc
    12241 agtgaaggct tcagcggcgg cacgacgagc cggcagctgc gcggcgatgc cctcggattg
    12301 ggcgccggca tcctttgggg actgacaacg ctggtgatcc gcgccagcgc cctgtccacc
    12361 gccagcgcgg agaagaccct gttctaccag gtggcggtca cggcgctcgt cgcaccgctg
    12421 ctgtcgctgg cgctgggcga agcctggtcg ctcgactact cggcttatgc ctggggctcg
    12481 ctggcgctgc agacggcgat cggcgcattc gcgagctacc tcgcatggat gtggatgctg
    12541 cggcactacc cggccacgcg catgtcctcg ttcacattcc tcacgccggt gttcgcgctg
    12601 gtgctcggcg tggccttgct caaggaaccg ctgacgctgc agctgatgct ggcgctgggc
    12661 gccgtggccg tgggcatcgt gttggtcaac cgccggcccg ctgcgtgaac ttgccgtggt
    12721 ccggggcttg acccagactc gtacccatgt tgacgctcgc aatggatccc gggtcaagcc
    12781 cgggatgaca acccctgtct tgtcattgcg gacttgatcc gcaatccacg gatcccggct
    12841 caagccccgg atgacggttc attggctcgc cgtgatgccg ccgtcgacgt agagcacctg
    12901 cccggtgatg aatgacgcgg ccgggctggc gaagaaaacc gcggcaccgg ccacgtcctt
    12961 cggatcggcg acccggccca tggggatgcg gcccagcacc tgggcccggg tgtcgggatt
    13021 ttccagccag tggcgcgcca tctccgtgcg cacgactgtc ggcgcgatgc cgttcacggt
    13081 gacgccgtgc ggcgccagct cgccggcgtg ctggcggatc aacatcacga gtccgccctt
    13141 gctgccgcag tacgccgagt agccgcggcc gcgcagtccc agccgggcgc gcaccgacag
    13201 cagatggatt tgcgcgccgc cgcgcccggc cgcgatctgg tgcctggcgg ctgcctgggc
    13261 gaggaacatc gcggccttga ggttgacctg cacgacctcg tcgaacgttt cctccgtcac
    13321 ctcgagcagc gcctgctcgc gctgcatgcc cacgcagttg accaggatgt ccacgccccc
    13381 gagctcggcg gcgacacggt ccaccgagga ctggatctgc gccaccgaat gcgcatccat
    13441 ggccagcccc agcgcttcat gacccgccgc cttcaattca cccgcgagcg cttcggccct
    13501 ggccggatcg cggcccgaca cggcgacctt ggcgcccgcc agcgccagtg cccaggccat
    13561 ggcctcgccg atgccgccat agccgccggg gaggtatgcg gtcttgcctt tcaggctgaa
    13621 aaagcccgcg agctcctgcg aaaagtcgat gcgcggcgtg atgtcgctca tgcttagtcc
    13681 atgatcaggc cgccgttgag atcgacgatc tcgccggtaa tgaagccggc cagcggcgac
    13741 gccaggaaac gcaccacatg cgcgaattcc tcgggctcgc agaagcgccc gaccggtatg
    13801 tccttgagca gcctctggcg ttggtcttca ttgagctgct cggtgatcat gggcgtcttc
    13861 acataggccg gcgagatgcc gttggacgtg acgccatagg gcgccagctc gcgcgccagt
    13921 gaaaacgtca gcgaggtgag cgcgcctttc gacaccgaat aggcggtgcc cgccgtcagc
    13981 ccgccggtct tggccgccag cgagcaggtg ttgatgatgc gcccgaaccg gcgcgccttc
    14041 atggcaggca tgacgcgctg cgccagaaaa aaggcgccgt cgagattggc gccgagcacg
    14101 cgccgccact cggccgtatc ggtggcctcg gccttgttgt tggacaggat gccggcgttg
    14161 ttgaggagga tgtccgcttc gcccagcgtg gcgacgatcc gctcatggcc ctcgcgcacc
    14221 gccttcgggt cgctgatgtc gaagcagaac gcccgcgcgt tggcgcccag ttgcgccgcc
    14281 agttcctcgt tgcgctggcc attggcgtcc accatcgcga ccttgcagcc gtcctcgagc
    14341 agaaatttcg tggcggccag ccccatggtg cccgcggcac ctgtgacgac cgcaatcctg
    14401 ccttccagtg ccctcattca gcagctcctg tattcaccgg tacctctctc cgaacgcaac
    14461 ggcgcgaaaa gcgggaaaca ccgcggaacc ggcttcgccg ggccgctggt ggcgccccct
    14521 gagggggaga gggcgaagcc cgagggggca tcggccgcag gccgcttcgg gggggagcca
    14581 ttttcaggca tcgagcacgc tcttctttcc gaacaggccg cgggaacgga aggtcaccac
    14641 ggcgatgaac aacaggtaca tggacatcag cgaccactcg gaagcatacg ctccggtgag
    14701 tcccaccacc atgcccacca tcagcccggc gatcacggcg ccccacaacg agccgacgcc
    14761 ccccagcacg atgatgagga aggccggcac caccgcgtcc acgcccacgt gcggccggat
    14821 gccccagatc ggcgccatca ccactccggc gatcgccgcc agcgcgacgc ccaggccgaa
    14881 caccaatagc cgcagccgcg acaggttgat gcccagggcc cgcaccattt cgctgtcgtg
    14941 cgcccccgcc ttgatgatcg cgccgtaagg cgtcttctcg aggaacagcc acagcgccgc
    15001 gatcgcgagg atggcaaagc cgctggcaaa gaaccggtac ttcgaataga tcaggtcgcc
    15061 cagcaggaat gcgcccgaga tcgcttgcgg caaggctagc tgcttttcgg tcgagcccca
    15121 gacgacgcgg atcgtctcct cgatcaccag tgccgcaccg aaagtcagca gcaggccgta
    15181 caaggggtcg cgtccatagg tgcgccgcag gcataactcg agcagcatgc cgaacaggcc
    15241 gacgaacacc ggcgccagga acagggcaat caggtagcgg gtgtcgatcg gcagcgccat
    15301 gtaccaggcc tgcaattcgg gaaaccagcc cagccgcggc gagacgaaga acaaggcgaa
    15361 gtacatcccc agcgcgaaca gcgagccgtg ggccaggttg atcgtctcca tcacgcccac
    15421 gatgagcatg aagcccaggg cgatcagtgc gaacaacagg cccagcgaga ggccgttgag
    15481 caggtgtgga agcatgggtg ctggtcgttg agcgttaagc gttgagcgtc tggaggattt
    15541 ggggcggcgc cgaaaataac tgtcattgcg gaccccggat caagtccggg gcatgcttga
    15601 tccgcaatcc atggatcccg ggtcaagccc gggatgacag tctcacgccc aacgccgaac
    15661 gctcaatctc ttcacacttc gtagctcggc gtggcttcgt agctttccag cttgcaggcg
    15721 ttgggcgcgt ccttgtcctg cacatccttg ggatcgactt cggtcaggat cttgaagatg
    15781 tcgtccttct ctgcgggctt gtcgttgtag gccgccatgt agatggtctg ctggcagtgg
    15841 tgcgtcaccg ggtcgatcca ggcgtcgtgg tgctgcaggc ggtcggcggc ggtcatcttg
    15901 cgcccttcga gcttcttgat cacggcaagg ttgttggtgg tgccggcctc ctccacacag
    15961 cgcagcagtt cgcgcgtggc catgtagccg ttgtgataaa cgttgccggg cacgcggatc
    16021 gccatgccgg ggaactgctt ctggtacttc gccacgaact ccttcacgcc ggggagttgc
    16081 aggcgccagt accaggtggt gccgaacacg ccgaagatct gctccgggcc caggccgtag
    16141 acgtcgggcc agtcctgctg gttgttgatc caggccatgt tctggtggag cttgagctgc
    16201 accacctgct ggcgcagcgc cttgatgtcg tcgccaccga ccgcggtggc caccacctgc
    16261 ggcttcatct gctgcagctt gagcaggtag gccgagaagt cgcgcgtgtt ctgcggcacc
    16321 atcagctcgt cgatgatctt gccgccgttg gcttcgacca cggcgcgggt ggccttggac
    16381 gtgttgtggc cccagacata gtcgtt
//
LOCUS       DASWIY010000414         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3589402, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000414 DASWIY010000000
VERSION     DASWIY010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3589402"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            767..>1713
                     /locus_tag="VGA59_14645"
     CDS             767..>1713
                     /locus_tag="VGA59_14645"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX9720956.1"
                     /translation="MNPTAFRVLGQLDLRQNGLNGVEGSELTSPSAIALDARGGELHL
                     YVADTGNHRVLGWRDANSYQAGAQADIVLGQPNLRTSVPLAIGNGGLNAPLGVAVHPQ
                     TGNVYVADTDDHRVLRFPSPFENPGRVEPDRFYGQPTLASRSANAGGLSERSMNGPTT
                     VAFDRAGNLWVADRGNHRVLRIPAAALDTSETAADLVLGQPSFSSAAANGGVGAVGPT
                     GFNTPLGLAFDGAGALYVSDFTNARVLVFNAPQTTAQPANRVLGQLTFESSGAPSTPT
                     ASTLRGPVGLEVSAAGDLYVSIPLDHRVVVFNDVAQASAG"
BASE COUNT          274 a          613 c          534 g          292 t
ORIGIN      
        1 ccggcaagag ccgggtcccc ccggcgttct cctccagatc ggccaatcgc cggccccgca
       61 gaccggcgaa gcttgacccc ttgtcccccg cgccgccccg gcccctgaat ggctggcctt
      121 tggacgcggc agaatccccc cgatcaggcg atcccgcgct ggaactaggg gaaatcttcc
      181 ggccgcaggc ggtccaggct ggtgggacgg ccccaagacc gtgataaaat tggctttggc
      241 agccggctcc aagggccgag ggatccggta cttccgagaa gggctgagtt ccccgctggc
      301 gggtcttgag agtactggaa agggatccag gacctcaagt ccggtgtctg ttcccaaatc
      361 tggtgttcgg ggtaggcgta accgtggctg cggggttcgg ttcctcgaca aggcaacccc
      421 ggcgccgcag cgccgaatgc ccaccgttcc caggcattta cgccgctggg cagccgcaat
      481 ttccactgcc atccactcaa atggccatcc tcgtgcaggt agcgatcgaa tcaaaacgat
      541 gcccttgctt atgaactgta caatttcgaa cctggcgaga tcgcgtgccg cgagtccatc
      601 cacacagctc agccgctccg gacacctcag cgtccgcccc tcggtcgcaa gtcccgttag
      661 tcggagtgcc gggcgctcag gcagatgcgc aaacagacgc tggccggacc cattcgcggc
      721 cgcgctggcg ctggcgctgt ttctgtcgcc cgcgatcgcg cagcagttga accccaccgc
      781 ctttcgcgtg ctcggccagc tggacctgcg ccagaacggc ttgaacggcg ttgagggctc
      841 ggaattgact tcgccttcgg cgatcgcctt ggacgcgcgc ggcggcgagt tgcacctcta
      901 cgtcgctgat acgggcaacc atcgcgtgct gggctggcgc gacgcgaaca gctatcaggc
      961 aggtgcccag gccgatatcg tactcggcca gccgaacctg cgcacctcgg tacccctggc
     1021 gatcggcaac ggcgggctca acgcgccgct cggcgtggcg gtccatcccc agaccggcaa
     1081 cgtctacgtg gcggacaccg acgaccaccg cgtgcttcgc tttcccagtc cgtttgaaaa
     1141 tcccggccgc gtggagcccg accgtttcta cgggcagccc acgctcgctt cgcgcagcgc
     1201 gaatgccggc ggcttgagcg agcgatcgat gaacggcccc acgacggtgg ctttcgaccg
     1261 cgcgggtaac ctgtgggtcg ccgaccgcgg caaccaccgc gtcttgcgga tacccgccgc
     1321 cgcgctcgat acgagcgaga cggcggcgga tctggttctg ggacagccga gtttctcttc
     1381 cgctgccgcc aacggcggag tcggcgccgt cggcccaacg ggctttaaca cgccgctcgg
     1441 cctcgccttc gatggcgccg gcgcgctcta cgtttccgat ttcaccaacg cccgcgttct
     1501 tgtcttcaac gcccctcaga ccaccgctca gccagccaac cgcgtgctcg gacagctcac
     1561 gttcgagtcc agcggcgcgc cgtcgacgcc aacggcttcg acgctgcgcg gaccggtggg
     1621 ccttgaggtg agcgctgccg gcgacctgta cgtcagcatc ccattggacc accgcgtggt
     1681 ggtcttcaac gacgtcgccc aggcctccgc cgg
//
LOCUS       DASWIY010000415         8107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3590123, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000415 DASWIY010000000
VERSION     DASWIY010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3590123"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1356)
                     /locus_tag="VGA59_14650"
     CDS             complement(130..1356)
                     /locus_tag="VGA59_14650"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9720957.1"
                     /translation="MRAARRGVRPSPRWGDLARTETRILSTFLMLPARAFAAAAVLPS
                     PLISFFNDPRRFKTMKTTLRTLALAASLACLATTAMAQETVRWGVPMAFGSNLVALGD
                     TMPWVSEQLKKVSGGTINLQVFEPGKLVPALAIFDSVSSGKVEAGYSWMGYELGKVPV
                     SALFGAVPFGMETNMFSAWIYFGGGDALLKEAFKPHNVYPIFCGSISPEAAGWFRKEI
                     NTPDDLKGLKFRAAGYGGKVYQKLGASVTMLPAGELFQALEKGVLDGTEFSLPTVDEQ
                     LGFSKVAKNYYMPGWHQPSTNQFLYVNMAAWNKLKPQTKAQIETTCTAGVTMAIAKAE
                     ALQGATLAKFEKDGVKARQFNKVMLDAFAKATKEVMAEESAKDPLFKKVFDSMNAFQQ
                     KNQAWHHLGYLPRDHK"
     gene            complement(1569..1991)
                     /locus_tag="VGA59_14655"
     CDS             complement(1569..1991)
                     /locus_tag="VGA59_14655"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9720958.1"
                     /translation="MDELGITYRGVVYPWHCDHVGHMNVMWYVGKFDEATWNFFHHVG
                     IIASYLRENQRGMAAVDQHIEYRRELRAGDVVTVRSQLLELHEKKIRFRHEMRNDGTG
                     EVAAVTTLLGLHMDTLARKSCPFPPELLERARALLTTV"
     gene            complement(2005..5379)
                     /gene="icmF"
                     /locus_tag="VGA59_14660"
     CDS             complement(2005..5379)
                     /gene="icmF"
                     /locus_tag="VGA59_14660"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HEX9720959.1"
                     /translation="MTDLSAEFKALANYRPTHKVRFVTAASLFDGHDAAINIMRRILQ
                     GMGAEVIHLGHNRSVDEVVTAALQEDVQGIAVSSYQGGHLEYFKYMVDLLKSRGGEHI
                     QVFGGGGGVIVPPEIRELQAYGVARIFSPEDGQRMGLAGMIGEMVMRCDKDLAAYAPK
                     DVKAIQGHGELNWRALAQLITALETGKADAKLMQAVKSAAAARPIPVVGITGTGGSGK
                     SSLTDELVRRLRLDQGDSLRIAVISIDPSRRKTGGALLGDRIRMNAIGPWSSSAPTLA
                     AEAAALPPGGAVPVLGRPGDGGSRVFMRSLATRDFGSEISAALPDVISATKAAGFDLI
                     VVETSGIGQGDAAIVPLVDIPMYVMTPEFGAASQLEKIDMLDFAEFVAINKFDRKGSL
                     DALRDVAKQVQRNKEAFGRKPDEMPVFGTMAARFNDDGVTALYQALKPRLAELGLKLK
                     DGILPAVTVRHSTNQTPVIPASRTRYLAEISDTVRGYKKLAREQAKLAREIQQLRASA
                     SMLRLCDGNIATAADAAEGLAAQREETQDPGARKLLAQWPQMRSAYAGDEYVVRIRDK
                     ELRTALTSKSLSGTTIRKVCLPAFEDHGEILKWLMLDNVPGSFPYTAGTFAFKRENED
                     PTRMFAGEGDAFRTNTRFKLLSSGMQAKRLSTAFDSVTLYGNDPDLRPDIYGKVGNSG
                     VSIATLDDMKVLYSGFDLCSPNTSVSMTINGPAPTILAMFMNTAIDQNLEKFKADNGR
                     DPTDTESAKIREWVLANVRGTVQADILKEDQGQNTCIFSTEFSLKVMGDIAEYFVHHN
                     VRNFYSVSISGYHIAEAGANPISQLAFTLSNGFTLVEAYLARGMHIDDFAPNLSFFFS
                     NGMDPEYTVMGRVARRIWAVAMKEKYGANERSQKLKYHIQTSGRSLHAQEIQFNDIRT
                     TLQALIAIYDNCNSLHTNAFDEAITTPTEDSVRRAMAIQLIINREWGLAKNENPNQGA
                     FIIEELTELLEEAVLAEFERIAERGGVLGAMETGYQRGRIQDESLHYEMLKHTGELPI
                     VGVNTFRNPHGDAVHDKLELARSTEEEKQSQLKRLQEFQARNAKQAPAMLKRLQQAVI
                     DNRNVFEVLMDAVRYCSLGQITNALFEVGGQYRRNM"
     gene            5712..6875
                     /locus_tag="VGA59_14665"
     CDS             5712..6875
                     /locus_tag="VGA59_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9720960.1"
                     /translation="MNESTQIADGAPTAAKPARRRFLRAAVGGVAGAAAMTAPMVSVA
                     QSPVVLKMQGAWGAKDIFNDMAVEYVTRVNEMAGGRLRIDYLLAGAVVKPFEVMDATS
                     KGVLDASHGVPVYWYGKSKVASLFGSGPITGCDAPQTLAWIYRGGGLELYNELLKKIN
                     LDVVGYFAMPMPTQPLGWFKKPIKSAAELKGLKYRTVGLAADLFQEMGTKVTQLAGGE
                     IMPAMDRGVIEAFEFNNPTSDSRFGAQDVAKNYMMGSFHQAMEFFEIAFNKKKHDSLP
                     KDLQAILRYAAEAASSSNWWTAMDNYSRDLEELASKHKVNVLRTPKPIMEAQLKAWDV
                     ITKRLSDEDPFFKKVADSQRAWAKRVAKYMFLNEADYLAGYQHIHGKIPGLSA"
     gene            6950..7519
                     /locus_tag="VGA59_14670"
     CDS             6950..7519
                     /locus_tag="VGA59_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9720961.1"
                     /translation="MIASFIKSIDLLSKSVGHAFGWCIVILILGVSYEVFMRYALNDP
                     TSWAFDFSFILYGALFIMAGPYALSRNGHVRGDIFFRTWRPRVQAGLELFLYFIFFFP
                     GVLALVIAGWFYGIEALRIREVSVNSPIGVPIWPLKMLIPVAGTLLTLQGIAEVLRCL
                     LCLRTGAWPPRLHDVEELEKQLVSSQAST"
     gene            7519..>8107
                     /locus_tag="VGA59_14675"
     CDS             7519..>8107
                     /locus_tag="VGA59_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9720962.1"
                     /translation="MSNPQIALLMLGSFVFVILLGFPIAFTLMAMGVMFGYYAYFQDG
                     QAFFDNRVFYLFTQNTFSVMNSDILVAVPLFLFMGYMIERSNILDKLFLSLQIAFRRV
                     PGAMAIAALVTCALFATATGIVGAVVTLMGLLAFPAMLKANYDQKISAGVICAGGTLG
                     ILIPPSIMLIVYGATAGVSVVRLYAAALLPGFFLAG"
BASE COUNT         1368 a         2518 c         2551 g         1670 t
ORIGIN      
        1 tcagctcttc gacctcatgc aaatgcgatt ccatcactct tctcctggcg caaaaacgag
       61 ccgttccggg aagctcgggc tccccggaac ggcttgagag acgcttcgta ttgtcgtgcg
      121 gttcagcgat tacttatggt cacgcggcag gtagccgagg tggtgccaag cctgattctt
      181 ttgctggaac gcgttcatgc tatcgaaaac tttcttgaac agagggtctt tggccgactc
      241 ttcagccatg acttccttcg tggccttggc gaatgcatcc agcatgactt tattgaactg
      301 acgggccttg acgccgtcct tttcgaactt cgcgagggtt gcgccctgca gcgcctcggc
      361 cttcgcgata gccatcgtca cgccggcggt gcaggtggtt tcgatctgcg ccttggtttg
      421 cggcttcagc ttgttccagg cagccatgtt cacgtacagg aactggttcg tggacggttg
      481 atgccagccg ggcatgtagt aattcttggc aaccttggag aagccgagct gttcatccac
      541 cgttggcagc gaaaactcgg tgccgtcgag cacgcccttt tccagcgcct ggaacagttc
      601 gccggcaggc agcatggtca ccgaagcgcc cagcttttga tagaccttgc cgccatagcc
      661 ggcggcgcgg aacttcaggc ccttcaggtc atccggcgta ttgatctcct tgcggaacca
      721 gccggccgct tccgggctga tcgagccgca gaagatcgga tagacgttgt ggggcttgaa
      781 ggcttccttg agcaatgcgt caccgccgcc gaagtaaatc cacgctgaaa acatgttggt
      841 ttccatgccg aaaggcaccg cgccgaacaa ggcggagacc ggcaccttgc cgagttcgta
      901 gcccatccag ctatagcctg cctccacctt gccgctcgac acgctgtcga aaatcgccag
      961 cgccggcacc agcttgcccg gctcgaacac ttgcaggttg atcgttccgc cggacacttt
     1021 cttcagttgc tcggagaccc agggcatggt gtcgcccagt gcgaccaggt tcgagccgaa
     1081 ggccatcggg acaccccaac ggaccgtttc ctgggccatc gcggtggtgg caaggcaggc
     1141 aagcgaagct gccaatgcga gagttcgtag cgtggtcttc atcgtcttaa atctccgtgg
     1201 gtcgttgaaa aatgaaatga gtggcgaggg aaggacggcg gccgccgcaa aagcacgggc
     1261 gggcagcata agaaatgtgg aaaggatgcg cgtttctgtt ctggctaggt ctccccacct
     1321 cggtgacggt cgaacgcctc ttcttgctgc ccgcaatggt agggaccttg gtttgagctt
     1381 ggatcagtag ttaccctttt atgcttcaca agcgggcttt ccgcaaccag tcgcgtgcca
     1441 ggttcttgtt gagctgaact ctcgcatgac ctgccgcgtg tgaggtggca aggcttcgtt
     1501 gctacaaatt tcatagctaa cccgggattc gcggcaggct gtcagaagcc tgactggtgc
     1561 gatgacgtct agaccgtcgt gagtagcgcg cgggcacgtt ccagcagctc gggcggaaac
     1621 gggcaggact tgcgggcgag ggtgtccatg tgcagcccga gcagcgtggt gacggccgcc
     1681 acttcaccgg tgccgtcgtt cctcatctcg tggcggaagc ggattttctt ctcgtgcagc
     1741 tcgagcagct gcgagcgcac ggtcaccacg tcgccggccc tgagttcgcg cctgtattcg
     1801 atgtgctggt cgacggccgc catgccccgc tggttctcgc gcaggtagct cgcgatgatg
     1861 cccacatgat gaaagaaatt ccaggtagcc tcgtcgaact tgcccacgta ccacatgaca
     1921 ttcatgtgcc ccacatggtc gcaatgccag ggatagacaa cgccgcggta ggtgatccca
     1981 agctcgtcca tggttagtgt cggcctacat gttccggcgg tactgcccgc cgacctcgaa
     2041 cagcgcgttc gtgatctgcc ccagcgagca gtagcgcacc gcgtccatca ggacttcgaa
     2101 cacgttgcgg ttgtcgatca ccgcctgctg cagccgcttg agcatggccg gagcctgctt
     2161 cgcgttgcgc gcctggaact cctgcaggcg cttgagctgg ctttgcttct cctcttcggt
     2221 cgagcgcgcc agttcgagct tgtcatgcac cgcgtcgccg tgcggattgc ggaaggtgtt
     2281 gacgccgacg atcggcagct cgccggtgtg cttgagcatc tcgtagtgca gggattcgtc
     2341 ctggatgcgc ccgcgctggt agccggtttc catcgcgccg agcacgccgc cgcgctccgc
     2401 gatgcgctcg aattcggcga gcacggcttc ttccagcagt tcggtcagct cttcgatgat
     2461 gaacgcgccc tggttggggt tctcgttctt cgccaggccc cattcgcggt tgatgatcag
     2521 ctggatcgcc atcgctcgcc gcacgctgtc ctcggtgggc gtggtgatcg cctcgtcgaa
     2581 cgcgttcgtg tgcagcgaat tgcagttgtc gtagatcgcg atcagcgcct gcagcgtggt
     2641 gcggatgtcg ttgaactgga tctcctgcgc gtgcaggctg cgccccgagg tctggatgtg
     2701 gtacttgagc ttctggctgc gctcgttggc gccgtacttc tctttcatgg ccacggccca
     2761 gatacggcgt gccacgcggc ccatcaccgt gtactccggg tccatgccgt tggagaagaa
     2821 gaacgacagg ttgggcgcga agtcgtcgat gtgcatgccc cgggcgagat acgcttccac
     2881 cagggtgaag ccgttcgaga gcgtgaaggc cagctggctg atcgggttgg cgccggcctc
     2941 ggcgatgtgg tagcccgaga tcgacaccga gtagaagttg cgcacgttgt ggtgcacgaa
     3001 gtactcggcg atgtcgccca tcaccttcag gctgaactcg gtggagaaga tgcaggtgtt
     3061 ctggccctgg tcctctttca ggatgtcggc ttgcacggta ccgcgcacgt tggccagcac
     3121 ccattcgcgg atcttggcgc tttccgtgtc ggtcgggtcg cgcccgttgt cggccttgaa
     3181 cttctccagg ttctgatcga tggcggtgtt catgaacatc gccaggatgg tgggcgccgg
     3241 gccgttgatg gtcatggaca ccgaggtgtt cgggctgcac aggtcgaatc ccgaatacag
     3301 caccttcatg tcatccagtg tggcgatcga cacgccggag ttgcccacct tgccgtagat
     3361 gtcggggcgc aagtcggggt cgttgccgta cagggtcacg gaatcgaatg cggtggacag
     3421 gcgcttggct tgcatgcccg aactcagcaa cttgaaccgg gtgttcgtgc ggaaggcgtc
     3481 gccttcgccg gcgaacatgc gcgtcgggtc ctcgttctcg cgcttgaaag cgaacgtgcc
     3541 ggccgtgtaa ggaaagcttc ccggtacgtt gtccagcatc agccacttga ggatttcgcc
     3601 atggtcctcg aacgcaggca ggcagacctt gcggatggtg gtacccgaca gcgacttgct
     3661 ggtgagcgcc gttcgcagtt ccttgtcgcg gatcctgacc acgtactcgt cgccggcata
     3721 ggccgagcgc atctgcggcc actgcgccag cagcttgcgc gcgcctgggt cctgcgtctc
     3781 ttcgcgctgg gcggccagcc cttccgcggc gtcggcggca gtcgcgatgt tgccgtcgca
     3841 caagcgcagc atcgaggccg aggcgcgcaa ctgctggatc tcacgggcca gctttgcctg
     3901 ctcgcgggcc aacttcttgt agccgcgcac cgtgtcgctg atctcggcga ggtaacgcgt
     3961 gcgtgacgcc gggatcaccg gcgtttggtt cgtgctgtgg cgcacggtga cagccgggag
     4021 gatgccgtcc ttgagcttca ggcccagttc cgccaggcgg ggtttgagcg cctggtacag
     4081 cgccgtgacc ccatcgtcgt tgaagcgagc cgccatcgtg ccgaacaccg gcatttcgtc
     4141 cggcttccgg ccgaaggctt ccttgttgcg ctgcacctgc ttggcgacgt cgcgcagtgc
     4201 gtcgagcgag cccttgcggt cgaacttgtt gatcgccacg aactcggcga agtcgagcat
     4261 gtcgattttt tccagctggc tcgccgcgcc gaattccggc gtcatcacgt acatcgggat
     4321 gtccaccagg ggcacgatcg ccgcgtcgcc ctggccgatt ccggaagtct ccacgacgat
     4381 caggtcgaag ccggctgcct tggtggccga gatgacatcg ggcagggcgg cggaaatctc
     4441 actgccgaag tcccgggtgg ccagggagcg catgaaaacc cgggatcccc cgtcgccggg
     4501 ccgccccaag acggggacgg cccccccggg gggcagcgca gcggcttcag ccgcaagcgt
     4561 gggggcactt gagctccagg gaccgatcgc gttcatgcgg atgcgatcgc ccagcagggc
     4621 gccgcccgtc ttgcggcgcg agggatcgat cgagatcacc gcgattcgca gactgtcgcc
     4681 ctggtccagg cgcaacctgc ggaccagctc gtcggtcagc gaggacttgc ccgacccgcc
     4741 ggtgccggtg atgccaacca ccgggatggg gcgcgccgcc gcggcgctct tcaccgcctg
     4801 catgagcttg gcgtcggcct tgccggtttc cagggccgtg atcagctggg ccagggcccg
     4861 ccagttcagc tcgccatggc cctggatggc cttgacgtcc ttgggcgcat acgctgccag
     4921 gtccttgtcg cagcgcatga ccatctcgcc gatcatgccg gccagcccca tgcgctggcc
     4981 atcctccggg ctgaagatgc gagcgacgcc ataggcctgc agctcgcgga tctcgggagg
     5041 cacgatgacg ccgccgccgc cgccgaacac ctggatgtgc tcgccgccgc gggacttgag
     5101 caaatccacc atgtacttga agtattccag atggccaccc tggtaggagc tgacggcgat
     5161 gccctgcacg tcctcctgga gcgcggcggt gacgacctcg tccacgctgc ggttgtgtcc
     5221 caggtggatc acttcggcgc ccatgccttg caggatgcgc cgcatgatgt tgatcgccgc
     5281 gtcgtggccg tcgaacaggc tggcagccgt gacgaaccgg actttgtggg tggggcggta
     5341 attggcgagc gccttgaatt cggcggacag gtcggtcatg gaggtctccg ccgggacgca
     5401 cttgccccgg atgcagcaat ggaattcccg taccttactt gacgtttacg taaacgtcaa
     5461 ctgtgccgga caccacactc gttcccggcg ggatcgcgcg atttgtaata cggtggttag
     5521 cacgctatct ttatgcgtgc aacccaggcg gcgccagcgc ccttcatttg gggtaatccc
     5581 ccagagacag aaagggtcaa gctcgtaacg tagcggggtt cgtaacggaa ggagttttcg
     5641 ttaacgggac gcaacctttg cgggcgcttt tatgcgtaca tttttagatc gacattccaa
     5701 ggagacaagt catgaatgag agtacccaga tcgctgacgg cgctcccact gccgccaagc
     5761 cggctcgccg ccgcttcctc cgggccgccg ttggcggcgt ggctggcgct gccgcgatga
     5821 ccgcacccat ggtttccgtg gcccagtcgc cagtcgtcct gaagatgcag ggtgcctggg
     5881 gcgccaagga catcttcaac gacatggcgg tggagtacgt cactcgcgtc aacgagatgg
     5941 ccggcggccg cctgcgtatc gactacctcc ttgcgggcgc cgtcgtgaag cccttcgagg
     6001 tgatggacgc cacgagcaaa ggcgtgctcg acgcttcgca cggcgtgccg gtttactggt
     6061 acggcaagag caaggtagcc tcgctgttcg gttcgggccc catcaccggc tgcgacgccc
     6121 cccagacgct cgcctggatc taccgtggcg gcgggctgga gctgtacaac gagcttctca
     6181 agaagatcaa cctcgacgtg gtgggctact tcgcgatgcc gatgccgacg cagcccctgg
     6241 gctggttcaa gaagccgatc aagtcggccg ctgagctcaa gggcctgaaa taccgtaccg
     6301 tgggcctcgc ggccgacctg ttccaggaaa tgggcacgaa agtgactcag ctggccggcg
     6361 gcgagatcat gccggcgatg gaccgtggcg tgatcgaggc cttcgagttc aacaatccca
     6421 catcggacag ccgtttcggc gcccaggacg tggccaagaa ctacatgatg ggcagctttc
     6481 accaggcgat ggaattcttc gaaatcgcct tcaacaagaa gaagcacgat tcactgccga
     6541 aggacctgca ggcgatcttg cgctatgccg ccgaagccgc ctcgtcctcg aactggtgga
     6601 ccgccatgga caattattcg cgcgacctgg aagaactcgc cagcaagcac aaggtaaacg
     6661 tgctccgcac gcccaagccg atcatggaag cgcagctcaa ggcatgggac gtgatcacca
     6721 agaggctgtc cgacgaagat ccgttcttca agaaggtggc cgacagccag cgcgcctggg
     6781 ccaagcgcgt ggccaagtac atgttcctga atgaagccga ctatctggcc ggctaccagc
     6841 acattcacgg caagattcct ggactttccg catagcgtgt tgcgtcccgc cgagccggca
     6901 acggctttcg agggaggctt cggcctccct ctcttcgtga gcaagaaaaa tgatcgcttc
     6961 cttcatcaag agcatcgacc ttctcagcaa gtcggtcggc cacgccttcg gctggtgcat
     7021 cgtgatactg atcctgggcg tgagctacga agttttcatg cgctacgcgc tcaacgatcc
     7081 gacaagctgg gcattcgact tcagcttcat cctttacggc gccctgttca tcatggcagg
     7141 gccgtacgcg ctgtcgcgca acggccacgt tcgcggcgac atcttcttcc gcacctggcg
     7201 gccccgggtg caggccggtc tggagctgtt cctgtatttc atcttcttct ttcccggtgt
     7261 gctggcgctg gtgatcgccg gctggttcta cggcatcgag gccttgcgca tccgcgaggt
     7321 gagcgtcaac agcccgatag gcgtgccgat ctggccgctg aagatgttga tcccggtggc
     7381 cggcacgctg ctgacgctgc agggcatcgc cgaggtgctg cgctgcctgc tgtgtctgcg
     7441 caccggcgcc tggccgcctc ggctgcatga cgtcgaggag ctcgaaaagc agctggtgtc
     7501 cagccaggcg agtacctgat gagcaatccc cagatcgccc tgctgatgct gggctccttc
     7561 gttttcgtca ttctcctggg ctttccgatt gccttcacgc tgatggcgat gggtgtgatg
     7621 ttcggctact acgcctactt ccaggacggc caggccttct tcgacaaccg cgttttctac
     7681 ctgttcaccc agaacacctt cagcgtgatg aacagcgaca tcctggtggc ggtgccgctg
     7741 ttcctgttca tgggctacat gatcgagcga tcgaacatcc tggacaagct gttcctgagt
     7801 ttgcagatcg ccttccggcg cgtaccggga gcgatggcca tcgcggcgct ggtcacctgc
     7861 gccctgttcg ccaccgccac gggcatcgtg ggggcagtgg tcaccctgat gggcctgttg
     7921 gcatttcccg cgatgctcaa ggccaactac gaccagaaaa tctccgccgg cgtcatctgc
     7981 gccggcggga cgctcggcat cctgattccg ccctcgatca tgctgatcgt gtatggagcc
     8041 acggccggcg tttcggtggt caggttgtac gccgccgcct tgctgccggg cttctttctg
     8101 gcaggcc
//
LOCUS       DASWIY010000416        11129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2516368, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000416 DASWIY010000000
VERSION     DASWIY010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2516368"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VGA59_14680"
     CDS             complement(<1..188)
                     /locus_tag="VGA59_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720963.1"
                     /translation="MLPAISRGGGVDFSLWVSLEKDRTARRWEACGDHGREPQSLKDG
                     LEHCAVHNPLHDKELPDV"
     gene            98..1417
                     /locus_tag="VGA59_14685"
     CDS             98..1417
                     /locus_tag="VGA59_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9720964.1"
                     /translation="MPPICEQYGLFQGKPTVKNQRRRLLKSLAASLLVPAAAWAQGPL
                     PPVKLAMIEAMSGAFANTGEAVFRNLVWAVERVNARGGVRLPGGNRLLALERYDSKGQ
                     NEEALSALRSAIDDGAQYILQGNSSAVAAALIDAVNKHNERDPGKRVLFLNYSAVDPI
                     LTNEKCSFWHFRFDAHADMRMAALMEVLKEDRGVASAYLIGQDYSFGQAVLREARRQL
                     GAQRPDIRIVGDELHPMGRVKDFLPYAAKIKATGAQAVITGNWGNDLTLLVRAARDVG
                     FEGKFYTFYGNALGAPAAIGDAGIGRIVAVADWLPNVATAPSEAFYRSFRRRYPKPAD
                     DYVHMRMQLMIEALAQAIEKAGSLDAVAVASALEKASVDLHGQQGFVRALDHQFQQQL
                     VVGVMDRQGAPGVKFDVEGSGYGFRVVKTITPAQAGLPTSCKMVRPG"
     gene            1393..2607
                     /locus_tag="VGA59_14690"
     CDS             1393..2607
                     /locus_tag="VGA59_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9720965.1"
                     /translation="MQDGPSRLDSGTSPKVFAMRQAIQNLEESKIREVANAGMGRSDV
                     LAFWFGEGDEVTPDFIRQAAIESLRSGETFYAHNLGLPELREAVARYTSGLHGQVAAN
                     RIAVTSGGVNALMLAVQALVDAGDEVVAVTPVWPNLTAQPLIMGARLKRVSLKPDAGA
                     WTLDLQALLDAVTADTRLLVINAPNNPTGWTLTRAEQQAILDHCRKTGTWILADEVYE
                     RLFFEPSPNGCAPSFLDIASPDDRLVVVHSFSKSFLMTGWRLGWLIMPAAMTPHMGKL
                     VEFNTSCASVFTQRAGLVALERTDEVTPRVVAHLKTCRDTLVPLLQALPRVELEAAKG
                     GMYAFFKLDGFGDSLALAKRLVAEAGLGLAPGNAFAPEAQGWLRWCFASKDVSRLERG
                     VERLSGWLAARK"
     gene            2791..3054
                     /gene="rpsO"
                     /locus_tag="VGA59_14695"
     CDS             2791..3054
                     /gene="rpsO"
                     /locus_tag="VGA59_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX9720966.1"
                     /translation="MTALNKAEVVKANARSANDTGSPEVQVALLTARINELTPHFKTH
                     AKDHHGRRGLLRMVNRRKSLLAYLKDKDAERYTALIGKLGLRK"
     gene            3316..5649
                     /gene="pnp"
                     /locus_tag="VGA59_14700"
     CDS             3316..5649
                     /gene="pnp"
                     /locus_tag="VGA59_14700"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX9720967.1"
                     /translation="MSIFNKVTKSFQWGSHTVTLETGEIARQSGGAVIVNIDDTVVLA
                     TVVASKGAKPGQDFFPLTVDYIEKTYAAGKIPGSFFKREAKPSEHETLTSRLIDRPIR
                     PLFPEGFFNEVHVVIHTLSLNPEVDADIAAMIGTSAALAISGIPFAGPIGAARVGYVD
                     GEYVLNPGQTARKLSDLDLIVAGTESAVLMVESEAQQLSEEVMLGAVVFGHEQGKIAI
                     NAIHELVRDAGKPVWDWQPPARDEAFIAKVNALAEEKLRAAYQIRSKQARTQALREAG
                     GAVQADLKAEGFEFEAAKLDALMFDIEAKIVRSQILAGEPRIDGRDTRTVRPIEIRTG
                     ALPRTHGSALFTRGETQALVVTTLGTERDAQRIDALMGEYEDRFMFHYNMPPFATGEA
                     GRMGTTKRREIGHGRLAKRALLACLPTKDEFPYTMRVVSEITESNGSSSMASVCGGCL
                     SLMDAGVPMKAHVAGIAMGLIKEGNRFAVLTDILGDEDHLGDMDFKVAGTTNGVTALQ
                     MDIKIQGITREIMQVALAQAKEARMHILGKMQETMAEAKTEVSNYAPRLFTMKINPEK
                     IRDVIGKGGSVIRALTDEFGCQINIEEDGTITIASTDPSRAEDARKRIEQITAEVEIG
                     KVYEGPITKLLDFGALVNLLPGKDGLLHISQIAHERVEKVTDYLSEGQIVRVKVLETD
                     EKGRVKLSMKALLDRPAMPPRDEQPRGDRQPRGDQPPRDQQFREQRDQPPRDQQFREQ
                     RDQPPRDQQFREQRDQQQREPREERDVPQQTPVDSQE"
     gene            5764..6762
                     /locus_tag="VGA59_14705"
     CDS             5764..6762
                     /locus_tag="VGA59_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX9720968.1"
                     /translation="MKAVEISAYGKPEVLRLGERPAPVPAAGELLIRVSASGINRPDV
                     LQRTGNYPVPPGASDIPGLEVAGEIVSGDENALAQAGFKVGDRVCALVAGGGYAQLCV
                     APVGQCLPVPQGLTDVEAASLPETFFTVWSNVFDRVKLQPGETLLIQGGTSGIGVTAI
                     QMAKALGAKVIATAGSDEKCQACLKLGADHAINYRTQDFAAEAKKLSGGAGVNVILDM
                     VAGGYVAREVECLAEDGRLVIIAVQGGVKSEFNAGLVLRKRLAITGSTLRPRPVEFKA
                     AIARSLKEKVWPLLENGAIRPVIHSVFPAAQAARAHELMESNQHIGKIVLNWTEEK"
     gene            6759..7502
                     /gene="tpiA"
                     /locus_tag="VGA59_14710"
     CDS             6759..7502
                     /gene="tpiA"
                     /locus_tag="VGA59_14710"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX9720969.1"
                     /translation="MKKKLIAGNWKMNGSTAANEDLVRALIGGMGDAACTVAVCVPAP
                     YLAQVQMLRAGSRLELGAQDVSEHAEGAYTGEISAAMLKEFGVRYAIVGHSERRQYHA
                     ETDAIVADKTKAALAAGITPIVCVGETLAQREAGRTVDIVKRQMAAVIHTNGHCISEI
                     VVAYEPVWAIGTGRTATPEQAQEVHAVLRTQLKAATEHVDRIHILYGGSMNAANAASL
                     LAQPDIDGGLVGGASLKAADFLQIIAAAR"
     gene            7538..7969
                     /gene="secG"
                     /locus_tag="VGA59_14715"
     CDS             7538..7969
                     /gene="secG"
                     /locus_tag="VGA59_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX9720970.1"
                     /translation="MNNVLLTVILAVQMLAALCMVGLILIQHGKGADMGAAFGSGSSG
                     SLFGASGSANFLSRTTAVLAAVFFVCTLALAYFGNLRPVESGSVLERAATTPVLPAPA
                     PAPAASGAAAQIPGTAAPAPASTTTAPAAPAASGAGQIPVN"
     gene            8082..8166
                     /locus_tag="VGA59_14720"
     tRNA            8082..8166
                     /locus_tag="VGA59_14720"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8116..8118,aa:Leu,seq:gag)
     gene            8223..8594
                     /locus_tag="VGA59_14725"
     CDS             8223..8594
                     /locus_tag="VGA59_14725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX9720971.1"
                     /translation="MNLDQYLPVLLFLLVGAGVGVAPMVMGWMFSSVLGFQRPDAAKN
                     SPYECGFEAFDDARMKFDVRYYLVAILFILFDLEIAFLFPWAVALKEIGPLGFWAMMV
                     FLTILVVGFVYEWKKGALDWE"
     gene            8628..9161
                     /locus_tag="VGA59_14730"
     CDS             8628..9161
                     /locus_tag="VGA59_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX9720972.1"
                     /translation="MANEGILERVPDKSILDRAPDKGLLDKGLVTTTVDTVINWAKTG
                     SLWPMSFGLACCAVEMMHAGAARYDIDRFGMLFRPSPRQSDLMIVAGTLCNKMAPAMR
                     KVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRVVPVDVYVPGCPPTAEALLYG
                     IIQLQQKIRRVQTIARA"
     gene            9175..9783
                     /locus_tag="VGA59_14735"
     CDS             9175..9783
                     /locus_tag="VGA59_14735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9720973.1"
                     /translation="MTLFAVDPQALKETLATTLGALARRIDLKLGEVTVVVAPAGYLE
                     AARLLRDTPGCQFEQLVDLCGVDYSEYRGGGWEGPRYCVVSHLLSVTLNQRVRLKVFA
                     PDDELPVLDSLTGLWSSANWFEREAFDLFGVIFEGHPDLRRILTDYGFIGHPFRKDFP
                     MEGHVEMRYDAERGRVIYQPVTIEPREITPRIIREENYGGLH"
     gene            9880..>11129
                     /locus_tag="VGA59_14740"
     CDS             9880..>11129
                     /locus_tag="VGA59_14740"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX9720974.1"
                     /translation="MAEIKNYTLNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGMLH
                     RATEKLAETKTYLQTLPYMDRLDYMSMMSNEQGYCLAVEKLLGVDVPVRAQYIRVMFA
                     EITRLLNHLLWLGASGLDCGAMNVLLYAFREREDLFDVYEAVSGARMHAAYFRPGGVY
                     RDLPDTMPQYKPSKIKSERAIKRLNENRQGSVLDFIDDFTKRFPHYVDEYETLLTENR
                     IWKQRTVGIGVVSPDRALNLGFTGPMLRGSGVAWDLRKKQPYEVYDRLDFDIPIGRNG
                     DCYDRYLVRVQELRQSNRIIKQCVDWLRANPGPVITDNHKVAPPDRESMKANMEELIH
                     HFKLFSEGMRVPEGEAYAAIEHPKGEFAVYIVSDGANKPYRLKIRPPGFVHLSAMDEM
                     ARGHMIADAVAVIGTLDIVFGEIDR"
BASE COUNT         2072 a         3643 c         3619 g         1795 t
ORIGIN      
        1 aaaacatcgg gtagctcctt gtcatgaaga gggttgtgaa cggcgcagtg ttccagtccg
       61 tcctttaaac tttgcggctc tcgaccgtga tcgccgcatg cctcccatct gcgagcagta
      121 cggtcttttt caagggaaac ccacagtgaa aaatcaacgc cgccgcctct tgaaatcgct
      181 ggcagcatcg ctacttgtcc cggccgccgc gtgggcgcaa ggcccgttgc cgcccgtgaa
      241 gctcgccatg atcgaagcca tgtccggggc gttcgcgaac accggcgaag cggtgttccg
      301 caacctggtc tgggcggtgg aacgggtgaa tgcacgcggc ggcgtcaggc tcccgggcgg
      361 caaccggctg ctggcgctgg agcgctatga cagcaagggc cagaacgagg aagcgctgtc
      421 ggcgctgcga tcggccatcg acgacggtgc gcagtacatc ctgcaaggca attcgtccgc
      481 cgttgcggcg gcgctgatcg acgccgtgaa caagcacaac gagcgcgacc cgggcaagcg
      541 cgtgctgttc ctgaattact cggccgtgga ccccatcctg accaacgaga agtgcagctt
      601 ctggcatttc cgtttcgacg cccatgccga catgcgcatg gcggcgctga tggaagtgct
      661 caaggaggac cgtggcgtcg cgagcgccta cctgatcggc caggactaca gttttggcca
      721 ggccgtgctg cgcgaggccc ggcgccagct tggcgcgcag cggcccgaca tccggatcgt
      781 cggcgacgag ctgcacccca tgggccgcgt gaaggatttc ctgccttatg ccgccaagat
      841 caaggccacc ggcgcccagg ccgtgatcac cggcaattgg gggaacgacc tgacgctgct
      901 cgtcagggcc gccagggacg tgggcttcga gggcaagttc tacaccttct acggcaacgc
      961 gctgggcgcg ccggcggcga tcggcgacgc cggcatcggc cgcatcgtgg cggtggccga
     1021 ctggctgccc aacgtcgcga cggcgcccag cgaggccttc taccggagct tccgccggcg
     1081 ctatccgaag cccgccgacg actacgtcca catgcggatg cagctgatga tcgaggcgct
     1141 ggcccaggcc atcgagaagg cgggcagcct ggacgcggtg gcggtcgcga gcgcgctgga
     1201 gaaggccagc gtcgatctgc acgggcaaca gggcttcgtg cgcgcgctgg accaccagtt
     1261 ccagcagcag ctggtggtgg gagtcatgga ccggcagggc gcaccgggcg tcaagttcga
     1321 cgtggaaggc tcgggctacg gctttcgcgt cgtcaagacc atcacgccgg cccaggcggg
     1381 tttgcccacc agctgcaaga tggtccgtcc aggctagatt cgggaaccag ccccaaggta
     1441 tttgccatgc gtcaagccat ccagaatctc gaagagtcca agatccgcga agtcgccaac
     1501 gccggcatgg ggcgcagcga cgtgctggcc ttctggttcg gcgaaggcga cgaggtcacg
     1561 cccgacttca tccgccaggc cgccatcgaa tcgctgcgca gcggcgagac cttctacgcc
     1621 cacaacctgg gcctgcccga gctgcgcgaa gccgtcgcgc gatacaccag cggcttgcac
     1681 ggccaagtcg ccgccaatcg cattgccgtc acctcgggcg gggtgaacgc cctgatgctg
     1741 gcggtgcagg cgctggtgga cgccggcgac gaagtggtgg cggtcacgcc ggtctggccc
     1801 aacctgacgg cccagccgct catcatgggc gcccggctca agcgcgtatc gctcaagccc
     1861 gacgccgggg cctggacgct cgacctgcag gcgctgctcg atgcagtcac ggcggacacc
     1921 cgcctgctgg tgatcaatgc gcccaacaac ccgaccggct ggacgctcac ccgggccgag
     1981 cagcaggcca ttctcgatca ttgccgcaag acgggcacgt ggatcctggc ggacgaggtc
     2041 tatgaacgcc tgttctttga accttcgccc aacggctgcg cccccagctt tctggacatc
     2101 gcatcaccgg acgaccggct ggtggtcgtc cacagcttct ccaagagctt cctgatgacg
     2161 ggctggcggc tgggatggct gatcatgccc gcagccatga cgccccacat gggcaagctg
     2221 gtggagttca acacctcctg tgccagcgtg ttcacccagc gtgccggcct ggtcgcgctc
     2281 gagcgcacgg atgaggtgac gccgcgggtc gtggcgcacc tcaagacctg ccgggatacg
     2341 ctggtgccgc tgctgcaagc tctgccgcgg gtcgagctcg aggcagccaa gggcggcatg
     2401 tacgccttct tcaagctcga cggttttggc gattcgctgg ccctggccaa gcggctggtg
     2461 gccgaagccg ggctgggcct ggcgcccggc aacgctttcg ccccggaagc ccagggctgg
     2521 ctgcgatggt gcttcgcgtc gaaggacgtg tcccggctgg agcggggggt ggaacggctc
     2581 agcggttggc tggccgcgag gaaatagccg gggacgccgt ggaaccggct ttgccgggcc
     2641 agtggcgtcg ccccccagtg gggggaggcc cggccgtacc cggccgcttc ggggggcgtg
     2701 ctaaaatctc atggctttgc atcgtgcaag gcgcacgttg gggcgcagtt ccagcctcca
     2761 acgcaagtta caacattcaa ggacaaacca atgaccgctt tgaacaaggc cgaagtcgta
     2821 aaggccaacg cgcgcagcgc gaacgacacg ggcagcccgg aagtacaggt cgcactgctc
     2881 acggctcgca tcaacgaact caccccgcac ttcaagacgc acgccaagga ccaccatggc
     2941 cgccgcgggc tgctgcgcat ggtgaatcgc cgcaagagcc tgctggcgta cctgaaggac
     3001 aaggacgccg agcgctacac cgcgctgatc ggcaaactgg gcctgcgcaa gtaaggcagg
     3061 atcaaacacc aaagcgcctg agttagtgga agtgcagaag cttccttgct ggctcaggcg
     3121 ctttgcactt cggagacggc aagacgggta caagtcattt cctaggggct agggattagg
     3181 gattagggat tagggattcg gccccctagt ccctagcccc taaccccaag cccctaagaa
     3241 atggcctcgt gttcgactcc gcctctccgg gcagcaagcg cagcctgatg cgctcttgaa
     3301 actggagaga cagtcatgag tattttcaac aaggtcacca agagcttcca gtggggctcg
     3361 cacaccgtca cgctcgaaac cggcgagatc gcccgccagt ccgggggcgc cgtcatcgtc
     3421 aacatcgacg acaccgtggt gctcgcgacc gtggtcgctt ccaagggcgc caagcccggc
     3481 caggacttct tcccgctgac ggtggattac atcgagaaga cctatgccgc cgggaagatc
     3541 cccggcagtt ttttcaagcg cgaagccaag ccgagcgaac atgaaaccct gaccagccgc
     3601 ctgatcgacc gcccgatccg cccgctgttc cccgaaggtt tcttcaatga agtgcacgtg
     3661 gtcatccaca cgttgtcgct gaaccccgaa gtcgacgccg acatcgccgc catgatcggc
     3721 accagcgcgg cccttgccat ctccggcatc ccgttcgccg gcccgatcgg cgccgcgcgc
     3781 gtgggctacg ttgacggcga gtacgtgctg aacccgggcc agaccgcccg caagctgtcg
     3841 gacctggacc tgatcgtcgc cggcactgaa agcgccgtgc tgatggtgga atccgaggcg
     3901 cagcagctgt ccgaggaagt gatgctgggt gccgtggtgt tcggccacga gcaaggcaag
     3961 atcgcgatca acgccatcca tgaactggtg cgcgacgccg gcaagccggt gtgggactgg
     4021 cagccgccgg cccgcgacga agccttcatc gccaaggtca acgcgctggc tgaagagaag
     4081 ctgcgcgccg cctaccagat ccgcagcaag caggcccgta cccaggccct gcgcgaagcg
     4141 ggcggcgccg tgcaagccga cctcaaggcc gagggtttcg aattcgaagc cgccaagctg
     4201 gacgccctca tgttcgacat cgaagcgaag atcgttcgca gccagatcct ggccggtgag
     4261 ccgcggatcg acggccgcga cacgcgcacc gtgcgcccga tcgaaatccg cacgggcgct
     4321 ctgccgcgca cccatggttc ggcgctgttc acgcgcggcg aaacccaggc gctggtcgtg
     4381 accacgctcg gcaccgagcg cgatgcgcag cgcatcgacg cgctgatggg cgagtacgag
     4441 gaccgcttca tgttccacta caacatgccg cccttcgcca ccggcgaagc gggacgcatg
     4501 ggcacgacca agcgccgcga gatcggccac ggccggctgg ccaagagggc gctgctcgcc
     4561 tgcctgccga ccaaggacga attcccctac accatgcgcg tggtgtccga gatcaccgaa
     4621 tccaacggtt cgtcttcgat ggcctcggtg tgcggcggct gcctgtcgct gatggacgcg
     4681 ggcgttccga tgaaggccca tgtggccggc atcgcgatgg gcctgatcaa ggaaggcaac
     4741 cgcttcgcgg tgctcacgga catcctgggc gacgaagacc acctgggcga catggatttc
     4801 aaagtggccg gcacgaccaa cggagtcacc gcgctgcaga tggacatcaa gatccagggc
     4861 atcacccgcg aaatcatgca ggtcgccctg gcccaggcca aggaagcgcg catgcacatc
     4921 ctgggcaaga tgcaggaaac catggccgaa gcgaagaccg aggtgagcaa ctacgcgccg
     4981 cgcctgttca cgatgaagat caaccccgag aagatccgcg acgtgatcgg caagggcggc
     5041 tccgtgatcc gcgcgctgac cgacgagttc ggctgccaga tcaacatcga ggaagacggc
     5101 accatcacca tcgcctccac cgatccgtcc agggcggagg acgccaggaa acgcatcgag
     5161 cagatcaccg ccgaagtcga gatcggcaaa gtctacgagg gcccgatcac caagctgctg
     5221 gacttcggcg cgctggtgaa cctgctgccc ggcaaggacg gcctgctgca catcagccag
     5281 atcgcgcatg agcgcgtgga aaaggtcacc gattacctga gcgaaggcca gatcgtgcgc
     5341 gtgaaggtgc tcgagaccga cgagaagggc cgcgtcaagc tttcgatgaa agccttgctc
     5401 gaccgtccgg ccatgccgcc gcgcgacgag caaccccggg gcgaccggca gccgcgaggc
     5461 gaccaacccc cgcgcgatca gcagttccgc gagcagcgcg accagccccc gcgcgatcag
     5521 cagttccgcg agcagcgcga ccaaccccca cgtgaccagc agttccgtga gcagcgcgac
     5581 cagcagcagc gcgagccgcg tgaagagcgc gacgtgccgc agcagacgcc ggtcgactcc
     5641 caggagtgat ggccgctctc gctttacctc gcatggcctg tccgcggcat cgatggcatg
     5701 tgttccattc gtcattgagc cgcgcagcgg aaaagtcatt cgtcattcgt cattcgtttt
     5761 cgcatgaaag ccgttgagat cagcgcctat ggcaagcccg aggtattgcg cctgggcgag
     5821 cggcccgcgc ccgtgcccgc ggcgggcgag ttgttgatcc gcgtctcggc cagcggcatc
     5881 aatcggccgg acgtgctcca gcgcaccggc aactacccgg tgccgccggg ggcttcggac
     5941 atcccgggcc tggaagtcgc gggcgagatc gtttcgggtg atgaaaacgc cctcgcacag
     6001 gccggcttca aggtcggcga ccgcgtttgc gcgctggtgg ccggcggcgg ctatgcgcaa
     6061 ctttgcgtcg caccggtcgg ccaatgcctg ccggtgcccc aaggcctgac cgacgtcgag
     6121 gcggcttcgc tgcccgagac ttttttcacg gtgtggagca acgtgttcga ccgcgtgaag
     6181 ctccagccgg gcgagacgct gttgatccag ggcggcacca gcggcatcgg cgtcacggcc
     6241 atccagatgg ccaaggcgct gggcgcgaag gtcatcgcca ccgccggcag cgacgagaag
     6301 tgccaggcct gcctcaagct cggcgcggat cacgcgatca attacaggac ccaggacttc
     6361 gccgcggagg cgaaaaagct cagcggcggc gccggcgtca acgtcatcct ggacatggtg
     6421 gcgggcggtt acgtcgcgcg cgaagtggag tgcctggccg aggacggccg gctcgtcatc
     6481 atcgccgtgc agggcggggt gaaaagcgag ttcaacgccg gcctggtgct gcgcaagcgc
     6541 ctggccatca cgggctccac gctgcggccg cgtccggtgg agttcaaggc ggccatcgcc
     6601 cgctcgctca aggaaaaggt ctggccgctg ctggaaaacg gcgccatcag gcccgtgatc
     6661 catagcgtct ttccggccgc gcaggcggcc agggcccatg aactcatgga atccaaccag
     6721 cacatcggca agatcgtgct gaactggacg gaggaaaaat gaaaaagaag ctgattgcgg
     6781 gcaactggaa gatgaacggc agcaccgcgg cgaacgaaga cctggtgcgc gcgctgatcg
     6841 ggggcatggg cgacgccgcc tgcaccgtgg cggtgtgcgt gccggcgcct tacctggccc
     6901 aggtgcagat gctgcgggcg ggctcgcggc tggaactggg tgcgcaggat gtgtccgagc
     6961 atgcggaagg cgcttacacg ggcgaaatct ccgccgccat gctcaaggag ttcggcgtgc
     7021 gctacgccat cgtcggccat tccgagcgcc gccagtacca cgccgaaacc gatgccatcg
     7081 ttgcggacaa gaccaaggcg gcgctggcgg ccggcatcac accgatcgtc tgtgtcggcg
     7141 agacgctggc gcagcgtgaa gccggccgga cggtggacat cgtcaagcgc cagatggccg
     7201 cggtgatcca caccaacggc cactgcatca gcgaaatcgt cgtcgcttac gaaccggtct
     7261 gggctatcgg caccggcagg accgcgacgc ccgagcaggc gcaggaagtc cacgccgtgc
     7321 tgcgcacgca gctcaaggcc gcgaccgagc atgtcgaccg cattcatatt ctttacggcg
     7381 gcagcatgaa cgccgccaat gcggcatcgc tgctggcgca gccggacatc gacgggggac
     7441 tggtcggcgg cgcctcgctc aaggccgccg attttctgca gatcatcgcc gccgcgcgct
     7501 agccgcgggc ggacacaaga atcaggagtg aacgccaatg aacaatgtat tgcttaccgt
     7561 gatcctggcc gtgcagatgc tcgcggcgct gtgcatggtc ggtctcatcc tcattcagca
     7621 cggcaagggc gccgacatgg gtgcggcctt cggcagcggc agctcgggca gcctgttcgg
     7681 cgccagcggc agcgccaact tcctgtctcg caccacggct gtgctcgcgg ccgtattttt
     7741 cgtctgtacc ctggcgctgg cttacttcgg caacctcagg ccggtggaat cgggaagcgt
     7801 tctggagcgc gccgccacga cccccgtgct gccggcgccg gcgcccgcgc cggccgcttc
     7861 cggcgccgcc gcccagatcc ccggcacggc tgcgccggcc cctgcctcta ccacaacagc
     7921 gcctgccgcg ccagcggctt ctggagccgg acagattccc gtcaactgat tccaagatcg
     7981 gtggggtggc tggaggagca actgtattta agggtaaact ccgaggctgt ccgggaagca
     8041 aatatcgaca gattcctcat gcctgtccgg gcagcaacaa cgccgtcgtg gtgaaattgg
     8101 tagacacgct atcttgaggg ggtagtggcg aaagctgtgc gagttcgagt ctcgccgacg
     8161 gcaccaaata atgaagctcg ccgggacgca tcggcgggct cgaagcacgg actcaggcca
     8221 agatgaatct cgaccagtat ctccctgttc ttctgttttt gctggtcggc gccggggtgg
     8281 gcgtcgctcc catggtcatg ggatggatgt tcagcagcgt gctcggcttc cagcggcccg
     8341 acgcggccaa gaattccccc tacgaatgcg gcttcgaggc tttcgacgac gcccgcatga
     8401 agttcgacgt gcgctactac ctcgtcgcca tcctgttcat cctgttcgac ctggagatcg
     8461 cgttcctgtt tccgtgggcg gtggccctga aggaaatcgg acctctcggt ttctgggcca
     8521 tgatggtctt cctgaccatc ctcgtcgtgg gtttcgtata cgaatggaaa aagggcgcac
     8581 tcgactggga gtgattcccc ggggcgcggc ttcaaggaag tattgctatg gctaacgaag
     8641 gcattctcga aagagtcccc gacaagagca ttctcgacag ggctccggac aaggggctgc
     8701 tcgacaaggg tctggtcacc acgacggtgg acaccgtcat caactgggcc aagaccggct
     8761 cgctctggcc gatgtctttc gggctcgcgt gctgcgcggt ggagatgatg cacgccggcg
     8821 cggcacgcta cgacatcgac cgtttcggca tgctgttccg tcccagtccg cgccagtcgg
     8881 acctgatgat cgtcgccggc acgctgtgca acaagatggc gcccgcgatg cgcaaggtgt
     8941 atgaccagat ggccgagccg cgctgggtca tctccatggg ctcgtgcgcc aacggcggcg
     9001 gctactacca ttacagctat tcggtcgtgc gcggctgcga ccgggtagtg cccgtggacg
     9061 tgtacgtccc cggctgcccg cccaccgccg aagcgctgct gtacggaatc atccagctgc
     9121 agcagaagat ccgccgcgtg cagacgatcg cccgggcctg aaagcgagac tttgatgacc
     9181 ctcttcgccg ttgatcccca agccctcaag gaaacgctgg ccaccacgct cggtgcgctg
     9241 gccaggagaa tcgacctcaa gctgggcgag gtcaccgtgg tggttgcgcc cgctggttac
     9301 ctggaggccg cccggctgct gcgcgatacg cccggttgcc agttcgagca gcttgtggac
     9361 ctgtgcggcg tcgactactc cgaataccgc ggcggcggct gggaaggccc gcgctactgc
     9421 gtggtgtcgc acctgctctc ggtgaccctg aaccagcgcg tgcgcctgaa ggtgttcgcg
     9481 cccgacgacg aactgccggt gctggactcc ctcaccggcc tgtggagctc ggccaactgg
     9541 ttcgagcgcg aggcgttcga cctgttcggc gtcatcttcg agggtcaccc cgacctgcgc
     9601 cgcatcctga ccgactacgg cttcatcgga cacccgttcc gaaaggactt cccgatggag
     9661 ggccacgtcg agatgcgtta cgacgcggag cgcggccgcg tgatctacca gccggtgacg
     9721 atcgagccgc gcgagatcac cccgcggatc atccgcgaag agaactacgg agggctgcac
     9781 taggacgctt ctgtcattgc gggcttgacc cgcaatccac gctggcgcaa cagcatggat
     9841 gccggatcaa gtccggcatg acaagcccgg cagaaccaaa tggcagaaat caagaactac
     9901 accctcaact tcggtcccca gcatccggcg gcgcacggtg tgctgcgcct ggtgctcgag
     9961 ctcgacggcg aagtcatcca gcgcgccgac ccgcacatcg ggatgctgca ccgggccacc
    10021 gagaagctgg ccgagaccaa gacctacctc cagacgctgc cctacatgga ccgtctggac
    10081 tacatgtcga tgatgtccaa cgagcagggc tattgcctcg ccgtcgagaa gctgctgggc
    10141 gtcgatgttc cggtccgcgc ccagtacatc cgcgtcatgt tcgccgagat cacgcggctg
    10201 ttgaatcacc tgctgtggct gggcgcatcg gggctcgact gcggcgccat gaacgtgctg
    10261 ctttatgcct tccgcgaacg cgaagacctg ttcgacgtct acgaggcggt ttcaggcgcg
    10321 cgcatgcacg ccgcgtactt ccgaccgggc ggggtctatc gcgacctgcc ggacaccatg
    10381 ccgcagtaca agcccagcaa gatcaagagc gagcgcgcga tcaagaggct gaacgagaac
    10441 cgccagggct ccgtgctgga cttcatcgac gatttcacga agcgcttccc gcattacgtg
    10501 gacgagtacg aaaccctgct caccgaaaac cggatctgga agcagcgcac cgtgggcatc
    10561 ggtgtcgtga gtcccgaccg cgccttgaac ctgggcttca cgggcccgat gctgcgcggt
    10621 tccggcgtcg catgggattt gcgcaagaag cagccctacg aggtctacga ccggctcgat
    10681 ttcgacatcc ccatcggcag gaacggcgac tgctacgacc gctacctggt gcgggtgcag
    10741 gagctgcgcc agtccaaccg catcatcaag cagtgcgtgg actggctgcg ggcgaacccc
    10801 ggacccgtca tcaccgacaa ccacaaggtg gcgccgcccg accgcgagtc gatgaaggcc
    10861 aacatggaag agctgattca ccacttcaag ctcttttcgg aaggaatgcg ggtgcccgaa
    10921 ggcgaggcct atgccgccat cgaacatccc aagggcgaat tcgccgtcta catcgtgagc
    10981 gacggcgcca acaagcccta tcgcttgaag atccgcccgc cgggattcgt tcacctgtcc
    11041 gcgatggacg aaatggcgcg cggccacatg atcgccgatg ccgtggcggt aattggcacc
    11101 ctggacatcg tgttcggaga aatagaccg
//
LOCUS       DASWIY010000417         6226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_286261, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000417 DASWIY010000000
VERSION     DASWIY010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286261"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1398
                     /locus_tag="VGA59_14745"
     CDS             82..1398
                     /locus_tag="VGA59_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX9720975.1"
                     /translation="MKRPNIVFIVADDLGYADLGCYGGRPAEFGAVSPVLDGLAADGL
                     KFTQGYANSPVCSPTRFALMTARYQYRLRGALEEPIRTSSRGNMRLGLPPEQPTLPSL
                     LKASGYRTALIGKWHLGYPPSFGPLRSGYEEFFGPMSGGVDYFTHCSFTGDHDLWFGE
                     DEKREEGYLTDLLSHRAVDYVTRMAAQRAPFFLSLHYTAPHWPWETRDDQALAPEVKG
                     NLFHLHGGNVHTYRRMIHHMDEGIGWVMDTLRATGLADDTLVVFTSDNGGERFSDNWP
                     LVGGKMDLTEGGIRVPWIAHWPAVIAAGRVSQQHCMTMDWSATLLEAGGARADPQYPL
                     DGVSLMPTLRQPADNVRRPLYWRMNHRGQRALRDGDWKYLRVDGNDYLFNIPADARER
                     ANLAPREPQRLAAMRETWEQWNATMPPIPEDATVSLGYSAKDMPQR"
     gene            1459..3156
                     /locus_tag="VGA59_14750"
     CDS             1459..3156
                     /locus_tag="VGA59_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX9720976.1"
                     /translation="MPNLSRWFPFLNWPRPDAALLRGEFGAGLTVAVVMIPQSVAYAG
                     LAGMPLITGLYATFLPALAAVLFSGSTRLSVGPSALTSVLVGASLIGLAEPGSGQWIA
                     LAVWLALLSGAIQLGLGAARAAWVLNLVSSPVLAGFSQAAALLIIASQIPTLIGLQGP
                     LSSLWQAPQFDLTALGYGGVSLLLFILSKRYAPRLPIVLLVVAAAGALGKWSGYSAHG
                     AVIGTLPAGLPAPYWPGLPGWHTLGALVVPAMVIALVSSLEMAASAKIESQRDGKRWD
                     AGQDLIGQGMGKLAAAFSGSFPTSTSFSRSAITLYAGARTGWATVLATGVVLLVLLFL
                     TPALYHVPRAVLAAVVVTAVAGLFRPHTLMRLWKIDRVEALTGLVTFAVTAITAPRIY
                     WGVLAGVMMGLAHFLYLRLHPRIIEVGLHPDGSLRDRHLWQLPPLAPQLYAMRMDAEL
                     DFASASALERAVVEHLAAHPQVRHLCLFAHPINRVDATGVEVFGQLRRLLDERGIRLH
                     ISGLKLPVERVLRKSGVLYDAPLLNVYRTDAEALLAFAQLSADAPLPDPANPDHVTP"
     gene            complement(3146..3964)
                     /locus_tag="VGA59_14755"
     CDS             complement(3146..3964)
                     /locus_tag="VGA59_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEX9720977.1"
                     /translation="MAEPLLLGCDFSSSPTRRKPIVLALGSQAGGRVVLAGLEKIDTL
                     AGFAGWLAQPREWVGGFDFPFGLPRELVEHLGWPLQWRDCIAHYASLSRLDIRTMFAA
                     FCDARPAGSKFAHRAFDKLAGSSPSMKWVNPPVAYMLHAAVPALLAAGIHIPGLHAGD
                     RRRVALEAYPGLLARELIGRRSYKSDEKARQTPDRLIARKDLLTALEIGRTRLDLRLK
                     LTHAQRDALAQDASGDSLDAVLCLVQAAWAQRHGAPRYGLPQRLDSLEGWIITA"
     gene            complement(4053..4475)
                     /locus_tag="VGA59_14760"
     CDS             complement(4053..4475)
                     /locus_tag="VGA59_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HEX9720978.1"
                     /translation="MLVRLLYASRAVDISPEAIESILAQSRQHNPTCGITGILCYGGG
                     IFLQAIEGGRTQVSELFGHIQKDPRHRDVALLHYEEISERRFGGWSMGQVNLTRLNHA
                     TLLKYSEKPELDPYAVSGKVSLALLEDLIATAAICGRA"
     gene            complement(4519..5250)
                     /gene="folE"
                     /locus_tag="VGA59_14765"
     CDS             complement(4519..5250)
                     /gene="folE"
                     /locus_tag="VGA59_14765"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX9720979.1"
                     /translation="MNLAEPRAEGSVVADAGTPVSVKIRERILAARKRFHSNDNIADF
                     IQPGELEQLQLEVEGKMQGVLDSLVIDTSSDHNTSETARRVAKMYVSEVFKGRYVKPP
                     TITEFPNAERLNELMIVGPIAVRSACSHHFCPVIGKLWIGVMPNEHTNVIGLSKYARL
                     AEWVMGRPQIQEEAVVQLADLIMEKTQPDGMALVMEASHYCMAWRGVKDMDAKMLTSV
                     MRGVFLKDSALRREFLALIPGRTQI"
     gene            complement(5355..>6226)
                     /locus_tag="VGA59_14770"
     CDS             complement(5355..>6226)
                     /locus_tag="VGA59_14770"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427321.1"
                     /note="catalyzes the ATP-dependent formation of a
                     phosphodiester at the site of a single strand break in
                     duplex DNA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX9720980.1"
                     /translation="EGGGEGGPAPFALLQQRIGRKTLTKKVLADAPVTFVAYDLLEQD
                     RQDIRSLSQRERRARLEALLQGTGFKVSPVETDSSWLEFARRREQSRERGVEGFMLKH
                     LASAYGTGRQKRGQSPNSPAAKLESDPIYVWWKWKIEPMTIDCVLIYAQAGHGRRASV
                     YTDYTFAVWNRAPHSRQEAEAAIEAIARKEPPQPGALQLVAFAKAYSGLTDEEFRQVD
                     AIIRKSTLEKFGPVRSCKPSLVFELGFEGINRSPRHKSGIAVRFPRMLRIRLDKPLHE
                     ANTLQDLESLLKT"
BASE COUNT          957 a         2082 c         2074 g         1113 t
ORIGIN      
        1 tgtcgctgca aactgccggc agcgtctcgg tcgcgttttg ttcccggaga attccatgtt
       61 cacgcatccc agcccaatcg aatgaaacgg cccaacatcg ttttcatcgt tgccgatgac
      121 ctcggctacg ccgaccttgg ctgctacggc ggccggccgg cggagttcgg cgccgtatcg
      181 ccggtactcg acggtttggc ggccgatggg ctgaagttca cgcagggata tgccaactcg
      241 cccgtgtgct cgccgacgcg attcgcgttg atgacggctc gttaccagta ccgcctgcgc
      301 ggcgcgctgg aagagccgat caggacttcc agccgcggca acatgcggct gggcctgccc
      361 cccgagcaac ccaccctgcc gtccttgctc aaagcgagcg gctaccgcac cgcgctcata
      421 gggaagtggc acctgggcta tccgccgtcg ttcggcccgt tgcgctcggg ctacgaggaa
      481 tttttcgggc cgatgtcggg cggtgtggac tacttcaccc actgctcgtt caccggggat
      541 catgacctgt ggttcggcga ggacgaaaag cgggaagaag gctatcttac ggacctgctt
      601 tcgcaccgcg cggtcgatta cgtgacgcgc atggcggcac aacgcgcgcc gttcttcctc
      661 agcctgcact acaccgcgcc gcattggccc tgggaaacgc gcgacgacca ggcgctggcg
      721 ccggaagtga aaggcaacct gttccacctg cacggcggga acgtccacac gtaccgccgc
      781 atgatccatc acatggacga aggcatcggc tgggtgatgg atacgttgcg cgcgaccggc
      841 cttgcggacg acacgctcgt ggtgttcacc agcgacaacg gcggcgagcg tttctccgac
      901 aactggccgc tggtcggcgg caagatggac ctgaccgagg gcggtatccg cgtgccgtgg
      961 atcgcgcact ggccggcggt gatcgcggcc ggccgggtga gccagcaaca ttgcatgacg
     1021 atggactggt ccgccacgct gctggaagcc ggcggcgccc gagccgatcc ccagtatccg
     1081 ctcgacggcg tgtcgctgat gccgacactg cggcaacccg ccgacaatgt tcgccgcccg
     1141 ctgtactggc gcatgaatca tcgcggccag cgcgcgctgc gcgacggcga ctggaaatac
     1201 ctgcgtgtgg acggcaacga ctatctgttc aacattccgg ccgacgcacg agagcgcgcc
     1261 aacctggcgc cgcgcgagcc ccaaaggctg gccgcgatgc gcgagacctg ggagcagtgg
     1321 aacgcaacca tgccgccgat ccccgaggac gccaccgtga gcctgggcta ttcggccaag
     1381 gacatgccac agcgctaggc gatccggcta cgtgtgttcc gcatgaggcg cggcgcggcg
     1441 cacaggcata ctttggccat gccgaatctc tcccgctggt ttccgttcct caactggccg
     1501 cgccccgatg cggccctgct gcgcggggaa ttcggcgccg gcctcacggt ggccgtcgtg
     1561 atgattccgc agtcggtcgc ctatgcgggc ctggccggca tgcccttgat caccgggctg
     1621 tatgcgactt tcctgcccgc gttggcggcc gtgctgttca gtggttccac ccggctgtcg
     1681 gtcggaccgt cggcgctaac cagcgtgctg gtcggcgcct cgctgatcgg gctggccgag
     1741 ccgggtagcg gccagtggat cgcgctggcc gtgtggctgg cgttgttgtc gggggcgatc
     1801 cagcttggcc tgggcgccgc acgcgcggcc tgggtcctca atctggtgag ctcaccggtc
     1861 ctggccggct tcagccaagc ggcggcgctg ctgatcatcg cctcgcagat tcccacgctg
     1921 atcgggttgc aggggccgct gtccagcctg tggcaggcac cccagttcga cttgacggcg
     1981 ttgggctacg gcggggtcag cctgctgctt ttcatcctga gcaagcgcta cgcgccgcgc
     2041 cttccgattg tcctgctcgt cgtcgccgcc gccggcgccc tcggcaaatg gagcggctac
     2101 tccgcgcacg gcgcagtgat cggcaccttg ccggcggggc tgccggcgcc ctattggccg
     2161 ggtctgccgg gctggcatac gctgggcgcg ctggtggtgc cggcgatggt catcgcactg
     2221 gtgagctcgc tcgaaatggc ggccagcgcc aagatcgaga gccagcgcga cggaaagcgc
     2281 tgggacgccg gccaggatct catcggccag ggaatgggca agctggcggc tgcgttctcc
     2341 ggcagcttcc ccaccagcac ctcgttctcg cgctcggcga tcaccttgta tgcgggagcg
     2401 cgcaccggct gggccacggt gctcgcaacc ggtgtggttc tgctggtgct gctgttcctc
     2461 accccggctc tctaccatgt gccgcgcgcg gtgctcgcgg ccgtggtcgt gacggcggtg
     2521 gcaggcctgt tcaggccgca cacgctgatg cgcctgtgga agatcgatcg ggtcgaagcg
     2581 ctcaccgggc tggtgacctt tgcggtcacc gcgatcacgg cgccaagaat ctattggggc
     2641 gtgctggccg gcgtgatgat gggcctggcg catttcctgt acctgcgcct gcatccgcgc
     2701 atcatcgaag tcgggctgca cccggacggc agcctgcgcg accggcacct gtggcagctg
     2761 ccgccgctcg cgccgcagct gtatgcgatg cgcatggatg ccgaactcga cttcgcctcg
     2821 gccagtgcac tggagcgggc cgtggtcgaa cacttggccg cgcacccaca ggtgcggcat
     2881 ctgtgccttt tcgcgcaccc catcaaccgc gtggacgcga ccggggtcga agtgttcggc
     2941 cagttgcgca gactcctcga cgagcgcggc atccgcctgc atatcagcgg gctgaagctg
     3001 ccggtcgaac gcgtgctgcg caagtccggt gtgctctacg acgcgccgct gctgaacgtg
     3061 taccgcaccg atgccgaggc gttgctggcc tttgcgcagc tcagcgctga cgccccactc
     3121 ccggaccccg cgaacccgga ccacgtcacg ccgtgatgat ccaaccctcg agcgaatcga
     3181 gccgttgcgg caatccatag cgcggcgcgc cgtgccgctg cgcccatgcg gcctgcacca
     3241 ggcacagcac ggcgtccagg ctgtcgccgc tggcgtcctg ggccagcgcg tcgcgctgcg
     3301 catgggtgag tttcaggcgc aagtccagcc tcgtgcgccc gatctcgagt gccgtgagca
     3361 ggtccttgcg tgcgatcagg cggtcgggcg tctgcctggc cttttcgtcg ctcttgtaac
     3421 tgcgcctgcc gataagctcg cgcgcgagca ggccgggata ggcctccagc gccacgcggc
     3481 gccggtcgcc cgcgtgcagc cccggaatgt ggatgccggc ggccagcagc gcgggcacgg
     3541 ccgcgtgcag catgtaggcc acgggaggat tgacccattt catcgagggg ctggagcccg
     3601 ccagcttgtc gaaggcacgg tgggcgaact tggacccggc gggccgggca tcgcaaaacg
     3661 ccgcgaacat tgtgcggatg tccaggcgac tcaggctcgc gtagtgggcg atgcaatcgc
     3721 gccactgcag cggccagccc aggtgctcga ccagttcgcg cggcaggccg aagggaaagt
     3781 cgaagccgcc cacccactcg cgcggctgcg ccagccaccc ggcgaagccc gccagcgtgt
     3841 cgatcttctc gagcccggcg agcacgaccc ggccgccggc ctgcgacccc agcgccagca
     3901 cgatgggctt gcggcgggtc ggactgctgg agaaatcgca acccagaagc agcggttcag
     3961 ccatacgaaa gctcaagtgg gggtcggcac cttcacgctg cccgcaatcg atgcgcggcg
     4021 gtcaggaacg cgccgcagcc tgtgaacagg ccctaggccc gcccgcagat ggccgcggtg
     4081 gctatcaggt cttccagcag cgcgagcgaa accttgcccg aaaccgcgta ggggtcgagc
     4141 tcgggctttt cggagtactt cagcagcgtc gcgtgattca gcctggtcag gttcacctgt
     4201 cccatgctcc agccgccgaa gcgccgctcg gaaatctcct cgtagtgcag cagcgcgacg
     4261 tcgcggtggc gcgggtcttt ctggatatgg ccgaacagct cgctgacctg cgtgcggccg
     4321 ccttcgatgg cctgcaggaa gatgccgccg ccgtagcaca ggatgccggt aatgccgcag
     4381 gtggggttgt gctggcgcga ttgcgccagg atggattcga tcgcctccgg gctgatgtcc
     4441 accgcccggc tggcatagag aagtcgcacc agcatggtca tgcctcccga cgcgcggcaa
     4501 ggccgcaaaa gtgcgggctc atatttgtgt ccttccggga atgagggcca ggaattcacg
     4561 gcgcagcgcc gaatccttga ggaacacgcc gcgcatgacc gaagtgagca tcttggcgtc
     4621 catgtccttg acgccgcgcc aggccatgca gtagtggctc gcctccatca ccagtgccat
     4681 gccgtcgggc tgggtttttt ccatgatgag gtcggccagc tggaccacgg cctcctcctg
     4741 gatctggggc ctgcccatca cccattcggc caggcgggcg tatttggaaa gcccgatgac
     4801 gttggtgtgc tcgttgggca tgacgccgat ccagagcttg ccgatcaccg ggcagaaatg
     4861 gtggctgcag gcgctgcgca cggcgatggg gccgacgatc atcagctcgt tgaggcgctc
     4921 ggcgttcgga aattcggtga tggttggcgg cttgacgtag cgccccttga acacctcgct
     4981 gacgtacatc ttcgccacgc gccgcgccgt ttcgctggtg ttgtggtcgc tgctggtgtc
     5041 gatcaccagg ctgtcgagca caccttgcat cttcccctcg acctccagct gcagttgctc
     5101 gagctcgccc ggctggatga aatccgcgat gttgtcgttg gaatggaagc gcttgcgcgc
     5161 ggccaggatg cgctcgcgga tcttcaccga gaccggcgtg ccggcatcag cgacaacgct
     5221 gccttcggcg cgaggttcgg ctaggttcat ggacggttcg gctttcatga gcatgcgaaa
     5281 tcgtaacact gcctgctttc ggcgggtttg acaagggtct tggcgcacgg cccttgtcca
     5341 gccggcgaca ctcgctatgt tttcaggagc gattcgagat cctgcagggt gttggcctca
     5401 tgcagcggct tgtccaggcg gatgcgcagc atcctcggaa agcgcacggc gatgccgctc
     5461 ttgtggcgcg ggctgcggtt gatgccctcg aagcccagct cgaacaccag cgaaggcttg
     5521 cagctccgca cggggccgaa cttctcgagc gtcgacttgc ggatgatcgc atcgacctgc
     5581 cggaactcct cgtcggtgag accggagtac gccttcgcga acgccaccag ctgcagcgca
     5641 cccggctgcg gcggctcctt gcgtgcgatc gcctcgatcg ccgcttcggc ctcctgcctg
     5701 ctgtgcggtg cgcggttcca cacggcgaag gtgtagtcgg tgtagaccga ggcgcggcgg
     5761 ccatggccgg cctgcgcgta gatcagcacg caatcgatcg tcatcggctc gatcttccac
     5821 ttccaccaga cataaatggg gtcggattcc aatttcgccg caggcgaatt cgggctctga
     5881 cccctttttt gtctgccggt gccgtaggcg ctggccagat gcttgagcat gaagccttcc
     5941 accccgcgct cgcgcgactg ctcgcgccgg cgggcgaact ccagccagga agaatcggtt
     6001 tcgacgggcg acactttgaa tccagtaccc tgcaagagcg cttcgagccg cgcgcgccgc
     6061 tcacgctggc tcagggagcg aatatcctgg cggtcctgct ccagcaagtc gtaggcgacg
     6121 aaagtcaccg gcgcatccgc cagcaccttt tttgtgagcg tcttgcggcc gatgcgctgc
     6181 tgcagcaacg cgaacggtgc ggggccgccc tcacccccac cctctc
//
LOCUS       DASWIY010000418        20431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3251816, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000418 DASWIY010000000
VERSION     DASWIY010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 20431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20431
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3251816"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1844)
                     /locus_tag="VGA59_14775"
     CDS             complement(<1..1844)
                     /locus_tag="VGA59_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX9720981.1"
                     /translation="MFEFVRKHLKIMQLVLFLLIVPSFVLVGISGYDRFQDKGEAVAT
                     VDGEEIRQGDWDAAHKQEVERLRQSMPTLDPKLLDSPAARYATLERMVRDRVLAAAAA
                     KSKLVTGDQRLARELQQNELIASLRGPDGKLDMARYRQLVSSQGMTPEMFEASVRADL
                     SARQVLGGVGGTSLATPGQANLSLNALFEKREVQVARFAAPDYAGRVKPTDAELEAYY
                     KANAALFQAPEQAGIEYMVLDLDTIKKGMTVNEQDLKTYYEQNAGRLSGQEERRASHI
                     LVAVPKGAPAPEREKAKARAEELLAAVRKSPGTFAEVARKDSQDPGSAANGGDLDFFG
                     RGAMTKPFEDATFALKKGELSGLVETEFGYHIIMVTDIKAPKQRSFEDMRPELEADLK
                     KQQAQRKYAEAADTFSNGVYEQADSLKPVADRLKLEIRTASNVTRTPSPGASGALANP
                     KFLTALFSPDSTQKKRNTEAVEIAPNTLVAGRVTQYTPARTLPLAEVKERVRERLVAA
                     RAAELAKKEGMEKLAAWKANPAGAGLPAAIAVSRQETQKQPQAVVEAALRADPAALPV
                     LAGVDLGDQGYAVVKVNKVLPREAPTEDVARQERQQYAQWWASAENLA"
     gene            complement(1969..2044)
                     /locus_tag="VGA59_14780"
     tRNA            complement(1969..2044)
                     /locus_tag="VGA59_14780"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2009..2011),aa:Val,seq:tac)
     gene            complement(2065..2337)
                     /locus_tag="VGA59_14785"
     CDS             complement(2065..2337)
                     /locus_tag="VGA59_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9720982.1"
                     /translation="MNKTELIEHIAKHADISKAAATRALESMIGAVKTTLKKGGAVSL
                     VGFGTFAVGKRAARSGRNPRTGAAIKIKAAKVPKFRPGKALKDALN"
     gene            complement(2631..3716)
                     /locus_tag="VGA59_14790"
     CDS             complement(2631..3716)
                     /locus_tag="VGA59_14790"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX9720983.1"
                     /translation="MARKRIAVIAGDGIGKEVMPEGIRVMEAAAGRFGIDLAFDHFDF
                     SSWDYCEKHGKMLPDDWKDRIGGHDAIYFGAVGWPEKIPDHVSLWGSLLLFRREFDQY
                     VNLRPARLMPGIIAPVVRRDGSARQPGEIDMYIVRENTEGEYSSIGGRMYPGTEREIV
                     MQETVMSRVGVDRVLKFAFELARSRPKKHLTSATKSNGIAITMPFWDERVAEMAKAYP
                     GVNVDKFHIDILTAHFVQRPDFFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPD
                     RKFPSLFEPVHGSAPDIAGRNIANPIGQIWCGAMMLDFLGYRQAHDAVLSAIERVLQP
                     GSGAPRTADLGGSATTSDLGRAIASAL"
     gene            complement(3743..4627)
                     /locus_tag="VGA59_14795"
     CDS             complement(3743..4627)
                     /locus_tag="VGA59_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9720984.1"
                     /translation="MLASFESFEVVRDGVRLHGRRGGQGEPLLLLHGHPQTHAMWHRV
                     APGLARAYSLVMMDLRGYGDSGRPDAGEGSCAYSKREMALDALAVMQEQGFARFKVLA
                     HDRGARVAHRLAADHPKAVDRLMLLDIAPTLAMYERTSLAFAQAYWHWFFLVQPPPLP
                     EALIESDPVRYVRSVMGSRHAGLAPFAPEALAEYERCAGIAGTAQGICADYRASAGID
                     MAHDRADVAADRRLEQPLRVLWGEHGAVGRCFDVLGLWRERARQVTGRSLPCGHYIAE
                     EAPALLLEEALQFFSASH"
     gene            4704..5666
                     /locus_tag="VGA59_14800"
     CDS             4704..5666
                     /locus_tag="VGA59_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9720985.1"
                     /translation="MNGLLDRGDLELLLAVRAHGSLAATAAAAGVVPSVVTKKLAALE
                     VRLGFRLFQRTTRRIVATAEGEALCERAAQLVQAFSQLETDLRERHSEPSGLIRLTAT
                     FGFGRLWLGPALADFQARHPLLRVQLQLTEQLPNLAVEGYDGAVWLWSVQGRRATEWV
                     GRRLARNQRVLVAAPQYLRQRGAPQTPADLAQHDCLIVRESDQRFDIWALRKERDKVE
                     VRARVQGPLSSNSGELVRDWCLAGRGIMLRSLWDIAPQLASGQLVRVLPGYAMFDADI
                     HWLAPHRAQTPKRVRLLIDFLAERFRGEPWKLKPAAAKADAPPG"
     gene            complement(5626..6519)
                     /locus_tag="VGA59_14805"
     CDS             complement(5626..6519)
                     /locus_tag="VGA59_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9720986.1"
                     /translation="MSSLSRQDALVRAMPAIFVVIWSTGFIVARYGMPHAPPMKFLAL
                     RYFLSVACFVAWATAAGVALLPRERGQVKHLAVTGILMHAGYLGGVWAAVKLGMGAGL
                     AALLVGLQPVLTAVWVSSRGGVVSARQWAGLALGFGGLALVVWQKLGVGEISGANFLL
                     AVGALLSITAGTIYQKRFLAPCDVRTASLVQLCAAFAVTLPLAVLETESMRWNGELAG
                     AMAWSVLALTLGGSSLLYLLIHRGAATAVSSLMYLVPPCTAMLAWLLFGEAITLLTIT
                     GIALAALGVSLVVRQPSPQRA"
     gene            complement(6524..7090)
                     /gene="pgsA"
                     /locus_tag="VGA59_14810"
     CDS             complement(6524..7090)
                     /gene="pgsA"
                     /locus_tag="VGA59_14810"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX9720987.1"
                     /translation="MFLTIPTILTWTRIVAIPLIIGVFYLGLDPALQNLVATAMFVVF
                     ALTDWLDGYLARRLNQMSSFGAFLDPVADKFLVCASLLVLVHLKRADVFVALIIIGRE
                     IAISALREWMAQIGASKSVAVHMLGKLKTVVQMIAIPFLLFDGLLFGVIDTGLWGTWL
                     IWIAAILTVWSMVYYLQKALPEIRKRVK"
     gene            complement(7174..9165)
                     /gene="uvrC"
                     /locus_tag="VGA59_14815"
     CDS             complement(7174..9165)
                     /gene="uvrC"
                     /locus_tag="VGA59_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX9720988.1"
                     /translation="MSEVHSDQLLAQVAALPALPGVYRYFDAQGTVLYVGKARNLKKR
                     VCSYFQKNHGGTRIGHMIGKIARLETTVVRSEAEALLLENNLIKTLNPRYNILFRDDK
                     SYPYLKISGPEKGGLSETAAVSAATFPRVAYYRGAVDRKHRYFGPYPSAWAVKESIQL
                     IQKVFRLRTCEDTVFANRTRPCLLYQIKRCSGPCVNLVSPQDYRHDVHNAEAFLRGET
                     QAVLAALEARMMQHAQKLEFEQAAELRNQIGALSKVLHQQSVETISDKDVDILAVKVQ
                     GGKACVNLAMVRGGRHLGDRPYFPSHIEDAAAVQDFDSRVDEGLEVAPPMPVEVQILE
                     AFIAQHYIGVPVPPALITSEPVGRELIDALSEQSGLKISAVHNPREQRRLWLEMAQKN
                     AAIQLARLLAEEGSQQARTRALVEALDLAPDNLDSLRIECFDISHTAGEATQASCVVF
                     HHHKMQNAEYRRYNIGGITPGDDYAAMRQVLLRRYSKLAEAGRDSPDATPPDGAGGAA
                     GAGGAGRLPDLVLVDGGKGQVSMAREVFAELGLDLAVIIGVEKGEGRKVGLEELVFAD
                     GRDKVYLGKDSAALMLVAQIRDEAHRFAITGMRARRARVRVGGSQIEDIPGIGAKKRA
                     RLLQRFGGVRGVAAAGVDDIASVEGISRELAEEIYRALH"
     gene            9211..10299
                     /gene="earP"
                     /locus_tag="VGA59_14820"
     CDS             9211..10299
                     /gene="earP"
                     /locus_tag="VGA59_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEX9720989.1"
                     /translation="MLWDIFCKVIDNHGDIGVCWRLSTQLAGRGEQVRLWVDDGLALG
                     WMAPHGCAGVQVLPWAAGSQPREPGAVVIEAFGCELAPAFQATLAARTRKEGHQPVWL
                     NLEYLTAESFAERNHGLPSPVLAGPAAGLTKYFFYPGFTPGTGGLLREPGLLERQERF
                     DREAWLDRHGIHVGKGCVLVSLFGYEPAALDQLLAHWARGPGRTHLLVTAGRAAAAVH
                     AAVARKAQSEPQWNRSAALTTSWLPLLTQLDFDHLLWACDLNFVRGEDSLVRALWAGK
                     PLVWQIYPQHDDAHHAKLMAFLDWLEAPALLREFHLAWNGVGGALPAASPDTWGECVQ
                     RGRLRLAAQDDLATQLMRFVASPGRAPA"
     gene            10296..10955
                     /locus_tag="VGA59_14825"
     CDS             10296..10955
                     /locus_tag="VGA59_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9720990.1"
                     /translation="MRLLLVEDDAMIGRAVRQGLGDAGFTVDWATDGRAAELALANGV
                     YDLAVLDLGLPGKDGMTVLRELRARRDPLPVLIVTARDAVPDRIEGLNAGADDYVLKP
                     FDLDELVARVRALLRRQAGGAVPVLQAGELTLDTVRREVFLEGREIRLSAREFALLEA
                     LMRRPGAVLSRERLEDAVYGWGEEVGSNAVEVHLHNLRRKLGAETILNVRGVGYKVID
                     K"
     gene            10952..12289
                     /locus_tag="VGA59_14830"
     CDS             10952..12289
                     /locus_tag="VGA59_14830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9720991.1"
                     /translation="MTASIHRRVLIGAFSALALGAGLLVGGSWWLLAQEMNEVFEDNL
                     KQVALAVASHHDAGAMTGPPRLAQQLPRVYEEYGKFEFVTQAWSREGKLLHRSDEGAA
                     LPFLSRSGLSTVAVGGEAWHLYTIVLEDGVVQAAQRASERQTLARETASALILPALAM
                     LAAIAGLLALALRRGLAPLSSAAGEVTTRSVEALHPIALASQPAELHLLVGAINDLMA
                     RLGSALALQRNFLADAAHELRTPIAALRLQLQLLDRARGEDQRAAAQDELRAGIARAQ
                     HLVEQLLQLSRLGPETPALKQEMVDLAELARAAVARFSARAEQQRIDLGAIAANEAMI
                     QGDAQHLTILVNNLVDNALRHTPAGGKVDVCAEPRQGQPCLIVTDSGPGIPAAERERV
                     FDRFYRSADAPPSTGSGLGLAIVRAVAQRHGAEVTLGEAPGGGLSVSVLFPSP"
     gene            complement(12264..13952)
                     /locus_tag="VGA59_14835"
     CDS             complement(12264..13952)
                     /locus_tag="VGA59_14835"
                     /inference="COORDINATES: protein motif:HMM:NF028537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HEX9720992.1"
                     /translation="MRPVEASPTARGRPSVHPEMLVLGVAVFLMAIGNGPFWRAALAG
                     RSWGELATWGFAGSLLAAFTAFYLAAAAAVAGRHTVKPLLTALLLVSAGASYYMDRYS
                     IYVDRAMLRNVLATHYKEATELLGSGLLLHLLAWGVLPSLLVWWPQLKRRTFGCAVMV
                     RLAWVSGALAIGIASLLPVFADFASLMRNHKEVRYLITPGNLVAAAAANAWGRARMPN
                     RPRLPVGADARMGAAWDGRTRPTLFVLVVGETARAKNFSLNGYGRETNPQLARHGVIN
                     FPHVRACGTSTEVSLPCMFSQFGRRHYDEDEILTHESVLHVLARAGFKVLWRDNQSGC
                     KGVCDGLAQEQLDRASTEGLCAQGQCLDEVLLQGMEGIARDSRGNLFVVMHQLGSHGP
                     AYFKRYPAALKRFQPACESEDLRRCSPAEIVNAYDDSLLYTDFFLGKVIDFLERAQET
                     HDTAMLYVSDHGESLGEGGLYLHGMPYAIAPDVQKDVPFVVWLSPAFRAGFGIDQECM
                     RGRAVEPASHDNLFHSLLGVLDVETAAYDRRLDLFAPCRETSPPSGPRVRETAH"
     gene            complement(13939..14598)
                     /locus_tag="VGA59_14840"
     CDS             complement(13939..14598)
                     /locus_tag="VGA59_14840"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9720993.1"
                     /translation="MASLAVLLAWDAAGLDLPLARLMAGGGGFSLREHWALAGVLHQG
                     GRQLGWLMALWLLVGVWWPTGWLRQLARPARLQWLASALLSLVLISALKYSSQTSCPW
                     DLSEFGGSASYLSHWAWGKSDAGPGRCFPAGHASAAFAFIGGYFALRQVSRRRAMLGL
                     AAVLAAGLVLGLAQQLRGAHYMSHTFWTAWCCWVVACACDSAARRDAPSPTVVPRHET
                     C"
     gene            14847..15401
                     /gene="efp"
                     /locus_tag="VGA59_14845"
     CDS             14847..15401
                     /gene="efp"
                     /locus_tag="VGA59_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX9720994.1"
                     /translation="MKIAQEIRAGNVIMHGKDPMVVLKTEYARGGRNAATVRMKLKSL
                     LNNFGTEAVFKADDKMDQIMLDKKDCTYSYFADPMYVFMDAEYNQYEVEKENMGDALN
                     YLEDGMPAEVVFYEGKALSVELPTSLEREITWTEPAVKGDTSGKVLKPAKIATGFEIG
                     VPIFVAQGDKVEIDTRTGEYRRRV"
     gene            15476..16279
                     /locus_tag="VGA59_14850"
     CDS             15476..16279
                     /locus_tag="VGA59_14850"
                     /inference="COORDINATES: protein motif:HMM:NF023354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent oxidative N-demethylase subunit
                     alpha family protein"
                     /protein_id="HEX9720995.1"
                     /translation="MDFDFSQINVPFRMQPGLRRIDARALRLTPLHAASRLYAEKQAV
                     FAAGRSRHTVADFDPGPALEAIAALGGASGIPPAGASAAPVELAFEEDFAVLDGATGT
                     LPWLCVCVPSHWAPEDKLGLDFAAVHAPVADNAALVAASRQLVSLVTAGDCWERFVWT
                     ISPSAWHDQHPRRHTRQPWPAPADADDFAGRCWLRAERQTFFPVGRGTRQAVFAIHVM
                     LQRLAEAVEAPGLPRRLHDSLASMSQAVLDYKNLGPAREPLLRWLQSRT"
     gene            complement(16289..16978)
                     /locus_tag="VGA59_14855"
     CDS             complement(16289..16978)
                     /locus_tag="VGA59_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9720996.1"
                     /translation="MDETVYHVLLEGRTVGPYDRRTIVGMRIKKTLTSDHVLISTGGA
                     QLTVAELIGQRPARQFNPDRSGSLSVVRATYAASLLEVDGRGMEIPRFKGEVQARVQG
                     DVLRIAGRFRQRLRWQEDRVKIPLKDVVHARVNGSQVNLWLRNAQESRLQRISLELFT
                     PETAGELIDWLPVATPFREPAAPAQGPTPQDDVLALPEESASSGLWVAVAGATLVVVL
                     MLMVLLLRRGY"
     gene            17052..17915
                     /locus_tag="VGA59_14860"
     CDS             17052..17915
                     /locus_tag="VGA59_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX9720997.1"
                     /translation="MASTRNLHDRRLADAWATLQAHSSAGLPLDPDASRLAFADPEFL
                     LRRETRGIRFQLELLKPDLEQQAMGIENTIVVFGSARFRSEEDAAALIAAAEADGDEA
                     QIRRARAVARNAHYYEKARAFGKLVARYSAGKKPEDMLFICTGGGPGIMQAANRGAYE
                     GDGISVGLSIALPMEESANAYVTPALSFKFHYFALRKMHFMMRAKALVAFPGGFGTLD
                     ELFEVITLVQTRKARQVPIVLFGTDYWKRLVNFQVLIDEGVISAVDLDLFQYADEPDA
                     AWDIIKAFYRL"
     gene            complement(17935..18162)
                     /locus_tag="VGA59_14865"
                     /pseudo
     CDS             complement(17935..18162)
                     /locus_tag="VGA59_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(18230..19561)
                     /gene="rmuC"
                     /locus_tag="VGA59_14870"
     CDS             complement(18230..19561)
                     /gene="rmuC"
                     /locus_tag="VGA59_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX9720998.1"
                     /translation="MELWTLVALAIANLVLVVALFVRKPPVNEAGRAELLAANERMER
                     ELRREISESSRGARTELTHTLATFQDALVKQGAEATRTQNTQIDTFGQQLTLLQKTLS
                     DTLSLQLQNLSESNARRMAEVRGTLEAQLAMLQQTNTAKLEEMRMTVDEKLQTTLETR
                     LGESFKQVADRLEQVHKGLGEMQTLAQGVGDLQRVLTNVKTRGMFGEVQLEALLEQVL
                     THEQYAKQVETKPRSNQRVDFAIRFPGRTDGTPVWLPVDAKFPRDDYERLLDAQDRAD
                     PAAADIAARALEARIRTEAKSISECYLCPPQTTDFAILFLPIESLYAEVLRRPGLMDS
                     LQRDYRVTLAGPTTLLAMLNSLHMGFRTLALEQQASEVWKVLGAVKTEFERYGSWVEK
                     IREQVRKASDTLETAQTRTNQMRRALKVVEALPEAQAQALLPPVEGEEEPA"
     gene            complement(19570..>20431)
                     /locus_tag="VGA59_14875"
     CDS             complement(19570..>20431)
                     /locus_tag="VGA59_14875"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX9720999.1"
                     /translation="ARKLHDKLGAFTTGGDRIDADVLAAAPQLKICANMAVGYNNFDL
                     PAMTARKVLATNTPDVLTETTADFGFALLMAAARRVTESEHYLRAGKWQRWSYDMFAG
                     SDVHGSMLGILGMGRIGQGIARRGAHGFGMKVIYHNRSRLAPELEAQCKARYVGKEEL
                     LRTADHLVLVLPYSPASHHAIGAAELALMKPTANLINIARGGIVDDAALATALREKRI
                     AAAGLDVYEGEPKVHPDLLTVPNVVLTPHIASATVPTRKAMADLAADNLIGFLVHGKP
                     LTPLNPEVLN"
BASE COUNT         3395 a         6924 c         6675 g         3437 t
ORIGIN      
        1 taggccaggt tttcggccga ggcccaccac tgcgcatact gctggcgctc ctgcctcgcg
       61 acgtcctcgg tcggagcctc acggggcagc accttgttca ccttgaccac cgcgtaaccc
      121 tgatcgccca ggtcgacgcc cgcgaggacg ggcaacgcgg cggggtcggc cctcagcgcg
      181 gcctcgacca ctgcctgcgg ctgcttctga gtttcctgcc gcgagaccgc gatggccgcc
      241 ggcaggccgg cacccgcggg attcgccttc caggccgcca gcttctccat gccttccttc
      301 ttcgccagct cggccgcgcg cgcagccacc aggcgctcgc gtacgcgctc cttcacttcg
      361 gccagcggga gcgtgcgtgc cggcgtgtac tgggtgacgc gtccggccac cagggtattg
      421 ggggctatct ccacggcttc cgtgttgcgc ttcttttgcg tgctgtcggg cgagaacagg
      481 gccgtgagga acttggggtt ggccagggcg ccgctggctc ccgggctcgg agtgcgagtc
      541 acgttgctgg ccgtcctgat ttccagcttg aggcggtccg ccaccggctt caggctgtcg
      601 gcctgctcgt acacaccatt gctgaaggtg tcggccgctt cggcgtactt gcgctgggcc
      661 tgctgtttct tcaggtcggc ctccagctcc ggtctcatgt cctcgaagct gcgctgcttg
      721 ggcgccttga tatcggtgac catgatgatg tggtagccga attcggtttc caccaggccg
      781 ctgagctcgc ctttcttgag cgcgaaggtc gcgtcctcga agggcttggt catggcgccg
      841 cggccgaaga aatccagatc cccgccgttc gcggcggacc cgggatcctg cgagtctttc
      901 ctggcgacct cggcgaaagt gccgggcgac ttcctgacgg cagccagcag ttcctccgcc
      961 cgcgctttgg ccttttcgcg ctcgggcgcc ggggcgccct tgggcaccgc gaccaggatg
     1021 tggctggcgc gccgctcctc ctgccccgaa agccgtcccg cgttctgctc gtaataggtc
     1081 ttcagatcct gctcgttgac agtcatccct ttcttgatcg tgtccaggtc gagcaccatg
     1141 tactcgatgc cggcctgctc cggcgcctgg aacagcgctg cattggcctt gtagtaggcc
     1201 tccagttcgg catcggtcgg tttgacacgg ccggcatagt cgggcgcggc aaagcgcgcc
     1261 acctgcactt cgcgcttttc gaagagcgcg ttgagagaga gattcgcctg gcccggcgtc
     1321 gccaggctgg tgccgcccac gccgcccagc acctggcgcg cggacaggtc ggcccggacg
     1381 ctggcttcga acatctctgg cgtcattcct tgcgaactca cgagctgccg atagcgggcc
     1441 atgtccagct tgccgtcggg cccgcgcaag gaggcgatca gttcgttctg ctgcagctcg
     1501 cgcgccaggc gctggtcgcc ggtgacgagc ttggatttcg ccgcggcggc ggccagcacg
     1561 cggtcgcgca ccatgcgctc cagggtcgcg tagcgcgcgg cgggtgaatc cagcaacttt
     1621 ggatcgagcg tgggcatgga ctggcgaagg cgttcgacct cctgcttgtg cgccgcatcc
     1681 cagtcgccct ggcggatctc ctcgccgtcc accgtggcga cggcctcgcc tttatcctgg
     1741 aagcggtcgt agccactgat gcccacgagc acgaatgacg ggacgatcag caggaacagc
     1801 acgagctgca tgatcttgag gtgcttgcga acgaattcga acatgctctg actcacaacg
     1861 tttaagacac acttcgggcg gcgccgccga cgggctgcgc cgacaattcg cactgcaatt
     1921 gtcatgcgca aacaaaaggc gaacaactgt tcgccttcgg ctggttggtg gtgggtgctg
     1981 acgggatcga accgccgacc tacgccttgt aagggcgccg ctctaccagc tgagctaagc
     2041 accccaccgg aaaaaacgca ccgatcagtt cagcgcatcc ttgagcgctt tgccgggtcg
     2101 gaacttggga accttggctg ccttgatttt aatcgctgcg ccggtccggg ggttacgtcc
     2161 gctgcgggcg gcccgcttgc ccaccgcgaa ggtgccgaat cccaccagcg aaaccgcacc
     2221 gccctttttt agcgtggtct tgacggcgcc gatcatcgat tccagcgcgc gcgtggcggc
     2281 ggccttcgag atatcggcat gctttgcgat gtgctcaatc agttcggtct tgttcacaag
     2341 gggtccctcg tacagagaat tagttgatca ggatgtctct tcaaaaaaat attgtctccc
     2401 gatcctgcgg ccaccgtcag gtgccttgag gcgaggatgc gggagacttc ttgcgcggct
     2461 tcagccaccg actaattaga gcgacggacc cacaccgtgt caatacaacg ggcgccttta
     2521 caacagcgcg gaggcggatt ctgcccgctt ttggctagat ttcatgcggg ctccgggccg
     2581 atggcatgcc gcacggggcc aattttcacg agcagaaatg gctcgacccg ttacagggcc
     2641 gacgcaatgg cgcgccccag gtcgctcgta gtcgccgaac cgcccaagtc cgccgtgcgc
     2701 ggcgcaccgc ttcccggctg aagaactctt tcgatggccg acagcaccgc atcatgcgcc
     2761 tgccggtacc cgaggaagtc gagcatcatc gcaccgcacc agatctggcc gatcggattg
     2821 gcgatgttgc gcccggcgat gtccggcgcg gacccgtgca ccggctcgaa cagcgacgga
     2881 aacttgcgat cgggattgag gttggcgctc ggtgcgatgc cgatggttcc ggtgcacgcc
     2941 ggccccaggt cgctgaggat gtcgccgaac aggttgctcg ccaccaccac gtcgaaaaaa
     3001 tcgggccgct gcacgaaatg ggcggtgagg atgtcgatgt ggaacttgtc gacgttcacg
     3061 cccggatagg ccttcgccat ttcggcgacg cgctcgtccc aaaaaggcat ggtgatcgcg
     3121 atgccattgg acttggtggc gctcgtgagg tgcttcttcg gacgcgagcg cgccagctcg
     3181 aacgcgaact tcagcacccg gtccacgccc acgcgggaca tcaccgtctc ctgcatgacg
     3241 atctcgcgct cggtgcccgg atacatgcgc ccgccgatgc tggagtattc gccttcggtg
     3301 ttttcccgga cgatgtacat gtcgatctcg cccggttggc gcgcgctgcc gtcgcggcgc
     3361 accaccggag cgatgatgcc cggcatcagg cgggccggcc gcaaattgac atactggtcg
     3421 aactcgcgcc ggaacagcag caatgaaccc caaagcgaga catgatcggg aatcttttcg
     3481 ggccagccga cggctccgaa atagatcgcg tcatggccgc cgatccggtc cttccagtcg
     3541 tcgggcagca tcttgccgtg cttctcgcag taatcccaac tggaaaaatc gaagtggtcg
     3601 aacgcgaggt cgatgccgaa cctgcctgcc gccgcctcca tcacccggat gccctcgggc
     3661 atcacttcct tgccgatgcc gtcgccggca atcactgcga ttcgttttct ggccatttca
     3721 aggtacccct gtgtagattc attcaatgac ttgcgctgaa aaactgcaag gcttcctcca
     3781 gcaaaagcgc cggcgcctct tccgcgatgt agtggccgca gggcaagctg cggccggtca
     3841 cctgtcgtgc ccgctcgcgc cacaacccca gcacgtcgaa gcagcggccg acggcgccat
     3901 gttcgcccca cagcacgcgc agcggctgct ccagccggcg gtcggcggcc acgtcggcgc
     3961 gatcgtgtgc catgtcgatc ccggcactgg cacggtaatc cgcgcagatc ccctgagccg
     4021 tgcccgcgat gccggcacaa cgctcgtact cggccagggc ctcgggcgca aacggcgcca
     4081 gcccggcatg gcggctcccc atcacgctgc gcacgtagcg gacaggatcg gattcgatca
     4141 gggcttcggg cagcggtggc ggctggacca ggaagaacca gtgccagtag gcttgcgcga
     4201 acgccagcga ggtccgctcg tacatcgcga gtgtcggcgc gatgtccagc agcatcaggc
     4261 gatcgaccgc tttcgggtgg tcggcggcca gacgatgggc cacccgggca ccgcgatcat
     4321 gcgccagtac cttgaagcgc gcgaacccct gttcctgcat cacagccagc gcgtccagtg
     4381 ccatctcgcg cttggagtag gcacagctgc cctcccccgc atcgggccgc cccgaatcgc
     4441 cgtaaccgcg caggtccatc atcaccaagc tgtatgcccg ggcgagcccg ggcgccacgc
     4501 gatgccacat cgcatgggtc tgcggatggc cgtgcaacag gagcaggggc tcgccctgcc
     4561 cgccgcggcg tccgtgcagg cgcacgccgt cgcgtaccac ctcgaagctc tcgaatgacg
     4621 ccagcatgaa gccattctgg cattgcgccg catgcctgag caagcaataa tccggcaatt
     4681 gattatttcc agatcggcaa caattgaacg gcctgctcga ccgcggcgat cttgaactgc
     4741 tgcttgccgt gcgcgcgcac ggcagcttgg cggcgacggc cgcggctgct ggcgtcgtgc
     4801 cctccgtcgt gaccaagaag ctggccgcgc tggaggtgcg gctgggcttt cggctattcc
     4861 agcgcaccac gcggcgcatc gtcgccaccg ccgaaggcga ggcgctgtgc gagcgcgcgg
     4921 cccagctggt gcaagccttc tcccagctcg agacggacct gcgtgagcgc catagcgaac
     4981 cgtcggggct gatccggctg acggccacgt tcggtttcgg ccgcctctgg ctcggaccgg
     5041 cgctggccga tttccaggcg cgccacccgc tgctgcgcgt ccagctccag ctcaccgagc
     5101 agttgcccaa cctcgcggtg gaaggttacg acggcgccgt gtggctgtgg tcagtgcagg
     5161 gccgccgcgc caccgagtgg gtgggccggc gactcgcgcg caaccagcgg gtgctggtcg
     5221 ccgcccccca gtacctgcgg cagcgcggcg cgccccagac gcccgccgac ctggcgcagc
     5281 acgactgcct gatcgtgcgc gagagtgacc agcgcttcga catctgggcg ctgcgcaaag
     5341 agcgcgacaa agtcgaggtg cgggcgcgtg tgcaagggcc cttgtccagc aattcaggcg
     5401 agctcgtgcg cgattggtgc ctggccggcc gcggcatcat gctgcgcagc ctgtgggata
     5461 tcgcgccgca actggcctcg ggccagctgg ttcgcgtgtt gcccggctat gcgatgttcg
     5521 acgccgacat tcattggctc gcgccgcatc gcgcccagac gcccaagcgc gtgcgcctgc
     5581 tgatcgattt cctggccgag cgcttccggg gcgaaccgtg gaaactcaag cccgctgcgg
     5641 cgaaggctga cgcaccacca ggctgacgcc cagcgccgcg agcgcgatac ccgtgatggt
     5701 gagcagcgtg atcgcctcgc cgaacagcag ccatgccaac atggccgtgc aaggcggcac
     5761 caggtacatg aggctcgaca ccgccgtggc cgcgccgcgg tggatcagca ggtacagcag
     5821 cgagctgccg cctaaagtca gcgcgagcac cgaccatgcc atggcgccgg ccaactcgcc
     5881 attccagcgc atgctttcgg tttcgagcac cgccagcggc agggtgacgg cgaaagcggc
     5941 gcaaagctgt accaggctgg cggtgcggac atcgcaaggc gccaggaatc gcttctggta
     6001 gatcgtgccg gccgtgatgc tgagcaaggc gcccaccgcc aacaggaagt tggcgccgct
     6061 gatctcgccc acgcccagct tctgccatac gaccagcgcc agcccgccga aaccaagcgc
     6121 caggccggcc cactggcgcg ccgacaccac acccccgcgc gacgacaccc ataccgcggt
     6181 cagcaccggc tgcagcccca ccagcagcgc cgccaggcct gcccccatgc ccagcttgac
     6241 cgccgcccag acgccgccca gatagccggc gtgcatcaag ataccggtca ccgccaggtg
     6301 cttcacttgc ccgcgctcgc gcggcagcaa tgcgacccca gccgccgtgg cccacgcaac
     6361 gaagcaggca accgacagga aatagcgcag cgccaggaat ttcatcggcg gcgcatgcgg
     6421 catgccgtag cgggcgacga tgaagccggt gctccagatc acgacgaaga tggcgggcat
     6481 ggcacgcacc agcgcgtcct gccgggacaa agaactcatc tggctatttc acccgcttgc
     6541 ggatttccgg cagcgctttc tggaggtaat agaccatgga ccacacggtc aggatcgccg
     6601 cgatccatat cagccaggtt ccccacaggc cggtgtctat gacgccgaac agcaagccgt
     6661 cgaacagcag gaacgggatc gcgatcatct gaaccacggt cttgagcttg cccagcatgt
     6721 gcaccgccac gcttttggaa gccccgatct gcgccatcca ctcgcgcagc gccgaaatcg
     6781 cgatctcgcg gccgatgatg atcaacgcca cgaacacgtc ggcccgcttc aggtgcacga
     6841 gcaccagcag cgaggcacac accaggaact tgtccgccac gggatccagg aaggcgccga
     6901 aggacgacat ctggttgagc cttctggcaa ggtagccgtc cagccagtcg gtgagcgcga
     6961 aaaccacgaa catggcggtg gcgacgagat tttgcagggc cggatccagc cccaggtaga
     7021 acacgccgat gatcagcggg atcgcgacga tgcgcgtcca ggtcaggatc gtggggatgg
     7081 tgaggaacac ccctcgattc tggcatgagc ggcgtcgagc gttgggcgat gggcgtcggg
     7141 cgtgaattca cgcccgccgc ccatcgctcc acctcagtga agcgcgcggt agatctcttc
     7201 ggccagttcc ctggagatgc cttcaacgct ggcaatgtcg tccacaccgg cggcggccac
     7261 gccgcgcacc ccgccgaaac gctgcaacag ccgggcgcgt ttcttcgcgc ctatcccggg
     7321 gatgtcctcg atctggctgc cacccactcg caccctcgcc cttcgcgccc tcattccggt
     7381 gatggcaaag cgatgggcct cgtcgcggat ctgcgccacc agcatcagcg cggccgagtc
     7441 cttgcccagg taaaccttgt cgcggccgtc ggcgaacacc agctcctcca gtcccacctt
     7501 gcggccttcg cccttttcga ccccgatgat caccgcgagg tccaggccga gttcggcaaa
     7561 aacctcacgc gccatgctca cctgcccctt gccgccatcg accagcacca ggtcgggcag
     7621 ccggcccgcc ccgcctgcgc ccgctgcccc accggcgccg tccggcgggg tagcgtcggg
     7681 cgagtcgcgt ccggcttcgg ccagcttgct gtagcggcgc agcagcacct gccgcatcgc
     7741 ggcgtaatcg tcgcccggcg tgataccgcc gatgttgtag cgccggtact cggcgttctg
     7801 catcttgtgg tggtggaaca cgacacacga cgcttgcgtc gcctcgcccg ctgtgtggga
     7861 gatgtcgaag cactcgatgc gcaagctgtc caggttatcc ggcgcgaggt ccagcgcctc
     7921 caccagggcg cgcgtgcgcg cctgctgcga accttcctcg gccaacagcc gcgccagctg
     7981 gatggcggca ttcttctgcg ccatctccag ccacaggcgc cgctgctcgc gcggattgtg
     8041 cacggcgctg atcttcaggc ccgactgctc gcttagcgcg tcgatcagct cgcggccgac
     8101 cggctcgctg gtgatcagcg ccggcggtac gggcacgccg atgtagtgct gggcgataaa
     8161 ggcttcgagt atctgcactt ccacgggcat cgggggcgcg acctccagcc cctcgtcgac
     8221 ccggctgtcg aagtcctgca ccgccgcggc atcctcgatg tggctgggga agtaaggccg
     8281 gtcgccaaga tggcggccgc cgcgcaccat ggcgaggttc acgcacgcct tgccgccctg
     8341 cactttgacg gcgaggatgt ccacgtcctt gtcggaaatg gtctccacgg actgctggtg
     8401 cagcaccttg gacagcgcgc cgatctggtt gcgcaactcg gccgcctgtt cgaactcgag
     8461 cttttgcgcg tgctgcatca tgcgggcctc cagcgccgcc agcaccgcct gcgtttcgcc
     8521 gcgcaggaaa gcctcggcgt tatgcacgtc gtgccggtag tcctgcggcg aaaccaggtt
     8581 gacgcagggc cccgaacagc gtttgatctg gtagagcagg cagggccggg tgcggttggc
     8641 gaagacagtg tcttcgcagg tgcgcagccg gaacaccttc tggatcagct ggatcgattc
     8701 cttcacggcc caggcgctcg ggtacgggcc gaaataccgg tgtttccgat cgacggcgcc
     8761 ccggtaatag gccacgcggg gaaacgttgc cgccgacacc gccgccgtct cgctcaaccc
     8821 gcctttctcc ggaccggaaa ttttcaggta ggggtagctc ttgtcgtccc tgaagaggat
     8881 gttgtagcgg gggttgagcg tcttgatcag gttgttttcc agcagcagcg cctcggcctc
     8941 cgagcgcacc acggtggttt caaggcgcgc gatcttgccg atcatgtggc cgatgcgcgt
     9001 gccgccgtgg ttcttctgga agtagctgca cacgcgcttt ttcaggttgc gcgccttgcc
     9061 cacgtacagg acggtgccct gcgcatcgaa atagcgatag acgcccggca gcgcgggcag
     9121 cgccgcgacc tgcgccagca gctgatcgga atgaacctcg gacatacaga cgctattgtg
     9181 gacaatccac ggaaacccat cgaccccacc atgctctggg acatcttctg caaggtcatc
     9241 gacaaccacg gcgacatcgg cgtgtgctgg cgcctgtcga cccagctggc tgggcgcggc
     9301 gagcaggtgc ggctctgggt cgacgatggc ttggcgctgg gctggatggc gccccacgga
     9361 tgtgccggtg tgcaggtcct gccttgggcc gccgggtcgc agccgcggga acccggtgcc
     9421 gtggtgatcg aggccttcgg ctgcgaactg gcgccggcgt tccaggcgac gctcgcggcc
     9481 aggacccgga aggagggaca tcaaccggtg tggctgaacc tggagtacct caccgccgaa
     9541 tccttcgccg aacgcaacca tgggttgccg tcgcccgtcc tcgccgggcc ggcggcggga
     9601 ttgaccaagt attttttcta ccccggtttc acaccgggaa cgggcggcct gctgcgcgaa
     9661 cccggcctgc tggagcggca ggagcggttc gaccgcgagg cctggctgga ccgccacggc
     9721 atccacgtgg gcaaaggctg cgttctggtg agcctgttcg gctacgagcc ggccgcgctg
     9781 gatcagctgc tggcgcactg ggcgcgcggc cccggccgca cccatctgct ggtgacggcg
     9841 ggacgcgccg cagcggcggt ccacgcggcg gtcgcaagaa aagcgcagtc ggagccgcag
     9901 tggaaccggt ccgcggcact gacgacgtcc tggctgccct tgctgaccca gctggacttc
     9961 gaccacctgc tctgggcctg cgacctgaat ttcgtgcgcg gcgaggattc gctggtgcgg
    10021 gcgctgtggg ccggcaagcc gctggtctgg cagatctatc cgcagcatga cgatgcgcac
    10081 cacgccaagc tcatggcttt cctcgactgg ctcgaagctc ccgccctcct gcgcgagttc
    10141 cacctggcct ggaacggtgt cggcggcgcg ctgcccgctg cctcgccgga cacctggggc
    10201 gagtgcgtgc agcggggccg acttcggctg gcggcgcagg acgacctggc gacgcagctg
    10261 atgcgttttg tcgcttctcc tggacgcgct ccggcatgag gctgctgctc gttgaagacg
    10321 acgccatgat aggccgcgcg gtacgccagg ggttgggcga tgccggtttc acggtggact
    10381 gggcgaccga cggccgcgcc gcggaactcg cgctcgccaa tggcgtgtat gacctggcgg
    10441 tgctcgacct gggcctgccg ggcaaggacg ggatgacggt gctgcgagaa ctgcgtgccc
    10501 ggcgcgaccc gctgccggtg ctgatcgtca cggcgcgcga tgccgttccc gaccgcatcg
    10561 aaggcctcaa tgcgggtgcc gacgactatg tgctcaagcc gttcgatctg gacgaattgg
    10621 tggcacgagt gcgcgccctg ctgcggcggc aagccggtgg cgcggtgccg gtgctgcaag
    10681 caggggagct gacgctggac accgtgcgcc gtgaagtttt tctggaaggc agggagatcc
    10741 ggctgtcggc gcgcgagttc gcgttgctgg aagcgttgat gcggcggcca ggcgccgtgc
    10801 tgtcccggga acggctggag gacgccgtgt acggctgggg tgaggaagtg ggcagcaatg
    10861 ccgtggaggt ccatttgcac aacctgcgcc gcaagctcgg cgccgagacc atccttaatg
    10921 tgcgcggcgt cggctacaag gtgatcgaca agtgaccgcg tcgatccacc gccgggttct
    10981 cataggggcg ttcagtgcct tggcgcttgg ggccggcctg ctggtgggcg gttcctggtg
    11041 gctgctggcg caggagatga acgaggtttt cgaagacaac ttgaagcagg tcgctctggc
    11101 ggtggcgagc catcatgacg ccggtgccat gacaggccca cctcggctcg cgcaacagct
    11161 gccccgggtg tacgaggagt acgggaagtt cgaattcgtg acgcaagcct ggtcacgcga
    11221 gggaaagctc ctgcacagat ccgacgaggg ggcagcgctg ccatttctgt cgcgcagtgg
    11281 cctgagcacc gtcgcggtcg gcggcgaagc atggcatctg tacacgatcg tgctggaaga
    11341 tggcgtcgtg caggccgcgc agcgcgccag cgaaaggcag acgctggccc gggaaaccgc
    11401 ttcggcgctc atcctgcccg ctcttgcgat gctggccgcg atcgccggcc tgctggcgct
    11461 ggccctgcgg cgcgggcttg cgccgctgtc gagcgctgcc ggcgaggtca ccacgcgcag
    11521 cgtggaggcc ctgcatccga ttgcgctggc ttcgcagccg gccgaactgc acttgctggt
    11581 gggcgcgatc aacgacctga tggccaggct gggcagcgcg ttggcgctgc agcgtaattt
    11641 cctcgccgac gccgcgcacg agctacgcac gccgatcgcg gcgctgcgcc tgcagctgca
    11701 attgctggat cgggcacgag gggaggatca acgcgccgcc gctcaggacg agttgcgcgc
    11761 gggaatcgcc cgggcgcagc acctggtcga gcagctgctt cagttgtccc ggttgggtcc
    11821 ggaaacgccg gcgctcaagc aggagatggt ggacctggcg gaacttgcgc gagccgccgt
    11881 ggcgcggttc agcgcgcgag ccgagcagca aaggatcgac ctgggggcaa tcgcggcgaa
    11941 cgaagccatg atccagggcg atgcgcaaca cctcacgatc ctggtcaaca acctggtgga
    12001 caacgcgctg cgccacacgc cggcgggcgg caaggtcgac gtctgcgccg aaccgcggca
    12061 ggggcaaccg tgcctgatcg tgacggacag cgggcccggc attccggctg cggaacgtga
    12121 acgcgtgttc gaccgtttct atcgttcggc ggatgcgcca ccctcgacgg gaagcggcct
    12181 ggggcttgcg atcgtgcgcg cagtggcaca acggcatggc gccgaagtca ccctcgggga
    12241 ggcgcccggc ggcggcctct ccgtcagtgt gctgtttccc tcaccctagg tccactcggt
    12301 ggactagttt cgcggcaagg tgcgaaaaga tccaggcggc ggtcgtaggc tgccgtttca
    12361 acgtccagca cgccgagcag cgagtgaaac aggttgtcat ggcttgcggg ctcgacggcg
    12421 cgcccgcgca tgcattcctg gtcgataccg aaaccggcgc ggaaagccgg cgacagccaa
    12481 accacgaacg gaacgtcctt ctgcacgtcc ggcgcgattg cgtacggcat gccgtgcaga
    12541 tacagccccc cctcccccag cgattcgcca tgatccgaca catacagcat cgcggtgtca
    12601 tgggtctcct gcgcccgctc caggaagtcg atcaccttgc cgagaaagaa gtcggtgtac
    12661 agcagtgagt cgtcgtacgc attcacgatc tccgccgggc tgcagcggcg caagtcttcg
    12721 ctctcacagg ccggctggaa gcgcttgagg gccgccggat aacgcttgaa gtacgccggc
    12781 ccgtggctgc ccagctggtg catcacgaca aacaggttgc cccggctgtc gcgggcgatg
    12841 ccctccatgc cttgcaacag gacttcgtca aggcactggc cttgcgcgca caagccttcg
    12901 gtcgatgccc ggtcgagctg ttcctgcgca agcccatcgc acacaccctt gcagccggac
    12961 tgattgtccc gccacagcac cttgaagccg gcccgcgcca gtacatgcag cacggattcg
    13021 tgggtcagga tttcatcctc gtcgtaatgt cgccggccga attgggagaa catgcacggc
    13081 aacgacacct cggtcgaggt cccgcaggcc cggacatggg gaaagttgat gactccgtgc
    13141 cgggccagct gcgggttggt ttcgcggccg tacccgttga gggagaagtt cttggcccgg
    13201 gcggtttccc ctacgaccag aacgaacagc gttgggcggg tgcgaccgtc ccacgcggcg
    13261 cccatccgcg cgtcggcgcc caccggcagg cggggtcgat tcggcatccg ggcccggccc
    13321 caggcgttgg ctgccgccgc cgcaacgagg ttccccgggg tgatcagata gcgcacttcc
    13381 ttgtgattgc gcatcagcga ggcgaagtcg gcaaacacgg gcagcaggct cgcaattccg
    13441 attgccaacg cccctgatac ccatgccagc cgcaccatga ctgcgcaccc gaaggttcgg
    13501 cgcttgagct gtggccacca caccagcagc gacggcagca ccccccacgc gagcaggtgc
    13561 agcagcaggc ccgagccgag cagttcggtc gcctccttgt agtgcgtggc gagcacattg
    13621 cgcaacatcg cgcgatcgac atagatggag taacggtcca tgtagtagga cgcacccgcc
    13681 gagaccagca gcaacgccgt gagcagtggc ttgacggtgt gcctgcctgc gacggcggcg
    13741 gcggccgcga ggtagaacgc ggtgaatgcc gcaagcagcg accccgcgaa gccccaggtg
    13801 gcaagttcgc cccatgagcg gcccgccaat gccgcgcgcc agaaggggcc gttgccgatc
    13861 gccatcagaa agacagccac cccgagcacg agcatttccg gatgaacgga cgggcggccc
    13921 cgcgcggtgg gcgaggcctc aacaggtctc atgccgagga acgacggttg gggaaggtgc
    13981 gtccctgcgc gcggccgaat cacaggcgca ggcgacgacc cagcaacacc aggccgtcca
    14041 gaaggtatgg ctcatgtagt gggcgccgcg cagctgctgc gccaacccga gcaccaggcc
    14101 ggccgccagc accgctgcca ggcccagcat ggcccggcgc cgcgagacct gccgcagcgc
    14161 gaaatagccg ccgatgaacg cgaaggccgc cgacgcgtgg ccggccggaa agcagcgccc
    14221 cggacccgcg tcggacttgc cccaggccca atgggacagg tagctggctg aacccccgaa
    14281 ctccgaaagg tcccagggac aactggtctg gctcgaatac ttgagcgcgc tgatgagaac
    14341 cagcgagagc agcgcgctcg ccagccattg cagccgcgcg gggcgggcaa gctgccgaag
    14401 ccatccggtc ggccaccaca cccccaccag tagccacagg gccatgagcc agcccagctg
    14461 gcgcccgccc tggtgcagca cacccgcgag cgcccagtgt tcgcgcaggc tgaagccgcc
    14521 gccgcccgcc atcagccggg ccaggggaag atccagcccg gcggcatccc aggccagcag
    14581 gactgctaac gaggccacgg cccagatcca gggttttggg aacgcggtcg tgacgccttg
    14641 ctggaaaatt cgcattcggc cgagtctgcg agggcgacac ttaaggcagc ctgaaacgcg
    14701 tttcgccccg gctgatcggc tcacattcga aagacgctaa aataaagggc tttgcgcgtt
    14761 ggccgtgtcc cggccaagcc tgtgcaaccc gccgcagtcc gcgcgccctc ccgggcccgc
    14821 cgcggcctac tttctcgcaa actgctatga aaatcgctca agaaattcgc gccggcaatg
    14881 tgatcatgca cggaaaggac cccatggtcg tgctcaagac ggagtacgcc cggggcgggc
    14941 gcaacgccgc cacggtgcgc atgaagctca agagcctgct gaacaatttc ggcaccgaag
    15001 ccgtgttcaa ggccgacgac aagatggacc agatcatgct ggacaagaag gattgcacct
    15061 actcctactt tgccgacccg atgtacgtgt tcatggacgc cgagtacaac cagtacgagg
    15121 tcgagaagga aaacatgggc gacgccctga actacctcga ggacggcatg ccggccgagg
    15181 tggtgttcta cgagggcaag gccctgtcgg tggaactgcc gaccagcctc gagcgcgaga
    15241 tcacctggac ggagccggcc gtcaagggcg acacctcggg caaagtgctc aaacccgcca
    15301 aaatcgccac cggcttcgaa atcggtgtgc cgattttcgt ggcgcagggc gacaaggtgg
    15361 aaatcgatac ccgcaccggc gaataccgca ggcgcgtcta aggccctaaa gcaggcctca
    15421 ggccgcgcgg ctacgatccc aggctccgca aggagccttt tttcagcctc gccgcatgga
    15481 cttcgacttc agccagatca acgtgccgtt ccggatgcag cccggcctgc ggcgcatcga
    15541 cgcgcgggcg ctgcgcctga cgccgctgca cgccgccagc cggctgtacg ccgaaaagca
    15601 ggcggtgttc gcggccggac ggtcgcgcca caccgtggcc gatttcgatc cggggcccgc
    15661 gctggaagca attgccgcgc ttggcggcgc gtcgggaatc ccgccagccg gggcaagcgc
    15721 ggcaccggtg gagctcgcgt tcgaggaaga ctttgcggtg ctcgacggcg cgaccggcac
    15781 cctgccctgg ttgtgtgtct gcgtgccatc ccattgggct cccgaagaca agctggggct
    15841 ggatttcgcg gctgtgcacg cacccgtcgc agacaacgcc gcattggtcg ccgcttcgcg
    15901 gcaactggtg tcgctggtga cagccgggga ttgctgggag cgcttcgtct ggaccatcag
    15961 cccctcggcg tggcatgacc agcacccgcg ccggcacacg cggcaaccct ggccggcccc
    16021 ggccgatgcc gatgacttcg ccggccggtg ctggttgcgc gccgagcgcc agaccttctt
    16081 cccggtcggc cgcggcacgc gccaggccgt gttcgccatc cacgtcatgc tgcaacggct
    16141 ggccgaagcc gtcgaggcgc cgggtctgcc tcgccggctg catgactcgc tggcatcgat
    16201 gtcgcaggcg gtgctcgact acaagaacct cggcccggcg cgcgaaccct tgttgcgctg
    16261 gctgcaatcc agaacctgac tgatcgcttc aatagccgcg ccgcaacaac agcaccatca
    16321 gcatcagcac caccaccagc gtcgccccgg ctaccgccac ccacagcccg ctggacgccg
    16381 actcctcagg caatgccagg acgtcgtcct gcggcgtcgg cccctgggcg ggcgctgccg
    16441 gttcgcgaaa cggggtggcc acgggcagcc agtcgatcag ctctcccgcg gtctcgggcg
    16501 tgaacagctc gagcgagatc cgctgcaacc tggactcctg cgcattgcgc aaccacaggt
    16561 taacctgaga cccattgacc cgggcatgaa ccacatcctt gagggggatc ttgacgcggt
    16621 cttcctgcca gcgcaggcgt tggcggaacc ggcccgcgat ccgcagcacg tcgccctgta
    16681 ccctggcctg gacctcgccc ttgaacctgg gaatttccat gcctcgccca tcgacctcca
    16741 gcaggctggc ggcgtaggtg gcccgcacca cggacaaact gccgctgcgg tcggggttga
    16801 actgccgggc cggtcgttgt cctatcagtt cggccacggt cagctgcgcg ccacccgtgc
    16861 tgataagcac atggtcgctc gtcaacgtct ttttgatcct catccccacg atcgtcctgc
    16921 gatcgtacgg cccgacggta cgcccctcga gcaggacgtg gtaaaccgtt tcgtccaact
    16981 ctgtccccag aaagtattgc ttgaacgcga gcataccgga taacgcaccc gattggccgc
    17041 aaaatcgggg catggcttcc acccgcaacc ttcacgaccg gcgcctggcc gatgcctggg
    17101 caaccctgca ggcccactcg agcgcgggcc tgccgctgga ccccgacgcc agccgcctgg
    17161 ctttcgccga ccccgagttc ctgctgcgcc gcgaaacgcg cggcattcgc ttccagctcg
    17221 aactgctcaa gcccgacctg gagcagcagg ccatgggcat cgagaacacc atcgtcgtgt
    17281 tcggcagcgc ccgttttcgc agcgaagagg atgccgccgc cctgatcgcg gcggccgagg
    17341 ccgacggcga tgaggcgcag atccggcggg cccgggcggt cgcgcgaaat gcccactatt
    17401 acgagaaggc gcgcgccttc ggcaagctgg tcgcgcgcta cagcgcgggc aagaaacccg
    17461 aggacatgct tttcatctgc accggcggcg gcccgggcat catgcaggcg gccaaccgcg
    17521 gggcctatga aggcgacggt atcagcgtgg gcctgtcgat cgcgctgccg atggaggaaa
    17581 gcgccaacgc atatgtcacg ccggccctgt cgttcaagtt ccactacttc gcgctgcgca
    17641 agatgcactt catgatgcgg gccaaggcgc tggtcgcgtt tcccggcggc ttcgggacac
    17701 tggacgaact gttcgaggtc atcaccctgg tgcagacccg caaggccagg caggtgccga
    17761 tcgtgctgtt cggcaccgac tactggaagc gcctggtcaa tttccaggtg ctgatcgacg
    17821 aaggcgtgat ctcggctgtg gacctcgacc tgttccagta cgcggacgag ccggatgcgg
    17881 cctgggacat catcaaggct ttctaccgac tctagtccac tgagcgcaag ctccgagaac
    17941 gctcaggccg acaggccgct tgaggccgtg gcggtcggcg tagcggccgg caggcagcgc
    18001 gaggaagacc tgggtcagcg cgaacagcgc cagcagcacg ccgacggcag cctcgctgtg
    18061 gccctggcgc agggccagca gcggcgccgc catgcgcatg ccggccatgc aggcatgcag
    18121 gaagatgtgg ccggcgatca ggcgcgccag cgccggattc agcccgccgg gcatccgagc
    18181 ctcctgtgga aacctccgct tctattcgcc tcggaagcgc cccgtcggct catgccggct
    18241 cttcttcgcc ttccacggga ggcagcagcg cctgggcctg cgcttcgggc agtgcttcca
    18301 ccaccttgag cgcgcggcgc atctggttgg tgcgggtctg cgcggtttcc agggtgtccg
    18361 aggccttgcg cacctgctcg cggatcttct cgacccagct gccatagcgt tcgaactcgg
    18421 tcttgacggc gccgagcacc ttccacactt cggacgcctg ctgctccagc gccagcgtcc
    18481 tgaagcccat gtgcaagctg ttgagcattg ccagcaacgt cgtgggaccg gccagcgtga
    18541 cgcggtagtc gcgctgcagg ctgtccatca ggccgggacg gcgcagcacc tcggcataca
    18601 ggctttcgat cggcaggaac aggatggcga aatcggtggt ctgcggcggg cacaggtagc
    18661 actcgctgat ggatttggct tcggtcctga tgcgcgcttc cagcgcacgc gcggcaatat
    18721 cggctgccgc cggatcggcg cggtcctgcg catccagcag tcgttcgtag tcgtcccgcg
    18781 ggaacttcgc atcgaccggc agccacaccg gtgttccgtc ggtgcgaccg ggaaaacgga
    18841 tcgcgaagtc gacgcgctgg ttgctgcgcg gcttggtctc cacctgtttg gcgtactgct
    18901 cgtgcgtcag cacctgctcg agcaaggcct cgagttgcac ctcgccgaac atcccccgcg
    18961 tcttgacgtt cgtcagcacg cgctgcaggt cgcccacgcc ctgggccagg gtctgcatct
    19021 cgcccagtcc cttgtgcacc tgctccagcc gatcggcgac ctgcttgaaa ctttccccca
    19081 gccgggtctc cagcgtggtc tgcagttttt cgtcgacggt catgcgcatc tcttcgagct
    19141 tggccgtatt ggtctgctgc agcatcgcca gttgcgcctc cagcgtgccg cggacttcgg
    19201 ccatgcgccg cgcgttcgac tcgctcaggt tctgcaactg cagcgacaac gtgtccgaaa
    19261 gcgtcttctg cagcagggtg agctgctggc cgaatgtgtc gatctgcgtg ttctgcgtgc
    19321 gcgtggcctc cgcgccctgc ttgaccaagg cgtcctggaa ggtggccagc gtgtgtgtga
    19381 gttcggtgcg cgcgccacgc gatgattcgc tgatttcacg gcgcagttcg cgctccatgc
    19441 gctcattggc ggcgagcagc tcggcccgcc ccgcttcatt gacgggcggc ttgcgcacga
    19501 acagcgcaac caccagcacc aggttggcga tggccagcgc gacgagggtc cagagttcca
    19561 cgtggcgctc tagttcagca cttcgggatt cagtggcgta aggggcttgc cgtggaccag
    19621 gaatccgatg aggttgtcgg ctgccaggtc ggccatcgct tttcgcgtcg gcacggtggc
    19681 gctggcgata tgcggcgtga gcacgacgtt gggcaccgtc agcagatccg ggtggacctt
    19741 gggctcgcct tcgtacacat cgagtccggc cgcggcgatg cgcttctcgc gcagggccgt
    19801 ggccaaagcc gcgtcatcga cgatgccgcc acgcgcgata ttgatcaggt tcgccgtcgg
    19861 cttcatcagg gccagctcgg ccgccccgat cgcatggtgc gacgcggggg agtacggcag
    19921 caccaggacg aggtgatcgg cggtccgcag caactcctcc ttgccgacat aacgcgcttt
    19981 gcactgcgcc tccagttcgg gtgcgaggcg ggaccggttg tggtagatca ccttcatgcc
    20041 gaagccatgg gccccgcgcc gcgcgatacc ctggccgatg cgtcccatgc ccaggatgcc
    20101 cagcatgctg ccatgcacgt ccgacccggc gaacatgtcg tagctccagc gttgccactt
    20161 gccggcccgc aggtaatgct cgctctcggt cacacgtcgc gccgccgcca tgagcagggc
    20221 gaatccgaag tcggccgtcg tttccgtgag aacgtccggc gtgttggtag ccaggacctt
    20281 gcgggccgtc atcgcgggca ggtcgaagtt gttgtagccc accgccatgt tcgcgcagat
    20341 cttcagctgc ggcgcggcgg ccagcacatc ggcgtcgata cgatcgcccc ccgtcgtgaa
    20401 cgcgccaagc ttgtcgtgaa gtttccgcgc g
//
LOCUS       DASWIY010000419        22291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2163411, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000419 DASWIY010000000
VERSION     DASWIY010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 22291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22291
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2163411"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..69)
                     /locus_tag="VGA59_14880"
     CDS             complement(<1..69)
                     /locus_tag="VGA59_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830471.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9721000.1"
                     /translation="MSDIEARVKKIIAEQLGVEESQV"
     gene            complement(214..960)
                     /gene="fabG"
                     /locus_tag="VGA59_14885"
     CDS             complement(214..960)
                     /gene="fabG"
                     /locus_tag="VGA59_14885"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX9721001.1"
                     /translation="MTPGFEGQVALVTGATRGIGAAIALELAQSGLKVIGTATTDEGA
                     SRIGSMLSAHAGCRGARLDVNDAAAAEALIDAVVKEHGGLHVLVNNAGITRDMLAMRL
                     KDEDWDAVLDTNLKAVFRMSRAVMRTMMKQRYGRIISITSVVGASGNPGQANYAAAKA
                     GVAGMTRALARELGSRAITVNCVAPGFIETDMTSALPEEQRKALLGQIPLGHLGQPAD
                     IAHAVAFLASPRAGYVTGQEIHVNGGMYMS"
     gene            complement(957..1907)
                     /gene="fabD"
                     /locus_tag="VGA59_14890"
     CDS             complement(957..1907)
                     /gene="fabD"
                     /locus_tag="VGA59_14890"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX9721002.1"
                     /translation="MKPLAFVFPGQGSQSVGMLDAWGGHPAVAQALQEASDALGEDLA
                     KLISQGPKEALALTTNTQPVMLVAGVAAYRAWRAEGGAEPSVLAGHSLGEYSALVASG
                     ALTLAEAVPLVRFRAQAMQDAVPVGTGAMAAILGMDAQGVIEGCAEAARSFGANSGEV
                     VEAVNFNDPAQTVIAGSKAAVEKACEVLKAKGAKRALPLPVSAPFHSSLMKPAADRLG
                     EKLAATPFAAPRIPVINNIAAKVETDPDRIRAALYEQAFGPVRWVECVQAIKARGITT
                     IVECGPGKVLAGLCRRIDAGLTGVALYDPPTLAEVKGMLA"
     gene            complement(1960..2937)
                     /locus_tag="VGA59_14895"
     CDS             complement(1960..2937)
                     /locus_tag="VGA59_14895"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX9721003.1"
                     /translation="MTRYSRISGTGSYLPPRRVTNADLAAELAARGVETSDQWIVERT
                     GIRARHFAAPEVTTSDLAVFAARQAIEAAGIGAKDIDLIILATSTPDMVFPSAACILQ
                     HKLGIAGCAAFDVQAVCSGFVYALTIADSMIKTGAANRALVIGAEVFSRILDFNDRTT
                     CVLFGDGAGAVVLEASDTPGILASDLHADGRHVGILCVPGTVSGGKVLGDPLLKMDGQ
                     AVFKLAVGVLEDAARAALAKAGRTAADIDWLIPHQANIRIMQGTARKLKLPMDKVVVT
                     VDEHGNTSAASIPLALDASVRSGKVKKGDTLMLEGVGGGFTWGAVLMDF"
     gene            complement(2938..3990)
                     /gene="plsX"
                     /locus_tag="VGA59_14900"
     CDS             complement(2938..3990)
                     /gene="plsX"
                     /locus_tag="VGA59_14900"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX9721004.1"
                     /translation="MTTLAVDCMGGDHGPHVTLAACRQFLEHHPDAQLLLVGVPDSLG
                     AFSHPRSRLVPASEVVAMDDPIEVALRRKKDSSMRVAIRQVKDGQAQAAVSAGNTGAL
                     MALARYLLKTLDGIDRPAIASPLPNAKGRSTTVLDLGANVDCTAQHLLQFAVMGSALV
                     SALTHVDAPSVGLLNIGEEAIKGSEVIKKAGELLRSAANAGDLNFYGNVEGDDIFKGT
                     TDIVVCDGFVGNVALKTSEGLATMVGGFLKEEFSRNVFTKLAAIAVYPMLFAFKKRVD
                     YRRYNGAALLGLRGLVFKSHGSADEFAFEQALNRAYDAARNNLLDRVQARIAHAKPLL
                     AAGEGNAQPLEVVATD"
     gene            complement(4109..4291)
                     /gene="rpmF"
                     /locus_tag="VGA59_14905"
     CDS             complement(4109..4291)
                     /gene="rpmF"
                     /locus_tag="VGA59_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX9721005.1"
                     /translation="MAVQQNKKSPSKRGMHRSHNALVVPGIAVEPTTGETHLRHHISP
                     TGFYRGRKVLKTKNEA"
     gene            complement(4420..4965)
                     /locus_tag="VGA59_14910"
     CDS             complement(4420..4965)
                     /locus_tag="VGA59_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX9721006.1"
                     /translation="MSKEFVARRLDVRRFAEEGAALSGRELVGEHPRLVAETRGRGAA
                     SPVTWSAIGELRNPRHVQPEIWLHLKAQAVLALTCQRCLGPVDAPLAVERSFRFVADE
                     DKAAAQDEQSEEDVLALSRTFDLVELVEDELLMEVPLAPRHESCPEPVKMEVADEDFD
                     AVGGRRENPFAILGKFKAGKS"
     gene            4991..5596
                     /locus_tag="VGA59_14915"
     CDS             4991..5596
                     /locus_tag="VGA59_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEX9721007.1"
                     /translation="MSEAPLPARHRPLVLGSTSPYRRELLSRLHLTFQTAAPEVDETE
                     RPGESPLELARRLALAKARSVAVQFPEAVVIGSDQVADLAGEALGKPGTHERAVAQLR
                     RMSGRSVIFHTAVAVVCAASGFEQLEVAPVRVAFRNLSDAEIESYLLAERPYDCAGSA
                     RSEGLGIALLDAIDSDDPTALVGLPLIRTCRMIGAAGVKVL"
     gene            5596..6360
                     /locus_tag="VGA59_14920"
     CDS             5596..6360
                     /locus_tag="VGA59_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9721008.1"
                     /translation="MSAPTGKLYLVPAPLDFACSVQAPLTDVMPAGTLACAAALTHWI
                     CENAKSARAYLKRIGELHPLALPLREQQIVELPRDVHKKGDHAGDFDATALLAPARQG
                     HDMGLVSEAGMPAIADPGSSVVRAAHGLGVEVVPLVGPVSLLLALAASGLNGQNFAFV
                     GYLPQDATQRAQRIRELEALAIGSGQAQLFIETPYRNPALWDALIKTLQPSTRLAVGS
                     GLTLPGAVTRSQPVKLWKQQGAPVRSDTPAVFALGR"
     gene            complement(6391..7440)
                     /locus_tag="VGA59_14925"
     CDS             complement(6391..7440)
                     /locus_tag="VGA59_14925"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX9721009.1"
                     /translation="MNESQPPESPGPENYPPAAGAQAAQASASPRTKAEGDRLGVGPE
                     WELATFEKLMFATLEEQRTARRWRSFIRLTWLAFFIFLLWALMYRGSPSADKSAPHTA
                     VIEIKGEIASDGEASAEGIVAGMRAAFEDTGAQAVVLLINSPGGSPVQAGIVNDEIKR
                     LRTKHKKPVYAVVEESCASAAYYIAAATDRIFVDKASIVGSIGVLMDGFGFTGLMDKL
                     GVERRLMTAGENKGFLDPFSPQTEKHRVFAQSMLDQVHLQFIEAVKAGRGKRLKDTPE
                     LFSGLFWTGQQAVELGMADQLGSLDFVAREVVKAEELVDYTRRENVAERLAKKFGAGI
                     GEGAMRALRTMPAFR"
     gene            complement(7444..7809)
                     /locus_tag="VGA59_14930"
     CDS             complement(7444..7809)
                     /locus_tag="VGA59_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX9721010.1"
                     /translation="MPAAIALCNSADLVDGGEAVPFDAIFCGQTCRAFAIRFDGRAHA
                     YLNRCAHVAMELDWQPNRFFDDTGRWLVCGTHGAVYRPDTGDCAGGPCRGALVKITLS
                     EQDGVVRWHTDYNLKPVEF"
     gene            complement(7812..8483)
                     /locus_tag="VGA59_14935"
     CDS             complement(7812..8483)
                     /locus_tag="VGA59_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX9721011.1"
                     /translation="MPPSPRPRQFDLIAFDWDGTLFDSTRIIVRCIQRAVVDVGGRRP
                     TDKAAAYVIGMGLAQALAHAAPDVPKEKYPELGARYRRHYIAQQNDLSLFDGVLPLLD
                     ELRARHHWLAVATGKSRRGLDEVLDTVQLRGMFDGSRTADETAGKPHPLMLQELMSEF
                     GTDPGRTLMIGDTTHDLQMALNAGCAAVGVSYGAHEPTEFDALKPLFIAHSVRDLHGW
                     LLENA"
     gene            complement(8452..9492)
                     /locus_tag="VGA59_14940"
     CDS             complement(8452..9492)
                     /locus_tag="VGA59_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX9721012.1"
                     /translation="MKQIIGGTASPASAKVKLVEVDEESAGQRLDNFLIRRLKGVPKT
                     HVYRIIRSGEVRLNKGRAAADTRVAAGDLVRLPPVRVSEKAGQKLEKPAPAREFPVLF
                     EDEHLIAVDKPAGVAVHGGSGVSFGVIEQLRQARPGAKFLELVHRLDRETSGILLVAK
                     KRSALTRLQDQFRERETGKTYLALVAGAWPANKKVIDMPLHKYLQADGERRVTVVAKD
                     DPDGMRAVTLVRLAQRISHPLPAGEGRGVGSGEGTFSLLEVTIKTGRTHQIRVHLASQ
                     GHPIAGDDKYGDFDLNKTLQKQGLKRMYLHAWRLQFNHPASGERVELLAGLPPELKKF
                     ADHAAVPAPPPV"
     gene            10072..13107
                     /locus_tag="VGA59_14945"
     CDS             10072..13107
                     /locus_tag="VGA59_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX9721013.1"
                     /translation="MKRMLINATQAEERRLAIVDGQKLLDYEIEIEGREQRKGNIYKA
                     VVTRVEPSLEACFVDYGEDRHGFLPFKEIARTYFAQGVPVSQARINDVIKEGQELLVQ
                     VEKEERGNKGAALTTFVSLAGRYVVLMPNNPRGGGVSRRIEGDDRAELKEAMDQLDYP
                     NGMSIIARTAGIGRSAPELQWDLNYLLKLWNAIDGAAKGGKGAFLIYQESSLVIRAIR
                     DYFNHDIGDILIDTDDIYEQAQQFMAHVMPEHAARVKRYRDDAPLFSRFQIEHQIESA
                     YAREVKLPSGGVVVIDHTEALVSIDVNSARAIKGGDIEETATRTNLEAADEVARQMRL
                     RDLGGLIVIDFIDMEESKNRREVENRLRDALRQDRARVQFGTISKFGLMEMSRQRLRP
                     ALSEGASIPCPRCGGHGHIRDTESSALQILRIIQEESMKDGTAAVHVQVPVEVASFLL
                     NEKRTEIAKIELKQRLNVIMVPNKTLETPNYRLERLKHDDPRLDNLQASYHMAEEIED
                     PTTVTRRTHERSNKQEPVIKGVLPDAPAPIAEVKPAPARPEPAAAKPAPRAPARGPAP
                     APAPAEHGFMAWIKGLFGVGPAPVAVPAPAVEAKKEEKREVQARGEGRGEGRRGGRGR
                     DGERRDGRRGEGRGRDGEPRREREARAEAGEGQPRTAEGGRGRDGEQRGAEAGRQRDG
                     EQRRGERQERAEGRGEREPREGRGEGRGERQGQRERRDGEPRGAVEGAGANLQAGAGA
                     TDQRPERPRGERGERGGERGEGRRERGEGRRGERRGERGPREPSVAAEGAEGAEVAAL
                     AGAATSVGLVPDMGDQQAPAADAVLESHTHQDARLADPQEGDERRDRRSRDRYGRDRR
                     ERGERQPREDGAVAPVAAADGESAPERDERPRYPTGFVSEEAQYAGVPAAATASPTPA
                     MAPAPAAPGLPKVQPFQLPVDELVQVAEGSGLQWVNSDAGKIAAVQAAIAAEPQPVRV
                     PRERPAAIVIDEGPLVLVETRRDLRELKLPFEQGSLPLQ"
     gene            complement(13130..14383)
                     /locus_tag="VGA59_14950"
     CDS             complement(13130..14383)
                     /locus_tag="VGA59_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEX9721014.1"
                     /translation="MRAALWLLALFGVAVAIALFAGNNQGTVTLFWPPHRLDLSLNLV
                     LLLLFAAFVFLHAALGALAALFDLPRQALRWRSQQKERAMHGAMLDALSHLLAGRFIR
                     ARKSAETALGQEKSLATGGQQPANAAQVRTLAHLLAAESAQALQDRTGREEHLKQALE
                     QASSREAQETHEGALMRAALWSLDDREPETAMAWLKGLPQGAARRTIALRMKLKAARL
                     TRHTAEALETARLLSKHRAFSAGAAESIVRGLAIDLLNDAHDVAQLQRAWNALEPDER
                     LMPELAIHAAQRLTTLGGDAHLARDWLLPVWERQSELGENLKVKLVRALEAGLDSFDG
                     AWLARIETAQKGDPREATLQYLAGMACMKRQLWGKAQQLMGQAAMGLKDATLHRNAWR
                     ALAQLAEERGDQESAAQAWKRAAGE"
     gene            complement(14396..15472)
                     /locus_tag="VGA59_14955"
     CDS             complement(14396..15472)
                     /locus_tag="VGA59_14955"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX9721015.1"
                     /translation="MSSEPSSPPPAVESQVPALDVVDAQARARPQGVLVARGLIYFFL
                     AVAGLSLTASVMLWQKLSTIQEQLARQSREAGGNAIEARALARQAQELARETAARQAL
                     SDTRLGEVALHRAQVEELIQSVSRSRDENLVVDIESGLRLAQQQAQLTGSAEPLLAEL
                     RSADKRLERAAQPRLSRVRAAIARDIDRVRAASVTDVPGLLVKLDELARLVDDLPLAN
                     AVAQAGDAGTARARAAPARLTWWEAMLADIREEARSLVRVSRVDQPDAVLLAPEQGFF
                     LRENLKFKLLNARLGLLSRQTDSARADVAAAIAAVRKYFDPAARKTQVAAGLLQQVQA
                     QLRAVELPRIDETLAVLATAAAGR"
     gene            complement(15469..16239)
                     /locus_tag="VGA59_14960"
     CDS             complement(15469..16239)
                     /locus_tag="VGA59_14960"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX9721016.1"
                     /translation="MRVLVTRPESEARHWVEELRRRGFDAQALPLIEIAPVADPAMLV
                     AAWRQLDRYRAVMFVSGNAVRHFFGHRPEGKPWPAPTRAWAPGGGTRDALLAARVTPE
                     LIDAPPPQAAQFDSETLWQQVAGQVGDGDRVLIVRGADAQGASSGRDWLAERVAAAGA
                     QVEVLPAYLRRPPVLTEGQLAQARCCGRTEGCVWLFSSSQAIEHLRTLLPGESWAQGR
                     AVATHPRIAQAARRAGFGVVCESRPSLAAVVAALESFR"
     gene            complement(16253..17200)
                     /gene="hemC"
                     /locus_tag="VGA59_14965"
     CDS             complement(16253..17200)
                     /gene="hemC"
                     /locus_tag="VGA59_14965"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX9721017.1"
                     /translation="MLASSLPRYYKQVTTLSKLVIATRESRLALWQAEHVKALLEQRG
                     HQVALLGMTTRGDQILDRSLSKVGGKGLFVKELEVALEEGHADLAVHSLKDVPMDLPQ
                     GFALGCVMEREDPRDAFVSGRVATFAELPQGAIVGTSSLRRVVLLRSLRPDLKIEPLR
                     GNLDTRLRKLDEGQYDAIVLAAAGLKRLGLEARIRHVFEPEHMLPSAGQGALGIEVRA
                     HRTDLLELLAPLSHQATWLAVAAERAVSRAMGGSCSMPLAAFATFSGEYLQLRAAWGE
                     PEGSTALVRAQSAVAAADLAQAAELGAAVAEQLRAAGAH"
     gene            17364..20255
                     /locus_tag="VGA59_14970"
     CDS             17364..20255
                     /locus_tag="VGA59_14970"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA];
                     GO_process: GO:0048366 - leaf development [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX9721018.1"
                     /translation="MPPSPQSPSRRAKDNERPLLEDIRLLGRILGDVIRDQEGVEAFE
                     LVERIRKLSVSFRRDADDEADKALKSLLKSLSSEQTMSVIRAFTYFSHLANLAEDRHH
                     IRRRAIHERAGDTQEGSIEVALARLRWAGIGAKTVSQTLAHSYVSPVLTAHPTEVQRK
                     SILDAERGIANLLTQRDEIKANAAPKDALAPRELAANEAGIRARVLQLWQTRLLRFTK
                     LAVADEIENALSYYESTFLREIPKIYADLERELGHHPVAAFLRMGQWIGGDRDGNPNV
                     NAGTLEYALRRQCEVALRHYLTEVHYLGGELSLSSTLVEVTPAMRALADSSPDNNQHR
                     LDEPYRRALAGMYSRLAATLKEFTGGDAARHALPPKNPYLRAEDFLADLRTIEDSLLA
                     QHAGALVQQRLHPLIRAVEVFGFHLATVDLRQSSDQHEAVVAELLAVARIEPHYARLA
                     EEARRALLMGLLADARPLRVIGGRYSEHTRAELAIFETARLMRERFGPQAIRHYIISH
                     TETASDLLEVLLLQKEAGLLTGTIDDQAVADLIVVPLFETIEDLRNAPGIMREFYGLP
                     GIAALVRRSGAEQDIMLGYSDSNKDGGIFTSNWELYRAEIALVELFDQLATRGAGPPQ
                     DAGASSRGSAAHAAASGGAHGPHEIQLRMFHGRGGTVGRGGGPSYQAILAQPPGTVRG
                     QIRLTEQGEVIGSKYANPEIGRRNLETLVAATLEATLLQPTKTAPKAFLVAAETLSRT
                     SMAAYRALVYETPGFADYFSGSTPIREIAELNIGSRPASRKPSLRIEDLRAIPWSFSW
                     GQCRLTLPGWYGFGTAVHQFLHENPAHERRAALALLQKMYRQWPFFGALLSNMDMVLA
                     KSDLALASRYAQLVADARLRKRIFTTIEAEWHRTVEAMQAITGEKQRLASNPALQRSI
                     RHRFPYIDPLHHLQVELVRRYREGKIDDLARRGIHISINGIAAALRNTG"
     gene            complement(20283..21449)
                     /locus_tag="VGA59_14975"
     CDS             complement(20283..21449)
                     /locus_tag="VGA59_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9721019.1"
                     /translation="MEEKPDAPAAAPLAADPDDPSHALLHVPVNVRSAALVLLTVLAV
                     LVMLRWASAFFIPLMLGFMFSYALSPIVRALERWRIPRGISAAVLILGILGGTGASVY
                     AFSDDANQLIESLPAAAKKLRDSMRGRTGKTDTPLETVQKAAAQLEQAAQDAGPAAPA
                     GRGVQRVVIEKPRFDIREHLWSGTVGLASLIGQVVVVTFLTFFLLLSGDTFRRKLVKI
                     TGPTLANKRITVQALDEITAQIQRYLLVQLLASIVVGVTTGLAFWALGLKHAAVWGFA
                     AGLLNLIPYIGSVVVTGAAGLVAFLQFGGINMALTVAGISLLINTIEGNLLVPWLTSK
                     ASRMNPVAVFIGVLGWGWLWGVWGLLLGIPILMVVKAICDRVDDLKPVGELLGT"
     gene            complement(21480..22220)
                     /locus_tag="VGA59_14980"
     CDS             complement(21480..22220)
                     /locus_tag="VGA59_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX9721020.1"
                     /translation="MSLQVLLVDDEALARSRLRTLLGDCGAPTAAVAGEASNSVQAME
                     LLQRLTFDVVLLDIHMPGADGLALAQALRSLPRPPAVVFITAHAEHAVAAFELEAVDY
                     LTKPVRLERLQAALQKVERLAQAKGGAQHAGASEILLIQDRGRTERVPLAEVLYFKAE
                     LKYITVRTAAKSYILDTSLSELEERHAAQFLRVHRNALVARHAIRALEKHYDPEEGEG
                     WAVRLNGIDEVLAVSRRQLSVVREALAA"
BASE COUNT         3635 a         7661 c         7426 g         3569 t
ORIGIN      
        1 gacctgggac tcttccacgc cgagttgctc ggcgatgatt tttttgacac gtgcttcgat
       61 atcgctcatg gttccctcag agggttgtga atgaatccgc gattttagct ccggcataac
      121 aagcccttcg ggcagttgtt agcctgcgct gaaagctctt cgagctttta gcctgccctc
      181 agccgggcgg ataaaccggc ttgaacccgt ttttcaggac atgtacatgc cgccgttgac
      241 gtggatttcc tgtcccgtca catacccggc cctgggcgat gcgaggaatg ccaccgcatg
      301 ggcgatgtcc gcgggctggc ccaggtgccc caacggaatc tgtcccagca atgccttgcg
      361 ctgctcttcc ggcaaggccg aggtcatgtc ggtttcgatg aagccgggcg cgacgcaatt
      421 gaccgtgatc gcccggctcc cgagttcgcg cgccaatgcg cgcgtcatcc cggcgacacc
      481 ggccttggcg gccgcgtagt tcgcctgccc cggattgccc gaagcgccga cgaccgaggt
      541 gatgctgatg atgcggccat agcgctgctt catcatcgtg cgcatcacgg cccggctcat
      601 gcggaaaacg gccttcaggt tggtgtcgag caccgcgtcc cagtcctcgt ccttcagccg
      661 cattgccagc atgtcgcgcg tgatgcccgc gttgttgacg agcacatgca agccgccgtg
      721 ctccttcacg acggcatcga tcagcgcctc ggcggcggcc gcatcgttga catccagcct
      781 ggcgcccctg catccggcat gggcggacaa catgctgccg atgcgtgacg caccttcgtc
      841 cgtcgtggcg gtgccgatga ctttcaggcc gctttgcgcc aactcgagcg cgatagcggc
      901 accgatgcca cgggtggcgc cggtgaccag ggcgacctgc ccttcgaacc cgggcgtcat
      961 gcgagcatcc ccttcacttc ggccagggtg ggcgggtcat acagggccac gcccgtgaga
     1021 ccggcatcga tgcgcctgca cagtccggcc agcaccttgc ccggcccgca ttcgacgatg
     1081 gtcgtgatgc cgcgggcctt gattgcctgc acgcactcga cccaacgcac cggtccgaac
     1141 gcctgctcat agagcgcggc gcgaatgcgg tcggggtcgg tctcgacctt cgctgctata
     1201 ttgttgatca ccgggatccg tggcgccgcg aacggcgttg ccgcgagctt ctccccgagc
     1261 ctgtccgcag ccggcttcat caggctggaa tggaacggcg ccgacaccgg caggggcaag
     1321 gcgcgcttgg cgcctttggc cttgagcacc tcgcaggctt tctcgaccgc cgccttgctg
     1381 ccggcgatca cggtttgcgc cggatcgttg aagttcacgg cctccacgac ctcgccggag
     1441 ttggcgccga agctgcgcgc cgcctcggcg cagccttcga tcacgccttg cgcgtccatc
     1501 ccgaggatcg ccgccatcgc gccggtgcct acaggcaccg cgtcctgcat cgcttgcgcg
     1561 cgaaagcgaa ccagcggcac ggcctcggcc agcgtcagcg cgcccgaagc caccagggcc
     1621 gaatactccc cgagcgaatg acccgcgagc acggaaggct ccgcgccgcc ttcggcgcgc
     1681 caggctcgat aggccgcgac acccgcaacc agcatcacgg gctgggtgtt ggtggtcagc
     1741 gccagcgctt ccttgggccc ctggctgatc aacttcgcca ggtcctcgcc cagcgcgtcg
     1801 gaagcttcct ggagcgcctg ggccacggcc gggtggccgc cccaggcgtc gagcatgccg
     1861 acggactgcg agccctggcc cggaaagacg aatgcaagcg gtttcatgcg cgttaccaac
     1921 tatgaaatga ggcggggcgg gagcgcgagc gggacgcgcc tagaaatcca tcaggacggc
     1981 gccccacgtg aagccgcctc ccacgccctc gagcatgagg gtgtcgccct ttttcacttt
     2041 gccgctgcgc accgaagcgt ccagggcaag ggggattgaa gcggcggagg tgttgccgtg
     2101 ctcgtccacc gtcacgacga ctttgtccat cggcagcttc agcttcctgg cggtgccctg
     2161 catgatgcgg atattggcct ggtgggggat cagccaatcg atgtcggctg ccgtcctgcc
     2221 ggccttggcc aacgcggcgc gggcggcgtc ttccagcacg cccaccgcca gtttgaacac
     2281 cgcctggccg tccatcttga gcagagggtc acccagcacc ttgccgccgg ataccgtccc
     2341 cgggacacac aggatgccca cgtgcctgcc gtcggcgtgc agatcgctcg ccaggatgcc
     2401 cggcgtatcg gacgcttcga gcaccacggc ccccgcgccg tcaccgaaca gcacgcaggt
     2461 cgtgcgatcg ttgaagtcga ggatgcgcga gaacacttcg gcgccgatca ccagggccct
     2521 gttcgcggca cccgtcttga tcatggagtc ggcgatggtc agcgcataga cgaagccgct
     2581 gcaaacggcc tgcacgtcga acgcggcgca accggcgatg cccagcttgt gttgcaggat
     2641 gcaggccgcg gaagggaaga ccatgtccgg cgtggacgtg gcgaggatga tcaggtcgat
     2701 gtctttcgcc ccgatgccgg ccgcctcgat cgcctgccgc gccgcgaaca cggccaggtc
     2761 gctggtcgtc acctcgggtg ccgcaaagtg gcgcgcgcgg atgccggtgc gctccacgat
     2821 ccactgatcg gaggtttcca cgccccgggc cgcgagttcc gcggccagat cggcgttggt
     2881 cacgcggcgc ggcggcagat agctgccggt gccgctgata cgggaatatc ttgtcattta
     2941 gtcggtcgcg acgacttcca gcggctgggc attgccttcg ccggccgcca gcagcggctt
     3001 ggcatgggcg atgcgcgcct ggactcggtc gagcaggttg tttcgggccg catcatacgc
     3061 ccggttcaga gcttgctcga aggcgaactc gtctgccgaa ccatgactct tgaacaccag
     3121 gccgcgcagg cccagcagcg ccgcaccgtt gtatcgccgg tagtctacgc gctttttgaa
     3181 tgcaaatagc atcgggtaca cggcaattgc cgccagtttg gtaaagacgt ttcgggaaaa
     3241 ctcctccttg aggaagccgc cgaccatcgt ggccagccct tcgctggtct tcaaggcgac
     3301 attgcccacg aagccgtcgc acacgacgat atcggtggtt cccttgaata tgtcgtcccc
     3361 ttcgacgttg ccatagaagt tcagatcgcc ggcgttagcc gcagatcgca gcaactcgcc
     3421 ggctttcttg atgacttcgc tgcctttgat ggcttcttcg ccaatgttca acagccccac
     3481 cgatggcgca tccacatgcg tcagggccga aacgagggcc gagcccatga cggcgaactg
     3541 aagcaggtgc tgcgcggtgc aatccacgtt cgcgccgaga tccaggactg tggtcgaacg
     3601 gcctttcgcg ttgggcaggg gagacgcgat ggccggacgg tcgatgccgt ccagcgtctt
     3661 gagcaggtaa cgcgcgagcg ccatcagcgc accggtattg ccggccgaca ccgcggcctg
     3721 ggcctggccg tccttgacct ggcggatggc gacgcgcatc gaggagtctt tcttcctgcg
     3781 caacgccact tcgatcgggt cgtccatggc caccacttcc gatgccggca ccaggcggga
     3841 gcgcggatgg ctgaaagcgc caaggctgtc gggcacgccg accaggagca gctgcgcatc
     3901 gggatggtgc tcaaggaact gccggcaggc cgcgagcgtg acgtggggac catggtcgcc
     3961 ccccatgcag tcgacagcca gagtagtcat gggctcaaat gtagagtctt ttggcggcgg
     4021 gtcccgcgcc acgcgtttgc ctggtgtcgg accaaaacaa aggcccgcgc tgcgcacgcg
     4081 cagccgggcc tcgaggatgc aatgccggtc aggcttcgtt cttggtcttg agcaccttgc
     4141 ggccacggta gaagccggtc gggctgatgt ggtgacgcag gtgggtttcg ccggtggtgg
     4201 gttccacggc gatgcccggt acgacgagcg cgttgtgcga gcgatgcatg ccgcgcttgg
     4261 agggcgattt cttgttctgc tggacggcca tgaggggctc ctggattcaa aatggccccg
     4321 cgcggggcgc aaggcggttg agttgggtgg gtgggcaccc acgaagcggc aaggcctggg
     4381 ggcgcgaagc ccatgattat agcccaactc gttggtttgc taggacttgc cggccttgaa
     4441 cttgcccagg atcgcgaagg ggttctcgcg ccggccaccg acggcatcga agtcttcgtc
     4501 ggccacttcc attttcaccg gctccgggca gctttcgtgg cgcggcgcca gcggcacctc
     4561 catcagcagt tcatcctcga ccagttcgac cagatcgaaa gttcggctca atgccagcac
     4621 gtcctcctcg gattgctcgt cctgggccgc ggccttgtcc tcgtcggcaa cgaagcggaa
     4681 ggagcgttcc accgcgagcg gtgcatccac cggccccagg caacgctggc aggtcagggc
     4741 cagcacggcc tgcgccttca ggtgcagcca gatctccggc tggacatggc gtggattgcg
     4801 cagttcgccg atggcggacc aggtgacggg ggacgcggcg ccccgtcccc gtgtctcggc
     4861 caccagacgc gggtgctccc ccaccaactc ccggccgctc agcgcagcgc cttcctcggc
     4921 gaaacgcctc acatccaggc ggcgggccac gaactccttg ctcattgcgc cagtgtaaga
     4981 gaatcgggcc atgtccgagg ctccccttcc cgctcgacac cggccgctgg tgctgggctc
     5041 gacttccccc tatcgccgcg agcttctgtc gcgcttgcac ctgacattcc agacggctgc
     5101 gcccgaggtg gacgagaccg agcgccccgg cgagtcgccg ctcgaactgg cgcggcgcct
     5161 ggccttggcc aaggcccgct cggtggctgt gcaatttccc gaggccgtgg tgatcgggtc
     5221 ggaccaggtc gcggatctgg ccggcgaagc cctgggcaaa ccgggcacgc atgaacgggc
     5281 cgtcgcccag ctgcggcgga tgagcggccg cagcgtcata ttccacacgg ccgtcgccgt
     5341 tgtatgcgct gccagcggat tcgagcagct ggaagtggcg ccggtcaggg tcgccttccg
     5401 caacctcagc gatgcggaaa tcgaatccta tctgctggcg gaacggccct acgactgtgc
     5461 cggcagcgcc cgcagcgaag gcctgggcat tgcgctgctg gatgcgatcg acagcgatga
     5521 cccgacggcg ctggtgggcc tgccgctgat acgcacctgc cgcatgatcg gggcagcggg
     5581 cgtgaaggtg ctgtgatgtc ggcgccgacc ggcaagctgt acctggttcc ggcgcccctc
     5641 gatttcgcct gtagcgtcca ggcgccgctc acggacgtga tgccggccgg caccctggcg
     5701 tgcgccgcgg cgttgacgca ctggatttgc gagaacgcca agtccgcgcg cgcctacctc
     5761 aagcgtattg gcgagctgca cccgctggcc ctgccgctgc gggagcagca gatcgtcgag
     5821 ctgccccggg acgtgcacaa gaaaggcgac catgcaggcg acttcgacgc caccgccctg
     5881 ctcgcgccgg cccggcaagg ccacgacatg gggctggtca gcgaggcagg catgccggcg
     5941 atcgcggacc cgggctcgtc ggtggttcgc gccgcccatg gcctgggcgt ggaagtcgtg
     6001 cccctggtcg ggccggtctc gctgctgctg gcactggccg cgagcggcct caacggccag
     6061 aatttcgctt tcgtcggcta cttgccgcag gatgcgacgc agcgggccca gcgcatccgg
     6121 gagctcgaag cgctggccat cgggagtgga caggcgcagc tcttcatcga aacgccctat
     6181 cgcaaccccg cactctggga tgctctgatc aagaccttgc agccctcgac gcgcctggcc
     6241 gtgggcagcg gcctcacgct gcccggcgcc gtgacccgca gccagccggt caagctctgg
     6301 aagcagcagg gtgcgcccgt cagaagcgac acccccgccg tcttcgcgct cggccgctag
     6361 cgcccgtgcg actggttttg cgggttcctg tcagcgaaat gcaggcatcg tcctgagcgc
     6421 gcgcattgcg ccttccccga tcccggcgcc gaacttcttg gccaggcgct cggccacgtt
     6481 ttcgcggcgg gtgtagtcga ccagttcctc ggccttcacc acttcgcgcg cgacgaagtc
     6541 gaggctgccc agctggtcgg ccatgcccag ttcgaccgcc tgctggccgg tccagaacag
     6601 gccgctgaac aactccggcg tgtccttcaa tcgcttgccc cggcccgcct tcacggcttc
     6661 gatgaactgc aggtgcacct gatcgagcat cgactgcgcg aagacccgat gcttttcggt
     6721 ctgggggctg aacggatcga ggaagccctt gttttcgccg gccgtcatca ggcgtcgctc
     6781 gacgccgagc ttgtccatga ggccggtgaa tccgaagccg tccatcaata cgccaatgct
     6841 gccgacgatg ctggccttgt cgacgaagat ccggtcggtt gccgccgcga tgtaataggc
     6901 ggcggacgcg caggactcct ccaccacggc atagaccggt ttcttgtgct tggtcctcaa
     6961 gcgcttgatt tcgtcgttga cgattccggc ctgcaccgga ctgccgccgg gcgagttgat
     7021 caacaacacc acggcctggg ccccggtatc ctcgaaagcc gcgcgcatcc ccgccacgat
     7081 cccctcggcg ctggcctcgc cgtcggaggc gatttcgccc ttgatttcga tcaccgccgt
     7141 atgcggcgcg gacttgtcgg cgctgggcga gccgcgatac atcaatgccc agagcaggaa
     7201 gatgaagaat gccagccacg tcagccggat gaagctgcgc cagcggcggg ccgtgcgctg
     7261 ttcctccagg gtcgcgaaca tcagcttttc gaaagtggcc agttcccact ccggcccgac
     7321 cccaagccga tcgccttccg ctttggtcct ggggctcgca ctggcttggg cggcctgggc
     7381 gccggccgcg ggcgggtaat tttccgggcc cggcgattcg ggcggctgcg attcattcat
     7441 gttctagaac tctacaggtt tgaggttgta atcagtatgc cagcggacca cgccgtcctg
     7501 ttcgctgagg gtgatcttca cgagagcgcc gcgacacggg ccgccggcgc agtctccggt
     7561 gtcgggccgg tagaccgcgc cgtgggtgcc gcacaccagc cagcggccgg tgtcgtcgaa
     7621 gaagcgattg ggctgccagt cgagctccat cgccacatgg gcgcagcggt tgaggtaggc
     7681 atgcgctcgc ccgtcgaatc ggatcgcgaa cgcgcggcag gtctggccgc aaaagatcgc
     7741 atcgaagggc accgcctcgc cgccgtccac caggtcggcc gaattgcaca gcgcgatcgc
     7801 agcgggcatg ttcaagcgtt ttccaacagc cagccatgca agtcgcgcac cgagtgggcg
     7861 atgaatagcg gcttcagggc gtcgaattcc gtgggctcgt gagcaccgta gctcacgccc
     7921 acggcagcgc agccggcgtt gagcgccatc tgcaggtcat gcgtggtgtc gccgatcatc
     7981 agcgtgcgcc cggggtcggt cccgaattcg ctcatcaatt cctggagcat gaggggatgg
     8041 ggcttgcccg cggtttcgtc ggccgtgcgg gagccgtcga acatgcccct gagctgcacc
     8101 gtatccagga cttcgtccag cccacggcgc gactttcccg tcgccaccgc cagccagtga
     8161 tgccgggccc tgagctcgtc cagcagaggc agcacgccgt cgaacaggct caggtcgttc
     8221 tgctgcgcga tgtaatggcg ccggtagcgc gcgccgagtt cgggatattt ctccttcggc
     8281 acatccggcg cggcatgggc cagtgcctgc gcgaggccca tgccgatcac ataggccgcg
     8341 gccttgtcgg tcggcctcct gccgccgacg tccaccacgg cacgctggat gcagcgcacg
     8401 atgatccggg tggagtcgaa aagggttcca tcccagtcga aggcgatcag atcaaactgg
     8461 cgggggcgcg gggacggcgg catggtcggc aaacttcttc agttccgggg gtagccccgc
     8521 gagcagctcg acgcgctcgc cgctggcagg atgattgaac tgtaaccgcc acgcgtgcag
     8581 atacatgcgc ttgagccctt gcttttgcag cgtcttgttg aggtcgaaat cgccgtactt
     8641 gtcgtcgccc gcgatgggat ggccctgcga cgccagatga acgcggatct ggtgggtccg
     8701 tccggtcttg atggtgactt cgaggaggga gaaggttcct tcgcccgaac ccacaccccg
     8761 gccctctccc gcagggagag ggtgagaaat gcgctgcgcg agcctcacga gggtcacggc
     8821 cctcatgccg tccgggtcgt ctttggccac caccgtcacc cggcgctcgc cgtcggcctg
     8881 caggtacttg tgcagcggca tgtcgatgac cttcttgttg gcgggccagg cgccggccac
     8941 tagcgccagg taggtctttc ccgtctcgcg ctcccggaac tggtcctgca ggcgggtcag
     9001 ggcagagcgc ttcttggcca ctagcaggat gcccgatgtt tcccggtcca gccggtgcac
     9061 cagttccagg aacttcgctc cggggcgcgc ctggcgcagc tgttcgatca cgccgaagct
     9121 cacgccgctg cctccgtgca cggccacgcc ggccggcttg tcgacggcga tcaggtgctc
     9181 atcctcgaaa agaacgggaa attcccgcgc cggcgccggt ttttccagct tttgccccgc
     9241 cttttcggaa accctcaccg gcggcagccg taccaggtcg cccgccgcca cccgcgtgtc
     9301 ggctgccgcc cggcccttgt tcaagcgcac ttcgccgctg cggatgatgc ggtaaacatg
     9361 ggttttcggc acgcccttga gccggcggat gagaaagtta tcgagccgct ggccggcgga
     9421 ttcttcgtcc acctcgacta atttgacctt cgcgcttgca ggggacgccg tgccccctat
     9481 aatctgtttc actagcgtta cgctgtaagt ggttgattcg ccttggagtt tagtccacgc
     9541 gctgccaccg gccacgctgg aaggtcgatg ccaccgagac ccccgcgcgc aggttgccag
     9601 ccagatgctg gcaatggccc gccaaggtcg cggtcaagcc ggcactttga aacactgata
     9661 cggaatacca gtccgcaagc ccgatgcttt gcggacggac gaagcgagaa ctcctgtgtt
     9721 gatggcgatc ctcatggcct gctggcggtg gctcacagcc cccgttgaag gcttggcctc
     9781 gccgcccgac cgcgccgagc gggcggggta cgctgcacgc cctgcagcgc ctcagtgccg
     9841 ccgcttgggc ggcttgttct tcgcccccat ccaaacgccc ttgccgcgcc tgctgagtta
     9901 gcactccgta gccgtgtcgc ttgccggccc attccgttcg tttcgaagcg tctgcctgag
     9961 catcgttgac ccgttcgggt ctcgttcgaa gtcgtatgac ttcgcgggac ggaactgcga
    10021 atcgctctgt cgatccgctc cgtccccgac tgactagaag aaggtgacgc catgaagcgc
    10081 atgctgatca atgccacgca ggcggaagaa cgccggctgg cgatcgtcga tggacaaaaa
    10141 ctcctcgact atgaaatcga aatcgaaggg cgcgagcagc gcaagggcaa tatctacaag
    10201 gccgtcgtga cgcgcgtcga gccttcgctc gaggcctgct tcgtcgatta cggcgaagac
    10261 cgccacggct tcctgccgtt caaggagatc gccaggacct atttcgcgca gggagtgccc
    10321 gtctcccagg cccgcatcaa cgacgtgatc aaggaaggcc aggagctgct ggtgcaggtc
    10381 gagaaggaag agcgcggcaa caagggcgcg gccctgacca ctttcgtctc gctcgccggt
    10441 cgctatgtgg tgctgatgcc gaacaatccg cgaggcggcg gtgtcagccg ccgcatagag
    10501 ggcgatgacc gcgccgaact caaggaggcg atggaccagc tggactaccc gaacggcatg
    10561 tccatcatcg cgcgtaccgc cggcatcggc cgcagcgcgc cggaactgca atgggacctg
    10621 aactacctgc tcaagctgtg gaacgccatc gacggcgcgg ccaagggcgg caagggcgcg
    10681 ttcctgatct accaggagtc gtcgctggtc atccgtgcca tccgcgacta cttcaaccac
    10741 gacatcggcg acatcctgat cgataccgac gacatctacg agcaggccca gcagttcatg
    10801 gcccacgtca tgcccgagca tgccgcccgc gtgaagcgct accgcgacga cgcgccgctg
    10861 ttttcgcgct tccagatcga gcaccagatc gaatcggcct atgcgcgaga agtgaagctg
    10921 ccctccggcg gcgtggtcgt gatcgaccac accgaggcgc tggtgtcgat cgacgtcaat
    10981 tcggcgcgcg cgatcaaggg cggcgacatc gaggaaaccg ccacccgcac caacctggaa
    11041 gccgccgacg aggtggcacg ccagatgcgc ctgcgcgacc tgggcgggct gatcgtgatc
    11101 gacttcatcg acatggagga gtcgaagaat cgccgcgagg tcgagaaccg cctgcgcgac
    11161 gcgctgcggc aggaccgtgc ccgcgtgcag ttcggcacga tcagcaaatt cggtctgatg
    11221 gaaatgagcc gccagcggct gcgccccgcc ctgtccgaag gcgcatccat cccctgcccg
    11281 cgctgcgggg gccacgggca tatccgcgac acggaaagct ccgcgctgca gatcctgcgg
    11341 atcatccagg aagaatccat gaaggacggc accgccgccg tgcacgtgca agtgccggtg
    11401 gaagtggcgt ccttcctgct gaacgaaaag cgcaccgaga tcgccaagat cgagctcaag
    11461 cagcgcctga acgtgatcat ggtgcccaac aagacgctgg aaacgcccaa ctaccggctc
    11521 gagcgcctca agcacgacga tccacgtctg gacaacctgc aggccagcta ccacatggcc
    11581 gaggaaatcg aggatccgac caccgtcact cgccgcaccc atgagcgcag caacaagcag
    11641 gagccggtga tcaagggcgt gctgccggac gcaccggcgc cgatcgccga ggtgaagccg
    11701 gcgccggccc gacccgagcc ggccgcggcg aagcccgcgc cgcgcgcacc cgcccggggc
    11761 ccggccccgg cacccgcgcc ggccgaacat ggcttcatgg cgtggatcaa gggcctgttc
    11821 ggcgttggcc cggcaccggt ggccgtgccg gcaccggcgg tcgaggcgaa gaaggaagaa
    11881 aagcgcgagg ttcaggcgcg cggcgaaggc cggggcgagg ggcgccgcgg cgggcgcggt
    11941 cgtgacggcg agcgccgtga cggccggcgt ggtgaaggac gcgggcgcga tggcgaaccc
    12001 aggcgtgagc gcgaagccag ggccgaggcc ggcgagggcc agccgcgtac cgccgagggt
    12061 gggcgtggcc gcgacggcga gcaacgcggt gccgaggccg gccgccaacg cgacggcgag
    12121 cagcgccgcg gcgaacggca ggagcgcgca gagggccgag gcgagcgcga gcctcgcgag
    12181 ggccgcggtg agggccgagg cgaacgccag ggacagcggg agcgccggga cggcgagccg
    12241 cgcggcgctg tcgaaggcgc gggcgcgaac ctgcaagccg gcgccggcgc gaccgatcaa
    12301 cgcccggagc ggccgcgtgg cgaacgcggt gagcgcggtg gcgagcgcgg cgagggccgg
    12361 cgcgagcgcg gcgagggtcg ccgtggcgaa cgccgcggcg aacgcggccc gcgcgagccg
    12421 tccgtagccg ccgagggcgc cgagggcgcc gaagtggccg ccctggccgg cgccgccacc
    12481 tcggtgggcc tggtacccga tatgggcgat cagcaggcac ccgccgccga tgccgtgctc
    12541 gagtcgcaca cccaccagga tgcgcgtctg gccgacccac aggaaggcga cgagcgccgc
    12601 gaccgccgct cgcgcgaccg ctatggccgt gatcgccgcg agcgcggcga acgccagccg
    12661 cgtgaggacg gcgccgtcgc gcccgttgcc gcggccgatg gcgagtccgc gcctgagcgc
    12721 gatgagcgcc cgcgttatcc gaccggcttc gtcagcgagg aagcgcaata cgccggcgta
    12781 cccgcggcgg ctactgcgtc gcccacgccg gccatggcgc cggcaccggc tgcgccgggc
    12841 ttgcccaagg tccagccctt ccagctgccg gtcgacgagc tggtccaggt ggcggaaggt
    12901 tcgggactgc aatgggtcaa ttcggatgcc gggaagatcg ccgccgtgca ggcggccatc
    12961 gccgccgagc cgcagcccgt gcgcgtgccg cgcgagcgtc cggcggccat tgtcatcgat
    13021 gaggggccgc tggttttggt ggaaaccagg cgcgacctgc gcgagctgaa gctgcccttc
    13081 gaacagggca gcctgccgct gcaataagct gcgcgcgggg gccgaaacac tattcgccgg
    13141 ccgcgcgttt ccaagcctgg gccgcgcttt cctgatcgcc gcgttcctcg gccagctgcg
    13201 ccagtgcgcg ccaggcgttg cggtgcagcg tcgcgtcctt cagtcccatg gcggcctggc
    13261 ccatcagctg ctgcgccttg ccccagagct gacgcttcat gcaggccatg ccggccaggt
    13321 actggagcgt ggcttcacgc gggtcgcctt tctgcgcggt ttcgatccgc gccagccagg
    13381 caccgtcgaa gctgtccagg cccgcttcca gggcgcggac cagtttcact ttcaggttct
    13441 cgcccagctc cgactggcgt tcccacaccg gtagcagcca gtcgcgtgcc aggtgggcgt
    13501 cgccgccaag ggtggtgagg cgttgcgcgg cgtgaatggc cagctcgggc atcaggcgct
    13561 cgtcgggctc cagcgcgttc caggcgcgct gcagttgcgc gacgtcgtga gcgtcgttga
    13621 gcaggtcgat agccagcccg cgcacgatgc tctcggcggc gcccgccgaa aaagcccggt
    13681 gcttggacaa caggcgggcc gtttccagcg cctcggccgt atgccgcgtg aggcgtgccg
    13741 ctttcagctt catgcgcagt gcgatcgtgc gacgcgctgc gccctggggc aggcctttga
    13801 gccacgccat cgccgtttca ggctcgcggt cgtccagcga ccagagcgcc gcgcgcatca
    13861 acgcgccctc gtgcgtttcc tgcgcttcgc gcgatgatgc ttgttcgagg gcctgcttca
    13921 ggtgttcctc gcggccggtg cggtcctgca gtgcctgcgc gctttcggct gccagcaggt
    13981 gagccagggt acgcacctgc gccgcgttgg ccggctgctg cccgcccgtc gccagtgatt
    14041 tctcctgccc gagggcggtc tcggccgact tgcgggcgcg aatgaagcgg ccggccagca
    14101 ggtgcgacag cgcatcgagc atcgccccat gcatggcccg ctccttctgc tgcgaacgcc
    14161 agcgcaatgc ctggcgcggc aggtcgaaca gcgccgccag ggcccccagc gcggcatgca
    14221 ggaacacgaa cgccgcgaac agcagcagca gcacgaggtt gagggaaagg tcgagccggt
    14281 ggggcggcca gaacagggtg acagtgccct ggttgttgcc cgcgaacagc gcgatggcga
    14341 cggccacgcc gaacagggcc aggagccaga gcgcggcgcg catggctggc ctgccttacc
    14401 ttcccgcggc ggccgtcgcc agcacggcca gcgtttcgtc gatgcgcggc aattcgacgg
    14461 cgcgcagctg ggcctgcacc tgctggagca agcccgcggc cacctgggtc ttgcgggccg
    14521 cggggtcgaa gtacttgcgc acggcggcta tcgcggccgc gacatccgcg cgcgccgaat
    14581 ccgtctgccg ggacaacagg cccagcctcg cattgagcag cttgaacttg aggttttcgc
    14641 gcaggaagaa tccttgctcg ggggccagca gcaccgcgtc aggctggtcg acgcggctga
    14701 ctcgcacgag gctgcgtgcc tcttcgcgta tatccgccag catggcctcc caccaggtaa
    14761 gccgggccgg tgcggcccgc gcgcgcgctg tcccggcatc gcccgcctgc gccacggcat
    14821 tggcgagcgg cagatcgtcg accagccgcg ccagctcgtc gagcttgacg agcaggccgg
    14881 gcacgtcggt cacgctcgcg gcgcggaccc ggtcgatatc gcgcgcgatg gcggcacgca
    14941 cccgggacag ccgtggctgg gcggcacgct ccagccgctt gtcggcactg cgcaactcgg
    15001 cgagcaatgg ctcggcgctt ccggtcagct gcgcctgctg ctgcgccagc cgcaggcccg
    15061 attcgatatc gaccaccagg ttctcgtccc gggaacgcga gacgctttga atgagttctt
    15121 ccacctgcgc gcgatgcaag gccacctcgc ccagccgagt atcggacagc gcctgccggg
    15181 ccgcggtttc gcgtgccagt tcctgcgcct gcctggccag cgcgcgggct tcgatcgcgt
    15241 tgccgccggc ctcccggctc tgccgcgcaa gctgttcctg gatggtcgaa agcttttgcc
    15301 agagcatcac gctggccgtc agggacagcc cggcgacggc caggaaaaaa taaatcaggc
    15361 ctcgcgccac caacacgcct tgcggccgcg ccctggcctg cgcatcgaca acgtccagcg
    15421 cgggcacctg gctttcgacc gcagggggcg gactcgaggg ctctgaactc atcgaaacga
    15481 ttctagggcc gccaccacag cggccagcga ggggcgtgac tcgcaaacaa caccgaatcc
    15541 cgcccggcgc gcggcctgcg cgatgcgcgg gtgtgtcgcc acggcgcggc cctgcgccca
    15601 gctctcgccg gggagcagcg ttcgcaagtg ttcgatcgcc tgcgagctgc tgaaaagcca
    15661 gacgcagcct tccgtccggc cgcagcaccg cgcctgcgcg agctgcccct ctgtcagcac
    15721 cggcgggcgc cgcaggtacg cgggcaggac ctccacctgg gcccccgccg cggccacccg
    15781 ctcggccagc cagtcgcgcc cgctgctcgc gccctgcgca tccgcgcccc ggacgatcag
    15841 cacgcggtcg ccgtcgccca cctggccggc gacctgctgc cacagcgtct cggaatcgaa
    15901 ctgcgcagcc tgcggtggcg gcgcgtcgat cagctcgggg gtcacccggg ccgccagcaa
    15961 cgcatcgcga gttccgccac ccggcgccca ggcgcgggta ggcgccggcc acggcttgcc
    16021 ctcgggccga tggccgaaga aatgcctgac cgcgttgccg ctgacgaaca tcacggcgcg
    16081 gtagcgatcg agctggcgcc atgccgcgac cagcatggcc ggatcggcga ccggcgcgat
    16141 ttcgatcagc ggcagcgcct gcgcatcaaa acctcggcgg cgcagttcct ccacccagtg
    16201 ccgcgcctcg ctttcgggcc tggtgacgag aacgcgcatg gcgctggggg tgtcagtggg
    16261 cgccggcggc gcgcagttgc tccgccaccg cggcgccgag ttccgccgcc tgcgcgaggt
    16321 ccgccgccgc gacggccgat tgggcgcgca ccagggcggt ggagccctcc ggttcgcccc
    16381 acgctgcgcg caactgcagg tattcgcccg agaacgtggc gaaggccgcc agcggcatcg
    16441 agcagctgcc gcccatggct cgactcaccg cgcgttcggc ggcgaccgcg agccaggtag
    16501 cctggtgcga gagcggcgcc agcagttcga gcaggtcggt gcgatgggcg cgcacctcga
    16561 tgcccagcgc gccctggccc gcggaaggca gcatgtgctc cggctcgaac acgtgccgga
    16621 tccgcgcctc gaggccgagc cgtttcaggc ctgcggcggc caggacaatg gcgtcgtatt
    16681 ggccttcgtc gagcttgcgc aagcgcgtgt cgaggttgcc gcgcagcggt tcgatcttca
    16741 ggtcgggccg cagcgagcgc agcagcacca cgcggcgcag gctggaggtg ccgacgatcg
    16801 cgccttgcgg cagttcggcg aatgtggcga cgcggccgga gacgaaggca tcgcgcggat
    16861 cctcgcgctc catcacgcag cccagggcga agccctgggg gaggtccatc ggtacgtcct
    16921 tgagcgaatg caccgcgagg tcggcatgcc cttcctccag tgccacttcc agttccttga
    16981 cgaacaaacc cttgccgccc accttgctca ggctgcggtc caggatctgg tcgccgcgcg
    17041 tggtcatgcc cagaagggca acctggtgac cgcgttgctc gagcagcgcc ttgacgtgct
    17101 cggcctgcca cagggcaagg cggctttcgc gggtggcgat gactagcttg ctcaacgtcg
    17161 taacctgttt gtaataacgg ggaagagacg atgctagcat gcacgtcctg agaccttgcg
    17221 ggacagatca agaattgctt cagcaatttt gctctgtcct caactgattg agagcgggac
    17281 ggatcttcag ctctgtcctc aactgattga gagcgggacg gatcttcagc tctgtcctca
    17341 actgattaaa caggccccta tctatgccgc caagcccgca atcgccttcg cggcgcgcca
    17401 aggacaacga gcgcccgctg ctggaggaca tccggctgct cggccgcatc ctgggagacg
    17461 tgatccgcga ccaggaaggc gtcgaggcct tcgagctggt cgagcgcatc cgcaagctgt
    17521 cggtgtcttt ccgcagggat gccgacgacg aggccgacaa ggcgctgaaa agcctgctga
    17581 aatcgctgtc gagcgagcag acgatgagcg tgatccgcgc cttcacctac ttcagccacc
    17641 ttgccaacct ggccgaggac cgccatcaca tccgccgccg cgccatccac gagcgcgccg
    17701 gcgataccca ggaaggcagt atcgaggtcg cgctggcgcg gctgcgctgg gccggcatcg
    17761 gcgccaagac ggtttcgcag acgctggcgc acagctacgt gtcgccggtg ctcaccgccc
    17821 accccacgga ggtccagcgc aagagcatcc tcgacgccga gcgcggcatc gccaacctgc
    17881 tgacccagcg cgacgagatc aaggccaatg ccgcgcccaa ggatgcgctg gcgccgcgcg
    17941 aactcgcggc caacgaggcg ggcatcaggg cgcgcgtcct gcaactgtgg caaacgaggc
    18001 tgctgcgctt caccaagctc gcggtggccg acgagatcga aaacgcgctc agctattacg
    18061 agtccacctt cctgcgcgag atcccgaaaa tctacgcgga cctggagcgc gaactgggcc
    18121 atcacccggt cgcggccttc ctgcgcatgg gccagtggat cggcggcgac cgcgacggca
    18181 atccgaacgt gaatgcggga accctggagt acgccctgcg gcggcagtgc gaagtggcgc
    18241 tgcgccacta ccttaccgag gttcattacc tgggcggcga actgtcgttg tcgtcgacgc
    18301 tggtggaagt gacgccggcc atgcgggcgc tcgccgacag ctcaccggac aacaaccagc
    18361 accgcctgga cgagccctat cggcgcgcgc tggccggcat gtactcccgc ctggccgcca
    18421 cgctcaagga attcaccggc ggggacgcgg cgcgccatgc cctgccgccg aagaacccct
    18481 acctgcgcgc cgaggatttc ctggccgacc tgcgcacgat cgaggactcg ctgctggccc
    18541 agcacgcggg ggccctggtc cagcagcgac tgcatccact gatccgcgcg gtggaggtgt
    18601 tcggcttcca cctggccacg gtggacctgc ggcaaagctc cgaccagcac gaggcggtgg
    18661 tggccgaact cctggcggtg gcacgcatcg agccgcatta cgccaggctg gccgaggagg
    18721 cgcggcgcgc tctgctgatg gggctgctgg ccgacgcgcg gccgctgcgg gtgatcggtg
    18781 gccgctactc ggaacacacc cgcgccgaac tggcgatctt cgaaaccgcc aggctgatgc
    18841 gggagcgctt cggcccgcag gcgatccgcc actacatcat cagccacacg gaaaccgcca
    18901 gcgacctgct cgaagtgctg ctgctgcaaa aggaagcggg gctgttgacc gggacaatcg
    18961 atgaccaggc ggtggccgac ctgatcgtgg tgcccctgtt cgagaccatc gaagacctgc
    19021 gcaatgcgcc tgggatcatg cgcgagttct acggcttgcc cggcatcgcc gcgctggtgc
    19081 ggcgttcggg cgccgagcag gacatcatgc tgggctacag cgacagcaac aaggacggcg
    19141 gcatcttcac cagcaactgg gagctgtacc gcgccgagat cgcgctggtc gagctgttcg
    19201 accagctggc cacccgcggc gccgggccgc cccaagacgc gggagcctcc tcgcggggca
    19261 gcgcagcaca cgcggcggca agtggcggcg cgcatggccc gcacgagatc cagctgcgca
    19321 tgttccacgg ccgcggcggt accgtgggcc gcggcggcgg ccccagctac caggccatcc
    19381 tggcgcaacc tcccggcacc gtgcgcggcc agatccgcct aacggaacag ggcgaggtga
    19441 tcggctccaa gtacgcgaac ccggagatcg gccggcgcaa cctggaaacg ctggtcgccg
    19501 ccacgctgga ggcaacgctg ctgcagccga ccaagaccgc gcccaaggcc ttcctggtgg
    19561 cggccgaaac gctctcgcgc accagcatgg ccgcctatcg cgccctggtc tacgagaccc
    19621 ccggttttgc cgattacttt tccggatcca cgccgatccg cgagatcgcg gagctcaaca
    19681 tcggctcgcg cccggcgtcg cgcaagccca gcctgcgcat cgaggacctg cgcgcgatcc
    19741 cctggagctt cagctggggc caatgccggc tgaccttgcc gggctggtac ggcttcggta
    19801 cggccgtgca ccagttcctg cacgagaacc cggcgcatga gcgccgcgcg gccctggccc
    19861 tgctgcagaa gatgtatcgc cagtggccgt tcttcggcgc cctgctgtcc aacatggaca
    19921 tggtgctggc caagagcgac ctggcgctgg cctcgcgcta tgcgcagctc gtggccgatg
    19981 cgcgcctgcg caagcgcatc ttcaccacca tcgaggcgga gtggcatcgc actgtggaag
    20041 cgatgcaggc gatcaccggc gagaaacagc gcctggcaag caacccggcc ctgcagcgct
    20101 cgatccggca ccgtttcccg tacatcgatc ccctgcacca cctgcaggtc gagctggtgc
    20161 gccgctaccg ggaaggcaag atcgacgacc tggcgcggcg cggcatccac atttccatca
    20221 acggcatcgc ggcggcgctg cggaacacgg gttagggcct gttcacactg tttttgccag
    20281 tgtcaggtcc ccagcagctc gcccacgggc ttcaggtcgt cgacgcggtc gcagatggcc
    20341 ttgaccacca tcaggatggg aatgcccagc agcagacccc acacgcccca tagccaaccc
    20401 cacccgagta cgcctatgaa gacggccacg ggattcatgc ggctggcctt gctggtaagc
    20461 caaggcacga ggaggtttcc ctcgatcgta ttgatgagta gcgagatgcc tgccaccgtc
    20521 agcgccatgt tgatcccacc gaactgcagg aaggcgacca gtcccgcggc acctgtcacg
    20581 accactgaac cgatataggg aatgaggttc agcaggccgg cggcaaagcc ccagaccgcg
    20641 gcatgcttga ggcccagcgc ccagaacgcc agccccgtcg tcaccccgac gacgatgctc
    20701 gccagcagct ggaccagcag gtagcgctgg atctgcgccg tgatctcgtc cagcgcctgc
    20761 accgtgatcc tcttgttcgc cagggtgggg ccggtgatct tgaccagctt gcggcggaag
    20821 gtgtctccgg acagcaagag aaagaaggtc aggaacgtga caaccaccac ctggccgatc
    20881 aggctggcca ggcccacggt accgctccag aggtgctcgc ggatgtcgaa ccggggcttt
    20941 tcgatcacca cccgctgcac gccccgcccg gcaggggcag ccgggccggc gtcctgggcc
    21001 gcctgctcga gctgggccgc cgccttttgc actgtttcca ggggcgtatc agtcttgccg
    21061 gtgcgcccgc gcatggaatc gcgcagcttc ttcgcggcag ccggcaggga ctcgatcagc
    21121 tggttggcgt cgtcgctgaa agcatagacc gaggcgcccg tcccgccgag aatgccgaga
    21181 atcagcactg cggcgctgat accccgcgga atgcgccaac gctccagcgc gcggacgatc
    21241 ggcgaaagcg cataactgaa catgaagccc agcatgagcg ggataaaaaa cgcgctggcc
    21301 cagcgcagca tgaccaacac ggccagcacc gtgagcaaca ccagcgccgc gcttcgaaca
    21361 ttcaccggca catgcagcaa cgcatgcgag gggtcgtcag gatcggcggc cagcggcgcg
    21421 gcagccggag cgtcaggttt ttcttccatg gtgcccattg tcggtgccaa tgaacccggt
    21481 caggctgcca gggcctcgcg taccacgctg agctgcctgc gcgaaaccgc cagcacctcg
    21541 tcgatgccgt tcagccgcac cgcccagccc tcgccttctt cggggtcgta gtgtttttcc
    21601 agggcccgga tcgcgtgccg ggccacgagc gcattgcggt gcacgcgcag gaactgcgcc
    21661 gcgtgccgct cttccagctc gctcagcgag gtgtcgagga tatagctctt ggcggcggtg
    21721 cgcaccgtga tgtacttgag ttcggccttg aagtacagca cttccgccag cggcacccgc
    21781 tcggtacgac cgcggtcctg gatcagcagg atttcggacg cgccagcgtg ctgcgcgccc
    21841 cccttggcct gggccagccg ttcgaccttc tgaagtgccg cttgcagacg ctcgagccgc
    21901 acaggcttgg tgaggtagtc cacggcttcc agttcgaacg cggccaccgc atgttccgca
    21961 tgggccgtga tgaataccac ggccggcggc cgcggcagcg acctcagggc ctgggccagg
    22021 gccagcccgt cggcgcccgg catgtggatg tcgagcagca cgacgtcgaa agtcagccgc
    22081 tgcaacagct ccatcgcctg gaccgaattg gaggcctcac cggcgacggc ggccgttggg
    22141 gcgccgcaat cgcccagcag ggtgcgcagg cgcgaacgcg ccagtgcctc gtcgtccacc
    22201 agcaacacct gcaagctcac gccggtacct cgatgcggac ctggtagatc ccatccttca
    22261 gcacggtctg gaagtggcac tggacgtcgt g
//
LOCUS       DASWIY010000420         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3596803, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000420 DASWIY010000000
VERSION     DASWIY010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3596803"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VGA59_14985"
     CDS             complement(<1..1077)
                     /locus_tag="VGA59_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX9721021.1"
                     /translation="MEPNDLNLITPVNIEDEMRDSYMEYAMSVIVGRAIPDVRDGLKP
                     VHRRVLFSMNEQGNHHNRPYRKSARVVGDVIGKYHPHGESAVYDTIVRLAQDFSMRHP
                     LIDGQGNFGSVDGDPPAAMRYTEVRMTQLAAEVLADLDKETVDFQPNYDESLEEPMVL
                     PARIPNLLINGSTGIAVGMASNIPPHNLGESLDAFLHFIDHRDNPSIVELMERMPGPD
                     FPTGAYIMGKQGIYDAYATGRGVITLRAKARVEAEGKGDKEVILVDELPYMVNKARLI
                     EKIAELVRDKRLDGISDLRDESDRKGMRVVVEVKRGFSGEVVLNNLYKMTQLQTSFGI
                     IMLAVVDGQPRVLTLPQFFSHFLSH"
     gene            complement(1472..2569)
                     /locus_tag="VGA59_14990"
     CDS             complement(1472..2569)
                     /locus_tag="VGA59_14990"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9721022.1"
                     /translation="MLERRYRLLWQLAKSLHKTQAKTLVAMAMGLIGCGQMRSFALAG
                     ALARATGIRFKSALKRFYRWVHNGKLDDLACWSALAGRVLPHAGRRPLVAVDWTEWHS
                     NLRVLSAAVCVGARAVPVLVQAFSKTDIPRSQNTRENTFVQLLVRLSAHMQRAVLIFD
                     RGFRRVSLIRELGWLAQPFIIRLAAKVQVVAEGYTGLLSGHPLRPGQRVDLGVCALSQ
                     AKPVRVRIIGVWAAGQKEPWWLATTLTCSARRVIAYYDRRMGIEEHFRDSKGCRYGIK
                     LKWTAFTDPAALARLFLLAAIASAVWLLAGVLACRADPSLRLDTGRKGPRRSLIAVGI
                     DSPQAIATVLALSWGTLRTLWPKARLRDFAW"
     gene            2812..>3291
                     /locus_tag="VGA59_14995"
     CDS             2812..>3291
                     /locus_tag="VGA59_14995"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX9721023.1"
                     /translation="MRRHKRFPYSSSDSTFRSEFMRPWSHRLAWMVCCATVVLLGAGA
                     MVTSTGSSLAVPDWPLAYGQLFPPMVGGILYEHGHRLIAASVGLFTVALAAWLWWAEP
                     RRWVRWLGAAALAIVIVQGLLGGITVLLLLPKAVSIGHALLAQGFFLATVALVQVTAP
                     "
BASE COUNT          484 a         1125 c         1133 g          549 t
ORIGIN      
        1 gtgactgagg aagtgcgaga agaactgggg cagcgtcagc acgcgcggct ggccgtccac
       61 cacggccagc atgatgatgc cgaaggacgt ctgcagctgc gtcatcttgt agaggttgtt
      121 cagcaccacc tcgcccgaga agccgcgctt gacctccacc accacgcgca tgcccttgcg
      181 gtcggactcg tcgcgcaggt ccgagatgcc gtccaggcgc ttgtcgcgca ccagctcggc
      241 gatcttctcg atgagccggg ccttgttgac catgtagggc agctcatcga cgagaatcac
      301 ctccttgtcg cccttgccct ccgcttccac acgcgccttc gcgcgcaggg tgatcacccc
      361 gcggccggtc gcgtacgcat cgtagatgcc ctgcttgccc atgatgtagg cgccggtggg
      421 gaagtccggg ccgggcatgc gctccatcag ctcgacgatg gagggattgt cgcgatggtc
      481 gatgaagtgc agaaacgcat ccaggctctc gcccaggttg tggggcggga tgttgctggc
      541 catgcccacc gcgatgcccg tggaaccgtt gatgagcagg ttggggatgc gcgcgggcag
      601 caccatcggc tcttccagcg actcgtcgta gttgggctgg aagtccaccg tctccttgtc
      661 cagatccgcc agcacctccg cggcgagctg ggtcatgcgc acctcggtgt agcgcatggc
      721 cgccggcggg tcgccgtcca ccgagccgaa gttgccctgc ccgtcgatga gcgggtggcg
      781 catggagaag tcctgcgcca ggcgcacgat ggtgtcgtag accgccgatt ccccgtgcgg
      841 gtggtacttg ccgatcacgt cccccaccac gcgggccgac ttgcggtagg gccggttgtg
      901 gtggttgccc tgctcgttca tggagaacag cacccgccgg tgcaccggct tcagcccgtc
      961 gcgcacatcg gggatggccc gtcccacgat gacgctcatc gcgtactcca tgtaggagtc
     1021 gcgcatttcg tcttcgatgt tgacgggcgt aatcagattc agatcgttgg gctccatcgg
     1081 gctcctgaac tgcgtgaatc ggcgaacggc gaacgggttg gctgcggtgg gatgggtggc
     1141 cgtgccggct ccggtccgat ccccgccggc gaccggatgc gtcccgcggt gggcggcagc
     1201 atcctgccgg cgggtgactc gagcggtggc ggtttggccg cgcgctggcg gcgccccatc
     1261 cggggtgcac cggcggacgc ggcccgccct gcactctccc gcaggctacc acaacggcag
     1321 gggcagggca aaactccagc ccgcgcagat ggccgctccg cgcgggtctg cggggggtcc
     1381 ccggtgtcgg ttttccggtc tgcgccggga cccctcgcag ggtctgggcg ggccgccgcc
     1441 gggccgcgcc gctgaccgct ccccaccttt ttcaccatgc gaagtctctg agacgggctt
     1501 tgggccacag ggtgcgcaat gtgccccaag atagtgcgag cacggtggcg atggcttggg
     1561 gcgagtcgat gccgacggca atgagggaac ggcgcggtcc tttgcgcccg gtgtccaggc
     1621 gcaggctggg atcggcacgg caggccagga cgccggcgag aagccacacg gcgctggcga
     1681 tggctgccag caggaatagc cgcgccagcg cggcaggatc ggtgaaggcg gtccacttga
     1741 gcttgattcc gtagcggcaa cccttgctgt cgcggaagtg ctcttcgatc cccatgcgcc
     1801 ggtcgtagta ggcgatcacg cgacgggccg agcaggtcag cgtggtggcc agccaccagg
     1861 gctccttctg gccggcggcc cacacgccga tgatgcgcac gcggaccggc ttggcctggc
     1921 tcagggcgca cacacccagg tccacccgct gcccgggccg caaggggtgg ccggagagca
     1981 ggccggtata gccctcggcg acgacctgaa ccttggcggc caggcggatg atgaacggct
     2041 gggcgagcca gcccagctcg cggatcaggc tcacgcgccg gaagccccgg tcgaagatca
     2101 gcaccgcgcg ttgcatgtgc gccgagagcc gcaccagcag ttgcacaaag gtgttctcgc
     2161 gcgtgttctg gctgcggggg atgtcggtct tggagaacgc ctgcaccagc acgggcaccg
     2221 cccgtgcgcc cacgcacacc gccgcgctca gcacgcgcaa gttgctgtgc cactcggtcc
     2281 agtccaccgc caccaggggc cgccggcccg cgtggggcag cacccgcccg gccagcgcgc
     2341 tccaacacgc cagatcgtcc agcttgccgt tgtgcaccca gcgatagaag cgcttcagcg
     2401 cgctcttgaa gcgaatgccg gtggcccgcg ccagcgcccc ggccagggca aacgagcgca
     2461 tctgcccgca gccgatcagg cccatcgcca tggccaccaa cgtcttggcc tgcgtcttgt
     2521 gcaggctctt ggcaagctgc cacagcaagc ggtatcgtct ctccagcatg gtgacgcacc
     2581 tccctgtgcg aattcggcta ggaattcaac aatgtgcgtc accatgctcc cgcatttcct
     2641 tcccacggtc cagccacaca cttccgcacc tgccccgcca aaaggtgggg agcggtcagg
     2701 cccggcgtgc attgacgcat ggcggccgag ggcctaagct tagccattaa gcagcgggca
     2761 gcgccgatcc ggcaggggga agcccggggc ggctccagag gtgatgcgag catgcggcgc
     2821 cacaagcgct tcccttactc ttcgtccgac agcaccttcc gttcggaatt catgcggccc
     2881 tggagtcatc gactggcctg gatggtgtgc tgcgccacgg tggtgctgct gggcgcaggg
     2941 gccatggtga ccagcacggg ctcctcgctg gccgtgccgg actggccgct ggcctatggc
     3001 cagctcttcc cgcccatggt gggcggcatc ctctatgagc acggccatcg cctcatcgcc
     3061 gccagcgtgg gcctgttcac cgtcgcgctg gccgcctggc tgtggtgggc cgagccccgc
     3121 cgctgggtgc gctggctggg cgccgcggcg ctggccatcg tgatcgtgca ggggctgctg
     3181 gggggcatca cggtgctgct gctgctgccc aaggccgtct ccatcggcca cgccctgctg
     3241 gcgcagggct tcttcctcgc caccgtggcc ctggtgcagg tgactgcgcc g
//
LOCUS       DASWIY010000421         6375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3257137, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000421 DASWIY010000000
VERSION     DASWIY010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3257137"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..919)
                     /gene="slmA"
                     /locus_tag="VGA59_15000"
     CDS             complement(278..919)
                     /gene="slmA"
                     /locus_tag="VGA59_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEX9721024.1"
                     /translation="MPDAELSTPDSPFSQDVAAPVRKRPKPGERRVQILQALATMLEQ
                     PGAERITTAALAARLDVSEAALYRHFASKAQMFEGLIEFIEQSVFTLVNQIGEREPAG
                     SPQAAKTVAMLLQFAEKNPGMTRVMVGDALVFENERLQQRMNQFFDKIESTLKQCLRS
                     ATEADGSATPSLDAQVRASMITSFVVGRLQRFARSGFKRAPSEHLEACLARML"
     gene            complement(996..2393)
                     /locus_tag="VGA59_15005"
     CDS             complement(996..2393)
                     /locus_tag="VGA59_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9721025.1"
                     /translation="MTSGLRRRLLVMLLAPLILLTLLNAWFDYHSADDAALQQDQRLL
                     ALVPLLADSVIAAGSEGTDPPVLLLAPAVEEFLENGAGHAAYAVVDADGNVVLGESWL
                     AGLPPTTPDPEFHSEENLGVTWRIVRQRQQTVLGEVTIVLADGSDPRQQWARSVLLKV
                     LLPNLMLLAAAAFAVGWAVKRALKPLLELKDAVERRSPRDLSAIDEQASPEEVRPLVI
                     SLNRLFGLVNAQAESQRRFVADAAHQLRTPLAGLQAQVEAWAQAANAANPADGTVRLR
                     TEQVNKLRGATRRTSQLANQLLALSRADPRTMQAQPMQRVDLKDLCEAILETHLDAAT
                     AKRIDLGLDAQAAQAMGHEWLLRELLGNLVDNAVKYTPEGGTVTIRCGPGDGSAFLEV
                     EDDGPGLAPDERLRVLERFYRVRGTQGEGNGLGLAIADEIARVHHSQLELQPGTGGKG
                     LRVSLLLGGKRALGA"
     gene            complement(2464..3165)
                     /locus_tag="VGA59_15010"
     CDS             complement(2464..3165)
                     /locus_tag="VGA59_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9721026.1"
                     /translation="MRLLLVEDDVMVASGIKLGLTDAGYTVDWVGSGERAEEVLRTEA
                     FDAAIIDIGLPRMDGLELTRRLRRPEMASPAMPVLILTARDALRDRVQGLDLGADDYM
                     IKPFELPELLARLRALLRRSQAATTAVLTFGPIELDTANRRACARRNAHVSAIELGPR
                     EWTVLEYLLINAPKPASKDKLLQALTGWDKEITSNAVEVYISRLRSKLEPHGVALRSI
                     RGFGYRLELSDGTSH"
     gene            complement(3393..4283)
                     /gene="argB"
                     /locus_tag="VGA59_15015"
     CDS             complement(3393..4283)
                     /gene="argB"
                     /locus_tag="VGA59_15015"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX9721027.1"
                     /translation="MTDLLNIAPRDKAEILAQALPYIRRFHGKTMVIKYGGNAMTDPE
                     LQADFAEDVVLLKLVGMNPVVVHGGGPQIEAALGRLGKKGQFIQGMRVTDAETMEVVE
                     WVLAGEVQQDIVGLINQAGGKAVGLTGRDGGLIRAQKLKMVDNTDPSKHHDVGQVGDI
                     VAIDPSVVKALQDDQFIPVISPIGFGEENESYNINADVVAGKLAIVLAAEKLLLLTNT
                     PGVLDKTGKLLIDLTAREIDELFADGTISGGMLPKISGALDAARSGVNSVHIIDGRVP
                     HSLLLEILTDQAYGTMIRSH"
     gene            complement(4397..5500)
                     /locus_tag="VGA59_15020"
     CDS             complement(4397..5500)
                     /locus_tag="VGA59_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9721028.1"
                     /translation="MLFLLILCFASAFLASAMLIRYGRGHARRYASTVPQRFHSGNVP
                     RLGGVGMLASCTVGWAWMVVSENQRFPNQIGIDAAAAASLWLAAFLTTAAGVYEDLTQ
                     RLPAGHRLLLTVAAAMVAVWLLELSVPRLGLAGLDAPWSTSIWFGVGLAVVAISGLPH
                     AFNLIDGYNGLAGIVAMVCCFALAYVALQVGDRQLAAMVLALAGATAGFLVWNYPGGL
                     LFAGDGGAYLWGVVIAIASVMLVMQHRQVSPWFPVLLLIYPVWETLFSIYRKTSRGQS
                     PGVADALHFHQLIYRRIVRGVFHDDEARRMLTRNNRTSPYLWGFALLTVTPAVLFWNN
                     TFMLMAFCLLFVISYTYAYCSIVRFKVPRWIRR"
     gene            complement(5519..6289)
                     /locus_tag="VGA59_15025"
     CDS             complement(5519..6289)
                     /locus_tag="VGA59_15025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9721029.1"
                     /translation="MENAHLAGAEPKPGRITLSVVSHGHSGMVERLLLQLRESHGGWI
                     DHCIVTHNVPAPGLPTPEGGWPFRLTQVFNKESAGFGANHNRAFASCASEFFCILNPD
                     VELPGPELWEALVRQAETPGAGLAYPVLLNPDGSRQDNEREMVTPVALLERHLFRRPG
                     RRVDWVSAAFWLVSADVYRRLGGFDESFFMYCEDVDFCLRLRLANWQLRRADVAAVHH
                     AVRRSRWPGRHLAWHVRSLMRLWTGPVLRRYQAVLRAG"
BASE COUNT         1103 a         2184 c         2026 g         1062 t
ORIGIN      
        1 ctcaaccctc cttgcacgtt gatgcccgaa gcagcgagct cggcggacgc tataagacga
       61 caaacctacc cagcgggaag caaaagctca ccaaacaagt caaatgtcgt ccgccaaaac
      121 cagtctctcc cacgggcagg aagcgccaag gggcaaggcg gccacgctgc tcgctagact
      181 ccgaacccca gaaatctaca cggttctggg ggtcaagccc gcaatgacaa acaggaactc
      241 ctggattgcg gctcgaggcc gcaatgacaa tttctcacta aagcatcctc gccagacaag
      301 cttccaagtg ctcggagggc gcgcgcttga agccggaacg ggcgaagcgc tgcagccgcc
      361 cgaccacgaa ggaggtgatc atggaggcgc gcacctgcgc atccaggcta ggcgtggccg
      421 agccgtcggc ttcagtggca ctgcgcaggc attgcttgag cgtggactcg atcttgtcga
      481 agaactggtt catgcgctgc tgcagccgct cgttctcgaa caccagtgcg tcgcccacca
      541 tcacccgcgt catgcccggg ttcttctcgg cgaactgcaa cagcatcgcg acggtcttgg
      601 ccgcttgcgg gctgcctgcc ggttcacgct cgccgatctg gttcacgagc gtgaagacac
      661 tctgctcgat gaactcgatg agcccctcga acatctgcgc cttgctcgcg aaatgccggt
      721 acagcgccgc ttcgctgacg tccaggcggg ccgccagcgc cgccgtcgtg atgcgctcgg
      781 cgcccggctg ctccagcatc gttgccaggg cctgcaggat ctggacccgg cgttccccgg
      841 gcttggggcg cttgcgcacc ggggccgcca cgtcctggga gaagggtgaa tcaggggtgc
      901 tgagttcggc gtcgggcata ggtttcaaat tgtgtgccgt gattctcgca cagccgctcc
      961 tagggtttcc gcctatccat ttcgcctcgc gcgggctacg cgccaagtgc gcgcttaccc
     1021 ccgagcagca gcgaaaccct cagtcccttg ccgcctgttc ccggttgcaa ttcgagctgg
     1081 ctgtggtgca cgcgtgcgat ctcgtcggca atggccagtc ccaggccatt gccttcgccc
     1141 tgggtgccgc gcacacggta aaaacgttcc aggacgcgca gcctttcatc gggtgccagg
     1201 cccggcccgt cgtcctccac ttccaggaaa gcgctgccat cgcccgggcc gcagcggatg
     1261 gtcaccgtgc cgccttccgg cgtgtacttc accgcgttgt ccaccaggtt acccagcagc
     1321 tcgcgcagca gccattcatg gcccatcgcc tgtgcggctt gcgcatccag ccccaggtcg
     1381 attcgcttgg ccgtggccgc gtccagatgc gtttccagga tggcttcgca cagatccttg
     1441 agatcgacgc gctgcatcgg ctgggcctgc atggtgcgag gatccgcccg cgacagcgcc
     1501 agcagctggt tcgccagctg cgacgtccgg cgcgtggcgc cgcgcagctt gttgacctgt
     1561 tccgtgcgca gccgcacggt cccatccgcc gggttcgcgg cattggcggc ctgggcccag
     1621 gcttcgacct gcgcctgcaa accggccagc ggcgtgcgca actggtgcgc cgcatcggcg
     1681 acgaagcggc gctggctctc ggcctgcgcg ttgaccaggc cgaacaggcg gttcagcgaa
     1741 atcaccaggg gccgaacttc ctcgggcgat gcctgctcgt cgatcgcgct caggtcgcgt
     1801 ggcgaccggc gttccaccgc atccttcagt tccagcaacg gcttgagcgc ccgcttcacg
     1861 gcccagccga ccgcgaaagc ggcggcggcc agcagcatca ggttcggcag cagcaccttg
     1921 agcaggacgg agcgtgccca ctgctggcga ggatcggacc catcggccag gacgatggtc
     1981 acttcaccca gcaccgtctg ctggcgctgg cgcacgatac gccaggtgac gcccaggttt
     2041 tcctcgctgt ggaactcggg gtcgggcgtg gtgggcggca ggccggcgag ccaggattca
     2101 ccgagcacga cgttgccgtc cgcgtccacg acggcatagg ccgcgtggcc cgcgccgttc
     2161 tccaggaatt cctcgacggc cggcgcgagc agcaacaccg ggggatcggt gccttcggat
     2221 cccgccgcga tgaccgagtc cgccagcagc ggcaccagcg ccagcagccg ctggtcctgc
     2281 tgcagggccg cgtcgtcggc ggagtgatag tcgaaccagg cattgagaag tgtcagcagg
     2341 atcagcggcg caagcagcat cacgagcagc cggcgcctca gtccggaggt catgccgagc
     2401 gccccgccgc gcccgggggc gggcgcgccg ctgtccatga aatggaagtt caccggcccg
     2461 ttctcagtgg ctggtgccgt cgctgagctc cagccggtag ccgaagccgc ggatcgagcg
     2521 cagcgccacg ccgtgcggct cgagcttgct gcgcaggcgc gatatataca cctccaccgc
     2581 gttggaggtg atttccttgt cccagccggt cagcgcctgc agcaacttgt ccttgctcgc
     2641 cggcttgggc gcgttgatca gcaggtactc cagcaccgtc cactcgcgcg gacccagctc
     2701 gatcgcgctc acgtgagcgt tgcgacgggc gcaggccctg cgattggcgg tgtccagctc
     2761 gatcggcccg aacgtcagca cggccgtggt cgccgcctgc gagcggcgca gcagggcccg
     2821 cagcctggcc agcagttccg gcagctcgaa gggcttgatc atgtagtcgt cggcgcccag
     2881 gtccagcccc tggacgcgat cgcgcagcgc gtcgcgcgcg gtgaggatca gcaccggcat
     2941 cgccgggctg gccatctctg gccggcgcag ccgccgggtc agctccagcc cgtccatgcg
     3001 cggcaggccg atgtcgatga tcgccgcatc gaaagcttcg gtcctgagca cttcctcggc
     3061 gcgctcgccg ctgcccaccc agtcgacggt ataaccggcg tcggtcagcc ccagcttgat
     3121 gccgctggcc accatcacat cgtcttcgac cagtagcagg cgcatagtct cgagattgta
     3181 ttgggccctt gtattgccct cttgtattgc cctcttgtat tgtcccctcc ttgctttgtt
     3241 cctttcaccg tgggagggag ctgcattgtt ccctctcccc tcgggagagg gttagggtga
     3301 gggagaaatc ccatgatcac cttgccggcc gctcgctgcc ctcaccccgc cctctcccag
     3361 agggagaggg agaaaaaccg gaggggggtt catcagtgcg agcggatcat cgtcccgtag
     3421 gcctggtcgg tcaggatctc cagcagcagc gaatgcggca cgcgcccgtc gatgatgtgc
     3481 accgaattca ccccgctcct ggccgcgtcg agcgcgccgc tgatcttggg cagcatgccg
     3541 cccgatatcg tgccgtccgc gaacagctcg tcgatctcgc gcgcggtcag gtcgatcagc
     3601 agcttgccgg tcttgtccag cacgccgggc gtattggtca ggagcagcag tttctcggcc
     3661 gcgagcacga tcgccagctt gcccgccacg acgtcggcgt tgatgttgta gctttcgttt
     3721 tcctcgccaa agccgatcgg gctgatcacg gggatgaact ggtcgtcctg tagcgccttc
     3781 acgacactcg ggtcgatggc cacgatgtcg cccacctggc cgacatcgtg atgcttgctc
     3841 ggatccgtgt tgtcgaccat cttgagcttc tgggcgcgga tcagcccgcc gtcccggccg
     3901 gtgaggccca ccgccttgcc gccggcctgg ttgatcaggc ccacgatgtc ctgctgcact
     3961 tcgccggcca gcacccactc gaccacttcc atggtctcgg cgtcggtcac ccgcatgccc
     4021 tggatgaatt ggcccttctt gcccagccgg cccagggccg cctcgatctg cggtccgccg
     4081 ccgtgcacca ccaccgggtt catgcccacc agcttgagca gcaccacgtc ctcggcgaaa
     4141 tccgcctgca gctcgggatc ggtcatggca ttgccgccgt acttgatcac catggtcttg
     4201 ccatggaagc ggcggatgta aggcagcgcc tgggccagga tttctgcctt gtcgcgcggc
     4261 gcgatgttca ggaggtcggt catgagggcg ggagttcagc gggcgatccc caaattgtgc
     4321 cgcaagcggc cgggccggcc ccgcaatcca ggaagtcatg gatcccgggt caggcccggg
     4381 atgacggact ttgtgcctat cgcctgatcc agcgcgggac cttgaagcgg acgatgctgc
     4441 aataggcgta ggtatacgag atcacgaaca gcaggcagaa cgccatcaac atgaaggtgt
     4501 tgttccagaa cagcacggcc ggcgtcaccg tgagcagcgc aaaaccccac aggtagggcg
     4561 aggtgcggtt gttgcgagtc agcatgcgac gggcttcgtc gtcatggaac accccgcgca
     4621 cgatgcgccg gtagatcagc tggtgaaaat gcagggcatc ggccacgccc ggcgactgcc
     4681 cgcgactggt cttgcggtag atcgaaaaaa gggtttccca cacggggtag atcagcagca
     4741 gcaccggaaa ccacggagaa acctggcgat gctgcatgac cagcatcacg ctggcgatag
     4801 cgatcaccac gccccagaga taagcgccgc catctccggc aaacagcagc ccgccgggat
     4861 aattccacac caggaaaccc gccgtcgcgc ccgcgagcgc cagcaccatg gctgccaatt
     4921 gccggtcgcc gacctgaagg gccacatagg ccagcgcgaa gcagcacacc atcgccacga
     4981 tacccgccag gccgttatag ccgtcgatca ggttgaacgc atggggcagg ccgctgatgg
     5041 caaccaccgc aagccccacg ccaaaccaga tgctcgtgga ccaaggggcg tcgaggcccg
     5101 ccaggcccag gcgcggtacc gacaactcca gcaaccacac cgcgaccatt gccgcggcca
     5161 ccgtcaacag caggcggtga ccagcgggca ggcgctgggt aaggtcctcg taaacgccgg
     5221 ctgccgtggt gagaaacgcc gccagccaca gggatgcggc ggcagcggcg tcgattccga
     5281 tctggttcgg aaacctctgg ttttcggaca cgaccatcca ggcccatcca acggtgcacg
     5341 aagccagcat accgacaccg cccagccgtg gcacgttgcc cgagtgaaat cgctggggca
     5401 cggtgctggc gtaccggcgc gcgtgcccgc ggccgtaacg gatcagcatc gcgctggcca
     5461 ggaatgcgct ggcaaagcac aggatcagca gaaagagcat gcggcagcgg ctcggacttc
     5521 agccggccct caggacggcc tggtagcgcc gcaacacggg ccccgtccaa aggcgcatca
     5581 agctgcgcac atgccaggcc agatgccggc ccggccagcg gctgcgccgc acggcgtggt
     5641 gcactgccgc cacgtcggcc cgcctgagct gccagttggc cagccgcagc cggaggcaga
     5701 aatcgacgtc ttcgcaatac atgaaaaaac tctcgtcgaa cccgcccagc cggcggtaca
     5761 catcggccga caccagccag aacgccgcgc tgacccaatc gacacggcgc cccgggcgcc
     5821 tgaataaatg gcgctccagc agcgcgacgg gagtcaccat ttcgcgctcg ttgtcctgcc
     5881 ggctgccgtc cgggttcagg agcacggggt aggccagacc ggcgccgggg gtttcggcct
     5941 gccgcaccag tgcctcccat agttcggggc ccggcaactc gacgtcggga ttcaggatac
     6001 agaagaactc cgaagcacaa ctcgcgaagg cgcgattgtg gttagcgccg aatccggccg
     6061 attccttgtt gaacacctgg gtcaagcgga agggccagcc gccctcaggc gtcggcagcc
     6121 ccggcgccgg cacgttgtgg gtcacaatgc aatggtcgat ccagcctcca tggctctcgc
     6181 gcagctgaag cagcaggcgc tccaccatgc cgctgtggcc atggctcacg acggacaggg
     6241 tgatacgccc gggcttgggc tccgcacctg caaggtgggc attttccatt gaggggattc
     6301 gattcgctcg ctgctgaacc gctcattcta atctgggggg gtcgaggcgc cccgatccgc
     6361 gttgtccctc ccttg
//
LOCUS       DASWIY010000422         6589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_663381, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000422 DASWIY010000000
VERSION     DASWIY010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663381"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VGA59_15030"
     CDS             complement(<1..426)
                     /locus_tag="VGA59_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX9721030.1"
                     /translation="MKHPTKAELSKARVLVVGDPMLDRYWHGAVDRISPEAPVPVVKV
                     TREEERMGAAANVAYNIVTLGARASFLGVVGDDEPGHRLEALLTGTGIATHLKRDPGL
                     RTTVKLRVIGRNQQLLRLDFENEPDHEALASQAETFARLA"
     gene            complement(560..1693)
                     /gene="lapB"
                     /locus_tag="VGA59_15035"
     CDS             complement(560..1693)
                     /gene="lapB"
                     /locus_tag="VGA59_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEX9721031.1"
                     /translation="MDFDLSWVLWGLPIAFVLGWLASRLDLRQLRIENRQAPKAYFRG
                     LNFLLNEQQDQAIDAFIEAVQNDPDTSELHFALGNLFRRRGEYERAVRVHEHLLSRGD
                     LSRADRHRAQHALALDFLRAGLLDRAEEALRKLEGTAFEGQALLALLAIYERSRDWVQ
                     ASETARKLDASGQGNFSGRLAHYLCEQAANAPPAQAEQLLRQAMKTAPGAARPGIDLA
                     ALQRRAGDAEGAFKTLSGLAHSAPSAIPLVARPLTEAAIAAGRQAQALEILHTSYELS
                     PSLDVLEGIVALEPDAAVAGDWYVKHLEKEPSLIAASRWLAGEKLEREQFHPHVQAAL
                     EHAVKPLTRYRCAACGFEAKQHFWQCPGCQAWDSYPARRVEEL"
     gene            complement(1683..1970)
                     /locus_tag="VGA59_15040"
     CDS             complement(1683..1970)
                     /locus_tag="VGA59_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX9721032.1"
                     /translation="MKYLMWLLKAAIFFTLFAFALNNQQDVTVLFFFGTQWRAPLVLV
                     VLAAFALGIAIGALGMVPRWWRHRAAAQRAQQGMAMPQTAGEAGTTPNHGL"
     gene            complement(2021..2332)
                     /locus_tag="VGA59_15045"
     CDS             complement(2021..2332)
                     /locus_tag="VGA59_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX9721033.1"
                     /translation="MTRSDLVEELAARFSQLTHRDAEYAVKTILDAMSEALVRGHRIE
                     IRGFGSFSINRRPPRMGRNPRSGESVHIPEKRVPHFKPGKALREAVDRRTAEIEANGK
                     A"
     gene            complement(2497..4182)
                     /gene="rpsA"
                     /locus_tag="VGA59_15050"
     CDS             complement(2497..4182)
                     /gene="rpsA"
                     /locus_tag="VGA59_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX9721034.1"
                     /translation="MSESFAALFEESLQRSEMRTGEVITAEVVRIEHSFVVVNAGLKS
                     EAYVPIDEFKNDKGEIEVQVGDFVSVAIDAIENGYGDTILSRDKAKRLASWMSLEKAL
                     ESGEFVTGTTSGKVKGGLTVLVNGIRAFLPGSLIDTRPIKDLTPYENKTMEFKVIKLD
                     RKRNNVVLSRRAVVEASMGEERAKLMETLKEGSVVRGVVKNITEYGAFVDLGGIDGLL
                     HITDMAWRRVRHPSEVVQAGQEITAKILKFDTEKNRVSLGLKQMGDDPWMGVNRRYPQ
                     GTRMFGKITNIADYGAFVELEPGIEGLVHVSEMDWTNKNVAPSKIVSLGDEVEVMILE
                     IDEDKRRISLGMKQCKANPWQEFAQNTKRGDRVKGPIKSITDFGVFVGLAAGIDGLVH
                     LSDLSWNEPGEAAVRNYKKGQEVEAIVLAVDVDRERISLGIKQLDADPFTTFVSVHDK
                     GSAVSGRVKTVDPKGAEIDLGQDIIGYLRASEISRDRVEDARNVLKEGDEVHAVVVNI
                     DRKTRNIQLSVKAKDMADQQEQMSSLSQQSARENAGTTSLGALLRAKLDNTEK"
     gene            complement(4344..6332)
                     /locus_tag="VGA59_15055"
     CDS             complement(4344..6332)
                     /locus_tag="VGA59_15055"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HEX9721035.1"
                     /translation="MFSTEFLDIPPLQGAAGTLPLPGSKSISNRVLLLAALSGGTTTV
                     HDLLDSDDTRVMLDALRTLGCGVRHSGAAVEITGLGGRAPASHARLFLGNAGTAMRPL
                     AAALAVIGGEFELSGVARMHERPIGDLVDALRRLGCDIEYLGVAGYPPLRIGRPRLSL
                     AAPILVRGDVSSQFLTALLMALPLVARQDVVIEVVGALISKPYIEITLNLLARFGIAV
                     RREGWQRFVIPAGSSYRSPGSIHVEADASSASYFIALGAIAAQPPRPVRIAGVGLDSI
                     QGDIRFVEAARLMGADVRGGPGWLEVSRGAWPLKAIDLDCNHIPDAAMTLAIMALYAD
                     GPTTLRNIASWRVKETDRLAAMATEFRKLGAVVGEGPDFIRIDPPQNWKAATINTYDD
                     HRIAMCFSLAAFNPAGVPVRIQDPKCVAKTFPDYFETLFSVVQTPAARIPVICVDGPT
                     ASGKGTLASQVAQKLGYHYLDSGALYRITALAAVRAGLRLDLAHEHGIARMAQTLPVR
                     FQAGQVLLGEQDVTDAIRTEEAGMNASRVSALPAVRTALVALQHGFRRLPGLVADGRD
                     MGTVIFPDAQLKVYLTASAAQRAQRRHKQLISKGISTTIDALRQDLEARDASDSSRAV
                     APLKPAEDALLLDNSSQSVEESVAQVLRWWEGKQPFAA"
     gene            complement(6358..>6589)
                     /locus_tag="VGA59_15060"
     CDS             complement(6358..>6589)
                     /locus_tag="VGA59_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX9721036.1"
                     /translation="GPGFRDFTRIAASDPKMWRDILLANRHELLAQAQIFQRTLNQME
                     LLIESGDGEALEGLLAQASGARAQWRMGASKK"
BASE COUNT         1047 a         2161 c         2173 g         1208 t
ORIGIN      
        1 cgccagccgg gcgaaagttt cggcctgtga agccagcgct tcgtgatcgg gctcgttctc
       61 gaagtccagt cgcagcagct gctggttgcg cccgatcacg cgcagcttca cggtcgtacg
      121 caatccggga tcgcgcttga ggtgcgtcgc gattcccgta cccgtcagca gcgcctcgag
      181 ccgatggccg ggctcgtcgt cgccgacgac gcccaggaag ctggcccgtg cgcccagcgt
      241 gacgatgttg tacgccacgt tcgcggcggc gcccatacgc tcttcctcgc gcgtgacctt
      301 caccacgggg acgggcgctt ccggcgatat ccggtccacg gcgccgtgcc aataccgatc
      361 gagcatcggg tcgccgacga ccagcacacg tgctttcgag agttctgcct tcgtcgggtg
      421 tttcatagtc gaatattgcg tcatttggca agtgcacttg tagtcgcgcg gcccagtcgt
      481 gggccaccgc ggatccggct tcgccggtcc gctggtggcg ccccttgagg ggaggcgggc
      541 gtagcccgct tcggggtggt cacaactctt ccacccgccg cgccggatag ctgtcccagg
      601 cctggcagcc ggggcattgc cagaaatgct gctttgcctc gaagccgcag gcggcgcagc
      661 ggtacctcgt cagcggcttc accgcatgtt caagggccgc ttggacatgc ggatgaaact
      721 gttcgcgttc cagcttttcg ccggccagcc acctggaggc ggcgatcagg gacggctctt
      781 tctccaggtg cttcacatac cagtccccgg ccactgcggc gtcgggctcg agcgcgacaa
      841 ttccctccag cacgtccagc gacggggaca gctcgtagct ggtgtgcagg atttccagcg
      901 cctgggcctg ccggccggcg gcaatggcgg cttcggtgag gggcctggcc accagtggaa
      961 tggccgacgg agcggagtgg gccaggccgg acaaagtctt gaaggctccc tcggcgtcgc
     1021 cggcgcggcg ctggagcgcc gcgaggtcga tgcccggacg cgccgcccca ggggctgtct
     1081 tcatcgcctg gcgcaacagc tgttcggcct gcgccggcgg ggcattggcg gcctgctcgc
     1141 aaaggtaatg cgccagccgc ccgctgaagt tgccctggcc cgatgcgtcg agtttcctgg
     1201 cggtctccga cgcctgaacc cagtcgcgag accgctcgta gatcgccagc agcgcgagca
     1261 gcgcctggcc ctcgaacgcc gtgccttcca gcttgcgcag cgcttcctcg gcccggtcga
     1321 gaaggccggc cctgaggaag tccagcgcca gcgcgtgctg ggcgcgatgc cggtcggcgc
     1381 ggctcaagtc gccgcgcgaa agcaggtgct cgtgcacccg caccgcgcgc tcgtattcgc
     1441 cgcggcggcg aaacaggttg cccagggcga agtgcagctc cgaggtgtcc ggatcgttct
     1501 gtaccgcctc gatgaaggcg tcgatggcct ggtcctgctg ttcattgagc aggaaattga
     1561 gtccccggaa ataggccttg ggcgcctgcc ggttttcgat gcgcaactgg cgcaagtcaa
     1621 gccgcgaggc cagccagccc agcacgaaag cgatcggcag gccccacaga acccagctca
     1681 ggtcaaagtc catggttcgg cgtggtgccc gcctcgccgg cggtttgcgg catcgccatg
     1741 ccctgttgcg cgcgctgcgc cgcggcgcgg tgcctccacc agcggggcac catgccgagc
     1801 gcgcctatcg cgatgcccag cgcgaaggca gccagcacca ccagcaccag gggcgcgcgc
     1861 cactgcgtac cgaagaagaa aagtaccgtg acgtcctgct ggttgttcag cgcgaaggcg
     1921 aacagggtaa aaaaaatggc tgccttcagc agccacatga ggtatttcat cgaactcctc
     1981 agtggagaat cgattctagt gccacagccc ggcagtaagg tcaggccttg ccgttcgctt
     2041 cgatctccgc cgtacgcctg tcgaccgcct cgcgcagcgc cttgcccggc ttgaagtgcg
     2101 gcacgcgctt ctcggggatg tggacgcttt cgccgctgcg cgggttgcgg cccatgcgcg
     2161 gcgggcgccg gttgatcgaa aagctgccga agccacggat ttcgatgcgg tgcccgcgca
     2221 ccagtgcttc gctcatcgcg tcaagaatgg tcttgacggc gtattccgcg tcgcggtgcg
     2281 tgagctggct gaagcgggcg gccagttctt cgacgagatc ggaacgtgtc ataggtcttg
     2341 gggggcttgt catcccgggc tagacccggg atccatgcct ttggagtcgc ggatgccccc
     2401 ggaaacgcag tccggggcag gctcctcaag tccggcttga cggctctttc ggcatggatt
     2461 gcgggtcaag cccgcaatga cagtcctttt gtcttcttac ttctctgtgt tatccaattt
     2521 cgcgcgcagc agggcgccca ggctggtcgt gccggcgttc tcacgtgccg actgctggga
     2581 cagactggac atctgttcct gctggtcggc catgtccttg gccttgacgg acagctggat
     2641 gttgcgcgtc ttgcggtcga tgttcacgac cacggcgtgc acctcgtcgc cttccttgag
     2701 cacgttgcgg gcatcttcca cgcggtcgcg tgagatttcg ctggcacgca ggtagccgat
     2761 gatgtcctgg cccaggtcga tctcggcgcc cttggggtcg acggtcttga ccctaccgga
     2821 gacggcggaa cccttgtcgt gcaccgacac gaaggtggtg aacgggtcgg cgtccagctg
     2881 cttgatgccc aggctgatgc gctcgcggtc cacgtcgacg gccagcacga tcgcttcgac
     2941 ttcctggccc ttcttgtaat tgcgcacggc ggcttcgccg ggctcgttcc aggacaggtc
     3001 ggacaggtgc accaggccgt cgatgccggc ggccaggccg acgaacacgc cgaagtcggt
     3061 gatcgacttg atcgggccct tgacgcggtc accgcgcttg gtgttctgcg cgaattcctg
     3121 ccacgggttg gccttgcact gcttcatgcc caggctgatg cggcgcttgt cttcgtcgat
     3181 ctccaggatc atgacttcga cttcgtcacc cagcgagacg atcttgctgg gggcgacgtt
     3241 cttgttggtc cagtccatct cggagacgtg caccaggcct tcgatgccgg gctcgagttc
     3301 cacgaacgcg ccatagtcgg cgatgttggt gatcttgccg aacatgcgcg tgccctgcgg
     3361 gtagcggcgg ttcacgccca tccacgggtc gtcgcccatt tgcttcaggc ccagcgacac
     3421 gcggttcttc tcggtgtcga acttgaggat cttggcggtg atttcctggc cggcctgaac
     3481 cacttcgctc gggtggcgca cacgcctcca ggccatgtcg gtgatgtgca gcaggccgtc
     3541 gataccgccg aggtccacga acgcaccgta ttcggtgatg ttcttgacca cgcccctgac
     3601 cacggagcct tccttcagcg tttccatcag cttggcgcgc tcttcgccca tcgaggcttc
     3661 gaccacggcg cggcggctca gcaccacatt gttgcgcttg cggtcaagct tgatgacctt
     3721 gaactccatc gtcttgtttt cgtacggcgt caggtccttg atcggacgcg tgtcgatcag
     3781 cgagccgggc aggaacgcgc ggatgccgtt gaccaggacg gtgaggccgc ccttgacctt
     3841 gccgctggtc gtgccggtga cgaattcgcc ggactccagt gccttctcga ggctcatcca
     3901 tgatgcgagg cgcttggcct tatcgcgcga caggatggtg tcgccataac cattctcgat
     3961 ggcgtcgatc gccaccgaca cgaagtctcc aacctggact tcgatttcgc ccttgtcgtt
     4021 cttgaactcg tcgatgggca cgtaggcttc ggacttgagg ccggcattca cgaccacgaa
     4081 gctgtgttcg atacgcacca cttcggcggt gatcacttcg ccggtacgca tttcggagcg
     4141 ttgcagcgat tcttcaaaca gggcggcaaa agattcagac attaaggttt ccttgcacgt
     4201 cgcacaggtt tccatccgct atgagcaaaa tcgcgagcgg ctgtttctag gactgggacg
     4261 gcggtttggt gcggcacggg ccgcgggtta ggttgacgaa ccacactgcc tgggcgctgt
     4321 cgcgcgagaa gggcggcgtg gagctaggcg gcaaacggtt gtttgccttc ccaccaccgg
     4381 agcacctgcg ccaccgattc ttcaaccgac tggctcgagt tatccaggag gagggcatct
     4441 tcagcgggct tcaggggcgc taccgcgcgg gacgaatcgc tcgcatcgcg cgcttccagg
     4501 tcctgccgaa gagcgtcaat tgtagttgaa ataccctttg aaatcaattg cttatggcgg
     4561 cgctgcgcgc gctgcgcggc gctggccgtc aggtagacct tcagctgcgc atccgggaag
     4621 atcacggtgc ccatgtcgcg cccatcggcc accagcccgg gcaggcgccg gaagccgtgt
     4681 tgcaaagcca ccaacgccgt gcgcacggcg ggcagcgcgg aaacccggga ggcgttcatg
     4741 cccgcctctt cggtgcgtat cgcgtccgta acgtcctgct cgcccagcag cacctggcca
     4801 gcctggaaac gcaccggcag cgtctgggcc atccgggcga tgccgtgctc gtgcgcaagg
     4861 tccagccgca ggccggcccg cacggcggcc agggccgtga tgcgatacaa ggcgccggaa
     4921 tccaggtagt ggtagcccag tttttgggcg acctgcgagg ccagcgtgcc cttgccggaa
     4981 gcggtcggac cgtccacaca gatcacggga atgcgcgccg ccggcgtttg caccaccgaa
     5041 aacagcgtct cgaagtaatc gggaaacgtc ttggccacgc atttcgggtc ctggattctc
     5101 accggcacgc cggccgggtt gaatgccgcc agcgagaagc acatggcgat gcggtggtcg
     5161 tcataggtat tgatggttgc cgccttccag ttctgcggcg ggtcgatccg gatgaagtcg
     5221 ggaccctcac cgaccacggc gccgagcttg cgaaattcag tggccatggc cgcgagccgg
     5281 tcggtttcct tgacgcgcca gctggcgatg ttgcgcagcg tggtcgggcc atcggcgtac
     5341 agggccatga tggccagcgt catcgccgca tccggaatat ggttgcagtc caggtcgatg
     5401 gccttcaggg gccaggcgcc acgggaaacc tccagccagc cggggccgcc gcgcacgtcg
     5461 gcgcccatca gccgcgcggc ttcgacgaag cggatgtcgc cctggatcga gtccaggccg
     5521 acgcccgcga tgcgcaccgg ccgcggcggc tgcgccgcga tcgcgccaag cgcgatgaaa
     5581 tagctcgcgg acgaggcatc cgcttccaca tggatgctgc ccggcgaccg gtagctgctg
     5641 ccggcgggaa tgacgaaacg ctgccagcct tcgcgccgca ccgcgatgcc gaaacgcgcc
     5701 agcaggttca gcgtgatctc gatgtagggc ttggagatca gcgcgccgac cacttcgatg
     5761 accacatcct gccgggctac gagtggcaga gccatcagca aggcggtgag gaactggctg
     5821 gagacgtcgc cgcgcaccag gatcggcgcg gccagcgaca gccggggccg gccgatgcgc
     5881 agcggcggat agccggccac gcccaggtat tcgatgtcgc agcccagccg gcgcaatgcg
     5941 tcgaccagat cgccgatggg cctctcgtgc atgcgcgcca cgccgctcaa ctcgaactcg
     6001 ccgccgatca cggccagggc ggccgccagg ggccgcatcg cggttccggc gttgcccagg
     6061 aacagcctgg catgcgacgc cggcgcccgg ccgcccaggc cggtgatctc cacggctgcg
     6121 ccgctgtgcc tgacgccgca gcccagggtg cgcaaggcat cgagcatgac ccgggtgtcg
     6181 tccgagtcga gcaggtcgtg gaccgtggtc gttccgccgc tcaacgcggc cagcagcagc
     6241 acgcggttgg aaatgctctt ggaaccgggc agcggcaaag ttcccgcggc gccctgaagg
     6301 ggcgggatgt cgaggaattc ggtggagaac atcaggcctg gccccgcggg gtccggatca
     6361 tttcttcgac gcacccattc gccactgcgc gcgcgcgccg ctcgcctggg cgagcagccc
     6421 ctccagcgcc tcgccgtcgc cgctttcgat cagcagttcc atctggttca aggtgcgctg
     6481 gaaaatctga gcctgggcca gcagttcgtg cctgttggcc agaaggatgt cgcgccacat
     6541 cttgggatcg ctggccgcga tccgcgtgaa atcgcgaaag cccggaccg
//
LOCUS       DASWIY010000423        11975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3259094, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000423 DASWIY010000000
VERSION     DASWIY010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11975
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3259094"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..2042)
                     /gene="kefC"
                     /locus_tag="VGA59_15065"
     CDS             complement(207..2042)
                     /gene="kefC"
                     /locus_tag="VGA59_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HEX9721037.1"
                     /translation="MEHAPAWLTNSLIYLGAAVIAVPLSRALGLGSIIGYLAAGIAIG
                     PWGLGLVSNVEDILHFAEFGVVLMLFLVGLELEPRRLWSLRRPIFGSGSAQVLGCAAL
                     LWAAGMAAGLGWRVSLVAALGLALSSTAISLQVMGERNLLPTSSGQAGLSILLFQDVA
                     AIPIIALLPLLGGMAAETSAASRWLEGAKILAVIGGVILGGRLLLRPLLRWIAKSRTP
                     EIFTAASLLLVVAVAALMQLAGLSMALGAFLAGVLLAESEYRRELETDIEPFKGLLLG
                     LFFIAVGMSIDFAVLLESPGLMALIVVGFLALKAALIHALARGIGLPVQERPVFTLLL
                     AQGGEFAFVVFQAAAGARVIPAEISSLLIGAVAVSMLLTPLLLVAIDKLVLPRYANCN
                     TPRLEEISEPQHAPIIIAGFGRYGQIVGRMLAAQGIAATVLDHDADMIEAARAFGYKV
                     FYGDATRLDLLRTAGAATAKVLVVAVDDTGQSLAIVDVAREHFPHLELVARARDVTHW
                     NELRDRGVARVEREVFEASLRSARTVLEVLGFAPHEARAHAMRFRRHNLQLFEQMYPH
                     HKDRAKVIAVIKQGRRQLEEQMAQERAEREQRRRDGQERPPGWDN"
     gene            complement(2054..2755)
                     /locus_tag="VGA59_15070"
     CDS             complement(2054..2755)
                     /locus_tag="VGA59_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX9721038.1"
                     /translation="MDEIAKEEPSRGVLVLAAHPNWRESRVNRRLVAAARETTGVQVQ
                     DLYGSYPDYDIDVAAEQARAADASLIVLLHPIQWYSMPALLKLWLDEVLTYGWAYGPG
                     GTALRGKDLWLVATTGGPESSYHPQSYNRYFFDAFLPPYEQTAALCGMRFLPPLLLHG
                     AHRASEQSVADHVAVFGERLRSYPQWPELDELGECPACDVPPTDRPRTGTDVIPDLIR
                     DPSRRATVDSGSSPE"
     gene            2827..3444
                     /locus_tag="VGA59_15075"
     CDS             2827..3444
                     /locus_tag="VGA59_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9721039.1"
                     /translation="MPVVVVANPKGGVGKSTLATNLAGFLASKGHAVMLGDADRQQSS
                     RLWLGLRPNGARPISTWQLSEGNIVKPPKGTTHVVLDTPAGMHGKVFKDVLQLADRVI
                     VPLQPSIFDIFATRAFLDELAEHRKASKLQVGIVGMRVDARTIAAEKLHEFVDALGLP
                     VLGYLRHTQNYIHLAARGLTLFDVAPGRVERDLAQWEGICRWTDS"
     gene            3525..3980
                     /locus_tag="VGA59_15080"
     CDS             3525..3980
                     /locus_tag="VGA59_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9721040.1"
                     /translation="MKTLQHLSELPALVGQEIAVSDWITITQEQVNLFAQATGDHQWI
                     HVDVEKAKAGPFGAPIAHGFLTLSLIPKFFQTSFEILDSRMGVNYGLNKVRFTAPVPV
                     GSRLRAHMKLLVCEPIENNGQQMAWLVTVEREGSDKPVCIAESLSRRYP"
     gene            complement(4038..4433)
                     /locus_tag="VGA59_15085"
     CDS             complement(4038..4433)
                     /locus_tag="VGA59_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX9721041.1"
                     /translation="MEHTDHPHAAPAGEHPGAHAQGQQHPLGIYFKIWALLFVLSAAS
                     YMVDYMHVQGLLRWTLIIVFMLLKAGLIVSVFMHMLWERLALVYAILVPPLLLMVLLG
                     IGALEADYTLLTRGAFFAPEIKQPAPPGH"
     gene            complement(4450..5172)
                     /locus_tag="VGA59_15090"
     CDS             complement(4450..5172)
                     /locus_tag="VGA59_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III family protein"
                     /protein_id="HEX9721042.1"
                     /translation="MATHDATGTAAPLPRPGPWQSIVADVSSDQRAFKGVPWGKPMMW
                     IFLLSDTFIFGSFLISYMTVRASTTVPWPNTSEVFSLMMFGRNVPLLLIAIMTFVLIS
                     SSGTMALAVNFGYRRDRKTTFMLLIATAALGATFVGMQAFEWYKLISEGVRPWGNPWG
                     AAQFGSSFFMITGFHGTHVTIGVIFLIIVAVKVLRGDLDQERPGFLTGRRGNYEIVEI
                     LGLYWHFVDLVWVFIFAFFYLW"
     gene            complement(5190..5888)
                     /locus_tag="VGA59_15095"
     CDS             complement(5190..5888)
                     /locus_tag="VGA59_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX9721043.1"
                     /translation="MTIGLVFLSVLMAVIVWWLFRQTINVQPWQAQVAVPDVGGDAAA
                     PPAAKMALWVFLAVATSLFVLFVSAYAMRLGLADWTPLPRPRLLMLNTALLVGASLAM
                     EWTVHAARRADRDNLRRGLWASGVLTFGFLAGQLFVWKQLNDAGYFVSSSAATAFFYL
                     LTAVHGLHVLGGLVAWARASARAWRGTDPARIRLGVELCATYWHYLLAVWVVLYALLV
                     SEGVGLAICSSTPL"
     gene            complement(5893..7692)
                     /locus_tag="VGA59_15100"
     CDS             complement(5893..7692)
                     /locus_tag="VGA59_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9721044.1"
                     /translation="MTTYVAHEESQFAPPAEVGEAELYHPKTFIGKYIWSQDAKVIAI
                     QYAVTAIAIGLVALVASWLMRLQLGFPGAVGFIDPSTYLQLVSMHGMIMVIYLLTALF
                     LGGFGNYLIPLMVGARDMVFPYVNMVSYWLYLLAVLLLVASFFVPGGASGAGWTLYPP
                     QAILDGTPGAQWGIILLLVSLGVFVIGFTMGGLNYVVTVLQARTRGMTMMRLPLTVWG
                     IFMATILALLAFPALFVSAIMMTLDLTLGTSFFMPALVSKGQQLSYSGGNPLLFQHLF
                     WFFGHPEVYIVALPAFGIVSDLISTHARKNIFGYRMMVWAILVIGGLSFIVWAHHMFV
                     SGMNPYFGFFFATTTLIIAVPTAIKVYNWVLTLWRGNIHLTVPMLFAIAFIFTFVNGG
                     LTGLFLGNVVVDMPLSDTMFVVAHFHMVMGIAPVLTVFGAIYHWYPKITGRMLDDTLG
                     KIHFWITFIGTYAIFYPMHYLGFMGIPRRYYAITGTNFIPDSAHLLNAWISLAAFVVG
                     AAQIVFLYNLAASYFRGRPAGNNPWNATTLEWQTAHTPPRHGNFGKELPAVYRWAYDY
                     GVPGLKTDFIPQNVAPSDVPAEAWEKTPQPTGG"
     gene            complement(7709..9061)
                     /locus_tag="VGA59_15105"
     CDS             complement(7709..9061)
                     /locus_tag="VGA59_15105"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9721045.1"
                     /translation="MPIAIALILLALGSVLFHFLSPWYFTPIASNWGTIDNTISITFW
                     VTGVVFVAVSLFMAMAVIRYRHREGGRAAYEPENKKLEWWLLALTGVGVAAMLAPGLL
                     VWADVIHVPKDARVAEVMAQQWHWSYRLPGKDGKLGTVHSRFVNDANPFGMNPADPNG
                     HDDVLLASPELHVPLGQPVKLLLRSKDVLHNFSVAQLRVKMDMVPGLVTYIWFTPTRT
                     GSFDLLCEELCGIGHFAMRGRIVVDEPGAFQAWLDRQSTFAQAQTRPAGNAVTGKASY
                     AACAACHGANGEGNVALNAPRLSGQGAWYLERQLRLFKQGARGTHDKDVFGKMMAPMA
                     ATLADDSAIANVAAYIASLPDAPVTTTIKGNVDKGRQRYATCAACHGADGRGIAATNA
                     PRLQGMSDWYMATQLKNFRDGVRGAHAQDIYGGQMALIAGMLSDDAAIGDILAYINSR
                     "
     gene            complement(9148..9663)
                     /locus_tag="VGA59_15110"
     CDS             complement(9148..9663)
                     /locus_tag="VGA59_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013951778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX9721046.1"
                     /translation="MKQRIYWLLPDLASAGRTMAGLRGAGIESRHMHFAARDGLDLTG
                     LHAANVLQTSDVIHAAQRGLVFGGATGICTGLLAALFFPIVGDAPQWEVAGILAVFGG
                     LFGAWSSSMIGISIPSPRLVRFESAIAQGRILLMVDVPYRQAQAIASLLRAAHPEAHF
                     EGVDRLSPVFP"
     gene            complement(9929..10417)
                     /locus_tag="VGA59_15115"
     CDS             complement(9929..10417)
                     /locus_tag="VGA59_15115"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEX9721047.1"
                     /translation="MFHIVLVEPEIPPNTGNVIRLAANTGCTLHLIEPLGFSMDDKHL
                     RRAGLDYHEYADVRCHAGWQRFLDTQQPDPLRLFALTTRGARLVHETAFRPGDWLVFG
                     AETRGLAPALRDSLPPGQRLKLPMRPGQRSLNLSNAVAVTVYEAWRQNGFGPRESPAP
                     LS"
     gene            complement(10451..11149)
                     /locus_tag="VGA59_15120"
     CDS             complement(10451..11149)
                     /locus_tag="VGA59_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX9721048.1"
                     /translation="MRRLTDRLAAALPGQCAVCRAWPAQPVCEACVARFAQPQARCRR
                     CALPVPAGVAECGHCVGAPPPLDGCHAAVSYEFPWPALIAQYKFHGQAGWARSFATLM
                     RSAPWVEPALDEADLVLPMPLSRARLAERGFNQALLLARALAPRKTVPGLLLRVRHTA
                     AQAGLDRADRLGNVEGVFAVDPLPATSVRGRSVVLVDDVMTSGASLHAAAQTLRQAGA
                     ARITAVVLARTSDR"
     gene            11258..>11975
                     /locus_tag="VGA59_15125"
     CDS             11258..>11975
                     /locus_tag="VGA59_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HEX9721049.1"
                     /translation="MTDERPPTIDPVAAARWGRVAPARSPWLHEEVARRMEDRLQWIR
                     LQPRSWADWEPVRGGLAAHALLERRYPDAECFVAETGPYPARLALDPKERPWWRRWTA
                     PAVHHGPVPPGAVQMLWANMALHMSAQPGSLIEQWHRALAVGGFLMFSCLGPDTLREL
                     RGVYRALGWPPPAHDFTDMHDWGDMLVHAGFAEPVMDMERITLTWETPQRLLEELREL
                     GANLHPARYPALRGAGWKQRL"
BASE COUNT         2172 a         3953 c         3976 g         1874 t
ORIGIN      
        1 ggcgcgcgtc ctggcgagtt catcctcgag gccggaggga gcgccgccgg tttccgggag
       61 aacttgggac acttcggtcg gggcgcggtc catggcggcc tgttggttat attggtgcgg
      121 agccccataa gaccattgcc ggcctggccg ggcttaggca aattgcctag cggccctagg
      181 gtatttgccg cgacggggtg agagactcag ttgtcccagc cgggcggccg ctcctggcca
      241 tcgcgccggc gctgctcgcg ctcggctcgc tcctgcgcca tttgctcctc cagttgccgg
      301 cgcccctgct tgatcacggc gatgaccttg gcccggtcct tgtgatgcgg gtacatctgt
      361 tcgaacagct gcaggttgtg gcgccggaag cgcatggcgt gtgcccttgc ctcgtgcggt
      421 gcgaagccca ggacctccag aaccgtgcgc gcgctgcgca ggctggcttc gaacacttcg
      481 cgctccaccc gcgcgacgcc gcgatcgcgc agctcgttcc agtgcgtgac gtcgcgggcg
      541 cgggccacca gttcgagatg gggaaaatgc tcgcgcgcca cgtcgacgat cgccagcgat
      601 tgcccggtat cgtcgaccgc gaccaccagc accttggccg ttgctgcgcc cgcggtgcgc
      661 agcaggtcga gccgggtggc atcgccgtag aacaccttgt agccgaaggc ccgcgccgcc
      721 tcgatcatgt cggcatcgtg atccagcacg gtggccgcga taccttgggc cgccagcatc
      781 cgcccgacga tctgcccata gcgcccgaag cccgcgatga tgatgggcgc gtgctgcggt
      841 tcactgattt cctccagccg cggcgtattg cagttggcgt accgcggcag caccagcttg
      901 tcgatggcga ccagcagcaa gggcgtcagc agcatggaca ccgccaccgc cccgatcagc
      961 agcgacgaga tctccgcggg aatcacccgc gcgccggccg cggcctggaa cacgacgaag
     1021 gcgaactcgc ctccttgtgc cagcagcagc gtgaacaccg ggcgttcctg caccggcagg
     1081 ccgatgcccc tggcgagcgc atggatgagc gcggccttga gtgccaggaa gcccaccacg
     1141 atcagcgcca tgagcccggg cgactcgagc agcaccgcga aatcgatgct catgcccacg
     1201 gcgatgaaga acaggcccag cagcaggccc ttgaacggct cgatgtcggt ttccagctcg
     1261 cgccggtact cgctctcggc cagcagcacg cccgccagga acgcacccag cgccatcgac
     1321 aaccccgcca gctgcatcaa agcggcgacg gcgaccacca gcagcagcga ggcggctgtg
     1381 aagatctccg gcgtcctgct cttggcgatc cagcgcagca acgggcgcag cagcaggcgc
     1441 ccgcccagga tgacaccgcc gatcaccgcg aggatcttgg ccccttccag ccagcggctt
     1501 gcagcgctgg tttcggccgc cattccgccc agcaggggca gcagggcgat gatcggaatc
     1561 gccgcgacgt cctggaacag caggatggat aaacccgctt gcccgctgga ggtcggcaac
     1621 aaattgcgct cgcccatgac ctgcagcgaa atcgccgtgg aggacagggc caggcccagc
     1681 gccgccacca ggctcacgcg ccagccgagg cccgccgcca tgccggccgc ccacagcagg
     1741 gcggcacacc ccagcacttg ggcgctgccg gagccgaaga tcggtcggcg caggctccac
     1801 aggcgccgcg gctcgagctc caggcccacc agaaacagca tcaggacgac gccgaactcg
     1861 gcgaagtgaa ggatgtcctc aacgttgctg accaggccca gcccccaggg gccgatggcg
     1921 atgccggcgg ccaggtagcc gatgatcgaa cccagtccca gggcccggga caggggcacg
     1981 gcgatgacag ccgcgcccag gtagatcagg ctgttggtga gccaggcggg tgcgtgttcc
     2041 atgtttctct ctgtcattcc gggcttgacc cggaatccac ggtggcgcgc cgagatggat
     2101 cccggatcaa gtccgggatg acgtccgttc cggtgcgcgg ccggtcggtc ggcggcacgt
     2161 cgcaggccgg gcattcaccg agttcgtcga gttccggcca ttgcggatag ctgcgcaggc
     2221 gttccccgaa aacggccaca tggtcggcca ccgactgctc gctcgcgcgg tgggcaccgt
     2281 gcagcaggag cggcggcagg aagcgcatgc cgcacagcgc ggcagtctgc tcgtagggcg
     2341 gcaggaaggc gtcgaagaag taccggttgt agctttgcgg gtgataggaa ctttccggcc
     2401 caccggtggt ggcgaccagc cacaggtcct tgccccgcag cgcggtcccg cccggaccat
     2461 aggcccagcc ataagtgagc acttcatcca gccaaagctt gagcagcgcc ggcatcgaat
     2521 accactggat cggatgaagc agcacgatca agctggcgtc ggctgcccgc gcctgctcgg
     2581 cggcgacgtc gatgtcgtag tcggggtagc tgccgtacag gtcctggacc tgcacgccgg
     2641 tggtttcccg cgcggcggcg acgagacggc gattgacacg cgactcacgc caattcggat
     2701 gggccgccag caccaggacg ccgcgagaag gttcttcttt tgcgatctca tccataccgg
     2761 taacatagcg caaggcaggg gcttttgccc aactggccaa gaatgcaata agtagaggag
     2821 cccatcatgc cggtcgtcgt tgttgccaat cccaaaggcg gcgtcggaaa gtccacgctg
     2881 gcgaccaatc tcgcgggctt ccttgccagc aagggccatg ccgtcatgct gggagacgcc
     2941 gatcgccagc agtcctcgcg attgtggctg ggcctgcgcc cgaatggcgc gcggcccatc
     3001 agcacatggc agctcagtga gggcaatatc gtcaagcccc ccaaaggcac gacccatgtg
     3061 gtgctggaca cgccggccgg catgcacgga aaggtcttca aggacgtgct ccagctggcc
     3121 gaccgggtga tcgtgccctt gcagccgagc atcttcgaca tattcgccac ccgggccttc
     3181 ctggatgagc ttgcggagca ccgcaaggcc agcaagctgc aggtggggat cgtcggcatg
     3241 cgggtcgacg cgcggacgat cgcggccgaa aagttgcacg agttcgtcga cgcgctgggt
     3301 ctgcccgtgc tgggctatct gcgccacacc cagaactaca tacacctcgc cgcgcggggg
     3361 ctgacgcttt tcgatgtggc gccgggccgc gtcgagcgcg acctggccca atgggagggc
     3421 atctgtcgct ggacggacag ctgaggcgaa aggaccaggc actggtgaca ggggctaagg
     3481 taaagtcagt gagtcctgca aatagccaca gatcgccagc ccagatgaaa acccttcagc
     3541 acctctccga actgccggca ctcgtgggcc aggaaatcgc cgtcagcgac tggatcacca
     3601 tcacccagga gcaggtgaac ctgtttgccc aagcgacggg cgaccaccag tggatccatg
     3661 tggacgtcga aaaggccaaa gccgggcctt tcggcgcgcc catcgcccac ggcttcctga
     3721 ccttgtcgct gatcccgaaa tttttccaga cgtcgttcga gatcctcgat tcgcgaatgg
     3781 gcgtcaacta cggcttgaac aaggtgcgtt tcaccgcgcc cgtgccagtt ggcagccgtc
     3841 tgcgcgcgca catgaagctg ctcgtctgtg agccgatcga aaacaatgga cagcagatgg
     3901 cgtggctggt gactgtggag cgcgagggca gcgacaagcc cgtgtgcatt gccgaatcgc
     3961 tctcgcggcg ctatccctag ggactcgcgc tgcacgttgt tccgcgcgat ccggaaaggg
     4021 cgggcagttg ggctgcccta gtggccaggc ggcgccggct gcttgatctc cggcgcgaag
     4081 aacgcgccgc gcgtcagcaa cgtgtagtcg gcctccagcg ctccgatccc cagcagcacc
     4141 atcagcaaca agggaggcac caggatggca tagaccagcg cgagccgctc ccacagcatg
     4201 tgcatgaaga ccgagacgat caagcccgcc ttcagcagca tgaacacgat aatcagcgtc
     4261 catcggagca agccttggac gtgcatgtaa tcgaccatgt aagacgcggc gctgagcacg
     4321 aacagcaggg cccagatctt gaagtagatg ccgagcggat gctgctggcc ctgggcgtgc
     4381 gcgccggggt gctccccggc aggcgcggca tgtgggtggt cggtgtgttc catgtgcttg
     4441 cccctcgtgc taccagagat agaagaacgc gaagatgaag acccatacca gatccacgaa
     4501 gtgccagtac aggccgagga tttcgacgat ctcgtagttg cccctgcggc cggtgagaaa
     4561 ccccggccgc tcctggtcga gatccccgcg cagcaccttg accgccacga tgatcaggaa
     4621 gatcactccg atcgtcacgt gggtgccgtg gaagccggtg atcatgaaga acgaggagcc
     4681 gaactgcgcc gctccccagg ggttgcccca gggacggacg ccttcggaaa tcagcttgta
     4741 ccactcgaat gcctgcatgc ccacgaaggt ggcgcccagg gccgccgtcg cgatcagcaa
     4801 catgaaggtg gtcttgcggt cccgccggta gccgaaattc accgccagcg ccatcgtgcc
     4861 gctgctgctg atgaggacga aggtcatgat cgcgatcagc aacaacggaa cattccggcc
     4921 gaacatcatc agcgagaaca cctcgctcgt attgggccat ggcacggtgg tggacgcccg
     4981 caccgtcatg tacgagatca ggaagctgcc gaagatgaag gtgtcgctga gcaggaagat
     5041 ccacatcatc ggcttgcccc atggcacgcc cttgaaggcg cgttggtccg aggacacgtc
     5101 cgcgacgatg ctttgccagg ggccggggcg gggcagcggt gctgccgtac cggttgcgtc
     5161 gtgcgtcgcc atgaggttcg cctccttgat cacaacgggg tggacgagca gatcgccaag
     5221 ccaacccctt ccgacacgag cagcgcatac agcaccaccc agacggcgag caggtaatgc
     5281 cagtaggtcg cgcacagttc gacgccgagc ctgatcctgg ccgggtccgt tccccgccac
     5341 gcccgcgccg atgcgcgggc ccatgccacc aggccgccga gcacatgcag gccgtgcact
     5401 gcggtcaaca ggtagaaaaa ggccgtggca gcgctgctcg agacgaagta ccccgcgtca
     5461 ttgagttgct tccagacgaa cagctgtccg gcgaggaagc cgaaggtcag cacgccgctc
     5521 gcccacaggc cgcgccgcaa gttgtcccgg tccgcgcggc gggcggcatg caccgtccac
     5581 tccatcgcca ggctggcgcc aaccagaagc gcggtgttca gcatcagcag cctgggccgg
     5641 ggcagcgggg tccagtccgc caggcccagg cgcatggcat aggcgctcac gaacagcacg
     5701 aacagcgacg tggcgactgc gaggaacacc cacaaggcca ttttggccgc cggcggggcc
     5761 gccgcgtcgc cgcccacgtc gggaactgcg acctgcgcct gccacggctg cacgttgatc
     5821 gtctgccgga acagccacca cacgatgaca gccatcagga cggagaggaa aaccaggccg
     5881 atggtcatgc ggctaccctc ctgtgggctg cggggtcttt tcccaggctt cggccgggac
     5941 gtcggaaggt gcgacgttct gcgggatgaa gtcggtcttg aggcccggaa cgccgtagtc
     6001 gtaggcccag cgatagacgg ccggcagttc cttgccgaag tttccgtgcc tgggcggggt
     6061 gtgcgccgtt tgccattcga gcgtggtggc gttccagggg ttgttgccgg ccggcctgcc
     6121 cctgaagtag ctggccgcca ggttgtacag gaacacgatc tgtgcggcgc cgaccacgaa
     6181 ggcggcaagc gagatccatg cgttcagcaa atgcgccgag tccgggatga agttcgtgcc
     6241 cgtgatcgcg tagtagcggc gcgggatgcc catgaaaccc aggtagtgca tcgggtagaa
     6301 gatcgcgtag gtcccgatga aagtgatcca gaagtggatc ttgcccagcg tgtcgtccag
     6361 catgcgcccg gtgatcttcg ggtaccagtg gtagatggcc ccgaataccg tcagcaccgg
     6421 cgcgatcccc atcaccatat ggaaatgcgc cacgacgaac atcgtgtccg acagcggcat
     6481 atccaccacc acgttgccca ggaacaggcc cgtgaggccg ccgttgacga aagtgaagat
     6541 gaacgcgatg gcgaacagca tcggaaccgt gaggtggatg ttgccgcgcc acagcgtcag
     6601 cacccagttg tagaccttga tggcggtggg aaccgcgatg atcagcgtgg tcgtggcgaa
     6661 gaagaagccg aagtacggat tcatgccgct cacgaacatg tggtgtgccc acacgatgaa
     6721 gcttaagccc ccgatcacca ggatggccca gaccatcatg cggtagccga agatgttctt
     6781 cctcgcgtgg gtgctgatca ggtcggacac gatcccgaag gcgggcagcg ccacgatgta
     6841 gacctcgggg tgtccgaaga accagaacag gtgctggaac agcagcggat tgccgcccga
     6901 gtagctcagt tgctggccct tcgagacgag tgcgggcatg aagaagctgg tgcccagcgt
     6961 caagtccagc gtcatcatga tggcgctcac gaacagggcg gggaacgcca gcaaggccag
     7021 gatggtggcc atgaagatgc cccacacggt gagcggcagg cgcatcatcg tcatgccgcg
     7081 cgtgcgggcc tgcagaaccg tgaccacgta attgaggccg cccatggtga acccgatgac
     7141 gaaaaccccc agcgagacaa gcagcaggat gatgccccat tgcgctcccg gcgtgccgtc
     7201 caggatggcc tgcggcgggt acagggtcca gccggcgccg ctcgctcctc cgggcacgaa
     7261 gaagctcgcg acgagcagca gcaccgccag caggtacagc cagtagctga ccatgttcac
     7321 gtaggggaac accatgtcgc gggccccgac catcagcggg atcaggtagt tgccgaaccc
     7381 gccgaggaac agcgccgtga gcaggtagat gaccatgatc atcccgtgca tgctgaccag
     7441 ctggaggtag gtgctcgggt cgatgaagcc gaccgccccc ggaaagccga gctgcaggcg
     7501 catcagccac gaggccacca gcgcgaccaa tccgatcgcg atcgcggtga ccgcgtactg
     7561 gatcgcgatc accttcgcat cctggctcca gatgtacttg ccgatgaacg ttttcgggtg
     7621 gtagagctcg gcctcgccga cctccgcagg cggtgcgaat tgtgattcct cgtgcgcaac
     7681 gtaagtagtc atcgcccctc cgcgcgaatc aacgcgagtt gatgtaggcc aggatgtcgc
     7741 cgatcgccgc gtcgtcggac agcatcccgg cgatcagcgc catctggccg ccgtaaatgt
     7801 cctgcgcgtg ggcgccgcgg acgccgtccc tgaaattctt cagctgcgtg gccatgtacc
     7861 agtcgctcat gccctggagc ctgggtgcgt tggtcgccgc gatcccccgc ccgtcggcgc
     7921 cgtggcaggc ggcgcacgtc gcgtagcgct gccggccctt gtccacatta cccttgatcg
     7981 tcgtcgtcac cggcgcgtcc ggcaggctcg cgatgtaggc cgcgacattg gcgatcgccg
     8041 aatcgtccgc gagcgtggcc gccatcggcg ccatcatctt cccgaacacg tccttgtcgt
     8101 gcgtgccgcg ggctccctgc ttgaacagcc tcagttgccg ttccaggtac caggcgccct
     8161 gtccgctcag tcttggcgca ttcagcgcga cgttgccctc gccgttggcg ccatggcagg
     8221 cagcacaggc cgcataggag gctttgccgg tcacggcgtt gccggcaggc cgggtttgcg
     8281 cctgcgcaaa cgtcgactgg cggtccagcc aggcctggaa ggcgccgggc tcgtccacca
     8341 cgatcctgcc acgcatcgcg aagtgcccga tgccgcacag ttcctcgcaa agcaggtcga
     8401 acgatcctgt ccgcgtgggg gtgaaccaga tatatgtgac cagccccggc accatgtcca
     8461 tcttgacgcg gagttgggcc accgagaagt tgtgcagcac gtccttggag cgaagcagca
     8521 gcttgaccgg ctggcccagc ggtacatgca gctcgggact cgccagcagc acgtcgtcgt
     8581 gcccattggg atcggccgga ttcatcccga aggggttggc gtcgttcacg aagcgagagt
     8641 gcacggtccc cagcttgcca tccttgcccg gcagccgata actccaatgc cactgctggg
     8701 ccatgacttc cgcgaccctg gcgtccttcg gcacgtggat gacgtccgcc cagaccagaa
     8761 ggcccggggc caacatggcg gccacgccga cgccggtgag ggccaggagc caccactcga
     8821 gcttcttgtt ctcgggctca taggctgcgc gtccgccttc gcgatggcgg tagcggatca
     8881 ccgccatcgc catgaacaag ctgaccgcca cgaacacgac ccctgtcacc cagaaggtga
     8941 tggagatcgt gttgtcgatg gtgccccagt tggaggcaat cggcgtgaag taccaggggc
     9001 tcaggaagtg gaacaggacc gagccgaggg ccagcaggat caatgcgatg gcgatcggca
     9061 tggctagcgt gcggtcctgt cgttgatgac gagaaggatg agcgcaattg cgatggtcat
     9121 gggcgttctt gtgctctgat gccggtttca ggggaagacc ggagagaggc gatcgacgcc
     9181 ttcgaaatgg gcctccggat gggcggcccg cagcagcgac gcgatggctt gcgcctgccg
     9241 gtacggcaca tcgaccatga gcagaatccg cccctgggcg atggcgctct cgaagcgcac
     9301 gagccgcggg ctcgggatgg agatgccgat catgctcgac gaccaggcgc cgaagaggcc
     9361 gccgaagacc gccaggatgc cggccacctc ccactgcggc gcatcgccga cgattgggaa
     9421 gaacagcgcg gccagcaagc ccgtgcaaat gccggtggcg ccgccgaata ccaggccccg
     9481 ttgcgccgca tggatcacgt cggaggtctg cagcacattg gcggcgtgca ggccggtgag
     9541 gtccaggccg tcgcgggccg cgaaatgcat gtgccgcgat tcgatgcccg ctccccgcag
     9601 gccggccatg gtccttccgg cgctcgccag atcgggcaac aaccaataaa tgcgttgctt
     9661 catggaagca tcatcctttc gtcacgggtg gtgacacggg gacagaccag tggggactta
     9721 tacgcctgcg aacaagcgcg cgcaatcgga caaattcgcc agcgcgccgt cggcctttcc
     9781 aatggatggg cttggcgagg ccgtctcgtt ttttgcgctt gcggccggcc gattggattt
     9841 cgacctcacg gctgagttgg cctgccagcc cggcctggca cggatgcacg gagccggcgg
     9901 cgccccatgc gccgcgcgaa aaaaacacct acgacaacgg cgctggcgac tcgcgcgggc
     9961 cgaagccgtt ttgccgccag gcctcgtaca cggtgacagc gacggcattg gaaagattga
    10021 ggctgcgctg gcccggtcgc atgggcagtt tcaagcgctg ccctggcggc agcgaatcac
    10081 gcagggccgg cgccaggccg cgtgtttcgg cgccgaacac cagccagtcg ccgggccgga
    10141 acgcggtctc gtgcaccagc cgcgcgcccc gtgtcgtcag cgcgaacagg cgcagcggat
    10201 cgggttgctg tgtgtccaga aagcgctgcc agccggcatg gcaccggacg tcggcgtatt
    10261 cgtggtaatc cagaccggcg cgcctcaggt gcttgtcgtc catggaaaag cccaggggct
    10321 cgatcaggtg cagcgtgcag ccggtgttgg cggccagccg gatcacgttg ccggtattgg
    10381 gcgggatctc cggctcgacc aggacgatgt ggaacatttg atgacgaatg acgaatgcga
    10441 cgcggtgccg tcatcggtca ctggttcgcg ccaggaccac cgccgtgatc cgcgcggctc
    10501 ccgcttgtcg caatgtctgc gccgcggcgt gaagcgaggc cccgctggtc atgacgtcgt
    10561 ccaccagcac cacgctgcgc ccgcgcacgc tcgttgccgg caacggatcg accgcgaaca
    10621 ccccctcgac attgcccagc ctgtccgcgc ggtccagccc cgcctgcgcg gccgtgtgcc
    10681 gcacccgaag cagcaggccg ggcaccgtct tgcgtggcgc gagcgctcgt gccagcaaca
    10741 acgcctgatt gaagccgcgc tcggccagcc gtgcgcgcga caacggcatc ggcagcacca
    10801 ggtccgcttc gtccagcgcc ggctcgaccc agggcgcgct gcgcatcaag gtcgcgaagc
    10861 tgcgtgccca ccccgcctgc ccgtggaact tgtactgcgc gatcagcgcc ggccagggga
    10921 actcatagga aaccgcggca tggcatccgt ccagcggcgg tggcgcgccc acgcagtggc
    10981 cgcattcggc aacgccggcc ggaaccggca gcgcacagcg gcggcatcgt gcctggggct
    11041 gcgcaaaccg cgcgacgcaa gcctcgcaca ccggctgggc cggccaggcg cggcaaaccg
    11101 cgcattggcc gggcagcgcg gccgcaagcc ggtcggtcag gcggcgcagc acggcatgct
    11161 ccggggaggc tgattcatcc gtttcctgga acgcgagcgc gcatggcgag agtgggtcgg
    11221 atcttgccgg ctcaatatac tcgctcaacc gagtgccatg accgacgaac gcccgcctac
    11281 gatcgacccc gttgccgctg cccgttgggg gcgggtcgcg ccggcgcgct caccctggct
    11341 gcatgaggaa gtggcccgac gcatggaaga ccggctgcaa tggattcggc tgcaaccccg
    11401 gtcctgggcc gactgggaac cggtgcgcgg cggcctggcc gcccatgcgc tgctggagcg
    11461 ccgctatccc gatgccgagt gcttcgtcgc cgaaaccggg ccgtatcctg cgcgtctggc
    11521 gctggacccc aaggagcggc cctggtggcg gcgctggacc gcgccggccg tgcaccacgg
    11581 ccctgtcccg ccgggggccg tccagatgct gtgggcgaac atggcactgc acatgtccgc
    11641 gcagcccggc agcctgatcg agcaatggca ccgggcgctt gccgtcggcg gcttcctgat
    11701 gttctcctgc ctggggcccg acaccttgcg ggaattgcgc ggcgtctacc gcgcgttggg
    11761 ctggccgccg ccggcgcatg acttcaccga catgcacgat tggggcgaca tgctggttca
    11821 cgccggcttc gccgagccgg tgatggacat ggagcgcatc acgctgacct gggaaacgcc
    11881 gcagcgcctg ctcgaagagt tgagggagct gggtgccaat ctccaccccg cccggtaccc
    11941 ggccctgcgc ggcgccggct ggaagcagcg gctgc
//
LOCUS       DASWIY010000424         2821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1402159, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000424 DASWIY010000000
VERSION     DASWIY010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402159"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..927)
                     /locus_tag="VGA59_15130"
     CDS             complement(31..927)
                     /locus_tag="VGA59_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9721050.1"
                     /translation="MQKLSRRQLILLVLLTIVWGINWPVMKLGVTGFPALTFRAISIW
                     LGVPVLALGLVLLKVPFRLPRRHWPELLWLAATNMFVWHACIILAVKTLSSGRAAILG
                     YTMPIFSAVIGAWLFSAALSRRGWLGVSAAALGVALLLWHELTGLAGQPVGVALALTA
                     AATWALGTQLLRRTRMDVPTLTLSFWMTVLTAAVMTALSVLFEKSDWQAPSSTVWAAL
                     LFNAVLIFGFAHATWFYLARALPPVASTLSVMFIPVVGVFSGALWLGEVLHWQDWAAV
                     VLMVVAIASVLWPPAVRAPQAA"
     gene            965..1807
                     /gene="pyrF"
                     /locus_tag="VGA59_15135"
     CDS             965..1807
                     /gene="pyrF"
                     /locus_tag="VGA59_15135"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX9721051.1"
                     /translation="MTFLEMLRNAQARNASMLCVGLDPEPTRFPSDLKGDAGRIYDFC
                     ARIVDATADLVIAFKPQIAYFAAHRAEVQLERLMEHMRRAAPQVPVILDAKRGDIGST
                     AEQYAKEAFERYGADAVTLSPFMGFDSVQPYLKYRGKGAFLLCRTSNPGGDDFQNQRL
                     SSVEGQPLLYEHVARLAQGPWNVNGQLGLVVGATYPAEIGRVRQVAPTVPLLIPGVGA
                     QGGDAVATVKAGWRADGPIIVNSSRAILYASDGEGYAEAARREARRTRDVLEAAKQQA
                     VGHD"
     gene            1995..2207
                     /locus_tag="VGA59_15140"
     CDS             1995..2207
                     /locus_tag="VGA59_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721052.1"
                     /translation="MIDSATTQFELRFLSLSDSERGYAFPCDPKGQVDLNQLSERTRN
                     NYFYARAMVGRELAVPAVRPAAKVLH"
     gene            complement(2336..2701)
                     /locus_tag="VGA59_15145"
     CDS             complement(2336..2701)
                     /locus_tag="VGA59_15145"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9721053.1"
                     /translation="MANPFVHVELSSTDVGAAKTFYGKLFDWKLEDMPMGPDMTYTMI
                     NVGEGTGGGMMKNMIPGSPSFWLAYVQVDDIEKATRKAESLGAKVMKDVTEVMGAGWL
                     SVIVDPTGAALGLWKPKAK"
BASE COUNT          528 a          936 c          912 g          445 t
ORIGIN      
        1 aattgatgac ggaagggcct cgcactcgca tcaggccgcc tggggcgcgc gaacggccgg
       61 gggccacagg accgaggcga tcgccaccac catcagcaca accgctgccc agtcctgcca
      121 gtgcagcact tcgcccagcc ataaggcgcc gctgaaaacg ccgacaacgg gaatgaacat
      181 cacactcaag gtcgaagcca ccggcggcaa cgcgcgagcc aggtagaacc aggtggcatg
      241 ggcaaagccg aagatcagca cggcattgaa caggagggcc gcccacacgg tactgctggg
      301 cgcctgccag tcggacttct cgaacagcac cgacagcgcc gtcatgacgg ctgcggtcag
      361 caccgtcatc cagaacgata gagtgagcgt cggcacgtcc atgcgggtgc gcctgagcaa
      421 ctgcgtgccc agcgcccagg tggccgccgc cgtcagtgcc agcgccacgc ccaccggctg
      481 gccggccagg ccggtgagct cgtgccacag cagcaatgcc acgccgagcg ccgcggcgct
      541 gacgcccagc cagccacgcc gggacaatgc ggcggagaac agccaggcgc cgatcactgc
      601 cgagaaaatc ggcatcgtgt aacccagtat ggccgcgcga ccgctggaca gcgtcttgac
      661 ggccaggatg atgcaggcgt gccagacgaa catgttggtg gccgccagcc acagcagctc
      721 gggccagtgg cggcgcggca ggcggaacgg caccttcagc aacaccaggc cgagggccaa
      781 caccggcacg cccagccaga tcgagatcgc gcggaatgtg agggccggaa acccggtcac
      841 acccagcttc atcaccggcc agttgatccc ccacacgatg gtgagcagga ccaggaggat
      901 gagttgtcgc cggctgagct tttgcatggg ttcgcattgt ggctcgatcc ttacaatcgc
      961 gcgcatgacc ttcctcgaga tgctgcgcaa tgcgcaggca cgcaacgctt ccatgctgtg
     1021 cgtgggactg gaccccgagc ccacgcgatt tccctcggac ctgaagggcg acgccggcag
     1081 gatctacgac ttctgcgcgc gcatcgtcga tgccacggcc gacctggtca tcgcgttcaa
     1141 gccgcagatc gcctactttg ccgcgcaccg cgccgaggtg cagctcgagc ggctgatgga
     1201 acatatgcgc agggcggccc cgcaagtgcc ggtgattctc gatgccaagc gcggcgacat
     1261 cggatcgacc gccgagcagt acgcgaagga ggcgttcgag cgctacggtg ccgatgcggt
     1321 gacgctgtcg ccgttcatgg gcttcgactc ggtgcagccc tatttgaagt accggggcaa
     1381 gggagccttc ctgctgtgcc gcacgtccaa ccccgggggc gatgactttc agaaccagcg
     1441 cctgtcctcg gtggaagggc aacccctgct gtacgagcat gtcgcgcgcc tcgcgcaggg
     1501 gccgtggaac gtcaacgggc aattgggcct ggtggtgggc gcgacctacc ccgccgagat
     1561 cgggcgcgtg cgccaggtgg cgccgaccgt gccgctgctg atcccgggag tgggcgcgca
     1621 gggcggcgat gcggtcgcca ccgtcaaggc cggctggcgt gccgacggcc cgatcatcgt
     1681 gaattcctcg cgggcgattc tctatgcgtc ggatggggag ggctacgccg aggcggcaag
     1741 gcgagaggca cgccggacca gggatgtgct cgaggcggcg aagcagcagg ccgtcggtca
     1801 cgactgactt gagtgacggc tccggctccc acaggtcgga cgattcctag ccctcctcgg
     1861 caggattgaa ccgggcaaat cggacggcgc cgagcacccg attcagaggg cgccgatgtc
     1921 gaaggcaggg gtgtgcagcc atagtagatg cacgccccag ggcatccaac gcaacgcctt
     1981 cccggagaca cgcaatgatc gattccgcca cgacccaatt cgaactgcgc ttcctttcct
     2041 tgtccgacag cgagcgtggc tacgcattcc cgtgcgaccc gaaggggcag gtcgacctga
     2101 accagctgag cgaacgtacg cgcaacaact atttctacgc ccgcgccatg gtgggacgcg
     2161 agctggccgt ccctgcggtg cgccccgcgg ccaaggtgct gcactgagca ggctctgcta
     2221 acgcaccttc ggcgcgcgtt cattattcga acgcgattcg agccaccttc aaagcagccg
     2281 ctgccttggc gggcgggcac ctgggcccgc cagggcgact atccggcgct tgcgctcact
     2341 tcgccttcgg cttccacagc cccagcgcgg ccccggtggg atcgacgatg acgctcagcc
     2401 agccggcgcc catcacttcg gtcacgtcct tcatcacttt ggcgcccagc gattccgcct
     2461 tgcgcgtggc cttctcgatg tcgtccacct gcacataggc cagccagaac gagggcgaac
     2521 cgggaatcat gttcttcatc atgccgccgc cagtgccttc gccgacgttg atcatggtgt
     2581 acgtcatgtc cgggcccatc ggcatgtctt cgagcttcca gtcgaacagc ttgccgtaga
     2641 aggtcttggc ggcgccgacg tccgtgctgc tcagctcgac gtgcacaaag ggatttgcca
     2701 tttgatctct ccagttgagg ttgaagaaag cagccgctgg attgcgactg atctgacaat
     2761 gacggcccaa tttgacgctt ttcgacagga gcggtgcgtc agctttgtaa agatggccgc
     2821 a
//
LOCUS       DASWIY010000425         5758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3600462, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000425 DASWIY010000000
VERSION     DASWIY010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3600462"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VGA59_15150"
     CDS             <1..1360
                     /locus_tag="VGA59_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX9721054.1"
                     /translation="GRLDAAWARWSRPWTTTAWIFLTLGIGLGSWWAYYELGWGGWWF
                     WDPVENASFMPWLIGTALMHSLAVTEKRGSFKSWTVLLAIGAFSLSLLGTFLVRSGVL
                     TSVHAFATDPKRGIFILVLLVLVIGSSLLLFAWRAPKVALGGSFGLLSRESLLLVGNV
                     LLVVAAGSVLLGTLYPLLVDALGLGKISVGPPYFNSVFVPVMAPVLFLMAVVPLARWK
                     NAVLKDMLARLWVPASVAVVSAIVIPWLLGGWTPLTALGILLAAWIATSMTMQIAKRL
                     KAGNPPLSFWGMHLGHLGIGVFVVGVTLVGGFQEEKDVRMEPGDTTTVGGYQFKFMGV
                     RDVQGPNYVASRGEFQVSKDGRPGRTLFPEKRNYQSSSMPMTEAAIDPGVTRDVYVSL
                     GEPLEGQAWAVRVYYKPFVNWIWGGCLLMALGGLFAMADRRYRLVVKSRAEAAGLRRA
                     TA"
     gene            1357..1890
                     /locus_tag="VGA59_15155"
     CDS             1357..1890
                     /locus_tag="VGA59_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX9721055.1"
                     /translation="MKARFLIPLALFGVLVGFLAVGLQRDPREIPSPLVAKPAPAFKT
                     QQLVMADQTFSPADMKGKVWMLNVWASWCVACKVEHPLLVEMQRSNVVPLIGLDYKDK
                     RPDALKFLAQNGDPYNLSAMDGDGRIGIDYGVYGVPETFLIDRNGVIRHKQIGPITPD
                     ALQKTILPLIEKLKTES"
     gene            1887..2345
                     /locus_tag="VGA59_15160"
     CDS             1887..2345
                     /locus_tag="VGA59_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX9721056.1"
                     /translation="MKRLFALLLLALVLGAPLAKEAAPLAEDPAVEQRLVAISEEMRC
                     LVCQNESLSASRSDLAQDLRRELRTLIKQGKTDAEIREFMVSRYGDFVLYRPRVSPTT
                     WLLWGGPFVLMIVGVVLLLTYLRRRNRTVGEAHLSEDETRRAEALLKEDK"
     gene            2342..3568
                     /gene="ccmI"
                     /locus_tag="VGA59_15165"
     CDS             2342..3568
                     /gene="ccmI"
                     /locus_tag="VGA59_15165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX9721057.1"
                     /translation="MTGFVIAAAVIVALVLVLLMRPFFRRAGPRTTSHQDLNSAIYRE
                     QLAKLEQDLAEGTLGKDDYAQARAELQRRLLDESQAEVAAVTMNPPRKTMIGIALAVP
                     LVAGAFYLLIGSPASMSGDGGAAQVAGNQELEQMVASLAAKLEKEPDNPKGWAMLARS
                     YKVMGKTVEAEKAFERAGSFIDDDASMLAAYADTIASNNGGQLAGKPAMLIQKALKAD
                     PDHPMSLWLSGTADLETRNYSQALRTFERLAALLPPGSDDARMLEGAINDVRARAGLA
                     AKASPAVPAAPAVTMSAAAGGNVSGTVELDPSLKGKAGPDDTVMVLARLPGSRMPLAA
                     VRLRASQLPVKFVLDDSLSMNPQSPISAATVVEVEARISKSGLAKPEPGDLISAPQTV
                     KVGASGVALRVAQVRP"
     gene            complement(3603..4847)
                     /locus_tag="VGA59_15170"
     CDS             complement(3603..4847)
                     /locus_tag="VGA59_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9721058.1"
                     /translation="MKRHLASLALAAMLTSGAVQAQQATFKIAYIDPLSGPFANIGQL
                     MLTNIQYAVQDINAKGGVLKHSQLQLLQFDSKLSAQESQSALQAAIDQGARAVVTGGS
                     GSSVVAALVSAAARHNERNPDRAIIVLNHSSIDPELTGKACSFWHFQFEANTAMKMKA
                     IANYIKKQPSVKNVYLFNQDYAHGKQWARYGQELVGLARPDVKFVGSDLHPIGRVKDF
                     SPYISKIKATGADSVITGNWGQDLTLLLRAAGDAGYDLRYFNHSAGGSPGLVVAVSQS
                     KIGQLTWVAEWHPGQQDVPKADALFKAYKAKTGQEYLAPRMHLAVHMLAAAINKAQSA
                     EPLKVARAFEDLTFESVMGPVRMRAEDHQLLMPQVVNTIAPVDGKNVRVGWEGTNYGF
                     RTDAVYTGNELAQGTECRMQRP"
     gene            complement(4844..5548)
                     /locus_tag="VGA59_15175"
     CDS             complement(4844..5548)
                     /locus_tag="VGA59_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9721059.1"
                     /translation="MRQDTTDRAGALPPAPVSGRQKSATPGTDAKRERILQAAEWLFH
                     EQGYADTTMDQIVGKLGVTKPYVYYYFHNKQELFELLAWEPSVACFTVLDGPDDGRPA
                     HEKVARALRDIIRLTIDYHPSSFFAFREPQAFRPEFAAELRRLANHFYTKLCALLEQA
                     RADGTLEFGDTRVTAQAACSISGYMYTWYRPEGRLSPEEMVRELTQLAWRVIGLHTTA
                     PGRRPTNLHLIPKEPP"
BASE COUNT          894 a         1863 c         1963 g         1038 t
ORIGIN      
        1 cggccggctc gacgcggcct gggcccgctg gtcgcggccc tggacgacga cggcctggat
       61 tttcctgacc ctcgggatcg gcctgggctc ctggtgggcg tattacgagc tgggctgggg
      121 cggctggtgg ttctgggacc cggtggagaa cgcctccttc atgccctggc tgatcggcac
      181 cgccttgatg cactcgctgg ccgtgaccga aaagcgcggc agcttcaaga gctggaccgt
      241 gctgctggcg atcggcgcat tctcgctctc gctgctggga accttcctgg tgcgttccgg
      301 cgtgctgact tcggtccatg cgttcgccac cgaccccaag cgcggcatct tcatcctggt
      361 gctgctggtg ctggtgatcg gctcgtcgct gctgctcttc gcctggcgcg cgcccaaggt
      421 cgcactgggc gggtcgttcg gattgctgtc gcgcgaatcg ctgctgctgg tgggaaatgt
      481 gctgctggtg gtggccgccg gctcggttct gctgggcacg ctgtatccgc tgctggtgga
      541 cgcgctcggg ctcggcaaga tctccgtggg gccgccttat ttcaattcgg tgttcgtgcc
      601 cgtcatggcg cctgtactgt tcctgatggc cgtggttccg ctggcgcgat ggaagaacgc
      661 cgttctcaag gacatgctcg cgcggctgtg ggttcccgcg tcggtggccg tggtatcggc
      721 catcgtcatt ccctggctgc tgggcggatg gacgccgctc accgcgctgg gcatcctgct
      781 ggcggcctgg atcgccacgt cgatgaccat gcagatcgcc aagcggctga aggcgggcaa
      841 tcccccgctc tcgttctggg gcatgcacct gggccacctg gggatcggcg tgttcgtggt
      901 cggcgtcacc ctggtcggcg gcttccagga agaaaaagac gtgcgcatgg agccgggcga
      961 taccacgacc gtcgggggct accagttcaa gttcatgggt gtcagggacg tccagggccc
     1021 gaactacgtg gcttcgcgcg gagaattcca ggtgagcaag gacggccggc cgggccgcac
     1081 cctgttcccc gaaaagcgca actaccagtc ctcgtccatg ccgatgaccg aggccgctat
     1141 cgaccccggc gtgacccgcg acgtgtatgt gtccctcggt gagccgctcg agggccaggc
     1201 ttgggccgtg cgcgtgtact acaagccctt tgtcaactgg atctggggcg gctgcctgct
     1261 gatggccctg ggtggcctgt tcgcgatggc cgaccggcgt taccggctgg tcgtgaagtc
     1321 ccgggccgag gcggcagggc tgcggagggc cacggcatga aggcaagatt cctcatcccc
     1381 ctggcgctgt tcggcgtgct ggtcggcttt ctggccgtgg gcctgcagcg cgacccgcgc
     1441 gagattccgt ccccgctggt cgccaagccg gctcccgcgt tcaagacgca gcaactcgtg
     1501 atggccgacc agaccttctc gccggccgac atgaaaggca aggtctggat gctcaacgtg
     1561 tgggcttcct ggtgcgtggc atgcaaggtg gagcacccgc tgctggtgga aatgcagcgc
     1621 tccaacgtgg tgccgctgat cgggctggat tacaaggaca agcgtcccga tgcgctcaag
     1681 ttcctggcac agaacggaga cccctacaac ctgtcggcga tggacgggga tgggcgtatc
     1741 ggcatcgact acggcgttta cggtgtgccg gaaacgttcc tcatcgacag gaacggcgtc
     1801 atccgccaca agcagatcgg cccgatcacg cccgacgcgc tccagaagac catcctgccg
     1861 ctgatcgaga agctgaaaac ggaatcatga aacgcctgtt cgcattgttg ttgctggcgc
     1921 tcgtgctggg cgcgccgctc gccaaggagg cggcgccgct ggcggaggac ccggccgtgg
     1981 agcaacggct ggtcgccatc tcggaggaga tgcgctgcct ggtgtgccag aacgagtcgc
     2041 tgtcggcgtc gcgctcggac ctcgcgcagg acctgcgccg cgaactgcgc acgttgatca
     2101 agcagggcaa gaccgacgcg gagatccgcg agttcatggt cagccgctat ggcgacttcg
     2161 ttctttaccg tccgcgcgtg agccccacga cctggctgct gtggggggga ccgttcgtgc
     2221 tgatgatcgt cggcgtcgtg ctgctactca cctacttgcg ccgccgcaac cgcactgtcg
     2281 gtgaagccca cctgtccgaa gatgaaaccc gccgcgccga ggcgctgctg aaggaagaca
     2341 aatgaccgga ttcgtgatcg ccgccgcggt gatcgtcgcg ctcgtgctgg tgctgctgat
     2401 gcggccgttc tttcgcaggg ccggcccccg caccacctcc caccaggacc tgaactcggc
     2461 gatctaccgc gagcagctgg ccaagctgga acaggatctt gcggagggca cgctcggcaa
     2521 ggacgattac gcccaggccc gtgccgagct gcagcgccgc ctgctggacg aaagccaggc
     2581 cgaagtggcc gccgtgacca tgaatccgcc gcgcaagacg atgatcggca tcgcgctggc
     2641 ggtcccgctg gtcgccggcg ctttctacct gctgatcgga agccccgcga gcatgtccgg
     2701 ggacggcggt gccgcccaag tggccggcaa ccaggagctc gagcaaatgg ttgcctcact
     2761 ggcggcgaag ctggagaagg aacccgacaa ccccaaggga tgggccatgc tcgcgcgctc
     2821 ctacaaggtg atgggcaaga ctgtcgaggc cgaaaaggcc ttcgagcggg ccggctcgtt
     2881 catcgacgac gatgcgtcga tgctcgccgc ctacgcggac acgatcgcga gcaacaacgg
     2941 cgggcagctc gccggcaagc cggccatgct gatccagaaa gcgttgaagg ccgatccgga
     3001 ccacccgatg tcgttgtggc tgtccggtac ggccgatctc gaaaccagga actacagcca
     3061 ggccttgcgg acattcgagc ggctggcggc gctgctgccg cccggctccg acgatgcgcg
     3121 catgctggaa ggcgcgatca acgacgttcg tgccagggcg ggcctggccg ccaaagcgag
     3181 cccggccgtc ccggcggcgc cggcagtgac gatgtcggcg gcagccggcg gcaacgtcag
     3241 cgggaccgtt gaactcgatc catctctgaa gggcaaggcc ggtcccgacg ataccgtgat
     3301 ggtgttggcc cggttgccgg gcagccgcat gccgctggcg gcggtacggc ttcgcgccag
     3361 ccagcttccc gtgaagttcg tgctcgacga ttcgctgtcg atgaatccgc agtcgcccat
     3421 ttccgccgcc acggtagtcg aggtggaagc gcgcatctcg aagtccggcc tggcgaagcc
     3481 cgagcccggc gacctgatct cggcccccca gaccgtcaag gtcggcgcga gcggcgtggc
     3541 cctgcgtgtg gcgcaggtac gtccgtaagg cagaagacgg acactgccct acggcgtcgg
     3601 gtctacgggc gctgcatcct gcactccgtt ccctgagcca gctcattgcc cgtgtacacg
     3661 gcgtcggttc ggaaaccgta gttcgtgccc tcccagccga cccgcacgtt cttgccgtcc
     3721 accggcgcga tggtgttgac gacctgcggc atcagcagct gatgatcctc cgcgcgcatg
     3781 cgcactgggc ccatcaccga ttcgaacgtc aggtcctcga acgccctggc gaccttgagc
     3841 ggctccgccg actgcgcctt gttgatcgcg gcggccagca tgtgcaccgc gaggtgcatg
     3901 cgcggcgcca ggtactcctg gcccgtcttg gccttgtagg ctttgaacag cgcgtcggcc
     3961 ttgggcacgt cctgctggcc cggatgccat tccgccaccc aggtcagctg cccgatcttc
     4021 gattgcgaca cggccaccac caagccgggc gatccgccgg cgctgtggtt gaaatagcgc
     4081 aggtcgtagc cggcgtcgcc cgcggccctg agcagcaggg tgaggtcctg gccccagttg
     4141 ccggtgatca cactgtcggc gccggtggcc ttgatcttgg agatgtaggg cgagaaatcc
     4201 ttgacgcggc cgatcggatg caggtcggag ccgacgaact tcacgtcggg acgggccagg
     4261 cccaccagct cctgcccgta gcgcgcccac tgcttgccat gcgcgtagtc ctggttgaac
     4321 aggtacacgt tcttcacgct cggctgcttc ttgatgtagt tcgcgatcgc tttcatcttc
     4381 atggccgtgt tggcctcgaa ctggaagtgc cagaaactgc aggccttgcc ggtaagttcc
     4441 ggatcgatcg aggaatgatt cagcacgatg atcgcccggt ccggattgcg ctcgttgtgg
     4501 cgcgcggccg cgctcaccag cgccgccacc accgaggagc ccgagccgcc ggtcaccacc
     4561 gcgcgcgctc cctgatcgat tgcggcctgc agggcgctct ggctttcctg tgccgagagc
     4621 ttgctgtcga actgcagcaa ctgcaactgc gagtgcttga gcacgccgcc cttggcgttg
     4681 atgtcctgca cggcgtactg gatgttggtg agcatcagct gaccgatatt cgcgaaaggc
     4741 ccgctcaagg gatcgatgta ggcgatcttg aaggtggcct gctgcgcctg gacagcgccg
     4801 ctggtgagca tggcggctag cgcgagggag gcgagatgtc gtttcatggt ggctccttgg
     4861 ggatgaggtg gaggttggtg ggccggcggc ccggcgccgt cgtgtggagg ccgatcacgc
     4921 gccaagccag ctgcgtgagc tcccggacca tctcctcggg cgagaggcgg ccctcgggcc
     4981 gataccaggt gtacatgtag ccggagatgc tgcaggccgc ctgcgccgtg acgcgggtat
     5041 cgccgaactc gagcgtgccg tcggcgcgcg cctgctccag cagcgcgcac agcttcgtgt
     5101 agaaatgatt ggcaagcctc ctcagttcgg cagcgaactc ggggcggaac gcctgcggtt
     5161 cgcggaaggc gaagaaggac gaggggtggt agtcgatcgt gaggcggatg atgtcccgca
     5221 gcgcccgcgc caccttctcg tgcgccggcc ggccgtcgtc cgggccgtcc agcacggtga
     5281 agcaggccac cgacggctcc cacgccagca gttcgaagag ctcctgcttg ttgtggaagt
     5341 agtagtagac atatggcttg gtgacgccga gcttgccgac gatctggtcc atggtcgtgt
     5401 cggcatagcc ctgctcgtgg aacagccact cggcggcctg caggatgcgt tcccgcttgg
     5461 cgtccgtgcc gggcgtggcc gacttctggc ggcccgacac tggcgccggc gggagggcgc
     5521 ctgcccggtc ggtggtgtct tgtcgcatgc gttctaccat ggggtagcat ttttctaccg
     5581 gccggtatat cgtgcaagcg tttttcccgc ccgactgacc cgggcaaacc ctagaaaggt
     5641 tcgaatgagt gacgccatgg agacaccccg ctacaccgaa aggccgctgc acgagtacct
     5701 gcgccagcac gcgcgcgagc agcccggcaa gcccgcctac ctttggtacg gccaggcc
//
LOCUS       DASWIY010000426         7802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2896141, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000426 DASWIY010000000
VERSION     DASWIY010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2896141"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1172
                     /locus_tag="VGA59_15180"
     CDS             195..1172
                     /locus_tag="VGA59_15180"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9721060.1"
                     /translation="MKTFFHGISRRQVIGAALLAAAPAWGQARYPVKPIRLIVPYPPG
                     GTTDIQARVIAQKMQELAGVTVIVENKAGAGGNIGVDAVLKAPHDGYTIGITAMNSFA
                     INPFLTKRLPYDVTRDVQPITLIGSIPNIVVVNPEVPARTLKELAALGKNRDLTYATP
                     GAGTSVHLAAEMLSDALGLKMRHVPYRGDAPALQDVLGGRVPVMVANLPGVIEYVRAG
                     KLRPLAVTSATRSPLLPDVPTVAESAVPGFDVKAFFALFTARGVPPEIVESLNRDLNK
                     AINHQDVRKRFAELGIDAAGSSVEALTALIYLESKKWGPIVQKTGASWD"
     gene            complement(1240..3609)
                     /gene="rpoD"
                     /locus_tag="VGA59_15185"
     CDS             complement(1240..3609)
                     /gene="rpoD"
                     /locus_tag="VGA59_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX9721061.1"
                     /translation="MPAQKSGKLVKPTAKPAAAKVIKPGVLGKAKLKVVKTAPKPQAK
                     APAMPATKVKPVPLTKSPAKPSEKANAKDEVNKPHKASAAEEVVKKKPGRPPKAQSAE
                     SAAPAKAGAKRGRKPKDAVEGKTVEDLDLSDIEEDLVGEPVAETTEKVKPLRMKISKA
                     KERALMKEFGLDEAILTEEEMLKRRQRLKTLIKLGKTRGYLTHAEITDHLPEKLVDAE
                     TLEVVVSMLNDMGVAVYEQTPDAETLLLTNTGPTAATEEEAEEEAEAALSTVDSEFGR
                     TTDPVRMYMREMGTVELLTREGEIEIAKRIEGGLMAMMEAISASPATIAEILRLANEI
                     REGRVVISTVVDGFSNPNEADDYVAEEDFDEFDADDDDDGNGGSKALTKKLEELKTQA
                     LERFARIQSLFEKVHKIYDKEGYGTPAYVKSQHALSEELMTIRFTAKTIEKLCDMVRG
                     QVDDVRKKERELRRIIVDKCGMPQETFIRDFPPNLLNQKWVEKQAAAGKPWSTIIARN
                     IPPIQELQQKLADLQSRVVVPLAQLKDINKRMNNGEASSREAKKEMIEANLRLVISIA
                     KKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQART
                     IRIPVHMIETINKMNRISRQHLQEFGFEPDAGILAAKMEIPEDKIRKIMKIAKEPISM
                     ETPIGDDDDSHLGDFIEDQANTAPIEAAMQAGLRDVVKDILDSLTPREAKVLRMRFGI
                     EMSTDHTLEEVGKQFDVTRERIRQIEAKALRKLKHPSRSDKLRSFIDTL"
     gene            complement(3859..5697)
                     /gene="dnaG"
                     /locus_tag="VGA59_15190"
     CDS             complement(3859..5697)
                     /gene="dnaG"
                     /locus_tag="VGA59_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX9721062.1"
                     /translation="MAIPQSFIQELLARADVVEIVGRYVQLKKGGANYMGLCPFHGEK
                     SPSFSVSPTKQFYHCFGCGKNGDAIRFLMEHAGMNFVEATKDLAQQYGMQVPEEDASP
                     QDRARAAQQREKQATLTDVLEKACDAYRKHLKESPRAVDYLKGRGVSGGIAKLFGLGY
                     APEGWHSLASVFPNYDDPLLAESGLVIVNEDEDKRYDRFRDRVMFPIRNVKGECIGFG
                     GRVLGEGTPKYLNSPETPVFSKGRELYGLFEAREALREHGYVLVTEGYMDVVALAQLG
                     FPNAVATLGTACTAEHVHKLFRFTDSVVFSFDGDEAGRRAARKALDAALPYATDVRSV
                     KFLFLPAEHDPDSYIREFGREAFSRYVSEATPLSRFLVEASREGCDIGTAEGRARMVA
                     NARPLWSLLPEGALRRQLLGEIAGHVQLEARELGSLWSSGAAGRPGGNLRRSPNSGLT
                     PRSRRLAIRPSIEASASNIARLLLADSALWEVLSSGDHEILCALDGDFGDLFRWIDYQ
                     AHEHGPQPLAALAVGLEGQPFEAVARRIIALDNLQPSGRMVEDPEMPLADFHAAVVKL
                     QLDVLDLELKRVQALPSTDPGRAAEEEQLIRRQFALKKHQTLENRR"
     gene            5836..7482
                     /locus_tag="VGA59_15195"
     CDS             5836..7482
                     /locus_tag="VGA59_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9721063.1"
                     /translation="MPHIFDQDLPRNQANFTPLSPLSFIERTAEVYPQRLAVVHGDLR
                     RNWRELYGRCRQLASALARSGVGKGDTVAVMLPNTPPMVEAHFGIPMAGAVLNAMNTR
                     LDAQTVAFMLDHGEAKVVIVDPEFAGVMKDAIALRQEKRPLLVVDVEDALYTGPVERA
                     GTVGYEAFLAGGDPAFEWKLPADEWDAIALNYTSGTTGNPKGVVYSHRGATANAISNI
                     LEWDMPKHAVYLWTLPMFHCNGWCFPWTVAARAGVNVCLRKVEAKAILDAIKAHGVTH
                     YCGAPIVQGMLVNAPEEMKQGLPTGVKAMVAGAAPPASMIEGMERMGFDLTHVYGLTE
                     VYGPATVCPKHDEWNDVDIGERARLNARQGVRYHLQRDARVIDPQTMKPVPMDGETMG
                     EIMFRGNIMMKGYLKNAAATQEAFAGGWFHTGDLAVQYPDGYMKIKDRSKDIIISGGE
                     NISSIEVEDVLYRHPDVLAAAVVARPDPRWGETPCAFIELKAGATATREDIVAHCKKH
                     LAGFKVPRAVVFGELPKTSTGKIQKFELRKQAGSAEAIDV"
     gene            7569..>7802
                     /locus_tag="VGA59_15200"
     CDS             7569..>7802
                     /locus_tag="VGA59_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX9721064.1"
                     /translation="MPILSHQAAHVVAHPGRFAWSVLKAFKANQGLLLAGAVAYYALL
                     SVVPLLILTVIALSHVIDQAELLQTLGRYLEWLM"
BASE COUNT         1286 a         2487 c         2501 g         1528 t
ORIGIN      
        1 tccaggcgtt tttcgtgctg caccggattg tagtctaatc tgatcgccga atagattaaa
       61 tcgtcgtaaa atgagttgta aacaggaata tagggtttgc actaagccaa tctcccttgc
      121 atttaatcta atcgccgatt agattaaagc ctgtgcggcc gatggccgcg gcggctcatt
      181 tccatggaga caacatgaag accttctttc acggcatttc ccggcggcag gtgatcggcg
      241 cagccctgct cgccgccgca ccagcctggg gccaggcccg ctatcccgtc aagccgatcc
      301 gcctgatcgt cccctatccg ccaggcggca ccaccgacat ccaggcgcgc gtgatcgccc
      361 aaaagatgca ggaactcgcc ggtgtcaccg tcatcgtgga aaacaaggcc ggcgccggcg
      421 gcaacatcgg ggttgatgcg gtgctgaagg cgccacacga cggctacacg atcgggatca
      481 ccgcgatgaa ctccttcgcg atcaacccgt tcctgaccaa gcggctgccc tatgacgtca
      541 cccgggacgt gcagcccatt accctgatcg ggagcattcc gaatatcgtc gtggtcaacc
      601 cggaggtgcc ggcgcgcacg ctcaaggagc tggccgcgct gggtaagaat cgcgacctca
      661 catacgccac gccgggtgcg ggcacctcgg tgcatctggc cgcggaaatg ctttccgacg
      721 ccttgggcct gaagatgcgg cacgttccct atcgcggcga cgcgcccgct ctgcaggacg
      781 tgctgggcgg ccgcgtgccc gtgatggtgg ccaacctgcc gggggtgatc gagtacgtgc
      841 gtgccggcaa gctgcggccc ctggccgtga cgtccgccac gcgttccccg ttgctgccgg
      901 acgtcccgac cgtcgcggaa tcggccgtgc cgggcttcga cgtcaaggct ttctttgcgc
      961 ttttcaccgc ccgcggcgtg ccccccgaaa ttgtcgaatc cctcaatcgc gacctgaaca
     1021 aggcgatcaa ccaccaggac gtgcgcaagc ggttcgccga gcttggcatc gatgccgccg
     1081 gatcgtccgt cgaagcactg acggccctga tctacctgga gtccaagaag tggggcccga
     1141 tcgtccaaaa gactggagcc agctgggact agggggccta tctgcgaatg aaaaaagggg
     1201 cgccgacagg cgccccttct tcgttggcgg cagcgccgct tacaacgtat cgatgaagct
     1261 gcgcagcttg tcgctgcggc tggggtgctt cagtttgcgc agagccttgg cctcgatctg
     1321 gcggatgcgc tcacgggtca cgtcgaactg cttgcccact tcctccagcg tgtggtccgt
     1381 gctcatctcg atgccgaagc gcatgcgcag caccttggct tcgcgcggcg tcagcgagtc
     1441 caggatgtcc ttgaccacgt cgcgcaggcc cgcttgcatc gcggcctcga tcggcgcggt
     1501 gttggcctgg tcctcgatga agtcgcccag atgcgaatcg tcgtcgtcgc cgatcggcgt
     1561 ttccatcgag atcggctcct tggcgatctt catgatcttg cggatcttgt cctcgggaat
     1621 ctccatcttg gccgccagga tgcccgcatc cggctcgaag ccgaactcct gcaggtgctg
     1681 gcgcgagatg cggttcatct tgttgatcgt ttcgatcatg tgcaccggga tgcggatcgt
     1741 gcgcgcctgg tccgcgatcg agcgggtgat ggcctgccgg atccaccagg tggcataggt
     1801 cgagaatttg tagccgcggc ggtattcgaa cttgtccacc gccttcatca ggccgatgtt
     1861 gccttcctgg atcaggtcca ggaactgcag gccgcggttg gtgtacttct tcgcgatcga
     1921 gatcaccagg cgcaggttgg cctcgatcat ttccttcttg gcttcgcgcg aggaagcctc
     1981 gccgttgttc atgcgcttgt tgatgtcctt gagctgcgcc aggggcacga caacccgcga
     2041 ctgcaggtcc gcgagctttt gctgcagttc ctggatcggc gggatgttgc gggcgatgat
     2101 cgtggaccag ggcttgccgg cggcggcctg cttttccacc catttctggt tcagcaggtt
     2161 gggcgggaag tcgcggatga aggtctcctg cggcatgccg cacttgtcca cgatgatgcg
     2221 gcgcagttcg cgctctttct tgcgcacgtc gtcgacctgg ccgcgcacca tgtcgcacag
     2281 cttttcgatt gtcttggccg tgaagcggat ggtcatcagc tcttcggaca aggcatgctg
     2341 ggacttcacg tacgcgggcg tgccgtagcc ttccttgtcg tagatcttgt gcactttttc
     2401 gaacagcgac tggatgcggg cgaagcgttc cagtgcctgg gtcttgagtt cttcgagctt
     2461 cttggtcaac gccttggagc cgccgttgcc gtcgtcgtcg tcgtcggcgt cgaattcgtc
     2521 gaaatcttcc tcggccacgt agtcgtcggc ctcgttcggg ttcgagaagc cgtccaccac
     2581 cgtggagatc acgacccggc cttcgcggat ctcgttggcc agccgcagga tttccgcgat
     2641 cgtggcgggc gacgccgaga tcgcttccat catggccatc aggccacctt cgatccgctt
     2701 ggcgatctcg atttcgcctt cgcgcgtcag cagttccacc gtgcccattt cgcgcatgta
     2761 catgcgcacc ggatcggtgg tgcggccgaa ctcgctgtcc accgtggaca gggccgcttc
     2821 ggcttcctct tcggcctctt cctcggtcgc cgccgtcgga ccggtgttgg tcagcagcag
     2881 tgtctcggcg tccggcgtct gctcgtagac ggccacgccc atgtcgttga gcatggagac
     2941 cacgacttcc agggtctcgg cgtccaccag cttttcgggc aggtggtcgg tgatctcggc
     3001 atgcgtgagg tagccgcggg tcttgcccag cttgatcagg gtcttcaggc gctggcggcg
     3061 cttgagcatt tcctcttcgg tgaggatggc ctcgtccagg ccgaattcct tcatcaaggc
     3121 ccgttccttg gccttgctga tcttcatgcg cagcggtttg accttttcgg tggtttcggc
     3181 caccggttca ccgaccagat cctcctcgat gtccgacagg tcgaggtctt ccacggtctt
     3241 gccttcgacc gcgtccttgg gcttgcggcc gcgcttggcg ccggctttgg cgggcgcggc
     3301 ggattccgcc gactgggcct tgggcgggcg gcccggcttt ttcttcacca cctcttcggc
     3361 ggcgctggcc ttgtggggtt tgttcacctc atccttggca ttggctttct cggatggctt
     3421 ggcaggggac ttcgtaaggg gcactggctt gactttcgtt gcgggcatag cgggggcctt
     3481 ggcctgcggc ttgggcgcgg tcttgacgac cttgagcttc gccttgccga ggacccccgg
     3541 tttgatcact tttgcggcgg caggtttcgc cgtgggcttc acgagctttc cggacttttg
     3601 agcgggcatg aaaaaatacc tcggaacttc aaaaagcaga aacacaaaaa agcgccacga
     3661 cggcccggcg ccggaggagg ggaaccctga aaaccccagg agggttttca actgtccttc
     3721 aaggggcaag atggatgggc gaacatttgg ggtgcagccc ttgcggtgag gtggcgggtc
     3781 gggagcaaat gtccgcggtt ggccgggtcc ggaggtgctg ctgtcgctct tgccgctagc
     3841 aaaccctaca ttatacggtt accgccgatt ttcaagggtt tggtgctttt tgagcgcgaa
     3901 ctgccggcgg atcagctgtt cctcttcggc ggcccggccg gggtccgtgg acggcagcgc
     3961 ctggacccgc ttcagttcca ggtccagcac gtcgagctgg agcttcacga cggcggcatg
     4021 gaagtcggcc aaaggcattt ccgggtcttc caccatccgg ccggaaggct ggaggttgtc
     4081 cagcgcgatg atccggcgcg ccaccgcctc gaaaggctgg ccttccagcc ccacggccag
     4141 cgcggccagc ggttgtggcc catgctcgtg ggcctggtag tcgatccacc ggaacaggtc
     4201 gccgaaatcc ccgtccaggg cgcacaggat ctcgtggtcg cccgagctga ggacttccca
     4261 cagggccgaa tcagccagca acaggcgggc gatgttgctc gcgctggctt ctattgaggg
     4321 gcggatggcc aggcgccggg agcgcggcgt caagccgctg ttcgggctgc gccgcaagtt
     4381 cccgcccggt cgtcccgcgg cgcccgagga ccacagcgaa ccgagttcgc gagcctccag
     4441 ctgcacgtgc cctgcgattt caccgagcaa ttgcctgcgc agcgcgccct cgggcagcag
     4501 gctccacagc ggcctggcat tcgcgaccat gcgcgcgcgt ccttcggccg tgccgatgtc
     4561 gcagccttcg cgcgaagctt cgacgaggaa ccgcgacagc ggcgtcgcct cgctcacata
     4621 gcgcgaaaac gcctccctgc cgaattcgcg gatgtaactg tcggggtcgt gttcggcggg
     4681 caggaacagg aatttgacgc tgcgcacgtc ggtggcatag ggcagggcgg cgtcgagcgc
     4741 cttgcgcgcg gcgcggcgtc cggcttcgtc gccgtcgaaa ctgaacacca ccgagtcggt
     4801 gaaacgaaag agcttgtgca cgtgttcggc cgtgcacgcg gtaccgagcg tggccaccgc
     4861 gttgggaaaa ccgagctgag ccagcgccac cacgtccatg tagccttcgg tcaccagcac
     4921 atagccgtgc tcgcgcaggg cctcgcgcgc ctcgaacagg ccgtagagtt cgcggccctt
     4981 gctgaacacc ggcgtctccg gtgagttcag gtatttcggt gttccctctc ccagcacccg
     5041 gccgccgaag ccgatgcatt cgcccttgac attgcgaatc ggaaacatca cgcggtcgcg
     5101 gaagcggtcg tagcgcttgt cctcgtcctc gttgacgatc acgaggccgc tttcggccag
     5161 caggggatcg tcgtagttgg gaaagacact ggccaggcta tgccagccct ccggcgcata
     5221 gcccaggccg aaaagctttg caatgccacc cgacaccccg cgccccttga ggtagtccac
     5281 cgcccgcggc gactccttca ggtgcttgcg ataggcgtcg caggctttct ccagcacatc
     5341 ggtcagcgtc gcctgcttct cgcgctgctg tgcggcccgg gcgcggtcct gcggcgaggc
     5401 atcctcctca ggcacctgca tgccgtactg ctgggccagg tccttggtgg cctcgacgaa
     5461 gttcatgccg gcgtgttcca tcaggaaacg gatcgcgtcg ccgttcttgc cgcagccgaa
     5521 gcagtggtag aactgcttgg tggggctgac tgaaaaggac ggcgactttt cgccatggaa
     5581 cgggcacagt cccatgtagt tcgcgccgcc tttcttcagc tgcacgtagc ggccgacgat
     5641 ttcgaccacg tcggcgcggg ccagcaactc ctggatgaaa gactggggaa tggccattgt
     5701 tgccgtattc ttacttaatg ctgcgccgca tcggaaaccg ttaatctcac aaggccgccc
     5761 aatgcctttt ccggggaact gaaaaacgtt ggcaagcccc atacttcgga ccaccgggaa
     5821 tatcggagac aagacatgcc ccacattttc gaccaggacc taccgcgcaa ccaggcgaat
     5881 ttcacgccgc tgtcgccgct ttccttcatc gagcgcaccg ccgaggttta tccgcaacgt
     5941 ctggcggtgg tgcacggcga cctgcggcgc aactggcgcg agctttacgg ccgctgccgt
     6001 cagctggcca gcgccttggc ccgaagcggc gtgggcaagg gcgataccgt cgccgtgatg
     6061 ctgcccaata cgccgccgat ggtggaagcg catttcggca tcccgatggc gggtgcggtc
     6121 ctcaacgcca tgaatacgcg cctggatgcc cagaccgtcg ccttcatgct ggatcacggc
     6181 gaagccaagg tggtgatcgt ggacccggag ttcgccggcg tgatgaaaga cgccatcgcg
     6241 ctgcgccagg aaaagcgccc gctgctggtc gtcgatgtcg aggatgcgtt gtacacgggt
     6301 ccggtcgagc gcgccggcac cgtcggctat gaggctttcc tggccggcgg cgacccggct
     6361 ttcgaatgga agctgccggc cgacgagtgg gacgcgatcg cgctgaacta caccagcggc
     6421 accacgggca acccgaaggg ggtcgtttac agtcaccgcg gggcgaccgc gaatgcgatc
     6481 tcgaacatcc tcgagtggga catgcccaag cacgccgtgt acctgtggac gctgcccatg
     6541 ttccactgca acggctggtg ctttccctgg accgtcgcgg cgcgcgccgg cgtcaacgta
     6601 tgcctgcgca aggtggaagc caaagcgatc ctcgacgcga tcaaggccca cggcgtgacg
     6661 cattactgcg gcgcacccat cgtgcagggg atgctggtga acgcgcccga ggagatgaag
     6721 cagggcctgc ccacgggcgt caaggcaatg gtggcgggag cggcgccccc ggcttccatg
     6781 atcgagggca tggagcgcat gggcttcgac ctcacccacg tgtatggcct caccgaggtc
     6841 tatggcccgg ccacggtgtg ccccaagcac gacgaatgga acgacgtgga catcggcgag
     6901 cgcgcgcggc tcaatgcccg ccagggcgtg cgctaccacc tgcagcgcga tgcgcgggtc
     6961 atcgatcccc agacgatgaa gccggtgccc atggacggcg agaccatggg cgagatcatg
     7021 ttccgcggca acatcatgat gaagggctat ctgaagaacg cggcggcgac gcaggaggcg
     7081 ttcgccggcg gctggttcca caccggggac ctggcggtgc agtacccgga cggctacatg
     7141 aagatcaagg accgcagcaa ggacatcatc atctccggcg gcgagaacat ttcctccatc
     7201 gaagtcgagg acgtgctgta ccgccatccg gacgtgctgg ccgcggctgt cgtcgccaga
     7261 ccggacccgc gctggggcga aacgccctgc gccttcatcg agctcaaggc cggcgcgacc
     7321 gccacgcgcg aggacatcgt cgcgcattgc aagaagcacc tggccggctt caaggtgccc
     7381 agggccgtcg tattcggtga gttgcccaag acctcgaccg gcaagatcca gaagttcgag
     7441 ttgcgcaaac aggcggggtc ggccgaggcg atcgacgttt gatcggctgc taccccaggg
     7501 ggtgcgccaa aagtcacgta taaaaacagc cccgcgtgcg accgcggctg gggttgcata
     7561 ctgtccgcat gcccatcctc agtcatcaag cggcgcacgt cgtagcgcat cccggacgct
     7621 ttgcctggtc ggtgctcaag gcgttcaagg ccaaccaggg cctgctgctg gccggtgcgg
     7681 tggcctacta cgcgctgctc tcggtggtgc cgctcctgat cctgaccgtg attgcgctgt
     7741 cgcacgtcat cgaccaggca gagctgttgc aaactctggg ccgctacctg gagtggctga
     7801 tg
//
LOCUS       DASWIY010000427         8064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2897451, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000427 DASWIY010000000
VERSION     DASWIY010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2897451"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VGA59_15205"
     CDS             complement(<1..821)
                     /locus_tag="VGA59_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX9721065.1"
                     /translation="MTSAAAALRPAVPDLLALRDDYRARKNELLACLKARGSSARGIR
                     SLLQSLAREADVTLKSLWERAGLPAGFALLAVGGFGRGELFPHSDVDVLVLMPDTAST
                     DGDSELKRKVEAFIGNCWDTGLEIGSSVRTASECVAQAERDVTVQTSLLESRLVTGDA
                     RLYAGFQMRFAATVDAKAFFIAKTLEMRQRHTKFENTPYSLEPNCKESPGGLRDLQVI
                     LWVAKAAGLGKSWDELARKGLATAFEARQIKRNEDLLSLIRARLHVISNRREDRLV"
     gene            complement(818..1621)
                     /gene="map"
                     /locus_tag="VGA59_15210"
     CDS             complement(818..1621)
                     /gene="map"
                     /locus_tag="VGA59_15210"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX9721066.1"
                     /translation="MSITYKDADGIQGMRVAGRLAAEVLDYITPHIKPGITTREIDRL
                     AAECMKKQGTTSATLGYQPAGYPPYPASLCTSVNHVVCHGIPNDKPLKKGDIVNVDVT
                     VIRDGWFGDTSRMFMVGEVSIAAKRLCALTYDAMWHGIVHVKPGIHLGDIGFAIQKFA
                     EGNGFSVVREFCGHGIGRNFHEEPQVLHYGKPGTLDELRPGMTFTIEPMINAGRREVK
                     EFGSDGWTIVTKDHSLSAQWEHTILVTETGYQVLTLSAGSPPPPPFVNA"
     gene            1798..5952
                     /gene="purL"
                     /locus_tag="VGA59_15215"
                     /gene_synonym="purI"
     CDS             1798..5952
                     /gene="purL"
                     /locus_tag="VGA59_15215"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX9721067.1"
                     /translation="MTLHITEFEGGNALSDFRVQQLLPRLQAIDGKVSGISARFVHLV
                     ATDHTPGPAERERLAALLTYGEPCRTDAQGSLIVVTPRFGTVSPWASKATDIAHNCGI
                     ALKRVERITEFRVGTWSPLLGKSAMDATGRLAIAALLHDRMTESVMFERSAAHGLFTE
                     LAPAPMEHVDVLGGGAAALERANTQFGLALAADEIEYLVRAFKGLGRNPSDVELMMFA
                     QANSEHCRHKIFNASFAIDGVAQERSMFGMIRHTHQVSPQHTVVAYSDNASVMEGGSV
                     ERFIARPGAGSRYRKEAAVDHVLMKVETHNHPTAISPFPGAATGAGGEIRDEGATGRG
                     SRPKAGLTGFTVSRLWGGSFGKPGHIASALQIMVEGPLGGAAFNNEFGRPCLLGYFRE
                     YEQAIRYRLAGEQQEREELRGYHKPIMIAGGLGTIAANLTKKIEFPAGTLLIQLGGPG
                     MRIGMGGGAASSMATGTNVAELDFDSVQRGNPEIERRAQEVINHCWAEGEANPILAIH
                     DVGAGGLSNAFPELTNDAGRGARFDLRKIPLEESGLAPKEIWCNESQERYVLAIAAQS
                     LEQFRAFCERERCPFAVVGVATHERQLVVADADLPLTPTRPLASLSPTGRGSDSHPLP
                     PERGRDLLPLPRGEGGGEGMAVDMPMDVLLGKPPKMHRDVKTVRREFKPVQLTGVSLQ
                     DAAIKVLGHPTVASKRFLVTIGDRTVGGLSHRDQMVGPWQVPVADCAVTLADYKGFAG
                     EAMSMGERTPLAAIDAPASGRMAVAEAITNLLAAPIELARVKLSANWMAACGEPGEDA
                     ALYETVRAVGMELCPALGISIPVGKDSLSMRTQWTEPAGTSSGANAVKKVTSPVSLIV
                     SAFATLADVRATLTPQLDATLDTTLVLVDLGRGRNRMGGSILAQALDQMGHEVPDLND
                     PEDLVRLVNAVNALRAQSKILAYHDRSDGGLFAAACEMAFAGHVGVSLNVDLLVTEGD
                     GITDGRADYGDAKNWASQVGARREELTLKALFNEELGVLLQVRTDGRDQVMQLLREHG
                     LAKYSHFVGTTRPASSAVDAGKGEVQVWRDTKAVFSAGLADLHQVWDSVSWKICRQRD
                     NPAGADAEHTAAGDPADPGLQVLPAPMLQEARAEGAAPYLNLARPKVAVLREQGVNSH
                     VEMAYCFTEAGFDAYDVHMTDLQAGRARLEDFKGFVACGGFSYGDTLGAGIGWARSIT
                     FNARLADQFKGFFGRDDTFALGVCNGCQMLAELADIVPGAQAWPRFTTNLSERYEARL
                     SQVEVLPSPSLFFAGMAGSRLPIAVAHGEGYANFGQRGDKGRVIAAMRFTDNHGIPTE
                     AYPFNPNGSPAGLTAVTTADGRFTAMMPHPERVFRNIQMSWTSGDRDALSPWMQIWRN
                     ARKWVG"
     gene            6037..6804
                     /locus_tag="VGA59_15220"
     CDS             6037..6804
                     /locus_tag="VGA59_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEX9721068.1"
                     /translation="MKRELRPLRLTDPLRWLRAGWGDLMAAPGIALFYGLAFWLMAVI
                     LGAVFRNMPQYTISFVSGCFLVGPFLAMGLYEVSRRREQGLPQSLAASLTCWDRHLRS
                     MGMLVLVLLLLELLWGRASLVVFAVFFNTAMPTTAGILQAVYNPKNWQFIAVYLAVGG
                     VFAALVFSTCVVSLPMILDRDTDAVSAGLASIQVVALRTGPMLVWGALIVALVLGALL
                     LPWAMALLVVGPWLGHASWHAYRGCLAWRPVEAAPDA"
     gene            complement(6824..>8064)
                     /locus_tag="VGA59_15225"
     CDS             complement(6824..>8064)
                     /locus_tag="VGA59_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX9721069.1"
                     /translation="MAAQGLRVLAVAQRSHDRLPQEGDGIDAVESGLCLIGLVGLIDP
                     PRPEAAAAVRECMDAGITPVMITGDHPATARAIALRLGLVKDPRAPVLTGAELATLDE
                     DALLARARHLRIYARVDPAQKIRIIQALQAQGEFVAMTGDGVNDAPALKRADIGVAMG
                     KGGTDVAREASSLVLLDDNFATIVAAVREGRRIYDNIRKFVRYAMTGNSGEIWVIFLA
                     PLMGLPIPLLPIHILWINLVTDGLPGLALAAEPAERGVMQRPPRPPGESLFARGMWQH
                     ILGVGLLLGGLCLAVQAWALSTGHAHWQTMVFTVLTFGQMAHVLAIRSEEQPLWRLGL
                     GSNKPLLGAVLLTFALQLATIYVPALNPVFKTQPLSAGELAICVATAAVVWVAVEIEK
                     AWRRRRHLPAGEAAADAPAR"
BASE COUNT         1316 a         2705 c         2733 g         1310 t
ORIGIN      
        1 accaggcggt cttcgcgccg attggaaatc acgtgcaggc gggcgcggat caggctcagc
       61 agatcttcgt tgcgcttgat ctgccgggcc tcgaacgccg tggcgagccc cttgcgcgcc
      121 aattcgtccc agctttttcc caggcccgcg gccttggcca cccacaggat cacctgcagg
      181 tcgcgcaagc ccccgggcga ttccttgcaa ttgggttcca gggaataggg cgtgttctcg
      241 aacttggtat ggcgctggcg catctccagc gtcttggcga tgaagaacgc cttggcgtcc
      301 acggtggcgg cgaagcgcat ctggaaaccg gcatagagcc gggcatcgcc ggtcaccagg
      361 cgcgattcca gcagagacgt ctgcaccgtg acgtccctct cggcctgggc cacgcactcg
      421 gacgccgtgc gcacgctgga accgatctcc agaccggtgt cccagcaatt gccgatgaat
      481 gcctcgacct tgcgcttgag ttcggaatcc ccgtcggtgg aggcggtgtc gggcatcagg
      541 accagcacat ccacgtccga atgcgggaac agctcgccgc gcccgaaacc gcccaccgcc
      601 agcagcgcga agccggcggg aagtccggcc cgctcccaca atgacttcag cgtgacgtcc
      661 gcctcgcgtg ccagcgactg cagcaggctg cggatgccgc gcgccgagct gccgcgcgcc
      721 ttcaggcagg ccagcagctc attcttgcgc gcgcggtagt cgtcgcgcag tgccaggaga
      781 tcgggcacgg ccgggcgaag cgcagcggcc gcggaggtca tgcgttgacg aaaggcggtg
      841 gcggcgggct gccggccgag agcgtcagca cctgatagcc ggtttcggtg accaggatgg
      901 tgtgctccca ctgggccgac agcgaatgat ccttggtgac gatggtccag ccgtcgctgc
      961 cgaactcctt cacttcgcgg cgcccggcat tgatcatggg ctcgatggtg aaggtcatgc
     1021 ccggcctgag ctcgtccagt gtcccgggct tgccatagtg cagcacctga ggctcttcat
     1081 ggaaattgcg gccgatgccg tggccgcaga actcgcgcac gaccgagaag ccgttgccct
     1141 cggcgaattt ctggatggcg aagccgatgt cgcccaggtg aatgccgggc ttgacgtgca
     1201 cgatgccgtg ccacatcgcg tcgtacgtca gcgcacagag ccgcttggcg gcgatggaca
     1261 cctcgccgac catgaacatg cggctcgtgt cgccaaacca gccgtccctg atcacggtga
     1321 cgtcgacgtt gacgatatcg cctttcttca gcggcttgtc gttgggaatg ccgtggcaca
     1381 ctacatggtt gaccgatgtg cagagggaag ccgggtaggg cgggtagccg gcaggctggt
     1441 aacccagcgt ggccgaggtc gtgccttgct ttttcatgca ctctgctgcc agccggtcga
     1501 tctcccgggt ggtaattccc ggcttgatat gcggcgtgat gtagtccagc acctcggcgg
     1561 caagccttcc tgccacgcgc atgccctgga taccgtcggc gtctttgtag gtaatgctca
     1621 ttgattcaat tatcccagac agtgaattcc cttgcctcgg gagccggtgc gttcccgccg
     1681 gggacggcaa accgcccgca gcgcttgcta tgccccagtt aaaataggca ggtcaacacg
     1741 ggggccccag tcagcggcct gccgttcacc gtttccccgt ttcacacagg tttcacagtg
     1801 accttgcaca tcaccgagtt cgagggcggc aacgccctca gcgacttccg agtccagcag
     1861 ctacttcccc ggctgcaagc catcgatggc aaggtcagcg gcatcagcgc ccgtttcgtg
     1921 cacctggtgg ccaccgacca tacgcccggc ccggccgagc gggagcgcct ggccgcgctg
     1981 ctgacctacg gcgagccctg ccggaccgac gcgcagggtt ctctgattgt cgtgacacct
     2041 cgtttcggca cggtttcccc ctgggcctcg aaggccaccg atatcgcgca caactgcggc
     2101 atcgcgctca agcgggtgga gcgcatcacg gaattccgtg tcgggacttg gtctcccctg
     2161 ctcggcaaga gcgcaatgga cgcgaccggg cggctcgcca tagcggccct gttgcatgac
     2221 cgcatgaccg aaagcgtgat gttcgagcgc tccgccgcgc atggcctgtt taccgagctg
     2281 gccccggctc cgatggagca cgtcgatgtc ctgggcggcg gcgcggcggc gctggagcgg
     2341 gccaacacgc aattcgggct ggccctggcc gctgacgaga tcgagtacct ggtccgggct
     2401 ttcaagggcc tgggccgcaa cccgagcgac gtcgagctga tgatgttcgc gcaggccaac
     2461 agcgagcact gccgtcacaa gattttcaac gcgtcgttcg ccatcgacgg cgtggcgcag
     2521 gaacgcagca tgttcggcat gatccggcac acccaccagg tgagcccgca gcacacggtc
     2581 gtcgcgtact cggacaacgc gtcggtcatg gaaggcggca gcgtggagcg tttcatcgcg
     2641 cggccgggtg ccggatcgcg ctaccggaag gaagccgccg tcgatcacgt gctgatgaag
     2701 gtggaaaccc acaaccaccc gacggcgatc tcgccttttc ccggcgccgc cacgggcgcg
     2761 ggaggcgaga tcagggacga gggcgcgacc ggccggggct ccaggcccaa ggccggtctc
     2821 acgggattca cggtttccag gctgtggggc ggcagcttcg gcaaacccgg gcacatcgcg
     2881 agcgccctgc agatcatggt ggaaggcccg ctcggaggcg cggccttcaa caacgaattc
     2941 ggccggcctt gcctgctggg ctatttccgc gaatatgagc aagccatccg ttaccggctg
     3001 gccggcgagc agcaggaaag ggaagagctg cgcggctacc acaagcccat catgatcgcg
     3061 ggcggcctgg gcacgatagc cgccaacctg acgaagaaga tcgagttccc ggccggcacg
     3121 ctgttgatcc agctgggcgg gcccggcatg cgcatcggca tgggcggcgg cgcggccagt
     3181 tccatggcca ccgggaccaa tgtggccgag ctcgatttcg attcggtgca gcgcggcaac
     3241 ccggagatcg agcggcgcgc ccaggaggtc atcaaccatt gctgggccga gggcgaggcc
     3301 aaccccattc tcgcgatcca cgacgtgggc gccggcggcc tgtccaatgc cttccccgaa
     3361 ctgaccaacg acgccggccg cggcgcgcgg ttcgacctgc gcaagatccc gctggaagaa
     3421 tccgggctcg cgcccaagga aatctggtgc aacgagagcc aggaacgcta cgtgctggcg
     3481 atcgctgcgc agtcgctcga gcagttcagg gctttctgcg agcgcgagcg ctgcccgttc
     3541 gccgtggtcg gggtggcaac gcacgagcgg cagctggtag ttgccgacgc cgatttgccc
     3601 ctcaccccaa cccggccgct cgcttcgctc tccccaacgg ggagagggag tgattcacac
     3661 cctctccctc cggagagagg gagagatttg ctccctctcc ctcggggaga gggcgggggt
     3721 gagggcatgg ccgtcgacat gccgatggat gtcctgctgg gcaagccgcc caagatgcac
     3781 cgcgacgtga agaccgtcag gcgcgagttc aagccggttc agctcaccgg cgtcagcctg
     3841 caggatgcgg cgatcaaggt cctggggcac ccgacggtgg cgtccaagcg tttcctcgtc
     3901 accatcggcg accgcaccgt gggcgggctg tcgcaccgcg accagatggt cgggccctgg
     3961 caggtgccgg tggccgactg cgccgtgacg ctggccgact acaagggctt cgcgggcgaa
     4021 gcgatgagca tgggggagcg cacgccgctt gccgccatcg atgcgccggc ttcgggacgc
     4081 atggcggtgg ccgaggccat caccaacctg ctggcggcgc cgatcgagtt ggcgcgcgtc
     4141 aagctgtcgg cgaactggat ggcggcctgc ggcgagcccg gcgaggacgc cgcgctctac
     4201 gaaacggtga gggcggtggg catggagctg tgccctgcgc tgggcatttc gattccggtc
     4261 ggcaaggaca gcctgtcgat gcggacgcag tggacagaac ccgcggggac atcatcgggt
     4321 gcgaacgcag tgaagaaagt cacctcgccg gtgagcctga tcgtcagcgc cttcgccacc
     4381 ctggccgacg tgcgcgcaac gctcacgccc cagctggacg cgacgctgga cacgacgctc
     4441 gtcctggtcg acctgggccg gggccgcaac cgcatgggcg gcagcatcct ggcccaggcg
     4501 cttgaccaaa tgggccacga ggtgccggac ttgaatgacc ccgaagacct ggtaaggctc
     4561 gtcaacgccg tgaacgcgct gcgagcccag agcaagatcc tggcctacca cgaccgcagc
     4621 gacggcggcc tttttgctgc cgcctgcgag atggcgttcg ccggccacgt cggcgtttcg
     4681 ctcaacgtgg atctgctggt caccgagggc gacggcatca ccgacggccg cgccgattac
     4741 ggcgacgcca agaactgggc gagccaggtc ggtgcgcggc gcgaagaact caccctcaag
     4801 gcattgttca atgaagagct cggcgtgctg ctccaggtgc gcaccgacgg ccgcgaccag
     4861 gtcatgcaac tgctgcgcga gcacggcctc gcgaagtaca gccatttcgt cggaacgacc
     4921 cggcccgctt cgtccgccgt cgacgccggc aagggcgaag tgcaggtctg gcgcgacacc
     4981 aaggcggtgt tttccgccgg gctggccgac ctgcaccagg tctgggacag cgtgagctgg
     5041 aagatctgcc ggcagcgcga caacccggcc ggcgccgatg cggaacacac cgccgccgga
     5101 gacccggcgg acccgggttt gcaggtcctg ccggctccaa tgctgcaaga ggcgcgggct
     5161 gaaggcgccg caccgtacct gaacctggcc cggcccaagg tcgcggtcct gcgcgagcag
     5221 ggcgtcaatt cgcacgtcga gatggcgtac tgtttcaccg aggccggctt cgacgcctac
     5281 gacgtccaca tgaccgacct gcaggccggc cgcgccaggc tggaagactt caagggcttc
     5341 gtggcctgcg gcggcttcag ctacggcgac acgctcgggg cgggcatcgg ctgggcccgc
     5401 agcatcacct tcaatgcaag actcgccgac cagttcaagg gctttttcgg ccgcgacgac
     5461 accttcgcgc tgggcgtatg caacggctgc cagatgctgg cggaactggc cgacatcgtg
     5521 ccgggggcgc aggcctggcc gcgcttcacg accaacctga gcgagcgcta cgaagcgcgc
     5581 ctgtcgcagg tggaagtgct gccctcgccc agcctgttct ttgccggcat ggcgggcagc
     5641 cggttgccca ttgccgtggc gcacggcgag ggttatgcga acttcggcca acgcggggac
     5701 aagggcaggg tcatcgccgc gatgcgcttc accgacaacc acggcatccc gaccgaggcc
     5761 tatcccttca acccgaacgg aagtccggcc ggactgacgg ccgttacgac ggccgacggc
     5821 cgcttcaccg ccatgatgcc gcacccggag cgcgtgttcc gcaatatcca gatgagctgg
     5881 acctcgggtg accgtgacgc gctcagtccc tggatgcaga tctggcgcaa tgcgaggaag
     5941 tgggtggggt aagtggacac tggaatgccg ccacctgacg aacccggcag taccgctgct
     6001 tcttccgccg ccgtggcgga ccggcaggcg cagccgatga agcgagaact gcggcccttg
     6061 cggctgacgg atccgctgcg gtggctgcgc gccgggtggg gcgacctgat ggcggcaccg
     6121 ggtatcgcgc tgttctatgg cctggccttc tggctgatgg ccgtaattct gggcgccgtg
     6181 ttccgcaaca tgccgcagta cacgatctca ttcgtgtcgg gatgctttct ggtgggaccg
     6241 ttcctcgcca tgggcctgta cgaagtgagc cgccggcgcg agcaggggct gccgcaaagc
     6301 ctggccgcat cgctgacctg ctgggaccgg cacctgcgca gcatgggcat gctggtgctg
     6361 gtgctcctgc tgctggaact gctgtggggt cgcgcttcac tggtggtgtt cgcggtgttc
     6421 ttcaacaccg ccatgccgac cacggccggc atcctgcaag ccgtgtacaa cccgaagaac
     6481 tggcaattca tcgccgtgta cctggccgtg ggcggggtct ttgccgctct ggtgttttcg
     6541 acctgcgtgg tgtcgctgcc gatgatcctg gaccgcgaca ccgacgctgt ttccgccggc
     6601 ctcgccagca tccaggtggt ggccctgcgc accgggccca tgctggtctg gggcgcgctg
     6661 atcgtggccc tggtgctggg cgcgctgctg cttccctggg cgatggcgct gctggtggtg
     6721 ggaccttggc tgggccatgc cagctggcat gcgtaccgcg gctgtcttgc ctggcggccc
     6781 gtcgaggcgg cgccagacgc ctgagctttc aacgcggggg gcctcaacgg gcaggcgcat
     6841 cggccgcggc ctcacccgcc ggcagatgcc tgcggcgacg ccatgccttc tcgatctcca
     6901 ccgcgaccca caccaccgcg gccgtcgcga cgcagatggc cagttcgccg gccgacagag
     6961 gctgggtctt gaagacggga ttgagcgcgg gcacgtagat ggtggccagc tgcagcgcga
     7021 aggtgagcag taccgcgccg agcaatggtt tgttcgaccc cagcccgagc cgccacaatg
     7081 gctgctcttc cgaacggatg gccagcacat gggccatctg cccgaaggtc agcaccgtga
     7141 acaccatcgt ctgccaatgc gcgtgcccgg tggacagggc ccatgcctgc accgccaggc
     7201 acaggccgcc caggagcagg ccgaccccca ggatgtgttg ccacatgccg cgcgcgaaca
     7261 ggctttcccc aggggggcgc ggcgggcgct gcatgacgcc gcgctcggcg ggttcggccg
     7321 ccagcgccag cccgggcagc ccgtcggtca ccaggttgat ccacaggatg tgaatcggca
     7381 gcagcgggat cggcaggccc atcagcggcg ccaggaaaat gacccagatc tcgcccgagt
     7441 tgccggtcat ggcatagcgc acgaacttgc ggatgttgtc gtagatgcgc cggccttcgc
     7501 gcaccgcggc cacgatggtg gcgaagttgt cgtccagcag gaccaggctg gacgcttcgc
     7561 gcgccacgtc ggtgccgccc ttgcccatgg ccacgccgat gtcggcgcgc ttgagcgcgg
     7621 gagcgtcgtt cacgccgtcg ccggtcatgg ccacgaattc accctgcgct tgcagcgcct
     7681 ggatgatccg gatcttctgc gccgggtcga ctcgcgcgta aatgcgcaaa tggcgcgcgc
     7741 gcgccagcaa cgcgtcctcg tcaagagtcg ccagttcggc ccccgtgagg accggggccc
     7801 gcggatcctt gaccagcccc aggcgcaacg cgatcgcccg ggccgtcgcc ggatgatcgc
     7861 ccgtgatcat caccggtgtg atgccggcgt ccatgcattc acgcacggcc gccgcggctt
     7921 cgggccgggg cgggtcgatc agcccgacca gcccgatcag gcaaaggccg ctttccaccg
     7981 cgtcgatccc atcgccctct tgcggcaatc ggtcatggct gcgctgcgcg acggccagca
     8041 cccgcaatcc ctgcgccgcc atgg
//
LOCUS       DASWIY010000428         6298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1800027, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000428 DASWIY010000000
VERSION     DASWIY010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800027"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..487)
                     /locus_tag="VGA59_15230"
     CDS             complement(311..487)
                     /locus_tag="VGA59_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721070.1"
                     /translation="MTPLDKLASLPKAATFLREGVTFEDLHALARALTDVQAAEELNE
                     ARNALFRRVPKKTA"
     gene            903..2096
                     /locus_tag="VGA59_15235"
     CDS             903..2096
                     /locus_tag="VGA59_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX9721071.1"
                     /translation="MSSYIWQKPGWPGFTCDLEAASAALVEARRMQGEIEGKARAVGI
                     DGTGEMVREVLAQEVIATAAIEGEKLDPAAVRSSVMRRLGLADVGPHDRHVDGLVEVI
                     SDAGTAFDTPLDHDRLWRWQSALFPGGTSGIRRIAVGRYRDHPDPMQIVSGMPGKEIV
                     HYEAPPSSRVPAEMERFVTWFSSTTPRPGQPPKMDGLARAAIAHLWFESIHPFEDGNG
                     RIGRAILDMAIAQDHRAPVRLYSLSRQLLESRGGYYDALNLAQHGDGNVTQWVTWLSQ
                     KFCAACLRSSQIIDRALEKARFWADHSGAELNARQRKALQRLLDDGDGGFSGGLNAEK
                     YIKMTGTSKPTATRDLTYLVEGNLLWTVGQGKAVRYYVNVPGWTHSVAPQEEQPGRPP
                     ERPKG"
     gene            complement(2199..3116)
                     /locus_tag="VGA59_15240"
     CDS             complement(2199..3116)
                     /locus_tag="VGA59_15240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX9721072.1"
                     /translation="MAVYSMTGYASAQQSGAQAASGQDAKGVAASRLGLEIRSVNSRF
                     LDLSFRLSEELRQHEPALREMITARLKRGKVEIRAALETAAGDNLREPAPRLLQRLNA
                     LQDTVRAWLPDAQALGVADVIRLSAAEGSQDTDWAPALGRLATTTLDALMAAREREGA
                     RLAAMLLGHLKQLRELALQARPLVPQLVEQQRTRFLERWKEAMALAEGVSLPEAAQDR
                     ALSEATAFAIRIDVAEELMRLGSHIDEIERLVNKGGEIGKRLDFLIQELHREANTLGS
                     KSAALELTHISVDMKVLIEQMREQVQNIE"
     gene            3214..4227
                     /locus_tag="VGA59_15245"
     CDS             3214..4227
                     /locus_tag="VGA59_15245"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9721073.1"
                     /translation="MSKLKPAPLPPDTVIGGYRVVRKLSAGGFGVVYLAVDNEGQQVA
                     VKEYLPASLATRSPGELLPQVQPEKLSLYRLGLKSFFEEGRSLAQISHASVVSVLNFF
                     RENETVYMVMNYLEGATLQDFIITARELKKQKVFRESTIRSLFDEILRGLRIVHQHKM
                     LHLDIKPANIFITDDNKAVMIDFGAAREVLSKEGNFIRPMYTPGFAAPEMYRRDSSMG
                     PWTDIYAIGACIYACMQGYPPNDAPQRLEKDRLALALSRLRGVYSDNLIEVVEWCMSL
                     DPLSRPQSVFALQKELSREGERRYTKLSVGEKMRLQFDNMVSDTKKNVKRATGFGARA
                     AKP"
     gene            4224..5129
                     /locus_tag="VGA59_15250"
     CDS             4224..5129
                     /locus_tag="VGA59_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX9721074.1"
                     /translation="MKFSVYQVSRKGGREKNEDRMGYCYTRESGLFVLADGMGGHPEG
                     EVAAQLALQTISALYQKEARPIVKDVADFLSTSLMAAHHQVIRYASEKGMLDTPRTTL
                     VAAIVQGSYATWVHCGDSRLYVVRQGELLTRTRDHSYLEQQASSGVVRLDRINRNILF
                     TCLGSPTKPVFDVTGPITMQEGDKILLCSDGLWGAVSDIDIVRHLSMKPVSEAVPDLV
                     ETALRNGGAQSDNVTVIALEWETPDSFESTRGISTDSISDGVFASTIQAGVLDTMVED
                     LDDAAIERSIAEINEAIRRSAARKT"
     gene            complement(5388..6266)
                     /locus_tag="VGA59_15255"
     CDS             complement(5388..6266)
                     /locus_tag="VGA59_15255"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX9721075.1"
                     /translation="MAHELRAGQPDEGAQGLLGGRQLRVVDGSVVREPGVTGSQWRLH
                     YAIGLPSLQCQEVHLGPCDDGETLKRFQVHVGDIFVADRGYAHPGGIAHVRRHGGDVI
                     VRMNLVTLSLTHPSTGKPLDVLQQVRGLQVGQAGSWPACLKVKAERGSQRAQEIVGRL
                     CAVRKSAAAAAMGRDRVRRESMRGRRQLQPQTLEAAEYVLIFTTLGPDISAEQVMTLY
                     RLRWQIELEFKRLKSLIQLGHLKKHDARAARSWLQGKLLVALLIARLIAHSERVSPWG
                     YEFSWFDSSARALPVA"
BASE COUNT         1113 a         2074 c         2049 g         1062 t
ORIGIN      
        1 agtcacccca gtcttttcca attcaggtat gagcctgggc ggagcgcaaa gtagcggggc
       61 gaagtaggtc gatacgttgt agaggttgga agtgtaggcg tgccgggttt cgggggttct
      121 ttttcaacgg ctgttcggca agcgatcggc cccccaggcc gattgcgcgt caggccgtag
      181 gctccaggcc ggggagcggt ggtcaagccc ggcaccccga tgcgtcagcg aggggctgtc
      241 cacgcccttg tggtgtgggc agcgggcttg tccaccgcgg gcgggcgcgg gcaccgtgca
      301 aggcgggggc tcaggctgtt tttttcggca cgcgccggaa cagcgcgttg cgtgcttcgt
      361 tgagttcctc ggcggcctgc acatcggtga gcgcgcgagc gagcgcgtgc aaatcttcga
      421 aggtgacgcc ctcgcgcagg aacgtcgcgg ccttgggcag gctggccagc ttgtccagcg
      481 gcgtcattgc ggccctgggc cggtacaccc gcttgacgcg cccgggcttc ttggggcgcc
      541 cacgtcggtg gcgaacaggc agggccgatg gaagttcagg aacgggttga ggtaccccgg
      601 cagcatccgc acaacaggcc tcggcgcaaa ccgggcagac cggcacgagc gagggtgcgc
      661 ctggggtggc tcctgagggc aatgctgtcg gcgccggtgc ccagttgcca cagccctgtg
      721 cctgagcctg ggccacagcc acaaccaact atccacacca atcgacacaa aagcacggtt
      781 accccgggtc ggaaatatgt ggtccttttt cggacaaggg ctgaggctgg aaaggcgtcc
      841 gtgagccgca aatgccataa atacggctca ttgcgtgatc cgcattcgct agaattcggc
      901 tcatgagctc ctacatctgg cagaaacctg gctggcccgg gttcacttgc gacttggagg
      961 ccgccagcgc ggccctggtc gaggctcgcc ggatgcaggg cgaaatcgaa ggaaaagccc
     1021 gcgcggttgg catcgatggt accggtgaaa tggtccgcga agtgctcgcg caagaggtga
     1081 ttgccacggc cgccatcgaa ggcgagaagc tcgatccggc agcggtgcgg tcttccgtca
     1141 tgcgccgcct cgggttggcc gacgtcgggc ctcacgaccg gcatgtggac ggcctggtcg
     1201 aagtgatcag cgatgcgggc acagcattcg acacgccgtt ggatcacgac cggctttggc
     1261 gctggcagtc ggcgttgttc ccgggcggca cgagcggaat tcggcgcatc gcggtgggcc
     1321 gctaccgcga ccatccggat cccatgcaga tcgtgagcgg catgccgggc aaggagatcg
     1381 ttcactacga ggcgccgccg tcgagccggg tgcccgccga gatggagcgg ttcgtgacat
     1441 ggttctccag cacgacgccc aggccggggc agcccccgaa gatggacggt ctggcgcgcg
     1501 ccgcgatcgc tcacctctgg ttcgaaagca tccacccatt cgaagatggg aacggccgca
     1561 tcggcagagc gatccttgat atggcgatcg cacaggacca cagggcgccc gtgcggctgt
     1621 acagcctgtc ccggcagctg cttgaatcgc gcggcggcta ctacgacgcg ttgaacctgg
     1681 cgcagcacgg cgacgggaac gtcacgcaat gggtgacgtg gctctcgcag aagttctgcg
     1741 cggcctgcct tcgctccagc cagatcatcg atcgagcgct cgagaaagcc cggttctggg
     1801 ccgatcattc cggcgcggag ctcaatgccc ggcaaaggaa ggctctgcaa cgcctcctgg
     1861 acgacggcga tggcggcttc tccggcgggc tcaacgcgga aaaatacatc aagatgacgg
     1921 gtacgtcgaa gccgactgcc acgcgcgatt tgacgtacct ggtcgagggg aacctgctgt
     1981 ggaccgtggg gcaaggcaag gccgtgcgct actacgtcaa tgtgcccggg tggacacata
     2041 gcgttgcccc ccaagaagaa cagccgggca ggccgccgga gcgccccaag gggtagagcc
     2101 gcatgcagtc ctattcgccg ttggagtttt tgagatccga gggcttgaaa tcaacaactt
     2161 agacggtgtt tcggggtggt aagagacccc aagaaaccct actcgatgtt ctgcacctgc
     2221 tcgcgcatct gctcgatcag gactttcatg tcgaccgaga tgtgggtgag ctccagcgcg
     2281 gccgacttgg aacccagcgt attggcctcc cggtgcagtt cctgaatcag gaagtccagc
     2341 cgcttgccga tctcgccgcc tttattgacc agccgctcga tctcgtcgat atgcgagccc
     2401 agacgcatca gctcctcggc cacgtcgatg cggatggcga aagccgtggc ttcgctcagg
     2461 gcgcggtcct gcgccgcttc gggcaggctg accccctcgg ccagcgccat ggcttccttc
     2521 catcgctcca ggaagcgggt gcgctgctgt tcgaccagct gcggcaccag cggccgcgcc
     2581 tgcagcgcca gctcgcgcag ctgcttcagg tggcccagga gcatcgcggc cagccgcgcg
     2641 ccctcgcgct cgcgcgccgc catcaacgcg tccagcgtgg tggtggcgag ccgtcccagg
     2701 gccggcgccc aatcggtgtc ctggctgcct tcagcggcgg agagccggat gacgtcggcc
     2761 acgcccagcg cctgcgcgtc gggcagccag gcgcgcacgg tgtcctgcag ggcattgagc
     2821 cgctgcagca ggcgcggggc gggctcgcgc aggttgtcgc cggccgcggt ttcgagcgcc
     2881 gcgcggattt ccaccttgcc gcgtttgagc ctggcggtga tcatttcgcg caacgcgggc
     2941 tcgtgctggc gcaactcctc ggacaggcga aacgagaggt cgaggaagcg gctgttgacg
     3001 gagcggattt ccagcccgag ccggctcgcc gccacgccct tcgcatcctg tccggacgcg
     3061 gcttgcgccc cgctttgctg ggcgcttgcg tagccggtca tgctgtaaac tgccatcgag
     3121 gtgtttcaat cgttcggttc gttgcgggat tgcgagaata accgggttat cgagcggacc
     3181 cgatattccc cgccggccct acattcctaa attatgtcaa agctcaaacc agccccgctg
     3241 ccgccggaca ccgtcattgg cggctaccgc gtggtgcgca agctgtcggc gggcggtttt
     3301 ggcgtggtgt acctggccgt ggacaacgaa ggccagcagg tggccgtcaa ggaatacctg
     3361 ccggcatcgc tggccacccg ttccccgggt gagctgctgc cgcaggtcca gcccgaaaaa
     3421 ctctcgctct atcgcctggg tctcaagagc ttcttcgagg aaggcagatc gctcgcccaa
     3481 atttcgcacg cctccgtggt aagcgtgctg aatttctttc gcgagaacga aaccgtctac
     3541 atggtgatga actacctgga gggcgcgacc ctgcaggact tcatcatcac ggcgcgcgag
     3601 ctgaaaaagc agaaggtttt ccgcgagtcc accatccggt cgctgttcga cgagatcctg
     3661 cggggcctgc gcatcgtgca ccagcacaag atgctgcacc tggacatcaa gccggccaac
     3721 atcttcatca ccgacgacaa caaggccgtg atgatcgact tcggcgccgc gcgcgaagtg
     3781 ctgtccaagg aaggcaactt catccgcccc atgtacacgc ccggattcgc cgcgcccgag
     3841 atgtaccggc gcgactcgtc gatgggcccg tggaccgaca tttacgcgat cggcgcctgc
     3901 atctacgcgt gcatgcaggg ctatccgccc aacgacgcgc cgcagcggct cgagaaggac
     3961 cggctggcgc tggccctgtc gcgcctgcgc ggcgtctact cggacaacct gatcgaggtc
     4021 gtcgagtggt gcatgtccct cgatccgctg tcgcggccgc aatcggtgtt cgcgctgcaa
     4081 aaagagctca gccgcgaagg cgagcgccgc tacaccaagt tgtcggtagg cgagaagatg
     4141 cggctgcagt tcgacaacat ggtgtccgac accaagaaga acgtgaagcg cgcgaccggc
     4201 ttcggggcac gggcggccaa gccatgaaat tctcggtgta ccaggtcagc cgcaagggcg
     4261 ggcgcgagaa gaacgaagat cgcatgggct actgctacac gcgcgaatcg ggcctgttcg
     4321 tgctcgccga cggcatgggc ggccatcccg aaggggaggt ggcggcccag ctggcgctgc
     4381 agacgatctc ggccctgtac cagaaggaag ccaggcccat cgtcaaggac gtggccgact
     4441 tcctgtcgac ctcgctgatg gccgcgcacc accaggtcat ccgctacgcg agcgagaagg
     4501 gcatgctcga tacgccgcgc acgacgctgg tcgcggcgat cgtgcagggc agctacgcca
     4561 cctgggtcca ttgcggcgat tcgcgcctct atgtcgtgcg ccagggcgag ctgctgacgc
     4621 gcacgcgcga ccactcgtac ctcgagcagc aggccagctc gggcgtggtg cggctggacc
     4681 gcatcaaccg caatatcctg ttcacctgcc tgggctcgcc caccaagccg gtattcgacg
     4741 tcaccggccc gatcacgatg caggagggcg acaagatcct gctgtgctcc gacgggctgt
     4801 ggggcgccgt gagcgacatc gacatcgtgc gccacctgag catgaagccg gtgtccgaag
     4861 cggtaccgga cctggtggag acggcgctgc gcaacggcgg cgcgcagagc gacaacgtca
     4921 ccgtgatcgc cctggagtgg gaaacccccg actcgttcga atccacccgc ggcatctcca
     4981 cggacagcat cagcgacggg gtgttcgcct cgaccatcca ggcgggcgtg ctcgatacca
     5041 tggtcgagga tctggacgac gccgccatcg agcgttcgat cgccgagatc aacgaggcca
     5101 tccgccgttc ggcggcgcgc aagacctaga cattcccgcc cacggttgtc atcccggacc
     5161 ccggatcaag tccggggcag gcccgatccg caatccactt gcttcaaccc cggacttgat
     5221 cccataagcg ctaacttaga ggctcacgtg ctctttgccg ctgaagaacc cttttgcgtg
     5281 gcggctctgc gagccgatat cgcaatgctt gccactgcgc aatggcatcg acaagtctga
     5341 ggacgggtgt cacgcaacgg caaagcagtt caagcatgag cgaggtttca cgccacgggc
     5401 agtgctctcg cggacgagtc gaaccatgaa aattcgtatc cccaggggga aacgcgctcg
     5461 gagtgagcga tcaggcgagc aattaacagg gccaccagca gctttccctg cagccaactt
     5521 cgcgccgccc gcgcatcgtg cttcttcagg tggcccagct ggatgagcga cttcagccgc
     5581 ttgaattcca gctcgatctg ccagcgcagc cgatacagcg tcatgacttg ttcggcgctg
     5641 atatcgggcc ccagcgtggt gaagatcagc acgtactcgg ccgcctccag ggtctgaggc
     5701 tgcagttgcc ggcgcccgcg catgctctcg cggcgcaccc gatcacgccc catcgcagcg
     5761 gccgcagcgc tcttgcgcac cgcgcacagc cgccccacga tctcctgcgc gcgctggctc
     5821 ccacgctcgg ccttgacctt caggcaggcc ggccagctgc ccgcctgccc cacctgcagc
     5881 cctcgcacct gctgcagcac atccagcggc ttgcccgtgc tcgggtgcgt cagcgacagg
     5941 gtcaccaggt tcatgcgcac gatcacatcg ccgccatggc gcctgacatg ggcaattccg
     6001 ccggggtgcg catagccgcg gtcagccacg aagatgtcac ccacgtgcac ctggaagcgc
     6061 ttgagggttt ccccgtcgtc gcaagggccc aggtgcacct cctggcactg caggctcggc
     6121 agcccaatgg cgtagtgcag ccgccactgc gagccggtca cgccaggctc gcgcacgacg
     6181 ctgccatcca caacgcgcag ctgtcgcccg cccagcagcc cttgcgcccc ttcatcgggc
     6241 tgcccggcgc gcagctcgtg cgccatccac tcgaaccatg gtccacacga tcgcagcc
//
LOCUS       DASWIY010000429         3755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_669219, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000429 DASWIY010000000
VERSION     DASWIY010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669219"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1151
                     /locus_tag="VGA59_15260"
     CDS             246..1151
                     /locus_tag="VGA59_15260"
                     /inference="COORDINATES: protein motif:HMM:NF025596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HEX9721076.1"
                     /translation="MHQTLSYRHRVITDEDLVFIRKLIADHPDASRRNLSERLCEAWN
                     WVQANGALRAMVCRGLMLMLHRQGLIELPPVRRVNRNPFLERSAPAAVTVDEAPLGTS
                     LAELGTLEVRQVRRTPDEAVFNALMQQHHYLGYKQPVGEHLKYMVFAQGRPIACMGWS
                     SAPRHLGSRDRFIGWTAPARLANIALLAYNTRFLILPWVSVPHLASHLLGRIARRLSG
                     DWQSVYGHPIYFLETFIDPQRFGGTCYRAANWTLLGVTTGRGKDAPTYEPNRSIKQVL
                     GYALIKDFRQRLSRLDSPVKLPIAA"
     gene            1148..2791
                     /locus_tag="VGA59_15265"
     CDS             1148..2791
                     /locus_tag="VGA59_15265"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX9721077.1"
                     /translation="MKPPEIVNATQAELDELLAKAKPTFSCEQYLLLERVFGTFVYVM
                     LSLQNAKTSIKRFQRMLFGGRTEHKRNVLGPAGVAPELHGQDGAGSGAEPAQVVVPSP
                     KPHPPGHGRNAAQAYSGAPIVECVCPDLEPGDRCPRCDKGKVYDSPPKSLVKVVGQAP
                     LGATVYRVQRLRCRLCDAIFTAPLPAAVAQLPKYDASCASMIAVLRFGNGMPHFRLEG
                     LQASLYIPLPDATQWEIVSKAVPSPRAVFEELIRQAAQAPLLHSDDTPMKVLSLMKER
                     ARAEADGVKPAALAINTSGIVAVLQNNQNEPRKVVLFFTGHAHAGKNMERVLAHRAAE
                     LAPAMQMCDALAANVAGEFTTIVANCLAHGRRQVADVAEQFPEAARYVIEALAEVYKH
                     DATCREKAYSPEQRLSFHQLHSQPIMTALLAWMAEQFDKRLVEPNSGLGRALNYLIKH
                     WSELTLFLHRVGAPLDNNLCEQALKRAIIHRKGSLFYKTVRGAQVGDIYMSLIHTCRL
                     CDVNPFDYLNALQQRAREVIDAPARWLPWNFREQLAGDP"
     gene            complement(2812..3171)
                     /gene="tnpB"
                     /locus_tag="VGA59_15270"
     CDS             complement(2812..3171)
                     /gene="tnpB"
                     /locus_tag="VGA59_15270"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX9721078.1"
                     /translation="MIQITPHMRILVAVEPIDFRAGIDGLVNNCRKLLQADPFSGALF
                     VFGNRARTALKVLVYDGQGFWMCHKRLSTGKFTWWPAGHEQGHTLQACELQVLLMAGN
                     PTQAHAAPAWRALSVAA"
     gene            complement(3168..3599)
                     /locus_tag="VGA59_15275"
     CDS             complement(3168..3599)
                     /locus_tag="VGA59_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721079.1"
                     /translation="MKVTGKAAQVKAGADALRQLKGRFATWRAGRKLGMRIPPQLWTA
                     AARMAAVHGACRVAGELNLDYDMLKQRVALDSGKVTPVELAPRFVELYAAPGPMPAHS
                     ASQPQCVVELANARGARMRVELNGQGLAGLSVLCSAFLGAP"
BASE COUNT          628 a         1290 c         1222 g          615 t
ORIGIN      
        1 ggcttgagag aagccagact ctccatcgtc gtctcaggac gatttccctc gtctttgctc
       61 aaggtcactt cgtgctggcg cagaacgttg gtctccgtgt cggtcaccgc catgctcgtg
      121 tccctccggc aaatctgcat gaactgattt gagtatcgtg gcagcgcagt ttgccacgaa
      181 ggggacgcga agaatttgct gcgcccccct tgcgttccgt cgcggtaact gctaccgtgc
      241 ggcgcatgca ccaaacgctg agctaccgcc accgcgtgat caccgatgag gacctcgtct
      301 tcatccgcaa gctgattgcc gaccacccgg acgcgagccg gcgcaacctg tccgagcgac
      361 tgtgcgaggc ctggaactgg gtgcaggcca atggcgcgct gcgcgccatg gtgtgccgag
      421 gcctgatgct gatgctgcac cgccagggcc tgatcgagtt accgccggtg cgccgggtca
      481 accgcaatcc gtttctggag cgcagcgcgc cggcggcggt cacggtggat gaggcgccac
      541 tgggcacgag cctggccgaa ctcgggacgc tggaggtccg acaggttcgg cgcacgcccg
      601 acgaggcggt gttcaacgcc ctgatgcagc agcaccacta cctgggctac aagcagccgg
      661 tgggcgagca cttgaagtac atggtgttcg cccaaggacg gccgatcgcg tgcatggggt
      721 ggagttcggc gccgcgtcac ctgggcagcc gcgaccggtt catcggctgg acggcgccgg
      781 cgcggttggc caacatcgct ctgctggcct acaacacgcg cttcctgatc ctgccgtggg
      841 tgagcgtgcc gcatctggcc tctcacctgc tcgggcgcat cgccaggcgg ctgtcgggcg
      901 attggcagtc ggtgtacggc cacccgatct acttcctgga gaccttcatc gatccgcagc
      961 gctttggcgg cacctgctac cgcgcggcga actggacgct gctgggcgtg acgacagggc
     1021 gcggcaagga cgcacccacg tatgagccga accgctcgat caagcaggtg ctgggctatg
     1081 cgctgatcaa ggacttccgc cagcggctgt cgcggctgga ctcgccggtg aagcttccca
     1141 tcgctgcatg aagccgcccg agatcgtcaa cgccacgcag gccgagctcg acgaactcct
     1201 ggccaaggcc aagccgacct tctcctgcga gcaatacctc ttgctcgagc gagtgttcgg
     1261 caccttcgtg tacgtcatgc tgtcgctgca aaacgcgaag acgtcgatca agcgcttcca
     1321 gcgcatgctg tttggcggtc gcacggagca caagcgcaac gtgctggggc ccgctggcgt
     1381 ggcgcccgag ctgcacgggc aggacggcgc cggctcaggg gccgagccag cgcaggtggt
     1441 ggtgcccagc cccaagccac acccgcccgg gcacggccgc aacgcggcgc aggcctacag
     1501 cggtgccccc atcgtcgagt gcgtgtgccc cgacctggag ccgggtgacc gctgcccgcg
     1561 gtgcgacaag ggcaaggtct acgactcgcc cccgaagtca ctggtcaagg tcgtaggcca
     1621 ggcgccgctg ggcgcgacgg tctacagagt gcagcggctg cgctgccggc tgtgcgatgc
     1681 gatcttcacc gcgccgctgc cggcggcggt ggcgcaactg cccaagtacg acgcgagctg
     1741 cgccagcatg atcgccgtcc tgcgctttgg caacggcatg ccgcacttcc gcctcgaagg
     1801 cctgcaggcc tcgctgtaca tcccgctgcc cgatgcgacg cagtgggaga tcgtctccaa
     1861 ggccgtgccg tcaccgcgcg cggtgttcga ggagctgatc cgccaggccg cccaggcccc
     1921 gctgctgcac agcgacgaca cgccgatgaa ggtgctcagc ctgatgaagg aacgcgcccg
     1981 tgccgaggcc gacggcgtca agcccgcggc cttggcgatc aacacctcgg gcatcgtggc
     2041 cgtcctgcag aacaaccaga acgagccgcg caaggtggtg ctgttcttca ccggccacgc
     2101 ccacgcgggc aagaacatgg agcgcgtcct ggcccaccgg gccgcagagc tggccccggc
     2161 catgcagatg tgcgacgcgc tggccgccaa cgtcgcgggc gagttcacca ccatcgtggc
     2221 gaactgcctg gcccatggac ggcgccaggt ggccgacgtt gccgaacaat tccccgaggc
     2281 ggcgcgctac gtcatcgagg ctctggccga ggtctataaa cacgacgcaa cctgccgcga
     2341 gaaagcctat tcgcctgagc agcgcctgtc cttccaccag ctgcacagcc agcccatcat
     2401 gacggcgctg cttgcctgga tggccgagca attcgacaag cgcctggtcg agcccaactc
     2461 gggcctgggc cgcgcgctga actacctgat aaagcactgg agcgagctca cgctgttcct
     2521 gcacagggtc ggcgcgcccc tggacaacaa cctgtgcgag caggccctca agcgcgcgat
     2581 catccatcgc aagggctccc tgttctacaa gacggtgcgg ggcgcgcagg tcggcgacat
     2641 ctacatgagt ttgatccaca cctgccggct gtgcgatgtc aacccgttcg actatctcaa
     2701 cgcgcttcag cagcgagctc gggaggtgat cgacgcgccg gcgcggtggc tgccgtggaa
     2761 cttccgcgag cagctcgccg gtgatccgtg agttcagtcg tgagctgaag ttcaggccgc
     2821 gacactgagc gcgcgccacg cgggggcggc gtgtgcctgg gtggggttgc ccgccatcag
     2881 cagcacctgc agctcgcagg cctgcagggt gtggccttgc tcgtggccgg cgggccacca
     2941 cgtgaatttg cccgtggaca gccgcttgtg gcacatccag aaaccctggc cgtcgtacac
     3001 caggaccttc agggccgttc gggctcgatt gccgaagacg aacagcgccc cgctgaaggg
     3061 gtcggcctgc agcagcttcc tgcagttgtt caccagcccg tcgatgccgg cgcggaagtc
     3121 aatcggctcc accgccacca gaatgcgcat gtgcggcgtg atctggatca tggcgccccc
     3181 aggaaggcac tgcacagcac ggacaggccc gccagcccct ggccgttaag ctccacgcgc
     3241 atccttgcgc cgcgggcgtt ggctagttcg accacgcact gcggctgaga agcgctgtgc
     3301 gccggcatcg gcccgggcgc ggcgtacagc tcaacgaatc ggggcgccag ttcgactgga
     3361 gtgaccttgc cgctgtccag cgcgactcgc tgcttgagca tgtcgtagtc gaggttcagt
     3421 tcacccgcga cccggcaggc cccatgcacg gcggccatcc gggccgctgc ggtccacaac
     3481 tgcggtggga tccgcatccc cagcttgcgc cccgcgcgcc acgtcgcaaa ccgccccttc
     3541 aactgcctga gcgcgtccgc gcccgccttc acctgcgccg ccttgcctgt caccttcatc
     3601 gccgctctcc atccagggtt gatatggcag cagaagataa tcagttcaca tcgtcagttc
     3661 atgcaggttt gccggaagga cactgaactt caagcgccac acagcgcttg cagaggccat
     3721 ggctttcgcc gagcggtgcg cgcgctgctg gaagg
//
LOCUS       DASWIY010000430        11300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1800501, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000430 DASWIY010000000
VERSION     DASWIY010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11300
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800501"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..92
                     /locus_tag="VGA59_15280"
     CDS             <1..92
                     /locus_tag="VGA59_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9721080.1"
                     /translation="AHAVAFLASPRARYVTGQEIHVNGGMYMS"
     gene            194..433
                     /gene="acpP"
                     /locus_tag="VGA59_15285"
     CDS             194..433
                     /gene="acpP"
                     /locus_tag="VGA59_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9721081.1"
                     /translation="MSDIEARVKKIIAEQLGVEESQVTSEKAFVADLGADSLDTVELV
                     MALEDEFGIEIPDEDAEKITTVQNAIDYANAHQKA"
     gene            441..1688
                     /gene="fabF"
                     /locus_tag="VGA59_15290"
     CDS             441..1688
                     /gene="fabF"
                     /locus_tag="VGA59_15290"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX9721082.1"
                     /translation="MSRRRVVVTGLGCISPVGNTVAESWANLLAGKPGIDLITRFDAG
                     SFACKIAGEVKGFNIGDYIPEKEARHMDRFIHLGLAAACQAVADSGLPTGDALDGDQA
                     TRIGCNIGSGIGGLPLIEDMHAELVARGARRISPFFVPASIINMISGHVSIKYGFKGP
                     NIAVVTACTTGLHAVGLSARMIESGDADVMVAGGSESCVSPLGIGGFSAARALSTRND
                     DPGTASRPWDKDRDGFVLGEGAGVLVLEEYEYAKARGARIYAEVAGFGMSADAFHMTA
                     PDVDGPRRSMVNALRNAGINADQVNYLNAHGTSTPLGDLNETNAVKAAFGDAARNLVV
                     NSTKSMTGHLLGGAGGIESVFTVLAVHEQKSPPTINIFNQDPECDLDYCANTARDMRI
                     DIAMKNNFGFGGTNGTLVFKRID"
     gene            1730..2191
                     /locus_tag="VGA59_15295"
     CDS             1730..2191
                     /locus_tag="VGA59_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721083.1"
                     /translation="MHSAPSVSYPLGRSRFAAALLLMAMLLGGAVTALWGFQSPSPGW
                     RIGGACLVLAATGAFAAWQWWHCARGALAWDGQNWTWSGDRVAQSGGLLEVSLDLQHW
                     LLLRWTSGGESHWFWLDRASRAERWDDLRRAVYSRARPEALPDARRPAARP"
     gene            2188..2823
                     /gene="rpoE"
                     /locus_tag="VGA59_15300"
     CDS             2188..2823
                     /gene="rpoE"
                     /locus_tag="VGA59_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEX9721084.1"
                     /translation="MTKTPHAAPATPGDTDLMLVERTVAGDQKAFELLVLKYQRRIER
                     LIGRMVRDTDLVEDIAQETFIRAYRALSQFRGEAQFYTWLYRIAVNTAKKALGDLKRD
                     PLVSENALRAGNEEDETSAVENELTTAETPETVLAAKEIAAAVNSAMEALPEELRQAV
                     TLREIEGLSYEEIAEVMNCPIGTVRSRIFRARESISVKIKPLLENQSGKRW"
     gene            2848..3438
                     /locus_tag="VGA59_15305"
     CDS             2848..3438
                     /locus_tag="VGA59_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEX9721085.1"
                     /translation="MDRMQTQELISAMADGQLRGEAFARGVELAAGDPVALEAWHTYH
                     LIGDVLRSGELTPATMHTAFLGRLQARLRQEQRLTAARNAPEARPIVEPVAAHRVAVN
                     DDSFRWKLVAGFASVAAMAAIGWTVSGAVAGKVEQAQLAAAQRGTVLAGGERGVMIRD
                     PRLDELLAAHRQLGGASALQMPAGFLRNATFEGPTR"
     gene            3484..4506
                     /locus_tag="VGA59_15310"
     CDS             3484..4506
                     /locus_tag="VGA59_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEX9721086.1"
                     /translation="MARGIAALWAVCMLGGAQAQAAAPAPAIEQAVPAQGKYPERNVS
                     EWLMRMHEASRGRSYVGTFVVSSSGGGMSSARIWHVCDGQQQMERVESLTGAPRTTIR
                     RNEEVLTFLPGSRVVRIEKRESLGLFPDLLKSSESSIPDFYAARQVGRDRVAGFETEV
                     VELAPKDNLRFGYRIWSERKSGLVVKLQTMDAEGNVLEQAAFSELQLDARVRIGKLSR
                     MMAVPEGWRVEKAEAVKTDAAAEGWQLRSAVAGFKPISCYKRPAAGSSAPEGTLQWIF
                     SDGLAAVSLFVEAYDRHRHVQEGLFSSGATQTLTRRVQDWWLTAVGEVPPQTLKAFAL
                     SLERRK"
     gene            4606..6090
                     /locus_tag="VGA59_15315"
     CDS             4606..6090
                     /locus_tag="VGA59_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX9721087.1"
                     /translation="MLTIDWKKVRGYAVVIALAISGTVALMPAKPVVAQARFLPDFTD
                     LVEQVGPSVVNIRTVERARASGSGSAPGGMDEEMQEFFRRFFGQPMPGVPRQGPRPNR
                     PPQDEEAQPRGVGSGFILTQDGFVMTNAHVVDGAEEVIVTLPDKREFKARVVGTPDKR
                     TDVAVIKIEAHGLPAVRIGDITRLKVGEWVMAIGSPFGLENTVTAGIVSAKQRDTGDY
                     LPFIQTDVAINPGNSGGPLINMRGEVVGINSQIYSRSGGFMGISFAIPIDEAIRVAEQ
                     LRSPAGRVSRGRIGVQIDQVTKDVAESIGLGKPQGALVRSVESGSPADKAGVEAGDII
                     VRFDGKVIERATDLPRLVGNIKPGTRSTMTVFRRGGSKDLGVTIAEFEPEKPVRRTAE
                     KEDKAKGSPAAILVGLSVSELTDAQKKELKLKGGVKVDAVAEGAARAGIREGDIIVAI
                     SNTEIAGVKEFDAVVAKVDKTKPIPVLLRRGELATYALIRPARG"
     gene            6601..8412
                     /gene="lepA"
                     /locus_tag="VGA59_15320"
     CDS             6601..8412
                     /gene="lepA"
                     /locus_tag="VGA59_15320"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX9721088.1"
                     /translation="MNHIRNFSIIAHIDHGKSTLADRLIQRCGGLSDREMEEQVLDSM
                     DLERERGITIKAQTASLRYTARDGQVYDLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTALDLGVEVVPVLNKMDLPQADPESAKSEIEDVIGIDAEHAIP
                     CSAKTGMGIDDILEAVVARIPAPRGNPQGPLRAMIIDSWFDSYVGVVMLVRVVDGRVA
                     KGERIKLMATGATYNADNLGVFTPANEPRESLEAGQVGYIIAGIKELQAAKVGDTVTL
                     IKPGTGGAAFTASEPLPGFKEIQPQVFAGLYPTEANQYDSLRDALEKLKLNDASLHYE
                     PEVSQALGFGFRCGFLGLLHMEIVQQRLEREFDQDLITTAPSVVYQVLKANGEMLMVE
                     NPSRIPDQGQVQEIREPIVTVHLYMPQEYVGPVMTLANQKRGLQLNMAYHGKQVMLTY
                     EMPLGEIVLDFFDKLKSVSRGYASMDYAFKEFRASDVVKIDILLNGEKVDALSVIVHR
                     SQSQYRGRAVVAKMRQIISRQMFDVAIQAAIGANIIARETIKALRKNVLAKCYGGDIT
                     RKRKLLEKQKAGKKRMKQIGSVEVPQEAFLAILQVEE"
     gene            8409..9377
                     /gene="lepB"
                     /locus_tag="VGA59_15325"
     CDS             8409..9377
                     /gene="lepB"
                     /locus_tag="VGA59_15325"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9721089.1"
                     /translation="MMQALTAAVLAAFAGYAGAWYLGMVDGNFALLLFLATVVTGLYW
                     LAERLYFLPQRQLAATRLEAHAARRHADLSAKGISQVDGDVAQARQRLLMQPWWLDWT
                     AGLFPVILAVFVLRSFLFEPFKIPSGSMIPTLLVGDLILVNKFTYGVRLPVLNTRLTE
                     GSAPRRGDVMVFRYPPKPSLDYIKRVVGVPGDEVAYLNKQLTINGQPVPKKALPDFFD
                     EDAMLYFKQFEEVLGEGRHRILNDDNRPAFIPGVEDFQFKQNCRYSVEGVVCKVPQGH
                     FFMMGDNRDNSLDSRYWGFVPDRNIVGRAFFIWMNFGSFSRIGRFE"
     gene            9409..9762
                     /locus_tag="VGA59_15330"
     CDS             9409..9762
                     /locus_tag="VGA59_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HEX9721090.1"
                     /translation="MKPYFKSRQLGISFIGLLFVVGVLASLAVLGAQAFPTVVEYQAI
                     LKATQKASEGNTVAEVRQIFERATAIDDIKSVTPKDLDISKNGDKVVVKFAYTREIHM
                     FGPAWLLLKYAGQSK"
     gene            9777..10460
                     /gene="rnc"
                     /locus_tag="VGA59_15335"
     CDS             9777..10460
                     /gene="rnc"
                     /locus_tag="VGA59_15335"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX9721091.1"
                     /translation="MDGQLVALQARLQHQFADRQLLARALTHRSFSADHNERLEFLGD
                     SVLNLAVAALLYERLKALPEGDLSRVRANLVKQETLHQLAIGLGLPELVRLGEGEMRS
                     GGSRRPSILADALEALIGAVFLDAGYPAGEALVRRLFDKVEINPTMAATAKDPKTELQ
                     EWLQGRKMKLPIYRVAAKLGAAHKQTFDVECEIAELGIVERGIGASRRAGEQAAAAAM
                     LLALKAREK"
     gene            10460..>11300
                     /gene="era"
                     /locus_tag="VGA59_15340"
     CDS             10460..>11300
                     /gene="era"
                     /locus_tag="VGA59_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX9721092.1"
                     /translation="MEAASLRCGSIAIVGKPNVGKSTLLNALVGQKISITSRKAQTTR
                     HRITGIRTIGATQFVFVDTPGFQTRHATALNRSLNKTVMGAVGDVDLVLFVVEAGKFT
                     LADAKVLSLLKPGVPALLVANKLDQIHRRGDLAPWLRDMQQRHDFNEFVPMSAKNPKD
                     VERLFAICEKYLPAQAWWYGPDELTDRTEKFLAAETVREKLFRFTGDELPYTSTVVVD
                     KFEEEPSPKHGRMVRIAATIVVEREGHKAMVIGDKGERLKRIGTEARQELEKLMDCKV
                     FLEI"
BASE COUNT         2109 a         3548 c         3716 g         1927 t
ORIGIN      
        1 tcgcccatgc ggtggcattc ctcgcatcgc ccagggcccg gtatgtgacg ggacaggaaa
       61 tccacgtcaa cggcggcatg tacatgtcct gattcagaat aggccggttt atccgcccgg
      121 cggctgctgg cagggaaaag acccggccgg cagtctaaaa tcgcggattc attcacaacc
      181 ctctgaggga accatgagcg atatcgaagc acgtgtcaaa aaaatcatcg ccgagcaact
      241 cggcgtggaa gagtcccagg tcaccagcga gaaggccttc gtggccgacc tgggtgccga
      301 ctcgctggac acggtggaac tggtgatggc gctcgaggac gagttcggca tcgagatccc
      361 cgacgaggat gccgagaaga tcaccacggt gcaaaacgcg atcgactacg cgaacgccca
      421 ccagaaggcc tgatccgaaa atgagccgtc gccgcgtcgt cgtgaccggc ctgggttgca
      481 tcagccccgt gggcaacacg gtggccgaat cctgggccaa cctgcttgcc ggcaagcccg
      541 gcattgacct gatcacgcgg ttcgatgccg gcagtttcgc ctgcaagatc gcgggcgagg
      601 tcaagggttt caacatcggg gactacattc ccgaaaagga agcccggcac atggaccgtt
      661 tcatccacct gggccttgcc gccgcctgcc aggccgtggc cgacagcggg ctgcccacgg
      721 gcgacgcgct ggacggcgac caggccaccc gcatcggctg caacatcggc tcgggcatcg
      781 gcggtctgcc gctgatcgag gacatgcacg cggagctggt cgcgcgcggt gcgcgccgca
      841 tctctccgtt cttcgtgccg gcgtcgatca tcaacatgat ctccggccat gtctcgatca
      901 agtacggctt caagggtccc aacatcgcgg tggtcaccgc ctgcacgacc ggcctgcacg
      961 ccgtcggact gtcggcgcgg atgatcgaaa gcggcgacgc cgacgtcatg gtcgccggcg
     1021 gctccgaatc ctgcgtgtcg cccctgggca tcggcggctt ttcggccgcg cgcgccctgt
     1081 ccacgcgcaa cgacgacccc gggacggctt cacggccctg ggacaaggac cgggacggct
     1141 ttgtgctggg cgaaggcgcc ggtgtgctgg tgctggaaga gtacgaatac gccaaggccc
     1201 gcggcgccag gatctatgcc gaagtggccg ggttcggcat gagcgccgat gccttccaca
     1261 tgaccgcgcc ggacgttgac ggaccgcgtc gctcgatggt caatgcgctg cgaaatgccg
     1321 gcatcaacgc cgaccaggtg aattacctca acgcccatgg cacgtccacg ccgctgggcg
     1381 acctcaacga aaccaacgcc gtcaaggccg cgttcgggga tgcggccagg aacctggtcg
     1441 tcaactcgac caagtcgatg acgggccacc tgctgggcgg cgccggcggc atcgaatcgg
     1501 tgttcaccgt gctggccgtg cacgagcaaa agagcccgcc cacgatcaac atcttcaatc
     1561 aggacccgga atgcgacctg gattactgcg ccaatacggc gcgggacatg cggatcgaca
     1621 tcgcgatgaa aaacaacttc ggtttcggcg gtaccaatgg cacgctcgtt ttcaagcgca
     1681 tcgactgagc ctccgataaa gttcgcgcgt acgtgaggcc gggccttcca tgcacagcgc
     1741 cccatcggtg agctatccgc tggggcgctc gcgtttcgcg gccgccctgc tgctgatggc
     1801 catgctgctg gggggcgccg tgaccgcgtt atggggcttc cagtccccat cgcccggctg
     1861 gcgcatcggt ggggcttgtc tcgtgcttgc cgcgacggga gctttcgcag cgtggcaatg
     1921 gtggcattgc gcgcgcggcg ccctggcatg ggacgggcag aactggacct ggtcgggcga
     1981 ccgggtcgcg caatccggcg gcttgctcga ggtcagcctc gacctgcagc attggctgct
     2041 gttgcgctgg acttcaggcg gcgagtcaca ctggttctgg ctggatcgtg cgtcccgcgc
     2101 cgaacgctgg gatgatttgc gtcgtgcggt atattcccgc gccagacccg aagcgctgcc
     2161 cgacgcccgc cgacccgcag cgagaccatg accaagacgc ctcacgccgc ccctgccacg
     2221 cctggcgaca ccgacctgat gctggtggag cgcactgtgg cgggcgacca gaaggcgttt
     2281 gagctgctgg tgctgaagta ccagcgccgc atcgaacggc tgatcggccg gatggtgcgc
     2341 gacaccgacc tggtcgagga catagcgcag gaaactttca tccgcgccta ccgcgcgctt
     2401 tcccagttcc ggggcgaagc ccagttctac acctggcttt accggatcgc ggtgaatacg
     2461 gcgaagaagg ccctgggcga cctcaagcgc gaccccctgg tttcggaaaa cgccctgcgc
     2521 gcaggcaacg aggaagatga aacttccgcc gtggagaacg aactaaccac cgcggaaacg
     2581 ccagaaacgg tgctggccgc caaggaaatc gccgccgcgg tcaactcggc catggaagcc
     2641 ctgcccgaag aattgcgcca ggcagtgacc ctgcgcgaga tcgaggggct gagctacgag
     2701 gaaatcgcgg aagtgatgaa ttgcccgatt ggtaccgtgc ggtcccgcat tttccgggct
     2761 cgcgagtcga tctcggtcaa gatcaagccc ctgctggaaa accagtcggg caagcgttgg
     2821 tgaagcccga tgcggcgagg tgacgttatg gacaggatgc agacgcagga actcatttcg
     2881 gcaatggcgg acgggcagtt gcgcggggag gccttcgcgc gcggggtgga gcttgcggcc
     2941 ggcgatcctg tggccctgga ggcctggcat acctatcacc tcattggcga cgtgttgcgc
     3001 tcgggcgaac tcactcccgc aaccatgcac acggcctttc tcgggcgcct tcaggcccgg
     3061 ctacggcagg agcagcgcct cacggccgca cgaaacgcgc ccgaggcgag gccgatcgtg
     3121 gagccggtcg ccgcgcaccg cgtcgcggtc aacgatgaca gctttcgctg gaagctggtg
     3181 gccggattcg cgtccgtcgc cgcgatggcc gcgatcggct ggaccgtgtc gggcgccgtc
     3241 gccgggaagg tggaacaggc ccaattggcc gctgcccagc gcgggaccgt gctcgccggc
     3301 ggcgagcgcg gcgtcatgat ccgggatccg cggctggacg agttgctggc cgcgcaccgc
     3361 cagctcggcg gcgcttcggc cttgcagatg ccggccggtt tccttcgcaa tgccaccttc
     3421 gagggaccta cccgctgagg cggccaacgc atgggatacc agttcacgcc gagaagaata
     3481 atcgtggccc gcggcatcgc cgccctctgg gcggtttgca tgcttggggg tgcgcaagcc
     3541 caggcggcgg cgccggctcc cgcgatcgag caggccgtgc cggcccaggg caagtacccg
     3601 gagcgcaacg tcagcgaatg gctgatgcgc atgcacgagg cttcgcgcgg gcgcagctat
     3661 gtcggcacct tcgtcgtgtc ttcaagcggc ggcggcatgt ccagcgcgcg gatctggcat
     3721 gtctgcgacg gccagcagca gatggagcgg gtggaatcgt tgaccggtgc gccgcgtacg
     3781 accatccgcc gcaatgaaga ggtcctcacc ttcctgccgg ggagccgtgt ggtccgcatc
     3841 gaaaaacggg agtcgctggg cctgttcccg gacctcctca agtcaagcga aagctccatc
     3901 cccgatttct acgccgcgcg ccaagtgggc cgtgaccggg tcgcgggctt cgaaaccgaa
     3961 gtggtcgagc tcgccccgaa ggacaacctg cggttcggct accgcatctg gagcgaacgc
     4021 aaaagcggat tggtcgtcaa gctgcaaacc atggacgcag agggcaacgt gctggagcag
     4081 gcggctttct ccgaactcca gctcgacgca cgcgtgcgca tcggcaagct cagccgcatg
     4141 atggcggtgc cggaaggctg gcgtgtcgaa aaggccgagg ccgtcaagac cgacgccgca
     4201 gccgaaggct ggcaactgcg gtcggccgtg gcggggttca aaccgatcag ctgctacaag
     4261 cggccggcgg ccggctcttc ggcgcccgag ggcacgctgc aatggatctt ttccgatggc
     4321 ctggcggcgg tttcgctgtt cgtcgaagct tacgaccggc acaggcacgt ccaggaaggc
     4381 ctgttctcca gcggcgccac ccagacgctc acgcgccgtg tccaggattg gtggctcacg
     4441 gcggtgggcg aagtgccgcc ccagaccctg aaggccttcg cgctgagcct tgagcgccgc
     4501 aaataaggcc ccagattccc cggaatatgc gatggttcga gcttgccggc gcgaaccatt
     4561 tgcccgcttt ttgttctcag ctttatctct gattgaaagg tccacatgct aacaatcgat
     4621 tggaagaaag tgcgcggcta cgccgtggtg atcgcgctgg cgatctccgg caccgtggcg
     4681 ctgatgcccg ccaagccggt cgtggcgcag gccaggttct tgccggattt cacggacctg
     4741 gtggaacagg tcggcccgtc ggtggtgaac atccgcactg tcgaacgtgc ccgcgccagc
     4801 ggttccggtt ccgcgcccgg tggcatggac gaggagatgc aggagttctt ccggcggttc
     4861 ttcggccagc ccatgcccgg cgtgccgcgt cagggcccgc gcccgaaccg tccgccgcag
     4921 gacgaggagg cgcagccccg gggagtgggg tccggtttca tcctgacgca ggacggcttc
     4981 gtcatgacca acgcccacgt ggtcgacggc gcggaagaag tcattgtcac cttgcccgac
     5041 aagcgcgagt tcaaggccag ggttgtcggc actcccgaca agcgcaccga cgtggctgtc
     5101 atcaagatcg aagcgcacgg gctgcccgcg gtgaggatcg gcgacatcac gcgcctcaag
     5161 gtcggcgaat gggtgatggc catcggttct ccgttcggcc tggagaacac cgtgactgcg
     5221 ggaatcgtga gcgcgaagca gcgcgacacc ggcgactacc tgcccttcat ccagaccgac
     5281 gtggcgatca accccggcaa ttcgggtggc ccgctgatca atatgcgcgg cgaggtggtg
     5341 ggcatcaaca gccagatcta ctcccgctcg ggcggcttca tgggaatctc gttcgcgatt
     5401 ccgatcgatg aggcgattcg cgtggcggag cagctgcgtt cgccggccgg gcgtgtctcg
     5461 cgcggccgta tcggcgtgca gatcgaccag gtgaccaagg acgtggccga atcgatcgga
     5521 ctgggcaagc cgcaaggcgc cctggtgcgc agcgtggaaa gcggttcgcc cgccgacaag
     5581 gccggcgtcg aggccgggga catcatcgtg cgtttcgacg gcaaggtgat cgagagggca
     5641 accgacctgc cgcgcctggt tggcaacatc aagcccggca cgcgcagcac gatgacggtg
     5701 ttccgccgcg gcggttccaa ggacctgggt gtcacgatcg ccgagttcga gcccgagaag
     5761 ccggtgcgcc gcaccgccga gaaggaggac aaggcgaagg gatcgccggc cgcgatattg
     5821 gtgggcttgt cagtgagcga actcaccgat gcgcagaaga aggagctcaa gctcaaaggc
     5881 ggcgtgaagg tggacgcggt cgccgaaggc gccgcgcgcg cgggcattcg cgaaggcgac
     5941 atcatcgtgg ccatcagcaa taccgaaatc gcgggcgtca aggagttcga cgcggtggtg
     6001 gcgaaggtcg acaagaccaa gcccatcccg gtgctgttgc ggcgcgggga attggcgacc
     6061 tacgccctga tccggcctgc ccgcggctag gccggcggct tggcgtgcaa ggccccagcc
     6121 cgtggtcggg tttggggctt tcctgtgtgc agtgaccaaa aaaaaacggg cgctggaaat
     6181 atttttctgt ccccggcgtc cgcccttaag ttggtggaca ctgacttttt ggacgggcta
     6241 attacatttt tggatagaat ctgggcacct catcggcgat tagtggcata tcgaaaagtg
     6301 cctgaaccac catacgggat gaattcccat gctgcacagc cgatccacag atcgcccaca
     6361 acttgtccca aagcttgccc cccaaatatg ttgataagtt gtgaataaca cgcatgttgc
     6421 tggcctgcaa cggccctcgt cgaccctcgg ttcggctgta cgccctgggc tttttgccta
     6481 caatgaagct ccaactatcg cagttgccat agattgttgg tttagggcgc gtcaccagtc
     6541 gacgcgccct tttttctttt ccggcgccgt ttgaattcaa tcacttaagt cttcccgttg
     6601 atgaatcaca tcagaaactt ttcgatcatc gcgcacatcg atcacggcaa atccacgctc
     6661 gcggaccgct tgatccaacg ttgcggcggc ttgtcggatc gcgagatgga agagcaggtg
     6721 ctcgactcga tggaccttga aagggaacgt gggataacca tcaaggcgca gaccgcttcg
     6781 ctgcgctaca cggcgcgcga cgggcaggtc tacgacctca atctgatcga cacacccggc
     6841 catgtggact tctcgtacga ggtctcccgt tcattgtccg cgtgcgaagg cgcgctgctg
     6901 gtcgtcgatg ccagccaggg cgtggaagcg cagaccgtgg ccaactgcta caccgcgctg
     6961 gacctcggcg tggaggtcgt gccggtactc aacaagatgg acctgccgca ggccgacccc
     7021 gagagcgcca agtcggaaat cgaagacgtg atcggcatcg atgcggagca tgcgatcccc
     7081 tgctcggcca agaccggcat gggcatcgac gacatcctcg aggcggtcgt ggcccgcatc
     7141 ccggcgccgc gcggcaaccc gcaaggaccc ctgcgcgcga tgatcatcga cagctggttc
     7201 gacagctacg tcggtgtcgt catgctggtg cgggtggtgg acgggcgcgt ggccaagggc
     7261 gaacggatca agctcatggc cacgggcgcg acctacaacg ccgacaacct gggggtcttc
     7321 accccggcca acgagccgcg cgagtccctc gaagcggggc aggtgggcta catcatcgcc
     7381 ggcatcaagg agctgcaggc cgccaaggtc ggggacaccg tcacgctgat caagccgggc
     7441 accggtggtg cggccttcac ggccagcgag ccgctgccgg gcttcaagga aatccagccc
     7501 caggttttcg cggggctgta cccgaccgaa gcgaatcagt acgactcctt gcgcgacgcg
     7561 ctggaaaaac tcaagctcaa cgacgcctcg ctgcattacg agcccgaagt cagccaggcc
     7621 ctgggcttcg gcttccgctg cggcttcctc ggcttgctgc acatggagat cgtgcagcag
     7681 cggctggagc gcgaattcga ccaggacctg atcaccaccg cgccgagcgt ggtgtaccag
     7741 gtgctcaagg ccaacggcga aatgctgatg gtcgagaacc cgtccaggat tccggaccag
     7801 ggccaggtcc aggaaatccg agagcccatt gtcacggtcc atctctatat gccgcaggaa
     7861 tacgtgggcc cggtgatgac cctggccaac cagaagcgcg ggctgcagtt gaacatggcc
     7921 taccatggca agcaggtcat gctgacttac gagatgccgc tgggcgagat cgtgctggac
     7981 ttcttcgaca agctcaagtc cgtgagccgc ggctacgctt ccatggacta cgcgttcaag
     8041 gagttccggg cttccgacgt ggtcaagatc gacatcctgc tcaacggcga gaaggtcgac
     8101 gctttgtccg tcatcgtcca ccgcagccag tcccaatacc gcggccgggc cgttgtcgcg
     8161 aagatgcgcc agatcatcag ccgccagatg ttcgacgtgg cgatccaggc ggccatcgga
     8221 gccaatatca tcgcgcgaga aaccatcaag gcgctgcgca agaacgtact ggcaaaatgc
     8281 tacggcggcg acatcacccg caagcgcaag ctccttgaaa agcagaaggc aggcaaaaaa
     8341 agaatgaagc agatcggttc ggtcgaagtc ccccaggaag cgtttctcgc catattgcag
     8401 gtggaggaat gatgcaggcg ttgacggccg cggtgctcgc ggcgttcgcc gggtatgccg
     8461 gcgcctggta cctgggcatg gtggatggca atttcgccct gctgctgttc ctggccaccg
     8521 tcgtcaccgg cctttactgg ctggccgagc ggctgtattt ccttccccag cggcagctgg
     8581 ccgctacccg gctggaagcg catgcggccc ggcgtcacgc ggacctttcg gccaagggca
     8641 tcagccaggt cgacggcgac gtggcgcagg cccggcagcg gctgctgatg cagccttggt
     8701 ggctggactg gaccgccggc ctgttcccgg tgatcctggc ggtgttcgtg ctgcgatcgt
     8761 tcctgttcga gcctttcaag atcccatccg gatcgatgat cccgaccctg ttggtgggag
     8821 acctcatcct ggtgaacaaa ttcacttacg gcgtccggct tccggtcctc aacaccaggc
     8881 tgaccgaggg cagcgcgccc cggcgcggcg acgtcatggt gtttcgctac cccccgaagc
     8941 ccagcctgga ctacatcaag cgggtggttg gcgtgccggg cgacgaggtt gcgtacctca
     9001 acaagcaact cacgatcaat ggccagccag tgccgaagaa agcgttgccg gatttcttcg
     9061 atgaggacgc gatgctgtac ttcaagcagt tcgaggaggt gctcggcgag gggcgccacc
     9121 gcatcctgaa cgacgacaac cggccggcgt tcatcccggg tgtcgaggac tttcagttca
     9181 agcagaattg ccgttacagt gtcgagggcg ttgtctgcaa ggtcccgcag gggcacttct
     9241 tcatgatggg cgacaaccgc gataattcgc tggattcgcg ctactggggt ttcgtgcccg
     9301 accgcaacat cgtgggcagg gcattcttca tctggatgaa tttcggcagt ttcagccgga
     9361 tcgggcggtt cgaataggtt cctgtttttt tgcttcgagg ggttagccat gaagccatat
     9421 ttcaagtcga gacaactcgg catttcgttc atcggcctgc tgttcgtggt gggcgtgctg
     9481 gcatcgctgg ccgtgctggg ggcacaggcc tttcccacgg tggtggaata ccaggcgatc
     9541 ctgaaggcga cccagaaagc ctcggaaggc aacacggtgg ccgaagtgcg gcagatcttc
     9601 gaacgggcga ccgctatcga cgacatcaag tccgtcactc ccaaggacct ggatatctcc
     9661 aagaacggcg acaaggtcgt ggtgaagttc gcctatacca gggagatcca catgttcggc
     9721 cctgcctggc tgctgctgaa atacgcggga cagtccaaat aagaaccaag gcaagcgtgg
     9781 atggccagct cgtcgcactg caggcacgct tgcagcacca gttcgccgac cggcagctgc
     9841 tggcgcgggc gctgacgcac cgcagctttt cggccgacca taacgagcgg ctggaattcc
     9901 tgggtgattc cgttctcaac ctcgcggttg ccgctctgct ctacgaacgg ctgaaggcac
     9961 tgcccgaagg cgacctgtcc cgtgtgcgcg ccaacctggt caagcaggaa accttgcacc
    10021 agctcgcgat cggcctgggc ctgccggagc tcgtgcggct gggcgaaggc gagatgcgtt
    10081 ccggcggcag ccggcgcccc tcgatcctgg ccgacgcgct ggaagcgctg atcggcgctg
    10141 tcttcctgga cgccggctac ccggccggcg aagcgctggt gcgccgcctg ttcgacaagg
    10201 tcgagatcaa tcccaccatg gccgcgacgg ccaaggaccc gaagaccgag ctgcaggagt
    10261 ggctgcaagg gcgcaaaatg aagctgccga tctaccgggt ggcggcgaaa ctgggcgccg
    10321 cccacaagca gacgttcgac gtggaatgcg agattgccga gctgggaatc gtggagcgcg
    10381 gcatcggcgc ctcgcggcgc gccggcgagc aggccgccgc cgcggccatg ctgctggcgc
    10441 tgaaggccag ggagaaatga tggaagcggc ttcccttcgc tgcggctcga tcgccatcgt
    10501 gggcaagccc aacgtgggca agtccacgtt gctcaatgcg ctggtgggcc agaagatcag
    10561 catcacttcg cgcaaggcgc agaccacgag gcaccgcatc accggcatcc gcaccattgg
    10621 cgcgacccaa ttcgtcttcg tcgatacgcc gggcttccag acccggcatg ccacagcgct
    10681 gaaccgctcg ctcaacaaga cagtgatggg ggcagtgggc gatgtcgacc tggtgctgtt
    10741 cgtggtggag gccggcaagt tcacgctggc ggacgccaag gtcctgtcgc tgctcaagcc
    10801 cggggtgccg gcgctgctgg tggcgaacaa gctcgaccag atccatcgtc gcggcgacct
    10861 tgcgccctgg ttgcgcgaca tgcagcagcg ccacgatttc aacgagttcg tgccgatgtc
    10921 cgccaagaac cccaaggacg tggaacggct gttcgcgatc tgcgagaaat acctgcccgc
    10981 gcaggcctgg tggtatggcc cggacgagct caccgaccgc acggaaaaat tcctggccgc
    11041 cgagaccgtg cgcgaaaagc tgttccggtt caccggcgac gagctgccct acacctccac
    11101 cgtggtggtc gacaagttcg aggaagagcc cagtccgaaa cacggccgca tggtcaggat
    11161 cgcggcgacc atcgtggtcg agcgcgaggg ccacaaggcc atggtgatcg gcgacaaggg
    11221 cgagcggctc aagcggatcg gcaccgaggc gcgccaggag ctggaaaagc tgatggattg
    11281 caaggtgttc ctggaaatct
//
LOCUS       DASWIY010000431         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2899753, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000431 DASWIY010000000
VERSION     DASWIY010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2899753"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="VGA59_15345"
     CDS             complement(<1..1501)
                     /locus_tag="VGA59_15345"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX9721093.1"
                     /translation="MQSTRSLKTWAGLLTASLIGLFSGPPAAAATAGYRTQSGQIYDA
                     AGQEIQLRGISHFGFNADILQPQYLWSMGWKEQIAQIKGLGFNAVRVPFVPDTLYVTT
                     TVDKLSWVDPNKNADLLGKTPLQVLDLWMAEADRQGLYILLDFHSVAKARQYPTWFVD
                     NPADFGITWNGQAYTEDHWVRDLKFVAQRYAALPHFIGIDPYNEPNGKVRWSAGDPNM
                     TEAKYYWKPAVEKAAAGILQSNPNLLIFVQGITANWDGVENSGVPMNWGENFQPQAYQ
                     PLNVAAGKLVLSPHTYGPDVYVKSSFSAANFPANLAADWDTLFGKFSTQVPVVVGEWG
                     GRYGQGGVGQADVTWQNAFVDYLLGKGIRSSFYWCYTPNSGDTGGILTDALEVREDKM
                     TLLRRHWGAAPATTTSEPAPTPEPAPATPGSLALSAASYSVLQSAGSVTIKVNRAGGS
                     TGAVSVRFETASGTARARTDYTARRGTLSWAAGDVAAKNVAISISNTTPF"
BASE COUNT          329 a          686 c          741 g          389 t
ORIGIN      
        1 tgaacggcgt ggtgttgctg atcgaaatcg cgacattctt tgccgcgacg tcgccggccg
       61 cccagctcag ggttccgcgc cgggccgtgt agtcggtccg ggccctggcg gtgccgctgg
      121 cggtctcgaa gcggacgctg accgcgccag tggagccccc ggcacggttg accttgatcg
      181 tcaccgaacc cgcactctgc agaacgctgt agctggcggc ggacaaggcc agcgaacccg
      241 gcgtcgcggg cgcgggctcg ggcgtgggcg ccggctcgga tgtcgtggtg gccggcgcgg
      301 ctccccagtg gcgccgcagc agcgtcatct tgtcttcgcg cacctcgagg gcgtcggtga
      361 ggatgccgcc ggtgtcgccg ctgttgggcg tgtagcacca gtagaagctg ctgcggatgc
      421 ccttgcccag caggtaatcg acgaaggcgt tctgccaggt gacgtcagcc tgaccgacac
      481 cgccctgccc gtagcgtccg ccccactcgc ccacgaccac cgggacctgg gtcgagaact
      541 tgccgaacag cgtgtcccag tccgccgcga ggttggccgg aaaattcgcg gcgctgaacg
      601 aggatttcac gtaaacgtcc ggcccatagg tatgcggcga cagcaccagc ttgcccgcgg
      661 cgacgttgag cggctgataa gcctgcggct ggaagttctc gccccagttc atcggcacgc
      721 cgctgttctc gacaccgtcc cagttggcgg tgatgccctg cacgaagatc agcaggttcg
      781 gattcgactg caggatgccg gccgcggctt tctcgaccgc cggcttccag tagtacttgg
      841 cttcggtcat gttggggtcg ccggcgctcc agcgcacctt gccgttcggc tcgttgtacg
      901 gatcgatgcc gatgaagtgc ggcagcgcgg catagcgctg cgcgacgaac ttgaggtcgc
      961 gcacccagtg gtcctcggtg taggcctggc cgttccaggt gatgccgaaa tccgccgggt
     1021 tgtcgacgaa ccacgtcggg tactggcgcg ccttcgccac gctgtggaaa tccagcagga
     1081 tgtagaggcc ctggcgatcg gcttccgcca tccacaggtc gagcacctgc agcggcgtct
     1141 tgccgaggag gtcggcgttc ttgttcggat cgacccagct cagcttgtcg accgtcgtgg
     1201 tgacgtacaa ggtgtccggc acgaacggca cgcgcaccgc gttgaagccc aggcccttga
     1261 tctgcgcgat ctgctccttc cagcccatcg accacaggta ctgcggctgc aggatgtcgg
     1321 cattgaagcc gaagtggctg atgccgcgca gctggatttc ctggccggcc gcatcgtaga
     1381 tctgtccgga ctgcgtacgg tagcccgccg ttgcggccgc tgcgggcgga ccgctgaaga
     1441 gaccgatcaa cgatgccgtc aacagcccgg cccacgtttt gagcgaacga gtggattgca
     1501 cgggaggact ctccggttgt gagacagctc ggggaattcg cgcggcgtgc tccactccgt
     1561 aggcacgcgc atggcgccag actaaaactg cgaaaccctg tctgtgcgct ctacctccct
     1621 taatgagggt ggtaggacac attgcaagag tggcgttgaa cgacggcctc aatcgtcgtc
     1681 tgtgcgctat cgcgcaatcc cggcacgggt cagcagcagc ccggcgcgga tttgtctatg
     1741 cggtttagca cgttctggcg ccgacgcgag aattattttt tgcgtctgtg cgctatccga
     1801 cgatcgccgg cggcgcgcgc acgcgcgcgc cacgccgcgg cgctgtgtgc ggcggcggac
     1861 ggaggggact gtaaccgctt gcgaatcagc gcagcgggtt gtggctcgcg accaggacgc
     1921 ggcatggccg gagcggcacg cgctccagcg atgggatcag gtcgactttg gtcgtagtcg
     1981 cggccgcggc cgcggaacgg tcgcgccgcg gcccgcgccg ggcccggcgc tagagcgctt
     2041 tcgatttatt cgattacaga ttgcctcagg gtagtggcag aggcggatcg cggggccgtt
     2101 ggcgcgaatg gctcgccgcg gtgctcctcg gtcgcgggcg gccaa
//
LOCUS       DASWIY010000432         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3265381, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000432 DASWIY010000000
VERSION     DASWIY010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3265381"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VGA59_15350"
     CDS             complement(<1..538)
                     /locus_tag="VGA59_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9721094.1"
                     /translation="MSAAGEHDPPRARTWMTGSARWWLGALLVLVLPPWLLPEFPITM
                     LNYIGLATLVTLGLVVMTGVAGIVSFGQQAFVGIAAYATAALTVLAGGSPWLGLAASL
                     LLVAACAALIGWVTLRLSGHYLSLATIAWGIAIYYLFGNLPLLGQYSGIDNVPAISVA
                     GWVFDSGRKSYLLIWIAAM"
     gene            complement(535..1581)
                     /locus_tag="VGA59_15355"
     CDS             complement(535..1581)
                     /locus_tag="VGA59_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9721095.1"
                     /translation="MNSDIALWLIQDGVTTGAIYALLALALLLVFAVTRIIFVPQGDF
                     ISYAGLTLGTLQLGVMPGTVYLLVGGGALIAVLDGYAAWREGAPRRLLRIVLVNVALP
                     VAVAALTAWAAPRKLDAVWLIALTMALVIPLGPMLYRLAYRPLADASVLVLFIISMAS
                     HYALSGLALAIFGGEGLRTNPISDAKFPLGVLQISAQSLWVLAATLLCMAALWAFFER
                     SLTGKALRATAMNRVGARLVGIPTATAGEIAFWLAAAIGALSGILISPIATIYYDTGL
                     MIGLKGFVGAILGGLASYPLAAVGALVVGLLEAYASFWASPLKETIVFGFLIPVLLWR
                     SLTSRHPVVEDEEE"
     gene            complement(1646..>2191)
                     /locus_tag="VGA59_15360"
     CDS             complement(1646..>2191)
                     /locus_tag="VGA59_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9721096.1"
                     /translation="QVLKVMAASPDAVIVGGSGSPGATPQIALSERGFKGPIYHNHGT
                     VNLPFIQTGKKAAEGAIAPTGALIVAEELPDNFPTKKVSLDFVKRYEAAFGPSSRNAF
                     AGYTYDGVMLLDAAAGVALKKAKPGTPEFREALRAALENVKNVVGTHGVYNLSPANHN
                     GLDERARVLVQVVNGEWRLMK"
BASE COUNT          410 a          754 c          725 g          302 t
ORIGIN      
        1 ccattgcggc gatccagatc agcaggtagg acttgcggcc gctgtcgaag acccagcccg
       61 ccactgaaat tgccggcaca ttgtcgatcc cgctgtactg gccgagcagg ggcaggttgc
      121 cgaacaggta gtagatcgcg atgccccagg cgatggtggc gagcgacagg taatgccccg
      181 acaggcgcag cgtgacccag ccgatcagcg ccgcgcaggc agcgaccagc agcaggctgg
      241 cggcgaggcc gagccagggc gatccgcctg ccagcaccgt gagcgccgcg gtggcgtagg
      301 cggcgatgcc gacgaatgcc tgctggccga acgaaacgat gccggccacg cccgtcatga
      361 caacgaggcc aagcgtcacc agcgtcgcca gcccgatgta gttcaacatc gtgatcggga
      421 actcgggcaa cagccagggt ggcagcacca gcaccagcaa ggcgcccagc caccagcgcg
      481 cggagcctgt catccaggtc cttgcccgcg gcgggtcatg ctcgcctgcg gcgctcattc
      541 ttcctcgtcc tcaaccaccg ggtgccgcga ggtgagcgag cgccacagca gcaccgggat
      601 caggaaaccg aacacgatgg tttccttcag cgggctggcc cagaacgagg catacgcttc
      661 cagcaggccg accaccaacg cgcccaccgc ggccagcgga tagctcgcca ggcccccgag
      721 gatggcgccg acgaagccct tcaggccgat catcaggccg gtgtcgtagt agatggtcgc
      781 gatcggcgaa atcaggatgc ccgacagcgc accgatcgcc gccgccagcc agaacgcgat
      841 ctcgccggcc gtggctgtgg gaatgccgac caggcgcgcg ccaacacggt tcatggccgt
      901 tgcccgcaac gccttgccgg tgagcgagcg ttcgaaaaag gcccatagcg cggccatgca
      961 cagcagcgtc gccgccagca cccacaggct ctgcgcgctg atctgcagca cgcccagcgg
     1021 gaatttggca tcggagatcg ggttggtgcg cagcccctcg ccgccgaaga tcgcgagcgc
     1081 gagacccgac agcgcgtagt gcgaggccat cgagatgata aacagcacca gcaccgaagc
     1141 atcggccagc ggccggtacg ccagacggta cagcatcggc ccgagcggga tcaccagggc
     1201 catcgtcaac gcgatcagcc acaccgcatc gagcttgcgc ggcgcggccc acgcggtgag
     1261 cgcagccacc gcgaccggca gcgcgacatt gaccagcacg atgcgcagca ggcgccgcgg
     1321 cgcgccttcg cgccaggcgg cgtagccatc gagcaccgcg atcaaggcgc cgccgcccac
     1381 cagcaggtac accgtcccgg gcatcactcc gagctgcagc gtgcccagcg tcagcccggc
     1441 ataggagatg aagtcgccct gcggtacgaa gatgatgcgg gtgaccgcga ataccagcag
     1501 cagcgccagc gccagcaggg cgtagatcgc gccggtggtg acaccgtcct ggatcagcca
     1561 gagggcgatg tcggagttca tcgattcgga attcgttcgg tgaaatgagc cagaggccgg
     1621 catgccggcc tctgctcgct tcacattact tcatcagccg ccattcgccg ttcacgacct
     1681 gcaccagcac gcgcgcgcgc tcgtccaggc cgttgtgatt ggccggcgac aggttgtaga
     1741 cgccatgcgt gccgaccacg ttcttgacgt tctccagtgc ggcgcgcaac gcctcgcgga
     1801 attccggcgt gccgggcttg gccttcttca gcgccacacc ggcggcggca tcgagcagca
     1861 tcacgccgtc gtaggtgtag ccggcgaacg cgttgcggct ggaggggccg aaagcggcct
     1921 cgtagcgctt gacgaaatcg agcgagactt tcttcgtcgg gaaattgtcg ggcagctcct
     1981 cggccacgat cagcgcgccg gtgggcgcga tcgcgccttc ggccgccttc ttgccggtct
     2041 ggatgaaggg caggttcacc gtgccgtggt tgtgatagat cggccccttg aaaccgcgct
     2101 cggacagcgc gatctgcggg gtcgcgccgg gagaccccga gccgcccacg atgaccgcgt
     2161 ccggcgaagc ggccatgacc ttcagcacct g
//
LOCUS       DASWIY010000433         5840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1052541, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000433 DASWIY010000000
VERSION     DASWIY010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052541"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VGA59_15365"
     CDS             <1..324
                     /locus_tag="VGA59_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEX9721097.1"
                     /translation="QEHPEYDAELADVEAALVRAYDGQDGRANPFLHLAMHLSISEQC
                     SIDQPRGIRKAVELLAARRDSLHDAHHEAMECLGQMLWESQRSGQPPDGNAYIDCVER
                     RATKN"
     gene            complement(390..1103)
                     /locus_tag="VGA59_15370"
     CDS             complement(390..1103)
                     /locus_tag="VGA59_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9721098.1"
                     /translation="MTKLLTMLFAIAVASVTGLAQAQAPAAAPAPAQQNAAAAPASGG
                     SRSIEAKVAACIGCHGIKGYQASFPEVYKVPMIAGQSARYIASALAEYKQGNRKHPTM
                     RSVAATLSDQDIAEIAAYYEALGKDPADTLPASADKAPNPQVAALLQKAACVSCHGAN
                     FAQPIDPSYPKIAGQHADYMFVALKAYKTDDNPSVGRTNGVMGAIAKQFSNAELKALA
                     GYVSSLEGDLKTVPERKFR"
     gene            1195..2049
                     /locus_tag="VGA59_15375"
     CDS             1195..2049
                     /locus_tag="VGA59_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9721099.1"
                     /translation="MKFQGSQNYVATQDLMLAVNASITLKRPLLVKGEPGTGKTMLAE
                     EVAQALNVPLLQWHIKSTTKAQQGLYEYDAVSRLRDSQLADVEGSERVKNIHNYIVKG
                     VLWQAFTADRPVALLIDEIDKADIEFPNDLLRELDRMEFFVYETRELVKARHRPLVFI
                     TSNNEKELPDAFLRRCFFHYIRFPDADTMKQIIDVHFPGLKSQLLTAAMKTFYDVRNL
                     PGLKKKPSTSELLDWLKLLVAEDIPLEALQSKDEKVSVPPLVGALLKNEQDVTLFEKL
                     VFMQRHNR"
     gene            2060..2668
                     /locus_tag="VGA59_15380"
     CDS             2060..2668
                     /locus_tag="VGA59_15380"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX9721100.1"
                     /translation="MRRPESCTLETPPVRLEPLAAGHAADLEAAARDGELWNLRVTSV
                     PAPGETTAYVAAALKGRDEGHMLPFVVRDLPSGRVIGCTRYHDIVPAVERLEIGYTWY
                     GKSWQRSHVNTTCKLLLLAHAFEALGAKLVGWRTDNYNFASQAAIERLGARKDGVLRH
                     HALRRDGTVRDTVMYSLAAGEWPEVKAHLQYELSRPRGSQSC"
     gene            2662..3852
                     /locus_tag="VGA59_15385"
     CDS             2662..3852
                     /locus_tag="VGA59_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9721101.1"
                     /translation="MLIDFFYTLRSAKLPVSVKEYLMLLEAVQKGVVGPQSEDGYKID
                     DFYYLARTALVKDEKHFDKFDRAFAAYFKGVEMVADFTKDIPLEWLRKNLELELSPEE
                     KAKIEKMGWDELMETLKKRFEEQKERHEGGSKMIGTGGTSPFGAYGFNPQGIRIGQDK
                     GRNRSAVKVWDQRAYKDYDDTQELGTRNIKIALRRLRKFAREGAAEELDLDDTIKSTA
                     ANAGYLDIKMVPERHNNVKILLLMDVGGTMDEHIHRVEEMFSAAKAEFKHLEFYYFHN
                     CVYDFMWKNNRRRFSEKFATWDIIRKYNKDYKLIFVGDATMSPYEILQPGGSVEYNNE
                     EAGVEWLQRLTGAFPKFAWVNPEPQGVWQYRQSISVVQQLMNQRMYPLTIKGLEEAMR
                     LLSK"
     gene            3914..3988
                     /locus_tag="VGA59_15390"
     tRNA            3914..3988
                     /locus_tag="VGA59_15390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3947..3949,aa:Arg,seq:cct)
     gene            4246..4473
                     /locus_tag="VGA59_15395"
     CDS             4246..4473
                     /locus_tag="VGA59_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721102.1"
                     /translation="MTSDFRVESNHPIAGRLFPVSGSQQYFFTDRALAVAMAAKSVTS
                     PSGQEIRVVHVPTGEVVFRKAGSSRGEFNDE"
     gene            complement(4460..>5840)
                     /locus_tag="VGA59_15400"
     CDS             complement(4460..>5840)
                     /locus_tag="VGA59_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX9721103.1"
                     /translation="AYVVAYAYTDYLQFSGPLQSWLRASFGLQGRVFPEVRSLGGAAV
                     VFVFSLYPYVYLLARTALGERAGHLMEAARLLGAPLRRRIRQVALPLARPAIAAGVAL
                     ALMETLADYGVSSYFGIQTFTTGIYKAWLAMDNRIAAAQLATLLLGVVALLLALERRA
                     QRRLRFATPRGHRPGSEAQPLPLRGARLLLAWTLCGLPVLLGFVLPVLFMLRPLAADW
                     SVLPWDRFAQWAFNSVRLGVITALLAVAIALLMAFSLRRAPDRITRGVVQLAALGYAV
                     PGAVIVVGLLLPVGWLQEAAPRSGIGGWVTATILGVVWAYLVRFVSVALQSVQSGYTR
                     IPGSFDDSARMLGTGGFGLLARVHWPLLRRSATAAALLVFVDVMKELPATLVLRPFDT
                     DTLAVVAYQLARDERLGEAALPSLALVLVGLLPVILLSRTLRSRPPVANPVAPNTGTA
                     MLDAGFTRR"
BASE COUNT         1166 a         1890 c         1865 g          919 t
ORIGIN      
        1 caggagcatc cggagtacga cgccgaactt gccgatgtcg aggccgcgct ggtgcgcgcc
       61 tacgatgggc aggacggccg cgcgaatccg ttcctgcatc tcgccatgca cctgtcgatc
      121 agcgaacagt gctcgatcga ccagccgcgc ggcatccgga aggcggtcga actgctggcc
      181 gcgcgccgcg actcgctgca cgacgcgcac catgaagcca tggagtgcct gggccagatg
      241 ctgtgggaaa gccagcgctc cggccagccg cccgacggca acgcctacat cgattgcgtg
      301 gaacgccgcg ccacaaagaa ctaaaccgaa aagtacaagg cccgctacgc gggccttgtt
      361 tcgtgttggg gcgtagcggt ttcctgtcct taccggaact tccgctccgg caccgtcttc
      421 aggtcgcctt cgagcgagct cacgtaaccc gccagcgcct tcagttcggc attggagaac
      481 tgcttggcaa tggcgcccat gacgccgttg gtgcggccga cgctgggatt gtcatcggtc
      541 ttgtaggcct tgagcgccac gaacatgtaa tcggcgtgct ggccggcgat cttgggatag
      601 ctggggtcga tgggctgggc aaagttcgcg ccgtggcagg aaacgcatgc ggccttttgc
      661 agcagcgcgg cgacctgcgg gttcggcgcc ttgtccgccg aggccggcag ggtgtccgcc
      721 gggtccttgc ccagcgcttc gtagtatgcg gcgatctcgg cgatgtcctg atccgacagg
      781 gtggccgcca cgctgcgcat ggtggggtgc ttgcggttac cctgcttgta ctccgccagc
      841 gccgaggcga tgtacctggc gctctggccg gcgatcatgg gaaccttgta gacctcgggg
      901 aagctcgcct ggtagccctt gatgccgtgg catccgatac acgcggccac cttggcttcg
      961 atcgatctgg aaccccccga tgccggcgcg gcagcggcgt tctgctgggc cggggctggg
     1021 gccgcggccg gcgcctgtgc ctgtgccaaa cccgtcacgg aagcgacagc gatggcgaag
     1081 agcatggtca acaacttggt cattttgcga gcacaatcgg gtgagaggtc agtgttctga
     1141 tcaacctttg attatatgcg gcggctttcg cgccgtttcc actccaatat ctccatgaag
     1201 ttccaaggct cacagaacta cgtcgccacg caagacctga tgcttgccgt gaacgcgtcc
     1261 atcacgctca agcgcccgct gctcgtcaag ggcgaacccg gcaccggcaa gaccatgctg
     1321 gccgaggaag tggcccaggc tttgaacgtg ccattgctgc agtggcacat caagtcgacc
     1381 accaaggcgc agcagggcct gtacgaatac gacgccgtca gccggctgcg cgactcgcag
     1441 ctggccgacg tggagggctc ggagcgcgtc aagaacattc acaactacat cgtcaagggc
     1501 gtgctgtggc aggccttcac ggccgaccgg ccggtggcgc tgctgatcga cgaaatcgac
     1561 aaggccgata tcgaattccc gaacgacctg ctgcgcgagc tcgaccggat ggagttcttc
     1621 gtttacgaga ctcgcgaact ggtcaaggcc aggcaccgcc cgctggtgtt catcacctcg
     1681 aacaacgaga aggagctgcc cgacgccttc ctgcgccggt gcttcttcca ctacatcagg
     1741 ttccccgacg cggatacgat gaagcagatc atcgacgtgc atttcccggg cctgaaaagc
     1801 cagctgctga cggcggccat gaagacgttc tacgacgtgc gcaacctgcc gggcctgaag
     1861 aaaaaaccgt ccaccagcga gctgctggac tggctcaagc tgctcgtggc cgaggacatt
     1921 ccgctggaag cgctgcagag caaggacgaa aaggtgtcgg tgccgccgct ggtgggcgcc
     1981 ttgctcaaga acgagcagga cgtgaccctg ttcgaaaaac tggtgttcat gcagcgccac
     2041 aatcgctgaa aggcgaatga tgcgaaggcc tgaaagctgc acgctggaga cgccgccggt
     2101 gcggctggag ccgctcgcgg ccggccatgc ggcggatctc gaggcggccg cgcgcgacgg
     2161 agaactctgg aacctgcgcg tcacttcggt gcccgcaccg ggcgagacca ccgcctatgt
     2221 ggccgctgcg ctgaaggggc gcgacgaggg ccacatgctg ccgttcgtgg tgcgcgacct
     2281 gcccagcgga cgggtcatcg gctgcacgcg ctatcacgac atcgtgccgg ccgtcgaacg
     2341 cctggagatc ggctacacct ggtacggcaa gagctggcag cgcagccacg tgaacacgac
     2401 ctgcaagctg ctgctgctcg cgcacgcctt cgaggcgctg ggtgcgaagc tggtgggttg
     2461 gcgtaccgac aactacaact tcgcgagcca ggccgccatc gagcgcctgg gcgcgcgcaa
     2521 ggacggcgtg ctgcgccacc acgccctgcg gcgtgacggc accgtgcgcg acaccgtcat
     2581 gtacagcctc gcggcgggcg aatggcccga ggtcaaggcc cacctgcaat acgagctctc
     2641 caggccaaga ggatctcagt catgctgatc gacttcttct atacccttcg cagcgccaag
     2701 ctgccggtct ccgtgaagga atacctgatg ctgctcgaag ccgtccagaa gggcgtcgtc
     2761 gggccgcaga gcgaagatgg ctacaagatc gacgacttct actatctcgc gcgcacggca
     2821 ctggtcaagg acgagaagca tttcgacaag ttcgaccgtg cctttgccgc gtatttcaag
     2881 ggcgtggaga tggtggcgga tttcaccaag gacatcccgc tggaatggct gcgcaagaac
     2941 ctcgagctcg agctcagccc cgaggaaaaa gccaagatcg aaaagatggg ctgggacgag
     3001 ctgatggaga cgctgaagaa gcgcttcgag gagcagaagg aacgccacga aggcggcagc
     3061 aagatgatcg gcaccggcgg cacttcgccc ttcggtgcct atggcttcaa tccgcagggc
     3121 atccgcatcg gccaggacaa gggccgcaac cgcagtgccg tgaaagtgtg ggaccagcgc
     3181 gcctacaagg actacgacga cacgcaggaa ctgggcacgc gaaacatcaa gatcgcgctg
     3241 cggcgcctcc gcaaattcgc gcgcgaaggc gcggccgaag aactggacct ggacgacacc
     3301 atcaagtcga cggccgccaa tgccggttac ctggacatca agatggtgcc cgagcggcac
     3361 aacaacgtga agatcctgct gctgatggac gtgggcggga cgatggacga gcacatccac
     3421 cgcgtcgagg agatgttctc ggccgcgaag gccgagttca agcacctgga gttctattac
     3481 ttccacaact gcgtctacga cttcatgtgg aagaacaacc gccggcgctt ctccgagaag
     3541 ttcgcgacgt gggacatcat ccgcaagtac aacaaggact acaaactcat cttcgtcggc
     3601 gacgcgacga tgagcccgta cgagatcctc cagcccggcg gcagcgtgga atacaacaac
     3661 gaggaagccg gcgtcgagtg gctccagcgg ctgaccggcg cgttccccaa gttcgcctgg
     3721 gtcaatcccg aaccgcaggg cgtctggcag taccgccaga gcatcagcgt ggtccagcag
     3781 ctgatgaacc agcgcatgta ccccctgacg atcaagggcc tggaagaggc catgcggctg
     3841 ctgtcgaagt agccggcagg tcccccgcgg ccgggacggc gccgggttgc gccatttaca
     3901 atggcgcggc cccgccgccg tagttcaatg gatagaacga gcgcctccta agcgctagat
     3961 acaggttcga ttcctgtcgg tggtaccaac gctgcccttg gaccgctttg tttgcgttgc
     4021 atcaaagaac ctgaggagtc cgcacgcatc ctacgtccgt catcggcaac gtcctacaga
     4081 caaccgaacc tcagtcgtta gcgtattgcg acgcaccatt gctcgtggtt acacctctga
     4141 cgacgacctt gcattcgggt cattgctatg tcatgggacc tgtctaccat cacttgagca
     4201 gtgcaaagca gaatcccgag agacataaaa gagaggttta tccccatgac gtccgacttc
     4261 cgcgtcgaaa gcaatcatcc catcgctggt cgtttgttcc cggtttccgg ttcccagcag
     4321 tattttttca ccgaccgcgc cctggctgtg gcgatggccg ccaagagcgt caccagtccg
     4381 tcgggtcagg aaattcgagt cgtccatgtg cctaccggtg aggtcgtttt cagaaaagcc
     4441 ggctccagcc gaggcgagtt caacgacgag taaagccggc gtcgagcatc gccgtccccg
     4501 tgtttggcgc caccgggttc gccaccgggg ggcgtgaacg cagggtgcgg ctcaagagga
     4561 tcaccggcag cagcccaacc agcaccagcg caagcgacgg cagcgccgct tcgcccaggc
     4621 gctcgtctcg cgcaagctgg taggccacta cggccaacgt gtcggtatcg aaaggccgca
     4681 ataccaatgt ggccggcagc tccttcatga cgtcgacgaa caccagcaga gccgcggccg
     4741 tggccgagcg cctgagcagc ggccaatgga cccgggccag caggccgaag ccaccggttc
     4801 ccagcatccg cgcgctgtcg tcgaagctac ccgggatgcg cgtatagccg ctttgcacgg
     4861 actgcagcgc cactgaaacg aaacgcacga gataggccca gaccacgccg agtatggtgg
     4921 cagtgaccca gccgcctatt ccggagcgcg gcgccgcctc ttgcagccag ccgaccggca
     4981 gcagcaggcc gaccacgatc accgcgcccg gtaccgcgta gccgagcgcc gccaactgga
     5041 cgaccccgcg cgtgatcctg tccggcgcgc gccgcaggct gaaggccatc agcagcgcga
     5101 tcgcgacagc cagcaaggcg gtgatcacac ccaaccgcac gctgttaaag gcccactgcg
     5161 cgaatcgatc ccatggcagc accgaccagt ccgcggccag cggacgcagc atgaacagca
     5221 ccgggagcac gaaacccagc agcaccggca ggccgcacaa cgtccaggcc agcaacagcc
     5281 gtgcgccacg cagcggcagc ggttgcgcct ccgagccggg ccggtggccg cgcggcgtag
     5341 cgaaacgcaa acggcgctgc gcccgccgtt ccagcgccag cagcagcgcc accacgccca
     5401 gcagcagcgt cgcgagctgc gccgccgcga tgcggttgtc catcgccagc catgccttgt
     5461 agatgccggt ggtgaaggtc tggatgccga agtagctgga cacgccgtag tcggccagcg
     5521 tttccatcag cgccagggcg acaccggcgg caatggcggg acgggcgagc ggaagcgcga
     5581 cctggcggat gcgccggcgc aggggcgcgc ccagcagccg cgccgcctcc atcaggtgac
     5641 ccgcgcgctc gccgagggcc gtgcgggcca gcagatagac ataggggtag agcgagaaca
     5701 cgaacaccac ggctgcgccg cccaggctgc gcacttccgg aaagacgcgg ccctgcaggc
     5761 cgaagctggc acgcagccac gactgaagag gcccgctgaa ctgcagatag tccgtgtagg
     5821 cgtaggcgac cacgtatgcc
//
LOCUS       DASWIY010000434         9843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1411674, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000434 DASWIY010000000
VERSION     DASWIY010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9843
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411674"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VGA59_15405"
     CDS             <1..282
                     /locus_tag="VGA59_15405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX9721104.1"
                     /translation="PTVGSEIKKARPCVVVSPAELNDHLRTVIIAPMTSKGFAAPFRI
                     PVTHAGTKGLILLDQIRTVDKVRLAKRLGAVSAKALGATLMTLQEVFAA"
     gene            complement(380..814)
                     /locus_tag="VGA59_15410"
     CDS             complement(380..814)
                     /locus_tag="VGA59_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9721105.1"
                     /translation="MPNIGTVLKSEIARIARKEVRGETQQLRKQGAQYRSEIAALKKR
                     LAGMEQQLKRQLKAGSPRSAPEAQEPRKGFRFSAKGLAARRQKLGLSAREMGALLGVS
                     AQSVYHWEQGKSRPRASQVQAISGLRRLGKREASARLSALGT"
     gene            1147..2049
                     /locus_tag="VGA59_15415"
     CDS             1147..2049
                     /locus_tag="VGA59_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9721106.1"
                     /translation="MKRRTLLLHLLPALATVDAVAQSPAPARPAAKPIPSSGEEIPVL
                     GLGSWITFNVGNDPPARERCAEVMRHFFSAGGRLIDSSPMYGSSQGVIGDGLQRLAAQ
                     GRVFSADKVWTSSNGAPQIEASRGHWRAARFDLLQVHNLLAWQEQLPLLQAMKAAGRL
                     RYVGITTSEGRRHRDMEQIMRNHRIDFVQLTYNLLDREAEQRLLPLARERGLAVLANR
                     PFREGALLRHLRRHPVPAWAAEIDCASWPQFALKFIVSHPAVTCAIPATGSVAHVSEN
                     MAASIGRMPDEAFRRRMAAHVEKL"
     gene            2053..2646
                     /locus_tag="VGA59_15420"
     CDS             2053..2646
                     /locus_tag="VGA59_15420"
                     /inference="COORDINATES: protein motif:HMM:NF039641.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6064 family protein"
                     /protein_id="HEX9721107.1"
                     /translation="MSEWWTYRPSDFMMFSPRAYWRLVEQYNGDLWPAQLVGVAAGLV
                     LLWLAAVPRSVASRIVAASLALAWLWVGWAFHWQRYATINWAAEYFAVVFWVQAMLLV
                     ILGVFQRDGQLQPSAGVPNLGWTLAVCGVVLYPLAGLAAGRPWSQAEVFGVMPEPTAL
                     ASLGLLLSGGQPYAKALSILPALSFLVGMTTLWILSG"
     gene            2878..3153
                     /locus_tag="VGA59_15425"
     CDS             2878..3153
                     /locus_tag="VGA59_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721108.1"
                     /translation="MMRYSRAEHARVQALQQEVSRAEADYQRLRAAYLEIAKNEPGHE
                     VALAMIGADMDRAHAHLQALIGLPRLPFTHEPSTVVRREAQRLAENH"
     gene            3246..3410
                     /locus_tag="VGA59_15430"
     CDS             3246..3410
                     /locus_tag="VGA59_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721109.1"
                     /translation="MIPYDIDPSLHTAEPESQVLDDGFSPTRHGAPAATAQVPPADVV
                     PTDSDLSGQS"
     gene            3579..3752
                     /locus_tag="VGA59_15435"
     CDS             3579..3752
                     /locus_tag="VGA59_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721110.1"
                     /translation="MKTAIATGLTPPRPGLEREEPELAYEHDIPPDDGTAMPPDEPPA
                     ESEEQPLRQIERE"
     gene            3758..3910
                     /locus_tag="VGA59_15440"
     CDS             3758..3910
                     /locus_tag="VGA59_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721111.1"
                     /translation="MTSAPAPAAPAPAPTHPPKPVDPEPLQEPTDPEKGRVKTPEPGK
                     SDHPSQ"
     gene            complement(3950..4705)
                     /locus_tag="VGA59_15445"
     CDS             complement(3950..4705)
                     /locus_tag="VGA59_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721112.1"
                     /translation="MSLFSPAFASAPALGKPHARRLREIYRSAGWPCQDPLEIELVAA
                     GLLDRVRAPSGHESLRVTDAGVRLLAATLAGNRASFSAHEALVERVALEMGRAGRIAW
                     RGLGLRAQVPAQDGAAPTRWCVARPDVFSIRNTSVATYVDPIVHEVKVRRADLLGDLR
                     RAAKRAAYLDLGECWYVLGCDARGRSIAAPEEVPAECGVLLLEQGRLVVARAAPRRSR
                     NGLPFGVWMALAKASPIGGLDEAAQALLGGAAG"
     gene            4839..5147
                     /locus_tag="VGA59_15450"
     CDS             4839..5147
                     /locus_tag="VGA59_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721113.1"
                     /translation="MDEKAVYGITALRYAGAQLVEAMMGLIDSAQASWDLRPTPARLS
                     EVVDRLVEGDTIISLFPDDMGRLQPGPEVKVDVLPEGTETLALAEEPPGRSLRDLPRF
                     "
     gene            complement(5422..5916)
                     /locus_tag="VGA59_15455"
     CDS             complement(5422..5916)
                     /locus_tag="VGA59_15455"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX9721114.1"
                     /translation="MRFDHSIVQTRFESPLGPMIVAATHRGLAGLWFEGQRHLPDCSA
                     WPHQPRHPVLRQAVAQLDEYFSGTRKRFDLPLDLQGGTVFQQSVWQALLAIPCGGTTS
                     YGMLSRRIGQPSAVRAVGAAVGRNPLSIVVPCHRVLGADGSLTGYAGGLERKSALLRL
                     EGTR"
     gene            complement(5973..7565)
                     /locus_tag="VGA59_15460"
     CDS             complement(5973..7565)
                     /locus_tag="VGA59_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX9721115.1"
                     /translation="MTRDAAPLQADSCYLALKARDARFDGCFFTAVTSTGIYCRPVCR
                     VRTPKRENCRFFRHAAQAESAGFRPCLRCRPELAPHSVIWSIQDASHILAHQAARLLD
                     DPEAFGDTPPSVEQLAGRLGVSDRHLRRIFEAQFGVSPVQYLQTRRLLTAKQLLTDTE
                     LAVTQVALASGYSSVRRFNAAFLQHYGLNPTRLRRDGAARPAQGMAIRLGYRPPYDVR
                     AMLDFFGRRAIPGIEFVNAAGTATGRTLAIEAGGKSHRGWLLAEFDEDRHQVVLRVSD
                     TLREVLPLVIHRLRCALDLDADPRAINSVLHASFPQGDGLRVPGAMSGYEVAVRAVLG
                     QQVTVAAARTLAQRLVDRFGEPLATPRPELTRLFPEPAVLARAGGDALGQLGIVKQRQ
                     AAIVAIAQAVADKRLQLHGGVDVHATIEALKQRPGIGDWTAQYIAMRALRWPDAFPAG
                     DVALQKALGVQGGKSPARAAQSASQAWKPWRSYAVIRAWNQPPKPDDSCRAGLEGLAP
                     ERDRGASREPGLRVKPAMTSPQ"
     gene            complement(7646..8758)
                     /locus_tag="VGA59_15465"
     CDS             complement(7646..8758)
                     /locus_tag="VGA59_15465"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX9721116.1"
                     /translation="MNLYRPLLRPLLFRAEAETMHCLAIRAAELASSSRWLCSSVARR
                     HCADSERLEVAIGGLRFRTPLGLAAGFDKSARAVPFLAALGFGHVEVGSISAEPSAGN
                     PKPRLFRIPQDRGIVVNYGLPNDGAERVAQRLAGLRACAPLGINIVSTNRGAAAAAEP
                     DDAVIADYLQSVRRLQPVADYLCLNLSCPNTREGRGFFHEPARLAQLLQGLDSLDGRG
                     VARPLFLKVAPFQSPAELETFLATVKPARSVTGFSVNLPPGKPGGLRTPAWQLGAMPG
                     AVSGPPAMAAADRTLSELYRQMDRSRYRLIGSGGVFTAADAYRKIRLGASLVQLMTAL
                     MYEGPGVAAAINRELPRLLQADGVRHVSEAVGVDVL"
     gene            8850..>9843
                     /locus_tag="VGA59_15470"
     CDS             8850..>9843
                     /locus_tag="VGA59_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX9721117.1"
                     /translation="MQFSPSIFKAYDIRGVVPSTVNEEMAQALGWAFGKRAQAEGEKT
                     VAVGRDGRLSGPALVAALVRGLAASGVDVIDIGPATTPMLYFAASTLCSSGVQVTGSH
                     NPKDYNGFKMVIAGRAIHGQEIQALRPLMQAWSPEAAATAGPGRVRNINILAPYRDRI
                     VQDAKLARPMKIVVDSGNGIAGASAPGIFRALGCEVVELFSEVDGTFPNHHPDPSKPE
                     NLKDLIAAIRASGAELGLAFDGDGDRLGIVTRGGNNIYPDRQMMLFARDVLTRVPGGT
                     ILFDVKCTQRLAPAIREAGGVPLMYKTGHSLIKAKMKEVASPLGGEMSGHIFFKD"
BASE COUNT         1573 a         3366 c         3371 g         1533 t
ORIGIN      
        1 cccactgtcg gcagcgagat taagaaggcc cgtccttgcg tagtggtgtc gccggcagag
       61 ctgaacgatc accttcgcac cgtcatcatc gccccgatga cctccaaagg cttcgcggcg
      121 ccttttcgga ttccggtgac tcacgccggg acgaaggggc tgatcctgct ggatcagatt
      181 cgaacggtag acaaggtgcg cttggcgaag cggctgggtg cggtttcggc gaaggcgctc
      241 ggcgcaactt tgatgacgct gcaagaggtg ttcgcggctt gatttgaaac ggatcgggaa
      301 ctggcgtccc taggcatccc cgagtcccgc ggcgaacacg gcgagcacgg gccctcgcgt
      361 ggcgacgcgc ccgcgccggt caggtcccca gggcgctcaa tcgagccgat gcctccctct
      421 tgcccaagcg ccgcaagccc gaaatggcct gcacctggct cgccctcggc cgggacttgc
      481 cttgctccca gtgatacacg ctctgcgccg acactcccag caaggcgccc atctcgcgtg
      541 cggagagtcc gagcttctgc ctgcgtgccg ccaggccctt cgcgctgaac ctgaaaccct
      601 tgcggggttc ctgcgcctcg ggggccgagc ggggagagcc ggccttgagt tgtcttttga
      661 gctgttgctc catcccggca aggcgcttct tcagggcggc aatctcggag cggtactgcg
      721 ctccctgctt cctgagctgc tgcgtttccc ctcggacttc cttgcgcgcg atccgcgcta
      781 tttcactttt gaggacggta ccgatatttg gcatttttgt cgtttcgata agaggaaagc
      841 ctaatggtag cgatttgtgc ggctcgtggc ggacgaaaga aagccccgcg gcgcggggct
      901 ttctcgttta tcgggcttcg ccgcctgcgg cggggcccac gccgcaggac ggattcagtg
      961 catttccgcc ctgctgtcgg tgccgccggt ggaggccgtc acggcggttc gccacgcgtc
     1021 catcgcgctc gtgaagccgc ccttcgtggg catggaattc ctttcaagga ggaaagtaca
     1081 agctggtccg tggtaacagc aggaaaatcg ctccatgtcc accgaacgaa cggctacgct
     1141 gctcgaatga aacgccgcac gttgctgttg cacttgctcc ccgcgctggc cacggtcgac
     1201 gcggtcgcgc aaagccccgc gccggcacgg cctgccgcca agccgattcc ctcgtcgggt
     1261 gaggaaattc ccgtgctggg gctggggagc tggatcactt tcaacgtggg caacgatccg
     1321 cccgctcgcg agcggtgcgc ggaggtcatg cgccacttct tctccgcggg cggccgcctg
     1381 atcgattcat ccccgatgta cggttcgtca caaggcgtga tcggcgacgg cctgcaaagg
     1441 ctggcggcgc aggggcgggt cttttcggcc gacaaggttt ggacatcgtc gaatggcgcc
     1501 ccgcagatcg aggcatcgcg cgggcactgg cgggcagccc gattcgacct gctgcaggtg
     1561 cacaacctgc tggcctggca agaacagctg ccgctgctgc aggcgatgaa ggccgcgggg
     1621 cggctgcgct atgtgggcat taccacctcg gagggccggc gccaccgcga catggaacag
     1681 atcatgcgaa accaccggat cgattttgtc cagctcacct acaacctgct cgaccgcgaa
     1741 gccgagcagc ggctgctgcc gctggcgcgt gagcggggcc tcgccgtgct ggcaaaccgg
     1801 ccgttccgtg aaggcgcgct gctgcgccac ttgcggcgcc accccgtgcc ggcctgggcc
     1861 gccgagatcg attgcgccag ctggccccag ttcgcgctga agttcatcgt gtcgcatccc
     1921 gccgtcactt gcgccatacc ggcgaccggc agcgtcgccc atgtgagcga gaatatggcg
     1981 gcttcgatcg ggcgcatgcc ggacgaagcc ttccgcaggc gcatggcggc ccacgtcgag
     2041 aagctctgag ccatgtccga gtggtggact taccggccgt ccgatttcat gatgttctcc
     2101 cccagggcct attggcgcct ggtcgaacaa tacaacggcg acctctggcc ggcgcagctc
     2161 gtcggggtgg cggccgggct ggtgttgcta tggctggccg ccgttccgcg ttccgtcgcc
     2221 agccggatcg tcgccgcctc gcttgcgctt gcctggctct gggtgggctg ggctttccat
     2281 tggcagcgtt acgccaccat caactgggcg gccgagtact tcgccgtcgt gttctgggtc
     2341 caggccatgt tgttggtcat tctgggcgtg ttccagcgtg acggacaact ccagccgagc
     2401 gctggtgtgc ccaacctggg gtggacactg gccgtctgcg gggtggtgct ctatccgctg
     2461 gcgggtcttg cggccggccg gccctggtcc caggccgagg tcttcggcgt gatgccggag
     2521 ccgacggccc tcgccagcct gggcctgttg ctgtccggcg gccagcctta tgccaaagcg
     2581 ttgtccatcc ttccggcgct ttcgttcctg gtcggcatga cgaccctgtg gattctgtcg
     2641 gggtgaaagc gcgagcccgc cggcgggcgg cgcgtttgcc ggatctggcg ctcggccggc
     2701 tgagcaggca ggttcagccg tagacggacc gcgcctgcgc ccgctgtctg acttgtccta
     2761 catcgggaaa atgtccggct cgatacgatg tgctcatgag tcgacgcgtc catcgcatga
     2821 acccgacggt cgaggccagg gggagcagac gcgacggcac caaaatggtt gagccgcatg
     2881 atgcgttata gccgtgccga acacgccagg gtgcaggccc tgcagcagga ggtctcgcgt
     2941 gccgaggccg attaccagcg cctgcgcgcg gcttatctgg agatcgcgaa aaacgaaccc
     3001 gggcatgagg tcgcgctggc catgatcgga gccgacatgg accgggcgca tgcccatttg
     3061 caggcgctga tcggtctgcc gcggctgcct ttcacgcatg agccgagcac cgtggtgcgc
     3121 cgcgaagccc agcgcctcgc cgagaatcac tgaccgcacg tcggcatgcg ccgacacgga
     3181 caaggtgctt cggccgaggg gcgggcgacc tctcgggcgt gactattggc cgaaaggagt
     3241 ccgccatgat cccgtacgac atcgacccca gcctgcacac cgccgaaccc gaatcgcagg
     3301 tgctggacga cggcttttcg ccgacccgtc acggcgcgcc ggctgcgacg gctcaggtgc
     3361 cgcccgcgga cgtcgttccg acggattcag acctgtcggg ccagagctag gcagccatgg
     3421 caactcatcc tgtcgagccc gagccgctac ccccggactc ggaacctacc gcgcccaacc
     3481 cgtcgcacct gccggtcgag cctgagttcg gcccaccgca acctgccgag ccggaggatc
     3541 cgcacggcaa gccacccact ctttgaggag agccgagcat gaagacagcc atcgcaaccg
     3601 ggctgacacc gccccgtccc gggctggaac gggaagagcc ggaactcgcc tatgagcatg
     3661 acatcccccc ggacgatggc acggcgatgc cgcccgacga gccgcctgcc gaaagcgaag
     3721 agcagccgct gcggcagatc gaaagggaat gatcgtcatg accagcgcgc cggcacccgc
     3781 cgcccctgcg cccgccccta cgcatccgcc caaacctgtc gatcccgaac ccttgcagga
     3841 gccgaccgac ccggagaaag gccgggtcaa gacacccgaa cccggcaagt ccgaccatcc
     3901 cagccagtga gcggccaggc gccgaccggc gctggttacg ccgcagccgc taaccggcgg
     3961 cgccgcccag caaggcctgc gccgcctcgt ccaggccgcc gatcggactg gccttggcca
     4021 gcgccatcca cacgccaaag ggcaggccgt tgcgcgagcg acgcggtgcc gcgcgagcca
     4081 cgaccaggcg gccttgctcg agcagcagca caccgcattc ggccggcacc tcctcgggcg
     4141 ccgcgatcga gcggcccctg gcatcgcagc cgagcacgta ccagcactcg cccagatcca
     4201 ggtacgcggc gcgctttgcg gcacggcgca ggtcgccgag gaggtcggcg cgccgcactt
     4261 tcacttcgtg cacgatcggg tccacataag tcgcgacgga ggtgttgcgg atcgagaaga
     4321 catcgggcct ggcgacgcac caacgggtcg gcgcggcgcc gtcctgcgcg ggaacctgcg
     4381 cccgcagccc caggccgcgc catgcgatgc gtccggcgcg ccccatctcc agcgcgacgc
     4441 gttccacaag cgcctcgtgg gccgaaaacg atgcccggtt gccggccagc gtggcggcga
     4501 gcaggcgcac gccggcgtcg gtgacgcgca gcgactcatg gccggagggc gcccgcacgc
     4561 gatcgagcag gccggcagcc accagctcga tctcgagcgg gtcctggcag ggccagccgg
     4621 cggagcggta gatctcgcgc agtcggcgcg catgcggctt gccaagggcc ggcgccgacg
     4681 cgaacgcggg ggagaaaagg ctcatgccat ctgcggaaaa aaagcggtct gtcagtgtag
     4741 cccgccacgg cgacgtgaag cgcgccgtag gcagggtccg acggttcgtg gggccgttgg
     4801 cctatcgcgg gagcgggttt gccggccaca atcgctacat ggatgagaaa gcagtctatg
     4861 ggatcacggc tctgcgctac gccggcgccc agctcgtgga agcgatgatg ggcctgatcg
     4921 acagcgcgca ggccagctgg gacctgcggc cgacgcccgc tcgcctgagc gaagtggtgg
     4981 accggctggt ggagggcgac accatcatca gcctgtttcc ggacgacatg ggccggctgc
     5041 aacccggtcc tgaagtcaag gtggacgtgc tgccggaggg caccgaaacg ctggccctgg
     5101 ccgaggaacc gcctggccgc agcctgcgcg acctgccacg cttctagtcc actgactcag
     5161 tggacgatca ccccgcgccg gcgctaatcg cgcgtgccga gttcatgact ttccctgggg
     5221 cccgcgcagc ctcatcagcc ccgtcgcgtg aacacggggt ttccattgcc ggcgcaactg
     5281 cggcaccagt ccgcgcagcc acagcaacgg caacaggaat accgccgcga tggaggaacc
     5341 cccagatcgc cgacagcagc aagaagtcgg tgccccactt gcgggcggcg acggtgccaa
     5401 cgacggcctg gggcacggcg ctcaacgcgt tccctccagt cgcagcaacg cgctcttgcg
     5461 ttccaggccg ccggcgtagc ctgtgagcga gccgtcggcg cccagcacgc ggtggcacgg
     5521 cacgacgatg ctgagcgggt tgcggcccac ggcggcgccc acggcgcgca cggccgaagg
     5581 ttggccgatg cgccggctga gcatgccgta gctggtggtg ccgccgcaag gaatcgcgag
     5641 cagcgcctgc cacaccgact gctggaagac ggtgccgcct tgcaggtcca gcggcaagtc
     5701 gaagcgcttg cgtgttccac tgaagtattc gtcgagctgg gccacggcct ggcgcaatac
     5761 cggatgccgc ggctgatgcg gccaggccga gcagtcgggc aggtggcgct ggccctcgaa
     5821 ccagagaccg gccaggccgc gatgggtggc cgcgacgatc atcgggccga gtgggctctc
     5881 gaagcgcgtt tgaacaatgg agtggtcgaa tctcattggg actcccgaga ggctgtcatt
     5941 gcgggtgaga gattgtcatt gcgggggact tgtcattgcg ggcttgtcat tgcgggcttg
     6001 acccgcaatc caggctccct ggatgcccca cgatcgcgct ccggggcaag cccctcgagt
     6061 ccggcacgac aggaatcatc cggctttggc ggctggttcc aggcacgtat cacggcatag
     6121 ctgcgccagg gcttccacgc ctgcgacgcc gactgcgcgg cacgcgccgg gctcttgccg
     6181 ccttgcacgc cgagggcctt ctgcagggcg acatctcccg ccgggaaggc atcgggccaa
     6241 cgcagcgcgc gcatcgcgat gtactgcgcc gtccagtcgc ctatgcccgg ccgttgcttg
     6301 agcgcctcga tggttgcgtg cacatcgacc ccgccatgca gctgcagccg tttgtcggcc
     6361 accgcttgcg cgatcgcgac gatcgcggcc tggcgctgct tgacgatgcc aagctgtccc
     6421 agggcatcgc cgccggccct cgccagcaca gccggttcgg ggaacaggcg cgtgagttca
     6481 gggcgcggcg tagccagggg ctcgccgaag cggtccacca ggcgctgggc cagcgtccgc
     6541 gccgcggcca ccgtgacctg ctgtccgagc accgcccgca ccgccacttc atagccgctc
     6601 atcgcgcccg gcacccgcag cccatcgcct tgcgggaagc tcgcgtgcag cacgctgttg
     6661 atggcccggg ggtcggcatc caggtccagc gcgcagcgca ggcgatgaat caccagcggc
     6721 agcacttcgc gcagcgtgtc gctcacgcgc agcacgacct gatgcctgtc ctcgtcgaac
     6781 tcggccagca accagccccg gtgcgacttg ccgccggctt cgatggcaag ggtgcgtccg
     6841 gtcgcggtgc ccgcagcgtt cacgaactcg atgcccggga tcgcgcgccg gccgaaaaag
     6901 tcgagcatgg cccgcacgtc gtaaggcggg cggtaaccga gccggatggc catgccttgc
     6961 gcgggccggg ccgcgccgtc gcgccgcagc cgcgtcggat tcaggccgta gtgctgcagg
     7021 aacgcggcgt tgaagcggcg cacgctggag tagccgctcg cgagggcgac ctgcgtgacg
     7081 gccagctcgg tgtccgtcag cagctgcttg gcggtgagca gccggcgtgt ctggaggtac
     7141 tgcaccggcg agacgccgaa ctgggcttcg aagatgcggc gcagatgacg gtcgctgacg
     7201 cccaggcggc ccgcgagctg ctccacggaa gggggcgtgt cgccgaacgc ctcggggtcg
     7261 tcgagcagcc gtgccgcctg gtgcgccagg atatggctcg cgtcctggat cgaccagatg
     7321 acggagtgcg gcgccagctc ggggcggcag cgcaggcagg gccggaagcc cgcgctctcc
     7381 gcctgcgccg catggcggaa gaagcggcag ttttcgcgct tgggtgtgcg cacgcggcac
     7441 actggccggc aatagatgcc ggtcgaggtg acggcggtga agaaacagcc gtcgaagcgg
     7501 gcatcgcgcg ctttcagcgc caggtaacag ctgtcggctt gcaggggcgc ggcatcgcgg
     7561 gtcatgccgt ggatgatacg cagccctcgt tgcgggaccg gccgttttcg gacatgtccc
     7621 tgcgctggcc gcgcagcttg cgttcctaca ggacgtccac ccccacggct tcgctcacgt
     7681 gccgcacgcc gtcggcttgc agcaatcgcg gcagctcgcg gttgatggca gccgccacgc
     7741 cggggccttc gtacatgagc gccgtcatca gttgcaccag cgatgcgccg aggcggatct
     7801 tccggtaggc gtccgcggcc gtgaacacgc cgcccgagcc gatgaggcgg tagcgcgaac
     7861 ggtccatttg ccggtagagc tcggagaggg tccggtccgc cgccgccatc gccggcggcc
     7921 ccgagaccgc gccgggcatc gccccgagtt gccatgccgg cgttctcagg ccgccgggct
     7981 tgccgggcgg caggttgacc gagaagccgg tgaccgaacg cgccggtttc accgtcgcga
     8041 gaaaggtttc cagctccgcc ggcgattgaa acggcgccac cttcaggaac agcggcctgg
     8101 ccaccccccg gccatcgagc gaatcgagtc cctgcagcag ttgcgcgagt cgcgccggct
     8161 cgtggaagaa accgcgcccc tcgcgcgtgt tggggcagct caggttcagg cacaggtaat
     8221 ccgcgacggg ctgcaggcgc cgcaccgact gcaggtagtc ggcgatgacc gcatcatccg
     8281 gctcggcggc cgcggccgca ccgcggttgg tgctgacgat gttgatgccc agcggtgcgc
     8341 aggcacgcaa gccggccagc cgctgtgcca ctcgttccgc gccatcgttg ggcaagccgt
     8401 aattgacgac gatgccgcgg tcctgcggaa tgcggaacag gcgcggcttg gggttgcccg
     8461 ccgacggctc ggccgagatg gaccccacct cgacgtggcc gaaaccgagc gccgccagga
     8521 acggcaccgc gcgcgcgctc ttgtcgaagc ccgcggccag gcccagcggc gttcgaaagc
     8581 gcaggccgcc gatcgccacc tcgagccgct cactgtccgc acagtgccgc cgcgcgacgc
     8641 tggaacacag ccagcgcgac gagctcgcca gttcggcggc ccggatggca aggcagtgca
     8701 tggtttccgc ctcggcgcgg aacagcagcg gccgcagcag tgggcgatac aggttcatga
     8761 cccgattgtc gttcggccgg tgtgccgttg cggcatgggc ccggggccta caatttgccc
     8821 tttgccccaa gccccaccgg aggctcgcca tgcagttctc gccgtccatc ttcaaagcct
     8881 atgacattcg tggggtcgtg ccgtcgacgg tgaacgagga gatggcgcag gcgctggggt
     8941 gggcgttcgg aaagcgggcg caggccgaag gggaaaaaac cgtcgcggtc gggcgcgacg
     9001 ggcggctcag cgggcccgcc ctggtcgcgg ccctggtgcg ggggctcgcc gcctccggcg
     9061 tcgacgtgat cgacatcggc ccggccacca cgcccatgct ctactttgcc gcgagcacct
     9121 tgtgttccag cggcgtccag gtgaccggca gccacaatcc caaggattac aacggcttca
     9181 agatggtgat cgccggcagg gccatccatg gccaggagat tcaagccttg cggccattga
     9241 tgcaggcgtg gagccccgaa gcagcggcaa cggccgggcc ggggcgcgtg cgcaacatca
     9301 acatcctcgc cccgtaccgg gaccgcatcg tgcaggacgc gaagctggcc cgtcccatga
     9361 agatcgtggt cgattcgggc aacggtatcg ccggcgcgtc ggcgcccgga attttccgtg
     9421 cgctgggctg cgaggtggtg gaactcttca gcgaggtcga cgggaccttt cccaaccacc
     9481 atcccgatcc cagcaagccg gagaacctga aggacctgat cgccgcgatc cgcgccagcg
     9541 gcgcggaact cggcctcgct ttcgatggcg acggcgaccg gctgggcatc gtcacgcgcg
     9601 gcggcaacaa catctacccg gaccgccaga tgatgctgtt cgcccgcgac gtgctgacgc
     9661 gtgtgcccgg cggcaccatc ctgttcgacg tgaagtgcac gcagcgtctg gccccggcca
     9721 tccgcgaggc cggcggcgtt ccgctgatgt acaagaccgg ccattcgctg atcaaggcca
     9781 agatgaagga agtggcgtcg ccgctgggcg gggagatgag cggccacatt tttttcaagg
     9841 acc
//
LOCUS       DASWIY010000435        15969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1806442, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000435 DASWIY010000000
VERSION     DASWIY010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 15969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15969
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806442"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..995
                     /locus_tag="VGA59_15475"
     CDS             114..995
                     /locus_tag="VGA59_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX9721118.1"
                     /translation="MSTRAPGTTPYYFVPQPSRHPAFAAFGLFWVIFGASQWINGAGW
                     GKYLLFFGFAWLLGVLFQWFRDTIRESEGGRYGHKIDLSFRWSMSWFIFSEVMFFGAF
                     FTALWWMRAHSVPALGGLDNALLWPDFKAVWPTLSPGSTASPAGIVDPFQVMTPFWLP
                     TINTALLLSSGVTLTIAHHALRENHRGRTIAFMWITVLLGATFIGVQAYEYFHAYSDL
                     NLKLSSGAYGSTFFMLTGFHGFHVIVGMLMLLFITLRLMEGHFTPDRHFGFEGAAWYW
                     HFVDVVWLGLYTLVYWL"
     gene            complement(1057..1278)
                     /locus_tag="VGA59_15480"
     CDS             complement(1057..1278)
                     /locus_tag="VGA59_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEX9721119.1"
                     /translation="MKYLVLMAFVAIIGSLGSALFFMMRDGQDGRPKTSNMARALAFR
                     VGFSILLFVCILLAWKFGYIQPTGIPLTK"
     gene            1294..2022
                     /locus_tag="VGA59_15485"
     CDS             1294..2022
                     /locus_tag="VGA59_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX9721120.1"
                     /translation="MSKLRPGRFWLVTLAAVAALAVTLALGAWQLGRAHEKLALQASM
                     EERKALPPVGEQALLDARPSGDSRYRPVVLRGTWLARHTVFLDNRQMNGKTGFYVVTP
                     LRLEGSGRAVLVERGWAQRNFVNREQLPPIDTPPGIVQVRGRMAPPPAKLYEFDGAPS
                     GPIRQNLDLAQWRAETGLPLLEVGVQQTGETSDGLLRDWPDAAGGAGKNYGYAFQWWA
                     LSALIAILYVWFQFIAPRRKARHA"
     gene            2015..2695
                     /locus_tag="VGA59_15490"
     CDS             2015..2695
                     /locus_tag="VGA59_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721121.1"
                     /translation="MLDEPLGLTVHSLPAPQDVLASDAQRTVRGRWRMLGVLLVCAAP
                     VIASYLTYYVIRPEARRNYGELIEPQRPLPALKATALDGAAVALPSLKGQWLLVSVGG
                     GACDANCERHLYLQRQLRESLGPDKERVDWIWLVADGAQVREPLRPALSQATVLQVNA
                     GALAQWLAPAPGHQLSDHLYVVDPLGNWMMRFPAGLDMAGAAKAKRDLQRLLRASASW
                     DQPGRSRP"
     gene            2714..3862
                     /locus_tag="VGA59_15495"
     CDS             2714..3862
                     /locus_tag="VGA59_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX9721122.1"
                     /translation="MNPQPLYDLSPTLHLMIMSLVVALGPLSWFWLRNKKAQPARRLQ
                     ALTLLVLFLTFDLVVFGAFTRLTDSGLGCPDWPGCYGSASPSGAHSEISKAEAAMPTG
                     PVTHEKAWIEMIHRYLAMAIGVLLITLTAATWLQRRRGAPVSTWWPAATLAWICVQGA
                     FGAWTVTMKLFPAIVTLHLLGGVVLLALLCRQAVAYEHAHSGAARASIAPSDRAWLIA
                     ALGLVWLQVALGGWVSTNYAVLACTDFPTCQGSWWPSMNFNEGFELWRELGQTGAGQY
                     LSFAALTAIHYAHRLLAYAVFIVLGWLAWRLWQIAPLRRQGRWVAGLALLQLVTGLSN
                     VVLGWPIVAAVSHTGGAAALVVVLTWALCESRAAVQSRAGLKSQGLSA"
     gene            3859..4740
                     /gene="cyoE"
                     /locus_tag="VGA59_15500"
     CDS             3859..4740
                     /gene="cyoE"
                     /locus_tag="VGA59_15500"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX9721123.1"
                     /translation="MTLRLQQFYALTKPRVVQLIVFCALIGMVLAVPGLPTLADLRLG
                     LFACAGIWLVAGAAAVFNCLVEKGIDAKMRRTAWRATAKGELSDWQTLLFAGVLCALG
                     SWLLYVWVNPLTMWLTFATFVGYAVVYTVILKPLTPQNIVIGGASGAMPPVLGWAAMT
                     GDVGPEALILFLIIFLWTPPHFWALALYRVEDYRKSGLPMLPVTHGNEFTRLQIFLYT
                     LVLFAACLMPFAYGMSSWLYLVVALALSFGFSLYGFRLWRVYSDALARKTFRFSLIHL
                     SLLFATLLVDHYIFYTS"
     gene            4737..5345
                     /locus_tag="VGA59_15505"
     CDS             4737..5345
                     /locus_tag="VGA59_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX9721124.1"
                     /translation="MNRRNLIASLAAGALAAGTASLLGACSESKPQFKAIDLTGADYA
                     GDFQLTDHNGQPRSLKDFRGKLVVVFFGYTQCPDVCPTTMAELAEAKKLLGPDGDKVQ
                     GLFITVDPERDTPELLKAYMGNFDPGFLALRGTPEQLAAVAKEFKVYYKKAEGKTPTS
                     YTMDHSAASYVYDTQGRLRLYTRYGSGAQALASDLQLLLKHG"
     gene            5347..6162
                     /locus_tag="VGA59_15510"
     CDS             5347..6162
                     /locus_tag="VGA59_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX9721125.1"
                     /translation="MVRHLLGRKGAARIALIPPEVLEALNAGLVPTVNLNEFLALDLS
                     RLARSVARDVGLAPEDERIADTLAMLDAFRPVKRHEHVARALYDLTEPRADRDAIAHR
                     LATHPSDIARSWATQWIAFSPMALSEKLHSVRRFAADPHFGVREMAWMAVRDEVAGAV
                     DDAVALLQPWVRDPDENIRRFASELTRPRGVWCAQIDALKAQPWRALALLEPLKADPS
                     HYVRNSVANWLNDASKSQPEWVDEVCRRWLAESGVPATQYIVRRACRSLSSRA"
     gene            complement(6220..7230)
                     /locus_tag="VGA59_15515"
     CDS             complement(6220..7230)
                     /locus_tag="VGA59_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9721126.1"
                     /translation="MSNHPVRRILLLLALAAVSAGAFAQKTVKSKAAAMPAAPWPTKP
                     LRIMVGFPGGSTPDLVARTIAEPLSKALGQPVIVENRPGAAGNIAADIVAKSTDNHTI
                     GVMINGNMTIAKLLNPATPFDPLKDLAPISLIGTAPLLLTAPANAPGNNAKEFFAAAR
                     SAGNKWSYGSPGVGTVGHLGMELLKTQASIDPVHVPYPGNPQVINAMIGGQLQLSLQP
                     PGLAAAQIRAGKLKAVGVTSSGRSPLVPEFPSLAEGGVKGFQLEIWTAAAGPKSLPQP
                     IVTRLSSLIGEIARTPEVRQKLFQQGWQVAGTSSEGLANRIKADTALLGGVIAMRGIK
                     AE"
     gene            complement(7313..9052)
                     /locus_tag="VGA59_15520"
     CDS             complement(7313..9052)
                     /locus_tag="VGA59_15520"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX9721127.1"
                     /translation="MRRIIYACWAALLWLVGPGAAFGQDALPAPRVAAPPSVIVLAPQ
                     HPVLSLDGRSRYWLDPAEVRTVDQLEAAGDSLPWVPREGGRSYNIDGKALWLQFDTVS
                     KGEQRWYVQLASSGIDRVQLFYRGADGRWVEQEAGDTKPVSQWPLPGRFPTFELATGA
                     SSPVRHWLRIEHARVDFASPIAIYAQSSLLAAREREQFLLGAYFGLAALIALVAAANA
                     AAYRDRSFGVYAFYVAALAAGQLAYLGVGAQHLWDQSLKWNETATFLLPGISSAAALW
                     FARTVTEPARFSKTLDLAVWSLIAALLSAVALDTFLTSRNSFALVMLLILVAMGVVVG
                     LIALVWTQGDDPYIRLVALGFLPVLVMALFPVARGLNLIPASALTRYALSIGAVIEMP
                     ILFYALSLRGSRRREAQVRAAALSRNDALTGLAHSRTLLQRLEGAITRARSLKHACVL
                     MAVKISNFEAITAQYGRETADRVLVVTASLLRHAITDIDMAARVGEHEFALLLEGPTT
                     KENAMSRAQQVVASGLRSSQALPAGTALKFHVALALLPDKDLDAARSMKWLLDSVAAM
                     PAEARKLIRPLNF"
     gene            complement(9153..10085)
                     /gene="rpoH"
                     /locus_tag="VGA59_15525"
     CDS             complement(9153..10085)
                     /gene="rpoH"
                     /locus_tag="VGA59_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX9721128.1"
                     /translation="MSTTMGAPRTELAVANPWAVVPSLGNLDAYISAVNRLPMLTPQE
                     EQEFARKFKEHNDIEAAGKLVLSHLRLVVSVSRKYLGYGLPHGDLIQEGNIGLMKAVK
                     RFDPDQGVRLVSYALHWIRAEIHEYILKNWRMVKVATTKAQRKLFFNLRSMKQGFKDE
                     ADAQTHRDSLTDAQIDVMARGLNVKREEVIEMEARMAGGDVLLDPAPSDDGEESFGPI
                     AYLSDASHEPTAVMESRQRDSLATDGVAAALAQLDARSRRIVEERWLKVNDDGSGGLT
                     LHDLAAEYGVSAERIRQIESAAMKKMKKALAEYA"
     gene            10291..10749
                     /locus_tag="VGA59_15530"
     CDS             10291..10749
                     /locus_tag="VGA59_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721129.1"
                     /translation="MRRMISRVFSRSGEPAPPAAGHSALSSELSTSDIETYYLGVIVD
                     CLRRMLLPLDSFEVRVRPSGRSPAGLSAFAGYVRILKWDTVCTPILLQNLPVIDARVR
                     KVVSASVILEGTHFAGLWFQAGSDVEGAPKSLLGLPCELVHQPRSTAADA"
     gene            10809..11156
                     /gene="cutA"
                     /locus_tag="VGA59_15535"
     CDS             10809..11156
                     /gene="cutA"
                     /locus_tag="VGA59_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX9721130.1"
                     /translation="MQVREELDILSVTTTVDSPAAARTLAHEILAQRLGACVQIDQGV
                     TSLYHWQGALREEPEVRLAIKTLPACAAALQALFARYHPYEVPQFLAQHMRASPAYAD
                     WVRAETLVPKAEP"
     gene            complement(11173..12261)
                     /gene="selD"
                     /locus_tag="VGA59_15540"
     CDS             complement(11173..12261)
                     /gene="selD"
                     /locus_tag="VGA59_15540"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX9721131.1"
                     /translation="MSAKGEGPRVRGSTSAEPRLTSLSHGGGCGCKIAPGVLSEILKD
                     TARLPVPPELLVGIETSDDAAVYRLNDEQALIATTDFFMPIVDDPFDFGRIAATNAIS
                     DVYAMGGRPIMALALVGMPVNVLSTTTIGLILAGGQSVCREAGIPVAGGHTIDSVEPI
                     YGLVALGLVHPDRVRRNADARPGDMLVLGKPLGVGVLSAALKKNALDAAGYARLIATT
                     TQLNTPGPELAALEGVHALTDVTGFGLAGHALELARGAGLRVRIEWSGVPLLPGAREL
                     AQQGMVTGASARNWAGYSADVELPAEFGPVDRALLSDPQTSGGLLVSCAPQAVDEVLA
                     LFRRQGFAGAAVIGEVGPSGSPPRLVVA"
     gene            complement(12258..13331)
                     /gene="ftsY"
                     /locus_tag="VGA59_15545"
     CDS             complement(12258..13331)
                     /gene="ftsY"
                     /locus_tag="VGA59_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX9721132.1"
                     /translation="MFSFFKKKPAPPPATPLPAVPAPAQGGSLIGSALVQPIEVPASA
                     PVAPERQRWMEKLSAGLRKTGSSISLVFTGAQIDDQLYEELESALLMADTGVKATQYL
                     LDEVKRRVQDSGATRPVQVKNILADSLTQLLRPLEKALAIGEHRPTVIMVAGVNGAGK
                     TTSIGKLTWHLAHEGASVLLAAADTFRAAAREQLAVWADRNTVEIVSQEGGDPAAVSF
                     DAVTAGKARGKDVVLVDTAGRLQTQLHLMEELRKIKRSIQKAGPAPSAANMESSLPPH
                     EVLLVIDGNTGQNALAQVRAFDDALQLTGLIVTKLDGTAKGGVLAAIAQEKPVPVYFI
                     GVGEKLEDLETFNAREFALALLS"
     gene            13361..14764
                     /locus_tag="VGA59_15550"
     CDS             13361..14764
                     /locus_tag="VGA59_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9721133.1"
                     /translation="MKHPMRRFCWSISLLFPLWLAPPALAQTAPRVEQFKLDNGLTVI
                     VKPDRRAPTAVHMLWVRAGALEEVDGTSGVAHVLEHMMFKGTREVKAGEFSRRVAALG
                     GRENAFTNRDTTGYYQQIPAGKLEDVMKLEADRFANNQWPDDEFKREIEVVKEERRQR
                     TEDSPRSLMWEALSATVFQASPYRRPVVGWMSDLEAMTAQDVREFHRSWYVPANAAVV
                     VAGDVEPAQVRRLAERYYGRIRARAVPARKPRSEPQQVGLRRIEFKAPADQAYVALAF
                     KVPQLTSLEPGPQNDDALALTVLAAVLDGYSGARLDRALTQGSDRLADSAGAGNGLWG
                     RGPQLFTLDGVPARGKTAEQLEAALRAEVAKVARDGVSEAELNRVKTQWIAGEVYKLD
                     SVMSQARELGGEWVQGLPLDAGERLIERLRGITAEQVKSVAAKYFGDDQLTAGILRPQ
                     PVDPNRKPRTPPPGLRH"
     gene            14780..>15969
                     /locus_tag="VGA59_15555"
     CDS             14780..>15969
                     /locus_tag="VGA59_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9721134.1"
                     /translation="MILASKPIHAAVAAAGAALLLGWGQAIAAIPIQRWTQPNGAAVF
                     LVESPTIPMVDVHIDFDAGSRRDPADKAGLARVTAGMTSKGITAASPAIGGPYDAALD
                     ENQLSEAWADLGAGLGTGASTDRMSFSLRSLSYPDLLPKAAQLAARQLGDPAFPDAVW
                     QRERQRLAASLREAKTRPATVAGQAYAPAVYGDHPYGYEMTEESLERIAVQDMMQVYR
                     RLIQPCRAKVSIVGAVTRAQADALVTTLLSRLPAGQAGCEPLPPVAEVPPLTAPVVRE
                     IPFASAQAHVLIGQPGYKRNDPDFFALVLGNYILGGGGFVSRLSQEVREKRGLSYSVY
                     SYFSPGLHAGAFTLGLQTRPDQAAQAVQVSREVLARFVTDGPTEAELKAAKDNLIGGF
                     PLLI"
BASE COUNT         2495 a         5383 c         5364 g         2727 t
ORIGIN      
        1 gttccatatc atcgccgtgg ccctggtggg cgtggccttg ttcgtggccg ggctgatcgc
       61 cctggtcagc tgggtggcga aataagtgca agaaatatgg accaggagtt gagatgagta
      121 cacgcgcacc cggcaccacg ccctattact tcgtgcccca gccctcgcgc cacccggcgt
      181 tcgccgcatt cgggctgttc tgggtcatct tcggcgcgtc ccagtggatc aacggcgccg
      241 gctggggcaa gtacctcctg ttcttcggct tcgcctggct gctgggcgtg ctgttccagt
      301 ggttccggga cacgatccgc gagagcgaag gcgggcgtta cggccacaag atcgacctct
      361 cgttccgctg gagcatgagc tggttcattt tttcggaggt gatgttcttc ggcgccttct
      421 tcaccgcgct gtggtggatg cgcgcgcatt cggttccggc gctcggcggc ctcgacaacg
      481 cgctgctgtg gcccgacttc aaggcagtct ggcccacgct gtcccccggc agcaccgcat
      541 cgcccgccgg catcgtggat ccgttccagg tgatgacgcc gttctggctg ccgacaatca
      601 acaccgcgct gctgctgtct tcgggtgtca cgctgaccat cgcgcaccat gccctgcgcg
      661 agaaccaccg cggccgcacg atcgcgttca tgtggatcac cgtgctgctt ggcgccacct
      721 tcatcggggt gcaggcctac gagtacttcc acgcctacag cgacttgaac ctcaagctca
      781 gctccggcgc ctacggctcg actttcttca tgctcaccgg ttttcacggc ttccacgtga
      841 tcgtcggcat gctgatgctg ctgttcatca ccttgcggct gatggaaggg cacttcacgc
      901 ccgatcgcca cttcggtttc gagggggcgg cctggtactg gcacttcgtg gacgtggtct
      961 ggctggggct ttacacgctg gtttattggc tatagcttcg aagggctgcg cgaggagtaa
     1021 acaaggcgcc ccgaagggcg ccttgtactt tgtgatttac ttcgtcaggg gtatccccgt
     1081 cggctggata tagccgaact tccacgccag caggatgcac acgaacagca ggatcgaaaa
     1141 gcccacgcgg aacgccagcg cgcgcgccat gttgctggtc ttgggcctgc cgtcctggcc
     1201 gtccctcatc atgaagaaaa gcgccgaccc cagactgccg atgatggcca cgaacgccat
     1261 gagcacgagg tatttcatgg cagcgattat cccgtgagca agctccgccc gggtcgcttc
     1321 tggctggtca ccctcgcggc cgtggcggca ttggcggtca cgctcgcgct gggcgcctgg
     1381 caattgggcc gcgcgcatga aaagctcgcg ctgcaggcct cgatggagga gcgcaaggcg
     1441 ctgccgcccg tgggcgagca agccctgctc gatgccaggc cgtcgggcga tagcaggtat
     1501 cggccggtgg tgctgcgggg tacctggctc gcgcggcaca cggtttttct ggacaacagg
     1561 caaatgaacg gcaagaccgg cttctacgtg gtgacgccgc tgcggctgga aggcagcggg
     1621 cgggcggtgc tggtcgaacg aggctgggcc cagcgcaatt tcgtgaatcg cgagcagctg
     1681 ccccccatag acaccccgcc gggcatcgtc caggtgcgcg ggcgcatggc cccgcctccc
     1741 gcgaagctat acgaattcga cggcgcgccg agcggcccca tacggcaaaa tctcgacttg
     1801 gcacaatggc gtgccgaaac cggactgccg ttgctggagg tcggagtgca gcagaccgga
     1861 gaaacttcag atgggctgct gcgcgactgg cccgacgcag ccggcggcgc cggaaaaaac
     1921 tacggctatg ccttccagtg gtgggccctg agcgccctga ttgcaatcct ctatgtctgg
     1981 ttccagttca tcgcccctcg ccggaaggcc cgccatgctt gacgagccgc tgggattgac
     2041 cgtgcattca ttgcccgcgc cgcaggacgt gctggcgagc gatgcccagc gcaccgtgcg
     2101 cggccgctgg cgcatgctgg gcgtgctgct ggtctgcgct gctcccgtga tcgcttctta
     2161 cctcacctat tacgtcatcc gaccggaggc gcggcgcaac tacggcgaac tgatcgaacc
     2221 ccagcgtcca ttgcccgccc tgaaggcgac agctttggac ggcgcggcgg tggcgctgcc
     2281 atcgctcaag gggcagtggc tcctggtcag cgtgggtggc ggggcctgcg atgcgaactg
     2341 cgaacgccac ctctacctgc agcgccagct gcgcgagagc ctgggcccgg acaaggagcg
     2401 cgtcgactgg atctggctgg tggccgacgg cgcccaggtg cgtgagccgc tgcgccccgc
     2461 cttgtcgcaa gccaccgtcc tgcaggtgaa tgccggcgcg ctggcgcaat ggctggcgcc
     2521 ggcaccgggc catcagcttt ccgaccatct gtatgtggtc gacccgttgg ggaactggat
     2581 gatgcgtttt cccgcgggcc tcgacatggc cggtgctgcc aaggccaagc gcgacctgca
     2641 gaggctgttg cgcgcctcgg cctcgtggga ccagccgggc cgaagcagac cttgatttga
     2701 cctggacccg aacatgaacc cgcagcccct gtacgacctc tcgcccacct tgcacctcat
     2761 gatcatgagc ctggtggtgg cgctcgggcc gctgtcctgg ttctggctgc gcaacaagaa
     2821 ggcgcaaccg gcccgccggc tgcaagcact gacgctgctg gtgctgttcc tcaccttcga
     2881 cctggtggtg ttcggcgcct tcacgcggct gaccgattcc ggcctgggtt gccccgactg
     2941 gcccggctgc tacggcagcg ccagccccag cggcgcgcat tccgagatca gcaaggccga
     3001 ggcggccatg ccgaccgggc cggtgacgca cgagaaagcc tggatcgaaa tgatccatcg
     3061 ctacctggcc atggccatcg gcgtgctgct catcactttg acggccgcga cctggctgca
     3121 gcgcaggcgc ggcgccccgg tcagcacctg gtggccggcg gcgaccctgg cctggatctg
     3181 cgtgcagggc gcgttcggtg cctggaccgt cacgatgaag ctgtttccgg cgatcgtcac
     3241 gctgcacctg ctggggggcg tcgttctgct ggctttgctg tgccgccagg cagtagccta
     3301 cgagcacgcg cacagcggcg ccgcgcgcgc gtcgatcgcg ccatccgacc gggcctggct
     3361 gatcgcggcg ctgggcctgg tgtggctgca ggtggcgttg ggcggctggg tcagcaccaa
     3421 ctacgccgtg ctggcctgta ccgatttccc cacctgccag ggcagctggt ggcccagcat
     3481 gaatttcaat gaaggcttcg agctgtggcg cgagctgggc cagaccggcg ccggccaata
     3541 cctcagtttc gccgcgctca cggccatcca ctatgcccat cggctgctgg cctatgcggt
     3601 gttcatcgtg ctgggctggc tggcctggcg gctgtggcag atcgcgccat tgcggcgcca
     3661 gggccggtgg gttgccggcc tggcgctgtt gcagctggtc acgggcctgt cgaacgtggt
     3721 gctgggttgg ccgatcgtgg cggccgtctc gcataccggc ggtgccgccg cgctggtcgt
     3781 ggtgctcacc tgggcgctgt gcgaaagccg cgccgcagtc cagtcgcggg cgggcttgaa
     3841 gtctcaaggc ctgtcggcat gacactgaga ctgcaacagt tctatgcgtt gaccaagccg
     3901 cgcgttgtgc agttgatcgt gttctgcgct ctgatcggca tggtgctggc ggtgcccggg
     3961 ctgcccacgc tcgccgacct gaggctgggc ctcttcgcct gcgccggcat ctggctggtg
     4021 gccggcgcgg ctgccgtctt caattgcctg gtcgagaagg ggatagacgc caagatgagg
     4081 cgcacggcct ggcgggctac cgccaaaggc gagttgagcg actggcaaac ccttctcttc
     4141 gcgggcgtgc tgtgcgccct gggttcctgg ctgctctatg tctgggtcaa cccgctgacc
     4201 atgtggctca cgttcgcgac tttcgtcggc tatgccgtgg tctacacggt catcctcaag
     4261 ccgctgacgc cgcagaacat cgtgatcggc ggcgcgtcgg gcgcaatgcc gcccgtgctg
     4321 ggctgggccg cgatgacggg cgacgtcggg cccgaggcgc tgatcctctt tctcatcatc
     4381 ttcctgtgga caccgccgca cttctgggcc ctggcgctgt accgggtgga ggactaccgc
     4441 aagtcgggcc tgccgatgct cccggtcacg cacggcaacg agttcacgcg cctgcagatc
     4501 ttcctgtaca cgctggtgct gttcgccgcg tgcctgatgc ccttcgccta cggcatgagt
     4561 tcgtggctgt acctggtggt cgcgctggcg ctgagttttg gtttttcgct gtatggtttc
     4621 cggctctggc gcgtctactc ggacgcgctc gcgcgcaaga ccttccgctt ctccttgatc
     4681 cacctgagct tgctgttcgc gacgttgctg gtggaccatt acatcttcta cacatcatga
     4741 accgccgaaa cctcattgca tcgctggccg cgggtgcgct cgcggctggt accgccagcc
     4801 tgctgggggc ctgttcggaa tcgaaaccgc agttcaaggc catcgacttg accggcgccg
     4861 actacgccgg cgatttccag cttaccgacc acaacggcca gccgcgcagc ctgaaggact
     4921 tccgcggcaa gctggtggtg gtgttcttcg gctataccca gtgcccggac gtctgcccga
     4981 ccacgatggc cgaattggcc gaggccaaga agctgctggg cccggacggc gacaaggtgc
     5041 aggggctgtt catcaccgtc gacccggagc gcgacacacc cgaacttctc aaggcgtaca
     5101 tgggcaactt cgaccccggc ttcctggcgc tgcgcggaac gcccgagcag ctggccgccg
     5161 tggccaagga gttcaaggtg tactacaaga aggccgaggg caagacgccc accagctaca
     5221 ccatggacca ctccgccgcg agctacgtct acgataccca ggggcggttg cgcctgtaca
     5281 cccgctacgg cagcggcgcc caggcgctgg cctcggacct gcagttgctg ctcaagcacg
     5341 gctgaaatgg tccggcacct gctcggtcgc aagggcgccg cgcgaatcgc gctcatcccg
     5401 cccgaggtgc tggaggcctt gaacgcggga ctggtgccca ccgtcaacct caacgagttc
     5461 ctggccctcg acctgtcgcg gctggcccgc agcgtggcgc gcgacgtcgg cctcgcgccc
     5521 gaggacgagc gcatcgcgga cacgctggcg atgctggacg cgttcaggcc cgtgaaacga
     5581 cacgagcacg tcgcccgcgc gctgtacgac ctgaccgagc cgagagcgga ccgcgatgcg
     5641 atcgcccatc gcctggccac ccaccccagc gacattgcac ggtcctgggc gacgcaatgg
     5701 atcgcgttct cgccgatggc gctgtccgaa aaactccatt cggtgcgccg cttcgccgcc
     5761 gatccccatt tcggcgtgcg cgagatggcc tggatggccg tgcgcgatga ggtcgcaggc
     5821 gcggtcgacg atgccgtggc cctgctgcag ccgtgggtcc gcgacccgga cgagaatatc
     5881 cggcgctttg ccagcgaact cacccgtcct cgcggcgtgt ggtgcgcgca gatcgacgcg
     5941 ctgaaagcgc agccctggcg tgcgctcgcc ctgctcgagc cgctgaaggc cgaccccagc
     6001 cactacgtgc gcaactcggt ggccaactgg ctcaacgacg cgagcaagtc gcaacccgaa
     6061 tgggtcgacg aggtgtgccg gcgctggctg gccgaatcgg gggtccctgc aacccaatac
     6121 atcgtcaggc gcgcgtgccg aagcttgtca tcccgggcgt gacccgggat ccaggggtca
     6181 tggattgcgg ggccgagcct ggtcgagccc gcaatgacat tactccgcct tgatgccccg
     6241 catcgcgatc acgccgccca gcagcgccgt gtcggccttg atgcggttgg ccagtccttc
     6301 ggaagaagtg ccggcgacct gccagccttg ctggaacagt ttttgccgca cttcaggcgt
     6361 gcgcgcaatt tcgccgatca gcgacgacag ccgggtcacg atgggttgcg gcagggactt
     6421 cggccccgcg gctgccgtcc agatctccag ctggaagccc ttgaccccgc cctcggcgag
     6481 gctggggaat tccgggacca gggggctgcg cccggaggag gtcacgccta cggccttgag
     6541 cttgcccgcg cggatctggg cggcggcaag gccgggcggc tgcagcgaca gctggagctg
     6601 gccgccgatc atggcattga tgacctgcgg gttgccgggg tagggtacat gcaccgggtc
     6661 gatgctggct tgggtcttga gcagctccat accgagatgg cccaccgtgc cgacgcccgg
     6721 cgaaccatag ctccacttgt tgcccgcact gcgagccgcc gcgaaaaact ccttggcgtt
     6781 gttccccggc gcgttggccg gcgccgtgag cagcagcggc gcggtgccga tcaggctgat
     6841 cggcgcgaga tccttcagcg gatcgaatgg cgtggccggg ttcagcagct tggcaatggt
     6901 catgttgccg ttgatcatca cgccgatggt gtggttgtcg gttgacttgg caacgatgtc
     6961 ggccgcgatg ttgcctgcag cgccgggccg gttttccacg atcacgggtt gccccagcgc
     7021 tttcgacagg ggctcggcga tggtgcgggc caccaggtcg ggcgtggagc cgccagggaa
     7081 accgaccatg atgcgcagcg gcttggtcgg ccaaggggcg gcgggcatgg ccgcggcttt
     7141 ggatttgacc gttttctgcg cgaacgcgcc cgccgagacg gcggccagcg cgagcaagag
     7201 gagaattcgg cgcacagggt gattcgacat gctgggtact ttcttggaat ggcgggcccc
     7261 aaccgggccc aaccgcaatc gtagcgcctg ggcgcgggag cttcggtgcc gttcagaagt
     7321 tcagcgggcg gatcagcttg cgcgcctcgg ccggcatggc cgccacgcta tcgagcagcc
     7381 acttcatgct gcgcgcggcg tccaggtcct tgtccggcaa cagcgccagc gcgacgtgga
     7441 acttgagcgc ggtgccggca ggcagggcct gcgaggacct caggccgctg gccacgacct
     7501 gctgggcgcg gctcatcgcg ttctccttcg tcgtcgggcc ttccagcagg agcgcgaact
     7561 catgctcgcc cacgcgcgcg gccatgtcga tgtcggtgat ggcgtggcgc aacagggagg
     7621 ccgtgacgac caggacgcgg tcggccgtct cgcgcccgta ttgggcggtg atcgcctcga
     7681 agttcgatat tttcacggcc atcaggacgc aggcgtgctt gaggctgcga gcgcgcgtga
     7741 tcgcgccctc caggcgctgc agcagggtgc gggagtgggc cagcccggtg agcgcgtcgt
     7801 tgcgcgacag ggcggcggcg cgcacctgcg cttcgcggcg gcggctgccg cgcaggctca
     7861 gggcatagaa caggatcggc atttcgatca ccgccccgat cgacaatgca tagcgcgtca
     7921 gtgcgctggc ggggatcagg ttcaggccgc gcgcgaccgg gaacagcgcc atcaccagca
     7981 ccggcaggaa cccaagcgcg accaggcgga tgtagggatc gtcgccttgc gtccacacga
     8041 gcgcgatcag ccccaccacg acccccatcg cgaccaggat cagcaacatc accagcgcga
     8101 acgagttgcg cgaagtgagg aacgtatcca gcgccacggc cgatagcagc gccgcgatca
     8161 ggctccatac cgccaggtcc agcgttttgg aaaagcgcgc gggctcggtg accgtacgcg
     8221 cgaaccacag cgcggccgcc gaggaaattc ccggcagcag gaaagtggcg gtttcgttcc
     8281 acttgagcga ttggtcccat aaatgctgcg cgccgacgcc caggtaggcg agctggcccg
     8341 cggcgagcgc ggccacgtag aaggcgtaga cgccgaagct gcggtcgcgg taggccgcgg
     8401 cattggcggc ggcgaccagt gcgatcagcg ccgccaggcc gaaataggcc cccagcagga
     8461 actgttcgcg ttcgcgcgcg gccagcagcg acgactgcgc ataaatggcg atggggctcg
     8521 cgaagtccac ccgcgcgtgc tcgattcgca gccagtggcg caccgggctg gacgccccgg
     8581 tcgccaactc gaaagtgggg aaccgcccgg gcagcggcca ttgcgacacg ggtttggtgt
     8641 cgccggcctc ctgctccacc cagcgcccgt cggcgccgcg gtagaacagc tgcacccggt
     8701 cgatcccgga agaggcgagc tggacgtacc agcgctgctc gcccttgctc acggtgtcga
     8761 actggagcca cagcgccttg ccgtcgatgt tgtagctgcg gccgccctcg cgcgggaccc
     8821 agggcaggga gtcgccggcc gcctcgagct ggtccacggt gcgaacttcc gccgggtcca
     8881 gccagtagcg gctgcgcccg tcgagcgaca gcacgggatg ttggggcgcg agaacgatga
     8941 cggacggggg ggccgcgacc cgcggcgcgg gcagggcgtc ctggccgaag gcggcgcccg
     9001 ggcccaccag ccagagcagc gccgcccagc atgcgtaaat gattctcctc actcagccat
     9061 tgttgcagca ttcgcgggcc gaccgaccaa gaaagaggcc cgggcgtagc cgggcctcat
     9121 gaatcacctg tcacggccgg cgccgccgcc ctttacgcat attcggcgag cgccttcttc
     9181 attttcttca tggcggcgga ctcgatctgg cggatccgct ctgcgctgac gccgtattcg
     9241 gccgccaggt cgtgcagcgt caatccgccc gagccgtcgt cgttgacctt gagccagcgc
     9301 tcttcgacga tgcgccggct gcgcgcatcg agctgcgcca gcgcggcggc gaccccgtcg
     9361 gtggccaggc tgtcgcgctg gcgcgattcc atgaccgccg tcggttcatg gctggcatcg
     9421 gagaggtacg cgatcggccc gaaagactcc tcgccgtcgt cgctcggcgc cggatccagc
     9481 agcacgtcgc cgccggccat tcgcgcttcc atttcgatga cttcctcgcg cttgacgttg
     9541 agcccccgcg ccatcacgtc gatctgggcg tcggtcaggc tgtcgcggtg ggtctgcgca
     9601 tcggcttcgt ccttgaagcc ctgcttcatc gagcgcaggt tgaagaacag cttgcgctgg
     9661 gccttggtgg tcgccacctt gaccatgcgc cagttcttca ggatgtactc gtggatttcg
     9721 gccctgatcc agtgcagggc atagctcacc agccgcacgc cctggtccgg atcgaagcgc
     9781 ttcaccgctt tcatgaggcc gatattgcct tcctggatca ggtcgccatg cggcaggcca
     9841 tagcccaggt atttgcgcga taccgacacc accagccgca gatgggagag caccagcttc
     9901 ccggccgcct cgatatcgtt gtgttccttg aatttccggg cgaactcctg ctcctcctgg
     9961 ggagtgagca tcggcagccg gttcactgcc gagatgtaag cgtccaggtt gcccagggac
    10021 ggaacgaccg cccacggatt ggctacggcc agttccgtgc gaggggctcc catagtcgtg
    10081 gacatgccag aaactccttt catgtttgag tcttatatta gcactctctt tgagcgagtg
    10141 ctaaaaggca aagttcagta cagagcatac agaaatcagg gtattcccgc gcatcaaagg
    10201 gtgttcctga ttgcgccgtg tgaccgcttt cgataccatc ctgtacacat tttgaaacaa
    10261 gcgcggtcca cgcgcacagc aggaggcagc atgagacgga tgatcagccg ggtgttttcc
    10321 cggagcggcg agcccgcgcc accggctgcc ggccattcgg cgctctcctc cgagctgagt
    10381 accagcgata tcgaaacata ctacttgggc gttatcgtcg actgcctgcg tcgcatgttg
    10441 ttgccactgg acagcttcga agtccgcgtg aggccctcgg gcaggtcgcc ggccggcctg
    10501 agcgcctttg ccggctacgt ccgcattctc aaatgggaca cggtctgtac gcccatcctg
    10561 ctgcagaacc tgccggtgat cgacgcccgg gtccgcaagg tcgtcagtgc ctcggtcatc
    10621 ctggaaggga ctcacttcgc cggcctttgg ttccaggccg gcagcgacgt cgaaggcgcg
    10681 cccaagtcgc tgctcggcct gccctgcgag ctggttcacc agcctcgcag cacggcagcg
    10741 gacgcttgac gcttcaaagc gaagcccgcg cttgcgcaag aatggagccg attcgaaagg
    10801 cccgttccat gcaagtccgt gaagaactcg acatcctgag tgtcaccacc acagtggact
    10861 ctccggccgc cgccaggacg cttgcccacg agatcctggc gcaacggctg ggcgcctgcg
    10921 ttcagatcga ccagggcgtc acctcgctgt accactggca aggcgcgctg cgcgaggagc
    10981 cggaagtgcg gctggcgatc aagaccctgc cggcttgcgc ggccgcgctc caggcgctgt
    11041 tcgcccggta ccacccgtac gaggtgccgc agttcctggc tcagcacatg cgcgcaagtc
    11101 ctgcctacgc ggactgggtc cgcgccgaga cgctggttcc caaggctgag ccgtgaggcg
    11161 ggccctggtt ctctaggcca cgaccagcct gggcggggag ccgcttggtc cgacctcccc
    11221 gatcaccgcc gcgccggcga agccctgacg gcggaacagc gccagcacct cgtcgacggc
    11281 ttgaggcgca cacgacacca gcaggccgcc gctggtctgc gggtccgaca gcagcgcccg
    11341 gtccaccggt ccgaactccg cgggcagctc gacgtcggcg ctgtagccgg cccagttccg
    11401 tgcggaagcg cccgtgacca tgccctgctg ggccagttcg cgcgcgcccg gcagcaatgg
    11461 aacgccggac cattcgatgc gcacccgcag cccggccccg cgcgccagct cgagggcatg
    11521 gccggcgagg ccgaagcccg tcacgtcggt cagcgcgtgc accccttcca gcgcggcgag
    11581 ctccggcccg ggggtgttga gctgcgtggt ggtggcgatc aaccgggcat agccggcggc
    11641 gtccagcgcg ttcttcttca aggctgccga cagcacgccc accccgagcg gcttgcccag
    11701 gaccagcatg tcgccgggcc gcgcatcggc gttgcgcctg acccggtctg gatggaccag
    11761 gcccagggcg accaggccgt agatcggctc gaccgagtcg atcgtgtgac cgcccgcaac
    11821 gggaatgccg gcttcccggc acacggattg cccgcccgcg aggatcaggc cgatggtcgt
    11881 cgtactcagc acattgaccg gcatacccac caacgccagc gccatgatgg gccgcccgcc
    11941 catggcgtag acatcggaga tcgcgttggt agcggcgatg cgcccgaagt cgaacggatc
    12001 gtccacgatg ggcatgaaga agtcggtggt ggcgatcagg gcctgctcgt cgttgagccg
    12061 gtaaacggcg gcatcgtccg aggtctcgat gcccacaagc agctcgggcg gcaccggcag
    12121 gcgggccgtg tccttgagga tttccgacag cacccctggc gcgatcttgc agccgcagcc
    12181 gccgccatgg gaaagcgaag tcaggcgcgg ctccgcgctg gtcgatcccc gcacgcgggg
    12241 cccctcgccc ttcgcgctca tgatagaagc gcgagcgcga actcgcgggc attgaaggtt
    12301 tccaggtctt cgagcttctc gcccacgccg atgaagtaca ccggcaccgg cttttcctgc
    12361 gcgatggcgg ccagcacacc gcccttggcc gtgccgtcga gcttggtcac gatcaggccg
    12421 gtcagctgca gcgcgtcgtc gaacgccctg acctgcgcca gcgcgttctg gcccgtattg
    12481 ccgtcgatca ccagcagcac ctcatgcggg ggcagcgagc tttccatgtt tgcggctgaa
    12541 ggggccgggc cggctttctg gatcgagcgc ttgatcttcc tgagttcctc catcaggtgc
    12601 agctgcgtct gcagccgtcc cgccgtgtcc accagcacca cgtccttgcc gcgcgccttg
    12661 ccggccgtga cggcgtcgaa gctcacggcg gcggggtctc cgccttcctg gctcacgatc
    12721 tccaccgtgt tgcggtcggc ccagaccgcc agctgttcgc gcgccgcggc acggaaggtg
    12781 tcggccgctg ccagcagtac cgacgcgccc tcgtgcgcga ggtgccaggt gagcttgccg
    12841 atggacgtgg tcttgcccgc gccattgaca ccggcgacca tgatgaccgt cgggcggtgc
    12901 tcgccgatcg ccaacgcttt ctccagcggc cgcagcaatt gcgtgagcga gtccgccagg
    12961 atgttcttga cctggaccgg ccgggtggcg ccgctgtcct gcacccggcg cttcacttcg
    13021 tcgagcaggt actgcgtcgc cttcaccccg gtgtcggcca tcagcagcgc gctctcgagc
    13081 tcctcgtaca gctgatcgtc gatctgcgca cccgtgaaca ccagcgagat gctggaaccc
    13141 gtcttgcgca ggcccgcgct gagtttttcc atccagcgct ggcgctccgg cgcgaccggc
    13201 gcgctcgccg gcacctcgat cggctgcacc agcgcgctgc cgatcaggct gccgccttgc
    13261 gccggggccg gcacggcggg gagcggagtt gccggcgggg gtgccggctt tttcttgaag
    13321 aaactgaaca ttgatgcgct taatagaatc acaggattct atgaaacacc ccatgcggcg
    13381 cttctgctgg agcatttctc ttcttttccc gctctggttg gccccgcccg cgctcgcgca
    13441 gacggcgccc cgggtggagc agttcaaact cgacaatggg ttgacggtca tcgtcaagcc
    13501 ggaccgccgc gctccgacgg cggtccatat gttgtgggtg cgcgcgggtg ccctggaaga
    13561 ggtcgatggc accagcggcg tggcccacgt gctcgaacac atgatgttca agggcacgcg
    13621 cgaggtcaag gcgggcgagt tctcgcgccg cgttgccgca cttggcgggc gggaaaacgc
    13681 cttcaccaac cgcgacacga ccggctatta ccagcagatc ccggccggca agctcgagga
    13741 cgtcatgaag ctggaagccg accgcttcgc caacaaccag tggcccgatg acgagttcaa
    13801 gcgcgaaatc gaggtggtca aggaggagcg gcggcagcgc acggaagact cgccgcgcag
    13861 cctgatgtgg gaagccctga gcgcgacggt gttccaggct tcgccctacc ggcggcccgt
    13921 ggtcggctgg atgagcgacc tggaggcgat gacggcgcag gacgtgcgcg agttccatcg
    13981 cagctggtac gtgccggcca acgccgcggt ggttgtcgcc ggcgacgtcg agcccgcgca
    14041 ggtgcgccgt ctggccgaaa gatactacgg caggatccgc gcgcgggccg tgccggcgcg
    14101 caagccgcgc agcgagccgc agcaggtggg cctgcgccgc atcgaattca aggctcccgc
    14161 ggaccaggcg tatgtcgcgc ttgccttcaa ggtgccgcaa ctcacctcct tggagcccgg
    14221 cccgcagaac gacgacgcgc tcgccttgac cgttctggcg gccgtgctcg acggctacag
    14281 cggggcacgc ctcgaccgcg cgctgaccca gggcagcgac cgcctggccg acagtgccgg
    14341 cgccggcaac ggcctgtggg gccgtggccc gcagctcttc acgctggatg gcgtgccggc
    14401 gcgaggcaag acggccgagc agctggaagc cgcgctgcgg gccgaagtcg cgaaagtggc
    14461 gcgcgatggc gtgagcgagg ccgaactcaa tcgcgtgaag acgcagtgga tcgcgggcga
    14521 ggtatacaag ctggactcgg tgatgagcca ggcgcgcgag ctcggcggcg agtgggtgca
    14581 gggcctgccg ctggacgccg gtgagcgcct gatcgagcgc ttgcgcggca taacggccga
    14641 acaggtgaaa tcagtcgccg cgaagtactt cggggatgat cagctcaccg cgggcatcct
    14701 gcgaccgcag ccggtcgatc ccaaccgcaa accccgcacg cccccgccgg gactgcgcca
    14761 ttgaaagggc ttgcaagcca tgattcttgc cagcaaacca atccacgccg ccgtcgcggc
    14821 agcaggcgcg gccctgctgc ttggatgggg ccaggcaatc gcggcgattc caatacagcg
    14881 ctggacccag cccaatggcg ccgcggtttt cctggtggaa agtcccacca tcccgatggt
    14941 cgacgtccac atcgatttcg atgccggcag ccggcgcgac ccggctgaca aggcgggact
    15001 ggcccgggtc acggccggca tgacctccaa gggcatcacg gccgccagtc ctgccatcgg
    15061 cggcccttac gacgcggcgc tggacgaaaa ccagctcagt gaggcctggg ccgacctggg
    15121 cgccggcctc ggtaccggcg caagcaccga ccgcatgagc ttttcgctgc gctcgctgag
    15181 ttacccggac ctgctgccca aggcggcgca gctggccgcc cggcagctgg gcgatccagc
    15241 cttcccggat gccgtctggc agcgcgagcg ccagcgcctg gccgcatcgc tgcgcgaggc
    15301 caagacacgg ccggccaccg tagcaggcca ggcctacgcc ccggcggtgt acggcgacca
    15361 cccgtacggc tacgagatga ccgaggaatc gctggagcgc atagcggtgc aggacatgat
    15421 gcaggtgtac cgccgcctga tccagccttg ccgtgccaag gtgagcatcg tgggcgcggt
    15481 cacacgggcc caggccgatg cgctggtgac gacgctgctc tcgcgcctgc cggcgggcca
    15541 ggcgggctgc gagccgctgc cgcccgtggc ggaggtgccg ccgctgacgg ctcccgtcgt
    15601 gcgcgagatt cccttcgctt cggcccaggc ccatgtcctg atcggccagc ccggctacaa
    15661 gcgcaacgac cccgatttct ttgccttggt gctgggcaat tacatcctgg gcggcggcgg
    15721 tttcgtgtcg cgcctgtcgc aggaagtgcg cgagaagcga ggcctgagct acagcgtcta
    15781 cagctatttt tcgcccggcc tgcatgcggg ggcgttcacc ctcggactgc agacgcggcc
    15841 cgaccaggcc gcgcaggcgg tgcaggtctc gcgcgaggtg ctggcgcggt tcgtcaccga
    15901 cgggccgacc gaagccgaac tcaaggccgc gaaggacaac ctgatcggcg gctttcccct
    15961 gctgatcga
//
LOCUS       DASWIY010000436         9069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_300470, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000436 DASWIY010000000
VERSION     DASWIY010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 9069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9069
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300470"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VGA59_15560"
     CDS             complement(<1..658)
                     /locus_tag="VGA59_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX9721135.1"
                     /translation="MTDEVEKKAALPEGWLEVDALDIEAQGVARRPDGKVVFIEGALP
                     FERVSVKVNRRKNNWEQGTLTQIHRESSQRVRPRCPHFGLHEGACGGCKMQHLHIGAQ
                     VAIKQRVLEDNLWHLAKVKAETVLRPIEGPTWGYRWRARFSVRHVRKKGTVLVGFHER
                     KSRYVADMRECHVVPPHVSDLLLPLRELIGSMDAIETCPQIELACGDEVTALVLRHLE
                     P"
     gene            complement(663..1463)
                     /locus_tag="VGA59_15565"
     CDS             complement(663..1463)
                     /locus_tag="VGA59_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9721136.1"
                     /translation="MNWPVLVFDIESIPDIAGLRSLRGSAPDSTDEQVYSGWCQERQD
                     KGQGDFMPLYLQRVLVISCVFRNAEGLRVHSFADRDNASEGKIIQTFFNTLEKHVPQL
                     VSWNGGGFDLPVLHYRGLRHGVVADKYWCMGEEGGADAREFKYNNYISRYHTRHLDLM
                     DLLAMYQPKNNAPLDAMARLCGFPGKLGMDGSQVYPAFLEGRIEDIRRYCETDVMNTY
                     LMYCRFQKMRCGLTEAEYEQEIAMVKATLGNLAPQEPHWQEYLAAWGE"
     gene            complement(1610..2518)
                     /gene="rpoS"
                     /locus_tag="VGA59_15570"
     CDS             complement(1610..2518)
                     /gene="rpoS"
                     /locus_tag="VGA59_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HEX9721137.1"
                     /translation="MDEVNPRDIPAEIAGESSDTLTLYLREVRRTELFTAEEEFEAAT
                     RARGGDFAARQSMIEHNLRLVVSIAKGYLGRGVPLSDLIEEGNLGLMHAIDKFEPERG
                     FRFSTYATWWIRQSVERAVMNQGRVIRLPVHVVRELQQVLRARRTLENDPAFAATRNG
                     FEGEGVRVEDVAALLGRDVQEVAELLAMAEAPKSLDAAMDRSDDEHTLGESMADELAI
                     DPTGVTHNHEVERLLNNWIDALSHREKEVLEGRFGLHDREPETLEVLSDRLGLTRERV
                     RQIQNEALLKLKRHMVRNGISKEALF"
     gene            complement(2632..3516)
                     /locus_tag="VGA59_15575"
     CDS             complement(2632..3516)
                     /locus_tag="VGA59_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX9721138.1"
                     /translation="MLELRSQGWIGAAAVLVVALLAGCAAKPREPAPVEDRGLGARVP
                     PPPVTAEPIKPLPGAENLGKPGYYTVRPGDTLIRIALDSGQNWRDIARWNNIDNPNMI
                     EVGQVVRVIPPPGVVPEVAVARPVPPVPAPPVAAASTPAASVPRTTATTPPQVASVPP
                     VVPTPAPAGEEDVPWGWPTTGAATVVASFDEQKNKGLDIAGKPGDPVLASADGRVVYA
                     GAGLRGYGNLIILKHNNTYLSAYAHNQTLLVKEDQTVRKGQKIAEMGSSDADRVKLHF
                     EIRRQGKPVDPARYLPAR"
     gene            complement(3530..4300)
                     /locus_tag="VGA59_15580"
     CDS             complement(3530..4300)
                     /locus_tag="VGA59_15580"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX9721139.1"
                     /translation="MSRRPAFPVRLDLSPAKPVDARVALAAGANAQGGAPGGMGLDSQ
                     AVRSRMVGKLVAQGVVDPRVLAAMQAVERHRFVDSALVNQAYEDTSLPIGLGQTISKP
                     SVVARMIELLLGGATDSSGRLGRVLEVGTGCGYQAAVLSVVAAEVYSIERLRGLHDKA
                     RANLRPFRLANVHLLFGDGMLGYAKGAPYAGILAAAGGEAVPQAWMDQLAIGARIVAP
                     TLMPGGQQALVMVEKTRRGMKQTVLEAVRFVPLKSGIA"
     gene            complement(4301..5077)
                     /gene="surE"
                     /locus_tag="VGA59_15585"
     CDS             complement(4301..5077)
                     /gene="surE"
                     /locus_tag="VGA59_15585"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX9721140.1"
                     /translation="MKILLSNDDGYQAPGIVALHEAIKDLGDVEVVAPEHNNSAKSNA
                     LTLHSPLYVHLAGNGFRYVNGTPADCVHVALTGLLGYRPDLVVSGINNGANMGDDTIY
                     SGTVGAAMEGYLFGVPSIAFSQTQKGWAHIDAAARKARELVQHVLANPHAAGHPWLLN
                     VNIPNLPYGELKPFKVCRLGRRHAAEKVISQTSPHGDLMYWIGGAGPAKDDAEGTDFH
                     ATAQGHVSITPLKVDLTDHDQLEHWARSAARLPAAMREGR"
     gene            5182..6156
                     /locus_tag="VGA59_15590"
     CDS             5182..6156
                     /locus_tag="VGA59_15590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX9721141.1"
                     /translation="MQAWLCENPVGVEALQWKELPTPQPGAGEVLIEIKAASLNFPDL
                     LIVQNKYQTKPPVPFVPGSEYAGVIAAVGEGVSHLKIGQSVACLSGTGGFATHTTAPA
                     ALCMPLPPGFPHVDAAAFIMIYATAYHALVDRAQLKAGETVLVLGAAGGVGTSAIQVA
                     KAMGARVIAAASSDEKCELCKSIGADATINYSRDNLRDAIKQLTDGKGPDVIYDPVGG
                     DLAEPALRSIAWRGRYLIIGFASGTIPALPLNLPLLKGASLVGVYWGSFAKHEPKANA
                     AMLAELARWYGDGKIKPVIDRTMPMAELPAALAHMGSRSVKGKLVMVN"
     gene            6186..6261
                     /locus_tag="VGA59_15595"
     tRNA            6186..6261
                     /locus_tag="VGA59_15595"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6219..6221,aa:His,seq:gtg)
     gene            complement(6350..8149)
                     /locus_tag="VGA59_15600"
     CDS             complement(6350..8149)
                     /locus_tag="VGA59_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9721142.1"
                     /translation="MSEPEDDNKTVLPRPLGASVTPSAGNPQAVETEEHNALPVGTRI
                     AEFEVKAIIGIGGFGIVYLAHDHSLGRDVALKEYLPTSLAYRSGLNVSVKTARQADTF
                     AAGLSSFINEGRVLAHFDNPSLVKVYRFWNANGTAYMVMPYYEGPTLKQALQDAAQAP
                     TEEWLKGLLLPLLSALELLHSENYFHRDIAPDNILLVKGKRPILLDFGAARRVIAGST
                     KALTVILKPGYAPVEQYFDSHEMTQGAWTDIYALAAVMHYAITGQAPVPSVSRLVKDS
                     ATPLAQAASGRYSDRFLRALDRALSVRPEQRPQTIGEFRELLGLRDAMADELPTASQD
                     RPKQKPGRKFIAVGGIAAGVIVVAAAAIMLATRDKAPSPSQDSAVVAEGPKDPPPPAS
                     NATVAPPIQAEQPVATPAAPPAPPIAAPPAADANRPFRPADALAQVYELRDPDHGVAV
                     NLEKTTVRVGKDKLRFRVQSDKPGYLYILMLGTDKQHIYLLFPNSLDQKNQTKANQPI
                     NLPRTGWTMTAGGPPGTNEFIALVSERPRDFKQIGLAKVGPFAELSLDNLAELIRSNT
                     GNTAVLAGTPICASGSACSPSYGAARFTIEEVN"
     gene            complement(8287..8967)
                     /gene="tagF"
                     /locus_tag="VGA59_15605"
     CDS             complement(8287..8967)
                     /gene="tagF"
                     /locus_tag="VGA59_15605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HEX9721143.1"
                     /translation="MSSSTDRAELARRFAWFGKLPSAGDFVSRRMPYALQQFWDRWCA
                     DGMDALKAASTVTGLEVWGGTPKWAFVLAAQPGVPTGQLGVFAPSCDRVGRVFPFVVT
                     APLVPDQQAGLLDRAASVGLAWGQVVAQAQQARLGIEAVDAGLHAALAETLATELEDD
                     DDDERTTLPLGLEPGPSPLPWPELNRNFDLHGSQSYWWSVPPPSTGFQTRMYTGPLKT
                     IHFLDLCR"
BASE COUNT         1508 a         3001 c         2897 g         1663 t
ORIGIN      
        1 gcggttccag atggcgcagt accagggccg tcacctcgtc gccgcatgcc agctcgatct
       61 gcggacaggt ctcgatcgca tccatggatc cgatcagctc gcgcagcggc agcagcaggt
      121 cgctcacatg cggcggcacc acatggcatt cgcgcatgtc ggcgacatag cggctcttgc
      181 gctcgtgaaa gcccaccagc accgtgccct tcttgcggac gtggcgcacc gaaaagcgcg
      241 cccgccagcg gtagccccag gtcggccctt cgatcgggcg cagcaccgtc tcggccttga
      301 ctttcgccag gtgccacagg ttgtcctcca gcacccgttg cttgatggcg acctgcgcgc
      361 caatgtgcag gtgctgcatc ttgcagccgc cgcatgcccc ctcgtgcagc ccgaagtgcg
      421 ggcagcgagg ccgcacgcgc tgggacgatt cccggtggat ctgcgtgagc gtgccttgct
      481 cccaattgtt cttcctgcgg ttgaccttga cggaaacccg ttcgaacggc agcgcgcctt
      541 cgatgaacac caccttgccg tcgggtcgcc gcgccacgcc ctgcgcctcg atgtcgagcg
      601 cgtcgacctc cagccagccc tcgggcaagg cggctttttt ctcgacttcg tctgtcatgc
      661 ccctactcgc cccatgccgc caggtattcc tgccaatgcg gctcctgcgg cgcgaggttg
      721 cccagcgtcg ccttgaccat ggcgatctcc tgctcgtact ccgcctccgt gaggccgcac
      781 cgcattttct ggaaacggca gtacatcagg taggtattca tcacgtcggt ttcgcaatag
      841 cgtcggatgt cctcgatcct gccttcgagg aaggccgggt acacctgcga gccatccatg
      901 ccgagcttgc cgggaaagcc gcacaacctc gccatggcat cgagcggcgc gttgttcttc
      961 ggctggtaca tggccagcag gtccatcagg tcgaggtggc gcgtgtggta gcggctgatg
     1021 tagttgttgt acttgaactc gcgcgcgtcg gcgccgccct cttcgcccat gcaccagtac
     1081 ttgtcggcga ccacaccgtg gcgcagcccg cgatagtgca gcaccggcag gtcgaagccg
     1141 ccgccgttcc agctgaccag ctggggaacg tgtttttcaa gcgtgttgaa aaacgtctgg
     1201 atgatcttgc cctcgctggc gttgtcccga tcggcgaacg aatgcacgcg cagcccctcg
     1261 gcattccgga aaacgcagct gatcaccagc acccgctgca gatacagcgg catgaaatcg
     1321 ccctggccct tgtcctggcg ttcctggcac catcccgaat agacctgctc gtcggtactg
     1381 tccggcgcgc tgccgcgcaa cgagcgcaag ccggcgatgt cgggaatgga ttcgatgtcg
     1441 aagacgagaa caggccagtt catggaagcg actgtaccag tcgcttccat gaactggcct
     1501 tcggccgtct cacgaaaccc cctccccggc cctccccctg acgggaggga ggaagaggct
     1561 tgtcattcga tcaaatcacg cgataaacgc gcgcgcgttt ttcacgtgct caaaaaagcg
     1621 cttccttgct gatgccgttg cgcaccatgt ggcgcttgag tttgagcagt gcctcgttct
     1681 ggatctgacg cacgcgctcg cgcgtgaggc ccagccggtc gctaaggacc tcgagcgtct
     1741 cgggctcgcg gtcatgcagg ccgaagcggc cctccagcac ttccttctcg cgatgggaca
     1801 gcgcgtcgat ccagttgttc aggagccgct cgacctcgtg gttgtgcgtc acgccggtag
     1861 ggtcgatcgc caattcgtcc gccatcgact cgcccagggt gtgctcatcg tccgagcgat
     1921 ccatcgccgc gtcgagcgac ttcggtgcct cggccatggc gagcaattcg gccacctcct
     1981 ggacgtcgcg gcccagcagg gcggccacat cctcgacgcg cacgccctcg ccttcgaagc
     2041 cgttgcgcgt ggccgcgaac gcgggatcgt tctccagcgt ccggcgcgca cgcagcacct
     2101 gctgcagctc gcgcaccaca tgcaccggca accggatcac ccggccctgg ttcatcaccg
     2161 cccgctcgac cgactgccgg atccaccagg tcgcgtaagt ggaaaagcgg aaaccgcgct
     2221 cgggctcgaa cttgtcgatg gcatgcatca ggccgagatt gccttcctcg atcaggtccg
     2281 acagcggcac gccgcggccc aggtagccct tggcgatgct cacgaccagg cgcaagttgt
     2341 gctcgatcat ggactgccgc gcggcgaagt cacccccgcg tgcgcgggtg gcagcctcga
     2401 attcctcctc ggcggtgaaa agctcggtgc gcctgacctc gcgcaggtac agcgtcaatg
     2461 tgtcgctgga ctccccggcg atctccgccg gaatgtcgcg cgggttcacc tcatccatcg
     2521 gctcgctgcc ggcaaggctg tccaccagcg gtatcggagc gggcacagcg ccgccgtcca
     2581 ggctcgcagc cggaaccgcg ggcgggccgt atccgttgcg ctttttcatt cttacctcgc
     2641 gggcaagtac ctggccgggt ccacgggctt gccctgccgc cggatttcga aatgcagctt
     2701 gacacggtcc gcgtcgctgc tgcccatctc cgcgatcttc tgccccttgc gcacggtctg
     2761 gtcttccttg acgagcaggg tctggttatg ggcgtatgcc gaaaggtaag tgttgttgtg
     2821 cttcaggatg atcaggttgc cgtagccgcg cagcccggcg cccgcataga ccacgcgccc
     2881 gtcggccgag gccagcaccg gatcgccagg tttgcccgca atgtccaggc ctttgttctt
     2941 ttgttcgtcg aagctcgcca caaccgtcgc ggccccggtc gtgggccagc cccagggcac
     3001 gtcttcctca ccggcgggcg cgggcgtcgg gaccaccggc ggcacgctgg ccacttgcgg
     3061 gggcgtggtc gccgtggtgc gcggcacgct tgccgcgggt gtagacgccg ccgcgacggg
     3121 tggcgccggg accggtggca ccggccgtgc caccgcgact tcgggcacta cgcctggcgg
     3181 cgggatcaca cgcacaacct gcccgacctc gatcatgttg ggattgtcga tgttgttcca
     3241 gcgtgcgatg tcgcgccagt tctgtccact gtccagcgca atgcggatca aggtatcgcc
     3301 ggggcgcacg gtgtagtagc cgggcttgcc gaggttctcg gcaccgggca gtggcttgat
     3361 gggttccgcc gtcaccggcg gcggcggcac acgcgcgccg aggccgcgat cctccacagg
     3421 ggcgggctca cgcggcttgg ccgcacagcc cgcgagcaag gcgaccacca gcaccgccgc
     3481 cgcccctatc catccctgac tccgcaactc gagcatcttt tattttcctt caagcgatgc
     3541 ccgattttag ggggacaaag cgaacagcct caagcacagt ttgtttcatt ccgcgccgcg
     3601 tcttctcgac catcaccaac gcttgctggc caccgggcat gagcgtcggc gcaacgatgc
     3661 gcgcccctat cgccaactga tccatccagg cttgcggcac ggcctcgccg cccgccgccg
     3721 ctaggatccc ggcataaggt gcacctttgg cgtagccgag catgccgtcg ccgaacagaa
     3781 gatgcacgtt ggccaggcgg aacggccgca gattggcgcg cgccttgtcg tgcaggccgc
     3841 gcaggcgctc gatgctgtaa acctcggccg ccaccacgct cagcacggcc gcctggtagc
     3901 cgcagccggt gccgacctcc agcacccgcc ccagcctgcc cgaggaatcc gttgctccac
     3961 cgagcagcaa ttcgatcatg cgcgccacga cactgggctt ggagatggtc tggccaaggc
     4021 ctatgggcag gctggtgtcc tcgtaagcct ggttcaccag cgcgctgtcg acaaagcggt
     4081 ggcgctccac tgcctgcatg gccgccagca cccgcggatc gacgacaccc tgcgcaacca
     4141 gtttcccgac catgcgcgag cgcaccgcct gtgaatcgag acccatgccc cccggggcgc
     4201 cgccttgcgc gttcgcgccg gcggcaagcg ccacccgcgc gtcgacgggc ttcgcggggg
     4261 acaagtccag gcgcacggga aaggcgggac gccgcgacat tcagcggcct tccctcatcg
     4321 ccgccggcag ccgggccgcg gaccgcgccc aatgctccag ctgatcgtgg tcggtcaagt
     4381 ccaccttcaa cggtgtgatc gacacgtggc cctgcgccgt ggcgtgaaag tccgttcctt
     4441 cggcatcgtc cttggccgga cccgcgccgc cgatccagta catgaggtcg ccatgcgggc
     4501 tggtctggga aatcaccttc tcggcggcat ggcgccgccc gaggcggcac accttgaacg
     4561 gcttgagttc gccgtagggc agattcggta tgttgacgtt gagcagccag ggatggccgg
     4621 cggcgtgggg gttcgcgagc acgtgctgca ccagttcgcg cgccttgcgt gccgcggcat
     4681 cgatgtgcgc ccagcccttt tgcgtctgcg agaaagcgat cgacggcacg ccgaacaggt
     4741 agccttccat cgccgcgccc acggtgccgg agtagatcgt gtcgtcgccc atgttggcgc
     4801 cgttgttgat gccgctgacg accaggtcgg gccggtagcc cagcagcccg gtcaatgcca
     4861 cgtgcacgca atcggccggt gtcccgttga catagcgaaa gccatttccg gccaggtgca
     4921 cgtacagggg ggaatgcaag gtgagcgcat tggacttggc gctgttgttg tgttcgggtg
     4981 cgacgacctc gacgtcgccg aggtccttga tcgcttcatg gagcgcgacg atgccgggtg
     5041 cctggtagcc gtcgtcgttg gaaagcagaa ttttcatcgc cgtaaaggat tgaaaagatt
     5101 gtaggtgcga aggggtcgcc gcagagacat tccgcggcaa tcattgcccc tatcatcgcc
     5161 agttttcatc cggaggatgc gatgcaagcc tggctttgcg agaaccctgt cggcgtcgag
     5221 gcgctgcaat ggaaggaatt gccaacgccg cagcccgggg ccggcgaagt gctgatcgag
     5281 atcaaggcgg cgagcctgaa ctttcccgat ctgctcatcg tgcagaacaa gtaccagacc
     5341 aagccgccgg tgccctttgt gccgggctcg gagtacgccg gcgtgatcgc ggccgtcggc
     5401 gagggggtca gccatctcaa gatcggccag agtgtggcct gcctgtccgg caccggcggc
     5461 tttgccactc acaccacggc ccccgcggcc ctgtgcatgc cgctgccgcc gggctttccc
     5521 cacgtggacg cggccgcgtt catcatgatc tatgcgaccg cgtaccacgc actggtggac
     5581 cgcgcccagc tgaaggccgg cgagacggtg ctggtgctgg gcgccgccgg cggcgtcggt
     5641 acttcggcca tccaggtcgc gaaggccatg ggtgcgcgcg tcatcgcggc ggcctcgtcc
     5701 gacgaaaagt gcgagctgtg caagtcgatc ggcgcggacg cgacgatcaa ctacagccgg
     5761 gacaacctgc gcgacgcgat caagcaattg acggacggca aaggcccgga tgtgatctat
     5821 gaccccgttg gcggcgacct ggccgaaccg gcgttgcgtt ccattgcctg gcgcggccgc
     5881 tacctgatca tcggcttcgc ctcgggcacg atccctgcgt tgccgctgaa cctgccgctg
     5941 ctcaagggcg catcgctggt cggcgtgtac tggggcagct ttgccaaaca cgaacccaag
     6001 gccaacgccg cgatgctggc ggagctggcg cgctggtacg gcgacggcaa gatcaagccg
     6061 gtgatcgacc gcacgatgcc gatggccgag ctcccggccg cccttgccca catgggctca
     6121 cgcagcgtga agggcaagct ggtgatggtg aattagccgg cccggtataa ttcgcgttct
     6181 ctgcggtggc tgtagctcag ttggtagagt ccaggattgt gattcctgtc gtcgtgggtt
     6241 cgagtcccat cagccacccc atccaggcca agctattgat ccgcttggcc tttgtcttta
     6301 gagccggcat agcttcccag ccgcatcccc aagccgctga acctgcgctt caattgacct
     6361 cttcgatcgt aaagcgggcg gcaccgtaag aaggggagca ggcgctgcca gacgcacaga
     6421 tcggcgtccc ggcaagcacg gcggtgttgc cggtgttcga tcggatcagt tccgccagat
     6481 tatccagtga gagctcggca aagggcccca ccttggccaa tccgatctgc ttgaagtccc
     6541 gtggccgctc ggaaaccagc gcgatgaact cgttcgttcc cggcgggccg cccgcggtca
     6601 tggtccaacc agttcttggc aggttgatgg gctgattggc cttcgtctgg ttcttctggt
     6661 cgagcgaatt cggaaagagc aggtagatgt gctgcttgtc cgttcccagc atcaggatgt
     6721 acaagtaccc cggcttgtcg gactgcaccc ggaaccggag cttgtccttg cccacgcgca
     6781 cggtcgtctt ttccaggttg acagcgacgc cgtgatcggg atcgcgcagt tcatacacct
     6841 gtgccagggc gtccgcgggt ctgaagggcc ggtttgcatc ggcggccggc ggcgcggcta
     6901 tcggcggggc cggtggcgcg gccggcgttg cgacaggctg ttcggcctgg atcggcgggg
     6961 ctacggtggc attggacgca ggcggcggtg ggtctttcgg cccttcggcc acgacggctg
     7021 aatcttggct gggggatggc gctttatccc gcgttgcgag catgatggcc gccgccgcaa
     7081 ccacgattac accggcggca atcccgccga cggcgatgaa cttgcgaccc ggcttttgtt
     7141 tgggccgatc ctggctcgcg gtcggcagct cgtctgccat tgcgtcacgc aggccaagca
     7201 gttcgcggaa ctccccgatg gtctgagggc gctgttccgg gcgtaccgac aaggcccgat
     7261 ccagtgcgcg tagaaagcga tcgctatatc ggccggaggc cgcctgcgca agcggagtcg
     7321 ccgagtcctt gaccaggcgg ctgacggaag gtacgggagc ttgccccgtg atcgcatagt
     7381 gcatcacggc agccagcgcg taaatatccg tccacgcgcc ttgcgtcatt tcatggctgt
     7441 cgaagtactg ttcgaccggc gcatatcccg gcttcaggat gaccgtcagc gccttggtac
     7501 taccggcaat gacgcgccgc gccgcgccga aatcaagcaa tatcgggcgc tttcccttga
     7561 ccaggagaat gttgtcggga gcgatgtccc ggtggaagta attctcgctg tgaagcagtt
     7621 ccaatgcgga cagcagaggc aggagcaagc ccttcaacca ctcctcggtc ggcgcttgcg
     7681 ctgcatcctg aagggcctgc ttcagcgtcg gcccctcgta atagggcatg accatgtatg
     7741 cggttccgtt ggcattccag aaccggtaga ccttgacgag cgatggattg tcgaagtgcg
     7801 cgaggacccg gccttcgttg atgaagctcg aaaggcccgc ggcaaacgta tcggcctgtc
     7861 gagccgtttt cacgctgaca ttaagcccgc tcctgtaggc aagcgaggtc ggaaggtact
     7921 ccttgagcgc cacgtcccgg cccagggaat ggtcgtgggc gagatagaca atgccgaatc
     7981 caccgattcc gatgatggct ttgacctcga actcggctat gcgggtgccg acaggcaacg
     8041 cattgtgttc ctcggtttca acggcttgcg ggtttcccgc ggatggagtg accgacgcac
     8101 caagcggcct gggcagcacg gtcttgttgt catcttcggg ttcggacata gcgtttgccg
     8161 tcaagaagct gaagtgaagc cgccaaagat gaggaaaagg ctgatccgcg gttcaccttc
     8221 agctcgcatt atcgacctgc ggggtcttca ctgcattggc ggatcttcct aggtccactc
     8281 agtgaactaa cggcacagat ccaggaaatg aatagtcttg agcgggcccg tgtacatgcg
     8341 agtctgaaag ccggtgctcg gggggggcac gctccaccaa taactctgtg agccgtgcag
     8401 atcgaaattg cggttcagct cgggccatgg cagaggcgaa gggcctggct caagtcccaa
     8461 cggtaaggtg gttctttcat cgtcgtcgtc gtcttccagc tcggtggcca gcgtttcggc
     8521 cagcgccgca tgcaaaccgg cgtcgaccgc ctcgatcccc agccgggcct gctgtgcctg
     8581 ggcgacgact tggccccaag ccagtcccac cgaggctgcg cggtccagca acccggcctg
     8641 ctgatcgggc accagcggcg cggttacgac gaacgggaag acgcggccca cgcggtcgca
     8701 cgagggcgcg aaaacgccca gctgcccggt ggggacgccg ggctgcgcgg ctaacacgaa
     8761 cgcccacttg ggtgttccac cccagacctc caggccggtt acggtactgg cggctttcag
     8821 cgcgtccatg ccatcggcgc accagcggtc ccaaaactgc tggagggcat aaggcatgcg
     8881 ccgggacacg aagtcgcccg cgctgggcag cttgccaaac caggcgaagc ggcgcgcaag
     8941 ctccgcgcgg tccgtcgatg acgacatgtc aacctcgccc cgggcaggca aaaccctcca
     9001 gctgcggcaa tcgcagaggg ttgcgcagcg actgcgcggt gaactcgatg cccaccgtgc
     9061 gtccatcca
//
LOCUS       DASWIY010000437         4523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2531195, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000437 DASWIY010000000
VERSION     DASWIY010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531195"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VGA59_15610"
     CDS             <1..303
                     /locus_tag="VGA59_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX9721144.1"
                     /translation="LVSQHRVRKRLKRLRYLAEFVAPLFAARKGRDFIAALKPVQDAL
                     GLYNDELMALEAYRRLAEREPAAWFGVGWLTARRAGNAAKCQRELERFAKAKPFWR"
     gene            complement(359..1843)
                     /locus_tag="VGA59_15615"
     CDS             complement(359..1843)
                     /locus_tag="VGA59_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX9721145.1"
                     /translation="MQNGTLLAAVDLGSNSYRLEIGRFDHGQIHRTEYIKETVRQGHG
                     LDEARKLTPEAMQRGWDCLARFGERLAGFRRSQVRAVATQTLREARNRDEFLRRAQQV
                     LGFPIDVISGREEARLIYLGVAHLLPQSDERRLVVDIGGGSTELILGCQFDAAVTESY
                     RVGSANWSAKYFPQGGFTAAAFESAEVAAKAVLDGALSTYRRESWDIAYGSSGTIGAV
                     SDILGAAGWEGGTIDRDGLDWLQERLLAARSAEKVRLEGLKDDRRPVIGGGVSVLRAV
                     FDLLEIDEMQPALGALRHGALFDLLDREHGETDIRSTTVQRLAARFGADPAQAQRVAK
                     VACRLLRQLRAQDNNDDLARHARKLGWAAQLHEIGSAVSHSDYHKHGAYILDNADALG
                     FALPELHRLGLLVLGHRGKLRKLDADFEDELFVHQLFCLRLAVILCHARRDPDLKGLQ
                     ARAAGHEVTISLRPGWGEAFPQSAHLLREEAVAWQKTPWRLVLA"
     gene            complement(1950..3092)
                     /locus_tag="VGA59_15620"
     CDS             complement(1950..3092)
                     /locus_tag="VGA59_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9721146.1"
                     /translation="MTTIFPTQWRRRTLLAAAASALADPAWTQPGRSAAAGRPITAAQ
                     IFDSSAGQQDVSKDFLIGSRAAWQDINAKGGVRGRPVVHSSIEVNGAPASLRAAMAAV
                     RDNPACVALSGTSGDPVATEVARLLLQENVAITHAAPWLQNSSVEVDDRTFPIFAARQ
                     EQIAHVLRSLSVIGVQEVGAVYASEQEFRLYQEDLRRTAAEFKLRLQVFRSEGDLVRL
                     GQRLTAQTPSILLFLGGTPELVQFTQGLEKQSRQRYVAALADVNLQTLMQMGAARNTP
                     VIATQAVPMVNASLPIVRAYRDTLARLFDEPPTPLSLAGFIAARYTFEVLSDVDGALT
                     RQSALAAFQRRSSLDVGGYRVAFDPRRRSGTFVTQSMLTTDGRVVG"
     gene            3355..4389
                     /gene="pstS"
                     /locus_tag="VGA59_15625"
     CDS             3355..4389
                     /gene="pstS"
                     /locus_tag="VGA59_15625"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX9721147.1"
                     /translation="MKISTRIATLGLAGLSFAGLAAAQDITGAGATFPAPLYAKWAAD
                     YNKATGIKINYQSVGSGAGIKQIDSKTVAFGASDMPLKDEELAKKGQMQFPTVIGGVV
                     PVINIKGIAPGQLKLNGQVLGDIYLGKITKWTDPAIKALNPSLPLPDAAISPVRRADG
                     SGTTFIFTNYLSKVNPEWKAKVGEGTAVNWPAGAGGKGNEGVAAFVNRLPNSIGYVEY
                     AYVKQSKMTYAQMQNTAGNFVSPDDTAFKAAAAGADWAKSFYQILTNQPGKDAWPITG
                     ATFILMHKTQDKPSEAAAALKFFEWAYKNGDKTADGLDYVPMPAVVKTQIEKAWAGIK
                     DASGKTIAYK"
BASE COUNT          804 a         1524 c         1472 g          723 t
ORIGIN      
        1 ctggtgtcgc agcaccgcgt gcgcaagcgg ctcaagcgcc tgcgctacct cgcggagttc
       61 gtggcgccgc tctttgccgc gcgcaagggc cgggatttca tcgccgcact gaagccggtt
      121 caggacgcac tgggccttta caacgacgag ttgatggcgc tcgaggccta tcggcggctc
      181 gccgaacgcg agcccgcggc ctggttcggc gtgggctggc tcacagcccg gcgtgcgggc
      241 aacgccgcga aatgccagcg ggagctcgaa cgcttcgcca aggccaagcc tttctggcgt
      301 tgaaacggcg cgcggcgcgc tcaggaccgc accgccgtat gcccagccag ccgaagccct
      361 acgccaggac cagcctccaa ggagtcttct gccacgctac ggcctcttcg cgcagcaagt
      421 gggccgactg cgggaacgcc tcgccccacc cggggcgaag gctgatcgtc acctcgtggc
      481 ccgctgcacg ggcctgcagg cccttcaggt cagggtcgcg ccgcgcgtgg cacaggatca
      541 ccgccaggcg aaggcagaac agctggtgta cgaacaactc gtcctcgaaa tccgcatcca
      601 gcttccgcag cttgccccgg tgccccagca ccagcaggcc gagccggtgc agctccggca
      661 acgcgaagcc cagggcgtcc gcattgtcga ggatgtaggc gccgtgcttg tggtaatcgc
      721 tgtgcgagac ggcgctgccg atctcgtgca attgggcggc ccatcccagc ttgcgcgcgt
      781 gacgcgcaag atcgtcgttg ttgtcctggg cgcgcaactg gcgcagcaac cggcatgcca
      841 ccttggccac gcgctgggcc tgtgccggat cggccccgaa cctggcggcc aggcgctgca
      901 cggtggtgga acggatgtcc gtttcgccgt gctcgcgatc cagcaggtcg aacagcgcgc
      961 cgtggcgaag tgcgcccagc gccggctgca tctcatcgat ctcgagcagg tcgaacacgg
     1021 cgcgcaggac gctcacgccg cctccgatga cgggacggcg atcgtctttc aagccttcca
     1081 gccgcacctt ctcggcgctg cgcgccgcca gcagcctttc ctgcagccag tcgaggccgt
     1141 cgcggtcgat ggtcccgcct tcccatcctg ccgcgcccag aatatcgctc acggcgccga
     1201 tggtgccgga ggagccgtag gcgatgtccc agctttcgcg gcgatacgtg ctgagtgcgc
     1261 cgtcgagcac cgctttggcg gcaacctcgg ccgattcgaa ggcggcagcg gtgaaaccgc
     1321 cttgcggaaa atacttcgcc gaccagttgg cgctgcctac gcgatagctc tccgtgaccg
     1381 cggcatcgaa ctggcagccc aggatgagct cggtggaccc gccgccgatg tcgaccacca
     1441 gccggcgctc gtcggactgg ggcagcaggt gtgccacccc aaggtaaatc agccgcgcct
     1501 cttcgcggcc cgatatcacg tcgatcggaa agccgagcac ctgctgagcc cggcgcagga
     1561 actcatcgcg gttgcgtgct tcgcgcaacg tctgggtcgc gaccgcacgc acctgagatc
     1621 gccggaaacc ggccagccgc tcgccgaagc gcgcgaggca atcccagccg cgctgcatgg
     1681 cttccggggt gagcttgcgc gcctcgtcca ggccatggcc ctggcgcacg gtttccttga
     1741 tgtattcggt gcggtggatc tggccgtgat cgaagcgccc gatctccagg cgataactgt
     1801 tggagccgag gtcgacggcg gcgagaaggg taccgttttg catgccaaag gggtgtggag
     1861 tgctgcgggg cagcttagca cccgcagcgc gtccgcagag ccgctgcgga tgaatgcgcg
     1921 ggcgcgcagc cgggagcaac tgcacagccc taaccgacga cccggccgtc cgttgtcaac
     1981 atgctctgcg tgacgaaggt gccgctgcgg cggcgcggat cgaaggcgac gcggtagccg
     2041 cccacgtcca ggctgctgcg ccgctggaag gcggccaatg cgctttggcg cgtcaaggcg
     2101 ccatcgacgt cgctgagcac ctcgaacgtg tagcgtgccg cgatgaaacc cgccaggctc
     2161 agcggcgtcg gcggctcatc gaaaaggcgt gccagcgtat cgcggtaggc gcgcacgatg
     2221 ggcaggctgg cgttgaccat gggtaccgcc tgggtagcga tcaccggggt gttccgtgca
     2281 gcacccatct gcatcagggt ctgcaggttc acgtccgcca gggcggccac gtagcgctgg
     2341 cgagactgct tttccagccc ttgtgtgaac tggaccagct cgggcgtgcc gcccaggaac
     2401 agcaggatgg agggcgtctg cgcggtcagg cgctggccca gacgaaccag atcgccctcg
     2461 gagcggaaaa cctgaagcct gagcttgaat tcggccgccg tgcgcctcaa atcttcctgg
     2521 tacaggcgga attcctgttc tgacgcatag actgcgccga cctcctgtac gcctatcacg
     2581 ctgagtgacc gcagcacgtg cgcgatctgc tcctgccggg ccgcgaagat gggaaaagtg
     2641 cgatcgtcca cttcgacgct ggaattctgc agccacggtg cggcatgcgt gatcgctacg
     2701 ttttcctgaa gcagcagtcg cgccacctcg gtggcgacag ggtcgccgga ggttcccgac
     2761 aacgcgacac aagccgggtt gtcgcgcacg gcggccatcg ccgcgcgcag actggccggc
     2821 gcgccattga cctcaatcga gctgtgcact actggccggc ctcgcacgcc gcccttggcg
     2881 ttgatgtcct gccacgcggc gcgcgagccg atcaggaagt ccttggaaac gtcctgctgc
     2941 cctgcggaac tgtcgaagat ctgggccgcc gtgatgggac ggccagccgc agccgaacga
     3001 ccgggctggg tccaggcagg atccgccaag gctgacgctg ccgcggccag cagcgtgcga
     3061 cggcgccact gggtcggaaa tattgttgtc atgactgccc catgtgccga tcaatcgtgc
     3121 actggcgcca tcagcacgcg gtcgacgccg ttcggcatgg gttttcctcc ggtcgaagta
     3181 gtgctttgca gcctatggcc ggaccatgac agtttcgtga ccacaccatg acttgccgat
     3241 gacacgcagc caccggcgac accggggatg gaggcccctt gtcgcctgtc acgccactgt
     3301 cacagaagct tcttagagtc tgcccatccc gattcccaac ccagaggacc aacgatgaaa
     3361 atcagcacgc gaattgccac cctcggcctg gctggccttt cctttgcagg cttggcggcc
     3421 gcgcaggaca tcaccggcgc cggcgccaca ttcccggcgc cgctgtacgc caagtgggcg
     3481 gccgattaca acaaggccac tggcatcaag ataaactacc agtcggtcgg ctcaggcgcc
     3541 ggtatcaagc agatcgactc caagacggtg gccttcggcg cgtccgacat gccgctgaag
     3601 gatgaggaac tggccaagaa gggccagatg cagttcccca cggtgatcgg cggcgtggtg
     3661 ccggtgatca acatcaaggg catcgcgccc ggccaactca agctcaacgg ccaggtcctg
     3721 ggtgacatct acctgggcaa gatcaccaag tggaccgacc ctgccatcaa ggcgctgaac
     3781 ccgtcgctcc cgctgccgga cgcggcgatt tcgccggtgc gccgcgccga cgggtcgggc
     3841 accaccttca tcttcacgaa ttacctgtcc aaggtgaacc ccgagtggaa agccaaggta
     3901 ggcgagggca cggcggtgaa ctggccggcc ggcgcgggtg gcaagggcaa cgaaggcgtt
     3961 gccgctttcg tcaatcgcct gcccaattcc atcggctacg tcgagtacgc ctacgtcaag
     4021 cagagcaaga tgacctatgc ccagatgcag aacacggcgg gtaatttcgt ctcgcccgac
     4081 gacacggctt tcaaggccgc ggcggccggc gccgactggg caaagagttt ctaccagatc
     4141 ctcaccaacc agcccggcaa ggatgcctgg ccgatcaccg gcgccacttt catcctgatg
     4201 cacaagaccc aggacaagcc cagcgaagca gccgccgcgc tcaagttctt cgaatgggct
     4261 tacaagaacg gcgacaaaac ggccgatggc ctggactacg tgccgatgcc ggcggtcgtg
     4321 aagacccaga tcgaaaaggc ctgggccggt atcaaggacg cgtcgggcaa gaccatcgca
     4381 tacaagtgac gtcggcgggg acagcgcaat gttggcaccg ggccggctga tgtcctctac
     4441 acttccggcc catgagaccg cattcgacct ggccggcatg ggccatgcac gcgagatgac
     4501 gccccctccg gggcgccccg tgc
//
LOCUS       DASWIY010000438         5071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1415232, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000438 DASWIY010000000
VERSION     DASWIY010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415232"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VGA59_15630"
     CDS             <1..182
                     /locus_tag="VGA59_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721148.1"
                     /translation="CGVVESVALPNQQGAFQVRIRMDDGSLRTVEQSGPVAAGARVLL
                     EGTSVRVLQAGPRQG"
     gene            complement(437..1474)
                     /locus_tag="VGA59_15635"
     CDS             complement(437..1474)
                     /locus_tag="VGA59_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX9721149.1"
                     /translation="MSSITTFANRQVRLAKRPLGIPTRDDWSFTTEPVQPPAAGGVLI
                     KTLALSLDPAMRGWMNDAKSYIPPVGIGEVMRAGGIGKVIASSNSQYAVGDLVSAALG
                     AQEYLTVGQDEIKRIGMFKIDPRLGNITQWLNVLGMPGMTAYFGLLDVGQPRPGDTVV
                     VSGAAGAVGQTVGQLARIKGCRVVGIAGGRAKCDWVVDELGFDACIDYKAGNVKAGLK
                     EHCPKGVDIYFDNVGGEILDDVLARLARKARIVICGAISQYNNTTPVQGPKNYLSLLV
                     NRARMEGMVVFDYADRFPVAVAELAGYLKDGRMKSREDVVLGLDTFPEALVKLFTGEN
                     FGKLVLQVADA"
     gene            2083..3345
                     /gene="egtB"
                     /locus_tag="VGA59_15640"
     CDS             2083..3345
                     /gene="egtB"
                     /locus_tag="VGA59_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX9721150.1"
                     /translation="MPATPWSVRADTPAEPLRERVAARYAGIRAHSVALAAPLSAEDQ
                     CVQSMPDASPTKWHLAHTSWFFEAVVLAPHVPGYEPFDARFFYLFNSYYESLGPRHPR
                     PQRGLLTRPGLAEVHAYREHVDAAMRRFVGEAGAKTWEVAGPLVELGLNHEQQHQELI
                     LTDILQALSCNPLLPAYQGGEAPALRLAAATQPLNWIGMPGGTVKVGHAGEGFAFDNE
                     TPRHDVLLAPYRIADCLVTCGEYAQFIADGGYERVQLWLSDGWAAVQAGGWKAPVYWI
                     APGDARAPAAHWQVFGLEGVRPLDPAAPVSALSFYEAAAYAEWAGARLPTEFEWEAAC
                     DAPGITHMLGQAWQWTRSSYDPYPGFRPLAGAVGEYNGKFMVGQLVLRGSSRATPQGH
                     ARPTYRNFFPPAARWQFTGLRLAKDAAC"
     gene            3402..4331
                     /gene="egtD"
                     /locus_tag="VGA59_15645"
     CDS             3402..4331
                     /gene="egtD"
                     /locus_tag="VGA59_15645"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX9721151.1"
                     /translation="MPAGAFAHDMMRALASRPRSISPKYFYDAKGSRLFDRICELPEY
                     YPTRTELRILADNAHEIAVQMGPRAEIVEFGAGSLRKVRLLLDAMDRPARYLPIDISG
                     EHLAHSATALQRDYPGLDVQPVVADYTQRLLLPARVPGAGQRVGFFPGSTIGNFTPQE
                     ALHFLQVAGQVLRGGALLLGADLVKDPAVLHAAYNDAEGVTAAFNLNLLERANRELGT
                     RFALDQFAHSAFYNAPQQRIEMHLVSRRRQKVELGGDCYELEEGETLHTESSHKFTVE
                     GMRALAIGAGFGPGPVWTDADRLFSVHWLQAPS"
     gene            4355..4636
                     /locus_tag="VGA59_15650"
     CDS             4355..4636
                     /locus_tag="VGA59_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX9721152.1"
                     /translation="MKATWNGTLIAQSDDTVVMDGNHYFPAASLNREYVTFSNHHTSN
                     PLKGEASYYSLLVNGELKADAVWYYPDPKPEVEMLKDRVAFGNGIKIEP"
BASE COUNT          807 a         1665 c         1719 g          880 t
ORIGIN      
        1 actgcggcgt ggtggaatcg gtggcactgc cgaaccagca aggggccttc caagtgcgca
       61 tccgcatgga cgacggctcg ctgcgcacgg tcgagcagag cgggccggtg gccgccgggg
      121 cgcgggtgct gctcgagggc acatccgtcc gcgtcttgca ggcgggcccg cggcagggct
      181 gaactgaata cgggaactct gcgcgcctcc cggcctgcgc cgggatggtc ctgcgcgcga
      241 acgcttacgc gtccgcaatc tgcaagcatt gtcattgcgg gcttgaaccg caatccatga
      301 ccctgaattc atggatcccg gatcaagtcc gggatgacaa gtccatggtc ctgcgcgcga
      361 acgcttacgc gtccgcaacc tggcaacctg acgcgtggat cccggcctgc gccgggatgg
      421 tcctgcgcgc gaacgcttac gcgtccgcaa cctgcaggac caacttcccg aagttttcgc
      481 cggtgaacaa cttcaccagc gcctccggaa atgtgtccag ccccaagacc acgtcttccc
      541 tgctcttcat gcggccgtcc ttcaggtagc cggccaactc ggccacggcg accgggaagc
      601 gatcggcata atcgaacacg accatgcctt ccattcgcgc ccggttgacc agcaacgaca
      661 ggtagttctt cggcccctgc acgggcgtgg tgttgttgta ctggctgatc gccccgcaga
      721 tgacgatgcg cgccttgcgt gccagccgcg ccagcacgtc gtccaggatt tcgccgccca
      781 cgttgtcgaa gtagatgtcc acgcccttgg ggcagtgttc cttcagcccg gccttgacgt
      841 tgcccgcctt gtagtcgatg catgcatcga agcccagctc atcgaccacc cagtcgcatt
      901 tggcgcgccc gccggcgatg cccacgacgc ggcagccctt gatcctggcc aactggccga
      961 cggtctgtcc cactgcgccg gccgcgcccg acaccacgac ggtgtccccg ggccgcggtt
     1021 ggccgacgtc cagcaggccg aagtacgcgg tcatgccggg catgcccagc acattgagcc
     1081 actgggtgat gtttcccagc cgcggatcga tcttgaacat gccgatgcgt ttgatctcgt
     1141 cctgcccgac cgtgaggtat tcctgcgcgc ccagggcggc ggacaccaaa tcgccgacgg
     1201 cgtactggga attgctcgag gcgatgacct tgccgatgcc gccggcgcgc atgacctcgc
     1261 cgatgccgac cggcggaatg taactcttgg catcgttcat ccagccgcgc atggccgggt
     1321 ccagcgacag ggccagggtc ttgatcagca cgccgccggc ggccggtggc tgcaccggct
     1381 cggtcgtgaa ggaccagtcg tcgcgtgtcg gtatcccgag cgggcgcttg gccaggcgga
     1441 cctggcggtt ggcgaaggtg gttatcgaac tcatttagtc tcccggcgtg gcgttctgca
     1501 aggacaggga tggtagcggc tggtgtccgg gcggccggta gcgcaagtga cagcaatccc
     1561 gggagccgcg tctatccgca agcgatgatc gcttcgcgca gcacgctcaa atggctggtg
     1621 cgtcgcatcg gcggggtact cggccacaag actgtgtgag ctttcttgtc agttctccat
     1681 ggcgctgcgc gccacccgca gcgtatgaat tcgcgcggtc agtgcggggg ccaccgcgga
     1741 accggctttg ccgggccgct ggtgggcctt gcaggccgca tggccgcgag acgcggccat
     1801 tcctcgccct gtccaaaccg acgcaggtcg gtttggagcc gcaggtctcg gccccctcga
     1861 gggggagagg gcggagcccg agggggcacc gccgaaggcg cttcgggggt gggctttcac
     1921 ttcaggaggg ggctgcctgc gcccgtcccg cggcgcgcgg ccggtgcaca gtttcgcgct
     1981 cttatgctgt aatgaatcgg gagggacttc gacagatgtg tttgggcccg tgccggacca
     2041 gggcggcgcg gggatcgatg cgaaaccatt ggaggccggc ctatgcccgc taccccctgg
     2101 tccgtgcgag cggacacccc tgccgagccg ttgcgcgagc gcgtggcggc gcgctacgcc
     2161 ggcatccgag ctcattccgt ggcgctggcc gctcccttgt cggcggaaga ccagtgcgtc
     2221 cagtcgatgc ccgacgccag cccgaccaaa tggcacctgg cgcataccag ctggtttttc
     2281 gaggccgtgg tgctggcgcc gcacgtcccg ggttacgagc ccttcgatgc acgctttttt
     2341 tatcttttca attcttatta cgagtcgctt ggcccgcgcc atccgcggcc ccagcgcggc
     2401 ctgttgacac ggcccggcct ggccgaggtg catgcctatc gggaacatgt ggacgcggcc
     2461 atgcgccgct ttgtcggtga ggcaggcgcc aagacctggg aggtggcagg accgctggtc
     2521 gagctgggcc tgaaccatga gcagcagcat caggaactga tcctcaccga catcctgcag
     2581 gccttgtcgt gcaatccgtt gctgccggcc tatcagggcg gcgaagcgcc tgcgctgcga
     2641 ttggcagcgg cgacccagcc gctgaactgg atcgggatgc cgggcggcac ggtcaaggtg
     2701 gggcatgccg gggaaggctt tgctttcgac aatgaaaccc cgcggcacga cgtgctgctg
     2761 gcgccgtacc gcatcgccga ctgcctggtg acgtgcggcg agtatgcgca attcatcgcg
     2821 gacggcggct acgagcgcgt gcagctgtgg ctgtccgatg gctgggccgc ggtgcaggcc
     2881 ggcggctgga aagcgcccgt gtactggatc gcccccggcg acgcgcgcgc accggccgcg
     2941 cactggcagg tcttcggcct ggagggcgtg cggcccctgg acccggcggc gccggtgagc
     3001 gcactgagtt tctacgaggc cgcggcctat gccgaatggg ccggcgctcg cctgccgacg
     3061 gagttcgagt gggaggccgc ctgcgacgcg ccgggcatca cccacatgtt gggccaggcg
     3121 tggcagtgga cgcgttcctc gtacgacccg tacccgggct tcaggccgct cgcgggcgcg
     3181 gtgggtgaat acaacggcaa gttcatggtg ggccagctgg tgctgcgggg cagcagccgc
     3241 gccacgccgc agggtcatgc gcggcctacc taccgcaact tctttccccc ggccgcccgc
     3301 tggcagttca cgggcctgag gctggcgaag gatgcggcat gctgacccgc ggcctgatcc
     3361 ggaccctgca ggccggcgac agcgaactgc cgctggccgg catgcccgcc ggcgcgttcg
     3421 cgcacgacat gatgcgggca ctggcatcgc gcccgcgcag catatcgccg aagtattttt
     3481 acgacgcgaa gggctcgcgg ttgttcgatc gcatctgcga gctgccggag tactacccca
     3541 cgcgcaccga gttgcgcatc ctcgctgaca acgcgcacga aatcgcggtg cagatgggtc
     3601 cgcgtgccga aatcgtcgag ttcggcgccg gctcgctgcg caaggtgcgg ctgctgctcg
     3661 acgcgatgga tcggccggcg cgctacctgc cgatcgacat ctcgggcgag cacctcgcgc
     3721 attccgccac tgcgctgcag cgggactatc ccggcctgga cgtgcaaccg gtggtggcgg
     3781 attacacgca gcggctgttg ctgcctgcgc gtgtgccggg ggcggggcag cgcgtcggat
     3841 tttttccggg ctcgaccatc ggcaatttca cgccgcaaga ggcgctgcat ttcctgcagg
     3901 tcgcggggca ggtgctgcgc ggcggcgcgc tgctgctcgg ggccgacctg gtcaaggacc
     3961 cggccgtgct gcatgcggcc tacaacgacg ccgagggcgt gacggcggcc ttcaacctga
     4021 acctgctgga gcgcgccaac cgggagctgg gcacgcgttt cgccctggac cagttcgcgc
     4081 acagcgcctt ctacaatgcg ccgcaacagc gcatcgagat gcacctggtc agccggcgcc
     4141 ggcagaaggt cgagttgggg ggcgattgct acgagctgga ggagggcgag acgctgcaca
     4201 cggaaagctc gcacaagttc acggtggagg gtatgcgtgc tctggcaatt ggggcaggat
     4261 ttggccctgg gcccgtatgg accgacgccg acaggctttt cagcgtacat tggctgcagg
     4321 caccgagctg aatatctttg gggattggga aatcatgaaa gccacctgga acggcacgct
     4381 gatcgcgcaa agcgacgaca cggtggtcat ggatggaaac cactactttc cggccgcatc
     4441 gctcaatcgc gaatacgtca ctttcagcaa tcatcacacc agcaacccct tgaagggtga
     4501 agcgagctat tactcgctgc tcgtgaacgg cgagctgaag gctgatgccg tgtggtacta
     4561 cccggacccg aaacctgaag tagaaatgct caaggatcgc gtggcgttcg gaaacggcat
     4621 caagatcgag ccgtaggaag gccctgcctc ttcgacggga gcctgctggc acaagtttgc
     4681 agcctttgta agggctggac ctggggcggc aaagtcggag ccgggcgcgc tttgcgcgct
     4741 tcgtgaactg gctcgtcgcg gttgtttgaa cgaagggccc ttcaaggccc gtagtgagtt
     4801 ctgcgacgcc gaccccaggc cgagtagcgc agcgaagtcg gagcgaagcg cagaccgcca
     4861 cagtgtgagc ccccgccggg taccgcctgc cgcgacccca tgtccagcac gcggcgcggc
     4921 ggcacgttaa gctttgcatc cccggtttgc tcctgcgcga cacattgcgt tttcctctac
     4981 ccatgatcct tgctcccacc ttcacctcgc ccggcgaaat gcccaccctg ctgcgcagcc
     5041 agggctatgc cgtgctcggg ccgccggcgg t
//
LOCUS       DASWIY010000439         3689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_676192, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000439 DASWIY010000000
VERSION     DASWIY010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676192"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VGA59_15655"
     CDS             complement(<1..865)
                     /locus_tag="VGA59_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEX9721153.1"
                     /translation="MNIKSAPFTVWYIFGAVIALLVVQSLFNAEHTQTLTYSQFKSLL
                     RAGKLQEVVVGDDMITGTLDSNGLEALLPKDALARLRESETKPGPHRFATVRVQDPQL
                     VQELEAAKVNFSAKPKQGLLAMLLPWIVPAFLFYVLWHGLNKGMAGAHGMMDIGKSKA
                     KVFMEKQTGVTFEDVAGIEEAEDELREVVEFLKSPERYRRLGGHIPKGVLIVGAPGTG
                     KTLLAKAVAGEAGVPFFSISGSEFVEMFVGVGAARVRDLFEQAKRHAPCIIFIDELDA
                     LGKARGISPIVG"
     gene            1522..1884
                     /locus_tag="VGA59_15660"
     CDS             1522..1884
                     /locus_tag="VGA59_15660"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HEX9721154.1"
                     /translation="MRSKGELLEELKQVLQSMIQGSLSEITRQCGDPACACSHDPARR
                     HGPHLYLKFNAEGRTHSVYVPPEQGQAIKGAHAAWLRFQEIGVQVSAGNRARWLRGLE
                     RTKQAARAKKAKLRSKRT"
     gene            1890..3296
                     /locus_tag="VGA59_15665"
     CDS             1890..3296
                     /locus_tag="VGA59_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057901383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEX9721155.1"
                     /translation="MCRRQLDFGDGLIVEEVGELWEDWMRQADAVLADAQLVNSVYEA
                     LARRWPNSRTRGRKGTPAEVVLRLLLLKHMRNWSYAVLEREVRANLVYRQFTRVGAHK
                     VPDAKTLGKLGLALGPEIIEQLHQRVVAIAQEKGIVAGRKLRVDTTVVETNIHYPTDS
                     TLLGDGVRVLTRAMRRIAQLCAAAGAKLRDRTRSVTYRLLEIGRASRSLGEPGQQRLQ
                     RAYRRLLQASARVVGQAKRFAQEVAEGVKRAASARDQAAVEASRRYLETMIPRVRQVM
                     RQTRSRIVQGNAHAAGKLVSLFEPHTEVIRKGKAGKPTEFGKLVKIQEAEQQIITHYQ
                     VYEQRPSDAQLLIPALHAHQSQLGCTPRLVTADAGFFSADNDSAAHALGVKRVAIPNR
                     STKSAQRKKLQKQRWFRNAQRWRTGSEGRISVLKRRHGMDRCRYKGQRGMQRWVGLGV
                     ISDNLINIGRALAASGQA"
     gene            complement(3463..>3689)
                     /locus_tag="VGA59_15670"
     CDS             complement(3463..>3689)
                     /locus_tag="VGA59_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721156.1"
                     /translation="KLCKVLKAWNSRMKDVEQIVAAVPSIRTEQAGIKNELAEWNSRF
                     DGASEVLAELLQSEDLPNQRPPTFDAPPDP"
BASE COUNT          656 a         1208 c         1199 g          626 t
ORIGIN      
        1 ctccgacgat ggggctgatg ccgcgcgcct ttcccagggc atccagctcg tcgatgaaga
       61 tgatgcaggg agcatggcgt ttggcctgct cgaagagatc ccgcacccgt gccgccccca
      121 cgccgacgaa catctccacg aactccgagc cgctgatgga aaagaacggg accccggcct
      181 ccccggcgac cgccttggcg agcagtgtct tgccggttcc cggcgcgccg acgatcagca
      241 cgcccttggg gatatgtccg cccagccggc ggtagcgctc ggggctcttg aggaactcca
      301 ccacctcgcg cagctcgtcc tcggcctctt cgatcccggc cacgtcctcg aaggtcacgc
      361 cggtctgctt ctccatgaac accttggcct tgctcttgcc gatgtccatc atgccatgcg
      421 ccccggccat gcccttgttc aagccgtgcc acagcacgta gaacaggaag gccggaacga
      481 tccacggcag gagcatcgcc agcagcccct gtttcggctt cgccgaaaaa ttgaccttgg
      541 ccgcctccag ttcttgcacc agttgagggt cttgcaccct taccgtcgcg aaccgatggg
      601 ggccgggttt cgtctcgctc tcgcgcaggc gcgccagcgc gtccttgggc agcagcgcct
      661 cgagtccatt actgtcgagg gtacccgtga tcatgtcgtc gccgaccacc acctcctgca
      721 gcttgccggc gcgcagcagc gacttgaact ggctgtaggt gagcgtctgc gtgtgttcgg
      781 cattaaacag gctctgtacg acgagcagcg cgatgaccgc accaaagata taccagacgg
      841 tgaacggcgc ggacttgatg ttcatggtgt ttccggcgat acgacctgca tatggcgaga
      901 cccacggtcc cgcttcaccg gcgcgccgga ggacagcatt ccgccggctg cgcgtccgtg
      961 tgcagtcgcc ggtaaggctt catgggcctg gcccattcat cgagaacagc tgctgcgttg
     1021 gcactacctg ggcgatgcga aatgtttgac tgcatcgtcg accatccgct gtgtacacca
     1081 tcgcgcatag taccggccaa cagacggccc agccacccct gggagctgag ctcgccttgc
     1141 gcgtagcgtc gctgcaggat ctgcagcggg gtctcgcgcg cccgtccccg cggcacgggt
     1201 gtgagcaacc cgaacacgac ggccgggagc tcgccaatct tcaacaaccg gccgtgcccg
     1261 cccaggtacc gataggttgc ccacgttaga cctgtgcgca agtgtcaggt caactggcgt
     1321 gaggtgcggc gcgcttcccc agagcgtagc tactttccgg cgcaaaaatc ccggcccccc
     1381 gcggagtgac gcgacgcggg cggaggggga tgttttgtgc aagatcgtac caagttcgac
     1441 gcggccgcgg agcccgggtc gagtgaacaa tttcgtcccg acctattgac tggcgcggtg
     1501 tttttgtgta ccttaaccgg tatgagaagt aagggtgagt tgctcgaaga gcttaaacag
     1561 gtcctgcaat ccatgatcca gggttcgctg tccgagatca cccgccagtg cggcgatcct
     1621 gcgtgcgcct gctcccacga tccggcgcgc cgacacggcc cgcacttgta tctgaagttc
     1681 aacgccgagg gcaggaccca ttcggtgtac gtgccccccg agcaggggca ggcgatcaag
     1741 ggcgcgcacg cggcctggct gcgctttcag gagattggcg tgcaggtctc ggcgggcaat
     1801 cgggcgcgct ggctgcgcgg gctcgagcgc accaagcagg cggcccgggc caagaaggcc
     1861 aagctgcgga gcaagcgcac gtgatcgaag tgtgccgccg ccagctcgac ttcggcgatg
     1921 gtctgatcgt cgaggaagtg ggcgagttgt gggaagactg gatgcggcag gccgacgcgg
     1981 tgctcgccga tgcgcagctg gtcaacagcg tctacgaggc gctcgcgcgg cgctggccca
     2041 acagccgcac gcgcgggcgc aagggcaccc cggccgaggt ggtgctgcgc ctgctgctgc
     2101 tcaagcacat gcgcaactgg agctatgcgg tgttggagcg cgaggtgcgg gccaacctgg
     2161 tgtatcgcca gttcacccgc gtgggggcgc acaaggttcc cgacgccaag accctgggca
     2221 aactgggact ggccctgggc cccgagatca tcgagcagct gcatcagcgg gtggtggcca
     2281 tcgcacagga gaagggcatc gtggcgggcc gcaagctgcg ggtggatacc acggtggtgg
     2341 agaccaacat tcactatccc accgacagca cgctgctggg cgacggggtg cgggtgctca
     2401 cccgggcgat gcggcgcatc gcccagctgt gcgcggccgc gggggcgaag ttgcgcgacc
     2461 gcacgcgcag cgtgacctat cggctgctgg aaatcggccg ggccagccgc agcctgggcg
     2521 agccgggcca gcagcggctg cagcgggcct atcggcggct gctgcaggcc agcgcccggg
     2581 tggtggggca ggccaagcgc tttgcgcagg aagtggccga gggggtcaag cgcgccgctt
     2641 cggcccggga ccaagccgcc gtggaagcca gccggcgcta cctcgagacg atgattccgc
     2701 gcgtgcgcca agtgatgcgc cagacgcgca gccgcatcgt ccaaggcaat gcccacgccg
     2761 cgggcaagct cgtgagcctg ttcgagccgc acaccgaggt cattcgcaag ggcaaggccg
     2821 gcaagcctac cgagtttggc aagctggtca agatccagga agccgaacag cagatcatca
     2881 cccactacca ggtgtatgag cagcgcccca gcgacgcgca gctgttgatc ccggccctgc
     2941 acgcacacca gagccaactg ggatgcacgc cgcgattggt caccgccgat gcggggttct
     3001 tctcggccga caatgacagc gccgcccacg ccctgggggt caagcgcgtg gccatcccca
     3061 accgctccac caagagcgcg cagcgcaaga agctgcagaa gcaacgctgg ttccgtaacg
     3121 cccagcgctg gcgcaccggc agcgagggtc gcatcagcgt gctcaagcgc cgccatggga
     3181 tggaccgctg tcgctacaaa ggccagcgcg gcatgcagcg ctgggtgggc ctgggggtga
     3241 tcagcgataa cctgatcaac atcggccgcg cgctcgccgc ttcaggccag gcctgaagct
     3301 tcctccgctt cctccgcttc ctcgacccca gctggactaa ttcagccctc cccgtgagag
     3361 gccccggtgt gccatttatg cgtcgcccgg ccggatccca gaaaaggcat ttttgcgccg
     3421 gaaagtagct agagtcctat gggtcaggag gtgcatctag ctctatgggt caggaggtgc
     3481 atcgaatgtg ggtgggcgct ggttcggcag atcttctgat tgcagaagtt cagccagcac
     3541 ttcagatgcg ccatcgaaac gtgagttcca ttccgcaagc tcgttcttga ttccagcttg
     3601 ctctgtccga atgctcggaa cagcagccac aatctgctcc acgtctttca ttctggaatt
     3661 ccatgccttt agcactttgc acagcttgc
//
LOCUS       DASWIY010000440         4239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3270745, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000440 DASWIY010000000
VERSION     DASWIY010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3270745"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VGA59_15675"
     CDS             <1..330
                     /locus_tag="VGA59_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721157.1"
                     /translation="VRDGQNSLCASCHKVSLKEHTTKSVGTAHTDKIACVDCHMTKTM
                     QTGAGLGKGIAGSDGKNYWMNDITGHLFDVPRITNKAVKGVEPGKAMPTPYTNACGSS
                     CHKAEKM"
     gene            complement(438..2192)
                     /locus_tag="VGA59_15680"
     CDS             complement(438..2192)
                     /locus_tag="VGA59_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9721158.1"
                     /translation="MKSELLPTRHGGDRVADALRAHGVRCLFTLCGGHISPILSASKA
                     RGIRVVDVRDEATAVFAADATARLSGLPGVAAVTAGPGITNTITALKNAQLAQSPVIL
                     LGGAAPTALQGRGALQDIDQRPLVAPHVKLFRKIERVRDLGPAVEEAFEVASSGVPGP
                     VFVECPVDLLYDESSIRQWYAEAAGKGKSMADRALRFYLNRHVRKMFEGSEAAGVQRV
                     LPVAVPRASAGVLNSALAAIARAERPLLVIGSQAVVLSAQADALALAVARLGVPVYLS
                     GMARGLLGRDHPLLLRHQRRDALRESDCVLLAGVPCDFRLDYGKHVRRDAKLIAANRS
                     TRDARLNRKPDIAAIGDAGLFLQDLAQAMPAAATRWEGWLTQLRGRDGERELEIDRQA
                     AAIGEFVNPIAFLRALEQAAGDNALLVADGGDFVGTASYVVHARGPLGWLDPGAFGTL
                     GVGAGFALGAALARPASEVWIVYGDGACGYGLTEFDSFVRHGIPVIAVVGNDAGWTQI
                     AREQVKMLHDDVATVLARTDYHAVAKGFGAEGIVVKQIGEVPAALAKAREFARQGKPV
                     LVNVWLDKTAFRKGSLSM"
     gene            complement(2263..3942)
                     /locus_tag="VGA59_15685"
     CDS             complement(2263..3942)
                     /locus_tag="VGA59_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9721159.1"
                     /translation="MAQDKLILDYVFDHEAQRARQVFLTQPIGGGRVIDYTWADVVGQ
                     ARRMASHLLACKFERGARIAILSKNCAHFFMAELAIWMAGGTTVAIFPTETAETVRFV
                     LEDSEASLLFVGKLDTWPQQASGVPAGLPCIAFPLAPPTSFDTWDAIVARTEPVAGRP
                     HRAPNELAMLIYTSGSTGQPKGAMLPFSSVTRASEGIVDYMKDIAVEGLESRMISYLP
                     LAHSFERAWIECASLVDGSPHIFFAEALDTFVADLQRARPTTFISVPRLWTKFQQGVF
                     TKIPPKKLDRLLGIPIVGRLVGRKVLKGLGLDQVRRAGSGSAPIPAELIAWYRRLGLN
                     LVEGYAMTEDFAFSHSCTATQSAPGYVGVPLPGVQVRLNEDGEILIKSPGQFAGYFKR
                     PELNAVAFTQDGFFRTGDKGERGLDGMLKLTGRVKELFKTTKGEYVAPVPIENKLNIH
                     PMVELSLVSGVGQPAAYAMVVLAEDLRPRVGDPAVRSQVQAELAQLLQDVNRELAAHE
                     RLQMIVVVSEAWSIENGFLTPTMKIRRNRIEAAVTHAVDSWYSTGNPVHWA"
BASE COUNT          705 a         1462 c         1404 g          668 t
ORIGIN      
        1 gtgcgcgacg gccagaattc gctgtgcgcg agctgccaca aggtcagcct gaaggagcac
       61 acgacgaagt cggtcggtac ggcccacacc gacaagatcg cctgcgtcga ctgccacatg
      121 accaagacga tgcagaccgg cgccggcctg ggcaagggca tcgcgggcag cgacggcaag
      181 aactactgga tgaacgacat cacagggcac ctgttcgacg tgccgcgcat cacgaacaag
      241 gccgtcaagg gtgtcgagcc cggcaaggcg atgcccacgc cgtacaccaa cgcctgcggc
      301 agcagctgcc acaaggcgga aaagatgtag gcagtgaagg gagtctgccg gtcgcccctg
      361 cagcgaccga cacccgacgg gctgcatggg agaccatgca gcccgacttc ttttccgaag
      421 tgtgggcggg ccacgagcta catcgacagc gaccctttcc ggaatgccgt cttgtcgagc
      481 cacacattca cgagcaccgg cttgccctgt cgcgcgaact cacgggcctt tgccaatgcg
      541 gctgggactt cgccgatctg cttcacgacg atcccctcgg cgccgaagcc cttcgcgacc
      601 gcgtgatagt cggtgcgcgc cagcacggtg gccacgtcat cgtgcagcat cttcacctgc
      661 tcgcgcgcga tctgggtcca gcccgcgtcg ttgcccacca cggcgatcac cgggatgcca
      721 tgccgcacga agctgtcgaa ttcggtgagc ccgtagccgc aggcgccgtc gccatacacg
      781 atccacacct cgctggctgg ccgcgccagc gcggcgccga gggcgaagcc ggcgcccacg
      841 cccaacgtgc cgaaggcgcc gggatccagc cagccgagcg ggcccctcgc atgcaccaca
      901 tacgaagcgg tgcccacgaa gtcgccgccg tccgccacca gcagcgcgtt gtcgccggcg
      961 gcctgctcca gcgcccgcag gaaggcgatc gggttcacga attcgccgat cgccgcggcc
     1021 tgcctgtcga tttccagctc gcgttcgccg tcacgccccc gcagctgcgt cagccagcct
     1081 tcccaacgcg tggcggcggc gggcatcgct tgcgcgagat cctgcaggaa caggcccgca
     1141 tcgccgatgg cggcgatgtc gggcttgcgg ttcagtcgcg cgtcgcgggt gctgcggttg
     1201 gcggcgatca gcttggcgtc gcggcgcaca tgcttgccgt agtccagcct gaaatcgcag
     1261 ggcacgcccg ccagcagcac gcagtccgat tcgcgcaggg cgtcgcggcg ctggtgccgc
     1321 agcagcaggg gatggtcccg gccgagcaag ccgcgcgcca tgcccgacag gtaaaccggg
     1381 acgccgagcc gcgcgacggc gagggcgagc gcatcggcct gggccgacag caccacggcc
     1441 tggctgccaa tcaccagcag cgggcgctcg gccctggcaa tggcggccag cgcggaattc
     1501 aagacgccgg cgctggcgcg cggtaccgcc accggaagga cacgctgcac gcccgccgcc
     1561 tcgctgccct cgaacatctt tctgacatgg cggttcaggt agaagcgcaa ggcacggtcg
     1621 gccatgctct tgcccttgcc ggcggcctcg gcataccact ggcgaatcga cgattcgtca
     1681 taaagcaggt cgaccgggca ttcgacgaac accggccccg gaacgccgga gctcgcgacc
     1741 tcgaacgctt cctcgacggc gggaccgagg tcccgcacgc gctcgatctt gcgaaacagc
     1801 ttgacgtgcg gtgcgaccag cggccgctgg tcgatgtcct gcagcgcgcc gcgcccttgc
     1861 agtgcggtgg gtgcggcgcc gccgagcagg atgaccggcg attgcgccag ctgggcgttc
     1921 ttgagggcgg tgatggtgtt ggtgatgccc ggccccgccg tcacggcggc gacgcccggg
     1981 aggcccgaca ggcgcgccgt ggcatcggcc gcgaagacgg cggtggcttc atcgcgcaca
     2041 tccaccacgc ggatgccgcg cgccttggac gcgctgagga tcggcgagat gtgcccgccg
     2101 cacagggtga aaaggcagcg cacgccgtgc gcccgcagcg cgtccgcgac gcggtcaccg
     2161 ccatgccgcg tgggcagcag ttccgatttc attgtttttt tccttgggtc ccgttcacac
     2221 gactatagtt tgaacagaga gagagagaga gagagctctg tttcaggccc agtgcacggg
     2281 attgccggtg gaataccagc tgtccaccgc atgcgtgacc gcggcttcga ttcggttgcg
     2341 gcggatcttc atcgtgggcg tgaggaatcc attctcgatc gaccaggcct cgctgaccac
     2401 cacgatcatc tgcaggcgct cgtgcgcggc cagctcacgg ttcacgtctt gcagcaactg
     2461 ggccagctcc gcctggacct gcgagcggac agcggggtct ccgacccgcg ggcgcaggtc
     2521 ttcggccagc accaccatcg cgtacgccgc gggttgcccg acaccggaaa ccagcgacag
     2581 ctccaccatc ggatggatgt tcagcttgtt ctcgatcggc accggtgcca catattcgcc
     2641 ctttgtcgtc ttgaacagtt ccttcacgcg cccggtcagc ttgagcatgc cgtcgaggcc
     2701 gcgctcgccc ttgtcgccgg tgcgaaagaa cccgtcctgg gtgaaagcca ccgcgttcag
     2761 ctcgggccgc ttgaaatagc cggcgaactg gccgggggac ttgatcagga tctccccgtc
     2821 ttcgttgaga cgcacctgca cacccggcag gggcacgccg acataaccgg gcgcgctctg
     2881 ggtcgcggtg cagctgtgcg aaaacgcgaa gtcctcggtc atcgcatagc cttcgaccag
     2941 gttcagcccc agccggcggt accaggcgat cagctccgcc gggatcggcg ccgagccgct
     3001 acccgcccgc cgaacctggt ccaggccgag ccctttgagc accttgcgtc cgaccagccg
     3061 gcccacgatc ggaatgccca gcaaccggtc gagttttttc ggtggaatct tggtgaatac
     3121 gccctgctgg aacttggtcc acagccgcgg caccgagatg aacgtggtcg ggcgtgcccg
     3181 ctgcaggtcc gcgacgaacg tgtccagcgc ctccgcgaaa aatatgtgcg gggagccgtc
     3241 caccagcgac gcacattcga tccaggcccg ctcgaaactg tgcgccagcg gaagatacga
     3301 gatcatccgg ctctccaggc cctcgaccgc gatgtctttc atgtagtcca cgatgccttc
     3361 gctggctcgc gtcaccgagc tgaagggcag catcgcgccc ttgggttggc cggtcgaacc
     3421 ggaggtgtag atcagcatcg ccagttcgtt gggcgcgcga tgcggccgtc cggccaccgg
     3481 ctcggtacgc gccacgatcg cgtcccaggt gtcgaacgat gtgggcggcg cgagcgggaa
     3541 cgcgatgcac ggcaggcccg cgggtacgcc gctcgcctgt tgcggccaag tgtccagttt
     3601 gccgacgaac agcaggctgg cctcgctgtc ttccagcacg aagcgcacgg tctcggccgt
     3661 ttcggtcgga aagatcgcca ccgtcgtgcc gccggccatc cagatcgcca gttccgccat
     3721 gaagaaatgc gcgcagttct tggaaaggat cgcaatgcgc gcgccgcgct cgaatttgca
     3781 ggccagcaga tggctggcca tgcgccgcgc ctggcccacc acgtcggccc aggtgtaatc
     3841 gatcacccgg cccccgccga tgggctgcgt gaggaacacc tgccgtgcgc gttgcgcctc
     3901 gtgatcgaac acgtaatcga ggatcagctt gtcttgtgcc atgttcccgc tccgtggcgc
     3961 ctggagagag gtgcgccgga ctccggcgca cagggtaccc gtggctatcg gtgccgggct
     4021 actggtggat tcccgtgtgt gcgggcaaga tggtgggcgg ccccgtggcg gcaaagtcgg
     4081 ggccgggcgg gccgcggcgc atccacgaag cggtcggcgc tggcgcaccg acttccctca
     4141 cgtttgctct ctcgcgtggc ccgtcgtgga cctcactacg cgcgttgaag cggagggccc
     4201 ttccaggccc ggagtgagtt ctgcgacgcc gacccaggc
//
LOCUS       DASWIY010000441         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_677070, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000441 DASWIY010000000
VERSION     DASWIY010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677070"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VGA59_15690"
     CDS             complement(<1..558)
                     /locus_tag="VGA59_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721160.1"
                     /translation="MTPLDRFLGRFTRLRPGEGRSVVAFFTYALLMMVSYYILKTIRE
                     PLLLTGPSAEIKSYAYAVTALVLLFVIPLYGLVFRHSSKQQLTRYVTVFFVFNLLLFY
                     LAGRLGRDVGFAYYVWVGIFNMMITAQFWAFAADSYNVKSGQRLFPLIMVGATLGGLM
                     APYLAGALFPGIGPWRLMLAAAGLLA"
     gene            729..>1502
                     /gene="htpX"
                     /locus_tag="VGA59_15695"
     CDS             729..>1502
                     /gene="htpX"
                     /locus_tag="VGA59_15695"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF003965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEX9721161.1"
                     /translation="MMRILLFLATNAAVLVLISIVFQVFGLEGILAENGVDLNLQALL
                     VLSAVIGFGGSFISLALSKFMAKRAMGVQVIAQPSSETERWLVETVRRQAAEAGIAMP
                     EVGIFQSPQPNAFATGMRRNSALVAVSTGLLQNMNADEVEAVLGHEVSHVANGDMVTM
                     GLLQGVVNTFVIFLSRIVGHVVDRVVFKTERGYGPAYFITTIVAQIFLSVLASMIVMW
                     FSRRREFRADHGGASLSGRNKMIGALRALQRGQQQDLPGE"
BASE COUNT          279 a          455 c          506 g          262 t
ORIGIN      
        1 ggccagcagg cccgcagccg ccagcatcag ccgccagggc ccgatgcctg gaaacagcgc
       61 gccggccagg tagggcgcca ttaacccacc cagcgttgcg ccgaccatga tcaacggaaa
      121 caggcgctgt ccgctcttga cgttgtaact gtcggcggcg aaagcccaga actgtgccgt
      181 gatcatcatg ttgaaaatcc cgacccagac gtagtaggcg aaaccgacgt cgcggccaag
      241 gcggcccgcg aggtagaaca gcagcaggtt gaaaacgaag aagaccgtga cgtagcgcgt
      301 cagctgctgc ttgctgctgt ggcggaacac caagccgtac agggggatga cgaagagcag
      361 caccagcgcg gttaccgcat aggcgtagct cttgatctcg gccgagggcc ccgtcagcag
      421 cagcggctcg cggatggtct tgaggatgta gtagctgacc atcatcagca gcgcgtaggt
      481 gaagaacgcg acgacgctgc gcccctcgcc ggggcgcagg cgggtgaacc ggcccaggaa
      541 acggtcaagg ggggtcaatc gggttggcca gtgaaccatg gggctgcgct cggctgggtc
      601 gtcgcaggat gacaccacag ggcgctgttg cgcgcaacct gcatcgtcgc gcgtggcctc
      661 ctacttgaaa aacgggtggg acggcagaat ttcttaggcg ttgctcaact cataaggatc
      721 tgcccgaaat gatgcgcatt ctgctgttcc tggccaccaa cgcggccgtg ctggtgttga
      781 tcagtatcgt gttccaggtc tttggcctgg agggcatcct ggccgaaaac ggggtcgacc
      841 tgaacctgca ggccctgctg gtgctgtccg ccgtgatcgg tttcggcggt tccttcattt
      901 cgctggcgct gtcgaagttc atggccaaac gcgcgatggg cgtgcaggtc atcgcgcagc
      961 cgagcagcga aaccgagcgc tggctggtgg agaccgtgcg ccgacaggcg gctgaagccg
     1021 gcatcgccat gcccgaagtg ggcatttttc agtcgcccca accgaacgcc ttcgccactg
     1081 gcatgcgccg caactcggcg ctggtcgcgg tcagcacggg cctgttgcag aacatgaacg
     1141 ccgacgaggt cgaggcggtg ctcgggcacg aggtgtcgca cgtcgccaac ggcgacatgg
     1201 tgaccatggg cctgctgcag ggcgtggtca acaccttcgt gattttcctg tcgcgcatcg
     1261 tgggacacgt ggtcgaccgg gtggtgttca agaccgagcg tggctatggt ccggcctatt
     1321 tcatcaccac catcgtcgcg cagattttcc tgtcggtcct ggccagcatg atcgtcatgt
     1381 ggttctcgcg gcgccgcgag tttcgcgccg accacggcgg tgccagcctg tccggccgga
     1441 acaagatgat cggggcgcta cgcgcgctgc agcgcggtca gcagcaggac ctgccgggcg
     1501 aa
//
LOCUS       DASWIY010000442         7211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1812656, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000442 DASWIY010000000
VERSION     DASWIY010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812656"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1191
                     /locus_tag="VGA59_15700"
     CDS             244..1191
                     /locus_tag="VGA59_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX9721162.1"
                     /translation="MIADNAYSWLTRSAPVAAAQAAGRPLVALESTIIAHGMPYPENV
                     RTAREVETLIRDMGAEPATIALMGGRIRIGLSDDELELLGRSGQVQKVSRRDLPAVLA
                     GGGLGATTVAGTMICAALADIEVFVTGGIGGVHRGAQLSFDISADLQELAKTSVAVVC
                     AGAKSILDIGLTLEYLETHGVPVLSCEQDNFAAFYTRDSGFRADFRLDDAAEQARFIR
                     TKWELGLAGGVVLSAPVPEAAAMARGEIDALTEQALAQAQEQGITGKAVTPFVLARIK
                     SLTGGRSLATNIALVKHNAEVGARLALALARTGRGAGAA"
     gene            1428..2465
                     /locus_tag="VGA59_15705"
     CDS             1428..2465
                     /locus_tag="VGA59_15705"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9721163.1"
                     /translation="MKAAVLNEFGAALTLEERPTPVAGDDEVLIRLEACGVCHSDLHI
                     IDGDQPAFRAATRPRLIPGHEAVGRIVAKGKAVSHLEIGQRVGVAWLYQSCGACEQCC
                     EGRENLCRKSVITGLMVDGGYAEFMLAKASHAIPIPDALGPEEAAPLFCAGVTVYRAL
                     KNAGTAPGQRVAVIGVGGLGHLAVQIARAMGAEVIGLDVSREKLALATELGASQAVDS
                     ADPGALKALAKAGGVHVAIVTSAAKAAFDAAMVVLRPAGTLAVVGLPVQPLTFAALAL
                     VAREIRVIGSAVGTRDDLHAVLALAAEGKLRCRTEPQPLDQINHVLDRMRGGGIYGRV
                     VLRYEDAHSPG"
     gene            complement(2847..3443)
                     /locus_tag="VGA59_15710"
     CDS             complement(2847..3443)
                     /locus_tag="VGA59_15710"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX9721164.1"
                     /translation="MGVQDRDWWRDAQRQKDRDRANLVRELAKASASSAPTMSLALRW
                     GHVGILAFWFAVMGVVYLGMDHYLKPKPLVITAQGDLKIPRHRDGHFYVKGSVNSKPL
                     TFLVDTGASSVTVSEEFARSAGLQGGEPTTFNTANGQLRGRLLRGVAVSAGPFSVSTA
                     TVGVGLVGGRTDHGLLGQSFLSKFQISISKEELVLRRP"
     gene            complement(3905..4498)
                     /locus_tag="VGA59_15715"
     CDS             complement(3905..4498)
                     /locus_tag="VGA59_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9721165.1"
                     /translation="MSRGRPRMFDKEAALDAALRLFWQHGYEGTSIAALAEALKVTVP
                     SLYLAFGNKESLFMLAVERYGHYNGKLYENAFKQKSAREVARSILEGEVQLVLGNGTP
                     DGCLMVHGALATGPGSDTVRSAMAELRRVAEAEVADRFRRAREEGDLPDGQDPESLAA
                     YVMTVASGIAVQARSGLSRAELERVVEIAMGAWPART"
     gene            4581..6119
                     /locus_tag="VGA59_15720"
     CDS             4581..6119
                     /locus_tag="VGA59_15720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9721166.1"
                     /translation="MPPIPERASPREWTGLAVLALPTLLVSMDMTILYLALPSLSAEL
                     HPSGAQLLWISDVYAFVLAAVLIPMGALGDRIGRRKLLLLGSVGFCASSIFAGLASSA
                     EQLILARALLGLSGATLLPSTLAMIRHMFLQPHERTLAIGIWATCFTLGGVLGPLLGG
                     ALLSHFHWGSVFFAAVPIMLVLLLVGPALLPEIREPSSRKVDATSVVQLMLAVLAGTY
                     AMKDAASHGVGWVPAILLVVAVALGAAFLRRQKSVANPLVQVALFRSAAFSTAMSANA
                     LALFAWVGASLLVAQHIQLVLELRAMPAALWMLPSALACMAGCLAAPGLARCWWSGRI
                     ITVGLLLATLGLAVLAASAHRAGLAAIVPAMMMLGFGVSLIVTLSTDIILSEAPPEMA
                     GSASSLSETAADLGGALGVAVLGSVGLAVYRLRVTLPAGMDGGQIASAMDGLSGAVHS
                     AASMPAGTGAWLLDAARSAQGQGLGIATGVGAMLLIVMTFLSWSAIGCTLQQPTEFKG
                     GNNV"
     gene            6112..6504
                     /locus_tag="VGA59_15725"
     CDS             6112..6504
                     /locus_tag="VGA59_15725"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9721167.1"
                     /translation="MSDAAGNENQVRDLIERWVDAVCRGDMDGVLAGHSPEIVMYDVP
                     EPIQCKGLRAYRQTWELFFTENRAGPDRFRLYELEIHAGEDVAVAYGLLAIAGAPARG
                     RLTVGLRRIGRTWAVFHEHHSMPIDLLQ"
     gene            6615..7055
                     /locus_tag="VGA59_15730"
     CDS             6615..7055
                     /locus_tag="VGA59_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721168.1"
                     /translation="MPSIIDLYVSAAVCFAMSCPALAQSEPDEIDQYLAAYMKNVVVV
                     RQVTDTHGQLISCVDIDHQPFAGPARRELSPEGKAMFERGFGPIVPPPPSELCPAGSV
                     AMILPTREQIVYAGGLRRFMSKSPDGGSVAPLQPASGTGSGPRR"
BASE COUNT         1241 a         2284 c         2330 g         1356 t
ORIGIN      
        1 cggtagatgg agggcgcgga ccctgggcag cggcgcgcgg gcagcgaagc gttcgagcag
       61 gaccaggagt tcgtgagcga cgcccatgga gccgtttccg gtcgtcgctt gccgtgcgtc
      121 attcatgtgc aaatgatagc cagggtctta aagccgcgat accagctcgc ctacaggggg
      181 ctcgcagccg agagcggcct tgtgttgcac acttgggtcg accccaccat cgaaggcctc
      241 gaaatgattg ccgacaatgc ctattcctgg ttgacccgca gcgcgccggt ggccgccgcg
      301 caagccgcag gccgcccgct cgtggcgctg gaatcgacca tcatcgccca cgggatgccc
      361 tatccggaga acgttcgcac cgcgcgcgaa gtggagacct tgatccgcga catgggcgcc
      421 gagcccgcga ccatcgcgct gatgggcggg cgaatccgca tcggcctttc ggatgacgag
      481 ctggagttgc tgggccggtc cggccaagtg cagaaagtca gccgccggga cctgccggcc
      541 gtgctggctg gcggcggact cggcgccacc accgtggccg gcacgatgat ctgtgcggcg
      601 ctggccgaca tcgaggtgtt cgtcaccggc ggcattggcg gcgtgcaccg cggcgcgcag
      661 ctgtcctttg acatctcggc ggacctgcag gaactggcca agacctcggt ggccgtggtg
      721 tgcgcgggtg cgaagtcgat cctggacatc gggctcacgc tggaatacct ggaaacacac
      781 ggcgtgccgg tgctcagttg cgagcaggac aactttgccg cgttctacac gcgcgacagt
      841 ggctttcgcg cggacttcag gctggacgac gcggcggagc aggcacgctt catccgcacc
      901 aagtgggagc tgggcctcgc gggcggcgtt gtcctcagcg ctccagtgcc ggaagcggca
      961 gcaatggcac gtggggaaat cgacgcgctg accgagcagg cgctcgcaca ggcccaggag
     1021 caaggaatca ccggcaaggc cgtgacacct ttcgtgctgg ctcgcatcaa gtcgctgacg
     1081 ggtgggcgaa gcttggcgac caacatcgcg ctggtgaaac acaacgccga agtgggggcg
     1141 aggctggcgc tggcgctggc tcgaacgggg cgtggggccg gcgcagcata gccgcctgaa
     1201 cgtccgctgc ccaccgaacc ggctgggctc accagcaccc agcgcatgca cggcctgccg
     1261 cacgtgccaa ctttacgatg gcgaagccgc gaaggcgtca acggcgtcaa cggcgtcgtg
     1321 ccgttgggct tatggcccac cagtgtacgc agcgcgcggc ttgtctcatg gccgcaatag
     1381 atgtgtaatt tcccacgtgg cccttgccgc cacgtcgaga ggccatcatg aaagcagccg
     1441 tcctgaacga attcggcgcc gccctcacgc tggaggaacg ccccaccccg gttgccgggg
     1501 acgacgaagt gctgatacgg ctcgaagcct gcggggtatg ccattccgac ctgcacatca
     1561 tcgacggaga ccagccggcc tttcgcgccg caacaaggcc cagactcatc cccggccacg
     1621 aagcggtggg ccgcatcgtg gcaaagggca aggctgtgtc acatctcgag atcggtcagc
     1681 gcgtgggggt cgcctggctg taccagagct gcggcgcctg cgagcaatgc tgcgaaggcc
     1741 gtgaaaacct gtgccgcaag agcgtgatca ccgggctgat ggtcgatggc ggctacgccg
     1801 agttcatgct ggccaaggcc agccacgcga ttcccattcc cgatgcgctg gggcccgagg
     1861 aagccgcgcc cctgttctgc gccggggtga ccgtctatcg agcgctaaag aatgcgggca
     1921 cggcgcccgg ccaacgcgtg gccgtgatcg gcgtgggcgg actgggccat ttggcggtcc
     1981 agatcgcgag ggcgatgggc gccgaggtga tcggcctgga cgtttccagg gagaaactcg
     2041 cgctcgcgac cgagttgggc gcatcgcagg ccgtcgactc cgcagaccct ggcgcgctca
     2101 aggcactggc caaggcgggc ggggtgcacg tggcgatcgt gacttcggcc gcgaaggccg
     2161 cgttcgacgc cgccatggtc gtgctgcgcc cggccggaac gctggctgtc gtcgggctgc
     2221 cggtccagcc gctgactttt gccgcgctgg cgctcgttgc gcgtgaaatt cgggtgatcg
     2281 gcagcgcggt gggcacgcgg gacgacctgc atgccgtgct ggccctggca gccgagggca
     2341 aactgcgttg ccggacggag ccgcagccgc tggaccagat caaccacgtg ctcgaccgca
     2401 tgcgcggagg tggcatctac ggccgggtgg tgttgcgcta cgaagatgcg cactctcccg
     2461 gttgactccc cgtcactaga tacggcgcaa gccggaggtc gccccgcctg gcgcgtgaaa
     2521 gtagtcacgg cgcagggcga ggtcagggtg atccacatcg acgtcgcccg cgtgaaagat
     2581 gctattggcc tcgacgcggg tgacgcgaga agacccagcc tgactcaggc acacgtcggt
     2641 tccggggcgc agcggtagta gtcccgcaag ggatcggccg atccaggccg caacccgcaa
     2701 ggcagtctgg cgaccggcgg gcgcgtcggt gtattgcgac ctggcctgtg gaaaacttga
     2761 aaatctctaa gtcagacccg ctgtgtcgga agatcaggcc atcctgtgtg agtggaagcc
     2821 cacaatgcgc tggctcccca acaccctcaa gggcggcgta gaacgagctc ttctttcgag
     2881 attgagatct ggaacttgga gaggaaactt tggccgagca ggccatggtc cgtcctgccg
     2941 ccgacaagcc cgacacctac cgttgcggtg gatactgaaa agggaccggc cgaaaccgcc
     3001 actcctcgaa gcagtcgccc gcgtagctgt ccattggcag tattgaaggt cgttggctcg
     3061 ccaccctgca gaccggcact cctggcgaat tcctcactca cagtgacact tgaggcgccc
     3121 gtgtcaacca gaaatgtcaa cggcttactg ttgacggagc ccttcacata gaaatgtcca
     3181 tctcgatggc gagggatttt gaggtcgcct tgagcagtga tgaccagtgg ctttggcttc
     3241 aggtagtgat ccatgcccag atagacaacg cccatcacgg cgaaccagaa cgcgaggatg
     3301 cccacatgac cccacctgag tgctagcgac atcgttgggg cagaagacgc gcttgccttc
     3361 gccagttccc gcacgaggtt tgctcggtca cggtctttct ggcgttgcgc gtcccgccac
     3421 cagtcacggt cctgcacgcc catggtttcc cccttatccg gaacagtgcc cagacgacct
     3481 gagccatcga cgggatgata gacggttcgt cgtggcgacc ggtgctcaag gtgtcggcgt
     3541 gacggccgag cccacgaact cgctgcagcg gccagcgcgg accgccgcat gaccgcggac
     3601 acgcttgaaa acaaatgcgc gtgcggcggg agggcggctt ctggccgaaa gcagatatca
     3661 cgaggtggct catcgttgag tagcgactag cattggatgc aagcgccgcg cgtggaaagt
     3721 cgccgcggga tgcaggtggc cgcctggact gaccgattag ccggcgctgc taccttcatc
     3781 ttgccgatca gaccgtacat gtttgttcct gctttcgttg aggcataaga agaaccgacg
     3841 ataccaatag cagctggtcc cgcggatgct acaaagtctc gacgagtgcg agggcctgtt
     3901 cgcatcacgt tcgggcgggc caagcgccca tcgcgatctc caccacgcgt tcgagttccg
     3961 cccggctcag cccgctgcgc gcctgaaccg cgattcccga cgccaccgtc atcacgtagg
     4021 ctgcaagaga ttccggatcc tggccgtcgg gaaggtcgcc ttcttccctc gctcggcgga
     4081 agcgatccgc gacttcggcc tcggccacgc ggcgcaactc cgccatcgcc gatctgaccg
     4141 tgtccgatcc gggtccggtc gcgagggccc cgtgcaccat caggcaaccg tcgggcgttc
     4201 cgttgcccaa gacgagctgc acctcgcctt ccaggatgct tcgtgcgact tcgcgcgccg
     4261 acttctgctt gaacgcgttc tcgtacagct tgccgttgta gtgcccatac cgctcgacgg
     4321 ccagcatgaa aaggctctcc ttgtttccga aagcaaggta gaggctcggc acggtcactt
     4381 tcaaagcctc cgccaaagcg gcgatcgagg tgccctcgta gccgtgctgc cagaagaggc
     4441 gtaaggcggc gtccagcgcc gcctccttgt cgaacatccg cgggcgtcct cggctcattt
     4501 gtatcgatcg ctataaaaag attgacgaaa ggggtccgac ttattataac ggtcactaca
     4561 ttaatcacga aaggacgcaa atgccgccaa tccccgaacg ggccagccca cgcgaatgga
     4621 cgggacttgc tgtccttgcg ctcccgaccc tcctggtctc gatggacatg accattctct
     4681 acctggcact gccgagtctg agcgcggagc ttcatcccag cggtgcgcaa ctgctgtgga
     4741 tttccgacgt ttacgcattc gtccttgctg cggtgttgat cccgatgggt gcgctcggcg
     4801 accggatcgg acgtcgcaag ctgctccttc tgggatccgt gggtttttgc gcgtcctcga
     4861 tctttgccgg gttggcctct tcggccgagc agctcatcct ggcgagggcg ctcctggggc
     4921 tttccggcgc aacgctcctt ccttcgacgc ttgcgatgat tcgccacatg ttcctgcagc
     4981 cccacgaacg caccctggcc atcggcatat gggcgacatg cttcacgctg ggaggcgtgc
     5041 tggggccgct gctgggtggc gcactgcttt cgcacttcca ctggggatcg gtctttttcg
     5101 cggccgtccc catcatgctc gtgctcctgc ttgtaggccc agccctgctt ccggagatcc
     5161 gggagccttc ctctcgcaaa gtcgacgcga caagcgtggt tcagctgatg cttgccgtat
     5221 tggcgggtac atacgccatg aaggatgccg caagccacgg ggtcggctgg gtaccggcga
     5281 tcctgctagt cgtcgcggtg gctcttggcg cggcattcct gcgccggcaa aaatctgtcg
     5341 ccaaccctct tgtgcaggtt gcgctctttc gcagtgctgc cttcagcacg gccatgtcag
     5401 ccaatgcact ggcgctgttc gcatgggtcg gcgcctcact gctggtggcc cagcatatcc
     5461 aattagtttt ggagctgcgc gccatgcctg ccgctctgtg gatgcttccg agtgctttgg
     5521 catgcatggc aggttgcctg gcagcacctg gcttggcgcg ttgctggtgg agcggaagga
     5581 tcatcaccgt cggccttctt cttgcaacgc tcgggctggc agttctggcc gcgtcggctc
     5641 accgagctgg tctggctgcg atcgtgccag ccatgatgat gctgggtttt ggcgtgtcgc
     5701 ttatcgtgac gctctccaca gacatcatcc tcagcgaggc ccctccggaa atggcgggct
     5761 ccgcatcttc gctttccgaa acggcggcgg acctcggcgg cgcactcgga gtggccgtgc
     5821 ttggcagcgt agggctggcg gtctacagac tccgcgtgac gctgcctgcc ggaatggacg
     5881 gtgggcaaat agcgtccgca atggatggcc tctccggcgc ggtgcacagt gccgcatcca
     5941 tgccggcggg cacgggcgcc tggcttctgg acgctgcccg ctcggcgcag ggccaagggc
     6001 tcgggattgc cactggggtt ggcgccatgc tacttatcgt catgacattc ctgagctggt
     6061 cagcgattgg ctgtaccttg caacagccca cagaattcaa gggtggcaac aatgtctgac
     6121 gccgctggca acgagaatca ggtccgcgac ctgatcgaac gctgggttga cgctgtgtgc
     6181 cggggcgaca tggatggcgt gcttgccggc cactcgccag aaatcgtcat gtacgacgtg
     6241 ccagaaccca tccagtgcaa aggactgcgc gcctaccggc agacttggga attgttcttt
     6301 acggagaacc gggccgggcc tgatcgcttc cggttgtatg agttggagat tcacgctggc
     6361 gaagatgtcg ctgtcgcgta tggcttgctt gcaatcgccg gagcgcctgc tcggggcagg
     6421 ctcacggttg gactacggcg aattggacgc acttgggcag tctttcatga acaccactcg
     6481 atgcccatcg atcttctaca gtagtacgcc cgctgagatc ttggcgtctg cttgtggccg
     6541 aacgcagacc tcgtgccgtg gcccgcttgg acaatctatg attcggcgac caccacgggc
     6601 ccgaccgctc gcttatgccc tccatcatcg atctatacgt gtcggcggca gtttgtttcg
     6661 caatgtcttg cccagcgctt gcgcagtctg aacctgacga gattgaccag tatctcgcgg
     6721 cgtacatgaa gaacgtggtc gtcgtgcggc aagtgacgga cacgcatggg cagttgatca
     6781 gctgcgtgga catcgaccac cagccttttg caggtccggc gcgtcgcgaa ctttcgccgg
     6841 agggaaaggc aatgttcgag cgaggcttcg ggcccatcgt cccaccccca ccttcggagc
     6901 tttgccccgc gggcagcgtc gcaatgattt tgcccacgcg cgaacagatc gtctatgcag
     6961 gtggcctgcg gcggttcatg tcaaagagtc ccgatggcgg ctcggtcgcg cctttgcaac
     7021 cggccagcgg cacaggttct gggccacgtc gctagagttc tcggcttcgg attgacgcca
     7081 gcggcacgga acaccggcgc ttcagcttgg cgtcccgcgc gggatttccc tttatggcag
     7141 ctatcggcca ggagcagccc tcacgacgat ccgacataat ttccgcatgt ctggctcgcc
     7201 gcccccaatc g
//
LOCUS       DASWIY010000443        12449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3608716, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000443 DASWIY010000000
VERSION     DASWIY010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12449
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3608716"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..747)
                     /locus_tag="VGA59_15735"
     CDS             complement(94..747)
                     /locus_tag="VGA59_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX9721169.1"
                     /translation="MLRLTVLLLLLANAAYFAWSQGLLAPWGLAPVQQSEPQRLQQQI
                     KPQALHILGSEEARRFDAAVSATRAQECLQAGVFEEGRIASLKQALEPWPAGTWTLEP
                     AVEPARWIVYMGKFPSAENANRQKAELRQIGVSFEALADPSLEPGLSLGGFATEAAAT
                     QAMDALSQRGVRNAKVLRERAELRGLLLKLPAVDDSLRPRLEVLAAVLGGRNLRPCR"
     gene            complement(757..1524)
                     /locus_tag="VGA59_15740"
     CDS             complement(757..1524)
                     /locus_tag="VGA59_15740"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX9721170.1"
                     /translation="MSTDWPSQGIGAAVSPLLPGFTVEVVPEIASTNTELMQRARAGR
                     VEPVLLVAEHQTAGRGRLGRGWVSKAGQSLTFSLGLPLAPADWSGLSLAVGVSLAESL
                     HPGIQLKWPNDLWLADRKLAGVLIETASFGESGVAGRYAVIGVGINIAARDETGLATA
                     PASLQELLPAVSPGEVLLRIATPLVQAVQAFETFGFAAFQARFNARDALRDRAVVLSD
                     GTTGTAHGASEAGALLVHTAGGMKTVTSSEVSVRPAA"
     gene            complement(1521..2063)
                     /locus_tag="VGA59_15745"
     CDS             complement(1521..2063)
                     /locus_tag="VGA59_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX9721171.1"
                     /translation="MAEKATTGRYIEAPLQGAASRPGSGPDAPFRRIQVRRSGVHGKG
                     VFALQDIAEGETIIEYVGEVITWKEALRRHPHDPQDPNHTFYFHVDERRVIDAKCGGN
                     SSRWINHSCDPNCEADEEDGRVFIKALRNIRAGEELNYDYGLIIDERYTKKLKADYPC
                     WCGAANCRGTLLAPKRRQRK"
     gene            2180..5299
                     /locus_tag="VGA59_15750"
     CDS             2180..5299
                     /locus_tag="VGA59_15750"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEX9721172.1"
                     /translation="MPKTLIIAEKPSVAQDIVRALTPLAGKFDKHDEYFENEKYIVTS
                     AVGHLVEIQAPEEFDVKRGKWSFAHLPVIPPYFDLKPVDKTRSRLNAVVKLARRKDVG
                     DLVNACDAGREGELIFRLIEQFAGGGKPLGKPVKRLWLQSMTPQAIREGFEQLRSNKQ
                     MEGLANAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLAVVVEREEQ
                     IRKFVSRDYWEIHATFLAEAGEYPAKWFDPQWKKNPDDAEIKADRVWSEREAMAIADA
                     VRGKAASVTEESKPTTQASPLLFDLTSLQREANGRFGFSAKTTLSLAQSLYERHKALT
                     YPRTDSRALPEDYPPVVKKTMGMLATSGMRHLAPFAKQAIDENYVKPSKRIFDNSKVS
                     DHFAIIPTLQAPSGLSDAEQKLYDLVVKRFLSVFFPSAEFMVTTRITTVAPTLAGKPA
                     ALPPEGAMPGLGRPGAPAVIGHSFKTEGKVLVKPGWLAIWGKEAADEVADAKEGDKGQ
                     ALVPVKPGEMVRTETIEPKGLKTRPPARYSEATLLGAMEGAGKLVEDDELREAMQEKG
                     LGTPATRAAIIEGLITERYMFREGRELIPTAKAFQLMTLLRGLGVEELSKPELTGEWE
                     FKLAQMEHGKLSREAFMREIGEMTKHIVKKAKEYDRDTVPGDYATLGTPCPNCGGVVK
                     ENYRRYACIGRPAGMADTDAAGKAGEADTQGCGFSFGKTPAGRTFEIEEVEQLLRDKK
                     VGPLEGFRSKAGWPFTAEIVLKYNEEEKNWKLEFDFGEDRGAAETGEIVDFSGQESLG
                     PCPKCGGRVFEHGSNYVCERSVPTGQQPTPTCDFKSGQIILQQPVAREQIAKLLQAGK
                     TDLLDKFVSMRTRRPFKAFLAWNKEQDKVIFEFEPRASKFPPRKSAAGKTPAAREPAA
                     KYATDKTAAKTAAKKAPSTAAKASPAKKPAPAGKAAPAGKVAAAKAPRKTAAGLKPSA
                     ELAAVIGGEPVARTQVIKKLWDYIKANGLQDAKDKRSINADAKLLPVFGKPQVTMFEL
                     AGVVGKHLS"
     gene            5386..5895
                     /locus_tag="VGA59_15755"
     CDS             5386..5895
                     /locus_tag="VGA59_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX9721173.1"
                     /translation="MTSTGFSLASPDIQANALIDKKFEFDGFGCSGENKSPALQWSDA
                     PADTRSFAVTVYDPDAPTGSGFWHWMVVNIPGNATGLAKNAGAIGGANLPKGAKHVRI
                     DYGIFGWGGPCPPPGDPPHRYVFTVHALKAERLDVPDDATAAVGGFMINANTIAKASF
                     MAKYGRPAA"
     gene            complement(6065..7420)
                     /locus_tag="VGA59_15760"
     CDS             complement(6065..7420)
                     /locus_tag="VGA59_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX9721174.1"
                     /translation="MSRFIAVDRDTAYLLPPSVDEWLPEDHLARFVVEVIDQLDLSEL
                     VRQYAGRGSDAHHPAVLLGLLIYGYAGGVFSSRKIERATYDSVAFRYVAANTHPDHDT
                     IATFRRRFLPQVKSLFVQVLMLAREMKCLKLGTIALDGTKVHANASKHKALSWAHANK
                     IEAQLRGEVQALLDLGEKADRAAVPDGMDVPAEIARREQRLKAIDAAKAQIEQRARER
                     FEVEQQEHQAKLQRRQAQRDAGKKPRGKDPEPPQAGPKDSDQVSLTDADSRIMPVSGG
                     GFEQSYNAQAAVDTDTMLVIATHVSQAPNDKREIAPILDELGALPKPLGQVNTLLADT
                     GYCSQANVVHCHASQIDPMLAMRRDSHHLAVLERFAPDAPVPESDDPVVQMAHRLKTK
                     AGRVLYGLRKQTIEPVFGIVKQVMGWRQMSMRGLDKAEGEWSLVTLAWNVKRLHVLRA
                     A"
     gene            complement(7502..8368)
                     /locus_tag="VGA59_15765"
     CDS             complement(7502..8368)
                     /locus_tag="VGA59_15765"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEX9721175.1"
                     /translation="MKRQDFRFFHRLQVRWAEIDMQKIVFNAHYLMYVDTAVAGYWRA
                     LALPYEEAMHMLGGDTYVKKATVEFHASARMDDRLDVALKCGRIGNSSMNFTSAIFRG
                     DELLIACELIYVFADPETQTSRPVPPLLRDVLTGYEAGEPMVQIKTGRWAELGDEARR
                     IRTEVFLQEQRIPAEMEWDEGDAAAVHAVAYNRLGQAVATGRLLQQRPGIARIGRMAV
                     HRALRGSGVGRQVLQALLSAATARGDREAMLHAQRSAEDFYLGLGFAPRGEAFDEAGI
                     AHIEMVRSLAGP"
     gene            8516..9652
                     /locus_tag="VGA59_15770"
     CDS             8516..9652
                     /locus_tag="VGA59_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX9721176.1"
                     /translation="MAFIYYVTQIQFDFGAVNLLKQECERVGIKRPLVVTDPGVKAAG
                     VLRKALDALTGIPVAVFDQTPSNPTAAAVRAAVEVYKANGCDGLVAVGGGSAIDCAKG
                     VAIAATHEGPLTRYATIEGGSPRITDKVAPLIAVPTTSGTGSEVARGAIIIVDDHRKL
                     GFHSWHLVPKTAICDPELTLGLPPKLTAATGMDAIAHCMETFMSAAVNPPADGIALDG
                     LERGWANIERATRDGSDREARFNLMSASMEGAMAFQKGLGCVHSLSHSLGGVDPRLHH
                     GTLNAVFLPAVVRFNAQAESVRKDRRLERMAHAMDLRSAADIPDAIRDMNARLGLPDG
                     LAAMGVRRDQFEEVIKGALADHCHKTNPRLASSRDYEDMLAASM"
     gene            complement(9680..12010)
                     /locus_tag="VGA59_15775"
     CDS             complement(9680..12010)
                     /locus_tag="VGA59_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEX9721177.1"
                     /translation="MQKIIRRIAAEIRVHEHQVQAAVDLFDGGATVPFVARYRKEATG
                     GLDDIQLRELDYRLGYLRELEGRRSAVLKSIEEQGKLTPVLRAAIEAAPTKQELEDLY
                     LPYKPKRRTKGQIAREAGIEPLADRLFADPSLDPAPEALPFVHAGKGEGGEDFTTVAA
                     VLDGVRDILSERWAEDPALVRQLREWLWSEGLFKAKLMAGKDENNADVAKFRDYFGYD
                     EPIGRVPSHRALAVFRGRQLEILEAKLALPVEPEPGRPSIAEGRIALHLGWSHQGRAA
                     DDLLRKCVAWTWRVKLSLSTERDLFARLREEAEKVAIKVFADNLRDLLLAAPAGPRVV
                     MGLDPGIRTGVKVAVVDATGKLVATATVYPHEPRRDWDGSLHQLELLCRKHGVNLIAI
                     GNGTASRETDKLAADLIKRLDTEPGGLQRVVVSEAGASVYSASEYASQEMPDVDVSLR
                     GAASIARRLQDPLAELVKIDPKSIGVGQYQHDVNQSELARTLEAVVEDCVNSVGVDLN
                     TASVPLLARVSGLSAGVAKAVVRWRETHGAFSNRQQLMEVSGLGARTFEQSAGFLRIR
                     GGENPLDMTGVHPETYPVVERMLAATGKPVAQLMGRAEMLRTLKPELFANEKFGVITV
                     KDILGELEKPGRDPRPDFKVARFNEGVEGIGDLREGMMLEGTVSNVAAFGAFVDLGVH
                     QDGLVHVSQLSHKFVKDAREVVKTGDIVTVKVMEVDVARKRIALSMKLGEAPARRDGP
                     RDNRYEGAGRGQRAPRGEPLNTAIAGAFAKLQGPRK"
     gene            12151..>12449
                     /locus_tag="VGA59_15780"
     CDS             12151..>12449
                     /locus_tag="VGA59_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721178.1"
                     /translation="MRARLFFLVLAILLVAGFAAQNWSEFMRTSPLTFGVVEEQAALG
                     LIMLGALGVALMAFLLSSAMQESRMWLESSRHAKSLQAQRDLAEKAEASRFTELR"
BASE COUNT         2111 a         4208 c         4057 g         2073 t
ORIGIN      
        1 ggttcccttg tagacgttca ctggagccgt tcgcgcgcga tcgcccgaat cggcggcagg
       61 gccgcgggtt cctagcggca gggccgcggg gtcctagcgg cagggccgca gatttcttcc
      121 gccgaggacc gctgcgagta cttccaggcg cggccgcagg ctgtcgtcta cggccggcag
      181 cttgagcagc agcccccgca gctcggcccg ctcgcgcaac accttggcgt tgcgcacgcc
      241 gcgctgcgac agcgcgtcca tcgcctgcgt ggcggccgcc tcggttgcga agccgcccag
      301 cgacaggccg ggttccagcg aagggtcggc caaggcttcg aaggaaacgc cgatctgccg
      361 cagctcggcc ttttgccggt ttgcgttttc ggcactggga aacttgccca tgtagacgat
      421 ccagcgcgcc ggctccacgg ccggctccag cgtccaagtg cccgcgggcc agggctccag
      481 cgcctgcttc aggctcgcga tccggccttc ctcgaacacg ccggcctgaa ggcactcctg
      541 cgccctcgtt gccgacaccg ccgcgtcgaa ccgcctcgct tcttcgctgc ccaggatgtg
      601 cagggcctgc ggcttgatct gctgctgcag gcgctggggc tcggattgct gaaccggcgc
      661 caggccccaa ggcgccagca ggccctgcga ccacgcgaag taggcggcgt tggccaacag
      721 cagcagcagg acggtcaggc gcagcatcgc aagaaatcag gcagcggggc gcacgctcac
      781 ttcggaactg gtcacggtct tcatgccgcc agcagtatgc accagcaggg ccccggcttc
      841 gctggcgccg tgcgcggtgc cggtggtgcc gtcgctgagg acaacggccc ggtcgcgcag
      901 ggcgtcgcgc gcgttgaagc gcgcctggaa ggcggcaaac ccgaaagttt cgaaggcctg
      961 cacggcctgc accagcggcg tcgcgatacg caacagcacc tcgcccggcg acacggcggg
     1021 cagcagctcc tgcaggctgg ccggagcggt agcaagcccg gtctcatcgc gcgcggcgat
     1081 gttgatgccg acaccgatga ccgcataacg ccctgccacg ccggattcac cgaaactcgc
     1141 ggtctcgatc agcacaccgg ccagcttgcg gtcggcgagc cacaggtcgt tcggccattt
     1201 gagctggatg cccggatgca ggctctcggc caggcttacg cccacggcca gcgacaagcc
     1261 cgaccagtcg gcgggcgcca gcggcaggcc gagtgaaaag gtcaaggact ggccggcctt
     1321 gctgacccag ccacggccca gccgcccgcg ccccgcggtc tgatgctccg ccaccagcaa
     1381 caccggttcc acccggccgg cgcgcgcccg ctgcatcagc tcggtgttcg tggaagcgat
     1441 ctcgggtacg acctcgaccg tgaagccggg cagcagcggc gaaaccgccg cgccaatgcc
     1501 ttgggagggc cagtcggtgc tcacttgcgc tgcctgcgtt tgggcgcgag cagtgtgccg
     1561 cggcagttag ccgcaccaca ccagcacggg tagtcggcct tgagcttctt cgtgtagcgc
     1621 tcgtcgatga tcaggccgta gtcgtagttc agctcttcac cggcccggat gttgcgcagc
     1681 gccttgatga acacccggcc atcttcctcg tcggcctcgc agttggggtc gcacgagtgg
     1741 ttgatccagc gcgatgagtt gccgccgcac ttggcatcga tcacccgtcg ctcgtcgacg
     1801 tggaaataaa aggtatggtt cgggtcttgc ggatcgtgcg gatggcggcg cagggcctct
     1861 ttccacgtga tcacttcgcc cacgtattcg atgatggttt cgccctcggc gatgtcctgc
     1921 aaggcgaaca cgcccttgcc gtggacgccc gagcgccgga cctggattcg tctgaagggg
     1981 gcatccggcc cgctgcccgg gcgggatgcc gcgccttgca atggcgcctc gatgtaccgg
     2041 cctgttgttg ctttttctgc catcgccaaa actctgttag tttaaatatg cacacatgcg
     2101 cgcgtgcgcg tgcgcgcgta agagcgagat tgtatgaaca ctttgattcc ggggcgccga
     2161 gcgccacgtt tccaccttta tgcctaagac attaatcatt gccgagaagc catcggtggc
     2221 gcaggacatc gtgcgcgcgc tcacgccgct ggccgggaaa ttcgacaagc acgacgagta
     2281 cttcgagaac gagaaataca tcgtcaccag cgccgtgggc cacctcgtgg aaatccaggc
     2341 gccggaggag ttcgatgtca agcgcggcaa gtggagcttc gcgcacctgc cggtgatccc
     2401 gccctacttc gacctgaagc ccgtggacaa gacccgctcg cgcctgaacg cggtggtgaa
     2461 gctggccagg cgcaaggatg tcggcgacct ggtcaacgcc tgcgacgccg gccgcgaggg
     2521 cgaactcatc ttccgcctga tcgagcagtt cgccgggggc ggcaaaccgc tgggcaagcc
     2581 ggtcaagcgg ctgtggctgc aatcgatgac gccgcaggcc attcgcgagg gcttcgagca
     2641 actgcgcagc aacaagcaga tggaaggcct ggccaacgcg gcgcgctcgc gttccgaggc
     2701 cgactggctg gtcggcatca acggcacgcg ggcgatgacc gcgttcaatt cgcgcgacgg
     2761 gggctttttc ctcaccacgg tgggccgggt gcagacaccg acgctggcgg tggtggtcga
     2821 gcgcgaagag cagatccgca agtttgtcag ccgcgactac tgggaaatcc acgccacctt
     2881 cctggccgaa gcgggcgagt accccgccaa gtggttcgac ccccagtgga agaagaaccc
     2941 ggacgacgcc gaaatcaagg ccgaccgggt ctggtcggaa cgcgaggcca tggccatcgc
     3001 cgacgcggtg cgcggcaagg ccgccagcgt caccgaggaa tcgaaaccca cgacccaggc
     3061 ctcaccgctg ctcttcgacc tgacgtcgct gcagcgcgag gccaacgggc ggttcggctt
     3121 ttccgccaag accacgctgt cgctggcgca aagcctgtac gagcggcaca aggcgctgac
     3181 ctacccgcga accgattcgc gcgccctgcc cgaggactac ccgccggtcg tgaagaagac
     3241 tatgggcatg ctggccacga gcggcatgcg ccacctggcc ccgttcgcca agcaggcgat
     3301 cgacgagaac tacgtcaagc cgagcaagcg catcttcgac aacagcaagg tcagcgacca
     3361 ctttgcgatc atccccacgc tgcaggcccc cagcgggctg tcggacgccg aacagaagct
     3421 gtacgacctg gttgtgaagc gcttcctctc ggtgttcttt ccctcggccg agttcatggt
     3481 caccacgcgc ataacgacag tagcccccac gcttgcgggc aagcccgctg cgctgccccc
     3541 cgagggggcc atgccgggct tggggcggcc cggcgccccg gcggtcatcg gccacagctt
     3601 caagaccgag ggcaaggtgc tggtcaagcc cggctggctc gccatctggg gcaaggaagc
     3661 cgcggacgaa gtagccgatg ccaaggaagg cgacaaaggc caggccctgg tgccggtgaa
     3721 gcccggcgag atggtgcgca cggagacaat cgaacccaag ggactgaaga cccggccgcc
     3781 ggcacgctac tcggaagcca cgctgctggg cgccatggaa ggcgccggca agctggtcga
     3841 agacgacgag ctgcgcgagg ccatgcagga aaaggggctg ggcaccccgg ccacgcgggc
     3901 agccatcatc gaaggcctga tcaccgagag gtacatgttc cgcgaagggc gcgaactgat
     3961 cccgacggcc aaggcgttcc agctgatgac actgctgcgc ggcctgggcg tggaagagct
     4021 gtccaagccc gagctcaccg gagaatggga attcaagctg gcccagatgg agcacggcaa
     4081 gctttcgcgc gaagccttca tgcgcgagat tggcgagatg accaagcaca tcgtcaagaa
     4141 ggccaaggaa tacgaccgcg acaccgtgcc cggcgactac gcgacccttg gcacgccctg
     4201 cccgaactgc ggcggcgtgg tcaaggaaaa ctaccggcgc tacgcctgca tcgggcgtcc
     4261 cgctggcatg gccgacaccg acgcggccgg caaggccggt gaagccgata cgcagggctg
     4321 cggcttctcg ttcggcaaga ctcccgccgg ccgcaccttc gagatcgaag aggtcgagca
     4381 gctgctgcgc gacaagaagg tcgggccgct ggaaggcttc cgctcgaaag cgggctggcc
     4441 gttcaccgcc gagatcgtgc tcaagtacaa cgaggaagaa aagaactgga agctcgagtt
     4501 cgacttcggc gaggaccgcg gcgcggcgga aaccggcgag atcgtcgact tctccggcca
     4561 ggaatcgctg ggcccctgcc ccaagtgcgg cggccgcgta ttcgagcacg gcagcaacta
     4621 cgtgtgcgag cgctcggtgc cgaccgggca gcagcccacg cctacctgcg acttcaagag
     4681 cggccagatc atcctgcagc agccggtcgc gcgcgagcag atcgccaagc tgctgcaggc
     4741 gggcaagacc gacctgctgg acaagttcgt ctcgatgcgc acgcgccggc ccttcaaggc
     4801 gttcctggcc tggaacaagg aacaggacaa ggtgatcttc gagttcgagc cgcgcgccag
     4861 caagttcccg ccgcgcaaat ccgcggcagg caagacgccg gcggccaggg aaccggccgc
     4921 gaagtacgcg acggacaaga cggcggcgaa gaccgccgcg aaaaaggctc cgtccacggc
     4981 agcgaaagcg tcacccgcca agaagccggc gccggccggg aaggccgcac cggccgggaa
     5041 ggtggccgca gccaaagccc cgcgcaagac cgccgccggc ctgaagccca gcgccgagtt
     5101 ggcggccgtg atcggcggcg agccggtggc ccgcactcag gtcatcaaga agctttggga
     5161 ctacatcaag gccaacggct tgcaggacgc gaaggacaag cgttccatca acgccgacgc
     5221 caagctgctg ccggtgttcg gcaagccgca ggtgacgatg ttcgagctgg ccggggttgt
     5281 cggcaagcac ttgagctgaa gccgtcgttg cgcctcaccg gtgagggatg acaagtccgc
     5341 acggttcgta gcatcatggc gttcccttgc tcgaaggaga cttgaatgac aagtaccggc
     5401 ttcagcctcg ccagccccga catccaggcc aatgccctga tcgacaagaa gttcgagttc
     5461 gacggcttcg gctgttcggg cgagaacaag tcgcccgcgc tccagtggag cgacgccccc
     5521 gcagacacca ggagtttcgc cgtgacggtg tacgacccgg acgcgcccac cggctcgggc
     5581 ttctggcact ggatggtcgt caacatcccg ggcaacgcga ccggactcgc gaagaacgcc
     5641 ggcgccatcg gcggcgccaa cctgcccaag ggcgcgaagc atgtgcgcat cgattacggc
     5701 attttcggct ggggcggccc ttgcccgcca ccgggcgacc cgccgcaccg gtatgtgttc
     5761 acggtccacg cgctcaaggc cgagaggctc gatgtccccg acgatgcgac cgcggcggtg
     5821 ggcggcttca tgatcaatgc gaacaccatc gccaaggcct cgttcatggc gaagtacggc
     5881 cggcccgcgg cctaggagcc tgtcgggctt gagtgcgcgc aggttgtgat ttgcggggga
     5941 agcggatttt taggaggccg attcgcgctt tgcgcgtgat tgcacgattg cgcgaagcct
     6001 ggccagtcct tggacgctgt ctttggcgct cgttgggtcg attttctgac cccatttccc
     6061 cgtttcatgc cgccctcagc acgtgcagcc gcttgacgtt ccatgccagc gtcaccaaag
     6121 accattcgcc ctcggccttg tccagcccgc gcatggacat ctggcgccag cccatcacct
     6181 gcttgacgat cccgaacacc ggctcgatcg tttgtttgcg caatccatac agtactcgcc
     6241 ctgctttggt cttgagccga tgggccatct ggaccaccgg atcgtcgctc tctggcacag
     6301 gagcgtccgg cgcgaagcgc tccagtactg ctaaatgatg tgaatcacgc cgcatggcaa
     6361 gcatgggatc gatctggctc gcatggcagt gcaccacatt ggcctggctg cagtagccgg
     6421 tgtcggccaa cagcgtgttg acctgcccca gcggcttggg cagggcgccc agttcatcca
     6481 gtatcggagc gatttcgcgc ttgtcgttgg gcgcttggct cacatgtgtc gcgatcacca
     6541 gcatggtgtc ggtgtccacg gcggcctgcg cgttgtagct ttgctcgaag cccccgcctg
     6601 agacgggcat gatgcgcgag tccgcatccg tcaggctgac ctggtcgctg tccttggggc
     6661 ctgcctgtgg cggctcgggg tccttaccgc gcggcttctt gcccgcatcg cgctgggctt
     6721 ggcggcgttg cagcttggct tgatgctcct gctgttcgac ttcgaaccgt tcgcgcgccc
     6781 gctgctcgat ctgcgccttg gccgcatcga tggccttcaa gcgctgctcg cgccgcgcga
     6841 tctcggcggg cacgtccatg ccatcgggca ccgccgcgcg gtcggccttc tcacccaaat
     6901 ccagcagggc ctgcacttca ccgcgcagtt gggcttcgat cttgttggcg tgagcccatg
     6961 acagggcctt gtgtttgctg gcgttggcat gcaccttggt gccgtccagc gcgatggtgc
     7021 ccagcttcag gcacttcatc tcgcgcgcca gcatgagcac ctgcacgaac aaggacttca
     7081 cctggggcag gaagcgccgc cggaaggtgg cgatggtgtc gtggtcaggg tgggtgttgg
     7141 ccgccacata ccgaaaggcc accgaatcgt aggtggcccg ctcgatcttg cgcgagctga
     7201 acacgccgcc ggcgtaaccg tagatcagca ggcccagcag caccgccggg tgatgcgcat
     7261 ccgagccgcg gcccgcgtac tggcgcacca actcgctcag gtcgagctgg tcgatgacct
     7321 cgaccacgaa gcgtgccaag tgatcttcgg gcagccattc atcaaccgat ggcggcagca
     7381 ggtacgcggt gtctcggtca acggcaatga aacggctcat cgatgggatc tcctcgctcc
     7441 tactgcagca attgtcacgt catgctcgac ttcgcgaaag gcgggaaagc ccgacaggct
     7501 cctagggccc ggccaatgag cggaccatct cgatgtgggc gatgcccgct tcgtcgaacg
     7561 cttcgccgcg aggcgcaaag cccaggccga ggtagaagtc ctcggcactt cgctgcgcgt
     7621 gcagcatggc ttcgcggtcg ccgcgcgccg tggccgcact caacaaggct tgcaacacct
     7681 gacggccgac gcccgatcca cgcagggccc ggtgcaccgc catgcggcca atcctggcaa
     7741 ttcccgggcg ctgctgcagc agccgccccg tcgcgaccgc ctggcccagc cggttatagg
     7801 cgaccgcgtg caccgccgcc gcatcgccct cgtcccactc catttccgcc gggatccgct
     7861 gctcctgaag aaagacttcg gtgcggatgc gcctcgcttc gtcgcccagc tccgcccagc
     7921 gcccggtctt gatctggacc atgggttcgc ccgcctcgta acccgtcagc acatcgcgca
     7981 gtagcggcgg caccggcctg gaggtctgcg tctcagggtc ggcgaagaca tagatgagtt
     8041 cgcaggcgat cagcagttca tcgccgcgaa agatggcgct ggtgaagttc atcgacgagt
     8101 tgccgatgcg gccgcacttg agcgccacgt ccaggcgatc gtccatgcgc gccgacgcat
     8161 ggaattccac cgtggccttc ttgacgtagg tgtcgccgcc gagcatgtgc atcgcctcct
     8221 cgtacggcag cgccagcgca cgccagtagc ctgccaccgc cgtgtccaca tacatcaggt
     8281 aatgggcgtt gaacacgatc ttctgcatgt cgatttcggc ccagcgaact tgcaagcgat
     8341 gaaaaaagcg aaagtcttgt cgtttcatgg tttgagtgcc agtgccaggg agcgcgccgc
     8401 gtcggcatgg gcctgccggg cttcggggat cgcgcgcccc atctaaccgg ggtgttttca
     8461 ttctggcatg ggtgccgtta aagtgaggct ttcaatcgta catcccggga ccgccatggc
     8521 cttcatctac tacgtgaccc agatccagtt cgacttcggc gccgtcaacc tcctcaagca
     8581 ggaatgcgaa cgggtcggca tcaagcggcc gctggtcgtc accgaccccg gcgtcaaggc
     8641 cgccggcgtc ttgcgcaaag ccctggacgc gctgaccggg attccggtcg cggtattcga
     8701 ccagacgccc tccaatccga cggcagccgc ggtgcgcgcc gcggtggagg tgtacaaagc
     8761 caatggctgc gacgggctcg tcgcggtggg cggcggctcg gccatcgact gcgccaaggg
     8821 cgtggcgatc gcggccaccc atgaaggccc gctgacccgt tacgccacca tcgagggcgg
     8881 ctcgccgcgc atcaccgaca aggtcgcgcc gctgatcgcc gtgccgacca ccagcgggac
     8941 cggcagcgaa gtggctcgcg gagcgatcat catcgtcgac gatcaccgca agctggggtt
     9001 ccactcctgg cacctggtcc ccaagaccgc catctgcgat ccggagttga cgctgggcct
     9061 gccgccgaaa ctgacggccg ccacgggtat ggacgcgatc gcccattgca tggagacgtt
     9121 catgtcggcc gccgtcaatc cgccggccga cggcatcgcc ctggacggcc tggagcgcgg
     9181 ctgggccaac atcgagcggg cgacccgcga cggcagtgac cgcgaggcgc ggttcaactt
     9241 gatgagcgcc tccatggagg gcgcgatggc gttccagaaa ggcctgggct gcgtccactc
     9301 gctcagccac agcctgggcg gcgtggaccc acggcttcac cacggcacgc tcaacgccgt
     9361 gttcctgccc gcggtggtgc gcttcaatgc gcaggccgag tcggtccgaa aggataggcg
     9421 gctcgagcgc atggcccacg ccatggatct gcggtcggcc gcggacattc cggacgcgat
     9481 cagggacatg aacgcgcgcc tgggtttgcc ggacggactg gcggccatgg gagtgcggcg
     9541 cgaccagttc gaggaggtca tcaagggcgc gctggccgac cattgccaca agaccaatcc
     9601 acgcctcgcc tcctcgcgcg attacgagga catgctggcc gcatcgatgt aaggcgcagc
     9661 tgcgctgccc cggcacgacc tattttctcg ggccctgcag cttggcgaag gcgccggcga
     9721 tggcggtgtt cagcggctcg ccacgaggcg cgcgctgccc tcgtcccgcg ccttcgtaac
     9781 ggttgtcacg cgggccatcg cggcgggccg gggcttcgcc cagcttcatc gacagcgcga
     9841 tgcgcttgcg agccacgtcg acttccatca ccttcaccgt gacgatgtcg cccgtcttca
     9901 cgacctcgcg cgcatccttg acgaacttgt gcgacagctg gctgacgtgc accaggccgt
     9961 cctggtgcac acccaggtcc acgaacgcgc cgaaggccgc gacgttgctc accgtgcctt
    10021 ccagcatcat gccttcgcgc aggtcgccga tgccttccac gccctcgttg aaccgggcca
    10081 ccttgaaatc ggggcgcgga tcgcggccgg gtttttccag ctcgcccagg atgtccttga
    10141 cggtgatcac gccgaacttc tcgttggcga acagctcggg cttcagggtc ctgagcattt
    10201 ccgcgcggcc catcagctgc gccaccggct tgccggtcgc agccagcatt ctttcgacga
    10261 ccgggtaggt ctccgggtgg acgccggtca tgtccagcgg attctcgccg ccgcggatgc
    10321 gcaaaaagcc cgcgctttgc tcgaaggtcc tggcgcccag gccgctcact tccatcagct
    10381 gctggcggtt gctgaacgcg ccatgggttt cgcgccagcg cactaccgcc ttggccacgc
    10441 cggctgaaag gcccgacaca cgggccaaca gcggcacgct cgccgtattc aggtccacgc
    10501 ccaccgaatt gacgcagtct tccaccacgg cctccagcgt gcgggccagc tcgctctggt
    10561 tcacgtcgtg ctggtactgg cccacgccga tggatttggg atcgatcttc accagttcgg
    10621 ccagcggatc ctgcaggcgg cgggcgatgc tggcggcgcc gcgcaggctc acgtccacat
    10681 cgggcatttc ctgcgatgcg tattcgctgg ccgaatagac cgaggcaccg gcctcgctca
    10741 ccacgacgcg ctgaaggccg ccaggttcgg tatccaggcg cttgatcagg tcggcggcga
    10801 gcttgtcggt ttcgcggctg gccgtgccgt tgccgatggc gatcaggttc acgccgtgct
    10861 tgcggcacag cagctccaac tggtgcagcg acccatccca gtcgcggcgc ggctcgtgcg
    10921 gatagacggt cgcggtggca acgagtttgc cggtggcgtc taccacggcg accttgacgc
    10981 cggtgcggat gccggggtcc aggcccatca cgacacgcgg tccggccggt gccgccagca
    11041 gcaggtcgcg caggttgtcg gcgaagacct tgatcgccac cttctccgct tcctcgcgca
    11101 ggcgggcgaa caggtcgcgc tcggtggaaa gcgagagctt cacgcgccag gtccaggcca
    11161 cgcatttgcg cagcagatcg tcggcggcgc ggccctgatg gctccagccc aggtgcagcg
    11221 cgatcctgcc ttcggcgatc gagggccggc ccggctcggg ctccaccggc aaggcgagct
    11281 tggcctcgag aatctccagt tgccggccgc ggaacactgc cagcgcccgg tgcgaaggga
    11341 cgcgtccgat cggctcgtca tagccgaagt agtcgcgaaa cttcgccacg tcggcgttgt
    11401 tctcgtcctt gccggccatg agcttcgctt tgaacagtcc ttccgaccac agccattcgc
    11461 gcagttgccg caccagtgcc gggtcctcgg cccagcgctc ggaaaggatg tcgcgcaccc
    11521 catccagcac ggccgccacc gtcgtgaaat cttcgccgcc ctctcctttt ccggcgtgga
    11581 cgaagggcag cgcctccggg gcgggatcga gcgacggatc ggcgaagagc ctgtccgcca
    11641 acggctcgat ccccgcttcg cgcgcgatct gccccttggt gcgccgcttc ggtttgtacg
    11701 gcaggtacag atcctccagc tcctgcttgg tcggcgcggc ctcgatggcc gcgcgcagga
    11761 cgggcgtgag cttgccttgc tcctcgatgc ttttcaggac ggcgctgcgc cgcccttcga
    11821 gttcgcgcag atagcccagc cggtaatcca gttcgcgcag ctggatatcg tccagcccgc
    11881 cggtcgcttc cttgcggtaa cgcgcaacga acggcaccgt ggcgccgccg tcgaacaagt
    11941 caaccgccgc ctggacctgg tgttcgtgga cccggatttc cgcggcgatg cgccggatga
    12001 tcttttgcat tctgtaactg ctattgctgg agagcttcga ggacgcggca gtttgccaca
    12061 gagggcggac aggacctcgg catctcggct gacaccgcgg cggcaggcac gccgaccaaa
    12121 atggcttatt cctctccaag gagactcacg atgcgtgccc gactgttttt tcttgtcctt
    12181 gcgatcttgc tggtagccgg atttgccgcc cagaactggt ccgaattcat gcgtacttcg
    12241 cccctcacct tcggtgtggt ggaggagcag gcggcgctgg ggctgatcat gctcggcgca
    12301 ctgggtgtcg cgctgatggc gttcctcctc agcagcgcga tgcaggagtc gcggatgtgg
    12361 ctcgaatcga gccgacacgc caagagcctg caagcgcagc gcgatctggc ggagaaggcc
    12421 gaggcgtccc gcttcaccga actgcgcca
//
LOCUS       DASWIY010000444         5904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2186862, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000444 DASWIY010000000
VERSION     DASWIY010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186862"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1454)
                     /locus_tag="VGA59_15785"
     CDS             complement(225..1454)
                     /locus_tag="VGA59_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9721179.1"
                     /translation="MLTLDFHPTGRHFLQIPGPSPVPDRILRAMSLPTIDHRGPEFGA
                     LGLRVLAGLAKIFQTRHPVIIYPASGTGAWEAALANVTSPGDQVLMYETGHFATLWNK
                     MATRLGLKTEFLGLPGTEGWRRGVQADMIEERLKADTRHAIKAVCVVHNETSTGVTSN
                     ILAVRKAIDAARHPALLMVDSISGLACADYRHDEWGVDVTISGSQKGLMLPPGISFNA
                     LSPRAIEASKGAKLPRSFWAWDEMIETNKSGYFPYTPNTNLLYGLAEACDMLLAPEHG
                     GLPAVFARHERWGEGVRAAVKAWGLEIQCADSAVYSPVLTGVMMPEGVDADAVRRLIY
                     ERFDCSLGTGLGKVKGRMFRIGHLGDCNDLTLVAALAGCEMGLKLAGVKLAASGVQAA
                     MEFFASHPAPMLLRKAA"
     gene            1569..2264
                     /locus_tag="VGA59_15790"
     CDS             1569..2264
                     /locus_tag="VGA59_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9721180.1"
                     /translation="MTADVISIPRAALHEQVARRLRQMLVEGGIPPGAKLNERELTEI
                     LQVSRTPLREAIKMLAAEGLVELLPNRGAIAVALDETDVRHTFEVMAGLEAMSGELAA
                     QRITDAELAEIKAMQFEMMAAYTRRDLSNYYRLNALIHSAINAAAGNPVLTATYNQVN
                     ARLQALRFRSNQDGEKWRRAVKEHETMIEALAAHDPAAMREVLLSHLFNKRDVVLEQL
                     RREADAQPSGVAQ"
     gene            2261..5344
                     /locus_tag="VGA59_15795"
     CDS             2261..5344
                     /locus_tag="VGA59_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9721181.1"
                     /translation="MNAPLPLKVARQVAGQTYDTVAGAANEVAGRLAQRLAAETQGEV
                     LFSRADRGRYATDASIYQVMPVGVFVPHSSEDVKLALDICRDLKVPIVPRGGGTSQCG
                     QTVGAGLIVDYSKHVRKILHFDAATRTAEVEPGMVLDHLNLALKKLGLWYPVDVSTSA
                     QATLGGMAGNNSCGSRSIAYGNMVHNVLGAQAWTADGELHRFGRFDESIGTARAIGQR
                     IKELAAALAPEIDEHWPKVLRRVGGYNLDIFRNQNERPYTGNDSVNLAHLLVGSEGTL
                     ALTRSLTLQLSELPKAKVLGVVNFPTFYKAMDSAQHIVKLGNASASAGSADRTLTAVE
                     LVDRTMIELSLQNPAFAPVIRTAVIGRPDAVLLVEFSGDDKAALVRKLDELVELMGDL
                     GLPGSVVRMEDDAPQKNLWEVRKAGLNIMMSLKGDGKPVSFIEDCAVPLEHLAEYTDA
                     LTQVFRRHGTRGTWYAHASVGTLHVRPILDMRAGGAPKMRAIAEEASALVRKYKGAYS
                     GEHGDGLCRGEWIAWQFGPKINEAFARIKAIFDPLNLLCPQRVIDPPRMDDTRLMRFS
                     PGYKVIALRTALNWRAWDVQNDPATERVTPPGTGGDPAQGFAKAVEMCNNNGHCRKFD
                     AGTMCPSYRVTRDEKHSTRGRANTLRLALSGQLGPEGLAGDEVRQALELCVSCKGCKR
                     DCPTGVDMARMKVEFLHHYKKKHGYTLKDRIVARLPDYAHWASRFAGLLNQRNRSSLL
                     AALGEKLLGFSARRSLPAWQSRHFFNSAPVTATAQQVLAAARPVVLFVDTFNGFFESG
                     NARAALQLLQAAGYTVHVATKWLADGKHLCCGRTYLAGGMVDEARAKAREVVEALLPF
                     TDKAIPIVGLEPSCLLTLRDEMLAMDLGAPAQAIAGHALLLEEFLARESQAGRLEQLK
                     KKLRPLDKRVLVHGHCHQKAFAAVAPIVEVLRLIPGVQPEVIESSCCGMAGSFGYEAG
                     HYDVSMQMAELSLLPAVRKYPGAIVVADGTSCRHQIKDGAGREAVHAALLLAAQL"
     gene            5354..5803
                     /locus_tag="VGA59_15800"
     CDS             5354..5803
                     /locus_tag="VGA59_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9721182.1"
                     /translation="MDAPSIRSALPSDEAAWRELWHGYCDFYGAKLGEDVTDRTWKRI
                     LDPDSAIMCVVAEVDGQVYGFANCVVHENTWETQPVCYLEDLFVLPSARGHGIGKALI
                     EWLRNAMRAEGWARLYWVTRQDNEPARKLYDQFAQSDGFVRYVIRQR"
BASE COUNT         1036 a         1982 c         1950 g          936 t
ORIGIN      
        1 caagagtggg aaagcgattt tcttcatgtc atgtctcctt tagtgatcaa acttgcctgt
       61 catcccggac ttgtctttct gtcatcccgg actcgatccg ggatccacca tcttgggatg
      121 gattgcaggt caagcccgca atgacagccc tttgcgggtc gagcccgcaa tgacagccct
      181 tttctcaatt tgcgagccca gcccgcaatg acagcctttt ctttctaggc tgccttgcgc
      241 aacaacatcg gtgccgggtg cgaagcgaag aactccatcg ccgcctgcac gcccgaggcc
      301 gccagtttca cgcccgcgag cttcagcccc atctcgcagc cggccagcgc cgccaccagc
      361 gtcaggtcgt tgcagtcgcc caggtggccg atgcggaaca tcctgccctt gaccttgccc
      421 aggcccgtgc ccagggagca gtcgaagcgc tcgtagatga gcctgcgcac ggcgtccgca
      481 tcgacgccct cgggcatcat cacgccggtg agcaccggcg aatagaccgc ggagtcggcg
      541 cactggattt ccaggcccca ggccttgacg gccgcacgca cgccctcgcc ccagcgctca
      601 tggcgcgcga acaccgccgg caagccgccg tgttcgggcg ccagcagcat gtcgcaggcc
      661 tcggccagcc cgtacaacag gttggtattg ggtgtgtagg ggaagtatcc gctcttgttc
      721 gtttcgatca tctcgtccca ggcccagaac gacctgggca gcttcgcgcc cttgctcgcc
      781 tcgatcgccc tgggcgaaag cgcgttgaag ctgatgcccg ggggcagcat caggcctttt
      841 tgcgaaccgc tgatggtgac gtccacgccc cattcgtcat gcctgtagtc ggcgcaagcc
      901 agccccgaga tgctgtccac catcaacagc gccggatgcc tggcggcatc gatggccttg
      961 cgcaccgcga ggatgttgct cgtgacgccg gtggaggtct cgttgtgcac cacgcacacg
     1021 gccttgatgg cgtgccgggt gtcggccttc agccgctctt cgatcatgtc ggcctgcacg
     1081 ccgcgccgcc agccttcggt gcccggcagg cccaggaatt cggtcttcag gcccagccgc
     1141 gtggccatct tgttccacag cgtggcgaag tggccggtct cgtacatgag cacctggtcg
     1201 ccgggactcg tcacattggc cagggccgcc tcccatgcgc cggtgccgga ggccggatag
     1261 atgatgaccg gatgccgggt ctggaaaatc ttcgccaggc ccgcgagcac ccgcaggccg
     1321 agtgcgccga actccgggcc gcgatgatcg atcgtgggca ggctcatggc gcgcaggatg
     1381 cggtcgggaa ccggcgacgg gcccgggatc tgcaggaaat gccgccccgt ggggtggaag
     1441 tcgagcgtca gcatcacgat gtccttggaa aaagctccga tgatttttgc atgcaaaact
     1501 caaatgtcaa tggcgactct actgatcacg gactttacat ccttgttttt tgcatacaaa
     1561 attgcgacat gaccgcagac gtgatctcga tcccccgcgc cgccttgcat gagcaggtcg
     1621 cccggcgcct gcgccagatg ctggtggaag gcggcattcc gcccggcgcc aaactcaacg
     1681 aacgcgagtt gaccgaaatc ctgcaggtgt cgcgcacgcc gctgcgcgag gccatcaaga
     1741 tgctggccgc ggaggggctg gtcgagctgc tgcccaatcg cggcgccatc gccgtggcgc
     1801 tggacgagac cgacgtgcgc cataccttcg aagtgatggc cggcctggaa gccatgtccg
     1861 gcgaactcgc ggcccagcgc atcaccgacg cggaactggc cgagatcaag gccatgcagt
     1921 tcgagatgat ggctgcctac acgcggcgcg atctgtccaa ctactaccgg ctcaatgcgc
     1981 tgatccacag cgccatcaac gccgcggccg gaaaccccgt gttgacggcc acctacaacc
     2041 aggtcaatgc ccggctgcag gcgctgcgct tccgctcgaa ccaggatggc gagaaatgga
     2101 gacgcgcggt gaaggagcac gaaacgatga tcgaagcgct ggccgcgcac gatcccgcgg
     2161 caatgcgcga ggtcttgctg agccacctgt tcaacaagcg cgatgtcgtg ctcgagcaat
     2221 tgcgccgcga ggccgatgcc cagccttccg gggtggcgca atgaacgcgc cgctgccttt
     2281 gaaggtcgcg cggcaggtgg ccgggcaaac ctacgacacc gtcgccggcg ccgccaacga
     2341 agtggccggc cggctcgcgc agcggctggc cgccgagacg cagggcgagg tgctgttctc
     2401 cagggccgac cgcggccgtt atgccaccga tgcctcgatc taccaggtca tgcccgtggg
     2461 cgtgttcgtg ccgcacagca gcgaggacgt caagctggcc ctggacatct gccgcgacct
     2521 gaaggtgccg atcgtgccgc gcggcggcgg caccagccag tgcgggcaga cggtgggcgc
     2581 cgggctgatc gtcgattact ccaagcatgt gcgcaagatc ctgcacttcg acgcggccac
     2641 gcgaaccgcc gaggtggagc cgggcatggt gctggaccac ctgaacctgg cgctgaagaa
     2701 gctgggcctg tggtatccgg tggatgtgtc caccagcgcg caggccacgc tgggcgggat
     2761 ggccggcaac aactcctgcg gcagccgcag catcgcctac ggcaacatgg tccacaacgt
     2821 gctgggcgct caggcctgga cggcggacgg cgagttgcac cggttcggcc gctttgacga
     2881 gtccatcggc accgcgcgag ccatcggaca gcggatcaag gaactcgcgg ccgcgctggc
     2941 ccccgagatc gacgagcact ggcccaaggt gctgcggcgc gtgggcggct acaacctcga
     3001 catcttccgc aaccagaacg agcggcccta caccggcaac gactcggtca acctggcgca
     3061 cctgctggtc ggcagcgaag gcaccctggc cctgacgcgc tcgctcacgc tgcagctgag
     3121 cgaactgccg aaagccaagg tgctcggggt ggtgaacttc ccgaccttct acaaggcgat
     3181 ggactcggcc cagcacatcg tgaagctggg gaacgcaagc gcaagcgcag ggtccgcgga
     3241 ccgcacgctg acggccgtgg aactggtgga ccgcacgatg atcgagctgt cgcttcagaa
     3301 cccggccttc gcgccggtga tccgcaccgc cgtgatcggc cggcccgatg cggtgctgct
     3361 ggtggagttt tccggcgacg acaaggcggc cctggtgcgc aagctcgacg agctggtgga
     3421 gttgatgggc gacctgggcc tgcccggttc cgtggtgcgc atggaggacg acgcgccgca
     3481 gaagaacctg tgggaagtgc gcaaagccgg cctgaacatc atgatgagcc tgaaaggcga
     3541 cggcaagccg gtgagcttca tcgaggactg cgccgttccg ctggagcacc tggccgaata
     3601 cacggacgcg ctcacccagg tgttccgcag acatggcacc aggggcacct ggtatgcgca
     3661 cgcgtccgtc ggcaccctcc atgtgcggcc gatcctggac atgcgcgcag gcggcgcgcc
     3721 caagatgcgc gccatcgccg aagaagcctc ggccctggtg cgcaagtaca agggcgccta
     3781 cagcggcgag cacggcgacg gcctgtgccg gggcgaatgg atcgcgtggc agttcgggcc
     3841 gaagatcaac gaagcgttcg cccggatcaa ggcgatcttc gacccgctga acctgctgtg
     3901 cccccagcgc gtgatcgacc cgccgcgaat ggacgacacc cggctgatgc gtttttcacc
     3961 cggctacaag gtcattgcgc tgcgcacggc gctgaactgg agggcctggg acgtgcagaa
     4021 cgacccggcc accgaacgcg tcacgccgcc cggcacgggg ggcgatccgg cgcaaggctt
     4081 cgccaaggcg gtggagatgt gcaacaacaa cggccactgc cgcaagttcg acgccggcac
     4141 catgtgcccg agctaccggg tgacgcgcga cgagaagcac tcgacgcgcg ggcgggccaa
     4201 cacgctgcgg ctggcgctgt ccgggcagct cggccccgag gggctggccg gcgacgaggt
     4261 ccgccaggcc ctggagctgt gcgtgagctg caagggctgc aagcgcgatt gccccacggg
     4321 cgtggacatg gccaggatga aggtggagtt cctgcaccac tacaagaaga agcacggcta
     4381 cacgctcaag gacaggatcg tggcgcggct gcccgattac gcgcactggg cgagccggtt
     4441 cgccggcctg ctgaatcagc gcaaccgcag ctctttgctc gcggcgctgg gcgagaaact
     4501 gctgggcttc tcggcgcggc gcagcctgcc ggcctggcag tcgcgccact tcttcaacag
     4561 cgcaccggtc acggcgacgg cgcaacaggt gttggccgcc gcgcggcccg tggtcctctt
     4621 cgtggacacg ttcaacggct ttttcgaatc cggcaatgcg cgggccgcgc tccagctgct
     4681 gcaggccgcc ggctacacgg tccacgtggc gaccaagtgg ctggccgacg gcaagcacct
     4741 gtgctgcgga cgcacctacc tggccggcgg catggtcgat gaagccaggg ccaaggcccg
     4801 cgaagtcgtg gaggccttgc tgccgttcac cgacaaggca attcccatcg tcggcctgga
     4861 gccttcctgc ctgctgacct tgcgcgacga aatgctggcg atggacctgg gagcgccggc
     4921 ccaggcgatc gccgggcatg cgctgctgct ggaggagttc ctggcacgcg agtcccaggc
     4981 cgggcgcctg gagcaattga agaagaagct gcgcccgttg gacaagcgcg tgctggtgca
     5041 cggccactgc caccagaagg ccttcgccgc ggtggcgccg atcgtcgagg tgctcaggct
     5101 gattcccggc gtgcagcccg aggtgatcga aagcagctgc tgcggcatgg ccggcagctt
     5161 cggctacgag gccggtcatt acgatgtctc gatgcagatg gccgagctga gcctgttgcc
     5221 ggcggtgcgc aagtaccccg gcgccatcgt cgtcgccgac ggcaccagct gccgccacca
     5281 gataaaggac ggggccggcc gggaagcggt gcacgcggcg ctgttgctgg cggctcagct
     5341 ttaaactgcc gccatggacg caccgtcgat ccgctccgcc ctgccttccg acgaagccgc
     5401 gtggcgcgag ctgtggcacg gctattgcga tttctatggc gccaaactcg gcgaggacgt
     5461 caccgatcgc acgtggaagc gcatcctcga cccggactcg gccatcatgt gcgtcgtggc
     5521 cgaggtcgac gggcaggtct acggcttcgc caactgcgtg gtccatgaga acacctggga
     5581 aacccagccg gtgtgctacc tggaagacct gttcgtcctg ccttccgccc gcggccacgg
     5641 catcggcaag gccctgatcg aatggctgcg caacgcgatg cgcgccgaag gctgggcgcg
     5701 gctgtactgg gtgacgcgcc aggacaacga acccgcccgc aagctctacg accagttcgc
     5761 gcagtcggac gggttcgtcc gctacgtgat caggcagcgc taaccgcagg ccccccgcac
     5821 cctagcccct agcccctagc ccctagcccc tagcccctca acaatcatgt accgcctcca
     5881 ctgcttcagc caatcgggca acag
//
LOCUS       DASWIY010000445        21269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1816008, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000445 DASWIY010000000
VERSION     DASWIY010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 21269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21269
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816008"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VGA59_15805"
     CDS             complement(<1..444)
                     /locus_tag="VGA59_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX9721183.1"
                     /translation="MSEPNKEDELAGTEQPFVAHLVELRDRLIKASIAIGVVAAALFL
                     YPGPGALYDILAAPLVAHLPKGSTMIATSVISPFLVPLKILLMTAFLVALPVVLYQVW
                     AFVAPGLYSHEKKLVVPLVVSSTLLFFVGVGFCYFFVFGQVFSFIQ"
     gene            complement(460..918)
                     /gene="tatB"
                     /locus_tag="VGA59_15810"
     CDS             complement(460..918)
                     /gene="tatB"
                     /locus_tag="VGA59_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX9721184.1"
                     /translation="MIDLGIEKMLVIGAVALVFIGPEKLPRVARTVGTLLGKAQRYVA
                     DVKAEVNRSMELEELKKMKDTVETAARDVEGSIRSGASEFEKEWSEATTAAQSALSEA
                     PSFPEYRHPKKNWRIKQGAMPLWYKARNGIRTKALSGAARVARYRPQKFS"
     gene            complement(956..1195)
                     /gene="tatA"
                     /locus_tag="VGA59_15815"
     CDS             complement(956..1195)
                     /gene="tatA"
                     /locus_tag="VGA59_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEX9721185.1"
                     /translation="MGSFSIWHWLIVLLIIVLVFGTKKLKNIGSDLGGAVKGFKDGIK
                     EGSATETPVPPAGQVTSSQPGSEKTTIDVEARNKS"
     gene            complement(1353..1718)
                     /locus_tag="VGA59_15820"
     CDS             complement(1353..1718)
                     /locus_tag="VGA59_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX9721186.1"
                     /translation="MSAAADSTCIFCKIAEGKIPSRKVYEDEEIFVFHDIHPWAPVHF
                     LMVPKAHIASLAQVGPEHAALLGRMMTLAPKLALQEGCRPYPEGGYRIVINTGAEGGQ
                     EVQHLHIHVFGGPRPWLKG"
     gene            complement(1738..2094)
                     /locus_tag="VGA59_15825"
     CDS             complement(1738..2094)
                     /locus_tag="VGA59_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721187.1"
                     /translation="MSETDFAQFEVKTANDRTVMHVLYGLHTLAWLSMGMLAVIALIV
                     NYIKRADEHDAVYATHHNYMIATFWWTVLWLVVTGPLWLLLVLPGAVAYAIVGLWYLY
                     RCLRGWLRFTDGRPPF"
     gene            complement(2104..2472)
                     /locus_tag="VGA59_15830"
     CDS             complement(2104..2472)
                     /locus_tag="VGA59_15830"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX9721188.1"
                     /translation="MSSNDSLARLAAIIESRKQANGGNPDSSYVARLLHRGPDAFLKK
                     IGEEATEVVMAAKDAEQGGDRQKLVDEVADLWFHTMIALAHFGLEPADVIAELERREG
                     TSGIEEKAMRKARQREGAGE"
     gene            complement(2477..2869)
                     /gene="hisI"
                     /locus_tag="VGA59_15835"
     CDS             complement(2477..2869)
                     /gene="hisI"
                     /locus_tag="VGA59_15835"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX9721189.1"
                     /translation="MEWLDQVKWDGQGLVPVIAQEVGSNDVLMFAWMNREALRKTAEL
                     GRAVYFSRSRGKLWFKGEESGHVQAVHEIRMDCDNDVLLLKVEQLGHEPPIACHTGRH
                     SCFFHLYRNGAWEVAEPVLKDPGSIYKS"
     gene            complement(2930..3709)
                     /gene="hisF"
                     /locus_tag="VGA59_15840"
     CDS             complement(2930..3709)
                     /gene="hisF"
                     /locus_tag="VGA59_15840"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX9721190.1"
                     /translation="MLAKRIIPCLDVTGGRVVKGVNFVALRDAGDPVEIAARYNEQGA
                     DEITFLDITATSDDRDLILHIIEAVASQVFIPLTVGGGVRTVEDVRRLLNAGADKTSF
                     NSAAIANPQVISDASARYGAQCIVVAIDAKRRGPEQAATRGPGWDVYSHGGRRNTGLD
                     AVQWAVEMTRRGAGEILLTSMDRDGTRAGFDLELTRAVSDAVTVPVIASGGVGSLDHL
                     ADGIQQGGADAVLAASIFHYGEYTVAQAKARMAQRGIPVRL"
     gene            complement(3733..4476)
                     /gene="hisA"
                     /locus_tag="VGA59_15845"
     CDS             complement(3733..4476)
                     /gene="hisA"
                     /locus_tag="VGA59_15845"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX9721191.1"
                     /translation="MLLIPAIDLKDGHCVRLKQGDMDQVTTFGEDPAAMARGWLAKGA
                     RRLHLVDLNGAFAGKPQNFAAIKSILKAVGDDIPVQLGGGIRDLDTIEKYIDGGLRYV
                     IIGTAAVKNPGFLKDACTAFGGHIIVGLDAKDGKVATDGWSKLTGHEVIDLARKFEDW
                     GVESIVYTDIGRDGMLTGINIEATVKLAQALHIPVIASGGLSGIADIEKLCAVEDEGV
                     EGVICGRAIYAGDLDFVQAQAKADELNGR"
     gene            complement(4525..5169)
                     /gene="hisH"
                     /locus_tag="VGA59_15850"
     CDS             complement(4525..5169)
                     /gene="hisH"
                     /locus_tag="VGA59_15850"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX9721192.1"
                     /translation="MKTVAVVDYGMGNLRSVAQALMHVAAGTGFEVVVTARPQDVRAA
                     DRVVLPGQGAMPDCMRELRDSGLQAPVLEAAAGKPLFGVCVGMQMLLDRSQEGPTEGL
                     GLIHGEVLKFDLAGRLQPDGSRYKVPQMGWNQVWQTRHHPIWQGVPDGSYFYFVHSYY
                     ARPSDARHSAGEADYGTRFTAAIARDNIFATQFHPEKSADHGLALYRNFLQWNP"
     gene            complement(5225..5854)
                     /gene="hisB"
                     /locus_tag="VGA59_15855"
     CDS             complement(5225..5854)
                     /gene="hisB"
                     /locus_tag="VGA59_15855"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX9721193.1"
                     /translation="MTTPSRVPDTDPSNHRRAEVVRHTAETRITVKVDLDGSGQSRLA
                     TGIGFFDHMLDQIARHGLIDIEIQAEGDLHIDGHHTVEDVGITLGQAVYKAVGDKRGI
                     RRYGHAYVPLDEALSRVVIDFSGRPGLVMNVPFKSGMIGTFDSQLTHEFFQGFANHAF
                     VTLHIDNLRGENAHHQCETVFKAFARALRVALELDPRSAGTVPSTKGSL"
     gene            complement(5851..6930)
                     /gene="hisC"
                     /locus_tag="VGA59_15860"
     CDS             complement(5851..6930)
                     /gene="hisC"
                     /locus_tag="VGA59_15860"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX9721194.1"
                     /translation="MNRNTAGVADRVFRQDVQGMHAYAIQPSAGLIKLDAMENPHRLP
                     ADLQASLGRRLGALALNRYPGERTNDLRAALASHARMPEGFDIMLGNGSDELISLIAL
                     ACDVPGASILAPVPGFVMYAMSAQLQGLKFTGVPLTADFELDEAAMLAAVAEQRPSVI
                     YLAYPNNPTAKLWDDAVIEKIIESAPGLVVMDEAYQPFSSKSYIDRITRHGHVLLMRT
                     LSKFGLAGVRIGYLMGPAALVAQIDKVRPPYNVSVLNCEAALFALEHADVFASQARDV
                     RQQRALVQRALGELPGVKAWPSDANMILARVPDARKAFEGMKSLGVLVKNVSKMHPLL
                     ANCLRLTVGTAEENDRMLAALKTSL"
     gene            complement(6927..8240)
                     /gene="hisD"
                     /locus_tag="VGA59_15865"
     CDS             complement(6927..8240)
                     /gene="hisD"
                     /locus_tag="VGA59_15865"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX9721195.1"
                     /translation="MIAKPARLSTAAAGFEAQFTARLQWSAETDAGIEQRVTEILADV
                     QQRGDAAVLAYTNRFDELDATSVTGLELTQAELKTAFESLPAAQRDALQAAASRVRSY
                     HEAQKKANGESWSYRDGDGSLLGQKVTPLDRVGIYVPGGKAAYPSSVLMNAIPAHVAG
                     VGEIVMVVPTPRGEKNALVLAAAYVAGVSRAFTIGGAQAIAALAFGTATVPRVDKITG
                     PGNAYVASAKRQVFGKVGIDMIAGPSEILVLADGSTPADWVAMDLFSQAEHDELAQSI
                     LLCPDAGYIDRVQAEIDRLLPEMPRARIIAKSLSDRGALIQTRSMEEACEISNRIAPE
                     HLEIASRDPHRWEPLLRHAGAIFLGAFTSESLGDYCAGPNHVLPTGSTARFSSPLGVY
                     DFQKRSSLIEVSEAGAQKLGAIAAELAYGEGLQAHARAAEMRLKR"
     gene            complement(8237..8902)
                     /gene="hisG"
                     /locus_tag="VGA59_15870"
     CDS             complement(8237..8902)
                     /gene="hisG"
                     /locus_tag="VGA59_15870"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX9721196.1"
                     /translation="MITLALSKGRIFDETLPLLKAAGIEVLEDPEKSRKLILATSQPQ
                     VRVVIVRATDVPTYVQYGGADLGVTGLDTLIEHGAHWGRGTGLYQPLDLRIARCRVSV
                     AVRADFDYASAVRQGARLKVATKYVNIAREFFAAKGVHVDLIKLYGSMELAPLTGLAD
                     AIVDLVSSGGTLKANNLVEVERIMEISSRLVVNQAALKLKQDAIRRVIDAFAAALARE
                     LTQ"
     gene            complement(8899..10164)
                     /gene="murA"
                     /locus_tag="VGA59_15875"
     CDS             complement(8899..10164)
                     /gene="murA"
                     /locus_tag="VGA59_15875"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX9721197.1"
                     /translation="MDKLLIRGGRQLHGEVRISGAKNAALPELCAALLTAQPVVLENV
                     PRLQDVATMLTLIRNMGVAIDRDENGRVRIDSSGLSCPEAPYELVKTMRASVLALGPL
                     LARFGEATVSLPGGCAIGSRPVDQHIKGLQAMGAEIVVEHGYMIAKLPQGRERLTGAS
                     IRTDMVTVTGTENFLMAATLAQGETVLENAAQEPEIADLAEMLIKMGARIEGHGTSRI
                     TINGVEKLGGCTHQVVADRIEAGTFLCAVAATGGDVVLRHGRADHLEAVIDKLRDAGA
                     SVAALEDGIHLHSQGRLAPQSFRTTEYPGFPTDMQAQFMVLNAIADGPSKITETIFEN
                     RYMHVNELVRLGARVHVDGKFAVIDGVEKLSGATVMATDLRASASLVIAGLVAEGETL
                     VDRIYHLDRGYDRMEAKLRAIGADIERIK"
     gene            complement(10174..10419)
                     /locus_tag="VGA59_15880"
     CDS             complement(10174..10419)
                     /locus_tag="VGA59_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEX9721198.1"
                     /translation="MTSTEIETLISAGLACEYIQVEGDGRHWQATIVSPEFEGKSRIQ
                     RHQRVYRTLGAKMLTDEVHALSMHTVTPAEWAALNED"
     gene            complement(10468..11220)
                     /locus_tag="VGA59_15885"
     CDS             complement(10468..11220)
                     /locus_tag="VGA59_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9721199.1"
                     /translation="MTGWQTLFYKEILRFWKVSFQTVAAPVLTAVLYMLIFGHVLQDH
                     VKVYDISYTSFLVPGLVMMSVLQNAFANSSSSLVQSKIMGSLVFVLLTPLSHWSWFFA
                     YVGSSMVRGLVVGLGVFLITALFGEPSFVAPQWIVLFAVLGAAMLGALGLIAGLWAEK
                     FDQMAVFQNFVIMPMTFLSGVFYSIRSLPPFWQDMSHLNPFFYMIDGFRYGFFGVSDV
                     SPWLSLGIVASALLVVSAIAVHLLRIGYKIRG"
     gene            complement(11217..12221)
                     /locus_tag="VGA59_15890"
     CDS             complement(11217..12221)
                     /locus_tag="VGA59_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9721200.1"
                     /translation="MPAITFQSVDKIYPSARNGPLKALDGVSFDIQEGEFFGLLGPNG
                     AGKTTLISVLAGLVRPSAGRVVVRGHDVQSDYANARRQLGVVPQELVFDPFFNVRESL
                     RIQSGYFGVRNNDAWIDELLESLGLADKADANMRQLSGGMKRRMLVALALVHKPPVIV
                     LDEPTAGVDVELRQTLWQFIDRLNKAGHTVLLTTHYLEEAEALCGRIAMLKSGRVVAL
                     ERTSELLKAASSNVLRFKIDRPLPPELAGKARVTGRIVQLPAHDAHEIERYLAIIRQA
                     GLTVEDVEVRKADLEDVFLDVMSKHGEKPDAGAPREGARPPSGGTALDEEATLEAGSS
                     "
     gene            complement(12279..12551)
                     /locus_tag="VGA59_15895"
     CDS             complement(12279..12551)
                     /locus_tag="VGA59_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9721201.1"
                     /translation="MLILPAALTHEQAAACCRMLAQGMRSQADPAVVADVAALSRFDS
                     SALAVLLECRREALALGKTFSVARLPARLRELATLYGVAELLPGPP"
     gene            complement(12586..13227)
                     /locus_tag="VGA59_15900"
     CDS             complement(12586..13227)
                     /locus_tag="VGA59_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9721202.1"
                     /translation="MNRRVMAAMAAWLLALVAFAGSASPALAADEAPDAMIKRLSDEV
                     LALIRADKAIQAGDTQRILALVDSKIMPNVDFRRMTAAAVGPAWRRATPEQQKRLQDE
                     FKLLLVRTYSGALAQVDDQTISIKPQRSAPGDVDVVVRTEIRGRGDPIQLDYRLEKTP
                     GPGPGWKIYNLNVLGVWLVETYRSQFAQEINAKGLDGLIAALTERNKSNAKKG"
     gene            complement(13325..14152)
                     /locus_tag="VGA59_15905"
     CDS             complement(13325..14152)
                     /locus_tag="VGA59_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEX9721203.1"
                     /translation="MTKAFLFPAAGAIPRRALLALVAVAMLALGGCAGFNKNPRDPLE
                     PLNRKVMQFNEGVDAMVLKPVATVYMHGVPALARTGVSNFFGNLGDAWSFVNSALQFK
                     FQNAAENFMRLNVNTFFGLGGILDIASELNIERHKEDFGQTLGHWGIPAGPYIVLPLL
                     GPSTLRDTIALPLDRRADPLQRVNPAVDRNSLYVLRAVDARANLLRVGTLIEEAALDK
                     YSFTREAHLQRRRAAIIETKESDGSVPREGEPAPKPPGDAARPAPDGSRAPGPAPTR"
     gene            complement(14149..14634)
                     /gene="mlaD"
                     /locus_tag="VGA59_15910"
     CDS             complement(14149..14634)
                     /gene="mlaD"
                     /locus_tag="VGA59_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX9721204.1"
                     /translation="MERSKSDIWVGLFVLIGAASMLFLALQSANLLSLNLQASYRVVA
                     KFDNIGGLKRQAAVKSAGVVVGRVESIGFDDKSFQARVTLAMDSRYAFPKDSSLKILT
                     SGLLGEQYIGVEAGADENVLASGDTITATQSAVVLENLIGQFLYNKAADASTPATPGK
                     K"
     gene            complement(14685..15467)
                     /gene="mlaE"
                     /locus_tag="VGA59_15915"
     CDS             complement(14685..15467)
                     /gene="mlaE"
                     /locus_tag="VGA59_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEX9721205.1"
                     /translation="MTWLNPADVGFATRRKLADLGHGARLFMRLLSLVGASFRRFGLI
                     RDQVHFLGNYSLAIIAVSGLFVGFVLGLQGYYTLQRYGSSEALGLVVALSLVRELGPV
                     VTALLFAGRAGTSLTAEIGLMKAGEQLSAMEMMAVDPVRRVLAPRFWAGVIAMPLLAA
                     VFSAVGIIGGWVVGVVLIGVDPGAFWGQMQGGVDVWRDVGNGVVKSFVFGVTVTFIAL
                     LEGFEAQPTPEGVSRATTRTVVVASLSVLGLDFMLTAMMFSI"
     gene            complement(15464..16282)
                     /locus_tag="VGA59_15920"
     CDS             complement(15464..16282)
                     /locus_tag="VGA59_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9721206.1"
                     /translation="MTDPDSTALVECRHLRFGYGQRPILDDLSFTVERGKVTAVMGAS
                     GGGKTTVLRLIGGQIKAQQGELLFDGQEIAALDQPGLYAARRRMGMLFQFGALFTDLS
                     VFDNVAFPLREHTRLPESLVRDIVLMKLNAVGLRGARDLMPSEISGGMARRVALARAI
                     ALDPELIMYDEPFAGLDPISLGTAAQLIRRLNDTLGITSIVVSHDLEETFSIADQVIV
                     LANGRVAAQGTPQEVMQSSDPLVDQFVHARPDGPVHFHYPGPTVAQDFGGGFPG"
     gene            complement(16360..17826)
                     /locus_tag="VGA59_15925"
     CDS             complement(16360..17826)
                     /locus_tag="VGA59_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX9721207.1"
                     /translation="MGKITGFMEYERVEEGYPPVTERVKHHKEFVVGLTDEQAKIQGA
                     RCMDCGIPFCNSGCPVNNIIPDFNDLVYRTDWENAFAVLDSTNNFPEFTGRICPAPCE
                     EACVLNVNDEPVGIKSIERAIVDRAWQHGWVRPRPAKIRTGRKVAVVGSGPAGMAAAQ
                     QLARAGHDVTLFEKNDRIGGLLRYGIPDFKMEKTHIDRRVEQMKAEGVTFRTGVMVGN
                     LPEGSKVVNWAKETVAPKELLNQFDAVLLTGGAEQSRDLPVPGRDLGGIHFAMEFLPQ
                     QNKVNAGDKVKGQIRADGKHVVVIGGGDTGSDCVGTSNRHGALSVTQFELLPQPPEEE
                     NKPLTWPYWPYKLRTSSSHEEGCGREFAIATKEFIGERGRVTGLKTVRVEWKDGKMIE
                     IAGSEQVLKADLVLLAMGFVSPVSTILEGFGVEKDARGNAKATTDFIGGYATGVPKVF
                     AAGDMRRGQSLVVWAIREGRQAARAVDEFLMGFSDLPR"
     gene            complement(17881..>21269)
                     /locus_tag="VGA59_15930"
     CDS             complement(17881..>21269)
                     /locus_tag="VGA59_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX9721208.1"
                     /translation="WVERNKIELSQLPEPLTVYQPHLETLTIRQQVFGYTQEDIKFLM
                     SPMAQAGEEGIGSMGNDSPLAVLSSKDKPLYNYFKQLFAQVTNPPIDPIREAIVMSLV
                     SFIGPKPNLLDINQVNPPMRLEVSQPILDFADMAKLRDIESHTHGKFRCYTLDITYPL
                     AWGHEGVEAKLASLNAEAVDAIKGGKNILIISDRAVGPTQVAIPAVLALSSIHQHLVR
                     EGLRTTSGLVVETGTAREVHHFGVLAGYGAEAVHPYLAMETLISVHKDLPGDLSPDKA
                     IYNYVKAVGKGLSKIMSKMGVSTYMSYCGAQLFEAIGLNSDTVNKYFTGTASRVEGIG
                     VFEIAQEAIRTHKAAFGDDPVLANMLDAGGEYAWRTRGEEHMWTPDAIAKLQHATRAN
                     NWNSYKEYAQLVNDQSRRHMTLRGLFEFRIDPTRAISIDDVEPAKEIVRRFATGAMSL
                     GSISTEAHTTLAVAMNRIGGKSNTGEGGEDPARYRQELKGIPIAQGQTIAEIIGRDVI
                     EVDYPLKEGDSLRSKIKQIASGRFGVTAEYLGSADQIQIKMAQGAKPGEGGQLPGGKV
                     SKYIGFLRHSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVAPHATVSVKLVSEVGVG
                     TVAAGVAKCKSDHVVIAGHDGGTGASPWSSIKHAGSPWEIGLAETQQTLVLNRLRGRI
                     RVQADGQMKTGRDVVIAALLGADECGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPV
                     LRRKFAGKPEHVVNYFFFVAEEVRQIMAQLGVRKFDDLIGRTDLLDIKKGIEHWKAKG
                     LDFSRLFARPNVPAEVPRFHQEEQDHGLEKSLDRILIERSRPAIEKGEKVQFIEIARN
                     VHRSVGAMLSGAITKVHPEGLPDNTIHIQLEGTGGQSFGAFLTRGVTLYLIGDANDYT
                     GKGLSGGRIVVRPSIDFRGAATDNIIIGNTALYGATAGDAFFSGVAGERFAVRLSGAT
                     AVVEGTGDHGCEYMTGGTVLVLGKTGRNFAAGMSGGIAYVYDEDGQFASRCNTAMVSL
                     ERVGLATEQKVASKTRLHKDQADETLLHKLLEEHNRWTGSRRARELLDHWTVARTKFV
                     KVFPNEYKRALAEMYEREVEAASTGNNAHVAMKHESVAAA"
BASE COUNT         3505 a         7066 c         6849 g         3849 t
ORIGIN      
        1 ctggatgaac gagaacacct gcccgaacac gaagaagtaa cagaacccca ccccgacgaa
       61 aaacagcagg gtgctggaca cgaccagggg cacaaccagc tttttttcgt gcgagtacag
      121 ccctggagcg acgaaagccc agacctggta cagcaccacc ggcagggcca ccaggaaagc
      181 cgtcatcagc aggattttca gcggcaccag aaagggcgaa atcaccgatg tggcgatcat
      241 cgtggagccc ttgggcaggt gcgccaccag cggcgcggcc aggatgtcgt acagcgcccc
      301 cgggcccggg tacaggaaca gcgccgccgc caccacgccg atggcgatcg aggccttgat
      361 cagccggtcg cgcagctcca ccaggtgggc gacgaagggc tgctcggtgc cggcgagctc
      421 gtcttctttg ttgggttcgg acattagggt aaacaggccc taactgaatt tttgcgggcg
      481 ataacgggcg acccgggccg ctccggaaag cgctttggtg cggatcccgt tgcgcgcctt
      541 gtaccagagc ggcatggcgc cctgcttgat gcgccagttc ttcttcgggt gcctgtactc
      601 gggaaaggat ggcgcctcgc tgagcgccga ttgggccgcg gtggtggctt ccgaccactc
      661 tttctcgaac tcgctcgcgc cggaacggat ggaaccctcg acgtcgcgcg ccgcggtctc
      721 caccgtatcc ttcatcttct tgagctcttc cagctccatc gagcgattga cctcggcctt
      781 cacgtcagcc acgtagcgct gggccttgcc caggagcgtg cccacggtcc tggccacgcg
      841 cggcagcttt tcagggccga tgaaaaccag cgccacggcg ccgatcacca gcattttttc
      901 gatgccgagg tcgatcacga ttttcttccg gcagccgccc cgccgctttg cgatttcagg
      961 acttgttcct ggcttccacg tcgatcgtgg tcttctcgga tccgggctgc gaggatgtga
     1021 cctggcccgc cggcggcacc ggcgtttcgg tcgcggagcc ctccttgatg ccatccttga
     1081 agcccttgac ggcaccgccc aggtcggagc cgatgttctt gagcttcttg gtgccgaaga
     1141 ccagcacgat gatcaacagc acgatcagcc agtgccagat ggaaaatgaa cccatggtta
     1201 tgcctcctgc cgagccgccc ggggcccggc atgcgccccc gcggggggca gtgaatggat
     1261 gtgaatgccg gagtacatgt tttttactcg tgtacagggg tggattctag tcgggcaggg
     1321 gaagttcagc ccctgccaag gcctttgcgc gttcagcctt tgagccacgg tcgcggcccg
     1381 ccgaacacgt ggatgtgcag gtgctggacc tcctgcccgc cctcggcgcc ggtattgatc
     1441 acgatgcggt agcctccctc gggatagggg cggcagcctt cctgcagcgc cagcttgggc
     1501 gccagcgtca tcatgcggcc cagaagggct gcatgttcgg gccccacctg cgccagcgag
     1561 gcgatgtgcg ccttgggcac catcaggaag tgcacgggcg cccagggatg gatatcgtgg
     1621 aagacgaaga tttcctcgtc ttcgtacacc ttgcgcgagg gaatcttgcc ctcggcgatc
     1681 ttgcagaaaa tgcaggttga atcggcagca gcgctcatag gcttttgatg gaaaggatca
     1741 gaacggtggg cggccgtcgg tgaagcgtaa ccagccgcgc aggcagcggt acaggtacca
     1801 cagcccgacg atggcgtacg cgacggcacc cggaaggacg agcagcagcc acagcggccc
     1861 ggtgaccacc agccacagga cggtccacca gaacgtggcg atcatgtaat tgtgatgggt
     1921 cgcgtagacc gcgtcgtgct cgtcggcgcg cttgatgtag ttcacgatca gcgcgatcac
     1981 ggccagcatt cccatgctca accaggccag ggtatgcagg ccgtacagca catgcatgac
     2041 ggtacggtcg ttcgcggtct tgacctcgaa ctgggcaaaa tcggtttcag acatggcgcc
     2101 gcctcactct cccgcgcctt cgcgctgacg cgccttgcgc atggccttct cctcgatgcc
     2161 gctggtgccc tcgcggcgct ccagttcggc gatcacgtcc gccggctcca gcccgaaatg
     2221 ggccagggcg atcatggtgt ggaaccacag gtcggccacc tcatcgacca gtttttgccg
     2281 gtcgccgccc tgctcggcat ccttggccgc catgaccacc tcggtcgcct cctcgccgat
     2341 cttcttgagg aaggcgtccg ggccgcggtg cagcagccgc gccacatagc tcgaatccgg
     2401 atttccgcca ttggcctgct tgcggctttc gatgatggcc gccaggcggg ccagggagtc
     2461 gttcgagctc atggcgtcat gacttgtaga tggacccggg gtctttcaag accggctcgg
     2521 ccacttccca ggcgccattg cggtaaagat ggaaaaaaca gctgtgacgg ccggtgtggc
     2581 aggcgatcgg aggctcgtgc cccagctgct cgactttcag cagcagcacg tcgttgtcgc
     2641 aatccatgcg gatctcgtgc acggcctgca cgtgcccgga ctcctcgccc ttgaaccaga
     2701 gcttgccgcg cgagcggctg aaataaacgg cgcgcccgag ttcggcggtt ttccgcaatg
     2761 cctcccggtt catccaggcg aacatcagca cgtcgttgct gccgacttcc tgggcaatca
     2821 cggggaccag gccctggcca tcccacttga cttgatcgag ccattccatg gcccttattg
     2881 tgacggatgg cgggcccgag ccggccggtc gtgcacccgc gaggctggat cacagccgca
     2941 cgggaatgcc gcgctgggcc atgcgggcct tggcctgggc cacggtgtac tcgccgtaat
     3001 ggaagatgct cgcggccagc accgcatcgg cgccaccctg ctggatgccg tcggcgaggt
     3061 ggtccaggct gcccacgccg ccggaagcga tgaccggcac cgtgaccgcg tcgctcacgg
     3121 cgcgggtgag ctcgaggtcg aatccggccc tggtaccgtc gcggtccatg ctggtcagca
     3181 ggatctcgcc ggcgccccgg cgcgtcatct cgacggccca ttgcaccgcg tccaggccgg
     3241 tgttcctgcg gccgccatgg ctgtagacat cccagcccgg ccctcgggtg gcggcttgtt
     3301 ccggcccgcg gcgcttggcg tcgatggcca ccacgatgca ctgggcaccg tagcgggccg
     3361 aagcgtcgga aatcacctgc gggttcgcga tggcggccga gttgaagctg gtcttgtcgg
     3421 cacccgcgtt gagcagccgc cgcacgtcct ccacggttct cacgccgccg cccaccgtca
     3481 gcgggatgaa aacctgcgag gccacggctt cgatgatgtg caggatcagg tcgcggtcgt
     3541 cgctggtggc ggtgatgtcc aggaaggtga tttcgtcggc gccctgctcg ttgtaacgcg
     3601 ccgcgatctc caccggatcg ccggcatcgc gcagcgccac gaagttgacg cctttgacga
     3661 cgcgcccgcc ggtcacgtcc aggcagggga tgatgcgttt ggcaagcata ttggggagga
     3721 gttcagcaac cctcaacgcc cattgagttc gtcggccttg gcctgggcct ggacgaaatc
     3781 gagatcgccc gcatagatcg cgcggccgca gatcacgccc tcgaccccct cgtcctcgac
     3841 ggcgcagagt ttttcgatgt ccgcaatgcc cgacagccca ccggaggcaa tcaccgggat
     3901 atgcagggcc tgggccagct tgacggtggc ttcgatgttg atgcccgtga gcatcccgtc
     3961 gcgcccgatg tcggtgtaga cgatggattc gacgccccag tcctcgaact tgcgggccag
     4021 gtcgatgacc tcatggccgg tcagcttgct ccagccgtcg gtggcgacct tgccgtcctt
     4081 cgcgtcgagg ccgacgatga tgtggccgcc gaaagccgtg cacgcatcct tgaggaagcc
     4141 cgggttcttg accgcggcgg tgccgatgat gacgtagcgc aggccgccgt cgatgtattt
     4201 ctcgatggtg tccaggtcgc ggatgccgcc tcccagctgc accgggatgt cgtctcccac
     4261 cgccttgagg atggacttga tggcggcgaa attctggggc ttgccggcga aggccccgtt
     4321 caggtccacc aggtgcagcc gccgcgcgcc cttggccagc cagccgcgcg ccatggccgc
     4381 cgggtcctcg ccaaaggtcg tcacctggtc catgtcgcct tgcttgaggc gcacacagtg
     4441 gccgtctttc aggtcaatcg cgggaatgag gagcataacg gacgaaaacg gggcaacccg
     4501 acaggcgggc cgcctcgtcc tgagtcaagg attccattgg aggaagtttc ggtagagagc
     4561 caggccgtgg tctgcgcttt tttcggggtg gaactgggtc gcgaaaatat tatcgcgtgc
     4621 aatcgcggcg gtaaagcgcg tgccgtaatc ggcttcgccg gcgctgtggc gtgcatccga
     4681 cggccgcgcg tagtagctgt gaacgaaata gaagtagctg ccatcgggca cgccctgcca
     4741 gatcgggtgg tgccgggtct gccagacctg gttccagccc atttgcggca ccttgtagcg
     4801 gcttccgtcc ggttgcagcc gccccgccag gtcgaatttg aggacctcgc cgtggatcag
     4861 gcccaggcct tcggtgggac cttcctggct gcgatcgagc agcatctgca tgcccacgca
     4921 caccccgaac agcggcttgc cggccgcggc ctccagcacc ggcgcctgga gccccgagtc
     4981 gcgcaattcc cgcatgcagt cgggcatcgc gccctgcccg ggcaacacca cgcggtcggc
     5041 ggcgcgcacg tcctgcgggc gcgccgtgac gacgacctcg aagccggtgc ctgcggccac
     5101 gtgcatgagc gcctgcgcca ccgagcgcag gttccccatg ccatagtcga cgactgccac
     5161 ggttttcata gccagctgaa tacccgaaac gaaggcctcg agaccggaac ggcgccagga
     5221 gtaatcagag cgagcctttg gtcgagggaa cggttcctgc cgaacgcgga tcgagctcga
     5281 gcgccacccg cagggcgcgc gcgaaggcct tgaataccgt ttcgcattga tggtgcgcat
     5341 tttcgccgcg caaattgtct atgtgcagcg tgacgaaggc gtgattcgcg aatccctgga
     5401 agaactcgtg ggtcagctgg ctgtcgaaag tgccgatcat gccgctcttg aacggcacgt
     5461 tcatgaccag ccccggacgg cccgaaaaat cgatcacgac gcgcgacagc gcctcgtcca
     5521 gcggcacata ggcgtggccg tagcgccgga tcccgcgctt gtcgcccacg gccttgtaca
     5581 ccgcctgccc cagcgtgatg cccacgtctt cgaccgtgtg gtggccgtcg atgtgcaggt
     5641 cgccctcggc ctggatctcg atgtcgatca ggccgtggcg ggcgatctgg tcgagcatgt
     5701 ggtcgaaaaa accgatcccg gttgcgagcc tggactggcc gctgccgtcg aggtcgacct
     5761 tcaccgtgat ccgcgtttcc gccgtgtggc gtaccacctc cgcgcgccgg tggttgctcg
     5821 gatctgtgtc ggggacgcgc gaaggggtgg tcataaggaa gttttgaggg cggccagcat
     5881 gcggtcgttc tcttcggccg ttccgaccgt gaggcgcagg caattggcca gcaatgggtg
     5941 cattttagaa acgttcttga ccagcacgcc cagggatttc atcccctcga aggcctttcg
     6001 cgcatcgggc acgcgcgcca ggatcatgtt ggcgtcgctc ggccaggcct tgacgccggg
     6061 cagctcgccc aatgcgcgct ggacgagggc ccgctgctgt cgcacgtccc gggcctggct
     6121 ggcgaatacg tcggcatgct cgagggcgaa caacgccgct tcacagttga gcacgctgac
     6181 gttgtagggc gggcgtacct tgtcgatctg ggcaaccagg gccgccgggc ccatcaggta
     6241 tccgatgcgc acgcccgcca gcccgaactt gctcagggtg cgcatcagca gcacgtggcc
     6301 atgccgggtg atccggtcga tgtagctctt gctggaaaag ggctggtagg cctcgtccat
     6361 gaccaccagg ccgggcgcgg actcgatgat cttctcgatg acggcgtcgt cccacagctt
     6421 ggcggtcggg ttgttgggat atgccagata gatcaccgac ggccgctgct cggccaccgc
     6481 ggccagcatc gccgcctcgt ccagctcgaa atcggctgtc agcggcacgc cggtgaactt
     6541 cagcccctgc agctgggcgc tcatggcgta catcacgaaa ccgggcacgg gcgcaaggat
     6601 ggacgcgccc ggcacgtcgc aggcgagagc gatcagcgag atcagctcgt cggacccgtt
     6661 gcccagcatg atgtcgaaac cctccggcat ccgcgcatgg cttgccagcg cggcacgcaa
     6721 atcgttcgtg cgttcgcccg gatagcggtt cagagccagc gcaccgagcc gccggcccag
     6781 actggcctgc aggtcggcgg gcaagcggtg cgggttttcc atcgcgtcga gcttgatcag
     6841 gccggccgag ggttggatcg cgtaggcgtg catgccctgc acgtcctggc gaaacacacg
     6901 gtccgccacg ccggcggtat tcctgttcat cgtttgagcc tcatctcggc ggcgcgagcg
     6961 tgcgcctgca ggccttcgcc gtaagccagt tcggcggcga tcgcgcccag tttctgcgca
     7021 cccgcttcgc tcacctcgat caggctgctg cgcttctgga agtcatacac gcccagcggg
     7081 ctggaaaaac gcgccgtgct gccagtcggc agcacgtgat tggggccggc gcagtaatcg
     7141 cccagcgatt cgctggtgaa agcgcccaga aagatggcgc ccgcatgcct gagcagaggc
     7201 tcccagcggt ggggatcccg gctggcgatc tccaggtgtt ccggcgcgat ccggttgctg
     7261 atctcgcagg cctcctccat gctgcgggtc tggatcagcg cgccacgatc gctgagcgat
     7321 ttcgcgatga tccgggcccg cggcatttcg ggcagcaggc ggtcgatctc ggcctgcacc
     7381 cggtctatgt atccggcgtc gggacacagc aggatgcttt gcgcgagttc gtcgtgctcg
     7441 gcctgggaga acaggtccat ggccacccag tccgccggtg tgctgccgtc cgccagcacc
     7501 aggatctcgc tcgggccggc gatcatgtcg atgccgactt tgccgaacac ctgccgcttg
     7561 gcactggcca catacgcgtt tcccgggccg gtgatcttgt ccacgcgcgg cacggtcgcg
     7621 gttccgaaag ccagcgccgc gatggcctgc gcgccgccga tggtgaaggc ccggctcacc
     7681 ccggccacgt aagcggcggc aaggaccaag gcgttctttt cgccgcgcgg cgtgggcacc
     7741 accatgacga tctcgccgac tccggcgacg tgggccggaa tggcattcat cagaaccgac
     7801 gagggatagg ctgccttgcc tcccgggacg tagatgccga cccggtccag cggcgtcact
     7861 ttctggccca gcaggctgcc gtcgccgtcg cggtaactcc agctctcgcc attggccttc
     7921 ttctgcgctt catggtagct gcgcacacgg gacgcggcgg cttgcagggc gtcgcgctgc
     7981 gcagccggca ggctttcgaa agccgttttc aactcggctt gcgtgagctc caggccggtc
     8041 accgaggttg catcgagctc gtcgaagcga ttggtatagg ccagcacggc ggcgtccccg
     8101 cgctgctgca cgtccgccag gatttcggtg acgcgctgct cgatcccggc gtccgtttcg
     8161 gccgaccact gcaagcgtgc cgtgaactgc gcctcgaaac cggcggcggc ggtcgacagc
     8221 cgggcgggct tggcgatcat tgcgtcaatt ccctggcgag cgcggccgcg aaggcgtcga
     8281 tgacgcggcg aatggcatcc tgcttgagct tgagtgccgc ctggttgacc accagccgcg
     8341 agctgatctc catgatgcgc tcgacctcga ccaggttgtt ggccttgagc gtgccgccgc
     8401 tggagaccag gtccacgatc gcgtcggcca ggccggtgag cggcgcgagc tccatgctgc
     8461 cgtacagctt gatcaggtcc acgtgcacgc ccttggcggc gaaaaactca cgcgcgatgt
     8521 tcacgtattt cgtggcgacc ttcaggcgcg cgccctgtct caccgcgctg gcgtaatcga
     8581 agtcggcacg caccgccacg ctcacgcggc acctggcgat ccgcaggtcc agcggctggt
     8641 acaagccggt gccgcggccc cagtgggcgc cgtgttcgat cagcgtgtcc aggccggtga
     8701 cgccgagatc ggccccgccg tactgcacat aggtgggaac gtccgtcgcg cgaacgatca
     8761 ccacgcgaac ctgtggctgg ctggtggcca ggatcagctt gcgcgatttc tcgggatcct
     8821 ccagcacctc gatcccggcg gccttcagca gcggcagggt ctcgtcgaag atgcgtccct
     8881 tggacagggc cagtgtgatc atttgatccg ttctatatcc gcgccgatcg cgcgcagctt
     8941 ggcttccatg cggtcgtagc cgcgatccag gtgatagatg cggtccacca gggtttcgcc
     9001 ttcggccacg agcccggcga tgacgaggct ggcggaagcc cgcaggtcgg ttgccatcac
     9061 ggtggcgccc gacagctttt ccaccccgtc gatcaccgcg aacttgccgt ccacgtggac
     9121 cctcgcgccc aggcgcacca gctcgttgac gtgcatgtag cggttctcga agatcgtctc
     9181 ggtgatcttg gacgggccgt cggcgatcgc gttgagcacc atgaactggg cctgcatgtc
     9241 ggtcgggaag ccggggtatt cggtcgtgcg gaagctctgg ggcgcgagcc gcccctggga
     9301 gtgcaggtgg atgccatcct cgagggcggc cacgctggcg ccggcgtcgc gcagcttgtc
     9361 gatcacggct tcgaggtggt cggcccgccc atgccgcagc acgacgtcgc cgccggtcgc
     9421 ggcaaccgcg cacaggaagg tgccggcctc gatccggtcg gccaccacct ggtgcgtgca
     9481 gccgcccagc ttctccacgc cgttgatcgt gatgcggctg gtgccgtgcc cttcgatgcg
     9541 agcccccatc ttgatcagca tctcggccag atccgcgatt tccggctcct gggccgcgtt
     9601 ctccagcacg gtttcgcctt gggcgagcgt cgcggccatc aggaagttct cggtgcccgt
     9661 cactgtcacc atgtcggtgc ggatgctggc tcccgtcagc cgctcgcgcc cctgcggcag
     9721 cttggcgatc atgtacccgt gttcgaccac gatctcggcg cccatggcct gcaggccctt
     9781 gatgtgctgg tccaccggcc gcgatccgat ggcgcagccg ccgggcagcg aaacggtggc
     9841 ttcgccgaag cgcgccagca gggggcccag cgcgagtacc gacgcgcgca tggtcttcac
     9901 cagctcgtaa ggagcctcgg gacagctgag cccgctcgaa tcgattcgca cccggccgtt
     9961 ctcgtcgcgg tcgatggcca cgcccatgtt gcggatcagc gtgagcatgg tggcgacgtc
    10021 ctgcaggcgc gggacgttct ccagcacgac cggctgcgcc gtcagaagcg cggcgcacag
    10081 ctcgggcagc gccgcgttct tggcgccgga tatccggacc tcgccgtgga gctggcggcc
    10141 cccgcgaatc aagagcttgt ccatgaatgt gcttcagtct tcgttcagtg ccgcccactc
    10201 ggccggcgtc accgtgtgca tcgagagcgc atgcacctcg tcggtcagca ttttcgcacc
    10261 cagtgtcctg tagacgcgct ggtggcgctg gatgcggctc ttgccttcga attcgggcga
    10321 aacgattgtg gcttgccaat ggcggccatc gccttcgacc tggatgtact cgcaggccag
    10381 gcccgcactg ataagggttt caatttcggt agaggtcatg ggcaaagccg cggcacggcc
    10441 gcggaagtgg gcggttgttg gtaattccta tccccggatt ttgtatccga tgcgcagcag
    10501 gtgcacggcg atggcgctca ccaccagcag ggccgaggcg acgatgccca ggctgagcca
    10561 gggcgacacg tcgctgacgc cgaagaatcc gtagcgaaag ccgtcgatca tgtagaaaaa
    10621 cgggttcagg tggctcatgt cctgccagaa cgggggcagc gaccggatgg aatagaacac
    10681 gcccgacagg aacgtcatgg gcatgatgac gaagttctgg aacaccgcca tctggtcgaa
    10741 tttctcggcc cacaagccgg cgatcaggcc cagggcgccg agcatggcgg ctcccagcac
    10801 cgcgaacagc acgatccact gcggcgccac gaagctcggc tcgccgaaca acgcggtaat
    10861 caggaaaaca ccgaggccga cgaccagccc ccgcaccatc gatgagccga cgtaggcgaa
    10921 gaaccagctc cagtgggaaa gcggagtcag gagaacgaat accaggctgc ccatgatctt
    10981 gctctggacc agcgaggagg agctgttggc gaatgcgttc tgcagcacgc tcatcatcac
    11041 cagcccaggc accaggaaag acgtgtaact gatgtcgtag accttgacgt ggtcctgcag
    11101 cacgtggccg aagatcagca tgtagaggac cgccgtgagc accggcgccg ccacggtctg
    11161 gaagctcacc ttccagaagc gcaggatctc cttgtagaaa agtgtctgcc agcccgtcat
    11221 gaggaaccgg cctccagggt cgcctcttca tccagtgcgg tgccaccgga gggaggcctc
    11281 gcgccttccc gcggcgcgcc ggcgtccggc ttttcgccat gcttggacat gacatcgagg
    11341 aaaacgtctt ccaggtccgc cttgcggacc tccacgtctt ccaccgtcaa cccggcctgg
    11401 cggatgatcg ccagatagcg ttcgatctcg tgcgcgtcat gggccggcag ctgcacaatg
    11461 cgcccggtga cccgggcctt gccggcgagc tcggggggca gcggccggtc gatcttgaac
    11521 cgcaacacat tgctggacgc cgccttgagc agctcgctgg tgcgttccag cgccaccacc
    11581 cgcccggact tgagcatggc gatgcgcccg cacagggcct cggcctcttc caggtagtgg
    11641 gtggtcagca gcaccgtgtg gcccgccttg ttcaggcgat cgatgaattg ccacagcgtc
    11701 tggcgcagtt cgacatcgac gcccgccgtc ggctcgtcca gcacgatcac gggcggcttg
    11761 tgcaccagcg ccagcgccac cagcatccgc cgtttcatgc ccccggacag ctggcgcatg
    11821 ttggcgtcgg ccttgtccgc cagccccagg ctttccagca gttcgtcgat ccaggcatcg
    11881 ttgttcctca cgccgaaata acccgactgg atgcgaagcg actcgcgaac gttgaagaac
    11941 gggtcgaaca ccagttcctg cggcaccacg ccgagctgcc gccgcgcgtt cgcgtaatcg
    12001 gactgcacgt cgtggccgcg caccaccacc cggcccgcac tcggccgcac gagcccggcc
    12061 aggacgctga tcagcgtcgt cttgccggcg ccattggggc cgagcagccc gaagaactcg
    12121 ccctcctgga tgtcgaaact cacgccgtcc agggccttca atggaccgtt gcgggcggaa
    12181 ggatagatct tgtcaaccga ctggaaggtg atcgcgggca tgggaacacg cgattttacc
    12241 gaggggcggc cccgcggcgc ccgccgcgcc aaatacgcct acggcggccc cggcagcagt
    12301 tcggcgacgc cgtagagcgt ggcgagctca cgcagccgcg ccggcaggcg ggcgaccgag
    12361 aaagtcttgc ccagcgccag ggcctcgcgc cggcattcca gcagcacggc cagagccgac
    12421 gaatcgaacc tcgagagggc tgcgacgtcc gccaccacgg ccgggtcggc ctgcgatcgc
    12481 atcccctgcg ccagcatccg gcagcatgcc gccgcctgct cgtgagtgag tgctgccggc
    12541 aggatcagca tggccttgcc cgccctggaa cggcccgatc gcccatcatc ccttcttcgc
    12601 gttggacttg ttgcgctcgg tcaatgcggc gatcaggccg tccaggccct tcgcattgat
    12661 ttcctgggcg aactggctgc ggtaggtttc caccagccag acgccgagga cgttcaggtt
    12721 gtagattttc cagcccgggc ccggacccgg cgttttttcc aggcggtaat ccagctggat
    12781 cgggtctccg cggccgcgga tttcggtgcg taccaccaca tcgacatcac cgggggcgct
    12841 gcgctgcggc ttgatgctga tggtctggtc atcgacctgc gccagcgcgc ccgagtacgt
    12901 gcgaaccagc aacagcttga attcgtcctg gagccgcttt tgctgctcgg gcgtggcccg
    12961 gcgccaggcc gggcccacgg cagccgccgt catgcgccgg aaatccacgt tgggcatgat
    13021 cttgctgtcc accagcgcca ggatccgttg ggtgtcgccg gcctgaatgg ccttgtccgc
    13081 cctgatcagg gccaacactt cgtcggacag ccgcttgatc atggcgtccg gcgcttcgtc
    13141 ggccgccagc gccggcgacg ccgagcccgc gaacgcaacc agagccagca gccaagcggc
    13201 cattgcggcc ataacacgtc tattcattca atttcctttc atgcggaaac ctccgcggtc
    13261 aaaccttgga atgccggtgc ctggcccggt tccggcgtcc caccaccgga tacagcttga
    13321 aaccctagcg cgtgggggcc ggccccggcg cgcgcgaacc gtccggcgcg ggtcgggctg
    13381 catcacccgg cggtttaggc gcgggttcgc cctccctggg cacggagcca tccgactcct
    13441 tggtctcgat gattgccgcc cggcgcctct gaaggtgcgc ctcccgcgtg aagctgtact
    13501 tgtccagcgc ggcctcttcg atcagggttc ccaccctcag caggttggcg cgcgcgtcca
    13561 cggcccgcag cacgtagagc gaattacggt ccaccgccgg gttgacccgc tgcagcgggt
    13621 cggcccggcg atcgagcggc aacgcgatcg tatcgcgcaa ggtcgacggg cccagcagag
    13681 gcagcacgat gtacggcccg gctggaatgc cccaatggcc gagcgtctgg ccgaaatctt
    13741 ccttgtggcg ttcgatattc agctcgctgg cgatgtccag gatgccgccg aggccgaaaa
    13801 aggtgttgac gttcagacgc atgaaattct cggccgcgtt ctggaacttg aattgcaacg
    13861 cgctgttgac gaacgaccag gcgtcgccca ggttgccgaa gaaattgctg accccggtgc
    13921 gcgccagtgc cgggacgcca tgcatgtaga ccgtcgcgac cggcttgagc accatcgcat
    13981 cgacgccttc gttgaactgc atgaccttgc gattgagggg ctccagcgga tcacgcgggt
    14041 tcttgttgaa tcccgcgcaa cccccaagcg ccagcatggc caccgccacg agcgccagga
    14101 gcgccctgcg tggaatggcg cccgcggcgg gaaagaggaa tgctttcgtc attttttgcc
    14161 cggtgtcgcc ggggtactgg cgtcggccgc cttgttgtag aggaactggc cgatcaggtt
    14221 ttccagcacc acggctgact gagtagccgt aatggtatcg cctgacgcca gaacattctc
    14281 gtcggccccg gcttcgaccc cgatgtactg ctccccgagc aagccgctgg tgaggatttt
    14341 cagcgaactg tccttcggaa acgcgtagcg gctgtccatc gcgagcgtga cccgcgcctg
    14401 gaacgacttg tcgtcgaagc cgatcgattc caccctgccg accacgacgc cggcgctttt
    14461 cacggccgcc tggcgcttga gcccgcctat gttgtcgaac ttggccacca cccggtagct
    14521 ggcctgcagg ttcagcgaca gcaggttggc cgactgcagc gccagaaaaa gcatggacgc
    14581 cgccccgatc agcacgaaca ggcccaccca tatgtcgctc ttggaacgtt ccatcgcggt
    14641 ctttcggttg gggacagagc tgaagatctg tcccgcaaga tatatcagat actaaacatc
    14701 atcgccgtga gcatgaaatc caggcccagc accgagagcg aagcgaccac cacggtgcgc
    14761 gtagtcgccc gcgagacgcc ttcgggtgtg ggctgcgcct cgaagccctc cagcaaggcg
    14821 atgaaggtca ccgtcacgcc gaagacgaaa ctcttgacca cgccgttgcc gacgtcgcgc
    14881 cacacgtcga ccccgccttg catctggccc cagaatgcgc cggggtccac gccgatcagc
    14941 accacgccca ccacccagcc accgatgatg cccaccgcgc tgaagacggc cgccagcagc
    15001 ggcatggcaa tgaccccggc ccagaagcgc ggcgccagca cccggcgcac aggatcgacc
    15061 gccatcattt ccatggcgct cagttgctcg ccagccttca tcaatccgat ttcggcggtg
    15121 agcgaggtgc cggcacgccc ggcgaacaac aaggccgtga cgaccgggcc cagctcgcgc
    15181 accaggctca acgccacgac gaggcccagt gcttccgacg agccgtagcg ctgcaaggtg
    15241 taataaccct gcagtcccag cacaaaaccg acgaacaggc cggacaccgc gatgatggcc
    15301 agcgaatagt tacccaggaa atggacctgg tcgcgaatca ggccgaaacg ccggaaactt
    15361 gccccgacga gggacagcag ccgcatgaac aaccgggccc cgtggcccag atcggccagc
    15421 ttgcgccgtg tcgcgaagcc cacatcggcg ggattgagcc aggtcatccc gggaaccctc
    15481 ccccgaaatc ctgcgcgacg gtcgggccgg gatagtggaa gtgcacggga ccgtccggcc
    15541 tggcatgcac gaactggtcg accagcgggt cgctggattg catcacctct tgcggtgtgc
    15601 cctgtgccgc cacccggccg ttggccagca cgatcacctg gtcggcaatg ctgaaggttt
    15661 cctccaggtc gtgcgacacg acgatgctgg tgatacccag cgtgtcgttg agccggcgga
    15721 tcagctgggc cgccgtaccc agggagatcg gatcgagccc ggcgaacggc tcgtcgtaca
    15781 tgatcagttc cgggtccagg gcgatcgcgc gcgccagcgc cacgcgccgc gccatccccc
    15841 cggagatctc gctgggcatc aggtcgcgcg caccgcgaag gccgacggcg ttgagcttca
    15901 tcagcacgat gtcgcgaacc aggctttcgg gcaggcgcgt gtgctcgcgc agcgggaagg
    15961 cgacgttgtc gaacacgctc aggtccgtga acaaggcacc aaactggaac agcatgccca
    16021 tgcggcgacg ggcggcatag aggcccggct ggtccagcgc ggctatctcc tgcccgtcga
    16081 acagcagttc gccctgctgg gccttgatct gccccccgat cagccgcaac accgtcgtct
    16141 tgccgccgcc ggaagcgccc atgaccgcgg tgaccttgcc acgttccacc gtgaagctca
    16201 ggtcatcgag gatggggcgc tgcccgtagc caaacctcag gtggcggcat tcgaccagcg
    16261 ccgtgctatc gggatcggtc atccggctgg acaggccggg atcaagcgcg gcccttaatg
    16321 gaaagaccga tgataaaggc tggagctatt agctgggcct taacgcggca ggtcactgaa
    16381 ccccatcagg aattcgtcga ccgcgcgcgc ggcctgccgt ccttcccgga tggcccacac
    16441 gaccagggat tggccgcggc gcatgtcacc ggcggcgaac accttgggca cgccggtcgc
    16501 atagccgccg atgaagtccg tggtggcctt ggcattgccg cgagcgtcct tctcgacacc
    16561 aaaaccctcg agaatggtgg acacggggct gacgaaaccc atggccagca gcaccaggtc
    16621 ggccttgagg acctgttcgc tgccggcaat ctcgatcatc ttgccgtcct tccactcgac
    16681 ccggacggtc ttgagcccgg tgacccggcc tctttcgccg atgaattcct tcgtggcgat
    16741 ggcgaattcc cgcccgcagc cttcttcatg gctggagctg gtgcgcagct tgtagggcca
    16801 gtatggccag gtcagcggct tgttctcttc ctcgggcggc tgcggcagca gctcgaactg
    16861 cgtgacactg agcgcgccgt gacggttgct ggtgcccacg cagtcggaac ccgtgtcgcc
    16921 gccgccgatg acgaccacat gtttgccgtc ggcccgaatc tggcccttga ccttgtcgcc
    16981 ggcattcacc ttgttttgct gcggcaggaa ctccatggcg aaatggatgc cgccgaggtc
    17041 gcggccgggc acgggcaggt cgcgcgactg ctcggcgccc ccggtcagca gcacggcgtc
    17101 gaactgattc agcagttcct tcggcgcgac ggtttccttg gcccagttca ccaccttcga
    17161 gccttccggc agatttccca ccatcacccc ggtgcggaac gtcacgcctt cggccttcat
    17221 ctgctcgaca cgccggtcga tatgcgtctt ctccatcttg aagtcgggga tgccgtagcg
    17281 caacaggccg ccaatgcgat cgtttttctc gaacaaggtc acgtcgtggc cggcacgcgc
    17341 aagttgctgc gccgcggcca tcccggcggg acccgagccg accaccgcca ctttcctgcc
    17401 ggtgcggatc ttggccggcc gcggcctgac ccagccgtgc tgccaggcgc ggtcgacgat
    17461 cgcccgctcg atcgacttga tgccgaccgg ttcgtcattg acgttgagca cgcaggcctc
    17521 ttcgcacggc gccgggcaga tgcggccggt gaactccggg aaattgttgg tggagtccag
    17581 cacggcgaag gcgttctccc agtcggtccg gtacaccaga tcgttgaagt ccggaatgat
    17641 gttgttgacc ggacagccgc tgttgcagaa cggtatgccg cagtccatgc agcgcgcgcc
    17701 ctggatcttc gcctgctcgt cggtgaggcc cacgacgaat tccttgtggt gcttcacgcg
    17761 ctccgtcacc gggggatagc cctcctcgac acgctcgtat tccatgaagc cggtgatttt
    17821 tcccatgttc ttttccttta cgggacagat tgctctgtcc ccgacatttc atccttctga
    17881 ctatgccgcg gcgaccgatt cgtgcttcat ggccacatgc gcgttgttgc cggtgctggc
    17941 ggcttccacc tcgcgctcgt acatctcggc cagcgcccgc ttgtattcgt tcgggaacac
    18001 cttgacgaac ttcgtgcggg cgaccgtcca gtggtccagc agttcgcgcg cccgcctgct
    18061 gccggtccag cggttgtgct cctccagcag cttgtgcaag agcgtttcgt cggcctgatc
    18121 cttgtgcagc cgggtcttgc tggccacctt ttgttccgtc gcgagaccca cgcgttccag
    18181 tgacaccatg gccgtgttgc accggctggc gaactggccg tcttcgtcgt acacataggc
    18241 aatgccgccc gacatgccgg cggcgaaatt gcggcccgtc ttgcccagca ccagcacggt
    18301 gccgccggtc atgtactcgc agccgtggtc accggttcct tcgacgacgg cggtcgcgcc
    18361 cgacaggcga accgcgaagc gctcgccggc aacgccgctg aagaatgcat cgccggccgt
    18421 ggctccgtaa agcgccgtgt tgccgatgat gatgttgtcg gtggcggcgc cgcggaaatc
    18481 gatgctcggg cgcacgacga tacggccgcc cgacaggccc ttgcccgtgt agtcattggc
    18541 atcgccgatc aggtacaggg tgacaccccg ggtcaggaac gctccgaacg actggccgcc
    18601 cgtaccttcg agctggatgt ggatcgtgtt gtcgggcaag ccctcggggt ggaccttggt
    18661 gatggcgccg gacagcatcg cgccgaccga gcggtggacg ttgcgcgcga tctcgatgaa
    18721 ctggaccttc tcgcctttct cgatggcggg gcgcgagcgc tcgatcagga tgcggtccag
    18781 gctcttttcc aagccgtggt cctgctcctc ctggtggaag cgcggcactt cggcaggcac
    18841 gttgggccgc gcgaacaggc ggctgaaatc caggcccttg gccttccagt gctcgatgcc
    18901 cttcttgatg tccagcaggt cggtgcggcc gatcaggtcg tcgaacttgc gcacacccag
    18961 ctgcgccatg atctggcgca cttcctcggc gacaaagaag aagtagttga cgacgtgctc
    19021 cggtttgccg gcgaacttcc tgcgcagcac cgggtcctgc gtcgccacgc ccaccgggca
    19081 ggtattgaga tggcacttgc gcatcatgat gcagccttcg accaccagtg gcgccgtcgc
    19141 gaaaccgcac tcgtcggccc ccagcagcgc cgcgatcacg acgtcgcggc cggtcttcat
    19201 ctggccgtcg gcctgcaccc ggatgcgccc gcgcaggcgg ttcagcacca gggtctgctg
    19261 ggtttcggcc aggccgattt cccaggggct gccggcgtgc ttgatggatg accagggcga
    19321 agcgccggta ccgccgtcat ggccggcgat caccacgtgg tcgctcttgc atttggcgac
    19381 gccggcggca acggtgccca cgccgacctc cgacaccagc ttgacgctga cggtggcatg
    19441 cggcgcgacg ttcttcagat cgtggatcag ctgggccagg tcctcgatcg aatagatgtc
    19501 gtggtgcggt ggcggagaga tcaggccgac gcccggcacc gagtggcgca ggaagccaat
    19561 gtactttgag accttgccgc ccgggagctg ccccccctcg cccggcttgg cgccttgggc
    19621 catcttgatc tggatctggt cggccgagcc caggtactcg gccgtcacgc cgaaacggcc
    19681 ggacgcgatc tgcttgatct tcgagcgcag cgaatcgccc tccttgagcg ggtagtcgac
    19741 ctcgatcacg tcccggccga tgatctcggc gatggtttgc ccctgcgcga tcgggatgcc
    19801 cttgagttcc tggcgatagc gcgcggggtc ttcgccgcct tcgccggtat tgctcttgcc
    19861 gccgatgcgg ttcatcgcga cggccagcgt ggtatgcgct tcggtcgaaa tcgagcccag
    19921 cgacatggcg cccgtcgcga accgcctgac gatctccttg gccggctcga cgtcatcgat
    19981 cgaaatggca cgggtagggt cgatcctgaa ttcgaacagg ccgcgcagcg tcatgtggcg
    20041 acggctctgg tcgttgacca gctgcgcgta ttccttgtag gagttccagt tgttggcccg
    20101 ggtggcatgc tgcagcttgg cgatggcgtc gggcgtccac atgtgctcct cgccgcgggt
    20161 acgccacgcg tattcgccgc cggcgtccag catgttggcg agcacgggat cgtccccgaa
    20221 tgcggccttg tgcgtgcgga tcgcttcctg cgcgatttcg aacacgccga tgccctcgac
    20281 acggctggcg gtgcccgtga agtacttgtt gacggtgtcg ctgttcagtc cgatggcctc
    20341 gaacagctgg gcgccgcaat agctcatgta cgtgctcacg cccatcttgg acatgatctt
    20401 ggacaggccc ttgccgactg ccttgacgta gttgtagatc gccttgtccg gcgacaggtc
    20461 gcccggcagg tccttgtgga cggagatcag ggtctccatc gccaggtagg gatgcacggc
    20521 ttccgcgcca tagcccgcca gcacgccgaa gtgatgcact tcgcgcgcgg tgccggtttc
    20581 gaccaccagt ccggacgtcg tgcgcaggcc ttcgcgcacc aggtgctggt ggatcgagga
    20641 cagtgccagc acggccggaa tcgccacctg cgtgggaccg acggcacgat cgctgatgat
    20701 caggatgttc ttgccgccct tgatcgcgtc cacggcctcg gcattcagcg aagcgagctt
    20761 ggcctccacg ccctcgtggc cccaggccag cggataggtg atgtccaggg tatagcagcg
    20821 gaacttgccg tgcgtgtggc tctcgatgtc gcgcagtttc gccatgtcgg cgaaatcgag
    20881 gatcggctgg ctgacttcca gccgcatggg cggattgacc tggttgatgt ccagcaggtt
    20941 gggcttgggg ccgatgaagg aaaccagcga catcacgatc gcctcgcgga tcgggtcgat
    21001 cggcgggttg gtcacctgcg cgaacagctg cttgaagtag ttgtacagcg gcttgtcctt
    21061 gctcgaaagg acggccagcg ggctgtcgtt acccatcgag ccgatgcctt cctcgccggc
    21121 ttgcgccatg ggcgacatca ggaacttgat gtcttcctgc gtgtagccga agacctgctg
    21181 gcggatcgtg agcgtttcga gatgcggctg atagaccgtg agtggctcgg gcagctggct
    21241 cagttcgatc ttgttgcgct cgacccatt
//
LOCUS       DASWIY010000446         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1065911, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000446 DASWIY010000000
VERSION     DASWIY010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065911"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VGA59_15935"
     CDS             <1..401
                     /locus_tag="VGA59_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX9721209.1"
                     /translation="QFHGDESPADCAAAGRPYLRAVRMAPGVDLINFAARYPDARAIL
                     LDAHVEGYGGGGKVFDWSLIPRNVPRPVVLSGGLHAGNVIEGILQVRPWAVDVSSGVE
                     SAKGIKDAEAISRFCEAVREADARMAASVA"
     gene            418..1683
                     /gene="trpB"
                     /locus_tag="VGA59_15940"
     CDS             418..1683
                     /gene="trpB"
                     /locus_tag="VGA59_15940"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX9721210.1"
                     /translation="MLNYRQPDARGHFGPYGGTFVAETLIHALDELKLAYAEVQNDPA
                     FVAEFQSELAHFVGRPSPVYHAARLSRELGGAQIYLKREDLNHTGAHKVNNTIGQALL
                     ARRMGKPRVIAETGAGQHGVATATICARYGLECVVYMGSEDVKRQSPNVYRMHLLGAK
                     VVPVESGSKTLKDALNEALRDWVTNVENTFYIIGTVAGPHPYPMMVRDFQRVIGDECL
                     VQMPQMIGRQPDAVIACVGGGSNAMGIFYPYIEHAGTRLIGVEAAGQGMDSGKHAASL
                     SAGSPGVLHGNRTYLLQDADGQITETHSISAGLDYPGVGPEHSYLKDIGRAEYVGVTD
                     DEALAAFHRLCRTEGIIPALESSHAVAYAMKMAPTLRTDQHLLVNLSGRGDKDIGTVA
                     DLSGASFYCRPSCQGQSVKGADVSVKGPR"
BASE COUNT          296 a          570 c          563 g          280 t
ORIGIN      
        1 tgcagttcca cggcgacgaa tcccccgccg actgcgcggc ggcgggccgg ccctacttgc
       61 gcgctgtgcg catggcgccg ggggtcgatt tgataaactt cgcggcccgg tatcccgacg
      121 cccgggccat cctgctcgac gcccatgtcg aaggctatgg cggtggcgga aaggtttttg
      181 attggtcact cattcctcga aacgtgcccc gtccagtcgt tttgtctggt gggttgcatg
      241 ccggaaatgt gatcgaaggg attcttcagg tgcggccttg ggccgttgat gtgagttccg
      301 gcgtcgaaag cgccaagggc atcaaggatg ccgaggcgat cagccggttc tgcgaggcgg
      361 tgcgcgaggc cgatgcccgc atggccgcca gcgtggcctg aagaacggat ttctttcatg
      421 ttgaattacc ggcaacccga tgcacggggg catttcggcc cctacggcgg caccttcgtc
      481 gccgagaccc tgatccacgc gcttgacgag ctgaaactgg cctacgccga ggttcagaac
      541 gatccggcct tcgtggccga attccagtcc gagctggccc atttcgtcgg ccggcccagc
      601 cccgtgtacc acgcggcgcg actcagccgc gaattgggcg gcgcgcagat ctacctcaag
      661 cgcgaagacc tcaaccacac cggcgcgcac aaggtgaaca acaccatcgg ccaggcgctg
      721 ctcgcgcgcc gcatgggcaa gccgcgcgtg attgccgaaa ccggcgccgg ccagcacggc
      781 gtggccaccg ccaccatctg tgcgcgctac gggctggaat gcgtggtcta catgggcagt
      841 gaggacgtga agcgccagtc gcccaacgtc taccgcatgc acctgctcgg cgccaaggtc
      901 gtgccggtgg aaagcgggtc caagacgctg aaggatgccc tcaacgaggc gctgcgcgat
      961 tgggtcacca acgtggagaa caccttctac atcatcggca ccgtcgcggg cccgcacccc
     1021 tatccgatga tggtgcgcga ctttcagcgc gtgatcggcg acgagtgcct ggtgcagatg
     1081 ccgcagatga tcggccgcca gcccgatgcg gtgatcgcct gcgtgggcgg cggcagcaac
     1141 gccatgggca tcttctatcc ctacatcgag cacgcgggaa cgcggctgat cggcgtcgag
     1201 gccgcagggc agggcatgga cagcggcaag cacgcggcct cgctgtcggc agggtcgccg
     1261 ggcgtgctgc atggcaaccg cacctacctg ctgcaggacg ccgacggcca gatcaccgaa
     1321 acgcattcga tctcggcggg gctcgactac cccggcgtgg gcccggagca ttcctacctc
     1381 aaggacatcg ggcgcgccga atacgtcggc gtgaccgacg acgaagcgct cgcggcgttc
     1441 catcgcctgt gccgcaccga aggcatcatc cccgcgctgg agtcgagcca tgcggtggca
     1501 tacgccatga agatggcgcc gaccctgcgc accgaccagc acctgctggt caacctctcg
     1561 ggccgcggcg acaaggacat cggcaccgtg gccgatctgt cgggagcctc gttctattgc
     1621 cggccctcct gccagggcca aagcgtcaag ggggcggacg tgtccgtcaa agggccccga
     1681 tgagccgcat tgccgctacc ttttccgcg
//
LOCUS       DASWIY010000447         4622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3611382, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000447 DASWIY010000000
VERSION     DASWIY010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3611382"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VGA59_15945"
     CDS             <1..577
                     /locus_tag="VGA59_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HEX9721211.1"
                     /translation="VEETSGFQAGKHGMRLAQGLSAMTPGVARQPMKTDGAHRELGCT
                     SCHGAHRFETKTAAVESCLGCHNDRHSLAYKQSPHYAMWQREAAGLAPAGSGVSCASC
                     HMPRVKMPTADGARIVVQHNQNDTLRPNEKMIRATCMACHGLGFSIDALADTRLVTGN
                     FRGMPSRHVVSIDMAIARKEEKQRERALGSP"
     gene            625..1203
                     /locus_tag="VGA59_15950"
     CDS             625..1203
                     /locus_tag="VGA59_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEX9721212.1"
                     /translation="MKRRAMLQGKRLAIGAGVVAAAAVPAWAAGISPQDMADALHAVM
                     AADRTVYTRLIVNRLQNEEKLIKASEHWKDEKALVLPAQMFRFGAEEVAKTKARFSYS
                     LLSAWPVNKQNAPKTPVEKEGLDFVAKNPGKNFYKEETLGKKTYFTAVYADSAIAPAC
                     VTCHNGHKDSPRTDFKIGDVMGGVVLRIPIDK"
     gene            1316..1732
                     /locus_tag="VGA59_15955"
     CDS             1316..1732
                     /locus_tag="VGA59_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HEX9721213.1"
                     /translation="MTPQQITLVQTSWQKVVPIKEKAAELFYGRLFEMDPSLQPMFKG
                     DIVEQGRKLTGMINTVVVKLDQLGDIVPAVQDLGKRHVAYGVKDEHYGVVAGALLWTL
                     GAGLGDAFTDDVRQAWTAAYTILAGAMKDAAATVQA"
     gene            complement(2039..3829)
                     /locus_tag="VGA59_15960"
     CDS             complement(2039..3829)
                     /locus_tag="VGA59_15960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01184.1"
                     /note="This model describes the ATP binding subunits of
                     ATP-binding cassette (ABC) transporters for nitrate
                     transport, or for bicarbonate transport, in bacteria and
                     archaea; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter ATP-binding protein"
                     /protein_id="HEX9721214.1"
                     /translation="MTAYSLKVPPSTAFLEIAPAAATSVPHVQRDAERPASSPGGYCL
                     ELRNLGKSYGAGAHRTQVLKDINLQIRQGEFVAIVGFSGSGKTTLISLIAGLIAADEG
                     EILHNGAPIAGPGPDRGVVFQSYSLMPWMTVRDNVALAVDQVYKDAPKAQRAERVHKY
                     IDMVNLTPALEKRPAELSGGMRQRVAVARALAANPDILLLDEPLSALDALTRAKLQDE
                     IIRIWSQEKKTVILITNDVDEGIMMADRIIPLNPGPGATFGPEFPVTLARPRDRAGMN
                     HSEDFKRIRKSVTRYLMDAGAQNSAGDGGNVIHLPSVRPNTDSEHRQAAKPDVAPEQV
                     PVRAERYVEFSAVSKIYPSRKGPVKVVEQFDLKIRKGEFVSVIGHSGCGKSTVLSMIA
                     GLTDVSEGGIFLDGREVMTAGPDRGIVFQAPSLVPWLTAYQNVGLGVEKVFPHASKKE
                     RKDIIEHYLERVGLADAAHKKALELSNGMKQRVGIARAFALSPKLLLLDEPFGMLDSL
                     TRWDLQEVLMEVWARTKLTAIMVTHDVDEAILLADRVVMMTNGPRARVGKVLEVDLPR
                     PRTRKALLEHPDYYQLREQLLDFLNECDHA"
     gene            complement(3843..>4622)
                     /locus_tag="VGA59_15965"
     CDS             complement(3843..>4622)
                     /locus_tag="VGA59_15965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9721215.1"
                     /translation="SLIAEHYAERDKAAAFLERQKAQRAEALKSDPKADTTIRPWTGK
                     PTYFDQILTSLKTVFTGFLIATLVAVPVGILCGLSRTVQAALNPLIQIFRPVSPLAWL
                     PIVTLVVSAAYVTSDGAWFQKSFLVSAITVTLCSLWPTLINTALGVSSIDKDLINVSK
                     VLRLSWTTKVLKIVVPSSLKFIFTGLRLSLGVGWMVLIAAEMLAQNPGLGKFVWDEFQ
                     NGSSNSLGRIMVAVFTIGLVGFLLDRIMHTLQQLVSFGDRV"
BASE COUNT          843 a         1450 c         1540 g          789 t
ORIGIN      
        1 cgtggaggaa accagcggct tccaggccgg caagcacggc atgcggttgg cgcagggcct
       61 ttcggcgatg acaccgggcg tggcgcgcca gcccatgaag accgacggcg cgcaccggga
      121 gctcggctgc acttcctgcc atggcgcgca ccgtttcgag acgaagacgg cggccgtgga
      181 atcgtgcctg ggctgccaca acgacaggca cagcctggcg tacaagcagt ccccgcacta
      241 cgcgatgtgg cagcgggaag cggccgggct ggcgccggcc ggctccggcg tgtcgtgcgc
      301 cagttgccac atgccgcgcg tgaagatgcc cacggccgat ggcgcgcgca tcgtggtgca
      361 gcacaaccag aacgatacgt tgcggcccaa cgaaaaaatg atccgcgcca cgtgcatggc
      421 ctgccacggc ctcggctttt cgatcgacgc gctggccgac acccggctgg tcaccggcaa
      481 cttcaggggc atgccgtccc ggcacgtcgt gagcatcgac atggcaattg caaggaaaga
      541 agagaagcag cgcgagcgcg cattggggag cccgtagccc atgggaatac ctgtggggtt
      601 tttttaaccg tgaaggagtg gaacatgaag agaagagcaa tgctgcaggg aaagaggctg
      661 gcgatcggcg cgggggtggt ggccgcggcc gccgttcccg catgggcggc cggcatcagt
      721 ccccaggaca tggcggatgc gctgcatgcc gtgatggcgg cggaccggac ggtctacacg
      781 cgcctgatcg tcaaccgcct gcaaaacgag gaaaagttga tcaaggccag cgagcactgg
      841 aaagacgaga aggcgctggt cttgccggcg cagatgttcc gcttcggcgc cgaggaagtg
      901 gccaagacca aggccaggtt cagctactcg ctgctgtccg catggccggt gaacaagcag
      961 aacgcgccga aaacgcccgt ggaaaaggaa ggcctggact tcgtcgccaa gaatccgggc
     1021 aagaacttct acaaggaaga aaccctcggc aagaaaacgt atttcacggc cgtgtatgcg
     1081 gacagcgcaa tcgcaccggc gtgcgtcact tgccacaacg gccacaagga cagcccgcgc
     1141 accgatttca agatcggcga cgtgatgggc ggcgtggtgc tgcgcattcc gatcgacaag
     1201 taggccggtg agcgccgccc ggttcatcgc cctggctgcc gtggctttgc cgccgcggcc
     1261 ggcaccggca gcagtggatt acatgtttgc aatcgtcaag taaaggagca ttccaatgac
     1321 accccaacaa atcaccctgg ttcaaacctc ctggcagaaa gtggtgccga tcaaggagaa
     1381 ggccgccgaa ctgttctacg gcaggctgtt cgagatggac ccgtcgctgc aaccgatgtt
     1441 caagggggac atcgtggagc agggccgcaa gctgaccgga atgatcaaca ccgtggtggt
     1501 caagctcgac cagctcggtg acatcgttcc ggcggtacag gatctcggca agaggcatgt
     1561 tgcctatggc gtcaaggacg agcactacgg cgtcgtcgcc ggcgcgctcc tgtggacgct
     1621 gggcgcgggc ctgggcgacg ctttcacgga cgacgtcagg caagcgtgga ccgccgccta
     1681 cacgatcctg gcaggcgcga tgaaagacgc agccgcgacc gtccaggcct gaatcggacg
     1741 gggcgagtgg cgccttgcgg cgccactttt tcgcgcattc ccatctcagt gggccattgc
     1801 gctgcgcgcc acccgcgacg tatgaaatcg cgcggccagt gcgggtgcca ccgcggaacc
     1861 ggctttgccg ggccgctggt gggccttgca ggccgcatgg ccgcgagacg cggccattcc
     1921 tcgacctgtc caaaccgacg caggtcggtt tggagccgca ggtctcggcc ccattgaggg
     1981 ggagagggcg aagcccgagg gggcatcgcc gaaggcgctt cgggggtggg ctttcacatc
     2041 aggcgtgatc gcactcgttc aggaagtcga gcaattgctc cctgagctgg tagtagtcgg
     2101 gatgctcgag cagcgccttg cgggtgcgtg gacgcggcag atcgacctcc aggaccttgc
     2161 cgaccctggc gcgcgggccg ttggtcatca tgaccacgcg gtcggccagc aggatcgctt
     2221 cgtccacatc gtgcgtgacc atgatcgccg tgagcttggt gcgagcccac acttccatca
     2281 atacttcctg caggtcccag cgcgtgagcg aatccagcat gccgaaaggc tcgtccagca
     2341 gcagcagctt gggcgagagc gcgaaggcgc gcgcgatgcc cacccgctgc ttcatgccgt
     2401 tcgaaagttc cagcgccttc ttgtgggccg cgtccgccag cccgacccgt tccaggtagt
     2461 gctcgatgat gtccttgcgc tccttcttgc tggcgtgcgg gaacaccttc tccacgccca
     2521 ggccgacgtt ctggtacgcg gtcagccacg gcaccaggct gggcgcctgg aacacgatgc
     2581 cgcggtccgg gccggcggtc atcacctccc tgccgtccag gaagatgcct ccttcgctga
     2641 cgtcggtcag gccggcgatc atcgacagca cggtcgattt cccgcagccg gaatggccga
     2701 tcacggaaac gaactcgccc ttgcggatct tcaggtcgaa ctgctcgacc accttgaccg
     2761 ggcccttgcg gctcggatag atcttggaca ccgcggagaa ctcgacgtag cgttccgccc
     2821 gcaccggtac ctgctcgggt gcgacgtccg gcttggcggc ctgccgatgc tcgctgtcgg
     2881 tgttcggcct cacgcttggg aggtgaatga cgttgccgcc atcgccggca ctgttttgcg
     2941 cgccggcatc catcaggtaa cgcgtgaccg acttgcgaat ccgcttgaag tcttccgaat
     3001 gattcatgcc ggcgcggtcg cgcgggcgcg ccagcgtcac cgggaattcg ggaccgaaag
     3061 tggcaccggg accgggattg agcgggatga tgcggtccgc catcatgatg ccttcatcga
     3121 catcgttggt gatgaggatg acggtcttct tctcctggct ccagatgcgg atgatttcgt
     3181 cctgcagttt ggcgcgggtc agcgcgtcca gcgcggacag gggctcgtcc agcaagagga
     3241 tgtccggatt ggcggcgagc gcacgcgcca ccgcgacacg ctggcgcatg ccgccggaca
     3301 gttccgccgg acgcttttcc agcgccgggg tcaggttgac catgtcgatg tacttgtgca
     3361 ccctttccgc gcgctgcgcc ttcggggcgt ccttgtatac ctggtcgacc gcaagcgcga
     3421 cgttgtcgcg caccgtcatc cacggcatca acgagtagct ttgaaacacc acgccgcggt
     3481 ccggccccgg accggcgatc ggcgcgccgt tgtgcaggat ctcgccctca tcggctgcga
     3541 tcaggccggc gatcagcgaa atcagcgtgg tcttgccgct gccggagaag ccgacgatgg
     3601 ccacgaattc gccttgcctg atctgcaggt tgatgtcttt cagcacctgg gtccggtgcg
     3661 cgccggcgcc gtaggacttg ccgaggtttc tgagctccag gcagtacccg cccgggctgc
     3721 tggccgggcg ctccgcgtcg cgctggacgt gcggcacgct cgtcgcggcg gcaggggcga
     3781 tttcgaggaa cgccgttgac ggtgggactt tcaatgagta ggcagtcatg gcggttcccc
     3841 ggtcaaacgc ggtcgccgaa actcaccagc tgctgcaggg tgtgcatgat ccggtcgagc
     3901 aggaagccga cgagcccgat ggtgaacacc gccaccatga tgcggccgag ggagttggag
     3961 ctgccgttct ggaattcgtc ccacacgaac ttgcccaggc cggggttttg cgccagcatc
     4021 tcggcggcga tcagcaccat ccagcccacc ccaagggaca ggcgcaggcc cgtgaaaatg
     4081 aacttgagcg aggagggcac gacgatcttg agcaccttgg tggtccagga caggcgcagc
     4141 accttcgaca cgttgatcag atccttgtcg atcgacgaca cccccagcgc ggtgttgatc
     4201 agcgttggcc acaatgaaca cagcgtcacg gtgatggccg agaccaggaa ggatttctgg
     4261 aaccatgcgc cgtcggaggt cacatacgcc gcgctcacga ccagcgtcac gatcggcaac
     4321 cacgcgagtg gcgataccgg ccggaaaatc tggatcaagg ggttcagcgc tgcctgcacg
     4381 gtgcggctca aaccgcacaa tatgcccacc ggaaccgcta ccagcgtggc gatcaggaag
     4441 ccggtgaaca cggtcttcag gctcgtgagg atctggtcga agtaggtcgg cttgccggtc
     4501 cacggccgga tggtggtgtc ggctttcgga tcggacttca gtgcctcggc ccgctgcgct
     4561 ttctggcgct caaggaaggc agccgctttg tcccgctcgg cgtagtgttc ggcgatcagg
     4621 ct
//
LOCUS       DASWIY010000448         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_681460, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000448 DASWIY010000000
VERSION     DASWIY010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681460"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VGA59_15970"
     CDS             complement(<1..587)
                     /locus_tag="VGA59_15970"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX9721216.1"
                     /translation="MKLHPFLRSTAAVAFCGMVGLAAAQASPQIVGEATMVIGVARVF
                     SLDGTTRAMERGAAIRVGDRVETEAGGHVHFRFVDGGRLSIRPLSRLQVESYNHSSQE
                     PALTAIKFRLDEGVVRSITGSWGEAARDKFRLNTPVAAIGVKGTDFVVRSDAEKTNAS
                     VYTGAIVLSPLTAGCAATLGPCQSGREKLLSDDMKG"
     gene            complement(944..>2423)
                     /locus_tag="VGA59_15975"
     CDS             complement(944..>2423)
                     /locus_tag="VGA59_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9721217.1"
                     /translation="VYVARDEAHAQAQAPGKTLRRDEDVLDTWYSSALVPFSTLGWPD
                     KTREMDLYLPSSVLVTGYEIIFFWVARMIMMTTHFTGQVPFRDVYIHGMVRDSEGKKM
                     SKSEGNVVDPVDLIEGADLDTLVHKSTVGLRRPETAPKVAARVKKEFPHGMPAYGADA
                     LRFTMASYASLGRNINFDTKRCEGYRNFCNKLWNAARFVLMNCEGHDLAAQGGTLTEA
                     DHWIISTLQRAEADVAKGFAEYRLDNVANTIYQFVWDEFCDWYLEIAKVQIQTGDAPQ
                     QRGTRRTLIRVLEAILRLAHPVIPFITEELWQNVAPVAGKSGDSISVAPYPATDSAAV
                     HPESEAYVAQLKSVVDACRNLRGEMNVSPSTKLPAYVVGDAAFIRRAAPVLQALARLS
                     EVRVFDEEAAWAAAAKAAPVAVVGEARLCLYMEIDLGAEKSRLGKEAARLQGELAKAN
                     GKLSNEAFVAKAPAAVIEQERRRIAEFSATLGKIQDQLARLG"
BASE COUNT          399 a          800 c          792 g          432 t
ORIGIN      
        1 cccttcatgt cgtcggacaa cagcttttcg cggccgctct ggcagggacc gagcgtcgca
       61 gcgcagccgg cggtcagggg ggacagcacg atggcgccgg tgtaaaccga agcattcgtt
      121 ttttcagcgt ccgacctgac cacgaagtcc gttcccttga cgccaatggc ggccacgggt
      181 gtgttgagac ggaacttgtc gcgcgcagcc tcgccccatg agcccgtaat ggagcgcacc
      241 acgccttcgt cgagacggaa cttgatggcc gtcagggcgg gctcttgcga tgaatggttg
      301 taactttcca cctgcagccg gctcaagggg cggatgctga ggcggccccc gtccacgaag
      361 cggaaatgga cgtggccgcc ggcctcggtt tcgacgcggt cgcccacccg gatcgccgcg
      421 ccgcgctcca tcgcccgggt ggtgccatcg aggctgaata ccctggccac accgatgacc
      481 atggtcgcct caccgacgat ctggggcgac gcttgcgcgg cagccagccc caccatgccg
      541 cagaaggcca ctgcggcagt ggagcgcaga aacggatgaa gcttcatgaa aacacctctc
      601 aatgggcgag caccacagga cgctgcggag ccaggccgac ggcggatttt ctcacgtccg
      661 ccagagccga cttgtgcgcc cggtcacatt ttcgcgattc ggagccagca gtatgcgcgg
      721 cggaatggga cttgtcatgc ctgggcttga cccggcatcc atgacttcgt ggattgcgga
      781 tcaagtccgc aatgacaatt cgtttaggct tgacccggga tccatgtatc agggtcatgg
      841 attgcggatc aagtccgcaa tgacagagag agggctgtca tcccggggcc gcaaatgaca
      901 gaaagagggc tgtcatcccg gggccgcaat gacaggatca aaatcacccc agccgggcca
      961 gctggtcctg gatcttcccg agggtggcgc tgaactccgc gatgcgccgg cgctcctgct
     1021 cgatcacggc tgccggcgcc ttggccacga acgcctcgtt ggagagcttg ccgttggcct
     1081 tggccagctc gccctgcagg cgggccgctt ccttgcccag gcgcgacttc tcggcgccga
     1141 ggtcgatttc catgtaaagg cacaggcgcg cctcgccgac cacggccacg ggcgcggcct
     1201 tggcggcggc ggcccaggcg gcctcttcgt cgaacaccct gacctcgctc agtctggcca
     1261 acgcttgcag caccggcgcc gcccgccgga tgaaggccgc gtcgcccacg acataggcgg
     1321 gcagtttggt ggacggcgac acgttcattt cgccgcgcag gttgcggcag gcgtccacca
     1381 ccgacttgag ctgcgccaca tacgcttcgg actcggggtg gacggcggcg ctgtcggtgg
     1441 cggggtaggg cgcgacactg atggagtcgc cgctcttgcc cgcgaccggt gccacgttct
     1501 gccacagctc ctcggtgatg aacggaatca ccggatgcgc cagccgcagg atggcttcga
     1561 gcacgcggat cagcgtgcgg cgggtgccgc gctgctgggg cgcgtcgccg gtctggatct
     1621 gcacttttgc gatctccagg taccagtcgc agaactcgtc ccagacaaac tggtagatcg
     1681 tgttggcgac gttgtccagg cggtactcgg cgaagccctt ggccacgtcg gcctcggcac
     1741 gctgcagcgt ggagatgatc cagtggtcgg cttcggtcag cgtgccgccc tgcgcggcca
     1801 ggtcgtggcc ttcgcagttc atcaggacga agcgcgccgc gttccagagc ttgttgcaga
     1861 agttgcggta gccctcgcag cgtttggtgt cgaagttgat gttgcgcccc aggctggcat
     1921 agctcgccat cgtgaagcgc aacgcatcgg cgccatacgc cggcatgccg tgcgggaatt
     1981 ccttcttcac ccgcgccgcc accttgggcg cggtctcggg ccggcgcagc cccaccgtgg
     2041 acttgtggac cagcgtgtcc aggtcggcac cttcgatcag gtccaccggg tccaccacgt
     2101 tgccttcgga cttgctcatc ttcttgcctt cgctgtcgcg caccatcccg tggatgtaga
     2161 cgtcgcgaaa gggcacctgc ccggtgaagt gtgtcgtcat catgatcatc cgggcgaccc
     2221 agaagaagat gatctcgtag ccggtcacca gcaccgacga cggcaggtac aagtccattt
     2281 cccgggtctt gtcgggccag ccgagcgtcg agaacggcac cagcgccgag gagtaccagg
     2341 tgtccaggac gtcttcgtcc cgccgcagcg tcttgcccgg ggcctgcgcc tgcgcatgcg
     2401 cctcgtcccg cgcgacgtag acc
//
LOCUS       DASWIY010000449         4442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2913092, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000449 DASWIY010000000
VERSION     DASWIY010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2913092"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="VGA59_15980"
     CDS             complement(<1..1495)
                     /locus_tag="VGA59_15980"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX9721218.1"
                     /translation="MNCIKWFKWMFLLLALPAVHAAPPHLDLEVTLDPHTRKFRAKAE
                     LTTSAGRPQLALDPLFKVSRLTVNGVNASSVAGAQRALSRQATRASQRLSMEYSGTLP
                     ALPEGRQRNSPNPAALFASPGGSYLSGGAGWYPDPGVPFTYRARLSLPQGQKALAPGK
                     QVRQDESGAGYVAEYEFAHPAEGIWVMAGPYQVAQRTVVLDDGKPVTVRTWFHPELSG
                     LADDYLQDSARYIQRYSRLIGAYPFGDFSIVSSPLSHGLGMPSLTYLGRDVLRLPFIR
                     ATSLGHEVLHNWWGNGVYPEWSRGNWSEGLTTFMADYAYREDQSEQAAREMRLTWLRD
                     LTGIAASNETALVDFKSRHHGISSVIGYAKSAMLFFMLRDEIGRPAFEQGLRLFWQRH
                     QFKTANWKDLQAVFAQAGGRDLSGFFAQWTARASSPQLLLVPTQAPAQAGGVKLVQQG
                     DVFDLLVPLRISTASGETQEIKVRVRERETAVDPATLPPGARAVELDP"
     gene            complement(1522..2439)
                     /locus_tag="VGA59_15985"
     CDS             complement(1522..2439)
                     /locus_tag="VGA59_15985"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9721219.1"
                     /translation="MAAAIQLTPLIMSPSLFQFTTMANIAFLGTGLLGGAFAEAAAKR
                     GDSVTAWNRSPAKVQSLAQFGVKAAGTPAEAVRGASSVHLVLKDDAVVEEVISAARAG
                     LSPEAVLIDHTTTLPALTAARAERLRAEGVKYLHCPVFMGPPAARNALGTMMASGPRA
                     LFESVQADLARMTGRLEYLGERADLAAVNKLFGNAMIIGVSALMADILTLALASSVAP
                     QEVIKLLGLLDLNAMVAGRGANMAKANFTPSFELAMARKDVRLMLETAGGRPMAALPA
                     VAARMDELIASGHGAQDASVLAIDAVRGA"
     gene            complement(2418..2813)
                     /locus_tag="VGA59_15990"
     CDS             complement(2418..2813)
                     /locus_tag="VGA59_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9721220.1"
                     /translation="MNSVMANWAPRLLSVLRMVSAFMFVQHGTQKWLAFPVPRPTPTE
                     FWSLSGAAGLLELAGGALLLLGLFTRPVAFVLSGLMAFAYFIAHAPQGFWTIANRGEL
                     AVLYCFVFLYFAAAGGGPWSVDRWLRRSN"
     gene            3047..>4442
                     /locus_tag="VGA59_15995"
     CDS             3047..>4442
                     /locus_tag="VGA59_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015514 - nitrite efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015707 - nitrite transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HEX9721221.1"
                     /translation="MHDRQHPQTASVAGPRRGHVLTLWTPDDKSFWEREGEAVAKINL
                     WISVPALFLAFAIWQVWSVVAVGLPSLGFKYSTNQLFWLAAAPALSGATLRIFYSFIV
                     PVVGGRRWTAISTASLLIPAIGIGFAVQDNTTTYPTMLMLALLCGLGGGNFSSSMANI
                     SFFFPKERKGSALGVNAGLGNLGVSVVQFLSPLVITAGVFGIFGGDAQTIVKDGQTVQ
                     VWTQNAAFIWVPWILLASLAAWFGMNDIADVKASFATQATIFRRKHNWLMCLLYLGTF
                     GSFIGYAAGFPLLIKNQFPGVNPLAYAWLGPLVGALIRPFGGWLSDKLGGAKVTFWNF
                     VVMLLAVIGVLYFLPSGASGGSFTGFFLMFLVLFLTTGIGNGSTFRMIPVIFQNEALA
                     RIKPVDDESRAQALKEGNTEGAATLGFTAAMAAYGGFFIPKSYGTSITMTGGPEAALY
                     TFIVFYALCIAVTWW"
BASE COUNT          738 a         1475 c         1452 g          777 t
ORIGIN      
        1 cggggtcgag ctccactgcc cgggcccccg gcggaagggt cgccgggtcg acggcggttt
       61 cccgctcgcg cacgcgcacc ttgatctcct gggtctcgcc cgaagccgtg ctgatgcgca
      121 ggggcaccag caggtcgaaa acatcgcctt gctgaaccag cttcacgccg ccggcttgcg
      181 ctggagcctg cgtgggaacc agcagcagtt gcggagaaga ggcacgagcc gtccattgcg
      241 cgaagaaccc cgacaggtcg cggccgccgg cctgggcgaa cactgcctgc aggtctttcc
      301 agttcgcggt cttgaactgg tggcgttgcc agaacagccg caggccttgc tcgaacgccg
      361 gccggccgat ctcgtcgcgc agcatgaaaa acagcatcgc cgatttggca tagccgatca
      421 ccgaggaaat accgtggtgg cgcgacttga agtccaccag ggctgtctcg ttcgacgccg
      481 cgatcccggt caggtcccgc agccaggtca ggcgcatctc gcgggcggct tgctcggact
      541 ggtcctcccg gtaggcgtag tcggccatga aggtggtcag cccctcggac cagttgccgc
      601 gcgaccactc ggggtagacg ccgttacccc accagttgtg caacacctcg tggcccagcg
      661 aagtcgcgcg aatgaaaggc aggcgcagca cgtcgcgccc gaggtaggtg aggctgggca
      721 tgcccaggcc atgcgacaac gggctggaaa cgatgctgaa gtcgccgaaa gggtaggcgc
      781 cgatcaggcg gctgtaccgc tggatgtagc gggcgctgtc ctgcaggtaa tcgtcggcca
      841 ggccggacag ttcggggtga aaccaggtcc gcaccgtgac cggcttgccg tcatccagca
      901 caacggttcg ctgcgccacc tggtaggggc ctgccatcac ccagatgcct tcggcggggt
      961 gagcgaattc gtactccgcc acgtacccgg cgccgctctc gtcctgccgg acctgcttgc
     1021 cgggagcgag tgccttctgg ccttgaggca gcgacagcct cgccctgtaa gtgaacggaa
     1081 cgcccgggtc cgggtaccag cctgcaccac ccgacaggta gcttccgccg ggtgaggcaa
     1141 acagcgcggc aggattgggc gagttgcgtt gccggccttc aggcagcgcc ggcaaagtgc
     1201 ccgaatattc catgctcagg cgttgcgatg cgcgggttgc ctggcggctc aatgctcgct
     1261 gggcgccggc tacggagctt gcattcacgc cgttcaccgt cagacgcgaa accttgaaca
     1321 gcgggtcgag cgcgagctgc ggccggccgg cgctagtcgt cagttccgcc ttggcccgga
     1381 attttctcgt gtgggggtcg agggtgactt ccagatccag gtgcggtggc gcggcatgca
     1441 cggcgggcag cgcgagaagg aggaacatcc acttgaacca cttgatgcag ttcatccggg
     1501 gcctcatcga tcgacgccgg ttcaggctcc acgcacggcg tcgatcgcca gcacgctggc
     1561 atcctgggcg ccatgccctg acgcgatgag ctcatccatc cgggccgcga ccgcgggcag
     1621 cgccgccatg ggacgcccgc ctgcggtctc cagcatcagc cgcacatcct tgcgcgccat
     1681 ggccagctcg aagctggggg tgaagttggc cttcgccatg ttcgccccgc gtcccgccac
     1741 catcgcgttc aggtcgagca acccgagcag cttgatgact tcctgagggg ctacgctgct
     1801 cgccagcgcc agcgtgagga tgtcggccat cagggccgag acgccgatga tcatggcgtt
     1861 gccgaacagc ttgttgaccg cggcgagatc ggcgcgttcg cccaggtact cgaggcgccc
     1921 ggtcatcctg gccagatcgg cctgcacgct ttcgaaaagc gcgcgaggtc ccgaggccat
     1981 catcgtgccc agagcgtttc gcgcggccgg cgggcccatg aagacggggc agtgcaggta
     2041 cttcacccct tcggcgcgga gccgctcggc ccgtgcggcg gtgagtgccg gcagggtcgt
     2101 ggtgtgatcg atcaggaccg cctcggggga gagtccggcg cgcgcggccg agatgacttc
     2161 ctcgaccacg gcgtcgtcct tgagcacgag atgcacgctg gatgcgccgc ggaccgcctc
     2221 ggctggcgtg ccggcggcct tgacgccgaa ctgagccagg ctctgaacct tggccggtga
     2281 acgattccac gcggtgacac tgtcgccccg cttggcggcg gcctccgcga atgcaccgcc
     2341 caaaagtcct gtgccgagaa atgcgatgtt tgccatggtc gtaaactgaa aaagcgacgg
     2401 tgacattatg agtggcgtca attggatcgc cgcagccatc ggtccacact ccatgggccg
     2461 ccgccggcag ccgcgaaata gaggaagacg aagcaataga gcacggccag ctcaccccgg
     2521 ttggcgatgg tccagaaacc ctgaggtgcg tgcgcaatga aataggcaaa ggccatcagg
     2581 cccgacagca cgaacgccac cggccgggtg aacagcccca acagcaacag agcgccgccg
     2641 gcaagctcga gcaggcccgc ggcgccgctg agcgaccaga actcggtcgg tgtgggcctt
     2701 ggcaccggaa atgctagcca cttctgtgtt ccgtgctgca cgaacatgaa cgccgagacc
     2761 atgcgcagca cgctgagcag ccgaggtgcc cagttcgcca taacggaatt catgctggtt
     2821 cgagccatgc cggcaggcct ggttccaccc gtgcttcagc aatgggtcac ccatcgggaa
     2881 tgacttccgt ttcgcatggc atcgcgtgcg cgcggtcgct gaaactctct gtcacgatat
     2941 tgaattcacg gtcgagcact cgggcgtcgc gtggcgcgct agattcgaag cttttctgcc
     3001 gaggcacgtc ccggcaccgg gagacggacg acaagaagaa ccgccgatgc acgatcgtca
     3061 acatccgcaa accgcatctg tcgctggccc gcgccgtggt catgtgctca ctttgtggac
     3121 cccggacgac aagtccttct gggagcgcga gggcgaggcc gtcgccaaaa tcaacctgtg
     3181 gatcagcgtg ccggcgctgt tcctggcgtt cgccatctgg caggtctgga gcgtggtcgc
     3241 cgtcgggctg ccctcgctcg gcttcaagta ctcgaccaac cagctgttct ggctggccgc
     3301 tgcgcccgcg ctgtccggcg ccacgctgcg catcttctat tccttcatcg tcccggtcgt
     3361 cggcgggcgc cgctggaccg cgatctccac ggccagtctc ctgattcccg ccatcggcat
     3421 cggcttcgcg gtgcaggaca acaccaccac ctacccgacc atgctgatgc tggcgctgct
     3481 gtgcggcctg gggggcggca acttcagctc cagcatggcc aacatcagct tcttctttcc
     3541 gaaggagcgc aagggctcgg ccctgggcgt caatgccggg ctgggcaacc tgggcgtgtc
     3601 ggtggtgcag ttcctcagcc cgctggtgat caccgccggg gtcttcggca ttttcggggg
     3661 cgacgcgcag accatcgtca aagacggcca gacggtgcag gtgtggaccc agaacgcggc
     3721 cttcatctgg gtgccgtgga tcctgctggc ttcgctggcg gcctggttcg gcatgaacga
     3781 catcgccgac gtcaaggcct cgttcgcgac ccaggcgacc atcttccggc gcaagcacaa
     3841 ctggctgatg tgcctgctct acctgggcac cttcggttcc ttcatcggtt acgccgccgg
     3901 ctttccgctg ctgatcaaga accagttccc gggcgtgaac ccgctggcct atgcctggct
     3961 gggcccgctg gtcggcgcgc tgatccggcc cttcgggggc tggctgtccg acaagctggg
     4021 cggcgcgaag gtcacgttct ggaatttcgt tgtcatgctg ctggccgtca tcggcgtgct
     4081 ttacttcctg cccagcggcg cctcgggcgg cagcttcacc ggcttcttcc tgatgttcct
     4141 ggtgctgttc ctcaccaccg gcatcggcaa cggctccacg ttccgcatga tcccggtgat
     4201 cttccagaac gaagcgttgg cccggatcaa gccggtcgat gacgaatcga gggcacaggc
     4261 cttgaaggaa ggcaacaccg aaggcgcggc gacgctgggc ttcaccgccg ccatggcggc
     4321 ctacggcggc ttcttcatcc ccaagagcta cggcacctcc atcaccatga ccggcggccc
     4381 ggaggcggcg ttgtacacct tcatcgtctt ctacgcgctg tgcatcgccg tcacctggtg
     4441 gt
//
LOCUS       DASWIY010000450        20581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3612021, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000450 DASWIY010000000
VERSION     DASWIY010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 20581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20581
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3612021"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..101
                     /locus_tag="VGA59_16000"
     CDS             <1..101
                     /locus_tag="VGA59_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HEX9721222.1"
                     /translation="KRKRPESFKPLQDRFGNPASVTTEKKFGGGNE"
     gene            94..804
                     /locus_tag="VGA59_16005"
     CDS             94..804
                     /locus_tag="VGA59_16005"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HEX9721223.1"
                     /translation="MSSEGFLGRWSKRKLEVKKAKAVEAEPTPELPPAPQPSPASGRG
                     SQTGSQSRLASGRGSEAAITEAATGLAPSPPGGELARGPGGGEGPVQAPLPPTIEDVE
                     ALTPESDFTRFAAPDVTPEVKNAALKKLFADPRYNVMDGMDVYTGDYSKPDPIPQAML
                     RQLAGAKFLGLFREEERQEKAARDARDDADNHTHQKVAQSGSESQAVPEPPEHADPDL
                     QLQQDHAAPGEDPGRGTG"
     gene            731..2812
                     /locus_tag="VGA59_16010"
     CDS             731..2812
                     /locus_tag="VGA59_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9721224.1"
                     /translation="MPTLICNCNKTMPLQEKTLGAALGEELILHTTLCRREAGAFQQA
                     VKSGADVVVACTQEQRLFAEVGQETAGAVSPVKFVNIRETGGWSRDASRAMPKIAALL
                     AAAHLPDPEPVATVSYKSAGKLLVIGPMEQAERAAAFVSDALDVTVFTQGGTGGQERR
                     FPVLGGRIESLKGWLGAFELQWSRNNPIDLDLCTRCNACVAACPEGAIGLDYQIDLAK
                     CASHRECVKVCEAAGAIDFSRESETDTEKFDLVLDLRAAPAFLQHAPPQGYFHLPASQ
                     GDMLGAVLKLRELVGEFEKPRFFGYKQKLCAHSRNETVGCNACIDICSAEAILSDKSR
                     QQIKVNPNLCVGCGACTTVCPTGALTFAYPRASEQGLKFKTLLSTFRQAGGKDAVLLL
                     HSQERGQALVEEVGRAAQLGVAHGVPANVIPVALWHTASLGLDVWLSAIAFGASRIAV
                     LLTDEEALQYREALREQMEVGEAILNGLGYGGSHFKLMHAHRLADLDQELQLLLAQPP
                     VVPAAAARFAVAPEKRATLELALDHLVENAPARPEAIELPAAGAAFGTLEVSKAKCTL
                     CLSCVSACPASALQDNPELPQLRFIEKNCVQCGLCATTCPEDAIVLRPRLLLTRERNE
                     ARVLNETRPYACVRCGKPFGTLKAIEAMLGKLAGHSMFQGEALERLKMCADCRVIDMY
                     SARNEVKITDL"
     gene            2813..3442
                     /locus_tag="VGA59_16015"
     CDS             2813..3442
                     /locus_tag="VGA59_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX9721225.1"
                     /translation="MHPNTSERIPVSSALDEETARAEVYGLLAQLYYASATPELLSAI
                     RAAVTEAPAAGAFLEEPWREVVGLARSMDDAAVAAEYDSLFGGVGKPEVYLFGSHYLS
                     GFLNDRPVARLRSDLAALGLERDGTVTETEDHIACLCEVMRYLIAGDDVAVANLTRQR
                     EFFATHLQPWVPTLCDTIARHPKARFYATLAGFTQAFIGVEIQGFDMLD"
     gene            3584..3790
                     /locus_tag="VGA59_16020"
     CDS             3584..3790
                     /locus_tag="VGA59_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEX9721226.1"
                     /translation="MSQPTGKLSRRHLFVGAGTAGAVAAAASLVPGAVQETVATPQAK
                     EPPARGGGYQLTEHVKLYYKTTLV"
     gene            3815..6781
                     /locus_tag="VGA59_16025"
     CDS             3815..6781
                     /locus_tag="VGA59_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX9721227.1"
                     /translation="MLLTRKMGAAQAAARSPFVHSLQRGLARALPTMDRRAFLRRSGL
                     GVGVGIAASSLTLIKKAKAAEGKATSVGQGKIEVKRTVCTHCSVGCAIDAVVENGVWV
                     RQEPVFDSPLNLGAHCAKGAAIREHGHGEFRLRYPMKLVNGKYERITWDTALNEISAK
                     ILDLRKASGPDSIYWIGSSKHSNEQSYLMRKFVSLWGSNNCDHQARICHSTTVAGVAN
                     TWGYGAMTNSYNDMQNSKCALYIGSNAAEAHPVSMLHMLHAKETGTKMIVVDPRFTRT
                     AAKADEYVRIRSGSDIPFLFGLLHHIFKNGWEDQKYINDRVYGMDKVKADVMAKWTPD
                     KVEEACGVPEAQMAKVAEMMAKNRPSTIVWCMGQTQHTIGNAMVRASCILQLALGNIG
                     VSGGGANIFRGHDNVQGATDVGPNPDSLPGYYGIAEGSWKHFAKVWGVDFDWIKARFA
                     NPGMMSKPGITVSRWIDGVMEKNELIDQDPNLRGVVFWGHAPNSQSRGLEMKRAMDKL
                     DLLVVVDPYPSATAAMAAMPGKPEDLNPNRAVYLLPAATQFETSGSVTASNRSLQWRE
                     KVIEPLWESRSDQMIMHQLAEKLGFGKELSQNFKMQKVKGMDEPVPEEILREINRSVW
                     TIGYTGQSPERLKAHMRNMANFDVKTLKSKGGKDKETGYDLTGDYFGLPWPSYGNPEL
                     KHPGSPNLYDTSKHVMDGGGNFRANFGVEKDGVNLLAEDGSASMGADIQTGYPEFDHV
                     LLKKLGWWNELTDVEKAAAEGKNWKTDSSGGIIRVTMKNHGCHVFGNAKARAVVWNFP
                     DGIPQHREALYSTRPDLVAKYPTHDDKKAFWRLPTLYKTLQQQNITDKVAEKYPLILT
                     SGRLTEYEGGGEETRSNPWLAELQQEAFVEINPKAAAERGIRNGDRVWLMTPTGARLN
                     VQALVTERVGPDTCWMPFHFSGRWQGVDMLPYYPSGAAPVVRGEAVNTATTYGYDSVT
                     MMQETKTTICNVEKAA"
     gene            6874..7491
                     /gene="fdh3B"
                     /locus_tag="VGA59_16030"
     CDS             6874..7491
                     /gene="fdh3B"
                     /locus_tag="VGA59_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HEX9721228.1"
                     /translation="MARMKFICDSERCIECNGCVTACKNEHEVPWGVNRRRVVTLNDG
                     IPGERSISVACMHCSDAPCMAVCPVNCFYRTDEGVVLHDKDVCIGCGYCSYACPFGAP
                     QFPAAGTFGVRGKMDKCTFCAGGPEAHGSQAEFDKYGRNRLAEGKLPACAEMCSTKAL
                     IGGDGDVVADIFRNRVIKRGKGAEAWGWGTAYGSRQAGQPAGGKS"
     gene            7488..7727
                     /locus_tag="VGA59_16035"
     CDS             7488..7727
                     /locus_tag="VGA59_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721229.1"
                     /translation="MKRKLILAAAVVALAACTDKPQTLESGVRATAAGPDSAAFQGTG
                     MPFAAPGWKPGDKVSWEQHLKTRTQNGQNDYTRVN"
     gene            7738..8931
                     /locus_tag="VGA59_16040"
     CDS             7738..8931
                     /locus_tag="VGA59_16040"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX9721230.1"
                     /translation="MLRSLLLTFALAASLPAFAQTKAPDDPAPGATAPAAPGGIQSQN
                     IFDVKPEVKPDASSDPDYMKQNNAERNRVQPGNNAPIWRGVVSGMEGFTSLPKSQAPE
                     AGVLIQAPVQYPGSRMTTAGEAWRQVRNNWIIPYGGALFLIGLGALAIFYFARGPLGG
                     DAESGRKIERFTPFERSAHWANAIAFCILAISGLVMAFGKFFLLPVMGGTLFGWLTYV
                     LKNLHNFAGPVFAVSLVIVIVTFMRDEWPQRGDLRWLLRIGGAFDKNGHQPPAANRFN
                     AGEKVIFWFGVFLLGIIVVGSGLVMDMLIPNVVYERGTMQVTNMIHGVAAVLMMTLFL
                     LHIYLGTIGMRGAYMGMRTGYVDEAWAREHHSYWYEDIKAGKIPVQRSQPTAIADGAE
                     TARPA"
     gene            8954..9436
                     /locus_tag="VGA59_16045"
     CDS             8954..9436
                     /locus_tag="VGA59_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721231.1"
                     /translation="MKTLLVSTVLATACALAWAKLPPPDDAAKAKAAETAAKSAWQGK
                     VDAHLLCKAQDKVAAYYMKTAAKKPAPAAAPSAAAAPAAAAPAATASAAAAPAQPAAS
                     GTPVAAKPPPPCADPGPFAYNPPEQKPLETAGAHSPSGTAASPPSVKPPSAEMAPAKK
                     "
     gene            9602..10447
                     /locus_tag="VGA59_16050"
     CDS             9602..10447
                     /locus_tag="VGA59_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX9721232.1"
                     /translation="MPLPFLTEARAPLTQEIATVNEYGERDTVSVPAERPLTVYVDKR
                     ELVTLMTLGARPEWLVLGYLRNQRLVNALDEIESITVDWEVGAAAVNTRCGIERIEER
                     TAKRVVTTGCGQGSVFGGLMDEIESISLPQASITQSQLYGIVNAIRLQESTYKSAGSV
                     HGCALFRGEGMLLFVEDVGRHNAIDTIAGWMWLQDPPMESNDKVFYTTGRLTSEMVMK
                     SAQMGVPIVISRSGITQMGHAIAQRLSLCAIGRATNKRFLCYSAPQRLQLQPELAGPK
                     LRAAA"
     gene            10613..12094
                     /locus_tag="VGA59_16055"
     CDS             10613..12094
                     /locus_tag="VGA59_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEX9721233.1"
                     /translation="MMPPTDDAPFPTPATAAAPAAPERLPLRVWLREGLRAGFFLPPR
                     IAGEQPTPLQVAALLLIFSLLEIGLARLEVAGAAHFDLRGWLAPWWSTAALLLLAWWV
                     LPPIAPGAPGRPAGLAAWFVLWLSAVLPVNTVSQLLGIAQAHEMLPQQLETSAWAAWV
                     LYLLLWAWTVGVVLRMTAWFGAGLPRLGVLALGMVGLFGLAAVQFPDRPWQAEAPGDK
                     RPRLELSQELFEAQRALWQETMSGIAPDRAGTVDVYALVFAPYAGEDVFLRESGMVAK
                     LLTERFDAGGRVVHLANHPLTARTIPWATQLNLERAVEALAARMDREHDVLVVYLTSH
                     GAADFKLAAAHGPLEVKPVGPADLRRALDRAGIRNRVIAVSACYSGGWVGPLASETTL
                     VMTAADVDRTSYGCGRRSELTFFGRAVFDEQLRQTHSFEQAFAAAVPLIRQREIEAGK
                     EDGFSNPQISVGEKIRPVLRDLERRLGTAAAGAGANPGLSLVR"
     gene            12104..14626
                     /locus_tag="VGA59_16060"
     CDS             12104..14626
                     /locus_tag="VGA59_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9721234.1"
                     /translation="MTATSSWQLGWRTLWRDLRAGELRLLIVAVTLAVAALTAVGFLA
                     DRLKGGLVRDARQLLGGDAVISSDNPTPQAFIDQARALGLRSLSTLSFPTMARAPDAR
                     GGASRLVALKAVPAGYPLRGNLKVAPQPEAPGADTRDIPAPGEAWVDAPLLDMLGLKV
                     GDPVLLGDAQLRVARIIVLEPDRGTGFVNFAPRVMINEADLAATRLIQPASRVGYRFA
                     VAGADPRVSMFARWAAQEVKSPAVRGVRLESMESGRPEMRQTLDRAEKFLNLVALLAA
                     LLSAVAVALAARGFAGKHLDDCAMLRVLGQSQRTIALSYTFEFALVGIAASALGVALG
                     YSVHFVFLQLLAGLVEATLPAATLWPVAFGMGMGLTLLFAFGLPPVLQLAQVPPLRVI
                     RRDLGNLKPASLSVLAIGVAGFAALLLAASSDLKLGAIAVGGFAGAALLFAGLSWLAV
                     KLLRLSVNEATAPRWMVLATRQISARPAYAVVQVSALAVGLLALVLLVLLRTDLVASW
                     RRATPPDAPNRFVINVMPEQSETFQKMLRGAGVQRFDWYPMIRGRLVAVNGRAVTPDD
                     YPEDRAKRLLDREFNLSTTTVQPPHNQVVAGRWQSEEQGAVSVEEGIANTLGLKMGDT
                     LRFDIGGMQEDAKITSLRKVDWGSMRANFFVIYPVTRLQGVPVTYMSAFRAPDRKGFD
                     NELVRAFPNITTVDTTSTINQVQGVLDKVIRAVEFLFGFTLAAGVVVLFAAVTATREE
                     RAREFAIMRAVGASSALLRQVQRAELVGVGLLAGFLASVVASAVGWGLARFVFDFDWT
                     ASAWVPLVGALAGAALALAAGWWGLREVLNRPVIETLRRAPE"
     gene            14934..15872
                     /locus_tag="VGA59_16065"
     CDS             14934..15872
                     /locus_tag="VGA59_16065"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEX9721235.1"
                     /translation="MKAKAMSQFEIVAREDFSEVTYLLEVRHPMMAKAAKPGQFVIVM
                     LNEQGERIPLTIADYDRQRGTITLVIQAVGKTTRQMQQECKVGTFLYGMVGPMGIPSP
                     ISHAKKVLCVGGGLGVAPVFPQARGFKEAGAYVIGILGFRNKDLVFWEDKFRDACDEL
                     ILCTNDGSAGMKGLITDGIAVALEGHRDIDEVVAIGPPVMMKACAEATRARKIKTMVS
                     VNPIMVDGTGMCGGCRVKVGSEVKFACVDGPDFDGHLVDFDDLMSRLRRYTDVEKAAH
                     ERWSKTCRMEQPPAMGGRAKDDVLELPDPSEESLGG"
     gene            15949..17448
                     /gene="gltA"
                     /locus_tag="VGA59_16070"
     CDS             15949..17448
                     /gene="gltA"
                     /locus_tag="VGA59_16070"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEX9721236.1"
                     /translation="MAKKKTIRTIPQERTPMREQDAKARAKNFEEVACGYNLEEALRE
                     SERCLLCPDQPCISGCPVGIDIPGFIQKIGERNLRGAYDVLTDTNLLPSICGRVCPQE
                     NQCEGVCTVGESLEAVAIGRLERFVGDTAIREGWTNVPYIQPTRFRVGIVGSGPAGMA
                     CAADMAKAGCDVTVYEAFHEPGGVLKYGIPDFRLPNTVIDAEIDKLRQLGVKFECNTL
                     VGRLFTIEQMIDEMGYHAVFVGVGAGYPTMLGIPGDSLNGVLSANELLTRCNLMRARD
                     FPAYDTPPSIGKRVAVVGAGNTAMDAMRVSLRLGAEKVYCIYRRSRTEAPARVEEVHH
                     AEQEGVEFHWLTNPVEVLGDGSGSVRGMRCVRMELGEPDDSGRRRPVPVAGSEFDFET
                     DLIVYAIGTNANPIMGQTSKLKLNKWGYIAADDSLATSVAGVFAGGDIVTGAATVIQA
                     MGAGRKAAASMKAYLGIRDPLLGYRPAEGADTFFGIDARERNFARVRVA"
     gene            17514..19442
                     /locus_tag="VGA59_16075"
     CDS             17514..19442
                     /locus_tag="VGA59_16075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX9721237.1"
                     /translation="MDGMTLQQPVDQAGKTAAVRGSAKTPIRLKEHIVEIISDSGEGA
                     QKCGQSLGSIAAQMGNGIWTTEIIPAEIRPPARSVAGASGNRIRIGSGRITNGGDKTD
                     LVISFNEQVLLGRVRANELKPGCTILLESMWRTHKDPAISASYVETYSRLVAAGYRVH
                     EVPMEQACRSLSSMDPKRGKNMFALGMLCQLYSLDLKLAADQIVIAFGKKDRSVVDNN
                     IKLLEGGAVWAKEHLDFCFTIPAVRSTEPQVVVNGNTALALGVLASGMEICAMYPITP
                     ATSASHYLAEVFEKVGGVVHQAEDEIAACAFAVGASYAGKCAITITSGPGYSLKQEAI
                     GLAVMGEIPLVVVNVQRGGPSTGQPTKMEQGDLMTAIFGSHGDAPKIVLAPGTIEECF
                     YSVITARRIAETFNMVVVILTDAGLATSQQPFPRPVFNESWMAPPVDQSPVPEGAKPY
                     AWDETTGLSRRFKPGQPGGMHTLTGLAHDSDSHVAYDAQVNEQTLRFRSLKLAAFQKT
                     LKPPTIFGGPKGDLLIIGWGSTKGAIEEAVEGLRADGKKVSSMHLQFLQPMAPGIKEA
                     MSGFKQVMTIESNWSDRPDDKIIDETNRRYSSLAILLRSRYLVDVDCFSEVCGQPIKP
                     STIRRVAAEKLKKNGKGA"
     gene            19452..20432
                     /locus_tag="VGA59_16080"
     CDS             19452..20432
                     /locus_tag="VGA59_16080"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9721238.1"
                     /translation="MMSPTECLLQMCDEHFSLEDYQSGVPRWCTGCGDNAILAAVQRL
                     CRDEALRPEKTVFVSGIGCSSRFPHYMKTYGFHGIHGRALPVAEGVKMSRPDLTVFVN
                     TGDGDCCSIGAAHWIHAIRYNMNLTVFLHDNQIYGLTKKQASPTSPVGTKSNTTPRGS
                     YLEALNPLAVTLGVQNVSFVAQAVDWIPESLFEIVKAAYHHKGLSFVRIIQRCPEWLP
                     TALDPWMNDPNRVQILHHADGLQPSAGLSKIYKNQLEHDPGNIDRAREIAGQPDDIPV
                     GILYRNPAVPCYEDLRRSDRLVTPEWTRSGLNAELDKYTVWPQEASRKAA"
BASE COUNT         3654 a         6745 c         6887 g         3295 t
ORIGIN      
        1 agaagcgcaa acggcccgaa agtttcaaac cactgcagga ccggttcggg aacccggcgt
       61 ccgtcacgac cgaaaagaag ttcgggggcg gcaatgagta gcgaaggctt cctgggccgc
      121 tggtcgaagc gcaagctcga ggtgaaaaag gcaaaggcgg tggaggcgga gccgacgccg
      181 gagctgccgc ccgcacccca gccctctccc gcgagtggga gagggagcca aaccggatcc
      241 cagtcccgtc tcgccagcgg aagagggagc gaagcagcga taaccgaagc tgcaactgga
      301 cttgctccct ctccccccgg gggagagctc gcgagggggc cgggtggggg tgagggcccg
      361 gtgcaagcgc ccctgccgcc gaccatcgaa gacgtcgaag ccctcacgcc cgagtcggat
      421 ttcacgcgtt tcgccgcccc cgacgtgacg cccgaggtga agaacgcggc cctgaagaaa
      481 ctgttcgccg acccgcgcta caacgtgatg gacggcatgg acgtgtacac cggcgactac
      541 tccaagcccg atccgattcc gcaagcgatg ctgcgccagt tggccggcgc caaattcctg
      601 ggcctgttcc gcgaagagga aaggcaggaa aaagcggcgc gcgacgcaag ggatgatgcg
      661 gataaccaca cgcaccaaaa ggtggcacag tccgggtcgg agtcccaagc agtacccgag
      721 cctcccgaac atgccgaccc tgatctgcaa ttgcaacaag accatgccgc tccaggagaa
      781 gaccctgggc gcggcactgg gtgaggagct gatcctccac accacgctct gccggcgcga
      841 ggccggcgcg ttccagcagg ccgtcaagag cggcgcggac gtcgttgtcg cctgcacgca
      901 ggagcagcgg ctgttcgccg aagtcgggca ggaaacagca ggggcggtgt cgccggtcaa
      961 gttcgtcaac atccgcgaga ccggcggctg gagccgcgac gcatcccgcg ccatgccgaa
     1021 gattgccgcc ttgctggcgg cggcgcacct gcccgacccc gagccggtgg cgacggtgag
     1081 ctacaagagc gcgggcaagc tgttggtcat cgggccgatg gagcaagccg agcgcgcggc
     1141 ggctttcgtc agcgacgcac tggatgtgac cgtgttcacc cagggtggca cgggcggcca
     1201 ggagcggcgc ttcccggtcc tgggcgggcg catcgaaagc ctcaagggct ggctgggggc
     1261 gttcgagctc cagtggagcc gcaacaatcc catcgatctc gatctgtgca cccgttgcaa
     1321 cgcctgcgtc gcggcctgcc ccgagggcgc gatcggcctg gactaccaga tcgacctggc
     1381 caagtgcgca tcgcatcgcg aatgcgtgaa ggtgtgcgag gccgcgggtg ccatcgactt
     1441 cagccgcgag agcgaaaccg acaccgaaaa gttcgacctg gtgctggacc tgcgcgccgc
     1501 gccggcattt ctgcagcatg cgccgccgca aggctacttc cacctgccgg cctcacaagg
     1561 cgacatgctc ggggcagtgc tcaagctgcg cgagctggtc ggcgagttcg agaagcccag
     1621 gttcttcggc tacaagcaaa agctctgcgc ccactcgcgc aacgagaccg tcggctgcaa
     1681 tgcctgcatc gacatctgct cggccgaagc gattctcagc gacaagagcc ggcaacagat
     1741 caaggtcaac ccgaatcttt gtgtcggctg cggcgcctgc actaccgttt gccccaccgg
     1801 cgcgctgact ttcgcctacc cgcgcgcgag cgagcagggg ctcaagttca agacactgct
     1861 ttccacgttc aggcaggccg gtggcaagga tgcggtgctg ctgctgcaca gccaggaacg
     1921 cggccaggcg ctggtggagg aggtcggccg cgctgcccag ctgggtgtcg cccacggcgt
     1981 gcccgccaac gtgatcccgg tggcgctctg gcacacggcc agcctgggcc tggacgtctg
     2041 gctcagtgcc atcgccttcg gtgcttcgcg catcgccgtc ctcctcacgg atgaagaggc
     2101 gctgcagtac cgggaggcct tgcgcgagca gatggaggtc ggtgaggcca tcctgaacgg
     2161 cctgggctac ggcggaagcc acttcaaact gatgcacgcg caccggctcg cggacctcga
     2221 ccaggagctt caattgctgc tggcacagcc gccggtagtg cctgccgcag ccgcgcgctt
     2281 tgcggtggcc ccggaaaagc gcgccacgct ggagctggcg ctggaccatc tggtcgaaaa
     2341 tgcgccggcg cggcccgaag cgatagaact gccggcagcg ggcgccgcgt tcgggacgct
     2401 cgaggtgtcg aaggcaaagt gcaccttgtg cctgagctgc gtcagcgctt gcccggccag
     2461 cgctttgcag gacaaccccg agctgcccca gcttcgtttc atcgagaaaa actgcgtcca
     2521 atgcggcctg tgcgccacca cctgcccgga ggacgcgatc gtgctcaggc cgcgcctgct
     2581 gctcacgcgc gaacgcaatg aagcacgtgt gctcaacgaa acccggccgt acgcctgcgt
     2641 gcgctgcggc aagccgttcg gcacgctcaa ggccatcgag gcgatgctcg gcaagctggc
     2701 cggccattcg atgttccagg gcgaggcgct ggagcggttg aagatgtgcg ccgactgccg
     2761 agtgatcgac atgtactcgg cccgcaacga agtgaagatc acggatctct gaatgcatcc
     2821 caacacgtcg gaacggattc ccgtgtcatc cgccttggac gaggagaccg cgcgagccga
     2881 ggtctacggc ctgctggccc agctctacta cgcgagcgca accccggaac tgttgagtgc
     2941 gatccgggcg gccgtcaccg aggcgcccgc cgccggtgcc ttcctcgagg agccgtggcg
     3001 cgaagtggtc ggccttgcgc gcagcatgga cgacgccgcg gtcgcagcgg aatacgacag
     3061 cctgttcggc ggcgtcggca agcccgaggt ctaccttttc ggctcccatt acctcagcgg
     3121 cttcctgaac gacaggccgg tcgcccggct gcgttccgat ctggccgccc tggggctcga
     3181 gcgcgacggg accgtgaccg agaccgagga ccatatcgcc tgcctgtgcg aggtcatgcg
     3241 ctacctgatc gcgggtgacg acgtggcggt ggccaatctg acgcgccagc gcgagttctt
     3301 cgccacccac cttcaaccct gggtgcccac cttgtgcgac acgatcgccc ggcatcccaa
     3361 ggcgcgcttc tatgcaacgc tcgccggttt cacgcaggct ttcatcggcg tcgagatcca
     3421 gggtttcgac atgctcgact gatgagctgg gcgcacgccc tcggtgcgtg tgcaaccttt
     3481 cgactcaagt acttgccccg tgtttacatg cttgcatcgg gcggtacatt cgattcgtaa
     3541 aagtcgttgg gcctcccctg gggaggcaat taggagacaa gccatgagcc agccaacagg
     3601 caagctaagc cgcaggcatc tttttgtggg tgcgggaacc gcgggagccg tcgccgcggc
     3661 agccagcctc gtgcccggcg cggtgcagga aacggtcgca accccacaag ccaaggagcc
     3721 gcccgcgcgg ggcggcggct accagctgac cgaacacgtc aagctgtatt acaagaccac
     3781 cctcgtttaa ctctcaccac caggagagtc agccatgttg ttgaccagga agatgggcgc
     3841 tgcccaggcc gccgcgcgtt ccccattcgt tcacagtctg cagcgaggcc tcgcgcgcgc
     3901 actgcccacc atggaccggc gtgccttcct gcgccgctcg ggccttggtg ttggcgtggg
     3961 catcgcagcg tcctcactca cgctgatcaa gaaggccaag gcggccgagg gcaaggcgac
     4021 ctcggtaggg cagggcaaga tcgaggtgaa gcgcaccgtg tgcacccact gctccgtcgg
     4081 ctgcgccatc gacgcggtgg tcgaaaacgg cgtgtgggtg cgccaggagc cggtgttcga
     4141 ctcgcccctg aacctgggcg cgcattgcgc caagggcgcg gcgatccgcg aacacggcca
     4201 cggcgaattc aggctgcgct atccgatgaa gctggtgaac ggcaagtacg agcgcatcac
     4261 ctgggatacg gcactgaacg agatcagcgc gaagatcctg gacctgcgca aagccagcgg
     4321 cccggattcg atttactgga tcggctcgtc caagcacagt aacgagcagt cctacctgat
     4381 gcgcaagttc gtcagcctgt ggggcagcaa caactgcgac caccaggccc gcatctgcca
     4441 ttccaccacg gtggccggcg tcgccaacac atggggctat ggcgccatga ccaattcata
     4501 caacgacatg cagaacagca agtgcgcgtt gtatatcggc tcgaacgcgg cggaggccca
     4561 tccggtgagc atgctgcaca tgctgcacgc gaaggaaacc ggcaccaaga tgatcgtggt
     4621 ggatccgcgt ttcacgcgca cggccgccaa ggcggacgaa tacgtgcgca tacgttcggg
     4681 ctcggacatc ccgttcctgt tcggcttatt gcaccacatc ttcaagaacg gctgggaaga
     4741 ccagaagtac atcaacgacc gtgtctacgg catggacaag gtcaaggccg acgtgatggc
     4801 caaatggact cccgacaagg tcgaggaagc ctgcggcgtg cccgaagccc agatggccaa
     4861 ggtcgcggag atgatggcca agaaccggcc gtcgaccatc gtctggtgca tgggccagac
     4921 gcagcacacg atcggcaatg ccatggtgcg ggcctcgtgc atcctgcaac tggcgctcgg
     4981 caacatcggc gtgtccggcg gcggcgcgaa catcttccgc ggccatgaca acgtgcaggg
     5041 cgcgaccgac gtgggtccca atcccgattc gctgcccggc tactacggca tcgccgaagg
     5101 ctcgtggaag cacttcgcca aagtctgggg cgtggacttc gactggatca aggcacgctt
     5161 cgccaatccc ggcatgatga gcaagccggg catcacggta tcgcgctgga tcgacggcgt
     5221 gatggagaag aacgagctga tcgaccagga tccgaacctg cgcggcgtgg tgttctgggg
     5281 ccatgcgccc aactcgcaat cccgcggcct ggaaatgaag cgggccatgg acaagctgga
     5341 tctgctggtg gtggtggacc cctatccatc ggctacggcg gcgatggcgg cgatgcccgg
     5401 caagcccgag gatctcaacc ccaaccgggc cgtgtacttg ctgccggccg cgacgcagtt
     5461 cgaaaccagc ggttccgtca cggcgtccaa ccgttcgctg caatggcgcg agaaggtcat
     5521 cgagccgcta tgggaaagcc gcagcgacca gatgatcatg caccagctgg ccgagaagct
     5581 cggcttcggc aaggagctgt cccagaactt caagatgcag aaagtcaagg gcatggacga
     5641 gccggtgccc gaggagatcc tgcgcgagat caacaggagc gtgtggacca tcggctacac
     5701 gggccagagc cccgaacgcc tgaaggcgca tatgcgcaac atggccaact tcgacgtgaa
     5761 gacgctcaag tcgaagggcg gcaaggacaa ggagaccggc tacgacctca ccggcgacta
     5821 tttcggcctg ccgtggccaa gttacgggaa ccccgaactc aagcacccgg gctcccccaa
     5881 cctgtacgac acctccaagc acgtgatgga cggcggcggc aacttccgcg cgaacttcgg
     5941 cgtggaaaag gacggcgtca acctgctggc cgaggatggc tcggcctcca tgggcgcgga
     6001 catccagacc ggttatcccg agttcgacca cgtgctgctc aagaaactgg gctggtggaa
     6061 cgagctcacg gacgtggaaa aggccgcggc cgaaggcaag aactggaaga ccgactcctc
     6121 gggcggcatc atccgcgtga ccatgaagaa ccatggctgc cacgtgttcg gcaatgccaa
     6181 ggcgcgggcc gtggtgtgga actttccgga tgggatcccg cagcaccgcg aggcgctcta
     6241 cagcacgcgg cccgacctgg tggccaagta cccgactcac gacgacaaga aggccttctg
     6301 gcgcctgccc acgctctaca agaccttgca gcagcagaac atcaccgata aggtcgcgga
     6361 aaagtatccg ctgatcctca cttcgggccg gctgaccgag tacgagggtg gcggcgagga
     6421 aacgcggtcc aacccgtggc tggccgagtt gcagcaggag gcgttcgtcg aaatcaaccc
     6481 gaaggccgcc gccgagcggg gcatccgcaa cggcgaccgc gtgtggctga tgacacccac
     6541 gggcgcgcgc ctgaacgtgc aggccctggt gacggaacgc gtgggccccg acacctgctg
     6601 gatgccgttc cacttctccg gccgctggca aggcgtggac atgctgccct attacccgag
     6661 cggcgcggcg ccggtcgtgc gtggcgaagc ggtgaatacc gccacgacct acggctacga
     6721 cagcgtgacc atgatgcagg aaaccaagac gacgatctgc aacgtcgaaa aggcggcatg
     6781 aaaaaggttc tgtcatcccg gactcgatcc gggatccatg ctggcgtgga ttgcgggtcg
     6841 agcccgcaat gacaagccga aaaaggagct gacatggcac gaatgaaatt catttgcgat
     6901 tccgagcgct gcatcgaatg caacggctgc gtcacggcgt gcaagaacga gcatgaggtg
     6961 ccctggggcg tgaaccgccg gcgggtggtc acgctcaacg acggcatccc cggcgagcgt
     7021 tcgatctcgg tggcgtgcat gcattgctcc gacgcgcctt gcatggccgt gtgcccggtg
     7081 aactgcttct accgcaccga cgaaggcgtg gtgctgcacg acaaggacgt ctgcatcggc
     7141 tgcggctact gcagctacgc ctgtccgttc ggcgcgccgc agtttccggc ggcgggcacc
     7201 ttcggcgtgc gcggcaagat ggacaagtgc acgttctgcg ccggcggtcc ggaagcccac
     7261 ggctcgcagg ccgaattcga caagtacggg cgcaaccggc tggccgaagg caagcttccc
     7321 gcctgcgccg agatgtgctc gaccaaggcg ctgatcggcg gcgacggcga cgtggtcgcg
     7381 gatatcttcc gcaaccgcgt gatcaagcgc gggaaggggg ccgaagcctg gggctggggc
     7441 acggcctacg gttcgaggca ggccggccag cccgccggag gaaaatcatg aagcgcaaac
     7501 tgatccttgc ggccgccgtg gtggcactgg ctgcctgcac tgacaagccc cagaccctcg
     7561 aaagtggcgt ccgggccact gccgccgggc cggacagcgc cgcctttcag ggcaccggca
     7621 tgccgttcgc ggcgccgggc tggaagccgg gcgacaaggt cagctgggag cagcacctga
     7681 agacacgcac ccagaacggc cagaacgact acaccagggt gaattgaaag gccgatgatg
     7741 ctgcgttcat tgctgctgac tttcgcgctg gccgcgagct tgcctgcttt tgcgcagacg
     7801 aaggcgccgg acgacccggc gccgggtgcg acggcaccgg ccgcgccggg gggcatccag
     7861 agccagaaca tcttcgacgt caagcccgag gtcaagcctg acgcgagttc cgatcctgac
     7921 tacatgaagc agaacaatgc cgagcgcaac agggtgcagc ccggcaacaa cgcgccgatc
     7981 tggcgcggtg tcgtctcggg gatggaaggc ttcaccagcc tgcccaagag ccaggcgccc
     8041 gaggccggtg tcctgatcca ggcgcccgtg caatacccgg gctcgcgcat gaccacggcc
     8101 ggggaagcct ggcggcaggt gcgcaacaac tggatcatcc cgtacggcgg cgcactgttc
     8161 ctgatcggtc tgggtgcgtt ggcgatcttc tacttcgccc gcgggccgct gggcggggat
     8221 gccgaatccg gccgcaagat cgaacgcttc acaccgttcg agcgttcggc gcactgggcc
     8281 aacgcgatcg cgttctgcat cctggccatt tcgggcctgg tgatggcgtt cggcaagttc
     8341 ttcctcctgc ccgtcatggg cggcacgctg ttcggctggc tcacgtacgt gctcaagaac
     8401 ctgcacaact ttgccgggcc ggttttcgcg gtgtcgctcg tcatcgtgat cgtaaccttc
     8461 atgcgcgatg aatggccgca gcgtggcgac ctgaggtggc tgctcaggat cggcggtgca
     8521 ttcgacaaga atggtcatca gccgcccgca gccaaccgct tcaacgccgg cgaaaaggtg
     8581 atcttctggt tcggcgtgtt cctgctgggc atcattgttg tcggctcggg cctggtgatg
     8641 gatatgctca tcccgaacgt ggtgtacgag cgcggcacga tgcaggtcac caacatgatc
     8701 cacggggtgg ccgccgtgtt gatgatgacg ctcttcctgc tccacatcta cctgggcacg
     8761 atcggcatgc gcggcgcgta catgggcatg cgtaccggct atgtggacga ggcgtgggcg
     8821 agggagcacc actcgtactg gtacgaagat atcaaggccg gcaagatccc ggtgcagcgc
     8881 agccagccga cggcgatcgc cgatggcgcg gaaaccgcgc gaccggcctg aagcgacagc
     8941 aggagagaaa ctcatgaaaa cacttctggt atcgactgtg ttggcgaccg cgtgcgccct
     9001 ggcgtgggcc aagctgccac cgccggacga tgcggccaag gccaaggcgg ccgaaacggc
     9061 ggccaagtcg gcctggcagg gcaaggtcga tgcccacctg ctgtgcaagg cgcaggacaa
     9121 ggtggcggcg tattacatga agaccgccgc caagaagccc gcaccggccg ccgccccatc
     9181 agctgccgcc gctcccgctg ctgccgctcc tgctgctacc gcttcggctg ccgccgcgcc
     9241 ggcccagccc gcggcttccg gcacgcccgt agcggcgaag ccgccgccgc cttgtgccga
     9301 tccagggccg ttcgcctaca acccgccgga gcagaaaccc cttgaaaccg caggtgcgca
     9361 ttcgcccagc ggtacggctg ccagcccgcc cagcgtaaag ccgccttcgg ccgagatggc
     9421 gcccgccaag aagtaaggcc cccaaccgac ccgcgtcggt ttggacaggg cgaggaatgg
     9481 ccgcgtctcg cggccatgcg gcctgcaagg cccaccagcg gcccggcaaa gccggttccg
     9541 cggtggcacg cgaattgacc cgtcaggcaa cggctaagcg gttttgaggt agcctagagc
     9601 gatgcccctg cctttcctca ccgaagcacg cgcaccgctg acgcaggaaa tcgcgaccgt
     9661 caacgagtat ggcgagcggg acacggtttc ggtccccgcc gaacggccgc tgaccgtcta
     9721 tgtggacaag cgcgaactgg tcaccttgat gaccttgggc gcgcggccgg aatggctggt
     9781 gctgggctat ctccgaaacc agcggctggt gaacgcgctg gacgaaatcg agtcgatcac
     9841 ggtcgactgg gaggtcggcg cggccgcggt taacacccgt tgcgggatag agcggatcga
     9901 agagcgcacg gcgaagcgcg tggtcacgac cggttgcggg cagggcagtg tttttggcgg
     9961 cctcatggat gagatcgaat cgatcagctt gccgcaggcc tcgatcacgc agtcccagct
    10021 ctacggcatc gtcaatgcga tccggctaca ggagagcaca tacaagtcgg ccggctccgt
    10081 ccacggctgc gcgctgtttc gtggcgaggg gatgctgctg ttcgtggaag acgtggggcg
    10141 ccacaacgcc atcgatacca tcgccggctg gatgtggctg caggatccac ccatggaaag
    10201 caacgacaag gttttttaca cgaccggccg gctgaccagc gaaatggtca tgaagtccgc
    10261 gcagatgggc gttcccatcg tgatttcccg cagcggcatc acgcagatgg gccacgcgat
    10321 cgcccagcgc ctgagcctgt gcgccatcgg gcgggccacc aacaagcgct ttctctgcta
    10381 cagcgcgccg cagcgcctgc aattacagcc ggaactggcc gggcccaagt tgcgcgcggc
    10441 cgcctgattt cccaggcgct gaagtgaaag cccacccccg aagcgccttc ggcgcctccc
    10501 cctcgagggg gcgccaccag cggaccggca aagccggttc cgcggtggcc cccgcactga
    10561 tcgcacgatt tcatacgctg caggtggcgc gcagcgcgtt ggagcaactg gcatgatgcc
    10621 gcccaccgac gatgcgccgt ttccgacgcc ggcaactgcc gctgccccgg ctgccccgga
    10681 gcgcctgccg ctgcgcgtct ggctgcgcga aggcctgcgc gccgggttct tcctgccccc
    10741 gcgcatcgcc ggcgagcagc cgacgccgct gcaggtcgcg gccttgctgc tcatcttctc
    10801 gctgctggaa atcggcctcg cgcggctcga ggtggccggt gccgcgcact tcgacctgcg
    10861 cggctggctg gccccctggt ggagcaccgc ggcgctgctc ttgctggcat ggtgggttct
    10921 gccgcccata gcgccgggcg cgccgggccg cccggccggt ctggcggcgt ggttcgtgct
    10981 ttggctgtcg gctgtgcttc cggtcaatac ggtctcgcag ctgctgggaa tcgcgcaggc
    11041 ccacgagatg ctgccgcaac agctcgagac ctcggcctgg gccgcctggg tcctgtacct
    11101 gctgctatgg gcctggaccg tgggggtggt actgcgcatg acggcgtggt tcggcgctgg
    11161 gctgccgcgg ctgggcgtgc tggcgttggg aatggtgggt ctgttcggcc tggccgccgt
    11221 gcagttccct gaccgcccct ggcaagccga ggcgcccggc gacaagcgac cgcggctgga
    11281 gctgagccag gaactgttcg aagcgcagcg ggcgctctgg caagaaacga tgtccggcat
    11341 cgcgcccgac cgtgccggca ccgtcgacgt ctacgcactg gtcttcgcgc cctatgccgg
    11401 cgaggatgtg ttcctgcgcg aaagcggcat ggtcgccaag ctgttgacgg agcggttcga
    11461 tgccgggggg cgggtcgtcc acctggccaa tcatccctta accgcgcgca cgattccctg
    11521 ggcgacgcag ctgaacctcg agcgggccgt cgaggcactg gcggcgcgga tggaccgcga
    11581 gcacgacgtg ctggtggtgt acctgacctc gcacggcgcc gcggacttca agctggccgc
    11641 cgctcacggg cctttggagg tgaaaccggt cggcccggcg gacttgcgcc gggcgctcga
    11701 ccgggccggg atccgcaatc gggtgatcgc cgtgtcggcc tgttactccg gcggttgggt
    11761 cggtccgctg gcgagcgaga ccacgctcgt gatgaccgcc gccgatgtcg accgcacatc
    11821 gtacggatgc ggccggcggt ccgaactgac gttcttcggc cgtgcggtct tcgatgaaca
    11881 gttgcgccag acccattctt tcgagcaggc ctttgccgcg gcggtgccgc tgatccggca
    11941 gcgcgagatc gaagccggca aggaggatgg cttttccaat ccgcagatca gcgtgggcga
    12001 gaagatccgg ccggtgctgc gggacctgga acgccggctc ggtaccgcgg cggcgggcgc
    12061 cggcgctaat cccggcctga gtcttgtgcg gtagggtggc ctcatgaccg cgacttcctc
    12121 ctggcaactg ggctggcgca ccctctggcg cgacctgcgc gccggcgaac tgcgcctgtt
    12181 gatcgtggcc gtcacgctcg ccgtggccgc gctgactgcc gtcgggtttc tggcggaccg
    12241 gctcaagggc ggcttggtgc gcgacgcgcg ccagctgctc ggcggcgacg ccgtgatctc
    12301 cagcgacaac ccgacgccgc aggcctttat cgaccaggcg cgcgcgctcg gccttcgcag
    12361 cctctccacg ctgagtttcc cgaccatggc gcgcgcgccg gatgcgcggg gcggggcgag
    12421 ccggctcgtt gcgctcaagg cggtgccggc tggctatccg ctgcgtggca acctcaaggt
    12481 ggcaccccag ccggaggcgc ccggcgccga tacgcgcgac atacccgccc cgggcgaggc
    12541 ctgggtcgac gctcccttgc tggacatgct gggcctgaag gtgggagatc ccgtgttgct
    12601 gggcgatgcg cagctgcgcg tggcgcgcat catcgtgctc gagccggatc gcggcaccgg
    12661 cttcgtgaac ttcgcgccgc gcgtgatgat caatgaagcc gacctggccg cgacgcggct
    12721 gattcagccg gccagccgcg tcggctaccg cttcgcagtg gccggagcgg atccccgggt
    12781 gagcatgttt gccaggtggg cggcgcagga agtcaagagc ccggcggtgc gcggcgtgcg
    12841 cctggagtcg atggagagcg gccgtccgga gatgcggcaa acgctcgacc gcgccgaaaa
    12901 attcctgaac ctggtggcgt tgctggccgc gctgctgagc gcggtggccg tcgccctcgc
    12961 cgcacgtggt ttcgccggca agcaccttga cgattgcgcc atgttgcggg tgctgggaca
    13021 gagccagcgc accatcgcgc tcagctatac gttcgaattc gccctggtcg gcatcgcggc
    13081 cagtgcactg ggtgtcgcgc tcggctactc ggtccacttc gtcttcctcc agcttctcgc
    13141 cggtctggtc gaagcgacgc tgccggccgc gaccttgtgg ccggtggcgt tcggcatggg
    13201 catggggctg accttgctgt tcgcattcgg attgccgccg gtgttgcagc tggcccaggt
    13261 cccgccgctg cgagtcattc ggcgcgacct cggaaatctc aagccggctt cgctgtcggt
    13321 gctggcgatc ggtgtggccg gcttcgccgc cttgctgctg gcagccagca gcgacctgaa
    13381 gctgggagcc atcgccgtgg gcggcttcgc cggggccgcc ctgctgttcg ccggcctgag
    13441 ctggctcgcg gtgaagctgc tgcgcctgtc ggtcaacgag gccacggcgc cacgctggat
    13501 ggtgctggcg acgcggcaga tttccgcgcg gccggcctat gccgtggtcc aggtcagtgc
    13561 gttggccgtg ggcttgctcg cactggtgct gctggtgttg ctgcgcaccg acctggtcgc
    13621 cagctggcgc cgcgccacgc cgccggacgc gccgaaccgc ttcgtcatca acgtcatgcc
    13681 ggagcagagc gagaccttcc agaagatgct gcggggcgcc ggcgtccagc ggttcgactg
    13741 gtatccgatg atccgcgggc gcctggtcgc ggtgaacggc agggccgtga cgcccgacga
    13801 ctatccggag gaccgggcca agcggctgct ggaccgcgag ttcaaccttt ccaccaccac
    13861 ggtgcagccg ccccacaacc aggtggtggc ggggcgctgg cagagcgaag agcaaggcgc
    13921 cgtgagcgtg gaagagggca tcgcgaatac gctgggactg aagatgggcg acacgctgcg
    13981 cttcgacatc ggcggcatgc aggaggatgc gaagatcacg tcgctgcgca aggtcgactg
    14041 gggttcgatg cgggccaact ttttcgtcat ctatccggtg acccgcttgc agggtgtacc
    14101 ggtgacctac atgagtgctt ttcgcgcgcc cgatcgcaag ggattcgaca acgagctggt
    14161 gcgggcgttc cccaacatca cgaccgttga cacgaccagc accatcaacc aggtgcaggg
    14221 cgtgctggac aaggtgatcc gcgccgtcga gttcctgttc ggcttcacgc tggccgccgg
    14281 cgtcgtggtg ctgttcgccg ccgtgaccgc cacacgcgag gagcgggcgc gcgagttcgc
    14341 gatcatgcgc gccgtggggg ccagcagcgc actgctgcgc caggtgcagc gcgcggaact
    14401 cgtgggcgtg ggcctgctcg ccggctttct cgcgagcgtc gtggcgtcgg ccgtaggctg
    14461 gggattggcg cgattcgttt tcgatttcga ctggacagcg tcggcgtggg taccgctggt
    14521 cggcgcgctg gcgggcgcgg cgctggcgct cgcggccggc tggtggggcc tgcgcgaagt
    14581 cctcaaccgg ccggtcatcg aaacattgcg gcgtgcgccg gagtagcacg ctgcgcgtca
    14641 gcttcgcgta atattcacgc ctcgtttgtt catcccctca tcaagacgcc gatgggtgcg
    14701 taacgtgctt atataagagg caatcggcgc cgcgctttca ctcgctcgat acacgctgcc
    14761 gacggttgcg gggctacttg acttgcatca actgccccat gcgccgcgcg gaagatactt
    14821 gcccgcatcc aatcggtggc gcttcggaga gcccttgaag ggcctggtac accgatcggc
    14881 gccaagagga gttgcagcct gaccggcccc gaagcccgtc agtggcggcc cacatgaaag
    14941 cgaaagctat gtcccagttc gagatcgtgg cacgggagga tttctccgag gtcacctatc
    15001 tgctcgaggt acgtcatccg atgatggcga aggccgcgaa acccgggcaa ttcgtcatcg
    15061 tgatgctcaa tgagcaaggc gagcgcattc cgctcaccat cgccgactac gatcgccaac
    15121 gcggaaccat caccctcgtc atccaggccg tcggcaagac caccaggcag atgcagcagg
    15181 agtgcaaggt cggcaccttc ctgtacggca tggtcggccc gatgggtatt cccagcccga
    15241 tcagccacgc caagaaggtg ctgtgcgtcg gcggcggcct cggcgttgcg ccggtgttcc
    15301 cgcaggcgcg gggcttcaag gaggccggcg cctacgtgat cggcatcctg gggtttcgca
    15361 acaaggacct ggtgttctgg gaagacaagt tccgcgacgc gtgcgacgag ctcatcctgt
    15421 gcaccaacga tggttccgcc gggatgaagg ggctcatcac cgatggcatc gccgtcgcgc
    15481 tcgaaggcca tcgcgacatc gacgaggtgg ttgccatcgg cccgcccgtg atgatgaagg
    15541 cttgcgccga agcgacccgt gcgcgaaaga tcaagacgat ggtcagcgtc aatccgatca
    15601 tggtcgacgg cacgggcatg tgcggtggct gccgggtcaa ggtgggcagc gaggtgaagt
    15661 tcgcctgtgt cgacggcccg gacttcgacg gccacctggt cgatttcgac gacctgatgt
    15721 cgcggttgcg ccgctacacc gatgtcgaaa aggcggcgca tgagcgctgg tccaagactt
    15781 gccgcatgga acagccgccg gccatgggtg gccgcgcgaa agacgatgtg ctcgagctgc
    15841 ccgatccctc cgaggagtcg cttggtggct agatacctgc cgattttttc atcgatgcgc
    15901 gccgtgggta tccggcgcgc ctccaagtca gagtgagagg tgtaaggaat ggccaagaag
    15961 aaaacgatcc gaaccattcc ccaggagcgc acgccgatgc gcgagcagga cgccaaggcg
    16021 agggcgaaga acttcgagga ggtcgcatgc ggctacaacc tcgaagaggc tttgcgcgaa
    16081 tcggagcgtt gcctgctgtg tcccgaccag ccctgtatct cgggctgtcc cgtcggcatc
    16141 gacatcccgg gtttcatcca gaagatcggt gagcgcaacc tgcgcggcgc gtacgacgtc
    16201 ctcacggaca ccaacctgct gccctcgatc tgcgggcggg tctgcccgca ggagaaccag
    16261 tgcgaaggcg tctgcacggt cggggaatcg ctggaagcgg tggcgatcgg aaggctggag
    16321 cggttcgtcg gcgacaccgc catccgcgaa ggctggacca acgtccccta tatccagccc
    16381 acgcgttttc gcgtcggcat cgtcggctcg gggcccgcgg gcatggcctg cgccgcggac
    16441 atggccaagg ccggttgcga cgtcacggtc tatgaagcgt tccatgagcc cggcggtgtg
    16501 ctgaagtacg gcatccccga cttccggctt ccgaacacgg tgatcgacgc cgagatcgac
    16561 aagctgcggc agctgggcgt caagttcgag tgcaacacgc tcgtgggccg cttgttcacc
    16621 atcgagcaga tgatcgacga gatgggctac cacgcggtgt tcgtcggcgt cggcgcgggc
    16681 taccccacga tgctgggcat cccgggcgac tccctcaacg gcgtgctgtc ggccaatgaa
    16741 ttgctcaccc gctgcaacct gatgcgcgcg cgcgactttc ccgcttacga cacgccgcct
    16801 tccatcggca agcgcgtcgc cgtggtgggc gccggcaaca cggcgatgga cgccatgcgc
    16861 gtttcgctgc ggctgggcgc cgaaaaggtg tactgcatct accggcgttc gcgcacggag
    16921 gcgccggcgc gtgtcgagga agtgcaccac gccgaacagg aaggcgtcga attccactgg
    16981 ctcaccaatc ccgtcgaggt gctcggcgat ggctcggggt ccgtgcgcgg catgcgctgt
    17041 gtccgcatgg aactcggcga gcccgatgac tccggccgca ggcgtccggt gcccgtggcc
    17101 ggcagcgaat tcgatttcga gaccgacctg atcgtctacg cgatcggcac caatgccaat
    17161 ccgatcatgg gccagacctc gaagctcaag ctcaacaagt ggggctacat cgccgctgac
    17221 gacagcctcg ccacttcggt ggccggtgtg ttcgcgggcg gcgacatcgt cacgggcgcg
    17281 gcgacggtca tccaggcgat gggcgccggg cgcaaggccg ccgccagcat gaaggcctat
    17341 ctcggcatcc gcgatccctt gctggggtat cgcccggccg agggggcgga cacatttttc
    17401 ggcatcgatg cgagagagcg caatttcgcg cgcgtgcggg tggcctagtc gcgcgggttt
    17461 gtgacctgcg aagctgccgc gccaaacgtt tgaacaaggg ctacggaaac accatggacg
    17521 gaatgaccct gcaacaaccg gtcgaccagg ccggcaagac cgccgcggta cgcggcagcg
    17581 ccaagacgcc gatccggctg aaagagcaca tcgtcgaaat catcagcgac tccggtgagg
    17641 gcgcgcagaa atgcggccag tccctcggct cgatcgccgc ccagatgggc aacggcatct
    17701 ggaccaccga gatcatcccc gctgaaattc gcccgccggc gcgcagcgtg gccggcgcga
    17761 gcggcaaccg catccgcatc ggatcgggcc gtatcacgaa cggcggcgac aagaccgacc
    17821 tcgttatttc cttcaacgaa caggtgctgc tcgggcgcgt gcgtgccaat gagctcaagc
    17881 ccggctgcac catcctgctc gagagcatgt ggcgcaccca caaggacccc gccatctcgg
    17941 cctcctatgt cgagacctat agccgcctcg tggcggccgg ctaccgggtc catgaagtac
    18001 cgatggagca ggcgtgccgc tcgctgagct cgatggatcc caagcgcggc aagaacatgt
    18061 tcgcgctcgg catgctgtgc cagctgtaca gcctggacct gaagctcgcc gccgaccaga
    18121 tcgtgatcgc cttcggcaag aaggaccgca gcgtggtgga caacaacatc aagctgctcg
    18181 aggggggcgc cgtctgggcc aaggagcatc tcgacttctg cttcaccatt cccgccgtgc
    18241 gctcgacgga accgcaggtc gtcgtcaacg gcaacacggc gctggccctg ggcgtgctcg
    18301 cctcgggcat ggaaatctgc gcgatgtatc cgatcacacc ggctacttcc gcctcccact
    18361 atctggccga ggtcttcgag aaggtcggcg gcgtggtcca ccaggccgaa gacgagatcg
    18421 ccgcatgcgc cttcgcggtc ggcgcgtcct acgccggcaa gtgcgcgatc accatcacgt
    18481 ccgggccggg ctactccctc aagcaggagg ccatcggcct ggcggtgatg ggcgagatcc
    18541 cgctggtcgt cgtcaacgtg cagcgcggcg ggcccagcac cggccagccc accaagatgg
    18601 agcagggcga cctgatgacg gccatcttcg gcagccacgg cgacgcgccc aagatcgtgc
    18661 tggctccggg caccatcgag gagtgtttct actccgtcat caccgcgcgc cggatcgccg
    18721 aaaccttcaa catggtggtc gtcatcctga cggacgccgg cctcgccacc tcgcagcagc
    18781 cgttcccgcg gccggtgttc aacgagagct ggatggcgcc gccggtcgac cagagccccg
    18841 tgcccgaggg cgccaagccc tacgcctggg acgaaaccac gggcctgtcc cgccggttca
    18901 agcccgggca accgggcggc atgcacacgc tcaccggcct ggcccacgac agcgacagcc
    18961 acgtggccta cgacgcgcag gtcaatgagc agacgctgcg cttccgcagc ctcaagctgg
    19021 ccgcattcca gaaaacgctg aagccgccga cgatcttcgg cggtcccaag ggcgacctgc
    19081 tgatcatcgg ctggggcagc accaagggcg ccatcgagga agccgtcgaa ggcctgcgcg
    19141 ccgacggcaa gaaggtttcg tcgatgcacc tgcagtttct gcagcccatg gcccccggca
    19201 tcaaggaggc gatgagcggc ttcaagcagg tgatgaccat cgagagcaac tggagcgatc
    19261 gccccgacga caagatcatc gacgagacca accgccgcta ttcgtcgctg gcgattctgc
    19321 tgcgctcgcg ctacctggtc gatgtggact gcttcagcga agtctgcggc cagcccatca
    19381 agccctccac gatccgccgt gtggcggccg agaaactcaa gaagaacgga aagggggctt
    19441 gagccatgga catgatgtcg cccacggaat gcctgctgca gatgtgcgat gagcacttct
    19501 cgctggaaga ctatcaaagc ggcgtgccgc gctggtgcac cggctgcggt gacaacgcga
    19561 tcctcgcggc ggtgcagcgg ctgtgccggg acgaggccct gcgccccgag aaaacggtgt
    19621 tcgtgtcggg catcggctgt tccagccgtt tcccgcacta catgaagacc tacggcttcc
    19681 acggcatcca tggccgcgcg ctgcccgtgg cagagggtgt gaagatgtcg cggccggacc
    19741 tcaccgtgtt tgtcaacacc ggcgacggcg actgctgcag catcggtgct gcccactgga
    19801 tccatgcgat ccgctacaac atgaacctca cggtgttcct gcacgacaac cagatctacg
    19861 ggttgacgaa gaagcaggca tcgcccacct cgccggtcgg cacgaagagc aacaccacgc
    19921 ccaggggcag ctacctcgag gccctgaatc cgctggcggt cacgctgggt gtgcagaacg
    19981 tgtcgttcgt ggcccaggcc gtggactgga ttccggagtc cctgttcgag atcgtcaagg
    20041 ccgcttacca ccacaagggc ctgtcgttcg tgcggatcat ccagcgttgc cccgagtggc
    20101 tgcccacggc gctagacccc tggatgaacg accccaaccg ggtgcagatc ctgcaccacg
    20161 ccgacggcct gcagcccagc gccggcctgt cgaagatcta caagaaccag ctggaacacg
    20221 atccgggcaa catcgaccgc gcacgcgaaa tcgccggcca gcccgacgat atcccggtgg
    20281 gcatccttta tcgcaatccg gccgtgccct gctatgagga cctgcgccgg tccgaccggc
    20341 tcgtcacgcc cgaatggacc cgctcgggcc tgaacgccga actggacaag tacacggtct
    20401 ggccgcaaga ggcgagccgc aaggccgctt gaagcgttgc cccgccgagc cacacagaac
    20461 aggttgaacc cgatggaagc cgatacccaa gcacacgttg catttttcct gaccggccgg
    20521 cggccgggcg aatccctgga cgccgtggaa gggctggacc tgcgtcccgc tctcttcgcc
    20581 g
//
LOCUS       DASWIY010000451         8227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1823251, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000451 DASWIY010000000
VERSION     DASWIY010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823251"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="VGA59_16085"
     CDS             <1..1397
                     /locus_tag="VGA59_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9721239.1"
                     /translation="GFALWAYTLFVPAIVKSGWLPMSTSFINEGPFGIALLKPYALFG
                     LAGLDEISHSLLWTMLANVGCYIGVSLLGRPTVSEQAQAVLFVDVFKMAERGIGLWRG
                     SASMHDIIVLVGRFLGPARAEEAFAGYAARRGLKSAREIEPDAELVRFAENLLAGAIG
                     ASSARVMLASAVKEEALGMDEVFDILDEASQVIAYSRQLEQKSLELEAATAELTAANE
                     SLKELDRLKDNFITTVTHELRTPLTSIRAFSEILHDNPMLEQDQRMEFISIIVKESER
                     LTRLINQVLDLAKLESGNAEWHSTELDMNEVIHEAVSTTSQLLRESRVVLALDLPASV
                     PPVHADRDRLMQVMLNLISNAVKFCDRAAGRIEIALRVLPDALQVDVHDNGPGVAAAH
                     QELIFEKFRQVGDTLTEKPQGTGLGLPISRQIIGHLGGRLWVSSRPGQGATFSFTLPL
                     KPPLRQSPSLDHRP"
     gene            1394..1771
                     /locus_tag="VGA59_16090"
     CDS             1394..1771
                     /locus_tag="VGA59_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9721240.1"
                     /translation="MSKTVLIADDEPNIVISLEFLLEQDGYRVLLARDGHEALEAIER
                     EVPDLVLLDVMLPRLSGYDVCQRIRQKPELAKVRVIMLTARGREVEVTKGLALGADAY
                     VTKPFSTRDLLVQIRRQLGDAPP"
     gene            1796..3925
                     /locus_tag="VGA59_16095"
     CDS             1796..3925
                     /locus_tag="VGA59_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX9721241.1"
                     /translation="MSLRSKYLLWLALSSLLALALVAMVGLAFWSDLQEPERRAVADM
                     LGSRQLLLVMLALLPPVAIGALLRLALAAYPAAAVKLAEEVRLIHSVHPGHRLAPTGA
                     PEMHQLGAAVNELAEAHRALHQEVQARIEEANAHLAQERNRLAALMSELAQSVLVCNV
                     EGRILLYNARASRVLEQADGAEVPVGLGRSIFGILDRSPIIHALGQLGHRLEQGTPQP
                     VARFVTTRGGRLLRAQMAPVLDQYGAITGFVLMLEDITRSVEDTSRRDQLLQQLTEGS
                     RAALANLRAAAETMQQYPDMDEPRRQRFFEVIADEARKLSGQLEEALSAQGDAPRTPW
                     LLEDMLADDLLFALRGNLPAGPRIVTSAQGDAAPLWLSVNSYAMVQALSHLASRLATT
                     FSLGELDLELGAVGRWVRLSLCWHGGALDPALLHEWESQPLAIGAVAGAASLKQVLDQ
                     HGAEMWFQADPADGRQRLCLQLPAAQPEQAAAVPQARGGRPVYYDFDLFSQPGQSPEL
                     DNRPLTELAYTVFDTETTGLAPSDGDEIISIGAVRILNGRLLEQEFFDRLVRPRAAVR
                     RESQAVHGIAPSMLVDQPFIEQVLPKFQRFAEDTVLVAHNAAFDMRLLQLAQARTGVV
                     FVQPVLDTLLLSALVHPGHADDHRLEQIAARLGVQVIGRHTALGDAIVTGEVFLKLIP
                     LLAERGIRTLKQAREASQRTRYAKLEY"
     gene            3925..5019
                     /locus_tag="VGA59_16100"
     CDS             3925..5019
                     /locus_tag="VGA59_16100"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEX9721242.1"
                     /translation="MTGAASAPTGAIEASTVGWLFAEPFGRIGGAIAAAAEIDELAGA
                     APRIREFARDMLGHGVGARQVSELLSQLNDRLTGRMLQIVAGEQHLDLGQACWLAFGS
                     QGRSEQTIVTDQDNGLVFESAEPARDRERWLALGRRMNEGLSACGYAWCKGQVMAGNP
                     ACCMTVAEWCDRFAEWIERGGPDELLKACIYFDLRPVAGRGQLAQPLTDLLAREPARV
                     PRFLKQMADNALANPVPLSWLGATETRKVAGRPMLDLKHHGTMVFVDAARLYALAYGI
                     ANTSTRERLQAIAPKLRVPPHESEAWVRGFEYLQLLRLQTQAGIGPDAPGNSNMLDVG
                     SLNDIDRRMLNETLRVARRLQQRMQLDYQR"
     gene            complement(5025..5831)
                     /locus_tag="VGA59_16105"
     CDS             complement(5025..5831)
                     /locus_tag="VGA59_16105"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX9721243.1"
                     /translation="MNFLRHYLIALRFSTRVPVSGTLAQWAGFSPDVLSASAAHFPGV
                     GWLVGIVACAAFAVLGLALPDSAFTALAAAVGSTIATVLLTGGSHENGLAHTADGLGG
                     GAAPQRALEIIADSRLGAFGAIAVALALLAKVSLLAVLAASSPVAVLVALLAGHVLSR
                     FWPLLLVRGMSCVGDAGSTGKGPLAERIGSRNLAIALTWCVLALLVALVAQGVTFAIF
                     SLTASAMALLYMKRLLARRLQGFTEESLGAAQQVCEVAFYFGAAVALAVD"
     gene            complement(5889..7433)
                     /gene="ilvA"
                     /locus_tag="VGA59_16110"
     CDS             complement(5889..7433)
                     /gene="ilvA"
                     /locus_tag="VGA59_16110"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEX9721244.1"
                     /translation="MKKHLNPADYLKKILTARVYDVAVESALEPAKNLSRRLHNKVLL
                     KREDQQAVFSFKLRGAYNKMAHLTAEQLRRGVICASAGNHAQGVALSAHRLGARSVIV
                     MPITTPQVKIDAVKALGAEVVLQGESYSDAYEHAVTLEKEQGLTFVHPFDDPDVIAGQ
                     GTIAMEILRQHQGPLDAVFVAIGGGGLIAGVANYIKAVRPEIKVIGVQMNDSDAMTRS
                     VAASKRITLPDVGLFSDGTAVKLVGEETFRVARELVDEFMTVDTDAVCAAIKDVFVDT
                     RSIVEPAGALAVAAIKQYVADKKTRGETYAAILCGANMNFDRLRFVAERAEVGEEREA
                     LFAVTIPEERGSFKRFCELIGTLPGGPRNVTEFNYRISDAAKAHVFVGLTTHGKGESA
                     KIAANFSRHGFEALDLTHDELAKEHIRHMVGGHSALARDERLLRFVFPERPGALMKFL
                     SHMRPGWNITLFHYRNQGADYGRILVGLQVPKSENKEFKEFLDTLGYPHVEETGNPVY
                     RLFLQT"
     gene            7718..8167
                     /locus_tag="VGA59_16115"
     CDS             7718..8167
                     /locus_tag="VGA59_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9721245.1"
                     /translation="MECTVSWTGATGTRSGMGFVAETGSGHVLAMDGAPDAAQPQNGG
                     QNLAPRPMEAVLAGTGGCSAYDVVLILKRGRHDVQGCSVKLTAERALEDPKVFTRIHM
                     HFTVTGRGVPAGAVERAIAMSHDKYCSASVMLGKTAEITTSFELVEV"
BASE COUNT         1378 a         2759 c         2792 g         1298 t
ORIGIN      
        1 tgggattcgc cttgtgggcc tacacgctgt tcgtgccggc gatcgtcaag tcgggctggc
       61 tgccgatgtc gacttcgttc atcaatgaag ggccgttcgg catcgcgctg ctcaagccat
      121 acgcgctgtt cggtctcgcg ggactcgacg agatcagcca ttcgctgctt tggacgatgc
      181 tcgccaatgt cggctgctac atcggcgtgt cgctgctcgg gcgcccgacg gtcagcgagc
      241 aggcgcaggc cgtgctattc gtcgatgtgt tcaagatggc cgagcgcggc atcgggttgt
      301 ggcgtggcag cgcctcgatg cacgacatca tcgtgctggt ggggcgcttc ctcggccccg
      361 cccgcgccga ggaggccttc gccggctatg cggcgcgccg tggattgaaa tccgcgcgcg
      421 agatcgagcc cgacgccgag ctcgtgcgct ttgccgagaa cctgctggcc ggcgccatcg
      481 gcgcgtcgtc ggcgcgcgtg atgctcgcgt cggccgtcaa ggaggaggcg ctcgggatgg
      541 acgaggtctt cgacatcctg gacgaggcct cccaggtgat cgcctacagc cgccagctgg
      601 agcagaagtc cctggaactc gaggcggcca ccgcggagct cacggccgcc aacgagagcc
      661 tgaaggaact cgatcggctg aaggacaact tcatcaccac cgtgacgcac gagctgcgca
      721 caccgctgac gtcgatccgg gctttttccg aaatcctgca cgacaacccg atgctggagc
      781 aggatcagcg catggaattc atctccatca tcgtcaagga aagcgagcgc ctcacgcgcc
      841 tgatcaacca ggtgctcgac ctggccaagc tcgagtcggg caatgccgaa tggcattcga
      901 ccgagctgga catgaacgag gtgatccatg aggccgtgtc gaccaccagc cagctgctgc
      961 gcgagagccg tgtggtgctg gcgctggacc tcccggcctc cgtgccgccc gtgcatgccg
     1021 accgcgaccg cctgatgcag gtgatgctga acctgatttc caatgcggtg aagttctgcg
     1081 accgcgccgc cgggcgcatc gagatcgctc tacgggtact gcccgatgcg ctgcaagtgg
     1141 acgtgcacga caatggcccg ggcgtcgccg cggcccacca ggaactgatc ttcgagaaat
     1201 tccggcaggt cggcgacacc ctcacggaaa aaccgcaggg caccggcctg ggcctgccga
     1261 tcagccggca gatcatcggc cacctcggcg ggaggctctg ggtcagcagc cgcccggggc
     1321 agggcgccac cttttcgttc acactgccgt tgaaacctcc gctgcgtcag tctccatccc
     1381 tggaccatcg accatgagca agaccgtgct gatcgccgac gacgaaccca acatcgtcat
     1441 ttcgctggag ttcctgctgg agcaggacgg ctaccgggtc ctgctggcgc gcgacggcca
     1501 tgaggcgctg gaggcgatcg agcgggaggt gcccgacctg gtcctgctgg acgtcatgct
     1561 gccgcgcctg agcggttacg acgtctgcca gcgcatccgg caaaaacccg agctcgcgaa
     1621 ggtgcgggtc atcatgctga ccgcgcgcgg ccgcgaagtg gaagtcacca aggggctggc
     1681 cctgggcgcg gatgcctatg tcaccaagcc gttctccaca cgcgatctgc tggtgcagat
     1741 ccgcaggcag ctcggggacg cgccgcccta gggaaccgtc accccgcccg atgcgatgag
     1801 tctgagatcg aaatacctcc tgtggctcgc gctttcgagc ctgctcgcgc tcgctctggt
     1861 ggcgatggtg ggcctggcgt tctggtccga cctccaggag cccgagcgcc gggcagtggc
     1921 ggacatgctg ggctcgcggc agctgctgct ggtcatgctg gcgctgctgc cgcccgtggc
     1981 catcggcgcg ttgctgcggc tggcgctggc ggcctatccg gcggccgcgg tcaagctggc
     2041 ggaagaggtg cgcctgattc actcggtcca cccggggcac cggctggctc ccacgggggc
     2101 gcccgaaatg caccagctgg gggccgcggt caacgagctg gccgaggctc atcgcgcgct
     2161 gcaccaggag gtgcaggcgc gcatcgagga ggccaacgcg catctcgcgc aggagaggaa
     2221 ccggctggcc gcactgatgt cggagctggc ccagagcgtg ctggtctgca acgtcgaggg
     2281 gcgcatcctg ctgtacaacg cgcgcgccag ccgggtgctg gagcaggcgg atggcgccga
     2341 ggtcccggtc ggcctgggcc gatcgatctt cggcatcctg gaccgcagcc cgatcatcca
     2401 cgcgctgggc cagctgggcc accggctcga gcagggcacg ccgcagccgg tcgcgcgatt
     2461 cgtcaccacg cgcggcgggc gcctgctgcg cgcgcagatg gcgccggtgc tcgaccagta
     2521 tggcgccatc accgggttcg tgctgatgct ggaagacatc acccgcagcg tcgaggacac
     2581 cagccggcgc gaccagctgc tgcagcaact gacggagggt tcgcgggcgg ccctggccaa
     2641 tctgcgtgcc gcggcggaaa cgatgcagca gtatccggac atggacgagc cgcggcgcca
     2701 gcgcttcttc gaggtcatcg ccgacgaggc ccggaagctg tccgggcagc tcgaagaggc
     2761 gctgtccgcg cagggcgatg ccccgcgcac gccctggctg ctggaagaca tgctggccga
     2821 cgatctcctg ttcgctttgc gcgggaatct gccggccggt ccgcgcatcg tcacaagcgc
     2881 ccaaggcgac gccgcgccgc tgtggctgag cgtcaacagc tacgccatgg tccaggcact
     2941 ctcccacctg gcaagccggc tcgccacgac attcagcctg ggcgagctgg acctggaact
     3001 gggggccgtg ggtcgctggg tccgcctgtc gctgtgctgg catggcggtg cgctcgatcc
     3061 cgccttgctg catgaatggg aaagccagcc cctggccatc ggcgccgtgg ccggggctgc
     3121 cagcctcaaa caggtgctgg atcagcatgg cgcggaaatg tggttccagg ccgatcccgc
     3181 cgacggcagg cagcggctct gcctgcagtt gcccgcggcc cagcccgagc aggccgccgc
     3241 ggttccgcag gcgcgtggcg ggcggcccgt gtattacgac ttcgacctgt tcagccagcc
     3301 ggggcagagc ccggaactcg acaaccgtcc gctgaccgag ctggcctaca ccgtgttcga
     3361 tacggaaacc accgggctcg cgccgtcgga cggggacgag atcatctcga tcggcgccgt
     3421 gcggattctc aacgggcgcc tgctggagca ggaattcttc gaccgcctgg tccggccgcg
     3481 cgccgcggtg cggcgcgagt cacaggccgt gcacggcatc gccccgtcca tgctggtcga
     3541 tcagcccttc atcgaacagg tgttgcccaa gttccagcgc ttcgctgaag acacggtgct
     3601 ggtggcccac aacgccgcgt tcgacatgcg cctgctgcaa ctggcgcagg cgcgcaccgg
     3661 cgtcgtgttc gtgcagccgg tgctcgatac gctcctgctg tcggcgctgg tgcatccggg
     3721 ccacgccgac gaccacaggc tcgaacagat cgccgcccgg ctgggtgtgc aggtcatcgg
     3781 ccggcacacg gcgctgggcg atgccatcgt gaccggcgag gtgttcctca agctcattcc
     3841 cctgctggcc gagcgcggta tccgaaccct caagcaggcc cgcgaggcgt cgcagcgaac
     3901 gcgctacgcc aaactggagt attgatgaca ggcgctgcat cggcaccgac cggcgcgatc
     3961 gaagcctcga ccgtggggtg gttgttcgcg gaaccgttcg gccggatcgg cggcgcgatc
     4021 gccgccgcgg ccgaaatcga cgagctcgcc ggcgcggcgc cgcgcatcag ggaatttgcc
     4081 cgggacatgc tgggtcatgg cgtgggcgcg cgccaggtga gcgagctgct aagccagctt
     4141 aacgacaggc tgaccggccg catgctgcag atcgtcgccg gggaacagca cctggacctg
     4201 gggcaggcgt gctggctggc gttcggctcg cagggacgca gcgagcagac catcgtgacc
     4261 gaccaggaca acggcctggt gttcgaaagc gcggaaccgg cccgggaccg cgaacgatgg
     4321 ctggcgctgg gacggcgcat gaacgagggc ctgagcgcct gcggctacgc gtggtgcaag
     4381 ggccaggtga tggccggcaa tccggcgtgc tgcatgacgg ttgccgagtg gtgcgaccgg
     4441 tttgccgagt ggatcgagcg cggcggcccg gacgaactgc tcaaggcctg catctatttc
     4501 gacctgcggc cggtggccgg ccgcgggcag ctggcgcagc cgctcacgga tctcctggcg
     4561 cgggagccgg cccgggtgcc gcgcttcctc aagcagatgg ccgacaatgc gctggccaac
     4621 ccggtgccgc tgagctggct cggtgccacc gaaacgcgca aagtggcggg acggccgatg
     4681 ctggacctca agcaccacgg aacgatggta ttcgtcgacg ccgcgcgcct gtacgcgctc
     4741 gcatacggca tcgcaaatac cagcacgcgc gagcgcctgc aggccatcgc gccgaagctg
     4801 agggtgccgc cacatgagag cgaggcctgg gtccgcggct tcgagtacct gcaactgctg
     4861 cggctgcaaa cgcaggcggg catcggcccg gacgctcctg gcaactccaa catgctcgac
     4921 gtcggctcgc tcaacgatat cgaccgccgc atgctcaacg agaccctgcg cgtggcgcgc
     4981 cggctgcagc agcgcatgca gctcgattac cagcgctgaa gacctcaatc caccgcgagc
     5041 gccacggccg ccccgaagta gaaggcgacc tcgcacacct gctgggctgc gcccaggctt
     5101 tcctcggtaa atccctgcag ccggcgcgcg agcagccgct tcatgtacag cagtgccatg
     5161 gcgctggcgg tgaggctgaa gatcgcgaac gtgacgccct gggccaccag ggcgacgagc
     5221 agcgccagca cgcaccaggt gagcgcgatg gccaggttgc gcgatccaat tcgttcggcc
     5281 agcggaccct tcccggtcga gcccgcatcg ccgacgcagg acatgccgcg caccagcagc
     5341 agcggccaga agcgcgacag cacatgtccg gcgagcagcg ccaccagcac ggcaaccggt
     5401 gacgaggcgg ccagcacagc cagcagcgac accttggcga gcagggccag ggcgacggcg
     5461 atggcaccga aggcgcccag gcgcgagtcc gcgatgatct ccagcgcgcg ctgcggtgcg
     5521 gcgccgccgc cgaggccatc cgccgtgtgg gccaggccgt tctcgtgcga gccgcccgtc
     5581 agcagcaccg tcgcgatggt cgagcccacc gccgcggcca gggccgtgaa agcgctgtcg
     5641 ggcagggcca ggcccagcac cgcgaaggcg gcgcaggcga cgatgcccac cagccatccc
     5701 acgcccggga agtgcgccgc gctggcactg agcacgtcgg ggctgaagcc ggcccattgc
     5761 gccagcgtac ccgacaccgg cacgcgggtg gagaatcgca gggcgatcag gtaatggcgc
     5821 aggaagttca ctcacgcatg gtagcggccc aggacgcgcc tgggtccgcg gcgcgccccg
     5881 cccctctgct atgtctgcag aaacaagcgg tagacggggt tgccggtctc ctcgacatgc
     5941 gggtagccca aggtgtccag gaactccttg aattccttgt tttcgctttt cggcacctgc
     6001 aggccgacca ggatgcggcc gtaatcggcg ccctggttgc ggtagtggaa gagcgtgatg
     6061 ttccagcccg gccgcatgtg cgagaggaac ttcatcagcg cgccggggcg ctccggaaag
     6121 acgaagcgca gcaggcgctc gtcgcgcgcc agggccgaat ggccgcccac catgtggcgg
     6181 atatgttcct tggccagctc gtcgtgcgtg aggtccagcg cctcgaagcc atggcggctg
     6241 aagttggcgg cgattttggc cgactcgcct ttgccgtgcg tagtgaggcc gacgaacaca
     6301 tgggccttgg cggcgtcgct gatgcggtaa ttgaattcgg tgacgttgcg cgggcccccg
     6361 ggcagcgtgc cgatcaactc gcagaagcgc ttgaagctgc cccgctcctc gggaatggtg
     6421 acggcgaaca gcgcctcacg ctcctcgccc acttcggcac gctcggccac gaagcgcagc
     6481 cggtcgaagt tcatgttggc cccgcacagg atggcagcat aggtttcccc gcgcgtcttc
     6541 ttgtccgcca catattgctt gatcgccgcc accgccagcg cgccggccgg ttccacgatg
     6601 ctgcgcgtgt cgacgaagac gtccttgatc gcggcgcaca cggcgtcggt gtcgacggtc
     6661 atgaactcgt cgaccagctc gcgggccaca cggaatgtct cctcgcccac cagcttcacc
     6721 gccgtgccgt cggagaacag gccgacgtcg ggcagcgtga tgcgcttgct ggccgcgacc
     6781 gaccgggtca tcgcgtcgga atcgttcatc tgcaccccga tcaccttgat ctcgggccgc
     6841 accgccttga tgtagttggc cacgccggcg atcagcccac cgccgccgat ggccacgaac
     6901 acggcgtcca gcggcccctg gtgctggcgc aggatctcca tggcgatcgt tccctgcccg
     6961 gcgatcacgt cggggtcgtc gaacggatgc acgaaggtca ggccctgctc cttttcgagc
     7021 gtgacggcat gctcatacgc atcggagtag ctctcgcctt gcagcacgac ctcagcgccg
     7081 agcgccttga ccgcgtcgat ctttacctgg ggggtggtga tcggcatcac gatcacggac
     7141 cgcgcgccca gccgatgcgc cgacagcgcc acgccctgcg cgtggttgcc ggcggacgcg
     7201 cagatcacgc cgcgccgcaa ctgctcggct gtcaggtggg ccatcttgtt gtaggcgccg
     7261 cgcaacttga aactgaagac cgcctgctgg tcctcgcgtt tgagcaggac cttgttgtgc
     7321 aggcgccggc tcagattctt cgcaggctcc agggccgact ccacagccac gtcgtacacc
     7381 cgcgcggtaa gtattttttt caggtaatcg gcggggttga ggtgcttttt catgaccaaa
     7441 ggatgataag cgccatgaaa aaagcccctg ggccttgcga tccaggggct aaatccacca
     7501 aaggagaggt ggaggagaca acctgcaggt tgcggcattg gttgacaatt gccttgccgg
     7561 cccaagccgc gaccagtgca caacgaatcg ttatgctgcg aacccagtat agccgcagac
     7621 ttgctgcatc gcagcaagcc catctgtctc aaactgagac agggcgccaa ctattagcgg
     7681 aaataattcg atccaagcca cttcgatagg gaacaccatg gaatgcacag tcagctggac
     7741 aggcgcaacg gggacccgtt caggcatggg tttcgtggcc gaaaccggca gcggccatgt
     7801 gttggcgatg gacggtgcgc cggacgccgc ccagccgcaa aacggcggcc agaatctggc
     7861 accgcggccc atggaggccg tcctggccgg caccggcggc tgctcggcct acgacgtggt
     7921 gctgatcctc aagcgcggcc gccacgacgt tcagggctgc tcggtgaagc tcacggccga
     7981 gcgtgccctc gaggatccca aggtgtttac caggattcac atgcacttca cggtgaccgg
     8041 gcgcggtgta ccggccggtg ccgtcgaacg cgcgattgcg atgagccacg acaagtactg
     8101 ctcggccagc gtcatgctgg gcaagacggc cgagatcacg acgagcttcg aactcgtcga
     8161 agtctagtgc cgctgtccgt gaacttgtca tcccgggctt gaccaagttg tcatcccggg
     8221 cttgacc
//
LOCUS       DASWIY010000452        21505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2915178, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000452 DASWIY010000000
VERSION     DASWIY010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 21505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21505
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2915178"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..578)
                     /locus_tag="VGA59_16120"
     CDS             complement(90..578)
                     /locus_tag="VGA59_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX9721246.1"
                     /translation="MPALDWVFLAVLAASLLLGAWRGLVYEVLSVMSWIAAFVVAQWL
                     APDAAALLPVERAPEAGRYAIGFVVVFIAVVFVGAVLAWLTKKLVQAVGLRPVDRTLG
                     AMFGLIRGVVLLLAVAVVVNMSPARRAVWWTQAKGAAVSMAVLEELKPLLPERFGRYL
                     PG"
     gene            complement(593..1309)
                     /locus_tag="VGA59_16125"
     CDS             complement(593..1309)
                     /locus_tag="VGA59_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX9721247.1"
                     /translation="MAFFKMRKGGDESPATRRPPESIEAMRRRARHRLIGAGVLVLVG
                     VIGFPLLFDTQPRPIAVDIPIEIPDRNKVKTLPAPAQPAAQAPTVSGPVTVAPRPPAA
                     PEATAAVTPQPVPPAAVAEAPPTTKPEPKVAAQPELKKDEGAKAKALLEGRPVQVAAA
                     TDEGRYVVQVGAFADPAKARAARLKVERAGLKTYTNVADTKDGKRVRVRVGPFVTRAE
                     ADKAAQKLKGLDLPAAILTL"
     gene            complement(1332..2627)
                     /gene="folC"
                     /locus_tag="VGA59_16130"
     CDS             complement(1332..2627)
                     /gene="folC"
                     /locus_tag="VGA59_16130"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEX9721248.1"
                     /translation="METLQDWLAHCETLHPKTIQMGLERVQAVAARMGGLRFDCPVIT
                     VAGTNGKGSTCAMLEAIVLQAGYRTGLYTSPHLVHFEERCRVHGESVQAQALVPHFER
                     VEAARHDVSLTYFEFTTLAIMSLMAGATLDLAILEVGLGGRLDAVNIVDADCAVITSI
                     ALDHMDYLGCDRESIGREKAGILRTGKPAIVSDPTPPQSVLDRAIDIGADLWTLGRDF
                     NFSGDKQQWAWAGRGRRYAGLAYPALRGANQLVNASGALAALEALRARLPVAAQAVRN
                     GLAMVELPGRFQIVPGQPTLVLDVAHNPHSVAALAANLDAMGFYPATHGVFGAMADKD
                     LAAMLKKMGPLIDRWYFTDLPAARAGTAAALLAMWQSQETTRRGVPASAHADPMEALR
                     CAVAAADPADRIVVFGSFYTVGGVLKDGLPRLQARHIAR"
     gene            complement(2674..3882)
                     /locus_tag="VGA59_16135"
     CDS             complement(2674..3882)
                     /locus_tag="VGA59_16135"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9721249.1"
                     /translation="MTRPIQTARQIARLAAKEWLLLMRNPHGLAVLFLMPAVFVLIMS
                     FTLKNTLVARVDLPVTGWVMEDTTAIAGQWAKEWFDRHGGQRFASRDELQAALKTRRV
                     EAGVVVRSPLMGADGRPHIEQLELWLGNLVQPAAAARLRAELSFSLLQVQMKIAAAEA
                     GPFASVLLASAGSTELSAQGTPKVRYLYEIESGRAMTAVQQSVPAWLIFGMFFVVIPI
                     AGVLIQERNEGTLARLATFKVSMGAVLGGKLLAFILLNWVQLAFMLVMGRWIVPLLGG
                     DALSLDIHPGWFLLVVVATSAAAVGLALLVAAYTRNFDHAAALGGGLNVVLAAIAGVM
                     VPRMLMPQGLQRLSEWSPMGWALDAMQSVFLGSPSAGHLLPRLGLLSGFAALCMVLSW
                     WALRNTKGAR"
     gene            complement(3890..4630)
                     /locus_tag="VGA59_16140"
     CDS             complement(3890..4630)
                     /locus_tag="VGA59_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9721250.1"
                     /translation="MTDGPAVIAAEQLVYRYDKQAEPALCGVDLSIERGQAVGLLGPN
                     GSGKSTLINLLAGLRTPQGGRIRYPGPGVPSVAWVPQEYAFYPELTCLENLRFFAAML
                     ALTGAQAQRRVDGAIQTCMLQEFAGRRARHCSGGVRRRLNLGIALLQQPEVLLLDEPT
                     VGVDPQSRAFLLDRVRDMVRDGTAVLYATHYMEEVSAVCSHIVLLDHGRVLASGDLAT
                     LLRGPAGSAPFDHLEALFMHYTRRRLRD"
     gene            complement(4627..5475)
                     /locus_tag="VGA59_16145"
     CDS             complement(4627..5475)
                     /locus_tag="VGA59_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9721251.1"
                     /translation="MEAVRQESAGPSWDGVWIAIPAYQEEGTIRALCQEALVQCPRVI
                     VVDDGSSDGTRAQLQHLPVKLLVHPENRGKAASLCTAFAYALAQRASCVIALDGDGQH
                     DPADAAGLLAAWRQHPERVVIGSRLHDRSQFPPARYRANRFACFWISWAAGHSIADSQ
                     SGFRVYGREVMQIALGGKVRGDRFTFESEILIEAARRGHPTLAVAIPGRYPVHARASH
                     FRPVIDIAKIVVMVAGRLLRQGMAPLGLWRSLKPAPVLPGRTAAAASTPVPPQRIPRT
                     TDAPVR"
     gene            complement(5478..6218)
                     /gene="fabG"
                     /locus_tag="VGA59_16150"
     CDS             complement(5478..6218)
                     /gene="fabG"
                     /locus_tag="VGA59_16150"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX9721252.1"
                     /translation="MTGSAAPAVRRALVTGGSGGIGAAICRALASQGVHVIVHANRGI
                     ERAQALVAALVAAGGRAEPIAFDLADDEQVRAAVESVLSAGPIQILVNNAGVHRDALL
                     AGMSPAAWHEVIDTNLNGFFRVTQPLLLPMVRTRWGRIVNITSVAGQAGNPGQGNYAA
                     AKAGLHGVTKSLALELAGRGITVNAVAPGIIDTDMAAAFAPEKIKAMVPAGRAGRPEE
                     VAAVVAFLASDAASYLTGQIIGVNGGLY"
     gene            complement(6215..6649)
                     /locus_tag="VGA59_16155"
     CDS             complement(6215..6649)
                     /locus_tag="VGA59_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HEX9721253.1"
                     /translation="MMPDREAIERMVPHAGSMCLLEAVTAWDAGHIACSAAEPGAGHP
                     LARDGCVPAVAACEYAAQASAVHGALLDDAHGPRAGMLAKLMEVRLHSRCFPAGGGPL
                     AVRAELLGRTAAGCLYAFDVASIGTPVASGRLMVAFAPVGRP"
     gene            complement(6646..7461)
                     /locus_tag="VGA59_16160"
     CDS             complement(6646..7461)
                     /locus_tag="VGA59_16160"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEX9721254.1"
                     /translation="MTSLSFRLHAAGVLAPGLPSLPELRAVSSGAAPYTPAPLKLPSP
                     ALLPAHERRRASQPVRLVLACIEQALETCPFPVRDMRSVFATDEGTGEVCQQMLDTLA
                     TTRQVSPLLFPNSVLNAPSGYFSIAWRNQQPATVVSLGLESFASGLLCAVTEAQSTAQ
                     PVLLVVYDPAMTSPLSELLPVQEATASAWIISSGPVDESVPVLCAFSIAMEPAGTQSA
                     TPLPAWLPASWVAHSSARGLAALGLLEAEAGTPCRLSLGGQILTLQRTQEGRA"
     gene            complement(7463..8713)
                     /locus_tag="VGA59_16165"
     CDS             complement(7463..8713)
                     /locus_tag="VGA59_16165"
                     /inference="COORDINATES: protein motif:HMM:NF006618.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX9721255.1"
                     /translation="MASAATTPCWSAARRPDPRSFSYRTPVLGISATTLVHPAGHGTR
                     ALWDSISRGRTGLQPNALPWCELACWVGAVPGVEDPALSAHLGAWDCRSHRLAWLALQ
                     DAPFRSALAAAIGRHGAHRVGLVLGTSTSGIRSTESAYAHRRETGRWPEAFDYRKTHA
                     VDALCRFSAQLLGLQGPMAAISTACSSSAKVFLTAQRWIDAGLIDAAVVGGADSLCLT
                     TLHGFDALQLLSSDMCRPFDLARNGISIGEAAGFVLLERRPAAVQFAGGGESSDAWHM
                     STPHPEGEGAQAAMRMALSAAGIGPSDIGYVNAHGTATPANDRAEAVALSAVFGPHAV
                     PVSSTKGATGHTLGAAGIVEAIVTAQALEHQALPPSTNWMTADPALAVNLVRQPASAR
                     MQHAMSNNFGFGGSNCTLVFTLAQ"
     gene            complement(8664..9731)
                     /locus_tag="VGA59_16170"
     CDS             complement(8664..9731)
                     /locus_tag="VGA59_16170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9721256.1"
                     /translation="MYDKDPLTALEAVTAAQRLAFAPLAFQATAVMRDRGVLAAIATA
                     GPVQGRTTDEVAASTGLSVYAVRVLLEAGLGLHIVWRQGDRFFLGKLGRFLLDDEMTR
                     VNFDFTRDVCYRAAAHLDQSLMQGRPVGLKEIGPWDTLYEGLSVMPEPSRRSWHAFDH
                     FYSDGAFPAACERLASLPPQRLLDVGCNTGRWAQVCLTRLPALEVGLVDLPLQLERAR
                     ERLAHAGLGERATLHPVDLLDPAAALPAGYDLIWMCQFLDCFADEQIVGILRKARAVL
                     APGGRVWILELFWDRQRFEAAAFSLQQTSLYFTCVANGNSQMYDSSVFVALVERAGLE
                     VSAVTDGVGGYHTLLECRPAA"
     gene            complement(9758..10336)
                     /locus_tag="VGA59_16175"
     CDS             complement(9758..10336)
                     /locus_tag="VGA59_16175"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HEX9721257.1"
                     /translation="MIKAFLPRGGFMLVLACLGIAMAETAALTVAQLQRALQAAARPS
                     VSYQEVRESPWLSTPLTSRGTMRSTPTALEKRVESPREETWRLLADRAEWVGPGATGS
                     KQILFTHAPALAALSQVMRRVVAGDLVALQRDFEIKLTGDERVWSAQLQPRSADVARH
                     LDHVELQGTAGQLHVIIVVERQGERTITRLQP"
     gene            complement(10333..11223)
                     /locus_tag="VGA59_16180"
     CDS             complement(10333..11223)
                     /locus_tag="VGA59_16180"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721258.1"
                     /translation="MKGWSDKAERGSAWLIELIAWLARTVGRPACRALLYPITFYFFL
                     TDAGARRASTEFLSAVHGRPVRQNEVFGHIYSFAATLLDRVYMAAGEFDRFQVTIEGL
                     ALVDQAMEAGRGCVLLGSHLGSFDLMMLAHRAMDGRPVSVMMRMDPRARLRRIAGIDE
                     QALNLIPLGRPDSYLRAHEALTRGEIIGILADRVDGAATALAAPFLGRRVTMPIAPHV
                     LAARSEACVLMCFGLYEGGNRYRIEFVEFGPAAPRQARGAALQPAVDRYATVLEQYAR
                     RYPLNWFNFYPYWAAGEARA"
     gene            complement(11220..11537)
                     /locus_tag="VGA59_16185"
     CDS             complement(11220..11537)
                     /locus_tag="VGA59_16185"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721259.1"
                     /translation="MSRLEFDLRVDSDHPSLEGHFPGNPVVPGVLLIDRVQQALCRLT
                     GRELVQLKQVKFASALRPGEAAQVLLDMDGERVSFRISARRGDAAPLVAEGVGILSAE
                     APA"
     gene            complement(11534..12919)
                     /locus_tag="VGA59_16190"
     CDS             complement(11534..12919)
                     /locus_tag="VGA59_16190"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9721260.1"
                     /translation="MQYLADTPLTAAPRYLPQPALIAAQHGWTWREVHAASLALARRL
                     EGANTVCNLCGSRVGFLVAWLASLRRGCLQLLPPSGGHTDLAAILQSSASPVIVVDDE
                     NLLQPHWSDHARCIVHRPQVPSASAPDAGLAWSPDWDAPSIRLYTSGSTGAPEPQTKT
                     LGEFCRGALVLAARLDEVVEGGTAGLRQIVCSVPPQHMFGVETSVMLPLVTGIPVIDR
                     RPLLAADVHAAFRDAPGEGAWIATPLHLSALARSGQEVPRCRLVLVSTMPLAPGLAEQ
                     AETLARAPVMEIYGSTETGVVAMRRTARQAHWRPVDGVRIEPAPDGAQVWGTHFASPR
                     ILADQVELDGEGGFRLLGRQADLIKIAGRRASLAGLNLLLQDLPGLVDGVFYLPATGS
                     PTERLVLIHAGAPLDRAATRRWLRERMDPAFLPRTIIEVERLPRTDAGKLTRAALDAI
                     YAAKNPKAVGR"
     gene            complement(12927..13331)
                     /locus_tag="VGA59_16195"
     CDS             complement(12927..13331)
                     /locus_tag="VGA59_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721261.1"
                     /translation="MDWLQSLDALTLWRAVSLLVLGWLAYFFGRTLRPGAIPLIERIA
                     TVSDPEMTQPLRRYTRRLTAVWCAYFVAAALLSIAAAQYPLRAGAWVWAGTAALFVGE
                     HWLRPFFFPGRVFPSLTQQLRDTWSVWHPGRG"
     gene            complement(13339..13599)
                     /locus_tag="VGA59_16200"
     CDS             complement(13339..13599)
                     /locus_tag="VGA59_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX9721262.1"
                     /translation="MSARDLATLVVEGLNLEDVNPDEVDLLAPLFGGGLDLDSLDVLE
                     ISLIVQQRYGVKLKADDPDNEAIFASLQSLAAHIAGNLAAQR"
     gene            complement(13629..14018)
                     /locus_tag="VGA59_16205"
     CDS             complement(13629..14018)
                     /locus_tag="VGA59_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721263.1"
                     /translation="MRFHITLAALAVAIAVAGCTAVPIQNVSQTPATNATGKALSKDQ
                     VRGAIVRAGAALGWQMRDEGPSMLVGTLQLRKHTAVVEIPYSASSYSIKYRSSENLDE
                     KGGNIHKNYNGWIQNLTRGITAQLSAS"
     gene            14288..14638
                     /locus_tag="VGA59_16210"
     CDS             14288..14638
                     /locus_tag="VGA59_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEX9721264.1"
                     /translation="MVTLYGIPNCDTVKKARAWLAEHDVACEFHDFKKQGVPVAHLDA
                     WIAQLGWEKLVNRKGTMWRRLEPATQAGVTDAAGAKALMLAHPSVIKRPVVEWGGKTT
                     VGFDDAQWALLAGR"
     gene            14744..15472
                     /locus_tag="VGA59_16215"
     CDS             14744..15472
                     /locus_tag="VGA59_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX9721265.1"
                     /translation="MKKTILLSAMGVLSAFGSVAGAQEIGRVLSSTPVIQQVAVPRQV
                     CNYQPMAVQQPNSGAGGVVGALAGGAIGNQIGHGDGRAVATILGVVGGALLGNQIESN
                     NNAPQVQNVQSCTTQTYYENRTVGYNVLYEYAGKQYDVQMPNDPGPTIRLQLTPMGDS
                     GAAPEMAGSLSPAGSPGVIVAPPMTQAAGQPVAQVVAVPPAAYPAYYPAYSPAYYPRP
                     YYYPPIGLSLNFGFSGGHRHHRWR"
     gene            15814..16131
                     /locus_tag="VGA59_16220"
     CDS             15814..16131
                     /locus_tag="VGA59_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HEX9721266.1"
                     /translation="MTTQKIDGACITAQANVYFDGKCVSHSLTLADGSKKSVGVVLPS
                     ELTFNTGAPEIMECVAGGCEYKLAASSDWLKSGPGERFSVPGNSSFQIRVSQAYHYIC
                     HFG"
     gene            16233..17570
                     /gene="argG"
                     /locus_tag="VGA59_16225"
     CDS             16233..17570
                     /gene="argG"
                     /locus_tag="VGA59_16225"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX9721267.1"
                     /translation="MSTILEHLPTGQKVGIAFSGGLDTSAALHWMRQKGAIPYAYTAN
                     LGQPDEPDYDDIPRKALQYGAEKARLIDCRLQLAHEGIAALQAGAFHISTAGATYFNT
                     TPLGRAVTGTMLVAAMKEDDVHIWGDGSTFKGNDIERFYRYGLLTNPSLKIYKPWLDQ
                     AFIDELGGRAEMSAFMQQSGFAYKMSAEKAYSTDSNMLGATHEAKDLEQLSTGIKIVN
                     PIMGVAFWKDDVAVKAEEVRVRFEEGQPVALNGMTFADPVELILEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGLALLYIAYERLVTGIHNEDTIEQYRDNGRRLGRLLYQG
                     RWFDPQAIMLRETAQRWVARAITGEVTLELRRGNDYSLLDTQSPNLTYKPERLTMEKG
                     ESSFSPRDRIGQLTMRNLDIVDTREKLGVYTKAGLLSVGGGAALPSLGDGHSD"
     gene            17722..18069
                     /locus_tag="VGA59_16230"
     CDS             17722..18069
                     /locus_tag="VGA59_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HEX9721268.1"
                     /translation="MKHWQDRTNAVLGGWLIVAPMALGFQSDVGALATTMAVGLLLLA
                     TALGAIFVPRAWEEWTETALGLFLAVSPWVVGFSTPSARVTAVVTGLVALTLALWALQ
                     DSGQSTAGESTAQ"
     gene            18240..18605
                     /locus_tag="VGA59_16235"
     CDS             18240..18605
                     /locus_tag="VGA59_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HEX9721269.1"
                     /translation="MKQMKHWQDPVMAVLGVWLILSAWVLGFQDNLNALANAVIIGIL
                     LIAAALGAIFVPRAWEEWTEVVLGLWLAVSPWVLGFSAHPNATVATVVTGLAIIVLSL
                     WVLATDKDFAGWRPDRPAQ"
     gene            complement(18692..19732)
                     /gene="murB"
                     /locus_tag="VGA59_16240"
     CDS             complement(18692..19732)
                     /gene="murB"
                     /locus_tag="VGA59_16240"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX9721270.1"
                     /translation="MFVEQNVPLQPHNSFGIVAKARALVRVRGEADVREVLVSPDHGS
                     IPKFVLGGGSNIVLTGDVKPLVLKVEVMGRRLVEDGPRATIVEGGAGENWHDFVTWTL
                     DTGYPGLENLALIPGTVGASPVQNVGAYGVELQDRFESLDAMDLFTGKTYTLDAGQCA
                     FGYRDSVFKHVAAKEGDFGLGGRALILRVRFRLPKPWKPVLGYVDLQRKIQETGIHAP
                     PARQIYDWVCAVRKAKLPDPSVIGNAGSFFKNPTVTPEQCQDIIAREPNIVHYPMPDG
                     SVKLAAGWLIDACGWKGKSVGNAGVYEKQALVLVNRGGATGGEVVTLARAIQTSVYER
                     FGIRLEPEPVVI"
     gene            19776..20261
                     /locus_tag="VGA59_16245"
     CDS             19776..20261
                     /locus_tag="VGA59_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX9721271.1"
                     /translation="MPSFDTVCEANMVEVKNAVENTAKEIGTRFDFKGTSAAIELKDK
                     DITVFGDAEFQLTQVEDILTGKLAKRNVDVRFLDKGDVQKIGGDKVKRVVKVRNGIES
                     DQARKIARLVKDSKLKVQAAIQGEAVRITGAKRDDLQAAMALIRKEVPDLPLSFDNFR
                     D"
     gene            20433..21065
                     /locus_tag="VGA59_16250"
     CDS             20433..21065
                     /locus_tag="VGA59_16250"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX9721272.1"
                     /translation="MICFVALFASAPAGAQSVALQGMLGAKALLIVDGTAPRSVAPGD
                     THQGVKVISTSGDQAVVEIAGKRHTLRVGDAPASVGARTGGDARGSRIVLTAGSGGHF
                     TTQGAINGQAVQFLVDTGATSISMGVPEAQRIGLNYKAGQLGWGSTANGNVAVWHVKL
                     GSVRIGDVVINDVNAAVLPSAMPYVLLGNSFLSRFQMKRDNDQMVLERRY"
     gene            21083..>21505
                     /locus_tag="VGA59_16255"
     CDS             21083..>21505
                     /locus_tag="VGA59_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9721273.1"
                     /translation="MPDHLTDSVAPPSEDSRSALAPLRRPVFRMLWLTWMAANTSMWM
                     NDVAAAWLMTSLAPSPLWVALVQSAATLPVFLLGLPSGALADILDRRRYFMMTQLWVA
                     AVASILCVTVMMGWMTAPLLLALTFANGVGLAMRWPVFA"
BASE COUNT         3645 a         7388 c         7194 g         3278 t
ORIGIN      
        1 ttccacacat gttgatgctt tcatcaatct gaagactgcg ctgcacgttg tttcgcgcag
       61 tctacaaggc aaggccggca gcccaaccgc taacccggca gataccgccc gaaacgctcg
      121 ggcagcaacg gcttcaattc ctcgagtacc gccatcgaaa ctgccgcgcc cttcgcctga
      181 gtccaccaca ccgcgcgccg tgccggcgac atgttgacta ccaccgccac ggccagcagc
      241 agcaccacgc cccggatcag gccgaacatg gcgcccagcg tgcggtccac cggccgcaag
      301 ccgaccgctt gcaccagctt cttggtcaac caagccagca ccgcgccgac gaataccacc
      361 gcgatgaaca ccaccacgaa cccgatcgcg tagcgcccgg cttccggcgc gcgctccacc
      421 ggcaacaggg cggcggcatc gggtgccagc cactgcgcga cgacgaacgc cgctatccag
      481 ctcatcaccg acaacacttc gtacaccagc ccgcgccacg cgcccaacag cagcgaagcc
      541 gcgagcacgg ccagaaatac ccagtccagc gcaggcatgc gaggggctgg ccttacaagg
      601 tgaggatggc ggccggcaaa tccagcccct tgagcttctg cgccgccttg tcggcttcgg
      661 cccgggtgac gaacggtccc acccgcacgc gtacgcgctt gccgtccttg gtgtcggcca
      721 cgttcgtata ggtcttcaac ccggcgcgtt cgaccttcag ccgggccgcg cgggccttgg
      781 ccgggtccgc gaaggcgccc acctggacca cgtagcggcc ttcgtcggtg gcggcggcga
      841 cctgcaccgg cctgccttcg agcaaggcct tggctttcgc gccctcgtct ttcttgagct
      901 cgggctgggc cgccaccttg ggctcgggtt tggtggtcgg cggcgcttcc gcgacggccg
      961 cgggcggcac cggctgcggg gtcacggcgg cggtggcttc cggtgccgcc ggcgggcggg
     1021 gcgccaccgt caccggtccg gacacggttg gcgcctgggc ggcgggctgt gccggagcgg
     1081 gcagcgtctt gaccttgttg cggtccggga tctcgatcgg gatgtccacc gcgatcggtc
     1141 gcggctgggt gtcgaacagc agcgggaagc cgatcacgcc gaccaggacc agcacgcccg
     1201 cgccaatcag ccggtgcctg gcgcgcctgc gcatcgcctc gatgctttcc ggcgggcggc
     1261 gggtggccgg cgactcatcg ccacctttgc gcattttaaa aaaggccatg aaacctgtcg
     1321 ggcggttgga atcagcgagc gatatgtctg gcctgaaggc ggggcaagcc gtccttcaga
     1381 acgcccccca ccgtatagaa cgatccgaag acgacgattc tatcagcggg gtccgcagcg
     1441 gcgactgcgc agcgcaaggc ttccatggga tcggcatggg cgctcgcggg cacgccgcgg
     1501 cgcgttgttt cctgcgactg ccacattgcc agcaaggcgg ccgccgtgcc ggcgcgggcc
     1561 gcgggcaggt cggtgaaata ccagcggtcg atcagcgggc ccattttctt gagcatggcc
     1621 gcaaggtcct tgtcggccat cgcgccgaac acgccgtgcg tggccggata gaagcccatg
     1681 gcatcgaggt tggccgccag cgccgcgacc gaatgcgggt tgtgcgccac gtccagcacc
     1741 agcgtcggct ggccgggcac gatctggaaa cggcccggca gttccaccat ggccaggccg
     1801 ttgcgcaccg cctgcgccgc gaccggcagc ctcgcgcgca acgcttccaa tgcggccagc
     1861 gcccccgacg cattgacgag ctggttggcg ccgcgcagcg ccggatacgc caggccggcg
     1921 tagcgccggc cccgcccggc ccaggcccat tgctgcttgt cgccggaaaa attgaagtcg
     1981 cgccccagtg tccagagatc cgcgccgatg tcgatagcgc gatccagcac gctttgcggc
     2041 ggcgtgggat cgctcacgat cgccggcttg ccggtgcgca ggatgcctgc cttctcgcgg
     2101 ccgatgctct cgcggtcgca gcccaggtaa tccatgtggt ccagcgcgat gctggtgatg
     2161 accgcgcaat ccgcgtccac gatgttcacc gcgtccaggc gcccgcccag gccgacctcg
     2221 agaatcgcca ggtccagggt ggcgccggcc atgaggctca tgatggccag cgtggtgaac
     2281 tcgaaatagg tcagcgagac gtcgtgccgc gcggcctcga cccgctcgaa atgcggcacc
     2341 agcgcctgcg cctggacgct ctcgccatgg acgcggcagc gctcctcgaa atgcacgaga
     2401 tggggcgagg tgtacaagcc ggtccggtag ccggcttgca gcacgatcgc ttccagcatc
     2461 gcgcaggtag accccttgcc gttggtgccc gccaccgtga tcaccgggca gtcgaaacgc
     2521 aggccgccca tccgcgccgc caccgcctgg acgcgctcaa ggcccatctg tatggttttg
     2581 ggatgcaggg tttcgcagtg cgccagccag tcttgcagtg tttccatgga accgattgtc
     2641 ccagcactgc gatccctgcc cggctgcaaa ggtctacctg gcacccttcg tgttccgcag
     2701 ggcccaccaa ctgagcacca tgcacagtgc ggcgaagccc gacaacagcc ccaggcgcgg
     2761 cagcaaatgc ccggcgctcg ggctgccgag gaacaccgac tgcatcgcgt ccagcgccca
     2821 gcccatcggc gaccattcgg agaggcgctg cagcccctgc ggcatcaaca tgcgcggcac
     2881 catcacgccc gcgatggcag ccagtaccac attgaggccg ccgcccaggg cggccgcatg
     2941 gtcgaagttg cgggtgtagg cggcgaccag cagggccagc cccacggccg cggcactcgt
     3001 ggccaccacg accagcaaga accagcccgg gtggatgtcc aggctcaagg cgtccccgcc
     3061 gagcagcggc acgatccagc gccccatgac gagcatgaag gccagctgta cccagttcag
     3121 gaggatgaac gcaagcaact tgccgcccag caccgcgccc atggagacct tgaaggtggc
     3181 cagccgcgcc agcgtccctt cgttgcgttc ctggatcagc actccggcga tcgggatgac
     3241 gacgaagaac atcccgaaga tgagccacgc cggcacgctt tgctgcacgg ccgtcatggc
     3301 acggccggat tcgatctcgt acaggtagcg taccttgggc gtgccctgcg cggacagctc
     3361 ggtgctgccg gcgcttgcca gcagcacgct tgcgaacggg ccggcctcgg ccgccgcgat
     3421 cttcatctgc acctggagca gcgaaaagct gagttcggcg cgcaggcgcg cggccgccgc
     3481 cggctgcacc aggttgccca gccacaattc gagctgctcg atgtgcgggc gcccatccgc
     3541 gcccatcagt ggcgagcgaa cgaccacgcc cgcctccacc cggcgcgtct tcagcgctgc
     3601 ctgcagttca tcgcgcgagg caaagcgctg cccgccatgc cgatcgaacc attccttggc
     3661 ccattggccg gcgatcgcgg tcgtgtcctc catcacccaa cccgtcaccg gaagatccac
     3721 gcgcgcgacc agcgtgttct tcagcgtgaa cgacatgatg agcacgaaca cggcgggcat
     3781 gaggaacagc accgccagcc catgcggatt gcgcatcagc agtagccact ccttggcggc
     3841 aagccgggcg atttgcctcg cggtctggat ggggcgcgtc atgacgggtt caatcgcgca
     3901 gccgccggcg ggtgtagtgc atgaacagcg cctccaggtg gtcgaagggt gcgctgcccg
     3961 ccgggccgcg cagcagggtg gccaggtcgc cgctggccag cacgcgccca tgatcgagca
     4021 gcacgatgtg cgaacagacg gcgctcactt cctccatgta gtgggtcgca tagagcaccg
     4081 ccgtgccgtc ccgcaccatg tcgcggacgc gatcgagcag gaacgcacgg gattgcggat
     4141 cgacgccgac ggtgggctcg tcgagcagca gcacctcggg ttgctgcagc agggcgatgc
     4201 cgaggttgag ccggcgccgg acgccgcccg agcagtgccg cgctcggcgc ccggcaaact
     4261 cctgcagcat gcaggtctgg atcgcgccat ccactcgccg ctgcgcctgc gcgccggtca
     4321 gtgccagcat ggcggcgaag aaccggagat tctccaggca cgtgagctcc ggatagaagg
     4381 cgtactcctg gggcacccag gcgaccgacg gcacgcccgg ccccggatac cggatgcggc
     4441 cgccctgcgg cgtgcgcagc ccggccagca ggttgatcag cgttgacttg cccgagccgt
     4501 tgggcccgag caacccgacg gcctgcccgc gttcgatgct caggtcgacc ccgcaaagcg
     4561 cgggctccgc ctgcttgtcg tatcggtaga ccagctgttc cgcggcgatg acggccggcc
     4621 cgtcggtcat cgcaccgggg cgtccgtggt gcgagggatc cgctggggcg ggacgggggt
     4681 gctggccgcc gctgccgtcc ggccgggcag tacgggggca ggcttcaggc tgcgccacag
     4741 gcccagcggg gccatgccct ggcgcagcag ccgccccgcg accatcacga cgatcttggc
     4801 gatgtcgatg acgggccgga aatggctggc gcgcgcgtgc acggggtacc ggccggggat
     4861 ggccaccgcc agggtggggt gtccgcggcg ggcggcttcg atcaggatct cgctctcgaa
     4921 agtgaaccgg tcgccccgaa ccttcccgcc cagcgctatt tgcatcactt cgcggccata
     4981 aacgcgaaag cccgactggg agtcggcgat ggagtggccc gccgcccacg agatccaaaa
     5041 acacgcgaac cggttcgccc ggtagcgggc cggggggaac tgggagcggt cgtgcaggcg
     5101 cgagccgatc acgacgcgct cggggtgctg ccgccaggcc gccaggagcc cggccgcatc
     5161 cgcgggatcg tgctggccgt caccgtcgag ggcaatcacg caactcgccc gctgggcgag
     5221 cgcgtacgcg aacgcggtgc acaggctcgc cgccttgccg cggttctcgg ggtggaccag
     5281 cagcttgacg ggcaggtgct gcaactgggc ccgggtgccg tcgctggagc cgtcgtccac
     5341 cacgatcacg cgcgggcact gcaccagcgc ctcctggcac agcgcgcgta tcgtgccctc
     5401 ctcctggtag gccgggatgg cgatccacac gccgtcccat gacggcccgg cggattcttg
     5461 tcgtactgct tccatgctca gtaaaggcct ccgttgacac cgatgatctg cccggtgagg
     5521 tagctggccg cgtcggacgc gaggaaggcc accacggcgg caacttcctc aggccggcct
     5581 gcgcggcccg cggggaccat cgccttgatt ttctcgggcg caaacgcggc cgccatgtcg
     5641 gtgtcgatga tgcccggcgc cactgcgttg acggtgatgc cgcgcccggc cagttccagc
     5701 gccagcgatt tcgtcacgcc gtgcagtccg gccttggccg ccgcgtaatt gccctggccc
     5761 gggttgccgg cctgacccgc caccgaggtg atattgacga tccggcccca tcgcgtgcgc
     5821 accatcggca acagcagcgg ctgcgtcacc ctgaagaagc cattcaggtt ggtgtcgatc
     5881 acctcgtgcc acgcggccgg gctcatgccg gccagcaggg catcgcggtg cacaccggcg
     5941 ttgttgacga gaatctggat cggcccggcc gacagcacgg attcgaccgc ggcccgcacc
     6001 tgttcgtcat cggccagatc gaacgcgatg ggctccgccc ggccgcccgc ggccaccagt
     6061 gcggccacca gcgcctgcgc ccgttcgatg ccgcggttgg cgtgcacgat cacatgaacc
     6121 ccctgcgatg ccaaggcacg gcagatcgcc gcgccgatgc ccccgctgcc cccggtcacg
     6181 agggcgcggc gcaccgccgg ggctgccgac ccggtcatgg ccggcccacc ggcgcgaatg
     6241 ccaccatcag ccggccgctg gccacgggtg tgccgatgct ggccacgtcg aacgcgtaga
     6301 ggcaccccgc ggcggtgcgg ccgagcaact ccgcacgaac ggccagcggc ccgccgccgg
     6361 ccggaaagca ccggctgtgc aagcgcactt ccatcagctt ggcgagcatg ccggcccgcg
     6421 gcccgtgcgc gtcgtccagc aatgcgccat gcaccgcgct cgcttgcgcc gcgtactcgc
     6481 aagccgcgac agccggcacg cagccatccc gggcgagcgg atgccccgcc ccgggttcag
     6541 cggcgctgca agcgatatgg ccggcgtccc aggcggtgac cgcctccagc aggcacatcg
     6601 aacccgcgtg cggcaccatc cgctcgatgg cctcgcggtc gggcatcatg cacggccctc
     6661 ctgcgtgcgc tgcagggtca ggatctggcc gcccagggaa agccggcagg gtgtcccggc
     6721 ttcggcctcc agcagcccga gggccgccag tccgcgggcg ctgctgtggg cgacccatga
     6781 cgccggcaac cacgccggaa ggggcgttgc cgattgcgtc cccgccggct ccatcgcaat
     6841 gctgaaggcg cagagcaccg gcacggactc atcgaccggg ccgctgctga tgatccaagc
     6901 gctcgcggtc gcttcctgca cgggcagcag ttcgctcaac ggagatgtca tggccggatc
     6961 gtagaccacg agcagcacgg gctgggcggt ggactgcgcc tcggtgaccg cgcagagcag
     7021 cccactggcg aagctctcca ggccgagact caccacggtg gccggttgct ggttgcgcca
     7081 cgcgatcgaa aagtagccgg aaggcgcgtt gaggacggag ttgggaaaca gcaacgggga
     7141 cacctggcgc gtggtcgcca gcgtgtccag catctgctgg caaacctcgc cggtgccctc
     7201 gtcggtggcg aataccgatc gcatgtccct gaccgggaaa ggacaggtct ccagtgcctg
     7261 ttcgatgcag gccagcacga gccgcaccgg ttggctggcg cgccggcgct cgtgcgcggg
     7321 gagcagcgcc gggctgggga gtttcagcgg cgcgggcgtg tacggggcgg ccccgctgct
     7381 gacggctcgc agttccggca gcgatggcag gccgggcgcc aggacgccgg cggcgtggag
     7441 acggaaagaa agactcgtca tgtcactgcg ccagcgtgaa cacgagcgtg caattgctgc
     7501 cgccgaagcc gaagttgttg ctcatggcgt gttgcatgcg cgcggaggcg ggttgcctca
     7561 cgaggttcac cgcgagcgcc gggtccgccg tcatccagtt ggtgctgggc ggcagcgcct
     7621 ggtgctccag cgcctgcgcc gtcacgatcg cttccacgat gcccgcggcc ccgagggtgt
     7681 ggcccgtcgc gcccttggtg gaagacacag gcacggcatg cggcccaaac accgccgaca
     7741 gagccaccgc ctcggcgcga tcgttggccg gcgtggccgt gccgtgggcg ttgacgtatc
     7801 cgatgtcgga cggcccgatg ccggcggcgg acagcgccat tcgcatcgcc gcctgggctc
     7861 cttcgccttc gggatgcggt gtggacatat gccatgcatc gctggactcg ccccctcctg
     7921 cgaattgcac ggccgcaggc ctgcgctcca gcagcacgaa acccgccgcc tccccgatcg
     7981 agatgccgtt gcgtgcgaga tcgaagggcc ggcacatgtc gctggacagc agctgcagcg
     8041 cgtcgaagcc atgcaaggtc gtcaggcata ggctgtcggc gccgccgacg acggccgcgt
     8101 cgatcaagcc cgcgtcgatc catcgctgcg cggtgaggaa caccttggcg ctggaggaac
     8161 aggccgtcga aatggcggcc atcgggccct gcaagccaag caactgcgcg ctgaagcggc
     8221 agagcgcatc gaccgcgtgc gtcttgcggt agtcgaaggc ctcgggccac cggccggttt
     8281 cgcggcgatg cgcgtaggca gactccgtgc tgcggatgcc cgacgtgctg gtgcccagca
     8341 ccaggccgac gcgatgcgct ccatggcggc cgatcgcggc cgccagtgcg ctgcgaaacg
     8401 gcgcatcctg cagcgccagc caggcgaggc ggtggctgcg gcaatcccat gcgcccaggt
     8461 gcgccgacaa ggccgggtct tccacgccgg gcacggcacc gacccagcac gcgagctcgc
     8521 accagggcaa cgcgttgggt tgcaggccgg tgcggccacg ggagatggaa tcccaaagag
     8581 cccgcgtccc gtgaccggcc gggtgaacaa gggttgtggc ggaaattccg agcacgggtg
     8641 tccgatatga aaaactgcgc ggatcaggcc gccgggcggc actccagcaa ggtgtggtag
     8701 ccgccgacgc catcggtgac ggccgatact tcgagccccg cgcgctcgac gagcgccacg
     8761 aagacggaag aatcgtacat ctggctgttg ccattggcaa cgcaggtgaa gtaaagcgaa
     8821 gtctgttgca ggctgaatgc cgcggcctcg aagcgctggc ggtcccagaa cagctcgagg
     8881 atccacacgc gcccgcccgg cgcaagcact gcacgcgcct tgcgcaggat gccgacgatc
     8941 tgctcgtcgg cgaaacaatc gaggaattgg cacatccaga tcaagtcgta tccggccggc
     9001 agcgcggcgg cgggatcgag caagtcgacg ggatgaagcg tggcgcgctc gcccaggcca
     9061 gcatgggcca ggcgttcgcg cgcacgttcg agctgcaatg gcagatcgac cagccccacc
     9121 tccagcgccg gcaggcgcgt gaggcacacc tgcgcccacc ggcccgtatt gcaacccaca
     9181 tccagcagcc gttgtggcgg caaggatgcc agccgctcgc aggcggccgg aaaggcgccg
     9241 tccgaataga aatggtcgaa ggcgtgccag ctgcggcgcg agggctcggg catcaccgac
     9301 aggccttcgt acaacgtgtc ccacgggccg atctccttca ggcccaccgg acggccttgc
     9361 atcagtgact ggtcgaggtg ggccgcagcc cggtagcaga catcgcgggt gaagtcgaag
     9421 ttgacgcggg tcatttcgtc atcgagaagg aagcgtccca gcttgcccag gaagaagcgg
     9481 tcgccttgcc gccagacgat gtgcaggccc aggcccgcct ccagcaggac ccgcacggcg
     9541 tagaccgaca ggccggtcga tgccgccacc tcgtcggtcg ttcttccctg cacgggaccc
     9601 gccgtcgcga tcgccgcgag cacgccgcga tcgcgcatga cggccgtggc ctggaaggcg
     9661 agcggtgcga acgccagccg ctgggcggcg gtgacggctt ccagcgccgt gaggggatcc
     9721 ttgtcgtaca tcggtgcctt tgcggttgga agcgggatca gggctgcagg cgggtgatgg
     9781 tgcgttcgcc ctggcgttcg accacgatga tgacgtgcag ttgccccgcg gtgccctgca
     9841 gttccacgtg atcgagatgg cgggcgacgt ccgcgctgcg cggctggagc tgcgccgacc
     9901 agacgcgctc gtcgccggtg agcttgatct cgaaatcgcg ttgcagcgca accaggtccc
     9961 ccgcgaccac gcggcgcatc acctgcgaga gcgcggccag cgccggcgcg tgcgtgaaca
    10021 ggatttgctt gctcccggtt gcgcccggcc ccacccattc ggcgcgatcg gccagcagcc
    10081 gccaggtttc ctcgcgcggg gactccaccc gtttttcgag tgcggtcggc gtcgatcgca
    10141 tcgtgccgcg cgacgtcaag ggcgtcgaaa gccagggcga ttcgcgcact tcctgataac
    10201 tcacggacgg cctggcggcg gcttgcagcg cgcgctgcag ctgcgccacc gtgagcgccg
    10261 cggtttcggc catggcgatg ccaagacacg ccagcaccag catgaaaccg cccctgggaa
    10321 ggaaagcttt gatcatgccc tggcctcgcc ggccgcccag tacggataga aattgaacca
    10381 gttcaaagga tagcgtcgcg catactgttc gagcacggtc gcataccggt ccaccgcggg
    10441 ctgcagagcg gcgccgcgcg cctgccgggg agccgccggc ccgaactcca cgaactcgat
    10501 gcggtagcga ttgccgcctt cgtaaaggcc gaagcacatg agcacgcacg cctcgctgcg
    10561 ggcggccagc acgtggggcg caatgggcat ggtgaccctg cgccccagga acggcgcggc
    10621 aagcgctgtc gcggccccgt ccacccgatc ggcgaggatg ccgatgattt cgccgcgggt
    10681 gagcgcctca tgcgcgcgca ggtagctgtc ggggcggccc agcgggatca ggttcaacgc
    10741 ctgttcgtcg atgcccgcga tccggcgcag cctggcgcga gggtccatgc gcatcatcac
    10801 gctgacgggc cgtccatcca tcgcgcggtg ggcgagcatc atcaggtcga agctgccaag
    10861 gtgggagccc agcaacacac agccgcggcc cgcctccatc gcctggtcga ccagcgccag
    10921 cccctctatc gtcacctgga aacggtcgaa ctcaccggcc gccatgtaga cgcgatccag
    10981 caacgtcgcg gcgaagctgt agatgtgccc gaatacctcg ttctgccgga ccgggcgccc
    11041 atgaacggcg ctcaggaact cggtggatgc gcgccgcgcc ccggcgtcgg tcaggaagaa
    11101 atagaaggtg atggggtaga gcagcgcgcg gcacgccggc cggcccacgg ttcgcgccag
    11161 ccaggcgatc agctcgatca gccaggcgct gccgcgctcg gccttgtcgg accagccctt
    11221 catgccggcg cctccgccga caggatgccg acgccctcgg cgaccagcgg cgcggcgtcg
    11281 cctcgccggg cactgatgcg aaacgatacg cgctcgccgt ccatatccag caagacttgg
    11341 gcggcttcgc cgggacgcag ggccgatgcg aacttcacct gtttcagctg cacgagctca
    11401 cggccggtca gccggcaaag cgcctgctgg actcgatcga tcagcaatac gccgggcacc
    11461 accgggttcc ccggaaaatg cccctccagc gaaggatgat cgctgtcgac gcgcaggtcg
    11521 aactccagcc ggctcatcgc cccaccgcct tcggattctt ggccgcgtag atggcgtcga
    11581 gcgcggcgcg cgtcagtttg cccgcgtccg tgcgcgggag ccgctccacc tcgatgatgg
    11641 ttcgcggcag gaatgccggg tccatccgct cgcgcagcca ccggcgcgtg gccgcgcgat
    11701 ccagcggcgc gccggcgtga atcagcacca ggcgctccgt gggcgagccg gtcgccggca
    11761 ggtagaacac gccgtccacc aggccgggca agtcctgcag cagaagattg aggcccgcca
    11821 gcgaagcgcg tcttccggcg atcttgatca ggtcggcctg ccggcccagc agcctgaagc
    11881 cgccctcccc atcgagctcg acctggtcgg ccaggatccg gggcgatgcg aaatgcgttc
    11941 cccacacttg cgcgccgtcc ggcgccggct cgatgcgcac gccatcgacc ggccgccaat
    12001 gcgcttgccg cgccgtgcgg cgcatcgcca cgacgccggt ctcggtcgat ccatagatct
    12061 ccatcacggg cgcgcgtgcc aatgtctcgg cttgctcggc cagcccgggc gcgagcggca
    12121 tcgtcgagac cagcacgagt cggcatcggg gcacctcctg cccggatcgg gcgagcgcgc
    12181 tcaggtgcag cggcgttgcg atccaggcgc cctcgcccgg cgcgtcgcgg aaagccgcat
    12241 gcacatcggc ggcaagcaag ggccggcgat cgatgacggg aatcccggtg accaggggca
    12301 gcatcaccga cgtttcgacg ccgaacatgt gctgcggcgg cacgctgcag acgatctgcc
    12361 gcaacccggc ggtgccgcct tccacgacct catcgagccg ggcggcaagt accagcgcgc
    12421 cgcggcagaa ctcgcccagg gtcttggtct ggggttccgg cgcgcccgtg ctgccggacg
    12481 tgtaaagacg aatcgacggt gcatcccagt cgggcgacca ggccagcccg gcatccggcg
    12541 ccgaggcgga cggcacttgc gggcgatgga cgatgcaccg cgcatggtcc gaccagtgcg
    12601 gctgcagcag gttctcgtcg tcgaccacga tcaccgggct cgcgctggac tggaggatgg
    12661 ccgcgagatc ggtgtggccg ccggacggag gcagcaactg caggcagccg cgccgcagcg
    12721 aggccagcca cgcgacgagg aagcccacgc gcgagccgca caggttgcag acggtgttcg
    12781 cgccttcgag ccgccgcgcc agcgcaagcg atgccgcgtg cacctcacgc caggtccagc
    12841 catgctgcgc ggcgatcagc gccggctgcg gcaggtaacg gggtgcggcc gtgagcggcg
    12901 tgtcggcgag gtactgcatg gatggctcaa cctctgccgg gatgccagac gctccaggtg
    12961 tcgcgcagtt gctgcgtcag gcttgggaac acgcgtccgg gaaagaagaa tggacgcagc
    13021 cagtgttcgc caacgaagag cgccgccgtg cccgcccaca cccaggcgcc cgcgcgcaac
    13081 ggatactgcg ccgcggcgat ggacagcagg gcggcagcca cgaagtaggc acaccagacg
    13141 gccgtcagcc tgcgcgtgta acgccgcagc ggttgcgtca tttcggggtc gctcacggtg
    13201 gcgatgcgct cgatcagtgg gatggcgccc ggccgcaacg tgcggccgaa gaagtacgcg
    13261 agccagccca gcaccaggag ggacacggcg cgccacaggg tgagcgcatc cagcgactgc
    13321 agccagtcca ccggcggatc agcgttgcgc cgccaggttg cccgcaatat gggctgcgag
    13381 actttgcagt gaagcgaaga tggcctcgtt gtcgggatcg tccgccttca atttgacgcc
    13441 gtagcgctgc tggacgatca gcgatatctc gagtacgtcg agagagtcca ggtcgagccc
    13501 gccgccgaac agcggcgcaa gaaggtccac ctcgtcggga ttcacatcct cgaggttcag
    13561 cccctcgacc accaaggtgg caaggtccct tgcagacact tccgctctgg gctcgcccga
    13621 actcggtctc atgatgccga caactgggcg gtgatgccgc gggtcaggtt ctggatccag
    13681 ccgttgtagt tcttgtggat gttgccgccc ttttcgtcca ggttttcgct gctgcggtac
    13741 ttgatgctgt agctgctggc ggaatacgga atctcgacga cagcggtgtg cttgcgcagc
    13801 tggagcgtgc caaccagcat gctggggccc tcatccctca tctgccagcc cagcgcggcg
    13861 cctgcgcgga cgatcgcgcc ccgcacctgg tccttgctca gggccttgcc tgttgcattg
    13921 gtcgccggtg tttggctgac gttttgaatc ggcacggcag tgcagccggc aaccgcgatg
    13981 gcaactgcca atgcggcgag cgtgatgtga aaacgcatga acgaatctcc tccagggggt
    14041 gtgaacaggg tcggtggtct tgtattgtcg tgcccaccgc ccgcgatgca caggacgact
    14101 ctgagttctc gtggcgcccc gcgcaagcgg aattctactc aacaggaaga cgctcccaaa
    14161 ttagtcactg accccatgtc aaaaaacaac aatccgggcg cgggtgttcg cgagacctgg
    14221 tgatggtgga gtgaatggcg cggctgcgac gggaagaggc gcatcgtccg ggcactatca
    14281 ttcccgcatg gtcactctct atggcatccc caactgcgac acagtcaaga aagcccgcgc
    14341 ctggctcgcc gaacatgatg tggcctgcga attccacgac ttcaagaagc agggcgtgcc
    14401 cgttgcgcac ctcgatgcgt ggatcgccca gctcggctgg gagaagctgg tgaaccgaaa
    14461 gggcactatg tggcgcaggc tcgaaccggc aacgcaggca ggcgtgacgg acgcggccgg
    14521 cgcgaaggcg ctcatgctgg cgcaccccag cgtgatcaag cggccggtcg tggaatgggg
    14581 tgggaagacc acagtggggt tcgacgacgc gcagtgggcg ttgctcgcag gccgctagga
    14641 aggctccaaa gcccaacttt taccgtcgct ttacccgaaa agcggcggca acgccacggt
    14701 tgtgcctaga attttaggca tgagaacaag ccaaggagcc gtcatgaaaa agaccattct
    14761 gctgtccgcg atgggtgtgt tgtcggcctt cggttcggtg gcgggcgcgc aggaaatcgg
    14821 ccgggtgctc tccagcacgc ccgtgatcca gcaggtagcc gtgccacgcc aggtctgcaa
    14881 ctaccagccc atggccgtgc agcagcccaa ctccggcgcg ggcggcgtgg tgggcgcgct
    14941 ggcgggcgga gccatcggca accagatcgg ccacggcgac ggccgcgcgg ttgccaccat
    15001 cctgggcgtg gtcggcggcg cgttgctggg caaccagatc gagagcaaca acaacgcgcc
    15061 gcaggtgcag aacgtccaga gctgcaccac ccagacgtat tacgaaaacc gcacggtggg
    15121 ctacaacgtg ctctacgaat acgccggcaa gcaatacgac gttcagatgc ccaatgaccc
    15181 gggcccgacc atccggctgc agctcacgcc gatgggcgac agcggtgccg cccccgagat
    15241 ggccggctcc ttgtcacctg cgggctcgcc gggggtgatc gtggcaccgc ccatgacgca
    15301 ggccgcgggg cagccggtgg cccaggtggt agcggtgccg ccggcggcgt acccggcgta
    15361 ctatcccgcg tattcccccg cgtactaccc caggccctac tactacccgc ccatcgggct
    15421 gtcgctgaac ttcggttttt ccggcggcca ccggcatcat cgctggcgct gaaggcaagc
    15481 ttgtcatgcc ggacttgagg agcctgcccc ggaccccgga tcaggtccgg ggcaggcctg
    15541 atccgggggg catccatgac ttcctggatt gcgggtcaag cccgcaatga caactccctg
    15601 tccggggccg gatcgggaca agctttgcca gccatgacag acacggcgtt gcatgcaggg
    15661 ttgtcatgcc gggctcgacc cggcatccag tacggcgccg caacgcagtc cctggattgc
    15721 gagcccagcc cgcaatgaca acactcctcg ccgcccagct aaaattggtc gtttaccttc
    15781 cggcgttgcc gcaaccgcgg aacacccgct ccaatgacca cccaaaaaat cgacggcgcc
    15841 tgcatcacgg cgcaggccaa tgtgtacttc gatggcaaat gcgtgagcca cagcctcacg
    15901 ctggccgacg gctcgaaaaa gtccgtgggt gtggtgttgc cgtccgaact caccttcaac
    15961 accggcgcgc cggaaatcat ggaatgcgtc gcgggcggct gcgaatacaa gctggccgcc
    16021 agctccgact ggctgaaaag cggccccggc gagcgcttca gcgtccccgg caattccagc
    16081 ttccagatcc gggtgagcca agcctatcac tacatctgcc attttgggta attccgaggg
    16141 gttaggggct agggactagg gaaaaagcca acagtccaag ctcccaaacc aacactagcc
    16201 cctaatccct aacccctagc cctttctccc ctatgagcac catcctcgag catcttccca
    16261 ccggccagaa agtcggcatc gcgttttccg gcggcctgga caccagcgcc gcgttgcact
    16321 ggatgcgaca gaagggcgcg attccctacg cctacaccgc gaacctgggc cagcccgacg
    16381 agcccgacta cgacgacatt ccgcgcaagg cccttcagta cggcgccgaa aaagcgcggc
    16441 tgatcgactg ccggctgcaa ctcgcccacg aagggatcgc cgccctgcaa gccggcgcct
    16501 tccacatctc caccgcgggc gcgacttatt tcaacaccac gccgctcggc cgggccgtga
    16561 cgggcaccat gctggtggcg gcgatgaagg aagacgacgt ccacatctgg ggcgacggca
    16621 gcaccttcaa gggcaacgac atcgagcgct tctaccgcta cggactgctc accaacccca
    16681 gcctcaagat ctacaagccg tggctggacc aggcgttcat cgatgagctc ggcggccggg
    16741 ccgagatgtc ggccttcatg cagcagtccg gcttcgccta caagatgtcg gcggagaagg
    16801 cctattccac cgactccaac atgctgggcg ccacgcacga ggccaaagac ctggagcagc
    16861 tgagcaccgg gatcaagatc gtcaacccga tcatgggcgt ggcgttctgg aaggatgacg
    16921 tggcggtcaa ggcggaggag gtgcgggtgc gcttcgagga aggccagccc gtcgctctta
    16981 acggaatgac cttcgccgat cccgtggagc tgatcctaga ggccaaccgg atcggggggc
    17041 gccatggcct gggcatgagc gaccagatcg aaaaccgcat catcgaggcc aagagccgcg
    17101 gcatctacga agcgccgggc ctggccttgc tgtacatcgc ctatgagcgc ctggtgaccg
    17161 gcatccacaa cgaggacacg atcgagcagt accgcgacaa cggccgccgc ctgggccggc
    17221 tgctctacca gggccgctgg ttcgacccgc aggcgatcat gctgcgcgaa acggcgcagc
    17281 gctgggtggc gcgggcgatc accggcgaag tgacgctgga actgcgccgc ggcaacgact
    17341 attccctgct ggacacccaa tcgcccaacc tgacctacaa gcccgagcgc ctgaccatgg
    17401 aaaaaggcga atccagcttc tcgccgagag atcgcatcgg ccagctcacc atgcgcaacc
    17461 tcgacatcgt cgacacgcgc gagaagctgg gcgtgtacac caaggcgggg ttgctctccg
    17521 tcggcggcgg cgccgccttg ccaagcctgg gcgacggcca cagcgactga gctctcggcc
    17581 acggtcgcag agtctttcgg gcggcgcgcc aggcagaatg cacctgtcga cgcaagggtt
    17641 ttcctcctgc actaaacacg catttccgtg ctgagattga cagcgccgcc ggcgcccgtt
    17701 ccggcggtca ggaggtaatc catgaagcat tggcaagacc ggaccaacgc agttctgggg
    17761 ggctggctga ttgtcgcgcc gatggcgctg ggctttcaaa gcgatgtggg cgccctggcg
    17821 accaccatgg ccgttggctt gctgctcctc gccaccgccc tcggagcgat cttcgttccg
    17881 cgtgcctggg aggagtggac agaaaccgcc ttggggctgt ttctggcggt gtcgccgtgg
    17941 gtggtgggat tcagtacgcc gtcggcccgg gtgactgcgg tcgtgaccgg gctggtggcc
    18001 ctcaccctcg cgctgtgggc gctgcaggat agcggccagt ccaccgcagg cgaaagtacg
    18061 gcgcaataag gcgctgacac cggggcagga gcgcgccggc gcccgcggcc cgcggcggca
    18121 tttaatgccg ggctgcagcc gcaccggcag cgttgccccc cctggaacaa aatcccgtgt
    18181 tttcggctct aatcctcgcc gtcgagcgtg gtctgacttc gacggcaagg aggttgacca
    18241 tgaagcagat gaagcattgg caagaccccg tgatggcagt gctgggcgtt tggctgattc
    18301 tctcggcgtg ggtcctgggc tttcaagaca atttgaacgc cctggccaac gcggtgatca
    18361 tcggcatcct gctcatcgct gcggcgctgg gagcgatctt cgttcctcgc gcatgggaag
    18421 agtggaccga agtggtactg ggactgtggc tggccgtctc gccctgggtg cttggattca
    18481 gcgcgcaccc gaacgcaacg gtcgccacgg tagtcaccgg cctggcgatc atcgtcctgt
    18541 cgttgtgggt cctggcgacc gacaaggact ttgccggctg gcgccccgac cgtccggcgc
    18601 agtagcccac tgacgggccc cacacgtcgg gcgcctcccg cccgacgcgg gcacttccca
    18661 tcggcaggtt ttttggccgt ggtgggtacg gctagatcac caccggctcg ggctcgagcc
    18721 tgatgccgaa gcgttcgtaa acgctggtct ggatggcgcg cgcgagcgtt acgacctcgc
    18781 cgccggtggc gccgccccgg ttgaccagca ccagggcctg cttttcgtag acacccgcat
    18841 tgcccaccga cttgcctttc catccgcagg cgtcgatcag ccagccggcc gccagcttca
    18901 cgctgccgtc gggcatcggg tagtgcacga tgttcggctc gcgcgcgatg atgtcctggc
    18961 actgctcggg cgtgacagtg ggattcttga agaagctgcc ggcgttgccg atcacgctcg
    19021 ggtcgggcag cttggccttg cgcacggcgc acacccagtc gtagatctgc cgggccgggg
    19081 gggcgtggat gccggtctcc tggatcttgc gctgcaggtc cacataaccc agcaccggct
    19141 tccagggctt gggcaggcga aatcgcaccc gcaggatcag cgcccggccc cccaggccga
    19201 agtcgccctc cttggccgcg acatgcttga acacggaatc gcgatagccg aacgcgcatt
    19261 gcccggcgtc cagggtgtac gtcttgccgg tgaacaggtc catcgcatcc agcgattcga
    19321 aacggtcctg cagttccacg ccgtaagcgc ccacgttctg caccggggag gcccccaccg
    19381 tgcccgggat cagcgccagg ttttccagtc ccggatagcc ggtgtcgagc gtccacgtga
    19441 cgaaatcgtg ccagttctcc ccggccccgc cttccacaat cgtcgcgcgc gggccatcct
    19501 cgacgagccg gcgtcccatg acctcgactt tgagcaccag gggcttgacg tctccggtga
    19561 gcacgatgtt gctgcctccg cccaacacga atttggggat cgagccgtga tcggggctga
    19621 ccagcacctc ccgcacgtcc gcttcgccgc gtacccgcac cagggcgcgg gccttggcga
    19681 caatgccgaa actgttgtgc ggctgcaggg gtacgttttg ttccacgaac atgggagaat
    19741 tgtcgcatct gcacaaagca tcccggggga gtcacatgcc gtcgttcgac acagtctgcg
    19801 aagccaacat ggtggaggtg aagaacgcgg tggaaaacac cgccaaggag atcggcaccc
    19861 ggttcgattt caagggcact tcggccgcca tcgagctcaa ggacaaggac atcacggtgt
    19921 tcggcgatgc cgagttccag ctcacgcagg tcgaggacat cctgaccggc aagctcgcca
    19981 agcgcaatgt ggacgtgcgc ttcctcgaca agggcgacgt gcagaagatc ggcggcgaca
    20041 aggtcaagcg ggtggtcaag gtgcgtaacg gcatcgagag cgaccaggcc aggaagatcg
    20101 cccgcctcgt gaaggacagc aaactcaagg tgcaggccgc gatccagggc gaggcggtgc
    20161 gcatcaccgg cgccaagcgc gacgacctgc aggccgccat ggcgctgatc cgcaaggagg
    20221 tccccgacct gccgctgtcg ttcgacaact tccgcgatta aatgactccc ccccgaagca
    20281 gcctgcggct gcccagtaca gtcgccagag gcggcggctc ttcgggccgt ccaatcctgc
    20341 gcaggcaggc ttggagccgc ggccctcagc cccctcaagg ggacgccacc agcggaccgg
    20401 caaagccggt tccgcggtgg cccgcgattc gcttgatctg tttcgtggcg ctgttcgcca
    20461 gtgcgcctgc gggcgcgcaa tcggtagcgc tgcaaggcat gctcggcgcc aaggcgcttc
    20521 tgatcgtgga cggcaccgcg cccaggagcg tcgcgcccgg tgacactcac cagggcgtga
    20581 aggtcatttc cacctccggt gaccaggcgg tggtcgagat cgccggcaag cgccatacgt
    20641 tgcgcgtggg cgatgcaccc gccagcgttg gcgcccgcac cggtggcgac gcgcgcggca
    20701 gccgcatcgt tcttaccgcg ggcagcggtg ggcacttcac cacgcagggc gcgatcaacg
    20761 gccaggcggt gcagttcctg gtggacaccg gcgccacatc catctcgatg ggcgtgcccg
    20821 aagcgcagcg tatcggcctg aattacaagg ccggccaatt gggctggggc agcacggcca
    20881 acggcaacgt ggcggtctgg catgtcaaac tgggttcggt gcgcatcggc gacgtggtca
    20941 tcaacgacgt caacgccgcg gtgctgccct cggccatgcc ttatgtgctg ctgggcaaca
    21001 gcttcctgag ccgcttccag atgaagcgcg acaacgacca gatggtgctc gagcggcggt
    21061 actgaccgcc caacaccgat ccatgccgga ccacctcacc gacagcgtcg ccccgccctc
    21121 cgaagactcg cgctccgccc tcgcgccgct gcggcggccc gtgttccgca tgctatggct
    21181 cacatggatg gcggccaaca cctcgatgtg gatgaacgac gtggccgcgg cctggctcat
    21241 gacgtcgctg gcgccctccc cgctgtgggt ggcgctggtg cagtcggcgg caactttgcc
    21301 ggtattcctg ctgggcctgc ccagcggcgc gctggccgac atcctggatc gccggcgcta
    21361 cttcatgatg acgcagttgt gggtggccgc cgtcgcgtcg atcctgtgcg tcaccgtgat
    21421 gatgggctgg atgaccgcgc cgctgctgct ggcgctgacg ttcgccaacg gcgttggcct
    21481 ggcgatgcga tggcccgtgt tcgcc
//
LOCUS       DASWIY010000453         6257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1824045, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000453 DASWIY010000000
VERSION     DASWIY010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824045"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1082
                     /locus_tag="VGA59_16260"
     CDS             111..1082
                     /locus_tag="VGA59_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9721274.1"
                     /translation="MKRRLLLLSALAATAPFSSSCAIAQARPIRLIVPYAAGGPVDVT
                     ARLLAERVKDSLGTVIIENRPGGGGNIGADAVAKAAPDGLTIGISAVATHAINPWLFA
                     KMPYDASRDFEPITQMVRVPNVLVMNAETAKRLKINTLADLIAYGKANPARLNYGSGG
                     NGSAGHLAGEMFKQRAGIYAVHIPYNGGNPAQLALLSGQVDFNFDNLATAAPNIRAGK
                     LKALAVTTLARSQALPEVPPVADTLKGFAIDTWWGLVAPAGTPRDTVARLNHAFVAAL
                     QSPEAKTRFGALMAEPVTSSPDEFGKFMKAELAKYEKVVKLSGAKVD"
     gene            1289..1891
                     /locus_tag="VGA59_16265"
     CDS             1289..1891
                     /locus_tag="VGA59_16265"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX9721275.1"
                     /translation="MAALRTLLCLVFAAAAASLVLPVAAQTQPGSDIPRTSLLPAASR
                     SYLGLNLGRSNYNLPCGSIAFLCDDSDRSVQLYAGTMVGDFWGVELGYLNMGRIARAG
                     GETRAKLSLVGKAPLGRSFGVFGKVGTTWGRTETSVMGASGIAGGTERGFGLSYGAGV
                     SYSFTPRLSAVLEWDSNDFRFSGSGRDPVRRTSLGLQYRY"
     gene            complement(2146..2430)
                     /locus_tag="VGA59_16270"
     CDS             complement(2146..2430)
                     /locus_tag="VGA59_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9721276.1"
                     /translation="MDYFVYLMASGKNGTLYVGVTNDLVRRVSQHRSGAFEGFTDRYR
                     VHHLVWFESTPSIEAAIQKEKQIKNWKREWKIALIEKENAEWRDLYESLL"
     gene            complement(2686..3402)
                     /locus_tag="VGA59_16275"
     CDS             complement(2686..3402)
                     /locus_tag="VGA59_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9721277.1"
                     /translation="MSQTLLKVTGLKVAYGGIQAVKGIDFEVHEGELVTLIGSNGAGK
                     TTTMKAITGTQALNEGDIEYLGKSIKGKGAWDLVKQGLAMVPEGRGIFTRMTIGENLQ
                     MGAHIRTDKREIAADLEKVFTIFPRLKERKDQLAGTLSGGEQQMLAMGRGLMSRPKVL
                     LMDEPSMGLSPIMVDKIFEVVREVSGQGVTILLVEQNASRALQIADRAYVMESGLITM
                     SGGAKEMLSDPKVRAAYLGE"
     gene            complement(3399..4220)
                     /locus_tag="VGA59_16280"
     CDS             complement(3399..4220)
                     /locus_tag="VGA59_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9721278.1"
                     /translation="MADTVLRVAGISKRFGGLQALSDVGISIERGQVYGLIGPNGAGK
                     TTFFNVITGLYSPDSGTFELAGKPYVPSAVHEVAKAGIARTFQNIRLFAEMTALENVM
                     VGRHIRTHSGLLGAIFRTPSFKSEEAAIAKRAHELLEYVGIGKYADFKARTLSYGDQR
                     RLEIARALATDPQLIALDEPAAGMNATEKVMLRQLIDQIRKDNRTILLIEHDVKLVMG
                     LCDRVTVLDYGKQIAEGTPADVQRNEKVIEAYLGHNHVATGAAAPQVAATTGEKQ"
     gene            complement(4232..5374)
                     /locus_tag="VGA59_16285"
     CDS             complement(4232..5374)
                     /locus_tag="VGA59_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9721279.1"
                     /translation="MNFINRQNKYVIIAISAVLLLVLPLVLQQAGNAWVRIADAALLY
                     VLLALGLNIVVGYAGLLDLGYVAFYAIGAYMFGLLASPHLTETFEWMAAAFPEGLHMP
                     LWIVIPAGAALAGTFGILLGAPTLKLRGDYLAIVTLGFGEIIRVFLNNLDHPVNITNG
                     PKGLNQIDPIHFWGLNLGRPTEVLGFEIASVTMYYYLFLALVLVSVVICHRLELSRIG
                     RAWMAIREDEIAAKAMGINTRNLKLLAFGMGATFGGVSGAMFAAFQGFVSPESFALME
                     SIMIVAMVVLGGIGHLPGVILGAVLLSALPEVLRYVAGPLQAMTDGRLDAAILRQFLI
                     AVAMITIMLMRPRGLWPTPEHGKSLQTKPSDAVPVPSAASATSVPH"
     gene            complement(5391..>6257)
                     /locus_tag="VGA59_16290"
     CDS             complement(5391..>6257)
                     /locus_tag="VGA59_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9721280.1"
                     /translation="ALGYTMVYGIIGLINFAHGEVLMIGALTSWAIIVNAREAMPGIP
                     GWLMLLIALVISCVVTTALNFTIEKVAYRPLRNSPRLAPLITAIGMSILLQTIAMIIF
                     KPNYKPYPTLLPTEPFQIGGAVITTTQIMILGLTAISLATLMYLVNYTKLGRAMRATA
                     ENPRVAALMGVKPDTVISATFIIGAILATIAGVMYASNYGTVQHTMGFLPGLKAFTAA
                     VFGGIGNLAGAVVGGMLLGLIESIGSGYIGVLTGGVLGSHYSDIFAFIVLIIMLTLRP
                     SGLLGERVADRA"
BASE COUNT         1138 a         2017 c         1979 g         1123 t
ORIGIN      
        1 catggggcat attgtatagc gtaacgctac acgctgcagg gtgctggggt ttcctgcaag
       61 ggattgctgc ggcctggctg aaccggaacg aattctgcac aatgcgaccc atgaaacgca
      121 gactcctcct cctctccgcc ctggccgcaa cggcaccctt ttcatcctcg tgcgccatcg
      181 cgcaggcgcg gccgatccgg ctgatcgtgc cttatgccgc gggcggaccg gtcgatgtga
      241 cggccaggct gctcgccgaa cgcgtcaagg acagcctcgg aaccgtcatc atcgagaaca
      301 ggcccggcgg gggcggcaac atcggggcgg acgcggttgc caaggccgcg cccgatggcc
      361 tgaccatagg catctcggcc gtggccaccc atgcgatcaa cccctggctg ttcgcgaaga
      421 tgccgtacga cgcctcgagg gacttcgagc cgatcacgca gatggtccgc gtgcccaatg
      481 tgctggtgat gaacgccgag acggcgaagc ggctgaaaat caacaccctg gcggacctga
      541 tcgcctacgg caaggccaat cccgccaggc tcaattacgg cagcggcggc aacggcagcg
      601 cgggccacct ggccggcgag atgttcaagc agcgggcggg catctacgcc gtccatatcc
      661 cctacaacgg cggcaatccc gcgcagctgg ccctgctgtc gggacaggtc gatttcaact
      721 tcgacaatct ggccacggca gccccgaata tccgcgcggg caagctcaag gcgctggccg
      781 tcaccacgct ggcgcgttcg caggcactcc ccgaggtgcc gccggtggcg gacacgctca
      841 agggcttcgc catcgatacc tggtgggggc tggtggcacc ggccggcact ccgagagata
      901 cggtggccag gctcaaccat gcatttgtcg cagctctgca atcgcccgag gcgaaaaccc
      961 gcttcggcgc cctgatggcc gaaccggtga ccagcagtcc tgacgaattc ggcaaattca
     1021 tgaaggccga gctcgccaag tacgaaaaag tagtgaagct ttcgggcgcg aaagtcgact
     1081 gagaaaaagc gcttggggaa acccctagcc gcggtgcggc cggggaactt tgttgctgca
     1141 catcgcgtcg aatatggcgt atcagctacc aaaagcatag cgcgctgcag actgtcctac
     1201 gccgcctgtg cgacttgtcc gacacgccgt ccagttacca accggtacag tgactcttcc
     1261 aggacataca aaaccaggag ccaggagcat ggcagcgcta cgcacccttc tgtgtctcgt
     1321 tttcgccgcc gccgcggcgt cgctggtctt gcccgtggcc gcccagaccc agccgggctc
     1381 cgacattccc cgcacctcgt tgttgcctgc ggccagccgc agctacctgg gcctgaacct
     1441 gggccgttcc aattacaacc tcccctgcgg cagcatcgcc tttctgtgcg acgactccga
     1501 ccgttcggtg cagctttacg ccggaacgat ggtcggcgat ttctggggcg tggaactagg
     1561 ctatctcaac atggggcgca ttgcgcgcgc cggtggcgaa acccgggcca aactcagcct
     1621 ggtcggcaag gcaccgctgg gccgctcctt cggcgtgttt ggcaaggtag gcaccacctg
     1681 gggacgcacc gaaacctcgg tgatgggcgc cagcggcatt gccgggggca ccgagcgcgg
     1741 attcgggctg tcctatggcg cgggcgtcag ttactccttc acgccccggc tgtcggcggt
     1801 tctggaatgg gacagcaacg atttccggtt ttccggaagc gggcgcgatc cggtgcgccg
     1861 gacgagcctg gggttgcaat accgctactg attcacaaaa aaagctcagg gaaaccgaag
     1921 agcgaagacg cccgcgggcc gcaaggctcg cgggcgtgcc ttcttctcct gtcattgcgg
     1981 accccggatc cagtccgggg caggcccgat ccgcaatcca cttccccctg tcattgcggg
     2041 ccttctcctg tcattgcgga cctgatccgc aatccacgaa gtcatggacc ccggggcacg
     2101 cccgggatga cagccttctt ccgggatgac agccttcttc tcgtgtcaga gcagggactc
     2161 gtacaaatcg cgccactcag cattttcttt ttcgatgagg gcgatcttcc attcgcgctt
     2221 ccaattcttg atttgctttt ccttctggat ggcggcttcg atggacgggg tggactcgaa
     2281 ccagacgaga tgatgcacac gatagcgatc ggtgaaaccc tcgaacgcgc cgctgcggtg
     2341 ctgggaaacg cggcgaacca agtcgttcgt gacgccgaca tagagcgtgc catttttccc
     2401 gctggccatg aggtagacga aataatccat cgcatggatt gtcggaacag cctggattgc
     2461 gggtcaagcc cgcaatgaca attccttagg cgttgtcacg acaattcctt aggcgctgtc
     2521 acgacaattc cttaggcgtt gtcatgccgg accccggatc aagtccgggg caggcctgat
     2581 ccggcatcca tgtctttcct tgccggcgca caggcatgcg agcatggatt gcgggtcaag
     2641 cccgcaatga caactgaggc ccgcaatgac aactgagcaa gaccctcact cgcccaggta
     2701 ggcggcgcgg accttcgggt cgctgagcat ttccttcgcg ccgccggaca tggtgatgag
     2761 gcccgattcc atcacgtagg cacgatcggc gatctgcagg gcacggctgg cgttctgctc
     2821 caccagcagg atggtgacgc cctgcccgga gacctcgcgg accacctcga aaatcttgtc
     2881 gaccatgatc ggcgacaggc ccatcgacgg ctcgtccatc agcagcacct tcggccggct
     2941 catcagcccg cggcccatgg ccagcatctg ctgctcgccg ccggacaagg tgccggccaa
     3001 ctggtccttg cgctccttca gccgcggaaa aatcgtgaac accttctcca ggtcggcagc
     3061 gatctcgcgc ttgtcggtgc ggatgtgggc gcccatctgc aggttttcgc cgatggtcat
     3121 gcgcgtgaag atgccgcggc cctccggcac catggccaac ccctgcttga cgaggtccca
     3181 agcgcccttg cccttgatgc tcttgcccag gtactcgata tcgccttcgt tgagggcctg
     3241 ggtccccgtg atggccttca tggtggtggt cttgccggcg ccgttggagc cgatcagcgt
     3301 gaccagctcg ccctcgtgca cttcgaaatc gatgcctttg acggcctgga tgccgccata
     3361 ggccaccttc aggccggtga ccttcaacag ggtctggctc attgcttttc tccggtcgtc
     3421 gcggcaacct gcggggcagc ggcaccggtt gcgacatggt tgtgcccgag gtaagcctcg
     3481 atcaccttct cgttcctctg gacgtcggcc ggcgtgccct cggcgatctg cttgccgtag
     3541 tccagcaccg tcacgcggtc acacaggccc atcaccagct tcacgtcgtg ttcgatcagg
     3601 aggatggtgc ggttgtcctt gcggatctgg tcgatcagct ggcgcagcat gaccttttcg
     3661 gtggcgttca tgcccgcggc cggctcgtcc agcgcgatca gctgcgggtc ggtggccagg
     3721 gcccgcgcga tctccagccg tcgctggtcg ccgtaggaga gggtgcgcgc cttgaagtcc
     3781 gcgtacttgc cgatgccgac gtattccagc agttcgtgcg cgcgcttggc gatcgcggcc
     3841 tcttcggact tgaagctggg ggtgcggaag atcgcgccca gcaggccgga atgagtgcgg
     3901 atgtggcgcc ccaccatcac gttttccagc gccgtcatct cggcgaacag gcggatgttc
     3961 tggaaagtgc gggcgatgcc ggccttggcc acctcatgca ccgccgaggg cacgtaaggc
     4021 ttgccggcga gctcgaaggt gccgctgtcg ggggaataca ggccggtgat gacgttgaag
     4081 aaggtggtct tgccggcgcc gttggggccg atcagcccgt aaacctggcc gcgttcgata
     4141 ctgataccca catcgctgag ggcctgcagg ccgccgaagc gcttggagat gcctgcgacg
     4201 cgaagaactg tgtctgccat gcgtgcctcg atcagtgggg aacagaggtg gcgcttgcgg
     4261 cgctgggaac cggcaccgcg tcggatggtt tggtctgcag cgatttgccg tgttcgggcg
     4321 tcggccacaa gcctcgcggc cgcatcagca tgatggtgat catcgccacc gcgatcagga
     4381 actggcgcag gatggccgcg tccagccgcc cgtcggtcat ggcttgcagc gggccggcaa
     4441 catagcgcag cacctcgggc agggcagaca gcagcaccgc gcccaggatg acgcccggca
     4501 ggtggccgat gcctcccagc accaccatcg cgacgatcat gatcgattcc atcagggcga
     4561 acgactcggg cgagacgaac ccctggaacg ccgcgaacat cgcgccggac acgccgccga
     4621 acgtggcgcc catgccgaaa gccagcagct tcaggttgcg ggtgttgatg cccatggcct
     4681 tggccgcgat ttcgtcctcg cggatcgcca tccacgcccg cccgatgcgg gagagctcca
     4741 ggcgatggca gatcaccacg ctcacgagga ccagcgccag gaacaggtag tagtacatcg
     4801 tgaccgatgc gatctcgaag cccaggacct ccgtgggccg gcccaggttc aggccccaga
     4861 aatgaatcgg gtcgatctgg ttcaggccct tcggcccgtt ggtgatattg accggatggt
     4921 ccaggttgtt caggaatacg cggatgattt cgccgaagcc cagcgtcacg atggccaggt
     4981 aatcgccgcg cagcttgagc gtgggcgctc ccaacaggat gccgaaagtg cccgccagcg
     5041 ccgcccccgc gggaatcacg atccacagcg gcatgtgcag gccttccgga aacgccgctg
     5101 ccatccactc gaaagtttcc gtcaggtgcg gcgaggcgag gaggccgaac atgtaggcgc
     5161 cgatggcgta gaacgcgacg taccccaggt cgagcagccc ggcatagccc acgacgatgt
     5221 tcaggcccag tgccagcagc acatagagca gcgcggcgtc cgcgatgcgc acccacgcgt
     5281 taccggcctg ctgcaggaca agcggcagga ccagcagcag caccgcgctg atggcgatga
     5341 tgacgtattt gttttgcctg ttgatgaagt tcatcatggt gtgtacctcg tcacgcccgg
     5401 tccgccacgc gctcgcccag caggcccgac ggccgcagcg tcagcatgat gatcagcacg
     5461 atgaaggcga agatgtcgga gtagtggctg cccagcacac cgccggtgag cacgccgatg
     5521 tagccggacc cgatcgattc gatcagcccc agcagcatgc cgcccaccac cgcgccggcc
     5581 aggttgccga tgcccccgaa caccgccgcg gtgaaggcct tcaggcccgg caggaaaccc
     5641 atcgtgtgtt gcacggtgcc gtagttggac gcgtacatca cgccggcgat ggtcgccagt
     5701 atggcgccga tgatgaaggt ggcggagatc accgtatcgg gcttgacgcc catcagggcc
     5761 gccacgcgcg ggttctccgc tgtcgcccgc atcgcccgcc cgagtttggt gtagttgacg
     5821 agatacatca aagtcgccag cgaaatcgcc gtgaggccca ggatcatgat ctgggtggtg
     5881 gtgatcaccg cgccgccgat ctggaacggt tcggtcggca gcagcgtggg gtaaggcttg
     5941 taattcggct tgaagatgat catggcgatc gtctgcagca ggatcgacat gccgatggcg
     6001 gtgatcagcg gcgcaagcct gggactgttt cgcagcggcc ggtaggccac cttctcgatg
     6061 gtgaaattga gcgccgtggt gacgacacac gagatcacca gcgcgatcag cagcatcagc
     6121 cagcccggta tgccgggcat ggcttcgcgc gcattcacga tgatcgccca gctggtcaac
     6181 gcgccgatca tcaagacctc gccatgcgcg aagttgatca ggccgatgat gccgtacacc
     6241 atggtgtagc ccaacgc
//
LOCUS       DASWIY010000454        11252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3614342, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000454 DASWIY010000000
VERSION     DASWIY010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3614342"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VGA59_16295"
     CDS             complement(<1..472)
                     /locus_tag="VGA59_16295"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX9721281.1"
                     /translation="MNQPCLPIEPASPRPRRSRQARVAWGTRVVTIGGDAPVRVQSMT
                     NTDTQDAVGTAIQVKELAVAGSELVRITVNTPEAAAQVPYIREQLDRMGIDVPLIGDF
                     HYNGHRLLTDDPACAQALSKYRINPGNVGKGDKHDRQFGQMIEAAMRWGKPVRIG"
     gene            complement(500..1492)
                     /locus_tag="VGA59_16300"
     CDS             complement(500..1492)
                     /locus_tag="VGA59_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX9721282.1"
                     /translation="MSELGGREGAAGFAGPIAANDSLTAGALLRRAREAAGLHVAALA
                     VSLKVPVRKLEALEDDRWGELPDAAFVRALASSICRTLKIDPQPVLDRLPQTAAPRLI
                     RSGEGLNTTFRAPSDGAASNWLDQLSRPVFLVVFALLLGALILILLPYLPQDEFMAAV
                     TGSGPTAPAQPVTAPATVVEPSAPTGSLPAGNIAQPAPDGGAAPVSTPEAAPSPMALS
                     TVTPATVTPAVAVPAAAETAAEPDAADKPSAMAAGDIIVFRTSSASWIEVKDAKGVVA
                     VRRMMAAGDSVGASGALPLQVIIGRVNVTEVQVRGKPFDLRRVSRDNVARFEVK"
     gene            complement(1485..2315)
                     /gene="pilW"
                     /locus_tag="VGA59_16305"
     CDS             complement(1485..2315)
                     /gene="pilW"
                     /locus_tag="VGA59_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEX9721283.1"
                     /translation="MTRSAALALDRRERLHFWAALACTLLLALAGCASPSSDGSASRD
                     IVTESDEPEARKRARIRMELAVGYFEQGQTNVALDELKQVIAADPSFPDAYNLRGLIY
                     MRLNDLRQAEESFRRAAVLNPRDANVQHNYGWLLCQQRRYDESFRAFELAMANPLYAG
                     RAKTLMTQGLCQARAGRPEDAERSLARSYELDAGNPVTGYNLAALLYRRGDFTRAQFY
                     IRRLNNSELANAETLWLGVKVERRLNERVAMMQLAEQLKKRFPQSREATAYERGAFDE
                     "
     gene            complement(2322..3467)
                     /gene="rlmN"
                     /locus_tag="VGA59_16310"
     CDS             complement(2322..3467)
                     /gene="rlmN"
                     /locus_tag="VGA59_16310"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX9721284.1"
                     /translation="MTANLLDFDLEGLAAFCQELGEKRFRATQLFRWIHQRGAGDFDQ
                     MSDLAKQLREKLKTRARIEAPAVISRHESSDGTIKWLFDVGEGNAVEAVFIPEDDRGT
                     LCVSSQAGCAVGCRFCSTGHQGFSRNLTSGEIVGQLWFAEHFLRRHLGTGERVISNVV
                     MMGMGEPLQNYSALVPALRVMLDDHGYGLSRRRVTVSTSGMVRMIDRLARDCPVALAV
                     SLHAANDELRDNLVPLNRKHGLADLLDACNRYLEHAPRDFITFEYCMLDGVNDQPEHA
                     RQLVTLLKHHGGTGVSCKINLIPFNPFPQSGLARSPQKQVLAFAKILGDAGIVTTVRK
                     TRGDDIDAACGQLAGDVKDRTRVGDRIARQRIVMLKQVPALAPGAKE"
     gene            complement(3567..3992)
                     /gene="ndk"
                     /locus_tag="VGA59_16315"
     CDS             complement(3567..3992)
                     /gene="ndk"
                     /locus_tag="VGA59_16315"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX9721285.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGQIYARFEASGLKIVAARMAHLSR
                     REAEQFYAVHRERPFFKDLVEFMISAPVMIQVLEGENAILKNRDLMGATDPKKAAAGT
                     IRADFADSIDANAVHGSDAPETATVEVSFFFPAMNIHSR"
     gene            complement(4185..5651)
                     /locus_tag="VGA59_16320"
     CDS             complement(4185..5651)
                     /locus_tag="VGA59_16320"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX9721286.1"
                     /translation="MNEPLPGAVSLPADQEMDQKQTPAAVDTPVDSAAAERHEPDELW
                     DDRDGDIDDDDLDVERGAQPPGRAEPAVPISFADVVSGQFDAEVESAEAAPPKRVLSP
                     QAETPKLHKVLAQAGLGSRLEMEQLITQGRISVNNEPAHIGQRIQFGDQIKVNGKPIR
                     FRIDPPPARVIAYHKPVGEVVTNDDPQNRPTVFRKLPRLPQGKWQSVGRLDLNTEGLL
                     LFTSSGELANKLMHPRFGLEREYAVRVLGALSNEEKQRLLDGIDLEDGRAQFGAIEDG
                     GGEGANTWYRVTISEGRNREVRRLFEALGHAVSRLIRIRYGAMMLPRGLKRGAWMELD
                     ERDIRALMQAAGPNGSPRRAQGEGEGEPGGGGRGGRNRRRGNRNASRGNSAEGPPPRQ
                     GQGQPGQGQGGNAGGQGQQRRKDRGDRGDRAERQGGAQPDPMKTSFGYIGADSFTRQR
                     QGQGQGQGQGQGRRRGGGGGGGAGGGSGGGQRRGGRGR"
     gene            complement(5654..6343)
                     /gene="scpB"
                     /locus_tag="VGA59_16325"
     CDS             complement(5654..6343)
                     /gene="scpB"
                     /locus_tag="VGA59_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX9721287.1"
                     /translation="MNTTDAMRVLETALICSQQPLPVRELRVLFNDELGADTIKALLA
                     DLQNEWAQRGVELVHVASGWRFQSRPQMREYLDRLHPEKPPKYTRAVLETLAIIAYRQ
                     PVTRGDMEDIRGVTINALILKQLEDRGWVEVIGHRETAGRPALFATTRQFLDDLGLDS
                     LDQLPVPDSPAQQAQLLAALGTVADQPDLAIETPDGQNGQDPAEPATPVAGPQPAREG
                     AADDAAQAVET"
     gene            complement(6459..7424)
                     /locus_tag="VGA59_16330"
     CDS             complement(6459..7424)
                     /locus_tag="VGA59_16330"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX9721288.1"
                     /translation="MDAGPDAELRPFTIEAGQHGERLDRALAALVPEFSRSYLQQLIE
                     AGAVSLNAAVASRSAHKVKAGDRGEIELRPTPQSQAFRPEPMALAVVFEDEHLLVIDK
                     PAALVVHPAPGNWSGTLLNGLLARDSRASLLPRAGIVHRLDKDTSGLMVVARTRAVMD
                     ALVQRIAAREVVRQYVALAHRGWGGAAVRHVEAPIGRDPRNRLRMAVVEGGKSASTVF
                     ELLQNGPDGCWVRATLGTGRTHQIRVHMAHIGHPLVGDELYGGARAAGLARQALHAYR
                     LAFEHPVTGRPLEFHAPLPADLRQALGLWGLRYNGLEWLTSHAPG"
     gene            7511..8317
                     /locus_tag="VGA59_16335"
     CDS             7511..8317
                     /locus_tag="VGA59_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX9721289.1"
                     /translation="MLRAKLSALPVLAIGAAALLAACSSTPDDPTTGWSPNKIYAEAK
                     DELNSGSFDKAIPLFEKLEGRAAGTPLAQQAQLEKAYAQYKGGDQAQALSTLDRFVKL
                     HPSSPAIDYALYLKGIVNFNDNLGIFAFLSKQDLSERDQKAAKESFEAFKELVARFPD
                     SRYTPDARARMTYIVNSLAQYEVHVARYYYSRGAYVAAINRAQNALADYQDVPAVEEA
                     LFILYRSYEALGMTQLAGDTRRVMEKTYPQSEYLTRGFKSGPERPWWKLW"
     gene            complement(8277..10367)
                     /locus_tag="VGA59_16340"
     CDS             complement(8277..10367)
                     /locus_tag="VGA59_16340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX9721290.1"
                     /translation="MSAGAPPLGASALERGSAAGDLEGPVREAFAPGGVLSRAAEQFR
                     PRSGQTEMALAVARTIRAGGALVAEAGTGVGKTFSYLVPALLSGERVLLSTATKTLQD
                     QLFGRDLPRLVEALGLPVRTALLKGRSSYLCLHRMELARRDAALPDRASLRSLARIEQ
                     WAHVTRTGDLAELPGLDERSALIPLVTSTRENCLGAQCPRFRACHVNLARREALAADV
                     VVINHHLFFADLAVRESGMAELLPTVRVAIFDEAHQLNETGVQFLGAHLTTGQLLDFA
                     RDILGAGLQLARGLADWQQVASAVERSTRELRLVVGKNAPGARLRWVEEAPAQVNTQD
                     WRAALEQLHLACSQAMTALDTVSEIAPDFGRLFERADDLARRLWRFAEPCEPGAVRWV
                     DVGAQLRLVESPLDIAEAVQEKLLRAGEEEASRRAWIFTSATLGDDARLSWFTEPCGL
                     GQAQVLRVGSPFDYASQACVYVPRHLSKPGEPAHSGQVAALAAEGARRLRGRTMVLTT
                     TLRALRAIGEELQRLFEGRGEIEVLVQGQWPKRRLIERFREGDSQGRPGCMLVASASF
                     WEGVDVPGDALQLLIIDKLPFPPPGDPLVEARAQRLQAQGRSAFNDYFVPEAAVALKQ
                     GAGRLIRRESDQGVLVVCDTRLASMGYGRRLLAALPPMRTLGSNDEFLETLARLGAAA
                     TVTTTSTTGVPVRT"
     gene            complement(10370..10573)
                     /locus_tag="VGA59_16345"
     CDS             complement(10370..10573)
                     /locus_tag="VGA59_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEX9721291.1"
                     /translation="MDSNLHSPERRLIELRMEHADLDALIDRASEETPVDELMMRRLK
                     KRRLALRDQIARLELAIDPKEPA"
     gene            10689..>11252
                     /locus_tag="VGA59_16350"
     CDS             10689..>11252
                     /locus_tag="VGA59_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX9721292.1"
                     /translation="MAKGYRLSASTGIHKGDREYQQDQIALLSHARAAGCVLAIVADG
                     MGGRSGGRKASDQVMMTARQLFERYSPEHDDAPSLLKQMVHEAHIVIKLTAVSSEQEP
                     HSTLAAFLINPSGDCHWIHAGDSRIYHFHGGKLVKRTMDHSYVQTLVDRGELTEEEAN
                     VHPQSNILMGCLGAEDDPPAEPYFIPQL"
BASE COUNT         1733 a         3877 c         3808 g         1834 t
ORIGIN      
        1 cgccgatgcg cacgggcttg ccccagcgca tcgccgcttc gatcatctgg ccgaactggc
       61 ggtcgtgctt gtcgcccttg cccacgttgc ccgggttgat ccggtacttg gacaacgcct
      121 gcgcgcaggc ggggtcatcg gtgagcaggc gatggccgtt gtaatggaag tcgccgatca
      181 ggggaacatc gattcccatg cggtcgagct gctcgcggat atagggcacc tgcgcggcgg
      241 cctcgggcgt attgaccgtg atgcgcacca gctccgagcc ggccacggcc agttccttga
      301 cctggatcgc cgtgccgacc gcgtcctggg tgtcggtgtt ggtcatggac tgcacgcgca
      361 ccggcgcgtc gccgccgatg gttaccacgc gcgtacccca cgccacccgc gcctggcgcg
      421 agcggcgcgg ccggggcgat gcgggttcga tcggcaggca gggttggttc atttcacgcg
      481 ttcccgggca gggtggggct catttcacct cgaagcgcgc cacgttgtcg cgcgacaccc
      541 gccgcaggtc gaacggcttg ccccgtacct gcacttcggt gacattcacc cggccaatga
      601 tgacctgaag cggcagggcg ccggaggcgc cgacggagtc gccggccgcc atcatccgcc
      661 ggacggcgac cacgcctttg gcgtccttca cctcgatcca cgatgcgctg ctggtgcgaa
      721 acacgatgat gtcgccggca gccatcgccg agggcttgtc ggcagcgtcc ggttccgcgg
      781 cagtctcggc cgctgcgggc acggccacgg caggcgtcac cgtggcgggc gtgaccgtgg
      841 acaaggccat cggggagggt gccgcttcgg gtgtcgagac aggcgcggcg ccaccgtccg
      901 gggcaggttg cgcgatgttt cccgcaggca ggctgccggt gggggccgat ggttcgacca
      961 ccgtcgcggg cgcagtcaca ggctgcgccg gcgcggtcgg cccggaaccg gtgacggccg
     1021 ccatgaattc gtcttgcggc aaataaggca gcaggatcag gatcagcgcg ccgagcagca
     1081 atgcgaaaac gaccaggaac accggcctgg acaactggtc gagccagttc gaggcggcgc
     1141 cgtcgctcgg agcgcgaaag gtcgtgttca ggccctcccc cgaccggatc aggcgcggcg
     1201 cggcggtttg cggcaggcga tcgagcaccg gctgcgggtc gatcttgagc gtgcggcaaa
     1261 tgctggaggc cagcgcgcgc acgaacgcgg catcgggcaa ctcgccccag cgatcgtctt
     1321 ccagggcttc gagcttgcgc accggcacct tcagcgacac cgccagggcc gccacgtgca
     1381 ggcccgccgc ctcgcgcgcg cggcgcaaca gtgcacccgc tgtcagcgaa tcgttggccg
     1441 cgatcgggcc ggcgaatcca gccgcgcctt cccgtccccc gagctcactc atcgaaggcc
     1501 ccccgttcat acgcggtggc ctcgcgggac tgcggaaagc gctttttcaa ttgttcggcc
     1561 agctgcatca tggcgacccg ttcgttcagg cggcgctcca ccttgacacc caaccacagc
     1621 gtctcggcgt tggccagctc actgttgttc aagcgccgga tgtagaactg ggcacgagtg
     1681 aaatcgcccc gccggtacag caaggcggcc aggttgtagc cggtcaccgg gttgccggca
     1741 tccagctcgt aggagcgcgc gaggctgcgc tcggcatcct cggggcggcc ggcacgggcc
     1801 tggcacaggc cctgggtcat cagcgtcttg gcgcgcccgg cataaagcgg gttggccatc
     1861 gccagctcga acgcgcgaaa ggattcgtcg tagcggcgct gctggcacag cagccagccg
     1921 tagttgtgct gcacattggc gtcgcgcgga ttcagcacgg ccgcccggcg gaaactctcc
     1981 tcggcctggc gcaggtcgtt cagccgcatg tagatcagcc cgcgcaggtt gtatgcgtcc
     2041 gggaacgaag ggtccgcggc gatcacctgc ttgagttcat ccagcgccac gttggtctgg
     2101 ccctgttcga aatagccgac cgccagctcc atccggattc gggcccgctt gcgcgcctcg
     2161 ggctcgtcgg attcggtcac gatgtcgcgc gacgcgctcc cgtcggatga aggcgaagcg
     2221 catcccgcca gcgcgagcag cagcgtgcag gccagcgcgg cccagaagtg caggcgttcg
     2281 cgccggtcca gagccagagc ggcggacctt gtcatgtgtc attactcctt cgcacccggt
     2341 gccagggccg gcacctgttt cagcatcacg atgcgctgcc gggcgatgcg gtcgcccacg
     2401 cgggtgcggt ccttgacgtc gccggccagc tggccgcagg cggcgtcgat gtcgtcccct
     2461 cttgtcttgc gcacagtggt gacgatacca gcatcaccga gtattttcgc gaaggcgagc
     2521 acctgttttt ggggtgaacg cgccaggccc gactgcggga acgggttgaa ggggatcaga
     2581 ttgatcttgc acgacacgcc cgtgcctcca tggtgcttca gcagggtcac cagctggcgc
     2641 gcgtgctccg gctggtcgtt cacgccatcg agcatgcagt actcgaacgt gatgaaatcg
     2701 cgcggcgcgt gctccaggta gcggttgcag gcgtccagca ggtcggccag gccatgcttg
     2761 cggttcaggg gcacgaggtt gtcgcgcagt tcgtcgttgg cggcatgcaa cgacaccgcc
     2821 agcgccaccg ggcaatcccg agcgagccgg tcgatcatgc gcaccattcc cgaggtcgac
     2881 acggtgacac gccggcgcga gagtccatag ccatggtcgt ccagcatcac acgcagggcg
     2941 ggcaccaggg ccgaataatt ctgcagcggc tcgcccatgc ccatcatcac cacgttggag
     3001 atgacgcgtt cgccggtgcc caggtgccgc cgcaggaagt gctcggcaaa ccacagctgg
     3061 ccgacgatct cgccgctggt caggttgcgc gagaacccct ggtggccggt ggagcagaag
     3121 cggcagccca cggcgcagcc ggcctgggaa gagacgcaca gcgtgccgcg gtcgtcctcg
     3181 gggatgaaca cggcctcgac cgcgttgccc tcgccgacat cgaacagcca tttgatcgtg
     3241 ccgtcgctgg attcgtgccg ggaaataacc gccggggcct cgatgcgggc ccgggtcttg
     3301 agcttttcac gcagctgctt ggccaggtcg ctcatctggt cgaaatcgcc ggcgccccgc
     3361 tggtggatcc agcggaacag ctgggtggcg cggaagcgct tttcgcccag ttcctggcaa
     3421 aacgcggcca gaccctccag gtcgaagtcg agaagattgg ccgtcatcgc atggggagtc
     3481 ccgcccgacg cagcgccggg ccgccccaag cgcgtcgagg ccccctcggg gggcagcgac
     3541 gcgtagtgga gcgtgggggc ttcatgctag cgggaatgga tgttcatcgc cgggaagaag
     3601 aagctcactt ccaccgtggc ggtttcgggg gcgtcggagc cgtgcaccgc gttggcgtcg
     3661 atgctgtcgg caaaatcggc gcggatggtg ccggcggcgg ccttcttggg gtcggtagcg
     3721 cccatcaggt cgcggttctt caggatggcg ttctcaccct cgagcacctg gatcatcacc
     3781 ggggcggaga tcatgaactc gaccaggtcc ttgaagaagg ggcgctcccg gtggacggcg
     3841 tagaactgtt cggcctcgcg gcgcgacaaa tgggccatcc tggcggcgac gatcttcagg
     3901 ccggaggcct cgaagcgggc gtagatctgg ccgatcacgt tcttcgccac ggcgtcgggc
     3961 ttgatgatgg agagggtgcg ttcaatagcc attgttggat cttcctgact tgaagttatt
     4021 ggggtttcgc cgcagtcgaa agttcggcaa agccttggat tttagctggt cgcaatgaca
     4081 aatgactgcg cttcgcttca atgacaaatg gccgcaccgg cctgccgact tcgcctcgtc
     4141 atctgggacg cccggcgcag ccggcgtcat ttgtcatcgg gccgtcatcg gccgcggccg
     4201 ccgcgacgct gtccgccgcc tgagccgcca cccgcgccac cgccaccgcc gccgccgcgg
     4261 cgccggccct gcccttgccc ctgcccctgg ccctggcctt gccgctgtcg cgtgaagctg
     4321 tcggcaccga tgtagccgaa ggacgtcttc atggggtcgg gctgggcgcc accctgccgc
     4381 tcggcccggt caccgcggtc gccgcgatcc ttgcgccgct gctggccttg gccgccggcg
     4441 ttgccgccct gcccctggcc aggctggccc tggccctgcc gcgggggcgg gccctccgca
     4501 ctgttgccgc gcgacgcgtt gcggttgccg cgccggcggt tgcggccgcc ccggccaccg
     4561 ccgcccggct cgccttcacc ctcgccctgg gcccggcggg ggctcccgtt cggcccggcc
     4621 gcctgcatca gcgccctgat gtcgcgctcg tccagctcca tccaggcacc gcgcttgagc
     4681 ccgcgcggca gcatcatcgc gccatagcgg atgcggatca gccggctgac ggcatggccc
     4741 agcgcctcga acaggcgccg cacctcccgg ttgcggcctt cggaaatcgt cacccggtac
     4801 caggtgttcg cgccctcgcc gccgccgtcc tcgatcgcgc cgaactgcgc tcgcccgtct
     4861 tccaggtcga tgccgtccag caggcgctgc ttctcctcgt tgctcaacgc gccgaggact
     4921 cggacggcat actcgcgctc caggccgaaa cgcggatgca tgagcttgtt ggccagttcg
     4981 cccgagctcg tgaacagcag cagcccttcc gtgttgaggt ccagccggcc gaccgactgc
     5041 catttgccct ggggcagcct gggaagtttg cggaagacgg tgggccggtt ctgcgggtcg
     5101 tcgttcgtga ccacttcgcc caccggcttg tgataagcga tcacgcgtgc cggcggcggg
     5161 tcgatccgaa agcggatggg cttgccgttg accttgatct ggtcgccgaa ctggatgcgc
     5221 tggccgatat gggcgggctc gttgttgacg gagatacgcc cttgcgtgat gagctgttcc
     5281 atttcgaggc gcgaacccag cccggcctgt gccaacacct tgtgcagctt gggcgtctcg
     5341 gcctgcggcg agagcacgcg tttgggaggc gccgcctcgg cgctttcgac ctcggcgtcg
     5401 aactgcccgg aaacgacatc ggcgaagctg atgggaactg ccggctcggc cctgcccggt
     5461 ggttgggcac cgcgctcgac atccaggtcg tcgtcgtcga tgtcgccgtc gcggtcatcc
     5521 cagagctcgt ccggctcatg gcgttccgcc gcggccgaat ccactggggt gtcaaccgca
     5581 gccggagtct gtttttggtc catctcttga tcggcgggca gggaaaccgc gccaggcagg
     5641 ggctcgttca tgttcatgtc tcgacagctt gcgccgcgtc gtcggcggcg ccctcacggg
     5701 cgggttgggg gcctgcgacg ggggtggccg gttcggccgg gtcctggccg ttctgcccgt
     5761 cgggggtttc gatcgccagg tcgggctggt cggcgactgt gcccagcgca gcgagcagct
     5821 gcgcctgctg agccgggctg tccggcaccg gcaactggtc cagcgagtcc aggccgaggt
     5881 cgtccaggaa ctggcgggtg gtcgcgaaca gggcgggccg gcccgccgtc tcgcggtggc
     5941 cgatgacctc cacccagccg cggtcctcca gctgtttcag gatcagcgca ttgatcgtca
     6001 cgccgcggat gtcttccatg tcgccgcgcg tgaccggctg gcgataggcg atgatggcca
     6061 gcgtctcgag cacggcgcgg gtgtacttgg gcggcttttc cgggtgcagg cggtcgaggt
     6121 actcgcgcat ctgcggccgg ctctggaagc gccagccgct ggccacatgg accagttcga
     6181 ccccgcgctg ggcccattcg ttctgaaggt ccgcgagcag cgccttgatg gtgtcggcgc
     6241 ccagctcgtc gttgaacagg acacgcaact cgcgcaccgg caaaggctgc tgcgagcaaa
     6301 tcaaggcggt ttcgaggacg cgcatggcat ccgtcgtatt catggtcttg ccgttccggg
     6361 aaaaggcgcc atacggcgct caaatcaaag gctaaacagg ggcgatttca ccgctgcgcc
     6421 gcagtcaact caggttgcgc ggcgcatgcc accgcgggct agccgggggc gtggctcgtg
     6481 agccattcga ggccattgta acgcaggccc caaagcccga gggcttggcg caaatcagcc
     6541 ggtaacgggg cgtgaaactc gagcggccgg ccggtgacgg gatgctcgaa ggcgaggcgg
     6601 tacgcatgaa gggcctggcg cgccaatcca gcggcccgcg cgccgccata cagctcgtcg
     6661 ccaaccaatg gatgcccgat atgggccatg tggacgcgaa tctggtgcgt gcggccggtc
     6721 cccagggtgg cgcgcaccca gcagccatcc gggccgtttt gcagcagctc gaacacggtg
     6781 gatgcgctct tgccgccctc gaccacggcc atgcgcaacc ggttgcgcgg gtcacggccg
     6841 atcggcgctt cgacgtgccg caccgccgcg ccaccccagc cgcggtgcgc cagggcgacg
     6901 tattggcgca ccacctcgcg tgcggctatg cgctgcacca gcgcgtccat cacggcgcgc
     6961 gtgcgggcca ccaccatcag gccgctggtg tccttgtcca gccggtgcac gatccccgcc
     7021 cgcggcagca gcgatgccct gctgtcgcgc gcgagcaagc cgttgagcag tgtcccgctc
     7081 cagttgcccg gcgctggatg gaccaccaac gcggccggct tgtcgatcac cagcaggtgt
     7141 tcgtcctcga acaccaccgc gagcgccatc ggctcgggcc ggaaggcctg gctttgcggc
     7201 gtgggccgca gctcgatttc gccccggtcg ccggccttga ccttgtgcgc ggagcggctc
     7261 gccaccgctg cgttgagcga caccgcgccg gcttcgatca gctgctgcag gtagctgcgc
     7321 gagaactcgg gcaccagcgc ggccagcgcg cggtccaggc gctcgccgtg ctgcccggcc
     7381 tcgatcgtga agggcctcag ctcggcgtcc ggaccggcat ccagggcgtc ctcgagcccg
     7441 gcctgcgcct cgggccgcgg ggggtcggcc gatataatga atttgccctg tttcaagaaa
     7501 gttgcgttca atgcttcgag ccaaattatc ggcgctcccc gtcctggcga tcggcgccgc
     7561 ggcattgctg gctgcgtgct cgtcgacgcc cgacgacccc accacgggct ggagcccgaa
     7621 caagatttac gcggaagcca aagacgagct gaactccggc tccttcgaca aggccatccc
     7681 gctgttcgaa aagctcgagg gccgtgccgc gggcacgccg ctggcccagc aggcccagct
     7741 tgaaaaagcc tatgcgcaat acaagggcgg cgaccaggcg caggccctgt ccacactgga
     7801 ccgcttcgtc aagctgcatc cctccagccc ggcgatcgac tacgccctct atctcaaggg
     7861 catcgtgaac ttcaacgaca acctgggcat cttcgctttc ctgtccaagc aggacctgtc
     7921 cgagcgtgac cagaaggcgg ccaaggaatc cttcgaagcc ttcaaggagc tggtggcccg
     7981 cttccccgat tcacgctaca cgcccgacgc acgggcgcgc atgacctaca tcgtcaattc
     8041 gctggcccag tacgaagtgc acgtggcgcg ctactactac agccgcggcg cgtatgtggc
     8101 cgcgatcaac cgggcacaga acgcgctggc cgattaccag gacgtgccgg cggtggaaga
     8161 ggccctgttc atcctgtatc gctcctacga agcactgggg atgacccagt tggcgggcga
     8221 cacccgccgg gtgatggaga aaacctaccc gcaaagcgaa tacctgacgc gcggcttcaa
     8281 gtccggaccg gaacgcccgt ggtggaagtt gtggtaacgg tcgccgcggc gcccaggcgc
     8341 gccagcgttt ccaggaactc atcgttcgag ccaagcgtgc gcatcggcgg cagcgccgcc
     8401 agcagccgac ggccatagcc catgctggcc aggcgggtgt cgcacaccac cagcacgccc
     8461 tggtccgatt cgcgccggat caagcgtccc gcgccctgct tgagcgccac cgccgcttcg
     8521 ggcacgaagt aatcgttgaa ggcgctgcgc ccctgggcct gaaggcgctg cgcccgtgct
     8581 tcgaccagcg ggtcgcccgg cggcggaaac ggcagcttgt cgatgatcaa cagttgcaat
     8641 gcatcgccgg gcacgtcgac gccttcccag aaggaggccg aagccaccag catgcagccc
     8701 ggacggccct gtgaatcgcc ctcgcggaag cgctcgatca ggcggcgctt gggccactgc
     8761 ccctgtacca gcacctcgat ctcgccccgg ccctcgaaga ggcgctgcag ttcttcgccg
     8821 atggcccgca gcgcgcgcag cgtcgtcgtc aggaccatgg tgcggccgcg cagccgccgc
     8881 gccccctcgg ctgcgagggc tgcgacctgg ccgctgtgcg cgggttcgcc gggcttggac
     8941 aggtgccgcg gcacatagac gcaggcctgg ctggcgtagt cgaacgggct gcccacgcgc
     9001 agcacctgcg cttgccccag cccacagggc tcggtgaacc aggacaggcg cgcgtcgtcg
     9061 ccgagggtgg ccgaggtgaa gatccaggcg cgcctcgagg cttcttcctc gccggcgcga
     9121 agcaatttct cctggacggc ctcggcgata tccagcggcg actccaccag ccgcaactgc
     9181 gcccccacgt cgacccagcg caccgccccc ggctcgcagg gttcggcaaa gcgccacagc
     9241 cgccgcgcca ggtcgtcggc gcgttcgaaa agacgcccga agtcgggcgc gatttcgctc
     9301 acggtgtcca gtgccgtcat cgcctgcgag caggcgaggt gcaactgttc cagcgccgcg
     9361 cgccagtcct gcgtgttcac ctgggcgggc gcctcctcca cccagcgcag cctggcgccc
     9421 ggcgcgttct ttcccaccac cagccgcagc tcgcgggtgc tgcgctccac cgccgaggca
     9481 acctgctgcc agtccgccag accgcgggcc agctgcaggc cggcgcccag gatgtcgcgc
     9541 gcgaaatcca ggagctggcc cgtcgtgaga tgcgcgccga ggaactgcac gccggtttca
     9601 ttcagctggt gggcttcgtc gaagatcgcc acccggaccg tgggcagcaa ttcggccatg
     9661 cccgattcgc gcaccgcgag atcggcgaaa aagagatggt ggttgatcac gaccacgtcg
     9721 gccgcgaggg cttcgcgccg ggccaggttg acgtggcagg cgcgaaacct ggggcattgc
     9781 gcgcccaggc aattttcgcg cgtggacgtg accagcggta tcagagccga ccgctcgtcc
     9841 aggccgggca attcggccag gtcgccggta cgcgtgacgt gcgcccattg ctcgatcctg
     9901 gccaggctgc gcaaggacgc gcggtcgggc aaggcagcgt cgcgccgcgc cagttccatc
     9961 cggtgcaggc acagatagct ggagcgtccc ttgagcaagg cggtgcgcac tggcaacccc
    10021 agcgcctcca ccagccgcgg caggtcgcgg ccgaacagct ggtcctgcag ggtcttggtc
    10081 gcggtggaca gcagcacgcg ctcgccgctc aggagggccg ggaccaggta agagaaagtc
    10141 ttgcccaccc ccgtgccggc ttccgccacc agggccccgc cggcccggat ggtgcgcgcc
    10201 acggccagcg ccatctccgt ctggccgctg cgcgggcgaa actgctcggc ggcgcgcgac
    10261 agcacgcccc cgggcgcgaa tgcttcccgg accggccctt ccaggtcgcc cgctgcgcta
    10321 ccgcgctcga gggcgctggc gcccagcggc ggcgcgccgg cgctcatcat caggcgggtt
    10381 ctttggggtc gatcgcgagt tccaggcgcg cgatctggtc gcgcagtgcc aggcgccgct
    10441 tcttgagccg gcgcatcatc agctcgtcga ccggcgtttc ctcgctggca cggtctatca
    10501 gggcgtccag atcggcatgc tccatgcgca attcgatcag acggcgttca ggggagtgga
    10561 ggttggaatc caaggaatct gccggtttcg ggtcgggcca aggtcgggtt cgttcaataa
    10621 tacgtgtttc gggggcaata atcgagcgct cgcccccatg attgtggggc gccggaaaat
    10681 cattcagcat ggccaaaggt taccgactca gcgcatcgac cggcatccac aagggtgacc
    10741 gcgagtacca gcaggaccag atcgccctgc tcagccacgc ccgcgcggcg ggctgcgtcc
    10801 tggcgatagt ggccgacggc atgggcggcc gcagcggcgg gcgcaaggcg tccgaccagg
    10861 tcatgatgac cgccaggcag ctgttcgagc gctattcccc cgagcatgac gacgcgccct
    10921 ctctgctcaa gcagatggtg cacgaagcgc acatcgtcat caagctcacg gcggtctcct
    10981 ccgagcagga gccgcacagc acgctggcgg ccttcctcat caacccatcg ggcgactgcc
    11041 actggatcca tgccggcgac tctcgcatct accatttcca cggcggcaag ctggtcaagc
    11101 gcacgatgga ccactcctac gtgcagaccc tggtggaccg gggcgagctg acggaagagg
    11161 aagccaatgt ccacccgcaa tcgaatatcc tgatgggttg cctgggagcc gaggacgacc
    11221 cgcccgccga gccatatttc attccgcagt tg
//
LOCUS       DASWIY010000455         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_685810, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000455 DASWIY010000000
VERSION     DASWIY010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685810"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VGA59_16355"
     CDS             complement(<1..740)
                     /locus_tag="VGA59_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX9721293.1"
                     /translation="MTLLDITLLSPLIGSLLLAIIGHRPIGGWINVVVGAVTFATSLA
                     MALDVFAHGPTLSSGRMFYIDAFNVYLVALTAFVGFTTSIFSRPYMRHEVERGRLTDG
                     RLRLYHSMYQGFQFAMLLALSTNNLGVLWVAMEAATLTTVLLVSLYRTPESIEAAWKY
                     FILCGVGIAQALFGTVLVYFAAEQKLGAARDDTLLWTVLHANAAGLDPTVLSLAFVFL
                     LVGYGTKVGLVPLHAWLPDAHSEGPTPMS"
     gene            complement(772..1494)
                     /locus_tag="VGA59_16360"
     CDS             complement(772..1494)
                     /locus_tag="VGA59_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HEX9721294.1"
                     /translation="MTELSQLSLYNQAIILFAALVLFTSFIMLAQARMLPLIFAFAWQ
                     GMLLAVVTALVALVSGHPHLYISAALTVALKVFLIPWLLHRLAVKLNIHLEAEAIKYP
                     SLLLLAGAGLVIFSYYVALPIVHLSGLATRNTIAVSIAVVLLGMLLMIARKKAVAQVI
                     GFMSIENGLFFSAVVSTYGMPLIVELGVAFDVLVAAILFGVFFFHMSESFDTLDVDRL
                     NRLSEVHETLFQPEEGSEEERP"
     gene            complement(1496..>1755)
                     /locus_tag="VGA59_16365"
     CDS             complement(1496..>1755)
                     /locus_tag="VGA59_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HEX9721295.1"
                     /translation="KLMIYAVLIANIFWPWGIAQEFTSEELGIGVVAIAAKLMALAVV
                     LVVWETVLAKMRIFRVPPFLGFAFLLSLLGMLTHVVLETGG"
BASE COUNT          395 a          548 c          553 g          259 t
ORIGIN      
        1 gacatcggcg tcgggccctc ggagtgcgcg tccggcagcc acgcgtgcag cggcaccagg
       61 ccgaccttgg tgccatagcc aaccagcagg aacacgaacg cgagcgacag caccgtgggg
      121 tcgaggccgg cggcgttggc gtgcagcacc gtccacaaca gcgtgtcgtc gcgcgcggcg
      181 ccaagtttct gctcggcggc gaagtacacg agcacggtgc cgaacagcgc ctgcgcgatg
      241 cccacgccgc aaagaataaa gtatttccac gcggcttcga tggattcggg cgtgcggtag
      301 agcgacacca gcagcaccgt ggtcagcgtg gcggcttcca tcgccaccca caggacaccg
      361 agattgttgg tcgagagcgc cagcagcatc gcgaactgga agccctggta catcgagtga
      421 tacaggcgca ggcgtccatc ggtaagacgc ccgcgttcga cctcgtgccg catgtacgga
      481 cgcgagaaga tcgacgtggt gaagccgacg aaggcggtga gcgcgaccag gtagacgttg
      541 aaggcgtcta tgtagaacat cctgccgctc gacagcgtcg gaccgtgcgc gaatacgtcg
      601 agcgccatgg cgagcgaagt ggcaaaggtg accgcgccca cgacgacatt gatccagccg
      661 ccgatcgggc gatgcccgat aatggcgagc agcaggctgc cgatgagcgg cgagagcagc
      721 gtgatatcga gcaacgtcat caatgaagcc ctgcttcgtt cgtcttgata ttcatggccg
      781 ctcttcctcg ctgccttcct cgggctggaa caaggtctca tgcacctcgg acaggcggtt
      841 caggcgatcc acgtcgagcg tgtcgaacga ctcactcatg tgaaagaaga acacgccaaa
      901 caggatcgcc gccaccagca cgtcgaaggc cacgcccagc tcgacgatga gcggcatgcc
      961 gtaggtcgaa accacggccg agaagaacag cccgttctca atcgacatga agccgatcac
     1021 ctgggccacc gctttcttgc gcgcgatcat gagcagcatt ccgagcaaca ccacggcgat
     1081 gctgaccgcg atggtgttgc gcgtggcgag ccccgaaagg tgcacgatcg gcagcgccac
     1141 gtagtagctg aaaatcacca gccccgcgcc cgcgagcagc agcagcgacg ggtacttgat
     1201 cgcctcggct tcgagatgaa tgttgagttt taccgccagc cggtgcagca gccaggggat
     1261 caggaacacc ttgagtgcca ccgtgagcgc cgccgagatg tagaggtgcg ggtgcccgga
     1321 aaccagggcg acgagcgccg tcaccaccgc gagcaacatg ccctgccagg cgaaggcgaa
     1381 gatcaacggc agcatgcgcg cctgggcaag catgatgaac gaggtaaaca acaccaacgc
     1441 ggcgaacagg atgatcgcct ggttgtaaag actcaactgg gaaagttcgg tcaccctagc
     1501 cccctgtctc cagcaccacg tgtgtcagca ttccgagcag gctcagcagg aacgcaaacc
     1561 cgaggaacgg cggcacgcgg aagatgcgca tcttggcaag caccgtctcc cacacgacca
     1621 gaaccactgc gagcgccatg agtttggccg cgatggcgac gaccccgatg cccagttcct
     1681 cggacgtgaa ttcctgcgcg atgccccacg gccagaagat gttggcgata agcaccgcgt
     1741 agatcatgag cttga
//
LOCUS       DASWIY010000456        10562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3615902, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000456 DASWIY010000000
VERSION     DASWIY010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10562
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3615902"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VGA59_16370"
     CDS             complement(<1..636)
                     /locus_tag="VGA59_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721296.1"
                     /translation="MRMHIRPTRAIGLLATLTIVLIAVSVIFLLLDMRKRELETARLD
                     TIGFAHMIREETEHTFDRVDLVLRGVQERMQTTYGSQLDLDSVSVRLLMSARIFGIRQ
                     LAVLYLLDADGTLVNSSRDGDDLGTSHAGEEYYKAFASGRAEGLYIGKPVRDRGHGGW
                     TLHIARSFAGTDGKFRGVTVATMDLSQLEQVYRAMKADHPRAMSLYLADGTL"
     gene            888..2918
                     /locus_tag="VGA59_16375"
     CDS             888..2918
                     /locus_tag="VGA59_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX9721297.1"
                     /translation="MHIEKRSLSRLRRFAVACWLVASACLGVSGAGAQTADGAAAVKL
                     DNDTCLTCHDAKKPKIESRDAAGKARALHAIPSDKFEQSVHSMMQCVSCHSDIKDNAQ
                     KGNAHAKDTAEPLKKVDCANCHQELWEQTQKRGRAADRPRLGVVAKNLEAYKKSFHAR
                     PNADDKTKPNATCDNCHDVHTFNVPPRNTPEHTQWRLGIPGMCGTCHADQLESYDKSV
                     HAQVNSNKLLADAAVCSDCHTAHAVTGTSGSPFKLTVTANCGNCHKQEYDSYKDTFHG
                     KITTLGYSYTAKCYDCHGSHEIARIASTESPANPANRLKTCQSCHNPEKGLPDVSAGF
                     ASFQPHGNVHDVSKYPQMWFAFQMMVGLLVGTFAFFWLHTALWFYREYKDRQARGGGE
                     AHVRLDEVPAPLRGKHVQRFNRTWRIAHLTFALSLMVLTLTGMPLFYSEVTWAPAIMN
                     ALGGPQVAGIIHRTAAVIFAGVFLWHLVYMMFTLGRKWRTFKLFGPNSMVPSLQDGKD
                     MLAMFKWFFGKGPRPVLDRWTYWEKFDYWAPFWGVTIIGVSGLVMWLPGFFGSFLPGW
                     VFNVAAIFHGEEAFLAVVFLFTVHFFNNHFRPDKFPVEVVMFTGTMSLEEFKKDHGVE
                     YQRLVDSGELERHLVEPPSTFMVKGSKLLAFVLIIVGLTLLTLVGVGFFTGG"
     gene            3048..3377
                     /locus_tag="VGA59_16380"
     CDS             3048..3377
                     /locus_tag="VGA59_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9721298.1"
                     /translation="MKLITTLAVACGLMTASIANAAVDAAAAQASVKESGCLACHAID
                     KTKAATSFKDVAKKYKGKADAQDKLVKHVTLKPMVEKDGEKMPHKALKNTAPAAVTNV
                     VQWILSL"
     gene            3660..4757
                     /locus_tag="VGA59_16385"
     CDS             3660..4757
                     /locus_tag="VGA59_16385"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9721299.1"
                     /translation="MFATPQDIAEGKRVALASCTSCHAVTGIALARGTPHIAGQRPAY
                     LYAELRVYQAGRRGNNPMHNVVKFLSDDALVKVAAYYASLDPAPPAPAAAKKAASKAD
                     PVAAGEAAASGCSGCHGDNGISETPGMPNLVGLDAKYIADATVAYKNGQRKHGMMKTL
                     VSALSEAEIRNISVFYALQKPAKAKTPAEGNKEAGKAAAAACAGCHGQGGVSTGTAPS
                     LAGQDAQYFATAMNAYKTGARADAAMKAPAASLDDKVIKDLAAYYADQAPQPPRVKKP
                     MTTEEIADRCNRCHGVDGNSTDPRTPAIAAQRVDYLEPVMRAYRKGERKSTAMTAMLD
                     GMSDEDIAELAVHYASKKARSVVYVPVPTKP"
     gene            4767..5621
                     /locus_tag="VGA59_16390"
     CDS             4767..5621
                     /locus_tag="VGA59_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721300.1"
                     /translation="MSTPPIALHPSAQPEESEKSEKSEKSLMSQPQLHRLYNHYSELP
                     FSMRILYTCVLCVVGMGYLFAFIYLFHTYSGRDGQAGLSYDDLVIAYTGSGKGSRLES
                     ALRGPMSTMLPADEATALISWAQQGGERATYDTFVAPLLDKRCVSCHDGSNPHLANLG
                     GYDNVKKVTEQDKGTDIFTLVRVSHIHLFGIVIFLYVVGTIFGHAYVRPLWVKYVAMA
                     APFVALMIDVSSWYLVKLYHPFAWLTMAGGAMLGLSFAFMWVISMYQMWIGKTPKAVL
                     ERGSEAIG"
     gene            5707..6828
                     /locus_tag="VGA59_16395"
     CDS             5707..6828
                     /locus_tag="VGA59_16395"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9721301.1"
                     /translation="MKNVLAGIVLTLLALAACTQKDEPAPKPVATDIAAGKVIAEREC
                     KACHGANGGGVAPAIPHLAAQRESYLMASLNEYKDGKRSHAALKDLTKQLSEADLRNV
                     AAYFASLPPVAHPTAQDVKHSSPYEKGKALAAACAGCHGEDGNATTPGTPSLAGQQPH
                     YLVAAIQEYHQGDRAGSMRSQLRDADRIELENLALYFAAQTPAQRAPASRGDPVAGEP
                     LTAMCGGCHGARGVSSDAATPSLAGQDAQYLVDATKAYRKTRQNWGMQRYVSGLSDKD
                     IDNIAAYYVAQKPRAADQVPSSTQDLAAKCDRCHDAEGNASAPAPKMRGQDKDYLIMA
                     MRAYRDDKRESSTMHRMSGAYSNAIIESLASWYSSQPPK"
     gene            complement(6884..7534)
                     /locus_tag="VGA59_16400"
     CDS             complement(6884..7534)
                     /locus_tag="VGA59_16400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9721302.1"
                     /translation="MAESIRILVADDHPLFREGVVHSLASEPDIAVVGQAASGEEALR
                     MARDLLPDVVLLDIGMQGWDGIVTAEKIRTACPATRIMMLTVMEDEDKLLASFKAGAR
                     AYVLKGVSAHDLARVVRSVAQGEVYVTPSLAAGILVTLSQGQAPQDPLGELTERESEI
                     LNLVVLGLTNREIGDRLHLSEKTIKHYMTNILEKLHVRSRVEAAMLAVRGARRHEG"
     gene            complement(7527..8942)
                     /locus_tag="VGA59_16405"
     CDS             complement(7527..8942)
                     /locus_tag="VGA59_16405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX9721303.1"
                     /translation="MKAISRLSLVHRFLLVGFLVLVATMIFIGTWVGSQIQSGIMNGM
                     GVTTDLYLDGAISPHLQSLADGRPLEAADRIALQRKLASTHLGERIVVLKIWGPEGRV
                     IFDSSDPSLATTVPDLARRPPASAFAGQVSSRIVEPSELESVFKERKRSRLIQTYSPI
                     RADSDRSVIAVADFYQAADGMEQAAREAQLRSWSVHGAVTLAMLALLATLVRRATNTI
                     AAQQRELGEKVDQLTALLAQNEQLHDRVRRAGARTTALNERFLRHVATDLHDGPGQGL
                     ALASMRIESLAEVCSACAQGSGHDRSIADEFHTIHVALQSALTDMRAILRGLRLPEIE
                     QLSVADTARRAVRDHEHKTGLAVPLSIGNVPDEAPLSVRIALFRLLQESLANGYRHGG
                     TSSQRVSLEVVDAQLIVEVSDEGKGFDPKASIDGGHLGLQGMRERVELLGGRFDVESA
                     AERGTVVRASLPLAQQAHEYG"
     gene            9217..10020
                     /locus_tag="VGA59_16410"
     CDS             9217..10020
                     /locus_tag="VGA59_16410"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9721304.1"
                     /translation="MRRRRFAVMTLLATVSLAPLCGAAQLAPASKSKQSVFQATLETR
                     PQSTPELSTDDFKAFLAGKSGVVLDARPKLEFELAHIPGSISIEATGLLRIVQELPRP
                     TTAIVIYSDGPFSERTKLRAEELLSMGYTNVSRYQLGLAVWRALGNTAATSLEGLRRI
                     FHGNIAVFVDARSRAEYAAGTIPAAETVLAGEVGNARRDHRLQYHDHATRIVVFGNSA
                     LEARTVAEEIARNAYSNSSFFSGTYQELKQAKFFSERKPSPLNLDGVTR"
     gene            10036..>10562
                     /locus_tag="VGA59_16415"
     CDS             10036..>10562
                     /locus_tag="VGA59_16415"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX9721305.1"
                     /translation="MPKHIFRLLCLLFGVLVLAFIAAKVMTADSFYKFGHYRADSVKE
                     IAAHEPVYQAANYCLSCHVKRVAQWSANSHKSVTCEVCHGAALGHPDNGKLPIPKDTA
                     KLCTLCHEAMPGRPHTQPQIVLAQHNPVATTGQQCTACHNPHSPKITAGLVKVTGNAA
                     AGKQRAADCADCHGE"
BASE COUNT         2063 a         3422 c         3287 g         1790 t
ORIGIN      
        1 cagcgtgccg tcggccaggt acagcgacat cgccctcggg tggtcggcct tcatggcccg
       61 gtacacctgc tccagctgtg aaaggtccat cgtcgccacg gtcaccccgc ggaacttgcc
      121 gtccgtgccg gcgaaggaac gggcaatgtg cagcgtccag cctccgtggc cgcggtcgcg
      181 taccggcttg ccgatgtaca gcccctcggc gcggccgctg gcgaacgcct tgtagtactc
      241 ctcgccggca tgggacgttc ccaggtcgtc gccgtcgcga gacgaattca ccagcgtgcc
      301 gtcggcatcc agcaggtaca gcacggccag ttgccggatg ccgaagatgc gggcgctcat
      361 cagcaggcgg acggaaacac tgtcgaggtc cagttggctg ccataggtcg tctgcatccg
      421 ctcctgcacg ccccgcagca cgaggtcgac ccggtcgaac gtgtgctcgg tctcctcgcg
      481 gatcatgtgc gcgaagccga tggtgtccag ccgggccgtt tccagctcgc gcttgcgcat
      541 gtccaacaac aggaaaatga ccgatactgc gatcagcacg atcgtgagcg tcgccagcaa
      601 gccgatggct cgggtgggac ggatatgcat gcgcatgggc gtaaaggcgt cgtctgcggc
      661 cggacgtacc gcgaccagcc ctggtgtgta taggagttct ccgattccac accatggaca
      721 cggcgagcgg ttgacctacg tcaactgtcc tccaggctgt gccgacacac ttcaggcaca
      781 aaaagttgca aggcgtgcgg ccggtcgttc aagggcccct ccgcgagccc gatttgcccc
      841 taagtgcgcg ccagcacaca ctaattttga tagacggaaa actgagaatg cacatcgaga
      901 agagaagcct ttcacgcctc cggcgattcg cagtcgcctg ttggctggtc gccagcgcct
      961 gcctgggcgt gtccggcgcc ggtgcccaga ccgccgacgg cgccgcggcc gtgaaactgg
     1021 acaacgacac ctgcctcacc tgccacgacg cgaagaagcc gaaaattgaa agccgggacg
     1081 ccgccggcaa ggcacgcgcc ttgcacgcca tcccctcgga caagttcgag cagagcgtgc
     1141 actccatgat gcagtgcgtg agctgccact cggacatcaa ggacaacgcc cagaaaggca
     1201 atgcacacgc caaggacacg gccgaaccgt tgaaaaaggt ggactgcgcc aactgccacc
     1261 aggagctgtg ggagcagacg caaaagcgcg gcagggccgc cgacaggcca cggctgggcg
     1321 tggtcgcgaa gaacctcgag gcttacaaga agtcgtttca cgcgcgtccc aacgcggacg
     1381 acaagaccaa gccgaatgcg acatgcgaca actgccacga cgtgcacacc ttcaatgtgc
     1441 cgcccagaaa cacgccggaa catacgcagt ggcgcctggg cattccgggc atgtgcggca
     1501 cctgccatgc cgaccagctc gagtcttacg acaaatcggt gcacgcccag gtcaacagca
     1561 acaaactgct tgccgacgct gccgtgtgct ctgactgcca cacggcccat gcggtgaccg
     1621 gcacctcggg cagtcccttc aagctcacgg tgacggccaa ttgcggcaac tgccacaagc
     1681 aggagtacga ctcctacaag gacaccttcc acggcaagat caccacgctg ggttacagct
     1741 acaccgcgaa atgctatgac tgccacggca gtcacgagat tgccagaatc gcaagcacag
     1801 agtcgccggc caaccccgcg aaccggctca agacctgcca gtcgtgccac aaccctgaga
     1861 agggcctgcc agacgtgtcg gcgggctttg ccagtttcca gccgcacggt aacgtacacg
     1921 acgtctccaa gtacccgcag atgtggtttg ccttccagat gatggtgggc ctgctggtgg
     1981 gcacctttgc tttcttctgg ctgcacaccg ccttgtggtt ctaccgcgaa tacaaggacc
     2041 gccaggcacg cggcggcgga gaggcccatg tccggctcga cgaggtgccc gcccccttgc
     2101 gcggaaagca cgtccagcgc ttcaaccgga cctggcgcat cgcccacctg actttcgcgc
     2161 tgagcctgat ggtgttgaca ctgaccggaa tgccgctgtt ctattcggag gtgacctggg
     2221 cgcccgccat catgaacgcc ctgggcgggc cgcaggtcgc cggcatcatc caccggacgg
     2281 ccgccgtgat cttcgcgggt gtgttcttgt ggcacctggt ctacatgatg ttcaccctcg
     2341 gccggaaatg gaggaccttc aagctgttcg gcccgaattc gatggttccg agcctgcagg
     2401 acggcaagga catgctggcg atgttcaagt ggttcttcgg caagggaccg cgccccgtcc
     2461 tggaccgctg gacctactgg gagaagttcg actactgggc gcccttctgg ggggtcacca
     2521 tcatcggtgt cagcgggctg gtcatgtggc tgcccggctt cttcggtagc ttcctgccgg
     2581 gctgggtctt caatgtcgcg gccatcttcc acggtgaaga agcgttcctg gcggtggtgt
     2641 tcctgttcac ggtgcacttc ttcaacaacc acttccggcc cgacaagttc ccggtcgaag
     2701 tcgtgatgtt caccggcacg atgtcgctgg aagagttcaa gaaagaccat ggcgtggagt
     2761 accagcgcct ggttgacagc ggcgagctgg aacgccatct ggtcgaaccg ccgtcgacat
     2821 tcatggtgaa agggtccaag ctgctggcat tcgtcctgat catcgtcggc ctgaccctgc
     2881 tcacgctggt cggtgtcggg tttttcaccg gtggctgacg gcactcgctg agtgaggatg
     2941 cccgccgtct tccgtgggga cggcgacgcg cgaggcagta atgctgtttt aatagcaagg
     3001 ctgccgagtt gttgcagtag ttgttttttc ttcataggag acagaaaatg aagctgatta
     3061 ccaccttggc cgttgcctgc gggctgatga cggcatccat cgccaatgcc gccgtcgacg
     3121 ccgctgcggc gcaggcgtcc gtcaaagaga gcggctgcct ggcgtgccac gccatcgaca
     3181 agacgaaagc cgccacgtcc ttcaaggacg tcgcgaagaa atacaagggc aaggcggacg
     3241 cgcaagacaa gctggtcaag cacgtcaccc tcaagccgat ggtcgaaaag gacggagaga
     3301 aaatgccgca caaggcgctc aagaacaccg cccccgcggc ggtcaccaat gtggtgcagt
     3361 ggattctgtc gttgtagtga ttacacggcg ttgacaagaa ctggtcgggg ttccggtgcc
     3421 tcccggcagg cgccggcccg accgcatttt cattccccag cgtaagcaag ccagcgtgct
     3481 gaggcgtttt attggagtga cggcaaatgc ccgatagcat tctcgcaaag gttcgacggt
     3541 ggggcgcagc caggctggcg ctccttgtgt ggctcgccag cgccgccgtc atgccgggtt
     3601 tgagcgccgc tgcgccaccg gcgccggcgc agccggttgc cgctgctgac ttgcgggcgg
     3661 tgtttgcaac tccgcaggac atcgcggagg gcaagcgggt ggcgctggcc tcatgcacca
     3721 gttgccacgc ggtcactggg atagcactgg caaggggaac accccacatc gcgggccagc
     3781 gtccggcgta cctgtatgcc gaactgcgcg tgtaccaggc cggccgccgt ggcaacaacc
     3841 cgatgcacaa cgtggtcaaa ttcctgagcg acgatgcgct ggtcaaggta gccgcctact
     3901 acgcgagcct cgaccccgcg ccgcctgcgc ccgcagctgc caagaaggcg gcgagcaagg
     3961 cggatccggt cgccgccggc gaggccgctg cgtcgggctg ttccggctgt cacggtgaca
     4021 acgggatcag cgaaacaccc ggaatgccga acctcgttgg cctggatgcg aaatacatcg
     4081 ccgacgcgac cgtcgcctat aagaacggcc agcgcaaaca cggcatgatg aagacgctgg
     4141 tgtcggccct cagcgaagcc gaaatcagga acatcagcgt gttctatgcg ttgcagaaac
     4201 ccgccaaggc caagaccccg gcggaaggca acaaggaggc gggcaaagcc gctgccgccg
     4261 catgcgcggg ctgtcatggc cagggcggcg taagcaccgg caccgcgcca agcctcgccg
     4321 gccaggatgc gcagtacttc gccacggcaa tgaatgctta caagaccgga gcgagagccg
     4381 acgcagccat gaaggcgccg gcggcatcgc tggacgacaa ggtcatcaag gacctggcgg
     4441 cctactatgc cgaccaggcg ccgcagccac cccgcgtcaa aaaacccatg acgaccgagg
     4501 aaatcgccga tcgctgcaac cgctgtcacg gcgtcgacgg caacagcacc gatccgcgca
     4561 ctccggcgat cgcggcgcag cgcgtggact acctggaacc cgtcatgcgt gcctaccgca
     4621 agggcgagcg caagagcacg gcgatgaccg cgatgctgga cggaatgagc gacgaagaca
     4681 tcgcggaact tgccgtgcat tacgcaagca agaaggcgcg cagcgtggtc tacgtcccgg
     4741 tgcccaccaa gccataagga caaaacatgt cgacaccacc gatcgcgctg cacccgtctg
     4801 cccagcctga agagtccgag aagtccgaga agtccgagaa gtccctcatg agccagccac
     4861 aactgcaccg actctacaat cattattcag agttgccctt cagcatgcgc atcctctaca
     4921 cgtgcgtcct gtgcgttgtc ggaatgggct acctcttcgc gttcatttac ctgttccaca
     4981 cctattcggg gcgcgatggc caggccggcc tctcgtacga cgatctggtc atcgcctata
     5041 ccggcagcgg caagggttcg agactggagt cggcgctgcg cgggccgatg tcgacgatgc
     5101 tgcccgccga cgaagcgaca gcactgatat cgtgggcgca gcaaggtggc gagcgcgcaa
     5161 cctacgacac gttcgtcgcc cccttgctgg acaagcgctg cgtgagctgc cacgacggca
     5221 gcaaccctca cctggccaat ctgggcggct acgacaacgt gaagaaggtg acggaacagg
     5281 acaagggaac cgacatcttc acgctggttc gcgtctctca tatccacctg ttcggcatcg
     5341 tcatatttct ctacgtcgta ggcacgatct tcggccatgc atacgtgcgc ccgctctggg
     5401 tcaagtacgt ggccatggcc gcgccgtttg tcgctttgat gatcgacgtc agttcctggt
     5461 acctggtcaa gctctaccac ccgtttgcct ggctgacgat ggccggcggc gccatgctcg
     5521 gcctgtcctt tgcattcatg tgggtgatct cgatgtacca gatgtggatc gggaaaacgc
     5581 cgaaggcagt actcgagcgg ggcagcgaag cgatcggatg attgcgctgt cacacgccga
     5641 cgctttgccc ggcacaaggg caacgctgga tacagattac agacgaattc actttcgaga
     5701 tattccatga agaatgtatt ggcaggtatc gtgctgacgc tgctggcgct ggcggcatgt
     5761 acccagaagg acgaaccggc tccgaagccc gtcgccaccg acatcgcggc cggcaaggtc
     5821 attgcggagc gcgagtgcaa ggcttgccat ggggccaacg gcggaggcgt tgcgcccgcc
     5881 attccccacc ttgcggcgca gcgggagagc tatctgatgg cgtccttgaa cgaatacaag
     5941 gacggcaagc gctcgcatgc ggcactcaag gacttgacca aacagttgag tgaagccgat
     6001 ctgcgcaatg tggccgccta tttcgccagc ttgccgcccg ttgcccatcc caccgcccag
     6061 gatgtcaagc actcgtcgcc gtatgagaag ggcaaagcgc tggcggccgc ctgcgcgggc
     6121 tgccacggcg aagacggcaa cgccacgacg cccggcacac cgagcctggc cggccagcag
     6181 ccccattacc tcgttgcggc gatccaggag taccaccagg gagatcgcgc cggctccatg
     6241 aggtcgcaac tgcgcgatgc ggaccggatc gaactggaga atttggccct gtacttcgcg
     6301 gcacaaacac cggctcagcg tgctccggca tcgcgcggtg atcccgttgc gggcgagccg
     6361 ctcaccgcga tgtgcggcgg ctgccacggt gcgcgcggcg tgagcagcga cgcggcgaca
     6421 cccagcctcg ccggacagga cgcgcagtac ctggtggatg cgaccaaggc ataccgcaag
     6481 acacgccaga actggggtat gcagcgatat gtgtccggct tgagcgacaa ggacatcgac
     6541 aatattgccg cgtactatgt tgcgcagaag ccgcgggcgg cggaccaggt gccgagctcg
     6601 acccaggacc tcgcggccaa gtgcgatcgc tgccatgacg cggagggtaa tgcctcggcg
     6661 ccggcgccta aaatgcgcgg ccaggacaag gactatctga tcatggcgat gagggcgtac
     6721 cgcgacgaca agcgcgaaag ttcaaccatg cacagaatga gcggcgcgta cagcaatgcc
     6781 atcatcgaaa gcctggcgtc ctggtactcc agccagccgc ccaagtgagc gtcaagaaaa
     6841 ccgcgaaaag ccgcccgtgg gcggcttttg caattcccgg gccctaaccc tcgtggcggc
     6901 gagcaccgcg caccgcgagc atcgccgcct cgacgcggct gcgcacatgc agcttttcga
     6961 gaatgttggt catgtaatgc ttgatggtct tctcggatag atgcaggcga tcgccgattt
     7021 cccggttggt caacccgagt accaccagat tgagaatctc ggactcgcgt tcggttaatt
     7081 cgcccagcgg atcctgcggc gcctggccct gactgagcgt gaccagaatg cccgcggcca
     7141 gcgaaggggt gacatagact tccccttgag cgacggagcg cacgacgcgc gccagatcgt
     7201 gtgcggagac acccttgagc acatacgcgc gcgcaccggc cttgaacgat gccagcaact
     7261 tgtcctcgtc ctccatcacc gtgagcatca tgatccgtgt agcggggcag gcggtcctga
     7321 tcttttcggc cgtgacgatg ccgtcccagc cctgcatgcc gatatcgagc aggacgacat
     7381 cgggaagaag gtcgcgcgcc atgcgcagcg cctcctcgcc ggaggccgcc tggccgacca
     7441 cggcgatatc cggttccgat gcgagcgagt gcaccacacc ttcgcgaaac agcggatggt
     7501 catccgccac cagaatgcga atactctcag ccatactcgt gtgcctgctg ggccagaggc
     7561 agcgacgcgc ggacgacggt tccccgctcg gcggcactct cgacgtcgaa gcgtccgccg
     7621 agaagctcga cgcgttcgcg cattccttgc agccccagat gcccgccgtc gatcgatgcc
     7681 ttggggtcga accccttgcc ttcatcgctg acctcgacga tgagctgcgc atcgacaact
     7741 tccagtgaca cccgctggct gctcgtgccg ccgtggcgat aaccgttggc gagcgactcc
     7801 tgcaatagcc tgaacagcgc aatgcgcacc gacagcggcg cctcatcggg gacattgccg
     7861 atggaaagcg gtaccgccag gcccgtcttg tgttcgtggt ctctcaccgc gcggcgcgcc
     7921 gtatcggcca ccgaaagctg ctcgatttcc ggcaggcgca gcccgcgcag gatggcgcgc
     7981 atgtccgtca gcgcggactg gagcgcgacg tggatggtgt gaaactcgtc ggcgatgctg
     8041 cggtcgtgcc cgcttccctg cgcgcaggcg ctgcaaactt cggccagcga ttcgatgcgc
     8101 atcgacgcca gcgccaggcc ctgccccggc ccgtcgtgca ggtcggtggc gacgtggcgc
     8161 aggaagcgtt cgttgagcgc tgtcgtgcgg gcgccggcgc ggcggacccg gtcgtgcaac
     8221 tgctcgttct gggccaggag tgccgttagc tggtccacct tttcgccgag ctcgcgctgt
     8281 tgcgccgcga tcgtgttggt cgcccttcgc accagtgtcg cgagcaacgc cagcatggcc
     8341 agtgtcacgg cgccgtgcac cgaccagctg cgcagttgcg cctcgcgcgc cgcctgctcc
     8401 atgccgtccg ccgcctggta gaagtccgcc accgcgatga ccgagcggtc actatcggcg
     8461 cgaatgggtg agtaggtctg gatcaatcgg gaccgtttcc gttccttgaa gacgctctcc
     8521 aactcgctcg gctcgacgat gcgggagctc acctggccgg caaacgcgct tgccggcggc
     8581 cgcctggcca agtccggaac ggtcgtcgcc agcgacgggt cggaactgtc gaagatgacc
     8641 cggccttccg ggccccagat cttcagcacg acgatgcgct cgcccaggtg cgtgcttgcc
     8701 agctttcgct gaagtgcgat ccggtcggcc gcttcgagcg gccggccgtc cgccaacgac
     8761 tgcaagtgtg gagaaatggc gccgtccagg tacaggtcgg tcgtgacgcc catgccgttc
     8821 atgatgcccg actggatctg gctgcccacc caggtgccga tgaatatcat cgtcgcgacc
     8881 agcacaagga accccaccag aaggaaccgg tgcaccagac tcagcctgga tatcgctttc
     8941 atggcgaaca tcgaaaattc tagcacctgt ggttggcagc gggcagcgcc gaaggtccgg
     9001 gaccaaaggc ccgaacatcg tcccgattca ggttcaaagg tccgttgtcg cgcccctgct
     9061 gttgcctcat cctggcgaca aacagcaacc atggggacat catgaagtgt cggcaggcat
     9121 cgggagcgga ccgggctctc tcgtttttcc gcccgctttc cgggcgatgc cgcccgctgg
     9181 actcccggcg cttgcgcccg ggcaaggaga aaggcaatgc gaaggcgtcg tttcgcggtc
     9241 atgactctcc tggctaccgt gagcctggct ccgctttgcg gcgccgccca gctcgcgccc
     9301 gcatcaaaat cgaagcaaag cgtattccag gccacgctgg aaacccggcc ccaatcgacg
     9361 ccggagctgt cgacggacga tttcaaggcg ttcctggccg gcaagagcgg cgtcgtgctg
     9421 gatgcgaggc ccaagctgga gttcgagctc gcgcatattc ccggcagcat cagcatcgag
     9481 gcaacggggc tgctgcgcat cgtgcaagag ctgcccaggc cgacgaccgc catcgtcatt
     9541 tattcggacg gcccgttctc cgaacgcacg aagctgcgcg cggaagaact gctgagcatg
     9601 gggtacacga atgtcagccg ctaccagctt ggccttgcgg tgtggcgggc cctcggcaac
     9661 acagcggcaa ccagcctgga gggtttgcgt cgcatcttcc acgggaacat cgccgtgttc
     9721 gttgatgcgc gcagccgtgc cgagtatgcg gcgggcacga tccccgccgc ggaaacggta
     9781 ttggccggcg aggtgggcaa tgcgaggagg gaccaccggc ttcaatacca tgaccatgcc
     9841 acccgcatcg tcgtattcgg gaacagcgcc ctcgaagcgc gcacggtggc ggaagaaatc
     9901 gcccggaacg cgtactcgaa cagcagcttc ttcagtggaa cctatcaaga gctgaaacaa
     9961 gcgaagttct tttccgaacg aaagccatct cccctcaatc tggacggcgt tacgcgctga
    10021 cgtgaaagaa cgatcatgcc caagcacata ttccgactgc tctgcctgtt attcggtgtt
    10081 ctggtgctgg cgttcattgc ggcgaaagtc atgaccgccg attccttcta caagttcggc
    10141 cactaccgag ccgattcggt gaaggaaatt gccgcgcacg agcccgtcta ccaggcagcg
    10201 aattactgcc tgtcctgtca tgtcaagcgc gtcgcccaat ggtcggccaa cagccacaaa
    10261 tccgtcacct gcgaagtgtg tcacggcgcc gccctgggcc atcccgataa cggcaaactg
    10321 ccgatcccca aggacacggc gaagttgtgc accttgtgtc acgaggcaat gcccggacgc
    10381 ccccataccc agccccagat cgtgctggcg caacacaacc ccgtagccac gaccggacag
    10441 caatgcaccg cctgtcataa cccgcattcg cccaagatca cggcggggct ggtgaaggtg
    10501 acgggcaatg cggcggccgg caagcagcgt gccgcggact gcgcggattg ccacggcgag
    10561 ga
//
LOCUS       DASWIY010000457         3644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2542535, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000457 DASWIY010000000
VERSION     DASWIY010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542535"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..86)
                     /locus_tag="VGA59_16420"
     tRNA            complement(2..86)
                     /locus_tag="VGA59_16420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Leu,seq:cag)
     gene            142..2376
                     /gene="rnr"
                     /locus_tag="VGA59_16425"
     CDS             142..2376
                     /gene="rnr"
                     /locus_tag="VGA59_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX9721306.1"
                     /translation="MLDEIEGVIQGHRDGHGYVVRDDGELDIYLPPNEMRAVLHKDRV
                     KARIVRHDRKGRPEGRVVEIVERPPHPIIGRLLHESGVWLVAPEDKRYGQDVLIPKGA
                     TGLAKTGQVVVVELTEPPSLYGQPVGRVKEVLGEIDDAGMEIEIAVRKYAVPHEFSDA
                     CIGLARSLPDKVRPQDKKHRVDLTDIPLVTIDGEDARDFDDAVYCEPAKVGRGKGWRL
                     LVAIADVSNYVETGTAIDVDAYDRATSVYFPRRVIPMLPEKLSNGLCSLNPEVERLCM
                     VCDMLITGTGEIQAYQFYPGVMWSHSRFTYTEVAAILANTRGAEAVRHKDRVGDLLNL
                     YDVYRALLKARNQRGAVDFETTETQIICDENGRIERIVPRVRTDAHRLIEEAMLAANV
                     CSADFIQESKHPGLYRVHEGPTPEKKEILRGYLKAIGVGLTISSDPQPAEFQRIAEAT
                     KERPDAQQIHTMLLRSMQQAIYTPINSGHFGLAYDAYTHFTSPIRRYPDLLVHRVIKA
                     ILARERYELPVLPTPGEAHEKLARRLASRVSPPGHRLKKPAPPPSREVQAWEAAGLHC
                     SANERRADEASRDVEAWLKCKYMREHLGEEYGGIVTAATSFGIFVTLDQMYVEGLVHI
                     TELGGEYFKFDEARQELRGERTGIRYAIGTRLRVQVSRVDLDGRKIDFRLVREGEELT
                     SRALKDKGVTGNGRGAAAGVPAKSSTKRAARKASAEPQSPIQALKSAVKTAANKGKAR
                     KIRR"
     gene            2386..3150
                     /locus_tag="VGA59_16430"
     CDS             2386..3150
                     /locus_tag="VGA59_16430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9721307.1"
                     /translation="MATTTSGGKVAIVTGGGSGIGKAAALALLADGWRVAIAGRRREK
                     LDEAAAEAGAGGRALAVPTDVTDPASVEALFAATVKTWGRVDLLFNNAGVGAAAVPVD
                     ELPLEQWKTVVDTNLNGMFYAIREAFRVMKSQTPMGGRIINNGSISAYAPRPHSIAYT
                     ATKHAVSGLTKSASLDGRKYDIAVGQIDIGNAATELAARMAKGVPQANGEIAIEPLMD
                     VKIVGQSVLYMANLPLEANVLFHTVMATKMPFVGRG"
     gene            complement(3235..>3644)
                     /locus_tag="VGA59_16435"
     CDS             complement(3235..>3644)
                     /locus_tag="VGA59_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP/ATP-dependent (S)-NAD(P)H-hydrate
                     dehydratase"
                     /protein_id="HEX9721308.1"
                     /translation="PTVLTPHPLEAARLLGCDAAQVQRDRLLAGRELVQRFGCVVALK
                     GSGSVIAGPAAAPTINSTGNARLATAGTGDVLAGMIAARLAAGAGAFDAAAGAVYAHG
                     LAADEWPAGMPLTASALAQALSCNPYPRLSSRT"
BASE COUNT          704 a         1168 c         1249 g          523 t
ORIGIN      
        1 ttggtgccca ggaagggact cgaaccccca cgatgttact cgctagtacc tgaaactagt
       61 gcgtctacca attccgccac ctgggcattt caggaaagaa aaggatttta tatcaaaaat
      121 attggacaca tcagcacagc gctgctcgat gaaatagagg gagtgatcca gggccatcgc
      181 gacggccacg gctacgtggt tcgcgacgac ggcgagctgg acatctacct gccgcccaac
      241 gaaatgcgcg ccgtgctgca caaggatcgc gtgaaggccc gcatcgtgcg gcacgaccgc
      301 aagggccggc ccgaaggccg cgtggtggag atcgtcgagc gcccgccgca ccccatcatc
      361 gggcggctgc tgcacgaaag cggcgtgtgg ctggtcgcgc ccgaagacaa gcgctacggg
      421 caggacgtgc tgatccccaa gggcgcgacc gggctggcca agaccgggca ggtggtcgtg
      481 gtcgaactca ccgagcctcc cagcctttat gggcagcccg tggggcgcgt gaaggaggtg
      541 ctgggcgaga tcgacgacgc cggcatggaa atcgagatcg cggtgcgcaa gtacgccgtg
      601 ccgcacgagt tctcggacgc ctgcatcggg ctggcgcgtt cgctgccgga caaggtcagg
      661 ccccaggaca agaaacaccg ggtcgacctc accgacattc cactggtcac gatcgacggc
      721 gaggacgccc gcgacttcga cgatgcggtg tactgcgagc ccgccaaggt ggggcgcggc
      781 aagggctggc gcctcctggt ggccatcgcg gacgtgagca actacgtgga aaccggaacc
      841 gcgatcgatg tcgacgcgta cgaccgcgcc accagcgtgt atttcccgcg ccgcgtgatc
      901 ccgatgctgc cggagaagct gtccaacggc ctgtgctcgc tgaaccccga ggtcgagcgg
      961 ctgtgcatgg tctgcgacat gctgatcacc ggcaccggcg agatccaggc ttatcagttc
     1021 tacccgggtg tcatgtggag ccattcgcga ttcacctaca ccgaggtcgc ggcgatcctg
     1081 gccaacacgc gtggagcgga ggccgtgcgc cacaaggacc gcgtgggtga cctgctgaac
     1141 ctgtacgacg tctatcgcgc gctgctcaag gcgcgcaacc agcgcggcgc cgtggacttc
     1201 gaaacgaccg aaacccagat catctgcgac gagaacggcc gcatcgagcg gatcgtgccg
     1261 cgcgtgcgca ccgatgcgca ccggctgatc gaggaggcca tgctggccgc caacgtgtgc
     1321 agcgccgact tcatccagga aagcaagcat ccgggcctgt accgcgtgca cgagggtccg
     1381 acgccggaga agaaggagat cctgcgcggc tacttgaagg cgatcggcgt cggcttgacc
     1441 atcagctccg atccgcagcc ggcggagttc cagcgcatcg cggaggccac caaggagcgg
     1501 cccgatgcgc agcagatcca tacgatgctg ctgcgttcga tgcagcaggc gatctacacg
     1561 ccgatcaaca gcggccactt cggcctggcc tacgacgcct acacgcactt cacgagcccg
     1621 atccggcgct atcccgacct gctggtgcac cgggtgatca aggccatcct ggccagggag
     1681 cgctacgaac tgccggtgct gccgaccccg ggcgaggccc acgagaaact ggcccgccgc
     1741 ctggcaagcc gggtgagtcc gccgggccac agactgaaaa agccggcacc gccgccgagc
     1801 cgtgaagttc aggcatggga agcggcgggc ctgcattgca gcgccaacga gcgccgcgcc
     1861 gacgaagcca gccgggacgt ggaagcctgg ctcaagtgca agtacatgcg cgagcacctt
     1921 ggcgaggaat acggcggcat cgtcacggcg gcaaccagct tcgggatctt cgtcacgctg
     1981 gaccagatgt acgtcgaagg gctggtgcac atcaccgagc tgggcggcga atacttcaag
     2041 ttcgacgagg cgcggcagga attgcgtggg gagcgcaccg gcatccgcta cgcgatcggc
     2101 acacggctgc gggtgcaggt cagccgggtc gacctggacg gacgcaagat cgatttcagg
     2161 ctggtgcgcg agggcgagga actgacgtcg cgcgcgctca aggacaaggg cgtgaccggc
     2221 aacggacgcg gggccgcggc cggtgtgccc gccaagtcct ccaccaagcg ggccgccagg
     2281 aaggcgtcgg ctgagcccca gtcgccgatc caggcactca agagcgccgt caaaacggcc
     2341 gcaaacaagg gcaaggcccg caagatccgt cgataggaga tctcactggc aacgacaaca
     2401 tcgggcggca aggtcgccat cgtcaccggc ggtggaagcg gcatcggcaa ggccgcggcc
     2461 ctggcgctgc tggcggacgg ctggcgcgtc gcgatcgcgg ggcgcaggcg ggaaaaactg
     2521 gatgaggcag cggcggaggc gggcgccggg ggccgtgcgc tcgcggttcc aacagacgtg
     2581 accgatccgg cttcggtcga ggcgctgttc gccgccacgg tcaagacctg gggccgtgta
     2641 gacctgctgt tcaacaatgc gggcgttggc gccgccgcgg tgccggtgga cgagttgccg
     2701 ctggagcaat ggaaaaccgt ggtggacacc aacctgaacg gcatgttcta tgcgatccgg
     2761 gaggcgtttc gcgtgatgaa gtcgcagacg ccgatgggcg ggcgaatcat caacaacggt
     2821 tcgatctcgg cgtacgcgcc gcggccccat tcgatcgcct ataccgcgac caagcacgcg
     2881 gtatcgggcc tgaccaagag cgcttcactg gatggccgca agtacgacat cgccgtgggt
     2941 cagatcgaca tcggcaacgc cgcgaccgaa ttggccgcgc ggatggccaa aggcgtgccc
     3001 caggccaacg gagagatcgc gatcgagccg ctgatggacg tgaagatcgt gggccagtcc
     3061 gtgctgtaca tggccaacct gccgctggaa gcgaacgtgc tgttccacac ggtgatggcg
     3121 acgaagatgc cgttcgtggg caggggctga attctccgcc tcgtcattgt gggcttgagc
     3181 ctgtcattgc gggcctgacc cgcaatccat gaccctgaat tcatggatcc cggatcaagt
     3241 tcgggatgac agccttggat aaggattgca tgacagcgcc tgagccaaag cgctggctgt
     3301 gagtggcatc ccggcgggcc attcatccgc cgccagcccg tgcgcataga ccgcgccggc
     3361 cgcggcgtcg aacgcgccgg cgccggccgc aaggcgcgcc gcaatcatgc cggccaacac
     3421 gtctcccgtg cccgcggttg ccagacgcgc attgccggtt gaattaatcg tgggggccgc
     3481 cgccggcccg gcgatcacgc tgcccgagcc tttgagagcg acgacgcagc cgaagcgctg
     3541 gaccagctca cggccggcca gcaaacggtc ccgctgcact tgcgccgcat cgcagcccag
     3601 caggcgcgcg gcctccagcg gatggggcgt caggacggtg gggc
//
LOCUS       DASWIY010000458        18739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3617895, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000458 DASWIY010000000
VERSION     DASWIY010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 18739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18739
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3617895"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..348)
                     /gene="rplU"
                     /locus_tag="VGA59_16440"
     CDS             complement(37..348)
                     /gene="rplU"
                     /locus_tag="VGA59_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX9721309.1"
                     /translation="MYAVIKTGGKQYRVASGEKIKVEQIAADVGQEIVIDQVLAVGNG
                     SEIKVGTPLVSGATVKATVVAHGKHDKVRIFKMRRRKHYQKRQGHRQQFTELEIGAIA
                     G"
     gene            534..1514
                     /locus_tag="VGA59_16445"
     CDS             534..1514
                     /locus_tag="VGA59_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9721310.1"
                     /translation="MTSSSTSTSVLSLIADDMLEVDAVIARRLESGVPLVAEVSRYII
                     SAGGKRLRPALLLLVSRALGHEGPQRFNLAAVVEFIHTATLLHDDVVDESTLRRGRPT
                     ANENFGNPASVLVGDFLYSRAFQMMLDAGQMRIMEILADATNVIAEGEVLQLMNMHDA
                     TLDEAAYLRVIRSKTAKLFEASARLGAVLAQAAPAVEQACADYGQALGTAFQVIDDVL
                     DYDGKAGEMGKNLGDDLREGKATLPLIAAMQRGTPAQRDLIRNAIETGGVAQLDRIVA
                     IVRETGALDVTRAAAATEAGRAVEAARLLPDNSYSQGLLQLAAQLLQRRN"
     gene            1545..1621
                     /locus_tag="VGA59_16450"
     tRNA            1545..1621
                     /locus_tag="VGA59_16450"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1579..1581,aa:Pro,seq:cgg)
     gene            1718..3577
                     /locus_tag="VGA59_16455"
     CDS             1718..3577
                     /locus_tag="VGA59_16455"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX9721311.1"
                     /translation="MPEADSWDPLPARAGTVDADRYGIQLYPQWRVLKGLAYGLKQAT
                     MLGKVHVAVPKGFSFTDLDEIVAPLFDELVPEPERPLDPASGLIHRLLSWQAAVQRQQ
                     KLPVLGSYMATRLATSKEPGHEYLVGVSYHAPRATMEACSRIVKIMNRVLHDPVSSPE
                     SHSRTRIEFEQLLQSLKTYALSGINNFHLLDAARGLDIPVRGIFPDTYCLGIGAHRRW
                     LQSTMTDRTPALGVNIAHSKSNTALMLREHGVPVPSHGAAGSEEQAVLAAHKLGFPVV
                     VKPDDQEQGRGVTAGLRDEEAVRAAYRRAREHSKKILVEKHHDGQDYRLTVYQGQVIK
                     ILNRRAGGVFGDGVHTIGELVDAEHQTTRFRKVYQQTGKLLLKLDEEALALLAQEGLA
                     PDSVPGAGEFVRLRSKSNISAGGIQTLIPVHDAHPDNLDLAVRATAAMHLDICGVDLL
                     IADISASWMETGAVIIEMNAKPQIGYSLAPEVYQLVVRGLVQGDGRIPVQLVICATQT
                     ECPGEDEALALMKASQCDGLSMSQGVWVDGRRLVARQQDGFAAARVLLLSDAVHSAVC
                     VMTADDILQNGLPADRFARITVRNKASASPREKSILHSALAMLGAGGPRTRFP"
     gene            3574..4521
                     /locus_tag="VGA59_16460"
     CDS             3574..4521
                     /locus_tag="VGA59_16460"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9721312.1"
                     /translation="MTPKQTLPRDLDGKAPQRARVLALGLDAPNHELLLQWMAGGQLP
                     NLQKLQKRSAAVTIGSTKRYSNEHCWIPMLTGQNTNRWNHWLDQWDATRYKFSEASIF
                     DWLQAPLFYARGSGLRVTSFDLTAPVVKNVEGIQIAGWASELNESYPQSEPAELLQEL
                     LTLYGPDPKLVGAKAVTNPLSDRQGVSHVVPNLYQPELMRDFALRQVRSVERRTAACR
                     DLLKRDKWDLFITMYSEIHTAGHVLWHLSQPHPLNMTREGPEDPLLTVYQAVDRSVGQ
                     LLEEIDPTVTVVFYTIDAIVTDSLENARAIFLPEFLYRW"
     gene            4478..5230
                     /locus_tag="VGA59_16465"
     CDS             4478..5230
                     /locus_tag="VGA59_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721313.1"
                     /translation="MRERFFCLNSFTDGNFPGKAALAEGIRGVAPSPARVDYAEHWKH
                     EVWRLRSTFGDTELESPASQEAREDPMNWCPANWYAPLWSSMRAFAMPTVADGYIRLN
                     VQGREAHGVVSAADYNTICDQLEKDLSGLVNARTGKPVVREIVRVRKSPIEDDPKQPP
                     ADLIVVFEEQGPVDTVESPLVGRIGPVPYFRSGAHQAQGGCVTNLMYVSGPGIQPGQR
                     SNQPAALEDIPATILSLLRVALPAEFDGMARI"
     gene            5246..6709
                     /locus_tag="VGA59_16470"
     CDS             5246..6709
                     /locus_tag="VGA59_16470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9721314.1"
                     /translation="MPTRLTLHLFESDPSLPQRDGVNLAHTEINCLLSNEADPDLQVH
                     FHDFARLLRDPAYAIEVLADVDCVLCNVGPHAHYYHYLRDQLGLNFRIVRDIKTALWS
                     GYLLQESLCDPYLRPGDTLLATSHYSRVLTQHIFPHLRSHSVHLFEPVLAYPAKHASA
                     KRFRVADGEKITLGYVGRLSEDKNFPQIVELLMALDQEEPGKYQLVAVGAVHSASCEP
                     SLIADKIYAKTGRNDLFRYLPPVAHDEVLTLLPQFDYFLFFSTSNLEVLGRVLLEAAH
                     TGVPILAANHAAAPELLAPTSLIDVHYTLEQTFHSHFDVPLGRIDIAMAASKIRRGEI
                     PNAPPEPQINRRETLMDILVQGPGEGKEYGLNSLPQAQRRFLERLRWEGLTRYSSRRE
                     AGSAIESMLDWFCALNGKSAHDFEFRLQELERRSRFQERTRRFMAAAARTRCDFTNLG
                     GIDVELCNIVGYHPQFRLSDLPQAQVNAAELANRREM"
     gene            6869..8608
                     /gene="pilB"
                     /locus_tag="VGA59_16475"
     CDS             6869..8608
                     /gene="pilB"
                     /locus_tag="VGA59_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEX9721315.1"
                     /translation="MAAVDPVAAEAPPTALPGLARALISAGKLGQKSAEEISRKAQAN
                     RNSFIAELTGSGAVSASDLAHTMSTAFGAPLLDLAAIDLQRLPKNLLDNKICQDFRVV
                     VLSKRNNRLIVATADPSDQQAGEKIKFATQMGVDWIIAEYDKLTKMVEAGTASANETM
                     DNIIGEEFEFDESSIEATTSGDDNDAATSEVEDAPVVKFLHKMLLDAFSMRASDLHFE
                     PYETNYRVRFRIDGELREIASPPVAIKEKLASRIKVISRMDISEKRVPQDGKMKLKIG
                     PDRVIDFRVSTLPTLFGEKIVIRILDPSSARVGIEALGYEPDEKARLMAAVGRPYGMV
                     LVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPSEINMPGINQVNVNEKAGLTFAAA
                     LKSFLRQDPDVIMVGEIRDLETADIAIKAAQTGHLVMSTLHTNDAPTTLTRMRNMGIA
                     PFNIASSVILITAQRLARRLCPNCKAPADIPHETLLEAGFADEDVDGTWTPYRPVGCS
                     ACNNGYKGRVGIYQVMPISEDIQRIILADGSALEIAAQAAREGVRNLRQSGLHKVKLG
                     VTSLEEVLGCTNV"
     gene            8630..9838
                     /locus_tag="VGA59_16480"
     CDS             8630..9838
                     /locus_tag="VGA59_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9721316.1"
                     /translation="MAMAATKEFVFEWEGRDRNGKQVRGETRAAGENQVQAALRRQGV
                     SPTKVRKRRMRSGAKIKPKDIAIFTRQLATMMKAGVPLLQSFDIVGRGNPNASVTKLL
                     NDVRTDVETGTSLSAAFRKYPLYFNNLYCNLVEAGEAAGILEALLDRLAVYMEKTEGI
                     KSKIKSALMYPISVVVVAFVVVAVIMIFVIPSFKEVFASFGADLPAPTLMVIALSEFF
                     VAYWWLIFGGIGGGFYFFMQAWKRSEKVQMFMDRVLLKVPVFGDLVYKSVIARWTRTL
                     STMFAAGVPLVEALDSVGGASGNSVYALATEKIQMEVSTGTSLTAAMTNAGVFPSMVL
                     QMCAIGEESGSLDHMLGKAADFYEAEVDDMVAGLSSLMEPIIIVFLGTLIGGIVISMY
                     LPIFKLGQVV"
     gene            9838..10680
                     /locus_tag="VGA59_16485"
     CDS             9838..10680
                     /locus_tag="VGA59_16485"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX9721317.1"
                     /translation="MIFSPVIDAALAGVLGLLVGSFLNVVIYRLPQMLERQWAAECAE
                     LAGKPAQEAPAFNLVSPRSRCRQCGHLIRWYENVPILSYLVLRGKCSACGTAIGLRYP
                     LVELATAILFFFSVRHWGATPAGAAWCGFSAAIVALALIDWDTTLLPDDITLPLLWAG
                     LIVAALRWTDVTLPQALWGAVAGYVSLWLVYQGFKLLTGKEGMGYGDFKLFAALGAWF
                     GWTALVPIILMASVIGAVVGIAMKLSSGLREGGYVPFGPFLAGAGLTAMVFGPASILG
                     VIGL"
     gene            10729..11334
                     /gene="coaE"
                     /locus_tag="VGA59_16490"
     CDS             10729..11334
                     /gene="coaE"
                     /locus_tag="VGA59_16490"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800183.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX9721318.1"
                     /translation="MAAIRVGLTGGIGSGKSTVLQMLERLGAAAIDADAISRRTTAAG
                     GAAIPAIAARFGNEFLTPEGALDRDRMREHAYADPGARILLEEIIHPLVGAESTRQVQ
                     AAVAAGVRCIVFDIPLLVESGRWRAQVDRVLVVDCSPRTQVDRVVARSGRTPEEVLAI
                     VAVQATRGQRLAAADLVICNEGMSLEQLRDKVVQTARRFGL"
     gene            11377..12132
                     /gene="zapD"
                     /locus_tag="VGA59_16495"
     CDS             11377..12132
                     /gene="zapD"
                     /locus_tag="VGA59_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEX9721319.1"
                     /translation="MILYEYPFNERIRTYLRLEHLFRRLGELVPRSHPIDHHYALTTI
                     FEVMDVAARADLKSDVMKDLEKQKQLLNGYRGNPAIAEGVLDEVVAKLERCFATLNNL
                     PGKAGQSLTENEWLMSIRSRVGIPGGTCEFDLPAYYAWQHRTADKRRTDLERWANTLA
                     PLAESIHLLLKLLRDSGTPQKVMASHGQLQQTLPQGRTFQLLRLRLDPALGLVPEISG
                     NRLMVSVRLMRQESADRLHASNDSAQFELTLCS"
     gene            12145..12339
                     /locus_tag="VGA59_16500"
     CDS             12145..12339
                     /locus_tag="VGA59_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX9721320.1"
                     /translation="MARPHPPSDANPRLVRCPACGGESVYAPANLYRPFCSERCKNMD
                     LGAWASEEFRVPEKPAPDDE"
     gene            complement(12415..12888)
                     /locus_tag="VGA59_16505"
     CDS             complement(12415..12888)
                     /locus_tag="VGA59_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9721321.1"
                     /translation="MKPGPLVADGDRPREGGPDRKEIEVAVGVLIRADGDFLLTSRPT
                     GKVYEGYWEFPGGKVEPGESVEQALRRELLEEIGLRIGPVHPWRVERVDYPHALVRLN
                     FCKVFEWTGRLHMHEGQSFAWQNLPVQVRPVLPGTVPVLQWFAQERGYAGETHDK"
     gene            complement(12885..13757)
                     /locus_tag="VGA59_16510"
     CDS             complement(12885..13757)
                     /locus_tag="VGA59_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9721322.1"
                     /translation="MNEKFEQLIERAGHLLSRIESILPQPLSSPDWRASMAWRYRKRS
                     SGHGALEPVRHRAAIRLADLKEIDTQKEKIQRNTLQFVQGQPANNVLLTGARGTGKSS
                     LIKACLNEYAGRGLRLIEVDKADMVDLPDIVDVVASQPEKFIVFCDDLSFEEGEPGFK
                     ALKSILDGSVAAATPNVLIYATSNRRHLLPEYMKENLSYTHTPDGEVHPGEVVEEKIS
                     LSERFGLWVSFYPFSQDEYLAITAQWLSSFGVDGTAIEAARPEALVWALERGSRSGRV
                     AYQFARDYAGRHPA"
     gene            complement(13812..15038)
                     /gene="argJ"
                     /locus_tag="VGA59_16515"
     CDS             complement(13812..15038)
                     /gene="argJ"
                     /locus_tag="VGA59_16515"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX9721323.1"
                     /translation="MAVNLPAPRAADLHPVPGVRVGVAEAGIRKADRKDLTVVLIDPG
                     ASVAGVFTQNRFCAAPVQVCRDHLARGDVRALVINTGNANAGTGEDGLVRARTSCIAL
                     ARRLEIAPEQVLPFSTGVIMEPLPVERIEAGLPAALADAGPDNWLRAAEAIMTTDTVP
                     KAFSRRVVIEGAPVTITGISKGAGMIRPNMATMLGFLACDARLDQAVMRQLALELAEG
                     SFNRVTVDGDTSTNDSFVVIATNKAAHATITSLDSASGQALKAAMLDVARQLAQAIVR
                     DGEGATKFITLRIEGGKTAQECRKVAYAIAHSPLVKTAFFASDPNLGRILAAVGYAGI
                     DDLDQTRIDLHLDDVHVAVHGGRNPAYREEDGQRVMKQGEITVRVGLGRGSAADTVWT
                     CDLSHDYVSINADYRS"
     gene            complement(15082..15450)
                     /locus_tag="VGA59_16520"
     CDS             complement(15082..15450)
                     /locus_tag="VGA59_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9721324.1"
                     /translation="MPQLAFDWWQKGEAVLVDVRTDAEREWVGFVPGAVPLAWKQWPG
                     MAINSAFDDGIRAAVPPGRKAVLFCRSGVRSISAARRATELGIEAYNILEGFEGEPDD
                     KAQRGKRGGWRFHGLPWRQG"
     gene            complement(15715..16218)
                     /locus_tag="VGA59_16525"
                     /pseudo
     CDS             complement(15715..16218)
                     /locus_tag="VGA59_16525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(16290..16451)
                     /locus_tag="VGA59_16530"
     CDS             complement(16290..16451)
                     /locus_tag="VGA59_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721325.1"
                     /translation="MKLVFGLLGAILMVAFLTSFVIKVKEISMVVVALIGIALMLVDL
                     WHARNEPDT"
     gene            complement(16465..17814)
                     /locus_tag="VGA59_16535"
     CDS             complement(16465..17814)
                     /locus_tag="VGA59_16535"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9721326.1"
                     /translation="MYLLSATEMARRLREGECSAEDYARALLARVDEVEPQVQAWEFL
                     DREYLTEQAADADLRHQRGAETGPLSGIPVGIKDIIDVRGMPTGDGTPLHAGRMPRSD
                     AALVRQLRRAGGIPMGKTVTTELATYAPGKTRNPHRDLAGREHTPGGSSSGSAAAVAA
                     GMVAMAVGTQTNGSVLRPASFCGVYGFKPSFGLIPRTGVLTQSPPLDQVGVFARSLED
                     IALLAQALCGHDEDDPATRAQPAPPMCKLVASQPPLPPRFAVVRTPFWERMDADAREA
                     FEDLAEELGDAGMPVELHDSAAEAVTWHRTIMEADLAASFAREFKSGRERLSPSLCAQ
                     IERGLQVSAVDYRHAVERIPSLVAYLDELFAEFDAIVTPATLGTAPGGLEGTGDPLMC
                     TLWTLTGLPAISLPLLSGVNGLPLGVQLVGGKGDDARLLRTARWLVRRLDRPQSDKA"
     gene            complement(17849..>18739)
                     /locus_tag="VGA59_16540"
     CDS             complement(17849..>18739)
                     /locus_tag="VGA59_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9721327.1"
                     /translation="ICAGGTLGILIPPSIMLIVYGATAGVSVVRLYAAALLPGFFLAG
                     LYMIYVVFRALLNPGLMPKLAEDQHRMDFLAVVALMLKAFFPLAFLIMSVLGVILFGL
                     ATPSEAAAIGALGSVVLAVGYQAFTWQRLREAVFLTARTSAMVCYLFIGSWLFSSIFS
                     YLGGEALIKEFVLGMGLNTVTFLLLSQVIIFLLGWPLEWSEIIIIFVPIFLPLLPHFN
                     VDPIFFGILVALNLQTSFLTPPMAMSAYYLKGVAPAHVLLSSIFRGCMPFLGMVFVAM
                     IAMYVVPGFVTWLPGLMYGR"
BASE COUNT         3459 a         6046 c         5888 g         3346 t
ORIGIN      
        1 gagctgaaga tctgtcccgc aaggtcgctg gattggttag ccggcgatcg cgccgatttc
       61 cagttcggtg aactgctggc gatgcccttg gcgcttctgg tagtgtttgc gacggcgcat
      121 cttgaagatg cgaaccttgt cgtgcttgcc gtgagccacc acagtggctt tcacagttgc
      181 gccggacacc aggggagtgc caaccttgat ctcgctgccg tttccgacag ccagaacctg
      241 gtcgatcacg atttcctggc ctacgtccgc agcaatctgt tctactttaa ttttttcgcc
      301 ggaagcaacg cgatactgct tgcccccggt ttttatgacc gcgtacatgt tgacccttga
      361 aatcgaggcg ctcgcttctg agctggcgcc ccctacaaag aatgactccg cggacggatt
      421 tccgcagagc ccaaaactat agcatagttt gcggttactc acaacgtcca acggcccggt
      481 cggccggctc cagcgcccgc tttctataat cggtcaactc tcccgcccac cccttgacct
      541 cttcctccac cagcacttcg gtgctttccc tgatcgccga cgacatgctc gaggtcgacg
      601 cggtgatcgc ccgccggctg gaatcgggcg tgccgctggt cgccgaagtc tctcgttaca
      661 ttatttccgc aggcggcaag cgattgcgtc ccgccctgct gctgctcgtt tcgcgcgcgc
      721 tcggccacga agggccgcag cgattcaacc tggcggccgt cgtcgagttc atccacaccg
      781 cgacgctttt gcacgacgat gttgtcgacg aatccacgct caggcgcggc aggcccaccg
      841 ccaacgagaa cttcggcaac ccggcctcgg tgctggtggg cgacttcctt tattcgcgcg
      901 ccttccagat gatgctggac gcgggccaga tgcgcatcat ggagatcctg gccgacgcga
      961 ccaacgtgat cgccgagggc gaggtgctgc agctgatgaa catgcacgac gccacgctgg
     1021 atgaagccgc ctacctgcgc gtgatccgct cgaaaacggc caagctcttc gaggccagtg
     1081 cccgcctggg cgccgtgctt gcgcaggccg cgcccgcggt ggaacaagcc tgcgccgact
     1141 atggccaggc gctcggcacc gcattccagg tgatcgacga tgtactcgat tacgacggca
     1201 aggccggcga gatgggcaag aacctgggcg acgacctgcg cgaaggcaag gccactttgc
     1261 ccctgatcgc ggcgatgcag cgcggcacgc cggcgcagcg agacctcatt cgaaacgcca
     1321 tcgagaccgg cggggttgcg cagctggatc gaatcgtcgc gatcgtgcgg gaaaccgggg
     1381 cgctggacgt cacccgcgcc gccgcggcga cggaagcggg ccgggccgtg gaggcggcgc
     1441 gccttcttcc ggacaattcc tacagccagg gtttgctaca attggcggct caactgctgc
     1501 agcgccgcaa ctgatcgctc agtctcgcgg cggtatccag caaacggggt gtagcttagc
     1561 ctggtagagc gctacgttcg ggacgtagag gccggaggtt cgaatcctct caccccgacc
     1621 agatttcctt tcgacattgg ccccgtgttg cttgagcccc gtccgagccg cccgccgcgc
     1681 caacctgaat caagcacacc aggaggtgag caaccatatg ccggaagctg actcatggga
     1741 tccactcccc gcaagagccg ggacagttga cgcggatcgc tacggcattc agctttatcc
     1801 gcagtggcgg gtcttgaaag gcctggcgta tggcctgaag caggcaacca tgctcgggaa
     1861 ggttcatgta gccgtcccca aaggtttcag tttcaccgac cttgacgaaa tcgtggcccc
     1921 gcttttcgat gagctggtgc ccgaaccaga gcggccgctc gacccggcaa gcggtctcat
     1981 tcatcgactg ctgtcttggc aggcagcagt gcagcggcaa caaaaactgc cggttcttgg
     2041 ctcatacatg gccacccgcc tggcaacatc gaaagaacct ggccacgagt acctcgtggg
     2101 ggtttcctat catgcacccc gggccaccat ggaagcgtgc agccggatcg tcaagatcat
     2161 gaaccgtgtt ctgcacgacc cggtatcgtc cccggagtct cattcccgca cccggatcga
     2221 gttcgaacaa ctcctgcaga gcctcaagac gtacgccctg agcggcatca acaatttcca
     2281 tcttctcgat gccgcgcgcg gcctggacat tccggtcaga ggtattttcc ccgacaccta
     2341 ttgtcttggt atcggcgcgc accggcgctg gctgcaaagc acgatgaccg accggacgcc
     2401 cgccctgggc gtcaacattg cccatagcaa aagcaacacg gcgctgatgc tgcgggagca
     2461 cggcgtaccc gtgccgtcgc atggcgccgc gggcagcgaa gagcaagcgg tgctcgcggc
     2521 ccacaagctt ggctttccag tggtggtcaa gcccgacgac caggagcaag gccggggcgt
     2581 cacagccggc ttgcgggacg aagaagccgt gcgagccgct taccgcagag cccgcgagca
     2641 ttcaaaaaaa attctggtcg agaagcacca cgacggccag gattaccgcc tcaccgttta
     2701 ccagggccag gtgatcaaga tcctgaatcg ccgagccggt ggcgttttcg gggacggggt
     2761 gcacaccatc ggcgaactcg tcgacgcgga acaccaaacc actcggtttc gcaaggtgta
     2821 ccagcagacg ggcaagctgt tgctgaagct tgacgaggag gcgctcgcat tgcttgctca
     2881 ggaagggctc gcgcccgaca gcgttcccgg cgccggcgaa ttcgttcgtt tgcgcagcaa
     2941 aagcaacatt tccgctggcg gtattcagac gctgattccc gtccatgacg ctcatccgga
     3001 caacctcgac cttgccgtgc gcgccacggc ggcaatgcac cttgacatat gtggcgtcga
     3061 cttgctcatc gcagacatat cagcttcgtg gatggagacc ggcgccgtga tcatagaaat
     3121 gaatgcgaag ccccagatcg gctactcgct tgcgcccgag gtttatcaat tggtcgtgag
     3181 ggggctggtg cagggagacg gtcgtattcc ggtccaactg gtgatttgtg cgacacagac
     3241 ggaatgtccg ggggaggacg aggcgctggc gttgatgaag gcaagtcagt gcgacggact
     3301 gtccatgtcg caaggtgtct gggttgacgg gagacgcttg gtggccaggc agcaagatgg
     3361 gtttgccgct gctcgagtcc tgctgctcag cgatgctgtc cacagcgccg tctgcgtgat
     3421 gactgccgat gacatcctgc agaatggact gcccgccgac cgtttcgcac ggatcaccgt
     3481 acgcaacaag gcatcggcat caccccggga aaagtcgata ctgcatagcg ctctcgccat
     3541 gctgggtgcg ggcggacccc gtactcgttt cccatgaccc ccaagcagac tcttccccgc
     3601 gacctggacg gaaaggcacc gcagcgcgcc agggtgctgg cgttgggact cgatgcgccc
     3661 aaccacgagc tgcttttgca gtggatggcc ggtggacagt tgcccaacct gcaaaagctg
     3721 caaaagcgaa gcgcggcggt gaccatcggt tccacaaaac gctacagcaa tgagcattgc
     3781 tggataccaa tgctgactgg gcaaaacaca aaccggtgga accactggct cgatcaatgg
     3841 gatgccaccc gctacaagtt cagcgaagct tctattttcg attggctcca ggctcccctg
     3901 ttctatgcgc ggggcagtgg gctgcgggtg acatccttcg acctgacggc gccggtggtc
     3961 aagaacgtcg aaggtatcca gatagccggc tgggcgagcg aactgaacga gagctatcct
     4021 caatcggaac cggctgaact tctgcaggaa ctgctcacac tgtatgggcc ggatcccaaa
     4081 ttggtgggcg cgaaggccgt caccaatccg ctgtcggaca ggcagggcgt atcccacgtt
     4141 gttccgaacc tctaccagcc tgagctgatg agagatttcg ccctgcggca ggtgcgctcc
     4201 gtggagcgcc gcacggccgc gtgccgcgac ctgctgaaga gggacaagtg ggatctgttc
     4261 atcaccatgt attcggagat tcacacggcc ggccatgtct tgtggcactt gagccagccc
     4321 catccgctga atatgacgcg ggagggaccg gaggacccac tgctcacggt gtaccaggcg
     4381 gtcgatcgct ccgtcgggca gctgcttgag gagatcgacc cgacggtgac cgtcgtcttt
     4441 tacacgatag acgcgattgt cacggacagc ctggagaatg cgcgagcgat ttttctgcct
     4501 gaattccttt accgatggta attttccggg aaaggcggca ttggccgaag gcatccgggg
     4561 cgttgcgccc tcgccagccc gcgtcgacta tgcggaacac tggaagcatg aagtgtggcg
     4621 tctgcgcagc acgttcggcg acaccgagct tgagtcaccc gcgtcgcagg aagcacgcga
     4681 agaccccatg aactggtgcc cggccaattg gtatgccccc ttgtggtcat ctatgcgcgc
     4741 cttcgctatg cccaccgtgg cggacggcta tatcaggctc aatgtccagg ggcgggaggc
     4801 gcacggcgtg gtgagcgcag cggactacaa cacaatttgc gaccagctcg agaaagacct
     4861 gtctggcctg gtgaatgccc gcaccggaaa gcccgtggtt cgggaaatcg tgagggtccg
     4921 gaagtcgccg atcgaggatg acccgaaaca accgcctgcc gacctgattg tcgtgttcga
     4981 agagcagggg ccggtcgaca ctgtggaatc tccattggta gggcgcatcg gccccgtacc
     5041 ctatttccga agtggtgcac atcaagctca gggcggttgc gtgaccaact tgatgtacgt
     5101 gagcggaccg ggcattcagc ctgggcaacg gtccaaccag ccggcagcgc tcgaggatat
     5161 tccggcaaca atcctgtctt tgctgagagt ggccttacct gccgaattcg acggtatggc
     5221 caggatctga cggataacga ctgctatgcc aacgcgactt acgctgcatc ttttcgagag
     5281 cgaccccagc ctgccgcaac gagatggtgt caatctcgcg cataccgaga tcaactgcct
     5341 tctgagcaat gaagcagatc cggacttgca agtccacttt catgatttcg cgcggttgct
     5401 gcgggatccc gcgtatgcca tcgaagttct ggccgacgtg gattgcgtac tgtgcaacgt
     5461 cgggccgcac gcgcattact accactacct tcgggaccag ctggggctga acttccggat
     5521 tgtgcgggac atcaagacgg cgctttggag cggctacctg ttgcaggaaa gcctgtgcga
     5581 tccctatttg cggcccggcg atacgctgtt ggctacctcc cactattcga gagtcctgac
     5641 tcaacacatt ttcccgcacc tgcgcagcca ttctgtccat ctgttcgaac ccgtcctggc
     5701 atacccggcc aagcacgcaa gcgcaaagcg gtttcgggtc gcggacgggg agaaaataac
     5761 gcttgggtac gtaggacggc tctcggagga caagaacttc cctcagattg tcgaactgtt
     5821 gatggccctc gaccaagagg aacccggcaa gtaccagctg gtggccgtcg gcgcagtcca
     5881 ctccgccagt tgcgagccca gcctgattgc ggacaagatc tacgcaaaga ccgggaggaa
     5941 tgacctattc agatacttgc caccggtggc ccacgacgaa gtcctgacgc tgctgcccca
     6001 gttcgactac ttcctgtttt tttcgacgtc taacctcgag gtgttagggc gtgtcctgct
     6061 tgaagccgcc cacaccggcg ttccgatatt ggccgccaac catgcggccg cgccggaact
     6121 gctggcaccg acctctttga tcgatgttca ctacaccctg gagcagacgt ttcattcgca
     6181 tttcgacgtc cccttgggcc gaatcgacat cgcgatggca gcgagcaaga tcaggcgcgg
     6241 agaaatcccg aatgccccgc cagagcccca gatcaatcgg cgcgagacct tgatggacat
     6301 tctcgtgcaa ggaccaggcg agggaaaaga atacggattg aacagccttc cccaggcaca
     6361 aaggcgcttc ctcgagcggc tccgctggga gggcttgacg cgatattcga gccggcgcga
     6421 agccggcagc gccatcgaat cgatgcttga ctggttctgt gcgctgaacg gaaaaagcgc
     6481 ccacgatttc gagttccgcc tgcaggagtt ggagcgccgc tcccgctttc aggaaagaac
     6541 ccggcgcttc atggccgcag cggccaggac ccggtgcgac ttcacgaatc tgggaggaat
     6601 agacgtcgaa ctttgcaaca tcgttggata tcacccccaa ttccgtctta gcgacttacc
     6661 ccaagcgcag gtgaatgcag cggagttggc gaacaggcgt gaaatgtaag cagattttgc
     6721 cgtcgccgct gtgtgacttc aatccaactg ccttgccgca gtcgagtcag cgccgattta
     6781 cagtgcccgg cctttcagcg atgattggga cggccccgct ttacgattag gtgggaactc
     6841 tttcccccaa ctacactttt gtcgcctcat ggccgctgtc gatcccgttg ccgcagaagc
     6901 tccccccacg gccttgccgg ggttggcacg ggcgttgatt tccgccggca agctcggaca
     6961 aaagtcggcc gaagagattt cccgcaaggc ccaggccaac cgtaacagtt tcatcgcgga
     7021 gctgaccggc tcgggcgcgg tttccgcgtc cgatctcgcc cataccatgt ccaccgcctt
     7081 cggcgcgcct ttgctcgacc tggccgccat cgacctgcag cgactgccga agaacctgct
     7141 ggataacaag atctgccagg acttccgggt ggtggtgctg agcaagcgca acaatcgcct
     7201 gatcgtcgcc accgccgatc cgtcggacca gcaggcgggc gagaaaatca agttcgcgac
     7261 ccagatgggc gtcgactgga tcatcgccga atacgacaag ctgaccaaga tggtggaggc
     7321 cggaaccgcc agcgccaacg agacgatgga caacatcatc ggcgaggaat tcgagttcga
     7381 cgaatcctcg atcgaagcga cgacttccgg cgatgacaat gacgcggcca catccgaagt
     7441 ggaggacgca ccggtcgtca agttcctgca caagatgctg ctggacgcgt tcagcatgcg
     7501 cgcttcggac ctccatttcg agccctacga aaccaattac cgagtccgct tccgcatcga
     7561 cggtgaactg cgggagatcg cttcgccgcc ggtggccatc aaggaaaagc tggcctcgcg
     7621 catcaaggtc atttcgcgca tggacatctc ggaaaagcgc gtgccgcagg acggcaagat
     7681 gaagctcaag atcggcccgg accgggtgat cgatttccgc gtcagcacct tgcccaccct
     7741 gttcggcgaa aagatcgtga tccgtatcct ggaccccagc agcgcgcggg tgggcatcga
     7801 ggcactgggc tacgagcccg atgagaaggc ccgcctgatg gcggcggtag gccggcccta
     7861 cggcatggtg ctggtgacgg gtcccaccgg ctcgggcaag acggtgtcgc tctacacctg
     7921 cctgaacctg ctgaacaagc cgggcgtgaa catctccacc gccgaggacc cgtccgaaat
     7981 caacatgccc ggcatcaacc aggtcaacgt caacgagaag gcaggcctca ccttcgccgc
     8041 ggcgctcaag tcgttcctgc gccaggaccc ggacgtgatc atggtcggcg aaatccggga
     8101 cctggaaacg gccgacatcg cgatcaaggc tgcccagacc ggccacctgg tgatgtccac
     8161 gctccacacg aacgacgccc ccaccacctt gacgcgcatg cgcaacatgg gcatcgcgcc
     8221 cttcaacatc gcatccagcg tcatcctgat cacggcccag cggctggcgc ggcggctctg
     8281 tcccaattgc aaggcgccgg ccgacatccc gcatgaaacc ctgctcgaag cggggttcgc
     8341 ggatgaggac gtcgacggca cctggacccc ttaccgaccg gtcggctgct cggcgtgcaa
     8401 caacggctac aagggccgcg tcggcatcta tcaggtgatg ccgatcagcg aggacatcca
     8461 gcgcatcatc ctggcggacg gcagcgccct ggaaattgcg gcgcaggccg cacgcgaggg
     8521 tgtcaggaac ctgcgccagt ccggcctgca caaggtcaaa ctgggcgtca cgtccctgga
     8581 agaggtgctg ggctgcacca acgtataagc aagtcagagg agtacccaca tggccatggc
     8641 cgctaccaag gaattcgtct tcgagtggga aggccgcgac cgcaacggca agcaggtgcg
     8701 cggagaaacc cgcgccgccg gcgagaacca ggtgcaggcc gcgctgcgcc gccagggcgt
     8761 ttcgcccacc aaggtcagga agcgccggat gcgctcgggc gcgaaaatca agcccaagga
     8821 catcgccatc ttcacgcggc agctcgccac catgatgaag gccggtgtgc cgctgttgca
     8881 gtccttcgac atcgtcggcc gcggcaaccc caacgccagc gtcaccaagc tgctcaacga
     8941 cgtgcgcacc gacgtggaaa cgggcacctc gctgtcggcc gctttccgca agtacccgct
     9001 gtacttcaac aacctgtact gcaacctcgt ggaggccggc gaggccgccg gtatcctgga
     9061 agccctgctg gaccggttgg cggtctacat ggagaaaacc gagggcatca agtcgaaaat
     9121 caagtcggcg ctgatgtacc cgatctcggt ggtcgtggtc gcgttcgtcg tggtggcggt
     9181 gatcatgatc ttcgtgattc cctccttcaa ggaagtgttc gcctcgttcg gagccgactt
     9241 gcctgcgccc accctgatgg tgatcgcctt gagcgaattc ttcgtcgcct actggtggct
     9301 gattttcggc ggcatcggcg gtggcttcta ctttttcatg caggcctgga aacgcagcga
     9361 aaaagtgcag atgttcatgg accgggtgct gctcaaagtg cccgtcttcg gcgacctggt
     9421 ttacaagtcg gtgatcgcgc gttggacgcg caccctgtcc accatgttcg ccgccggcgt
     9481 gccgctggtg gaggcactcg actccgtggg aggggcgtcg ggtaactccg tctacgcctt
     9541 ggcgaccgag aagatccaga tggaagtctc caccggtacc agcctcacgg cggccatgac
     9601 caacgccggc gtgtttccca gcatggtgct gcagatgtgc gccatcggcg aagaatcggg
     9661 ctcgctcgac catatgctgg gcaaggcagc cgacttctat gaggccgaag tcgacgacat
     9721 ggtggccggc ctgtccagcc tgatggaacc gatcatcatc gtgttcctgg gcaccctgat
     9781 cggcggcatc gtgatatcca tgtacctgcc catcttcaag ctcggccaag tcgtctgatg
     9841 attttttcgc ccgtgatcga tgccgccctg gccggcgtgc tcggactgct cgtgggcagc
     9901 ttcctgaacg tcgtgatcta ccgcctgccc caaatgctgg agcggcagtg ggcggcggag
     9961 tgcgcggagc tggcgggcaa gccggcgcag gaagcgccgg ccttcaatct ggtcagtccc
    10021 cgttcgcgct gccggcagtg cggccacctg atccgatggt acgaaaacgt gccgatcctg
    10081 agctacctgg tgctgcgtgg caagtgctcg gcctgcggca ccgcgatcgg cttgcgctat
    10141 cccctggtgg aactggccac ggccatcctg ttcttctttt cggtccggca ctggggggcc
    10201 acgccggcag gagccgcctg gtgcggcttt tccgcagcca tcgtggccct ggccctgatc
    10261 gactgggaca ccacgttgct gccggacgac atcacgctgc ccttgttgtg ggcggggctg
    10321 atcgtcgccg cgctgcgctg gaccgacgtc acgctgccgc aggcgctgtg gggcgcggtg
    10381 gcgggctatg tctctttgtg gctggtctac cagggcttca agcttctgac cggcaaggaa
    10441 ggcatgggtt acggggattt caaactgttc gcggccctgg gagcctggtt tggatggacg
    10501 gcgctggtcc ccatcatcct gatggcttcg gtgatcggcg ccgtggtcgg catcgcgatg
    10561 aagttgtcca gcggcctgcg cgagggcggc tacgtgccgt tcggcccgtt tctggcgggc
    10621 gccggcctga cggcgatggt cttcggcccc gcctccatcc tgggcgtgat cgggctctag
    10681 catgtgggca tccgatgcgc catccaggcg cggcccgagg ggcgcctgat ggccgcgata
    10741 cgagtgggcc tgaccggcgg catcggcagc ggcaagagca ccgttctgca gatgctggag
    10801 cggctcgggg cggcggccat cgacgccgat gcgatttcgc gccgcaccac ggcggccggc
    10861 ggcgccgcca tcccggccat cgcggcgcgt ttcggcaacg aatttctcac gcccgagggt
    10921 gccctcgacc gggaccgcat gcgcgagcac gcttatgcgg acccgggggc ccgcatactg
    10981 ctggaagaga tcattcaccc gctggtgggc gcggagagca cgcgccaggt ccaggccgcg
    11041 gtcgccgccg gcgtccgatg catcgtgttc gacatcccct tgctggtcga atcgggccgg
    11101 tggcgagcgc aggtggaccg ggtgctggtg gtcgattgct cgccgcggac gcaggtagac
    11161 cgggtcgtcg cccgcagcgg ccgcacgccc gaggaagtgc ttgcgatcgt cgcggtgcaa
    11221 gccacccgcg gccagcggct ggcggccgcg gacctcgtca tctgcaacga ggggatgtcg
    11281 ctggaacagc tgcgtgacaa ggtggtacag acagcgcgcc gcttcgggct atgattggtc
    11341 gctttgccga cgagtccagt gtggacaagc cccagcgtga tcctctacga atatcccttc
    11401 aacgaacgca tccgcaccta cctgcgactg gagcacctgt tccgccgcct cggcgagctg
    11461 gtcccgcgtt cccatcccat tgatcaccat tacgcgctca cgaccatctt cgaagtgatg
    11521 gacgtggcgg cccgagccga cctgaagtcg gacgtgatga aagacctgga aaagcaaaag
    11581 cagctgctca acggctaccg cggcaatccc gccatcgccg aaggcgtgct ggacgaggtt
    11641 gtcgcgaagc tcgaacggtg cttcgccacg ctgaacaatc tgccgggcaa ggccggccag
    11701 tcgctgaccg agaacgaatg gctcatgagc atccgcagcc gggtcgggat ccctggaggc
    11761 acgtgcgagt tcgacctgcc ggcgtactac gcgtggcagc atcggactgc ggacaaacgc
    11821 cggaccgacc tcgagcgctg ggcgaacacg ctcgcaccgc tggccgagtc gatccacctg
    11881 ctgctcaaat tgctgcgcga ctcgggcacc ccccaaaaag tcatggccag ccacggccag
    11941 ctgcagcaga ccctgccgca gggccgcact tttcagctgc tgcgcctgcg gctcgatcct
    12001 gccctcggcc tggtgcctga aatcagcggc aaccgcctga tggtctcggt gcgcctgatg
    12061 cgccaggaat ccgccgaccg gctgcacgcg agcaacgaca gcgcccagtt cgaactcacc
    12121 ctctgttcct gaaggcgcgc cttcatggcc aggccgcacc cgccctccga cgcaaatccg
    12181 cgcctcgtgc ggtgcccggc ttgcggcggc gagagcgttt atgcgccggc gaatctttac
    12241 cggcccttct gttcggaacg ctgcaagaac atggacttgg gcgcctgggc cagcgaggag
    12301 ttccgggtgc cggaaaagcc cgcgcctgat gacgaatgac ttcggccgcg ccggagatga
    12361 caaatgacca ggtggcattc cggattcgtc atttgtcatt gaagcgaagc gacatcattt
    12421 gtcatgcgtt tcaccggcat acccccgctc ctgcgcgaac cactgcagca ccggcaccgt
    12481 gcccggcagc acgggccgca cctgaaccgg caggttctgc caggcgaacg actggccctc
    12541 gtgcatatgc agccggcccg tccactcgaa gaccttgcag aagttcagcc ggaccagcgc
    12601 atgcgggtaa tcgacacgct cgacgcgcca gggatgaacg ggaccaatgc ggagcccgat
    12661 ttcctcgagc agttcccggc gcagcgcctg ctcgaccgat tcgccgggtt cgaccttgcc
    12721 gcccggaaac tcccaatagc cttcgtaaac cttgccggtc gggcgagatg tgagcaggaa
    12781 gtcgccatcc gcgcggatca gcacgccgac ggccacctcg atctccttgc gatcgggccc
    12841 accttcacgc gggcggtcgc cgtcggcgac aagcggaccc ggtttcatgc cgggtggcgg
    12901 cccgcgtagt cccgtgcgaa ctgataggcc acgcggccac tgcgcgagcc gcgctccagc
    12961 gcccacacca gggcctcggg ccgggctgct tcgatggccg tcccatccac gccgaacgag
    13021 gacagccatt gcgccgtgat cgcaaggtac tcgtcctggc tgaacgggta gaaacttacc
    13081 caaagcccga aacgttcgga cagggagatc ttctcttcca ccacctcgcc cggatggacc
    13141 tcgccatcgg gcgtatgcgt gtagctcagg ttctccttca tgtactcggg cagcaggtgg
    13201 cgccggttgc tggtcgcgta gatcagcacg ttgggcgtgg cggccgccac cgagccatcg
    13261 agaatggact tgagcgcctt gaagccgggc tcgccttcct cgaagctcag gtcgtcgcag
    13321 aacacgataa atttctcggg ctgcgatgcc acgacgtcca cgatgtcggg caggtccacc
    13381 atgtcggcct tgtccacctc gatgagacgc aaaccccggc cggcgtattc gttgaggcag
    13441 gccttgatca gagaggactt gccggtgccg cgcgcgcctg tcagcaacac gttgttggcg
    13501 ggctggccct gcacgaactg caacgtgttg cgctggatct tctccttttg cgtgtcgatt
    13561 tccttcaggt cggcgagcct gatggcggcc cggtggcgca ccggctccag cgcgccatgc
    13621 ccgctcgacc gcttgcggta gcgccaggcc atcgacgccc tccagtcggg cgatgacagc
    13681 ggctgcggca ggatggattc gatgcgcgac agcaagtggc cggcgcgctc gatcagctgc
    13741 tcgaattttt cgttcaatct ctttccttcc cagggccccc ttggcgttcg ccccgcgcaa
    13801 ggggcgacat gctaagagcg gtaatcggca ttgatgctca cgtaatcgtg gctcaggtcg
    13861 caggtccaga ccgtgtcggc tgcgctgccc cgtcccagcc ccacccgcac ggtaatctcg
    13921 ccttgcttca tgacgcgctg gccgtcctcc tctcggtagg ccggattgcg cccgccgtga
    13981 acggccacgt gcacatcgtc caggtggagg tcgatccggg tctggtcgag gtcgtcgatg
    14041 ccggcgtagc ccaccgccgc caggatgcgg cccaggttgg gatcgctcgc gaagaaagcg
    14101 gtcttgacca ggggggaatg ggcaatcgca tacgccacct tccggcattc ctgcgctgtc
    14161 ttgccgcctt cgatgcgcaa ggtgatgaac ttggtggcgc cttcgccgtc gcgcacgatg
    14221 gcctgggcca gctggcgcgc gacgtcgagc atcgccgcct tcaacgcctg gccggacgcg
    14281 ctgtccagcg aagtaatggt cgcatgggcg gccttgttgg tggcgatcac cacgaacgaa
    14341 tcattggtcg acgtgtcgcc atcgaccgtc acccggttga acgagccttc ggcaagctcc
    14401 agggcaagct gccgcatcac ggcctggtcg agcctggcgt cgcaagccag gaagcccagc
    14461 atggtcgcca tgttcggccg gatcatgccg gcgcccttgc tgatgcccgt gatggtcacg
    14521 ggtgcgccct cgatcaccac ccggcggctg aaggccttcg gcacggtgtc ggtggtcatg
    14581 atcgcttcgg ccgcgcgcag ccagttgtcc ggcccggcgt cggccaacgc ggcgggcagg
    14641 ccggcctcga tccgctcgac cggcagcggc tccatgatca cgccggtgga aaacggcagc
    14701 acctgttccg gggcgatttc aagccgccgc gccagcgcga tgcaactggt gcgggcgcgc
    14761 accaagccat cctcgccggt gccggcattg gcgttgccgg tattgatgac gagggcacgg
    14821 acatcgccgc gcgcgagatg atcgcggcac acctgcaccg gcgcggcgca gaaacggttc
    14881 tgggtgaaca cgccggccac ggacgctccc gggtcgatca atacgaccgt caggtccttg
    14941 cgatcggctt tgcggatgcc cgcctcggcc acgccgacac gaacgccggg tacgggatgc
    15001 agatcagcgg cgcggggcgc gggcagattg acggccattg tcttgctcag gttcgagtgg
    15061 ttaagaaagt cggtgcccgg ttcagccctg gcgccacggc aggccgtgga agcgccagcc
    15121 gccgcgcttt ccgcgttgag ctttgtcgtc tggctctccc tcgaaccctt ccaggatgtt
    15181 ataggcctcg atgcccagct cggtcgctcg cctggcagcc gatatcgagc gcacgccgct
    15241 gcggcagaac agcacggctt tcctgcccgg cggcacggcc gccctgatgc cgtcgtcgaa
    15301 ggcactgttg attgccatgc ccggccactg cttccaggcc agcggcacgg ccccgggcac
    15361 gaaacccacc cattcgcgct ctgcgtcggt gcgcacgtcc accagcacgg cttcgccctt
    15421 ttgccaccag tcgaacgcca gctgtggcat gacgtcgccc gcatatccct cggctgggcg
    15481 gggcttgcct gaaccggtgg cctgcgcact ggcgccgggt gcgcccgcgt cgtgacgcag
    15541 gcccgagctc aggttggcgg gtacggcctc atcgatgcgc ttgggtttgg gcaggtgcaa
    15601 ttcgtccatg atcgccttga attgcgccag cgtcttcccg gccacgcggg cgtttccggt
    15661 tttctcggcc ccgatggtgg agtggctgcg cccgttgtag tcgtgggcgg gccagacggt
    15721 ggtggcgtcc ggcaggccga agagtatttc ggtgatgctc cggtacagcg cggcggcatt
    15781 gcccgactgg aaatcagtgc ggccgcagcc gttgatcagg agagcgtccc cggtgaatac
    15841 gcggtcgcgc cacagataac tcatgctgcc tgcggtgtgg cccggcgtgt gcaatgcctt
    15901 cagctgctcg acgccgaagc gcagcacatc gccgtgcttg agctgcactg ccgccgtccc
    15961 gatgccgcag ccttccggcg ccgccgtacg cgccccggcg tgctccgcga gcaggccggc
    16021 gctggtaatg tgatccgcgt gggcatgggt ttccaccgtc cacatcagtt tgaggtcgtg
    16081 ttcgcgcagc acttcgaggt cgcgcgccag ctgttcatcg acggggtcga tgatgattgc
    16141 ctcgcgggtg ttgtcgtcaa atacgacgta ggtatagctg ctcgaggccg ggtcgaacag
    16201 ctggatcggc tggttcatgg cgtcctcctg tgtcaggcat agtgtaaaac ctcgacacgg
    16261 gacgactggc cgtgcagtgc cgcaggccgt caggtgtccg gctcgttgcg agcatgccag
    16321 aggtcgacca gcatcaaggc gatgccgatc agggccacga cgaccatcga tatttccttg
    16381 accttgatga cgaaactcgt caggaaggcg accatcaaga ttgccccgag caaaccaaag
    16441 accagcttca tctgcgaatt ctcctcaagc tttgtcgctt tgtggccggt ccagccgccg
    16501 gaccagccag cgcgcggtgc gcagcagccg tgcatcgtcg cccttgccac caacgagctg
    16561 aacacccagc ggcaaaccgt tgacgccgct cagcagcgga aggctgattg ccggcaggcc
    16621 tgtcagtgtc cacagcgtgc acatcagggg atcgccggtg ccttccagac cgcccggtgc
    16681 cgttccgagc gtagccggcg tgacgatggc gtcgaactcg gcgaacagtt catcgagata
    16741 cgccaccaac gaggggatgc gctcaaccgc atgccggtaa tcgacggcgc tgacctggag
    16801 gccgcgctcg atctgcgcgc acaaagacgg ggaaagccgc tcgcggccgg atttgaactc
    16861 ccgcgcgaat gacgcggcca ggtcggcctc catgatggtg cggtgccagg ttaccgcctc
    16921 ggccgcggaa tcgtgtagct cgaccggcat tccggcgtcg cccagctcct ctgcaaggtc
    16981 ctcgaaggcc tcgcgggcgt cggcatccat gcgctcccag aaaggcgtgc gcaccacggc
    17041 gaagcgcggc ggcaggggcg gctgcgacgc gaccagcttg cacatcggcg gggcaggctg
    17101 ggcccgcgtg gccgggtcgt cctcatcgtg gccgcacaga gcctgcgcca gcagggcgat
    17161 gtcttccagg ctgcgcgcaa aaacaccgac ttggtcaagc ggcggcgatt gcgtcagcac
    17221 acccgttcgt ggaatgaggc caaagctcgg cttgaacccg taaacgccgc aaaaagaggc
    17281 cggccgaagc accgagccat tggtctgcgt gccgaccgcc atcgcaacca tgcccgcggc
    17341 cacggccgca gccgacccac tggacgagcc accgggcgtg tgttcgcgtc cggctaagtc
    17401 ccgatgagga tttcgcgtct tgccgggcgc ataggtggcc agttcggtgg tcacggtctt
    17461 gcccatcggg atgcccccgg cgcgcctcag ctgacgcacc agggccgcgt cgcttcgagg
    17521 cattcgaccg gcatgcaaag gcgttccatc gccggtgggc atgccccgaa cgtcgatgat
    17581 gtccttgatc ccgaccggaa tgcctgaaag cggccctgtc tcggctccgc gctggtgccg
    17641 aagatcggca tcggcagcct gttcggtgag gtattcacga tccaggaact cccaggcctg
    17701 tacctgcggc tcgacttcgt cgacacgcgc cagcagcgcc cgtgcgtagt cttcggccga
    17761 acattcgccc tcccgcaggc gccgggccat ctcggttgca ctcagcagat acaaagacaa
    17821 agcgccctcc tgttgtgcgg gttttaagtc aacgtccgta catcaggccg ggcagccagg
    17881 tcacgaaccc cgggaccacg tacatcgcga tcatcgccac gaacaccatt cccaggaagg
    17941 gcatgcagcc gcggaagatc gagctcagca ggacatgcgc cggggcgacg cccttcagat
    18001 aataggcgga catggccatc ggcggagtca ggaacgaggt ctgcaggttc agcgccacca
    18061 ggatgccgaa gaagatcggg tcgacgttga agtgcggcag caacggcagg aagatcggca
    18121 cgaagatgat gatgatctcg ctccactcca gcggccagcc cagcaggaag atgatgacct
    18181 gcgagagcag caggaaggtg acggtgttca ggcccatgcc cagcacgaac tccttgatca
    18241 gcgcctcgcc ccccaggtag gagaagatcg atgagaacag ccaggagccg atgaacaggt
    18301 agcacaccat ggcactggtg cgcgccgtga ggaacaccgc ctcgcgcagg cgctgccagg
    18361 tgaacgcctg gtagccgacg gcgagcacca cggatcccaa cgccccgatc gcggccgcct
    18421 cgctgggcgt tgccagcccg aacagaatga cgccgagcac cgacatgatc aggaacgcca
    18481 gcggaaagaa agccttgagc atgagcgcca ccaccgccag gaaatccatc cggtgctggt
    18541 cctccgccag tttgggcatc aggccgggat tcagcagggc gcgaaacacg acgtagatca
    18601 tgtacaggcc tgccaggaag aagcccggca gcagggcggc cgcgtacagc ctgaccaccg
    18661 aaacgccggc agtggctcca tacacgatca gcatgatcga ggggggaatc aggatgccca
    18721 gcgttccgcc ggcgcagat
//
LOCUS       DASWIY010000459         4947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2921102, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000459 DASWIY010000000
VERSION     DASWIY010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2921102"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VGA59_16545"
     CDS             <1..250
                     /locus_tag="VGA59_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9721328.1"
                     /translation="SKAKGAADAASVHCETLLREVTGKRIADVIVEQAQQHACDLIVM
                     GTHGRKGFTRLTLGSAAEGVARSSPVPVLLVRLEDAKP"
     gene            complement(319..462)
                     /locus_tag="VGA59_16550"
     CDS             complement(319..462)
                     /locus_tag="VGA59_16550"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9721329.1"
                     /translation="MRSNVSRTFSLAEDVDDSKAEAKYADGVLTLELPKKATAASRKL
                     TVN"
     gene            complement(502..1155)
                     /locus_tag="VGA59_16555"
     CDS             complement(502..1155)
                     /locus_tag="VGA59_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX9721330.1"
                     /translation="MKTDAQLKADVTDELTWDAAVKATGVGVMVENGVVTLTGHLDSF
                     AEKHAIEHAVRRAAGVRGIALELDVKAASEHRRSDSEIARAAAEAIRLNSLVPEGKVR
                     VEVENGWVTLTGDVDWGYQFASAEQCVRPLAGVRGLFNEIAVTPRVKGDDIATQITAA
                     LTRQAVREASHIGIEVEGGVVTLSGKVHSRAEHDAALGVAFAARGVSHVVDRLRIGG"
     gene            1372..2166
                     /locus_tag="VGA59_16560"
     CDS             1372..2166
                     /locus_tag="VGA59_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEX9721331.1"
                     /translation="MALLLGWMCAHAWALDPVTDMAVLYSTEVDRRLVVPAAEVRRYA
                     RLAEEALARAQVTLSGPQYLAVVDRDPNTQALFLLFRDTAGRLELVGASPVSTGRAGA
                     FDHFETPTGVFDHNLANPDFRTEGTPDGEGLRGYGVKGMRVYDFGWQKVPKGWGDGVA
                     IRMRLQMHATDPDLLERRLGRAQSEGSIRIPGTLNRLIDRHGLLDADYRRLRDDHPLW
                     PPDAKREPVPSPGRYLIVVDTQRADRPDWNPAPFIPHRRPAAPIPR"
     gene            complement(2163..3152)
                     /locus_tag="VGA59_16565"
     CDS             complement(2163..3152)
                     /locus_tag="VGA59_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX9721332.1"
                     /translation="MRAMTMAGPAGQLVAASKPLPQALGHDVVVRVLACGVCRTDLHI
                     VDGELPPRRPAIVPGHEVVGHVVARGPQAKRFAIGQRIGIPWLGRTCGHCGFCAMDRE
                     NLCDHPVFTGYDRDGGFAEYMAADDRFCFALPDRYDDAHAAPLLCAGLIGFRAWRLGG
                     AAAPRRLGLYGFGAAAHIICQVAVARGQEVYAFTRAGDSEAQAFARSLGAVWAGGSDT
                     APPEALDAALIFAPVGSLVPTALGGVRKGGIVVCAGIHMSTIPAFDYRLLWGERVLKS
                     VANLTRHDGEAFFNLVAELPLQTHVRTFALADANEAIHAVREGRVSGAAVLIP"
     gene            3267..>4947
                     /locus_tag="VGA59_16570"
     CDS             3267..>4947
                     /locus_tag="VGA59_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9721333.1"
                     /translation="MSNSGTRPAVHGPAPMDLPLKLSLARLAHGISPASVAMAYSDWF
                     THLLVSPSKHAELAGSALRNSVLWQQYWANAWRGDCHPCVEPQPEDKRFKPREWDAPP
                     FNAMAQVFLLQDQWWNEAMTGVRGVSRHHEEMAAFITRQLLDVWSPSNFIATNPQVLK
                     QTLRMGGINLASGFANWARDAMAVLGEGKPRGVEEFAPGEGVALTPGKVVFRNRLIEL
                     IRYEPVTPKVHAEPVLIVPAWIMKYYILDLTPDDSLVKYLVGEGHTVFIVSWANPGSE
                     GRDLGMDDYLELGAIAAVKAVQSQCPGAGIHAVGYCLGGTMMAIAASVLGAGSRNSLK
                     TVTLLAAQVDFHEPGELGLFIDESQISFLEDLMAERGYLDGRQMAGAFALINSKDLVW
                     SKLVHEYLMGAQTPMAALRAWNADATRLPARMHSEYLRRLYLHNDLAEGRYPVGGRAI
                     SLRDIKVPLFVVATERDHVSPWPSVYKIQRLTHAPVSFLLTSGGHNVGIVNPPSGPMV
                     HPQASYRFASHGPHKAPADPQAWLEAAPSFDGSWWPCWQQWLIEHSGGRIAA"
BASE COUNT          816 a         1680 c         1626 g          825 t
ORIGIN      
        1 gtccaaggcc aagggtgcgg ccgacgccgc ctccgtgcat tgcgaaacat tgctgcggga
       61 agtgacaggc aagcgcattg cggacgtgat cgtcgagcag gcccagcagc acgcgtgcga
      121 cctgatcgtc atgggcacgc atgggcgcaa gggattcacc cggctcacgc tgggcagcgc
      181 ggccgaaggc gtggcgcgta gcagcccggt gccggtgctg ctggtgcggc tggaggacgc
      241 gaagccctag cgaaacgcgt ggccgaagca ccggcgctgg cgtttcgctg cgccggtgcg
      301 ggagtcatgc ggccagcctc agttgacggt cagtttcctg gaagccgccg tcgccttctt
      361 gggcagctcc agcgtcagca cgccatccgc atacttcgcc tcggccttgc tgtcgtccac
      421 atcctcagcc aggctgaagg tacgcgaaac gttgctcctc actgattgat gaagaagatc
      481 caccttcgcc gcggcagcct ttcagcctcc gatgcgaagg cggtccacga cgtgcgacac
      541 gccgcgcgcg gcgaaggcca cgccgagggc ggcgtcatgc tccgcccgag agtgcacctt
      601 gccgctgagc gtgacaacgc cgccctccac ctcgatgccg atgtggctgg cctcacgcac
      661 cgcctgccgc gtgagcgcag ccgtgatctg cgtggcgatg tcgtcaccct tcacgcgggg
      721 cgtgacggcg atctcgttga acaggccgcg cacgccggcc agcggccgca cgcattgctc
      781 ggcgctcgcg aactggtacc cccagtcgac gtcgccggtg agcgtcaccc agccgttctc
      841 cacctcgacc cggaccttgc cctcgggcac cagcgagttc aggcgaatcg cttcggcggc
      901 cgcccgggct atttcggagt cgctgcgccg gtgctcgctc gcggctttca cgtccagttc
      961 cagcgcgata ccgcgaacgc cggcggcgcg ccgcacggca tgctcgattg cgtgtttctc
     1021 ggcgaagctg tccagatggc cggtgagggt cacgacgccg ttctcgacca tcacgccgac
     1081 gccggtcgct ttgaccgccg catcccaggt cagttcgtcc gtgacgtcgg ctttaagttg
     1141 agcatctgtt ttcactgttg agactccaca agcaccgtcc tcgaggcggc tcgaatcaaa
     1201 tctaggtggc gagaggcaat ccgctttgac atgccgcaag tttttgccct gaacgcgatg
     1261 ttgattgacc tcgctcagtg ccaaagtcca catgcgaacc agaattggtt gatgggtgga
     1321 cagagaagaa gcctcatgat gcgggcacta tgcatatctc ttcgagtgac catggccttg
     1381 ctgttgggct ggatgtgtgc gcatgcatgg gctctcgatc cggtgaccga tatggccgtg
     1441 ctgtacagca cggaggtgga ccgcaggctc gtggttccgg ccgccgaggt gcgtcgctat
     1501 gcgcgcctgg ccgaggaagc gctggcccgg gcccaagtga cactttccgg gccgcagtac
     1561 ctcgcggtcg tcgaccgcga cccgaatacc caggcgctat tcctgctctt ccgcgacacc
     1621 gcaggccggc tggaactggt cggcgcatcc ccggtatcca ccgggcgtgc cggagcgttt
     1681 gaccatttcg aaacgcccac cggcgtgttc gaccataacc tggccaaccc ggacttccga
     1741 acggagggca cacccgacgg cgagggcctc aggggctacg gcgtcaaggg gatgcgggtc
     1801 tacgacttcg gctggcagaa ggtgcccaaa ggctggggcg acggcgttgc gatccggatg
     1861 cggctgcaaa tgcacgctac cgatcccgat ttgctggagc gccgcctggg cagggcgcag
     1921 tccgaaggtt ctattcgcat tcccggcacg ctgaaccggc tgatcgaccg tcacgggttg
     1981 ctggacgccg attaccggcg gctgcgcgac gaccacccgc tttggccccc cgatgcgaaa
     2041 cgcgaacccg ttccttctcc cggccgctac ctgatcgtgg tggacaccca gcgtgcggac
     2101 cggcccgatt ggaatccggc gccgttcatc ccacaccgca ggcccgccgc gccgattccg
     2161 cgctaaggga tcagcaccgc cgcgccgctc acccggccct cgcgcaccgc gtgaatcgcc
     2221 tcgttggcgt cggccagcgc gaaggtccgc acatgggtct gcaacggcag ctcagccacc
     2281 aggttgaaaa atgcctcgcc atcgtgtcgc gtgaggttgg ccaccgattt cagaactctc
     2341 tcgccccaga ggagccggta gtcgaaagcc gggatggtgc tcatgtggat gccggcgcag
     2401 accacgatgc cgccctttcg aacgccgccc agcgcagtcg gaaccaatga gcccacgggt
     2461 gcgaagatca gggccgcgtc gagcgcctcc ggcggcgccg tgtccgagcc gcccgcccag
     2521 acggcgccca ggctgcgcgc gaacgcctgg gcctcggagt cgccggctcg cgtgaacgca
     2581 tagacctcct ggccgcgcgc cacggcaacc tggcagatga tgtgggctgc cgcgccgaag
     2641 ccgtacaggc ccagccggcg cggcgcggcc gcgcctccca ggcgccacgc acgaaagccg
     2701 atgagccccg cgcagagcag cggcgcggcg tgcgcgtcgt cgtaacggtc gggcagggcg
     2761 aagcagaagc gatcgtcggc cgccatgtac tcggcgaatc cgccgtcgcg gtcgtacccg
     2821 gtgaacaccg ggtggtcgca gagattctcg cgatccatgg cgcagaaccc gcagtggccg
     2881 cacgtcctgc ccagccaggg gatgccgatg cgctggccga tggcgaaacg cttcgcctgc
     2941 ggcccgcgcg ccaccacgtg gcccaccacc tcatgtcccg gcacgatggc gggtcggcga
     3001 ggcggcagct cgccgtcgac gatgtgcagg tcggtgcgac aaaccccgca ggcgaggacg
     3061 cgcaccacca cgtcatgacc cagcgcctgc ggcagcggct tgctcgccgc caccagttga
     3121 ccggccgggc cggccatggt catcgcacgc atgaatgccg gcaacatggt tgaatggtgc
     3181 actctttagg gcgcgggggc gcggcattcg cgctgtcgtt gaccttgcgc aaaaaatccc
     3241 taccgagcag ttgagaccat acagccatgt cgaattccgg cacacgtccg gccgtccacg
     3301 ggccggctcc gatggacctg cccctcaagc tgagcctggc ccggctggcg catggcatct
     3361 cgccggcctc cgtggcgatg gcctattcgg actggttcac ccatctgctg gtttcgcctt
     3421 ccaagcacgc cgagctcgcg ggcagcgcgt tgcgaaactc cgtgctgtgg cagcaatact
     3481 gggccaatgc ctggcgcggc gattgccacc cctgtgtcga accgcagccc gaggacaagc
     3541 gcttcaagcc gcgcgaatgg gacgcgccgc cattcaatgc catggcccag gtcttcctgc
     3601 tgcaggatca atggtggaac gaggcgatga cgggcgtacg gggcgtgtcg aggcaccacg
     3661 aggaaatggc cgccttcatc acgcgccagt tgctggatgt ctggtcgccg tcgaatttca
     3721 tcgccaccaa tccgcaggtc ctcaagcaga cgctgaggat gggtgggatc aacctggcca
     3781 gtggtttcgc caactgggca cgcgacgcca tggccgtgct cggcgaaggc aagccgcgcg
     3841 gcgtcgagga attcgcgccc ggcgagggcg tggcgctgac gcccggcaag gtggtctttc
     3901 gcaatcgcct gatcgagctg atccggtacg agcccgtgac gccgaaggtc catgccgagc
     3961 cggtgctgat cgtcccggcg tggatcatga agtactacat cctcgacctg actcccgacg
     4021 attcgctggt caagtacctg gtgggcgagg gacacaccgt gttcatcgtg tcctgggcca
     4081 acccgggcag cgaagggcgc gacctgggca tggacgatta cctggaactg ggcgcgatcg
     4141 ccgccgtgaa ggcagtgcag tcgcaatgcc ccggggccgg catccatgcc gtgggctatt
     4201 gcctgggcgg caccatgatg gcgatcgcgg cttccgtgct gggcgccggt tcacgcaact
     4261 cgctcaagac cgtgaccctg ctggccgcac aggtcgattt ccacgagccg ggcgagctgg
     4321 ggctgttcat cgacgagagc cagatctcgt ttctggagga cctgatggcc gaacgcggct
     4381 atctcgacgg ccgccagatg gccggcgcat ttgccctgat caactccaag gacctggtgt
     4441 ggtccaagct cgtgcacgag tacctgatgg gtgcgcagac gccgatggcg gcgctgcgtg
     4501 catggaatgc cgacgccacg cgcctgccgg cgcgcatgca cagcgaatac ctgcgccggc
     4561 tctacctgca caacgacctg gccgaggggc gctatccggt gggcggacgg gccatctccc
     4621 tgcgcgacat caaagtgccg ctgttcgtgg tcgccaccga gcgtgaccat gtttcgcctt
     4681 ggccgtcggt gtacaagatc cagcgcctca cccacgcgcc ggtcagcttc ctgctgacct
     4741 cgggcggcca caacgtcggc atcgtcaatc ccccgtccgg tccgatggtg catccgcagg
     4801 cgagttatcg tttcgcttcg cacgggccgc acaaggcgcc cgccgatcca caggcctggc
     4861 tcgaagccgc gccttcattc gatggatcct ggtggccttg ctggcagcaa tggctgatcg
     4921 agcattccgg agggcggatt gcagccg
//
LOCUS       DASWIY010000460         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3618939, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000460 DASWIY010000000
VERSION     DASWIY010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3618939"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VGA59_16575"
     CDS             complement(<1..304)
                     /locus_tag="VGA59_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721334.1"
                     /translation="MIDFTASYPNSRKVYETRTVSLSPGGSVATIQVPVREVALSGGE
                     PPIRLYDTSGSIGHDVRDGLPKLRESWIAARRATGTVGTQLYYARRGEITPEMEFIA"
     gene            738..1382
                     /locus_tag="VGA59_16580"
     CDS             738..1382
                     /locus_tag="VGA59_16580"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX9721335.1"
                     /translation="MTNPLGATLAGIHVEIIGQSDRSGETNASGQISFTGMQAGTYRV
                     RCTGERVVWFEKEVTLRAGQTTNVDITLAAAPPPPEPPPPPAPTPVEPPPPPVGPAGE
                     PRSLSIVDLIERDLIQGNQPRKDTLVSCSGNTRSTLVQLNQDQPQRLYDNAEVTYYVV
                     AGEGALKMDGREIALEAGGFVSIPRGTGHAIGRRGRRPLILLATLSGTPCEQAR"
     gene            complement(1417..1806)
                     /locus_tag="VGA59_16585"
     CDS             complement(1417..1806)
                     /locus_tag="VGA59_16585"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9721336.1"
                     /translation="MAAYVDSSAIVKLAIREPESTALRRYLRRRGPLVSSALARTEVL
                     RALLPAGDEAVARGRSVLQRLDLVRVNDRVLNAAAVLRPPALRSLDAIHLATAQQLGP
                     DLTVLVSYDDRMVTSAKQLGYRIVQPR"
     gene            complement(1809..2084)
                     /locus_tag="VGA59_16590"
     CDS             complement(1809..2084)
                     /locus_tag="VGA59_16590"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9721337.1"
                     /translation="MASIGVRELRQRASELLRRVEQGETIEVTDRGRPVALLSPPPEG
                     SPLDRLRALGEIDPALGDLDDLPEPLVLAPGIEHPSEVLSRLRQDER"
     gene            complement(2126..2536)
                     /locus_tag="VGA59_16595"
     CDS             complement(2126..2536)
                     /locus_tag="VGA59_16595"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9721338.1"
                     /translation="MADQRHARGLIDTSVVVDLERIEPADLPPEIAVSAVTLAELAAG
                     PHATADPAERARRQDRLQRAEATFEPLPVDAAVARAYGRVYAAVAAAGRNARGRRAVN
                     LFIAATAVAAGLPLYTRNPDDFAGLSDLLEIVSV"
     gene            complement(2529..2783)
                     /locus_tag="VGA59_16600"
     CDS             complement(2529..2783)
                     /locus_tag="VGA59_16600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9721339.1"
                     /translation="MNREITQRELRNESGRIMRALDRGKSFIITRNGVPVGELIPLRQ
                     RVFVPAEAALAAFAGAPRVPLGRFRNDLDAVIDQDPTPRG"
BASE COUNT          490 a          977 c          925 g          422 t
ORIGIN      
        1 cggcgatgaa ctccatctcg ggcgtgatct cgccgcgccg ggcgtagtag agctgtgtgc
       61 ccaccgttcc ggtggcgcgg cgcgcggcaa tccacgattc gcgaagcttc gggaggccgt
      121 cgcggacatc gtgaccaatc gacccgctcg tatcgtagag gcggatcggc ggctcgccgc
      181 cgctgagcgc cacctcgcgc accggcacct ggatcgtggc aacgctgccg ccgggcgaca
      241 ggctgactgt ccgtgtttcg tagaccttcc gggaattcgg atacgacgct gtgaaatcga
      301 tcatgaactg ctgaaggcgg gaccgcgccg cgctccctcc gccggtttca accggatccg
      361 cctccgcaca cggcttcggc ggacccgccg tagcgcgtac gcgcgcgaag gcgggtcagg
      421 ttccaagggt ttgtctcaat cccgatggga tacccctggc ggccagtgac gattatactc
      481 gcgagatgcg cgggtggtcg ttggtggtta gctggttagt ggttagtggt tggctagggg
      541 gttctgcgct cgcaacagcc gagccaccca cgattcccgg acccctaacc actaactacc
      601 aaccagctaa ccaccaacca gctaaccacc aaccagcctt cgcgcaggcg cccgcgccgg
      661 ccccaaaacc agcggcgccc gcgccggccg cccggcgccc cgcggcgccg ccagcgcgcg
      721 cagggatggc cctgaccgtg acgaacccgc tgggcgccac tcttgccggc atccacgtgg
      781 aaatcatcgg ccagtctgac cgcagcggcg aaacgaacgc cagcggacaa atcagtttca
      841 ccggcatgca ggccgggaca taccgcgtgc gctgtaccgg ggaacgcgtg gtctggttcg
      901 agaaggaagt gacgcttcgc gccggccaga cgaccaacgt cgacatcacg ctggccgccg
      961 cgccgccgcc ccccgagccg cccccaccgc cggcgcccac accggtggag ccgccgccgc
     1021 cgcccgtggg accggctggc gagccgcgga gcctctcaat cgtcgatctc atcgagcgcg
     1081 acttgatcca gggcaaccag ccgcgaaagg acacgctggt gtcgtgcagc ggcaacacgc
     1141 gcagcacgct ggtgcagctc aaccaggacc aaccccagcg cctctacgac aacgccgaag
     1201 tcacctacta cgtcgtcgcc ggcgaagggg cgctcaagat ggacggccgc gagatcgccc
     1261 tcgaggcggg cggcttcgtc tccatcccgc gcggcaccgg ccacgccatc ggacgccgcg
     1321 gccgccggcc gctgatcctg ctcgccacgc tgagcggcac gccctgcgaa caggcgcgct
     1381 gacacattct aaaggtcgtt ctcgcgcgcc gggagatcag cgcggctgga cgatcctgta
     1441 gcccagttgt ttcgctgagg tgaccatcct gtcatcgtac gagacgagga ctgtcaggtc
     1501 gggaccgagc tgctgtgccg tggcgaggtg aatggcgtcc agcgagcgta acgcaggcgg
     1561 gcgcaagaca gctgcggcat tcaggacgcg atcgttcacg cgaacgagat ccagccgctg
     1621 aagcacgctg cgccctctgg caaccgcctc gtcacccgcc ggcaggagcg cgcgcaacac
     1681 ttccgtgcgc gcgagggcac tggataccag aggacctctc cgccggagat agcggcgcag
     1741 cgcggtagac tcgggctccc gtatcgccag cttcacgatg gctgaagagt cgacgtacgc
     1801 tgccacgatc agcgctcgtc ctgtcgcaga cgactgagca cttcggatgg atgttcgatc
     1861 cccggcgcaa ggaccagcgg ttcgggcaag tcatcgagat cgcccagcgc agggtcgatc
     1921 tcacccaagg cgcgcagccg atcgagcggg ctgccctcgg gcggcggcga gagaagcgcg
     1981 accgggcgcc ctcgatccgt gacctcaatc gtttcccctt gctcgacgcg ccgcagcagc
     2041 tcgctggccc tctgtctgag ctcccgaacc ccgatggatg ccatgtgcta cttatagcac
     2101 accgtatcgc ggacgcgcca gcggactaaa ccgagacgat ttcaagcagg tccgacaggc
     2161 cggcgaaatc gtccggattg cgcgtgtaga gaggcagacc cgccgcgacc gcggtcgcag
     2221 caatgaacag attcaccgcg cgccggccgc gcgcgttgcg gcctgccgca gcgaccgccg
     2281 cgtagacgcg gccgtatgca cgggccacgg ctgcatccac cggcagcggc tcgaacgtgg
     2341 cttcggcccg ctgcaaccgg tcctggcgac gggcgcgttc tgccggatcg gcggtcgcat
     2401 gcggtcctgc cgcgagttcg gccagcgtca ccgcagagac ggcgatctcc ggaggcaaat
     2461 cggccggttc gatccgctcc aggtcgacga caacggaggt gtcgatgagt ccgcgggcat
     2521 gacgttggtc agccacgcgg cgtggggtcc tgatcgatga cggcgtcgag atcgttccgg
     2581 aaccggccga gtggtacgcg tggcgcgccg gcaaaggcgg ccaaggcagc ttcggctggt
     2641 acgaacaccc gctgtcgcaa gggaatcagc tcgccgacag gcacgccatt gcgtgtgatg
     2701 atgaaggact tcccccggtc cagtgcacgc atgattcgcc cgctttcgtt gcggagctcc
     2761 cgctgggtga tctctcggtt cataaccggg tgattgtagc acaagtgcta cggg
//
LOCUS       DASWIY010000461         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_314702, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000461 DASWIY010000000
VERSION     DASWIY010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314702"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1903)
                     /locus_tag="VGA59_16605"
     CDS             complement(<1..1903)
                     /locus_tag="VGA59_16605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX9721340.1"
                     /translation="MSTLTETPTRKREVIEKAVIRFAGDSGDGMQITGSQFTNTVAMY
                     GNDLATFPDYPAEIRAPAGTLPGVSGYQVHFASTDVYTPGDAVDVLIAMNPAALKVNL
                     PDLKANGILIVNTDNFKDNDLRKAHMTANPLEDHSLDAFRLFPVELSRLTRLALEDLG
                     LDTKSMDRCKNFFALGMCYWLYNRALDSTFRWLEDKFKAKPLLVEANKLAMKAGYAYC
                     EATEAFQVSYEIPPAKLSPGVYRNISGNTALALGFVAASQKSGLPLFLGSYPITPASD
                     ILHELSALKDFGVTTFQAEDEIAAVTSAIGAAYGGALAITTTSGPGMALKTEAMGLAT
                     MVELPIVICDIQRGGPSTGLPTKTEQADLLQAVFGRNSEAPIPVLAASTPGDCFWVAL
                     EAARIAVKYMVPVIILSDGYLANGAEPWRIPRVEDLPEIPVKFATDPNGFQPYRRNPD
                     TLARPWAVPGTPGLEHRIGGLEKQDVTGNVNYEPMNHERMVHLRAAKVEAIVQDVPDV
                     APAGDPDGDLLIVAWGSTYGSVTAALNSMRERGHRIGHVHLRHLNPLPANLGEVMQRY
                     KSVLVPEMNMGQLVVLLRAKYLVDAQGYNKIQGKPFKQSELEAKIEEMLGLRPPMKVR
                     HRPEPSVEVG"
BASE COUNT          308 a          587 c          651 g          364 t
ORIGIN      
        1 ggcccacttc cacggacggt tcgggccggt ggcgcacctt catgggaggc cgcaggccca
       61 gcatctcttc aattttggct tccagttcag attgtttgaa cggcttgccc tggattttgt
      121 tgtagccttg cgcgtccacc aggtacttgg cgcgcagcag gaccaccaac tggcccatgt
      181 tcatctccgg caccagcacg ctcttgtagc gctgcatcac ttcgcccagg ttggcgggca
      241 gcgggttcag atgcctcagg tggacatgcc caatgcggtg gccgcgctcg cgcatggagt
      301 tcagcgccgc agtcaccgaa ccgtaggtcg agccccaggc gacaatgagt aaatcgccgt
      361 cggggtcgcc ggcgggcgcc acgtcgggaa cgtcctggac gatggcctcg accttggccg
      421 ctcgcagatg caccatacgt tcgtggttca tgggttcgta gttgacgttg ccggtgacgt
      481 cctgcttctc caggccgccg atgcggtgct cgaggcccgg cgtgccgggc acggcccacg
      541 gccgcgccag cgtgtcggga ttgcggcggt agggctggaa gccgttgggg tcggtggcga
      601 acttcaccgg aatctccggc aagtcttcga cccgcgggat gcgccacggc tccgcgccgt
      661 tggccaggta accgtcgctc aggataatga ctggcaccat gtacttcaca gcgatgcgcg
      721 ctgcctccag cgcgacccag aagcagtcgc ccggcgtgga cgcggccagc acgggaatgg
      781 gcgcttccga gttgcgtccg aagacggcct gcagcaaatc ggcctgctcg gttttcgtcg
      841 gcaggccggt ggagggcccg ccgcgctgga tgtcgcaaat cacgatgggc agctcgacca
      901 tggtggccag gcccatggcc tcggtcttca gcgccatgcc cggccccgac gtggtggtga
      961 tggccagcgc gccaccgtag gccgcgccaa tggccgaggt gaccgcggcg atttcgtctt
     1021 cagcctggaa ggtggttaca ccgaagtcct tcagggccga aagctcgtgg aggatgtcgg
     1081 aggccggcgt aatcgggtag gagcccagga acagcggcag gcccgacttc tgcgacgcgg
     1141 ccacaaagcc cagggccagc gccgtattgc cactgatgtt gcggtacacc cccggtgaca
     1201 gcttggccgg cggaatctcg tagctcacct ggaacgcctc ggtggcttcg cagtaggcgt
     1261 aacccgcctt catggccagc ttgttggctt ccaccagcag cggtttggcc ttgaacttgt
     1321 cttccagcca gcggaaggtg gagtccaggg cgcggttgta gagccagtag cacatgccca
     1381 gcgcgaaaaa gttcttgcag cggtccatgg acttggtgtc caggcccaga tcttccagcg
     1441 ccaggcgcgt caggcggctc agctcgacgg ggaacaggcg gaaggcgtcg agcgaatggt
     1501 cctccagcgg attcgccgtc atgtgcgcct tgcgcaggtc gttgtctttg aagttgtcgg
     1561 tgttgacgat caggatgccg ttggccttca agtccggcag gttgaccttc agcgccgcgg
     1621 ggttcatggc gatcagcacg tccacggcgt cgcctggggt gtaaacgtcg gtggaggcaa
     1681 agtgcacctg gtagccgctc acgccgggca gcgtgccggc gggggcgcgg atctccgcgg
     1741 ggtaatcggg gaaggtggcc aggtcgttgc cgtacatcgc gacggtgttg gtgaactggc
     1801 tccccgtaat ctgcatgccg tcgccggaat cgccggcaaa acggatgact gccttttcaa
     1861 tgacctcgcg cttgcgagtg ggagtttcgg taagagtgct cattcctctg
//
LOCUS       DASWIY010000462         4728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1440149, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000462 DASWIY010000000
VERSION     DASWIY010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440149"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /gene="oxc"
                     /locus_tag="VGA59_16610"
     CDS             complement(<1..1380)
                     /gene="oxc"
                     /locus_tag="VGA59_16610"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEX9721341.1"
                     /translation="MTTDARSQAVADGFHLVIDALKLNGIDTIFGLPGIPITDLTRLA
                     QAAGMRVISFRHEQNAGNAAAIAGFMTQKPGICLTVSAPGFLNGLTALANATTNCFPM
                     ILISGSSEREIVDLQQGDYEEMDQLAIAKPLAKAAFRVLHVEDIGVGIARAIRAAVSG
                     RPGGVYLDLPGKIFAQTMDAEAGRKSLIKVVDPAPRQIPAPDAVKRALDLLKSARRPL
                     IVLGKGAAYAQADADIQALIEKTGIPYLPMSMAKGLLPDTHEHSAAAARSYVLPEADV
                     VMLIGARLNWLLSHGKGKTWGGKDHKAWGGQRFIQVDISPQEADSNVRIDAPVVGDIG
                     SCVAALLAGIGFNWSRPSAEWLGAIAERKNKNIARMAETLVKDPKPMNFHSALNVVRD
                     IVKANPDAILVNEGANALDFTRSIVDMYKPRKRLDVGTWGVMGIGMGFSVAAAVVSGQ
                     PVIAIEGDSA"
     gene            1721..2329
                     /locus_tag="VGA59_16615"
     CDS             1721..2329
                     /locus_tag="VGA59_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9721342.1"
                     /translation="MPLLSQHPEARIVRVQLRQNLILKHLDAGQWEQIETLLEVIDYA
                     KNEALELQGALSMEQYFIIDGILKRVVSNAQGKEMILRFAEESDIDTSYAAWRLKRPV
                     PYSIRAVTKVRAAKLSMEQWVHFMAQHPGIKNAFEFEIMKLMSEVMAHTITLHLLDAP
                     GRVRRFMRKHAELAGRLPKKELASYLNLSPETLSRLKRRGKI"
     gene            complement(2354..3859)
                     /locus_tag="VGA59_16620"
     CDS             complement(2354..3859)
                     /locus_tag="VGA59_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX9721343.1"
                     /translation="MEELNALFHGFSVALTPFNLMLMFIGIILGVIIGVLPGLGGANG
                     VAILLPLTFSMPPTSAIIMLSCIYWGALFGGAITSILFNIPGEPWSVATTFDGYPMAQ
                     QGRAGEALTGAFTSSFVGALVAVLMFTFLAPLIANFALQFGPPEFFAVYLLTFCSFVG
                     TGKGNPFKIIASMMLGFAMAAVGIDIVTGQLRLTFGFTELLSGFHFLVAVIGLFGIGE
                     ILLSMEEGLSFTGHTAKINPRIVFQTWAKLPRYWATSIRSCLIGCWMGIIPGGATPAS
                     FMSYGIARQMSRDGGKFGTGQLEGVVAPETAAHAAGTSALLPMITLGIPGSPTAAVLL
                     GGLMIWGLQPGPLLFVEQKDFVWGLIASMYLGNIAGLIVVLTMVPLFAAILRVPFSII
                     APIIVVICAIGAFTVKNNMLDVWLMVVFGVVGYVFKKLDYPLAPLVLALVLGDAAESS
                     FRQAMLLSQGSITVFWSNWLVGGLTTLALVLLAWPLYTALRERLRAAEQSG"
     gene            complement(3887..4393)
                     /locus_tag="VGA59_16625"
     CDS             complement(3887..4393)
                     /locus_tag="VGA59_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX9721344.1"
                     /translation="MNNSKEAAQRAVRIGTMDAIVAALIFTLGALVVYESYRLGSGWD
                     ENGPQAGYFPFYIGLLVCVSSAVVFVQSLLRFKSDMRVFVRQDQFGQVLVILLPSAGY
                     VLGIQLIGIYVPSAVFIGLFMKIVGKYSWLRSTVICLAVSVTAFLLFEVWFKIPLPKG
                     PFETLIGY"
     gene            complement(4503..>4728)
                     /locus_tag="VGA59_16630"
     CDS             complement(4503..>4728)
                     /locus_tag="VGA59_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9721345.1"
                     /translation="LRGIFMPAGVSQDVVNYYIEFFKKVRGTAEWKKLMEDGAFNQSF
                     MTGKEYVKWVANAEKVHRDLMKDAGFLAKK"
BASE COUNT          926 a         1499 c         1495 g          808 t
ORIGIN      
        1 ggcgctgtcg ccctcgatcg cgatcaccgg ctggccgctg accacggcgg ccgcgacgga
       61 aaagcccatg ccgatgccca tcacgcccca ggtgccgacg tccaggcgct tgcgcggctt
      121 gtacatgtcg acgatgctgc gggtgaagtc gagcgcgttg gcgccttcgt tgaccaggat
      181 ggcgtccggg ttggccttga cgatgtcgcg caccacgttc agcgcgctgt ggaaattcat
      241 cggcttcgga tctttgacca gggtctcggc cattctggcg atgttcttgt tcttgcgctc
      301 ggcgatggcg ccgagccatt ccgccgacgg cctggaccag ttgaagccga tgccggcgag
      361 cagcgcggcc acgcacgaac cgatgtcgcc gaccaccggg gcgtcgatgc ggacgttgct
      421 gtccgcctcc tgcggcgaaa tgtccacctg gatgaatctc tggccgcccc aggccttgtg
      481 gtccttgccg ccccaggtct tgcccttgcc gtgcgagagc agccagttga ggcgcgcgcc
      541 gatcagcatc accacgtcgg cctcgggcag cacataggag cgggcggcag cagcggaatg
      601 ctcgtgcgtg tcgggcagca ggcccttggc catggacatg ggcaggtagg ggatgccggt
      661 cttctcgatg agcgcctgga tgtccgcgtc ggcctgggca taggcggccc ccttgcccag
      721 cacgatgagc ggacgcctgg cgctcttgag cagatcgagc gcgcgcttca cggcgtccgg
      781 ggccgggatc tggcgcggcg ccggatcgac caccttgatc agcgatttcc tgccggcttc
      841 cgcgtccatg gtctgggcga agatcttccc gggcaggtcg aggtagaccc cgcccgggcg
      901 gccggagacc gcggcgcgga tcgcgcgcgc gatgcccaca ccgatgtcct cgacgtgcag
      961 cacgcggaat gcggccttgg cgagcggctt ggcgatggcg agctgatcca tctcctcgta
     1021 gtcaccctgc tggaggtcga cgatctcgcg ctcgctggag ccgctgatga ggatcatcgg
     1081 gaagcaattg gtggtggcgt tggccagtgc ggtcaggccg ttgaggaaac cgggggcgga
     1141 caccgtcagg cagatgcccg gcttttgcgt catgaagccg gcgatggcgg cggcgttgcc
     1201 ggcgttctgc tcatggcgga acgatatcac gcgcatgccc gcggcctgtg ccaggcgggt
     1261 gaggtcggtg atcggaatgc cgggcagacc gaagatcgtg tcgatgccgt tcaacttgag
     1321 cgcatcgatg accaggtgaa agccgtcggc cactgcttgt gatcgtgcat cggttgtcat
     1381 gctgagatcc gtcatggtga gatgcctcgc gttctcgccg tcagttgaac aagtgttttt
     1441 ccaatggata tctgccgtgg aaggaaaaca gggcggaatc ttctcaattc acggcagatc
     1501 caggccacct aaacgtgcct gtgattcaga gtgcctaaca tgatgaacca cgtgtgcggg
     1561 tcatcgtgat aggcgctctt actcgcacat atcatagggg gcagcatgca catgcactct
     1621 tgaccgcgat caagattcgc ctttcccctg cgcgttctgt gggttagagc ggaattcgct
     1681 atactcctcg ctcgcggtag acagccccgc gaccgccatc atgcccctgc tcagccaaca
     1741 ccccgaagcc aggatcgtcc gcgttcaact gcgccagaac ctgatcctga agcacctcga
     1801 cgcgggccag tgggaacaga tcgagacgtt gctggaggtc atcgactatg caaagaacga
     1861 ggcccttgaa ctccaggggg cgctgtcgat ggagcagtac ttcatcatcg acggcatcct
     1921 caagcgcgtg gtgtccaacg cccagggcaa ggaaatgatc ctgcggttcg ccgaggaaag
     1981 cgacatcgat acgagttacg cggcctggcg cctgaaacgg ccggtcccct actcgattcg
     2041 cgccgtcacc aaggtgcgcg ccgcgaagct ctccatggaa cagtgggtgc atttcatggc
     2101 gcagcatccc ggaatcaaga acgcattcga attcgagatc atgaagttga tgagcgaggt
     2161 aatggcccac accatcaccc tgcacctgct ggatgcgccg ggaagggtgc ggcgcttcat
     2221 gcgcaagcac gccgaactcg ccgggcggct gcccaagaag gaactcgcgt cctatctcaa
     2281 tctgtcgccg gaaacgctaa gccgcctcaa gcggcggggt aaaatctgac cgtctcaaac
     2341 ggcgcggaaa gatctaacct gactgctccg ccgcgcgcag ccgttcgcgc agcgcggtgt
     2401 aaagcggcca cgccagcagc acgagcgcca gcgtggtgag gccgcccacc agccagttcg
     2461 accagaacac ggtgatgctt ccctgggaga gcaacatcgc ctgccggaac gagctttcgg
     2521 cggcgtcgcc cagcaccagg gccagcacca ggggcgcgag cggatagtcg agcttcttga
     2581 agacgtagcc gacgacgccg aacaccacca tcagccacac gtccagcatg ttgttcttca
     2641 ccgtgaacgc gccgatggcg cagatcacga cgatgatcgg tgcgatgatg gaaaacggca
     2701 cgcgcaggat ggcggcgaac agcggcacca tggtcagcac cacgatcagc cccgcgatgt
     2761 tgccgaggta catgctagcg atcaaccccc acacgaaatc cttctgctcc acgaatagca
     2821 gcgggcccgg ctgcagaccc cagatcatca gccccccgag cagcacggca gcggtaggcg
     2881 agcctgggat gcctagcgtg atcatcggca gcagcgccga ggtgccggcg gcgtgggcgg
     2941 cggtttccgg tgcgaccacg ccttcaagct ggccggtgcc gaacttccct ccatcgcgcg
     3001 acatctggcg cgcgatgccg taactcatga acgaagccgg cgtcgcgccg cccggtatga
     3061 tccccatcca gcagccgatg aggcagctgc ggatcgaggt cgcccagtaa cgcggcagct
     3121 tggcccaggt ctggaacacg atcctggggt tgatcttggc ggtgtgaccg gtgaacgaca
     3181 gcccttcctc catggaaagc aggatttcgc cgatgccgaa cagtccgatc accgccacca
     3241 ggaagtggaa cccggagagc agttccgtga agccgaacgt gagccgcagc tggccggtaa
     3301 cgatgtcgat gccaaccgcc gccatggcaa agccgagcat catcgaggcg atgatcttga
     3361 aaggattgcc cttgccggtg ccgacgaagc tgcagaaagt cagcagatac accgcgaaga
     3421 actcgggcgg cccgaactgc agcgcgaaat tggcgatgag cggcgcgagg aaggtgaaca
     3481 tgaggaccgc gaccagcgcc ccgacgaagg aggaggtgaa cgcgccggtc agcgcctcgc
     3541 ccgcacgccc ctgctgcgcc atggggtagc cgtcgaacgt ggtcgccacc gaccacggct
     3601 cgcccggaat attgaacagg atcgaagtga ttgcgccgcc gaacagcgcg ccccagtaga
     3661 tgcaggagag catgatgatc gccgaggtcg gcggcatgga gaaggtgagc ggcagcagga
     3721 tcgcgacgcc gttcgcgccg cccagtcccg gcagcacacc gatgatgacg ccgaggatga
     3781 tgccgatgaa catcagcatc aggttgaacg gcgtcagcgc gaccgagaag ccgtgaaaca
     3841 gcgcgttgag ttcttccacc ttcgatggcc ctcgcccgtg cttgcgctaa tacccgatca
     3901 gcgtctcgaa tggccccttg ggcaggggga tcttgaacca cacctcgaac agcaggaacg
     3961 cggtcacgct taccgcgagg cagatcaccg tgctgcgcag ccagctgtac ttgccgacga
     4021 tcttcatgaa cagcccgatg aacaccgccg aaggaacata gatgccgatc agctggatcc
     4081 ccagcacgta gcccgcggag ggcagcagga tcaccagcac ctgcccgaac tgatcttgcc
     4141 ggacgaaaac cctcatgtct gacttgaacc tgagcaggct ttgcacgaac accactgcgc
     4201 tcgagacgca taccagcagt ccgatgtaga aagggaaata ccccgcctgc gggccgtttt
     4261 cgtcccagcc cgagccgagc cggtagcttt cgtagaccac cagcgcgccg agcgtgaaga
     4321 tcagcgccgc gacgatggcg tccatggtgc cgatgcgaac cgcgcgctgc gccgcttcct
     4381 tgctgttgtt catctcagcc acggtttgtt ccggtaaacg cgacaatcac cggacgcgga
     4441 ccgacatccc gcttcgaaga agaaaatggg ggcgggcgtg atgcccgtcc ccgcaggtac
     4501 cgttatttct tggcgaggaa gcccgcgtcc ttcatcaggt cgcggtgtac cttctccgca
     4561 ttcgcgaccc acttgacgta ctccttcccg gtcatgaagc tctggttgaa agcgccgtct
     4621 tccatgagct tcttccattc cgcggtgccg cgcactttct tgaagaactc gatgtagtaa
     4681 ttgaccacgt cctgcgacac gccggcgggc atgaagatgc cgcgcagc
//
LOCUS       DASWIY010000463         2037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_692945, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000463 DASWIY010000000
VERSION     DASWIY010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692945"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VGA59_16635"
     CDS             <1..545
                     /locus_tag="VGA59_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9721346.1"
                     /translation="FIQLMCQLGHPIVYLQNTTGYMVGKESEQGGMIKHGSKMIQAVT
                     NATVPQLTIMCGASFGAGNYGMCGRGYAPRFLFSWPSAKTAVMGGEQAARTMQIVTEA
                     GLARKGIAPDPEQSKAQFDKIVAMFEAQADAFYTSGLLLDDGVIDPRETRAVLAFCLE
                     TCAEAEEKQLRRMQFGVARM"
     gene            664..1842
                     /locus_tag="VGA59_16640"
     CDS             664..1842
                     /locus_tag="VGA59_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9721347.1"
                     /translation="MNYTHEHLEIQKTLKRFIDEEVNPHVDEWEAAEMFPAHQVFKRL
                     GELGLLGLTKPEEYGGAGLDYSYAMAMAEALGHTHCGGVPMAIGVQTDMATPALARFG
                     SDELRRQFLAPAIAGEMVTCIGVSEPGAGSDVAGIKSRAVKDGDDYLITGQKMWITSS
                     LQADWMCMLVNTGEGPIHKNKSLVIVPMRDGPNGKLTKGIEIAQKIRKIGMNSSDTGL
                     IYFDEVRVPQRYRIGSEGQGFIYQMQQFQEERLWAAASSLQSLTNCIEWTVEWAQQRK
                     MFGATLADQQWVQFKLAELKTEVECLRALTYRAGELYVQGQDVLELASMAKLKAGRLN
                     RIVPDACLQFWGGMGYTWENKVSRAFRDGRLASIGGGADEVMLGIVAKIMGTAKRMPV
                     "
     gene            1883..>2037
                     /locus_tag="VGA59_16645"
     CDS             1883..>2037
                     /locus_tag="VGA59_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9721348.1"
                     /translation="MRRLLIANRGEISRRIIRTAHRMGIETVAVYSDADARALHVRET
                     TAAFALGG"
BASE COUNT          407 a          630 c          679 g          321 t
ORIGIN      
        1 acttcatcca gctgatgtgc cagctgggcc accccatcgt ctacctgcag aacaccaccg
       61 gctacatggt gggcaaggag agcgagcagg gcggcatgat caagcacggc agcaagatga
      121 tccaggccgt caccaatgcc accgtgccgc agctcacgat catgtgcggc gcctctttcg
      181 gcgcgggcaa ctacggcatg tgcgggcgcg gctacgcgcc gcgctttctc ttcagctggc
      241 ccagcgccaa gacggccgtg atggggggcg agcaggccgc gcgcaccatg cagatcgtca
      301 ccgaagcggg attggcccgc aagggcattg cgcccgatcc cgagcagtcc aaagcgcagt
      361 tcgacaagat cgtcgccatg ttcgaagcgc aggccgatgc gttctatacg tcggggttgc
      421 tgctcgatga tggtgtgatc gacccgaggg agactcgcgc cgtgttggcg ttctgtttgg
      481 agacctgcgc cgaggcggaa gagaagcaac tgcggcgcat gcagtttgga gtagcgcgaa
      541 tgtgaaaggg gctgtcattg cggccccgga tcaagtccgg ggttccagca cccgcaatcc
      601 atgttggcgc cacgatggat gccggatcag gtccggcatg acagccgcag gcaggagaca
      661 agcatgaact acacccacga acacctggaa atccagaaaa ccctcaagcg cttcatcgac
      721 gaggaggtca atccccacgt cgacgaatgg gaagccgccg agatgtttcc cgcgcaccag
      781 gtattcaagc gcctgggcga gctcggcttg ctgggcctga ccaagcctga ggaatacggc
      841 ggtgcggggc tcgactattc ctatgcgatg gccatggccg aggcgctggg ccatacccac
      901 tgcggcggcg tgcccatggc gattggcgtg cagacggaca tggccacccc cgcgctggcg
      961 cgcttcggca gcgacgaact ccggcgccaa ttcctggcac cggcgatcgc gggcgagatg
     1021 gtcacatgca tcggcgtgag cgaacccggg gccggcagcg acgtcgcggg catcaagagc
     1081 cgcgcggtga aagacggcga cgattacctc atcaccggcc agaagatgtg gatcaccagc
     1141 agcctgcagg ccgactggat gtgcatgctg gtcaacaccg gcgaaggacc catccacaag
     1201 aacaagtcgc tggtgatcgt gccgatgcgc gacgggccca acggcaagct caccaagggc
     1261 atcgaaatcg cccagaagat ccgcaagatc ggcatgaact cgtccgacac gggcctgatc
     1321 tatttcgacg aagtgcgcgt tccgcagcgc tatcgcatcg gcagcgaagg ccagggcttc
     1381 atctaccaga tgcagcagtt ccaggaagag cgcctctggg ccgccgccag ctcgctgcag
     1441 tcgctcacca actgcatcga atggaccgtc gagtgggcgc agcagcgcaa gatgttcggc
     1501 gccaccctgg cggaccagca atgggtgcag ttcaagctgg ccgagctcaa gaccgaagtc
     1561 gagtgcctgc gcgccctgac ttatcgcgcc ggcgagctgt atgtgcaggg ccaggacgtg
     1621 ctggagctcg cttctatggc caagctcaag gcggggcgac tgaaccgcat cgtgccggat
     1681 gcctgcctgc agttctgggg cggcatgggc tacacctggg agaacaaggt ttcgcgcgcg
     1741 ttccgcgatg gacggctggc gtccatcggc ggcggcgccg acgaggtgat gctgggcatc
     1801 gtggccaaga tcatgggcac ggcgaagagg atgccggtgt gatgctcgcg cagcgtgcag
     1861 ggagatgagc cgcgtgaagc ctatccggag attgctcatc gcaaaccggg gagagatttc
     1921 ccgccggatc attcgcaccg cccaccgcat gggaatcgag acggtggcgg tctattccga
     1981 cgccgacgcc cgagccctgc acgtgcgcga aaccactgcg gctttcgctc tgggcgg
//
LOCUS       DASWIY010000464         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2205525, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000464 DASWIY010000000
VERSION     DASWIY010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2205525"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(399..2309)
                     /gene="htpG"
                     /locus_tag="VGA59_16650"
     CDS             complement(399..2309)
                     /gene="htpG"
                     /locus_tag="VGA59_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX9721349.1"
                     /translation="MSQKQTLSFQAEVAQLLHLVTHSLYSNKEIFLREMISNASDACD
                     KLRFEALDNNALYEDAPNLEVRVDFDKQAGTLTISDNGIGLAQQEAIDNLGTIAKSGT
                     REFVSRLSGDQKADAQLIGQFGVGFYSGFIVADKITVESRRAGLKPEEGVRWTSAGAG
                     DFEVETIERPQRGTSVTLHMREDAREYLATWKLKAIINKYSDHISLPILMRKEEWKEG
                     DENKGGEMVLTDEWERVNQASALWTRPKKDISSEQYQEFYKQISHDHEPPLAWAHNRV
                     EGATEYIQLLYIPSHASFDLWNREKVAGVKLYVKRVFIMDEAEALLPVYLRFVKGVID
                     SSDLPLNVSRELLQESRDVKAIREGCTKRVLAMLEDLAKAEKDEGASDEEKGKYAKFY
                     AEFGAVLKEGLGEDFANRDRLARLLRFASTSSDTQSVSLADYKSRMKEGQEAIYYITA
                     DTLAAAKNSPQLEVFRKKGIEVLLMTDRVDEWALNYLHEFDNTPMQSVAKGAVDLGKL
                     QDEAEKKAAEEAAEAFKPVLARLKEALKDKAQDVRITTRLVDSPACLVVTDSGMSMQL
                     ARMLKQAGQKIPEVKPVLEVNAEHPLVKKLEGSEHFDDLAHILFDQALLAEGGLPDDP
                     AAYVKRVNALLV"
BASE COUNT          428 a          862 c          823 g          550 t
ORIGIN      
        1 cgaggtggtg aagaagatcg ggaaggtcga gtgacggtcc tgggagtcat gccatgtcgc
       61 ggtctgtcat gccgggcttg acccggcatc catgaattcg ggggcatgga ttgcggctcg
      121 aggccgcaat gacagaagcg ctgtcatccc ggggcttgag ccgggaaacc tggattgcgg
      181 gtcgagcccg caatgacagg gcgtagtggg cgttgtcacc ccgggcttga cccgggaaac
      241 ttggattgcg ggtcgagccc gcaatgacaa agaaaacacc gtcatcccgg gctcgacccg
      301 ggatccatgc ccgcgtcgaa cagcctggat tgcgggtcga gcccgcaatg acaagccagg
      361 ggccaaaccc acgcccggag cccgcaatga caagctggct acaccagcag cgcgttcacg
      421 cgcttcacgt aagcggccgg gtcgtcgggc aaaccgccct cggccagcaa ggcctggtcg
      481 aacaggatat gcgccaggtc gtcgaagtgc tcggagccct ccagcttttt caccagcggg
      541 tgttcggcat tgacttccag caccggcttg acctccggga tcttctgccc cgcctgcttg
      601 agcatccgcg ccagctgcat gctcatgccg ctgtcggtga cgaccaggca ggccggtgaa
      661 tcgaccaggc gcgtggtgat ccgcacgtcc tgcgccttgt ccttgagcgc ctccttcagc
      721 ctggccagca ccggcttgaa cgcctcggcc gcttcctcgg cggccttctt ttcggcctcg
      781 tcctgcagct tgcccaggtc caccgcgccc ttggccaccg attgcatggg cgtgttgtcg
      841 aactcgtgca ggtagttcag cgcccactcg tcgacccggt ccgtcatcag cagcacctcg
      901 atgcccttct tgcggaacac ctcgagctgc gggctgttct tcgcggctgc cagcgtatcg
      961 gcggtgatgt agtagatcgc ctcctggcct tccttcatgc gtgacttgta atcggccagc
     1021 gacacgctct gcgtgtcgga actggtggag gcgaaccgca gcagtcgcgc caggcggtcg
     1081 cggttggcga agtcctcgcc caggccttcc ttgagcaccg cgccgaattc cgcgtagaac
     1141 ttcgcgtact tgcccttttc ctcatcggat gcgccctcgt ccttttccgc cttggccaga
     1201 tcttccagca tggccagcac acgcttggtg cagccctcgc ggatcgcctt cacatcgcgg
     1261 ctttcctgca gcagctcccg gctgacgttg agcggcaggt ccgacgaatc gatcacgccc
     1321 ttgacgaaac gcaggtacac cggcagcagg gcctcggctt cgtccatgat gaagacgcgc
     1381 ttcacgtaca gcttgacccc ggcaaccttc tcgcgattcc acaggtcgaa cgacgcatgg
     1441 gaggggatgt agagcagctg gatgtactcg gtcgcgcctt cgacgcggtt gtgcgcccag
     1501 gccagcggcg gctcgtgatc gtgactgatc tgcttgtaga actcctggta ctgctcggac
     1561 gagatgtcct tcttcggccg ggtccacagc gcgctcgcct ggttgacgcg ctcccattcg
     1621 tcggtgagca ccatctcgcc gcccttgttc tcgtcgcctt ccttccattc ctccttgcgc
     1681 atcaggatgg gcagggagat gtggtcggag tacttgttga tgatcgcctt gagcttccag
     1741 gtggccaggt attcgcgggc gtcctcgcgc atgtgcaagg tgacgctggt gccgcgctgc
     1801 ggccgctcga tggtttccac ctcgaagtcg ccggcccccg cgctggtcca gcgcacgccc
     1861 tcttcgggct tcagcccggc ccggcgcgac tccaccgtga tcttgtccgc gacgatgaaa
     1921 cccgaataga acccgacgcc gaactggccg atcagctggg cgtccgcctt ctggtcgccc
     1981 gagaggcggc tgacgaattc ccgggtgccg ctcttggcga tggtgcccag gttgtcgatc
     2041 gcttcctgct gcgcgaggcc gatgccgttg tcgctgatgg tcagcgtccc ggcttgcttg
     2101 tcgaaatcca ccctcacttc gaggttgggc gcgtcttcgt acagcgcatt gttgtccagg
     2161 gcctcgaagc gcagcttgtc gcaggcgtcc gatgcgttgg agatcatctc gcgcaggaag
     2221 atctccttgt tcgagtacag cgagtgcgtg accagatgca gcagctgggc cacttcggcc
     2281 tggaatgaaa gggtctgttt ttggctcatg gcggtgaaag gaaaggcatt gcgcaaaatg
     2341 cttccgggac cttggggcgc cggccgcttt tttcaagacc gcttggtcat cagggaaatg
     2401 aaccgtgccg tgccggaact cgaattgccg tgaaaaagtc gacgcggccc tgactttgat
     2461 tgccgagccg ccccagcacg cgggacaaat ggttacatta cgtctcgatg actcgccgct
     2521 tcaggtcaat ggctgaatgc ggaccgcctg tttggctgga cgatgacggg acaccttctt
     2581 cactcatgaa tatcgacctg ctgcccttcg cctccttcgc gctggagtcc tccggcgtgg
     2641 cggaccgccc ctgcggcgaa ccg
//
LOCUS       DASWIY010000465         3148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3625929, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000465 DASWIY010000000
VERSION     DASWIY010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3625929"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VGA59_16655"
     CDS             <1..304
                     /locus_tag="VGA59_16655"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9721350.1"
                     /translation="AEYGIDPSPVSPTDSRGYRIVAGSIRALAPDALVAPYMVRGGTD
                     ARYFYELSPNVYRFLAVRIDADTMRQVHGIDEHVAIDDYLMAVRYYYNVMGQAAEP"
     gene            complement(494..2788)
                     /locus_tag="VGA59_16660"
     CDS             complement(494..2788)
                     /locus_tag="VGA59_16660"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX9721351.1"
                     /translation="MRIGGEQIDEAIIARMQGMVDVQPGLSRTALSRRLCEEFKWKSP
                     NGKLKEVSCRTALVKLHLAGALRLPEAQPFGVRLRVRRAVRETPPEPLVRQPLKAVQP
                     IELVLIGSADSAASRVWNELMDRHHYLGSGPLCGAQVRYLIRSERHGWLGGLAFSAAA
                     WRVGARDQWIGWTDAVRQQHLHRVVCNSRFVLVPTVQVPHLASHVLGLALQRLGEDFR
                     HRYGYAPELVETFIDRERFAGTCYRAANFIEVGHTQGRGRQDRAHRGGASVKRVFVYP
                     LHAQARARLCAGTVRARARVVPPKPRDWAEEELGAARLPDRRLNRRLLAIARDFYARP
                     QAQIPQACQSRAKTKAAYRFFRHPETTMDVLLEPHRQATMRRMAEHRVVLCPQDTTSL
                     NYSTHPATQELGPIGSVPEGVIGLLVHDTMAFTVEGTPLGLLDVQWWARDPQQYGQKH
                     ARKQRPIEAKESAKWLKSFERVAAAQQHCPDTLLVSVGDREADVYELFQRAQAEPRGP
                     KLLVRAEQDRLLAEDQGHLWSAVQAQPVAGMQMLHVPRRAARPARTAELAVRFAEVTL
                     KPPQRKAQLGTVQLWAVLAEEANAPAGVAPLRWLLLTSLPVRSFAAAVEKLAWYTQRW
                     GIEIFHKTLKSGCKVEERQLGSADSIQACLAIDMVVAWRVYHLAKLGRETPDVPCTVF
                     FEDAEWKALLTFVTEQPVVDGPPPSLRDAMRMVAGLGGFLGRTSDGEPGTKSLWLGLQ
                     RLDDMTVAWKIFVPHLRGPPVSST"
     gene            complement(2878..>3148)
                     /locus_tag="VGA59_16665"
     CDS             complement(2878..>3148)
                     /locus_tag="VGA59_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721352.1"
                     /translation="EEIYTPLPGPRNAERLGTFAQVDFRISREFAVRRGQVTAFFEVT
                     NATNRDNPCCIDYDIDEDHSGEAFLDRTVDYWLPLLPAVGLHWAF"
BASE COUNT          550 a         1147 c          929 g          522 t
ORIGIN      
        1 cgccgagtac ggcatcgacc cctcgccggt gtcacccacc gattcgcgcg gttaccggat
       61 cgtggccggc agcatccggg cgttggcgcc cgacgcgctg gtggccccgt acatggtgcg
      121 tggcggcacc gacgcgcgtt acttctacga gctttcgccg aacgtctacc ggttcctggc
      181 cgtgcgcatc gacgcggaca ccatgcgcca ggtgcacggt atcgacgagc acgtcgccat
      241 cgacgactac ctgatggccg tccgctacta ctacaacgtc atgggccagg ccgcggaacc
      301 ctagcaaaga ggccggcccc agcggccgct gccggctccg accatcacgg taacggcgca
      361 gttcgtgtgc atccggagct tggcgcgtcg gtttgcgcga ctcgctgcgc tcgcatcccc
      421 ccttaccccc ttctgaagaa ggggggtaag ttacgcttgt accccttctg aagaacttat
      481 cgttacccac aacttaagtg ctggacacgg gcggtccgcg cagatgcggg acaaatattt
      541 tccaggcgac ggtcatatcg tccaggcgct gcaggccgag ccacaaggat ttcgtgcccg
      601 gctccccatc gctggtgcga ccgagaaagc cgccgagacc tgccaccatc cgcatcgcat
      661 cacgcagact cgggggtggg ccatcgacga ccggctgttc ggtaacgaag gtcagcagtg
      721 ctttccactc ggcatcttcg aaaaacaccg tgcacggcac gtccggcgtc tcacgcccca
      781 gcttcgccag gtgatacacg cgccaggcca ccaccatatc gatcgccagg cacgcctgaa
      841 tggaatcggc gctgccgagc tgccgttcct ccaccttgca gccgctcttg agcgtcttgt
      901 gaaagatctc gatgccccag cgctgggtgt accaggcgag cttctcgacg gccgccgcga
      961 aactgcgtac cggcaagctg gtcagcaaca gccagcgcaa gggcgccacc cctgcggggg
     1021 cgttcgcttc ctccgccaac accgcccaga gctgcacggt gccgagttgg gctttacgct
     1081 gcggcggctt gagcgttact tcggcgaagc gcacggcgag ctctgccgtg cgcgcgggcc
     1141 tggcggcgcg ccgcggcacg tgcagcattt gcatgccggc caccggctgg gcctgcaccg
     1201 ccgaccacag gtgaccctga tcctcggcca acagccgatc ctgctcagcc cgtaccaaca
     1261 gcttcggccc cctcggttca gcctgtgccc gctgaaacag ctcgtacacg tccgcttccc
     1321 gatcaccgac gctgaccagc agcgtgtccg ggcagtgctg ctgcgctgcg gcgacccgct
     1381 cgaagctctt cagccacttg gcgctttcct tcgcctcgat cggccgctgc ttgcgcgcgt
     1441 gcttctgacc gtactgctgc gggtcccgcg cccaccactg cacgtcaagc agacccagcg
     1501 gcgtgccctc gaccgtgaac gccatcgtgt cgtgtaccag caaaccgata acgccctccg
     1561 gcaccgaacc gatcggaccg agttcctggg tcgccggatg cgtgctgtag ttcaagctcg
     1621 tcgtgtcttg cgggcacagc acgacccgat gctcggccat ccgccgcatc gtggcttgcc
     1681 ggtggggctc gagcaacacg tccatcgtcg tctcaggatg ccggaaaaat cgatacgctg
     1741 ccttggtctt cgcgcggctc tgacaggctt gcgggatctg cgcctgcggg cgggcgtaaa
     1801 aatcccgcgc aatcgccaac agccgccgat tcaaccgccg gtccggtaaa cgcgcagcgc
     1861 ccagttcctc ctccgcccaa tcccgtggct tgggcggtac cacccgcgct ctggcacgga
     1921 cggtcccggc acacagccgc gcgcgcgcct gcgcgtgcag cggatacacg aacacccgct
     1981 tgacgctggc accgccacgg tgtgcgcgat cctgccgacc ccgcccttgc gtgtggccca
     2041 cctcgatgaa gttcgccgct cgatagcagg ttcccgcaaa gcgctcacga tcgatgaacg
     2101 tctccaccag ctccggcgca tagccgtagc ggtgacgaaa atcttcgccc aaccgctgca
     2161 gcgccaaccc cagcacgtgc gacgccaaat gcggcacctg cacggtcggc acgagcacaa
     2221 accgactatt gcacaccacc cgatgcaagt gctgctgccg caccgcatcg gtccacccaa
     2281 tccattgatc ccgcgcgccg acccgccagg cggccgccga gaaggccagc ccgcccaacc
     2341 agccgtgccg ctcgctgcgg atcagataac gcacctgcgc cccgcataac ggacccgacc
     2401 ccaggtagtg atgccgatcc atcagctcgt tccagacccg cgacgcggcg ctatccgcac
     2461 tgccgatcag caccaactcg atcggctgaa cggccttcag cggctgacga accagcggct
     2521 ccggcggggt ctcccgaacc gctcggcgca cccggagtcg caccccaaac ggctgcgcct
     2581 caggcaaccg cagcgcgccc gccaggtgca acttcacgag cgccgtccgg cagctgactt
     2641 ccttcagctt gccgttcggc gacttccact tgaactcctc acagaggcgc cgtgacaacg
     2701 ccgtgcgcga caagccgggt tgcacatcga ccatgccttg catccgcgcg atgatcgcct
     2761 catcgatttg ctcgccgcca atcctcatga cggggcaatc tacaagagta cgcctcccgt
     2821 gtccagcaaa aagatgtggg acacgctaag ttctgaagaa gggggggagt tgccccttca
     2881 gaatgcccag tgcagcccga ccgccggcag cagcggcagc caatagtcca ccgtgcggtc
     2941 caggaatgcc tcgcccgagt ggtcttcatc gatgtcgtag tcgatgcagc acgggttgtc
     3001 gcggttggtc gcgttggtca cttcgaagaa cgcggtcacc tggccgcgcc ggaccgcgaa
     3061 ctcgcggctg atccgaaaat cgacctgggc gaaagtgccg aggcgttccg cgttgcgcgg
     3121 ccccggcagc ggggtgtaaa tctcttcg
//
LOCUS       DASWIY010000466         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_322242, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000466 DASWIY010000000
VERSION     DASWIY010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322242"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(519..1415)
                     /locus_tag="VGA59_16670"
     CDS             complement(519..1415)
                     /locus_tag="VGA59_16670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9721353.1"
                     /translation="MKHELNVVIGIATAGRREQMPLTLGQIARQRRLPQRIVICPAAA
                     DDYDDSASALVACPVEVVRGPRGLPAQRNAILAACGAADVVVFMDDDFYPAPDYVEQV
                     ISLLVTHPGIVVATNRPVADGATGPGIAHDEAVRIVESLPAVLQSTPHIKDTYGGYGC
                     NMAVRLAPVRRHGLRFDENLPLYGWLEDIDFSRCMAAHGRIVKCSSLRGVHLGTKRGR
                     TSGLRLGYSQVANPVYMLRKGTLSFRYAARQLGRNVVKNATRALWPEPWVDRRGRLKG
                     NWLAMLDLLRGRIAPNRIHTLK"
     gene            complement(1412..>2411)
                     /locus_tag="VGA59_16675"
     CDS             complement(1412..>2411)
                     /locus_tag="VGA59_16675"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX9721354.1"
                     /translation="HMMGRLALVLAGVAIGVGGTLLSPLLRETPALGILATANGIVAA
                     FNLGWYFQGTLRFRTSVMIEVAGFAISLPLILLFVRGPGDAWIVLAALLASGVVCTAV
                     AHGIALRSMDRHGIRWKGGFSLVRESTALFLHKGLTMMMASSSTYLLSLFATAAQVGW
                     YGVAERLAAVGLSLMQPANHVLVGTVARRISAKESEAEAYTLMRSSLIVMTSFGMLLL
                     AGAALLAGAVVPFVMGPSFAPTVPMLQILALMFPFAAFCQVTSGYILIPLRFDRLVSA
                     VSLFGAAVTVGLVVALGLGLGGIGVAWARTIGYVAIALFLLAILHRKQLLTRIWQA"
BASE COUNT          446 a          797 c          820 g          348 t
ORIGIN      
        1 gcgcgggacg ttgaaattga tcagcagcac tgctcatggg cggactccag caaaccgggg
       61 ccgagcgtgc gatgaacttc gatggcgcaa ccgatgatgc gatgcgtcaa tggacgcgtt
      121 gtcttccagg ttgcttagtt gtcttctccg tgttctctgt gcctctgtgt cctccgtgtt
      181 caaagaaacc ccgcgagtac gcctgcatgg caacagacgg gcgctgtcgg gaggccgcgt
      241 ctgacacaga ggacacagag gcacagagaa gcgcagagga ttttcgcccg cacgagtgga
      301 tcccggcccc cgtgtcgggt atggggcggc gcttctgcgg gatgacggcc gatgagagcg
      361 aacagtcttt aatgtcttgc aactggcgaa gatcgacaaa gttagcgcat atgggcgaaa
      421 gccggtatcc actcgtggcc ggggagccga cttcccgcag tggatcccgg ccttcgccgg
      481 gatgactgcg ttcggggcgg ggcaccggga ggatggtttc atttcagtgt gtggatgcgg
      541 ttgggcgcga tgcggccgcg cagcaggtcg agcatggcca gccaattgcc cttcaagcgc
      601 ccgcggcgat cgacccaggg ttcgggccac agcgcacgcg tcgcgttctt caccacgttg
      661 cggcccaact ggcgcgcggc gtagcggaac gacagcgtgc ccttgcgcag catgtacacg
      721 gggttggcca cctgcgaata gcccagccgc agcccggacg tccggccgcg cttggtgccc
      781 aggtgcacgc cgcgcagcga cgagcacttg acgatgcgcc cgtgcgcggc catgcagcgg
      841 ctgaagtcga tgtcctccag ccagccgtac agcggcaggt tctcgtcgaa gcgcaggcca
      901 tgccgccgca ccggcgccag ccgcacggcc atgttgcagc catacccgcc gtaggtgtcc
      961 ttgatgtggg gagttgactg caatacggcc ggaagcgatt cgacgatgcg cacggcttcg
     1021 tcgtgcgcaa tgcccggccc ggtcgcaccg tcggccaccg gacgattggt cgccaccacg
     1081 atgccgggat gggtgaccaa gagcgaaatc acctgctcca cgtaatccgg cgccgggtag
     1141 aagtcgtcgt ccatgaagac gacgacatcg gccgcgccgc aggcagcgag gatcgcgttg
     1201 cgctgggcgg gcaggccgcg cggcccgcga acgacttcca ccgggcacgc aaccagcgcc
     1261 gatgcgctgt cgtcatagtc gtcggcagca gccgggcaga tgacgatgcg ctgcggcagg
     1321 cgccgctgcc gtgcgatctg ccccagcgtg agcggcatct gctcccggcg gcccgcggtc
     1381 gcgatcccga tcaccacgtt cagctcgtgc ttcatgcctg ccagatcctc gtcaacagct
     1441 gcttgcggtg caggatggcg agcaggaaca gcgcgatcgc gacgtagccg atggtgcgcg
     1501 cccacgcgac gccgatgccg cccagcccca agccgagcgc gacgaccagc ccgacggtca
     1561 ccgcggcacc gaacaggctg acagcggaca cgaggcggtc gaaacgcaag gggatgagga
     1621 tgtagcccga ggtcacctgg cagaacgcgg cgaaggggaa catcagggcg aggatctgaa
     1681 gcatcggcac ggtcggggcg aacgaaggcc ccatgacgaa cggcaccacg gcgccggcca
     1741 gcaatgcagc gcctgcgagc agcagcatgc cgaagctcgt catcacgatg agcgagctgc
     1801 gcatcagcgt gtaggcctcg gcctcgctct ccttggcgct gatgcgccgc gccaccgtgc
     1861 ccaccagcac atggttggcc ggctgcatca ggctgaggcc gacggccgcg aggcgttcgg
     1921 ccacgccgta ccagcccacc tgggccgcgg tcgcgaacag gctgagcagg taggtcgatg
     1981 aactggccat catcatcgtg aggcccttgt gcaggaacaa ggccgtcgat tcgcggacca
     2041 gcgaaaagcc gcccttccac cggatgccgt gccggtccat cgagcgcagc gcgatcccat
     2101 gggccacggc ggtgcacacc acgcccgagg ccagcaaggc ggccagcacg atccacgcat
     2161 cgcccgggcc tcgaacgaac agcaggatca gcggcaggct gatggcgaac ccagccactt
     2221 cgatcatcac cgaggtgcgg aatcgcagcg tcccctggaa gtaccagccg aggttgaacg
     2281 cggccacgat gccattggcc gtggccagga tgcccagcgc aggggtctcg cgcagcagcg
     2341 gcgacagcag cgtgccgccc acgccgatgg ccacaccggc caagaccagg gccaggcggc
     2401 ccatcatgtg g
//
LOCUS       DASWIY010000467        11893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1838989, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000467 DASWIY010000000
VERSION     DASWIY010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11893
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838989"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VGA59_16680"
     CDS             <1..655
                     /locus_tag="VGA59_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEX9721355.1"
                     /translation="TRDASAAAAPQAGSARVSEGQPPHGPEVAMLALAYGLAGFGYII
                     TATFLPVIARQSLRDSAWLDMFWPILGAGVIAGVLIASRLRGHGDQRLRLGACYLVQA
                     LGIATSLWSPTLAGFAIGSLLLGLPFTAITFFALQEVRRMRPTAPSGLMGLITAMYGL
                     GQIVGPPLAAALVARSAHPAEGFALSLEIAAGALVLGALIYFFMARIYPQAHPQGRH"
     gene            741..1637
                     /locus_tag="VGA59_16685"
     CDS             741..1637
                     /locus_tag="VGA59_16685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9721356.1"
                     /translation="MPQRMTAHDFDQELLILFDAYVHGNLDRRGFLNQAQRFAKAGVT
                     AAGLLAALSPDFAAGQQVRPDDPKLKTERLSYPSPAGSGTMKGYLARPASAGANKLPA
                     VLVIHENRGLNPHIEDIARRLALDGFMAFAPDALTPQGGYPGDEDKARGLFARLDQAK
                     TEEDFLAGARWLDKRGDASGKLSAIGFCWGGGIVHVLATRMPELDAGVAFYGNTPAPE
                     EAAKVKAALLIQQAAIDERINAAWPAYEAALKAAGVKYMAYRYPGTQHGFNNDTTPRY
                     DAAAAKLAWGRTIPFLQATLAT"
     gene            complement(1691..2140)
                     /locus_tag="VGA59_16690"
     CDS             complement(1691..2140)
                     /locus_tag="VGA59_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEX9721357.1"
                     /translation="MAFPLDAAAVTHGIQLAVAPVFLLTAVSGMIGAVAGRLARIIDR
                     ARTVEDRARTCTDDEFLQRARTELEELRLRGRLTNGCIALLTTCAFLIGLTIIVLFLG
                     ETTAFQINRIAVGSFLLGVVCFLLALLCFLVETVVATRLLNFHMLRP"
     gene            2200..2796
                     /locus_tag="VGA59_16695"
     CDS             2200..2796
                     /locus_tag="VGA59_16695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9721358.1"
                     /translation="MNTRHATPDKEQIALLEPFERLGLDRIHLVATAAQARSAHAELA
                     AARAWGFDTESKPTFVKDQVSQGPHVVQLSTAHKAWVFQLHDMECRTAVANLLSLPGV
                     VKAGFGLGDDRKRIASKLGVEPVDVLELNAVFRDRGYRKDMGVKGAVAVLFNRRFIKS
                     RKATTSNWANPRLTDAQLIYAANDAYAALRVFDALDLA"
     gene            complement(2841..3260)
                     /locus_tag="VGA59_16700"
     CDS             complement(2841..3260)
                     /locus_tag="VGA59_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721359.1"
                     /translation="MFRKILLASVIAASFASVPLTSFAQQRTITIREAPPPPREEAVP
                     DMRRGQEWAPGHWAWRNGKHAWAPGKMMRERRGQHWVADTWVERKVWVLQAGHWERGA
                     RSRDRDGDGVRNRDDRDRDGDGVRNRNDSAPNNPNRQ"
     gene            complement(3397..3945)
                     /locus_tag="VGA59_16705"
     CDS             complement(3397..3945)
                     /locus_tag="VGA59_16705"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9721360.1"
                     /translation="METGKQGGSVALYWDFENLHASLYEEAQGEGAYGSKSDNRFKVQ
                     EPLIDVQAIVDLAASFGPIAIDRAYCNWQYFGRYRDVLLQNGIELIQLFPPGGSSKNG
                     ADIRLCLDAMEHLGRFEHIATVVVVGGDSDFTPLAQSIRAAGRTLVGVGARKSTNRHW
                     ASNCHEFRYYDALAGGAAPVGG"
     gene            complement(3953..4957)
                     /locus_tag="VGA59_16710"
     CDS             complement(3953..4957)
                     /locus_tag="VGA59_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9721361.1"
                     /translation="MQYRQLGKSNLKVSALCLGTMMFGDQTGRDEAQAILADAREHGV
                     NYIDTADVYTNGASESMVGELLKGQRHDWVLATKLGNKMSERVNEGHFSRTWMLREVE
                     ASLKRLQTDHVDILYMHRDNNGMDLEEPLRALDAMLRAGKIRYWGVSNFRGWRIAEIM
                     RVAGQLGMPGPAVCQPYYNLLNRMPEVEILPACEHYGIGVTPYSPIARGVLTGKYRPG
                     QPPAEGSRAARGDQRIAETELREESLRIAQLLEAHAQGKGIALAQFATAWVLAHRAVS
                     SVIAGPRTLRQWQDYLPALDYVVTGEDEALVDSFVRPGHPSTPGYNDPAYPLHPRTRG
                     "
     gene            complement(5183..6007)
                     /locus_tag="VGA59_16715"
     CDS             complement(5183..6007)
                     /locus_tag="VGA59_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721362.1"
                     /translation="MSEPLTANKPVVSVQPSQPTVEAAQAPASPTVAGPAATADSAAG
                     NVVPIPSQPQLAAASPAPVSPQDRYYTHLRSRVRWVPTRVGSQKFSTPDLDSRLLLAK
                     SAASRAGLSALGLGFKDVYGIINAETSWIPRMGASKDGTPNLGIAQFEPATARALGMR
                     NPEDAVEAVHVAALHMKEAAIWSANRIAGLKLGADERAAKLREGISIYYNLSTRGRNT
                     WNGRNTGKLPRETQLHIQNARVGAQQAEFFDAQLRAMNFKRANESAVVIASNQVRR"
     gene            complement(6236..6910)
                     /locus_tag="VGA59_16720"
     CDS             complement(6236..6910)
                     /locus_tag="VGA59_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9721363.1"
                     /translation="MLSDKTPDAELAALATAGDPRAFEAIMRRHNRLLFRTARSILRS
                     DAETEDAVQDAYLNAWRALHTFRDDAKLSTWLVRIVLNEALKRVRRRASNVVMFDSAA
                     PLGEGRTQATMEADHDQQPDLVASRAEMRRLMEARIDELPQLFRTVFMLRAVEEMSVE
                     EVSTALDLPEATVRTRFFRARAQLRESLSRDMDFALEDAFSFDGARCDRIVASVLAGI
                     HAANPP"
     gene            complement(7000..7764)
                     /locus_tag="VGA59_16725"
     CDS             complement(7000..7764)
                     /locus_tag="VGA59_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9721364.1"
                     /translation="MKLNILSDLHLSQGELALPQNDADVVVLAGDVARPPEAIAWAAR
                     LGKPVLYVPGNHEFYGGSLRSSVAQLKQLCEGTSIHVLDDETQLIGNVRFLGSTLWTD
                     FMLFGEGQGRQAAMDQAVRLMRDFSRIRVDDASQALFSPLDSVSLFQRHAAWLANELA
                     QPHEGPTVVITHHAPSRASIHPRFEGSLVNACFVSDADYLLDGDRAQLWIHGHTHDSF
                     DYVVKGTRVVCNPRGYARGGVNENAAFDPDFMVRVS"
     gene            7839..8363
                     /locus_tag="VGA59_16730"
     CDS             7839..8363
                     /locus_tag="VGA59_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9721365.1"
                     /translation="MTLSSSFQADGALELLTDLHARTLHQCASLRRLAHDLAERGCDD
                     QARSAAETLLRYFDVIPPRYYSDEEEDLFPALLESMAGSDAVCLRDLTAGMARQHRAL
                     ETMWERLRKALAAVANGQHTLLDSAEVEAFAALNESHIEREQAELLPMAQRLLTDDAL
                     AQIGASMRERHRVD"
     gene            complement(8360..8575)
                     /locus_tag="VGA59_16735"
     CDS             complement(8360..8575)
                     /locus_tag="VGA59_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721366.1"
                     /translation="MDQPSPVIAGLTGLTGLTRNPWRQEARRACRDGSRVEPGMTAVG
                     KNRRLLALMHEIDHNGLKPLLDPLRKR"
     gene            complement(8583..8948)
                     /locus_tag="VGA59_16740"
     CDS             complement(8583..8948)
                     /locus_tag="VGA59_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HEX9721367.1"
                     /translation="MKSKAGTGGLVYSTDAGRMCPACRRPVAQCVCAQARQPAAPSDG
                     IVRVSRETKGRGGKAVTVVKGLPLDATLLGKVGRQLKAACGSGGTVKDGVIEVQGDHC
                     VRVVEMLKAQGHTVKRAGG"
     gene            complement(9024..9671)
                     /locus_tag="VGA59_16745"
     CDS             complement(9024..9671)
                     /locus_tag="VGA59_16745"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX9721368.1"
                     /translation="MLTLHGSPISNYYNKVKLALLEKGVPFAEAYAATHSTDEQILGA
                     SPLGKIPFITTGQGALCESQPLLEWIEDSWPQPPLLPAEAFAAAKVRELTTFIDWHLE
                     IVARQLYGPAFFGAAPLSEANAARIRKQLVAHIAAFKRLAKFAPYVAGDSFTQADCSA
                     FNHLPLVALATKLVFGEDLLAAGGIDHKAYTRLIAQRPSAQKVVADRKAAAQIKV"
     gene            complement(9753..10334)
                     /locus_tag="VGA59_16750"
     CDS             complement(9753..10334)
                     /locus_tag="VGA59_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEX9721369.1"
                     /translation="MPLPAPQARTHLHTRAVVYRGYHREDGLWDIEADMTDTKTYPLE
                     RSERGLMPPGTPIHGMSIRVTVDDTLTIREIASSSDHTPFDECQQGKEPMQRMVGATM
                     GPGWRQAIEKALGGVRGCTHLRELLFNMATAAYQTIPSYQHRLRRLAGVPEPDGDKPP
                     YHLGKCIAWDFDGPVVQRHYPRFAGWQPLKRRG"
     gene            10439..10864
                     /locus_tag="VGA59_16755"
     CDS             10439..10864
                     /locus_tag="VGA59_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX9721370.1"
                     /translation="MPKASSKSLVAQPRPTDEKITVNLGCVDLGQIDLLVQEGFYANR
                     TDLIRTAIRNQIATHGDALRQAVSRKTLVLGIQHFSKADLQAVQAGGTRLQIRVLGLA
                     SIAPDVTPALALATIESITVLGTLQASAAVRAALTKRIR"
     gene            10888..>11893
                     /locus_tag="VGA59_16760"
     CDS             10888..>11893
                     /locus_tag="VGA59_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX9721371.1"
                     /translation="MPSLFQRLMNATRLTRAGDLRAATAAIQAALADGSSTATSTAAG
                     DPHVIDVAAREVDVPSLAAALSHDDAGEGVAAPPPPMPRRSGRGEFVAGSYVCAAGAR
                     DYKLFVPPAAGTRALSLVVMLHGCTQDPDDFAAGTAMNDAALDHGFFVLYPAQSPQAN
                     PQRCWNWFKHNHQARGRGEPELLAGMTRDVMARHAIDPARIYVAGLSAGGAMAAILGD
                     AYPDLFAAVAVHSGLATGVAKDLPSALGAMKGSGARPDGVASGVATIVFHGDADATVH
                     PGNGDHVVAASVGTGHSETDKGVSAGGRGYSRSVHRGPGGEVVAEHWVVHGAQHAWSG
                     G"
BASE COUNT         1928 a         4036 c         3955 g         1974 t
ORIGIN      
        1 aacgcgcgac gcgagcgccg ccgcggcgcc ccaagccggc tccgcgcgcg tgagcgaagg
       61 ccagccgcct cacggccccg aagtcgccat gctggccttg gcctatggac tggccggctt
      121 cggctacatc atcaccgcca ctttcctgcc ggtgatcgcg cggcaatcgc ttcgggattc
      181 cgcatggctc gacatgttct ggccgatcct gggagccggc gtcatcgccg gcgtcttgat
      241 cgcctcgcgc ctgcgcggcc acggcgacca gcgactgcgg cttggggcct gctacctggt
      301 ccaggcgctc ggcatcgcca ccagcctgtg gagtccgacg ctggccggtt tcgcgatcgg
      361 cagcctgctg ctgggcctgc cgttcacggc catcaccttt tttgcattgc aggaggtgcg
      421 gcggatgcgc ccgaccgcgc cgtcgggcct catgggcctg atcaccgcga tgtacggcct
      481 cggccagatc gtaggcccgc cgctggctgc ggcgctggtc gcccgaagcg cgcaccccgc
      541 cgaaggtttt gccctgtcgc tcgaaatcgc cgcgggcgct ctggtgctgg gcgcgttgat
      601 ctacttcttc atggcgcgga tctatccgca agctcatccg caaggacgcc actgagctct
      661 gccgtgcggc tcgcggcgcc cggcctgcaa gaatttcgag ggccgcgtat gctgcgccaa
      721 ttcaataaag ggagcatcga atgccgcagc gcatgaccgc ccatgatttc gaccaggaac
      781 tgctgatcct tttcgatgcc tatgtccatg gcaacctgga tcggcgaggt ttcctcaacc
      841 aggcgcaaag gttcgccaag gccggcgtca cggcggccgg cctgctggcc gcgctcagcc
      901 ccgacttcgc cgcgggccag caggtgcgcc cggacgatcc caagctgaaa accgagcggc
      961 tgagctaccc ctccccggcc ggtagcggca cgatgaaggg ctatctggcc cgccccgcca
     1021 gtgccggcgc gaacaagctg cccgcggtgc tggtgatcca cgagaaccgg ggcctcaatc
     1081 cgcacatcga ggacattgcc cggcgcctcg cgctcgatgg cttcatggcg ttcgcacccg
     1141 acgcgctgac accgcagggc ggctatccgg gcgacgagga caaggcccgc gggctgttcg
     1201 cccggctcga ccaggccaag accgaggagg atttcctcgc gggcgcccgc tggctggaca
     1261 agcgcggcga tgcgagcggg aagctgagcg cgatcgggtt ctgctggggc ggcggcatcg
     1321 ttcacgtgct ggccacgcgc atgccggagc tggacgcggg cgtggctttc tacggcaaca
     1381 ccccggcgcc tgaagaagcg gcgaaggtga aggccgcgct gctgatccag caggccgcga
     1441 tcgacgaaag aatcaatgcc gcctggcccg cctacgaagc agcgctgaag gccgcaggcg
     1501 tgaagtacat ggcataccgg tatccgggca cccagcacgg cttcaataac gacacgaccc
     1561 cgcgctacga tgcggctgcc gccaagctgg catggggacg caccatcccg ttcttgcagg
     1621 cgacgctcgc gacttgaccg cgggccatgg gcgtggcccc tctctcttcg caagaccggt
     1681 ccttgcggcc tcagggccgc agcatgtgaa agttcagcaa ccgggtcgca accactgttt
     1741 ccaccagaaa gcacagcagc gccagcagga aacagaccac gcccagcagg aagctgccca
     1801 cggcgatccg gttgatctgg aatgcggtgg tctcgccgag aaacagcacg atgatggtga
     1861 gcccgatcag gaaggcgcag gtggtcagca gggcgatgca gccgttcgtg agccggccgc
     1921 gcagccgcag ctcctccagt tccgtacggg cgcgctgcag gaattcatcg tcggtgcagg
     1981 tgcgggcgcg gtcttccacc gtgcgcgccc ggtcgatgat gcgtgccagc cggccggcca
     2041 ccgcgcctat cattcccgac acggccgtca gcaggaacac cggcgccacg gcgagctgaa
     2101 ttccatgggt gactgcggcg gcatcgagtg gaaaagccat gtgcgattat gaccagtgac
     2161 ggcaccgggt aagccggacg ggttatcctg atgcatcaga tgaacacccg ccacgccacg
     2221 cccgacaaag agcagatcgc attgctggag ccattcgagc ggctcgggct ggaccgcatc
     2281 cacctggtcg cgaccgcggc gcaggcgcgc agcgcccacg cggaactcgc ggctgcacgc
     2341 gcctggggct tcgacaccga atcgaaaccg accttcgtca aggaccaggt ctcccaggga
     2401 ccgcacgtcg tgcagctgtc cacggcccac aaggcctggg tgttccagct gcatgacatg
     2461 gaatgccgca ccgcggtcgc gaatctgctg tccctgcccg gtgtggtcaa ggccggcttc
     2521 ggcctcgggg atgaccgcaa gcgcatcgcg agcaagctgg gcgtcgagcc ggtggacgtg
     2581 ctggaactca acgccgtgtt tcgcgatcgc ggctaccgaa aggacatggg ggtcaagggc
     2641 gcggtggcgg tgctgttcaa ccggcgcttc atcaagtcgc gcaaggcgac gacctcgaac
     2701 tgggcaaatc cccgattgac cgacgcccag ctgatctacg ccgccaacga cgcttatgcg
     2761 gcgttgcgtg tgttcgacgc gctggacctg gcttaggacc tgctcacacg aagtgaaaag
     2821 gtgcggccag gccctcgctg ctactggcgg ttggggttgt tcggcgcgct gtcattgcga
     2881 ttacgcacgc cgtcgccgtc gcggtcgcgg tcgtcgcgat tgcgcacccc atcaccgtcg
     2941 cgatcgcggc tgcgcgcccc gcgttcccaa tggcccgcct gaaggaccca gacctttcgc
     3001 tccacccagg tgtcagccac ccagtgctgg ccgcggcgct cacgcatcat cttgcccggg
     3061 gcccatgcgt gcttgccgtt gcgccatgcc cagtgacccg gcgcccattc ttggccacga
     3121 cgcatgtccg gcaccgcttc ctcgcgcggc ggcggcgggg cctccctgat ggtgatggtg
     3181 cgctgctgcg caaacgaggt cagcggaacg ctggcgaagg aagcggcaat gacgctcgcg
     3241 aggagaattt tcctgaacat ttgcttggtc tccaaaggac tggctgagcg tcaaaaacga
     3301 aacccatctg gccaacgtcc agagtggacc gggattgagg ccagcaccgt aggacaagcg
     3361 cgctttatgg tcgagctcct ggctgattcg gggtgttcag ccaccgaccg gtgccgcgcc
     3421 ccccgccaga gcgtcgtagt aacggaactc atggcagttg ctggcccagt ggcgattggt
     3481 ggatttgcgc gcgcccacgc caaccaatgt gcggcccgcc gccctgatgc tctgcgcgag
     3541 cggcgtgaag tcgctgtcgc cgccgaccac gacgacggtg gctatgtgct cgaagcgccc
     3601 gaggtgctcc atcgcgtcca ggcacagcct gatgtcggcg ccgttcttgg acgaaccgcc
     3661 gggcggaaag agctgtatca gttcgatccc gttctgcagc aggacgtcgc ggtagcggcc
     3721 gaagtactgc cagttgcagt aggcgcggtc gatggcgatc gggccgaacg atgcggccag
     3781 atcgacgatg gcctgcacgt cgatcagcgg ctcctggacc ttgaagcggt tgtcggactt
     3841 gctgccgtag gcgccctcgc cctgcgcctc ttcatagagg ctggcgtgca ggttctcgaa
     3901 gtcccagtac agcgccacgg aaccgccttg cttcccggtt tccatgaggc gctcagccgc
     3961 gtgtgcgggg gtgaagcgga taggccggat cgttgtaccc cggcgtcgac ggatgaccgg
     4021 gcctgacgaa cgaatcgacc agtgcctcgt cctcgcctgt cacgacgtag tccagcgcgg
     4081 gcaggtagtc ctgccactgc cgcagcgtgc gcggcccggc gatcacggaa ctcacggccc
     4141 ggtgcgcgag cacccaggcg gtggcgaact gcgcgagcgc gatgcccttg ccctgcgcat
     4201 gcgcctccag cagttgcgcg atccgcagcg actcttcacg cagctcggtc tccgcgatac
     4261 gctggtcgcc gcgtgccgca cgcgagcctt cggccggcgg ctggcccggc cggtacttgc
     4321 ccgtcagcac gccgcgcgcg atcgggctat agggcgtcac gccgatgcca tagtgctcgc
     4381 acgcgggcag gatctcgact tcgggcatgc ggttcagtag gttgtagtac ggctggcaca
     4441 cggccggacc cggcatgccc agctggccgg cgacgcgcat gatctccgcg atacgccagc
     4501 cgcggaagtt ggacacgccc caatagcgga tcttgccggc ccgcagcatc gcgtcgagcg
     4561 cccgcagcgg ctcctccagg tccatgccgt tgttgtcgcg gtgcatgtag aggatgtcca
     4621 cgtgatcggt ctgcaggcgc ttgaggctcg cctccacctc gcgcagcatc catgtgcgcg
     4681 agaagtggcc ctcgttgacg cgttcggaca tcttgttgcc cagcttcgtc gccagcaccc
     4741 agtcgtgacg ctgtcccttg agcagctcgc caaccatcga ttcggacgcg ccgttggtgt
     4801 acacgtcggc ggtatcgatg taattcacac cgtgctcgcg cgcatcggcc agaatggcct
     4861 gcgcctcgtc gcggcccgtc tggtcgccga acatcatggt gcccaggcac agggcggaaa
     4921 ctttcaaatt gctcttgccg agctggcggt attgcatggc ggcctccttg gggcccacag
     4981 cttaacgggt gtcgcgattt cgcgcgcggc gccgcagggc aggcgctcag tcgaattcca
     5041 cgcagcccgg tgctcagcgg ccccgagccg ccggcacgga agcgacgcag ccggcgtttc
     5101 tcaagataca gccgatgcac atgaagacgc agcccatgtt cgcggagacc caaccgctgc
     5161 cgctggacgc agacccgccc gcttaacggc ggacctgatt gctggcaatc accaccgcgg
     5221 attcattggc gcgcttgaag ttcatcgccc gcagctgcgc gtcgaagaat tccgcctgtt
     5281 gggcgccgac gcgggcgttc tggatatgaa gctgggtttc ccgcggcagc ttgccggtgt
     5341 tgcggccgtt ccaggtattg cggccgcggg tggaaaggtt gtaatagatc gagatgcctt
     5401 cgcgcagctt cgcggcgcgc tcgtcggcgc cgagtttcag gcccgcgatg cgattcgcgc
     5461 tccagatcgc cgcttccttc atgtgcaagg cggccacgtg caccgcttcc acggcatctt
     5521 cgggattgcg catgcccagc gcccgggccg tggcaggctc gaattgcgcg atgcccaggt
     5581 tgggcgtgcc gtccttgctg gcgcccatgc gcggaatcca ggaggtttcg gcattgatga
     5641 tgccgtacac gtccttgaag cccagcccga gcgcgctgag ccccgcgcgg ctggcggccg
     5701 acttcgccag cagcaacctg gaatcgaggt cgggtgtgga gaacttctgg ctgccgacgc
     5761 gggtcgggac ccagcgaacc ctggaccgca agtgggtgta atagcggtcc tgcggtgaaa
     5821 ccggcgccgg gcttgccgca gccagttgcg gctgcgatgg aatcggcaca acgttgcctg
     5881 ctgcagagtc ggcggttgct gccgggccgg ccactgtcgg gctggcaggc gcctgcgccg
     5941 cctcgacggt gggctggctg ggctggaccg aaaccaccgg tttgttggcc gtcaggggct
     6001 cgctcacccg cgccggcact tgcatccaca gcgctacgag gcatccgagc ccgaccgtaa
     6061 aaaagccggc aacagcagag taaacaggcc agttctggcg agtcatgtag tccccttctt
     6121 acaaagctgt tcaagggacc gccatggcgt cgcgaaagtt caccgctgtt tcaattgaat
     6181 ctcccgactg tcgcgcgcag gccaactatt ccacaggctc gcgatcacga accggtcaag
     6241 gagggtttgc ggcgtggatg ccggccagca cgctggcaac gatgcggtcg cagcgggcgc
     6301 cgtcgaatga gaaggcgtct tcgagcgcga aatccatgtc gcgcgacagg ctctcgcgca
     6361 gctgcgcgcg ggcccggaag aagcgagtgc gaaccgtggc ctcagggagg tcgagcgccg
     6421 tggagacttc ctccacgctc atctcttcca ccgcgcgcaa catgaacacg gtgcggaaca
     6481 actgaggcaa ctcgtcgatg cgcgcttcca tcagccgccg catttcggcg cggctggcca
     6541 cgaggtccgg ctgttggtcg tggtcggctt ccatcgtggc ctgcgttcgt ccctcgccaa
     6601 ggggcgccgc cgagtcgaac atgaccacgt tggacgcacg gcgtcgcacg cgtttgagcg
     6661 cctcgttcag cacgatgcgc accagccagg tggacagctt cgcgtcatcg cggaaggtgt
     6721 gcagggcgcg ccaggcattc aggtacgcgt cctgcacggc atcttcggtt tcggcgtcgc
     6781 ttctgaggat gctgcgggcg gtgcgaaaca gcagccggtt atgacgccgc atgatcgctt
     6841 cgaacgcgcg ggggtcgccg gcggtggcaa gggcggccag ttcggcgtcc ggcgtcttgt
     6901 cggacagcat ggcggcgctt cgtgggctgg ggctttgcat atcggttctc gggcattgga
     6961 tgcagccggg acccgccgcg ttcccgcgcc gtttccgggc tacgaaaccc ggaccatgaa
     7021 atccgggtcg aaggcggcgt tttcattcac gccgccgcgt gcataaccgc gggggttgca
     7081 cacgaccctg gtgcccttca cgacgtagtc gaagctgtca tgggtgtgcc cgtggatcca
     7141 gagctgggcg cggtcgccgt ccagcaggta atcggcgtcc gacacgaagc aggcgttgac
     7201 cagcgacccc tcgaaacgcg gatgaatact tgcccgcgac ggagcgtggt gcgtaatgac
     7261 caccgtcggg ccctcgtgcg gttgcgccag ttcattcgcc agccatgcgg cgtggcgctg
     7321 gaacagcgac accgaatcca gcggcgagaa cagcgcctgt gaagcgtcgt cgacgcgaat
     7381 gcggctgaag tcgcgcatga gccgcaccgc ctggtccatc gcggcctgcc gcccctggcc
     7441 ctcgccgaac agcatgaagt cggtccataa cgtggacccc aggaatcgaa cattaccgat
     7501 gagctgggtc tcgtcgtcga gaacatggat ggacgtgcct tcgcacaatt gcttgagctg
     7561 cgccaccgag ctgcggaggc tgccgccgta gaactcgtga ttgcccggca cgtagagcac
     7621 cggcttgccc aggcgagcgg cccacgcaat ggcttcgggc ggccgggcga catcgccggc
     7681 gaggaccacc acgtcggcgt cgttctgcgg cagggccaac tcgccctggc tcaggtgaag
     7741 gtccgacagg atgttgagtt tcatggttgg cggcagggac ggagcgcggc tgtctatgat
     7801 ggcatgacat atccggccgc ggcggccggg aaaccgacat gacattgtcc tcatcctttc
     7861 aggccgacgg ggcgttggag ctgctgaccg acctgcacgc gcgcacgctg caccagtgcg
     7921 cgtcgctgcg gcggctggca cacgacctgg ccgaacgtgg ctgcgacgac caggcgcgca
     7981 gcgccgccga aaccttgttg cgctacttcg acgtcatccc tccccggtac tacagcgacg
     8041 aggaggaaga cctgtttccg gcactgctgg aatcgatggc ggggtccgac gccgtctgcc
     8101 tgcgggacct cacggccggc atggcacggc agcaccgcgc gctcgagacg atgtgggagc
     8161 gcttgcgcaa ggcgctggcg gcggtggcga acggacagca cacattgctc gacagcgccg
     8221 aggtcgaagc cttcgccgcg ctgaacgaaa gccacatcga gcgagaacag gccgagctgc
     8281 tgcccatggc gcagcgcctg ttgaccgacg acgcgctggc gcagatcggg gcctccatgc
     8341 gcgagcggca tcgcgtggac tagcgctttc gcagcggatc cagcagcggc ttgagcccgt
     8401 tgtggtcgat ctcgtgcatg agcgccagca ggcgcctgtt cttccccacg gctgtcatcc
     8461 cgggctcgac ccgggatcca tcccggcacg cccgccgcgc ctcttgccgc catggattgc
     8521 gggtcaggcc ggtcaggccg gtcaggcccg caatgacagg agatggctgg tccatgcccg
     8581 gcttaccctc cagcccgctt gacggtgtgg ccctgcgcct tgagcatctc caccacgcgg
     8641 acacagtgat cgccctgtac ttcgatcacg ccatccttca ccgtgccgcc ggagccgcag
     8701 gccgccttca gctgccggcc caccttgccc agcagggtcg cgtccagcgg caagcccttg
     8761 accaccgtca ccgccttgcc gccccggccc ttggtttcgc gcgagacgcg gacgatgccg
     8821 tcggacggcg cagccggctg cctggcttgc gcgcacacgc actgcgcgac cggccgccgg
     8881 catgccgggc acatgcgtcc ggcgtcggtc gagtacacca gaccaccggt gccggccttg
     8941 cttttcatcc atgcagttta gggcctgatc acactgtttt taccagtgca aaaacagtgt
     9001 gaactggcac tagcgcaatc gacctacacc ttgatttgcg cggccgcctt ccggtccgcg
     9061 accacctttt gtgccgaagg ccgctgcgca atcagccggg tgtaagcctt gtgatcgatg
     9121 ccgccggcgg ccagcaggtc ttcgccgaac acgagtttcg tggccagcgc caccagcggc
     9181 aggtggttga aagccgagca gtcggcctgg gtgaagctat cgcccgccac gtagggtgcg
     9241 aacttcgcca gccgcttgaa ggccgcgatg tgggccacga gctgcttgcg gatacgcgcc
     9301 gcgttggctt cgctcaaggg cgccgcgccg aaaaaggccg ggccgtacag ctggcgcgcc
     9361 acgatctcca ggtgccagtc gatgaaggtg gtgagctcgc gcaccttggc ggcggcaaac
     9421 gcttcggcgg gcagcagcgg cggctgcggc cagctgtcct cgatccactc cagcagcggc
     9481 tggctttcgc acaacgcgcc ctgccccgtc gtgatgaacg ggatcttgcc cagcggcgac
     9541 gcgccgagga tctgctcgtc cgtgctgtgc gtggccgcgt acgcttcggc gaacggcacg
     9601 cctttttcca gcagcgcgag cttgaccttg ttgtagtagt tggaaatggg cgagccgtga
     9661 agggtcagca tgggaaggtt tccggtgaaa cgattacttt aagcggcggc gcccgccgtc
     9721 attggcaccc gcgagacacc cgcgcgtgcg actcagccgc gccgcttgag cggctgccag
     9781 ccggcgaacc gcgggtaatg gcgctgcacc accggcccgt cgaaatccca ggcgatgcat
     9841 ttgcccaggt ggtaaggcgg cttgtcgcca tcgggctccg gcacgccggc gagcctgcgc
     9901 aggcggtgct ggtaggaagg aatggtctgg tacgccgccg tggccatgtt gaacagcagc
     9961 tcacgcaggt gcgtgcagcc ccggaccccg cccagcgcct tttcgatcgc ctggcgccag
    10021 cccgggccca tcgtcgcgcc caccatgcgc tgcatcggct ccttgccctg ctggcattcg
    10081 tcgaaaggcg tgtggtccga ggaactggcg atctcgcgaa tcgtcagggt gtcgtcgacg
    10141 gttacgcgga tggacatccc gtggatgggc gttccgggcg gcatgaggcc ccgttcggaa
    10201 cgctccagcg gataggtctt cgtgtcggtc atatccgcct cgatgtccca caggccgtcc
    10261 tctcggtgat agccacggta gacgacggcg cgggtgtgca ggtgggttcg ggcttggggc
    10321 gcgggcagag gcatgaggta ctttgctgga acgggggaga aggcaggatt ttctcaggtt
    10381 gcggataacc gcgtgacggg cttccaccat ccacttggat ggtgctaaga tgccacccat
    10441 gcccaaagcc agcagcaagt ccctggtcgc ccagcccagg cccacggacg agaaaatcac
    10501 cgtcaacctg ggctgtgtcg acctcgggca gatcgatctg ctggtgcagg aaggcttcta
    10561 tgccaaccgc accgacctga tccgcaccgc catccgcaac cagatcgcca cgcacggcga
    10621 cgcgctgcgg caggcggtgt cgcgcaagac actggtcctg ggcatccagc acttcagcaa
    10681 ggccgacctg caggcggtgc aggccggcgg caccaggttg cagatccgcg tcctgggcct
    10741 cgcgtcgatc gcccccgacg tcacgccggc gctggcgctg gccaccatcg aatccatcac
    10801 cgtgctgggc accctgcaag ccagcgccgc cgtcagggcg gcgctcacaa agcgcatccg
    10861 ctaacacact ccgatatgaa aaaccccatg ccctccttgt ttcagcgcct gatgaacgcg
    10921 acccgcctca cccgcgcggg cgacctccgt gccgccaccg ccgcgatcca ggccgcgctg
    10981 gcggacggct cgagcacggc aacgtccacg gccgccgggg acccgcacgt catcgacgtg
    11041 gccgcccgtg aagtcgacgt gccctcgctc gccgcggcac tttcgcacga cgacgccggc
    11101 gagggcgtcg ctgccccgcc accgcccatg ccccgccggt ccggccgggg agaattcgtc
    11161 gcgggcagct acgtctgcgc ggccggggcc cgcgactaca agctctttgt tccgcccgcg
    11221 gccggtaccc gtgccctttc cctggtggtg atgctccatg gctgcacgca agaccccgac
    11281 gatttcgcgg ccggcaccgc gatgaacgac gccgcgctcg atcacggctt tttcgtgctc
    11341 taccccgcgc agtcgccgca ggccaacccg cagcgctgct ggaactggtt caagcacaac
    11401 caccaggccc gcggccgcgg cgaacccgag ctgctggccg gcatgacgcg cgacgtgatg
    11461 gcacggcatg ccatcgaccc ggcgcgtatc tatgtggcgg gcctgtcggc cggcggcgcc
    11521 atggccgcga tcctgggcga cgcctatccg gacctgttcg cggccgtggc cgtccactcg
    11581 ggcctggcca ccggcgttgc caaggacctg ccatcggccc tgggcgcgat gaagggcagc
    11641 ggcgccagac cggatggcgt ggccagcggc gtggccacca tcgtcttcca tggcgatgcc
    11701 gatgcaaccg tgcacccggg caacggcgat cacgtcgtcg cggccagcgt cggcaccggc
    11761 cacagtgaaa ccgacaaagg agtttccgcg ggtggccgcg gctactcccg tagcgtgcac
    11821 cgcggccccg gcggcgaggt cgtcgccgag cattgggtag tgcacggcgc gcagcacgcc
    11881 tggtccggcg gca
//
LOCUS       DASWIY010000468         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1839109, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000468 DASWIY010000000
VERSION     DASWIY010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839109"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VGA59_16765"
     CDS             <1..231
                     /locus_tag="VGA59_16765"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX9721372.1"
                     /translation="FELPVLFYAAVLLSLVLMIQDAQLVRLAWGFVLLRIVHSVIHCT
                     YNNVSHRFVAYALSSLFLLFMWIRLGAYILTH"
     gene            241..645
                     /locus_tag="VGA59_16770"
     CDS             241..645
                     /locus_tag="VGA59_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX9721373.1"
                     /translation="MSDKETVQGGCLCGAVRFALELPSKWCAHCHCSMCRRAHGAGYV
                     TWAGFASEHFRLLQGDHQLKWYESSPGARRGFCSACGSTMLFESTRWAGETHVALGCV
                     DGPIDRPPQAHAFYDTHVDWIAIDGSLKIYPG"
     gene            complement(719..1399)
                     /gene="ung"
                     /locus_tag="VGA59_16775"
     CDS             complement(719..1399)
                     /gene="ung"
                     /locus_tag="VGA59_16775"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9721374.1"
                     /translation="MDKTRQIRLEPNWKARLETEFEQPYMVALRQFLVERKRAGAIIY
                     PPGPLIFNALDSTPFDDVKVVILGQDPYHGPGQAHGLCFSVQEGVALPPSLLNIFREI
                     RDDLGRAPPAGGNLQAWAEQGVLLLNAVLTVERGQAGAHQGKGWERFTDRVVQELNAG
                     REGLVFMLWGSQAMKKGARIDRDRHLVLSAPHPSPLSAHRGFFGCRHFSKANTWLAQH
                     GQEPIRWA"
     gene            1481..>1793
                     /locus_tag="VGA59_16780"
     CDS             1481..>1793
                     /locus_tag="VGA59_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX9721375.1"
                     /translation="MPTETTITTDVVIIGAGPTGLFAVFELGLLDIRAHVVDSLRQVG
                     GQCSELYPDKPIYDIPALPVCGAQELVDRLMEQIRPFQPEFHLGQTVTVLERTPDGRF
                     LL"
BASE COUNT          325 a          587 c          557 g          324 t
ORIGIN      
        1 ttcgagctgc cggtgctgtt ctacgcagca gtgctgttgt cactggtgct gatgattcag
       61 gacgcgcaac tggtgcggct ggcctggggc ttcgtgttgc tgcgtatcgt ccacagcgtc
      121 attcattgca cctacaacaa tgtcagtcac cgcttcgtcg cctatgccct cagcagcctg
      181 ttcctgttgt tcatgtggat ccggctcggc gcgtacattc tgacgcactg aggcgcagcg
      241 atgagcgaca aagaaacagt ccagggcgga tgcctgtgcg gcgcggtgcg cttcgctctc
      301 gaactgccga gcaagtggtg cgcacactgc cactgctcaa tgtgcaggcg cgcccatggc
      361 gccggctacg taacctgggc gggcttcgcc agcgagcact tcagactgct gcagggcgac
      421 caccagctca agtggtatga atcttcgccg ggcgcacggc gcggattctg cagcgcttgc
      481 ggcagcacca tgctgttcga gtccacgcgt tgggccgggg agacgcacgt cgcgctgggc
      541 tgcgtcgacg gaccgatcga ccgcccgccg caggcccacg cattctacga cacgcacgtc
      601 gattggattg cgattgacgg gtccctgaag atctatcccg gctgagcgcg gcgaaggcaa
      661 cacgatgcag ctggcatgca gctggcatgc agcaggccgg gctcgcccgg catggccgtc
      721 acgcccagcg aatcggttcc tgcccgtgct gtgccagcca ggtgttggcc ttggaaaagt
      781 gccggcagcc gaaaaagccg cggtgcgcag acaacggcga agggtggggc gcggacagca
      841 ccaggtggcg gtcgcggtcg atgcgcgcgc cctttttcat cgcctgactg ccccacagca
      901 tgaacaccaa cccttcgcgg ccggcgttca gttcctgcac aacacgatcg gtgaaccgct
      961 cccagccctt gccctggtgc gccccggctt gtccgcgctc gaccgtcagc acggcattga
     1021 gcagcagaac accctgctcg gcccaggctt gcaggttgcc gccggcgggc ggcgcgcgac
     1081 cgaggtcgtc gcggatttcc cggaaaatat tcaacaggga cggcggcaac gccacgccct
     1141 cctgcacgga gaagcacaga ccgtgagcct gcccggggcc atggtaggga tcctgtccca
     1201 ggatgacgac cttcacgtcg tcgaagggcg ttgaatcgag cgcgttgaaa atcagcggac
     1261 caggcggata gatgatggcg cccgcccgct ttcgctcgac caggaactgc cgcaatgcga
     1321 ccatgtacgg ctgctcaaac tcggtttcga gccgcgcctt ccagttcggt tcgagcctga
     1381 tctgcctcgt tttgtccata gcaacttgaa tctgcccgga tcagcccaag ttagcagaac
     1441 agacagcggt acaaaaccct cctctccaag cgaaccgaaa atgcccacgg aaacaacgat
     1501 taccactgat gtcgtcatca tcggcgccgg cccgaccggg ctgttcgcgg tgttcgaact
     1561 cggtctgctc gacatccgcg cccacgtcgt cgattcgttg cggcaggtcg gaggccagtg
     1621 cagcgaactg tacccggaca agccgatcta tgacatcccg gccttgccgg tatgcggggc
     1681 gcaggaactc gtcgaccggc tgatggaaca aatccgccct ttccaaccgg agttccacct
     1741 gggccagacc gtgaccgtgc tggagcgcac gccggatggc cgcttcctgc tcg
//
LOCUS       DASWIY010000469        10739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_323896, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000469 DASWIY010000000
VERSION     DASWIY010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10739
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323896"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VGA59_16785"
     CDS             <1..1155
                     /locus_tag="VGA59_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEX9721376.1"
                     /translation="LRYWVEEMHVDGFRFDLASALARESGKVEDLSSFFNAIRQDPML
                     AHAKLIAEPWDLGHGGYQVGNFPPGWAEWNDRYRDGMRSFWRGDAGTLGEVARRLTGS
                     QDLYGWSGRPPCASINFVTAHDGFTLHDLVSYDEKHNEANGEENRDGHSHNLSWNCGV
                     EGPTDDASILELRERQKRNLLATLLLSQGVPMLLAGDERGHTQQGNNNAYCHDNELAW
                     LDWTADPRQQSLTAFVRRLIALRRAHPSLRRRRFFNGSPLDGDADKDVCWLRRDGLEM
                     APDHWHEGEARCLGMSISGNGISGPGLRGERLRDDDFLVLFNAGPDDIWFALPKAGDR
                     AWRLLLDTARDDHIHDDPVPAAVLASLHRQDTYPLQNRSVVLLTRPGSPG"
     gene            1152..3008
                     /gene="treZ"
                     /locus_tag="VGA59_16790"
     CDS             1152..3008
                     /gene="treZ"
                     /locus_tag="VGA59_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX9721377.1"
                     /translation="MRRAHRMPFGAQVLAGGGVAFGLWAPALGRVLLAHGAEESAAAH
                     PMAREPDGWHRLELPGARAGDSYRFVLPDGLRVPDPASRFNPGDVHGASRVVDPHAYA
                     WQDRNWRGRPWHEAVVYELHVGAFTPEGTFQAARGRLAQLAGLGITAIELMPVADFPG
                     RWNWGYDGVLPFAPDASYGSPDDLKELVDTAHGLELMMLLDVVYNHFGPEGNYLHAYC
                     PQFFNAAHQTPWGAAINFDGNQARTVRDFFVQNALYWVEEFHFDGLRMDAVHAIRDDS
                     PVHIVREICQALHGGPGRTRNVHVVLENDDNRASFLERGRDARPLAGTAQWNDDLHHA
                     AHVATSRETDGYYADFADAPVARFARALAQGFIYQGEPSRFRGGQPRGEPGAHLPLAA
                     FVSFLQSHDQIGNRAFGERIDRLGDPVLLGAAYACVLLSPHIPMLFMGEEFAASTPFQ
                     YFCDFGGDLGQAVSRGRREEFGRFAAFADEDARSRIPDPNAESTILASKLRWAERGQA
                     PHKDWLDWIQRLLQTRQRLLVPLFAGKCGAGQFQCAGESFSIDWTLGRGGTGAEPARL
                     RLLANFGASALGAVTLPPGSVVYSAGLASDGVGALRLERGGVHVALQEAGDA"
     gene            3001..8112
                     /locus_tag="VGA59_16795"
     CDS             3001..8112
                     /locus_tag="VGA59_16795"
                     /EC_number="2.4.1.25"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX9721378.1"
                     /translation="MPDQEAGGEAQALARLCAHFGIATEYDDIWGTRHRVAPEILRAL
                     LSQFDVAVGDLRTATQALEELSGAQWAAPLPAVVAIPADSDGWSVTLRLAASQDRVRW
                     RLREERGAVHEGEAAVQDMAELSRSGAEGGYRSERRLTMALRLGPGYHQFSIEDPPGE
                     TLVVSAPQQCWRPAALREEGKIWGPAVQLYALRSRRNWGIGDFSDLSALVAEAAGRGA
                     GIVGLNPLHALFPHNPAHASPYSPSSRRHLNVLYIDVEAVSDFAVCEPARRLVRSPEF
                     QARLARLREAPLVDYAGVAEAKFEVLELLYACFAERHLNPDGSAADDTGAEFASFVAQ
                     RGEALFRHALFEALQAHFHAADQAVWGWQVWPEPFRDPAGTEVAAFAVRHAGRVRFHQ
                     YLQWQAALQLARAAHHAEVLGMGVGLYLDLAVSVDRAGSGAWSARDCFAEQAGVGAPP
                     DEFNLHGQGWGLPPLRPDRLRATRYRLFVETLRANMVGAGALRIDHVMGLMRLFWIPA
                     GHPPADGTYVHYRLQELLSIVALESQRNRCMVIGEDLGTVADEVRTALASAGVLSYRL
                     LYFERGAGGDFKSPADYPRDALVAVGTHDLPTLPGWWCGHDLQLRQRLGLFPSQPSFE
                     QQLLERAQDRVKLLLAVQRAGLLTPEDVAAAAGAQAPATGGVEAIHAFLAQAPSAVMM
                     VQLEDVLGQTEQANLPGTTNEHPNWQRKLPLELEALPTEVRWLGLGRRLATLRPRRAP
                     QPVEPGGTSARVPRATYRLQLHREFGFDDAIRILPYLDRLGVSHVYCSPVQRARAGST
                     HGYDVVAHDEINPELGGAQGLRRFIDALRERGMGLLLDMVPNHMGVLGDDNAWWMDVL
                     ENGPASVYAQHFDIDWQPLNRELEGKVLLPLLGDHYGDALTRGEIVLQFEPAGGTLVL
                     RYFEHRFPLAPDTYPWVLDRAQARLGDTDLSAGLASIATAFGHLPGRAADSAQARAER
                     DRDKELLKARLARLAARQPAVAQAIAASVAQLNLASARDELHALIEAQAYRLAYWRVA
                     ADEINYRRFFDISELAALRIERDDVFETTQSFALDLAAGGAVDGLRIDHPDGLNDPAR
                     YFAKLQAGYAARAGLVLPERDSQGRPSRPLYVVAEKIAAGHEEVPESWHVHGTTGYRF
                     ANVANGVLVDTAAAARFVQIWHDFTGQRQTFAEVARVSKRDIMRTALASELNVLSAEL
                     LRIARADRRTRDYTLNALRRALADVVACLPVYRTYIIDAPSQQDLHYVNWAVKEAERH
                     SEDADLSVFAFLRQTLLGQCVPDAPAELSARVRRFATRLQQFSAPVAAKGVEDTAFYR
                     YFPLSSLNEVGGEPAQFGMTVPQFHEASADRCRRWPHTMLATSTHDHKRSEDVRNRIN
                     VLSEMPAAWRLALRRWRSLNRGIRDRLVAAGAPADAPSAADEYLLYQTLLGTLPPEPA
                     DAPLRQAWRDRVVRYMLKAAREAKLHTRWTHPDEAYEAALEGFVDGLLSPAGGASAFL
                     TDLHALGARVAWFGALNSLSTTLLKHTSPGVPDLYQGHEVINLTLVDPDNRRPVDYER
                     LGQALADLQAMDPGALAALAAAPANGLAKLWIAWRLLRLRRQQPDLFRSGDYQALETG
                     GRHSGHVLAYARRNEGMTLVVIAGRLWSGLPAEAGTGPLGEAVWSDTWVAVGLPHGTA
                     LTDVLSGRDLTVENGRIAVGRAFSCLPAAALVCLA"
     gene            8123..8593
                     /gene="nudB"
                     /locus_tag="VGA59_16800"
     CDS             8123..8593
                     /gene="nudB"
                     /locus_tag="VGA59_16800"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEX9721379.1"
                     /translation="MADGSPFKIPESVLVVIYTPALDVLLVDRTDAPGFWQSVTGSKD
                     SANETLAQTAVREVFEETGINCGVGTTLHAGLKDWGLENIYDIYPRWLHRYAPGVVRN
                     TEHVFGLCVPPGTPVTLSPREHTGYRWLPWREAADACFSPSNAEAILLLPQFAG"
     gene            8664..9392
                     /locus_tag="VGA59_16805"
     CDS             8664..9392
                     /locus_tag="VGA59_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX9721380.1"
                     /translation="MPVLRVATYNIHKGVQGVGPGRRLEIHNIGHAVEQLDADIVCLQ
                     EVRKLHRREQRYFTRWPELPQAEFLAPEGYASVYQTNATTRHGEHGNALLSRWPVVSH
                     GHEDMSDHRFEQRGLLHVEVHAYRRPIHVIVLHLGLIAGSRLRQIEQLGLYIQREVPR
                     REALVVAGDFNDWGGKLRSAMDGYRLKDFVGERLLTYPARLPLAQLDYIYARGLKPLG
                     VQIPRGRIWWRMSDHLPLIGEFKV"
     gene            9397..10611
                     /gene="clsB"
                     /locus_tag="VGA59_16810"
     CDS             9397..10611
                     /gene="clsB"
                     /locus_tag="VGA59_16810"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEX9721381.1"
                     /translation="MSHASSVPRGGHDLQLLQGSGELFPALVEAIDSARREVRLETYI
                     FDFTGHSAEIAYALERAARRAVSVKVVVDGFGSAPLPPAWAERFAQAGVEWRVYAPLG
                     WLGILWVGNWRRLHRKLCVVDGQIAFCGGINILDDFHDPNHGPLESPRLDFSVRVTGP
                     LVAEVHAAMTRLWLRMQAMSQARRARLADMLGSLREYGLGRARRPAPEPGQRRSRAAL
                     VLRDNLRNRARIERAYRRAIGRAREEIIIANAYFVPGRKMRKALVSAANRGVKVRLLL
                     QGRYEYFMQYYAARPIYGALLRAGVEIYEYSPSFLHAKVAVIDGRWATVGSSNLDPLS
                     LLLAREANVIVEDEDFAARLRRHLLRAIRAEDQAMDPEGFRQRPLRQRFMEGVALALM
                     RLALAIQGKKYL"
BASE COUNT         1615 a         3633 c         3786 g         1705 t
ORIGIN      
        1 ctgcgctact gggtcgagga aatgcacgtc gacggcttcc gcttcgatct tgcctctgca
       61 ctggcacgcg agtccggcaa ggtggaagac ctgagcagct tcttcaacgc catccgccag
      121 gatccgatgc tcgcccacgc caagctgatc gccgagccct gggacctggg gcacggcggc
      181 taccaggtcg gcaacttccc gcctggctgg gccgagtgga acgatcgcta ccgcgacggc
      241 atgcgcagct tctggcgggg agacgccgga acgctgggcg aagtggcgcg gcggctgaca
      301 ggctctcagg acctctatgg ctggtccggc aggccgcctt gcgccagcat caacttcgtg
      361 accgcgcacg acgggttcac gctgcacgac ctggtttcct acgatgagaa gcacaacgag
      421 gccaatggcg aggagaaccg cgacggccac agtcacaacc tttcatggaa ctgcggcgtg
      481 gaagggccca ccgacgatgc gagcatcctc gagctgcgcg agcgccagaa gcgcaacctg
      541 ctcgccaccc tgctgttgtc gcagggcgtg ccgatgctgc tggcgggcga cgagcggggc
      601 catacccagc aaggcaacaa caatgcctac tgtcatgaca atgaactggc atggctcgac
      661 tggacagcgg atccgcgcca acagtcgctg acggcattcg tgcggcggct gattgcgctg
      721 cggcgcgcgc acccgtcctt gcggcggcgc aggttcttca acggcagccc tctggacggg
      781 gatgcagaca aggacgtgtg ctggctcagg cgcgacgggc ttgagatggc tcccgaccac
      841 tggcatgagg gcgaggcccg ctgcctgggc atgtcgatct cgggaaacgg aatttcaggc
      901 cccgggcttc ggggcgaacg acttcgtgac gatgattttc tggtgttgtt caacgccggc
      961 cccgacgaca tctggttcgc cctgccgaag gctggtgacc gcgcatggcg gctgctgctg
     1021 gatacggcga gggacgacca cattcacgac gaccctgttc cagccgccgt cctggcttcg
     1081 ctgcaccggc aggacaccta tccgctgcag aaccgctcgg tcgttctgct gacccgtccc
     1141 gggagtcccg gatgagacgc gcgcaccgga tgcccttcgg cgcccaggtg ctggccggcg
     1201 gtggcgtcgc cttcggcctg tgggcgccgg cgctgggccg cgtgctgctg gcccatggcg
     1261 cggaggaatc cgccgccgcg cacccgatgg cgcgggagcc cgacggctgg caccggctcg
     1321 aactgcccgg cgcgcgggcc ggcgacagct accggttcgt gctgcccgac ggcttgcgcg
     1381 ttccggaccc ggcctcgcgc ttcaacccgg gggacgtgca tggcgcgagc cgcgtcgtcg
     1441 atccgcacgc gtatgcctgg caggaccgca actggcgcgg ccgcccctgg cacgaggccg
     1501 tggtctacga actgcacgtc ggcgcgttca ctcctgaggg cacgttccag gctgcgcgtg
     1561 gccggttggc gcagctggcc ggactcggca tcacggcgat cgaactgatg ccggtggccg
     1621 actttccggg ccggtggaac tggggctacg acggcgtgct gccgttcgcg cccgacgctt
     1681 cctacggctc gccggacgat ctcaaggagc tcgtggacac ggcccacggc ctggagctca
     1741 tgatgctcct ggacgtggtc tacaaccatt tcggccccga aggaaactac ctgcacgcgt
     1801 actgcccgca gttcttcaac gccgcccacc agacgccgtg gggcgccgcg atcaacttcg
     1861 acgggaacca ggcgcgcacc gtgcgcgact tcttcgtgca gaacgcgctt tactgggtgg
     1921 aggagttcca cttcgacggc ttgcggatgg acgccgttca cgcgattcgc gacgacagcc
     1981 cggtccacat cgttcgcgag atctgccagg cgctgcacgg cgggcccggc cgcacccgga
     2041 atgtgcacgt ggtgctcgag aacgacgaca atcgcgcctc attcctggag cgcggtcgcg
     2101 atgcgcggcc gctcgccggc accgcgcaat ggaacgacga cctgcatcat gccgcccatg
     2161 tggcgacgtc gcgggagacc gatggctatt acgccgattt cgccgatgca ccggtggccc
     2221 ggttcgcgcg cgcactggca cagggcttta tctaccaggg cgagcccagc cgctttcgcg
     2281 gcggccagcc gcgcggagag ccgggcgccc atctgcccct cgcggcgttc gtctcgttcc
     2341 tgcaatcgca cgaccagatc ggcaaccggg ccttcggcga gcgcatcgac aggctgggcg
     2401 atccggtgct gctgggcgcc gcctatgcct gcgtgctgct gtctccgcac atcccgatgc
     2461 tgttcatggg ggaggagttc gcggcgtcca cgccgttcca gtatttctgc gatttcggcg
     2521 gggatctggg gcaggcggtg tcgcgcggcc ggcgcgagga gttcggccgc ttcgcggcgt
     2581 tcgccgatga agacgcgcgc tcgcgcattc ccgaccccaa cgccgaaagc acaatcctgg
     2641 cttccaagct gcgctgggcc gagcgcgggc aggcgcccca caaggactgg ctggactgga
     2701 tccagcggct gctgcaaacc cgccagcgcc tgctcgtgcc gctgttcgcg ggcaagtgcg
     2761 gcgccgggca atttcagtgc gcgggtgaaa gcttcagcat cgattggacg ctcgggcgcg
     2821 gcgggacggg cgcggagccc gcgcggctgc gactgctggc gaacttcggc gcgagcgcgc
     2881 tcggcgctgt gacgctgcca ccgggcagcg tggtctattc ggcgggcctc gcctcggacg
     2941 gcgtcggcgc gctgcggctg gagcgaggcg gcgtgcacgt ggcgctgcag gaggcgggcg
     3001 atgcctgacc aggaggccgg cggcgaagcg caggcgctgg cgcggctgtg tgcgcacttc
     3061 ggcatcgcaa ccgaatacga cgacatctgg ggcacccgcc atcgcgtggc gcccgaaatc
     3121 ctgcgcgcat tgctttcgca gttcgatgtc gcagtcggcg acttgcgcac ggccacgcag
     3181 gcgctcgagg agctgtccgg cgcgcaatgg gcggcgccgc tgcctgcggt ggtcgcgatc
     3241 ccggccgaca gcgacggctg gtccgtgacg ctgcggctgg cggcatcgca ggacagagtc
     3301 cgctggcgcc tgcgcgaaga acgcggcgcc gtccacgagg gcgaggcggc cgtgcaggac
     3361 atggcggagc tgtcgcgcag cggcgccgag ggcggttatc gcagtgaacg ccggctcacg
     3421 atggcgcttc gcctcgggcc gggctaccac caattcagca tcgaggaccc cccgggtgaa
     3481 accctggtgg tgagcgcgcc ccagcagtgc tggcgcccag ccgcgctgcg tgaggaaggc
     3541 aagatctggg gacccgccgt ccagctctac gccctgcgct cgcggcgcaa ctggggaatc
     3601 ggcgacttca gcgacctgtc ggccctggtg gccgaggcgg ctggccgggg cgcgggcatc
     3661 gtgggcctga acccgctgca cgcgctgttc ccccacaacc cggcgcacgc gagcccctac
     3721 agtccgtcgt cgcgccggca cctcaatgtg ctgtatatcg acgtcgaggc cgtctccgat
     3781 ttcgccgtct gcgaaccggc ccgccggctg gtgcggtctc ccgagttcca ggcgcgcctg
     3841 gcgcggctgc gcgaggcgcc gctggtcgac tacgcgggcg tcgccgaggc gaagttcgag
     3901 gtgctggagc tgctgtacgc atgtttcgcc gaacggcatc tgaacccgga cggaagcgcc
     3961 gccgacgaca cgggcgccga gtttgcgtcg ttcgtcgcgc aacgcggcga ggctttgttc
     4021 aggcacgcgc tgttcgaggc gctgcaggcc cactttcatg ccgccgacca ggccgtgtgg
     4081 ggctggcagg tctggcccga gcccttccgc gaccctgccg gcacggaagt cgccgcgttc
     4141 gccgtccggc acgccgggcg ggtgcgcttc caccagtacc ttcaatggca ggcggcgctg
     4201 cagctggcgc gggccgcaca ccacgcggag gtgctgggga tgggcgtggg cctgtacctg
     4261 gacctggccg tttcggtcga tcgcgccggc tccggcgcat ggagcgcgcg cgattgcttt
     4321 gccgagcagg caggtgtggg tgcgccgccg gacgagttca acctgcacgg gcagggctgg
     4381 ggcctgccgc ccctgcggcc ggatcgcttg cgcgccacgc gctatcgcct gttcgtggag
     4441 acactgcgcg ccaacatggt gggcgccggc gccttgcgca tcgaccatgt gatggggctg
     4501 atgcgcctgt tctggattcc ggccggacac ccgcccgcgg acgggaccta cgtgcactat
     4561 cgcctgcagg agctgctgtc gatcgttgca ctggagagcc agcgcaaccg ctgcatggtg
     4621 atcggcgagg accttggcac cgtggccgac gaggtgcgca ccgcgttggc aagcgccggt
     4681 gtgctgtcgt accggctgct gtatttcgag cgcggggccg gcggggattt caagtcgccg
     4741 gccgactacc cgcgcgacgc actggtcgcg gtcggcacgc atgacctgcc gacgctgccc
     4801 ggttggtggt gcgggcacga cctgcagttg cggcaacggc tgggcctgtt ccccagccag
     4861 ccatcgttcg aacagcagtt gctcgagcgt gcgcaggacc gcgtgaagct gctgctggcg
     4921 gtgcagcgcg ccggcctgct gacgcccgag gacgtggcgg ctgccgccgg cgcgcaggcg
     4981 ccggcgaccg gtggggtcga agcgatccat gcgttcctgg cgcaggcccc gtcggcggtg
     5041 atgatggtcc agctggagga tgtgctgggc cagacggagc aggccaacct gccgggaacc
     5101 acgaacgagc atcccaactg gcaacgtaaa ttgccgctgg aactggaagc gctgcccacg
     5161 gaagtgcgct ggctgggcct gggccgcagg ttggccacgc tgcggcccag gcgtgcgccg
     5221 cagccggtcg aaccgggggg cacgagcgcc agggtgcctc gcgccaccta ccggctgcaa
     5281 ctgcaccggg aattcggctt cgacgacgcg atccgaatcc tgccgtacct ggaccggctc
     5341 ggcgtgagcc atgtctactg ctcgccggta cagcgcgcgc gcgccggcag cacgcatggc
     5401 tacgacgtgg tggcccacga cgagatcaat cccgaactgg gcggggcgca gggcctgcgg
     5461 cgcttcatcg acgccttgcg cgagcggggc atgggcctgt tgctcgacat ggtgcccaac
     5521 cacatggggg tgctgggcga cgacaacgcc tggtggatgg acgtgctgga gaacgggccg
     5581 gcctcggttt acgcccagca cttcgacatc gactggcagc ccctgaaccg cgagctggaa
     5641 ggcaaagtgc tgttgccgct gctgggcgac cattacggcg atgcactcac ccgtggcgag
     5701 atcgtcctgc agttcgagcc ggccggcggc accctggtgc tgcgctattt cgaacaccgc
     5761 tttccgttgg cacccgatac ctatccgtgg gtgctggacc gggcccaggc gcggctgggc
     5821 gacaccgacc tgtcggccgg cctggccagc atcgcgacgg cgtttggcca tctgccgggg
     5881 cgcgcggccg acagcgcgca ggcgcgcgcc gagcgtgacc gtgacaagga gctgctgaag
     5941 gcgcggctgg cgcggctggc ggcgcggcag cccgccgtcg cccaggcgat cgccgccagc
     6001 gtggcgcagc tcaatctcgc cagtgcccgc gacgagctgc acgcgctgat cgaggcgcag
     6061 gcctaccgcc tcgcctactg gcgcgtagcc gccgacgaaa tcaactaccg ccgttttttc
     6121 gatatcagcg agctcgccgc gctgcgcatc gagcgggacg acgtattcga gaccacgcag
     6181 tccttcgcgc tggaccttgc cgccggaggc gcggtggacg ggctgcgcat cgaccacccg
     6241 gacggcctga acgacccggc ccgctacttc gcgaagctgc aggcggggta cgcggcccgc
     6301 gcggggctgg tgctgcccga gcgggacagc cagggccgcc cctcccggcc gctgtacgtg
     6361 gtcgcggaaa agatcgcggc ggggcacgag gaagttcccg aaagctggca cgtgcacggc
     6421 accacggggt accgcttcgc caatgtggcc aacggcgtgc tggtggacac ggcggcggcg
     6481 gcccggttcg tccagatctg gcacgacttc accgggcagc ggcagacttt cgcggaggtg
     6541 gcgcgcgtga gcaagcgcga catcatgcgc accgcgctcg cctccgaact gaacgtgctt
     6601 tcggccgaac tgctgcgcat cgcgcgggcc gaccggcgca cccgcgacta cacgctgaac
     6661 gcgctgcggc gcgcgctggc cgacgtggtg gcctgcctgc cggtgtaccg gacctacatc
     6721 atcgatgcgc cctcgcagca ggacctgcac tatgtgaact gggcggtgaa ggaggctgaa
     6781 cgccacagcg aggacgccga cctgtcggtg ttcgcattcc tgcgccagac cctgctcggg
     6841 cagtgcgtgc ccgatgcgcc ggcggagctg tcggcgcgcg tgcggcgctt cgcgaccagg
     6901 cttcagcagt tcagcgcccc ggtcgcggca aaaggggtgg aggacacggc cttctaccgc
     6961 tactttccct tgagttcgct caacgaggtg ggcggcgagc cggcgcagtt cggcatgacc
     7021 gtgccgcagt tccacgaagc cagcgccgat cgctgccggc gctggccgca cacgatgctg
     7081 gcgacctcca cccacgatca caagcgttcc gaggacgtgc gcaatcgaat caacgtgctg
     7141 tcggagatgc ccgcggcctg gcggctggcg ctgcggcgct ggaggagcct gaatcgcggc
     7201 attcgcgacc ggctggtggc cgcgggcgct cccgcggacg caccgtcggc ggccgacgag
     7261 tacctgctct accagacctt gctcggcacg ctgccgccgg agcccgccga tgcgccgttg
     7321 cggcaagcct ggcgcgaccg ggtcgtgcgc tacatgctca aggccgcgcg agaggccaag
     7381 ctgcataccc ggtggacaca tccggacgaa gcctacgaag cagccctgga aggcttcgtg
     7441 gacggactgc tctcgcccgc gggcggcgcc agcgcgttcc tgacggacct gcatgcgctg
     7501 ggtgcccgcg tggcctggtt cggggccctc aacagcctta gcacgacctt gctcaagcac
     7561 acctcgcccg gtgtgccgga cctgtaccag ggccacgagg tgatcaacct gacgctggtg
     7621 gacccggaca acaggcgccc cgtcgactac gagcggctcg ggcaggcact ggcagacttg
     7681 caggcgatgg atcctggcgc cctcgcggcg ctcgcggctg caccagccaa cggcctggcc
     7741 aagctgtgga tcgcctggcg cctgctccgt ctgcgtcggc agcagcccga cctgttccgg
     7801 agcggcgact accaggccct ggaaaccggc ggccggcact ccgggcatgt gctggcttac
     7861 gcgcggcgga acgagggcat gacgctggtg gtcatcgcgg gccggctttg gtccggtttg
     7921 cccgccgaag ccggcaccgg gcctctggga gaggccgtgt ggtccgatac gtgggtggcc
     7981 gtcggcctgc cgcacgggac tgcgctcacc gacgtgctca gcggccgtga tctgaccgtc
     8041 gaaaacgggc gcatcgcagt gggccgggcc ttctcgtgcc tgcccgcggc ggcgctggtc
     8101 tgcctggcct aaaggcactt ccatggcgga cggcagcccc ttcaagatcc ccgaatccgt
     8161 gctggtggtg atttacaccc cggcgctcga tgtgctattg gtcgaccgca ccgatgcccc
     8221 gggtttctgg caatcggtga ccggcagcaa ggacagcgcg aacgaaaccc tcgcgcagac
     8281 cgccgtacgc gaggtgttcg aggaaaccgg catcaactgc ggcgtcggca ccaccttgca
     8341 cgcgggcctc aaggactggg gcctggagaa tatctacgac atctacccgc gctggctgca
     8401 ccgctatgcg cccggcgtcg tacgcaatac cgagcacgtg ttcggcctgt gcgtgccgcc
     8461 cggcacgcct gtcacgctga gcccccgtga gcacacggga taccgctggt taccttggcg
     8521 ggaggcggcc gacgcctgct tctcgccttc caacgccgag gcgattctgt tgctgccaca
     8581 atttgccgga tgagcgcagc gcgcaatggt ttcccgcaag ccgggtcttg cacacccggc
     8641 ggcggcggct cggggagcgc ctgatgccag tgctgcgcgt cgcgacttac aacatccaca
     8701 aaggggtaca gggcgtcgga cccgggcgac gcctcgaaat ccacaacatc gggcatgcgg
     8761 tcgagcaact cgacgcggat atcgtctgcc tgcaggaagt gcgcaagctc caccgccgcg
     8821 agcagcggta tttcacccgc tggccggaat tgccgcaggc cgagttcctt gcgcccgagg
     8881 gctacgcttc ggtgtaccag accaacgcga ccacccgcca cggcgaacac ggaaatgccc
     8941 tgttgtcgcg ctggcccgtg gtgtctcatg gccacgagga catgtcggac caccgcttcg
     9001 aacagcgcgg attgctgcac gttgaagtcc atgcctaccg ccgacccatc catgtgatcg
     9061 tgctgcatct gggcctgatc gccggcagcc ggctgcggca gatcgagcag ctcggccttt
     9121 acatccagcg cgaagtgccg cggcgcgagg cgcttgtcgt ggccggcgac ttcaacgact
     9181 ggggcggaaa gctgcgttcg gcgatggatg ggtacaggct gaaggatttc gtcggcgaaa
     9241 ggctgctgac ctaccccgcg cggctgccgc tcgcgcaact ggactacatc tacgcgcgcg
     9301 gcctcaagcc gctgggcgtg caaataccca gaggccggat ctggtggcgg atgtccgacc
     9361 atctgccgct gatcggggag ttcaaggtct agggacatgt cgcatgcttc ttccgtgccg
     9421 cgcggcggcc atgacctgca actgctgcaa ggcagcgggg agctgtttcc cgcgctggtg
     9481 gaggcgatcg attcggcccg gcgcgaagtg cggctggaga cctatatctt cgacttcacc
     9541 gggcacagcg ccgagatcgc ctacgcgctg gagcgggcgg cgaggcgggc ggtgagtgtc
     9601 aaggtcgtgg tcgacgggtt cggcagcgcc cccctgccgc cggcgtgggc ggaacggttc
     9661 gcgcaagccg gcgtggaatg gcgtgtgtac gcgccgctcg gctggctcgg catcctctgg
     9721 gtcggcaact ggcgccggct gcaccgcaag ctgtgcgtgg tggatggcca gattgccttc
     9781 tgcggcggca tcaacatcct ggacgatttc catgacccga accacgggcc gctggagtct
     9841 ccccggctgg atttttcggt tcgggtgacc ggcccgctgg tggccgaggt tcacgcggcc
     9901 atgacacggc tgtggctgcg catgcaggcc atgagccagg cgcgccgggc acgcctggcc
     9961 gatatgctgg gttcgctgcg tgaatacggg cttggccggg cccgccggcc cgcgccggaa
    10021 cccgggcagc ggagatcgcg ggccgcgctg gtgctgcgcg acaacctgcg caatcgcgcg
    10081 cgcatcgagc gcgcctaccg ccgcgccatc ggccgggcgc gcgaggaaat catcatcgcc
    10141 aacgcttatt tcgtgcccgg acgcaagatg cgcaaggccc tggtgtcggc tgcgaaccgc
    10201 ggcgtgaagg tccggctgct gctgcagggc cgttacgagt acttcatgca gtactacgcc
    10261 gcgcggccga tctatggcgc gctgctgcgc gccggcgtgg aaatctacga gtactcgccc
    10321 agcttcctgc acgccaaggt ggcggtgatc gatggccgct gggccacggt gggatcgtcc
    10381 aacctggacc cgctgagcct gctgctggcg cgcgaggcca acgtgatcgt cgaagacgag
    10441 gacttcgcgg cccggttgcg ccgccacctg ctgcgtgcca ttcgcgcgga ggaccaggcg
    10501 atggaccccg agggctttcg ccagcgcccg ctgcgccagc gcttcatgga aggcgtggcg
    10561 ctcgcgctca tgcggctggc gctggcgatc cagggcaaga agtatcttta atacattggg
    10621 ggacagatca agaattgctt cagcaatttt gctctgtcct caagtgatta agctttgcgg
    10681 gacagatctt taactctgtc cccaactgaa taagcttgtg ggacagatct tcagctctg
//
LOCUS       DASWIY010000470         5679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3295723, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000470 DASWIY010000000
VERSION     DASWIY010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3295723"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1306)
                     /gene="urtA"
                     /locus_tag="VGA59_16815"
     CDS             complement(53..1306)
                     /gene="urtA"
                     /locus_tag="VGA59_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEX9721382.1"
                     /translation="MQRRDFLKFSAGLAGAATLPASMQAFGAKDPIKVGVLHSLSGTM
                     AISETALKNTALMTFEQINAAGGIMGRPIEPVVVDPASNWPLFAEKARQLITQEKVAA
                     VFGCWTSVSRKSVLPVFEELNGLLFYPVQYEGEELSQNVYYTGAAPNQQAIPAVEYLM
                     SEDGGSAKRWFLLGTDYVYPRTTNKILRAFLKSKGVAEKDIEEIYTPFGHSDYQTIVA
                     NIKKFSAGGKTAVVSTINGDSNVPFYKELGNQGLKATDVPVVAFSVGEEELRGIDTKP
                     LVGHLAAWNYFMSLKNPQNTQFIDMYKGWAKKNGVPNLDTVVTNDPMEATYVGIHMWK
                     QAVEKAKSIEVDKVRPAMAGQTFKAPCGFTLTMDKTNHHLHKPVYIGEVTATGQFNVV
                     WKTKGPIRAQPWSPFIAGNEGKQGL"
     gene            1678..2286
                     /gene="lexA"
                     /locus_tag="VGA59_16820"
     CDS             1678..2286
                     /gene="lexA"
                     /locus_tag="VGA59_16820"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX9721383.1"
                     /translation="MDELTARQAEVLQLIADFLQATGFPPTRAEIAQQLGFRSANAAE
                     EHLRALERKGYIEMLPGASRGIRLRNTYNVGLPVVGRVAAGSPILSEQHVQGRYQIDP
                     SLFKPRADYLLKVRGMSMRDAGILDGDLLAVHHTGEARSGQIVVARLKDEVTVKRFKR
                     QGSQVQLLPENPDFEPILVDTRRDPLVIEGIGVGIIRNGRTL"
     gene            2485..4767
                     /locus_tag="VGA59_16825"
     CDS             2485..4767
                     /locus_tag="VGA59_16825"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX9721384.1"
                     /translation="MLYCAVMVSVGGRTFSQELLDRISAQVRAQPDISRRQLSLRVCE
                     WMGWRNAAGRAQEMSCRKSLLALHRRGLIELPALKQRYGFQQARGPTVCPPLMQVSCT
                     LAELGEVVLLAVNSNELSRVWRGLLDAHHYLKSGPLCGAQLRYLVRSPRYGWLGGLSY
                     SACAWRVECRDEWIGWTQGARERNHPLVVNNSRFLIAPSVRVKCLASWVLARAQARLA
                     DDWQAWYGYRPLLLETYVERGRFAGTCYRAANWQQVGTTRGRGRQGEGASIKDVYVLA
                     LAGDWQGRLCREADGQVRLRAARSRPAPRDWIEAELGGAHMGDARLTARLLEMTGMFY
                     AKPTANIPQACATARATKAAYRFLDNEKVGWQAILQPHYAATEQRVREQPLVLVAQDT
                     TTLNYSTHPHTQGLGPIGTQSEKVRGLMVHDTLAFTPKGVPLGLLDLQCWAREGIGSR
                     DERHSKPIADKESCKWLDSYAAVSAVQQRARNTRLVLMADREADIHEVFAAQANTPHG
                     AQLLIRAERSRNRQVLGDEQHTHASLWRTLEQQKLLGTREILVAPSDKRAARQAILEV
                     RSAPVTLKPPKRSAHLAPVAVWAVLARELNPPPQVEALEWMLLTTVAVHDEHDAFERL
                     DWYARRWGIEVYHRILKSGCRVEARQLEQAHRLLNCLAIDLIVAWRIYHLTALGEQAP
                     EVPCTIYFTDSEWKALCTFVNRTKTPPALPPSLNEAVRLLGQLGGHLGRNSDAHPGSE
                     VLWRGMARLADIESAYDLYH"
     gene            complement(5101..>5679)
                     /locus_tag="VGA59_16830"
     CDS             complement(5101..>5679)
                     /locus_tag="VGA59_16830"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9721385.1"
                     /translation="LRTSGRVVKIGTTSAGTLVVLKLETRDEGSGEPVVTSYSSTIFL
                     RVPLEGAPGTLEAPPELRGEGAITVLPAGGVALPTARRMAHVYTECAGIWNPIHTQRR
                     IAQEKQLPDVILHGTCTWALAGVELIRRCAEGDPLRLKRLAGRFHGMVIPGTTIGLEY
                     GVEPGEPGKVQFTVTNAAGELAIGHGLAEFAA"
BASE COUNT          925 a         1889 c         1879 g          986 t
ORIGIN      
        1 acgggcatcc gccggcgggc cgcgacggcc cgccccgcgc aacgctcccc gcttacagcc
       61 cctgcttgcc ttcgttgccg gcgatgaacg ggctccacgg ctgggcccgg atcgggcctt
      121 tggtcttcca caccacgttg aactgaccgg ttgcggtgac ctcgccgatg tacaccggct
      181 tgtgcaagtg atgattggtc ttgtccatcg tcagcgtgaa gccgcagggc gccttgaagg
      241 tctgcccggc catggccgga cgcaccttgt cgacctcgat cgacttggcc ttctccaccg
      301 cctgcttcca catgtggatg cccacgtagg tggcttccat cggatcgttg gtcaccaccg
      361 tatcgaggtt gggcacgccg ttcttcttcg cccagccttt gtacatgtcg atgaactggg
      421 tgttctgcgg attcttcagc gacatgaagt agttccaggc ggcgaggtgg cccaccagcg
      481 gcttggtgtc gatgccgcgc agttcctctt cgccgaccga gaacgccacc accggcacgt
      541 cggtcgcctt caggccctgg ttgcccagct ccttgtagaa gggcacgttg gagtcgccgt
      601 tgatggtcga caccactgcg gtcttgcccc ctgccgagaa cttcttgatg ttggccacga
      661 tagtctggta gtcgctgtgg ccgaagggcg tatagatttc ctcgatgtcc ttctcggcga
      721 cccccttgga cttgaggaac gcgcgcagga tcttgttggt cgtacgcggg tacacataat
      781 cggtgccgag caggaaccag cgcttggcgc tgccgccgtc ctcgctcatc aggtattcca
      841 ccgctggaat ggcctgctgg ttgggcgcgg cgccggtgta gtagacgttc tgcgacagct
      901 cttcgccctc gtactgcacg gggtagaaca gcaggccgtt cagctcctcg aacaccggca
      961 gcaccgactt gcgcgacacc gaggtccagc agccgaacac cgccgcaacc ttttcctgcg
     1021 tgatgagctg gcgggccttt tcggcgaaca gcggccagtt ggaagccggg tcgaccacca
     1081 ccggctcgat cggacgtccc atgatgccgc cggccgcatt gatctgctcg aacgtcatca
     1141 gggccgtgtt cttcagcgcg gtctcgctga tcgccatggt gcccgacagc gagtgcagca
     1201 ccccgacctt gatgggatcc ttcgccccga acgcttgcat ggacgccggg agcgtagcgg
     1261 cgccggccag accggccgag aatttcagaa agtcgcgtct ttgcatggaa agcttcccct
     1321 gttggtgagt gatgaagtgg tgcgctgtga agccgccgct tggccgaacc atgcacggtc
     1381 ttgatgcatc tcggcgacct cctccccgca cacggatggt gctgtgcatc aaccgtgcct
     1441 tgccggcgtg caccatgctc gcgtcatcgt tttcctcatt cggtcatgag cttagacagt
     1501 cggcgcgaaa ccgaagcaac agggtctgcg caaggccgcg agagaatcat gcaccgcagt
     1561 ggtgctcggc tcgcaagcgg cgcacggctt cagggcgagc ccgttggtcc cggcagcatc
     1621 atctgccttg cgcttcatcc cgatctgtat ataatcacag caactggaga taaacgcatg
     1681 gacgaactca ccgcccgaca ggccgaagtc ctgcagctga tcgccgattt cctgcaggcc
     1741 accggctttc cgcccacccg cgccgagatc gcccagcagc tcggcttccg ctccgccaac
     1801 gcggcggagg agcatctgcg ggccctggag cgcaagggct acatcgagat gctgcccggc
     1861 gcctcgcgcg gcattcgcct gcgcaacacc tacaacgtgg gcctgccggt ggtgggaagg
     1921 gtcgccgccg gcagtccgat cctgtccgag cagcacgtgc agggccgcta ccagatcgac
     1981 ccgtcgctgt tcaagccgcg cgccgactac ttattgaagg tgcgcggcat gagcatgcgc
     2041 gatgccggca tcctcgacgg cgacctgctg gccgtgcatc acaccggcga ggcgcgctcc
     2101 ggccagatcg tcgtcgcgcg gctgaaggac gaggtcaccg tcaagcgctt caagcgccag
     2161 ggcagccagg tgcagctgct gccggaaaac cccgacttcg aaccgatcct ggtggacacc
     2221 aggcgcgatc cgctggtgat cgagggcatc ggcgtgggga tcatccgcaa cggcaggacg
     2281 ctgtaataaa agcaggcgtg ggacgtaagg acgtaggacg tagtaaggcg caaggtgcgt
     2341 ggctcacgca ctgagccaga agattagcca gagttcgcat ggagcgaggc tctctcctcc
     2401 ccctctcggg gccctttgca ggagagctcc ctctcccctt gcgctgggct tcgccctcaa
     2461 gaatgactgg acacaatttc ggccttgctg tactgtgccg tcatggtcag cgtgggcggc
     2521 cgtacatttt cgcaggaact gttggatcgg atcagcgcgc aggtgcgcgc gcagcccgac
     2581 atctcgcggc gccaactgtc gctgcgggtg tgcgagtgga tgggttggcg caacgccgcg
     2641 gggcgagccc aggagatgag ctgccgcaag agcctgctgg cgttgcaccg gcgcgggctg
     2701 atcgagttgc cggcgctcaa gcagcgctat ggcttccagc aggcgcgcgg gcccacggtg
     2761 tgtccgccgc tgatgcaggt ctcgtgcacg ctggccgagt tgggcgaggt ggtgctgctg
     2821 gcggtgaatt cgaacgagct gtcgcgggtg tggcgcgggc tgctcgatgc gcatcactat
     2881 ttgaagagcg gcccgctgtg cggggcgcag ctgcgctacc tggtgcgcag cccccgttac
     2941 ggctggctgg ggggcctgag ctacagcgcg tgcgcctggc gggtggagtg ccgcgacgag
     3001 tggatcggtt ggacgcaggg tgcgcgcgag cgcaaccacc cgctggtggt caacaacagc
     3061 cgctttctga tcgcccccag cgtgcgggtg aagtgtctgg cctcctgggt gctggcgcgc
     3121 gcgcaggcgc gcttggccga tgactggcag gcgtggtacg gctatcgtcc gctgctgctg
     3181 gagacctatg tggagcgggg ccgctttgcg ggcacctgtt accgtgcggc caactggcag
     3241 caggtgggca ccacgcgcgg gcgtgggcgc cagggcgagg gggccagcat caaggacgtg
     3301 tacgtgctgg cgctggcggg ggattggcag gggcggctgt gccgggaagc cgacgggcag
     3361 gtgcgcctgc gcgccgcgcg cagccggcct gccccgcgcg attggatcga agccgaactg
     3421 ggaggcgcac acatgggcga tgcgcgcttg accgcccgct tgctggagat gaccgggatg
     3481 ttctacgcca agcccacggc caacatcccg caggcctgcg ctacggcgcg ggcgaccaag
     3541 gccgcctacc gctttctgga caacgagaag gtgggctggc aagccatttt gcagccccat
     3601 tacgcggcca ccgagcagcg cgtgcgcgag cagcccttgg tgctggtggc gcaagacacc
     3661 accactctga actacagcac ccatccccat acccagggcc tgggtcccat cggcacgcaa
     3721 agcgagaagg tgcgcggtct gatggtgcac gacaccctgg ccttcacccc caagggcgtg
     3781 cccctgggcc tgttggactt gcagtgctgg gcgcgcgagg gcatcggctc gcgcgatgag
     3841 cgccacagca agcccatcgc ggacaaggaa agctgcaaat ggctcgacag ctatgccgcg
     3901 gtgtcggccg tgcagcagcg tgcgcgcaac acccggctgg tgctcatggc cgaccgtgaa
     3961 gccgacatcc acgaggtgtt cgccgcccag gccaacacgc cccacggggc gcagttgttg
     4021 atccgtgccg agcgctcgcg caaccgccaa gtgttgggcg acgagcagca cacccacgcc
     4081 tcgctgtggc gcacgctgga gcagcaaaag ctccttggca cgcgcgagat cctggttgcg
     4141 cccagcgaca agcgcgccgc acgccaggcc atcctggaag tgcgcagcgc ccccgtgacc
     4201 ttgaagcccc ccaagcgcag tgcccacctt gcgcccgtgg ccgtgtgggc ggtgctggcc
     4261 cgcgagctca accccccgcc ccaggtcgaa gccctcgaat ggatgctgct caccaccgtg
     4321 gcggtccacg acgagcacga tgccttcgag cggctggact ggtatgcgcg ccgctggggc
     4381 atcgaggtct atcaccgcat cctcaaaagc ggttgccgag tcgaggcacg ccagctggag
     4441 caagcccacc gcttgctcaa ctgcctggcc atcgatctga tcgtggcctg gcgcatctat
     4501 catctgaccg ccctgggaga acaagctccg gaggtgccgt gcacgatcta tttcaccgat
     4561 tcggaatgga aagccctgtg caccttcgtc aaccgcacca agaccccgcc ggccttgccc
     4621 ccgagcttga acgaagcggt gcgcctgctg gggcagctgg gtggccatct tggacgcaac
     4681 agcgacgctc acccgggcag cgaggtcctg tggcgcggca tggcccgcct ggccgatatc
     4741 gagtcggcat atgacctcta ccattgaacc atcaaacatc cggcatccca tccacctgga
     4801 ctcgccatca ccttgtgctc acgagggatg tgggtaaaga tcagcgcaag gggagaggga
     4861 tctttctgtc tgttgcctgt cccgcgaggg gagagggaga ctcccttgcg cccgccctgc
     4921 gggctgccgt gctcacgcgg gtggggcggt gtggggccgg cgactcctcg gacagactcc
     4981 caccaccccg tcgcgctgcg cacgccaccc ctcctccggc aggaggggaa agcctattca
     5041 tccccgcctc tcgcgacacg gggcccgccg agcggcggtg tagggttgcc ggcttccggg
     5101 tcacgcggcg aattccgcga gaccatgacc gatggccagt tctcctgcag cattggtcac
     5161 ggtgaactgc accttgccgg gctccccggg ctcgacgcca tattcgagac cgatggtggt
     5221 gcccgggatc accatgccgt ggaagcgtcc cgccaggcgc ttcagccgca gcggatcgcc
     5281 ctcggcgcag cggcggatca gttccacacc ggccagcgcc caagtgcagg tcccatgcag
     5341 gatcacatcc ggcagctgtt tctcttgcgc gatgcgccgc tgcgtgtgga tcgggttcca
     5401 gataccggcg cactcggtgt agacgtgcgc catccgccgg gctgtgggaa gcgccacgcc
     5461 gccggcggga aggaccgtga tggcaccctc accccgcaac tccgggggcg cctcgagcgt
     5521 ccctggcgcg ccctccagcg ggacacgcag gaagatggtg gaggaatagc tcgtcacgac
     5581 gggttcgccg ctgccctcgt cccgtgtctc gagcttgagg accaccagtg tgccggcgct
     5641 ggtcgttccg atcttgacga cccggccgct ggtgcgcag
//
LOCUS       DASWIY010000471        11252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2933648, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000471 DASWIY010000000
VERSION     DASWIY010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2933648"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VGA59_16835"
     CDS             <1..206
                     /locus_tag="VGA59_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721386.1"
                     /translation="QLGRVMRLLAELGIELKADVPQDVVPVLAALKLTGVKPLKPRRH
                     LPNPGDADRPDAKKNSRPPEDKR"
     gene            203..1585
                     /locus_tag="VGA59_16840"
     CDS             203..1585
                     /locus_tag="VGA59_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEX9721387.1"
                     /translation="MTARQLEVLVSDRLIGHLREENDLWQFEYDSAWRESPQAFDLSP
                     ALSRGQALHADGATNRPVQWYFDNLLPEDSLREVIAKEADLIAEDAFGLLAYFGAESA
                     GSLVLRNPARSLLAEHGLKPLSLEVLNQRILNLPNASLTKDAPKRMSLAGAQHKMLVV
                     LQGGELFEPLPATPSTHILKPNHQSSDYSASVMNEFFTMRLARAVGLEVPAVHRLYAP
                     QPVYIVERFDRVRVSDGGAAPDAVQRRHVIDTCQLLNKARTFKYTAASLQTLADAIQH
                     CRYKAATRLRLYQWVVFNVLVGNGDNHIKNISFTVDSEGINLAPAYDLLCTAVYETTA
                     LANEKAHWPRSQLALSLNDAATFADVTPDHIFKAAGILGLNRSTAARELDRLLRTVLP
                     EANKLVAEIEASIAEDAAASPDPEAARAHIAGELRLLRAVRHIVLADMHRRLSQRPAY
                     HHAGVQSWTR"
     gene            complement(1555..2343)
                     /locus_tag="VGA59_16845"
     CDS             complement(1555..2343)
                     /locus_tag="VGA59_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9721388.1"
                     /translation="MRTGRVAYGGAIHECTPHGSDGLRLADGRVLKEQEVVWLPPIEA
                     RTIFALALNYADHAAELAAKSSAEQSGFLQAQRSEPIVFLKGANTLLGHYGLTRRPAG
                     AEFMHYECELAVVIGREGKRISRQRAMDHVAGYTVANDYAIRNFLEPYYRPNLRVKSR
                     DGCTALGPWLVPRNEVTDPMNLRLTTHVNGTLVQDGNTRDMIHDIAALIEYLSGFMTL
                     SPGDAILTGTPKGSVDVRPGDEVVCEVEAIGRLRNHLVQDWTPA"
     gene            complement(2340..2981)
                     /locus_tag="VGA59_16850"
     CDS             complement(2340..2981)
                     /locus_tag="VGA59_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010118219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9721389.1"
                     /translation="MKTVVGALLNFRGAWAAMEPAMQREPHYKPPVHPVLYIKPANTW
                     RGAGDPIVLPPDVEEIEVGPTLGLVMGRTAARVPVEDALDYVAGYVIVNDVTVPHTSV
                     LRPPLKQKCRDSFCPMSTVMPAAEVSDPDSLGIRVYVNGELRLENSTVNLVRNVSKLI
                     ADVSEFMSLARGDVLLVGVPESAPRARAGDRVTVEIDGLGRLENTLRAEGATA"
     gene            complement(2978..3865)
                     /locus_tag="VGA59_16855"
     CDS             complement(2978..3865)
                     /locus_tag="VGA59_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9721390.1"
                     /translation="MADYHPFHPNPRVPARMPPPGTIDSQFHVLGPADKYPVRPGAAY
                     EMPSATWEAARRVHQTLGISRGIVVQTTTYGADHSVVLDGLAALGPGYKGCANALVFE
                     DADDAYLEKLHAAGVRGARFSFRQALGAVLSREAFDRAVGKLRELGWYMKIQPEQSGI
                     VESAHWFEHLDLPILIDHMGRADAVLGPDRNPNIAKLRQLLARGNFWVMLSLGEKISK
                     AGAPYDDVVPIARALIDAAPDRCVWASDWPHPVSKQPPPNDADLLELMYRYVRDEAEL
                     RKIMVDNPSQLFGYENTAP"
     gene            complement(3867..4277)
                     /locus_tag="VGA59_16860"
     CDS             complement(3867..4277)
                     /locus_tag="VGA59_16860"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9721391.1"
                     /translation="MAKRQTLYVEGFGHANPIPAACRKGPLLMTGLVNGTDPATGKLA
                     VTLEEQCGHMFTQVRRILEAGGGSTDDIVRMAVWLKDRSQRGPLNEQWLAMFPNPSDR
                     PARLSLQAGELSGGILVQCEVTAYIDSTLSRQDI"
     gene            complement(4286..5227)
                     /locus_tag="VGA59_16865"
     CDS             complement(4286..5227)
                     /locus_tag="VGA59_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9721392.1"
                     /translation="MALATALAGPVTAQDWPSKPVKLVLPYPPGGNVDNAARIVAAEL
                     EKELKQTVIVENKAGAGGMIAGEFVAKAEPDGYTLFVAANGPLLYSPTIFNRPLYHWK
                     SSFAPISSISFTPLVLQVHPSVPAKTAAELIALAKAKPNSLNMASPGAGTTNHLLSEM
                     LQRVSGAKWTTIHYKGNAPATNDLLGGQVQFNLDQVSVAQAFIKDGRTRALAVVAPKR
                     VPWLPDVPTMAEQGIKGVEGLTFTGVLAPAGTPPTVIARLSAALRKVLEQTTVKAKFF
                     AAGAEARWMAPRDFSSYLAKEEATWMPIIKSANITAQ"
     gene            5446..6084
                     /locus_tag="VGA59_16870"
     CDS             5446..6084
                     /locus_tag="VGA59_16870"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9721393.1"
                     /translation="MRILAADLEPQQTYRLITGIVVPRPIAWVCTCSPEGVLNLAPFS
                     AFTFVSNKPPMLGINVGRKAGVRKDTGRNIHETGEYVVHIGDESMVDAIHESAVEHPP
                     EVSEIELLGLRTTPSDFISVPRLVAPPIAMECRLHQAIAFGATGSEFIVGEVLAFHIR
                     DGLLRDGKIDSTELNPVCRLAGPNYATLGRVISKRPLVQTDKTVMSGAEDRG"
     gene            6081..6917
                     /locus_tag="VGA59_16875"
     CDS             6081..6917
                     /locus_tag="VGA59_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9721394.1"
                     /translation="MKKPRAAAGGAEPTDGAQSVRRALAVLRLVASGQERGVRLTDIA
                     VMSGLSRPTVHRLLKVLIDETAVEQDPATKHYLVGTEMSLLGLARGGRFPLRAVAEPY
                     LQALAAEVGDTVFLSVRHGADSVCVARYLGSHPIQVLSIDVGVRRPLGGSVSGIVLLA
                     AMNQDESRELTQTNASRLALQKRDTSAILREVRAARDQGHAYAAQGVMPGTSAVAVPI
                     RDNRGHVLAAVSVAALADRLDRKRLPDVLARIKVQTERITKRHTEIAKHAARRNSPGS
                     GA"
     gene            6880..7683
                     /gene="hpaH"
                     /locus_tag="VGA59_16880"
     CDS             6880..7683
                     /gene="hpaH"
                     /locus_tag="VGA59_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HEX9721395.1"
                     /translation="MLQEEIHQALARELHAAERTRVQVGHFSRRYPDMGIDDSYAVQR
                     AWMAIKRAEGRTVIGHKIGLTSRAMQLASQIAEPDYGTLLDDMLIHDGAEVDAARFIV
                     PRFEVEFAFILSRPLEGPGIMLFDVLRATDYVVPALELIDARIEQFDRDTRAPRKVID
                     TIADNAANGAIVLGGRPMKPDAVDWRWAGALLFKNGVIEESGLGAAVLNHPGNGVAWL
                     ANKLAPHGEGLAAGEIVLGGSFTRPVACAAGDVFHADYGPLGSISVRFV"
     gene            7691..8500
                     /gene="hpaI"
                     /locus_tag="VGA59_16885"
     CDS             7691..8500
                     /gene="hpaI"
                     /locus_tag="VGA59_16885"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HEX9721396.1"
                     /translation="MKTPENEFKKALAGDQLQIGLWLALADSYPAELCAGCGFDWLLI
                     DGEHAPNDLRTMLAALQAIAPYPTHPVVRIPKGDDTLIKQVLDLGAATVLVPMVETAA
                     QAAQLARAMRYPPQGIRGVGSSIARSGRWSHYSDYLHTANDKVCLLVQVETQQAMAQI
                     EEIAAVPGVDGVFIGPADLAASMGFLGKAEHPQVKEAIDTGIAAIVRAGKAPGILCAD
                     EALARHYIAAGVRFVAVGVDTSLLVRAASGLAARFKAPSTGIAAAPASPTY"
     gene            complement(8999..9445)
                     /locus_tag="VGA59_16890"
     CDS             complement(8999..9445)
                     /locus_tag="VGA59_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX9721397.1"
                     /translation="MRVRTVLVLLAVMLLAVFAAINWPVFVAPAQLSLLVTSFTAPFG
                     LLMLGLVIVLVLAFAAYMAAWQGQILMESRRHGKELELQRALADQAEASRFTELRGLL
                     HTEVEQLSAHLAQVQEVLRTDIRENANSLAANLAEMDDRLRLGRSA"
     gene            complement(9574..10686)
                     /locus_tag="VGA59_16895"
     CDS             complement(9574..10686)
                     /locus_tag="VGA59_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX9721398.1"
                     /translation="MQDYIAWLASYDIAGISLLNITLALAAAVASYALMLLGLRLVTG
                     RLRKLAERTTTPMDDVAVTVLASTNRWLLLAAALLIGVGLLDLGPRWSDRMGQMWFVA
                     LTLQVGLWVTRGIGVGVRGYEARNGRSGSQLSASGTLLSWLLRTVLWAVILLSVLANM
                     GVNITAMVASLGVGGIAVALAVQNILGDLFASLSIAVDKPFEVGDFISSDAGIGTVEY
                     VGLKTTRIRALSGEQIVVGNTDLLKQPVKNYRRMRERRIAFKFGVTYDTSPDLAEAIP
                     GIVQQLVEADGKLRFDRAHFQSFGESSLDYEVVYYVLDPAYNLYMDAQQRLNLGLLRE
                     FAARGIEFAFPTRTLYLANPASPGEVPAAREAVAGA"
BASE COUNT         2050 a         3680 c         3531 g         1991 t
ORIGIN      
        1 tccagctcgg ccgcgtcatg aggctgctgg cagaactggg tatcgagctt aaggcagacg
       61 ttccgcagga cgttgtgccc gtcctcgctg cgctcaagct aaccggcgtc aaaccactga
      121 agcctcgccg ccacttgccg aacccaggcg atgccgaccg ccctgacgcg aaaaagaatt
      181 cgcgcccgcc ggaagacaag agatgaccgc acgccaactt gaagtcctcg tcagcgaccg
      241 attgatcggc catcttcgcg aagagaacga tctttggcaa tttgaatacg actcggcctg
      301 gcgcgaatcc ccgcaagcgt tcgacctgtc accagccctg tcgcgcggcc aggcactgca
      361 tgccgacggc gccacgaatc ggcccgtcca gtggtacttc gacaacctgc tgccggaaga
      421 ttctctgcgt gaggtcatcg ccaaagaggc cgacctgatc gccgaggacg cgttcggcct
      481 gctggcttac ttcggcgccg agtccgcggg ttccctggtg ttgcgaaacc ctgccaggtc
      541 gctccttgcg gaacatgggc tcaagccgct ttcacttgag gtgctgaatc agcgaatcct
      601 caatctgccc aacgcgtccc tcacgaagga cgccccgaag cggatgtctc tggcgggagc
      661 acagcacaag atgctcgtgg tcctgcaggg cggcgagctg ttcgagccgc tgccggcgac
      721 gccctcgacg cacatcctca agccgaatca tcaaagcagt gactactcgg catcggtgat
      781 gaacgagttc ttcaccatgc ggttggcaag agctgttggc ctggaggtcc cggccgtgca
      841 tcggctctac gcgccgcagc cggtctacat cgtggagcgc ttcgatcgtg tgcgagtctc
      901 cgacggtggg gcggctcctg atgcggtgca gcgtcgccac gtgatcgaca cctgccagtt
      961 gctgaacaag gcgaggacgt tcaaatatac ggccgcgagc ctgcaaaccc tcgccgatgc
     1021 catccagcat tgccgctaca aggcggcgac tcggctacgg ctttaccagt gggtggtgtt
     1081 caacgtcctg gtcggcaatg gcgacaatca catcaagaac atttccttca cggtcgactc
     1141 cgaaggcatc aacctggcgc ccgcatacga cttgctgtgc accgcggtgt acgagaccac
     1201 ggcgctcgcc aacgagaagg cccattggcc gcgaagtcag ctggctcttt cattgaacga
     1261 tgcggcaacc ttcgcggacg tgacgcccga ccacatcttc aaggccgcgg ggatcctcgg
     1321 tttgaatcgg agcacagcag cgcgcgagct ggatcgcctg ctgcgcacgg tactgcctga
     1381 agcgaacaaa ctcgtcgcag aaatcgaagc gagcatcgct gaagatgccg cggcaagtcc
     1441 ggacccggaa gcggcaagag cccacatcgc gggtgagctt cgcctgcttc gtgctgtcag
     1501 gcacattgtt ctagcggata tgcacaggcg cctctctcaa cggcccgcgt accatcacgc
     1561 cggcgtccag tcctggacca ggtgattccg cagccgcccg atcgcctcga cttcgcagac
     1621 tacctcgtcc ccaggccgca cgtcgaccga tccctttggc gtccccgtca ggatcgcgtc
     1681 gccagggctc agcgtcatga acccgctgag atattcgatc agcgcggcga tgtcgtgaat
     1741 catgtcgcgc gtgttcccat cctgcaccag cgttccgttc acatgcgtgg tgagccgcag
     1801 gttcatgggg tccgtgacct cgttgcgcgg gaccaaccac gggccgagcg cggtgcagcc
     1861 gtcacggctc ttcacccgca ggttgggccg gtagtacggc tccaggaagt tccggatcgc
     1921 gtagtcgttg gccaccgtat agcccgcaac atggtccatc gcccgctgac gggagatccg
     1981 ctttccctcg cgaccgatga cgaccgccag ttcgcattcg tagtgcatga actccgcccc
     2041 ggccgggcgg cgggtcaagc cgtagtggcc aaggagcgtg ttcgctcctt tgagaaagac
     2101 gatcggttcg gagcgctgcg cctgcaggaa gccggactgc tccgccgaag actttgccgc
     2161 gagctcggcg gcgtgatcgg catagttcaa tgccaacgcg aagatcgttc gcgcctcgat
     2221 gggcggcagc cagacgacct cctgctcctt gagcacgcgc ccatccgcca gccggaggcc
     2281 gtcgctgcca tgcggcgtgc attcgtggat ggctccgcca taggcgacac ggccagttct
     2341 catgcggtgg cgccttcagc ccgcaaagtg ttctcgaggc gccccagccc gtcgatttcg
     2401 acggtgacgc ggtcacccgc ccgagcccgt ggggcgctct cgggtacccc aacaagaaga
     2461 acgtcacccc gggcgagcga catgaactcg ctgacgtcgg caatcagctt gctaacattg
     2521 cgcaccagat tgaccgtgct gttctccagc cgcagctcgc cattgacata gacgcgaatt
     2581 cctagcgaat ccgggtcgga aacttccgcg gcaggcatca ccgtgctcat cgggcaaaag
     2641 ctatcccggc acttctgctt caagggtgga cgaagcacgc tcgtgtgcgg cactgtcacg
     2701 tcattgacga tgacatagcc cgccacgtaa tccaatgcat cctcgacggg tacgcgcgcg
     2761 gcggtgcggc ccataacaag gcccagcgtg gggccgacct cgatctcctc gacatcgggc
     2821 ggcagcacga tgggatcgcc cgcgccgcgc cacgtgttcg caggcttgat gtacagcacc
     2881 gggtgcaccg gcggcttgta atgcggctcg cgctgcattg ctggctccat ggccgcccag
     2941 gcgccgcgaa agttgagcag cgctccaacc acagtcttca tggggcagtg ttctcgtaac
     3001 cgaacagctg gctcgggttg tccaccatga tcttgcgcag ctcggcctca tcccgaacgt
     3061 agcggtacat cagttccagc aggtccgcgt cgttcggcgg cggctgcttc gacacggggt
     3121 gcggccagtc ggaagcccag acacatcggt ccggcgccgc gtcgatcagg gcccgcgcga
     3181 ttggcacgac gtcgtcgtaa ggtgcaccgg ccttggagat cttttcgccc agcgacagca
     3241 tgacccagaa atttccccgg gcgagcagct ggcgcagctt ggcgatgttg gggttgcggt
     3301 ccggacccag cacggcgtcc gcgcggccca tgtggtcgat gaggatgggt aggtccaggt
     3361 gctcgaacca gtgcgcgctt tcgacgatgc cgctttgctc cggctgaatc ttcatgtacc
     3421 agccgagctc tcgcaacttg ccgaccgcac ggtcgaatgc ctcgcgcgag aggaccgcgc
     3481 ccagcgcctg gcggaacgaa aagcgcgcgc cccgcactcc ggccgcgtgc agcttctcca
     3541 ggtaagcgtc atcggcatcc tcgaagacga gcgcgttggc gcagcccttg tagccgggac
     3601 ccagcgcggc cagtccgtcg agcaccacgc tgtggtcggc accataggtc gtggtctgga
     3661 cgacgatgcc gcgagagatg ccaagggttt gatgcacgcg ccgcgcggcc tcccaggtgg
     3721 cgcttggcat ttcgtaggcc gcgccagggc gtaccgggta cttgtccgcg gggccgagca
     3781 catgaaactg gctgtcgatc gtgccaggcg gcggcatccg ggccggcacg cgcggattcg
     3841 ggtggaaagg atggtaatcg gccatgtcag atatcctggc gcgacagggt tgagtcgata
     3901 taggccgtga cctcgcactg caccaggatg ccgcctgaaa gctcgccggc ctgcaggctc
     3961 agtcgcgcag ggcggtcgct cgggttggga aacatcgcca gccactgctc gttcagcggc
     4021 ccgcgctggg agcggtcctt cagccacacg gccatgcgga cgatgtcatc cgtgctgccg
     4081 ccaccggcct cgaggatgcg gcgcacctgg gtgaacatgt gcccgcactg ctcctccagc
     4141 gtgaccgcga gcttgcccgt ggcggggtct gtgccgttga ccagccctgt catgagcaag
     4201 ggccccttgc ggcatgccgc cgggatgggg ttggcatggc cgaacccctc gacgtagagc
     4261 gtctgtcgct ttgccatttt cagtatcatt gcgccgtgat gttcgcggac ttgatgatcg
     4321 gcatccaggt agcctcttcc ttcgccaggt agctggagaa gtcccggggc gccatccagc
     4381 gtgcttcggc gcccgccgcg aagaacttcg ccttgacggt ggtttgctcc agcaccttgc
     4441 gcagcgccgc gctcagtcgt gcgatcaccg tcggcggcgt gccggccggc gccagcacgc
     4501 cggtgaaggt cagcccctcc actcccttga tgccctgctc ggccatcgtg ggcacatcgg
     4561 gcagccacgg cacacgcttg ggcgcaacca ccgccagggc tcgcgtgcgg ccgtccttga
     4621 tgaaagcctg ggccaccgac acctggtcca ggttgaactg gacctggcca cccagcaggt
     4681 cattggtggc tggtgcgttt cccttgtagt ggatcgtcgt ccacttcgcg ccgctcacgc
     4741 gctgcagcat ttcgctgagc agatgattcg tcgtgcccgc gcccggagag gccatgttca
     4801 ggctgttggg cttggctttt gccagggcga tcaactcggc ggctgttttg gccggcacgg
     4861 acggatggac ctggagcacc agcggcgtga aggagatcga gctgatcggc gcgaagctgc
     4921 tcttccagtg atagagcggc cggttgaaga tcgtcggcga atacagcagc ggcccattgg
     4981 cggcaacgaa aagcgtgtag ccatcgggtt ccgccttggc cacgaactcg ccggcgatca
     5041 tcccgcccgc gccggccttg ttctcgacga tcaccgtctg cttcagctcc ttttccagct
     5101 cggcagcaac gatgcgcgcc gcgttgtcga cgttgccgcc cggcgggtac ggcaagacca
     5161 gcttcactgg cttgctgggc caatcctggg cggtcacggg gcccgccaga gcggttgcta
     5221 gcgccatcgc catccacatc catttactca tgcttgtctc ctgtgttgca gaacactgcc
     5281 actgtatgca gagggggagc gcgtggcaac gcaaaagtca aacggaattc cacgttatgg
     5341 acttctcatc cagccgagcc tcgcttcttg aatcagcttg tcttagcatt cgctttgccg
     5401 atccagttga ttgaatatgt ccacccaatg gacgtggaga caagcatgcg catcctggcc
     5461 gcggacctcg agccgcaaca gacctacagg ctcatcacgg gaatcgtggt gccccggccg
     5521 atcgcctggg tctgcacctg ctcgccggag ggtgtcctga acctcgcgcc attcagcgcc
     5581 ttcaccttcg tttcgaacaa gccgcccatg cttggcatca acgtcgggcg caaggccggc
     5641 gtccgcaagg acacgggccg caacatccat gagaccggcg aatacgtcgt ccacattggg
     5701 gacgagagca tggtggacgc catccacgaa agcgccgtcg agcatccgcc cgaggtgagc
     5761 gagatcgagc tgttgggact gcgcaccacc cccagcgact tcatttcggt gccgcggctc
     5821 gtcgccccgc cgatcgccat ggagtgccgg ctccatcagg cgatcgcttt cggggccacc
     5881 ggttccgaat tcatcgtggg cgaggtcctg gctttccata tccgcgatgg gctgttgcgc
     5941 gacggcaaga tcgacagcac cgaactcaac cccgtctgcc ggctggcggg gcccaattac
     6001 gcgacgctgg gacgcgtgat ttccaagcgg ccgctggtcc agaccgacaa gaccgtgatg
     6061 tcgggcgccg aggatcgcgg atgaagaagc cgcgcgccgc ggcaggtggc gccgagccaa
     6121 ccgacggcgc gcaaagcgtg cgccgggccc tggcggtttt gcggctggta gccagcgggc
     6181 aggaacgcgg cgttcgcctg accgatatcg cagtcatgtc gggcctgagc cggcccacgg
     6241 tgcatcgcct gttgaaagtg ctgatcgatg aaaccgcagt cgagcaggac cctgccacca
     6301 agcactatct tgtcggcacc gaaatgtccc tgcttgggct ggccaggggc gggcgcttcc
     6361 cgctgcgggc ggtcgccgag ccttatttgc aggcactggc cgcagaggtg ggcgacaccg
     6421 tgttcctgag cgtgcgccat ggcgccgact cggtgtgtgt cgcgcggtac ttgggaagcc
     6481 accccatcca ggtgctatcc atcgacgtgg gcgtgcggcg tcccttgggt ggcagcgtct
     6541 cggggatcgt cctgctggca gcaatgaacc aggacgaatc acgtgaactc acgcagacga
     6601 atgcatcgcg tctcgcattg caaaaacggg acacgtctgc aatcctgcgg gaagtgcgtg
     6661 ctgcgcggga ccaaggccac gcgtacgccg cgcagggcgt catgcccgga acgagcgccg
     6721 tggccgtgcc gatccgcgat aaccgcggcc atgtgcttgc cgccgtcagc gttgccgccc
     6781 tcgccgatcg gctcgaccgc aagcgccttc ccgacgtgct ggcccgcatc aaggttcaga
     6841 ctgaacgcat caccaaaaga cataccgaga ttgccaaaca tgctgcaaga agaaattcac
     6901 caggctctgg cgcgtgaact gcacgccgcc gagcgcaccc gcgtccaggt cgggcacttt
     6961 tcccgtcgct atccggacat gggcatcgac gacagctacg ccgtccagcg cgcatggatg
     7021 gcaatcaagc gcgcggaagg ccgcaccgtc ataggccaca agatcggctt gacctcacgg
     7081 gcaatgcagc ttgccagcca gatcgcggag cccgactacg gcaccctgct tgacgacatg
     7141 ctcatccatg atggggccga ggtggatgcg gcgcgattca tcgtgccgcg cttcgaggtg
     7201 gagttcgcgt tcatcctgag ccgaccgctc gagggcccgg gcatcatgct gttcgatgtg
     7261 ctgcgggcga ccgactatgt cgttcccgcg ctggaactca tcgatgcgcg catcgaacag
     7321 ttcgaccgcg acacccgcgc gccgcgcaag gtgatcgata ccatcgccga caacgcggcc
     7381 aatggcgcca tcgttcttgg cggccgtccc atgaagccgg atgccgtgga ttggcgttgg
     7441 gccggtgccc tgctgttcaa gaacggcgtg atagaggaat caggcctggg cgcggcggtg
     7501 ctcaatcatc ccgggaatgg cgtggcctgg ctggccaaca agctcgcgcc ccatggcgag
     7561 ggcctggccg ccggcgagat cgtcctgggc gggtctttca cccggcctgt tgcctgtgca
     7621 gccggcgacg tcttccatgc ggactacggc ccgctgggct ccatttcagt caggttcgtg
     7681 tgagcgcccg atgaagacac ccgaaaacga gttcaagaag gcgctggccg gggaccagct
     7741 acagatcggc ctctggctgg cactggccga ttcctatccc gcggagctgt gtgcaggctg
     7801 cggcttcgat tggctgctga tcgacggcga gcatgcaccg aacgatctgc gcaccatgct
     7861 cgccgcgctc caggcgatcg cgccgtatcc aacgcatccc gtggtgcgca tccccaaggg
     7921 cgacgatacc ctgatcaagc aggtgctcga cctgggtgcg gccacggtgc tcgtgccaat
     7981 ggtggaaacc gcagcacagg cagcccagct tgcccgcgcc atgcggtatc caccccaggg
     8041 catccgcggg gtgggcagtt ccatcgcccg ctccggccgc tggtcccact attccgatta
     8101 cctgcacacg gccaacgaca aggtgtgcct gctggtgcag gtggaaacgc agcaggcgat
     8161 ggcgcagatc gaggagatcg cagccgtgcc aggcgtggac ggggtcttca tcggcccggc
     8221 cgatctcgcc gcatccatgg gcttcttggg caaggctgag catccacaag tgaaggaagc
     8281 gattgacacc ggcatcgccg ccatcgttcg cgccgggaag gcgccaggga ttctctgcgc
     8341 agacgaggca ctcgctcgcc actacatcgc agctggagtt cgcttcgtgg ctgttggcgt
     8401 ggataccagc ctcctcgtgc gcgccgcttc gggacttgcc gcccgattca aggcgccttc
     8461 gacgggtatc gctgcggcgc ccgcatcccc tacgtattga agttgcgcgc ggccgagtgc
     8521 ggcctgcagg caggcagctt cattcgcaac tgaaactcgc cgcgctcacc cgccggtcga
     8581 gcgcaatctc gcgttcgcat ccagatgagc cagcagcaag attctccagg ctcatcaaca
     8641 ggacacatct gttccccctt gatggttatt tgtctcaccg agtcgacagg aaatcgctta
     8701 gagtaggctg ctcgaatcgg caacttctcg gagagattga tggcaatacc aacagacacc
     8761 cctgtaccgc aaatgagtga tcaggcaatc gaagccatct tcgctggtgg cggcgtctca
     8821 gatgagtcag cgcaaattca cgttcacgag gaggacatga cccacctgtg catcactctc
     8881 gtcggtaccg ccaccgcgga gggacctact aacggtttcg ttgtgttttg tgaccatggg
     8941 catgggtaac caggaccctt aattgcagcg gcgttttcca gtacttcggc ttggcatgct
     9001 acgctgatcg gccgaggcgc aggcgatcgt ccatttcggc gaggttggct gccagggagt
     9061 tcgcattctc gcgaatgtcg gtgcgcaaga cctcctgtac ttgcgcaagg tgcgctgaca
     9121 gctgctcgac ttcggtgtgt agcaggcctc gcagttccgt gaagcgcgaa gcttcggcct
     9181 ggtcggccag ggctcgctgc agctccagct ccttcccatg ccggcgcgac tccatcagta
     9241 tttgcccctg ccaggcagcc atgtacgcgg caaatgccag caccaggact atcaccaacc
     9301 cgagcatcag caaaccgaaa ggcgcggtga acgacgtcac cagcaggctg agctgggccg
     9361 gagcgacaaa gacgggccag ttgatcgccg caaagaccgc cagcagcata accgccagaa
     9421 ggacaagaac ggtgcgcacg cgcatgagtt tctccttggt gcggaagaag gccgcaccgc
     9481 acggaatcat cgtactccgt gcgtttacgt cgaggcaaat aaccggggga ctgcaagacc
     9541 aactttgaag gaatcgacca tgccggcttc gcctcaagcg ccggccacgg cttcccgagc
     9601 cgcgggaact tcgcccggac tggccgggtt ggccaggtac agcgtgcgtg tcgggaaggc
     9661 aaactcgatg ccgcgggctg cgaattcgcg cagcagtccc aagttgagac gctgctgcgc
     9721 atccatgtac aggttgtagg cagggtccag cacgtagtag accacctcgt agtcgagcga
     9781 actttctccg aacgactgga aatgtgcccg gtcgaagcgc agcttgccgt cggcctcgac
     9841 cagctgttgc acgatgccgg ggatcgcctc tgcgaggtcg ggggaagtgt cgtaggtgac
     9901 cccgaacttg aacgcgatgc ggcgctcgcg catgcgccgg tagtttttca ccggctgctt
     9961 cagcagatcg gtgttgccca cgacgatctg ttctccgctc agagcacgga tccgcgtcgt
    10021 tttcagcccc acatactcga ccgtgccgat gccggcgtcg ctgctgatga agtcgccgac
    10081 ttcgaacggc ttgtcgacag cgatcgagag cgaggcgaac aggtccccga ggatgttctg
    10141 tacggccagc gccacggcga tgccgccgac gcccaggctc gccaccatgg ccgtgatgtt
    10201 gacgcccatg ttggccagca cggaaagcaa gatcaccgcc cacaacaccg tccgcagcaa
    10261 ccacgacagc agcgtacccg aggcgctcag ctgactgccg ctgcggccat tgcgcgcctc
    10321 gtacccgcgc acgccaacac cgatcccccg cgtcacccac agacccacct gcagcgtgag
    10381 cgcgacgaac cacatctgcc ccatccggtc gctccagcga gggccgaggt ccaacaggcc
    10441 gacgccgatc agcaaggctg ccgccagcag gagccaccga ttggtgctgg cgagtacggt
    10501 caccgcgacg tcgtccatcg gcgtggtagt ccgttcggcg agtttgcgta ggcggccggt
    10561 cacgagccgc aagcccagga gcatcagggc gtaagaagcc accgcggccg ccagcgcgag
    10621 cgtgatgttg aggagggaaa ttccggcgat gtcatagctg gcgagccagg cgatgtagtc
    10681 ttgcatgggc tctacgctac cagaacctgc gcggcccgga acggcttgcc cgatccgtcc
    10741 cggcggggcg tcgagcagcg cgagcaagaa agcgaagccg ttgcggggtc cgtgttcgaa
    10801 cgcgcggtta ggctgccccg cctgggcgcc ggtgcgcaag caggttgtag agccagccga
    10861 acacggcgcc cccgattgcg ccgtccacca atccgtacag agttccagta acgatggaac
    10921 ctgcgccagg gccaggatgg tagcccggat agatcgaggc ggcgacgtca aggaaagcgc
    10981 ggccataacc cggccagacc agattcgcca agccaacaac aaggatcgca gcgccccata
    11041 gcagtccgac tgacaaagct agtgcggtga cgttacatct catgatgcct cccttgcgaa
    11101 aagagattta tcgagaaagg tgcatccctc ccgccggcgc aatgaacaca gcgcgaagcc
    11161 cacaagattt tagacctgcg ctttgaccgc agctgcgccg aacgaccctg cagctgttcc
    11221 ggcccggcgc aatgtccggc ttcggttatt tg
//
LOCUS       DASWIY010000472        10557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2557374, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000472 DASWIY010000000
VERSION     DASWIY010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10557
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2557374"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1032)
                     /gene="tolA"
                     /locus_tag="VGA59_16900"
     CDS             complement(103..1032)
                     /gene="tolA"
                     /locus_tag="VGA59_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEX9721399.1"
                     /translation="MSAVIDRLEFAPPPQPGRVRAFALALIAHLLLMLALTWGINWNR
                     ESDNLTAEAELWSSVPRQAAPPEVQSPPPPPPPPPPPEPPAQQVAPPPPPPAPSQADI
                     ALEREKLKKEQALRKQEELERQQKLEARKKREELEREKKLEARKKQEELKKEQLAEKK
                     AAEEKKKKDEQVKAAKAKQQQLEQEKKLAALREDNMRRIQGMAGATGSPTATGTAQRS
                     AGPSNSYAGRIAARIKPNIVFTEIVSGNPAATVQLRVAPDGTIVGRKLIKSSGVKSWD
                     EAVLRAIDKTEVLPRDTDGTVVPEFPVHFRPRD"
     gene            complement(1055..1483)
                     /locus_tag="VGA59_16905"
     CDS             complement(1055..1483)
                     /locus_tag="VGA59_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HEX9721400.1"
                     /translation="MPVMVTSGRARRRTISEINMVPFIDVMLVLLIIFMVTAPLIAPS
                     MIDLPSVAKAPRQPDQVIQVVIGRDESLDLKIDMNTSRLPLEAVVAAVTQAQAGRPAE
                     SSAVVISADRNVKYESVVKVMDKLQQAGVKRVGLSVQLAR"
     gene            complement(1500..2216)
                     /gene="tolQ"
                     /locus_tag="VGA59_16910"
     CDS             complement(1500..2216)
                     /gene="tolQ"
                     /locus_tag="VGA59_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX9721401.1"
                     /translation="MKPDLSILQLVLNASWVVQLVMLLLVAVSIASWAAIFRKLFALK
                     RVNALNDDFERDFWSGISLNDLFAAAAQNAKLAGPMERIFASGMREYQKLRERRVTDV
                     SALLDGARRAMRASYQREVDAVETHLSFLASVGSVSPYLGLFGTVWGIMHAFTGLASL
                     QSVTLATVAPGIAEALVATAIGLFAAIPAVVAYNRFARDIDRVAIKLETFIEEFSNIL
                     HRNLGAQSASASASASASGH"
     gene            complement(2213..2716)
                     /locus_tag="VGA59_16915"
     CDS             complement(2213..2716)
                     /locus_tag="VGA59_16915"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEX9721402.1"
                     /translation="MTHPIHARPVFAFPIRVYWEDTDAGGIVYYANYLKFFERARTEW
                     LRSLGLEQRTLRENTGGLFVVGETSVRFHHPARLDDELLVTACIEEMGRASLIIAQQA
                     FLREGKGRQGTAGALQVGTATSGASAVATSAGALLCEGTIRIGWVEAGNLKPARIPPT
                     ILEALKR"
     gene            complement(2713..3897)
                     /locus_tag="VGA59_16920"
     CDS             complement(2713..3897)
                     /locus_tag="VGA59_16920"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9721403.1"
                     /translation="MRITARARRIEPFYVMEVAKAASQLAREVAHSDRPMIFLNIGEP
                     DFTAPPLVQEAAAKAIRDGLTQYTQATGLDTLRERISDWYARRFGVAVPARRIIVTAG
                     ASAALQLACLALIEAGDEILMPDPSYPCNRHFVSAAEGRAILVPTTAAERYQLSAAKV
                     EAHWNPGTRGVLLASPSNPTGTSIHPHELRRIHEFVSGRGGITLLDEIYLGLSYDETF
                     GHTGLALDDQVVSINSFSKYFNMTGWRLGWMVVPEALVPVIERLAQNLFICPSAVAQH
                     AALACFEAESLAEYERRRAQFKARRDWFIPQLGAMGLAVPVAPDGAFYAWADCSGACE
                     RLGVKDSWDFAFEVMRRAHVAVTPGRDFGSAQTHDFIRFSTANAMAQLHEAAARLAAL
                     MA"
     gene            complement(3936..4469)
                     /gene="nusB"
                     /locus_tag="VGA59_16925"
     CDS             complement(3936..4469)
                     /gene="nusB"
                     /locus_tag="VGA59_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX9721404.1"
                     /translation="MSGDGNPASERAPRQARAGLTSTGARKASAKSARSRAREFALQA
                     LYQYLVGRNEVRAIDLFTRDLAGFHKADSVHYDALLHGCAGEAAQLDALIVPLLDRKL
                     EEISPIEHGVMWIGAYEFLHCPDVPWRVVLNECIELAKEFGGTDGHKYVNAVLNGLAP
                     SLRPAEVEADKASGKAR"
     gene            complement(4466..4930)
                     /gene="ribH"
                     /locus_tag="VGA59_16930"
     CDS             complement(4466..4930)
                     /gene="ribH"
                     /locus_tag="VGA59_16930"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX9721405.1"
                     /translation="MFGAEKGTAANLDGKGLYIGIVQARFNESITNALANACRAELRA
                     LGVADDHVDHVCVPGALEVPIALQGLAEKGGYDALIALGCIIRGETYHFELVANESAA
                     GLNRVSLDYQLPIANVILTTENVEQAVARQTEKGRDGARVAVEMCNLLDHLG"
     gene            complement(4957..6084)
                     /gene="ribBA"
                     /locus_tag="VGA59_16935"
     CDS             complement(4957..6084)
                     /gene="ribBA"
                     /locus_tag="VGA59_16935"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX9721406.1"
                     /translation="MSASSPDLAPVSISPVEDIIADIGAGRIVILVDEEDRENEGDLL
                     LAAEHVTPEAINFMARFGRGLICLTLTRKRCERLRLPPMATRNGDKKGTAFTVSIEAA
                     EGVTTGISAADRARTVQAAVAKNARAEDLVQPGHIFPLQAVEGGVLMRAGHTEAGCDL
                     AAMAGLTPAAVICEIMKDDGTMARLPDLQLFAAQHGLKIGTIADLIEHRSRHESLIEK
                     IGSRPLKTVHGEFMAHAWRDKPSHGLHFALVKGDWSPDETVAVRVHEPMSMLDALEIN
                     RSMHSWNLDASLRRIALEGKGVAVLLNCGEDAQQLLAQFEGKARASHAPERGRLDLRT
                     YGVGAQILRECGVHRMNLMGNPRRMPSMAGYGLEIAGYIAP"
     gene            complement(6081..6749)
                     /locus_tag="VGA59_16940"
     CDS             complement(6081..6749)
                     /locus_tag="VGA59_16940"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX9721407.1"
                     /translation="MFTGIITGVGRIAAVHDLGSSLPDGKRLEDSQYGKRVTVEAPAG
                     YLDDVGLGDSIALNGACMTVTALDATMRCFTVEISAESLDKTAGLAQPGPINLEKAIR
                     ANDRLGGHIVAGHVDGIGTVTRFAQVGESWELRLAAPRALGKYLAYKGSIAVNGVSLT
                     VNSVIDGPDATEASINLIAHTVQNTALGHLKAGSHVNLEIDLIARYVERMLTAQPPTP
                     KASA"
     gene            complement(7004..8110)
                     /gene="ribD"
                     /locus_tag="VGA59_16945"
     CDS             complement(7004..8110)
                     /gene="ribD"
                     /locus_tag="VGA59_16945"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX9721408.1"
                     /translation="MEALDPFMRQALAQARQAVGLSEPNPRVGCLIVAPDGRVLGQGH
                     TQRAGEAHAEIMALRDAASQGHPVAGATAYVTLEPCAHHGRTGPCCDALAAAGVAKVV
                     ASLADPNPLVAGQGLARLRAAGVEVVTGPGAAESRELNIGFFSRMVRRTPWLRMKIAA
                     SLDGKTALDNGASQWITSEAARADGHAWRAQAGAVLTGIGTLLEDNPRLDVRLAPAAR
                     QPDAVVVDSRLETPPDAAIFIPGRRLLIYAAVQDARRQAALEARGAVVVCLPGAGGKV
                     DLAAMLRDLAQREINEVHAEAGHKLNGSLIRERLVDELLVYLAPKLVGQGRQMAHFGP
                     LADLAEVVAFEFLSSRMVGPDLRLLARIPGRDKF"
     gene            8257..8646
                     /locus_tag="VGA59_16950"
     CDS             8257..8646
                     /locus_tag="VGA59_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEX9721409.1"
                     /translation="MERGKQRGFTLMELMITVAIVGILGAIAYPSYQNAMVKNRRATA
                     QAHLSDLAQRQQQFLMDNRAYTSTVNTLASTPADVSRFYNVSITVAASTPPSFTATAA
                     PISPGPQAGDGALSITHTGAKSPADKW"
     gene            8655..9170
                     /locus_tag="VGA59_16955"
     CDS             8655..9170
                     /locus_tag="VGA59_16955"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX9721410.1"
                     /translation="MESIFATRNAAAGPAGARARRPSSGFTIIELLMVVVILAIMAAL
                     AAPSMSRLVATQRLKSVATDLHLALVKARAEAIKRNASVTVSPTDGSWTAGWSMAETT
                     TPDLRLDVRGPSSSVSISTTATQVIYRETGRTTPAAGVSFVITSTRTDAARCLSIDPS
                     GRPYLKEGSTC"
     gene            9164..9766
                     /locus_tag="VGA59_16960"
     CDS             9164..9766
                     /locus_tag="VGA59_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEX9721411.1"
                     /translation="MLSGRCTRVRGNSARRERGAALLEVMVSILITSFGLLALAGLQT
                     KMNSALLEAYQRAQGLVLLEDMTQRIQANQSSHATYIAASLGTGDAPQVDCSGLATRA
                     LIDRCEWSNALKGAAEISDADANLGAMIGARGCIEQLQAANAASGVCQPAIYRVTVAW
                     QGFNATVAPAVSCGVDQYGEDDSLRKAVSSRVIVPLPSCS"
     gene            9763..>10557
                     /locus_tag="VGA59_16965"
     CDS             9763..>10557
                     /locus_tag="VGA59_16965"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEX9721412.1"
                     /translation="MKKLGVPTGSPPRLTNRGMSLVELLVAIGIGTLIVAAMALLFAS
                     NSRSRADTERASRKIENGRYAVEVLRGELFHAGYLAEFDPTGLAPTLPVTKPDPCATD
                     LTSLRAALGVHVQGYDNMAATTLSCLSDVKVGTDAVVFRRASGCVVGTAGCTALAAGA
                     PAFQASSCTNAMELGSGVVANYYKLDTDTSGLTLTRRNCADAAEVRRYVLRIYYVANN
                     DKPGDGIPTLKVRELGAGGFSTASLVQGAENLQLEYGLDTDSNGDAD"
BASE COUNT         1661 a         3450 c         3604 g         1842 t
ORIGIN      
        1 aaacgtacat ccatccaacg cgccgagggt atcgcggatg tgtagacaca agatgaagcg
       61 cggttaccga ctgtagcgtt cagcccttgg gcttgaattt gatcaatcgc gcggccggaa
      121 gtgaacagga aattccggca ccaccgtgcc gtcagtatcg cgaggcaaga cctccgtctt
      181 gtcgatggcc cgcaagaccg cttcgtccca tgacttgacg ccgctggact tgatgagttt
      241 gcgcccgacg atcgtgccat cgggcgccac gcgtaattgc acagtggcgg cgggatttcc
      301 ggaaacaatc tctgtgaaga ctatattcgg tttgatccgc gcagcgattc gccccgcgta
      361 gctgttggag gggcccgccg agcgctgagc ggtgcccgtc gcggtggggc ttccggtggc
      421 gccggccatg ccctgaattc ggcgcatgtt gtcctcgcgc agggccgcga gtttcttttc
      481 ctgctccagc tgctgctgtt tggccttcgc ggccttgacc tgttcgtcct tttttttctt
      541 ctcttcggcc gcctttttct cggcgagttg ctccttcttc agttcttcct gcttcttgcg
      601 ggcttccagc ttcttctcgc gctccagttc ctcgcgcttc ttgcgagcct ccagcttctg
      661 ctggcgctcc agctcctcct gcttgcgcag cgcctgttcc ttcttcagct tttcgcgctc
      721 cagggcgata tcggcctggc tcggcgccgg tgggggcggt ggcggagcaa cctgttgggc
      781 ggggggctcc ggcgggggcg gcgggggcgg gggtgggggt ggggattgga cttccggcgg
      841 ggcggcttgc cgcggcacgc tggaccagag ctcggcttcg gccgtgaggt tgtcggattc
      901 gcggttccag ttgatgcccc aggtcagcgc cagcatgagc agcagatgcg caatcagcgc
      961 gagcgcgaac gcgcgcaccc ggcccggctg cgggggcggt gcgaactcga ggcggtcgat
     1021 gacggcggac atggggtggc tgtcgactgc ctggctatcg ggccagctgg acggagagcc
     1081 cgacgcgttt cacgccggct tgctgcagct tgtccatcac cttcacaacg ctctcgtact
     1141 tgacgttgcg gtcggcgctg attaccaccg cgctggactc ggccggccgg cccgcctggg
     1201 cctgggtgac ggccgccacc actgcttcca gcggcaggcg cgaggtgttc atgtcgatct
     1261 tcaggtcgag tgactcgtcc ctgccgatga ccacctggat cacctggtcg ggctggcgcg
     1321 gcgctttggc cacgctgggc aggtcgatca tgctgggcgc gatcaacggc gccgtcacca
     1381 tgaagatgat caacagcacc agcatgacgt cgatgaacgg caccatgttg atctcgctga
     1441 tggtgcggcg tcgtgcgcgt ccgctggtaa ccatcacagg catggcgggt cctccggctc
     1501 tagtgccccg acgccgacgc cgaagcggac gccgacgccg attgcgcgcc caggttgcgg
     1561 tgcaggatgt tggagaactc ctcgatgaag gtctccagct tgatggccac gcgatcgatg
     1621 tcgcgcgcga accggttgta ggcgaccacc gccggaatcg cggcgaacag gccgatggcg
     1681 gtggccacca gcgcttcggc gatgccgggg gccacggtgg ccagggtcac gctctgcagg
     1741 ctggccaggc cggtgaaggc atgcatgatg ccccacacgg tgccgaacag gcccaggtag
     1801 ggcgacacgg acccgaccga ggccaggaac gagaggtggg tttccacggc gtccacttcg
     1861 cgctggtagc tggcgcgcat cgcgcggcgg gcgccgtcga gaagggcgct cacgtcggtg
     1921 acccggcgct cgcgcaactt ctggtactcg cgcatgccgc tggcgaagat gcgctccatc
     1981 ggcccggcca gcttggcgtt ctgcgcggcg gcggcgaaca ggtcgttcag gctgatgccg
     2041 gaccagaagt cgcgttcgaa atcgtcgttg agcgcgttga cgcgcttgag ggcgaagagc
     2101 ttgcggaaaa tcgcagccca gctggcgatc gacacggcca ccagcagcag catgacgagc
     2161 tgcactaccc agctggcatt gagcaccagt tgaaggatcg acaggtctgg tttcatcgtt
     2221 tcaaggcttc caggatggtg ggcgggattc tcgcaggctt caggttgccg gcttcgaccc
     2281 agccgatgcg gatcgtccct tcgcatagta aggcccccgc gctcgtggcc acggcgctgg
     2341 ccccggatgt tgccgtgccc acctgcagcg ccccggccgt gccctgcctt cccttgcctt
     2401 cgcgcagaaa agcctgttgg gcgattatca aagacgcgcg gcccatttct tcgatgcacg
     2461 ccgtaacaag aagttcatca tcgagccggg ccgggtgatg gaaccgcacg gaggtttcac
     2521 ccacgacaaa caggcccccc gtgttttccc tgagcgtgcg ctgctcgaga ccgagcgagc
     2581 gcagccactc cgtgcgggca cgttcgaaga acttcaggta attggcgtaa tacacgatgc
     2641 cgcccgcatc ggtgtcttcc cagtagacgc ggatcggaaa ggcaaagacc ggcctggcgt
     2701 gaatcgggtg cgtcatgcca tgagtgccgc gaggcgggct gccgcttcgt gcaactgggc
     2761 catcgcgttc gcggtggaaa accggatgaa gtcgtgcgtc tgcgcgctgc cgaaatcgcg
     2821 gcccggcgtc acggcgacat gggcgcgccg catgacctcg aaggcgaaat cccagctgtc
     2881 cttgacgccc aggcgttcgc acgccccgga acagtcggcc caggcgtaga aggcaccgtc
     2941 cggcgccacc ggcacggcaa ggcccatcgc gcccagctgc ggaatgaacc agtcgcgccg
     3001 cgccttgaac tgggcccggc gccgctcgta ttcggccagg ctttccgctt cgaagcaggc
     3061 cagcgccgcg tgctgggcca cggcgctcgg gcagatgaag aggttctgcg ccagccgctc
     3121 gatcaccggg accagggctt cgggcacgac catccagccc aggcgccacc cggtcatgtt
     3181 gaagtatttg gagaaactgt tgatgctgac gacctggtcg tcgagggcca ggcccgtatg
     3241 gccgaaggtt tcgtcgtagc tcagtcccag gtagatctcg tccagcagcg tgatgccgcc
     3301 cctgccgctc acgaactcgt ggatgcggcg cagttcatga ggatggatcg acgtgccggt
     3361 ggggttggac ggcgaggcga gcaagacgcc tcgcgtcccc ggattccagt gggcttcgac
     3421 cttggcggca ctgagctggt agcgctcggc ggccgtggtc ggcaccagga tggcccggcc
     3481 ctccgcggcg ctgacgaaat ggcggttgca gggatagctc gggtcgggca tcaggatctc
     3541 gtcaccggct tcgatcaggg ccaggcacgc cagctgcaat gccgccgatg cgccggccgt
     3601 cacgatgatc cgccgcgcgg gtaccgccac gccgaagcgc cgcgcatacc agtcgctgat
     3661 gcgttcgcgc aaggtgtcca ggccggtggc ctgcgtgtac tgggtcaggc catcgcgaat
     3721 ggccttggcg gcggcttcct gcaccagggg cggagccgtg aagtccggct ctccgatgtt
     3781 caggaagatc atgggccggt cgctgtgggc cacttcgcgg gcgagctgcg atgccgcctt
     3841 ggccacttcc atgacataga aaggctcgat acgccgggcg cgcgcggtaa ttctcattca
     3901 ggcctccgcc gggcggcgcc ggggcgggaa cgcgctcatc gggccttccc gctggccttg
     3961 tccgcctcga cttcggccgg gcggagcgag ggcgccagcc cattgagcac cgcattcacg
     4021 tatttgtgtc cgtccgtgcc gccgaactcc ttggccagct cgatgcattc gttgagcacc
     4081 acgcgccacg gcacgtccgg gcaatgcagg aactcgtagg cgccgatcca catgacgcca
     4141 tgctcgatcg gcgagatttc ctcgagcttg cggtccagca gcggcacgat cagcgcgtcg
     4201 agttgtgccg cctcgccggc gcagccatgc agcagcgcgt cgtagtgcac cgagtcggcc
     4261 ttgtggaaac cggccaggtc gcgcgtgaac aggtcgatcg cccgcacctc gttgcggccg
     4321 accaggtact ggtaaagcgc ctgcaatgcg aattcacggg cccggctgcg tgccgacttg
     4381 gccgaagcct tccgggcgcc ggtgctcgtc aggcccgcac gggcctggcg cggcgcgcgt
     4441 tccgaggccg gattgccgtc gccgctcatc ccaaatggtc caggaggttg cacatttcga
     4501 ccgcgacgcg cgcgccgtcg cgcccctttt cggtctgccg ggccaccgcc tgctccacgt
     4561 tctcggtggt gaggatcacg ttcgcgatgg ggagctggta gtccagcgac acgcggttca
     4621 ggcccgccgc ggattcgttg gccacgagtt cgaaatggta ggtttcgccc cggatgatgc
     4681 agcccagggc gatcaatgcg tcgtagccgc ctttttcggc caggccctgc agtgcgatgg
     4741 gcacctcgag ggcgccgggt acgcagacat ggtcgacgtg gtcgtccgcg acgcccagcg
     4801 cgcgcagttc ggcccggcag gcattcgcca gggcattggt gatgctctca ttgaagcggg
     4861 cctgcacgat gcctatgtac aggcctttgc cgtccaggtt cgcggccgtg cctttctctg
     4921 cgccgaacat tgcttgtgct ttccggagat tggttgtcag ggggcgatgt agccggcgat
     4981 ttccaggcca tagccggcca tgctgggcat gcggcgcgga ttgcccatga ggttcatgcg
     5041 gtgcacgccg cattcacgca ggatctgcgc gcccacgcca taggtgcgca ggtccaggcg
     5101 gccgcgttcg ggggcgtgcg aggcccgggc cttgccttcg aactgcgcca gcagctgctg
     5161 cgcgtcctcg ccgcaattga gcaggacggc cacgcccttg ccctcgagcg cgatccggcg
     5221 caggctggcg tccaggttcc aggaatgcat ggagcggttg atctcaagcg cgtccagcat
     5281 cgacatcggc tcgtgcacgc gcaccgcgac ggtctcgtcg ggcgaccagt cgcccttgac
     5341 cagcgcaaag tgcaggccgt ggctgggctt gtcccgccag gcatgggcca tgaattcgcc
     5401 atgcacggtc ttcagcggcc gcgaaccgat cttctcgatc agcgactcgt gccggctgcg
     5461 gtgctcgatc aggtcggcga tggtgccgat cttcaggccg tgctgggcgg cgaacagctg
     5521 caggtccggc agccgcgcca tggtgccgtc gtccttcatg atctcgcaga tcacggccgc
     5581 gggcgtgagc ccggccatcg ccgccaggtc gcagcccgcc tcggtgtggc cggcgcgcat
     5641 cagcacgccg ccctcgacgg cctgcagcgg aaagatgtgg cccggctgca ccaggtcctc
     5701 ggccctggcg ttcttggcga ccgccgcctg cacggtgcgg gcgcggtcgg cggccgagat
     5761 gccggtggtg acgccctcgg ccgcttcgat cgagacagtg aaggctgtcc ctttcttgtc
     5821 gccgttgcgc gtggccatgg ggggcaggcg caggcgctcg cagcgcttgc gcgtgagcgt
     5881 caggcagatc aggccgcgcc cgaagcgcgc catgaaattg atggcttccg gcgtgacatg
     5941 ctcggcggcg agcagcaggt cgccttcgtt ctcgcggtct tcctcatcga ccaggatgac
     6001 gatgcggccg gccccgatgt cggcgatgat gtcctcgacc ggggagatgg atacgggagc
     6061 gaggtcggga gaggaagcgc tcatgcactg gctttcgggg tggggggctg tgcggtgagc
     6121 atgcgctcga cgtagcgggc gatcaggtcg atctccaggt tcacatggct gccggccttc
     6181 agatgcccga gcgccgtgtt ctgcaccgta tgcgcgatca ggttgatgct ggcttccgtc
     6241 gcgtcggggc cgtcgatgac ggaattgacc gtgaggctga cgccattcac ggcgatcgag
     6301 cccttgtagg ccaggtactt gccgagcgcg cggggcgccg ccagccgcaa ctcccagctt
     6361 tcgccgacct gggcgaaacg cgtgacggtg ccgatgccgt ccacatggcc ggccacgatg
     6421 tggccgccca ggcggtcgtt ggcgcgaatg gccttctcga ggttgatggg cccgggctgc
     6481 gccaggccgg cggtcttgtc cagcgattcg gcggaaatct cgacggtgaa acagcgcata
     6541 gtcgcgtcaa gggcggtgac cgtcatgcag gcgccgttga gcgcgatgct gtcgccgagg
     6601 ccgacgtcgt cgaggtagcc ggcgggcgct tccacggtca ctcgcttgcc gtattgcgaa
     6661 tcctcgaggc gcttgccgtc aggtaaagag cttcccaggt cgtggacggc ggcgatgcgc
     6721 cccacgccgg taatgattcc ggtgaacatg gcggtatttt cgcagggatt ggaaaggagg
     6781 gtcgggaatg agaaggatgt cattgcgggc tgggctcgcg gaacgcccgg ggcgcgcagc
     6841 tgtccagtgt agcgagccca atgccgggcc tacgctatgc acgtgccgag gcatcgcgct
     6901 ccggcccggc cccgcaatcc atcccggcgg caggcttgtg gggcatgaag gcatggatcc
     6961 cgggtcaagc ccgggatgac aaattcctgg gtgcagggcg aatctagaac ttatcgcggc
     7021 cggggatgcg cgccagcagg cgcaggtcgg gcccgaccat ccggctcgac aggaattcga
     7081 acgccaccac ctccgccagg tcggcgagcg ggccgaaatg cgccatctgc cgcccttgac
     7141 ccacgagttt gggggccagg tacaccagca attcgtcgac cagtcgctcg cggatcagcg
     7201 agccattgag cttgtggccg gcctccgcat gcacctcgtt gatttcacgc tgcgccaggt
     7261 cgcgcagcat cgccgccaga tcgaccttgc cgcccgcccc gggcaggcaa acgaccaccg
     7321 ccccacgcgc ttccagcgca gcctggcgcc gggcgtcctg gacggcggca tagatcagga
     7381 ggcgccggcc cggaataaag atggcggcgt ccggcggcgt ctccagccgg ctgtcgacca
     7441 ccaccgcgtc gggctggcgt gccgccgggg ccaggcggac gtcgagcctg gggttgtctt
     7501 cgagcagggt gccgatcccg gtcagcacgg cgccggcttg cgcccgccag gcgtggccgt
     7561 cggcgcgcgc ggcttccgac gtgatccact ggctggcgcc gttgtccagc gcggtcttgc
     7621 cgtccagcga ggcggcgatc ttcatgcgca gccacggcgt gcgtcgcacc atccggctga
     7681 agaacccgat attgagttcg cgcgattcgg ccgccccggg tccggtgacc acttccaccc
     7741 ccgccgcgcg aaggcgcgca aggccctggc ccgcgaccag cggattcggg tcggcgagcg
     7801 aggcgacgac cttggcgacg cccgcggcgg ccagggcatc gcagcagggc cccgtgcggc
     7861 catggtgcgc gcagggctcg agcgtcacat aggccgtggc gccggccacc ggatggccct
     7921 gggaggcggc gtcgcgcagc gccatgattt ccgcgtgggc ctcgcccgcc cgctgcgtgt
     7981 ggccttggcc cagcactcgc ccgtcggggg ccacgatcag gcaacccacg cgcggattgg
     8041 gttcggacag gcccaccgcc tgccgggcct gcgccagggc ttgccgcatg aaggggtcga
     8101 gggcttccat cgctgcctat tctccggcat ccaggggcgc aagcggctcg aaggaccatt
     8161 cgggccgtca aagcaaccgg tcgtcgggtg ggtccttgcg gcaagaagaa ggtcggttat
     8221 tgttcagcta gaccaatggg caatacagga gccggcatgg aaagaggcaa gcagcgggga
     8281 ttcacgctga tggagttgat gatcacggtg gcgatcgtcg gcatcctggg tgcaatcgcc
     8341 tacccctcgt accagaacgc catggtcaag aatcgccgtg caaccgcaca ggcgcacctg
     8401 tccgacctgg cccagaggca acagcaattt ctgatggaca accgggccta cacgtccacg
     8461 gtcaacacct tggcgagcac acccgccgat gtcagccggt tctacaacgt gagcatcacc
     8521 gtggcggcaa gcacgccacc gtcctttacc gcgaccgcgg cccccatttc ccctgggccc
     8581 caggccggcg acggcgcatt gagcatcacc cataccgggg ccaagtcgcc ggccgacaag
     8641 tggtagaggt catcttggaa tccatttttg ccaccaggaa tgcggccgca ggccctgcgg
     8701 gcgcgagagc gcggcggccc agcagcggct tcaccatcat cgaattgctg atggtggttg
     8761 tcatcctggc gatcatggcg gcgctggcgg caccctccat gagccggctg gttgcgaccc
     8821 agcggctcaa gtcggtcgcc acggatctgc atttggccct ggtcaaggcg cgggctgaag
     8881 ccatcaagcg caatgcgagc gtcacggtga gtccgaccga tggcagctgg acggcgggct
     8941 ggtccatggc ggaaaccacg actcccgacc ttcggctcga tgtgcgggga ccatcgagca
     9001 gcgtgagcat ttcaacgact gcgacgcagg tcatttaccg cgaaaccggc agaaccacgc
     9061 ctgccgccgg cgtgtccttc gtcatcactt cgacgcgcac ggatgccgcg cgatgcctgt
     9121 cgatcgatcc cagcggccgg ccctatctca aggagggttc cacatgctga gtggaagatg
     9181 cacgcgggtt cggggtaata gcgctcgccg agagcgcggt gcggccctgc tggaagtgat
     9241 ggtctcgatc ctgattacct cgttcggtct gcttgcgctt gccggcttgc agacgaagat
     9301 gaattcggcg ctgctggagg cgtaccagcg ggcgcaggga ctcgtcctgc tggaggatat
     9361 gacccagcgc atccaggcga accagagttc gcacgcgacc tacattgcgg cgtccctggg
     9421 gacgggagat gcgccgcagg tcgattgttc aggcctggct acccgcgctc tcatcgaccg
     9481 ctgcgaatgg agcaatgcgc tcaagggcgc agccgaaatc agcgacgcgg acgccaacct
     9541 gggcgccatg atcggcgccc gcggctgcat cgagcagttg caggccgcga acgcggccag
     9601 cggcgtctgc cagccggcca tctaccgggt cacggtggca tggcaggggt tcaacgcaac
     9661 cgtcgcaccc gcggtgtcgt gcggcgtcga tcaatatggt gaggacgaca gcttgcgcaa
     9721 ggcggtgtcg tcgcgcgtga tcgtgccgtt gccgtcctgc tcatgaaaaa actcggtgtt
     9781 cctaccgggt cgccgccgcg gctcacgaac cgtggcatgt cgctcgtcga attgctcgtg
     9841 gccatcggga tcggtacgct gatcgtggcc gcgatggccc ttctgttcgc aagcaacagt
     9901 cgcagccggg ccgacacgga acgcgccagc cggaagatcg aaaacggcag gtatgccgtg
     9961 gaggtcttgc gcggcgagct ctttcacgcg ggctatctcg cggagttcga tccgaccggc
    10021 ctggccccga ccttgcccgt caccaagccg gacccctgcg ccacggatct gaccagcctg
    10081 cgcgcggccc tcggcgtgca tgtccagggc tacgacaaca tggcagcgac cacgttgagc
    10141 tgtctcagtg acgtcaaggt cggtaccgat gccgttgtgt tccgcagggc gagcggctgt
    10201 gtcgtgggga cggcgggctg caccgccctc gcagccggcg ctcccgcatt ccaggcatcc
    10261 tcgtgcacca acgccatgga actcggctcc ggcgtggttg ccaactacta caagctcgat
    10321 accgatacct ccggcctgac actgaccagg cggaattgcg cggacgctgc cgaagtgcgg
    10381 cgttacgtgc tgcgcatcta ctacgtggcc aacaatgaca agccggggga cggcatcccc
    10441 accctcaagg tccgagaact gggcgccggt ggcttttcaa ccgcttccct ggttcagggc
    10501 gcggagaatc tccagctcga atacgggctg gacaccgaca gcaacggcga tgcggac
//
LOCUS       DASWIY010000473        10094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_327126, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000473 DASWIY010000000
VERSION     DASWIY010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10094
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327126"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /gene="aceE"
                     /locus_tag="VGA59_16970"
     CDS             complement(<1..634)
                     /gene="aceE"
                     /locus_tag="VGA59_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747281.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX9721413.1"
                     /translation="MSAQPKDPFGSAANDADAQETREWIDALSAVIEAEGPERGHFLL
                     EQLLEQARQEGIDLPFSATTGYVNTIEPEQEERCPGNLDVEERLRAYMRWNAMAMVVK
                     ANRVHPEDGGDLGGHISSFASLATMFGAGFNHFWHAESEGHGGDCLYIQGHSSPGIYA
                     RAYMEGRLTEEQLLNFRQEVDGKGLSSYPHPKLMPEFWQFPTVSMGLGPLM"
     gene            860..3394
                     /locus_tag="VGA59_16975"
     CDS             860..3394
                     /locus_tag="VGA59_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9721414.1"
                     /translation="MQDPVTAPETHEAAAPLAWWRRWWRRQSPVHQDRFAMLAPLAAV
                     MLFLAAIVAAFGYLRLEEMDREQEAVKRDVEYAHQRVRLRLLERQEQLMRIARDISNK
                     EVDPDEFVGRGESMVSQYPELQAVTWIDERRRIKASYGAPSLASHQLRKVRDVLRPGE
                     TENIYSLARDLQQPVYSQPAAGTDSTGLLQLHIPLAEQGRFSGVILGEYSIDGLLRYG
                     VPAEVSAKYAVALLDGKGRVLAGSSVPARNPASQLLPWAPQSNEYEVPVSPVGNGLII
                     RAQAYRTSLGVIGSGLFWLVGALSAMTAWMLIGNWRHTRRRMQAQQALVAETNFRRAM
                     ENSMLTGMRALDMQGRITYVNAAFCQMTGWSEAELVGRTPPFPYWPEGDREQLAARLE
                     DELSGRTVSGGFQVRVKRKDGTLFDARLYVSPLVDARGHQTGWMTSMTDITEPNRIRE
                     QLSASYERFTTVLEALDASVSVAPLGSEELLFANKLYRLWFGVQTVGHLQMVAQAGVP
                     EHPRNDESADEVDSFVGLPTGGLTLAQSENAEIFVPELGKWLEVRSRYLNWVDGRLAQ
                     MVIATDITPRRHAEEQSSAQAERAQSASRLITMGEMASSVAHELNQPLTAINNYCHGM
                     VSRIRGKQINEEDLLAALDKTAHQAQRAGQIIQRIRSFVKRSEPNRSLSDVTLIVGEA
                     VELAEIELRRRNVRLSHYVAARLPKVMVDPILIEQVLVNLLKNAAESIEHAGRPASRR
                     SVELRVIPKQVDEQPVVEFSVLDSGQGLAPEVMDRLYEAFFSTKVEGMGIGLNLCRTI
                     VESHQGRMQAENIYNGPEVAGCRFSFWIPVSGATNSIVGKNTGVTA"
     gene            3435..4061
                     /locus_tag="VGA59_16980"
     CDS             3435..4061
                     /locus_tag="VGA59_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9721415.1"
                     /translation="MSLIPKKGTVYVVDDDEAVRDSLQWLLEGKDYRVRCFDSAESFL
                     SRYDPREVACLIVDIRMGGMTGLELQDRLLERKSPLPVVFITGHGDVPMAVNTMKKGA
                     MDFIQKPFKEDELVSLVERMLEHAKGAFAEYQSAASRDALLSKLTSREAQVLERIVAG
                     RLNKQIADDLGISIKTVEAHRANIMEKLNANTVADLLKIALGQNAPKS"
     gene            4153..5004
                     /gene="folD"
                     /locus_tag="VGA59_16985"
     CDS             4153..5004
                     /gene="folD"
                     /locus_tag="VGA59_16985"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX9721416.1"
                     /translation="MTAQLIDGNALSKKIRAEVAQRAAALKARGVTPGLAVILVGEDP
                     ASQVYVKHKVNDSREAGLNSVLERYPATLGEAQLLARIRALNEDPSIHGILVQLPLPK
                     HMDTNKVIETISPAKDVDGFHVASAGALLIGQPGFWPCTPYGCMKMLETIGYELRGKH
                     AVVIGRSNIVGKPMAMMLLRKNATVTICHSATKDLKGHTLQADLVVAAVGKRNMLTAD
                     MVKPGAVVIDVGMNRNDEGKLCGDVDFAGVSEVAGWITPVPGGVGPMTRAMLLVNTLE
                     AAERAAA"
     gene            5227..7272
                     /locus_tag="VGA59_16990"
     CDS             5227..7272
                     /locus_tag="VGA59_16990"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX9721417.1"
                     /translation="MTNPLLDFTDLPLFDQVEPQHVAPALDSLLAAAQKALETVTQPE
                     FPSRWEDIAKVLDTATEYLGRAWGVVSHLNGVADTPQLRAAYNEALPRVTDFWTRLGS
                     DERLYAKYKAIDPATLNAEQRQAHRNAIRNFVLGGAELKGAARERFAAIQERQAELGQ
                     KFSEHALDATDAFGYYASESELAGVPQDVVLAARAAAQAEGKEGYKLTLKMPSYLPVM
                     QFARSSTLRERLYRAYVTRASDQGDPKFDNSALIGEILALRREEARLLGYPNFGEMSL
                     VPKMASSPRQVVDFLRDLARRARPYAEKDVADMRAFGAANLGIADPQAWDWSYIGEKL
                     KEERYAFSEQEVKQYFTAPKVLAGLFKIIETLFEVTIRRDVAPVWNPSVEFYRIERER
                     RVGERLSTELVGQFYLDPAARTGKRGGAWMDDVRARWLRPDTGRLQTPVAHLVCNFAE
                     GVEGKPALLTHDDVITLFHESGHGLHHMLTQVNEHDVSGISGVEWDAVELPSQFMENF
                     CWEWNVLRHMTAHLETGAALPRELFDKMLAAKNFQSGLQTLRQIEFALFDMLLHTEHD
                     PAQDFMPLLAKVREEVAVIQPPAFSRTAHTFSHIFAGGYAAGYYSYKWAEVLSADAYA
                     AFEETMGKDGLPSIETGRKYRQAILETGGSRPAIESFRAFRGRDPSLDALLRHQGMA"
     gene            7302..7955
                     /locus_tag="VGA59_16995"
     CDS             7302..7955
                     /locus_tag="VGA59_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX9721418.1"
                     /translation="MFSARPLLAITGLAGLLAMAGFSSSDANAQQIYRIVEPGGRITF
                     SDKASLDANARASIAAAVPMPGAGDYGALPFELKQAASRYPVTLYTGPGCAPCLAGRA
                     LLTGRGIPFAEKTVTTSEDIEALKRLAGAPSLPFLTIGGQQLKGFSEVEWVQFLDAAG
                     YPKTSQLPSSYTPPPATALVTAQQPEATNPTAPPAPPRAAAPAPAARSADNPAGIRF"
     gene            complement(7956..8627)
                     /gene="rpiA"
                     /locus_tag="VGA59_17000"
     CDS             complement(7956..8627)
                     /gene="rpiA"
                     /locus_tag="VGA59_17000"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX9721419.1"
                     /translation="MTQDELKTLVGRAALQYVVPGEIVGVGTGSTVNKFIDALATMKD
                     RIAGAVSSSNASTERLRALGIAVFDANDAGDIPVYIDGADEIDGHGFMIKGGGAALTR
                     EKIVAAQSKRFVCIADESKLVEVLGRFPVPVEIIPMATRRLVRQFAAMGATAAVRLRD
                     GQPLVTDNGQHILDVAGLAISDPLGFESEVNQWPGVVTVGVFAHQKAAVCLLGTPGGV
                     RTLAF"
     gene            8668..9732
                     /locus_tag="VGA59_17005"
     CDS             8668..9732
                     /locus_tag="VGA59_17005"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX9721420.1"
                     /translation="MSLLPYSLARPFLFGLDPETAHELTMQSLARLQGTPLEWAYRNG
                     LVEDPVELAGLTFPNRVGLAAGLDKNGRCIDGLGAMGFGFVEVGTVTPRPQPGNPKPR
                     MFRLPQANALINRLGFNNDGLEAFITNVKRSSFRKKGRILGLNIGKNAATPIANATGD
                     YLVCLDGVYPHADYVTINISSPNTADLRSLQGDQALGGLLGAIAKRREELSREHGRRV
                     PIFVKIAPDLDRAQVNAIAAALKRHGMDGAVATNTTTSREAVQGLPHAQETGGLSGAP
                     LLEASNRVIGQLRSALGKGFPIIGVGGVMSAQDAVSKIRAGADAVQIYTGLIYRGPQL
                     VSDAARALKALSCQPTTQKA"
     gene            complement(9720..>10094)
                     /locus_tag="VGA59_17010"
     CDS             complement(9720..>10094)
                     /locus_tag="VGA59_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9721421.1"
                     /translation="PCTVPTQFIPAGNRIPGTAQSVSAAEIAWQPARGWRAGAELRRS
                     SRVHVNDTNSEAAPTFTTLALHAGYVFDLRGWALAAHVRVDNVLDRRYAGSVIVNEGN
                     GRFFEAAPGRGYVLKLAGNYAF"
BASE COUNT         1801 a         3321 c         3331 g         1641 t
ORIGIN      
        1 ccatcagcgg gcccaggccc atggacaccg tcgggaactg ccagaactcg ggcatcagct
       61 tggggtgcgg gtagctcgaa aggcccttgc cgtccacttc ctggcggaag ttcagcagct
      121 gctcctcggt caggcgccct tccatgtagg cgcgggcgta gatgccgggc gagctgtggc
      181 cctggatgta caggcaatcg ccgccgtggc cttcgctttc ggcatgccag aagtggttga
      241 agccggcgcc gaacatggtg gccagcgagg cgaaggagct gatatggccg cccaggtcgc
      301 cgccgtcctc gggatgcacg cggttggcct tgaccaccat cgccatcgcg ttccagcgca
      361 tgtaggctcg caggcgctcc tcgacatcga ggttgccggg gcagcgctcc tcctgttccg
      421 gctcgatcgt gttgacatag cccgtggtgg cggagaacgg caggtcgatg ccctcctgcc
      481 gcgcctgctc caggagttgc tccaggagaa aatggccgcg ctccgggcct tcggcttcga
      541 tgacggccga aagggcgtcg atccattcgc gggtttcctg tgcgtccgcg tcgttcgcgg
      601 ccgagccgaa ggggtctttg ggctgtgctg acatcttggt ctcctgttgc cttacctggc
      661 gaagtctcac ctaatttagt gcggttccgt ttccgagcgc ggagtgtcgc acagttttta
      721 tgtcatttca aatggtgcat gtagttttca taatgtggtt ataccaagga gtgacggcca
      781 acgagtaacc gtgcgcaccg ccgggaactc gcggcggcca gagggacttc ggcgcgcctg
      841 ggcctacact tggagcctca tgcaagaccc cgttaccgcc cccgagacgc atgaggcagc
      901 ggccccgctt gcctggtggc gccgctggtg gcggcgccag tcgcccgtgc accaggaccg
      961 cttcgccatg ctggcgccgc tggcagccgt catgctgttc ctggcggcca ttgtcgcggc
     1021 cttcggttac ctgcgcctgg aggaaatgga ccgcgagcag gaggccgtga agcgcgacgt
     1081 cgaatacgcg caccagcggg tccggctgcg cctgctggaa cgccaggagc agctgatgcg
     1141 catcgcgcgc gacatctcca acaaggaagt ggatcccgac gagttcgtgg gccggggcga
     1201 atccatggtc agccagtacc ccgagctgca ggcagtcacc tggatagacg agcgccgccg
     1261 catcaaggcc agctatggcg cgcccagcct cgccagccac cagctgcgca aggtgcggga
     1321 cgtgctgcgt cccggcgaaa cggaaaacat ctacagcctt gcgcgcgacc tgcagcagcc
     1381 ggtgtattcg cagccggcgg cgggcaccga cagcacggga ctgctgcagc tgcatatccc
     1441 gctggctgaa caggggcggt tttccggtgt catcctgggc gagtattcaa tcgacggatt
     1501 gctgcgctat ggggttccgg cggaggtgtc ggccaagtac gcggtggcgc tgctggatgg
     1561 caagggccgc gtgctggcag gcagttcggt gccggcacgc aaccccgcca gccagctgct
     1621 gccctgggcg ccccagtcca acgaatacga ggtccccgtc tctccggtgg gcaacggcct
     1681 gatcatccgg gcgcaggcct atcgcacttc gctgggcgtg atcggcagcg gcctgttctg
     1741 gctggtgggg gcgttgagcg ccatgacggc ctggatgctg atcggcaact ggcgccatac
     1801 gcgccgccgc atgcaggccc agcaggcgct ggtggccgaa accaatttcc gccgcgccat
     1861 ggaaaattcc atgctcaccg gcatgcgggc actggacatg cagggccgca tcacctacgt
     1921 gaacgccgcg ttctgccaga tgacggggtg gagcgaggcc gaactcgtgg gccgcactcc
     1981 cccttttccc tactggcccg agggcgaccg cgagcagctt gcggcgcggc tggaggatga
     2041 gttgagcgga cgcaccgtgt cgggcggctt tcaggtgcgc gtcaagcgca aggacggcac
     2101 gctgttcgat gccaggctgt acgtgtcgcc gctggtcgac gcgcgcgggc accagaccgg
     2161 ctggatgacg tcgatgaccg acatcaccga gccgaaccgc atccgcgagc agctgtcggc
     2221 gtcctacgag cgcttcacca ccgtgctgga ggcgctggac gcatccgtgt cggtggcccc
     2281 gctgggcagc gaggagctgc tgttcgccaa caagctgtac cgcctgtggt tcggcgtaca
     2341 gacggtgggc catctgcaaa tggtggcgca agcgggtgtg cccgagcatc ctcgcaacga
     2401 cgagtcggcg gacgaagtcg actcattcgt cggcctgccc accggcgggt tgaccctggc
     2461 gcagtccgag aacgcggaga tcttcgtgcc cgaactcggc aagtggctgg aggtgcggtc
     2521 gcgttacctg aactgggtgg acggccggct ggcccagatg gtgatcgcga ccgatatcac
     2581 gccgcggcgc cacgccgagg agcaatcgtc ggcccaggcc gaacgcgcgc agtcggccag
     2641 ccgcctcatc acgatggggg aaatggcctc gagcgtggcg catgaactga accagccgct
     2701 gacggcgatc aacaactact gccatggcat ggtgtcgcgc atcaggggca agcagatcaa
     2761 cgaggaggac ctgctggcgg cactggacaa gaccgcgcac caggcccagc gcgccggcca
     2821 gatcatccag cggatccgct cgttcgtgaa gcgcagtgag cccaaccggt cgctttcgga
     2881 tgtgacgctg atcgtcggcg aggccgtcga actggccgag atcgaactgc gccggcgcaa
     2941 cgtgcggctg tcgcattacg tcgccgcgcg gcttcccaag gtcatggtcg accccatcct
     3001 gatcgagcag gtgctggtca acctgctgaa aaacgccgcc gaatcgatcg agcatgccgg
     3061 gcgcccggcc tcccggcgca gcgtggagtt gcgcgtgatt cccaagcagg tggacgagca
     3121 gccggtggtc gaattctccg tgctcgattc gggccagggc ctcgcgccgg aggtcatgga
     3181 ccgactgtac gaagcgttct tctccaccaa ggtggaaggc atggggatcg ggctgaatct
     3241 gtgccgcacg atcgtcgagt cgcaccaggg acggatgcag gccgagaaca tctacaatgg
     3301 gcccgaagtt gccggctgcc gtttttcctt ctggattcct gtctccggcg ctacaaattc
     3361 gatagtgggc aagaacaccg gggtgaccgc atgagcccgg cgaacaccgc gttgcgcggc
     3421 acggagattc accaatgagc ttgattccga agaagggcac cgtgtatgtg gtggacgacg
     3481 acgaggccgt gcgcgactcg ctgcaatggc tgctcgaagg caaggactac cgggtgcgct
     3541 gcttcgactc cgcagagtcg ttcctgtcgc gctacgaccc gcgcgaggtg gcgtgcctga
     3601 tcgtggacat ccgcatgggc ggcatgaccg ggctggagct ccaggaccgc ttgctggagc
     3661 gcaagtcgcc gctgccggtc gtcttcatca cgggccacgg cgacgtgccg atggccgtga
     3721 acaccatgaa gaaaggcgcg atggacttca tccagaagcc gttcaaggaa gacgagctgg
     3781 tgagcctggt ggaacgcatg ctcgagcacg ccaagggcgc gttcgccgag taccagagcg
     3841 ccgccagccg cgacgccctg ctgtccaagc tcacgtcgcg cgaagcgcag gtcctggagc
     3901 gcatcgtcgc agggcgcctc aacaagcaga tcgccgacga cctgggcatc agcatcaaga
     3961 cggtggaggc gcatcgcgcc aacatcatgg aaaagctcaa cgcgaatacc gtggccgatc
     4021 tcctgaagat cgcgctgggc cagaacgctc cgaagagtta gacgataacg cgcccgctaa
     4081 agcgggacgc gtccttctaa gtgtttcgta atgggtgggc agagagtgcc catccactct
     4141 caagtccctc ccatgaccgc tcaactgatc gacggaaacg ctctctcgaa aaagatccgc
     4201 gccgaggtgg cgcaacgcgc cgccgcactc aaggccaggg gagtcacgcc cggcctggcc
     4261 gtgatcctgg ttggcgaaga cccggccagc caggtctacg tcaaacacaa agtcaacgac
     4321 agtcgggaag ccggcctgaa ttcggtgctg gagcgctacc cggcgacact gggtgaagcg
     4381 cagctgcttg cgcgcattcg cgctctgaac gaggaccctt cgatccacgg catcctggtg
     4441 cagctgccgc tgcccaagca catggacacc aacaaggtga tcgagaccat ctcgcccgcc
     4501 aaggacgtcg acggtttcca tgtggccagt gccggggcgc tgctgatcgg ccagccgggc
     4561 ttctggccct gcacacccta tggctgcatg aagatgctgg agaccatcgg ctacgaattg
     4621 cgcggcaagc acgccgtggt gatcggccgc agcaatatcg tgggcaagcc gatggcgatg
     4681 atgctgctcc ggaagaacgc cacggtgacg atctgccata gcgccacgaa ggacctgaag
     4741 ggtcacaccc tgcaggccga cctggtggtc gccgccgtgg gcaagcgcaa catgctgacg
     4801 gccgacatgg tcaagcccgg cgccgtggtg atcgacgtcg gcatgaaccg caacgacgag
     4861 ggcaagctct gcggcgacgt cgatttcgcc ggggtgagcg aagtggcggg ctggatcacg
     4921 cccgtgccgg gcggcgtcgg cccgatgact cgcgcgatgc tgctggtcaa tacgctcgaa
     4981 gcggccgaac gagccgcggc ataggctggc tcttgtcatg ccggtccggc cccggcatcc
     5041 atgacttcct ggattgcggc gccgggccgg agcgtgatgc ctcggcacgt gcatagcgta
     5101 ggcccggcat tgggctcgct acactggaca gctgcgcgcc ccgggcgttc cgcgagccca
     5161 ggccgcaatg acaacgcatt cccttacggc ccttgtttcc ggccgccctg acgcaaaatg
     5221 cgtcgaatga ccaatcccct tctcgacttc accgacctcc ccctcttcga ccaggtcgag
     5281 ccgcagcacg tggcgccggc cctggacagc ctgctggccg cggcgcagaa ggcgctcgaa
     5341 accgtgaccc agcccgaatt tcccagccgc tgggaggaca tcgccaaggt cctggatacc
     5401 gccaccgaat acctcggccg cgcctggggc gtcgtcagcc atctcaacgg cgtggccgat
     5461 acgccccagc tgcgcgccgc ctacaacgag gccctgccaa gggtgacgga tttctggacc
     5521 cggctgggct cggacgaacg cctgtatgcc aagtacaagg ccatcgatcc ggccacgctc
     5581 aacgccgagc aacggcaggc ccataggaac gcgatccgca atttcgtgct gggtggtgcg
     5641 gaactgaaag gcgccgcccg cgagcgcttt gccgcgatcc aggagcggca ggccgagctg
     5701 ggccagaagt tcagcgaaca tgcgctcgac gccacggacg cgtttgggta ttacgcgagc
     5761 gagagcgaac tcgcgggcgt gccgcaggat gtggtcctgg ccgcgcgcgc agccgcacag
     5821 gccgaaggca aggaaggcta caagctcacg ctgaagatgc ccagttacct gccggtgatg
     5881 cagttcgccc gcagcagcac cttgcgcgaa cggctgtacc gcgcttacgt cacccgtgcc
     5941 agcgaccagg gcgatccgaa gttcgacaac agcgccttga tcggcgagat cctggcgctg
     6001 cggcgcgaag aggcacggct gctgggctac cccaatttcg gcgaaatgtc gctcgtgccc
     6061 aagatggcca gctcgccccg gcaggtcgtc gacttcctgc gggacctggc tcgcagggcc
     6121 cggccgtacg cggaaaaaga cgtggccgac atgcgcgcct tcggcgccgc caatctgggg
     6181 atcgccgacc cgcaggcgtg ggactggtca tacatcggcg agaagctcaa ggaggaacgc
     6241 tatgccttca gcgagcagga ggtcaagcaa tacttcaccg cgcccaaggt gctcgcgggc
     6301 ttgttcaaga tcatcgaaac cctgttcgaa gtgacgatcc ggcgcgacgt ggcgccggtg
     6361 tggaacccct cggtcgagtt ctaccgcatc gaacgcgaaa ggcgcgtggg cgagcgcctg
     6421 tccaccgagc tggtcggcca gttctacctg gacccggcgg cgcgcaccgg caagcgcggc
     6481 ggcgcctgga tggacgacgt tcgcgcgcgc tggctgcgcc cggacacggg ccggctgcag
     6541 acgccggtcg cccacctggt ctgcaatttc gcggagggcg tcgagggcaa gccggcgctg
     6601 ctcacccatg acgacgtcat caccctgttc cacgagtcgg gccacggcct gcaccacatg
     6661 ctgacccagg tgaacgaaca cgacgtctcg ggtatcagcg gcgtggaatg ggacgcggtg
     6721 gagctgccca gccagttcat ggaaaacttc tgctgggaat ggaacgtgct gcggcacatg
     6781 acggcccacc tggaaacggg cgccgcgttg ccgcgcgagc tgttcgacaa gatgctggcc
     6841 gcgaagaatt tccagagcgg cctgcaaacg ctgcgccaga tcgagttcgc gctgttcgac
     6901 atgctgctgc acaccgagca tgatccggcg caggacttca tgccgctgct cgccaaagtg
     6961 cgcgaagaag tcgcggtgat ccagccgccc gcattcagcc gcaccgcaca tacattcagt
     7021 cacatttttg ccggcggcta tgcagcgggg tactacagct acaaatgggc cgaggtcctc
     7081 agcgccgatg cctacgccgc gttcgaagag accatgggta aggacgggtt gccgagcatc
     7141 gaaaccgggc gaaaataccg tcaagccatt ctggagaccg gcggcagccg gcccgcgatc
     7201 gaatcgttca gggccttccg tggccgcgat ccctctctgg acgccctgct gcgtcaccag
     7261 ggaatggctt gacctgatag ccactgttgg agttcatacc gatgttttct gcccgcccat
     7321 tgctcgccat caccggcctc gcgggactcc tggccatggc cggattcagc agcagcgatg
     7381 caaatgcgca acagatttac cggattgtcg agcccggcgg tcgaattact ttctcggaca
     7441 aggcttcgct ggatgccaac gcccgcgcga gcatcgcggc cgcggttccg atgccgggcg
     7501 cgggcgacta cggcgcgctg cccttcgaac tgaagcaggc cgccagccgc tacccggtga
     7561 cgctgtacac gggacccggc tgtgccccct gccttgcggg tcgggccttg ctgaccggcc
     7621 ggggcattcc cttcgcggaa aagacggtga ccaccagcga ggacatcgaa gcattgaaac
     7681 ggctcgcggg cgcgccatcc ctgccgttcc ttacgatcgg cggacagcag ctcaaaggct
     7741 tttccgaggt tgaatgggtc cagttcctgg acgccgcggg ctatccgaaa acctcgcaac
     7801 tgccctccag ctataccccg ccgcccgcca ccgccctggt gacggcccag cagcccgagg
     7861 cgacgaatcc aaccgcgccg ccggctcccc ctcgcgcggc ggctccggcg ccagccgcaa
     7921 ggtcggccga caatccggcc ggcatccgct tctgatcaga acgccagggt cctgacgccg
     7981 ccgggcgtgc ccagcaggca cacggcggct ttctggtgcg cgaacacgcc gacggtgacc
     8041 acgccgggcc attggttgac ttcgctttcg aagcccagcg gatcggaaat cgcgagaccc
     8101 gcgacatcca ggatgtgctg cccgttgtcg gtcaccagcg gctggccgtc tcgcaggcgg
     8161 actgccgccg tggcgcccat cgcggcgaac tgccgcacca ggcgccgcgt tgccatgggg
     8221 atgatttcca cgggcacggg gaagcggccc agcacttcaa ccagcttgga ctcgtcggcg
     8281 atgcacacga agcgcttcga ttgcgccgca acgatcttct cgcgcgtcaa agcggcgccg
     8341 ccgcccttga tcatgaagcc gtggccatcg atctcgtcgg cgccatcgat gtagaccggg
     8401 atatcgccgg cgtcgttggc gtcgaacacc gcaatgccca gcgcgcggag tctttcggtg
     8461 gaagcattgg agctggacac cgcgcccgcg atgcgatcct tcatcgtggc cagcgcgtcg
     8521 atgaatttgt tgacggtgga gccggtgccc acaccgacga tctcgcccgg taccacatac
     8581 tgcagggccg cccggcccac cagtgttttc agttcgtcct gtgtcacgcg acaatcctgg
     8641 agttttgttg cacttgcgga attatccatg tcattgctgc cctattcgct ggcccgccct
     8701 ttcctgttcg ggctggaccc ggaaaccgcg cacgaactga cgatgcaatc gctggcgcgg
     8761 ctgcagggca cgccgctgga atgggcttat cgcaacggcc tggtcgagga cccggtcgag
     8821 cttgccgggc tgacctttcc caaccgggtc ggcctggccg ccggcctgga caagaacggg
     8881 cgttgcatcg atggcctggg tgcgatggga ttcggcttcg tcgaggtcgg gacggtgacg
     8941 ccgaggccgc agccgggcaa tccgaaaccg cgcatgttcc gcctgccgca ggcgaacgcg
     9001 ctcatcaacc ggctgggctt caacaacgac ggcctggaag ctttcatcac caacgtgaag
     9061 cgctcgtcgt tccggaaaaa gggccgcatc ctggggctga acatcggcaa gaacgcggcc
     9121 acgccgatcg ccaacgccac cggcgactac ctggtctgcc tggacggcgt ctacccgcat
     9181 gcggattacg tgaccatcaa tatttccagc ccgaacaccg ccgacctgcg ctcgctgcag
     9241 ggcgatcaag cgctgggcgg cctgctcggc gcgatagcga agcggcgcga ggagctttcg
     9301 cgcgagcatg ggcggcgtgt tcccatcttc gtgaagatcg cacccgacct ggaccgggca
     9361 caggtcaacg cgatcgccgc agcgctcaag cgccacggca tggacggcgc ggtggccacg
     9421 aacacgacca ccagccggga agccgtccag ggcctgcccc atgcgcagga gacggggggc
     9481 ttgagcggcg cgccgctgct agaggccagc aaccgcgtga tcggccagct gcgttcggcg
     9541 ctgggcaaag gattcccgat catcggtgtc ggaggcgtga tgagcgcgca agacgccgtc
     9601 agcaagatcc gcgcgggtgc cgatgcggtc cagatctaca ccgggctgat ctaccgcggg
     9661 ccgcagctcg taagcgacgc ggcacgcgcg ctcaaggcgc tgtcctgcca gcccacgact
     9721 cagaaagcgt aattgccggc cagcttgagc acgtagccgc gtcccggcgc cgcctcgaaa
     9781 aaccggccgt ttccctcgtt gacgattacc gaaccggcgt agcggcggtc cagcacgttg
     9841 tcgacgcgca catgcgctgc caaggcccag ccccgcaaat cgaagacata ccccgcgtgc
     9901 agcgccagtg tcgtgaacgt tggcgccgcc tcgctgttgg tgtcgttgac atgaacccgg
     9961 ctggagcgcc tcagctccgc gccggcacgc cagccacgcg cgggctgcca tgcgatttcg
    10021 gccgccgaga ccgattgggc agtgcccggg atgcggttgc cagccggtat gaactgcgtc
    10081 ggcacggtgc aagg
//
LOCUS       DASWIY010000474         4341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1843630, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000474 DASWIY010000000
VERSION     DASWIY010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843630"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /gene="nrfH"
                     /locus_tag="VGA59_17015"
     CDS             complement(<1..457)
                     /gene="nrfH"
                     /locus_tag="VGA59_17015"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HEX9721422.1"
                     /translation="MLEQGRTARQRGLAGGTMLLLALAVLIGAAVGIGGYTLVYAEGL
                     SYMSSDPKVCANCHIMQAQFDSWQKSSHHHVAICNDCHLPQGFVGKYVAKALNGYNHS
                     KAYTLQDFDEPIAIKPFNTRILQDNCLACHRALVHEAAMARGRSEEVSCV"
     gene            complement(630..2477)
                     /gene="lpdA"
                     /locus_tag="VGA59_17020"
     CDS             complement(630..2477)
                     /gene="lpdA"
                     /locus_tag="VGA59_17020"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX9721423.1"
                     /translation="MSLVEVKVPDIGDAKDVSVIELLVKPGDAVKAEQSLVTVESDKA
                     SMEIPSTHAGVVKELKVKLGDKVNEGSVLLVLESGTAESPSPKPSPPRGEGAAPVSVP
                     PIPQSGAVPVPPLPQAGEGRGEGLTVPTPSTYTGGADLECDMLVLGGGPGGYSAAFRA
                     ADLGMKVVIVERYATLGGVCLNVGCIPSKALLHVAAVMDEARHFESLGVSFGKPAIDL
                     DKLRAHKAKVVGRLTGGLTAMARMRKVTVVRGVGAFLDAYHLQVEETGGTGQEKTGKK
                     QTVKFGNCIIAAGSQAVQLPFMPKDPRIVDSTGALELPGSPKRMLILGGGIIGLEMGT
                     VYSTLGARLDVVEMLDGLMQGADRDMVRVWQKMNAPRFDKIMLKTKTVGAEATRDGIL
                     VRFEGEQAPKEPQLYDLVLQAVGRSPNGGKIGADKAGVRVTERGFIPVDIQMRTNVPH
                     IFAVGDIVGQPMLEHKAVHEAHVAAEVAAGEKVAFNARVIPNVAYTDPEIAWVGLTED
                     EAKAKGIAIKKGHFPWSASGRAIANTRDEGFTKLLFDAESHRILGGAIVGTHAGDMIG
                     EIALAIEMGADEVDIGKTIHPHPTLGESIGMAAEVAHGTCTDVPPQRKS"
     gene            complement(2474..3064)
                     /locus_tag="VGA59_17025"
     CDS             complement(2474..3064)
                     /locus_tag="VGA59_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9721424.1"
                     /translation="MTTVVVVKKAGQIAIAADTLVTFGDTRLANRFEDNTKIFKIETD
                     AGVSYLGMAGTVAHFPVLRKAIGSMPRELLKLGSRDEVFDTFTKLHPYLKENFFLQTK
                     EEDSDPYESSQFSVVIANANGIFGLYSYREVFEFKEFWGIGSGRSFALGAMHAIYGKT
                     KTAREIAEAGIAAGCEFDRNSAAPFDVFTLKTKDAK"
     gene            complement(3111..>4341)
                     /gene="aceF"
                     /locus_tag="VGA59_17030"
     CDS             complement(3111..>4341)
                     /gene="aceF"
                     /locus_tag="VGA59_17030"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEX9721425.1"
                     /translation="VKPEQSLITVESDKASMEIPSSAGGTVKELKVKLGDKVNVGDLI
                     AVLEGAAADAARDKAPADAAATQPPAPPPAGAQGQAKERRSPTAALPPHEPGMPTGGM
                     PHASPSVRKFARELGVPLEEVKGTGPKGRITQEDVQAFTKAVMSGEASTRAAAAKAPA
                     GGGGEALGLLPWPKVDFARFGPVERKEMGRIRKISGANLHRNWVMIPHVTNHDDADIT
                     ELEAFRVQLNKENEKAGIKVTMLAFLIKACVAALKKFPHFNASLDGDQLVLKQYYHIG
                     FAADTPNGLVVPVLKDADRKGVLAISQEMGELAGKAREGKLSPAEMSGAGFTISSLGG
                     IGGRYFTPIINAPEVAILGVCKSRTEPVWDGKQFQPRLMLPLSLAWDHRVIDGAAAAR
                     FNAYLGTILADFRRTML"
BASE COUNT          683 a         1454 c         1352 g          852 t
ORIGIN      
        1 gcacgcagct gacttcctcg ctgcgccccc gcgccatcgc cgcctcgtgc accagcgcgc
       61 gatggcaggc aaggcagttg tcctgcagga tgcgggtgtt gaacggtttg atggcaatgg
      121 gttcgtcgaa gtcctgcagg gtgtaggcct tcgagtggtt gtagccgttg agcgccttcg
      181 ccacatactt gccgacgaaa ccctgcggca agtggcagtc gttgcagatg gcgacatggt
      241 gatggctgga cttctgccat gagtcgaact gcgcctgcat gatgtggcag ttggcgcaga
      301 ccttggggtc ggagctcatg taggagagcc cttcggcgta cacgagcgtg taaccgccga
      361 ttccgaccgc tgccccgatc agcaccgcta gcgccagcag cagcatcgtg ccgcctgcca
      421 gaccgcgctg tcgcgctgtt cttccctgtt ccaacatggc tctccgcggc gggccaccca
      481 gtcggcgact ggcgtcccat gccgcatcac agaccatgtc taggtcatgc gcgcgctcgt
      541 ttccttgacc ggcgtcaaat tgtgacgagc accggaagaa acgccggggt gggtccgatc
      601 agtcgggctt gacgcccgcc gtcaagacct cagctcttgc gttgcggggg cacgtcggtg
      661 caggtgccgt gggcgacctc ggccgccatg ccgatgctct cgcctagcgt gggatgggga
      721 tggatggtct tgccgatgtc cacttcgtcc gcgcccattt cgatggccag cgcgatctcg
      781 ccgatcatgt cgcccgcatg ggtgccgacg atggcgccgc ccagaatccg atggctctcg
      841 gcatcgaaca gcagtttggt gaaaccttcg tcgcgcgtgt tggcgatggc ccggccggaa
      901 gcgctccacg gaaagtgccc cttcttgatg gcgatgccct ttgccttggc ctcgtcttcc
      961 gtgaggccca cccaggcgat ctcgggatcg gtgtaggcca cgttgggaat gacgcgggcg
     1021 ttgaaagcca ccttctcacc cgcggcgact tccgccgcca catgcgcttc atgaaccgcc
     1081 ttgtgctcca gcatcggctg tcccacgatg tcgccgaccg cgaagatgtg cggcacgttg
     1141 gtgcgcatct ggatatcgac cggaatgaag ccgcgctcgg tgacgcggac acctgccttg
     1201 tcggcaccga tcttgccgcc gttgggactg cggccaacgg cctggagcac gaggtcgtac
     1261 aactgcggct ccttgggggc ctgctcgcct tcgaaccgga ccaggatgcc gtcccgggtg
     1321 gcctccgcac cgacggtctt cgtcttgagc atgatcttgt cgaagcgcgg cgcgttcatt
     1381 ttctgccaga cccgcaccat gtcgcggtcg gcgccctgca tgaggccatc gagcatctcg
     1441 accacgtcga gccgcgcacc cagggtggag tacaccgttc ccatttccag gccgatgatg
     1501 ccgccgccca ggatcagcat gcgcttcggg ctgccgggca gttccagcgc gcccgtggaa
     1561 tccacgattc gcggatcctt gggcatgaac ggcaattgca cggcctgcga tccggcggca
     1621 atgatgcagt tcccgaactt gacggtctgc ttcttgccgg tcttctcctg cccggtaccg
     1681 ccggtttcct cgacctgcag gtggtacgca tccagaaacg cgccgacgcc ccgcaccacc
     1741 gtgaccttgc gcatcctggc catggccgtg aggccgccgg tgagcctgcc caccaccttc
     1801 gccttgtggg cgcgcagctt gtccaggtcg atggcgggct tgccgaagct cacgccgagc
     1861 gattcgaaat gcctcgcctc gtccatcacg gccgccacgt gcagcagcgc cttcgacgga
     1921 atgcaaccga cgttcaggca aacgccgccc agcgtggcat agcgttcgac gatcacgacc
     1981 ttcatgccca ggtccgccgc ccggaatgcg gcggagtagc cgcccggccc gccgccgagc
     2041 accagcatgt cgcactcgag gtcggcgccg ccggtgtagg tggaaggagt tggaacagtc
     2101 agaccctcac ccctgccctc tcccgcttgc gggagagggg gaactgggac cgctcccgat
     2161 tgcgggatag ggggaactga aactggagcc gctccctctc ccctcggggg agagggcttg
     2221 ggtgaggggc tttccgcggt gccgctttcc agcacgagca acaccgaacc ctcgtttacc
     2281 ttgtccccga gcttgacctt cagctccttg accacgccgg catgggtcga cggaatttcc
     2341 atcgatgcct tgtcgctttc cacggtgacc agcgactgct cggccttgac tgcgtccccc
     2401 ggcttgacca gcagttcgat caccgacaca tccttggcat cgccgatgtc cggcaccttg
     2461 acctcgacca ggctcatttg gcgtctttcg tttttagcgt gaacacatcg aagggagcag
     2521 ccgagttccg gtcgaactcg cagcccgcgg cgatgccggc ttcggcgatt tcgcgcgcgg
     2581 tcttggtctt gccgtaaatc gcatgcatgg cgcccagcgc aaagctgcgg cccgagccga
     2641 tgccccagaa ttccttgaat tcgaagacct cgcggtagct gtacaggccg aagatgccgt
     2701 tcgcgttggc gatcaccaca ctgaactggc tcgattcgta aggatcgctg tcctcttcct
     2761 tggtctgcag gaagaaattt tccttgaggt acggatgcag tttcgtgaac gtgtcgaaga
     2821 cctcatcccg gctgcccagc ttgagcagtt cgcgcggcat ggagccgatg gccttgcgca
     2881 gcaccgggaa gtgggccacc gtgccggcca tgcccaggta gctcacgccg gcatcggtct
     2941 cgatcttgaa gatcttggtg ttgtcctcga agcggttggc cagccgtgtg tcgccgaagg
     3001 tcaccaaggt gtccgccgcg atcgcgatct gccccgcttt ttttaccacc acaacagttg
     3061 tcattccgaa cgctccgggt tcgtggtcac cgaagaacgc atcgcgttcc tcacagcatc
     3121 gtgcggcgga aatccgccaa tatggtgccc aggtaggcat tgaaacgggc cgccgccgct
     3181 ccgtcgatga cgcggtggtc ccacgccagc gacagcggca acatcaacct gggctggaac
     3241 tgcttgccgt cccaaaccgg ctccgtccgg cttttgcaga cgccaaggat agccacttcc
     3301 ggcgcgttga tgatcggcgt gaagtacctg ccgccgatgc caccgagcga ggagatcgtg
     3361 aaacccgcgc ccgacatctc ggccggactc agcttgccct cgcgcgcctt cccggccagc
     3421 tcgcccattt cctggctgat cgcaagcacg cccttgcggt cggcgtcctt gaggaccggc
     3481 accaccagcc cgttgggcgt gtccgccgca aaaccgatgt gatagtactg cttgagcacc
     3541 agctggtcgc cgtcgaggga ggcattgaaa tgcggaaact tcttcagcgc cgccacgcag
     3601 gccttgatca gaaaggccag catcgtgacc ttgattccgg ccttttcgtt ctccttgttg
     3661 agctggacgc ggaaggcttc gagttcggtg atgtcggcgt cgtcgtggtt cgtgacgtgc
     3721 ggaatcatca cccagttgcg gtgcagattc gcgccgctga ttttccggat ccggcccatc
     3781 tccttgcgtt cgacggggcc gaacctggcg aaatcaacct tgggccaggg cagcaggccc
     3841 agggcttcgc caccgccgcc ggcgggcgcc ttcgccgccg ccgcccgcgt gctggcttcg
     3901 ccactcatca cggctttggt gaaagcctgc acgtcctcct gcgtgatgcg gcccttgggg
     3961 ccggtgcctt tcacctcctc gagcggcacg ccgagttcgc gcgcgaactt tcgcaccgaa
     4021 ggcgaagcat gcggcatgcc gcccgtgggc atgccgggct catggggcgg caaggcagcc
     4081 gtgggggacc tgcgctcttt cgcctggccc tgcgctccgg ccggaggtgg ggccgggggc
     4141 tgtgtggctg ccgcatccgc gggcgccttg tcccgcgccg cgtcagccgc ggcgccttca
     4201 agcaccgcga tgaggtcccc cacattgacc ttgtcaccca gcttgacctt gagttccttt
     4261 acggtcccgc cggccgacga cggaatttcc atcgaggcct tgtccgattc gacggtgatc
     4321 agcgactgct cgggtttgac g
//
LOCUS       DASWIY010000475         6053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1450657, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000475 DASWIY010000000
VERSION     DASWIY010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450657"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(417..1637)
                     /locus_tag="VGA59_17035"
     CDS             complement(417..1637)
                     /locus_tag="VGA59_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9721426.1"
                     /translation="MHDPLTRGRRLAPGLSTLRLVGALAAVTAALLACGGSDGPDLVA
                     EGKQIFRFDTFGDEAQWTDKLRMHEVISSTVSPVAALAVGLKVDSEALPALVVQGISD
                     GSVDLASPATTVTLLKLDAVVGLKGTVESVGGKDTLTRVGITCALCHSTVDDSFAAGI
                     GKRLDGWPNRDLIPGAIIALSPALTTAQRVAYLSWGKGMYDPRFNIDGKSKPVVIPPA
                     YGLEGIHRITFTGDGNDIAYWNRYVAVTQMGGLGTFTEPRLNLSVANGTVDLVTSKLP
                     ALQAYQLSIKAPAPPAGSFDAAAAARGKVLFEGRGTCATCHTGTTFTDANTMLHPSSD
                     SMAEPETPSYASRSATKLYRTSPLKGVWQHPPYFHDGSAPTLESVVQTYNTKRSLGLT
                     PQEIADLAQYLRSL"
     gene            complement(1768..1959)
                     /locus_tag="VGA59_17040"
     CDS             complement(1768..1959)
                     /locus_tag="VGA59_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEX9721427.1"
                     /translation="MPDDKSKSHGQDRERINVNQDYELRDWAKSLNTTPERVKEAVQA
                     VGDRADKVREFLKERESRG"
     gene            complement(2056..2790)
                     /locus_tag="VGA59_17045"
     CDS             complement(2056..2790)
                     /locus_tag="VGA59_17045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9721428.1"
                     /translation="MIEKHLDIPSADGDMNTFVVHPEEGGPHPLVLFYMDAPGKREEL
                     HDMARRFAAVGYYVVLPNLYYRKTREFVVDRASEASMAAMFELMGHLTNALVVSDTEA
                     MLRFADTQPEADAACVGAVGYCMSGPFVMAAAASFPDRFRSIASIHGANLVTDREDSA
                     HLMAPRIHCSSYFACAEIDKWASKATVDKLQAALLEAGTPHRIEWYPGAEHGFVFPQR
                     ACYDKTSAERHWERLFDLFRRTLDRK"
     gene            2954..4726
                     /locus_tag="VGA59_17050"
     CDS             2954..4726
                     /locus_tag="VGA59_17050"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX9721429.1"
                     /translation="MKFSTRGFLDWGLHALVLMLLTAFGWAAAATEPSRVWPESKIAA
                     EYWLDGSGKASLEAAHAQFEAKRGLAADPRQIMPLGGGRAVWYRLQLPGVAAPTPAVF
                     SVPYPGMDNVELFRPDGKGGWRAERAGDSRPVNEWPIRYLYPAFSLTVQPAELQATYL
                     RVQHSHPIGITLELWDAGGFAEAGKIWHLILGAYIGFMALVILLSVTNAVSWRDPIHL
                     YYALHVVFVGLSLMSLNGLAGEYLWPGNAWWNDIAAVALPSAALAGMGLFVRELAAER
                     GRLLVSRLLLAHVAISLLMMLSIILFGRGNRFVAFNVYGVLGLALMLGVMVWYSLRRP
                     QAGLFVLAGLAALTAGFMFPVMRNIGLLPITFGIQYAPQIGGALEIPLVLIGLYLRSR
                     ERRDNRLRLDALSRSDPLTGLANHRVLVDRLQLLLRRAGGDAAIGAVMRVHVANLSAI
                     RNEHGREAAEAALVRAAECVARAANEGDTVAREQGGDLVLLLEGRATREQIAYAGRDI
                     IARGLKFSGLLPPGVMLTLHVGVMGAPLPEGNATKLLAMLADVIREIGNDPHGRALRI
                     VGAADPSAVAQGGRDMALSAGSGI"
     gene            4824..5489
                     /locus_tag="VGA59_17055"
     CDS             4824..5489
                     /locus_tag="VGA59_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721430.1"
                     /translation="MGFFARLFGGSRSSAGDSRLYSQLGTGGSKGPLASENATRRELL
                     RVALSETLLRQGIPTSWITGEMLTATSRDRASGIHWRLLVKHWEPKLMLHLVALQNRL
                     IGRVQMFDPMAETWLMGISWQLALRDESACPALPHPGSWTAEPRPARAPLPPRTVPGG
                     SGSVIAGPVHIQTGTNDVGSDLERLMVIREADVRAQENAEAAGTDHEATRPMYLKTEP
                     DKL"
     gene            complement(5500..5775)
                     /locus_tag="VGA59_17060"
                     /pseudo
     CDS             complement(5500..5775)
                     /locus_tag="VGA59_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
     gene            5869..>6053
                     /locus_tag="VGA59_17065"
     CDS             5869..>6053
                     /locus_tag="VGA59_17065"
                     /inference="COORDINATES: protein motif:HMM:NF023331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721431.1"
                     /translation="MHRSLAQQIEHWVKLGIGVERSAGATVDEVRAAALRYRHVRDEA
                     AVRSGRRTASSLHVIPAS"
BASE COUNT          998 a         1943 c         2014 g         1098 t
ORIGIN      
        1 gttcgggcgc ggacggtgtg tgtatcgtgc gcggcctggg cgacgagccg cggaaggtgg
       61 tgccggcact ccaggctgct ctcgcaggcg ggcgcaggga attcatgctt cgtggaactc
      121 cattgacgtg gccgcacccg agcctgcccc ttcaggagtg atccggcgcc gagcatcgcc
      181 gcaacggagg cccgatccgg gatccatgac ttcgtggatt gcgggtcgag cccgcaatga
      241 caagaggctg tccatgacac aagaggctgt catcccggac tcgatccggg atccatgact
      301 tcgtggattg cgggtcgggc ccgcaatgac aagtggcgcc gtcaagcccg caatgacaag
      361 aggcgccgtc aagcccgcaa tgacaagagg cgctgtcgag cccgcaatga caagttctag
      421 agcgacctca aatactgcgc cagatcggcg atctcctgcg gcgtcaggcc cagcgagcgc
      481 ttggtgttgt acgtctgcac cacgctttcc agagtcggcg cggagccgtc gtgaaagtac
      541 ggcgggtgct gccacacgcc tttgagcggt gacgtgcgat acagcttggt cgccgaccgt
      601 gaggcatagc tcggcgtttc gggctccgcc atcgaatcgc tcgacggatg cagcatcgtg
      661 ttggcgtcgg tgaaggtcgt cccggtatgg caggtcgcgc aggtgcccct gccctcgaac
      721 agcaccttgc cgcgtgcggc cgcggcggcg tcgaagctgc ccgccggcgg cgccggtgcc
      781 ttgatcgaaa gctggtaggc ctgcagcgcg ggcagcttgc tcgtgaccag gtcgacggtg
      841 ccgttggcaa cgctcaggtt caggcgcggc tcggtgaacg tgcccaggcc gcccatctgc
      901 gtcaccgcca cgtagcggtt ccagtaagcg atgtcgttgc cgtcaccggt gaaggtgatg
      961 cggtggatgc cttccagccc gtaggccggc ggaatcacca ccggcttgct cttgccgtcg
     1021 atgttgaagc gcgggtcgta catgcccttg ccccacgaaa ggtaagccac cctctgcgcc
     1081 gtcgtcagtg ccggcgacag cgcgatgatg gcgccgggaa tcaggtcgcg gttgggccag
     1141 ccgtcgagcc gcttgccgat gcccgccgcg aacgagtcgt ccaccgtcga gtggcaaagt
     1201 gcgcaggtga tgcccacgcg ggtgagcgtg tccttgccgc cgacggactc gacggttccc
     1261 ttcaggccga ccaccgcgtc cagcttcagc agcgtcaccg tcgtggccgg gctggccaga
     1321 tcgacgctgc catcgctgat gccttggacc accagtgccg gcaaggcttc cgaatcgacc
     1381 ttcaggccga ccgccagcgc cgcgacgggg gacaccgtcg agctgatcac ttcgtgcatc
     1441 cgcagcttgt cggtccactg ggcctcgtcg ccgaaggtgt cgaagcgaaa gatctgcttg
     1501 ccttcggcga ccaggtccgg cccgtccgaa ccgccacatg ccagcagcgc ggcggtcacg
     1561 gcggccagcg ctcccacgag ccgcagcgtt gacagtccgg gcgccagccg gcgcccgcgg
     1621 gtgagtggat cgtgcatcgc gatgtccttt cgttttccag gttggatgcc gttagatgcg
     1681 ccccaccggg gaaaggtgac aagcgcgaca atgaatctcc ggcagagcaa cgggcgcacg
     1741 gccgcgccgg gccatgccgg cggtgcgtca gcctctgctt tcgcgttctt tcaggaattc
     1801 acgcaccttg tcggcacggt cgccaacggc ctgcacggct tccttcacgc gctcgggcgt
     1861 ggtgttcagg ctcttggccc agtcgcgcag ctcatagtcc tggttgacgt tgatgcgctc
     1921 gcggtcctgg ccatgggact tgctcttgtc gtcgggcatg gatgtctcct gttcggttgg
     1981 aaagggaccc atgctagaaa cacagccggc ccggcgcatg taggacgctg cctgaaaact
     2041 catgcccggc aaccgctatt tgcggtcgag cgtgcgcctg aacaggtcga acagccgctc
     2101 ccagtgccgc tcggcgctgg tcttgtcgta gcaggcgcgc tggggaaaca cgaagccgtg
     2161 ttcggcgccg ggataccact cgatgcggtg cggcgtgccg gcctcgagca gtgctgcctg
     2221 cagcttgtcc accgtggcct tggacgccca cttgtcgatc tcggcgcagg cgaagtagct
     2281 cgagcaatgg atgcgtggcg ccatcaggtg ggccgagtcc tcgcggtcgg tcaccaggtt
     2341 ggcaccgtgg atggacgcga tgctcctgaa gcggtcagga aagctggctg ccgccgccat
     2401 cacgaacggg ccgctcatgc aatagcccac cgcgcccacg cacgccgcgt cggcttcggg
     2461 ctgggtatcc gcgaagcgca gcatcgcctc ggtgtcgctc accaccagcg cattggtcag
     2521 gtggcccatc agctcgaaca tcgcggccat gctggcctcg ctggcgcggt ccaccacgaa
     2581 ctcgcgcgtc ttgcggtaat agaggttggg cagcaccacg taatagccca cggccgcgaa
     2641 gcgccgggcc atgtcgtgca gttcttcgcg cttgcccggt gcgtccatgt agaacagcac
     2701 gagcggatgc ggcccgcctt cctcgggatg cacgacgaaa gtgttcatgt caccgtcggc
     2761 agaggggatg tcgagatgtt tttcgatcat ggcgtgggct ccggacaact tcgcgcattg
     2821 tttactgcat tcagatgata ggttccgcgt caaatgccgc gtacattgga cgcgccattg
     2881 aaatgaccgc ccaagatcaa tcggcagaag gccaagccat gcaaccaaca acggcgggat
     2941 cgaagacttg gatttgaaat tcagtacgcg gggattcctg gactggggtc tgcacgcgct
     3001 cgtgttgatg ctgttgaccg ctttcggctg ggccgccgcg gcgaccgagc cctcccgcgt
     3061 gtggccggag agcaaaatcg ccgccgaata ctggctggat ggcagcggca aagcctcgct
     3121 ggaagcagcg catgcgcaat tcgaggccaa gcgtggtctg gcggcggatc cgcgccaaat
     3181 catgccgctg ggcggaggac gagccgtttg gtaccggctc cagcttccag gagtcgcagc
     3241 gccaacgccc gcggtgttca gcgtgccata tcccggcatg gacaatgtgg aactgttccg
     3301 acctgacggc aagggagggt ggcgcgccga gcgtgccggc gactcacggc cggtgaacga
     3361 atggccgatc cgctacctgt atccagcctt cagcttgacg gtgcagccgg cggaattgca
     3421 ggccacatac cttcgcgtgc agcacagcca tcccatcggg atcaccctgg agctttggga
     3481 cgccggcggc ttcgccgaag ccggaaagat ctggcacttg atcctgggcg catacatcgg
     3541 gttcatggcg ctggtgatcc tgctcagcgt gacaaatgcg gtgtcgtggc gcgaccctat
     3601 acatctttat tacgcattgc acgtcgtctt cgtcgggctc agcctcatgt ccctgaacgg
     3661 gctcgcgggc gaatacctct ggcccggcaa cgcgtggtgg aacgacatcg cggcggtggc
     3721 gcttccgtcc gcggccctgg ccggcatggg cctcttcgtg cgcgagctgg ccgccgagcg
     3781 cggccgcctc ctggtgtcca ggctcctgct ggcgcatgtc gccatctcgt tgctcatgat
     3841 gctgagcatc attcttttcg ggcgcggcaa caggttcgtg gcgttcaacg tctacggcgt
     3901 cctgggcctg gccctgatgc tcggagtgat ggtgtggtat tcgctgcgcc gaccgcaggc
     3961 aggattgttc gttctcgccg gactcgctgc cctgacggcg ggcttcatgt tccccgtgat
     4021 gcgaaacatc gggctgcttc ccatcacttt cggcatccag tacgcgccgc agatcggcgg
     4081 tgccctggag atcccgctgg tgttgatcgg cctgtatctt cgcagccgcg agcggcgcga
     4141 caaccgcctg aggctcgacg cgctttcccg cagcgacccg ctgaccgggc tcgccaacca
     4201 tcgtgtcctg gtcgaccggc tgcaactcct cctgcggcgc gcgggaggcg atgcggcgat
     4261 cggtgccgtg atgcgggtcc acgtggccaa cctgtccgcg atccgaaacg agcatggacg
     4321 ggaagcggcc gaagcggcac tggttcgggc cgcggagtgc gttgccaggg cggccaacga
     4381 gggagatacg gtggcgcggg agcagggcgg agacctcgtg ctgctcctgg aaggccgcgc
     4441 gacgcgcgag cagatcgctt acgccggccg cgacatcatc gcgcgagggc tcaagttttc
     4501 cggcctgctg ccgccggggg tgatgctcac gttgcacgta ggcgtgatgg gggcgcctct
     4561 tccggaaggc aacgccacaa agttgcttgc catgctggcc gatgtgatcc gagagatagg
     4621 caacgacccg catggccggg cgctgcggat cgtgggcgcc gcggacccgt cggccgtcgc
     4681 ccaaggcggc agggacatgg cgctttcggc cggcagcgga atctgaaatt cggacaaatc
     4741 tggagctcgc cagcaagaat tgccgccttt cgatacgatg ctgggcataa agctggcggt
     4801 tctcgcacag gagggagttc accatgggtt tttttgcaag gctgttcggt ggctcgcgga
     4861 gttcggcggg cgattcgagg ctctattcgc agctgggcac cggcggctcc aaaggtcccc
     4921 tcgcgtccga gaatgccaca cgccgtgaac tgctgcgcgt cgccttgagc gaaacgctgc
     4981 tgcgtcaggg cattcccacc tcctggatca ccggggagat gctcacggca acgtcgcgtg
     5041 accgcgcatc cggcatccac tggcggctgc ttgtcaagca ctgggaacca aaactcatgc
     5101 tccatctggt cgccttgcag aacaggctga tcggccgcgt gcagatgttc gatccgatgg
     5161 ccgaaacctg gctcatgggg atttcctggc aattggcgtt gcgtgacgaa tcggcgtgcc
     5221 ccgccctgcc gcaccctggg tcatggaccg ccgaacctcg tcctgcccgc gctccattgc
     5281 ctccacggac ggtgccgggc ggatcgggaa gcgtcatcgc cgggccggtt cacatccaaa
     5341 ccggcacgaa cgacgtcggg tcggatctcg agcggctgat ggtcatccgc gaggccgacg
     5401 tgcgcgccca ggagaacgcc gaggctgcgg ggaccgatca cgaggcgacg cggccgatgt
     5461 acttgaaaac ggaacccgac aagctgtagc gggcgcccgt cagccggctt tccggatcgt
     5521 cccctgcgcg gcggcgcaca gcttgcgctc gccacccagc tccacgaaga tatcgcagcg
     5581 gcacacggct tgtgtcttgc cgctgcctac cacggtggcc acagccacca aacggtcgcc
     5641 atccttggcg ggccgcatgt agttgatctt gaattccgaa gtgagcacct tgcccagcac
     5701 cgagccgccg gcgtaggtga gggcgttgtc ggccaggtag gccagcacgc cgccgtgcac
     5761 gaatccgtcc tgttgcttgc gctattcctt gccttgtcat cccgggcttg acccgggatc
     5821 catggattgc gggtcaggcc cgcaatgaca atgcaagagg ctgctctcat gcatcgaagc
     5881 cttgcccagc agattgaaca ctgggtaaag ctgggcattg gcgtcgagcg atccgcgggc
     5941 gccacggtgg acgaggtacg cgcagcggca ctgcggtatc gccatgttcg agacgaagcg
     6001 gccgtccgat cgggtcgaag gaccgcttcc agccttcatg tcattcccgc ctc
//
LOCUS       DASWIY010000476         8360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2936006, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000476 DASWIY010000000
VERSION     DASWIY010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8360
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2936006"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..711
                     /locus_tag="VGA59_17070"
     CDS             151..711
                     /locus_tag="VGA59_17070"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX9721432.1"
                     /translation="MKLKDFAADSHDVARTLIGATLLVDGVGGRIVETEAYDRHDPAS
                     HSFSGPTPRNAVMFGPPGHAYVYRSYGIHWCLNFVCREEGHGAGVLIRAIEPLQGIEL
                     MRGRRGLQDVRLLCSGPGRVGQALGITRDLNGVRLDRPPFKVLAPPEAQKIVAGPRIG
                     ISKAIDVPWRFGLAGSRFLSRAFRTS"
     gene            complement(713..865)
                     /locus_tag="VGA59_17075"
                     /pseudo
     CDS             complement(713..865)
                     /locus_tag="VGA59_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761449.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
     gene            complement(862..1104)
                     /locus_tag="VGA59_17080"
     CDS             complement(862..1104)
                     /locus_tag="VGA59_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006753129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9721433.1"
                     /translation="MTTATVTSKGQITIPANVRQALQVEPGDRLEFVEVDPGRFEVVA
                     ATHSVTELKGMFGKAARRVSIEDMNKAIAARGAAAR"
     gene            complement(1257..2552)
                     /locus_tag="VGA59_17085"
     CDS             complement(1257..2552)
                     /locus_tag="VGA59_17085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX9721434.1"
                     /translation="MGIHIIKIPDIGEGIAEVELVAWHVKPGDQVVEDQGLADVMTDK
                     ATVEIPSSVAGKVLALGGEIGSLMAVGSELIRIEVEGTPGRQAKPAAPVQAASPVAAA
                     PMPAPAAAPPQVQARAAAEPATTTAAPAPPELRPHDDKPIASPSVRRRAWELGIDLKN
                     VRARGTAGRIMHADLDAYVASGAGAVRPATARAALGARRDDEEKIPVIGLRRKIAQKM
                     QEAKRHIPHFAYVEEVDVTELEALRARLNAKLQGQRPRLTLLPFLMRAVASAVREFPQ
                     VNAHFDDTAGVVTRYGAVHIGVATQTNAGLMVPVVRQAQALDLWDSAAEVARVAEAAR
                     KGRATRDELTGSTITISSLGALGGIVSTPIINAPEVAIIGVNRIVLRPMMLGGAIVPR
                     QMMNLSSSFDHRVVDGQHAAEFVQALRGLLECPATLFVE"
     gene            complement(2555..3559)
                     /locus_tag="VGA59_17090"
     CDS             complement(2555..3559)
                     /locus_tag="VGA59_17090"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX9721435.1"
                     /translation="MTMIQALRSAMDVMLERDPNVVVFGQDVGYFGGVFRVTEGLQAK
                     YGKSRVFDAPISEGGIVGSAIGMAAYGLRPVIEVQFADYFYPASDQIVSEAARMRHRS
                     AGDFTAPITIRMPCGGGIFGGQTHSQSPEALFTHVCGLRTVMPSNPYDAKGLLIACIE
                     NDDPVIFLEPKRLYNGPFDGHPDRPMVPWSAHPLGVVPDGYYTVPLESASIFRPGEQL
                     TVITYGTMVFVSEAAARETGLDAEIIDLRSLWPMDLQTLIDSVKKTGRCVVVHEATRT
                     SGFGAELTALVQEHCFYHLEAPIERVAGWDTPYPHAQEWAYFPGPDRVAAAMRRVMED
                     "
     gene            complement(3610..4854)
                     /locus_tag="VGA59_17095"
     CDS             complement(3610..4854)
                     /locus_tag="VGA59_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HEX9721436.1"
                     /translation="MSQHKPLRLHVPEPSGRPGHETDFSYLHLSPAGAVRRPPVDASP
                     AATSDLAFSLVRVLDDDGRAVGPWAPQVDPQLLRTGLRAMLKTRAYDARMLIAQRQKK
                     ISFYMQCLGEEAIATAHALSLQPGDMCFPTYRQQGLLLARDDVSMVEMICQLMSNERD
                     PLKGRQLPVMYSYKRAGFFTISGNLATQFIQAVGWGMASAIKGDTKIASAWIGDGATA
                     EADFHTALTFAHVYRAPVILNVVNNQWAISTFQAIAGGEATTFAARGVGCGIASLRVD
                     GNDFLAVYAASRWAAERARSNLGPTLIEWITYRAGAHSTSDDPSRYRPADDWAHFPLG
                     DPIARLKQHLVLLGAWSDQEHEATQKELEGQVLAALKEAERYGSLADGHVAGAATMFE
                     DVYKDMPEHLRRQRAELLGAGS"
     gene            5163..5429
                     /locus_tag="VGA59_17100"
     CDS             5163..5429
                     /locus_tag="VGA59_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721437.1"
                     /translation="MERISGPYKGYYIAAYTVAAGDQFVGYAKVCAEEPDSVWNPQPV
                     EKLTSAPGCRTELEAVLAAEQKARQAIADILGNWDPITAPGELN"
     gene            complement(5446..5940)
                     /locus_tag="VGA59_17105"
     CDS             complement(5446..5940)
                     /locus_tag="VGA59_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721438.1"
                     /translation="MKTRHALLGLLLGVAAGQALACYTVFDRVGRVVYSDPSPPVDMS
                     RPLHETLPARFPGGHMVFDTQVNCASVAPPPPAVAAREGAPLLTDRRTARAMNVPYTV
                     MQGAIVMVESRHARLAPGVTVVPVEAVALSSPGRGTVITELREPPMTIVQSGDGVVIS
                     ELRQ"
     gene            complement(6092..6727)
                     /locus_tag="VGA59_17110"
     CDS             complement(6092..6727)
                     /locus_tag="VGA59_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEX9721439.1"
                     /translation="MRWTQEQMAARAAQELQDGFYVNLGIGIPTLVANYVGDKEVWLQ
                     SENGMLGIGPFPTEEEVDADLINAGKQTVTTIPGSSIFGSHDSFAMIRGGKINLSILG
                     AMQVSEKGDLANWMIPGKMVKGMGGAMDLVAGVKRTIVLMEHVARKKDGTEDLKILPK
                     CTLPLTGVGVVDRIITDLAVMDVTPQGLKVIEMAPGVTMEVLQSKTGVKLH"
     gene            complement(6818..7519)
                     /locus_tag="VGA59_17115"
     CDS             complement(6818..7519)
                     /locus_tag="VGA59_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEX9721440.1"
                     /translation="MNKLFPSAAAALQGVVRDGQLLAVGGFGLCGIPETLIEALRDSG
                     VKDLTVISNNAGVDGFGLGKLLESRQIRKMISSYVGENKEFERQYLAGELELEFTPQG
                     TLAEKLRAGGAGIPAFFTKTGVGTIVADGKELREFDGETYVMERALVPEVALVKAHVA
                     DKSGNLRFRLTARNFNPAAAMAGKICIVEVEHLVETGELLPDDIHLPGIYVHRIVVNP
                     HPEKRIEKRTLSESK"
     gene            7618..8163
                     /locus_tag="VGA59_17120"
     CDS             7618..8163
                     /locus_tag="VGA59_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9721441.1"
                     /translation="MDVDYRLAFNLAPVGLCLSRNRSIVDCNQQLCDMFGASRELLIG
                     QSFQVLYPSADEYERLGARMAPILNAKGHYADDRIMKRANGEVFWCHVTGRALNREAP
                     HEAGIWAFEDLSSQRPVKAELTAREREVAARLLDGMTSKEIGKALGISHRTVEIYRAR
                     LMRKYKASTTADLVHKLMVGE"
     gene            complement(8165..>8360)
                     /locus_tag="VGA59_17125"
     CDS             complement(8165..>8360)
                     /locus_tag="VGA59_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9721442.1"
                     /translation="SLVTVAMLTALGKQHELKAHVRAALNNGATVKELQELLLHATIY
                     CGVPAAVDAFRIAAEVIDGK"
BASE COUNT         1436 a         2780 c         2697 g         1447 t
ORIGIN      
        1 gcgtgtcgaa gtcaaactga agatcaaacc cgccgcgcgc agcgggcacg ccgtaccagc
       61 ttgatgcgct accaggcaag gtgagaggta tcgcggcatg cgcaagacat aacagcgtaa
      121 aaatccttta ccattaaaac ggtttgccgc gtgaagttaa aggatttcgc cgccgattct
      181 catgacgtcg cacgcaccct gatcggcgcc acattgctgg tcgatggcgt cggcgggcgc
      241 atcgtggaaa ccgaggcata cgatcgccat gaccccgcgt cgcacagctt ttccggtccc
      301 acgccgcgca acgccgtgat gttcggcccg cccggccatg cgtacgtcta ccgctcctac
      361 gggatccact ggtgcctgaa tttcgtctgc cgtgaagaag gccacggcgc cggggtgctg
      421 atccgcgcca tcgagccgct gcaaggcatc gagctcatgc gcgggcggcg cggcctgcag
      481 gatgtgcgcc tgctgtgctc gggtccgggc cgcgtcggcc aggcacttgg catcacgcgc
      541 gatctcaacg gcgtccggct ggaccggccg ccgttcaagg tgctggcgcc gccagaagcg
      601 cagaaaatcg ttgccggccc gcgcatcggc atcagcaagg cgattgacgt gccgtggcgg
      661 ttcggcctgg ccggctcgcg ctttctcagc cgggcgttcc ggacaagtta gttagcaccc
      721 agcccaactc gacgaccgaa acgagagata cgaatccggg atcatcgacc gtgagcgact
      781 ccatcagctt cgccgccttc agcgactgcg tggcatcgtc ctgcatgatg tagcgcacca
      841 gcacgttggt gtccaagcca atcatcgtgc tgccgcgccg cgcgccgcga tcgctttgtt
      901 catgtcctca atcgacacgc gccttgccgc cttgccgaac atgcccttca gttccgtgac
      961 cgaatgcgtt gcggcgacga cctcgaaccg gcccgggtcg acctcgacga actccagccg
     1021 atcgcctggc tcgacttgca aagcctggcg gacgttggcc gggatcgtga tctggccctt
     1081 gctggtgacg gtggcggtgg tcatggcata aactcctcac aatgccttac tttatagtaa
     1141 ggcgcgcgcg cagtcgactc cccagcggca tggcccgggg gcggtagctg ggatcaacct
     1201 ccgttcaggc ttgccttggg cttggacccc cgcatctcat cccgcaaagt cggcccctac
     1261 tccacgaaca gcgtggccgg gcactccagc aggccacgca gggcctgcac gaactccgcc
     1321 gcatgctggc cgtccaccac gcggtgatcg aacgaagaag acagattcat catttgccgc
     1381 ggcacgatgg cgccgccgag catcatcggg cgcagcacga tgcggttgac accgatgatc
     1441 gccacttccg gcgcgttgat gatgggcgtg gagacgatgc cgcccaaagc gcccaggctg
     1501 ctgatcgtga tggtcgatcc cgtcagttcg tcgcgcgtgg ccctgccctt gcgtgcggcc
     1561 tcggccaccc gcgccacctc ggccgcgctg tcccagagat cgagggcctg ggcctgccgc
     1621 accaccggaa ccatcaagcc ggcgttcgtt tgcgtggcga caccgatatg cacggcgccg
     1681 tagcgcgtca cgacaccggc cgtgtcgtcg aaatgggcgt tcacctgcgg gaattcgcgc
     1741 acggccgatg cgacggcgcg catcaggaag ggcagcaacg tcagacgtgg ccgctgaccc
     1801 tgcagcttcg cgttcaggcg ggcccgcagc gcctccagct cggtgacgtc cacttcctcg
     1861 acataggcga agtgcggaat atgccgcttg gcctcctgca tcttctgcgc gatcttccgg
     1921 cgcaggccga tcacggggat tttttcttca tcgtcacggc gcgcacccag ggcggcgcgc
     1981 gcggtcgccg gacgcaccgc ccctgcccca ctcgcgacat aggcatccag gtcggcatgc
     2041 atgatccggc cggccgtccc cctggcccgt acattcttca ggtcgatgcc gagctcccag
     2101 gcgcggcggc gcaccgaagg cgaagcgatg ggcttgtcgt cgtggggacg caattccggc
     2161 ggcgccgggg cggcggtcgt ggttgcgggc tcggccgcag cgcgagcctg aacctggggt
     2221 ggggctgcgg caggagcagg catgggcgcc gcggccactg gcgaagccgc ctggaccggc
     2281 gcggccggct tcgcctgccg gccgggcgtc ccttccacct cgatgcggat cagctccgac
     2341 cccacggcca tcaagctgcc gatctcgccg cccagcgcca gcaccttgcc ggcgacggac
     2401 gacggaatct ccaccgtggc cttgtcggtc atgacatcgg ccaggccctg gtcttcaacc
     2461 acctgatcgc caggcttgac atgccaggcc accagctcga cctcggcgat gccttcgccg
     2521 atgtcgggaa tcttgatgat atgaatcccc actgtcagtc ctccatcacg cggcgcatcg
     2581 cggcggcgac gcgatccggg ccggggaaat aggcccattc ctgcgcatgg ggataaggcg
     2641 tgtcccagcc ggccacgcgc tcgatcggcg cttcgaggtg atagaagcag tgctcctgca
     2701 ccagcgccgt cagctcggcg ccgaagccgc tggtgcgcgt ggcctcgtgc acgaccacgc
     2761 agcggcccgt cttcttcacc gaatcgatca gcgtctgcaa gtccatcggc cacaagctgc
     2821 gcaggtcgat gatctcggcg tccaggccgg tctcgcgcgc cgccgcctcc gagacgaaga
     2881 ccatcgtgcc gtaggtgatc accgtcagct gctcgccggg gcggaatatc gacgccgact
     2941 ccagcggcac ggtgtagtag ccgtcgggca ctacccccaa cgggtgcgcc gaccacggca
     3001 ccatgggccg gtcgggatgg ccgtcgaaag ggccgttgta caggcgcttg ggttccagga
     3061 agatcaccgg atcgtcgttc tcgatgcacg cgatcagcag gcctttggcg tcataggggt
     3121 tgctcggcat cacggtgcgc aagccgcaga catgggtgaa cagggcttcg gggctctggc
     3181 tgtgcgtctg cccgccgaag atgcctccgc cgcagggcat gcggatggtg atgggcgcgg
     3241 tgaaatcgcc cgcggaacgg tggcgcatcc gcgcggcctc ggagacgatc tggtcggagg
     3301 ccgggtagaa ataatcggcg aactgcactt cgatgaccgg ccgcaagcca taggcggcca
     3361 tgccgatggc cgagccgacg atgccccctt cggaaatggg cgcatcgaac acgcgcgatt
     3421 tgccgtattt ggcctgcagg ccctcggtaa cgcggaacac gccgccgaaa tagcccacgt
     3481 cctggccgaa gacgacgaca ttgggatcgc gctcgagcat cacgtccatc gcggaacgca
     3541 gcgcctggat catcgtcatc cggctgctgg ccgtgccggc ggtcgccaac tgcgcctggt
     3601 cccgcgcgtt cacgatccgg ctcccagcag ttccgcgcgc tggcgccgca ggtgctccgg
     3661 catgtccttg tagacatcct cgaacatcgt tgcggcaccg gccacgtggc catcggccag
     3721 ggagccgtag cgctcggcct ctttcagcgc ggcgagcacc tgcccttcga gctctttctg
     3781 ggtggcttca tgctcctggt cggaccaggc gcccaggagc accaggtgct gcttcaggcg
     3841 cgcgatcggg tcgcccagcg ggaagtgcgc ccaatcgtcc gccggccggt accgggacgg
     3901 gtcgtccgag gtggagtgcg cgccggcgcg atatgtgatc cactcgatca gcgtcggccc
     3961 caggttgctg cgcgcgcgct cggccgccca gcgcgaggcg gcatagaccg cgaggaaatc
     4021 gttaccgtcc acgcgcaggg aggcgatccc gcagccgaca ccgcgggcgg cgaacgtggt
     4081 cgcttcaccg ccggcgatgg cctggaaggt cgagatcgcc cactggttgt tcaccacgtt
     4141 caggatcacg ggcgcacggt acacatgggc gaaggtcagc gcggtgtgaa agtccgcttc
     4201 ggcggtggcg ccgtcgccga tccaggccga tgcgatcttg gtatcgccct tgatcgccga
     4261 ggccatgccc caacccactg cctggatgaa ctgcgtggcc aggttcccgg agatggtgaa
     4321 gaagccggcg cgcttgtacg aatacatcac ggggagctgc cggcccttga gcgggtcgcg
     4381 ctcgttcgac atgagctggc agatcatctc gaccatcgag acatcgtcgc gcgccagcag
     4441 caggccttgc tggcgatagg tgggaaagca catgtcgccg ggctgcagcg acagcgcgtg
     4501 cgcggtggcg atggcctcct caccaaggca ttgcatgtag aacgagatct tcttttgccg
     4561 ctgagcgatc agcatccgcg catcgtaagc gcgggtcttc agcatggctc gcaggccggt
     4621 gcgcagcagc tgcgggtcca cttgcggggc ccacggaccc acggcccggc catcgtcgtc
     4681 cagcacgcga accaggctga acgcgaggtc gcttgtcgcg gccggcgacg cgtccacggg
     4741 cgggcgcctg actgcgcccg cgggcgacag gtgcagatag gaaaaatcgg tttcgtggcc
     4801 cggccgcccc gagggctcag gtacgtgcaa tcgaaggggc ttgtgttggc tcatccgcgt
     4861 taccaggaga aacatcctac cgctgaggcg cgacggacgc aaacctcagt ttgcgcgcac
     4921 tcggaccgtt cgcggccgcc acgccggcac ggctgcgctg cttgtcgaga tgcttttcat
     4981 aaaaacacgc tccctgtgca tcgatccaat ctaggcatgc gggccatcgc cggccgtcgg
     5041 acaatgcgga cgatgggtaa cgaaagcgtt gcaatcgggg gcggccggcg ctcgtttgta
     5101 tctcgtgttt cgaattgctt ccgaatgaga aagatcagtt gccccagcgt agcattgccc
     5161 ggatggaacg catcagcggt ccttacaagg gctattacat cgcggcctac acggtagcgg
     5221 cgggcgacca attcgtcggc tacgccaagg tgtgcgcgga agagcccgac agtgtctgga
     5281 acccgcagcc ggtggagaaa ctgaccagcg cgccgggctg caggaccgag ctggaagccg
     5341 tcctggccgc ggagcagaaa gcaaggcagg ccattgccga catcctcggc aactgggacc
     5401 cgatcaccgc gccgggcgag ctgaactgag ccctgcggct cggcgtcact ggcgcagttc
     5461 gctgatcacg acaccgtcgc cggactgcac gatcgtcatg ggcggttcgc gcaattcggt
     5521 gatgaccgtc ccacgacccg gcgacgagag cgcgaccgct tccacgggaa caacggtcac
     5581 tccaggtgcg agccgcgcat ggcgtgactc aaccatgacg atggcacctt gcatcaccgt
     5641 gtagggaacg ttcatcgccc gggccgtgcg gcggtcggtc agcaggggcg caccctcgcg
     5701 tgccgcgaca gcgggtggcg ggggagcgac cgaggcgcag ttgacttgcg tatcgaacac
     5761 catatggccg cccgggaacc gcgccggcag ggtttcatgc agcgggcggc tcatgtcgac
     5821 cggcggtgac gggtcgctat agacaactcg cccgacccgg tcaaagaccg tgtagcaagc
     5881 cagcgcctgg cccgcggcca cacccagcag caaacccagc agtgcgtgtc ttgtcttcat
     5941 gatttgcctc cagcacggcg tgcgtgcgat tgatgggcaa tcattggcac gcggccccgc
     6001 ggcgcgcgca gtcaaaacca gtcttttgtt gtcagcgggc accgacgccg cgttgcagcg
     6061 catcgggccg ggcgccaacg ccgaagccgc ctcagtgcag cttgacaccc gtcttggact
     6121 gcagcacttc catggtcacg cccggggcca tctcgatgac cttcaacccc tgcggcgtca
     6181 catccatcac ggccaggtcg gtgatgatgc ggtcgaccac gcccacgccc gtcagcggca
     6241 gggtgcactt cggcaggatt ttcaggtcct cggtgccgtc cttcttcctg gccacatgct
     6301 ccatcagcac gatggtgcgc ttcacgccgg ccaccaggtc catggcgccg cccatgccct
     6361 tgaccatctt gccggggatc atccagttgg ccaggtcgcc cttctcgctg acctgcatgg
     6421 cgcccaggat cgacaggttg atcttgccgc cgcggatcat ggcaaagctg tcgtggctgc
     6481 cgaaaatcga cgagccggga atggtggtga cggtctgctt gcccgcattg atcaagtcgg
     6541 cgtccacttc ctcttcggtc ggaaacgggc cgatgcccag catgccgttc tccgactgca
     6601 gccagacttc cttgtcgccg acgtaattgg cgaccagggt cgggatgccg atgcccaggt
     6661 tcacgtagaa accatcctgc agttcctgag cggcgcgcgc cgccatctgt tcttgggtcc
     6721 aacgcatgtc aatctctcct taatctgtta cccgtcatgc cggactcggt ccggcatcca
     6781 ttccggtggg cacgccaccc ggaatgacaa aagggggtta cttggactca ctcaacgtac
     6841 gcttctcgat ccgcttctcg ggatggggat tcacgacgat gcggtgcacg tagatgcccg
     6901 gcagatggat gtcgtcgggc agcagctcac cggtttccac caggtgctcg acctcgacga
     6961 tgcagatctt gccggccatc gccgcggccg ggttgaagtt gcgcgccgtc aggcgaaacc
     7021 gcaggttgcc cgacttgtcg gccacgtggg ctttcaccag tgccacctcg ggtaccaggg
     7081 cccgttccat cacgtaggtt tcgccatcga actcgcgcag ctccttgccg tcggccacga
     7141 tggtgcccac gccggtcttg gtgaaaaacg cgggaatgcc ggcacctccc gcgcgcagct
     7201 tctcggccag cgtgccctgg ggcgtgaact ccagctccag ctcgcctgcc aggtactggc
     7261 gctcgaattc cttgttctct ccgacgtagc tggagatcat cttcctgatc tgccgggatt
     7321 cgagcagctt gcccagcccg aagccgtcga cgcccgcgtt gttcgaaatg acggtgagat
     7381 ccttgacgcc gctgtcgcgc agcgcctcga tcagcgtctc gggaatgccg cacaggccga
     7441 aaccgccgac agccagcagc tggccgtccc tgaccacacc ctggagggcg gcggccgcgg
     7501 agggaaagag cttgttcact aaaagtctcc tgctgagaaa acgggtgtgg aattgcgctg
     7561 gaatgccgtt acgatactac ctaatgagtt acgtagttct ccgtaaaggt tagcccgatg
     7621 gacgtcgact accgccttgc cttcaatctg gcgccggtgg gcctgtgcct gtcgcgcaac
     7681 cgctcgattg tggactgcaa tcagcagctg tgcgacatgt tcggcgcgtc gcgcgagctg
     7741 ctgatcggac agtccttcca ggtgctttac cccagcgccg atgagtatga aaggctgggc
     7801 gcgcgcatgg cgcccatcct caatgccaag gggcactacg ccgacgaccg gatcatgaag
     7861 cgcgccaacg gcgaggtgtt ctggtgccac gtgacgggac gcgcgctcaa ccgcgaagcg
     7921 cctcacgagg ccggcatctg ggcattcgag gacctcagtt cgcaacggcc ggtgaaggcc
     7981 gagctgacgg cgcgcgagcg cgaagtggcg gcccgcctgc tcgacggcat gacgtccaag
     8041 gaaatcggca aggcactggg aatcagccat cgcacggtgg aaatctaccg cgcccgcctg
     8101 atgcgcaagt acaaggccag caccacggcc gacctggtgc acaagttgat ggtgggcgag
     8161 tgagctattt tccgtcgatg acttccgcgg cgatgcggaa ggcatccacg gcggcaggca
     8221 cgccgcaata aatggtggcg tgcaaaagca attcctgcag ttccttgacg gtggcaccgt
     8281 tgttgagtgc cgcccgcaca tgggccttga gttcgtgctg cttgcccagc gccgtcagca
     8341 tggccacggt gaccaggctg
//
LOCUS       DASWIY010000477         7429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_329141, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000477 DASWIY010000000
VERSION     DASWIY010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329141"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VGA59_17130"
     CDS             complement(<1..281)
                     /locus_tag="VGA59_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HEX9721443.1"
                     /translation="MTKRRIPHHVAIVMDGNGRWATQRYLPRVAGHKKGMDALRACVR
                     HCGERGVRVLTVFAFSSENWRRPPEEVSGLLELLAMALGREVPQLKAEGV"
     gene            complement(298..870)
                     /gene="frr"
                     /locus_tag="VGA59_17135"
     CDS             complement(298..870)
                     /gene="frr"
                     /locus_tag="VGA59_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX9721444.1"
                     /translation="MKDTTTTAEIKKAMEGKMDQSIAAFRSNLTKIRTGRANPALLDT
                     IHVDYYGSILPLSQVANVALIDARTISVQPWEKNMSAKIEKAIRESDLGLNPASMGDL
                     IRVPMPPMSEERRKEMTKLVRHEGETAKIAIRNLRRDANEHVKKLVKDKHASEDEQKR
                     SEAEIQKITDKHIVDIDALVHGKEQEIMAV"
     gene            complement(884..1606)
                     /gene="pyrH"
                     /locus_tag="VGA59_17140"
     CDS             complement(884..1606)
                     /gene="pyrH"
                     /locus_tag="VGA59_17140"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX9721445.1"
                     /translation="MPEARPAHKRILLKLSGEALMGDDAFGINRGTIVRMVEEVAEVV
                     HMGVEVAIVIGGGNIFRGVAGGSVGMDRATADYMGMLATVMNALALADAMNKQGLVPR
                     VMSAIAIEQVVEPYVRPKALQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEIGAEVI
                     LKATKVDGVYSADPKKDPAALRYTKLSFDDAMAQNLGIMDATAFALCRDQKLPIKVFS
                     ILKHGALRRVVMGEDEGTLVYA"
     gene            complement(1726..2649)
                     /gene="tsf"
                     /locus_tag="VGA59_17145"
     CDS             complement(1726..2649)
                     /gene="tsf"
                     /locus_tag="VGA59_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX9721446.1"
                     /translation="MAAITASMVAELRGKTDAPMMECKRALTEAQGNMDKAEEILRVK
                     LGNKAGKAAARITAEGVVTASIAGTTGAMLETNCETDFVTKNDGFLAMAKAAAELVAR
                     NNPADLAALAALPYGQDGFGPTLEDVRKGLVGKIGENMSFRRFKRFAGSDKLAVYLHG
                     TRIGVVVAYEGDDVAAKDVAMHVAAMKPVALAAGDVPPELIEKERSIAMAKAEEDRKV
                     AEAAGKAVQSPEIVAKRVEGGVQKFLKEVSLFNQPFVKNDKQTVEQMLKAANTTIKGF
                     TLYVVGEGIEKRADDFAAEVAAQVAAAKGGA"
     gene            complement(2746..3498)
                     /gene="rpsB"
                     /locus_tag="VGA59_17150"
     CDS             complement(2746..3498)
                     /gene="rpsB"
                     /locus_tag="VGA59_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX9721447.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRFWNPKMAPFIFGHRNKIHIINLEK
                     SLPLFQEATKFVRQLAANRGTILMVGTKRQARETVATEARRAGVPFVDQRWLGGMLTN
                     FKTVKTSIKRLKDMKAQQEAGLEAMSKKEQLMFAREMEKLEKDIGGIQDMNSLPDAIF
                     VIDVGFHKIAVSEAKKLGIPLVGVVDTNHSPEGIDYVIPGNDDSSKAVTLYARGIADA
                     ILEGRSSAVTDVVKAVSEGGDEFVEVQEGARA"
     gene            3681..5048
                     /locus_tag="VGA59_17155"
     CDS             3681..5048
                     /locus_tag="VGA59_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9721448.1"
                     /translation="MRSDLIATRESLPSRSGGAPAEMEVSVSIARSPACERAFLETSY
                     DGARAAASSPAAAAQPLAGLAVSVKDLFDVAGQVTAAGSRVLADAPPAAHDSPAVARL
                     RAAGAALIARTNMTEFAFSGVGTNPHFGTPANPADPAVPRIPGGSSSGAAVSVASGAA
                     FIALGSDTGGSIRIPAALCGIVGFKNTARLTPREGALPLSTTLDTVCAMTRSVRDAIL
                     AHEILAARQVALSRLPLAAYRLAVPRTGMLEGLDTTVARAFERALAGLRAEGARIEEI
                     SLAEIRDLGAIQATGGFSAAESYAWHRSLLARRADGYDPRVRARIERGAAMKAYEYIE
                     LIGARADWIARIEAALRDFDAVLSPTVPIVAPPIAQVAPALGVDTVQDAARDEEFFRI
                     NALLLRNPSVVNMLDGCAITIPCHVAGELPVGLMIWHSAMHDDMVLNIALQAQAALAG
                     TSGNS"
     gene            5077..6357
                     /locus_tag="VGA59_17160"
     CDS             5077..6357
                     /locus_tag="VGA59_17160"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019478 - D-amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEX9721449.1"
                     /translation="MRVAVIGAGIIGVTTAFELAVDGQKVTVFERRASVAEEASFANA
                     GVVAPGYVTPWAAPGMAAKVASYLMRSHAPVRVSLPLTREEIAWMWKWYRSCEIGTYT
                     ANRACMQRLAFYSRTRLNEITEHFQLDYDRSVGYMVLLRGEKDARLVQPGLQVLRDAG
                     VTFKEVSAEETRAIEPAISPDMEFVGAIHLPNDEVGNCRQFAMLLKNEAQTLGVNFEF
                     NSAVGPLDPAGPTSLPVWGLDSQAPVRRAFDAVIVCGGVDSIELLRPLGLKIPLAPVY
                     GYSISAPIRESMNAPRSALMDERYKVAIARLGNRVRVAGSAEIGGSLAKKNPAAIQTL
                     YKVLHDWFPGAARIASTGAAVQEWKGARPMLPDGPPLIGATAIPGVWLNLGHGSSGWA
                     LSCGSARVIADLIAGHQPEVDIERLGVQRLALLP"
     gene            6390..>7429
                     /locus_tag="VGA59_17165"
     CDS             6390..>7429
                     /locus_tag="VGA59_17165"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX9721450.1"
                     /translation="MQRVTPNRPWPLFDVAASRALEQAAASRLPPHTLMQRAGLAVAR
                     LSLALAPHARTIWVACGPGNNGGDGFEAAMHLRQWGKNPVVTWLGDEARAPADAQASL
                     QRARQAGVAFAGEAPAQWDLAIDALLGLGSSRPMESAMADRAAALNAAAAPVLAVDLP
                     SGLDANTGAGHGVTATHTLSLLSLKPGLFTAGGRDASGQVWFDDLDVGEPPQEPSAWL
                     TGPAAASMRRHASHKGSYGDVAVIGGAAGMTGAALLAATAALHAGAGRVFAALLDGGT
                     LSVDASQPELMFRSWDSLGLPGMAVACGCGGGDAIRAVLPKVLSTAGALVLDADALNA
                     IAADTQLQTLLQA"
BASE COUNT         1098 a         2516 c         2463 g         1352 t
ORIGIN      
        1 accccctcgg ccttgagctg cggcacctcc cgtcccagcg ccatcgccag cagttccagc
       61 aggccggaga cctcttcggg cgggcggcgc cagttctccg acgagaacgc gaacaccgtg
      121 agcacccgca cgccgcgttc accgcaatgc cgcacgcagg cgcgcagcgc gtccatgcct
      181 ttcttgtggc ccgccacgcg cggcaggtag cgctgggtcg cccaacggcc gttgccgtcc
      241 atgacgatgg caacgtggtg cggaatgcgc cttttcgtca tcgtgccggg atcacggcta
      301 gaccgccatg atctcctgct ccttgccgtg caccagggcg tcgatgtcga caatgtgctt
      361 gtcggtgatc ttctggattt cggcttcgga gcgtttctgc tcatcctccg acgcgtgctt
      421 gtccttcacc agcttcttca cgtgttcgtt ggcatcgcgg cgcaggttgc ggatcgcgat
      481 cttggcggtc tcaccctcgt ggcggaccag cttggtcatc tccttgcgcc tctcctcgct
      541 catcgggggc atcggcacgc ggatcaagtc gcccatcgag gccgggttca ggcccaggtc
      601 gctttcgcgg atggctttct cgatcttggc gctcatgttc ttttcccagg gctggacgct
      661 gatggtgcgc gcatcgatga gcgccacgtt ggcgacctgg ctgagaggca ggatcgatcc
      721 gtagtaatcc acatggatcg tgtccagcag ggcggggttg gcacggccgg tacggatctt
      781 ggtcaggttg ctgcggaagg cggcgatgga ctggtccatc ttgccttcca tggccttctt
      841 gatttcggcg gtcgttgtgg tgtccttcat gtccttgtcc tcctcaagcg tagacgagtg
      901 ttccttcgtc ctcccccatc acgacgcgcc gcagcgcacc gtgcttcaag atggagaaga
      961 ccttgatggg cagtttctgg tcgcggcaga gcgcgaaagc ggtcgcatcc atgatgccga
     1021 gattttgcgc catggcatca tcgaacgaga gtttggtgta acgcagcgcg gcggggtctt
     1081 tcttcgggtc ggccgaatag acgccatcca ccttggtggc cttgaggatc acttcggccc
     1141 cgatttcggc gccgcgcagg gcagccgccg tgtcggtggt gaagaagggg ttgccggtac
     1201 cggcggcgaa gatgacgacc ttgccctcct ccaggtattg gagggccttg ggccgcacgt
     1261 acggctcgac cacctgctcg atcgcgatgg cggacatcac ccgcggaacc aggccctgct
     1321 tgttcatggc gtcggccaag gccagggcgt tcatcacggt ggccagcatg cccatgtaat
     1381 ccgcggtggc ccggtccatg cccaccgaac cgccggccac gccgcggaag atgttgccgc
     1441 cgccgatcac gatggccacc tcgaccccca tgtgcacgac ctcggcgacc tcctcgacca
     1501 tgcgaacgat ggtgccgcga ttgatgccga aggcgtcgtc ccccatcagg gcctcgcccg
     1561 acagcttcag caagatgcgc ttgtgggctg gacgggcttc gggcatgaaa ggactccctc
     1621 tgttggaatg acggatgacg gatgacggat gaccgcgggc gctattttgt catcgatacg
     1681 cggcgaggtc atgcgcgaag cgcgtcattt gtcgtgatga cggcctcagg cgccgccctt
     1741 ggcggcggcc acctgggccg ccacttcggc cgcgaagtcg tcggccctct tttcgatgcc
     1801 ctcgcccacc acgtagaggg tgaatccctt gatcgtggta ttggcggcct tgagcatctg
     1861 ctcgacggtc tgcttgtcgt tcttgacgaa gggctggttg aacagcgaca cctccttgag
     1921 gaatttctgc acaccgcctt caacgcgttt cgccacgatt tcgggcgact gcaccgcctt
     1981 gcccgcggct tcggcgacct tgcggtcttc ttcggccttg gccatggcga tggagcgttc
     2041 cttctcgatc aactcgggcg gcacgtcgcc ggcggccagc gcgaccggtt tcatcgcggc
     2101 cacgtgcatc gccacgtcct tggcggccac gtcgtcgccc tcgtacgcca cgaccacgcc
     2161 gatgcgggtg ccatgcaggt agacggccag cttgtccgag cccgcgaagc gcttgaagcg
     2221 ccggaagctc atgttctccc cgatcttgcc gaccaggccc ttgcgcacgt cttccagcgt
     2281 ggggccgaag ccgtcctggc cgtacggcag cgccgccagg gcggccaggt cggcggggtt
     2341 gttcctcgcg accagctcgg ccgcggcctt ggccatcgcc aggaagccgt cgttcttggt
     2401 gacgaagtcg gtctcgcaat tggtttccag catcgcgccg gtggtgccgg cgatggatgc
     2461 ggtcacgacc ccttcggcgg tgatgcgcgc ggcggccttt ccggccttgt tgcccagctt
     2521 gacgcgcagg atttcctcgg ccttgtccat gttgccctgc gcttccgtca gggccctctt
     2581 gcattccatc atcggcgcgt cggtcttgcc acgcagctcg gcgaccatgc ttgcggtgat
     2641 tgcagccatt cttgtttacc tcgtaatctt tcagctaaaa aacggtatgc gcttgtaaaa
     2701 agaggggctc gaaagccccc cttccctcgg cttgcgaagg ggctctcagg cgcgggcgcc
     2761 ctcttgcact tccacgaatt catccccacc ctcggacacg gccttgacca cgtcggtgac
     2821 ggcgctggag cggccttcca ggatcgcgtc ggcgattccg cgggcgtaca gcgtcacggc
     2881 cttggacgag tcgtcgttgc ccggaatgac atagtcgatg ccttcgggcg agtggttggt
     2941 gtcaaccacc cccaccagcg gaatgcccag cttcttggct tccgacacgg cgatcttgtg
     3001 gaaacccacg tcgatcacga agatcgcatc gggcaggctg ttcatgtcct ggatgccgcc
     3061 gatgtccttc tcgagctttt ccatctcgcg cgcgaacatc agctgctctt tcttgctcat
     3121 ggcctcgagg ccggcttcct gctgggcctt catgtccttc aggcgcttga tcgaggtctt
     3181 gacggtcttg aagttggtca gcatgccgcc cagccagcgc tggtcgacga aaggcacgcc
     3241 ggcgcggcgc gcttctgtgg cgacggtttc ccgggcctgg cgtttggtgc cgaccatcag
     3301 gatggtgccg cggttggccg cgagttgcct gacgaacttg gtggcttcct ggaacagcgg
     3361 gagcgacttt tccaggttga tgatgtggat cttgttgcga tggccgaaga tgaacggggc
     3421 catcttgggg ttccagaagc gggtctggtg cccgaaatgg acaccggctt ccagcatttg
     3481 acgcatggtg acggacatgt ggattcctga aggttgggtc taaaatccag cccgaaattg
     3541 ctgccgcctc gacaaaggcg gcgacaacac cttgttgggt tggattcgcg tatcgtttcg
     3601 ctggtctgcg gtgagcgcac ccttgcgtcc accgcccagc aaaacccaaa aattatagca
     3661 tagccccgcc ctcgaagccc atgcgttccg acctcatcgc cactcgcgaa agcctccctt
     3721 cacgctcggg cggcgccccg gccgagatgg aggtttcggt ctccatcgcc cggtcgcccg
     3781 cctgcgagcg ggctttcctc gagacctcgt acgacggcgc ccgcgcggcc gcgtcgagcc
     3841 cggcggcggc ggcccaaccg ctggccgggt tggcagtgtc ggtcaaagat ctgttcgatg
     3901 tggccgggca ggtcacagcc gccggttcgc gcgtgctggc cgatgcgccc cccgcggcgc
     3961 acgacagccc ggccgtggcc cggctgaggg cggcgggcgc ggccctcata gcgcgcacga
     4021 acatgacgga gttcgcgttc tcgggcgtgg ggacgaaccc gcatttcggc accccggcca
     4081 atcccgcgga tccggccgtg ccgcgcatcc cgggcggctc ctcctcgggc gcggcggtat
     4141 cggtcgccag cggtgcggcc ttcatcgcac tgggctccga caccggcggc tcgatccgca
     4201 tcccggccgc cttgtgcggc atcgtcggat tcaagaacac ggcgcgcctg acgccgcgcg
     4261 agggagcgct cccgctgtcg acgacgctcg acacggtctg cgccatgacc cgctcggtgc
     4321 gcgatgcgat cctcgcgcac gaaatccttg ccgcgcgcca agtggccctt agccggctgc
     4381 cgctcgcggc ttaccgcctc gccgtgcctc gcaccggcat gctcgagggc ctggacacga
     4441 ccgtcgctcg cgcgttcgag cgcgccctgg ccggcttgcg ggccgagggc gcccgcatcg
     4501 aggagatttc cctggccgag attcgcgacc tcggcgccat ccaggctacc gggggcttct
     4561 ccgcggccga aagctatgcc tggcacagga gcctgctcgc gcgccgggcc gacggttatg
     4621 acccgcgcgt gcgcgcgcgc atcgagcgcg gggcggcaat gaaagcatac gaatacatcg
     4681 aacttatcgg cgcacgggcc gattggatcg cccgcatcga ggccgcgctg cgcgatttcg
     4741 acgcggtgct gtcgcccacc gtacccatcg tcgccccgcc gattgcccag gttgcgccgg
     4801 cgctcggcgt ggataccgtg caggacgccg cgcgcgacga ggaattcttc cggatcaacg
     4861 cgctgctgct gcgcaacccc agcgtggtca acatgttgga cggttgcgcc atcaccattc
     4921 cctgccacgt agccggcgag ctgccggtgg gcctcatgat ctggcacagt gcgatgcacg
     4981 acgacatggt attgaacatc gcgctgcaag cgcaggccgc actggccgga acatcaggga
     5041 actcatgaac gttgcgattc ccgccggaag gcggcgatga gggtcgccgt cataggcgcc
     5101 ggcatcatcg gcgtcaccac ggccttcgaa ctcgcggtcg acggccaaaa ggtcaccgtt
     5161 ttcgagcggc gggcatcggt ggccgaggaa gccagctttg ccaatgccgg cgtggtcgcg
     5221 ccgggctatg tcacgccatg ggccgcgccg ggcatggcgg ccaaggtcgc cagttacctg
     5281 atgcgcagtc acgcgcccgt gcgcgtctcg ctgccgctga cgcgcgagga aatcgcgtgg
     5341 atgtggaagt ggtaccggtc gtgcgagatc ggaacctata cggccaaccg ggcctgcatg
     5401 cagcgcctgg ccttctacag ccgcacgcgg ctgaacgaga tcacggaaca tttccagctc
     5461 gattacgatc gcagcgtggg ctacatggtg ctgcttcgcg gcgagaagga cgcgaggctg
     5521 gtgcagcccg gcctgcaggt gctgcgcgac gccggcgtca ctttcaagga agtgagcgcc
     5581 gaggaaaccc gcgcgatcga gcccgccatc agccccgaca tggaattcgt cggcgccatc
     5641 cacctgccga acgacgaagt gggcaactgc cggcaattcg cgatgctgct caagaacgaa
     5701 gcccagacgc tgggcgtgaa cttcgagttc aacagcgccg tgggcccgct ggatcccgcc
     5761 ggccccacat cgctgccggt ctggggcctg gactcgcaag cgccggtcag gcgtgccttc
     5821 gacgccgtga tcgtctgcgg cggcgtggac tcgatcgaac tgctgcggcc gctcggcctg
     5881 aagattcccc tggcgcccgt gtacggctac tcgatcagcg ccccgatccg cgaatcgatg
     5941 aatgcgcccc gtagcgcgct gatggacgaa cgctacaagg tggcgatcgc gcggctggga
     6001 aaccgcgtgc gggtggcggg cagcgccgag atcggcggtt cgctcgcgaa aaagaatccg
     6061 gccgcgatcc agacgctgta caaggtgctg cacgactggt tcccgggcgc cgcgcgcatc
     6121 gccagcaccg gcgccgcggt gcaggaatgg aagggcgccc gccccatgct gccggatggc
     6181 ccgccgctga tcggcgccac cgcgattcct ggggtgtggc tcaacctggg ccacggttcc
     6241 agcggttggg ccctgtcgtg cggcagtgcc cgcgtgattg ccgacctcat cgccggccac
     6301 cagcccgagg tggacatcga gaggctgggt gtccagcggc tggcgctcct gccctaggac
     6361 gcatggcccg ggggtgacag aatcccggca tgcaacgagt cacccccaac cgcccctggc
     6421 cgctcttcga cgtcgcagcc tcgcgcgccc tcgagcaggc cgccgcttcg cgcctgccgc
     6481 cgcacaccct gatgcagcgc gccgggctgg cggtggcgcg gctgtcgctg gccctggcgc
     6541 cccatgcccg gacgatctgg gtcgcgtgcg gccccggcaa caatggcggc gacggtttcg
     6601 aggccgcgat gcacctgcgc caatggggca agaaccccgt ggtgacctgg ctgggcgatg
     6661 aagcacgggc gccggccgac gcgcaggcct ccttgcagcg ggcccgtcaa gcgggcgttg
     6721 cgttcgccgg cgaggcgccg gcgcaatggg acctggcgat cgacgccctg ttgggactgg
     6781 gcagctcgcg gccgatggaa agcgccatgg cggaccgggc cgcggctctg aacgccgcgg
     6841 ctgcgccggt gctcgcggtg gacctgccct ccggactcga cgcgaacacc ggtgccggcc
     6901 atggcgtgac ggcgacccac accttgagcc tgctgtcatt gaagcccggc ctgtttacgg
     6961 ccggcggacg cgacgcatcg ggtcaggtct ggttcgacga cctggatgtg ggcgaaccgc
     7021 cgcaggagcc gagcgcctgg ctcacggggc ctgcggccgc gtccatgcgc cgtcatgcgt
     7081 cgcacaaagg cagctatggc gacgtcgccg tgatcggcgg cgctgccggc atgacgggcg
     7141 ccgcgttgct ggccgccacg gcggcgctgc atgcgggcgc gggccgcgtg ttcgcagctt
     7201 tgctggacgg cggcacgctc agcgtcgatg cttcacagcc cgaactcatg ttcaggtcct
     7261 gggattcgct gggcctgccc ggcatggccg tggcctgcgg ctgcggcggc ggcgacgcga
     7321 tccgcgccgt gctgcccaag gtactctcca cggccggcgc cctggtgctc gacgccgatg
     7381 cgctcaacgc catcgcggcc gacacgcagc tgcagaccct gttgcaagc
//
LOCUS       DASWIY010000478         8921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1452684, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000478 DASWIY010000000
VERSION     DASWIY010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8921
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452684"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VGA59_17170"
     CDS             complement(<1..370)
                     /locus_tag="VGA59_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721451.1"
                     /translation="MSSITSTERYARVIEVSKRVRWEIERDVIRGRRFDYTKTFLPSG
                     LSLVNELAFLSPADKRLLSQVQGRTYAYVFALVERFISAKVLDVSREHWLGDQVALEA
                     LVRMSDEEIKHQEMFRRLEAQ"
     gene            567..1307
                     /locus_tag="VGA59_17175"
     CDS             567..1307
                     /locus_tag="VGA59_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9721452.1"
                     /translation="MNARTRLLVVDDDASVRSMLREYLEGHGYAVAEAGSGAQMREQI
                     EHELPDAVLLDIRLPGEDGLVLARYLREHYEVGIIMVTASGDVVDRVVGLELGADDYI
                     AKPFDLRELLARLKSVLRRMQAKPSAESNPQEAADLQKQPQRRRFGRCEIDLESRRMF
                     EVGGGEVTITAMEYDLLAAFLDNPNRVLTRDQLLLRTRNREWEPFDRSIDIRIGRLRR
                     KVEPDPSGEPRCIRTVRNAGYMFIPNAS"
     gene            1433..2491
                     /locus_tag="VGA59_17180"
     CDS             1433..2491
                     /locus_tag="VGA59_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HEX9721453.1"
                     /translation="MRLGSPRPPLAAEPLDGRPTERQLQPKEAPAVSPSETKSVLTVS
                     LLAAFADGGKHDRERAEIKRIAEGLSQADAVHLPSLYQDVLMKRVSLASAAQGLASLE
                     AKQLAYEMAVCVCDADGVQSVPEQEFLAQLHSVLGLDAGAAGQFTRDAEAIAAAPLTN
                     ANVPSDIGVARTAGSGPADPALDAQLDERILNAAITNGALELLPETLSTMAIIPLQMK
                     LVFGIGKAYGYELDRGHIKDFLATVGVGLTSQYLEQAGRKLLGGLLGSVGGGLLRGLG
                     KQAVSSGMSFAATYALGQVAKRYYAGGRTLSTEMLRDTYAAVIQDAQGLQGRYLPAIQ
                     EKARTLDTGKVLAMVRGA"
     gene            complement(2523..5003)
                     /locus_tag="VGA59_17185"
     CDS             complement(2523..5003)
                     /locus_tag="VGA59_17185"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX9721454.1"
                     /translation="MNQDTFTGNETPTNTVEALRHSIMERLVYSVGKDVETATQRDWL
                     FAVFHAVRDRIVKRWRATLQQSRQQNAKRVYYLSMEYLTGRALVNALLATDLYDVVKQ
                     ACTQLGADFDALIDVEPDPALGNGGLGRLAACFMDSIATIGLPGTGYGLRYEYGMFSQ
                     RILNGQQIEEPDYWLVNGNPWEFMRPELCYTVRFGGRLTSDEDDVVHWVDTEEVIATA
                     YDNGVPGYSLASVATLRLWSARPSTALDLEAFNRGDYMLAVEDKNRSENVTRVLYPED
                     STEHGKELRLRQEYFFVSASMQDILRRYLRNHGNFDALPDKVAIHLNDTHPALAIPEL
                     MRLLIDEHRMGWDDAWSLCTRIFSYTNHTLMAEALETWPVDLMGRVLPRHLRIIFDIN
                     GRLLAGVHAKFPDDPGLLGRVSLVDEHGGRRVRMAYLSVVASHKVNGVSRLHSELIVQ
                     TIFADFARIFPERFCNKTNGITPRRWLALANPPLSALIDTRIGPDWRLHLDQIGQLRP
                     LAEDAEFKSAFRLAKQQNKQRLVKYITDQLHGPKLDPQGLFDVQVKRMHEYKRQLLNV
                     LHVITRYHRILDQPQANWVPRTVVFAGKAASAYYMAKLIIRLINDVARVVNADRVVGD
                     RLKVVFLPDYRVSLAEIVIPAADLSEQISTAGTEASGTGNMKLALNGALTIGTWDGAN
                     IEIAEQVGLDNIFIFGNRTEQVEALRAGGYHPRSYYDNNFDLKYAVDRIADGAFSRDE
                     PGRYADVVRNLLESDYYLLLADYADYVATQDKVDECYREPLDWTRRSILNVAGMGRFS
                     SDRTIQEYALEIWGVAPLAFSSSIRTQP"
     gene            complement(5041..6534)
                     /gene="glgA"
                     /locus_tag="VGA59_17190"
     CDS             complement(5041..6534)
                     /gene="glgA"
                     /locus_tag="VGA59_17190"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX9721455.1"
                     /translation="MKVLYVCTELFPLLKTGGLGDVGAALPPALREAGCDVRVLLPGF
                     PAIRAGVSRTRPPQASPLALPDGEGPRLANLDLAQPPALQPATLPGSGLVAYVLDVRA
                     LFDRPGDPYDDAHGDNGIRFALLGWAAACLGHGLDPNWQPDVVHCHDWHTGLAPLYLR
                     QLAMPGPPPAATVFTVHNLAYQGLFPRSLFGQLGLPDYLYGIEGIEFWGQVSFMKAGL
                     QSADRITTVSPTYAREILEPDQGCGLDGLLRARSGVLSGILNAVDYELWNPSQDAVLS
                     HPYDWDSLENKSMAKAGLQRKVGLQVRPKAIVFGVVSRLTEQKGLHLVEAVADELVHM
                     GGQLAILGAGDGPLEQAFVRAAARHPGQIAIDVGYNEPLAHAMMAGADAVLVPSRFEP
                     CGLTQLYGLRYGALPLVHRVGGLADTVVDATAQNLRAGRATGFAFDEFSAQGLAVALR
                     RAFELHARPDSWAKVQQTAMRQRFDWAEAASRYISLYRSLKPDADLA"
     gene            complement(6531..7814)
                     /gene="glgC"
                     /locus_tag="VGA59_17195"
     CDS             complement(6531..7814)
                     /gene="glgC"
                     /locus_tag="VGA59_17195"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX9721456.1"
                     /translation="MEIQNPTQLARRTIALVLAGGRGSRLAHLTDLRAKPAVYFGGKF
                     RIVDFTLSNCLNSGVRRIGVLTQYKSHSLLRHLQRGWSFLRNEFNEFIDLLPAQQRMD
                     EEFWYRGTADAVYQNLDILLAHNPKYMLVLAGDHVYKMDYAKLIADHVAMDARCTVAC
                     IEVPLAEATAFGVMHVDLQRRIVAFLEKPASPPPMPGKPDRALASMGVYVFDADYLYE
                     ALKQDSANPASNHDFGKDLIPSMVALGHAMAHPFEMSCVRSTQEATAYWRDVGTVDAY
                     WQANLDLTDPLPELDMYDSDWPIWSYQEQLPPAKFVHDEDGRRGHAIDSLVSGGCIIS
                     GARVRRSVLFSSVHVHSYASVEECVLLPEVDVGERCELRRVVADKGCHIPAGMRIGFD
                     AGEDARLFHRTGQGVVLVTRDMLQALEAQPASGPE"
     gene            complement(7978..>8921)
                     /locus_tag="VGA59_17200"
     CDS             complement(7978..>8921)
                     /locus_tag="VGA59_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEX9721457.1"
                     /translation="QLGAFPAGWQEWNDRLRDTARSYWLGHDCTPGLMARRMTGSSEL
                     FHHHGRSPLAGINMVTAHDGFTLADVTAYRHRRNEANGEHNRDGHSLNYSANAGVEGP
                     SRNPAVLRQRAAWRRALLATLLLAQGTPQLLAGDEIGNSQQGNNNAYCQDNTTGWLDW
                     AQADEALTSLVSALTALRKRYPALRHPRWFKGHPFNEAGHPYVPGGDIAWLRPDGLSM
                     SRQDWEDAGNRSFCYVIEVGEGMHPATERVMVLLHPGPSVLAFVLPEGPWRLALDTAL
                     DEWGERRMLGHRYELAGPAICVLVQPVESSRLAGASA"
BASE COUNT         1497 a         2859 c         3067 g         1498 t
ORIGIN      
        1 tctgcgcctc caggcgccgg aacatctcct ggtgcttgat ctcctcgtcg ctcatgcgca
       61 ccagggcctc cagcgcgacc tggtctccca gccagtgctc ccggctgacg tccagcacct
      121 tggcgctgat gaaccgctcg accagggcga acacgtacgc ataagtgcgg ccctgcacct
      181 ggctgagcag gcgcttgtcg gccggggaca ggaacgccag ctcattgacc agcgacaggc
      241 cgctgggcag gaaggtcttc gtgtaatcga agcggcggcc acggatgacg tcgcgctcga
      301 tttcccagcg cacgcgcttg gaaacctcga tgacgcgggc gtagcgctcg gtggaggtga
      361 tgctgctcat ggtgtgtcct ttcgacggtt cggttgatgg agcacgaagt gtcgaaagtg
      421 ccggtatccg gcgcacgtcc gggggcgccg gccatgtagc gatgttgtat tggcggcggg
      481 catgggcgga acgccttgtg gcggctttgt aaccgctttg tattgggggc tcgtcaatac
      541 gaatacgctg cggcacaatc cgccgcatga acgcacgcac acgcctgctc gtggtcgatg
      601 acgacgcatc ggtgcgctcc atgctgcgcg aatacctcga agggcacggc tacgccgtgg
      661 ccgaagccgg cagcggggcg cagatgcgcg agcagatcga gcatgaactt cccgatgccg
      721 tgctgctcga catccgcctc cccggggagg acgggctggt gctggcgcgc taccttcgcg
      781 agcactacga ggtcggcatc atcatggtca ccgcgtcggg cgacgtggtg gaccgggtgg
      841 tcggcctgga gctgggagcc gacgactaca ttgcgaagcc cttcgacctt cgcgagctgc
      901 tggcgcgcct gaagagcgtg ctgcgcagga tgcaggccaa gccatccgcc gaaagcaatc
      961 cgcaggaagc cgcggacctg caaaaacagc cccagcgcag gcggttcggg cgctgtgaaa
     1021 tcgatctcga gtcccgccgc atgttcgagg tgggcggcgg cgaggtcacc atcacggcga
     1081 tggagtacga cctgctggcg gcgttcctcg acaacccgaa ccgggtgctg acccgcgacc
     1141 agctcctgct gcgaacgcgc aatcgcgaat gggagccgtt cgaccgctcg atcgacatcc
     1201 gcatcggccg cctgcgccgc aaggtcgaac ccgatccgag cggcgagccg cgctgcatca
     1261 ggaccgtgcg caacgccggc tacatgttca tcccgaacgc ctcttaggag cggcagcctt
     1321 ctgccgcccg gactccaccg ccccgctcta tcggccgccg gcgatggcgc gcttgccgcg
     1381 cgcccggatg ttgagggctt cgacggcgag cgagaaggcc atcgccgcgt agatgcgtct
     1441 cggatcgcca aggccccctc ttgccgcgga acctttggac ggcaggccga ccgaacggca
     1501 attgcaacca aaggaggccc cggccgtgag tccatccgaa accaagtccg tcctgaccgt
     1561 gagcctgctg gctgcctttg ccgatggcgg caagcatgat cgcgaacgcg ccgagatcaa
     1621 gcgcattgcc gaaggcctgt cgcaggccga tgcggtgcac ctgccgtcct tgtaccagga
     1681 cgtgctgatg aagcgggtgt cactggccag tgccgcgcag gggctggcca gcctggaggc
     1741 aaaacagctg gcgtacgaga tggcggtatg cgtgtgcgac gccgacggcg tgcagtcggt
     1801 gcccgagcaa gagttcctgg cgcagcttca ttccgtgctg ggcctggacg cgggagcggc
     1861 gggccagttc acgcgcgacg ccgaggcaat cgccgcagcg ccgctgacga acgcaaacgt
     1921 gccaagcgac atcggggtcg cgcgaaccgc cggcagcggc cccgccgatc ccgcgctcga
     1981 cgcgcagctg gacgaaagga tcctgaatgc ggccatcacg aacggtgcgc tggagctgct
     2041 gcccgaaacg ctgtcgacga tggccatcat cccgctgcag atgaagctgg tgttcggcat
     2101 cggcaaggcc tacggttacg agttggaccg cggccacatc aaggatttcc tggcgacggt
     2161 gggcgtaggc ctcacgtccc agtacctgga gcaggccgga cgcaaattgc tgggcgggct
     2221 gttgggcagc gttggcgggg gactgcttcg ggggttggga aagcaggccg tgagttcggg
     2281 catgagcttc gccgccacct atgcgctggg ccaggtggcc aagcggtatt acgcgggcgg
     2341 gcgcacgttg tcgaccgaga tgctcaggga cacgtatgcc gccgtcatcc aggacgcgca
     2401 agggttgcaa ggccgctacc tgccggcgat ccaggagaag gcgcgcacgc tggataccgg
     2461 caaggtgctg gccatggtgc gcggggcgta gcgcgatcag gagcgggcat ccgcagggat
     2521 cttcagggct gggtacggat cgaggagctg aaggccagcg gtgcaacgcc ccagatctcc
     2581 aatgcgtact cctggatggt ccggtcggac gagaacctgc ccatccccgc cacgttcagg
     2641 atgctgcgcc gcgtccagtc caagggttcg cggtagcact cgtcgacctt gtcctgcgtg
     2701 gccacgtaat cggcgtagtc ggccagcagc aggtaatagt ccgactcgag caggttgcgc
     2761 accacgtccg catagcgtcc cggttcatcc cgcgaaaaag cgccgtcggc gatccggtcg
     2821 actgcgtact tgaggtcgaa attgttgtcg tagtagctgc gtggatggta gccgccggcg
     2881 cgcagtgctt ccacctgctc cgtgcggttg ccgaaaatga agatgttgtc cagccccacc
     2941 tgctcggcga tctcgatgtt cgctccgtcc caggtgccga tggtgagcgc accattgaga
     3001 gccagcttca tgtttcccgt ccctgacgcc tcggtgcccg cggtggaaat ctgctccgac
     3061 aggtcggcgg ctgggatgac gatctcggcc agcgagaccc ggtagtccgg caggaacacc
     3121 actttcaggc ggtcgcccac cacgcggtcg gcgttgacga cacgggccac gtcgttgatg
     3181 agccggatga tcagcttggc catgtagtag gccgatgccg ccttgccggc gaagacgacc
     3241 gtgcgcggca cccagttcgc ctggggctgg tcgaggatgc ggtggtagcg cgtgatgaca
     3301 tgcagcacgt tcagcagctg gcgcttgtat tcgtgcatgc gcttgacctg aacgtcgaac
     3361 aggccctgcg ggtcgagttt gggcccgtgc aattgatcgg tgatgtactt gaccagccgt
     3421 tgcttgttct gctgcttggc caggcgaaac gcgctcttga attcggcgtc ttccgccagc
     3481 gggcgcaatt gcccgatctg gtccaggtga agccgccagt ccggtccgat gcgggtgtcg
     3541 atcagcgccg acaagggcgg gttggccagc gccagccacc gccgaggcgt gatgccgttg
     3601 gtcttgttgc aaaagcgctc cggaaagatc cgcgcgaagt cggcaaagat cgtctgcacg
     3661 atcagctcgc tgtgcaggcg cgacacgccg ttgaccttgt ggctggccac gaccgacagg
     3721 taggccatgc gcacgcgccg cccgccgtgc tcgtcgacca gcgagacgcg ccccagcagg
     3781 cccgggtcgt ccgggaactt cgcatgcacg ccggccagga gacggccgtt gatgtcgaag
     3841 atgatgcgca ggtggcgcgg cagcacgcgg cccatcaggt ccacgggcca ggtctccagc
     3901 gcttccgcca tcagcgtgtg gttggtgtag gagaagatcc gggtgcacag cgaccacgca
     3961 tcgtcccagc ccatccgatg ctcgtcgatc agcaggcgca tcagctcggg gatcgccagc
     4021 gcagggtggg tgtcgttcag gtggatagcc accttgtcgg gcagggcgtc gaaattgccg
     4081 tggttgcgca gatagcggcg caggatgtcc tgcatcgacg cgctgacgaa gaaatactcc
     4141 tgccgcaggc gcagttcctt gccgtgctcc gtgctgtcct cggggtacag cacgcgcgtg
     4201 acgttctcgg accggttctt gtcctccacg gcgagcatgt agtcgccgcg gttgaaggcc
     4261 tcgagatcga gagccgtgct gggccgcgcg gaccacagcc gcaacgtggc cacgctggcc
     4321 aggctatagc ccggaacgcc gttgtcgtag gcggtggcga tcacttcctc ggtgtcgacc
     4381 cagtgcacca cgtcgtcctc gtcggaggtg agccggccgc cgaaacgcac ggtgtagcaa
     4441 agctcgggtc gcatgaactc ccacgggttt ccgttgacga gccagtagtc gggctcctcg
     4501 atctgctgcc cgttcaggat ccgctggctg aacatgccgt attcgtagcg caagccatac
     4561 cccgtaccgg gcaggccgat cgtggcgatg gagtccatga agcaggccgc cagccggccc
     4621 aggcctccat tgcccagtgc ggggtcgggc tcgacgtcga tgagtgcatc gaaatcggcg
     4681 cccagctgcg tgcaggcctg cttgaccacg tcgtaaagat cggtggccag caaggcgttc
     4741 accagggcgc ggccggtgag gtactccatg gacaggtaat agacccgctt tgcattctgc
     4801 tgcctggact gctggagcgt cgcgcgccag cgcttcacga tgcggtcgcg cacggcatgg
     4861 aagacggcga acagccagtc gcgctgcgtg gccgtttcca cgtcttttcc gacggaatag
     4921 accagccgct ccatgatcga gtggcgaagg gcttccaccg tattggtggg cgtttcgttg
     4981 ccggtaaaag tgtcttgatt cacggtgttg cagtatttgt cagacgcgat tcgcattttt
     5041 tcatgcgagg tccgcatcgg gcttgagtga acgatacagc gaaatgtagc gactggccgc
     5101 ctctgcccaa tcgaagcgct gccgcatggc ggtttgctgc accttcgccc acgagtcggg
     5161 ccgcgcatgc agttcgaagg cgcgccgcag ggcgaccgcc agcccttgtg cactgaactc
     5221 atcgaaggcg aagccggtgg cgcgcccggc ccgcaggttc tgcgcggtgg cgtcgaccac
     5281 ggtgtcggcc agcccgccga cccggtgaac caggggcagg gcgccatagc gcagtccgta
     5341 gagttgcgtg agcccgcagg gctcgaatcg cgagggcacc aggaccgcgt ccgcccccgc
     5401 catcatggca tgggcgagcg gctcgttgta gccgacgtcg atcgcgatct ggcccggatg
     5461 ccttgcggcc gcgcgtacga aggcctgctc cagcggccca tcgcccgccc ccaggatggc
     5521 cagctggccg cccatgtgca ccagctcgtc ggccacggct tcgaccaggt gcaggccttt
     5581 ctgttccgtg aggcggctca cgacgccgaa cacgatcgcc ttcgggcgta cctgcaggcc
     5641 caccttccgc tgaagcccgg ccttggccat gctcttgttc tccagggagt cccagtcata
     5701 gggatgcgac agcacggcgt cttgcgaagg gttccacagc tcgtagtcca ctgcgttgag
     5761 gatgccggaa agcacccccg accgggcacg cagcaaccca tcgagaccgc agccctggtc
     5821 cggctccaga atctcgcgcg cgtaggtcgg gcttacggtg gtgatgcgat cggcggactg
     5881 caagcctgcc ttcatgaagg acacctggcc ccagaattcg atgccctcga tgccgtacag
     5941 gtagtcgggc agccccagtt gcccgaacag cgatcgcggg aagagcccct ggtaggccag
     6001 gttgtggacc gtgaacacgg tcgctgccgg tggcgggccg ggcatcgcaa gctgccgcag
     6061 gtacagcggg gccaggccgg tgtgccagtc atggcaatgc acgacatcgg gctgccagtt
     6121 cgggtccagg ccatgcccca ggcaggccgc cgcccatccc agcaaggcaa agcggatccc
     6181 gttgtcgccg tgcgcgtcgt cgtaggggtc gccgggccgg tcgaacaagg ctcgaacgtc
     6241 gagcacataa gccacgaggc cgctgcccgg caaggtggcg ggttgcagcg ccggtggttg
     6301 ggccaggtcc aggttggcaa ggcgggggcc ctccccgtcg ggcagtgcca gcggcgaggc
     6361 ctgcggcggc ctcgttcggc tcacgccggc ccggatggcg gggaagccgg gcagcaggac
     6421 gcgcacgtcg caaccggcct cgcgcagcgc cggcggcagg gctgcgccga catcgcccag
     6481 accgcccgtc ttgagcagcg gaaacagttc ggtgcagacg tagaggactt tcattccgga
     6541 cccgacgccg gctgggcttc cagcgcctgg agcatgtcac gggtcaccaa caccacgccc
     6601 tgcccggtgc gatggaagag ccgggcatct tcgccggcat cgaagccgat gcgcatgccg
     6661 gctggaatgt ggcagccctt gtcggccacg acgcggcgca actcgcatct ttcgccgacg
     6721 tccacttcgg gcagcaggac gcactcttcc accgaggcgt acgaatggac gtgcacgctg
     6781 gagaacagga ccgagcggcg cacccgggcg ccggaaatga tgcagcctcc cgagaccagg
     6841 gagtcgatcg catggccgcg ccggccgtcc tcgtcatgga cgaacttggc gggcggcaac
     6901 tgctcctggt aggaccagat cggccaatcg gagtcgtaca tgtcgagctc gggcaacggg
     6961 tccgtcaggt ccaggttggc ctgccagtac gcatccaccg tccccacatc gcgccagtag
     7021 gccgtggctt cctgggtgct gcgcacgcag gacatctcga acgggtgcgc catcgcatgt
     7081 cccagcgcca ccatcgacgg aatgaggtcc ttgccgaaat catggttcga cgcgggattg
     7141 gcgctgtcct gcttgagcgc ctcgtacagg tagtcggcgt cgaagacata cacgcccatg
     7201 ctggccagcg cgcgatcggg cttgccgggc atgggcggcg gggaggcggg cttttccaga
     7261 aacgccacga tgcggcgctg gaggtcgacg tgcatcacgc cgaaagcggt cgcttccgcc
     7321 aggggcacct cgatgcaggc caccgtgcag cgcgcgtcca tggccacatg gtccgcgatc
     7381 agcttggcgt aatccatctt gtagacgtgg tcgccggcga gcaccagcat gtacttcggg
     7441 ttgtgggcca gcaggatatc caggttctgg tagaccgcat cggccgtgcc tcggtaccaa
     7501 aactcctcgt ccatccgctg ctgcgccggc agcaggtcga tgaattcatt gaactcgttg
     7561 cgcaggaaag accagccgcg ttgcaggtgg cgcagcaggc tgtgcgactt gtactgcgtg
     7621 agcacgccaa tgcgccgcac gccggagttc aggcagttgg aaagcgtgaa gtcgacaatg
     7681 cggaacttgc ccccgaagta caccgccggt ttcgcgcgca ggtcggtcag gtgcgcaagg
     7741 cgcgagccgc ggccgccggc cagcaccagg gcgatggttc tgcgggcaag ctgggtggga
     7801 ttttgaattt ccatggtgag cgatgggctt gcggccaggg ttggaacagg aaggtttcaa
     7861 tgggattgaa gcaagcggcg gcgccgaggt ctccgcagcc gtgcggtccg ggcagcgacg
     7921 tgggatggag cggcacgccg ctgctgcgac tgtcgaaaat cgagggggcg gcggatgtca
     7981 tgcggatgcc cctgccaggc gcgacgattc gacgggctgg accaggacac agatcgccgg
     8041 gcccgccagc tcgtagcggt ggcccagcat tcgccgctcg ccccattcgt ccagcgcggt
     8101 gtccagagcc aggcgccatg gtccctcggg cagcacgaag gcaagcaccg acgggccggg
     8161 atggagcagt accatgaccc gctcggtggc gggatgcatg ccctcgccca cttcgatcac
     8221 gtagcaaaag ctgcgattgc ccgcgtcttc ccaatcctgc cgcgacatcg aaaggccatc
     8281 gggacgcagc caggcgatgt cgccgcccgg gacataggga tgcccggctt cgttgaacgg
     8341 gtggcctttg aaccagcgtg gatggcgcaa cgcggggtag cgctttctca gcgccgtcag
     8401 ggcggacacc agggaggtca gcgcctcatc ggcctgcgcc cagtcgagcc agccggtcgt
     8461 gttgtcctgg cagtaggcgt tgttgttgcc ttgctggctg ttgccgatct cgtcgccggc
     8521 cagcagctgg ggcgtacctt gggccagcaa cagggtggcc agcagggccc gccgccacgc
     8581 ggcgcgctgg cgcagcaccg ccgggttgcg gctcggcccc tcgactccgg cgttggcgct
     8641 gtagttgagg ctatggccat cccggttgtg ttcaccgttg gcctcgttgc ggcgatgccg
     8701 gtaggcagtg acgtccgcca gggtgaagcc gtcgtgcgcc gtcaccatgt tgatgccggc
     8761 gagcgggctg cgcccatggt ggtggaacag ttcgctggag ccggtcatgc gccgcgccat
     8821 cagccccggc gtgcagtcgt gccccagcca gtaggaacgc gcggtgtcgc gtaagcggtc
     8881 gttccactcc tgccacccgg cggggaaggc gccgagctgg t
//
LOCUS       DASWIY010000479         8524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2216853, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000479 DASWIY010000000
VERSION     DASWIY010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8524
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216853"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VGA59_17205"
     CDS             <1..808
                     /locus_tag="VGA59_17205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX9721458.1"
                     /translation="AHALNESREELRRLSGLLVSFQEDERRRIALDLHDGLGQSISLI
                     KLAIEKSAGLLAAGATCEAGESLRQLIPRVEEALVEVRRVSTDLRPLMLDDLGILPTL
                     SWFFREFETVCGGLVVEKTLNVAEKDVPAPLHITLYRILQEAFHNIVKHAGADRVRVR
                     LDRIDDALQLSIEDNGRGFDPDSVKWVNGQTRGLGLLSMKERASFSGGTYLLDSAPGK
                     GTCIKVTWPAGICPAAVDCCRMSDCSGAQVPCRPMKTTQFGDPKNTVHRV"
     gene            921..1367
                     /gene="gspG"
                     /locus_tag="VGA59_17210"
     CDS             921..1367
                     /gene="gspG"
                     /locus_tag="VGA59_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX9721459.1"
                     /translation="MQKTRYDMGGASALRNGRGFTLLELLVVMVIIGLLAGYVGPRYF
                     AQIGKSEVKAARAQLDALSKALDQYRLDTGHYPTSEEGLATLVERPANEPKWGGPYLV
                     KAVPPDPWGNPYVFIMPGEHGEYDLLSYGKDGKPGGEGEAAEITNW"
     gene            1382..2572
                     /locus_tag="VGA59_17215"
     CDS             1382..2572
                     /locus_tag="VGA59_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9721460.1"
                     /translation="MRYQVKALRGEDELTAFALEAADAGDAASQVRDQGYTVIAVSPK
                     AAWPAWRKAGTGRFPLVLFSQELLALLEAGLSLVESLETLAEKEQQPEIRKTLTQMIA
                     ALYEGHTLSHALQHSTANFPLLYVATVRASEKTGGLPEALSRYVAYQKQMDAVRRQVV
                     SASIYPLLLAGVGALVTLFLMAYVVPRFSHIYAEMGGELPLMSRLLMGWGQLLDEHGA
                     AILTAAVLGLLAATYAATRPATRQWLMRKLWQLPSVGRRLHVFQLARFYRSLGMLLRG
                     GMPVVTSLQMVSDLLESSLRGQLGLAAARIREGHALSSSMEQYGLTTPVASRMLRVGE
                     RTGRMGEMMERIAAFYEEDTARWVERFTKLFEPLLMVFIGLVIGGIVVLMYFPIFELA
                     GSIQ"
     gene            2575..4269
                     /locus_tag="VGA59_17220"
     CDS             2575..4269
                     /locus_tag="VGA59_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX9721461.1"
                     /translation="MAEPIDADTGSGFSPEQVHSARDAARQARRASVQVLEEQSGLDP
                     QRFVRALAATLHYPAAAMEDLHRMAPAFDVLPFGEALERQCLALRDQNDALVLVMGDP
                     FDCDLQAWAEQRVPARFRWCLAHSADVAAYLARHEESLRAMDGLLPSGERDAGARSDA
                     ADLSFKSISEDTSPVVKLVHSTVYDALKAGVSDIHLESTPGGLAIRYRIDGVLTVVGS
                     LAGLELAEQVISRIKIMSELDIAERRIPQDGRFKVSVHGREIDFRVSIMPSIFGEDAV
                     LRILDKQTLSDQIKGLRLDYLGFDERTLARLRRLSSEAYGMLLVTGPTGSGKTTTLYA
                     ALTEINHGQDKIVTIEDPVEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGE
                     IRDSETAQIAVQSALTGHLVFTTVHANNVFDVIGRFIHMGVDPYSFVSAMNGIMAQRL
                     LRVSCPHCAEADQPDAHLLVESGISADQAQHMTFRAGRGCGHCRGTGFKGRRAIGEIL
                     YLNDEIREAIIARQPIRVIREAARRNGTRFLRELALEMVGRGETTLKEINRVTLAV"
     gene            4250..5101
                     /locus_tag="VGA59_17225"
     CDS             4250..5101
                     /locus_tag="VGA59_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721462.1"
                     /translation="MSPWLSKELRVVLCPDQLMLMPVQRTLTLRGVRRSIQEPQVVCL
                     EGASAAQPWRAALLALETALPAAVAKGRTTATVVLSNQLLRYALVPWQAGLADAQEDL
                     SYTRHCFTKVYGKAALQWEVRLSHQAPGKPRLASAVDAELLEGLRSMFARLGIALRSV
                     QPHLMAAFNDARSQLRRRSAWLALLEPGHLCLALLRDGHWSRVRSLRIDSAWRQELPR
                     ILEREAFLVDDAAVPHDVFVGCPEAGELVLPEVDGWQFHALEPAPAVSSASPEPGHVA
                     MAMAMAG"
     gene            5107..5646
                     /locus_tag="VGA59_17230"
     CDS             5107..5646
                     /locus_tag="VGA59_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721463.1"
                     /translation="MRALQLDFQRTYKPAPWLGLGVLVAALVALAAMAGYYQTLNEQI
                     RYWQDKADQVTRLPGHRAAVSPPLTQQAARAQMLEVKQANRVVRQLGQPWNALFNAVE
                     SSGKEVALLSLEPDLQKGMVTIGGEAKDFDALLNYVKELSAREVFASVMLQNHQIQRE
                     IAEKPVRFSLIAHLKGVAP"
     gene            5643..6194
                     /locus_tag="VGA59_17235"
     CDS             5643..6194
                     /locus_tag="VGA59_17235"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HEX9721464.1"
                     /translation="MKLRQSFAIRREHVHLGVPGAIGVGALALCLGLYVSMLQPAEQR
                     RDTARLAATSLQKRMAQAGPDWNGRAPLGEQLAAFYRIFPGEQDATDSVGKIAAVAKR
                     HRLVLYQAEYKAERDKTGKLTRFQMNLPLKGDYRTLRRFLADLHAEMPTVSLEQVQFE
                     RQKVGDPVVDARVRLVIYLGRSS"
     gene            6191..6715
                     /locus_tag="VGA59_17240"
     CDS             6191..6715
                     /locus_tag="VGA59_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721465.1"
                     /translation="MSPRTWRRYALFGALALVLVLSAVSGKGWNSAGEPVASSPVRPR
                     QVSRAPVQQLPTIELEKMQRRDGPPDPDKVAINAFGAMSWRVEPPAPPKAVVKPAPPP
                     PPSAPPMPFTFLGRYEEGGTRTIVLVKGERIYTVSEGDVIDQTYRVERLAGGQLELTY
                     LPLGIKQTISAGGS"
     gene            6715..>8524
                     /locus_tag="VGA59_17245"
     CDS             6715..>8524
                     /locus_tag="VGA59_17245"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX9721466.1"
                     /translation="MNAAIVKRMSGNAVRAVLLAALLAGCAGQQLHREGMTLLAEGRV
                     EEGLNKLAQASSAAPDNVSYRADLVRSRDQMVSRLLAAADSERAAGRPAAAQALYERI
                     LNIHPGNSRAVVGLEMLQMDARHEEALARADALLKKRDAEAARAALAPVLLENPRNGK
                     ALMLQRKINEQLAREMPPEPSLKAKFTKPVTLQFRDANLKMVFEALSRISGINVLLDR
                     DVKADLKTSIFVKDVSVEDTINLIALQNQLEKKVISDNTVFIYPATAAKLKEYQDLKV
                     RSFHLANADPKQMLTMIKTLLKTKDVFIHEKTNSIVMRDTPDAIRLAEKMIADQDIDQ
                     PEVMLEVEVLEVTTSRLSELGIRWPGSVTLSTPAAAATLGQMRDLVSRDLLVTPQSAT
                     LNLALEDGDTNVLARPRIRVRSREKAKIMIGSRVPVITNAVTPVSTGTPVITGSVTYL
                     DVGLKLDVEPDIQLDNEVVIKVSLDVSSILREVLNVQSGSLAYEIGTRNATTVLRLRD
                     GETQVLAGLINDEDRKTASKVPGLGQLPVIGRLFSSHRENGTKTEIVLSITPRIVASA
                     RLPDVTEVEYWTGTEASLRNGPLSMNPMGTVALTSGT"
BASE COUNT         1599 a         2656 c         2823 g         1446 t
ORIGIN      
        1 agcgcacgcc ttgaatgaat cccgggaaga gctgcgacga ctctccggcc ttcttgtatc
       61 gttccaggag gacgagcgca ggcgcattgc actggacttg cacgatgggc tgggtcagtc
      121 catcagcctg atcaagctcg ccatcgagaa atccgccgga ctgctggccg cgggtgcgac
      181 ctgcgaggcc ggcgagtcgc tgcgacagtt gatcccccgg gtggaggagg cactcgtcga
      241 agtgcgccgg gtatccacgg atttgcggcc gttgatgctc gatgacctgg gcatcctgcc
      301 cacgctgtca tggttcttcc gtgaattcga gaccgtgtgc ggtggcctcg tggtcgagaa
      361 gacactcaat gtcgccgaga aagacgtgcc ggcgcccctg catatcaccc tgtatcgcat
      421 tcttcaggaa gccttccaca acattgtcaa gcacgcgggc gccgaccggg ttcgggttcg
      481 cctggaccgt atcgatgatg ccctgcaact gtcgatcgag gacaacggcc gcggattcga
      541 tccggacagc gtcaagtggg tcaacggcca gaccaggggg ctgggactgc tcagcatgaa
      601 ggagcgcgca tcgttttcag gaggaaccta cctcctggac tccgcccccg gcaaaggcac
      661 ttgcatcaag gtgacctggc ccgcaggcat ttgtccggcg gccgtcgatt gctgtcgcat
      721 gtccgattgc agcggagcac aggtcccctg ccggccgatg aagactacgc agtttggcga
      781 cccgaaaaat actgttcacc gtgtatgaac gtataccgtg aaacactcag agccagagtg
      841 cacctgcggc actaaggtag gagcagcgct tgggaaattg aagttttatc agacgagaac
      901 aggcagtgga gacgctggaa atgcagaaaa cacggtatga catgggcggc gcctccgcac
      961 tccgaaatgg gcggggtttt accctgctcg agctgctcgt tgtcatggtg atcatcgggt
     1021 tgctggccgg ctatgtgggg cccaggtatt ttgcccagat cggcaagtcg gaagtcaagg
     1081 cggcacgcgc ccagctcgac gcgctgtcca aggcgctgga ccagtatcgg ctcgacaccg
     1141 gccattaccc caccagtgag gaggggctgg cgacgctggt cgagcgtcct gccaacgagc
     1201 ccaaatgggg cggcccgtac ctggtcaaag ccgttccgcc cgatccatgg ggcaacccct
     1261 acgtgttcat catgccgggc gagcatggag agtacgacct gctgtcgtac ggcaaggacg
     1321 gcaagcccgg cggcgaaggc gaagccgcgg aaattacgaa ctggtagtag gaacgaacac
     1381 catgcgctac caggtcaagg cattgcgcgg cgaagatgag ctgacggctt tcgcgctcga
     1441 agccgcggat gccggcgatg cggccagcca ggtgcgggac cagggataca cggtgatcgc
     1501 ggtcagcccc aaggcggcct ggccggcctg gcgcaaggcg ggcacgggca gatttcccct
     1561 ggtgctgttc agccaggagt tgctggccct gctggaagcg gggctatcgc tcgtggagtc
     1621 cctggaaacc ctggccgaaa aggagcagca gcccgagatc cgtaaaacac tcacgcaaat
     1681 gatcgccgcc ctgtacgaag ggcatacgtt gtcccatgcc ctgcaacatt ccacagccaa
     1741 ctttccgctg ctgtatgtag caaccgtgcg cgccagcgag aaaaccggcg gcttgccgga
     1801 agcattgtcg cgctacgtcg cgtatcagaa gcagatggat gccgtcaggc ggcaggtggt
     1861 cagtgcctcg atctatccac tccttctggc cggtgtcggc gcgctggtga cgctgtttct
     1921 catggcgtac gtggtgccgc gtttcagcca tatctatgcc gaaatgggcg gtgagctgcc
     1981 actgatgtcg aggctgctga tgggctgggg ccaactgctg gatgagcatg gcgcagcgat
     2041 cctcacagcc gccgtgctgg ggctgctggc ggcgacctac gcggccaccc ggccggctac
     2101 caggcaatgg ctgatgcgca aactgtggca gctacccagt gtcggcaggc gtctgcatgt
     2161 ttttcagctt gcccgcttct accgttcgct tgggatgttg ctgcgcggcg gcatgccggt
     2221 ggtgacttcc ctgcagatgg tgtcggacct gctggagtca tccctgcgcg gccagctggg
     2281 gttggccgcg gcccgcatcc gcgaaggcca tgcgctttcc agctcgatgg agcaatacgg
     2341 tcttacgacg cccgtcgcgt cgcgcatgct gcgcgtgggt gagcgcaccg gacggatggg
     2401 cgagatgatg gaacgcatcg cggcgtttta tgaggaagac acggcgcgct gggtcgaacg
     2461 cttcaccaag ctgttcgagc ctttgctgat ggtgttcatc gggctggtca tcggcggcat
     2521 cgtggtgctg atgtatttcc cgatcttcga acttgcgggg agcatccagt gaacatggcg
     2581 gaacccatcg atgccgacac cggttccggg ttttcaccgg agcaggtgca ctcggcgcgc
     2641 gacgcggcac ggcaggcgcg gcgcgccagc gtccaggtgc tggaggaaca gtccgggctc
     2701 gatccgcagc gcttcgtgcg ggcgctggcg gcgacgctgc attatcccgc cgcggcgatg
     2761 gaggatctgc accgcatggc tccagccttc gacgtgctgc cgtttggcga ggccctggaa
     2821 cgccagtgcc tcgccctgcg tgaccagaac gacgcactgg tgctggtcat gggcgaccct
     2881 ttcgactgcg acctgcaggc gtgggccgag cagcgcgtgc ccgcccgttt cagatggtgc
     2941 cttgcgcact ccgccgacgt ggccgcgtac ctggcacgcc acgaggaatc gctgcgtgcc
     3001 atggatggcc tgctgcccag cggcgagcgc gacgccggcg cgcgcagcga tgccgccgac
     3061 ctgtcgttca agagcatcag cgaagacacc agcccggtcg tcaagctggt gcattcgacg
     3121 gtgtacgacg cgctgaaagc gggggtgagc gatatccatc tggagagcac accaggcggc
     3181 ctcgcgatcc gctaccgcat cgacggggta ctgaccgtgg tcgggtcgct ggccgggctg
     3241 gagctggcgg agcaggtgat ctcccgtatc aagatcatgt ccgaactgga cattgccgag
     3301 cgccggattc cgcaggacgg ccgcttcaag gtgtcggtgc atggccgcga aatcgatttc
     3361 cgcgtgtcca tcatgcccag catcttcggc gaggacgcgg tcctgcgcat cctggacaag
     3421 cagacgctgt cggatcagat caaggggctg cgcctcgact atctcggctt cgacgagcgc
     3481 acgctggccc ggctgcgccg tctgtccagc gaggcctacg gcatgctgct ggtgaccggt
     3541 cccaccggct cgggcaagac caccacgctg tatgcggcgc tgaccgagat caatcacggc
     3601 caggacaaga tcgtcaccat cgaagacccg gtggagtacc agttgccggg cgtcctgcaa
     3661 atccccgtca acgagaagaa agggctgacg tttgcgcgcg gcctgcgttc catcctgcgc
     3721 catgatccgg acaagatcat ggtcggcgag atccgggact cagaaaccgc gcagatcgcg
     3781 gtgcaatcgg cgttgacagg ccacctggta ttcaccacgg tgcacgccaa caacgtgttc
     3841 gacgtgatcg gccgcttcat ccatatgggg gtggacccct acagctttgt ttcggcgatg
     3901 aacggcatca tggcccagcg gctgctgcgg gtgagctgcc ctcactgcgc cgaggcggat
     3961 cagcctgacg cccatctgct ggtggaatcc ggcatctccg ccgaccaggc gcaacacatg
     4021 accttccgcg cggggcgagg ctgcggtcat tgccgcggta ccggcttcaa gggacgccgc
     4081 gcgatcggcg agatcctgta cctgaacgac gaaatccgcg aagccatcat tgcccgccag
     4141 ccgatccgtg tgattcgcga ggcggcaaga cgcaacggca cccgtttcct gcgcgaactg
     4201 gcgctggaaa tggtcggccg aggcgaaacg acattgaagg agatcaaccg tgtcaccctg
     4261 gctgtctaag gaattgaggg tggtcttgtg cccggatcag ctgatgctta tgccggtgca
     4321 gcgcacgctc accctgcgcg gcgtgcgccg ctccatccag gagccgcaag tggtatgcct
     4381 ggaaggtgca agcgccgctc agccatggcg cgctgctctg ctggcactgg agaccgcatt
     4441 gcctgccgcc gttgcaaagg gaaggaccac ggccaccgta gtcctgtcca atcaattgct
     4501 gcgctacgcg ctggtgccct ggcaggccgg gctggctgac gcgcaggagg acctctccta
     4561 cacccgccat tgctttacca aagtgtatgg aaaggccgcc ctgcaatggg aagtgcgcct
     4621 gagccaccag gcgcccggaa agcccaggct ggcgagcgct gtggatgccg agttgctcga
     4681 aggcctgaga agcatgtttg cccggctggg catcgcgctg cggtccgtgc agccccatct
     4741 gatggccgcc ttcaacgatg cgaggagcca gctgcggcgg cgcagcgcat ggctcgcgct
     4801 gctcgaaccc ggccacctgt gtctcgcgct gctgcgcgat gggcattggt cgcgggtgcg
     4861 cagcctgcgc atcgacagcg catggcgcca ggaactcccg aggatcctcg agagggaagc
     4921 cttcctggtg gatgacgcgg ccgtacccca tgacgttttc gtggggtgcc cggaagccgg
     4981 tgagctggtg cttccggagg tcgatggctg gcagtttcat gcgctcgagc ccgcgcctgc
     5041 ggtcagttcc gcgtcaccgg aacctgggca tgtggccatg gcgatggcga tggcggggtg
     5101 atcaccatgc gtgccctgca gctcgatttc cagcgcacct acaagcccgc gccgtggctc
     5161 gggctcggcg tgctcgtcgc ggcactggtc gcgctcgccg cgatggccgg ctattaccag
     5221 acgctgaacg agcagatccg ctactggcaa gacaaggccg accaggtcac acggctgccg
     5281 ggtcaccgcg cggctgtctc gccaccgctc acccagcagg ccgcgcgtgc gcagatgctg
     5341 gaggtcaagc aggccaaccg ggtagtgcgc cagcttggcc agccatggaa cgcactgttc
     5401 aacgcggtgg agagttcggg gaaagaggtt gccctgttgt cgctggagcc agacctgcaa
     5461 aaaggcatgg tgacgatcgg cggcgaagca aaggatttcg acgccttgct gaactacgtg
     5521 aaggaactgt cggcgcgtga agtgttcgcc agcgtcatgt tgcagaacca ccagatccag
     5581 cgtgagatcg cggaaaagcc ggtgcggttt tccttgatcg cgcacttgaa gggggtggca
     5641 ccgtgaaact tcgccaatct ttcgccatcc ggcgcgagca cgtgcatttg ggcgtgccag
     5701 gcgccatcgg ggtgggggcg ctcgcactgt gcctgggcct ctatgtttcg atgctgcaac
     5761 ccgcggagca gcgccgcgac accgcgcgcc tggccgcaac ttcgctgcag aagcgcatgg
     5821 cacaggcggg cccggactgg aacggaaggg cgccgctggg cgaacaactc gccgcctttt
     5881 accggatctt tcccggcgag caggacgcga ccgattccgt cggcaagatt gccgccgtcg
     5941 ccaagcgcca ccgtctcgtt ctctaccagg ccgagtacaa ggccgaacgc gacaagaccg
     6001 gcaagctgac ccgcttccag atgaacctgc cgctcaaggg agactaccgg accctgcgca
     6061 ggttcctcgc cgacttgcac gccgaaatgc cgaccgtctc tctggaacag gtgcagttcg
     6121 agcgtcagaa agtgggcgat ccggtggtgg acgccagagt ccggctcgtg atctacctgg
     6181 ggagatcctc atgagcccgc ggacgtggcg acgctacgcc ctctttggtg cgctggccct
     6241 ggtgctggtg ttgtctgccg tttctggaaa ggggtggaat agcgctggcg agccggtggc
     6301 ctcgtccccc gtgcgcccgc ggcaggtgtc gcgagcgccg gtgcaacagc tgcccacgat
     6361 cgagctcgaa aaaatgcaga gacgcgatgg cccccccgat cccgacaagg tggccatcaa
     6421 tgcattcggt gcgatgtcct ggcgtgtgga gccgcccgcc ccgccgaagg cagtcgtcaa
     6481 accggcgccg ccgccgcccc cttcggctcc gccgatgccg ttcaccttcc tgggccgtta
     6541 cgaggagggg ggaacgcgga ccatcgtgct ggtcaaaggc gagcggatct acaccgtttc
     6601 ggagggcgac gtgatcgacc agacctaccg cgtcgagcgt ctggcaggcg ggcagctgga
     6661 gctgacctac ctgccgcttg gcatcaagca aaccatcagt gcggggggga gttgatgaat
     6721 gccgccatcg tcaagcgcat gagcggaaac gcagtacgcg cggtgttgct ggcggccttg
     6781 ctggcgggat gcgccgggca gcaactgcac cgcgaaggca tgacgttgct ggcggagggg
     6841 cgcgtcgagg aaggtttgaa caagctggcg caggccagca gcgctgcgcc ggacaacgtg
     6901 tcctaccgcg cggacctcgt gcgcagccgg gaccagatgg tgagccggct actggcggcg
     6961 gccgacagcg aacgggcggc cggccggccg gcggccgcac aggctttgta cgaacgcatc
     7021 ctgaacattc atccgggcaa cagccgggcg gtggttggcc tcgaaatgct gcagatggat
     7081 gcgcgtcacg aggaggcgct ggcacgcgcg gatgcactgc tcaagaaacg ggacgcggag
     7141 gccgcccggg ccgcactggc acccgtcttg ctggaaaacc ccaggaacgg caaggcgctg
     7201 atgctgcagc ggaagatcaa cgagcagctg gccagggaga tgccacccga accctcgctg
     7261 aaggccaagt tcaccaagcc ggtgaccctg caattccgcg acgccaatct caagatggtg
     7321 ttcgaggccc tgtcgcgcat cagcggcatc aacgtgctgc tggaccggga cgtcaaggcc
     7381 gacctgaaaa cctcgatttt cgtcaaagac gtgtcggtag aagacacgat caacctcatc
     7441 gcgctgcaaa accagctgga aaagaaagtc atcagcgaca acacggtgtt catctatccg
     7501 gccacggctg caaagctcaa ggaataccag gacctgaaag tcaggagctt tcacctggcc
     7561 aatgccgatc cgaagcagat gctcaccatg atcaagaccc tgctgaagac caaggacgtc
     7621 ttcattcacg agaagaccaa ctccatcgtg atgcgcgaca cgcctgacgc catccggctc
     7681 gcggaaaaaa tgatcgccga ccaggacatc gaccagcccg aagtgatgct ggaagtcgag
     7741 gtgctggaag tgaccacctc gcgcctgagc gaactgggga tcagatggcc gggcagcgtc
     7801 acactgagca cgccggccgc ggctgcgacg ctggggcaaa tgcgggacct ggtgagcagg
     7861 gacttgctgg tcactcccca gagcgccacg ctcaacctcg cactggaaga cggcgatacc
     7921 aacgtcctgg ccaggccgcg catccgggtg cgtagccggg agaaggcgaa gatcatgatc
     7981 ggcagccggg tgccggtgat cacgaatgcg gtgacgcccg tttccaccgg cacgccggtc
     8041 atcaccggca gcgtgacgta cctggacgtc ggcctgaagc tggacgtgga gcccgacatc
     8101 cagctggaca acgaggtggt gatcaaggtg tcactggacg tcagttcgat cctcagggaa
     8161 gtgctcaacg tgcaatcagg ctcgcttgcc tatgagatcg gcacccgcaa cgccaccacg
     8221 gtgctgcgcc tgagagatgg tgaaacgcag gttctggcgg gcctgatcaa cgatgaggat
     8281 cgcaagaccg ccagcaaggt gcccggactt ggccagttgc cggtgatcgg ccggctgttc
     8341 tccagccacc gggagaacgg cacgaaaaca gaaatcgtgc tgtccatcac cccgcgcatc
     8401 gtcgccagcg cccgcctgcc cgacgtgacc gaagtggagt attggaccgg caccgaggcc
     8461 agcctgcgca acgggccgct cagcatgaat cccatgggca ccgtggccct caccagcgga
     8521 accg
//
LOCUS       DASWIY010000480         6699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1454721, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000480 DASWIY010000000
VERSION     DASWIY010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454721"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VGA59_17250"
     CDS             complement(<1..589)
                     /locus_tag="VGA59_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX9721467.1"
                     /translation="MQLPINSFKQALREKRPQIGLWVGLADHYSTEICAGAGFDWLLL
                     DGEHAPNDLRTLLQQAQVVAGYPGTHPIARVPVGHGHAGTALIKQYLDLGIQTLLVPM
                     VDTAQQAADLARATRYPPDGIRGMGGARASRWGRFPNYANEANAQVCLLVQAETRMAL
                     DNLEAIAAVDGVDGIFIGPADLSASLGHVADPGHPE"
     gene            complement(639..1598)
                     /locus_tag="VGA59_17255"
     CDS             complement(639..1598)
                     /locus_tag="VGA59_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX9721468.1"
                     /translation="MDKLTLTAAVAQTASVLLDAPATVERALALMGEAARRDVQLLVF
                     PEAFIGGYPKGADFHIYLGARTPQGRAEFKHYFDAAVAVEGPEIAQLAKAAAQHRMYV
                     VVGIIERDGGTLYCTAVYLGPQGQVLGKHRKLMPTALERLVWGFGDGSTLAAVDTPYG
                     KLGAVICWENYMPALRMAMYQQRIALYCAPTADDRDTWASTMQHIAMEGRCFVLSACQ
                     HLRRSQFPGDRMNNRLPEAPETVLMRGGSMIIDPLGKVLAAPVYNEDALLTAELDMSA
                     IAMAQMDFDPVGHYARPDVFSLRVNTAPQRAVSLEDGEGSGQG"
     gene            1715..3091
                     /gene="radA"
                     /locus_tag="VGA59_17260"
     CDS             1715..3091
                     /gene="radA"
                     /locus_tag="VGA59_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX9721469.1"
                     /translation="MAKEKTSYICTECGGASPRWLGKCPHCEAWNTLIETVPESGGAG
                     RNRFASLAKPAEVAALADIEASEIERTPTGIGELDRVLGGGIVEGGVVLIGGDPGIGK
                     STLLLQALDSLQRSAVPTLYVTGEESGAQVALRSRRLGLDNSQVKVLAEIQLEKILAT
                     IAAVQPAVAVIDSIQTVYSDQLTSAPGSVAQVRECAAHLTRAAKSGGTSIVLVGHVTK
                     EGALAGPRVLEHMVDTVLYFEGDTHSSFRLVRAVKNRFGAVNEIGVFAMTEKGLKGVA
                     NPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVDSGGVSPRRLSVGLDRDRLAM
                     LLAVLHRHAGVACMDQDVFVNAVGGVRISEPAADLSVMLAITSSLRGKPLPKGFIAFG
                     EVGLAGEVRPAPRGQERLREAAKLGFSVAVVPRANAPKRGSREIEGLTIHPVERIEEA
                     MDVVRGLG"
     gene            3104..3511
                     /locus_tag="VGA59_17265"
     CDS             3104..3511
                     /locus_tag="VGA59_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX9721470.1"
                     /translation="MNLQQILMPVLGVVLVAAAWHVYGWGGVAIAAGGIVMWGLLHFT
                     RLTHVLSRAAERPVGYVASAVMLNAKLKPGVNLLHVIAMTRALGERLSKEGEQPELYR
                     WTDGGASSVTCEFANGRLVKWTLARPPAQADGT"
     gene            complement(3530..4198)
                     /locus_tag="VGA59_17270"
     CDS             complement(3530..4198)
                     /locus_tag="VGA59_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX9721471.1"
                     /translation="MDDIPGPATLAPTTLIDSDHPAVIEFSQRATGADARQRAVSLYY
                     LVRDGFRYDPYHIDLSVAGLRASRVLEAGYGWCVPKAALLAALSRAAGIPARVGYADV
                     RNHLSTERMRKIAGTDLYIWHGYTEMWLDGAWVKATPAFNLTLCERFGILPLDWDGRS
                     DSLYHPYDRAGRHHMEYVNQRGSFEDVPAARIKADFTAMYPDWVASAAPDADFEKDAE
                     RESR"
     gene            4345..4965
                     /locus_tag="VGA59_17275"
     CDS             4345..4965
                     /locus_tag="VGA59_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HEX9721472.1"
                     /translation="MPCYAIDGVVPVVDPSAFVHPTAVLIGDVIVGPNCYVGPCASLR
                     GDFGRIVLGPGANVQDGCIIHGYPKQDTLVEENGHIGHGAVLHSCVVRRGALVGIRAV
                     VMDDVEIGEHAVVAACAFVPVGRHVAPRTMVAGIPAKVVRTLKGDKEIDWYAEGTRAY
                     HELTGRCLASLRETQPLAAVEPDRLRLPVSKVDALIVARDRGIREA"
     gene            5009..5941
                     /locus_tag="VGA59_17280"
     CDS             5009..5941
                     /locus_tag="VGA59_17280"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX9721473.1"
                     /translation="MAPPSMADRDGKIWMDGELVEWRDAKIHVLTHTLHYGCGVFEGV
                     RAYNTVNGTAVFRLQEHTDRLFNSAKILRMAIPFSKEQVNEVQKQVVRANKLESCYIR
                     PLSWIGSQKLGVSPRGNQIHLMVAAWAWGAYLGEEGMKRGIRVKISSYTRHHVNITMT
                     QAKAVSNYTNSILANMEALDDGYDEAMLLDSAGFVSEGAGENLFVVKDGVIYTPDLSA
                     GALNGITRNTVFHICKDLGLEIVQKRITRDEIYIADEAFFTGTAAEVTPIRELDRVEI
                     GAGSRGPLTEKIQSAFFDIVNGRNPKYAHWLTKV"
     gene            6112..6336
                     /locus_tag="VGA59_17285"
     CDS             6112..6336
                     /locus_tag="VGA59_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX9721474.1"
                     /translation="MSGNPPNKEFVVELLARDLNANGGVFCPSPAAGMKLWNSHPRIY
                     LDVARTGEAKCAYCGTVYKLKEGEHFGGGH"
BASE COUNT         1111 a         2246 c         2277 g         1065 t
ORIGIN      
        1 cttcgggatg gccggggtcg gccacatgcc cgagcgacgc cgagaggtcc gccgggccga
       61 tgaagatgcc gtccacgccg tcgaccgccg cgatggcttc caggttgtcc agcgccatgc
      121 gcgtttccgc ctgcaccagc aggcagacct gcgcgttggc ttcattggcg tagttgggga
      181 agcggcccca gcgcgaggcc cgcgcgccgc ccatgccgcg gatgccgtcg ggcggatagc
      241 gcgtggcgcg cgcaaggtcg gcggcctgct gcgcggtgtc caccatcggc accagcaagg
      301 tctggatgcc caggtccagg tactgcttga tcagggcggt gccggcgtgg ccgtggccca
      361 ccgggacgcg cgcgatcgga tgcgtgccgg gatagcccgc gaccacctgc gcctgctgca
      421 ggagggtgcg caggtcattg ggcgcatgct cgccgtcgag cagcagccag tcgaagccgg
      481 cgccggcgca gatctccgtg ctgtagtgat ccgccagccc gacccacagg ccgatctggg
      541 ggcgcttttc acgcagcgcc tgcttgaagg agttgatggg caattgcatg ggaaaccttt
      601 agtgaaacgg aacgcggacg ggccggggcg gcaccagctt acccctggcc gcttccctcg
      661 ccatcctcca gcgataccgc ccgctgtggc gccgtgttca cccgcagcga gaagacgtcg
      721 ggccgcgcat agtggccgac cgggtcgaaa tccatctgtg ccatcgcgat cgccgacatg
      781 tcgagttccg ccgtgagcaa cgcatcctcg ttgtagaccg gcgcggccag caccttgccc
      841 agggggtcga tgatcatgct gccgccgcgc atcagcaccg tctccggcgc ttccggcaag
      901 cggttgttca tgcggtcgcc cggaaactgc gagcgtcgca aatgctggca ggccgacagc
      961 acgaaacagc gcccctccat cgcgatgtgc tgcatcgtcg aggcccaggt atcgcgatcg
     1021 tcggcggtcg gcgcacagta cagcgcgatg cgctgctggt acatggccat gcgcagcgcc
     1081 ggcatgtaat tttcccagca gatcaccgcg cccagcttgc cgtagggtgt gtccaccgcg
     1141 gccagcgtgg acccgtcgcc gaacccccag accaggcgct ccagcgccgt gggcatcagc
     1201 ttgcggtgct tgccgaggac ctgcccctgc gggccgaggt acacggcggt gcaatacagc
     1261 gtgccgccgt cgcgttcgat gatgccgacc accacgtaca tccggtgctg cgcggcagcc
     1321 ttggcgagct gcgcgatttc cgggccttcg acggcaaccg ccgcatcgaa gtagtgcttg
     1381 aattccgcgc ggccctgcgg cgtgcgcgcg cccaggtaga tgtggaagtc ggcgcccttg
     1441 gggtagccgc cgatgaaggc ctcgggaaac accaggagct gcacgtcgcg tcgagccgcc
     1501 tcgcccatca gtgcaagcgc gcgctccaca gtagcgggtg cgtccagcag gaccgaggcc
     1561 gtctgcgcga cagcggcggt gagagtcagt ttgtccatcg ctcactcctg acgtcccggt
     1621 ttcgaccggg gtgatttcgc cgtgaacggc cccattcttc ggttcgcaga atacctcaga
     1681 gcgccggggg gtgctcggct atagtcagcc ggccatggcc aaggaaaaaa ccagctacat
     1741 ctgcacggaa tgcggcggcg cgagcccgcg ctggctgggc aaatgccccc actgcgaggc
     1801 ctggaacacg ctgatcgaaa ccgtgccgga atctggcggg gccggccgca accgttttgc
     1861 ctccctggcg aagcctgccg aggtcgcggc gctggcggat atcgaggcca gcgagatcga
     1921 acgcacgccg accggcatcg gcgagctcga ccgcgtgctg ggcggcggca tcgtcgaggg
     1981 cggcgtggtg ctgatcggcg gcgaccccgg catcggcaag tccacgctgc tgctgcaggc
     2041 gctcgacagc ctccagcgct ccgcagtacc cacgctctac gtcaccggcg aggaaagcgg
     2101 ggcgcaggtg gcgctgcgct cgcgccggct cggcctggac aactcgcaag tgaaagtgct
     2161 ggccgagatc cagctcgaaa aaatcctcgc caccatcgcg gccgtccagc cggcggtggc
     2221 ggtgatcgat tcgatccaga ccgtttactc cgaccagctc acctcggcgc ccggctcggt
     2281 ggcccaggtg cgcgagtgcg cggcgcatct cacgcgcgcc gccaagtccg gcggcaccag
     2341 catcgtgctg gtcggccatg tcaccaagga aggcgcgctg gcggggccgc gcgtgctcga
     2401 gcatatggtt gacacggtgc tgtatttcga gggcgacacc cattccagct tccggctggt
     2461 gcgcgccgtc aagaaccgtt tcggcgccgt caacgagatc ggcgtgttcg ccatgacgga
     2521 gaagggactc aagggcgtgg ccaacccgag cgccatcttc ctgtcgcagc acagcgagcc
     2581 ggtaccgggc agctgcgtgc tggtgacgct ggagggcacg cgccccatgc tggtggaaat
     2641 ccaggcgctg gtggacagcg gcggcgtcag cccgcgccgg ctctcggtcg gcctggaccg
     2701 cgaccgcctc gccatgctgc tggcggtgct gcaccggcat gcgggcgtgg cctgcatgga
     2761 ccaggatgtg ttcgtcaacg ccgtcggcgg cgtgcgcatc agcgagccgg ccgctgactt
     2821 gtcggtgatg ttggcgatca cgtcgagcct gcgcggaaag ccgctgccca aggggttcat
     2881 cgcctttggc gaggtcggtc tggccggcga ggtccggccg gcgccgcgcg gccaggagcg
     2941 gctgcgcgaa gcggccaagc tgggcttttc cgtggccgtg gtgcccaggg ccaacgcgcc
     3001 caaacggggc agccgcgaga tcgagggctt gacaatccac cccgtcgagc ggatcgagga
     3061 ggcgatggac gtcgtgcgcg ggctggggta gaaaatagcg cccatgaacc tgcagcagat
     3121 cctcatgccg gtgctgggcg tggtcctcgt ggctgccgcc tggcatgtct acggctgggg
     3181 cggtgtcgcc atcgccgcag gtggcatcgt catgtggggg ctgctgcact tcacccggct
     3241 cacgcacgtg ctcagccgcg cggccgagcg gccggtcggc tacgtggcca gcgccgtgat
     3301 gctcaatgcc aagctcaagc ccggtgtcaa cctgctgcac gtgatcgcga tgaccagggc
     3361 gctgggcgag cggctgtcca aggaaggcga gcaaccggag ctctatcgct ggaccgacgg
     3421 cggcgcctcc agcgtcacct gcgaattcgc caatggccgg ctggtcaaat ggacgctggc
     3481 gcggccgccg gcgcaagcag acggcactta gagcgcccga gccgatgcct caccggctct
     3541 cgcgctcggc atccttctcg aaatcggcgt ccggcgcggc actggcgacc cagtcgggat
     3601 acatcgcggt gaagtccgct ttgatccgcg ccgccggcac gtcctcgaag ctgccgcgct
     3661 ggttgacgta ctccatgtga tggcggcctg cccggtcgta cgggtgatag agcgagtcgc
     3721 tgcggccatc ccaatccagc ggcaggatgc cgaaacgctc gcacagcgtc aggttgaagg
     3781 cgggcgtagc cttcacccag gcgccgtcca gccacatctc ggtgtagccg tgccagatgt
     3841 acagatccgt ccccgcgatc ttgcgcatgc gctcggtgga caggtggttg cgcacgtcgg
     3901 catagcccac ccgcgccgga atgcccgccg cccgcgacag cgcggccagc agcgccgctt
     3961 tcggcacgca ccagccataa ccggcctcga gcacccggct ggcgcgcagg ccggccacgc
     4021 tgaggtcgat gtggtagggg tcgtagcgga agccgtcgcg caccaggtag tacagagaaa
     4081 cggcgcgctg cctggcatcg gcgccggtgg cgcgttgcga gaactcgatc acggccgggt
     4141 gatcgctgtc gatcagcgtc gtaggcgcga gggtggccgg gccgggaatg tcatccatcg
     4201 aaaggtctcc acgttgcggt gactatacgg cttcgtccgc gccgtgccca gcggggggcg
     4261 gatgccccca cgcgcagtgc gccgccgggt ggcgcgggca taatcggcgc gcggccccaa
     4321 ccgtccgcca gaggagactt ttcgatgcct tgttacgcga tcgacggcgt cgtgccggtg
     4381 gtggacccca gcgcgttcgt tcatcccacc gcggtgctga tcggcgacgt catcgtcggg
     4441 cccaactgtt atgtcggccc ttgtgcctcg ctgcgcgggg acttcggccg catcgtgctc
     4501 ggcccgggcg cgaacgtgca ggacggctgc atcatccacg gctatcccaa gcaggacacg
     4561 ctggtggaag agaacggcca tatcgggcac ggagcggttc tgcacagctg cgtggtgcgc
     4621 cgcggcgcgc tggtcggcat ccgcgcggtg gtcatggacg atgtcgagat cggcgagcat
     4681 gccgtcgtgg ctgcgtgcgc cttcgtgccg gtgggaaggc acgtcgcccc gcgcacgatg
     4741 gtcgcgggaa ttccggccaa ggtggtgcgc acactcaagg gcgacaagga gatcgactgg
     4801 tatgcggaag gaacgcgggc ctaccacgag ctgaccggcc ggtgcctcgc ctccttgcgc
     4861 gaaacgcagc cgctggcggc cgtcgagccc gaccggctgc gcctgccggt gtccaaggtg
     4921 gacgccctga tcgtcgcccg ggatcgcggg atacgagaag cttaaaattc cagggtttca
     4981 cccctgatcg attaaaggaa gtctcatgat cgcacccccc tcgatggccg accgcgacgg
     5041 caagatctgg atggacggcg agctggtgga gtggcgcgac gccaaaatcc acgtgctgac
     5101 ccacacgctg cattacggat gcggcgtgtt cgagggcgtg cgggcctaca acacggtcaa
     5161 cggcaccgct gtcttccgcc tgcaggagca caccgatcgc ctgttcaata gcgccaagat
     5221 cctgaggatg gcgattccgt tctccaagga gcaggtcaac gaggtccaga aacaggtcgt
     5281 gcgcgccaac aagctggaga gctgctatat ccggccgctg tcgtggatcg gctcgcaaaa
     5341 attaggcgtc agcccgcgcg gcaaccagat ccacctgatg gtggcggcct gggcttgggg
     5401 cgcctacctg ggcgaggagg gcatgaagcg cggcatccgc gtcaagatct ccagctacac
     5461 ccgccaccac gtcaacatca cgatgacgca ggccaaggcg gtgagcaatt acaccaattc
     5521 catcctggcc aacatggaag cgctggacga cggctacgac gaggcgatgc tgctggacag
     5581 cgcgggtttc gtcagcgaag gggcgggcga gaacctcttc gtcgtcaagg acggcgtgat
     5641 ctacaccccc gacctgtcgg ccggcgcgct caacggcatc acccgcaaca ccgtgttcca
     5701 catctgcaag gacctggggc tggagatcgt gcaaaagcgc atcacgcgcg acgagatcta
     5761 catcgccgac gaggcgttct tcaccggcac cgccgccgag gtgactccga ttcgcgaact
     5821 cgatcgcgtc gagatcggcg ccggttcgcg cggccctctg accgagaaga tccagagcgc
     5881 ctttttcgac atcgtcaacg gccgcaatcc caaatacgcc cattggctca ccaaggtctg
     5941 aagtggaagc ccacccccga agcgccttcg gcgatgcccc ctcgggcttc gccctctccc
     6001 cctcgagggg acgccaccag cggcccggca tagccggttc cgcggtggcc cgcgcctgca
     6061 gaatcgactt tcatccgtcg tgggtgaagt tctccttcat gggaaactga tatgtccggt
     6121 aatccgccca acaaagaatt cgtggtcgaa ctgctggccc gcgacctcaa cgccaacggc
     6181 ggcgtgttct gccccagccc ggccgccggc atgaaactgt ggaacagcca tccgcgcatc
     6241 tacctggacg tcgcccgcac cggcgaggcc aagtgcgcgt attgcggcac ggtctacaag
     6301 ctcaaagagg gcgagcactt cggcggaggg cattgaccca ccccaggctc gtcatgccgg
     6361 gagtcgtcat gccggacttg atccggcatt catcgacagt ggcccaccag ccatggattg
     6421 cggggccggg ccgaagcgtg atgcctcggc acgtgcatag tgccggcccg gcattgggct
     6481 cgctgcactg gacagctgcg cgccccgggc gttccgcgag cccagcccgc aatgacagtt
     6541 cacccaacag ttgtcatgcc gggcttgacc cggcatccat gacttcttgg attgcgggtc
     6601 aagcccgcaa tgacagacct tgagcggctc actcgtgatc gccccgcagt ggatcggcga
     6661 cgcggtgatg accgagccgc tgctgcgccg gctgcgcac
//
LOCUS       DASWIY010000481         3816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_333375, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000481 DASWIY010000000
VERSION     DASWIY010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333375"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /gene="rfbB"
                     /locus_tag="VGA59_17290"
     CDS             <1..798
                     /gene="rfbB"
                     /locus_tag="VGA59_17290"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX9721475.1"
                     /translation="GPDAFIQTNIVGSFRLLEATRGYWSQLPSSATAGFRFLHVSTDE
                     VYGSLSPQAQAFTESHAYAPNSPYSASKAASDHLVRAYHHTYGLPVLTTNCSNNYGPY
                     HFPEKLIPLMIVNALAGKPLPIYGDGQQVRDWLYVGDHCSAIRRVIEAGRPGETYNIG
                     GWNEKPNIEIVHTLCDLLDELRPGAQGKSYRELITYVKDRPGHDRRYAIDARKIEREL
                     GWKPAETFESGIRKTILWYLENAHWVARVQDGSYREWLATNYAARAA"
     gene            805..1695
                     /gene="rfbD"
                     /locus_tag="VGA59_17295"
     CDS             805..1695
                     /gene="rfbD"
                     /locus_tag="VGA59_17295"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX9721476.1"
                     /translation="MKILLFGKGGQVGWELQRSLAPLGELVALDFDSQELCGNFTDLE
                     GLAATVEAVRPDVLVNAAAHTAVDKAESEPELAHTLNALAPGVLAQAAQALGSLMVHY
                     STDYVFDGSGRQRWKESDTPAPLNVYGRTKLEGERLVARHCPNHLIFRTSWVYAARGA
                     NFAKTMLRLAGERDKLTVIDDQFGAPTGAELLADVTAHAVRAVRRNAPLAGLYHLTAG
                     GETSWHGYARFVIEQAARSGVALKAGADAVQPVPTSAFPTPAERPRNSRLDTSRLEAA
                     FDLRLPAWQQGVSRMLTEIL"
     gene            1702..2589
                     /gene="rfbA"
                     /locus_tag="VGA59_17300"
     CDS             1702..2589
                     /gene="rfbA"
                     /locus_tag="VGA59_17300"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX9721477.1"
                     /translation="MTRRKGIILAGGSGTRLHPATLAISKQLLPVYDKPMVYYPLCTL
                     MLAGIRDILVISTPQDTPRFQQLLGDGSQWGLNLQYAVQPSPDGLAQAFIIGERFVGD
                     APSALVLGDNLFYGHDFAHLLAQADAQPEGATVFAYHVHDPRRYGVVAFDERGKASSI
                     EEKPAEPKSSFAVTGLYFYDRHVVEIAKSVKPSARGELEITSVNQVYLAQRSLNVQIM
                     ARGYAWLDTGTHDSLLEASQFIATLEHRQGLKIACPEEIAWRSGFITREQLELVAQQL
                     AKSGYGQYLLRLLHQKGFA"
     gene            2586..3140
                     /gene="rfbC"
                     /locus_tag="VGA59_17305"
     CDS             2586..3140
                     /gene="rfbC"
                     /locus_tag="VGA59_17305"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX9721478.1"
                     /translation="MKVTPTAIADVLLIEPRVFGDARGFFFESFNQKAFEQAVGRPAR
                     FVQDNHSSSVCGVLRGLHYQIRQPQGKLVRVVRGEVFDAAVDLRRSSATFGKWVGERL
                     SAENKRQLWIPEGFAHAFLVLSETAEFLYKTTAYYAPEYERCLIWDDPAVGVEWPDHG
                     LAGAPTLSAKDAAGRPLAQAETFD"
     gene            3146..>3816
                     /locus_tag="VGA59_17310"
     CDS             3146..>3816
                     /locus_tag="VGA59_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9721479.1"
                     /translation="MGPSREQVVGVVVTYFPDIGALRELLAAARPQVAALVVVDNTPV
                     TAPAGACCPPMDDVEIISLGFNAGLAAGLNHGCEWARRRGAGFALLFDQDSVPAADML
                     DRLAVAWTAAAASGLRVAAAGPRFSDSRGARVFPFLRVGMLRNRVVEPRPSDEFVRTD
                     ILITSGCLIALGALSDAGAMDESLFIDNVDLEWGFRARARGWALIGAPHAKLGHRIGD
                     EHVVAP"
BASE COUNT          650 a         1263 c         1262 g          641 t
ORIGIN      
        1 ggccccgacg ctttcatcca gaccaatatc gtcggcagct ttcgcctgct cgaggccacc
       61 cgcggctatt ggagccaatt gccctcgtct gccacggcgg ggtttcgctt cctgcatgta
      121 tcgaccgacg aggtgtatgg ctcgctgtcg ccgcaagctc aagccttcac cgaatcgcat
      181 gcttacgccc ccaacagtcc gtactccgcc agcaaggccg ccagcgacca cctggtgcgt
      241 gcctaccacc atacctacgg gctgcccgtt ctgacgacga actgctccaa caactatggg
      301 ccgtatcact tcccggaaaa gctgatcccc ctgatgatcg tcaacgcgct ggcgggcaag
      361 ccgctgccga tctacggcga tggccagcag gtgcgggact ggctctatgt gggagaccat
      421 tgcagcgcca tccgccgggt catcgaagcc ggccgacccg gcgagacgta caacatcggc
      481 ggatggaacg aaaaaccgaa catcgagatc gtccataccc tctgcgacct gctcgacgag
      541 ctgaggccgg gcgcccaagg caagagctac cgcgagctga tcacgtacgt gaaggaccgg
      601 ccgggccatg atcgccgcta tgcgatcgat gcgcgcaaga tcgagcgcga gctgggctgg
      661 aagccggcgg aaacattcga gagcggcatc cgcaagacca tcctgtggta cctcgagaac
      721 gcgcactggg tcgcccgcgt gcaagacggc agctacaggg aatggttggc tacgaactac
      781 gcggcccgcg ccgcctgagc ccccatgaag atattgttgt tcggcaaagg tggccaggtg
      841 ggctgggaac tgcaacgcag cctggcgccg ttgggcgagc ttgtcgcatt ggacttcgac
      901 agtcaggagc tgtgcggcaa cttcaccgat ctggaggggc tcgccgccac cgtggaagcg
      961 gtccggccgg acgttctggt caacgcggcg gcgcacaccg ccgtcgacaa ggccgagtcc
     1021 gagcccgaac tggcgcacac cctgaacgcc ctggcgccgg gggtcctggc gcaagccgcg
     1081 caagcgctgg gctcgctgat ggtccattac tccacggact atgtgttcga cggcagcggc
     1141 cgccagcgat ggaaggaatc ggacacgccg gcgccgctca atgtgtatgg gcgcaccaag
     1201 ctcgaaggcg agcggctggt cgcccggcat tgccccaacc acctgatttt tcgcaccagc
     1261 tgggtctatg cggcccgggg cgcaaacttc gccaagacca tgcttcgcct ggccggcgaa
     1321 cgggacaagc tcaccgtcat cgacgaccag ttcggcgcgc ccacgggcgc cgaactgctg
     1381 gccgacgtca cggcccatgc ggtgcgggcc gtccggcgca acgcgccact ggcggggttg
     1441 taccatctga ccgcgggcgg tgagacttcc tggcacggct atgcgcgttt cgtgatcgag
     1501 caggcggcac gcagcggagt cgccctcaag gccggcgccg atgctgtgca gccggtgccg
     1561 acctcggcct ttcccacgcc cgcggaacgt ccgcgcaatt cacggctcga tacgtccagg
     1621 ctcgaggcgg cgttcgacct gcgcctgccg gcatggcagc aaggcgtttc gcgtatgctg
     1681 accgaaattt tgtgaaccga tatgacacga cgtaagggca tcatcctggc gggcggctcc
     1741 ggtacccggc tgcatcccgc caccctggcg atcagcaagc agctcttgcc ggtgtacgac
     1801 aagccaatgg tgtactaccc cctgtgcacg ctgatgctcg cgggcatacg ggacattctg
     1861 gtgatcagca cgccgcagga cacgccccgg ttccagcagc tgctgggcga tggctcccag
     1921 tggggcctga acctccagta cgcggtccag cccagccccg acgggctggc gcaagccttc
     1981 atcatcggtg aacgcttcgt cggcgacgcg cccagcgcgc tggtgctggg cgacaacctc
     2041 ttctatgggc acgatttcgc ccacctgctg gcgcaagccg atgcccagcc ggaaggcgcc
     2101 acggtctttg cctaccatgt gcacgatccc cggcgctacg gcgtggttgc gttcgacgag
     2161 cggggcaagg ccagcagcat cgaggaaaag ccggccgagc ccaagagcag cttcgccgtg
     2221 acaggacttt atttctatga ccggcacgtg gtcgagatcg ccaagtccgt gaagcccagc
     2281 gcgcggggcg agctcgaaat cacgtcggtc aaccaggtgt acctggcaca gcgcagcctg
     2341 aatgtgcaga tcatggcgcg cggctacgca tggctcgaca ccggtacgca cgacagcctg
     2401 ctggaagcca gccagttcat cgccacgctg gagcaccggc aggggctgaa gatcgcgtgc
     2461 ccggaagaaa tcgcctggcg cagcggtttc atcacccgcg agcagctgga attggtggcg
     2521 caacagctgg ccaagagcgg ctacgggcag tacctcttgc gcttgctgca ccagaagggg
     2581 ttcgcttgaa ggtcacgccc accgcgattg ccgacgtgct tctgatcgag cccagggtct
     2641 tcggcgacgc gcgtggcttc tttttcgaga gcttcaacca gaaggcgttc gagcaagccg
     2701 tgggccgacc ggcgcggttc gtgcaggaca accattcctc cagtgtgtgc ggagtgctgc
     2761 gcggcttgca ctatcagatc cgccagcccc agggcaagct ggtgcgggtg gtgcgcggcg
     2821 aggtcttcga cgcggccgtg gacttgcggc ggtcctcagc cactttcggc aagtgggtcg
     2881 gcgagcggct cagtgccgag aacaagcgcc agttgtggat tcccgaagga ttcgcccacg
     2941 ccttcctggt cctgagcgaa acagccgagt tcctctacaa gacaaccgcc tactacgccc
     3001 ccgagtacga gcgctgcctg atctgggacg atccggcggt gggggtcgaa tggccggacc
     3061 acggtctcgc gggggcgccg acgctctcgg ccaaggacgc cgcgggccgc ccgctggcgc
     3121 aggccgaaac cttcgattga ggctcatggg tccgtcgcgg gaacaggtgg tcggcgtcgt
     3181 ggtcacctac ttccccgaca tcggggcctt gcgcgagctg ctcgccgcgg cccgtcctca
     3241 ggtcgccgca ctggtggtcg tggacaacac gcccgtgacc gctcctgccg gcgcgtgctg
     3301 cccgcccatg gacgacgtcg aaatcatctc gctcggcttc aatgccggcc tggccgcggg
     3361 attgaaccac ggctgcgaat gggcgcgccg gcgcggcgcc ggcttcgctc tgcttttcga
     3421 ccaggacagt gtgccggctg cggacatgct cgatcggctc gccgtcgcgt ggacggccgc
     3481 ggctgcgtcg gggcttcgag tcgcggcggc cggtccccga ttcagcgaca gccgcggagc
     3541 ccgcgtgttc ccctttctgc gggtcgggat gttgcgcaat cgggttgtcg agccgcggcc
     3601 ttcggatgaa tttgtccgca ccgacatcct gatcacttcg ggatgtctga tcgcgctggg
     3661 cgcgctcagc gatgcaggtg ccatggacga gagcctgttc atcgacaacg tggatctgga
     3721 atggggcttt cgtgcgcggg cgcggggttg ggcgctgatc ggcgcgccgc atgcgaagct
     3781 cgggcatcgc atcggggacg agcatgtcgt cgcgcc
//
LOCUS       DASWIY010000482         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_334889, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000482 DASWIY010000000
VERSION     DASWIY010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334889"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VGA59_17315"
     CDS             <1..1212
                     /locus_tag="VGA59_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9721480.1"
                     /translation="GAGRVFRENTNGGIVTGDGMALAFRHGVPLRDMEFVQYHPTCMP
                     GTGLLFTEACRGEGGFMLNKDGYRYLQDYGLGPAEPTPRNKAMELGPRDRLSQAFWHE
                     KQKGRTIKGPHGDVVHLDLRHLGEKKLRERLPLIYELAVEYLGVDPAKAPIPVLPAVH
                     YSMGGITADGNTAAPLPGLYSVGECSSVGIHGANRLGSNSLTELIVFGKVAGVQAAAY
                     SRTVPHGKEATLLKLAEDSQRRAMSVVARSDGTERIATLRREMARSMEDGCGIYRTAA
                     TLQSTCDKLAELKQRYKRVQVDDHSKGWNTDWLVAIELGYLIDVAEAMAHSALNRRES
                     RGSHQRLDGFEARDDVNFLRHSLANYRGADAPSITYGPVKITTSMPATRAYGAAGEEA
                     DRKAKEEAVNA"
     gene            1205..1957
                     /locus_tag="VGA59_17320"
     CDS             1205..1957
                     /locus_tag="VGA59_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX9721481.1"
                     /translation="MPESKRIEIQVLRYRPEQEDEPVWQSYYVPFTDDMSVLQGLQHI
                     KDDIDGTLSFRWSCRMAICGSCGMMVNGKPKLSCQTFLRDLLPGPVRIEALAHFPIER
                     DLVVNVEGFVKKLESVKPYIIPKKPRTLDQGEYLQTPEQLEQFEQYSSCINCMLCYAA
                     CPQFGLNSDFIGPGVLALAHRYNVDSRDGARDERMEIMSTEEGVWDCSAVGYCSVVCP
                     KHVDPANAVNQNKVNSAKDYFLRFLSPKGASK"
     gene            1954..2364
                     /locus_tag="VGA59_17325"
     CDS             1954..2364
                     /locus_tag="VGA59_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HEX9721482.1"
                     /translation="MNGKAGVRRPYVRPMQGWWRRDPFFMAYMLREATAFAVLVYSVV
                     LAVGVVRLAQGEAAWNGWLAALKTPGSILLHVVLLVSFAVHARSWFEIMPKTMPMMHF
                     GGRRVEEATITRTGWIATIIVSVVMFALTWWWKK"
     gene            2361..>2601
                     /gene="frdD"
                     /locus_tag="VGA59_17330"
     CDS             2361..>2601
                     /gene="frdD"
                     /locus_tag="VGA59_17330"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF003977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HEX9721483.1"
                     /translation="MKRSNAPIFWLLFGGGGMLAALFGTMLVFITGIAAPLAWPFGPE
                     LLSYPRVLAFGQNWIGKAFLFAIIALFAWHAAHRIK"
BASE COUNT          481 a          856 c          832 g          432 t
ORIGIN      
        1 ggcgccggcc gcgtgttccg cgagaacacc aacggcggca tcgtcaccgg cgacggcatg
       61 gcacttgcct tccgtcatgg cgtgccgctg cgcgacatgg agttcgtgca ataccacccc
      121 acctgcatgc cgggcaccgg cctgctgttc actgaggcgt gccgcggcga aggcggcttc
      181 atgctcaaca aggacggcta ccgctacctg caggactacg gactgggccc ggccgagccg
      241 acaccgcgca acaaggcgat ggagctgggc ccgcgcgacc gcttgagcca ggccttctgg
      301 cacgagaagc agaaaggccg cacgatcaag gggccgcacg gcgatgtggt tcatctcgac
      361 ttgcgccacc tgggcgaaaa gaaattgcgc gagcggctgc cgctgatcta tgagctggcg
      421 gtcgaatacc tcggcgtcga tcccgccaag gcacccattc ccgtgctgcc ggcggtccac
      481 tactccatgg gcggcatcac cgccgacggc aacacggccg cgccgctgcc aggcctgtac
      541 tcggtgggcg aatgctccag cgtcggtatc catggcgcca accggctcgg ttcgaattcg
      601 ctgaccgaac tgatcgtttt cggcaaggtc gcgggcgtcc aggccgccgc ctattccagg
      661 acggtgccgc acggcaagga agcaaccctg ctcaagctgg cggaggattc acagcggcgg
      721 gcgatgtccg tcgtggcacg ctccgatggc accgaacgca tcgccaccct gcgccgcgaa
      781 atggccagga gcatggaaga cggctgcggc atctaccgca ccgccgccac cttgcaatcg
      841 acctgcgaca agctcgccga gctgaagcag cgctacaagc gcgtgcaggt cgacgatcat
      901 tccaagggct ggaacaccga ctggctggtc gccatcgaac tgggctacct gatcgatgtg
      961 gcggaggcga tggcgcattc ggccctcaac cgccgcgaat cgcgcgggtc acaccagcgg
     1021 ctggacggct tcgaggcccg cgacgacgtg aacttcctga ggcactcgct cgccaactac
     1081 cgcggcgccg acgcgccgag catcacgtac ggcccggtga agatcaccac ctcgatgccc
     1141 gcgacgcgcg cctacggggc ggccggcgag gaagccgacc gcaaagcgaa ggaggaggct
     1201 gtcaatgcct gaaagcaaac gaatcgagat ccaggttctg cgctaccggc cggagcagga
     1261 agacgagccg gtgtggcaga gctattacgt cccgttcacc gacgacatgt cggtcctgca
     1321 gggactgcag cacatcaagg acgacatcga cggcaccctc agcttccgct ggtcctgccg
     1381 catggcgatc tgcggcagct gcggcatgat ggtcaacggc aagcccaagc tgtcgtgcca
     1441 gactttcctg cgcgacctgc tgcccgggcc ggtgcgcatc gaggcgctcg ctcacttccc
     1501 gatcgagcgg gaccttgtgg tgaacgtgga aggcttcgtc aagaagctcg agagcgtcaa
     1561 gccctacatc atccccaaga agccgcgtac gctggaccag ggcgaatacc tccagacgcc
     1621 cgagcagctc gaacagttcg agcaatacag ctcctgcatc aactgcatgc tgtgctacgc
     1681 ggcctgcccg cagttcggcc tgaactcgga cttcatcggt cccggtgtgc tggccctggc
     1741 gcaccgctac aacgtcgact cgcgcgatgg cgcgcgcgac gagcgcatgg aaatcatgag
     1801 caccgaggaa ggcgtgtggg attgctcggc cgtgggctac tgctccgtcg tctgtcccaa
     1861 gcacgtcgac cctgcgaacg ctgtgaacca gaacaaggtc aacagcgcga aggactattt
     1921 cctgagattc ctgtcaccga aaggggcctc caaatgaacg gcaaagccgg agtgaggcga
     1981 ccgtatgtcc ggccgatgca aggctggtgg cgccgcgatc cattcttcat ggcctacatg
     2041 ttgcgcgaag caaccgcgtt cgccgtgctt gtttactcgg tcgtgctggc cgtcggcgtt
     2101 gtgcgcctgg cacagggcga ggccgcctgg aacggctggc tggccgcgct caagacaccc
     2161 ggatcgatcc tgctgcacgt cgtgctgctg gtatcgttcg ccgtgcatgc ccgcagctgg
     2221 ttcgagatca tgccgaagac catgcccatg atgcacttcg gcggacgccg ggtggaagag
     2281 gcgaccatca cgcgaacggg ctggattgcc acgatcatcg tgtcggtcgt gatgttcgca
     2341 ttgacctggt ggtggaaaaa atgaagcgat cgaacgcacc catcttctgg cttctgttcg
     2401 gcggcggcgg catgctggcc gcgctgttcg gaaccatgct ggtgttcatc accggcatcg
     2461 ccgcgccgct ggcctggccc ttcggccccg agctgctgag ctatccgcgc gtgctcgcct
     2521 tcgggcagaa ctggatcggc aaggcattcc tcttcgcgat cattgcgctg ttcgcgtggc
     2581 atgccgcgca ccgcatcaag g
//
LOCUS       DASWIY010000483        10682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3638989, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000483 DASWIY010000000
VERSION     DASWIY010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 10682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10682
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3638989"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VGA59_17335"
     CDS             complement(<1..250)
                     /locus_tag="VGA59_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX9721484.1"
                     /translation="MKITTWNVNSLTARLQHVLDWTATNPVDVLCLQELKLTDDKFPL
                     EVLRSAGYEHCAVFGQKTYNGVAIMSRHPLRDVVKNIVG"
     gene            complement(247..750)
                     /locus_tag="VGA59_17340"
     CDS             complement(247..750)
                     /locus_tag="VGA59_17340"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX9721485.1"
                     /translation="MNLGLIYARARNGVIGKGGGLPWHLPEDMAHFKQVTMGCPVIMG
                     RKTWESLPERFRPLPGRTNIVVTRQPDWNAPGATRAASLPEAMALCAGESQAWVIGGA
                     EIYAQALPMAHIAEVTEIDADFEGDAFAPRFGPEWREVARQRQRSANGLDFSFVTYRH
                     TQHPGTA"
     gene            complement(755..1573)
                     /locus_tag="VGA59_17345"
     CDS             complement(755..1573)
                     /locus_tag="VGA59_17345"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX9721486.1"
                     /translation="MTAASRPVRSQYEDFMRHVYTHGTPKTDRTGTGTRSVFGYQMRF
                     DLAEGFPLVTTKKVHLKSIIYELLWFLQGSSSNNWLKERGVTIWDEWAKPNGDLGPVY
                     GVQWRSWPTPDGGHIDQIAQAIKTIKTNPDSRRIIVSAWNVADIPKMALAPCHAFFQF
                     YVADRKLSCQLYQRSADIFLGVPFNIASYALLTHMMAQQCDLDVGDFIWTGGDCHIYS
                     NHHEQVELQLSREPVAYPVLNIKRKPVSIFEYEYEDFEVQDYQCHAAIKAPVAV"
     gene            complement(1607..3019)
                     /locus_tag="VGA59_17350"
     CDS             complement(1607..3019)
                     /locus_tag="VGA59_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9721487.1"
                     /translation="MNPLIDRLRRIVGASHVLCEGDLSGWEQDWRKRARGKALAVVRP
                     GSTGEVAEVVKACAAAGTSIVAQGGNTGLVVGSIPDASGRQVVLSLQRLNAVPEVDAA
                     NLTITAQAGCVLQNLQQAAETAGFLFPLSLAAEGSCTIGGNLATNAGGTQVVRYGNAR
                     DLCLGLEVVTAQGEVWNGLSGLRKDNTGYNLRDLFIGSEGTLGVITAATMKLYPLPAA
                     TLTAWAAVPSLDDAVRLLGLAHKHLGASLTGFEVMGQYALGLVARHFRQMRVPLYEQV
                     PFCVLLENSDHESEMHARGRFEGLLETALEQGYATDAVVAENLTQAHQLWHIRESIPL
                     AQAEEGLNIKHDIAVPVSAIPEFCKVTDSLLAREIPGVRIVNFGHLGDGNLHYNLQAP
                     AGGDAAAFLLEQEERINTLVYDSVRRFGGSISAEHGVGSLKVEKLEHHKSPVALGLMR
                     SIKRALDPANLMNPGRVLRA"
     gene            complement(3016..3405)
                     /locus_tag="VGA59_17355"
     CDS             complement(3016..3405)
                     /locus_tag="VGA59_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEX9721488.1"
                     /translation="MNPSDSLTRADPGTAVALTRWLAVGSLLGLIALGLAWELWLAPL
                     RPGGSWMVVKVLPLCIPLAGLLRNRMYTYRWVSLMVWIYFTEGVVRAYSERGLSVRLA
                     LLEVALCLALFAACVLHVRIRLRKARS"
     gene            3430..4632
                     /locus_tag="VGA59_17360"
     CDS             3430..4632
                     /locus_tag="VGA59_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEX9721489.1"
                     /translation="MDFRPLLADLSRFPWKDTALTLGERFREDRLGLTASSLTFTTTI
                     ALVPFFTVALAVFTAFPMFGKLQGAVQAWLVQSLIPDAIARQVLGYLTQFAGKASRLG
                     AVGLAVLFITALALVLTIDRTLNSIWRVKRPRPLAQRVLIYWAVMTLGPLLLGASLSI
                     SSYVITASRGVVGSMPGVIKVLLDVIEFFLLAAGLAALYRYVPNTPVRSGHAWAGGLF
                     AATGIELARKLLALYIGKVPTYSAVYGTFATVPILLVWIYVAWVVVLFGAVIAAYLPS
                     LVAGTRRRGGGHGWQFQLALEVLRELHRARTSARRGLSAPELCAALELDSLQLEPVLE
                     TLVALDWIGRINEVEDEAQTRYILLADVQSTALEPLMRHLLLPHSEATAKLWKSGQLS
                     SVYLKDVV"
     gene            complement(4636..5454)
                     /locus_tag="VGA59_17365"
     CDS             complement(4636..5454)
                     /locus_tag="VGA59_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9721490.1"
                     /translation="MYFIVNGAKAFCYTGGKPFDAAKPTVVFIHGVLHDHSVWILQTR
                     YLAHHGWNVLAVDLPGHCRSEGDAPSSVEEAADFIAGLLDAAGVAKAALVGHSWGSLI
                     ALEAASRLKERVTHLVLVGVAYPMKVSAVLLDTSLNQPMKAIEMINIFERSTLAPPPS
                     GLGPGTWVYGTSMALNRRVLASNPRVNVFHRGFKACDSYANGEAAIAAVTCPVLFLLG
                     AQDQMTPRKAAQSLIGAARENGKRVKVVELPVGHHQMLETPDATLFAIHDFLRT"
     gene            5683..6387
                     /gene="urtE"
                     /locus_tag="VGA59_17370"
     CDS             5683..6387
                     /gene="urtE"
                     /locus_tag="VGA59_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEX9721491.1"
                     /translation="MLSVKNINQYYGGSHILRDVSLDVHPGRVTVLLGRNGVGKTTLL
                     KSLMGLVPIRTGSIQFDGKPIHGATPYQRARAGIGFVPQGREIFARLTVEENLRMGLA
                     SKSAATPIPGELYELFPVLKAMLGRRGGDLSGGQQQQLAIARALAARPRLLILDEPTE
                     GIQPSIIKDIGRVIRGLADQGLNGEKVAILLVEQYYDFARELADNYLVMERGEFIARG
                     QGKDMESRGVRQLVAI"
     gene            complement(6598..6819)
                     /locus_tag="VGA59_17375"
     CDS             complement(6598..6819)
                     /locus_tag="VGA59_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721492.1"
                     /translation="MSDQSPAKMPKTPRAEHGTPTEVSWGDGTGRQPYANRGPEEAEQ
                     TTGGEASAGDRGELSGRNLEQLEQVRKKP"
     gene            complement(6816..7532)
                     /locus_tag="VGA59_17380"
     CDS             complement(6816..7532)
                     /locus_tag="VGA59_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721493.1"
                     /translation="MFQRTSSTGPAGPVFYTSLLAALIGFGLFFDHVAMVGPPAGDAV
                     ADLRGDLARERASPEVEHVARWAVDSRDHRGLPFIVVDKARARLFAFDAGGRLLASAP
                     VLLGASRGDGPAADAATPAGRFVAASGLPVHGDAIMWINSDAALSLHGIPSQTSPGRA
                     AQRLASGILEDKRISDGSLHVAGQFYRDYLRPLQGQASIAYVLPEVHPARDVFNSVVT
                     GLGSGVAQSTRSQALARRPS"
     gene            complement(7638..8858)
                     /locus_tag="VGA59_17385"
     CDS             complement(7638..8858)
                     /locus_tag="VGA59_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEX9721494.1"
                     /translation="MTDQTFSASRWARALRWMGAVGIAALLAACGGGGGGGSADGTLR
                     VALTDASCLYDHVFVTVEKVGVHKSGTAADDDAGWSEIIISPPKQIDLRTLTNGVLEE
                     LGTTTLSAGQYSQVRLVLASNSGTGTTSLANSVQPAGGTLTALATPSGQQSGLKVQAH
                     FDVAAGQTTDLVLDFDACKSVVKAGNSGRYILKPVLTLVPPEGSGVQGFVSTALPLSS
                     TTVAAQQDGVTIRSTVPDSAGKFSIPFLPVGTYTLVITADGRATGVITGVPAVTATMV
                     INGTTTAIALPVSSMADVTGTVSVTSVSGSSTVSTALTDATVRALQPLTGGPTIEVAS
                     QPVDAVLGTYGLRLPVGAPVKAAYGAVLTFVPDATAAGKYTIQVQAPGRANLEAPADI
                     SAGASVGVNFAYGP"
     gene            complement(9075..9545)
                     /locus_tag="VGA59_17390"
     CDS             complement(9075..9545)
                     /locus_tag="VGA59_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721495.1"
                     /translation="MTATDPGRSRQSLVYFGVAALLAVTAATAQVATGTTGIDASGSY
                     QQEVQACMSGKTQQDQATCLKEARNAQADKKRGLLDNAGARFEANAQARCDVLTGEDK
                     AACQARMMGYGSTEGSVAGGGVLREVETVVMPAGSGSVTIQPQTSDPVVLVPSK"
     gene            complement(9693..10523)
                     /locus_tag="VGA59_17395"
     CDS             complement(9693..10523)
                     /locus_tag="VGA59_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX9721496.1"
                     /translation="MAWNARLALDYRLEGGRCVARFEHQGPLRILQTLYPEGDGVCHN
                     VLVHPPGGLVGGDTLEIAIRAAAGSHGLVTTPGATRFYRSLGEPAVQRTQIALDEDAR
                     LEWLPLEAICHSGCVAENHLGLALAPGAELIGWDVTALGLPESGLPFARGSLLQHLEL
                     RGGWLERGRIDALDERLMNGPLGLAGHRCIATLYLAAGSDLGRARREQALELARGVIV
                     AHALCATAGATAPGPRVVAVRVLAPLVEPALGLLKAIRDAWRPALWHLPATRPRTWAL
                     "
BASE COUNT         1732 a         3569 c         3555 g         1826 t
ORIGIN      
        1 agccgacgat gttcttcacg acgtcgcgta acgggtgacg gctcatgatg gccacgccgt
       61 tgtacgtctt ctggccgaac acggcacagt gctcgtagcc cgccgaccgc agcacctcga
      121 gcgggaactt gtcgtcggtc agcttgagct cctgcaggca cagcacatcg acggggttgg
      181 tggcggtcca gtccaggaca tgctgcaggc gcgccgtcag cgaattgacg ttccaggtgg
      241 tgatcttcat gcggtgcccg gatgctgcgt gtgccggtag gtgacgaaac tgaagtccag
      301 gccgttggcg gagcgctggc gctgtcgcgc cacttcccgc cactcgggcc cgaagcgggg
      361 ggcgaaggcg tcaccctcga agtccgcatc gatttcggtg acttccgcga tatgcgccat
      421 cggcagggcc tgggcataga tctcggcccc gccgatgacc caggcctgcg attcgcctgc
      481 gcataacgcc atggcctccg gcagcgacgc ggcgcgggtc gccccggggg cgttccagtc
      541 cggctgccgc gtcacgacga tgttggtccg cccgggcagc ggccggaagc gctcgggcag
      601 cgattcccaa gtcttgcgtc ccatgatgac cgggcagccc atcgtgacct gcttgaaatg
      661 cgccatgtcc tccggcaggt gccagggcag gccgcccccc ttgccgatca cgccgttgcg
      721 cgcgcgcgcg tagatcaatc ccagattcat gcgctcacac cgccacgggc gccttgatgg
      781 cggcgtggca ctggtagtcc tgcacttcga aatcctcgta ctcgtactcg aaaatcgaca
      841 cgggcttgcg cttgatgttc agcacggggt aggcgacggg ctcgcggctc aactgcagct
      901 ccacctgctc atggtggttg ctgtagatgt ggcaatcgcc gccggtccag atgaagtcgc
      961 caacatccag gtcgcactgc tgcgccatca tgtgcgtgag cagggcgtag ctggcgatgt
     1021 tgaacggcac gcccaggaaa atgtcggcgc tgcgctggta cagctgacac gagagttttc
     1081 ggtcggccac gtagaactgg aaaaaagcat ggcagggcgc cagcgccatc ttcgggatgt
     1141 ccgccacatt ccaggcgctg acgatgatgc ggcgcgaatc gggattggtc ttgatcgtct
     1201 tgatagcctg cgcgatctgg tcgatgtggc cgccgtcggg tgtcggccag ctgcgccact
     1261 gcacgccata gaccggacca aggtcgccgt tgggtttggc ccattcgtcc cagatggtga
     1321 cgccgcgttc cttcagccag ttgttgctgc tcgagccctg gaggaaccaa agcagctcgt
     1381 aaatgatgga cttgaggtgc acttttttgg tggtcaccag aggaaaacct tcggccaggt
     1441 cgaagcgcat ctggtagccg aacacgctgc gggtgccggt accggtgcgg tccgtcttgg
     1501 gcgtcccgtg cgtgtacacg tggcgcatga agtcctcgta ttgggagcga acggggcgcg
     1561 aagcggctgt catggatcgg attatcctca gatgctcaag aatcgttcag gcgcgcagca
     1621 cccggccggg gttcatcagg ttcgcgggat ccagtgcccg cttgatcgat cgcatcagcc
     1681 ccagcgccac cggcgacttg tggtgttcga gcttttcgac cttcaggctg ccgacgccat
     1741 gctcggccga aatcgagccg ccgaatcgcc ggaccgaatc gtagaccagc gtattgatcc
     1801 gctcttcctg ttcgagcagg aacgcagccg catcgccgcc cgccggcgcc tgcaggttgt
     1861 agtgcagatt gccgtcgccc aagtggccga aattcacgat gcgcacaccg gggatttcac
     1921 gcgccagcag gctgtcggtc accttgcaga attcaggaat cgccgatacc ggcaccgcga
     1981 tatcgtgctt gatgttcagc ccttcttcgg cctgggccag cggaatgctt tcgcggatgt
     2041 gccagagctg gtgtgcctgg gtgaggttct ccgcgaccac cgcatcggtc gcgtatcctt
     2101 gttccagcgc ggtctccagc aggccctcga accggccgcg ggcatgcatc tccgattcgt
     2161 ggtccgagtt ctccagcagc acgcagaaag gcacctgctc atataggggc acccgcatct
     2221 gccggaagtg cctggcgacc aggccgagcg cgtactggcc catcacttcg aaacccgtga
     2281 ggctcgcgcc caggtgcttg tgcgccaggc ccagcagccg caccgcatcg tccagggacg
     2341 gcacggcagc ccaagccgtc agcgtggcgg cgggcagggg atagagcttc atcgtggctg
     2401 ccgtgatgac gcccaatgtg ccttcgctgc cgatgaaaag atcgcgcagg ttgtagccgg
     2461 tgttgtcctt gcgcaggccc gagaggccgt tccagacttc gccttgcgcc gtgaccactt
     2521 ccaggcccag gcacaggtcg cgtgcgttgc cgtagcgcac gacctgcgtg ccgcccgcgt
     2581 tggtggcgag attgccgccg atggtgcaac tgccttcggc ggcaaggctc agcgggaaca
     2641 ggaaaccggc cgtctcggcc gcctgctgca ggttctgcag cacgcagcca gcctgcgcgg
     2701 tgatggtcag gttggcggcg tcgacctcgg gcacggcatt caggcgctgc aggctgagca
     2761 cgacctgccg gcctgaagcg tccggtatcg aacccaccac caggccggtg ttgccgcctt
     2821 gtgcgacgat ggacgtcccg gccgcggcgc aggccttcac cacctccgcg acctcgccgg
     2881 tggaaccggg ccgcaccacc gccagcgcct tgccgcgcgc gcgcttgcgc cagtcctgct
     2941 cccagccact gaggtcgccc tcgcacaaca cgtggctcgc gccgacgatc cggcgcaacc
     3001 ggtcaatcaa tggattcatg atctggcctt tcgcaggcga atcctcacgt gcagcacgca
     3061 ggctgcgaac agcgccaggc acagcgcgac ctcgagcagg gccaggcgca cgctcaagcc
     3121 acgttcgctg tacgcgcgca cgacaccctc ggtgaaataa atccagacca tcaagctgac
     3181 ccagcggtac gtatacatgc ggtttcgcag caggcccgcc aggggaatgc aaagcggcag
     3241 caccttcacg accatccagg agccgccggg ccgcagcggc gcgagccaca actcccaggc
     3301 cagccccaac gcgatcagcc ccagcaggct gcccacggcc agccagcggg tcaacgccac
     3361 ggcggtgccg gggtcggcac gggtgaggga atcggacggg ttcatggggc gatggcatca
     3421 tagcggccga tggacttccg cccgctgctc gccgatctct cgcgttttcc gtggaaggac
     3481 acggcgttga cgctgggcga gcggtttcgc gaagaccggc tgggcctgac ggccagcagc
     3541 ctgactttca cgaccacgat cgcgctcgtg cccttcttca cggtggcgct ggcggtgttc
     3601 acggcattcc cgatgttcgg caagctgcaa ggcgccgtgc aggcctggct ggtgcaaagc
     3661 ctgatcccgg acgcgatcgc ccggcaagtc ctgggctacc tcacgcagtt cgccggcaag
     3721 gcgagccggc tcggggccgt gggactggcg gtgctgttca tcaccgcgct ggccctggtg
     3781 ctgaccatcg accgcacgct caacagcatc tggcgggtca agcggcccag gccgctggcg
     3841 cagcgggtgc tgatctactg ggccgtcatg acactggggc cgctgttgct gggtgcttcc
     3901 ctgagcatca gctcgtatgt gatcacggct tcgcgcggcg tggtcggttc catgccggga
     3961 gtgatcaagg tgctgctcga cgtcatcgag tttttcctgc tcgcggcagg cctggcggcc
     4021 ttgtaccgat acgtcccgaa tacgccggtg cgcagcggcc acgcctgggc agggggcctg
     4081 ttcgccgcca cgggcatcga gctggcgagg aagctgctgg cgttgtatat cggcaaggtg
     4141 cccacctatt cggcggtgta cgggactttc gccacggttc ctatcctgct ggtgtggatt
     4201 tacgtggcct gggttgtcgt gcttttcggg gccgtgatcg cggcctacct ccccagcctg
     4261 gtggcgggca cacgccgccg cggtgggggg catggctggc aattccagct ggcgctggag
     4321 gtgctgcgcg aactgcaccg ggcacggacc tcggcccgcc gcggactgag tgcgcccgag
     4381 ctgtgcgccg cgctggaact ggactcgctg cagctggagc cggtgctgga gacgctggtg
     4441 gcgctggact ggatcggccg catcaacgaa gtcgaggacg aggcgcagac ccgctacatc
     4501 ctgctggccg acgtgcagtc gacggcgctg gagccgttga tgcggcatct gctgctgccg
     4561 cactccgagg cgaccgccaa gctctggaag agcggccagc tgtcgtcggt ttacctcaag
     4621 gacgtggtct aggactcatg tcctcaggaa atcatgaatg gcgaacagcg tcgcatccgg
     4681 cgtttccagc atctggtggt gacccacggg gagttccacg actttcacgc gtttgccgtt
     4741 ttcccgggct gcgccgatca gcgattgcgc ggccttgcgc ggcgtcatct ggtcctgcgc
     4801 gccgagcagg aacagcaccg ggcaagtgac ggcggcaatc gccgcttcgc cgttggcgta
     4861 actgtcgcaa gccttgaagc cgcggtggaa gacgttgacg cgcgggttgc tggccagcac
     4921 gcggcgattc agggccatgc tggtgccata cacccaggtg ccgggcccca gcccggaagg
     4981 cggcggggcc agcgtgctgc gctcgaagat gttgatcatc tcgatcgcct tcatcggctg
     5041 gttgagcgag gtgtccagca agaccgccga cactttcatt gggtaggcca cgcccaccag
     5101 caccaggtgc gtcacccgtt ccttcaggcg cgacgccgct tccaatgcga tcagggagcc
     5161 ccagctgtgt cccaccaggg cggctttggc gacgccggct gcgtccagca ggcccgcaat
     5221 gaaatccgcc gcctcctcga cgctggaagg cgcatcgccc tcgctgcggc aatggcccgg
     5281 aaggtccacc gccagcacgt tccagccgtg gtgcgccagg tagcgggtct gcaaaatcca
     5341 gacactgtgg tcgtggagca cgccgtggat gaagaccacg gtcggcttgg ccgcgtcgaa
     5401 gggcttgccg cccgtgtagc agaaggcctt ggccccgttc actatgaaat acatgcgctt
     5461 ttcctttgtt tgtcatgccg ggctcgaccc ggcatccatg aattcatgga ttgcggggcc
     5521 gggccggagc gtgatgcctc ggcacgtgca tagcgcaggc ccggcattgg gctcgctgca
     5581 ctggacagct gcgcgccccg ggcgttccgc gagcccagcc cgcaatgaca acctctgcgt
     5641 ttcatgcgtc gcgggtggcg cgaagtgcaa tggacacctg acatgctctc cgtcaagaac
     5701 atcaaccagt actacggcgg ctcgcacatc ctgcgggacg tgagcttgga cgtacacccc
     5761 ggcagagtca cagtgctgct ggggcgcaac ggcgtgggca agacgacgct gctcaagtcc
     5821 ctgatggggc tcgtgcccat caggaccggc agcatccagt tcgacggcaa gccgatccac
     5881 ggcgcgacgc cctatcagcg cgcgcgggcc ggcatcggct tcgtgccgca aggccgcgag
     5941 atcttcgcgc ggctcacggt ggaggaaaat ctgcgcatgg gcctggcctc gaaaagcgca
     6001 gccacgccga ttcccggcga gctgtacgag ctgttcccgg tgctcaaggc aatgctgggc
     6061 cggcgcggcg gcgatctgtc cggcggccag cagcagcagc tggccatcgc gcgggcactg
     6121 gcggcgcggc ccaggctgct gatcctcgac gagccgaccg agggcatcca gcccagcatc
     6181 atcaaggaca tcgggcgcgt catccgcggc ctggccgatc agggcctgaa tggcgaaaag
     6241 gtggcgatcc tgctggtcga gcagtactac gacttcgcgc gcgaactggc ggacaactac
     6301 ctggtgatgg agcgcggcga gtttattgcg cgcggccagg gcaaggacat ggaatcgcga
     6361 ggcgtgcgcc aactggtggc gatctgacgc tcttgcgtaa acctccttca cgagtgtagg
     6421 caggtgccta ctggcattgg ggccggcgtc gcgcacgcgg gccgccgggg ccggtatacc
     6481 ttgagggcat cgccaacctt tggagaaatc aaatgcccgt cattctctgg cttctgggtg
     6541 tcccgctcgc cgtcgtcatt ctgctgatgc tgttcggcgt gttctgaccc ggccggccta
     6601 cggttttttc ctgacctgct cgagctgctc gaggttgcgc cccgacagct cgccgcggtc
     6661 gccggcgctg gcctcaccgc cggtcgtctg ctccgcctct tccggtcccc ggttggcgta
     6721 gggctggcgc cccgtgccgt cgccccagct gacttcagtg ggcgtgccgt gttccgcccg
     6781 gggcgtcttg ggcatctttg ccggtgattg atcgctcatg agggtctcct ggccaatgct
     6841 tgtgaccgcg ttgattgcgc aacgccggac ccgaggcccg tgacgaccga attgaacacg
     6901 tcgcgcgcgg gatgcacttc tggcagcacg taggcaatgc tggcctgacc ctgcagcggg
     6961 cgcaggtaat cgcgatagaa ctgccccgcc acatgcagcg agccgtccga gatccgcttg
     7021 tcctcgagga tcccggaggc caatctttgc gcggcccggc ccggtgacgt ctgcgatgga
     7081 atcccatgca gggaaagcgc ggcgtccgag ttgatccaca tgatcgcgtc cccgtggacc
     7141 ggcagcccgc tggcggccac gaatcggccg gcgggtgtgg cggcatcggc ggccggcccg
     7201 tcgccgcggc tggcgcccag cagcaccggc gcgctcgcca gcagacgccc gcccgcgtcg
     7261 aacgcgaaca gcctggccct ggccttgtcc acgacgatga aaggcaggcc gcggtgatcc
     7321 cgcgaatcga cagcccaccg cgcgacgtgc tccacttcgg gtgaggcccg ctcgcgcgcg
     7381 agatcgccac gcaggtctgc gacggcatcg cccgcaggcg ggccgaccat cgcgacatgg
     7441 tcgaaaaaga ggccgaagcc gatcagggcg gcaagcaggc tggtgtagaa aaccggtccc
     7501 gcaggaccgg tgctggaagt tcgctgaaac acggtcgctc tagggtagcg gcgaccgctg
     7561 acccgtttgt aggacgaggc cgcgtgtaca tgctgttgca gcccgtcccc gccctttggc
     7621 gaactaccgc cggctcgtca tggcccgtag gcgaagttca cgccgaccga cgctccagcg
     7681 ctgatatccg ccggcgcctc caggttcgcg cggcccggtg cctggacttg gatcgtgtac
     7741 ttcccggccg ccgtggcgtc gggcacgaag gtcagcactg cgccataagc ggccttcacg
     7801 ggcgcgccca ccggcaggcg caagccataa gtgccgagga cggcgtccac tggctggctg
     7861 gccacctcga tggtgggccc gccagtcaac ggctggagcg cgcgcacggt tgcgtcggtc
     7921 agtgccgtgg aaaccgtgct gctgcccgaa accgatgtca cgctcactgt tcccgtcaca
     7981 tccgccatgc tggacaccgg caaggcgatt gcggttgtcg tgccattgat caccatcgtg
     8041 gcggtgaccg cgggaacgcc ggtgatcacg ccggtggcgc gaccgtcagc ggtaatgacc
     8101 agcgtgtagg tgccgaccgg caggaacggg atgctgaact tgccggcgct gtcgggcacg
     8161 gtggacctga tcgtgacgcc gtcctgttgc gcggccaccg tcgtgctgct caagggaagt
     8221 gccgtggaga cgaagccctg cacaccggag ccctcaggcg gcaccaacgt caagacgggc
     8281 ttgaggatgt atcgcccgga gttgccggcc ttcacgaccg acttgcaggc gtcgaagtcg
     8341 agcaccaggt cggtcgtctg gccggccgcg acgtcgaaat gggcctgcac tttcagccca
     8401 ctttgctggc cgctgggcgt cgccagcgcg gtcaaggtcc caccggcggg ctggaccgag
     8461 ttggccaggc tggtggtccc ggtcccgctg ttgcttgcca gcaccagccg gacttgcgag
     8521 tactggcccg ccgacagcgt cgtggtgccc agttcttcca gcacgccatt agtcagcgtg
     8581 cgcaagtcta tctgtttcgg cggagaaatg atgatctcgc tccagccggc gtcgtcatcg
     8641 gctgccgttc cgctcttgtg gacgccgacc ttttcaacgg tgacgaagac gtgatcgtaa
     8701 aggcagctcg catccgtcag cgccacccgc agcgtgccgt cagcggaacc gccgccaccg
     8761 ccgccgccgc acgccgccag caaggcggcg attcccaccg cacccatcca gcgcagggcg
     8821 cgggcccagc ggctcgcgga aaatgtctgg tcagtcatct cgggcctttc gtgatatgca
     8881 agagagaccc catgctgacc atatgaatga actgaacgtt agcagacgtg acgaaaatgc
     8941 tgccgtgcct gccccccgac gacatcctta cattcgcgca gccttcagca acacctgtgt
     9001 gttgctccgc ggcagcgtcc agacaaaaaa atagcgggcg cggggcccgc tatcaattgc
     9061 cgatcggccg gcgcttactt cgatggcacc agcaccactg gatcggaggt ctggggctgg
     9121 atggtcactg atccggagcc ggcgggcatc acgacggttt ccacctcccg cagcacgccg
     9181 ccgccggcga cgctgccctc ggtgctgcca tagcccatca tgcgagcctg gcaagcagcc
     9241 ttgtcctcgc ccgtcaagac atcgcagcgg gcctgggcat tggcttcgaa ccttgcaccg
     9301 gcgttgtcga gcaagccgcg tttcttgtcc gcttgcgcgt tgcgcgcctc cttcaggcaa
     9361 gtggcctggt cctgctgggt cttgccggac atgcaggcct ggacttcctg ctggtagctg
     9421 ccggaggcat cgatgcccgt ggtgcccgtg gcaacctggg cggtagcggc cgtcaccgcg
     9481 agcagcgcgg ccacgccgaa gtacacaagg ctttggcgcg agcggcctgg atcggttgcg
     9541 gtcatgttgg gatctccttg caattcctgg atgaatcaag tctaggcagg gcgccagcgc
     9601 ggccgtgccg gagagcagtc cgcattgggg gaggcctgat gccggtcggg gcgtaggacg
     9661 cggattgcag gcgggggact acaggctgtg gcctagagcg cccaggtgcg cggtcgggtc
     9721 gccggcaggt gccacagcgc cggcctccat gcgtcgcgga tggccttcag caggcccagc
     9781 gcgggttcca ccagcggcgc cagcacacgc acggcgacca cgcgcggacc cggcgccgtg
     9841 gcccccgccg tggcgcacaa ggcatgagcc acgatcacgc ctcgtgccag ctccagcgcc
     9901 tgctcccgcc gggcccggcc caggtcgctg cccgcggcca ggtacagcgt ggcgatgcac
     9961 cgatgacccg ccaggcccag cgggccattc atgaggcgct cgtccagcgc atcgatgcgg
    10021 ccgcgttcca gccaccctcc gcgcagttcc aggtgctgca gcaggctgcc tcgcgcgaac
    10081 ggcaacccgg actccggcaa ccccagcgcc gtcacgtccc acccgatcaa ctcggcgcct
    10141 ggtgcgagcg ccagcccgag gtggttttca gcgacacagc cgctgtggca aatcgcctcg
    10201 agtggcagcc actccagccg cgcgtcttca tcgagggcga tttgcgtgcg ctgcactgcc
    10261 ggctcgccca gcgagcgata gaagcgggtc gcgcccggcg tggtgaccag cccatgcgac
    10321 ccggctgcgg cgcgaatggc gatctccagc gtgtcaccgc cgaccaggcc gccgggcggg
    10381 tgcaccagca cgttgtggca gacgccgtcg ccctcgggat aaagcgtttg cagaatgcgc
    10441 agaggcccct gatgttcgaa gcgcgccacg cagcggccgc cttcgagccg gtagtcgagc
    10501 gccagcctcg cgttccaggc catctcaagc gcccggcgcg ccgcgcgccg ccccgtccct
    10561 ccggccgatc aagcagcgtg gcacgctgtc cagcccgagc tcgagcctgc cgaggccggc
    10621 acagatgctg aagaaggacg ctccaggcac aggaaagacg gaaaaacgca tatgaaccaa
    10681 cg
//
LOCUS       DASWIY010000484         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3640301, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000484 DASWIY010000000
VERSION     DASWIY010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3640301"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VGA59_17400"
     CDS             complement(<1..290)
                     /locus_tag="VGA59_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721497.1"
                     /translation="MGTYVSLHGRAFGFDSVTGALQRPGFDTLNDAEGVTAATTASNT
                     KFRGVTTLGSTAAKDYTMNAPAAAGLPATLTTISTSTAARTVSLASGNFQST"
     gene            complement(375..896)
                     /locus_tag="VGA59_17405"
     CDS             complement(375..896)
                     /locus_tag="VGA59_17405"
                     /inference="COORDINATES: protein motif:HMM:NF015550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEX9721498.1"
                     /translation="MTEKKITAKQLRFAEEYLIDLNATQAAIRAGYSKKTAEQYGYQL
                     LQKTSVQEAIASGRSKATEKAELSVAWVLDRLRTVAERCMQSEPVLGKRGEAILVETP
                     DGELAPAFTFQAAPANRSLELLGKHLGMFPDKVEHTGKGGGPIQTEELGDTERARRIA
                     FLLQQGLKQQTVQ"
     gene            complement(902..1240)
                     /locus_tag="VGA59_17410"
     CDS             complement(902..1240)
                     /locus_tag="VGA59_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721499.1"
                     /translation="MGNALRYKDGLGASDNDVIVETGDVSAYDEFMLMHTTAGALDVF
                     PSLDGTNYATAPLSLIDLGAATSDPVLVTSASRIFAFYGTYAKIRVLQNGAGVPVDVC
                     LICSRKGGAR"
     gene            complement(1279..2127)
                     /locus_tag="VGA59_17415"
     CDS             complement(1279..2127)
                     /locus_tag="VGA59_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721500.1"
                     /translation="MTIRAPLITPPPRRRAPQQQQVVCNLLLTTLAPVVQAPFSRTDW
                     PNPRVAARTLQPQVFSDLLQTTLAPAIAQAPFNQGEWPNPVCARPSMGRHVLYGISYF
                     PEADPPSTIKLDFQNPVIQRKVQQPQIFPNLVLGPLFVAPGVPFRQQEWPNPTVPRRQ
                     ARQDGAAGVRFRQLAPGPVLLGNIPNLSAGYDTGTHQYELGDYFSGATSYAIDPAVEV
                     GWTFNTTTGQLEIDTDAEGTFGPYIVTASAGDTTPSNAFTVKVSVSTIRPYRGIRGGG
                     GLRNYS"
BASE COUNT          396 a          682 c          743 g          485 t
ORIGIN      
        1 tccgtggact ggaagttgcc ggacgcgagc gacaccgtgc gcgccgcggt cgacgtgctg
       61 atcgtcgtga gcgtcgcagg caagcccgca gccgccggcg cgttcatcgt gtagtccttg
      121 gccgccgtgg agcccaatgt cgtcacgcca cggaacttgg tattactggc cgttgtggcg
      181 gctgtgacgc cttccgcgtc gttgagcgtg tcgaagccgg gccgctggag cgcgccggtc
      241 actgaatcaa agccgaatgc acggccgtgc agggaaacgt aagtgcccat gtggatctcc
      301 taaagaatgc ttgtgcggtt acccgctgct tgtggcgatc cttcgcctgc tgtgcgccgc
      361 tctgctggcg cgagctactg aacggtttgc tgcttgagcc cttgctgcag caggaaggca
      421 atgcggcgcg cacgctcggt gtcgcccagt tcttcggtct ggatcggtcc gccccctttg
      481 ccggtgtgct ccaccttgtc tgggaacatg cctaggtgct taccgagcag ttccagcgac
      541 cggttggcag gtgccgcctg aaatgtgaac gcgggcgcga gctccccatc gggggtctcg
      601 acgagaattg cctcacctcg cttgcccagc acgggctcgg actgcataca gcgctctgct
      661 accgtcctga gccgatccag cacccatgcg acgctcagtt cagccttttc ggttgccttg
      721 gagcggcctg aagcgattgc ctcttgcact gaagttttct gaaggagctg gtatccgtac
      781 tgctctgccg tcttcttgct gtaccccgcg cggatagcag cctgagtcgc attcaaatca
      841 atgagatact cttcggcgaa acgaagctgt ttggctgtta tttttttttc cgtcactagg
      901 cttaccttgc tccgcccttg cgcgagcaga tgaggcacac gtctacgggt acgccggcgc
      961 cattctgtag cacccgaatc ttggcgtagg tgccgtagaa cgcgaagatg cggcttgcag
     1021 aggtgaccag caccgggtcg ctcgtggccg cgcccaagtc gatgagggac agcggggcgg
     1081 tggcgtagtt ggtgccgtcc aaggacggga acacatccaa ggctccagcc gtggtgtgca
     1141 tgagcatgaa ttcgtcgtac gcggacacat ctccggtctc cacgatgacg tcattgtccg
     1201 atgcgccaag accatccttg taccgcaggg cattacccat gtggtcaggg actgctgctg
     1261 tgagtgccat gaattacctc aggaatagtt gcgcaatccg ccgccaccac gaatgccgcg
     1321 ataggggcga atggtggaga cggagacctt caccgtgaac gcattgctcg gtgtcgtatc
     1381 ccccgcggat gcggtgacga tgtacggccc gaaggtgccc tcggcatccg tgtcgatctc
     1441 gagctggccg gtggtggtgt tgaacgtcca gccaacctcg actgccggat cgatcgcgta
     1501 gctcgtcgca ccgctgaagt agtcgcccag ctcgtactgg tgggtcccgg tgtcgtagcc
     1561 cgccgagagg ttggggatgt tgccgagcag gaccggccct ggtgccagtt gccgaaagcg
     1621 cacccctgct gcgccatcct ggcgagcctg ccggcgcggc acggttgggt tcggccactc
     1681 ctgttggcgg aacggcacgc cgggagccac gaacagcggc cccaacacca gattcgggaa
     1741 aatctgcggc tgctggacct tgcgctggat gactggattc tgaaaatcca gcttgatcgt
     1801 tgagggcgga tcggcttccg ggaaatagct gatgccgtac aggacgtgac ggcccatgga
     1861 gggccgcgca cataccgggt tgggccactc gccctgattg aacggtgcct gcgcaattgc
     1921 tggtgcaagc gtcgtctgca gcaggtctga gaatacctgc ggttgcagag tgcgggctgc
     1981 tacacgtgga ttgggccagt ccgttcgact gaacggtgcc tgtactaccg gagcaagagt
     2041 cgtgagcagc agattgcaga cgacctgctg ctgttgaggg gcacgccgtc ttggtggcgg
     2101 cgtgatgagg ggagcgcgaa tggtcatagg ttagccatac gcgcggcctt cagcgcgtgc
     2161 gagcgcgcga cgggcttgtt tcctggcgcg gttgccggga tcacggtcgt aggctcgctc
     2221 atagattccg ctcttgtctt gtgccataaa acccctataa aacaaggcga attatagcat
     2281 cagccccccg ctttttcaga gcgttt
//
LOCUS       DASWIY010000485         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1461166, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000485 DASWIY010000000
VERSION     DASWIY010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461166"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..771
                     /locus_tag="VGA59_17420"
     CDS             367..771
                     /locus_tag="VGA59_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9721501.1"
                     /translation="MELSALARSSDREEADRARALLLSLQGWSSFRIAEAFAVKADSV
                     RHWRWLFGREGVSGLRARKALGPAPVKARAALAVAEAVLSEEALATRPNWTLPRLRAE
                     IAARTGVQISTSRLSVVLRKKGAFAGGGRDTP"
     gene            768..1385
                     /locus_tag="VGA59_17425"
     CDS             768..1385
                     /locus_tag="VGA59_17425"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX9721502.1"
                     /translation="MTGRQDADAVERAGVRLRLFKQQAEAGDIVLLFEDESEALTHPY
                     LSRVWAKRGADLRVEAPGQARKVAMLGVLDAASGELSVETSRTKRSSDFIALLERLDR
                     RYAPRAGQASKPVVVVIDNGPIHTSKASRAALDARPWLTAEWMPKYAPELNHIERSWR
                     DLKQHFLAHRTFQDADHLKAAIHTAVADLNRERQTNMCANLRIAA"
     gene            complement(1382..>1715)
                     /gene="yidC"
                     /locus_tag="VGA59_17430"
     CDS             complement(1382..>1715)
                     /gene="yidC"
                     /locus_tag="VGA59_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX9721503.1"
                     /translation="FGWIHDLSAPDPLLVFNLFGLIPWEPPQFLAIGIWPLLMGLTMY
                     LQQKLNPQPADPVQAKVFQFMPLFFVFLLGSFPAGLVIYWTWNNILSMAQQYVIMKRM
                     GVPIGGKT"
BASE COUNT          314 a          554 c          567 g          280 t
ORIGIN      
        1 ggcgatcggt cgcctgctta agaccaagcc gagcactgcc gccgccgccg ccggcgcgag
       61 ttcctggcct tcatgagcac cgcggtggca gcctttcggg gccgcgccct ttcgctggac
      121 caaggccgaa gtccatcaga agaacctcaa gccccgtatc gaacatttgc gagtccgggt
      181 actgggaagt cattgaggtc ccggcgcggc attcatgagc gttgcttata tgtccatacg
      241 catttcgcca tctaagcagg attcccaagg gtagtccaca tatggcagaa tttgtttcgt
      301 tgaccgctgg atatgggggt agccatggca ggtcgtcctc gagtggaggc gacgtcagag
      361 gagcgcttgg aactttccgc tctggcgcgc tcgtcggatc gcgaggaagc cgatcgggcg
      421 cgggcgctgc tgctgtcgct gcagggttgg tcgagctttc ggatcgccga agccttcgcg
      481 gtcaaggcgg acagcgtgcg ccactggcgt tggttgtttg ggcgggaggg tgtttcgggg
      541 ctgcgtgccc gcaaggccct gggcccggcg ccggtgaagg cgcgggcggc gctggcggtg
      601 gccgaggcgg tgttgtcgga agaagcgttg gcgacgcggc cgaactggac cttgccgcgt
      661 ctgcgggccg agatcgcggc gcgaaccggg gtgcagatct cgacctcgcg gctcagcgtg
      721 gtgctgagga aaaagggggc tttcgctggc ggcggccgcg acacaccctg accggccgtc
      781 aggacgccga cgccgtcgag cgcgccggcg tgcggctgcg cctgttcaag cagcaggccg
      841 aggccggtga catcgtgctg ctgttcgaag acgagtccga ggcgctcacc catccctatc
      901 tctcacgagt ctgggccaag cgcggtgccg acctgcgggt cgaagccccc ggtcaggcgc
      961 gcaaggtcgc gatgctcggc gtcctcgacg ccgcgagcgg cgaactcagc gtcgagacca
     1021 gccgaaccaa gcgcagcagc gacttcatcg ccctgctgga acggctcgat cgacgatacg
     1081 ccccgcgggc cggccaagcc agcaagccgg tcgtcgtcgt catcgacaac ggaccgatcc
     1141 ataccagcaa ggcatcccgc gccgccctcg acgcccggcc gtggctgacc gccgaatgga
     1201 tgccgaaata cgccccggaa ctgaaccaca tcgagcgctc ctggcgcgat ctcaaacagc
     1261 actttctcgc ccatcgcacc ttccaagacg ccgatcacct caaagccgcc atccataccg
     1321 ccgtcgccga cctcaacagg gagcgccaaa ccaatatgtg tgcaaacctg cgaatcgctg
     1381 cttaggtctt gcccccgatc ggcaccccca tgcgcttcat gatcacgtac tgctgcgcca
     1441 tcgagaggat gttgttccag gtccagtaga tcaccagccc ggccgggaag ctgcccagga
     1501 ggaagacgaa gaacagcggc atgaactgga acaccttggc ctgcaccggg tcggccggct
     1561 gcgggttcag cttctgctgc aggtacatgg taagccccat cagcaagggc cagatgccga
     1621 tcgccaggaa ttgcggcggc tcccagggaa tgaggccgaa caggttgaac accaacagcg
     1681 gatcgggcgc cgaaaggtcg tgaatccagc cgaag
//
LOCUS       DASWIY010000486         4033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1461664, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000486 DASWIY010000000
VERSION     DASWIY010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461664"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..764
                     /locus_tag="VGA59_17435"
     CDS             63..764
                     /locus_tag="VGA59_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX9721504.1"
                     /translation="MPDAATPAVRKLNRNIAIAGSVVLFHVAALWALQSGLLRRAVEI
                     VVPVQILSELVTPPAPKVEPPPAPPAPKPPQPLSKPTARKVERVVPPAPTPVAIPETR
                     PAPDAPTGVPAPQRPAPPVAAPVAPEPAPSGQAPAQAKVELPSSDARYLRNPAPVYPP
                     LSKRLGEQGKVVVRVLIGADGTPQKAELKRSSGYDRLDQSAVEYVMNCRYVPGKVDGV
                     PQSMWYEAPVSFVLE"
     gene            785..1498
                     /locus_tag="VGA59_17440"
     CDS             785..1498
                     /locus_tag="VGA59_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9721505.1"
                     /translation="MDTRFGLINLWSQGDFITKGVALALLAMSLSTWIVIIVKALDLR
                     KYAVQARLIESFWHAADFADGLAKLGPDPANPFRALAMEGREATAHHHAQPQLHDSLD
                     VSEWLTRSLRNSIDESTARLQSGLAVLASVGSTAPFVGLFGTVWGIYHALMSISAAGQ
                     ATIDKVAGPIGEALIMTALGLAVAIPAVLGYNALVRGNKAILNKLNRFSHDLHAYFVT
                     GARVSSSGDAKVVPMKSRS"
     gene            1555..1971
                     /locus_tag="VGA59_17445"
     CDS             1555..1971
                     /locus_tag="VGA59_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9721506.1"
                     /translation="MAFGTQDETDDVMNEINMTPLVDVMLVLLIIFIITVPVMKHSVD
                     IELPRASSRPQDAKPRTVRLTVDAQGVYFWNESRVDDTDLARMLKAEGARDPQPELHI
                     RGDKDVRYERVAQAMALAQQSGLRKIGFITQPKPGP"
     gene            complement(1984..2391)
                     /locus_tag="VGA59_17450"
     CDS             complement(1984..2391)
                     /locus_tag="VGA59_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX9721507.1"
                     /translation="MKTGSVLELADIKAVAAAAEAEALKNSWAVSIAIVDNGGHLLWL
                     QRLDGAAPISAHIAPAKASTAALGRRESKVYEEMINSGRVSFLSAPELKGLLEGGVPI
                     LKDGQCLGAVGVSGVKSAEDAQIARAGIAALGL"
     gene            2532..3218
                     /locus_tag="VGA59_17455"
     CDS             2532..3218
                     /locus_tag="VGA59_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX9721508.1"
                     /translation="MNDQVRTVDYGYALPAEQRNKVLRNTYWLLALSLVPTVLGAWMG
                     VATGITRGLSGGVGLMVFLGGAFAFMFAIEKTKNSAAGVPVLLAFTFFMGLMLARIIA
                     MVLGFKNGPSLIMTAFGGTAGVFFVMASLSSVIKRDLTGMGKFLFVGAVAILIGAIIN
                     VFVGSTVGMLVISVMAIGVFSAFMLYDLKRIIDGGETNYISATLALYLDIFNVFQSLL
                     ILLGLTSGDE"
     gene            complement(3280..>4033)
                     /locus_tag="VGA59_17460"
     CDS             complement(3280..>4033)
                     /locus_tag="VGA59_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase"
                     /protein_id="HEX9721509.1"
                     /translation="LRHLEPLSPGDVARLRAFAAAHPGVQWWLQPKGPDTVTLLDEGG
                     PQLAYELSEFGITMPFKPTDFTQVNPHINRVLVSRALRLLDPRKDERVIDWFCGLGNF
                     TLPIATRAREVLGIEGSETLVARSRENYERNNTPARPLARTGFAAANLFEITAAQLVG
                     HGPAQRWLVDPPREGAFALAKALADLHQQPELRAGWTAPKRIVYVSCNPATLARDAGL
                     LVHQGGYRCTAAGVVNMFPHTAHVESMAVFDL"
BASE COUNT          676 a         1358 c         1295 g          704 t
ORIGIN      
        1 gggtgctgcg ccgatagctc accgttgcgg ggcgtcccgt cccgggctcc tacactagcg
       61 gtttgcctga tgccgccact cccgccgtgc gcaagctcaa ccgtaatatc gccattgccg
      121 gcagcgtggt cctgttccat gtcgcggccc tgtgggcgct ccagtccggt ttgctgcgcc
      181 gcgcggtgga aatcgtcgtt ccggtccaga tcctgagcga actcgtcacg ccccccgcgc
      241 ccaaggtcga gccgcccccg gccccgcccg cacccaaacc gccgcagccc ctcagcaagc
      301 caactgccag gaaggtcgag cgcgtggttc cgcctgcgcc gacacccgtc gcgattcccg
      361 agacccggcc ggcacccgac gcgccgaccg gcgtccccgc gccccaacgc ccggcgccgc
      421 cggtagccgc cccggtggcg cccgagccag cgccgtccgg ccaggcgcca gcccaggcga
      481 aggtggaact cccctcctcc gacgcgcgct acctgcgcaa tcccgcgccg gtctatcccc
      541 ccctcagcaa gcggctgggt gagcagggca aggtcgtggt gcgcgtgctc atcggtgccg
      601 atgggacgcc gcagaaggcg gaactcaagc gttccagcgg ctatgaccgg ctggaccagt
      661 cagcggtcga atacgtcatg aactgccgct acgttcccgg caaggtcgac ggcgtgcccc
      721 aatccatgtg gtacgaggcg ccggtcagtt tcgttcttga ataatacgtt cgtggagtga
      781 cagcatggat actcgattcg gcctgatcaa tctctggagc cagggggatt tcatcaccaa
      841 gggcgtcgcg ctggcgctgc tcgcgatgtc gctgtcgacc tggatcgtga tcatcgtcaa
      901 ggcgctcgac ctgcgcaagt acgccgtgca agccaggctg atcgagagct tctggcacgc
      961 cgcggatttc gcggacgggc tcgccaagct cggccccgat ccagccaacc cgttccgcgc
     1021 gctggccatg gaaggccgtg aagcgaccgc gcaccaccat gcccagcccc agttgcacga
     1081 ttcgctcgac gtcagcgaat ggctgacgcg gtcgctgcgc aactcgatcg acgagtcgac
     1141 ggcccggctg cagtccgggc tggccgtgct ggcatcggtg ggttcgacgg cacccttcgt
     1201 cgggctgttc ggcactgttt ggggcatcta tcacgccttg atgtcgatca gcgccgccgg
     1261 gcaggccacc atcgacaagg tggcggggcc gatcggcgaa gccctgatca tgacggccct
     1321 gggcctggcc gtggccattc cggccgtgct gggctacaac gcgctggtgc gcggcaacaa
     1381 ggccatcctg aacaagctga accgcttctc ccacgacctc catgcctact tcgtcacggg
     1441 ggcgcgggtg tccagcagcg gcgacgcgaa ggtcgtcccg atgaaatcga ggagctagag
     1501 atgacatcac ccgctttggc ggcctcgccg ctcgactgcc tgacggagtt ctagatggct
     1561 ttcggaaccc aggacgaaac cgacgacgtg atgaacgaga tcaacatgac gccgctggtt
     1621 gacgtcatgc tcgtgctgct gatcatcttc atcatcaccg tgccggtcat gaaacactcg
     1681 gtcgacatcg aattgccgcg ggccagcagc cgcccccagg acgccaagcc gcgaaccgtg
     1741 cggctgacgg tggacgcgca aggggtttac ttctggaacg agagccgcgt cgacgacacg
     1801 gatctcgcgc gcatgctcaa ggctgagggc gccagggatc cgcagcccga gctgcacatc
     1861 cgcggcgaca aggacgtgcg ctacgagcgc gtggcccagg ccatggcgct ggcacagcaa
     1921 tcgggcctgc gcaagatcgg cttcatcacg cagcccaagc cgggcccctg agcccccgat
     1981 ggactacagg cccaacgccg caatgccggc gcgggcgatc tgcgcgtcct cggcggactt
     2041 gacgccgctt actccgacgg cgcccaggca ttggccgtcc ttgaggatcg gcacgccgcc
     2101 ctcgaggagg cccttcagtt cgggcgcgct caggaacgac acccgcccgc tattgatcat
     2161 ctcctcgtac accttgcttt cgcgccgtcc cagcgcggcc gtgctggcct tggccggcgc
     2221 gatatgggcg gaaatcggcg ccgcgccatc caggcgctgg agccaaagca ggtggccccc
     2281 gttatcgacg atcgcgattg atacggccca gctgttcttc agcgcctccg cttcggcggc
     2341 ggcggccacg gccttgatat cggccagttc gaggacggat cctgttttca tgagacggct
     2401 cgagaaaggg gaaaaaggta agcataacgg gtgccggacg atcccaaaaa ccgcccgttc
     2461 tggcgccgcg gccttgaacc aacctagaat gcgctcatct aaccgactca agcttaaagg
     2521 agagctggaa tatgaacgat caagtccgga ccgtcgatta cggctatgcc ctgccggccg
     2581 agcagcgcaa caaggtcctg cgaaacacct attggctgct ggccctgagc ctggtgccca
     2641 cggtactggg cgcctggatg ggtgtggcca ccggcatcac ccgcggcctt tcgggcggag
     2701 tcggcttgat ggtgttcctg ggtggcgcgt tcgccttcat gttcgccatc gagaagacga
     2761 aaaactcagc ggccggcgtg ccggtgctgc tggcgttcac cttcttcatg gggctgatgc
     2821 tcgcgcggat catcgccatg gtcctgggtt tcaagaacgg ccccagcctg atcatgacgg
     2881 ctttcggggg gacggccggc gtgttcttcg tgatggccag cctgtccagc gtgatcaagc
     2941 gcgacctgac gggaatgggc aagttcctgt tcgtgggcgc ggttgccatc ctgatcggcg
     3001 cgatcatcaa cgtcttcgtg ggttccacgg taggcatgct ggtgatcagc gtgatggcca
     3061 tcggtgtttt cagcgccttc atgctgtacg acctcaagcg gatcatcgac ggcggtgaaa
     3121 cgaactacat cagcgccacg ctggccctgt acctggacat cttcaacgtg ttccagagcc
     3181 tgctgatcct gctgggatta acgagcggcg acgagtaatc cgctacgctc aaggagtaaa
     3241 caagggggcc gatggccccc ttgtccttgt tggaggttgt cagaggtcga agacggccat
     3301 tgattccacg tgcgcggtgt gcgggaacat gttcaccacg ccggcggccg tacagcggta
     3361 gccgccctgg tgcaccagca atcccgcgtc tcgcgcgaga gtggccggat tgcagctcac
     3421 atagacgatg cgcttcggcg cagtccagcc ggcccgcagt tcgggttgct ggtggaggtc
     3481 cgccagcgcc ttggccaggg caaatgcccc ctcccgcggc ggatcgacaa gccagcgctg
     3541 ggccggccca tggccgacca gttgcgccgc cgtgatctcg aacagattgg cggcggcgaa
     3601 cccggttctt gccagcggcc gtgccggtgt gttgttgcgt tcgtagtttt cgcgcgagcg
     3661 ggccaccagc gtttcgctgc cttcgatgcc caggacctcg cgcgcccggg tagctatcgg
     3721 cagcgtgaaa ttgcccaggc cgcagaacca gtcgatcacc cgctcgtctt tccgcggatc
     3781 gagcagccgc aacgcgcgcg acaccaggac gcggttgatg tgcgggttca cctgggtgaa
     3841 gtcggtcggc ttgaacggca tcgtgatgcc gaattcgctc agctcgtaag ccagctgggg
     3901 cccgccttcg tccagcaggg tcaccgtgtc cggccccttg ggctgcagcc accactgcac
     3961 cccgggatgc gccgcggcaa aggcgcgcag cctggccaca tctcccgggc tcagcggctc
     4021 caggtggcgc agt
//
LOCUS       DASWIY010000487         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2945742, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000487 DASWIY010000000
VERSION     DASWIY010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2945742"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VGA59_17465"
     CDS             complement(<1..740)
                     /locus_tag="VGA59_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX9721510.1"
                     /translation="MAKKHVALSLFWRTFVLLAILLVGGVFAWTQTFRALELEPRAVQ
                     AAQQIANLVNLSRAALGSTDGINRVTLIKSMQQQEAMKLVPREPGDKWEPYEVGRSTR
                     AIGQELRSRLGPDTLVASELNGEPGLWVGFSFDKDPYWLQADPTRLGPMAWSTLFVWV
                     GIALLATVVGSVAIARLINQPLRDLSFAASRIREGEFDSRLDENTLTSEIRQVNMGFN
                     RMARELAKVEEDRAVMLAGISHDLRTPLA"
     gene            complement(835..1569)
                     /gene="ompR"
                     /locus_tag="VGA59_17470"
     CDS             complement(835..1569)
                     /gene="ompR"
                     /locus_tag="VGA59_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEX9721511.1"
                     /translation="MTTPQTARADRVVVVDDDARIRDLLRRYLTQEGFEVLLAEDAKA
                     LNRVLTRETVDLIVLDLMLPGEDGLSICRRLRAANDVTPIIMLTAKVEDVDRIVGLEV
                     GADDYLPKPFNPRELLARIHAVLRRRPAMEAPGAPAKDAMTVTFGPFEFDLALRRLSK
                     EGEQIALTTGEFSMLKALVRHPRQPLSRDKLAQLARGREFEPFDRSLDVQISRLRKMI
                     EPDPAQPRYIQTVWGVGYVFVPDGTA"
BASE COUNT          287 a          567 c          624 g          300 t
ORIGIN      
        1 gccagcggcg tgcgcaggtc gtgcgagatg ccggccagca tcacggcgcg gtcttcctcc
       61 accttggcga gttcgcgcgc catgcggttg aagcccatgt tgacctggcg gatctcgctg
      121 gtgagcgtgt tctcgtccag ccgcgaatcg aactcgccct cgcggatgcg gctggcggca
      181 aacgacaggt cgcgcagcgg ctggttgatg aggcgcgcaa tcgccaccga gccgaccacc
      241 gtggccagca gcgcgatgcc cacccacacg aacagcgtgc tccaagccat cggtcccaaa
      301 cgggtggggt cggcctgcag ccagtacggg tctttgtcga aggagaagcc tacccacagg
      361 cccggctcgc cgttgagttc gctggccacc agcgtgtcgg gccccaggcg cgagcgcagc
      421 tcctggccga tggcgcgggt ggagcggccc acctcgtagg gctcccactt gtcgcccggc
      481 tcgcgtggca ccagcttcat cgcctcctgc tgctgcatgc tcttgatgag cgtcacgcgg
      541 ttgatgccgt cggtgctgcc cagcgccgcg cgcgacaggt tcaccaggtt ggcgatctgc
      601 tgcgccgcct gcacggcacg cggctccagc tccagcgcgc gaaaggtctg cgtccaggcg
      661 aacacgccac ccaccagcag gatggccagc aggacgaagg tgcgccagaa caggctgagc
      721 gcgacgtgct ttttcgccat cgagcgggga cgcttgggag agtcgggggc tgcgggaatc
      781 tagcagacaa ggaaacggcc ccgcacaggg ggccgctcga tcgagggggt gtgttcaggc
      841 cgttccgtcg ggcacgaaca cgtagcccac gccccatacc gtctggatgt agcgcggctg
      901 tgccgggtcg ggctcgatca ttttgcgcag gcgcgagatc tgcacgtcca ggctgcggtc
      961 gaagggctcg aactcgcggc cgcgtgcgag ctgcgccagc ttgtcgcgcg acagcggctg
     1021 gcgcgggtga cgcaccagcg ccttcaacat cgagaactcg ccggtggtca gggcgatctg
     1081 ttcgccctcc ttggacaggc ggcgcagcgc caggtcgaac tcgaagggac cgaaggtcac
     1141 ggtcattgcg tccttggccg gtgcgcccgg tgcctccatg gcggggcggc ggcgcagcac
     1201 ggcatggatg cgcgccagca gctcgcgcgg gttgaagggc ttgggcaggt agtcgtcggc
     1261 gccgacttcg aggccgacga tgcggtccac gtcttccacc ttggcggtga gcatgatgat
     1321 gggcgtcacg tcgttggcgg cgcgcaggcg gcggcagatg gacaggccgt cttcgccggg
     1381 cagcatcagg tcgagcacga tgagatcgac ggtttcgcgc gtgagaacgc ggttcagggc
     1441 cttggcgtct tcggccagca ggacttcgaa tccctcttga gtgaggtagc gccgcagcaa
     1501 gtcgcgaatg cgggcgtcgt catcgacgac gacgacgcga tcggcgcgtg cggtctgagg
     1561 ggtggtcatg agcaactcca aatttgtaac agcgacattt tgcgcacgtt cacccacgga
     1621 atttagtgcc cctgaccagc tgttacaaat cttgctttgg ccccgcgggg aacgggattt
     1681 ggggctttgc ggggcactgg cagacccggc cgctgatacg ctggcgcgcc gcgccatccc
     1741 tttgacatca aaccggtggg tcttccgatg aaacagat
//
LOCUS       DASWIY010000488        11149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2945787, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000488 DASWIY010000000
VERSION     DASWIY010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2945787"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..192)
                     /locus_tag="VGA59_17475"
     tRNA            complement(116..192)
                     /locus_tag="VGA59_17475"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Met,seq:cat)
     gene            complement(230..1729)
                     /locus_tag="VGA59_17480"
     CDS             complement(230..1729)
                     /locus_tag="VGA59_17480"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9721512.1"
                     /translation="MKNRPPRQFVPLFVVQLLLAACSATAHSPPAPGVWPDPRPDLWP
                     NPMPIPTPVVRTVPNFQAPSATHCARPVQSREVPGRAGADQYSGSRHEYRDRLSKSAP
                     AEAAAAAPGAPGAPAAVPAPPPRAEAGRLADSASSGLAAPMFPSPQPQTQQPPARGPV
                     TAGMVDDNADFAGYLAFRNRTRVEHRPRDIRERYLLEVKDTRGRGIADAEVAVQAASG
                     HAMWARTDAAGKVWLHPNAFDPSRSAHYRVAVRKEGRHSSGYLARGQKSSVEIVLDAR
                     AAEPRARLDLVFLIDATGSMADEIDKLKATLRSISAEVARLPSQPDLCFGLVAYRDRG
                     DAFLLRSHDFTGDVGDFLRDALRPLRAAGGGDYAEAMNEAFHETVHNLSWRGDGATRM
                     VVLLADAPPHLDYGGPSYDEDMLAALGKGIKVFSVGASGLNRQGEFIQRQIAQYTGGR
                     FVFLTYAQAHDPASGPGRETVHDVKNYSVDTLDRLIVRLVSEELGKLAR"
     gene            1855..2469
                     /locus_tag="VGA59_17485"
     CDS             1855..2469
                     /locus_tag="VGA59_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9721513.1"
                     /translation="MPRVHSDMPDEELMLAYARGDAAAFDELYARHEGALFRFVRRLL
                     GTALAAQADEVFQDTWMRIIAARASFLPQGAAWRTWAFTIAHNLAMDRLRTSGREVGL
                     EPRDDGTDPLDWIASTLDMTHPSSEDVAFWRAAGRQLLHCLEELPDAQRAAFLLHHED
                     GASVEDLARRLGLQFETARSRLRYALQKLRGCMRRYLDVLEQPS"
     gene            2466..3590
                     /locus_tag="VGA59_17490"
     CDS             2466..3590
                     /locus_tag="VGA59_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721514.1"
                     /translation="MSDLRDARLRKALDCAPDADLRPDAKTRQAIHARARESAAPGGA
                     GPWWIGFWRGLAGQRMPWNAAFATFLLASLVTVLWYDREVPDARPETAPAEAPAAAGS
                     ALPSAPAPDQPAATASAPPEVRERAADAQQARKSAPTRPQPGLDSTRQAPPPGEPARG
                     RRDEPAVLSDRATGELARSAPQKLEKPGSDGTSAAPPPPAAAPTPMPAPAPAPAPAPA
                     AAPPAAPAAAPMAAPATAPAPAAATEAPAAPAAKARAPAAPAGLASQRPSPVESATHL
                     RIVGRGRIADAPLEHPSRLADLMGRVAREARSPEPLAAPADLRVELRRDGEPAGVLEL
                     GGPQVRWTPSRGVQDDGGTVRPDAAVLQALREELNRLLER"
     gene            complement(3602..4381)
                     /locus_tag="VGA59_17495"
     CDS             complement(3602..4381)
                     /locus_tag="VGA59_17495"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9721515.1"
                     /translation="MTHELIEVRTEGGKVGVVTLNRPKQLNALNSQLMDELGAALKTF
                     DADPAIGCMIITGSEKAFAAGADIGAMASWSFADVYRDDYITRNWETVRDIRKPVIAA
                     VGGFALGGGCELAMMCDFIIAADNAKFGQPEIKLGVIPGAGGTQRLPRAVGKAKAMDM
                     ILTARMMDAGEAERSGLVSRVVPLAKLMDEALGAALTICEFSQLSVMAAKESVNRAFQ
                     SGLSDGVMFERRMFHALFATADQKEGMDAFVNKRKAQFKHQ"
     gene            4500..5303
                     /locus_tag="VGA59_17500"
     CDS             4500..5303
                     /locus_tag="VGA59_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9721516.1"
                     /translation="MTEETVRFETRGAVALVTLNRPQSLNSFTRQMHRELWDALDRAE
                     ADKSIRALVLTGAGRGFCAGLDLSELDLKPGPNLVERANPGPVLEEAFNPTVRRLQAL
                     RVPTVAAVNGVAAGAGASLALTCDLAIAASGASFVQAFSKIGLIPDAGGSWFLVKRLG
                     LARALGCAMLGDKLSARDAKEQGLIWDVVADGEDCVEAAMKVAQRLAVLPTKALVATR
                     QLLRAAASRDLDAQLDAERDTQSALGRTHDYIEGVMAFLEKRPANFKGE"
     gene            5380..5805
                     /gene="paaI"
                     /locus_tag="VGA59_17505"
                     /pseudo
     CDS             5380..5805
                     /gene="paaI"
                     /locus_tag="VGA59_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106649.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
     gene            5982..7280
                     /gene="paaK"
                     /locus_tag="VGA59_17510"
     CDS             5982..7280
                     /gene="paaK"
                     /locus_tag="VGA59_17510"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HEX9721517.1"
                     /translation="MKSFPLEAIEKSGLDELRALQLKRLKSTLEHAYANSPVYRAKFN
                     AAGLRPGDCRSLADLAKFPFTTKKDLRDSYPFGMFAVPREQCVRLHASSGTTGKPTVV
                     GYTRGDIDTWADLMARSIRASGARPGDLVHVSYGYGLFTGGLGAHYGAERLGLTVVPF
                     GGGQTERQVQLINDFRPDIIMVTPSYMLAIADEFERQGLDPARSSLRLGIFGAEPWTN
                     DMRSAIERRMDMDAVDIYGLSEVMGPGVANECVETKDGPTIWEDHFYPEVIDPETGDV
                     LPEGQFGELVFTSLTKEALPIIRYRTRDLTRLLPGTARTMRRMEKITGRSDDMMIVRG
                     VNVFPTQIEELILKRSELTPHYQCILTREGPLDALTVAVETRQEVAHDSAAAQAAAQT
                     LAHEIKTYIGTSARIELRPCGGIERSLGKAKRVLDQRKTI"
     gene            complement(7368..8540)
                     /locus_tag="VGA59_17515"
     CDS             complement(7368..8540)
                     /locus_tag="VGA59_17515"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX9721518.1"
                     /translation="MVGGARLSAGAGTRLLALLAIVLLAHVLALQWLARQLEQPALLK
                     QMATPMFTRLLSAQAPVAVPAAPAAPAARARPRRPAITTLPAAKPPARAASQPVPAAD
                     PPAEAAPASAASEPATDIPVETAGESTAQPALPDTAVASAPEVDGAAPPMSDEPLTPA
                     APTTGATPAESGTESAALDNWPVDTRLNYRLAGHYRGELHGSARVQWQREGNRYQTRV
                     DIDINLLASLVLTSQGEVTMQGLLPRAYEELRRSGPRGVRLGDETITIANGRRLPRPE
                     GVQDTASQFVELSHRFAAGLETLEVGRSVSFWMARPGGLDHWTYDIVDREILYTPELG
                     AVEAFHLKPRTIVNPRGNITAEMWFAPSLQYLPVRIRVSMGDATYVDLMVDKIEQR"
     gene            complement(8519..10318)
                     /locus_tag="VGA59_17520"
     CDS             complement(8519..10318)
                     /locus_tag="VGA59_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEX9721519.1"
                     /translation="MTPAAAGRPPPRSASLHYQVEAADLHAHLFHVTLSIDQPAAQQR
                     VSLPVWIPGSYLVREFSKNLQRLTARQESRAVAVKQLDKCNWQIECVPSSPLVLSYEV
                     YAFDNSVRTAMLDTQRGFFNGTSLCLKVHGHESSVHSLELMAGKAIGHWEAATALAAH
                     KVGKRGFGTYLAVDYDELVDCPVEMGTFWSAEFKAGGVPHRLVVAGASEAFDSARLLA
                     DVHRICEAEVRFWHAHAGAAGRAGKRPPFKDYLFMLNAVDDGYGGLEHRSSTALIASR
                     RDLPRLGDARTSDGYVTLLGLISHEYFHTWNVKHLRPAEFTHYDYGGENYTQLLWFFE
                     GFTSYYDDLLLRRAGVIDDATYIKLLTKTINQVLQTPGREVQAVAQASFDAWVKYYRQ
                     DENTANATVSYYTKGALVALCFDLTLRAEGVTNLDEVMRALWQRCKAGPMTEADFAAV
                     LKELGGRAFTREIAAWIHGTRELPLEELLRPHGVVVLEEPAQLQQQLGLRLSETSGIQ
                     IKTVLRGGAAEQAGLAANDEWLGVEVAGAGWRMTKLDDLLLYAGNHKKVTVLVARDRR
                     LLRLELNLPASVTTWRLVLRDAARAQPWLAAHA"
     gene            complement(10315..11034)
                     /locus_tag="VGA59_17525"
     CDS             complement(10315..11034)
                     /locus_tag="VGA59_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEX9721520.1"
                     /translation="MHLIKKAVLAALLASGLFAGVAHAQEAAIRKNLPERLPQMGKID
                     EVSKTPMPGLFEVRVGTDVYYSDAEGNFLFHGQLIDTRQKRNLTEERQEKLLAIDFAA
                     LPVKDAFTIVRGNGSRKIAVFEDPNCGYCKRFERDLQKVDNITIHMFLYPILGADSAD
                     KSRNIWCARDKGKVWLDLMVRDQAAPSASCDTGALARNLQFGRKHKITGTPTLVFADG
                     TRVPGAIGAEQVEKLLAAGKP"
BASE COUNT         1745 a         3766 c         3819 g         1819 t
ORIGIN      
        1 atatggtgta cgggtggcgg taaacggcag gtattgtcgg aaataggctc ttggggaaag
       61 tcgagcttgc tatcgattgg atagcattat agatagttcc cttgcccggt gtgactggtg
      121 gcgcttcacg gactcgaacc gcggacctgt ggattatgat tccatcgctc taaccgactg
      181 agctaaagcg ccgagccttc cattatataa gagctgcgtc cggccggcct cagcgcgcca
      241 gtttccccaa ttcttccgac accagcctca cgatcagccg gtccagcgtg tcgaccgagt
      301 aatttttcac gtcatgcacg gtctcgcgcc ccgggccgct ggccgggtcg tgggcctggg
      361 cataggtgag gaacacgaaa cgcccgcccg tgtactgcgc gatctggcgc tggatgaatt
      421 cgccctgccg gttcaggccg ctggcgccga cgctgaacac cttgatgccc ttgcccagcg
      481 cggccagcat gtcctcgtcg taagacgggc cgccgtagtc caggtggggc ggcgcatcgg
      541 ccagcagcac caccatgcgg gtggcgccgt cgccgcgcca actgaggttg tgcacggttt
      601 cgtgaaaagc ctcgttcatg gcttcggcgt agtcccctcc gccggcggcc cgcaagggcc
      661 gaagcgcgtc ccgcaggaaa tcgcccacgt cgccggtgaa gtcgtggctg cgcagcagga
      721 aggcatcgcc ccggtcgcga taggccacca ggccgaagca caggtccggc tggctcggca
      781 gccgggccac ttccgccgaa atgctcctca aggtcgcctt cagcttgtcg atctcgtcgg
      841 ccatcgagcc ggtggcgtcg atcaggaaga ccagatccag cctggcgcgc ggctcggcgg
      901 ctcgcgcatc cagcacgatt tccaccgaac ttttctggcc gcgtgcgagg taccccgacg
      961 agtggcggcc ctccttgcgc acggccaccc ggtaatgggc ggagcgcgag ggatcgaagg
     1021 cattcgggtg cagccagacc ttgccggccg cgtcggtacg cgcccacatg gcatggccgc
     1081 tggccgcctg cacggcgact tcggcatcgg caatgccgcg tccgcgcgtg tccttcactt
     1141 ccagcaggta gcgctcgcgg atgtcgcggg gccggtgttc cacccgggtg cggttgcgaa
     1201 acgccaggta gccggcgaag tcggcgttgt cgtcgaccat gccggccgtg accggtccgc
     1261 gtgcgggcgg ctgctgagtt tgtggctgcg gcgaggggaa catcggcgcc gccaggccgc
     1321 tgcttgccga gtcggcgagc cgtcccgctt cggcgcgcgg cggcggcgcg ggtactgccg
     1381 cgggcgcgcc gggtgcgccg ggcgccgcgg ccgccgcttc agcgggagcc gacttggaca
     1441 ggcggtcgcg gtattcgtgg cggctgccgg aatactggtc cgcgcccgcg cggccgggaa
     1501 cctctcgcga ctggaccggc ctggcgcagt gcgtcgccga tggcgcctgg aagttcggca
     1561 cggtgcgcac gaccggcgtg gggatcggca tcggattcgg ccacaggtcc ggcctgggat
     1621 cgggccagac ccccggggcc ggtggcgaat gggccgtcgc gctgcaggcg gcgagcagca
     1681 gctgcacgac gaacagcggg acaaactggc gcgggggtcg gttcttcatc ggggcctcca
     1741 ggggttgtgg tgaaggctga tacggccggc cggcgcgggg agggtgactt tttctgcctg
     1801 ggtttaaccc cgaagcgcgc cctcgtccgt agcaggtgat gtgaaaatat gttcatgccc
     1861 cgcgtccaca gcgacatgcc cgacgaggaa ctgatgctgg cctatgcccg gggcgatgcc
     1921 gccgccttcg acgagctcta cgcgcgccac gaaggtgcgc tgtttcgttt cgtgcgcagg
     1981 ctgctgggta ccgcgctcgc ggcccaggcc gacgaggtgt tccaggacac ctggatgcgc
     2041 atcatcgccg cgcgcgccag cttccttccg cagggcgccg cgtggcgcac ctgggccttc
     2101 accatcgcgc acaacctggc gatggaccgc ctgcgcacga gcgggcgcga agtcgggttg
     2161 gagccgcggg acgacggcac ggatccgctg gactggatcg cctccacgct ggacatgacg
     2221 catccctcga gcgaagacgt ggccttctgg cgtgccgccg gccggcagtt gctgcattgc
     2281 ctggaggagt tgcccgacgc gcagcgcgcg gcgttcctgt tgcaccacga agacggggcc
     2341 agcgtcgagg acctggcgcg gcggctgggc ctccaattcg aaacggccag gagccggctg
     2401 cgctatgcgc tgcaaaagct gcgtggctgc atgcgccgct acctggacgt gctggagcag
     2461 ccgtcatgag cgatttgcgc gatgcgcgcc tgcgaaaggc gctggactgc gctcccgacg
     2521 cggatctgcg gcccgatgcg aagacgcggc aagccatcca cgcgcgcgcg cgcgaatcgg
     2581 cggcacccgg cggcgcgggt ccctggtgga tcgggttctg gcgtggactc gcgggccagc
     2641 gcatgccgtg gaacgcggcc ttcgccacct tcctgctcgc cagcctcgtc acggtgctgt
     2701 ggtacgaccg ggaggttccg gatgcgcgac ccgagactgc gccagccgag gcgcccgcgg
     2761 ccgccgggtc ggccctgccc agcgcgcccg cgccggatca gcctgcggcc acggcgtcgg
     2821 cgccgcccga agtgcgcgag cgggcagctg acgcacaaca ggctcgaaag agcgcgccca
     2881 cgcgcccgca acctgggctc gacagcacgc ggcaagcgcc gcctccgggc gagccggcac
     2941 gcggccggcg cgatgagccg gcggtgctga gcgaccgggc cactggtgag ttggccagaa
     3001 gcgcgccgca aaaacttgaa aagccggggt cggacggcac ctccgccgcg ccgcccccgc
     3061 cggcagccgc gccaacgcca atgcctgcac cagcaccagc acccgcacca gcaccagcag
     3121 ctgcaccacc agcagcacct gcggccgcgc cgatggcggc accggcaacg gcaccagcgc
     3181 ccgcggctgc gacagaggcg ccggctgcac ccgccgcgaa agcaagagcg cccgcagcgc
     3241 ccgccgggct ggcctcacag cggccctccc cggtggagag cgcgacgcat ttgcgtatcg
     3301 tgggccgggg tcgaatcgcc gacgccccgc ttgaacatcc ttcgcggctc gccgacttga
     3361 tgggccgcgt tgcgcgggag gcacgaagtc cggagccatt ggccgcgcct gccgaccttc
     3421 gcgtcgagtt gcgccgtgat ggcgagccgg cgggcgtgct ggaactgggc gggccccagg
     3481 tccgctggac gccgtcgcgg ggcgttcagg acgacggcgg taccgtgcga ccggacgcag
     3541 cggtgttgca ggcgctgcgc gaggaactca accgcctgct cgagcgttga actcccgcgc
     3601 gtcactggtg cttgaactgg gccttgcgct tgttgacgaa ggcgtccatg ccttccttct
     3661 gatcggccgt ggcgaacagg gcatggaaca tgcggcgctc gaacatcacg ccgtccgaga
     3721 ggccgctttg gaaagcgcgg ttgaccgact ccttggccgc catcaccgac agctgtgaaa
     3781 actcgcagat cgtcagcgcc gcgcccaggg cctcgtccat cagcttggcc agcggcacga
     3841 ccctgctgac caacccggac cgctcggcct ccccggcgtc catcattcgc gccgtcagga
     3901 tcatgtccat ggccttggcc ttgcccaccg cgcgcggcag gcgctgggta ccgccggcgc
     3961 ccgggatgac tcccagcttg atctcgggct ggccgaattt cgcgttgtcc gccgcgatga
     4021 tgaagtcgca catcatcgcc agctcgcagc ccccgcccag tgcgaatccg ccgacggcgg
     4081 cgatcacggg cttgcggatg tcgcggacgg tctcccagtt gcgggtgatg tagtcgtccc
     4141 ggtacacgtc ggcaaagctc cagctcgcca tcgcgccgat gtccgcgccg gcagcgaacg
     4201 ctttctcgct gcccgtgatg atcatgcagc cgatggcggg gtcggcgtcg aaggtcttca
     4261 gggccgcgcc cagctcgtcc atcagctggc tattcaatgc gttgagctgc ttgggccggt
     4321 tcagcgtgac gacgccgacc ttgccgcctt cggtgcgaac ctcgatcagt tcatgggtca
     4381 tgtgtgctcc tggatgaata tttcgactat accgaaggct tgcattcgcc gaccgatcgg
     4441 taaggttacg ggtacagtaa cgtcccggag accgctctca caccagcaca ggagcccgca
     4501 tgacagaaga aacggttcgc tttgaaaccc gtggcgcggt ggccttggtc acgctgaacc
     4561 gcccgcaatc gctcaacagc ttcacccggc agatgcaccg cgaattgtgg gacgcgctcg
     4621 atcgcgccga agccgacaaa tcgattcgcg ccctggtcct caccggcgcc gggcgtggct
     4681 tttgtgccgg cctggacctg tcggagctcg acttgaagcc ggggcccaac ctggtcgagc
     4741 gcgccaaccc ggggccggtg ctcgaggagg ccttcaatcc gacggtgcgc aggctgcagg
     4801 ccttgcgtgt gcccaccgtc gccgcggtca acggcgtcgc ggcgggtgcc ggcgcatcgc
     4861 tggcgttgac ctgcgacctg gccatcgccg cgtcgggcgc gtctttcgtc caggccttca
     4921 gcaagatcgg gctgatcccg gacgccggag gcagctggtt cctggtcaag cgcctgggtc
     4981 tcgcacgcgc cctgggatgc gcgatgctgg gcgacaagct cagtgcccgg gatgccaagg
     5041 agcaggggct gatctgggac gtcgttgccg acggcgagga ctgcgtcgaa gcggcgatga
     5101 aagtggcgca gcgcctggcc gtgctgccca cgaaagccct ggtggcgacc cgccagttgc
     5161 tgcgcgccgc cgcctcccgc gatctcgatg cgcaattgga tgccgaacgc gatacccagt
     5221 cggcgctggg ccggacgcat gactacatcg aaggcgtgat ggcattcctc gagaaacgcc
     5281 ccgcgaactt caagggggaa tgaatgcccc ccacgctcac ttttgttcgc tgccccccag
     5341 ggggcttcag cctccttgag gcggctcggc ggaggtgaga tgaacgaacg ggcgcttgcc
     5401 gccagggtcg gggaggcgat gttcgcggcc gaccgcgcca cgcgggactt catgcggatg
     5461 gaactggtca gctgcgagcc gggccgggcg gtgctgcgca tggtggtgcg cgaagccatg
     5521 ctcaatgggc acgacatctg ccatggcgga ctgatcttcg cgttggcgga ttcgaccttc
     5581 gcatacgcct gcaacagccg caacaaggcc accgtcgcct cgggatgcag catcgagttc
     5641 ctcaagcccg gccggcgggg cgatgtgctg acctgcgaag gtgccgagca ggtgctgaag
     5701 gggcggcacg gcatctacga catgaaggtg agcaaccagg caggcgaggt cgtggcgatg
     5761 ttccgcggcc gcagcacgca agtccagggc agcgtgatac cgctgccttc cgttcgagaa
     5821 gccgcaggcc cttcgccagg ctcaggacag ccggacggcc ttcttctccc tctccctcag
     5881 ggagagggcg ggggtgaggg tcatcagcgg accaagccct caccctaacc ctctcccaga
     5941 gggagagggg atagacacaa gctcaggacc acggagatac catgaaaagt tttcccctgg
     6001 aagcgatcga gaagtcgggc ctggacgaac tgcgggccct gcagctcaag cggctgaagt
     6061 ccacgctcga gcatgcctac gcgaactcgc cggtgtaccg cgccaagttc aatgccgcgg
     6121 ggttgcggcc gggggattgc cggtcgctcg cggacctggc gaaatttccc ttcaccacga
     6181 agaaggacct gcgcgacagc tacccgttcg gcatgttcgc cgtgccgcgc gagcagtgcg
     6241 tgcgccttca cgcctccagc ggcacgaccg gcaaaccgac cgtggtcggc tacacgcggg
     6301 gcgacatcga cacctgggcg gacctgatgg cgcgcagcat tcgcgcgtcc ggcgcgcgtc
     6361 cgggcgacct ggtgcacgtg agctacggct atgggctgtt caccggcggg ctgggggcgc
     6421 actatggcgc ggagcgcctg ggcctgacgg tggtgccgtt cggcggcggc cagaccgagc
     6481 gccaggtcca gctgatcaac gatttccggc ccgacatcat catggtcacg cccagctaca
     6541 tgctggccat cgcggacgaa ttcgaacgcc aggggctcga tcctgcaagg tccagcctgc
     6601 gcctgggcat cttcggcgcc gagccgtgga ccaacgacat gcgctcggcc atcgagcggc
     6661 gcatggacat ggatgccgtg gacatctacg gcctgtccga agtgatgggc cccggggtgg
     6721 ccaacgaatg cgtggagacc aaggacgggc cgacgatctg ggaggaccat ttctacccgg
     6781 aagtgatcga tccggaaacc ggtgacgtgc tgcccgaagg ccagttcggc gaactcgttt
     6841 tcaccagcct gaccaaggag gcgctgccga tcatccgtta ccgcacgcgc gacctcaccc
     6901 gcctgttgcc gggcaccgcc cgcaccatgc ggcgcatgga gaagatcacc ggccgcagcg
     6961 acgacatgat gatcgtgcgc ggcgtcaacg tcttccccac gcagatcgag gagctgatcc
     7021 tcaagcgcag cgaactgacg ccgcattatc agtgcatcct gacccgcgag gggccgctcg
     7081 atgcgttgac cgtcgccgtc gaaacgcgcc aggaggtggc gcacgactcc gcggcggcgc
     7141 aggccgcggc gcagaccctg gcgcatgaga tcaagaccta catcggcacc tcggcgcgca
     7201 tcgagctgcg cccctgcggc ggaatagagc gcagcctggg caaggccaag cgggtgctcg
     7261 accagcgtaa gacaatttag ggaatctgtc attgcgggct tgacccgcaa tccaggaccc
     7321 taattcatgg atcccggatc aagtccggga tgacagccag ggatcgatca gcgctgctcg
     7381 atcttgtcga ccatcaggtc gacgtaggtg gcgtcgccca tgctgacgcg gatgcgcacc
     7441 ggcaagtact gcaggctggg cgcgaaccac atctcggccg tgatgttgcc gcggggattg
     7501 acgatcgtcc gcggtttgag gtgaaaggct tccacggcgc ccagttccgg cgtgtaaagg
     7561 atctcgcggt ccacgatgtc gtaggtccag tgatcgagtc ctcccgggcg ggccatccag
     7621 aaactgacgc tgcggcccac ctccagagtc tccagcccgg cggcgaagcg atgactgagt
     7681 tccacgaact ggctcgccgt gtcctgcaca ccttccgggc gcggtaaccg gcggccgttc
     7741 gcgatggtga tcgtctcgtc acccaatcgc acgccccggg gaccactgcg gcgcaactcc
     7801 tcgtaggccc tgggcaatag cccctgcatc gtgacctcgc cctggctcgt cagcacgagg
     7861 ctcgccaata agttgatatc gatatcgacg cgcgtctggt agcggtttcc ctcgcgttgc
     7921 cactgcaccc gcgcgctgcc atgaagctcg ccgcggtagt gccccgcgag ccggtagttg
     7981 agccgggtgt cgacgggcca gttgtccagt gccgcggatt cggtgcccga ttctgcgggc
     8041 gttgcgcccg tggttggtgc ggcgggcgtg aggggttcgt cgctcatagg cggtgccgcg
     8101 ccgtcgacct cgggcgcgct tgccaccgcg gtgtccggga gcgccggctg cgccgttgac
     8161 tcgccggcag tttcgaccgg gatatccgtt gccggctcgc tcgctgccga ggcgggtgct
     8221 gcttcggccg ggggatcggc tgcgggaacg ggctgcgatg ccgcgcgcgc gggtggcttg
     8281 gcggccggga gcgtcgtgat ggcgggacgc cgcggccggg cgcgcgctgc cggcgccgcc
     8341 ggtgcggcgg gcacggcgac cggggcttgc gcactgagca gccgcgtgaa catcggagtg
     8401 gccatctgct tcaacagggc gggctgttcc agctggcggg cgagccactg cagcgccagc
     8461 acgtgcgcca gcagcacgat cgccaacagc gccagcagcc gcgtgccggc gccggcgctc
     8521 aggcgtgcgc cgccaaccat ggctgggccc gggccgcgtc gcgcaagacc aggcgccagg
     8581 tggtgaccga cgccggcagg ttcagctcca gccgcaacag ccgccggtcg cgcgccacca
     8641 gcaccgtgac cttcttgtga ttgccggcgt agagcagcag gtcgtccagc ttggtcatgc
     8701 gccagccggc cccggccact tcgaccccca gccattcatc gttggcggcc aagcccgcct
     8761 gctcggcggc gcctccccgc aatacggtct tgatctggat gcccgaggtt tcgctcaggc
     8821 gcaggcccag ctgctgctgc aactgggccg gctcttccag caccaccacg ccatggggcc
     8881 gcagcaactc ttccagcggc aattcgcgcg tgccgtggat ccaggccgcg atctcgcggg
     8941 tgaaggcccg cccgcccagt tccttgagca ccgccgcgaa gtcggcctcg gtcatcggcc
     9001 cggccttgca gcgctgccac agggcccgca tcacttcatc gagattggtg acgccctcgg
     9061 cgcgcagggt caggtcgaag cacagggcga ccagggctcc cttggtgtaa taactcacgg
     9121 tggcattggc ggtgttctcg tcctgccggt agtacttgac ccaggcgtcg aagctggcct
     9181 gggccaccgc ctgcacctcg cggccgggcg tctgcagcac ctggttgatg gtcttggtca
     9241 gcagcttgat gtaggtggcg tcgtcgatca cgccggcgcg gcgcagcagc agatcgtcgt
     9301 agtagctggt gaagccctcg aagaaccaca gcagctgggt gtagttctcg ccgccgtaat
     9361 cgtagtgcgt gaattcggcc ggccgcaggt gcttgacgtt ccaggtgtgg aaatactcgt
     9421 ggctgatcag gcccagcagg gtcacgtagc cgtcgctggt gcgggcgtcg cccagccgtg
     9481 gcaggtcgcg ccgcgaggcg atcagcgccg tcgagctgcg gtgctccagc ccgccgtagc
     9541 cgtcgtcgac ggcgttgagc atgaacaggt aatccttgaa cggcgggcgc ttgccggcgc
     9601 ggcccgccgc gcctgcatgt gcatgccaga agcgcacttc ggcctcgcag atcctgtgca
     9661 cgtcggccag cagtcgcgca ctgtcgaacg cctcgctggc gccggccacg accagtcgat
     9721 ggggcacgcc gccggccttg aactcggcgc tccagaatgt gcccatctcg accgggcaat
     9781 cgaccagctc gtcgtaatcg accgccaggt aggtgccgaa gccgcgcttg ccgaccttgt
     9841 gggcagccag cgccgtggcc gcctcccagt ggccgatcgc cttgcccgcc atcagctcca
     9901 gcgaatggac cgagctctca tgcccatgca ccttcaggca caggctggtg ccgttgaaaa
     9961 atccacgctg cgtgtccagc atggccgtgc gcaccgaatt gtcgaaagca tagacctcat
    10021 agctgagcac gagcgggctg ctggggacgc actcgatctg ccaattgcat ttgtcgagct
    10081 gcttgacggc gaccgcgcgg ctctcctgcc gcgcggtcag ccgctgcaga ttcttggaga
    10141 actcgcgcac caggtagctg ccgggaatcc acaccggcag cgacacccgc tgctgcgccg
    10201 ccggctggtc gatgctgagc gtgacgtgaa acaggtgggc gtgcaggtcg gccgcctcga
    10261 cctggtagtg gagactggcg ctgcggggcg gggggcgacc agcggccgcc ggcgtcatgg
    10321 ttttcctgcg gccagcagtt tctcgacctg ctccgcgccg atcgcgcccg gcacgcgcgt
    10381 tccgtcggcg aaaaccaggg tcggcgtgcc ggtgatcttg tgcttgcggc cgaattggag
    10441 attgcgcgcc agcgcgccgg tatcgcagct ggccgacggc gccgcctggt cgcgcaccat
    10501 caggtccagc cagaccttgc ccttgtcccg ggcgcaccag atgttgcgcg acttgtcggc
    10561 cgagtcggcg ccgaggatgg gatacaggaa catgtggatg gtgatgttgt cgactttctg
    10621 caggtcgcgc tcgaatcgct tgcagtagcc gcagttcggg tcctcgaaca cggcgatctt
    10681 gcgcgagcca ttgccgcgga cgatggtgaa agcgtccttg accggcaggg ccgcgaagtc
    10741 gatcgccagc agcttctcct ggcgctcctc ggtcaggttg cgcttctgcc tggtgtcgat
    10801 cagctggccg tggaagagga aattgccttc ggcgtcactg tagtacacat ccgtgccgac
    10861 ccgcacctcg aacaggccgg gcatgggggt cttgctgacc tcgtcgatct tgcccatctg
    10921 cggcagccgc tcgggcaggt tcttgcggat ggccgcttcc tgggcgtgcg cgaccccggc
    10981 aaacagcccg gatgcgagca gggccgccag cacggctttc ttgatcaaat gcattgttgt
    11041 tccattactt ccagaatcaa acccgcccca tcgcctgccg cgccacccat tgcttgagcg
    11101 gaccgctgcg atcgaacccc gtcatgcccc agttgcgcag catccccca
//
LOCUS       DASWIY010000489         8552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3312821, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000489 DASWIY010000000
VERSION     DASWIY010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3312821"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="cpaB"
                     /locus_tag="VGA59_17530"
     CDS             complement(<1..842)
                     /gene="cpaB"
                     /locus_tag="VGA59_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX9721521.1"
                     /translation="MKNMKAIVFLVLAVVMGLAAAVYAASWVAQRGNIASTQVVVAAV
                     DIELGSKINPQMLTTSSWPTESVPQDAFKDVTELQDRVVKASVLRGEAIVSRKLAPVG
                     TQGGLSAVIAEGKRAMTVRVNDVVGVAGFALPGNYVDVMLNTQQMDKVRGGNANQISM
                     TVLEHVLVLAVAQEAGRDETKPKVVSAVTLELTLEDAEKLDLARSVGSLSLVLRNQLD
                     KKTVATGGVTKSQLLGLKQVAVEESPAPAAAKPVQVVMRRVAPKPVTAVMSGPPPVCV
                     EVIR"
     gene            complement(871..1458)
                     /locus_tag="VGA59_17535"
     CDS             complement(871..1458)
                     /locus_tag="VGA59_17535"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX9721522.1"
                     /translation="MNDAASSLLELLGMLVTSPHTLALFALLVAAGVIDYRTMRIPNW
                     LTVGGILVGLLSSTLSTGRPLDGFLGASAGMAVGLAVLLPFYALRVMGAGDVKLMAAI
                     GAFLGFPAILYAVLFTFIVGGVAAVAFAVSHKVSRRMADNLGAIAMSMALAAMTRTSP
                     AASLKQGGSIGKLPYAISIGIGTTAFVVARQLGFA"
     gene            complement(1670..1954)
                     /locus_tag="VGA59_17540"
     CDS             complement(1670..1954)
                     /locus_tag="VGA59_17540"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9721523.1"
                     /translation="MFKEQRTEPRERLALPLRLRDGSHAVTRDISASGLYFEMEGDHQ
                     DVGGLVDFEMELPDLRMKFTAVGRIVRVEHRPGTTGVAVKLLSPRLEPME"
     gene            2257..2919
                     /locus_tag="VGA59_17545"
     CDS             2257..2919
                     /locus_tag="VGA59_17545"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9721524.1"
                     /translation="MRSIQVHLVAQPLVSWGLERLVQTAHPRLEVVGVAATTAESLPL
                     LERKSADVVVLDLDGEESSDSVAELQTRTRAKILVLTGSPDVVMLDRAVLAGVRGVVR
                     KREPPATLLKAIEKVHEGELWIDRGATSRIFMEMARQKAAEKNDPDKSKISTLTMRER
                     QTIMALASDAAAPGKVIAGRLCISEHTLRNHLTSIYSKLGLSNRLDLYAYASKHSLNK
                     PG"
     gene            3162..3989
                     /locus_tag="VGA59_17550"
     CDS             3162..3989
                     /locus_tag="VGA59_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX9721525.1"
                     /translation="MTLLAILIATLAAGIGSVWLAAGLMRLGLPDHSGDESVHGGPPH
                     LLSLAAGALLATSFMHLLPEAFESQAGAKELFSTLLVGLVFFFLLDKAELWHHGHEHH
                     RERGAGGNEHEHEHDHGHRHHSPPLPAGGWAVLTGDSVHCFGDGILIASAFAADLRLG
                     MVAALAVLAHEVPHHIGDLAVLRLSSPNRRAALVKVSLAGAVTPLGGLTGWWLVDQLQ
                     DYLPYFLAVASSSFVYVALADLIPQLQKRLSARDTAAQIAWLLAGLGLVTVVSGMAH"
     gene            4099..4989
                     /locus_tag="VGA59_17555"
     CDS             4099..4989
                     /locus_tag="VGA59_17555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEX9721526.1"
                     /translation="MAEERDLTHDPISGQGTHAADDARQAVERDTESASLSPGDHPAA
                     AYPASEADYWRESYSREPYYEGGRGFEDYGTAYETGWVGYSVYGGEFEAADRVMANDW
                     TVRKGVSTLSWDQARPAARAAWQRAENARSYVTNGSASRDDVIEALNDLLENARDGEL
                     GFREAAEHTRTPSLSALFGRRADTCRQAAAELQEQIQRLGGTVDEGGTVTGAAHRVWV
                     HIRGLFGGASDATMLDECERGEDAAVARYRKALKQNLPHDIHAMVQRQFEGAQRNHDM
                     IKALRDRARAEASTKTDDVS"
     gene            5284..6165
                     /locus_tag="VGA59_17560"
     CDS             5284..6165
                     /locus_tag="VGA59_17560"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX9721527.1"
                     /translation="MKCTSFCILTALSAFVLAGAARAESEAEYVNPGSASAVAPEQAK
                     PKPRPAVRAVVFAPAAAANAKRMSAQQREERRFLKEAAAGSRFEAEAARMALGKSSDP
                     GVRSLAATLINYHASANNELLHMLHGRGMAAPMLANDQRKVLNRLGKLHGARFDREFV
                     EQVALKHQQEDVQIYEKASLVVQEPRLRAWIIRTLPTLRYHLATAERIAPADIKLARP
                     AADAPAAAATGNRLVGRASAATRSMGAGPEPRGGGLQSGELQFGGTQSGGMVFGGAAQ
                     LGITRPIAARPSESSNR"
     gene            complement(6143..6652)
                     /locus_tag="VGA59_17565"
     CDS             complement(6143..6652)
                     /locus_tag="VGA59_17565"
                     /inference="COORDINATES: protein motif:HMM:NF024609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HEX9721528.1"
                     /translation="MKFSPSLLAALAAVLFTGTAGAQLKPPRSTAQPAAAPAATPAQT
                     PAPAAPDKAAAEKEAAGKLAAAGWLVLLDRRDWGRAWETSSSVFRGSVPLATWMDGIP
                     KVREPLGALVERTPAESAYKTTLAGRPDGEYVTVIFLSKFAKRELQEVVTVVRDTDGK
                     WRATGYSTR"
     gene            complement(6733..7629)
                     /locus_tag="VGA59_17570"
     CDS             complement(6733..7629)
                     /locus_tag="VGA59_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9721529.1"
                     /translation="MNISIGTASWTDKTLIACRRFYPPEARSAEARLRFYATQFPIVE
                     VDSSYYGMPTPSNAQLWADRTPDHFVFNVKAFRLFTGHQTSPIVLHKDIQQALGPNVP
                     ATLYYKDLPEEILGELWRRFSEALEPLRQAGKLGAVHFQFAPWMLRNREGMAHVRECV
                     ARMEDHLLSVEFRNKSWFDGDNMDKTVAFERELGVVHTIVDGPQGFANCVPCVWESTN
                     PALALVRLHGRNQETWNIKSSASSSRFNYWYSQEELAVLVPQIRRVAGLAGAVHVLFN
                     TNYEDQGQVNARLMTRLLRGKA"
     gene            7770..8231
                     /locus_tag="VGA59_17575"
     CDS             7770..8231
                     /locus_tag="VGA59_17575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein A"
                     /protein_id="HEX9721530.1"
                     /translation="MNDKKALNRMQLSDALAEFPKTEAAAAPRGGWLRAIRQALGMTQ
                     AQLGARVGIARQSVQDLEAAEANRRITLDSLDRLAQAMGCRVVYAVVPETGTLDDLRE
                     RRARTVATTLLKPTEHSMKLEAQGVSTSESERQRKLLVQTLLTGSPRKLWQ"
     gene            8239..>8552
                     /locus_tag="VGA59_17580"
     CDS             8239..>8552
                     /locus_tag="VGA59_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX9721531.1"
                     /translation="MNFDYPQGATPLDPDEAAELIPSHINTHKQLNEWELVNIVEGER
                     WAFGRKHRDLLDGAFESIHPFANGNGRLSRTMADLLLVHHGASRFSWGSGANLVEEGA
                     VR"
BASE COUNT         1564 a         2745 c         2794 g         1449 t
ORIGIN      
        1 ctgcgaatca cctcgacgca cacggggggc ggaccggaca tgactgcggt cactggcttc
       61 ggcgcgacgc ggcgcatgac cacttggacc ggcttggccg cggcgggggc aggcgactct
      121 tcgacggcga cctgcttgag cccaagcagc tggctcttcg tcacgccgcc ggtggccacg
      181 gtcttcttgt cgagctggtt gcgcaggacg agcgagaggc tgccgacgct gcgggcgagg
      241 tcgagcttct cggcgtcttc cagtgtcagc tccagcgtga ccgcgctgac cacctttggc
      301 ttggtctcgt cgcggccggc ttcctgggcc acggccagga ccaaaacatg ctccaggacg
      361 gtcatgctga tctggttcgc attgccgccc ctcaccttgt ccatctgctg agtgttcaac
      421 atcacgtcca cgtagttgcc cggcagtgcg aaacccgcta cgccgacgac gtcgttgacg
      481 cgaacggtca tcgcgcgctt gccttccgcg atcacggccg acagcccgcc ttgcgtaccc
      541 accggagcga gcttgcgact cacgatggcc tcgccgcgca aaacgctggc tttcaccacc
      601 cgatcctgga gctccgtaac atccttgaac gcgtcctgcg gcaccgactc ggtgggccaa
      661 ctggaggtcg tgagcatctg gggattgatc ttgctgccca gctcgatgtc gacggccgcg
      721 acaacgacct gggtcgacgc gatgttgccc ctctgggcta cccaactcgc cgcgtagacg
      781 gccgctgcca ggcccatgac cacggccagg actaggaaga cgattgcctt catgtttttc
      841 ataccggttc tccttgaatg gggctacgcg tcatgcgaag cccagttgcc ttgccaccac
      901 gaaggcagtg gttccaatac cgatgctgat tgcgtacgga agctttccaa tggatccgcc
      961 ctgtttgagc gaagccgccg gggatgtgcg tgtcattgct gccaaggcca tggacatggc
     1021 gatggcaccc aagttgtccg ccatgcgccg gctgacctta tgggagaccg cgaaggccac
     1081 cgccgcgacg ccgccaacga tgaaggtgaa cagcaccgca tacaggatgg cgggaaagcc
     1141 caggaaggca ccaattgcag ccatcagctt gacgtcgccg gcgcccatca cccgcaatgc
     1201 gtagaacggc agcagaacgg caaggccgac tgccatgccg gccgatgcgc ccaggaaacc
     1261 gtcgagcggc cttcccgtgc tcagggtgct ggacagcagg ccgacgagaa tgccgcccac
     1321 ggtcagccag ttgggaatac gcatggtccg gtaatcgatc acccccgcgg cgacaagcag
     1381 cgcaaacagg gcgagcgtgt ggggactcgt gaccagcatg cccagcagtt ccagtagcga
     1441 agaagcggcg tcgttcatgt cgaaatggtt caggtccgat gtaacggagt tatgcgaagc
     1501 aaatattcac cccgtggcgc agccgcacaa tggttgatcg ctcccgattt gcctaggaca
     1561 acactcccgg cgggtttgcc cgaatccgca caaacagcct gggtggtcag gcgcctgccg
     1621 gcgaccttgc ggtccagctg tttgtgttcg agggaatgtg cggaccgctt cattccatcg
     1681 gctccagacg gggcgacagc agcttcaccg cgacaccggt ggtgcccggg cggtgctcga
     1741 cgcgaacgat ccgacccacg gcggtgaatt tcatgcggag atcgggcagt tccatctcga
     1801 aatcgaccag gccacccacg tcttggtgat cgccctccat ttcgaagtag aggccgctgg
     1861 cgctgatatc cctcgtcacc gcatggcttc cgtcgcgcaa ccgcagtggc agtgccaggc
     1921 gctcgcgggg ttctgtgcgc tgctctttga acatgtgggc cagtgtgctt cgcggcccgg
     1981 atttacctct ggttggtttc gccctattta tctaggtgga ggtcattgca tggctgggtc
     2041 ttttccggga cgaaagtacc ggtccggcga actccggctg acgcgaaaaa ccctcataag
     2101 cgtctgcaaa atgcaccaat ttgtaggaca acttcccgtg tttcgataga tttgtaaaaa
     2161 atatcctaaa aaaaaagcac agcagggcac ggttcattac gataagaccc tacctgttac
     2221 ggaactgcgg gatttgctct tatgttgatc agccatatgc gttccatcca agtgcacctg
     2281 gtggcccagc cgttggtgtc gtggggactc gagcggctgg tgcagaccgc gcatccccgg
     2341 ctcgaggtgg tgggtgtggc ggccacgacg gccgaaagcc tgccgctgct ggaacgcaag
     2401 tcggcggatg tggtggttct ggacctggac ggcgaggaat ccagcgattc cgtggcggaa
     2461 ttgcaaaccc gcacccgcgc gaagatcctg gtgctcaccg ggtcgcccga tgtggtgatg
     2521 cttgaccggg ccgtcctcgc gggggtgcgc ggagtggtcc gcaagcgcga gccgcccgcc
     2581 accttgctca aggcgatcga aaaagtccac gagggcgagc tgtggatcga tcgcggcgcg
     2641 accagccgca tcttcatgga gatggcgcgc cagaaggccg cggagaaaaa cgatccggac
     2701 aagtccaaga tctcgacgct gaccatgcgc gagcgccaga ccatcatggc cctggcgagc
     2761 gacgctgcgg ccccaggcaa ggtgatcgcg gggcgcttgt gcatcagcga acacactttg
     2821 cgcaatcacc tcacctcgat ctacagcaag ctgggcctgt ccaaccgcct ggacctgtac
     2881 gcctacgcca gcaagcacag cctgaacaag ccggggtgaa agcccacccc cgaagcgcct
     2941 tcggcgcctc cccctcgagg gggcgccacc agcggcccgg caaagccggt tccgcggtgg
     3001 cccgcgccat gggaaactta gatctacctc gggtcgcctt tgattcgccg gcaccggcac
     3061 ccttcttgac accattgcta agataaacgc aagaaccggc cctcgccggc aacctcgaga
     3121 gggaaaaggg tcaagccgcc cagcgcgcct agcccctcat catgaccttg ttggcaatac
     3181 tgatcgcgac cttggccgcg ggcatcggaa gcgtttggct ggccgcgggc ctgatgcgcc
     3241 tgggcctgcc tgaccattcc ggcgacgagt ccgttcacgg ggggccgccg cacctgctta
     3301 gcctggcggc cggcgcgctg ctggccacgt cgttcatgca cctgttgccg gaggctttcg
     3361 aaagccaggc cggcgccaag gaactgtttt cgaccttgct ggtgggcctg gtgttctttt
     3421 tcctgctgga caaagccgag ctctggcacc acgggcacga gcaccatcgc gagcgcgggg
     3481 ctggcggcaa tgaacacgaa cacgaacacg atcacggtca tcgacaccat tccccgcctt
     3541 tgcccgcggg cggctgggcc gtgctcaccg gcgacagcgt ccactgcttc ggcgacggca
     3601 tcctgatcgc ctcggcgttc gccgccgacc tgcggctggg catggtcgcc gcattggccg
     3661 ttcttgcgca cgaggtgccg caccatatcg gtgacctggc tgtgttgcgc ctgagcagtc
     3721 ccaaccgccg cgccgcgctg gtcaaggtgt cgctggccgg cgcggtgacg ccgctgggcg
     3781 gcctgacggg ctggtggctg gtggaccagc tgcaggacta cctgccttac ttcctggccg
     3841 tggccagcag cagcttcgtc tatgtcgcgc tggcggacct gattccccag ctgcagaagc
     3901 gcctgtcggc ccgcgacacg gccgcgcaga tcgcctggct gctggcgggc ctggggctgg
     3961 tcaccgttgt cagcggcatg gcccattaac gcggctcagt cgccggcgtc ctacatgcgg
     4021 gagcggcgtg caccgaccgc gatcggggct cgcgcgagga ataaattgcg ccagcgcagt
     4081 ttgcgtaagg agcatgccat ggctgaagaa cgagacctga ctcacgaccc gatcagcggt
     4141 caaggcaccc atgccgcgga cgacgccaga caggctgtgg agcgggatac cgaatcggcg
     4201 agcttgagcc cgggcgacca tcccgccgcc gcctaccctg ccagcgaggc cgactactgg
     4261 cgcgagagct attcccgcga gccttattac gagggcggcc gcggcttcga ggactatggc
     4321 acggcctatg aaaccggctg ggtcggctac agcgtgtacg gcggcgagtt cgaagccgcg
     4381 gaccgcgtga tggccaacga ctggaccgtg cgcaagggcg tctcgacgct gtcatgggac
     4441 caggcccgtc ccgccgcgcg ggcagcttgg cagcgcgccg agaacgcgcg ctcctatgtg
     4501 acgaacggaa gcgcaagccg cgacgacgtg attgaagcgc tcaatgacct gctggaaaac
     4561 gcacgcgatg gagaactggg ctttcgcgaa gcggccgaac acacgagaac gccgagcctg
     4621 agcgccctgt tcgggcgtcg cgcggacacc tgccgacagg cagccgccga actgcaggag
     4681 cagatccagc gcctgggcgg cacggtggac gaaggcggca cggtcaccgg cgccgctcat
     4741 cgcgtgtggg tgcatatccg cggcctgttc ggcggtgcca gcgacgcaac catgctcgat
     4801 gaatgcgagc gtggcgagga cgcggccgtg gcgcgctatc gcaaggcgct caagcagaac
     4861 ctgccgcacg acatccatgc gatggtgcag cggcagttcg aaggcgcgca gcgcaaccac
     4921 gacatgatca aggcactgcg cgaccgggcc agggccgagg ccagcacgaa gaccgacgac
     4981 gtctcctgaa ccgggtgacc ggctccggcc ggtcatgcat cgtcagcggt aatacggatt
     5041 gttcgggcgt gccggccgcg cctcttgccg ccatggattg cgggtcaagc ccgcaatgac
     5101 agggaagatg gctgccggcc ggaggcaaga gtggcgccaa caagacgggc agctgacggg
     5161 atcgagcggc ggcggcttgt cctacgccgc catcaggcgt catccgacaa gccggcgtgc
     5221 cgccactgcc caacattgag ccattgcctg ccggcagttc gagcaggtcc ccggggaaat
     5281 gtcatgaagt gcaccagctt ttgcattttg accgcgctgt ccgctttcgt tctggcgggt
     5341 gcggctcgcg cggaaagcga ggcggagtat gtcaatcccg gctcggcgtc ggcggtcgcg
     5401 ccggagcagg caaaacccaa accccggccg gcggtgcgcg cggtcgtgtt cgcgccggcc
     5461 gccgcggcca atgcgaagcg catgtccgcg cagcagcgcg aagagcgccg cttcctgaag
     5521 gaggcggccg ccggcagccg cttcgaagcc gaagccgcgc gcatggccct gggcaagtcg
     5581 agcgatccgg gcgtgcgctc gttggcggcc acgctgatca attaccacgc gtccgccaat
     5641 aacgaactgc tgcacatgtt gcacgggcgc ggcatggccg cgccgatgct cgccaatgac
     5701 cagcgcaagg tactcaatcg cctggggaag ctgcatggcg cccggttcga tcgtgaattc
     5761 gtggagcagg tggccctgaa acaccagcag gaagacgtcc agatctacga aaaggccagc
     5821 ctcgtcgtgc aagagccgcg gctccgggcc tggatcattc gaacgctgcc gacactgcgc
     5881 tatcacctgg cgacggcgga gcggatcgcg cccgccgaca tcaagctggc caggccggcg
     5941 gcggacgcgc cggcagcagc ggcgacaggc aaccgattgg tcggccgcgc cagtgcggcc
     6001 acgcggtcga tgggcgccgg gccggaaccg cgcggtggcg gcctgcagtc cggcgagttg
     6061 cagttcggcg gaacgcaatc cggcgggatg gtgttcggag gagccgccca gctgggcatc
     6121 acacggccca tcgcggcccg gcctagcgag tcgagtaacc ggtagcgcgc cacttgccgt
     6181 cggtgtcgcg cacgaccgtg acgacctcct gcagctcgcg cttggcgaac ttgctcagga
     6241 agatgacggt cacgtattcg ccgtccggcc gccccgccag ggttgtcttg taagccgatt
     6301 cggcgggcgt gcgttcgacc agggcgccca gtggctcgcg caccttgggg atgccgtcca
     6361 tccaggtggc cagcggcacc gagccgcgga acacgctgct ggatgtctcc caggcgcggc
     6421 cccagtcgcg ccggtccagc aggaccagcc agcccgcggc ggccaacttt ccggctgctt
     6481 ccttttctgc ggcggctttg tcgggggcgg caggggcggg ggtttgcgcc ggcgtcgcgg
     6541 cgggagcggc agcgggttgc gccgtactgc gcggcggctt caattgagcg ccggccgtgc
     6601 cagtgaacaa tacggcggcc agggccgcca gcagagatgg ggaaaacttc attcgcgctc
     6661 cgcacatgga aaaggatgac gaggagtcta gcccgggtga gttcctcacc cggcgaccgc
     6721 acatcctttt acttacgctt taccgcgcag caatcgggtc atcagccgcg cattgacctg
     6781 gccctgatct tcgtaattgg tgttgaacag cacgtgcacg gcgccggcca ggccggcgac
     6841 gcggcggatc tgcggcacca gcaccgccag ctcctcttgc gaataccagt aattgaagcg
     6901 gctggacgag gcggaactct tgatgttcca tgtctcctgg ttgcggccat gcagccgaac
     6961 cagcgccagt gcggggttcg tcgactccca gacgcacggg acgcaattgg cgaagccttg
     7021 cggcccgtcg acgatcgtgt gcaccacgcc cagctcgcgc tcgaaggcca cggtcttgtc
     7081 catgttgtcg ccgtcgaacc agctcttgtt gcggaattcc acggacagga ggtggtcctc
     7141 catgcgcgcc acgcattcgc gcacatgggc catgccctcg cgattgcgca gcatccacgg
     7201 cgcgaactgg aaatgcaccg ctccgagctt gccggcctgg cgcagtggct ccagcgcctc
     7261 gctgaagcgg cgccacagct cgcccagaat ctcctcgggc aggtccttgt aatacagcgt
     7321 cgccggcaca ttcggcccga gtgcctgctg gatgtccttg tgcaggacga tgggcgaagt
     7381 ctggtggccg gtgaacaggc gaaacgcctt gacgttgaac acgaaatggt cgggcgtgcg
     7441 atcggcccag agctgcgcgt tcgacggcgt gggcatgccg tagtagctgg agtcgacctc
     7501 gacgatcgga aattgcgtcg cgtagaagcg cagccgcgcc tcggcgctcc tggcctccgg
     7561 gggatagaag cgccggcacg cgatcagcgt cttgtcggtc caggaggcgg tgccgatgga
     7621 gatgttcatg ttcaaactgg atcgtctgta ttaacataca gtacaacaac cgagtaaaag
     7681 cagggtttag ccttcttttc ttgttgacga agctggtttt aaccagcata ctacaaaaat
     7741 aaagcaggct atatcctgca tatgcatcta tgaacgacaa aaaagccctc aacagaatgc
     7801 aactgagcga cgcgctagct gagtttccca agactgaagc ggctgcggcg cctcgcggtg
     7861 ggtggcttcg cgcgatccgg caggccttgg gcatgacgca ggcgcagctc ggcgcacgtg
     7921 ttggcatcgc acgccagtcg gtccaggacc ttgaggccgc cgaggcaaac aggcgcatca
     7981 cactcgacag ccttgaccgc ttggcccaag caatggggtg ccgggtcgtt tacgcggtgg
     8041 tgccggaaac gggcacgctg gacgacctac gcgagcgtcg ggcccggacg gtggccacga
     8101 cgctgctcaa accaacagag cattcgatga agctcgaagc acaaggcgta tccacgtcgg
     8161 aaagcgaacg ccagcgcaag ctgctggtgc agactttgct caccggcagt ccgcgcaagc
     8221 tctggcagtg agcgcggcat gaacttcgat tatccgcagg gcgcgacccc gttagaccct
     8281 gacgaagcgg cggagctaat cccttcgcac atcaacacgc acaaacagct caatgaatgg
     8341 gaactggtca atattgtgga aggtgagcgc tgggctttcg ggcgcaagca tcgtgactta
     8401 ttggacggtg cattcgaatc catccatccc tttgccaacg gcaacggccg tctttcccga
     8461 accatggccg atctgctgct tgtgcatcac ggcgcgtccc ggttcagttg gggctcgggc
     8521 gccaatctgg tggaagaggg cgcagttcgg ca
//
LOCUS       DASWIY010000490        31033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_718159, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000490 DASWIY010000000
VERSION     DASWIY010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 31033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31033
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718159"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..238)
                     /gene="ccoS"
                     /locus_tag="VGA59_17585"
     CDS             complement(101..238)
                     /gene="ccoS"
                     /locus_tag="VGA59_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEX9721532.1"
                     /translation="MDILFILIPLSVALALLILGALAWAVWRGQFEGVDAEAERILQS
                     D"
     gene            complement(270..2618)
                     /locus_tag="VGA59_17590"
     CDS             complement(270..2618)
                     /locus_tag="VGA59_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX9721533.1"
                     /translation="MPTAVLTIDARAAGAAPASGWAVLDEPAEWEGFSRAVAGREGCW
                     ESFLAIEGMHCAACSLTVEAALAQLPGVDSVRVNGASATARLVWSPQSSRPSAWLAAL
                     KRAGYGAVPAGDLIAAAPRRQAQRLLLWRWLVAGFCMMQVMMYAFPAYTAAPGEITSD
                     IEALLRWASWVLTLPVVLFSCWPFFTAAARDLRNGRIGMDVPVALGIAIAFGASTAAT
                     FDPGGSLGREVWYDSVTMFVFFLLSGRLLEQRLRDRTAGSLEALMRRLPQTIERQRFE
                     DGAFERVPVRRLAAGDLVRVLPGEVFPADGCVVDGETRVDEALLTGESRPLLRRKGEP
                     VVAGSHNLTGVVLVRVERTGSQTRYAGIVALMEQASMDKPRLARIADRIARPFLVGVI
                     LAAAAAAAWWWPTDPGSALGVAVAVLIVTCPCALSLATPAATLAAAGALARAGVLVRR
                     LQALEACASVDTVVFDKTGTLTEDRMALVATHARPGTEPAEALALAAALARHSLHPAS
                     RAIAGAAGAVGWAATHVAEVPGSGVMGLVAGEGGQPRELRLGSASFCDAPARSRDAAG
                     LESHLADDLGWLASFELDEAMRGDAPAAVNALRELGLEVQLLSGDRAAAVKRLARRAG
                     VERAFGGCSPEDKLDHVRLAQQRGQSVAMVGDGVNDGPVLARADVSIAMGQAVPLAQA
                     KSDFVVLGGQLAAVASLMRHARRTRAIVRQNLAWAAVYNAVSVPLAVAGAMPPWLAGL
                     GMAASSLLVVANSARLARMEPERCIESSSLSAQVLPGSTANA"
     gene            2724..3167
                     /locus_tag="VGA59_17595"
     CDS             2724..3167
                     /locus_tag="VGA59_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9721534.1"
                     /translation="MYQKLLVATDGSTLSKKAVRSAVELAGAVGAELVALYVVPRYPV
                     SYFEGGITVSTQDIARTEKQWADEGQAVVDAVQQAAQAKGVKAKGIIAKSDLVAESIM
                     ATAKKQKCDLIVMASHGRKGFKRILLGSETQHVLTHSTVPVLVLR"
     gene            3190..3612
                     /locus_tag="VGA59_17600"
     CDS             3190..3612
                     /locus_tag="VGA59_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9721535.1"
                     /translation="MKILLAADGSEYTKKAAAFLVTHERLAGPDDELIVLHVQSAVPP
                     RVKAMLGASAIRAYHSEEAAKVLSPIERFLKRHPLNFRTLWVVGSPAIEVVRVAKQEG
                     VHMIVMGTHGHGMLGRALMGSVAQNVVSECDIPVLLVK"
     gene            3649..5550
                     /locus_tag="VGA59_17605"
     CDS             3649..5550
                     /locus_tag="VGA59_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HEX9721536.1"
                     /translation="MRGPWTLTAKLYTSGISFLVLALASIGLTLWVTWNLEGGAAAVN
                     EAGRMRMQTYRLALEVSGTADPAVVLSLARSFDESLALLRAGDPSRPLFVPWSRASRE
                     YFDKVNESWGELRGRWLDSRPTPAQAAREAGAFVALVDGLVAAIESQIGRWTAILHMF
                     QLAMMGLAIGSAVMMLYAGYLFVLNPLARLQRGLTAVQQGDLSTRVEVESADEFGQLS
                     AGFNDMARTLQSLYGSLEEKVREKTAKLEVERRRLADLYEVSAFMAHATGLDELAHGF
                     ARQVRAVAQADAAAVRWSDEGNQRYVLLAADNLPQNLAQAENCLATGTCHCGQAHADA
                     AIRVIPIRAARPAPMDHCLRSGFRTLVSVPVKLHERMLGEVDLFFRKEVRLDPEDRDL
                     LDTLASHLASAMESLRASALEREAAVAQERSLLAQELHDSIAQSLAFLKIQAQLLRDA
                     LRRNDTASMGRVIGELDAGVRESYADVRELLVHFRTRTSKEDIEPALRTTLSKFEHQT
                     GLPARLEMEGHGVALAPDVQVQVLHIIQEALSNVRKHSQAHEVALRVSSDPCWRFEVH
                     DDGVGFAADSASSNETRVGLRIMQERAERIGATVQVASAPGTGCSVVLELPRARAEAA
                     AASPIGAAA"
     gene            5547..6248
                     /locus_tag="VGA59_17610"
     CDS             5547..6248
                     /locus_tag="VGA59_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9721537.1"
                     /translation="MNGTGKAAVRLMVVDDHTLFRRGLVALLASDSSLDVVGEASDAG
                     EAVHKAATLRPDVILLDNHLPGATGIQALPGLKEAAPAARIILLTVSEDEQDLQAALR
                     AGAHGYLLKTIEGDLLAQSIHRAMRGEPVISSEMTGKLVTAFQSSASPSPPVPSPPDP
                     LAQLSAREQQVLQQIALGASNKEIARTLSIAETTVKIHVQHILRKLDLTSRVQAAVFA
                     AGGSQRPQGAGSDDS"
     gene            6441..7790
                     /locus_tag="VGA59_17615"
     CDS             6441..7790
                     /locus_tag="VGA59_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEX9721538.1"
                     /translation="MHRKHQPPPADAIVTRESWSVLAVSTLAFTVCFMVWMMFGVIGI
                     PIKKTLNLNSTEFGLLTAMPVLTGSLIRVPLGIWTDRFGGRIVMAVLMAITVPAIWFM
                     SYATQYWHYLVIGLFVGLAGGSFSVGTPYVARWFPRKRQGLAMGIYGAGNSGAAVNKF
                     IAPALVVAFGWTMVPQVYAAVMLGTLLLFWFFSYSDPKHLVSSEVKFMDQLRSLKDPK
                     VLRYCQYYSIVFGGYVALSLWMVQYYVGEFGLDIRWAALLAAAFSLPGGVLRAIGGWL
                     SDKFGAHSVTWWVMWVSWICLFMLSYPQFEVTFLTVNGPKTFALGLNVYAFTALMFIL
                     GVAWAFGKASVFKYISDDYPHNIGTISGIVGLAGGLGGFVLPIMFGALMDITGVRSSA
                     FMLMYGVVWISLIWMYFTEVRRTEVMGDHAPESPSLHLPHGAASPTSRVSAAMPARA"
     gene            7815..9197
                     /locus_tag="VGA59_17620"
     CDS             7815..9197
                     /locus_tag="VGA59_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015514 - nitrite efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015707 - nitrite transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HEX9721539.1"
                     /translation="MSTALIAHWNPEDKGFWRTQGAPVAQRNLWISIPALTLAFAVWM
                     VWSVVVVNLPTVGFKFSTNQLFWLAALPALCGATLRIFYSFAVPIFGGRRWTAISTAS
                     LLLPAAGIGFAVQDPATSYEVMVLLALLCGFGGGNFASSMANISFFFPKDRKGYALGM
                     NAGLGNLGVSVVQFVVPLVITAGVFGTIGGDPQAITKAGVQTSMWLQNAGFIWVPFII
                     VSALAAWFGMNDLADAKASFADQAVIFKRKHNWLMCWLYLGTFGSFIGYSAGFPLLIK
                     SQFPGINALQYAWLGPLVGALIRPVGGWLSDKVGGARVTFWNFIAMALGVLAVLQFLP
                     ANGQGGNFTGFLISFMLLFATSGIGNGSTFRMIPVIFMTERQRAAAKNAPAQEQAVKD
                     ANKEAAAVLGFSSAIGAYGGFFIPKSYGTSITMTGGPEAALYTFIVFYVTCVAITWWY
                     YSRKNAPMPC"
     gene            9424..13266
                     /locus_tag="VGA59_17625"
     CDS             9424..13266
                     /locus_tag="VGA59_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEX9721540.1"
                     /translation="MSHFLDRLTYFSQPREPFSGDHGQTTEEDRTWEDAYRARWAHDK
                     IVRSTHGVNCTGSCSWKIYVKGGIVTWETQQTDYPRTRWDMPNHEPRGCARGASYSWY
                     LYSANRVKYPMVRGRLLKAWREARRSMPPVQAWAAIVADDAKRKGWQSVRGLGGFTRS
                     SWDEVNEIIAAANIHTIRQHGPDRIIGFSPIPAMSMVSYAAGSRYLSLIGGVCMSFYD
                     WYCDLPPASPQIWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGTK
                     TVAITPDYSEVAKLSDLWMHPKQGTDAAVAMAMGHVILKEFYFDYGRRAAPSPDTPPG
                     GADAVRRPGGRNTSAYFDDYARRYTDLPMLVMLKEHTLPSGETVMVPDRYVRASDFNG
                     KLGQSNNPEWKTVAIADDGKVVLPKGAIGFRWGPDGRPDQGQWNLEDKEARHDQQVKL
                     KLSLLEGEAPSTETAKIGFPYFGGIETAHFQHNTQTDVLVRTVPVQRIALGKAGEERS
                     ALVATVFDLQAANYGVSRGLPGELAAESFDDDTPYTPAWQEKITGVPRDQIITVARQF
                     ADNADKTHGKSMVIIGAAMNHWYHSDMNYRGVINMLMMCGCIGQSGGGWAHYVGQEKL
                     RPQTGWTALAFALDWIRPPRQMNSTSFFYAHTDQWRYEKLGMEEVLSPLADKKAFAGS
                     MIDYNVRAERMGWLPSAPQLQTNPMQVVRDAQAAGMDGKEYTVKALKDGTLKMSCEDP
                     DHPANWPRNLFVWRSNILGSSGKGHEYFLKHLLGTSHGVQGKDLGADEAKPTEVVWHD
                     KAPEGKLDLVVTLDFRMSTTCLYSDIVLPTATWYEKNDLNTSDMHPFIHPLSTAVDPA
                     WQSRSDWEIYKGFAKKFSELCPGHLGVEKEVVLSPLMHDSPAELAQPFGVSDWKRGEC
                     ELIPGKTAPQITVVERDYPNVYKRFTALGPLMNKVGNGGKGIAWNTQTEVEQLGQLNG
                     LVTDAGQTQGMPNIDTDIDACEVVLQLAPETNGHVAVKAWEALSKQTGREHAHLALHR
                     EDEKIRFRDIQAQPRKIISSPTWSGLESEKVSYNAGYTNVHELIPWRTLTGRQQFYLD
                     HPWMSAFGEAFASYRPPVDLKTTAGIAGIKPNGNKEIQLNFITPHQKWGIHSTYSDNL
                     IMLTLNRGGPVIWLSEDDAKSAGIVDNDWVELFNINGAIAARAVVSQRVNPGMVLMYH
                     AQEKTINTPGSEITGMRGGIHNSVTRIVLKPTHMIGGYAQFSYGFNYYGTIGTNRDEF
                     VVVRKMNKVDWLDEEVAA"
     gene            13391..14926
                     /gene="narH"
                     /locus_tag="VGA59_17630"
     CDS             13391..14926
                     /gene="narH"
                     /locus_tag="VGA59_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEX9721541.1"
                     /translation="MKVRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRPGMEYAWFNNV
                     ETKPGIGYPKEWENQGKWNGGWKRNADGSIEPRQGNKWQLLMRIFSNPNLPQIDDYYE
                     PFTFDYDHLQSAPEMKAAPTARPRSLITGQRMEKIEWGPNWEEILGGEFAKRGKDANL
                     ANFDKVQKDMVGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDK
                     CRGWRMCVSGCPYKKIYYNWQSGKAEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVVL
                     YDADRIREAASVENEKDLYQAQLDIFLDPNDPKVIEQARKDGIPEAWLEGARKSPVWK
                     MAMDWKVALPLHPEYRTLPMVWYVPPLSPITSAANAGHLGANGEIPDVQQMRIPVQYL
                     ANLLTAGDTGPVIRSLERMLAMRAFQRGKHVDRVENRAVLDQVGLTVGQVEDMYQVMA
                     IANYEDRFVIPSTHREYAENTFDMKGGCGFSFGNGCSDGSTETSLFGGKKKRTIPIQV
                     EKV"
     gene            14923..15705
                     /gene="narJ"
                     /locus_tag="VGA59_17635"
     CDS             14923..15705
                     /gene="narJ"
                     /locus_tag="VGA59_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEX9721542.1"
                     /translation="MSDNNNPKLVMPDLIRHPATGLRQAQSWIAGQARDDKLVRHTLR
                     SLALLLGYPDEKLRGLLPALADAIDAEKALPSSRRIELRAFIGEMERADPIELEARYV
                     ELFDRGRATSLHLFEHVHGDSRDRGPAMIDLTQTYEKAGLLLGPHELPDHLCVVLEFA
                     STQPPALAREFLAEMGHILIAIFSALRKRESRYATVLAAVLELAGQKVEAVPVVDDEP
                     IDHAWAEPVVFDGCSTRGQAVPGQPQPIHIVRKTPPVEGARA"
     gene            15702..16481
                     /gene="narI"
                     /locus_tag="VGA59_17640"
     CDS             15702..16481
                     /gene="narI"
                     /locus_tag="VGA59_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEX9721543.1"
                     /translation="MNYLNQFLFGYYPYICLVVFLAGSLIRFDRDQYTWKSDSSQLLR
                     HGQLRWGSNLFHIGILFLLAGHGAGLLTPHFMYENFVSAGDKQLTAMISGGLFGLLGF
                     IGVTILLHRRLTDPRIRVNSKTSDIMMLVLLWVQFALGLATIPLSAQHLDGSLMIKLG
                     EWAQRIVTFRPGAAEMLVDAGWVFKAHMFLGMTLFLVFPFTRLVHVWSGFATLGYLVR
                     PYQVVRTRRKIAPSRPVAVAPAASTTQPVAGGMLEPGKATS"
     gene            16478..17341
                     /locus_tag="VGA59_17645"
     CDS             16478..17341
                     /locus_tag="VGA59_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9721544.1"
                     /translation="MSEMTGCGGGACTCGGGAHPVNGRALTAEPPAPPARINGIALHP
                     AGLRPDEAGLRELAWAELLRQEAVRQGLLPRQPGFEAPQLLPGDRQVIQAMLERAVQV
                     PQPSEQECRRYYEARQDHYVEGRMVNLRHILFAVTSGVDVHALAVRAETALLELSRQG
                     VDPGRFAALARELSNCPSGAEGGDLGWIAPQDCADELANELFHQKNPLRGIGLRPRLV
                     HTRYGFHIVEVLGRKQGRLLSFDHVRERIAVQLAQQSRAKALHQYIQLLAGQASVEGV
                     LLEATDSLLVQ"
     gene            17520..18734
                     /locus_tag="VGA59_17650"
     CDS             17520..18734
                     /locus_tag="VGA59_17650"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX9721545.1"
                     /translation="MHALCSDSGLRPWQFAGKSLLPVVQGGMGVGISAGGLAGAVAAL
                     GAVGTISSVDLRRHHPDLMALTENLDQEPDARERIDAANLEALGREIARARKLSGGRG
                     LIAVNIMRAVSAYEAYVRHSLACGVDAVVVGAGLPLDLPELARDYPKAALIPILSDAR
                     GVQLVVRKWEKKGRLPDAIVIEHPRLAGGHLGAAKVADLNDRRFDFDVVLPEVLAFLR
                     ASGLEGRIPLVAAGGIGSLEDIRRLQDLGATAVQLGTAFAVTTECDAHPEFKRILAEA
                     GPEDTVEFTSVAGLPARAVRTPWLDKYLRIESRLKGNAKVKPCTMRFDCLAHCGLRDG
                     NAAWGQFCIDKMLGHALAGQTGLGLFFRGAGRLPFGSQIRSVHDLMQWLMGGIRPAEL
                     LENCALETVHEA"
     gene            18724..22254
                     /locus_tag="VGA59_17655"
     CDS             18724..22254
                     /locus_tag="VGA59_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEX9721546.1"
                     /translation="MKRDEAALVPLAVQPISRDVLAEKYLKPGEKDLEDLFARVARAL
                     ASVETESQRDHWEALFHANMRAGAIGAGRIMSAAGTTLEATLINCFVQPVGDCIQGMD
                     ADGYPGIYEALREAAETMRRGGGVGYDFSRIRPRGAEVKATASIASGPCSYIDVFDHS
                     CATVESAGARRGAQMGVLRIDHPDVLEFITAKRKPGRWSNFNVSVAVTHAFMEALDAG
                     AAWSLVHRARPGAALLEKGAQQREDGMWVYRTLPAAELWDTVMRSAYDFAEPGILFPD
                     TINRDNNLRYCENIAATNPCVTADTWVLTTDGPRQVVDLIGRSFDAVVDGKACRVESQ
                     GFFKTGHKPVLSLCTREGHCLRLTADHPVRRVARKSRYTLQAEWTPAAQLRPGDEIIL
                     HDHRALGGWDGRGTEAEGYLLGLLVGDGTLKSDKAVICVWSPELRQVAQGAPAYAATG
                     ASGIMQAAEAAAATLPHRADFRGFQRPVAGRGEARMASAAVWHLAHDMGMRPGFKTVT
                     PDMEKSSSAFCVGVMRGLFDADGSVQGSTEIGVSVRLAQSDLPLLQAAQRMLLRLGVA
                     STIYTNRRPALARLMPDGSGGSRTYDTRAQHELVISGDNLRAYAERIGFADTDKAARL
                     EAVLGSYSRALNRERFTATVESLTEGAHEDVYDVTVADVHAFDANGIFVHNCGEQPLP
                     PYGCCDLGPVILTRFVHQPFGFGGTPEFDFKAFAASVALQVRALDNVLDLTFWPLPQQ
                     RAEAMAKRRIGVGFTGMGDALSMLCLRYDEPAGRDMAVRIARCMRDAAYGASVALARE
                     KGAFPTFDAQRYLESGTFASRLDEPLKKEIREHGIRNSHLLSIAPTGTVSLAFADNAS
                     NGIEPAFSWTYTRRKREGDGSVTEYDVEDHAWRLYRTLGGEMAKLPPYFVSAMEMPAA
                     AHVAMMEAVQPFVDTAISKTVNVSEDYPYEDFKGLYHEAWKAGLKGLATYRPNSIVGA
                     VLQAEATKPAAEAQPAPMDLMRTVIASRPKGALPAMAEKIEYWTQQGRQHLYLVVSFL
                     PLPDGRQRAIEFFMPVGQNGESQQWVTASMRLLSLAARGGFLDRALADMRKVAWDRGP
                     VRLGTYRKADGTHVPLWHDSEVAAIAYALQNLIAGRNGHENEAPAAVISAPAPAPMTG
                     AKCPECGAYAMIRKDGCDYCTQCGHLGACG"
     gene            22447..23088
                     /locus_tag="VGA59_17660"
     CDS             22447..23088
                     /locus_tag="VGA59_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX9721547.1"
                     /translation="MAPGKEDARVPTPFMGEPGSAQWAALLIQHRQTILPKRLAEPGP
                     DAAQLELIFGAAAAAPDHGELVPWRFVIIPAEARERLADVFAASLLERDSSATAEEVA
                     KAREKAYRSPLLLLAVVDVGPPADEVSPAERMISAGCAIQNMLLMATTMGFGSALTSG
                     KALQSQGLREMFSLRECEQAVCFVSIGTTLRRKPPRLRPEPSRYVSVLAPPAA"
     gene            23085..23633
                     /locus_tag="VGA59_17665"
     CDS             23085..23633
                     /locus_tag="VGA59_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9721548.1"
                     /translation="MSQPIRITSLVSGPAAGFEQPFEMLEACHERVQRMLALLQRLRE
                     HLPGHGADADARQAARDVMRYFDQAAPQHHRDEELHVFPPLLAQGDVATQDVVRRLQQ
                     DHLQMEPRWAAARAVLDAVAGGELAALLSAHEAALDAFAGPYADHIAAEEQHVYPAAA
                     ALLDEKAVAAMGAEMMRRRGVA"
     gene            complement(23642..24922)
                     /locus_tag="VGA59_17670"
     CDS             complement(23642..24922)
                     /locus_tag="VGA59_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9721549.1"
                     /translation="MNFEPSPTAIARLRQLDAFIERFVLPHNGAWHRCARQGEYPPFL
                     EDLKALARAEGLWNMCLPGLREDEPGTRLDNLDYAPLAEAMGRLPWSAEVFNCSAPDS
                     GNMDLLQRHASPDQRKRWLLPLLEGEIRSAFAMSEPDVASSDPTNLQTAVRREGGQLV
                     INGRKWFISGVAHPACRLLIVMCRNDGEPETASRHAGHSMVLVPIDTPGVNVIRNIPI
                     MQHTSPEGHCEIVLRDVRVPDENLLGGWGEGFAMAQGRLGPGRVHHCMRTIGQCELAL
                     ELACDRALERHAFGKYLADQSNVQDWIAESRLEIDQARLLVLRAAWGLDQAQRPQRLR
                     ADVAAIKVVAARLQTRVVDRAMQIFGAMGLSPDTPLAWLWTWGRAMRFLDGPDEVHLR
                     TVARQELARARERGGASSAYFTTPEQMEATPRIR"
     gene            complement(25057..27228)
                     /locus_tag="VGA59_17675"
     CDS             complement(25057..27228)
                     /locus_tag="VGA59_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX9721550.1"
                     /translation="MPQKIQIGDKWYVAATSASAEEQPQVLKNDQTFAMFDRFGDIQV
                     LAPGKEGLYHNDTRFLSHQEFQIDGARPLHLGSSVEESNSLLVIDLMNPDLPARSGRE
                     PLAKGNLHIFRAKLLWQGTCHEHIRVTHHGIERTSVRLEFTFDADFVDLFEVRGMKRE
                     RRGDRLQPEVMAHEVVLPYLGRDKHLRRTRIHFEPAPSSIDAGRAVFELALDPGTEHH
                     LYCTVLCEVDNAPPPTRISYEAALREANEARRAWLGGHCTVVTSNPLVNLWLDRSVSD
                     LSMLTTMLPTGPYPYAGVPWYSTTFGRDGIITALEYLWVDPSLARGVLTYLAANQATE
                     LDPQNDAEPGKILHEARHSEMALTREIPFGRYYGSVDSTPLFLVLAAAYYKRTGDAAL
                     VRSLWPNIRMALQWVDQWGDADADGFVEYSRRSDEGLVQQGWKDSGDSIFHQDGRLAG
                     PPIALCEVQGYVYEGKLAAAELARLMDEPQLAQALESQARQLRQRFHAAFWCEEIGSY
                     AIALDGNKQQCKVASSNAGHTLWSGIASPEHAPRIVDGLMGHRFFSGWGIRTIPAGAP
                     RYNPMSYHNGSIWPHDNALIAAGMARYGRTESAMQLLSSTFDASLHFDAHRLPELFCG
                     FPRRPGAGPTLYPVACSPQAWAAAAVFGMLQACLGLDIDVGKSEITLRTPRLPPFIDW
                     IRITRLGAPGPSADLLLQRYERNVGIEVVRKDANMLVTVVA"
     gene            complement(27255..28292)
                     /locus_tag="VGA59_17680"
     CDS             complement(27255..28292)
                     /locus_tag="VGA59_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9721551.1"
                     /translation="MKIAQVAPLYESVPPRAYGGTERVVSYLTETLVEMGHEVTLFAS
                     GDSVTSARLVPVVPWGLRLDPHKPDPLVWHTIMMDMVLKAAPTFDIIHFHIDILQLAL
                     VGQCETACLSTPHGRLDLPDIKPLFRRFGNHPMVSVSNSQRTPVPWANWRATVHHGLP
                     LGLYSLRARPQDYFAFVGRISPEKRADRAIEIARACGTPLRIAAKVDPADRDYFESVI
                     EPLLDDPLVTFVGEIGEKAKNDFIGNARALLMPIDWPEPFGLVMIEAMACGTPVIAYR
                     HGSVPEIIDHGVTGFIVSNQEQAIAAAKDIDRIDRGRCRQVFEQRFAARVMARHYLDV
                     YRQLVDARIPA"
     gene            complement(28461..29999)
                     /locus_tag="VGA59_17685"
     CDS             complement(28461..29999)
                     /locus_tag="VGA59_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HEX9721552.1"
                     /translation="MNNQNVFAALRAAFPADLDTQAVETDHGLVYSWRDLDRATAMIA
                     NLLLALKLPEGARIAAQVEKSVEAMMLYLATLRAGYVFLPLNTAYRSAEIEYFLGNAE
                     PAVVVCGALNFGWVSKIAFKTDSRHVFTLNEDRTGTLLDRAAHCGDRHEIAAKGEDDV
                     AAIVYTSGTTGRSKGAMLTHGNLLSNAKVLKDYWGWRSPEKGGDVLIHALPIFHVHGL
                     FVAIHGALLNGSKMIWLGKFDPALVIRKMSEATMFMGVPTLYVRMLAERGLDPAAARH
                     MRLFISGSAPLLLETFNEWKERTGHTILERYGMSETIMLTSNPFDAGDGERRGGTVGF
                     PLPGVGLRVRGDDGRELAAGEIGNIEVTGPNVFKGYWRMPGKTKEEFTEDGWFRTGDV
                     GKVDERGYVTIVGRSKDLIISGGYNVYPAEIESYINEMPGVAESALVGVPHPDFGEVG
                     VAVVIPKPGVQLDAEHIVAKLKLQLASFKIPKRCFVVDELPRNSMGKVQKNLLREQHR
                     QLFT"
     gene            complement(30002..30793)
                     /locus_tag="VGA59_17690"
     CDS             complement(30002..30793)
                     /locus_tag="VGA59_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9721553.1"
                     /translation="MPGQVLFTSEGTIAVVTLSHPGKLNAMSRAMWRELRAVFERIQS
                     DSQWRCVVVQGEGGTFCAGGDISEYPGFRFSEAALRDFHESDVWGGLAAMLACDVPLV
                     ARIEGACMGAGLEIASCCDIRVAAESAAFGAPIAKLGFPMAPREAQLVLRAAGESTVR
                     EMLLEAAVLPAVEMKNRGFLNRVAADAEVGAQAWASAQRVATLAPQAARLNKQALRAL
                     APRPWPAEASDQAGTQPNAGAYRYADSAEHREGIGAFLEKRKPGF"
     gene            complement(30796..>31033)
                     /locus_tag="VGA59_17695"
     CDS             complement(30796..>31033)
                     /locus_tag="VGA59_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721554.1"
                     /translation="NPDFWGDRRPVLLRFRQRQAYCRLPAGWCGDCLEFIHDKETKTV
                     NHSLSIRDVLRAAATGAGMLTALARAQPTWPARP"
BASE COUNT         5264 a        10648 c        10215 g         4906 t
ORIGIN      
        1 gttgtagagc gccgtttcgg agcgaatatc gttcattggg agaccttgtt gcgaaaccct
       61 tcaagtttcc ccgcgaggcc gcccatggaa ttgacgaaaa tcaatccgac tggagaatcc
      121 gctccgcctc cgcatccacg ccctcgaact ggccgcgcca gacggcccag gccaatgcac
      181 caagaatgag cagcgccagc gcgaccgaca gcgggatgag gatgaagagg atgtccatga
      241 attaaccgtt cagtgttcaa tgttcagagt taagcgttag cggtggaccc gggaagtact
      301 tgcgcgctca acgaagatga ttcgatgcac cgttctggct ccatgcgcgc caggcgcgct
      361 gaattggcca cgaccagcag cgaactggcg gccatgccca ggcccgccag ccagggtggc
      421 atggccccgg ccaccgccag cggcacggac acggcgttgt acaccgcggc ccaggccagg
      481 ttttgccgca cgatggcgcg tgtgcggcgg gcgtgccgca tcagcgaggc cactgccgcc
      541 agctggccgc ccaggacaac gaagtccgac ttggcctgcg ccagcggcac ggcctggccc
      601 atggcgatcg agacatcggc gcgcgccagc accgggccgt cgttgacgcc gtcaccgacc
      661 atcgccacgc tttgcccgcg ctgctgcgcc agccgcacgt ggtccagctt gtcttcgggc
      721 gagcaaccgc cgaaggcccg ctcgacaccg gcgcggcgcg ccagccgctt gaccgcggcc
      781 gcgcgatcgc cggagagcag ttgcacctcc aggcccagct cgcgcagcgc gttcaccgcc
      841 gccggagcgt cgccgcgcat ggcttcgtcc agctcgaaac tggcgagcca gcccaggtcg
      901 tcggccaggt gcgactcaag acccgcggcg tcacgcgatc gggccggcgc gtcgcagaaa
      961 gatgccgatc ccagccgcag ttcacgcggc tgcccgccct cccccgccac caggcccatg
     1021 acgccgctgc caggcacctc ggcgacatgc gtggcggccc agccgacggc gccggcagcg
     1081 ccggcgatgg cgcgggaggc gggatgaagc gaatgccgcg ccagcgccgc ggccagcgcc
     1141 agcgcctcgg ccggctcggt gcccggccgg gcatgggttg cgaccagcgc catgcggtcc
     1201 tcggtgagcg tgccggtctt gtcgaacacg accgtgtcga ccgaggcaca ggcctccagc
     1261 gcctgcaggc gccgcaccag cacgcccgcg cgcgccaggg cgcccgcggc cgcgagcgtg
     1321 gccgccggtg tcgccaacga gagggcgcaa ggacaggtga cgatcaacac cgcgacggcc
     1381 acgcccaacg cggaacccgg gtcggtgggc caccaccagg ccgctgccgc ggcggccgcg
     1441 aggatgactc ccaccaggaa cgggcgggca atgcggtcgg cgatgcgcgc cagccggggc
     1501 ttgtccatcg acgcctgctc catcaacgcg acgatgccgg cgtagcgggt ctggctgccg
     1561 gtgcgctcca cccgaaccag caccacaccc gtcaggttgt ggctgccggc gaccacgggt
     1621 tcgcccttgc gccgcagcaa cggcctggac tccccggtga gcagggcctc gtccacgcgg
     1681 gtctcgccgt ccaccacgca gccgtcggcg ggaaaaacct cgcccggcag cacccggacc
     1741 aagtcgccgg cggccagccg ccgcaccggc acgcgctcga acgccccgtc ctcgaagcgc
     1801 tgccgctcta tcgtctgcgg caggcggcgc atcagcgctt ccagcgagcc cgccgtgcgg
     1861 tcgcgcagcc gctgctcgag caggcgcccc gacagcagga agaacacgaa catggtgacc
     1921 gagtcgtacc agacctctcg ccccagcgag ccgcccggat cgaaagtcgc ggccgtgctg
     1981 gcgccgaacg cgatggcgat gcccagcgcg accggcacgt ccatcccgat gcgcccgttg
     2041 cgcaggtcgc gcgccgccgc cgtgaagaag ggccagcagg aaaacagcac caccggcagc
     2101 gtgagcaccc aggaggccca gcgcagcagc gcctcgatgt cggacgtgat ctcgcccggt
     2161 gcggccgtgt atgccggaaa ggcatacatc atcacctgca tcatgcaaaa acccgccacc
     2221 agccagcgcc agagcagcag ccgctgcgcc tgccggcgcg gcgcggcggc aatcaggtcg
     2281 cctgccggga ccgcgccgta gccggcgcgc ttcagtgccg ccagccaagc cgacgggcgg
     2341 ctgctctggg gcgaccagac cagccgcgcg gtggcactcg cgccgttgac acgcacgctg
     2401 tcgacgccgg gcagttgcgc cagtgccgct tccacggtga gcgagcaggc cgcgcaatgc
     2461 atgccctcga tggcgaggaa cgattcccag cagccctcgc gtccggcgac ggcgcggctg
     2521 aagccctccc attccgcggg ctcatcgaga acggcccagc cgctcgcggg cgcggcgccg
     2581 gcggcgcgcg catcgatcgt caggacggca gtaggcatgc tgggaggcta tcggcagccc
     2641 ggggcacccc aattgacctg gatcaagcgg gggggccaag ccccgcccta agctgaacca
     2701 tcaacacgaa gggaacaagc aacatgtacc aaaaactcct tgtggcaacc gatggatcga
     2761 ccttgtccaa aaaggccgtg cgcagcgccg tcgaactggc aggcgccgtc ggcgccgaac
     2821 tggtggcact ttatgtcgtg ccccgctacc cggtcagcta tttcgaaggc gggatcaccg
     2881 tgtccacgca ggacatcgca cgcaccgaaa aacaatgggc cgacgagggc caggccgtgg
     2941 tcgacgccgt gcagcaggcg gcgcaagcca agggggtgaa ggccaagggc atcatcgcca
     3001 agtccgacct ggtggccgaa tcgatcatgg cgacggcgaa gaaacagaag tgcgacctga
     3061 tcgtcatggc ctcgcacggc cgcaagggct tcaagcgtat cctgctcggc agtgaaacgc
     3121 agcacgttct cacccacagc actgtgccgg tgctggtgct acgctgaccc gtttcgccag
     3181 gagagaacga tgaaaatact gttggccgcc gacggcagcg agtacacgaa gaaggccgcg
     3241 gcgttcctgg tcacccatga aaggctggcc ggtcccgatg acgagctgat cgtgctgcac
     3301 gtgcagtcgg ccgtaccgcc acgggtcaag gccatgctgg gcgcgagcgc catccgcgcg
     3361 taccacagcg aggaagccgc gaaagtccta tcgcccatcg agcggttcct gaagcgccac
     3421 cccctgaact tccgcaccct ctgggtggtc ggctcgcctg ccatcgaagt ggtgcgcgtc
     3481 gccaagcagg aaggcgtgca catgatcgtc atgggcacgc acggtcacgg catgctcggc
     3541 cgcgcgctga tgggcagcgt tgcccagaac gtggtcagcg agtgcgacat ccccgtgctg
     3601 ctcgtgaaat agcggcacgg gcaccgatcg ccgacggacg agcctgacat gcgaggcccc
     3661 tggaccctga ccgccaagct gtacaccagc ggcatcagct ttctcgtgct ggccctggcg
     3721 tcgatcggcc tgaccttgtg ggtcacatgg aacctggaag gcggcgctgc cgcggtcaac
     3781 gaggcgggcc gcatgcgcat gcagacctac cggctcgcgc tcgaagtgtc cggcaccgcg
     3841 gaccccgccg tcgtgctgag cctggcgcgc agtttcgacg aaagcctggc gctgcttcgt
     3901 gcgggcgatc cctcgcggcc cctgttcgtg ccatggtcgc gcgcatcgcg ggagtacttc
     3961 gacaaggtga acgaaagctg gggcgaactg cgcggccggt ggctggactc gcgtccgaca
     4021 ccggcccagg cggcccggga ggccggggcg ttcgtggcgc tggtggacgg gttagtggcc
     4081 gctatcgaaa gccagatcgg ccgctggaca gcgatcctgc acatgttcca gctggcgatg
     4141 atggggctgg ccatcggcag cgcggtgatg atgctttacg ccggctacct gttcgtgctc
     4201 aatcccctcg cccgattgca gcgcggcctc acggccgtgc agcagggcga cctgtccacc
     4261 cgggtcgaag tcgaaagcgc ggacgagttc ggccagcttt cggcgggatt caacgacatg
     4321 gcgcgcacgc tgcagtcgct gtacggcagc ctggaggaga aggtacgcga gaagacggcg
     4381 aagctggagg tcgagcgccg gcgtctggcg gacctgtacg aggtcagcgc gttcatggcg
     4441 catgccaccg ggctcgatga gctcgcgcac ggcttcgcgc gccaggtccg cgccgtggcg
     4501 caagccgatg ccgcggcggt gcgctggtcc gacgagggca accagcgcta cgtgctgctc
     4561 gccgcagaca acctgccgca gaacctggcc caggcggaga actgcctggc cacgggaacc
     4621 tgccactgcg gccaggccca cgccgacgcg gccattcgcg tgatcccgat ccgggcggcg
     4681 cggcccgcgc cgatggacca ttgcctgcgc tccggcttcc gcacgctggt gtcggtgccg
     4741 gtcaagctgc acgaacggat gctgggcgag gtggacctgt tcttccgcaa agaggtccgc
     4801 ctggaccccg aggaccgcga cctgctcgac acgctggcga gccacctggc cagcgccatg
     4861 gaaagcctgc gcgcgtcggc gctggagcgc gaggccgccg tcgcgcagga gcgcagcctg
     4921 ctggcccagg aactgcacga ttcgatcgcg cagtccctgg cattcctgaa aatccaggcg
     4981 cagctgctgc gcgacgcgct gcggcgaaac gataccgcct ccatgggccg cgtgatcggg
     5041 gagctcgatg ccggcgtgcg cgaaagctat gccgacgtgc gcgaactgct ggtgcacttc
     5101 cgcacccgca ccagcaagga ggacatcgag ccggcgctgc gcacgacgct ttccaagttc
     5161 gagcaccaga ccggcctgcc ggcccggctg gaaatggaag gccatggcgt ggccctggcg
     5221 ccggacgtcc aggtgcaagt gcttcacatc atccaggagg cgttgtcgaa cgtgcgcaag
     5281 cactcgcagg cccatgaggt ggcgttgcgc gtgtcctcgg acccctgctg gcgcttcgag
     5341 gttcacgacg acggcgtggg cttcgccgcg gacagcgcct cgtcgaacga aacccgcgtg
     5401 ggtcttcgca tcatgcagga gcgcgccgag cgcatcgggg ccaccgtgca ggtggcgtcg
     5461 gcgccgggca cgggatgcag cgtcgtgctc gaactgccgc gcgcacgggc ggaagctgcc
     5521 gccgcgtcgc cgataggtgc cgccgcatga atgggaccgg aaaggcggcc gtgcggctca
     5581 tggtggtgga cgaccacacg ctgttccggc gcggcctggt ggcgctgctc gcctccgaca
     5641 gcagcctgga cgtcgtgggc gaggcctcgg acgcgggtga ggccgtgcac aaggcggcca
     5701 cgctgcgtcc cgacgtcatc ctgctggaca accacctgcc cggagccacc ggcatccagg
     5761 cgctgcccgg gttgaaggaa gcggcgccgg cggcgcgaat catcctgctg acggtgagcg
     5821 aggacgagca ggacctgcag gcggcactgc gagccggcgc ccacggctac ctgctcaaga
     5881 ccatagaggg tgacttgctg gcgcagtcca tccatcgcgc catgcgcggc gagccggtca
     5941 tcagttccga gatgacgggc aagctggtaa cggccttcca gagttctgct tcaccgtccc
     6001 cgccggtgcc gagcccgccc gatccgctgg cccagctgtc ggcgcgcgag cagcaggtgc
     6061 tgcagcagat cgcgctgggc gcgagcaaca aggaaatcgc ccgcacgttg tcgatcgccg
     6121 agaccacggt gaagatccac gtgcagcaca tcctgcgcaa gctcgatctg acctcgcggg
     6181 tgcaggccgc ggtgttcgcc gccggcggct cgcaaaggcc gcaaggcgcc ggctcggacg
     6241 attcataggc gccccggcgg gcgctgaacc cgcatccctg cgcgttcctg gtcactgccc
     6301 gacatagttc caaaggagga tggcgacatc gcgtccatcc tccttctgca cagcgccagc
     6361 cgcaccaccg gaggatgttc ggcgaagcct ccagagtcca catttgaggc cgtgaaaaag
     6421 aactatcgga gcctcatctg atgcacagga aacatcaacc cccgcccgcc gatgccatcg
     6481 tcacccgcga atcctggtcg gttctggccg tcagcacgct ggcattcacc gtctgcttca
     6541 tggtctggat gatgttcggc gtgatcggca tcccgatcaa gaagacgctg aacctcaatt
     6601 cgaccgagtt cgggctgctc acggccatgc ccgtgctcac cggctcgctg atccgtgtgc
     6661 cgctgggcat ctggaccgac cgcttcggcg gccgcatcgt gatggccgtg ctgatggcga
     6721 tcacggtgcc ggcgatctgg ttcatgtcct atgccaccca gtactggcat tacctggtga
     6781 tcggcctttt cgtcgggttg gccggcggct cgttctccgt cggcacgccg tacgtggcgc
     6841 gctggttccc gaggaagcgc caaggcctcg cgatgggtat ttacggagcc ggcaactcgg
     6901 gggcggccgt caacaagttc atcgccccgg ccctcgtcgt ggcattcggc tggaccatgg
     6961 tgccccaggt ctatgcagcc gtgatgctcg gtacgctgct gctgttctgg ttcttcagct
     7021 acagcgaccc caagcacctg gtgtccagcg aggtgaagtt catggaccag ctcaggtcgc
     7081 tgaaggaccc caaggtgctg cgctactgcc agtactacag catcgtgttc ggcggctacg
     7141 tggccctgtc gctgtggatg gtgcagtact acgtcggcga attcggcctg gacatccgct
     7201 gggccgcgct cctcgcggcg gccttctctc tcccgggtgg cgtgctgcgc gccatcggcg
     7261 gctggctgtc tgacaagttc ggcgcgcaca gcgtgacgtg gtgggtgatg tgggtaagct
     7321 ggatctgcct gttcatgctg tcctacccgc agttcgaggt gaccttcctg accgtcaacg
     7381 ggcccaagac cttcgcgctc ggcttgaacg tgtacgcctt caccgcgctg atgttcatcc
     7441 tgggcgtcgc ctgggccttc ggcaaggcca gcgtcttcaa gtacatcagc gacgactacc
     7501 cgcacaacat cggcaccatc tcgggcatcg tcggcctggc cggcggcctc ggcggcttcg
     7561 tgctgccgat catgttcggc gcgctgatgg acatcaccgg cgtgcgctcc agcgccttca
     7621 tgctgatgta cggcgtggtc tggatttccc tgatctggat gtatttcacc gaggtgcgcc
     7681 gcaccgaggt catgggcgac cacgcccccg agtcgccgtc gctgcacctt ccgcacggcg
     7741 ccgcttcgcc aacctcccga gtgtccgccg ccatgccggc gcgggcctga tccgaacacc
     7801 cctaggagca cgttatgtct actgcattga tcgcccactg gaatccggag gacaagggtt
     7861 tctggcgcac acagggcgcc cccgtggccc agcgcaacct ctggatctcg attccggcgc
     7921 tcacgctggc cttcgccgtg tggatggtgt ggtcggtggt ggtcgtgaac ctgccgaccg
     7981 tcggcttcaa gttctcgacc aaccagctgt tctggctcgc cgccctgccc gccctgtgcg
     8041 gcgcgacgct gcgcatcttc tattcgttcg ccgtgccgat ttttggcggc cgccgctgga
     8101 ccgcgatctc caccgcctcg ctgctgctgc ccgccgcggg catcggcttc gcggtgcagg
     8161 accccgccac gtcctatgag gtgatggtgc tgctcgcctt gctgtgcggc ttcggcggcg
     8221 gcaacttcgc gtccagcatg gccaatatca gtttcttttt tccgaaggac cgcaagggct
     8281 atgcgctggg catgaacgcg ggcctgggca atctcggcgt gtccgtggtg cagttcgtcg
     8341 tgccgctcgt gatcaccgcc ggcgtcttcg gcaccatcgg cggcgacccg caagccatta
     8401 ccaaggcagg cgtgcagacc tcgatgtggc tgcagaacgc cggcttcatc tgggtaccct
     8461 tcatcatcgt gtccgcgctg gcagcgtggt tcggcatgaa cgacctggcc gacgcgaagg
     8521 cctccttcgc cgaccaggcc gtgatcttca agcgcaagca caactggctg atgtgctggc
     8581 tgtacctggg caccttcggc agcttcatcg gctattcggc cggcttcccg ctgctgatca
     8641 agagccagtt ccccggcatc aacgcactgc agtacgcctg gctcggcccg ctggtcggcg
     8701 ccctgatccg tcccgtgggc ggctggctgt cggacaaggt gggtggcgcg cgggtgacat
     8761 tctggaactt catcgcgatg gcgctcggcg tactggcggt actgcagttc ctgccggcca
     8821 atggccaggg cggcaatttc accggcttcc tcatctcgtt catgctgctg ttcgccacat
     8881 cggggatcgg caacggctcc accttccgca tgatccccgt catcttcatg accgagcgcc
     8941 agcgcgcggc cgccaagaac gcccctgcgc aggaacaggc cgtgaaggac gccaacaagg
     9001 aagcggccgc cgtgctgggc ttcagctccg cgatcggcgc ctatggcggc ttcttcattc
     9061 ccaagagcta cggcacctcc atcaccatga ccggcgggcc tgaagccgcg ctgtacacct
     9121 tcatcgtctt ctacgtgact tgcgtcgcta tcacatggtg gtactacagc cgcaagaacg
     9181 cgccgatgcc gtgctagatg cacccacccc cgaagcgcct tcggcgatgc cccctcgggt
     9241 ttcgccctct ccccctcaag ggggcgcgac cagcggaccg gcaaagccgg ttccgcggtg
     9301 aaggcccacc cccgaagcgg cctgcggccg cctccccctc aaagggggcg ccaccagcgg
     9361 cccggcaaag ccggttccgc ggtggccccc gaactgcatg cctgaacgac cgagaaatcc
     9421 acgatgagcc atttcctcga ccgactgacc tacttttcac agccccgcga gccgttctcc
     9481 ggcgaccacg ggcagaccac cgaagaagac cgcacctggg aagacgcgta tcgcgcgcgc
     9541 tgggcgcacg acaagatcgt gcgcagcacg cacggcgtga actgcaccgg atcgtgctcc
     9601 tggaaaatct acgtcaaggg cggcatcgtc acctgggaaa cgcagcagac cgattacccc
     9661 cgcacccgct gggacatgcc caaccacgag ccgcgcggct gcgcccgcgg cgcgagctac
     9721 agctggtatc tctatagcgc caaccgcgtg aaatacccga tggtgcgcgg gcgcctgctc
     9781 aaggcctggc gcgaagcgcg ccgcagcatg ccgccggtgc aagcctgggc cgcgatcgtc
     9841 gccgacgatg ccaaacgcaa gggctggcaa agcgtgcgcg gcctgggcgg cttcacccgc
     9901 tcgagctggg acgaggtcaa cgaaatcatc gccgcggcca acatccacac catccgccag
     9961 cacggtccgg accgcatcat cggcttctcg ccgatccccg ccatgtcgat ggtcagctac
    10021 gcggccggca gccgctacct gagcctgatc ggcggcgtct gcatgagctt ctacgactgg
    10081 tactgcgacc tgccgccggc cagcccgcag atctggggcg agcagaccga cgtgcccgag
    10141 tcggccgact ggtacaactc cagctacatc atcgcctggg gctccaacgt gccgcagacg
    10201 cgcacgccgg acgcccactt cttcaccgaa gtgcgctaca agggcaccaa gaccgtggcg
    10261 atcacgcccg actactccga agtcgccaag ctctccgacc tgtggatgca cccgaagcag
    10321 ggcaccgacg ccgcggtggc gatggcgatg ggccacgtga tcctcaagga gttctatttc
    10381 gactacgggc gacgggccgc ccctagcccg gacacccccc ccgggggggc ggacgctgtg
    10441 aggcggcctg gggggcgaaa tacttcggcg tacttcgacg attacgcgcg ccgttacacc
    10501 gacctgccga tgctggtgat gctcaaagag cacaccctgc cttcgggcga aacggtcatg
    10561 gtgccggacc gctacgtgcg cgcctccgac ttcaacggca aactgggcca gtcgaacaat
    10621 cccgagtgga agaccgtcgc gatcgccgac gacggcaagg tcgtgctgcc caagggcgcc
    10681 atcggcttcc gctggggccc ggatggccgc ccggaccagg gccagtggaa cctggaagac
    10741 aaggaagcgc gccacgacca gcaggtcaag ctcaagcttt ccctgctgga gggcgaagcg
    10801 ccgagcaccg agacggccaa gatcggcttc ccctatttcg gcggcatcga gacggcgcat
    10861 ttccaacaca acacccagac cgacgtgctg gtgcgcaccg tgccggtgca gcgcatcgcg
    10921 ctgggcaagg ccggcgagga acgctccgcg ctggtggcca ccgtgttcga cctgcaggcg
    10981 gccaactatg gcgtgtcgcg cggcctgccc ggcgaactcg ccgccgagag cttcgacgac
    11041 gacaccccgt acaccccggc gtggcaggaa aagatcaccg gcgtgccgcg cgaccagatc
    11101 atcaccgtcg cgcgccagtt cgccgacaac gccgacaaga cgcatggcaa atccatggtg
    11161 atcatcggcg cggcgatgaa ccactggtac cacagcgaca tgaactaccg cggcgtcatc
    11221 aacatgctga tgatgtgcgg ctgcatcggc cagtcgggcg gcggctgggc gcactacgtg
    11281 ggccaggaaa agctgcgccc gcagaccggc tggaccgctc tggccttcgc gctggactgg
    11341 atccgccccc cgcgccagat gaactccact tcgttcttct atgcccacac cgaccagtgg
    11401 cgctatgaga aactcggcat ggaagaagtg ctgtcgccgc tggccgacaa gaaagctttc
    11461 gcgggcagca tgatcgacta caacgtgcgc gccgagcgca tgggctggct gccgtcggcg
    11521 ccgcagctgc agaccaaccc catgcaggtc gtgcgcgacg cccaggccgc cggcatggat
    11581 ggcaaggagt acacggtcaa ggcactcaag gacggaacct tgaagatgtc atgcgaggac
    11641 ccggaccatc ccgccaactg gccgcgcaac ctgttcgtct ggcgctccaa catcctgggt
    11701 tcctcgggca agggccacga gtacttcctc aagcacttgc tgggcaccag ccacggcgtg
    11761 cagggcaagg atctcggcgc cgacgaggcc aagccgacgg aggtggtgtg gcacgacaag
    11821 gcgccggaag gcaagctcga cctcgtggtc acgctggact tccgcatgag cacgacctgc
    11881 ctgtattcgg acatcgtgtt gccgacggcg acctggtacg agaagaacga cctcaacacc
    11941 agcgacatgc atccgttcat ccaccccttg tccaccgcgg tggacccggc ctggcagtcc
    12001 aggagcgact gggaaatcta caagggcttc gccaagaagt tcagcgagct gtgcccgggt
    12061 cacctcggcg tcgagaagga agtggtgcta tctccgctga tgcacgactc gcccgctgaa
    12121 ctggcgcagc ctttcggcgt gtccgactgg aaacgcggcg agtgcgagct gattcccggc
    12181 aagacggcgc cgcagatcac ggtggtcgag cgcgactacc ccaacgtgta caagcgcttc
    12241 acggcgctgg gcccgctgat gaacaaggtc ggtaacggcg gcaagggcat cgcgtggaac
    12301 acgcagacgg aagtcgagca gctcggccag ctcaacggcc tggtcaccga tgccgggcaa
    12361 acgcagggca tgccgaacat cgataccgac atcgacgcct gcgaggtcgt gctgcagctc
    12421 gcccccgaga ccaacggcca tgtcgccgtc aaggcctggg aggcgctgtc caagcagacc
    12481 ggacgcgaac acgcccacct ggcgctgcac cgggaggacg agaagatccg cttccgcgat
    12541 atccaggcgc agccgcgcaa gatcatctcg tcgcccacct ggagcggcct ggagagcgaa
    12601 aaggtctcgt acaacgccgg ctacacgaac gtgcatgaac tgatcccgtg gcgcaccctg
    12661 acggggcgcc agcagttcta cctcgatcat ccgtggatga gcgcgttcgg cgaagcgttt
    12721 gccagctacc gtccgcccgt ggacttgaag accacggccg gcatcgcggg catcaagccc
    12781 aacggcaaca aagagatcca gctgaacttc atcacgccgc accagaagtg gggcatccac
    12841 tcgacgtaca gcgacaacct gatcatgctg acgctcaacc gcggcggccc ggtgatctgg
    12901 ctgtcggagg acgacgcgaa gtcagccggc atcgtggaca acgactgggt cgagctgttc
    12961 aacatcaacg gcgcgatcgc cgcacgggcg gtggtcagcc agcgggtcaa tcccggcatg
    13021 gtgctgatgt accacgccca ggaaaaaacc atcaacacgc ccggctccga gatcaccggc
    13081 atgcggggcg gcattcacaa ctcggtgaca cggatcgtgc tcaaacccac ccacatgatc
    13141 gggggctacg cccagttcag ctacggcttc aactactacg gaaccatcgg caccaaccgc
    13201 gacgagttcg tggtggtgcg caagatgaac aaggtggatt ggctggatga ggaggtggcg
    13261 gcatgaaggc ggtatctctc cctctcccct ccggggagag ggctggggtg aggggcacac
    13321 gctcccactc ccgccctcac cctaaccctc tcccagcggg agagggaaca agtcctcagg
    13381 agtacaagaa atgaaagtcc gcgcacaaat cggcatggtc ctgaacctgg acaagtgcat
    13441 cggctgccac acctgttcgg tcacgtgcaa gaacgtctgg accagccgcc ccggcatgga
    13501 gtacgcgtgg ttcaacaacg tcgagaccaa accgggcatc ggctacccca aggagtggga
    13561 gaaccagggc aagtggaacg gcggctggaa gcgcaacgcc gacgggtcca tcgagccgcg
    13621 ccagggcaac aagtggcagc tcctgatgcg tatcttctcc aacccgaacc tgccgcagat
    13681 cgacgactat tacgagccgt tcaccttcga ctacgaccac ctgcagtcgg cgcccgaaat
    13741 gaaggcagcg cccaccgcca ggccgcgcag cctgatcacc ggccagcgca tggaaaagat
    13801 cgaatggggc ccgaactggg aagaaatcct gggcggcgaa ttcgccaagc gcggcaagga
    13861 cgcgaacctg gcgaatttcg acaaggtgca gaaggacatg gtgggccagt tcgagaacac
    13921 cttcatgatg tacctgcccc gcctgtgcga acactgcctc aacccggcat gcgtcgcttc
    13981 ctgcccttcg ggctcgatct acaagcgcga ggaagacggc atcgtcctga tcgaccagga
    14041 caagtgccgc ggctggcgca tgtgcgtctc gggctgcccc tacaagaaga tctactacaa
    14101 ctggcagtcc ggcaaggccg agaagtgcat cttctgctac ccgcgcatcg aagccggcca
    14161 gccgacggtg tgctccgaga cctgcgtggg ccgcatccgc tacctgggtg tggtgctgta
    14221 cgacgcggac cgcatccggg aagcggccag cgtggaaaac gaaaaggacc tgtatcaggc
    14281 ccagctggac atcttcctcg acccgaacga ccccaaggtg atcgagcagg cgcgcaagga
    14341 cggcatcccc gaagcctggc tggaaggcgc ccgcaagagc ccggtgtgga agatggcgat
    14401 ggactggaaa gtggcgctgc cgctgcatcc ggaataccgc accctgccga tggtgtggta
    14461 cgtgccgccg ctctcgccga tcacgtcggc cgccaacgcc ggtcatctgg gcgccaacgg
    14521 cgagatcccg gacgtgcagc agatgcgcat cccggtgcaa tacctggcca acctgctcac
    14581 cgccggcgac accgggccgg tgatccgctc gctggaacgc atgctggcca tgcgcgcctt
    14641 ccagcgcggc aagcacgtgg accgggtcga gaaccgcgcg gtgctcgatc aggtcgggct
    14701 gacggtgggg caagtggaag acatgtacca ggtgatggcg atcgccaatt acgaagaccg
    14761 cttcgtgatc ccgagcacgc accgcgagta tgccgagaac accttcgaca tgaagggcgg
    14821 ctgcggcttc tcgttcggca acggctgctc cgacggcagc accgagacga gcctgttcgg
    14881 cggcaagaag aagcggacga ttccaattca agtggagaag gtatgagcga caacaacaac
    14941 ccgaagctcg tcatgccgga cttgatccgg catccagcaa cggggcttcg acaagcgcag
    15001 tcctggattg cgggtcaagc ccgcgatgac aagctcgtac gacataccct gcgctcactg
    15061 gccttgctgc tgggatatcc cgacgaaaaa ctgcgcggcc tgctgccggc cctggccgac
    15121 gcgatcgacg ccgagaaggc cttgccgtcc tcgcggcgca tcgaactgcg cgccttcatc
    15181 ggcgagatgg aacgcgccga cccgatcgag ctggaagcgc gctacgtgga gctgttcgat
    15241 cgcggacgcg ccacctcgct gcacctgttc gagcatgtgc acggcgactc gcgcgatcgc
    15301 ggcccggcca tgatcgacct gacccagacc tacgagaagg ccggcctgct gctgggcccg
    15361 cacgagctgc cggatcacct gtgcgtggtg ctggaattcg cttccaccca gccgcccgcg
    15421 ctggcgcgcg aattcctcgc ggaaatgggg cacatcctca ttgcgatctt cagcgcgctg
    15481 cgcaagcgcg aaagccgcta cgccactgtg ctcgcggcag tgctggaatt ggccggccag
    15541 aaggtcgagg ccgtgcccgt ggtagacgac gagccgattg accatgcatg ggccgagccc
    15601 gtggtgttcg acggctgcag cacccgcggc caggcagtgc ccggccagcc ccaacccatc
    15661 cacatcgtcc gcaagacccc tcccgtcgaa ggagcacgcg catgaactac ctgaatcaat
    15721 tcctgttcgg ctactaccct tatatctgcc tcgttgtctt cctggccggc agcctgatcc
    15781 ggttcgatcg cgaccagtac acctggaaga gcgattcatc gcaattgctg cgccacggcc
    15841 agctgcgctg gggcagcaac ctgttccaca tcggcatcct cttcctgctc gcgggccatg
    15901 gcgcgggcct gctgacgccc cacttcatgt acgagaattt cgtcagcgcc ggcgacaagc
    15961 aattgacggc gatgatctcg ggggggctgt tcggcctgct ggggttcatc ggcgtcacga
    16021 tcctgctaca tcgccgcctg acggacccgc gcatccgcgt caactccaag acaagcgaca
    16081 tcatgatgct ggtgctgctg tgggtccagt tcgcgctcgg cctcgccacc attccgttgt
    16141 cggcccagca cctggacggc agcctgatga tcaagctggg ggaatgggcg cagcgcatcg
    16201 tcacgttccg gccgggcgct gcggaaatgc tggtggacgc gggctgggtg ttcaaggccc
    16261 acatgttcct gggcatgacc ttgttcctgg tctttccgtt cacccgtctg gtgcacgtgt
    16321 ggagcggctt cgcgaccctc ggctacctgg tgcggccgta ccaggtggtg cgcacccgcc
    16381 gcaagatcgc gccgtcgcgc cccgtcgccg tggcacccgc cgcatcgacg acccagccgg
    16441 tcgcgggcgg catgctcgaa ccgggaaagg ccacttcatg agtgaaatga ccggatgcgg
    16501 cggcggcgcg tgcacctgcg ggggcggcgc ccaccccgtc aacggccgag cgctcaccgc
    16561 cgagccgccc gcgccgcccg ccaggatcaa tggcatcgcc ttgcatccgg ccgggctacg
    16621 gccggacgaa gccggcctgc gcgagctggc ctgggccgaa ttgctgcggc aggaagccgt
    16681 gcgccagggc ctgctgcccc ggcaaccggg cttcgaggca ccgcagctgc tacccggcga
    16741 ccggcaggtg atccaagcca tgctggaacg cgcggtccaa gtcccgcaac cctccgagca
    16801 ggaatgccgg cgctactacg aagcccggca ggatcactac gtcgaagggc gcatggtcaa
    16861 cttgcgccac atcctgttcg ccgtcacctc gggcgtggac gtccatgccc tcgcggtacg
    16921 ggccgaaacg gcgctgctgg aactcagccg gcaaggcgtg gatcccggcc gttttgcggc
    16981 actggcccga gagctgtcca actgccccag cggcgctgaa ggcggcgacc tgggctggat
    17041 agcgccgcag gactgcgcgg acgagctagc caacgagctc ttccaccaga agaacccctt
    17101 gcgcggtatc ggccttcgcc cccgtttggt gcacacccgg tacggattcc atatcgtcga
    17161 ggtgttgggc cgcaagcagg gccggctatt gtcgttcgac cacgtccgcg agcgcattgc
    17221 cgtgcagctg gcccagcagt cgcgtgccaa ggcgctgcac cagtacatcc agctgctcgc
    17281 gggccaggcc agcgtcgaag gggtattgct ggaagccacg gattcgctgc tggtacagta
    17341 aagaaccagg ccccgaaccg acacggaccg ctgcgaagaa accattctcc ttgatgaata
    17401 gcaaggaggt gacccgccgg gttcggtagc ctcctacggt ggccccgggg gccatgcatt
    17461 cttttggagc acgtttggac acattctcgt tggctgacgc cgcggcggtc gccgcgcggc
    17521 tgcacgctct gtgcagcgac tcgggcctgc gcccatggca attcgcgggc aagtcgctgc
    17581 tccccgtcgt gcagggcggc atgggcgtcg gcatttccgc gggcggcctg gccggcgcgg
    17641 tggcggcgct gggcgccgtg ggcaccattt cctccgtgga cctgcggcgc caccaccccg
    17701 acctgatggc cctgaccgag aatctggacc aggaaccgga cgcccgcgag cgcatcgacg
    17761 ccgccaacct cgaagccctc ggccgcgaaa tcgcgcgggc gcgcaaactc tccggcgggc
    17821 gcgggctgat cgcggtgaac atcatgcgcg ccgtgagcgc ctacgaagcc tatgtgcggc
    17881 attccctggc ctgcggcgtc gacgccgtcg tggtgggcgc gggcctcccg ctggacctgc
    17941 ccgagctggc gcgcgattac ccgaaggccg cgctgatccc catcctgtcg gacgcacgcg
    18001 gcgtgcagct ggtcgtgcgc aaatgggaga agaagggccg cttgccggac gccattgtca
    18061 tcgagcaccc gcgcctggcc ggcggccacc tgggcgccgc caaggtagcg gacctgaacg
    18121 accgccgctt cgatttcgac gtggtcttgc ccgaggtgct ggctttcctg cgtgcgtcgg
    18181 gtctggaggg ccgcattccg ctcgtcgccg ccggcgggat cggcagcctg gaggatatcc
    18241 ggcgactgca ggacctgggc gccacggccg tccagctggg cacggccttt gccgtcacca
    18301 ccgagtgcga cgcccatccc gagttcaagc gaatcctggc cgaggccggg ccggaagata
    18361 cggtcgagtt caccagcgtg gccggcctgc ccgcgcgcgc cgtgcgcacg ccctggctgg
    18421 acaaatacct gcgcatcgag tcgcgcctga aaggcaacgc gaaagtaaag ccctgtacca
    18481 tgcgcttcga ctgcctggcg cattgcgggc tgcgcgacgg caacgcggcc tggggccagt
    18541 tttgcatcga caagatgctg ggccacgcgc tcgccggcca gaccgggctg ggtttgtttt
    18601 tccgcggcgc gggccgcttg ccgttcggct cgcagatccg ctccgtccat gacctgatgc
    18661 aatggctgat gggcggcatc cgcccggccg agctgttgga gaactgcgcg ttggagactg
    18721 tgcatgaagc gtgacgaagc cgccctggtc cccttggcgg tccagcccat cagccgggac
    18781 gtcctcgcgg agaaatacct caaacccggc gaaaaggacc tcgaggacct gttcgcgcgg
    18841 gttgctcgag ccctggcgtc ggtcgaaacc gaatcccagc gcgatcactg ggaagccctt
    18901 tttcacgcga acatgcgtgc cggcgccatc ggtgccggcc gcatcatgag cgccgccggc
    18961 accacgctgg aggccacgct gatcaactgc ttcgtccagc cggtgggcga ctgcatccag
    19021 ggaatggacg cggacggcta ccccggcatc tacgaggccc tgcgcgaagc ggccgagacc
    19081 atgcggcgcg gcggcggcgt gggctacgac ttctcgcgca tccgcccgcg cggcgccgag
    19141 gtgaaggcca ccgcctcgat cgcgtcggga ccctgcagct acatcgacgt gttcgaccat
    19201 tcgtgcgcca ccgtggaaag cgccggcgcg cgccgcggcg cgcagatggg cgtgctgcgt
    19261 atcgaccacc ccgacgtgct ggagttcatc accgccaagc gcaagccggg gcgctggtcc
    19321 aatttcaatg tctcggtcgc ggtgacgcat gccttcatgg aggcgctcga cgccggcgcc
    19381 gcctggtcgc tggtgcatcg cgcgcggccc ggcgcggcgc tgctcgaaaa aggcgcccag
    19441 cagcgcgagg acggcatgtg ggtctaccgc acactgcccg ccgccgaact ctgggacacc
    19501 gtgatgcgct cggcctacga tttcgccgag ccgggcatcc tctttcccga caccatcaac
    19561 cgcgacaaca acctgcgcta ctgcgagaac atcgcggcga ccaatccttg cgtgaccgcc
    19621 gacacctggg tgctgacgac cgatggccct cgccaagtgg tcgacctgat cgggcgatcg
    19681 ttcgacgcgg tggtcgacgg caaggcctgc cgagtcgaaa gccagggttt cttcaagacc
    19741 gggcacaaac ccgtcctgtc gctgtgcaca cgcgagggcc attgcctgcg cctgaccgcc
    19801 gaccatccgg tacggcgcgt ggcacgcaag agccgctaca cgcttcaagc cgagtggacg
    19861 ccggctgcgc agttgcgacc cggtgatgag atcatcctgc acgaccaccg ggcattgggc
    19921 ggctgggacg ggcgcggcac cgaggccgaa ggctacctgc tcgggttgct tgtcggcgat
    19981 ggcacgctca agagcgacaa ggccgtcatc tgcgtctggt cgcccgaact gcgtcaggtg
    20041 gcgcagggcg cgcccgccta cgcggcaacc ggcgcgagcg gcatcatgca agcggccgaa
    20101 gcggcagcgg ccacgttgcc gcatcgcgcc gatttccgcg gcttccagcg gcccgttgcc
    20161 gggcgcggcg aagcgcgcat ggcgagcgcc gccgtgtggc acctggcgca cgacatgggc
    20221 atgcggccgg gcttcaagac cgtgacgccc gacatggaga agtcctcgtc ggcgttctgc
    20281 gtgggcgtca tgcgtggcct gttcgacgcc gacggctcag tgcagggttc caccgaaata
    20341 ggcgtgagcg tgcgcttggc gcaaagcgac ctgccgctgc tgcaggcggc acaacggatg
    20401 ttgctgcgcc tgggcgtcgc atccacgatc tacacgaacc ggcgccccgc tctcgcgcgg
    20461 ttgatgcccg acgggtccgg cggctccagg acttacgaca cccgggccca gcacgaactc
    20521 gtgatcagtg gcgacaacct gcgcgcgtat gccgaacgga tcggctttgc cgacaccgac
    20581 aaggcggcgc ggctcgaggc ggtcctgggc agctacagcc gagccctcaa tcgcgagcgc
    20641 ttcacggcga cggtcgagtc cctgactgaa ggcgcgcacg aggacgtgta cgacgtcacc
    20701 gtggctgatg tccacgcctt cgacgctaac ggcattttcg tccacaactg cggtgaacag
    20761 ccgctgccgc cctacggctg ctgcgacctg gggccggtga tcctgacgcg cttcgtgcac
    20821 cagccgttcg gcttcggcgg aacacccgaa ttcgacttca aggcgtttgc cgcctcggtg
    20881 gcgctgcagg tgcgcgccct ggacaacgtg ctggacctga cgttctggcc gctgccgcag
    20941 cagcgggccg aagccatggc caagcggcgc atcggcgtgg gttttaccgg catgggcgac
    21001 gccctgtcca tgctgtgcct gcgctatgac gagccggccg gccgcgacat ggcggtgcgc
    21061 atcgcccgct gcatgcgcga cgccgcctac ggcgcgtccg ttgcgctggc gcgcgagaag
    21121 ggggcattcc ccacgttcga cgcgcagcgg tacctggaat ccggcacctt cgccagccgg
    21181 ctggacgagc cgctgaagaa ggagattcgc gaacacggca tccgcaacag ccacctgctt
    21241 tcgatcgcgc caacgggaac cgtcagcctg gccttcgccg acaacgcctc caatggcatc
    21301 gagcccgcct tctcctggac ctacacgcga cgcaagcgcg agggcgacgg cagcgtcacc
    21361 gagtacgacg tcgaagacca cgcctggcgc ctgtatcgca ccctgggcgg cgagatggcc
    21421 aagttgccgc cgtatttcgt gtccgcaatg gagatgccgg cggctgccca cgtcgccatg
    21481 atggaggccg tgcagccctt cgtggacacc gcgatctcaa agaccgtgaa tgtctccgag
    21541 gactatccgt acgaggattt caagggcctt tatcacgagg cctggaaggc gggcctgaag
    21601 ggcctcgcca cctaccggcc caacagcatc gtcggcgcgg tgctgcaggc agaggcgacc
    21661 aaacccgcag ccgaagcgca gccggccccg atggacctca tgcgcaccgt catcgccagc
    21721 cgcccgaagg gcgcgctgcc ggcgatggcc gaaaagatcg agtactggac gcagcaaggc
    21781 cgccagcacc tgtacctggt ggtgtcgttc ctgcctctgc cggacggccg gcagcgtgcc
    21841 atcgaattct tcatgccggt gggccagaac ggagagtcgc agcagtgggt gaccgcgagc
    21901 atgcggctgc tgtcgctcgc ggcgcgcggc ggatttctcg accgcgcgct ggccgacatg
    21961 cgcaaggtcg cctgggatcg cgggccggtg cggctgggca cctatcgaaa ggccgacggc
    22021 acgcatgtcc cgctgtggca cgactcggaa gtcgccgcga tcgcgtacgc cctgcagaac
    22081 ctgatcgccg ggcgcaacgg gcacgagaac gaggcgcccg ccgccgtcat cagcgcccct
    22141 gctcccgcgc caatgaccgg tgccaagtgc cccgaatgcg gcgcctacgc gatgatccgc
    22201 aaggacggct gtgactactg cacgcaatgc ggtcacctgg gcgcttgcgg atgagatgaa
    22261 agcccacccc cgaagcgcct tcggcgcctc cccctcaagg gggcgccacc agcggcccgg
    22321 caaagccggt tccgcggtgg cccccgcgtc gatcgcgcca attcatgcgt cacgggtcgc
    22381 gtcaagcgct gacggaaccg cgcgcgattc gcatcggcac gctgacaccc tcaactgaac
    22441 gcagatatgg cgcctggcaa ggaggacgcc cgagttccga cccccttcat gggtgagccc
    22501 ggttccgccc aatgggcggc gttgctcatc cagcatcgcc agaccatcct gcccaaacgc
    22561 ctggcggaac cgggaccgga cgccgcccag ctcgagctca ttttcggcgc cgccgcggcg
    22621 gcgcccgacc acggcgaact ggtgccgtgg cgtttcgtca tcattccggc cgaggcgcgc
    22681 gagcggttgg cggacgtgtt tgcggcttcc ctgctcgagc gcgactcgag cgccacggcc
    22741 gaagaggtcg cgaaagcgcg cgagaaggcc tatcgctccc ctctcctcct gcttgccgtg
    22801 gtggatgtgg ggccccccgc cgacgaagtt tcgcctgcgg agcggatgat ctcggccggc
    22861 tgcgccatcc agaacatgct cctgatggcg acgaccatgg gcttcggctc cgcgctcacc
    22921 agcggcaagg ccttgcagtc gcagggcctg cgtgagatgt tttcgttgcg ggagtgcgaa
    22981 caggccgtat gcttcgtcag catcggtacc acgctgcgcc gcaagccgcc gcgcctgcgg
    23041 ccggagccca gccgctacgt cagcgttctc gcgccgcccg ctgcatgagc caacccatac
    23101 gaatcacatc gctggtctcc gggccggccg ccggcttcga gcagccgttc gagatgctag
    23161 aggcctgcca cgagcgcgtc caacgcatgc tggcgctgct gcagcgcctg cgcgagcacc
    23221 tgcccggcca cggcgcggat gccgacgcgc ggcaggccgc gcgcgacgtc atgcgctatt
    23281 tcgaccaggc cgcgccgcag caccaccgcg acgaggagct tcatgtgttc ccgccattgc
    23341 tggcccaggg cgatgtggcg acgcaggacg tggtcaggcg gctgcaacag gaccaccttc
    23401 agatggagcc gcgctgggcc gcggcccgcg ccgtgctcga tgcggtggcc ggcggcgagc
    23461 ttgcggcatt gctgagcgcg cacgaggccg cgctcgatgc ttttgccggt ccctacgccg
    23521 accacatcgc ggccgaggag cagcacgtct atccggcagc ggcggcgttg ctcgacgaga
    23581 aggccgtggc ggcgatgggc gcggagatga tgcgcaggcg cggcgtggcc tgaagctgcc
    23641 tctaccgaat gcggggtgtc gcctccatct gttcgggcgt ggtgaaatag gccgacgagg
    23701 cgccgcctcg ctcgcgcgcc cgggccaact cctgccgcgc caccgttcgc aagtggacct
    23761 cgtcggggcc atccaggaac cgcatcgcgc ggccccaggt ccacagccac gccagcggcg
    23821 tatccggcga cagccccatc gcgccgaaga tctgcatcgc gcggtcgacc acccgtgtct
    23881 gcagccgcgc ggcaaccacc ttgatggccg cgacgtcggc acgcagcctc tgtggccgct
    23941 gcgcctgatc caggccccac gcggcgcgca gcaccagcag ccgggcctgg tcgatctcca
    24001 ggcgcgactc ggcgatccag tcctgcacgt tcgactggtc cgcaaggtac ttgccgaagg
    24061 cgtgccgctc cagtgcgcgg tcgcaggcca gctccagtgc cagctcgcac tggccgatcg
    24121 tgcgcatgca atgatggacc cgcccgggcc ccaggcgccc ctgcgccatc gcgaagcctt
    24181 caccccagcc cccaaggagg ttttcgtccg gcacgcgcac gtcgcgcagc acgatctcgc
    24241 aatggccttc gggcgaggtg tgttgcatga tcgggatatt gcggatcacg ttcacgccgg
    24301 gcgtatcgat cggcaccaat accatgctgt ggccggcgtg ccgcgatgcc gtttccggct
    24361 caccgtcgtt gcggcacatc acgatcagca gccggcatgc ggggtgcgcc acccccgaga
    24421 tgaaccactt gcggccattg atcaccagtt ggccgccctc gcgccgcacg gccgtctgca
    24481 ggttggtcgg gtcggacgaa gccacatccg gctccgacat ggcgaacgcg gagcggatct
    24541 ccccttccag caacggcagc agccagcgct tgcgctgatc gggggacgca tgccgctgca
    24601 gcaggtccat gttgccgctg tcgggggcgc tgcagttgaa gacctcggcc gaccagggca
    24661 gccggcccat cgcttcggcc agcggtgcat agtcgaggtt gtccaggcgc gtgcccggct
    24721 cgtcctcgcg caggcccggc aggcacatgt tccacagccc ttccgctcgc gccagggcct
    24781 tgaggtcttc caggaagggc gggtactcgc cctgcctcgc gcagcgatgc caggcgccgt
    24841 tgtgcgggag cacgaagcgc tcgatgaatg catcgagctg ccgcaggcgg gcaatggcgg
    24901 tgggggatgg ttcgaagttc attaggggcc cgttcacact ccaggccagc ttaggggcgg
    24961 gccgctcgcg ccgggatgat ctccgtcaac gtttgcggcg cgcagcgctc cggatgtcac
    25021 ttaggagtca aggaggcagg ggctggaggc ggggcgttac gcgacgaccg tgaccagcat
    25081 gttggcgtcc ttgcgcacca cctcgatgcc gacgtttcgc tcataccgct gcagcagcag
    25141 gtcggcgctc gggcccggcg cgcccagccg ggtgatcctg atccagtcga tgaacggcgg
    25201 cagccggggc gtgcgcagcg tgatctcgga cttgccgaca tcgatgtcga gtccgagaca
    25261 ggcctgcaac atgccgaaca cggcggccgc ggcccaagcc tggggcgaac acgcgaccgg
    25321 gtacagggtg ggtcccgcac ccgggcgccg cgggaagccg cagaacaatt ccggcagccg
    25381 gtgcgcgtcg aaatgcaggc tggcgtcgaa cgtgctcgac agcagttgca tcgcgctttc
    25441 ggtgcgcccg tagcgcgcca ttcccgccgc gatcagggcg ttgtcatggg gccagatcga
    25501 gccgttgtgg taggacatcg ggttgtagcg tggcgctccg gcggggatcg tccggatgcc
    25561 ccaaccgctg aaaaaccgat ggcccatcag gccatcgacg atgcgcggcg catgttcggg
    25621 cgacgcgatc ccgctccata gcgtgtgccc cgcgttcgaa gacgcgacct tgcattgctg
    25681 cttgttgcca tccagcgcga tggcgtagct gccgatttcc tcgcaccaga aagcggcgtg
    25741 gaaacgctgg cgcaactgcc gcgcctgcga ctccagcgcc tgggccagct gaggctcgtc
    25801 catgaggcgg gccagttcgg cggccgccag cttgccttcg tacacgtagc cctgcacctc
    25861 gcacagtgcg atcggtgggc cggccaaccg cccgtcctgg tggaatatgg aatcgccgga
    25921 gtccttccat ccctgctgca ccaggccctc gtcgctgcgg cgcgagtatt ccacgaagcc
    25981 gtctgcgtcg gcatcgcccc actggtcgac ccattgcagg gccatccgga tgttgggcca
    26041 gaggctgcgc accagcgccg cgtcgccggt gcgcttgtaa taggctgccg cgagcaccag
    26101 gaacagcggc gtcgaatcga ccgaaccgta gtagcgcccg aacgggattt cccgcgtcag
    26161 cgccatctcg ctgtgacgcg cctcgtgcag tatcttgccc ggctcggcgt cgttctgcgg
    26221 gtccagctcc gtcgcctggt tggccgcaag gtaagtgagc acaccacgcg ccagcgaagg
    26281 gtccacccag aggtattcga gcgcggtgat gatgccgtcc cgcccgaagg tggtggagta
    26341 ccacggcacg ccggcgtagg ggtacgggcc ggtcggcagc atggtggtca gcatcgacag
    26401 gtcggacacg gaccggtcca gccacaggtt gaccagcggg ttggacgtga ccacggtgca
    26461 gtggccgccc agccaggccc tgcgcgcctc gttggcctcg cgcaatgccg cttcatacga
    26521 aatgcgcgtg ggcgggggcg cgttgtcgac ctcgcacagc actgtgcagt agagatggtg
    26581 ttcggtaccg gggtccagcg ccagttcgaa gacggcccgc cccgcgtcga tggaactcgg
    26641 cgcgggctcg aaatggatcc gcgtgcggcg caggtgcttg tcgcggccga ggtaggggag
    26701 caccacttcg tgcgccatca cctccggctg cagccggtcg ccgcgccgtt cgcgcttcat
    26761 gccgcgcacc tcgaacaggt cgacgaaatc cgcgtcgaac gtgaattcca gcctgacact
    26821 ggtgcgctcg atgccgtgat gcgtgacgcg gatgtgctcg tggcacgtgc cctgccacag
    26881 cagcttggcg cgaaagatgt gcaggttgcc cttggccagg ggctcgcgcc cgctgcgcgc
    26941 cggcaggtcg ggattcatca ggtcgatcac cagcaggctg ttggattcct ccaccgatga
    27001 gcccaggtgc agcgggcgcg cgccgtcgat ctggaactcc tggtgcgaga ggaatcgcgt
    27061 gtcgttgtgg tacaggccct cctttcccgg cgccagcacc tggatgtcgc caaagcggtc
    27121 gaacatggcg aaggtctggt cgttcttcag gacctggggc tgctcctcgg cactggccga
    27181 agtcgcggcc acgtaccact tgtcgccgat ctggattttc tgcggcattc gctttccttc
    27241 ttcttctccg ggatctaagc cgggatgcgt gcatccacca gctggcggta aacgtccagg
    27301 taatgccggg ccatgacccg cgccgcgaaa cgctgctcga acacctgccg gcagcggccc
    27361 cggtcgatgc ggtcgatgtc cttcgcggcg gcgatagcct gctcctggtt cgaaacgatg
    27421 aacccggtca ccccatgatc gatgatttcg ggtaccgacc cgtggcggta ggcgatcacc
    27481 ggcgtgccgc aagccattgc ctcgatcatg accaggccga agggctctgg ccagtcgatg
    27541 ggcatcagca gcgccctcgc gttgccgatg aaatcgttct tggctttctc gccgatttcg
    27601 cccacgaagg tcaccagcgg gtcgtccagc agcggctcga tgaccgactc gaagtagtcg
    27661 cggtcggccg ggtccacctt ggccgcgatc cgcagcggcg tgccgcaggc cctggcgatc
    27721 tcgatcgccc ggtcggcgcg cttttccgga gagatgcgcc cgacgaaggc gaagtagtcc
    27781 tgcggccgag cgcgcaacga gtacagcccc agcggaagtc cgtggtgcac ggtggcgcgc
    27841 cagttggccc atggcaccgg cgtgcgctgg ctgttggaga cggacaccat cgggtgattc
    27901 ccgaagcggc ggaacagcgg cttgatgtcg ggaaggtcga ggcggccatg cggcgtggac
    27961 aggcaggcgg tttcgcactg gcccaccagg gcgagctgca ggatgtcgat atggaaatga
    28021 atgatgtcga aggtgggcgc cgccttgagc accatgtcca tcatgatggt gtgccatacc
    28081 agcgggtccg gcttgtgcgg gtccagccgg agaccccagg gcaccaccgg caccagccgg
    28141 gccgaggtga ccgagtctcc gctggcgaac agcgtgacct catggcccat ctccaccagc
    28201 gtctcggtca gataggaaac aacgcgttcg gtgccgccgt aggcgcgcgg cggcacgctt
    28261 tcatacaaag gcgcgacctg ggcaattttc acgtggattc cactccttct gcggcggtcg
    28321 agctgcgcga gggcactcgg tcgggctggt tggaatgctg cgcagcactg accgctgccc
    28381 tgaacttatc tgtcagtgtt aaccctgagt gtaggacaac cccgcgtaat atcttcagcg
    28441 cgtccggcgc cgcgcactcg tcaggtaaac agctgtctgt gctgctcgcg caggagattt
    28501 ttctggacct tgcccatgct gttgcgcgga agctcatcga ccacgaaaca gcgcttgggg
    28561 atcttgaaac tcgccagctg cagtttgagt ttcgcgacga tatgctcggc atcgagctgc
    28621 acgcccggct tgggaatgac gaccgcgaca ccgacttcac cgaaatccgg atgcggcaca
    28681 cccaccagcg cgctctcggc cacgcccggc atttcgttga tgtaactctc gatctcggcc
    28741 ggatacacgt tgtagccgcc gctgatgatc agatccttgc tgcgcccgac gatggtgacg
    28801 tagcctcgtt cgtcgacctt gcccacgtcg ccggtcctga accagccgtc ttccgtgaac
    28861 tcttcctttg tcttgccggg catgcgccag tagcccttga agacgttggg tcccgtcacc
    28921 tcgatattcc cgatctcgcc ggcggccagt tcgcggccgt cgtcgccgcg cacgcgcagg
    28981 cccacgccgg gcaagggaaa gccgaccgtg ccgccgcgcc gctcgccgtc cccggcgtcg
    29041 aaggggttgg aggtcagcat gatggtttcg ctcatgccgt agcgctccag gatggtgtgc
    29101 ccggtgcgtt ccttccattc gttgaaggtt tcgagcagca gcggcgccga gcccgagatg
    29161 aacaggcgca tgtgccgtgc cgcggccggg tccaggccgc gctccgccag catccgcacg
    29221 tacagcgtgg gaacccccat gaacatggtg gcctcgctca tcttgcggat cacgagggcc
    29281 gggtcgaatt tgccgagcca gatcatcttg ctgccattga gcagggcgcc atggatcgcg
    29341 acgaacaggc catgcacatg gaagatcggc agcgcgtgga tcagaacgtc gccacccttt
    29401 tcggggcttc tccagcccca gtagtccttc agcaccttcg cgttggacag caggttgccg
    29461 tgcgtgagca tcgcgccctt gctgcggccg gtggtgccgc tggtgtagac gatcgcggcg
    29521 acgtcgtctt cgcccttggc cgcgatctca tggcggtcgc cgcaatgcgc cgcacgatcg
    29581 agcagcgtgc ccgttctatc ctcgttgagc gtgaagacgt ggcggctgtc cgtcttgaac
    29641 gcgatcttgc tgacccagcc gaagttgagt gcgccgcaca cgaccacggc gggctcggcg
    29701 ttgccgagga agtactcgat ctcggcgctg cggtacgcgg tgttgagcgg cagaaacacg
    29761 tagcccgcgc gcagcgtcgc caggtacagc atcatggcct cgaccgattt ctcgacctgc
    29821 gcggcgatgc gcgctccttc gggaagcttc agcgccagca gcaggttggc gatcatggcc
    29881 gtcgcgcggt cgaggtcgcg ccacgaatag accaggccgt ggtcggtctc cacggcctgg
    29941 gtatcgagat ccgccggaaa agccgcgcgc agcgcggcga agacattctg gttgttcatg
    30001 ttcagaaccc gggcttgcgt ttctcgagga aggcaccgat gccttcacgg tgttcggcgc
    30061 tgtcggcgta gcggtaggcg ccggcgtttg gctgcgtgcc ggcctgatcc gacgcttcgg
    30121 ctggccacgg ccgcggcgcc agcgcgcgca gcgcctgctt gttcaggcgc gcggcctgcg
    30181 gcgccagcgt tgccacgcgt tgcgcgctcg cccaggcctg cgcccccact tcggcgtcgg
    30241 ccgccacccg gttcaggaag ccccggtttt tcatctccac ggccggcagc acggccgctt
    30301 ccaacagcat ctcgcgcacg gtcgactcgc ccgccgcgcg caggaccagt tgcgcctcgc
    30361 gcggcgccat cgggaagccc agcttcgcga tgggcgcgcc gaacgcggcc gattcggcgg
    30421 cgacacgaat atcgcagcag ctcgcgattt ccagtccggc gcccatgcag gcaccctcga
    30481 tccgcgcgac cagcggcacg tcgcacgcca gcatggcggc caggccgccc cagacatcgc
    30541 tctcgtggaa gtcgcgcagc gcggcttcgc tgaagcggaa cccggggtat tccgagatgt
    30601 cgccccccgc gcagaacgtg ccgccctccc cctgcaccac cacgcagcgc cactggctat
    30661 ccgactggat cctctcgaac accgcgcgca gctcgcgcca catcgcgcgc gacatggcgt
    30721 tgagcttgcc cggatgggac aaggtgacga cggcgatggt gccttcgctg gtgaaaagaa
    30781 cctgtccggg catcgctagg gcctggccgg ccaggtgggc tgcgcgcggg cgagcgcggt
    30841 cagcatgccg gcgccggtcg cggcggcgcg caaaacgtct cgaatgctaa ggctgtggtt
    30901 caccgtcttt gtctccttgt cgtgaatgaa ttcaaggcaa tcgccgcacc acccggcggg
    30961 cagccggcaa taggcctgcc gctgccgaaa tctcaaaagt acaggtcttc gatcgcccca
    31021 gaaatcggga ttt
//
LOCUS       DASWIY010000491         6422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2947218, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000491 DASWIY010000000
VERSION     DASWIY010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2947218"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VGA59_17700"
     CDS             complement(<1..174)
                     /locus_tag="VGA59_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEX9721555.1"
                     /translation="MAEFKEISPNAALGEKLANWFDNRFPSAGSMYRDHMSEYYAPKN
                     FNFWYFFGSLAMLV"
     gene            complement(241..837)
                     /gene="petA"
                     /locus_tag="VGA59_17705"
     CDS             complement(241..837)
                     /gene="petA"
                     /locus_tag="VGA59_17705"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX9721556.1"
                     /translation="MSDTTVDASKRTWLIASGCAGVVGGAFTAVPFVSSFQPSEKARA
                     AGAPVEVDIAALKPGEKITVEWRGKPVWILKRTPEQLQTLKKTSSEVADPNSERKAYP
                     TPEYARNEYRSIKPEVWVGVGICTHLGCSPSDKLQAGPQASMPDDWLGGFFCPCHGST
                     FDLAGRVFKNKPAPDNLEVPPHMYLSDSRLLIGEDTKG"
     gene            971..1399
                     /gene="mscL"
                     /locus_tag="VGA59_17710"
     CDS             971..1399
                     /gene="mscL"
                     /locus_tag="VGA59_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX9721557.1"
                     /translation="MAMLREFREFALKGNVVDLAVGVIIGAAFGKIIDSVVGDLIMPL
                     VGALIGKLDFSNLFVVLGQVPAETPATLDALKKAGVPVLAYGSFITIAVNFLLLALVV
                     FLMVRQINRLRRENPPPAPPPAPPEDIVLLREIRDSLRRN"
     gene            complement(1396..2448)
                     /gene="pdxA"
                     /locus_tag="VGA59_17715"
     CDS             complement(1396..2448)
                     /gene="pdxA"
                     /locus_tag="VGA59_17715"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX9721558.1"
                     /translation="MSVAQGLQPVAITMGDAAGIGPEIVAKAFRDEPQVTRGCFVAGD
                     LASMRRATRVIDAGRGVELPIALIEAPAAALAAPPRCIPVLEAARLARPPEWGRVSAA
                     AGKAAAECVVWAARAALGGEIAALVTAPLHKEALAAAGVAYPGHTELLQAEAAAWLGK
                     DLADLPVRMMLANDELRTVLVSIHVSLRDALAAVNFDNVLQTLRITHHAVRRSLGRSP
                     HIGVAGLNPHAGEGGLFGREEIDSIIPAIEAARAEGLHVSGPFAPDTVFMRARAVGGR
                     PGEFDVVLAMYHDQGLIPVKYLGVDKGVNVTLGLPLVRTSPDHGTAFDIAGTGRADAS
                     SLVEAIRVARRLSEAG"
     gene            complement(2480..3238)
                     /locus_tag="VGA59_17720"
     CDS             complement(2480..3238)
                     /locus_tag="VGA59_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX9721559.1"
                     /translation="MATAQQFLQAFDGLLQPERFKDYGPNGLQVEGKGEVGRIVSGVT
                     ASRALIEAAIEARADAIFVHHGLFWRGHDGRVTGWLKQRLALLLKHDVNLFAYHLPLD
                     AHPELGNNAQLGLQLDLEATARFGEQDLGFLGLRRDGGSFAGARELASHIERRLGRPA
                     TWVEAQQRTIRNIAWCTGGAQGYFEPAIAAGADAFITGEISEPQVHYARECGVAFLAC
                     GHHASERYGAPAVAGHVAAQLGVEHRFIEIDSPA"
     gene            3394..4653
                     /locus_tag="VGA59_17725"
     CDS             3394..4653
                     /locus_tag="VGA59_17725"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX9721560.1"
                     /translation="MNAQELTIWSMALGAIGAMTMARLADLMVRPSLAQVQGVAYHVT
                     VFMLVLILSGVIVQLWPQIEPAWVRGSQVLAGPVCVGLSNFWIRGWLNAAQRDRVMAS
                     ALHASAVLLPLGGLACLALPEGQQIPAAGAISLLGAGMTLWLTVRAWLMGDRLAPVMA
                     AGCLLTLPAIAGLHALALRLPGFGVPLQGAAALSAALSNGLTGFVLWRRDRHEWRTRR
                     EDGQPLQFDPVTKLYSGISLVRKLVKAQRRRRRTRRDGAVLAILVFDVDRVAAQAGTA
                     GVNEMFICIASRIQRQVGVINPVGRYYDSCFVTLVETIQSPAWLRTLGLRVASSLRRP
                     IEVMTASGQRIELKADVGVGVVHLAQGPAAVEDILHDAQRMAEAARGMRSRAAILDPY
                     SGEVVPVEHANLGPRRHGHAHLVPHAL"
     gene            4767..5918
                     /locus_tag="VGA59_17730"
     CDS             4767..5918
                     /locus_tag="VGA59_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9721561.1"
                     /translation="MRRFWLLFSQAVTVLLAAYFVVATLKPEWLDRPRMTVTSQGVPL
                     LEAPAPVAGPPPAGSLRQAAQRASAAVVSINTSKAVRHPGANDPWFRFFFGDQGGGMP
                     QAGLGSGVIISADGYILTNNHVIEGADEIEVVLNDSRRTPAKVIGTDPDTDLAILRVS
                     LERLPAVTLGNSDALQVGDQVLAIGNPFGVGQTVTSGIVSALGRNQLGINTFENFIQT
                     DAAINPGNSGGALVDVNGHLMGINTAIYSRSGGNMGIGFAIPVSTAKLVLEGIVKEGV
                     VRRGWIGVEPADLSPELMQTFGVKAKRGVLITGVLQNGPAAQAGIRPGDVIVEVAGKQ
                     IANVSELLSNVAGLKPGAPAKFRVLRHDDAVELNVTPGLRPRPPKLPQR"
     gene            complement(5937..>6422)
                     /gene="tatC"
                     /locus_tag="VGA59_17735"
     CDS             complement(5937..>6422)
                     /gene="tatC"
                     /locus_tag="VGA59_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX9721562.1"
                     /translation="WAFVAPGLYSHEKKLVVPLVVSSTLLFFVGVGFCYFFVFGQVFS
                     FIQSFAPKSITAAPDIEAYLSFVLTMFIAFGLAFEVPIVVVVLARMGFVSIEKLKSFR
                     SYFIVLAFIIAAIITPPDVVSQLALAIPMCLLYEAGIWAAQLFIKHTKAPEEGAAAAD
                     S"
BASE COUNT         1060 a         2180 c         2126 g         1056 t
ORIGIN      
        1 caccagcatg gccagcgagc cgaagaaata ccagaagttg aagttcttcg gcgcgtagta
       61 ctccgacatg tggtcccggt acatggaccc cgccgaagga aagcggttgt cgaaccagtt
      121 ggcgagcttt tcgcccaggg cggcgttggg agaaatttcc ttgaattcag ccatgaatcg
      181 tcttgatcgg ttggggacgg gagcgcaccg cgggcaatcc tgatccgtcc cgcaagaatc
      241 tcatcctttc gtgtcttcgc cgatgagcag acggctgtcc gagaggtaca tatgcggcgg
      301 cacttccagg ttgtccggcg cgggcttgtt cttgaacacg cggcccgcga gatcgaaggt
      361 cgagccgtgg caggggcaga agaagcctcc cagccagtcg tccggcatgg acgcctgggg
      421 accggcctgc aacttgtccg aaggcgaaca gcccaggtgc gtgcagatgc ccacgcccac
      481 ccagacttcg ggcttgatcg agcggtattc gttgcgggcg tactccggcg tggggtaggc
      541 tttgcgctcg gaattgggat cggccacctc actggaggtt ttcttcagcg tctgcagctg
      601 ctcaggcgtg cgcttgagga tccataccgg cttgccgcgc cactccaccg tgatcttctc
      661 gcccggcttg agcgctgcaa tgtcgacctc cacgggcgcc ccggccgccc tggccttttc
      721 ggagggctgg aagctgctca cgaagggaac ggccgtaaac gcgccgccca ccacgccggc
      781 acaaccggac gcgatcagcc acgtgcgttt gctggcgtcc actgtggtgt cactcatcgg
      841 gggtcctcaa gggtgtcgct tgattagggt caacccgcga ttgtagccga cggtgtatga
      901 cagactcaaa ggcctgccac cgctgcgcga acgggctcat actccggccg atcaacaacg
      961 gaggccggcc atggcaatgc tgcgggagtt cagggagttc gcactaaagg gcaacgtggt
     1021 ggatctcgcc gtaggcgtca tcatcggcgc cgcgttcggg aagatcatcg attcggtcgt
     1081 gggcgacctg atcatgccgc tggtgggcgc gctcatcggc aagctggact tttccaatct
     1141 cttcgtcgtg ctgggccaag tgcccgcgga gacgcccgcg acgctggatg ccctgaagaa
     1201 agccggcgtg ccggtcctgg cctacggcag cttcatcacg atcgccgtca atttcctgtt
     1261 gctcgccctc gtcgttttcc tcatggtcag gcagatcaac cggctgcggc gcgagaaccc
     1321 gccgccggca cccccgccgg cccctccgga ggacatcgtt ctgctccgcg agatccgcga
     1381 cagcctgcgc cggaactagc cggcttcgct cagccggcgc gcgacgcgaa tcgcctcgac
     1441 gaggctggac gcgtcggcgc ggccggtgcc cgcgatatcg aacgccgtgc cgtgatcggg
     1501 gctggtgcgc accagcggca gacccaatgt gacgttgacg cccttgtcga cgcccaggta
     1561 cttcacgggg atcagtccct ggtcgtggta catcgcgagc accacgtcga actccccggg
     1621 acggccgccc accgcgcgag cgcgcatgaa gaccgtgtcc ggtgcaaaag gaccgctcac
     1681 gtgcaggccc tcggcgcggg ccgcctcgat cgcgggaatg atggaatcga tttcctcgcg
     1741 cccgaacagc ccgccttcgc cggcgtgcgg gttgagcccc gccacgccga tgtgcggcga
     1801 gcggcccagc gaccgccgca ccgcatggtg cgtgatgcgc aaggtttgca ggacattgtc
     1861 gaagttcacg gccgcgagcg catcgcgcag cgaaacatga atgctcacca gcaccgttcg
     1921 caactcgtca ttggccaaca tcatgcggac cggaaggtcg gccaggtcct tgcccagcca
     1981 agccgccgcc tcggcctgca gcagctccgt gtggccgggg tacgccacgc cggccgccgc
     2041 cagcgcctcc ttgtgcaacg gcgccgtcac gagcgcggcg atttcgccac ccagcgcggc
     2101 ccgcgccgcc cacaccacgc actcggccgc ggccttcccg gcagcggcgc tgacccggcc
     2161 ccattccggc ggccgggcca ggcgagccgc ttccagcacg gggatgcagc gtggcggagc
     2221 cgccagcgcc gcggccggcg cttcgatcag cgcgatcgga agctccacgc cccgcccagc
     2281 gtcgatgact cgcgtggcgc ggcgcatgct ggccaggtcg cccgccacga agcagccgcg
     2341 cgtgacctgc ggctcatccc tgaaggcttt ggcgacgatt tcggggccga tgcccgccgc
     2401 atcgcccatg gtgatggcga ccggttgcaa gccttgcgca acgctcaccg ggttacctcg
     2461 tctttcaggg tcggacggat catgcggggc tgtcgatctc gatgaagcgg tgctcgacgc
     2521 ccagctgcgc cgccacatgg ccggcaacgg caggcgcgcc atagcgctcg ctggcatgat
     2581 ggccgcaggc gaggaaagcg accccgcatt cgcgcgcgta atgcacttgc ggctcggaaa
     2641 tctcccccgt aatgaaggca tccgctccgg ccgcgatagc gggttcgaag tacccctggg
     2701 caccgccggt gcaccatgcg atgttccgga tcgtccgttg ctgtgcctcg acccaggtcg
     2761 ccggccggcc caggcgccgt tcgatgtgcg acgccagttc ccgcgcgccg gcgaagctgc
     2821 cgccgtcacg gcgcagtccg agaaacccga gatcctgctc accgaaccgc gcggtcgcct
     2881 ccagatccag ctgcagtccc aactgggcgt tgttccccag ctcgggatgg gcgtccagcg
     2941 gcaggtgata ggcgaacagg ttcacatcgt gcttgagcag cagcgccagc cgctgcttca
     3001 gccaaccggt gacccggcca tcgtggccgc gccagaaaag gccgtggtgc acgaagatcg
     3061 cgtcggcccg cgcctcgatc gccgcctcga tcagagcgcg gctggcggtg acgcccgaga
     3121 caatgcgccc cacctcgccc ttgccctcca cctgcagccc gttgggcccg tagtccttga
     3181 agcgttcggg ctggagcaag ccgtcgaaag cctgcaggaa ctgttgcgcg gtggccatgg
     3241 ggaatcgtcg tactccaaaa caccgattgt cggccaaccg cgccggcacc cgcgcgtccc
     3301 gatgtccgct gctcaaggca catagggtcc tccccatggc ttgcagctgc cgagctcgca
     3361 ataatacatt cgttcacagg gcgggagggg ctcatgaatg cgcaggagtt gacgatctgg
     3421 agcatggcac tgggtgccat tggggcgatg accatggccc gactggcgga cctgatggtg
     3481 cggccgagct tggcgcaagt ccagggcgtc gcttatcacg tcaccgtatt catgctggta
     3541 ctgatcctca gcggagtgat cgtgcagctg tggccgcaga tcgagccggc ttgggtgcgg
     3601 ggctcgcaag tgctggccgg cccggtgtgc gtggggcttt ccaatttctg gatccgcggc
     3661 tggctcaatg ccgcgcagcg cgaccgcgtc atggcttcgg ccttgcatgc gtcggccgtg
     3721 ttgctgccgc tcgggggact ggcatgcctg gccttgcccg agggacagca gattcccgcc
     3781 gccggcgcca tttcgctgct cggcgccggc atgaccctgt ggttgacggt gcgcgcctgg
     3841 ctcatgggcg accggctcgc cccggtcatg gcggcgggct gcctgctgac gttgccggcc
     3901 atcgccggcc tgcacgccct cgcgttgcgc ctgccgggat tcggcgtgcc gctgcagggc
     3961 gcggcggccc tgtccgcggc cctgtcgaac ggcctgaccg gtttcgtgct gtggcgccgt
     4021 gaccggcacg agtggcgcac ccggcgcgaa gacggccagc cactgcagtt tgaccctgtc
     4081 accaagctct acagcggcat cagcctggtg cgaaaactgg tcaaggccca gcgccggcgc
     4141 cgccgcaccc gccgcgatgg tgcggtcctg gcgatcctgg tgttcgatgt cgaccgggtc
     4201 gcggcgcagg ccggcaccgc cggcgtgaac gagatgttca tctgcatcgc cagccgcatc
     4261 cagcgccagg tgggcgtgat caatccggtg ggccggtatt acgacagctg cttcgtgact
     4321 ctggtggaaa ccatccagtc gccggcctgg ctgcgcacgc tggggctgcg ggtcgcaagc
     4381 agcttgcgcc ggccgatcga ggtcatgact gcgtcgggcc agcgcatcga gctcaaggcg
     4441 gacgtgggtg tcggcgtggt ccatctcgcg caagggcccg ccgccgtcga ggacatattg
     4501 cacgacgccc agcggatggc ggaggcggcg cgcggcatgc gctcgcgcgc cgcgatcctc
     4561 gatccctatt ccggggaagt ggtgccggtc gagcatgcca acctcgggcc gcgccgccat
     4621 ggccatgccc atctggtccc gcacgcgcta taggtagctg gcggcgtgcg ctgaattttt
     4681 cgcctgcagc ggcagcgccg gccgcatagg cggccgcgcg cgtctacact tgcgagaaaa
     4741 ctactttcgg ggttcccgaa tcgatcatgc gtcgcttctg gctgcttttt tcccaggctg
     4801 tcaccgtcct gctcgcggcc tatttcgtcg tggcgacgct caagcccgaa tggctggacc
     4861 gcccccgcat gaccgtcacc agccagggcg ttcccctgct cgaagcgccg gcgccggtag
     4921 ccggcccccc tcccgccggc agcttgcggc aggcggccca acgcgcctcg gccgccgtgg
     4981 tcagcatcaa caccagcaag gccgtgcgtc accccggcgc caacgatccc tggttccgct
     5041 tcttcttcgg cgaccagggg ggcggcatgc cgcaagccgg gctgggcagc ggcgtgatca
     5101 tcagcgccga cggctacatc ctgaccaaca accacgtgat cgagggcgcc gacgagatcg
     5161 aggtggtgct caacgacagc cgcagaacgc cggccaaggt gattggcacc gaccccgaca
     5221 cggacctggc gatcctgagg gtgtcgctgg agcggctgcc ggcggtcacc ttgggcaact
     5281 ccgacgcgct gcaggtgggc gaccaggtgc tggcgatcgg caatccgttc ggcgtgggac
     5341 agacggtcac cagcggtatc gtcagcgcgc tcgggcgcaa ccagctgggc atcaatacct
     5401 tcgagaactt catccagacc gacgccgcca tcaaccccgg caattccggc ggagcgctgg
     5461 tggacgtgaa tggccacctg atggggatca atacggcgat ctattcccgc tcgggcggca
     5521 acatgggcat cggcttcgcg atccccgtct ccaccgccaa gctggtgctc gaaggcatcg
     5581 tcaaggaagg cgtcgtcagg cgcggctgga tcggcgtcga accggccgac ttgtcgcccg
     5641 aattgatgca gacattcggc gtcaaggcca aacggggcgt gctcatcacc ggcgtgctgc
     5701 agaacggtcc ggccgcgcaa gccggtatcc gtcccggcga cgtcatcgtg gaagtggcgg
     5761 gcaagcagat tgccaacgtc tcggagctgc tatccaatgt ggccggcctc aagcccggag
     5821 cacccgccaa attccgcgtg ctgcggcacg acgatgcggt ggagctgaat gtcacgccgg
     5881 ggttgcggcc gaggccgccg aagctgccgc agcgataagc ccaggacccc acccgatcag
     5941 gaatcagcgg ccgcggcgcc ctcctcgggc gccttggtgt gcttgatgaa caattgcgcg
     6001 gcccagatgc ccgcttcgta cagcaggcac atcgggatgg ccagggccag ctgggacacc
     6061 acgtcgggcg gcgtgatgat ggccgcgatg atgaacgcca gcacgatgaa gtacgagcgg
     6121 aacgacttga gcttctcgat gctgacgaag cccatgcgcg ccagcacgac caccacgatg
     6181 ggaacctcga acgcgagtcc gaaggcgatg aacatcgtca gcacgaaact caggtaggcc
     6241 tcgatgtccg gcgccgcggt gatgctcttt ggcgcgaagc tctggatgaa cgagaacacc
     6301 tgcccgaaca cgaagaagta acagaacccc accccgacga aaaacagcag ggtgctggac
     6361 acgaccaggg gcacaaccag ctttttttcg tgcgagtaca gccctggagc gacgaaagcc
     6421 ca
//
LOCUS       DASWIY010000492         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1467505, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000492 DASWIY010000000
VERSION     DASWIY010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467505"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..445
                     /locus_tag="VGA59_17740"
     CDS             107..445
                     /locus_tag="VGA59_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX9721563.1"
                     /translation="MKMLMAIIKPFKLDAVREALAEIGVQGMTVTEVRGFGRQKGHTE
                     LYRGAEYTVDLLPKIKLELALTPEQVDKAIEAIQKSAKTGQIGDGKIFVYDLNETVRI
                     RTGETGKEAI"
     gene            545..1828
                     /locus_tag="VGA59_17745"
     CDS             545..1828
                     /locus_tag="VGA59_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX9721564.1"
                     /translation="MLKLICAVVSVLLPVSAHAQELSAGDTAWMITATALVLFMTLPG
                     LALFYGGLVRTKNVLSVLMQCFVIACVASVIWVIYGYSLAFAPGGPLIGDLSKAFFNG
                     VTESSLSGTIPETVFAMFQLTFAIITPALVVGAFAERMKFSAMVLFSALWLTLVYLPV
                     CHWVWAGDGWLFKLGLLDFAGGTVVHTTCGVAALVAALVLGKRSGFPTAPMMPHNMTM
                     TAMGAGMLWVGWFGFNAGSALAANGNAGMAMLATHVSAAAGALAWMVLEWLRYGRPSV
                     LGVVTGIVAGLATITPASGFVGPGGALVIGLLAGAVCFTATSYMKRVLKVDDSLDVFP
                     VHGIGGMLGTVLAGVFASTGLGIFSGQGYAEGMTMGSQLGVQLTGVAAIAAYTGAVTF
                     VLLKAVDRVAGLRVSSENETLGLDQAEHNERGYNL"
     gene            1927..2295
                     /locus_tag="VGA59_17750"
     CDS             1927..2295
                     /locus_tag="VGA59_17750"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX9721565.1"
                     /translation="MRLMIVSLLTLACAVAQATEVYKWKDKDGRVHYGDKPKHEAQSV
                     DIGPADPVGPLTSDEEATMVARKAECDRMKQQYDVYSKAPSINETDNLGRTREYTPEE
                     RTKFLEQTQQKMQDACAPAQ"
     gene            complement(2317..>2635)
                     /locus_tag="VGA59_17755"
     CDS             complement(2317..>2635)
                     /locus_tag="VGA59_17755"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721566.1"
                     /translation="LRKDGLLVQQSESPLLHLGLIREMHQAMRQAGFAQTHLIHFPQP
                     IYPSGWWSASFARKTRGKLAERLSPKAVAGLKTQYYNAATHRAAFAVPSFVGAALAAT
                     GRA"
BASE COUNT          428 a          870 c          891 g          446 t
ORIGIN      
        1 cccattggcg ttccatattt gtttgcgcgt ccgaaaatcc ccttttgcac catttttggg
       61 cggcgtttag actcccccgg ccgttcgctc aacccgggga cccagaatga aaatgctgat
      121 ggcgatcatc aagccgttca agctcgacgc cgtgcgcgaa gcgctggccg aaatcggcgt
      181 gcagggcatg accgtcaccg aggtgcgcgg cttcggccgc cagaaggggc acaccgagtt
      241 gtaccgcggc gccgagtaca cggtggacct gctgcccaag atcaagctgg aactggcgct
      301 gacgcccgag caggtcgaca aggccatcga ggcgatccag aagtccgcca agaccggcca
      361 gatcggcgac ggcaagattt tcgtctacga cctcaacgag accgtccgca tccgaaccgg
      421 cgagaccggc aaagaggcga tctagaattt ccgtcatgcc cgcgcaggcg ggcatccaat
      481 gctcgtccaa aaactggatt cccgcctgcg cgggaatgac gaccaccaga gtcgcgggga
      541 gaccatgctc aaactgatat gtgctgttgt ctctgttctg ctgcccgttt cggcccacgc
      601 ccaggagctc agcgccggcg acaccgcctg gatgatcacg gccaccgcgc tggtgctgtt
      661 catgacgctg cccggcctgg ccctgttcta cggcgggctg gtgcggacca agaacgtgct
      721 gtccgtgctg atgcagtgct tcgtcattgc ctgcgtggcg tcggtgatct gggtcatcta
      781 tggctacagc ctggcctttg cacccggcgg ccccctgatc ggcgacctgt ccaaggcgtt
      841 cttcaacggc gtcaccgaaa gctcgctgtc cggcacgatt cccgaaacgg tgttcgcgat
      901 gttccagctg accttcgcga tcatcacgcc ggccctcgtg gtcggcgcgt tcgccgaacg
      961 catgaagttc tcggcgatgg tgctgttctc ggcgctgtgg ctcaccctgg tctacctgcc
     1021 ggtctgccac tgggtctggg ccggcgacgg ctggctgttc aagctcggcc tgctcgattt
     1081 cgccggtggc acggtcgtgc acaccacctg cggcgtcgcc gcgctggtgg cggcgctggt
     1141 gctcggcaag cgcagcggct ttccgaccgc gccgatgatg ccgcacaaca tgacgatgac
     1201 cgcgatgggc gccggcatgc tgtgggtcgg ctggttcggc ttcaacgcgg gcagcgcgct
     1261 ggcggccaac ggcaacgccg gcatggcgat gctggcgacg cacgtctcgg ccgcggccgg
     1321 cgcgctggcc tggatggtgc tcgaatggct gcgctacggc cgccccagcg tgctcggcgt
     1381 ggtcaccggc atcgtggccg gtctcgccac catcacgccc gcctcgggct tcgtcggtcc
     1441 cggcggggcg ctggtgatcg gcctgctggc gggcgcggtc tgcttcaccg cgaccagcta
     1501 catgaagcgc gtactcaagg tcgacgattc gctggacgta tttcccgtgc acggcatcgg
     1561 cggcatgctc gggaccgtgc tggcaggcgt gttcgcgagc accggcctcg gcatcttcag
     1621 cggccagggc tatgccgagg gcatgaccat gggcagccag ctcggcgtgc agctgaccgg
     1681 cgtggccgcg atcgccgcgt acaccggcgc cgtcactttc gtgctgctga aggcggtgga
     1741 cagggtcgcg gggctgaggg tgtcgtccga gaacgagacg ctggggctgg accaggccga
     1801 gcacaacgag cggggttaca acctgtagct ggcgctggcg gtcgggggcc cgcgggctcc
     1861 catcggcgcg cgtattgacc gcgcccggcg cttctcggag actggttcgc cattggaggg
     1921 agccccatgc gcctgatgat cgtctcgctg ctgaccctgg cctgcgccgt tgcccaggcc
     1981 accgaggtct acaagtggaa ggacaaggac ggccgcgtcc actacggcga caagcccaag
     2041 cacgaggcgc agtcggtcga catcgggccg gccgatccgg tcgggccgct gaccagcgac
     2101 gaagaagcca cgatggtggc gcgcaaggcc gagtgcgacc gcatgaagca gcagtacgac
     2161 gtctacagca aggcgccttc gatcaacgag accgataacc tcggccgcac gcgcgaatac
     2221 acgcccgagg agcgcaccaa gttcctcgag cagacgcagc agaagatgca ggacgcctgc
     2281 gcgcccgcac agtaacccgt ccgtgtagga gcgccttcag gcgcgaccgg tcgcggctaa
     2341 agccgctcct acaaaagatg gcaccgcgaa tgccgcgcgg tgcgttgcgg cgttgtagta
     2401 ctgcgtcttc aggcccgcga ccgccttcgg tgacagccgc tcggccagct tgccgcgggt
     2461 cttgcgcgcg aacgaggccg accaccagcc cgacgggtag atcggctgcg gaaagtggat
     2521 caggtgggtc tgcgcgaagc cggcctggcg catggcctgg tgcatctcgc ggatcaggcc
     2581 caggtgcagc agcggcgatt ccgattgctg caccagcagg ccgtccttgc gcagg
//
LOCUS       DASWIY010000493         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2577459, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000493 DASWIY010000000
VERSION     DASWIY010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577459"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VGA59_17760"
     CDS             complement(<1..371)
                     /locus_tag="VGA59_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX9721567.1"
                     /translation="MTARSFFRYAVTLLMLLSGAVVAQEPGGREILDRVERLLWGTRV
                     QGDYEMTISTPRWQRTLGLRLWMERPKRSFVRIVSPAKEAGIGSLRLGSEMWNYLPNV
                     ERIVRIPPSMMLQPWMGSDFT"
     gene            complement(374..667)
                     /locus_tag="VGA59_17765"
     CDS             complement(374..667)
                     /locus_tag="VGA59_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721568.1"
                     /translation="MPHAKLSRTKTGMDPTQGPAVEKAVVQPRSRIKVSIHQLDDAGT
                     RTGAPSPQELIEYKSDFYERMILSLLLRQPGLFEKAMRGVTRTGINTGQNSRG"
     gene            complement(762..1052)
                     /locus_tag="VGA59_17770"
     CDS             complement(762..1052)
                     /locus_tag="VGA59_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721569.1"
                     /translation="MTMAHSLHVAERRHSGTPLGLMQAPRVKPPSSLKAASHQPKSDA
                     IEALAPSLQELVEFQREFYERTIPLWNAMLKRMGTSEMDTSAPSSKRAGKEE"
     gene            complement(1327..>2250)
                     /locus_tag="VGA59_17775"
     CDS             complement(1327..>2250)
                     /locus_tag="VGA59_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX9721570.1"
                     /translation="GRPAFERAVASDGFDDTEVLRLAASLDQGSEHPLAAAIVAAARE
                     QGLTLSKPEQFDSESGIGVRGSVDGKALALGNTALMDQLGVGVAALTTQAEELRSQGA
                     SVMHLAVDGKLAGLLAVSDPIKATTLDALTMLRKSGLRIVMATGDGLTTARAVGKRLG
                     IDEVLGEVKPADKLKLVERLQSEGRVVAMAGDGINDAPALARADVGIAMGTGTDVAMN
                     SAQLTLVKGDLRGIAAARALSVATVANMKQNLGFAFIYNSLGVPLAAGVLFPFTGLLL
                     SPLIAALAMSLSSVSVITNALRLRGAKVRQD"
BASE COUNT          392 a          748 c          683 g          427 t
ORIGIN      
        1 ttggtgaagt ccgagcccat ccagggctgc agcatcatcg agggcgggat cctgacgatg
       61 cgctccacat tgggcagata gttccacatt tcggaaccca gccgcaagga accgatgccc
      121 gcttccttgg ccggcgacac gatacgcacg aacgaacgct tggggcgctc catccagagc
      181 cgaagaccga gcgtgcgttg ccagcgcggc gtgctgatcg tcatctcgta gtcgccctgc
      241 accctcgtgc cccataaaag ccgctcgaca cggtcgagga tttcgcgccc gcccggctcc
      301 tgcgccacca ccgcgccgct cagtagcatg agcagcgtca cggcataccg gaaaaatgaa
      361 cgtgcagtca ttcctaacct cttgagttct gtccggtgtt gattccggtg cgggtcactc
      421 ctcgcatggc tttctcgaac aatccgggct gccgcaacag caggctgagg atcatgcgct
      481 cgtagaagtc gctcttgtac tcgatcagtt cctgcggaga aggcgccccg gtacgggtgc
      541 ctgcgtcatc cagctggtgg atcgatacct tgatgcggga ccgcggttgg acaacggcct
      601 tctcgactgc cggcccctgg gtagggtcca tgcccgtctt ggtgcgcgac aacttcgcgt
      661 gcggcatgcg atcgctgtcc cgtactgatg tcgttctcac gaactctttc gccatcgttt
      721 tcccaatttc agtcgatatt catggcttca aattcatggc ctcattcctc tttcccggca
      781 cgcttgctgg atggtgcgct cgtgtccatt tcagatgtcc ccatgcgctt gagcatggcg
      841 ttccacaatg ggatcgtgcg ttcatagaac tcgcgctgga actcaacaag ctcctgcagc
      901 gacggcgcca aggcttcgat ggcatcagat ttcggctggt ggctcgctgc cttcaagctg
      961 gacggcggtt tcacgcgagg cgcctgcatc agcccgagtg gcgttcccga gtgccgtcgc
     1021 tctgccacat gcaggctatg agccatggtc atgattcatc tcctcgaact gctgatcggc
     1081 tcagggtcgg gacggagcgg aggggccgag caggcctgga ccaaccccgc accggcccat
     1141 tcacgccagg ttttcggtgc aaggtatcga taaagcaccc aggacaccac cacgatcacg
     1201 atcgcggcaa ggccccaaac gcctgaatgt gaaattgcgt cgttcaaaat aggagtcctg
     1261 ttcaagttgg gtgatggagc gctgcgaaac gctggtcatc agcatcgaat tgatgtgcgt
     1321 ttccggtcag tcctgcctga ccttcgcccc gcgcaggcgc agggcgttgg tgatcaccga
     1381 caccgagctc aggctcatcg ccagcgccgc gatgagcggc gacagcaaca agccggtgaa
     1441 cggaaacagc acgccggcgg ctagcggcac gccgagggag ttgtagatga aggcgaagcc
     1501 caggttctgc ttcatgttgg ccaccgtggc caccgacagg gcgcgcgccg ccgcgatgcc
     1561 gcgcaggtct cccttgacca gcgtgagctg ggcgctgttc atcgccacat ccgtgccggt
     1621 gcccatcgcg atgccgacgt cggcgcgcgc cagcgcgggt gcatcgttga tgccatcgcc
     1681 ggccatcgcg accacgcggc cttccgactg caacctttcg accagcttca gcttgtcggc
     1741 gggcttgact tcgccaagca cctcatcgat gcccaggcgc ttgccgacgg cgcgggccgt
     1801 ggtcaggccg tcgccagtgg ccatcacgat gcgcaagccc gacttccgca acatggtcag
     1861 agcgtcgagt gtcgtggcct tgatcgggtc ggacaccgcg agcaagccag ccaactttcc
     1921 atcgactgcc agatgcatga cgctggcgcc ctggctgcgc agttcctcgg cctgggtggt
     1981 cagcgcggcg actcccacgc cgagctggtc catcagcgcg gtgttgccca gcgccagcgc
     2041 cttgccatcc acgctgccac gcacgccaat ccccgattcg gaatcgaact gctccggctt
     2101 cgacagggtc agcccctgct cgcgcgctgc cgcgacgatg gcggcagcca gcggatgctc
     2161 gctgccctgg tccaggctcg cggccagacg cagcacctcg gtgtcgtcga agccgtcgga
     2221 agcgacggca cgctcgaagg ccggccggcc
//
LOCUS       DASWIY010000494         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3318155, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000494 DASWIY010000000
VERSION     DASWIY010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3318155"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VGA59_17780"
     CDS             complement(<1..1423)
                     /locus_tag="VGA59_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX9721571.1"
                     /translation="MKLLHFAALATLLWLAGCATPPPSDPVAAVSTPAKPAEPAAPSV
                     IPGGPLKPITASDAASLAVMQLEPPADLWARIRRGFAMPNLDSDLVRQQEQWYVSRPD
                     YIQRMTERSRKYLFHIVEELERRNMPTELALLPFIESAFNPQAVSSARAAGMWQFMPA
                     TGSYFELKQNVFRDDRRDVLASTRAALDYLQKLHGMFGDWHLALAAYNWGEGSVGRAI
                     ARNQKSGLGTGYGDLTMPNETRYYVPKLQAVKNIVADPEALRAELPLIENHPYFQTVT
                     VSRDIDVELAARLADVSVADFKALNPSMKRPVILAAGTPEILLPWDNAKIFQRNFEAY
                     TDGQYASWTAWSAPATMSASDAARRVGMTEADLRTVNTIPPRMLIKAGSILIVPRSAK
                     MQNDVTSHVADNGQLNLSPERVTRRTWVKARKHETVASVARRYRLRPDQIAEWNRVGA
                     AAAFKAGQRVVVYLPVKTGVGGRA"
     gene            1866..2651
                     /locus_tag="VGA59_17785"
     CDS             1866..2651
                     /locus_tag="VGA59_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9721572.1"
                     /translation="MSDQIIGMQQWFETPPGRYLLAWERAEFDRAVGDIFGFHALQLG
                     LPELDTLQANRMPHKWLALREPPLEGSAQPKASLITDFAALPFEANSLDLVVLPHSLE
                     LNTDPHATLREVERVLVPEGKVVICCLNPASLWGLRQRRAHLYRRLGFGELYLPDAGD
                     FIGYWRLRDWLRLLSFEVESGNFGCYRPAMSTEKWLDRYDWLDHAGERWWPIFGAVYF
                     LVAVKRVRGIKLIGPAWKGSKAIAAKPVPLANRSHPAHLEEHV"
     gene            2648..>2811
                     /locus_tag="VGA59_17790"
     CDS             2648..>2811
                     /locus_tag="VGA59_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HEX9721573.1"
                     /translation="MNSVEIYTDGACKGNPGPGGWGVLLKSGGTEKELFGGERLTTNN
                     RMELMAVIQAL"
BASE COUNT          459 a          858 c          975 g          519 t
ORIGIN      
        1 gtgcccggcc acccactccg gtcttgaccg gaaggtagac gaccacccgc tggccggcct
       61 tgaacgcggc cgcggcgccg acgcggttcc attccgcgat ctggtccggg cgcagccggt
      121 aacgtctggc gacgctggcc actgtttcgt gcttcctggc tttcacccag gtgcggcgcg
      181 tgacgcgttc gggcgacagg ttcagctggc cgttatcggc gacatggctg gtcacgtcgt
      241 tttgcatctt ggccgaccgc ggaacgatca ggatggagcc cgccttgatc agcatgcgcg
      301 ggggaatggt gttcacggtg cgcaggtcgg cctcggtcat gcccacccgg cgcgccgcgt
      361 cggacgcgct catggtggcc ggcgcgctcc aggcggtcca actcgcgtac tggccgtcgg
      421 tgtaggcctc gaagttgcgc tggaagatct ttgcattgtc ccagggcagg agaatctcgg
      481 gcgtgccggc cgccaggatc accggtcgct tcatcgaagg gttcagcgcc ttgaagtcgg
      541 cgacggacac gtcggccaga cgcgcggcga gttccacgtc gatgtcgcgg ctgacggtga
      601 cagtctggaa gtaggggtgg ttctcgatca gaggaagctc ggcgcgcagg gcttccggat
      661 cggccacgat gttcttcacc gcctgcagct tgggaacgta gtagcgcgtc tcgttgggca
      721 tggtcaagtc gccgtagccg gtgcccaggc cgcttttctg gttgcgcgcg atcgcgcggc
      781 cgacactgcc ttcgccccag ttgtacgctg ccagcgccag gtgccagtcg ccgaacatgc
      841 cgtggagctt ttgcagatag tccagtgccg cgcgtgtcga cgcgagcacg tcgcgccggt
      901 cgtcgcggaa aacattctgc ttgagctcga agtagctgcc ggtggcgggc atgaactgcc
      961 acatgccggc tgcgcgcgcg ctggagacgg cctgcgggtt gaacgcgctt tcgatgaagg
     1021 gcagcagggc caactcggta ggcatgttgc ggcgctcgag ctcctccacg atatggaaca
     1081 ggtacttgcg ggagcgctcg gtcatgcgct ggatgtagtc gggccgcgac acgtaccact
     1141 gctcctgctg gcgcaccagg tcgctgtcca ggttgggcat ggcaaaaccg cggcggatgc
     1201 gcgcccacag gtcggccggg ggttccagct gcatgacggc gagggaggcg gcgtcggagg
     1261 cggtgatcgg ctttagcggg ccgcccggaa taacggacgg tgcggcgggt tcggcgggtt
     1321 tggccggcgt ggataccgcc gcaaccggat cggaaggcgg tggtgtggca cagccggcca
     1381 gccagagcag ggtggccagc gccgcgaaat gtagcagctt catctgaata agttcccttc
     1441 atttcggatg gtcataaaaa ccgcgacctc atcctccggg gcctgcgcgc gttcactcgc
     1501 actgccgaat gtaattgatc agcttcaggc cgtctggttc caggtccgga ctacttgaga
     1561 ttagtcaatg tgtattcatg tgtgctgccc gagccattgg cgttgcggcc ggccgggtca
     1621 acggttcgtg gatgagttcc cgggccggcg cgtaagcctt ggcgccggtg gcctcgcatg
     1681 gagggtcgac ccccaagtgg tcgatgaggt ggtgcatgac tagaatcgct tccagttgca
     1741 ggccgtcacg ctgcaagcag ggcaggacgg gctgcgtgtc gccggggtcc gctgcgaggg
     1801 ccccatgccc gttgtgcaac agccagaggt cattgtcttg taaagccggc agcgggacta
     1861 agttcatgag cgaccagatt ataggaatgc agcaatggtt tgaaacaccg cccggccgtt
     1921 acctgctggc ctgggagcgg gccgagttcg accgcgccgt tggcgacatc ttcggctttc
     1981 atgcgctgca actgggcttg cccgaactcg acacgcttca ggccaaccgc atgccgcaca
     2041 agtggctggc gctgagagag cccccgctgg agggttccgc gcagcccaag gcctcgctga
     2101 tcaccgactt cgcggccttg ccgttcgagg cgaacagcct ggacctggtc gtattgcccc
     2161 attcgctcga actcaacacc gacccgcacg cgaccctgcg cgaagtggag cgggtgctcg
     2221 tgccggaagg caaggtggtg atctgctgcc tgaacccggc gagcctgtgg gggctgcgcc
     2281 agcgccgcgc ccacctgtac cggcggctcg gattcggcga actctacctg cccgacgcgg
     2341 gggatttcat cggctattgg cggctgcggg actggttgcg gctgctcagc ttcgaggtgg
     2401 agtcgggcaa cttcgggtgc tatcgccccg ccatgagcac ggaaaaatgg ctggaccgct
     2461 acgattggct ggaccatgcg ggtgagcgct ggtggccgat cttcggcgcg gtgtatttcc
     2521 tggtcgcggt caagcgggtt cgcggcatca agctgatcgg ccccgcctgg aaggggtcaa
     2581 aagccatcgc cgccaagccc gtgccgctgg ccaaccgcag ccatcccgca catctggaag
     2641 aacacgtgtg aattcagtcg agatctatac cgacggcgcc tgcaagggca atcccggtcc
     2701 gggcggatgg ggcgtgctgc tgaaatccgg cggaactgaa aaagaactgt tcgggggcga
     2761 acgcctgacc accaacaacc gcatggagtt gatggccgtg atccaggcgc t
//
LOCUS       DASWIY010000495         2689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_352557, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000495 DASWIY010000000
VERSION     DASWIY010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352557"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VGA59_17795"
     CDS             <1..548
                     /locus_tag="VGA59_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721574.1"
                     /translation="ELGPIEYTFFSTVAFSTVLPAVASTAEWLDTMPRIHQAFGRWHP
                     IQIPHPRPAAIIVLIVTALSLAGLGAFREYLYPLVWISPGLVIVALQALAGRRTVLAP
                     LADGDCCGVATYSAAALICGFFWEMWNSGSLAHWEYSIPYVDACRLFEMPVLGYAGYL
                     SFGIECAVIAALALNGVKIEQ"
     gene            complement(910..2436)
                     /locus_tag="VGA59_17800"
     CDS             complement(910..2436)
                     /locus_tag="VGA59_17800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX9721575.1"
                     /translation="MLFARYGPRYRIYVTVIALLGTISAIVTSTTVNVALPNIMGAFG
                     IGQDRGQWIVTGNLAGQTVGQLLSAWLIDSFGQRKTFVVGLCVFVVSVLVAALSPNDL
                     TLVAARVVQGLIAGVLQSLTMYTLFSVFPPEKRGMAMGFFSINTILGPAVGPTVGGIL
                     IEHFDWRAVFYMGLPFSVVGILLGSLLMPEREETAKRAHFDWLGFALLCTAMSCLLIG
                     LSNGQREGWSSDFVLGSLAVAAGAGIAFLVWELHVPQPLVHLRVLAVGPFAAAACVAV
                     VFGIGQFGTTYLIPLFVQTIQGLTPLDAGLLLMPGALLLGIFMPLGGYLCDRLAARAL
                     LITGLSSFAVSTYWLHDVDVNTAFWWMLTCVVISRLGQALINPTLNASAMRSLHTTQL
                     RQGAGMINFFRQLGGAFGVNLLSVSLDRQTFFYSDRLTSMQTAGNSATTELLHSVERL
                     LAQAGASSDLQSAGALDFLGRVLYAQAHTLAFRDCFLIVTLIFMLALIPAWFMGRKAR
                     "
BASE COUNT          583 a          833 c          772 g          501 t
ORIGIN      
        1 ctgaattggg cccgattgaa tacacgttct tttccacagt cgctttctcg acggtcctgc
       61 cggcagtagc gagcaccgcg gagtggttgg acacgatgcc gcggattcac caggcctttg
      121 gacgctggca tccgatacag atcccgcacc cgagacccgc agccattatt gtgcttatcg
      181 tcacagcgct ttcacttgcc ggactcggcg ctttccgcga gtacctgtat ccgctcgttt
      241 ggatttcccc gggccttgtc atcgttgcgc ttcaggcgct cgccggccgg cgcactgtgc
      301 tcgcgccgct agccgacggc gactgttgcg gagttgcgac ctattcggcg gcagcactga
      361 tctgcgggtt cttctgggaa atgtggaact caggcagcct tgcccattgg gagtacagca
      421 tcccgtacgt ggatgcttgc cggctgttcg agatgccggt gctgggctac gcgggctatt
      481 tgtccttcgg catcgaatgc gcggtcatcg ccgcattggc gctgaacggg gtaaagattg
      541 aacagtgacg gggtgtcctt ggtgcgcaac ggattcgcgt ctatggtgac cttgccatca
      601 acaccgggac ctacaccttt attggcccgg ctcgtgacgc tgctggcaag ccaattagtc
      661 gtccgacggt cgcgaaccgg ccaattgctg cctctcactg atcggcacaa ccgagtcact
      721 caccgactga ttggaccgaa tggggtgctc gccgatcgat acacaatgtg gcaagccgct
      781 cctgctgcgc gattggtgta atccgcgcga aacatccttc aattgcttag cgagacttgg
      841 aaatgggctg gaataggtgg ttagcgcaat tcacgcacga tgtgtcaatc ttgttcgcgc
      901 accaacaccc tatcgcgcct tgcgccccat aaaccatgcc ggaatcaatg ccagcatgaa
      961 tatcagcgta acgatcagga aacagtcgcg aaatgccaat gtgtgagcct gcgcatacag
     1021 aacgcgcccg aggaaatcta gcgcgcccgc ggattgcagg tcagacgatg ccccggcctg
     1081 cgccagcagc cgttccactg aatgcagcag ttccgtagtc gcgctgttac ctgcagtttg
     1141 catgctcgtc agtctgtcgc tgtaaaaaaa ggtttggcga tcaagcgaga ccgacaacag
     1201 gttgacgcca aacgcaccgc cgagttgccg aaagaaattg atcatgcccg cgccctgccg
     1261 gagttgtgtt gtatgcagcg agcgcatcgc gctggcgttc agcgtcgggt tgatgagcgc
     1321 ctgcccaagg cgacttatca cgacgcaggt gagcatccac cagaatgccg tgttcacatc
     1381 gacgtcatgc agccaatacg tcgagacggc gaaggacgac aaacctgtaa tcaggagcgc
     1441 gcgcgccgcc agccggtcac acaaatatcc acccagcggc atgaatatcc ctagaagcag
     1501 agcgcccggc atgagcagca gcccggcatc gagaggcgtc aatccctgaa ttgtctgtac
     1561 gaataagggg atgaggtatg tcgtgccgaa ttgcccgatt ccgaagacaa cggcgacgca
     1621 ggctgccgca gcaaaggggc cgaccgccag cacgcgaaga tggaccagcg gctgcggcac
     1681 gtgcagctcc cagacaagaa acgcgattcc cgcgcccgca gccactgcga gcgatcccag
     1741 tacgaaatcg gagctccagc cttcgcgctg accgttggac aacccaatca gcaggcaaga
     1801 catcgccgtg cacagcaacg caaagcctag ccagtcgaag tgcgcgcgct tggctgtttc
     1861 ctcgcgctcc ggcatcaaca ggctgcccag gaggatgccg acgacgctga acggcagccc
     1921 catgtaaaag acggcgcgcc agtcgaagtg ctcgatcagg atgcccccca cagtcggccc
     1981 gaccgcaggt ccgaggattg tgttgatact gaagaatccc atcgccatcc cacgcttttc
     2041 cggcgggaac acgctgaaca aggtatacat cgtcagcgat tgcagcacgc cggctatcaa
     2101 gccttgaacc acccgtgccg cgaccaacgt cagatcgttc ggactcagcg cggccaccag
     2161 caccgaaacg acaaagacgc acagcccgac aacgaaggtc ttgcgctgac caaagctgtc
     2221 gatcagccag gcgcttagca actgtccgac cgtctggccc gccaaatttc ccgtgactat
     2281 ccactggccg cgatcctgcc cgatgccgaa tgcccccatg atgttgggca acgcgacgtt
     2341 gactgtcgtg ctggtcacga ttgcagatat cgtccccagc aacgcaatca cagtgacgta
     2401 aatgcgatag cgtggcccgt agcgggcgaa cagcacctca gtagtctcag cggttgcgga
     2461 catcgatgca gactttcacc agcatgcgtg accgcagtgc ccgagcggcc atcgagaaaa
     2521 ttgtactaag tggctatcgg gctcaagtcg gccattaccg gtcggtcgca ccctttcctc
     2581 cgcaggggaa aaaaggcgca gaatatgctg ggagggttac cagcaggatc gccccggagt
     2641 tagcatcgac cccgtgccgg cgcgatcacc gcggagcgca tcctcaagg
//
LOCUS       DASWIY010000496         4682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_1477166, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000496 DASWIY010000000
VERSION     DASWIY010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477166"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..446
                     /locus_tag="VGA59_17805"
     CDS             108..446
                     /locus_tag="VGA59_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX9721576.1"
                     /translation="MSDANSYYAHHIFFCLNQRGNGEACCAEHHAQEAYDHCKAKVKS
                     MGLNAPGKVRVNKAGCMDRCAAGPVAVVYPEAVWYTYVDQHDIDEIVESHLKNGQVVQ
                     RLLTPPHLGR"
     gene            443..1072
                     /locus_tag="VGA59_17810"
     CDS             443..1072
                     /locus_tag="VGA59_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9721577.1"
                     /translation="MNSLTRRYQLAGAAGMIEVLRDEPAAGKPRHGMAVIAHPHPLFG
                     GTMDNKVVQTMARAFVQAGWTALRFNFRGVGASQGEHDEGRGELDDMLALIAAEAPEG
                     PLALAGFSFGSFVASRAAQALWPARSIEKIVLVGTAASRFEVATLPPEAQERTLVVHG
                     EQDDTVPLASVMDWARPQSLPVTVVPGGGHFFHGQLPLLKSLVARHLRS"
     gene            1098..2243
                     /locus_tag="VGA59_17815"
     CDS             1098..2243
                     /locus_tag="VGA59_17815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX9721578.1"
                     /translation="MLALAAPLLALLCFTAAAQVPQPPEIAARSYLLLDVTADQLLAG
                     RDIDAPIEPASLTKLMTAYLVFDALRSKKLDLKQALPVSERAWKTPGSRMFIDPKMQV
                     PVEDLLKGMIVQSGNDATVALAEGVGGTYERFVQLMNDQAKAMGLKATSYRNPEGLTE
                     AGHSTTARDLSVLSTRLIQDFPDYFAFYSIKKYRYQGTPVANDTNRNMLLFRDPTVDG
                     LKTGHTNAAGYCLIATAKRDFPNVGSRRLLAIVLGSTSENARANEAQKLLNWGYTAFD
                     AVKLFDANQPVVTPEVWKGTEATVKLGRTQPLVVAIPSGTAGRIKTQVARPDPLVAPL
                     AKGQQVGTLKISSGDQPLLDVPLFVLEAVEEAGVFRRAWHALRLWIK"
     gene            2512..2889
                     /gene="rpsL"
                     /locus_tag="VGA59_17820"
     CDS             2512..2889
                     /gene="rpsL"
                     /locus_tag="VGA59_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX9721579.1"
                     /translation="MPTINQLVRQGREVEKTKSKSPAMQNSPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVISYIGGEGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGSL
                     DLQGVKDRKQSRSKYGAKRPKKA"
     gene            3075..3548
                     /gene="rpsG"
                     /locus_tag="VGA59_17825"
     CDS             3075..3548
                     /gene="rpsG"
                     /locus_tag="VGA59_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX9721580.1"
                     /translation="MPRRREVPKREILPDPKYGNVELAKFMNVIMQGGKKAVAERIIY
                     GALEQIEKKNPGKDPVEAFTMAINNVKPMVEVKSRRVGGANYQVPVEVRPVRRLALAM
                     RWLKEAAKKRGEKSMAMRLANELMEATEGRGGAMKKRDEVHRMAEANKAFSHFRF"
     gene            3619..>4682
                     /locus_tag="VGA59_17830"
     CDS             3619..>4682
                     /locus_tag="VGA59_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9721581.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWKGMDLSFPEHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQANKYKVPRMAFVNKMDRVGANFFKVYDQMKA
                     RLKANPVPIVIPIGAEEHFTGVVDLRKMKAIYWDEASQGMKFTFAEIPAELLELSKEW
                     REKMVEAAAEANEELMNKYLEEGELTEAEITQGLRIRTIAGEIQPMLCGTAFKNKGVQ
                     RMLDAVIELMPSPVDIPPVTGMDDDEKPVTRRADDSEKFSALAFKLMTDPFVGQLTFV
                     RVYSGVLSKGDSVYNPIRGK"
BASE COUNT          945 a         1474 c         1498 g          765 t
ORIGIN      
        1 cgctttcatg ggctggccca gtggctgggc tggctgtggc cttatgccgc cctggcttat
       61 gttctggtgc gcgtgtcgcg aagcgacgcg caaaatagaa tgcccgcatg agcgatgcga
      121 attcctacta cgcgcaccac atcttttttt gcctgaacca gcgcggcaac ggtgaggcct
      181 gctgcgcgga gcatcacgcc caggaggcct acgaccactg caaggcaaag gtcaagtcca
      241 tgggactgaa cgccccgggc aaggtgcgtg tgaacaaagc gggttgcatg gaccgatgcg
      301 ccgccggccc ggtggccgtg gtctatccgg aggcggtctg gtacacctac gtggaccagc
      361 acgacatcga cgagatcgtc gagtcgcacc tgaagaacgg ccaggtcgtg cagcggctgc
      421 tgacgccgcc gcacctggga cgatgaattc cctgacgcgg cgctaccagc tcgccggtgc
      481 ggccggaatg atcgaagtcc tgcgcgatga acctgccgcg ggcaagcccc ggcatggcat
      541 ggccgtcatc gcccatccgc atccgctgtt cggcggcacc atggacaaca aggtggtgca
      601 gaccatggcc cgcgctttcg ttcaggcagg ctggaccgcg ctgcgcttca atttccgcgg
      661 cgtcggcgcc agccagggag agcacgacga ggggcggggc gaactggacg acatgctggc
      721 cctgatcgca gccgaggcgc ccgaagggcc gctggcgctg gctggcttct cgttcggctc
      781 cttcgtcgcc agccgggccg cgcaggcgct ctggccggcg cgcagcatcg aaaagatcgt
      841 cctggttggc actgccgcct cccgcttcga ggtggccacg ctgccgcccg aagcgcagga
      901 acgcacgctg gtggttcacg gcgagcagga cgacacagtg ccgctggctt ccgtgatgga
      961 ctgggcgcgg ccccagtcac ttccggttac ggtggttccc ggaggcggtc atttctttca
     1021 cggacaatta ccgctcctga aaagcctggt ggcgcgccac cttcgttcct gacctgttct
     1081 tgcccatgaa aaaaataatg ctggcgctgg ccgcgcccct cctggccttg ctgtgtttta
     1141 ccgccgccgc ccaggtgcct cagccccccg aaatagcggc gcgcagctac ctcctgctgg
     1201 atgtgacggc cgatcagttg ctggccggcc gggacatcga cgcgccgatc gaaccggctt
     1261 cgctgaccaa gctgatgacc gcatacctgg tgttcgacgc gctgcgaagc aagaagctgg
     1321 acctcaagca ggcactgccc gtcagcgaac gcgcctggaa gacgccgggg tctcgaatgt
     1381 tcatcgatcc caagatgcag gtgccggtgg aagacctgct caagggcatg atcgtgcagt
     1441 cgggcaacga cgccacggtg gcgcttgccg aaggggtggg cggcacttac gagcgcttcg
     1501 tgcagctcat gaacgaccag gccaaggcga tggggctgaa ggctaccagc taccgcaacc
     1561 ctgaggggtt gaccgaggcc ggccattcga ccaccgcgcg cgacctgagc gtgctgtcca
     1621 cccggctgat acaagacttc cccgattact ttgcctttta ctcgatcaag aaataccgct
     1681 accagggcac tcctgtggcc aacgacacca accgcaacat gctgctgttt cgggatccca
     1741 cggtcgacgg cctcaagacc ggccatacga acgcagcggg gtattgcctg atcgccaccg
     1801 ccaagcgcga ctttcccaac gtgggctcgc gccggctgct ggcgatcgtg ctgggttcga
     1861 ccagcgagaa tgcccgcgcc aacgaggcgc aaaaactgct caattggggc tatacggcgt
     1921 tcgacgccgt caagctgttc gacgccaacc agccggtagt gacgcccgag gtctggaagg
     1981 gcacggaagc aaccgtcaag ctgggccgca cgcagccgct ggtcgtggcg attcccagcg
     2041 gcacggccgg acggatcaag acgcaggtgg cgcgccccga cccgctggtg gcgccgctgg
     2101 ccaagggcca gcaagtggga acgctcaaga tcagctcggg tgaccagccg ctgctggatg
     2161 tgcccctgtt cgtgctggaa gccgtggaag aggccggcgt gttccgccgg gcgtggcacg
     2221 ccctgcggct gtggatcaag tgacatcccg tggctgcggc gcagcctagg gatgtcatcc
     2281 cggcgaaagc cgggatccag ggcgtttttg tggctgcggc gcagcctagg gatgtcatcc
     2341 cggcgcaggc cggaatcccg cgcatatcgc gtcatcccgg gcttgacccg ggatccatgt
     2401 gccgttgagc atttacatca atcgggctat acttgaaggc tttcccgcat ttggggcggg
     2461 aaaagcgcgt aatttttcag cttgatccat atcgacgttt agggacgttc aatgccaacc
     2521 atcaaccaac tcgtgcgtca agggcgggag gtcgaaaaga ccaaatccaa gagcccggcg
     2581 atgcagaaca gcccccagcg ccggggcgtg tgcacgcgtg tgtacacaac gacgccgaag
     2641 aagccgaact ccgcccttcg caaggtcgcc aaggtccgcc tgaccaacgg cttcgaagtc
     2701 atttcctaca tcggcggcga aggccacaac ctgcaggagc actcggtggt gctcgtgcgc
     2761 ggcggccgcg tcaaggacct gcccggtgtg cgttaccaca tcgtgcgcgg atcgctggac
     2821 ctgcaaggtg tgaaagaccg caagcagtcg cgctccaagt acggtgccaa gcgcccgaag
     2881 aaggcctgat gggctttggc ccgaagtgag tcaatcgttt tgtcggtgct cgaaccaccc
     2941 tggcaggtga gccgagcgaa gtgacccgaa gcacggaatc gtccgtgtgg gtcgagtaag
     3001 cgagggcccg gctgggctct ctgggccccc gaaaaaggga caagccagct gaagcaaatt
     3061 gaagaggtga aaaaatgcca cgtcgtcgcg aagtccctaa acgtgaaatc ctgccggatc
     3121 ccaagtacgg caatgtcgag ctcgccaagt tcatgaacgt catcatgcaa ggcggcaaga
     3181 aggcggtcgc cgagcgcatc atttacggcg cgctcgagca gatcgagaaa aagaacccgg
     3241 gcaaggaccc ggtggaagcc ttcaccatgg cgatcaacaa cgtcaagccc atggtcgagg
     3301 tgaagtcccg ccgcgtcggc ggcgccaact accaggtgcc ggtcgaagtc cggccggtgc
     3361 gccgtctggc gctggcgatg cgctggctca aggaagccgc caagaagcgc ggcgaaaagt
     3421 ccatggcgat gcgcctggcc aatgaactca tggaagccac ggaaggccgg ggcggcgcca
     3481 tgaaaaagcg tgacgaagtt caccgcatgg cagaagcgaa caaggctttc agccacttcc
     3541 gcttctagag accgtcccca tctacgccaa gcgctggctc gccacaagcg ggccagcgac
     3601 cattaacgaa agatcatcat ggctcgcaag actcccatcg agcgctaccg caacatcggc
     3661 atatcggcgc acatcgacgc cggcaagacg acgaccaccg agcgcatcct gttctacacc
     3721 ggcgtgaacc acaagatcgg cgaagtgcac gacggcgccg ccacgatgga ctggatggag
     3781 caggagcagg agcgcggcat cacgattact tccgccgcaa ccacctgttt ctggaagggc
     3841 atggacctgt ccttccccga gcaccgcatc aacatcatcg acaccccggg ccacgtggac
     3901 ttcaccatcg aggttgagcg ttccatgcgt gtgctggacg gcgcgtgcat ggtgtactgc
     3961 gccgtgggcg gcgtgcagcc ccagtcggag accgtctggc gccaggccaa caagtacaag
     4021 gttccccgga tggcgttcgt caacaagatg gatcgcgtcg gtgcgaattt cttcaaggtc
     4081 tacgaccaga tgaaggcgcg cctgaaggcc aaccccgtgc cgatcgtcat cccgatcggt
     4141 gccgaagagc acttcacggg cgtggtcgac ctgcgcaaga tgaaagccat ctattgggac
     4201 gaagcgtccc agggcatgaa gttcaccttt gcagagattc ccgccgagct gctggagctg
     4261 tccaaggagt ggcgcgagaa gatggtcgag gccgcggccg aagccaacga agagctgatg
     4321 aacaagtacc tggaagaagg cgaactgacc gaggccgaga tcacccaggg cctgcgcatc
     4381 cgcacgatcg ccggcgagat ccagccgatg ctgtgcggca ccgcgttcaa gaacaagggt
     4441 gtgcagcgca tgctggacgc cgtgatcgaa ctcatgcctt cgccggtgga cattccccct
     4501 gtcacgggca tggacgacga cgagaagccg gtcacccgcc gcgccgacga cagcgagaag
     4561 ttctcggcgc tggcgttcaa gctgatgacc gacccgttcg tgggccagct caccttcgtg
     4621 cgcgtgtatt cgggcgtgct gtcaaagggc gacagcgtct acaacccgat ccgcggcaag
     4681 aa
//
LOCUS       DASWIY010000497         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_730282, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000497 DASWIY010000000
VERSION     DASWIY010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730282"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGA59_17835"
     CDS             <1..193
                     /locus_tag="VGA59_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9721582.1"
                     /translation="DLVNATVGFEELAQLTDKPPKSLHRMLSPNGNPSMDNLAAIFGA
                     VSSRLNVGLEAHTVETAGG"
     gene            186..>2222
                     /locus_tag="VGA59_17840"
     CDS             186..>2222
                     /locus_tag="VGA59_17840"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX9721583.1"
                     /translation="MVELNAQQLEAVQHGTGELAEDTRPLLVIAGAGSGKTSTLAHRV
                     ANLIRHGADPQRMMLLTFSRRAAQEMERRVGGVLQKALGLPQQQSLPNLPWSGTFHSI
                     GARLLREYAGRIGLEESFTIQDRGDAEDLMGMVRHDIGLAETKNRFPLKGTCLGIYSR
                     VLNTRDPLERVLQDTYPWCGQWERQLKKLFGAYVEAKQQQNVLDYDDLLLFWAEMMAE
                     PALAQEVGSRFDHLLVDEYQDTNRLQSEIILGMKPTGRGVTVVGDDAQSIYSFRGATV
                     RNILDFPHQFTQAARNVAPTLAADAAALPPEGARSPRDGPSVRVVTLERNYRSTQPIL
                     DASNAVIGEARERHAKNLWTDRSCGPKAQLLLVPDETGQARWVADQVLRQREAGTALK
                     SQAVLFRTSAHSAALELELARRNIPFVKFGGLKFLEAAHVKDMLAVLRFAQNPRGRLA
                     GFRVTQLIPGFGPATAARLLDAMGEAAEPAQTLRQFDAPAQARDEWAKFAALYTQLRN
                     PDMAWPADLELAKAWYLPQLERIHDDAPARKLDVEQLVRLAAGYSNRERFLTELTLDP
                     PQATSDQSGVPHLDEDYLILSTIHSAKGQEWKAVHVLNVVDGCIPSDMATGTPEELEE
                     ERRLLYVAMTRARDHLHLIVPHRFYVTQQSAAGDRHIYAGRTRFISEAMAEKFER"
BASE COUNT          413 a          752 c          731 g          326 t
ORIGIN      
        1 cgacctggtc aacgcgacgg tcggctttga ggaactggcc cagcttaccg acaaaccccc
       61 aaaaagcctg cacagaatgt tgtcgcccaa tggcaacccc agcatggata acctggctgc
      121 gattttcggt gcggtcagtt ctcgcctgaa cgtcggcttg gaagctcaca cggtcgagac
      181 ggccggtggt tgaactcaat gcccagcaac tggaggccgt gcaacacggc accggcgagc
      241 tagccgagga cactcgccct ttactcgtca tcgccggagc cggctcgggc aagaccagca
      301 cgctggcgca tcgcgtcgcc aacctgatcc gccacggcgc cgacccgcag cgcatgatgc
      361 tgctcacctt cagccggcgc gccgcgcagg agatggagcg gcgcgtcggc ggcgtgctgc
      421 aaaaggcgct gggcctgccg cagcagcaat ccctgcccaa tctgccatgg tccggcacct
      481 tccacagcat cggggcgcgc ctgctgcgcg aatatgcggg ccgcatcggc ctggaggaat
      541 ccttcaccat tcaagatcgc ggcgatgcgg aagacctcat gggcatggtg cggcacgaca
      601 tcggcctcgc ggaaacgaag aaccgtttcc cgctcaaagg gacgtgcctg ggaatctatt
      661 cgcgcgtgct caacacgcgc gatccgctgg agcgggtgct ccaggacact tatccctggt
      721 gcggccagtg ggagcgccag ctcaagaaac tcttcggcgc ctatgtcgaa gccaagcagc
      781 agcagaacgt gctggactac gacgacctgc tgctgttctg ggccgagatg atggccgaac
      841 ccgcgttggc gcaagaggtg ggcagccggt tcgaccactt gctcgtggac gaataccagg
      901 acaccaatcg gctgcagtcc gaaatcatcc tgggaatgaa gcccacgggc cggggcgtga
      961 cggtggtggg cgacgacgcg caaagcatct actcgttccg cggcgccacg gtgcgcaaca
     1021 tcctggactt tccccatcaa ttcacgcaag ccgcccggaa tgtggccccc acgctggcgg
     1081 ccgatgccgc tgcgctgccc cccgaggggg ctcgctcacc tagggacggc ccgtcggtgc
     1141 gcgtggtgac gctggagcgc aactatcgca gcacgcagcc catcctcgat gcgagcaacg
     1201 ccgtgatcgg agaagcgcgc gagcgccacg ccaagaacct gtggacggac aggtcctgcg
     1261 gccccaaggc gcaattgctg ctggttcccg acgagaccgg ccaggcgcgc tgggtggccg
     1321 accaggtgct gcgccagcgc gaagccggca ccgcgctcaa gagccaggcc gtgctgttca
     1381 ggaccagcgc ccacagcgcg gcgctggagc tggaactggc gcgccgcaac atcccgttcg
     1441 tgaaattcgg gggcctgaaa ttcctggaag ccgcgcatgt gaaggacatg ctggccgtgc
     1501 tgcgcttcgc ccagaacccg cgcggccgac tggcgggttt tcgcgtgacg cagctcatcc
     1561 cgggtttcgg gccggccacg gcggcgcgac tgctcgatgc catgggggaa gcggccgaac
     1621 cggcgcaaac gctgcggcag ttcgacgcgc ccgcgcaggc ccgggacgag tgggcgaaat
     1681 tcgcggcgct ctacacgcag ttgcgcaatc ccgatatggc ctggcccgcc gacctggaac
     1741 tggccaaggc ctggtacctg ccgcagctcg agcgcatcca cgacgacgcg ccggcgcgca
     1801 agctcgacgt ggaacagctg gtgcgcctcg ccgccggcta ttcgaaccgc gagcggttcc
     1861 tcaccgagct gacgctggac ccgccgcaag ccaccagcga ccagtccggc gtgccgcacc
     1921 tggacgagga ctacctgatc ctgtcgacca tccacagcgc gaagggccag gagtggaagg
     1981 cggtgcatgt gctcaacgtg gtcgatggct gcatccccag cgacatggcc accggcacgc
     2041 cagaggagct cgaggaagag cggcgcctgc tctatgtggc gatgacgcgc gccagggatc
     2101 acctgcacct gatcgtgccg caccgtttct acgtgacgca gcagtcggcg gccggcgaca
     2161 ggcacatcta tgccggccgc acccgcttca tcagcgaagc catggcggag aagttcgagc
     2221 gg
//
LOCUS       DASWIY010000498        20335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_2585852, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000498 DASWIY010000000
VERSION     DASWIY010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 20335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20335
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2585852"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1006)
                     /locus_tag="VGA59_17845"
     CDS             complement(230..1006)
                     /locus_tag="VGA59_17845"
                     /inference="COORDINATES: protein motif:HMM:NF026241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M91 family zinc metallopeptidase"
                     /protein_id="HEX9721584.1"
                     /translation="MNFLTQYNIAVNPVGLAGAAAQTYTRKVTEHLTWIQRTTSGRIL
                     LASIKFHGKPVVIQPYTGGKCDAVGGGQMVGGAIQGAVWYSPDTFSVHGACSATKSAK
                     NRGLYWDEVLFHELVHVFRNVSGKWSKRPVFGGLNKYDDSEEFVAVLLTNIYISDRSN
                     KIKSGLRRNHQSFDALEPKLAQPWGFFTSGTQTFGLIQSFVADNPGISKRIANDVATA
                     PFNPIADYFADRHRAERLSREALPRDVASIMSDIASAIGL"
     gene            1453..2238
                     /locus_tag="VGA59_17850"
     CDS             1453..2238
                     /locus_tag="VGA59_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721585.1"
                     /translation="MALSISSPINVLVLDISQSVGVKGKNRYGDVIRVQEALNTIPAS
                     EGTSSTLLSLDGIAGPKTCDAIQKLQLKHFGWKGADGRIDPGGSTAQLMSGLLVRYGG
                     ILWTIRRLESAVALKSNLQDVRTIESKDRIFEIADVTGRRRALYHFRPAEVTYIPRAA
                     DVPALRERPEVNMFNTVLPCGPHAFATADAMYSEFSPDADHAVIQLTLRPRAAHIAPG
                     VLSLHVNHQWIKPMSTPGARARMAGIFGFLRDDSAVGGKTPRW"
     gene            complement(2279..2611)
                     /locus_tag="VGA59_17855"
     CDS             complement(2279..2611)
                     /locus_tag="VGA59_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX9721586.1"
                     /translation="MPFYLTRFSYTPATWAKLIKNPEDRRTAASQYIESVGGKLHGFW
                     YAFGEYDGYTLWEAPDNVSMAAAALAIGAGGALSSLQTTALLTVEETVAALKKASTVK
                     YWSPGDKK"
     gene            complement(2736..3050)
                     /locus_tag="VGA59_17860"
     CDS             complement(2736..3050)
                     /locus_tag="VGA59_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721587.1"
                     /translation="MRSEVTVTFDGAAAPVRMDLNREEPMPNDAARAWLDRQFIELDC
                     DPLRATGKVLTADKVVVVAQSAGPAKFADGKWAQEFARAASGALGKPVVHVDVPSLSI
                     SY"
     gene            complement(3181..3399)
                     /locus_tag="VGA59_17865"
     CDS             complement(3181..3399)
                     /locus_tag="VGA59_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721588.1"
                     /translation="MAVHADPYESLQAQYRAATAQERSAWKALSDTELGAVERGKAYA
                     RWAAAAERIKVLAIRMRDLGAPVQSTPS"
     gene            complement(3551..3814)
                     /locus_tag="VGA59_17870"
     CDS             complement(3551..3814)
                     /locus_tag="VGA59_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721589.1"
                     /translation="MNKVEGKLSAWHRLYLQLQDARQRLKQLQATPADSTRACLEAEV
                     SRLQQESDSELEAFNSEFIEAKGSTGAGRSPNSRQGLTIRPRH"
     gene            complement(3975..4892)
                     /locus_tag="VGA59_17875"
     CDS             complement(3975..4892)
                     /locus_tag="VGA59_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HEX9721590.1"
                     /translation="MEIAIVILVIAVIFITRSIKVVPQQNAWVVERLGKYHAALAPGL
                     NFLVPFIDRVAYKHSLKEIPLDVPSQVCITRDNTQLQVDGILYFQVTDPMRASYGSSN
                     YIMAVTQLAQTSLRSVIGKLELDKTFEERDIINAQVVQAIDEAALNWGVKVLRYEIKD
                     LTPPKEILHAMQSQITAEREKRALIAASEGRRQEQINIATGEREAFIARSEGEKQSII
                     NNAQGEAASITAVADATAMAIERIAAAIRQPGGEQAVQLKVAEKAVEAYSKVATDSTT
                     TLIVPSNMTEVSALIASAMRMVQTGKAAA"
     gene            complement(4923..5348)
                     /locus_tag="VGA59_17880"
     CDS             complement(4923..5348)
                     /locus_tag="VGA59_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX9721591.1"
                     /translation="MAESTIWWLMAGTAIAVELVTGTFYLLMLAIGLAAGALAAHIGA
                     GTATQLLAAAVVGGSSVVGWYYAKKRRPEDPSVRADRSVNLDVGSTVHIERWNPDGTA
                     TVKYRGATWTAVHREGEIPVAGPHRVAELIGSRLLVDPI"
     gene            5582..7858
                     /locus_tag="VGA59_17885"
     CDS             5582..7858
                     /locus_tag="VGA59_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HEX9721592.1"
                     /translation="MKFRFPIVIIDEDFRSENTSGLGIRALAQAIETEGFEVLGVTRY
                     GDLSQFAQQQSRASAFILSIDDEEFTPGPDLDPAVVNLRSFIEEVRRKNADVPIYVYG
                     ETKTSRHIPNDILRELHGFIHMFEDTPEFVARHIIREAKSYLEGVQPPFFKALLDYAE
                     DGSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVDELGQLLDHTGPVAA
                     SERNAARIFNADHCFFVTNGTSTSNKIVWHHTVAPGDVVVVDRNCHKSVLHAIIMTGA
                     IPVFMKPTRNHFGIIGPIPQSEFEPAAIRAKIRANPLLKGVDPDSVKPRVMTLTQSTY
                     DGVIYNTETIKKMLDGYVDTLHFDEAWLPHAAFHPFYGPYHAMGKNRVRPRESLTFAT
                     QSTHKLLAGISQASHVLVQDSQNRKLDRHLFNEAYLMHTSTSPQYAIIASCDVAAAMM
                     EPPGGTALVEESILEALDFRRAMRKVDEEYGKDWWFKVWGPDKLVEEGIGRADDWIIK
                     GEARTAKWHGFGNLAEGFNMLDPIKSTIVTPGMDLNGKFAKTGIPASIVTRFLAEHGI
                     IVEKTGLYSFFIMFTIGITKGRWNTMLTALQQFKDDYAKNQPMWRILPEFCQQFPRYE
                     RMGLADLCQHIHELYAKYDIARLTTDMYLSDLTPAMKPSDAYAFIAHRKTERVAIDEL
                     EGRITTSLVTPYPPGIPLLIPGEVFNRKIVDYLKFSREFNAQCPGFETDIHGLVEEAG
                     PDGALRYYADCVQNGDRA"
     gene            complement(8060..8857)
                     /gene="fabI"
                     /locus_tag="VGA59_17890"
     CDS             complement(8060..8857)
                     /gene="fabI"
                     /locus_tag="VGA59_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX9721593.1"
                     /translation="MGFLAGKKLLITGVLSNRSIAYGVAKACRAQGAELAFSYVGERF
                     KERIAEFAADFDSQLIFDCDVGDDAQIDRLFKDLSNAWPTFDGFVHSIGYAPREAISG
                     SFLEGLTRENFRIALDISAYSFPAMAKAALPYLNDKSAILTLTYLGAMRAIPNYNTMG
                     LAKASLEASVRYLAEAVGQLPDGRSMRANGISAGPIRTLAASGIKDFGKLLSICASAS
                     PLRRNVTIEDVGNVAAFLLSDLASGVTAEITYVDAGFSQTAVAVVET"
     gene            8897..10690
                     /locus_tag="VGA59_17895"
     CDS             8897..10690
                     /locus_tag="VGA59_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9721594.1"
                     /translation="MRGLLFLLACLVAGPSSAGYAYAQFGDIKYPPGFTHFDYVNPNA
                     PKGGEIRMVPPTRPSNFDKFNPFTLKGNAPYDIDLLFDSLLVGNSDEPTTAYGLLAED
                     VEVAPDRLSATFRLNPKARFHDGKPVLAADVVHSFKTLISEEAAPQFRTIYAEVKDAT
                     AVAERTVRFDFSSPNPELPLVVGGMSVFSREWGKGKPFDEVVSEIPIGSGPYRIANPR
                     MGRDITYVRDPNYWAADLPTRKGQFNFDRITFKIYLDETSRFEGLKAGEFDFMREFIS
                     RNWARQYKGKHFNSGELAKRAFENRNPGDFQGYIFNLRKPKFQDIRVRKAIGLAMDFE
                     WMNKQLFYGIYKRVNGYFPNSDFHAEGLPKPDELALLEPLRDKLRPEVFGPAPVSHST
                     DPPGSLRENLRQAQALFKEAGWEYRDGALRNANGEAFTMEFLNDQPSLIRIVGPFQKA
                     LEKLGVQLIYRVVDFSLSKQKMDAFDFEVTTLRLPGRTAPGGELLELFGSKAAATPGS
                     SNVWGIADPAVDALLQKVVTARTRPDLGAAMRALDRVLSHGYYSVPQYYGDAFLVGYR
                     PRPFVLPPRIPPYYQVDSWALSTWWASPENR"
     gene            10698..11732
                     /gene="yejB"
                     /locus_tag="VGA59_17900"
     CDS             10698..11732
                     /gene="yejB"
                     /locus_tag="VGA59_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HEX9721595.1"
                     /translation="MTSYILKRLLLMIPTLFGILLVTFAIVQFVPGGPVEQMVAQLQG
                     RDTGGERAAAAGAGYRGRQGVDEKRIEEIRKLYGFDKPAHERFLQMLGQFARFDLGQS
                     FYQHKDVWELVKEKMPVSISLGLWTFFLSYLIAVPLGVAKAVRAGSRFDFVTTLVVLV
                     GYAIPGFVLGVALLVIFGGQLQWFPLRGLTSSNWEQLGWGARIVDYLWHIALPVTAMV
                     FGSFAVTAMLTKNSFLEEIRKQYVLTARAKGLDERKVLWKHVFRNALIPIITGFPAAF
                     IGAFFTGSLLIETLFSLDGLGLLSYESVIRRDYPVVLGTLYFFTLIGLVTKLISDLSY
                     VLVDPRVKFD"
     gene            11827..12894
                     /locus_tag="VGA59_17905"
     CDS             11827..12894
                     /locus_tag="VGA59_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9721596.1"
                     /translation="MPAAAAEPASAPPAAFVSPGRRAWQRFRRNKLGFYSLVIFSVLV
                     VLSLFAEVLSNDTPLVVRYEGQWYFPIAKDYPETTFGGDFHTPTDYLDPFVRDKLTQG
                     GNWALYPPNHYGPKTLNYFASQPNPTAPSGENIFGTDDRGRDLLAQLIYGFRVSVLFG
                     LALTVVGVVIGVITGAVQGYFGGKTDLAFQRFIEIWGSMPELYLLIIFSAVFARSVGL
                     LLILLSLFGWMGLSDYVRAEFLRNRQMDYVRAAKALGVGNGRIMWKHILPNSMTPVVT
                     FLPFRMSAAILALTSLDFLGLGVPPGTPSLGELLNQGKANLDAWWISLSTFGVLVLTL
                     LLLTFMGDALRDALDPRKAEK"
     gene            12891..14579
                     /locus_tag="VGA59_17910"
     CDS             12891..14579
                     /locus_tag="VGA59_17910"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX9721597.1"
                     /translation="MTTPTLAAGAAALPPEGAFASRGGPSPLKLLLDVKGLRVAFGEK
                     EVVRGVDFSIAPGEKLALVGESGSGKTVTALSLLGLVQNARVSGSAMFAGLADPANPR
                     DLLSMSERELLATRGREIAMIFQEPMTALNPLYTVGDQVAEVLQLKQGLSGREAWEAA
                     VGALAATGIPEPERRAFAYPHQLSGGQRQRAMIAMALSGRPKLLLADEPTTALDVTLR
                     AQILDLLAEVQHQNGMAVLLITHDLNLVRKFADRVAVMENGYLVEHGTVDEVFANPRH
                     AYTRKLIESRPVRDVTEAPAPPEPPVMSASGVEVSYPVPIAGFRGWFRKGRFVAVRGA
                     GFSIAPGRTLGVVGESGSGKSTLALAALGLMPHSGELEVVGRRWGPDSAGNKAIRRLV
                     QVVFQDPYSSLSPRMTVEEIVGEGLLVHEPGLPEAGRRERVIQALGEVGLSEEQFPGL
                     LQRYPHEFSGGQRQRLAIARALIVQPRLLILDEPTSALDVTIQKQVLKLLQRLQREKR
                     LSYMLITHDVDVIRAMAHDVVVMKDGDILESGTVTQVLDAPRQDYTRILVAAAS"
     gene            14581..14991
                     /gene="arfB"
                     /locus_tag="VGA59_17915"
     CDS             14581..14991
                     /gene="arfB"
                     /locus_tag="VGA59_17915"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX9721598.1"
                     /translation="MKARPPTAIPESEVEITAVRAQGAGGQNVNKVSSAIHLRFDIPN
                     SSLAPDHKERLLALADQRITRDGVLVIKAQSHRSQDMNRAEALGRLQELVDSVAAPPR
                     PRRPTKPTLGSKKRRLEAKNLRSQVKLLRSRVSE"
     gene            15233..15847
                     /gene="rimP"
                     /locus_tag="VGA59_17920"
     CDS             15233..15847
                     /gene="rimP"
                     /locus_tag="VGA59_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX9721599.1"
                     /translation="MALQEIVEQTVTGLGYELVEIERSAGGLLRITIDLPWSPGVERF
                     VNVEDCEKVTRQLQFALEVEGAEYKRLEVSSPGIDRPLRTEKDFERFAGELVDITLKA
                     PMGAAAGGQVAANRKKFRGKLERADNGDWQVVWSDEPPAKPGQKVSRKRVPAPLQALG
                     FALDELKEARLAPIVDFKGRKSSALAGQATENRAVSTDQEVGES"
     gene            15849..17345
                     /gene="nusA"
                     /locus_tag="VGA59_17925"
     CDS             15849..17345
                     /gene="nusA"
                     /locus_tag="VGA59_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX9721600.1"
                     /translation="MNRELLMLVEAISREKNVERDVVFGAVEAALAQATKKLYEGDVD
                     IRVAVDRDTGDYETFRRWHVVPDEAGLQLPDQEILLFEAKEQIADIDVDDYIEESVDS
                     VPIGRIGAMAAKQVILQKIRDAEREMLLNDFMSRGDKIFVGTVKRMDKGDIIVESGRV
                     EGRLRRGEMIPKENLRNGDRVRAMIMEVDLTLRGAPIILSRAAPEFMIELFRQEVPEI
                     EQGLLEIKSCARDPGSRAKIAVLSHDKRVDPIGTCVGVRGTRVNAVTNELAGERVDIV
                     LWSEDPAQFVIGALAPANVSSIVVDEEKHAMDVVVDEENLAIAIGRGGQNVRLASDLT
                     GWKINIMDANESAQKHAEETDTIRKLFMEKLDVDQEIADILFAEGFTSLEEVAYVPIQ
                     EMLEIESFDEDTVNELRTRAKDALLTMEIAREESVEEVSQDLRDLEGLTPPLIAQLAD
                     AGVHTRDDLADLAVDELTDITGQSTDEAKTLIMKAREHWFTSATAAHE"
     gene            17369..20239
                     /gene="infB"
                     /locus_tag="VGA59_17930"
     CDS             17369..20239
                     /gene="infB"
                     /locus_tag="VGA59_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX9721601.1"
                     /translation="MSSTTVAEFATELKKSTSTLLEQLRSAGVAKSAATDVLSEADKQ
                     KLLGFLKASHGTAATPERRKITLVKKSTSEIKQADSTGKARTIQVEVRKKRTFVKRDE
                     GADTAVVEAPEPEVQQPAVHHIDDAELARREEEARRQADFIRRQEEELAEKRRQREQA
                     EAREREASEKAAAQQAEAIKEEVPAKAAAKAVVDEAATAVAKAQSDAREKAAVESKAR
                     ADEDVARAADLGERRRKAEAEAAAIRSMMSAPKKVLVPHKAPEPARPVAKAEAAKPGA
                     AKGTLHKPAVGTGTGRPPAAGAPATPGAPGSGKEVKSAKLSSSWAGDPAKKKAIPTRG
                     DSTGGVGRNSWRGGPRGRRSNERDHRDDHVQSAPVEARVLEVHVPETITVAELAHKMA
                     VKASEVIKHLMKLGQMVTINQPLDQDTAMIVVEEMGHKAVTAALDDPEAFTEQDVGAA
                     EGESLPRAPVVTVMGHVDHGKTSLLDYIRRAKVAAGEAGGITQHIGAYHVQTDRGMIS
                     FLDTPGHEAFTAMRARGAQATDIVILVVAADDGVMPQTKEAIKHAKAAGVPIVVAINK
                     IDKPDANTDRVKQELVAEQVVPEEYGGESPFVPVSAKTGEGVDALLEQVLLQAEVLEL
                     KAPVDAMAKGMVIEAELDKGRGPVATVLVQSGTLKTGDVVLAGQTYGRVRAMLDENGK
                     SIKSAGPSIPVEIQGLTEVPQAGDEFMVLTDERRAREIATYRAGKFRNTKLAKQQAAK
                     LENMFSEMTAGEVKMLPIIVKADVQGSQEALSQSLLKLSTDEVKVQLVYAAVGGISES
                     DVNLAIASKAVIIGFNTRADAGARKLAENNGIDIRYYNIIYDAVDELKAAMSGMLTPE
                     KREEVIGTAEIRQVFKVSKIGSIAGCMVTSGVVRRTARLRLLRDNVVVFTGELESLKR
                     FKDDVREVKEGFECGLNIKNYNDIQENDILEFFEIKEIARTL"
BASE COUNT         3730 a         6508 c         6592 g         3505 t
ORIGIN      
        1 gggtcgcggg ttcgagtccc gtccactccg ccaagaacat gttttttgca gtccaacaca
       61 gaccgcgaaa caccctcaga agcccctatc cacggggctt ttttgttttc tgacccgtgg
      121 ccgctgatcg gcagttcggc cggatgtcgt cagaacctgc tcttgcggca cgccggagat
      181 cagagtcgat acggacgtcg tgggcgagcg gtcgcgcaat tcctctcgtt caaagaccta
      241 tcgccgaagc gatgtccgac atgatcgacg ctacgtcgcg aggcagcgct tccctcgata
      301 gcctctcggc cctatgccga tcggcaaagt agtcggcgat cggattgaag ggcgccgtgg
      361 cgacgtcatt cgcaatgcgt ttggagatgc cgggattgtc ggcgacgaaa ctctggatca
      421 agccgaacgt ctgcgtcccg ctagtgaaga aaccccacgg ttgggccagc ttcggctcga
      481 gtgcgtcaaa actctggtgg ttacgccgca agccgctctt gatcttgttc gagcggtcgg
      541 aaatgtagat gttggtcaga aggacggcga cgaattcctc gctgtcgtcg tacttgttca
      601 gccccccgaa cacgggccgc ttggaccact tccccgagac gttgcggaac acgtggacca
      661 attcgtgaaa gaggacctcg tcccagtaca ggccgcggtt cttggccgat ttggtggccg
      721 agcacgcgcc gtgcaccgaa aacgtgtcgg gcgagtacca cacggcgccc tgtatcgcgc
      781 cgccgaccat ctgcccgccg ccaacggcat cgcatttgcc acccgtgtac ggctggatga
      841 cgaccggctt gccgtggaat ttgatgctcg ccagcaggat ccggccactg gtcgtccgct
      901 ggatccaggt gaggtgctcg gtgaccttgc gggtgtaggt ctgagcggcc gctccggcga
      961 ggccaaccgg attgaccgcg atgttgtatt gcgtgagaaa gttcatcact ccccctttcc
     1021 tcgggtatgt cgacggaaca cgtgcgaaca acgaccggca ttcccaacat caagaagctg
     1081 catgacatgt ggacagcaca gtcaacgggt gaaacggtgc cggcggacgg cacgaggctc
     1141 tcctggtgaa ggacagtact cgctgtccat ctccatgccg atcccgtggt tatgactgag
     1201 ggatgacaac aacgccgatg acgcaagggc cgacggcggg cggcaacccg cgatcagcat
     1261 caaagcggct tcggatggag cccggaaaaa acagctgaaa tttcttgacg tgggtcaata
     1321 tcccatggga aagccgtccc ctcgagctga cgcatcggcc gtccgctgct gcaatacacg
     1381 gcaaggcgcg cctgtttggt caccgccgcg ctgctgcgtc ccgagccgtc tctcttctgc
     1441 cggaggtccg tcatggcact cagtatttcc tcgccgatca acgtcctcgt gctcgacatc
     1501 tcgcagtccg tgggagtcaa gggaaaaaat cggtacggag acgtcatccg cgtgcaggag
     1561 gcgctcaaca cgatccccgc cagcgagggc acgtcatcca cattgctgtc gctcgacggt
     1621 atcgcggggc cgaaaacctg cgacgctatc cagaagctgc agttgaagca cttcggttgg
     1681 aaaggggcgg acggcaggat cgacccgggc ggcagcaccg cgcagctcat gtccggactg
     1741 ctggtgcgct atggcggcat tctctggacc atccgccggc tggagtccgc cgtcgctttg
     1801 aagagcaacc ttcaggatgt gcgcacgatc gaatcgaaag atcgcatctt cgagattgcc
     1861 gacgttaccg gcaggcgtcg ggcgctctac catttccgtc cggcagaagt gacgtacatt
     1921 ccgcgggcgg ccgacgtgcc ggcgctgcgc gagcgccccg aggtcaacat gttcaatacg
     1981 gttcttccgt gcggccccca cgccttcgct accgccgacg cgatgtattc ggagttctcc
     2041 ccggacgccg accatgcggt catccagctc acgttgcgac cccgtgcagc gcatatcgcg
     2101 ccaggtgtcc tcagcctgca cgtcaaccat cagtggatca agcccatgag caccccaggc
     2161 gccagagccc gcatggcggg catcttcggc ttcctgcgcg atgacagcgc ggtcggaggc
     2221 aagacaccca ggtggtgagt gaggcggttg ccttcgctcg gtgacgcgtc gccggcggct
     2281 acttcttgtc gcccggcgac cagtacttga ccgtcgaagc ctttttcagg gccgcgaccg
     2341 tctcctcgac ggtcaacaag gcagtggtct gcaaggaact cagggctccg ccggcgccga
     2401 tcgccagggc cgcggcggcc atcgagacat tgtccggagc ctcccacaac gtgtatccgt
     2461 cgtactcgcc gaacgcatac cagaaaccgt gcagctttcc gccgaccgat tcgatgtatt
     2521 gggaggccgc cgtgcgtcgg tcctcagggt tcttgatgag cttcgcccat gtcgctggcg
     2581 tgtagctgaa acgggtcagg tagaacggca tcgtcttctc cttcgcttca tcccgccggc
     2641 ggcgaacgcg cgccggaccg gggcgccagt atgcgcctcg tgacccgccg ccgcaatgtc
     2701 ccgcgccggg tgcgaactcg tctcggtctc cgcgtctagt aactgatgct cagcgacggc
     2761 acgtcgacat gcacaaccgg cttgccgagc gccccgctgg cggcacgcgc aaactcctgg
     2821 gcccatttgc cgtcggcgaa tttcgccggg cccgcggact gggccacgac caccaccttg
     2881 tccgccgtga gcaccttgcc cgtggcccgc aacggatcgc agtccagttc gatgaattgc
     2941 cgatcgagcc aggcgcgcgc ggcgtcgttc ggcatcggct cctccctgtt gagatccatt
     3001 cgcaccgggg cggcggcgcc gtcgaatgtc acggtaactt cacttcgcat gctggtagct
     3061 cctgttattt tgcagacgtg ggtattctcg atgttgcccg tatcgcggac aggccgcaag
     3121 ccgaaaaaag caggatcgcg ccggacgatg acggcgggtg tgggattggg gatggcgccg
     3181 ctagctcggt gtcgactgca ccggcgcacc aagatcgcgc atcctgatgg ccaggacctt
     3241 gatgcgctcg gcggcggccg cccaccgcgc gtaggccttg ccacgctcga cggccccgag
     3301 ctcggtgtcg ctcagggcct tccacgcgga gcgttcctgc gccgtggcag ccctgtactg
     3361 cgcctgcagc gattcgtatg gatcagcgtg tacagccata gaggctctaa ttctcacgcc
     3421 gctccatgtc acggtcgagg tattttccgg tgacttattt gacaacggcg gcgcctgcga
     3481 cgcgcgccga aacgacagcg gctgcctgcc gctgaagccc acattcacgg agtaccggag
     3541 tcttgcgctg ctaatggcgc ggtcgaattg tcaggccctg gcgtgaattc ggcgagcggc
     3601 cggcgccggt tgaacccttt gcctcgatga attccgagtt gaaagcctca agctccgagt
     3661 cgctctcctg ctgcaggcga ctgacttccg cctccaggca agcccgtgtc gagtccgcag
     3721 gcgtggcctg cagttgtttc aagcgctggc gcgcatcctg cagttgcaga tacaggcggt
     3781 gccaggcgga taacttcccc tcgaccttgt tcacgctcac acctccaccg aggatgaacc
     3841 gcgcgttgac aaatgcaacg cgaccgtcac acatttttag ctgctagttt gtcctggatc
     3901 aaacagggtt ttccctgcct cagccgtaaa gattcgcgcc cggtcggggg acagaccgcc
     3961 cgggggcggg cgggtcaggc ggccgccttg ccggtctgca ccatccgcat cgccgaagcg
     4021 atgagggccg acacctcggt catgttgctt ggcacgatca atgtggtggt ggagtccgta
     4081 gcgaccttgc tgtaggcttc cacagccttt tccgccactt tgagctgcac cgcctgctcg
     4141 ccgccgggct ggcggatcgc ggcggcgatg cgctcgatgg ccattgccgt ggcatcggcc
     4201 accgcggtga tcgaagcggc ttcgccctgg gcgttgttga tgatcgactg tttttcgccc
     4261 tcggaacgcg cgatgaaggc ctcgcgctcg ccggtggcga tgttgatctg ctcctggcgc
     4321 cggccttcgg aggcggcgat cagcgcgcgc ttttcgcgct ccgcggtgat ctgcgattgc
     4381 atggcatgca ggatttcctt gggcggcgtc aggtccttga tctcgtagcg caggactttt
     4441 acgccccagt tcagcgccgc ttcgtcgatg gcctgcacca cctgggcatt gatgatgtcc
     4501 cgctcctcga aggtcttgtc gagttccagc ttgccgatca cgctgcgcag cgaggtctgg
     4561 gcgagctgcg tcaccgccat gatgtagttg gacgaaccgt agctggcgcg catgggatcg
     4621 gtcacctgga agtacaggat gccgtcgacc tgcagctggg tgttgtcgcg cgtgatgcag
     4681 acctggctgg gcacgtccag cggaatttcc ttcaggctgt gcttgtaggc cacgcggtcg
     4741 atgaagggaa ccaggaaatt caggcccggt gccagcgcgg cgtggtattt gccgagccgc
     4801 tccaccaccc aggcgttctg ctgcggcacg accttgatcg aacgggtgat aaagatgacg
     4861 gcgatgacca ggatgacgat cgcgatttcc atagagagcc tttttggttg gttatgaaat
     4921 cttcagatgg gatcgaccag caggcggctg ccgatcagtt cggcaacgcg gtgcggtcca
     4981 gccacaggta tctcgccctc gcggtgcacg gccgtccagg tggcgccccg gtacttgacg
     5041 gtggccgtgc cgtcgggatt ccagcgctcg atgtgaaccg tcgagccgac gtccagattg
     5101 acgctgcgat cggcgcgaac cgacgggtcc tccggccgcc tcttcttggc gtagtaccag
     5161 cccacgacgg aactgccgcc gaccacggcc gcggcaagca gctgcgtcgc cgttccggcg
     5221 ccgatgtggg ccgccagcgc cccggccgcc agcccgatgg ccagcatcag caggtaaaag
     5281 gttcccgtga ccagttccac cgcgatggcc gtcccggcca tcagccacca gatggtcgat
     5341 tcagccattt cgggcctccg ccgggaggcc ccaggcaggc gctcggcctc ccgcaagccg
     5401 gtacagcggc gtggccgcca gagtcggggt cgtcatctct tcctccaaac ggtttcgtgg
     5461 tgtgtcttga tggcaccaca ttttgggtgt attcgaacat tgcgcaagaa gggcgttgcc
     5521 ttatttgcat acacttcgcg cctgttttcg ctttttttct ggcccgcggg ctggaggccc
     5581 catgaaattc cgctttccca tcgtcatcat cgacgaagac ttccgctcgg agaatacctc
     5641 cggcctgggc atccgcgcgt tggcgcaggc aatcgaaacc gagggcttcg aggtgctggg
     5701 cgtcacccgc tatggcgacc tgtcgcagtt cgcccagcag caaagccgcg ccagcgcgtt
     5761 catcctgtcg atcgacgacg aggagttcac gcccgggccc gacctggacc cggcggtggt
     5821 gaacctgcgc agcttcatcg aggaggtgcg gcgcaagaac gcggacgtgc cgatctacgt
     5881 ctatggcgag accaagacct cgcgccacat ccccaacgac atcctgcgcg agctgcacgg
     5941 cttcatccac atgttcgagg acaccccgga gttcgtggcg cgccacatca tccgcgaagc
     6001 caagagctac ctcgaggggg tgcagccgcc gtttttcaag gcgctgctcg attacgccga
     6061 ggatggctcc tactcctggc actgccccgg ccactcgggc ggagtggcgt tcctgaaaag
     6121 cccggtgggc cagatgttcc accagttttt cggcgagaac atgctgcgcg ccgacgtctg
     6181 caacgcggtg gacgaactcg gccagctgct ggaccacacc ggcccggtgg ccgccagcga
     6241 gcgcaacgcg gcgcgcattt tcaacgccga tcattgcttt ttcgtcacca acggcaccag
     6301 cacctccaac aagatcgtgt ggcaccacac cgtcgcgccg ggcgacgtcg tcgtggtgga
     6361 ccgcaactgc cacaagtccg tcctgcacgc gatcatcatg accggggcca ttcccgtgtt
     6421 catgaagccc acgcgcaatc atttcggcat catcggcccg atcccgcaaa gcgagttcga
     6481 gcccgccgcg atccgggcca agatcagggc caacccgctg ctcaaaggcg tggacccgga
     6541 cagcgtcaag ccgcgggtga tgacgctgac ccagtccacc tacgacggcg tcatctacaa
     6601 caccgagacc atcaagaaga tgctcgacgg ctacgtggac acgctgcact tcgacgaggc
     6661 ctggctgccg cacgccgcct tccatccctt ctacgggccg taccacgcga tgggcaagaa
     6721 ccgcgtgcgg cccagggagt cgctgacctt cgcgacccag tccacccaca aattgctggc
     6781 cggcatcagc caggccagcc atgtgctggt gcaggactcg cagaaccgca agctcgaccg
     6841 gcacctgttc aacgaggctt acctgatgca cacctccacc agcccgcagt acgcgatcat
     6901 cgccagctgc gatgtggcgg cggcgatgat ggagccaccc ggcggcacgg cgctggtgga
     6961 ggaatcgatc ctggaggcgc tggatttccg ccgcgccatg cgcaaggtgg acgaggagta
     7021 cggcaaggac tggtggttca aggtctgggg cccggacaag ctggtggagg agggcatcgg
     7081 ccgcgccgac gactggatca tcaagggcga ggcgcgcacc gccaagtggc acggcttcgg
     7141 caacctggcc gagggcttca acatgctgga cccgatcaag tccaccatcg tcactcccgg
     7201 catggacctg aacggaaagt tcgccaagac cggcattccg gccagcatcg tgacccgctt
     7261 cctggcagaa cacggcatca tcgtggagaa gaccggcttg tacagcttct tcatcatgtt
     7321 caccatcggc atcaccaaag gccgatggaa cactatgctg acggcgctgc agcagttcaa
     7381 ggacgactac gccaagaacc agccgatgtg gcgcatcctg cccgagttct gccagcagtt
     7441 cccgcgctac gagcgcatgg gactggccga cctgtgccag cacatccacg agctctacgc
     7501 caagtacgac atcgcccggc tcaccaccga catgtacctg agcgacctga cgccggcgat
     7561 gaagcccagc gacgcttatg cattcatcgc ccaccgcaag accgagcggg tggcgatcga
     7621 cgagctcgaa ggccgcatca cgacctcact ggtgacgccc tatcccccgg gcatcccctt
     7681 gctgatcccg ggcgaggtgt tcaaccggaa gatcgtggat tacctgaagt tctcgcgcga
     7741 gttcaatgcg cagtgcccgg gcttcgagac cgacatccac ggcctggtcg aagaagccgg
     7801 gccggacggc gcgctgcgct actacgcgga ttgcgtgcaa aacggcgacc gcgcgtgagg
     7861 cgctcccggg cgggggcagc acttctgtca ttgcggcctc gagccgcaat ccatgacccc
     7921 gcattcatgg atgcccccgg atcagagcct gccccggact cgatccgggg tccggggcag
     7981 gctcgtcaag ccgggcatga caactgtcat caaatctctg gacagcagtg ggtcaagccc
     8041 gtaatgacaa agtccggtct caggtttcga caacggccac ggcggtctgg ctgaagccgg
     8101 cgtcaacgta ggtgatttcc gccgtgacgc cggatgccag gtcgcttagc agaaacgccg
     8161 ccacgttgcc gacgtcctcg atggtgacgt tacgccgcag cggcgacgca ctggcgcaga
     8221 tgctcagcag cttgccgaaa tccttgatgc cgctggcggc caatgtcctg atgggccccg
     8281 cgctgatgcc gttggcccgc atgctgcggc catccggcag ttgccccacc gcttcggcca
     8341 ggtaacgcac cgacgcttcg aggctggctt tggccaggcc catggtgttg tagttgggga
     8401 tcgcccgcat ggcaccgaga taggtgagcg tcaggatggc ggacttgtcg ttgaggtagg
     8461 gcagggcggc cttggccatc gcgggaaagc tgtaggcgct gatgtccagg gcgatgcgga
     8521 agttctcgcg ggtcaggccc tccaggaaac tgcccgagat cgcctcgcgc ggcgcgtagc
     8581 cgatgctgtg gacaaagccg tcgaacgtgg gccacgcgtt cgacagatcc ttgaacagcc
     8641 ggtcgatctg cgcatcgtcg ccgacatcgc agtcgaagat cagctgggaa tcgaagtcgg
     8701 ccgcgaactc ggcgatgcgt tccttgaagc gctcgcccac gtagctgaag gccagttccg
     8761 ccccctgcgc gcgacaggcc ttggccaccc cataggcgat ggagcggttc gacagcacgc
     8821 cggtaatcaa caatttcttg ccagccagga atcccataga tctctttagt tgataaattc
     8881 tgtgcagaat tgtcgcatgc gaggattgct cttccttctg gcctgcctgg tggcgggtcc
     8941 aagctcggcc gggtacgcct atgcccagtt cggcgacatc aagtacccgc cgggcttcac
     9001 gcacttcgat tacgtgaacc cgaacgctcc caaaggcggt gaaatccgca tggtgccgcc
     9061 cacgcggccg agcaatttcg acaagttcaa ccctttcacg ctgaagggga acgcgcccta
     9121 cgacatcgac ttgttgttcg acagcctgct ggtgggaaat tccgacgagc cgaccacggc
     9181 ctacggcctg ctggcggaag acgtggaggt cgcgccggac cggctgtcgg ccaccttcag
     9241 gctcaacccc aaggcgcgtt tccatgatgg caagccggtg ctggccgccg acgtggtgca
     9301 ttctttcaag accctcatca gcgaggaagc ggcgccgcag ttccggacga tctacgcgga
     9361 agtcaaggat gcgacggccg tggccgagcg cacggtgcgc ttcgatttct ccagccccaa
     9421 ccctgaactg ccgctggtcg tgggcggcat gtccgtgttc agccgcgagt ggggcaaggg
     9481 caagccgttc gacgaagtgg tgtccgagat tcccatcggc tcgggccctt accggatcgc
     9541 caacccacgg atggggcgcg acatcaccta cgtgcgcgat cccaactact gggccgccga
     9601 cctgcccacg cgcaaggggc agttcaactt cgaccgcatc acgttcaaga tctacctgga
     9661 cgagacctcg cgtttcgaag gcctgaaagc cggcgaattc gacttcatgc gcgaattcat
     9721 ctcgcgcaac tgggcgcggc agtacaaggg caagcacttc aactccggcg aactggccaa
     9781 gcgggccttc gagaaccgca acccgggcga cttccagggc tatatcttca acttgcgcaa
     9841 gcccaagttc caggacatca gggtgcgcaa ggcgatcgga ctcgccatgg atttcgaatg
     9901 gatgaacaag cagctgttct acggcatcta caagcgcgtg aacggctatt tccccaacag
     9961 cgacttccac gccgaagggc tgcccaaacc ggacgagctg gctctgctgg aaccgctgcg
    10021 cgacaagctg cggcccgaag tgttcggacc cgcgcccgtt tcgcacagca ccgatccgcc
    10081 cgggagcctg cgcgagaacc tgcgccaggc ccaggccctg ttcaaggagg ccggctggga
    10141 ataccgcgac ggcgcgctgc gtaacgccaa cggcgaagcc ttcaccatgg aatttctcaa
    10201 cgaccagccg tcgctgatcc gcatcgtcgg gccattccag aaagccctgg aaaagctcgg
    10261 cgtccagctg atctaccggg tggtcgactt ctccctgtcc aaacagaaga tggacgcctt
    10321 cgatttcgag gtcaccacct tgcggctgcc ggggcgaacg gcccccggcg gcgaactgct
    10381 ggagcttttc ggctcgaagg cggccgccac cccgggctct tccaacgtgt ggggcatcgc
    10441 cgatccggcg gtggacgcgc tgctgcagaa agtggtcacc gcgcgcaccc gcccggatct
    10501 cggtgcggcc atgcgggcac tggaccgggt cctgtcgcac ggctactact cggtgccgca
    10561 gtactacggt gacgccttcc tggtcggcta ccggccccgc ccgttcgtgt tgcctcccag
    10621 gattccgccg tactaccagg ttgacagctg ggccctttcc acgtggtggg catcgcccga
    10681 gaaccggtga ggccgggatg acaagctaca tcctcaagcg cctgctgctg atgatcccga
    10741 cgctgttcgg gatcctgctc gtgactttcg cgatcgtgca gttcgtgccg ggcgggccgg
    10801 tcgaacagat ggtggcgcag ctccagggcc gcgacacggg aggcgagcgg gcggcggctg
    10861 ccggcgccgg gtaccgcggg cggcagggcg tggatgaaaa aaggatcgag gagatcagga
    10921 agctctatgg cttcgacaag ccggcccacg agcgcttcct gcagatgctg ggtcagttcg
    10981 cccggttcga ccttggccag agcttctacc agcacaagga cgtgtgggaa ctggtcaagg
    11041 aaaagatgcc ggtttcgatc agcctgggcc tgtggacgtt cttcctgagc tacctgatag
    11101 ccgtgccgct gggcgtcgcc aaggccgtgc gtgccggctc gcgcttcgac ttcgtcacaa
    11161 cgctggtcgt cctggtgggc tatgcgattc ccggcttcgt gctcggagtg gcgctgctgg
    11221 tgatcttcgg cgggcagctg cagtggtttc cgctgcgcgg gctgacctcg tccaactggg
    11281 agcaactggg ctggggcgcc cgcatcgtcg actatctctg gcacatcgcc ttgcccgtca
    11341 cggcgatggt tttcggcagc ttcgcggtca cggcgatgct gaccaagaat tcgttcctgg
    11401 aggaaatccg caaacagtat gtgctgacgg cgcgcgccaa gggactggac gagcgcaagg
    11461 tcctgtggaa gcacgtgttc cgcaacgccc tgatccccat catcacgggc ttcccggcgg
    11521 ctttcatcgg cgcattcttc accggctcgc tgctgatcga gaccctgttc tccctggacg
    11581 gcctcggact cctgagctac gagagcgtga tccggcgcga ctacccggtg gtgctgggca
    11641 cgctctattt cttcaccctg atcggcctgg tgaccaagct gatcagcgat ctttcctacg
    11701 tgctggtgga cccgcgtgtc aagtttgact aacccccccg aagcggcctc cggccgcctc
    11761 ccccccgggg gggcgccacc agcggccggg caaagcccga tccgcggtgg ccgctggaac
    11821 gattccctgc ccgcggcggc ggccgagccg gcaagcgcgc cgccggcggc gttcgtcagc
    11881 cccgggcggc gggcctggca gcgctttcgg cgcaacaagc tgggcttcta cagcctcgtg
    11941 attttcagcg tgctggtggt gctgagcctg ttcgccgagg ttctctccaa tgacacgccg
    12001 ctggtcgttc gctacgaagg ccaatggtat ttcccgatcg ccaaggacta tccggaaacc
    12061 accttcggcg gcgacttcca tacgcccacc gattacctcg accctttcgt ccgcgacaag
    12121 ctgacgcagg gcggcaactg ggccttgtac ccgcccaatc actatggtcc caagaccctg
    12181 aactatttcg ccagccagcc caatcccacg gcgccttcgg gcgagaacat cttcggcacc
    12241 gatgaccgcg gccgggacct gctcgcccag ctgatctacg gtttcagggt cagtgtgctg
    12301 ttcgggctgg cgctcaccgt ggtcggggtg gtcatcggcg tgatcaccgg cgccgtccag
    12361 ggatatttcg gtggcaagac cgacctggcc tttcagcgct tcatcgagat ctggggctcg
    12421 atgcccgagc tctacctgct gatcatcttc agcgcggtgt tcgcgcgcag cgtcgggctg
    12481 ttgctgatcc tgctgtcgct gttcggctgg atggggctat ccgattacgt gcgcgccgag
    12541 ttcctgcgca accgccagat ggactatgtg cgggcagcca aggcattggg ggtgggcaac
    12601 ggccgcatca tgtggaagca catcctgccc aacagcatga cgccggtggt gacgttcctg
    12661 ccgtttcgca tgagcgccgc catcctggcg ctgacctcgc tggactttct cgggctgggc
    12721 gtgccgccgg gcacgccttc gcttggggaa ttgctgaacc agggcaaggc caacctggac
    12781 gcctggtgga tttcgctgtc gaccttcggg gtgctggtcc tcacgctgct tctgctgacc
    12841 ttcatgggcg atgccttgcg cgacgcgctc gatccgcgaa aggccgagaa atgacgaccc
    12901 ccacgcttgc ggctggagcc gctgcgctgc cccctgaggg ggccttcgcg tccaggggcg
    12961 ggccgtcgcc gctcaagttg ctgctcgacg tcaagggcct gcgcgtcgcc ttcggagaaa
    13021 aggaggtcgt gcgcggcgtg gacttttcga tcgctcccgg cgaaaagctg gcgctggtgg
    13081 gcgagtcggg ctctggcaag acagtcacgg cgctgagcct gctggggctg gtccagaacg
    13141 cacgggtctc gggttcggcc atgttcgccg gtttggcgga cccggcgaac ccgcgagacc
    13201 tgctgtccat gtcggagcgc gagctgctgg cgacgcgggg ccgggagatc gcgatgatct
    13261 tccaggagcc gatgaccgcg ctcaaccccc tgtacaccgt gggcgaccag gtcgccgagg
    13321 tgctgcagct caagcaggga ttgtcggggc gcgaggcctg ggaggcggcg gtcggggccc
    13381 tggccgctac cggcatcccc gagccggagc gccgcgcctt cgcctatccg caccagcttt
    13441 cgggaggcca gcgccagcgg gcgatgatcg ccatggcgct gtccggacga cccaagctgc
    13501 tgctggccga tgaaccgacg acggcgctgg atgtcacgct gcgtgcccag atcctcgact
    13561 tgctggccga ggtgcaacac caaaacggca tggcggtact gctgatcacg catgacctga
    13621 acctggtgcg caagttcgcc gaccgcgttg ccgtcatgga gaacggctac ctcgtggagc
    13681 acgggaccgt cgacgaggtt ttcgccaacc cgcggcacgc gtacacgcgc aagctcattg
    13741 agagccgccc ggtgcgcgac gtgaccgagg cgccggcacc gccggagccg ccggtcatga
    13801 gcgcgagcgg cgtggaggtg agctatccgg tcccgatcgc gggctttcgg ggctggttca
    13861 ggaagggccg gttcgtcgct gtgcgcggcg cgggcttttc cattgcacct gggcgaacat
    13921 tgggtgtggt cggcgaatcc gggtccggca agtcgaccct ggcgttggcg gctttggggc
    13981 tgatgcccca cagcggcgag ctggaggtcg tgggccgccg ctggggcccg gactcggcgg
    14041 gcaacaaggc aatccggcgg ttggtgcaag tcgtgtttca ggatccctat tcatccctgt
    14101 cgccgcgcat gacggtggaa gagatcgtgg gcgagggcct gctggtgcac gagccgggcc
    14161 tgcccgaggc cggacggcgc gagcgggtga tccaggcgct cggcgaggtc gggctgtcgg
    14221 aggagcagtt ccccgggctg cttcagcgct acccgcatga gttctccgga ggccagcgcc
    14281 agcggctggc gatcgcccgg gcgttgatcg tccagccgcg gctgctcatc ctggacgagc
    14341 ctaccagcgc gcttgatgtg acgatccaga aacaggttct caaattgctt cagcggctgc
    14401 agcgcgagaa aaggctgagc tatatgctca tcacccacga cgtggacgtg attcgcgcaa
    14461 tggcgcacga cgtggtggtg atgaaggatg gggacatcct ggagtcgggg acggtcaccc
    14521 aggtgctcga cgcgccgcgg caggactaca cccgcatcct ggtggcggcg gcctcttgaa
    14581 atgaaagcgc gaccccccac cgccatcccg gagtcggaag tggaaatcac ggccgtgcgg
    14641 gcccagggcg ccggcggcca gaacgtcaac aaggtgtcca gcgcgatcca cctgcgtttc
    14701 gacattccca actcatccct ggcgccggat cacaaggagc ggctgctcgc gctggccgac
    14761 caacgcataa cccgtgatgg ggtactggtc atcaaggcgc agtcgcatcg aagccaggac
    14821 atgaaccggg ctgaagcttt ggggcggctg caggaactgg tggacagcgt agccgcaccg
    14881 ccccggcctc gccggccgac caagccgacc ttgggctcca agaaacggcg cctggaggcc
    14941 aagaacctgc gctctcaggt caagctgctt cgcagccggg tctccgagta gccccaccgg
    15001 cgtcggggtg cccgaaacgg tgcccgtaac gttgcaaatc aagggtttgc gaggtacaat
    15061 ccttgcttca cgaccaaacg ggcgggtact caccgcccgt tttttttgcc tgtgcataac
    15121 aagcgttcgc gctctgcgct tcacgcattg tcagcacagg cgcaaccttt ggttgaaggc
    15181 ctgtgagcag ttggcatgga aaggtcgttt ttcttggggc atcgcacaga cagtggcact
    15241 gcaggagatc gtagaacaaa ccgtaaccgg gctcgggtac gaactggtgg agattgagcg
    15301 ctccgccggc ggactgctgc gcatcacgat cgacctgccg tggagccctg gcgtcgagcg
    15361 gtttgtcaac gtggaagact gcgagaaggt cacgcgacag ttgcagttcg cgctggaggt
    15421 cgagggcgcc gagtacaagc ggctggaagt gtcgtcgccg ggcatagacc ggccgttgcg
    15481 tactgaaaag gatttcgagc gttttgccgg cgagctggtc gacatcacgc tcaaggcgcc
    15541 tatgggcgcg gcggccggtg gccaggtcgc ggccaaccgc aagaagtttc gcggcaagct
    15601 ggagcgcgct gacaacggtg actggcaagt cgtctggagc gacgagccgc ccgcgaagcc
    15661 gggccagaag gtgagcagga aaagggtgcc ggcgccgttg caggcgctgg gttttgcgct
    15721 ggacgagctg aaggaagcgc gcctggcacc catcgtggat ttcaaggggc gcaagtccag
    15781 cgccctggcg ggacaagcca cggagaaccg tgccgtgtca actgatcaag aagtaggaga
    15841 gtcgtgagat gaatcgcgaa ttgttgatgc tggtggaagc catttcgcgc gagaagaacg
    15901 tcgagcgcga cgttgtgttc ggcgccgtcg aggcggcctt ggcacaggcc acgaaaaagc
    15961 tttacgaagg cgacgtggac atccgcgtgg cggtggaccg cgataccggc gactatgaaa
    16021 cattccgtcg ttggcacgtc gtgccggacg aggcgggcct gcagctgccg gaccaggaga
    16081 tcctgctgtt cgaagccaag gagcagattg ccgacatcga cgtggacgac tacatcgaag
    16141 agtcggtgga ctcggtgccg atcgggcgca tcggggccat ggcggccaag caggtgatcc
    16201 tgcagaagat ccgggacgcc gagcgcgaga tgctgctgaa cgacttcatg tcgcgcggcg
    16261 acaagatctt cgtgggcacc gtcaagcgca tggacaaggg cgacatcatc gtcgagagcg
    16321 gccgcgtcga gggccgattg cgccgcggcg agatgattcc caaggagaac ctgcgcaacg
    16381 gcgaccgcgt ccgagccatg atcatggaag tggacctgac gctgcgcggc gcgcccatca
    16441 tcctgtcgcg cgcggcgccc gaattcatga tcgagctgtt ccgccaggaa gtccccgaga
    16501 tcgagcaggg cctgctggag atcaagagct gcgcccgcga ccccgggtcg cgcgccaaga
    16561 tcgccgtgct gtcccacgac aagcgggtcg accccatcgg cacctgcgtc ggcgtgcgcg
    16621 gcacgcgcgt gaacgccgtg accaatgagc tggccggcga gcgcgtggac atcgtgctgt
    16681 ggagcgagga tccggcccag ttcgtgatcg gcgccctggc cccggccaac gtgtcgtcca
    16741 tcgtcgtcga cgaggaaaag cacgcgatgg acgtggtggt ggacgaggaa aacctcgcga
    16801 tcgcgatcgg ccggggcggt cagaacgtgc gcctggcgtc cgacctcacc ggctggaaga
    16861 tcaacatcat ggatgcgaac gaatcggccc agaagcacgc cgaggaaacg gacacgatcc
    16921 gcaagctgtt catggaaaag ctggatgtgg accaggaaat cgccgacatc ttgtttgccg
    16981 agggcttcac cagcctggaa gaagtggcct atgtgccgat ccaggagatg ctggagatcg
    17041 aatcgttcga cgaggacacc gtcaacgagc tgcgtacgcg cgccaaggat gcgctgctga
    17101 cgatggaaat cgcccgcgag gaaagcgtcg aggaggtctc tcaggacctg cgcgacctgg
    17161 aaggcctcac gccgccgctg atcgcccagc tcgccgatgc cggcgtccac acccgcgatg
    17221 acctggccga cctggccgtc gacgaactta ccgatatcac cggccagtcc acggacgagg
    17281 ccaagaccct gatcatgaaa gcgcgcgagc actggttcac cagcgccacc gccgctcacg
    17341 agtaatgtga tggaaggcac cacaacacat gtccagtacc accgtcgccg agtttgctac
    17401 cgaactcaag aaatcaacca gtacgctgct tgagcagctt cgcagcgcgg gcgtggccaa
    17461 atcggccgcg accgacgtgc tcagcgaagc cgacaagcag aagttgttgg gcttcctcaa
    17521 ggccagccat ggcaccgccg ccacgcccga gcgcaggaag atcaccctgg tgaagaagtc
    17581 caccagcgag atcaagcagg ccgattccac gggcaaggcg cgcaccatcc aggtcgaggt
    17641 gcgcaagaag cgtactttcg tcaagcgcga tgaaggcgcc gatacggccg ttgtggaagc
    17701 gcccgaaccc gaggtccagc agccggccgt ccaccatatc gacgacgcgg aactggctcg
    17761 ccgtgaggaa gaggcacgcc gccaggccga cttcatccgc cgccaggagg aagagctggc
    17821 cgagaagcgc cgccagcgtg agcaggccga ggcgcgcgag cgcgaagcct ccgaaaaggc
    17881 cgcggcccag caggccgagg ccatcaagga ggaagtgccg gccaaagcgg cggccaaggc
    17941 cgtggtcgat gaagcggcca ccgcggtggc gaaagcgcag tccgacgccc gcgagaaggc
    18001 ggctgtcgaa tcgaaggccc gcgccgacga ggacgtggcg cgcgcggccg acctgggcga
    18061 gcgccggcgc aaggccgagg ccgaagccgc ggccatccgt tccatgatgt ccgcgcccaa
    18121 gaaggtgttg gtgcctcaca aggcgccgga gcccgccagg ccggtggcca aggccgaagc
    18181 cgccaagccc ggcgccgcca agggcacgct gcacaaaccg gctgtcggca caggcacggg
    18241 ccggccgccg gccgccggcg ccccggcaac ccccggagcc cccggctcgg gcaaggaagt
    18301 gaagtcggcc aagctgtctt ccagctgggc gggcgatccg gccaagaaga aggcgattcc
    18361 gacccgcggc gacagcacgg gcggtgtcgg ccgcaacagc tggcgcggtg ggccacgggg
    18421 ccgccgcagc aatgagcgcg accatcgcga cgaccacgtg caaagcgcgc cggtcgaggc
    18481 gcgcgtgctc gaagtgcacg tgcccgaaac gatcacggtg gccgaactcg cgcacaagat
    18541 ggccgtgaag gcctccgagg tgatcaagca cctgatgaag ctgggacaga tggtcacgat
    18601 caaccagccg ctggaccagg acacggccat gatcgtggtc gaggaaatgg ggcacaaggc
    18661 cgtgacggcc gcgctggacg atccggaagc gttcaccgag caggatgtgg gcgcggccga
    18721 gggcgaatcg ctcccgcgcg caccggtggt caccgtcatg ggccacgtcg accacggcaa
    18781 gacgtccttg ctggactaca tccgccgcgc caaggtggcg gcgggcgaag ccggcggaat
    18841 cacgcagcac atcggcgcct accacgttca aaccgaccgc ggaatgatct cgttcctgga
    18901 cacaccgggc cacgaggctt tcaccgccat gcgggcccgc ggagctcagg caaccgacat
    18961 cgtgatcctg gtggtcgccg ccgacgacgg cgtgatgccg caaaccaagg aagccatcaa
    19021 gcacgcgaag gcggccggcg tgccgatcgt ggtggcgatc aacaagatcg acaagcccga
    19081 cgccaacacg gaccgcgtca agcaggagct ggtggccgag caagtcgtcc ccgaggagta
    19141 cggcggcgaa tcgccgttcg tgcccgtgtc ggccaagacc ggcgaaggcg tcgatgcgct
    19201 cctggagcag gtactgctgc aggccgaagt gctggaactc aaggcaccgg tcgatgccat
    19261 ggccaagggc atggtgatcg aagcggaact ggacaagggc cgcggcccgg tggcgaccgt
    19321 gctggtgcaa tccggcacac tcaagaccgg tgacgtcgtt ctggccggcc agacttatgg
    19381 ccgtgttcgc gccatgctgg acgagaacgg caagtcgatc aagtcggccg ggccttcgat
    19441 tccggtggaa atccagggcc tgacggaagt cccgcaggcc ggcgatgagt tcatggtgct
    19501 caccgacgaa cgccgtgcgc gcgagatcgc cacgtaccgt gccggcaagt tccgcaatac
    19561 caagctggcc aagcaacagg ccgccaagct ggagaacatg ttctccgaga tgactgccgg
    19621 cgaagtcaag atgctgccga tcatcgtcaa ggcggacgtg caaggctcgc aggaagcgct
    19681 gtcgcaatcg ctgctcaagc tgtcgaccga cgaggtgaag gtgcagctgg tatacgccgc
    19741 cgtaggcggc atcagcgaat ccgacgtgaa cctggccatc gcgtccaagg cggtgatcat
    19801 cggcttcaac acccgcgccg acgccggcgc gcgcaagctg gccgagaaca acggcatcga
    19861 catccgctac tacaacatca tctacgatgc cgtcgatgag ttgaaggcgg cgatgtcggg
    19921 catgttgacg cccgagaaaa gggaagaggt catcggcacg gccgaaatcc gccaggtgtt
    19981 caaggtcagc aagatcggtt ccatcgccgg ttgcatggtc acctccggcg tggtgcgtcg
    20041 cacggcgcgc ctgcggctgc tgcgcgacaa cgtggtcgtc ttcaccggcg aactggagtc
    20101 gctcaagcgc ttcaaggacg acgtgcgcga ggtcaaggaa ggcttcgagt gcggcttgaa
    20161 catcaagaac tacaacgaca tccaggagaa cgacatcctg gagttcttcg agatcaagga
    20221 aattgctcgg acgctgtaat ttcaaacgcg gacatccggc cgcgaaggac gcaaagattt
    20281 cgcgaagaac gcgaaagaat tcaagaattt gcttttttgt cttttttgcg acctt
//
LOCUS       DASWIY010000499         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_735247, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000499 DASWIY010000000
VERSION     DASWIY010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735247"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VGA59_17935"
     CDS             complement(<1..995)
                     /locus_tag="VGA59_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9721602.1"
                     /translation="MLLTQDQEMIRDAVRAFAREQLWPNAPRWDKEHYFPHEAHRGLA
                     ALGAYGICVPEEYGGADLDYVSLALVLEEIAAGDGGTSTAISVTNCPVNAILMRYGSD
                     AQKKRWLAPLARGEMLGVFCLTEPHVGSDASALRASAVREGDSYVLNGVKQFITSGKN
                     GQLAVVIAVTDKGAGKKGMSAFLVPTDTPGYVVARLEDKIGQHSSDTAQINFDNCRIP
                     ADHLIGDEGQGYRIALSALEGGRIGIAAQSVGMARSAFEVAVDYAKERQSFGTAIINH
                     QAVGFRLADCATQLEAARQLIWHAASMRDAGQPCLKEAAMAKLFASEMAEQVCSAA"
     gene            complement(1021..1689)
                     /locus_tag="VGA59_17940"
     CDS             complement(1021..1689)
                     /locus_tag="VGA59_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9721603.1"
                     /translation="MSTVLVIGASRGIGLEFVRQYRGQWDRVFATARDDAGLGRIEGL
                     GARAHRLDVTDPAGAGALRAQLEGEKLDIAIYVAGVYARGDATAQPLPQEFDRVMHTN
                     VLGAMQALVQIAPLVEAAEGRFAFVTSEMGRVGGVASSHGWTYRVSKAALNMAVAAAQ
                     FDYPRATLVAINPGWVRTDLGGTGAPLDVERSVAAMRLTLAGLTRRHKGAFLNYDGRR
                     YKTW"
     gene            complement(1921..>2388)
                     /locus_tag="VGA59_17945"
     CDS             complement(1921..>2388)
                     /locus_tag="VGA59_17945"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704029.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9721604.1"
                     /translation="DGTITAASSSSINDGAAAMVLMKESTAVRYGSKPIARIVGHAMH
                     SQEPEWFTTAPVGAVQKLLAKTGWKAGDVDLWEVNEAFAVVPMALMKELGVSHEVLNV
                     NGGACALGHPIGASGARIMVTLIHALKARGGKRGIATLCIGGGEGTAVALELL"
BASE COUNT          334 a          863 c          800 g          391 t
ORIGIN      
        1 gccgcgctgc agacctgttc ggccatttcg ctggcgaaga gcttggccat ggccgcttcc
       61 ttcaggcagg gctggccggc gtcgcgcatg ctcgccgcgt gccagatcag ctggcgcgcc
      121 gcttcgagct gggtcgcgca gtcggccagc cggaagccca ccgcctggtg gttgatgatg
      181 gcggtgccga agctctggcg ctccttcgcg taatccaccg ccacttcgaa ggcgctgcgc
      241 gccatgccca cgctctgggc cgcgatgccg atgcgcccgc cctcaagcgc cgacagggcg
      301 atccggtagc cctgcccctc gtcgccgatc aggtggtcgg ccgggatgcg gcagttgtcg
      361 aaattgatct gcgcggtgtc gctggaatgc tggccgatct tgtcctccag ccgcgcgacc
      421 acatagccgg gcgtatcggt cggcaccagg aacgcgctca tgcccttctt gcccgcgccc
      481 ttgtccgtga cggcgatcac gaccgccagc tgcccgttct tgccgctggt gatgaactgc
      541 ttgacgccat tgagcacgta gctgtcgccc tcgcgcacgg cgctggcgcg cagcgcggaa
      601 gcatcgcttc ccacatgcgg ttcggtcagg cagaacacgc ccagcatttc gccgcgcgcc
      661 agcggtgcca gccagcgctt cttttgcgcg tcgctgccat agcgcatcag gatggcgttg
      721 accgggcagt tggtcacgct gatggcggtg ctggtgccgc cgtcaccggc ggcgatctcc
      781 tccagcacca gcgccaggct cacgtagtcg agatcggcgc cgccgtattc ctcgggcacg
      841 cagatgccgt aggcccccag tgccgccagg ccgcggtgcg cctcgtgggg gaaatagtgc
      901 tccttgtccc agcgcggcgc gttgggccag agctgttcgc gggcgaaagc gcgcaccgca
      961 tcgcggatca tttcctggtc ttgggtcagc agcatcgcgc atccaccttg ggttgggggc
     1021 tcaccatgtc ttgtaacggc ggccgtcgta gttgagaaag gcacccttgt gccggcgcgt
     1081 caggccggcg agggtcaggc gcatcgcggc gacactgcgc tccacgtcca gcggcgcacc
     1141 ggtgccgccg aggtcggttc gcacccaccc cggattgatc gccaccagcg tcgcccgcgg
     1201 atagtcgaac tgggccgcgg ccaccgccat gttgagggcc gccttgctca cgcggtaggt
     1261 ccagccatgg ctggacgcga cccccccgac gcggcccatc tcgctggtga cgaacgcaaa
     1321 gcgcccctcg gccgcctcca ccagcggcgc gatctgcacc agcgcctgca tggcgcccag
     1381 cacgttggtg tgcatgaccc ggtcgaactc ctgcggcagc ggctgcgcgg tggcgtcgcc
     1441 cctggcatag acgcccgcca cgtagatggc gatgtccagc ttctcgcctt cgagctgggc
     1501 gcgcagggcg ccggcgccgg ccgggtccgt cacgtccagc ctgtgagcgc gcgccccgag
     1561 cccctcgatg cgcccgagcc ccgcgtcatc gcgcgccgtg gcgaagacgc ggtcccattg
     1621 cccgcggtac tggcggacga actccaggcc gatgccgcgc gatgcgccga tgacgaggac
     1681 ggtggacaaa tgaacctcaa ccctgtcatt gcgggctggg ctcgcggaac gcccggggcg
     1741 cgcagctgtc cagtgcagcg agccccttgc cgggccagcg ctgtgcacgt accgaggcat
     1801 cacgctccgg cccggccccg caatccagga ttgccctcga gcatggatcc cggatcaagt
     1861 ccgggatgac aggccatagc gatgcccatc ggggcagccg cgctgccacc agtggaccga
     1921 tcagagcagt tccagcgcca ccgccgtgcc ttcgccgccg ccgatgcaca gcgtggcgat
     1981 gccgcgcttg ccgccgcggg ctttcagggc atggatcagc gtgaccatga tgcgagcgcc
     2041 ggaagcgccg atcgggtggc cgagggcgca ggcgccgccg ttcacgttca gcacctcgtg
     2101 gctgacgccg agctccttca tcagcgccat tggcaccacc gcgaaggcct cgttgacttc
     2161 ccacaggtcc acgtcgccgg ccttccagcc ggtcttggcc agcagcttct gcaccgcgcc
     2221 caccggcgcg gtggtgaacc attccggctc ctgcgagtgc atggcgtggc ccacgatgcg
     2281 ggcgatcggc ttgctgccgt aacgcacggc ggtggactcc ttcatcagca ccatcgccgc
     2341 ggcgccgtcg ttgatgctgg agctggacgc ggccgtgatg gtgccgtc
//
LOCUS       DASWIY010000500         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3665649, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000500 DASWIY010000000
VERSION     DASWIY010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3665649"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1808
                     /gene="istA"
                     /locus_tag="VGA59_17950"
     CDS             204..1808
                     /gene="istA"
                     /locus_tag="VGA59_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX9721605.1"
                     /translation="MSNVLSEEKRQQVIALGRLGWPLRRIQKETGIHRKTAGAYLKAA
                     GIAIRAPGGWGRRAAAKPANEVSPDWAASRGQNRPTVSPDSGPVPSPGRSPTGSACEP
                     YFDFIELSLSKGRNAKAIYQDLVDGHGFPGRYASVKRFVRQLRGQPSQPACAVIVTPP
                     GEEAQVDYGAGPMVRDPRTGRYRRTRLFVLTLGYSRKAVRLLTFQSSTRTWAELHEQA
                     FRRLGGATRTIVLDNLSEGVLKPDIYDPALNPLYRDMLAHYGAVALPCRVADPDRKGK
                     VERGVGHAKNTPLKGLRFESLEEAQTYLDHWEAKWADTRIHGTTKRQVAAMFAEEKPS
                     LLSLPLEPFRLYQFGERRVNLDGCVEVDAAYYSAPPGWIGRSVKVQWDGYVVRVLDPR
                     TGQLLREHRRQERGRHRIPDEDKPAQTPRPTTQLLARCGQIGPHVGAVAEQTYRRDGP
                     ESIRRIMGLTGLARKHGAALADDACAAALEIGLPANPYGFVRRWLERRPALTLRQVDP
                     IIRQLTLYRDLIDSKTSLPENQENSE"
     gene            1805..2545
                     /gene="istB"
                     /locus_tag="VGA59_17955"
     CDS             1805..2545
                     /gene="istB"
                     /locus_tag="VGA59_17955"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX9721606.1"
                     /translation="MNLTELQRSLRQLRLGGMAAVLQTRLQQAQAEPMAPIDLLATLV
                     SDELTRRSDRLLDRRRKLAAFRDPNKTLDNFDFTFNPKLNRSLVFDLATGAFIERRED
                     ALFLGPGGTGKSHLAQAIGQAAILQGHKVLYRETHILLEEVAEATIDGRRKQYMESIA
                     GVALLIIDDFGMRKLPQTAAEDLLEIVMRRYERASTLLTSNRPVDDWGKLLADVAAVG
                     AMLDRLLHHGHVLKCGPRSWRTKTAAAD"
     gene            2904..>3181
                     /gene="tnpA"
                     /locus_tag="VGA59_17960"
     CDS             2904..>3181
                     /gene="tnpA"
                     /locus_tag="VGA59_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX9721607.1"
                     /translation="MSELYQSLSHSKWDCKYHVVFVPKRRRKTIFGQVRRQLGQIFHG
                     LARQKECQILEGHLMPDHVHMCIAIPPKHPVASVIGFLKGKSAIAIARL"
BASE COUNT          631 a         1021 c         1002 g          527 t
ORIGIN      
        1 aagcacatct tgcacgaggt gctctcaaaa aagatctgaa gcccacgggg cgccgcaggc
       61 tggtgtgtgc gatacgccaa gggcaccagc tcagcgaaaa gcttcggggg acacccaaaa
      121 gcggccaatg agggacagct gaaaagcggc caatagagca ggggcaagac attgaggccg
      181 atgggggctt accctccgtc ggcgtgagca atgtcttgag cgaagagaag cggcaacaag
      241 ttatcgcgct ggggcggctg ggctggccgt tacggcgtat ccagaaagaa actggcattc
      301 accggaagac ggcgggagcc tatctgaagg ccgccggaat cgccatccgg gcgcccggcg
      361 gctgggggcg gcgcgcagcg gcaaaaccgg ccaatgaggt gtcccccgac tgggccgcct
      421 cacgcgggca aaatcgacca acggtgtccc ccgactctgg gccggtcccg agccccggcc
      481 gcagtcccac aggcagcgcc tgcgagccct acttcgactt catcgaactg tcgctcagca
      541 agggccggaa cgcgaaagcg atctaccagg acctggtcga cggccacggt ttcccagggc
      601 gctacgccag cgtcaagcgg ttcgtccggc agttacgcgg gcaaccttcg cagccggcat
      661 gcgcggtcat cgtcacgccg cccggcgagg aagcgcaggt cgactacggc gccggtccga
      721 tggtgcgcga tcctcgcacg ggccgctacc ggcgcacgcg cttgttcgta ctcacgttgg
      781 gctacagccg caaggccgtt cgcctgctca ccttccagtc cagcacgcgc acctgggccg
      841 aactgcatga gcaagcgttt cgccggcttg gcggggcaac gcgcacgatc gtgctcgaca
      901 acctcagcga aggcgtgctc aagcccgaca tctacgatcc ggcgttgaac ccgctgtatc
      961 gcgacatgct cgcgcactat ggcgccgtcg cgctgccgtg ccgcgtcgcc gatccggatc
     1021 gcaaggggaa agtggagcgc ggcgtggggc acgccaagaa caccccgttg aaaggactgc
     1081 gtttcgaaag cctagaggag gcccagactt acctggatca ctgggaagcc aagtgggccg
     1141 acacacgcat ccacggcacc actaagcgcc aggtagcagc catgttcgcc gaagagaagc
     1201 cgtcgctgct gtcactgccg ctggagccgt tccgcctcta ccagttcggc gagcggcgtg
     1261 tgaacctgga tggctgcgtc gaagtcgatg ccgcttacta cagcgccccg ccgggctgga
     1321 tcggccgcag cgtgaaagtg cagtgggatg gctacgtggt gcgtgtgttg gacccgcgca
     1381 ccggacaact gttgcgcgaa caccggcgcc aggagcgtgg ccgtcatcgc atccccgatg
     1441 aggacaagcc cgcgcagaca ccgcgtccca ccacgcaact gctggcgcgt tgcggccaga
     1501 tcggcccgca tgtgggtgcg gtcgccgaac agacctaccg ccgtgacgga ccagagtcca
     1561 tccgccgcat catgggattg accggcttgg cgcgcaaaca cggcgccgcc ctcgccgacg
     1621 atgcctgcgc cgccgcgctg gaaatcgggc ttcccgccaa cccctatggc ttcgtccgcc
     1681 gctggctcga gcgccggccc gccctcacgc tgcgccaagt ggatcccatc atccggcaac
     1741 tgaccttgta tcgcgacctc atcgacagca aaacttcgct gccagaaaac caggagaact
     1801 ccgaatgaat ctcaccgaac tacagcgcag cttgcgccaa ctccggctcg gcggcatggc
     1861 cgcggtgctg cagacgcggc tgcagcaagc acaagccgaa ccgatggcgc cgatcgacct
     1921 gctggccaca ctggtctccg acgagctgac ccgtcgctcg gaccggctct tggaccggcg
     1981 ccgcaaactc gccgcgttcc gcgatcccaa caagacgctc gacaactttg acttcacctt
     2041 caatccgaaa ctgaatcgca gtctggtctt tgatctggcc accggagcct tcatcgagcg
     2101 ccgtgaagat gcgctgtttc tcgggccggg tggcacgggc aaaagccatc tggcgcaggc
     2161 aataggccag gccgcgattc tgcaaggcca caaggtgctc tatcgcgaga cccatatcct
     2221 gctggaggaa gtcgccgagg cgaccatcga cggcaggcgc aagcagtaca tggaatcgat
     2281 cgccggcgtg gcgctgctga tcatcgatga cttcggaatg cgtaagttac cgcagacggc
     2341 cgccgaggat ctgctggaga ttgtgatgcg gcgttatgaa cgcgccagca cgttgttgac
     2401 ctccaacagg ccggtggatg actggggtaa actgctcgcc gatgtcgccg ccgtcggcgc
     2461 tatgctcgat cggctgctgc accatggaca cgtgctcaag tgcgggccca ggagctggcg
     2521 caccaaaacg gcggctgctg actgaagtac gaagaaggca tccgagcttt gatagatgcg
     2581 agggcgctgc cctcgcgctc ccgggatttt tcgcttttcc gccagaatgc cgcggaggcg
     2641 gctcagcgcc gcccacgcat tccgaccgct gggtcggcgc tctggtcgca tccctgcgga
     2701 gccatatcct ccgcccaggt gccgacagaa taaccgaaat tgcggtccgc cggcagagtt
     2761 tcattgccgc taagtgcggt agtgggttat aactgaatct ggtaagccac tggttcgacc
     2821 agtgagactg gaaaaggttt gaccgttgcg ggagtagaat ccccctcaag gtggcttgat
     2881 gatccacgat gaggaggatt ccaatgtctg agctttacca gagcctatcc cactccaaat
     2941 gggactgcaa gtaccacgtc gtgttcgtcc ctaagcgacg gcggaaaacc atctttgggc
     3001 aggtgcgtcg tcaactgggg cagatcttcc acggtctggc acgccagaag gaatgccaga
     3061 ttctggaagg gcacctgatg ccagaccatg tgcatatgtg catcgcgatt ccgcccaagc
     3121 acccggtggc ttcggtgatc gggtttctca aggggaagag cgcgattgcg attgcccggc
     3181 t
//
LOCUS       DASWIY010000501         7767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3679440, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000501 DASWIY010000000
VERSION     DASWIY010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3679440"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VGA59_17965"
     CDS             complement(<1..431)
                     /locus_tag="VGA59_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEX9721608.1"
                     /translation="MPPSLLNRRTAAVALVGWPVVWTGLRAQPTPARRPTPAQTEGPF
                     YPVSLPKDSDFDLLRNGALNYTRGQPAWVEGSVADLQGKPIAGAHVEIWQCDETGHYH
                     HPGDGGQADPSFQGFGRVTVGADGQYRFRTIRPAPYSGRTP"
     gene            complement(489..1160)
                     /locus_tag="VGA59_17970"
     CDS             complement(489..1160)
                     /locus_tag="VGA59_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX9721609.1"
                     /translation="MRELSDAGQGLDFVHADAHLLVVDKPAGLLSVPGRGAGKQDCLA
                     VRVQGLYADALVVHRLDMATSGLLLMARGAAMQRHLSRAFAQRQVGKRYVAVVAGRLA
                     AGPCESDGWALIDLPLAADWPNRPRRVVDLERGKPSQTRWRVLAYDAGADATRLELEP
                     ITGRSHQLRVHLQSLGHPILGDNLYAPPEVRARAPRLLLHASALRLVHPATDEPLAWS
                     SEPAF"
     gene            1159..1311
                     /locus_tag="VGA59_17975"
     CDS             1159..1311
                     /locus_tag="VGA59_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721610.1"
                     /translation="MSTMSSLPFSLRTVLVLMASNIFMKEPLKLDYFRVALCMVGAVY
                     FIFRGA"
     gene            1374..2021
                     /locus_tag="VGA59_17980"
     CDS             1374..2021
                     /locus_tag="VGA59_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEX9721611.1"
                     /translation="MLFGKLLPREGNFFEMFNQHADRIVEAARAYSSLVANYNDLNLR
                     EQYNREVDNAERAADRVTHEVNRLIHKTFITPIDRDQIHKLINTMDDVCDMIQDSAET
                     MALYDVRHMTDEIKRLTDLSVKCCERVKEAVYMIGKLADPATAEAALKTCEEIDKLES
                     DADRVMRSAMSKLFREEPDVREVIKLKAIYQLLESITDKCEDVANVIEGIVLEHS"
     gene            2038..3048
                     /locus_tag="VGA59_17985"
     CDS             2038..3048
                     /locus_tag="VGA59_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX9721612.1"
                     /translation="MENVQTALWVVILLVAMAIAFDFMNGFHDAANSIATVVSTGVLR
                     PGQAVVFAAFFNVVAILVFQLSVATTVGKGIVQPGVVDTHVVFGALLGAIAWNFVTWY
                     YGIPSSSSHALIGGIVGAVMAKSGAGSLIASGIWKTVLFIFVSPVLGFLLGSLMMVMV
                     SQLFRRATPSRVDRWFRRAQLVSAGAYSLGHGGNDAQKTIGIIWMLLIATGYVAAGAA
                     SPPIWVVIACYLAIGMGTMFGGWRIVKTMGQKITKLRPVGGFCAETGGALTLFLATAL
                     GIPVSTTHTITGAIVGVGSSRRASAVRWGVAGNIVWAWIFTIPASAFVSAIAYWLSLQ
                     IF"
     gene            complement(3246..3692)
                     /locus_tag="VGA59_17990"
     CDS             complement(3246..3692)
                     /locus_tag="VGA59_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721613.1"
                     /translation="MKNKTLATWLAFLGGPLGLHRFYLHGLGDLLGWVLPVPTALGLY
                     GIERIQRYGVDDQWSWVLVPLLGFTIAGCALTAIVYGLMTREKWNARFNRSLPEEAPA
                     GATNWFTIGAVVLSLLIGTSVLMASLAFSFQRYFEYQVEEGRKISQ"
     gene            complement(3689..4222)
                     /locus_tag="VGA59_17995"
     CDS             complement(3689..4222)
                     /locus_tag="VGA59_17995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9721614.1"
                     /translation="MSIIRPSNAEDLPAVTAIYAHHVLTGTGTFETTPPTPDEMAARR
                     ADVLAKDLPFLVAEDGGQILGFAYCQWFKPRPAYRFSAEDSVYLHPDAFGKGLGRRLL
                     AELAAQAEAGGIRKLIAVIGDSANAASIGVHRSLGFSTVGVFKSCGWKFGKWLDIVLM
                     EKALGQGDTTAPERAAG"
     gene            4343..4594
                     /gene="rpsP"
                     /locus_tag="VGA59_18000"
     CDS             4343..4594
                     /gene="rpsP"
                     /locus_tag="VGA59_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX9721615.1"
                     /translation="MVVIRLSRGGAKARPFFNIVVADKRTRRDGRFIERLGFYNPIAK
                     DNEESIRIAQDRLTYWRGVGAQASPTVERLIAQAAAKAA"
     gene            4677..5237
                     /gene="rimM"
                     /locus_tag="VGA59_18005"
     CDS             4677..5237
                     /gene="rimM"
                     /locus_tag="VGA59_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463704.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX9721616.1"
                     /translation="MFGLEPAELPADAIEVGRIADAWGVKGWFKVLPHSASPEALFSS
                     KRWYLQPSERGAKTFSGTVLLRVREAKEHSDTVVASAQDVDDRGAAEALKGARIFVAR
                     SSFPTAGNDEYYWVDLIGLEVVNREGVPLGRVRELLSTGPQTVLVIEYLEDGRVSERM
                     IPFVAVYIDDVDLPGKRITVDWQADY"
     gene            5263..6021
                     /gene="trmD"
                     /locus_tag="VGA59_18010"
     CDS             5263..6021
                     /gene="trmD"
                     /locus_tag="VGA59_18010"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX9721617.1"
                     /translation="MRFDVITLFPELFAPFLTGGVTRRAYESKQVDVRLWNPRDFAEG
                     NYRRVDDRPFGGGPGMVMMAAPLAQCLAAIRTQRADAVPVVLFSPVGDRLDHAGVERW
                     SAGSGAILVCGRYEGVDQRFIDRYVDVQISLGDFVLSGGEIAAMALLDAVARLQPGVL
                     ADQDSHQFDSFNPALDGLLDCPHYTRPEDWEGLKAPAELLSGHHVHIERWRRDRRLEM
                     TGRRRPDLIAAARAAGRLSADDEAVLAKAGITCL"
     gene            6124..6501
                     /gene="rplS"
                     /locus_tag="VGA59_18015"
     CDS             6124..6501
                     /gene="rplS"
                     /locus_tag="VGA59_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX9721618.1"
                     /translation="MNLIQTLEQEEIVRLGKKIPAFAPGDTVIVSVNVVEGSRKRVQA
                     YEGVVIAKRNRGLNSGFTVRKISSGEGVERTFQTYSPLIASIEVKRRGDVRRAKLYYL
                     RERSGKSARIKEKLAHRNKPAAA"
     gene            6563..7270
                     /locus_tag="VGA59_18020"
     CDS             6563..7270
                     /locus_tag="VGA59_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX9721619.1"
                     /translation="MPINPDPADTPLSKLPNFDPRTVPVLGIDSHLPAVGREALAPAA
                     LRERFLAPPQWEPEIWVERKFSDRPLSRASVLVPVVMRERPTVLLTERTTHLSTHSGQ
                     VAFPGGRRDETDTDAAHTALREAHEEIGLDAGLIDVIGQMPTYTTGSQFLITPVVALV
                     SPDHRLTLNTFEVADAFEVPLEFLMNPAHHRRHAIEWAGARREWFSMPYMDGTSERFI
                     WGATAAMLRNFYRFLSA"
BASE COUNT         1305 a         2569 c         2604 g         1289 t
ORIGIN      
        1 tgtggcgtgc ggccgctgta cggcgccggg cgaatggtgc ggaagcggta ttggccgtcc
       61 gcgcccacgg tgacacggcc gaacccctgg aatgacggat cggcttggcc accgtccccc
      121 ggatggtgat agtggcccgt ctcgtcgcat tgccagatct cgacgtgggc gccggcgatg
      181 ggcttgccct gcaggtcggc cacgctgccc tcgacccatg cgggctggcc ccgcgtgtag
      241 ttcagggcgc cgttgcgcag caagtcgaaa tcggaatcct tgggcaggct cactgggtag
      301 aacggtccct cggtttgtgc cggcgtcggg cgtcgcgcag gcgtcggctg ggctcgaagc
      361 ccggtccaga cgacgggcca gccgaccagg gcgactgcgg cggtgcggcg gttgagcagg
      421 gaagggggca tggcggtctc cacggcgtcc tggtctgagg cgcgcgggcc ggtccgagtt
      481 ccgcgggtct agaaggcagg ctcgctggac catgcgagcg gctcgtccgt tgcgggatgc
      541 accagccgca aggcgctggc atgcagcagc aggcgcggcg cgcgcgcccg cacttcgggc
      601 ggggcgtaca ggttgtcgcc caggatcggg tggcccaacg attggaggtg cacgcgcaac
      661 tggtgggatc tcccggtgat gggttcgagt tccagccggg tggcatcggc gccggcgtcg
      721 taagccagca cccgccagcg cgtctggctc ggcttgccgc gctccaggtc gacgacgcgg
      781 cgcgggcgat tcggccaatc ggcggccagg ggcaggtcga tcagcgccca gccatccgat
      841 tcgcacggcc cggcagccag gcggcccgcg acgaccgcga catagcgctt gccgacctgc
      901 cgctgcgcga aggcacggct caggtgccgc tgcatggccg cgccgcgggc catcagcagg
      961 aggccggagg tggccatgtc gagccggtgg accaccaggg cgtccgcgta caggccctgc
     1021 actcgcacgg ccaggcagtc ctgttttccg gcgccacgcc ccggcacgct gagcaggccg
     1081 gccggcttgt caacgaccag caagtgcgca tcggcatgga cgaagtccaa cccctggcct
     1141 gcatcggata attcacgcat gagcacgatg tcctcgcttc ctttctcgtt gcggacggtt
     1201 ctggtgctga tggcctccaa tatattcatg aaggagccgc tcaagctcga ttatttccgg
     1261 gttgcgctgt gcatggtcgg ggcggtgtat ttcattttcc gcggcgccta aaggcgcggc
     1321 gacgccaagt tacactcgcc acaaacccga acaaacccaa ccaggagccc gcaatgctgt
     1381 ttggcaagct gctgccgcgc gaaggcaatt tcttcgagat gttcaaccag catgcggacc
     1441 gcatcgtgga ggcggcgcgg gcctactcga gcctggtggc gaattacaac gacctgaacc
     1501 tgcgcgagca gtacaaccgc gaagtggaca acgccgagcg ggcggccgac cgggtcacgc
     1561 acgaggtcaa tcgcctgatc cacaagacct tcatcacgcc gatcgatcgc gaccagatcc
     1621 acaagttgat caacacgatg gacgacgtct gcgacatgat ccaggattcg gcagagacca
     1681 tggcgctgta tgacgtgcgc cacatgaccg atgagatcaa gcgcctgacg gacctgagcg
     1741 tcaagtgctg cgagcgggtc aaggaagcgg tctacatgat cggcaagctg gccgacccgg
     1801 ccacggccga ggccgccctg aaaacctgcg aggaaatcga caagctcgag tcggacgcgg
     1861 accgcgtcat gcgctcggcc atgagcaagc tctttcgcga agagcccgac gtgcgcgaag
     1921 tgatcaagct caaggccatc taccagctgc tggaaagcat caccgacaag tgcgaggacg
     1981 tcgccaacgt catcgagggc atcgtcctcg agcattcctg aaagccgggg cccggcgatg
     2041 gaaaacgtgc aaaccgccct gtgggtggtg atcctgctgg tcgcgatggc gatcgctttc
     2101 gacttcatga acggtttcca tgatgcggcc aactcgatcg ccaccgtggt ttccaccggc
     2161 gtgctcaggc cgggccaggc cgtcgtgttc gcggcgttct tcaacgtggt ggcgatcctg
     2221 gttttccagc tgagcgtcgc aaccacggtg ggcaagggga ttgtgcagcc gggcgtggtc
     2281 gacacgcatg tggtgttcgg ggcgctgctc ggcgccatcg catggaattt cgtgacctgg
     2341 tattacggga tcccgagcag ttcttcccac gcgctgatcg gcggcatcgt cggggccgtg
     2401 atggccaagt ccggtgcggg gtcgctcatc gcatcgggca tctggaagac ggtgctgttc
     2461 atcttcgttt ccccggtgct gggtttcctg ctcggctcgc tgatgatggt catggtgtcc
     2521 cagctgttcc ggcgcgccac gccttcgcgg gtggaccgct ggttccggcg cgcgcagctc
     2581 gtttcggccg gcgcgtacag cctgggccac ggcggcaacg acgcgcaaaa aacaatcggc
     2641 atcatctgga tgctgctgat cgccacgggc tacgtggcag ccggtgcggc gtccccgccg
     2701 atctgggtgg tgatcgcttg ctacctggcg atcggaatgg gcacgatgtt cggcggctgg
     2761 cgcatcgtca agaccatggg ccagaagatc accaagctgc ggccggtcgg cggcttctgc
     2821 gcggaaaccg gtggcgcgct gaccttgttc ctggccacgg cgctcgggat tcccgtgtcg
     2881 accacccaca ccatcacggg cgccatcgtc ggtgtcgggt cgtcccggcg ggccagcgcg
     2941 gtgcgctggg gcgtggcggg caacatcgtc tgggcctgga tcttcaccat ccccgccagc
     3001 gcgtttgtct ccgcgatcgc ctattggctg agcctgcaga ttttctgagg ggttgtcatt
     3061 gcgggctggg ctcgcggaac gcccggggcg cgcagctgtc cagtgcagcg agcccaatgc
     3121 cgggcctgcg ctatgcacgt accgaggcat cacgctccgg cccggcgccg caatccatga
     3181 attctggagt catggatccc ggatcgagtc cgggatgaca gtccgtgccg ggatgacggc
     3241 cgcttctatt gcgaaatctt gcggccttcc tccacctggt actcgaaata gcgctggaag
     3301 ctgaaggcga ggctggccat cagcacggaa gtgcctatga gcagcgacag caccaccgcg
     3361 ccgatggtga accagttggt cgcgccggcg ggggcctctt cgggcagcga acggttgaac
     3421 cgggcattcc acttttcgcg cgtcatcagg ccgtaaacga tggccgtgag ggcgcagccg
     3481 gcgatggtga accccagcag cggcaccagc acccagctcc attggtcatc caccccataa
     3541 cgctggatgc gctcgatgcc gtacaggccc agcgcggtgg gaaccggcag gacccagccg
     3601 aggaggtcgc ccaggccgtg caggtagaag cggtgcaggc ccaggggccc gcccaggaat
     3661 gccagccagg tggcgagcgt cttgttcttc atcctgcggc gcgctcgggt gcggtggtat
     3721 cgccttggcc caaggccttt tccatcagca cgatgtccag ccacttgccg aacttccagc
     3781 cgcaggactt gaaaacgccc acggttgaaa agccgagcga ccggtgcacg ccgatcgagg
     3841 cggcattggc cgagtccccg atgacggcga tgagcttgcg gatgccgccg gcctcggcct
     3901 gcgccgccag ttcggccagc aacctgcggc ccaggccctt gccgaacgcg tcagggtgca
     3961 ggtagaccga gtcctcggcc gagaagcggt aggccggccg cggcttgaac cactggcagt
     4021 aggcaaagcc caggatctgc ccgccgtcct cggccaccag gaagggcagg tctttggcca
     4081 gcacgtcggc gcggcgcgcc gccatctcgt ccggggtggg cggcgtggtc tcgaaagtgc
     4141 ccgtgccggt gagcacgtga tgggcgtaaa tcgcggtcac ggcgggcaag tcctcggcgt
     4201 tgctggggcg gatgatgctc ataaaagtca gaaacgttat aatcgcaggc tctacagcgt
     4261 gtcgccggcc gggtggctcg cgcgtgtctc aacgctggaa agacttcagg ggaaattttt
     4321 cccctccacc caaggataaa tcatggtcgt cattcgactc tcccgcggcg gcgcgaaagc
     4381 ccgccccttc ttcaatatcg tcgttgccga caagcgtacc cgccgcgacg gccgcttcat
     4441 cgagcgcctc ggtttctaca accccatcgc caaggacaac gaggaaagca tccgcatcgc
     4501 ccaggaccgc ctgacgtact ggcgcggcgt gggcgcccag gcctccccga ccgtggaacg
     4561 cctgatcgcg caggccgccg ccaaggcagc ctaagcaagt ggcccccacg ctcggcgtcg
     4621 cctcgctgcc ccccgagggg gccgcagtcg ccttggggcg gcccggcggc gcctgaatgt
     4681 tcggcctcga acccgccgaa cttccggcgg acgccatcga agtcggtcgc atcgccgatg
     4741 cctggggcgt caagggctgg ttcaaggtcc tgccccacag cgcttcgccc gaagcgcttt
     4801 tttcttccaa gcgctggtac ctgcaaccgt ccgaaagagg cgccaagacc ttttccggca
     4861 cggtgctgct gcgtgtgcgc gaggccaagg agcattcgga caccgtcgtg gccagcgccc
     4921 aggacgtgga cgaccgcggc gcggccgaag cgctcaaggg cgcccgcatt ttcgtcgcgc
     4981 gctcgagctt ccccaccgcc ggtaacgacg agtactactg ggtcgacctg atcggcctgg
     5041 aggtcgtcaa ccgcgagggg gtgccgcttg gccgggtgcg cgagctgctc tcgaccggcc
     5101 cccagaccgt gctggtgatc gaatacctgg aagacggcag ggtgtcggag cggatgatcc
     5161 cgttcgtcgc cgtctatatc gacgacgtgg acctgccggg caagcgcatc acggtcgact
     5221 ggcaagccga ttattaggcc cgtcacggcc taataatcca tcatgcgctt cgacgtcatc
     5281 acgctctttc ccgagctgtt cgctcccttc ctgaccggcg gcgtcacgcg gcgcgcctac
     5341 gagtcgaaac aggtcgacgt gcgcctttgg aacccgcgcg acttcgccga gggcaactac
     5401 cgccgcgtcg acgaccgtcc gttcggcggc gggccgggca tggtcatgat ggccgcaccg
     5461 ctggcccaat gcctggcggc gatcaggacc cagcgcgccg atgccgtgcc ggtggtgctg
     5521 ttttcgcctg tcggcgacag gctggaccac gccggggtcg aacgctggtc ggccggcagc
     5581 ggcgccatcc tcgtttgcgg gcgctacgaa ggggtggacc agcggttcat cgaccggtat
     5641 gtcgatgtcc agatcagcct cggcgatttc gtgctttcgg gcggtgagat cgcggccatg
     5701 gctttgctgg acgccgtggc gcgcctgcag ccgggtgtcc tggccgacca ggacagccac
     5761 cagttcgaca gtttcaatcc cgcgctcgac gggctactgg actgccctca ctacacccgg
     5821 cccgaggact gggagggcct gaaggcgccg gccgaattgc tctcgggcca tcatgtccac
     5881 atcgagcgct ggcggcgcga ccggcgcctc gaaatgacgg gccggcgccg ccccgatctg
     5941 atcgcggccg cccgtgccgc cgggcggctg tccgccgacg acgaagccgt cttggccaag
     6001 gccggcataa cttgtttata atcaaaggct tttcgatcct ctacccggcc gcccgacatg
     6061 gcatgcgaca agcgcatccc aggggccttt tgcgcagcgc gggcacgatc accgaggtaa
     6121 cccatgaatc tgatccagac cctagagcag gaagagattg tccgtttggg caagaaaatc
     6181 cctgcattcg cgcccggcga caccgtgatc gtcagcgtca acgtcgtcga aggcagccgc
     6241 aagcgcgtgc aggcgtatga aggcgtcgtg atcgccaagc gcaatcgcgg cctgaacagc
     6301 ggcttcaccg ttcgcaagat ctccagtggc gaaggcgtgg agcgcacgtt ccagacctac
     6361 agcccgctga tcgccagcat cgaagtgaag cgccgcggcg acgtgcgccg cgccaaactg
     6421 tattacctgc gcgagcgcag cggcaagtcg gcccgcatca aggaaaagct ggcccaccgc
     6481 aacaagcccg ccgccgcata ggcccaggct ctccccgaac cgccgccggc catcacgccg
     6541 gcggcggttt ttccattcct agatgcccat caatccggac cctgccgaca cgccgctgtc
     6601 caagctgccg aatttcgatc cccgcaccgt gcccgtgctc gggatcgact cgcacttgcc
     6661 cgcggtggga cgggaggcgc tagccccggc cgcgctgcgc gagcgtttcc tggcgccgcc
     6721 gcagtgggag ccggaaatct gggtcgagcg caagttctcc gatcggccgc tttccagggc
     6781 ttcggtactc gtcccggtcg tgatgcgcga gcggcccacg gttctcttga ccgagcgcac
     6841 cacccacctg tccacccact ccggccaggt ggcgtttccc ggcggcaggc gggatgagac
     6901 cgacaccgac gccgcccaca cggccctgcg cgaagcgcat gaggaaatcg ggctggacgc
     6961 cggcctgatc gacgtgatcg ggcagatgcc gacctacacc acgggcagcc agttcctcat
     7021 cacgccggtg gtggcgctgg tcagtcccga ccatcggctc acgctcaaca ccttcgaggt
     7081 ggccgacgcc ttcgaggtgc cgctggaatt cctgatgaat ccggcccatc accgccgcca
     7141 cgcgatcgag tgggcgggtg cgcggcgcga atggttctcc atgccctaca tggacggcac
     7201 ctccgagcgt ttcatctggg gcgccacggc cgccatgctg cgcaacttct accgcttcct
     7261 cagcgcgtag gatgtgaccc ccacgcttgc cgcctggcgg ctgcgctgcc cccgaggggg
     7321 cccagtcgcc ttgtgagcgg cccggcggcg actgacctcg cggctgatat cgcccgtatc
     7381 attgctgcat ggtgcgtcgt gacgcgaaga acgaactgac tccgaaaggg gaagggacgg
     7441 actttgcttg ctacgcctgt ccagcgggtg agagtcccgt cccggtaagc gtcggtgcgc
     7501 cgggtagcag gccccaggtg gcacggggag acccggctgc ccgagcgggg cgtcgaaagc
     7561 ccgagggaca gcttgagctg ttcggagggg agccagcacg cgggccgcaa catgaagtga
     7621 agcctgcagc ctcgacagat gtacaacccg caggccgagc cgatcatgtc acggcgaagg
     7681 caacatcggg cgcgccagaa ccgaacggtg cgcaagactg cggcggggta tggggagcgg
     7741 cacgtgtgca aggacaggtg tggaaca
//
LOCUS       DASWIY010000502         3706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3687149, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000502 DASWIY010000000
VERSION     DASWIY010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3687149"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VGA59_18025"
     CDS             complement(<1..822)
                     /locus_tag="VGA59_18025"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX9721620.1"
                     /translation="MLFALFTLSGFTGLIYESIWSHYLKLFLGAAAFSQSFVLAAFMG
                     GLALGAWIASRATKRIVNLLAAYGWIEAGIGLAALVFHEVFVWLTQASLDHVIPALGS
                     PLTVEIYKYTLCGLLIVPQTVLLGMTFPLMSGAVIRRSPEASGHHLAMLYFTNSIGAA
                     AGALAAAFLLLGWIGMPGTMRAAGVLNLALAAAVLALARAGEPQPPAAVPAAGAAPAS
                     AYLVRLFLAALQDMQHPRHPVEGLVVDENGGERRQPHGKLHHAREAHRIGDAVDAA"
     gene            957..1640
                     /locus_tag="VGA59_18030"
     CDS             957..1640
                     /locus_tag="VGA59_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9721621.1"
                     /translation="MINVFIGYDSRETVAYSVLAYSIQARASQPVSVTPLMLSQLKEV
                     LVRERHPLQSTDFSFSRFLTPYLSGYTGWSVFMDCDMLMREDVADLWKLREDRYAVMV
                     VKHHHVPRETVKFLGEPQSKYEKKNWSSVMLFNNARCRALSPEYVNRASGLELHQFKW
                     LGNDDLIGALPGRWNHLVGYNPPRRDAGLVHYTLGGPWFDDYRDCEYSREWFTERDTM
                     LRTASRAAP"
     gene            1694..3166
                     /locus_tag="VGA59_18035"
     CDS             1694..3166
                     /locus_tag="VGA59_18035"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX9721622.1"
                     /translation="MSNREARERAGAIAPPSVAAETPLFVDLDGTLIHGDVLLEASFG
                     LLRRNLLYLLLLPIWLARGKAYLKRQIAHRVELDAANLPFNEPFLEFLRAEHARGRPL
                     VLATATHRRYAEQVSRLLGIFAGVLATEDALNLSGEVKLQAILAYSRGRDFEYAANAR
                     RDVAIWRHARGAVLVNADHRTRTAAARVASVTAVFERPAAGWRAYARALRVPHWAKNL
                     LLFVPLLTAHQWGNPDALQQAVLAFAAFCLCASGSYLLNDLIDAPFDRQHPRKRMRPL
                     AAGVISPLSALVLPPILVFAALVIAAQLPRGFLWVLVGYAGLAAGYSLYLKHRALVDV
                     LILAGLHTLRVIAGAIAIEVALSFWLLAFSVFLFFSLAVAKRCAELRAQGGVPEAALA
                     GRDYRVSDLPVLRAMGLASGYVAVLVFALYIDSADIAARYSHPQWLWLSCPALLYWIS
                     HVWIKEGRGEMHDDPLMFALRNPASYAVLTVLLATVIRAL"
     gene            3256..>3706
                     /locus_tag="VGA59_18040"
     CDS             3256..>3706
                     /locus_tag="VGA59_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721623.1"
                     /translation="MHIAARPGFADRALSATALAAAIVVVVLLTFVPEGSNDYWLQVK
                     VGGMIWNDGELPRSVLFTFTEARDFPFHAHEWLPSVVFYLLDVSLGHERLIWVNGALG
                     VAIFGLCYRLAHRLTSSLPLSLLLALVTMTAVNYRHFLRPEIFACLFV"
BASE COUNT          600 a         1186 c         1236 g          684 t
ORIGIN      
        1 cgccgcatcg accgcatcgc cgatccggtg cgcttcgcgg gcatggtgca gcttgccatg
       61 gggctggcgg cgctcgccac cattttcgtc taccaccaga ccttcgactg gatggcgtgg
      121 gtgctgcatg tcctgcagcg cggcgaggaa caaccgcacc aggtaggcgc tggcgggtgc
      181 cgcgccggcg gcgggcaccg ccgcgggcgg ctgcggctcg cccgcccgcg cgagcgcgag
      241 caccgcggcg gccagtgcaa ggttcaacac gccggccgcg cgcatggtgc ccggcatgcc
      301 gatccagccg agcagcagga acgccgccgc cagcgccccg gccgcggcgc cgatcgaatt
      361 ggtgaaatag agcatcgcca ggtggtggcc gctcgcttcg gggctgcgcc ggatcaccgc
      421 gccgctcatc aacgggaagg tcatgccgag cagcaccgtc tgcgggacga tgagcagacc
      481 gcacagcgtg tacttgtaga tttcaacggt gagcggtgag ccgagcgccg ggatgacgtg
      541 atcgagcgac gcctgcgtga gccacacgaa cacctcgtgg aataccaggg ctgcaaggcc
      601 gatccccgct tcgatccagc cgtaggcggc gagcaggttc acgatccgct tggttgcgcg
      661 gctcgcgatc caggcgccaa gcgccaagcc gcccatgaac gcggccagca cgaaggactg
      721 ggagaacgcg gcggcgccga ggaagagctt caggtaatgc gaccagatgg attcgtagat
      781 gagccccgtg aaacccgaca aagtgaaaag ggcgaacagc aactggacgt tccgattggg
      841 catgttccgt cctatcgtcg aaagaccgca gtctaacgta gcgcggcaag accggccatg
      901 taattctgcg gggtaatggc cgtgtgatat ccttcacaaa aagagattcg cacgacatga
      961 tcaacgtctt catcgggtac gactcgcgcg agacggtcgc ttacagcgtc ctcgcataca
     1021 gcatccaggc gcgcgcttcc cagccggtct ctgtcacacc gctcatgctg tctcaattga
     1081 aggaggtgct cgtccgcgag cgccatccgc tccagagcac ggatttctcg ttctcgcgct
     1141 tcctgacgcc gtacctttcc ggttacaccg ggtggtccgt gttcatggac tgcgacatgc
     1201 tgatgcgcga ggacgtcgcc gacctctgga aactgcggga ggatcgctac gcggtgatgg
     1261 tggtcaagca ccaccacgtc cccagggaaa cggtaaaatt cctgggcgag ccgcagagca
     1321 agtacgagaa gaagaactgg tcgagcgtga tgctgttcaa caacgcccgg tgccgggcgc
     1381 tgagccccga atacgtgaat cgtgcttcgg gcctcgagct gcaccagttc aagtggctgg
     1441 ggaacgacga tctcatcggg gcgctgcccg ggcgctggaa ccatctcgtg ggctacaatc
     1501 cgccgcgccg ggatgccggc ctcgtccact acacgctcgg cggcccctgg ttcgatgact
     1561 atcgggattg cgagtattcg agggagtggt tcactgagcg ggacacgatg ctacgcaccg
     1621 cgagccgcgc ggcgccgtag atggggcagg agccaggacc gggctccccg agcggggaga
     1681 gaaggggtat cgcgtgagca accgtgaagc ccgcgagcgc gcgggggcca tagcgccgcc
     1741 atccgtagca gccgaaacgc cgctgttcgt tgatctcgat ggcacgctca tccacggaga
     1801 cgtgctgctg gaagccagct tcggactgct tcggcgcaat ctcctctatc ttcttctgct
     1861 gccgatctgg ctcgcccgcg gcaaggccta tctgaagcgg caaatcgcgc accgcgtcga
     1921 gctcgatgcg gcgaatctgc ccttcaacga gccgtttctc gaattcctga gggccgagca
     1981 cgcccgcggg cggccgctcg tccttgcaac cgcgacgcac cgtcgctacg cggagcaggt
     2041 ctcgcggctc ctggggatct tcgcgggcgt gctggctacc gaagacgcgc tcaatctttc
     2101 cggcgaggtg aagctgcagg caatcctcgc ttacagcagg gggagggatt tcgagtatgc
     2161 cgcaaatgcg cgccgggatg tcgctatctg gcgccacgcc cgtggcgctg ttctcgtcaa
     2221 cgcggaccac cgcacgcgga ccgctgcggc gcgcgtcgca agcgtgacgg ctgtattcga
     2281 gaggcccgcg gccggatggc gcgcgtatgc acgggcgctg cgcgtgcctc attgggcgaa
     2341 gaatctgctg ctgttcgtgc cgctccttac cgcgcatcag tggggcaatc ctgacgcgct
     2401 ccagcaggct gtgctggctt tcgcggcgtt ctgcctgtgc gcatcgggaa gttatctgct
     2461 gaacgacctt atcgacgccc ctttcgaccg gcagcatccg cgcaagcgca tgcgtccgct
     2521 cgctgctgga gtgatttccc ccttgtccgc gctggttctg ccgccgatcc tggtgttcgc
     2581 cgcgctggtg atcgcggcac agctgccgcg aggatttctc tgggtgctgg tcggctatgc
     2641 cgggctggcg gcgggctatt cgctctatct caagcaccgc gcgctggtcg acgtgctgat
     2701 attggcgggg ctgcacacgt tgcgcgtgat cgccggggcg atcgccatcg aggtcgcgtt
     2761 gtccttctgg ctgctcgcct tctcggtatt cctgttcttc agcctggcgg tggccaagcg
     2821 ctgtgcggag ttgcgtgcgc agggcggagt cccggaggcg gcgctcgccg ggcgcgacta
     2881 tcgcgtgagc gacctgccgg tgctgcgcgc catggggctg gcgagcgggt acgttgccgt
     2941 gctggtgttc gccctgtaca tcgacagcgc ggacatcgcg gcccgctaca gccacccgca
     3001 atggttgtgg ctgtcatgcc cggcattgct ctactggata agccatgtct ggatcaagga
     3061 aggtcgtggc gaaatgcacg atgacccgct catgttcgcc ctgcgcaacc ccgcgagtta
     3121 cgccgtattg acggtgctgc tggcgacggt aattcgggcc ctctagccgc atggactgcg
     3181 tctgccccgt cacgtgcgtg gcgccgaagg agcgcgtgtt ccgcgcgcag ccgccaatgc
     3241 ggacaacctg acccgatgca tatcgcggcc cgtcccggat tcgccgatcg ggcgctcagc
     3301 gcgaccgcgc tggccgcagc catcgtcgtc gtggtcctgc tgacgttcgt gccggaagga
     3361 agcaacgact actggctgca ggtgaaggtc ggcgggatga tctggaacga cggggagttg
     3421 ccgcggagcg ttctgttcac gttcacggaa gcacgtgatt tcccgtttca cgcccacgag
     3481 tggctgccat cggtcgtgtt ctacttgctc gacgtgtctc tgggccatga acgcctcatc
     3541 tgggtgaacg gagcgctggg cgtcgcgatc ttcggtctgt gctaccggct cgcgcaccgt
     3601 cttacgtcga gcctcccctt gtcgctactt ctggcgctgg tgacgatgac ggccgtgaat
     3661 tatcgccact tcctgcggcc ggagatcttc gcgtgcctgt tcgtgc
//
LOCUS       DASWIY010000503         7801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3687518, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000503 DASWIY010000000
VERSION     DASWIY010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3687518"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VGA59_18045"
     CDS             complement(<1..481)
                     /locus_tag="VGA59_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX9721624.1"
                     /translation="MDMQGSRALAVTQQQAWDALNNPQVLRACIPGCDKFEPTGENQY
                     AIGVAVKIGPLAARFAGKIGLSDIKPPDSYTISFDGQGGAAGFGKGNSGVTLTPQGTG
                     CELAYTVHAQVGGKIGLSNIKPPDSYTISFDGQGGAAGFGKGNSGVTLTPQGTGCELA
                     "
     gene            complement(666..1001)
                     /locus_tag="VGA59_18050"
     CDS             complement(666..1001)
                     /locus_tag="VGA59_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9721625.1"
                     /translation="MTSPFSSARCARTFPWSPVTFTFDGSLAFCAALEYHCFMQLATG
                     TVIDGKVVIEGLALPEGTVVTVLTRDDEQAVRLSPAEEAQLLEALDEADREEGISAEE
                     LFARLQRFG"
     gene            complement(1030..2223)
                     /locus_tag="VGA59_18055"
     CDS             complement(1030..2223)
                     /locus_tag="VGA59_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9721626.1"
                     /translation="MQLGDARTGKLADNITGFGRALRRAGVRTDSARIALAQQAAQLV
                     GVSSKDDLGAAMEAVMVSREQDRLVFRELFDAYFRDPEVANKLLAQLLPSAEGRAEPS
                     KRRPRVREALSPQQAFGPSRPTKQDQKVDFDAAMTASDLVRLKHADFNALSAGEYGLI
                     ERLARDIALPIPCVPSRRLHPGERGLRVHWPGVMRRAAGTGGELVQLQRLQRHQQPLP
                     LLVLVDVSGSMERYARLLLAFLHAATRGGGGRVGRRDVFAFGTHLTDLTPAFRLADTD
                     AMLAAAGMAISDFAGGTRLGESLATLRQQHARRLVGRRTLVLAITDGLDTGAPDELER
                     ELDWLRLRSRRLLWLNPLLRFDGYAPTARGAAVLHRQAHGMLAVHNISKLEELAASLA
                     ALMKR"
     gene            complement(2376..3281)
                     /locus_tag="VGA59_18060"
     CDS             complement(2376..3281)
                     /locus_tag="VGA59_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9721627.1"
                     /translation="MFASIDALTQGLQGVGYFADRRLATAVFLALRLQRPLLLEGEPG
                     VGKTELAKALALALPRDLLRLQCYDGLEQREALYEWNYAAQLLHMRAAEMRHGGPADT
                     AEVEREVYQDRYLIRRPLLQALQAPAPGAVLLIDEVDRADEPFEAFLLEYLGEYQVSI
                     PEIGTVRAVATPVTILTSNRTRELNDAVKRRCLYHWLDYPDRDRELEIVRAQVPGASE
                     ALARQVAGFVGKLRSQPFADAFQRAPGIAESVEWAKALVALDTLVVDPEVVADTAGIL
                     FKQREDVAALTRDLATELLKPEEVA"
     gene            complement(3282..4067)
                     /locus_tag="VGA59_18065"
     CDS             complement(3282..4067)
                     /locus_tag="VGA59_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX9721628.1"
                     /translation="MYAFTFERPTTAADAARLAASGATPLAGGQTLLASMKLRLSSPG
                     TLADLGGIKELAGIRKDGNNLVIGAMARHAEVAESDVVKTAIPALADLAGHIGDKQVR
                     ALGTLGGSVANNDPAACFPSAVLGLGATVQTTKRQIAADDFFLGLFTTALAEDELITA
                     ISFPVPKRAAYMKFKQPASRFALIGVFVAQTDGGVRVAVTGGGNGVFRHKGLEDALNK
                     SFTPAAAAAVKIDAGDLSSDIHASAAYRANLIGVMTQRAVAKV"
     gene            complement(4080..6452)
                     /locus_tag="VGA59_18070"
     CDS             complement(4080..6452)
                     /locus_tag="VGA59_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9721629.1"
                     /translation="MGASDFSKLPHIGEALRRKEDYRFLTGAGQYTDDVNLGHQNYAV
                     FVRSPHAHAVIKGIDVAKAQALPGVVKVFTGKDVEGKMGGLPCGWLITSTDGQPMKEP
                     PHPILAQGKVRYVGDHVAMVVADTIEHAKNAAEAVEVDYEVLAAVVRVQDAKNGPALH
                     EAAPDNHCYKWAIGDKAAVDAVFASAAHVTRLDLVNNRLIPNAIEPRAAIGSYNRATD
                     EYTLYVANQNPHVERLLMTAFVLSLPEHKVRVIAPDVGGGFGSKIFLYAEDVALTWAA
                     KQLNCAIKWTAERSESFLCDAHGRDHVSHAEMAMDKDGKFLALRVHTDANLGAYLSTF
                     STAVPTILYATLLAGEYTTPQIHVEVDAWFTNTAPVDAYRGAGRPEATYLLERLVSRC
                     AWELGLGQDEIRKRNFITSFPYQTPVALQYDVGDYHACMDKAKALAEVAGFDARKAAS
                     AANGLKRGIGYSSYIEACGIAPSNIAGALGARAGLFECGEIRVHPTGSVTVFTGSHSH
                     GQGHETTFAQVVAARLGITVENVDVVHGDTGRVPFGMGTYGSRSISVGGAAIMRALDK
                     IETKAKKIAAHLMEASDADVEFANGEFTVKGTDKKVTFGQVALTAYVPHNYPLDKLEP
                     GLNETAFYDPTNFTFPAGTYICEVEVDTATGTTRIDRFTAVDDFGTIINPMIVEGQVH
                     GGLVQGIGQALLENCVYDPESGQLLTGSFMDYAMPRADDFPMFKVDTVCTPCSHNPLG
                     TKGCGEAGAIGSPPAVINAVLDALRDLGVKDLDMPASPSRVWEAIQAARP"
     gene            complement(6466..6936)
                     /locus_tag="VGA59_18075"
     CDS             complement(6466..6936)
                     /locus_tag="VGA59_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9721630.1"
                     /translation="MQVQLTVNGKSASVDIAPNTLLVHVLREHLQLTGTHVGCDTAQC
                     GACTVIVNGRAIKSCNTLAVQHEGAEVTTIEGLAAADGTMHPMQAAFKECHGLQCGFC
                     TPGMVMSAVDLCQHNPGAGEAEIRELLEGNLCRCTGYQNIVKAVQLGQTAMASA"
     gene            7138..>7801
                     /locus_tag="VGA59_18080"
     CDS             7138..>7801
                     /locus_tag="VGA59_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9721631.1"
                     /translation="MTVPIQTIAEQRLRQIELARRAVMVEGRSATDALAHAWFEGHWI
                     ERSWRRCLASGQRPEQRLGFDMIAAQARQRVIDASQPLVRAARPVLDRLSRALASTRY
                     FAILTDHDGIVIDAHGPIDRSDRRADLITRIGVDLSERSVGTSAIGAALAELQPVWLH
                     RGEHFFADTSVYSCAGAPLFGPDGRCVGMLDLTGINAAERPELKHLVAQSARSIENAL
                     TLA"
BASE COUNT         1232 a         2628 c         2578 g         1363 t
ORIGIN      
        1 aggccagttc gcagcccgtg ccctgcggcg tcagcgtcac gccggaattt cccttgccga
       61 atccggccgc gccgccctgg ccgtcgaagc tgatggtgta actgtcggga ggcttgatgt
      121 tcgaaagacc gatcttgccg cccacctggg cgtgtacggt gtaggccagt tcgcagcccg
      181 tgccctgcgg cgtcagcgtc acgccggaat ttcccttgcc gaatccggcc gcgccgccct
      241 ggccgtcaaa gctgatggtg taactgtcgg gcggcttgat atccgagagg ccgatcttgc
      301 cggcgaacct ggcggccagc ggtccgatct tgaccgccac gccgatagcg tattggttct
      361 cgcccgtggg ctcgaacttg tcgcagccgg gaatgcaggc cctgagcact tgcggattgt
      421 tgagcgcatc ccaggcctgc tgttgcgtga ccgccagcgc gcggctgcct tgcatgtcca
      481 tgattttcag attcctcagt gaagaggcag ccggtctgcg cggctttttg cgcaggtccg
      541 gctgaccgtc aggaggtcaa tgcgaatcgc tccgggcgcg gccggccgat tctccgacca
      601 ccactccgca gctctttcta tctggcgctg agcgcgcggc ttgattcgga caacgagcgc
      661 caagatcagc cgaatcgctg gaggcgggcg aagagttcct cggcagatat gccttcttcg
      721 cggtcggctt catcaagtgc ttcgagaagc tgagcctctt cggccggtga aagacgcacg
      781 gcctgctcgt cgtcacgggt cagaaccgta acgacagttc cctcaggcag ggcgagtcct
      841 tcgatcacga ccttgccatc gataacggtg ccagtagcta gctgcataaa acaatggtac
      901 tccaaggcgg cgcagaacgc gagactgcca tcgaacgtga aagtaaccgg cgaccaggga
      961 aaggtgcgcg cgcagcgcgc ggatgagaaa ggcgacgtca cgcaatttgg tggtgctcga
     1021 accgagtgcc tagcgcttca tcagggccgc gaggctggcg gccaactcct cgagcttgct
     1081 gatgttgtgc accgcgagca tgccgtgcgc ctgacgatgc agcacggcgg cgccgcgcgc
     1141 cgtcggcgca tagccgtcga aacgcagcag gggattgagc cagagcagac gccggctgcg
     1201 cagtcgcagc cagtcgagtt cgcgctccag ttcgtccggc gctccggtgt ccaggccgtc
     1261 cgtgatcgcc agcaccagag tgcggcggcc gaccagtcgg cgcgcgtgct gctggcgcag
     1321 cgtggccagc gattcaccca ggcgcgtgcc tccggcgaaa tcgctgatgg ccatgccggc
     1381 ggcagccagc atggcgtcgg tgtcggccag ccggaaggcc ggcgtcaggt ccgtgaggtg
     1441 ggtcccgaaa gcgaacacgt cgcgccgccc gacccggccg ccgccgcccc gggtagccgc
     1501 atgcagaaac gccagcagca ggcgcgcgta gcgctccatc gagcccgaga catccaccag
     1561 caccagcagc ggcaggggct gctggtggcg ctgcagccgc tgcaactgaa ccagttcgcc
     1621 accggtgccc gccgcacggc gcatcacacc gggccagtgc acccgcaagc cgcgctcccc
     1681 agggtgcagg cgccgcgagg gcacgcaggg aatgggcaag gcgatgtcgc gcgcgagtcg
     1741 ctcgatcagg ccgtactcgc cggcgctcaa cgcattgaag tcggcatgct tgagtcgcac
     1801 caggtcgctg gccgtcatcg cggcgtcgaa gtcgaccttc tggtcctgtt ttgtcggccg
     1861 gcttgggccg aacgcctgtt gcggcgaaag cgcttcgcgc accctcgggc ggcgcttgct
     1921 cggctcagcc ctgccttcgg cgctgggcag caactgcgcc agcagcttgt tggcgacctc
     1981 ggggtcgcgg aaataagcgt cgaacagctc gcgaaacacc agccggtcct gctcccggct
     2041 gaccatcacg gcctccatcg cggcgccgag gtcgtccttg ctggacaccc ccaccaactg
     2101 cgccgcctgc tgcgccagcg cgatgcgcgc gctgtccgtg cgcacgccgg cccggcgcag
     2161 ggcacggccg aaccccgtga tgttgtcggc cagctttccg gtgcgcgcgt caccgagctg
     2221 catgggacgc cctcacccta accctctccc agagggagag ggaacaagag aactccctat
     2281 ccccgctggg ataggaggac tccctctccc tgtgggagtg agcacaagag aactccttct
     2341 ccctgtggga gagggcaggg gcgagggcat ggggttcatg ccacctcctc tggcttgagc
     2401 aactcagtcg ccaggtcgcg cgtgagcgcg gccacgtctt cgcgctgctt gaacaggatg
     2461 ccggccgtat ccgccacgac ttcgggatcg accaccagcg tgtccagtgc caccagggcc
     2521 ttggcccact cgacgctttc ggcaatgccg ggtgcgcgct ggaacgcgtc ggcaaatggc
     2581 tggctgcgca acttgccgac gaagccggcc acctgccgtg ccagcgcctc gctggccccc
     2641 ggtacctgcg cccgcacgat ctccagttcg cggtcccggt ccgggtagtc cagccagtgg
     2701 tacaggcagc ggcgcttgac ggcgtcgttg agttcgcgcg tgcggttgct ggtcaggatc
     2761 gtgaccggcg tggccacggc gcgcaccgtc ccgatctcgg gaatgctcac ctggtattcg
     2821 cccaggtatt ccagcaggaa agcctcgaac ggctcgtcgg cgcggtcgac ctcgtcgatc
     2881 agcagcacgg cgccgggcgc cggcgcctgc agtgcctgca gcaggggacg gcggatgagg
     2941 tagcggtcct gatagacctc gcgttccacc tcggccgtgt ccgctggccc cccgtgccgc
     3001 atctcggcag cgcgcatgtg cagcaactgc gcggcgtagt tccactcgta cagggcttcg
     3061 cgctgctcca ggccgtcgta acactgcagg cgcagcaagt cccgtggcag ggcaagggcc
     3121 agcgccttgg ccagttcggt cttgcccacg cccggctcgc cttcgagcag caagggccgc
     3181 tgcagccgca gcgccaggaa taccgccgtc gccagccgcc ggtcggcaaa gtagccaacg
     3241 ccctgcagcc cctgcgtgag tgcgtcgatg gaggcgaaca ctcagacttt cgccacggcg
     3301 cgctgcgtca tcacgccgat caggttggcc cgataggctg ccgatgcgtg gatatcgctg
     3361 ctgagatcgc cggcgtcgat cttcacagcg gctgccgctg ccggcgtgaa gctcttgttc
     3421 aacgcgtctt ccagcccctt gtgacggaac accccgttgc cgccgccggt cacagcgacg
     3481 cgcacgccgc cgtcggtctg cgcgacgaac acgccgatca gcgcaaagcg cgaggccggt
     3541 tgcttgaact tcatgtaggc ggcgcgcttg ggcaccggaa agctgatcgc cgtgatcagt
     3601 tcatcttccg cgagcgccgt ggtgaacagc cccaagaaga agtcgtcggc ggcgatctgg
     3661 cgtttcgtgg tctgcacggt agcgcccagg ccgagcaccg cgctggggaa gcaggccgcg
     3721 ggatcgttgt tggccaccga gccgcccagc gtgcccagcg cgcgcacctg cttgtcgccg
     3781 atgtggccag ccaggtcggc cagcgcgggg atggcggtct tgaccacgtc gctctcagcg
     3841 acttcggcat ggcgcgccat cgcgccgatc acgagattgt tgccgtcctt gcggataccc
     3901 gccagctcct tgatgccgcc gaggtcggcc aaagtgccag gactggacaa gcgaagcttc
     3961 atcgacgcca gcagggtctg gccgccggcc agcggtgtcg cgccggacgc agccaggcgg
     4021 gccgcatcgg ccgcggtcgt gggacgttcg aaggtgaatg catacatggt catctctccc
     4081 tatggcctgg ccgcctggat cgcttcccag acgcggctgg gcgaagcggg catatcgaga
     4141 tccttcacgc ccaggtcgcg cagggcgtca agcacggcgt tgatcaccgc cggcggcgag
     4201 ccgatcgcgc cggcttcgcc gcagcctttg gtgcccaacg ggttgtggct gcaaggcgtg
     4261 cacacagtgt ccactttgaa catcgggaaa tcgtcggcgc gcggcatggc gtagtccatg
     4321 aaactgccgg tgagcaactg gccggactcg gggtcgtaca cgcagttttc cagcagcgcc
     4381 tggccgatcc cctgcacaag tccgccgtgc acctggcctt cgacgatcat cggattgatg
     4441 atggtgccga agtcatccac cgccgtgaaa cggtcgatcc gggtagtgcc ggtggcagtg
     4501 tccacttcca cttcgcagat gtaggtgccg gccgggaagg tgaagttggt cgggtcgtag
     4561 aacgcggtct cgttcaagcc cggctcgagc ttgtccagcg ggtagttgtg cggcacgtag
     4621 gccgtcagcg cgacctggcc aaaggtgacc ttcttgtccg tgcctttgac ggtgaattcg
     4681 ccgttggcga attcgacgtc ggcatcgctg gcttccatca ggtgcgcggc gatcttcttg
     4741 gccttggtct cgatcttgtc cagcgcccgc atgatcgccg caccgcccac gctgatcgag
     4801 cgcgacccgt aggtgcccat gccgaacggc acacggccgg tgtcgccgtg caccacgtcc
     4861 acgttctcca ccgtgatgcc caggcgcgcg gccaccacct gggcaaaggt ggtttcatgg
     4921 ccctggccgt ggctgtgcga gccggtgaac accgtgacgc tgccggtggg gtggacacga
     4981 atttcgccgc actcgaacaa gcccgcgcgc gcgcccagcg cgcccgcgat gttgctcggc
     5041 gcgatgccgc aggcctcgat gtagctgctg tagccgatgc cacgcttcaa accgttggcg
     5101 gcgctggccg ccttgcgggc atcgaagccg gccacctcgg ccagcgcctt ggccttgtcc
     5161 atgcaagcgt ggtaatcgcc cacgtcgtac tgcagcgcca ccggcgtctg gtagggaaag
     5221 ctggtgatga agttgcgctt gcggatttcg tcctggccca ggcccagttc ccaggcgcag
     5281 cggctgacca ggcgctccag caggtaggtg gcctcggggc ggcccgcgcc acggtaggcg
     5341 tcgaccggcg cggtgttggt gaaccaggca tcgacttcca catggatctg cggcgtggtg
     5401 tactcgccgg ccagcaaggt cgcatagagg atggtgggca ccgcggtcga gaaggtggaa
     5461 aggtacgcgc cgaggttggc gtcggtatgc acccgaagcg ccaggaactt gccgtccttg
     5521 tccatggcca tttcggcatg gctgacgtgg tcgcggccgt gcgcgtcgca caggaagctc
     5581 tcgctgcgct cggccgtcca cttgattgcg cagttgagct gcttggccgc ccaggtcagc
     5641 gccacgtcct cggcgtacag gaagatcttg gagccgaagc cgccgcccac gtcaggagcg
     5701 atcacgcgca ccttgtgctc gggcaggctc agcacgaatg ccgtcatcag cagccgctcg
     5761 acgtgcgggt tctgattggc cacgtacagc gtgtattcgt cggtcgcgcg gttgtacgaa
     5821 ccgatcgcgg cacgcggttc gatggcgttg ggtatcaaac ggttgttgac caggtccagc
     5881 cgggtgacgt gtgcggccga ggcaaacacg gcatcgaccg cggccttgtc gccgatggcc
     5941 catttgtagc agtggttgtc cggcgcggct tcatgcaggg ccggaccgtt cttcgcgtcc
     6001 tgcacacgga ccacggccgc cagcacctcg tagtcgactt ccaccgcctc cgcggcattc
     6061 ttggcgtgtt cgatggtgtc ggctaccacc atcgccacat ggtcaccgac atagcgcacc
     6121 ttgccctgcg ccaggatcgg gtgcggcggt tctttcatcg gctggccgtc ggtgctggtg
     6181 atcagccagc cgcaaggcag gcctcccatc ttgccttcca cgtccttgcc ggtgaagacc
     6241 ttgaccacgc cgggcagggc ctgcgccttg gcgacgtcga tgcccttgat cacggcatgc
     6301 gcgtgcgggg aacgcacgaa caccgcgtaa ttctggtggc ccaggttcac gtcgtccgtg
     6361 tactggccgg cgccggtcag gaaccggtag tcttccttgc gcctgagcgc ctcgccgatg
     6421 tgcggcagct tggagaaatc ggatgcaccc atgttcgttt ctccttcagg ccgaagccat
     6481 ggccgtctgg cccagttgca cggccttgac gatgttctgg tagccggtgc agcggcacag
     6541 gttaccttcg agcagttcgc ggatttcggc ttccccggcg cccgggttgt gctggcacag
     6601 gtccacggcg ctcatcacca tgcccggcgt gcaaaagccg cactgcaggc cgtggcattc
     6661 cttgaaggcc gcctgcatcg ggtgcatcgt cccgtccgcc gcggccaggc cctcgatcgt
     6721 ggtgacctcg gcgccttcat gctgcacggc cagcgtgttg cacgatttga tggcccggcc
     6781 attcacgatg acggtgcagg cgccgcattg ggcggtgtcg cagccgacgt gggtgccggt
     6841 gagctggagg tgttcgcgca gcacatgcac cagcagtgtg ttgggtgcga tgtcaacgct
     6901 ggcggacttg ccattgaccg tgagttgaac ttgcattgcg cttgtctcct tcaagggaat
     6961 gtcgcgaatt ggcggcgtgc cggagccgga ttcattgttt gaccctccca ggtgcgctaa
     7021 cctaggcgaa accctcgaac atccacgcgg cgacacgagg tgacaccaaa ttctcaaaat
     7081 ggaacaatag agccccgtgc cggaacgccc aacctgcgac ccattgtcga gccgtccatg
     7141 acagtcccca tccaaacgat tgccgagcag cgcctgcggc agatcgaact ggcgcgccgc
     7201 gccgtcatgg tcgaaggccg cagcgccacc gacgccctgg cccatgcctg gttcgaaggc
     7261 cactggattg aacgctcgtg gcggcgttgc ctggccagcg gccagcggcc ggagcaacgg
     7321 cttggcttcg acatgatcgc ggcgcaggcg cggcagcgcg tgatcgacgc cagccagccg
     7381 ttggtgcgcg ccgcccgccc ggtgctcgac cgcctgtccc gcgcgctggc cagcacccgc
     7441 tactttgcga tcctcaccga ccacgacggc atcgtcatcg atgcccacgg gccgatcgac
     7501 cgcagcgacc gccgagccga cctgatcacc cgcatcggtg tcgatttgtc cgagcgcagc
     7561 gtcggcacca gcgccatcgg cgccgcgctg gccgagctgc agcccgtctg gctgcaccgc
     7621 ggcgagcatt tcttcgccga cacttcggtc tacagttgtg ccggcgcgcc gcttttcggg
     7681 cccgacgggc gttgcgtcgg catgctcgac ctgaccggca tcaacgcggc tgaacggccg
     7741 gaactcaagc acctggtcgc gcaatcggca cgcagcatcg agaacgcact cacgctggcc
     7801 g
//
LOCUS       DASWIY010000504         4008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Ramlibacter sp. isolate SMAG_U2881 k141_3691130, whole
            genome shotgun sequence.
ACCESSION   DASWIY010000504 DASWIY010000000
VERSION     DASWIY010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960527
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ramlibacter sp. (soil metagenome)
  ORGANISM  Ramlibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Ramlibacter.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:11:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,620
            CDSs (total)                      :: 3,583
            Genes (coding)                    :: 3,549
            CDSs (with protein)               :: 3,549
            Genes (RNA)                       :: 37
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 31 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 4 of 34
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Ramlibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3691130"
                     /isolate="SMAG_U2881"
                     /isolation_source="soil"
                     /db_xref="taxon:1917967"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..2589
                     /locus_tag="VGA59_18085"
     CDS             295..2589
                     /locus_tag="VGA59_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX9721632.1"
                     /translation="MAAELSEAEQALRDAAREYHQSPTRGKISVTPTKPLSNQRDLSL
                     AYSPGVAYPCLDIQAEPSKAHEYTSRGTLVGVITNGTAVLGLGDIGPLAGKPVMEGKG
                     CLFKKFAGIDVFDIELDEKDPDKIVDIVAALEPTLGGVNLEDIKAPECFYIEKKLRER
                     MNIPVFHDDQHGTAIITSAAMLNALELVGKNIAEVKIAVSGAGAAAIACLDVLVGLGV
                     KRRNVWACDSKGLIYEGRPGGYDESKARYAQNTDARTLADAVKDADVFLGCSAPGVLT
                     PEMCKTMAARPIILALANPEPEIRPELAKAVRPDCIIATGRSDYPNQVNNVLCFPYIF
                     RGALDCGATKITEAMKLAAVREIAELAKADISEEVANAYAGKELSFGPEYLIPTPFDS
                     RLILRIAPAVAKAAAESGVATRPITDFDAYRQSLTRFVYQTGMIMQPVFQAAKAAATK
                     RVVFAEGEDDRVLRAVQLALEDGLAQPILIGRPAVIEARIAKAGLRMKLGKDFDCVNP
                     ESDTRFRQYWEAYHHRMGRRGVTPEAAKAAVRRSNTLIGSLAVELGHADAMLCGLVGR
                     FDAHLEHVCNVVGLAKGAPGFATLNALMLQDRTLFIADTYVNEDPSAELLAGIAAMAV
                     EEVRRFGLPPKVAFLSHSNYGTSSRASARKMRLARDLFARMAPDVECDGEMQGDSALS
                     ETVRRGSLMETTLTGEANLLICPNLDSANILFNVLKMTGGHGITVGPILLGAAKAVHV
                     LTPSSTVRRVVNMTALAAAGVAYS"
     gene            complement(2627..2842)
                     /locus_tag="VGA59_18090"
     CDS             complement(2627..2842)
                     /locus_tag="VGA59_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX9721633.1"
                     /translation="MTRKYVDCREIPSESNCSVAIAADSDQELVEAAVQHAVAVHKHQ
                     DTPEFRQQIKQAFHEGTPPIERPAHAK"
     gene            complement(3151..3417)
                     /locus_tag="VGA59_18095"
     CDS             complement(3151..3417)
                     /locus_tag="VGA59_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX9721634.1"
                     /translation="MSFILWLIVGGVVGWLASLIMKTDGQQGILLNVVVGIVGAFIGG
                     WLISPMVGMGTINEGFSIGSLVVSLIGAVILLAIVNLFRRGRVR"
     gene            3856..>4008
                     /locus_tag="VGA59_18100"
     CDS             3856..>4008
                     /locus_tag="VGA59_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX9721635.1"
                     /translation="MAWNARLALDYRLEGGRCVARFEHQGPLRILQTLYPEGDGVCHN
                     VLVHPPG"
BASE COUNT          725 a         1309 c         1303 g          671 t
ORIGIN      
        1 gggcagaggt gcttgaagat ggcggggccg ccgcgaccag gcggaggaac acggcaaacg
       61 tcgatggcga gcatactttc aagccggccc ttcgggttgc gcttggtcaa aaagatcaga
      121 atctgcatgg gtttggcgca caaggcggga cgggagtgca cacagcgaaa tatgacgcag
      181 cacaaaggac tagcggaaag ccggtttttt tgaagccgat ttggcttcgg atgtagtcgc
      241 agcgcgcaat tgggtttagc gtatcggctc tgtcaagttc caaggagaca ccccatggct
      301 gccgaactgt ccgaagccga acaggcattg cgcgatgccg cgcgcgagta tcaccaatcg
      361 cccacgcggg gcaagatttc ggtcacgccg accaagccgt tgtcgaacca gcgcgatctg
      421 tcgctggcct attcccctgg cgttgcctac ccttgcctgg acatccaggc cgaaccgtcc
      481 aaggcgcacg aatacacctc gcgcggcact ctggtgggcg tgatcaccaa tggcacggcc
      541 gtgctcggcc tgggcgacat cggcccgctg gcgggcaagc ccgtgatgga aggcaagggg
      601 tgcctgttca agaagttcgc cggcatcgac gtgttcgaca tcgagctgga cgagaaagat
      661 ccggacaaga tcgtcgatat cgtggccgcg ctggagccca cgctcggcgg ggtcaacctc
      721 gaggacatca aggcgcccga gtgcttctac atcgagaaga agctgcgcga gcgcatgaac
      781 atcccggtgt tccacgacga ccagcacggc accgccatca ttacctccgc ggcgatgctc
      841 aacgcgctcg agctggtggg caagaatatt gccgaagtga aaatcgcggt ctcgggtgcc
      901 ggcgccgccg ccatcgcctg cctggatgtg ctggtgggcc tgggggtcaa gcgcaggaac
      961 gtctgggcgt gcgattccaa gggcctgatc tatgagggcc ggcccggcgg ctacgacgaa
     1021 tcgaaggcac gctacgcgca aaacacggac gcgcgaacgc tcgccgacgc ggtgaaggac
     1081 gccgatgttt tcctgggctg ctcggcgccg ggcgtgttga cacccgagat gtgcaagacg
     1141 atggcggcgc ggcctatcat cctcgcgctg gccaatcccg agcccgaaat ccggcccgaa
     1201 ctggccaaag ccgtccggcc cgactgcatc atcgcgaccg gccgatcgga ctaccccaac
     1261 caggtcaaca acgtcctgtg tttcccctac atctttcgcg gcgcactcga ctgcggcgcg
     1321 accaagatca cggaggcgat gaaattggct gccgtgcgcg agatcgccga gctggccaag
     1381 gccgacatca gcgaggaagt cgccaacgcc tacgccggca aggaactctc cttcggaccc
     1441 gagtacctga tccccacgcc tttcgactcg cggctgatcc tgcgcatcgc gccggcggtg
     1501 gcgaaggcgg ccgccgagtc cggcgtggcc acgcggccga tcaccgactt cgacgcctat
     1561 cgccagagcc tgacccgttt cgtttaccag accggcatga tcatgcagcc cgtgttccag
     1621 gccgccaagg cggccgcgac caagcgggtc gtgtttgccg agggtgaaga cgaccgcgtg
     1681 ctgcgcgccg tgcaactggc cctcgaagac ggcctggccc agccaatcct gatcggccgg
     1741 ccggccgtga tcgaggcgcg tatcgccaaa gcgggcctgc gcatgaagct gggcaaggac
     1801 ttcgactgcg tgaatccgga aagcgacacg cgcttccgcc agtactggga ggcctaccac
     1861 caccggatgg gccggcgcgg cgtgacgccc gaggccgcga aggccgcggt ccgccgctcc
     1921 aacaccttga tcggctccct ggccgtggag ctgggccacg cggacgccat gctgtgcggg
     1981 ctggtcgggc gcttcgacgc gcacctggaa cacgtgtgca acgtggtcgg gctcgccaag
     2041 ggcgcacccg gcttcgcgac gctgaatgcg ctgatgctgc aggaccgcac actgtttatc
     2101 gccgacacct acgtcaacga ggatcccagt gctgaattgc tggccggcat cgccgccatg
     2161 gccgtggaag aagtgaggcg ttttgggctg ccgcccaagg tcgccttcct ctcgcacagc
     2221 aactacggca cgtccagccg tgcctcggcg cgcaagatgc ggctggcgcg cgacctgttc
     2281 gctcgcatgg cgcccgacgt ggagtgcgac ggcgagatgc agggcgattc ggccctttcc
     2341 gagaccgtgc gtcgcggcag cctgatggaa accacgctga ccggcgaggc caacctgctg
     2401 atctgtccca acctcgattc ggccaacatt cttttcaatg tgctgaagat gacgggaggc
     2461 cacggcatca cggtgggccc gatcctgctt ggcgccgcca aggccgtgca tgtgctcacg
     2521 ccctcctcca ctgtgcggcg cgtggtcaac atgaccgcgc tggccgcggc gggggtggct
     2581 tactcctgaa gacgaggcgg ctcaatgccg cctcgcgcgg cgctcgtcac ttcgcgtgag
     2641 ccgggcgctc gatcggcggt gtgccttcgt ggaaagcctg cttgatctgc tggcggaatt
     2701 ccggcgtgtc ctggtgcttg tgaaccgcca cagcgtgctg caccgccgcc tcgaccagtt
     2761 cctggtcgct gtccgcggca atagcgaccg agcaattgct ttcgctggga atttcgcggc
     2821 aatcgacata tttgcgtgtc atagggcctc ctgatgcgca gggtggacgg agtgtgcccc
     2881 cgacggatga gccggtcacc tcttcaggag cgtaatgcgg cgcggccgca atctgatcgt
     2941 tatagtccgc gcacactgct ttttcaacgg ggcccgggcc cctggctgct gggccgtcgt
     3001 gtatgcgcct tcctcaagaa cgccagacgc gggctgaaga gcgccaacag ccagcccaga
     3061 cgcgacaacg cgtgcgccaa gtagccgcac gcgcgaaaga aggcatccgg ccgaaaggcc
     3121 ggatacccga aaaccttgcc ttgcagggcg ttagcggacc cgaccgcggc ggaacagatt
     3181 gacgatcgcc agcaggatca cggcaccgat cagcgacacg accagcgagc cgatgctgaa
     3241 tccttcattg atcgtgccca tgccgaccat cggggaaatc agccagccgc cgatgaaggc
     3301 gccgacgata cccacgacca cgttcagcag gatgccctgc tgaccatcgg tcttcatgat
     3361 caggctggcg agccagccga cgacaccgcc tacgattagc cagagtatga aactcatatg
     3421 agactccttt gccgcgaacg gcgtgttgtt gatagcgggc cggtccctca cggttccgac
     3481 caccgccgcc aagaacgaac ggccggtgca ggcaatatgg tccgcacccc ccggcggtgg
     3541 cgtagtcgcc agtaaaggct cagcgtgcgc gcttgtcgca tggcggcgtc ggacgcgccc
     3601 tactcctcag ccgtggtttc gcgaagcggc aagcaagcga tcatggccac ttcgccatgc
     3661 ggcgaaaatg aggctttccg gtccggccgc acaccccgtt ggttcatatg cgtttttccg
     3721 tctttcctgt gcctggagcg tccttcttca gcatctgtgc cggcctcggc aggctcgagc
     3781 tcgggctgga cagcgtgcca cgctgcgtga tcggccggag ggacggggcg gcgcgcggcg
     3841 cgccgggcgc ttgagatggc ctggaacgcg cggctggcgc tcgactaccg gctcgaaggc
     3901 ggccgctgcg tggcgcgctt cgaacatcag gggcctctgc gcattctgca aacgctttat
     3961 cccgagggcg acggcgtctg ccacaacgtg ctggtgcacc cgcccggc
//
